LOCUS       DAZFXT010000001       109826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.001, whole genome shotgun sequence.
ACCESSION   DAZFXT010000001 DAZFXT010000000
VERSION     DAZFXT010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 109826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 109826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..109826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.001"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            588..1037
                     /gene="alaE"
                     /locus_tag="ACHKW0_000001"
     CDS             588..1037
                     /gene="alaE"
                     /locus_tag="ACHKW0_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HFO7472904.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(1074..1418)
                     /gene="ygaC"
                     /locus_tag="ACHKW0_000002"
     CDS             complement(1074..1418)
                     /gene="ygaC"
                     /locus_tag="ACHKW0_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2002 family protein"
                     /protein_id="HFO7472905.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1570..1899
                     /gene="ygaM"
                     /locus_tag="ACHKW0_000003"
     CDS             1570..1899
                     /gene="ygaM"
                     /locus_tag="ACHKW0_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HFO7472906.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            2147..2392
                     /gene="nrdH"
                     /locus_tag="ACHKW0_000004"
     CDS             2147..2392
                     /gene="nrdH"
                     /locus_tag="ACHKW0_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HFO7472907.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2389..2799
                     /gene="nrdI"
                     /locus_tag="ACHKW0_000005"
     CDS             2389..2799
                     /gene="nrdI"
                     /locus_tag="ACHKW0_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HFO7472908.1"
                     /translation="MSQLVYFSSNSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2772..4916
                     /gene="nrdE"
                     /locus_tag="ACHKW0_000006"
     CDS             2772..4916
                     /gene="nrdE"
                     /locus_tag="ACHKW0_000006"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7472909.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADVAISQHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4926..5885
                     /gene="nrdF"
                     /locus_tag="ACHKW0_000007"
     CDS             4926..5885
                     /gene="nrdF"
                     /locus_tag="ACHKW0_000007"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005971 - ribonucleoside-diphosphate
                     reductase complex [Evidence IEA];
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7472910.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            6240..7442
                     /gene="proV"
                     /locus_tag="ACHKW0_000008"
     CDS             6240..7442
                     /gene="proV"
                     /locus_tag="ACHKW0_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0031460 - glycine betaine transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HFO7472911.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7435..8499
                     /gene="proW"
                     /locus_tag="ACHKW0_000009"
     CDS             7435..8499
                     /gene="proW"
                     /locus_tag="ACHKW0_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HFO7472912.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8557..9549
                     /gene="proX"
                     /locus_tag="ACHKW0_000010"
     CDS             8557..9549
                     /gene="proX"
                     /locus_tag="ACHKW0_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HFO7472913.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTHTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9841..11025
                     /locus_tag="ACHKW0_000011"
     CDS             9841..11025
                     /locus_tag="ACHKW0_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7472914.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            11149..11886
                     /gene="ygaZ"
                     /locus_tag="ACHKW0_000012"
     CDS             11149..11886
                     /gene="ygaZ"
                     /locus_tag="ACHKW0_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HFO7472915.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWIAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11876..12211
                     /gene="ygaH"
                     /locus_tag="ACHKW0_000013"
     CDS             11876..12211
                     /gene="ygaH"
                     /locus_tag="ACHKW0_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HFO7472916.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLG
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            12302..12832
                     /gene="emrR"
                     /locus_tag="ACHKW0_000014"
     CDS             12302..12832
                     /gene="emrR"
                     /locus_tag="ACHKW0_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HFO7472917.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHLLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12959..14131
                     /gene="emrA"
                     /locus_tag="ACHKW0_000015"
     CDS             12959..14131
                     /gene="emrA"
                     /locus_tag="ACHKW0_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HFO7472918.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            14148..15686
                     /gene="emrB"
                     /locus_tag="ACHKW0_000016"
     CDS             14148..15686
                     /gene="emrB"
                     /locus_tag="ACHKW0_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HFO7472919.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15750..16265)
                     /gene="luxS"
                     /locus_tag="ACHKW0_000017"
     CDS             complement(15750..16265)
                     /gene="luxS"
                     /locus_tag="ACHKW0_000017"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HFO7472920.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPEEKLQELHI"
     gene            complement(16415..17971)
                     /gene="gshA"
                     /locus_tag="ACHKW0_000018"
     CDS             complement(16415..17971)
                     /gene="gshA"
                     /locus_tag="ACHKW0_000018"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HFO7472921.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(18044..18472)
                     /gene="yqaA"
                     /locus_tag="ACHKW0_000019"
     CDS             complement(18044..18472)
                     /gene="yqaA"
                     /locus_tag="ACHKW0_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HFO7472922.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18469..19035)
                     /gene="yqaB"
                     /locus_tag="ACHKW0_000020"
     CDS             complement(18469..19035)
                     /gene="yqaB"
                     /locus_tag="ACHKW0_000020"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HFO7472923.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(19316..19392)
                     /locus_tag="ACHKW0_000021"
     tRNA            complement(19316..19392)
                     /locus_tag="ACHKW0_000021"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19356..19358),aa:Arg,seq:acg)
     gene            complement(19591..19667)
                     /locus_tag="ACHKW0_000022"
     tRNA            complement(19591..19667)
                     /locus_tag="ACHKW0_000022"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19631..19633),aa:Arg,seq:acg)
     gene            complement(19731..19807)
                     /locus_tag="ACHKW0_000023"
     tRNA            complement(19731..19807)
                     /locus_tag="ACHKW0_000023"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19771..19773),aa:Arg,seq:acg)
     gene            complement(20005..20081)
                     /locus_tag="ACHKW0_000024"
     tRNA            complement(20005..20081)
                     /locus_tag="ACHKW0_000024"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20045..20047),aa:Arg,seq:acg)
     gene            complement(20085..20177)
                     /locus_tag="ACHKW0_000025"
     tRNA            complement(20085..20177)
                     /locus_tag="ACHKW0_000025"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20141..20143),aa:Ser,seq:gct)
     gene            complement(20493..20678)
                     /gene="csrA"
                     /locus_tag="ACHKW0_000026"
     CDS             complement(20493..20678)
                     /gene="csrA"
                     /locus_tag="ACHKW0_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HFO7472924.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(20913..23543)
                     /gene="alaS"
                     /locus_tag="ACHKW0_000027"
     CDS             complement(20913..23543)
                     /gene="alaS"
                     /locus_tag="ACHKW0_000027"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HFO7472925.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(23671..24171)
                     /gene="recX"
                     /locus_tag="ACHKW0_000028"
     CDS             complement(23671..24171)
                     /gene="recX"
                     /locus_tag="ACHKW0_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HFO7472926.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(24414..25475)
                     /gene="recA"
                     /locus_tag="ACHKW0_000029"
     CDS             complement(24414..25475)
                     /gene="recA"
                     /locus_tag="ACHKW0_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HFO7472927.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(25555..26052)
                     /gene="pncC"
                     /locus_tag="ACHKW0_000030"
     CDS             complement(25555..26052)
                     /gene="pncC"
                     /locus_tag="ACHKW0_000030"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HFO7472928.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(26197..27282)
                     /gene="mltB"
                     /locus_tag="ACHKW0_000031"
     CDS             complement(26197..27282)
                     /gene="mltB"
                     /locus_tag="ACHKW0_000031"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HFO7472929.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNRSTHYAMAVWQLGQAVALARVQ"
     gene            27538..28101
                     /gene="srlA"
                     /locus_tag="ACHKW0_000032"
                     /gene_synonym="gutA"
     CDS             27538..28101
                     /gene="srlA"
                     /locus_tag="ACHKW0_000032"
                     /gene_synonym="gutA"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HFO7472930.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            28098..29057
                     /gene="srlE"
                     /locus_tag="ACHKW0_000033"
                     /gene_synonym="gutE"
     CDS             28098..29057
                     /gene="srlE"
                     /locus_tag="ACHKW0_000033"
                     /gene_synonym="gutE"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HFO7472931.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            29068..29439
                     /gene="srlB"
                     /locus_tag="ACHKW0_000034"
                     /gene_synonym="gutB"
     CDS             29068..29439
                     /gene="srlB"
                     /locus_tag="ACHKW0_000034"
                     /gene_synonym="gutB"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HFO7472932.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGELHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            29443..30222
                     /gene="srlD"
                     /locus_tag="ACHKW0_000035"
     CDS             29443..30222
                     /gene="srlD"
                     /locus_tag="ACHKW0_000035"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HFO7472933.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            30327..30686
                     /gene="gutM"
                     /locus_tag="ACHKW0_000036"
     CDS             30327..30686
                     /gene="gutM"
                     /locus_tag="ACHKW0_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HFO7472934.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            30753..31526
                     /gene="srlR"
                     /locus_tag="ACHKW0_000037"
                     /gene_synonym="gutR"
     CDS             30753..31526
                     /gene="srlR"
                     /locus_tag="ACHKW0_000037"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HFO7472935.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            31519..32484
                     /gene="gutQ"
                     /locus_tag="ACHKW0_000038"
     CDS             31519..32484
                     /gene="gutQ"
                     /locus_tag="ACHKW0_000038"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HFO7472936.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALAASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(32481..33995)
                     /gene="norR"
                     /locus_tag="ACHKW0_000039"
     CDS             complement(32481..33995)
                     /gene="norR"
                     /locus_tag="ACHKW0_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HFO7472937.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDAAPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLIYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLSRATRSGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            34182..35621
                     /gene="norV"
                     /locus_tag="ACHKW0_000040"
     CDS             34182..35621
                     /gene="norV"
                     /locus_tag="ACHKW0_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016966 - nitric oxide reductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HFO7472938.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            35618..36751
                     /gene="norW"
                     /locus_tag="ACHKW0_000041"
     CDS             35618..36751
                     /gene="norW"
                     /locus_tag="ACHKW0_000041"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016731 - oxidoreductase activity, acting
                     on iron-sulfur proteins as donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HFO7472939.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLRLFPHTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRASETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(36977..39229)
                     /gene="hypF"
                     /locus_tag="ACHKW0_000042"
     CDS             complement(36977..39229)
                     /gene="hypF"
                     /locus_tag="ACHKW0_000042"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HFO7472940.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDVHPGYVSSQWASEMNMPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAATQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(39382..39909)
                     /gene="hydN"
                     /locus_tag="ACHKW0_000043"
     CDS             complement(39382..39909)
                     /gene="hydN"
                     /locus_tag="ACHKW0_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HFO7472941.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(40058..41068)
                     /gene="ascG"
                     /locus_tag="ACHKW0_000044"
     CDS             complement(40058..41068)
                     /gene="ascG"
                     /locus_tag="ACHKW0_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HFO7472942.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            41328..42785
                     /gene="ascF"
                     /locus_tag="ACHKW0_000045"
     CDS             41328..42785
                     /gene="ascF"
                     /locus_tag="ACHKW0_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HFO7472943.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALISTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEATAEARKHQSVQPTVAKEVSLN"
     gene            42794..44218
                     /gene="ascB"
                     /locus_tag="ACHKW0_000046"
     CDS             42794..44218
                     /gene="ascB"
                     /locus_tag="ACHKW0_000046"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HFO7472944.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     KITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(44332..44751)
                     /locus_tag="ACHKW0_000047"
     CDS             complement(44332..44751)
                     /locus_tag="ACHKW0_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7472945.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNEKFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(44822..45576)
                     /locus_tag="ACHKW0_000048"
                     /pseudo
     CDS             complement(44822..45576)
                     /locus_tag="ACHKW0_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001374644.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(45619..46089)
                     /gene="hycI"
                     /locus_tag="ACHKW0_000049"
     CDS             complement(45619..46089)
                     /gene="hycI"
                     /locus_tag="ACHKW0_000049"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HFO7472946.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            complement(46082..46492)
                     /gene="hycH"
                     /locus_tag="ACHKW0_000050"
     CDS             complement(46082..46492)
                     /gene="hycH"
                     /locus_tag="ACHKW0_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HFO7472947.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(46489..47256)
                     /gene="hycG"
                     /locus_tag="ACHKW0_000051"
     CDS             complement(46489..47256)
                     /gene="hycG"
                     /locus_tag="ACHKW0_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HFO7472948.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(47256..47798)
                     /gene="hycF"
                     /locus_tag="ACHKW0_000052"
     CDS             complement(47256..47798)
                     /gene="hycF"
                     /locus_tag="ACHKW0_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HFO7472949.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(47808..49517)
                     /gene="hycE"
                     /locus_tag="ACHKW0_000053"
     CDS             complement(47808..49517)
                     /gene="hycE"
                     /locus_tag="ACHKW0_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HFO7472950.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(49535..50458)
                     /gene="hycD"
                     /locus_tag="ACHKW0_000054"
     CDS             complement(49535..50458)
                     /gene="hycD"
                     /locus_tag="ACHKW0_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HFO7472951.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(50461..52287)
                     /gene="hycC"
                     /locus_tag="ACHKW0_000055"
     CDS             complement(50461..52287)
                     /gene="hycC"
                     /locus_tag="ACHKW0_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HFO7472952.1"
                     /translation="MSAISLINSGVAWFIAAAVLAFLFSFQKTLSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGILTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(52284..52895)
                     /gene="hycB"
                     /locus_tag="ACHKW0_000056"
     CDS             complement(52284..52895)
                     /gene="hycB"
                     /locus_tag="ACHKW0_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HFO7472953.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(53020..53481)
                     /gene="hycA"
                     /locus_tag="ACHKW0_000057"
     CDS             complement(53020..53481)
                     /gene="hycA"
                     /locus_tag="ACHKW0_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HFO7472954.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            53693..54043
                     /gene="hypA"
                     /locus_tag="ACHKW0_000058"
     CDS             53693..54043
                     /gene="hypA"
                     /locus_tag="ACHKW0_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7472955.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            54047..54919
                     /gene="hypB"
                     /locus_tag="ACHKW0_000059"
     CDS             54047..54919
                     /gene="hypB"
                     /locus_tag="ACHKW0_000059"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HFO7472956.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            54910..55182
                     /gene="hypC"
                     /locus_tag="ACHKW0_000060"
     CDS             54910..55182
                     /gene="hypC"
                     /locus_tag="ACHKW0_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HFO7472957.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            55182..56303
                     /gene="hypD"
                     /locus_tag="ACHKW0_000061"
     CDS             55182..56303
                     /gene="hypD"
                     /locus_tag="ACHKW0_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HFO7472958.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            56300..57310
                     /gene="hypE"
                     /locus_tag="ACHKW0_000062"
     CDS             56300..57310
                     /gene="hypE"
                     /locus_tag="ACHKW0_000062"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HFO7472959.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLELA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            57384..59462
                     /gene="flhA"
                     /locus_tag="ACHKW0_000063"
     CDS             57384..59462
                     /gene="flhA"
                     /locus_tag="ACHKW0_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HFO7472960.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRRILSRPDTLHCSYEEFC
                     ETWPQLVAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(59499..59852)
                     /gene="ygbA"
                     /locus_tag="ACHKW0_000064"
     CDS             complement(59499..59852)
                     /gene="ygbA"
                     /locus_tag="ACHKW0_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HFO7472961.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(59884..60063)
                     /locus_tag="ACHKW0_000065"
     CDS             complement(59884..60063)
                     /locus_tag="ACHKW0_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001363568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7472962.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVG
                     EPFFFRVIVPFKIKM"
     gene            60139..62700
                     /gene="mutS"
                     /locus_tag="ACHKW0_000066"
     CDS             60139..62700
                     /gene="mutS"
                     /locus_tag="ACHKW0_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HFO7472963.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            62806..63462
                     /gene="pphB"
                     /locus_tag="ACHKW0_000067"
     CDS             62806..63462
                     /gene="pphB"
                     /locus_tag="ACHKW0_000067"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7472964.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQKEATDLLLKFHHLPHIIEITNDNIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(63513..64280)
                     /gene="ygbI"
                     /locus_tag="ACHKW0_000068"
     CDS             complement(63513..64280)
                     /gene="ygbI"
                     /locus_tag="ACHKW0_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor YgbI"
                     /protein_id="HFO7472965.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            64476..65384
                     /gene="ltnD"
                     /locus_tag="ACHKW0_000069"
     CDS             64476..65384
                     /gene="ltnD"
                     /locus_tag="ACHKW0_000069"
                     /EC_number="1.1.1.411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonate dehydrogenase"
                     /protein_id="HFO7472966.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            65381..66643
                     /gene="otnK"
                     /locus_tag="ACHKW0_000070"
     CDS             65381..66643
                     /gene="otnK"
                     /locus_tag="ACHKW0_000070"
                     /EC_number="2.7.1.217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HFO7472967.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWTQENGNQARKAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLATYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVARFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            66640..67278
                     /gene="ygbL"
                     /locus_tag="ACHKW0_000071"
     CDS             66640..67278
                     /gene="ygbL"
                     /locus_tag="ACHKW0_000071"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HFO7472968.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            67283..68059
                     /gene="ygbM"
                     /locus_tag="ACHKW0_000072"
     CDS             67283..68059
                     /gene="ygbM"
                     /locus_tag="ACHKW0_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO7472969.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFAPHGKRILVEALSPGVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            68148..69512
                     /gene="ygbN"
                     /locus_tag="ACHKW0_000073"
     CDS             68148..69512
                     /gene="ygbN"
                     /locus_tag="ACHKW0_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HFO7472970.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(69551..69805)
                     /locus_tag="ACHKW0_000074"
     CDS             complement(69551..69805)
                     /locus_tag="ACHKW0_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit D"
                     /protein_id="HFO7472971.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRDT
                     EPLRRTSREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(69816..71243)
                     /locus_tag="ACHKW0_000075"
     CDS             complement(69816..71243)
                     /locus_tag="ACHKW0_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit C"
                     /protein_id="HFO7472972.1"
                     /translation="MAFDDLRSFLQALDDHGQLLKISEEVNAEPDLAAAANATGRIGD
                     GAPALWFDNIRGFTDARVTMNTIGSWQNHAISLGLPPNTPVKKQIDEFIRRWDNFPIA
                     PERRANPAWAQNTVDGEEINLFDILPLFRLNDGDGGFYLDKACVVSRDPLDPDNFGKQ
                     NVGIYRMEVKGKRKLGLQPVPMHDIALHLHKAEERGEDLPIAITLGNDPIITLMGATP
                     LKYDQSEYEMAGALRESPYPIATAPLTGFDVPWGSEVILEGVIESRKREIEGPFGEFT
                     GHYSGGRNMTVVRIDKVSYRTRPIFESLYLGMPWTEIDYLMGPATCVPLYQQLKAEFP
                     EVQAVNAMYTHGLLAIISTKKRYGGFARAVGLRAMTTPHGLGYVKMVIMVDEDVDPFN
                     LPQVMWALSSKVNPAGDLVQLPNMSVLELDPGSSPAGITDKLIIDATTPVAPDNRGHY
                     SQPVVDLPETKAWAEKLTAMLAARK"
     gene            complement(71243..71836)
                     /locus_tag="ACHKW0_000076"
     CDS             complement(71243..71836)
                     /locus_tag="ACHKW0_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-oxidative hydroxyarylic acid decarboxylases
                     subunit B"
                     /protein_id="HFO7472973.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            71983..72390
                     /locus_tag="ACHKW0_000077"
     CDS             71983..72390
                     /locus_tag="ACHKW0_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO7472974.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(72511..73503)
                     /gene="rpoS"
                     /locus_tag="ACHKW0_000078"
     CDS             complement(72511..73503)
                     /gene="rpoS"
                     /locus_tag="ACHKW0_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HFO7472975.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(73566..74660)
                     /gene="nlpD"
                     /locus_tag="ACHKW0_000079"
     CDS             complement(73566..74660)
                     /gene="nlpD"
                     /locus_tag="ACHKW0_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HFO7472976.1"
                     /translation="MSLVSLWLAGCSDTSNPPAPVSSVNGNAPANTNSGMLITPPPKM
                     GTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENGRIVYNRQYGNIPKGS
                     YSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVGQTLQVGNASGTPITG
                     GNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSANKMLPNNKPAATTVT
                     APVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASEGGNKGIDIAGSKGQA
                     IIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLVREQQEVKAGQKIATM
                     GSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(74845..75471)
                     /gene="pcm"
                     /locus_tag="ACHKW0_000080"
     CDS             complement(74845..75471)
                     /gene="pcm"
                     /locus_tag="ACHKW0_000080"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HFO7472977.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(75465..76226)
                     /gene="surE"
                     /locus_tag="ACHKW0_000081"
     CDS             complement(75465..76226)
                     /gene="surE"
                     /locus_tag="ACHKW0_000081"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HFO7472978.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(76207..77256)
                     /gene="truD"
                     /locus_tag="ACHKW0_000082"
     CDS             complement(76207..77256)
                     /gene="truD"
                     /locus_tag="ACHKW0_000082"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HFO7472979.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(77253..77732)
                     /gene="ispF"
                     /locus_tag="ACHKW0_000083"
     CDS             complement(77253..77732)
                     /gene="ispF"
                     /locus_tag="ACHKW0_000083"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HFO7472980.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(77732..78442)
                     /gene="ispD"
                     /locus_tag="ACHKW0_000084"
     CDS             complement(77732..78442)
                     /gene="ispD"
                     /locus_tag="ACHKW0_000084"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HFO7472981.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(78461..78772)
                     /gene="ftsB"
                     /locus_tag="ACHKW0_000085"
     CDS             complement(78461..78772)
                     /gene="ftsB"
                     /locus_tag="ACHKW0_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HFO7472982.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(78966..79289)
                     /gene="ygbE"
                     /locus_tag="ACHKW0_000086"
     CDS             complement(78966..79289)
                     /gene="ygbE"
                     /locus_tag="ACHKW0_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HFO7472983.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(79339..79944)
                     /gene="cysC"
                     /locus_tag="ACHKW0_000087"
     CDS             complement(79339..79944)
                     /gene="cysC"
                     /locus_tag="ACHKW0_000087"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004020 - adenylylsulfate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HFO7472984.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGVLEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(79944..81371)
                     /gene="cysN"
                     /locus_tag="ACHKW0_000088"
     CDS             complement(79944..81371)
                     /gene="cysN"
                     /locus_tag="ACHKW0_000088"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HFO7472985.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIRKDYLTFAEQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(81373..82281)
                     /gene="cysD"
                     /locus_tag="ACHKW0_000089"
     CDS             complement(81373..82281)
                     /gene="cysD"
                     /locus_tag="ACHKW0_000089"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HFO7472986.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            82533..83570
                     /gene="iap"
                     /locus_tag="ACHKW0_000090"
     CDS             82533..83570
                     /gene="iap"
                     /locus_tag="ACHKW0_000090"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HFO7472987.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     repeat_region   83591..84352
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=83713..83741
                     /rpt_unit_seq="cggtttatccccgctggcgcggggaacac"
     gene            complement(84449..84742)
                     /gene="cas2e"
                     /locus_tag="ACHKW0_000091"
     CDS             complement(84449..84742)
                     /gene="cas2e"
                     /locus_tag="ACHKW0_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2e"
                     /protein_id="HFO7472988.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(84742..85662)
                     /gene="cas1e"
                     /locus_tag="ACHKW0_000092"
     CDS             complement(84742..85662)
                     /gene="cas1e"
                     /locus_tag="ACHKW0_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1e"
                     /protein_id="HFO7472989.1"
                     /translation="MTFVPLSPIPLKDRTSMIFLQYGQIDVLDGAFVLIDKTGIRTHI
                     PVGSVACIMLEPGTRVSHAAVHLAATVGTLLVWVGEAGVRVYSSGQPGGARADKLLYQ
                     AKLALTEDLRLKVVRKMYELRFREPQPARRSVEQLRGIEGSRVRQTYALLAKQYGVKW
                     NGRKYDPKDWEKGDVVNRCISAATSCLYGISEAAVLAAGYAPAIGFIHSGKPLSFVYD
                     IADIIKFDSVVPKAFEIAARQPAEPDKEVRLACRDIFRSTKLTGKLIPLIEEVLAAGE
                     IEPPQPAPDMLPPAIPEPETLGDSGHRGRG"
     gene            complement(85659..86309)
                     /gene="cas6e"
                     /locus_tag="ACHKW0_000093"
     CDS             complement(85659..86309)
                     /gene="cas6e"
                     /locus_tag="ACHKW0_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HFO7472990.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGAFRFFVLSQERPAESDTFTIECRSFAPELCTGQQLCFNLRANPTICKA
                     GKRHDLLMEAKRQVRGQAEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQQQ
                     LRRENSRQLIQFSSVDYTGMLTVTDPGLFVQRLSQGYGKSRAFGCGLMLIKPGAEA"
     gene            complement(86291..87037)
                     /gene="cas5e"
                     /locus_tag="ACHKW0_000094"
     CDS             complement(86291..87037)
                     /gene="cas5e"
                     /locus_tag="ACHKW0_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA];
                     GO_process: GO:0051607 - defense response to virus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HFO7472991.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            complement(87048..88121)
                     /gene="cas7e"
                     /locus_tag="ACHKW0_000095"
     CDS             complement(87048..88121)
                     /gene="cas7e"
                     /locus_tag="ACHKW0_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HFO7472992.1"
                     /translation="MTTFIQLHLLTAYPAANLNRDDSGSPKTVILGGATRLRVSSQSL
                     KRAWRTSELFEQALAGHIGVRSGRIAREAATILIEKGIEDKKAIEWAVEIADYLGKAK
                     KDKKQKNDKKPKDPLTSAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEKDLALLRK
                     DRMAVDIAMFGRMLAKKPGFNVEAACQVAHAFGVSETIVENDFFTAVDDLRQASEDAG
                     AGHVDETGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNS
                     FASRAYASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKT
                     ECASFDVMNKQGSMKDVLDFICA"
     gene            complement(88136..88669)
                     /gene="casB"
                     /locus_tag="ACHKW0_000096"
                     /gene_synonym="cse2"
     CDS             complement(88136..88669)
                     /gene="casB"
                     /locus_tag="ACHKW0_000096"
                     /gene_synonym="cse2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HFO7472993.1"
                     /translation="MSIVKDEHKVALRKWHEELQDKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIAAVSANVKAIDERQPFAAQLAAVMKKGRFTRLL
                     AVKTPDDLLRQLRRAVKLLNGSVNLDSLAEGVFRWCQESDDLLNHHRRQQRPTEFIRI
                     RWALEYYQAGDADNEQN"
     gene            complement(88666..90228)
                     /gene="casA"
                     /locus_tag="ACHKW0_000097"
                     /gene_synonym="cse1"
     CDS             complement(88666..90228)
                     /gene="casA"
                     /locus_tag="ACHKW0_000097"
                     /gene_synonym="cse1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
                     /protein_id="HFO7472994.1"
                     /translation="MNSFSLLTTPWLPVRFKDGTTGKLAPVDLADENVVDIAAPRADL
                     QGAAWQFLLGLLQTSFAPKDQRRWDDIWEDGLEAEKLREALLSLEHAFQFGPDSPSFM
                     QDFDELKVKATSIASLLPDAPGKQTKERNTDHFIKRDTTQHLCLHCVPLALFSIQLNA
                     PIGGRGYYPGLRGGGPLTTLIELLEYQGNQQTPLWRKLWLNVMPQDEADLPLPKTFDD
                     LVFPWLAPTRTSELDGAVVTDEQVNKLQAYWGMPRRIRIDFKTTSIGNCDICGRQSDA
                     LLGLMSLKNYGVQYVMWRHPLTPYRLPLKEGGDFYSVKPQPGGLIWRDWLGLIEVGNS
                     KNNTELPAQVVKLLNASNLKQTRVGLWGFGFDFEDMKVRCWYEHHFPLLLNKKEDLIP
                     KLRLAAQAASHILSLLHRALKEAWFSEKKTTKLDFGFVDIDFWNKTQHRFLRLVRKIE
                     EGQDPYELLSKWQKEMWLFARQDFDDRVFTNPYEPVDLERVMTARKKYFTTSAEKQNA
                     NAAREKKQEAAE"
     gene            complement(90326..93025)
                     /locus_tag="ACHKW0_000098"
     CDS             complement(90326..93025)
                     /locus_tag="ACHKW0_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HFO7472995.1"
                     /translation="MRKYPLSLLKDKNIVTFFDFWGKTRRGEKDGGDDYHLLCWHSLD
                     VAAMGYLMVKRNCFGLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPP
                     ELKIQESARKNYEKISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTT
                     GHHGRPPDRMDELDNFLPEDKAAARDFLLAIKALFPRIEIPAFWDDDEGIELIKHLSW
                     YISATVVLADWTGSSTRFFPRVAQAMDIKHYWQKALVQAQNALTVFPPKAETAPFTGI
                     NTLFPFIENPTPLQQKVLDLDISQPGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQ
                     GLFFGLPTMATANAMYDRLVKTWLSFYSPESRPSLVLAHSARTLMDRFNESLWSGDLI
                     GSEEPDEQAFSQGCAAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNK
                     ILLADEIHACDAYMSCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGAEGQ
                     QEAPLLGKDDYPWLTHVTKTDVHSHRVATRKEVERSVSVGWLHSEQECIARIESAVSQ
                     GKCIAWIRNSVDDAIQVYRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKY
                     CSLQRASQVIVCTQVIEQSVDIDLDEMISDLAPIDLLIQRAGRLQRHIRDINGQLKRD
                     GKDERSPPELLILAPVWDDAPGDEWFGSAMRNSAYVYPDHGRIWLTQRVLREQGAIQM
                     PHAARLLIESVYGEDVVMPEGFARSEQEQVGKYYCDRARAKKYVLNFRPGYAANINDY
                     LPEKLSTRLAEESVSLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLL
                     EGDAFRQWCVEQRQDPEMANVILVTDDESCGYSAREGLIGKVG"
     gene            complement(93219..93371)
                     /locus_tag="ACHKW0_000099"
     CDS             complement(93219..93371)
                     /locus_tag="ACHKW0_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7472996.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(93631..94365)
                     /gene="cysH"
                     /locus_tag="ACHKW0_000100"
     CDS             complement(93631..94365)
                     /gene="cysH"
                     /locus_tag="ACHKW0_000100"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7472997.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNEINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(94439..96151)
                     /gene="cysI"
                     /locus_tag="ACHKW0_000101"
     CDS             complement(94439..96151)
                     /gene="cysI"
                     /locus_tag="ACHKW0_000101"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HFO7472998.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(96151..97950)
                     /gene="cysJ"
                     /locus_tag="ACHKW0_000102"
     CDS             complement(96151..97950)
                     /gene="cysJ"
                     /locus_tag="ACHKW0_000102"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HFO7472999.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHSSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKD
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            98269..98631
                     /gene="queD"
                     /locus_tag="ACHKW0_000103"
     CDS             98269..98631
                     /gene="queD"
                     /locus_tag="ACHKW0_000103"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO7473000.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            98709..99980
                     /gene="ygcN"
                     /locus_tag="ACHKW0_000104"
     CDS             98709..99980
                     /gene="ygcN"
                     /locus_tag="ACHKW0_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7473001.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEEEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLEPSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            99971..100231
                     /gene="ygcO"
                     /locus_tag="ACHKW0_000105"
     CDS             99971..100231
                     /gene="ygcO"
                     /locus_tag="ACHKW0_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7473002.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            100248..100823
                     /gene="ygcP"
                     /locus_tag="ACHKW0_000106"
     CDS             100248..100823
                     /gene="ygcP"
                     /locus_tag="ACHKW0_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HFO7473003.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(100971..101831)
                     /gene="ygcQ"
                     /locus_tag="ACHKW0_000107"
     CDS             complement(100971..101831)
                     /gene="ygcQ"
                     /locus_tag="ACHKW0_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7473004.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(101828..102607)
                     /gene="ygcR"
                     /locus_tag="ACHKW0_000108"
     CDS             complement(101828..102607)
                     /gene="ygcR"
                     /locus_tag="ACHKW0_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HFO7473005.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(102585..103994)
                     /gene="ygcS"
                     /locus_tag="ACHKW0_000109"
     CDS             complement(102585..103994)
                     /gene="ygcS"
                     /locus_tag="ACHKW0_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7473006.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGISLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(104016..105470)
                     /gene="ygcU"
                     /locus_tag="ACHKW0_000110"
     CDS             complement(104016..105470)
                     /gene="ygcU"
                     /locus_tag="ACHKW0_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HFO7473007.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(105540..106325)
                     /gene="ygcW"
                     /locus_tag="ACHKW0_000111"
     CDS             complement(105540..106325)
                     /gene="ygcW"
                     /locus_tag="ACHKW0_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7473008.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            106644..107921
                     /gene="yqcE"
                     /locus_tag="ACHKW0_000112"
     CDS             106644..107921
                     /gene="yqcE"
                     /locus_tag="ACHKW0_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HFO7473009.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            107948..109426
                     /gene="ygcE"
                     /locus_tag="ACHKW0_000113"
     CDS             107948..109426
                     /gene="ygcE"
                     /locus_tag="ACHKW0_000113"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HFO7473010.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWTWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCNGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT        26022 a        29515 c        27951 g        26338 t
ORIGIN      
        1 aatttaatct atttacagtc ctgtgcgaga ttatttcagc tgttattttc tgaaactctc
       61 agaataattt caagattata tattagccat tttgatgagt aatttagatt attggtgaac
      121 agaacggcgc tctctgctcg ctaattccgc aagcctgaca aaaccgaagt gaataaaaat
      181 gcaatataac agaataaaag aaaacacccc tcactggtgc tttctgctgt taatgtacat
      241 tttctggcat aaaaaatact gtaaatgaca cggtcacgac atacaccgaa ttctctacgc
      301 ttatagcgtt acctcacccc caaactttca ttagtgaaat taaaggaaag gtcatatcta
      361 attgatttaa ttgaaaataa atcatatcgg catgtaaaaa tccacaatgt acaaaaaacg
      421 agccgttacg gaatatttta tctacaaaaa ctgactaaat aaaaattttt cactaattga
      481 atagtcatag ccagcgatat acgctatgcg aaaatgcaga tggcaatgag atccactgct
      541 ttcatctcca ttaacatccc attacgcttt tattaaggag cattagcatg ttctcaccgc
      601 agtcacgctt gcgtcatgca gttgcagata cgttcgcgat ggttgtttac tgttctgtcg
      661 tgaacatgtg tattgaagtt ttcctctccg gaatgagctt cgaacagtct ttttattcca
      721 gattggtagc gattccggtg aacatcttaa ttgcatggcc atacggtatg taccgcgatc
      781 tgtttatgcg cgcggcacgc aaagttagcc cgtcgggctg gataaaaaat ctggctgata
      841 tcctggctta tgtgacgttc cagtcaccgg tgtatgtggc gatcttgtta gtggtgggcg
      901 cagactggca tcagattatg gcggcggtca gttcaaacat cgttgtttcg atgttgatgg
      961 gggcggttta tggctacttc ctcgattatt gccgccgact gtttaaagtc agccgttacc
     1021 agcaggtaaa agcctgactc ttcttttcgc gactggcatc gctagtcgcg tctttaactg
     1081 gcttcgccaa ataaaccatt caaataacgt tcaagcgcaa cacgcgaact aaagccatgc
     1141 gggataccgt aatgctcgtg tcgctcgcct gctaaataga gcggaaattg ctggtaatga
     1201 tcgcaagttt taatatcgga agcgggttca gccagtgttg aactgcgttc ttttaatgtt
     1261 ggatgaatta ccagagcggt acgtcccatt cgcgcttcgc gattaacata gacataattt
     1321 tccccacggc ggtaaccata cgctttctgg gttaccacat cgacagtaaa accgactttt
     1381 tcaagtacgc gcgccacctc gtctggtcgt aaatacatat ttgatcctcg ttattatcaa
     1441 ccgcgggcct accttacctg attgcgcatt cacaacgctt tcagaaaagt ccataaagcc
     1501 gcgatgaacc tcagttaaga aaattatggt ctacactgaa aattacatcg aattctaatg
     1561 ggggatcata tgtttaaccg accgaaccgc aacgacgttg atgatggcgt gcaggatatc
     1621 cagaatgatg tcaatcaatt agctgacagc ctcgaatctg tattgaagtc ctggggcagc
     1681 gacgccaaag gggaagctga agccgcacgc agtaaagctc aggcattgct gaaagaaacc
     1741 agggcgcgaa tgcatggtcg tactcgcgtc cagcaagccg cacgcgatgc cgttggctgc
     1801 gcggattctt ttgttcgtga aagaccctgg tgtagcgtgg gtacagcagc tgcggtaggt
     1861 atttttatcg gcgcactgtt aagcatgcgc aaatcgtagt gcaaaaatga taataaatac
     1921 gcgtctttga ccccgaagcc tggcttcggg gtttcttttt gcctggtgaa tcacaaaaat
     1981 ccccctaccc cgtcacgctc atatccaggg taatttcgac cactatttgc tatatattgt
     2041 gtggttgaat cttttttcaa ctacatctag tatctctgta tcaacagaga tacaacccga
     2101 cgcgtatcat cgcgccgtat cttcatttta aacggaaata cgaatcatgc gcattactat
     2161 ttacactcgt aacgattgcg ttcagtgcca cgccaccaaa cgggcgatgg aaaaccgggg
     2221 ctttgatttt gaaatgatta atgtcgatcg cgttcctgaa gcagcggaag cgttgcgtgc
     2281 tcagggcttt cgtcagttgc cggtagtgat tgctggcgat cttagctggt ctggtttccg
     2341 tccggacatg attaaccgtc tgcatccagc gccacacgcg gccagtgcat gagccagctc
     2401 gtctacttct ccagcaactc cgaaaacacg cagcgtttta tcgaacgttt aggtctgccc
     2461 gcggtgcgca tcccgctcaa tgagcgggaa cggattcagg tagacgagcc ttacatcctg
     2521 atcgtgccct cttacggcgg cggcggtacg gctggcgcgg tgccacgaca ggtaattcgc
     2581 tttttaaacg acgagcacaa ccgggcgttg cttcgcggcg ttattgcttc tggtaatcgc
     2641 aactttggtg aggcgtatgg ccgcgccgga gatgtgattg cccggaaatg cggcgtgccg
     2701 tggctgtacc gttttgaact catgggtacg caaagcgata tcgaaaacgt tcgtaaagga
     2761 gtaaccgaat tttggcaacg acaaccgcag aatgcctgac gcaggaaacg atggattacc
     2821 acgcgctgaa tgcgatgctt aacctctacg atagcgcagg tcgcattcag ttcgataaag
     2881 accgccaggc cgttgacgcc tttattgcga cgcatgtgcg tccgaacagt gtgaccttca
     2941 gtagccagca gcagcgcctg aactggctgg tcaacgaagg ttactatgat gaaagcgttc
     3001 ttaatcgcta ctctcgcgac tttgtcatta cgctgtttgc ccacgcacac accagcggtt
     3061 ttcgtttcca gacattcctc ggggcatgga agttttacac cagctatacg ttgaagacat
     3121 tcgacggtaa acgttatctg gaagattttg ccgatcgagt cacgatggtg gcgctgacgc
     3181 tggcacaagg cgatgcgacg ctggcgttgc aactgaccga tgaaatgctg tcaggacgct
     3241 ttcagccagc cacgccaaca ttcctcaact gcggtaagca gcagcgcggc gaactggttt
     3301 cctgttttct gctgcgtatt gaagacaata tggagtcgat tggccgggcg gtaaattccg
     3361 cactgcaact gtcgaaacgc ggcggcggcg tggcattttt gctgtcgaat ctgcgggaag
     3421 cgggcgcgcc aattaaacgt attgaaaatc aatcttctgg cgtgattccg gtgatgaaaa
     3481 tgctggaaga cgcattttcc tatgccaacc agctcggcgc tcgtcagggg gctggtgcag
     3541 tctatttaca tgcccatcat cccgatattc tgcgttttct cgatacgaaa cgggaaaatg
     3601 ccgacgagaa aatccgcatt aaaacactgt cgctgggcgt ggtgatcccg gatatcactt
     3661 tccatctggc aaaagagaat gcgcagatgg cgctgttttc gccttatgac gtagagcgag
     3721 tttatggcaa gccgtttgcc gatgtggcca tcagccaaca ctatgacgaa ctggttgccg
     3781 atgaacgcat tcgcaaaaaa tacctcaacg cccgtgattt cttccagcga ctggcagaaa
     3841 tccagtttga gtccggctat ccctacatca tgtatgaaga cacggtaaac cgtgctaacc
     3901 ctatcgccgg gcgcataaat atgagtaatc tctgctcaga aattttgcag gttaacagcg
     3961 cctcagagta tgacgagaat ctcgactatg cccgtacagg ccatgatatt tcctgcaatt
     4021 taggttcgtt gaatattgcc cacaccatgg attcccccaa ctttgcccgc acggtagaga
     4081 ctgccgtacg cggtttaacg gcggtgtcag atatgagtca tatccgcagc gtgccgtcca
     4141 tcgaagccgg aaatgccgcc tcacacgcca tcgggctggg gcagatgaat ttacacggtt
     4201 atctggcgcg agaaggcatc gcttatggtt cgccggaagc actggatttc accaatctct
     4261 atttctatac catcacctgg cacgcactgc gtacctcgat gttgctggca cgcgaacgcg
     4321 gtgaaacctt cgccgggttc aaacagtcac gttatgccag tggtgaatat ttcagccaat
     4381 atctgcaagg taactggcag ccgaaaacgg cgaaagttgg cgaactgttt gcccgcagcg
     4441 gtattacgtt acctacccgt gagatgtggg cgcaactgcg cgacgacgtg atgcgctacg
     4501 gcatttataa ccagaatctt caggcggtgc cgccaaccgg ttctatctct tatatcaacc
     4561 atgctacgtc gagtattcat ccgattgtgg cgaaagtaga gatacgcaaa gagggcaaaa
     4621 caggacgcgt ttactaccct gccccgttta tgactaacga gaatctggcg ctgtatcagg
     4681 atgcttacga aatcggcgca gaaaagatca tcgacaccta cgcggaagcg actcgccatg
     4741 tcgatcaggg tttgtcgctg acgctgtttt tccccgatac cgccaccact cgcgatatca
     4801 acaaagcgca gatttacgcc tggcgcaaag gtatcaaaac gctctattac attcgcctgc
     4861 gtcagatggc gctggaaggc actgaaattg aaggctgcgt ctcctgtgca ctttaaggaa
     4921 tatctatgaa actctcacgt atcagcgcca tcaactggaa caagatatct gacgataaag
     4981 atctggaggt gtggaatcgc ctgaccagca atttctggct accagaaaag gtgccgctgt
     5041 cgaacgatat tcctgcctgg cagacattaa ctgtcgtaga acaacaactg acgatgcgcg
     5101 tttttactgg cctgacgctg ctcgacacgc tgcaaaatgt tatcggcgcg ccttcgctga
     5161 tgcccgatgc actcacgcct catgaagaag cggtattatc gaatatcagc tttatggaag
     5221 cggttcatgc ccgctcttac agttcgattt tctcgacgct gtgtcagacc aaagatgtcg
     5281 atgccgccta cgcctggagt gaagaaaacg caccgttgca gcgaaaagct cagattattc
     5341 agcaacatta tcgcggtgat gatccgctga aaaagaaaat cgccagtgtg tttcttgaat
     5401 cttttttgtt ctattccggt ttctggctgc cgatgtattt ttccagccgc ggaaagctga
     5461 ccaataccgc agatctgatc cgtctgatta tccgcgatga agcagtccac ggttactaca
     5521 taggctataa atatcagaaa aacatggaaa agatatctct gggacaacgt gaagagttga
     5581 agagtttcgc cttcgattta ttgctggaac tctacgacaa cgagttgcaa tacaccgatg
     5641 agctgtacgc cgaaaccccg tgggctgacg atgtgaaagc gtttctctgt tacaacgcca
     5701 ataaggcttt gatgaatctg ggctacgaac cgttatttcc cgcagaaatg gcggaagtga
     5761 atccggcaat cctcgccgcg ctttcgccga atgccgatga aaatcacgat ttcttttccg
     5821 gttcaggctc ctcttatgtg atggggaaag cggttgaaac agaagatgaa gactggaatt
     5881 tctgagggtg ttattttcaa aaatatcact acccgcagca gggaaataat tcccgccaaa
     5941 tagcttttta tcacgcaaat attctgtagt gatctacact gatactctgt tgcattattc
     6001 gcctgaaact acaatattca ggcgtttttt cgctatctat gacaaaaaat atcaactttt
     6061 ctcgatttgc tctcagccct tatatcacgg gaaattccgg cgatttgctc gcatcaatat
     6121 tcatgccaca tttgccctca ggggttgcct cagattctca gtatgttagg gtagaaaaaa
     6181 gtgactattt ccattgggta atatatcgac ataaacaaat aaaggaatct ttctattgca
     6241 tggcaattaa attagaaatt aaaaatcttt ataaaatatt tggtgagcat ccacagcgag
     6301 cgttcaaata tatcgaacaa ggactttcga aagaacaaat tctggaaaaa actgggctat
     6361 cgcttggcgt aaaagacgcc agtctggcca ttgaagaagg cgagatattt gtcatcatgg
     6421 gattatccgg ctcgggtaaa tccacaatgg tacgccttct caatcgcctg attgaaccca
     6481 cccgcgggca agtgctgatt gatggtgtgg atattgccaa aatatccgat gccgaactcc
     6541 gtgaggtgcg cagaaaaaag attgcgatgg tcttccagtc ctttgcctta atgccgcata
     6601 tgaccgtgct ggacaatact gcgttcggta tggaattggc cggaattaat gccgaagaac
     6661 gccgggaaaa agcccttgat gcactgcgtc aggtcgggct ggaaaattat gcccacagct
     6721 acccggatga actctctggc gggatgcgtc aacgtgtggg attagcccgc gcgttagcga
     6781 ttaatccgga tatattatta atggacgaag ccttctcggc gctcgatcca ttaattcgca
     6841 ccgagatgca ggatgagctg gtaaaattac aggcgaaaca tcagcgcacc attgtcttta
     6901 tttcccacga tctcgatgaa gctatgcgta ttggcgaccg aattgccatt atgcaaaatg
     6961 gtgaagtggt acaggtcggc acaccggatg aaattctcaa taatccggcg aatgattatg
     7021 tccgtacctt cttccgtggc gttgatatta gtcaggtatt cagtgcgaaa gatattgccc
     7081 gccggacacc gaatggctta attcgtaaaa cccctggctt cggcccacgt tcggcactga
     7141 aattattgca ggatgaagat cgcgaatatg gctacgttat cgaacgcggt aataagtttg
     7201 tcggcgcagt ctccatcgat tcgcttaaaa ccgcgttaac gcagcagcaa ggtcttgatg
     7261 cggcgctgat tgatgcgccg ttagcagtcg atgcacaaac gcctcttagc gagttgctct
     7321 ctcatgtcgg acaggcaccc tgtgcggtgc ccgtggtcga cgaggaccaa cagtatgtcg
     7381 gcatcatttc gaaaggaatg ctgctgcgcg ctttagatcg tgagggggta aataatggct
     7441 gatcaaaata atccgtggga taccacgcca gcggcggaca gtgccgcgca atccgcagac
     7501 gcctggggta caccggcgac tgcaccgact gacggcggtg gtgctgactg gctgaccagt
     7561 acgcctgcgc caaacgtcga gcattttaat attctcgatc cgttccataa aacgctgatc
     7621 ccgctcgaca gttgggtcac tgaagggatc gactgggttg ttacccattt ccgtcccgtc
     7681 ttccagggcg tgcgcgttcc ggttgattat atcctcaacg gtttccagca gttgctgctg
     7741 ggtatgcccg caccggtggc gattatcgtt ttcgctctca tcgcctggca gatttccggg
     7801 gtcggaatgg gcgtggcgac gctggtttcg ctgattgcca tcggcgcaat cggtgcctgg
     7861 tcgcaggcaa tggttactct ggcgctggta ttaaccgccc tgctgttctg tatcgtcatc
     7921 ggtttgccgt tggggatctg gctggcgaga agtccgcgag cggcgaaaat tattcgtcca
     7981 ctgcttgatg ccatgcagac cacgccagcg tttgtttatc tggtgccaat cgtcatgcta
     8041 tttggtatcg gtaacgtgcc gggcgtggtg gtgacgatca tctttgctct gccgccgatt
     8101 atccgtctga ccattctggg gattaaccag gttccggcgg atctgattga agcctcgcgc
     8161 tcattcggtg ccagcccgcg ccagatgctg ttcaaagttc agttaccgct ggcgatgccg
     8221 accattatgg cgggcgttaa ccagacgctg atgctggccc tttctatggt ggtcattgcc
     8281 tcgatgattg ccgtcggcgg gctgggtcag atggtacttc gcggtatcgg tcgtctggat
     8341 atggggcttg ccaccgttgg cggcgtcggg attgtgatcc tcgccattat cctcgatcgc
     8401 ctgacgcagg ccgttgggcg cgactcacgc agtcgcggca accgtcgctg gtacaccact
     8461 ggccctgttg gtctgctgac ccgcccattc attaagtaac tctgcacttg cccggtgacg
     8521 ccgggcatta tcaccctgcc aaaaaaagga ataacaatgc gacatagcgt actttttgcg
     8581 acagcgtttg ccacgcttat ctctacacaa acttttgctg ccgatctgcc gggcaaaggt
     8641 attactgtta atccagttca aagcaccatc accgaagaaa ccttccagac gctgctggtc
     8701 agtcgtgcgc tggagaaatt aggttatacc gtcaacaaac ccagcgaagt ggattacaac
     8761 gttggctaca cctcgcttgc ttccggcgat gcaaccttca ccgccgtgaa ctggacgcca
     8821 ctgcatgaca acatgtacga agctgccggt ggcgataaga aattttatcg tgaaggggta
     8881 tttgttaacg gcgcggcaca gggttacctg atcgataaga aaaccgccga ccagtacaaa
     8941 atcaccaaca tcgcacaact gaaagatccg aagatcgcca aactgttcga taccaacggc
     9001 gacggaaaag cggatttaac cggttgtaac cctggctggg gctgcgaagg tgcgatcaac
     9061 caccagcttg ccgcgtatga actgacccat accgtgacgc ataatcaggg gaactacgcg
     9121 gcgatgatgg ccgacaccat cagtcgctac aaagagggca aaccggtgtt ttactacacc
     9181 tggacgccgt actgggtgag taacgaactg aagccgggca aagatgtcgt ctggttgcag
     9241 gtgccgttct ccgcactgcc gggcgataaa aacgccgata ccaaactgcc gaatggtgcg
     9301 aattatggct tcccggtcag caccatgcat atcgttgcca acaaagcctg ggccgagaaa
     9361 aacccggcag cagcgaaact gtttgccatt atgcagttgc cagtggcaga tattaacgcc
     9421 cagaacgcca ttatgcatga cggcaaagcc tcagaaggcg atattcaggg ccacgttgat
     9481 ggctggatca aagcccacca gcagcagttc gatggctggg tgaatgaggc gctggcagcg
     9541 cagaagtaat ttttattcag gcggataagg cgtttttgcc tcatccgccg ctctgtacac
     9601 aatgcctgat gcgacgctga cgcgtcttat catgcctaca aaatacactc attccgtatg
     9661 gcggataagg cgttttcgcc gcatccgccg ttctgtgcac aatgcctgat gcgacgctgg
     9721 cgcgtcttat catgcctaca aaccacatcg cacatttcaa caagctatct ttcatcccat
     9781 attcatcaac atccgctatt attgatttcc agcttaatca tcacctgatg aacaaaaata
     9841 atgactaaac ctaatcatga gcttagcccg gcgctgatcg tgctgatgtc tatcgccacc
     9901 ggtctggcgg tcgccagtaa ctattacgct cagccattgc tcgacaccat cgcgcgtaac
     9961 ttttcccttt ccgccagttc ggcaggcttt attgttaccg ccgcgcaatt gggctatgca
    10021 gcaggtctgc tgtttcttgt tcccctcggt gatatgtttg aacgccgccg cctgattgtc
    10081 tcgatgacct tactggcggc aggcggcatg ttaatcactg ccagcagtca gtcgctggcg
    10141 atgatgatcc tcggtacggc attaaccggt ttattctctg tcgtggcaca aattctggtt
    10201 ccgctggcag cgacgctggc ttcaccggac aaacgcggca aagtggttgg cactattatg
    10261 agcggtctgc tgttggggat cttgctggca cggacagttg ccggattgct ggcgaatctc
    10321 ggcggctggc gcaccgtctt ttgggttgct tcggtgttaa tggcactgat ggcgctggca
    10381 ttatggcgtg gtctgccaca aatgaaatca gaaacccacc tcaactaccc acagttgttg
    10441 ggttccgttt tcagtatgtt tatcagcgat aaaattctgc gtacccgcgc gttgctgggc
    10501 tgcctgacct ttgccaattt cagcattctc tggacctcaa tggccttttt gcttgccgct
    10561 ccacctttta actacagcga tggtgtaatt ggtctgtttg gacttgcggg agctgccgga
    10621 gcgttgggcg ctcgtccggc gggcggtttt gccgataagg gcaaatcgca ccacaccaca
    10681 actttcggtc tgctgctgct attactttca tggctggcga tctggtttgg tcacacttcc
    10741 gtactggcgt tgattatcgg aatcctggtg ctggatctca ccgtgcaggg cgtgcatatc
    10801 actaaccaga cggtaattta tcggatacat cctgatgcgc gcaatcgcct gaccgcaggt
    10861 tacatgacta gctactttat tggcggtgcc gccggttcgc taatttcagc ctcagcctgg
    10921 caacatggcg gttgggctgg cgtttgtctg gctggcgcga cgattgccct ggttaactta
    10981 ctggtctggt ggcgaggttt tcatcgtcag gaagccgcaa attaagcaat cagcaaccgt
    11041 tttcgtggct ttacacttat aagggtgtta agaagcccat cagtctgata aggttaagat
    11101 attcattcag tctatttata atattaacaa tcgttaagcg tacactctat ggaaagccct
    11161 actccacagc ctgctcctgg ttcggcgacc ttcatggaag gatgcaaaga cagtttaccg
    11221 attgttatta gttatattcc ggtggccttt gcgttcggtc tgaatgcgac ccgtctggga
    11281 ttctctcctc tcgaaagcgt ttttttctcc tgcatcattt atgcaggcgc gagccagttc
    11341 gtcattaccg cgatgctggc agccgggagt agtttgtgga ttgctgcact gaccgtcatg
    11401 gcaatggatg ttcgccatgt gttgtatggc ccgtcactgc gtagccgtat tattcagcgt
    11461 ctgcaaaaat cgaaaaccgc cctgtgggcg tttggcctga cggatgaggt ttttgccgcc
    11521 gcaaccgcaa aactggtacg caataatcgc cgctggagcg agaactggat gatcggcatt
    11581 gccttcagtt catggtcatc gtgggtattt ggtacggtaa taggggcatt ctccggcagc
    11641 ggcttgctgc aaggttatcc cgccgttgaa gctgcattag gttttatgct tccggcactc
    11701 tttatgagtt tcctgctcgc ctctttccag cgcaaacaat ctctttgcgt taccgcagcg
    11761 ttagttggtg cccttgcagg cgtaacgcta ttttctattc ccgtcgccat tctggcaggc
    11821 attgtctgtg gctgcctcac tgcgttaatc caggcattct ggcaaggagc gcccgatgag
    11881 ctatgaggtt ctactgcttg ggttactggt tggcgcggcg aattattgct tccgctattt
    11941 gccgctgcgc ctgcgtgtgg gtaatgcccg cccaaccaaa cgtggcgcgg taggtatttt
    12001 gctcggcacc attggcatcg cctcgatatg cgctctgctg gttgtctcta ccgcaccaga
    12061 agtaatgcac gatacacgcc gtttcgtgcc cacgctggtc ggcttcgcgg tactgggtgc
    12121 cagtttctat aaaacacgca gcattatcat cccaacactg cttagtgcgc tggcctatgg
    12181 gctcgcctgg aaagtgatgg cgattatata acccacaaga atcatttttc taaaacaata
    12241 catttacttt atttgtcact gtcgttacta tatcggctga aattaatgag gtcataccca
    12301 aatggatagt tcgtttacgc ccattgaaca aatgctaaaa tttcgcgcca gccgccacga
    12361 agattttcct tatcaggaga tccttctgac tcgtctttgc atgcacatgc aaagcaagct
    12421 gctggagaac cgcaataaaa tgctgaaggc tcaggggatt aacgagacgt tgtttatggc
    12481 gttgattacg ctggagtctc aggaaaacca cagtattcag ccttctgaat taagttgtgc
    12541 tcttgggtca tcccgtacca acgcgacgcg tattgccgat gaactggaaa aacgcggttg
    12601 gatcgaacgt cgtgaaagcg ataacgatcg ccgctgcctg catctgcaat taacggaaaa
    12661 aggtcacgag tttttgcgcg aggttttacc accgcagcat aactgcctgc atctactctg
    12721 gtccgcgctc agcacaacag aaaaagatca gctcgagcaa atcacccgca aattgctctc
    12781 ccgtctcgac cagatggaac aagacggtgt ggttctcgaa gcgatgagct aacgcgtcat
    12841 ctcgctcaaa aatccagact tataaaagaa aaaatgactg gccagcatcg caacatgctg
    12901 gcctttttgg caagcaggtc ggctcagccg atgagttaag aagatcgtgg agaacaatat
    12961 gagcgcaaat gcggagactc aaaccccgca gcaaccggta aagaagagcg gcaaacgtaa
    13021 gcgtctgctc ctccttctca ccttgctctt tataattatt gccgtagcga tagggattta
    13081 ttggtttttg gtactgcgtc atttcgaaga aaccgatgac gcatacgtgg cagggaatca
    13141 aattcaaatt atgtctcagg tgtctggcag cgtgacgaaa gtctgggccg ataacaccga
    13201 ttttgtaaaa gaaggcgacg tgctggtcac tctcgacccg acagatgctc gccaggcgtt
    13261 tgaaaaagcc aaaactgcac tggcttccag cgttcgccaa acccaccagc tgatgattaa
    13321 cagcaaacag ttgcaggcga atattgaggt gcagaaaatc gccctcgcaa aagcacaaag
    13381 cgactacaac cgccgtgtgc cgctgggcaa tgccaacctg attggtcgcg aagagctgca
    13441 acacgcccgc gacgccgtca ccagtgccca ggcgcaactg gacgtcgcta ttcaacaata
    13501 caatgccaat caggcgatga ttctggggac taaactggaa gatcagcctg ccgtgcaaca
    13561 ggctgccacc gaagtacgta acgcctggct ggcgctggag cgtacccgta ttgtcagtcc
    13621 gatgaccggt tatgtctccc gccgcgcggt acagcctggg gcgcaaatta gcccaacgac
    13681 gccgctgatg gcggtcgttc cagccaccaa tatgtgggtg gatgccaact ttaaagagac
    13741 gcagattgcc aatatgcgta tcggtcagcc ggtcactatc accacggata tttacggcga
    13801 tgatgtgaaa tacaccggta aagtggttgg tctggatatg ggcacaggta gcgcattctc
    13861 actgcttccg gcgcaaaatg cgaccggtaa ctggatcaaa gtcgttcagc gtctacctgt
    13921 gcgtatcgaa ctggaccaga aacagctgga gcaatacccg ctgcgcattg gtttgtccac
    13981 gctggtgagc gtcaatacca ctaaccgtga cggtcaggta ctggcaaata aagtacgttc
    14041 aacaccggtc gcggtaagta ccgcgcgtga aatcagccta gcacctgtca ataaactgat
    14101 cgacgatatc gtaaaagcta acgctggcta atccagaggt gcgtgtgatg caacagcaaa
    14161 aaccgctgga aggcgcgcaa ctggtcatta tgacgattgc gctgtcactg gcgacattca
    14221 tgcaggtgct ggactccacc attgctaacg tggcgatccc cactatcgcc gggaatctgg
    14281 gctcatcgct cagccaggga acgtgggtaa tcacttcttt cggggtggcg aatgccatct
    14341 cgatcccgct taccggctgg ctggcaaagc gcgtcgggga agtgaaactg ttcctttggt
    14401 ccaccatcgc ctttgctatt gcgtcgtggg cgtgtggtgt ctccagcagc ctgaatatgc
    14461 tgatcttctt ccgcgtgatt caggggattg tcgccgggcc gttgatcccg ctttcgcaaa
    14521 gtctattgct gaataactac ccgccagcca aacgctcgat cgcactggcg ttgtggtcga
    14581 tgacggtgat tgtcgcgcca atttgcggcc cgatcctcgg cggttatatc agcgataatt
    14641 accactgggg ctggatattc ttcatcaacg tgccgattgg cgtggcggtg gtgttgatga
    14701 cactgcaaac tctgcgcgga cgtgaaaccc gcaccgaacg gcggcggatt gatgccgtgg
    14761 ggctggcact gctggttatt ggtatcggca gcctgcagat tatgctcgac cgcggtaaag
    14821 agctggactg gttttcatca caggaaatta tcattcttac cgtggtggcg gtggtggcta
    14881 tctgcttcct gattgtctgg gagctgaccg acgataaccc gatagtcgat ctgtcgttgt
    14941 ttaagtcgcg caacttcacc atcggctgct tgtgtatcag cctcgcgtat atgctctact
    15001 tcggcgctat tgttctgctg ccgcagttgt tgcaggaggt ctacggttac acggcgacct
    15061 gggcaggttt ggcctctgcg ccggtaggga ttattccggt gatcctgtcg ccgattatcg
    15121 gccgcttcgc gcataaactg gatatgcggc ggctggtaac cttcagcttt attatgtatg
    15181 ccgtctgctt ctactggcgt gcctatacct ttgaaccagg tatggatttt ggcgcgtcgg
    15241 cctggccgca gtttatccag gggtttgcgg tggcctgctt ctttatgccg ctgaccacca
    15301 ttacgctgtc tggtttgcca ccggaacgac tggcggcggc atcgagcctc tccaacttta
    15361 cgcgtacgct ggcggggtct atcggcacgt ccatcaccac caccatgtgg accaaccgcg
    15421 agtcgatgca ccacgcacag ttaaccgagt cggtaaaccc gtttaaccca aacgcccagg
    15481 cgatgtacag tcaactggaa gggcttggga tgacgcaaca gcaggcctcc ggctggattg
    15541 cccagcagat caccaaccag gggctgatta tttccgccaa tgagatcttc tggatgtcag
    15601 ccgggatatt cctcgtcctg ctggggctgg tgtggtttgc taaaccgcca tttggcgcag
    15661 gtggcggcgg aggcggtgcg cactaagtac aactaagcca gttcatttga actggctttt
    15721 ttcaattaat tgtgaagata gtttactgac taaatgtgca gttcctgcaa cttctcttcc
    15781 ggcagcgcca gttcttcgtt gctgttgatg cgcacgtcac gttccagaat gctacgcgca
    15841 atatcctgcg cttcctgcaa cgagtgcatc tggtaagtgc cacactggta gacgttcagt
    15901 tccgggatct gattctgatc ctgcactttc agcacgtctt ccattgccgc tttccaggca
    15961 tcagcaacac gctgctcatc tggcgtacca atcagactca tataaaaacc ggtgcggcag
    16021 cccattggcg agatatcgat aatctctaca ccattaccgt taagatggtt acgcataaaa
    16081 ccagcaaaca ggtgctccag ggtatggatc cctctttctg gcatcacttc tttgttcggc
    16141 acgcagaagc gcagatcgaa cacggtgatt gcgtcgccat gcggggtgtt cattgttttc
    16201 gccacccgaa ctgcaggcgc ttccatccgg gtatgatcga ctgtgaagct atctaacaac
    16261 ggcatttagc cacctccggt aattttttta aaaattttct gaactctttc ttcccaggcg
    16321 agtctgagta tatgaaagac gcgcatttgt tatcatcatc cctgaattca gagatgaaat
    16381 tttggccact cacgagtggc ctttttcttt tctgtcaggc gtgtttttcc agccacaccg
    16441 caaacggttc ggtatcagcg gcttccattt cctgctgacg gcgttcagac gcctcgcgct
    16501 cggctacaaa atcctcttcg cgcagaattt ccagcggctc ttcacgcagc agattacggt
    16561 aggcttctgc aaatgctttg cctgttccgc caataccagt atcaatcata gaccttaaga
    16621 tacgggcaga gaaagtcaga tcgggattat cgaagcaggc aaccagttca tcacacactt
    16681 tctgatacgc ttcgccgccg ttaatactat ccagcgtttg cgcgacgcgt ttcagatcgc
    16741 ggaacagatc tttacccacc tgcggtaacg ggaactgtgc ggtttcgcag ccgataccca
    16801 gcgtcagacc cggtttgcga ccttcgagga tcacccggtt ccagttaacg cgtgtacagg
    16861 caagttcgct actgctcatt tccggtgcat cagccagcgc acaccagacc ataaacaggt
    16921 cgaggaatcg cacctgctgt tcatctacac caatcggcga gaacgggttg atgtccagcg
    16981 aacgcacttc aatatattca atgccgccac gtaacagcgc atcagaaggc gactcgccgc
    17041 tgcgggtaac gcgttttgga cgaatcggcg cgtacagttc gttttcaatc tgcaacacgt
    17101 tgctgttgat ttgcagcctc ttaccgtctt tctcaatacc aatcttcgcg tactcttccg
    17161 atggcgtttt gattgcctgt ttaaggcccg ctacgtactc gtaaagatcg ttgaaggtaa
    17221 taccaagatt gctttgcgat ttattggtat agccgagatc gctcaaacga agagaggtcg
    17281 catacggcag gtaatacata ccgcactcgg ttttctcaaa cggcagcgac gttggttttc
    17341 cttgcaggaa agaagaacaa atcgccggag atgcaccaaa cagataagga atgacccaac
    17401 cgaaacgata gtaattgcgg ataacgcgga aatagcccgc agaaattttc tctttggcat
    17461 cagcgcccga gatatcaccg cacttcgctt gccagaatgc cattggcaaa gagaaattgt
    17521 agtgcacgcc ggaaatggtt tgcatcagcg cgccgtagcg atttttcagc ccttcacgat
    17581 acagcgtttt aaagcgtccg gtgttagaag tgccgtactg tgccagttcg atgtcctgac
    17641 cttctgcgat gtagcatggc atacttaacg gccacatccg ctcatcgccc atattgcgcg
    17701 ccgtataacg atgcagatcg cgcataaagg tcagcatatg ttcaatatca ccatccactg
    17761 gtgtaatgaa ttccagcaat gcttccgcaa aatcggtagt aatccatttg tgcgtcagtg
    17821 cggaacctaa tgcttcagga tgacctgttg ttgccagtgt gccatcagca ttaacacgca
    17881 aagtttcgcg ctccagccca cgctgtatcc cctttaacgc ctgaggatgt ttttccagcc
    17941 aggccagcgc ctgtgatacg tccgggatca aattgacctc ccgcctgtca aaatcgtttt
    18001 aattagcata actgtaatgg tgaccatatg tgcaggccta caattagtgc caccacatca
    18061 tgccctgaac ggtcgctgct gcaactgcaa catagcgtaa cgctttacca aggcacaaaa
    18121 aaaagattac cggtccccac gagatgcgca tccatcccgc taacagacac agtaaatcgc
    18181 caaccacggg catccagctt aataatagcg tgactgcacc atagcgtttc agccagccgg
    18241 tagctttctc ttgccagcgc gatgttttac gcaatggaaa gaaacgccca aggataacgt
    18301 tagttaaccc tccaaggcta ttacccattg ttgctgttaa aactaaaacc cagggatgac
    18361 tgatcccgga aagcaacatt gccaccagca cgacttcgga gttgccgggt aatagtgtag
    18421 cgctgagaaa actactggca aacaacgaga aaagcgataa cgcttcactc acagcaagcg
    18481 aacatccacg gcgtccatgc ctgctgcacg ggccgcctga ataccgaaat cggcatcttc
    18541 aaagaccaca cactgcgtcg gttgcacgcc catacgctgc gcgcacaaca aaaatgtgtc
    18601 tggcgcgggt ttatggtgtt tgacgtgatc agcagcgacg acggcgtcaa aataacggcg
    18661 taatcccagg tgcgccagca atgcctcagc gatggcgctt tcactccccg ttcctacagc
    18721 cattgggcga cgaccatgcc aacttttcac cacttcaaca agaggaagcg gctcgacact
    18781 atccagcagc atacttctta ctgcttctgt tttttcacgc gctaacgcat gcgggtcgag
    18841 atcggcctga ttcagctcaa taattgcctg agcaatacgc caggtgggcg atccattaag
    18901 cgcaatcatt gcctgaacat cgtactgaag accgtagtgc cctaatactt cgcgccacgc
    18961 tttacggtgc gtaggctccg tatccaggat tgtgccatcc atatcaaaaa ttaaacctgc
    19021 ataacgctcg tacatggtct tctcgcaagt cgaaaaatat gacgctactt tagcgtaatt
    19081 gcttgatttt gtcgctgatg gcgaaggaga agaaaattca ggaaatggat aaagtagtta
    19141 tttagaaatg agatattttt gaaggaaatt tttgggagaa gatggtgcat ccgggaggat
    19201 tactcactgg cgttcgccct tcgggccgtt gctgacgcaa cgttatcctc cctggtgcct
    19261 gcggttaata ccgctatctc ttcgatacct tcattgctga aggttacgga gaatatggtg
    19321 catccgggag gattcgaacc tccgaccgct cggttcgtag ccgagtactc tatccagctg
    19381 agctacggat gcatcgggaa aatttatttt actgctgata ttgatatcgc tactaaagcc
    19441 atatcaagta agagatggtg catccgggag gattactcgc cttgcggctc gcccttcggg
    19501 ccgttgctaa agcaacgtta tcatccctgg tgcctgcggt taattccgct acctcttcga
    19561 taccttcatt gctgaagatt tcggagaata tggtgcatcc gggaggattc gaacctccga
    19621 ccgctcggtt cgtagccgag tactctatcc agctgagcta cggatgcatc gggaaaattt
    19681 attttactgc tgatattgat accgctacta aagccatatc aagtaagaga tggtgcatcc
    19741 gggaggattc gaacctccga ccgctcggtt cgtagccgag tactctatcc agctgagcta
    19801 cggatgcatc gggaaactta ctttactgca gattttttga taccgctact aaagccgtat
    19861 caagtaagag atggtgcatc cgggaggatt actcgccttg cggctcgccc ttcgggccgt
    19921 tgctaaagca acgttatcct ccctggtgcc tgcggttata ccgccacctc ttccatacct
    19981 tcatcgctga aagtacgtag aatatggtgc atccgggagg attcgaacct ccgaccgctc
    20041 ggttcgtagc cgagtactct atccagctga gctacggatg caaatggcgg tgaggcgggg
    20101 attcgaaccc cggatgcagc ttttgaccgc atactccctt agcaggggag cgccttcagc
    20161 ctctcggcca cctcaccaca cgcctcttac gagtgcttcg aagaacttgt ttatcgctca
    20221 tcgtcgctgc gtggcgcaca tattactttc tgagacttat aagtcaaaca atttttccta
    20281 cacttttatc gtttgcacac ttcacgttca attagtctgt aaaaacgtca aaaagagtgt
    20341 tttatcaaca gaaaaatgga ggtctgacag atagtagtaa tgcaaaaaaa tggagactta
    20401 agttgaatga acgggagtaa agcgaaaaga ctatagagtg aaggagaaat tttgagggtg
    20461 cgtctcaccg ataaagatga gacgcggaaa gattagtaac tggactgctg ggatttttca
    20521 gcctggatac gctggtagat ctcttcacgg tgaacagaaa cttccttcgg ggcatttacg
    20581 ccaatacgta cctggttgcc ctttacccct aaaactgtca cggtgacctc atccccaatc
    20641 atgagggtct caccaactcg acgagtcaga atcagcattc tttgctcctt gaaagattaa
    20701 aagagtcggg tctctctgta tcccggcatt atccatcata taacgccaaa aagtaagcaa
    20761 tgacaaacac attacatgta agcagtcatg gcattacatt ctgttaaacc taagtttagc
    20821 cgatatacac aacttcaacc tgactttatc gttgtcgata gcgttaatgc gaatgccgtg
    20881 aagcgagtcc acggcattgc ctgacgctta tattattgca atttcgcgct gacccagcct
    20941 ttcacactgg ctaacgctgc aggtaaggcc gcagcatccg taccaccggc ttgcgccatg
    21001 tcaggacgtc caccaccctt gccgcccacc tgctgagcga ccataccaat cagttcccct
    21061 gctttcacac gatctgtgac gtccttagat acgcctgcaa tcagagaaac cttaccttcg
    21121 gctaccgttg ccagcacgat aattgtcgac cccagctgat tttttaaatc gtcaaccatg
    21181 gtacgcaaca ttttcggctc aacaccgcta agctcgctaa ccaacagctt aacaccatta
    21241 acatcaattg ccttactgga aagatttgcg ctctcctgtg cggcagcttg ttctttaagc
    21301 tgttgtagtt ctttttctaa ctgacgcgta cgttccagta ctgagcgcac tttatccgcc
    21361 agattattgc tatcgccttt cagcagatgc gcgacttcgc ttaagcgatc gctgtctgca
    21421 tgaacggtgg tgatagcgcc ttctccggtt accgcttcga tacgacgaac gcctgcagca
    21481 gtacccgatt cagagatgat gcggaacaga ccaatatcac cagtgcggct ggcgtgagta
    21541 ccgccacaca gctcggtgga gaaatcgccc atgctcagca cgcgtacacg ctcatcatac
    21601 ttctcgccga acagcgccat cgcacctttc gctttcgccg cttcgagatc catgatgttg
    21661 gtttcgatcg gcaaattgcg acgaatctgt gcgttcacca ggtcttcgac cgcacgaatc
    21721 tcttccggtt tcatcgcttc gttgtgtgag aagtcgaagc gcagcacctt gtcgttaacc
    21781 agtgaacctt tctgcgatac atgagtcccc agaacctggc gcagcgcagc gtgcatcagg
    21841 tgcgttgcgg agtgattcag acgaatacgg gcgcgacgag cctcatcaac atcagcctgc
    21901 acagcgtcgc ccactttcag agaacccgca gcaagtttac cgatgtgacc aatcgcctgg
    21961 ccgtatttct gcgtatcttc caccgcgaag gagaagttag cgcctttcag ttcgccttta
    22021 tcaccaacct gaccgccgga ttccgcatag aatggtgttt gatccagcac gaccacagct
    22081 tcctggcctg cattgatggc atcaaccgct ttaccatcaa caaacagcgc ggtcactttg
    22141 ccgttcagtt ccagatggtc atagccttta aattcagatg cactatcaac acggatcatt
    22201 gcgttgtaat cggcaccaaa gccgctggct tcgcgcgcgc gacgacgctg ctcttccatt
    22261 gctgcttcaa aaccagcttc gtcaactttg atgttgcgct cacgacaaac atcagccgtc
    22321 aggtcaaccg ggaagccata ggtgtcgtac agacggaaag ccgtttcacc atccagcgta
    22381 tcaccagaaa gttttgccag ctcttcatcc agcaacgcca gaccgcgctc cagagtacgg
    22441 gcaaactgct cttcttcagt cttcagcacc tgctcaacct gcgcctgctg gcgtttcagg
    22501 tcttcacccg cagagcccat aacgtcgatc agcggaccaa ccagtttgta gaagaaggtt
    22561 tctttcgcgc cgagcatatt gccgtgacgc actgcgcgac gaatgatacg acgcagtaca
    22621 taaccacggt tttcattgga cggcattacg ccatccgcga tcaggaacgc acaagaacga
    22681 atgtggtcag cgattacgcg cagcgattta ttgctcagat cggtcgcgcc agtgactttc
    22741 gctaccgcct ggatcaacgt gcggaacagg tcgatgtcat agttagagtt aacgtgttgc
    22801 agcaccgcag caatacgctc cagacccata ccggtatcta cagacggctt cggcagcggt
    22861 tccatcgtgc catctgcctg gcggttgaac tgcatgaaga cgatgttcca gatctcaatg
    22921 tagcggtcgc cgtcttcttc cggacttccc ggagggccac cccaaatgtg gtcgccgtga
    22981 tcgtagaaga tttcggtgca cgggccgcac ggaccagtgt cacccatctg ccagaagttg
    23041 tcagatgcgt atggcgcacc tttgttatcg ccgatgcgaa taatacgttc gcgcgggatc
    23101 ccgacttctt tttcccagat ttcgtaggct tcgtcgtcgc tttcatagac ggtaacccac
    23161 agacgctctt tcggcagggc aaaccatttt tcgctggtca gcagttccca tgcaaactga
    23221 atggcatcgt gtttgaaata gtcgccgaag ctgaagttgc ccagcatttc gaagaaggta
    23281 tggtgacgcg cggtgtaacc gacgttttcc aggtcgttgt gtttaccacc cgcacgcacg
    23341 cagcgttggg aagtggtagc gcgggaataa ttacgcttgt cgagcccaag gaacacatcc
    23401 ttgaactggt tcatcccggc gttggtaaac aacaaagttg ggtcgttatg gggtaccagg
    23461 gagctgctgg caactacctg atgtccctta ctatggaaaa agtcgagaaa cgcctgacgg
    23521 atctcagcgg tgctcttgct cataattatc ctgaaatcaa gctaacgaaa tatcgccacc
    23581 agctccagcg tgtcttaacc gccgggctgg taactgaaaa gtgggaataa gataagtttt
    23641 cttgactggg aagtaaaata ccgtatgcgt tcagtcggca aaatttcgcc aaatatcctg
    23701 gatatcttcc atcagatagc cacgatagag cagaaaacgc tggatcttaa ctttttctga
    23761 aaagacagtt ggcaaaggtt caccatattt tcgcgtcgcc tgatcgcgcg ccagtgcgca
    23821 ccagtcgatg tcacattcac gcatcgcttt ttctgtcgct tcgcgggaaa tacctttctg
    23881 attcagttcc tgacgaatac gtgcaggtcc ataacctttg cggctacggc tggcgataaa
    23941 gcgcgcaaca aatcggctgt catcgagata gccatgttca tggcaccagg caataacgcg
    24001 ctcgtaatct tctgccgtag catcaatctc ttctgggcca tttttgccca taatcggtgc
    24061 cgcgagttta cgtcgcagtt cttgctcact gtgatcgcgc accgccagaa tgcgtaccgc
    24121 acgatccaac aggcgagcat atgccgggcg acgggacgtt gattctgtca tggcatatcc
    24181 ttacaactta aaaaagcaaa agggccgcag atgcgaccca ggttttgctg ataaacagat
    24241 ttaatagtca atgagtgtgc agaatgttcc cgcaggccca attcgttaga ggatcgaggt
    24301 gagagtaatt tttcacgcca gtgctggcct gttcccctca ccctaaccct ctccccaaag
    24361 gggcgagggg accgtccgtg cacacattat ctattatcat caataacgaa cgattaaaaa
    24421 tcttcgttag tttctgctac gccttcgctg tcatctacag agaaatccgg cgttgagttc
    24481 gggttgctca gcagcaactc acgtactttc ttctcgatct ctttcgcggt ttccgggtta
    24541 tctttcagcc aggcagttgc attcgcttta ccctgaccga tcttctcacc tttgtagctg
    24601 taccacgcgc ctgctttctc gatcagcttc tcttttacgc ccaggtcaac cagttcgccg
    24661 tagaagttga taccttcgcc gtagaggatc tggaattcag cctgtttaaa cggcgcagcg
    24721 attttgttct tcaccacttt cacgcgggtt tcgctaccca ccacgttttc gccctctttc
    24781 accgcgccga tacgacggat gtcgagacga acagaggcgt agaatttcag cgcgttacca
    24841 ccggtagtgg tttccgggtt accgaacatc acaccaattt tcatacggat ctggttgatg
    24901 aagatcagca gcgtgttgga ctgcttcagg ttacccgcca gcttacgcat cgcctggctc
    24961 atcatacgtg ccgcaaggcc catgtgagag tcgccgattt cgccttcgat ttccgctttc
    25021 ggcgtcagtg ccgccacgga gtcaacgacg ataacgtcta ctgcgccaga acgcgccagg
    25081 gcgtcacaga tttccagtgc ctgctcgccg gtgtccggct gggagcacag caggttatcg
    25141 atatcgacgc ccagtttacg tgcgtagatt gggtccagcg cgtgttcagc atcgataaac
    25201 gcacaggttt taccttcacg ctgcgctgcg gcgatcacct gcagcgtcag cgtggtttta
    25261 ccggaagatt ccggtccgta gatttcgacg atacggccca tcggcagacc acctgcccca
    25321 agcgcgatat ccagtgaaag cgaaccggta gagatggttt ccacatccat ggaacggtct
    25381 tcacccaggc gcatgatgga gcctttacca aattgtttct caatctggcc cagtgctgcc
    25441 gccaacgctt tctgtttgtt ttcgtcgata gccattttta ctcctgtcat gccgggtaat
    25501 accggatagt caatatgttc tgttgaagca attatactgt atgctcatac agtatcaagt
    25561 gttttgtaga aattgttgcc acaaggtctg caatgcatac gcagtagcct gacgacgcac
    25621 cgcatcacgg tcgccgctga agcattcccg ccgggtaatg ccttcaccgc gggcagtggc
    25681 aaaagcaaac cagacggtgc cgacaggctt ctcttcactg ccgccatccg gcccggcgat
    25741 accactaata gacacggcat aatcagcacg agccgctttc agtgcgccta tcgccatttc
    25801 caccacgacg ggttcactca ccgcgccatg ctgcgccagc gtctcttcgc gtacgccgat
    25861 catctgcgct ttggcttcgt tactgtaggt gacaaatccg cgttcaaacc aggcggagct
    25921 accggcaata tcggtaatca ctttcgctac ccaaccaccg gtacaagact cggcagttgt
    25981 tacggttgcg ccacgggctt tcagcgcctg cccaacctgt tcacttaact gcatcagttc
    26041 actgtcagtc atcacaacct ctgtcagtca aaaatttatg ccggacaaga tagcactttc
    26101 gtcaggaaga tggtgttggc gttgcaattt tacgaggagg attcagaaaa aagctgatta
    26161 gccagaggga agctcacgcc cccctcttgt aaatagctac tgtactcgcg ccagcgccac
    26221 ggcttgtcct aactgccaga ccgccattgc gtaatgggtg ctgcggttgt aacgggtgat
    26281 ggtgtagaag ttcggcagac cgtaccagta ctggtagccg gtgccaacat ccagacgcag
    26341 caggctggct tgttgatggt tgcccagcgg ctgctgtggc gttaaacctg cggcggcaag
    26401 ctgcgaaatg ctgtacttag ttttgaagcc atttggcaag cctggagcct gaccgtttgc
    26461 cattaccgcg acctgatcgc ctttcaccca gccgtgcgct ttgaaatagt tcgccacgct
    26521 accgatcgca tcaaccggat cccacaggtt gatatgcccg tcgccgctga aatctaccgc
    26581 atattgtttg taagacgacg gcataaactg tccgtagccc atcgccccgg caaaggaacc
    26641 tttcagattg agcggatcgt cctgctcgtc gcgcgccatc aacaggaagg tttccagctc
    26701 gccagagaaa tactccgcgc ggcgtgggta gttaaatgac agcgttgcca gcgcatcgag
    26761 gatgcgagtt ttccccatca cgcgccccca gcgggtttca acgccgataa tcccgacgat
    26821 aatttccggc ggtacgccat acacctgcca cgcgcgattc aacgcatctt catactgatt
    26881 ccagaaaacc acaccgttct gcacgttgtc cggcgtaata aattttttgc gataacgcag
    26941 ccatgcgccg ttcggacctg atggcggttt caccgatgtg gttggtgcct ggttatccat
    27001 cagccgcagt accgaatcca gacgtttcgc ctgggagaga atttcctgca actgctgacg
    27061 atcgaaaccg tgtttgttca ccattttgtc gatgaactgc tgggcattcg ggttattagc
    27121 gaaatcgccg cccatctgca tcacattgtg ctgcggctca agcaggaagc cgccagacgg
    27181 cgttccggtg gtcgtatcag tctcagtagg ttttggcttg ctgctacagg cggcaagcaa
    27241 cacaaaaagg ggaagcaatg ttacataacg acgcttgaac atgaggggtc catttaacag
    27301 attcaaccag gggcaagtat ggtaaagcat cacgccccgc acaaggaagc ggtagtcact
    27361 gcccgatacg gactttacat aactcaactc attcccctcg ctatcctttt attcaaactt
    27421 tcaaattaaa atatttatct ttcattttgc gatcaaaata acacttttaa atctttcaat
    27481 ctgattagat taggttgccg tttggtaata aaacaataaa tcctgaagga gagaacaatg
    27541 atagaaacca ttactcatgg tgcagagtgg tttatcgggc tgttccaaaa gggcggagag
    27601 gtgtttaccg ggatggtgac cggcattctt ccgctgttga ttagcctgct ggttatcatg
    27661 aacgcactga ttaattttat cggtcagcat cgtattgaac gttttgctca acgttgcgcc
    27721 ggtaaccctg tttcccgtta cctactgtta ccgtgcattg gcacgtttgt cttttgcaat
    27781 ccgatgaccc taagcctggg tcgctttatg ccggaaaagt acaaacccag ctactacgcg
    27841 gcggcctctt atagctgcca ctcaatgaat ggcctcttcc cccatatcaa ccctggcgaa
    27901 ctgtttgttt atcttggcat tgccagcggt ctgacaacgc tgaacctgcc acttggccca
    27961 ctggcggtga gttatctgct ggttggtctg gtcaccaatt tcttccgcgg ctgggtgacc
    28021 gatctgacca ccgccatttt tgagaaaaag atgggcattc aacttgaaca aaaagttcac
    28081 ctggcaggag caacatcatg acgcatattc ggatcgaaaa aggaacgggt ggctggggcg
    28141 gcccgcttga gctgaaagcc acgccgggaa aaaaaatcgt ctatatcacc gccggtaccc
    28201 ggcctgcgat tgttgacaaa ctggcacagc ttactggctg gcaggctatt gacggattta
    28261 aagaaggtga acccgcggag gcggaaattg gtgtcgcggt aatcgactgt ggcggcacat
    28321 tacgctgcgg catctatccg aaacgacgta ttcccaccat taatatccac tcgacgggca
    28381 agtccggtcc gctggcgcag tacattgtgg aagatattta tgtctctggc gtaaaagaag
    28441 aaaacatcac tgtagtgggt gatgcgacac cacaaccctc ttccgtgggc cgtgactatg
    28501 acaccagtaa gaaaatcacc gaacaaagcg atggtttact ggcgaaggtg ggaatgggca
    28561 tggggtccgc cgttgcggtg ctgtttcaat ctggtcgtga caccatcgac actgtattaa
    28621 aaaccattct gccgtttatg gcattcgtct cggcgctcat tggcatcatt atggcttctg
    28681 gccttggtga ctggattgcc cacggtcttg ctccgctggc gagccatcca ctgggtctgg
    28741 tcatgctggc gctcatctgc tccttcccac tgctttcacc tttcctcggc ccaggcgcag
    28801 ttatcgcaca ggttatcggc gtattgattg gcgtgcagat tggtctcggc aatattccgc
    28861 cgcatctggc tttaccggca ctgtttgcca tcaacgcgca ggcggcctgc gacttcatcc
    28921 cggtcggttt gtcgctggcg gaagctcgtc aggacacggt tcgcgtcggt gtcccttctg
    28981 tactggtgag ccgcttttta accggcgcgc cgactgtact gatcgcctgg tttgtctccg
    29041 gttttatcta tcaatagagg ctgaaacatg accgttattt atcagaccac catcacccgt
    29101 atcggcgcga gtgccactga cgccctcagc gaccagatgc tcatcacctt tcgtgaaggc
    29161 gcgccagcgg acctcgaaga gtattgcttc attcattgcc acggcgagtt gaaaggtgaa
    29221 ctccatcctg gtttgcaatt tacgctcggg cagcatcgct atccggtgac cgccgttggc
    29281 agcgtggcgg aagacaacct tcgcgaactg ggtcatgtca ccctgcgctt cgatggttta
    29341 aacgaagcgg aatttccggg cactgtccat gtggcaggcc ctgtacccga cgatatcgcg
    29401 ccgggatcgg ttttgaagtt tgaatctgtt aaggagtaaa aaatgaatca ggttgccgtt
    29461 gtcatcggtg gtgggcaaac cttaggcgcg ttcctgtgcc acggtctggc tgccgagggg
    29521 tatcgcgtcg cggttgtcga tattcagagc gacaaagccg caaatgtggc acaagaaatt
    29581 aacgccgaat atggtgaagg tatggcgtac ggttttggtg ctgacgccac cagcgagcaa
    29641 agcgttctgg cgctctctcg tggggtagat gaaatctttg gtcgcgtgga tttgctggtc
    29701 tatagcgccg gaatagcaaa agcagccttt atcagcgact tccagctcgg cgattttgac
    29761 cgttcgctac aagtgaatct ggtgggttat ttcctgtgtg cccgtgaatt ttcgcgtttg
    29821 atgatccgcg acgggattca gggtcgcatt attcagatca actcgaaatc cggcaaagtg
    29881 ggtagcaaac acaactctgg ctacagcgca gcgaaatttg gtggcgtcgg gctgactcaa
    29941 tcactggcgc tggatctggc ggagtacggc attacggtgc attcactgat gctcggtaac
    30001 ctgctgaaat cgccgatgtt ccagtcactg ttgccacaat acgcgaccaa gctgggtatc
    30061 aaaccggatc aagtcgagca gtattacatc gacaaagtac cgctcaaacg cggctgcgat
    30121 tatcaagatg tgctgaatat gctgctgttc tacgccagtc ctaaggcgtc gtactgcacc
    30181 ggacagtcga tcaatgtcac cggcggtcag gtgatgttct gatcaacagc ggagatccgt
    30241 taaggatctc cgtgagacta tagaatgcct gatgcgctac gctcatcagg catacaggac
    30301 ttccgccact acattaagga aaagttatgg tatccgcact catcaccgtc gccgttatcg
    30361 cctggtgtgc gcaactggcc ttaggcggct ggcaaatttc tcgttttaac cgtgccttcg
    30421 acacactatg ccagcaaggg cgggtcggcg tgggccgttc cagcgggcgc tttaaaccgc
    30481 gggtcgtggt cgccatcgcc ctggacgatc agcagcgcat cgtcgacacc ttgtttatga
    30541 aaggactgac cgtcttcgcc cgaccgcaaa aaattcccgc aattaccggt atgcatgcgg
    30601 gtgatttaca gcccgatgtg atctttcccc atgatccact atcacagaat gctctatcat
    30661 tggcgcttaa actgaaacgt ggataatttc gttgtgaatg ttacttgctt gcgaagttat
    30721 cattttgaaa cctaaatcag gtaatcacgc ccatgaaacc tcgtcagcgt caggccgcca
    30781 ttctggagta tctgcaaaag cagggtaaat gctcggttga agaattggcg caatactttg
    30841 acaccacagg cacaaccatt cgcaaagatc tggtcattct ggaacatgcc ggaaccgtca
    30901 ttcgtactta tggcggagtg gtgttgaata aagaggaatc cgatccgcct atcgatcata
    30961 aaacgctcat caacacccac aagaaagagc tgattgcaga agctgccgtt agttttattc
    31021 atgatggcga ctcaataatt cttgatgctg gcagtaccgt tttgcagatg gtccccctgc
    31081 tctcgcgctt taataacatc acggtgatga ccaacagtct gcatatcgtc aatgcgctat
    31141 ctgaactgga taacgaacaa accatcctga tgccaggcgg aacatttcgc aaaaaatcgg
    31201 cctcatttca cgggcaactg gcagagaatg ccttcgagca tttcaccttc gacaaattat
    31261 tcatgggtac tgacggcatc gatctcaatg cgggcgtaac caccttcaac gaggtctata
    31321 ccgtcagtaa ggcaatgtgc aatgccgcgc gcgaagtgat tttaatggcg gactcatcaa
    31381 agtttggccg taaaagcccc aacgtagttt gcagtcttga aagcgtcgat aagctgatta
    31441 ccgacgcagg tatcgatccg gcgtttcgtc aggcgctgga agagaaaggg atcgatgtga
    31501 tcataaccgg agagagcaat gagtgaagca ctactgaacg cgggacgtca gacgttaatg
    31561 ctggagttgc aggaagcaag ccgtttaccg gaacgtctgg gcgatgattt tgttcgcgcc
    31621 gccaatatca tcctgcactg cgaaggcaaa gtggtggttt cgggaattgg caaatcgggc
    31681 cacattggta agaaaatcgc cgcaacactt gccagtaccg gcacaccggc tttttttgtc
    31741 catccggcag aagcactgca cggcgatctg gggatgattg aaagccgcga tgtgatgctg
    31801 tttatctctt actccggtgg cgcgaaggaa ctggatctga ttattccgcg tctggaagat
    31861 aaatctatcg cgctgctggc gatgaccggc aaaccgacgt caccgctggg cctggcggca
    31921 aaagcggtgc tggatatctc cgtagaacgc gaagcctgcc cgatgcacct tgcgccgacc
    31981 tccagcaccg tcaataccct gatgatgggt gacgcgctgg cgatggcggt catgcaggcg
    32041 cgcggtttta atgaagaaga ttttgcccgc tcccacccag ctggggcact gggcgctcgc
    32101 ttgctgaata aagtgcatca tctgatgcgc cgtgatgacg ctatcccgca ggtagcgtta
    32161 gccgccagcg tgatggatgc gatgctggag ctcagccgca ccggtctggg gctggtggcg
    32221 gtatgtgacg ctcaacaaca ggtacaaggc gtctttaccg acggcgattt acgtcgctgg
    32281 ctggttggcg gcggcgcact caccacgcca gttaatgaag cgatgacgac aggcggcacc
    32341 acattacagg cgcaaagccg tgccatcgac gccaaagaga tcctgatgaa gcgcaaaatc
    32401 actgccgcac cggtggtaga tgaaaacggc aaactcaccg gcgcaattaa cttgcaggat
    32461 ttctatcagg ccgggattat ttaatccttc aatcccagac gtttcgccag ccgatgcagg
    32521 ttggcgacgt cagtttccag cattcgcgcg caggcagccc agttgtgatg attttgtgcc
    32581 agtgcctggc gaatggtttc acgctggaac gcttctgtcg cttcacgcag attttgctta
    32641 acaacgggca ccgctgccgc ttctggcggc ggcaacgtca cctcaggaaa agcaaaatgt
    32701 tgcgcctcaa gaatcacttc atcgccgctg cgagtggctc tcgacagtac taccgcccga
    32761 tgaatagcat gttccagttc gcgcacgttc cccggaaaac tgtagtgttg cagtaaattt
    32821 cgcgccccgg cacttaatac cacgcgggag agccccagcc gcaaacgaca ctgctcgcag
    32881 aaataccccg ccagcagaat gacatcatcg ccccgctcac gcagcggcgg caccgaaagt
    32941 ggaaacacgc tcaggcgatg aaacaagtca gcgcgaaatc gccctgccag tacctcttcg
    33001 cgtaagtcgc ggttagtcgc cgccagcacg cgcacatcga cccgcaaact gcggtcatcg
    33061 ccaacgcgct gaatatcgcc atactgcaac actcgcagca acttggcctg caaagccaac
    33121 gacaactcgc cgatctcatc gagaaacagt gtgccgttat ccgccatttc aaacttcccg
    33181 ctgcggttac tgatagcgcc agtaaacgcc cctttcacat gcccgaacag ttcactttcc
    33241 gccacacttt ccggcagtgc ggcacagttg agatagatca gcggattcac cgcccgtggc
    33301 gaggcttcat gaatcgcttt cgccaccagc tccttaccgg ttcccgtctc accgctgatt
    33361 aagacgttga gatcggacgc cgccacaatc tcaatctctt ttttcaattg cgtcatgcct
    33421 ggcgataagc caatcatctg cgtctgtttc accgcttcaa acggcgcggc atcgcccggc
    33481 agcatattct ggctttccag ttgctcaatc agcaacgcat tgcttaacgc tcccgccgcc
    33541 agcgcagcaa tcagccgtaa ctcttcatcg ctgaaaacat cgaactgatc gggctgcatc
    33601 ccgtcgagcg tcaatgcgcc aatcaggttt tgcccggcaa acaatggcag accaacgcag
    33661 gcgtgaacct tcagactctc ctgcccagga atcaaaccgt catagggatc gggcaattcg
    33721 ctgtctgcag gaaagcgcac cacgtccccg gcgcgggcaa tcgcttcaag ccgtggatgc
    33781 ccttccagcg caaagcgtct accgagtaca tcctttgcca gaccgtcgat ggcaagcgga
    33841 ataaactgcc gcgaatcgta acgtagcaac gcagacgcat cgcactccag cacctgacgt
    33901 agcgtggtga tcaggcgctg aaaacgatcc tgatgaccaa tcccacgctg caattcgatg
    33961 gcgatattcg ccagcacatc aacggaaaaa ctcatctttg cctcactgtc aatttgacta
    34021 tagatattgt catatcgacc atttgattga tagtcatttt gactactcat taatgagcat
    34081 acttttattt atatagtaaa aacaatcaga taaaaaactg gcacacaatc tgcaattagc
    34141 aagacatctt tttagaacac gctgaataaa ttgaggttgc tatgtctatt gtggtgaaaa
    34201 ataacattca ttgggttggt caacgtgact gggaagtgcg tgattttcac ggcacggaat
    34261 ataaaacgct gcgcggcagc agttacaaca gctacctcat ccgcgaagaa aaaaacgtgc
    34321 tgatcgacac cgtcgaccat aaattcagcc gcgaatttgt gcagaacctg cgtaatgaaa
    34381 tcgatctggc ggatatcgat tacatcgtga ttaaccatgc agaagaggac cacgctgggg
    34441 cgctgaccga actgatggcg caaattcccg atacgccgat ctactgtact gccaacgcta
    34501 tcgactcgat aaatggtcat caccatcatc cggagtggaa ttttaatgtg gtgaaaactg
    34561 gcgacacgct ggatatcggc aacggcaaac agctcatttt tgtcgaaaca ccaatgctgc
    34621 actggccgga cagcatgatg acttacctga caggcgacgc ggtgctgttc agtaacgatg
    34681 ctttcggtca gcactactgc gacgagcatc tgttcaacga tgaagtggat cagacggagc
    34741 ttttcgagca gtgccagcgt tactacgcca atatcctgac gccgttcagc cgcctggtaa
    34801 cgccgaaaat taccgagatc ctgggcttta acttaccagt cgatatgata gcgacctccc
    34861 acggcgtggt ctggcgcgat aacccgacgc aaattgtcga gctgtacctg aagtgggccg
    34921 ctgattatca ggaagacaga atcaccattt tctacgacac catgtcgaat aacacccgca
    34981 tgatggctga cgctatcgcc cagggaattg cggaaactga cccacgcgtg gcggtgaaaa
    35041 ttttcaacgt cgcccgaagc gataaaaacg aaatcctgac caatgtcttc cgctcaaaag
    35101 gcgtgctggt cggcacttcg acgatgaata acgtgatgat gccgaaaatc gccgggctgg
    35161 tggaggagat gactggttta cgcttccgta acaaacgcgc cagtgctttc ggctctcacg
    35221 gctggagcgg cggtgcggtg gatcgtcttt ccacgcgcct gcaggatgcg ggtttcgaaa
    35281 tgtcgcttag cctgaaagcc aaatggcgac cagaccagga cgctctggag ttatgtcgtg
    35341 aacacggtcg cgaaatcgcc cgtcagtggg cgctcgcgcc gctgccgcag agcacggtga
    35401 atacggtggt taaagaagaa acctctgcca ccacgacggc tgacctcggc ccacggatgc
    35461 agtgcagcgt ctgccagtgg atttacgatc cggcaaaagg cgagccaatg caggacgttg
    35521 cgccaggaac gccgtggagt gaagttccgg ataacttcct ctgcccggaa tgctccctcg
    35581 gcaaagacgt ctttgaagaa ctggcatcgg aggcaaaatg agtaacggca ttgtgatcat
    35641 tggttcgggc ttcgccgccc gccaactggt gaaaaatatt cgcaaacagg acgccactat
    35701 tccattaacc ctgattgccg ccgacagcat ggatgagtac aacaaacctg acctcagcca
    35761 tgttatcagt caggggcaac gtgccgacga ccttacccgc cagacggcgg gtgaatttgc
    35821 cgagcagttt aatctgcgcc tgtttccgca cacctgggtg acggatatcg atgccgaagc
    35881 ccgtgtggtg aaaagccaga ataatcagtg gcaatacgac aagctagtgc tggcaaccgg
    35941 tgccagcgcc tttgtcccgc cagtgcctgg gcgtgagtta atgctgacgt taaatagtca
    36001 gcaagagtat cgcgccagtg aaacgcaact gcgggatgcc cgacgcgtgt tgattgtcgg
    36061 tggtggcttg attggtagcg aactggcgat ggatttttgt cgtgcaggca aagcggtcac
    36121 gctaatcgac aacgctgcca gtattctggc gtcattaatg ccaccggaag taagcagccg
    36181 cttgcagcat cggttgacgg agatgggcgt tcatctgttg ttgaaatctc agttgcaggg
    36241 gctggaaaaa acagattctg gcattctggc aacgctggac cgccagcgca gtatcgaagt
    36301 ggatgcggta attgccgcca ccggactgcg cccggaaacc gccctggcac gacgcgccgg
    36361 gctgacgatt aatcgcggcg tttgcgtcga tagttatctg caaaccagta atgccgatat
    36421 ttacgcgctg ggcgattgcg cggaaattaa cggtcaggta ttgccgttcc tccagccgat
    36481 tcaacttagc gcgatggtgc tggcaaaaaa tcttctcggc aataacacgc cactgaaact
    36541 accggcaatg ctggtgaaaa tcaaaacgcc ggaattaccg ctgcatctgg caggcgaaac
    36601 ccagcgtcag gatttacgct ggcagattaa taccgaacgc cagggaatgg tggcgcgcgg
    36661 cgttgacgat gctgaccagc ttcgcgcctt tgtggtcagt gaggatcgga tgaaagaggc
    36721 atttggattg ttgaaaacgt tgccgatgta ggtgggctac tgtgcctaaa atgtcggatg
    36781 cgacgctggc gcgtcttatc caacctacgg ggagcgcatg tgttggtcac atactgcgtt
    36841 catgtcgcat ccacctactg tgcccaaaat gtcggatgcg acgctggcgc gtcttatccg
    36901 acctacgggg acgcatgtgt aggccggata aggcgtttac gccgcatccg gcaatggtgt
    36961 ccaaatgcaa cacgttttat ccgttctgga cttcacccgc taaccaacgc gccgcagcaa
    37021 taaccccctg ccccagagac aaaccgccat cacccgccgg taaactctgt ggaaagagca
    37081 atgtgaaatc agcgagataa tgcgccagac gtgcacgcag caaacggtta tgaataaccc
    37141 cgccgctaaa taccagcgta gtgataccac gcatcgtggc ctgctcacgc atcaacgcgg
    37201 caaaaccctg cgccagcgca tcatgaaacg cccacgcgcg ttgattaacc ggtgcctgcc
    37261 agttcagcca ctgctgccag aaagtggcga gatccagttg attgtccacc agcggcattg
    37321 tcaccggatg cgtcactccg tggcacgaag ctgcgagtgc ctccagagca caagccgctt
    37381 caccttcata acttaacgtg gctggcgcac agcccagtgc cgccgccacg gcatcgaaca
    37441 accgcccaca cgatgacgcc agcggcgcgt taattccacg ctcaatggcc cgcgccagca
    37501 cgctccagtt ttgctgttgc acacttgctg tttcagaata attctgccac tccggcacaa
    37561 agcgcaggca ctgcgccagc aggtttcgcc acggctgcgt cgccgccaaa tcgccacccg
    37621 gaagcgccac tgcaggcaag ccgcccaggt gctcacattc gcgatagttc acccgcagac
    37681 actcgccgcc ccacaaagcg ccgttctccc ccataccgat accatcgagc gtcaaagcaa
    37741 tgacatcacc gccatccagc ggccactgat gctctgccag acacgccgct gcgtgggcat
    37801 gatgatgcag caccgtttgc gtcggcatat tcatttcgct cgcccactgg ctggagacat
    37861 agcccggatg cacgtcatgc acaacgtatt gcggagtgaa atcgtagatg ttttgcatca
    37921 ggcgtaacgc ttcgcgccac tgcatctgga tgccatcgtc acttaaatcg cccagatgct
    37981 gactcaacac cgcttgttca ccgcgcacca ggcagaaggt gtttttcaga tccgcgccaa
    38041 gacacagcac aggcggaaca tttttaaagc ccggaggcaa aaccagcgca tccggcacat
    38101 acccgcgtga acggcgcagc atttcgccgc tttcgcgcac caccgaatca tccatccgct
    38161 gcacgatgtc gcggttatgt atcaagaatc cgtcggcaat gccctgcaaa tccgccagcg
    38221 cctgttcgtt gctgatagct ggtggtttac cgctcaggtt gccggaggtc atcaccagcg
    38281 ggcattgcag ttcctgtaac agcaaatgct ggagcgggtt cgcaggcaac attaccccga
    38341 cttcgttaag gtcaggggcg atatcatcac aaagctcagg aacgtatttt ttatccacca
    38401 gcacaatcgg cgcggcgggc gtggtaagca actggcgcgc agcgtctggt aaaccgtcag
    38461 ccactggcaa catgaccgcc agcggtttcg ccgggcgatg tttgcgcgcc cgcagtgtcg
    38521 ccaccgcgtt actgttacgt gcatcgcagg caagatgaaa tccgccaatc cctttgatgg
    38581 cgacaatgtt gcccattttt aactgtgcga tagccgcctg taatgccgcc tcttgttctg
    38641 catgttcacc atgacttacc cattcaagat gtgggccaca ctccgggcag gccaccggct
    38701 gggcgtggaa gcgacgatcg agcgggtcgc ggtactcttt gtcacaagcc ggacacaacg
    38761 gaaacgccgc catcacggta aacgggcggt cgtaaggcat ggcgcgaata atggtgaaac
    38821 gcgggccgca gtgggtacag ttgataaacg gataacgata acgccgttcg cctggggtat
    38881 tcatttcggc aagacaggca gggcaagtag cggcatcggg aacaatttgc gtattcatgg
    38941 tgccgcctgt gctctggcgt atagtgaact cggtgggcag ttgtgaccag atgtacggct
    39001 cacgctcgac gctatcaata cgcgccagcg gcgggcagtg ctgatgcaat tgaacaagaa
    39061 acgtttccgg gtcttccagc agccggactt ctacgccatc gccgtcatta cagacatcgc
    39121 cgtgaagatt taattgctgt gccagctgcc agacaaacgg acgaaaaccg acgccctgca
    39181 ctttgcctcg aatacgcagt tggacaccgc aagatgtgtt ttttgccatt gagttattcc
    39241 cgccatcatg aattgcgtaa cccgccctgc cggacacgac tgcgtcgtat ccggcagtct
    39301 caggtcggcg ataccgccag ctccgtattc tacgaatatt tccgggaatt cctttgatgc
    39361 cagaacagtt ctataagatt tttagaacat cagcgccgta cggcgacgtt tttctgcgct
    39421 cagttgttca agtttattac gatcgacaca aatcagcgca tgggtcgggc aagccgccat
    39481 acacgccggg ccgtcttcgc gatggttgca caggtcgcat ttattggctt cagctttgtc
    39541 agcccgtaca ttcagccccg cgccgctgtt gcggatcacc ggacgtacca ccacttccat
    39601 cgcgccatac gggcaagcca ccacgcaggt tttgcaacca atgcaacgtt cctgcatcac
    39661 atgaacaaac cctttatcac ggctgatagc accattcggg cagacgttag cgcacggtgc
    39721 atcttcacac tgacggcaga ctgtcgccgt ggaaatgttc acacctttaa tgacatggat
    39781 tcgcggtaaa aaggtttccg gggtcagcga tgcacagtcc tgattttcct gatgagaaac
    39841 cacgcacgcc acttcacagg tacggcaacc aatacattta ctcgcgtcag caatgatgaa
    39901 acggttcatc aaattctcca gcaatgacag ttaatgcgcc gatacattca caaatcatgc
    39961 cagtttttaa tttgctgtta tttaaggaaa ttaatttctg taatgcagga aaaaagatgt
    40021 catcgacact agtgacgatg acatgtgatg acaatgttta tcgcgaaagg gcaatgagtg
    40081 agtcgcggcg gatcagtttt ccgctgaagg ttttcggcgg tgagaaatcc ccgccatcga
    40141 gcataaaaat cagccgtcca ataatttcct gaatcatctc tgttaccgga atttttacgc
    40201 tggagagcgc cggaacggtg tagggggcaa ttgcgatatc atcgaatccg ataactgaca
    40261 cctgctctgg caccgctacg ccgcgctcgt gcaacgcttt catcgcacct atcgccatat
    40321 cgtcgttact ggcaactaac gcgctaaatt tagccccacg ttcgagcaac gtttctaccc
    40381 cttcggcccc gctggcgggc gtccatttac cgttagcgat aagtttttca ttgagcgcaa
    40441 taccatgctg cgccagcgcg tctttatacc cagcaagacg ttcaatgctg gtgggggaat
    40501 ccatcgagcc ggtaaggaaa gcaatctcct gatgtccggc gtttatcaac tctgccacgg
    40561 cattaaagct ggtctgttta tgatcgcacc agacgctatg gctgctgttt ttgcgcaggc
    40621 ggcgattaag caccattatc ggctgactgt gcgcgtcaat gatgtcatcg atctcatcca
    40681 cgcttaaaaa acgcgggtaa atcataatgg cgtcgcagcg caaatccagc agatactgaa
    40741 tcgcctggcg ctcttcttcg gcgctgtgtt taccatctgc caatagcaac tgccgccctt
    40801 tctcttccgc cattcgcgcg gcatgaaaga gtaattcact aaaataaatg ccgtggtaaa
    40861 gcgtgttggt cactaccagc cccagcgtct gagtactctt cgccgacaga ttgcgcgcca
    40921 gcaagtttgg gcggtaaccg ctctcttcta ccgcctgaaa cacgcgatct ttagtctcct
    40981 ggctgacgta gccattacct gaaagcacgc gggaaacggt cgcttttgaa accccggcgc
    41041 gcttcgccac ttccagcatc gtcgtcatca ttttcatccc tttacacgca atcaacgcag
    41101 tgtactgcac cgtttgccga ttgtccttgc gcaatcggcg ggaaaaatat tcaggtgacc
    41161 ggtttcacaa atataaaaaa tgaacaatta actctcttgc ttatttagtg acaactattc
    41221 atgattttgt gaaaccggtt tcctaattcc gtttcagcat cggcattttt ccgttacgtc
    41281 gactgataac aactacatct accctactga taacaggata aaatccgatg gccaaaaatt
    41341 atgcggcgct ggcacgctcg gtgatagcgg cactgggcgg cgttgataac atctcggcag
    41401 tcacgcactg tatgacgcgt ttgcgctttg ttatcaaaga tgaccagctt atcgacagcc
    41461 cgacgttaaa aaccatcccc ggcgtgctcg gcgtggtacg tagtgacaac cagtgtcagg
    41521 tgattatcgg caataccgtt tcacaagcct ttcaggaagt cgtcagcctg ctgccgggag
    41581 atatgcagcc cgcacagccc gtgggtaaac ccaaactcac gctgcgtcgc attggtgcgg
    41641 ggatcctcga tgcgctgatc agcaccatgt caccgctgat cccggcgatt atcggcggat
    41701 cgatggtcaa actgctggcg atgatcctcg agatgagcgg cgtgttgaca aaaggatcgc
    41761 cgaccttaac cattctgaat gtgattggtg acggtgcttt cttcttcctg ccgctaatgg
    41821 tcgcggcttc ggccgccatc aaatttaaaa ccaatatgtc gctggcgatt gccatcgcag
    41881 gtgtgctggt acatccgagc tttattgaac tgatggcgaa agcggcccag ggtgaacatg
    41941 tcgaatttgc cctgattccg gtcaccgcgg tgaaatatac ctacacggtg atcccggcgc
    42001 tggtgatgac ctggtgcctg tcatatatcg aacgctgggt ggatcgcatt acaccggctg
    42061 tgaccaaaaa cttcctcaag ccaatgctga ttgtgttgat tgccgcaccg ctggcaatcc
    42121 tgctgattgg cccgattggt atctggatcg gtagcgccat ttcggcgctg gtttacacca
    42181 ttcatggtta tctgggctgg ctttcagtcg ccattatggg cgcgctgtgg cctctgctgg
    42241 taatgaccgg gatgcaccgc gtctttacgc caaccatcat tcagaccatt gccgaaaccg
    42301 gcaaagaagg gatggtcatg ccgtcagaga tcggcgctaa cctgtcgctg ggcggttcat
    42361 cactggcggt ggcgtggaaa acgaaaaacc cggaactgcg ccagacggcg ctggctgcgg
    42421 cggcatcagc cattatggcg gggatttccg aaccggcgtt atacggcgtg gcgatccgcc
    42481 tgaaacgtcc gcttatcgcc agtcttatca gcggttttat ttgcggcgcg gttgccggta
    42541 tggcggggct tgccagccac tcaatggcag cgccggggct atttaccagc gtgcagttct
    42601 tcgatccggc gaatccaatg agcatcgtct gggtattcgc ggtcatggcg ctggcggtgg
    42661 tgctgtcgtt tatcctcaca ctgttgctcg gctttgagga tattcctgtt gaggaagcga
    42721 ctgccgaggc gcgaaagcat cagagcgtac aaccgaccgt cgccaaagaa gtaagtctta
    42781 attgaggatg aaaatgtcag tatttccaga aggtttttta tggggcggcg cgcttgccgc
    42841 caaccagtct gaaggtgcgt tccgtgaagg tggcaaaggg ctgaccactg tcgatatgat
    42901 cccacacggc gagcatcgaa tggcggtgaa actggggctg gaaaaacgtt ttcagttgcg
    42961 cgatgacgag ttttatccca gccatgaggc gacggatttt tatcatcgtt ataaagaaga
    43021 tatcgccctg atggcagaga tgggattcaa ggttttccgt acctcaattg cctggagccg
    43081 cctctttccg cagggcgata aaatcacgcc caatcagcag ggcattgctt tttaccgttc
    43141 tgtctttgaa gagtgtaaaa agtacggtat cgaaccgctg gtcacgttgt gccacttcga
    43201 tgtgccgatg catctggtca ccgaatatgg ctcctggcgt aaccgcaagc tggtggagtt
    43261 tttcagccgc tacgccagaa cctgctttga agcatttgat ggtctggtga aatactggct
    43321 aaccttcaat gaaatcaaca ttatgttgca tagcccgttc tccggcgcgg gtctggtgtt
    43381 tgaagaaggt gaaaatcagg atcaggtgaa atatcaggcc gcgcatcacc agctggttgc
    43441 cagtgcgcta gccaccaaaa tcgcccatga ggttaacccg caaaatcagg tgggctgtat
    43501 gctggcgggc ggtaacttct acccttacag ctgcaaaccg gaagatgtct gggcggcgct
    43561 ggagaaagat cgggaaaacc tgttttttat cgatgtgcag gcgcgcggcg cgtatccggc
    43621 ttactctgcc cgcgtatttc gcgaaaaagg ggtaaccatc aacaaagcac cgggcgatga
    43681 tgaaatcctg aaaaacaccg tcgattttgt ctctttcagc tattacgcct cgcgctgcgc
    43741 ctcggcggag atgaacgcca acaacagcag tgcggcgaac gtggtgaaat cgctgcgtaa
    43801 tccgtatcta caggtgagcg actggggctg gggaattgat ccactcggtc tgcgtatcac
    43861 catgaatatg atgtacgatc gttatcagaa gccgctgttt ctggtggaaa acggcctcgg
    43921 cgcaaaagat gaacttgctg ccaatggcga gattaacgat gactatcgca tcagctattt
    43981 acgcgaacat atccgcgcaa tgggcgaagc gattgcagat ggcattccgc tgatgggcta
    44041 caccacctgg ggctgtattg atttagtttc cgcctctacg ggtgaaatga gcaaacgcta
    44101 cggttttgtc tacgtagacc gtgacgacgc aggcaacggc acgctgacgc gcacgcgtaa
    44161 gaaatcgttc tggtggtata aaaaagtaat tgccagtaat ggggaagatt tagagtaagt
    44221 aaccgtgccg gatgcggcgt gaacgcctta tccggcctac atgtcgcacg aaccatgaag
    44281 gcccgataag cgcagcgcat cgggcaatct ggggtttggc atcagtcaaa atcaaagaaa
    44341 ccattccggc ttaacgccaa atcgggctga aagtgcctta atgtgggata tcgtcaggga
    44401 tcgttgtcca gacaaaatct gactgaccag ggatttagag ccgatttcgt tcttcaaatc
    44461 cgcataggtc agattatgtt gatcaatcaa cgtgcgaagt aacgccacgc caaccggcat
    44521 tgccgcaacg gctttgtcga actctgcaaa cttttcgttg ttgttttcat actctgcaat
    44581 gcggctcgcc agaaagtcga taagtggatt ggtgtcatct tcttcaatca aatattcgac
    44641 cagttccagt gcttcacgat aatcatcttc tgagtcactg cctcccagaa aggggactgc
    44701 attgaccagt tctcttgttg ctttaacagc gcgtgcagca ttagcagtca ttctttgttc
    44761 tctctgtagt aacgtgacgt ctgtctgcac gtaatccccc tcatagccac tctgcaaaaa
    44821 gtcagttctg gttcacccca taaagcacat ccccctttat caagcaacgc tgcgtctgcg
    44881 ccagatcttc cgtttgcggg tcgcgcccgg cgatgggttt gaggacgagt ttacggttgg
    44941 gggtataagt aaaacgcgtg agcgcgggat agcttaactt gccaaaccag ccgtgaacac
    45001 gctgccattt ggcgtctttt accgatacag actggttatc cagtaaccgg aatcaaactg
    45061 catatccagc aattgcgtgt ggccattcct ttgtaaatcc agcaaatcat caatgccagt
    45121 ttcacttgcg gtataaaaac catcggactg aaaaacacac gggcgacctt gctgattaaa
    45181 ggtcatcaga atcaaaaacg cgttaggtgc cagaccattt cctgaaatac cccgccagat
    45241 aaccagatcc tgaatgccgt tacgatcaag atcggcacgg taaagttgcg cattacccgc
    45301 catattttgc agcactccca caggcacaga ccaggattta ccggcgcgat ctttaccgtt
    45361 caattgccat tcactatcat catttggcat cttttcaagc gtcacgccag caacgccgac
    45421 ttttctaaaa tgccagtttt gctcggcatc ataaggcatg gtcaggagag gtttcagcgc
    45481 ccgcttttgc gcgtattcgc tgtcactcat tggcagatat tcggtaaagc tggtggcctg
    45541 tgcaggcagc actaagctca ataaaaaagc gcacatccct gttctgatgt tcaattccct
    45601 gaattccctg atggagaatt attcttcttc caccgctaac tgcgcaaatc caccatttcc
    45661 ttcccagcct tccagtcgtt gataaacggt ttctaccgca tctttaatcg gctgggtcat
    45721 cgggtagtaa aagccgacga tatccggctg aatgccgagg aaaatcactt caccaatgtc
    45781 ttcttttaac tggtcgataa ggtaattcaa cggcatgtta tgggtagtca tcataaacat
    45841 ctcggcgata tcatccgggt cgatgatgcg gatttcgccg gggtttagcc ccatatccgt
    45901 ggcgtcgaca atcagcagtc gtgtcgggcg cagttcacgg atggcgacga tgtcgttttc
    45961 cggtgcacta ccgccgtcaa tcaccaccca gttacctttc ggcgcggcgg cgcacttttc
    46021 cgccagcagc gggccagcgc catcatcgcc catcatgcta ttgccaacac agagtaaaac
    46081 gtcagtcacg cagtctcctc accatcaaat agatggcgtt ttcctgatga atatcatgca
    46141 gcatacttaa cagcattgta ctccattcct tctgctgcga agtttgcgct gcccgggctt
    46201 tatcgaatgc gttggccagc atcggcacat ggttaatgtc gatgacgatc tcgccatatt
    46261 tcggtacgcc ttccattttg cggcgggctt cgctgcccgc ctccagagtg gcaatccatg
    46321 ccagatattc atcccatggg caggtgagcg ccgcttccag gcaatcgata accccaaggt
    46381 ggtgaccaat cgccaggctg taatagacca cctgctgcgc ctcggcgggc gtggcatcgt
    46441 tctcatcaat aaatttacgg ctcagttgac tgaacaccac cttttcactc atcggatacg
    46501 cgcctcttca acaacatgat tcagatggct gacaatctcg ttcagacgcg gatcgttttc
    46561 cgcttccagc cagcgtgcaa cctgttcttc gccctgccct aactgtgtca ggaagtcatc
    46621 ggcaatctga cgaccgtaac gataacctgc caggcggcgt gcttcgcgat ccactttcac
    46681 acgcagcggc tgcaccatat cgccatgcag gatttccgcc ggttgttcat cctgctcacc
    46741 cgggccacgg gcgtgaattt tctgctccag caggccgagc gccattgcaa agccgtacag
    46801 cgtggcggca ggcgttggcg ggcaaccagg gatgtagaca tctaccggga cgattttatc
    46861 cgtaccgccc cacacgcagt agagatcgtg gaagatcccg ccactgttac cgcaggcacc
    46921 gtaggagata cagattttcg ggtccggcgc ggactgccac gcacgcagcg caggggatcg
    46981 cattgcacgg gtgaccgcgc cggtaaacag taaaatatcc gcatgacgcg gtgaaggaac
    47041 gactttaatg ccgaagcgtt ctgcatcaaa cagcggcgaa agcgtggcga aaatttcgat
    47101 ttcgcagccg ttgcagccgc cgcagtccac gcggtaaacg taggcagaac gtttgatttt
    47161 tttcagtaac gacgccttca tgctggcgat ggattcatcc accgtcatcg ggaccggaat
    47221 gccgttggcg tcacgggggc ctaataaatt gctcatcaga tggcctcttt catatggcga
    47281 gtcagttcaa tacggtcgga cggcaccagg catttctggc gcttacattc cgggcaagtc
    47341 tcaaagcttt cgcggtggtt ttccgcgcgg ctgtcgccgt tgtgcttaag tagcgcaatg
    47401 gcgtagtcga tctctttctg gacagcgaaa ggacgattgc agacgcgaca gttgcacagc
    47461 gcgaagcggg actgttgcag gaagtcttct ttcttccaca ccgccagttc gtactcttgc
    47521 gacagtttga tcgccgccgt cgggcaaact tcttcgcagc gtccacagaa gatgcagcgt
    47581 ccaagattga actcccacgc aagctctcct gtggcgaggt cggtttcaac cgttaaggcg
    47641 tttgacgggc aggcattgac gcaggccgcg cagccgatgc actgctgcgg gttctgctct
    47701 ggcttaccac ggaagttttt atcaaccgca atcggctcca gcggataaga cgaggtcgcc
    47761 gtgccggttt tgataacttt tttgataaag gtaaacatgg cgattcctta tttcagcggc
    47821 gagtttttac gctcaatgct gtagcgctcg agttctttgt acggcaccac tttgctcttc
    47881 ttcttacgta catcgaccac cgtcatgcgg tcggtacagg agtagcaagg gtccaggcta
    47941 ccgataatca gcggcgcatc ggaaacggta ttgccacgca gcatgtagcg cagggtcggc
    48001 cagttcgcgt aggtcgcggc acggcagcgc cagcggtaca gcttctggtt gtcgccggtc
    48061 atgctccagt ggatatcgtc gccgcgcggc gcttcggcaa agcccagcgc gaaacggtgc
    48121 ggaatgtagg taaagccttc caccatcagc ggaccgcccg gcaggttatc cagaccgtag
    48181 tcgatcatgt tcagcgcggt ataaacttcg ttgatacgca ctttcagacg ggaaataacg
    48241 tcgcagccct gctcgctgtg gacttccatt ggcagcaggc cataaccgac aaacgggtga
    48301 tcggcgcgag tatcgcgggc gtgaccgctg gcgcgaacca tcgggccaac gttactgaaa
    48361 tcgcgggcga tttccgggtc cagacgacca atgccgacgg tgcgctgttc catgttcggc
    48421 gtactcagca gcacatccac cagctcctgc acttcacgac gcatctgttg cgccagctgg
    48481 cgggtctgga tcatgtcatc tttcaacaga tcgcgacgaa tcccgccgat caggttcagg
    48541 ccgtaggttt tacgcgcacc ggtgaggatc tctgccattt tcatggaggt ttcacgcacg
    48601 cggaagaact gcataaagcc ggagtcaaag ccggtaaagt ggcaggcgag accgaggttg
    48661 agcagatgcg agtgcaggcg ttccacctcc agcagaatgg cgcggatcat ctgcgcacgt
    48721 tctggcacct gaatacccat cgcgttttct accgacgtgg tgtaggcagt gctgtgggca
    48781 aagccgcaga tcccgcacac acggtcagag aggaaggtca cttcgttgta acccatacgg
    48841 gtttccgcca gtttttccat gccgcgatga acgtagaaca gacggtagtc ggcgtcgata
    48901 atgttttcgc catcgacgaa cagacggaag tggcccggtt cgtcagaagt gacgtgcagc
    48961 ggaccaatcg gcacgacgtt gtttttcttg tcgcccagtt cgttgatgaa ctcgtaggtt
    49021 tcagcatcgg tagtcggtgc cggacgctga cgataatcca tgctgtcttt acgcagcgga
    49081 taaagttcat ccggccagtc atccggcagc accagacgac gttcatccgg cagaccaacc
    49141 ggaatcaaac cgtacatatc gcgcacttcg cgctcgcccc acaccgccgc cggaacgcgc
    49201 ggcgtcacgg acggatactc cggtttgttg gcgtcaactt cgacgcgaac cgttacccaa
    49261 cacttggtgc ccttctccat cgacagcacg taataaacgg cgtaatgacc attcagtttg
    49321 cgttcgtcgt taccaaacag caccgacagc cagccaccct gcttgtagta gagaaactcc
    49381 accacttccg gcaggtagtt caccttcacg gtcacggtca gctgatcttt ggtctgccag
    49441 gcgtggtcca gcacgacgcc cggaaatgcc tcattcagcg cggcgagata atgttgacct
    49501 aatttttctt cagacatgct caaactctct ttaatcacgc cgccagcaag gagacgaacg
    49561 ctaaaaatgc aaagccaaac ccagcccagg taatgcgcgg agtaatatca agacgcagac
    49621 gggccatgct gttttcgaac agcgcgataa ccaggacgcc gacgaccagt ttgacgatgg
    49681 caatcaccag cgccagcagc agcccaccgg cggtgaaggt ttccatctgt ccccacggga
    49741 taaatacgcc gacgaacatc tgcagcacca ccagctgttt caggctgata ccccatttca
    49801 tgacgccaaa gccgctgccg ctgtattcag agagcgggcc ttcctgcagc tcctgctcgg
    49861 cttccgccag gtcgaacggc agtttgccca tttcgataaa ggtggcgaac gcacaggcac
    49921 aaagcgccag taccagcggg atgctctggc tcagcggcca gtgataaacg gtgtcggtga
    49981 tgttgctgat gttggtggaa ccggcaacct gtgcggcaac ccacagacca agcagcagca
    50041 tcggttcgac cagcacgcca agcatcgctt cacggctcgc gccgatagcg gtaaacgggc
    50101 taccggtatc cagaccagaa atggcaaaga agaaacgcgc gatggcaaag agatacagta
    50161 aggtgattaa atcacccagt tgcggcagcg gagaaccgac ggtcaccacc ggcagcgcag
    50221 tagcgatagt cagcatgacg cccaccatca catacggcgt caggcggaac acccagccgg
    50281 aggcatccgg gccaacgctc tggcggccca gcagtttgat aatgtcacga tactcctgca
    50341 acacacccgg cccacgacgg ttatgtaagc gggctcgcgc cacgcgggta ataccggaga
    50401 gcagcggcgc aacggcaaat agcaccagcg cctgaattaa cggatataaa acactcattc
    50461 tcaggctcct cgtgaaacaa taatcaccac cagcaccgcc agttcaacca gcgccatccg
    50521 gcggaacagc aacgcactcc cctcgcactg ccagcccggc accagagaca ccggattcag
    50581 ccatttgcgt agtttcagca ccggcgcaaa cgcctgtttc accggcatgg caaaaccgtg
    50641 agcggtaatc accattgatt tttcgtggtc gtagccgcac acccaggccg caccgcggga
    50701 acgcgacggc aaacgatcgc ctttgcaaat cgccataatg atgaatggca gcagcgggca
    50761 ggcaatcagc agcaacgtga tcatcggttg agaaacggtg gtgttagcag gctccagcgg
    50821 cagaggtaca gcagcagaga gcatcggcag cagccacggc gcagcaacac cgccaattac
    50881 gcagcaaatc gccagtgcca ctacgcttac gctcatcagg agcggcgcac aggtggcgtt
    50941 ttcggcttct ttggtacgcg gcgcgccgag gaacgtgacg ccatagactt tcgccataca
    51001 catcaccgcc agcgcaccgg taattgccag acccacagcg agcagcggcc ccagcagacg
    51061 ggcaacaaac gcgccactat tgctcagttt gaaaaaggat tgatagataa cccattcccc
    51121 ggcaaacccg ttcagcggcg gcagcgcagc cattgccatc aacccgacta acatggcgat
    51181 ggagataacc ggcattttct tgccaatacc accgagtttt tcgatatcgc gatgaccggt
    51241 acggaaccag acgctccccg ccccgaggaa cagtacgctt ttgaacaggc tatggttaag
    51301 cagatggtac agaccaccga ccagaccaag ggcaatcagc gccggttgtt cgagcgcgat
    51361 acccgttacg ccagcgccca gccccagcag gataatgccg atattttcca gggtgtggta
    51421 agccagcagg cgctggatgt tgtgttccat caacgcatac agaccaccga cgaacgcggt
    51481 gatcatgccg agcaccagca gcgcgatccc ccaccacagc ggcgcgttac cacccagcag
    51541 cgacagggtt aaaatgccca gcaggccaat tttcatgact accgtagaaa acaacgcggc
    51601 agctggcgca gaggcgttag catgtgcctg cggcacccag ccgtgcagcg gaataatccc
    51661 ggccagcagg ccaaagccaa tcacaccgag cagccagata tcggaaccga gcggcagctg
    51721 ttgcatacgc atatccagca ggcgcagatc cagcgtgcca taacgctgcc acagcagcca
    51781 gcaagcaatc gccagcagca gagtgccaag acgccccagc gcaaaccaca gtttgccctc
    51841 tttgctgttg ctggtgagga acaccgcaca caaggccatg acttcggcca ttaccacgaa
    51901 catgccgagg ttgctggcaa tgacggcgca gacggcggca gccatcaaca tattgatctg
    51961 caagccgttg cacttcacct gcgcgtggcg atgccagtca atgttataga ggctgacaaa
    52021 cagaccgcaa aggccgaggg taatcagcca aatcgcgtta agcggggaga tttgcacatc
    52081 gtagcttacc agcgacagcg caccgctcac gccaaccgcg ccagtcagca cagtgaagcc
    52141 cgcggctgcc gtatacaggc taccaaccgc gccgccaatt ccggctatcc agccacttag
    52201 cgtcttttga aaagaaaaga gaaatgccag aacagcggcg gcaataaacc acgccacgcc
    52261 gctattgatc agggaaattg cgctcattta gcctctccac tttgagcctg ctgaaacaag
    52321 gtgagatcgc caaagtccgt attaaaagtc agctcacgct tacgtttgct gacgcgggcg
    52381 atatcggtgt tatccaccag atgcagggct ttggtcgggc acatccgcac gcaggccgga
    52441 ccttgttcat caaaactgca aaggtcacat ttgacggcga tcgcgcgaat acccggcacc
    52501 cagtcaagca gcgtactgat acgcgccgga gcaggcggtg ccggtggcgc tttcggggta
    52561 ttggcatttg caggaatatc cagcggacgg ctgccggaaa attcaattgc gccaaacggg
    52621 caggcgatgc cgcacagctt gcagcttacg cacaggcttt cattcaactg cacggcccca
    52681 tcaacgcggg tgatggcgtt aaccgggcag accaccgcgc agggtgcatc ttcacagtgg
    52741 tgacagagct gcggcgcaga ttctttttca tttagcatca ctctcaggcg cggcattgat
    52801 tgcaggccgt gctggcgatg cgtctctgaa caggcggcct cacaagtgtg gcagccgata
    52861 cagagcgtgg agtcagcaat tacaaaacga ttcaccaggc attcctcagg tgattgtcat
    52921 ttttgacgaa aacatgccga tgaaatgtca ttttcgacac tcatcgacac gcccatcccc
    52981 aaacaggcgt aacgcctgca aaacgggcaa agcctcagct catgctgccg ggctttgtcc
    53041 ctttaccagt tggcttaaat tcaccggcac attgttttca atggcggtgt ataagctgtc
    53101 gtaaacgcca gcgatttttt cgagatagtg ttccagaacc tgttcgcgat cgcggatgtt
    53161 gaccttgcca tcaatcatgc cgtcaatgct cagttgcgcc tgcgcaatcc gctgtttgtc
    53221 ttcgccatct tttgtttcga cgtaatactc gatggtgtgg ctgttaaagc catccgccag
    53281 ggtgacgtaa atacgaaaat gttcaaaaag gacgaaacag agttctttgt ccggcgcgca
    53341 gctcatggcg tgatgcaggc gcgctttcga taacgtgatc ccctgaacca gcgaattgca
    53401 gtagatgtgc cactggtcct gtaggcgacg atgccgctgt gcgatgtaat cggctttctc
    53461 gcttatttcc caaatagtca ttgtcaggtt acccgtttaa cagagatgcc agctttaagc
    53521 attttttgtg ccaactttta atttattgtt attaaagaga tttttaagct aaagatgaat
    53581 ttcgtcgccg tgtcgacgtg tcatttcgac atcatcgaca ttattcaccg cagggataat
    53641 caacactggc acaattattg cttgtagctg gcaatagtta atgggaggcg atatgcacga
    53701 aataaccctc tgccaacggg cactggaatt gatcgaacag caggccgcaa aacacggcgc
    53761 aaaacgcgta actggggtct ggctcaaaat tggcgcattt tcttgtgtcg aaaccagctc
    53821 tcttgccttt tgttttgatc tggtttgccg cggcagcgtg gcggaaggtt gtaaactgca
    53881 cctcgaagaa caagaggccg aatgctggtg tgaaacatgc caacagtatg tgacgctact
    53941 gacccagcgc gtccgccgct gtccacagtg tcatggtgac atgctacaga ttgtggcaga
    54001 cgacggttta cagattcggc ggatagaaat agaccaggag tgagcgatgt gtacaacatg
    54061 cggttgcggt gaaggcaacc tgtatatcga gggtgatgaa cataaccctc attccgcgtt
    54121 tcgtagcgcg ccatttgccc cggcggcacg cccgaagatg aaaatcaccg gcattaaagc
    54181 gcctgaattt acccccagcc agactgaaga aggcgacctg cattacggtc atggcgaagc
    54241 gggcactcac gcaccgggca tgagccagcg tcggatgctg gaagtcgaaa ttgacgtgct
    54301 ggacaaaaac aaccgtctgg ctgaacgcaa ccgcgcgcgc tttgctgccc gcaagcaact
    54361 ggtgctcaac ctggtttcca gccctggttc cggtaaaacc accctgctga cggaaacctt
    54421 aatgcgcctg aaagacagcg ttccgtgcgc ggttattgaa ggtgaccagc aaaccgtgaa
    54481 cgatgccgca cgcattcgcg ctaccggcac accagcgatt caggtgaaca ccggtaaagg
    54541 ctgccatctt gacgcacaga tgattgccga cgccgcaccg cgtctgccac tggacgataa
    54601 cggtattctg tttatcgaaa acgttggcaa cctcgtatgc ccggccagct tcgatctcgg
    54661 tgaaaaacac aaagtggcgg tgctttccgt taccgaaggt gaagacaaac cgctgaaata
    54721 tccgcatatg tttgccgccg cctcgctgat gctgctcaac aaagttgacc tgttgccgta
    54781 tctcaacttt gacgttgaaa agtgcatcgc ctgcgcccgc gaagtcaatc cagaaattga
    54841 aatcatcctt atttccgcca ccagcggcga agggatggac cagtggctga actggctgga
    54901 gacacagcga tgtgcatagg cgttcccggc cagatccgca ccattgacgg caaccaggcg
    54961 aaagtcgacg tctgcggcat tcagcgcgat gtcgatttaa cgttagtcgg cagctgcgat
    55021 gaaaacggtc agccgcgcgt gggccagtgg gtactggtac acgttggctt tgccatgagc
    55081 gtaattaatg aagccgaagc acgcgacact ctcgacgcct tacaaaacat gtttgacgtt
    55141 gagccggatg tcggcgcgct gttgtatggc gaggaaaaat aatgcgtttt gttgatgaat
    55201 atcgcgcgcc ggaacaggtg atgcagttaa ttgagcatct gcgcgaacgt gcttcacatc
    55261 tctcttacac cgccgaacgc cctctgcgga ttatggaagt gtgtggcggt catacccacg
    55321 ctatctttaa attcggcctc gaccagttac tgccggaaaa cgttgagttt atccacggtc
    55381 cggggtgccc ggtgtgcgta ctgccgatgg gtagaatcga cacctgcgtg gagattgcca
    55441 gccatccgga agtcatcttc tgtacctttg gcgacgccat gcgcgtacca gggaaacagg
    55501 gttcgctgtt gcaggcaaaa gcacgcggtg ccgatgtgcg catcgtttac tcgccgatgg
    55561 atgcgttgaa actggcgcag gagaatccaa cccgcaaagt ggtgttcttc ggcttaggtt
    55621 ttgaaaccac catgccgacc accgccatca ctctgcaaca ggcgaaagcg cgtgatgtgc
    55681 agaattttta cttcttctgc cagcatatta cgcttatccc gacgcttcgc agtttgctgg
    55741 aacagccgga taacggtatc gacgcgttcc tcgcgccggg ccacgttagt atggttatcg
    55801 gcactgatgc ctataatttt atcgccagcg attttcatcg tccgctggtg gtggctggtt
    55861 tcgaaccgct tgatctactg caaggcgtgg tcatgctggt ggagcagaaa atagcggccc
    55921 acagcaaggt agagaatcag tatcgtcggg tggtaccgga tgccggtaac ctgctggcgc
    55981 aacaggcgat tgccgatgtg ttctgtgtca acggcgacag cgaatggcgc ggcttaggcg
    56041 tgattgaatc ttctggtgta cacctgacgc cagattatca acgattcgat gccgaagcgc
    56101 atttccgccc ggcaccgcag caggtctgcg atgacccgcg cgcacgttgt ggtgaagtat
    56161 taacgggcaa atgtaagccg catcaatgcc cgctgtttgg taacacctgt aatcctcaaa
    56221 ccgcatttgg tgcactgatg gtctcctccg aaggagcgtg cgccgcgtgg tatcagtatc
    56281 gtcagcagga gagtgaagcg tgaataatat ccaactcgcc cacggcagcg gcggccaggc
    56341 gatgcagcaa ttaatcaaca gcctgtttat ggaagccttt gccaacccgt ggctggcaga
    56401 acaggaagat caggcacgtc ttgagctggc gcagctggta gcggaaggcg accgtctggc
    56461 gttctccacc gacagttacg tcattgaccc gctgttcttt cctggcggta atatcggcaa
    56521 gctggcgatt tgcggcactg cgaatgacgt tgcggtcagt ggcgctattc cgcgctatct
    56581 ctcctgtggc tttatcctcg aagaaggatt gccgatggag acactgaaag ccgtagtgac
    56641 cagcatggca gaaaccgccc gcacggcagg cattgccatc gttactggcg atactaaagt
    56701 ggtgcagcgc ggcgcggcag ataaactgtt tatcaacacc gctggcatgg gcgcaattcc
    56761 ggcgaatatt cactggggcg cacagacgct aaccgcaggc gatgtattac tggttagcgg
    56821 cacgctcggc gaccacgggg cgactatcct taacctgcgt gagcagctgg ggctggatgg
    56881 cgaactggtc agcgactgcg cggtactgac gccgcttatt cagacgctgc gtgacattcc
    56941 cggcgtgaaa gcgctgcgtg atgccacccg tggtggtgtg aacgcggtag ttcatgagtt
    57001 cgcggcagcc tgcggttgtg gtattgaaat ttcagaagcg gcactgccgg ttaaacctgc
    57061 cgtgcgcggc gtttgcgaac tgctgggact ggacgccctt aactttgcca acgaaggcaa
    57121 actggtgatc gccgttgaac gcaacgcggc agagcaagtg ctggcagcgt tacattccca
    57181 tccactggga aaagacgcgg cgctgattgg tgaagtggtg gaacgtaaag gtgttcgtct
    57241 tgccggtctg tatggcgtga aacgaaccct cgatttacca cacgccgaac cgcttccgcg
    57301 tatatgctaa taaaattcta aatctcctat agttagtcaa tgaccttttg caccgctttg
    57361 cggtgctttc ctggaagaac aaaatgtcat atacaccgat gagtgatctc ggacagcaag
    57421 ggttgttcga catcactcgg acactattgc agcagcccga tctggcctcg ctgtgtgagg
    57481 ctctttcgca actggtaaag cgttctgcgc tcgccgacaa cgcggctatt gtgttgtggc
    57541 aagcgcagac tcaacgtgcg tcttattacg catcgcgtga aaaagacacc cccattaaat
    57601 atgaagacga aactgttctg gcacacggtc cggtacgccg cattttgtcg cgccctgata
    57661 cgctgcattg cagttacgaa gaattttgtg aaacctggcc gcagctggtc gcaggtgggc
    57721 tataccccaa atttggtcac tattgcctga tgccactggc ggcggaaggg catatttttg
    57781 gtggctgtga atttattcgt tatgacgatc gtccctggag cgaaaaagag ttcaatcgtc
    57841 tgcaaacatt tacgcagatc gtttctgtcg tcaccgaaca aatccagagc cgcgtcgtca
    57901 acaatgtcga ctatgagttg ttatgccggg agcgcgataa cttccgcatc ctggttgcca
    57961 ttaccaacgc ggtgctttcc cgcctggata tggacgaact ggtcagcgaa gtcgccaaag
    58021 aaatccatta ctatttcgac attgacgata tcagcattgt cttacgcagc caccgtaaaa
    58081 acaaactcaa catctactcc actcactatc ttgataaaca acatcccgcc cacgaacaga
    58141 gcgaagtcga tgaagccgga accctcaccg aacgcgtgtt caaaagtaaa gagatgctgc
    58201 tgatcaatct ccacgagcgg gacgatttag ccccctatga acgcatgttg ttcgacacct
    58261 ggggcaacca gattcaaacc ttgtgcctgt taccgctgat gtctggcgac accatgctgg
    58321 gcgtgctgaa actggcgcaa tgcgaagaga aagtgtttac cactaccaat ctgaatttgc
    58381 tgcgccagat tgccgaacgt gtggcaatcg ctgtcgataa cgccctcgcc tatcaggaaa
    58441 tccatcgtct gaaagaacgg ctggttgatg aaaacctcgc cctgaccgag cagctcaaca
    58501 atgttgatag tgaatttggc gagattattg gccgcagcga agccatgtac agcgtgctta
    58561 aacaagttga aatggtggcg caaagtgaca gtaccgtgct gatcctcggt gaaactggca
    58621 cgggtaaaga gctgattgcc cgcgcgatcc ataatctcag tgggcgtaat aatcgccgca
    58681 tggtcaaaat gaactgcgcg gcgatgcctg ccggattgct ggaaagcgat ctgtttggtc
    58741 atgagcgtgg ggcttttacc ggtgccagcg cccagcgcat tggtcgtttt gaactggcgg
    58801 ataaaagctc tctgttcctc gacgaagtgg gcgatatgcc actggagtta cagccgaagt
    58861 tgctgcgtgt attgcaggaa caggagtttg aacgtctcgg cagcaacaaa atcattcaga
    58921 cggacgtacg tttaattgcc gcgactaacc gcgatctgaa aaaaatggtc gccgaccgtg
    58981 agttccgtag cgatctctat taccgcctga acgtattccc gattcacctg ccgccactac
    59041 gcgagcgtcc ggaagatatt ccgctgctgg cgaaagcctt taccttcaaa attgcccgtc
    59101 gtctggggcg caatatcgac agcattcctg ccgagacgct gcgcaccttg agcaacatgg
    59161 agtggccggg taacgtacgc gaactggaaa acgtcattga gcgcgcagta ttgctaacac
    59221 gcggcaacgt gctgcagctg tcattgccag atattgcttt accggagcct gaaacgccgc
    59281 ctgccgcaac ggttgtcgcc caggagggcg aagatgaata tcagttgatt gtgcgcgtgc
    59341 tgaaagaaac caacggcgtg gttgccgggc ctaaaggcgc tgcgcaacgt ctggggctga
    59401 aacgcacgac cctgctgtca cggatgaagc ggcttggaat tgataaatcg gcattgattt
    59461 aactgcaaat tgccggacag atctgcctgt ccggcatact attcacgagt ttttttcgga
    59521 cgatattttt ccggcagttc tggcaccgga cgcttgtcat cgatgagatg acgcacggtt
    59581 aagatcggat gacgccacag cattcgcggc ccggcccaac gcataatctg tttcatctct
    59641 tcacgctttg ctggctggta acagtgcacc ggacactgct tacaggctgg tttctcttcg
    59701 ccgaacacac atttatccag ccgcttttgc gcgtaaacaa acaacgcctc gtaatgctcc
    59761 ggctccgctg acgcctgcgg gcatttcgct tgataaagat cgatcatttt tttaatcgtc
    59821 agtttttcac gagagatacg cttgccggac atgctgcctc cacctcatta agatgtattt
    59881 atattacatc ttaatcttaa agggcactat gactctaaag aagaaaggtt cgccaaccga
    59941 cgcaattttg cgtacttgct tcataagcat cacgcaaaag ctgcaaaaca gcatctttcc
    60001 cggaaccagc atcaagaact caccgttcgc ttcttcccct gaaatgatta actccggtat
    60061 catgtgcgcc ttatgtgatt acaacgaaaa taaaaaccat cacaccccat ttaatatcag
    60121 ggaaccggac ataaccccat gagtgcaata gaaaatttcg acgcccatac gcccatgatg
    60181 cagcagtatc tcaggctgaa agcccagcat cccgagatcc tgctgtttta ccggatgggt
    60241 gatttttatg aactgtttta tgacgacgca aaacgcgcgt cgcaactgct ggatatttca
    60301 ctgaccaaac gcggtgcttc ggcgggagag ccgatcccga tggcggggat tccctaccat
    60361 gcggtggaaa actatctcgc caaactggtg aatcagggag agtccgttgc catctgcgaa
    60421 caaattggcg atccggcgac cagcaaaggt ccggttgagc gcaaagttgt gcgtatcgtt
    60481 acgccaggca ccatcagcga tgaagccctg ttacaggagc gtcaggacaa cctgctggcg
    60541 gctatctggc aggacagcaa aggtttcggc tacgcgacgc tggatatcag ctccgggcgt
    60601 tttcgcctga gcgaaccggc cgaccgcgaa acgatggcgg cagagctgca acgcactaat
    60661 ccggcggaac tgctgtatgc agaagatttc gccgagatgt cgctgattga aggccgtcgc
    60721 ggcctgcgcc gtcgcccgct gtgggagttt gaaatcgaca ccgcgcgcca gcagttgaat
    60781 ctgcaatttg gcactcgcga tctggtcggt tttggcgtcg agaacgcgcc gcgcggactt
    60841 tgtgctgccg gttgtctgtt gcagtatgcg aaagataccc aacgtacgac tctgccgcat
    60901 attcgttcca tcaccatgga acgtgagcag gacagcatca ttatggatgc cgcgacgcgt
    60961 cgtaatctgg aaatcaccca gaacctggcg ggtggtgcgg aaaatacgct ggcttctgtg
    61021 ctcgactgca ccgtcacgcc gatgggcagc cgtatgctga aacgctggct gcatatgcca
    61081 gtgcgcgata cccgcgtgtt gcttgagcgc cagcaaacta tcggcgcatt gcaggatttc
    61141 accgccgagt tgcagccggt actgcgtcag gtcggcgacc tggaacgtat tctggcacgt
    61201 ctggctttac gaactgctcg cccacgcgat ctggcccgta tgcgccacgc tttccagcaa
    61261 ctgccggagc tgcgtgcaca gttagaaact gtcgatagtg caccggtaca ggcgctacgt
    61321 gagaagatgg gcgagtttgc cgagctgcgc gatctgctgg agcgagcaat catcgacaca
    61381 ccgccagtgc tggtacgcga cggtggtgtt atcgcatcgg gctataacga agagctggat
    61441 gagtggcgcg cgctggctga cggcgcgacc gattatctgg agcgtctgga agtccgcgag
    61501 cgtgaacgta ccggcctgga cacgctaaaa gttggcttta atgcggtgca cggctactac
    61561 attcaaatca gccgtgggca aagccatctg gcacccatca actacatgcg tcgccagacg
    61621 ctgaaaaacg ccgagcgcta catcattcca gagctaaaag agtacgaaga taaagttctc
    61681 acctcaaaag gcaaagcact ggctctggaa aaacagcttt atgaagaact gttcgacctg
    61741 ctgttgccgc atctggaagc gttgcaacag agcgcgagcg cgctggcgga actcgacgtg
    61801 ctggtgaacc tggcggaacg ggcctatacc ctgaactaca cctgcccgac ctttattgat
    61861 aaaccgggca ttcgcattac cgaaggccgc catccggtgg ttgaacaggt gctgaatgag
    61921 ccgtttatcg ccaacccgct gaatctgtcg ccgcagcgtc gcatgttgat catcaccggt
    61981 ccgaacatgg gcggtaaaag tacctatatg cgccagaccg cgttgattgc gctaatggcc
    62041 tatatcggca gctacgtacc ggcgcaaaaa gtcgagattg gcccgattga ccgtatcttt
    62101 acccgcgtag gcgcggcaga tgacctggct tccgggcgtt cgacctttat ggtggagatg
    62161 accgaaaccg ccaatatttt acataatgcc accgaatata gtctggtatt gatggatgag
    62221 atcgggcgtg gaacgtccac ttacgatggt ctgtcgctgg cgtgggcgtg cgcggaaaat
    62281 ctggcgaata agattaaggc attgacgctg tttgccaccc actatttcga gctgacacag
    62341 ttaccggaga aaatggaagg cgtcgccaac gtgcatctcg atgcactgga gcacggcgac
    62401 accattgcct ttatgcacag cgtgcaggat ggcgcggcga gcaaaagcta cggcctggcg
    62461 gttgcagctc tggccggcgt gccaaaagag gttattaagc gcgcacggca aaagctgcgt
    62521 gagctggaaa gcatttcgcc gaacgccgcc gctacgcaag tagatggtac gcaaatgtct
    62581 ttgctgtccg taccagaaga aacttcgcct gcggtcgagg cactggaaaa cctggacccg
    62641 gattcactga ctccgcgtca ggcgctggag tggatttatc gcttgaagag tctggtgtaa
    62701 taaaaattcc cgatagtctt ttgctatcgg gaatattaac gacaactgac gaatcaaata
    62761 aaaataccct gtataatagg aaagcttatt ttacagggta aaaccatgcc atctacacgc
    62821 tatcaaaaaa tcaatgccca tcactatcgc catatatggg tcgttggtga tattcatggt
    62881 gaatatcagt tattacaatc ccgcttacat caactctctt ttttccccga aaccgactta
    62941 cttatttctg tcggcgataa tattgatcgt gggccggaga gtcttgacgt cctgcgcctg
    63001 ctaaaccagc cctggtttat ctccgttaaa ggcaaccacg aagcaatggc gctggatgcg
    63061 ttcgcgaccg gcgatggcaa tatgtggctt gccagcggtg gtgactggtt tttcgattta
    63121 aatgattcag agcaaaaaga agctacagat ctgttgctga aattccatca ccttccacat
    63181 attattgaaa tcactaacga caacataaaa tatgtcatcg cacatgcaga ttatccgggg
    63241 agtgaatatc tctttggtaa agaaatagcg gagagcgaat tactctggcc tgttgatcgt
    63301 gtgcagaaat cgcttaatgg cgagttacaa caaataaacg gcgctgatta ttttatattt
    63361 ggacatatga tgtttgataa cattcagacg ttcgctaacc agatttatat tgataccgga
    63421 tcgccgaaaa gtgggcggct gtcattttat aaaataaggt agtctcatgc ttcttctgtg
    63481 aagcatgagt aaccagatgt tattgcaggc cattattcat ttttcgctac cagcaaagag
    63541 agatcctgct tcgccagcgc gcgactggca ctctccggca aaccgtcgtc tgtaataatc
    63601 tgatcaaact cgcttaatgg taacgccagc catgtcgcca cctgaccata tttcgtcgca
    63661 tcacagacca aaactcgctg gcggctggca ctggcaatcg cccgtttcac cgtgacttta
    63721 tcttccgctg gcgtagaaat cccccgcaca ctccatgacg atgcagaaat aaaagcctga
    63781 tcaatcatca ggctgcgcag catggtcgca gcggcttccc cgacacagga acggttttcc
    63841 cgacacactg caccgccagt gtgaataatt gtgcaattac tgttgtcgag caagtagttc
    63901 gcaataacga aatcgtttgt gaccacagtc agtgactcca tgtgaatcag atgctgtgct
    63961 atcgctaacg tggtcgttcc cgcatccaga tagatacaac ttcccggctg aacaagactt
    64021 gccgccagct tgccaatagc cgctttttgc gtcattgcca gcgcagtttt tacctgatga
    64081 gaaggttcat gcgccacgcg tcccggagac tggacgcctc cggacaccag cacaacggct
    64141 ccctgctgct ccagtttttg taaatcccga cgaatggtca tatgtgacac attcattctg
    64201 tccgttagtt cagcaatact gacaatgcct ttttcagcta ccatctcaag gatgatttgg
    64261 cgacgctcta cgggtatcaa cttttgctcc ttcctttgtc ctgctgacat tctacgctat
    64321 ttgcctgcga aacgtgcgcg gcgcaactaa cgcttagttc acataaaata acacacaatg
    64381 ttaatttatg tgaatcagct caccatatcg ttatcttcca gcgcttatat tcacaatatc
    64441 aaacaaaata tcacttaaat taacaaggag agcagatgaa aacgggatct gagtttcatg
    64501 tcggtatcgt tggcttaggg tcaatgggaa tgggagcagc actgtcatgt gtccgcgcag
    64561 gtctttctac ctggggcgca gacctgaaca gcaatgcctg cgctacgttg aaagaggcag
    64621 gtgcttgcgg ggtttctgat aacgccgcga cgtttgccga aaaactggac gctctgctgg
    64681 tgctggtggt caatgcggcc caggttaaac aggtgctgtt tggtgaaaca ggcgttgcac
    64741 aacatctgaa acccggtacg gcagtaatgg tttcttccac tatcgctagt gctgatgcgc
    64801 aagaaattgc taccgctctg gctggattcg atctggaaat gctggatgcg ccagtttctg
    64861 gtggtgcagt aaaagccgct aacggtgaaa tgactgtcat ggcctccggt agcgatattg
    64921 cctttgaacg actggcaccc gtgctggaag ccgttgccgg aaaagtttat cgcataggtg
    64981 cagaaccggg actaggttcg accgtaaaaa ttattcacca gttgttagcg ggcgtacata
    65041 ttgctgccgg agccgaagcg atggcacttg cagcccgtgc ggggatcccg ctggatgtga
    65101 tgtatgacgt cgtgaccaat gccgccggaa attcctggat gttcgaaaac cggatgcgtc
    65161 atgtggtgga tggcgattac accccgcatt cagccgtcga tatttttgtt aaggatcttg
    65221 gtctggttgc cgatacagcc aaagccctgc acttcccgct gccattggcc tcaacagcat
    65281 tgaatatgtt caccagcgcc agtaacgcag gttacgggaa agaagacgat agcgcagtta
    65341 tcaagatttt ctctggcatc actctaccgg gagcgaaatc atgatcaaga ttggcgttat
    65401 cgccgatgat tttaccggcg cgacggatat cgccagtttt ctggtggaaa acggtctacc
    65461 aacggtacaa attaacggtg ttccaacagg taaaatgccg gaagcaatcg acgcactggt
    65521 gatcagcctg aaaacgcgct cctgtccggt ggttgaagcc acacagcaat cgctggcggc
    65581 tctgagctgg ttgcaacagc aaggttgcaa acagatctat ttcaaatact gctctacttt
    65641 cgacagtacg gcgaaaggta atatcggccc ggttaccgat gcattaatgg atgctctcga
    65701 cacgccgttt acggtcttct ctccggccct gccggtcaac ggacgtacgg tttatcaggg
    65761 gtatttgttc gtaatgaatc aactgctggc cgaatccggg atgcgccatc acccggtaaa
    65821 tcccatgacc gacagctatc ttccccgtct ggttgaagcg caatccacag ggcgctgcgg
    65881 cgtcgtttcg gcacatgttt tcgaacaagg tgtggatgcc gttcgtcaag agctggctcg
    65941 cttacagcaa gagggctacc gctacgcggt gcttgatgcg ctgaccgaac accatctgga
    66001 aattcaggga gaagccttgc gcgatgcccc actggtaacg ggcggttctg gtctggcgat
    66061 tggcctggcc cggcagtgga cgcaagaaaa cggtaaccag gctcgcaaag cagggcatcc
    66121 gctcgctggg cgcggcgtag tgctctccgg ttcatgctct caaatgacca accgccaggt
    66181 agcacattac cgtcaaattg caccagcccg tgaagttgat gtggcacgct gcctctcaac
    66241 tgaaactctg gctacttatg cacacgaact ggcagagtgg gttctgggcc aggaaagtgt
    66301 acttgctcca ctggtttttg ccaccgccag cactgacgca ttggcagcaa ttcaacagca
    66361 atacggtgca caaaaagcca gtcaggcagt agaaacactg ttttctcaac tagcggcgcg
    66421 gttagcggcg gaaggcgtgg cacgctttat tgtcgcaggc ggtgagacct ccggcgtagt
    66481 cacacagagc ctgggcataa aagggtttca tattggccca accatttccc ccggcgtgcc
    66541 gtgggtaaac gcactggata agcctgtctc actcgccctt aaatctggca acttcggtga
    66601 tgaagccttt ttttcacgag cccaaagaga gtttttatca tgagcgattt cgcaaaagta
    66661 gagcagtctt tgcgagagga gatgacgcgg attgccagtt cattctttca gcgcggctat
    66721 gcaaccggtt cggctggcaa tctgtcgctg cttttacctg acgggaattt actggcgaca
    66781 ccgacaggtt catgcctggg caatctcgat ccgcagcggc tttccaaagt cgccgcggat
    66841 ggcgaatggt taagtggtga caaaccctcg aaagaggtgc tctttcatct ggcgctgtat
    66901 cgcaacaatc cgcgctgtaa agcggtggtg catttgcaca gcacatggtc gacggcgctt
    66961 tcctgcctgc aagggctgga cagcagcaac gttattcgtc cgttcacacc atacgtggtg
    67021 atgcggatgg gaaatgtccc gctggtgcct tattaccgac cgggcgataa acgcatcgca
    67081 caggatctgg cggaactggc agcagacaat caggcttttt tactggcaaa tcatggccca
    67141 gtggtttgcg gtgaaagcct gcaagaagcc gccaacaata tggaagagct ggaggaaacg
    67201 gcaaagctga tttttattct cggtgaccgc ccgatccgtt atctgaccgc aggtgaaatt
    67261 gcggaattaa ggagttaatg caatgcctcg ttttgcagct aatttatcca tgatgttcac
    67321 cgaagtgcct tttattgaac gcttcgccgc agcgcgaaaa gccgggttcg atgctgtgga
    67381 atttctgttt ccctatgact actccaccct gcaaatccaa aagcaactgg agcaaaacca
    67441 tctgacactg gcgctgttca ataccgctcc cggagatatt aatgcggggg aatggggatt
    67501 atccgccctt cccggacgtg agcacgaagc acacgcagac atcgacctgg cactggaata
    67561 tgcattggcg ctcaattgtg aacaagtcca tgtgatggca ggcgtcgtgc ccgctggcga
    67621 agacgctgaa cggtaccggg cagtatttat cgataatctc cgctacgctg ccgaccgctt
    67681 tgcgccacac ggcaagcgaa tattagttga agcactcagc cccggcgtta agcctcatta
    67741 tctcttctcc agccagtatc aggcactggc tattgttgaa gaggttgcgc gagataacgt
    67801 gtttattcaa ctagatactt ttcatgcgca aaaagtagat ggcaacctga cgcatttaat
    67861 ccgcgactac gctgggaaat atgcgcacgt acaaattgcc ggactacctg atcgacatga
    67921 accggacgat ggagaaatca actatccgtg gctgttccgc ctgttcgatg aggtgggata
    67981 tcagggctgg atcggttgtg aatataaacc tcgtggcctc accgaagaag ggcttggctg
    68041 gtttgacgcc tggcgctaat tcgttaaatc cctaattaca acgtacccat acatcccccc
    68101 atctacggat ggggtaggtt aactataact ttcagacagg gtttcccatg tccacaatta
    68161 cattgttatg cattgcgtta gctggcgtaa tcatgctgtt gctgctggtc atcaaggcaa
    68221 aggtacaacc attcgttgct ctgctcctcg tcagcctgtt agtcgcactt gcggcgggta
    68281 taccggcggg cgaagtgggt aaagtgatga tcgccgggat gggcggtgtg cttggttccg
    68341 tcactattat tattggtctg ggcgctatgc tggggcgtat gatcgaacac tctggtggtg
    68401 cagagtcact ggctaattat ttcagtcgca agttaggtga caaacgaact atcgctgcgc
    68461 tgactctggc agcgttcttc ctcggtattc ccgtcttctt tgatgtcggc tttattattc
    68521 ttgcgccaat catttacggt tttgccaagg ttgccaaaat atcgccactc aaatttggcc
    68581 tgcctgtcgc tggaatcatg ctaactgttc atgtggcggt accgccgcat ccaggccctg
    68641 tcgccgcagc ggggttactc cacgcagaca tcggctggct aaccatcatc ggtattgcga
    68701 tttctattcc cgtagggatt gttggctact ttgcagcgaa aataatcaat acgcgccaat
    68761 atgcgatgtc ggtggaagta ctggaacaga tgcaactggc tccggccagt gaggaaggcg
    68821 caacaaaatt aagcgataaa ataaatccac cgggcgtcgc gctggtcacc tcgctaattg
    68881 ttattcctat cgcgattatc atggcgggta cggtttccgc aacactgatg ccgccttcgc
    68941 atcccctgct tggtacgcta cagctgatcg gctcaccaat ggtagctcta atgattgcgc
    69001 tggtgctggc attctggtta ttggctttac gtcgcggctg gagcttacaa cacaccagcg
    69061 acattatggg ctcagccctt cccactgcgg cggtagtgat tttggttact ggtgctggag
    69121 gggtatttgg caaagtgctg gtggaatcgg gcgtcggcaa agcccttgcc aatatgctac
    69181 aaatgattga cctgcctctg ttaccagccg catttattat ttcactggct ctgcgtgcat
    69241 cgcaggggtc agcaaccgta gcaatcctga caaccggcgg gttactctca gaagcggtga
    69301 tgggattgaa tccgattcag tgcgtattgg tgacgctggc agcctgcttt ggtgggcttg
    69361 gcgcgtcaca tattaatgac tcagggttct ggattgtgac caaatatctg gggttgtcgg
    69421 tagcagacgg tctgaaaacc tggacagtgt taacgaccat tctcggtttt accggattct
    69481 taattacctg gtgcgtatgg gcggtaattt gaccttaata aaaaggtccg atgggcatcg
    69541 gaccttttat ttaacgctta ccttccacca gcagcggcgg gatgctcggc accattggcg
    69601 cgtcatcaat atctttctgc gtcatgcgga acgcttcggg ataatgttcg cggctggtac
    69661 ggcgcagcgg ttcggtatcg cgcagcggtt cggtatcgcg ccaggtataa aggcaatgct
    69721 ggcactgata taccgtccag acatctttca ccggcgattt cgccatcact tcaatctgtt
    69781 catcggcaca acgtggacaa atcatcttct tctccttatt tacgtgcagc cagcatagcg
    69841 gtcagttttt cagcccaggc tttggtttcc ggtaaatcca ccaccggttg gctgtagtga
    69901 ccacggttgt ccggggcgac aggcgtagtg gcgtcgataa tcagcttgtc ggtgatcccc
    69961 gcagggcttg agcctggatc gagttccagc acggacatat tcggcaactg caccaaatcc
    70021 cctgccgggt tcactttcga ggagagcgcc cacatcacct gcggcaggtt gaacgggtca
    70081 acgtcttcat cgaccataat caccatcttc acgtagccca gaccatgcgg cgtggtcatt
    70141 gcgcgcaggc ccaccgcgcg ggcaaagccg ccgtagcgtt ttttggtgga gataatcgcc
    70201 agcaggccat gggtgtacat ggcgtttacc gcctgcactt cagggaactc ggctttcagc
    70261 tgctgataca gcggcacgca ggtggctggc cccatcaggt agtcgatttc ggtccacggc
    70321 ataccgaggt acagcgattc gaaaatcggc ctggtgcggt aagagacttt atcgatgcgc
    70381 accacggtca tgttacgccc gccggagtag tgcccggtaa actcaccgaa cggcccttcg
    70441 atttcgcgtt tacggctttc gatgacccct tcgaggatca cttctgaacc ccacggcaca
    70501 tcaaaaccgg tcaacggggc ggtggcgatc gggtacgggc tttcacgcag cgcgcctgcc
    70561 atttcgtact cggactgatc atatttcagc ggcgtggccc ccatcagcgt gatgatcgga
    70621 tcgttaccga gcgtgatcgc aatcggcaga tcttcaccgc gctcttctgc tttatgcaga
    70681 tgcagggcga tatcgtgcat cggcaccggt tgcaggccga gcttacgctt gcccttcact
    70741 tccatgcggt agatgccgac gttctgcttg ccgaagttat ccgggtcgag cggatcgcgg
    70801 gaaaccacgc acgctttgtc gagatagaaa ccgccatcgc catcgtttaa acgaaacagc
    70861 ggcaggatat cgaacaggtt aatctcttcg ccatcgacgg tgttctgcgc ccaggctgga
    70921 ttggcgcggc gctccggggc aatcgggaag ttatcccagc ggcggataaa ctcatcaatc
    70981 tgctttttga ccggggtgtt tggtggcagg ccaagggaaa tcgcgtggtt ctgccaggaa
    71041 ccgatggtat tcatcgtcac gcgggcatcg gtaaagccgc gaatattatc aaaccacagc
    71101 gcgggcgcgc cgtcgccgat acgcccggtg gcgttagctg ctgctgccag atccggctca
    71161 gcgttcacct cttcgctgat tttcagtaac tggccgtggt catcaagcgc ctgtaaaaag
    71221 ctgcgtaaat catcaaatgc cattattcat tctcctgaga aaaattccgg gcctgcggca
    71281 atccttgcca gcgcctggcg tggggatgtt cgaggccaaa ttgatccagc acgcgggcta
    71341 ccacatggtg gacaatgtca tctaccgttt cgggatggtt ataaaaggca ggcatcggcg
    71401 gcaccatcgc cacgcccatg cgtgaaagtg cgagcatatt ttcgagatgg atggtgctaa
    71461 gcggcatttc tcgcggcacc agcaccagtt tgcggccttc tttgagcacg acgtccgccg
    71521 cgcgccctac caggccatcg gcgtaaccag cgcggatacc ggcgagcgtt ttcatactgc
    71581 acggaataac gatcatgccg tcggtacgaa aagaaccgga tgagatgatc gccgcctgat
    71641 ccgccgggtt atggctgaag tctgcgaggg cagcaacatc gcgagcgctg taaggcgttt
    71701 ccagttcaat ggtggttttc gcccacttcg acatcaccag atgagtctcg acattcggca
    71761 tctcccgcag cgcttgcagt aatgccacac caagaggcgc accggtagcc cctgtcatcc
    71821 cgacgatcag tttcattgct tatcctctta attgttcgta tacgaacgtt attattaaca
    71881 tactccttca tttccacagc ggcaaggatt gaaaagtaac aacatcttct tcgtcactaa
    71941 aaaaccgcta tgatagcaac agagaattag cccggagttt acatggcgtt acgaaataaa
    72001 gcgttccatc agttacgaca gctttttcag cagcacacgg ctcgctggca gcacgagtta
    72061 cctgacctga ccaaaccaca gtacgcggtg atgcgcgcca ttgctgataa acctggtatc
    72121 gaacaggtgg cactgataga agcagctgtc agcaccaaag caacgttagc agaaatgttg
    72181 gcaagaatgg aaaatcgtgg cttagtcaga cgtgaacatg accccgctga taagcggcga
    72241 cgctttgtct ggttgactgc cgaaggcgaa aagatacttg ctgcggcgat acccattggt
    72301 gacagcgtgg atgaggaatt tttggggcgt ttgagtggag aagagcagga gctgtttatg
    72361 cagctggtgc gcaagatgat gaacgcatag ggggcaaatt tccggatgcg acgctatcgc
    72421 gtcttatcca gactacaaat acaccgactc accaaccgaa agaaaaaagg ccagcctcgc
    72481 ttgagactgg cctttctgac agatgcttac ttactcgcgg aacagcgctt cgatattcag
    72541 cccctgcgtt tgcaggattt cgcgcaaacg gcgcaggcct tcaacctgaa tctggcgaac
    72601 acgttcacgg gtgaggccaa tttcacgacc tacatcttcc agtgttgccg cttcgtaccc
    72661 cagcaaaccg aatcgacgtg ccagcacttc acgctgtttg gcgttcagct cgaacagcca
    72721 tttgacgatg ctctgcttca tatcgtcatc ttgcgtggta tcttccggac cgttctcttt
    72781 ttcatcggcc aggatgtcca gcaacgcttt ttcggaatca ccacccagcg gggtgtctac
    72841 cgaggtaatg cgctcgttaa gacgaagcat acggctgacg tcatcaactg gcttatccag
    72901 ttgctctgcg atctcttccg cacttggttc atggtccagc ttatgggaca actcacgtgc
    72961 ggttcgcagg taaacgttca gctcctttac gatgtgaatc ggcaaacgaa tagtacgggt
    73021 ttggttcata atcgcccgtt caatcgtctg gcgaatccac caggttgcgt atgttgagaa
    73081 gcggaaacca cgttccgggt caaacttctc taccgcgcgg atcagcccca ggttgccctc
    73141 ttcgataagg tccagcaacg ccagaccacg attgccataa cggcgggcaa tttttaccac
    73201 cagacgcaag ttactctcga tcatccggcg gcgagaggcg acatctccac gcagtgcgcg
    73261 acgcgcaaaa taaacttctt cttcggccgt taacagtggt gaataaccaa tctcaccaag
    73321 gtaaagctga gtcgcgtcca acacacgctg tgtggctccc tgcgataaca gttcctcttc
    73381 ggccaaatcg ttatcactgg gttcctcttc tactaaggcc ttttcgtcaa aaacctcaac
    73441 tccgttctca tcaaattccg catcttcatt taaatcatga actttcagcg tattctgact
    73501 cataaggtgg ctcctacccg tgatcccttg acggaacatt caagcaaaag catggttccg
    73561 ccgatttatc gctgcggcaa ataacgcagc gggtttacgg atttcccctt gtaacgaatt
    73621 tcaaaatgca agcgtgttga actggttccg gtgctaccca tggttgctat tttttgcccc
    73681 gccttcactt cttgttgttc ccggaccagc attgtgtcgt tatgggcgta ggcactcagg
    73741 taatcatcat tatgtttgat gataatcaga ttaccgtagc cgcgcagcgc gttaccagca
    73801 taaacaacgc ggccatctgc ggtcgcgata attgcctgtc ctttgctgcc tgcgatatca
    73861 atccccttgt tgcccccctc agaagcgcca aaggtttcga tcactttgcc ctcagtcggc
    73921 cagcgccagg tggagatagg cgtactggtt gatgtactgc tgacagttgg ctcggttgtg
    73981 cttgctgttg gtaccgttac aggcgctgtg accgtggtcg cagctggctt gttgttcggc
    74041 aacattttgt tagcactctg ttcacccgaa gattcagaat acgtaattgt cggttgcgac
    74101 gcaacagcaa cggtggaatt ttgtgcaggc ttgatcacaa ctccttgctc tgctgcgtcg
    74161 gcctgggtaa tggcatttcc gccagtgatt ggcgtaccgg aagcattacc cacctgcaag
    74221 gtctgaccaa cgttcagcgc gtatggtgcc tgaatattgt tgcgctgagc aaggtcacgg
    74281 aaatcgttgc cagtaatcca ggcgatatag aaaagtgtgt cgcctttttt cacggtatag
    74341 gtactgccgc tataactgcc tttcggaatg ttcccatact gacggttata gacgatgcgt
    74401 ccgttttcca tctgtaccgg ctgctgagct actggctgca ctggctggat ttgcggttgt
    74461 tgagtagcct gaatttgtgg ctgctgtacc ggctgaattt gcggttgctg ggctgtagac
    74521 gtcgtcccca ttttcggcgg cggcgtaatc aacataccag aattggtatt tgcaggcgca
    74581 ttgccattaa cggagctgac cggtgccggt ggatttgaag tgtcagaaca gcctgccagc
    74641 catagcgaaa ccagtgacaa agccgcaatg cggcgaacgg tgaattttgg gcttcccgcg
    74701 ctcatttatc ccccaggaaa aattggttaa taaccagtga cataattacc gtgcaaggca
    74761 ccctactgaa cactggaaaa gatgttcacg atacgctgac ctgcggcaaa ataaccagga
    74821 aaaatccagg tatttcctca cgttttaagc cagctcaccc ttcactaaag ggacaaagcg
    74881 cacggcctcc acggtatcga taataaattc gcctccccga cgacgcaccc gtttcaaata
    74941 ctggtgctcc tcccctacgg gtaagacgag aatcccgcct tcgtccagct gcgtcattag
    75001 cgcagttgga atttccggcg gtgccgccgt aacaatgata gcgtcaaacg gcgcgcgtgc
    75061 ctgccaacct tgccatccat cgccatgacg ggttgaaaca ttatgtaaat caagattttt
    75121 caggcggcga cgcgcctgcc actgcaaacc tttaatccgt tcgaccgagc aaacatgctg
    75181 gacaagatgc gccaggattg ccgtttgata tcccgaacca gtgccaattt ccagcacccg
    75241 cgactgcggc gtcagctcga gtaattcggt cattcgcgcc accatatacg gctgcgaaat
    75301 tgtctgcccc tgacctatcg gcaaggcgat attgtcccag gctttttgtt caaacgcttc
    75361 atcaacgaat ttttcacgcg gcacggcggc aagtgcattc agcacctgct catcctgaat
    75421 accttgcgca cgtaattgat ccagaagtgc ttgtacgcgt ctgcttacca ttgcgtgcca
    75481 actcccacgc tgtttaacca gtctgaaacc acatcttgcg cgctatgcgc agttaaatcc
    75541 acatgcagcg gcgtgatgga gacatagccc tcatctaccg cagcaaaatc ggtccccgga
    75601 ccggcatcac atttaccgcc cggcgggcca atccagtaca gcgtattgcc gcgcggatct
    75661 tgctgcggga tcacctgatc tgccggatgt cgtgtaccgc agcgcgtcac gcgaatacct
    75721 ttgatttgat ccaagggtaa atccggaacg ttaatattaa gaatacgccc ggtgcgcaac
    75781 ggctctttac acagtgcgcg caaaattgaa caggttaccg ccgcggcagt gtcgtaatgt
    75841 ttatgcccgt caagcgagac ggcaagcgcc ggaaaaccta aatgacggcc ttccatcgcg
    75901 gcggctaccg taccggaata aataacgtca tcccccagat tcggcccggc gttaattccg
    75961 gacacaacaa tgtccgggcg cggacgcatc agagcattca cgccaagata gacgcaatcg
    76021 gtcggggttc ccatttgcac agcaatatca ccattttcaa aggtaaacgt gcgcagggag
    76081 gattccagtg tcagagaatt tgaagcgccg ctgcggttac gatcgggggc gaccacctga
    76141 acgtcagcaa actcacgcaa ggctttcgcc agcgtttgta taccgggtgc atgtaccccg
    76201 tcatcattac tcagcaatat gcgcataatc acctgttgtg ttgataagtt ccctgacaac
    76261 gctggttgca aaactacccg ccggaagcca gaaacggatc tctacggtga cgtcatccca
    76321 ccaattccag cttaattgtt gcggatacag caacatcgct ctgcgcgcgg cttcaacttt
    76381 ttcgcgcacc agtaaagctt gtaattcagt ttctgcggcg acagctgcct tttcgaatgc
    76441 cagcgcttca cgctgagttc cccattcgcc actgcccggc aatgcggcgg ttatcatcag
    76501 ctctttatcg ttgacgcgac gctgtaattc cgccagttct tcggtggttg ctacaaacca
    76561 gctaccacgt ccggctaatt gtagcgcatc gccgtcaaca acttgattaa cgtctgcttt
    76621 tttgaggcgc tcagcaacaa tctgattaaa caacgcactg cgggctgccg acaaccaaaa
    76681 actccgttta ttgcgatcgc gcaccggagt attggtttgc gcccagcgca gcgcgccctg
    76741 caagttgcta ccgccaatcc caaaacgttg ggcaccgaag tagttcggta caccttttac
    76801 gcaaatatcg atcagacgtt gttcaacgtc atcgcgattg ctcacttcgc gcagaaccag
    76861 ggtaaaggcg ttacctttca gcgcgcctaa acgcagcttg cgcttgtgcc gcgcatactc
    76921 cagcacctgg cagccttcca gttgaaaggc gctcagatcg ggcatttcct tgcctggcac
    76981 gcgagcgcat aaccactgtt ccgtaacagc atgtttgtct ttttgcccag cgaagctgac
    77041 ttcacgggca tgaattttca ggaatttcgc cagtgcatcc gccacaaaac gggtattgca
    77101 gccgtttttg aggattctaa ccagaatatg ctcaccttca ccatcaggct caaagcccaa
    77161 atcttccacc accacaaagt cttccggatt ggctttcagc agcccggtgc cttgcggttt
    77221 accgtggagg taagtgagat tatcaaactc aatcattttg ttgccttaat gagtagcgcc
    77281 accgcttcac aggcaatccc ttccccacgt ccggtaaatc caagtttttc cgtagtagtg
    77341 gctttcacgt taacatcatc catatggcag ccgagatctt cggcaataaa cacgcgcatt
    77401 tgtggaatgt gcggcaacat cttcggtgcc tgagcgatga tagtgacatc gacgttgccc
    77461 agggtataac ccttcgcctg aatacgacgc caggcttcgc gtagcagctc gcggctgtcg
    77521 gcacctttaa atgccggatc ggtatccggg aacagcttgc cgatatcccc cagcgccgcc
    77581 gcgccaagca atgcatcggt caacgcatgg agcgccacgt cgccatcaga atgcgccagc
    77641 aatccttttt cgtaaggaat gcgtacgcca ccaatgataa ttgggccttc accgccaaag
    77701 gcatgtacgt caaaaccgtg tccaattcgc attatgtatt ctcctgatgg atggttcggg
    77761 tgaggtaaaa ctcggccagt gccaaatctt ccgggcgcgt gactttaatg ttatccgcac
    77821 ggccttcgac caactgagga tggaatccgc aatattccag cgccgaggct tcgtcggtaa
    77881 tagtcgcgcc ttcatttaga gcgcgcgtca gacagtcatg taacagctca cgagggaaaa
    77941 attgcggcgt cagcgcgtgc cataagccgt tgcgatcaac ggtatgagca atggcatttt
    78001 tgcccggttc ggcacgtttc atcgtatcgc gcactggtgc ggctaggatc cctcccgtgc
    78061 ggctggtttc gctcaacgcc aacaatcgcg cgaggtcatc ctgatgcaga caaggacgag
    78121 cggcgtcatg caccaatacc cactgcgcgt cgccagcggc tttcaaacct gccagcacgg
    78181 aatcggcacg ctcatcaccg ccatctacaa cggtgatttg cggatgattc gccagaggaa
    78241 gttgtgcaaa acggctatcg ccaggactta tggcaatgac gacacgtttc acccggggat
    78301 gcgccagcaa cgcatgcacc gagtgttcaa gaatggtttg attaccgatt gagagatatt
    78361 gcttaggaca ttccgtttgc attcgacggc caaatccggc cgccggaacc acggcgcaaa
    78421 catccaaatg agtggttgcc atgttaattc ccgggctgat ttatcgattg ttttgccccg
    78481 cagactgtgc gcgcttcgac gcgtcaggca ccagacgata aaaagtttcg cccggcctgg
    78541 tcatgctgag ttcattacgc gcacgctctt cgagcgcctc ctggccgcca ttgagatcgt
    78601 caatttcggc aaaaagttga tcgtttcgcg ctttaagttt cgcgtttgta gcttgctgtg
    78661 ccgccacatc atcattgacg cgggtatagt catgtatacc gttcttaccg aaccacagcg
    78721 aatactgtag ccagaccaga atagccagca acagcagcgt tagtttaccc atcctgcccc
    78781 ctgaaaaacg gcatcatcat cccatgcatc cgaagacgac tctacatcct ctgttgggga
    78841 taccgcgaca acgcgggcaa atgtaccaca tttgtccatt gttacgtata cccagggcgt
    78901 gcagaacata atctcattat tagttacggt ttgaattatg aacagaggag acgggaaagt
    78961 acaaattagc ccagtagcca cataaacagt gcgccaaaca taatgcctac tgtcaccagg
    79021 gtgaaaacaa tactgtagcg tagctttccg tccatcaatg aatgcagcgc aatccccacc
    79081 actaccgcga cgggcatcag cgccagaaag aaaggccagg tgtagataaa gaagaacagc
    79141 gtgttagagc cataaatcaa catcggcatc gccagcgcaa ataaccagga gataaaaccg
    79201 accacggcac caggcagtga ccatgtggtt tcttcatcct cagtaaggct gtcgttattt
    79261 gttagtgtaa tgttatggct attacgcata tttgatcctg ttactttgac gaaccgggca
    79321 tggaaacccg gtggtgtctc aggatctgat aatatcgttc tgtctcaaca gatctaataa
    79381 ttgctgtacc aaatttgtta ctaattgttc accattgaga tgaatttctg ccgattcagg
    79441 cgcttcgtaa acagaatcta ttcccgtaaa gttgcgcagt tcaccggcac gcgctttctt
    79501 atataagcct tttggatcgc gggcttcgca aatcgccagc ggcgtatcga caaacacttc
    79561 gataaagcgc ccttctccta cgcgttcgcg aaccatctgg cgttcggcgc ggtgtggcga
    79621 gataaatgcg gtcagcacta ccagtccggc ttcaaccatc aaattcgcca cttcaccgac
    79681 gcgacggata ttctctttac gatcggcatc gctaaaaccg agatcgctgc ataatccgtg
    79741 gcgaacatta tcgccatcca gcagatacgt actgacgccg agtttatgta acgcctcctc
    79801 cagcaccccg gcgaccgttg atttaccgga cccggagagg ccggtaaacc acagcactac
    79861 accacgatga ccgtggtgta gctcgcgttg ttgcacagtg accggatggc tatgccagac
    79921 gacgttttcg tcatgcagcg ccattattta tcccccagca aatcgcgcgc gccccagtgc
    79981 ggaaagtggc gacgaaccag agcattcaat tccagttcga atgcactgaa ttcagatggc
    80041 gcagcagttg cctggctaac tggctcgtgc accataccgg cacccacggt cacattgctc
    80101 aggcgatcga taaaaatcag cccacccgtc accggatttt gttgataacg atctaacacc
    80161 agcggctcgt caaaagtgag atccacgagg ccgatcccat tcagtggcag gttttcaact
    80221 tcacgctggg taaggttatt aatatcaacc tgatagcgaa tgccatcaac acgcgcgcgc
    80281 gtcttcttac cggcaatttt gatgtcgtaa ctctgccctg gagaaagcgg ctgttccgcc
    80341 atccatacca catccaccga cgcgctctgc accgccggta acgcttcgtc tgccgccagc
    80401 agcagatcgc cacggctgat gtcgatctca tccgtcagca ccagggtgat cgcttctccg
    80461 gcaaaggctt cttcgcgatc accatcaaaa gtcacgatcc gcgcgacgtt tgattccaca
    80521 ccagaaggca gcacttttac acgttgcccg acttccacgc gaccggatgc cagcgttccg
    80581 gcgtaaccac gaaaatcgag attcgggcga ttaacgtact gcaccgggaa gcgcattggc
    80641 tgagcatcca ccactcgctg gatctccacg gtttccagca cttcgagcag tgtcggaccg
    80701 ctgtaccacg gcatactttc actttgcgaa gccacgttgt cgccttccag cgcagagagc
    80761 ggcacaaagc gaatatccag attgccaggt aactgttcgg cgaaagtcag gtagtcttta
    80821 cgaatgcggg tgaatgtctc ttcactgtaa tccaccagat ccattttgtt gatcgctacg
    80881 accagatgtt tgatccccag cagcgtggag ataaaactgt gacgacgggt ttgatcgagc
    80941 acgcctttac gggcatcgat cagtaagatc gccagctcac acgttgaggc gccggtcgcc
    81001 atattgcggg tatactgctc gtgcccgggg gtgtcggcga taataaattt acgcttctcg
    81061 gtagagaaat agcggtaggc cacatcaata gtgatgccct gttcgcgctc agcttgcagg
    81121 ccgtccacca gcagagccag atccagtttt tcgccctggg tgccgtgacg cttactgtcg
    81181 ttatgcagcg atgagagctg atcttcgtag atttggcggg tatcgtgcag caggcgacca
    81241 atcagggtac ttttgccgtc atcgacgcta ccacaggtca gaaaacgcag caggctttta
    81301 tgttgttgcg caatcatcca ggcttcgacg ccgccttcat tggcgatttg ttgtgcaagt
    81361 gcggtgttca tcttaaaaat acccctgacg ttttttcagc tccatagacc ccgcctggtc
    81421 gcggtcaatc acgcggccct gacgttcact ggtggtggaa accagcatct cttcgatgat
    81481 ttccggcagt gtttgtgcat ttgactccac cgcaccggtc agcggccagc agcccagcgt
    81541 acggaaacgc accatccgtt ttttaatcac ttcgcccggt tgcaggtcga tacggttgtc
    81601 atcaatcatc atcaacatac cgtcgcgttc cagaaccgga cgttccgcag cgagatatag
    81661 cggaacaatg tcgatatttt ccagccagat gtattgccag atatcctgct cggtccagtt
    81721 agagagcggg aagacgcgga tgctttcgcc tttgttaatt tgcccgttgt agttgtgcca
    81781 cagctccggg cgctgatttt tcggatccca gcgatggaag cggtcacgga aagagtaaat
    81841 tcgctcttta gcgcgggatt tctcttcgtc acggcgcgca ccaccgaagg cggcatcaaa
    81901 accgtatttg ttcagcgcct gtttcaggcc ttcagttttc ataatatcgg tatgtttcgc
    81961 gctgccgtgc acgaatggat taatccccat cgccacgcct tccgggtttt tatgcaccag
    82021 cagttcgcag ccgtaggctt tagcagtacg atcgcggaac tcatacatct cgcggaattt
    82081 ccagccggta tcgacatgca gcaacgggaa aggcagcgta cctggataaa acgccttgcg
    82141 cgccaaatgc agcatgacgc tggaatcttt accgatagag tagagcatca ccggatttga
    82201 gaattctgcc gccacctcgc gaataatgtg gatgctttcc gcctccagtt gccgcaggtg
    82261 agtaagtcgt ttttgatcca taaccgttcc tttgcaatac cgctattttc ttgccatcag
    82321 atgtttcgac tatagggagc gtaagagaac gaatgaaatt accaattaga atgagtagtt
    82381 ccttaacgga ataacgattt ggcaaagcta atatcaaaaa gtgcttaagg caccggattt
    82441 cgggcgttta ggaagatttg aaattgtttt agcgcagcgg cagtttcata ctatggcggt
    82501 aaaaaaattt gcatggtatt taaggactca ctatgttttc cgcattgcgc caccgtaccg
    82561 ctgccctggc gctcggcgta tgctttattc tccccgtaca cgcctcgtca cctaaacctg
    82621 gcgattttgc taatacccag gcacgacata ttgctacttt ctttccggga cgcatgaccg
    82681 gaactccagc agaaatgtta tctgccgatt atattcgcca acagtttcag caaatgggtt
    82741 atcgcagtga tattcggaca tttaatagtc ggtatattta taccgcccgc gataatcgca
    82801 agaactggca taacgtgaca ggaagtacgg tgattgccgc tcatgaaggc aaagcgccgc
    82861 agcagatcat cattatggcg catctggata cttacgcccc gctgagcgat gctgacgccg
    82921 atgccaatct cggcgggctg acgttacaag gaatggatga taacgccgca ggtttaggtg
    82981 tcatgctgga attggcagaa cgcctgaaaa atacgcctac cgagtatggt attcgatttg
    83041 tggcgaccag cggcgaagag gaagggaaat taggcgctga gaatttactc aagcggatga
    83101 gtgacaccga aaagaaaaat acgctgctgg tgattaatct cgataactta attgttggcg
    83161 ataaattgta tttcaacagc ggtgtaaaaa cccctgaagc agtaaggaaa ttaacgcgcg
    83221 acagggcgct ggcaattgcg cgcagtcacg gaatagccgc aacgaccaat ccgggtttga
    83281 ataaaaatta tccgaaaggc actgggtgtt gtaatgacgc agaaatattc gacaaagcgg
    83341 gcattgctgt actttcggtg gaagcgacta actggaatct tgggaataag gatggttatc
    83401 agcaacgcgc aaaaacagcc gcattccctg cgggaaatag ctggcatgac gtaagactgg
    83461 ataatcaaca gcatattgat aaggcacttc ctggaagaat agaacgtcgc tgccgtgatg
    83521 ttatgcggat aatgctaccg ctggtgaagg agctggcgaa ggcgtcttga tgggtttgaa
    83581 aatgggagct gggagttcta ccgcaggggc ggggaattct aagtgatatc catcatcgca
    83641 tccagtgcgc ccggtttatc cccgctgacg cggggaacac aaaaccaaac ttctccataa
    83701 attccatagc cgcggtttat ccccgctggc gcggggaaca cgagtctatc agcgacacta
    83761 ccggcaatag cgacggttta tccccgctgg cgcggggaac acctatagcg ccacgttccg
    83821 agcgctgcga gctgcggttt atccccgctg gcgcggggaa cacctaatga gtcagtttga
    83881 acagatcccc gacaacggtt tatccccgct ggcgcgggga acacggcttt aaaattgtgt
    83941 tcccgtctgg cgctgggggt ttatccccgc tggcgcgggg aacacgcatc catgccgacg
    84001 cctttacgtg tgcggggcgg tttatccccg ctggcgcggg gaacacgcgc gaatttgtgc
    84061 gcatggggcg catttttggc ggtttatccc cgctggcgcg gggaacacac gatggcgatg
    84121 cgtgagaaag ggggtcgata cggtttatcc ccgctggcgc ggggaacaca gttcgctgag
    84181 tagccttttt tctgtgccta acggtttatc cccgctggcg cggggaacac gaggtggcaa
    84241 tacgcgtaga tcatttggtc gtcggtttat ccccgctggc gcggggaaca ccaaaatatt
    84301 acgagcttcg tcaggccatg gaccggttta tccccgctgg cgcggggaac acactaagca
    84361 tacataactg tttttaaaca aatttattcc gcatcaacaa tctaccaact aaattaaaac
    84421 atttccttat ttttaaagaa cggataactt attgattatc aacaggaaga aaagaaacca
    84481 aacgtaaccc atccaaatcc accggaatac gtctgttttc tccccaggtc tgaaattcaa
    84541 aacccgactc ggtattggtc gcccaggcca tcaccacatt tccgcaacca gccagttggg
    84601 taatttgctg ccagatcatc tcccgaatac gttttgatgt atcaccaaca tacacaccgg
    84661 cacgcacttc cagtagccag attgcgagcc gtccacgtaa gcgcggcggg acattttctg
    84721 taacaaccac gaccatgctc atcagccgcg tcctcgatga ccgctatcgc ccaacgtttc
    84781 aggttcgggg atggctggcg gtaacatatc cggcgcaggt tgtggcggtt caatttcacc
    84841 tgcggcaagg acttcctcaa ttaacggtat taatttgccc gttaacttag tgctacggaa
    84901 aatatcgcga caggctaatc tgacttcttt atcaggttct gcgggttgcc tcgctgctat
    84961 ttcaaatgcc tttggcacaa ccgaatcaaa tttaatgata tcggctatgt cataaacaaa
    85021 tgaaagcggt ttgccactat ggataaaccc aatagcgggc gcatatcccg cggctaatac
    85081 tgccgcttca gatataccgt acagacatga tgtagcagca ctgatgcagc gattcacaac
    85141 atcgcctttt tcccagtctt taggatcgta tttgcgacca ttccatttca caccatattg
    85201 tttcgccagt aatgcatacg tctggcgaac gcgagatccc tcaattccac gtaactgctc
    85261 cactgaacgg cgagctggct gtggttcacg aaaacgtaat tcatacattt tgcgcaccac
    85321 cttcaaccgt agatcttccg ttaaagccag ctttgcctgg tagagtaatt tatctgcccg
    85381 cgccccaccg ggttgtccgg aggagtaaac gcgaacgccc gcttcaccga cccagaccag
    85441 cagtgttccc accgtggcgg ccagatgcac cgccgcgtgg gaaactctcg ttcctggttc
    85501 gagcataatg caggcgaccg atcccaccgg aatatgcgtt cggatccctg ttttgtcgat
    85561 cagcacgaaa gcgccgtcca gtacgtcgat ctgaccgtac tggaggaaga tcatggaggt
    85621 gcgatctttt aatgggatcg ggctcagtgg tacaaacgtc acgcctctgc tccgggtttg
    85681 atcagcatca gaccacagcc gaacgcccgg cttttaccat acccctggct caggcgttgc
    85741 acaaataacc caggatcggt gaccgtaagc atccccgtat aatccacgct actgaactgg
    85801 atcagttgcc gggagttttc ccgccgcagt tgctgttgtc ggtaggcatc aaccgaggta
    85861 tccagcagtg taaaaccgct tctctccccc tgcgctgcca gccagtccag cgccgcctgt
    85921 tgttgatgca accagacatc acttccttcc gcctgccccc tcacctgccg tttcgcctcc
    85981 atcagcagat cgtggcgctt gcccgcttta cagattgttg gattcgcccg caggttgaaa
    86041 caaagttgtt gtccggtaca cagctcgggc gcaaatgacc ggcattctat ggtgaacgta
    86101 tcactttccg ccgggcgttc ctgcgacagt acaaaaaagc gaaacgcgcc ctggagttct
    86161 tcccgacgat aaagaaattg cctttctttg ccgccaggga agagatccca cagccactga
    86221 tgcatcacat attcaccgcg atccaccaga tgcagcaact gcgcaggcga aagctggccg
    86281 gtatgtaagg ttattcttga gaggtacatg gttcctcctt gctgagccac ggcccctgat
    86341 tgacggtgcg ttccccaaac agccactgct gacgatttaa aggaacatcc cggcggcgta
    86401 atattttgct cgcgaccagg ccgtcgtgtt tcccttccca ccagcattca tcctgaagtt
    86461 tcgggagtga gactttcagt ttatggaaat gatcctgata ctgttggtat gcattacata
    86521 aggcatcaca cgcgttgcct tcgagcagta acggcgcaag aggtaatgcc agcggatgac
    86581 tttttcgccc cagataaagc ggaaaaactg gatggcgtaa accgtcctgc aactgttcga
    86641 ggctgtaagg cgcatcggct gttgttgcca ccgccaccat ccaccaggca tcggtgtagt
    86701 agtcgcgccg ggagataatc gcgctcaaaa gctcagggtc gctcaactct tcgcgacggc
    86761 tgaaataacg tgctttacgc acctcttttg gcatctggac cgtgtgataa tcccgtgccc
    86821 agcgcgggtt acggctggcg caaaccacca gtgaatagtg gcgattaaac gcgtttaagc
    86881 gttcggtatc atcacgccga atccctaccc cggcagccag tagccccagc aatgctgagc
    86941 gcgaaggaag ttcatgggta tgacgaactt cacctggagc atcgaccccc caggacgcca
    87001 tcggcccatg aagctgaaaa atcagatatt ggctcataag ccgcccctta cgcgcagata
    87061 aagtccagca cgtccttcat gcttccctgt ttgttcatca cgtcaaaact tgcgcattcg
    87121 gtcttctgtt cgtagaccgt attcatgttt tcgcgaagcg ttgtaatacg ctgcaccgcc
    87181 acatctaact gacgtgtgcc attaatgggt tcatagaaag ccgccgccag agagcgtggt
    87241 tgatcggtgc ctttttctgc cagcgcccag gaggcgtagg cacggctggc aaagctgttc
    87301 tgtttgccgg ttggggagac tttaagtgcg gcttccgtaa aggcacgtat ggtctgatta
    87361 gctaacgctt cgtcaccgcc gaggttttct accagcagat ctttatcaat gcagatatag
    87421 gtgtagaaca gcgcagaacc gaatccggtt tcatctacat gaccggcacc ggcatcttca
    87481 gaagcctggc gtaaatcatc tacggcggtg aaaaaatcat tttcgacaat cgtttcactg
    87541 acaccaaacg catgcgcgac ctggcaggcg gcttctacat taaacccggg ttttttcgcc
    87601 agcatacgac caaacatagc gatatctacc gccatacggt ctttacgtaa caaagcgaga
    87661 tctttctctt ttggcgcgcg cttttcttct gccagttgat gggccagcgc ttttacggcg
    87721 tcaaattctg ccgggctgat atggacgagc tgttcagttt cggcgctagt gagcggatct
    87781 tttggttttt tgtcgttttt ttgttttttg tcttttttgg ctttcccaag ataatcggct
    87841 atctctactg cccattcgat ggctttttta tcttcgattc ctttctcaat caggatagtt
    87901 gccgcctcac gcgcaatacg cccgctacga acaccaatat ggcccgccag cgcctgttca
    87961 aaaagttcag aagtgcgcca agcacgcttc agactttgcg aggaaacgcg cagacgcgtt
    88021 gctccaccca gaatcacagt tttcggagag ccggaatcat cacggttaag attggcagca
    88081 gggtaagcgg ttaacaaatg aagctgaata aatgttgtca tgagatagtc ctttattaat
    88141 tttgttcgtt atcggcgtcg ccagcctggt aatattccag cgcccagcgg atacggataa
    88201 attctgttgg tcgctgctgg cggcggtgat gattgagcag gtcatcgctc tcctggcacc
    88261 agcggaagac cccctctgcc agagagtcaa gattgacgga accgtttaat aatttgaccg
    88321 cacggcgtaa ctgccgcaat aaatcatccg gcgtttttac tgccaacaaa cgagtaaacc
    88381 gccccttttt cattaccgcc gccagttgcg cggcaaaagg ctgccgttcg tcaatagctt
    88441 taacattggc gctaactgct gctatcagcg ctagtgcggt gatacgccac tcgggttcat
    88501 cctgccactt tatttgtctg ttcttaagaa acaggcgaaa tccatccgtc agacaaacat
    88561 cattaaccgt cgtactacgc cgcagactgg cacgctcgcc gcgtttatcc tgcaactctt
    88621 catgccattt acgcagcgca actttatgct catcttttac aatactcatt cagcagcctc
    88681 ctgctttttc tccctggcgg cgttagcatt ttgcttctcc gctgatgttg taaaatattt
    88741 cttgcgtgcg gtcatgacgc gttccaaatc aacgggctca tatggattgg taaagacacg
    88801 atcgtcaaaa tcctgacgcg cgaataacca catttccttt tgccatttac tgagtaattc
    88861 gtacggatcc tgaccttctt caattttgcg cactaatctc aggaatcgat gctgagtttt
    88921 gttccagaag tcaatatcca caaaaccgaa atcaagcttt gtggttttct tctcagagaa
    88981 ccatgcttct ttcaacgctc ggtgtaacaa gcttagaatg tgtgatgccg cttgcgctgc
    89041 cagacgcaac ttaggtatta ggtcttcttt tttgtttaac aacagcggga aatggtgttc
    89101 ataccaacaa cgaaccttca tatcttcaaa atcaaaacca aatccccata agcccaccct
    89161 tgtctgcttt agattgctgg cattcaaaag tttcacgacc tgtgcgggga gttcagtatt
    89221 gttttttgag ttacctacct ctatgaggcc taaccagtcg cgccagatta aaccaccagg
    89281 ttgtggttta actgagtaaa aatctccacc ctctttaagc ggcagacggt aaggtgttag
    89341 aggatggcgc cacatgacgt attgaacacc atagtttttg agtgacatca agcccaaaag
    89401 agcatcgctc tgcctaccgc aaatgtcgca gttacctatt gaggttgtct taaaatcaat
    89461 acgtatacgt cgtggcattc cccagtaggc ctgaagttta ttgacttgtt catccgttac
    89521 cacagcaccg tccagttcgc tggtacgcgt cggtgcaagc catggaaaga ccaggtcatc
    89581 aaaggttttt ggtaaaggta aatcggcttc atcttgcggc attacgttta gccacagctt
    89641 tcgccacaaa ggggtttgtt gattgccctg atactccagc aattctataa gtgtcgtcag
    89701 aggccctccg ccacgtagcc caggatagta accgcgtcca cctataggtg cattcaactg
    89761 gattgaaaaa agcgctaaag gcacacaatg caggcacaga tgctgagttg tgtctcgctt
    89821 aataaaatgg tctgtatttc gttcttttgt ttgttttcct ggtgcgtcag gaagaagcga
    89881 agcaatagaa gttgccttaa cttttaattc atcaaaatcc tgcataaatg aaggggaatc
    89941 tggcccaaac tgaaaagcat gttctaaaga cagcaatgct tcccttagct tttcagcttc
    90001 cagtccgtct tcccagatat catcccaacg acgttgatct tttggcgcga agctggtttg
    90061 tagtagcccc aacaaaaact gccaggccgc cccctggaga tctgcccgcg gcgcagcgat
    90121 atcgacaaca ttttcatcag ccagatcgac tggtgccagt ttgcctgtcg ttccgtcttt
    90181 aaaacgaacg ggcaaccacg gggttgtcag aagtgaaaac gagttcatac gcatcctctc
    90241 gtaaaaaaga ccatcccagg tcggttgtcc atccttccat ccttttctgt atatcaacta
    90301 ttcagagagt cctgtcgcag acatctcaac caaccttgcc aatcaatccc tccctggccg
    90361 aatacccgca actttcgtca tcggtgacta aaatcacgtt tgccatttcc ggatcttgcc
    90421 gctgttcaac gcaccactgc ctgaacgcat ccccttccag taaagaaaac tcatcccgat
    90481 gttttttcca ccagcttcgg cgcactctga caacgctcat ttcccatgcg tgagcaccgg
    90541 tggcataagg cttcaccaca ccgtcaatac aggtagccag ccacagggaa acagactctt
    90601 cagccagacg tgtcgacagt ttttccggaa ggtaatcgtt gatattggcg gcatagccgg
    90661 gcctgaagtt cagcacatac tttttagccc ttgcgcgatc acagtaatat ttgcccactt
    90721 gctcctgctc gctgcgggca aatccttccg gcattaccac gtcctcaccg taaactgatt
    90781 caataagaag ccgggctgcg tgtggcatct gaatagcgcc ttgctcacgc agcacacgct
    90841 gcgtcagcca gattcgccca tgatcgggat agacatatgc actgttacgc atggcactgc
    90901 cgaaccattc gtcaccagga gcgtcatccc agacgggggc cagaatcagc aattcagggg
    90961 gggaacgctc gtcttttccg tcacgcttta actgaccatt aatatcgcgg atatgccgct
    91021 gtaatcgccc cgctcgctgg attagcaaat caatgggggc caagtcggag atcatttcgt
    91081 ccaggtcaat atccacgctt tgttcaatta cttgtgtaca aacaatgact tgtgacgctc
    91141 tctgtaaact acaatattta ccaaagcgtg ccagcgtttc cgtttcaatt cgctggcgat
    91201 cgctaaaagc aaagcggcta tgaaaaagtg aaaggctgga agcaggaatg acgccgcggg
    91261 caagcagctg acgatacacc tgaatagcgt catcaacaga attccgaatc caggcgatgc
    91321 atttcccctg acttaccgcc gattcgatac gcgcaataca ctcttgttca ctatggagcc
    91381 aacccacgct cacgctacgt tcaacttctt tgcgcgtcgc tacccggtgc gaatgtacat
    91441 cggttttcgt gacatgcgtc agccaggggt aatcatcttt tcccaggagt ggggcttctt
    91501 gctggccctc tgcaccacgc gcaaaggcgg cgacgagttt gtcgcgctgc tgttgagata
    91561 acgtagcaga aagcaaaata acgctgtttc cgccacgcgc ctgccgctcg atcagccctt
    91621 caagaatgca cgacatgtaa gcgtcacagg cgtggatctc atcagccagc aggattttgt
    91681 tactcagccc cagaagccgc agattattat gtttaaacgg catcactgcc atcatcgcct
    91741 gatccagggt gccgacgcca atttcagcca gcaacgcctt cttgttactg tcggcaaacc
    91801 aggccgcaca accctggctg aaagcttgtt catccggttc ttctgacccg attaaatcac
    91861 cggaccagag tgattcatta aagcggtcca ttaatgtgcg ggcactgtgt gccagcacca
    91921 ggctggggcg agattctggc gaatagaaag aaagccaggt tttgaccagc cgatcgtaca
    91981 tggcattggc cgttgccatt gttggcagac caaaaaacag cccctgagct ttccccgcag
    92041 ctatcaacct gtgcgccagg ataagcgccg cttctgtttt acctgcgcca gtcacatctt
    92101 ccagaataaa taactgcggt cctggctggc tgatatccag atccagcacc ttttgctgta
    92161 acggtgtcgg attctcaata aaaggaaaca gtgtattaat tccggtgaaa ggtgcggttt
    92221 ctgcttttgg aggaaagacg gttaatgcgt tttgagcctg cactaaagcc ttctgccagt
    92281 aatgtttaat atccattgcc tgtgcgacgc gtggaaaaaa tcgcgttgac gaacctgtcc
    92341 agtctgcgag tacgacggtt gcagagatat accaggaaag gtgttttata agttcaatgc
    92401 cctcgtcatc atcccagaat gcggggatct ctattcgcgg aaacagtgcc ttgattgcaa
    92461 ggagaaaatc tcgtgctgca gctttgtctt caggcagaaa attatccaat tcatccatac
    92521 gatcaggggg ccgaccatga tgccctgtcg tcacgggcat ccacatttct atcactcgcc
    92581 taagtttgcg cggagagagc gaagatgaag gaagtaactc ctgacattca cttaaataat
    92641 gattccacaa ccagtagccc agcgttgaat gagagatttt ttcgtaattt tttctggcac
    92701 tttcctgaat cttgagttca gggggcaggt aaagttgttg aaaagagcga gcaaattttc
    92761 caatatcgtg ccagcacagc aaccaggcga aaaattgagc cgcctgttcc ttgtcagaaa
    92821 tccctaattg acgaaaataa tcagccagcc cgaagcaatt tcttttaacc attaaatagc
    92881 ccatcgcggc tacatccagc gaatgccagc aaagaaggtg atagtcgtcg cccccgtctt
    92941 tctcgccgcg tcgggttttt ccccagaaat caaaaaaagt cacaatattt ttatccttca
    93001 gtaaacttaa aggatattta cgcatatttt aaaaattatc tgtgatatat atcagccaat
    93061 aaaacattta cattatgaat aaattaatga ttttcatttg aaattcataa taataaatag
    93121 agactatata ttcacataaa aaatataaat tttaaaaaag ggtgcaggag accaaaccat
    93181 cacaattcag tacgcggggt tttacccccg cgtggtggct acttcttcga ttcgtaagcg
    93241 agaactgcct taaactccat accacgttct ctgatactca actcacacaa cgagtcacct
    93301 accatcagcg tgaatcccag tactgtacaa cacagtacga tgattgccac aagggcatat
    93361 tttgtcagca tgtctccctg caaagaagag actaacattc gaattgtttg ggttcgaacg
    93421 taagcctcag gttgatagaa atatcgcctg gggcttttgt ccatctggaa cctcgcgaat
    93481 gcttaacgcc agacagcctc aagcacccga cgccattcta tacctgataa ttcttccagc
    93541 ggcgttcacg tttaaaatca ggaatactgc tcgcaaaata aaaacgcccc catcatttct
    93601 gacagaggcg tttaatttat ccggcaatat ttacccttcg tgcaatccac attccctttt
    93661 taagccaaag aaacgtgttt cttcttccga catgccaggt tcccatttac gggttgtatg
    93721 ggtatcgccc accgataaat atccttcgtc ccataatggg tgatatttca ggccatgttt
    93781 ttgcagatac tgatatatcg ttcggttatc ccagtcgata atcggcagca ctttaaatac
    93841 gccacgctga attgccagca ccggtaaatt ggcgcggctg ccggattgtt cgcggcgcag
    93901 gccagcaaac cacgtttgcg cattcagttc tttcagcgcc cggttcatcg gttcgacttt
    93961 gttgatttca ttgtactttt caatgccttc aacgccctgt tcccacagtt tgccgtagcg
    94021 tgcttcctgc caggcggcgc tttcggtagc gcggtacact ttcaggttga gcttgagttt
    94081 gtccgttaac tcgtcaataa agcggtaggt ttccgggaac agatagccgg tgtcggtgag
    94141 gatcaccgga atatccgggt gaatctgatt caccagatgc aggctcaccg ccgcctgaat
    94201 gccaaagctg gaagaaagta catattcacc gggcagatta tccagcgccc aggcaacgcg
    94261 gccttcagcg tccagttttt ccagttcggc gttagtttct gccagcgcca gaatgcgatc
    94321 taccttcggc agttcgttca gggcgtttag atcgagtttg gacatagatt cctcactgtt
    94381 tgccttgcct gatgcgacgc tcacgcgtct tatcaggcct acaagaccgg gctgatggtt
    94441 aatcccacaa atcgcgcgcc ggatcgagca ccgggcgaat gatgcccgca cgcaccgtaa
    94501 agtcgccgaa gccttcaccc gcttcgcgct ctttcgccca gcgccctatc agttcatcaa
    94561 gcgacgccag gatttccggc tcggtgatgt tttctttata catccgtggg atacgtgtcc
    94621 caatgcggtt gccgccaaga tgcagattgt agcgacccgg tgctttaccc accaggccca
    94681 cttccgccag catcgcgcga ccacaaccgt tcgggcagcc tgttacacgc atcacgatat
    94741 gctcatcgct gacaccatgt ttcgccatta aattatcgat gttgtcgata aaagacggca
    94801 ggaaacgctc tgcttccgcc atcgccagcg ggcaagtcgg gaatgacacg caggccatcg
    94861 agttttcacg ctgcggcgtg acggcattca ttaaaccgct ctctttggcg atcttctcga
    94921 tcttcgcttt ctcgctttcc ggtacaccgg cgatgatcag attctggttc gccgtaatgc
    94981 ggaaatcgcc tttgtggatc ttcgcgatct ccagcaggcc ggttttcagc ggacgccccg
    95041 gataatcaag gatgcgacca ttttcgataa acagcgtcag gtgccagtta tcatcaatgc
    95101 ccttaaccca gccaatacga tcgcctcgtc cggtgaactc atatggacgg atcggttcaa
    95161 atttgatccc cgcgcgacgc tccacttccg ctttaaacgt ctcaaccccc acgcgctcca
    95221 gcgtgtattt ggttttggca tttttacgat cggttcggtt accccagtca cgctgagttg
    95281 tcacgacggc ttccgccacc gccagcgtat gctccagcgg cagatagcca aactcgctcg
    95341 ccgtgcgggc gtaggttttc ttgttgccgt gttcgataga aagcccaccg cccaccagca
    95401 ggttgaagcc taccagcttg ccgttttcgg cgattgccac gaagttcatg tcgttggcat
    95461 gcagatcgat atcgttctgc ggcgggatca ctaccgtggt tttgaactta cgcggcaggt
    95521 aggtctgacc aaggatcggt tcttcatcag tagtggcgac tttttcctgg tcgagccaga
    95581 tctccgcata cgcgcgggta cgaggcaaca gatgctcaga aatcttcttc gcccactcgt
    95641 acgcttccgc gtgcagctgc gactcgtaag ggttcgaggt gcagagtacg ttacggttca
    95701 tgtcgttagc tgtcgccagc gcatcaagac cgaccgagtg cagcatctgg tgcaccggtt
    95761 tgacgttctt tttcagaatg ccgtggaact gaaacgtctg gcggttggtc aggcgaatgc
    95821 tgccatagat ggtgttttca ccggcaaatt tgtcgatcgc ctgccactgt ttagtggtaa
    95881 tcaccccacc cggcagacga cagcgaagca gcatcgcgtg gcgcggctcc agcttctgtt
    95941 cagcacgttc ggcgcggatg tcgcggtcat cctgctgata catgccgtgg aagcggatca
    96001 gcaggaagtt atcgccctta aagccgccgg tcagaccgtc gtttaaatct tccgcaatgg
    96061 tgccgcgcag gtagttgctt tcaagcttca tgcgctcggc gtctgtcagt tttccttcga
    96121 ccactaaagg ccctggatgt ttttcgctca ttagtagaca tctcgctgat aacggcgctc
    96181 tacgcgcagc tcacttaaaa attcatccgc cgcttcggtg tccatgccac caaattcggc
    96241 aatcacttcc agaagtgcct gctcaacgtc tttcgccatg cgattagcgt cgccgcagac
    96301 ataaatgtgg gcaccatcat tgatccagcg ccacagctcc gcgccctgtt cgcgcagttt
    96361 gtcttgtacg taaacttttt ctttttggtc gcgcgaccag gcaagatcga tacgtgtcag
    96421 cacgccatct ttgacgtagc gctgccactc cacctggtac aggaagtctt ccgtaaagtg
    96481 cggattacca aagaacagcc agtttttacc tggcgcttcg tcggcggcgc gttgctgcat
    96541 aaaggcgcgg aacggcgcaa taccggtgcc tgggccaatc ataatcaccg gggtttccgg
    96601 gttagcgggc aggcgaaagt tatcgttatg ttcgataaat acgcggactt cgccctcttc
    96661 ttccacgcga tccgcgagga agctggaggc accaccggca cgggctcggc cttccacgtc
    96721 gtaacgcacc acaccaacgg tgacgtgtac ttcgttctcg acttccgcct gcgaggaggc
    96781 gatggaatac aggcgcgggg tcagcgggcg cagcagatta atcagcgctt cggcatccag
    96841 ttgcgccgga gagaaacgca ccatgtcgac aatcggcgtc gtcgcggcgt aatgctgtaa
    96901 cttcgcttta tcgcccacca gcggcagcag tgtttcactg cgggtaagcg tggcgtaatt
    96961 ctcaacaatg ttggcggtgt tgacggtcag ttcgaagtgc cactgtagcg cttcgttcag
    97021 aggcaacgtt ttgccctcga cggtgacagg ttcatcgcct ttcagccaca gcagttcgac
    97081 aagttctttc accagtgccg gatcgttctg ataccagacg cccagcgcgt cacccggctg
    97141 gtaacgcagg cccgagtcac ctaagtcaat ttcgatatgg cgaacgtctt tttcagagtt
    97201 acgtccggta attttctggt taaccgaaag gctcgccacc agcggagcgt ctttgctgta
    97261 cgggctggag tggatttcat ttaccgcgcc agtagcgacg gattgcgaag gtgccgcgac
    97321 aggcgcacgc gatttaagcg catcaaccac gcgggcgcgc cactcgctgg cagcagcctg
    97381 gtattcaaca tcggcatcga cacggtcgag caggcgttca ccgcccagtt ccgccagctt
    97441 gctgtcaaaa tctttcccgg actggcagaa aaattcataa gagctatcgc cgaggctaaa
    97501 cacggcaaat gcggtgtttt ccagcttcgg cgcttttttg gagaacagga acttatgcag
    97561 cgcgacggct tcttccggcg gttccccttc tccttgcgtt gacgtcacca cgataagcag
    97621 tttttcgctg gcgatttgtt tgaatttata gtcgcccgcg ttcaccagct taacgttcag
    97681 ttttgctgct aataaatcat cgcgtaatgc ttcagcaacc cggcgcgcat tgccggtttg
    97741 cgaggcggag ataatagtta tacccggcat ttctgcggct ggcgctggcg tcgctgcaag
    97801 cgcagcaggc tgctgattga gtacgcccca gaaatagcca gaaacccagg caagctgggt
    97861 gggagttaaa tcggtcgtgg ccgcctgaag gcgtgccagt tgctccgggt tcaacggaag
    97921 caacgcggaa ggtgggacct gtgtcgtcat gcgtcgttat gttccagtaa gcaaagctgt
    97981 ttctgcgccc tgtcagcgcc cataaaacag aagagaaggt aaggttaacg gggtgaacgg
    98041 tgtggattaa agacgggata gcgataacta ataaccaaat cgactaacct gttttagcaa
    98101 tagtctttaa caacaaaata gattaaccaa cctaatgaaa aacaaatgaa tttagccaat
    98161 cattaagata aatcagcgat tttgcgcaac aaagtcgttt tagataatgc gaaaaaacag
    98221 cctttccggt actctacggc ggttttatcg tcctgtagag aaattatgat gtccaccacg
    98281 ttatttaaag atttcacctt cgaagccgct caccgcttac cacacgtccc ggaagggcat
    98341 aaatgtggtc gcctgcacgg gcattccttt atggtgcgac tggaaattac cggggaagtc
    98401 gatccgcata cgggctggat tatcgatttc gctgaactaa aagcggcgtt taagccaact
    98461 tatgagcgcc tcgatcacca ttatctcaat gatattccag gtctggaaaa cccaaccagc
    98521 gaagttttag caaaatggat ttgggatcag gttaaacccg ttgtgccgct gttaagtgcg
    98581 gtgatggtaa aagaaacctg caccgctggt tgtatctatc gcggcgaatg ataagagtgt
    98641 gtcggcggtc aatttccctt aagtaacgct atgttagggt gttgtgttct ggatatctgg
    98701 ggcatgacat ggaagacgac tgcgacatta ttattattgg tgccggtatt gcaggcaccg
    98761 cttgcgcgtt acgctgcgcg cgagcgggtt tatccgtttt gttactggaa cgcgctgaaa
    98821 tccccggcag caaaaatctt tccggcgggc ggttatatac ccatgcactc gcggaactcc
    98881 tcccgcaatt tcatctgacc gcgcctcttg aacgacgcat cactcacgaa agcctttccc
    98941 tgttaacgcc cgatggcgca acgacgtttt ccagcttaca gcccggcggt gaatcctgga
    99001 gtgtattacg tgcacggttc gatccgtggc tggttgccga agccgaagaa gaaggtgtcg
    99061 aatgcatccc cggagcgacg gtggatgcac tgtatgaaga aaacggcagg gtgtgtggtg
    99121 tcatttgtgg tgacgatatt ctccgtgccc gttatgtggt gctggcagaa ggtgccaaca
    99181 gcgtcctggc tgagcgtcac gggttagtga ctcgtcctgc tggtgaagcg atggcgttgg
    99241 ggatcaaaga agtgctgtcg ctggaaccat ccgctattga agaacgtttt catctggaga
    99301 ataacgaagg cgcagcgttg ctgttcagcg gcaggatctg tgatgactta cccggcggcg
    99361 catttcttta tactaatcaa caaacgctct cgttagggat tgtttgcccg ctctcttccc
    99421 ttacgcaaag tcgtgttccg gcaagcgagc tgctgactcg ctttaaagcg catccggcag
    99481 tgcgcccgct tatcaaaaac acggaatcac tggagtatgg tgcgcatctg gtgccagaag
    99541 gtggcttgca cagtatgccg gtgcaatacg ccggtaacgg ctggctgctg gtgggcgatg
    99601 cgttgcgcag ttgcgtcaat accggaattt ccgtgcgcgg catggatatg gcgctgactg
    99661 gcgcgcaggc ggcggcacaa acgctgataa gcgcctgcca gcaccgcgag ccgcaaaatc
    99721 tgtttgcgct ttatcatcac aacgtagagc gcagcctgct atgggatgtt ctacaacgtt
    99781 atcagcatgt tccggcgctt ttgcaacgcc ctggctggta tcgggcgtgg cctgcgttaa
    99841 tgcaggatat ttcccgcgat ttatgggatc agggtgataa acctgttcca ccgctgcgcc
    99901 agttattctg gcgtcattta cgtcgtcatg gcctgtggca tctggcgggc gatgttatca
    99961 ggagtctgcg atgtctgtag cccataatct ctggcgcgtt gctgatgcgc cgcacattgt
   100021 tccggctgac tccgttgagc gtcagatggt acaacggttg attaacgcct gtccggcagg
   100081 tcttttttcg ctcacaccgg aaggtgactt acgcgttgat tatcacggct gcctggagtg
   100141 tggcacctgc cgtttgctgt gcgacgaatc cacactacaa cagtggcgct atccgccttc
   100201 cggattcggc atcacctacc gctttggata aataataagg ataatttatg cccctcttac
   100261 acctgctccg ccagaatccg gtgattgctg ccgttaaaga taatgccagc ctgcaactgg
   100321 caatcgactc tgaatgccag tttatttccg tgttgtacgg caatatctgc accatcagta
   100381 atatcgttaa aaagattaag aacgccggga aatatgcttt tattcatgtt gatttgctgg
   100441 aaggcgcgtc aaataaagaa gtcgtgattc agtttttgaa actggtgacc gaggcggacg
   100501 gcattatcag taccaaagcc tcaatgttga aagcggcaag agcggaaggt tttttctgta
   100561 ttcatcgcct gtttattgtt gattcaattt cgtttcacaa cattgataag caagttgcgc
   100621 aatcgaatcc ggattgtatt gagatcctgc caggctgtat gcccaaagtg ctgggctggg
   100681 tgacagagaa aatccgccaa ccgctgattg ccggtgggct ggtgtgcgat gaagaagatg
   100741 cgcgtaatgc gattaacgcg ggtgtcgtgg cgctttccac cacgaatacc ggggtctgga
   100801 cgttagcgaa aaaattactt tgacgggata atcgtaacca attgaatttg gtttgatttg
   100861 taggccgcac gccatatccg acattcagcg cctgatgcga cgcttgacgc gtcttatcag
   100921 gcctacaagt ccccaggcca gaaccgtagg caggatcatc cggcaacacg ttattggcaa
   100981 tctgcgtgaa tgtttgtcac cagtgcctcc agcaccactt tccagtcatc aaccacgcca
   101041 acatctgcct gtgaaaatac cgcagcgctg gcgtcatgat taatcgccac cacaaacttg
   101101 ctattgcgta cgcccgccat taacgctgct gcgccagaag cgccaacaac aatacacacc
   101161 tcaggtgcca gcagatgtcc ggaaatacca atcacttttt cagcatcaac gccaccgttc
   101221 atcactcgcg cccgactgta gcccacttcg gctcccagct tttctgccag catagcgatt
   101281 tcctggttat ctgcctctcc gccctgcccc accaccagca cccgtctggc ctcagctaat
   101341 gggtcgcgag taacattttt cagatcctct gtgctaacca gccagtcggg aagcgcaccc
   101401 gggacaatgt tcagttgttg cattccagag ggcaacgtgg cgtttttcgc tgctcccgcc
   101461 tgacgcgcca gggaaaggca tagcgggcgt ttttctgttt gcagcgtcgc cgttagcgca
   101521 tttccccagt gggatttgcg cacgcttacg gttggaatat ctaacgaagt cacctggcag
   101581 atgcttgcac catgtaaacg ccaggccagc cgggtcgaca gttcatcgcc aaacgttccc
   101641 ggcggaaaga gaacaacgtc cgcaggcgtt cgctgccact gttccaccaa cgcatccagc
   101701 acctgttccg ccactaccgg ctgaggttca atctgccagt gcgccagggt acaaccgctg
   101761 aaatcctgcg ccgccagcca gcttgctatt gcggcattct cctgatttat tgtcacaatt
   101821 gcgatattca tggctgcatc ctttggcgta aatagtcctg ccatagcttt tgagcttttt
   101881 ctgctaccgt ttgcccatca atcagcgtcg ctccgcgtcg ttgctctgct cttgccagtt
   101941 gcagacactg catcgctggc atttcggcag caacagtttt acggataatt tctgctttcc
   102001 ccgcagccat tcgctggcgc attcccggta ctggcaaggc cacttcaccg cactgacgca
   102061 cggcaatcac cgcaggcaag cgaacccggc aacaacgcaa cccatgttca gtacgctgtt
   102121 cgagggtgat aaacagcgcg tcgagagtga aacgctccac ctgggtgaag cagggccagc
   102181 ccagcatttc cgccagcaaa aatggcgttt gcccattctg cccttcgctg ctttggcagc
   102241 cggtaattat cagatccagc ggattctgat gctgccattc ggcaatatgg cgagcgacaa
   102301 attccggcgc aaagcgcaga tctgccgccg tctccagcaa tacagcttcc tcaaacccaa
   102361 gagccatgag atagcgcagc cagtgcaatg cccgttcatc ccccatgctc aacgcggtta
   102421 aagacatcgg cgtaccgttt tttctctgcg ccaacagcag cgcggcagcg gcctgttcat
   102481 cagcaccgag taaacttcgc agtagcgaaa tatccggtcc gcttttaccc tgagccgccg
   102541 cctgccactc tttttccgcc agcattccgg catccggttc ggctttaaac gctaacagaa
   102601 tgttcattcg cacctcctac atttcccgcc gccaccagcg ggagggcttt agtttctggt
   102661 gcccatagcc aggtcacaac aaaaccaacc aacaacactg tcgccaggag caataaggtg
   102721 acttgcattc cccactgcgc cagcacccac ggcagcaggc cagtacttac cgccgcgcca
   102781 agtcgactca tggcggtggc aaaaccgacg cccagcgagc gaatgtcagt aggaaaactt
   102841 tccgcaggca aaatgcccac cagattactg actgccgaaa tggtggtgct gaagagaaca
   102901 aaaagcagca gcgttaatga actgccggaa ggcaaacagg ccatgactac cagcgttgcc
   102961 gccagcagca aaaaacttcc cagcaaaaat ttgcgatgtg ccagcaggtg cgtcagaact
   103021 aatcccaaca gcgcgcccac aattaacaac gcattaagca tcaggctggc agtcagcgca
   103081 tcttccagac caatagtctg ggcgatagtt ggcagccagg tataaatcac aaaccatggg
   103141 attacgaggc agacaaagaa gacgctgtta aacgccgtgc gccgccagta acgcgaagag
   103201 aacaaggttt tgatgtgttt atgggtcgcc gttaccactt catcgcccag taaaacatgg
   103261 ggaccaaaat agcgatgcac gatagcgtga gcttctgcaa aacgcccctg gcgcagtagc
   103321 cagcgtggtg attctggcgt tccccagcgt aataacgtaa tcaacaacgc gggcagagct
   103381 gccgatgcca gtagccagcg ccaggcctcc gggttttcgg aaataaagtg atgtccggca
   103441 atacttgcca gcacatagcc tacggtccac accacgctga atgcgcccag caaaataccg
   103501 cgatggcggc gcggggaaaa ttcagccagc aaggtgtgac ctactgaata atcgcctccc
   103561 agactaatgc caatcaaaat gcgcagtcca ataagatgct ctggcgtggt ggcaaaaaat
   103621 tgtaaaaacg aagcaagcgt aatcagcaaa aagctgaagg tgaagatttt ttgccgacca
   103681 atatggtcgg agatccaccc aagaaccagg ctgccaagga acaaaccaag gagcgccgag
   103741 ccgccgatca ttcccgccat aaacggcgtc agttgcatgg cgggcgtaag ctgaataatg
   103801 gcgtaaccaa tgacgccgag aacataaccg tcggtcaggt gtgcgccgaa agtgagcgca
   103861 gcaatgcggc agtgaaaacg gttaagcggt aaatcatcca ttcgcaccgg tgaagtgttc
   103921 atctttaccc cttcattgtc cggtaataag aacggcttct cgcctgagaa gccgaaaagg
   103981 caacgccccg tcatcgcgga acgctgcctg acacattatt tttcaatcgg atagatagta
   104041 cccgtattca taatgccatt aggatcgaac tgctttttca gcccttccag caacgcccac
   104101 gcgctgccgt gttccagttt gctccagtga acgcgatgtt taccgatacc gtggtggtgc
   104161 accatcgaac cgccgaggcg aatggtttct tcacagatga tcttgttgag cggattgtgg
   104221 tacttgtcga tttcctcttc cggcttacag tcaacgacgt tgtaatcgta gacgaagtac
   104281 atgttggtgc cgttctgata gctatgagag gaatgaccgc ccagcatggt gatgtcgtcg
   104341 gcgtgcggga actcagtacg aatacggtta ataacgcttt cgtagatttc gtggatgcag
   104401 ctccagcagc cggacacttc agtggtaaag cccatgttgc cggttttgag gatctgcaca
   104461 cgttcggcag ccactttatc cggtccccag ttcaggttgt taaaccaggt ttcgatcagc
   104521 ttgctgtcca cgcgctggca ttgcgggtag cgggcaacga tttccgcaat cccttcgccc
   104581 gtcgccttcg caatgcgagg gttaccttca gccataaaga tcagcacgca ttttccgtct
   104641 gcaaaatggg tgaagtgttg ggtgccatct tcagcgtcat acaaacgagc gatcgacgga
   104701 cgataccctt ccaccatgac ttcacgcagg atgttgaagc cggttttcat gtcttccagg
   104761 atatagccgt agaagaggtt gttttccggg gtgaatttaa agattttcac tgttacttca
   104821 gtgatatagc aaagtgcacc ttcgttaccg atgatgatgt ggcgaatgtc cgggccagcc
   104881 gcgcggcgtg gcacgttttt aatgcgtgtg acggtgccat cagccaatac tgcttccaga
   104941 ccaacgacca tatcttcgat tgcgccgtag agtgtggaga actgcccgat actgcgggtt
   105001 gctaccaggc cgcccatctg agccagcggc tttgactgcg gagaatgccc cgtggtgtaa
   105061 cctttttcac gcaacgcatt ttccagcacc tccagcggaa cgccacattg cgccgtcgcc
   105121 tgcatattct caatatcaat attaatgatt tgattcatgg cggagccgtc gagcaccacc
   105181 gagttttcca caacggtttc cagcccacct tcagtggcgg aagcgccggt acgcggcaca
   105241 ccgttaattt tgtgcgcatt cataaaattc agcacacggg agacttgttc tgtggaaccg
   105301 agttttacga ccgctgccgg aatcggcaaa gtataaatac catgaatatc cggaaattta
   105361 cgaaaacggt caatactgtt cttctttaat actgtttcat cggtaattac gcgatctgca
   105421 ccaacaattt cctttagctg gtcgacaatc gctgcgcgag ataaagacat agtaattcct
   105481 tcctgataat aaaaatggaa taatcattaa taagagaaaa cagaaaccac cgttatggat
   105541 tagcgcacta aataaccacc atcgaccact aataaatgcc cattgacata atttgaagcc
   105601 ggacttgcga ggaatacagc tgcgcccatt aaatcctgag tatcgcccca acggtttgcc
   105661 ggaatatgat caagaacgcg ctgattggtt tctggattac tgcgtgtcgc cagcgtaata
   105721 tctgttgcat aatagccagg ggcgatacca tttacctgaa tattatattg acctagctca
   105781 tcacaataag ctttggtgaa cccggcaaga gcatgtttag tggcagaata tgcaggtgac
   105841 cattgtccac ctaagtaaga gaacaatgaa cagatattaa taattttgcc gcttttctgc
   105901 gggatcataa tttttgcagc ttcatagctt aactcgaatg cggcggtcag gttcacatca
   105961 atcatcggat cccagtcggc acgaccgaag tccagcacct tattcagctt acaaataccg
   106021 gcattgttta ccagaatatc aactgtaccg aaacgctcac agcaagcagc gataatcttc
   106081 tgcggcgcgc cttctgcggt gatatccacc tgcatgaagt ccacctcaac accctgtttt
   106141 tcaatcattt cctttgtttc gccgttatct ttgacgaaac taggaataaa gatatttgcg
   106201 ccagctttgg ccaacgccat ggcaaatgcc tggcctaaac cgctattccc accggtaaca
   106261 attgcggttt tacctttcag tgagaaaaaa tccattgaga acgcattgag agattcgatt
   106321 gacatagtta gctccaattt ccgtaaggca aaaaaaaaga aaggtaactt cccccgcgag
   106381 agggaagtta cctttctcat tatctctggt aactggagat aatttttagc ataacggcat
   106441 aactgctaat gtgatcgtaa tcacagtgtg atattcgtat tgtcaaatag ttttaaaaag
   106501 cagaagcgtg attactctca cacaacacct ctgctttcca tgttatttct tattgcaatt
   106561 actagagacc tgagaaaagt aatccacctg aaagggcggc ttacttttct cttttttttg
   106621 tctattacaa atgggaatac gatatgcaac acaactcata tcgccgttgg ataaccctcg
   106681 cgataattag ttttagcggc ggcgttagtt tcgacctggc ttatttacgt tatatttatc
   106741 aaattcccat ggcgaaattt atgggattca gcaataccga gataggttta ataatgagta
   106801 cctttggtat tgcggctatt attctttatg cccccagcgg cgttattgcc gataaatttt
   106861 cacaccgcaa aatgattact tccgcgatga tcattaccgg attactgggt ctgttaatgg
   106921 caacgtatcc accgctgtgg gtaatgctct gtattcaggt cgcctttgcg ataacgacga
   106981 ttttaatgct gtggtcggtg tcgattaaag ccgcctcgtt gctgggcgat catagtgagc
   107041 aagggaaaat tatgggctgg atggaagggc tgcgcggcgt cggtgtaatg tcgctggcgg
   107101 tgtttaccat gtgggtcttt tctcgctttg caccggatga cagcgccagc ctgaaaacag
   107161 tcattatcat ctacagtgtg gtttacatct tgttggggat tctgtgctgg ttttttgtta
   107221 gcgataacaa caacctgcgc agtgccaata acgaagaaaa acagtcattc cagcttagcg
   107281 acatccttgc cgttttgcgt atcagcacca cctggtattg cagcatggtg atttttggtg
   107341 tcttcacaat ctacgccatt ctgagttact ccaccaacta tctgaccgaa atgtacggca
   107401 tgtcgctggt ggcagcgagc tacatgggga ttgtgatcaa caaaatcttc cgcgcgctgt
   107461 gcggcccact tggcggcatt atcaccacct acagtaaagt gaaatcccct acccgcgtga
   107521 tccaaatcct ttccgtactc ggcctgctgg cgttaactgc cctgctcgtc acgaactcta
   107581 acccgcaatc ggtcgcgatg gggattggcc tgattttact gctgggattc acctgttacg
   107641 cctcacgcgg gctgtactgg gcctgccctg gcgaagcgag aacaccgtct tacattatgg
   107701 gcaccacggt aggtatttgt tcggtgattg gattcctgcc ggatgtcttc gtttacccga
   107761 ttatcggcca ctggcaagac accctgcccg cagcagaagc ctaccgcaat atgtggctga
   107821 tgggaatggc ggcgcttgcc atggtgattg tctttacctt tttgctgttc caaaaaattc
   107881 gtactgctga tagcgccccc gcaatggcta gcagcaagta agccaggaaa attacaggag
   107941 aaaaatcatg tcgaaaaaat acatcatagg gattgatggc ggaagtcaga gcacaaaagt
   108001 ggtgatgtac gatctggaag gtaacgtggt ttgcgaaggc aaaggcttat tacagccgat
   108061 gcacacgcca gatgccgata ctgcagaaca tcctgacgac gatttatggg catcattatg
   108121 ttttgccggt cacgatttga tgagtcagtt tgctgggaat aaagaagata ttgtcggtat
   108181 tggtctggga tccatccgtt gctgtcgtgc gttattgaaa gccgatggca cgcctgctgc
   108241 gccgttgatt agctggcagg atgcacgcgt tacacgccct tacgaacaca ctaatcctga
   108301 cgtggcatat gtcacctctt tttcgggtta tctgacgcat cgcttaaccg gcgagtttaa
   108361 agacaatatc gccaactatt ttggtcagtg gccggtggat tataagagct ggacatggag
   108421 cgaagatgct gcggtaatgg ataagtttaa tatcccccgt catatgctgt ttgatgtgca
   108481 aatgcctggc accgtcctcg gacatatcac accacaagcc gcactggcga cacatttccc
   108541 ggcaggactg ccggttgttt gtaccaccag tgataaaccg gtagaagctc tgggggccgg
   108601 attactggat gatgaaactg cggtaatttc tcttggcact tacatcgcat tgatgatgaa
   108661 cggcaaagca ctgccgaaag atccggtggc gtactggccg attatgtctt ctattccgca
   108721 aacattgctg tatgaaggtt acggtattcg caaaggtatg tggacggtga gctggctgcg
   108781 tgacatgtta ggcgagtcgt taattcagga tgccagggcg caggatcttt caccggaaga
   108841 tttactcaac aagaaagcct ctagtgtgcc acctggctgt aatgggctga tgacggtgct
   108901 ggactggctg accaatccgt gggaaccgta caaacgcggg attatgatcg gctttgattc
   108961 cagcatggat tacgcatgga tatatcgttc gatattggaa agcgtggcgc taacgctgaa
   109021 gaacaattac gacaatatgt gtaatgaaat gaatcacttt gcgaagcatg tgatcattac
   109081 tggcggcggt tcgaacagcg atctgtttat gcaaattttt gccgacgtgt tcaaccttcc
   109141 ggcacgccgt aacgccatta acggttgtgc aagtctggga gcagcgatta atacagcggt
   109201 aggtctgggg ctatacccgg attacgcaac ggctgttgat aagatggttc gcgtgaaaga
   109261 tatctttata ccgattgaga gcaatgccaa acgctacgac gcgatgaata aaggcatttt
   109321 caaagaccta accaaacata ctgatgtgat cctgaaaaaa tcgtatgaag tgatgcatgg
   109381 ggaattgggg aatgtggatt cgatccagag ctggtcgaat gcgtaagtga ggaaggccgg
   109441 gcgggaaact gcccggcctg aacatacctg aatggttatc cccgctgacg cggggaacat
   109501 aagttcgaca acttaaaaaa tgataacaag tccggtttat ccccgctgat gcagggaaca
   109561 taataaaaac tttaactagt tcggtttatc cctgttttta caaggaatat taactacact
   109621 cgatgtcttt agaataaaag acaatacgaa tcgcacttta tcaattttca cttaaagtat
   109681 gaactggata tctttattca taagaactat tcatcaacag cgtatagagg cggttatgta
   109741 aaaccactca ttagcctcaa aacaaagaca aacttccaga tttattactt aaccaatgaa
   109801 taacaagctt aaaaaacata ctgtta
//
LOCUS       DAZFXT010000002        89692 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.002, whole genome shotgun sequence.
ACCESSION   DAZFXT010000002 DAZFXT010000000
VERSION     DAZFXT010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 89692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 89692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..89692
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.002"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..167
                     /gene="yhfL"
                     /locus_tag="ACHKW0_000114"
     CDS             <1..167
                     /gene="yhfL"
                     /locus_tag="ACHKW0_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HFO7473011.1"
                     /translation="NKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISK
                     IIGGCGPTAQ"
     gene            462..1799
                     /gene="frlA"
                     /locus_tag="ACHKW0_000115"
     CDS             462..1799
                     /gene="frlA"
                     /locus_tag="ACHKW0_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HFO7473012.1"
                     /translation="MGSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWL
                     TVLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDA
                     PSLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAK
                     IIPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMT
                     GEIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPAL
                     GSTAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSI
                     ILQGAQGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGL
                     MTTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
     gene            1820..2842
                     /gene="frlB"
                     /locus_tag="ACHKW0_000116"
     CDS             1820..2842
                     /gene="frlB"
                     /locus_tag="ACHKW0_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HFO7473013.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHTIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQHGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            2892..3722
                     /gene="frlC"
                     /locus_tag="ACHKW0_000117"
     CDS             2892..3722
                     /gene="frlC"
                     /locus_tag="ACHKW0_000117"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HFO7473014.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAMDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYDGYCTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            3719..4504
                     /gene="frlD"
                     /locus_tag="ACHKW0_000118"
     CDS             3719..4504
                     /gene="frlD"
                     /locus_tag="ACHKW0_000118"
                     /EC_number="2.7.1.218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HFO7473015.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGPI
                     TWVGDDDYGTKLKQDLARMDVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            4604..5335
                     /gene="frlR"
                     /locus_tag="ACHKW0_000119"
                     /gene_synonym="yhfR"
     CDS             4604..5335
                     /gene="frlR"
                     /locus_tag="ACHKW0_000119"
                     /gene_synonym="yhfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator FrlR"
                     /protein_id="HFO7473016.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(5487..6572)
                     /gene="yhfS"
                     /locus_tag="ACHKW0_000120"
     CDS             complement(5487..6572)
                     /gene="yhfS"
                     /locus_tag="ACHKW0_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7473017.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANISTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(6584..7888)
                     /gene="yhfT"
                     /locus_tag="ACHKW0_000121"
     CDS             complement(6584..7888)
                     /gene="yhfT"
                     /locus_tag="ACHKW0_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HFO7473018.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVKQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(7900..8253)
                     /gene="yhfU"
                     /locus_tag="ACHKW0_000122"
     CDS             complement(7900..8253)
                     /gene="yhfU"
                     /locus_tag="ACHKW0_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HFO7473019.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(8264..9142)
                     /gene="php"
                     /locus_tag="ACHKW0_000123"
     CDS             complement(8264..9142)
                     /gene="php"
                     /locus_tag="ACHKW0_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HFO7473020.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVATRSVQELA
                     QEMVDEIEQGIDGTDLKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            complement(9139..10365)
                     /gene="yhfW"
                     /locus_tag="ACHKW0_000124"
     CDS             complement(9139..10365)
                     /gene="yhfW"
                     /locus_tag="ACHKW0_000124"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7473021.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(10365..11528)
                     /gene="yhfX"
                     /locus_tag="ACHKW0_000125"
     CDS             complement(10365..11528)
                     /gene="yhfX"
                     /locus_tag="ACHKW0_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HFO7473022.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRIQSVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIVIEQLNAPSATSCTSLPLLAQYGVTHAEPGHALTGTIPANQQGDQSERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     SLAGEFPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPKIVGRYDSLGNSLGA"
     gene            complement(11612..11974)
                     /gene="yhfY"
                     /locus_tag="ACHKW0_000126"
     CDS             complement(11612..11974)
                     /gene="yhfY"
                     /locus_tag="ACHKW0_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HFO7473023.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AITHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            complement(11991..12896)
                     /gene="yhfZ"
                     /locus_tag="ACHKW0_000127"
     CDS             complement(11991..12896)
                     /gene="yhfZ"
                     /locus_tag="ACHKW0_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator YhfZ"
                     /protein_id="HFO7473024.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(13165..14169)
                     /gene="trpS"
                     /locus_tag="ACHKW0_000128"
     CDS             complement(13165..14169)
                     /gene="trpS"
                     /locus_tag="ACHKW0_000128"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HFO7473025.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(14162..14920)
                     /gene="gph"
                     /locus_tag="ACHKW0_000129"
     CDS             complement(14162..14920)
                     /gene="gph"
                     /locus_tag="ACHKW0_000129"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HFO7473026.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(14913..15590)
                     /gene="rpe"
                     /locus_tag="ACHKW0_000130"
     CDS             complement(14913..15590)
                     /gene="rpe"
                     /locus_tag="ACHKW0_000130"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HFO7473027.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(15608..16444)
                     /gene="dam"
                     /locus_tag="ACHKW0_000131"
     CDS             complement(15608..16444)
                     /gene="dam"
                     /locus_tag="ACHKW0_000131"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7473028.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(16551..17837)
                     /gene="damX"
                     /locus_tag="ACHKW0_000132"
     CDS             complement(16551..17837)
                     /gene="damX"
                     /locus_tag="ACHKW0_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HFO7473029.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRETAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPAATSTPATKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYGNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(17929..19017)
                     /gene="aroB"
                     /locus_tag="ACHKW0_000133"
     CDS             complement(17929..19017)
                     /gene="aroB"
                     /locus_tag="ACHKW0_000133"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HFO7473030.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(19074..19595)
                     /gene="aroK"
                     /locus_tag="ACHKW0_000134"
     CDS             complement(19074..19595)
                     /gene="aroK"
                     /locus_tag="ACHKW0_000134"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HFO7473031.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(19996..21234)
                     /gene="hofQ"
                     /locus_tag="ACHKW0_000135"
     CDS             complement(19996..21234)
                     /gene="hofQ"
                     /locus_tag="ACHKW0_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HFO7473032.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEDNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(21146..21550)
                     /gene="hofP"
                     /locus_tag="ACHKW0_000136"
     CDS             complement(21146..21550)
                     /gene="hofP"
                     /locus_tag="ACHKW0_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HFO7473033.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            complement(21540..21980)
                     /gene="hofO"
                     /locus_tag="ACHKW0_000137"
     CDS             complement(21540..21980)
                     /gene="hofO"
                     /locus_tag="ACHKW0_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HFO7473034.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            complement(21964..22503)
                     /gene="hofN"
                     /locus_tag="ACHKW0_000138"
     CDS             complement(21964..22503)
                     /gene="hofN"
                     /locus_tag="ACHKW0_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HFO7473035.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTS
                     SAEARVDAVLLQGEQQLAHSLQITKPRLLERQQIREQRLQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(22503..23282)
                     /gene="hofM"
                     /locus_tag="ACHKW0_000139"
     CDS             complement(22503..23282)
                     /gene="hofM"
                     /locus_tag="ACHKW0_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HFO7473036.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            23402..25954
                     /gene="mrcA"
                     /locus_tag="ACHKW0_000140"
     CDS             23402..25954
                     /gene="mrcA"
                     /locus_tag="ACHKW0_000140"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HFO7473037.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(26119..26679)
                     /gene="nudE"
                     /locus_tag="ACHKW0_000141"
     CDS             complement(26119..26679)
                     /gene="nudE"
                     /locus_tag="ACHKW0_000141"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HFO7473038.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            26999..29134
                     /gene="igaA"
                     /locus_tag="ACHKW0_000142"
     CDS             26999..29134
                     /gene="igaA"
                     /locus_tag="ACHKW0_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HFO7473039.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            29199..29867
                     /gene="yrfG"
                     /locus_tag="ACHKW0_000143"
     CDS             29199..29867
                     /gene="yrfG"
                     /locus_tag="ACHKW0_000143"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HFO7473040.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            29878..30279
                     /gene="hslR"
                     /locus_tag="ACHKW0_000144"
     CDS             29878..30279
                     /gene="hslR"
                     /locus_tag="ACHKW0_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HFO7473041.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            30304..31182
                     /gene="hslO"
                     /locus_tag="ACHKW0_000145"
     CDS             30304..31182
                     /gene="hslO"
                     /locus_tag="ACHKW0_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HFO7473042.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(31317..33041)
                     /gene="yhgE"
                     /locus_tag="ACHKW0_000146"
     CDS             complement(31317..33041)
                     /gene="yhgE"
                     /locus_tag="ACHKW0_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HFO7473043.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQAERILFAF
                     NDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKAWRDLTVDGE
                     TLEINLSK"
     gene            33419..35041
                     /gene="pckA"
                     /locus_tag="ACHKW0_000147"
     CDS             33419..35041
                     /gene="pckA"
                     /locus_tag="ACHKW0_000147"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HFO7473044.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(35117..36469)
                     /gene="envZ"
                     /locus_tag="ACHKW0_000148"
     CDS             complement(35117..36469)
                     /gene="envZ"
                     /locus_tag="ACHKW0_000148"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HFO7473045.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(36466..37185)
                     /gene="ompR"
                     /locus_tag="ACHKW0_000149"
     CDS             complement(36466..37185)
                     /gene="ompR"
                     /locus_tag="ACHKW0_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HFO7473046.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            37413..37889
                     /gene="greB"
                     /locus_tag="ACHKW0_000150"
     CDS             37413..37889
                     /gene="greB"
                     /locus_tag="ACHKW0_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HFO7473047.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            37986..40307
                     /gene="yhgF"
                     /locus_tag="ACHKW0_000151"
     CDS             37986..40307
                     /gene="yhgF"
                     /locus_tag="ACHKW0_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HFO7473048.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            40745..40972
                     /gene="feoA"
                     /locus_tag="ACHKW0_000152"
     CDS             40745..40972
                     /gene="feoA"
                     /locus_tag="ACHKW0_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HFO7473049.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            40989..43310
                     /gene="feoB"
                     /locus_tag="ACHKW0_000153"
     CDS             40989..43310
                     /gene="feoB"
                     /locus_tag="ACHKW0_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HFO7473050.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            43310..43546
                     /gene="feoC"
                     /locus_tag="ACHKW0_000154"
     CDS             43310..43546
                     /gene="feoC"
                     /locus_tag="ACHKW0_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HFO7473051.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            43749..44627
                     /gene="rpnA"
                     /locus_tag="ACHKW0_000155"
     CDS             43749..44627
                     /gene="rpnA"
                     /locus_tag="ACHKW0_000155"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HFO7473052.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGQVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLTDI
                     MRFTGVSEEELAAASQ"
     gene            complement(44656..45426)
                     /gene="bioH"
                     /locus_tag="ACHKW0_000156"
     CDS             complement(44656..45426)
                     /gene="bioH"
                     /locus_tag="ACHKW0_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HFO7473053.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            45464..46147
                     /gene="gntX"
                     /locus_tag="ACHKW0_000157"
     CDS             45464..46147
                     /gene="gntX"
                     /locus_tag="ACHKW0_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HFO7473054.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRAARTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCRWDSKAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            46206..46781
                     /gene="nfuA"
                     /locus_tag="ACHKW0_000158"
     CDS             46206..46781
                     /gene="nfuA"
                     /locus_tag="ACHKW0_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HFO7473055.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            47142..48458
                     /gene="gntT"
                     /locus_tag="ACHKW0_000159"
     CDS             47142..48458
                     /gene="gntT"
                     /locus_tag="ACHKW0_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7473056.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(48503..50587)
                     /gene="malQ"
                     /locus_tag="ACHKW0_000160"
     CDS             complement(48503..50587)
                     /gene="malQ"
                     /locus_tag="ACHKW0_000160"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HFO7473057.1"
                     /translation="MESKRLDNAALAAGISPNYINAYGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDHE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(50597..52990)
                     /gene="malP"
                     /locus_tag="ACHKW0_000161"
     CDS             complement(50597..52990)
                     /gene="malP"
                     /locus_tag="ACHKW0_000161"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HFO7473058.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            53602..56307
                     /gene="malT"
                     /locus_tag="ACHKW0_000162"
     CDS             53602..56307
                     /gene="malT"
                     /locus_tag="ACHKW0_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HFO7473059.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(56350..57366)
                     /gene="rtcA"
                     /locus_tag="ACHKW0_000163"
     CDS             complement(56350..57366)
                     /gene="rtcA"
                     /locus_tag="ACHKW0_000163"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003963 - RNA-3'-phosphate cyclase
                     activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HFO7473060.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGIVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(57370..58596)
                     /gene="rtcB"
                     /locus_tag="ACHKW0_000164"
     CDS             complement(57370..58596)
                     /gene="rtcB"
                     /locus_tag="ACHKW0_000164"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HFO7473061.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IEMAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQETLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            58785..60383
                     /gene="rtcR"
                     /locus_tag="ACHKW0_000165"
     CDS             58785..60383
                     /gene="rtcR"
                     /locus_tag="ACHKW0_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HFO7473062.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDTRSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSVFQLISGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(60365..61123)
                     /locus_tag="ACHKW0_000166"
     CDS             complement(60365..61123)
                     /locus_tag="ACHKW0_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HFO7473063.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(61140..61970)
                     /gene="glpG"
                     /locus_tag="ACHKW0_000167"
     CDS             complement(61140..61970)
                     /gene="glpG"
                     /locus_tag="ACHKW0_000167"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HFO7473064.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(62015..62341)
                     /gene="glpE"
                     /locus_tag="ACHKW0_000168"
     CDS             complement(62015..62341)
                     /gene="glpE"
                     /locus_tag="ACHKW0_000168"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HFO7473065.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            62531..64036
                     /gene="glpD"
                     /locus_tag="ACHKW0_000169"
     CDS             62531..64036
                     /gene="glpD"
                     /locus_tag="ACHKW0_000169"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HFO7473066.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(64088..64693)
                     /locus_tag="ACHKW0_000170"
     CDS             complement(64088..64693)
                     /locus_tag="ACHKW0_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473067.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(64696..65463)
                     /locus_tag="ACHKW0_000171"
     CDS             complement(64696..65463)
                     /locus_tag="ACHKW0_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473068.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDETVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(65466..66176)
                     /locus_tag="ACHKW0_000172"
     CDS             complement(65466..66176)
                     /locus_tag="ACHKW0_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473069.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(66192..67700)
                     /locus_tag="ACHKW0_000173"
     CDS             complement(66192..67700)
                     /locus_tag="ACHKW0_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7473070.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFHQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNADNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNDDKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
     gene            complement(67825..70272)
                     /gene="glgP"
                     /locus_tag="ACHKW0_000174"
     CDS             complement(67825..70272)
                     /gene="glgP"
                     /locus_tag="ACHKW0_000174"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HFO7473071.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(70291..71724)
                     /gene="glgA"
                     /locus_tag="ACHKW0_000175"
     CDS             complement(70291..71724)
                     /gene="glgA"
                     /locus_tag="ACHKW0_000175"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HFO7473072.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(71724..73019)
                     /gene="glgC"
                     /locus_tag="ACHKW0_000176"
     CDS             complement(71724..73019)
                     /gene="glgC"
                     /locus_tag="ACHKW0_000176"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HFO7473073.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(73037..75010)
                     /gene="glgX"
                     /locus_tag="ACHKW0_000177"
     CDS             complement(73037..75010)
                     /gene="glgX"
                     /locus_tag="ACHKW0_000177"
                     /EC_number="3.2.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HFO7473074.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(75007..77193)
                     /gene="glgB"
                     /locus_tag="ACHKW0_000178"
     CDS             complement(75007..77193)
                     /gene="glgB"
                     /locus_tag="ACHKW0_000178"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HFO7473075.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(77466..78569)
                     /gene="asd"
                     /locus_tag="ACHKW0_000179"
     CDS             complement(77466..78569)
                     /gene="asd"
                     /locus_tag="ACHKW0_000179"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7473076.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            78762..79355
                     /gene="yhgN"
                     /locus_tag="ACHKW0_000180"
     CDS             78762..79355
                     /gene="yhgN"
                     /locus_tag="ACHKW0_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HFO7473077.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(79401..80696)
                     /locus_tag="ACHKW0_000181"
     CDS             complement(79401..80696)
                     /locus_tag="ACHKW0_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO7473078.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEHKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWIITRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAIEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVPIR
                     LSDVPGLMYDRALRNAQPELFSLHENDANYAVMPAHTPVDFYQLFVAELAIFAKESIS
                     IERLSSCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(80603..82699)
                     /locus_tag="ACHKW0_000182"
     CDS             complement(80603..82699)
                     /locus_tag="ACHKW0_000182"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HFO7473079.1"
                     /translation="MEKHAAELLLQRMLGNTTATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLQGEVDCLLVSPERLANQDFIETVLYPIADRIGLLVVDEAHCISDWGHDF
                     RPDYRRILDILRQLPANTPVLGTTATANNRVVEDIRQQLGDIVIQRGTLARESLALDA
                     LVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDADLVAEWLRTNGISAFAYYSGVTCE
                     GAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFVIHYQMPGSIVGYYQQV
                     GRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEILNVLSENDGLTLRGIEQ
                     RTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDHERINHLMNQRKNELAD
                     VESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPDIDSGLLHAANLFIKHA
                     DLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDSGWGKQVAQEKKTGRFS
                     DELVEACAEMVRQRWNPHPEPTWVCCVPSLRHLDLVPDFARRLAAKLGLPFIDAIEKV
                     VDNPPQKMQQNRFHQCQNLDGAFVITPPLMPGPALLVDDIVDSAWTLTVLTALLRQAG
                     CPTVYPLALASTSVKN"
     gene            complement(82839..84179)
                     /gene="gntU"
                     /locus_tag="ACHKW0_000183"
     CDS             complement(82839..84179)
                     /gene="gntU"
                     /locus_tag="ACHKW0_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015128 - gluconate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035429 - gluconate transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HFO7473080.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPIIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(84183..84710)
                     /gene="gntK"
                     /locus_tag="ACHKW0_000184"
     CDS             complement(84183..84710)
                     /gene="gntK"
                     /locus_tag="ACHKW0_000184"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HFO7473081.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(84849..85844)
                     /gene="gntR"
                     /locus_tag="ACHKW0_000185"
     CDS             complement(84849..85844)
                     /gene="gntR"
                     /locus_tag="ACHKW0_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HFO7473082.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(86068..86763)
                     /gene="yhhW"
                     /locus_tag="ACHKW0_000186"
     CDS             complement(86068..86763)
                     /gene="yhhW"
                     /locus_tag="ACHKW0_000186"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HFO7473083.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(86886..87923)
                     /gene="yhhX"
                     /locus_tag="ACHKW0_000187"
     CDS             complement(86886..87923)
                     /gene="yhhX"
                     /locus_tag="ACHKW0_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7473084.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            88255..88743
                     /gene="yhhY"
                     /locus_tag="ACHKW0_000188"
     CDS             88255..88743
                     /gene="yhhY"
                     /locus_tag="ACHKW0_000188"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7473085.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            88980..>89692
                     /gene="tssD"
                     /locus_tag="ACHKW0_000189"
     CDS             88980..>89692
                     /gene="tssD"
                     /locus_tag="ACHKW0_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein TssD"
                     /protein_id="HFO7473086.1"
                     /translation="MSNIVYLTVTGEQQGSISAGCGTSESTGNRWQSGHEDEIFTFSL
                     LNNINNTGLGSQFHGITFCKLIDKSTPLFINSINNNEQLFMGFDFYRINRFGRWEKYY
                     YIQLRGAFLSAIHHQIIENQLDTETITISYEFILCQHLIANTEFSYLALPENYNRLFL
                     PNSKNQTNNRFKTLNSKAIGRLLAAGGVYNGNIEGFRDTAEKLGGDAIKGYDQILNEK
                     TAGIAIATASILLTKRSNV"
BASE COUNT        21762 a        24379 c        22410 g        21141 t
ORIGIN      
        1 tgaacaaatt tattaaagtt gcactggtag gtgcagtact ggctacgtta actgcatgta
       61 ctggtcatat tgaaaaccgt gataagaact gctcttacga ctacctgctg cacccggcaa
      121 tttctatttc taaaatcatt ggcggttgcg gtcctactgc acagtaagtc tctgacagaa
      181 atcggctaac accggtttaa ttgcccgacg ccctctggat tttccgaggg cgtatttgtt
      241 tctatatcct tcttgccccc gcccgtaaaa atattttcac tttaaattca atttgataac
      301 tacatccatt agccacagcg cgatcctgtg cgaaattttg tgatcttcct ccacattaca
      361 taacatcata tgttgttata ttcatcatgc attgtcatgt taccttttaa atgactgcaa
      421 actctcccct acaacgtatt ggacaagaaa aaggtatcgc tatgggaagc caggaactcc
      481 aacgcaagct cggattttgg gccgttcttg caatcgccgt cgggacaacc gtcggctccg
      541 gtatttttgt atctgtgggt gaagtggcaa aagcagcggg cacgccgtgg cttacggtgc
      601 tcgcgtttgt cattggcggg ttaattgtga tcccgcaaat gtgcgtctat gcggaactat
      661 ccaccgctta tccggaaaat ggcgcagatt atgtttatct gaaaaatgcc ggaagccgac
      721 cgctggcttt cctctccggc tgggccagct tctgggccaa cgatgcgccg tcattgtcga
      781 ttatggcgct ggcgattgtc agcaatcttg gctttttaac gcctatcgat ccgttgctcg
      841 gtaaatttat cgccgccgga ttaattatcg cctttatgtt gctacacctg cgctccgttg
      901 aaggcggcgc agcgtttcag acgctaatta ccatcgccaa aattatcccg ttcactatcg
      961 tcattggcct tgggatcttc tggtttaaag cggagaattt tgccgcccct accaccactg
     1021 cgattggcgc aacgggcagc tttatggcgc tgctggcggg gatctctgcc accagttggt
     1081 cgtataccgg catggcctct atctgttata tgaccggcga aattaaaaac cccggaaaaa
     1141 ccatgccacg agcgctgatt ggttcctgtc tgctggttct ggtgctctac accctgctgg
     1201 cgctggtgat ttccggcctg atgcccttcg acaaactcgc caattctgaa acgccgattt
     1261 ccgacgccct gacctggatc cccgcactcg gcagcaccgc tgggatcttt gttgccatca
     1321 cggcgatgat cgtcattctt ggttcgcttt ccagctgcgt gatgtaccag ccgcggctgg
     1381 aatacgcgat ggcgaaagac aacctgttct ttaaatgctt cggccatgtg catccgaaat
     1441 acaacacgcc ggatgtctcc atcatcctgc aaggggcgca ggggatcttc ttcatcttcg
     1501 tttccgatct caccagcctg ctgggttatt tcaccctggt gatgtgtttc aaaaataccc
     1561 tcaccttcgg ctccatcatc tggtgtcgta aacgcgacga ttacaaaccg ctgtggcgta
     1621 ctccggcttt cgggctgatg accaccctcg ccattgcgtc aagcctcatt ctggtcgcct
     1681 caacctttgt ctgggcaccg attcccggcc ttatctgcgc cgtcatcgtt attgctactg
     1741 gtctgcctgc ttacgccttc tgggcgaagc gtagccgcca gctcaacgct ttgtcgtaat
     1801 gttatctgga gaaaataaaa tgttggatat tgataaaagc accgtggact ttctggtcac
     1861 cgaaaatatg gttcaggaag tggaaaaagt tctcagccat gacgttccgc tggtgcacac
     1921 catcgtggaa gagatggtga agcgcgacat tgatcgtatt tatttcgttg cctgcggatc
     1981 gccactcaac gcggcgcaaa cggcgaaaca tctggcggat cgcttttccg atcttcaggt
     2041 ctacgccatt tccggctggg agttctgcga taacaccccg tatcgcctcg acgatcgttg
     2101 cgcggtgatt ggcgtttctg actacggtaa aacggaagag gtaatcaaag cgctggagct
     2161 gggccgggcc tgcggcgcac tcactgcggc gttcaccaaa cgcgcggaca gcccgattac
     2221 ctcggcggcg gaatttagca ttgattatca ggccgactgt atctgggaaa ttcacctgct
     2281 gctctgctac agcgtggtgc tggagatgat cacccgcctc gcgccgaacg cggaaatcgg
     2341 caagatcaaa aacgatctca agcagttgcc gaatgcgctc ggtcatctgg tacgcacctg
     2401 ggaagaaaaa ggccgccagc atggtgaact ggccagccag tggccgatga tttataccgt
     2461 tgctgcgggt ccgctgcgtc cgctgggtta caaagaaggc attgtaacgc tgatggaatt
     2521 tacctggacg cacggctgcg tgattgagag cggagagttc cgccatggcc cgctggagat
     2581 tgtcgaaccg ggcgttccgt tcctgttcct gctcggcaat gatgaaagtc gccacaccac
     2641 cgaacgcgcc attaactttg ttaaacagcg tactgacaac gtgatcgtca tcgattacgc
     2701 cgaaatttcg caagggctgc acccgtggct ggcaccgttc ctgatgttcg tgccaatgga
     2761 gtggctctgc tactacctgt ctatttacaa agatcacaac ccggatgaac gccgctatta
     2821 cggtggtctg gtggaatatt aatccctctc ccggcccggc aacgggccga atttacgcaa
     2881 ggagttaccc gatgaaaaca ggtatgttta cctgcggcca ccagcggctg cctattgaac
     2941 acgcatttcg tgatgcaagc gagctgggtt acgacggcat cgaaatttgg ggcggtcgcc
     3001 cgcacgcgtt cgcgccggac ttaaaagcgg gcggcatcaa acaaatcaag gcgctggcgc
     3061 agacgtatca gatgccgatt atcggctata cgccagaaac caacggctat ccgtataaca
     3121 tgatgctggg cgatgaacat atgcgtcgcg aaagcctcga catgatcaag ctggcgatgg
     3181 atatggcaaa agagatgaac gcgggttata cgctgatttc cgcggcccac gcgggctatc
     3241 tcacgccacc taatgttatc tggggacggc tggcagagaa cctaagcgaa ctgtgtgagt
     3301 acgcggaaaa catcggcatg gatctgatcc tcgaaccgtt aacgccgtat gaatcgaacg
     3361 tcgtgtgtaa tgctaatgat gtgcttcatg cgctggcgct ggtgccttcg ccgcgcctgt
     3421 tcagcatggt cgacatttgc gcgccgtatg tccaggcgga accggtgatg agttatttcg
     3481 acaaactggg tgataaattg cgtcatctgc atattgtcga cagcgacggg gccagtgaca
     3541 cacattacat tcccggcgaa ggcaaaatgc cgctgcggga gctgatgcgc gatattatcg
     3601 accggggcta tgacggttac tgcacggtgg aactggtgac gatgtatatg aacgagccca
     3661 gactctatgc ccgccaggcg ctggaacgct ttcgcgcgct gctgccggag gatgagagat
     3721 gaaaaccctg gcgacaatcg gcgataactg cgtcgatatc tacccgcaac tgaataaagc
     3781 gttttctggc ggtaatgcgg tcaatgtggc ggtgtactgc actcgctacg gcatacagcc
     3841 gggacccatt acctgggtgg gtgacgatga ctacggcaca aagctgaagc aggatctcgc
     3901 ccgcatggac gtcgatatca gccatgtcca tacgaaacac ggcgttaccg cacaaactca
     3961 ggtggaactg cacgacaatg atcgcgtttt tggcgactac accgaaggcg tgatggccga
     4021 ctttgccctg agtgaagagg attacgcctg gctggcgcag tatgacattg tgcacgcggc
     4081 aatctgggga catgcggaag acgcattccc acagctgcac gctgcgggca aacttaccgc
     4141 tttcgacttc tccgacaagt gggacagccc gctctggcag acactggtgc cgcatctcga
     4201 ttttgccttt gcctccgcac cgcaagaaga cgaagcgctg cgtctgaaga tgaaagcgat
     4261 tgttgcccgt ggcgcaggaa cagtgattgt cacgctgggt gaaaacggca gcattgcctg
     4321 ggatggcgcg cagttctggc gtcaggctcc tgaaccggtg acggttatcg acaccatggg
     4381 tgccggagat tcgttcattg ccggattcct ttgcggctgg tctgcgggga tgacattacc
     4441 gcaggcgatg gcgcagggaa cggcgtgcgc ggcgaaaacc attcagtacc acggtgcctg
     4501 gtaggtataa cgttggcgtg agcatcttca cgccaacgtg ctgttacttg ccggaaaacg
     4561 accctataat ccgagtaatt cattctttat ttcagggtcg attatgtcag ctacggaccg
     4621 ctactctcat caactcctct acgctaccgt ccgccagcga ctgctggatg atatcgcgca
     4681 gggggtttac caggccgggc aacagatccc taccgaaaac gagctttgta cacaatataa
     4741 cgtcagccgc attaccattc gcaaagccat cagcgactta gtggcagacg gcgtactgat
     4801 ccgctggcag ggaaaaggca cctttgtaca aagccagaaa gttgaaaacg ccctgcttac
     4861 tgtcagtggt tttaccgatt ttggcgtctc acaaggcaag gcgacgaaag agaaagtgat
     4921 cgaacaggaa cgggtcagcg ccgcgccgtt ttgcgaaaag ctgaacatcc ccggaaacag
     4981 cgaagtgttc catctctgcc gggtgatgta tctcgataaa gagccgctgt ttattgatag
     5041 ttcatggatc ccgctgtcgc gttatcctga ctttgatgag atttacgtcg aaggaagctc
     5101 cacctatcag ttatttcagg agcgttttga cacgcgagtg gtcagcgaca aaaagaccat
     5161 cgatatcttt gccgccaccc gcccgcaggc aaaatggctg aaatgcgaac tgggcgaacc
     5221 gttgtttcgc atcagcaaaa tcgcctttga tcagaatgac aaaccggtgc acgtctccga
     5281 actcttctgc cgcgccaatc gcatcacctt aactattgat aataaaagac attaaccgta
     5341 ggccggataa gatgcgccag catcgcatcc ggcgatgctg gcgcgttgaa ttttacatcc
     5401 cgtacgttcc tctcacccta accctctccc caaaggggcg aggggaccgt tcagtacgga
     5461 atttagcttg acggtgctat ccagcattaa atactggcaa tactctcacg caaaatccgc
     5521 agcaccgtct cttcaccgct gcgattcggg ttaatgcgaa tcgcacaatg ttctgattgt
     5581 ggattcgcct ggcgaaacgt tccggaaagg cgataaaaga gcggcgggat ttcatatttc
     5641 gactctgcac ccaccgggta aggcaaggca ccgcgctttt gcgcctcttc cagcactctg
     5701 gcggcaatcg gctgatgaaa ctcgacaatc aacaccttcg actgcgcatt agcaatcacc
     5761 gcgcttttca cttccggcac cgcaccaccg ttaagcaaag ccagcaaccg ttcagatacc
     5821 cctgcctgca ccgcgtgcat cactggcgca aacaccagac cacgcaagac ttccagcgcc
     5881 tgtgcgccct ggatctggct accgccggag taaagcgtgg cgcgaatacg gttgataaca
     5941 tcagcatcgc cgaccactgc accaacaccc tctggcccaa atagcttgaa gcaggaaaat
     6001 gtcgagatat tcgcgccgca ttcacagccg attcgcgcca ccttcatcac cgcatagttg
     6061 tcatcggtta acgctggaac acctgccgcg cgcaacgttg ccagcacatc tgccagcacg
     6121 tagctgtcct gcggctgctg gcgcgtatgc tgcaccagcg ccgcatccgg ttgttgctcg
     6181 tcgacgacct gcttcagtgc cgacaggtca ttgaaatcaa cagtaataag cgtcagcccc
     6241 atctgctcaa taataacccg tgtcgtcggg taaacaggcg cgtcatgcac cagaagacgc
     6301 tgccccggtt tgagcaaagc cgccaacccg gcgcgaatcg cgccagtccc cgcgccctgc
     6361 accagcgccg cagcctgtgc gtgaaatgca tcagccagca cctgttccac acgctgggta
     6421 acgcgcggtt gattcagtcc tggcgttaag cctaaatcac cgccagtaag aaactcgctg
     6481 ccggggaaat ggcgacaaat gctatccacc agcgcaaact gcttttgctg cgcctcaata
     6541 atcgtcaggc tttgcagagg aaacgtcttc ataggtgcct tccttaagcc ggaacgaaca
     6601 ggccaagcca gtaaagaaca ttcagcagaa taccggtgat cattactgcc acgaccggtg
     6661 ccgccatttt ctgtaccgga cggcccaggg attcgttgag gaagtaaatg gcaaccgcga
     6721 tagagaatcc ggtataaccc gccatcttaa ttgctgcgaa aatcgaaccg accagcagcg
     6781 ccacttccat cagcatattc atggcgttac ggatgttatc cgacgcatta cgcaccgacg
     6841 ggtagcgtcc cagccatttg ccgatcgaac gaagcagcaa gacttccgcc gaaataacca
     6901 ctgcgcctaa taccgctgca accatcggat tcggcgagag atagcccacc gcataaacaa
     6961 aggtaaagcc cgcaactgca tacacacccg ttgctaaggc ggtggtggca atcaacggta
     7021 caaaacccag cccgcgcata aattctgcca gtgccgcctg gttgatcagc gtttgcgatt
     7081 gttccggcgt tacgcctgcg gagtacgctt tctccagagt gaagatcgac acttcactgc
     7141 cggcgaaaat cttcatgctg gcaacggcgg caatcaatgc tcccacgatg gcgatatagg
     7201 gtaagttttt gataatccgc gacgtgcgtt cttcaaacac cgaaagcccg ctggcatcaa
     7261 tatcattttc atcacgatga cgcaggtcgt gagttatcgc gatccccagc agcatcacca
     7321 tgccaataaa gatttcgatg gattcagggt taagatgtgg gaaatagcgc acgactacca
     7381 cacgggtcat cagaacaacc acggcggcga ccagactttg cttccagcca aactggtaga
     7441 aaatcgccac cagcgggaac agtgcaaaag cagaaaccac cggcgagctt aattccccga
     7501 ggctacctaa tacatccacc ggcagcgcgg tcagcagctg ctttactggc aacaggcaag
     7561 taaggatcaa tacgccccag atagcgccaa gaccaaacgc catcaggctg tttatcgcca
     7621 gtacgccgag gatatcggta ggaagaaaga gtaaccatgc gttgagcagt ccggttttca
     7681 gggtaaaaga gatccccacc gaggccacaa aaccaatgct cagaccaaaa gcgatactcc
     7741 ccgcctcgcg acggttcata tagccttcaa tcagttgcgg caggatcggg cggatgccgt
     7801 catgaaaaac agccgccgag cgatgcgcca gaagcgatgt catacccgtc aggcacgcca
     7861 ccacgataat ctgaatatac agatccatag tcgtgccttt tatttcagat ggttaatcag
     7921 catcggaatc gcgtgttcga cgtgctcaac ggaaaggcca aacgccactt ttccttcagc
     7981 gatcattttg gcgatatgct cgtctttcgc tttaatgcct ggtttggcaa tggtgcaact
     8041 tttgttatag ccaatcaccg cgatggcaat cgacaatgcc gcgcccgcac cggtattaca
     8101 cgcaccgatg tagtaatcca gttgcccgga tttcactttc attgccgctt ccatgtcgtt
     8161 gtgaatgaaa acttcaaaac tgccaggagc cgtcgcttca atggtttttt taatctgctc
     8221 acgctgtaag cctgcaacgc caatcttttt catgagtctg tccttattgg aaaaattgag
     8281 agggagtttc acgtaacatc acatccacat cggcctgact gaatcctgac tggcgcaatt
     8341 gcggaataaa ggtggtcaat aaatagtcat agccataacc accattggct tttaaatggg
     8401 agcggcgcgt aatatccatc gacagcatga cgcggttcag caacccacgg tcacgtagcg
     8461 cgtggagcat cgcaatgcgc ttttcgtccg ggtagtaact gttcttacca atggtgtcga
     8521 actgcacgta cgcgccgaga tcgatcatct tcaaaatgtt gtcgaggttg tctttcagat
     8581 cgcagtgacc aacggtgacg cgcgaaagat caactccgtg ggcttgcaac agagccagtt
     8641 gttccagccc catggtgctg aacgaagtat gcgtggagat cgggcgtccg gtctggttat
     8701 gcgccagcgc agcggcaata aacaccttct cttccagcgg cgtaatcttc ccttcgctag
     8761 agccgatctc cgcgataatc ccggctttca ggtcggtgcc atcgataccc tgttcaattt
     8821 catcgaccat ctcctgcgcc agttcctgca cgctgcgggt cgccacatgc tccgggaaaa
     8881 aagcgtcctg gtaataaccg gtgcaggcca ccacgttgat ccccgtctcg cgcattacat
     8941 cgagcataaa ttgcgcattg cgccccatgt aacggttggt catctcaatc acattacgca
     9001 cgccccgggt catcaggtcg ttcatctcct ggcaaatgaa cgcatactga tcaaggcggc
     9061 agtccacgtt gtttttaaag ccggagagat caatatgcag atgctcatgg gccagggtgt
     9121 aacccgtcgg atcaaaactc atagggtgtc tcctgcaaac cggagggagg aaagaaaaga
     9181 gcgaccattt tgtggcggtg gcgcacggaa aaattcacac acggtagccc ccacatctga
     9241 aagcgtggtg cgcacaccga gctgcgtagc tatcatccct tgctgataaa ccagcactgg
     9301 caccacttcg cgggtatggt ggctgtgacc aatggtcgga tcgttaccgt gatccgccat
     9361 cacgaccagg caatcatctg gctgcatcgc ctcaacaagc cgggcaaggt tacggtcaac
     9421 gacctgcaaa cgttcggcat aacgtgcgac gtcttctgca tgaccagcga ggtcggtttc
     9481 ctggatattg gtgcaaataa acgccgtcgg atgcgtgtta aattcgtcaa gggtgatatc
     9541 cataatccgc tggctatcca ccaggttttg ccagctcacg ccataaggat tgctgacgat
     9601 atctgccact ttacctacca gcacggttgg cacgcccgcc tcatacagtt tttgcggcac
     9661 ctgcactttt tcatcaacac cgtagcccat atgcacgacc tggaaaccgt tgtcataagc
     9721 gccagaacgc ggcgcattga tgccaataaa gcgcccttct ttgctttctg cggcatcgag
     9781 aatacgctga ctgtcggtta acaagccgcc aaatgtaatg acccgaccga cctgtacctg
     9841 ctcacgcacg atacgaccaa ttttgattgc gtcgtcaaaa gagatcacag agagattggc
     9901 ggtaatgtta tagacttggc ctaaatccgc ctcgagatta tcgccaatcg caaccgcctg
     9961 attgacccac agaaattgca gatcatcgcc acggcgctcc acctgccagc cagcggaaac
    10021 taatgcctgc tcaacacggt caatcacatc gcgaaaaggc atccgcagcg gcggtaacgg
    10081 gcgcgtgcct aaaatttcct gatgccccat aaaggtatcg ccaccttcat gttgcagctc
    10141 tgccacgccc caggttgcgg aatctgacgg ctgcatatcg cctggcgcat aacccaatgc
    10201 gttgattagc cccagcttct ccagcgttgg tagctgcaaa tgcggcaact ggctcaggat
    10261 gtgaccacag gtattcgctc ccgcatcttg cggacgcacc agcgtgacat ctttcattgc
    10321 ccctacgcca aagctatcaa tcactaacac cacaaatcgc gccattacgc ccccagagag
    10381 tttccaagac tgtcataacg accaacgatt ttcggttcgc cacggtgaat accggacacc
    10441 agcaccacat cgctacgggt gacaaaaatc tgcgtgcgga aacagagcac cactgcactg
    10501 cttaccggaa actcgcctgc cagcgacagg gtgtagtcga tactgctgtc atccacagtt
    10561 ttgagattgg tttcagtaat cttttgattt tctggcgtaa acaccaacgc atgttgcgca
    10621 tgaccacgac ggtagtaacc gccgccgtag cagtagctgt cgccacggaa atgatgggag
    10681 atttcactca accagagcat cgcgatacgt tcagactgat cgccctgctg gtttgccgga
    10741 atagtgcccg tcagcgcatg accgggttcg gcatgagtca caccgtattg cgccagtaat
    10801 ggcagcgaag tgcagctggt cgctgaaggc gcgttcagtt gctcaatcac aataccagat
    10861 ttcgccagtt gatcccgtgc ttgtaccagc gtgtgaagat tcggtgtcgg caaaactttc
    10921 ccggcagcct catcccaaag caggcaaggg aaatgggtaa gtccggctaa atgcagcccc
    10981 ggcagtttct taatttcagc aaccacctcg tgcagcgaat gctgtacaaa accgctctcc
    11041 tgccccggat aaagaaaatc atcgtcgcta taaactttga gcagcacaga ctggattcgc
    11101 ccggccttca ccgccgccgc agaaacttcc cgcgctttgt cgagagtaaa cacggtgatg
    11161 acgtcggtgc cctgttcaac ggcgtcagca acctgatgac aagggatttg taccagatgc
    11221 ccctgatgcg ccaccggcaa accagcgcgg cgcatgactc gcgcctcttt gtaatccacc
    11281 gccacaatgc cgctgtagcc caacgccagc agtttttccg ccagccatgg gttacgaccg
    11341 aattgtttgg tcatcagata cagttcaatg ccgtaaagcc gcgccgtctc aatcagccgc
    11401 ttaccgtttt ccagtatctg atccacgtcg atcacccagc tgtccggggc gattttgccc
    11461 tgctgccaca aacttagtgc ggcggaaatc agcgccgggt tctggcgttt caatgcttct
    11521 acaaacatct tgttgcctcg tttctgttcc ttgaattaaa tattcatttt tttgaatatt
    11581 taggttgtgc gacgtggatt tgtcgggagc ttcaaacctc ttcgttagca gccatccata
    11641 atccataaag gttggccagc aaatagcctt cttcacacgg gttaacttcc agtgcgaatt
    11701 ccttcaacaa tacctgatgc aattgcacaa cagcttgcca gtggctggat tgcgccagct
    11761 ctgccagcaa ttcgttatcc agcggctcta tttcttcacc acggcgactg cgcatcagtg
    11821 cactcgccat atgtgtaatc gccatcgttc cttgctcact gcgcactggc agatggcact
    11881 ctgtttccag tacgttgacg acctgcatca tgcctttgca gatgtcctta tcaataacgc
    11941 ctgcctcgca aagcaggttg agtctggttt ccatatcaga tgcctttcgt ttaataactc
    12001 ggttcctgtt ccccactcac cacgcgttgt tgatgggcga gcagggcgtg tttatctaca
    12061 acggcacgca gcagttgttg cattgggtaa tcatcggctc gcgtcaggac cacggcttcg
    12121 gtggcctgta aaaatcgcgg atcgtctgtg agcggcgtcg cctctaatcc cagcatggtc
    12181 agttcgtttt ccgccaccac gttccagata accgcatcga catcgccttt aacaatgcgt
    12241 tgtaaactct cgtgataaga gagatcgaca cgttccacat cactatcgcc aaaaaagaca
    12301 tcggtcatga ttttctgatc cgccgaacgg ttatccagcc ccacgcgctt cacgtttgcg
    12361 gactcgcctt tacggcaaat caactggtgc tcgccaacgt aggtgtgcgg ccccaactcc
    12421 agcgcgaggc aaaacccttt ttgcgtgaga taactttccg ccgccagtcg ggaaaccacc
    12481 gccatgtcat acacgccgtt aagtaaacac tccacgcgaa tatccgcgcc gcgcatatgc
    12541 gcatagtaaa aaggaatgcc atcaaactgg gctttcaatc cgctcgctaa accttcgtac
    12601 aagcgggtat agggcaaagg catcgcacat accacgttgt tgatatccac atgactcagc
    12661 aatgctttgt tatccatctc gaccagataa ctgccattgc gcccacggcg ttcaatccgt
    12721 atcgctccgc ttgattccag cgttttcaac gcggcctgcg tcaggccaac ggatgaacgg
    12781 cattcagttg ccagctcatc tatggttttc aacctattac cgcatttttc acccaacaaa
    12841 taacgggcca gcgtcgtgat aacgacgcct tcttttttga taaacgttcg acgcataata
    12901 tattttcaat aaaatgaatg tttatatctt cattaaaatg aagataagtg gcaactgtga
    12961 gatgtggaaa cggcgaggcg cttcacattt tttcggatta ttgcaaatga ggataaaaaa
    13021 accgggtttc cccggtttca gagtgatgac gaagtgattt attcgacaat gcgagcaaag
    13081 ggtaattccc actggcgctt gcctgctccc ctcaccctaa ccctctcccc aaaggggcga
    13141 ggggaccgtc cgagcgtttt gatattacgg cttcgccaca aaaccaatcg cttcgtacac
    13201 cgctttcagc gtacgggaag cgtgtacact ggctttttct gcgccgtctt tcatcacctg
    13261 ttgcaggaag gcttcatcgt tgcggaaacg gtgatagcgt tcctgcaatt cagtcagcat
    13321 accggaaacg gcatcagcca cttcaccttt cagatgacca tacatcttgc cttcgaactg
    13381 tttttccagt tctggaatgc tctggcccgt taccgcagaa aggatatcca gcaggttgga
    13441 aacgcccgct ttgttctgca catcgtagcg aactaccggc ggctcgtcgg agtcagtgac
    13501 cgcgcgtttg attttcttca ctaccgattt cggatcttcc agcaggccga taacgttatt
    13561 gcggttatcg tcagacttgg acatcttctt ggtcggctcc agcagcgaca ttacgcgcgc
    13621 gccagatttc ggaataaacg gctccggcac cttaaagatc tcgccataca gcgcgttgaa
    13681 acgctgggcg atatcgcgac tcagttccag gtgctgtttc tggtcttcac ccaccggtac
    13741 cagattagtt tgatacagca ggatgtccgc agccatcagc accggatagt caaacagacc
    13801 agcgttgatg ttctcggcat aacgcgcaga tttatcttta aactgggtca tgcggctcag
    13861 ttcgccgaag taggtatagc agttcagtgc ccagcctaac tgcgcatgtt ctggtacgtg
    13921 ggactgaaca aaaatggtgc ttttctcagg atcgatacca caagccagat acaaggccag
    13981 cgtatccagc gtcgctttac gcagcttctg tgcatcctgg cgcacggtga tcgcgtgttg
    14041 gtcaacgata cagtaaatgc aatggtagtc atcctgcatg tttacccact gacgcagcgc
    14101 acccatgtag ttaccaatgg tcaattcacc tgagggctgt gcgccactaa aaacgatggg
    14161 cttagtcatt tttcgattcc tgattttcgc tatgcggaag ccctaatgcg ggcagaaggt
    14221 catttataga ctgataaatt acatcaggct ggctgagatc gatagcctcg ccgtagttat
    14281 atccgtaggt taagccaact gatgggcaac ctgccgcttt tgccgcctga atatcattgc
    14341 gtgagtcgcc gacaaacagc atctgttgtg gggcaattcc catccgctca gccaccagta
    14401 acagcgggtc cggatgcggt tttttgtttt gcacatcatc gccgccgata accacgctaa
    14461 agtatttggc aatatctaag gcttcgagca gcggcgcgac gaacggcgtc ggtttgttgg
    14521 tgaccaggcc tagcggcagg cctttagcct gcaacgcgcc caacgtatcg gcaacgtgcg
    14581 ggaacaaaaa cgtcccctct tcggcaacct cgccatagta gcgatcgaac agtttacgca
    14641 gaatacgtac ctgttcttct gccggaatgt cgtcatcaac gggcggttta cccattgttt
    14701 tacgctgagt cgcacgttcc tgacgcgccc aggtcaatgc gcgctccatc agaacatctg
    14761 cgccgttacc aatccaggta ataacgcgtt cttcacctgc gacgggcaac tccagcgcat
    14821 acagcgccat atctaccgca gcagcaagac cgggagcact gtcgaccagc gtaccatcaa
    14881 gatcaaaagc gacgccgcga atatcttcaa acttattcat gacttacctt tgccagttca
    14941 ctgcgcattt catcaatgac ttttttgtag tctggctggt cgaagattgc cgaaccggcg
    15001 acgaacatat ccgcgcccgc cgcagcgatt tcgccaatgt tgttcacctt cacgccaccg
    15061 tctacttcca gacgaatgtc aaagccagac tcgtcgatac gacgacgtac ttcgcgcagt
    15121 ttatccagtg tttgaggaat gaacgactga ccgccgaaac caggattgac ggacatcagc
    15181 aggatcacat ccagcttatc catcacgtaa tccagatagc tcagcggtgt cgccgggtta
    15241 aataccagtc ccgctttaca gccattttct ttaatcagtt gcagcgtgcg gtcaacatgc
    15301 tcggaggctt ctggatgaaa ggtaatgatg ctggcaccag cggcagcgaa atcaggcaca
    15361 atgcgatcga caggtttcac catcaggtgt acgtcgatag gggcggtaat gccatagtta
    15421 cgcaaggatt tcagcaccat tggcccaatc gtcagattgg gaacatagtg gttatccatg
    15481 acgtcaaaat gcacgacatc agcgccagct gccagggctt ttgcggtatc ttcacccagg
    15541 cgggcaaaat cagccgacag aattgagggg gcaatcaaat actgtttcat ccgcttctcc
    15601 ttgagaatta ttttttcgcg ggtgaaacga ctcctggttt gtacaaagcc agcagttcgt
    15661 ccaccttttt acgtgtgccg ccgttgctgc ttatactgcg tcgaactttg acgacatgca
    15721 attttgcgcg ctgataccac tcacgcgtta acatcgtatc gtgattggag atcagcactg
    15781 gaatatggcg ctcaaccaga ccttcggcga tctccgccag atgcgcttgt tgttcaagcg
    15841 taaaactgtt tgtgtgatac gccgtaaagt tggcggtcgc agacagcggt gcataaggcg
    15901 gatcgcaata gacgacggat gcatcatctg cgcgcgccat gctatcggcg taagactcac
    15961 aatagaaaaa ggcattctgc gctttttcag cgaagtgata caactctgct tccgggaaat
    16021 agggtttttt gtagcggcca aacggcacat taaactcacc gcgcagattg taacgacaca
    16081 ggccgttgta accgtagcgg ttcaaatata aaaacagtac cgcccgacgg aacggatcct
    16141 ggcttttgtt gaactcttcg cggaactgat agtaaacctc ggcgcaattt gtttcgggaa
    16201 caaacagctc gcgtgcggcc tgtacgtact catcagtacg catcttcaca atgttataga
    16261 gactgatcag gtcgctattg atatcggcaa ggatataacg agaaaagtcg gtgttgagaa
    16321 acaccgaccc ggcacctaca aaaggctcaa ccagacattc gcccttgggc aaatgccgtt
    16381 taatatcatc aagcagggga tacttgcccc ctgcccactt caaaaaagcg cgatttttct
    16441 tcatgctgac taactaatta caccttctcc ggctgtggag aaagctccga cagcatccag
    16501 cgcttcaggc aactcccgca acgataaacc attgcgggag ataaccttga ttacttcaga
    16561 tcggcctgta cctgacgcag cggtttcgcc cacgggtttt tggcctggac atctgctggc
    16621 aatgtagata ccgctttttt cgcctcttct ttcgaagcgt acacgccaga aaccaggaca
    16681 taccacggct gaccattacg cgtcgtttca tagacaacgt agtttttcag attctctttc
    16741 ttcgcccaac cgttcaggtt gccgtagtta gaggaactgc tcagctgcag agtgtaatgg
    16801 ctggacggtg ccgatttcaa cgaaccaaca ttacctgcgg tcttccctcc agcggctggt
    16861 gttgccgtgg tttgcgccgg ggatgccgtc tgtactggag ccgtagtcgc cgtctctttt
    16921 gttgctggcg tagaagtcgc agccggtgcc ttcgtgctcg ccacaggggc agctggttca
    16981 gtacgcttcg gcgtctgtgc taccggcttc ggctccgttt tcacggttgc ttgcggtttt
    17041 ttcggttcaa tcactgcctg ctggcgagct ggacgcgtag tggccggacg ttcagcggtt
    17101 tgcgttttcg ccgtttcacg ggatgcattg ccattgcgaa caggcgcgac agtcgcgggt
    17161 tcggtcggca atgtggaatt gaccgccaca ttgttcaact gttgctgatt ttgtggttgg
    17221 gtcagcgcat tgttcaggtc accctgcact tcaacacgtt gttgaccatc cgttgccgcc
    17281 ggggtttgcc cttgagtcgg cgtagaagag atcggcggca gagaaacatc ctgctgagta
    17341 ttttccgcag acgtggttcc cggcgctggc tgcacgccat tggcctgatc ggtcgcattg
    17401 ccagcaagat caatactctt ctcgccagac gcggtttgat cgctggaaga ggtcgagggg
    17461 gcttttagcg cagaaccgat accgatgatc aacagcagta gaaccagaat gccgacgccc
    17521 atcatcatat actgacgaga agcgggttta ctggctgctt ttttgcgctt acgcggacga
    17581 cgctctacgc gctcttcatc cacggtttca tcttcggatt cgtcaatttc ttcttcgatt
    17641 tccggttcct cattgcgctc tttttgcgca cgagtcggac ggcgatcgtc agtgtcatca
    17701 agttcaatat catcaaaatt gatctgcggt tcgccacgtt cagtacgctc agaacgttca
    17761 gaagattgac gagaacgacc agtacgacga tcgctgggat cgggtttcag ctcgtcttct
    17821 ggtttgaatt catccattta acaccccact aaaaggttaa tgcttaccac gttgcaatta
    17881 acctgaagct aatagaccgc ttgataagcg gcctgacctt tcttgttgtt acgctgattg
    17941 acaatcggca atggcgttaa gaacaagctc gtgcgaaacg ccgctgcgaa cttcactctt
    18001 accaattgcc aacggaagaa ttaagcgcat ctctcccgca aggactttct tgtcacgcag
    18061 catatgcggt aaatacgcct gcgcggacat ttcgcgcggc ccattgaccg gtaacccagc
    18121 ccgcgtgagc agggttataa tacgctgcgt ttcggcagaa ctaaactgcc cgagacgttc
    18181 cgacgtccgc gccgccatca ccatacccgc agcgaccgct tcaccatgta accaattgcc
    18241 ataccccatt tcagcttcaa tggcatgacc aaaggtgtgt cccagattca gtaaagcacg
    18301 taacccggtt tcgcgctcgt cggcggcgac aacttctgcc ttcagttcac aacaacggcg
    18361 aatacagtac gccattgccg gaccgtccag acgcaacaac gcatccagat tctcttccag
    18421 ccagttaaaa aacgcaccgt caagaataat gccgtatttg atgacttctg ccagccccga
    18481 cgctaactca cgcgggggaa gcgttttcag acagtcgaga tccaccacca ctgaagcagg
    18541 ctggtagaac gcgccaatca tgtttttacc gaggggatgg ttgaccgcag ttttgccgcc
    18601 aacggaggaa tcgacctgcg acagtaacgt cgtcgggact tgaatgaaac gaacaccgcg
    18661 ctgataactc gccgccgcga agccggtcag atcgcccact acgccgccgc caagcgccac
    18721 cagcgtagta tcgcgaccat gcggcttttg taacaacgcc gtaaagacgg tatcgagtac
    18781 agccaggctt ttatactgct cgccgtcagg gaggataacg ctatcgacgt taacacccgc
    18841 ctgttcaagt acgccgcgga ccttatcgag atacagagga gccagggttt cgttggtgac
    18901 caacatgacc tgctcgcccg atttcagcgg taagaatgaa gctggttcat taaacaaacc
    18961 agatgcgatg gtaattgggt aactacgttc cccgagagta acgacaatcc tctccataac
    19021 gcgacatcca ccttaattac tgtacccgca gacgagtgta tataaagcca gaattagttg
    19081 ctttccagca tgtgaataat ctggtttgca accactttag cgctttgatc atcagtacga
    19141 atggtcacgt cggcaatctc ttcatacagc ggattgcgtt cattggccaa cgcttccaga
    19201 acttcacgcg gcggtgtttc aacgtgcagc aacgggcgtt ttttatcacg ctgcgtgcgt
    19261 gcaagttgct tttcgatggt cgtttcaaga taaacgacaa cgccacgagc ggaaagacgg
    19321 ttacgcgttt cacgggattt cacagagccg ccgccagtag ccagcacaat accctgtttc
    19381 tcggtcaact cattgatgac cttttcttcg cgatcgcgga agccttcttc gccttctaaa
    19441 tcgaaaaccc agcccacatc agctccggtt cgtttctcaa tctcttgatc ggaatcgtaa
    19501 aattccatat tgagttgttg agctaactgg cgcccaatag tgctttttcc ggcacccata
    19561 ggcccaacca gaaagatatt gcgtttctct gccatttttt cggtactact aagactattc
    19621 gttaatgata aacccgcttc gctcagagag cgccgcagga catgaactga aacctcataa
    19681 gatattgcga gagtcagact gaaaattatc tcaatactcc agcgggtttg gcaactgaat
    19741 aaatcaccca tccccctgca tatctggtcg ctgcaagcgc gctgccttgc taccaccgct
    19801 ctggcgataa atcaccgggt aagattagcg taaaaaagac agcaaaatgc cgcctgaatg
    19861 ataaatcatc atcatggaac gcaggcagcg aagagcacca actctcaaat cggtactcct
    19921 tgtatgctaa atacctgcgc gcgtcaaata gatgaaacac gttcagcata aaaacattac
    19981 cgcttttacg gctgtttact cactggaaac cagtcgtggc gtgataaaca ccactaactc
    20041 gcgtcgttca tcttcttttc cgtcatgacg aaataattgc ccgaaccagg gaatgtcgcc
    20101 aagcaacggt acgctatcct gacccgattt atttttacgg gtaaaaatgc cgcccagcgc
    20161 caacgtttct ccgcttttga cctcgacctg cgtttcgatc tcctgcttat caatcgccag
    20221 cacttcgcca tcggcctgct gtagcacctg ccccggaacg ttctggctga tgtgtaattt
    20281 cagccggatg cgaccttttt gtaacaccgt gggcgtgacc tccatcccca ggacggcctc
    20341 tttaaattcc accgacgtcg cgccactttc cccgctggaa acctgatatg gaatttcgct
    20401 cccctgttta atgctggcag gctgaagatg tgaggccagc agacgcggac tggcgataat
    20461 atccagctgc tgtttttgtt cgagcgcaga aagctcaaga tccagcaagc gtccgttgat
    20521 gcgcccaatg ttaaaaccga catgcgttgt cgccgtcgct acggagaggt cgctaccaag
    20581 cgtggtgact tgcccaacgc caccagcgtg ttgcgcatcg gccagcgtcc atttcacgcc
    20641 taactcacgc aaactttttt cattaatggt gacaatatgc gccgacagct caacctgccc
    20701 gaccggcaga tccatttgcg ctacccactg ttcaagcgcg cttaacgccg ttttgttatc
    20761 tcgtagcaaa aggcgattgg tgcgtttatc gacggtcata ctccctttgg cactcagtag
    20821 cttctccccc gctttcgcca gttctcccgc gtcggcgtat tgcagggtta tactgcgatt
    20881 ttccagcggc agatttgcct gcgcccgcgc ctgctccgcc tcctggcggg cgatattgtt
    20941 attctgccag gcaatggaat gcactgagag aatgttgtct tcctgccgcg ttatcagtcc
    21001 ggcgcttttc actacagttt gtagtgcctg cttccaggga acatctgtta gatgtaacga
    21061 caccgtaccg ctgacgtctg gcgacacgac caggttcaac ttctcctgtt cagccagcgc
    21121 ctgcaacacc tgagctaccg gaacgtcatc caccatcagc gtcacttttt gcggctttgc
    21181 cgcctgtacg ccgggtatca gcatcaacag tagtgcggct atccattgct tcatttgtat
    21241 ctccttgccg ttgccacaac cattgtggcg gttcgcagtt tgtcccggtt cccagcgtta
    21301 gtgcgtctgg cgtcagctgt aaaattgtcc agccgttttc cagcacatcg ttttgctgca
    21361 cccgtcgcca tttcttttgc ccgtctttta ttacaccgat gatgcgctcg cctcgcccta
    21421 ccatcccctg atagcgccac tggctaagtt cgctaatccg gcatagatct tccggcggtt
    21481 taaaagggtc acgcataccg gttaaaaggc acaatgcaat acctgccaac aaccagcgtt
    21541 taaccctcat gcggcgtctc cagttgtagc gtgaacaaaa gatcatcacc ttccacgctt
    21601 aacgaaaaac ggctcacgct gacgttgcgc tctgccagcc gtgtaaatgc cgacggcact
    21661 gcttcccaca gcgttttcaa cgccaactca cctccctgcg cggatggatg ccaggaaacc
    21721 agttgcgcgc cggataactg aaaatccaga ggcgaaaagg gcagcgtttt ttcctcgctg
    21781 aagggagtgg tgtctaccag gcgatacagt gcggcccact gctggtgatg acttgcccgt
    21841 agtcgaatta atgcgtcgcg ctcttcatgg tgtgtcgatg atagaaaaat gagcgtaact
    21901 aacatcagca accagaatgc ccagcaaagc tggcggaggc ggggtgatgt ggcgaaccac
    21961 cagtcaaaga acatgttcat cgctaacctt ccttgttaac tgatactcaa attgccagcg
    22021 tccctgcgca tcctgctgcg tggccccccg ctgattgaga tgaaaagaag catcctggcg
    22081 aagtgacgtt tctagtgcgt ttaacgcggt aatgcttgtt gtaagcccct tgatctccag
    22141 cgttccctgc tgccagctta tcgttgtcag ccaggcgtgt tcaggtaaaa gagccgccag
    22201 tgcttccagc gcagattgcc agtcgcgggt aaattgtcgc tggcgctgcc tttgcaaacg
    22261 ctgctcacga atttgttggc gctccagcaa acgtggtttc gttatctgta agctgtgggc
    22321 gagttgttgt tccccctgaa gcaaaacggc gtctacgcga gcttcggcgc tgcttgtcag
    22381 acgcagtatt agcgttatcc cgacggccag cagcagaggc gcaacgaaca tcagcaacca
    22441 gaaacgcaga aaagcggtcc ggcgttgctg tcgccagggc aaaaaattaa ttggcgggtt
    22501 catcagtacg ccttcccaag cgccagcccc agcgcgatgg caaagtctcc acctgacggc
    22561 ggtagcggcg gctgacgaac agaaacggcc tcccagggat caaatccccc ttcgccacat
    22621 atcgcgacgc tttcgggatc aacggataac gctgccgcca gctccttcgc gctagtcatc
    22681 cccaccgcca gtttgcgccc ccagcgatag cgtgtcgccc acagccactg ttcgttatca
    22741 cgccaggcca gacattgctg atgagaaggt aaaaaaggca ggaatcgctg taatgcactg
    22801 gcatccgggg tgatcgcact aacatgaaca cgcaaccttt ctgccagagt aagcagcgtt
    22861 gccagctctt tgctttgcgc ggcagtcacg ttataagcgg ggctgagtga gtcttcgcta
    22921 taatcgaagc gcagggagtc cggatccata tccagctcgc gggccatcgt ccctgacagc
    22981 caggccgttt gctcccgctc accaagggac atcgacgggc gcggaaatga ccgctgtaat
    23041 gtgcgactgg cgggaaacgc caacataatg tgatgacgct gcggcagttc gcgactccaa
    23101 ggtaacaacg ttttagccag ctgctgcgca tcaacaatcc gcccatcttt gataatgtcg
    23161 ttctccagcg gcaaccgcca ccagcgttgc aaaaagcatt cttttgcgcc ccgtacgatc
    23221 gcaaccgcta ccgcttcttg ctgttgtaaa tgcaaaccaa tttgccagat cttaaatgcc
    23281 attgtgatga tctccttatc acccgtcact ctgacgggta tatcaatgcg tctggcttgc
    23341 ctttatacta ccgcgcgttt gtttataaac tgcccaaatg aaactaaatg ggaaatttcc
    23401 agtgaagttc gtaaagtatt ttttgatcct tgcagtctgt tgcattctgc tgggagcagg
    23461 ctcgatttat ggcctatacc gctacatcga gccacaactg ccggatgtgg cgacattaaa
    23521 agatgttcgc ctgcaaattc cgatgcagat ttacagcgcc gatggcgagc tgattgctca
    23581 atacggtgag aaacgtcgta ttccggttac gttggatcaa atcccaccgg agatggtgaa
    23641 agcctttatc gcgacggaag acagccgctt ctacgagcat cacggcgttg acccggtggg
    23701 gatcttccgt gcggcaagtg tggcgctgtt ctccggccac gcgtcacaag gggcgagtac
    23761 cattacccag cagctggcga gaaatttctt cctcagtcca gaacgcacgc tgatgcgtaa
    23821 gattaaggaa gtcttcctcg cgattcgcat tgaacagttg ctgacgaaag acgagatcct
    23881 cgagctttat ctgaacaaga tttacctcgg ttaccgcgcc tatggtgtcg gtgctgcggc
    23941 acaagtctat ttcggaaaaa cggtcgacca actgacgctg aacgaaatgg cggtgatagc
    24001 cgggctgccg aaagcgcctt ccaccttcaa cccgctctac tcaatggatc gtgccgtcgc
    24061 gcggcgtaac gtcgtactgt cgcggatgct ggatgaaggg tatatcaccc aacaacagtt
    24121 cgatcagaca cgcactgagg cgattaacgc taactatcac gcgccggaga ttgctttctc
    24181 tgcgccgtac ctgagcgaaa tggtgcgcca ggagatgtat aaccgttatg gcgaaagtgc
    24241 ctatgaagac ggttatcgca tttacaccac catcacccgc aaagtgcagc aggccgcaca
    24301 gcaggcggta cgtaataacg tgctggacta cgacatgcgc cacggctatc gcggcccggc
    24361 aaatgtgctg tggaaagtgg gcgagtcggc gtgggataac aacaagatta ccgatacgct
    24421 gaaggcgctg ccaacctatg gtccgctgct gcctgccgca gtcaccagcg ccaatcctca
    24481 gcaagcgacg gcgatgctgg cggacgggtc gaccgtcgca ttgagtatgg aaggcgttcg
    24541 ctgggcgcgt ccttaccgtt cggatactca gcaaggaccg acgccgcgta aagtgaccga
    24601 tgttctgcaa acgggtcagc aaatctgggt tcgtcaggtt ggcgatgcat ggtggctggc
    24661 acaagtgccg gaagtgaact cggcgctggt gtcgatcaat ccgcaaaacg gtgccgttat
    24721 ggcgctggtc ggtggctttg atttcaatca gagcaagttt aaccgcgcca cccaggcact
    24781 gcgtcaggtg ggttccaaca tcaaaccgtt cctctacacc gcggcgatgg ataaaggtct
    24841 gacgctggca agtatgttga acgatgtgcc aatttctcgc tgggatgcag gtgccggttc
    24901 tgactggcag ccgaagaact caccaccgca gtatgctggt ccaattcgct tacgtcaggg
    24961 gctgggtcag tcgaaaaacg tggtgatggt acgcgcaatg cgggcgatgg gcgtcgacta
    25021 cgctgcagaa tatctgcaac gcttcggctt cccggcgcaa aacattgtcc acaccgaatc
    25081 gctggcgctg ggttcagcgt ccttcacccc aatgcaggtg gcgcgcggct acgcggtcat
    25141 ggcgaacggt ggcttcctgg tggacccgtg gtttatcagc aaaattgaaa acgatcaggg
    25201 cggcgtgatt ttcgaagcga aaccgaaagt agcctgcccg gaatgcgata ttccggtgat
    25261 ttacggtgat acgcagaaat cgaacgtgct ggaaaataac gatgttgaag atgtcgctat
    25321 ctcccgcgag cagcagaatg tttctgtacc aatgccgcag ctggagcagg caaatcaggc
    25381 gttagtggcg aagactggcg cgcaggagta cgcaccgcac gtcatcaaca ctccgctggc
    25441 attcctgatt aagagtgctt tgaacaccaa tatctttggt gaaccaggct ggcagggtac
    25501 cggctggcgt gcaggtcgtg atttgcagcg tcgcgatatc ggcgggaaaa ccggtaccac
    25561 taacagttcg aaagatgcgt ggttctcggg ttacggtccg ggcgttgtga cctcggtctg
    25621 gattggcttt gatgatcacc gtcgtaatct cggtcataca acggcttccg gagcgattaa
    25681 agatcagatc tcaggttacg aaggcggtgc caagagtgcc cagcctgcat gggacgctta
    25741 tatgaaagcc gttcttgaag gtgtgccgga gcagccgctg acgccgcctc cgggtattgt
    25801 gacggtgaat atcgatcgca gcaccgggca gttagctaat ggtggcaaca gccgcgaaga
    25861 gtatttcatc gaaggtacgc agccgacaca acaggcggtg cacgaggtgg gaacgaccat
    25921 tatcgataat ggtgaggcac aggaattgtt ctgattaaaa aggcgcttcg gcgccttttc
    25981 agttggctga caaagtgcat ttgtttatgc cggatgcggg tgaacgcgtt atccggctaa
    26041 caaaatcgtg aaaaatcaat atattgcagg aaacccataa gcctgataag cgttgtgcat
    26101 caggcaaact tcacgcattt acactcgccc ctgccctttc aaccattcgc gcacgaggaa
    26161 cagcgcgctg acattacgcg cttcgttgaa gtcagggtct tccagcaaat ccatcatatg
    26221 cgccagcggc cagcgcacct gtggtagcgg ctctggctca tcgccttcca gcgattccgg
    26281 gtagagatct tgcgcgacca cgatattcat tttgctggaa aagtaagacg gtgccatgct
    26341 gagcttcttc aaaaaagtca gatcgttcgc accaaatcca acctcttctt ttagctcgcg
    26401 gttagcggct tcgtagacgc tttcacccgg atcaattaat cctttcgaaa aacctaattc
    26461 gtaggattca gttcccactg cgtattcgcg gatcaggatc aggtgatcgt ccacaatcgg
    26521 cacaatcatc actgcttccc ggttggttgg acgcattcgt tcataaacac gccgcacgcc
    26581 attgctgaac tccagatcca cgctctcgac ggtaaacagt cgggaacggg ctacagtttc
    26641 aacattcaga atggtgggtt tttgtaatga tttgctcatc gtgggatcta tgctgtgaaa
    26701 tcagcggtta ttgtgcgata tcggacacgc tttcggcaat gtgaattgca tgttatttac
    26761 atttatgtaa cttaataaat aatcgtcctc aaatcaaatt aaaagtcaat aggttgaaat
    26821 aactccagga atttgctgat attccgcctt cagagggttt gctatgatca gcggttactg
    26881 tgatgtgctt aatgatgctc aagttaaact ccacgcttgc cgatagccaa ccgcagaatc
    26941 atgtattgtg tctggtgcga ctgaccacgc ctgacagact aagtaagatg gggaaagcat
    27001 gagcaccatt gtgatttttt tagctgcttt gctggcctgc tcactacttg cgggatggct
    27061 gataaaagtg cgatccagac ggcgtcagct gccctggacc aacgccttcg cggatgcgca
    27121 aacgcgtaaa ctcacacctg aagaacgtag cgccgttgaa aattatcttg agagcctgac
    27181 gcaggtatta caggtgcctg gcccaacggg agccagcgcg gcaccgatct ctctggcgct
    27241 gaatgccgaa agcaacaacg tcatgatgct gacacacgct atcacgcgtt acggcatctc
    27301 taccgacgat ccgaataaat ggcgttacta cctcgattcg gtagaagtcc acctgccccc
    27361 tttctgggaa caatacatca acgatgagaa taccgttgaa ctgatttata ccgattcgct
    27421 gccgctggtt atttcactca acggtcatac gctgcaggag tacatgcagg aaacgcgcgg
    27481 ctatgccttg caacctgttc cgtccacgca ggcgtcgatt cgcggggaag agagtgagca
    27541 aatcgagcta ctcaatattc gcaaagaaac gcatgaagaa tatgcgctga gtcgtccgcg
    27601 cgggctgcgt gaagcgttgc tgatcgtcgc ctccttcctg atgttctttt tctgcctgat
    27661 taccccagat gtatttgttc cgtggctggc aggcggcgca ttactgctgc tgggcgcagg
    27721 tctgtggggg ctattcgcgc ccccggcaaa atcctccctg cgagaaattc attgtctgcg
    27781 cggtacaccc cgtcgttggg gattgtttgg cgaaaacgat caggaacaga tcaacaatat
    27841 ttcactcggt attatcgacc tggtctatcc cgcacactgg cagccataca ttgctcagga
    27901 tctcggtcaa caaaccgata ttgatatcta tctcgaccgc cacgtagtgc gtcaggggcg
    27961 atatctttcg ctgcatgatg aagtaaaaaa ctttccgtta cagcactggc tgcgcagtac
    28021 gattatcgct gcgggctcgc tgctggtgct gtttatgctg ttattctgga tcccgctgga
    28081 tatgccgctg aaattcactc tctcatggat gaaaggcgcg cagaccattg aagccaccag
    28141 cgtaaaacaa ctggctgacg ctggcgtgcg ggtaggcgat acattgcgta ttagcggtac
    28201 gggaatgtgt aatattcgca cttccggaac ctggagcgcg aaaaccaatt cacctttttt
    28261 accgtttgac tgctcgcaga tcatctggaa tgacgcccgc tcattaccat taccagaatc
    28321 tgaactggtc aacaaagcga cggcattgac tgaagcagtt aatcgccagc tgcaccctaa
    28381 accggaagat gaatctcgcg tcagtgcctc attacgttca gcgattcaaa aatccggcat
    28441 ggtattgctt gatgattttg gcgacattgt actgaagaca gcggatttat gttctgccaa
    28501 agatgactgt gtgcgactga aaaatgcgct ggtcaatctc ggcaacagta aagactggga
    28561 cgcgctggta aaacgcgcca acgccgggaa gctcgatggc gtgaatgtgt tattacgccc
    28621 ggtgagtgca gaatcgctgg ataacctggt ggcaacctcc accgcgccgt ttatcacgca
    28681 tgaaacggcg cgagcggcac aatcactaaa cagtccggcc cccggcggat tcctgattgt
    28741 cagcgacgaa ggcagcgatt ttgttgatca gccctggcct tcggcatcac tttacgacta
    28801 cccgccgcaa gaacagtgga acgctttcca gaaactggca caaatgctga tgcatacgcc
    28861 gtttaacgcc gaaggtatcg tcacaaaaat cttcactgac gccaatggta cgcagcatat
    28921 tggccttcat ccgatcccgg atcgttccgg cctgtggcgc tatctcagca ccacattgct
    28981 gctactgacg atgctgggta gcgccattta caatggcgta caggcctggc gtcgttacca
    29041 gcgtcatcgc actcgcatga tggagattca ggcctattat gaaagctgcc tgaacccgca
    29101 actgatcacc ccttcagaaa gccttatcga ataacacgtt tgcgcggcag gttatgctac
    29161 cctgtcgcgc aaattgcttc actctggaga tttccctcat gcatatcaac attgcctggc
    29221 aggacgtaga taccgttctg ctggatatgg acggcacgtt gctcgacctc gccttcgata
    29281 actatttctg gcaaaagctg gtgcctgaaa catggggcgc caaaaacggg gttacgccac
    29341 aggaagcgat ggaatatatg cgccagcaat atcacgacgt acagcatacg ctaaactggt
    29401 actgtcttga ttactggagt gagcaactgg gtctggatat ctgtgcgatg accaccgaga
    29461 tgggaccgcg tgccgtactg cgtgaagata ccattccgtt tcttgaggca ctgaaagcca
    29521 gcggtaagca gcgaattttg ctcaccaatg cgcatccgca caacctggcg gtaaaacttg
    29581 agcataccgg tctggacgca caccttgatt tattactttc cacccacaca tttggttatc
    29641 cgaaagagga tcagcggtta tggcatgcgg tggccgaagc tacgggtctg aaagctgaaa
    29701 gaacgctgtt tattgatgac agcgaagcga ttctcgatgc tgccgcgcaa tttggtattc
    29761 gttactgcct cggcgtgact aatcctgatt ccgggattgc cgagaaacag tatcaacgcc
    29821 atccgtcact gaatgactac cgccgcctga tcccctcgct aatgtgaagg agacgccatg
    29881 aaagagaaac ctgctgttga ggttcgactg gataaatggc tatgggctgc ccgtttttat
    29941 aaaacccgcg cgctggcccg tgaaatgatt gaaggcggta aggtgcatta caacgggcag
    30001 cgcagcaagc cgagcaaaat cgtcgagctg aatgccacgc tcactctgcg ccagggaaat
    30061 gacgaacgca cggtgattgt aaaggcgatt actgaacagc gtcgccccgc cagcgaggca
    30121 gccttgctgt atgaagagac tgcggaaagt gtagagaaac gcgaaaaaat ggcgatggca
    30181 cgtaaactta atgccttaac catgccgcac ccggaccgac gcccggacaa aaaagagcgc
    30241 cgcgacctgt tacgatttaa acacggcgac agtgaataac tgtcacctgc aagagagatg
    30301 attatgccgc aacatgacca attacatcgc tatctgtttg aaaattttgc cgttcgcggc
    30361 gaactggtaa ccgtttcgga aaccctgcaa cagatccttg agaaccacga ttatccgcag
    30421 cccgttaaaa acgtgctggc agaactgctg gttgcgacca gcctgttaac cgctacgctg
    30481 aagtttgatg gtgatatcac cgtacagctg caaggcgacg gtccgatgaa tctggcggtt
    30541 attaacggta acaataacca gcaaatgcgc ggtgtggcgc gcgtgcaggg cgaaattcca
    30601 gaaaatgccg acctgaaaac gctggtcggc aatggttacg tggtgatcac cattaccccg
    30661 agcgaaggcg aacgctatca gggcgtggtt ggtctggaag gtgataccct ggcggcctgc
    30721 ctggaagatt actttatgcg ttctgaacag ctgccgacgc gcctgtttat tcgcaccggc
    30781 gacgtagacg gcaaaccggc tgcaggcggt atgttgttgc aggtaatgcc tgcgcaaaat
    30841 gcccagcagg acgactttga ccacctggcg acgctaaccg aaaccatcaa aaccgaagaa
    30901 ctgctgacct taccggcaaa cgaagtgttg tggcgtttgt atcacgaaga agaggtgacg
    30961 gtttacgatc cgcaggatgt ggagttcaaa tgcacctgct cgcgtgaacg ttgcgccgat
    31021 gcgctgaaaa cgctgcctga tgaagaagtt gatagcatcc tggcggaaga tggcgaaatt
    31081 gacatgcatt gtgattactg cggtaaccac tatctgttca atgcgatgga tattgctgaa
    31141 atccgcaaca acgcgtctcc ggcagatccg caagttcatt aatgagtatg tccggcagag
    31201 agggctctgc cggattttac gacgtaatgg cctgatgtgc tgcgcttacc aggcctgtga
    31261 aataaatcgc aaattattgt tggtcagata cagcgtcgta tccgttaaaa gagttgtcac
    31321 ttacttaagt ttatttccag cgtttcacca tctaccgtca gatcgcgcca cgctttcggc
    31381 ctcgtcccca gttttccatc cttcgcagct gtgagtaatt tttctttcgt tatttcgttc
    31441 ggaagtaaac tcatatcaag cgcgtcccat tcttttctgt ctgagtcaaa gccatagaca
    31501 atgactctgt catcattaaa agcaaacagg atccgctccg cctgaccatc actattcaga
    31561 tcttgctcga ccaggacgca ggcgtctttt tcaatacagg tcatcacgtt atagcgatcc
    31621 tggattaagg ccgaccagaa tgtcgcatcc ggtttaacag aaccaggggc aattaacaca
    31681 ttttcggcta atactttttc cgatacctgt tcctgaagat gttgctctcc atctaacgcc
    31741 atcagcagat cccgcgcgcg tttcgggtct ttcataaacc cggcatcgct tttcagcgac
    31801 tcaagcgcag cacgtccata gcgaccgctc tgctcgagca tgtagatagt tacctggtct
    31861 gacgtgtttt tgccgctctg ataacgcgcc atatggctgt tcacgctaat gcgcatactg
    31921 tccagcaccg gcgaattaag aagcaccagt atcaccaaca ccaataacga aaccgcaagg
    31981 ttcactttgc cctgaagaac aacgggattt tgccctttac gccagacgat gctgacaaaa
    32041 taccccagcg accacaccag taacaccagc accgccagca cgccctgcaa gcggtcgaca
    32101 gtccagccgt actgagcgac ccgcagccat aacgcccagg cggcgacgaa cacatacagc
    32161 ggagcaacca gcaaagcggt tttaatcagg caacgcaacg gccctgtcca gggaagtgac
    32221 gctttttgcg gatcgcggac aatagccatt aagatcaatt gcaaaaaggc cagcgttaac
    32281 agcaacccgg cggcggagat gtggcgagaa atcgcgctca ggcccgtaaa cggcagagtg
    32341 atgataaaca tcagggttag caatgatact aacggcagca accccgtggc gattaacgtg
    32401 aacaactttt gaatagagtc gattaaacgt gactgtgttc gcgcgaggat caccgccagc
    32461 gcggtaacca gacctaacgt gagataaata aaccagtcgg ttgcaaaaaa gagcgtattg
    32521 aaaaacgtga tgccaacaag tttaaacaac tcactccaca gtaaaagcac cagccacgtc
    32581 aagccattgg cgagaaaaat caccagtaat ataagtacgt tatgccatac tgactggtaa
    32641 aaatagcgat agcggctact gtcattgcgg atgcgaaggc tttgttgtat ccacggtagc
    32701 aacaacattg ccatcagcag cagatagcaa ccaaaatccc aaagcgcctt ttcagctctc
    32761 caggggttca tgccatcagt ttgccacttc agccagccgc tcatccccag cgtggcaata
    32821 aacaccagcg ccagccatcc ccaaaggcgc ttctgtttaa aagagatcac ggagaagagt
    32881 aaaacggatg aaaacgcaac ggtcgcaggc acgccataaa caatccaact gtgatttttt
    32941 ccggcaagcc aggcgatcaa caggtagcaa accagcccct gaagtaatcc ggtagcaatc
    33001 atcccccagc gtgttgccgg tgaaagttca acgttatcca tgttggttat ccagaatcaa
    33061 aagtgggtta attatcgcat ccgggcagta gtattttgct tttttcagaa aataatcaaa
    33121 aaaagttagc gtggtgaatc gatactttac cgattgaatt tgcatcaatt tcattaagga
    33181 atgcgattcc actcacaata ttcccgccat ataaactaag atttaacctt ttgagaacat
    33241 tttccacacc taaaatgcta tttctgcgat aatagcaacc gtttcgtgac aggaatcacg
    33301 gagttttttg tcaaatatga atttctccag atacgtaaat ctatgagcct tgtcgcggtt
    33361 aacaccccca aaaagacttt actattcagg caatacatat tggctaagga gcagtgaaat
    33421 gcgcgttaac aatggtttga ccccgcaaga actcgaggct tatggtatca gtgacgtaca
    33481 tgatatcgtt tacaacccaa gctacgacct gctgtatcag gaagagctcg atccgagcct
    33541 gacaggttat gagcgcgggg tgttaactaa tctgggtgcc gttgccgtcg ataccgggat
    33601 cttcaccggt cgttcaccaa aagataagta tatcgtccgt gacgatacca ctcgcgatac
    33661 tttctggtgg gcagacaaag gcaaaggtaa gaacgacaac aaacctctct ctccggaaac
    33721 ctggcagcat ctgaaaggcc tggtgaccag gcagctttcc ggcaaacgtc tgttcgttgt
    33781 cgacgctttc tgtggtgcga acccggatac tcgtctttcc gtccgtttca tcaccgaagt
    33841 ggcctggcag gcgcattttg tcaaaaacat gtttattcgc ccgagcgatg aagagctggc
    33901 aggtttcaaa ccagacttta tcgttatgaa cggcgcgaag tgcactaacc cgcagtggaa
    33961 agaacagggt ctgaactccg aaaacttcgt ggcgtttaac ctgaccgagc gcatgcagct
    34021 gattggcggc acctggtacg gcggcgaaat gaagaaaggg atgttctcga tgatgaacta
    34081 cctgctgccg ctgaaaggta tcgcttctat gcactgctcc gccaacgttg gtgagaaagg
    34141 cgatgttgcg gtgttcttcg gcctttccgg caccggtaaa accacccttt ccaccgaccc
    34201 gaaacgtcgc ctgattggtg atgacgagca cggctgggac gatgacggcg tgtttaactt
    34261 cgaaggcggc tgctacgcaa aaactatcaa gctgtcgaaa gaagcggaac ctgaaatcta
    34321 caacgctatc cgtcgcgatg cgctgctgga aaacgtcacc gtgcgtgaag atggcactat
    34381 cgactttgat gatggttcaa aaaccgagaa cacccgcgtt tcttatccga tctatcacat
    34441 cgataacatt gttaagccgg tttccaaagc gggccacgcg actaaggtta tcttcctgac
    34501 tgctgatgct ttcggcgtgt tgccgccggt ttctcgcctg actgccgatc aaacccagta
    34561 tcacttcctc tctggcttca ccgccaaact ggccggtact gagcgtggca tcaccgaacc
    34621 gacgccaacc ttctccgctt gcttcggcgc ggcgttcctg tcgctgcacc cgactcagta
    34681 cgcagaagtg ctggtgaaac gtatgcaggc ggcgggcgcg caggcttatc tggttaacac
    34741 tggctggaac ggcactggca aacgtatctc gattaaagat acccgcgcca ttatcgacgc
    34801 catcctcaac ggttcgctgg ataatgcaga aaccttcact ctgccgatgt ttaacctggc
    34861 gatcccaacc gaactgccgg gcgtagacac gaagattctc gatccgcgta acacctacgc
    34921 ttctccggaa cagtggcagg aaaaagccga aaccctggcg aaactgttta tcgacaactt
    34981 cgataaatac accgacaccc ctgcgggtgc cgcgctggta gcggctggtc cgaaactgta
    35041 atgatttgaa gctggagaat atctatccag tatcttatag aaagcaaaac gggaggcacc
    35101 ttcgcctccc gtttatttac ccttcttttg tcgtgccctg cgcccgcgtt accggcactg
    35161 gcagccaggc gcgaatggaa agcccgcccc gctcgctggt gccaagctcc agcatcccgt
    35221 tatggttatc cacgatacgc tgcacaattg ccagccctaa tcccgtgccg ctaatggtgc
    35281 gcgcactgtc gccgcggaca aacggctgga acaggtgctt acgttgttcc ggcgcaattc
    35341 ccggaccgtc atcttccacc tggaaccagg cgcgattcgg ctccgttccg ctgctgactt
    35401 tgatccagcc attgccataa cgggcggcgt tgaccaccat attcgccacc gcgcgtttga
    35461 tcgacagcgg gtgcattttc acttcaatgc tgccggggta aagcgcggtt tcaatttccc
    35521 gctcatagcc actttcggca gcaatcacct caccgagtac tgcattaaga tccgccattt
    35581 ccatcggcat ctcctgcccg gtgcgcaggt agtcgataaa ctgctcaatg atggcgttgc
    35641 actcttcgat atctttattg atcgattctg ccagatagcc atcctgctcg ctcatcatct
    35701 cagtcgccag gcgaatacgc gtcagcggcg tgcgcaagtc gtgacttacc cccgccatca
    35761 gcagcgtgcg gtcatccgcc agttgcttaa caccagccgc catatggtta aaggcacggg
    35821 taacggaacg cacctccgaa gcgccatact cacgcagcgg cggcggaata atccctttac
    35881 caacctgcaa ggctgcgtgt tcgagatcga ccaacggtcg gttctggata cgaataaaca
    35941 gccacgcccc gcctatcgcc aatagcataa tcgccagcgt atagcggaac agcggagaga
    36001 aatcgccctg atgaatttcg gtcagcggca cgcgtaccca gatattgggc gacagccagg
    36061 ttttcagcca gacgacaggc gaacttttgt tgacctcaac gcgcacttcc gtcgggccgc
    36121 ccagttgctg tgccatctga tggcttaaga attcatagtg ttgcgcccaa cgcagacctg
    36181 cctcttcggc agcctcgttg gagtagagag agatccccag ctcacggtag atctcccgac
    36241 ggaaagcggg aggcacaacc aactgcgtgc cgtcctccag ttgcagtttg tcggtcatca
    36301 acatacgcac ttcgtacgcg aggactttat taaactgctg gaggctcggc aaaatcgcga
    36361 agttcagcac caccagataa gtcgtcacca ggctggcgaa cagcaaggtg acgatgagca
    36421 ataacgtacg ggcaaatgaa cttcgtggcg agaagcgcaa tcgcctcatg ctttagagcc
    36481 gtccggtaca aagacgtagc ccaggcccca aacggtctga atgtaacgcg gatgcgctgg
    36541 atcttcttct accatgcggc gcagacgcga aatctgcacg tcgatggagc gttccattgc
    36601 ggaatattca cgaccacggg caaggttcat cagcttatcg cgggagagcg gctcacgcgg
    36661 atggctgacc agtgccttca gtaccgcaaa ctcaccgctg gtgagcggca tcggctcatc
    36721 ttcgcggaac atttcgcgcg taccgaggtt aagtttgaac ttaccgaaag caattaccgc
    36781 ctcttcctgt gacggtgcgc ctggcagttc gttcgcctga cgacgcagca ccgcacgaat
    36841 acgggccagc agttcacgcg ggttaaacgg ttttggaatg tagtcgtcag cgccaatctc
    36901 caggcctacg atacggtcca cttcttcccc tttcgccgtc accataatga tcggcatcgg
    36961 gttgctctga ctacgaagac gtcggcaaat cgacaagcca tcttcaccag gtaacattaa
    37021 atccagtacc ataagatgga aagattcacg agtcagcagg cgatccatct gttctgcatt
    37081 agcgacgctt cgaacctgga agccttgttc ggtgagataa cgttccagca gcgcacgcag
    37141 gcgcatgtcg tcatcgacca ccagaatctt gtagttctct tgcattgttt gtactcccaa
    37201 aggttcgcaa caatttgtaa gcgtgtattc ttaaaaaagc tcacgttcgt caccagctaa
    37261 atctggtatg aatttcagcc taaattgtta caaagcatat taaacagcag cttaagtata
    37321 caatttattc ggcgaaacat tattgattct gttgatatga tcacgttata cccaatgtgc
    37381 gcattatcaa acagacaaag ggaatcaacg agatgaaaac gcccctggtt acccgggaag
    37441 ggtatgaaaa actcaaacaa gagcttaatt atctctggcg tgaagaacgc ccggaggtca
    37501 caaaaaaggt gacctgggcc gcaagtctgg gcgaccgcag cgaaaatgct gactatcagt
    37561 ataataaaaa gcgtctgcgt gaaatcgacc gtcgcgtgcg ctatctcact aaatgcatgg
    37621 aaaatctcaa aatcgtcgat tactcccctc agcaggaagg caaagtcttt tttggcgcgt
    37681 gggtggagat tgaaaacgac gatggcgtga ctcaccgttt ccgtattgtc ggctacgatg
    37741 aaatttttgg ccgtaaagat tacatctcta tcgattcccc gatggcccgc gcattgctga
    37801 aaaaagaagt cggcgatctg gctgtggtga atactcctgc cggggaagcg agttggtatg
    37861 ttaatgctat cgagtacgtg aaaccgtaag gaagagtctt aacctcctgc cgatggctgg
    37921 catttttgcc agccagtccg tataactatc ccctgatttt tgatccgaaa agatgaactc
    37981 aaaccatgat gaatgattcg ttctgccgca ttattgcggg tgaaattcag gcgcgcccgg
    38041 aacaggttga cgctgccgtt cgcctgcttg acgaagggaa taccgtgccg tttatcgcac
    38101 gttatcgtaa ggaaatcacc ggcggtctgg atgacacgca gctgcgtaat ctggaaacgc
    38161 gtctgagcta tctgcgcgag ctggaagaga gacgtcaggc gatcctcaaa tccatttccg
    38221 agcaaggcaa actcaccgat gatctggcga aggccatcaa cgccacccta agcaaaaccg
    38281 aactcgaaga cctctacctg ccctacaaac ctaaacgccg cacccgcggg caaatcgcca
    38341 ttgaagcagg gcttgagccg ttggctgacc tgctgtggag cgatccgtca cacacgccag
    38401 aagtcgcggc tgcacaatat gttgatgccg ataaaggcgt ggcagatacc aaagccgcgc
    38461 tggacggcgc gcgctatatc ctgatggaac gttttgccga agatgccgcg ctgctggcga
    38521 aagtgcgtga ttatctgtgg aagaacgcgc atctggtctc aaccgtggtg agcggtaaag
    38581 aagaggaagg ggcaaaattc cgcgactatt tcgatcatca cgaaccgttg tccacggtgc
    38641 cttctcaccg cgcgctggcg atgttccgtg ggcgtaacga aggcgtactc cagctttcgc
    38701 tgaatgccga tccgcagttc gatgagccgc ccaaagagag ctattgcgag caaatcatca
    38761 tggatcacct tggcctgcgc ctgaacaatg ccccggcgga tagctggcgc aaaggcgtag
    38821 tgagctggac ctggcgcatc aaggtgctga tgcatctgga aaccgaactg atgggtaccg
    38881 tgcgcgaacg cgcggaagat gaagcaatca acgtctttgc ccgtaacctg cacgatctgc
    38941 tgatggcggc ccctgccgga ctgcgtgcaa cgatgggcct cgatccgggt ctgcgtactg
    39001 gggtaaaagt ggcggtggtc gatgccactg gcaaactggt agcgaccgat accatttacc
    39061 cgcacaccgg acaagccgca aaagcagcga tgactgttgc tgcgctgtgt gaaaagcata
    39121 acgttgaact ggtggcgatc ggcaacggta cagcttcccg cgaaaccgaa cgtttctatc
    39181 tcgacgtgca gaagcagttc ccgaaagtga ccgcacagaa agtgatcgtc agcgaagctg
    39241 gcgcgtcggt ttactcagct tccgagctgg cagcgcagga gttcccggat ctcgacgttt
    39301 cgctgcgtgg tgcggtatct atcgcccgcc gtttgcagga tccgctggcg gagctggtga
    39361 aaatcgatcc gaaatctatc ggcgtaggtc agtatcagca tgacgtcagc cagacgcaac
    39421 tggcccgcaa actggatgca gtagtagaag actgcgtaaa cgccgttggc gtcgatctca
    39481 acaccgcttc tgttccgcta ttaacccgcg tggcgggcct gacgcgcatg atggcgcaaa
    39541 acatcgtggc ctggcgcgat gagaacggcc agttcaagaa ccgtcagcaa ctgctgaaag
    39601 tcagccgtct ggggccgaaa gccttcgagc agtgcgcggg cttcctgcgc attaaccacg
    39661 gtgataaccc gctggacgcc tctaccgttc acccggaagc ctatccggtg gtggaacgca
    39721 ttctggcagc aacacagcag gcactgaaag atctgatggg taacagcagc gaactgcgta
    39781 acctgaaagc gtctgacttt actgatgaaa aattcggtgt gccgacagta actgacatca
    39841 tcaaagagct ggaaaaaccg ggtcgcgatc cgcgtccgga atttaaaacc gctcagtttg
    39901 ccgatggcgt cgagacaatg aacgacctgc aaccgggtat gatcctcgaa ggcgctgtca
    39961 ccaacgtcac caactttggc gcgtttgtcg atattggcgt gcatcaggac ggcctggttc
    40021 acatctcttc attgtcgaac aagtttgtgg aagatccgca taccgtggtg aaagcgggcg
    40081 acattgtgaa ggtgaaagtg ctggaagtgg atcttcagcg taaacgtatc gccctgacca
    40141 tgcgtctgga cgagcagcct ggcgaaacca acgcccgtcg cggcggcggt aatgaacgcc
    40201 cgcaaaacaa ccgcccggca gccaaaccac gcggtcgtga agcgcagcct gccggtaata
    40261 gcgcgatgat ggatgcgctg gcggcggcaa tgggcaaaaa acgttaaacg ccctgattga
    40321 cccctaaacc tgcgcgataa ggtgtccacg ccgcatctgg caacgaacgc cttgtctgac
    40381 ataaaagtgc cggagaatat ctccggcctt tttattccac aaccaaactc ataatatatt
    40441 ccggcaatat ttatcacttc attaacaact gaaaccttaa ttaaacatta gccagtccgg
    40501 gtaattcact attcgaatta tattttcgct gcgatataac cttgagccac atcaacattg
    40561 agtcagatta ttattcaaac caacattcgc acacatttta agtattgctg atagaaacca
    40621 ttctcattat cattgtgttg ttgattattt aatctctcct ttgttggcaa atcatctggt
    40681 ctcatgtcgc tgtcaaacgc cccatgaggt agttatccag ttaatgagaa acaagtaggc
    40741 acctatgcaa tacactccag atactgcgtg gaaaatcact ggcttttccc gtgaaatcag
    40801 cccggcatat cgccaaaaac tgctttctct tggcatgtta cctggctcct cttttaatgt
    40861 ggtgcgcgtc gctccactcg gcgaccccat tcatatcgaa acccgtcgtg tgagcctggt
    40921 attacgcaaa aaagatctgg ccttattaga agtggaagcg gtttcctgtt aatacggtga
    40981 taacaacaat gaaaaaatta accataggct taattggtaa tccaaattct ggcaagacaa
    41041 ccttatttaa ccagctcact ggcgcacgtc agcgtgtagg taactgggct ggcgttaccg
    41101 tcgaacgtaa agaagggcaa ttctccacca ccgatcatca ggtcacgctg gtggacctgc
    41161 ccggcaccta ttctctgacc accatctcat cgcagacctc gctcgatgag caaatcgcct
    41221 gtcactacat tttgagtggc gacgccgacc tgctgattaa cgtggtggat gcgtctaacc
    41281 ttgagcgtaa cctgtacctg acgctacaac tgctggaact cggcattccc tgcattgtgg
    41341 cactgaacat gctcgacatt gccgagaagc aaaatattcg tattgaaatt gatgctctgt
    41401 cggcgcgtct tggctgccca gtgatcccgc tggtttcaac ccgtggtcgc ggtattgaag
    41461 cgctcaagct ggcgattgat cgctataaag ctaacgagaa tgtggaactg gtgcattacg
    41521 cacagccgct actcaacgaa gcagattcac tggcaaaagt gatgccttcc gacatcccgc
    41581 tgaaacaacg tcgctggctg ggcctgcaaa tgctggaagg cgatatctac agccgcgcct
    41641 acgccggtga agcgtcgcag catctggatg ccgccctcgc ccgtctgcgt aatgagatgg
    41701 acgatccggc gctgcacatt gccgatgcgc gttaccagtg cattgctgcc atctgtgatg
    41761 tggtaagcaa caccctgacg gcagaaccca gccgtttcac cactgcggta gataaaatcg
    41821 tgctcaaccg tttcctcggt ctgccgattt tcctctttgt gatgtacctg atgttcctgc
    41881 tggctatcaa catcggcggg gcgttacagc cgctgtttga cgtcggctcc gtggcgctat
    41941 ttgtgcatgg tattcaatgg attggctaca cgctccactt cccggactgg ctgactatct
    42001 tcctcgccca gggcctgggt ggcggcatta acaccgtgct gccactggtg ccgcagattg
    42061 gcatgatgta cctgttcctc tccttccttg aggactccgg gtatatggcg cgtgcggcgt
    42121 ttgtgatgga ccgtctgatg caggcgctgg gcttgccggg gaaatccttt gtgccgttga
    42181 tcgtcggttt cggttgtaac gtaccgtcgg taatgggtgc acgtacgctt gatgcaccgc
    42241 gtgaacgtct gatgaccatc atgatggcac cgtttatgtc ctgcggcgcg cgtctggcta
    42301 tcttcgcagt attcgcggct gccttcttcg ggcagaacgg tgcgctggcg gtcttctcgc
    42361 tgtatatgct gggtattgtg atggcggtgc tgactggcct gatgctcaag tacaccatca
    42421 tgcgcggtga agcgacgccg tttgtcatgg agttgccggt ctatcatgta ccacacgtta
    42481 aaagcctgat tatccagacc tggcagcgtc tgaaaggctt cgttctgcgt gctggtaaag
    42541 tgatcatcat cgtcagcatt ttcctgagcg ctttcaacag cttctcgctg agcgggaaaa
    42601 tcgtcgataa catcaacgac tcggcgctgg cttccgtcag ccgggtgatc accccggtct
    42661 tcaagccgat tggcgtacat gaagataact ggcaggcaac ggttggcctg tttacgggcg
    42721 caatggcgaa agaagtggtg gtgggtacgc tcaacaccct ctacaccgca gaaaatattc
    42781 aggacgaaga gttcaatccg gcggaattca acctcggtga agagctgttc agtgcggtag
    42841 atgaaacctg gcagagcctg aaagacacct tcagccttag cgtactgatg aaccccattg
    42901 aagctagcaa aggcgacggc gaaatgggta ccggggcgat gggcgtgatg gatcagaaat
    42961 tcggtagcgc agctgccgca tacagctacc tgattttcgt cctgctgtat gtaccgtgta
    43021 tctcggtgat gggggctatc gcccgtgaat caagccgtgg ctggatgggc ttctccatcc
    43081 tgtgggggct gaatatcgct tactcactgg caacattgtt ctatcaggtc gccagctaca
    43141 gtcagcatcc aacttacagc ctggtgtgca ttctggcggt tatcctgttt aacatcgtgg
    43201 ttatcggtct gctgcgccgc gcgcgtagcc gggtggatat cgaactgctg gcaacccgca
    43261 agtcggtaag cagttgctgc gcagccagca ccaccggtga ttgccattaa tggcttcact
    43321 tattcaggtg cgcgatttgc tggcgttacg gggccgtatg gaagcggccc agataagcca
    43381 gacattgaac actccacagc caatgattaa cgccatgctg caacaactgg aaagtatggg
    43441 caaagccgtg cggattcagg aagaacctga cggctgcctc tctggcagtt gtaaaagctg
    43501 cccggaagga aaagcctgtc tgcgcgagtg gtgggcgctg cgttaacctt actccatcgc
    43561 ctgttttgga aagcccggta tgcgtctgca tccgggcttt tttgcgtgcg gctttccata
    43621 aaaatgcaac tcttgcagca cggcgtaagt tcctttgaaa gcatctcgca gggatgaaaa
    43681 ctcgctaata cacaggagtg aagtggcgcg tagagtcgcg gcattcaaac aacaggtgaa
    43741 ggaacgccat gagcaaaaag cagagttcca ccccacacga tgcgctgttc aaactctttt
    43801 tacgccaacc ggacacggct cgtgattttc ttgcgtttca tttaccggca cccattcacg
    43861 cgctttgtga tatgaaaacc ctcaagctgg agtcgagcag ctttattgat gacgatctgc
    43921 gtgaaagcta ttccgatgtg ctgtggtcgg tgaaaacgga acaaggacca ggatacatct
    43981 attgtctgat tgaacatcaa agcacctcaa acaaactgat cgcatttcgc atgatgcgtt
    44041 acgctattgc cgcaatgcaa aatcaccttg atgccggata caaaacgttg ccgatggtgg
    44101 tgccattgtt gttttaccac ggtattgaaa gcccctatcc ctattcgctg tgttggctgg
    44161 attgtttcgc cgatcccaaa ctggcaaggc agctttatgc ctccgcattt ccgctgattg
    44221 atgtcaccgt catgcctgat gatgaaatca tgcagcaccg acgtatggcg ctgctggagt
    44281 taattcaaaa acatattcgt caacgcgacc tgatggggca ggtagagcaa atggcctgct
    44341 tattaagtag tggatacgct aatgacagac aaatcaaagg gctgtttaat tacatactgc
    44401 aaaccggcga cgcggtacgt tttaacgatt ttatcgacgg cgttgccgaa cgttcaccga
    44461 aacacaagga gagtttaatg acaattgcgg aaagattgcg gcaggagggg gaacaatcca
    44521 aagccctgca tatagccaaa ataatgcttg aatccggagt ccctcttaca gacatcatgc
    44581 gctttaccgg ggtgtcagaa gaagagttgg ctgcggcgag ccagtaaatt tctgtctcgc
    44641 catttcaaaa gttgcctaca ccctctgctt caacgccacc agcaggtgac aaaactcgac
    44701 cggatgcgaa ataaacggcg catgggccgc tttggcgaag atatatgatt cgctgtgagg
    44761 ccaaagttta tccagcatcg gcaccacttt gcgcggcacc agaccgtcga gatagccata
    44821 caatcgcaaa aacggcatgg gcacgttttg cagcggctga cggagatcga ccgttttcag
    44881 gatttccagc ccgccattaa gcacgtcaac ctccggcatc ggtaacgcca gaacggtttt
    44941 cttcaacgcc cgcgcatcct ggcgcgccgt ttcagtcccc atggtttgta acgccaggaa
    45001 ccgctccact gtacgctgaa aatcatcact gagttgctgc tgaaatcccg ccagcacgtc
    45061 cggttttatc cccggccact cgtcacgagc actaaaacaa ggtgacgacg ccacggtgac
    45121 cagcgcctga acacgctcgg gatgggttaa cgcaatctgg cttgccacca gcccgcccag
    45181 actccagcct aaccaaatgg ctttatcagg tgcctgttgc agcacggctt cggccatatc
    45241 agcaagtgac agcgcaccaa atccccggct acgcccgaag ccgggcaggt caacaaggtg
    45301 cagcgtaaaa tgcgagctaa gttcctcgtc aatgcaacgc cacacttcgg cattcagtcc
    45361 ccatccgtgc agcagcacaa gatgaacatt cccctgacct ttggtctgcc accagatgtt
    45421 attcatccgc tattgttctc ttttgactta caaggatgaa catatgctaa cagtaccggg
    45481 attatgctgg ctatgccgaa tgccactggc gttaggtcat tgggggattt gttcggtctg
    45541 ctcacgcgct gcccgtacag ataaaacgtt atgcccacaa tgtggattac ccgccacaca
    45601 ctcacatctt ccctgcggtc gctgcctgca aaaaccaccg ccctggcaaa gactggtcac
    45661 ggttgccgac tatgcgccgc cgttaagtcc gcttatccac cagcttaaat tttcccggcg
    45721 cagtgaaatc gccagcgccc tgtcacgtct gttactattg gaagtcttac acgctcgtcg
    45781 caccaccggg ttgcaattgc cggatcgcat catcagcgtt ccgttatggc agcggcgtca
    45841 ctggcgtcgg ggatttaatc agagcgattt gctgtgtcag ccgttatcac gctggttgca
    45901 ctgccgatgg gatagcaaag ccgtcacacg aacacgggcc actgcgaccc agcattttct
    45961 cagtgcccgg ctacgcaagc gcaacctgaa aaatgccttt cgtcttgaat tgcccgtgca
    46021 aggtcgccat atggtgattg tggatgatgt cgttaccacc ggaagtaccg tcgcagagat
    46081 tgcgcagttg cttttacgca atggtgcggc gactgtccag gtctggtgcc tttgtcgaac
    46141 cttgtagagc ctcgatgatg ggcgtattat aaccaactaa aatagtcaac tattaggcca
    46201 ttactatgat ccgtatttcc gatgctgcac aagcgcactt tgccaaactg ctggcaaatc
    46261 aggaagaagg gacacaaatc cgcgtatttg tgattaaccc tggcacgcct aacgctgaat
    46321 gtggtgtttc ttattgtccg ccggatgccg tggaagccac cgacactgcc ctgaaatttg
    46381 acctgctgac cgcgtatgtt gatgagttaa gcgcaccata cctggaagat gcagagatcg
    46441 actttgttac cgaccagttg ggttctcagt taacgctgaa agccccgaac gccaaaatgc
    46501 gtaaagtggc agacgatgca ccgctgatgg agcgcgtgga gtatatgctg cagtcgcaga
    46561 tcaacccaca gcttgctggt cacggtggtc gcgtttcgct gatggaaatc accgaagacg
    46621 gttacgccat tctgcaattt ggcggcggct gtaacggttg ttccatggtc gatgtgacgc
    46681 tgaaagaagg gatcgagaag cagctgctga acgaattccc ggagctgaaa ggtgtgcgcg
    46741 atctcaccga acaccagcgc ggcgaacact cctactacta agttatcccc tcttttgtag
    46801 attgcccgat gcgacgctaa agcgtcattt cgggcctacg ttgaacatgt gccagacgtt
    46861 ggcacagcat tgctttggca aatccctccc ttcgtttccc cgcgataata tgaccaacct
    46921 ctcataattt aaatttaccc cgctctggtg attctcaaac gccagatgtt acccgtatca
    46981 ttcacatggg taccaaacat actcctgaca tctgactaca ataattagtt ttagtgggta
    47041 tcagtcgtgg tgccgcaata tctctgttcc cgattgggat aattagagtt tgtcgtcaga
    47101 aaattgacgt tacccataac aaatgaaagg ccaggtaaat catgccatta gtcattgttg
    47161 ctatcggtgt aatcttgttg ttgctcctga tgatccgctt caaaatgaac ggcttcatcg
    47221 ctctcgtcct cgtggcgctt gctgttggat taatgcaagg aatgccgctg gataaagtta
    47281 ttggctccat caaagccggt gtcggcggga cgctcggtag ccttgccctg atcatgggtt
    47341 ttggcgcaat gctgggcaaa atgctggcag actgcggtgg cgcacaacgt atcgccacca
    47401 cgctgattgc caaatttggt aaaaagcaca tccagtgggc ggtggtgtta accggtttta
    47461 ccgttggttt tgccctgttc tatgaagtgg gcttcgtgct gatgctgcct ctggtgttta
    47521 ccatcgcggc ttccgcgaat attccactgc tttatgttgg tgtaccaatg gcggctgcac
    47581 tgtctgtgac ccacggcttc ctgccaccgc atccgggccc gactgcgatt gccaccattt
    47641 tcaatgccga tatgggtaaa accctgctgt acggtactat tctggcaatc ccgaccgtga
    47701 ttctcgccgg tccggtttac gctcgcgtgt tgaaaggtat cgataagcca atcccggaag
    47761 gtctctacag cgcgaaaacc ttcagcgaag aagagatgcc gagctttggc gtcagcgtct
    47821 ggacctctct ggtgccggta gtactgatgg cgatgcgtgc gattgccgaa atgatcctgc
    47881 cgaaaggtca cgctttcctg ccggtagcgg agttcctcgg tgacccggta atggctacgc
    47941 tgattgccgt gctgattgcc atgttcacct ttggtctgaa ccgtggtcgt tcaatggatc
    48001 agattaacga cacgctggtt tcttccatca aaatcattgc gatgatgctg ttgatcatcg
    48061 gtggtggcgg tgcgttcaag caggtgctgg tagacagcgg cgtggacaaa tacattgctt
    48121 ccatgatgca cgaaaccaac atttctccgc tgctgatggc ctggtcgatt gctgccgtac
    48181 tgcgtatcgc gctgggttcc gcaaccgttg cggcaatcac tgcgggtggt atcgcggcac
    48241 cgctgattgc aacgacgggt gttagcccgg agctgatggt tattgcggtg ggttccggta
    48301 gtgtgatttt ctctcatgtg aacgatccgg gcttctggct gttcaaagag tactttaacc
    48361 tgactatcgg cgagaccatc aaatcctggt cgatgctgga aaccattatc tcggtgtgcg
    48421 gtctggtagg ctgtctgctg ctgaatatgg tgatttgata acactgactg ccggatgcat
    48481 cgcatccggc atcaaccgca ctctacttct tcttcgctgc agctctgcgc cgtctgtcca
    48541 aatccttcag caacttgttc acgccatcat cggcaaacat cgactcaagc gttgcggaaa
    48601 gcttgcgtcg ccagttttta tactggtaac tggtgccagg aatattcacc ggttcggcca
    48661 tatccagcca gtcttccggc tgtagtccta acagagcact gttactgtcg gcaatgtagc
    48721 gctgcaaacc acggttcagc gtcggcgtca tcgacatcaa cgatgcctta tgcccggcac
    48781 gttttggcag acaaccatat ttatgcagtg catccagcag cccttgcttc gccagttcgc
    48841 gatcctgata cagaccgcgc agtaccactt catccggata cagccccagg gttttgccca
    48901 gcgttagatc cccgctctcc cagtaaccgc gtagcgtagg caggtcatgt gtcgccgcaa
    48961 ccgccatcga ctgctccgga tacgctttcg gtgcacggaa cgtcttctcg tggtcgtttt
    49021 cgaaatagag cactttgtag gagtacacac cgctgctgcg cagcttaccg acaatctcta
    49081 ccggtacggt accgagatct tcaccaatca ccatacagcg atgacgttta ctttcgagtg
    49141 ccagaatcga gagcagatca tccaccggat agtgaacata cgcgccctga tctgccgtct
    49201 cgccatacgg tatccaccac aaacgcagca tcgacatcac atggtcaatt cgtaatgcgc
    49261 cgcagttttg catattggca cgcaacagct cgataaacgg ttcataggca cgcgcggtga
    49321 tgatatgcgg gtccattggc ggtaatcccc agttctgccc caacgggccg aggatatccg
    49381 gcggcgcgcc aaccgacgct ttcaggcaat atagttcacg gtcacaccag gtttccgccc
    49441 caccttccgc tacgccaacc gccagatcac gatacaagcc aatcggcatt tcatagccct
    49501 ggcttatctc ccagcaggcg gcaaactggc tgtaagccag ccactgcaac cagagataaa
    49561 aatcgacgtc atcacgatgt tcttcgcaga actgacgcac ttctggtgaa tccacgttct
    49621 gatacatctc tggccatgca ggccagcccc agcgcatttc gtcctctttc acttgctggg
    49681 catgtagcgc atcaaaggct gcctgccaga acaggctgtc gccctgctct gcaacaaact
    49741 ggcgaaacgc ggtcatctgc tcatgatcac gttgcgcgaa acctttccac gccattcgta
    49801 atgctgtcat ttttagggcg gtaaccgtgg agtaatcgac ccagtcggca tcgcgcgcct
    49861 gttgcagcgt ctgttgcgtg gtcggcaact gccaccaggc ctgagcctct tcgctaagat
    49921 ggaaatcttc aacggcgtta acgtcgatat aaatcacatt cagccaacgg cgagaagacg
    49981 ggctgtatgg gctggcactc tccggatttg ccggatagag cgcatgaatc gggttcaggc
    50041 cgatgaacga cccgccacgt tttgccacat ccaccagcat cgctttgaga tcgccaaaat
    50101 ccccaatacc ccagtttttt tccgatcgca gcgtataaag ctgaacgcag gcaccccaca
    50161 gcttttgttt attcagcaac gcctgcggtt cgtaacagcg tttcggggcg acaatcaccc
    50221 ggcaatgcgc gcgctggtcg tcctgggtga gtgtcagcgt gtgataacct tccggcagct
    50281 tcgtcggtag attgaacgct ttgccccccg ttacatggcc tttgtactgt gttccttctt
    50341 cggtggtcag cagccagcta tattcgccgc tgccctccac caccatcggc atttttttgc
    50401 cgctggtata aaccatgaca ttcgggactg gcgttaccgc cactttcgtg gcggtacgtt
    50461 gatgcatcgc gtcaagcaaa cgccgtttgg tttcggcgct aatcgactgc ggtttaccgt
    50521 aggcattgat gtaattgggg ctaatccccg ccgccagcgc ggcattatcc agacgtttgc
    50581 tttccatcga gcttccttag cgttttgcct gccagatacg agcctgataa tcgcgaatag
    50641 agcgatccga gctaaacata ccgcagcggg cggtattgag gatcgccgcg cgagtccagg
    50701 cctcctggtc gcggtacagc acatccacct gcttttgtgc ctcaacatag gctgcgaaat
    50761 ccgccatcac cagatacgga tcgccgcctt gtttgccgat actgtgcagc atctggtcga
    50821 aggcatgctt atcgccgtcg ctgtatttac cgctttccag ctctttcaat actgcgtcca
    50881 gcaccttatc tttcttccgc catttcaccg ggtcgtagcc tttggccaga atagccttca
    50941 cttgttccac ggtatgacca aaaataaaga tattttcttc accgactttc tcggcgattt
    51001 caacgttcgc accatccagc gtaccgacag taagcgcacc attgagcgcc agtttcatat
    51061 tgccggtacc ggaagcttct ttacctgcag tcgaaatttg ttcggagata tccgccgccg
    51121 ggatcagttt ttccgccgcc gaaacgcaat aatccggcag gaacaccacc ttcaacttat
    51181 cgccaaccag tggatcgttg ttgatcacgt cagccacttt gttgatcgca aagataatat
    51241 tcttcgccag gtagtagccc ggtgccgctt tcgcgccgaa gaggaagacg cgcggtacgc
    51301 gatcagcctg cgggttttca cgaatttctt tgtacaacgc cagaatatgc agcagattca
    51361 ggtgctggcg tttgtactca tgcagacgtt tgatctgaat atcgaaaatc gcctgtggat
    51421 tgatctcaat accagtacga actttcacaa actccgccag acggacttta ttcgcctgct
    51481 tgatctcgcg atattgctga cggaatttcg catcatcagc gaatttttcc agattgatca
    51541 gctgatcgag atcgttagcc cactcttttt gcagtgattt atccaacaga gccgccagtg
    51601 cagggttgca ctgtttgatc cagcgacgtg gggtaatacc gttggtgacg ttatggaatt
    51661 tgttcggcca tagctggtga tattccggga acagatcttt caccaccaga tccgagtgca
    51721 gcgccgcaac accgttcacc gcgaaaccgc caaccacaca caggttcgcc atatgcactt
    51781 gtttatcgtg caccaccgcc agtttggccc acactttttc atcgcccggc caggttttct
    51841 ccaccagcgt tttaaagcga gtattaattt cgttaataat ctgcatatgg cgcggcagta
    51901 agcctttcac cagtttcaca tcccagcgtt ccagcgcttc tggcatcagg gtatggttgg
    51961 tgtaagcgaa agttttgctg gtaatggccc aggcgtcatc ccagctcatc tggtgctcat
    52021 cgatcagcac gcgcagcagt tctggaatcg cgatagttgg gtgggtatcg ttcagctgaa
    52081 taacttcgta atccgccagt tcgtgcagtt tacgccccgc cagatgatgg cgacgcaaaa
    52141 tatccgctac cgaacaggca cactggaagt actgctgcat caagcgcagc tttttaccgg
    52201 cagtatggtt gtcgtttgga tagagaactt tggtcagttt ttccgcattg atgccctgct
    52261 gttcggcacg caggaaatca ccgtcgttaa atttagtcag atcaaacgga tgcgcgtgcg
    52321 tcgcctgcca cagacgcagc ggctgcgcca cgccattacg atagccgaca acggggagat
    52381 cccacgcttg accggtaatg gtaaactccg gctcccagcg tccgtctttc gtcactttac
    52441 cgccaatccc tacctgcaca tccagtgctt cgttgtggcg gaaccacggg tagttactgc
    52501 ggtgccagtc atccggcgct tcaacctgtt tgccatcgac aaaagactgg cggaacaaac
    52561 catattgata gttcagaccg taacccgtcg cagactgacc gacagttgcc attgagtcga
    52621 ggaagcacgc cgccagacgt cccagaccac cgttacccag cgccgggtcg atctcttctt
    52681 ccagcaggtc cgtcagattg atgtcataag ccttcaacga atcctgtaca tcctgatacc
    52741 agccgagatt caacaggttg ttgcccgtca ggcgaccaat caaaaactcc attgagatgt
    52801 agttaacatg tcgctgattc gccaccggct tggcgaatgg ctgagcacgc agcatttcgg
    52861 ccagtgcttc actcactgct agccaccact ggcgaggagt catttcagcc gcagaattta
    52921 agccataacg ctgccactga cgtgaaagcg cttcctgaaa ttgcttatcg ttaaaaatag
    52981 gttgtgacat aggagttcca cttttcttag attttcaaca caacgttatc gctagtttgc
    53041 caggctcgat gttgaccttc ctcatcctgc gggggattag gcagggagga gttgcgggga
    53101 tgagcaagga aatgtgatct caaccactta aagctagtgc aaaccacagg attagcagca
    53161 aatcaatgca atacagcgca gaaaatctgt atctaagtgc aaaaaatggc cgttgcgtat
    53221 tttcaaaaag cggaaggtaa ctctataaat taagtaaagg agtgaaacag tttcataagt
    53281 aaaatatcca gtgtgctcca tctcattctt aatagattta ttaagatcat ctttttagat
    53341 ggcactttca tcaggaatga agaagaaacc cttgcttaaa tgaatctgat gaacataagg
    53401 gaaaccagta ttcacgctgg atcagcgtcg ttttaggtga gttgttaata aagatttgga
    53461 attgtgacac agtgcaaatt cagacacata aaaaaacgtc atcgcttgca ttagaaaggt
    53521 ttctggccga ccttataacc attaattacg aagcgcaaaa aaaataatat ttcctcattt
    53581 tccacagtga agtgattaac tatgctgatt ccgtcaaaac taagtcgtcc ggttcgactc
    53641 gaccataccg tggttcgtga gcgcctgctg gctaaacttt ccggcgcgaa caacttccgg
    53701 ctggcgctga tcacgagtcc tgcgggctac ggaaagacca ccctcatttc ccagtgggcg
    53761 gcaggcaaaa acgatatcgg ctggtactcg ctggatgaag gtgataacca gcaagagcgt
    53821 ttcgccagct atctcattgc cgccgtgcag caggcaacca acggtcactg tgcgatatgt
    53881 gagacgatgg cgcaaaaacg gcaatatgcc agcctgacgt cactcttcgc ccagcttttc
    53941 attgagctgg cggaatggca tagcccactt tatctggtca tcgatgacta tcatctgatc
    54001 actaatccag tgatccacga gtcaatgcgc ttctttattc gccatcaacc agaaaatctc
    54061 accctggtgg tgttgtcacg caaccttccg caactgggca ttgccaatct gcgtgttcgt
    54121 gatcaactgc tggaaattgg cagtcagcaa ctggcattta cccatcagga agcgaagcag
    54181 ttttttgatt gccgtctgtc atcgccgatt gaagctgcag aaagcagtcg gatttgcgat
    54241 gacgtttccg gttgggcgac ggcactacag ctaatcgccc tctccgcccg gcagaatacc
    54301 cactcagccc ataagtcggc acgccgcctg gcgggaatca atgccagcca tctttcggat
    54361 tatctggtcg atgaggtttt ggataacgtc gatctcgcaa cgcgccattt tctgttgaaa
    54421 agcgccattt tgcgctcaat gaacgatgcc ctcatcaccc gtgtgaccgg cgaagaaaac
    54481 gggcaaatgc gtctcgaaga gattgagcgt caggggctgt ttttacagcg gatggatgat
    54541 accggcgagt ggttctgcta tcacccgctg tttggcaact tcctgcgcca gcgctgccag
    54601 tgggaactgg cggcggagct gccggaaatc caccgtgccg ccgcagaaag ctggatggcc
    54661 cagggatttc ccagcgaagc gattcatcat gcgctggcgg caggcgatgc gctgatgctg
    54721 cgcgatattc tgcttaatca cgcctggagt ttgttcaacc atagcgaact gtcgctgctg
    54781 gaagagtcgc ttaaggccct gccgtgggac agtttgctgg aaaatccgca gttagtgtta
    54841 ttgcaggcgt ggctaatgca aagccaacat cgctatggcg aagttaacac cctgctagcc
    54901 cgtgctgaac atgaaatcaa ggacatcaga gaaggcacca tgcacgcaga atttaacgct
    54961 ctgcgcgccc aggtggcgat taacgatggt aatccggatg aagcggaacg gctggcaaaa
    55021 ctggcactgg aagagctgcc gccgggctgg ttctatagcc gcattgtggc aacctcggtg
    55081 ctgggtgaag tgctgcactg caaaggcgaa ttgacccgct cactggcgct aatgcagcaa
    55141 accgaacaga tggcacgcca gcacgacgtc tggcactacg ccttatggag tttaatccag
    55201 caaagtgaaa ttctgtttgc ccaagggttc ctgcaaaccg cgtgggaaac acaggaaaaa
    55261 gcgttccagc tgatcaacga gcagcatctg gaacagctgc cgatgcatga gtttctggtg
    55321 cgcatccgag cacaactgtt atgggcctgg gcacggctgg atgaagccga agcgtcggcg
    55381 cgtagcggga ttgaagtctt gtcgtcttat cagccacagc aacagcttca gtgcctggca
    55441 atgttgattc aatgctcgct ggcccgtggt gatttagata acgcccgtag ccagctgaac
    55501 cgtctggaaa acctgctggg gaatggcaaa tatcacagcg actggatctc taacgccaac
    55561 aaagtccggg tgatttactg gcaaatgacc ggcgataaag ccgccgctgc caactggttg
    55621 cgtcatacgg ctaaaccgga gtttgcgaac aaccacttcc tgcaaggtca atggcgcaac
    55681 attgcccgcg cacaaatctt gctgggcgag tttgaacctg cggaaattgt tctcgaagaa
    55741 ctcaatgaaa atgcccgcag tctgcggttg atgagcgatc tcaaccgtaa cctgttgctg
    55801 cttaatcaac tgtactggca ggccggacgt aaaagtgacg cccagcgcgt gttgctggac
    55861 gcattaaaac tggcgaatcg caccggattt atcagccatt ttgtcatcga aggcgaagcg
    55921 atggcgcaac aactgcgcca gctgattcaa ctcaatacgc tgccggaact ggaacagcat
    55981 cgcgcacagc gtattctgcg agaaatcaat caacatcatc ggcataaatt cgcccatttc
    56041 gatgagaatt tcgttgaacg tctgctaaat catccggaag tgccagaact gatccgcacc
    56101 agcccgttga cccaacgtga atggcaggtc ctggggctga tttactccgg ttacagcaat
    56161 gaacaaattg cgggcgaact ggaagtggcg gcaaccacca tcaagacgca tatccgcaat
    56221 ctgtaccaaa aactcggcgt cgcccaccgt caggctgccg tgcagcacgc gcagaaactg
    56281 ctgaagatga tgggttatgg ggtgtgagtt tagccggata atgcattgga tccggtttac
    56341 atctttacat cattcaatac tcacccgcgt tacgccatct gtttctatca aaccaaaccg
    56401 caccggcaag aaacgctcca ccaccgcgat attggtcagc agatggcatg aaggatgggc
    56461 gaccttaaat tctcccgcgc ccgccagcgc catcggcagt accagttggt cggcgagata
    56521 ttcccccacc gccgctgggc ttgccaggta gcgtttcacc tctttcacta actgcgcggc
    56581 taccacctcg gcactgacgc gcttttcacc gacgacgaaa aagcgttcag tgatattttc
    56641 actttcgact tccagcgaga cggtattacc cggcccctgg tcgcgcggca ggttatgaat
    56701 attctgttca tgcagggaaa aactccccgc cagtgtagcg atttcacgct cagcaacatg
    56761 gcgcggcaca ccagctaata gaacctctcc acgcatctgc acaatgttcc cgcgctcgcc
    56821 aagttgcaag gtgttaaacg atgccacggg cgagacttcc gttgccacca cgccgcctcc
    56881 ggcaggataa aaaccgtgac gtaacagcgt ggtttgctga tgaattccta ttttcgccag
    56941 cagcggctcc agcacccggc ggataaaatc cgcaggcggg gccgacgggt tatcggtgcc
    57001 tccgctcact tcaacacgcg aaggtccatc ggcaaaccac agcgcgggca gcaccgtttg
    57061 cagcaccagc gtacagcttc cggcactacc gatagcaaag cggtactcgc cgccgcgcac
    57121 gatgccaggc cggaagacca gacgctgcga ccccagctcc gcgccttcca ccgttgccct
    57181 acaaatttcc gcagccgctt ttaccgcggt cagatgctgg cgcaacagcc ccggtttcgc
    57241 ccgcccggca cgaatgctgg tgatggtaaa tggctggccg gttatcatcg acaggctcag
    57301 cgccgagcgc aggatctgcc cgccaccttc gccctgtgcg ccatccagcg caatcatcct
    57361 tttcatcatt tatcctttta cgcacaccac ctgacgcagg gtatagataa cttccaccag
    57421 atcgctttgt gccgccatca ccgcatcaat atctttatac gccatcggga tttcgtcgat
    57481 cacttcggca tctttacggc attccacatg cgcggtggca cgaatttgat cttccacgct
    57541 gaacagtttt ttcgctttag tacggctcat tacccgcccg gcaccgtggc tgcacgaaca
    57601 gaaagattct tcatttccca gcccacggac gataaagctt tttgctccca tcgaccctgg
    57661 aataattcca tattgaccag cacgcgcaga caccgcgcct ttacgcgtca cgtagatctc
    57721 ttcaccaaag tgctgttctt tttgcacata gttgtggtga cagttgatct cttccatcgc
    57781 cagcgtttgt ggctgtctga ccgttttctg cgtaatgctc tgcaatgccg ttaccacgtt
    57841 ttccatcatc gcatcgcggt taaggctggc aaaaagctgc gcccaggcca cggctttcag
    57901 gtaatcatca aagtattccg taccttccat aaagtacgcc agatcacgcg acggcaacgt
    57961 ctcaagcgtt tcctgcatct ctttttgtgc cagatcgata aagtaagtcc cgatggcgtt
    58021 accaattccg cgtgaaccgg agtgcagcat aatccacacc tggtccgact catcaaggca
    58081 gatttcaata aagtggttac cggttcccag cgttcccagg tgtttatagt tattggtatt
    58141 caggaaacgg ggatattttt gcgttaacca ctgataaccg gcttcaagct cagcccattt
    58201 cgcatcgacg ttaacaggcg ggttttccca tgcgccttta tcacgtttac aacggccagt
    58261 ggtacgcccg tgcggcacgg ccatttcaat cgcctgacgc agctctgcca ggttttcagg
    58321 caggtcttcc gccgttaacg cggtacgcag cgcgttcatt ccacagccaa tatccacgcc
    58381 caccgccgcc ggaataatcg cccctttggt cgggatcacg ctaccaatgg tggaaccttt
    58441 acccaggtgt acatcaggca ttaccgcaat atgtttgaaa ataaacggca tcttcgccgt
    58501 attaataagt tgctgacgcg catcggcttc taccggcacg cctttggtcc acatttttac
    58561 cggggcattt tcagtggtca gtaattcgta attcattttg tttttctctt ttcgttgttt
    58621 gctgtcgtga taattgcaac cgtcgtgcca gaaaattaaa taagcagatt aattttttaa
    58681 ttcattgttt ttaaataggt taaatcttta cgtccgtaaa cggagatttt ccacaaaacc
    58741 aattcaccga taatgaaata tcgtctttta taaggatatc taagatgcgt aaaacagtgg
    58801 cttttggctt tgtcggtacc gtgctggatt atgccggacg cggcagtcag cgctggtcga
    58861 aatggcgtcc gacgctttgt ttatgccagc aagaatcgtt agtcatcgat cggctggaat
    58921 tgttgcacga cacccgctcg cgctcgctat tcgaaacgct taaacgcgat atcgccagcg
    58981 tttcgccaga aacagaagtc gtgggcgttg agattgaact gcataacccg tgggatttcg
    59041 aagaggttta cgcctgcctg catgatttcg cccgtggtta cgagtttcag ccagaaaaag
    59101 aagactattt aattcacatc accaccggca cccacgtcgc gcagatttgc tggtttctgc
    59161 tggcggaagc gcgttacctg cccgcccggc tgatacaatc ttcacctccg cgcaaaaaag
    59221 aacagccgcg cggcgcaggt gaagtgacga ttatcgatct cgatttaagc cgttataacg
    59281 ccatcgccag ccgctttgcc gaggaacgcc agcaaacgct tgattttctt aagtccggca
    59341 ttgccacgcg taacccccac ttcaaccgca tgattgagca gatcgaaaaa gtggcgatca
    59401 aatcccgcgc gccgattctg cttaacggtc caaccggcgc gggcaagtca tttctggcgc
    59461 gacgcatctt cgagttaaaa caggcgcggc atcagtttag cggcgcgttt gtggaagtga
    59521 actgcgccac cctgcgcggc gataccgcca tgtcgacgct gtttggtcat gtaaaaggcg
    59581 cgtttaccgg ggcgcgggaa tctcgtgaag gtttattacg cagcgccaac ggcggaatgt
    59641 tgtttcttga tgagattggc gaactgggcg cagacgaaca ggcaatgctg ctaaaagcca
    59701 ttgaagagaa aaccttttac ccgtttggca gcgatcgcca ggtgagcagt gtttttcagc
    59761 ttatctccgg aacggtgcgc gatttgcgcc agctggttgc cgaaggcaaa tttcgcgaag
    59821 atctgtacgc gcggatcaat ctctggacct tcaccctgcc gggtctacgc cagcgccagg
    59881 aagatattga accgaacctt gattatgaag tggagcgcca cgccacactc actggcgaca
    59941 gcgtgcgttt taacaccgaa gcgcggcgcg cctggttggc ttttgcgacc tctccccagg
    60001 caacatggcg cggtaacttt cgcgaacttt ctgccagcgt cacgcggatg gccacctttg
    60061 ccactagcgg acgcatcact ctggacgtgg ttgaagatga gataaaccgt ctgcgctata
    60121 actggcagga gagccgcccc tccgcgctta cggcgttgct gggcgcagag gcagaaaaca
    60181 tcgacctttt tgacaggctg caactggaac acgtgatagc tctctgtcgc caggcaaagt
    60241 cgctttccgc tgccggacgc ctgctttttg atgtttcgcg ccagggcaaa gccagcgtca
    60301 atgacgcgga tcggctacgc aaatacctgg cgcgttttgg tctgacgtgg gaagccgtgc
    60361 aggatcagca cagctccagt tgaatatgat ggtccgtcag cacctgcatt acgcttactg
    60421 gcggcggggc gtcggtgtag acggcatcta ccatgctgat gctgcccata ttgaccatcg
    60481 cgttacggcc aaatttcgag tgatcgacaa ccagcataac gtggcgcgag ttctcaataa
    60541 tggcgcgctt ggtgcgaact tcgtggtaat cgaactccag cagcgagccg tcgctatcga
    60601 tgccgcttat ccccagaatg ccgaaatcaa ggcggaactg ggagataaaa tcgagcgtcg
    60661 cttcgccaat gatcccgcca tcgcggctgc gtaattcgcc accggcgaga ataatgcgga
    60721 aatcttcttt taccatcaac gtgttagcaa cattgagatt gttggtgaca atacgcaaat
    60781 tgctgtgatt aagcagcgcg tgcgccaccg cttccggcgt ggtgccgata tcgataaaca
    60841 gtgtcgagcc gttggggatt tgctccgcca ctttgcgggc aatcctctct ttttcttcgg
    60901 tctgggtggc tttgcgatcg tgccacggcg tgttaaccga actggaaggc agcgccgcac
    60961 cgccatggtg acgcaggatc aggttttgct ccgccagctc attgaggtcg cggcgaatag
    61021 tctgcgggct gacggagaaa tgctctacca gctcttcagt actgacataa ccctgctgtt
    61081 taaccagttc gataataccg ttgtgacgtt gtgtttgttt catttataaa tccctggaat
    61141 tattttcgtt ttcgcgcatt gagcgaatca acaaaagcca tcgctaaacc cacggctaac
    61201 ccggcgatgt gtgctccgtt cgccatcgac atcccaaaca aatcaaacca tccggcgaca
    61261 atccagatca gcgcaaagat aattaaccca cgttgcaggt aaatgccact ttgcggatcg
    61321 cgttcaccac gtagccagac gtagcccatc agcgcataca ccacgccaga aagcccgcca
    61381 aaccacggcc cgctgaattt ttgctgcaca tagccgctta acagggcgct aatgagagta
    61441 ataacaatta gcttaccgct accgaggcgt ttttccaccg caccgccgag ataccaccac
    61501 cagagcaggt taaagaggat atgcatcagc gagaagtgca ttaacgcgtg ggtgaagtaa
    61561 cgccagaact caaatttcag tgttggatcg aatggccagg ccagccataa catcacttcc
    61621 tgatcgccga gaatttgcat ggcgataaac accaccacgc aggcgatcat caccacccag
    61681 gttaccggac ctgcgcgttc acgcaaggcg gcaaagaaag gataacggcg ataatgcagg
    61741 ccactgccgg tatggcctgc ctgccagctg gccgccagat aacgcggatc tgccgggttt
    61801 tcgagaaaac gcgccagctc cgcccgtacg cgctcggcct gggactcatc cgccagccag
    61861 acatcgcttt ggttatgttg ttgaatcgtg aggataacac cctgcgtcgc catgtaatca
    61921 acaaacgcct gcgccacgcg ggggttagca aaagaggtaa tcatcaacat cgttgctgtc
    61981 gcttattcca cacaaaaggg gacagtataa agcgttacgc gccgtacgcc acctctgcgg
    62041 gaaactgacg ttgccaggct tcaaagccgc cgtcaatgct atagaccaca tcgtagccct
    62101 gttgcagcag atactgcgcc gcgcctttgc tgctattgcc gtgataacac atcaccatca
    62161 ccggagtgtc aaagtcgtta tcacgcataa acgcgcccag cgtgtcgttg gttaaatgga
    62221 aagcctgcac cgcatgcccc atagcgaaac tctgtggatc gcgaatatcg accagcaccg
    62281 cctctttttc ctgcaacttc tggtgcgcgt cggcaacgtt aatacattcg aactgatcca
    62341 tgcgtctctc tttctttaca aacaagtggg caaatttacc gcacagttta cgtcgaagcg
    62401 gcagataaac gccataatgt tatacatatc actctaaaat gttttttcaa tgttacctaa
    62461 agcgcgattc tttgctaata tgttcgataa cgaacattta tgagctttaa cgaaagtgaa
    62521 tgagggcagc atggaaacca aagatctgat tgtgataggt ggcggcatca atggtgctgg
    62581 tatcgcggca gacgccgctg gacgcggttt atccgtgctg atgctggagg cgcaggatct
    62641 cgcttgcgcg acctcttccg ccagttcaaa actcattcac ggtggcctgc gctaccttga
    62701 gcactatgaa ttccgcctgg tcagcgaggc gctggctgaa cgtgaagtgc tgctgaaaat
    62761 ggctccgcat atcgccttcc cgatgcgttt tcgcctgcca catcgtccgc atctgcgccc
    62821 ggcgtggatg attcggattg gtctgtttat gtacgatcat ctgggtaaac gcaccagcct
    62881 gccgggatca accggtttgc gttttggcgc aaactcagtg ttaaaaccgg aaattaagcg
    62941 cggattcgaa tattctgact gttgggtaga cgacgcccgt ctggtactcg ccaacgccca
    63001 gatggtggtg cgtaaaggcg gcgaagtgct tactcggact cgcgccacct ctgctcgccg
    63061 cgaaaacggc ctgtggattg tggaagcgga agatatcgat accggcaaaa aatatagctg
    63121 gcaagcgcgc ggcctggtta acgccaccgg cccgtgggtg aaacagttct tcgacgaggg
    63181 gatgcatctg ccttcgcctt atggcattcg cctgatcaaa ggcagccata ttgtggtgcc
    63241 gcgtgtgcat acccagaagc aggcctacat tctgcaaaac gaagataaac gtattgtgtt
    63301 cgtgatcccg tggatggacg aattttccat catcggcacc accgacgtag agtataaagg
    63361 cgatccgaaa gcggtgaaga ttgaagagag tgaaatcaac tatctgctga aagtgtataa
    63421 cgcgcacttt aaaaaacagc ttagccgtga cgatatcgtc tggacctact ccggtgtgcg
    63481 tccgctgtgt gatgatgagt ccgactcgcc gcaggctatt acccgtgatt acacccttga
    63541 tatccatgat gaaaacggca aagcaccgct gctgtcggta ttcggcggta agctgaccac
    63601 ctaccgaaaa ctggcagaac atgcgctgga aaaactgacg ccgtattatc agggtattgg
    63661 cccggcgtgg acgaaagaga gcgtgctgcc gggtggtgcc attgaaggcg accgcgacga
    63721 ttacgcagct cgtctgcgcc gtcgctatcc attcctgact gaatcgctgg cgcgtcatta
    63781 cgcacgcact tacggcagca acagcgagct gctgctcggc aatgcggggg cgataagcga
    63841 cctcggggag gatttcggtc atgagttcta cgaagcggaa ctgaaatacc tggtggatca
    63901 cgaatgggtc cgccgcaccg acgatgccct gtggcgtcgc acaaaacaag gcatgtggct
    63961 gaatgcggat caacaatctc gtgtgagtca gtggctggtg gaatatacgc agcagaagtt
    64021 gtcgctggcg tcgtaaggat gatgcccggt tgaagtgcaa ccgggctatt tggattcctt
    64081 agactattca ttgtaaataa ttgaataacg gcccctgagc taaaatcgcc aaacttaagc
    64141 acgttaccca atcgccccac ttcttcctct ggtttctatt ttttctgcac caatggcaat
    64201 aatatcccaa tgcaaaacca agctgtgcac taatcgggcc gacaaaatca gtccaaggct
    64261 gcggccaaaa aggggaaaaa ttcaggggag gccagacaaa cgtcagaaag aaatttcgga
    64321 tattttccag caacgctgat ttctctggag aaatataaag aatgaaattc gagagaatta
    64381 accagaaata gaccggcgaa aagaccagcc agcaatgcag tctgtttttt aattcaggag
    64441 gcgttctgcg ggagaattgc caaccgatgc cagccattac actgaaaaaa agtgcatatt
    64501 caaccattaa ctcaagttgt aattcctgaa gagaaatacg cacaaccagg agcggaaagg
    64561 agaatattat ttctgccaga aaagttaacg tgaatataag tacggctaag acaataaccg
    64621 tcggccatac caataatggc acgataacaa aaccgagaat tacaggggga atataaagcc
    64681 ggaatttaat catttttatt ttcctggtga ttcggatgat gcgtcataca tcgcgcataa
    64741 acactcatca attatccagt cgtctaccca gacctgatgc tgtgtgccct gttctggctg
    64801 ctttggtggg ttctcaatcg cctgccgcgc gcgttctttc aagatggatt gattgttgaa
    64861 ggcccaggca ttgatttggg tggtaatgac gttatcgata tcgtcctggt tatctctgat
    64921 ataatcctca caatatctgt tcaataaaca ggcaagagaa acggtcgacc aaaagccggg
    64981 aactgaatta atggcatcca gcaatggcgc aagagcgata tcggcacgga aaaacatttc
    65041 cagcatatac tgtaattcga cagtttcatc gccggatacc caatcagtca tatactggca
    65101 ggcaaatcgt tgcaaaattt tccattcatc ctcacgccaa ttttctttac tggcaaggtt
    65161 gatacggcgc agagaaaaaa ctacgtgaat attgtcaaaa tgcccctgtg caacaaattc
    65221 cagtaaacgc ggcagaaaat agcggacctc atcgctattt tgcggatcag gccctgggct
    65281 actattccag gcattgatta aggaatacgg gatcgctcgt aacgatgtac tccgtaatgc
    65341 cttttgttcc tgctgactca ggcaatattc acaacaaacc acaaatttct gcggcacccg
    65401 ataacgacga aaggtgtgat aaatctgctc aattaatgcc tgcatggcgg ggctgggttg
    65461 catagttacc ccctcgcccg ctgatcgcgt gtaaacggtt gatagacaaa acgagcattc
    65521 gccgcgttca ggcgaataag ggcgctacgc gaaagggaaa agctggggcg aatgcaacta
    65581 ttgcagtctc gacagctttc ctctgcgcaa ttttccactg agtaatgacg cgtgtagagt
    65641 ttcagttcgg cctgcttatc gcgaaatccg cgtaaactgt tattgttatg aaatttccag
    65701 gcatctttaa agacctgaaa gatatagaca tcctcaatcc aggagtcaca ttttttaagc
    65761 gggcgctttg ctattatcac ccgctcatcc ggatttagca tcgccgtaaa ttgtacggtg
    65821 acgtcatttg tcgtgttcag gtcacattga acatctggta catcactgat ataggggtca
    65881 aatccaaaat cagcaccaca ggcacaaaga cggctaatgg tggcggtatc gagggaaaat
    65941 cccggccata cattgctgga cgttaagcca atatttaatg tgacattata tcgaagcaat
    66001 ccctcaagac cgtcagcacc gacgaacttt tccccttcat ccagggcctg gttaatgagt
    66061 tgctgaacaa tgccttcatc ccagtatgcc gatttgcagt tggcaatcgt aatatccacg
    66121 cctgtgaagg gatcagtacc ggcaaggctt gcaaataatg tattaactaa ttccattttt
    66181 attatccagc tttaaggtag cgtcttatac atcgggacat aacctacatc ttccaccaaa
    66241 ctctgtccct gcggcgttaa aaaccactcc aacaattttt gtgtctctga cgtcgtattt
    66301 tcccgcgtta ccataaaggc atcgacgata tagggatatt tgccgttgcg aatgttttcg
    66361 gcagtcggtg caataccgtt aatcgctagc agttttatat ttttatcgtc gttcatttgt
    66421 gttgcgtaat aacggaaggt gtaacctatt gcgttgtttg tattacggta ttcggcaacg
    66481 actttaatca ttccctccat catacttgcc acttctgttt cctgcggcga gatcatgcgg
    66541 acatttttca tgacctggga ttgcatcacc gtctggctac cagagtcttc cgggcgttgc
    66601 caggtctgga tctcctgatc gttaccgcca accgtgcgcc agttggtaat tgcaccactg
    66661 aagatgtcac gcacttgttg ttcggtcagg gaattaaccg ggttatctgc attgacgatg
    66721 aaaacaaacg cttcacgggc aaaaggcgtg taaatcaaag tgacgcccga ttcctccgcg
    66781 cgttttttct gcccaccgga aggttgagcc acaaagataa tatcggcatt acctttaaca
    66841 atcttgttat atgcttcggg agtacgagag ttcgccagat attcccattc gtgaaaatct
    66901 tccggcaaaa cgcttagtgc ataaaaggca gaggcataaa tggggtacgc cgccgttgcg
    66961 ccatcaagtc gcggccagtt ttgcgtgaac tgaatttgcg gtttaccacg cagtggtgtc
    67021 agttgattat tgagtttatc gggtcgccag gcccaggtat caatattttc acgcacaccg
    67081 acgccgggat taagctgttt atataaataa gcctggtaac cggcgacaaa accaagcatg
    67141 agaaggataa acaagagaga attacggcaa cgtaacccgg acgtgcccgt taaaggatat
    67201 ctggtcggcc agccgtaccc cagcgaaaaa ctgatatgcg cgccaacagg tataatgact
    67261 aaccaccagt actcaccacc gactaatgag gctaacaaaa cgcccagcca ggggagcata
    67321 agatcagcca accaacccgc tcccatctgg tgaaattgat aatctgccag atgcagcgcg
    67381 attatccaga cccccagggt taataatgcg ggtactaaaa aaagacattg tcgccagacg
    67441 gctatttctc ttttttctgg tgcactatcg tccgcccaca ctcgccccga gaaaaatagc
    67501 attaatgcgc ccagcgccag cataaaaatg ttgagcagaa ggtaaattgt tggcgtgata
    67561 aagatattac aaacttcaat aataccggca agtccgacac ccagcatggc aataaccgcc
    67621 gccaaaaact gcgccagaat ggggatgccg aaaaaagtca ttaccagcga ggtcaaaatc
    67681 cattttctgt tttgcattat tctttccatt ctttctgaat ggcgaaatta tactccccga
    67741 gtccccttgc cccttctgga cacttttccg aaatgatggc gaaaaaaacg ggaccctttg
    67801 gccccgttct atttattagt gaaattacaa tctcaccgga tcaatatgcc agatatgatc
    67861 ggcgtactct ttgatagtac ggtcagaaga gaagtagccc atattggcaa tgttcagcat
    67921 cgcttttgcg gtccactctt cctgaagctc gtagagttca tcgactttat cctgacaatc
    67981 gacatagctg cgataatccg ccagtacctg gtagtgatcg ccgaagttga tcagcgaatc
    68041 aaccagatcg cgatagcgac ccggatcttc cggactgaat acaccgctgc cgatttgcgt
    68101 cagcacctga tgcagctcct catctttctc gtagtattca cgcggtttgt agccctgacg
    68161 acgcagttct tccacttctt ccgctgtgtt accaaagata aagatattgt cagcaccgac
    68221 atgatccagc atctcgacat tcgcaccgtc caacgtaccg atagtcagcg caccgttaag
    68281 cgcaaacttc atgttactgg tgccggaagc ttccgtcccc gccagcgaaa tctgttcaga
    68341 cagatcggcc gccggaatga tcaactgcgc caggctaacg ctgtagttcg ggatgaacac
    68401 gactttcagc ttgtcgccaa tctgcggatc gttgttgatc actttcgcta cgtcattgat
    68461 caaatgaata atgtgcttcg ccatgtaata ggccgaagcc gccttaccgc caaaaatatt
    68521 cacgcgcggt acccacttcg catccgggtc ggccttgatg cggttatagc gggtaatcac
    68581 atgcaacaca ttcatcaatt gacgtttgta ttcgtgaata cgtttgattt gtacatcgaa
    68641 caacgccttt ggattcacca ccacattcag ctgctgggcg atatactctg ccagacgctt
    68701 tttgttctcc agcttcgcct gatgcacagc gtgattaacc attgggaaat cacagtgttg
    68761 ttgcagctca ttaagcaggc taaggtcggt gcgccagtta cggcccaggt gttcgtccag
    68821 cacggctgaa agcgatgggt tcgctaccgc tagccagcga cgcggcgtca caccgttggt
    68881 gacgttggtg aaacgacccg ggaagatttt cgcaaagtcg gcaaacaacg attgcaccat
    68941 cagattagag tgcagttccg atacaccgtt aactttgtgg ctcacaacaa ccgccagcca
    69001 ggccatacgc acacgacgac cgttggattc atcaatgatc gacgcccgtc ccagcagatc
    69061 ggtatcgttc ggatactgtt cctgcaaggt tttcaggaaa tagtcgttga tttcaaagat
    69121 gatctgcagg tgacgcggca gaattttacc cagcatatca accggccagg tttccagcgc
    69181 ctcgctcatc agcgtgtggt tagtgtagga gaagacctga caacacacct caaacgcgtc
    69241 gtcccagcta aattggtgct catcgatcag cagacgcatc atctcaggaa tcgacagtac
    69301 cggatgggta tcattgagat gaatcgcgat tttatccgcc aggttatcgt aggttttatg
    69361 caactgataa tggcggctta aaatgtcctg aatggtcgag gaaaccagga agtattcctg
    69421 acgcaggcgc agctcacgcc cggagtaggt ggagtcatcc ggatacagta cgcgagatac
    69481 gttctcggag tggtttttat cttccactgc cgcgaagtag tcaccctggt tgaatttacc
    69541 gaggttaatt tcgctactgg cttgggcact ccacaaacgc aacgtgttgg tcgcgtcggt
    69601 atcgtaacca gggattatct gatcgtaagc gactcccaga atctcttcgg tttcaatcca
    69661 gcgcgttttt ttaccttcct gctgaatgcg accaccaaaa cggactttat agcgcgtgtt
    69721 gtggcgtttg aattcccacg ggttaccgta ttccagccag tagtctggcg actctttctg
    69781 gctaccgtta acgatgttct gcttgaacat accgtagtca tagcggatgc cgtaaccgcg
    69841 ccccggcaac cctaacgtcg ccagagaatc aaggaagcaa gccgccagac gtcccaggcc
    69901 accgttaccg aggcctgggt cattttcttc atcaatcagc tcttcgagat ttaaccccat
    69961 cgcttccagt gcgccctgta catcttcgta aattcctagc gacaacatgg cgttggagag
    70021 cgtacggcca atcaaaaact ccatcgacag gtagtaaacc tgacgagttt cttgcgacaa
    70081 ctgggcacgg tttgaacgta accagcgctc cacgagacga tcgcgcacag caaataacgt
    70141 tgcgttcagc cattcatgtt tattggcgac gaccgggtcc tttccaatcg taaacatcag
    70201 cttgtaagcg atagagtgct taagagcttc tacgctaagc gtgggcgatg aatatgtaaa
    70261 cggagcattc atataggcgt ttcctgaaaa ctatttcaag cgatagtaaa gctcacggta
    70321 cgacttcgcc gcgacctgcc agctaaaatc cattgccata gcctgacgtt gcacaaaccg
    70381 ccacagtgaa ggacgggacc acagtacaaa agcacgccga atagcccgta acagcgacca
    70441 ggcattacta tcttcaaaga caaacccact ggcgacgcca tctgcaaggt tctcaagaga
    70501 acagtcagaa accgtatcag caagcccacc ggtgcgccgc actaacggca gcgtaccgta
    70561 cttcaatcca taaagttgcg ttaagccgca cggttcaaaa cggctgggca ccagaatgac
    70621 gtccgcgccg cccataatgc gatgcgaaaa tgcttcgtga tagccaatct gaacgcccac
    70681 ctgaccgggg tattccgctg ccgccgcaag gaaaccttcc tgcagcaccg gatcgcccgc
    70741 gccgagtagc gccagctgcc cgccctgctc cagaagaccc ggtaaggctt ccagcaccag
    70801 gtcgagacct ttctggctgg tcagacggct caccactgca aaaagcggca ctttatcgtc
    70861 aaccttaagc cccattgcga tttgtaactg gcgcttattt tccgctttat cttccaacgt
    70921 atcgcgggtg taacgcgagg ccaacagtaa gtccgtctct ggactccaga ttttctcgtc
    70981 cacgccgttc agtacgccgg aaagacgccc ttcacggtga cgctgttgca acagaccttc
    71041 cataccgtag gcaaactgcg gttcggtgat ctcgcgagcg taggttggac tgaccgccgt
    71101 aatgtgatcg gcatagtaca gaccggcctt caggaaagag atttgtccgt tgaattccag
    71161 cccatgaata ttaaagaatg accatggcaa ttggatgtca ttcatgtgat gtgcataaaa
    71221 catgccttga taggccaagt tgtgcacagt aaacaccgac ttcgccggac gcccgcgcgc
    71281 cgccagatac gcaggcgcaa ggcctgcatg ccagtcgtgc gcatgcacca catcaggacg
    71341 ccagaatggg tcaagcccgc tggccatttc tgccccaacc caccccagca gcgcaaaacg
    71401 caatacgttg tcggtatagg caaataagtt ggtatcgtga tacgggcttc ccggacgatc
    71461 atagagatgc ggcgcgtcaa tcaggtatat gccaaccccg ttgtaatgac cgaacaacag
    71521 cgtgatatgt ccggcgaagg tatcacgacg ggatactacc tgcgcatcgg tcacgccacg
    71581 gcgaatatcg ggaaatgcag gcaacagtac gcgagcgtca acgccgtctg cgatttgtgc
    71641 tgcgggtaat gccccaataa catcagccag accgccggtt ttaagcagcg ggaacatctc
    71701 tgaacataca tgtaaaacct gcattatcgc tcctgtttat gccctaactt ccgtagcatt
    71761 tcgcgcgtta ccagcacgat gccttcttct gaacgataga aacgacgtgc atcttcctct
    71821 gcgttttcac caatcaccat gccttccgga ataacacaag cacgatcgat gacgcagcgg
    71881 cgcagacggc acgagcgacc tacccatact tccggtaaca atacggcgga atcaatgttg
    71941 cagaatgaat tcacgcgaac gcgcgagaac agaacggact gcaccaccac cgaaccggag
    72001 atcacacaac cgccggatac cagtgagtta agggtcatcc cgtggctacc ggagcgatcc
    72061 tgcacgaatt tcgctggcgg taatgattca ttgtaggtgc gaattggcca attgcgatcg
    72121 tacatatcca gttccggcac cacagaggcc agatcgagat tcgctttcca gtaagcttcc
    72181 agcgtaccca catcgcgcca gtacggctcg gcatccgggt cggattgtac gcaagagagc
    72241 gggaacgggt gcgcataggc cagaccggct tcggtgatct tgggaatcaa atctttgcca
    72301 aagtcgtggc tggagttctc atcacgatcg tcttcttcca gcagttcata cagatagtcg
    72361 gcgtcaaaga cgtagatacc catactcgcc agagatttgc tcggatcgtt cggcattgac
    72421 ggtgggttag caggtttttc aacgaattcg ataattttat cgttctcatc aaccgccata
    72481 acgccaaatg cggaggcttc ttcaatcggt actggcatac aagcaacggt gcaacgtgcg
    72541 cctttttcga cgtgatcgat aagcatacgc gagtagtctt gcttgtagat atggtcgccc
    72601 gccaggatca ccacgtattc cgctttataa cggcggataa tgtcgaggtt ttgggtgacc
    72661 gcatctgcgg tgccgcgata ccagttttcc cctttcattc tctgctgtgc tggcagcaga
    72721 tcgacaaact cgttcatttc ttcattgaag aatgaccagc cgcgctgaat gtgctgcacc
    72781 agagtgtggg actggtactg ggtgatcacg cccatacgac ggatcccgga gttgatgcag
    72841 ttagacagcg caaagtcgat aatgcggaac ttaccgccga agtgtacggc cggttttgct
    72901 cgcttattgg ttaaatcctt caggcgggta ccacgtcctc ccgccagtat cagggcaaca
    72961 gatttcaatg gcagctggcg cgccaacatt aagtgatcgt tcttctctaa actaaccatg
    73021 actaactcct tttttatcat ctctggaaca cacacaatcc gtgtgcaggt ccctgccaga
    73081 cagccgtaat cactgggtta tcctctccag cgaatggggg aatggcgtgc cactccccag
    73141 caggtaaaac aatctctgtt acctcaagcg tggcgttaat tgcgatcaaa aagcgatccg
    73201 agagcagaat ttgcagctgt ttcggcccgt tttgccactc atccgtgctt aaaggttgag
    73261 catatcgatt tagccaacgg acattgccgt cgccttcttc ccaccagcga ttctccacca
    73321 aagcgggaat gcgcttgcgc agatggatta acgcggcggt aaatgcggtt aaaccactgc
    73381 ttgcctgcga ccagtccaac caggttaatt ggttatcctg acagtaggca ttgttattgc
    73441 catgctggct gtgaccatgt tcgtcaccgg ccagtaacat cggcgtaccc tgggagagca
    73501 acaacgttgt taacagggcg tgaatgctgt cgcgccgccg ttcaaccagg tcaagagaac
    73561 cgcctaaccc ttctttacca tgattgttac tgtaattgtt gttggtcccg tcgcgatttt
    73621 cttctccgtt tgcttcattg tgtttatggt tgaagcaaac gcagtcgcga agcgtaaaac
    73681 cgtcatgcgc ggtgacgaga ttaatcgcgg cactcggcag acgaccatta cgtttaaaaa
    73741 catcgctgga ggcagcaaaa cgcccggcaa acgcccccag aggcaaatca taatgtagcc
    73801 agaaacgacg ggcagcatcg cggaaatgat cgttccactc ggcaaacagc ggcgggaaat
    73861 ttcccacctg ataaccacca ggagcgatat cccacggttc agcaattaac ttcacctgcg
    73921 agagcaccgg gcagttctgg atagcggtaa acaacggcgc atcctgacgg aactctggcg
    73981 tacggcccat gactgccgcc agatcaaagc ggaaaccatc gacgtggcag gtttctaccc
    74041 aataacgcag gcaggcgctg gcataatcca ccaccgccgg atgactcaaa ttgagcgtgt
    74101 tgccgcaacc ggtccagttg tgataatcgc cgtcttctct tatccaataa tagctacggt
    74161 tatcgatccc acgcagcgag aataacgggc cgtcgaggtc cagttccgca ctatggttga
    74221 gcacgatatc aagaatgact tcgatacccg ctttatgcag tgctttgatt gcatcgcgaa
    74281 actcatccag cgccgtttct ggcgagcagg catacgccgg atgcagcgca aacatcgcca
    74341 ccgggttgta accccagtag ttacttagcc ccatgcgttg cagacgtggt tcactggcaa
    74401 actgcgccac tggcagcagt tccagcgcgg taatgcccaa ttgtttcaaa tagttgatca
    74461 tcaccggatg cccgagggct ttataagtgc cacggatctc gaccgggatc tccgggtgca
    74521 agtacgttaa tcctttgaca tgggcttcat aaatgatggt gctgccccac ggcgtgcgcg
    74581 gcggggcatc atcttcccag tcatagtgat caaccaccac tacgcatttc ggcgcaatgg
    74641 cggcgttgtc gcgatagtca ggttcattat gaccggcgtg cagcagcggg ttatctttaa
    74701 actccccgtc aatttgccgc gcgcaaggat caatcaacaa cttcgccggg ttaaagcgat
    74761 gcccctcggc gggttgccag gggccatgaa cgcgataacc ataacgcaaa cccgggcgcg
    74821 catccggcag ataaccgtgc caaatgtcgc cactgtgccc tggcaagtca tagcgatgtt
    74881 cctggccatt ggcgtcaaag acacacagtt ctacccgctc ggcatgagcg gagaaaagtg
    74941 tgaagttgac gccctgaccg tcgtaatgcg cgccgagggg agcgggtttg ccaatggcga
    75001 gttgtgtcat tctgcctccc gaaccagcca gatagtggcc agcggtggta gcgtcaggct
    75061 tagtgaatgc tgacgaccgt ggctggcaat ctcatcgctg tgtaccgtgc cgccattgcc
    75121 tgcattactg ccgtgatagt gcatggaatc ggtattgagg atttcacgcc atttgcccgg
    75181 ctggtttatg ccgaagcgat aatcatgacg cggtaccggc gtaaagttac tggcaacgat
    75241 gatttcgtta ccctctttat cgcgacgcac aaagatcagc accgagcgtt ctttgtcatc
    75301 caccaccagc cattcaaagc cgtacgggtc aaaatccagt tcatgcattg ctttatggtg
    75361 gcggtaggtg aggttcagat cgcgcaccag acgctggaca ccgtggtgcc agttatcgcc
    75421 gccttccaac agatgccagt cgaggctggc gtcatggttc cactcgcggc cctgggcaaa
    75481 ttcgttaccc atgaacagta gtttcttgcc cgggaatgcc cacatccagc catagtaggc
    75541 gcgcaggttc gcgaatttct gccatgcgtc gcccggcatg cggtcgagaa tcgatttttt
    75601 accgtggacc acttcatcat gcgacaacgg caggacgaag ttttcagtgt agttgtagag
    75661 aatcccgaag gtcagtttat cgtgatgata ctgacgataa accgggtcga gcttcatgta
    75721 gtccagggtg tcatgcatcc agccgaggtt ccacttgtac cagaagccca gaccgcccat
    75781 atcctgcgga cgagaaacgc cagggaaatc ggtagactcc tcagccattg tcaccgcacc
    75841 ggaaacctgc tcaccaagaa tacggttggt attacgcaag aattcaatcg cttcaagatt
    75901 ctcgcgcccg ccaaattcgt tcgggatcca ctccccctct ttacggctgt agtcgcgata
    75961 aatcattgac gccaccgcat cgacgcgcag cgcatcaata ccaaaacgtt caatccagta
    76021 aagcgcgtta ccgacgagga agttactgac ttcacggcga ccatagttgt agatcagcgt
    76081 gttccagtcc tgatgatagc cttcacgcgg atcgctgtgt tcatacaagt tcgtgccatc
    76141 aaattcggca agcgcaaagt catcagtcgg gaagtggcct ggcacccagt cgagaatcac
    76201 gttcagacca gctgcgtgtg cggcatcaat gaaataacgg aagtcgtcgc gagtaccaaa
    76261 acggcgggtt ggtgcataca ggccggttgg ctgataaccc caactgccat cgaagggatg
    76321 ctcgttaatg ggcagtagtt cgaggtgggt aaagcccatc catttagcat aaggcaccag
    76381 ttgatcggcc agctcgcggt agctcaacca gaaattgttg tcggtgtggc gacgccagga
    76441 acccaggtga acttcataaa tagagattgg cgcatcaaac tgattcgctt ttttgcgctc
    76501 ttcagtctgt acaacctttt ccggcagccc gcaaataaga gacgcggttt ccgggcgcat
    76561 ttgcgcttcg aaggcataag ggtcggactt cagacgcaag ttgccattgg catcaatcat
    76621 ctcgtattta tagagctgac cgttatgcgc cccagggata aacagttccc agatgccgct
    76681 ctctttacgc aggcgcatcg ggtgacggcg accgtcccag tagttaaatt gcccaaccac
    76741 cgagacccga cgggcgtttg gagcccagac agagaaacgc gtacctgtga cgccatccat
    76801 agtatctgca tgcgcgccta aggtttcata cgggcgcagg tgagtacctt cagataatag
    76861 ccaggcatcc atttcctgga ttagcggacc aaaacggtaa ggatcatcaa tcaggttttg
    76921 ctgaccatgc cagacaacag ccaactgata gcggaaaaaa ttcttacgtc gcggaatgac
    76981 gccgctaaag aatccccgtg agtcgagaca ctccagtttt gcgagtttgc gcccggtttt
    77041 cggttcaatc acccacacat cggtagcgtc gggtaaaagg gcacggactt ccagtcccgc
    77101 ggtggtttta tgcattccca gtacggaaaa aggatccgca aaatggcctg caattagcgc
    77161 gttaatcacg tctctatcga tacgatcgga catgcttgtc ttcctgtttt attgtgtcac
    77221 cccatccaat tggatttttg acttctggtt gtgacatttt tttgacctga acggcgcagc
    77281 actctgtgca tcctctctgc gtcgtcctca cttcaggtaa ggctgtgaat actcatgtat
    77341 tcagccaccc ttaaagaata gccaatgctc tatttaactc ccggtaaatc atgaaacatc
    77401 tgcgcttact cctgtattac gcactaacag gggcggcatc gcgccccaga tttaatgaat
    77461 aaagattacg ccagttgacg aagcatccga cgcagcggct ccgcggcccc ccacagcagc
    77521 tggtcgccca cggtaaaggc tgacaggaac tctggtccca tattcagctt acgcaggcgg
    77581 cctaccggcg tggtcagcgt gccggtaacg gcagctgggg ttagctcacg catagtgatt
    77641 tcccgatcgt tcggaacgac tttcgcccac ggattgtgcg cagccagcag ttcttccacg
    77701 gtcggaatag acacatcttt tttcaattta atagtgaatg cctggctgtg gcagcgcaat
    77761 gccccgacac gcacacataa accatctacc ggaattacgg aagatgtgtt gaggatcttg
    77821 ttggtttccg cctgcccttt ccactcttcg cggctctgac cgttatcgag ctgtttgtcg
    77881 atccacggaa tcaggctacc cgccagcggc acgccaaagt tatccaccgg cagctcaccg
    77941 ctacgggtta aggttgtgac tttgcgttcg atatcgagaa tagcagagga cggggtcgcg
    78001 agttcatctg ccacatggcc atacagatgg cccatctggg ttaataactc acgcatatgt
    78061 cgcgcaccac cgccggaagc ggcctggtag gttgcaacgg acacccaatc aacaagatca
    78121 ttggcgaata aaccacccaa cgacatcaac atcaggctta cggtacagtt accgccaaca
    78181 aaagtcctga tgccattatt taatccgtcg gtaatgacgt cctgattgac ggggtcaaga
    78241 atgatgatgg cgtcatcttt catgcgcaga gacgatgctg cgtcaatcca gtaaccttgc
    78301 catccgcttt cacgaagctt tggatagatt tcgttggtat aatcgccgcc ctgacaggtc
    78361 acaatgatat cgagggcctt tagcgcctcc agatcaaagg catcctgaag tgtgccagtg
    78421 gttccgccaa aagacggcgc agcctggcca agctgagaag tagaaaagaa gacagggcga
    78481 atggcgtcga agtcgcgctc ttcaaccatg cgttgcatga gaacggagcc gaccataccg
    78541 cgccagccga taaaaccaac atttttcata agcgtttttt tcctgcaaag atgtgtgctg
    78601 tataaatgtg ccggtctcct cttggcacat ctttcaccat acaaaaagca gccaaagtcg
    78661 caagtgaaat taatcaatga tagcgaagcc atcagtaatg cgacttatcc tgctttgtta
    78721 gcacgcagaa agtccgcggc aattatcagg gaatttgagt tatgaatgaa atcatttctg
    78781 cagcagtttt attgatcctg attatggatc cgctcggaaa cctacctatt ttcatgtccg
    78841 tactgaaaca tactgaaccg aaaagacggc gggcaatcat ggtgcgagag ttgcttattg
    78901 ctctcctggt gatgctggtg ttcctgtttg cgggtgagaa aattctggca tttctcagcc
    78961 tgcgagcaga aaccgtctcc atttctggcg gcatcattct gtttctaatc gccattaaga
    79021 tgattttccc cagcgcttca ggaaatagca gcgggcttcc ggcaggtgaa gagccattta
    79081 tcgtgccgtt ggcaattccg ttagtcgccg ggccgactat tctcgccacg ctgatgttgc
    79141 tgtctcacca gtacccaaat cagatgggac atctggtgat tgctctgctg ctggcctggg
    79201 gcggcacctt tgtcatcctg ctacagtctt cgctattttt acgtctgctg ggcgagaaag
    79261 gggtgaacgc acttgaacgc ctgatgggat tgattctggt gatgatggca acccagatgt
    79321 tcctcgacgg cattagaatg tggatgaagg ggtaaggttc aaacaggtgc tgcacacaac
    79381 cagacgcggc acctgtttta ttacctgaac tgataatgac cttcgcccaa tcggataacc
    79441 cttccctctt cttctgcgcg gttcagccac acactaattt gttcgatggt taaaccagta
    79501 caagacgaca gcctttcaat acttatcgat tcctttgcaa agatcgccag ttccgccaca
    79561 aagagttgat aaaaatcgac aggcgtatgc gcgggcatta ctgcgtaatt tgcgtcattt
    79621 tcatgcagcg aaaacagttc tggttgagcg ttacggagtg ctctgtcata catcagccca
    79681 ggaacgtctg acagtctgat cggcacattc tcagcctgtt cagcactcca tgacgcccca
    79741 ttttctacta atcgtgaatt ggctgattgc atatcctgat ttggtttaac ctgcacaggc
    79801 agccactgat gttttaatgc ctctatcgcc ccggttatcg ttccaccggt cattcccgca
    79861 cgtacaacca ttgcgctttc agcaaggcaa taaatatatt tatttcgcgc catcgcattg
    79921 ccgacggtga aacgcacctc tgggtaaaac ggcgaaatca acaccaggtt gcctgctata
    79981 agcccttcac gccatttcac taacgtactc gtttttaaca agctatcagc taatacgcca
    80041 acggcagttc ccccggcctc cagtgccgac gccattgcac attcatcaat acctcgcgca
    80101 ccaccagaga taacgcaaat cccctgttgg gccagtttcg cggccagttg ttgggtatag
    80161 cgcaaatcgt cagtcggagc atctcgcgag ccaacaatcg ccataccttc cgcttgcagt
    80221 aatgctttat tcccgcagcc aaataaaacg ggaggtgcat ccgttcgcaa tcggtttttc
    80281 aagcgaacgg gataatcagc atctcctcgg gttatgatcc ataaaccggc gcgttgccac
    80341 ttatctaccg ccaacgccag actatgaccg cgcgccagca agccaagaat acgctcctga
    80401 gaaatacgcg gatcgctcca gcctgtaagt ttctcttgcg gttgcggcac cagaagctcg
    80461 gcgggactga tacgttggtg cttcagccat aatgcaaact tcccccattc actattacta
    80521 agaggtttat gctcactcgc cgccgcgcga gaaaaatcgc tggttagcag gagagtcgct
    80581 tgtgcattgg ctgaaagatt catcaatttt ttaccgaggt agaagcaagg gcaagaggat
    80641 aaaccgtcgg gcaacctgcc tggcgtaaca gtgctgtcag aactgtcagc gtccatgcag
    80701 aatccacgat atcgtcaacc agcaacgccg gacctggcat caaaggaggg gtaatcacaa
    80761 acgccccgtc gagattttga cactggtgaa aacggttttg ctgcattttc tgcggtggat
    80821 tgtccacgac tttttcaatg gcatcaataa aaggtaagcc aagtttcgcc gccagtcgcc
    80881 gggcaaaatc aggaaccagg tcgaggtgcc ttaatgaagg aacgcagcat acccaggttg
    80941 gttcaggatg cggattccag cgttggcgaa ccatttccgc acatgcctcc accagctcat
    81001 cactgaatcg ccccgttttt ttctcctgtg ctacctgctt tccccatccg gaatctcccc
    81061 aacgagaaag gactcgcccc gtagatcctt gtaaacccgc aggcaagttc cctttaaatc
    81121 cgtattgcgt aaaagctcca gatgctacct gtttattgag atttaatggc aggtcagcgt
    81181 gtttaataaa taaatttgcc gcatggagta agccgctatc aatatcgggc gacaacaacg
    81241 gatgctgaag acaactgcta catttaccac aacgttcggc acttggctca tcgagtgcgc
    81301 ggcgcagaaa ttgcattttg cactccttgg tcgtgatata gctttcaaca tctgccagct
    81361 cattttttct ctgattcatc aaatggttaa ttcgttcatg gtcaggagag aaactgacga
    81421 tagttctgcg ccataatttc tcggtataca ccacaggcga tggattttca gctaccaata
    81481 atttcagtgc tttttctatt tgcccataac ggagattcgt ccgttgttca atgcctcgta
    81541 gggtaagacc gtcattctcg ctaagtacgt tgagtatttc atgaatttgc gcctccgcag
    81601 gaaaggcgct ttcacggaag aatttatgaa tagcgcggtc ttcaccacca caaagcaata
    81661 tgccaacagc ggaatctata gcacgcccgg cacgccctac ctgttggtag taaccgacaa
    81721 tagaccccgg catctggtaa tgaataacaa accccaaatc cggtttgtca aagcccattc
    81781 ccagcgccgt cgtcgcgacc agaactttaa ttttatttgc cagcaacgcc tgctccagat
    81841 attccctggc agtattagaa tcttccgcgc cttcacaagt cacgccgctg tagtaagcaa
    81901 atgcactgat accatttgtc cttaaccact cggcgacaag gtcggcatcg cgagtagtca
    81961 gggtataaac aattcccgat ttggaaaact gagggataac cgttgctaac catgccagac
    82021 gggatgactg ttcacctaag actaaggcat caagcgccaa actttcgcga gccagcgttc
    82081 cacgctgaat cacaatgtca cccaattgct gacggatatc ctcaacgaca cggttattcg
    82141 ctgtcgcggt tgtacccaga actggggtat tcgcaggcag ttggcgcaaa atatctaata
    82201 tacgtcggta atccgggcga aaatcgtggc cccaatcaga gatgcagtgc gcctcgtcga
    82261 ccacaagcaa gccaatacga tcggctatag gatataaaac cgtttcgata aagtcttggt
    82321 ttgccaaacg ttccggggaa accagcaagc aatcgacctc accttgcagc aacttatcgc
    82381 taatacgctg ccattcttcc ctgtttgtag aattcaacgt ttcagccgta atacctaacc
    82441 gttcagcggc ggcaacctgg ttacgcatca gggcaagcaa aggagagatg ataattgtcg
    82501 ggccagcacc gcgatcgcgg aagattttac tggcgatgaa gtacacggca cttttccccc
    82561 agccagtgcg ttggacaacc agcagttttc gtcgttggtt caccaccgcg tctatggctt
    82621 cccattgccc ttccctgaaa gtagcggtgg tatttcctaa catccgctgt aaaagcaatt
    82681 cagctgcgtg cttttccatc tttatctcct gcgatcttta ggcagtgatt agaacggatt
    82741 attagcaggt aaatttgcga gagggatcaa aaagatgaag agaggagata agagaaatga
    82801 attactgcga gcaagctcgc agtaatttgt cgcagtaatt aactcaacag ctggaacgca
    82861 atcatcccaa cgatggcacc gacagtgccg aggatggttt ccatcatggt ccaggttttc
    82921 agcgtttcgg cttcggtcgc gccggtaaat ttaccgaaca accagaaacc ggcgtcgtta
    82981 acgtggctga caacaatcga accaccagcg atacaaatcg acagcgccgc catttgcgca
    83041 ccggagtagt tcagttgttc aataatcggc atcaccagtc ctaccgccgt taaacaggct
    83101 acggtggcag aaccctgaat gatgcgcact gcagctgcca gcacgaagca ggtgatagca
    83161 atcggcaggc ccatgccggt taacgcttcg cccagtgccg gacctacgcc agagtcaacc
    83221 agcacctgtt tgaacacgcc gcctgcacca atcaccagca gaatgatccc cgccggttgc
    83281 agcgcgtgac cgcaaatctc catcactttg tcttttggca tgccctgacg cattgccagg
    83341 ccataaatcg ctaccagaca agcaaccaga atcgcggtaa acggatgacc aataaactcg
    83401 aaccattcgt aagcggtaga gccttctggc acaaaacgcg cggcaatggt tttcagcccc
    83461 accagcacca gcggcaacag gatcagcgac aggctgaatc cgaaagatgg cattttgcct
    83521 tcgccgagat gcggttcgct gatgtcgtca ggaatatgca actccacgta gcggctgatg
    83581 aaattacccc acagcggccc ggcaataatc attcccggaa ttgccgcaca caggccaatc
    83641 aggatcatcc agccaaaatc ggcgttcatc tgcgatgcca gcagcattgg cgctggccct
    83701 ggcaccagga acgccgccgc tgccgccacg cctgcgaata atgggattac cagcttcacc
    83761 aggttcgtac cggtgtggcg cgccattgag aaagcaacgc taatcagcag aacaatcgcc
    83821 acttcaaaga acagcggtag cgcacagacc agccccgcaa ggccgatggc ataatgcgcg
    83881 cggctgtgac cgaaggattt aagcattttg acggcaatct gatcgactgc gccggtttca
    83941 tgtaagatct tgccaaacat agctcccagg gcgacaacca ccgccaggaa gccgagggtg
    84001 cctcccatcc ctttttccat cgtcgctgcg attttatcga gcggcatacc ggaaaaaagg
    84061 ccagccccca tggacaccac catcaaagcc aggaaagcgt gcatacgcgc cttcatgact
    84121 aaaaacagca gcagtaaaac agaccctact gctgttaaaa caagcgttaa tgtagtcact
    84181 acttatttgc cttttttaat aacctcaatg gtgcttgcca caacaccttc cagcggttga
    84241 tcgatatcca ccaccagtac atcagtttcg tccgcacccg gctcctgcag cgtttcaaac
    84301 tgcgtcacca acatttgggt tttaaagaaa tggcctttgc gcgctttcag gcggctttca
    84361 atcacgtcaa aatcgccttt caggtagatg aaagagagat tcggattacc ttcacgcagc
    84421 aagtcgcgat agtgtttttt caatgcagaa cagacgatca gtgacacttt attagtgcgc
    84481 tgcatagcaa acgcggcgtc gttcagcgcc tgcaaccacg gtttgcgatc gtcgtcattc
    84541 agcggttcgc cagacgccat tttttcgata ttgcgccgtg gatggaggaa atcgccatca
    84601 agaaacgcgg catgaagttg atgcgccact tcactggcga ccgcagattt gccgctgcct
    84661 gagacgccca tcaagacgta aatgtggtga tcatggttag tcgtgctcaa agtggtgccc
    84721 ccacaataca agaattaaaa atgttacggg taactgttat cggtaacatt gtccggccgg
    84781 acaatatcag aagcaatatc catccgtgcc ttaagtgtat aagtgtgagc tacttcaaat
    84841 ttgtgggctt aaatagatcc gcccggtgac aaggtgaaac ctaaatctaa cattttcggt
    84901 gtcacagatt cgccacgaat acgcgccagc aggcgttcag cgccaatact gcccatccgc
    84961 tcacgcggcg tcagcacgct cgccagtcgt ggctccatca cctgaccaat gtcatgaccg
    85021 tggaaaccgg caatcgccat atcgtcagga acttttaacc ccagacgctg acattcaaac
    85081 gccgcgccga ccgccagatc atcattagta cagaacacgc catccagctg cggatattcc
    85141 cgccgcgcct ggcgaatcag ttcaataccg gaagagtaag aagaagattg ctcaaccatc
    85201 acgctatacg gcaccaggcc tgcatccagc atcgcctgtt cgtatccctt ctgtttgatg
    85261 atagtacgtt cgtcgagacg tgcgccgaga taggcaatgt ggcgatgccc gcgcgcaata
    85321 atggcggtag tcatctggcg tgccgcttca aagttatcaa aaccgacggc gatatcgagg
    85381 cacggcgact ggctgtccat caactccacc accgggatcc ccgccacttc aatcatcttt
    85441 aaggtgcgcg gcgtgtgggt acgttcggtg aggatcaggc cgtcgatatt ccaggacagc
    85501 atggattcga gacgttcttg ctccatttcc ggtttataac cgtagtgcgc cagcatcgtc
    85561 tgataaccgt gcgcgtcggt gacgctttcg attccgcgta atacttccgc gaaaacctgg
    85621 ttggtgagag aaggtaacag gacgccaatc gcccggctgg tagcgttaga gaggatatcg
    85681 ggcgcacgat tgggaatata gcccagttca tcaagagcgg cggcaatctt gccgcgtaga
    85741 gcgacggaaa cctgctccgg gttacgcaaa aagcggctga ccgtcatttt ggtcacgcct
    85801 acacggtcag ccacatcctg aagtacgggt cttttctttt tcatcgtcct gaaggtacaa
    85861 aagagataga ttctcttagt ttaacacgga cgcatcagaa ccttcccggg gaaaacagtg
    85921 cttgtttaca attattagat atacactaaa tcaattgcat cgcattgtgc taacgacgcg
    85981 gcaaaacccc ggaagcgtgc atcagcacgc gaccggagcg agcgaggaag cctgcaaaaa
    86041 ggcaggcttc aaagatgccg ttgagtttta aaccggcggc agatcgaaca gtaacacttc
    86101 gctgtcgcta tccgcatgga tggagattgc ctgctcatcc cagattgcca gaccatcgct
    86161 ggtcgaggct ttcacgccgt taatggtgac attgcctttc accacctgga tccagacgcg
    86221 gcgttcagcg gcaatctgat gcaccgactg ctcatctttc agcaacgccc aacgatacag
    86281 ttccatatcc tgatgcactt tcagcgaacc atctcgcgca tccggcgaga gcaccagctg
    86341 tttgccctgc acggcatcga agcgacgctg ttcataacgc ggcgtaatgc cattttcttc
    86401 aggcatgatc cagatctgat acagatgcag acgctcggtg ctgcttgggt tgtactctga
    86461 gtgacgaata cccgtacccg cactcataat ctggaactca cccgccggaa cctgctcttt
    86521 attgcccatg ctgtcctgat gctcaacagt accttccagc acgtaggtca aaatttccat
    86581 atctttatgc gggtgagtgc cgaagccctg ccctgcttca atcacgtcgt cgttaatcac
    86641 gcgcagcgcg gaaaagccca taaagttcgg atcgtaatag ttggcaaaag agaaagtatg
    86701 ccaggagtcc agccagccat gatttgcatg accacgttca tttgctttgc gtaagtagat
    86761 catttgtatt cgccccatga atgatttcga tgggcttagt gtggacccga tctgcctggg
    86821 atgatagagg gtgaaaattg acccctctgt tcaaaaaaat tgaactattc gaggggccat
    86881 caaacttact tagcgagagt tactgtggag ggagaggctt gctcaaatcc gcgttcaagg
    86941 atttccagat tcgtaagaac ttcagattcc ttgacgtaat ttggcgcacc gtgggtgatg
    87001 gtttgataca acgcatcata aacgcgcccg taatcgccca tctccggctt catctcttct
    87061 ctgaccgtca cgccctcgtc attgacatac tccagcacac cgaccgaatc atccgctgcg
    87121 aatcccggtt cgcccggcat aatattagcc ttcaggctgg tttcctgctg gtcgataccg
    87181 tatttaataa acgaaccttt cttaccgtga acgataaatt tcggataatc gattttcacc
    87241 agatggctgg ttttgacgat ggctttcagg tcgccataaa acagttgcgc ttcaaaggtg
    87301 tcgtcaggat tggctttatt acgcaggctg cggatgtcat aagcgacgtg atccgggcga
    87361 ccgaacagag aaataatctg gtccatcgta tgcacaccaa ggccataaaa cgcgccatcc
    87421 tgcggcagcc caggtttggt ctctgccacc gggcggtaat agtcaaaatg gctttccact
    87481 tcaacaatct ctcccaactt gccactttca atcgcttttt tcgctgtcag gaagcaggag
    87541 tcaaagcgac gattctgata cggcgtgacg gtcagccctt tgcttttcgc caacgcaaac
    87601 agctccttcg cctgggcaag cgtcggggtg aacggttttt cgaccagcac atttttcccg
    87661 gcttccagcg cgcgtttcgc gtactcgaaa tggctgtccg cgtgggtgca gacaacaacc
    87721 agcttaacat cgggatcgtt tagtacttcg tcgagatcgc tggtgaaatg gatatgggaa
    87781 taaatgggag cctgttcttc cggcttcgca tggcgacgaa aaatatgcgc gacatgccag
    87841 ctatccttgc ggttaagtac atacggcaga tggtaacggg tggtgctttt gccgaagcca
    87901 ataaaggcgc agttgatgac catgatttcg tcctttttaa ggtggttatt tacaccttag
    87961 cgcaaagcgg acgtggttcc tactggagtg cgcataacga acacaagcac tcccgtggat
    88021 aaattgagaa cgaaagatca aaaaaaagcc agcacccggc tggctaagta atactggaag
    88081 caatgtgagc aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgagacaa
    88141 taataatcat tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg
    88201 catttgaaag tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt
    88261 gagatagtaa tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc
    88321 cagccggagg tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag
    88381 gagcgactcg ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc
    88441 gtgggccatc tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt
    88501 ggtatctgtg tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag
    88561 atgattgaaa tgtgcgacaa ctggttgcgg gtagatcgca ttgaactaac cgtgtttgtc
    88621 gataacgccc cggcaattaa ggtctataaa aaatacggct ttgaaattga agggactggt
    88681 aagaagtacg cattgcgtaa tggtgaatat gtcgatgcat attatatggc gcgggtgaag
    88741 taagatagtg ccctttttct gagatggaaa aagggtgtca ttcaaaatcg acataccttc
    88801 ctttaaggta tttatttgcc caatacatat attgagattt aactcataat tacttcctaa
    88861 agtgtaatat tttatttttt aatatatacg cctacaattt cctggagtaa ataaataaca
    88921 attaacaagc ataatattgc cattgataaa atagcatgcc ataaaaggac ttttcaggga
    88981 tgagtaatat tgtttacctg acagtaacgg gagaacaaca aggaagcatc tccgcaggtt
    89041 gtgggacttc tgagtctaca ggtaatcgtt ggcagagcgg gcatgaggat gaaatattta
    89101 cattctcact cttaaataat attaataata cggggcttgg ttcacagttc catggtataa
    89161 cattttgtaa attaattgat aaaagcactc cattatttat taattccatt aacaataatg
    89221 aacaattatt tatgggattt gacttctatc gaataaatag atttggtaga tgggaaaagt
    89281 attattatat acaactaaga ggcgcttttt tatcggctat tcatcaccag atcattgaaa
    89341 accaactgga tacagaaaca ataactatta gttatgaatt tatcctctgt caacatctta
    89401 tcgcaaatac cgagttcagc tatttggcac tccctgaaaa ttataaccgt ttgtttttac
    89461 caaattcaaa aaaccaaaca aataatcgtt tcaaaacgtt aaacagcaaa gctattggca
    89521 ggctacttgc tgctggtggc gtatacaatg ggaacattga aggattcaga gatactgcgg
    89581 aaaaactggg tggagatgca ataaaaggct atgatcaaat actaaatgaa aaaacagcgg
    89641 gcatagcgat agcaacagca tctattcttt taacaaagcg ttctaatgtt ga
//
LOCUS       DAZFXT010000003        84433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.003, whole genome shotgun sequence.
ACCESSION   DAZFXT010000003 DAZFXT010000000
VERSION     DAZFXT010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.003"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..1749
                     /gene="yegH"
                     /locus_tag="ACHKW0_000190"
     CDS             166..1749
                     /gene="yegH"
                     /locus_tag="ACHKW0_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7473087.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2200..4053)
                     /gene="asmA"
                     /locus_tag="ACHKW0_000191"
     CDS             complement(2200..4053)
                     /gene="asmA"
                     /locus_tag="ACHKW0_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HFO7473088.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTYVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(4075..4656)
                     /gene="dcd"
                     /locus_tag="ACHKW0_000192"
     CDS             complement(4075..4656)
                     /gene="dcd"
                     /locus_tag="ACHKW0_000192"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HFO7473089.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4748..5389)
                     /gene="udk"
                     /locus_tag="ACHKW0_000193"
     CDS             complement(4748..5389)
                     /gene="udk"
                     /locus_tag="ACHKW0_000193"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HFO7473090.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5707..9024
                     /gene="dgcE"
                     /locus_tag="ACHKW0_000194"
     CDS             5707..9024
                     /gene="dgcE"
                     /locus_tag="ACHKW0_000194"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7473091.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(9063..9920)
                     /gene="alkA"
                     /locus_tag="ACHKW0_000195"
     CDS             complement(9063..9920)
                     /gene="alkA"
                     /locus_tag="ACHKW0_000195"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HFO7473092.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVAEDYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCVDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            10054..11406
                     /gene="yegD"
                     /locus_tag="ACHKW0_000196"
     CDS             10054..11406
                     /gene="yegD"
                     /locus_tag="ACHKW0_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7473093.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11418..13358)
                     /gene="yegI"
                     /locus_tag="ACHKW0_000197"
     CDS             complement(11418..13358)
                     /gene="yegI"
                     /locus_tag="ACHKW0_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HFO7473094.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPGDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILIEIALSALSLLLCGLQAEPRYIILVPVLSAIWIIGSLTSKAYKAEIQQRREAFNRA
                     KMDYEHLVSQIQQLGGLEGFIAKRAMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13355..14116)
                     /gene="pphC"
                     /locus_tag="ACHKW0_000198"
     CDS             complement(13355..14116)
                     /gene="pphC"
                     /locus_tag="ACHKW0_000198"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HFO7473095.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAYMSQKVQGGELGLNDILATDIVLTVRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(14113..14772)
                     /gene="yegL"
                     /locus_tag="ACHKW0_000199"
     CDS             complement(14113..14772)
                     /gene="yegL"
                     /locus_tag="ACHKW0_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7473096.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFRGEEDKRFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14993..15052)
                     /gene="ibsA"
                     /locus_tag="ACHKW0_000200"
     CDS             complement(14993..15052)
                     /gene="ibsA"
                     /locus_tag="ACHKW0_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HFO7473097.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15325..15381)
                     /locus_tag="ACHKW0_000201"
     CDS             complement(15325..15381)
                     /locus_tag="ACHKW0_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7473098.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15653..15709)
                     /locus_tag="ACHKW0_000202"
     CDS             complement(15653..15709)
                     /locus_tag="ACHKW0_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7473099.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15988..17235
                     /gene="mdtA"
                     /locus_tag="ACHKW0_000203"
     CDS             15988..17235
                     /gene="mdtA"
                     /locus_tag="ACHKW0_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HFO7473100.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLLFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLSSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17235..20357
                     /gene="mdtB"
                     /locus_tag="ACHKW0_000204"
     CDS             17235..20357
                     /gene="mdtB"
                     /locus_tag="ACHKW0_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HFO7473101.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALLIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPREAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SCFARHEEEA"
     gene            20358..23435
                     /gene="mdtC"
                     /locus_tag="ACHKW0_000205"
     CDS             20358..23435
                     /gene="mdtC"
                     /locus_tag="ACHKW0_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HFO7473102.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTADEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRSSLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRMRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23436..24851
                     /gene="mdtD"
                     /locus_tag="ACHKW0_000206"
     CDS             23436..24851
                     /gene="mdtD"
                     /locus_tag="ACHKW0_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7473103.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMALIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24848..26251
                     /gene="baeS"
                     /locus_tag="ACHKW0_000207"
     CDS             24848..26251
                     /gene="baeS"
                     /locus_tag="ACHKW0_000207"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HFO7473104.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26248..26970
                     /gene="baeR"
                     /locus_tag="ACHKW0_000208"
     CDS             26248..26970
                     /gene="baeR"
                     /locus_tag="ACHKW0_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HFO7473105.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            27161..27493
                     /gene="yegP"
                     /locus_tag="ACHKW0_000209"
     CDS             27161..27493
                     /gene="yegP"
                     /locus_tag="ACHKW0_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HFO7473106.1"
                     /translation="MAGWFELSESSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27702..27998
                     /locus_tag="ACHKW0_000210"
     CDS             27702..27998
                     /locus_tag="ACHKW0_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO7473107.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            28000..28296
                     /locus_tag="ACHKW0_000211"
     CDS             28000..28296
                     /locus_tag="ACHKW0_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7473108.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28399..29760
                     /gene="yegQ"
                     /locus_tag="ACHKW0_000212"
     CDS             28399..29760
                     /gene="yegQ"
                     /locus_tag="ACHKW0_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HFO7473109.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(30033..30251)
                     /gene="ogrK"
                     /locus_tag="ACHKW0_000213"
     CDS             complement(30033..30251)
                     /gene="ogrK"
                     /locus_tag="ACHKW0_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage transcriptional regulator OgrK"
                     /protein_id="HFO7473110.1"
                     /translation="MFHCPLCQHAAHARTSRYITDTTKERYHQCQNVNCSATFITYES
                     VQRYIVKPGEVHAVRPHPLPSGQQIMWM"
     gene            complement(30333..31496)
                     /locus_tag="ACHKW0_000214"
     CDS             complement(30333..31496)
                     /locus_tag="ACHKW0_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016157990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HFO7473111.1"
                     /translation="MLDALTFDAGSTLTPDYMLMLDSRDITGNISDRLMSMTLTDNRG
                     FEADQLDIELNDADGQVGLPVRGAVLTVYIGWKGFALVCKGKFTVDEVEHRGAPDVVT
                     IRARSADFRGTLNSRREGSWHDTTLGAIVEVIASRNKLEASVAPSLAGIKIPHIDQSQ
                     ESDAKFLTRLAERNGGEVSVKMGKLLFLKAGQGVTASGKKIPQITITRSDGDRHHFAI
                     ADRGAYTGVTAKWLHTKDPKPQKQKVKLKRKKKEKHLRALEHPKAKPVTQKKAPKVPE
                     AREGEYMAGEADNVFALTTVYATKAQAMRAAQAKWDKLQRGVAEFSISLATGRADIYT
                     ETPVKVSGFKRVIDEQDWTITKVTHFLNNSGFTTSLELEVMLSDVEYETEDDE"
     gene            complement(31496..31975)
                     /locus_tag="ACHKW0_000215"
     CDS             complement(31496..31975)
                     /locus_tag="ACHKW0_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7473112.1"
                     /translation="MMLALGMFVFMRQTLPHQTMQRESDYRWPSNSRIGKRDAYQFLG
                     VGEENMTLAGVLYPELTGGKLTMTTLRLMAEEGRAWPLLDGTGMIYGMYVISRVSETG
                     SIFFADGTPRKIDFTLSLTRVDESLAALYGDIGKQAESLIGKAGSMATKFTGMTGAE"
     gene            complement(31990..34437)
                     /locus_tag="ACHKW0_000216"
     CDS             complement(31990..34437)
                     /locus_tag="ACHKW0_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7473113.1"
                     /translation="MSNNVKLQVLLRAVDQASRPFKSIRTASKSLSGDIRETQKSLRE
                     LNGHASRIEGFRKTSAQLAVTGHALEKARQEAEALATQFKNTERPTRAQAKVLESAKR
                     AAEDLQAKYNRLTDSVKRQQRELAVVGINTRNLARDEQGLKNRISETTAQLNRQRDAL
                     ARVSAQQAKLNAVKQRYQAGKELAGNMASVGAAGVGIAAAGTMAGVKLLTPGYEFAQK
                     NSELQAVLGVAKDSAEMAALRKQARQLGDNTAASADDAAGAQIIIAKAGGDVDAIQAA
                     TPVTLNMALANRRTMEENAALLMGMKSAFQLSNDKVAHIGDVLSMTMNKTAADFDGMS
                     DALTYAAPVAKNAGVSIEETAAMVGALHDAKITGSMAGTGSRAVLSRLQAPTGKAWDA
                     LKELGVKTSDSKGNTRPVFTILKEMQASFEKNRLGTAQQAEYMKTIFGEEASSAAAVL
                     MTAASTGKLDKLTAAFKASDGKTAELVNIMQDNLGGDFKEFQSAYEAVGTDLFDQQEG
                     ALRKLTQTATKYVLKLDGWIQKNKSLASTIGLIVGGALALTGIIGAIGLVAWPVITGI
                     NAIIAAAGAMGAIFTTVGSAVMTAIGAISWPIVAVVAAIVAGALLIRKYWEPVSAFFG
                     GVVEGLKAAFAPVGELFTPLKPVFDWLGEKLQAAWQWFKNLIAPVKATQDTLNRCRDT
                     GVMFGQALADALMLPLNAFNKLRSGIDWVLEKLGVINKESDTLDQTAARTHAATYGTG
                     GYIPATSSYAGYQAYQPVTAPAGRSYVDQSKNEYHINLTGGTAPGTQLDRQLQDALEK
                     YERDKRARARASMMHDG"
     gene            complement(34430..34585)
                     /locus_tag="ACHKW0_000217"
     CDS             complement(34430..34585)
                     /locus_tag="ACHKW0_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO7473114.1"
                     /translation="MTFPKNLSVDDLMADVAVIFHWPPSELYPMSLTELITWREKALR
                     RSGNTNE"
     gene            complement(34582..34857)
                     /locus_tag="ACHKW0_000218"
     CDS             complement(34582..34857)
                     /locus_tag="ACHKW0_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001719230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO7473115.1"
                     /translation="MNKENVITLDNPVKRGEQVIEQVTLMKPSAGTLRGVSLAAVANS
                     EVDALIKVLPRMTAPMLTEQEVAALELPDLVALAGKVVGFLSPNSVQ"
     gene            complement(34914..35432)
                     /locus_tag="ACHKW0_000219"
     CDS             complement(34914..35432)
                     /locus_tag="ACHKW0_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO7473116.1"
                     /translation="MAMPRKLKLMNVFLNGYSYQGVAKSVTLPKLTRKLENYRGAGMN
                     GSAPVDLGLDDDALSMEWSLGGFPDSVIWELYAATGVDAVPIRFAGSYQRDDTGETVA
                     VEVVMRGRQKEIDTGEGKQGEDTESKISVVCTYFRLTMDGKELVEIDTINMIEKVNGV
                     DRLEQHRRNIGL"
     gene            complement(35445..36635)
                     /locus_tag="ACHKW0_000220"
     CDS             complement(35445..36635)
                     /locus_tag="ACHKW0_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HFO7473117.1"
                     /translation="MSDYHHGVQVLEINDGTRVISTVSTAIVGMVCTASDADAETFPL
                     NKPVLITNVQSAIAKAGKKGTLAASLQAIADQSKPVTVVVRVEDGTGDDEETKLAQTV
                     SNIIGTTDENGQYTGLKALLAAESVTGVKPRILGVPGLDTKEVAVALASVCQKLRAFG
                     YISAWGCKTISEVKAYRQNFSQRELMVIWPDFLAWDTVTSTTATAYATARALGLRARI
                     DQEQGWHKTLSNVGVNGVTGISASVFWDLQESGTDADLLNESGVTTLIRRDGFRFWGN
                     RTCSDDPLFLFENYTRTAQVLADTMAEAHMWAVDKPITATLIRDIVDGINAKFRELKT
                     NGYIVDATCWFSEESNDAETLKAGKLYIDYDYTPVPPLENLTLRQRITSRYLASLVTS
                     VNSN"
     gene            36962..37732
                     /locus_tag="ACHKW0_000221"
     CDS             36962..37732
                     /locus_tag="ACHKW0_000221"
                     /inference="COORDINATES: protein motif:HMM:NF039499.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5677 domain-containing protein"
                     /protein_id="HFO7473118.1"
                     /translation="MYKELIEQRLNEAKAARAALSKINGVIVEKTHNPDRGLYLVNYA
                     NLLMNRQIDIFDDALLLLENERYQSACIVSRGMIETHAFSRLLNKKVEKILTTQNGHA
                     SVDNAIEVILNFTNSSRFKKSEQEKIQKEVFDPNDYMFTEEAKYRFENLLAVSMHVMT
                     ALRELYKDEMKQTNQMESDFELTYDILSEYVHPSQTSIYHYYTPETHLVPTSVGNIHT
                     YVHAQLQCVRALLFILEAKSQHYWSTQLANEMTKRAKE"
     gene            complement(37923..38450)
                     /locus_tag="ACHKW0_000222"
     CDS             complement(37923..38450)
                     /locus_tag="ACHKW0_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001164091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7473119.1"
                     /translation="MQHLINITAGNPKTVEQYQLTKNFDVVWFFSEDGKNWYEEQKYF
                     ADDTIKIAYDKDNIIHYVEKDVTAIRPDGLSVVEVADITANRRADISGNWMFKDGKVI
                     KRIYTAEELQQQAEIRKARLLADAESVILPLERAVRLNMATDEERSRLESWERYSVLV
                     SRVDPANPEWPEMPQ"
     gene            complement(38712..38924)
                     /locus_tag="ACHKW0_000223"
                     /pseudo
     CDS             complement(38712..38924)
                     /locus_tag="ACHKW0_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(39735..40703)
                     /locus_tag="ACHKW0_000224"
                     /pseudo
     CDS             complement(39735..40703)
                     /locus_tag="ACHKW0_000224"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(40714..41244)
                     /locus_tag="ACHKW0_000225"
     CDS             complement(40714..41244)
                     /locus_tag="ACHKW0_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO7473120.1"
                     /translation="MSDSRLLPTGSSPLEVAAAKACAEIEKTPVSIRELWNPDTCPAN
                     LLPWLAWAFSVDRWDEKWPEATKRAVIRDAYFIHCHKGTIGAIRRVVEPLGYLINVTE
                     WWETSDPPGTFRLDIGVLESGITEAMYQEMERLIADAKPASRHLIGLNITRDIPGYLF
                     AGGVAYDGDVITVYPG"
     gene            complement(41237..42145)
                     /locus_tag="ACHKW0_000226"
     CDS             complement(41237..42145)
                     /locus_tag="ACHKW0_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HFO7473121.1"
                     /translation="MPIIDLNQLPAPDVVEELDFESILAERKATLISLYPEDQQEAVA
                     RTLTLESEPLVKLLEENAYRELIWRQRVNEAARAVMLACAAGNDLDVIGANYNTTRLT
                     ITPADDSTIPPTPAVMESDTDYRLRIQQAFEGLSVAGSVGAYQYHGRSADGRVADISV
                     TSPSPACVTISVLSRENNGVASEDLLAVVRNALNGEDVRPVADRVTVQSAAIVEYQIN
                     ATLYLYPGPESEPIRAAAVKKLEAYITAQHRLGRDIRLSAIYAALHVEGVQRVELAAP
                     LADIVLNSTQASFCTEYRVVTGGSDE"
     gene            complement(42150..42497)
                     /locus_tag="ACHKW0_000227"
     CDS             complement(42150..42497)
                     /locus_tag="ACHKW0_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO7473122.1"
                     /translation="MTARYLGMNRSDGLTVTDLEHISQSIGDILRTPVGSRVMRRDYG
                     SLLASMIDQPQTPALELQIKVACYMAVLKWEPRVTLSSVTTARSFDGRMTVTLTGQHN
                     DTGQPLSLTIPVS"
     gene            complement(42494..43129)
                     /locus_tag="ACHKW0_000228"
     CDS             complement(42494..43129)
                     /locus_tag="ACHKW0_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO7473123.1"
                     /translation="MNTLANIQELARALRNMIRTGLVVETDLNAGRCRVQTGGMCTDW
                     LQWLTHRAGRSRTWWAPSEGEQVLILSVGGELDTAFVLPGIYSGDNPAPSASADALHI
                     RFPDGAVIEYEPETSALTVSGIKTASVTASDSVTATVPVVTVKASTRVTLDTPEVVCT
                     NRLITGTLEVQKGGTMRGNIEHTGGELSSNGKVLHTHKHPGDSGGTTGGPL"
     gene            complement(43196..43648)
                     /locus_tag="ACHKW0_000229"
     CDS             complement(43196..43648)
                     /locus_tag="ACHKW0_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO7473124.1"
                     /translation="MNEFKRFEDRLTGLIESLSPSGRRRLSAELAKRLRQSQQRRVMA
                     QKAPDGTPYVPRQQQSARKKTGRVKRKMFAKLITSRFLHIRASPEQASMEFYGGKSPK
                     IASVHQFGLSEENRKDGKKIDYPARPLLGFTGEDVQMIEEIILAHLDR"
     gene            complement(43641..44108)
                     /locus_tag="ACHKW0_000230"
     CDS             complement(43641..44108)
                     /locus_tag="ACHKW0_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001406878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7473125.1"
                     /translation="MLKPDSLRRALTDAVTVLKTNPDMLRIFVDNGSIASTLATSLSF
                     EKRYTLNVIVTDFTGDFDLLIVPVLAWLRENQPDIMTTDEGQKKGFTFYADINNDSSF
                     DISISLMLTERTLVSEVDGALHVKNIPEPLPPEPVTRPMELYINGELVSKWDE"
     gene            complement(44071..44334)
                     /gene="lysC"
                     /locus_tag="ACHKW0_000231"
     CDS             complement(44071..44334)
                     /gene="lysC"
                     /locus_tag="ACHKW0_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796289.1"
                     /note="LysC is an Rz1-like component of a phage lytic
                     system, substantially overlapping although not fully
                     embedded in the gene for the Rz-like LysB component;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1-like lysis system protein LysC"
                     /protein_id="HFO7473126.1"
                     /translation="MALTCLMLCAGCTSAPPAPTPVIVHNACPKVSLCPMPGSDPQTN
                     GDLSADIRQLENALARCASQVKMIKHCQDENDAQTRQPAQGAD"
     gene            complement(44216..44497)
                     /gene="lysB"
                     /locus_tag="ACHKW0_000232"
     CDS             complement(44216..44497)
                     /gene="lysB"
                     /locus_tag="ACHKW0_000232"
                     /inference="COORDINATES: protein motif:HMM:TIGR03495.1"
                     /note="The gene for this Rz-like phage lysis system
                     protein may overlap extensively with the gene for the
                     other spanin subunit, the Rz1-like protein in the outer
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz-like lysis system protein LysB"
                     /protein_id="HFO7473127.1"
                     /translation="MLKNQLHVALTRADKNELAQVALRQELENAAKREAQREKAITRL
                     LNENEDFRRWYGADLPDAVRRLHQRPACTDASDCPQRLPESESLPDAGQ"
     gene            complement(44491..44634)
                     /locus_tag="ACHKW0_000233"
     CDS             complement(44491..44634)
                     /locus_tag="ACHKW0_000233"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473128.1"
                     /translation="MSRLMTVLVVLLSLAVAGLFLVKHKNDSLRASLDRANNVASRRP
                     SPC"
     gene            complement(44622..45047)
                     /locus_tag="ACHKW0_000234"
     CDS             complement(44622..45047)
                     /locus_tag="ACHKW0_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNZ54_00345 family protein"
                     /protein_id="HFO7473129.1"
                     /translation="MKKLSLSLMLNVSLALMLALSLIYPQSVAVSFVAAWAILATVIC
                     MVAGGVGVYATEYVLERYGRELPPESLAVKIVTSLFLQPVPWRRRAAALVVMVATFVS
                     LIAAGWIFTALIYLMTSLFFRLIRTACRQRIEGREPCQG"
     gene            complement(45062..45559)
                     /locus_tag="ACHKW0_000235"
     CDS             complement(45062..45559)
                     /locus_tag="ACHKW0_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 104 protein"
                     /protein_id="HFO7473130.1"
                     /translation="MPVINTHQNIAAFLDMLAVSEGTANHPLTKNRGYDVIVTGLDGK
                     PEIFTDYSDHPFAHGRPAKVFNRRGEKSTASGRYQQLYLFWPHYRKQLALPDFSPLSQ
                     DRLAIQLIRERGALDDIRAGRIERAISRCRNIWASLPGAGYGQREHSLEKLVTVWRTA
                     GGVPA"
     gene            complement(45559..45840)
                     /locus_tag="ACHKW0_000236"
     CDS             complement(45559..45840)
                     /locus_tag="ACHKW0_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7473131.1"
                     /translation="MTAEEKSVLSLFMIGVLIVVGKVLAGGEPITPRLFIGRMLLGGF
                     VSMVAGVVLVQFPDLSLPAVCGIGSMLGIAGYQVIEIAIQRRFKGRGKQ"
     gene            complement(45844..46047)
                     /locus_tag="ACHKW0_000237"
     CDS             complement(45844..46047)
                     /locus_tag="ACHKW0_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO7473132.1"
                     /translation="MKTFALQGDTLDAICVRYYGRTEGVVETVLATNPGLAELGAVLP
                     HGTAVELPDVQTAPVAETVNLWE"
     gene            complement(46047..46556)
                     /locus_tag="ACHKW0_000238"
     CDS             complement(46047..46556)
                     /locus_tag="ACHKW0_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO7473133.1"
                     /translation="MMTLIIPRKEAPVSGEGTVVIPQPAGDEPVIKNTFFFPDIDPKR
                     VRERMRLEQTVAPARLREAIKSGMAETNAELYEYREQKIAAGFTRLADVPADDIDGES
                     IKVFYYERAVCAMATASLYERYRGVDASAKGDKKADSIDSTIDELWRDMRWAVARIQD
                     KPRCIVSQI"
     gene            complement(46656..47399)
                     /locus_tag="ACHKW0_000239"
     CDS             complement(46656..47399)
                     /locus_tag="ACHKW0_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HFO7473134.1"
                     /translation="MTSPAQRHMMRVSAAMTAQREAAPLRHATVYEQMLVKLAADQRT
                     LKAIYSKELKAAKKRELLPFWLPWVNGVLEQGKGAQDDILMTVMLWRLDTGDIAGALE
                     IARYALKYGLTMPGKHRRTPPYMFTEEVALAAMRAHAAGESVDTRLLTDTLELTATAD
                     MPDEVRAKLHKITGLFLRDAGDAAGALAHLQRATQLDCQAGVKKEIERLERELKPKPE
                     PQPKAATRAPRKTRSVTPAKRGRPKKKAS"
     gene            complement(47403..48476)
                     /locus_tag="ACHKW0_000240"
     CDS             complement(47403..48476)
                     /locus_tag="ACHKW0_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001248534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO7473135.1"
                     /translation="MRQETRFKFNAYLSRVAELNGIDAGDVSKKFTVEPSVTQTLMNT
                     MQESSDFLTRINIVPVSEMKGEKIGIGVTGSIASTTDTAGGTERQPKDFSKLASNKYE
                     CDQINFDFYIRYKTLDLWARYQDFQLRIRNAIIKRQSLDFIMAGFNGVKRAETSDRNS
                     NPMLQDVAVGWLQKYRNEAPARVMSKVTDEEGHTTSEVIRVGKGGDYASLDALVMDAT
                     NNLIEPWYQEDPDLVVIVGRQLLADKYFPIVNKEQDNSEMLAADVIISQKRIGNLPAV
                     RVPYFPADAMLITKLENLSIYYMDDSHRRVIEENPKLDRVENYESMNIDYVVEDYAAG
                     CLVEKIKVGDFSTPAKATAEPGA"
     gene            complement(48535..49389)
                     /locus_tag="ACHKW0_000241"
     CDS             complement(48535..49389)
                     /locus_tag="ACHKW0_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001074418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO7473136.1"
                     /translation="MAKKVSKFFRIGVEGDTCDGRVISAQDIQEMAETFDPRVYGCRI
                     NLEHLRGILPDGIFKRYGDVAELKAEKIDDDSALKGKWALFAKITPTDDLIAMNKAAQ
                     KVYTSMEIQPNFANTGKCYLVGLAVTDDPASLGTEYLEFCRTAKHNPLNRFKLSPENL
                     ISVATPVELEFEDLPETVFTALTEKVKSIFGRKQASDDARLNDVHEAVTAVAEHVQEK
                     LSATEQRLAEMETAFSALKQDVTDRADETSQAFTRLKNSLDSTESLTQQRRSKATGGG
                     GDALLTNC"
     gene            49563..51335
                     /locus_tag="ACHKW0_000242"
     CDS             49563..51335
                     /locus_tag="ACHKW0_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HFO7473137.1"
                     /translation="MTITTDTTLLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSW
                     KQRDGWDSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQ
                     TGNEADLNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHR
                     IRDILKSRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVD
                     LTGDPIVLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSH
                     LRSTYFSTPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQ
                     IVTIEDALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTL
                     EEWEDYAPFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGM
                     DFATQAESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVL
                     KAKDVIRRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATM
                     HALLNEPLTAGISTPLTSTILEFY"
     gene            51335..52369
                     /locus_tag="ACHKW0_000243"
     CDS             51335..52369
                     /locus_tag="ACHKW0_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7473138.1"
                     /translation="MSKKKGKTPQPAAKKMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFTPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            52761..53744
                     /locus_tag="ACHKW0_000244"
     CDS             52761..53744
                     /locus_tag="ACHKW0_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990814 - DNA/DNA annealing activity
                     [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HFO7473139.1"
                     /translation="MTDIETARWNTESAALLALSEIHGVSYWTLYKVAQKGIRFRDIV
                     TSQTLANFEYLLGVKLHRQPYYLNESNWSVFRDRMISTAKILLTHYHNSGYKIIHHGS
                     PNYPDKLNDLSEPPFWLFAQGDVSLLDKKCVGVVGTRNPTALGIYLTQAVISQFIDSD
                     YSTVSGLAYGIDQSAHEASLLFKIPTIAVLGTGVNSNYPKNSGEMRGHIVNNGGLILT
                     EYLPNQKPSQENFVRRNRIQAALSDVLIPVEWGLKSGTSHTVRYAAQLKRAILCPLLR
                     GTTPQEEIKHALSEYSATIMNIPLSDFKDVQNLIKSASGVKTQQLSLLGDE"
     gene            53746..55020
                     /locus_tag="ACHKW0_000245"
     CDS             53746..55020
                     /locus_tag="ACHKW0_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473140.1"
                     /translation="MYLKAVIFSIADVVLPLTSNTQPQELKSRIDSELRKLFAFLSSK
                     GIKVIFLTNKNRNVETHDGVVTLDEYLKRKFPESIHFCHELDNNIPAKQTGKAIDFIM
                     AALELKRNEMIYVGRSREDLQAATNGNTLFINATWYEPVTEYGFQFSEPKEIARFIDV
                     FCLREQLWGWQGHFNEDVHYYALAPFSTYVTEFTMYSANARDLAKLSVGSPDFWIRYL
                     GASIYFSGLSEGASFITTYVGHNAENPYKLADIMEHDLKGLAVSFKGKYLKDLFLRHT
                     TAIKSQQARIAKQEVNITSQINTVHLNPAPIKNINTGKRYTNPPNLKGKKILVIDDFC
                     TEGNAHETARMYLKAAGAKVINISWLKTINRDVSICEPTRKIRPWDANTLDIDDINYV
                     GTIGYAENVTHGSAPQVLSEKIRQYDNWDWPQ"
     gene            complement(55195..57450)
                     /locus_tag="ACHKW0_000246"
     CDS             complement(55195..57450)
                     /locus_tag="ACHKW0_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7473141.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKTFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVGKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCHPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGRPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTNWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWSDPEVVRVLRGALKHDLRTPNRQ
                     QRNGSPLKPHEIAPSARLTRSERFQITRIRVDLAQNGIRPQRWELEALTRGATVNYDG
                     KKFKYPVIDEW"
     gene            complement(57440..57715)
                     /locus_tag="ACHKW0_000247"
     CDS             complement(57440..57715)
                     /locus_tag="ACHKW0_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO7473142.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSGLMHHDILTGSAKSFADLNAQVEQLSKRCSEAFGSYGR"
     gene            complement(57712..57936)
                     /locus_tag="ACHKW0_000248"
     CDS             complement(57712..57936)
                     /locus_tag="ACHKW0_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7473143.1"
                     /translation="MPDNVDFIQEQQAELLERQINAARVKHCGVSALVCEECDAPIPA
                     ARRAAYPSATRCVSCQSVFEAKNKHYRRTA"
     gene            complement(57936..58238)
                     /locus_tag="ACHKW0_000249"
     CDS             complement(57936..58238)
                     /locus_tag="ACHKW0_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HFO7473144.1"
                     /translation="MSCSRSVVLLNNALKIAVMKNGDLSLIQLGLDKEKREITESVIA
                     IYQNELNLLSDVVNLLVKRAVFHKQISSVDELTKLTTEIASYCADEFKKLNDKRSW"
     gene            complement(58238..58462)
                     /locus_tag="ACHKW0_000250"
     CDS             complement(58238..58462)
                     /locus_tag="ACHKW0_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HFO7473145.1"
                     /translation="MHTVSENQCGKYALLLQQARTEAQADAATRFSSHLDAMIRHITK
                     AELSRVEIVELLSQESEKFHNIGLSRGEVL"
     gene            complement(58526..59026)
                     /locus_tag="ACHKW0_000251"
     CDS             complement(58526..59026)
                     /locus_tag="ACHKW0_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein B"
                     /protein_id="HFO7473146.1"
                     /translation="MTVMTLNLVEKQPAAMRRIIGKHLAVPRWQDTCDYYNQMMERER
                     LTVCFHAQLKQRHATMCFEEMNDVERERLVCAIDELRGAFSKRRQVGASEYAYISFLT
                     VSQRRTLFMHAGLTEKEFNQPYWRINEESCYWRDALFRALRELFSLFEYAPTILTSVK
                     PEQYLH"
     gene            complement(59023..59193)
                     /locus_tag="ACHKW0_000252"
     CDS             complement(59023..59193)
                     /locus_tag="ACHKW0_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473147.1"
                     /translation="MNEPRCIAQLLRNESPRAIDFTITHGKGRKGIIIRTKKQSPLKK
                     ALTFLKSRRVWK"
     gene            complement(59204..59479)
                     /locus_tag="ACHKW0_000253"
     CDS             complement(59204..59479)
                     /locus_tag="ACHKW0_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory phage cox family protein"
                     /protein_id="HFO7473148.1"
                     /translation="MSKQVTLMTDAIPYQEFAKLIGKSTGAVRRMIDKGKLPVIDMTD
                     PQSASGRAGEYWVYLPAWNNGLKLAYESRPKEIRDGWLMWLGLGEPR"
     gene            59594..59893
                     /locus_tag="ACHKW0_000254"
     CDS             59594..59893
                     /locus_tag="ACHKW0_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7473149.1"
                     /translation="MSNTISEKIVLMRKSEYLSRQQLADLTGVPYGTLSYYESGRSTP
                     PTDVMMNILQTPQFTKYTLWFMTNQIAPESGQIAPALAHFGQNETTSPHSGQKTG"
     gene            60009..61022
                     /locus_tag="ACHKW0_000255"
     CDS             60009..61022
                     /locus_tag="ACHKW0_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001755162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HFO7473150.1"
                     /translation="MAIKKLDDGRYEVDIRPTGRNGKRIRRKFDKKSEAVAFEKYTLY
                     NHHNKEWLSKPTDKRRLSELTQIWWDLKGKHEEHGKSNLGKIEIFTKITNDPCAFQIT
                     KSLISQYCATRRSQGIKPSSINRDLTCISGMFTALIEAELFFGEHPIRGTKRLKEEKP
                     DTGYLTQEEIALLLAALDGDNKKIAILCLSTGARWGEAARLKAENIIHNRVTFVKTKT
                     NKPRTVPISEAVAKMIADNKRGFLFPDADYPRFRRTMKAIKPDLPMGQATHALRHSFA
                     THFMINGGSIITLQRILGHTRIEQTMVYAHFAPEYLQDAISLNPLRGGTEAESVHIVS
                     TVE"
     gene            complement(61287..61604)
                     /gene="yegR"
                     /locus_tag="ACHKW0_000256"
     CDS             complement(61287..61604)
                     /gene="yegR"
                     /locus_tag="ACHKW0_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YegR"
                     /protein_id="HFO7473151.1"
                     /translation="MKKIAAISLISIFIMSGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            62019..62918
                     /gene="yegS"
                     /locus_tag="ACHKW0_000257"
     CDS             62019..62918
                     /gene="yegS"
                     /locus_tag="ACHKW0_000257"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HFO7473152.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(63000..63773)
                     /locus_tag="ACHKW0_000258"
     CDS             complement(63000..63773)
                     /locus_tag="ACHKW0_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7473153.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(63879..64919)
                     /gene="gatD"
                     /locus_tag="ACHKW0_000259"
     CDS             complement(63879..64919)
                     /gene="gatD"
                     /locus_tag="ACHKW0_000259"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7473154.1"
                     /translation="MKSVVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFAQAVRDIA
                     RNAMPGKVLLIP"
     gene            complement(64967..66322)
                     /locus_tag="ACHKW0_000260"
     CDS             complement(64967..66322)
                     /locus_tag="ACHKW0_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015577 - galactitol transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015796 - galactitol transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO7473155.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKVVLA
                     E"
     gene            complement(66326..66610)
                     /gene="gatB"
                     /locus_tag="ACHKW0_000261"
     CDS             complement(66326..66610)
                     /gene="gatB"
                     /locus_tag="ACHKW0_000261"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HFO7473156.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARGDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(66641..67093)
                     /gene="gatA"
                     /locus_tag="ACHKW0_000262"
     CDS             complement(66641..67093)
                     /gene="gatA"
                     /locus_tag="ACHKW0_000262"
                     /EC_number="2.7.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HFO7473157.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(67103..68365)
                     /gene="gatZ"
                     /locus_tag="ACHKW0_000263"
     CDS             complement(67103..68365)
                     /gene="gatZ"
                     /locus_tag="ACHKW0_000263"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HFO7473158.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDTANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            complement(68406..69248)
                     /gene="gatY"
                     /locus_tag="ACHKW0_000264"
     CDS             complement(68406..69248)
                     /gene="gatY"
                     /locus_tag="ACHKW0_000264"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HFO7473159.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADAFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGC"
     gene            complement(69558..70610)
                     /gene="fbaB"
                     /locus_tag="ACHKW0_000265"
     CDS             complement(69558..70610)
                     /gene="fbaB"
                     /locus_tag="ACHKW0_000265"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HFO7473160.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            70867..72144
                     /gene="yegT"
                     /locus_tag="ACHKW0_000266"
     CDS             70867..72144
                     /gene="yegT"
                     /locus_tag="ACHKW0_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015858 - nucleoside transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease"
                     /protein_id="HFO7473161.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            72141..73145
                     /gene="yegU"
                     /locus_tag="ACHKW0_000267"
     CDS             72141..73145
                     /gene="yegU"
                     /locus_tag="ACHKW0_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HFO7473162.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            73142..74107
                     /gene="yegV"
                     /locus_tag="ACHKW0_000268"
     CDS             73142..74107
                     /gene="yegV"
                     /locus_tag="ACHKW0_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HFO7473163.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(74081..74827)
                     /gene="yegW"
                     /locus_tag="ACHKW0_000269"
     CDS             complement(74081..74827)
                     /gene="yegW"
                     /locus_tag="ACHKW0_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7473164.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYLQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(74879..75697)
                     /gene="yegX"
                     /locus_tag="ACHKW0_000270"
     CDS             complement(74879..75697)
                     /gene="yegX"
                     /locus_tag="ACHKW0_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HFO7473165.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGTVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQYSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            complement(75762..76562)
                     /gene="thiD"
                     /locus_tag="ACHKW0_000271"
     CDS             complement(75762..76562)
                     /gene="thiD"
                     /locus_tag="ACHKW0_000271"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HFO7473166.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            complement(76559..77347)
                     /gene="thiM"
                     /locus_tag="ACHKW0_000272"
     CDS             complement(76559..77347)
                     /gene="thiM"
                     /locus_tag="ACHKW0_000272"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HFO7473167.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     regulatory      complement(77382..77478)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(77570..77842)
                     /gene="rcnR"
                     /locus_tag="ACHKW0_000273"
     CDS             complement(77570..77842)
                     /gene="rcnR"
                     /locus_tag="ACHKW0_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HFO7473168.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     gene            77963..78787
                     /gene="rcnA"
                     /locus_tag="ACHKW0_000274"
     CDS             77963..78787
                     /gene="rcnA"
                     /locus_tag="ACHKW0_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HFO7473169.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLITVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            79006..79344
                     /gene="rcnB"
                     /locus_tag="ACHKW0_000275"
     CDS             79006..79344
                     /gene="rcnB"
                     /locus_tag="ACHKW0_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HFO7473170.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(79426..80460)
                     /gene="yehA"
                     /locus_tag="ACHKW0_000276"
     CDS             complement(79426..80460)
                     /gene="yehA"
                     /locus_tag="ACHKW0_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HFO7473171.1"
                     /translation="MNIRIMLFIVMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     MATYEHNFNDSSCVSTNTITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFIL
                     ESNGSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(80474..82954)
                     /gene="yehB"
                     /locus_tag="ACHKW0_000277"
     CDS             complement(80474..82954)
                     /gene="yehB"
                     /locus_tag="ACHKW0_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HFO7473172.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGRSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRFGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(82970..83644)
                     /gene="yehC"
                     /locus_tag="ACHKW0_000278"
     CDS             complement(82970..83644)
                     /gene="yehC"
                     /locus_tag="ACHKW0_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7473173.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(83725..84267)
                     /gene="yehD"
                     /locus_tag="ACHKW0_000279"
     CDS             complement(83725..84267)
                     /gene="yehD"
                     /locus_tag="ACHKW0_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7473174.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
BASE COUNT        20188 a        22363 c        20970 g        20912 t
ORIGIN      
        1 cttaaataat gttttattat taccactttt aattcaggga taatgcgatg ttataatcct
       61 caaataatgt gagataaata gtgccgcaac attgcatttt tgccctgatt tatccatgat
      121 cgaattgtga catttgtcat acaacgaata ggttttgtac ttactatgga atggattgcc
      181 gatccgtcta tctgggccgg gttaatcacg ctgattgtga tcgaactggt cctcggcatt
      241 gataacctgg tctttattgc catcctcgcc gaaaaactac cgccgaagca gcgcgaccgc
      301 gcacgggtta ccgggctgct gctggcgatg ttaatgcgcc tgttactgct ggcgtcaatc
      361 tcctggctgg tcaccctgac gcaaccgctg ttcagcttcc gctcgtttac ctttagcgcc
      421 cgcgacttaa tcatgctgtt tggtggtttc ttcctgttgt tcaaagccac gatggagctg
      481 aacgaacggc tggaaggaaa agacagcaat aaccccacac aacgcaaagg tgcgaagttc
      541 tggggggtgg tgacacaaat tgtggtactg gacgccatct tctcactcga ctcggtgatt
      601 accgccgtcg ggatggtcga ccatttactg gtcatgatgg ccgccgttgt tatcgctatc
      661 agcctgatgt tgatggccag caagccgtta acgcaatttg ttaacagtca cccgaccatc
      721 gttattctct gcttaagctt cctgttgatg attggcttta gcctggtggc agaaggtttc
      781 ggcttcgtca ttccgaaagg ctacctgtac gccgccattg gcttctcggt gatgatcgag
      841 gcgctcaatc agttggctat ctttaaccgg cgacgttttc tttccgctaa ccagacgcta
      901 cgccagcgga ctaccgaagc ggtaatgcgc ctgcttagcg ggcaaaaaga agatgcggaa
      961 ctggacgccg aaaccgcgtc catgctggtg gatcatggta accagcagat ctttaatccg
     1021 caggaacggc ggatgattga gcgggtactt aatcttaacc agcgtactgt cagcagcatt
     1081 atgacgtcgc gccacgatat tgagcatatc gatctcaacg cgccggaaga agaaatccgc
     1141 cagttgctgg agcgaaatca gcatacgcgg ctggtggtta ctgatggtga tgacgcagaa
     1201 gatttgctcg gtgttgttca cgttatcgac ctgctacaac agtctctgcg cggcgaaccg
     1261 ctcaacctgc gggtgttgat tcgccagcca ctggtgttcc cggaaacctt gccgttgtta
     1321 cctgccctcg agcagttccg taatgcccgc acacactttg cttttgtggt ggatgagttt
     1381 ggctcggtgg aagggattgt gacattaagt gacgtcactg aaaccattgc cggtaactta
     1441 ccgaacgaag tggaagagat cgacgcccgc catgatattc agaagaatgc ggacggttcc
     1501 tggacggcga atggtcatat gccgctggaa gatctggtgc aatatgtgcc gctgccgctg
     1561 gatgaaaaac gtgaatatca caccattgcc gggctgttga tggaatattt acagcgtatt
     1621 ccaaaacctg gcgaagaagt tcaggtgggg gattatttgc ttaaaacgtt gcaggtggaa
     1681 agccatcgcg tgcagaaggt gcaaattata ccgctacgta aggatggcga gatggagtac
     1741 gaggtgtgat ggtatcggcc attttaccca atgctgcgta acgccttata acttcagcaa
     1801 tgaagcgttg tctgtggtaa acggacatca cagcgggggt gtgggtccgg ctgtaaatcg
     1861 ccgaatcgtt tcctgtaggc cggggcagcc cacatggatg tgggctgagg gcgcgtctta
     1921 caggacgtta cctcgcgccc gtacccgata gccgaaagga ataagatgag ggaaccgcgc
     1981 cagcggcgat ttaccgccgg gagcccgggt tgccagggtg gtggcgattg agccaccctg
     2041 gcacgttcac cggcttgagc gttccatagt agcgaggaac atcaagtgaa cggaacagcc
     2101 ttgaatctta catattcaga atataatggt gccgccgcga acgcctgatg cgacgcttta
     2161 cgcgtcttat caggcctaca ggacaggtca cctggtaatc tacatcttct ccagcaactt
     2221 cttcacatct ttgccattgc gggaatcttt attccgctcg gcccagtcat tcaggcgacg
     2281 tttcgcttcg tcctgtagat gtttgcgcag cagctggtcc acttgcaggc tgtaattgag
     2341 ttgctgccag ttgccataaa cccgcagcgg tactggcgtt tctttgagga aatcaatcag
     2401 tttgctttcc ccgttccagc cacccacgac ccgaatatca aactgggtgt cgcaggtttg
     2461 atctgccaga ttcaacatac cttcccctgt cagcgccagc acaggcgatt gaccttgcat
     2521 gtcgtttaac gtcacgacgc catccttcaa tgtcaaatcg gtggtaaagc ggtcaagccg
     2581 cgtcacgtta tcgaagtttt cagcggcctt cacatcacca ccattacgtt ctaccgcttg
     2641 ctgaatcatc tgctggaagt tcatcccttc catgcgcgtg tcggtcattt cgacatgtgc
     2701 ctgtccttgc cagttatggc ggaatgcgtc ggcatctatg tcagcaccgg agaaatcacc
     2761 agccagtgac atctttccgg tcaacgaaat cggatagtta aacgccttca gaatggtacc
     2821 aatctcaacg ttttccagcc gtggctggaa gtttatccgc ggatttattg atgtcgcgtc
     2881 cagcgtgccc ggcagtgaaa cctttccacc gttaagcttg ccctgcagtt gagtaatttc
     2941 cagcaaaccc gacttgttgg tcatttgcgt ggcaacatat gtaaaattca ttccgcgcca
     3001 gcgcacgtta ctggcttgca acaaaatatc agccgtaaag ccttgcagtc cctgataggc
     3061 cggttcatca atacgcgaag aaataaccgg gcgcggcaac gttgattggc tctgcccctg
     3121 ctgcgcggca ccgttttcac cattcgcggt ttcattaagc gggatgaggt tgtcaagatt
     3181 cagttgcggg aattgcagtc tcagctgcca ttcaggtttc tctgtcagcg tgacctgtgc
     3241 ttgcccgctc agcgtgctgt cattggcagt caagctgatt tggttaaatg aaaggcgttt
     3301 atgtgactcc tgccactggg cctggaaact cccctgcccc tgaatacctt gttttggtaa
     3361 atctgcaccc tgcaactgcc agttaatttg ttcaatagcc gccgttaaat catgcggata
     3421 atcagaagca tctaccgtac cgttaaggga tatcgtgaga tcgcgctgat cgcgattaac
     3481 ccgcccggag aactcaaatg agccacgatg ttggggatct tgttccattt gcaggcggat
     3541 attgcggatt gtcacctgct cgtcatcttc atgctggaac accagcacgc tgtccgccac
     3601 cttaagactg gatatatcaa acgaccatcc gcgatcgtct gacagatccg gcaaggtatt
     3661 gtcgcgcggt gcaaccggag cgtcttcact gcgcaccgct tccgtctgcg gcgtcagttg
     3721 gatcactgcc ccttttagca tcacctgctt aacgctcagt tgatgactca gtagtggtaa
     3781 aagcgccacg tccagacgca tgttgtcggc gcgaaccagt ggctggcttg cgccctgggc
     3841 ggtgagagac attcgcccgg agaggatact aagctgcggc cagacgtgcc aacgcagtgg
     3901 cccgtcgagc tgcaattgat aaccgctacg tgcagcaact tgcttgacca tatagtcgcg
     3961 gaaatcattc ggattcacca gcaacactaa cgcagataac ccggccacca gcacgaccag
     4021 gagtatcatc agcgtcgtca gaaatcgtct catggtatcc tcaatgggcc tgaattagtc
     4081 tttatcgatt cggctggcta ccgcgccctg ctggttgcga tatttcgcat cttcacggcg
     4141 gttgtaaggt cgcgccgccg ggccggaaag cggctcaaag ctcagcgcac caattaacat
     4201 gcccggacgc agcgccagcg gcagcttacc ggagttgtag aactccagca caatgcaacc
     4261 agaccagccc ggatcgatac ggtgcgcggt gacgtgcacc atcagcccca gacgcgccaa
     4321 agaggaacgc ccgtccagcc agcccaccag atcggctggc agcgtcaccg actccagcgt
     4381 caccgccagc gccagctctc ctgggtgaag atagaacgcc tcgccctcgt cgagaacgat
     4441 ctcatcgctc atcacgcggt caagcgcggc gctcacttca tctttgggac cgctcagatc
     4501 gataaacgct gccgtgtgac cacggaaggt acgaaattta ttgcccaggc gtacatccac
     4561 cgtcgcgccg ttaatacgct ccactggcgg acgtgggttg atcgacaaac ggccttcatc
     4621 aagccaggct tcaatatctc ggtcacacag acgcatggca ttttctcctt tcgcgcatca
     4681 ctcccttaac gccaattacg tcaagggcat accaggttat cactgaacgg tacacaattt
     4741 atcaagctta ttcaaagaac tgacttattt tcgctttcaa tatatcgatc gcgatgcggt
     4801 ttttcccgcc gcgcggcacg ataatgtccg catattgttt agaaggctca atgaattgca
     4861 ggaacatcgg gcgcacggtt ttttgatatt gcgccatcac ggaatccatt gaacgcccac
     4921 gctcgttaac gtcacgcttg atgcggcgca tcaggcagat atccagcggg gtatcaacga
     4981 aaatggagaa gttaagttcg tcacgcaaac gcgcatccgt cagcaacaaa atgccttcga
     5041 gaatgatgac cttcttcggc tcaaccgtca ccgtttcttt catacgcgta tgttcaacat
     5101 agctgtaaac cggcaggtca attgccgagc cgcgtttcaa cgcttgtaaa tgctcaagca
     5161 acaggctgtg atccatcgcg ctgggatggt cgtagttggt cttaacgcgt tcttccatcg
     5221 acagatggct ttgatctttg taatagcagt cttcgggaat tacgccgatg tgttcatcac
     5281 cgacttgctc acgtaattca cgataaaggg tactggcaat aagactcttg ccggaagccg
     5341 atgcgccagc gataccgata atgacgcact gatgagactg atcagtcata tatttagcga
     5401 cctgattaac ctggatgtta ggaagggggc gacgaagcgc caaacgcggc aattataggg
     5461 atttcatccg cctgatacca gtcgaatagc gctgccgcgc gctcagagtt aattgttgac
     5521 gaagaattcc cggtggcaaa ttacgttgat cagttttata caaggtaaaa aatgttatac
     5581 gcagttgcgc aaattatccg cctttacgtc actttatgag caattcgcat ataaaatgta
     5641 aaacttttgt actagcataa acacagaaac gaatactggc gacctggtct tgcggataaa
     5701 gcggtaatga gcaaacaatc acagcatgta ttaattgccc tgccccaccc gctgcttcac
     5761 ctggtcagtt taggtttagt ctcgtttatc tttacccttt tctcgcttga gctttcgcag
     5821 tttggcaccc aactcgcccc actgtggttc ccgacgtcca tcatgatggt ggcgttttat
     5881 cgccatgccg ggcgcatgtg gccgggaatt gcgctgagct gctcgctggg aaatatcgcc
     5941 gcatccatcc tgcttttttc caccagctcg ctgaacatga cctggacgac catcaatatt
     6001 gttgaagccg tggtcggggc agtgctgcta cgtaaattgc tgccgtggta taaccccttg
     6061 caaaatctgg ctgactggct gcgtctggca ttcggcagcg ccattgttcc gcctctgcta
     6121 gggggtgttc tggttgtcct gctgacgccc ggagacgatc ctctcagggc atttttgata
     6181 tgggtactgt cagaatccat cggcgctctg gcactggtgc cgctgggatt gttatttaaa
     6241 ccacactatc tgctgcgcca tcgcaaccca cggttgcttt ttgagtcgct gctcacgtta
     6301 gccatcacac tgacgttaag ctggctttcg atgctgtatc tgccgtggcc ttttactttc
     6361 attattgtgc tgttgatgtg gagcgccgtg cgcctgccac gaatggaagc ctttttgatc
     6421 ttccttacca cggtgatgat ggtgtcgctg atgatggccg cggatccctc cctgcttgct
     6481 acgccgcgta catacctgat gagccatatg ccgtggctac cgtttttgct gatcctgctg
     6541 cccgccaaca tcatgacgat ggtgatgtat gcctttcgtg cggaacgcaa acacatttcc
     6601 gaaagcgaaa cccgttttcg gaacgccatg gaatattccg ctatcggtat ggcgttagtg
     6661 ggcaccgagg gacaatggct gcaaaccaac aaagcgctct gccagtttct cgggtacagt
     6721 caggaagagc tgcgcggact cacctttcag caactgacct ggccggagga tctcaataaa
     6781 gatctccaac aggttgaaaa gctgataagc ggtgaaataa acacctattc aatggaaaaa
     6841 cgctactaca accgcaatgg cgatgttgtc tgggcgttgc ttgccgtctc actggtgcgc
     6901 cacacggatg gcacgccgct ctattttatc gctcagattg aagacattaa cgagctaaaa
     6961 cgcaccgaac aggtgaatca gcaactgatg gagcgcatca cgctggctaa cgaagcgggc
     7021 gggattggca tctgggagtg ggagttgaag ccgaatattt ttagctggga taagcggatg
     7081 ttcgagctgt atgaaattcc tccacatatc aaaccgaact ggcaggtgtg gtacgagtgc
     7141 gtgctgccgg aagatcgcca gcatgccgaa aaagtgattc gtgattcgtt gcaatcacgc
     7201 tcgcccttta aactggaatt tcgcattacc gtaaaagacg gtattcgcca tatccgcgcc
     7261 ctcgccaacc gggtactgaa taaagaaggc gaagtcgaac gcctgctcgg cattaatatg
     7321 gatatgactg aggttaaaca gcttaacgag gcattgtttc aggaaaaaga gcgcctgcac
     7381 ataacgctgg actccatcgg cgaagccgtg gtctgtattg atatggcaat gaaaattacc
     7441 tttatgaatc cagtggcgga gaagatgagc ggctggacgc aggaagaagc attaggtgtt
     7501 ccgctcctga cggtgttgca tattaccttt ggcgacaatg gtcccctgat ggagaacatt
     7561 tacagtgccg acacctcacg ttccgcgatt gaacaagatg tggtgttgca ctgccggagc
     7621 ggcggcagct acgacgtgca ttacagtatt acgccgttaa gtactctgga cggcagcaat
     7681 attggttcgg ttctggtgat tcaggacgtt accgaatcac gcaaaatgct gcgccagctg
     7741 agctacagcg cctcccatga tgcactgacg catctcgcca atcgcgccag ttttgagaag
     7801 caactgcgta tgctgctgca aacggtaaac agtacacatc agcgacatgc cctggtgttt
     7861 atcgatcttg atcgctttaa agcggtgaat gacagcgccg ggcatgcggc gggcgacgct
     7921 ttgctgcgcg aactggcgtc gttgatgctg agtatgctgc gttccagcga cgtgctggcg
     7981 cgactcggtg gggatgaatt tggtctgctg ctaccagact gtaatgttga aagcgcgcgt
     8041 tttatcgcta cacgcattat cagtgccgtg aatgactatc actttatatg ggaaggccgt
     8101 gtgcatcggg taggtgccag tgccgggatt accttgattg atgacaacaa tcatcaggcg
     8161 gcagaagtga tgtcgcaggc tgatatcgcc tgttatgcct ccaaaaatgg tggacggggc
     8221 cgggtgacgg tttacgaacc acagcaagct gccgcacata gcgaacgggc ggcgatgtcg
     8281 cttgatgaac agtggcggat gattaaagag aatcagttga tgatgatggc ccacggtgtc
     8341 gcttcgccgc ggatcccgga agcgcgtaat ttgtggctga tttcgcttaa actctggagt
     8401 tgcgaaggcg agattattga tgaacaaaaa tttcgtcgta gcttcagcga tccggcactt
     8461 agccatgctc ttgaccgacg ggtattccac gaatttttcc agctggccgc aaaagcggtt
     8521 gccagtaaag gcataagcat cgccctcccc ctttccgttg ccggtttgag tagcgccacg
     8581 ctggtgaatg atctgcttga gcagctggaa aatagccctc taccaccacg gttattacat
     8641 ctgattattc cggcagacgc gattttagat cacgcagaaa gcgtgcaaaa actgcggctg
     8701 gcgggatgtc ggatagtgct cagccaggtg ggccgcgatc tgcaaatctt caactcgctg
     8761 aaagcgaata tggcagatta cctgctactt gatggtgagt tatgcgccaa cgtgcagggt
     8821 aatttgatgg atgagatgct gattacgatt attcaggggc acgctcagcg actcgggatg
     8881 aaaaccatcg ccgggccagt cgttttaccc ttagtgatgg atacgctttc tggcatcggc
     8941 gtcgatctga tttatggtga tgtgattgcc gatgcccaac cgctggattt gctggtgaat
     9001 agcagttatt tcgcgattaa ctgagggagc gtttgcccat tgcctgatgc gacgcatcag
     9061 gcctacaatt cgtccgttcc gtctggttgc cagccttccg tataccagat atgcaacagc
     9121 gcataagaac gccagggctt ccagcgctcg gcataacggc ggatttgtgc cggtgtcatc
     9181 cccggaaatc gctgtttaat cagataatca tccggcagaa aaacatcttt cgcctgccag
     9241 ccacgcaaag cgaaataatt cgccgtccag cgcccgatac ccggaaaagt ttgcagcgtt
     9301 ttcatcgcct gctccacatc gcccggaatt gtcattggta agctgccctc cagcgccgca
     9361 tttgccagat gaatcagtgc ctctgcccgt ttcaacggca tgcctaacgc ttttaatgcc
     9421 tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc agatatactc cggaaaatca
     9481 tccagccgtt cgccataaag ctgtacaact ttggcagtca attttgccgc catcgccacg
     9541 ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa aagcatcaac acagccgggt
     9601 aaacgcaatc cgggccgcgc cgcgcctaac ttgcccaacg caccgttaac aatctgcggg
     9661 ttacattgca gatcaaacag gcggctcatt ttcgccagac actcagcggc aacgggttct
     9721 aaacctgcac ttaaatttat gtgcagagta tggcgggcta tatccggaat agcagtcacc
     9781 acgccgcgat attcgcccac cgccagacta cgggcataat aatcctcagc gaccgtttcc
     9841 acaccactca ctgcacgggc ggcgagaaat cccaacatcc acgaccagtc atacggcggc
     9901 tgccagttca gggtatacat cgcatctcct tattcatccg ctttcagcat aaacgttatt
     9961 cagacgctac gctttgcttt catattccgg ttgtcgcgac agcaacattt cgctaaagtc
    10021 acgccccttc ttcaccggca tggggattaa ttcgtgttta ttggttttga ttacggtaca
    10081 gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc aattactgaa aatggaaaac
    10141 gacagcacgc tgctgccttc aatgctttgc gcgccaacgc gtgaatcggt aagcgaatgg
    10201 ctgtaccgcc atcatgatgt tccggcagac gacgatgaaa cgcaggcgct gttacgtcgg
    10261 gcgattcgtt ataaccgcga agaagatatc gatgttacgg cgaaaagcgt gcagttcggt
    10321 ctttcctcac tggcacagta cattgatgat ccagaagaag tgtggtttgt gaaatcacca
    10381 aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg tagcgctgtt tgaggatctg
    10441 gtctgcgcaa tgatgttgca cattcgccag caggcgcagg cgcagctgcc agaagcgatt
    10501 actcaggcgg tgattggtcg cccgatcaac ttccaggggc tgggcggcga tgaagcaaac
    10561 gcccaggcgc aagggattct ggaacgcgcg gcgaagcgtg ccggattcag ggacgtggta
    10621 ttccagtacg agccggtcgc ggctgggctg gattacgaag ccaccttgca ggaagaaaaa
    10681 cgggtgctgg tggtggatat cggcggtggt acgactgact gttcattgct gctgatgggg
    10741 ccgcagtggc gttcgcgtct cgatcgtgaa gccagcctgc tgggtcacag tggttgccgt
    10801 attggcggta acgatctgga catcgcactg gcgtttaaaa acctgatgcc gttactgggc
    10861 atgggtggcg aaaccgaaaa aggcatcgcc ctgccgattc tgccgtggtg gaatgcggtt
    10921 gccatcaacg acgtacctgc gcagagtgat ttctacagta gtgccaacgg tcgtctgctt
    10981 aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg ccctgctaca gaaagtctgg
    11041 cgtcagcgtt taagctatcg cctggtgcgt agcgcagaag agtgcaaaat tgctctttca
    11101 agcgtagcgg aaacccgcgc ctcactgccg tttatcagcg atgaactggc tacgctgatt
    11161 agccagcaag ggctggaaag cgccctcagc cagccactgg cgcggattct ggaacaggtg
    11221 caactggccc tggataacgc ccaggaaaaa ccggacgtta tctacctgac cggcggtagc
    11281 gcccgttcgc cgctgattaa aaaagcgctg gcagaacagt tgccgggaat tccgattgca
    11341 ggcggcgatg actttggctc cgtcaccgcc gggctggcac gctgggcgga agtggtgttt
    11401 cgttaaaaga aaatggctca gcagcggctt aaatctgcct gcgcctgagc cagtttttct
    11461 gccgcctgac gtaaaggttc catcaacggc atggtccgtg ctggagcatg aaggcggaat
    11521 cgctgcaact ctgtcgcgcc gacagtcaac gtcgattcca gccgatgtcg tttggcggtc
    11581 attttcgcca ttaccgcttg tctgtctgct ggcgttatcg cttcattcgg cctgaacaca
    11641 aaacggcgtt cacagctcgc tttccagtcg ataaccgcct gggtcagatg gtcgccaaac
    11701 cctttcactt gcttaacgcc acgacgggta acatctgcgg ctgtttcaat accaaaagac
    11761 cgtaacgccg ctttacgcgc agggccaacg ccgggaatag aggcaacatc aataaaaaat
    11821 ccctccagaa acttctgctt ctgccgttcc cttgcagtgt catgaagtgc tgccagagcg
    11881 cgtttctctt cttccggtaa cccgagaatt tcgtccttca ttttttcgag catcgcccgt
    11941 ttggcgataa aaccttccag cccgcccaac tgttggatct ggctgactaa atgctcatag
    12001 tccattttcg cgcgattaaa tgcctcacgg cgttgctgga tttctgcttt gtacgctttg
    12061 cttgtcagac tgccaataat ccagatagcc gacagcacag gaaccaaaat aatataacgc
    12121 ggttctgcct gaaggccgca aagcaacagc gataacgctg acagtgcgat ctcaatcaga
    12181 atgatgtatt cgcgccgtaa caggcctaaa ggaagcggcc tgccagtcgg ttggaaatga
    12241 tcgggtaatg gcaattgcaa tgctggcggt gctactgacg ccatcaccat cgcccagact
    12301 ttcgccagca caaaatcacc gccggtggta atgacctctt cgccgagatc aataaaatag
    12361 ataacgcctt gattatccag cgcacaccac gggcagtcgg tcaaatgagc gggataaaca
    12421 tgcattgccg aaacgggaca tttctttaac tgttggcgca gagaatcaag tgccgctacc
    12481 cacgctttag ccgtcggacg cccggttgcc acgccacttt ccgtgaatgc ctgctgaaac
    12541 atggcttcaa catcgcccgg taacatcgat agcggaatcg atcgtggcgg cggttttaaa
    12601 ccacgtcgct gattatctga cgcgtaggca taacggaaat gggtaatatc cgtctccagc
    12661 gcattacccg catcagagat aagcggcaca ccagaataag gatgccggcc accaaacaag
    12721 acgtgaaaaa tcagcaacgc aaggccaaaa ttatcgtgat tttctgtgcg ttcaaagccg
    12781 acaaatgacg acaaggtttg tagctctggc ggcgtaaaat gcgacacgcc gacttcgcat
    12841 aaatgcagtg tgccattggc gttaatctga aaggagtcgc tatcaatcag taccactttg
    12901 ctgtcgcgac ctaccataaa actgttctgg tttacgtcac ccacgacgtg cccgtgctcg
    12961 tgaaccgtag caaaagatga agcaatattg cgcgcaacat agagtagaaa atcccacgca
    13021 caatgagggt aacgttgacg acgatgtgcc gggctataga tcatatgaat cggttcttta
    13081 ccagaaactt ttggcatcat aaaaccgata acttttccgc ctcgtccacc gtgaagcgtt
    13141 gcctgcggcc aggcgacata attcaacaat tgcgcgtcag ctgtcgcagc cataaaggca
    13201 agtttgtcct gttttaaggc gggtggcggc gtgtgataaa tcttggcgac gctatcgaca
    13261 aactcattga tatcataaac cgcgccttca ccgcctttgc ccagttcacg gcctggcgta
    13321 acgcactcac cagtagcagt atataaagtg ggtttcatgg taaccacagc gccagtgcca
    13381 acgttttatc atcatccgta cgctcgttta ccgccgggct ggacaaaaac tgctttaaca
    13441 attcaggtaa taaatcgagt tgttcctgcg ttgctgacgc cagtccattg aaaaacgggg
    13501 taaagaaagg tacatgagga gaattatcca gcatatttaa cgccagtcgc tggataccat
    13561 ccgtaaatgc tgcaacttta tgcacacgct cagtgctggt gaaagtttcc agtctggaaa
    13621 cggcatcttc atcggtaatg aagtgcgtca tattggcata ttcacccacc atcggcgtaa
    13681 gcggcaattg cagaccgtga ccaaaatcca ccaccacgcc gccatcgcca atctgcataa
    13741 tcaaggtgcc attagccgat gaaatcaatc ccagaaacgt gcaggcaaaa tcgcgaactg
    13801 ctaattcttt ggcttccgcc tcggcaaaaa gtcgctgacg aacggtcagc acaatatccg
    13861 tggcgaggat atcattaagc cccaattccc caccctgcac tttttgcgac atataagcca
    13921 tcgcttcatt gacggcgagc gtcgcgcctt ctcctccctg cgaaacgcta cctgcgccat
    13981 cagcaaggaa catcaccagt aatggctgct ggtcatttag ccaggcaacc tgcatctggc
    14041 aggcatcctg gcaaggcaag tccgcgctga tgtgcgatgt tccaacagcg gatgcataaa
    14101 ccagacgcca gctcacactg acgtccagcc ttttggcgct tccagcacaa cttccgttcc
    14161 cggcgtggag cgggaaaccg aacgcagcga actggataac cagctaaaca gttcacggaa
    14221 ttgtaatcct tgcagaggca aaggctgacg aacgctgatt tgtgccagag tcttcatatc
    14281 cgccccctga acaccgatgg aaaagaaagc aaacctttta tcttcttccc cccgaaacac
    14341 tttgttggct gcagcctgcc actcatcggt tggtgcacca tcggtaatca ggaaaatcca
    14401 cgggcgataa tacgaaatac catttgcacg atactcacgt ttccgctcct ctaccatatc
    14461 cagggctttg gtaatggccg cacccattgg tgtatcgccc tgggcaaaca ggatgggcgg
    14521 aaaaaaatta gcggcgctgg taaagggttg ttccacatgc accgggccga acgtcacaat
    14581 gccgagttcc actcttttta aagccagcgg atcggcaagc agttcatcac gaaaggtcac
    14641 caatccggcg ttaagttcat taataggtct gccgctcatc gagccagaaa catccagcag
    14701 caaaatgcag gggcaacgtg gttccgggtt actggcaaaa tcgctggtgg caaacgtgat
    14761 ttgttcgctc ataagaaatc ctttcttaga gaattatcga ggtccggtat cataccgact
    14821 gcaataatga aataagagca aacgaagaaa ctttcagaat taggcccggc ggcgcagaaa
    14881 attttgctat tcgcctcgca agaacggaga gccgtcgatt gacactaagg gcggagtgac
    14941 ataatttcag gagtgagggt tagggagagg tttccccctc cccctggtgt tcttagtaag
    15001 cctggaagct aatcactaag agtatcacca gtatgatgac gtgcttcatc ataacccttt
    15061 ccttattaaa agccctcttc tccgggagag gctttcccgt ttcagcgtcc cgctgaaatc
    15121 atcggcttac ctcctttcgc cctgaatgca gtctatcgct aacgcgtgaa taaaatagtt
    15181 tcctctgtga ttattgcgta acgactcgca aagaatattt gctggcagga tcgcagacta
    15241 caaagcctgc ggattgacaa tcttatcgtg aaggcatact ttcaggagtg agggtagagc
    15301 ggggtttccc ccgccctggt agtcttagta agcggggaag cttatgacta agagcaccac
    15361 gatgatgagt agcttcatca tgaccctttc cttatttaag gccccttcct cgggaggggc
    15421 tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct cctttcgcca tgcacttagt
    15481 ctatcgctga cttgcagata aaatagtttc cagtgtgatt attgcgtaac gtctcgcaaa
    15541 gaatttttgc tggcaggatc gcagacggca aagcctgcgg attgacaatc ttatcgtgaa
    15601 ggcatacttt caggagtgag ggtagagcgg ggtttccccc gccctggtag tcttagtatg
    15661 ctgggtagct aatcactaag agcaccacga tgatgagtag cttcatcatg accctttcct
    15721 tatttaaggc cccttcctcg ggaggggctt tcccgtttca gcatccggct gaaatctgcg
    15781 gcttacctcc tttcgccctg tatgcagtct atcgctaacg cgtagataaa atagtttcct
    15841 gtgttattac tggatgcgtg ctcgcaaatg tgcccgccat tcagacgatt cccgatagtg
    15901 tttcataatt cctccatttt tctcccttat tggctggcta cactagtatc attccgcgaa
    15961 acgtttcagg aagagaaact cttaacgatg aaaggcagtt ataaatcccg ttgggtaatc
    16021 gtaatcgtgg tggttatcgc cgccatcgcc gcattctggt tctggcaagg ccgcaatgac
    16081 tcccagagtg cagccccagg ggcgacgaaa caagcgcagc aatcgccagc gggtggtcgc
    16141 cgtggtatgc gttccggccc attagccccg gttcaggcgg cgaccgccgt agaacaggca
    16201 gttccgcgtt acctcaccgg gcttggcacc attaccgccg ctaatactgt tacggtgcgc
    16261 agccgcgtgg acggtcaact gatggcgtta catttccagg aaggccagca ggtcaaagca
    16321 ggcgatttac tggcagaaat tgaccccagc cagttcaaag ttgcattagc acaagcccag
    16381 ggccaactgg caaaagataa agccacgctt gctaacgccc gccgtgatct ggcgcgttat
    16441 cagcaactgg caaaaaccaa tctcgtctcc cgtcaggagc tggatgccca acaggcgctg
    16501 gtcagtgaaa ccgaaggcac cattaaggct gatgaagcaa gcgtcgccag cgcgcagctg
    16561 caactcgact ggagccgcat caccgcacca gtcgatggtc gcgttggtct caagcaggtt
    16621 gatgttggta accaaatctc cagtggtgat accaccggga tcgtggtgat cacccagacg
    16681 catcctatcg atttgctctt taccctgccg gaaagcgata tcgctaccgt cgtgcaggcg
    16741 cagaaagccg gaaaaccgct ggtggtagaa gcctgggatc gcaccaactc gaagaaatta
    16801 agtgaaggca cgctgttaag cctcgataac caaatcgatg ccacaaccgg tacgattaaa
    16861 gtgaaagcac gctttaataa tcaggatgat gcgctgtttc ccaatcagtt tgttaacgcg
    16921 cgcatgttag tcgacaccga acaaaacgcc gtagtgatcc caacggccgc cctgcaaatg
    16981 ggcaatgaag gccattttgt ctgggtgctg agtagcgaaa acaaggtcag caaacatcta
    17041 gtgacgccgg gcattcagga cagtcagaaa gtggtgatcc gtgcaggtat ttctgcgggc
    17101 gatcgcgtgg tgacagacgg cattgatcgc ctgaccgaag gggcgaaagt ggaagtggtg
    17161 gaagcccaga gcgccactac tccggaagag aaagccacca gccgcgaata cgcgaaaaaa
    17221 ggagcacgct cctgatgcag gtgttacccc cgagcagcac aggcggcccg tcgcgcctgt
    17281 ttattatgcg tcctgtggcc accacgctgc tgatggtggc gatcttactc gccgggatta
    17341 tcggttatcg cgccctgccc gtttcggcgc tgccggaagt ggactatccg accattcagg
    17401 tggtcacgct ctacccaggt gccagcccgg atgtcatgac ctctgccgtt accgcgccgc
    17461 tcgaacgcca gttcgggcag atgtctggcc tgaaacagat gtcgtcgcaa agttccggcg
    17521 gtgcgtcagt tatcactttg cagttccagc taacattacc gctcgatgtc gccgagcagg
    17581 aagtgcaggc cgcgattaac gctgcgacca acttgttgcc gagcgatctg cctaacccgc
    17641 cggtttacag caaagtgaac ccggcagatc cgccgatcat gacgctcgcc gtcacctcaa
    17701 ccgccatgcc gatgacccag gtagaagata tggtggaaac ccgcgtcgcg cagaaaatct
    17761 cgcagatttc cggtgtcgga ctggtgacgc tttccggcgg tcagcgtccg gctgttcgcg
    17821 tcaaacttaa cgcccaggcg attgccgccc tcggcctgac cagcgaaacc gtgcgcaccg
    17881 ccattaccgg agctaacgtc aactcggcaa aaggtagcct cgacggccct tcccgtgcgg
    17941 tcacgctttc cgctaacgac cagatgcaat ccgccgaaga gtatcgccag ctaatcatcg
    18001 cctaccagaa cggcgcaccg attcgtctgg gcgatgtcgc aaccgtagag caaggagcag
    18061 aaaacagctg gctcggcgcg tgggcgaaca aagaacaggc cattgtgatg aatgttcagc
    18121 gccagcccgg tgctaacatt atctccaccg ccgacagcat ccggcagatg ctgccacagc
    18181 tcaccgagag tctgccgaaa tcggtgaagg tgacagtgct ttccgatcgc accaccaata
    18241 ttcgcgcatc cgtcgatgat actcagtttg aattgatgat ggctatcgcg ctggtagtca
    18301 tgatcatcta cctgtttttg cgcaatattc cggcgaccat cattcccggt gttgctgtac
    18361 cgctgtcgtt aatcggcact tttgcggtta tggtgtttct cgatttttca atcaataacc
    18421 tgacactgat ggcgttaact atcgccaccg gattcgtggt cgatgacgcc atcgtagtga
    18481 tcgaaaacat ttcccgctat atcgaaaaag gcgaaaaacc gttggcggcg gcgctcaaag
    18541 gcgcgggtga aatcggcttt accattatct cgctgacctt ctcgctgatt gcggtgttga
    18601 ttccactgct gtttatgggc gatatcgtcg ggcgactgtt ccgcgaattt gctattaccc
    18661 tggcggtagc gattttgatc tcagcggtgg tgtcgctgac cctgacaccg atgatgtgcg
    18721 cgcggatgct cagccaggag tcgctgcgta aacagaaccg attctcccgt gcctcggaaa
    18781 aaatgttcga caggataatc gccgcctatg gtcgtggact ggcgaaggtg ctgaatcatc
    18841 cgtggctgac cttaagcgtg gcgctcagca cgctgctgct tagcgttctc ttatgggtgt
    18901 tcattccgaa aggtttcttc ccggtacagg acaacggcat tattcagggc actttgcagg
    18961 caccgcaatc cagctccttt gccaatatgg cccagcgaca acgccaggtc gcggacgtga
    19021 ttttgcagga tccggcagtg caaagcctga cctcatttgt tggcgttgat ggcactaacc
    19081 cgtcgctgaa tagtgcacgt ttacaaatca acctcaaacc gctggatgaa cgtgatgacc
    19141 gagtgcaaaa agtcattgcc cgtctgcaaa cggcggtgga taaagtgccg ggcgtcgatc
    19201 tcttcctgca accaacgcag gatctgacta ttgatactca ggtcagccgc acccagtacc
    19261 agtttacctt gcaggccacc tcactggatg cgctcagtac ctgggtgcca cagttgatgg
    19321 aaaaactcca gcaactgcca cagctttctg atgtctccag cgactggcag gacaaagggc
    19381 tggtggcgta tgtcaatgtt gatcgcgaca gcgccagccg tctggggatc agcatggcgg
    19441 atgtcgataa cgccctgtac aacgcgtttg gtcagcgact gatttccact atttatactc
    19501 aggctaacca gtatcgcgta gtgctggagc acaacaccga aattaccccg ggcctcgcgg
    19561 cgctggatac cattcgcctg accagtagcg acggcggcgt ggtgccgtta agctcaattg
    19621 ccaaagttga acagcgtttt gcgccgcttt ccatcaacca tctggatcag ttcccggtaa
    19681 cgaccatctc ctttaacgtg ccggataact attcgctggg cgatgcggtg caggcgatta
    19741 tggacaccga aaagacgctg aatctgccgg tggatatcac cacgcagttc cagggcagca
    19801 ccctcgcctt ccagtcggcg ctgggcagca ctgtctggct gattgtcgcg gcggtggtgg
    19861 cgatgtatat cgtgctcggc attctgtacg agagttttat tcacccgatc accattctct
    19921 cgacgctacc caccgcaggg gttggcgcac tgctggcgtt gctgattgct ggtagcgaac
    19981 tggatgtgat tgcgattatc ggcattattt tgctgatcgg tatcgtgaag aagaacgcca
    20041 tcatgatgat cgacttcgcg ctggctgccg agcgcgagca aggcatgtcg ccgcgcgagg
    20101 caatctacca ggcttgtctg ttgcgttttc gtccgatcct gatgaccact ctggcggctc
    20161 tgcttggcgc gctgccgctg atgttgagta ccggggtcgg cgcggaactg cgtcgtccgt
    20221 taggtatcgg catggtcggc ggtctgattg tcagccaggt gctgacgctg tttaccacgc
    20281 cggtgattta tttgctgttc gaccgcctgg cattgtggac caaaagctgc tttgcccgtc
    20341 atgaagagga ggcgtaagtg aagttttttg ccctcttcat ttaccgcccg gtggcgacga
    20401 ttttactgtc ggttgccatt accctgtgcg gcatactggg cttccgtatg ctgccggtcg
    20461 ccccgctgcc gcaggtcgat tttccggtga ttatggtcag cgcctcgctg cccggtgcgt
    20521 caccagaaac aatggcgtct tccgttgcca cgccgctgga gcgctcactt gggcgcattg
    20581 ccggagtcag tgaaatgacc tccagcagtt cgctcggcag cacgcgtatt attttgcagt
    20641 ttgattttga ccgggatatc aacggcgcgg cgcgcgatgt gcaggcggcg atcaacgctg
    20701 cacaaagttt gctgcccagc gggatgccca gccgtccgac ctatcgcaaa gctaacccgt
    20761 cggatgcgcc aattatgatc ctcacgctga catccgatac ttattcgcag ggtgaactgt
    20821 acgattttgc ctcgacgcag ctggctccga cgatttcgca aatcgacggt gttggtgatg
    20881 tcgatgtcgg cggcagctca ctgcccgccg tacgcgtcgg gctgaatccg caggcgctgt
    20941 ttaatcaggg cgtctcgctg gacgacgtac gcactgccat cagcaatgcc aacgtgcgta
    21001 aaccgcaggg ggcgctggaa gatggcactc accgctggca gatccagacc aatgatgagc
    21061 taaaaaccgc cgacgaatat cagccgctga ttattcatta caacaacggc ggcgcggttc
    21121 gtctgggcga tgtggcgaca gtgaccgact ctgtacagga tgtacgtaac gccgggatga
    21181 ccaatgccaa accggctatt ttgctgatga tccgcaagct gccggaagcc aatattatcc
    21241 agacggttga cagcatccgg gcaaaattac cggagttgca ggaaaccatt ccggcggcga
    21301 ttgatctgca aattgcccag gatcgctccc ccaccattcg ctcctcgctg gaagaagtcg
    21361 agcaaacgct gattatctcg gtggcgctgg tgattctggt ggtattttta ttcctgcgct
    21421 cgggtcgcgc cactattatt cccgccgttt cggtgccggt ttcgctgatt ggtacgtttg
    21481 cggcgatgta cctgtgcgga ttcagtctca ataacctttc gttaatggcg ctcaccatcg
    21541 ctactggttt cgtggtggat gacgccatcg tggtgctgga aaacattgcc cgtcatctgg
    21601 aagcgggaat gaaaccgttg caagccgcac tgcaaggtac tcgcgaagtc ggttttacgg
    21661 tgctgtcgat gagtctgtca ctggtggcgg tgttcctgcc gctgctgttg atgggcggat
    21721 tgccgggccg actgttacgc gaatttgccg tgacgctttc tgtcgccatt ggtatatcgt
    21781 tgctggtttc tctgacatta acgccaatga tgtgtggctg gatgctgaaa gccagcaagc
    21841 cgcgcgagca aaagcgaatg cgtggttttg gtcgcatgtt ggtagccctg caacaaggct
    21901 acggcaagtc actgaaatgg gtgctcaatc atacccgtct ggtgggcgtg gtgctgcttg
    21961 gcaccattgc gctgaatatc tggctgtata tctcgatccc gaaaaccttc ttcccggagc
    22021 aggacacagg cgtgttgatg ggcggtattc aggcggatca gagtatttcg tttcaggcga
    22081 tgcgcggtaa gttgcaggat ttcatgaaaa ttatccgtga cgatccggca gtggataatg
    22141 tcaccggctt tacaggcggt tcgcgagtga acagcgggat gatgtttatc accctcaagc
    22201 cacgcgacga acgcagcgaa acggcgcagc aaattatcga ccgtctgcgc gtaaaactgg
    22261 cgaaagaacc gggggcgaat ctgttcctga tggcggtaca ggatattcgc gttggtgggc
    22321 gtcagtcgaa cgccagctac cagtacacgt tgttatccga cgacctggcg gcactgcgcg
    22381 aatgggagcc gaaaatccgc aaaaaactgg cgacgttgcc ggaactggcg gacgtgaact
    22441 ccgatcagca ggataacggc gcggagatga atctggttta cgaccgtgac accatggcac
    22501 ggctgggaat cgacgtacaa gccgccaaca gtctgttaaa taacgccttc ggtcagcggc
    22561 aaatctcgac catttaccag ccgatgaacc agtataaagt ggtgatggaa gtggatccgc
    22621 gctataccca ggacatcagt gcgctggaaa aaatgttcgt tatcaataac gaaggcaaag
    22681 cgatcccgct gtcgtatttc gctaaatggc aaccggcgaa tgccccacta tcggtgaatc
    22741 atcagggatt atcggcggcc tcgaccattt cgtttaacct gccgaccgga aaatcgctct
    22801 cggacgccag tgcggcgatc gatcgcgcaa tgacccagct tggtgtgcct tcgacggtgc
    22861 gcggcagttt tgccggaaca gcgcaggtgt tccaggagac gatgaactca caggtgatcc
    22921 tgattatcgc cgccatcgcc acggtgtata tcgtgctggg tatcctttac gagagttatg
    22981 tgcacccgct gacgattctc tccaccctgc cctcggcggg tgttggagcg ctgctggcgc
    23041 tggagctgtt caatgccccg ttcagcctaa tcgccctgat agggatcatg ctattaatcg
    23101 gcatcgtgaa gaaaaacgcc attatgatgg tcgattttgc gcttgaagcc caacggcacg
    23161 gtaacctgac gccgcaggaa gctattttcc aggcctgtct gctgcgtttt cgcccgatta
    23221 tgatgactac cctggcggcg ctgtttggcg cgctgccgct ggtgttgtcg ggcggtgacg
    23281 gctcggagct gcggcaaccc ctggggatca ccattgtcgg cggtctggta atgagccagc
    23341 ttctgacgct gtataccacg ccggtggtgt atctcttttt cgaccgtctg cggctgcgtt
    23401 tttcgcgtaa acccaaacaa acggtaaccg agtaaatgac agatcttccc gacagcaccc
    23461 gctggcaatt gtggattgtg gctttcggct tctttatgca gtcgctggac accaccatcg
    23521 taaacaccgc ccttccctca atggcgcaaa gcctcgggga aagtccgttg catatgcaca
    23581 tggtcattgt ctcttatgtg ctgaccgtgg cggtgatgct gcccgccagc ggctggctgg
    23641 cggacaaagt cggcgtgcgc aatattttct ttaccgccat cgtgctgttt actctcggtt
    23701 cactgttttg cgcgctttcc ggcacgctga acgaactgtt gctggcacgc gcgttacagg
    23761 gcgttggcgg cgcgatgatg gtgccggtcg gcagattgac ggtgatgaaa atcgtaccgc
    23821 gcgagcaata tatggcggcg atgacctttg tcacgttacc cggtcaggtc ggtccgctgc
    23881 tcggtccggc gctcggcggt ctgctggtgg agtacgcgtc gtggcactgg atctttttga
    23941 tcaacattcc ggtggggatt atcggtgcga tcgccacatt gatgttaatg ccgaactata
    24001 ccatgcagac gcggcgcttc gatctctccg gctttttatt gctggcggtt ggcatggcgg
    24061 tattaaccct ggcgctggac ggcagtaaag gtacaggttt atcgccgctg acgattgctg
    24121 gcctggtcgc agttggcgtg gtagcactgg tgctttattt gctgcacgcc agaaataaca
    24181 accgcgctct tttcagtctg aaactgttcc gcactcgtac cttttcgctg ggcctggcgg
    24241 ggagctttgc cggacgtatt ggcagtggca tgttgccctt tatgacaccg gttttcctgc
    24301 aaattggcct cggtttctca ccgtttcatg ccggactgat gatgatcccg atggtgcttg
    24361 gcagcatggg aatgaagcga attgtggtac aggtggtgaa tcgctttggt tatcgtcggg
    24421 tactggtagc gaccacgctg ggtctgtcgc tggtcaccct gttgtttatg actaccgccc
    24481 tgctgggctg gtactacgtt ttgccgttcg tcctgttttt acaagggatg gtcaactcga
    24541 cgcgtttctc ctccatgaac accctgacgc tgaaagatct cccggacaat ctggcgagca
    24601 gcggcaacag cctgctgtcg atgattatgc aattgtcgat gagtatcggc gtcactatcg
    24661 ccgggctgtt gctgggactt tttggttcac agcatgtcag cgtcgacagc ggcaccacac
    24721 aaaccgtctt tatgtacacc tggcttagca tggcgttgat catcgccctt ccggcgttca
    24781 tctttgccag agtgccgaac gatacgcatc aaaatgtagc tatttcgcgg cgaaaaagga
    24841 gcgcgcaatg aagttctggc gacccggtat taccggcaaa ctgtttctgg cgattttcgc
    24901 cacctgcatt gtcttgctga tcagtatgca ctgggcggtg cgtatcagtt ttgagcgtgg
    24961 ctttattgat tacatcaagc atggtaatga acagcgatta caactgttaa gtgatgcgct
    25021 tggcgagcag tatgcgcagc atggcaactg gcgcttcctg cgcaacaatg atcgctttgt
    25081 ctttcagatc ctgcgttcat ttgaacacga taattcggaa gataaacccg gcccgggtat
    25141 gccaccgcac ggctggcgta cccagttctg ggtggttgat caaaacaaca aagtgctggt
    25201 tggtccgcga gcgccgattc cacctgacgg tacacggcga cccattctgg tcaacggtgc
    25261 ggaagttggc gcggtgatcg cctcccccgt tgagcggtta acgcgcaata ctgatatcaa
    25321 tttcgataaa caacagcggc aaaccagctg gttgattgtc gctctggcaa cgttactcgc
    25381 ggcgctcgcc acctttctgc tggcgcgcgg tttactggca ccggtaaaac gacttgtcga
    25441 tggcacgcac aaactggcgg cgggcgattt cactacccgc gtaacgccca ccagtgaaga
    25501 tgaactgggc aaactggcgc aagacttcaa ccagcttgcc agcacactgg agaaaaacca
    25561 gcaaatgcgg cgcgatttta tggccgatat ttctcacgaa ctgcgtacgc cattagcggt
    25621 gctgcgcggt gaactggaag ccattcagga tggcgtgcgt aaattcacgc cggagacggt
    25681 ggcgtcttta caggcggagg tcggtacact gaccaaactg gttgacgatc tccatcagtt
    25741 gtcgatgtct gatgaaggcg ctctcgccta tcaaaaagca ccggtagatt tgatcccact
    25801 gctggaagtg gcgggcggcg catttcgcga acgattcgcc agtcgtggcc tgaaactgca
    25861 attttccctg ccagacagta ttaccgtatt tggcgatcgc gaccgtttaa tgcagttatt
    25921 caataactta ctggaaaaca gcctgcgcta cactgacagc ggcggcagcc tgcaaatctc
    25981 tgccgggcag cgcgacaaaa cggtgcgcct gacctttgcc gacagtgcgc caggtgtcag
    26041 tgacgatcag ctacaaaaat tgtttgaacg tttttatcgc accgaaggtt cccgcaaccg
    26101 tgccagcggc ggttccgggc tggggctggc gatttgcctg aacattgttg aagcacataa
    26161 tggtcgcatt attgctgccc attcgccttt tggcggggta agcattacag tagagttacc
    26221 gctggaacgg gatttacaga gagaagtatg accgagttac caatcgacga aaacacaccg
    26281 cgtattttga tcgtggaaga tgaaccgaag ctggggcagt tgctcattga ttatctgcgt
    26341 gctgcgagct atgcgccgac gcttatcagc cacggcgatc aggtactgcc gtatgtgcgc
    26401 cagacaccac cggatctgat cctgttagat ctgatgctcc caggcaccga tggcctgacg
    26461 ctgtgccggg aaattcgtcg tttttctgac attccgatcg tgatggtgac ggcaaaaatc
    26521 gaagagatcg atcgcctgct ggggctggag attggcgcag atgattatat ctgtaagccg
    26581 tacagcccac gggaagtggt agcgcgcgtc aaaaccattt tgcgccgttg caaaccgcag
    26641 cgcgagttgc agcaacagga tgctgaaagc ccgttgatta tcgacgaagg tcgttttcag
    26701 gcttcatggc gcggtaaaat gcttgacctg acacctgcgg aatttcgtct gttgaaaacg
    26761 ctctctcatg aaccaggaaa agtgttctcc cgcgagcaat tgctcaatca tctttatgac
    26821 gactaccgcg tagtaaccga ccgcaccatt gacagccaca ttaaaaacct gcgccgcaag
    26881 ctggaatctc tcgacgccga acagtcattt atccgcgccg tttacggcgt cggttaccgc
    26941 tgggaagccg acgcctgccg catcgtttag ttttagcgac attattttgt tagccggaga
    27001 tggcgttcag acccgatccg gcaacattat cccacgcatg gtcagcaaac tgacattatc
    27061 tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac agcaccttta
    27121 gatgctgttt tcgatacaca atttcaatca aggagtcatt atggctggtt ggtttgaact
    27181 cagtgagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg gtgagactat
    27241 cctcaccagc gagctgtata cctcaaaagc ctctgcggaa aagggcatcg cgtcggtgcg
    27301 tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg gcaaattcta
    27361 tttcaatctg aaagccgcta atcatcaaat tatcggctcc agccagatgt acgccaccgc
    27421 gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc agacggtgaa
    27481 agacaatacg taattacgat gccgggcgtg ggttgttggc gtccggcttt atatccaaat
    27541 aacgcttagt tattaataat taagagtaac cgccgccatt aaaatagtgg gcactgacga
    27601 gcactcgttg tgctttcttt gtttaccatc ccatacttat cctcctgaac atgttcatgt
    27661 gccaaaatat cttctaatac tttcgtgacg ctatccggtt tatgacatac actgatgtaa
    27721 gatttttact tacaggcggg cgtaaaatgg ccagaacgat gacggttgat ctcggcgatg
    27781 agttgcgtga atttatcgag tccttaattg aatccggcga ctatcgcacc caaagcgaag
    27841 tgatccgtga gtcactgcga ttacttcgag aaaagcaagc agagtctcgc ctgcaagcgt
    27901 tacgtgacat gctcgcagaa gggttgagta gtggtgaagc tcagccgtgg gaaaaggacg
    27961 cgttcttacg gaaggtgaaa gcagggataa ggaaataatg tgcgtattat taaactcatg
    28021 ccaaaagcca atgaggattt agaaggcatc tggtattaca gctatcacca tttcggcgaa
    28081 ccacaagccg acagatatgt ggaacatctt tcagacgttc tccagattct gagtaacaat
    28141 aatataggta cgcccagacc tgaattaggc gaaggtattt ttgttcttcc ttttgaacgc
    28201 cacgtcattt atttcctgca atcaccaggg gaaattatcg tcattcgtat ccttaaccaa
    28261 aaccaggacg ccaccagaca tttacactgg agctaaaccc gccgattgcg ctacaatgcc
    28321 cgcccttaaa gtgggggcac tcccctaacc gcttcatcag gtgaagcgga tctgacctgt
    28381 catcagaacg agagaattat gtttaaaccg gaactccttt ccccggcggg aacgctgaaa
    28441 aatatgcgtt acgctttcgc ttatggcgca gatgctgttt atgcgggcca gccgcgttat
    28501 tccctgcgtg tgcgcaacaa cgaattcaac cacgaaaatc ttcagctcgg catcaatgaa
    28561 gcccacgcgc tggggaaaaa gttttatgtc gtggtcaaca ttgcaccgca caacgccaag
    28621 ctgaaaacct ttatccgtga cctgaaaccg gtggtggaaa tggggccgga tgcgctgatt
    28681 atgtccgatc cagggctgat tatgctggtg cgtgagcact tccctgaaat gccgatccac
    28741 ctttcggtgc aggctaacgc cgtgaactgg gcgacggtga aattctggca gcaaatgggc
    28801 ctgacccgcg tgatcctctc tcgcgagctg tcgctggaag agattgaaga gatccgcaat
    28861 caggtgccgg atatggagat cgagatcttc gttcacggcg cgctgtgcat ggcctactcc
    28921 ggtcgctgcc tgctctctgg ctatatcaac aagcgcgacc cgaaccaggg cacctgcacc
    28981 aacgcctgcc gctgggagta caacgtccag gaagggaaag aagatgatgt tggcaacatc
    29041 gtacacaagt acgagccgat tccggtgcaa aatgttgagc cgacgctggg tatcggcgca
    29101 ccaaccgaca aagtgtttat gatcgaagag gcccagcgtc cgggcgagta tatgaccgcg
    29161 tttgaagatg agcacggcac ttacatcatg aactcgaaag atctgcgcgc catcgcccat
    29221 gtagaacgcc tgaccaaaat gggcgtgcat tcgctgaaaa tcgaaggtcg taccaaatct
    29281 ttctactatt gtgcacgcac cgcacaggtt taccgcaaag ctatcgatga cgccgctgcg
    29341 ggaaaaccgt tcgataccag cctgctggaa actctggaag gtctggcgca tcgtggctat
    29401 accgaaggtt tcctgcgtcg tcatactcac gacgattatc agaactacga atacggttat
    29461 tcagtttctg accgccagca gtttgttggt gagtttaccg gtgagcgcaa gggggacctc
    29521 gcggcggtag cggtgaaaaa taaattctcc gttggcgaca gccttgagct gatgacgccg
    29581 caaggcaaca ttaattttac ccttgagcac atggaaaacg ccaaaggcga agctatgccg
    29641 atagcaccag gcgatggtta tactgtgtgg ctcccggtcc cgcaggatct tgagctcaat
    29701 tacgcgctgc tgatgcgtaa tttctccggg gaaaccacgc gtaatcccca cggtaagtga
    29761 ttaatttcga ttatttttcc cggatggaaa attcttagaa accgatcaca tacagctgca
    29821 tttattaagg ttatcatccg tttcgctgaa aaacataacc cataaaatgc tagctgtacc
    29881 aggaaccacc tccttagcct gtgtaatctc ccttacacgg gcttattttt tacgcgtaat
    29941 acaatgaaat aaaaggattt atttctggtc acgtccacac attgaccaca tcgacaaaaa
    30001 agcccctcga ctgaggggct tcctgtttgt aattacatcc acataatttg ctgccctgac
    30061 ggcaacgggt gcggcctcac ggcgtggact tctcccggct tcacgatgta tcgctgtacc
    30121 gactcataag tgatgaacgt ggcgctgcaa ttcacgttct gacactggtg ataacgctct
    30181 tttgtcgtgt cagtgatata gcggcttgta cgcgcatgtg cggcatgctg gcataaagga
    30241 caatgaaaca tcgcgagcac ctcttccggt tttgttgatg gtgccatttt agttaattta
    30301 tccttataaa acaaacagat aaaataaaag cattactcgt catcttctgt ttcgtactcc
    30361 acatcagaaa gcatgacctc aagctctaag gacgtcgtga agccgctatt attcagaaaa
    30421 tgtgtcacct tagtgattgt ccagtcctgc tcgtctatga cgcgcttaaa gcctgacact
    30481 ttaaccggtg tttccgtgta aatatcagca cgaccagtgg caaggctgat ggagaactcc
    30541 gcaacgcccc gttgcagttt atcccacttc gcctgagcgg cgcgcatggc ctgtgctttc
    30601 gtggcatata ccgtagtcag ggcaaaaacg ttgtcagctt caccggccat gtattcacct
    30661 tcgcgcgctt ccggtacttt tggcgctttc ttctgcgtga ccggtttcgc tttcgggtgc
    30721 tccagtgcgc gcaggtgttt ttctttcttt ttgcgtttca gttttacctt ctgcttttgc
    30781 ggcttcgggt ctttggtgtg taaccacttt gccgttacgc cggtgtaagc tccacggtca
    30841 gcaatcgcaa aatgatgacg gtcgccgtcg ctgcgggtga tggtaatctg cgggattttt
    30901 ttaccgctgg ccgtcacccc ctgccccgcc ttaagaaaca acagttttcc catttttacc
    30961 gacacctcac cgccgttgcg ttcagcaaga cgggtcagga atttcgcatc agactcctgc
    31021 gactggtcga tgtgcgggat tttaattccg gccagtgacg gagcgacact ggcctccagc
    31081 ttgttacggg aggctatcac ctcaacaatc gcaccgagtg tggtgtcatg ccaggagcct
    31141 tcccggcggg aattgagcgt cccgcgaaaa tctgcactcc gggcgcggat ggtaaccaca
    31201 tccggcgcgc cccggtgttc aacctcatca acggtaaatt tccctttgca taccagggca
    31261 aaacctttcc agccgatata caccgtcagg acagcgccac gaaccggcag cccgacctgc
    31321 ccgtcggcat cgttcagttc aatatcaagc tggtcagcct caaagccccg gttatccgtc
    31381 agggtcatgc tcatcagacg gtcgctgata ttgccggtaa tatccctgct gtcgagcatc
    31441 agcatgtaat ccggcgtcag cgtactgcct gcatcaaatg tcagcgcatc cagcattatt
    31501 ccgcccccgt catccccgtg aatttagtcg ccatactgcc agccttaccg atgagcgatt
    31561 ctgcctgttt accgatatcg ccataaagcg cggccagtga ttcatccacg cgggtgagtg
    31621 acagcgtaaa atcaattttc cggggtgtgc cgtctgcaaa gaaaatactc cctgtttcac
    31681 tcaccctgct gatgacatac atgccgtaaa tcatgcctgt gccatccagc aacggccacg
    31741 cccggccttc ctctgccatc agtctgagcg tggtcatcgt cagcttgccg ccggtcagtt
    31801 cgggataaag cacaccggca agcgtcatgt tttcctcacc aacgccgaga aactggtagg
    31861 catcccgttt accgatacgg gaatttgacg gccagcgata atctgattca cgctgcatgg
    31921 tctggtgtgg cagcgtctgg cgcataaaaa caaacatacc taacgcgagc atcatttttc
    31981 gtcacctcct taaccgtcat gcatcatgct ggcacgggcg cgcgcacgtt tatcccgctc
    32041 gtatttttcg agcgcatcct gtaactggcg gtcaagctgt gtccccggcg cagtaccgcc
    32101 cgtcaggttg atgtgatatt cgtttttact ctggtccaca taagagcggc cagccggtgc
    32161 cgtgaccggc tgataagcct gatagcctgc ataagagctg gtcgccggaa tatacccacc
    32221 ggtgccatac gtggcggcat gagtccttgc ggcggtctgg tcaagtgtgt ctgactcttt
    32281 gttgataaca ccgagttttt ccagtaccca gtcaatacca ctgcgcagtt tgttgaacgc
    32341 attaagcggc agcatcagcg cgtcagccag tgcctgcccg aacatgacgc ccgtgtcacg
    32401 gcaacggttc agagtgtcct gggtagcttt gaccggggca atcaggtttt taaaccactg
    32461 ccacgcggcc tgtaactttt cacccagcca gtcaaacacc ggcttaagtg gcgtgaacag
    32521 ttcccccact ggcgcaaatg ctgctttcag cccttcaacc acaccgccaa agaatgcgct
    32581 gacaggctcc cagtatttac ggataagcaa cgccccggcg acaatggcgg ccaccacagc
    32641 cacaatcggc cagctaatcg ccccgatggc cgtcataaca gcactgccaa ccgtcgtgaa
    32701 gattgccccc attgcgcctg ctgccgcgat gatggcatta atgccggtga taaccggcca
    32761 ggctacaaga ccaattgcac cgatgatgcc agtaagcgcc agtgcaccac cgacaataag
    32821 gccgatggtt gacgccagtg atttgttttt ctggatccag ccgtcgagtt ttaacacata
    32881 ctttgtggcc gtctgcgtga gcttacgcag tgcgccttcc tgctggtcaa acaggtcagt
    32941 ccccaccgcc tcataagcgg actgaaactc cttaaagtca ccgccgaggt tgtcctgcat
    33001 gatatttacc agctctgcgg tctttccgtc tgaggcttta aacgcagcgg tcagtttgtc
    33061 cagctttccg gttgaggcgg cagtcatcag cacggcggcg gctgaactgg cctcctcccc
    33121 gaaaatggtt ttcatgtatt cagcctgctg ggcagtaccg agccggtttt tctcaaagct
    33181 ggcctgcatt tctttcagaa tggtaaatac tggtcgggtg tttcccttac tgtctgaggt
    33241 tttcacacca agctctttga gtgcatccca tgcttttccc gtcggtgcct gcaggcggct
    33301 taacacggca cggcttcccg ttcccgccat tgagcctgtg atttttgcat catgcagcgc
    33361 cccgaccatt gcggcggttt cttcaatgct gacaccggca ttttttgcca caggtgcggc
    33421 ataggtcagc gcatcgctca tgccgtcaaa atcggcggcg gttttgttca tcgtcatgga
    33481 gagaacatcc ccgatatgag cgaccttatc gtttgaaagc tgaaaggcgg atttcatccc
    33541 catcagcagg gcggcgtttt cttccatcgt gcgacggttc gccagcgcca tattcagcgt
    33601 gaccggcgtt gccgcctgaa tggcatcaac atctccaccc gctttcgcaa tgataatctg
    33661 cgcaccggct gcatcatccg ccgaggcggc ggtattatcg ccgagctggc gcgcctgctt
    33721 gcggagcgcg gccatttcgg cggaatcttt tgccacaccg agcacggcct gcaattctga
    33781 gtttttctgc gcaaactcat aaccgggcgt cagcaactta actccggcca tcgttcccgc
    33841 cgccgcaatc cccacaccgg cagcgcccac tgaggccata tttccggcca gttccttgcc
    33901 tgcctgataa cgctgtttta ctgcgttaag ttttgcctgt tgcgcactga cacgcgccag
    33961 cgcgtcacgc tgacggttaa gctgtgcggt ggtttcactg atacggtttt tcagtccctg
    34021 ctcatcacgt gcaagattgc gggtattaat tcccacaacg gccagttccc gctgctggcg
    34081 tttaacggaa tccgtcaggc ggttatattt cgcctgtaag tcctccgccg cacgcttcgc
    34141 ggactccagc actttcgcct gagcacgggt cggacgttcg gtgtttttaa actgtgtggc
    34201 aagggcttcg gcttcctgcc gtgccttttc aagtgcatga ccagtcacgg cgagctgtgc
    34261 gctggtcttg cggaatccct caatacggga tgcgtgaccg ttcagctcgc gtagtgattt
    34321 ttgtgtttcc cggatatccc ccgacagcga cttactcgct gtgcggatgg atttaaacgg
    34381 gcgggatgcc tggtcaacag ccctgagcaa tacctgtaat tttacattgt tactcattcg
    34441 tgtttccgct tcgccggagc gccttttcgc gccatgtgat gagttcggtc aggctcatgg
    34501 gatacagttc tgatggcggc cagtgaaata tcactgccac atccgccatc aggtcatcga
    34561 ccgagagatt tttcgggaac gtcactgcac cgagttcggc gacaaaaaac cgaccacctt
    34621 accggccagc gccacaaggt caggcagttc cagtgcggcg acctcctgct cggtcagcat
    34681 cggtgccgtc atgcgcggca gcactttaat cagtgcatcg acttcggagt ttgcgaccgc
    34741 agccagactg acaccgcgca gcgtccccgc actgggtttc atcagcgtga cctgttcgat
    34801 gacctgctca ccacgtttga ccggattgtc cagggtaatc acattttctt tgttcatggt
    34861 tttctcactt cttaatcagg gttaaccggt cagcctggct gaccggatga aaatcacagg
    34921 ccgatattgc ggcggtgttg ctccagccgg tcgacgccgt tcaccttctc aatcatgttg
    34981 atggtgtcga tttcgaccag ctccttaccg tccatcgtca gccggaaata ggtgcagacc
    35041 acggagattt tcgactcggt gtcttctccc tgtttaccct cgccggtgtc gatttctttc
    35101 tgacgtccac gcatgaccac ctcgacggcc accgtttcgc cggtatcgtc gcgctggtaa
    35161 gagcctgcaa aacgaatcgg tacggcatcc acaccggttg cggcgtaaag ctcccagata
    35221 accgaatccg ggaagccacc gagcgaccac tccattgaca gcgcatcgtc atcaaggccg
    35281 aggtctaccg gtgcgctgcc gttcatcccc gcaccgcgat agttttcgag cttacgggtc
    35341 agttttggta gcgtgacgga cttggcgacg ccctgatagc tgtagccgtt cagaaagacg
    35401 ttcattaact tgagtttgcg cggcattgcc atcggtcagg ctccttaatt gctgttaacc
    35461 gaggtgacca gacttgccag gtatcttgaa gtaatacgct ggcgcagggt caggttttcg
    35521 agaggaggca ccggggtata gtcgtagtcg atatacagtt ttccggcctt gagggtttcc
    35581 gcatcgttgg attcttcgct gaaccagcag gtcgcatcca cgatatagcc gtttgttttc
    35641 agctcacgga atttggcatt gatgccgtca acgatgtcgc gaatcagcgt tgcggtgatg
    35701 ggcttgtcca ccgcccacat gtgcgcctca gccatcgtgt cggccagcac ctgcgcggtg
    35761 cgggtgtagt tttcaaagag gaacagcggg tcatcagagc aggtacggtt accccagaag
    35821 cggaaaccgt cgcggcgaat cagcgtagtg acgcctgact cgttaagcag gtcagcatcg
    35881 gtgccggact cctgcaaatc ccagaataca gaggcgctga tgccggtaac accgtttacc
    35941 ccgacattgg acagcgtttt atgccagccc tgctcctggt caattctggc acgcagaccc
    36001 agcgcacggg cggtggcata cgcggtggcg gtggtactgg tgaccgtatc ccatgcgagg
    36061 aaatccggcc agatgaccat cagctcacgc tggctgaaat tctggcggta ggctttcacc
    36121 tcggaaatgg tcttacagcc ccatgcgctg atatacccga aagcgcgcag cttctgacag
    36181 actgatgcca gtgcaacagc cacctctttg gtgtccagtc ccggcacgcc gagaatacgc
    36241 ggtttaacac cggttactga ctccgccgcc agcagggctt tcagtccggt gtactgaccg
    36301 ttttcgtcgg tggtgccgat gatattggaa acggtctgcg caagtttcgt ttcctcgtcg
    36361 tcgccggtgc cgtcttccac gcgcacaaca acggtgaccg gttttgactg gtcggcgatg
    36421 gcctgcagcg acgccgccag cgtgcctttt ttaccggcct ttgcaattgc gctctgcaca
    36481 ttggtaatca gcacaggttt attgagggga aaggtttccg catccgcatc gctggccgtg
    36541 cagaccatgc cgacaatggc agtggatacg gtggaaatga cgcgggtgcc gtcgttaatc
    36601 tccagcacct gcacgccgtg atgatagtca ctcatccgtt taactccgtg gttaatgggt
    36661 gagtggtatt ttcagttgtg ccggagatgt caggctattt gtcccggttg gctaatcgac
    36721 agcacaacca ccggtaaaga aaaggcgggc aatttgcccg cctgtcctga tttgtactca
    36781 ctcattttcc gactgacaat ttacatagcc caaacgctat caaatctgac agtctgcttt
    36841 gggtaaactg ataatgggca gagctttgta tggctaactt cagcatgata ccttacagtc
    36901 aagctgacaa aaggcggaat gattattcgt tgatacacat cctcctgtaa ggaaacaaag
    36961 catgtataaa gaacttatag aacagagact gaatgaggca aaagccgcca gagctgcgct
    37021 gagcaagatt aatggtgtaa ttgtcgaaaa aacacataac cctgaccgcg gactgtatct
    37081 cgttaattat gccaatttac ttatgaacag gcagatagac atcttcgatg atgcgcttct
    37141 cctcctcgaa aacgaacgat accaaagcgc ttgtattgtt agccgaggaa tgattgaaac
    37201 tcatgccttc tcacgactct taaacaagaa agtagagaaa attttgacta cccaaaatgg
    37261 acacgcaagt gtagataacg ctatcgaagt tatattgaac tttacgaata gttctcgatt
    37321 taaaaagtca gagcaagaaa agattcagaa agaagtattt gacccgaacg actacatgtt
    37381 tactgaggag gctaaatatc gttttgaaaa cctacttgct gtcagtatgc atgtgatgac
    37441 tgctttacgc gaactctata aagatgagat gaagcagact aatcagatgg aaagtgattt
    37501 tgaactgact tatgacatac ttagcgaata tgttcatcct tcacaaacga gcatctacca
    37561 ctactacaca cctgaaaccc acttagttcc tacttcggtt ggtaatattc atacttatgt
    37621 gcatgctcag ctacaatgtg tgagagcgct acttttcata ttagaagcaa agagtcaaca
    37681 ttattggtct acacagttag caaatgagat gaccaaacga gctaaagagt aaggcctaag
    37741 ccctgcaatg tgtttatttg aaaaatattt caattttttc tttgtatgct gtaaatgacc
    37801 tgagtccctc tgaccagaga gccttgatgt tatcaatgtc cgctcctggc acagagcgga
    37861 ctgtcagatt aggggttact ctgtgttata gatatgtgag ctcacaccag agcttataca
    37921 gcttattgtg gcatttccgg ccattcagga tttgcaggat ccacacgact gaccagaacg
    37981 ctgtagcgtt cccatgattc cagtcggcta cgctcctcat ctgttgccat gttcagtctg
    38041 accgcgcgct ccagcggcaa aatcacggat tcagcatctg caagaagtct ggctttccga
    38101 atttctgcct gctgctgtaa ttcctctgcc gtataaatgc gtttaatcac tttgccgtcc
    38161 ttaaacatcc agttccctga aatgtccgcc cgtcggttag cagtaatatc cgccacttca
    38221 acaacactta atccatccgg tctgatagct gtcacatcct tttccacata gtggatgata
    38281 ttatctttgt cgtacgctat ttttatcgtg tcatcagcaa aatacttttg ttcttcgtac
    38341 cagttcttac catcttctga aaaaaaccag acaacatcaa aattctttgt caattgatat
    38401 tgttcaaccg tttttggatt acccgccgtt atatttatca aatgctgcat aaattatacc
    38461 tgcgccacgt tataccatgt cccgttaatg tatttctgca ccggtctgta atatacgcca
    38521 ccaatgttat cggcagagtt tgagccggta tcctgaacaa taatgccggt atatacgcaa
    38581 cctgacggag cctgatgcgt ccatgtagta ccattatttg ctggtttata tgtagatgca
    38641 ccgcccagcc gcatgtctct cacataacgt gaatctgatt cagctttggt gtatgcacca
    38701 acatctcccg ctgagggttt atgagcagtt gtatatagtt gcgcccatcc agaccagtta
    38761 gcatctgtgg tatctcgtcg ggagcgaata tatgccgggg catgagcacc gctggttcca
    38821 ctccagccaa taagtaactc accctctcct actgcggtta cgccagtgag atgaagcaca
    38881 ttaccatagg ctgttgggta gccattgttg tatgcctcat acatctgaat acctggtgtc
    38941 ccctttacgc tcccacctaa tgcagtaaca cggtttctgg ataccagtgt attaatattt
    39001 atatcagcag agccatcaaa tctgacacca ttaatatttc ttgcgttcgc taacttcgtt
    39061 gctgttgcag catttcccga aaggctggaa ataaatggat gtgagcagta ataaccgcgc
    39121 ccatttttaa aatccagaat agcctgtgcg ttcgtgcttt ctgtagcagg attagttgcc
    39181 ccccacttat atgtcgtttg accgacgaca taatcctctg tcggaacaat aaccgttagc
    39241 ccttcctctg caagaatttg cacagggaaa gctctggctt caacataaaa aacactacat
    39301 acatcatcat ctttcaggct tgtaacaatg gaatggattg aacgctcatt agtctgatac
    39361 gtccagaaat aacctgccgc atatgaacca cgatcagtcc agcctccggg cataaccatg
    39421 ccattaaact cgcagttatt cattacataa tcgccgttat aacaaccagt ggaaataacg
    39481 acgcgggatg ccatttctcc tgaaaggctg gcagcacggc gaaagataac gggataccac
    39541 ttcccggcaa cgacatttgc aggggctgca aacgaatact ttcgcattcc ctttttctta
    39601 tccacttcac ctttgctgta aacattaatg ttactcagga agcgtccttt atcaggaata
    39661 tccgcaccgt tctgatcttt ctgaagacgt ttttcagcat tgtcataggc agacttcacc
    39721 gcttttggtg ttgcggcctg cgtttcagaa tcactgttgg tggcgctact gagctggaca
    39781 aggcctttcc gcgccgtggt ggcatcctgt gcagtgtatt tcccgttagc aaggtcatac
    39841 gcggccttaa ccgcctttgg cgttgccgca agcgtttcag aatcgctgtt ggtggcacta
    39901 ctgagctgaa caaggccttt tcgcgcggtg gtggcgtcct gcgcggtgta tttgccatta
    39961 gcaaggtcat atgctgcctt aaccgctttt ggtgttgcgg cgagcgtttc agacgtgctg
    40021 ttggtggcac tactgagttg gacaagacct tttcgcgctg tggtggcatc ctgcgcagta
    40081 tatttcccgt tagcaaggtc ataggcggtc tttactgctt tcggcgttgc ggccagtgtt
    40141 tcagacgtgc tgttggtcgc gctgcttaac tgagtaaaac cttttgcggt cagagaagcg
    40201 tccgggtgac gtcgtgactg ttcgtgctct gcaattttgt catcaacgta atcctgcgtc
    40261 gccatcaccg ttgtggtgtc aatggtcagc tccactgagg ccacactgct gacgatgatg
    40321 accatgcggc aggtctgcga acgccctgag ccttcggcaa gggctggctt ataactttcg
    40381 gccatgttcg ccacggcaat tagcgttccc gcatcatcgt acaggccaag ttcacgcatc
    40441 cagaaaccgc ccacctccgg cggaataacc agctctgcga taatataatt actgtttcgt
    40501 ttgtcctggc tgattttgtt cagcgcatgt cgccagactt cgtggataag cccggtctgt
    40561 ccggcatccg ggacaggcaa tttaccaccg ccatccccga cggccatcgt ggtaatgttg
    40621 accttccgcc ctcccggtgc ggttgccgct gccagctttg ctgcaccggc agtggtgata
    40681 acggttctga attttgtgct cattattcct cacttatccg gggtaaaccg taattacatc
    40741 gccgtcgtaa gccacaccac cggcgaacag gtagccggga atgtcccggg taatgttcag
    40801 gccaataaga tgacggcttg caggtttggc atcagcaata agccgttcca tttcctgata
    40861 cattgcctct gtgatgccgc tttccagtac accaatatca agccggaagg tgcctggcgg
    40921 gtcactggtt tcccaccact ccgtcacgtt gatgagatag ccgagcggct ccaccacacg
    40981 ccggattgca cctattgtgc ctttatgaca gtggatgaaa tacgcatcgc ggataacggc
    41041 gcgttttgtc gcttccggcc acttttcatc ccacctgtcg accgaaaacg cccacgccag
    41101 ccacggcagc agatttgccg gacaggtgtc cgggttccac agctcacgaa tactgaccgg
    41161 cgttttttca atttccgcac aggcttttgc ggcggcgacc tcaagcggtg atgagccggt
    41221 cggcagcagt cgcgaatcac tcatccgagc ctccggtcac gacgcggtat tcggtacaga
    41281 aagacgcctg cgtactgttg agcacgatgt cggccagtgg tgcagccagt tcgacacgct
    41341 gcacaccttc cacatgcaaa gcggcataaa tggcagacag acggatgtcg cgccccagcc
    41401 ggtgctgtgc cgtgatatac gcttccagct ttttcacagc ggcagcgcgg atgggttcgc
    41461 tttcgggacc agggtaaagg taaagcgtgg cgtttatctg gtattcaacg atggcggcag
    41521 actgcacggt cacgcggtcg gccaccggcc tgacgtcctc gccattaagg gcgttacgca
    41581 ccacagccag caggtcttcg gatgcgacgc cgttattttc acgtgacagc acagagatgg
    41641 tgacacaggc aggagacgga ctggtgacag aaatatccgc gacacgcccg tcagcactgc
    41701 gaccatgata ctgataggct cccactgacc cggcgacgct taagccctca aaagcctgct
    41761 gaatacgcag acgataatcg gtgtcagatt ccatcactgc cggtgtcggc ggaatggtcg
    41821 aatcatctgc cggggtgata gtcaggcgcg tggtgttgta attggcacca atcacatcaa
    41881 ggtcattacc ggcggcacag gccagcatca ccgcccgtgc ggcctcattc acacgctgac
    41941 gccagataag ctcacgataa gcattttcct ccagcagttt gacgagaggc tcagattcca
    42001 gcgtcagggt acgggcgacc gcctcctgct ggtcttccgg gtaaagggaa atcagtgtcg
    42061 ccttgcgttc agcgagaatg ctttcaaagt ccagctcctc gaccacatct ggtgcgggta
    42121 gctggttcag gtcgataatc ggcatggttt caactcacag ggatggttaa tgaaagtggc
    42181 tggccggtgt cgttgtgctg gccggttaac gtgaccgtca ttcgcccgtc aaaactgcgc
    42241 gccgtggtga cggatgacag ggtgacgcgg ggttcccatt tcagcaccgc catgtaacag
    42301 gcgaccttaa tctgcaactc aagcgccggg gtctgcggct ggtcaatcat tgacgccagc
    42361 aacgagccgt aatcacgacg catcacccgt gagccgaccg gtgtgcgcag gatatcgccg
    42421 atactctggc tgatatgctc aaggtcagtg acagtcaggc catcactgcg attcattccg
    42481 agataacgtg cagtcataga ggtcccccgg ttgtgccgcc gctgtcgccg gggtgtttat
    42541 gggtatgcag taccttaccg tttgatgaga gttcaccgcc ggtgtgttca atgttgccgc
    42601 gcatcgtccc gcccttctgc acttccagcg tgccggtaat cagcctgttg gtgcagacca
    42661 cctccggtgt gtccagggta acgcgggttg atgctttcac cgtgaccacc ggcaccgtgg
    42721 cagtaacaga atcagaagcc gtcacgctgg ccgttttaat tccgcttacc gtgagtgcac
    42781 tggtttcggg ttcgtactca atcaccgccc cgtcagggaa acggatatgc agggcatccg
    42841 ccgacgcaga cggcgcgggg ttatcgccgg aataaatccc cggcagaacg aacgcggtgt
    42901 cgagttcgcc gcccacggac agaatcagca cctgttcccc ctcggaaggt gcccaccacg
    42961 tgcgcgaacg accggcgcga tgggtcagcc actgaagcca gtcggtgcac atgccgccag
    43021 tctgcacacg gcagcgaccg gcgttaaggt cggtttcgac gacaagaccg gtgcggatca
    43081 tattgcgcag tgcgcgcgcg agttcctgaa tatttgcgag agtgttcata acgggaagga
    43141 tgccgccggg tcataccggc ggcaatgtga cgatgaggtg tcgggaatgg cacaactaac
    43201 ggtcgaggtg agccaggata atctcttcaa tcatctgcac atcctcaccg gtaaagccga
    43261 gcagaggacg cgccggataa tcaattttct taccgtcttt ccggttttct tccgacagac
    43321 cgaactgatg cacactggcg attttcggtg acttcccgcc gtaaaactcc attgatgcct
    43381 gctccgggct ggcgcggata tgcaaaaaac gactggtgat aagtttcgca aacatttttc
    43441 gcttaacacg accagtcttt tttctggcgc tctgctgctg gcgtggcacg tagggtgtgc
    43501 cgtccggggc tttctgtgcc atcacccgac gctgctgact ctgccgcagg cgcttcgcca
    43561 gttcggcgct cagtctccga cgccctgacg gtgacaacga ttcaatcagt ccggtcagcc
    43621 ggtcttcaaa acgcttaaac tcattcatcc cacttactca ccagttcgcc attgatatac
    43681 agctccatcg ggcgggtgac cggctccggc ggcagaggtt ccgggatatt cttcacatgc
    43741 agcgcgccgt ccacctcact gaccagcgtg cgctcggtca gcatcaggct gatgctgata
    43801 tcaaagctgc tgtcattgtt gatgtctgca taaaacgtga agcccttttt ctggccttcg
    43861 tcggtggtca tgatgtcggg ctgattttcc cgcagccacg ccagcaccgg cacgatgagc
    43921 aggtcaaaat caccggtaaa atcggtcaca atgacattga gcgtgtaacg cttttcgaat
    43981 gacagcgacg tcgccagtgt ggaggcaata ctcccgttat ccacgaatat ccgaagcata
    44041 tcggggttag ttttcagcac cgtgacggca tcagtcagcg ccctgcgcag gctgtcgggt
    44101 ttgagcatcg ttttcgtcct gacagtgttt aatcattttt acctggctgg cacagcgtgc
    44161 cagcgcgttc tcaagctgcc ggatatcggc acttaaatcg ccgttcgtct gcgggtcact
    44221 gcccggcatc gggcaaagac tcactttcgg gcaggcgttg tggacaatca ctggcgtcgg
    44281 tgcaggcggg gcgctggtgc aaccggcgca cagcatcagg caggtcagcg ccataccagc
    44341 ggcggaaatc ttcgttttca ttcagtaacc tcgtgatggc tttctcgcgc tgtgcttcac
    44401 gcttcgcggc attttccagt tcctgacgca gtgccacctg cgccagctcg tttttgtctg
    44461 ccctggtgag cgcaacatga agctgatttt tcagcatggt gatggtcgtc tgctggcgac
    44521 gttattcgcc ctgtccagcg aggcgcgcag gctgtcattt ttgtgtttca ccagaaacag
    44581 accggccacc gccagcgata acaacacaac cagcacagtc atcagccttg acatggttcc
    44641 cgcccctcaa tacgctgacg gcaggccgtg cgtatcagcc ggaaaaacag cgacgtcatg
    44701 agataaatca gcgcggtaaa aatccacccg gcagcaatca gcgagacaaa cgtcgccacc
    44761 atcaccacca gagccgccgc ccgtctgcgc cacggcaccg gctgtaaaaa cagcgacgtg
    44821 acaatcttca cggccagcga ttccggtggc agctcccgcc cgtagcgttc cagcacatac
    44881 tcagtggcat acacgccgac accaccggca accatacaga taaccgtcgc cagaatcgcc
    44941 caggcggcga caaaactgac ggccacgctc tgcgggtaaa tcagggacag tgccagcatc
    45001 agcgccagcg acacgttcag catcagtgaa agggataatt tcttcatggt gtttactccg
    45061 tttaagccgg tacgccgcca gcggtacgcc agacggtgac cagtttttcc agtgaatgct
    45121 cacgctgacc gtaaccggca cccggcaggg acgcccagat attgcgacag cgtgaaatgg
    45181 cgcgctcaat gcgtcccgcc cggatgtcat ccagcgcacc gcgttcgcgg atcaactgaa
    45241 tggcgagcct gtcctgtgac aacggactga aatcaggcag ggcaagctgt ttgcggtagt
    45301 gcggccagaa caggtaaagc tgctgatagc gaccggaggc cgtggatttt tcaccgcgac
    45361 ggttaaacac cttcgccggt cggccatgtg cgaacgggtg gtcactgtag tcggtgaaaa
    45421 tttccggctt cccgtccagt ccggtgacta tcacgtcata gccccggttt ttcgtcagcg
    45481 ggtgattcgc cgtcccttcg gacactgcca gcatgtcgag aaaggccgcg atattctgat
    45541 gcgtgttaat taccggcatt actgtttccc cctgccctta aaacggcgct gaatggcaat
    45601 ctcaatcacc tgataaccgg cgatacccag catggagccg atgccgcaca ccgcaggcag
    45661 tgacaggtca ggaaactgca ccagaacaac accggcaacc atcgagacaa aaccaccgag
    45721 caacatgcgc ccgataaaca gacgcggggt gatgggttca ccaccggcaa gcaccttgcc
    45781 gacaacaatc agcaccccaa tcatgaaaag cgacaggacg cttttttctt ctgctgtcat
    45841 gcgttactcc cacagattga cagtttcagc cacgggcgcg gtctgaacgt cgggcagttc
    45901 gacggcggtg ccgtgtggca gcaccgcacc cagttcagcc agtcccggat ttgtggcgag
    45961 cacggtctca accacaccct cagtgcgccc gtaataccgg acacagatgg cgtcgagcgt
    46021 gtcgccctgt agcgcaaagg tcttcatcag atttgactca cgatgcagcg cggcttgtcc
    46081 tggatgcgcg ccactgccca gcgcatatcc cgccacagct catcaatggt gctgtcaatg
    46141 ctgtcggcct tcttgtcgcc tttcgcactg gcatccacac cgcgataacg ttcataaagt
    46201 gacgcggtcg ccatcgcaca cacggcgcgc tcgtagtaaa aaactttgat gctttcaccg
    46261 tcgatgtcgt ccgccgggac gtccgccaga cgcgtaaaac cggcggcaat tttctgttcg
    46321 cggtactcgt acagctccgc attcgtctcc gccatgcctg acttgatggc ctcacgcaga
    46381 cgggcggggg cgacggtctg ctcaaggcgc atacgttccc ggacgcgctt cgggtcgata
    46441 tcgggaaaaa agaacgtgtt tttaatcacc ggctcgtcgc ctgccggttg cgggatgacc
    46501 accgtaccct caccggatac aggagcctcc tttcgcggaa taatcagcgt catcatgact
    46561 acctctgaaa agtcgggcgg tggacgccgg tgcagtgtca ggtgattcac cctcactgac
    46621 cggcgtgccg ccctggcgcg gggcgcattc ggttgttaac tggctttctt tttcgggcgt
    46681 ccacgttttg ccggtgtcac gctccgggtc ttacgcgggg cgcgggtggc cgcttttggc
    46741 tgcggctccg gcttcggttt cagctcccgc tccagtcgtt caatctcttt tttgacgcct
    46801 gcctgacagt cgagttgtgt cgcacgttgc aggtgcgcca gcgcacctgc ggcatcacca
    46861 gcgtcacgca gaaacagacc ggtgattttg tgcagctttg cgcgcacttc atcaggcatg
    46921 tctgccgtgg cagtcagttc aagggtgtcc gtcagcaggc gggtatccac agactcaccg
    46981 gcagcgtgag cacgcatggc cgcgagcgcc acctcctcgg tgaacatgta cggcggggtg
    47041 cggcggtgtt tacccggcat ggtcagaccg tacttcaggg cataacgggc aatctccagc
    47101 gcaccggcaa tatcgccggt atccagacgc cacagcatga ccgtcatcag aatgtcatcc
    47161 tgtgcacctt tgccctgctc cagcacgccg ttcacccacg gcaaccagaa cggcagcagt
    47221 tcgcgttttt tcgcggcctt cagctctttt gaataaatcg ctttcagtgt gcgctggtct
    47281 gcggccagct tgaccagcat ctgctcatag acagttgcat gtcgcagcgg ggcggcttcc
    47341 cgctgcgcgg tcatcgctgc cgagacccgc atcatgtggc gctgtgcggg actcgtcatc
    47401 ggttacgctc ccggctctgc ggtcgcttta gccggtgtgg agaagtcacc gaccttgatt
    47461 ttttccacca gacaaccggc ggcgtagtct tccaccacgt aatcaatgtt cattgactcg
    47521 tagttctcca cgcggtcgag tttcgggttt tcctcaatca cgcggcgatg gctgtcatcc
    47581 atgtagtaga tggacaggtt ttccagcttc gtgatgagca tcgcatccgc cgggaagtac
    47641 gggacgcgta ccgctggcag gttgccgatg cgtttctggc tgatgatgac gtcagcggcc
    47701 agcatttcgc tgttgtcctg ctccttgttg acgatgggaa aatacttgtc cgccagtagc
    47761 tgacgtccca caatcaccac aaggtcaggg tcttcctgat accacggctc aatcaggttg
    47821 ttggtcgcat ccatcaccag tgcatcaagg ctggcataat caccgccctt acccacgcgg
    47881 atgacctcag aggtggtgtg cccttcctcg tcagtgacct tgctcatcac gcgcgccggg
    47941 gcttcattac ggtatttctg cagccagccg accgccacat cctgcagcat cggattgctg
    48001 ttgcggtcag aggtttcggc acgcttcacg ccgttaaaac cggccatgat gaaatcaagg
    48061 gactggcgtt tgataatggc gttacggata cggagctgga aatcctgata acgcgcccac
    48121 aggtccagcg ttttgtagcg gatataaaaa tcgaagttaa tctggtcgca ttcgtacttg
    48181 tttgacgcca gcttcgagaa gtccttcggc tgacgctcgg tgccaccggc ggtgtcggtg
    48241 gtgctggcga tggagccggt gacaccgatg ccaatttttt cccctttcat ttcgctgacc
    48301 ggcacaatgt tgatgcgggt cagaaagtca gaggactcct gcatggtgtt catcagggtc
    48361 tgggtgaccg acggttcaac ggtgaatttt ttcgacacat caccggcgtc gatgccgttc
    48421 agttcggcaa cacgggacag gtaggcatta aatttaaagc gggtttcctg gcgcatagtt
    48481 tttcctgaaa ttaagggtta atcgtgaagg ttttcccgga ctgactgacg ccggtcagca
    48541 gttcgtcagc agggcatcac cgccaccgcc ggtggcttta ctgcggcgct gctgggtcag
    48601 actttcggtg ctgtcgagac tgtttttcag gcgggtgaat gcctggctgg tttcatccgc
    48661 cctgtcagtc acatcctgct taagtgcgga aaaggcggtt tccatctcag cgagtcgctg
    48721 ctcagtggca ctcagttttt cctgcacatg ttcagcaaca gcggtcaccg cttcatgcac
    48781 atcattcaga cgggcatcat cgctggcctg tttgcggcca aaaatggatt tcaccttttc
    48841 ggtcagggcg gtgaacacgg tttcaggcag gtcttcaaat tccagctcaa caggcgttgc
    48901 cactgaaatc aggttttcag ggcttaattt gaagcggttc agggggttgt gttttgccgt
    48961 gcggcagaat tccaggtatt ccgtgccgag gcttgccggg tcatcggtga cggccagacc
    49021 caccagataa catttgccgg tgttggcaaa gttcggctga atttccattg aggtgtagac
    49081 cttctgcgcg gccttgttca tcgcgataag gtcatcggtc ggggtgattt tcgcaaacag
    49141 cgcccatttg cctttcagcg ccgaatcatc gtcaatcttt tcggccttca gttcggccac
    49201 atcgccataa cgcttaaaaa taccgtcagg caggatgccg cgcagatgtt ccaggttaat
    49261 gcggcaacca tagactcgcg ggtcaaaggt ttcggccatt tcctgaatat cctgcgcact
    49321 gatgacacgc ccgtcacagg tgtcaccctc aacgccgata cgaaagaatt ttgagacttt
    49381 ttttgccatt gtcaggagtc ctgaatagtg attagaggag tcacatgtcg gcatcagttt
    49441 cccgacgatg cgcatcctcc gccatcagtc ccggatggct tatcactgac acaacagcac
    49501 cttagcgaat cgcggggcgc gactcagtag ccttgccgtg tattcatcac ggcgaggtat
    49561 tcatgaccat caccacagac accactcttt tacacgaccc gcgtcgtcag gcggcgctgc
    49621 tgtactggca ggggttttcc gtgccgcaga ttgccgccat gttgcagatg aaacgcccga
    49681 cggtgcagag ctggaaacag cgcgacggct gggacagtgt tgcccccatc agccgtgtcg
    49741 aaatgagtct ggaagcgcgg ctgacccagc tcatcatcaa accgcagaaa accggcggtg
    49801 acttcaagga aattgacctg ctgggacgtc agattgaacg actggcacgg gtaaaccgtt
    49861 acagtcagac cggcaacgag gcagacctta atccgaacgt cgctaaccgc aacaaaggcg
    49921 ggcggcgcaa accgaaaaag aattttttca gtgacgaggc catcgaaaag ctggagcaga
    49981 ttttctttga gcagtctttc gactatcagt tgcactggta tcgcgccggg cttgagcacc
    50041 gcatccgcga tatcctgaaa tcccgccaga ttggcgcgac gttttatttt tcccgcgagg
    50101 cgctgctgcg cgccctgaaa accggccata accagatttt tctgtcggcc agtaaaacgc
    50161 aggcgtatgt gttccgtgaa tacatcatcg cctttgcccg tctggttgac gttgacctga
    50221 ccggtgaccc gattgtcctg ggcaataacg gcgcaaaact gatttttctc ggcaccaact
    50281 ccaacaccgc acagagccat aacggcgacc tgtacgtcga tgagattttc tggatcccga
    50341 attttcaggt actgcgtaag gtggcatcag gtatggcctc acagagccac ctgcgctcga
    50401 cctatttctc caccccgtcc acgctggcgc acgacgccta cccgttctgg tcgggtgaac
    50461 tgttcaaccg gggacgcgcc agcgccgccg aacgcgtgga aatcgacgtc agtcataacg
    50521 cccttgccgg aggtcttctc tgtgcggacg gccagtggcg gcagattgtc accattgagg
    50581 acgccctgaa aggcggctgc acgctgttcg acattgagca gctcaaacgc gaaaacagcg
    50641 ccgacgattt taaaaacctg ttcatgtgtg aatttgttga cgacaaggca tcggtgttcc
    50701 cgttcgagga gctgcaacgc tgcatggtcg acacgctgga agaatgggaa gactatgcac
    50761 cctttgccgc caatccgttc ggctcccgcc cggtatggat tggttacgac ccgtcacacc
    50821 gtggcgacag tgccggatgc gtggtactgg caccgccggt ggtggccggt ggcaaattca
    50881 gaatacttga gcgtcaccag tggaaaggca tggactttgc cacccaggct gaatccatcc
    50941 gcaaactcac cgaaaaatat aacgtcgaat acatcggtat tgatgccacc ggcctcggtg
    51001 tcggcgtgtt ccagctcgtg cgctcgttct atcctgccgc gcgcgatatc cgctacacac
    51061 cggaaatgaa aaccgcaatg gtgctcaagg caaaagacgt tatccgccgt ggctgtctgg
    51121 aatatgacgt cagcgccacc gacatcacca gctcgtttat ggctatccgc aagaccatga
    51181 ccagcagcgg acgcagcgcc acctatgagg ccagccgcag cgaggaagcc agccacgccg
    51241 acctcgcctg ggcgaccatg cacgccctgt taaatgagcc actcaccgcc ggtatcagca
    51301 ccccgctgac atccaccatt ctggagtttt actgatgagc aagaaaaaag ggaaaacacc
    51361 gcaacctgcg gcaaaaaaaa tgaccgccag cgccccgaaa atggaggcat tcacctttgg
    51421 tgagccggtg ccggtactcg accgccgtga cattctggat tacgtcgagt gcatcagtaa
    51481 cggcagatgg tatgagccac cggtcagctt taccggtctg gcaaaaagtc tgcgtgctgc
    51541 cgtgcatcac agctcaccga tttacgttaa acgcaatatt ctggcctcga catttatccc
    51601 gcatccgtgg ctttcgcaac aggatttcag ccgctttgtg ctggattttc tggtgttcgg
    51661 taatgcgttt ctggaaaagc gttacagcac caccggtaag gtcatcagac tggaaacctc
    51721 accggcaaaa tatacccgcc gtggcgtgga ggaggatgtt tactggtggg tgccgtcctt
    51781 caacgagccg acagccttca cgcccggctc cgtgtttcac ctgctggagc cggatatcaa
    51841 tcaggagctg tacggcctgc cggaatatct cagcgccctt aattctgcct ggctgaatga
    51901 atcagccaca ctgttccgcc gcaagtatta cgaaaacggc gctcatgccg gatacatcat
    51961 gtacgtcact gatgccgtac aggatcgcaa cgatatcgaa atgctccgcg aaaacatggt
    52021 gaagtcgaaa ggccgcaaca actttaaaaa tctgtttctc tatgccccac aggggaaagc
    52081 cgacggcatc aaaattatcc cgctcagtga agtggcaacg aaggacgatt tttttaatat
    52141 caaaaaagcc agcgccgctg acctgctgga cgcgcaccgc atcccctttc agttgatggg
    52201 cggcaagccg gagaacgtcg ggtcgctggg tgatattgag aaagtggcaa aggtctttgt
    52261 ccgcaatgag cttatcccgc tacaggacag gatccgcgag ataaacggct ggctcggtca
    52321 ggaggtcatc cgctttaaaa actactcact agacactgac aacggctgaa catcgccgcc
    52381 tgcgggcggc ttttttacac cccgtcatca cgccctcaca cgctcaccac cgcacaaaac
    52441 accccgcaga cacaccaacg cccagcaggc caagtaaacg ccttcacgac gcgctcagac
    52501 gctgaaaaaa taaaatcagc accaccgcca gcgcgcagtg ctttccccgc ctcgcccgcc
    52561 cgcttcatga gccggtttta atgcagatgc atgtgaacac tagagattga ctggataaga
    52621 cctaaggttc attaattttt tgttttttta catgcatttt gatgcaagaa catgccctct
    52681 aaagtcttag tttaatgtgc tacactttta tacaaaccta tgataaaagc gataaaaaat
    52741 cttccatttt ggcgaacatt atgacggata ttgaaacagc tcgatggaac acagaaagtg
    52801 ccgctttgtt ggcactatca gaaatacatg gcgtgagtta ttggactcta tataaagttg
    52861 ctcaaaaggg aatcagattt agagacattg ttaccagcca aacattagcc aactttgagt
    52921 atctactcgg agtaaaactt catcgccaac cttattattt gaatgaaagc aattggtctg
    52981 tttttcggga ccgcatgatt tctacggcta aaatcttact cacacattat cataatagtg
    53041 gttacaaaat tatccaccat ggttctccaa actatccaga caagttgaat gacctgtctg
    53101 aaccgccttt ctggttgttt gctcaaggcg atgtatcttt gctagataaa aagtgtgttg
    53161 gagttgtcgg cactagaaac ccaaccgcgc ttggaattta tttaactcaa gcagtaatct
    53221 cacaatttat agattcagat tattcaactg taagcggttt agcatatggt atagatcaat
    53281 ctgcacatga agcatcccta ttatttaaaa taccgacaat tgctgtgcta ggtacaggtg
    53341 taaactcaaa ttaccccaaa aatagtggcg agatgagagg ccatattgtt aataatggcg
    53401 gactgattct tactgagtat ttaccaaacc aaaagccatc acaggaaaac tttgttcgta
    53461 gaaatagaat tcaagcagct ttaagtgatg tattaatccc agttgaatgg ggcctaaaaa
    53521 gtgggacatc tcatactgtg agatatgcag ctcaattaaa aagagcgata ctttgtcctt
    53581 tacttagggg aaccacacct caagaagaaa ttaaacatgc cttgtcggag tactcagcta
    53641 cgataatgaa cattccacta tccgatttta aagacgttca aaacttaatt aaatcagcat
    53701 ccggtgtaaa aacacagcaa ctttctcttc tgggagatga atagtatgta tttaaaggca
    53761 gttatatttt caatcgcaga tgttgtatta ccattaacat ctaacactca acctcaggaa
    53821 ctaaaatcta gaatcgactc ggaattgcgt aagttgtttg cgtttttgtc atcaaaaggg
    53881 attaaagtta tcttcttgac aaataaaaat agaaacgtgg aaacacatga cggggtcgta
    53941 acactagacg aatacctaaa aaggaaattc ccagaatcaa ttcacttttg ccatgaactc
    54001 gacaacaaca taccagcaaa acaaacaggg aaggccatag atttcatcat ggctgcatta
    54061 gagttgaaac gaaacgaaat gatctatgta gggcgttctc gggaagactt acaagcagct
    54121 accaacggta acaccctatt tattaatgca acctggtatg aaccggttac tgagtacggt
    54181 tttcaatttt cagaacccaa agagatcgct agatttattg atgttttttg cttaagagaa
    54241 cagttgtggg gatggcaagg gcattttaat gaagatgttc actactacgc cttggcacca
    54301 tttagcacat atgtcactga atttacaatg tattcagcca atgcaagaga cctcgccaag
    54361 ctatcggttg gaagccccga tttttggata agatatcttg gtgccagcat ttatttctct
    54421 ggcttaagtg agggagcaag ttttataacc acctacgttg gacataacgc tgaaaaccct
    54481 tataaactag cagatattat ggagcatgac ctaaaaggtt tggcggtctc attcaaagga
    54541 aagtatttaa aagatctatt cttacgacat acaacagcca ttaagtcaca gcaagcccga
    54601 atagctaaac aagaagtaaa catcacatct caaataaata ccgttcattt aaaccctgcc
    54661 cctattaaaa atatcaacac cggcaaaaga tataccaacc cgccgaactt gaaaggtaag
    54721 aaaatattag ttattgatga tttttgcact gaaggaaatg ctcatgaaac agccaggatg
    54781 tatctaaaag ctgcaggcgc aaaagtaata aatatctcgt ggttaaaaac catcaatcgt
    54841 gacgtttcta tctgtgaacc aactcgcaag atacggccat gggacgccaa tacacttgat
    54901 attgatgata ttaactatgt cggcacgatt ggttatgctg aaaatgtaac tcacggtagc
    54961 gcgcctcaag tgttatcaga aaaaattcga caatatgata actgggactg gccacaataa
    55021 atcaccccca tcaatcaaag tgccccctaa taatgggagg cactttaaaa tttaagaaaa
    55081 caatgacatt aagcaatatt taaccattac ttaaaacatt acaattattg ttttttaaat
    55141 ttgctatttt ccaatccatc aatcagacct gtatttattc ataatctaat agccttacca
    55201 ctcatcaatc accggatatt tgaatttttt cccgtcataa tttacggttg ccccacgcgt
    55261 cagtgcctca agctcccatc gctgcggcct gataccgttc tgagcaaggt caacgcggat
    55321 acgggtaatt tgaaatcgtt ccgaccgggt cagcctggct gatggcgcta tttcatgtgg
    55381 ttttaacggg cttccgtttc tttgctgacg gtttggtgtt ctcaggtcgt gttttaatgc
    55441 gcccctgagc accctcacaa cctccgggtc actccattcg ataacaccgt catcaaccag
    55501 attaagcaca gctgcggcgt gctcagaagg tgtgggagcc ggtaacgaag tatcaccacc
    55561 ggtgagcttt ccacagttat tgacaggact ccgaggcgcg gcgatgccgc tttttaaagt
    55621 caaaggctca acgaccggca ctttcggaac aatgcgccag ttcgtcgttc tggtgatatg
    55681 aatatgacgc gcgccgagat gcggcgcgta aatgccgacc actctctcga cttcttcctc
    55741 gtactcgtta acgtcatccg acgggctacg ggcgaccctg acagtctgac aatcgcgcgg
    55801 gacatttgcc ccgccctgcg cgctgatata caacgcaaaa tcaccactgt ctgcagcggc
    55861 gcgtgcagcc tcgacgcgct cgtcaaactc atcagcaatg ctgacgccac gaggcaattt
    55921 gcgtagttca cggtaagccc ccattgtcgg cagaccaacc gttttaaatt gcggaatgcg
    55981 ccacgttgac gcccatgcgg taacagccgc agctgtgtct ttcagcggcc taccggtatc
    56041 attatcgagc tgaccatcca gtgcatagcc gtcgatgttt tttgagatgt atttcgcgat
    56101 atatcccgca gcaccgcccc ggttaaggtg ttttgcctga aaacggtttc gcgcggctcc
    56161 tctttcgtcg ccatcctctt tgagcgcata gcgacgcatg atttcgataa tctggttacg
    56221 ctggcgtgga tgacaaaaaa gcatcatatg ccagtgcggc gttccgtcgt ggtgtggctc
    56281 gacgactcgc aaaccgtaga cctgtaaatc attatccttg aatgccgtgc gcatcaggct
    56341 ccagatacgg cagagataac gctgcgcatc ctttggatta aatgcctcat cgttccagcc
    56401 gtgattaagc tgaacggttt tactttcgcc ttttccgacc tgacgtgtcg ggtgatactt
    56461 tgacggcgcg gtcagcgtga taaacatccc cacatcaccc tctgcggcgg cgtaacgctc
    56521 aataccggca atggtgttca tcagctccat ccggcgaatt tcaggattag aaatactgcc
    56581 catcacctta ctgataaggt cgatgcgctc gccggtttcc ctgttttcga ggtcacacga
    56641 tttaagaaat tccagatttg cctggcggcg cgcacgcaca tcacgaatgg catgtttact
    56701 ggcataagga gaacggtctt tattgacctc cccgacagca atcagtaacg cctcatgcca
    56761 gcgcatacgc tgacctttaa gctgatgagt ccaccactca tcgttaaaca gacgggcaat
    56821 ggcagaatat gcctgcctcg tggtcatctg tcctttacgg tattttttcc agtaaagcgg
    56881 ggaaatattg aaagcacgtg cagcgccagc aacatgacca tacagatgcg cctgcgcctc
    56941 atccgtaaac agcgattctt tttcgccatg cgcatccacc caggcatcgc tgagttcctc
    57001 atacatcatg aaaagctgcg atgagatacg ggcggcaaac tttttcagct ccttgtcatt
    57061 cattcccggc aggcgcgcat agtggtcacg ctctgccaga aacagcaacg acgcgtcggt
    57121 gttcatttca tggcgctgat tcacacgctc aatgcgcggc cataaacgac gctgaaaagt
    57181 agatgtgagg aaataaaacc cgtgcaccgg gcttttattg cgccggatgt agtcatagcg
    57241 tgaagtaaac agcgagcgca aaaagtaagg caggcgatta atcgtggata aaacaccttg
    57301 cacctgacgc atctcgtcac gtgtaagggg tctttcgcgc ccgacagcct cgcgtggcgc
    57361 gttccatgca taagcaccgg taaacgtctt accggtgcct gcggcaaatg ctgacggagg
    57421 gacaaaacgc ccggaggctt taacggccat atgagccaaa agcctctgaa caacgcttgc
    57481 tgagttgctc aacctgcgcg tttaaatcag caaaagactt tgcgcttccg gtcagaatat
    57541 cgtgatgcat caggccggaa acgagctggc ttaatttcgg gtaataacca accaccgcca
    57601 gccattcctg accggcgttt ttaccgcttt ccgctctctt tttctcgtgg agaataaact
    57661 gaaagctgtc actggtaacg acataacgtt cgccaatttc aatacgaata ctcatgccgt
    57721 tctccggtaa tgtttgtttt ttgcttcaaa gactgactgg caggaaacac aacgcgtggc
    57781 tgacggataa gccgcacgac gggcagcagg tattggcgcg tcacactctt cgcaaaccag
    57841 cgcagaaaca ccgcaatgct ttacccttgc cgcgttaatc tggcgctcca gtaattcagc
    57901 ctgttgttcc tgaataaaat ctacgttgtc cggcattacc agctcctttt gtcgttaagt
    57961 tttttaaatt catcagcgca atagctggca atttctgtcg ttaatttcgt cagttcgtcc
    58021 acggaggaga tttgcttgtg aaatacagcg cgtttaacaa gtaaattgac cacatcagac
    58081 aggagattta attcgttctg ataaatcgcg ataacagact cagttatttc gcgtttttct
    58141 ttatcaagac caagttgaat aagagataaa tcgccatttt tcataacggc gatttttaag
    58201 gcgttattca gtaatacaac tgaacgagaa caggacatca aagcacctcc ccgcgagaca
    58261 atccgatatt gtgaaatttt tccgactcct gactgagcag ctcgactatc tccacgcggg
    58321 ataactccgc ctttgtgata tggcgaatca tggcgtcaag atgagaagaa aagcgcgtcg
    58381 ctgcgtcggc ctgtgcttcg gttctggcct gttgcagcag taatgcgtat ttaccgcact
    58441 gattttcaga aactgtatgc atgactttct ccaggtaaaa agaagccccg cacaattaag
    58501 tgcgttaaaa actctggtta attacttaat gcagatattg ctctggtttt accgacgtca
    58561 gaattgtcgg tgcatactca aacagactga ataattcacg taatgcacgg aataaagcat
    58621 cacgccagta acatgactct tcattaattc gccagtatgg ctggttgaat tctttttcag
    58681 tcaatccggc atgcataaat aaagtacgac gctgactgac tgttaaaaaa ctaatatatg
    58741 catactcact tgcgccaacc tgacggcgtt ttgagaatgc accacgcaat tcatcaattg
    58801 cacataccag tcgttcacgt tcgacgtcgt tcatttcttc aaaacacatc gttgcgtgac
    58861 gctgttttaa ctgagcatgg aagcaaaccg ttaaccgttc gcgctccatc atctgattat
    58921 aataatcaca tgtatcctgc cagcgaggaa cggcaagatg cttaccaatt atccggcgca
    58981 tagctgctgg ctgtttttca acgagattga gcgtcatcac tgtcatttcc ataccctccg
    59041 gcttttcaga aaggtcagag ccttttttaa cggactctgt tttttggtgc ggataatgat
    59101 tcccttacgc cccttaccgt gggtgatggt gaagtcaatc gccctggggc tttcgttacg
    59161 cagtaactga gcaatacaac gaggctcatt catacggttc tccttaacgt ggttcaccga
    59221 gacctaacca catcaaccag ccgtcacgaa tctctttagg gcggctttca taagccagtt
    59281 ttagtccgtt attccatgcc ggaaggtata cccaatattc acctgcacga cctgaagctg
    59341 attgtggatc ggtcatatca attacaggca gctttccttt atcaatcatc cgacgaaccg
    59401 ctcctgtcga ttttcctatt agttttgcga actcctgata aggaatcgca tcagtcatga
    59461 gtgttacttg cttgctcatg tcgtcctcta gccctcatga attgcgttta atgtcttata
    59521 atgcctttta gtgcccacat ccaagcacta aacaatctac atctaaacta aatactattg
    59581 agatctaaac accatgtcaa acacgataag cgagaagata gtcttaatgc gaaaatcaga
    59641 gtatttgagc agacaacaac ttgctgattt aacaggggtt ccgtatggca cgctgagtta
    59701 ctatgaaagt ggtcgttcaa cacctccaac agatgtcatg atgaacatcc tgcagacccc
    59761 acaattcacc aaatacactt tatggttcat gaccaatcag atcgctcctg agtccgggca
    59821 aattgcgccc gctctcgcac actttgggca aaacgaaaca acgtcgcccc actccggtca
    59881 aaagactggt taacaattca tcgtgaatat attcatcaca agtgcctact attggtggct
    59941 aaatttcagc caccaagaaa aaagcgatta gtagtcgcaa aaaaacacac cactcggagg
    60001 gttttctgat ggcaatcaaa aaactcgatg atggtcgata tgaagtggac atccgcccta
    60061 ctggacgtaa tggaaaacgc atccgtagga agtttgataa gaaaagcgaa gctgtcgctt
    60121 tcgagaaata cacgttgtac aaccaccaca ataaagaatg gctatcaaaa ccaacagaca
    60181 agcgacgtct gtcggagctg acacagatct ggtgggattt aaagggtaaa cacgaagagc
    60241 atgggaaatc taatcttgga aaaattgaaa tcttcacaaa aataacgaat gacccatgcg
    60301 catttcaaat tacgaaatcg cttatcagcc agtactgcgc cacccgaaga agtcagggta
    60361 ttaaaccttc gagtatcaat cgtgatttaa catgtattag cggcatgttt acagccctga
    60421 ttgaagcgga gttattcttt ggtgagcacc ctatcagagg gacaaaaagg cttaaggagg
    60481 aaaaaccaga cacaggctat ctcacgcagg aagaaattgc cttactgctt gctgctcttg
    60541 acggcgacaa caaaaagatt gcgattcttt gcctgagtac tggagcacgt tggggagaag
    60601 cagctcgttt gaaagcagaa aatatcatcc ataaccgcgt cacgtttgtt aaaacgaaaa
    60661 caaacaaacc acgcaccgtc ccgatctcag aggctgttgc caaaatgatc gcggataaca
    60721 aacgaggttt tttattccct gatgctgatt accctcgctt cagacgaaca atgaaagcaa
    60781 taaaaccgga tttgccaatg gggcaagcca cacatgcact aaggcacagc tttgccactc
    60841 atttcatgat taatggagga agtattatca cgctacaacg gatactaggt cacacgcgga
    60901 ttgagcaaac tatggtttac gctcattttg cgccagagta ccttcaggac gccatttctc
    60961 ttaatccgct aagaggtggt actgaggccg agagtgtcca catagtgtcc acagtagagt
    61021 aacgtttaag ggctttcagt ggtaatttat gccgctcaaa cccgcattgt accgttgaaa
    61081 gcccctactg gtgacaccct aaatctccct tacacgggct tattttttat gcataagccc
    61141 tatccctggt caccgtcttc cattgaccac atcgatagaa tctcccttca tagcacgatg
    61201 cctttcacgt aacggcatcg tgctcgcaca ggttccggct aagcacaacc agaacgcgca
    61261 tgtttgacgc ttaccaaaaa atattctcac tctccacatt tgaatgtcag acgagcgacg
    61321 ccatgtaatc ctgcaccttc tgtcttcagg tcaactatct gcattttttt gccctgagta
    61381 acacagaaat gggctgcatc attttttact atattttctg caccagatat tctgcccctg
    61441 gctaaagaag cttcggcttc ggtgtagtat tggttatcga gtttacgctg aatattactt
    61501 ttatatgcaa gaccaaattt accgatactt gtctcatcat tatgcacagc acaaccagac
    61561 ataataaaaa tactaattaa tgatatagca gctatctttt tcatctcacc ttcccccatt
    61621 aaataccaac gacactctct agtgtttaaa tataataatg gcatgattat tataattgaa
    61681 taggattata ataaatgttc tgtacaacat ttcctacata agtaggaatt acggacattg
    61741 aggcccttca gggtaactcc atgggggctt taatatattt ggaatagtat atttaagatg
    61801 ccactgtttg gttgaatatt aggcatatgc tcttttttga aatttatcgg tggcagccgt
    61861 tagtattcgc tgtccccatt gcaagctcct ggtggtaacc actgaatcct ccatacttga
    61921 actgactttt tatcctccga ctttcatcct gttctgactc caccttttgt tttctgctct
    61981 acactatcta cagaccaatc ataaaggcac atacgatcat ggcagaattt cccgccagct
    62041 tactgattct taatggcaaa agtactgaca atctaccctt gcgcgaagca attatgctgt
    62101 tgcgtgagga aggaatgacg atccatgtgc gggtcacctg ggagaaaggc gatgccgcac
    62161 gatatgtaga ggaggcccgg aagttgggcg tcgcaacggt gattgccggt ggtggcgatg
    62221 gcaccattaa tgaagtttct acggcgttga ttcagtgtga gggggatgac atacccgcgc
    62281 tgggaatttt gccattagga accgccaatg attttgccac cagtgtaggg attcctgagg
    62341 cactggataa ggcgctgaaa ctggcaattg ccggtaacgc cattgcgata gatatggcgc
    62401 aggtcaacaa acaaacctgt tttattaata tggcgacagg cggatttggg acgcgtatta
    62461 ccacagaaac gccggaaaaa ttaaaagccg cgctgggtgg cgtctcttac atcattcatg
    62521 gcttaatgcg catggatact ctgcaaccgg accgttgtga aatccgcggt gaaaactttc
    62581 actggcaagg tgacgccctg gtcattggta ttggtaacgg gcgtcaggcc ggtggcggtc
    62641 agcaattgtg tccgaacgcg ttaattaacg atggcttgct gcaactgcgc atttttaccg
    62701 gcgatgaaat tcttccggct ctcgtatcaa ccttaaaatc tgacgaagat aacccgaata
    62761 ttatcgaagg cgcttcgtcg tggtttgata tacaagcccc acacgaaatc acttttaatc
    62821 ttgatggcga accgttgagc ggacaaaact ttcatattga aatacttccg gcggcgttgc
    62881 gttgtcgatt accaccagat tgtccattat tgcgttaatc aaactattct ctcttgaacc
    62941 agggagaatc cgaaatgccg gatgcggtgt aaatacctta tccggcatac agaacaatac
    63001 tacaccgtaa ttaattttac ccgtgtcttc tgcaatgcca gtttatccac ctctgataca
    63061 gcatcatcgg taataataat gtcaattttt tcgattggca gtacctgatt aaaaccgcga
    63121 cggttaaatt tcgatgaatc gagaacggca atcactttat tagcggcggt gaccatcgca
    63181 ccgctaatcg aataaccttc attaaaggtc gtaataccat taaccgcatc aataccatca
    63241 gcaccgacaa acattaaatc ggcattaata tcctgcaggg aacgttcagc aatagatcca
    63301 tgcatagatc gcgttttatg gcggaccgtg ccaccgcaga cgaccagagt aatgtcttta
    63361 ttttcggaaa gggcaaacgc agccgggaga ctgttggtaa tcaccgtgat atttttggcg
    63421 gtcattaatc cttcggcgat aagcattgtg gtacttccgc tgtcgagaat aacggtcatc
    63481 ccttcatgga tcattttgac tgccgcctgc gctattctgt ttttcggcgc gctggcgagc
    63541 tgaaatcgct ctttaaaccc gacttcctgg gtctcggttt cactattacc ggacattatt
    63601 ttcgccgcac cgccatgaaa gcgcgtaacc acgccttttt gttcgagaaa gcgcaaatcg
    63661 gcacggattg tcgcttccga ggcagcaaat actcccgcca gatcctgaac aagcacggtt
    63721 ccctgttcat tcactaattg gatgatctta ttccttcgct cgaatgaatt catcgtcatt
    63781 tacccatatt caattgtggc tagtgtaaac gaagggggac cattggtgaa tgatctgttt
    63841 tgcacaatac cgggccagca tcacgctggc ccgcggtttc agggaatgag caacactttg
    63901 cccggcatag cattacgagc gatgtcacgc accgcctggg cgaagctttc aaagcttcca
    63961 cggtgagcga ttaatggctc caggcttaac ttacgttctg tcagcaaccg gctcgccgtt
    64021 tcccactcct gccccggcca agggctggag tagttcatcc aactgccgat aaccgtcagc
    64081 tctttacgca atattttgcc aaacgttgcc gatgttaaat gcagatcctg atgcaacgtg
    64141 cccaccagcg ccagttgggc atgaggaccg gcaatctcta ccgccagttc gacagtttgc
    64201 ggtacgccag ccgtctcgag gataagctga ttaaagcgca gttcgcgtaa aacgctctgc
    64261 atttgcggcg cgctcatttc gctactgtta aatgtttgca tcgcaccgaa agattttgcc
    64321 agtgccagtt tttctgaact aatgtcgatc gccgtcacac tctttgctcc cagcgcgaca
    64381 gcgcactgaa tggccagcag gccaatggtt ccggcaccaa taataataac gtttttattc
    64441 tcacaacctt gcgctaaatg aaaagcatgc aggccaacgg taatcggttc aataaaagcc
    64501 ccatcctcaa taggcatatc agtaggtaga gcaaagacat ttttccgctt aacgacaata
    64561 tattcggcaa atccaccatc acgccgtgag ccaataaaat catattttgc gcactgggaa
    64621 taaaaccctt tcaaacactc tggacaagta aaacagggta ataacggcac acaggcaacc
    64681 gcatcgccag gatgtaaatc atcaacaccg gatcccaccg catcaatata gccgctaaat
    64741 tcatggccta acgttattgg ataataatgt gcaccatttt taaatatcct gggtaaatcg
    64801 gaaccacata agcccgagct ggcaattttt acccgcacct catcctgatg tttaatttca
    64861 ggaatgacgc tttctgcaac gcgcacgata ccatcagtat cattcaccac tgatttcata
    64921 aaaactcctg attgttaagg gggataacct ccccctcagg taaaaattat tctgcgagaa
    64981 cgactttctc ttgtttaata aagccacgcg ctctacgcca ggtcatgaaa ataccggtaa
    65041 gataaattgc gccgataata atgaatccgg gaatattttg cggggagaaa acctgaatca
    65101 gtaaccaggt aatgggagaa ccgccctgat ccattgaagc caccataccc ccggctttta
    65161 acgccccagc attagccgcc agttgggtgt gcaaaccaat agtttgcgtc gcgatccaca
    65221 gagtgatgct catgatgatg acacccgaga ttaaggtgcg gaacagattt ccacgatgca
    65281 cggcgactgc catcgccaca aagaagccga tggtggcaag atcgccaaac ggcagcacct
    65341 gattacccgg aacacaaaca gcaattaaaa tggtgagcgg gataaaaatc aggcttgccg
    65401 ataccaccgc cgtatgcccc agcagtaatg cgggatccag gccaatcagg aactcctgac
    65461 cgccgaactt cgcctgtaaa cgactacggg cctgcttagc gatgggcgtt aaaccatcca
    65521 tgatgggttt aatcacccgt ggcattagca gcatcactgc cgccgttttt accgccagct
    65581 gcaatacacc tttgacatcg taacccgcga ggatgccgat aatcagcccc atcacaaaac
    65641 ccacggtgac aggctcgcca aatggaccaa atttgcgctg aatatcgtcg gcgctaaatt
    65701 taattcggtt aacgcctggg attttctcga tgatagcatc gaccagcacc gcaatcggcc
    65761 ccatatacgc cgacgtaccg tgcggaatag caataccttc cagctcaaag aaatttcggg
    65821 tatcgcgggc aaaccagtcg ccgagcttat aaacaaacgc cgcgtgaatt accacacctg
    65881 ccatccctat catccatgaa ccggttgcca ggtgcagcaa cgcgccggtg aaggtcatat
    65941 gccagatatt ccagatatca acatttacca cccgcgtcat acgggtcagt aacatcgcca
    66001 cgttaaccaa tatcgcaatc ggaatcgcca ccagcgcaat ttgcgacgcc caggtcattg
    66061 gtgaagagcc cggccagccg acatcgacca catgcagatt caggtcgaaa ttttccgcca
    66121 tcgctttcgc cgccggacca atggaatcca gcattaagcc aatcacaagg ccaatgccaa
    66181 caaagccaat cccgatatgc agacccgctt taaagcaatc gcctgccttc atgcctaata
    66241 ttttagaaaa aataatgatg acaaccggca gcatcactgt agggccgagg tcgagaatat
    66301 aacgcatgac ttctgaaaac ataggtcacc cctgtaagat agtcagaatt ttattttgta
    66361 atgcttcgat accgacacca gaaacaaaag gcatgccgtg aactaacgga atatcgccaa
    66421 aactgcgatc ccccctggca gtggtacata tcaaatgcac accatccata taggtttcta
    66481 tttcattaac ccgacactgg attaattcaa caggaatatt atgactctga cacaactctt
    66541 taatttcttc cgccgccatc gtagaggtcg caaccgcgcc tccgcaagcg acaataatct
    66601 tgcgtttcat aaatagtcct ttttattgtt acagagggat ttattcgtct gaatctaaaa
    66661 catactttgt gaagtattcc tttaactggg tttcaggaag agtgattagt gtctcgacga
    66721 tatcgggctg ttgtaacttg ccaaataaac agcgtaaaag tttcaattgc tgctgcggat
    66781 tttccacaat caacgcaata accaacgata ccgccacgtc gttatcatca tccgcttgct
    66841 gaaaatgaac tttatttgtt ggccttaaca gataaatggc tgacgactta gcatgaatcg
    66901 cctcacaatg cggtattgca atggcgtgct gctcaagcat tatcccggta gggaattctg
    66961 cttctctggc aattaatgcc tgtggccagg tatcatgaac cacacctttg gcgagcatct
    67021 cattaccgat atgggttaaa acttcgctac gatcgacaaa agaaattccg ctacgaacaa
    67081 acaggttagt catataccgt ccttattccg cacagccgta gcgataggcg cgcaaaacat
    67141 cataaatttt atccataatc agctgatgcg gtattgctga taattccccg gactgaatgc
    67201 gttcaaattg tttgggaaga tactgactaa tcatgcccag tgggatgtcc acgccttcaa
    67261 gattcaccat catcgtttcg acgctatttt taatccgact atgcggccag taataacgaa
    67321 tacgatccga caggctgtaa cgaatatcca gtaatgaatc gttaaaaccc gtacgataat
    67381 attttttcca gtattgcggt tcgtcgagca tcacttcttc aattaccgcc aggcaaccgc
    67441 tgcgattttc aggggcgata agttcctgct caatttgtgc cagtgcaaat atcgcctcgc
    67501 gtaaagcaaa ggttaatgcg ggaccgactt tcaatattgc aaagtgatcg cggactaatt
    67561 cccaataagc cgtccgggtc tggtaatcgg tagaatgtgc ttcataaacc attcgggtgt
    67621 tttctatcca ttgcgccagc ggctgcgctt cctgcggctg ataatggata atattgctgt
    67681 gatcaaattc cacgcccggc tgcaccacga tggcaatcac gcgtgttaac gcctctgtca
    67741 gcccacgggc aataaaggcc ttttgatgcg tacgtaaagt attggcggta tcttcaacat
    67801 gggtgatgtg tactgactga atggcgctgg cctcaccgcc cggaaccgga acttcggtgc
    67861 caatgacata gctcagttgc tcacgctggc aatctgtcgc cacactttcc gcagcaaagc
    67921 aaagcacagc agctcgttcc gcaaccgttt ctggtgctaa cggtatggga tcccccgcgc
    67981 aggacattga cgcatcaaga tgaattttac tgaagccggc acgaacatat tcctttacca
    68041 gctcgacgga tttttccatc gccgcatccg cattttcttg ctgccagcag tttggcccca
    68101 gatgatcgcc gccgagaata atgcgttcgc gtgcaaaccc aactttatcg gcaatcgtaa
    68161 aaacaaattc gcgaaagtct gccggtgtca ttccggtata accgccaaat tgattgacct
    68221 ggtttgacgt tgcttcaatc agcactttgc gcgtgctgtt gcgatcaaat gccagcgccg
    68281 cttcgataac caacggatgg gcagaacaga ctgaacatat gccgatatgt tcaccagctt
    68341 tatgccgggc aattaacgtt ttcatgtttt ttccttatta aatggcaggt gcgttatgcc
    68401 ctgccctagc agccacaatc ggcaatcact ttgctcacca catcgcgcat tgcggattta
    68461 gccgactgca aataatcccg gggatcggtc gcttcagggt gctcggtcag gtaatttttt
    68521 aacgcctgcg agaaggcatt tttcagctcc gttgcaacgt tgattttgca tatccccagt
    68581 ttgatggttt gctgaatatc cttagtcgat aaccctgacg cgccatgcag caccagcggt
    68641 aagttcaccc actggcgaat gttctccagt cgagaaaaat caagcgccgg tgcgctggca
    68701 tacatcccat gagccgtgcc gatggcgacc gccagggaat caattccggt tgcctcggca
    68761 aattcacgcg cctgagcggg gttggtataa aaagcatcgg cttcattgac ttgcacatca
    68821 tcttcctggc cgccaagttg ccccagctcc gcttcgacgc tgacatcaaa gcgatggcaa
    68881 aaatccacca cctctttgac ccgtgaaata ttttgcgcaa aaggcaaatg cgaggcgtca
    68941 atcatgactg agcgcacgcc agaacgaacc ttctgagcga tatcgtcaaa tttcgtgtga
    69001 tggtcgagat gaattgccag tggatggtga tattgcttcg ccatcgcgct gaccagcgcc
    69061 aacagatttt ctgtaccagc atgagtaaat gtgccaggcg ttccggcgat gatgaccggc
    69121 gcatgcaggt tggcagcggt ttccaccacc acttgcatcg tttcgagatt gtgaatattg
    69181 aatgccggaa ccgcataacc gccgcgctgt gcgttgttca gcatctgctt tgtcgatacc
    69241 acgtacattt tcatatcctg tcgtttgttt tcgatttcaa aatataatga aatgatttgt
    69301 ttttaaatgt cgagataacg atcacaaaaa cgacaatgtg aaaattgttc gaggagtaaa
    69361 aggcaaaaaa aaacggcctc ccgataggga agccgtagca aagtgcgcgt gtttttatgc
    69421 cggatgcggt gtaaacatac gtggcagtgc tatgtaggca tgataagacg cttccgcgtc
    69481 gcatcaggca taggttgccg gatgcagtgt aaacgcctta tccgtcctac ggggtggtgc
    69541 tgttcgtatg aggcgtatca ggcaatagta attttgctat cgagataaac gtcctgcacg
    69601 gcattaatca gtttcacgcc gtcagccatc gatttcttga acgccttacg tccaagaatc
    69661 agccccattc cgccagcgcg tttgttgata accgcagtac gcactgcatc actgaggtca
    69721 gtttcaccgc ctgcagcacc gccggagtta atcagccctg cgcgccccat atagcagtta
    69781 gctaactgat aacgcaccag atcaatcggg ttttcgctgg ttaacttgct gtacacgcga
    69841 tcgtcggtat aaccgtaatt aattgcttta tagccgccgt tattttccgc cattttttgt
    69901 ttgacgatat ctgcaccgat ggttgccgcc agatggtttg cctgaccggt caggtcggcg
    69961 gaaacatggt aatcaacgcc atctttctta aaggcggagt tacgcaaata ggcccacagc
    70021 actgtcacca tacccagctc gtgcgcacgt tcaaaagccg cagaaatttc ttcaatctgg
    70081 cgacgtgact cttccgagcc aaaatagata gtcgcaccaa ccgcaaccgc gcccatgttg
    70141 aacgcctgct ccacgctggc atacagcgtt tgatcgtagg tattcgggta acttagcgtc
    70201 tcgttgtgat taagtttgac gaggaatgga atgcgatgcg cataacgccg cgataccgac
    70261 gccagcacgc cgtaagttga cgccacacag ttacagcccg cttcgatcgc cagttcaaca
    70321 atgtttttcg ggtcaaagta gagcgggtta gcagcaaatg aagctccggc agagtgctca
    70381 acgccctggt caaccggcag aatagaaaga tatcctgtgc cagccagacg cccggtgttg
    70441 tacaacgtct gcatattacg taacaccgct ggcgggcgat tattgtcaat cattacgcgg
    70501 tctacgtagt catgtccggg gagataaagc tggtcagaag gaattgtcat acaacggtgc
    70561 tgtaaaaggt tgtcggcgtc tttgccaagc aactgcgcaa tatctgtcat tactatgctc
    70621 ccgtaaattc cgattggata tcggctatgg attgtcctgg cccgcctttt gcgggcaatc
    70681 ataatcctgg tcgttacggg cgagtttttc catcttttgg atgttttttc agcgttttct
    70741 gttggctcga ttcatcagaa aaaatgttag cgcggtcaag ttttcaccgc aaaggtattt
    70801 aaaaggtatt attaagtggt attatcatcg cgtaccttac attacctgtc atgaaggaat
    70861 taaaagatga aaacaacagc aaagctgtcg ttcatgatgt ttgttgaatg gtttatctgg
    70921 ggcgcgtggt ttgtgccatt gtggttgtgg ttaagtaaaa gcggttttag tgccggagaa
    70981 attggctggt cgtatgcctg taccgccatt gcggcgatcc tgtcgccaat tctggttggc
    71041 tccatcactg accgcttttt ctcggcgcaa aaagtgctgg cggtattgat gttcgcaggc
    71101 gcgctgctga tgtatttcgc tgcgcaacag accacttttg ccgggttctt tccgttactg
    71161 ctggcttact ctctaaccta tatgccgacc attgcgctga ctaacagcat cgcttttgcc
    71221 aacgtgccgg atgtggagcg tgatttcccg cgcattcgtg tgatgggcac tatcggctgg
    71281 attgcctccg gtctggcatg tggtttcttg ccgcaaatac tggggtatgc cgatatctca
    71341 ccgactaaca tcccgctgct gattaccgcc ggaagttctg ctctgctcgg tgtgtttgcg
    71401 tttttcctgc ccgacacgcc accaaaaagc accggcaaaa tggatattaa agtcatgctc
    71461 ggcctggatg cgctgatcct gctgcgcgat aaaaacttcc tcgtcttttt cttctgttca
    71521 ttcctgtttg cgatgccact ggcgttctat tacatctttg ccaacggtta tctgaccgaa
    71581 gtcggcatga aaaatgccac cggctggatg acgctcggcc agttctctga aatcttcttt
    71641 atgctggcat tgccgttttt cactaaacgc tttggtatca aaaaggtatt attgcttggt
    71701 ctggtcaccg ctgcgatccg ctatggcttc tttatttacg gtagtgcgga tgaatatttc
    71761 acctacgcgt tactgttcct cggtattttg cttcacggcg taagttacga tttttactac
    71821 gttaccgctt acatctatgt cgataaaaaa gcccccgtgc atatgcgtac cgccgcgcag
    71881 gggctgatca cgctctgctg ccagggcttc ggcagtttgc tcggctatcg tcttggcggt
    71941 gtgatgatgg aaaagatgtt cgcttatccg gaaccggtaa acgggctgac tttcaactgg
    72001 tccgggatgt ggactttcgg cgcggtgatg attgccatta tcgccgtgct gttcatgatt
    72061 tttttccgcg aatccgacaa cgaaattacg gctatcaagg tcgatgatcg cgatattgcg
    72121 ttgacacaag gggaagttaa atgaaaacag aacgtattct cggtgctctt tatgggcagg
    72181 cgttagggga tgcgatgggg atgccctccg agctttggcc acgcagccgc gttaaagcac
    72241 actttggctg gattgaccgt tttctgcctg gaccaaagga gaataacgcg gcctgttatt
    72301 ttaaccgcgc cgaattcacc gacgatacct cgatggcgct gtgtctggcg gatgcgttac
    72361 tggaacgtga aggcaagatc gatccggatc tgattgggcg taatattctc gactgggcgc
    72421 tgcgtttcga cgcctttaac aaaaacgtac taggtccgac ctcgaagatt gcgcttaacg
    72481 ccattcgcga cggtaaaccc gttgctgaac tggaaaacaa cggcgtgacc aacggcgcag
    72541 cgatgcgcgt ctcgccatta ggttgtttgc ttccggcgcg tgatgttgat tcctttattg
    72601 atgatgtggc gctggcgtcg agcccgaccc ataaatccga tctggcagtt gcaggtgcgg
    72661 tagtcatcgc atgggcgatt tctcgtgcca ttgacggaga aagctggtca gcgattgttg
    72721 attccctgcc ttcaattgcg cgacatgcac agcaaaaacg catcactacc ttcagcgcct
    72781 cactggcagc acgtctggag attgcgctga aaattgtgcg caatgccgac ggcaccgaat
    72841 ccgccagcga acagctttac caggtcgttg gcgcaggtac cagcactatt gagtccgttc
    72901 cgtgcgccat tgcgctggtt gaactggcac aaaccgaccc gaatcgctgc gccgtcctgt
    72961 gcgctaacct tggcggcgac acagacacca tcggcgctat ggcgacggcg atttgcggcg
    73021 cgttgcatgg cgttaacgct atcgatcctg cattaaaggc ggaactggat gcggtaaatc
    73081 agcttgattt caaccgctat gccacagcgc tggcgaaata tcgtcaacaa cgggaggcgg
    73141 tatgagcggc gctcgattac acacgctgct gcctgaatta acctcgcgtc agccggtgat
    73201 ggtcgtcggc gcggcggtca ttgatgtgat cgccgacgct tatgccctcc cctggcgtgg
    73261 gtgcgatatc gaactgaagc agcagagcgt taacgttggc ggctgcgcac tgaatattgc
    73321 cgtggcgtta aagcgcctcg gcatcgaagc gggtaatgcc ttgccgctcg gtcagggcgt
    73381 gtgggcggag attattcgca accggatggc gaaagagggg ttaatcagcc tgatcgataa
    73441 cgccgaaggt gataacggct ggtgtctggc gctggttgag ccggatggcg aacgcacttt
    73501 tatgtcattc agcggtgtgg aaaatcagtg gaatcgccag tggctggcgc gattaaccgt
    73561 tgcgcctggc agcctgctct atttttccgg ttatcaactg gcctcgccct gcggcgaatt
    73621 gttagtggaa tggctggaag agctgcaaga cgtgacgccg tttatcgatt ttggcccacg
    73681 tattggcgat atcccggatg cattactggc gcggatcatg gcctgtcgac ctttagtgtc
    73741 gctcaatcgt caagaggctg agattgccgc cgaacgtttt gctttatccg cagagataac
    73801 aacacttggc gagcaatggc aggagaaatt tgccgcgccg ttgatcgttc gtctcgataa
    73861 agaaggcgca tggtatttca gcaacgacgc ttctggctgc attccggcat ttccaacgca
    73921 agttgtagac accattgggg cgggcgacag tcatgcaggt ggcgtacttg ccgggctggc
    73981 ctctggtctg ccactggcgg atgccgtatt actgggcaat gcagtggcgt cgtgggttgt
    74041 cgggcatcgg ggcggtgatt gtgcgccaac gcgcgaggaa ctactcctcg cacacaaaaa
    74101 cgtatagatc gctgcgacag tggctaatgc tgtactcaat aggccgttgc tgttggtcaa
    74161 gcgccacttg cttgatcacc agcaccggta ttttgctatc taactgaata tgtgactgaa
    74221 actcggcatc cggcatccgg gcgctaacgc gggaacgcgt acgttgtagg taaatgtgct
    74281 ggctgcggaa atagtcataa agtgaaatcc cgatggcatc aacatcatga attaaatgcg
    74341 ccggaaccca cgattcctca atcgataccg cttcttcatc gacatagcga atacgcttaa
    74401 gcaaaaagac atcacttcct gcttcgaccg ccagctgctg cgcgacttct tcgggacatt
    74461 tcacgacacg cttgttaacc catagcgtat cgggcttttt cccgcgcaat accacctgct
    74521 gagaaaaacc tcgcgcttct ttcaacgagt attcgaagat gttgttgatc tgcgtgccgt
    74581 aaccgcgcga acgcgtcacc acaccctctt cttccagcgc ctgcatcgcc ttgcgcaccg
    74641 taatgcgcga aacgccggtt aactgactta aatcacgctc accgggcaaa atattgccat
    74701 gctccagcac cccgctgcgc acggcatttt ttaccgtttc ggcaaactta atataaagcg
    74761 gcgtgttatc cgccgctaaa atacgctcgt tcaactgagc gataagctgg gtatgcgctt
    74821 gttccatttg tctttttcct gccggggagt ctggtgggag tatatcactg cttcttattt
    74881 aaggcgtttc tttaatccca tcaacgaatc cctgcaactc ctccaccgtg ccattaaaca
    74941 cattaaaatc caccggacca ttgatgccat ctacctgtcc acggtcggaa tactgccaga
    75001 agcgccaggc cattccgtca ttgtccggac gacgttgata atagtgagcc acccaccacg
    75061 gatattcatt aaaatagccc gccagattgg tgtgataaaa aacggttcct gagtaaataa
    75121 tcggcttttt tcccgtactt ttttcgacca tttttagcca ctgacttact cgtttgcgta
    75181 attcttttgc cgataatttt ccccgttctt ctacgtccag tacagcaggg aaatcgcctt
    75241 gtgagaaatc caccgtttgc agaaataatc gtgcctgaac cggcgctgct accgacgggg
    75301 agaaataatg atacgccccg cgcagcaggc cattttcgcg gcttagttgc cagttacgcg
    75361 aaaaatacgg gtccaccagc ttttcgcctt ccgtcgcctt aataaaagca aactgtaagc
    75421 ggataccgtt gtcgcgcatt tttgccaccc gctgccagtc gatccgctcc tgccagcgag
    75481 aaacatctat tccgtgaatg gtgtaactgg caggaatgcg aatagcgaaa gatttaaccg
    75541 ggcgataacc gtagaaatgg atgtagtctg taatctgaac tgctgtcgaa tagaaaaaat
    75601 tcaccgtctg acgcggataa attgcaacga tggaaattag cccaagagcg cataatagag
    75661 aggtgagttt ttttcgactg gtaattctta gctgcattgg tttcatccct gaatgtcagt
    75721 gccaaaggct gacaataacc aaagcagcta tagtacggtg ctcaccacca ggcgtggaag
    75781 tggtgaaccg gaccaatacc gtgaccaact tccagcgtgt cggcctgggc taatgctgat
    75841 gaaagccagc tttttgcctc ctgtacggtg tcagcccagt ttgtatggcg cgggcgtagt
    75901 gcagccaacg ccgcagagag cgtacaacca gtgccgtggg tgtttttggt cataatgcgc
    75961 ggtgcggtaa accgttgttc accctcgcgg gtaaacagcc agtccgggct ttgctcatca
    76021 tccagatgac cacctttcat tagcactgcg ccacagccca tcgccaacag cgatcgccct
    76081 tgttccagca tttcctgttc ggtgcgcgcg tgtggcgcgt cgagcaaggc ggcagcttcg
    76141 ggcaagtttg gcgttattaa tgaaacctgt ggcaataatc gactgcgcag cgtagcaacc
    76201 gccgaaggtg aaagcagcgg gtcgccgctt tttgccagca taacggtgtc gagtaccacg
    76261 ttttggatct gataacgttg caaccgttct gccaccgctt caacaatatc ggtttccgcc
    76321 aacataccga ttttagtggt gtcgattcgc acatcgctga acaccgaatc gagctgggcg
    76381 gcgacaaaat caggctcaat gcgatacacc gactgtacgc cacgggtatt ttgcgccacc
    76441 agtgcagtaa taactgagca accataagcg ccaagtgccg agaaggtttt aagatcggcc
    76501 tgaatccccg caccaccact cggatcagta ccggcaatcg tcagagcgtt aattcgtttc
    76561 atgcctgcac ctcctgcgtc aattgccaga gcgcatcaag gaaatgtgga acaaaactgc
    76621 ctggcccctc gcttctggcg actgcgcgtt ctccggcttg tttcatccag tgacaggcag
    76681 atgcgacatt ttccagcgta tcgcctggta acgcacagca ggcagcgaca accgccgata
    76741 atgcacagcc agttcctacc actttggtca ttaacggatc gccaccgtga atgccaacgg
    76801 cgcgatggcc atcggtaaca taatccacct cgccagtgac cacgacgatt gcgccagttt
    76861 cccgtgccag tgtttgtgca gcgggtatcg cgttagctgc ggcgtcagtg gtatccactc
    76921 cccgtccacc attagcaatg ccagctaatg ccatgatttc cgaagcatta ccacgtatcg
    76981 ctgccggttt aaaagataaa agttcatgac aaaaatggcg gcgataatcg agcgcaccaa
    77041 ccgctactgg atcaagcgtc cagggtgttt gagagctttt tgcttgctca acggcagcac
    77101 gcatcgcctg agcgcgtggc tgcgtcagtg tgccaacgtt aatcaacaag gcactggcga
    77161 tagccgcaaa ctgactggcc tcttcggttt cgataaccat cgctggcgat gcaccgagcg
    77221 ccagcaaggt attggcagta aaggtttgca ccacatcgtt ggtcatgcag tgcacaagag
    77281 gggaatgttg gtgaaaaagg tgtaacgcgt gcgcagattg cgctgaactc agcaggtcga
    77341 cttgcatagt ttgctcctgc cataacgtga agaagcaatg acctggtggt ccgtgacttc
    77401 cctacgctgg cattatccag atcaggtgat acgggtattt ctcagccttc acgcagaagg
    77461 gcaccccgag tcgtttggtt gcgatgataa atcgcagagg aggatggtaa tgtccagcgc
    77521 acgcgttgta aacgaggaaa aacggcagtt ttacaatcgc gttaataaat tatttgatat
    77581 atgaatccag caccttcaga acgacatcca gatcttcttc acgttttagc tcatccccct
    77641 ggtgaacgat gtgttccgtc agatgacctt taatcacttc ccgcatcaga ccgtttaccg
    77701 cgccacggat agcagcaatc tgttgtaaaa ctgcagcgca ttcgtgcggc tcgtcgagca
    77761 ttttcttgag cgccacgacc tggccctgaa tcttactggc acgcgctttc agtttctgtt
    77821 tatcacggat tgtatgagac atggcaacac ctggttaaca agaatatgaa aaatcatagc
    77881 actattaatc tactgggggg tagtatcagg tactgggggg gagtagaatc agattgccga
    77941 attaatacta agaattatta tcatgaccga atttacaact cttcttcagc aaggaaacgc
    78001 ctggttcttc atccccagcg ccatcttact tggtgcgctt catggcctgg aaccagggca
    78061 ctcaaaaacg atgatggcgg cgtttatcat cgccatcaaa ggcaccatta aacaagcggt
    78121 gatgctcgga ctggcagcaa ctatttcgca taccgcagtg gtctggttaa ttgcctttgg
    78181 cgggatggtg atcagcaagc gctttactgc tcaatcagca gaaccgtggc tccagctgat
    78241 ttccgcagtg atcattattg gcaccgcgtt ctggatgttc tggcgtacct ggcgcggcga
    78301 acgcaactgg ctggagaata tgcacgagta tgatcatgaa catcatcacc acgatcacga
    78361 agatcaccac gaccatggac atcatcacca tcacgaacat ggcgagtatc aggatgccca
    78421 tgcacgagcc catgccaatg acattaaacg acgctttgat ggtagagagg tcaccaactg
    78481 gcaaattttg ttatttggct taaccggtgg tcttatcccc tgtccggcag caattaccgt
    78541 gctgttgata tgcattcagt tgaaagccct gacactgggc gcaacactgg tcgtcagttt
    78601 cagcattggc ctggcgttaa cgcttatcac cgtaggcgtt ggcgcagcaa tcagcgttca
    78661 gcaggtcgca aaacgctgga gcggatttaa cactctcgct aaacgcgccc cctatttttc
    78721 cagtctgttg attggcttag tcggtgtgta tatgggcgta catggcttca tgggcataat
    78781 gcgataactc aatcctttag gcttgcatgt tatcttcagc ccaggatgat tcttaaatca
    78841 gctatttccg ctgactctct gctcgctaag gatgctttta gggcatcctt tcatttacac
    78901 tttttacgaa atcatgggat cactaacaaa atatcgcttg tcagttatat tgtatggcag
    78961 gaaagatatg cgactgatat tacagatccc caaagtggag agtttatgac cattaaaaat
    79021 aagatgttgc tgggtgcgct tttgctggtt accagtgccg cctgggccgc accagccacc
    79081 gcgggttcga ccaatacctc gggaatttct aagtatgagt taagcagttt cattgctgac
    79141 tttaagcatt tcaaaccagg cgataccgtc ccagagatgt accgcaccga tgagtacaac
    79201 attaagcagt ggcagttacg taacctgccc gcgcctgatg ccgggacgca ctggacctat
    79261 atgggtggcg cgtacgtgtt gatcagcgac accgacggta aaatcattaa agcctacgac
    79321 ggtgagattt tttatcatcg ctaaaaaaag ccccctcatc atgaggggga aatgcagaca
    79381 ccttgttatt ttttattatt agccacttgc tcgtcttgct tgttattagt cgtatttcac
    79441 gttgattaat gcggttgcct ccagtgcgcc agatttaact ttgtttgtat cgtagacgta
    79501 gtaactggca gttatcggaa ttgaaaccgt tgtatctgta agttttgact ttgccgcaat
    79561 ttcttttaaa tttgtgtatc ctttactgct gttagtgata tttaaaagtg tgactgacga
    79621 accattactt tcaagaataa atcctacgcc attatcttcc gcacctttca gtattgtgtt
    79681 gctgttggtt aacaagtcag aacttgataa atacacctgc attttccgtg atgtttgatt
    79741 ttgttgacct aataaatttt tacaacgcag aataatatcc ccttgtttac tatttattgt
    79801 tgctttgtta cctgtggctg gtaattgtga aacgggaata ctgtctaaag taattaataa
    79861 atcgtccggt ttgcaggttg tggttatcgg gttgtatgac aatgtcagat taaaggaata
    79921 gacacctgca ttttgtgcca atgcatttct gtagctattt acacatgcat cccatgactt
    79981 gccacctaat aaacacagta gaaattgtac tgcggcactc ccgcgtgtcg atgatgaagc
    80041 actggataat gtcgcaactc cattaatcat cactgaaccg ccgccccccg cagaaacatt
    80101 cacttttgca ttactggccg caggtgtcac cgttaccgaa taaccactgg tgaaactggt
    80161 ctgattattt gataacgtga tgttgttttt attggtccat tcaaaatgta aatttaattt
    80221 aatggtatcg ttataaagtc caacaataat atccgacggg ctcatagggg tgatagtatt
    80281 tgtactcacg catgacgaat cattaaaatt atgctcatat gtcgccattt gtgtttgagt
    80341 aagtgcaaaa tccccctgaa caaccaaatt gtgctgaaca gataactcac tataacatgc
    80401 cgcatagctc acaggcataa ccatcatcat tactataaat aacattatac gaatattcat
    80461 aaccacctga ctattactgg caaatatagt ttctactttc atcaatctct ttaccgaagg
    80521 tgattgtgca tgaaagtcct tgttgcttat caattgctac attaaccgat ggcggtactt
    80581 cattggtgcg aataaataac tggcttccct gaccgacaac gccaatatta tgaccatgga
    80641 tatcattgac ttcataacca aacattaatg gttgcccatc cgctcttaac gcttttataa
    80701 accagggctt gcgctgatcg gtatcaaaat taaccagtac aaccgcgccg cgataagggg
    80761 cggcaatttt ccggttgcca cgtaattctg cttcgctatc gctttgcgac acatccagca
    80821 tcaggtgatt ttcccgataa ggtgtcattc cgtcgtatac caccactcca ttacggtttg
    80881 ttgtgcgata tttttgccca ttgacataag catctttaat tcctggcgca ttcatcacag
    80941 caaacgtttc ggaaagacgg ttcgccagat taacgccccc cgaccaggcg acaatgcccc
    81001 ctgaaacact ggctccagcc tgtcgataag tactcgactg actataactg ccattcactg
    81061 tcgcaaccgg cgcgttccag gtcaaattcg ccccagctgt cgtttcattt ccctgattct
    81121 gataactcag gttgacccca tagttaaact gatcccggct tccaacggtt cctgataatc
    81181 ccgtattatt tgaggcaaac ccctgatcat caaacgtcgt tgagttagac atatatattt
    81241 gccgacgagg cgtcgtaacg tcatcacccc aatcaaaggg aatcgatata aaaatattaa
    81301 aacgtttctc ttcatgatga ttctcgtcat acgcctggct tgccgcgagg atatagctta
    81361 tccgccgcca gttattggaa taactcaact gataatcctt actgctgccg ctacgccccc
    81421 agtaatctcg ccataacgta cttaaggaca cagaccccca gccttctggc aatgactggc
    81481 tcatattggc agagaagcta tttttacggc caaaatcgtt ctggtaataa tcggcaatgt
    81541 catagacatc gttttcatca cggcgataat tatctttatt gtttgcccaa acgtgatcat
    81601 taaatgtccg gtaatcacgc gatgaataac gccaggccgc cagaccaaaa cgcgtcgacg
    81661 tttggctcac aaatttgttg taggcaattt gataactttg cccgtcaaac acatcgccgt
    81721 tgtcttgttt actatgcgac ttcgtggcat cgacggaaat ggcaccaaag cgtgtattcc
    81781 agccagtccc caaagtaaac gcgtaataat tattcgcgac catcgagcca ccatacagcg
    81841 tcaataaatt attaaaacca tactgataac ccgcctggac aaaatcactt tgtttgctcg
    81901 ccccttcaat atggctacga cccgccgcaa aatcatattt cgatacgccg ggttgcagca
    81961 tatttggcac tgctgcataa ggcaccagat aggtggttac cgagccgtcc gcctctttca
    82021 cgctgacatc aagatctgct ccaccaccgg ccaactgcaa atctgtaatc gcgaacgggc
    82081 caggaggaac ctctttctga taaaccacaa aaccgttctg ttcaatagtt accagcgcat
    82141 tactctgagc aatcccctgc acccgtggcg taaaattttg tttcgagtta ggcaacatct
    82201 gcatatcacg aaacaaccgc acaccgctga agcgaacaga atcaaaaata tcgcttgatg
    82261 tgtacatatc acccacgcga agcgtgccga gaagttgggc aaatccacgt tccagataca
    82321 gggtattgct tttccacacc cctggattgt tatttgtttt actgaaactg gcatcagaat
    82381 gcagttgcca ccccagtaaa tttaacccgc tgttaaaacg tacatatgta ctcttactat
    82441 tacccgacgc tttatagtca ctgtaatact gactcacata ataagaggta taaaacgcat
    82501 taataccccg ctcccagttt tccggcggaa catagccact ttccagttct tccacccagg
    82561 cctgcgggac actgaaatcg agacgaaaaa ccccgatatc ccaggtatag ctcctaccct
    82621 gaacaagttg ctcaaatgtt aaacattgct taccgctggc gaagttatcg ctattaatgc
    82681 ctaaccgctt gataacttct attgataaac atgtttcttg cgggttgtct ttaacaataa
    82741 tctcatattt cccgcgccat tgcttattga cataaatatc gatgtcatac tgccccggta
    82801 agggttgatt atcatcaaga cgaatatttg caacctgctg gtctttcatt ccacctatca
    82861 taaaatgggt atcaaaggtt tcttcagctg cataagcttc aatgccgagc aataacgcta
    82921 cgattgctga tgcaagtggg gtcattctca acataggcag ctcctgcaat taaattttgt
    82981 cactaatata gttgccgtgg tcatcgataa tggtcagata ccagttattt gcattattac
    83041 ttttgacatt aacactctga ctttctaagg gggcaatcat aatagtttca taattgactt
    83101 taacattatt agctttgaca tccgaaatcg tcacccaatt agctgagtca ttttttatca
    83161 ccaatccatt gccactgcga tttaccagta actttttaaa ggtcgcttta tttaccggag
    83221 caatacccgc tggccggtaa aacaacttaa ttctgttttg catcgcaaac ttcagtgcat
    83281 ttttgccttc ttgctctgga ctatttggtg gaatatccag aacattcaga taaaaaatgc
    83341 tttctttatt agtgggcagt ttattcggca taattttgat ttttacttgc tgcccggaat
    83401 ttgcccctat ttttgccact ggtggcgtta acatgaaagg aacctgaatt ttttctggtg
    83461 gtaacgacgt atcgccatca tcaatccacg cctgcagcag cgaagaacgg tttccctggt
    83521 tcatcaactg caccataact tctttatttt ctgccgggta aataatgcgc gtcccgtaga
    83581 taacaattcc ggcatgtgca ggaaggacca tagaaaaaat gagtaaagat aataatcctt
    83641 tcatcttaat gcctcttaaa ttaaaaagcc gccatgggga tagcggccat gtttattacc
    83701 tggagaaact tacaatttta tttattaatc ctgcgccaca cgcataacca gagaagataa
    83761 cacatcacct gattgcactg agttatctgt cagtttcagg tattttgctt taaacggaat
    83821 agcatattga ccttccgctg ttttataact ctcaatgtta aaaggtgcag aaacatccag
    83881 gacatcgctg ttatcaccta aatagagata gtaacccacg gtatcgttgc tactggctat
    83941 cgccttatcg ttaccggtta ttttatcgtt ggtaatcaac gagagtttta caccagcaac
    84001 ttctttcggg cagctaattt ttaacatgct gctggttaca ccgactgcat catcataggt
    84061 agaatattta gctttacctt caaagacagt ggttggtaca gtcggcatat taatacttac
    84121 tgaatcacca cccgcttcga atttacacgg agattctgca atattcccct taatagttaa
    84181 tgttccggta tcaacgtctg cagcaaatac tccagcgctc ataaaaaaag aagaaaagac
    84241 agcggcagca ataattgaac gtttcataat atgttcctta ataaaattta tttttggttg
    84301 ttaagagtct gtatctcttg gaaacaaatt tctcacatca aaaaagttgc cgcaatgtaa
    84361 acacattgtt ttattgattt aaaacaaatt aaccattgca atataaatac aaaaaatgtt
    84421 taagagtatt aac
//
LOCUS       DAZFXT010000004        84240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.004, whole genome shotgun sequence.
ACCESSION   DAZFXT010000004 DAZFXT010000000
VERSION     DAZFXT010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 84240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 84240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..84240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.004"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            49..567
                     /gene="yfgH"
                     /locus_tag="ACHKW0_000280"
     CDS             49..567
                     /gene="yfgH"
                     /locus_tag="ACHKW0_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7473175.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            583..1122
                     /gene="yfgI"
                     /locus_tag="ACHKW0_000281"
     CDS             583..1122
                     /gene="yfgI"
                     /locus_tag="ACHKW0_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HFO7473176.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQKQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1217..2794)
                     /gene="guaA"
                     /locus_tag="ACHKW0_000282"
     CDS             complement(1217..2794)
                     /gene="guaA"
                     /locus_tag="ACHKW0_000282"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HFO7473177.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2863..4329)
                     /gene="guaB"
                     /locus_tag="ACHKW0_000283"
     CDS             complement(2863..4329)
                     /gene="guaB"
                     /locus_tag="ACHKW0_000283"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HFO7473178.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4491..5861
                     /gene="xseA"
                     /locus_tag="ACHKW0_000284"
     CDS             4491..5861
                     /gene="xseA"
                     /locus_tag="ACHKW0_000284"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HFO7473179.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDLQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQTVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5858..6073)
                     /gene="yfgJ"
                     /locus_tag="ACHKW0_000285"
     CDS             complement(5858..6073)
                     /gene="yfgJ"
                     /locus_tag="ACHKW0_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HFO7473180.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6142..7614)
                     /gene="der"
                     /locus_tag="ACHKW0_000286"
     CDS             complement(6142..7614)
                     /gene="der"
                     /locus_tag="ACHKW0_000286"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HFO7473181.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="ACHKW0_000287"
     CDS             complement(7732..8910)
                     /gene="bamB"
                     /locus_tag="ACHKW0_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HFO7473182.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="ACHKW0_000288"
     CDS             complement(8921..9541)
                     /gene="yfgM"
                     /locus_tag="ACHKW0_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HFO7473183.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="ACHKW0_000289"
     CDS             complement(9559..10833)
                     /gene="hisS"
                     /locus_tag="ACHKW0_000289"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HFO7473184.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="ACHKW0_000290"
                     /gene_synonym="gcpE"
     CDS             complement(10944..12062)
                     /gene="ispG"
                     /locus_tag="ACHKW0_000290"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HFO7473185.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQQVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="ACHKW0_000291"
     CDS             complement(12089..13102)
                     /gene="rodZ"
                     /locus_tag="ACHKW0_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HFO7473186.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13387..14541)
                     /gene="rlmN"
                     /locus_tag="ACHKW0_000292"
     CDS             complement(13387..14541)
                     /gene="rlmN"
                     /locus_tag="ACHKW0_000292"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HFO7473187.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="ACHKW0_000293"
     CDS             complement(14691..15122)
                     /gene="ndk"
                     /locus_tag="ACHKW0_000293"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HFO7473188.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15271..17583)
                     /gene="pbpC"
                     /locus_tag="ACHKW0_000294"
     CDS             complement(15271..17583)
                     /gene="pbpC"
                     /locus_tag="ACHKW0_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HFO7473189.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPDQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     SYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17584..22545)
                     /gene="yfhM"
                     /locus_tag="ACHKW0_000295"
     CDS             complement(17584..22545)
                     /gene="yfhM"
                     /locus_tag="ACHKW0_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HFO7473190.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLYLLEENKLLPDEQNTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRVDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22752..23597
                     /gene="sseA"
                     /locus_tag="ACHKW0_000296"
     CDS             22752..23597
                     /gene="sseA"
                     /locus_tag="ACHKW0_000296"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HFO7473191.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            24259..24384
                     /gene="timP"
                     /locus_tag="ACHKW0_000297"
     CDS             24259..24384
                     /gene="timP"
                     /locus_tag="ACHKW0_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small toxic inner membrane protein TimP"
                     /protein_id="HFO7473192.1"
                     /translation="MKIRCFCIVLIVSGALFSQVNNNRSLSGDNLLVVNNLQSSK"
     gene            complement(24416..25192)
                     /gene="sseB"
                     /locus_tag="ACHKW0_000298"
     CDS             complement(24416..25192)
                     /gene="sseB"
                     /locus_tag="ACHKW0_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HFO7473193.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGGEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(25334..26617)
                     /gene="pepB"
                     /locus_tag="ACHKW0_000299"
     CDS             complement(25334..26617)
                     /gene="pepB"
                     /locus_tag="ACHKW0_000299"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HFO7473194.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPELIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26795..26995)
                     /gene="iscX"
                     /locus_tag="ACHKW0_000300"
     CDS             complement(26795..26995)
                     /gene="iscX"
                     /locus_tag="ACHKW0_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HFO7473195.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(27007..27342)
                     /gene="fdx"
                     /locus_tag="ACHKW0_000301"
     CDS             complement(27007..27342)
                     /gene="fdx"
                     /locus_tag="ACHKW0_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HFO7473196.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27344..29194)
                     /gene="hscA"
                     /locus_tag="ACHKW0_000302"
     CDS             complement(27344..29194)
                     /gene="hscA"
                     /locus_tag="ACHKW0_000302"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HFO7473197.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(29211..29726)
                     /gene="hscB"
                     /locus_tag="ACHKW0_000303"
     CDS             complement(29211..29726)
                     /gene="hscB"
                     /locus_tag="ACHKW0_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0097428 - protein maturation by iron-sulfur
                     cluster transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HFO7473198.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29822..30145)
                     /gene="iscA"
                     /locus_tag="ACHKW0_000304"
     CDS             complement(29822..30145)
                     /gene="iscA"
                     /locus_tag="ACHKW0_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HFO7473199.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(30162..30548)
                     /gene="iscU"
                     /locus_tag="ACHKW0_000305"
     CDS             complement(30162..30548)
                     /gene="iscU"
                     /locus_tag="ACHKW0_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HFO7473200.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30576..31790)
                     /gene="iscS"
                     /locus_tag="ACHKW0_000306"
     CDS             complement(30576..31790)
                     /gene="iscS"
                     /locus_tag="ACHKW0_000306"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HFO7473201.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31902..32390)
                     /gene="iscR"
                     /locus_tag="ACHKW0_000307"
     CDS             complement(31902..32390)
                     /gene="iscR"
                     /locus_tag="ACHKW0_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HFO7473202.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(32660..33400)
                     /gene="trmJ"
                     /locus_tag="ACHKW0_000308"
     CDS             complement(32660..33400)
                     /gene="trmJ"
                     /locus_tag="ACHKW0_000308"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HFO7473203.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            33519..34322
                     /gene="suhB"
                     /locus_tag="ACHKW0_000309"
     CDS             33519..34322
                     /gene="suhB"
                     /locus_tag="ACHKW0_000309"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0046854 - phosphatidylinositol phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HFO7473204.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34467..35321
                     /gene="yfhR"
                     /locus_tag="ACHKW0_000310"
     CDS             34467..35321
                     /gene="yfhR"
                     /locus_tag="ACHKW0_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7473205.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35512..36792
                     /gene="csiE"
                     /locus_tag="ACHKW0_000311"
     CDS             35512..36792
                     /gene="csiE"
                     /locus_tag="ACHKW0_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HFO7473206.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISETGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36784..37923)
                     /gene="hcaT"
                     /locus_tag="ACHKW0_000312"
     CDS             complement(36784..37923)
                     /gene="hcaT"
                     /locus_tag="ACHKW0_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HFO7473207.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGESRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(38083..38973)
                     /gene="hcaR"
                     /locus_tag="ACHKW0_000313"
     CDS             complement(38083..38973)
                     /gene="hcaR"
                     /locus_tag="ACHKW0_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HFO7473208.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLANVTA"
     gene            39109..40470
                     /gene="hcaE"
                     /locus_tag="ACHKW0_000314"
     CDS             39109..40470
                     /gene="hcaE"
                     /locus_tag="ACHKW0_000314"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit alpha"
                     /protein_id="HFO7473209.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40467..40985
                     /gene="hcaF"
                     /locus_tag="ACHKW0_000315"
     CDS             40467..40985
                     /gene="hcaF"
                     /locus_tag="ACHKW0_000315"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008695 - 3-phenylpropionate dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019380 - 3-phenylpropionate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HFO7473210.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40985..41305
                     /gene="hcaC"
                     /locus_tag="ACHKW0_000316"
     CDS             40985..41305
                     /gene="hcaC"
                     /locus_tag="ACHKW0_000316"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HFO7473211.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41302..42114
                     /gene="hcaB"
                     /locus_tag="ACHKW0_000317"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41302..42114
                     /gene="hcaB"
                     /locus_tag="ACHKW0_000317"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HFO7473212.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            42124..43326
                     /gene="hcaD"
                     /locus_tag="ACHKW0_000318"
     CDS             42124..43326
                     /gene="hcaD"
                     /locus_tag="ACHKW0_000318"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HFO7473213.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43423..43845
                     /gene="yphA"
                     /locus_tag="ACHKW0_000319"
                     /pseudo
     CDS             43423..43845
                     /gene="yphA"
                     /locus_tag="ACHKW0_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
     gene            complement(43893..44765)
                     /gene="yphB"
                     /locus_tag="ACHKW0_000320"
     CDS             complement(43893..44765)
                     /gene="yphB"
                     /locus_tag="ACHKW0_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HFO7473214.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVALL"
     gene            complement(44777..45871)
                     /gene="yphC"
                     /locus_tag="ACHKW0_000321"
     CDS             complement(44777..45871)
                     /gene="yphC"
                     /locus_tag="ACHKW0_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7473215.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45904..46902)
                     /gene="yphD"
                     /locus_tag="ACHKW0_000322"
     CDS             complement(45904..46902)
                     /gene="yphD"
                     /locus_tag="ACHKW0_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7473216.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46927..48438)
                     /gene="yphE"
                     /locus_tag="ACHKW0_000323"
     CDS             complement(46927..48438)
                     /gene="yphE"
                     /locus_tag="ACHKW0_000323"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO7473217.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48461..49444)
                     /gene="yphF"
                     /locus_tag="ACHKW0_000324"
     CDS             complement(48461..49444)
                     /gene="yphF"
                     /locus_tag="ACHKW0_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HFO7473218.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKIY
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49541..52822)
                     /gene="yphG"
                     /locus_tag="ACHKW0_000325"
     CDS             complement(49541..52822)
                     /gene="yphG"
                     /locus_tag="ACHKW0_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HFO7473219.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLAAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVVLDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARL
                     QRAKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     AAEMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQRCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEATRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQIAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52940..54133
                     /gene="yphH"
                     /locus_tag="ACHKW0_000326"
     CDS             52940..54133
                     /gene="yphH"
                     /locus_tag="ACHKW0_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HFO7473220.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPGRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(54197..55450)
                     /gene="glyA"
                     /locus_tag="ACHKW0_000327"
     CDS             complement(54197..55450)
                     /gene="glyA"
                     /locus_tag="ACHKW0_000327"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HFO7473221.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55778..56968
                     /gene="hmpA"
                     /locus_tag="ACHKW0_000328"
     CDS             55778..56968
                     /gene="hmpA"
                     /locus_tag="ACHKW0_000328"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008941 - nitric oxide dioxygenase NAD(P)H
                     activity [Evidence IEA];
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0051409 - response to nitrosative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HFO7473222.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKQEGAFSDPTMQFYLCGPVGFMQFAAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(57013..57351)
                     /gene="glnB"
                     /locus_tag="ACHKW0_000329"
     CDS             complement(57013..57351)
                     /gene="glnB"
                     /locus_tag="ACHKW0_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HFO7473223.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57412..58746)
                     /gene="glrR"
                     /locus_tag="ACHKW0_000330"
     CDS             complement(57412..58746)
                     /gene="glrR"
                     /locus_tag="ACHKW0_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HFO7473224.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58736..59449)
                     /gene="qseG"
                     /locus_tag="ACHKW0_000331"
     CDS             complement(58736..59449)
                     /gene="qseG"
                     /locus_tag="ACHKW0_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HFO7473225.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(59614..61041)
                     /gene="qseE"
                     /locus_tag="ACHKW0_000332"
     CDS             complement(59614..61041)
                     /gene="qseE"
                     /locus_tag="ACHKW0_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HFO7473226.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61616..65503)
                     /gene="purL"
                     /locus_tag="ACHKW0_000333"
                     /gene_synonym="purI"
     CDS             complement(61616..65503)
                     /gene="purL"
                     /locus_tag="ACHKW0_000333"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HFO7473227.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65761..67317
                     /gene="mltF"
                     /locus_tag="ACHKW0_000334"
     CDS             65761..67317
                     /gene="mltF"
                     /locus_tag="ACHKW0_000334"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HFO7473228.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67314..67817)
                     /gene="tadA"
                     /locus_tag="ACHKW0_000335"
     CDS             complement(67314..67817)
                     /gene="tadA"
                     /locus_tag="ACHKW0_000335"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HFO7473229.1"
                     /translation="MSEVEFSHEYWMRHALTLAKRAWDEREVPVGAVLVHNNRVIGEG
                     WNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCAGAMIHSRIGRV
                     VFGAHDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFFRMRRQEIKAQK
                     KAQSSTD"
     gene            complement(67875..68510)
                     /gene="yfhb"
                     /locus_tag="ACHKW0_000336"
     CDS             complement(67875..68510)
                     /gene="yfhb"
                     /locus_tag="ACHKW0_000336"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HFO7473230.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68719..69567
                     /gene="yfhH"
                     /locus_tag="ACHKW0_000337"
     CDS             68719..69567
                     /gene="yfhH"
                     /locus_tag="ACHKW0_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HFO7473231.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRQSEA
                     LMKKLV"
     gene            69623..69883
                     /gene="yfhL"
                     /locus_tag="ACHKW0_000338"
     CDS             69623..69883
                     /gene="yfhL"
                     /locus_tag="ACHKW0_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HFO7473232.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(70077..70157)
                     /gene="shoB"
                     /locus_tag="ACHKW0_000339"
     CDS             complement(70077..70157)
                     /gene="shoB"
                     /locus_tag="ACHKW0_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HFO7473233.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70577..70957)
                     /gene="acpS"
                     /locus_tag="ACHKW0_000340"
     CDS             complement(70577..70957)
                     /gene="acpS"
                     /locus_tag="ACHKW0_000340"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HFO7473234.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70957..71688)
                     /gene="pdxJ"
                     /locus_tag="ACHKW0_000341"
     CDS             complement(70957..71688)
                     /gene="pdxJ"
                     /locus_tag="ACHKW0_000341"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HFO7473235.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71700..72428)
                     /gene="recO"
                     /locus_tag="ACHKW0_000342"
     CDS             complement(71700..72428)
                     /gene="recO"
                     /locus_tag="ACHKW0_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HFO7473236.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72440..73345)
                     /gene="era"
                     /locus_tag="ACHKW0_000343"
     CDS             complement(72440..73345)
                     /gene="era"
                     /locus_tag="ACHKW0_000343"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HFO7473237.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73342..74022)
                     /gene="rnc"
                     /locus_tag="ACHKW0_000344"
     CDS             complement(73342..74022)
                     /gene="rnc"
                     /locus_tag="ACHKW0_000344"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HFO7473238.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(74295..75269)
                     /gene="lepB"
                     /locus_tag="ACHKW0_000345"
     CDS             complement(74295..75269)
                     /gene="lepB"
                     /locus_tag="ACHKW0_000345"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HFO7473239.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(75285..77084)
                     /gene="lepA"
                     /locus_tag="ACHKW0_000346"
     CDS             complement(75285..77084)
                     /gene="lepA"
                     /locus_tag="ACHKW0_000346"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HFO7473240.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(77282..77761)
                     /gene="rseC"
                     /locus_tag="ACHKW0_000347"
     CDS             complement(77282..77761)
                     /gene="rseC"
                     /locus_tag="ACHKW0_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HFO7473241.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77758..78714)
                     /gene="rseB"
                     /locus_tag="ACHKW0_000348"
     CDS             complement(77758..78714)
                     /gene="rseB"
                     /locus_tag="ACHKW0_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HFO7473242.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78714..79364)
                     /gene="rseA"
                     /locus_tag="ACHKW0_000349"
     CDS             complement(78714..79364)
                     /gene="rseA"
                     /locus_tag="ACHKW0_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HFO7473243.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79397..79972)
                     /gene="rpoE"
                     /locus_tag="ACHKW0_000350"
     CDS             complement(79397..79972)
                     /gene="rpoE"
                     /locus_tag="ACHKW0_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HFO7473244.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79969..80124)
                     /gene="rseD"
                     /locus_tag="ACHKW0_000351"
     CDS             complement(79969..80124)
                     /gene="rseD"
                     /locus_tag="ACHKW0_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HFO7473245.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80380..82002
                     /gene="nadB"
                     /locus_tag="ACHKW0_000352"
     CDS             80380..82002
                     /gene="nadB"
                     /locus_tag="ACHKW0_000352"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HFO7473246.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81987..82724)
                     /gene="trmN"
                     /locus_tag="ACHKW0_000353"
     CDS             complement(81987..82724)
                     /gene="trmN"
                     /locus_tag="ACHKW0_000353"
                     /EC_number="2.1.1.223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HFO7473247.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82856..84190
                     /gene="srmB"
                     /locus_tag="ACHKW0_000354"
     CDS             82856..84190
                     /gene="srmB"
                     /locus_tag="ACHKW0_000354"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HFO7473248.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
BASE COUNT        20085 a        23077 c        21165 g        19913 t
ORIGIN      
        1 tgatgatctt aaatgtctta tttttcgtaa tgtgtataac aaggaatagt gatgaaattt
       61 aaaaaatgtc ttctgcctgt agcaatgtta gcgtcattca ctctggcagg atgccagtca
      121 aatgctgacg atcatgccgc cgatgtttat caaaccgatc aactgaatac caaacaagaa
      181 actaaaaccg ttaatattat ttccattctt cccgcaaaag ttgccgtaga caactcccaa
      241 aataaacgga acgcacaagc cttcggcgcg cttattggcg cagtcgctgg cggtgttatc
      301 ggccacaacg tcggttctgg cagcaattcc ggaacgacgg caggggcagt tggcggcgga
      361 gctgtaggcg cggcagcggg ttctatggtg aatgataaaa ccttagtgga aggtgtttct
      421 ttaacgtata aggaaggcac caaagtgtat acctccaccc aggtgggtaa agagtgccag
      481 tttacgacag gtttagccgt tgttattacc acgacgtata acgaaacgcg tattcagcca
      541 aatactaaat gtcctgaaaa gagctaataa tcaggaggag tcatgaagaa agtttttctt
      601 tgcgccatct tggcctcctt aagctatccg gctatcgcct catcattgca ggatcaactc
      661 tcggctgtcg cagaagcgga acagcaaggt aaaaatgaag agcaaaagca gcatgacgaa
      721 tgggtcgcgg agcgcaacag ggaaatccag caagagaagc aacgtcgcgc aaatgcccag
      781 gccgccgcta acaaaagagc ggcaacggca gcagcaaata agaaagctcg tcaggataaa
      841 ctggacgccg aagcctctgc ggacaaaaaa cgcgatcaaa gttatgaaga tgagctacgc
      901 agcttagaga ttcagaaaca aaaactggcg ctggcaaaag aagaagcccg cgttaagcga
      961 gaaaacgaat ttatcgatca ggaactgaag cacaaagctg cgcaaaccga tgtggtgcaa
     1021 tctgaagctg acgccaacag aaatatgact gaaggcggtc gcgatctgat gaaaagcgtg
     1081 ggcaaagcag aagagaacaa atcggacagc tggtttaatt aatcgatgtt agtaacttca
     1141 agcctatgat tcttgaagat aaaaaaccct ctgtagtaac agagggtttt gttcattcat
     1201 agtgcaggga ttaaaatcat tcccactcaa tggtagctgg cggcttgccg ctgatgtcat
     1261 acaccacgcg ggaaataccg ttcacttcat tgataatgcg gttggaaacg cgaccgagga
     1321 aatcgtacgg cagatgcgcc cagtgtgcgg tcataaagtc gatggtttcg acagcacgca
     1381 gagagacaac ccagtcatac ttacgaccat cgcccattac gccaacggaa cgtaccggca
     1441 ggaacacagt gaacgcctgg ctgactttgt cgtacaggtc cgctttacgc agttcttcaa
     1501 tgaagatggc gtcagcacgg cgcagcaggt cacagtactc tttcttcact tcacccagca
     1561 cacgaacgcc aaggcctggt cccgggaacg ggtgacggta cagcatgtcg tacggcaggc
     1621 ccagctccag accaatctta cgcacttcgt ctttgaacag ctctttcagc ggttcaacca
     1681 ggcccatctt catctctttc ggcaggccgc ccacgttgtg gtgagatttg atgacgtgtg
     1741 ctttaccggt tgcagaagcc gcagattcga taacgtcagg gtagatggtg ccctgcgcca
     1801 gccacttcac gtcttccagt ttcagcgctt cttcatcgaa tacttcaacg aaaacgcgcc
     1861 cgatgatttt acgttttgct tccggatcgt tttcgccagc cagcgctgac aggaagcgat
     1921 cttctgccgg tacgtgaaca atgttaagac caaagtgatc gccaaacata tccagaacct
     1981 gctctgcttc gttgagacgc agcaggccgt tgtcgacgaa tacgcaagtc aggtttttac
     2041 cgatagcgcg gtgcagcagc attgcggtta cggaggaatc cacaccacca gagaggccga
     2101 ggatgacttt atcgtcgcct acctgctcgc ggatgcgagc tacagcatcg tcgataattt
     2161 tcgctggcgt ccacagggct tcacactggc agatatcacg cacaaaacgc tccagcatgc
     2221 gcataccctg gcgggtgtga gtcacttccg ggtggaactg tacgccatag aagcgttttt
     2281 cttcgttagc cataatggca aacgggcagc tttcggtgct ggctacggtg acgaagtcgg
     2341 acgggatagc ggtaacttta tcgccgtggc tcatccagac atcgagcagc ggtttaccgt
     2401 ctgcggtcag cgcatcttcg ataccgcgaa ccagtgcgct gtcgtttacg acttcaacct
     2461 gcgcgtagcc aaattcacgt tcgttagagg cttcaacgtg accgcccaac tgcattgcca
     2521 tggtctgcat gccatagcaa acgccgaata ccggtacgcc tgcttcaaag acatactgcg
     2581 gcgcacgcgg gctgttttct tcggtggtgc tttccgggcc gccggaaaga ataatgccgc
     2641 ttggattgaa gtcacgaatt tgtgcttctg tcacatccca cgcccacagt tcgcagtaaa
     2701 cacccagctc acgcacgcgg cgcgcaacca gttgagtgta ctgagaaccg aagtccagaa
     2761 tgaggatgcg atgcttatga atgttttccg tcattgacgc ttattccgag gcaagtgaaa
     2821 cagataatat aaatcgcccg acatgaagtc gggcgaagag aatcaggagc ccagacggta
     2881 gttcggggac tctttagtaa tggtcacgtc gtgaacgtgg ctttcctgaa tgcccgcacc
     2941 gctgatacgt acaaactccg ctttagtacg cagttcgtcg atagtaccac agccggtcag
     3001 acccatacag gagcgcaggc cgcccatctg ctggtgaatg atctctttca ggcgaccttt
     3061 ataagctacg cgaccttcga taccttccgg caccagtttg tcggcagcgt tatcgctctg
     3121 gaaataacgg tcagaggaac ctttggacat cgcgcccagg gaacccatac cacggtaaga
     3181 tttgtaagaa cggccctggt agagttcgat ttcacccgga gattcttcag tacccgccag
     3241 catggaacct accatcaccg cgcttgcgcc agctgcgata gctttggcga tgtcgccgga
     3301 gaagcgaata ccaccatctg cgataaccgg aataccggtg ccttccaggg cttcaactgc
     3361 gtcagcaacg gcagtgatct gcggaacacc tacgccagta acgatacgag tcgtacagat
     3421 agaaccaggg ccgataccga ctttaaccgc gctgcaacca gcttctgcca aagcgcgtgc
     3481 acctgcagct gttgccacgt tgccgccgat gatttgcaga tccggatatt tagcacgggt
     3541 ttcacggata cgctgcaaaa ctccttctga gtgaccgtgg gaggagtcga tcagcagaac
     3601 gtcaacgcca gcggcaacca gcgcgtcaac acgctcttcg ttacccgcac ctgcgccaac
     3661 tgcagcacca acacgcagac ggccttgctc gtctttacag gcgttcggtt tacgttccgc
     3721 tttctggaag tctttcacgg tgatcatgcc gatcaggtgg aattcgtcat caaccaccag
     3781 cgctttttca acgcgttttt cgtgcatttt tgccagcacc acttcacggg cttcaccttc
     3841 acgcacggtg accagacgct ctttcggcgt catgtaaacg ctaaccggct ggttcaggtc
     3901 ggtaacaaaa cgcacgtcac gaccggtgat aatacccacc agttcgtttt cttcggtaac
     3961 gaccggatag cccgcaaaac cgttacgctc ggtcagttct ttcacttcgc gcagcgtcgt
     4021 ggttggcaga acagtctgcg gatcagtcac cacaccagat tcgtgttttt tcacacggcg
     4081 aacttcttct gcctggcgtt caatggacat gtttttgtgg ataaagccga taccgccttc
     4141 ctgagccaga gcaatagcca ggcgcgcttc cgttacggta tccattgctg cggaaagcat
     4201 agggatattc agacgaatag ttttcgtcag ctgggtgctg aggtcagcag tattcggcag
     4261 aacggtagaa tgagcaggaa cgaggagaac gtcgtcaaac gtcagagctt ctttagcgat
     4321 acgtagcatg ggcaatatct cgaccagagt ggttaataaa tattgccgcg gcattataca
     4381 gagcgtaacc gattgcatct accccttttt gcaaaaaatg cttgctatcc ccgaagggcg
     4441 ggttactatc gactgaataa cctgctgatt ttgaatttga tctcgctcac atgttacctt
     4501 ctcaatcccc tgcaattttt accgttagtc gcctgaatca aacggttcgt ctgctgcttg
     4561 agcatgagat gggacaggtt tggatcagcg gcgaaatctc taatttcaca caaccggctt
     4621 ccggtcactg gtactttaca ctcaaagacg acaccgccca ggtacgctgc gcgatgttcc
     4681 gcaacagcaa ccgtcgggtg accttccgtc cacagcatgg gcaacaagtt ttagttcgcg
     4741 ccaatattac gctctacgag ccgcgcggcg actaccagat aatcgttgag agtatgcagc
     4801 cggccggtga agggctgctg caacagaagt acgaacagct caaagcgaag ttgcaggctg
     4861 aaggattgtt cgatctgcaa tacaaaaaac cacttccctc ccctgcgcat tgcgttggtg
     4921 tgatcacctc aaaaaccggt gctgcgctac atgatatttt gcatgtgtta aaacgtcgcg
     4981 atccttctct accggtgatc atctacccca ccgccgttca gggcgatgac gcgccggggc
     5041 aaactgttcg cgccattgaa ctggcgaatc agcgcaatga gtgcgacgtg ttgatcgttg
     5101 ggcgcggcgg cggttcgctg gaagatttat ggagttttaa cgacgaacgc gtagcgcggg
     5161 cgatttttgc cagccgcatt ccggtcgtca gcgccgtcgg gcatgagacg gatgtgacca
     5221 ttgccgattt tgttgccgat ctgcgtgcgc caacgccgtc tgccgccgct gaagtagtga
     5281 gccgtaatca gcaagagtta ctgcgccagg tgcaatcgac ccgtcaacgg ctggagatgg
     5341 cgatggatta ttatctcgcc aaccgcacgc gtcgctttac gcagatccat caccgattac
     5401 agcaacagca tccgcagctc cggctggcac gccagcaaac catgcttgag cgcctgcaaa
     5461 agcggatgag ctttgcgctg gaaaatcaac ttaagcgtac cgggcaacag cagcagcggt
     5521 taacacagcg gctgaatcag caaaatccac agccgaagat tcatcgcgcg caaacgcgca
     5581 ttcagcaact ggaatatcgt ttagcagaaa ccctgcgcgt acagcttagc gccacgcgtg
     5641 aacgtttcgg taatgcagta acgcacctcg aagccgtaag cccactgtca acgctggcgc
     5701 gtggatacag cgttactact gctactgacg gcaatgtact gaaaaaagtg aagcaagtta
     5761 aagcgggtga aatgctaacc acacgtctgg aagacggctg gatagaaagt gaagtaaaaa
     5821 acatccagcc ggtaaaaaaa tcgcgtaaaa aggtgcatta agccagcacg aattcaacgc
     5881 gttttttcga tatcaagcca tgcccgtgct gacagaagta atctactgca ccgcaggctt
     5941 ttaacacctg caacggttga tggcagtctg gacaaagagc tttcatttca ataatttccc
     6001 cgcaggatgg gcaacgcgca tggccgttat cctgatcaag cacatgctga cattgtggac
     6061 aatgcagttc catatgaatt cctccggtag ccatccctct acattcatag agggaatggc
     6121 agataaaata cttacggata attatttatt tttcttgatg tgcttcatca gacgcttacg
     6181 tttacgcatc tgggttggcg tcagggtgtt acgcttgttc gcatacgggt tttccccttc
     6241 tttgaactga atacgaatcg gcgatcccat tacgtccagc gatttgcgga agtagttcat
     6301 caagtagcgc ttgtaggaat caggcaggtc tttcacctga ttaccgtgaa tcaccacaat
     6361 cggcgggtta taaccaccag cgtgggcata tttcagcttc acacgacgac cgcgtaccag
     6421 cggcggttgg tgatcttcaa cagccatcgt catgatgcgc gtcagcatag aggtccccac
     6481 acgacgggtg gagctgtcat acgcttcacg tactgattca aacaagttac caacaccact
     6541 gccgtgcaag gcagagataa agtgcacacg agcaaaatcg ataaagccca gacggaagtc
     6601 cagcgtttct ttcacctgct ctttcacttc ctggctcagg ccatcccact tattcaccac
     6661 aatgacaagt gaacgcccac tattgagaat aaagcccagc agcgagagat cctgatcgga
     6721 aataccttcg cgcgcatcaa tcactaacat caccacgttg gcatcttcaa tggcctgcaa
     6781 cgttttgatt acggagaatt tctctacagc atcggtgatt ttgccgcgtt tacgtacgcc
     6841 agcggtgtca atgagcacat actcacgtcc atcgcgttcc attggaatgt agatactgtc
     6901 acgcgtcgtg ccaggcatgt cataaacaac aacgcgctct tcaccaagaa tacggttagt
     6961 gagtgtagac ttacctacgt tcggacgacc cacaatcgcc agtttgatcg gcagactttg
     7021 cgggtcgaag tcgtcttcct cttcttcttc gccgttctct tccgcttcaa attgcgccca
     7081 gtattcagcg tcttcgtcga cttcctcttg cggtgcgaga tcttccatcc acggcagcag
     7141 cacatgctcc agcagactta atacgccacg accgtgagac gcggcgatcg ggtagatttc
     7201 acctaaacca agcgagtaga aatcaaccac tgcctgatcg ggatccagac cgtcagtttt
     7261 gtttgccacc aggaaggtcg gtttttcacg ggagcgcaga tgtttggcaa tcgcttcatc
     7321 tgccggcatc aggcccgcgc gcgcatccac cataaacagt acgacgtccg cttcttcaat
     7381 cgccagcagc gactgttccg ccatgcgggt ttctacaccg tcttctgtgc catcaatccc
     7441 gccggtatca atacagataa actcacggcc ttcaatttcc gcacgaccgt acttacggtc
     7501 acgagtcaga cccgggaaat ccgcaaccag cgcatctcgg gtgcgagtta gacggttaaa
     7561 taacgtggat tttcctacgt tagggcgccc gacaagcgcg accacaggta ccatgtttaa
     7621 agcctcattt ttataaatca tcagacaacg cacgctatat tcgcgtcgtt gttaaaaaca
     7681 ggaaaacggc ccctgtccag gagccgtttt caaagtgaac gacagagacg attaacgtgt
     7741 aatagagtac acggttccgt cttttgcctg gatcagcagt ttgccgtcag cggcaaccgg
     7801 ttcagtctgg aaaccggaac tatcaacttt ttgctgggca acgaaacgac catcttcgac
     7861 gttaatccag tgcagataac cttcactgtc accgaccacc aggttgccat tatacagcac
     7921 cggagaagtc agcaggcgat gcagcagatc gctttgtgcc cacagcgtaa cgccgccatc
     7981 aatggtcaac gccatcaccc ggtcattttg atcgaccaga tagatgcgat tgccgtcgac
     8041 gatgaaatca ttcaccgaac ccagttcgcg tttccacata atctgaccac tgcgcagatc
     8101 aagcgccgtc aggttaccat tataggccag cgcgaaaaca acgccgttaa caacgacggg
     8161 agtcgtgtca acatcgctca gacggtcaat ttcggtagaa ccggtcgcct gggaaatacg
     8221 ctgctgccaa atcatctggc cctgttccat cagcactgcg ctgacgcgac cattatcgcc
     8281 ccccacgacg gccgcaccaa aagccgttgc cggcgcagac tcgccacgca aagagagcga
     8341 aggcatatca aggttaactg tccatttgac agcgccgtca gcttcgttca gcgcttgtaa
     8401 ctgaccgtta ctggtgtgga ttaacaccag accgtcgctg accaccgggc gcgaaagtgc
     8461 ttcacccgcg actttagttt gccatgccac agtaccatcg ctggtattca gcgcgtaaac
     8521 ctgcgccttt tcgctgccaa tgtagacatg cccaccagac acggtcacac cgccagaaag
     8581 taatgcaggc tctttagaga accagccatc tttctcggcc aggctgacag accagatttc
     8641 tttgccatca tccgcattca gcgcttttac caaaccagcg cggtccgctg catagacaac
     8701 gttgtccgcc agtgccggat gaagattgga atagaagttg ccaatgccgc taccaacgga
     8761 agtactccac gccgtggtcg gcgtaaactg gttttcaacg gttggcaatg gggacatctt
     8821 taccacatct tcttcgctgt taaacagcga acagccgctt aaaagggtaa cggaaagcag
     8881 tcctggcagc agtaatttac gcaattgcat cgggtccctc tcagatggac aaattattaa
     8941 ttttcatctg catcatttcg ctcagtgccg gagtaacatc gcttttcacg cctgcttccc
     9001 atgcactacg cgcaccttgc ttatcacctt tgctcagcaa tgcttcacca cgcaggtcgg
     9061 caacaatggc agcccaccct tcacctttga tggtatcaag ggttttcagc gcggcatcag
     9121 cctgcttgag ctgtacctga acgcgagcaa gacgcagatt tatcacggct ttgagatttt
     9181 catcgctcgt gtctgccagc ccctgttgta actgggcggc agctttctct agttcatttt
     9241 tgtcaacaaa ttgctgcgca agttccaaag aagccagcgc accataagta tttttatttt
     9301 cagcagcaaa tttttccgcc gccgggatgc tatccggttt gccttcgctc actgcggtaa
     9361 ccgcattttg ataggcaaga gaagcggagc gtgcagaatc aacctgatgg ctgttccagt
     9421 agcgccagcc aatcagtgcg ccaacgccca aaatcacccc aacagccagt gctttgccat
     9481 tttcagcaaa aaagcgttta accgcttcta cctggtcgtt ttcgttctcg taaatttcca
     9541 cgctgtcctt ctccttcctt aacccagtaa cgtgcgcaag tgcgcggcta cgctatcctg
     9601 cgcaactgcc gtttgctcac cagagcgcaa atccttcact actgctgtgc cgttagccac
     9661 ttcagactca cccagcacca cagcaacgcg ggcaccccat ttatcagcac gggcaaactg
     9721 tttcttaaag ttgccgccgc cgtggttggt catcaatttc acgcccggta attcatcacg
     9781 cagacgctca gctaatgcca tagccgcaga ttgtgtatca gcacctgaag ccaccaggta
     9841 tatatcgaca acaggatcgg ctttaaattc cggattaacg gcctgtacta acaatacaag
     9901 acgttcgagg cccatcgcaa aaccgacagc cggtgttgca cgaccgccca gttgttccac
     9961 aagaccgtca taacgaccgc ctgcacacac ggtgccctgg gagccgagac tgttagtcac
    10021 ccactcgaaa acggtacggt tatagtaatc cagaccacgc accagacgct ggtttacggt
    10081 gtaagcgatc cccgcgctct cgagcagttt gcacagaccg gcaaaatgct cacgagattc
    10141 ctcgtccaga taatcaccta atgccggagc gtcgttgaga agcgcctgca cttccggatt
    10201 ttttgaatcc agcacgcgca gcgggttagt gtacatgcgg cgtttgcagt cttcgtccag
    10261 cttttcttta tgctgctcaa ggaatgccac cagcgcatcg cggtaattgg cgcgtgcttc
    10321 cagcgaaccg atagaattca gctcaagagt tacgtgttcg gaaataccca gcgcgcgcca
    10381 ccagcgggcg gtgagcataa tcagttcagc gtcgatatcc ggaccttgca gaccgaaaac
    10441 ttcgcagccc aactgatgga actgacgata acgccctttc tgcggacgct cgtgacggaa
    10501 catcggcccg atataccaca gacgctgttc ctgattgtac agaagaccat gctcgatgcc
    10561 ggcgcgtaca cagcccgccg tcccttcagg gcgcagagtc aggctgtcgc cattgcgatc
    10621 ctcaaaggtg tacatctctt tttcaaccac gtcggtgact tcaccaatcg cacgtttgaa
    10681 tagcggggtc tgctctacaa tcggcaagcg gatttcactg taaccgtagc tgccgagcac
    10741 gtttttcagt gtgccttcaa tgcgctgcca gatggccgtt tcgccaggca ggtaatcgtt
    10801 catgccgcga atggcttgaa tgttttttgc cacgtttatt ctctttctga atataaaaat
    10861 gaaccctcaa cgcttccctc aatgtttcgg gagctatgcg ggttcaatca tacacgggaa
    10921 gcgaggcgct tcccatcacg ttattatttt tcaacctgct gaacgtcaat tcgacgcgct
    10981 tcgtccagct gactggcttt cgcacgaatg cgtgcttcca gctggtcgat catatcgttg
    11041 ttgtccagac ggtctttgcg cacgccatct tcatagaggc cgcttttctt gttgccgccg
    11101 gtgacgccga gtgtagaaac cagcgcctca cctgggccat tcaccacgca gccgataatc
    11161 gaaacgtcca tcggagtgat gatatcttcc aggcgttgct ccagcgcgtt aaccgtaccg
    11221 ataacatcaa attcctgacg cgaacaggtc gggcaggcga tgaagttgat cccgcgcgaa
    11281 cggatacgca gcgatttcaa aatatcgaaa ccgactttga tctcttcgac cggatcggcc
    11341 gccagtgata cgcgcagcgt gtcgccgatg ccttcagaca gcagcagacc taaaccaatg
    11401 gcggatttta ctgccccgct gcgtgcacca ccggcttcgg tgatccccag atgcagcggc
    11461 tgatcgatct gttttgccag caaacgataa gactcaacag cgaggaagac gtcagacgct
    11521 ttcacgctga ctttgaactg atcgaagttc aggcgatcga gatgatcaac atggcgcatg
    11581 gcggattcca gcaacgcctg cggcgtcggt tcgccatact tttcttgcag atctttttcc
    11641 agcgatccgg cgttaacgcc aatacggatc ggaatgtttt tatcgcgcgc acagtcaacc
    11701 accatgcgaa tacgctcttc attaccgata ttgccagggt taatacgcag acaatcgacg
    11761 ccgtattccg ctactttcag tgcaatgcga tagtcgaagt ggatgtcagc caccagcggc
    11821 acgttaacct gctgtttgat gagtttgaac gcttctgccg cgtccatcgt cggtacggag
    11881 acacggacga tatcagcgcc aacgcgttcc agcgccttga tttgattgac cgttgcttcg
    11941 acgtctgtcg tacgcgtatt ggtcatggac tgtacggcga tgggagcacc atcgccaatc
    12001 ggcacattcc caacgtaaat acgtgttgat tttctacgtt gaattggagc ctggttatgc
    12061 atgaaaaatc tcccgcgtta cccgtctgtt actgcgccgg tgattgttcg gcattgaggg
    12121 tcagacgcgc aacctggtta gttctgataa aacgactcag atcgacaggt ttcccttgat
    12181 actggatctg tactgcggct ggcgcaccaa ttttcagttt gtatggtgcc tggccggtta
    12241 agttcaaatt accgtcttta cgctgcatac cgctaaacaa ttttttaccg gtagcatcag
    12301 taacctccag ccagcaatcg gcagtaaagt tcatcaccag cgcattcgga tcagccgccg
    12361 gcgtagtcac gcctgcctga tcggttggca acggcgcagc gccatctggc gttgttgttg
    12421 ccgttggtgc cggggtcgcg gcggtatcaa catttgcctg cgaaggcgaa acaaccgcat
    12481 tctgttgcgg atcaacagct ggtgctggcg cagttacggc aggtgtttgc gtgttggttg
    12541 cggtagtatc cacagacgct ggcggcgtgg acgttgtagc cgggtctgta gttgtcgacg
    12601 tatttaacgg aacactctgc ccctgctcgc tattactgct cagttccgcc gaagattgat
    12661 cggccatagt ggtgatctct tcctgctgag ctttgtggtc ttgccaccac caggcaccgc
    12721 tcaggccgat aaccacaaac aacaccagcc aggtgaaggt catcagccag ccgtcacgtt
    12781 ttttgcggcg tttaccgagg gaaaaactct gcataggcgc aacttttgca gcccgtagtg
    12841 gagcctgctt ttccagccct ggcagcagtt cttcttctgg aatatgtacc agacgcgcat
    12901 aagagcggat atatccgcgc aggaatgttg aagcaagatc ggcgggtgcc ttatcttctt
    12961 caatgtcgcg taccgtggaa accttcaggc aaagtcgctc ggcaacggcc tgctgactaa
    13021 gtcctagttg ttcgcgagca ttacgcaggc gagcgccggt agtaagtgct tcattttggt
    13081 cgtgcgtggc ttcagtattc attcgctgca ggtacgttta aatgagaatt gagatgccgg
    13141 tgaaccatca tgctcaccca caccgcgaaa catccgttaa gttaaccgtt atcatacagt
    13201 ataagaccgt ctgtgtgctc gtgacaaagc ccacattcac taaggctaac ttactgttgc
    13261 atcgttacat actgccttaa agtcagcaaa aacgcaccgt taatattgac cagacaattg
    13321 caacttaata cttcattcat aaaagattac gccacggtac ataaagtaac cgtggcgtaa
    13381 tggctatcag accgctttaa tgtcgatggc ttcaccctgc atccgtttac gcagggtacg
    13441 tttcgtacgg tcgataacat cgcccgccaa ctgaccacag gcagcatcga tatcatcacc
    13501 acgggtttta cgcacaatag tggtgaaacc gtagctcatc agcacttttg agaaacggtc
    13561 gatacggctg ttcgagctgc gtccatacgg cgcgcccggg aaggggttcc acgggatcag
    13621 gttgatctta cacggcgtat ctttcagcag ttccgccagt tggtgcgcgt gttcagtgcc
    13681 gtcgttaacg tggtcaagca tcacatactc aatagtgaca cggccctgat tggcgttgga
    13741 tttctccaga taacggcgca ccgcagcaag gaacgtttcg atattgtact ttttgttgat
    13801 cggcacaatt tcgtcacgaa tttcgtcgtt cggcgcgtgc agggaaattg ccagtgcaac
    13861 gtcgatcata tcgcccagtt tatccagcgc cggaactaca ccggaagtag aaagcgtgac
    13921 gcgacgttta gacaggccaa aaccgaaatc atcaagcatg atttccatcg ccggaacgac
    13981 gttgttcagg ttgagcagcg gctcgcccat gcccatcatc actacgttag tgattgggcg
    14041 ctgaccggtg acttttgctg cgccgacgat tttcgccgca cgccacacct ggccaataat
    14101 ttccgatacc cgcaggttgc ggttaaagcc ctgctgggcg gtggaacaga atttacactc
    14161 cagcgcacac cccacctgcg aagagacgca gagcgtggca cggtcgtctt ccgggatata
    14221 caccgtttcg acgcgctgat cgccaacggc gatcgcccat ttaatggtgc cgtcggatga
    14281 acgctgttct tcaaccactt ccggtgcgcg gatttccgcc acctctttca gtttgccgcg
    14341 caacactttg ttgatgtcgg tcatctcatc aaagttgtcg cagcaatagt gatacatcca
    14401 cttcatcacc tgatcggcgc ggaagggttt ttcacctaaa tctttaaaaa actcccgcat
    14461 ctgctgacgg ttgagatcca gcaggttgat ttttccatct ttcgtggtga cgttttcagg
    14521 tgtgactaat tgttcagaca tatgctattc cggcctcgtt attacacgtt atggcccctg
    14581 gaggattgaa aaaagaaacg ccccggtgag cggctgctcg tccgggggcg ctgcattgta
    14641 caaattctgg cgcacggatg ccacgtttgc acgcggcatt tacgaaatta ttaacgggtg
    14701 cgcgggcaca cttcgccttc gccaaagaaa taagcgattt cgcgagcggc agattcgacg
    14761 gaatcagaac cgtgggtacc gttttcggtc aggctgtcag cgtaatcagc gcgcagagta
    14821 ccagccagtg cgtttgccgg attggtcgcg cccagcagat cgcggtgacg ctgaacggcg
    14881 ttttcacctt ccagcacgga aaccacgatc gggccagagg tcatgaattc aaccagacca
    14941 tcaaagaacg gttttccatc gtgttcagca taaaagccac gtgcctgttc aacggtcagg
    15001 tgcagcattt tggtgccgac aattttgaac cctgcagctt caaagcgcgc aaagatatta
    15061 ccaatgacgt tttttgctac cgcgttcggt ttgatgatgg aaaaagtacg ttcaatagcc
    15121 atttttacct ctgtaaattg ttctgttgtt gtctgtacca gcgtacgaga tggcgcggat
    15181 tataatgagc aacagggccg ttgactattg atgaaggtaa cattttttta aaataaaatg
    15241 agttttagca acaatcaggg acaaaacaga ctattgcatg acaaatttca ctgtcgcgat
    15301 ttgccccaca tcatccatca ccagcaattg ataatcacct ttatccgtca aatgcagggt
    15361 cacgttgcgc ccgcgttcag ttaacggttc gccattcaga aaccaccagc gttcacctgc
    15421 ccctccactt gattgcaacg gcaaagtcgc ttccgctgcg cccggtaaac gtttaataat
    15481 cgcgccatcg cgaacgcctg tcagctgcag agggagctgg gcatcgtgac cgtatgacgg
    15541 gcaaattgtc gaggccggtg gtaagcgcac agcgcgacgt tctgatgcgg gcagccaggg
    15601 ttccagcggt agcggccaga catttatcat ttcttgtcgc gcttgcgggc aatcagcggc
    15661 aacacgtttg ccattttcat ccagccagat ggggaaacga atgccattaa tgccttcctg
    15721 atccggcagt aatagagttg gcggctgact cccgtccagc agccaggttg ccaggcggcg
    15781 gcgacagtta ccgtcacctt ccggcaaaga ctgtccgccc ggccagcaga taacgccacg
    15841 agtgactgag ttcgggcgcg ggtcttccgg cagattcgca ctgcgcgaca gtaagatatt
    15901 attgacctga ttcaacaatg gtacggcact ggcaaagcca aactgaccaa caacgggcgt
    15961 gccgtccggt ctgccagtcc agatcccaat gacatagcga gcgttaaccc caatcgccca
    16021 ggcgtcacga tagccatagc tggtgcccgt tttccatgcc agtggggcga cgcgcggcaa
    16081 ggcactatcc ggcaagggtt tcgcttcatc agccataatc cggcgaatga tccacgccgc
    16141 ccccgacgac attaaaggcc gttcaagcag cggatcgtca ggctgtaagc gcaatttgcc
    16201 tgccttgccg tggcgagcaa acgcggtata cgctgccgcc atatcttcca gttttgcacc
    16261 agcgccgccg agaatgagtg aaagattcgg cgcagcaccg ttgggcaaat ataacggcaa
    16321 tccaacattg cgtaactttg ccgcaaaccg tttcggtcca taggcttcca gcacctgcac
    16381 agcaggtaag ttcagcgagc gcaccagcgc ctcgctcatg ctgatcgggc catgaaaacc
    16441 gctatcaaag ttacctggtc gataatcacc ggtgcgccgg gggacgtctt gcagcagtga
    16501 tgccgggtgg atcaagcctt catccagcgc cagaccataa acaaacggtt tgagcaccga
    16561 tcctggcgat cggatcgcat tgaccatatc aacatgacca aagcgtgaat catcgttgag
    16621 atcaaccgat cccacccagc cgcgaacacg catatcggta tgatcaacca cgatcatcgc
    16681 cagtgagctg cgcggtggca accgcccttt ccagttttgc gccagttctt ccagacgtcg
    16741 ttgaagaccg gcatccagcg tagtaacgat tttgtcgctt ttgcttttac cgagcatcat
    16801 gcgcgaaaac agcggtgcca gttgcggcat ttgtcggggt gccagccaga tgggttcttc
    16861 ccttgactct tttacctgct cacgggacca cacaccttgc acggccatcc gttcgagcac
    16921 tttattacgc gcggcttcgg cacgctccgg ccaacgatcc gggcgaagac ggctgggcgc
    16981 ttgcggcaaa accgccagca ttgccgcctc ggaatagctt aaattcgcag gcgactttcc
    17041 gagataagcc caacttgccg caccgatccc ctgcaacgta ccgccaaacg gagcgcggtt
    17101 aagatacaag gtcagaattt cacgcttaga cagatgccat tccagttgca acgcgcgcca
    17161 gagctggcga attttgccgc caaatgtttt ggggtgagga tcaagcagac gagcaacctg
    17221 catagtgagc gtgctgccac cggaaataac ccgtcccgaa gtgagatctt gccatgctgc
    17281 gcgcgccacc gagaatggat tcacccccgg atgcttccag aaccagcgat cttcataatt
    17341 gatcagcgct tcaaggtaac gtggagaaac atcttcgatt gttaccggat aacgccagat
    17401 gccgtcagca tcggcgaagc gccatagcgg cgtaccatcc tgcgccacga ccactcgtgc
    17461 gggattgact tcatgcagcg gtagaggcca gagtttatct gctccccacg ctgcaagaaa
    17521 aaggagaaat ggcgcagctg ccagcgttat ccagcagccg cgtttggtta acaagcgagg
    17581 catttacggt cggacaatca gcagatcttc agccgcgccg gtcgcccgcc attggggaac
    17641 atacattgat tccaccatcg gttgcggtac ctgatacgtt cccggcgtca ccgcccgcgc
    17701 cagatacacc agcgttaccg gttggtattc atcaacggca accgccgcca caaagcgatc
    17761 gtcacggaac tcaatgtgct taatactcgc ctgctgcatc tggttcagta agttttgcac
    17821 ttcgccacca ctttgctcca ggctggcgct accgttcgcc agattctgat tttccagttc
    17881 cagacccgca ggcagcagat ccacgactaa cgcatccggc acgctgttac tggcttttac
    17941 ctgcaaccag accagcacca gatcgccgct acgtaacgag tccagcgatt tgctcttacc
    18001 atcagtacca agaatatgac gctcgatttg cagcacattg ttcgccggta aaggtgcgga
    18061 ttgcggataa ccgctggcat ccacacgcag ccataacggc tgatcgccac tgtttctcac
    18121 ctgcaaggtg gcaagttgat cgctattcag attgctgttt agcgttttct cgcctgtcag
    18181 cggctcagct gagaaagagg tttgcgcctg ccatttaccc ggtaaatcct gaatcgtacg
    18241 ggcagccagg aacaacgcgt tactttcctg cgtcgatagc cagcgttcac caaatgcctg
    18301 ctgcgaaagc gtgttcagca aagtgttctg ctcatcgggt agcagtttat tttcttccag
    18361 caagtagagc attaacgcgt tgtcgcgcag tggactaccg taatcaccca gccatatccg
    18421 ctcatcacta tttctcggcg ttttcagcgc cagcacaatc gcttcttcac cacgcgttgc
    18481 atcacccatg gttttcagcg caacgccaag ttgcagcagc ggtaaaccag aagcggcatc
    18541 tgcacgatgc tcccagattt cacgcagcgc acccagtggt gctttttgct gacgggccag
    18601 caccagcgca gcgtaagact gaacggcgaa tttactggct ttgagattat ccgcgtacgg
    18661 gatcgacatc atgcccggat cttgtaaata gcgcagcaga cgctcattac cccggttaat
    18721 ggcgtctgtc ggcacgctgt aaccctgttc gcctgcgcgg accaggaaat ccatcacgta
    18781 agccgtcagc cagtactctt cgtcaccgtt tttatcccac agcgcaaagc cgccgttatc
    18841 acgttgcatt tgcagcaaac gggaaatgcc gatatcgacc gatgcacggc gtttctcatc
    18901 actgtcgcct ttgatgccca acgcctgcag ttgggctgcg ttggtataaa gtgacgggaa
    18961 caggccgctg gcggtttgct caagacagcc gtacggatac gcttttaact ctttgatata
    19021 acgtgcaatg ttcagcggtg gtttgccgct caacaacaat tgcccttcca gcgtaacagg
    19081 cgagaagttt tgcaatccat ccgccggaat cgcccatgtc tcaccaggct gtaacgccgt
    19141 accgtaatta accgtttgtg ccgggaacgc cggacggacg ccgattttcc actgcttatg
    19201 ctgatcggca acggtttcac ccggtaacgc taacccgcta atggtggcct gaatttctcc
    19261 atcgccataa cccggcaatg ctcgcaccgg gataaacagc gtagtacgca cacctggcgc
    19321 taattcaacg gctgcgggtg aatcgctgac cagttcaagc aaaccactgg cggtcagggc
    19381 aacgttcagt ttttgcggtt tatcggtaag attagtgata tccagcgtca gacgcgaggt
    19441 atcgccactc gccataaagc gcggcatgtt cagttcagca atcaccggtg cggcaactat
    19501 cactttgctt tcgttgctac cgaaatcatc tgccgtccag gcttgcgcca tgacgcgcaa
    19561 ttcaccgtta aaatcgccaa tcggcagtgt aaccgagcct tcgccctgtt cgttgagcgt
    19621 taccggcagc gcctgctgcg cgacaatatt gacgtgattg accggcggtt taccaccacg
    19681 tttcagctca tcaccatcgc caccgaaacg cagagctgcc agacgcccct gaccttcaat
    19741 aacctgaccg taaatatcgt aaatgtctgc gccatagcgt ttctgaccaa agaacgcctg
    19801 ccacggatct ggcgtgacgt agtcagtaat attcagcaca ccactatcaa cggcagacac
    19861 cagcacattc acctgtttag gcttctcgcc atttttagtg ctggctttaa ttttcacggt
    19921 taatggttga ttgggacgca tttttgctgg tgtttccagc gccagatcga ggcgacggtt
    19981 ttcatcgcca agcggcagat gcaacacacc aaccgcgcgt tttggcgtcg cggagcgaga
    20041 tttatcgcca ggacgtacca ccagcgtact taaatacaga tcatgacgat tccaggtttt
    20101 atcgaccgga atcgtcagat ccagcccttg agccggaaca tcaatctctt gccaccacag
    20161 cggcccttca ctggactcga ccatcgcata acctttaccc gccgttggcg cggcgatatg
    20221 caacttaatg gtgtcgccag ggcgataact ggctttatcc agtttcagcg tgacacggtc
    20281 gggtcgcact gcgccgctac cgtcgctgtt gtcctgccag ctatagccag cccagaaacg
    20341 aacactactg accgcttcat tcggcgcttt gacttccaga cgataagcgc cccactctac
    20401 cggaaaactg accttgccgg tttcgtccgc tttcagatcc agagtttgtt cattttcgat
    20461 cagatctttt tgatcaaact gtgactgcca gccttcatct tctgaccagt tccagtagta
    20521 atcgcggcgt tcgcgaatca ggcgcacctg caagcccgac acggcttttt tcacgccttg
    20581 cgcatcgcta taaacgatgt caaaagcggc gttactgcct tcatcaacaa tgggctgttt
    20641 caccgtgctg tcagtacgat aatcgtaaac cgatttcgag gcgaactgcg gacggatccc
    20701 cggcaatgca tcggcaggcc agatagcctg ctcagcgcgg cgcgtcaccg ggcgaccgcc
    20761 cgattccagc aaactaccct ggaaaataac ctgtaatggg gaatgcgttt ccttccactg
    20821 gctttctgta gaaacttcgc cgcgcccttt atcatccagc gtcaactgaa cttcatccag
    20881 cgtgcgggaa agattttcgg cagctatatc gccgaattca aaaccaggta aggctgacac
    20941 agcttcacgc agtgggcgca ggaaaagttg cccttgcaaa gtattaccat tagcaggtgc
    21001 accatacagg tagtacccca ccacggagaa tttcacttca tctttcggcg ttagcggggt
    21061 tttctcaccg gtcagattca gcgccatgcg ctctggcata aaatcttcga cgtggaaatc
    21121 ccacatccga tactggttat cgcccgtgtt agcgcgaata tgccacatac cggttgccgc
    21181 attgctatcg agtggccagg taaagtggta gaggccattc tccggctgac taacgacgct
    21241 cctgagtacc tgcccatcgg gtttaatcac gtctaacttg atgggttgat tgggcaacgc
    21301 tttaccgtct gcatcacgca gcaaaccatt gaggattacc gtttcacctg ggcgataaag
    21361 atcgcgtggg ccaaacatga aaaactgttt gctatagcct ggcgcgccag caatgttaaa
    21421 ttctgctaag tccagcgccg gaagttttaa atcgagtagc gttgtctgac cgtctttacg
    21481 cgccaacagt aatgccgcgt ttttatcatt ttccagctgc acatgcccct gagcgtcact
    21541 ggttgcctga gtcagagtct gccctttctc atttaataaa gagacttcaa ttccttgctg
    21601 ggccgcgccg ttttccagac tttgggtaaa gatatccaga cgattgtgat aacggtgagc
    21661 tgaaacgccg atatcactta acgtaaacag cgtcgcggga ttactgtaat cgtaacgtcc
    21721 agcctgattc atcacagcca gatacacgcc cgcctgctga agcggtttga tatcgcccag
    21781 cggcagcaat aatttttcac gggtgttacg cgcaggattg agatcaaacc gtccggtgta
    21841 gaccagatcc gccatctgca gcagtttgtc tgactgccag ttcgccagcg aattgcggta
    21901 ttcccattgg ctaatgaatg ctggcagaga ttctggctta acgcggaaga agttaacatc
    21961 gacattatta acgttgagcg ccattaccgg cagcccttca acgactttgc caggcagcag
    22021 cgaaccacgg ctggcaaaac cgacgctggg ttggatgtcg cgggtagtta tagttttttc
    22081 gtaatcttta ctgaaggttg cgttgttgag tgctttgacc tccttgccaa tagtaacgat
    22141 caaatcacgt ttcggttcga ggtggcgtaa acgtaactct ttaagattat ctgacagctc
    22201 ccaggcacca tccactttgc cgcttttttt atcgacgaca tgaataacgc gtgagaaatc
    22261 ctgatccggg tcgagaggga tggagaacgt cagcaccagc gtggctgcac catcaagttg
    22321 gacttcagag agatccagca atgtcagcac cttaccggca ctctgttggg ccagtttttg
    22381 tctctccggc acggagagtt ttgctgaact cgcgttttct gaagaggccg ctttagtaac
    22441 ttcagaaggc gcatcttttt tcaccgctgt tggcgcgtta tcgttgttgt cgcaccctgc
    22501 cagcgccagc attagcatgc aggcggctac gcgtaacttt ttcatttttc atccctgcca
    22561 caatggcccg ttagcaacgt cgaataatta ttatgcgtga gaatttacgt tatgtaattc
    22621 agtatcacgc ccatacaatc cgaattttag tgagaattgt ggctcagaca gccaaatcag
    22681 cctctttcgc ttgtcgcttt tcactaaaca tccgacaatt tagcctaacc ccggcaaaaa
    22741 tggagatgcc tatgtccacg acatggtttg taggagccga ctggctcgcc gaacatattg
    22801 atgacccgga aattcagatt attgatgccc gcatggcgtc gcctggacag gaagatcgta
    22861 acgttgctca ggagtatctg aatggacata ttcccggcgc agtgtttttt gatatcgaag
    22921 cgctttctga tcacacttcc ccgcttccgc acatgctgcc gcgcccggaa acgttcgccg
    22981 tggcgatgcg tgaattaggc gttaaccagg ataagcacct gattgtctat gacgaaggta
    23041 atcttttctc agccccacga gcatggtgga tgctgcgcac ctttggtgta gagaaagtgt
    23101 cgattctggg gggtggactt gcaggctggc agcgcgatga tctgctgtta gaagaaggtg
    23161 cagtagagct gccggaagga gagtttaacg ccgcgtttaa tcctgaagcc gtggtgaaag
    23221 taaccgatgt attattggca agccatgaaa atacggcgca aattattgat gcccgcccgg
    23281 ctgcacgttt taacgcagaa gttgatgaac ctcgcccagg tttacgtcgc ggacatattc
    23341 ccggagcact gaatgttccg tggacggaac tggtgcgcga aggcgaacta aaaacgaccg
    23401 atgaactgga tgcgatattt tttggtcgcg gcgtcagcta cgacaaacca attatcgtca
    23461 gctgcggctc tggtgtaacg gcagccgtgg ttttgttagc actcgcgacg ctggatgtgc
    23521 caaacgtgaa actgtacgac ggcgcatgga gtgaatgggg cgcgcgggca gatttaccgg
    23581 ttgagccagt gaaataagta ttttacaggc aataaaaaac cgccgaattt ggcggttttt
    23641 tattgctggt ctggttcgcg gcctttccag caggttgact tgtgttacat gagcaacgca
    23701 ggtgcttcac agcaaaacaa tactcaccag taactctctt tttgtcaagc aaaagagagt
    23761 aattattgtt tatttagcgt attatcgaca ccggcccttt ccgccgtgtt cggtaataaa
    23821 ttaacctggc ttattagtcc gaattcagac aaatataaac aaatcctgct caaaattaaa
    23881 aatactaacc ggtaaaagat attacttaaa catgtaaatt cactttcctt taaaaaacaa
    23941 aaaaccgcca aaatcaggcg gttatttgtt gctggtccgg ttcgcggcct ttccagcagg
    24001 ttgtattacc gtagtaatgc aagcgcgtct cagcggagac aatactcgcc agtaactctc
    24061 tttttgtcaa gcaaaagaga gttattattg ttctgttagt gtattatcca ctgcggccct
    24121 ttccgccgtc tcgcaagcgg gcgctggctt aaggaaagga tgttccgcga ccgttagagc
    24181 aagcgcgtct cagcgcttgc tatcgcggca atatcgccag tggtgctgtc gtgatgcggt
    24241 cttcgcatgg accgcacaat gaagatacgg tgcttctgta tcgtacttat tgtttctggt
    24301 gcgctgtttt cccaagtaaa taataaccgg agtctctccg gcgacaattt actggtggtt
    24361 aacaaccttc agagcagcaa gtaagcccga atgccgccct ttagggcggc atattttaga
    24421 ttatccggtt ctgtttaaag tcacgcaaaa aaccacccca gcgacgttca tagaatggcg
    24481 caatatgttc ggtaataaag tggctaattc ctttttcccc ttttttcacc tgacaaatat
    24541 cgattggttc atcgccaggt aatgtatcgg tcgctacact tcccgtcgcc tgaataattt
    24601 cttcgatatc accatcggct tcaatgccaa taagtaaatt aggctgtgcc tcttcgttct
    24661 ctttaattga acaaataaaa gcacgcttca ccggcttaat ggttttaaat aaggtggtga
    24721 gtgaatcaat catttgtgct ggcggctctg cgacttccga taatatcagc gattcaccgc
    24781 cttccaggat ttcctggctg ctcagcggat ttccctctcc accaatcaac aaactgattt
    24841 cacgcggcat aaattcttta ccggtcggca gtttggcatt aaggaagagc gtttcgccaa
    24901 gggtcatctc aaacaatgtg cgaacgggca ttacgacaaa tgcctgttcg tcttcaaccg
    24961 cctgttgaag cgcttctaac gaggtgaaaa aaggaatgac gctggtgccg tcttcttttt
    25021 cccagtgctg taaatcaagc gcactatctt caaccacagc ctcgccctgc gccgccgtac
    25081 caggcaccca gacggtggat tccagtagag tacggaaaaa ggccgggcgg tgcgccggtt
    25141 cggttgctgc tttttccagc aggtcttcaa gttcgttttt tgtttcggac ataagaacca
    25201 caattcattc aacgttcggg gcgcaaatgc ctgatgcgct acgcttatca ggcctacaag
    25261 ggattcgcaa tttgttgaat ttgcagaatt tgtaggccgg ataaggcatt tacgccgcat
    25321 ccggcatatt agtttacgcc gttaacagat tagcaatcgt gcgcacacca agtcccgtag
    25381 cacccgcaga ccactgttca accggcgctt tacggtaagt cgccgagcag tcgatatgca
    25441 gccagccttg ctgatagttc tcaacaaagt gcgacaggaa gcccgccgcc gtgctcgcgc
    25501 ctgccggata cgccgcgctt ccggtattgt tcagttcggc aaagttagac ggcagctggc
    25561 tgcggtggaa ttccgccagc ggcagacgcc agaatggttc gttctcttgt gaggcactcg
    25621 ccagcaaacg accggcaagc gcatcgtcaa aactgaacag cgcgtgataa tcattaccca
    25681 gcgcagtttt cgccgccccg gtgagggtcg ccgcatcaat gatcagttcc ggtttctgcg
    25741 cactggcatc aatcagacca tcggcaagca ccagacgccc ttcggcatca gtgttcatca
    25801 cttcaacttt tttaccgttg cgataggtga tgatatcgcc cagcttgaac gcattgccgc
    25861 taatcaggtt atccgcacag cagaggaaca gcttcacgcg cttgttcagt ccgcgcgtaa
    25921 tggcaaatgc cagcgccccg gtaaccgttg ccgcgccgcc catgtccgac ttcatcgagt
    25981 ccataaacgc agtctgtttg atgctgtagc cgccggagtc aaaagtgata cctttaccta
    26041 ccaggcacgc gtacactggc gcttctttat cgccagttgg gttgtaatcc agcgccagca
    26101 ataccggaga acgttctgaa ccgcgtccga cggtgtgcag ccccatataa ccttgctcac
    26161 gcagatcttc gcctttggtg atccgataag tcacacgatc gcccgcgacg ttgctgatca
    26221 gatcaacagc acgctgtgcc agttgcgatg gtcccaattc ttctgccggt gcgttgatgg
    26281 tgtcacgcac ccagtcgatg atcatcaggc ggttatccag ttcctggcgc tgggcatcgt
    26341 ccagatccgg ccactccact ttacgcgtgc ctttcggggc tttgtaacct tgccagaatg
    26401 cccagcagcg atccgcatcc cagccttcac cgcttaactg aacatgcttg atgcccagac
    26461 cgtcaatctt gcgcgccgca cgctggatca gccccagatc gtctgccccg ttcaaatgca
    26521 gggtaatgcc gtcattatta atgctgtaag ttgctttttc tccccagcgc gcgtcggcag
    26581 gttgggtaga gagggtaatc ttcatcgctt ctgtcatttt agttatcctt ctttgtaaaa
    26641 gggccgccag ttggcagccc caggattttt ccgctaaatg attcgcgttg caggaaggcg
    26701 gcaagtgagt gaagccccag gagcatagat aactatgtga ctggggtgaa cgagcgcagc
    26761 caacgcatct gcggcgtgaa gcatgacgcg gaaattattc ggcctcgtcc agccagacta
    26821 acaaaatcgc ttcgaggatt ttctcgttgg atgcctgcgg gtcgtcgtcg aaatcttcca
    26881 gatcgcaaat ccactgatgc atatcggtga atcgaaccgt tttcggatca agatcgggat
    26941 acgcatcgta cagtgcttcg ccaatttcgc ggctatcggt ccatttaagt cccatactaa
    27001 cctctgttaa tgctcacgcg catggttgat agtgtaacgc gggatttcga ccactaaatc
    27061 ttcgtcggtg acgcgcgcct ggcagcttaa acggctttcc ggctccagtc cccaggcttt
    27121 gtccagcatg tcgtcttcct gctctgagct ttccggcagc gagtcaaaac cttcacgaac
    27181 gatgcagtgg caggtggtgc aagcacagga tttttcacag gcgtgttcaa tctcgatacc
    27241 gttacgcaga gctgcgtcga gaatggtttc accgctatta gcttccagaa cagcgccatc
    27301 agggcagaga tcctgatgag gcaaaataac aatctttggc atattaaacc tcgtccacgg
    27361 aatggccttt cagcgcacga cgaaccgact ggtccatgcg gcgagcggcg aaatcctggg
    27421 tttgtttgtc tacgttttta atcgcttgtt cgatggcgtc aacgtcatcg ccctgcgcca
    27481 cttcactcag gtgagcggca gcatcgtcaa tgacctgacg ttctgcggcg cttaacagcg
    27541 cggcatcagc agccagcgcg ccgtgcagac tttccagcac acgcgccgct tctacttttt
    27601 gttctgccag cattcgggct tttacgtcct gctcggcata gctcattgag tctttgatca
    27661 tcgaagcgat ttcgctatcg gtcagaccgt aagacggttt gacctgaata gacgcctcaa
    27721 cgccggtgga tttctccatc gccgtcacgc tcaaaagacc gtcggcatcg acctggaacg
    27781 tcacgcgaat atgcgcacca ccagccggta gcgccggaat accacgcagc gcaaaacgcg
    27841 ccagtgagcg gcagtcctgc accagttcgc gctcaccctg cattacatgg atagacatcg
    27901 ccgtctgacc atctttaaag gtggtgaaat cctgagcgcg ggccaccgga atagtggtat
    27961 tacgcggaat cactttctcc accaggccgc ccatcgtttc gaggcccagc gacagtggga
    28021 tcacatcaag cagcagcatt tcgctgtctg gcttgttacc caccagaata tccgcctgaa
    28081 tcgccgcgcc aatagcgacg actttatccg ggtcgatgga agtcagaggt ggacgaccga
    28141 aaaattcgcc tacccgttca cgcaccagcg gcacgcgagt agaaccgccc accatcacca
    28201 cttccagcac ttcatcagct tctacacccg cgtctttcag cgcgcgacga caagccagta
    28261 aggttcgttt taccagtggc gcgatcagtt cattgaattg ttcacggctg atttcgccct
    28321 gccagcccgc aacgttaacg gtcacggagt ccgcatcgct cagcgcgatt ttggctgcaa
    28381 tggcggcatc cagcagttca cgctgaacgc ggttatcgct acgatcagga atgcccgcct
    28441 gctcgcgaat gtaatccgcc agcagatggt cgaaatcatc gccgccgagc gcggaatcac
    28501 cgccggttgc cagcacttca aacacgccgc gacttaagcg cagaatggaa atatcaaacg
    28561 tcccgccacc gaggtcataa acagcgatca cgccttcctg accggaatcc agcccgtagg
    28621 cgatagccgc agcggtcggt tcgttaagta agcgcagaac atgcaacccc gccagacgcg
    28681 ccgcgtcttt ggtgccctga cgctgggcat cgtcaaagta cgccggaacg gtgataacta
    28741 caccatccag ctcgcctgcc agggcttcag ttgcccgcgc cgccagtgct ttgaggatgt
    28801 ccgcagaaac gcgcaccggg ttcagcagcc ccgccgccgt ttcaatcatc ggcaggccgt
    28861 tttcgctggc ctggaattga taaggcagat gcggatagcg ttgctggata tcagccagcg
    28921 agcgccccat caggcgttta acagaactaa tggtgttggc ggtatcaagc gctgcgttag
    28981 tacgcgcgtc ataacccacc gaatgccctt gctgttgata gtgaacaaca gatggcagca
    29041 ggtgacggcc ttcatggtcg gctaacgttt cggcctgacc gctgcgcact gtcgccacca
    29101 gcgagttggt tgtgcccagg tcaataccag ccgccagacg acgctgatgc ggcgcagcac
    29161 tcaaaccagg ttcactaatt tgtaataagg ccatgtttag cttccagaaa ttaaaaatcg
    29221 agcagttttt cttcgagttg ttcggcactg ctccgcagtt tatcgagaaa acgcagctta
    29281 cgcacggtat ccgccgccgc gtcccacgcc tcgttgtcta actgttcaac catcaactga
    29341 tggcgggtat caaacatctt tttcacacgt ttgataaagc tttccagccg cgcttcatct
    29401 ttcgcctgtt cgatctcgtc cagctcttcg cgcagctcca actgttccat caggaacgcg
    29461 gtgtcgcgca cagtatgctg ctcgctggcg agatcaaaac cgtgtaaaga aagcaaatat
    29521 tccgcgcgca ttaacggatg acgcagcgtt tgccaggcct ggttaatggt tgcagattgc
    29581 tgtacggcgg cgagttgttc cgcctggctt ccgctggcga atttatcagg atgatactga
    29641 cgttgtagat cctgaaaacg caggctcagc gcctgggtat cgagttgata acgggcaggc
    29701 aagccaaaga gggtgaagta atccataaca ttctcagggc tgcgatccgc cgcgataacg
    29761 gcgaatcagg taaaacaaac cccacgcgca ggcgaccacg gtggggttat cggtatgcgc
    29821 atcaaacgtg gaagctttcg ccgcaaccac actcatcttt aacgttcggg ttggtgaatt
    29881 taaacccttc attcaggcct tcttttacga agtccagctg cgtaccgtcc agaaattgca
    29941 ggcttttgcc atcgaccacg actttcacgc ctttgtcttc aaacacgatg tcttccggcg
    30001 tcggttcgtc aacaaattcc agtacataag ccatacctga acatccggag gttctcacgc
    30061 ccagacgcag gccaaaccct ttgccgcggt tagccagaaa ggtatttact cgcgctgctg
    30121 cactgtcgct cagtgtaatc gacataacca aacctcaact cttattttgc ttcacgtttg
    30181 cttttatagt ccgcaatggc ggctttgatc gcgtcttctg ccagaataga acagtgaatt
    30241 ttcaccggcg gcagttcaag ttcttcagca atatcggtgt ttttgatcgc ctgcgcttcg
    30301 tcgagagact tccctttcac ccattcggtg accagggagc tggaagcgat agcagaaccg
    30361 cagccgtaag ttttaaaacg cgcgtcttca atgatacctt catcgttgac tttaatctgc
    30421 aacttcatca cgtcgccaca ggccggtgcc cccaccatgc cgctgccgac gttctcgtcg
    30481 ttgttgtcaa aggaacccac gttacgcgga ttctcgtaat ggtcgataac tttttcgctg
    30541 taagccatta taaattctcc tgattccgat accgattaat gatgagccca ttcgatgctg
    30601 ttcagatcca cgccctgctt gtacatttcc cacagcggag aaaggtcacg cagacgaccg
    30661 atggatttac gaactaactc gatggtgtag tcgatctctt cttcagtagt aaaacgacct
    30721 aaagagaaac ggatagagct atgtgccagc tcgtcgttca gccccagcgc gcgcagcacg
    30781 taggacggtt cgaggcttgc tgacgtacag gcggaacctg aagaaactgc gaggtctttc
    30841 agcgccataa tcagcgactc accttcaacg tagttgaagc tgacgttgag aatgttcggc
    30901 gcaccgtgtt ccaggtcacc gttcaggtaa acttcttcga tatctttgat gccgttccac
    30961 agacggttac gcaggccgcg cagacgttcc atctcggtcg ccatctcttc ttttgcgatg
    31021 cgataggcct cgcccattcc gacgatctgg tgaacaggca gagtgccgga acgcataccg
    31081 cgctcgtgac cgccgccgtg catttgcgct tcgatgcgta cgcgcggttt acgacgtaca
    31141 tacagcgcac cgataccttt cgggccatag attttgtgac cggagaaaga catcaggtca
    31201 actttcaact ggctcaggtc gataggcagt ttacccacgc tctgggttgc atcaacgtga
    31261 tagataatgc cacgagcacg gcacatttcg ccgatagccg cgatatcctg caccacgccg
    31321 atttcgttat ttacgtgcat gatggaaaca aggatggtgt cgtcacgcat cgctgcttca
    31381 agttctttca ggtcgataat gccgttacgc tgcggtgcca ggtaggtgac ttcaaaacct
    31441 tcgcgctcca gctgacggca ggtatccagt accgctttgt gttcggtttt gctggtgatg
    31501 atgtgcttgc cttttttctg ataaaagttg gctgcacctt tgatcgccag gttgtcagat
    31561 tcggttgcac cagaggtaaa gacgatttca cgcggatcag cgccgaccag atcggcaatc
    31621 tgattacggg cgatatctac cgcttcttca gcctgccagc cgaaacggtg agaacgagag
    31681 gccgggttac caaaggttcc gtccatcgtc atgaactgca tcattttctc ggcaacacgc
    31741 gggtccaccg gcgtggttgc ggagtagtcg agataaatcg gtaatttcat tgctctataa
    31801 actccgtaca tcactcaatg caaggaatca ggctaccggc tggatgtacg accgtgttta
    31861 cggagtattt agcactccgg cctgattctg aattcttttt attaagcgcg taacttaacg
    31921 tcgatcgcgt cttgtgtgcg ggtgcgtggc gcgtcgtgag tatgctgacg accagacaca
    31981 tccagcactt cctggttatt aaccagttcg cctaaagtaa tgttgttgag aaaaccggtg
    32041 agacggtcgc tcaaatcacg ccacagcgcg tgggtcaggc atttatcgcc gccctggcag
    32101 ccgcctttac cctgacaacg ggtggcatct acagattcgt caacggcgct aatcacttcg
    32161 ccaacggcga tgctgctggc atctttgcct aacagataac caccgcctgg tccacgtacg
    32221 ctggaaacca gaccattttt acgcagacgg gaaaacagtt gttccagata agaaagggaa
    32281 attccctgac gttcggaaat atcagccaac ggtaccgggc ccgcttcaga gttgagcgca
    32341 acgtcaagca ttgcggtcac ggcatagcgc cctttagatg tcagtctcat gtcttacttc
    32401 acctcaaact cgcccctgcc cggggttttt tattgtaaaa gtgggggtat tgcatagcag
    32461 ggtcaagtct gacattcccg agtaaattgg tcaactattt acttgactga tttactcggg
    32521 tatttaacct tcagtgccat ttttttatcg tggcgtttgt acctgttgtc ggatgcggtg
    32581 tgaacacctt atccgaccta cggttctgta cattgtaggc ctgataagac gcgttaagcg
    32641 tcgcatcagg caactactgt tattcggcct tgttaccttt attctgctgc tcaatagaag
    32701 ccagaatccc gcgcaggata ttcaattcct ggctttccgg gcgcgcacgg gtaaacagac
    32761 ggcgcaattt attcatcacc tgccccggat ggttttcacg gataaaaccg gttgccagca
    32821 gcgtttgttc cagatgacca taaaaacgct ccagatcatc gaccagcgga tacggcgtct
    32881 cttcatgctc gacctgctcg ccgttttcct gagttgccag ccaggccatg cgcacttcat
    32941 aggcaataac ctgaaccgcc atcgccaggt tcagcgagct gtattccggg ttagccgcaa
    33001 tcgcgacatg ataatggcat ttctgcaact cttcattggt caagccgacg cgctcacgac
    33061 caaacaccag cgccaccggg gtatttgccg cttcagcgac gcttttcaga ccgcattcgc
    33121 gcgggtcgag catcggccac ggcagcgtgc gggagcgtgc gctggtgccc accaccaggc
    33181 tacaaccggc taacgcttcg tcgagcgtat cgacgatgtg agcattacca atcacatcgc
    33241 tggcccctgc tgccagggca atcgcctggg agtcgggttt caccagtgga ttaaccagcc
    33301 acagattggt taatcccatt gttttcatgg cacgggcaac agaacccata ttgccggtgt
    33361 gtgacgtctc caccagcaca attcgaatat tttgcagcat tgtctttctt catctaaaga
    33421 ttattcacgc atcttatcat aaaacgaaga cagatgccga tctcgctgct atactctgcg
    33481 ccgttttccc gttctttaac atccagtgag agagaccgat gcatccgatg ctgaacatcg
    33541 ccgtgcgcgc agcgcgcaag gcgggtaatt taattgccaa aaactatgaa accccggacg
    33601 ctgtagaagc gagccagaaa ggcagtaacg atttcgtgac caacgtagat aaagctgccg
    33661 aagcggtgat tatcgacacg attcgtaaat cttacccaca gcacaccatc atcaccgaag
    33721 aaagcggtga acttgaaggt actgatcagg atgttcaatg ggttatcgat ccactggatg
    33781 gtactaccaa ctttatcaaa cgtctgccgc acttcgcggt atctatcgcc gttcgtatca
    33841 aaggccgcac cgaagttgct gtggtatacg atcctatgcg taacgaactg ttcaccgcca
    33901 ctcgcggtca gggcgcacag ctgaacggct accgtctgcg cggtagcacc gctcgcgatc
    33961 tcgacggtac cattctggcg accggcttcc cgttcaaagc aaaacagtac gccactacct
    34021 acatcaacat cgtcggcaaa ctgttcaacg aatgtgcaga cttccgtcgt accggttctg
    34081 cggcgctgga tctggcttac gtcgctgcgg gtcgtgttga cggtttcttt gaaatcggtc
    34141 tgcgtccgtg ggatttcgcg gcaggcgagc tgctggttcg tgaagcgggc ggcatcgtca
    34201 gcgacttcac cggtggtcat aactatatgc tgaccggtaa catcgttgct ggtaacccac
    34261 gtgttgtgaa agccatgctg gcgaacatgc gtgacgagtt aagcgacgct ctgaagcgtt
    34321 aatgactcag gcgggtgatg tcactcaccc gccctcgcct ttcaggcgct attctgaaat
    34381 acttcctcac cgctttactt tctttcgtca ctctcccacc atctttcccc gattaatgga
    34441 tgataaagaa gtcgcaagga aaggttatgg cactgccagt gaacaaacgc gttcccaaaa
    34501 ttctgtttat tctctttgtt gttgccttct gcgtttattt agtgccgcgc gttgccatca
    34561 acttcttcta ttatcctgac gataaaattt acggtcccga cccctggtcg gcggaatccg
    34621 tcgaatttac ggctaaggac ggtactcgtc tgcaaggctg gtttattcct tcttcgacgg
    34681 gccctgctga caacgccatc gcaaccatca ttcatgctca cggcaatgcc ggaaatatgt
    34741 ccgcccactg gccgctggtc agttggttac ccgagcgtaa tttcaacgtt tttatgtttg
    34801 attatcgcgg gtttggtaaa tcaaaaggca cgccgtccca ggccggattg ctggacgata
    34861 cgcaaagcgc catcaatgtg gtgcgccatc gcagtgatgt aaacccacaa cggctggtgc
    34921 tgttcgggca gagtattggc ggggcgaata ttcttgctgt tattggtcag ggtgatcgtg
    34981 aaggcatacg tgcggtgatc ctcgactcca catttgcctc ttatgcaacc atcgccaacc
    35041 agatgatccc cggcagtggc tacttacttg atgagagtta cagcggcgaa aattatatcg
    35101 ccagcgtcag cccgatcccg cttttactca ttcacggtaa agctgatcac gttatcccat
    35161 ggcagcacag cgaaaagttg tatagcctgg caaaagagcc aaaacggttg atcctaatcc
    35221 cggatggcga acacattgat gctttttccg atcgccacgg cgatgtttat cgcgaacaga
    35281 tggtggactt tatccttagc gcgttgaatc cgcagaacta acccatgatc gctagcacga
    35341 taatcattca caaaaccacc ttaagacatg ctaatccact ggtcagaaca gtttaagatg
    35401 agaaaaattc tgtgacgctt gccaacattt ctgatgatta gcattccctt cgtcatttcc
    35461 ctgagcaaac tttagctatt cttatcaatt atgcttatgg gagatctaca gatgatgcct
    35521 acgcttgctc caccatctgt cctttcggct ccccagcgcc gctgccagat cttgctgacg
    35581 ctctttcagc cggggttgac cgccaccacg gcaactttca gcgagcttaa tggtgtggat
    35641 gatgatattg ccagtcttga tatcagcgaa acaggacagg agatcctgcg ctatcatcaa
    35701 ctcacactga cggctggtta tgacggtagc taccgggttg aaggtacagt gcttaaccaa
    35761 cgtttgtgtt tatttcactg gctacgacgt ggtttccgtc tgtgtccatc atttattacc
    35821 agccatttca cccccgccct gaagagtgaa ctgaagcggc gcggaattgc gcgtaacttt
    35881 tatgacgata ccaatctaca agcgttagtg aatctctgct cccgacggct gcaaaaacgc
    35941 tttgaaacgc gcgatattca tttcctgtgt ctgtatctgc aatattgttt gctgcaacac
    36001 cacgctggaa ttacgcctca gtttaatccg ctccaacgtc gctgggcgga atcctgcctt
    36061 gaatttcagg tagcgcagga aattggacgc cactggcagc gtcgggcgct ccagcctgta
    36121 ccacctgatg agccactgtt tatggcacta cttttttcca tgttgcgggt tcccgatcca
    36181 ctgcgggatg cgcatcagcg agacagacaa ttgcgtcagt ctatcaaacg tctggtaaac
    36241 cattttcgtg agctgggaaa tgttcgtttt tatgatgaac aggggttatg tgatcagctt
    36301 tatacccacc tcgcccaggc gttaaatcgc agtttgtttg ccatcggtat tgataatacc
    36361 ctgccggaag agttcgccag actgtaccca cgcctggtgc gcaccacccg cgcggcgctg
    36421 gccggatttg aaagtgaata cggcgtccat ctttctgatg aggaaagtgg tctggtcgcg
    36481 gtgattttcg gtgcctggct aatgcaggaa aacgatctgc atgaaaaaca gattattctt
    36541 cttaccggga atgatagcga gcgagaagcg cagattgagc agcagttacg cgaactaacg
    36601 ttactgccgc tcaacattaa gcatatgtcg gtaaaggcat ttttgcagac aggcgctccg
    36661 cgtggcgcgg cactgattat tgcgccttat accatgccat taccgctctt ttcaccaccg
    36721 ctgatctata cggacctgac gctgacaaca catcaacagg agcagatccg caaaatgctc
    36781 gaatcagcat gagggaacaa ctttcgggcg caaaaacatt gccggaagcg ccaccagcgc
    36841 cattacccag aacacgccgt ggcccagata ttgatacagg aaaccggcga aaacggtcat
    36901 gatagcgata ctgccgccca tcgcgacggc agagtaaacc gcctgtaaac ggatgacttc
    36961 gctaccctgg cgagcagcaa tataacgcat ggcggccagg tggcagaccg tgaaggtgcc
    37021 gcaatgcaga atttgcacca ctatcaacca cggcaacgcc gtagttgctc ccataatgcc
    37081 ccagcgcact acgccgcaaa tcgccgagat caacagcata tcgcgtgcac tacaacggcg
    37141 gaaaagttta ttactcagcg caaagataat gacttccgcc accacgccca gcgaccacaa
    37201 ataccccacc gccgaggccg agtagccagc tgcctgccag taaatggcgc taaaaccgta
    37261 ataggccgca tgtgccccct gcaataaaca aacgcaggcc agaaagcgcc agttctggcg
    37321 aaccagcgcc aaccacgctg accaaccggt gctctcctgc tggcggcttt ccccttgtgg
    37381 ctgaatcgtc ggacggatga gaaagccgag cagcatggat gccacgccca acgtcaacag
    37441 cgcgaggatc acccgataat caaacatagt gaccagtttg cccgtcagcg ccgagccaat
    37501 gacaaacgcc accgagcccc acagtcgcac tttgccgtaa tcaagcggga actgcttttg
    37561 ccacgtattc gccagtgcat cggtcaacgg taccagcggt gagaaaaaga ggttaaagcc
    37621 aatcatcacc agcatcagcc acgctacgtg cgcccccgcc cagaaggcga cagcaaagag
    37681 aagtgtcagc agtgccagca cgcgcaaggc ggaaatcagg cgggaaggat cgctgacgcg
    37741 gggcgcgatg agcaaactcc cgagaaaacg ggcaaccaga cctgccccca ataacaggcc
    37801 gatggtttct ggcgttaaac caatcccttt aagccagacg ctccagaaag gtagaaaaat
    37861 gccgtaacta aaaaagtatg tgaaatagcc gagcgccaac cagcgcgtgg attgcaaaac
    37921 catgagtccc tcccgtcagg aggcgttagt ctggggttaa tcgaatgttg gtgcaagttg
    37981 aaaaataaga atattcgagc ctgcttcccg atgtctgtaa cgccagttca caagcactaa
    38041 aaaactgcga tccgcgacgc atgttgcaaa aataatgcgt aattatgccg ttacgtttgc
    38101 caaacgttcc tgcacaatgg cgatgaaatc gcgtaatgcc ggcttcatct cccctttctt
    38161 ccacgccatc agtaaagcaa tggaaggcac atttccggca atgggacgaa aaacaacctg
    38221 tccggtatta aaattattca tataaccggg tatcaaagtg acgcccagcc ccatgcccac
    38281 cagattcatg gtcaccagaa tattcgttgc cacctggacg atatttggct ggctgttttc
    38341 ttgcgcaaac cacgctttaa cgatcggcgc aagcgaaccg gaatacgccg gatcggtact
    38401 gacgaaattc acgccatcca gttgcgcggc ggtgatctct ttttcatgtg ctaaagggtg
    38461 atcaaccggt aacacaacca ctaatggttc gtcaaaaagc tccagataat caatctccgg
    38521 gctataaaca ggatggcgca ttaagccgac gtcgagttca ccacggcgaa ttttttcctc
    38581 ctgttgcgtg gtgattaaac tcaccagctc aatcaaggtg tctggctgtc tgagacgaaa
    38641 catcggtaat acttttggca gtaaattcac ttccgccgat ggcacaaagc caatggttaa
    38701 ttgtctgtct tcctgaacaa ttttccgcgc ccgtaatttg gcattttccg cttgttcgag
    38761 gattgccagc gcgtcctgga gaaaacattc tcccgccgcc gtcaacgcga ctttgcgctt
    38821 atccctcacc agtaacggaa caccgacaca gttttcaaga tcgcggatct ggctgcttaa
    38881 cgaaggctgt gaggtatgca gtttttccgc cgcacgggta aagttcagtg cctgcgccac
    38941 tgcgacgaaa tagcgtaaat gccgtagttc catcaccttc cccttgttat cgaaaaaacg
    39001 tctcagctgg tagaaattaa ctatttcaca tattagcaac caaccagcaa catccttatg
    39061 gcacaaaaat agaaggtcaa tacatctgat ctttcaggat taaaaaatat gaccacaccc
    39121 tcagatttga acatttacca actgattgat acccaaaatg gtcgggtcac tccgcgtatt
    39181 tataccgacc cagatattta ccaactggag cttgaacgta ttttcggccg ttgctggcta
    39241 tttctcgccc acgaaagcca gatcccaaaa cctggtgatt tctttaacac ctacatgggg
    39301 gaagatgcgg ttgtcgtagt gcgtcagaaa gacggcagca tcaaggcgtt tctcaaccaa
    39361 tgccgccacc gggccatgcg tgtgagttat gcagattgcg gcaacactcg cgcctttacc
    39421 tgcccgtatc acggctggtc ttatggcatt aacggcgagt tgatcgatgt accgctggaa
    39481 cctcgcgcct acccacaagg gttgtgtaaa tcccactggg gactaaacga agttccttgt
    39541 gtggagagtt ataaagggct gatttttggc aactgggata ccagcgcacc gggcctgcgt
    39601 gattacctgg gtgacattgc ctggtatctg gatggcatgc tggatcgtcg cgaaggcggc
    39661 accgaaattg tcggcggcgt acaaaagtgg gtgatcaact gtaactggaa attcccggca
    39721 gagcagttcg ccagtgacca gtatcatgct ctgttcagcc atgcttctgc cgttcaggta
    39781 ttaggggcga aagatgatgg cagcgataag cgcctcggtg atggacaaac cgcccgcccg
    39841 gtgtgggaaa ccgccaaaga tgcgctgcaa tttggtcagg acggtcacgg tagcggtttc
    39901 ttctttactg aaaaaccgga tgctaatgtc tgggtcgatg gcgcagtttc aagctattac
    39961 cgcgaaacct atgccgaagc agaacaacgt ttaggtgaag ttcgcgccct gcgcctggcg
    40021 ggtcataaca atattttccc cacgctttca tggctaaacg gcactgccac actccgcgtc
    40081 tggcatccgc gcggccctga tcaagttgaa gtgtgggcgt tctgtattac tgacaaagcc
    40141 gcctccgatg aagttaaagc cgcttttgaa aacagcgcca ctcgtgcttt tggtcctgct
    40201 ggttttctcg agcaggatga ctcggagaac tggtgtgaaa tccagaaatt gcttaaaggc
    40261 caccgcgccc gcaacagcaa actgtgtctg gaaatggggc ttggtcagga aaagcgccgc
    40321 gacgacggca ttcctggcat tactaactat atcttttcag aaactgccgc tcgtggaatg
    40381 taccaacgct gggccgatct tctgagtagc gaaagctggc aggaagtgct cgataaaacc
    40441 gccgcttacc agcaggaggt gatgaaatga gtgcgcaagt ttcactagag ttacatcacc
    40501 gcattagcca gtttctcttt cacgaagcca gcttactgga cgactggaaa tttcgtgact
    40561 ggctggcgca gctcgacgaa gagattcgtt acaccatgcg caccacagtt aacgcgcaaa
    40621 cacgcgaccg ccgcaaaggc gtccagccac cgacaacctg gatttttaat gacaccaaag
    40681 accagctgga gcggcgaatc gcccgtctgg aaacgggcat ggcctgggca gaagagccgc
    40741 cgtcacgcac ccgtcactta atcagcaact gccagataag cgaaaccgac atcccaaacg
    40801 tatttgctgt gcgggtaaat tatctgcttt atcgggcaca aaaagagcgc gatgaaacat
    40861 tctatgttgg aacgcgtttc gacaaagttc gccgtctgga agatgacaac tggcgcttgc
    40921 tggaacggga tatcgtcctg gatcaagcgg taatcacttc ccataacctg agtgtactgt
    40981 tctgatgaat cgaatttatg cgtgtcccgt tgcggatgtg ccggagggtg aggctctccg
    41041 gatcgatacc tcgcccgtca tcgccctgtt caacgttggc ggcgagtttt atgccattaa
    41101 cgatcgttgc agccatggta atgcgtcgat gtcagaaggg tatctggaag atgacgccac
    41161 ggtggagtgc ccgctacacg ccgccagttt ttgcctgaaa acggggaaag cgttatgcct
    41221 gcccgccacc gatccgctca ccacttatcc agtacacgtt gaaggtggtg acattttcat
    41281 cgacttaccg gaggcgcagc catgagcgat ctgcataacg agtccatttt tattaccggc
    41341 ggcggatcgg gattagggct ggcgctggtc gagcgattta tcgaagaagg cgcgcaggtt
    41401 gccacgctgg aactgtcggc ggcaaaagtc gccagtctgc gtcagcgatt tggcgaacat
    41461 attctggcgg tggaaggtaa cgtgacctgt tatgccgatt atcaacgcgc ggtcgatcag
    41521 atcctgactc gttccggcaa gctggattgt tttatcggca atgcaggcat ctgggatcac
    41581 aatgcctcac tggttaatac tcccgcagag acgctcgaaa ccggcttcca cgagctgttt
    41641 aacgtcaacg tactcggtta cctgctgggc gcaaaagcct gcgctccggc gttaatcgcc
    41701 agtgaaggca gcatgatttt cacactgtca aatgccgcct ggtatcctgg cggcggtggc
    41761 ccgctgtaca ccgccagtaa acatgccgca accggactta ttcgccaact ggcttatgaa
    41821 ctggcaccga aagtgcgggt gaatggcgtc ggcccgtgtg gtatggccag cgacctgcgc
    41881 ggcccacagg cgctcgggca aagtgaaacc tcgataatgc agtctctgac gccggagaaa
    41941 attgccgcca ttttaccgct gcaatttttc ccgcaaccgg cggattttac gggtccgtat
    42001 gtgatgttgg catcgcggcg caataatcgc gcattaagcg gtgtgatgat caacgctgat
    42061 gcgggtttag cgattcgcgg cattcgccac gtagcggctg ggctggatct ttaaggaagc
    42121 acgatgaaag aaaaaaccat cattattgtc ggtggcgggc aagcggcggc aatggctgcg
    42181 gcctcgctac gccagcaagg gttcaccggt gagctgcatc tgttttccga tgagcgacat
    42241 cttccttatg aacgacctcc actctcgaaa tccatgttgc tggaagattc cccgcagtta
    42301 cagcaggtgt tacccgctaa ctggtggcag gaaaacaatg ttcatctgca ttccggtgta
    42361 accatcaaaa cgctgggtcg cgacacacga gagttagtgt taaccaacgg cgaaagctgg
    42421 cactgggatc agctttttat agcaaccggc gcggcagctc gaccgctgcc gttgcttgat
    42481 gcactgggag aacgctgctt taccctacgc catgccggtg atgccgccag actgcgagaa
    42541 gttctgcagc ccgaacggtc agtcgtgatt gtcggtgccg gaactattgg tctggaactg
    42601 gctgccagcg ccacgcagcg cagatgtaag gtgacagtga ttgaactggc ggcaaccgtc
    42661 atgggccgta atgcaccacc gcccgtgcaa cgctatcttt tacagcgcca ccagcaggct
    42721 ggtgtgcgca ttctgctcaa taatgccatt gaacatgtgg tcgatggtga aaaagtagaa
    42781 ctgacgctgc aaagtgggga aacgcttcag gctgatgtgg tgatttacgg tattggtatc
    42841 agcgccaacg agcaactggc tcgcgaggcc aaccttgata ctgccaatgg cattgtcatt
    42901 gatgaggctt gccgcacctg cgatcccgcg atctttgccg gtggcgatgt ggcaatcact
    42961 cgtcttgata atggtgcact acaccgctgc gaaagctggg aaaacgccaa taaccaggcg
    43021 caaattgccg ctgccgcaat gttagggcta ccgctaccgc tactgccgcc gccgtggttc
    43081 tggagcgatc agtacagtga taacttacag tttattggcg atatgcgtgg cgatgactgg
    43141 ctttgtcgtg gcaacccgga aactcagaag gcgatttggt ttaatctgca aaacggcgtg
    43201 cttatcggtg cagtcacgct gaatcagggg cgtgagattc gcccaattcg caaatggatc
    43261 cagagcggca aaacgtttga tgcgaaactg ctgatagatg agaacatcgc gcttaaatca
    43321 ctgtaaccag gataattagc gaatatctca atgcctgggg cgtggcgagg tgcaagagtg
    43381 tgtattacgt ttaaatcaca taatcttgca aagggattgg ttatgaacac actacgttat
    43441 tttgattttg gagctgcccg ccccgttttg ttattaattg cccgtatcgc cgtggtctta
    43501 attttcatta tttttggttt tcccaaaatg atgggctttg acggtacggt ccaatatatg
    43561 gcctcgttgg gcgcgccgat gccgatgctg gcagcgatta ttgcggtagt tatggaagtg
    43621 cccgccgcga tattaatcgt gcttggcttt ttcacccgtc cgctggcggt gctgtttatt
    43681 ttctacacgc tgggtacggc ggtgattggt caccattact aggatatgac cggcgatgcg
    43741 gttgggccaa atatgattaa tttctggaag aatgtcagta tcgctggcgc attcctgctg
    43801 ttggcaatta ccggaccggg ggcgatttct ctcgatcggc gttaggaaag atgccggatg
    43861 cggcatgaac gccttatccg gcattttata aattacagca acgcaacccg caacgacatc
    43921 tctgaagttg ttgattcacc tggtgcaagc gcaatgagat cacccccttc cgggcgatga
    43981 tgatcatccg gcgcatggct catcggttcc agacagaaga aatcaaacgc atatccttta
    44041 tcaaacgcag ggtcggaaac aaagatgaaa taacacggtg caggtggcgt cgtttccatg
    44101 atgatggcat aaccttcctg cggctgctcg atgcgggcct gaccattcca tccggcaaaa
    44161 ccattgttca cccactggcg cggcaacggc gcgggctggt taaaatccag ttcctgcggt
    44221 agctgctcgc aaaactcacc cgccagccac tgctcccgct ccagccagta accgctcgcc
    44281 tgtgcctgaa tccgcgtttg cggcgacaac ggaaaataag gatgccagcc ggtaccaaat
    44341 ggcagcgtct ctgccccttg attggtgaca gagagcgtca ccgtcagcgt atccgccgtt
    44401 aaatgaaacg cctgacttac ccgatagtga tagacaccgc tgcgatgttc atacaccaga
    44461 cacaaactat catcgctgtg cgagacacat tgccactcgc ccagccagcc atcgccgtgt
    44521 agatagtgcg catcccactc aacattcggt tgcagttgat actcacgccc ctgccagaca
    44581 aaacgattgc cgctcacccg gtttgcaaat ggcaccagcg gaaaacatga ggcatcggtt
    44641 gccacaccgc tttttttacc aggacgtaag agcggcgtcg tatcgcgcca gaagccttcg
    44701 attacgccgc cctggtcaga aacgtccagc tttagcgacc cgtgggataa ggtatagatg
    44761 gtcatccgct aactccttaa tccgggaagt taatcacaac tttcccgcat ttgccgctcg
    44821 ccatcagcgc ataggcatca cctgcctgtt ccagcgagaa gcgatgggta atggcgttac
    44881 gcggccacag cttccagtcc gtcagatcat gggcgcattt ttccatatgg aacagactgg
    44941 tcacccagga gccgataatc cgccgttgat ggtgcatcag atcggcgctg acctcgaatt
    45001 ccacttttcc ggtttcacca atgtaaacca cccgtcccca gtcagcggtg gattgcagtg
    45061 ccagcaagcg acctgcggca ttaccggaac aatcgagcgc aacatccgcg ccaccgtggg
    45121 tgagttcggc gataatctgc ggcagacctt cggtggttgc taaatagccg tgatccatca
    45181 cccctaactg ttttgccatc gccagacgtt ccggcagcat atcaacgccg atgatccgtt
    45241 ttgcaccgcg acctttcgcc agcatcatcg ccatcatgcc gaccggcccc agaccgacca
    45301 ccagcacgtt atcgctgccg gaaacttcgc cgcgcaaaat cccttcatag gccgtgccaa
    45361 cgccgcaact gataaacgca ccatcttcgt agctcagcgc atccggcagg aggatcagat
    45421 ctttttcttc cgccagcaga tattcggcat gaccgccgtc acgctgccag ccgtaagccg
    45481 cttttccttt gccagtacaa gagataggaa agccgcggcg gcagttcggg caaaaaccac
    45541 agccagaaat gtgatacacc agcacgcgat caccctcttt aaaatggcga cagccttgcc
    45601 ccatcgccac aatctgcccg cacggttcat gaccgttgat aaatccctgg tataacggtt
    45661 tatcgggtgc cgccgccgtg gcacggtgtt gatgatagat atagtggaca tcgcttccgc
    45721 aaatcccgga ggatttcatt ttgatcagta cctggttaat ccccggcgtc ggcaccgcaa
    45781 cttcccgcag atcgacggtt gaatttcctg gtaaataagc tgccagcatc gttttcataa
    45841 atccctcatt aacaatacga ttaattttca tccctgccgc acccgcgcca ggggcgttaa
    45901 ggtttagcgt ttcgctttac tgcttcgctg ggtcagcaag atattcgcca gcaccgccac
    45961 cacgatgatg acgccgcgta ccacctgctg gaaaaaggag ttaataccga gcagcaccag
    46021 accgttaccg attagcgtaa tcaccagcac accaagcaat gtaccgaaca gggaaccgcg
    46081 accgccggaa agtgccgtac cgccgaccac gaccgcggcg atgacgtcaa actccagacc
    46141 gtttgcggca cctgcgttac cagaaccgag gcgcgccgcc aacaaaatgc cggtcaccgc
    46201 cgctaataat cccgaaaggg taaagataag aatgcgcacc cgacgaacgt tgatgccgca
    46261 caactgcgcc gccgtagcat taccgccaac ggcaaaaacc gagcgcccga aggcggtttt
    46321 gcggctgatg aacacaaaca gcgcaaacaa cacaatcatg atcagcgcgg ataccggcac
    46381 accgagaaat tgtccgccca gccagtccag cacctcgttt tcgtcaatcg gcactggcag
    46441 tgcgttcgtc ataaacagcc ccattccgcg cagggcgctc cacagcccca gtgtggcaac
    46501 gaaacttggt acgttaaaca cgccgcgcag caccccggcc agcgttccca tcagcgcgcc
    46561 taacagcaac accagcagac acgccaccgc cagcggaact tcaaattgca gcaaaaatgc
    46621 caggcacacc gagacaaaag ccaccatcgg cccaacgctg acatcaattt caccggagat
    46681 aataatcagc gtcatcgccc aggcggcaat cccaatggtg gcggcatcgc gcagcacgtt
    46741 catctggtta ttcaatgaga taaagccagg cgcattcagg gagaagacca gataaagaat
    46801 ggcgattacc accagcaaac cgatctcatt aatatggcga ctgacaaatt gtttgagcga
    46861 gacgctcttg ccctgcggca atgataatga cgaagcagac atcttcaggt tcctcggtaa
    46921 aattcatcag tgcacagaca gaatggcgga catcagctca tccacattga ccggcgagtg
    46981 aaactcctgc gagaacgtac cgtgctgtaa caacaggatg cggtcgcaca ccagcggcaa
    47041 ctcctccact tcactggaga taaacaccac gctttttcct tcggcagcca gctcacggac
    47101 aatacggtaa atctgctgtt tggcttcgat atcgacgccg cgcgttggct cgtcgagcaa
    47161 caaaatctgg ctggcggcat agacccaacg accgatcacc actttttgct gattgccacc
    47221 agaaagcgtg ccgatgggcg tttcgctact ggcggccttg accgtcatcc gctgcatcac
    47281 ctcttcggtc aggcggcgga tggtggacca ttgcagcact ccgttggcgc tgattttttg
    47341 ccgattggtc agcactgtat tttcgtcaac gcccaaccag ggaatgatcc ccgcttcttt
    47401 gcggttttct ggcgtatagc caatgccgcg tttcagcatg tcgccgtaat cggggcgcgt
    47461 gattttctcg ccgttgataa caatttcgcc ctgttcatac gtctccagcc caacaatcgc
    47521 cttcagcaat tcactgcgcc ctgcccccag caggccagca atgccaagca cttcgccgcg
    47581 acgtaacgta aagctgatat cctccagctt gggcttatgg cgtaacgcac ggacttccag
    47641 tacggcctga tccacaattt cctgaggggc aaccggcgca atatcaacgt gatcacgccc
    47701 tagcattagc gacacaatat gatgcgtgga ggtgttttcg agcatcacat cgcccgccac
    47761 ctgaccatcg cgcataacgg tggcacagga ggcaatgcgg cggatttctt ccatccggtg
    47821 gctgacataa atcaccgcca cgcccagtgc cgacatcttt ttcaccgcac tgatcaccag
    47881 ttcaacttct gcactggcaa gcgagctggt gggttcatca agaatgacca cgcgcggctc
    47941 gcccttcatc acccgtgcaa tttccaccag ttgcttttgc gccgggctta gcgttgaaac
    48001 aagttgttca ggactaacgt caacgcccag cgcctgtaag caacgttggg catcctgcgc
    48061 catttgcagg taatcaatca tgccgttgcg gcgaggccat tgaccaaggc agaggttttc
    48121 tgccaccgtc agcccttcca ccagacttaa ttcctgataa accgcacgaa cccccagttc
    48181 agcggcacgg cgagtcaacg tagcttcgtc accttccagt cgcgtctcgc caatccagat
    48241 atcaccgcta tccgggcgtt cgctgccggt aagcattcga atgagagtcg atttgcccgc
    48301 gccgttttta cctaacagcg cacgaacttc gcctttattg agcgtgaagt taacgttatc
    48361 cagcgcaacg acgccggggt aacgcttatt tcctgccacc acttttgcta ccgggactgc
    48421 ctctgttgcc gtaaacatag ggacctctgc gaatcagcga ttagggcaga ccatcaacgt
    48481 gcgttgccag ccactgttta ccatcttccg ttttggtata gagatcgatt ggcacctgaa
    48541 tcactttttc accgtcggct tgtttattga taaccttcaa tgtttgcgcg aaaacagcat
    48601 tgcccatttt cttaccggaa atatccacta ccgctttcag cacctgattg ttttccagct
    48661 cctgagcaat ttcggttgtc atatccgaac cgaaaacagc aatttttccg gcctgatttt
    48721 gattacgtac cgcttttacc gcgccgagtg tcgcaccgcc cgactccccc ataatggcgt
    48781 tgagatccgg cgtggagata atcagttttt caccaacgga aatcgcttta tctaaaacag
    48841 tcccttcctg attagcgaca atttgcgcgc cgggaacgcg ggattttaat acttcttcaa
    48901 atcctttacg tcgctgcaca caaacttcaa aggcttcgca attgatgacg gcaattttcg
    48961 gctggtaaat tttattggca ataaaataat cggcggcagc gttacccagt tttttaccaa
    49021 attccagtgg atcgccgacc agatacgccg agacatattt atcgacaccc ttttgattaa
    49081 tacaggtgtt gtagcaaatc accggaatac ccgcttcact ggcgcgacga acggtacggc
    49141 tactgccatt ttcagacact gccgataaaa taatggcatc gacattacgc gccacgaggg
    49201 tatcaacaaa ggtactttct ttcgaaatat caccctgggc gttagtttca attaactgta
    49261 cctgtactga agaatctttt gccgcatcct gaacgccctg acgcactcca gcgtaatatc
    49321 cctgggtatc aaggtatatt gcaccaatgg tcatttcttt atccgcagcc ctggcaaata
    49381 gtgcgctacc taatagcgta gccattaata atagattacg ggttgttctc atttttttag
    49441 gcatagtgcc tcctgtaggg tttttattaa caacggctta ttctaattat tttgtgatga
    49501 gcggcagcgc gtgccgctca aatattacag ttaatagggt ttagtgaacg tagttaaaaa
    49561 taaaaggcat cacggtggtg aataatgccg ctttcggcca ggccggatta atcgccgtca
    49621 attcagcgcg agcagattca tattgcgcaa cctccccgag tcccgcggac gccagcatcg
    49681 ccaccatcag gcatttttct ttatgctgct gttgtaaatc gccatacagc gacaacaggt
    49741 caggttgtga aacagcaaag aaatccgcct caatactggt tttcgccatc tcttgcgccc
    49801 actgtttcat ttcactaaac agttgctgtg cgatttgttg ttcgcccagc agtcgcagcg
    49861 ccattccttg ccagaagaga taatcaaccg gctgatcgtt gtaataactg tggatgttaa
    49921 tggtgcgatc gccggtcgcc gccagacgta aacaacgcgt cgcttcagtt tcatcgccct
    49981 gcgcgttggc gcatatcgcc tgccagaacc agatgtcgtt atcagtttgc cccggtaaac
    50041 ggccttcgct taaattctcc ggataatgca gcgcggcatg aagcagttcg ctggcctgct
    50101 gcggctgtct ggcatcaagg tgctgccagg cgcgtaataa ctggttgagg ataaactgac
    50161 tggtgacctt cccttccccg ccttcccacg ggtggaattt gcgcgtggcg agaatgtccg
    50221 ccgctttgtc tgcctgaccc gtgagatgcc acaaattgag caactctgct gtcatgtcgt
    50281 cgcgtttcag cgcaatttcc agattatttt ccagacgcgc cagtcgtttc tccggtgtgg
    50341 ctccgcttaa cttatcaagc aaatcccgtt cgaaaagcag acgtgcatcc tgcggcgcaa
    50401 gctgataagc attatcaaga taacgcgcgg ccagctcata atcgtgttgc ttattccacg
    50461 catggatcgc taacccgcgc cagccgtcgg caaactccgg cgacatctct acgcaacgtt
    50521 gccataaggt aatggctttg ttgtagctac gcttgttgta gtagaagcag gccagtaaat
    50581 gacgggcaaa ccagcactct tcaatactct ccagcgccgc cacttcttcc agcgtattcg
    50641 ggaaacggac aaactgcggg aagacatcaa tggcttttgc gaccagttcg ccgcgttcgg
    50701 ctttcggcaa taaactcgcc tgcaggtaaa gcggcagcgt gcgctggcag tccagtgcgt
    50761 tcagcatctc tgccgccagg gtgggcattc cccagttaat cagttgcccg gcggtcatca
    50821 gagcgttaac gtcgcgtccc tgacacagac cgcgccactg cgcgagggct gattcgctac
    50881 gaccatcgaa ccagttcagc caccacagag tggcgttcag cggataatca cgcagcagtt
    50941 tcgcgcgctg caaacgcgcg ttgtcctgac gaccacttaa caccagcagc agattatgca
    51001 ggcaaagcac ttcctgattg gttgggcagg cacgaagact ttgttggcaa aaatccagac
    51061 cagcgtcgaa gttaccatta cgcgccgcca gtcgtgccag gccataatag ccaccggctt
    51121 tgctgttgcc gctccagacc gcacgccaga aatcctcttc ggcttgttga tattgtccct
    51181 gacgttcgta agcactggcg cgaatcaaac tggcctgtcc gcactgcgga tttttgttca
    51241 gcgcatgtgc gcgtttcaga gcctgagtgg catacgccac cgcttgcggg aaatcggcgc
    51301 ggttatattc cagcatcgcc agcgccaggt tacagcgata atccagcgga tccagcacca
    51361 cgccgcgcag gtagtaatcg aacggtgaac ggctggcgtg atgatattgc tccagatgct
    51421 gaccgataaa ccaggcttca tctgtactgg taatgtcttg tgctgcgagt ggtgctttgg
    51481 cgacgtccgg caacggcaac tcttgcggct gatgttcctg ataactcagg acaatattgc
    51541 cgtcggcatc ggagagctca atggtcagcc tttcaggatt gataccgtgc aacacgccct
    51601 ggatggcggt cgcaggcatc agtgccacgg catcatcaag taacgcgttg catttgccga
    51661 tttcgcggat cgccaggcgg tatccgttca acggagagat ggcatacagc ccccactcaa
    51721 tcccccgctc actacgctgg agttttatca ccgcatcgcg ggaggcattt tgcaccatgc
    51781 ccaaagaatg ataaggcagg aaatactgct caaaacgctt ctcttcgtaa gcatcaagcc
    51841 aggtaaaatc gggctggtta tcggcaaaaa taccggtcat cagttcgata tacgggccgt
    51901 tattgtcggt cagactctta tcccacgcct ggccaaattc actgtgtccc caactccact
    51961 gttttttacc tggcgcaata tggtggttgg caacgtgcag caaaccgcca tcttcatcgt
    52021 gacaccacgc gccaacaaaa tcgtactgtg atttttcagc catatatgag gttggaacgg
    52081 gcacattttt atagcgagaa atgtccactc cggcggagta gtccactttg tagtaagtgc
    52141 cggtggcgat ggggaaagcg gagacggccc gtttgccgtg atcaaacacc gccgttacat
    52201 ccggcgggaa aacgctctga tgcccttccc cccctttcac tgccgggttg gcccaccaca
    52261 agaaatgacg cggcgtggcg ttaccgttat agacgcggct ggcgatttcc agcgccgccc
    52321 ggtcagggcg cagggtgaaa cctgtcatca cctgtaaacc atgcatcggc tccgtttcgc
    52381 ctacccacac cgtctgtgca ccgtcttcat gggcttcgag ggtgaaatca acgggcataa
    52441 aggtggtcgg gcgatgatgt tgcggccagt taaactcaat cccaccggaa atccacggcc
    52501 ccagcagccc caccagcgca ggtttaatga cttcattgtg ataaacaaaa tcgcgctgtt
    52561 tcactttatc ccacgcgcga tgcacccgac cgcccagttc cggcaggatc atcactttga
    52621 tgtagtcgtt ttccagccac accgcctgcc aggatttcag ggttttctgc tcgctcagcg
    52681 tatcggtcac gccgtaggga taaaccgcgc cggacgatcc ctgataaacg cgattttcca
    52741 ggaacatggg atgtatatcc tgcggcccgg tttcataggt cgggatctca acgcgctctt
    52801 gccacacttt tactggagtc atggtgccct caatattaac aagacatact gaattaaaag
    52861 atttgtggca gtgtattgaa caatctggca atgttttcgc ggaataatca cgcaattaac
    52921 taaacaaggt ttagtgaaga tgagagcctg cattaacaat caacagattc gccaccataa
    52981 caaatgcgtg attctggaac tgctgtaccg gcaaaagcgc gccaataaat caacgctggc
    53041 ccggctggcg caaatttcga ttccggcagt cagtaatatt ttgcaggaac tggaaagcga
    53101 aaaacgggtg gtgaatattg acgatgaaag ccagacgcgc gggcatagta gcggtacatg
    53161 gctgattgcg ccggaaggtg actggacgct gtgcctgaac gtaacgccca ccagtattga
    53221 gtgtcaggtt gctaatgctt gtttaagtcc gaaaggtgaa tttgagtatt tacagattga
    53281 tgcaccgacg ccgcaggcgc tgctgtccga aatcgaaaaa tgctggcatc gtcatcgcaa
    53341 actgtggccg ggccgcacca tcaatctggc gctggcaatc cacgggcagg ttgatccggt
    53401 gaccggcgta tcgcaaacca tgccgcaagc gccgtggaca acgccggttg aggtgaagta
    53461 tctgctggaa gagaagctgg gcattcgggt gatggtcgat aatgactgcg tgatgctggc
    53521 gctggcggag aaatggcaaa ataattcgca ggaacgggat ttctgcgtga tcaacgttga
    53581 ttacggcatt ggctcgtcgt tcgtgattaa cgagcaaatt tatcgcggca gtttgtatgg
    53641 tagcggacag attggtcaca ccatcgttaa tccggatggc gtcgtctgcg actgtggacg
    53701 ttacggctgc ctggaaaccg tcgcctcgtt aagtgcatta aaaaaacagg cgcgggtatg
    53761 gctaaaatca caaccggtta atactcagct tgatcctgaa aaactgacaa ccgcgcagtt
    53821 aatcgctgcc tggcagagtg gagaaccgtg gatcaccagc tgggttgatc gctctgccaa
    53881 tgccattggt ttgagtctgt ataacttcct caacatcctc aatattaatc agatttggtt
    53941 gtacggtcgc agttgtgcct ttggtgagaa ctggcttaat actattattc gccagacagg
    54001 atttaacccg ttcgaccgcg acgaaggacc gagcgtgaaa gcgacgcaaa ttggctttgg
    54061 gcaattaagc cgcgcacaac aggtgctggg aattggctat ttgtatgttg aggcgcagtt
    54121 acgacagatt tgatggcgcg ataacgtaga aaggcttccc gaaggaagcc ttttttcaac
    54181 agattaaccg tttcgcttat gcgtaaaccg ggtaacgtgc gcagatgtcg agaactttac
    54241 ctttgatgcg ctcgataacg gcttcatcat tgatgctgtc cagcacgtca cacatccagc
    54301 cagccagttc tttcgcttct gcttctttga agccgcgacg cgtaattgcc ggagtaccca
    54361 cgcgaatacc ggaggtcaca aacgggctct tcggatcgtt cggtacgctg tttttgttga
    54421 cggtgatgtt agcacggccc agagcggcgt ctgcttcttt accggtcagg tttttatcga
    54481 ccagatcaac caggaacaga tggttatcag taccgccgga aaccactttg tagccgcgtt
    54541 ccaggaacac ttctaccatc gctttagcgt ttttagcgac ctgctgctgg taagttttga
    54601 actcaggctc catcgcttct ttcagagcaa ccgctttacc ggcgattacg tgcatcaacg
    54661 gaccgccctg accaccaggg aaaacggcag agttcagttt tttgtacagc tcttcgctac
    54721 cacctttcgc caggatcagg ccgccgcgcg gacccgccag ggttttgtga gtggtggtag
    54781 taacaacgtg agcatgagga accgggttcg ggtagacgcc agcagcaacc aggcccgcaa
    54841 cgtgcgccat atcaacgaac aggtaagcac cgatgctgtc agcgatttca cgcattttcg
    54901 cccagtccac cacgccggaa tatgcagaga aaccaccgat aatcattttc ggcttgtgtt
    54961 ctttggcttg tttttccaga tcggcgtagt cgatatgacc ggtagcatcg ataccgtaag
    55021 gaacgatgtt gtacagttta ccggagaagt taaccggaga accgtgagtc aggtgaccgc
    55081 catgcgccag gttcataccc agaacggtat cacctggttc cagcagcgcg gtgtagaccg
    55141 caaagttagc ctgggagccg gagtgcggct ggacgttagc gtagtcagcg ccgaacagtt
    55201 ctttcgcacg atcgatcgcc agttgttcaa cgatatcaac atactcgcaa ccgccgtagt
    55261 agcgtttgcc cggataacct tcagcatatt tgttggtcag ctgagaaccc tgcgcctgca
    55321 ttacgcgcgg gctggtgtag ttttcggagg cgatcagttc gatgtgctct tcctgacgta
    55381 ctttttcctg ctccatagcc tgccacagtt cggcatcata atcggcaatg ttcatttcac
    55441 gctttaacat ccgcatctcc tgactcagct aacaataaaa tttttggcct ttataggcgg
    55501 tcctgttgga caacggcgaa cagtataacc gaatcattgt gcgataacag gtcttgacaa
    55561 aggaatttac gcaaacgatt accttcaggc tacgcaaggc tttggagaat aaagagcttg
    55621 caaccggaaa cggatttctt ttcaggtttg tgatgcaaat ttttcacttc atcacattct
    55681 ttctgaaaaa caccaaagaa ccatttacat tgcagggcta ttttttataa gatgcatttg
    55741 agatacatca attaagatgc aaaaaaagga agaccatatg cttgacgctc aaaccatcgc
    55801 tacagtaaaa gccaccatcc ctttactggt ggaaacgggg ccaaagttaa ccgcccattt
    55861 ctacgaccgt atgtttactc ataacccaga actcaaagaa atttttaaca tgagtaacca
    55921 gcgtaatggc gatcaacgtg aagccctgtt taacgctatt gccgcctacg ccagtaatat
    55981 tgaaaacctg cctgcgctgc tgccagcggt agaaaaaatc gcgcagaagc acaccagctt
    56041 ccagatcaaa ccggaacagt acaacatcgt cggtgaacac ctgttggcaa cgctggacga
    56101 aatgttcagc ccggggcagg aagtgctgga cgcgtggggt aaagcctatg gtgtactggc
    56161 taatgtattt atcaatcgcg aggcggaaat ctataacgaa aacgccagca aagccggtgg
    56221 ttgggaaggt actcgcgatt tccgcattgt ggctaaaaca ccgcgcagcg cgcttatcac
    56281 cagcttcgaa ctggagccgg tcgacggtgg cgcagtggca gaataccgtc cggggcaata
    56341 tctcggcgtc tggctgaagc cggaaggttt cccgcatcag gaaattcgtc agtactcttt
    56401 gactcgcaaa ccggatggca aaggctatcg tattgcggtg aaacgcgaag agggtgggca
    56461 ggtatccaac tggttgcaca atcacgccaa tgttggcgat gtcgtgaaac tggtcgctcc
    56521 ggcaggtgat ttctttatgg ctgtcgcaga tgacacacca gtgacgttaa tctctgccgg
    56581 tgttggtcaa acgccaatgc tggcaatgct cgacacgctg gcaaaagcag gccacacagc
    56641 acaagtgaac tggttccatg cggcagaaaa tggcgatgtt cacgcctttg ccgatgaagt
    56701 taaggaactg gggcagtcac tgccgcgctt taccgcgcac acctggtatc gtcagccgag
    56761 cgaagccgat cgcgctaaag gtcagtttga tagcgaaggt ctgatggatt tgagcaaaca
    56821 ggaaggtgcg ttcagcgatc cgacaatgca gttctatctc tgcggcccgg ttggcttcat
    56881 gcagtttgcc gcgaaacagt tagtggatct gggcgtgaag caggaaaaca ttcattacga
    56941 atgctttggc ccgcataagg tgctgtgatt tgatgttgcc ggatggaaac atccggcaac
    57001 ccttgacgcg gtttaaattg ccgcgtcgtc ctcttcaccg gtacggatgc gaatgacccg
    57061 tgccacgtca aagacgaaga ttttaccgtc accgattttg ccggtttgcg ccgtgcgaat
    57121 aatggtatcg acacaggtat cgacaatgtc gtccggtacg acaatctcaa ttttcacttt
    57181 cggcagaaaa tccaccatat actccgcgcc gcggtacagc tcggtatggc ctttctggcg
    57241 accaaagcct ttcacttcgg tcaccgtcat gccggtaata ccgacttcgg ccagtgcttc
    57301 gcggacatcg tccagcttga agggttttat aatcgcatca atctttttca tgctattcct
    57361 tgaaaaggtc gcctgtcttt tgatctgcta aacgtaacac ataacgccaa ttcattcctt
    57421 gaaatcgttt gcatccagct cgtgtcggga aagcagttta taaaattctg tccggttgcg
    57481 ccccgccatt ctcgccgcgt gggtgacgtt gcctttggta atttgcagca gcttacgcaa
    57541 atagttgagt tcaaactgat tacgtgcctc aacaaaggtt ggcagcgccg tgttttcacc
    57601 ctccagcgcc tgctctacta atgcatcgct aataaccgga gatgaggtca gcgccacgca
    57661 ctgttcaatc acgttgacca actggcgcac attacccggc cagctagcgg tcatcaggcg
    57721 tttcatcgca tcagtggaga acgcgcggac aaacggttta tgtcgctctg ccgcctggcg
    57781 caacaggtga tttgccagaa gcggaatgtc ttctgtgcgc tctgccagag ccggaatttt
    57841 caggctgaca acgttgaggc ggtaatagag atcttcgcgg aattccccgc gcgccatcgc
    57901 ttttggcaga tcacggtgag tggcagaaat aatccgcacg tcgatatcaa tatcgcggtt
    57961 gctgcccagc gggcgcactt tacgctcctg caacacgcgc agcagtttga cctgtaacgg
    58021 cgcaggcata tcgccaattt catcgagaaa tagcgttccg ccttccgccg cctggaataa
    58081 accttcgcga ttgctgacag cgccagtaaa cgcgccacgc gcatgaccaa acagctccga
    58141 ctccagcaat tgctcgggca atgcgccaca gttaatagca ataaatggtt tgctgttgcg
    58201 cgggctggcg ttgtggatag cctgggcgaa aatctctttc ccggtgccgc tctgaccgtt
    58261 aatcaaaacg ctgacgtctg attgcgccac cagccgcgcc tgttccagca aacgcagcat
    58321 cagcgggctg cgggtgacaa ttgcctcgcg ccagcgttca tcggtggctg gcgcggattg
    58381 ctccagcgca tcgtcaattg cctgatatag cgcgtctttg tcgacaggct tggtgaggaa
    58441 actaaaaacg ccctgctgtg ttgcagcaac ggcatcgggg atagaaccat gcgcagtaag
    58501 aataattact ggcattcccg gctgcacttt ctggatttca gcaaatagct gcataccgtc
    58561 catttcatcc atccgcaggt cgctgataac taaatctact ttttcgcgat tcagtacccg
    58621 taatccttca gcgccacttt ccgccgtgac cacactgtag ccttcgctgg tcaggcgcag
    58681 gccaagcagt ttcagcaatc ccggatcgtc atcgaccaat aataaatgcg caggtttatg
    58741 gctcatcagg agtgacctca tgggtggatg gcgcgggctt ttcactctcg tgcggcgtat
    58801 ccggcgagaa atttccagcc ggtttgcggg tcgagagctg gcgttcaata tcggtcaggt
    58861 tttccagctt gcgggtggtg agttccagtt gctgctgtag aacgtgatgt tgctggcgca
    58921 atgtatccag ctcgctgtcg ctggactgct ggagtttact gtaacgttgg cgctcttccg
    58981 ccagttgcag ttgcagcgcc tgaccgtcgc gccagagttg atacagtggg cgaacctgtg
    59041 ccgggatctc ggtacttaac gcttcaatac gcgcgaccag ctggcggcgc tcatacggcg
    59101 taattttggc gtcggcgagc aaaatccctt gtttaaaggt attttgccag ctgccgtcgt
    59161 catattggcg agcttgctga cgcgactgcg caggcattaa acgatcagca caatccatcg
    59221 cccgcagcca gtaaagcgga ttggtttcgg ttgatttacc ttgcagcgcc cagatgtcgc
    59281 tacattcagt agaaagatag tcagccagtt gataaaccgg aattttttct tctgctggcg
    59341 tatcaatggc tggcttattg tgattctgca cgcaacccag caatgccaga catggcagcc
    59401 ctgccagcca cagccgtcgg ggcaataatc gttgaaaaat gtgtcgcata ttcaccagac
    59461 ttaaagccta tcccagtggg cgtaattgtt gcagacagtc tggacatgga cagcgcggag
    59521 aaaccggagc gtacatatcg tacgtgaaga tttcgagcac tgcccggggc cgaactgaca
    59581 aataaaatag cctgatagga taggctgtta gcattatttc gtgtttttcg acgacggtaa
    59641 ttcaatgcgg aaacaaacgt cttgcccgct ctcgtcgacc agatacagtt ccccttgcat
    59701 acggcgaata caatccctgg caatgcttaa tcccagaccg ctgcccttca ccgccccttt
    59761 tcgctggtgg cttccctgaa aaaagggttc gaagatcatg gcgcgttcct cttgcggaat
    59821 tggcgtgccg gtattgatga catcaatata aacccgcgca ccatgtgaac tgctgcgaag
    59881 gcaaatgtta ccggattcag ccccgtagtg caccgcattg gagtaaagat tatccagtac
    59941 gctcatcagc agcattggct ccgccaggca agctgttgct ttgagatcga cgtcggtatg
    60001 catcatttta gcccgtgcgg gcaggctatg agcagaaacc actgtctcca ccagcggtgc
    60061 taattcaaca ttctccagtt ccaccgcact gtccgcctgt ttacggttgt aatcaagcag
    60121 ttgttcgatc agtttttgca aattgcggct gctgctatca agaatgctca ccacctcttt
    60181 ttgctctggc gtaagcggcc cgacaacctg gtcagccagt aattcagtgc cctcgcgcat
    60241 actcgccagt ggcgttttta attcatgaga taaatgtctt aaaaattgat ggcgttggga
    60301 ttccagccat gacaggcgct cacttaacca aagaatacgt tgcccaaccg agcgtaactc
    60361 gctcggtcca ctgaacgaaa cgctattgcc cagagaacgc ccttccccca gacggttgat
    60421 catgcgctcg atatttttca ccggcccgat aatcatccgc gtgaaaagta gcaccatcac
    60481 cagactcacc agaaatagca ccagcgattg ccaaccaaaa tattgcccac gttcggcgat
    60541 ttcacgctga agttgctgcc cacgagagaa caccactgtg cgcgtggcct gcaccatttc
    60601 ggtattggca ctggcaaagg cttcaagacg cgcggcggcg gcagcatcgg gaccactgtt
    60661 gttacactga agttgggcca gattgttcaa atcctgacgc aatgcctggt aaagtttatc
    60721 gtctggcagt acgcctgcgt gggcatcgag catttcgctg taacgcttgc gctggctttg
    60781 ataaaccttc gcaagcgtag gatcgtccag cacgcaatac tgacggtagc tacgctccat
    60841 ctccagcgcc gcgttggtca tcgcttcact acgccgggca tcaataagcg tagtgcggtt
    60901 aaccagcgcc gcctgatcgc taagcgcatt caggctttgc caggcttgcc atgccagcac
    60961 caacaggggt agtagaatta gcaaaaatgc cagcattacc agttgtcgta atgagcgggg
    61021 aaaaacaggc cagcgtttca aggtgttact ctcgtcagac gcgaatagcc tgatgctaac
    61081 cgaggggaag ttcagataca acaaagccgg gaattacccg gctttgttat ggaataaggc
    61141 ggtgcctaac tcgacgtttc gcccgatggt tgatatagct acgctgatat cagaagttgg
    61201 acggcaggca ccttgttgtg cgtcattcga attttatgta gcacgtcccg aaggggctga
    61261 cataagtcgg tgaatgagcc actgcttact atatgcagta actgtgccaa taaagaaaat
    61321 agtttggtaa cgtactgatt atacgtcgct ttgaggggtt tatggcttct ccgttatttg
    61381 aatgtaaatc agccactgtg tcgctaaaaa gagacaactt aagataaact tattagataa
    61441 tattaaaatc aatgagttaa gtgtcgccag aaagcgacac ggtaaaccac ccattgtcgt
    61501 gatttacaga cacaaaaaag ctcccggagt tgggagctta tgatagtggt tggtgcttaa
    61561 tgccggatgc ggcgtaaatg ccgcatccgg gctgcaaaac caatgaactg atgacttacc
    61621 ccaactgctt acgcgcattg cggaaaatgc gcatccatgg gccatcctcg ccccagtttt
    61681 ccggatgcca ggagttgctg acagtacgga aaacacgttc cgggtgcggc atcataatgg
    61741 tgactcgacc actttcagtc gtaatcgacg taataccgtt cggtgaaccg ttcgggttag
    61801 ccgggtaggt ttcagtgact ttgccgaagt tatcgacata gcgcagtgcc accagccctt
    61861 tgctttccag tgccgccaga tgcgccgcat cacgcacttc cacgcgccct tcaccatgag
    61921 agacagcaat cggcatttgc gagcccacca tcccctgcaa cagcagcgac gggctttggg
    61981 tcacttcaac caggctgaaa cgcgcttcaa agcgatcgga ggtattgcgc acaaaacgtg
    62041 gccacaactc actacctggg atcagttcgc gcaggttaga catcatctgg caaccgttac
    62101 atacccccag cgccagcgtt tgcggacggt ggaagaaggt tgcaaactca tcgcgtacac
    62161 ggtcattgaa caggattgac ttcgcccaac cttcaccggc acccagcaca tcaccgtagg
    62221 agaaaccacc gcacgcgacc agggcgtgga aatcttccag gcccgtgcgt ccagtcagca
    62281 ggtcactcat atgcacatcg attgcatcga agcctgcacg gtggaaagct gccgccattt
    62341 caacatgcga gttcacgccc tgctcacgca gcacagcaac tttcggacgt gcgccagtgg
    62401 caatatacgg tgctgccaca tcttcgttga tatcgaacga cagtttcaca ttcaggcccg
    62461 gatcggcgtc gtttgatttc gcctggtgct cctggtcagc acactccggg ttgtcacgca
    62521 ggcgctgcat ctgccaggta gtttctgccc accagacacg caacgtggtg cggctttcgc
    62581 tgaatacagt ctgcccgttg gcggtaatca caaaacggtc accggaaacc gcctgcccta
    62641 cataatggac acaatcagca agcccatgct gtgccagtac ggactcgacc gcttcacggt
    62701 cagcggcacg aacctgaatc accgcaccca gttcttcgtt aaacaacgcc gccaggcgat
    62761 cgtcgcccag agaggcaata tccgcgttaa tgccgcaatg accagcaaag gccatttccg
    62821 ccagcgttac cagcaggccg ccatcagagc ggtcgtggta tgcgagcagc ttacgctgtg
    62881 caaccagcgc ctgaatcgcg tcatagaagc ctttcagttg cgcgacatcg cgtacatctg
    62941 ccggtttgtc gccaagctga cgataaacct gcgccagcgc cgttgcgccc agcgcgttat
    63001 tgcctttgcc caaatcaatc agcagcagtg cgttatcttc ggtagaaagc tgcggcgtga
    63061 tggtgtgacg tacatcttcc acgcgggcaa atgcagaaat caccagcgac agcggcgacg
    63121 tcatttcgcg ctcttcgtta ccttcctgcc agcgggtttt catcgacata gagtctttac
    63181 ccaccgggat cgtcaggccc agcgccggac aaagctcttc gcccacggct ttaacggctt
    63241 catacaggcc cgcatcttca ccagggtggc ctgccgccgc catccagttg gcggaaagtt
    63301 taatgcgttt gatatcgcca atctgtgttg cggcaatatt ggttaacgct tcaccgaccg
    63361 ccagacgggc agaggcggcg aaatccagca gcgcaaccgg cgcacgctcg ccaatcgcca
    63421 tcgcttcacc gtagtagctg tcgaggctgg cggtagtgac cgcgcagtta gcgaccggca
    63481 cctgccacgg acccaccatc tgatcgcgcg ctaccatgcc agttacgctg cggtcaccga
    63541 tggtcaccag gaaagttttc tccgccacgg tcggcagatg cagtacgcgt ttcaccgcgt
    63601 ctgcaatggt gatcccttca cggaccagcg catcgccctt cgctttcagc gtttgtacat
    63661 cgcgggtcat cttcggcgtt ttaccaagca ggacgtccag cggcagatcg atcggctgat
    63721 tatcaaaatg acgatcgtgc agagaaagat gcagttcttc ggtcgcttca ccaatcaccg
    63781 cgtagggtgc gcgctcacgc ttacacagtt cgtcaaacag cggtaattga tcggcagcaa
    63841 ccgccagcac gtagcgttcc tgggattcgt tacaccagat ttccagcggg ctcatgcccg
    63901 gttcgtcgct tagaatatcg cgcagttcaa atttaccgcc gcgtccgccg tcgctcacca
    63961 gctccggcat cgcgttagaa agaccgcccg caccgacgtc gtggataaac agaattgggt
    64021 tagcatcacc aagctgccag caacggtcga tcacttcctg gcaacgacgc tccatctccg
    64081 ggttgtcgcg ctgtacggaa gcaaagtcga ggtcggcatc agactgacct gacgccatgg
    64141 aagacgccgc tccgccgcca agaccgatgt tcattgccgg gccgccgaga acgaccagct
    64201 tcgcaccgac gttgatctcg cctttttgta cgtgatcggc gcgaatgttg ccgatcccgc
    64261 ccgccagcat gatcggtttg tgataaccgc gcagctcttc gccgttgtgg ctgttcactt
    64321 tttcttcata agtacggaag tagccattca gtgccggacg accaaattcg ttgttaaacg
    64381 ccgcgccgcc cagcgggcct tcggtcatga tgtccagcgc ggtgacaatg cgctcaggct
    64441 taccgaaatc ttcttcccac ggctgttcga agccaggaat tcgcaggttg gaaacggaga
    64501 aaccaaccaa acccgctttc ggcttcgcgc cgcgcccggt ggcaccttca tcgcggattt
    64561 caccgccgga accggtcgcc gcgcccggcc acggagaaat cgccgtcggg tggttgtgag
    64621 tttcgacttt catcagaata tgcgccggtt cctgatggaa atcgtagcgg cccgtttcgt
    64681 ggtcagcaaa gtagcggccc acttcagaac cttccattac ggcggcgtta tctttataag
    64741 cagagagaac gtaatctggc gtggtttcga aggtgttttt gatcatctta aacagcgatt
    64801 tcggctgttg ctcaccgtcg ataacccaat cggcgttaaa aatcttgtgg cggcagtgct
    64861 cggagttcgc ctgggcgaac atatacagct cgatgtcgtt cgggttacga ccaagctttg
    64921 tgaaagcatc ctgcagatag tcaatttcat cttccgccag tgccaggcca agacgcaggt
    64981 tagcgtcgat tagcgcctga cggccctgcc ccagcaaatc aacgctggtg accggagtcg
    65041 gttgatggtg tgcaaacagc tgctctgcat catctaaagc aaaaaagacc gtttccatca
    65101 tgcggtcgtg cagttcagcg gtaacctgct gccattgttc attggtcagc gtaccggctt
    65161 ctatatagta agcaacgccg cgctcaaggc ggtttacctg ttgtagcccg cagttatggg
    65221 caatatcggt cgctttcgaa gaccagggag agatggtgcc aggacgcggg gtcaccagca
    65281 ggagtttgcc ttgcggggcg tggctggcga gtgccgggcc atatttcagc aggcgttcaa
    65341 gttgtgcgtg ctcatcatcg tttaacggcg cattgaggtc agcaaaatgg acatactcgg
    65401 cgtaaatatt gtgaaccggg agcctggcag cctgaaaacg tgccagcagt ttgttgattc
    65461 ggaatgccga cagtgcaggc gaaccacgca gaatttccat cataagtctc tcgtcttcta
    65521 agctttcggt gtacccaagg gggggaaacg ggcgtcatta taaagaatct gatgcgctga
    65581 cgaaaccgtt tgcgtggaaa taaaatcacc atcgtgaatt agcaacgcgt gccgccaatg
    65641 gctgtaataa gttgccatct ggcgcaggtt tacgcaaaat gccgctcatt tatgagtaaa
    65701 cctttcacta ttattacgtt ttttcaagct gggacgcgca cgacacagag aattaactaa
    65761 ttgaaaaaat taaagattaa ttatctgttc atcggcattc tggcactgct gctcgcggtc
    65821 gctctctggc catccattcc ctggtttggt aaagccgaca accgtatcgc cgccattcaa
    65881 gcgcggggag agttgcgtgt gagcaccatt catactcccc tgacttataa cgaaatcaac
    65941 gggaaacctt ttggcctgga ttacgaactg gcgaaacagt ttgccgatta cctcggcgta
    66001 aaactgaaag tgaccgtgcg gcagaatatc agccagctgt ttgacgacct tgataatggt
    66061 aacgccgacc tgctggcggc aggactggtc tataacagtg agcgggtaaa aaattatcag
    66121 cctggcccta cctattattc cgtgtcacaa caactggttt ataaagtggg tcagtatcgc
    66181 ccacgtacgc tgggcaacct gacggcggag caactcaccg ttgcaccggg tcatgtggtg
    66241 gttaacgatc tccagaccct gaaagaaaca aaattcccgg aattaagctg gaaggtagac
    66301 gacaaaaaag gctctgcgga attaatggaa gatgtcatcg aaggaaaact cgattacacc
    66361 attgctgatt ctgtcgccat cagcctgttt cagcgcgttc acccggagct cgccgtagcg
    66421 ctcgatatca ccgatgaaca accggtgact tggtttagcc cgttagatgg cgataatacc
    66481 ctttccgccg ccctgctcga cttcttcaac gaaatgaatg aagacggtac gctggcacgc
    66541 attgaagaga aatacctggg gcatggcgat gattttgatt acgtcgatac gcgcacattt
    66601 ttacgcgccg tcgatgcggt actgccgcag ttaaagcccc tgtttgagaa atacgccgaa
    66661 gaaattgact ggcgtttgct ggccgctatt gcttatcagg aatcgcactg ggatgcacag
    66721 gccacttcac cgacgggtgt gcgcggcatg atgatgttaa ccaaaaatac cgcgcaaagc
    66781 ctcggcatta cggatcgtac cgatgccgaa cagagcatca gcggtggcgt gcgttatttg
    66841 caggatatga tgagtaaagt gccggaaagt gtgccggaga acgagcggat ctggtttgcc
    66901 ctcgctgcgt acaatatggg ctatgcgcat atgctggatg cccgcgccct gacggcaaaa
    66961 accaaaggga atcctgacag ttgggctgac gtaaaacagc gtctgccttt acttagccag
    67021 aaaccctatt acagcaagct gacttacggc tacgctcgtg gacatgaagc ctacgcttat
    67081 gtcgaaaata ttcgtaagta tcagattagc ctggtgggtt atctgcaaga gaaagagaag
    67141 caggctacag aagcggcgat gcaactggcg caggattatc cggcggtatc gcctacggag
    67201 ttgggcaaag agaaatttcc ttttctctcg tttctttccc agtcgtcatc aaactatttg
    67261 acccattctc cctctctgct gttttccagg aaagggagtg aagagaaaca aaattaatcc
    67321 gtcgaggatt gcgctttttt ctgcgcttta atttcctggc ggcgcatgcg aaagaagtca
    67381 ctgagcaacg ctgcgcactc atccgccagt attccttccg taatttccac tcggtgattc
    67441 atacccggat gatgcagcac atccattaaa gatcccgcag cgccagtttt cgcgtcatgc
    67501 gcaccaaaga ccacgcgacc aatgcgactg tggatcatcg ctccggcaca cattacacat
    67561 ggttcaagcg tgacatacaa cgtggcgtcg atcagacgat aattttgcat caccagacca
    67621 ccctgccgca gggccatgat ttctgcatgt gcggtgggat catggcgacc aatcgggcgg
    67681 ttccagcctt cgccgattac ccgattgtta tgcactaata ccgcgccgac cggcacttcc
    67741 cgctcatccc aggcacgttt cgccagcgtc agcgcgtgac gcatccagta ttcgtggcta
    67801 aattcgactt cagacaaaaa gaaaactccg gttataaaag cgcggcgcat tatacacgga
    67861 cgctatgctt tactctattc cagttgctgg agttcaccgc gcggggttac tcgccaacga
    67921 tgctgacaga aataaagcag cggattgtcc tgattactgt cgctatagcc actgtacagc
    67981 cgcagcggag tgccgatttt gcgctccagt tgtgcgacct tttcatgtcc cagacaacgc
    68041 atcgtcaata cccaaccacc atagccacgc tgaatttggc tggcgataag attaacccgc
    68101 ggcagccagg gcgtatcgaa ataaaccgct tcaaccagcg gctgcggaga gccggtaatc
    68161 aaccagatat cagcatcgga acttaacagg taggtggtta atcgctcctg aaccagcgga
    68221 aaggcggtaa cattgtcgcg aaaccagcgc acgaaatcgg cctgcaacgt ctgtaaacgt
    68281 gcttcgctgt gaccaaaagt gcacccccac agaagcagac tcatcggcca gcgtgccgca
    68341 cgacctttta tcaataacgc aatggctata atcggtaaca acggcaggac aagtaacgca
    68401 ttcagcggtt ggcgacgcag taaatagcgc agaaaactgc cgaacatatc ctgctgatgc
    68461 aatgttccat ctaagtcaaa aaacaccaca cgacgctcgt gagttgccaa accatactcc
    68521 tctggactga aacatcctga ttaattttcg ctactacata gcctaacaga tagatcatca
    68581 cttttccggc aacaatcgac cgcatcatgg ctaactggaa tttttaattc atggcaatta
    68641 gcggcaatgg aatataaaat tcactcgcgt gtgtctcata ttgcctgttg tcgccactat
    68701 ttcatcgcga ggaaagatat gaacggctta cttcgtatcc gtcagcgtta ccaggggctt
    68761 gcccagagtg ataaaaaact ggcggattat ctgctgctac aacctgatac ggcgcgccat
    68821 ttaagttctc agcaactggc caacgaagcc ggagtcagtc agtccagcgt cgtgaagttc
    68881 gcgcaaaaac tcggctataa aggttttccg gcgcttaagt tggcgttgag tgaagcgctg
    68941 gcaagccagc cggaatcgcc ctccgtgccc attcataacc aaatccgcgg tgatgatccg
    69001 ttacggctgg tcggcgaaaa actgattaaa gaaaatactg ccgctatgta cgcaacgcta
    69061 aacgttaata gtgaagagaa actgcatgaa tgcgtaacaa tgttgcgctc tgcgcggcgg
    69121 ataattctga ccggtattgg cgcttcgggt ctggtggcgc aaaactttgc ctggaagctg
    69181 atgaagattg gcttcaatgc tgccgcagtg cgcgatatgc atgcgctact cgcaacagta
    69241 caggcgtcgt cccctgacga tctgttatta gccatttcct acaccggtgt acgacgcgag
    69301 ttaaacctgg cggcagatga gatgctgcga gtgggcggaa aagtgctggc gattaccggc
    69361 tttactccca atgccctgca acagcgtgct tctcattgcc tgtataccat tgccgaagaa
    69421 caagcgacaa acagtgcttc aatctctgct tgtcacgctc agggaatgtt aacggatttg
    69481 ctgttcattg cgctgattca gcaggatctg gaactggcac cagaacgtat tcgtcagagt
    69541 gaagcgctga tgaaaaaact ggtctgagta aagaatgcgc gtataatgcc cgcccggttt
    69601 gtgttgtttt gagagtttcc ttatggcgtt gttaatcact aaaaaatgca tcaattgtga
    69661 tatgtgtgaa cccgaatgcc cgaatgaggc gatttcaatg ggagatcata tctacgagat
    69721 taacagcgat aagtgtaccg aatgcgtagg gcactacgag acaccaacct gccagaaggt
    69781 gtgcccgatc cccaatacta ttgtgaaaga tccggcgcat gtcgagacag aagaacagtt
    69841 gtgggataaa tttgtgctga tgcaccacgc ggataaaatt taatattcta ctctggaagt
    69901 agagtattaa ttatattact gggaagcctt aacgccatta tatttattta attgatgaca
    69961 ttagcataat cattcactaa gttaatttat atagtatctg ccaagacact tatttatagt
    70021 tattaaaggc gcgtccgatt ggttcaccgg acgcacctta agtacgtttc cttgtgttat
    70081 aagaacagaa ggatcagctg taaaacagca atgatgattt tgatgacccg tttaatcagg
    70141 tatcggcaat cagtcattcg tttttcctta aacaaggaat gcagccgttc agattagccc
    70201 ttacatctcc ccaaaactga acgtgcgagt tattgagggt gcatgctgca ctccacacca
    70261 gagctttgac gacaccactc gtttcaatgg gggaattctg tggcatggtg taaagcacag
    70321 caaaatcttc aataacgaag ccaattttaa tgtacttacg aattggcggt cacgttaatc
    70381 tttccatcaa tattgctttc ttcgtaaagg ctcgagtttt tatgctaaag attgcaagtt
    70441 gcttgtaaaa gataagtaca ctgatccata atcgctgttg ttgagggtgc atgctgcaca
    70501 aaattaaagt taaaaagtaa aacccccgtt ccttaccagt tcgggggttt tactttttaa
    70561 agagaacggt attattttaa ctttcaataa ttaccgtggc acaagcatag tggcgttcat
    70621 ctgccagcgt tacatgcata tttacaacgc cgagcttttc cgccagtttt aatgcctcac
    70681 cccatagccg tagccgtggt ttgccgagct catcattgaa tacttcaaat tgattaaacg
    70741 ccagaccatt gcggatcccg gtgccaaacg cttttgctgc ggcttctttc acagcaaaac
    70801 gcttcgccag aaaacgcacc ggctggtggt gcgttttcca gattgcccat tcgttatcgc
    70861 ttaatacgcg gcgtgccagg cgctcaccgg atcgggcgat cactgcttct atgcgagcga
    70921 tctctacgat atccgtgcct aaacctaata ttgccattag ccacgcgctt ccagcatcag
    70981 acgcttcatt tctgccaccg catctttcag tccggtcatc actgcacgac caataatggc
    71041 atgaccgata ttcagttcat gcatctcagg gatggcggca atagctttca cgttgtgata
    71101 ggtcagaccg tgaccagcgt taactttcag accgagacta gcggcaaagg tcgcggcttt
    71161 ggcgatacgc gccagctctt gcgcctgttc ggcgtcagtt ttggcatcag catagcaacc
    71221 ggtgtggatc tcgataaacg gtgcgccaac ctctgccgca gctttgatct gctcttcatc
    71281 ggcgtcgata aagagagaaa cctggatccc ggcatctgcc agacgtttgc aggcatcgcg
    71341 cattttgtca cgctgccctg cgacgtccag gccgccttcg gttgttactt cctgacgctt
    71401 ttccggtacc agacagcaaa aatgtggctt cgtctcaacg gcgatcgcca gcatctcttc
    71461 ggtcaccgcc atctccagat tcatgcgggt atccagtgtc tgacgcagga tgcgcacatc
    71521 acggtcagta atgtggcggc gatcttcacg taaatgcacg gtaatgccgt ccgctcccgc
    71581 ctgctcagca ataaacgccg cctgcaccgg atccgggtaa gcagtaccgc gtgcgttacg
    71641 cagcgttgca atatggtcaa tgttgacgcc taacagtaat tcagccatga caatcctcat
    71701 cattcataat gtgttttcac cgttcgctta ggcataaact gccggaacag ttccctgctc
    71761 tttaatggtt taccgccaag atacggctta agcgccatgc gggtaaagcg tttcgcggcg
    71821 cgtagtgtgt ctgcgtcagg aaattcccgc gcgtttaacg cttttaactg ccttccggtg
    71881 aacgttttat tgtcgataac gacgcttgcg ataaaccctt tttcttcgcg ataacgatac
    71941 gtcatggtgt catctaccgg ctcgccgcta cccgcacaat gggtaaaatt gacgccatag
    72001 cccagatgcc cgagcagtgc cagttcaaag cggcgcagcg cgggttctgg cgtaccagtg
    72061 gcacctgcaa gagactgaat gcagtgcaag taatcgaaaa agagttcaga gaagcgcgtc
    72121 tcgtattcca gtacgcggga gagaagttcg ttgatgtaca gaccgctgta aagcgtgata
    72181 ccgcttaatg gcagcgccag cgagacagct tcagcactgc gcagcgtttt gacttcgcca
    72241 cgcccgccga aacgtagcaa gagaggggtg aaaggctgta atgcaccttt cagagtagag
    72301 cgtttagagc gtgcgccttt ggcaaccaga cgcacgcgac ccgattcctc cgtgaagacg
    72361 tccagcatca ggctggtttc gctccacggg cgactatgca ggacaaatgc gcgctgccag
    72421 ccttccatcg gagtgactct taaagatcgt caacgtaacc gagactgcgc agtgcgcgtt
    72481 cgtcgtcggc ccaaccggat ttcactttta cccacagctc aaggtggaca ggcgcttcga
    72541 acatttcctg catgtcttta cgcgcttcaa tcccgatggt tttgatcttg gcccctttgt
    72601 tgccaataac catcttcttc tgcccttcac gctcaacgag aatcaaacca ttgatgtcat
    72661 aaccaccgcg ttcgttagag acgaaacgtt cgatctccac ggtcacggag tatggcagtt
    72721 cagcgccgag gaaacgcatc agtttttcgc ggatgatttc agacgccata aaacgctgtg
    72781 agcgatcggt gatgtaatct tccgggaagt gatgggtcgc ttcagggagg tgcttacgca
    72841 cgattgctgc gatagtgtcg acattcagcc cggtttcagc agagatcggc acgatatcga
    72901 ggaagttcat ctgacttgcc aggaactgta agtgcggcag cagatcggct ttttcctgca
    72961 cgttatccac tttgttcacc gcgaggatca ccggcgcttt gccatcgcgc agtttgttga
    73021 gcaccatttc gtcgtccggc gtccagcggg tgccttcaac gacaaaaatc accagctcaa
    73081 cgtcgccaat agagctgctc gccgctttgt tcatcaggcg gttaatagcg cgtttttctt
    73141 ccatatgcag gccaggggta tcaacgtaga tcgcctgata cgcgccttca gtatggatcc
    73201 ccacaatgcg gtgacgagtt gtttgcgctt tgcgggaggt gatggagatc ttctgcccca
    73261 gcagtttgtt caacaatgtg gatttgccaa cgttcggacg tccgacgatg gcaataaatc
    73321 cgcagtaact tttatcgatg ctcattccag ctccagtttt ttcaacgcct gttcggcggc
    73381 agcctgctca gccttacgac ggcttgaacc tgtgccaacc accggttcac tcaggccgct
    73441 gacctggcag tggatagtaa attcctgatc gtgcgcttcg ccacgtacct ggactaccag
    73501 ataagtcggc agcggcagat ggcgaccctg caaatattct tgcaagcgcg ttttcggatc
    73561 tttttgttta tcgcctgggc taatttcgtc caaacgagtt tgataccagt tgaggattaa
    73621 tttctcgacg gtttgaatat cactgtcgag gaatacgcca ccaattaatg cttcgacggt
    73681 gtcggcgaga attgactcac gacgaaatcc accgctttta agttcacctg gccctaaacg
    73741 taagcactcg cctaactcaa attcgcgcgc cagttccgcc agcgtattgc cacggaccag
    73801 cgtggcgcgc atccggctca tatcgccttc atccacacga gggaaacggt gataaagcgc
    73861 attggcgata acgtagctca gaatagagtc gcctaaaaat tctaaacgct cgttatgttt
    73921 actgctggca ctacgatgag ttaatgcctg ctgcaacagt tcctgatgat taaaagtgta
    73981 gcccagcttc cgttgaagcc gattaattac gatggggttc atgcgatacc aataaataaa
    74041 tgcgtcaaca attcagcaca cgaaacagac ctgatataca tggctctgct aactgcttcg
    74101 ctgcagtttc agtttaccgg tatatgggga ccaacgctgt ttcgtgtgcc gtggcaacct
    74161 ggaggtgcca accttaaact tcgggggaat attctataca caacgacggg ggatgtcgtt
    74221 agccacggga gatttatctc ataaataatt cacgttgtcg ccataacggc gacaacgtga
    74281 acgaagatga gctattaatg gatgccgcca atgcgactta agcgcacacc agtcggccat
    74341 tcgccttctt gcttatcgaa gctcatccag atagccgttg cccgaccgac cagattcgct
    74401 tcaggcacaa acccccaata acggctgtcc gcgctgttgt cgcggttgtc gcccatcatg
    74461 aagtattgtc ccggaggaac aatccaggtt gccagttgtt gccctggctg ctggtaatac
    74521 atccccacct gatcctgcgc aatcggcact gtcagaattc ggtgcgtcac atcacccagt
    74581 gtctctttac gctcggaaag acgaattcca ttttctttgg tttcgttttt cggcacttca
    74641 aagaatccgc tggtcgcttc cccaccatta cggcgtgaga aggtctgaac gaaatcgctc
    74701 ggttccacgt ttgagtaggt gaccggcagc gcgttttcac acgcctggcc ggaactgcat
    74761 cccggttgaa tcgtcagctc tttcgagacc ggatcataag taactttatc gcccggtaaa
    74821 cccaccgcgc gcttgatgta atcaagcttt ggatcttccg gatatttaaa gaccacgata
    74881 tctccgcgtt tcggatgacc ggtttcgatc agcgttttct ggtagatagg atctttaatg
    74941 ccataagcaa acttttctac cagaataaaa tcaccgatta acagagtcgg catcatcgaa
    75001 cctgacggga tctggaacgg ttcataaata aacgaacgca caatcaatac gatagccagt
    75061 accggaaaaa cagaagcacc ggtttccagc cagccaggct tcggcgcaac ctttttcaac
    75121 gttgctttat ccagtgagtc accggcagcc gcctgcgccg ctgcctgacg ttcccgccgt
    75181 ttaggtgcga aaaagaattt atccacgcac cataaaatgc ccgtcaccag tgtggcaatc
    75241 accagaatca gggcaaacat attcgccatg ccaactccta agggttattt gttgtctttg
    75301 ccgacgtgca gaatggcgag gaacgcttcc tgcggcagct cgacgttacc gatctgcttc
    75361 atgcgtttct taccttcttt ctgcttctgc agcagctttt tcttacggct gatatcgccg
    75421 ccataacatt tagccagtac gtttttacgc agctgtttca cggtggatcg cgcaataatg
    75481 tgcgtaccaa tcgctgcctg aatggcgata tcaaactgct ggcgtgggat cagatctttc
    75541 atcttctcca ccaactcgcg accgcggttt tgcgaattat cacggtgggt gatcagcgcc
    75601 aacgcatcaa cacgttcacc gttgattaat acgtctacac gtaccatgtc ggacgcctgg
    75661 aagcgtttga agttgtaatc cagagacgca taaccacgcg aggtagattt caggcgatcg
    75721 aagaagtcga gcaccacttc cgccatcggg atctcgtacg tcagcgccac ctgattaccg
    75781 tggtaaacca tattggtctg cacgccgcgt ttttctacgc acagcgtaat aacgttgccg
    75841 agatatgcct gcggcagcag catgtgacat tcggcaatcg gctcgcgcag ttcgtagatg
    75901 ttatttaccg ctggcagctt ggatggactg tcgacgtaaa taacttctct tgacgtggtt
    75961 tcaacttcat acactacggt cggcgcagtg gtgatcagat ccagatcgta ttcacgttcc
    76021 agacgttcct ggatgatctc catgtgcagc aggccgagga agccgcagcg gaaaccaaag
    76081 cccagcgcgc tggagctttc cggctcatag aacagtgaag catcgttcag gctgagttta
    76141 cccagcgcgt cacggaaggc ttcatagtcg tcggaactta ccgggaacag accggcgtat
    76201 acctgcggtt tgactttctt aaagccaggc agcgcctttt ctgccggatt acgcgccagc
    76261 gttaaggtat cgccgactgg agcgccgtgg atatctttaa tcgcacatac cagccagcct
    76321 acttcgccac atttcagttc agtgcggtca acctgtttcg gcgtgaagat gcccagacgg
    76381 tcggcgttat aggtctgccc ggtactcatg actttcactt tgtcgccctt acgcagggtg
    76441 ccgtttttaa tacggataag tgaaacaacg cccaggtagt tgtcgaacca tgagtcgata
    76501 attagtgcct gcaacgggcc ttccggatcg ccttccggcg gcggaatgtc gcgcaccaga
    76561 cgttcgagaa cgtcctgcac gccaacgccg gttttcgctg aacagcgcac cgcgtcagtg
    76621 gcgtcgatgc cgacgatatc ttcaatttct tccgccacgc gttcaggatc ggctgccggc
    76681 aggtcaatct tgttcagtac tggcaccact tcgagatcca tttccatggc ggtgtagcag
    76741 tttgccaggg tttgcgcttc cacgccctgc ccggcgtcga ccaccagcaa tgcaccttca
    76801 caggcagcca gcgaacggga aacttcatag gagaagtcta cgtggcccgg ggtgtcgata
    76861 aagttaagct gataggtttc gccgtcagac gctttgtagt ccagcgtcac gctttgcgct
    76921 ttgatggtaa tgccacgctc acgctcaaga tccatggaat cgagaacctg cgcctccatt
    76981 tcacggtcag acaggccacc gcagatctgg ataatacggt cagacagcgt cgatttaccg
    77041 tggtcaatgt gagctatgat cgaaaagtta cgtatattct tcataaagta tgattattgt
    77101 gccttaatgc ccggttaacc aggcttttag aagtcgctgt tctgagctta acgtctgtat
    77161 taatagaaac gccgcattct acactacaac attgaggcga ggaaatgttc ataccgtatg
    77221 gattgtggta tctggaaacg tcctcgcatt tgttatgcaa aatgcaacaa agccagtgaa
    77281 atcactggct cgcgtcttcc gaagatgttt caaatcgcac aaggccaggc ggcaatgcca
    77341 cgcttaagat gatcggttgc cattctgccc gggcagcaaa cttacgcgag tagccgcgcg
    77401 caatcaggaa cccgccaata ccaccgagga tcgcaccaca taatgctgcg acgtcagaag
    77461 caaagagtag ctgaaatagc gaagcgataa ggaataatcc caccagcggc gacatataaa
    77521 ccagtaatgc ggagctaagc aggctgcctt cggcgatccc caattccact ttttgccccg
    77581 gcactaacgg ttcatcacag ggtacgacaa tggtatgcgt ggtttgcggg ccaagtttat
    77641 tcaacacgcg gctaccgcaa ccggcgcgtg aagcgcagct gctgcatgaa gctttaacat
    77701 cacaactgac cagcgcctgc ccgttttgcc aggagacgac ggtagcccac tctttgatca
    77761 ttgcgctgcc ccgaacttaa tattctcggc aatgcgtttc gccgtttgcg gcggcagttc
    77821 accgacaatg gtgatttcgg cgttatcacg tacgcttgta ctgacggttc tgcgtccggt
    77881 gcgcaacatc tgatcggtgc tcgatggcgt agcgcggtta acgtttaccg agaagctgaa
    77941 taatccgtcg gaatagagac gtgattcgat aggcatgttg tccatcgtcg gtagcggacg
    78001 tcgactactg gaaacttcgc taaaaccctg tggcaaccag gttggcgtcc agctgaattt
    78061 agctttttca cctacaggaa cagaaagcaa cggcggcaaa tttgccttcg ccagcgtctg
    78121 catactgcta ctgatatcct gattgacgtt aaaagcaatc acgcgaaatt gttccagcgt
    78181 ttcaccatcg cgatcaagga gatcaaccct catcggtaat ttcgattcga tgtccatcca
    78241 cacgatgtag ctgtagcgtg taccatctcg ggcaaccacg cgaatgactt cgcaaagacg
    78301 atcagcaata cgcgtgcgcc cgacggagat aaagtcgtag taaggagaaa ggcgtttgaa
    78361 atcggtatag atgagcgatg gcagagaatc aacaatgtaa tcgccattaa gcgtgaacgg
    78421 ttcaagtccc ggttcaaaat agctgatttc attgccgcgc tgtaccactt cccggcgcgg
    78481 gccatccatt tgcaacaatt gtgcaagagg acggttatcg aggcgtgcat gtcgataacg
    78541 cagagactca acaccctgtt tattgatgct gatgaatgac agctcgtaat tcagtgactg
    78601 actggccagg ttcatctgct gtaataacgc cccggacgcg ggagtggccg aggcgttagc
    78661 agagaataac aggctacctg tcactaatga catggcaaac caaagttgct tcattactgc
    78721 gattgcgttc ctaaagtttg aattcctggc acctgtacag cggcttgctg ggtttgcgcc
    78781 tgctcaaact gaagctgttc agagtggagt cggcgttgca gttcgtaatc ctgcaacatt
    78841 gcattaatgc gacgacgctg ctcctgtacc tgctgctgtt gaccattgtt tgcggtcgct
    78901 tcagaaggta ctcccaggct taccgggctg gctttaccca tcatcggcag tgtattaaat
    78961 accggcgttt cgggctgctg ggacgtttca gattgtccat tatagtgctg gacgccaacg
    79021 ataactgcaa gcgatacgca tgctgctacg cccatttggg taagctgtgc cgcccacgga
    79081 cgtactttct gccagaatgg cattttctgc cattgatgcg gcgcaggctg ggcttccggg
    79141 atcaatgtcg ccggttgacg tactggctct tcttcaatgg cggccatcac gcgtgaagag
    79201 atatcgaaat ggagcacctc gggagtatca ccccgcattg agtcacggat taagtgatag
    79261 ctttcccagg ttttctgcat ttctgggtta tgagccagtt cgttaagcag ctcactatcc
    79321 agcgtttcgc catccattaa agcggaaagt tgttctttct gcatgcctaa tacccttatc
    79381 cagtatcccg ctatcgtcaa cgcctgataa gcggttgaac tttgttatca atagcttccc
    79441 tcgctcggaa gatacgtgaa cgcaccgtac ctaccggaca atccatgata gcggctatct
    79501 cttcatagct caggccatcc agctcccgca aggttattgc catgcgcaaa tcttccggga
    79561 gggactcaat agttcggaaa actatctgtc tcagttcttc tgacaacatt aagttctcag
    79621 ggttcgaaat ttctttcaac gcgccgccac tttcgaagtt ttcagcttca atggcatcca
    79681 catcactgga aggtggacga cgcccctgag caaccaggta atttttcgct gtatttacag
    79741 caatccgata cagccatgta taaaaagcgc tatctccccg gaacgaatcc agcgcacgat
    79801 aggctttaat aaaagcttct tgtaccacat cgggaacatc acccgacggc acatagcggg
    79861 aaaccagact cgccacttta tgctgatagc gcactaccag taggttaaag gctttctgat
    79921 ctcccttctg gacccgttca accaggacct ggtccgttaa ctgctcgctc atccgaggta
    79981 aagtctcccc aaaccaaatt tccacgcgct atcgaaacgc cactccatta gctgcaattt
    80041 gagcaagcaa agggttagag tgtctcgttt ttgtaaagtt ccgtaacgca tctgtttttg
    80101 tttgtcatgc tgtagacgga tcattatcta tcattataag tctacagaat ctgaacatcg
    80161 cattatctgt gtagaaatgc ccatttaact gcctgaagag taacccaacg gcctttttat
    80221 ttcaccacct aatcctccac cagccagtaa cttctctttt tctcgccgcc ctgcgtcagc
    80281 gtgtttagcc actgtaacaa atattaaaat agcaggtgtt tattcgcaca acatgatgct
    80341 atgctgacca aacggtgttt agtaaattaa acaaagaaaa tgaatactct cccagaacat
    80401 tcatgtgacg tgttgattat cggtagcggc gcagccggac tttcactggc gctacgcctg
    80461 gctgaccagc atcaggtcat cgttctaagt aaaggcccgg taacggaagg ttcaacattt
    80521 tatgcccagg gcggtattgc cgccgtgttt gatgaaactg acagcattga ctcgcatgtg
    80581 gaagacacat tgattgccgg ggctggtatt tgcgatcgcc atgcagttga atttgtcgcc
    80641 agcaatgcac gatcctgtgt gcaatggtta attgaccaag gggttttgtt tgatacccac
    80701 gttcaaccga atggcgaaga aagctaccat ctgacccgtg aaggtggaca tagtcaccgt
    80761 cgtattcttc atgccgccga cgccaccggt agagaagtac aatccacgct ggtgagcaag
    80821 gcgcagaacc atccgaatat tcgtgtgctg gagcgcagca acgcagttga tctgattgtt
    80881 tctgacaaaa ttggcctccc gggcacgcga cgggttgttg gcgcgtgggt atggaaccga
    80941 aataaagaaa cggtggaaac ctgccacgca aaagcagtgg tactggcaac cggcggtgcg
    81001 tcgaaggttt atcagtacac caccaatccg gatatttctt ctggcgatgg cattgctatg
    81061 gcgtggcgcg caggctgtcg ggttgccaat ctcgaattta atcagttcca ccctaccgca
    81121 ctgtatcacc cacaggcacg caatttcctg ttaacggaag cactgcgcgg cgaaggcgct
    81181 tatctcaagc gcccggatgg cacgcgtttt atgcccgatt ttgatgagcg cggcgaactg
    81241 gccccgcgcg atattgtcgc ccgcgccatt gaccatgaaa tgaaacgcct cggcgcagat
    81301 tgtatgttcc tcgacatcag ccataagccc gccgatttta ttcgccagca tttcccgatg
    81361 atttatgaaa aattgctcgg gctggggatt gatctcacac aagaaccggt accgattgtg
    81421 cctgctgcac attatacctg cggtggtgta atggttgatg atcatgggcg tacggacgtc
    81481 gagggcttgt atgccattgg cgaggtgagt tataccggct tacacggcgc taaccgcatg
    81541 gcctcgaatt cattgctgga gtgtctggtc tatggctggt cggcggcaga agatatcacc
    81601 agacgtatgc ctgatgccca cggcgtcagt acgttaccgc cgtgggatga aagccgcgtt
    81661 gagaaccctg acgaacgggt agtaattcag cataactggc acgagctacg gctgtttatg
    81721 tgggattacg ttggcattgt gcgcacaacg aagcgcctgg aacgcgccct gcggcggata
    81781 accatgctcc aacaagaaat agacgaatat tacgcccatt tccgcgtctc aaataatttg
    81841 ctggagctgc gtaatctggt acaggttgcc gagttgattg ttcgctgcgc gatgatgcgc
    81901 aaagaaagcc gggggctgca ttttacgctg gattacccgg aactgctcac gcattccggt
    81961 ccatcgatcc tttcccccgg caatcattac ataaacagat aaaaagcctg ggtcagcgcc
    82021 gtatacgctt cggaatagtt ctggtctggc ccacgaatga ctaagcgatc gctaaagcat
    82081 tctcccgcct gcggggagaa tgccagcagc acccgatgcg gcagtcgcgc ttcgttttcc
    82141 gccacatccg tccgcaaacg taaatgccag cccatgctta atgccagttc agtaaaacca
    82201 ttaccaatct gctctggcag caccacgcag aaaaatccct cttcggtaat gcactccgcc
    82261 gcacaggtca gcagcgatgg gtgatcaagc gtagtggtat agcgagcctg ttcccgttga
    82321 ggtgtcgcgc actctactcc ctgctgatag taaggtgggt tactgatgat taaatcgaag
    82381 cgtactgtct gctgtgtgat ccactgctga atatccgccg tatggacgtt aatccgctct
    82441 gcccacgggg actggttgat attttcctgc gcctgcgcgg cagcttcact ttccagctca
    82501 acggcatcaa ttatcacgct gtcactggtt cgctgcgcca gcatcagtgc cagcaacccg
    82561 ctacccgcgc cgatatcaag gcaacgtttc accccagcca ccggtgccca tgcgcccagc
    82621 aaaatgccat ccgttcccac tttcatcgca cagcgatcgt gagcaacaaa aaactgttta
    82681 aaagtaaatc cattacgacg aagcacggat gtagactgtg acatgaaaat aaaaccttgc
    82741 aggaaaaacg gcgatagcac cgggtgagaa caatacccga gaagcgatat ccatacaaac
    82801 agatgaagat tgcagccgta acgtctataa tcagcgcccc acacagaggt agaacatgac
    82861 tgtaacgact ttttccgaac ttgaactcga cgaaagcctg ctggaagccc tccaggataa
    82921 aggtttcact cgcccgaccg ccattcaggc tgccgccatt ccgcctgcgc tcgatggccg
    82981 tgatgtactc ggttctgcgc cgacaggcac cggtaaaacg gcggcgtatc tgctgccagc
    83041 gttgcagcac ctgctcgatt tcccgcgtaa gaaatccggt ccgccgcgta ttttgatcct
    83101 caccccaact cgcgagctgg cgatgcaggt ggccgatcat gcccgcgaac tggcgaaaca
    83161 tacgcatctg gatatcgcca ccatcaccgg cggcgtagcc tatatgaacc acgcggaagt
    83221 gttcagcgaa aatcaggaca tcgtggtcgc cacgaccgga cgtctgctgc aatacataaa
    83281 agaagagaac ttcgattgcc gcgcggttga aacgctgatc ctcgacgaag cagaccgtat
    83341 gctggatatg ggcttcgctc aggatatcga acatattgct ggcgaaacgc gctggcgtaa
    83401 acagaccctg ctcttttcgg caacgctgga aggcgatgcg attcaggact ttgccgagcg
    83461 tctgctggaa gatccggtgg aggtttctgc caatccgtcc acccgtgagc gcaaaaaaat
    83521 tcatcagtgg tattaccgcg ccgatgacct ggaacataaa accgcattgc tggtgcatct
    83581 gttaaaacag ccggaagcga cccgctcaat tgtgtttgtg cgtaagcgtg aacgtgtgca
    83641 tgagctggca aactggctgc gcgaagcggg catcaacaac tgctatctcg aaggtgagat
    83701 ggtacaaggc aagcgtaacg aagcgatcaa gcgtttgacc gaaggtcgcg taaacgtact
    83761 ggttgcaacc gatgttgccg cgcgcggtat cgacattcct gacgtcagcc acgtctttaa
    83821 cttcgatatg ccgcgcagtg gcgatactta tttgcaccgt atcggacgta ccgcgcgcgc
    83881 cggtcgtaaa ggcaccgcaa tttcgctggt ggaagcccat gaccatttgc tgctgggcaa
    83941 ggttggccgt tacattgaag agccgattaa agctcgcgtt attgatgagt tacgcccgaa
    84001 aacgcgtgcg ccaagcgaga agcagaccgg caagccatcg aagaaagtac tggcaaaacg
    84061 cgccgagaag aaaaaagcta aagagaaaga gaaaccgcgt gtgaaaaaac gccatcgcga
    84121 caccaaaaat attggtaagc gccgtaaacc aagcggaacg ggcgtgccac cgcaaacgac
    84181 agaagagtaa tctcaatgcc gggtttaagc ctggtattaa aagtgctggc aaacgcaaaa
//
LOCUS       DAZFXT010000005        82446 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.005, whole genome shotgun sequence.
ACCESSION   DAZFXT010000005 DAZFXT010000000
VERSION     DAZFXT010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82446
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.005"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..1680)
                     /gene="yojI"
                     /locus_tag="ACHKW0_000355"
     CDS             complement(37..1680)
                     /gene="yojI"
                     /locus_tag="ACHKW0_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HFO7473249.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTNVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDRLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKFELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(1756..2406)
                     /gene="alkB"
                     /locus_tag="ACHKW0_000356"
     CDS             complement(1756..2406)
                     /gene="alkB"
                     /locus_tag="ACHKW0_000356"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HFO7473250.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(2406..3470)
                     /gene="ada"
                     /locus_tag="ACHKW0_000357"
     CDS             complement(2406..3470)
                     /gene="ada"
                     /locus_tag="ACHKW0_000357"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HFO7473251.1"
                     /translation="MKKATCLTDDQCWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPEKANAQQHRLDKITHACRLLEQETPVTL
                     EALADQVVMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRSLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLMFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIIIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(3544..4599)
                     /gene="apbE"
                     /locus_tag="ACHKW0_000358"
     CDS             complement(3544..4599)
                     /gene="apbE"
                     /locus_tag="ACHKW0_000358"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HFO7473252.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSTLMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALDSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            complement(4711..5814)
                     /gene="ompC"
                     /locus_tag="ACHKW0_000359"
     CDS             complement(4711..5814)
                     /gene="ompC"
                     /locus_tag="ACHKW0_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HFO7473253.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSSK
                     RTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            6552..9224
                     /gene="rcsD"
                     /locus_tag="ACHKW0_000360"
     CDS             6552..9224
                     /gene="rcsD"
                     /locus_tag="ACHKW0_000360"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009927 - histidine phosphotransfer kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HFO7473254.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            9241..9891
                     /gene="rcsB"
                     /locus_tag="ACHKW0_000361"
     CDS             9241..9891
                     /gene="rcsB"
                     /locus_tag="ACHKW0_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor RcsB"
                     /protein_id="HFO7473255.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(10091..12940)
                     /gene="rcsC"
                     /locus_tag="ACHKW0_000362"
     CDS             complement(10091..12940)
                     /gene="rcsC"
                     /locus_tag="ACHKW0_000362"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0018106 - peptidyl-histidine
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HFO7473256.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(13215..13991)
                     /gene="yfaP"
                     /locus_tag="ACHKW0_000363"
     CDS             complement(13215..13991)
                     /gene="yfaP"
                     /locus_tag="ACHKW0_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HFO7473257.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(13996..15645)
                     /gene="yfaQ"
                     /locus_tag="ACHKW0_000364"
     CDS             complement(13996..15645)
                     /gene="yfaQ"
                     /locus_tag="ACHKW0_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HFO7473258.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(15646..20160)
                     /locus_tag="ACHKW0_000365"
     CDS             complement(15646..20160)
                     /locus_tag="ACHKW0_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HFO7473259.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELRLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDGPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            complement(20184..20807)
                     /gene="yfaT"
                     /locus_tag="ACHKW0_000366"
     CDS             complement(20184..20807)
                     /gene="yfaT"
                     /locus_tag="ACHKW0_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HFO7473260.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(20804..22492)
                     /gene="yfaA"
                     /locus_tag="ACHKW0_000367"
     CDS             complement(20804..22492)
                     /gene="yfaA"
                     /locus_tag="ACHKW0_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HFO7473261.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDDTLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            complement(22641..25268)
                     /gene="gyrA"
                     /locus_tag="ACHKW0_000368"
     CDS             complement(22641..25268)
                     /gene="gyrA"
                     /locus_tag="ACHKW0_000368"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HFO7473262.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            25415..26137
                     /gene="ubiG"
                     /locus_tag="ACHKW0_000369"
     CDS             25415..26137
                     /gene="ubiG"
                     /locus_tag="ACHKW0_000369"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061542 - 3-demethylubiquinol-n
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HFO7473263.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNTFKLGPGVDVNYMLHTQNK"
     gene            complement(26265..29999)
                     /gene="yfaL"
                     /locus_tag="ACHKW0_000370"
     CDS             complement(26265..29999)
                     /gene="yfaL"
                     /locus_tag="ACHKW0_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HFO7473264.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGFAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGDGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLKNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(30424..30567)
                     /gene="ypaB"
                     /locus_tag="ACHKW0_000371"
     CDS             complement(30424..30567)
                     /gene="ypaB"
                     /locus_tag="ACHKW0_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001753443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpaB"
                     /protein_id="HFO7473265.1"
                     /translation="MALLQVHNFVDNSGRKKWLSRTLGQTRCPGKSMGREKFVKNNCS
                     AIS"
     gene            30695..32980
                     /gene="nrdA"
                     /locus_tag="ACHKW0_000372"
     CDS             30695..32980
                     /gene="nrdA"
                     /locus_tag="ACHKW0_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1a ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HFO7473266.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGVRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            33127..34257
                     /gene="nrdB"
                     /locus_tag="ACHKW0_000373"
     CDS             33127..34257
                     /gene="nrdB"
                     /locus_tag="ACHKW0_000373"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0009263 - deoxyribonucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class Ia ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HFO7473267.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            34257..34511
                     /gene="yfaE"
                     /locus_tag="ACHKW0_000374"
     CDS             34257..34511
                     /gene="yfaE"
                     /locus_tag="ACHKW0_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HFO7473268.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(34565..35215)
                     /gene="inaA"
                     /locus_tag="ACHKW0_000375"
     CDS             complement(34565..35215)
                     /gene="inaA"
                     /locus_tag="ACHKW0_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HFO7473269.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGNAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(35295..35399)
                     /locus_tag="ACHKW0_000376"
                     /pseudo
     CDS             complement(35295..35399)
                     /locus_tag="ACHKW0_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            35397..35636
                     /locus_tag="ACHKW0_000377"
                     /pseudo
     CDS             35397..35636
                     /locus_tag="ACHKW0_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(35678..36754)
                     /gene="glpQ"
                     /locus_tag="ACHKW0_000378"
     CDS             complement(35678..36754)
                     /gene="glpQ"
                     /locus_tag="ACHKW0_000378"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7473270.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(36759..38117)
                     /gene="glpT"
                     /locus_tag="ACHKW0_000379"
     CDS             complement(36759..38117)
                     /gene="glpT"
                     /locus_tag="ACHKW0_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015169 - glycerol-3-phosphate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HFO7473271.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            38390..40018
                     /gene="glpA"
                     /locus_tag="ACHKW0_000380"
     CDS             38390..40018
                     /gene="glpA"
                     /locus_tag="ACHKW0_000380"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046174 - polyol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HFO7473272.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVAAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            40008..41267
                     /gene="glpB"
                     /locus_tag="ACHKW0_000381"
     CDS             40008..41267
                     /gene="glpB"
                     /locus_tag="ACHKW0_000381"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HFO7473273.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            41264..42454
                     /gene="glpC"
                     /locus_tag="ACHKW0_000382"
     CDS             41264..42454
                     /gene="glpC"
                     /locus_tag="ACHKW0_000382"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit GlpC"
                     /protein_id="HFO7473274.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            42648..43550
                     /gene="rpnE"
                     /locus_tag="ACHKW0_000383"
     CDS             42648..43550
                     /gene="rpnE"
                     /locus_tag="ACHKW0_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HFO7473275.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(43591..44052)
                     /locus_tag="ACHKW0_000384"
                     /pseudo
     CDS             complement(43591..44052)
                     /locus_tag="ACHKW0_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
     gene            complement(44116..45318)
                     /gene="yfaY"
                     /locus_tag="ACHKW0_000385"
     CDS             complement(44116..45318)
                     /gene="yfaY"
                     /locus_tag="ACHKW0_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HFO7473276.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGMVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            complement(45418..45960)
                     /gene="yfaZ"
                     /locus_tag="ACHKW0_000386"
     CDS             complement(45418..45960)
                     /gene="yfaZ"
                     /locus_tag="ACHKW0_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaZ family outer membrane protein"
                     /protein_id="HFO7473277.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     GGNRDNAVADGPYVGVNASF"
     gene            46239..46664
                     /gene="nudI"
                     /locus_tag="ACHKW0_000387"
     CDS             46239..46664
                     /gene="nudI"
                     /locus_tag="ACHKW0_000387"
                     /EC_number="3.6.1.12"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HFO7473278.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEIMPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVAARKTLRLKGLL"
     gene            complement(46703..47305)
                     /gene="ais"
                     /locus_tag="ACHKW0_000388"
     CDS             complement(46703..47305)
                     /gene="ais"
                     /locus_tag="ACHKW0_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II)-phosphate
                     phosphatase Ais"
                     /protein_id="HFO7473279.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNY"
     gene            47613..48752
                     /gene="arnB"
                     /locus_tag="ACHKW0_000389"
     CDS             47613..48752
                     /gene="arnB"
                     /locus_tag="ACHKW0_000389"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HFO7473280.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            48756..49724
                     /gene="arnC"
                     /locus_tag="ACHKW0_000390"
     CDS             48756..49724
                     /gene="arnC"
                     /locus_tag="ACHKW0_000390"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HFO7473281.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            49724..51706
                     /gene="arnA"
                     /locus_tag="ACHKW0_000391"
     CDS             49724..51706
                     /gene="arnA"
                     /locus_tag="ACHKW0_000391"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HFO7473282.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRIAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKMIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            51703..52593
                     /gene="arnD"
                     /locus_tag="ACHKW0_000392"
     CDS             51703..52593
                     /gene="arnD"
                     /locus_tag="ACHKW0_000392"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HFO7473283.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            52593..54245
                     /gene="arnT"
                     /locus_tag="ACHKW0_000393"
     CDS             52593..54245
                     /gene="arnT"
                     /locus_tag="ACHKW0_000393"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HFO7473284.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPQLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            54242..54577
                     /gene="arnE"
                     /locus_tag="ACHKW0_000394"
     CDS             54242..54577
                     /gene="arnE"
                     /locus_tag="ACHKW0_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HFO7473285.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            54577..54963
                     /gene="arnF"
                     /locus_tag="ACHKW0_000395"
     CDS             54577..54963
                     /gene="arnF"
                     /locus_tag="ACHKW0_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HFO7473286.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPMTKQRY"
     gene            complement(54957..55223)
                     /gene="pmrD"
                     /locus_tag="ACHKW0_000396"
     CDS             complement(54957..55223)
                     /gene="pmrD"
                     /locus_tag="ACHKW0_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HFO7473287.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(55333..56688)
                     /gene="menE"
                     /locus_tag="ACHKW0_000397"
     CDS             complement(55333..56688)
                     /gene="menE"
                     /locus_tag="ACHKW0_000397"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008756 - o-succinylbenzoate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HFO7473288.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVMEYDHESVDLSEWVKDKLASFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(56685..57647)
                     /gene="menC"
                     /locus_tag="ACHKW0_000398"
     CDS             complement(56685..57647)
                     /gene="menC"
                     /locus_tag="ACHKW0_000398"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HFO7473289.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(57647..58504)
                     /gene="menB"
                     /locus_tag="ACHKW0_000399"
     CDS             complement(57647..58504)
                     /gene="menB"
                     /locus_tag="ACHKW0_000399"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HFO7473290.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(58519..59277)
                     /gene="menH"
                     /locus_tag="ACHKW0_000400"
     CDS             complement(58519..59277)
                     /gene="menH"
                     /locus_tag="ACHKW0_000400"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HFO7473291.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(59274..60944)
                     /gene="menD"
                     /locus_tag="ACHKW0_000401"
     CDS             complement(59274..60944)
                     /gene="menD"
                     /locus_tag="ACHKW0_000401"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HFO7473292.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTRDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(61033..62328)
                     /gene="menF"
                     /locus_tag="ACHKW0_000402"
     CDS             complement(61033..62328)
                     /gene="menF"
                     /locus_tag="ACHKW0_000402"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HFO7473293.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(62407..62712)
                     /gene="elaB"
                     /locus_tag="ACHKW0_000403"
     CDS             complement(62407..62712)
                     /gene="elaB"
                     /locus_tag="ACHKW0_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HFO7473294.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(62767..63228)
                     /gene="elaA"
                     /locus_tag="ACHKW0_000404"
     CDS             complement(62767..63228)
                     /gene="elaA"
                     /locus_tag="ACHKW0_000404"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7473295.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLDT
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            63293..64210
                     /gene="rbn"
                     /locus_tag="ACHKW0_000405"
     CDS             63293..64210
                     /gene="rbn"
                     /locus_tag="ACHKW0_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HFO7473296.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            64398..65618
                     /gene="elaD"
                     /locus_tag="ACHKW0_000406"
     CDS             64398..65618
                     /gene="elaD"
                     /locus_tag="ACHKW0_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase ElaD"
                     /protein_id="HFO7473297.1"
                     /translation="MMVTVVSNYCQLSQTQLSQTFAEKFTVTEELLQSLKKTALSGDE
                     ESIELLHNIALGYDEFGKKAEDILYHIVRNPTNETLSIIRLIKNACLKLYNLAHTATN
                     SHLKPTGPDNSDVLLFKKLFSPSKLMTIIGDEIPLISEKQSLSKVLLNDENNELSDDT
                     NFWDKNRQLTTDEIACYLQKIAANAKNTQVNYPTGLYVPYSTRTHLEDALNENIKSDP
                     SWPNEVQLFPINTGGHWILVSLQKIVNKKNNKLQIKCVIFNSLRALGHDKENSLTRVI
                     NSFNSELMGEMSNNNIKVHLNEPEIIFLHADLQQYLSQSCGAFVCMAAQEVIEQRESN
                     SDSAPYTLLKNYADRFKKYSAEEQYEIDFQHRLVNRNCYLDKYGDARINASYTQLEIK
                     HSQPKNRASGKRVS"
     gene            66007..66643
                     /locus_tag="ACHKW0_000407"
                     /pseudo
     CDS             66007..66643
                     /locus_tag="ACHKW0_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            66746..67249
                     /gene="yfbM"
                     /locus_tag="ACHKW0_000408"
     CDS             66746..67249
                     /gene="yfbM"
                     /locus_tag="ACHKW0_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HFO7473298.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLEA
                     LDRSELRKKFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(67316..68773)
                     /gene="nuoN"
                     /locus_tag="ACHKW0_000409"
     CDS             complement(67316..68773)
                     /gene="nuoN"
                     /locus_tag="ACHKW0_000409"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HFO7473299.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(68780..70309)
                     /gene="nuoM"
                     /locus_tag="ACHKW0_000410"
     CDS             complement(68780..70309)
                     /gene="nuoM"
                     /locus_tag="ACHKW0_000410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HFO7473300.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(70473..72314)
                     /gene="nuoL"
                     /locus_tag="ACHKW0_000411"
     CDS             complement(70473..72314)
                     /gene="nuoL"
                     /locus_tag="ACHKW0_000411"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HFO7473301.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(72311..72613)
                     /gene="nuoK"
                     /locus_tag="ACHKW0_000412"
     CDS             complement(72311..72613)
                     /gene="nuoK"
                     /locus_tag="ACHKW0_000412"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HFO7473302.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(72610..73164)
                     /gene="nuoJ"
                     /locus_tag="ACHKW0_000413"
     CDS             complement(72610..73164)
                     /gene="nuoJ"
                     /locus_tag="ACHKW0_000413"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HFO7473303.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(73176..73718)
                     /gene="nuoI"
                     /locus_tag="ACHKW0_000414"
     CDS             complement(73176..73718)
                     /gene="nuoI"
                     /locus_tag="ACHKW0_000414"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HFO7473304.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(73733..74710)
                     /gene="nuoH"
                     /locus_tag="ACHKW0_000415"
     CDS             complement(73733..74710)
                     /gene="nuoH"
                     /locus_tag="ACHKW0_000415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HFO7473305.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(74707..77433)
                     /gene="nuoG"
                     /locus_tag="ACHKW0_000416"
     CDS             complement(74707..77433)
                     /gene="nuoG"
                     /locus_tag="ACHKW0_000416"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HFO7473306.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSLE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(77486..78823)
                     /gene="nuoF"
                     /locus_tag="ACHKW0_000417"
     CDS             complement(77486..78823)
                     /gene="nuoF"
                     /locus_tag="ACHKW0_000417"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HFO7473307.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGAWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(78820..79320)
                     /gene="nuoE"
                     /locus_tag="ACHKW0_000418"
     CDS             complement(78820..79320)
                     /gene="nuoE"
                     /locus_tag="ACHKW0_000418"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HFO7473308.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(79323..81125)
                     /gene="nuoC"
                     /locus_tag="ACHKW0_000419"
     CDS             complement(79323..81125)
                     /gene="nuoC"
                     /locus_tag="ACHKW0_000419"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030964 - NADH dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH:ubiquinone reductase
                     (non-electrogenic) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HFO7473309.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(81219..81881)
                     /gene="nuoB"
                     /locus_tag="ACHKW0_000420"
     CDS             complement(81219..81881)
                     /gene="nuoB"
                     /locus_tag="ACHKW0_000420"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HFO7473310.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(81897..82340)
                     /gene="nuoA"
                     /locus_tag="ACHKW0_000421"
     CDS             complement(81897..82340)
                     /gene="nuoA"
                     /locus_tag="ACHKW0_000421"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HFO7473311.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
BASE COUNT        20202 a        22371 c        20478 g        19395 t
ORIGIN      
        1 ctggcggcag cagtccgcca ggtcagcgtc gtaatattat gccgtccggg caacggcatc
       61 acgcgaagca gcatcgcgct cttcgcccgt cagctcgcta agttgcccgt tgcgcatttc
      121 cagcaggcgg tcggcgtgga taaagtaatg atcatcatga ctgatggcga aaatagtttt
      181 acccatctcc tgcatcagcg gcagcaacac ctgataaaac tcacgacgga agtgtggatc
      241 ctgatccgcc gcccattcat ccagcaggat aatatcgcgt tcttctgcca gcgccagcaa
      301 cagcgccacg cgttttttct gcccttttga taacttcagg ttaacaatac gcccgttgct
      361 taactcaaac ttatgagcca ttttcagctg cgccagccac ttctcaacca gttgcgggtt
      421 agcgggttta ccctccggcc ccagcagttg atcaaacagc cagacatcgg taaacactgc
      481 cgaaaacagt ttgcgataat cttccggttg ttcgccgctg acaggtttgc catccagcaa
      541 gatttcgccg ctttgtggtt gatacaagcc cgtcaacaac atcgccagcg tcgattttcc
      601 gctaccgttg ccgccaatca gaaacagcag ctcgccacgt ttgatggtga gattaatcgg
      661 accaacggaa aacgcgttat cctgataagc aaacgtcacg ttacgcagct ccagcgtttg
      721 ccagttggga aacgcctgcg ggcgcggaaa ctctgctttg aaaggcgcga gcgcgaattt
      781 gttcagcttg ttaaacgcca cctgcgccgt cagcagcgtc ggcaatgcgc caactgccga
      841 aagcagcggc gtacgcagga ataaaagcgt caacgaatag gtcgcggcaa cgttggtatc
      901 agcccaaccg agactgttcg ccatccagaa caccaggccg attgcgccca acatcatgat
      961 gtttgaccag ttcacggcgc taagatggaa ggtgtctgcg cggataatat ggtggcgata
     1021 ctcttgcgca tcaggaatgt agaggttgtt aaacacatac tcggcgcgtt cccggttcag
     1081 agtcagctct ttgcgccctt cgagtacagt ttgaaaatcc gtatacagcc tgtcttcggt
     1141 ttcacgcagg gtcgccatat gtttgtacac ccgcgccacc agtacaaaac caccccagat
     1201 ggtgatcgcc atccagatag ccgttaccag caacattttg cccgacagca tccacagata
     1261 cgccgcggaa ccgatagtga gaatgatccc ctgcaccagt tccggaagac gcacaaaagc
     1321 aatggtgata ttgcgcacat cgctggttaa tcccgccagc aacgaggcgc taccgagttg
     1381 ttcaatgcgc tcgacgttgg tatccagaat ccgcttgata aattcactac gcagtcggta
     1441 aacgaagtga tgccccaaag tggtgagcgc cagttgcgat ccgagagtga ctgccatcaa
     1501 cagcagcaat aatcccagaa actccggcaa taccagcaga ctggtatccg ccgtttcgat
     1561 aaggcgctga ttgataaaag caattaagcc aatgcctaat gccgcactgg cgaggcttag
     1621 cgccatcaca ctgataaatg gccagcgata ctgccgccag acaagtacaa gaagttccat
     1681 gcagaaaacc cggacaatga attacagccc gcagtttaaa catcttcgcg cgcacagcaa
     1741 taataattct tatttttatt cttttttacc tgcctgacgg aatgtcaggt tgtagcggca
     1801 gtcggtggtg agtggatgaa accccgcttt caacggttga ataccgtgat aaaacagccg
     1861 cgactcaccg ccccacacca ccacatcgcc atgttccaac aacaaacgtt tgagcggatc
     1921 attacgtttc aggccaccaa attgaaaaat cgcaggtaag cccagagaaa cagaaacaat
     1981 tggcgcgcgc agatccggtt cgtctttatc ctgatgcagc gacagtttcg cgccaggagc
     2041 gtagcggttg ataagacaag catctggctg gaaatctgga tagcccgccg ccgtagccgc
     2101 acgttgacat aaattatgaa aactctgtgg catggcgggc cacggtttat ttgtttgcgg
     2161 atcaatgggc gaatagagat aaccttgccg atggctcgtc cagcccagat gcccacagtt
     2221 ggtcatcgcc accgacatgg tatatccccc gggggtgacc atctggcgaa acggcgactg
     2281 gctggcaacg tcattaatat cgcggattag ttgctccgca gcattaaaag caaaacgccg
     2341 taaaattacc gcaccagccg ccagtggctc ttgccacggt tcagcatcgg caaacagatc
     2401 caacattacc tctcctcatt ttcagcttcg cggcgcagca gttgcgcttt acgcgacacg
     2461 ccccagcggt aaccggaaag tgtgccatca ccacggacca cccgatgaca gggtattatg
     2521 atagccagct tgttggcggc acaggcgctg gcaacggccc gtaccgcttt cggtttgccg
     2581 atggcgttag ccagttgctg ataactgacg gtttcaccgc aaggtatcgt gcgcagtgcc
     2641 tgccagactt gttgctgaaa agcagtgccg cgaatgtcca gcggtaacgt cagcggcgta
     2701 tcgcgttgat tgaggctggc gatcacttca cgcacatgtt gctgaaacat cagatcggca
     2761 ggcgcgttgt cggcagcggg aaacatctgc tgcaactcgc tgattagtgt cgcgtcatca
     2821 tcgcccagca atatcgcgca aatcccccgc tcgctttctg ccaccaggct acgacccagc
     2881 tcacaatcag ccagcgcgta acgcaccgcc agattttcgc caccgtgacg gaattgttta
     2941 gccgtcatgc ccagcgtttc atcggctttg cggtagtaac tgctgctgtc ggggaatccg
     3001 gcgttaagaa tagacgtcgt cacgctctcc cctttcgcca gcgattcgcg caaacggcga
     3061 gcgcgccagg cctgttgcca ggctttaggc gtcattccgg tagtcgcttt aaacaaccga
     3121 tgtagatgaa atggactcat caccacctgg tcggctaagg cttccagcgt tacaggcgtt
     3181 tcctgttcca gcagtcgaca cgcgtgggtg attttatcca accgatgttg ctgggcattg
     3241 gctttttctg gctgacaacg tttgcagggg cgaaagccag cggcgagtgc ctcgctggca
     3301 tttgcgtaga aggagacgtt ttcccgcaaa gcatgtctgg cgcggcaaga cggacggcaa
     3361 aagatgcctg tggtacgcac ggcgaaaacg aattcgccgt cggcattcgg gtcgcgggct
     3421 aagacagatt gccagcattg atcgtcagtt aagcatgtgg cttttttcat aatcagctcc
     3481 ctggttaagg atagccttta gtctgcccgg tcaccatcac gcaaaaacca acaatcttgc
     3541 gctttaattt ttttcgctga taaggaagct tttaaactgc ggcgacatcc aggttttaaa
     3601 gctatcacct tctttggtga tcatatagac cgccagccct tcccggcgaa caacctcttt
     3661 ggctttctcc ggcccgagta ccattaagcc agtatcccag gcatcggctt ccagcgccgt
     3721 cggggcaatc accgtcacgg ataccagatt gtgttcgata ggacgcccgg tttgcggatc
     3781 gataacatga gaaagacgtt tgccgtccag ttcgtaatag ttacggtagc tgccagaggt
     3841 gctaatgcca tgaccgttga tatccaccac ggcctgaacc gcgttttctt tatcggttgg
     3901 tttttgaatc gctacccgcc acggctggcc ttcaccgttc ataccacggc tatccagagc
     3961 accaccaact gacaccagat aacgtgaaat accttcctgc tccatcaagc gtgccaggtg
     4021 atccgccgca taaccttcac cgacggtgga gagatcgaca tataaatccg gcaggtcttt
     4081 ttgcagatat tgctgatgcg actgattgat gaccgtcagg tgctgtaagc cggttttggc
     4141 tttcatcgca tcgatctgtt cctggctcgg aatttgaacc ggctgttgtt ccgggccaaa
     4201 gccccacaga ttcaccagcg gcccgacggt tatatccatc gcaccatcgg tccttgcgcc
     4261 aatgcgcagc gaggtggtga cgatatcggc catcgcttca cttaccggcc acggcgacaa
     4321 actttgcgag tcgttaaagc gcatcagcgt ggaatctttt ttataggtcg aaagcagctg
     4381 atcgtcggcg tccagctggg tctgaatctt ttctttaagt tcggcactgc gtttggcatc
     4441 aatgcccggg atgctggcac gccagaaggt acccatggtt ttgccttcaa gaacggtaac
     4501 ttcagtagcg tgggttttgg cgggttgtgg tttttgatcg caaccaacaa agaagagcgc
     4561 ggcagccagc agtgccacgc gggtaaagct tatttccata cgtgattatc ctcatgcgaa
     4621 cggtcgcaag agtacaccaa aaaaatgagt ttgtacgctg aaaacaatga aaaaagggcc
     4681 cgcaggccct ttgttcgata tcaatcgaga ttagaactgg taaaccagac ccagagctac
     4741 gatgttatca gtgttgatgc cagcgtcacg agtgaactgg ttgtcgtcca gcaggttgat
     4801 tttgtagtca acgtaggtgg acatgttttt gttgaagtag taggtagcac caacatcaac
     4861 atatttcagg atatcttcgt cgtcgtagtt acgaccagcg atggtaccca ggtttttacc
     4921 tttagactgc aggtatgcta cagacggacg cagaccgaag tcgaactggt actgagcaac
     4981 agcttcaaag ttctgtgctt tgttcgccca acccagggaa cctacgcgag ttgcgttata
     5041 ggtctgggtg tactgagcag ccaggtagat gttgttagcg tcgtatttca gaccaccggt
     5101 gtaggtttca gcacggtcgc cgttaccgat gtacagcggg ctgttctgat catcagtacg
     5161 tttggagctg gagatcgcac caccgatacc gaagccttcg taatcataag tgatagatcc
     5221 gccaacgccg tcgccgttct gacgcagagc accacgaccg ttgttggtca tgccttcgcc
     5281 gcttacgcta ccgtttttac cctggtactg aacagcaaag ttcaggccgt caaccagacc
     5341 gaagaagtca gtgttacggt aggtcgcgaa gccgttacca cgctgctgca tgaagttgtc
     5401 agaaccgtag gtgtcaccac cgaattctgg cagtacgtcg gtccaggaag ttacgtcgta
     5461 aacaacgccg tagttacgac cgtagtcgaa agaacctaca tcctggaatt tcagacctgc
     5521 gaatgccaca cgggtccagg agttgttttc gttttcagcg ctgttgccct gaatctgata
     5581 ttcccactgg ccgtaaccgg tcagctggtc agtaacctga gtttcacctt tgaagccaag
     5641 acgcatgtag gtctggtcgc catctacatc tttgttgtca gagaaatagt gcaggccgtc
     5701 tactttaccg tacagatcta atttgttgcc gtctttgttg taaacttcag cagcgtttgc
     5761 tgcgcctgct accagcagag ctgggaccag gagggacagt actttaactt tcatgttatt
     5821 aaccctctgt tatatgcctt tatttgcttt tttatgccac tgcatactga ttaaccctca
     5881 ttaatcagtc ggcaagtcca ttctccccaa aaatgcagaa taatccaaca cgaatatgat
     5941 actaaaactt ttaagatgtt tcatttatcg ctatagatgt ttcaaaatgt aaatgcaagg
     6001 gaacttttta agattattgc ggaatggcga aataagcacg taacatcaag caataataat
     6061 tcaaggttaa aatcaataac ttattcttaa gtatttgaca gcactgaatg tcaaaacaaa
     6121 accttcactc gcaactagaa taactcccgc tatcatcatt aactttattt attaccgtca
     6181 ttcatttctg aatgtctgtt tacccctatt tcaaccggat gcctcgcatc cgtttttttt
     6241 acccttcttt acacactttt cattattctg tgctaccaca gaaaaactat aacgcttgtt
     6301 aactatttca caaataatta acatcagcat aatttccagc aatctttgtt tatttgcaat
     6361 tatttttgtt gggctttttg taggttattt gtacagcaaa atggcgcttg tacatctatt
     6421 tcccccaatg caggatgata aatatcacgg gagaatagag aatcatcaat caggtaagag
     6481 tctggaattt cacactgtac cctttatact gccctatcac ttcgcgaagt tttaacaggt
     6541 cataaacacg aatgcgtcag aaagagacaa cggccacgac ccgcttttca ctcctaccgg
     6601 ggagcattac ccgcttcttt ttactgttga tcattgtgtt actggtgacg atgggggtaa
     6661 tggtacaaag cgccgttaac gcctggctga aagataaaag ttaccagatt gtcgacatta
     6721 cccacgctat ccaaaagcgc gtcgatacct ggcgttacgt gacctggcag atctacgaca
     6781 acattgccgc gacgacctcc ccctcctccg gcgaaggttt acaagagacg cgcctgaaac
     6841 aggatgtcta ctatctggaa aaaccgcgcc gcaaaacgga agcgttaatc tttggctctc
     6901 acgacaactc aacgcttgag atgactcaac ggatgtccac ctatctggac acattgtggg
     6961 gcgcagaaaa tgtaccgtgg tcgatgtatt acctgaatgg tcaggataac agtctggtgc
     7021 tgatctcaac cctgcccctc aaagatctca cctccggatt taaagaatcg accgtcagtg
     7081 acattgttga ttcacgtcgt gcagaaatgt tgcaacaggc caacgccctc gatgaacgcg
     7141 aaagtttttc taacatgcgc cgcctggcct ggcagaacgg tcattacttt accttgcgta
     7201 ccacgttcaa ccagccggga catctggcaa cggtcgtggc ttttgatctg ccgattaatg
     7261 atttgatccc accgggtatg ccgctggaca gtttccgcct tgagccagac gcgacggcaa
     7321 cgggagacaa tgataatgag aaagaaggga cggatagcgt cagtatccac tttaacagta
     7381 cgaagattga aatctcctcg gcactcaact ctaccgatat gcgcctggtc tggcaggttc
     7441 cttatggcac cttattgctg gatacgttgc aaaacattct gctgccactg ctgctgaaca
     7501 tcggtttgct ggcgctggcg ttatttggct ataccacatt ccgccatttc tccagccgca
     7561 gtacagaaag tctacccagc acggcggtca ataacgaatt gcgcatttta cgggcaatta
     7621 atgaagagat agtctcactg ctgccgctcg gcctgctggt tcacgatcag gaatcgaacc
     7681 gcactgtcat aagtaacaaa attgccgatc atttgctgcc gcatttgaat ctgcaaaaca
     7741 tcaccaccat ggcggaacag catcagggga ttattcaggc gacgatcaat aacgagctgt
     7801 atgagatccg catgttccgc agccaggtcg cgccgcgcac acaaattttc attattcgcg
     7861 atcaggatcg cgaagtgctg gtaaacaaga aactcaagca ggcgcagcgt ctgtatgaga
     7921 aaaaccagca ggggcggatg acctttatga aaaacattgg cgatgcgctg aaagaacccg
     7981 cacagtccct ggcggagagc gcggctaaac tcaacgcccc ggaaagcaaa caactggcga
     8041 atcaggcaga tgtgctggtg cggctggttg atgaaataca gttagcgaac atgcttgcgg
     8101 atgatagctg gaaaagtgag acggtgctgt tctccgtgca ggatttaatt gatgaagttg
     8161 tgccttcagt gttgcctgcc atcaagcgta aaggtctgca actgctgatt aacaatcatc
     8221 tgaaagcaca cgatatgcgc cgcggcgatc gcgatgcctt acgacgtatt ttgctgctac
     8281 tgatgcaata tgccgtgacc tcaacgcaat tgggaaaaat cacccttgag gttgatcagg
     8341 atgagtcctc cgaagaccgc ctgacgttcc gcattctgga cacgggagaa ggcgtaagta
     8401 ttcatgaaat ggataatttg cacttcccgt ttatcaacca gacccaaaac gatcgctatg
     8461 gcaaggcgga cccgctggca ttctggctga gcgatcaact ggcacgtaaa ctgggcggtc
     8521 atttaaacat caaaacgcgg gatgggcttg gtacacgcta ctctgtgcat atcaaaatgc
     8581 tcgcagctga cccggaagtt gaagaggaag aagagcgttt actggatgat gtctgcgtaa
     8641 tggtggatgt tacttcggca gaaattcgga atattgtcac tcgccagtta gaaaattggg
     8701 gtgcaacctg tatcacaccc gatgaaagat taattagtca agattatgat atctttttaa
     8761 cggataatcc gtctaatctt actgcctctg gcttgctttt aagcgatgat gagtctggcg
     8821 tacgggaaat tgggcctggt caattgtgcg tcaacttcaa tatgagcaac gctatgcagg
     8881 aagcggtctt acaattaatt gaagtgcaac tggcgcagga agaggtgaca gaatcgcctc
     8941 tgggcggaga tgaaaatgcg caactccatg ccagcggcta ttatgcgctc tttgtagaca
     9001 cagtaccgga tgatgttaag aggctgtata ctgaagcagc aaccagtgac tttgctgcgt
     9061 tagcccaaac ggctcatcgt cttaaaggcg tatttgccat gctaaatctg gtacccggca
     9121 agcagttatg tgaaacgctg gaacatctga ttcgtgagaa ggatgttcca ggaatagaaa
     9181 aatacatcag cgacattgac agttatgtca agagcttgct gtagcaaggt agcctattac
     9241 atgaacaata tgaacgtaat tattgccgat gaccatccga tagtcttgtt cggtattcgc
     9301 aaatcacttg agcaaattga gtgggtgaat gttgtcggcg aatttgaaga ctctacagca
     9361 ctgatcaaca acctgccgaa actggatgcg catgtgttga ttaccgatct ctccatgcct
     9421 ggcgataagt acggcgatgg cattacctta atcaagtaca tcaagcgcca tttcccaagc
     9481 ctgtcgatca ttgttctgac catgaacaac aacccggcga ttcttagtgc ggtattggat
     9541 ctggatatcg aagggatcgt gctgaaacaa ggtgcaccga ccgatctgcc gaaagctctc
     9601 gccgcgctgc agaaagggaa gaaatttacc ccggaaagcg tttctcgcct gttggaaaaa
     9661 atcagtgctg gtggttacgg tgacaagcgt ctctcgccaa aagagagtga agttctgcgc
     9721 ctgtttgcgg aaggcttcct ggtgaccgag atcgctaaaa agctgaaccg cagtattaaa
     9781 accatcagta gccagaagaa atctgcgatg atgaagctgg gtgtcgagaa cgatatcgcc
     9841 ctgctgaatt atctctcttc tgtgacctta agtccggcag ataaagacta atcatctgta
     9901 ggccagataa gacgcgttag tgtcttatct ggcatttgca ccgattgccg gatgcagcgt
     9961 aaacgcctta tccggcctac gattcccatt atttcaacag attacattaa agtaggccag
    10021 ataagacgcg tcagcgtcgc atctggcatt tgcactgaat gccagatgcg gcataaacac
    10081 cttatccgtc ctacgattcc cgcgatttcc tgaccctctc ggcatatacc gtcagcgtct
    10141 gttttatcac atccagcgtt accggcttcg acaggcagct gtccataccg gactccagac
    10201 accgctgctt ctcttcagcc aacgcattag cagttactcc gattaccggc aacgtcagtc
    10261 ccaactgacg aatgcgttgc gtcaagcggt aaccatccat atttggcatg ttgacgtcgc
    10321 taagcacgat atcaatatga ttcttgctaa gtacattaag cgcatcgaca ccatcattcg
    10381 cggttttaca ttgatagccc aacgatccca actgatctgc cagcagacgt cggttaatcg
    10441 gatgatcatc cacgaccaga atcatcatat cgtcattatc gctgaccgct ttgtccgtcg
    10501 acggcagagc gttagcagga tcgtcgctct ccatctcgat caaataaata cgcgccaaca
    10561 atgccggtag ctcatgcggg gcagccacac tgtgtaccca ctcccctggc gctttctcca
    10621 gcggaatacc aatatggcga cgacagaagg tcactaccgc tctaccctgc cattttttac
    10681 tcactacctc gtcagtaatt aacacatctt cgggagtcgg ttcctgccct tcgtatgttg
    10741 taacgacgat gccgctgcgc tgcaaactgg tttccaggaa ctgacagagc gacgcattgc
    10801 ggaccgccag ccagcagcgt ttaccactca acccttccac gccttttttc tgcgggtact
    10861 gagcgccgta caacggaata cgcacggtaa actggctgcc cattcccggt tctgaatcta
    10921 ccgagatatc gccgtccatc atgctgatca gtttttcaca aatcgccaga cccagaccgg
    10981 tcccctggaa gtttcgctgt acgcccgttc cgacctggaa gaagggatca aacaagcgca
    11041 ccacttcttt cgccggaatc cccacgccgg tatcgcgaac acggatagag agataatcgc
    11101 catcagcgcg gacatgcaaa actatacagc cggtatcggt gaattttatg gcgttactca
    11161 acaggttgga gatgacctgc tgtaaacgca tcgggtcgcc atttaaggcc actggcacat
    11221 ccggttcaat aaagcagtac aagcctaact gcttgcgtac caccagcggt aaatagttgg
    11281 cggtgatgtg gttcatcact tcacgcggtg aaaactcacg cggttcgatc ttcaactgtt
    11341 ccgattcaat cttcgagaaa tcgagaatat cgctgataat tttcaacaac aggctggaag
    11401 agttgttcat tgccgtcacc agccgatcga cgcctttcgg taactctttg gtttgcaaca
    11461 gatccaggtt accgataatg ccatacagcg gcgttcgcag ctcatgactg acggtggcaa
    11521 ggaacatcga ttttgactgg ctcgcctgtt ccgctgcttg tgccatctcc tgcaacgact
    11581 cttccatctt cacgcgcgaa gaaacatcca ccagcacaca aatggccacg ttttcattac
    11641 gatagcgcga atggacgaag ctgatttgca gattggtatt gttgctggtc aggacatcaa
    11701 caaaattgac ctgctgccca cagataattt gcgtcagtcg ttggcggtcc tcatgcgtaa
    11761 gcatattgag ataggtatgc gccagttcgt tacttaaaat attgacgcca tcagcggtac
    11821 gcaaaataca gatacccact ggcgcggagg cgacaatctt gcgattgaac tgctcatgtt
    11881 cttccagtcg cagggcgtcg ctttccgccg gaatgaaaat acgtcgctcg tacatccgcg
    11941 cgagagtaaa caatgcagct ccggcaagca cattcagcaa aattgcgtta aggatcaaca
    12001 tgcgaatgcg ttccagcacc ttatcaaccg gcaccgaata cacgatgctt agcgatgagg
    12061 gtggcagatt tttcttcagc accagctccc ggaacccttc cgtatagcca aaccaggagc
    12121 gttcctgcat ccagcgagga tcgcccttaa ttttactttc tggtccggta agcgaaatca
    12181 gggtatgacc attttcatca agaatggtaa cccccatcgg caacgtaccc ggtaagaaaa
    12241 agttctccat ccggatggtc tgctcgacac ccaaaagcgc ctgcaaccgg ttcgccagat
    12301 aaactggcgt caacgcgtaa aaatacccga cgccagggcg cggaccttcg ctgatccagt
    12361 agaggttact gccgctatca tcttgtggtg catttcgata tttattgatg cgttcatgca
    12421 aagctttcaa cgcggtatcg cgttccactg gcatatcacg cagaccgaaa ttggccatgc
    12481 agaggttatc actgccgatt aaaaataccc ggttgagatc gtaagccgca gaaaaattat
    12541 cgcgccagta gcgcataaac cacgccaatg actccagaga acctcgccag gtgttactca
    12601 ttgcggaaca atcggagtcg gcaaacagcg gttcaaacgc aggcacatcc gcctgcgttt
    12661 ctcgtccacg cggggaaagc acaccgtttt ctgccgataa gcgattttcg gcgatgtact
    12721 tcagctcttt catcacatca gaggtgcgtt gaataaagcg ctgagcctga tcggaactca
    12781 gattaaattc ctgacgaatt tccgattctc gctgatgtaa cgcattaacg atgtaaaaaa
    12841 cggatgaaaa agcaatcaac agccagagca ctaacgccaa tgctctgaac atgtagcgcg
    12901 aggctttcag ggttgtacga aaagaagcaa ggtatttcaa aggggcgaag ctccgcctca
    12961 ggtgaccgat ggagtgtggt taaggtagcg gtaaaagcgt gttaccgcaa tgttctgtca
    13021 ccattgcaat aatgcgtatc gcttagaaac cattgcctta accaggaaag ccggagtaga
    13081 tggacagttc tcagtacgaa aaataagaaa tgaccaatct gccgacgctt gttaatggtt
    13141 atatcctggc gtcggcagat cgcccagcag tcttatctga aaaaagagag agccaggttg
    13201 tgtgaaggga tggatcacca gtcaaaactt ttcaccagcg tcagctcgcc agcattacgc
    13261 atcggtacaa taaatgtttc ctgtttctca ttgaccgatc cttcatcggt gatcagcgtc
    13321 agttgggcgg tcgtcaattc cgtttcgctg cgcccaccat agtagttgat atacacctga
    13381 tagcgcccgt gaactggtgc tggcatggcg aaaatctcgg gtccgtaccc cgtcgtgaca
    13441 tccatatcca gtgcaccact gtttttcagc acggtgttac cgtaccaggc gtgttcgcca
    13501 tcgggcgtga caacatggag atcgagatcg gtattgtccg tatcccacga gagaaccagc
    13561 cgtaaacgtg cacgaatcgt tcctgtaccc ggcgttgagt aaaactgcat tttttgtcgg
    13621 ctttgcccat ccgggctgat gacctgcacg ctgttgctgc cttcagtgaa aatataaggg
    13681 cgtgcaaatg aaccatcgga ttcaactctc tgcggcatac tggcaccgtt gaccaccagc
    13741 cggccctgct gaactttacc cgccgcagca taattcttta ttttgccgcg gatctgcgct
    13801 gagatatttt gatcatctgc catgttgacg gacgacgccg ggtaattgat cgattgtgaa
    13861 aaactggcat cttcgccctc tgccggatgc cagccagaga gcggtgcgtc aatctcgact
    13921 tcctgcacac cttcactgtg agcgacaggc gaaagtgcca ccagtaacaa cggaagaaaa
    13981 atctttcgca tagcattagt ccattaataa ttgtcgggtc agcgtttcga tatagttctc
    14041 atcaagcccg gtgggataac catcaaaggc caggtgcaga tactcatggg ttaaatccag
    14101 ccgatcctga agcgtaaaga agttacgaat aaacagccgt ttctgctgcc gatcggtata
    14161 ggggaaacct gagactaaac gacagaccgc aaacacgtct ggttcgttgt accccgtctc
    14221 gccttgtaat atacgccgcc actgcggcat ttttttcgcc agccaggctt tcgctttggg
    14281 taataactgg caggttgagc gtggagctcc ccagcggcta aggctgttgt cgggataagc
    14341 aaacgcgagt atctggtcgt aacgctcccc ctgccccgcc tgggccgtgg cttgccgcca
    14401 ggaaagtgtc ccttcggtag cgcgactgcc atgataatga accggatcgc ctgcatagat
    14461 gagatcctgc gtccaggcgg tcatcgttcg cgcccccgtg gtcgctggcg aagcagaaac
    14521 acgctgcgtg gcgctgctgt cggggatggt caggcaatcg ccttcccgat tggcgttttg
    14581 ctgtaaaaaa gtgcgaatcg ccacggtcat ggctttcgct gcctcagggg gcgtactttt
    14641 cgcctcgcga tccagaactc tggcaacgta ctcttcacga tcgagatgcg actgcaattt
    14701 cagtttgccc ttttcgctca gtaaggtcgt ctcaccgtgg ctgacaaacg taatgtggtt
    14761 accattagta aacgtcacac gatagcgccc attaagtacg ccgggtttta ctgccgtcgt
    14821 actcttttcc gctgtgattt tcttcagcgg atagcgggca aacagctcca cctctacaca
    14881 ttgcccactg gcaacctgcg tcggcactgg caaaacccga ttaagcaccg tcgcataacg
    14941 ggttagtacc gttttgctgg tgccactgcc agtaacccac agcggtgtac catcggttaa
    15001 ccagccagca aaaccaccct ggcggatttc ctgtttatca tcggcaaacc agctccaggt
    15061 tttcacccgt acccgactac cgagcatgga tgcgacaccg attttcgcct cgtccagcac
    15121 cacatccagt aagacttcct gagctttgtt ttgcgcaggt aacgtcgcta atgaatcgag
    15181 cagcgattta accgttaccg aggtttctgg cttcagcgtt gtaagagacg ctaaccacgc
    15241 aggtgcctga cgctgttgcc agtattgccc ccacacatct gcgccaatat gcaaccgctg
    15301 cggcgcaaaa tagagcccac aggaacgtac cagcgcggta tcgcgcgtta tgctctcccc
    15361 agcctgacaa caataaacct cttccgggga gtttccgcga cactggtagg gctgctccgg
    15421 ttgatgggta tcctccagcc aggcgtaaac atacagtttc cacaaactcc ctaacggcgt
    15481 ggtgagcgag tcaggtaatg ctgagacttt ggtcacgcct gatgacgaca gctgatacag
    15541 ttgatcgtgc tgcgcaccgc gcagcaccag ttgtaacggc gtctcctccg caagcgttgg
    15601 cagagtaacc aacgccaata gccagacgat tctgcgccag ttcacttatt tcacctgcat
    15661 cccggtccat tcactccctg ccgcaacact ttgctgcgca ggtgcatagg aacgcacata
    15721 acgagcagga ggcagaacga attgcccttt ttgcgagaag cgcagcaaat ggcggaaagt
    15781 gaccgttccc gtcagttctt tcaccggcac catataggcc agttcgccca tttcattacg
    15841 cgctttttcc agcaattgcc cctgctgttt cgcggcgttg ggtttattga ctgaaatgcc
    15901 ccatgttgtg cgctcaacgt cggctcccgg cgggagcggt acttccacct gaccatagcg
    15961 cagaactgca tcctgctcgc tggtaagcgt aatttcatcg agatacagcg catcgctgtc
    16021 aatctcattg ctggtcaccg gctgcagagt aaagctcatc tcttcttcac cggggataag
    16081 ccgatacaac tggcgttcaa cggtcaccgg aatgttactt tgttgagccg tttttgccgg
    16141 ttcacgccag cggacctgca cattttgcgg cgataattcg tcaccaaaag agagaatgtc
    16201 cggcacaccc tgaccaaccc aacgccagtc ttcgccccct ccagttaact tatgtttagc
    16261 ccatgcgccc gcaggcgcag gcaaaacaac tggaggcatt gtcgccatat atttcgccag
    16321 ccagttcatg gccagcgcgc gctcaatagt ggattgctca gcggtcaaac cacttaaaat
    16381 agcggccgtc tgggtagcgt caccaccgct attgagcagc acaaccgtgt gcgccagcgg
    16441 ctgattgctg tttgccgctt tatcccacgc atattgctga acctgtggca tcaccgtgga
    16501 cttcaactgc gcttttttca gcaacgtcat ggttaagacg tttgccaccg catctgccag
    16561 tggagattcc ggtgtatcga ggatcaggct gtcattgata tcgcggatgt cttcctcaga
    16621 gaaatcttca tctttagttc cgcgccgggc gatagcttca tccaaccctt tcaacaacgt
    16681 tttgcacggc agattcatct cctgtgccca cgccagcacc agcgcccgat gcaataacgg
    16741 catgttatcc gcctgctcgg cataactgtc gagcatatgc tgccagtatt ccggttgttg
    16801 cgttacgccg agcgcctggc tggcctgcca gtcggcgtac catgcccatg ccgtaaggaa
    16861 ggcgtcacca ttgccatctt caccccacca ggtaaagcgc gctccgggcc ccgccagttg
    16921 catcagccgc agacggttat cctgaatcat ctgacgaatg tcgttagcgg cggcactttg
    16981 atgatcggca agcgaacgcc aggcgagact gagcgggatc agacggctac cggtgttgat
    17041 cacgccaccc catggtccat cgactaacgc atcaagattg ttgcggaaaa tctcctgcgg
    17101 cgtttcgcta ctttgtagcc ggatattgct agcctgctcg ggcaacatca gcgcgttctc
    17161 gccaccgccg agcatgacat ttttctgctg ttcaacgggc cagctgttat ccacaaaaga
    17221 cagttttgtg ctaatgctgt cctgcacttg cccattttgt tgcagttctg cgcttaacaa
    17281 gccagattgc tgaatgttct gcgccagcga aatataattc gcccctttgt gcagcgtcag
    17341 cgtctggcgc atctcagcgc ctgcaaattt agtcaccagc gccaccggtt cgttatcctg
    17401 ctgactgaag ataaacagtc ctgccgccgg tttgtcgccc acgcgataca ccgttggcat
    17461 actccacttc atgtagagat ttttttccga acgcagataa gcacgcccct gcccgaccag
    17521 cccgtcgccg ttcatcccac gcgcggtgat acgccagcgg gttaacgaat caggcatcag
    17581 gaacgtgaaa tacgctttgc cttgtttatc ggttgtgagt gacggcatcc atgccgcggt
    17641 atccacctct tcacgccgtg gacgttcaag catttttact cgccgctcgc tgcggttagt
    17701 tgcgccaggc gcaaccggct cgctggagag cgcctggtcg tagctgataa acgacagact
    17761 ggagctggta cgcacattgt tacgccccag cggatagaaa aatttgccga tattcggcgc
    17821 gatttctggt tgcagcgcgt agatcatttc atcgaccacg cctaccgtta gctgcgcaga
    17881 aacaggttta cctttcagcg acgaggttaa ttcgacattg accagttcac caggctggta
    17941 atgggttttg tccgttttca cccggatatc aagctgagga acggcaactt tgatcccggc
    18001 gttctgaaaa ctgtactgac cattacgggt atacagcacc gaaaaagtga tgttaggcgc
    18061 aaaggaattg ctcactggaa cccgggcttc atactgggta tcgtttaaac gttgtaacgt
    18121 cagccagttt gccggatgcg aaagcagcga ctgctgttcc acgcgatcgc gttccagcgt
    18181 caataatgct tcatcaattg gctccggaaa ggtaatcagc atcttcgcag tttcgcctgg
    18241 ctggtacagc gttttatccg ccacgatatc taccgtacca gtatgcgccg tgctgccctt
    18301 accgctgacg gcatgactta acccagcgag aattaagccg tctttatcgc gtaatgtcag
    18361 attgtagttg ccaggtttag cgaaattgac ggtaaaggat ttgccgcctg acggtagctc
    18421 tccgctatgg ctcgtgcggt cttcgagacg caaccattca tacgtaacag gaacctgttt
    18481 tgaagattcc agcgcggcat aacggaacac aaccgactcg ccgctattac tgtattgtgc
    18541 ggcagtactt aatgagtaat gcgccagacc gcgttcaatg aggatctctt tggtggtggt
    18601 gacacgatac gccgcgccgt cactggcgga gacggttaac aaataacggc tcggtttatc
    18661 ggcggcgggg agatttaacg ccacatgacc gctggcgtcg gacaccgttt cgctgccttc
    18721 cagcgacacg gggaaacgtc cggcataacg caaatcgtta ccgaccattg ataattgctg
    18781 agcgcgcaaa cttaactgca cgcgggcatt ttttaccggc tcgccatccg ggtagagcag
    18841 ttgcagtttg ccgctgaccg cttcgccagt tttgaactct tttttgtcga gagctaaacc
    18901 aatctcgaaa tgtggcttga tgtagtttgc cacgcgaaaa ctgctgctat agacctgatt
    18961 gcggtaagca agacgtaact cataacctcc ggcaacggca ttttctggca ggcggaaact
    19021 tccctgcccg ccattgcgcg catccagcgt gacatcgacg gtttgcaaca gactgccgtt
    19081 agcgtccagc accgaaagct tcgccggggc gctgacgatg ggggatgaat gcaacggatc
    19141 gtggaactcg cggcccatca ctttaacatc gacacgatcg cctgcgcgat atagcgggcg
    19201 atcggtaaaa atatacaagc gggtgttgta gatttcgctt tcgtagaaga agttctcgga
    19261 gacaaaaaca ccgccttcag catccttacc cagaatgtat gaacgttctg gcgatatatg
    19321 ttgtaactgc aaggtaccgc tgtcatcggt cacaccgcgg gtcatcacgc caagaccgtc
    19381 agtccacaag atctctgagc cgggcttcgc ttcaccctgt tttttacccg cggtccacac
    19441 cagaagctcg ttgcctgaca ctttgctaag cgccacggta tcggaaacaa acaccaccgt
    19501 cgtcgcccga tacccaccaa ccatcgcctc gacgaggtac agtcccggct cttgttggcc
    19561 gagaggaata taaatgttac ccggctgcgg cgagatgaaa ttgctggatg cgccttccag
    19621 ttttacccct tgctgcggct cgaccggttt agcctgccat agtggataac ggaactgttc
    19681 caccaaggga tattttttca gcggggaaaa ctggttgttc tgtacataac gggaaggttt
    19741 aataatggca ttgccgagat gtaattcggg taatgcctga gtcacattct gccgtgactg
    19801 agaagagaaa gtacgctgca ttacgcggcg agatttgccg taccagttat cccacagcca
    19861 ggtcagcgta ttgttcagcc cgtcgcccag atattgcggt tgcaccacaa tgcgatgcag
    19921 gtttttctgc tggcgcaaaa atgccatcgg gtcaggaata cgatacaggc gaacgtccac
    19981 gccgccgtac tcttccatct gatagcgccg ataatcacgc cccggcgctt ccagtcgcac
    20041 tttcgcctct tcactgctgc taaaactgct gtcagcaagc aaaaagaatg ttcccccggc
    20101 gggcggcgca tagttactgg aaggaagcga atcatcagca ttagcaagcc ctgttcccac
    20161 aagagacagg gacaagcacg ctgctatcgc gccagaaaat ttaaacgata aatgccaatg
    20221 aaattgggat tggaatcgtt gggtatccat cgggtgtcct tccatgtcat aagttgttgc
    20281 agactgactg cgcgcattcc gttgtcagtt ttcgtggcgc ttccggtgtg gtagatgacg
    20341 taacgcccca tccagaccat taagtgctgg gcatcgccct gatcgaaaaa aatcatatcg
    20401 ccaggcagcg cctggtttat gtcctggcca ataaactggc tgttgtactg aatcaaatta
    20461 atcgcggtca cgtaggggcc ggttttcccg ttcccctgat tccagttttg cgccagttga
    20521 cgctgtccag gtgttagcgt catctctggc ggcaaatact ggctagagaa accgttactt
    20581 tttagccatt tactatcgtg aactttcagc gtctcgttcg ccgcaaatcg caccaggccc
    20641 gcacaatcct gctgatacca gcgtggactt ggcccctggc ggagctgttc ttgtgcaatg
    20701 cgtacaaacc aggcgcgaaa cagcccggat tgttcgacat tcagcatttc gctatgggca
    20761 acaacacaac acagccagca aatcagcgcc agcagcccgt gcctcatagc ggctgccagg
    20821 ttatgggtag ccactgccag gcggcaccgg gttccatctg ggccagcttc atgacataac
    20881 gcggttgttg agataaagcg tccagcttcg gcattaataa agtttgtgcg gcgttataaa
    20941 aaaccggttc gagattcttc ggcagactgg tcaacgtttc gttacgcagc agtttcgcca
    21001 cgccttgtgg attgatatag agcggaacga tgccatcagt gggtattaca tccaccattg
    21061 ccgggcggtt tttattcagt gtttgcaggg cgttattaac cagcgtgtca tcgagggaga
    21121 aaagcagcgt tttgttttgc atcgccagcg acacgcggaa aaaataatcc gacattaatt
    21181 gatcgggttg cgccgcctgc gctttcggat actgaccgta tcgggaactc acttcgcgac
    21241 gccagatttg cgcttcgccc tgctgagtct ggcttatcgg caaaacacct tctggcgctt
    21301 tgctttcgtg cgcaccaata ttttgcgtaa acagtttccc tggcaattgc gcctgttcgg
    21361 cagtgccatc aaactgaccg acaaacagcg gggtttgcaa ttttgagtct gcataccagc
    21421 acagccccgc ggcaccgtct aacgccccat tcaacttgtc gttttcctga ctgatgtgcg
    21481 aaagcatctc ttcggcaata ccgtgtgaat acggcaccac cacacagaag ctggccccgg
    21541 caggcatact gttccagacc ggcgtaaaat cgaaactggt atctacgctg gcggattcat
    21601 catttaacgc cacaaaactg tgccagccgt cgttacccat ttcgaagcgt acgccagcaa
    21661 aagaaggcat taaccgctgg tagccaaaac ccagcaacct ggcgctgacc acgatgcgct
    21721 ggcgtactgg cgttttttca gcagcacgct cttccaggcc aaagcttgct tgccagcgtt
    21781 ttttgccgct caacaaatca cctgcgatcg ccgtggcttc ggtatcctgc tgatcatctt
    21841 taaacaacat atccgtgctg gaaaacacca gcatcttgtc ctgataagtc gcgaacatca
    21901 gggcgttatt gccgttatag cgcaactgat aaacaggaac agtttcacta tttatcttga
    21961 tgctactgat ttccgttttg cttaactggc tgtcgctggt agcggcaaac aacaatggct
    22021 ccagcagttt gcttaaacca ctgcgctgga tcagcaccat ataatgtgaa agatggcctt
    22081 gtttatcgtg ccacagcgct gcctgcgcgg gctgatctaa gagtgacgaa aagagcttat
    22141 ctttcagcgt gagatcgtgt tcatagacaa tacgacgaat gctgccttca atgcccagac
    22201 gatcggcatg attctgataa tagaaaacga aatcttcgct cagaacatcg tggagaaacg
    22261 gaatggtgag gagatctttg ggaagctggc tcagagagtc gctgtcgaga aagaggtccg
    22321 gctcattgag atcgatttgc agattgttgt gcaccaccag cggcgacaac gttttttctg
    22381 gcccactgcc agcatattgc aacgcccaga cgccagcgga aagcagtgct attgcgccaa
    22441 aacctacaag accatagaac cgccagcctt tcgccttttt ttcaccactc attgccacat
    22501 tccttgtgta tagccagcca ttttttacgg gcacagccaa actttaccgt gccctaatac
    22561 gacaaaagcc cagactttgc agcctggact tttcaattca aacaagggag atagctccct
    22621 tttggcatga agaagtaaaa ttattcttct tctggctcgt cgtcaacgtc cacttccgga
    22681 gcgatttcat cgtccccttc cgcggcactg ccatcgatgg tatccagatc ttcctcgtca
    22741 accggttcag caacacgttg cagacccact acgttttcat cttccgcagt acggatgagg
    22801 atcacgccct gggtgttacg gcctacgatg ctgatttccg aaacgcgagt acgtaccagc
    22861 gtaccggcat cggtgatcat catgatctgg tcgcagtcat ctacctgtac cgcgccaacc
    22921 actaaaccat tacgttcagt aaccttaatg gagataaccc ctttcgtcgc acgcgacttg
    22981 gttgggtatt ccgccactgc ggtacgttta ccgtaaccgt tttgcgttgc ggtgaggatt
    23041 gcgccatcgc cacgaggcac gatcagagag acgactttat cgccttcacc taagcgaata
    23101 ccgcgaacac cggtggtgtt gcagcccatc gcacggacag aagactcttt aaagcgcacc
    23161 actttacctt cagcggagaa cagcattact tcgtcttcgc cgctggtcag gtcaacgccg
    23221 atcagctcat cgccgtcaac cagtttgatc gccactttac cggcggtacg cagacggttg
    23281 aactcggtga ggacagtttt cttcacggta ccgttagcgg tcgccatgaa gactttcacg
    23341 ccttcttcaa actcggtcac tggcaggatc gcagtgatac gttcgtcctg ctccagcggc
    23401 agcaggttga cgatcggacg accgcgcgcg ccacgagtgg cttccggcaa ctgataaact
    23461 ttcatcgaat agacgcgacc acggctggag aagcacagaa tatggtcgtg agtgttcgcc
    23521 accagcagtc ggtcgataaa gtcttcttct ttaatacgtg cggcagattt acctttcccg
    23581 ccacgacgct gcgcttcgta ttcagaaagc ggctgatact taacgtagcc ctggtgagag
    23641 agcgtcacta ccacatcttc ctgagtgatc agatcttcca ggttgatgtc tgcgctgttg
    23701 gcggtgattt cagtacgacg tttgtcaccg aactgttcac gaaccagctc cagctcttca
    23761 cggatcactt ccatcaaacg atcggcgcta ccaagaatac gcaacagttc cgcgatctga
    23821 tccagcagct ctttgtattc gtcgagcagt ttttcgtgct caaggccggt cagtttctgc
    23881 aaacgcagat ccagaatcgc ctgagcttgc tgttcggtca ggtagtacag accatcacgc
    23941 acgccgaact ctggctccag ccattccgga cgcgcagcat cgtcgccagc acgttcgagc
    24001 atcgcggcaa cgttgcccag ctgccacgga ttagcaacca gcgcagtttt cgcttctgca
    24061 ggcgtcggcg catgacggat cagttcgatg atcgggtcga tgttcgccag cgccacggct
    24121 aatgcttcaa ggatatgagc acgatcgcga gctttacgca gttcgaaaat agtacgacgg
    24181 gtcaccactt cacggcggtg acgaacaaac gccgcgatga tgtctttcag gttcatgatc
    24241 ttcggctgac catggtgcaa tgccaccatg ttgataccga aagaaacctg caactgggtc
    24301 tgggagtaga ggttgttgag cacaacttca ccgaccgcat cgcgtttcac ttcaatcacg
    24361 atgcgcatac cgtctttgtc agactcgtca cgcagcgcgc tgatgccttc cacgcgtttt
    24421 tcttttacca gttccgcaat cttctcgatc aggcgcgctt tgtttacctg atacggaatt
    24481 tcgtggacga taatggtttc acgaccggtt ttggcgtcaa cttccacttc tgcgcgagcg
    24541 cggatataca ccttgccgcg accggtacgg taagcttctt caataccgcg acgaccgtta
    24601 atgattgccg ccgtcgggaa gtccggcccc gggatgtgtt ccatcagccc ttcaatgctg
    24661 atgtcttcat catcgatata cgccagacaa ccgttgatga cttccgtcag gttgtgcggc
    24721 gggatgttgg ttgccatacc tacggcgata ccggaagaac cgttcaccag caggttagga
    24781 attttggttg gcatgacgtc gggaattttt tccgtgccgt catagttatc aacgaaatcg
    24841 accgtctctt tttcgagatc ggccatcagt tcatgggcaa ttttcgccag acggatttcc
    24901 gtataacgca ttgccgccgc agagtcgccg tcgatggaac cgaagttacc ctgaccgtct
    24961 accagcatgt aacgcagcga gaatggctgc gccatacgga cgatcgtgtc ataaaccgcc
    25021 gagtcaccat ggggatggta tttaccgatt acgtcaccaa cgacacgggc agatttttta
    25081 taggctttgt tccagtcatt gcctagtacg ttcatggcgt aaagtacgcg acggtgtacc
    25141 ggcttcaggc catctcggac atctggcagc gcacggccaa caatgaccga catcgcataa
    25201 tccagatagg agctcttcag ctcttcctca atgttgaccg gtgtaatttc tctcgcaagg
    25261 tcgctcatct aaccgctatc cctctactgt atcccggatt caaaggtcgc aaattataac
    25321 acagccgcgc agtttgaggt aaacctatac gctttattca catccaatgc ctgatatact
    25381 cgtttgtctt gccaattacg gagtagaagt gccaatgaat gccgaaaaat cgccggtaaa
    25441 ccataacgta gaccacgaag agatcgctaa atttgaagcc gtcgcctccc gctggtggga
    25501 tctggaaggt gagttcaaac cgctgcaccg cattaacccg ctgcgtctgg gctatattgc
    25561 cgagcgtgct ggcggtttat ttggcaaaaa ggtgctcgat gtcggttgtg gcggcggcat
    25621 tctggccgag agtatggcgc gcgaaggcgc gacggtgacc ggtctggata tgggctttga
    25681 gccattgcag gtggcaaaac tgcacgcact ggaaagcggc attcaggtgg attacgtgca
    25741 ggaaaccgtg gaagagcacg cggcaaaaca tgccgggcag tatgatgtgg tgacctgcat
    25801 ggagatgctg gagcacgtcc ccgatccgca gtcagtggtc agagcctgtg cgcaactggt
    25861 gaaaccgggc ggcgatgtct ttttctcgac acttaaccgc aacggcaagt catggctgat
    25921 ggcggtggtt ggtgcggaat atattttgcg catggtgccc aaaggcacgc atgatgtgaa
    25981 gaagtttatt aaaccggcag aattgctggg ctgggtagat cagacaagcc ttaaagagcg
    26041 acatataacc gggctacatt acaacccgat cactaatact tttaaactcg gccccggcgt
    26101 ggatgtgaac tatatgctgc acacgcagaa taagtgaggt tgttgtttgg ccgagccaat
    26161 gcctgatgcg acgcttgccg cgtcttatca ggcctacaaa tgctccccgt aggccggata
    26221 aggcgtttac gccgcatccg gcaaccgtgc cgactagaca gtgattacca tttcaccgtc
    26281 atcgacaaaa accctgccgt ctgggcaaaa tcatcactcc ctttctgcca cgccacgctg
    26341 ccgcgcaggg acactcgctg actgatattg cccgtgactc ccacttttat ttcaccccgt
    26401 cgcttcaccg catcgtcact gatagtgctg ccatcttctt caatttccgt cgcatgggga
    26461 tcgtgataat aattcagatc taatgttggt gtgacaccaa ccgcggtacg ccattcgctg
    26521 tgtaaaccca gccgcgtctg aatatcatcc ccctgcgatt gtgacacgcg cgcatggtta
    26581 gcggcggtaa aatcatcctg ctgcacgccc tgataaatca cctgcgcctg cggttcaatc
    26641 accacaccac gccccggtaa ccactgatac ccggcttcca gcgaggcgat aatccccgac
    26701 gagtggtaat gatctgtgcc atcgtcttgt tcggaaacat cattgttaaa ccacgcgtat
    26761 tgcagccagc tatccagcca ggccccctgc ttctgattac cgtgctgata ccagcttgag
    26821 gtcagcccaa cggcataacc gtggttctgg ttatcggcgc gagttccggt catattcgag
    26881 cggctctcgc cctgattatc actgtagcca ccaaccgccc caagcatcca ctcgccatca
    26941 tcgccccagc gcccgctaaa caggtcaccg ctaagctgca ccgtagaagt gtcttcatgc
    27001 tgagccagtt gccctgccgc cgtgtaatga taacgtccgc cgataacacg taaattcagc
    27061 gtctgaccat tgccacctgc atgatcgcgt cgctccatca taaacgcctg atttgccgcc
    27121 cgcagattat tgagataacc gccaactttg gcattcagca ccggctggta agcggggatt
    27181 ggatcgggtg ttgggtcagg atcgggtgtt gggtccgggt ctggatcagg tggcgatggc
    27241 ggcgttactt cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca
    27301 tacgctccca tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac
    27361 tgcgtgggat cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc
    27421 cctgtaatgg agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat
    27481 tgatcgctta ccgagtcatc gccgtttaat tcgctgtcga acagtagcgt accgccgccg
    27541 gtataatcac cgtttaccgt cagcgtatcg ccagcgacgc catttttcag gctaaccgca
    27601 ccggacgtat tggtcagatc accattaacc gtcacgtcgg caagataggt caggctgtca
    27661 gtttcaaggt tggtattaag ctcacctgtc aacgtcacgc catcggtagc attcaccagc
    27721 gtcccatcgc cgttaaacaa tgaggcatta agggcgacgg aagtatcctg cccggtcaga
    27781 cgcaaaaccg taccatcgct gatatcgata gtgccgctgg aagtagcatc gatagactga
    27841 atatcctgat ccgcgccagt aacgaacgtc gccccatcac cgacccacag tgacgaagca
    27901 taaggaaaga tatccgcaac atcgcctttc agggtgcctt cttcgacacg tactgccgac
    27961 tgcgttgtac cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta
    28021 ccctcatcct gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc
    28081 cccgcatcaa ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc
    28141 gtcgtagagc gagtatcgac attggcagta atctccatca cgctttcttg ttgcttatcg
    28201 gtaaagataa cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc
    28261 agcgtaccgg aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc
    28321 tgcccgtcgc tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc
    28381 aaattgtcgc cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg
    28441 ccactgactt caaggccatc attgtatgat gtgccgctct gccaggtgga gaaatcagag
    28501 agatcgagca caccgccgtt taacacgatc gactgcggat cgtcctggag agcggcgaga
    28561 tctgccgcgt cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt
    28621 agcgccatcg actgcgcccc cgacagcacg tagctgccgt tttgcgcaat ggtgagctga
    28681 ccagcacctt cgatggtgcc agcaaaactg ccttgattaa cagtaacatt gccaccagca
    28741 tcgatattta aagtgccatt ctgaaagccc gtcaatgagt gcacaaaggt ttgttgggtg
    28801 gagccaacgt ttagctctgc ctgattctgg tacttatcaa tactccctat cgtcagaccg
    28861 tagcagtctt gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg
    28921 cggcccaggg taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct
    28981 gcattaagta ccagatcgcc ggaacctgtt ttggtgatca acccggtacc agcaatagag
    29041 tcaacagctc cgtcattctc tgtattgcca ataaccagcg tttttccgtc ggcaatatca
    29101 aaggtaactt cacttaagcc gagatacata aagccacccg ccgcagtgga aggaccatct
    29161 ccatagcctg ctgcgctgtt gttctcatcc actaacacgc cgccgttctg gctgtagctg
    29221 tcatcaacag aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg
    29281 ccataacctt cggcagtgtt atttgtaaag gcagtgttat ttattatcgt ataacctgaa
    29341 ggatgcttgc tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg
    29401 taacttgttg atgtatatgc ctggttgtta tcaaaaataa catcacttaa atagacgtca
    29461 ttattaatgg tataaattgc gccacctttg ccatcattag cgatattatt gcgaaacatg
    29521 gcgttagtga cacgtaaatc gacggcacca gtatcattag taccagaaga atagattgcg
    29581 ccaccatcgc cgcctgcgac gttaccagaa aaaataacat cagtaagatt cagcgttgag
    29641 ttttctttag caaatatggc cccgccgtta ttatattctc ctgtaacggt gttattggca
    29701 aacagagtca ttccagtttc attttctggt aatagtgaaa attccgctcc ttgttgcaaa
    29761 aatacggctc ctccgctggc gttattggtc atatccgaaa aaaccagcca ttgcccatcg
    29821 gcgacactcc agtcctgcgt aatgcctgaa aggctttgcc tgctggcgtg acaactcgct
    29881 ttgatatcat atccctggca taaatcaatg gccgccgcga aaccgttagc agagaaaaga
    29941 gatgcaatca ttgacgggag tagagataaa tactccttgc gtagaaagat aatccgcata
    30001 ttcatctaac catcatttta tataagacgg catatttaaa cgcattatac aatatgggtt
    30061 atctttcttt tgtaaattgt ccaacccgat gcaatggcta ttttgttatt atttgatgaa
    30121 tagtatcagt gcgtcataat tcaagttaat aaccttaagg gataccagtt atatttaaac
    30181 taaattaaag taatgaataa ttttcttata atataaggta aattaacaaa atggcttcgc
    30241 atttaacaat aaccgaatag aaaacaacca tttcgacatc aacaatcgct tacattcgtt
    30301 tatatattga ccacaactga tacatcagat tatgtgatga ctcgtgctta gatcaatttt
    30361 tgcaatcatt agcaaaaaga ttaataaacc atctatatca atttatccaa cctattctgc
    30421 cgttcaagaa atcgccgaac agttattttt aacaaatttt tctcttccca ttgactttcc
    30481 cggacacctt gtctgaccta aggtgcgcga aagccacttt ttccttcctg agttatccac
    30541 aaagttatgc acttgcaaga gggccatttt cacactatct tgcagtgaat cccaaacata
    30601 ccccctatat atagtgttct aagcagcttc ccttactaca ggtagtctgc atgaaactat
    30661 tgcggaaaga attccaaaaa caggtacgac atacatgaat cagaatctgc tggtgacaaa
    30721 gcgcgacggt agcacagagc gcatcaatct cgacaaaatc catcgcgttc tggattgggc
    30781 ggcagaagga ctgcataacg tttcgatttc ccaggtcgag ctgcgctccc acattcagtt
    30841 ttatgacggt atcaagacct ctgacatcca cgaaaccatt atcaaggcgg ccgcagacct
    30901 gatctcccgt gatgcgccgg attatcagta tctcgccgcg cgcctggcga tcttccacct
    30961 gcgtaaaaaa gcctacggcc agtttgagcc gcctgcgctg tacgaccacg tggtgaaaat
    31021 ggtcgagatg ggcaaatacg ataatcatct gctggaagac tacacggaag aagagttcaa
    31081 gcagatggac acgtttatcg atcacgaccg tgatatgacc ttctcttatg ctgccgttaa
    31141 gcagctggaa ggcaaatatc tggtacagaa ccgcgtgacc ggcgaaatct acgaaagcgc
    31201 ccagttcctt tatattctgg ttgccgcgtg cctgttctcg aactacccgc gtgaaacgcg
    31261 cctgcaatat gtgaagcgtt tttacgacgc ggtttccaca tttaaaattt cgctgccgac
    31321 gccaatcatg tccggcgtgc gtaccccgac tcgtcagttc agctcctgcg tactgatcga
    31381 gtgcggtgac agtctggatt ccatcaacgc tacctccagc gcgattgtga aatacgtttc
    31441 ccagcgtgcc gggatcggca tcaacgccgg gcgtattcgt gcgctgggta gcccgattcg
    31501 cggtggtgaa gcgttccata ccggctgtat tccgttctac aaacatttcc agacagcggt
    31561 gaaatcctgc tctcagggcg gcgtgcgcgg cggtgcggca acgctgttct acccgatgtg
    31621 gcatctggaa gtggaaagcc tgctggtgtt gaaaaacaac cgtggtgtgg aaggcaaccg
    31681 cgtgcgccat atggactacg gggtacagat caacaaactg atgtataccc gcctgctgaa
    31741 aggtgaagat atcaccctgt tcagcccgtc cgacgtaccg gggctgtacg acgcattctt
    31801 cgccgatcag gaagagtttg aacgcctgta taccaaatat gagaaagacg acagcatccg
    31861 caagcagcgt gtgaaagccg ttgagctgtt ctcgctgatg atgcaggaac gtgcgtctac
    31921 cggtcgtatc tatattcaga acgttgacca ctgcaatacc catagcccgt ttgatccggc
    31981 catcgcgcca gtgcgtcagt ctaacctgtg cctggagata gccctgccga ccaaaccgct
    32041 gaacgacgtc aacgacgaga acggtgaaat cgcgctgtgt acgctgtctg ctttcaacct
    32101 gggcgcaatt aatagcctgg atgaactgga agagctggca attctggcgg ttcgtgcact
    32161 tgacgcgctg ctggattatc aggattaccc gatcccggcc gccaaacgtg gagcgatggg
    32221 tcgtcgtacg ctgggtattg gtgtgatcaa cttcgcttac tacctggcga agcacggtgt
    32281 gcgttactcc gacggcagcg ccaacaacct gacgcataaa accttcgaag cgattcagta
    32341 ttacctgctg aaagcgtcta acgagctggc aaaagagcaa ggcgcgtgcc cgtggtttaa
    32401 cgaaaccact tacgcgaaag ggatcctgcc gatcgatacc tataagaaag atctggatac
    32461 catcgctaat gagccgctgc attacgactg ggaagcattg cgtgaatcaa tcaaaacgca
    32521 cggcctgcgt aactccacgc tttctgccct gatgccgtca gagacttctt cacagatctc
    32581 taacgccact aacggtattg aaccgccgcg cggttacgtc agcatcaaag cgtcgaaaga
    32641 cggtattttg cgccaggtgg tgccggacta cgagcacctg cacgacgcct atgagctgct
    32701 gtgggaaatg ccgggtaacg atggctacct gcaactggta ggtatcatgc agaaatttat
    32761 cgatcagtcg atctctgcta acaccaacta cgatccgtca cgtttcccgt caggaaaagt
    32821 gccgatgcag cagttgctga aagacctgct caccgcctac aaattcgggg tcaaaacgct
    32881 gtattatcag aacacccgtg acggcgctga agacgcgcag gacgatctgg taccgtcaat
    32941 ccaggacgat ggctgcgaaa gcggcgcatg taagatctga tattgagatg ccggatgcag
    33001 cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc ctgataagac gcgtcagcgt
    33061 cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg cattaaactc ccaacaggac
    33121 acactcatgg catataccac cttttcacag acgaaaaatg atcagctcaa agaaccgatg
    33181 ttctttggtc agccggtcaa cgtggctcgc tacgatcagc aaaaatatga catcttcgaa
    33241 aagctgatcg aaaagcagct ctctttcttc tggcgtccgg aagaagttga cgtttctcgc
    33301 gaccgtatcg attaccaggc actgcctgaa cacgaaaaac acatctttat cagcaacctg
    33361 aaatatcaga cgctgctgga ttccattcag ggccgtagcc cgaacgtggc actgctgccg
    33421 ctgatctcga tcccggaact ggagacctgg gtcgaaacct gggcgttctc agaaacgatt
    33481 cattcccgtt cctatactca tatcattcgt aatatcgtta acgatccgtc tgttgtgttt
    33541 gacgatatcg tcaccaacga gcagatccag aaacgtgcgg aagggatctc cagctattac
    33601 gatgagctga tcgaaatgac cagctactgg catctgctgg gcgaaggtac ccacaccgtt
    33661 aacggtaaaa ctgtgaccgt tagcctgcgc gagctgaaga aaaaactgta tctctgcctg
    33721 atgagcgtta acgcgctgga agcgattcgt ttctacgtca gctttgcttg ttccttcgca
    33781 tttgcagaac gcgaattgat ggaaggcaac gccaaaatta ttcgcctgat tgcccgcgac
    33841 gaagccctgc acctgaccgg cacccagcat atgctgaatc tgctgcgcag cggcgcggac
    33901 gatcctgaga tggcggaaat tgccgaagag tgtaagcagg agtgctatga cctgtttgtt
    33961 caggcagctc aacaggagaa agactgggcg gattatctgt tccgcgacgg ttcgatgatt
    34021 ggtctgaata aagacattct ctgccagtac gttgaataca tcaccaatat ccgtatgcag
    34081 gcagtcggtt tggatctgcc gttccagacg cgctccaacc cgatcccatg gatcaacacc
    34141 tggctggtgt ctgataacgt gcaggttgct ccgcaggaag tggaagtcag ttcttatctg
    34201 gtcgggcaga ttgacgcgga agtggacacc gacgatttga gtaacttcca actctgatgg
    34261 cccgcgttac cctgcgcatc actggcacac aactgctgtg ccaggatgaa cacccttccc
    34321 ttctggcggc gctggaatcc cacaatgtgg cggttgagta tcagtgtcgc gaaggttact
    34381 gcggctcctg tcgcacccgt ctggttgcag gtcaggttga ctggattgcc gaaccgttag
    34441 cctttattca gccgggggaa attttgccct gttgttgccg ggcaaaaggc gatattgaaa
    34501 tcgagatgtg aattggttgt agtgccagat acaacgctta tgcgtcttat ctggcctaca
    34561 acgattacat ggcgtagtaa tacgctttca cctgctccca gtccgctttg gggattggct
    34621 ccagatattt ttccagctgg cggaagtcat gattaatcgc tttatcgcga cgcaagcggc
    34681 gacgactttt ctccaggtca agaaaaccag cttctgcatt accttctgtt ttcacataga
    34741 tatggcgaac ataacaacag ccatgctgac gattaatgct atgcattttc ttaaacgcca
    34801 gcgccactgc tttcaacatg gcttgccgta cttcgtcaga ataaggcgat actgcatgct
    34861 gggcatacca gtcagcaatg ctgatgaacc ccgccatatc ttcagtcacc agcaacgctc
    34921 gccattcacc ctcaattttc accgcttcac caaaaacgat cttcggcacg atgacaccgg
    34981 cccgttccag ttctttaatt accgcaacct cacggacaat cgttggtcgg ccgaacggat
    35041 aacgtacgga atgaaacaga tgatgcgtca tgcgctttac atacagcttt ttgccgttgc
    35101 gctcgacgca ttgcaccccg ctcataccat tacggcgata gttaggctct tcaacccagt
    35161 cgccctctgt tgcccaccag tgattaaatt cgtcgtactt tgctgaaact gccataccct
    35221 atcgcctgtc atttttatta acgacaatga ctataggtgg ttacctgagg aaaatcttaa
    35281 tgaaacgtgt cgtattaatg aatttctttt gccactacta gcgtgacacc gcttttaccc
    35341 ttcatttgca gcgcatagat tccaccagcc actaccacta atagtgcgcc cagtagataa
    35401 aatgtgaatt taatcagtcg gatacagcga tgaagtttat tcgacaggga ttaggcatcg
    35461 ccttacaacc agagttaacg ctgaaaagca ttgcaggtga attgtgttcc gttcctcacg
    35521 aaccaacctt ctatcgacag atttcgttgt tggctaaaga aaagccggta gaaggcagtc
    35581 cactgttttt actacaaacg tgtacggaac aactcgtggt gaatggaaaa atttgatgtg
    35641 agacaggatg gcgcatgaac gccatcctgc agtagcatta ctctttatta aggaatttta
    35701 ctgccttatc agggaaatca gtaaacagcc catttacacc cgctttgtta tacagagcat
    35761 catataactg attcacatca gtagtgtatt caggcagttt atctgaccgc acggtataag
    35821 gatgcactac cagtttattc tgctgagcat cttgcaccat gccagtgagt ttgatattac
    35881 ccggctgcga tgtttcctca atcaacatat ggtaatccgg cccgataccg tcggcatatt
    35941 ccgccacctg tttcatagca cccggcttaa acatccagtc gtagttgtaa ttaacccagc
    36001 ttccatccgg ctgtttctgc tgcgtttcat tccagtcggt ataggcaatc agctgtacca
    36061 gattgagatc catgcccatt ttgggttcca gctcattctt aatacgcttc agctcatcag
    36121 catcaaaaca ttgcaaataa actttgtcgt ccttaccggt gtaaccatat ttcttcagca
    36181 cttccagcgt ttttgccgca atatccttcc cttcctgatg atggaaccac ggcgctttaa
    36241 tttctggata gataccgata tttttcccgg tagagtgatt taacccctga acaaattcaa
    36301 tctcttcttc aaaggtgtgc acccggaagt cggacttacc cattgggaaa cgccccggat
    36361 aagtctgtac ttttttaccg ttttcaatat cgaaaccttc ggtaaatttc agcgacttaa
    36421 tttcatccag cgtgaaatct atcgcgtagt aacgaccgtc tttgcgcgcc cgatccggga
    36481 aacgatcggc aacatcagta acacgatcga gataatggtc atgcagaaca accagatggt
    36541 cgtctttggt catcaccaaa tcctgttcca gataatccgc tccctgcgca tacgccatcg
    36601 cttttgctgg cagcgtatgc tccggcaaat atccactggc accgcgatgg gcgatgacta
    36661 ttttttcgtt gctgtccgcc gccattgcac tgcttcccat gactatagtg ctcatcatga
    36721 tcgccatgct aaggtttttc agcttcaatt tcatgccatt agcctccgtt gcgtttttgc
    36781 agtaattgtt catggcgacg tttttcgcca atcatcacaa caatcaacaa gataaccgcc
    36841 agaatgctgc cgccaatcat taccataaag ccgccatccc agccgaagaa gtccacggtg
    36901 tagccaacaa tcgcgctcgc cgccaccgaa ccgcccaggt aaccaaacag cccggtaaag
    36961 cccgctgccg tacctgccgc ttttttcggt gccagttcca gcgcatgcag accgatcagc
    37021 atcacaggac cgtagatcag gaagccgata acaatcatac aaatcatatc gacggttggg
    37081 ttacctgccg ggttcatcca gtaaacgata gtcgcgatgg tcaccagtgt cataaagaaa
    37141 acgccggttg ccccacggtt gccacggaag actttatccg acatccagcc gcacagcagg
    37201 gtgcccggaa tacccgcata ctcatagaag aagtaggccc aggaagattt atccagcgcg
    37261 aaatgtttaa cctctttcag gtaagtcggt gaccagtcga ggatgccgta acgcagcaga
    37321 taaacgaaca cgttggcgat ggcgatatac cacagcaatt tgttcggcaa cacgtactgc
    37381 atgaagatct gcttcgccgt cagttcctgt tccgcttttt cgttatagtc gtccgggtaa
    37441 tcatttttgt actcttcgat cggcggcaaa ccacaagatt gcggggtatc gcgcatcatc
    37501 gcaaaggcga ataatgccac cagaatggcg cagaaagcgg gcatatagag cgccgcatgc
    37561 cagtcattga accaggccat ccccagcagg aacagcagcg gcggaatacc accaccgacg
    37621 ttgtgcgcac agttccacac tgacacaatg ccgccacgtt ctttctgcga ccaccagtgc
    37681 accatagtac gaccacacgg cggccacccc atcccctgga accaaccgca gaggaacaac
    37741 agtacaaaca tcaccgcaat gctcgacgtc gcccatggca caaagcccat aaacaacatc
    37801 actgccgccg ccagaatcaa acctgcgggc aggaaaacgc gcggattcga gcgatccgat
    37861 accgaaccca tgatgaattt cgaaaatcca taagcaatcg agatccccga aagggcaaaa
    37921 cctaaatcac cgcgtgagaa tccctgctca accagataag gcatagcaag cgcaaagttc
    37981 ttacgaacca aatagtaagc cgcatagcca aagaatatcc ccaggaaaat ttgccagcgc
    38041 aatcgacgat aagtcggatc gatctccgcg gcaggtaagc gcgctttgtg tggcgctggt
    38101 ttaaaaatac tcaacattga tagcctccgt ggcccgtggt cttatttatg attaacagcc
    38161 tgattcagtg agagaacctg ccgtttcttg agttgccgcg atgttaagaa aacattcata
    38221 aattaaatgt gaattgccgc acacattatt aaataagatt tacaaaatgt tcaaaatgac
    38281 gcatgaaatc acgtttcact ttcgaattat gagcgaatat gcgcgaaatc aaacaattca
    38341 tgtttttact atggctaaat ggtaaaaaac gaacttcaga gggataacaa tgaaaactcg
    38401 cgactcgcaa tcaagtgacg tgattatcat tggcggcggc gcaacgggag ccgggattgc
    38461 ccgcgactgt gccctgcgcg ggctgcgcgt gattttggtt gagcgccacg acatcgcaac
    38521 cggtgccacc gggcgtaacc acggcctgct gcacagcggt gcgcgctatg cggtaaccga
    38581 tgcggaatcg gcccgcgaat gcattagtga aaaccagatc ctgaaacgca ttgcacgtca
    38641 ctgcgttgaa ccaaccaacg gcctgtttat caccctgccg gaagatgacc tctcattcca
    38701 ggccactttt attcgcgcct gcgaagaagc agggatcagc gcagaagcta tagacccgca
    38761 gcaagcgcgc attatcgaac ctgccgttaa cccggcactg attggcgcgg tgaaagttcc
    38821 ggatggcacc gttgatccat ttcgtctgac cgcagcaaac atgctggatg ccaaagaaca
    38881 cggtgccgtt atccttaccg ctcatgaagt cacggggctg attcgtgaag gcgcgacggt
    38941 gtgcggtgtt cgtgtacgta accatctcac cggcgaaact caggcccttc atgcacctgt
    39001 cgtggttaat gccgctggga tctgggggca acacattgcc gaatatgccg atctgcgcat
    39061 tcgcatgttc ccggcgaaag gatcgctgct gatcatggat caccgcatta accagcatgt
    39121 gatcaaccgc tgccgtaaac cttccgacgc cgatattctg gtgcctggcg ataccatttc
    39181 gctgattggc accacctctt tacgtattga ttacaacgag attgacgata atcgagtggc
    39241 ggcagaagag gttgatattc tgctgcgtga aggggaaaaa ctggcccccg tgatggcgaa
    39301 aacgcgcatt ttgcgggcct attctggcgt gcgcccgctg gttgccagcg atgacgaccc
    39361 gagcggacgt aacgtcagcc gtggcatcgt gctgctcgac catgctgaac gcgatggtct
    39421 ggacggattt atcaccatca ccggtggcaa actgatgacc tatcggctga tggctgaatg
    39481 ggctaccgac gcggtatgcc gcaaactggg caacacgcgc ccctgtacga ctgccgatct
    39541 ggcactgcct ggttcacaag aacccgctga agttaccttg cgtaaagtca tctccctgcc
    39601 tgccccgctg cgcggttctg cggtttatcg tcatggcgat cgcacgcctg cctggctgag
    39661 cgaaggccgt ctgcatcgta gcctggtatg tgagtgcgaa gcggtaactg cgggtgaagt
    39721 tcagtacgcg gtagaaaatt taaacgttaa tagcctgctg gatttacgcc gtcgtacccg
    39781 tgtggggatg ggtacctgcc agggcgaact ctgcgcctgc cgcgctgccg gactgctgca
    39841 acgttttaac gtcacgacgt ccgcgcaatc tatcgagcaa ctttccacct tccttaacga
    39901 acgctggaaa ggcgtgcaac ccatcgcctg gggagacgca ctgcgcgaaa gcgaatttac
    39961 ccgctgggtt tatcagggac tgtgtggtct ggagaaggag cagaaagatg cgctttgata
    40021 ctgtcattat gggcggcggc ctcgccggat tactctgtgg cctgcaactg caaaaacacg
    40081 gcctgcgctg tgccattgtc acgcgtggtc aaagcgcgct gcatttctca tccggttcgc
    40141 tggatttgct gagccatctg cctgatggtc aaccggtgac agacattcac agtggactgg
    40201 aatctttgcg tcagcaggca ccagcccatc cttacagtct tctcgaacca caacgcgtgc
    40261 tcgatctcgc ttgccaggcg caggcattga tcgctgaaag cggtgcgcaa ttgcagggca
    40321 gcgtggaact ggcccatcag cgcgttaccc cgctcggcac tttgcgtgca acctggttaa
    40381 gttcgccaga agttcccgtc tggccgctac ccgcgaagaa gatatgtgta gtggggataa
    40441 gcgggctgat ggatttccag gcgcatcttg cggcggcttc gttgcgcgaa ctggatcttt
    40501 cggttgaaac cgcagaaata gagctgccgg aactggatgt cctgcgcaat aacgccaccg
    40561 aatttcgcgc tgtgaatatc gcccgtttcc ttgataatga agaaaactgg ccgctgttac
    40621 ttgatgcgct tattcctgtc gccaatacct gcgaaatgat cctgatgccc gcctgcttcg
    40681 gcctggccga tgacaaactg tggcgttggt tgaatgaaaa actaccttgt tcactgatgc
    40741 ttttgccaac gctgccgcct tccgtgctgg gcattcgtct gcaaaaccag ttacagcgcc
    40801 agtttgtgcg ccagggtggc gtgtggatgc cgggcgatga agtgaaaaaa gtgacctgta
    40861 aaaatggcgt agtgaatgaa atctggaccc gcaatcacgc cgatattccg ctacgtccac
    40921 gtttcgcggt tctcgccagc ggcagtttct ttagtggcgg actggtagcg gaacgtaacg
    40981 gcattcgcga gccgattctc ggccttgatg tgctacaaac cgccacgcgg ggtgaatggt
    41041 ataagggcga tttttttgcg ccgcaaccgt ggcagcagtt cggtgtaacc actgatgaga
    41101 cgctacgccc gtcgcaggca gggcaaacca ttgagaacct gtttgccatc ggttcggtgc
    41161 tgggcggatt tgatcccatc gcccagggat gcggcggcgg tgtttgtgcc gtcagtgctt
    41221 tacatgccgc tcaacagatt gcccaacgcg caggaggcca acaatgaacg acaccagctt
    41281 cgaaaactgc attaagtgca ccgtctgcac caccgcctgc ccggtgagcc gggtgaatcc
    41341 cggttatcca gggccaaaac aagccgggcc ggatggcgag cgtctgcgtt tgaaagatgg
    41401 cgcactgtat gacgaggcgc tgaaatattg catcaactgc aaacgttgtg aagtcgcctg
    41461 cccgtccgat gtgaagattg gcgatattat ccagcgcgcg cgggcaaaat atgacaccac
    41521 gcgcccgtcg ctgcgtaatt ttgtgttgag tcataccgac ctgatgggta gcgtttccac
    41581 gccgttcgca ccaatcgtca acaccgctac ctcgctgaaa ccggtgcggc agctgcttga
    41641 tgcggcgtta aaaatcgatc atcgccgcac gctaccgaaa tactccttcg gcacgttccg
    41701 tcgctggtat cgcagcatag cggctcagca agcacaatat aaagaccagg tcgctttctt
    41761 tcacggctgc ttcgttaact acaaccatcc gcagttaggt aaagatttaa ttaaagtgct
    41821 caacgcaatg ggtaccggtg tacaactgct cagcaaagaa aaatgctgcg gcgtaccgct
    41881 aatcgccaac ggctttaccg ataaagcacg caaacaggca attacgaatg tagagtcgat
    41941 ccgcgaagct gtgggagtaa aaggaattcc ggtgattgcc acctcctcaa cctgtacatt
    42001 tgccctgcgc gacgaatacc cggaagtgct gaatgtcgac aacaaaggct tgcgcgatca
    42061 tatcgaactg gcaacccgct ggctgtggcg caagctggac gaaggcaaaa cgttaccgct
    42121 gaaaccgctg ccgctgaaag tggtttatca cactccgtgc catatggaaa aaatgggctg
    42181 gacgctctac accctggagc tgttgcgtaa aatcccgggg cttgagttaa cggtgctgga
    42241 ttcccagtgc tgcggtattg cgggtactta cggtttcaaa aaagagaact accccacctc
    42301 acaagccatc ggtgcgccac tgttccgcca gatagaagag agcggcgcgg atctggtcat
    42361 caccgactgc gaaacctgca aatggcagat tgagatgtcc acgagccttc gctgcgaaca
    42421 tccgattacg ctactggccc aggcgctggc ttaaccgacc ttttgatgcc cggtaagcac
    42481 attgcttatc gggcattttt gcgagcgcga ttccacgcac catgtatgcg ttgcaacgct
    42541 ttgaaaattc ctctgaaaat gtctcgcaaa ggctgaaact gacagatgtc aaaggcccgg
    42601 gatagacgta atgtcgcgtc atcataaata tcaggtgacg gacaaccatg accgaatcaa
    42661 caacatcctc cccgcatgat gcggtgttta aaacctttat gttcacaccc gaaaccgcac
    42721 gggattttct cgaaatacat ttaccagaac cactgcgcaa gctttgcaac ctgcaaacct
    42781 tacgcctgga acccactagt tttattgaaa aaagtttacg cgcttactac tcggatgttt
    42841 tgtggtccgt ggaaaccagc gacggtgacg gctatatcta ctgcgtgatt gaacatcaaa
    42901 gctctgcaga aaagaatatg gcttttcggc taatgcgcta tgccactgcc gccatgcagc
    42961 gtcacctgga taaaggctat gacagagttc cgctggtggt gccattgctg ttttatcatg
    43021 gcgaaacatc gccctacccg tactcactta actggctgga tgagtttgac gatccgcaac
    43081 ttgcccggca gttgtacacc gaagcttttc cgttggtgga tatcaccatc gtacctgacg
    43141 atgagatcat gcaacatcgg cgtatagctc tgctggaact gattcaaaag catattcgcg
    43201 accgcgattt aatcggcatg gtcgacagga tcaccacgct tttggttaga ggcttcacta
    43261 atgacagcca gctacaaaca ctgtttaatt atctgctgca atgcggcgat acctcccgtt
    43321 tcacccgttt tattgaggag attgccgaac gttcaccact acaaaaggag agattaatga
    43381 ctattgctga acggctacgg caggaagggc atcaaattgg ctggcaggaa ggtatgcatg
    43441 aacaagccat taaaattgct ttgcgcatgc tggaacaggg cattgatcgt gaccaggtgc
    43501 tcgcggccac ccagctaagc gaagccgatc tggcagcgaa taaccactaa ttaacacagg
    43561 cccacagccg atccccatgg gcctttgata tcaataacta ccttttatgc gtggcccatt
    43621 tttgcctgat ttaaacatcg ccagtcgttg atccagggca tcgctgtaga gcatcgtgtc
    43681 aacgccaaca gcgacaaagt tcgctcccca cgccaggcat tgctgcgcca tatcaggagc
    43741 cacagccaga aaaccagccg ctttacccgc agcacggatc cgccgaatac tggtttcaat
    43801 aattcgctgc acttccgggt gccctgcgtt atccgggtag cccaacgacg cagaaagatc
    43861 cgcaggtcca ataaacacgc catcaatccc ttcgacgtcg aggatttcgt ccaggttatc
    43921 cagtgccgtt ttactttcca cctgcaccaa cagacaaagt gaatcgttaa cttgcgccat
    43981 gtaattctca atgcgtcccc agcgcgcagc ccgtgccaca ctggccccaa caccacgctc
    44041 accgtaggga gggttggaca agttttgccg gagggggaat gtgccggtgc agtaaaccgg
    44101 cacagagtgg cggaatcaga cagataaggt catggactca ataacctcaa tccagccatg
    44161 ctcactggcg atatcctggc cgtttaacca acggcgcagc atattcagtg ccatcattgc
    44221 gcacacttcc tgacggatag ccaggctgta gcgtgtagtg ctgaaacgca cacgcagagc
    44281 gaaagtgccg tctggcgtgg ctagcgcaaa gttgagatgc tcgttctcga aacccgaaac
    44341 agccagtgcc agcccggcaa aatggttggc ccgccgttct gtaatccagt gcgcagtttg
    44401 cgccagggtt tcttcctgtg aaggaaccac ttcacacgcc agcaatggag cacctgcgcg
    44461 agaaagttgc aaagccaata aaccgccggt gaattgctcg ctcaacgtca ggctgaactg
    44521 gcggttttgc aattcgcgac tgatctgcgc gggcagtcct tcggtgcctt cgaaaatcac
    44581 gctctgtccg gcaacacgtt taacgtccag ccacagtttt tccatcgcct gttgctcgct
    44641 tgccggtccg gtgagtttca gttcgataat cggcattgaa gagcgatagc ccattgttac
    44701 gcccggcggc agttgtagag tgtccaggct ttgtgccaga tcgctttccg aacgaccaaa
    44761 ggtagttaaa cgcagacaaa ccggcggctg tggtaaagaa aaacgctcgc gcagacgcgg
    44821 caggatttcg tgctcgacca tcaccttaaa ttctgacggt acgccgggag taaagaacat
    44881 caggcaacga ttaagctgca cggcaaaacc acaggcggtg cctaccgggt tattgataaa
    44941 ttcagcactg gcaggcagct ccgcttgttt acggttgctc ggtgccatta ctcgtccacg
    45001 ttcgtggaaa tagcgttcca tctctttgag ccaggcttca tgcagcacca tgccttcacc
    45061 ttttgctgtc gcagcggcga gtgcgcttaa atcatcgctg gtcggtccca gcccgccgtt
    45121 aacgatcagc acatcggcgt gctgactacg ttcgcgaaga atggtgacta agtcatcaag
    45181 gttatccccc accgtattgc ggcgagataa tggcagcccc tgatgaaaga aaaaatcggc
    45241 cagccaggca gcgttagtgt caacgatttg cccgtgtaac acttcatccc cggtggatag
    45301 catttccact tttaacatgg ttatctcctg cttttgttat cgaatcacta taacgcaaag
    45361 ggagataagg cgtagtaaga aactgaatgg cgcgacagaa tgccgcgccg gaagggatca
    45421 gaaactggcg ttaaccccaa catacgggcc gtcagccacg gcgttgtcgc ggttaccgcc
    45481 tttacccgac agattcaggt agcgataacc cgcctcaata ctgactggac gcataatggt
    45541 gtaacgcgcg ccagcattcg cttcctcata actttgaata ccgctggaga gcgaatccgg
    45601 agagtagtaa tactcgccaa acaaacggaa gctgttgcca attttccact gcaaaccacc
    45661 tcctaccgct gcggcataac cttcatcgcc gtaattcggg ttggtgtaaa cgccttttcc
    45721 gccaacggtc gccattaacg gcccgagagg cagattcaac cccagcccca cgcccgcgac
    45781 gtcaccgtcg tcgtcgttat gtgtccagtt accgcttaaa gccaggcccg tcgattcagt
    45841 cccaaaaccg acaccaatat tggtgtattc tttacccgcc tggccgctga tgctcattgc
    45901 attgaccgat gcagaaacca acagcattcc ggccagacct gctagcgcaa tttttttcat
    45961 tatccccttt cccacaagca attaatcaaa attcccaaag ctgcgggatt gtactgcctg
    46021 gcacagagga ttcaatgagc gaaaaaaggg atctagtcac ttattgtaac taaatacttc
    46081 ttaaataatt aaacgccgaa aaaataaaca cactttaccg atacacacaa aaataataat
    46141 aagtgcagtt atcattgcaa aagagattac tgtcactttc acatatttct gagttaccgt
    46201 attctcatgg tttcttttta tttataagga atacccctat gcgacaacgg actattgtat
    46261 gccctttgat tcaaaatgat ggtgcttatt tgctgtgtaa aatggccgac gatcgcggcg
    46321 ttttccccgg tcaatgggcg ctttcgggtg gcggcgtgga gtctggcgaa cgaattgaag
    46381 aggcactacg ccgcgaaatt cgcgaagaac tgggagaaca gctgcttttg acagaaatca
    46441 tgccgtggac cttcagcgat gatattcgca ccaagacgta tgcagatggt cgcaaggaag
    46501 agatttatat gatttacctg atttttgact gcgtttctgc caaccgagaa gtgaaaataa
    46561 acgaagagtt tcaggactac gcgtgggtaa aacctgaaga tctggtgcat tatgatttaa
    46621 atgttgccgc ccgaaaaacg ttacgtttga aaggtcttct ttaacaatga tgacagccat
    46681 cactggctgt cagattgaaa atttagtagt taacgaattc cccatccaga taaactttgc
    46741 ctttttccac atgcatgact aaaccatcca gataatcagg tttaaatgtc gcatcacgct
    46801 tatttttagc aatatatgtc aggcaatgat tatgggtgaa aataacgata tttttatcag
    46861 gcgctttgct ttgtaagtcc ttaattgcac tataaatctc attaccgcac tgaagaagtc
    46921 gtttatctac cgtcagcttt ttacccgcgg aaaaccaggt cgccgactga atggtccgga
    46981 cggtattact ggaataaaga tcgaaatcag ggatgtcagc gctaaaagcg ttgcccagtt
    47041 cacgggcatc ctgggtacct ttaaccgtaa tacctgtttt atctgacaag cattgattgg
    47101 ttgaacgatc gcaacgttca gcatggcgaa acaaaacgac aaccgggtgc tgctgtgcca
    47161 gtctggccaa tgttttattg tcgatacgtg gcaaaccatt gctactccag gcggcatgag
    47221 tacccagtcc ggcaattgca gcgagcgcca gtaaaatgat gaaatatttt tttgacttca
    47281 atgatgagcg gcaaaaagct aacacgtaaa ctccacctat agacaagcgc aaccagacaa
    47341 ttaccgtaaa attgagcgac atttctggcg ataattagca gttggtgtaa tattaaaaat
    47401 cctacgatgt cggcaaaatg cctcaaaatt ttgccaaatg caaagcctaa ataagaaaaa
    47461 atataaaaat ttcaatattt acgtctaata ttagtttctt aaggttaagt taatattcta
    47521 tccttaaaat ttcgctccaa atggcaaaat atacacaaca ctctttatag caaatataag
    47581 tggacaggta ttcaatggcg gaaggaaaag caatgtcaga atttttgcct ttttcgcgac
    47641 cagcaatggg cgtggaggaa ctcgctgcag ttaaagaggt tctcgaatcc ggttggctca
    47701 caaccggtcc gaagaatcag gcgcttgagc aagccttttg ccagttgacg ggaaatcagc
    47761 atgccatcgc ggtcagttca gccaccgccg gaatgcatat cacgctaatg gcgttggaaa
    47821 ttggcaaggg cgatgaagtg attacgcctt ccctgacctg ggtttcaacc ctcaatatga
    47881 tttccttgtt gggtgcaacg ccggtaatgg tggatgtcga ccgcgatacg ctgatggtca
    47941 cgcctgaagc gatcgagtca gccataacgc cacgcactaa agccatcatt ccggtgcatt
    48001 atgccggtgc gccagcagat attgacgcca ttcgcgccat tggcgaacgt tacggcatcg
    48061 cagttatcga agatgctgcc catgccgtcg gtacgtatta caaagggcga catattggcg
    48121 caaaaggtac cgctattttt tcatttcatg ccattaaaaa tattacctgt gcggaaggtg
    48181 gcctgattgt aactgataat gaaaaccttg cccgccagct acggatgctg aaatttcacg
    48241 gtctgggtgt cgatgcctat gacagacaaa cctggggccg tgcaccgcag gctgaagtct
    48301 taacaccggg ctataagtac aatctgaccg atattaacgc cgcaattgcc ctgacacagt
    48361 tagcaaaatt agagcacctc aacacccgtc ggcgcgaaat tgcccagcaa tatcagcaag
    48421 cactggcagc tctccccttt cagccattaa gccttcccgc ctggccgcac gttcacgcct
    48481 ggcatctgtt tattattcgt gtcgatgaac aacgttgtgg tatcagtcgc gatgcgttga
    48541 tggaagcgtt aaaagaaaga ggcattggta ccgggttaca tttccgcgcc gctcacacac
    48601 aaaaatatta tcgcgagcgt tttcccacgc tgtcgttacc gaataccgaa tggaatagcg
    48661 aacgcatctg ttctttgccg ctgttcccgg atatgactac cgccgatgcc gaccgtgtca
    48721 tcacagccct tcagcaactc gcaggacaat aagccatgtt tgaaatccac cctgttaaga
    48781 aagtctcggt ggttattccc gtttataacg agcaggaaag cttaccggaa ttaatcaggc
    48841 gcaccaccac agcctgtgaa tcgttgggga aagagtatga gatcctgctg attgatgacg
    48901 gcagtagcga taattccgcg catatgctgg tcgaagcctc acaagcggag aacagccata
    48961 ttgtgtctat tttgcttaac cgcaattacg ggcaacattc agcgattatg gcgggattca
    49021 gtcacgttac cggcgactta attattaccc ttgatgccga tctccagaat ccgccagaag
    49081 aaatcccccg cctggtggca aaagccgatg aaggttacga cgtggtaggg actgtacgcc
    49141 agaaccgcca ggacagctgg tttcgtaaaa cagcttcgaa gatgattaac cggcttattc
    49201 agcgcaccac cggcaaagcg atgggtgact acggttgtat gctgcgcgcc tatcgccgtc
    49261 atattgtcga tgcgatgttg cactgccatg aacgtagcac ctttatcccg attctggcga
    49321 atatcttcgc ccgccgtgcc attgaaattc cagtacatca ttccgagcgt gagtttggtg
    49381 aatccaaata cagttttatg cgcctgatta atttgatgta cgacctggtg acctgcctta
    49441 ccacaacgcc gctacgtatg ctgagtctgc tcggcagcat tattgcgatt ggaggtttta
    49501 gcattgcggt gctgctggtg attttacgcc tgaccttcgg accacaatgg gcggcagaag
    49561 gcgtctttat gctatttgcc gtgctgttta cttttattgg cgctcagttt atcggcatgg
    49621 gattactcgg tgaatatatc ggcaggatct acaccgatgt ccgcgcccgc ccccgctatt
    49681 ttgttcagca agttatccgt ccatccagca aggaaaatga ataatgaaaa ccgtcgtttt
    49741 tgcctaccac gatatgggat gcctcggtat tgaagctctg ctggctgccg gttacgaaat
    49801 tagcgccatt tttacccata ccgataatcc cggtgaaaaa gccttttatg gttcggtggc
    49861 tcgtctggca gcggaaagag gcattccggt ttatgcgccg gatgacgtta atcatccgct
    49921 gtgggtggaa cgcattgccc aactgtcgcc agatgtgatt ttctcttttt attatcgcca
    49981 tcttatttgc gacgaaattt tgcagctcgc tcccgcaggt gcatttaatc tgcatggttc
    50041 gctgttacca aaatatcgtg gtcgcgcgcc gctgaactgg gtgctggtca acggtgaaac
    50101 ggaaactggc gttacattgc accgaatggt gaaacgtgcc gatgccgggg ccattgtggc
    50161 gcaactgcgc attgccattg cgccagacga tatcgctatt acgctgcatc ataaattgtg
    50221 ccatgccgcg cgccagctac tggaacagac attacccgcc attaaacacg gtaatattct
    50281 ggaaatcgcc cagcgcgaaa acgaagccac ctgttttggt cgcagaacgc cggatgacag
    50341 tttccttgaa tggcataaac cggcatccgt actgcacaac atggtacgtg ccgttgccga
    50401 tccgtggccg ggtgccttca gctatgttgg caatcagaaa ttcaccgtct ggtcgtcgcg
    50461 tgttcatcct catgccagca aagcacagcc ggggagcgtg atttctgttg cgccactgct
    50521 gattgcctgt ggcgatggcg cgctggaaat cgtcaccgga caggcgggcg acggcattac
    50581 tatgcagggc tcgcaattag cgcagacgct gggcctggtg caaggttcac gcttgaatag
    50641 ccagcctgcc tgcaccgccc gacgccgtac ccgggtactc atcctcgggg tgaatggctt
    50701 tattggcaac catctgacag aacgcctgct gcgcgaagat cattatgaag tttacggtct
    50761 ggatattggc agcgatgcga taagccgttt tctgaatcat ccgcattttc actttgttga
    50821 aggcgatatc agtattcatt ccgaatggat tgagtatcat gtcaaaaaat gtgatgtcgt
    50881 cttgccgctg gtggcgatag ccacgccgat tgaatatacc cgcaacccgc tgcgcgtatt
    50941 tgaactcgat tttgaagaga atctgcgcat tatccgctac tgcgtgaagt accgtaagcg
    51001 aatcatcttc ccgtcaactt cagaagttta tgggatgtgt agcgataaat acttcgatga
    51061 ggaccattct aatttaatcg tcggcccggt gaataaacca cgctggattt attcggtatc
    51121 aaaacaatta cttgatcggg tgatctgggc ctatggcgaa aaagagggtt tacagttcac
    51181 cctcttccgc ccgtttaact ggatgggacc gcgactggat aaccttaatg cggcacgaat
    51241 cggcagctcc cgcgctatta cgcaactcat tctcaatctg gtagaaggtt caccgattaa
    51301 gatgattgat ggcggaaaac aaaaacgctg ctttactgat attcgcgatg gtatcgaggc
    51361 gttataccgc attatcgaaa atgcgggaaa tcgctgcgac ggtgaaatta tcaacattgg
    51421 caatcctgag aacgaagcga gcattgagga actgggcgag atgctgctgg cgagcttcga
    51481 aaaacatccg ctgcgccatc atttcccacc gtttgcgggc tttcgcgttg tcgaaagtag
    51541 cagctactac ggcaaaggat atcaggacgt agagcatcgt aaaccgagca tccgcaatgc
    51601 ccaccgctgc ctggactggg agccgaaaat tgatatgcag gaaaccatcg acgaaacgct
    51661 ggatttcttc ctgcgcaccg ttgatcttac ggataaacca tcatgaccaa agtaggctta
    51721 cgcattgatg tcgatacctt tcgtggcacc cgtgaaggcg tgccgcgtct gctggaaatc
    51781 ttgagtaagc ataatattca ggccagcatt tttttcagcg tcggcccgga caatatgggc
    51841 cgccatctct ggcgactggt gaagccacag tttttgtgga agatgctgcg ctcaaatgcg
    51901 gcatcgcttt atggctggga tattttactg gcaggtacgg cctggccagg taaagagatt
    51961 ggtcatgcca atgccgatat cattcgtgaa gcggctaaac atcacgaagt cggcctgcac
    52021 gcctgggatc accatgcctg gcaagcccgt agcggtaact gggatcggca aacaatgatc
    52081 gacgatattg cacgcggtct tcgcactctg gaagagatta tcggtcaacc ggtaacctgt
    52141 tctgccgctg cgggctggcg tgccgaccag aaggtgatcg aagcaaaaga agcgttccat
    52201 ttgcgctaca acagcgattg tcgtggggcc atgccgttcc gtccattgct cgaatcagga
    52261 aaccctggca ctgcgcaaat tccggtgacc ttacccacct gggatgaagt gattggtcgg
    52321 gatgtgaaag cagaagattt taacggttgg ttactcaacc gcatcctgcg agataaaggc
    52381 acgccggttt ataccattca tgcagaagtt gaaggctgcg cttatcagca taattttgtg
    52441 gatctcctca aacgcgcagc tcaggaaggc gtgacatttt gccctttaag cgaactgtta
    52501 tcagagacgt tgccgctcgg acaagttgtt cgcggaaata ttgccggacg tgaaggctgg
    52561 ctgggttgcc aacaaattgc gggtagtcgc tgatgaaatc ggtacgttac cttatcggcc
    52621 tcttcgcgtt tattgcctgc tattacctgt taccgatcag cacgcgtctg ctctggcaac
    52681 ccgatgaaac gcgttatgcg gaaatcagtc gagaaatgct ggcatccggc gactggattg
    52741 tgccccaact gttagggcta cgttatttcg aaaaacccat tgccggatac tggattaaca
    52801 gcattgggca atggctattt ggcgcgaata actttggtgt gcgggcaggc gttatctttg
    52861 cgaccctgtt aactgccgcg ctggtgacct ggtttactct gcgcttatgg cgcgataaac
    52921 gtctggctct actcgccaca gtaatttatc tctcattgtt tattgtctat gccatcggca
    52981 cttatgccgt gctcgatccg tttattgcat tctggctggt ggcgggaatg tgcagcttct
    53041 ggctggcaat gcaggcacag acgtggaaag gcaaaagcgc aggattttta ctgctgggaa
    53101 tcacctgcgg catgggggtg atgaccaaag gttttctcgc ccttgccgtg ccggtattaa
    53161 gcgtgctgcc atgggtagcg acgcaaaaac gctggaaaga tctctttatt tacggctggc
    53221 tggcggttat cagttgcgta ctgacggttc tcccttgggg actggcgata gcgcagcggg
    53281 agcctgactt ctggcactat tttttctggg ttgagcatat tcaacgcttt gcactggatg
    53341 atgcccaaca tagagctccg ttctggtact acgtgccggt catcattgcc ggtagcctgc
    53401 cgtggctggg attactcccc ggtgcactgt acacaggctg gaaaaaccgc aagcattccg
    53461 caaccgtcta tttgttgagc tggacgataa tgccgctgct gtttttctcc gtcgctaaag
    53521 gtaaattgcc cacctatatt ctttcctgct ttgcatctct ggcaatgctg atggcgcatt
    53581 acgctttgct ggcagcaaaa aataatcctc tggcgctgcg gattaatggc tggattaaca
    53641 tcgcttttgg cgtcactggc attattgcca catttgtggt ctccccgtgg ggaccaatga
    53701 acacgccggt gtggcaaacc ttcgagagct ataaagtctt ttgtgcctgg tcgatttttt
    53761 cgctatgggc atttttcggc tggtacacct taacaaacgt cgaaaagacc tggccttttg
    53821 ccgcgctttg cccgctgggg ctggcgttgc tggtaggatt ttcaattcct gacagagtta
    53881 tggaaggaaa acatccgcaa ttttttgtcg agatgacaca agaatcactt cagccaagcc
    53941 gctatattct tactgatagc gtcggtgttg ccgcaggtct ggcatggagc ctgcaacgcg
    54001 atgacatcat catgtatcgc cagacaggtg agttgaaata cggccttaat tatccggatg
    54061 cgaaagggag atttgtcagc ggtgatgagt tcgcaaactg gcttaatcaa catcgtcagg
    54121 aggggattat tactctcgtg ctttcggttg accgcgatga agatatcaac agtctcgcca
    54181 ttccgcccgc agatgccatc gatcgtcagg agcgtctggt gctgattcag tatcgtccca
    54241 aatgatctgg ctaacattag tctttgccag cttgcttagc gttgccgggc agttgtgtca
    54301 gaaacaggca acctgctttg tggcgataaa caaacggcgc aaacatatcg tgctgtggct
    54361 gggactggcg ctggcttgtc ttggtcttgc catggtgctc tggctgctgg tcttgcagaa
    54421 cgtaccggta ggcattgcct acccgatgtt aagtctgaat tttgtctggg tgacgctggc
    54481 tgcagtaaaa ctgtggcacg aaccggtatc gccgcgtcac tggtgtgggg tggcgttcat
    54541 tattggtggc attgtgatcc tcgggagtac ggtgtaatgg gcctgatgtg gggattattc
    54601 agcgtcatta ttgcctcagt tgcgcaatta agcctgggtt ttgcggcgag tcatctgccg
    54661 ccgatgacgc acttatggga ttttatcgcg gctctgttag cttttggcct ggatgcccgg
    54721 atactgttac tcgggctgct gggttatctg ctttccgttt tttgttggta taaaacgcta
    54781 cataaacttg cgctaagcaa agcctatgcc ttgctcagta tgagttatgt gctggtatgg
    54841 attgcttcaa tggtacttcc cggctgggaa gggacttttt cgctgaaagc actactggga
    54901 gtagcctgta ttatgagcgg gttgatgctg attttcctgc ccatgacaaa acaacgttac
    54961 tgagttttcc ctgccacttt acactgccgt tcccactcat cggggctata actgcttgcc
    55021 gagaggactt tcaccgtatg cagcttttca cgattaatac aataaagcgc attctgcaaa
    55081 ggcgagagta aatcccccac tttcacggcg aagtcactct tcacttcggc gatcattttt
    55141 atcgcgccgc cagcatcgca gagcataagt acatgtctgt tatcttgttt attgcagcac
    55201 gattttttga ccagccattc cattgcatta tcctgtttgc taagagtttt cacatcaatt
    55261 tacagattaa cagataaact ctttatcaac ggcaatggaa tggcagaaat gagaagtgaa
    55321 acggagggtt aattattgct gacgttgcac ccactctttt agcgcctgac gtgaaatttt
    55381 aataccgccg tttttcagtt caggcggcaa cgttaaccaa cgcaccggtt gctgaaaact
    55441 ggccagctta tctttcaccc attcactaag atcaacgctc tcgtggtcat actccatcac
    55501 cgccaccggt cgatgaccaa actccttgtc ggcaacgggg acgataaaca cctgcaaaac
    55561 cgcaggatgt gcagcaatta cgcgctcgac ttcttccggc tgaataccct caccgccact
    55621 gaagaacaga ttgtctaaac gtccgacaat ggtcagcttg ccattatgca tctcaccgcg
    55681 atcgcgcgta gcgtaccagc cttcgtcatt aaccagtgaa accagttgcc cgttacgcca
    55741 gtaaccttct gccatactgg cagcccgcag ccacacttca ttattaacga ttttcacttc
    55801 ccgacccggc agcggcgaac caacgtctgc caggccgtcg gcttctttcg cacacaccgt
    55861 ggaggcaaac tcggtcagac catagccgca aaagcaacga atcccctgct cgcgcgcctg
    55921 ttccgtcaac tcgaccggga tagccgcgcc gccaagtaac accgctttca gggaaacgga
    55981 actgcggtta accagcaaac gccagagttg tgttggcacc agtgaagcgt gagtacagcc
    56041 tgccagcatt tgctccaatg gctgtttatc acgcaccgtc atccgcgcac cagcgtataa
    56101 ccagcgccac ataattccct gaccggagac gtgaaacagc ggtaaagaga gcaaccagtc
    56161 atcgtgatcg ccaaacggaa tcaacgataa caccccttcg gcactggcaa gatgggcctg
    56221 ataagtatgg acagcggctt tcggcaaccc ggtagaaccc gaggtcaacg tcattgagca
    56281 cagacgcgtc ggctgccacg tagcggcatg tgcgccttca acccgctgaa tgtgcagcga
    56341 cgttaacgcc ggaaacgtgt tttccccatc cggcaccagc gcaaattgca gcgtcagatt
    56401 gggcagcaat tcttcaagca acggttgcgg cagctgaggg ttcacgggca acacccgcgc
    56461 cccgcattgc agtaacgcca gccaggcgag cagcgtttgc ggcgtattcc acgcccgcaa
    56521 catcacgccg ctgccctcaa caacgccctg cgccgcaaag cctgaggcta attcatcgac
    56581 gcgagcacaa agctcgcgcc agttaagttg ctcgtcatta agacgtaagg cgatggcttc
    56641 tccccgcact tgccgccagt gacgccacgg ccagtcagag aagatcataa caaccgctcc
    56701 agtgcatcaa cttccacgac aggcagcgtg ctacccggcc agcgacgtac ctgctgcgcc
    56761 tgcatcagat ccagcgtgtc cagccctgga atggtgtccg gcgttaacca ggcggcaatc
    56821 cgcgccagtt gcgttaagcc taagctcgat tcaatggaag aactgatcac cgccgtcagc
    56881 cccagcgcgt gcgccgcctg tacctgctcg cgtactttct ccagactgcc cgtgagcgtg
    56941 ggtttgataa ctaccgcgcg cacgccctct tcagccacaa aggcaaaatc cggctcgcgc
    57001 aagctttcat cccaggcaat ggcaatgccg gtttcacggg caaacgctcg cgaatcatcg
    57061 cgggttttgc acggctcttc gagaaacgcg atgcggtggc gataatccgg gttaacatat
    57121 ttggcaaact gctggccttt cagcggtgtc caggcgcgat ttgcgtcaag acgcaaatgc
    57181 agatccggaa ttgcctccag caacagatta accaccatgc cgtcgcgcac ggcttcgtac
    57241 aatcccactt tgaccttcgc cactttctcg cctggcatat cagcaagttt gaggatcaga
    57301 tcgtcaggat cgccattaca cagtggtgcc gcacggtaat tggccgcctg cgggagcgta
    57361 tctgccagct ccgccaatgc acagcttacg ccaaaggcaa cggaaggcat ctgcggtagc
    57421 tcgcaatcgc ctgccagcca gttatttacc caggcaagca gcacgctttg cgcctcttcc
    57481 caggtttcct ggctgaagcc cggcagtggg gagatctccc cccacccttc gcgctcgccc
    57541 tcacgcaggc aaacgtacag cccgtcgcgg gtttttaacc gcctgtcgcg cagaaccacc
    57601 cccgcgtcca tggggatctg ccagcggtat acctgcgcgc tacgcattac ggattccgtt
    57661 taaatttgct gaaatcaggc tgacgtttct ggttgaaagc gttgcgacct tcctgacctt
    57721 cttccgtcat gtagaacagc atggtggcgt tgcccgccag ctcctgcagc cccgcctgcc
    57781 cgtcacagtc ggcgttcagc gcagctttca ggcagcgcag cgccatcggg ctgttttgca
    57841 gcatttcgcg gcaccaacgg acggtttctt tttccagatc cgccagcggt accacggtgt
    57901 tgacaaggcc catatccagc gcctgttttg cgtcgtactg acggcacagg aaccagattt
    57961 ctcgcgcttt tttctgcccg acgatacgag ccatgtagga agcgccccag ccgccgtcga
    58021 aggaaccgac tttcgggcca gtctgaccga agatggcatt atctgctgcg atagtcaggt
    58081 cgcacatcat gtgcagaacg tgaccaccgc cgatggagta gccagccacc atcgcgacaa
    58141 ccggtttcgg acaggtacgg atctgacgct ggaagtccag cacgttcaag tgatgtacgc
    58201 cggaatcatc tttatagccg ccgtaatcac cacgcacttt ctggtcacca ccggagcaga
    58261 acgctttatc gcctgcgcca gtcagaatga tcacgccgat attgtcgtca taacgcgcat
    58321 ctgccagtgc ctggatcatc tcttttaccg tcagaggacg gaaggcattg cgcacctgcg
    58381 gacgattaat ggtgattttt gcgataccgt cggtggattt ttcataacga atgtcctcga
    58441 aaccttcgga gcagtcgtgc cattcaaccg gtgcgtaaag cattgcttca tcaggataaa
    58501 tcatattgtg tcctttagtc agaaacgcaa gatctgcgcc agacttgcga ttacgccagc
    58561 gggattttcc cgatgcgcgt tatgtccggc gcgaggaatg acatggcagt cggcagccag
    58621 ttccgccgcc agggcgcgga atttgctgtc acgttcacca cataaataat aaaacgcaaa
    58681 tgtgcgggcg ctaaggttag cacgtaaatc aggctggacg gcgagagaag tcgcctccag
    58741 catggcggca agggttgcgc cattattgtt gctgcgcagc gccaccagct cccggcgttg
    58801 atcgtcattg agcgaggcaa aaacaggctg ttgataccag tcggcaaata ccgccgttaa
    58861 cggttctgtg cgaaaacgct gcgcccattg gcgatcggaa cgctgacgtt ccgcacgttg
    58921 ttcagcattt tgcagccccg gatgcccgcc ttcgacaata accccacaaa gccccgccag
    58981 cccctggcaa gccgccatca tcgccacccg tccgccaagc gagtacccca ctagccaaaa
    59041 gtcaaggatg ttgtaactaa ccaaggtttt acgcagtaag tcggtgacat catcaaatcc
    59101 atcgacgcta atcgccgccg aaccaccatg acctgggaga tcaacataca accgtgagta
    59161 gtcggcaaac gcctcgccca cttcttgcca ttcgtggcaa tcgccggaaa aaccgtgcaa
    59221 aaacaccagc cagggtaaac ctggttttcc gtgttttgcc tgcgcgtgca ggatcataaa
    59281 tggcttacct gcgccagaag ttgctggagc gtttgcgcac catcggtgtc gttaaccacc
    59341 atttcaatca ccgtggtggt tggcgtgcgc caggcgtcgg caaatgccgt ttcaagttcc
    59401 tgccagtttt gcggacgatg atatttcagc tcgaacatcg cggcggcgtg ctcaaaatgg
    59461 acgttttgcg gcatcagata gaaacgctca cgctcgcttt gcggcgttgg caacagcgag
    59521 aaaatttgcc cgccgttgtt gttcaccaca attaatacca gcggcgcaga aacctgacgc
    59581 aataacgcca gcgcgttgag atcgtaaagt gcggagagat cgcccacaat cgccagcgtc
    59641 ggtttgccgc ttgcccgctg aacgcctgcg gcggtcgaaa gcagtccgtc gataccgctg
    59701 gcccctcggt tgctgtacac cgggtaacct gctggaagtt gcgaaagcgc atcaatcaga
    59761 cgtaccacca ggctgttacc aacaaacaat tgcccctgtt caggcagata gtcgcagatg
    59821 cgatgcgcca gttgcgcttc gccaaacgca tcacggcggg caataaccgc ctgcattgcc
    59881 tgttccgcca ggcgcgggat ttcaacgcac cagggctggc gtttttctgc cggatgcagc
    59941 tccagccagt cggcaatatt ggcaattaag cgacgtccgc gatggtgtgc cggatcaagt
    60001 cgcccttcaa tgtcatcaac aatccagtac tcttctggtt cacagcttgc ctgccattgc
    60061 aggagccgtt tgcccgtcag gctgcttccc agttgcacca caatttgcgc ctgctgcagc
    60121 tcgctggtcg ctttggcatt gcctaaccaa agatcggcac acggcagcgg ctgcccggtt
    60181 tgtgacagca catcgccaat cagcggccag ccaagagttt gcgcccacag ggcaactttt
    60241 ttgccctctt ccgcactcat gcgcccggca accaccacgc cgcgcttttg tcgccagaag
    60301 aaccagtcgc gctgtttttc actttccaga cgaggcgctt cacgcagcca cggtttgtcg
    60361 tcctgccacc agtcacccag acgctgttgc cagctaagcc cggtatcgtc catttcgcca
    60421 tacagcggtt cagcaaacgg gcagttgata tggactcccc ccgcatgaag cgtaccgaga
    60481 gcgtggtcga tggtagaaac cagccaacgt gcggggatat cccgggtcgg gcgcggcaat
    60541 gaaatactgt gcgtggggtg agaggcgaac attcccggct ggcgaattgc ctgattcgcg
    60601 ccgcagtcaa ttagctccgg cgggcgatcg gcggttaaca ggatcagttt ttctccggtt
    60661 aacccggctt caatcagtgc aggatagaga tttgctaccg ccgtgccgga ggtcacaatc
    60721 accgccaccg gctgcttgct gactttcgcc agccccagcg ccagatgccc caacccacgc
    60781 tcatcgaaat gggtgtggtg aatgaatgcg gaattctccg ccgccgctaa cgttaacggt
    60841 gtagaacgcg agccaggggc gatacagatg tgtctgacgc cgtgacgcgt taatgcttcc
    60901 agaatgaccg ccgcccagcg tcggttaaat gcgcttactg acatgagttt gtccggtatc
    60961 aatattgcgg ctaagtataa ggagctacaa aaatagatta ttgatatgaa tcggtaatga
    61021 tgcgactcat tactattcca tttgtaataa agtacgcagc cctgccgctt tgttgtcgat
    61081 ttcctgccac tcttgctcgg ggtcggaacc acggacaatg cccgcgccag catatagccg
    61141 cacgacattg ccgctgattt ttgctgaacg cagggaaacg cagaattcgc tttgttgtaa
    61201 tgagagatag cccgcagaac cggcgtacca ttctcgggtg aacggttcgt gacgggcgat
    61261 aaactgtcgc gccagatcgc gcggtaagcc agcaactgct gccgttggct gcaactgatg
    61321 taaacagatc acatcatccg ctttgttgag tgaagtccag atacagcggc gaagatgctg
    61381 cactttacgc agacgcagta cctgcggcgg taaaacatcc agcgtctggg tatcggcctg
    61441 taatcgttgg cagatatctt ccaccaccag catgttctcg cgctggtttt tatcatccgc
    61501 catcagccac tctcctaact gctgcgcctg cttatcatca ggattatttg ctactgttcc
    61561 cgccagcgct tcagtacgca gcgctttgtc acgccgccgc cataaccgtt ccggtgaaga
    61621 gccaagaaaa gcattttcgc catcaaaggc catgtaaaaa tggtagcaat tcagattcag
    61681 tcgacgactg gcagccatca tcgccgccgc gttgaccgga cttgcgaaat gcaggtcagt
    61741 tgcccgagcg agcaccactt tgtcgagctc cccttcggcg atggttttcg ttgccagttc
    61801 gattaattgc gtccagcccg ttttgtccgg ccagtgttgt tctcgcgtgg tggttaaatg
    61861 taacccaggc aagggcttga tactcaccag tgtggcgata aattctcttg cctgaatcgc
    61921 atcgtgctga agggagcttt cgctgaatag cgtcagccgc agcgtggctt taccgccaca
    61981 gcgtcgccat tccaggcgtg gtaaaagtaa attgccctgc gacgggtcaa acgcattcag
    62041 cccccaaatg cgtaagtcgg cgtgttccgg gtgctggcga agaaaacgtt gtgcctggtc
    62101 caacgacgta aaacgggtaa tcgcgcccag gacggcagct tcttcatcac cattacgttg
    62161 ttgccagtag aattgcgggt acgtttgctg actggccagc cagctcaagg catcaaaagc
    62221 gtctttgaga gggaaaggaa tatcgataac ccgaatgccg ggtgtcgccg gaatctcttg
    62281 cgacaaatgg cgcagtagat tttccagcgc cgtagtaagt gattgcacgc ggacctctcc
    62341 ctgttaaaag cctcacatta tacggggtac tacaaaaaaa atgcagtacc ccggtgtagg
    62401 gaggttttaa cggcgtgcca gcaacagtcc tagtaccagc ccgacggccg cgcccacacc
    62461 aattccttgc cagggttttt cgtggacgta gtcatcagca cgataaacag cctgcttcgc
    62521 ccgatagtaa taactgtctg aagcctggct aacccgtttt ttcacatcat ccagcgcttt
    62581 ttctgcacgc gctttcagct caacatattt ctgatcggcg ggatcgccag aggagcggag
    62641 cacctcttcc agtgtttcac taagcagcgt caggtcgtca tcgatacgtg tatcaccaaa
    62701 ctgattagac attctcgttc tccatttgcg taaaacctgt cactaactat agacaagggt
    62761 tgttgattac gcctgaatta cctcgcgtgc catcccaatg tgtgggatac catcttcttc
    62821 atagacctcc gtcacaggga taaaaccgaa actctggtag aagttttgca gatgcgcctg
    62881 cgccccgagg taaacaggct tatcaggcca gtgatgcgtg caggtgtcga gggttttact
    62941 catcagttgc tgccccactt tttcgccacg caacgcttca ctgacaatca cccgacctat
    63001 aacgaccggc tcaagatcat catcactttt cagaatcctc gcatacgcca ccagttcatc
    63061 atttttccaa ccgagaatat ggcgattatc ccccgtcaaa tcatcaccat cgatatcctg
    63121 ataagggcaa ttctgctcga caacaaacac cgcgcaacgc agttgtaata aggcataaag
    63181 ctgggaaacc gataattcag agtgatgcag atcttgccat tcaatcataa tgctctcctt
    63241 ttagatgtat gccttatact agccggaata ttttttgaaa cgtgatgaac tcatggaatt
    63301 aattttttta ggtacttcag ccggtgtgcc aacccgcacg cgcaatgtca cggcaatatt
    63361 gcttaacctg caacatccga cccagagcgg actttggttg tttgactgcg gtgaaggcac
    63421 ccagcatcag ctactgcata ccgcctttaa ccctggaaaa ctggacaaga tttttatcag
    63481 tcaccttcat ggcgatcatc tttttggttt acccggcttg ctgtgcagtc gttctatgtc
    63541 aggcattatc caacccttaa cgatttatgg tccgcaaggt atccgtgaat ttgtggaaac
    63601 cgcgctgcgg attagcggct catggaccga ttatccgctg gaaattgtcg aaattggcgc
    63661 tggcgaaatt ctcgatgatg gcctgcgcaa agtaaccgct tatccgctgg aacacccact
    63721 ggaatgttat ggctatcgta ttgaagaaca tgataaaccg ggtgcattaa atgcccaggc
    63781 attaaaagct gctggcgtgc cgcctggccc actgtttcag gaattaaaag cgggcaaaac
    63841 aatcacgctg gaagatggaa ggcagattaa cggcgcagat tacttagctg ctccagtgcc
    63901 aggtaaagcg ctcgctattt tcggcgatac cggcccctgc gatgccgcac ttgacctggc
    63961 taaaggtgtc gatgtcatgg tgcacgaagc gacgctggat ataaccatgg aagccaaagc
    64021 caatagtcgc ggccatagct ctacacgcca ggctgcgaca ctagcccgtg aggctggagt
    64081 cggtaagcta atcattaccc acgtcagctc gcgctatgat gacaaaggtt gtcagcacct
    64141 gttacgtgaa tgcaggtcaa ttttcccggc gactgaacta gcgaatgatt tcaccgtgtt
    64201 taacgtttaa ctctgcttat tattaaaaac agggcgaaac ttgccctgtt atcgcaaccc
    64261 gcgctacata tatccaccca cacgggcata ttgagcgatt tttgatattt attcatctcc
    64321 ttacgcacta tattcagagc aataagttgc ctctttttat cttcattcag cacaaccttt
    64381 gcaactaaaa ggagcccatg atggttacag ttgtcagcaa ttattgtcaa ttatctcaaa
    64441 cccaactcag tcagacattt gcagaaaaat ttactgtgac cgaggaatta ctgcagtctt
    64501 taaaaaaaac agcgttatcc ggagatgaag aaagtattga gttactgcat aatattgcgt
    64561 taggttatga tgaatttgga aaaaaagctg aagatattct ttaccatatt gttagaaacc
    64621 caacaaatga gaccctatcg attatccgac ttattaaaaa tgcctgttta aaattatata
    64681 atttggcaca taccgcaacc aactcccacc ttaaaccaac tggtccggat aattcagatg
    64741 ttctcctgtt caaaaagcta ttctcccctt cgaaattaat gacaattatc ggtgatgaaa
    64801 ttcctcttat atctgaaaaa cagtcgcttt caaaggtgct tttaaatgat gagaataatg
    64861 aactgagtga tgatacaaac ttctgggata aaaatcgtca attaaccaca gatgaaatag
    64921 cttgctatct tcagaagatc gccgctaatg caaaaaatac tcaagtcaat tatcctactg
    64981 gtctctacgt cccctactcc accagaactc acctggaaga cgctctcaat gaaaatatta
    65041 agagcgatcc atcatggccg aatgaagtcc agttattccc cataaatact ggcggacact
    65101 ggatattagt ttcgctacag aaaatagtaa ataaaaaaaa taataaacta caaataaaat
    65161 gcgtcatatt caactcattg cgtgcactag gccatgataa agaaaattca cttacgcgtg
    65221 tcattaatag ttttaattct gaactcatgg gagaaatgtc gaataataat ataaaagttc
    65281 atttaaatga accagagata atatttttac atgccgatct tcagcaatac ttaagccaaa
    65341 gttgcggtgc gtttgtgtgc atggcagccc aggaagtgat tgaacaaagg gaaagcaatt
    65401 ctgacagcgc cccctatacg ttattaaaaa actatgctga cagatttaaa aaatattcag
    65461 cagaagagca gtacgaaatt gattttcaac atcgactggt aaacagaaat tgttatttag
    65521 ataaatatgg cgatgcacga atcaatgctt cttatacaca attagaaata aaacactcac
    65581 aacccaaaaa tagagcatcc ggcaaaagag tgagttaaca tcattgttaa ttaattcgac
    65641 cagtcagcaa atatgctgac tggtcgaaca tagtttgcta aacagtcatc actgactgat
    65701 gtcagtcaca ccatccgcca gttcaatctt cgggtatttc cagcgtatct cggcacgcga
    65761 ttgctggtct gttgatgtag tggattgttg ttgctgactt ttcttaccct cagagttctg
    65821 ccccctgtca actcaatctg ccaataaata acataattat atttttcctt atatgaattc
    65881 ctttttaaaa tagcgaagca actgtaaaag atactttttg tatagatcta acaaccgtaa
    65941 taaaacaaac actcataact cattataaat aatgataaaa taacgtcttt tattgataat
    66001 ttatatatga aaaaaaatcc ttttgttata ttatttctga tttcactccc tctgattatt
    66061 tttaccaacc ctgggtcact gcattatcgc caacgccccg acacgtcagt cctgagcaat
    66121 tagaaaaaac agttcgttat cttacacaaa ctgtgcatcc acgtaatgcc gacaatattg
    66181 ataatctgaa taggtccgta gagtatataa aagagttctt tgtcagtagc ggtgccagag
    66241 ttaccgcgca gggcgtcccc gattggctat tacgactctg cgcctggtag ccaggattac
    66301 ccttacccgc aatgtcctgg ctttatcccg atcgggggga ctttattgcc tggtcggcag
    66361 aatacaggat atcaacgccg ttcgtcaggt aaaaacggca ttgttatcat cccgggattt
    66421 atctgtttat tctatgaatg ccccatggtt tattcccggt attgatttct ctgaccacct
    66481 gaattattgg caacacgata ttcccgccgt aatgattact gacaccgctt tttatcgtaa
    66541 taaacaatac cacttgcccg gtgatacagc agacagattg aattatcaga aaatggctca
    66601 ggtggtggat ggggttataa ctttgttata caacagtaaa taataaaaat ttctcgaggc
    66661 gggtccagaa aaagcgacat gcacatttta ttttccctcg tttattttaa gatgcgaaac
    66721 ataaaattca acggaggaaa aataaatggg aatgattggc tattttgcag aaattgactc
    66781 agaaaaaatt aaccaactgc ttgagtctac tgaaaaaccc ttaatggaca atattcatga
    66841 cactctttca ggattacgcc gactggatat tgataaacgc tgggattttt tacactttgg
    66901 gctgacaggc acctctgctt ttgatcccgc gaagaatgat ccgctgagtc gtgccgtgct
    66961 tggtgaacac agcctcgaag atggcataga tggcttcctg ggattgacct ggaatcagga
    67021 gttagccgcg acaatcgacc gactggaagc tttggatcga agtgagctgc gtaaaaagtt
    67081 ttccattaag cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt
    67141 agaggggcaa ctctttgctt cgatcatgct tgatatggaa aagctgatta gcgcgtatcg
    67201 acgtatgctg cgacagggga atcatgcgct gacggttatt gttgggtgat ggtgtcagag
    67261 cgccaaaaaa aaccgccgaa catgtcggcg gctttctgaa ttacgaactg acagcttaca
    67321 tcagcggcat ggctaaccgc acaatgctaa tcagcggttg tggccataca cccagcacca
    67381 gtaccaacag tgcagagatc agcacaacga taccgcccgc gctgtactgc cagtttgatg
    67441 gtgcatcgcg acccggttgt tccggggcgt gaagatacag gctcaccgcc acgcgcaggt
    67501 agtagtagag gccgattgcc gaaccgacaa ccacggcacc caccagccac cacaagtgtg
    67561 cctggacacc gaccgccagc acgtagaact taccgataaa gcccagcgtc atcgggatac
    67621 cggccagaga cagcatcatc accgtcatca ctgccgcgag gatcggacga tgccagaaca
    67681 gaccgcggta agagaacagg gaatcagcat ccgggccacg atacgggctg gacatcaggc
    67741 tgaccacgcc gaacgcgccg aggctgctga acagataacc ggccaggtaa acccctaccg
    67801 cttccatcga catctcgccg gtttgcagcg caatcagcgc caccagcaga tagccgaggt
    67861 gagagataga tgagtaaccg agcagacgtt tgatgttggt ctggctcaac gccatcaggt
    67921 taccgaagat gatggaggca aaggcgataa tcgccagtac tacgcgaatc gcttcgctgt
    67981 cacccaccgg tgcgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg
    68041 ccgtcgccag gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca
    68101 ggtggaacgg caccagagag agtttgaagc cgaggccaac aatcatcagg ccgaaacctg
    68161 ccagcaacag cggctcgttg agcataccgt cgccgaggtt tttacccaac gcgacaaacg
    68221 acaggtcgcc agactgcgca tacaccagcg ccataccgaa cagcaggaat gaagacgctg
    68281 cggcagaaag gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt
    68341 aaccgaccag gccaaacagc ggcaaagaga tcagttcaat accgaggaac agagacgcca
    68401 gatggttggc attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga
    68461 actcatcctt gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga
    68521 ggctcgccaa caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg
    68581 gcgtaacgtc catagcgccc gcctggccga caaaccagag cgaaaccagc gccgcgttaa
    68641 gcccaataac cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca
    68701 tcacaaccac caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg
    68761 gagttattgt catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct
    68821 ggatattgcc aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca
    68881 gtaccagcag caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca
    68941 gttcctggct ggcaatctgg cttttcgctt taccgaagta agcgcgatgt aacatcgcca
    69001 gcgaataaac agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa
    69061 cctggaagct gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc
    69121 caagcgttgc caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca
    69181 gaccgcccat catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccacaaagaa
    69241 taaacagacc cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg
    69301 ccaactggct gccggtgtag atggcaatca gcacgaagcc catgtgggaa accgaggtgt
    69361 aggcgatcag acgtttgatg tcggtctggg cgaaggccat ccacgcaccg tagaagatgc
    69421 cgataacacc cagccacata gcgattggcg caaactctgc cgacgcgttc gggaacagcg
    69481 gcagggagaa acgcagcaaa ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa
    69541 cggaaccggc ggtcggagcc tgggagtgcg catccggcag ccagccatgc agcggaacca
    69601 ccggcatttt gactgcgaag gcgatgaaga agcccagcat taacaggtat tccacaccac
    69661 tggacattgg cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt
    69721 agtgaacaaa aaccagcgcc aggatggcga tcaacatcac cagaccactc gcctgggtgt
    69781 aaatgaagaa cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc
    69841 acagtgcgat caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga
    69901 acatgtcgat ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt
    69961 ggaagaagcc ctgatatttt tcgatctctt tccacgaaca gagtaccgcc agcacaccga
    70021 gcagaccggt cagcacgacc atcagcagcg acagcccgtc aatggcgaga tgaatagaga
    70081 taccaaaacg cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg
    70141 attgcgtcag tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc
    70201 ccatggtgat cagcgcgatc cagcgcggca ccttgacgcc aaagcgttcg gtctgccagc
    70261 acaggaagcc gccgataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta
    70321 ttccttgtaa aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct
    70381 cttcggactg ggggtagtgt cggatgcacc gcgaatgcgg cttatccgac ctacgttctt
    70441 atatgccaag gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc
    70501 accacgaccg caccgatgct catggatgcc acataccagc gcagatagcc gttctcactt
    70561 aacagcagac ctttacctgc aaagcgggaa aggacagccg ggatgttcat cattgagttc
    70621 agcggatcgc gtttcagcaa ccaggcaata cccaggaatg gcttgacgaa cactttgtcg
    70681 tacagccagt caaagcccca ggcgttgtac caccaggtgc ccagcagacg gcccggcgca
    70741 ctgttggcga tggaggtcac cagagtacgt ttacccagcc acagccaggc tgccagcaga
    70801 atgccgacca ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc
    70861 agttccgtcg tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag
    70921 gtggaaagga tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga
    70981 gcgtgaattt gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag
    71041 gtcataaacg cacccaccag acctgccacc atcagattga tatgaccatt cgccatcgca
    71101 cccgcgagga tctcatcctt actgaagaag cccgcagtga ccagcggcag tgccgacagt
    71161 gctgcgccgc ccaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc
    71221 atcttgaaga tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac
    71281 agcagcgctt taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc
    71341 acgccaagcg cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt
    71401 ttgatgtcgg tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccaaca
    71461 atgcccacca gatgcagaac ttccggggtc atcaggaaca ggccgtgggt acgggcgatc
    71521 aggtagacac ccgcggttac catcgttgcg gcgtggatca gcgcggagac aggcgtcggg
    71581 cccgccatcg cgtcagcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca
    71641 ccgcccagca gcatcagcgt cgcccacatc agcatgttat tgccatcagc aaagtgcgct
    71701 ggtgccagtt ccaccatttc gcggaagttc agggtgccca gttcgttgta aagaatgaac
    71761 agtgcgaaag cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt cattgccgct
    71821 gcgccattct tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg
    71881 ccttcccagc cgaggtacat cagcagcagg ttgtcggcaa gcaccagaac taccatgctg
    71941 gcgatgaaca ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac
    72001 caggaggcgt acatgtggat caggaaaccc acaccagtga ccactgagag catggtcagc
    72061 gacaggccgt ccaacaccag gttaaaaccg atgttaaagt cgcctaccga catccacgtc
    72121 cacagcggct ggctgtatgc ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg
    72181 gttaccagcg ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac
    72241 cagcgcccac gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag
    72301 gcaagcatgt tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac
    72361 ggtgaagttg cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg
    72421 cgagaatgta catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg
    72481 ccagcgcgga ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac
    72541 ggataaccag accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt
    72601 gtaaggggat catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct
    72661 cagcacttca cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag
    72721 cagcagcata gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac
    72781 tgctttagca ctgattggcg taccgtcgat accctgatcg ttaacaccga ggatggcgta
    72841 aacaatcacc accagcatga tggccgacaa aattgccgga ccaatccaca cctgcggttt
    72901 cagccactgg cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa
    72961 cacgaacagc accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta
    73021 agcgcctagt gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag
    73081 tgcgtgtacc ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc
    73141 acagatataa aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg
    73201 ataggcttgg cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc
    73261 atccggtaga agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttc
    73321 tcgtaaacca gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc
    73381 gccgtggtcg gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttgata
    73441 cggaaaaatt ccggatacca gcgaccgtct ttggtttctg ccttttgcag cgagatacag
    73501 ccgaccgggc aggctaccgc acagaggtta caggctacgc aacgctcttc gccgtccggg
    73561 tcgcgggtca gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg
    73621 tacattcgcg tttcgcgttt tgcgaacgcg tgcaggccga tcatccagat actacgaacc
    73681 tgggtgccga aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg
    73741 cctgccagag aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga
    73801 ttttccagcc gaaggacatt acctggtcat aacgcggacg cggtaacgac gcacgaatca
    73861 aaatgaacat catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca
    73921 acgggccttg ccagccaccg aagaacagcg tcaccatcaa tgcagagatg gtcacaatcc
    73981 cgatgtactc acccacgaag aacagaccga acttcatacc ggaatattca atgtggtaac
    74041 catccgccag ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cacaccgcca
    74101 cgcccgcgat ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc cacacatgcg
    74161 cctggctgtt gacgatgtcg gtcatattga atgaaccggc ctgcgccacc acgcccatca
    74221 gggaaagccc gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccga
    74281 gcagcgagta tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa actgccagac
    74341 ctgccatcat caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccggac
    74401 tgactggcac aattgcaaag gccagcagca gcgaggtaaa ggcaatcatc ggtgccaggg
    74461 taaagatgac gcgatccgag aacttcggga tccagtcttc tttaaagaac attttgatca
    74521 tatccgcaac caactggagc gaaccgcccc agccaacacg gttaggtccg taacggttct
    74581 ggaacagacc cagcaggcga cgttcgccaa agctcatgaa tgccccgcag gtgacaacca
    74641 ccagcaggat caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata
    74701 tccaactcat tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc
    74761 aatgccggac atacccatcg gcaagcccac ctgccctgcc gtcagccctt cggcgatttc
    74821 aaccggcagc gtgaccgtgt tgccatcgta actaaaggag acgcgtgtac ctgcgtttac
    74881 acccaacttc gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacgactctg
    74941 gaagaccgga gcacgctgtg acaattcatc actgccaaac aggtggtagt acggcgcaat
    75001 acgccatttc ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc
    75061 attttcgctg gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac
    75121 ttcgtcctgg aatttgttcc acgcctgcgg ggagttccag cccggagccc aggcaaacgg
    75181 cacttgcgaa cggtgcgcgg tcggctggtt gttaccttcc atcgagaagg tgaacatggt
    75241 gtcaatatcc tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg
    75301 gccgctgtaa cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc
    75361 cggcgcagca tctttgatac ctgccagttc cgggattttc gccacaacag cgtcaatcac
    75421 atggtcgagc tgcgtccagt ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa
    75481 ccaacgccag ctttccagca tgacagtttt gctatcgtaa taagccggat cgtaaacctg
    75541 gaagaaacgt tgggcgcgac cttcattatt aattaccgta ccgtcgcttt cagcaaagct
    75601 ggcagcggaa agcaccagat gggcgttttc catgatcgct gtgcgttgat gatcaaccac
    75661 catcaccagc ggcgctttag ccagcgcagc attcacgcgg gtagcggaag cgtgacgatg
    75721 cagatcgttt tccagcacca ccaccgcgtc ggcgcgtccg gtttccagtt cggtcaacgc
    75781 ttcttcaagc gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat
    75841 catggtgata ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc
    75901 ctgaatcacc tctaagctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc
    75961 tgccagtgcc tgcacgatga cgtcgatttt gctttgcagc tcaggttcga taccgtcaac
    76021 cgctggtgca gagttatcca gcgcatgggc aatggcaaaa cctaaacgcg cctgatcttc
    76081 aaccggtgcg cggtaagtcc acgccgcgat atcatccaga cgagtgtcat caacgttggt
    76141 aacaaacagc ggatgcttag cacgttgacc gatgttgagg attgccgcaa tctgccagtc
    76201 agccactttc tgtgctgccg ccatttcgcg cgctttacct ttcaccgcct gacgcactgc
    76261 cagcgcgacg cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta
    76321 agattcgatt tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc
    76381 cagttgcaga cgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac
    76441 cagttcacgc agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac
    76501 tttcttcgac tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt
    76561 aatgaaatca tcgccacgac gctgtactgg ctgacgcgga cgatccttca ggttgacgta
    76621 accgtaaccg aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg
    76681 gttttcgata cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga
    76741 acattgctgg cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga
    76801 gtgcgttttg tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc
    76861 gctttccagc gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg caccgtaaac
    76921 gcccagatct gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt agcaggcgat
    76981 gcagcggttc atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt
    77041 ggtgaaacgg tagcgacgga agctgtgtcc ggtcatcacg gtcatatcct gaagatggca
    77101 gttaccgcct tcttcacata ccggacagtc gtgcgggtgg ttggtcatta accactcgac
    77161 cacactttca cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga
    77221 agccggtgtc atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg
    77281 cttcaccgca cactggcggc aagcaccgac gcttcccagc gccggatgcc agcaaaagta
    77341 aggaatatca aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc
    77401 gtattctttg ccgtctacat gaattgtagc cattagcatg cttccagttt tctcggtcag
    77461 ggaccgagcg ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc
    77521 cattaatcaa atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga
    77581 aatatttgat ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt
    77641 tacccgggcc taagaatcga cacagttgct caagtgtttc gatatctccc ggctgacctt
    77701 cgccacgctc cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg
    77761 tacaccagcc gcaggactca cgggcgaaaa actcttccag attacgcacc agcgacacca
    77821 tgttgatctc atggtcaacc gccatcgcca gcgccgtacc cagacggctt cccgctttac
    77881 cgatactttc gaattccatc ggcaggtcaa ggtgcgcttc agtcaggaaa tcagtccccg
    77941 cgccgcctgg ctgccaggct ttgaatttca gaccgtcacg cataccaccg gcgtaatctt
    78001 caaggatttc acgcgcggta gtaccgaacg gcagttccca caggcccgga tttttcaccc
    78061 gaccagagaa gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc
    78121 actccacgcc gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacgc
    78181 aggttggttt accccatgca ccggaggttg ccgggaaagg tggcttcgag cgtgggttag
    78241 cgcgacgccc ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg
    78301 ccccggtatg gacgaacagt tcgaagtcaa aacctgttcc cataatattt ttgccaagca
    78361 aacctgcttc ggtggcttcg gcaatggcac ggcgcagatt cactgccgct tcgatatatt
    78421 cgccacgcag gaagatgtag ccacggtaag ctttcagcgc aaacgcggag ataagcatac
    78481 cttccaccag caggtgcggc agttgctcca tcagaaggcg gtctttatag gtgcccggct
    78541 ccatttcatc ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca
    78601 ggctccattt caggccggta gaaaagcccg cgccgccgcg ccctttcaga ccagcgtctt
    78661 ttacctgatt aacgatttcg tccggagaca gcccggtcag cgccttacgc gccccttcgt
    78721 aaccgttttt gctgcgatat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc
    78781 aggtcagcgg atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag
    78841 ttcagggatc gcttccgggg tcagatgcgc gtgagtgtcc tcatcgatca tcatgtttgg
    78901 ccctttatca cagttcccca ggcagcaagt tggcagcagc gtaaagcgac cgtcaaatgt
    78961 cgtttgccct ggtttgatgt ttagcttttt ctcgagcgcc gcctgaatac cctggtaacc
    79021 gttgatatga cagaccacgc tgtcgcaata acggatcaca tggcggccaa ccggctggcg
    79081 gaagatctgg ctgtagaacg ttgccacgcc ttcgacgtcg cttgccggaa tacccagcac
    79141 atcggcgatc gcgtggatcg cgccatccgg cacccagcca cgctgcttct gaacgatttt
    79201 cagcgcttca atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc
    79261 ttcacgctct gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat
    79321 aattagcggt ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca
    79381 gacaccaggc tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg
    79441 cggatacgag tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc
    79501 cccttcgtcg cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac
    79561 acttgcaaga agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc
    79621 ggcgtggtca gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc
    79681 tcaagaatgc gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag
    79741 tcagaaacac caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga
    79801 cgcgccttac gcacgtcgaa gtcgatcccg gtggcacgca ggcccgcgcc ggtggtgccc
    79861 cactccagcg cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg
    79921 gtattttgca gcgctgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac
    79981 tcacgcagca ggcgatccca gccacgcggc aggtcgtgcg ctacgccgcc aatacggaac
    80041 cacgccgggt gcatacggaa accagtgatt gcttccacca gatcgtaaat tttctgacga
    80101 tcggtaaagg cgaagaacac tggcgtcatt gcgccgacgt cctgaataaa ggtcgagata
    80161 tacagcaggt ggctgttgat gcggaacagc tcagaaagca ttacgcgaat gacgttaacg
    80221 cgatccggca cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta
    80281 acgcagccgc cgaggtattc gatacggtcg gtatacggaa tgtagctgtg ccaggactgg
    80341 cgttcgccca ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca
    80401 atctcttcgc catcgagctg caaaacgata cggaaagcac cgtgcgccga cgggtggttc
    80461 ggaccgaggt tgaggaacat gaagtcctcg ttttcagtac cacgcttcat cccccactct
    80521 tccggtttga aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac
    80581 ggcgagaatt cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc tttccaggtt
    80641 tgcggcatca tgatgcggcg caggttcggg tgaccgtcga atgtaatgcc aaacagatcc
    80701 caggtttcac gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc
    80761 aggtcgtttt ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc
    80821 agatggtaga aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt
    80881 tcgtccatgc cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa
    80941 tcgccaactt ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc
    81001 gcctgaacag taaaggcatc cggcccaaaa cggttgcgca gttcgccaat taccggatca
    81061 tcaagatgat cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc
    81121 accattgcaa atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg
    81181 aataagcgaa gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc
    81241 aggttggtta cggcaatgcg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg
    81301 cgataaacgc cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat
    81361 tcctgcaaca gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata
    81421 tacacatcaa ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata
    81481 ccaccagagt tggcacaggc acccattgag ataacccatt ttggttccag catctggtca
    81541 tacagacgct gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg
    81601 tcagcctgac gcggggaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc
    81661 gcggtaaacg aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa
    81721 attgagtttt tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg
    81781 ttaacttctt gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg
    81841 ttctcaccgt tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag
    81901 cgttgacgat tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg
    81961 ggcgtccagt ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa
    82021 ataaaaattg cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat
    82081 gcgaacagat acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac
    82141 ttggcggaca ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg
    82201 tttttcgacc tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa
    82261 cacaggccaa tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca
    82321 gtggatgttg acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc
    82381 acggcagatg gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaatcactg
    82441 tagaaa
//
LOCUS       DAZFXT010000006        82254 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.006, whole genome shotgun sequence.
ACCESSION   DAZFXT010000006 DAZFXT010000000
VERSION     DAZFXT010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 82254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 82254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.006"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(108..3632)
                     /gene="nifJ"
                     /locus_tag="ACHKW0_000422"
     CDS             complement(108..3632)
                     /gene="nifJ"
                     /locus_tag="ACHKW0_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HFO7473312.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKCQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSVALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
     gene            3906..4172
                     /gene="ydbJ"
                     /locus_tag="ACHKW0_000423"
     CDS             3906..4172
                     /gene="ydbJ"
                     /locus_tag="ACHKW0_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HFO7473313.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            complement(4169..4591)
                     /gene="hslJ"
                     /locus_tag="ACHKW0_000424"
     CDS             complement(4169..4591)
                     /gene="hslJ"
                     /locus_tag="ACHKW0_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HFO7473314.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(4702..5691)
                     /gene="ldhA"
                     /locus_tag="ACHKW0_000425"
     CDS             complement(4702..5691)
                     /gene="ldhA"
                     /locus_tag="ACHKW0_000425"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7473315.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            5899..8538
                     /gene="ydbH"
                     /locus_tag="ACHKW0_000426"
     CDS             5899..8538
                     /gene="ydbH"
                     /locus_tag="ACHKW0_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HFO7473316.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            8535..8720
                     /gene="ynbE"
                     /locus_tag="ACHKW0_000427"
     CDS             8535..8720
                     /gene="ynbE"
                     /locus_tag="ACHKW0_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HFO7473317.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            8725..9054
                     /gene="ydbL"
                     /locus_tag="ACHKW0_000428"
     CDS             8725..9054
                     /gene="ydbL"
                     /locus_tag="ACHKW0_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HFO7473318.1"
                     /translation="MMKKTLLLCAFLVGLVSSNIMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(9226..10131)
                     /gene="feaR"
                     /locus_tag="ACHKW0_000429"
     CDS             complement(9226..10131)
                     /gene="feaR"
                     /locus_tag="ACHKW0_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HFO7473319.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLETHFAKGL
                     KLSTVTTNGVNLYRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            10367..11866
                     /gene="feaB"
                     /locus_tag="ACHKW0_000430"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             10367..11866
                     /gene="feaB"
                     /locus_tag="ACHKW0_000430"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HFO7473320.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADHLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGSNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDGWCETKSVCVRY"
     gene            complement(11924..14197)
                     /gene="tynA"
                     /locus_tag="ACHKW0_000431"
     CDS             complement(11924..14197)
                     /gene="tynA"
                     /locus_tag="ACHKW0_000431"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0008131 - primary amine oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0009308 - amine metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HFO7473321.1"
                     /translation="MGSPSLYSARKTTLALAVALSFAWQAPVFAHGGEAHMVPMDKTL
                     KEFGADVQWDDYAQLFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPTKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDSRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(14445..16490)
                     /gene="paaZ"
                     /locus_tag="ACHKW0_000432"
     CDS             complement(14445..16490)
                     /gene="paaZ"
                     /locus_tag="ACHKW0_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HFO7473322.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RLFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELLPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSATTGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDITPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQTLVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNTLLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            16775..17704
                     /gene="paaA"
                     /locus_tag="ACHKW0_000433"
     CDS             16775..17704
                     /gene="paaA"
                     /locus_tag="ACHKW0_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HFO7473323.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWKEGTWVREAALAHAQKQHARKVA"
     gene            17716..18003
                     /gene="paaB"
                     /locus_tag="ACHKW0_000434"
     CDS             17716..18003
                     /gene="paaB"
                     /locus_tag="ACHKW0_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HFO7473324.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            18012..18758
                     /gene="paaC"
                     /locus_tag="ACHKW0_000435"
     CDS             18012..18758
                     /gene="paaC"
                     /locus_tag="ACHKW0_000435"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HFO7473325.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAIN
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            18773..19270
                     /gene="paaD"
                     /locus_tag="ACHKW0_000436"
     CDS             18773..19270
                     /gene="paaD"
                     /locus_tag="ACHKW0_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaD"
                     /protein_id="HFO7473326.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            19278..20348
                     /gene="paaE"
                     /locus_tag="ACHKW0_000437"
     CDS             19278..20348
                     /gene="paaE"
                     /locus_tag="ACHKW0_000437"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HFO7473327.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            20345..21112
                     /gene="paaF"
                     /locus_tag="ACHKW0_000438"
     CDS             20345..21112
                     /gene="paaF"
                     /locus_tag="ACHKW0_000438"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase PaaF"
                     /protein_id="HFO7473328.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            21112..21900
                     /gene="paaG"
                     /locus_tag="ACHKW0_000439"
     CDS             21112..21900
                     /gene="paaG"
                     /locus_tag="ACHKW0_000439"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HFO7473329.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLECDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            21902..23329
                     /gene="paaH"
                     /locus_tag="ACHKW0_000440"
     CDS             21902..23329
                     /gene="paaH"
                     /locus_tag="ACHKW0_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008691 - 3-hydroxybutyryl-CoA
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaH"
                     /protein_id="HFO7473330.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            23319..23741
                     /gene="paaI"
                     /locus_tag="ACHKW0_000441"
     CDS             23319..23741
                     /gene="paaI"
                     /locus_tag="ACHKW0_000441"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HFO7473331.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            23741..24946
                     /gene="paaJ"
                     /locus_tag="ACHKW0_000442"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             23741..24946
                     /gene="paaJ"
                     /locus_tag="ACHKW0_000442"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HFO7473332.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            24973..26286
                     /gene="paaK"
                     /locus_tag="ACHKW0_000443"
     CDS             24973..26286
                     /gene="paaK"
                     /locus_tag="ACHKW0_000443"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047475 - phenylacetate-CoA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase PaaK"
                     /protein_id="HFO7473333.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGSPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            26387..27337
                     /gene="paaX"
                     /locus_tag="ACHKW0_000444"
     CDS             26387..27337
                     /gene="paaX"
                     /locus_tag="ACHKW0_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HFO7473334.1"
                     /translation="MSKLDTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKATLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVQLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPSPGSLYFQRFGGLNIEQEALCQFIR"
     gene            27319..27909
                     /gene="paaY"
                     /locus_tag="ACHKW0_000445"
     CDS             27319..27909
                     /gene="paaY"
                     /locus_tag="ACHKW0_000445"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HFO7473335.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHMGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKLIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPGRKRLVFDENLRPKQ"
     gene            28141..29001
                     /gene="pdxI"
                     /locus_tag="ACHKW0_000446"
     CDS             28141..29001
                     /gene="pdxI"
                     /locus_tag="ACHKW0_000446"
                     /EC_number="1.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HFO7473336.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            29065..31371
                     /gene="ydbD"
                     /locus_tag="ACHKW0_000447"
     CDS             29065..31371
                     /gene="ydbD"
                     /locus_tag="ACHKW0_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HFO7473337.1"
                     /translation="MFKARICGWIGLLPLFMLSLPVQAELRCVANTVDIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSSIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPAVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            31542..32147
                     /gene="ynbA"
                     /locus_tag="ACHKW0_000448"
     CDS             31542..32147
                     /gene="ynbA"
                     /locus_tag="ACHKW0_000448"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7473338.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMSAER"
     gene            32147..33043
                     /gene="ynbB"
                     /locus_tag="ACHKW0_000449"
     CDS             32147..33043
                     /gene="ynbB"
                     /locus_tag="ACHKW0_000449"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7473339.1"
                     /translation="MLEKSLATLFALLILATLINHFLVWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFVGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYSCY"
     gene            33059..34816
                     /gene="ynbC"
                     /locus_tag="ACHKW0_000450"
     CDS             33059..34816
                     /gene="ynbC"
                     /locus_tag="ACHKW0_000450"
                     /EC_number="2.1.1.-"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HFO7473340.1"
                     /translation="MENSRIPGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            34806..36122
                     /gene="ynbD"
                     /locus_tag="ACHKW0_000451"
     CDS             34806..36122
                     /gene="ynbD"
                     /locus_tag="ACHKW0_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HFO7473341.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMMMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPCRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVDEAISYIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(36173..36778)
                     /gene="azoR"
                     /locus_tag="ACHKW0_000452"
     CDS             complement(36173..36778)
                     /gene="azoR"
                     /locus_tag="ACHKW0_000452"
                     /EC_number="1.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HFO7473342.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            36979..40881
                     /gene="hrpA"
                     /locus_tag="ACHKW0_000453"
     CDS             36979..40881
                     /gene="hrpA"
                     /locus_tag="ACHKW0_000453"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HFO7473343.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            41153..41953
                     /gene="ydcF"
                     /locus_tag="ACHKW0_000454"
     CDS             41153..41953
                     /gene="ydcF"
                     /locus_tag="ACHKW0_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF218 domain-containing protein YdcF"
                     /protein_id="HFO7473344.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            42150..43589
                     /gene="aldA"
                     /locus_tag="ACHKW0_000455"
     CDS             42150..43589
                     /gene="aldA"
                     /locus_tag="ACHKW0_000455"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO7473345.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(43631..44632)
                     /gene="gap"
                     /locus_tag="ACHKW0_000456"
     CDS             complement(43631..44632)
                     /gene="gap"
                     /locus_tag="ACHKW0_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7473346.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            44821..45351
                     /gene="cybB"
                     /locus_tag="ACHKW0_000457"
     CDS             44821..45351
                     /gene="cybB"
                     /locus_tag="ACHKW0_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HFO7473347.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            45596..45769
                     /locus_tag="ACHKW0_000458"
     CDS             45596..45769
                     /locus_tag="ACHKW0_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473348.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            45795..45863
                     /gene="yncP"
                     /locus_tag="ACHKW0_000459"
     CDS             45795..45863
                     /gene="yncP"
                     /locus_tag="ACHKW0_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YncP"
                     /protein_id="HFO7473349.1"
                     /translation="MNLLVRCAGKIPALALTWTCRP"
     gene            complement(45841..45990)
                     /gene="hokB"
                     /locus_tag="ACHKW0_000460"
     CDS             complement(45841..45990)
                     /gene="hokB"
                     /locus_tag="ACHKW0_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HFO7473350.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            46389..48029
                     /gene="trg"
                     /locus_tag="ACHKW0_000461"
     CDS             46389..48029
                     /gene="trg"
                     /locus_tag="ACHKW0_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HFO7473351.1"
                     /translation="MNTTPSHRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(48067..48990)
                     /gene="ydcI"
                     /locus_tag="ACHKW0_000462"
     CDS             complement(48067..48990)
                     /gene="ydcI"
                     /locus_tag="ACHKW0_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7473352.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            49207..50550
                     /gene="ydcJ"
                     /locus_tag="ACHKW0_000463"
     CDS             49207..50550
                     /gene="ydcJ"
                     /locus_tag="ACHKW0_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7473353.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            50775..52430
                     /gene="opgD"
                     /locus_tag="ACHKW0_000464"
                     /gene_synonym="mdoD"
     CDS             50775..52430
                     /gene="opgD"
                     /locus_tag="ACHKW0_000464"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HFO7473354.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            52570..52794
                     /gene="ydcH"
                     /locus_tag="ACHKW0_000465"
     CDS             52570..52794
                     /gene="ydcH"
                     /locus_tag="ACHKW0_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HFO7473355.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            52857..53393
                     /gene="rimL"
                     /locus_tag="ACHKW0_000466"
     CDS             52857..53393
                     /gene="rimL"
                     /locus_tag="ACHKW0_000466"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HFO7473356.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(53388..54368)
                     /gene="ydcK"
                     /locus_tag="ACHKW0_000467"
     CDS             complement(53388..54368)
                     /gene="ydcK"
                     /locus_tag="ACHKW0_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcK family protein"
                     /protein_id="HFO7473357.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            54492..55484
                     /gene="tehA"
                     /locus_tag="ACHKW0_000468"
     CDS             54492..55484
                     /gene="tehA"
                     /locus_tag="ACHKW0_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HFO7473358.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            55481..56074
                     /gene="tehB"
                     /locus_tag="ACHKW0_000469"
     CDS             55481..56074
                     /gene="tehB"
                     /locus_tag="ACHKW0_000469"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0046690 - response to tellurium ion
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HFO7473359.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIVNMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            56377..57045
                     /gene="ydcL"
                     /locus_tag="ACHKW0_000470"
     CDS             56377..57045
                     /gene="ydcL"
                     /locus_tag="ACHKW0_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HFO7473360.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPLVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(57144..57506)
                     /gene="tnpA"
                     /locus_tag="ACHKW0_000471"
                     /pseudo
     CDS             complement(57144..57506)
                     /gene="tnpA"
                     /locus_tag="ACHKW0_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278583.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            57576..58784
                     /gene="insQ"
                     /locus_tag="ACHKW0_000472"
     CDS             57576..58784
                     /gene="insQ"
                     /locus_tag="ACHKW0_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO7473361.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTPARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(58824..60038)
                     /gene="ydcO"
                     /locus_tag="ACHKW0_000473"
     CDS             complement(58824..60038)
                     /gene="ydcO"
                     /locus_tag="ACHKW0_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HFO7473362.1"
                     /translation="MLRCLYAITHEVTMRLFSVPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIFTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSTPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAGAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            60091..60627
                     /gene="sutR"
                     /locus_tag="ACHKW0_000474"
     CDS             60091..60627
                     /gene="sutR"
                     /locus_tag="ACHKW0_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HFO7473363.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQPATPSVYDPQQHAMVITSLFPYDPQLCFEHF
                     SIQMAPGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            60700..62661
                     /gene="rlhA"
                     /locus_tag="ACHKW0_000475"
     CDS             60700..62661
                     /gene="rlhA"
                     /locus_tag="ACHKW0_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HFO7473364.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTVNLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTRENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(62753..62983)
                     /gene="yncJ"
                     /locus_tag="ACHKW0_000476"
     CDS             complement(62753..62983)
                     /gene="yncJ"
                     /locus_tag="ACHKW0_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HFO7473365.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            63205..63359
                     /locus_tag="ACHKW0_000477"
                     /pseudo
     CDS             63205..63359
                     /locus_tag="ACHKW0_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            63405..63821
                     /gene="hicB"
                     /locus_tag="ACHKW0_000478"
     CDS             63405..63821
                     /gene="hicB"
                     /locus_tag="ACHKW0_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HFO7473366.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            63900..65306
                     /gene="ydcR"
                     /locus_tag="ACHKW0_000479"
     CDS             63900..65306
                     /gene="ydcR"
                     /locus_tag="ACHKW0_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7473367.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHNDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            65551..66696
                     /gene="ydcS"
                     /locus_tag="ACHKW0_000480"
     CDS             65551..66696
                     /gene="ydcS"
                     /locus_tag="ACHKW0_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter substrate-binding
                     protein YdcS"
                     /protein_id="HFO7473368.1"
                     /translation="MSKTFARSSLCALSMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            66714..67727
                     /gene="ydcT"
                     /locus_tag="ACHKW0_000481"
     CDS             66714..67727
                     /gene="ydcT"
                     /locus_tag="ACHKW0_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7473369.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            67728..68669
                     /gene="ydcU"
                     /locus_tag="ACHKW0_000482"
     CDS             67728..68669
                     /gene="ydcU"
                     /locus_tag="ACHKW0_000482"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7473370.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPGLGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            68659..69453
                     /gene="ydcV"
                     /locus_tag="ACHKW0_000483"
     CDS             68659..69453
                     /gene="ydcV"
                     /locus_tag="ACHKW0_000483"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7473371.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLATAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            69475..70899
                     /gene="patD"
                     /locus_tag="ACHKW0_000484"
     CDS             69475..70899
                     /gene="patD"
                     /locus_tag="ACHKW0_000484"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019145 - aminobutyraldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HFO7473372.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            71109..71918
                     /locus_tag="ACHKW0_000485"
     CDS             71109..71918
                     /locus_tag="ACHKW0_000485"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HFO7473373.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISDGNIQERDYKKFNLSEVFRGL"
     gene            complement(72320..72415)
                     /gene="yncL"
                     /locus_tag="ACHKW0_000486"
     CDS             complement(72320..72415)
                     /gene="yncL"
                     /locus_tag="ACHKW0_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HFO7473374.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            72610..72783
                     /gene="ortT"
                     /locus_tag="ACHKW0_000487"
     CDS             72610..72783
                     /gene="ortT"
                     /locus_tag="ACHKW0_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HFO7473375.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            72869..73102
                     /gene="ydcY"
                     /locus_tag="ACHKW0_000488"
     CDS             72869..73102
                     /gene="ydcY"
                     /locus_tag="ACHKW0_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2526 family protein YdcY"
                     /protein_id="HFO7473376.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(73103..73552)
                     /gene="ydcZ"
                     /locus_tag="ACHKW0_000489"
     CDS             complement(73103..73552)
                     /gene="ydcZ"
                     /locus_tag="ACHKW0_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7473377.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(73549..74067)
                     /gene="mddA"
                     /locus_tag="ACHKW0_000490"
     CDS             complement(73549..74067)
                     /gene="mddA"
                     /locus_tag="ACHKW0_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HFO7473378.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            74248..75285
                     /gene="curA"
                     /locus_tag="ACHKW0_000491"
     CDS             74248..75285
                     /gene="curA"
                     /locus_tag="ACHKW0_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HFO7473379.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            75483..76148
                     /gene="mcbR"
                     /locus_tag="ACHKW0_000492"
     CDS             75483..76148
                     /gene="mcbR"
                     /locus_tag="ACHKW0_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HFO7473380.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(76184..78286)
                     /gene="pqqU"
                     /locus_tag="ACHKW0_000493"
     CDS             complement(76184..78286)
                     /gene="pqqU"
                     /locus_tag="ACHKW0_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor PqqU"
                     /protein_id="HFO7473381.1"
                     /translation="MKIFSVRQTVLPALLVLSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATSRINLSESLTGVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            78528..79589
                     /gene="yncE"
                     /locus_tag="ACHKW0_000494"
     CDS             78528..79589
                     /gene="yncE"
                     /locus_tag="ACHKW0_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HFO7473382.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKDSVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            complement(79702..81201)
                     /gene="ansP"
                     /locus_tag="ACHKW0_000495"
     CDS             complement(79702..81201)
                     /gene="ansP"
                     /locus_tag="ACHKW0_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HFO7473383.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            81468..82085
                     /gene="yncG"
                     /locus_tag="ACHKW0_000496"
     CDS             81468..82085
                     /gene="yncG"
                     /locus_tag="ACHKW0_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HFO7473384.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            82161..>82254
                     /locus_tag="ACHKW0_000497"
     CDS             82161..>82254
                     /locus_tag="ACHKW0_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473385.1"
                     /translation="MLCFLIYITLPFIQLVYFISSEKKLTIHIVQ"
BASE COUNT        20575 a        19627 c        21430 g        20622 t
ORIGIN      
        1 ttctgttggg ccattgcatt gccactgatt ttccaacata taaaaagaca agcccgaaca
       61 gtcgtccggg ctttttttta gaattggata atccttatcc agagcattta atcggtgttg
      121 cttttttccg cttttccggc catttgtgcc aggaagtcat agcgtttttg caaatcagct
      181 gcagcatctt tccataactg ttctgccact tctggctgct gcgaattcag ccgacggaaa
      241 cgttgctcgt gaagtaacgt ttcttccagt gctactgacg gcgggcgtga atccagcgcc
      301 agcggcagtt tgccttcatc ggcacgacgc ggatcgaagc gatatagcgg ccagaagccg
      361 gtagctgtga gttggcgcat ctggtcgtgg ctgagtgcca gatcgtaacc atgctcttca
      421 catgggctat aagcgatgat cagcgatggc cccggatacg cttccgcttc ctgaatcgct
      481 ttcaccgtct ggttgagctg cgcgccgaga gaaatctgcg ccacataaac atgaccgtac
      541 atcatcatac tgacgccaag atctttacgc gctttacgtt tgccgtgctc gccaaattta
      601 gttactgcac ccagcggtgt cgctttcgac gcctgaccac cggtgttgga atagcattgc
      661 gtatccagca ccagaatgtt gacgttttcc gtcaaactca atacatgatc cagaccgcca
      721 aagccgatat cgtaagccca gccatcacca ccaatcagcc agattgattt ttccaccagt
      781 gcatctgcat cacgtagcag ttcatgtgct tcggcaacat cgttgagttg ctggcgtaaa
      841 gctgcgacct gttctcgacg aacctctggc gtagcgtctg atttcaacgc cgtcagtaat
      901 tccgccggga ttttatcggc aaattgatcc agcagacgca gcacgcggac acggtgttga
      961 tcgaccgtca ggcggaaacc aaggccaaat tcggcattat cttcaaatag agagttcgcc
     1021 catgccggcc cacgaccgtt ggcatcggtg gtatacggtg tagagggcag gttaccgcca
     1081 taaattgaag aacagccggt ggcgttagcg atcaacatcc ggtcgccata gagctgagtc
     1141 agtaatttaa tatacggcgt ctcgccacaa ccggagcaag cacctgaata ttcaaacagc
     1201 ggtgtaatca gctgcgatgt acgaatatca atacgttcca gtttgctacg gtcgatttct
     1261 ggcaggttga ggaagaaatc gtaattgatt ttctcttctt cgacatgttc caggcgcgac
     1321 atcatattga tggctttaat ctctggattc tgacggtctt tcgccgggca aacttcgacg
     1381 cacaggttac aaccggtgca atcttccggt gccacctgca agacatattt ctgcccgcgc
     1441 atatcacgcg atttcacatc cagcgaatgc aggctggcag gggcgttttc catcgcttca
     1501 ggcggcacca cttttgcgcg aatagctgag tgtgggcaag cggcaacgca gtggttacat
     1561 tgggtacaga gttcctcttt ccagatgggg atctcttcgg cgatattgcg tttttcccag
     1621 cgagtggtgc ccatcggcca ggtgccgtct ggcggcagcg ccgaaacggg gagggcatca
     1681 ccaagccctg cgagcatcgc tgcggttacg gttttcacga aatcaggggc ggcatcggaa
     1741 accactggcg gtcgattggc gctgtgcgga tttaccggtt gcaacggaac ttcttctacg
     1801 gattcacgcg ccagcgccag agcctgccag ttgcgttcca ccagatcctg gcctttgcta
     1861 ctgtaacttt tggcaatcgc accctgcaat tctgcgaggg cgctatcgcc aggcagaatt
     1921 tgcgtcagat ggaaaaaagc catctgcatg acggtattaa tacgggccgc caggccacat
     1981 tcgcgggcga ttttcgccgc gttaatcaca tagaagcgcg cttttttctg atttagcacg
     2041 gcctgaactt cctgcggcaa gcgcgaccac acttcatctg cgctgtacgg cgtgttgagc
     2101 aggaaaatgc cgccaggttt taaacgctca gccatctgac atttatcgat aaactgcaac
     2161 tggtggcagc caacaaaatc agcctgggaa atgagataag cggaacgaat cggctgttcg
     2221 ctcacgcgaa ggtgagaaac cgtcaggccg cccgcctttt tggagtcgta aacaaaatag
     2281 ccctgtgcgt accacggcgt ggaattaccg ataatcttga tattgttttt ggtcgcggaa
     2341 acgctgccat cactaccaag gccataaaac aaggcttcca gtttcgccga gtttggcagg
     2401 gtgttttccg gcaacggcag tgacagattg gtcacatcat cgtaaatacc aaccgtaaag
     2461 cgcgctttcg gtttagccgc gttgagctcg gcaaataccg ccagtacaca gtccgggcca
     2521 aattctttgg atgaaagacc atagcgccca ccaatgacac ggggcagagt ttcgcgctcg
     2581 ccattattaa aggcttctgc cagtgcggtc attacatcca gatagagcgg ttctgcctgg
     2641 gcaccgggtt ctttggttct gtccagtacc gccacgctgc gtacggatcc cggcagagct
     2701 tgcagcaagt gtttggcgga gaagggacgg tacaggcgaa ctttcagcac gccaactttt
     2761 tcgccacggg ttagcaattc atcaaccact tcttcacagg tgccaatggc agagcccatc
     2821 aggataatca cccgttccgc ttgcggatgc ccgtaatatt caaacggctg atactgacga
     2881 cctgtcgcgg cagagaaatc attcatcgcc tgttcaacat ggtcatagac cgcgtcgtac
     2941 catgggttgg tggcttcgcg agactggaaa taagtgtcag gattggcgga cgtaccgcgg
     3001 atcaccggat gttccgggtt gagcgcccgg gcgcgatgag catcaatttc ggcctgcggc
     3061 atgagatcaa gaatagtgtc atcggccagc gggacaattt tattgatttc gtgggacgtg
     3121 cggaaaccat caaagaaatg aataaatggc acgcggctct tcagcgttgc gatgtgcgaa
     3181 atgagagcaa aatcttgcgc ttcctggacg ttagccgcac acaacatcgc gcaacccgtc
     3241 tggcgcaccg ccatgacgtc ggaatgatcg ccaaaaatag agagtgcatg tgtggcaacg
     3301 gtacgtgccg ctacatgcag gacaaacggt gtgagttcgc ctgccagttt gtacagcgtc
     3361 gggatcatca gcagcaaacc ctgcgatgac gtaaacgatg ttgaaagggc acccgtctgc
     3421 aaagcgccat gcacggtagc gatagcaccc gcttccgact gcatttcaac cacgcgtggt
     3481 gtgtctcccc aaacgttctt caagccgttt ccggcccagg catcagcctg ttctgccatc
     3541 gtggaactgg gggtaatagg gtagatggcg ataacttcac tggtgcgaaa cgccaccgaa
     3601 gcaaccgcgc cattaccgtc aatagtaatc atatgacacc cttacattgc gcaaatgagg
     3661 ggcgcacgaa attgctgcgc gcccagtagt aatccttcaa ttttagcaaa tggctttctt
     3721 ctgcattttc gcttttgtgt cccccacatc agcgtaatga atgttttgat caaacagagg
     3781 gcaaaaaaat agccataaaa aagtaaaaat gcggggcaac gcgcaggaca gctctcgacg
     3841 ccgccctctg tgctgcctat tatttatggc ggtgtcgttt ggcttgaaga tcagaggaag
     3901 aagagatgcg agcagcgttt tgggtagggt gtgccgcttt attgttgtcg gcgtgtagta
     3961 gtgaacctgt tcagcaggcg actgcggcgc acgtagcgcc aggtttaaaa gcgtcgatgt
     4021 ccagtagtgg agaagcaaat tgtgcaatga tcggcggttc gctttctgtt gcccgtcaac
     4081 tggatggtac ggcgattggg atgtgtgcat tacccaacgg caaacgctgt agcgaacagt
     4141 cacttgccgc cgggagctgt ggcagctatt aattcattaa atccgccagc ttataagtta
     4201 atgtctgttt cgcggtcgcc agcgttaact ggttcgcggt cagatccact tgtgcacctt
     4261 ctttcagcat ttcgctaatg gtgttatcga gttcattaag ctgcgggtta gcgcacatca
     4321 tacgggtcat tgccagccct ttggctgtca gttcaccatt agacagtttg ccttcaccgc
     4381 taaagcggtt acacatgctg ccggaaatca tcattttttc accaaagctg atttctggcg
     4441 gatttttatc gctggtcacg ggcttaccgt ttacgctttc cagcacaaag cgatgatgct
     4501 gtagctgttc tggcgtaaca gcaattttgt cattacttac acatcccgcc atcagcaggc
     4561 ttagcgcaac aaacgcggct actttcttca ttgtggttct caattacagt ttctgactca
     4621 ggactatttt aagaatagag aatgaaaggt cattggggat tatctgaatc agctcccctg
     4681 gattgcaggg gagcggcaag attaaaccag ttcgttcggg caggtttcac ctttttccag
     4741 attgcttaag ttttgcagcg tagtctgaga aatactggtc agagcttctg ctgtcaggaa
     4801 tgcctggtgc ccggtaaaca gcacgttgtg gcaggcagac aggcgacgga atacgtcatc
     4861 ctggatcacg tcgttggatt tatcttcaaa gaacagatcg cgttcgttct catacacgtc
     4921 catacccaac gaaccaattt tctgattttt cagcgcttca attgctgcct gagaatcaat
     4981 caatgcaccg cgactggtat tgacgatcat cacgccattt ttcatctgat cgaaggcggc
     5041 ttcgttcaac agatggtagt tttccggtgt cagcgggcag tgcagagaga taacgtctga
     5101 ttcagagaac agggttggca gatcgacata ctccacaccg agttccagcg ccgctgcact
     5161 tggatacgga tcgaacgcca gcagacgcat accaaaacct ttcagaatgc gcagcatcgc
     5221 cacaccgatt ttaccggtac cgataacgcc tgccgttttg ccatacatag taaagccggt
     5281 cagaccttcc agagagaagt tagcgtcacg ggtacgctga tacgcgcggt gaatacggcg
     5341 gttcagcgtc atcatcatac cgatggcgtg ttcagcaacg gcctctggat cataggctgg
     5401 aacacggact actttcagcc ccagttcttt tgccgcgtca aggtcgacgt tattgaaacc
     5461 ggcacagcgc agggcgatat atttaacgcc gtgctttttc agctcttcca gcaccgggcg
     5521 gctgccgtca tcgtttacga aaatacatac cgcttcgcag ccattggcag ttttagcggt
     5581 tttttccgtc agcagaaagt caaaaaattc cagctcaaag ccaaaggact cgttcacctg
     5641 ttgcaggtac ttcttgtcgt actgttttgt gctataaacg gcgagtttca taagactttc
     5701 tccagtgatg ttgaatcaca tttaagctac taaaaatatt ttacaaaatt tcaaatttaa
     5761 ttgaaagcta tggcgatatt gaaaaattca tcaacaacta tgcttagtgt aggcgcaacc
     5821 ttcaactgaa cggttaaaca tgccacaata cccgtattga atgcttaatt tttcgctaaa
     5881 tcaggatatt aactacccat gctgggtaaa tataaagccg ttctcgcgct gttattactg
     5941 attattcttg tgccgttgac gctgttgatg acgctcgggc tgtgggttcc cacgctggcg
     6001 ggcatctggc taccgctcgg gacacgtatt gcattagatg aaagcccacg cattacgcgt
     6061 aaaggtttaa tcattcccga tctccgttat ctggtgggag attgtcagct tgcgcatatc
     6121 accaacgcca gcctttcaca tcccagccgc tggttattga acgtcggcac ggtagaactt
     6181 gattctgctt gtctggcgaa attgccgcag acggagcaat cgccagctgc tccaaaaacc
     6241 ctcgcgcagt ggcagtccat gctgcctaac acctggatca atatcgataa actgattttt
     6301 tctccctggc aggaatggca gggaaaactc tctctcgcat taacctctga tatccagcaa
     6361 ctgcgttatc agggcgaaaa agttaaattt caaggccagc tgaaagggca acaacttaca
     6421 gtcagcgaac tggatgtcgt cgcgtttgaa aatcagccgc cggttaaact ggtgggggaa
     6481 tttactatgc cgctcgtgcc ggatggactt cctgtaagtg ggcatgctac tgcgacgtta
     6541 aacttgccgc aggaaccgtc actggtggat gcagagctgg actggcagga aaatagcggg
     6601 caattgattg tgctggcacg ggataacggc gatccgttgc tcgatttgcc gtggcaaatt
     6661 actcgtcaac aattgaccgt aagcgatggt cgctggagtt ggccgtatgc aggttttcct
     6721 ttgagtggcc gactgggtgt caaagtcgac aactggcagg cagggcttga gaacgctctg
     6781 atcagcggac gactgagtgt gctgacccag gggcaagcgg gtaaaggcaa cgcggtgcta
     6841 aattttggcc caggaaaatt aagcatggat aacagtcagc tgcctctgca gctgaccggt
     6901 gaagcgaaac aggcggacct cattttatat gcccgtttac ctgcgcagct aagtggaagt
     6961 ctgtctgacc caacgctgac ctttgagcca ggcgcgttac ttcgttcaaa gggaagagtc
     7021 atcgattcgc tggacatcga tgaaatccgc tggcctttag cgggtgtaaa agtcacccaa
     7081 cgtggtgttg acggacgttt gcaggccatt ttgcaggcgc atgaaaatga actgggcgat
     7141 ttcgtgctgc atatggatgg gctggcgaat gattttctcc ctgacgcggg ccgctggcag
     7201 tggcgctact ggggaaaagg cagttttaca ccgatgaatg ccacctggga tgtcgcagga
     7261 aaaggtgaat ggcatgacag cacgattacg ctgaccgacc tctccaccgg tttcgaccag
     7321 ttacaatacg gtacgatgac ggtagaaaag ccgcgattaa ttctcgacaa gcccgtcgtc
     7381 tgggtacgtg acgcacagca tccctccttc agcggcgcgc tgtcactgga cgccgggcaa
     7441 acgctgttca ctggcggcag tgtgttaccg ccatcaacct taaaatttag cgtcgatggg
     7501 cgcgatccga cgtatttcct ctttaaaggc gatttacatg ctggtgagat tggcccggtt
     7561 cgggtaaatg gtcgctggga cggtattcgt ctgcgcggta acgcctggtg gccaaaacaa
     7621 tcactgactg tattccagcc gctggtgcca cccgactgga agatgaactt acgcgatggt
     7681 gaactgtatg ctcaggttgc attttctgct gcgccggaac aaggattccg cgcgggaggg
     7741 cacggcgtgt tgaaaggcgg tagtgcctgg atgccagata atcaggttaa cggtgtcgat
     7801 tttgtcctgc ctttccgttt tgccgatgga gcctggcatc tggggactcg cggccccgtt
     7861 acgttgcgaa ttgccgaagt gattaatctg gtgacagcga aaaatattac ggctgatttg
     7921 caagggcgtt atccgtggac tgaagaagaa cccttgctgt tgactgatgt tagcgtcgat
     7981 gtgttaggcg gtaacgtact gatgaaacaa ttacgtatgc cgcaacatga cccggcgctg
     8041 ttgcggctga ataatctctc atccagcgaa ctggttagcg ccgtcaatcc gaaacaattc
     8101 gccatgtccg gggcatttag tggtgcattg ccattatggc taaacaacga aaaatggata
     8161 gtgaaagatg gttggctggc aaatagcggg ccaatgacct tgcggctgga taaagacacc
     8221 gcagatgcgg tggtgaaaga caatatgact gcgggttcag caattaactg gttgcgctat
     8281 atggaaatta gccggtcatc gacaaaaatt aacttagata atctcggttt attaaccatg
     8341 caggccaaca ttacaggtac cagtcgcgtt gatggtaaaa gcggtacggt aaaccttaat
     8401 taccatcatg aagagaatat ttttacgctg tggcgcagtt tacgctttgg cgataatctc
     8461 caggcatggt tggagcagaa cgcacgtctg ccgggaaatg actgtccgca aggaaaagag
     8521 tgtgaggaaa aacaatgaaa attttactgg ctgcgttgac gtcatctttt atgctggttg
     8581 gctgtacgcc tcgcattgaa gtcgctgcac ctaaggaacc gatcactatc aatatgaacg
     8641 ttaaaattga gcatgagatc atcatcaagg cagacaaaga tgtcgaagag ctgcttgaaa
     8701 ctcgtagcga tcttttctga ggcgatgatg aagaaaacat tacttctttg tgcgtttctt
     8761 gttgggctgg taagcagcaa tataatggca ttgactctgg atgaagccag aactcagggg
     8821 cgggtaggtg aaacatttta tggttatctg gttgcgctga aaacggatgc tgaaacagag
     8881 aaattagtaa ccgacattaa tgccgaacgt aaagcgagtt accaacaact ggcaaagcaa
     8941 aataatgtgt cggtagatga tatcgcgaaa ctcgccgggc aaaagctcgt agctcgggcc
     9001 aaaccggggg aatatgtaca agggataaac ggtaaatggg tgcgaaaatt ttaatagcaa
     9061 aaccgcaata tcgggataat gctacaggag aaagcgattg atctattttc ctgaaacaag
     9121 gtgaatatta aaaaactcct gttaaattgc cttttgccct gaaaaatgca tagtcaaata
     9181 tctgttttaa ctaattggcg ttgcagtaca tgcaacgcga attagttagc ggaatttacg
     9241 ccgatactcg cctggcgtca tcccaaagcg ttgcttaaat actgttgaaa aatgactctg
     9301 gtcggaaaat ccccaatgaa aaccaatgcc tgccagtttt tcatcatctg cggcatggcg
     9361 aatcgcatca gcacaaaaat cgagacgacg gttacgaata tattgcgcga ccaccaaacc
     9421 tttatcggca aacattcggt acaaactacg tactgacata cctgtctctc cggctatcca
     9481 ctccgggcgt aatatctctt cgcgaatatt atcgtctatc agcgtaacca ctttttgaaa
     9541 ctgacgttca cggcgaggtt gaacagattc ccgctgatga agtaccgggc gcagcagaca
     9601 caccatcgcc tgtagcgcag cttcactttc tgtttcagaa agtgccgggt tattcatgct
     9661 ctcctgtaac aggcgatgac tgagttgcac catgggtaag tcagcgtcca gtctttctgc
     9721 gcagacaggt ttttgatggg gaaaatattg ttccagcaga gtgcgtggta aaagtaatga
     9781 aatctgttta gaagactcct gccagtaaag cgaacagggg cgtgaggcat cgagtaacgt
     9841 aatatcgcca gcgccaatct gcacctgacg ctcatcctgc tccattattg cctgaccact
     9901 aagttgaaaa acggtgtaaa accaggcatc gtcgctgcct tttacctcct gccaggtgcg
     9961 gtataaattc actccgttgg ttgtcacggt gctcagcttt agtcctttgg caaaatgcgt
    10021 ttccagtaca cccgtgtaac gctcagtcag caggcgtccg gtaaaatttc cgcatacctg
    10081 attgatttgg gaaagccatt gctgaaactc attatccact gcggggttca tggcacgttt
    10141 tcgctctgtg aaatgtattt ttattgttgc atttgtgttg caataaacga agctaatgag
    10201 cctgactata ggaaataagt cttgtcaggc atagagacat aagcggttat tgtcacgatt
    10261 tgcggagctt gtcacagctg acaaagcgaa tgtcacagcg aaaaaagtga cttttcttgt
    10321 cgctgcgtac actgaaatca cactgggtaa ataataagga aaagtgatga cagagccgca
    10381 tgtagcagta ttaagccagg tccaacagtt tctcgatcgt caacacggtc tttatattga
    10441 tggtcgtcca ggccccgcac aaagtgaaaa acgattggcg atctttgatc cggccaccgg
    10501 gcaagaaatt gcgtctactg ctgatgccaa cgaagcggat gtagataacg cagtcatgtc
    10561 tgcctggcgg gcctttgtct cgcgtcgctg ggccgggcga ttacccgcag agcgtgaacg
    10621 tattctgcta cgttttgccg atctggtgga acagcacagt gaggagctgg cgcaactgga
    10681 aaccctggag caaggcaagt caattgccat ttcccgtgct tttgaagtgg gctgtacgct
    10741 gaactggatg cgttataccg ccgggttaac gaccaaaatc gcgggtaaaa cgctggactt
    10801 gtcgattccc ttaccccagg gggcgcgtta tcaggcctgg acgcgtaaag agccggttgg
    10861 cgtagtggcg ggaattgtgc catggaactt tccgttgatg attggtatgt ggaaggtgat
    10921 gccagcactg gcagcaggct gttcaatcgt gattaagcct tcggaaacca cgccactgac
    10981 gatgttgcgc gtggcggaac tggccagcga ggctggtatc cctgatggcg tttttaatgt
    11041 ggtcaccggg tcaggtgctg tatgcggcgc ggccctgacg tcacatcctc atgttgcgaa
    11101 aatcagtttt accggttcaa ccgcgacggg aaaaggtatt gccagaactg ctgctgatca
    11161 cttaacgcgt gtaacgctgg aactgggcgg taaaaacccg gcaattgtat taaaagatgc
    11221 tgatccgcaa tgggttattg aaggcttgat gaccggaagc ttcctgaatc aagggcaagt
    11281 atgcgccgcc agttcgcgaa tttatattga agcgccgttg tttgacacgc tggttagtgg
    11341 atttgagcag gcggtaaaat cgttgcaagt gggaccgggg atgtcacctg ttgcacagat
    11401 taaccctttg gtttctcgtg cgcactgcga caaagtgtgt tcattcctcg acgatgcgca
    11461 ggcacagcaa gcagagctga ttcgcgggtc gaatggacca gccggagagg ggtattatgt
    11521 tgcgccaacg ctggtggtaa atcccgatgc taaattgcgc ttaactcgtg aagaggtgtt
    11581 tggtccggtg gtaaacctgg tgcgagtagc ggatggagaa gaggcgttac aactggcaaa
    11641 cgacacggaa tatggcttaa ctgccagtgt ctggacgcaa aatctctccc aggctctgga
    11701 atatagcgat cgcttacagg cagggacggt gtgggtaaac agccatacct taattgacgc
    11761 taacttaccg tttggtggga tgaagcagtc aggaacgggc cgtgattttg gccccgactg
    11821 gctggacggt tggtgtgaaa ctaagtcggt gtgtgtacgg tattaatctg gttcgctcat
    11881 aagtaaaaaa cggcacctgg tgccgttttt ttgtctgaaa caatcactta tctttcttca
    11941 gcgcccctag cgttggcgtt tcgtcaaaga agttccatgg tttaagcaga gtatgtaccc
    12001 attcggtcgg cataatcggc cactcttcgg cgcgggccac atgtgtggtg ccggtggtca
    12061 tccagacaac ggcgtcggtg ttgtccagcg actcgttatc tttactgtat tgtccaagac
    12121 cggtgtcatg agtagaacgg ttcggatatt tgccttccgg gaaacgctcg ccaggatgat
    12181 aacgcgttac ccaaagctgc ttgtccataa agcttaaacg atgatagatc cactcgtccg
    12241 gcgcgaactg ggcacctttt gctaccgggt gagtaccacc tgcataagga ataatttgat
    12301 aggaaactgg attgcccatg cggttctctt tgttcgggtt actcaacaga cgaatcgtgc
    12361 ccggatcaaa cttctgcgcg gcatcctgtt cattgccgat gttgtactga ttaacttgca
    12421 tggtactggt gcgtgggcca ccggcagtat tcggttttac cactgggtcc atcgccacca
    12481 ggctgttatt ctcgccatct acatccagat cgaggcggaa attataaata tgttggtgtg
    12541 tagtacccac gatattgtga tcgataagcg tgccgtagcg cgtgtcatct ttcgccgtct
    12601 catcgtgcat ggttttcgct ttaacacctt tcaccgcttc gatgcccgta gcaccggcat
    12661 caatgccaat agtgccgttt tcatggaaga tccagtcaaa aatgtagtca tagttaccca
    12721 ctgtactgat ccagcgcacc actaactccc ggcgttcggt actgacgttg ggctggccca
    12781 tttcctgatg cttatactcc ggcccggcat aacgttcaaa taccgcgata gcgcgaggga
    12841 tctccatcgg cacgccagtg tagtcggcga tggtttcatt aaggagcact gcgttagacg
    12901 gggcatcttt accacgagca attggtgagg ttagcgtgcc cataccgtag tcaccagagt
    12961 ccagatacgc tttaaagtac cagccaatat caggatcacc gtaaggcaca atcatgccgc
    13021 cgagagaacc ttcgtacatg actttgcgtt tggtgccatt gtcgttataa gtcacggtgg
    13081 agatcatcgg cccgacgcga gagttcatgc tgaggtgaaa atcccagttc cgccagtgaa
    13141 tcatatcgcc agtaatggtg taatttttac cttcaggctc aatgatttgc ataggcttaa
    13201 ctgccggagc aacgcggtca cggctatcaa atgggcgtgc ggtcattggc accggaacta
    13261 ccggaccttc ttcaatctta acgatttttt tctgttctaa atcaacgacc gccaccaggt
    13321 tttcgatggg atgtgcccag tagttgccat caccgacatc aagatagctg atgactttga
    13381 gcaaccgggc atcttgtttc aggccatctt taccatcgaa ataacctacg gtcagcggcg
    13441 tggtaatcac ttttttcgca tcagtaatac cgcgtttctt cacggcagcg gcaaattctt
    13501 cactgttgtt aataatgttc tgcacactgg cgaaatcatc cagcaacacc ataccgtggg
    13561 cgtctttagt gggttgccag gagagcagtt tgttgttttg cagatccacc accgcttcga
    13621 tgatatgttt gccgtcgagc ataatgacgt cggctttgcg cggctggtca accggtttgt
    13681 tttccagcgc aaacgcccag acagcttctt tatctggcgg tagcagggag atctcagtaa
    13741 aacgggtatt gggtttgaag tccgcggaag ctttaacaat ttcaacggcc tgtttaattt
    13801 cgtccgcagt tagcgcatta agtgggtgag ggcgcttttc tacctgaaag gtttgatcca
    13861 gcccggactg gaaaacatcg ttaataaagg tgtcagaaac ccaggcttta ttgtctttca
    13921 tcactaccgg tacttgcagt gccagaggct gaccattaac aattgctgtt tgcgcaccag
    13981 gcttcacttt cacgtacgcg ccatctttaa tcagggtaaa gagctgggcg tagtcgtccc
    14041 actgcacatc ggcaccaaat tctttaagcg ttttatccat tggcaccata tgcgcttcac
    14101 caccgtgggc aaataccggc gcttgccagg cgaaacttaa ggcgactgcc aacgccaggg
    14161 ttgttttacg ggcagaatac agagaggggc ttcccattat taacctcgtc agatgttgtg
    14221 ttcttgttag caaccgcgct ctgtgggcgg tttagtcagg ttcacattat cagtactgat
    14281 gcaaagggga ttgcctgcac ctgccaggtt gtttggcagg tgtgccagct tttcatacag
    14341 tggatgccct gaaaatagat gtacacatca tgcataatgt gacaacgtca caaaatttag
    14401 tgaaataaaa gggcaactat tcgccgttgc ccttcattca ccgattaatc gacaaaatca
    14461 ccgtgctgcc tggccaccag cgtcagaatt gaatacagcg ccaccggggt ttgatgctga
    14521 ttgaatacct ctaccgccca ttccaccaca cctgttggtt tttcttctgc gctacgctgt
    14581 tttttcagcg tcttgcgctt acaggtgaga cgcacctgga tggtatcgcc tggctttacg
    14641 ggttcgataa aacgcaagct ttccagcccg tagttagcaa tgaccggacc aacaccggca
    14701 tcgacaaaca gacccgcagc cgcagaaagc acaaaatacc catgcaccac ccgctcaccg
    14761 aaaatagatt cggcagcagc aatcttatcc atatgtgcat agaaatgatc gccgctgagg
    14821 caagcaaagt taacaatatc ggcctctgtc attgtgcggc ggggagtcaa caggctgtcg
    14881 cctggttgta gctcctcaaa atatttgcgg aacggatgaa tacgatcttc ttcgactttc
    14941 gcaccgcgca cccactgttt actgatagcg gcaagcatcg tcggactacc ctgaacagcg
    15001 gttcgctgca tgtaatgttt caccgctcgt aaaccgccta attcttcacc gcctcctgcg
    15061 cgaccaggcc caccatgtac cagttgtggc agtggggagc catgcccggt ggattctttt
    15121 gccgattctt cattgaggat ctgaattcgc ccatgcgtac gtgccgcgtc ggcaataaac
    15181 tgacgcgcaa tttgcggatc agccgtcacc agcgttcccg caaggctacc gccgcctgca
    15241 caagccagtt gcagagcatg ttgctggttt tgtgctggca ttagcgttgc gacagggcca
    15301 aaggcttctg ttgcatgtac cgccggtgtt tcatccggct gcggacagta caataaggtt
    15361 ggcgggaaga aggcacccgc cgcagataaa tccgcctgac caccgaggcg aatctcgcat
    15421 cctgcagcca gcaatgtgtt cactttttcc tgcacatcag cacgctgttc agcatttacc
    15481 agtgcgccca ttttcacgcc ttcttgtgca ggatcaccga ccacgacttt ctgtaatcgc
    15541 gcaaccagag catcactgac agcattaacc aatgtctgcg gcacaataat ccgccggatt
    15601 gccgtacatt tttgcccggc ttttgtggtc atctcacgca caacttcacg aataaacagc
    15661 gcaaactccg gttgatccgg ggtgatatct tcgcccagta cgcagcaatt cagggaatca
    15721 gcttccatgg tgaaggggat agatttggct acgatatttg gctgaactcg tagcatctgt
    15781 ccggtcgtcg cggaccccgt gaaagtcacc acatcctggc tgtccagatg atccaaaagg
    15841 tcgcccgcac taccgcagat cagactaatt gcgccttcgg gaacaagacc actatcgaca
    15901 attgatttca ccatcgcctg agtcagttgg gccgtcgcgg tagctggttt gatgatggct
    15961 ggcattccgc ccagccacgt tggtgccagc ttttccagca ttccccagca ggggaagtta
    16021 aaggcgttaa tatgcactgc cacgcctgac tttgaggtca gtacatggcg cgcggcaaat
    16081 ccaccttctt tcgataaggg gagcaattca tcttccggcc acagcgtatc gtcaggcagc
    16141 tcccggctac cgaggctggc gtaagtaaat aacgtcccaa tgccaccttc aatatcaacc
    16201 caactgtctg cccgcgttgc gcctgtttgc gcagaaagag catagaaacg ctctttttca
    16261 ctcagcagat gtttagcgac cgctttaagc atcgccgcac gttcgataaa ggtcatagcg
    16321 cgaagggctg gggcaccttt ttcaatggca aacaggcggg cagccgccat atcaagacct
    16381 tcactggtca cttcccataa cgcctcgccg ctaatagcgt ggtgaatcaa acggctacgg
    16441 ccccggccag actgccaggt accggataag aaactggcta actgctgcat cgctactctc
    16501 cagatgtttc acatttctgt tgctaatagt tgaatcgcga atcataaaaa gcaagggatc
    16561 ttttaatgaa atgttaacta tgcgatctgt atagcaactg cggaaaacat taatgcaatg
    16621 ataaataatg atttataaaa agagggtgcg aaatccgtca cagttcaagc atacaaaatt
    16681 tgtgatttta gttaaccatt gtgtaacttt cataaaacaa tgtgattcgt gtttttaatt
    16741 aattcacgaa aactggaatc gtaaaggtga tgacgtgacc caagaagaac gctttgagca
    16801 acggatagcc caggaaacgg ctatcgagcc acaggactgg atgcccgatg cttaccgaaa
    16861 gacattgatc cgccagattg ggcagcatgc gcactccgag attgttggta tgttgcctga
    16921 aggtaactgg atcactcgcg caccaacctt gcggcgtaaa gccattctgt tagccaaagt
    16981 gcaggatgaa gccggtcatg gtctgtatct ctatagcgcc gccgaaacac tgggctgtgc
    17041 cagagaagac atctaccaaa aaatgctcga cggacggatg aaatattcct ccatctttaa
    17101 ttatccgaca ttgagttggg ctgatatcgg cgttatcggc tggctggtgg atggcgcagc
    17161 gatcgtcaat caggtggcgt tatgccgaac ttcttatggc ccgtatgcca gagcaatggt
    17221 gaaaatctgc aaagaagaga gttttcacca gcgtcagggg tttgaagcct gcatggcact
    17281 ggcgcagggc agcgaagctc aaaagcagat gttgcaagac gccattaacc gtttctggtg
    17341 gccagcctta atgatgttcg ggccaaacga tgataactcg ccaaacagcg ccagaagtct
    17401 cgcctggaaa atcaaacgtt tcaccaacga cgaactccgc cagcgtttcg tggacaacac
    17461 cgttccacag gttgaaatgc tcggtatgac cgttcctgac ccggatctgc attttgacac
    17521 tgaaagcggt cactaccgct ttggtgagat cgactggcag gagtttaacg aagtaattaa
    17581 cggtcgcgga atttgtaatc aggagcggct tgacgccaaa cgtaaagcct ggaaagaagg
    17641 tacctgggta cgggaagcag cgctggccca tgcacaaaaa caacatgccc gtaaggtcgc
    17701 ataaggagat tcaaaatgag taatgtttac tggccgttat acgaagtttt cgtgcgtggc
    17761 aaacagggct tatcacaccg ccatgttggc agtttacatg ctgccgatga gcggatggca
    17821 ctggaaaatg cccgtgatgc ttacacccgt cgtagcgaag gatgttcaat ttgggtggtg
    17881 aaggcgagtg aaattgttgc ctcgcaaccg gaagaacgcg gtgaattttt tgatccggct
    17941 gaaagcaagg tctatcgcca tccaacgttt tacaccatcc ctgatggcat tgagcacatg
    18001 tgaggtcgga aatgaatcag ttaacggctt acaccttgcg cctgggcgat aactgcctgg
    18061 tgctctccca gcggttgggt gaatggtgcg gtcatgcacc ggaactggaa atcgatctcg
    18121 cactggcaaa tattggactc gatttattag gccaggcacg caacttctta tcgtatgccg
    18181 ctgaattagc gggagaaggc gatgaagata ccctggcctt cacccgagac gagcgccagt
    18241 tcagcaactt attgttggtt gaacagccaa acggcaattt tgccgacacc attgcacgcc
    18301 agtatttcat cgatgcatgg catgtggcgc tctttacccg tctgatggag agccgtgatc
    18361 cgcaactggc ggcgatttct gccaaagcaa ttaaagaagc gcgttatcac ctgcgtttta
    18421 gtcgtggctg gctggagcga ctgggcaatg gtactgacgt atcagggcaa aagatgcagc
    18481 aggccatcaa caagttgtgg cgttttaccg ccgaactgtt cgatgccgac gagattgata
    18541 ttgcactgag tgaagagggt attgcggttg atccacgcac tttacgcgca gcgtgggaag
    18601 ccgaagtttt tgccgggatc aacgaagcca cattgaacgt accgcaagag caggcgtatc
    18661 gcactggcgg taaaaaagga ctgcataccg aacaccttgg acccatgctg gcagaaatgc
    18721 agtatctcca gcgtgtcttg cccggtcagc aatggtaaca gaggagatgg gtatgcaacg
    18781 tctggccacc attgcaccgc cacaggttca tgagatatgg gcgctactca gccagatccc
    18841 ggacccggag atcccggtgc tgaccattac cgatttaggc atggtgcgta atgtgacaca
    18901 gatgggagaa ggatgggtga tcggctttac gccgacttat tccggttgtc cggcaacgga
    18961 acatttgatt ggcgcgatac gtgaggcaat gacaacccac gggtttaccc ccgttcaggt
    19021 tgtgctgcaa ctcgacccgg catggaccac cgactggatg acccccgatg cccgtgagcg
    19081 tctgcgacag tatggcatta gcccgcccgc cggacacagt tgccatgccc atttgccgcc
    19141 agaagtacgt tgcccgcgct gcgccagcgt ccataccaca cttatcagtg aatttggttc
    19201 cacggcctgc aaagcattgt atcgctgcga tagttgccgc gaacctttcg attatttcaa
    19261 atgtatttga ggatgccatg acaacgtttc attccttaac ggtggcaaaa gtggagccgg
    19321 aaacccgtga tgcggtgacc attacctttg cggtgcccca gcctttgcag gaggcgtatc
    19381 gctttcgccc cggtcaacat ttgaccttaa aagccagctt tgatggtgaa gaattacgcc
    19441 gttgttactc catttgccgc agctatctgc ctggcgaaat tagtgtggcg gtgaaagcca
    19501 ttgaaggcgg acgtttctcc cgctatgccc gcgaacacat ccgccagggt atgacgctgg
    19561 aggtcatggt gccgcagggg catttcggct atcagccaca ggccgaacgc cagggccgct
    19621 atctggctat tgcagcagga tcaggtatta cgccaatgct ggcgattatc gccaccactt
    19681 tacaaaccga gcctgaaagt cagttcaccc tgatctacgg taaccgtacc agccagagca
    19741 tgatgtttcg ccaggcactg gcagacctga aagacaaata tcctcagcgt ttacagttgt
    19801 tgtgcatttt cagtcaggaa accctcgaca gcgatctgct tcacgggcgt attgacggtg
    19861 aaaaattaca gtcacttggg gcctcgctca ttaattttcg tctttatgat gaggcattta
    19921 tttgtggtcc ggcggcgatg atggatgaga cggaagccgc cttaaaagca ctgggaatgc
    19981 cagataaaac cattcatctg gagcggttta atacgcctgg cacgcgcgtc aaacgtagcg
    20041 ttaacgtgca aagtgacgga caaaaagtga ctgtacgtca ggatgggcgg gatcgggaaa
    20101 tcgtgcttaa tgccgacgat gaaagcattc tcgatgcggc attgcgccag ggggctgatc
    20161 tgccctatgc ctgcaaaggc ggcgtctgtg cgacctgcaa atgcaaagtg ctgcgtggca
    20221 aagtggcgat ggaaaccaat tacagtctgg aaccggatga actggccgca ggctatgtgt
    20281 tgagttgcca ggcactgccg ctgaccagcg atgtggtggt tgactttgac gcgaagggga
    20341 tggcatgagc gaactgatcg tcagccgtca gcaacaagta ttgttgctga cccttaaccg
    20401 tcccgccgca cgtaatgcgc taaataatgc ccttctgacg caactggtaa atgaactgga
    20461 agctgcggct accgatagca gcatttcggt ctgtgtgatt accggtaatg cacgcttttt
    20521 tgccgctggg gccgatctca acgaaatggc agaaaaagat ctcgcggcca ccttaaacga
    20581 tacacgcccg cagctatggg cgcgattgca ggccttcaac aaacctctca tcgcagccgt
    20641 caacggttac gcgcttggtg cgggttgcga actggcattg ttgtgcgatg tggtggttgc
    20701 cggagagaac gcgcgttttg gtttgccgga aatcactctc ggcatcatgc caggcgcagg
    20761 aggaacgcaa cgtttaatcc gtagtgtcgg taaatcgtta gccagcaaaa tggtgctgag
    20821 cggagaaagt atcaccgctc agcaagcaca gcaggccggg ctggttagcg acgtcttccc
    20881 cagcgattta accctcgaat acgccttaca gctggcgtcg aaaatggcac gtcactcgcc
    20941 gctggcctta caagcggcaa agcaagcgct gcgccagtcg caggaagtgg ctttgcaagc
    21001 cggacttgcc caggagcgac agttattcac cttgctggcg gcaacagaag atcgtcatga
    21061 aggcatctcc gctttcttac aaaaacgcac gcccgacttt aaaggacgct aatgatggaa
    21121 ttcatcctca gtcatgtaga aaagggcgtg atgacactaa cgctcaaccg cccggaacgc
    21181 ctgaacagtt ttaatgatga gatgcacgca caactggcag agtgcctgaa acaggtcgag
    21241 cgcgacgaca ctatccgttg cctgttactt acaggtgccg ggcgcggatt ttgtgctggt
    21301 caggatctta acgatcgtaa cgttgatccc acaggccccg caccggattt agggatgtca
    21361 gttgaacgtt tctataaccc actggtacgt cgcctggcaa aactgccaaa accggtgatc
    21421 tgtgcagtca atggcgtggc ggcaggggct ggcgcaacac tggcactggg atgcgacatc
    21481 gttattgctg cccgttcagc aaaattcgtc atggcgttta gtaagttagg tttagtaccc
    21541 gattgcggtg gaacctggtt actgccacgc gttgctgggc gagcgcgcgc catggggctg
    21601 gcactgctgg ggaatcaact gagtgctgaa caggcgcacg aatgggggat gatctggcag
    21661 gttgttgatg atgaaacgct ggcagatacc gcgcaacagc tggcacggca tctggcgaca
    21721 caaccgacat ttggtcttgg acttatcaag caagcgataa atagcgctga aaccaatact
    21781 ctcgatacgc aactggatct ggaatgcgac tatcagcgac ttgccggacg gagcgcagat
    21841 tatcgtgaag gtgtcagtgc gttcctggct aaacgctcac cgcagttcac ggggaaatag
    21901 catgatgata aatgtgcaaa ctgtggcagt gattgggagc ggcaccatgg gggctggcat
    21961 tgctgaagtt gccgccagtc atggacatca ggttttactg tatgacattt ctgctgaagc
    22021 gctgacccgc gcaatcgacg ggatacacgc gcggctaaat tcacgcgtga cgcggggaaa
    22081 actgactgct gaaacctgtg aacgcacatt gaaacgcctg atcccggtga ccgatattca
    22141 cgcgctggca gctgcggacc tggtcattga agcggcgtct gaacgtctgg aagtcaaaaa
    22201 agcgctcttt gcacagctgg cggaagtttg cccgccacaa acgctattga ccactaacac
    22261 ttcgtcaatc tctataaccg cgattgctgc ggaggtaaaa aatcctgaac gtgttgcggg
    22321 gctgcatttt tttaacccgg caccggtgat gaagttggtg gaggtggtca gtgggctggc
    22381 aacggcagcg gaagttgttg agcagttgtg tgaactaacg ttgagttggg gtaagcagcc
    22441 tgtgcgctgt cattcgaccc ctggatttat cgttaaccgt gtggcgcgtc cttattattc
    22501 cgaggcctgg cgggcactgg aagagcaggt tgctgcacca gaagtgattg acgctgcact
    22561 tcgcgatggc gctggtttcc cgatggggcc gctggaatta accgatctga ttggtcagga
    22621 cgtcaatttt gctgtcacct gttcggtgtt taacgctttc tggcaggagc gtcgtttttt
    22681 accttcgctg gtgcaacagg aactggtgat tggtggacgg ttgggcaaga aaagtgggct
    22741 gggcgtgtac gactggcgcg cggaacgtga ggcagttgtt ggcctggaag cggtaagcga
    22801 cagttttagc ccaatgaaag tagaaaagaa aagtgacggt gtcacggaaa ttgacgatgt
    22861 tttattgatt gagacacaag gcgagacggc acaggcgctg gcaatacgac tggcacgccc
    22921 ggtggtagtg gtcgacaaaa tggcgggcaa ggtggtgacc attgctgcgg cagcggtgaa
    22981 cccggactca gcgacccgca aggccattta ttacctgcaa cagcagggca aaacagtgct
    23041 gcaaattgca gattacccag gaatgctgat ttggcgaacg gtagcaatga tcatcaatga
    23101 agcccttgat gcgctgcaaa aaggcgtggc ctctgaacag gatatcgata ccgccatgcg
    23161 tcttggggtg aattatccat atggcccgct tgcctgggga gcgcaacttg gctggcagcg
    23221 aatattaagg ctcctggaaa atctacaaca tcactatggc gaagaacgct atcgcccatg
    23281 ttcattgctg cgccaacgag cgcttctgga gagcggttat gagtcataag gcctggcaaa
    23341 atgcccatgc aatgtatgag aacgatgcct gcgccaaagc gcttggcatc gacattatct
    23401 caatggatga aggctttgct gtagtgacca tgaccgtcac tgcacaaatg cttaatggtc
    23461 atcaaagttg ccacggcggg cagctatttt cactggctga tactgccttt gcctacgctt
    23521 gcaatagcca ggggttggca gctgtcgctt ctgcctgcac gattgatttt ttacgtccag
    23581 gctttgccgg agacacctta actgcgactg cgcaggtacg tcatcagggc aagcaaaccg
    23641 gtgtttacga catcgaaatt gttaaccaac aacaaaaaac ggttgcgctg tttcgcggta
    23701 aatctcaccg catcggcggc accattacag gagaagcctg atgcgtgaag cctttatctg
    23761 tgacggaatt cgtacgccaa ttggtcgcta cggcggggca ttatcaggtg ttcgggctga
    23821 cgatctggct gctatccctt tgcgggagct gctggtgcga aacccgcgtc tcgatgcgga
    23881 gtgtatcgat gatgtgatcc tcggctgtgc taatcaggcg ggagaagaca accgtaacgt
    23941 agcccggatg gcgactttac tggcggggct gccgcagagt gtttccggca caaccattaa
    24001 ccgcttgtgt ggttccgggc tggacgcact ggggttcgcc gcacgggcga ttaaagcggg
    24061 cgatggcgat ttgctgatcg ccggtggcgt ggagtcaatg tctcgggcac cgtttgttat
    24121 gggcaaggca accagtgcat tttctcgtca ggctgagatg tttgatacca ctattggctg
    24181 gcgatttgtg aacccgctca tggctcaaca atttggaact gacagcatgc cggaaacggc
    24241 agagaatgta gctgaactgt taaaaatctc gcgagaagat caagatagtt ttgcgctacg
    24301 cagtcagcaa cgtacggcaa aagcgcaatc ctcaggcatt ctggctgagg agattgttcc
    24361 ggttgtgttg aaaaacaaga aaggcgttgt aacagaaata caacatgatg agcatctgcg
    24421 cccggaaacg acgctggaac agttacgtgg gttaaaagca ccatttcgtg ccaatggggt
    24481 gattaccgca ggcaatgcct ccggggtgaa tgacggagcc gctgcgttga ttattgccag
    24541 tgaacagatg gcagcagcgc aaggactgac gccgcgagcg cgtatcgtag ccatggcaac
    24601 cgccggggtg gaaccgcgcc tgatggggct tggtccggtg cctgcaactc gccgggtgct
    24661 ggaacgtgcg gggctgagta ttcacgatat ggacgtgatt gaactgaacg aagcgttcgc
    24721 ggcccaggcg ttgggtgtac tacgcgaatt ggggctgcct gatgatgccc cacatgttaa
    24781 ccccaacgga ggcgctatcg ccttaggcca tccgttggga atgagtggtg cccgcctggc
    24841 actggctgcc agccatgagc tgcatcggcg taacggtcgt tacgcattat gcaccatgtg
    24901 catcggtgtc ggtcagggca tcgccatgat tctggagcgt gtttgagcat atcaacctgc
    24961 gagtacccta caatgataac caatacaaag cttgacccga ttgaaaccgc gtctgttgat
    25021 gagttacagg cgttgcaaac acagcgtctg aaatggacgc tcaaacacgc gtatgaaaat
    25081 gtcccgatgt atcggcgcaa attcgacgca gcaggcgtac atcctgatga tttcagggaa
    25141 ctttcagacc tgcgtaaatt tccctgtacc accaaacagg atctgcgcga caactatccc
    25201 tttgacacct ttgccgtgcc gatggaacaa gtggtgcgca ttcatgcttc ttcgggaacc
    25261 acaggtaaac cgacagttgt cggttatacg caaaacgata ttgataactg ggccaatatt
    25321 gtagcgcgtt ctttgcgtgc ggcagggggc tcgcccaaag acaaaattca tgttgcctat
    25381 ggttacggcc tgtttactgg tgggctgggc gcgcactatg gtgccgaacg tctgggcgct
    25441 acggtgatcc cgatgtctgg cgggcagacg gaaaaacaag cgcaactgat ccgcgatttt
    25501 caaccagata tgatcatggt tacgccatct tattgcctta acctgattga agagctggag
    25561 cggcagttgg gcggtgatgc cagcggttgc tcgctgcggg ttggcgtgtt tggtgctgag
    25621 ccgtggacac aggccatgcg taaagagatt gaacggcgtc tggggatcac cgcactggat
    25681 atttatggcc tgtcagaagt gatggggccg ggggtggcga tggagtgtct ggaaactacc
    25741 gacggtccaa ctatttggga agatcatttc tatcctgaaa ttgttaatcc tcatgacggc
    25801 acaccgcttg ccgatggtga acatggcgaa ctgttattca ccacgctgac caaagaagca
    25861 ttgccggtca ttcgttaccg cacgcgtgat ttaacccgac tgttaccagg aacggcgcgg
    25921 actatgcgcc gcatggatcg catcagcgga cgcagcgatg acatgttgat tattcgcggt
    25981 gtaaatgtct ttccgtcaca actggaagaa gagattgtca aattcgaaca tttatcgccg
    26041 cattaccaac tggaggtgaa ccgccggggg catcttgatt cactttctgt gaaagtggag
    26101 ttgaaagaaa gtagcctgac attgacgcat gagcagcgtt gccaggtatg ccaccagctg
    26161 cgccatcgga ttaagtcgat ggtggggatc tctaccgatg tgatgatcgt taactgtggc
    26221 agtatcccgc gttcagaagg caaggcgtgt cgggtgtttg atctgcgcaa tattgttggt
    26281 gcctgacgtg tcgtctggcc ctggtgaggt aaagcgccag ggccagaagt cgatacgacc
    26341 tgtgctatga ttcataaatc acaacaataa caacagactg aatcgaatga gtaaacttga
    26401 tacttttatc caacatgctg taaacgctgt tcctgtcagt ggcacatctt tgatctcctc
    26461 tctgtatggt gattctcttt cccatcgtgg tggtgaaatc tggctgggta gtctggctgc
    26521 tttgctggaa gggctgggat ttggtgagcg tttcgtgcgc actgctttgt ttcgtcttaa
    26581 taaagaaggc tggctggatg tttcccgcat cgggcgacgc agtttctata gcctcagtga
    26641 taaaggcttg cgcctgacgc gacgggcaga aagtaaaatt tatcgcgcag agcaacccgc
    26701 atgggatggt aaatggctcc tgttgctctc ggaaggttta gataaagcga cgctggctga
    26761 tgtcaaaaag cagttgatct ggcaaggttt tggtgcactg gcgcccagcc tgatggcatc
    26821 gccgtcgcaa aaactggccg atgtacagac acttttgcat gaagcgggtg tggcggataa
    26881 cgtgatttgt tttgaagcgc aaataccact ggcgctttct cgtgcagcac tgcgtgccag
    26941 agtagaagag tgctggcatt taactgaaca aaatgccatg tacgaaacct ttattcagtc
    27001 attccgcccg ttggtgcagc tgttaaaaga ggcagcagac gagttaaccc cggagcgtgc
    27061 atttcatatt cagcttttac tgatccattt ttatcgccgt gtcgtcctta aagacccttt
    27121 gttgccggag gagttgcttc cggctcactg ggcagggcat acggcgcgtc agctgtgtat
    27181 caacatttat cagcgcgtag cgcctgctgc tttagcgttc gttagtgaaa aaggtgaaac
    27241 ctcggtcggt gaactgcctt cgccgggtag cctgtatttt caacgttttg gcggtttgaa
    27301 tattgaacag gaggcgttat gccaatttat cagatagacg gtctgactcc ggttgtgcca
    27361 gaagagagtt ttgtccatcc gacagcggta ttgatcggcg atgttattct cggcaagggt
    27421 gtttacgttg ggccaaatgc cagcctgcgt ggcgattttg gtcgtatcgt ggtgaaagat
    27481 ggcgcgaaca ttcaggataa ttgcgttatg cacggttttc ccgagcagga tactgtcgtt
    27541 gaagaagatg ggcatatggg tcatagcgct atccttcacg gctgcattat ccgccgcaat
    27601 gcattagtgg gaatgaacgc ggtagtgatg gatggtgcgg tgattggcga gaacagcatt
    27661 gttggcgcat ccgcatttgt gaaagccaaa gcagaaatgc ctgccaatta cctgattgtc
    27721 ggcagcccgg cgaaactgat tcgtgagctt agtgagcagg agctggcatg gaaaaagcag
    27781 ggtacgcatg agtatcaggt gctggtgaca cgctgtaagc agacgttaca tcaagtcgaa
    27841 ccattgcggg aagttgaacc tggcaggaaa cgcctggtat ttgatgagaa tctgcgaccg
    27901 aaacagtaac gtccagatac tgaacgttct aatttttagc atgtgatacc taaaccgcaa
    27961 cgctgataca ggttgcggtt tttttattgc cgaatgcggc gctttacgcg ttttgttttg
    28021 gtgtctttca ggacaataga gcaactcatc cagtaatctt gtttacgcct tcgcattatt
    28081 tatctctttt cgtttctata ctgatttttc tcaatccgtt ttattacagg gcagggtgcg
    28141 atgagcagca atacatttac tctcggtaca aaatccgtta accgtcttgg ttatggcgcg
    28201 atgcaactgg caggtcctgg ggtttttggt cccccaagag atcgccacgt cgctataacc
    28261 gtgctgcgcg aggcgctggc attgggcgtc aatcacattg ataccagcga cttttatggt
    28321 ccgcacgtca ccaatcagat tatccgcgaa gcgctttatc cttactctga cgacctgaca
    28381 attgtcacta aaattggtgc gcggcgtgga gaggacgcat cctggttgcc cgcattttct
    28441 ccggcagagc tgcaaaaagc ggtgcacgat aatctacgta atctcgggct ggacgtactg
    28501 gatgtggtta acttgcgcat tatgatgggg gatggtcatg gcccagcgga aggatcgatt
    28561 gaagccagcc tgaccgtgct ggcagagatg caacaacaag gcctggtaaa acatattggc
    28621 ctaagcaacg tcacaccgac gcaggttgca gaggcgcgca agattgccga aatcgtctgt
    28681 gtgcagaacg aatacaacat tgctcatcgt gctgatgacg caatgattga tgctttggct
    28741 cgtgacggca ttgcctacgt gccgttcttc ccgctcgggg gctttacacc gctgcaatcc
    28801 tctacgctgt cggatgttgc tgcgagcctg ggtgcaacac ccatgcaggt ggcgctggcg
    28861 tggctgttac aacgttcacc gaatattttg ctgatcccag ggacgtcttc tgttgcgcat
    28921 ttacgggaga atatggctgc tgaaaaattg catctttctg aggaagtgtt gtctacgttg
    28981 gatggtattt cgcgagaata acgaatatac aaaagggaaa gatgcatttc ccttttgttt
    29041 cttttttaat ggcatggagt gcatatgttt aaggccagga tttgcggctg gataggttta
    29101 cttcccctgt ttatgctgag tcttccagta caggcggaac ttcgatgtgt tgcaaataca
    29161 gttgatattg aaccattttt ttctgcggct accgctgaag ataaacaaca agttgaacaa
    29221 gctatcaaca gtagcgtgaa tcttgtcccc ttcggtttat ctgcatcgga ctggaaagtg
    29281 catcgtggcg atttagtggt agaaggtaat atagagagta atcaaaaatt gattgttctt
    29341 ggtaatttga cagtcaaagg taatatttcc actttctctc tttctaatcc atgggttatt
    29401 ctcggtaacg tgacggcaac gaatatagtt acggattcac cactattaat taccggttcg
    29461 ataaatgcaa gtggactggt atttatcgac tcatattacg ataatccgtc ttcgattaag
    29521 gggagtatta atgcgcgtgg aatatttatc aatgacataa ttgcacctgt tgttgcgtct
    29581 tcgacaaata gtgaattcat ggtccgtgcg agtgacaaaa atgacactga aaatgtcaaa
    29641 aaagcgctga tgataataaa tcctgatgca tattattggg gtctaataaa tgatgaagat
    29701 gctctgaaag aaatttttaa gcgaagcaat attcgcatgg cagggaatgt ctgtaatcag
    29761 atgaaaaaag aagcgctgtt tcgccctaag ccttctcctg agttagtgca ggaattgcaa
    29821 atgctggatg aaggcaacgt tgctgcattt gaaggacgag atattgcgac atttgatctt
    29881 gccataatgc gaactctccc aaggcttaaa ggaatttcag ctaacctacg caaacaactt
    29941 attaatagca atgatgaaca aacgattgaa agtatggcga gatatatgcc tgacaatgag
    30001 attctggagt tgaccgatca gcaattgggt taccagcctg ttgttctggg gttgctcgac
    30061 cgtgaaccgc tctctgtcga aataatgacg cgaatgagtc gtctacctga tggtgtcggt
    30121 ccgttgaatc ttgcgctacg tgaaaatctc cctctggata tcgtaatgac tctggcgaaa
    30181 agagattggg atatgatcat tcaggagctt tataaagatg cgtggttatt gcctgaatcc
    30241 attattgatg gctatatccg cagtgatgat tcctctattc gtcaggtcgg tgctggagga
    30301 caactcacct acaatcaggc aatgcagttg gcgaacgatt catcaaacaa tgttgtcaca
    30361 agcttagcgt tcaagctggc agagatgaaa caccatggtc aattattgcg gatgacgcca
    30421 caagagagtg acaaagttgc agcctattta taccaaaaat tcgagaatga cgatgatcta
    30481 atacgtgtgt tatttttagc attgccagat aacttacagt ttaattttgt taaaaggatg
    30541 gagaaaaaat cccctgccta cttttgctgt cgggatatgc aggtaattca ctctgacgct
    30601 gctctacaac ggttattgac acgtttcaac gatcctgagg ggtggagtaa tctggcgaaa
    30661 aatcagtatc tgagcacgtc gatgaaacaa aaaatttggc aacgtgcttt atcgcatcgg
    30721 aaaaataatc cgaaagcaga ttcagctgcg tatgaaacca gcgctgatat gattttgtct
    30781 gagcttatca gccacggtga agttgatgat cagatgctat taaatgccac cgccctgata
    30841 cgtttggaag actgggactt tttagagagt gcattagtta gttgggataa tttacccgct
    30901 gttgtcctca aggaattaca gcaaaatacg ccacgcaatg atatttgggc gaagtttttt
    30961 ctgaggcagg aaaacagctc ccgtgcgcag gttgatgaag cattacgcgt ctattatgca
    31021 ctcgaccccg atgcgttagc acaactggat gtactggcaa aacaaccgga tcgtatatgg
    31081 tggagtacac tggcgaaaag caatctcaca tttttcaagt tcggcgcact taacaaccgc
    31141 cacacaccgc ctgcagtact ggcggcagaa attgatcccg agtggtggat tgtggcgatg
    31201 aataatcccc gttttccagt tgatgtatta aaggcgaggc tgaaacgtga tcctttgctg
    31261 gcgttagagc ttgttaaccc tgaactggat ttagtccgcc agctggcgct taacggtaag
    31321 acgcgcgcaa tacgggaaca agcgatgaga aaacttgatg agttgtattg atccacaacg
    31381 taatatttct taaccctttg attaattggg gataattatt cctgacagtg agtccccaat
    31441 accttgatat attctgaatt tttaatgaag cggcgtgttg cgatatctcc gtcaggggaa
    31501 ttgatgcacc atagcgcaaa ccgaattatc aaggattgat aatgacgctc taccagataa
    31561 aaccgctctt tcagtcgctg ttaaggccga cgatgttttg gctttacaag caccacgtta
    31621 cagcgaatca catcactctt gctgcactgg cgctttctct tcttaccgga ttgctgttga
    31681 tgttggtggc acaacccatc ctctttatgc tattgcccat cgtgcttttt atccgtatgg
    31741 cgctcaatgc gctggatggc atgttggcgc gtgagtgcaa tcagcaaaca cggcttgggg
    31801 caattttgaa tgagactggt gatgttattt ccgatattgc gctctattta ccatttttat
    31861 ttttaccgga aagtaacgca tcactcgtga tactcatgtt attttgcacc atattgaccg
    31921 agttttgcgg tttactcgcg cagacgatta acggtgttcg cagttatgcc ggtccatttg
    31981 gcaaaagcga tcgcgcgtta atatttggtc tgtggggtct ggccgttgcc atttatccac
    32041 agtggatgca gtggaataat cttttatgga gcattgcttc aattctgctt ctctggactg
    32101 cgattaatcg ttgtcggagt gtgcttctta tgagcgctga aagataatgc tggaaaaatc
    32161 tctggcaaca cttttcgcgt tgttaatttt agcaacgctg ataaaccatt ttctggtatg
    32221 gcggttaccg gagagaaaag aggatgaagt tacattacgt attcgtacct ggtggggcat
    32281 cgtcatttgt ttttcactgg tgatttcagg tccacgctgg atgacgttga cgttttttgc
    32341 gctgataagt tttctggcat tgaaagaata ttgtacgctt atatctgtac attttccgcg
    32401 gtggttatat tgggttattc ctcttaacta tttgtttatc ggttttaatt gctttgagct
    32461 gtatctatta tttatacctt tggctgggtt tctaatatta gccaccgggc aagtttttgt
    32521 gggggacccc tccggttttc tgcataccgt gagcgccatt ttttggggct ggataatgac
    32581 cgttttcgcc ttgagtcatg ccgcctggtt attaatgttg ccaaccacaa atatccaggg
    32641 cggggcgtta ctggtgttat ttcttttggc attaaccgag tcaaacgata ttgcacagta
    32701 tttatgggga aaatcctgcg gcagaagaaa agtggtcccc aaagtcagcc cgggaaaaac
    32761 gctggaaggt ctggtggggg gcgtcatcac caccatgatt gcgtcactga ttattggacc
    32821 gttgctgaca ccgctaaata cattacaggc attattagcg ggtttgttaa ttggtattag
    32881 tggtttttgc ggcgatgtcg tgatgtcagc catcaaacga gatattggtg ttaaagatag
    32941 tggaaaacta ttaccaggac atggcggact ccttgacagg attgactcat taattttcac
    33001 cgctccggta tttttttatt ttattcgcta cagctgttac tgaaggaaaa tagagaaaat
    33061 ggaaaattca cgcatccctg gggaacattt ttttaccacc agtgataata cagcgttgtt
    33121 ttatcggcac tggcccgctt tacagcccgg ggcgaaaaag gtcatcgtct tatttcatcg
    33181 gggtcatgaa cattctggtc gtctacaaca tctcgttgat gaactggcga tgccagatac
    33241 tgctttttat gcatgggatg cccgggggca tggaaaaact tcggggccgc gtggttatag
    33301 cccatctctt gcgcgttcag tgcaggatgt cgatgaattt gtccgttttg ctgccagcga
    33361 cagccaggtt ggactggaag aggtggttgt gatcgcgcaa agcgtcggcg cagtgctggt
    33421 tgccacatgg gttcatgatt atgcgcctgc aattcgcggg ctggtgctgg cttctccggc
    33481 ctttaaggtt aaattgtatg tgccgctggc acgtcctgcg ctggcgttat ggcatcgtct
    33541 gcgtggtctg ttttttatta attcctatgt gaaaggacgc tatttgaccc acgatcggca
    33601 acgggtggcg agtttcaata atgatccgct gatcacacgg gcgattgccg tcaatatctt
    33661 gctcgatctc tacaaaacgt ctgaacgtat tattagagat gcggcggcga ttacgctccc
    33721 cacgcaactt ctgatatcag gcgatgacta tgtggtgcat cgccaaccgc agattgattt
    33781 ttatcagaga ttacgtagcc ctctgaaaga gctgcatctg ctgccaggct tttatcacga
    33841 cacgttgggt gaagagaaca gggcgcaggc atttgaaaaa atgcaaagct ttattagtcg
    33901 tttatatgct aacaagtcac aaaaatttga ttatcagcat gaagaccgca ctggaccatc
    33961 agcggatcgc tggcggctcc tttcaggtgg acccgtgcca ttatcgccgg ttgatttggc
    34021 gtatcgcttt atgcgaaagg cgatgaaatt gttcggggcg cactctgcgg gcctgcatct
    34081 cggaatgagc accggctttg attcaggcag ttccctggat tatgtctatc aaaatcaacc
    34141 gcaaggcagt aacgcattcg ggcgcttaat tgacaaaatc tacctgaaca gtgttggctg
    34201 gcgcggtatt cgccagcgta aaacccattt acaaatactg attaaacaag ccgttgccga
    34261 tctccacgcc aaaggtttag ccgtccgcgt ggttgacatt gccgcagggc atgggcgcta
    34321 tgtactggat gcgctggcaa acgagcctgc cgtaagcgat attttgttac gtgattacag
    34381 tgagttaaat gttgcacagg ggcaagcgat gattgcccaa cggggaatgt ctgggcgggt
    34441 gcgttttgaa cagggcgatg cgtttaaccc ggaggaactc agcgcgttaa ctccgcggcc
    34501 tacgctggcg attgtctctg gcctgtatga gctttttccc gaaaatgagc aggtaaaaaa
    34561 ctcactcgca ggtcttgcca atgccatcga accgggcggc attctcatct acaccgggca
    34621 gccgtggcac cctcaactgg agatgattgc cggggtgtta accagtcata aagatggtaa
    34681 accgtgggta atgcgcgtgc gttcgcaagg ggagatggat tcactcgtgc gtgatgccgg
    34741 atttgataaa tgcacacaac ggattgatga gtggggtatt tttacggttt cgatggcggt
    34801 gcgtcgtgat aactgaacgt cggaacgtat tgctacaagg cgctggctgg ttattgttgc
    34861 tggccccgtt tttcttcttc acctatggat ctcttaatca gttcaccgcg gttcaggacc
    34921 ttaacagcca tgatatcccc agtcaggtat tcggttggga aacggcgatc ccttttcttc
    34981 cctggactat tgttccttac tggagtctgg atcttttata tggattttcg ctgttcgttt
    35041 gtagcacgac attcgaacag cgccgacttg tccaccggct tattctggca acggtaatgg
    35101 cctgctgcgg ttttttgctc tatccgctga agtttagttt tatccgtcct gaagtgagtg
    35161 gggtgacggg atggctattt tcgcaacttg aactgtttga tctgccttat aaccagtctc
    35221 cttcgctgca tattattctc tgctggctac tttggcgtca ctttcgtcag catctggctg
    35281 agaggtggcg taaagtctgc ggcggatggt ttttactcat cgctatttct actctgacga
    35341 cctggcagca tcattttatt gatgtcatca cagggctggc ggtaggtatg ttgattgact
    35401 ggatggtgcc cgtcgaccgt cgttggaatt atcagaaacc tgatcaacgt cgaatcaaaa
    35461 tagcactgcc ctatgtcgta ggcgcgggct cgtgcattgt attgatggag ctaatgatga
    35521 tgattcagtt atggtggtca gtctggttat gttggccagt attatcgcta ctcataattg
    35581 gccgtgggta cggtgggctt ggcgcgataa caacagggaa agatagtcag gggaaactac
    35641 cgcccgccgt ttactggctg acattgccct gccgcatcgg gatgtggctg tctatgcgtt
    35701 ggttttgtcg tcgcctggag ccggtgagca aaatgactgc tggtgtttat ttaggggcgt
    35761 ttccacgaca tattccggca cagaatgcgg ttctggacgt cacctttgaa ttccctcgcg
    35821 gacgagcgac aaaagatcga ctctatttct gtgtaccgat gctggatctg gtggttccgg
    35881 aagaggggga gctccgacag gccgtggcga tgctggaaac attacgcgaa gagcaaggca
    35941 gcgttctggt ccattgcgcg ttgggattat cgcgcagtgc gctggtagtg gcggcatggt
    36001 tgttatgtta cggacactgt aaaaccgttg atgaagcgat tagttatatt cgagccagac
    36061 gttcgcatat tgtgcttaag gaagatcaca aagcgatgct gaaattatgg gaaaacaggt
    36121 aagtggattg agatgtggac tgaactacag tccacatcaa gatcgtgtcc ggttatgcag
    36181 caacaatgct gtcgatggcc gctttcgcgt cagactgcgc tttcgctgcc atttccggac
    36241 cgtatgcgat cccttcggcg aagacaaatt tcacatcggt aatgccgata aagccgagga
    36301 acgtggacag atacggcgtc accaggtccg ttggtccatc tttgtggatc ccaccgcggc
    36361 tggtaataac gatggctttt ttacccgtta ccagaccttc cggaccgttc tcggtatagc
    36421 ggaaagtaac gcctgcgcgt gcaaccaggt caaaatagtt tttcaactga gttgagatgt
    36481 tgaagttata catcggtgcc gcaataacga taacgtcgtg ggctttcagc tcggcaatca
    36541 gctcatcgga aagcgccaga gcttcctgct gacgcggagt cagcggcgca tcgctcggac
    36601 gcagtgcacc aaccagttcg ccatccagta ccggaatcgg atttgcagcc aggtcgcgaa
    36661 cggtgatttc atcagcggag tgcttttcgc gccattgttc aacaaaataa tcggacaact
    36721 gattagactg agagtaccct gccaggatgc tggatttaag aactaatacc ttgctcatgg
    36781 tgtttcctta tagatgtttg aatgggcgat gccccgttgc ttgctgacac tttattcaca
    36841 atcctgccac agagatagcg caataaatcg aagcctatgt tcgaatttat tgaacaacgc
    36901 atagaaagcc gcgatgtggt actctatatc tatcatttaa aagaaaatta atcaggcaga
    36961 ctactgccca ctaacgttat gacagaacaa caaaaattga cctttacggc cttgcagcag
    37021 cggctggatt cgctgatgct gcgcgacaga ctgcgttttt ctcggcgtct gcacggtgtt
    37081 aagaaggtta aaaatcctga tgcacaacag gccattttcc aggagatggc gaaagagatt
    37141 gaccaggcgg cagggaaagt cctgctgcgt gaagcggcac gaccggaaat tacttatcct
    37201 gacaatttac cggttagtca gaaaaaacag gacattctcg aagcgattcg tgatcaccag
    37261 gtggtgatcg tcgccgggga aacgggttct ggtaaaacga ctcagttacc gaaaatctgt
    37321 atggagctgg ggcgcgggat taaagggctg atcggccata cccagccgcg tcgcctggcg
    37381 gcaagaacgg tggcgaaccg tattgcggaa gagctgaaaa cggagccggg cggttgcatc
    37441 ggttacaaag tgcgtttcag cgatcacgtt agtgataaca cgatggtcaa gctgatgacc
    37501 gacggtatcc tgctggcgga gatccagcaa gaccgcctgc tgatgcagta cgacaccatc
    37561 attattgacg aagcacacga acgcagcctg aatatcgatt ttctgctcgg ctatttgaaa
    37621 gagttgctgc cgcggcgtcc tgacctaaaa atcattatca cttccgcgac tatcgacccg
    37681 gaacgctttt cgcgccactt taataatgcg ccgattatcg aagtctccgg tcggacctat
    37741 ccggtggaag tgcgctatcg accgattgtt gaagaagccg acgacaccga gcgcgatcag
    37801 ttgcaggcga tttttgacgc cgtagacgaa ctgagtcagg aaagccctgg cgacattctg
    37861 atctttatga gtggtgagcg ggaaattcgc gataccgccg atgcgctgaa caagctaaat
    37921 ctgcgtcata ccgaaatctt gccgctttat gcgcggcttt cgaacagcga acagaaccgc
    37981 gtgttccagt cgcacagcgg acggcgcatt gtgctggcga ccaacgtcgc ggaaacctcg
    38041 ctgaccgtac cggggattaa atacgttatc gaccccggta cagcacgtat cagccgctac
    38101 agctatcgca ccaaagtgca gcgtttgccg attgaaccca tttcgcaggc gtctgccaat
    38161 cagcgtaaag gccgctgtgg gcgtgtgtcc gaagggatct gtattcgtct ctattccgaa
    38221 gacgatttcc tttcgcgccc ggagtttacc gatccggaga ttctgcgtac caacctggcc
    38281 tcggttattt tgcagatgac cgcgctgggg ctgggcgata tcgctgcgtt cccgtttgtc
    38341 gaagcaccgg ataaacgcaa tatccaggat ggcgtgcgtc tgctcgaaga actgggcgcg
    38401 atcaccacgg atgaacaggc cagcgcctat aaactgacgc cgctcggtcg ccagctctcg
    38461 cagttgcctg tcgatccacg tctggcgcgt atggtgctgg aagcgcaaaa acatggctgc
    38521 gtgcgtgagg cgatgattat cacgtccgcg ctttccattc aggatccgcg cgagcgtccg
    38581 atggataaac agcaggcatc ggacgaaaaa catcgtcgct tccacgacaa agagtctgac
    38641 ttcctcgcgt ttgtgaatct gtggaattat cttggcgagc agcaaaaggc gctttcttcc
    38701 aacgccttcc gtcgcctgtg tcgtaccgat tatctcaact atctgcgcgt gcgcgaatgg
    38761 caggatatct acacccagtt gcgccaggtg gtgaaagaac ttggcattcc ggttaacagc
    38821 gaaccggcgg agtatcgcga aattcacatt gcgttgctga cgggtttact ttcccatatc
    38881 ggcatgaaag atgccgataa acaagaatat accggcgcac gtaacgcgcg tttctccatc
    38941 tttcccggtt ctggtttatt caaaaaaccg ccgaaatggg taatggtggc ggaactggta
    39001 gaaaccagcc gcctgtgggg gcgcattgct gcgcgtatcg acccggaatg ggtggagcct
    39061 gtcgctcagc atttgattaa acgcacctac agcgaacctc actgggaacg ggcgcagggc
    39121 gcggtgatgg caacggaaaa agtcactgtt tatggtttgc cgattgttgc cgcgcgcaag
    39181 gtcaactaca gccagatcga tccggcgtta tgccgtgaac tctttattcg ccatgcgctg
    39241 gtggaaggtg actggcagac gcgtcacgca ttcttccgtg aaaacctgaa actgcgggcc
    39301 gaagtggaag agctggaaca caaatcacgt cgccgcgata ttctggttga tgacgaaacg
    39361 ttgtttgagt tctatgacca gcgcatcagc cacgatgtga tctctgcacg ccacttcgac
    39421 agctggtgga aaaaagtcag ccgcgaaacc cctgatttgc tcaactttga aaaaagcatg
    39481 ttgatcaaag agggcgcaga aaaaatcagc aagctggatt acccgaactt ctggcatcag
    39541 ggcaatctca agctgcgttt gagctatcag tttgagcccg gcgcggatgc tgacggtgtg
    39601 accgtacata ttccgctgcc gttacttaac caggttgagg aaagcgggtt tgaatggcag
    39661 atccccggtc tgcgccgcga actggtgatt gctctgatta aatcgttgcc gaaaccggta
    39721 cgccgtaatt ttgtacccgc accaaactac gccgaagcgt ttttaggccg cgtcacaccg
    39781 ctggagttac cgttgctcga cagccttgag cgcgagttac ggcggatgac cggtgttacc
    39841 gttgaccgcg aagactggca ctgggatcag gtgcccgatc acctgaaaat caccttccgc
    39901 gtggtggacg acaaaaataa gaagctaaaa gaagggcgct cattacagga tctgaaagat
    39961 gcgctgaaag gcaaagtgca ggaaacgctg tccgcggtgg cggatgacgg tatcgagcag
    40021 agcgggctgc atatctggag ctttgggcaa ctgccggaaa gctacgaaca gaagcgcggc
    40081 aactataaag tgaaggcgtg gccggcgctg gtggatgaac gtgacagtgt ggcgatcaaa
    40141 ctgtttgata acccgctgga gcaaaaacag gcaatgtgga acggtcttcg ccgtctactg
    40201 ctgctaaata ttccctcgcc gatcaagtat ttacatgaga agttaccaaa caaagccaag
    40261 cttggactgt actttaaccc gtatggcaaa gtgctggagc tgatcgacga ctgtatctcc
    40321 tgcggtgtgg atcaactgat cgacgccaat ggtggcccgg tctggacgga agaaggcttt
    40381 gctgcgctgc atgagaaagt gcgtgccgaa ctgaacgaca cggtggtaga tattgcgaag
    40441 caggtcgagc aaattcttac ggcagtgttc aatatcaaca aacgtctgaa agggcgggtg
    40501 gatatgacca tggcactggg gctttctgac attaaagcgc agatgggcgg gttggtatat
    40561 cgcggttttg tcactggtaa cggcttcaaa cggctgggcg acacgctgcg atatttgcag
    40621 gcgattgaaa aacggctgga aaaactggcg gttgatccac atcgcgaccg tgctcagatg
    40681 ctgaaagttg aaaacgtcca gcaggcgtgg cagcaatgga tcaacaaact gccgcctgca
    40741 cgtcgtgagg atgaagacgt gaaagagatc cgttggatga tagaagagtt gcgcgttagt
    40801 tacttcgctc aacaacttgg tacgccttat ccgatttcag ataagcgtat tttgcaggcg
    40861 atggagcaga ttagcggtta accctgctat ttgcctgata aagaaaaaac ccggtaagca
    40921 tttagcgccg ggttttttat taattctaaa acggtaaggg taaaaattca ggaattcaga
    40981 aaaatacaat tctctgctgc aagatgaata atgtttatct acagcatttc cttaaaagat
    41041 atgtcaggct tgcggagtgg cggttaagga catacgactt cctcctttca gagtgctccg
    41101 cttctcacta ttatctcaca cagtattctt aagggaacga taaggaggaa ccatgaacat
    41161 taccccgttt ccgacgcttt cgccggcaac tatagatgcc ataaatgtta tcggacagtg
    41221 gctggcgcag gatgatttct ccggtgaggt gccgtatcag gccgattgcg tgatccttgc
    41281 aggcaatgcg gttatgccga ctatcgacgc ggcatgtaag attgctcgcg atcagcaaat
    41341 tcctttactg attagtggtg gtatcggtca ctcgacaact tttttgtata gcgccatcgc
    41401 acagcatccg cactacaaca ctatccgcac cactggcaga gcagaagcga ccatcctggc
    41461 ggatatcgct catcagttct ggcacattcc gcatgaaaaa atctggattg aagaccagtc
    41521 aacaaactgc ggtgaaaacg cacgctttag catcgcgcta ttgaaacagg ccgtagaacg
    41581 agttcatacg gctatcgttg ttcaggaccc caccatgcag cggcgcacga tggcgacgtt
    41641 ccgccgtatg actggggaca atcccgatgc accacgctgg ttaagttatc ccggattcgt
    41701 tcctcagtta ggaaataacg cagacagtgt aatctttatt aatcagttac aaggattatg
    41761 gccggttgag cgttatctct cactactcac tggcgagctg ccgcgtttac gcgatgatag
    41821 cgatggctac ggtccccgcg ggcgagattt tatcgttcac gttgattttc cggcagaagt
    41881 catccatgca tggcaaacgc tgaaacatga tgcggtgctc atcgaggcga tggaaagtcg
    41941 ctcgttacgt taaaaattgc ccgtttgtga accacttgtt tgcaaacggg catgactcct
    42001 gacttttatt tctgcctttt attcctttta cacttgtttt tatgaagccc ttcacagaat
    42061 tgtcctttca cgatgccgtc tctctgatga ttgatgttaa ttaacaatgc attcaccgaa
    42121 aataaacata taaatcacag gagtcgccca tgtcagtacc cgttcaacat cctatgtata
    42181 tcgatggaca gtttgttacc tggcgtggag acgcatggat tgatgtggta aaccctgcta
    42241 cagaggctgt catttcccgc atacccgatg gtcaggccga ggatgcccgt aaggcaatcg
    42301 atgcagcaga acgtgcacaa ccagaatggg aagcgttgcc tgctattgaa cgcgccagtt
    42361 ggttgcgcaa aatctccgcc gggatccgcg aacgcgccag tgaaatcagt gcgctgattg
    42421 ttgaagaagg gggcaagatc cagcagctgg ctgaagtcga agtggctttt actgccgact
    42481 atatcgatta catggcggag tgggcacggc gttacgaggg cgagattatt caaagcgatc
    42541 gtccaggaga aaatattctt ttgtttaaac gtgcgcttgg tgtgactacc ggcattctgc
    42601 cgtggaactt cccgttcttc ctcattgccc gcaaaatggc tcccgctctt ttgaccggta
    42661 ataccatcgt cattaaacct agtgaattta cgccaaacaa tgcgattgca ttcgccaaaa
    42721 tcgtcgatga aataggcctt ccgcgcggcg tgtttaacct tgtactgggg cgtggtgaaa
    42781 ccgttgggca agaactggcg ggtaacccaa aggtcgcaat ggtcagtatg acaggcagcg
    42841 tctctgcagg tgagaagatc atggcgactg cggcgaaaaa catcaccaaa gtgtgtctgg
    42901 aattgggggg taaagcacca gctatcgtaa tggacgatgc cgatcttgaa ctggcagtca
    42961 aagccatcgt tgattcacgc gtcattaata gtgggcaagt gtgtaactgt gcagaacgtg
    43021 tttatgtaca gaaaggcatt tatgatcagt tcgtcaatcg gctgggtgaa gcgatgcagg
    43081 cggttcaatt tggtaacccc gctgaacgca acgacattgc gatggggccg ttgattaacg
    43141 ccgcggcgct ggaaagggtt gagcaaaaag tggcgcgcgc agtagaagaa ggggcgagag
    43201 tggcgttggg tggcaaagcg gtagagggga aaggatatta ttatccgccg acattgctgc
    43261 tggatgttcg ccaggaaatg tcgattatgc atgaggaaac ctttggcccg gttctgccgg
    43321 tggtcgcatt tgacacgctg gaagaggcta tctcaatggc taatgacagt gattacggcc
    43381 tgacctcatc aatctatacc caaaatctga acgtcgcgat gaaagccatt aaagggctga
    43441 agtttggtga aacttacatc aaccgtgaaa acttcgaagc tatgcaaggc ttccacgccg
    43501 gatggcgtaa atccggtatt ggcggcgcag atggtaaaca tggcctgcat gaatatctgc
    43561 agacccaggt ggtttattta cagtcgtaat gagtaaaaga ggcggaggtt ttttcctccg
    43621 cctgtgcgcg tcagagttta gcgaattttt cgagggtgcg aataagctgc gtgacgaagc
    43681 catattcgtt atcgtaccag gcgaccgttt tcaccagttg taaatcgccc acggcggtaa
    43741 tttccgtttg cgtggcatca aacaccgaac cgaaatggct gccaatgata tcggaagaga
    43801 ctatttcttc atcggtataa ccaaatgact cgttattggt ggttgcttgt ttaagtgcgt
    43861 tattcacctc ttcggcagtc actttttttc cgagaatcga taccagttca gtgaccgaac
    43921 ctgttttcac cggcacgcgt tgcgcatgac ctttcagttt gccgctcagt tccgggatca
    43981 ccagaccaat ggcttttgcc gcccccgtag tgtggggaat gatattttct gccgctgcgc
    44041 gtgaagcacg taaatcttta ccacgtgggc catccaccag tgactgggtg ccagtatagg
    44101 catgaatggt cgtcatcgtg ccgacttcta tcccgaaact gtcatgcaag gctttggcca
    44161 tcggcgcaag acagttagtg gtgcatgacg ccacggaaac aatggtgtcg ttgccatcca
    44221 gagtgtcgtc attgacgtta taaacgatag ttttcatttc accggcaggg gcggaaatca
    44281 acaccttctt cgcaccagca tcaagatgcg cctgcgattt ctcggcggag gtataaaagc
    44341 cagtacattc gacaatgatt tctgcccctt tcgctttcca cggaatattt ttagcctctt
    44401 tttcggcgta aaccgcgata cttttcccat caacgataag tgaatcttcc gtaaaatcaa
    44461 cgctccaggg gaacggacca tagtttgaat catgtttcag cagataggcg agaatttttg
    44521 gggaagtgag atcattaata gcgacaacgt ctatgttgct tttgacttca agtaatcgac
    44581 gcaacaccag tcgaccgata cgaccaaaac cgttaatacc aactttactc atggttttct
    44641 cctgtcagga acgttcggat gaaaattgat cctttccagg cttagcccag gattgtggga
    44701 tgggcaatct ccattcttac agtgaaacgt aacgtactga aaacgggtga acaatattta
    44761 atgaaatttt gagaaaagcc cgttatgtta acggaaaatt atgttaaagc aggaaatgtt
    44821 atggaaaata aatactcaag gttacaaatc agcattcact ggctggtctt tttgctggtt
    44881 atcgcagcgt attgcgcaat ggagtttcgt ggtttcttcc cacgtagcga tcggccactc
    44941 atcaacatgg ttcatgtttc ctgtggtatc tcaattctcg tgctgatggt ggttcgtctt
    45001 ttgttaagac tgaaataccc aaccccgccg attataccta agccaaaacc gatgatgacg
    45061 ggactggcgc atttgggaca tttggtgatt tatctcctgt ttattgcgct gccagtgatt
    45121 ggtttggtga tgatgtataa ccggggcaac ccgtggtttg cgtttggttt gacgatgcct
    45181 tacgcttcag aggccaattt cgaacgggtt gatagcttaa agtcgtggca tgagacgctg
    45241 gcgaatctgg gatattttgt catcgggttg cacgctgcgg ctgcactggc acaccactat
    45301 ttctggaagg acaacacact tctacgcatg atgccgcgta aacgttcctg aaggatattt
    45361 aaagaaaacg cctgtactaa aaccgacccg tggtacaggc gaagaatacg ggtctacatc
    45421 ggaagcgcct attatattta tttgtatgac aaataaaacc ataatccttg cccatacgtc
    45481 catctggctt atttttaatc aattcacccg atctttgatc tcatcaacgg tatcaaaata
    45541 aaccagcgta taacgttcaa atcgaacata agattcgata aaccatggag gttatatgaa
    45601 aaaactggca cttattttgt ttatgggaac gcttgtttcc ttttatgccg atgccgggcg
    45661 caaaccctgt tctggttcga aaggggggat ctcacactgt acggcaggcg gcaaatttgt
    45721 ctgtaatgat ggttctatta gtgcctcgaa aaaaacatgc actaactgaa gtgtaaaagg
    45781 ggtgccatga gaaaatgaat ctgctagtca gatgcgcggg gaaaatcccc gcgcttgccc
    45841 ttacctggac gtgcaggcca tgagcgcagc aacctcctta tcaccgtccc ggaaccgcag
    45901 ttcgtagagc gtttgtcggg tcaggagtgt gaatgtcaga atcgtaatgc agataatgag
    45961 cagacacacc accagagggt tgtgcttcat agcctttctc cttgccggat ggcgggtaag
    46021 aggctaagat ctgaattgct aggttcattc gttggcctcg gttgatagaa atatcggtcg
    46081 gggccttcgt ctttctgatt cccggttagc ctgaaaacag aaagtctcag gcacccgcag
    46141 gcatcctatg aggtttcctt agggacgaaa ataatcactt cacgaaattg cgtgctgttt
    46201 tccagaattt ttcgtcattc gggttagcca gtttagccat tcgttactct ctttattcca
    46261 atagcattaa ttttctctgc aataattgtt gtaaaaatgt gacgcaaaga ggtttttggt
    46321 cataagtaat taccgtcaag tgccgatgac tttctatcag gagtaaacct ggacgagaga
    46381 caacggtaat gaatacaact ccctcacatc gattaggttt tttgcatcac atcaggctgg
    46441 ttccgttatt tgcctgcatt cttggcggta tcttagttct attcgcatta agttctgctc
    46501 tggctggcta tttcctctgg caggccgatc gcgatcagcg tgatgttact gcggagattg
    46561 agattcggac cgggttagcg aacagttcag attttttgcg ttcagcccgg atcaatatga
    46621 ttcaggccgg ggctgcgagt cgtattgcgg aaatggaagc aatgaagcgc aatattgcgc
    46681 aagccgaatc ggagattaaa cagtcgcagc aaggttatcg tgcttatcag aatcgaccgg
    46741 tgaaaacacc tgctgatgaa gccctcgaca ctgaattaaa tcaacgcttt caggcttata
    46801 tcacgggtat gcaacctatg ttgaaatatg ccaaaaatgg catgtttgaa gcgattatta
    46861 atcatgaaag tgagcagatc cgaccgctgg ataatgctta taccgatatt ttgaacaaag
    46921 ccgttaagat acgtagcacc agggccaacc aactggcgga actggcccat cagcgcaccc
    46981 gcctgggtgg gatgttcatg attggcgcgt ttgtgcttgc cctggtcatg acgctgataa
    47041 catttatggt gctacgtcgg atcgtcattc gtccactgca acatgccgca caacggattg
    47101 aaaaaatcgc tagtggcgat ctgacgatga aggatgaacc ggcgggtcgt aatgaaatcg
    47161 gtcgcttaaa tcgtcattta cagcaaatgc agcattcact ggggatgaca gtagggactg
    47221 ttcgacaggg tgcggaagag atttatcgtg gcaccagcga aatttcagct ggcaatgcgg
    47281 acctgtcatc tcgcaccgaa gaacaagcgg cggcaatcga acaaactgcc gccagcatgg
    47341 agcaactcac tgcgacggtg aaacagaatg cggataacgc gcatcatgcc agcaaactgg
    47401 cgcaagaggc ttctattaaa gccagcgatg gcgggcagac ggtttccggt gtagtaaaaa
    47461 cgatgggcgc tatctccacg agttcgaaga aaatttctga gatcaccgcc gtcatcaaca
    47521 gtattgcttt ccagacgaat attctggcac tgaatgctgc cgttgaagcc gcgcgagcgg
    47581 gtgagcaagg gcgtggattt gccgttgtcg ccagcgaagt acggacactc gcaagccgca
    47641 gcgctcaggc ggcgaaagag attgaaggct tgatcagtga atcagtcagg ttaattgacc
    47701 tggggtcgga tgaggtggca acggcaggga aaaccatgag cactattgtt gatgccgtcg
    47761 cgagtgtcac acatatcatg caggaaatcg ccgccgcctc ggatgaacaa agtagaggca
    47821 taactcaggt tagccaggcg atttctgaaa tggataaggt gacgcaacag aatgcttctc
    47881 tggtagaaga ggcctcagca gcggcggtgt cccttgaaga acaggctgca cgattaactg
    47941 aggcggtgga tgtattccgt ctgcacaaac attctgtgtc ggcagaacct cgcggagcgg
    48001 gtgaaccagt tagtttcgct acggtgtgaa aatgttcaag gagggatcga cagatccctt
    48061 cacctttcag aacggcattg attttcgaat agcgttaatc atcaactggc aaccagaaga
    48121 gaacgtcgca tctacgcggg tcagtattcc aatcggttcg cctgcaccat gtcccggaac
    48181 aggcagggcc accagcgtgg catgacgcag gtcgtctttt acagcgccag aagggacaaa
    48241 ccacacgtaa tcgtattcaa ccgtaagttg acgagatagc gaagcagaca gcgtttcgat
    48301 acaacccgaa ggaattttac agccctggct ctgcactaat gcatctgaat gctggcgtgg
    48361 cgcagtgcct tctggtgata caacgaccgg ccattccagc acccggctta gcgttacgtt
    48421 ctcctgaagt agcgggtgat tagggcggac aaccagcttc aacgattcaa gaaacagcag
    48481 ttcgtaatta agcccggtca tcagttcagg atctgacatc cgaccaatgc cgatatcgat
    48541 ttccccggtt tttaaacccg ccagaatcat agggttactc attgtcgcaa cttgcaaggt
    48601 cgtctctttt tgttgctgat gaaactgacc tataaccgaa ggtaatatcc ccagtgccgc
    48661 agtaggtagt gcaccaaccc tgacgacatc attattaaga ccttctttac gatgaagcga
    48721 ctgtccggca gtgttgatgg cgtcaagaac tctgactgca tgcgttaaaa attgttcgcc
    48781 gggtaaggta agttgcgccc cctgacgacc acgctcaaac aagcgagcgc cagtcagctg
    48841 ctccagttca ttcaatgtct tagagagcgc aggttgactc aaattaaggg tttcagccgc
    48901 gcgccccaaa gttccttgtt gtgcgacagc tacgaatgta tgaaggtggc gcaaacggat
    48961 gcgctgacta aacagactat ttttttccat aagcgatgtt aaaaacgaag cggtgtcgct
    49021 gacaagtgaa gttgtttgat tatgataact tgattgcaaa atattattaa caattaaagc
    49081 aattatgtta cagcaaaatg gataatattg atgttttcgc ggcgagatca cagtttgtaa
    49141 attcttcccg caagagtgaa tgcggttacc tacactccag attactgacc actggaggca
    49201 gacactatgg cgaacagcat cacggcggat gagattcggg aacagttttc gcaggcaatg
    49261 tcagccatgt accagcaaga agttccgcaa tatggcacgc tgctggaact ggtagctgat
    49321 gtgaatctgg ctgtgctgga aaacaatcct caactgcacg aaaaaatggt aaatgcagac
    49381 gagctggcgc gactgaatgt tgaacgtcat ggggcgattc gcgttgggac tgcacaagag
    49441 cttgctactc ttcggcggat gtttgccatt atggggatgt acccggtgag ctattacgat
    49501 ctctcgcagg caggggtgcc ggtacattcg acagcatttc ggcccattga tgatgcttct
    49561 ctggcgcgta atcccttccg cgtttttacc tccttactcc gccttgagct tatcgagaac
    49621 gaaattttgc gccagaaagc ggcggagatt ctgcgtcagc gcgatatctt caccccacgt
    49681 tgtcgacaac tgttagagga atatgagcag cagggcggtt ttaacgaaac acaggcacag
    49741 gagtttgtgc aggaagccct ggaaacgttt cgctggcacc agtcagcaac ggtagatgaa
    49801 gaaacctatc gcgccttgca caacgaacat cggttgattg ctgatgtggt ctgttttcct
    49861 ggatgccata tcaaccacct gacaccacgt acgctggata ttgaccgggt gcagtcgatg
    49921 atgcctgaat gcggaattga acccaaaatt ctcatcgagg ggccgccgcg ccgcgaggta
    49981 ccgattttac tacgccagac cagctttaaa gcactggaag agacggtgtt gtttgcgggg
    50041 cagaaacagg gcacgcatac cgcgcgcttt ggtgaaattg agcagcgtgg cgtggcatta
    50101 acgccgaaag ggcgacaact gtatgatgat cttctgcgta acgctggaac cgggcaggat
    50161 aatctcactc accaaatgca tttacaggaa accttccgca cttttcctga cagtgagttt
    50221 ttaatgcgtc agcaagggct ggcatggttc cggtaccgtc tgacgccttc aggtgaggcg
    50281 catcgtcagg cgattcatcc cggagacgat ccacagccct taattgaacg tggttgggtc
    50341 gcggcgcaac ccattaccta tgaagatttc ttgcccgtta gcgcggcggg gatcttccag
    50401 tcaaatctgg gtaatgaaac gcagacacgc agtcacggta atgccagtcg cgaagcattt
    50461 gagcaggcgt tgggttgtcc ggttttggat gagttccagc tttaccagga agcggaagaa
    50521 cgcagtaaac gtcgctgtgg tttgctttaa attctgacca tccgcctttg caaaaatttg
    50581 cctgatttta caaacgaatc aggctcatcc catcgacata aaaaaaatgc cgatttatgc
    50641 atattctctc agttcaacaa ttggattatt aataaatatt gtctagagtg agcggtcata
    50701 aataagcact ttcttgccgc tgaaaacgac cagcgcggga ccattcacaa caccagaagg
    50761 actcactttc aggtatggat cgtagacgat ttattaaagg ttcaatggct atggccgccg
    50821 tgtgcggtac cagcggcatt gcttctcttt tttctcaggc ggcattcgcg gcagattctg
    50881 atattgccga cgggcaaacc cagcgttttg acttctccat tctacagtca atggcgcacg
    50941 acttagcgca aacagcgtgg cgtggtgcgc ctcgtccgtt acctgacacg ctggcgacaa
    51001 tgacgccgca ggcttataac agtattcaat acgacgccga aaaatcgctc tggcataacg
    51061 ttgagaaccg tcaactggac gctcagttct tccatatggg aatgggattc cgtcgccgcg
    51121 ttcgtatgtt ttctgtagat ccagcaacac atctggcgcg tgaaattcac tttcgcccgg
    51181 agttgttcaa atacaacgat gcgggtgttg atacaaaaca attagaaggg caaagcgatc
    51241 tcggctttgc cggttttcgc gtgtttaaag cccccgaact ggcgcgccgt gatgtagtat
    51301 catttctcgg cgcgagttat ttccgcgccg ttgatgatac atatcaatac ggtttgtcgg
    51361 cccgcggcct ggcgatcgac acttacactg acagtaaaga agagttcccc gactttaccg
    51421 ccttctggtt tgatacggta aaaccggggg caactacctt taccgtttat gcgttgctcg
    51481 atagcgccag cattactggt gcctataagt tcactatcca ttgtgagaaa agtcaggtga
    51541 ttatggatgt ggaaaatcac ctgtatgcgc gcaaagacat taaacagctg ggcattgcgc
    51601 cgatgaccag tatgttcagc tgcggtacta atgaacgtcg gatgtgcgat acaattcatc
    51661 cgcaaattca tgactctgat cgtctgtcca tgtggcgggg caacggcgag tggatttgcc
    51721 gtccgctgaa taatccgcaa aaattgcagt tcaatgctta caccgacaac aacccgaaag
    51781 ggtttggttt attgcaactg gatcgtgact tctcccatta tcaggacatt atgggctggt
    51841 ataacaaacg cccaagtctg tgggtggagc cgcgtaacaa gtggggtaag ggcaccatcg
    51901 gcctgatgga aatcccaaca acgggcgaaa cgctggataa cattgtctgc ttctggcagc
    51961 cagaaaaagc tgtaaaagca ggtgatgagt ttgcattcca gtatcgtctg tactggagtg
    52021 cgcaaccgcc tgttcattgc ccattagcgc gcgttatggc gacgcgtacc ggcatgggcg
    52081 gtttcccgga aggttgggca ccaggtgaac actatcccga aaaatgggcg cgtcgttttg
    52141 ccgtcgattt cgttggtggt gatctgaaag ctgccgcacc aaaaggcatt gagccggtga
    52201 ttacgctttc cagtggggaa gcgaagcaaa tcgaaattct ctatattgaa cccattgatg
    52261 gttatcgtat tcagtttgac tggtatccga cttcggactc cactgatccg gtcgatatgc
    52321 ggatgtatct gcgttgtcag ggcgacgcta tcagtgaaac atggctgtat cagtatttcc
    52381 cgccagcgcc ggataaacgt cagtatgttg acgaccgcgt gatgagttaa tcgttttttc
    52441 ttcggcacct tcttcgggag gtgccgtctg gttaaacacg atcccgctcg catttttccc
    52501 taagttaaat gagtaatctg atggtgtgta tttcagatac accttgtcag ccactaacag
    52561 ggagtgcgta tgtttccaga ataccgagat ttaatatccc gtctgaaaaa cgaaaatcct
    52621 cgctttatgt ccttgttcga taaacacaat aaacttgatc atgaaattgc cagaaaggaa
    52681 ggttccgacg gtcgagggta caatgcggaa gtggtccgca tgaaaaaaca aaagctacag
    52741 ttaaaagatg agatgctcaa aatcctgcag caggagagcg tcaaagaggt gtaaactttc
    52801 ctaagccgcc agccaggcgg cttttttaac aacagcatgg attgactgga gataagatga
    52861 ctgaaacgat aaaagtaagc gaatcacttg aattacatgc tgttgcggaa agtcacgtca
    52921 cacctcttta tcagttaatc tgtaaaaata aaacctggtt acagcagtcg ctaaactggc
    52981 cgcagttcgt tcagagtgaa gaggacacgc gaaaaacggt gcagggtaat gtgatgttgc
    53041 atcaacgcgg ctatgccaaa atgttcatga ttttcaaaga agatgaactt atcggcgtta
    53101 tctcgtttaa tcgtattgaa ccactgaata aaactgctga aatcggctac tggctggacg
    53161 aatctcatca ggggcagggg atcatttctc aggcgctgca ggcattgatt catcattacg
    53221 ctcagtctgg tgaacttaga cgcttcgtga tcaaatgtcg ggtggacaat ccgcaaagca
    53281 accaggtcgc tttgcgcaat ggttttatcc ttgaaggttg cctgaaacag gctgagttcc
    53341 tgaatgatgc ctatgatgat gtgaacttat acgcgcgtat tatcgattca taataaccct
    53401 gccagcggcg ttcgcgtaat gcgctcctcg ccgttaatga cttttggacc acggacatga
    53461 accgtgtcgc catcaaaggc ttcaacgacg gcgtggtcgg ttaactccac atggttttca
    53521 attatcaccg cacctgaaat acggctctca ccttgtatta caacgtgctc gtcgagcaga
    53581 atcggtccac cacgtaccac ggcatttccg ccaatcaaga catgatgttt taacacacaa
    53641 ttaccttcca caatggcata ttccgccacc tgcgaactgt aatgaatcgt cggaatggca
    53701 tcttcttcta tgccagcttt cacctgcgcg tgaccgtaga ctttagcgca atcgcatagc
    53761 cagacgttgt tctcttcatt accttcaaca ctggcaaaat caaaaacttc ggcgcgatgt
    53821 tcaataaaag cataccggac gacggcatcg ccataaattt gtgcctggtg gacaatacgc
    53881 gaggcgctaa cccttgcgcg atcataaatt tggagcagga gttgatggtc gggcgttaag
    53941 ccttgtgcgg cgacgatcat agaatgttga tcaattaagg cgtgaccaaa tattctacat
    54001 tgcccgtaaa ctaatgagtc atgaatggtg acactgtcac ttatgtatgc accctggctg
    54061 atttcactat tatcgatcca gacattatcc gttgcgtaaa cctctcccca cagtacgctg
    54121 gtgccggtga tgcgcgtatt accggaaatc accgttccgc cgaacgcaat agcattctgg
    54181 tcgtaaatcc agcaatttcc ttcctgtgct aacaccgttt cgcgatcgat ccagccgcca
    54241 gcggttcctg ctatcacatc gttaaaatcg ctaatggcga taatctgccg taataacaca
    54301 ttttttttag tgccatcttc ctgataacta aaggctcgct gttcttcact tagacgatat
    54361 ttgcgcatag cgttttccca caggtgactt actataaccg tagcaaattc tgcggctctg
    54421 gctatgctcg agaaaattcc ataaaatgca tttcaaatat actttataaa ttaaacaaaa
    54481 tgagtaagaa gatgcagagc gataaagtgc tcaatttgcc ggcaggctac tttggtattg
    54541 tgttggggac gatagggatg ggatttgcct ggcgctatgc cagccaggtt tggcaggtca
    54601 gccactggtt aggggatggg ctggtgattc tggcgatgat catctgggga ttattaacta
    54661 gcgcatttat tacccgactc atacgctttc cgcatagcgt gctggcggaa gttcgccatc
    54721 cagtgctgag cagttttgtg agtttgtttc cggcaacgac gatgctggtg gcgattggtt
    54781 ttgttccgtg gtttcgccca ctggcggtgt gcctgttcag ttttggtgtc gtggttcagt
    54841 tggcttatgc cgcctggcaa actgcgggat tatggcgcgg atctcaccct gaagaagcta
    54901 ccacgcctgg actgtatctg ccgacagttg ccaacaactt tatcagcgca atggcctgtg
    54961 gtgcgttggg ctacaccgac gccggtctgg tgtttttagg cgcaggcgtt ttctcatggc
    55021 taagccttga accggtgatc ttgcagcgtc tgcgcagttc gggagaatta cccacggcac
    55081 tgcggacatc actcggcatt cagctcgctc ctgcgctggt ggcttgtagt gcctggctga
    55141 gcgtcaacgg cggcgagggt gacacgctgg cgaaaatgct tttcggttat ggactgctgc
    55201 aactgctgtt tatgctacgt ctgatgccat ggtatctctc ccagccattt aatgcttcat
    55261 tctggagttt ctcgttcggc gtatctgcac tggcaaccac cggtttgcat ctggggagtg
    55321 gcagcgataa tggatttttc catacgctgg cggtgccgct gtttatcttt accaatttta
    55381 ttattgcaat actgctcatc cgtacttttg cgcttctgat gcagggaaaa ttgttagtca
    55441 gaaccgagcg cgccgtttta atgaaagcag aggacaaaga atgatcattc gtgacgaaaa
    55501 ctattttact gataaatatg aattaacccg cacacactct gaagtactgg aagcggtgaa
    55561 agtggttaaa ccgggtaaaa cgctggatct gggctgtggc aatggtcgta acagtcttta
    55621 cctggcagcc aatggttatg atgttgacgc atgggataaa aatgccatga gtatcgccaa
    55681 cgtcgagcgc attaaatcca ttgaaaatct ggataattta cacacccgag tcgttgatct
    55741 gaataacctc acatttgata gacagtacga ttttattctt tcgactgtgg tactgatgtt
    55801 ccttgaggct aaaaccatcc cagggctgat tgtcaatatg caacgttgca ctaaacctgg
    55861 tggttacaac ctgattgtgg cggcgatgga taccgctgat tatccatgta ccgtcggctt
    55921 cccgtttgcc ttcaaagagg gagaattacg tcgatattac gaaggctggg agagggtgaa
    55981 atacaatgaa gacgtcggcg aactgcaccg caccgacgcc aacggtaatc gtattaaact
    56041 gcgtttcgcc acgatgctgg cacgtaaaaa atgacccgct aagaataagg cgcgtgaaaa
    56101 ttccccacgc tgagatgatt tactgttctt cttttcggta agcatatttt ttatcgaagg
    56161 gatgtgaaat taatcacagt agtcgaagtt ttttagcagc ttaacttact gaaatttaac
    56221 tactgatgat tgacttagcc cctttttcgg cattgactat gtcgtctgaa aaggggctga
    56281 aaaatttatt ttcaccaaca ctttttttgc cacaacacga agcggcgctt tttgctataa
    56341 cttagaaagt aatataatca tctcaggaaa ctattcatgc gtaccacatc atttgcgaaa
    56401 gttgcagctt tatgcggctt gttggctctg tctggttgtg cctctaaaat cacccagcca
    56461 gataaatatt ctggtttttt aaacaattac tctgatttaa aagaaacaac ctcagcaaca
    56521 ggtaaacctg ttttacgttg ggtagacccg agttttgatc aaagcaaata tgacagcatc
    56581 gtctggaacc caatcactta ttatccggta ccaaaacctt ctacccaggt agggcagaaa
    56641 gttctggata aaattttgaa ctataccaac accgaaatga aagaagcgat agcgcagcgt
    56701 aaaccactgg ttaccaccgc tgggccgcgt agtctgattt tccgtggggc cattaccggt
    56761 gtagatacca gcaaagaagg gctgcaattc tatgaagtgg ttcctgttgc attagtggtt
    56821 gcggggacac aaatggctac aggccaccgt accatggata ctcgcctcta ttttgaaggt
    56881 gagctgattg atgcagcgac taataaaccg gttatcaaag tcgttcgtca gggcgaaggt
    56941 aaagacctga ataacgaaag tacgccaatg gctttcgaaa atattaaaca agttattgat
    57001 gacatggcga ccgatgccac catgtttgac gttaacaaaa agtagttcca gacgcgccat
    57061 cgttagatgg cgctttttat ccggtgcgcc gtaaaacccc atccttcagg gcggggatat
    57121 aaggcgcggt tttccacctg accaggtgtt tgctgttgtt caatgtattg gcggatgacg
    57181 gatattggcg cacctccgca gctactggca aaatagccag gactccacaa aacgcctttg
    57241 tagtaatacc tgactgcaat atctggtcga tctcgtcgca gtaacctact cgatacgcct
    57301 ttgaggctgt ttaccagact ggatatcgcc agtttgggag gatagttaat taacaaatgg
    57361 acgtgatctg gttcgccatc catttaaacc agttcagctt caaaatcagc acatacattt
    57421 gaaaagtaag tgcgtagttt ttctgtcgcg tcatagtcaa aaatctgacg tcggtatctg
    57481 gtgacaaaga ccaggtgaac atgcatcagg aaaacacaat gcttccccgt cggatatcgg
    57541 tttctttatt catagaccaa agtatcattt ggcctgtgaa acgattacag gcatttaaat
    57601 tccagttaag acccggtggt caacaggagc gtgaaatgag gcgcttcgca ggcgcatgtc
    57661 gtttcgtttt caatcgtgct ctggcgcttc agaatgagaa tcatgaggcc ggtaataaat
    57721 acatccctta cgggaaaatg gcttcctggc tggttgagtg gaaaaacgcc actgaaacgc
    57781 aatggcttaa agattcgccc tcacagccat tgcaacagtc actgaaaggc cttgagcggg
    57841 cttacaaaaa cttcttccag aagcgggctg cttttccccg attcaaaaag cggggacaga
    57901 atgatgcatt ccgctacccg cagggcgtta agctcgatca ggaaaacagc cgtatttttc
    57961 tgccgaaact gggctggatg cgctaccgga acagccgtca ggtcacggga gttgtgaaaa
    58021 atgtcactgt cagccagtcc tgcggtaagt ggtacatcag tattcagaca gaaagtgaag
    58081 tatcaactcc ggttcaccct tcagcatcaa tggtcgggct ggatgctggc gtggctaaac
    58141 tcgccacgct gtcagatggc acagtctttg agcctgtaaa cagttttcag aaaaaccaga
    58201 agacgccggc gagacttcag cgacagttaa gccgcaaggt caaattcagc aacaactggc
    58261 agaagcagaa acgcaaaata cagcgactgc attcctgtat cgcaaatatc cgcagggact
    58321 accttcacaa agtcacaacg accgtcagca aaaaccacgc aatgatagtc attgaggatt
    58381 tgaaggtcag caacatgtca aagtcagcag cgggtacggt cagccagccg gggcgcaatg
    58441 tccgggcaaa atcaggttta aaccgttcga tactggatca gggctggtat gaaatgcgcc
    58501 gccagcttga atacaagcag ctctggagtg gcggtcaggt gcttgctgtt ccgccagcgt
    58561 atacaagcca gcgttgcgcg tgctgtggtc atacagcgaa agaaaatcgt ctgtcacaaa
    58621 gtaaattcag atgccaggta tgtggatata cagcgaacgc cgatgtaaac ggcgctcgta
    58681 acattttagc ggcggggcac gccgttcttg cctgtggagg gatggtgcag tcaggccgcc
    58741 cgttgaagca ggaacccacc gaaatgattc aggcgacagc ctgaacgtag cagggatcca
    58801 cgtccttcag ggcgtggagg atgtcaatat cggtttctgt cagcgattaa attcaacacc
    58861 acgtaacaaa cgcctccggc aattaatccc cagaacgcag aaccaatccc gaccagcgtc
    58921 aatccacttg ccgttaccag aaatgccacc accgccgcgt cgcgctcacg ctcattatgc
    58981 agcgcctgat acaaactgcc gccaatggta ctcaacagcg ccagacctgc cagcatctgg
    59041 atccaactta cgggcagggc agccatcatc ccggtaatgg cactaccaaa cagacctgcg
    59101 agcaaataga aaatgcctgc accggcagct gccagccaac gttgatcttt atccggatgc
    59161 gcttccgggc tttggcaaat agccgcggtg attgccgcaa taccgacgga ataaacgccg
    59221 aaaggggaaa aaaccagtgc cagcaatcca gtaaatacaa ttaatggcga aacaggagtt
    59281 gaatatccgg ctgctttcat tgctgcgata cccggtgcgt tttgcgatgc catcgtcacc
    59341 agaaaaaggg ggagtgcaac gctcaggctg tgagcaaacg aaaaatcagg ggtaatataa
    59401 gtggggagaa cgggtttaaa gacaacatca gttgtgacaa cgtcaccttg cgcgataacg
    59461 atcacgatcc caataatcat cgcggcaatt accgcatagc gcggcgcaac ggccttggtt
    59521 gccagccata ccagcaacat acttccacac aacgtaaatt gaccgtccag actggcaaac
    59581 gcctgtaaac caaagcgtaa taaaatcccg gcaagcattg ccgccgcaag cgagtgcgga
    59641 ataatgcgca tcagacgagc aaagagtccc gttatgccgc agaggactat taacgcgttg
    59701 gtgaaaataa aaacgccgat ggcttcgtta agtgttagtc cctgcaatcc ggtgaccaac
    59761 aaagccgcgc caggcgttga ccatgcggtg agaacaggta cgcgatacca taatgtcaga
    59821 gtcagcgtac tgacgcccat tgccagcccc agcgccgtca tccagccaga gatttgtgca
    59881 gtggtggctc cggcgacaat cgctgcttgc cagattattg ccgctgaact ggcgtagcca
    59941 attaataccg ccagaaaccc cgccagtagc gtgggtggag gaacagaaaa cagacgcata
    60001 gtcacctcgt gcgttatagc gtacaggcac cgtagcattt gtccgttata acgcacaagt
    60061 gataaacttc cgttttgccg gaggagtcgc atggaaaatc tcgctcgctt tttatccacc
    60121 acacttaaac aactacgcca gcagcgcggc tggagtcttt cgcgactggc agaagcgacg
    60181 ggcgtttcta aagcaatgtt agggcagatt gagcgtaatg agtccagtcc gacggtcgcg
    60241 acattatgga aaattgccac cgggttgaac gtaccatttt caacatttat ttctccgccg
    60301 cagcccgcta cgccttcagt ttatgatcca caacagcacg cgatggtgat cacttcgctg
    60361 tttccttatg atccacaact ctgctttgaa cacttctcga ttcagatggc acccggtgca
    60421 ataagcgaat cgacgcccca tgagaagggg gtaatcgaac atgtcgtcgt cattgatgga
    60481 caacttgatc tgtgcgttga tggggaatgg cagaccctta attgtgggga aggcgttcga
    60541 tttgctgcag acgtcacgca tatctatcgt aatggtgggg agcaaaccgt acattttcat
    60601 tccctcatcc attacccgcg cagttaagcg ggaaaactat ttcgcaacgt cgcgcttgtg
    60661 gctaaaatag ccgccatttt tcagctactg gataagaatg tgaccgtatc ttctcatcga
    60721 cttgaactgt taagcccggc acgcgatgcc gccattgccc gcgaagctat tttgcacggt
    60781 gccgatgctg tttatatcgg cggccctggt tttggtgccc gtcataatgc cagtaatagc
    60841 ctgaaagata ttgccgagct ggtgccgttt gcccatcgtt atggtgcaaa aattttcgtc
    60901 acgcttaaca ccattttgca tgatgatgag ctggaacccg cgcaacggct gattactgac
    60961 ctctaccaga ccggtgtcga tgcgctgatt gttcaggata tggggattct ggaacttgat
    61021 attccgccga ttgaactgca cgccagtacg cagtgcgaca ttcgtacagt tgaaaaagcg
    61081 aagttcctct ctgatgttgg cttcacgcag attgtgctgg cgcgagagct gaatcttgat
    61141 cagatccgcg cgattcacca ggctacggac gcgaccattg aattctttat tcatggggca
    61201 ctgtgcgtgg cctattcggg tcagtgctac atttctcatg cgcaaacagg gcgtagcgcc
    61261 aaccgtggcg attgctcgca ggcgtgccgt ttgccataca cattgaaaga cgatcagggg
    61321 cgggtggttt cctatgaaaa acatctgctg tcgatgaaag ataacgatca gacagtcaac
    61381 ctcggcgcgc tgattgatgc tggcgtacgc tccttcaaga ttgaagggcg ttacaaagat
    61441 atgagctacg tgaagaatat caccgcccat tatcgccaga tgctggatgc cattattgaa
    61501 gaacgtggcg atctgacgcg tgcttcatca ggtcgtactg aacatttctt tgttccatcg
    61561 acggaaaaga ctttccaccg tggtagcaca gattattttg tgaatgcccg taaaggcgat
    61621 attggcgcgt tcgattcgcc gaaatttatc ggcctgccgg tgggtgaagt attgaaagtg
    61681 gcgaaagatc atctcgatgt tgccgtcacc gagccactgg caaatggtga tggcctgaac
    61741 gtgatgatta aacgtgaagt catcggtttt cgtgccaata cggtcgagaa aaccagagaa
    61801 aatcagtacc gcgtctggcc caatgaaatg ccagcagatt tgcacaaaat tcgtccacat
    61861 cacccactaa accgtaatct tgatcataac tggcagcagg cactgacaaa aacctccagc
    61921 gaacgtcggg tggcggtaga cattgaactg ggcggctggc aggaacaact gattctgacc
    61981 ctcaccagtg aagagggtgt cagcatcacg catacgctgg acgggcagtt cgacgaagcc
    62041 aataacgccg aaaaagcaat gaacaatctg aaggatggtc tggcaaaact ggggcaaacc
    62101 ctctattacg cccgcgatgt gcaaattaat ttgccggggg cgctgtttgt accaaacagt
    62161 ctgttaaacc agttccgccg tgaagctgct gacatgctgg atgctgcgcg tcttgccagt
    62221 taccagcgcg gcagccgtaa accggttgct gatcctgcgc cggtttatcc gcaaacgcat
    62281 ctgagtttcc tcgcgaacgt atacaaccag aaagcgcgtg aattttatca tcgctatggt
    62341 gtgcagctga ttgacgcggc gtatgaagca catgaagaga agggcgaagt cccggtgatg
    62401 atcaccaagc attgtctgcg ctttgccttt aatctgtgcc cgaaacaggc gaaaggcaat
    62461 atcaaaagct ggaaggcgac gccaatgcaa ctggttaacg gcgatgaagt attaacgcta
    62521 aagtttgatt gccgcccatg cgagatgcac gtcattggca aaatcaaaaa tcacatactg
    62581 aaaatgccgt taccgggaag cgtagtggca tccgtaagtc cggatgagct gctgaaaaca
    62641 ttgcctaagc gaaaagggta aaacgccagt tttctggtta ctcacaactc attgaatctg
    62701 catgatattg cctgccgggt aaggcgtctc gccgcatccg gcaacaaatg actcaacgtt
    62761 gtcccgcttc tggtttgcgc gatttttgcc agtaaaaatg ctcgcgcaaa ccttccgccg
    62821 actcttccgc cacagcacgc aattcatcgc tgtccgcttc atgacgcagc tgatgatcca
    62881 cattctttac ccacacaaat tcatgtcctt tgtgccctgc catgagttgt cctgagaaca
    62941 gcgcacacgt taataagaca accgataacg ccttcgtaaa catcctgcca cctttttctt
    63001 accttttgcc gctatgatgc cgatcgtttc ttgaggttat tattcagttt tgcaaattag
    63061 cgcaaagaaa ttctggaatc ttccttcctg attttgcatt gcattctgcc gttgcggcga
    63121 tttagtgcta ttttcgagca aattacacac agaggtaaac actaatgttt actttgttga
    63181 tatactcagc ggcagggagg cgatgtgaaa caaagcgagt tcagacgttg gctcgaatct
    63241 cagggcgtcg atgtagcgaa tggccgcaac catttgaaac tcaggtttca tgggaggcgc
    63301 agtgtcatgc cgcgtcactc ctgcgtaaag caatcctgaa acaactcggt ttgagttaat
    63361 cgccaattaa aaaggttaat gacatgcgag agacagtcga aattatgcgt tatcccgtca
    63421 ctcttacacc cgcgccggaa ggcggttata tggtttcttt tgtggatatc cctgaagcgt
    63481 tgacgcaggg cgaaactgtc gctgaagcga tggaagcggc aaaagatgct ttactgaccg
    63541 catttgattt ttattttgaa gataacgagc ttatcccgtt accttcgcca ttaaatagtc
    63601 atgatcactt tattgaagta cctttgagcg tcgcctctaa agtattgctg ttaaatgctt
    63661 ttttacagtc agaaatcact cagcaagagt tagccaggcg aattggcaaa cctaaacagg
    63721 agattactcg cctatttaac ttgcaccatg cgacaaaaat cgacgccgtc cagcttgcgg
    63781 caaaggcgct tggcaaagag ttatcgctgg tgatggttta attacagtta acgaaaagtt
    63841 gtcattttta acaactgata tagactgccg aatcatctgc acataattac gattcgataa
    63901 tgaaaaaata ccagcagctt gcagaacaat tacgcgagca gattgcgtcg ggtatctggc
    63961 aacccggcga tcgtttgcct tcgttgcgtg accaggtggc gctttctggc atgagcttta
    64021 tgactgtcag ccatgcctat cagttgctcg aaagtctggg atatattatc gcgcgaccgc
    64081 agtcgggtta ttacgttgcg ccacaggcaa taaaaatgcc gaaagcgcca gtcattccag
    64141 tcactcgaga tgaagcagtc gatatcaaca cttatatttt tgatatgttg caggccagtc
    64201 gcgatccgtc ggtcgttccg tttgcctcgg cctttcccga cccgcgactt ttccccctcc
    64261 aacaactaaa ccgctcgctg gcgcaggtaa gcaaaaccgc cacagcgatg agcgtgattg
    64321 aaaacttacc gccaggaaac gcagaactgc gtcaggctat tgctcgtcgc tatgccttac
    64381 agggcatcac catttctcct gatgaaattg tcattactgc cggggcgtta gaggcattaa
    64441 acctcagttt gcaagcggta actgaaccgg gcgattgggt gatagtagag aatccttgtt
    64501 tctacggtgc gttgcaggcg ctggagcggc tacggctgaa ggcgttatcg gtggcgacgg
    64561 atgttaaaga agggatcgat cttcaggcgc tggaactggc gttgcaggat tatccggtga
    64621 aagcgtgctg gctgatgact aatagccaga acccactcgg atttacctta acgccgcaaa
    64681 aaaaagcaca actggtggcg ttactcaatc agtacaacgt aacgctgatt gaagatgacg
    64741 tttacagcga actttatttt ggacgggaaa aaccgctgcc tgcgaaagcg tgggatcgcc
    64801 acgatggcgt tttgcattgc tcttcgtttt cgaaatgtct ggtgcctggt tttcgtattg
    64861 gttgggtcgc cgccggaaaa catgcacgta aaattcaacg cttgcagttg atgagtacgc
    64921 tttccaccag ctcaccgatg caacttgcgc tggtggatta cctttccacg cgccgatacg
    64981 acgcccatct tcgtcgcctg cgtcgccagc ttgcggaacg taaacaacgt gcctggcagg
    65041 cactgctgcg ttatctgcct gcggaagtga aaattcatca taatgacagt ggttactttc
    65101 tctggctgga gctccccgag ccgttagatg ccggcgaatt aagcctggcg gcactgacgc
    65161 atcatatcag tattgcgccg ggtaaaatgt tttctaccgg tgaaaactgg tcacgttttt
    65221 tccgttttaa taccgcgtgg cagtggggag agcgtgaaga acaggcggta aaacaattag
    65281 gcaaacttat tcaagaacgg ctgtaatagc gtttaattta attcctctta gattgggtaa
    65341 tatgaatttc gaatagcagt catatttcct aactccttga ctatactcca gaagataacc
    65401 ttacagacgg cataatgcgc ggtagctcac aacctgatta aattttctca ggggcgaagg
    65461 tgtgcctgca agccgccgtc tatggttaaa caaggagata tttttacggc acggcggctg
    65521 aacaattaat tacgacagga gtaagacctt atgagcaaga catttgcccg cagcagcctg
    65581 tgtgcgctca gcatgacaat aatgaccgct cacgccgccg aaccgcctac caatttagat
    65641 aaaccggaag ggcgactgga tattatcgcc tggccgggat acatcgaacg cggacaaact
    65701 gataaacaat acgactgggt aacgcagttc gaaaaagaga caggctgcgc ggtgaatgtg
    65761 aaaaccgccg cgacttccga tgaaatggtc agtctgatga ccaaaggggg ttacgatctg
    65821 gttacggcat ccggcgatgc ctcgctgcgt ttgattatgg gtaaacgcgt gcagccgatt
    65881 aataccgcat tgattcccaa ctggaaaacg ctcgatccgc gcgtggttaa aggcgactgg
    65941 tttaatgttg gcggcaaagt ttacggcaca ccttaccaat gggggccgaa cctgctgatg
    66001 tacaacacta aaaccttccc gacgccgccg gatagctggc aagtggtttt tgttgagcaa
    66061 aatctgccgg acggcaagag caataaaggc cgcgttcagg cttatgatgg ccctatctac
    66121 attgcggacg ctgcgttgtt cgttaaagcc actcagccgc agttgggcat cagcgatccg
    66181 tatcaactca ccgaagaaca gtaccaggcg gtgctgaaag tgctgcgcgc tcaacacagt
    66241 ttgatccatc gctactggca tgacactacc gtgcaaatga gcgatttcaa aaacgagggt
    66301 gtggttgctt ccagtgcctg gccctatcag gccaacgccc tgaaagccga aggccagcct
    66361 gttgctaccg ttttcccgaa ggagggtgtt accggttggg ctgataccac catgctgcat
    66421 agcgaagcga aacatccggt ttgcgcctac aaatggatga actggtcatt aacgccaaaa
    66481 gtgcagggcg atgtggcggc ctggtttggc tcgttaccgg tagtgccgga agggtgtaaa
    66541 gccagtccgt tattaggcga aaaaggttgt gaaaccaacg gttttaacta tttcgacaaa
    66601 atcgccttct ggaaaacgcc tatagcagaa gggggcaagt ttgttcccta cagtcgctgg
    66661 acgcaggatt acattgccat tatgggcggt cgctaacttc gctggggtgc tttatgacgt
    66721 acgcagtgga gtttgacaac gtctcgcggt tgtacggtga cgtgcgcgca gtagatggcg
    66781 tcagtattgc gataaaagat ggtgagttct tctctatgct ggggccgtcc ggctccggca
    66841 aaaccacctg cctgcgcctg attgctggct tcgaacagct ttccggcggg gctatctcta
    66901 tctttggtaa acccgccagc aatctgccac cgtgggagcg ggacgtgaat actgtctttc
    66961 aggactacgc gctatttccg catatgtcga ttcttgacaa tgtcgcctat gggctgatgg
    67021 tcaaaggcgt gaataaaaag cagcggcacg caatggcgca agaggcgctg gagaaagtgg
    67081 cgttggggtt tgtacatcaa cgtaaaccgt cacaactttc tggtggtcag cgccagcggg
    67141 ttgctatcgc cagagcattg gtgaatgaac cgcgcgtatt gctgttggat gaaccgctcg
    67201 gcgcactgga tctcaaattg cgtgagcaga tgcagctgga actgaaaaaa ctgcaacagt
    67261 ctctcggtat cacttttatc ttcgttaccc acgatcaggg cgaagcgtta tcgatgtccg
    67321 atcgtgtggc ggttttcaat aatggacgca ttgagcaggt cgattccccg cgcgatctct
    67381 atatgcgccc gcgcacgccg tttgttgccg gattcgtcgg gacatccaac gtttttgatg
    67441 gactgatggc agagaaactt tgtggcatga cgggaagctt cgccctgcga ccggaacata
    67501 tccgcctcaa cacccctggt gaactgcagg ccaatggcac gatccaggcg gtgcaatatc
    67561 agggcgcggc aactcgtttt gaactgaaat tgaacggcgg tgaaaaactg cttgtgagtc
    67621 aggccaatat gacaggcgaa gaactgcctg ccacgctcac gcccggacaa caggtgatgg
    67681 tttcctggtc gcgtgatgtg atggtgccgc tggttgagga gaggtgaatg gcgatgaatg
    67741 tattgcaatc accttcacgt ccaggtctgg gtaaggtgtc cggtttcttc tggcgtaatc
    67801 cggggctggg gctgttttta ctgctgcttg gcccgctaat gtggtttggc attgtctatt
    67861 tcggctcgct gctgacactg ttatggcagg gattttatac ttttgacgat ttcaccatgt
    67921 cggtaacgcc ggaactgacg ctggcgaata tccgtgcgct gtttaatccg gcgaattacg
    67981 acatcattct ccgcacgctg actatggctg tggcagtcac tatcgccagc gccattctgg
    68041 cttttccaat ggcgtggtat atggcgcgct ataccagcgg gaaaatgaaa gctttttttt
    68101 atattgcggt aatgttgccg atgtgggcga gctacattgt taaagcctat gcctggacgt
    68161 tattgctggc aaaagatggc gtggctcagt ggtttttaca acatcttggg ctggaaccac
    68221 tgctgacggc gttccttaca ttacctgcgg tgggcggaaa tacgctgtca acttccgggc
    68281 tggggcgctt tctggtgttt ctctatatct ggttgccgtt catgatcctg cccgttcagg
    68341 cggcgcttga gcgtttgccg ccgtcattgt tgcaggcgtc ggctgatctc ggcgcacgtc
    68401 cacgacaaac ctttcgctat gtggtgctgc cgctggcaat cccgggtatt gccgctggct
    68461 ctatctttac cttctcactc acactgggcg attttatcgt cccgcagctg gttggtcctc
    68521 caggatattt tatcggcaat atggtttatt cccagcaggg ggcgattggc aatatgccga
    68581 tggcggcggc attcaccctg gtgccgattg ttctcatcgc actgtacctg gcgttcgtga
    68641 aacgtctggg agcgttcgat gcactctgaa cgcgcaccgt ttttcctcaa actggcggcc
    68701 tggggcggcg tggttttcct acattttccc atcctgatta tcgccgctta tgcgtttaac
    68761 actgaagatg cggcgtttag ttttccaccg cagggcctga cgctgcgctg gtttagcgtg
    68821 gcagcacagc gtagtgatat tcttgatgcc gtgacactgt cacttaaagt ggcggcgctg
    68881 gcgacattaa tggcgctggt gttagggacg ctggcaactg ccgcgctgtg gcgacgagac
    68941 tttttcggca aaaacgccat ttcgctgtta ctgctgctgc ccattgcgct gccgggcatt
    69001 gtcactggtc tggcgctatt aaccgctttt aaaaccatca atctggagcc gggatttttc
    69061 accatcgtgg tcggtcatgc gactttttgc gtagtggtgg tgtttaacaa tgtcatcgcc
    69121 cgttttcgcc gcacctcgtg gagtctggtt gaggcgtcaa tggatcttgg ggccaatggc
    69181 tggcaaacct tccgctacgt agtgttgccg aatctcagtt cggcgctact ggcaggagga
    69241 atgctggcgt ttgccttgtc gttcgatgaa atcatcgtta cgacttttac ggcaggtcat
    69301 gaacgaacgt taccgttgtg gttgctcaat cagcttgggc gaccgcgtga tgtaccggta
    69361 actaacgtgg tggcactgct ggttatgttg gtaacaacct tgccgatcct gggggcctgg
    69421 tggctaaccc gcgaaggcga caatggtcaa taaccactga tacaggaata tgctatgcaa
    69481 cataagttac tgattaacgg agaactggtt agcggcgaag gggaaaaaca gcctgtctat
    69541 aatccggcaa cgggggacgt tttactggag attgccgagg catccgcaga gcaggtcgat
    69601 gctgctgtgc gcgcggcaga tgcagcattt gccgaatggg ggcaaaccac gccgaaagtg
    69661 cgtgcggaat gtctgctgaa actggctgat gttatcgaag aaaatggtca ggtttttgcc
    69721 gaactggagt cccgtaattg tggcaaaccg ctgcatagtg cgttcaatga tgaaatcccg
    69781 gcgattgtcg atgtttttcg ctttttcgcg ggtgcggcgc gctgtctgaa tggtctggcg
    69841 gcaggtgaat atcttgaagg tcatacttcg atgatccgtc gcgatccgtt gggggtcgtg
    69901 gcttctatcg caccgtggaa ttatccgctg atgatggcag cgtggaaact ggctccggcg
    69961 ctggcggcag ggaactgcgt agtgcttaaa ccatcagaaa ttaccccgct gaccgcgttg
    70021 aagttggcag agctggcgaa agatatcttc ccggcaggcg tgattaacgt actgtttggc
    70081 agaggcaaaa cggtgggtga tccgctgacc ggtcatccca aagtgcggat ggtgtcgctg
    70141 acgggctcta tcgccaccgg cgagcacatc atcagccata ccgcgtcgtc cattaagcgt
    70201 actcatatgg aacttggtgg caaagcgcca gtgattgttt ttgatgatgc ggatattgaa
    70261 gcagtggtcg aaggtgtacg tacatttggc tattacaatg ctggacagga ttgtactgcg
    70321 gcttgtcgga tctacgcgca aaaaggcatt tacgatacgc tggtggaaaa actgggtgct
    70381 gcggtggcaa cgttaaaatc tggtgcgccg gatgacgagt ctacggagct tggaccttta
    70441 agctcgctgg cgcatctcga acgcgtcagc aaggcagtag aagaggcgaa agcgacaggg
    70501 cacatcaaag tgatcactgg cggtgaaaag cgcaagggta atggctatta ctatgcgccg
    70561 acgctgctgg ctggcgcatt acaggacgat gccatcgtgc aaaaagaggt atttggtcca
    70621 gtagtgagtg ttacgccctt cgacaacgaa gaacaggtgg tgaactgggc gaatgacagc
    70681 cagtacggac ttgcatcttc ggtatggacg aaagatgtgg gcagggcgca tcgcgtcagc
    70741 gcacggctgc aatatggttg tacctgggtc aatacccatt tcatgctggt aagtgaaatg
    70801 ccgcacggtg ggcagaaact ttctggttac ggcaaggata tgtcacttta tgggctggag
    70861 gattacaccg tcgtccgcca cgtcatggtt aaacattaag ttagatgtta gtggctgtta
    70921 taggacggcc actatcgttt taggttctga gtttaatggc ggttaagtat tagaatgatt
    70981 acattaataa ctagcgtaaa aattaggcta aatgtttgat ggcgattttt catcctgtca
    71041 cagttgatta gcataaggct ttagagtatc gttattaaac taataattta taagtagaag
    71101 tattaacaat gaaagttcaa ttgtacaaat tcacggaaga taaaaataaa acccttacct
    71161 tcagatggac gaaaaaacac tttgagtttt gtatggataa taaaatcttt ttaaatcata
    71221 aggggaagaa gtcgtataaa gaaaggaatt tatttctttt ctcaaaaggt gacaagataa
    71281 caattgaaga taatgttatt gctgaagaat attcaacaat gcctgtgaaa aatttctcca
    71341 gtgtcggcgc tttttcattt ccgacctgcc atttttcagg gaatatacgt ataggtcgat
    71401 tttgtagtat tgcatcaaat gtaaaaataa tgggtggaaa tcatcctttg aatcgtttta
    71461 ctactcacat gatgacttat aacggtgaat ttgataagtt tgctatgagt gaatttgaac
    71521 gtagctggac attgaaacca ttcattacca aacctgaaaa tccgattata ggaaatgatg
    71581 tctggatagg aaatgatgtt gtattgaaag gtggcatcgc aataggtgat ggtgctgtta
    71641 ttgctgccaa ttccgttgtt accaaagatg tgcccccata tgccatagtt gcaggagttc
    71701 ctgcaaaaat aatcagattt aggtttgatt caaatgttat tgatgaactg ttgagaataa
    71761 aatggtggaa ttataactac tcagatcttc cggataataa caagtgtgat gatataaatt
    71821 attttgttga agagatgaat cgattgatat cagatggaaa tattcaagag agggactata
    71881 aaaaattcaa tctttctgag gtattcagag gcctataatt atctgttttg catgataaat
    71941 gggcaagata accagggcgg ggtttgcaat ttcaatgggg cagcgtcatc aaggaggttg
    72001 caaacatcct gccatgcata tactttacgc catcttttta tatttacgtt attccttgaa
    72061 agtataaagc atttatagac tcccgcgcgt ataaagtatt catgatagaa aaatgtgaaa
    72121 acaagattcc gtttgaatca gtaagctgtt tgtatagacg acagtagggg gatgttttct
    72181 actcatttca atgtagatgc cagcaaaaag aagtaaatat taaggataat gttgtaaacc
    72241 tggagagtat gagaagatct tgcgtactgt cagagagttg atttctctga cagtatgtgc
    72301 tgcgtgcaaa atcactacat caaataaacc agccaaatct catagagata agagtaaaca
    72361 aaccaacagc agcaaagaca tttatcagta ctacagttct actggaaaca ttcatttttg
    72421 ccacctttta tcattgcccc tatgtgtata gtacgggtta agaaaatccg caataatact
    72481 cattctgaag atggataagg gcaagttgct gtttgatgat tttttctcaa actggtgaaa
    72541 aaacttgatg cacgtcaaaa aatgacgcat atttgcgcgc gttttattca tctggctgga
    72601 cgcccgtaca tgtctctcta tcaacatatg cttgtttttt atgcggttat ggcagcaatc
    72661 gcatttctta tcacctggtt tctttctcac gataagaaac gcatccgttt cttaagcgcc
    72721 tttctggtgg gggcaacatg gccgatgagt ttcccggtgg cgctgttgtt ttcactgttt
    72781 taaatcttac tcactggctt gacggcacca cgcagactta tatcatttgg atgaatcgat
    72841 aaatttcaca agtggttaag gagaaagtat gtcgcatctg gatgaagtca tcgcgcgcgt
    72901 ggatgccgcg attgaagaga gcgtcattgc ccatatgaac gaattattaa tcgccctgag
    72961 cgatgacgcg gagttaagtc gggaagatcg ctacacccag cagcagcgtt tgcgcaccgc
    73021 tattgctcat cacggtcgca agcataaaga agatatggaa gcgcgccacg aacagttaac
    73081 caaaggcggc accatcctct gattaaaatg agcgtctggc taccaaccag gcgccaacca
    73141 caagtaaaat tgcgccacaa attgggccga acaacgcccg tagtggtacg ccatgactgc
    73201 ggaagatatc cagcatcaga ccgccaatta gctggctggc gaccagtact gcaatagttg
    73261 tcgccgctcc cacattctga tatccgctga tactggcgaa gacaaaaaac gatcccagca
    73321 agccgggtat aagtgtccac cagcgcacgc tggacaccag ttcgccaaac cccgccatgc
    73381 cttgtttaaa ccataaaata gaaacaaaga gcacaatccc caccagtgag ttcagcaaca
    73441 tggcgatgag aatggtagag gaggtctggg tgatgcgcac catcagcgtg ttctggacta
    73501 ccagaccaat tccggcggca atcaaaaaag caagggtgag cgactgattc atccaatcgc
    73561 gtccggttca gtgcgttcgt cgagttgcaa ctgcataaat gtcagatcca gccagcgccc
    73621 aaatttagtg cccacctgcg gcatttgtgc ggtgacgaca aatcccagcg actggtgcag
    73681 atgcagcgag gcctgatttt gcgattcgat cccggcgacc atgacatgct tcccgcaatc
    73741 ccgcgcttca tcaatcaatc ggcttaacaa tttacgcccc agacctttgc cctgatgatc
    73801 gggatggaca taaaccgaat gttccacggt atggcgaaaa ccatcgaaac tacgccagtc
    73861 gccaaacgag gcatatcccg tcactacgct atcttcttca cttaccagca ccggataacc
    73921 tgctatggtc cgcgcttcaa accaggcaat gcgattatct gcatccaccg tttggtcatt
    73981 ccagatagcc gccgtataca acacggcgtg gttataaatt tccgcaatgg cagcacagtc
    74041 ggctttgcgg gcaaaacgga tggacatgtc tggcctcaat aaaataatga tgatgatgtt
    74101 atcaatacta tcgaaaacag cccgaagcaa cggattcctc gatgaaatta ttgcgtgttg
    74161 cagaggggat gtgacggctg caaacaaaac caatcctgcg agcctgctac tattgatgga
    74221 ttcatgccaa aagcacagga gagcattatg gggcaacaaa agcagcgtaa ccgccgttgg
    74281 gttctggcct cgcgtccaca tggcgcaccg gttccggaga atttccgtct tgaagaagat
    74341 gatgtcgcca caccgggtga aggacaggtg ttactgcgca cagtttattt gtccctggac
    74401 ccgtatatgc gtggacgtat gagcgatgag ccatcttatt caccgcctgt tgatattggc
    74461 ggcgtgatgg tcggcggcac ggtgagccgt gttgtggagt cgaatcatcc tgattatcag
    74521 cctggcgact gggtgctggg ctacagtgga tggcaggact atgacatatc cagtggtgat
    74581 gatctggtga aacttggcga tcatccgcaa aatccatcgt ggtcgctggg tgtgctgggg
    74641 atgccaggct ttaccgctta tatggggctg ctggatatcg gtcagcctaa agagggcgaa
    74701 acgttggtgg tagctgcggc gacaggtcct gtgggagcaa cggtggggca aatcggcaaa
    74761 cttaaaggtt gcagggtggt gggggttgcc ggtggcgcgg aaaaatgccg ccatgctatc
    74821 gaggtgctgg gcttcgatgt ttgtcttgat caccacgcgg atgattttgc cgaacaactg
    74881 gtgaaagcgt gcccaaaagg tattgatatc tattatgaaa acgtgggcgg taaggtattt
    74941 gatgcagtgc taccattact taatacatct gcgcgcattc ccgtttgtgg cttagtgagc
    75001 agctataacg ctacagagct accacccggt ccggatcgtt tacccctgtt gatggcaacg
    75061 gtgctgaaaa aacgtattcg cttgcaaggg tttattatcg ctcaggatta tggtcaccgc
    75121 atccatgagt ttcagaagga gatggggcaa tgggtgaaag aggataaaat ccactaccac
    75181 gaagatatta ctgatggttt agagaatgcg ccacagacgt ttatcggcct gctgaagggt
    75241 aaaaacttcg gcaaagtggt gatccgcgtc gcgggtgatg attaaagttt caacggcggc
    75301 gtaagccgcc ataaaaatcc aggcaaaaag cttattttca gctttaatta actaaccacg
    75361 tcattgttct gctgctaata acaacaaagg tgggtggttt atgattattc actttaatac
    75421 accaggtgaa ctccttctgc cgtgcaggca gggttggaca gaaaacacgc gaaggaatta
    75481 aaatgcctgg aatgggaaaa atgaaacatg tcagtttgac tctgcaggtt gagaacgacc
    75541 tgaaacatca gcttagtatt ggcgcattaa aacctggcgc acgcctgatt actaaaaatc
    75601 tggcggagca attaggtatg agtattacac ctgtgcgtga agcattatta cgtctggttt
    75661 cggtgaatgc gctttctgtc gcacctgcac aagcatttac agttccggaa gtgaggaaac
    75721 gtcaattaga cgaaatcaat cggatccgct acgaactgga attaatggca gttgctctgg
    75781 ctgttgaaaa cctcaccccg caagacctgg cggaactcca ggaattgctc gagaaattac
    75841 agcaggcgca agaaaagggc gacatggaac aaatcattaa tgtaaacagg ctatttcgct
    75901 tagcgattta tcatcgctca aatatgccca tcctgtgtga gatgattgag caactgtggg
    75961 tcaggatggg gcctggttta cattatctct atgaagcgat taatccagcg gaattacggg
    76021 agcatataga aaactatcat ctgttactcg ctgcgttaaa agcaaaagac aaagagggat
    76081 gcagacattg tcttgctgaa attatgcaac aaaatattgc tattttatac cagcaataca
    76141 atcgttaaag taaatgcccg gcaaataccg ggcatcaagg taattactca aatctccacg
    76201 caatattcat gccgacacca taatttcgtc cgggcgaagg ttcgtaatat cgcccgtttg
    76261 actcattgac aatgacagaa ccaacgtatt ctttatcgaa taaattatcg acacgaccaa
    76321 ataaatccac agttaaattg tggtaattgt atttataccc ggtgaataag ccgacgagag
    76381 tataagacgg cgcttttgcc gtattttcat catctgccat aatatcgccc atataacgcg
    76441 cttccgtgcc tgcataccaa ccatcttccg gtacataacc tatcgacgca aagcccatat
    76501 tacgggcgat ccctggcatc cgattaccgt tacaatcctg ttcattgcaa acattgctgc
    76561 gataggtcgc atcaagccag gtccaggacg cgtttacgcg aaaatctcct gcgaaacgtt
    76621 gatcccatgc cagttcagcg ccttgacgac gggtctttcc ggcatttttg taagtcgtac
    76681 gcccaccgct actgctatcg acaacaattt catcatcagt gtcggtctga aacaatgcga
    76741 gactaagcag cccatcacca atacgcgttt tactgccgat ctcaattgta tcgttggtgg
    76801 atggttttaa accaaagttc ataccgcttt gcccatcagc acgataagac agctcattaa
    76861 tcgtcggcgt ttcaaaacct cgcccggctg ccagatagat attccaggca tcggtcattg
    76921 catattttaa cgaaccggca ggtagccatt tatgataact ggcatcaccg ctgtcatcgc
    76981 cgttacccgg agtaacgtaa tggtcgttgg aatcaaacca cacggagctg tagcgcacgc
    77041 cagcatccag cgacagtttt tcgctcagct gccactgcgt ctgtaaatag ggatcgatgt
    77101 tccacatcag attgcgttcg tcgcgacgca actcaccttt ttgcccgtac tccggcatgc
    77161 cgctattcag gcggaagtta ttgtagccct tgcggttttc actcatgttt tcgtagttca
    77221 ggccggtagt gaacgtgacc ggaacgccca gttcaccacg gtgtgtccag cggctgtcta
    77281 tcccctggta atggcgttgc agggtaatca cgccgcccgc atgtgacggg ttaagttgtg
    77341 gtgccatggg tattgactgg tactgggtcg tttctcgctc tccggcatac atcatcacac
    77401 tcatatcatc ttgcgcgctc aggctacgct catagcgcaa cccagcctga gtttgcttga
    77461 tggtttttcg cgtatcgtac tgttctgcac gaggcgcttg ttgtggatta gccttccatt
    77521 ctgctttggt tagcccacct gggtcatctg ctttgatatc cacactattg aaaatcagac
    77581 ttaatttgct ggcttcatca atgcgtacgc ccagtttggc attagctaaa tttttctgtg
    77641 cgccactatg gtcacgatag ccgtgggtcg taaaacgcgt ggttgagacg gtgtaatcga
    77701 catcgccagg ctgtgtgccg tctcccgttg cgcccgttgc tttcagccca tagcgccagc
    77761 tgccaaaact gccgtagtaa ctactggctt caatggttgg tggctgttgt ccggtctggg
    77821 tggtgacatt cattacccca ccagacgcgt tgccatacag ggcagagaag gggccacgca
    77881 gcacttccac attttgcaca ctgcttaaat cgatgttgga tgtttgccct tgcccgtcgg
    77941 gcatggtggc gggaataccg tccacataca ggcgaatacc gcgaataccg taagtggagc
    78001 gggagccaaa tccgcgaatc gacagctgta aatcttgcgc atagttctgc cggttttgta
    78061 cctgcaaacc aggcacgccg gtcagtgatt cggacaagtt aatgcgcgat gttgccaggc
    78121 gcatctcctc gccatccacc acgcttactg ctgctggggt atccagttct gaaaccacct
    78181 gcggtgcggc actgacaatc atagtctgtt catcagcggc aaaaacaacg ggggaaagga
    78241 caagcagtgc gggcaaaacg gtctgtcgga cggaaaaaat cttcatgaaa aaagccaggt
    78301 taagaatggg aaaacgctgt catggtaata aaattgtaaa tttatggaaa acgaaacggc
    78361 acgatacgtt aattaattga gaaaattgta gtcgtaacgg caagaaatgc tccacatttg
    78421 agaaaataat gattaccatt cccatttata acaagagcgt aacgatgatt acgcttagcg
    78481 aagcattgtg aagcagcaaa aatattggtt catcaaaggg agtcgtcatg catttacgtc
    78541 atctgttttc atcgcgcctg cgtggttcat tactgttagg ttcattgctt gttgcttcat
    78601 cattcagtac gcaggccgca gaagaaatgc tgcgtaaagc ggtaggtaaa ggtgcctacg
    78661 aaatggctta tagccagcaa gaaaacgcgc tgtggctcgc cacttcgcaa agccgcaaac
    78721 tggataaagg tggcgtggtt tatcgtcttg atccggtcac tctggaagtg acgcaggcga
    78781 tccataacga tctcaagccg tttggtgcca ccatcaataa cacgactcag acgttgtggt
    78841 ttggtaacac cgtaaacagc gcggtcacgg cgatagatgc caaaacgggc gaagtgaaag
    78901 gccgtctggt gctggatgat cgtaagcgca cggaagaggt gcgcccgctg caaccgcgtg
    78961 agctggtagc tgatgatgcc acgaacaccg tttacatcag tggtattggt aaagatagcg
    79021 tgatttgggt cgttgatggc gagaatatca aactgaaaac cgccatccag aacaccggta
    79081 aaatgagtac cggtctggcg ctggatagca aaggcaaacg tctttacacc actaacgctg
    79141 acggcgaatt gattaccatc gacaccgccg acaataaaat cctcagccgt aaaaagctgc
    79201 tggatgacgg caaagagcac ttctttatca acattagcct tgataccgcc aggcagcgtg
    79261 catttatcac cgattctaaa gccgcagaag tgttagtggt cgatacccgt aatggcaata
    79321 ttctggcgaa ggttgcggca ccggaatcac tggctgtgct gtttaaccca gcgcgtaatg
    79381 aagcctacgt aacgcatcgt caggcaggta aagttagtgt gattgacgcg aaaagctata
    79441 aagtggtgaa aacgttcgat acgccgactc atccaaacag cctggcgctg tctgccgatg
    79501 gcaaaacgct gtatgtcagt gtgaaacaaa aatccactaa acagcaggaa gctacccagc
    79561 cagacgatgt gattcgtatt gcgctgtaat agatatggcg agggtgaaaa cggagagact
    79621 tgcctgactt ttatcacgcc gcatccggca ccttcgtcag taatctgaat ttgcccggca
    79681 cgtagccggg caattttgca attaactcgc cgtttcaggc ttaaacacaa tttcctgttt
    79741 ttcttcatct tcctcgacga ctggcgcagt gctgtgaatt tcagcaacgc gtttgcgcac
    79801 accaaaccag cctataacca gcagaatacc gataatcggc agcgccgcga tagtataagt
    79861 cccgttcggg taatcgaacg ccatcagcac caggacactc agtaaaaaca gtaatgtcag
    79921 ccaggaagtg aagggcgcgc caggcagttt aaaactgaca tccgctgctt tgccttgttt
    79981 aattgcttta cgcaggcgca tctggcacac gatgataaac gcccatgaag cgatgattcc
    80041 cagcgacgcg aagttcaaca caatctcaaa tacgcgcgac ggcaccagat agttgaggaa
    80101 tacgccgacg acatacacaa ccagtgtcgc cagaatcccg gcatacggca catgctgacg
    80161 actcattttc gccataaaac tcggtgcgga accgcccatc gccatcgagc gcagaatacg
    80221 cccggtgcag tacaggcctg agttcaggct ggagagggca gcggtcagca ccacaatgtt
    80281 cataatgctg ccgatatatg gcacacccag tttagagaaa aacgtcacga acggactttg
    80341 ccccgcctga tacgcgctcc acggcaataa cataaccagc aacaccacgg agccgacgta
    80401 aaacaggcca atacgccaaa tcacactgtt aatcgcttta ggcaccatgg tctgcggatc
    80461 tttacattct ccggcagctg tacccaccat ttcaatggag gcaaaagcaa acactacgcc
    80521 ctgaatcaac accagcgcag gcagcaaacc gtgggggaag aagccgccat tatcggtgat
    80581 taaatgaaag ccagtggtgt tgccatccag cggctgaccg ctaccgagga acactgtacc
    80641 cacgaccaaa aaggtcacaa tggcgagcac tttaataagc gcaaaccaga actccatctc
    80701 cgcaaaccat ttcacaccga tcatattcat ggtgccaacg atggtcagcg cagcgagcgc
    80761 aaagacccac tgcggcacgc cgccaaacgc accccagtaa tgcatataca gagcgacggc
    80821 ggtaatatca acaatccccg tcatcgccca gttgatgaag tacatccagc cagcaacata
    80881 agcggctttc tcacccaaaa actcacgggc ataagagaca aaactgccac tggaagggcg
    80941 gtgtagcacc agctcaccca atgcacgcag aataaaaaac gaaaacaagc cacaaattaa
    81001 ataaaccagt gccagtgctg gacccgccat ttgcagtcgg gctcctgcac ctaaaaacaa
    81061 gccggtgcca atcgcgccgc caatggcgat catctgtacc tggcgattgc ccatcgcttt
    81121 gtgatacccc tcttcgtggg cattaagcca gcggcgtttc gcggcgtgtt gatctgaagt
    81181 gtcggtgtcg tgtttactca ttgctctccc tgattgcttt aatgaaaaag tcatataagt
    81241 tgccatgaac aatgtttatt catcctggcg atgatagtta tcaccattat tctttatggg
    81301 tataaaacta tcggcgcagc atcctacccg ttatcgataa acgatgcaaa acatcccctt
    81361 acaatcctga aggggattaa tacaactgac gaaaaaatga caaatccttt tgctggttaa
    81421 cctgtgtact gtcctacact taatctttaa aagattgtga ggggcatatg attaaggtgt
    81481 atggcgtacc cggatggggc tctacaatca gtgagctgat gctaacactg gctgatattc
    81541 cttatcagtt cgttgatgtc agtggttttg accacgaggg aacttcacgc gagttattga
    81601 aaaccctgaa cccgttgtgt cagattccga cactggcgct ggaaaatgac gagattatga
    81661 cggagacagc ggcgattgca ttgatggttc tcgatcgtcg cccggacctt gcgccaccgg
    81721 ttgggcgcgc cgaacgtcag cagtttcaac ggctattggt ctggctggtt gctaatgtct
    81781 atccaacatt cactttcgcc gattaccctg agcgttgggc tcctgacgca cctgaacagt
    81841 taaagaagaa tgttattgaa tatcgtaaat cgctttatat ctggctgaat tcacaattga
    81901 ccgctgaacc ttatgcgttt ggtgagcaac taacgctggt ggactgttat ctttgcacta
    81961 tgcgcacatg ggggcctggg catgaatggt ttcaggataa tgccacgaat atcagtgcga
    82021 ttgctgatgc tgtatgtcag ttaccgaaat tacaagaagt attaaaaagg aatgaaatta
    82081 tttgagtggg ataccagagg attatgttat ttattattca gtaactttat ccacaatgat
    82141 gggcgtaatt aattaaattc atgttatgtt ttttgattta tatcactctc cccttcattc
    82201 agcttgtcta tttcatttcc tctgaaaaga aactaactat tcacattgtg caga
//
LOCUS       DAZFXT010000007        80189 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.007, whole genome shotgun sequence.
ACCESSION   DAZFXT010000007 DAZFXT010000000
VERSION     DAZFXT010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 80189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.007"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            92..676
                     /gene="yraH"
                     /locus_tag="ACHKW0_000498"
     CDS             92..676
                     /gene="yraH"
                     /locus_tag="ACHKW0_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7473386.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            756..1451
                     /gene="yraI"
                     /locus_tag="ACHKW0_000499"
     CDS             756..1451
                     /gene="yraI"
                     /locus_tag="ACHKW0_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7473387.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1481..3997
                     /gene="yraJ"
                     /locus_tag="ACHKW0_000500"
     CDS             1481..3997
                     /gene="yraJ"
                     /locus_tag="ACHKW0_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7473388.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFNEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALYL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            4008..5099
                     /gene="yraK"
                     /locus_tag="ACHKW0_000501"
     CDS             4008..5099
                     /gene="yraK"
                     /locus_tag="ACHKW0_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7473389.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5142..6002)
                     /gene="rsmI"
                     /locus_tag="ACHKW0_000502"
     CDS             complement(5142..6002)
                     /gene="rsmI"
                     /locus_tag="ACHKW0_000502"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HFO7473390.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGICVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIIEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6066..8054
                     /gene="lpoA"
                     /locus_tag="ACHKW0_000503"
     CDS             6066..8054
                     /gene="lpoA"
                     /locus_tag="ACHKW0_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HFO7473391.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTTQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PAPVSTPAATTTAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATLYASSRSAQGTAGPDFR
                     LEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMGVDAWSLANHFSQMRQV
                     QGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8012..8407
                     /gene="yraN"
                     /locus_tag="ACHKW0_000504"
     CDS             8012..8407
                     /gene="yraN"
                     /locus_tag="ACHKW0_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HFO7473392.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8427..9017
                     /gene="diaA"
                     /locus_tag="ACHKW0_000505"
     CDS             8427..9017
                     /gene="diaA"
                     /locus_tag="ACHKW0_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001483830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HFO7473393.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDV"
     gene            9027..9602
                     /gene="dolP"
                     /locus_tag="ACHKW0_000506"
     CDS             9027..9602
                     /gene="dolP"
                     /locus_tag="ACHKW0_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006970 - response to osmotic stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/outer membrane stress-associated
                     lipid-binding lipoprotein"
                     /protein_id="HFO7473394.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKEARINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9716..10756)
                     /gene="yraQ"
                     /locus_tag="ACHKW0_000507"
     CDS             complement(9716..10756)
                     /gene="yraQ"
                     /locus_tag="ACHKW0_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7473395.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVMAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10829..11464)
                     /gene="yraR"
                     /locus_tag="ACHKW0_000508"
     CDS             complement(10829..11464)
                     /gene="yraR"
                     /locus_tag="ACHKW0_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HFO7473396.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLTDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPGHEGVTILSSSELRKRAE"
     gene            11592..12110
                     /gene="yhbO"
                     /locus_tag="ACHKW0_000509"
     CDS             11592..12110
                     /gene="yhbO"
                     /locus_tag="ACHKW0_000509"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HFO7473397.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12090..12533)
                     /gene="yhbP"
                     /locus_tag="ACHKW0_000510"
     CDS             complement(12090..12533)
                     /gene="yhbP"
                     /locus_tag="ACHKW0_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HFO7473398.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRGSGTEQA"
     gene            12584..12886
                     /gene="yhbQ"
                     /locus_tag="ACHKW0_000511"
     CDS             12584..12886
                     /gene="yhbQ"
                     /locus_tag="ACHKW0_000511"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HFO7473399.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAESAGFAELLSSLQTPEIKSD"
     gene            complement(12873..13376)
                     /gene="yhbS"
                     /locus_tag="ACHKW0_000512"
     CDS             complement(12873..13376)
                     /gene="yhbS"
                     /locus_tag="ACHKW0_000512"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7473400.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13370..13894)
                     /gene="ubiT"
                     /locus_tag="ACHKW0_000513"
     CDS             complement(13370..13894)
                     /gene="ubiT"
                     /locus_tag="ACHKW0_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HFO7473401.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14103..15098
                     /gene="ubiU"
                     /locus_tag="ACHKW0_000514"
     CDS             14103..15098
                     /gene="ubiU"
                     /locus_tag="ACHKW0_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HFO7473402.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFIPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15107..15985
                     /gene="ubiV"
                     /locus_tag="ACHKW0_000515"
     CDS             15107..15985
                     /gene="ubiV"
                     /locus_tag="ACHKW0_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HFO7473403.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAICSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16191..17198
                     /gene="yhbW"
                     /locus_tag="ACHKW0_000516"
     CDS             16191..17198
                     /gene="yhbW"
                     /locus_tag="ACHKW0_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HFO7473404.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17316..18560)
                     /gene="mtr"
                     /locus_tag="ACHKW0_000517"
     CDS             complement(17316..18560)
                     /gene="mtr"
                     /locus_tag="ACHKW0_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HFO7473405.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18714..20603)
                     /gene="deaD"
                     /locus_tag="ACHKW0_000518"
     CDS             complement(18714..20603)
                     /gene="deaD"
                     /locus_tag="ACHKW0_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HFO7473406.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20596..20676)
                     /gene="yrbN"
                     /locus_tag="ACHKW0_000519"
     CDS             complement(20596..20676)
                     /gene="yrbN"
                     /locus_tag="ACHKW0_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HFO7473407.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20783..21667)
                     /gene="nlpI"
                     /locus_tag="ACHKW0_000520"
     CDS             complement(20783..21667)
                     /gene="nlpI"
                     /locus_tag="ACHKW0_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HFO7473408.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21776..23911)
                     /gene="pnp"
                     /locus_tag="ACHKW0_000521"
     CDS             complement(21776..23911)
                     /gene="pnp"
                     /locus_tag="ACHKW0_000521"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HFO7473409.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24158..24427)
                     /gene="rpsO"
                     /locus_tag="ACHKW0_000522"
     CDS             complement(24158..24427)
                     /gene="rpsO"
                     /locus_tag="ACHKW0_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HFO7473410.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24576..25520)
                     /gene="truB"
                     /locus_tag="ACHKW0_000523"
     CDS             complement(24576..25520)
                     /gene="truB"
                     /locus_tag="ACHKW0_000523"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HFO7473411.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25520..25921)
                     /gene="rbfA"
                     /locus_tag="ACHKW0_000524"
     CDS             complement(25520..25921)
                     /gene="rbfA"
                     /locus_tag="ACHKW0_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HFO7473412.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26085..28757)
                     /gene="infB"
                     /locus_tag="ACHKW0_000525"
     CDS             complement(26085..28757)
                     /gene="infB"
                     /locus_tag="ACHKW0_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HFO7473413.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28782..30269)
                     /gene="nusA"
                     /locus_tag="ACHKW0_000526"
     CDS             complement(28782..30269)
                     /gene="nusA"
                     /locus_tag="ACHKW0_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HFO7473414.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQALEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30297..30749)
                     /gene="rimP"
                     /locus_tag="ACHKW0_000527"
     CDS             complement(30297..30749)
                     /gene="rimP"
                     /locus_tag="ACHKW0_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HFO7473415.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30956..31032)
                     /locus_tag="ACHKW0_000528"
     tRNA            complement(30956..31032)
                     /locus_tag="ACHKW0_000528"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30996..30998),aa:Met,seq:cat)
     gene            31380..32723
                     /gene="argG"
                     /locus_tag="ACHKW0_000529"
     CDS             31380..32723
                     /gene="argG"
                     /locus_tag="ACHKW0_000529"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HFO7473416.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(32731..34356)
                     /gene="yhbX"
                     /locus_tag="ACHKW0_000530"
     CDS             complement(32731..34356)
                     /gene="yhbX"
                     /locus_tag="ACHKW0_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7473417.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQKTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENDIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34813..34899)
                     /locus_tag="ACHKW0_000531"
     tRNA            complement(34813..34899)
                     /locus_tag="ACHKW0_000531"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34863..34865),aa:Leu,seq:gag)
     gene            complement(34914..35246)
                     /gene="secG"
                     /locus_tag="ACHKW0_000532"
     CDS             complement(34914..35246)
                     /gene="secG"
                     /locus_tag="ACHKW0_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HFO7473418.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35474..36811)
                     /gene="glmM"
                     /locus_tag="ACHKW0_000533"
     CDS             complement(35474..36811)
                     /gene="glmM"
                     /locus_tag="ACHKW0_000533"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HFO7473419.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36804..37652)
                     /gene="folP"
                     /locus_tag="ACHKW0_000534"
     CDS             complement(36804..37652)
                     /gene="folP"
                     /locus_tag="ACHKW0_000534"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HFO7473420.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVVPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37742..39676)
                     /gene="ftsH"
                     /locus_tag="ACHKW0_000535"
     CDS             complement(37742..39676)
                     /gene="ftsH"
                     /locus_tag="ACHKW0_000535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HFO7473421.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39776..40405)
                     /gene="rlmE"
                     /locus_tag="ACHKW0_000536"
     CDS             complement(39776..40405)
                     /gene="rlmE"
                     /locus_tag="ACHKW0_000536"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HFO7473422.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40531..40824
                     /gene="yhbY"
                     /locus_tag="ACHKW0_000537"
     CDS             40531..40824
                     /gene="yhbY"
                     /locus_tag="ACHKW0_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HFO7473423.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40980..41456)
                     /gene="greA"
                     /locus_tag="ACHKW0_000538"
     CDS             complement(40980..41456)
                     /gene="greA"
                     /locus_tag="ACHKW0_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HFO7473424.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41704..43137
                     /gene="dacB"
                     /locus_tag="ACHKW0_000539"
     CDS             41704..43137
                     /gene="dacB"
                     /locus_tag="ACHKW0_000539"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7473425.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43177..44349)
                     /gene="cgtA"
                     /locus_tag="ACHKW0_000540"
     CDS             complement(43177..44349)
                     /gene="cgtA"
                     /locus_tag="ACHKW0_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HFO7473426.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44365..45330)
                     /gene="yhbE"
                     /locus_tag="ACHKW0_000541"
                     /pseudo
     CDS             complement(44365..45330)
                     /gene="yhbE"
                     /locus_tag="ACHKW0_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
     gene            complement(45457..45714)
                     /gene="rpmA"
                     /locus_tag="ACHKW0_000542"
     CDS             complement(45457..45714)
                     /gene="rpmA"
                     /locus_tag="ACHKW0_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HFO7473427.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45735..46046)
                     /gene="rplU"
                     /locus_tag="ACHKW0_000543"
     CDS             complement(45735..46046)
                     /gene="rplU"
                     /locus_tag="ACHKW0_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HFO7473428.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46305..47276
                     /gene="ispB"
                     /locus_tag="ACHKW0_000544"
     CDS             46305..47276
                     /gene="ispB"
                     /locus_tag="ACHKW0_000544"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HFO7473429.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVQEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47504..47782
                     /gene="sfsB"
                     /locus_tag="ACHKW0_000545"
     CDS             47504..47782
                     /gene="sfsB"
                     /locus_tag="ACHKW0_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HFO7473430.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47830..49089)
                     /gene="murA"
                     /locus_tag="ACHKW0_000546"
     CDS             complement(47830..49089)
                     /gene="murA"
                     /locus_tag="ACHKW0_000546"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HFO7473431.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49144..49398)
                     /gene="ibaG"
                     /locus_tag="ACHKW0_000547"
     CDS             complement(49144..49398)
                     /gene="ibaG"
                     /locus_tag="ACHKW0_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HFO7473432.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49558..49851)
                     /gene="mlaB"
                     /locus_tag="ACHKW0_000548"
     CDS             complement(49558..49851)
                     /gene="mlaB"
                     /locus_tag="ACHKW0_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HFO7473433.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49851..50486)
                     /gene="mlaC"
                     /locus_tag="ACHKW0_000549"
     CDS             complement(49851..50486)
                     /gene="mlaC"
                     /locus_tag="ACHKW0_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HFO7473434.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50505..51056)
                     /gene="mlaD"
                     /locus_tag="ACHKW0_000550"
     CDS             complement(50505..51056)
                     /gene="mlaD"
                     /locus_tag="ACHKW0_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HFO7473435.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51061..51843)
                     /gene="mlaE"
                     /locus_tag="ACHKW0_000551"
     CDS             complement(51061..51843)
                     /gene="mlaE"
                     /locus_tag="ACHKW0_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HFO7473436.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51851..52660)
                     /gene="mlaF"
                     /locus_tag="ACHKW0_000552"
     CDS             complement(51851..52660)
                     /gene="mlaF"
                     /locus_tag="ACHKW0_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HFO7473437.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52870..53847
                     /gene="yrbG"
                     /locus_tag="ACHKW0_000553"
     CDS             52870..53847
                     /gene="yrbG"
                     /locus_tag="ACHKW0_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HFO7473438.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53861..54847
                     /gene="kdsD"
                     /locus_tag="ACHKW0_000554"
     CDS             53861..54847
                     /gene="kdsD"
                     /locus_tag="ACHKW0_000554"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HFO7473439.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELEQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54868..55434
                     /gene="kdsC"
                     /locus_tag="ACHKW0_000555"
     CDS             54868..55434
                     /gene="kdsC"
                     /locus_tag="ACHKW0_000555"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HFO7473440.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55431..56006
                     /gene="lptC"
                     /locus_tag="ACHKW0_000556"
     CDS             55431..56006
                     /gene="lptC"
                     /locus_tag="ACHKW0_000556"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HFO7473441.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55975..56532
                     /gene="lptA"
                     /locus_tag="ACHKW0_000557"
     CDS             55975..56532
                     /gene="lptA"
                     /locus_tag="ACHKW0_000557"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HFO7473442.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56539..57264
                     /gene="lptB"
                     /locus_tag="ACHKW0_000558"
     CDS             56539..57264
                     /gene="lptB"
                     /locus_tag="ACHKW0_000558"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HFO7473443.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57312..58745
                     /gene="rpoN"
                     /locus_tag="ACHKW0_000559"
     CDS             57312..58745
                     /gene="rpoN"
                     /locus_tag="ACHKW0_000559"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HFO7473444.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDDDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58768..59055
                     /gene="hpf"
                     /locus_tag="ACHKW0_000560"
     CDS             58768..59055
                     /gene="hpf"
                     /locus_tag="ACHKW0_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HFO7473445.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59173..59664
                     /gene="ptsN"
                     /locus_tag="ACHKW0_000561"
     CDS             59173..59664
                     /gene="ptsN"
                     /locus_tag="ACHKW0_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HFO7473446.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLEAPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59710..60564
                     /gene="rapZ"
                     /locus_tag="ACHKW0_000562"
     CDS             59710..60564
                     /gene="rapZ"
                     /locus_tag="ACHKW0_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HFO7473447.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60561..60833
                     /gene="npr"
                     /locus_tag="ACHKW0_000563"
     CDS             60561..60833
                     /gene="npr"
                     /locus_tag="ACHKW0_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HFO7473448.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61047..61679
                     /gene="yrbL"
                     /locus_tag="ACHKW0_000564"
     CDS             61047..61679
                     /gene="yrbL"
                     /locus_tag="ACHKW0_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HFO7473449.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61676..62404)
                     /gene="mtgA"
                     /locus_tag="ACHKW0_000565"
     CDS             complement(61676..62404)
                     /gene="mtgA"
                     /locus_tag="ACHKW0_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HFO7473450.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62401..63054)
                     /gene="elbB"
                     /locus_tag="ACHKW0_000566"
     CDS             complement(62401..63054)
                     /gene="elbB"
                     /locus_tag="ACHKW0_000566"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HFO7473451.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63284..65620)
                     /gene="arcB"
                     /locus_tag="ACHKW0_000567"
     CDS             complement(63284..65620)
                     /gene="arcB"
                     /locus_tag="ACHKW0_000567"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HFO7473452.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLCFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65716..66645)
                     /gene="yhcC"
                     /locus_tag="ACHKW0_000568"
     CDS             complement(65716..66645)
                     /gene="yhcC"
                     /locus_tag="ACHKW0_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HFO7473453.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
     gene            67320..71780
                     /gene="gltB"
                     /locus_tag="ACHKW0_000569"
     CDS             67320..71780
                     /gene="gltB"
                     /locus_tag="ACHKW0_000569"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HFO7473454.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            71793..73211
                     /gene="gltD"
                     /locus_tag="ACHKW0_000570"
     CDS             71793..73211
                     /gene="gltD"
                     /locus_tag="ACHKW0_000570"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HFO7473455.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEDNMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            73395..74522
                     /gene="yhcG"
                     /locus_tag="ACHKW0_000571"
     CDS             73395..74522
                     /gene="yhcG"
                     /locus_tag="ACHKW0_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDDEXK nuclease domain-containing protein"
                     /protein_id="HFO7473456.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(74582..75046)
                     /gene="nanQ"
                     /locus_tag="ACHKW0_000572"
     CDS             complement(74582..75046)
                     /gene="nanQ"
                     /locus_tag="ACHKW0_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate anomerase"
                     /protein_id="HFO7473457.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(75043..75918)
                     /gene="nanK"
                     /locus_tag="ACHKW0_000573"
     CDS             complement(75043..75918)
                     /gene="nanK"
                     /locus_tag="ACHKW0_000573"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HFO7473458.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQWLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(75915..76604)
                     /gene="nanE"
                     /locus_tag="ACHKW0_000574"
     CDS             complement(75915..76604)
                     /gene="nanE"
                     /locus_tag="ACHKW0_000574"
                     /EC_number="5.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009385 - N-acylmannosamine-6-phosphate
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006051 - N-acetylmannosamine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019262 - N-acetylneuraminate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HFO7473459.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(76652..78142)
                     /gene="nanT"
                     /locus_tag="ACHKW0_000575"
     CDS             complement(76652..78142)
                     /gene="nanT"
                     /locus_tag="ACHKW0_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HFO7473460.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(78251..79144)
                     /gene="nanA"
                     /locus_tag="ACHKW0_000576"
     CDS             complement(78251..79144)
                     /gene="nanA"
                     /locus_tag="ACHKW0_000576"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008747 - N-acetylneuraminate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HFO7473461.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(79266..80057)
                     /gene="nanR"
                     /locus_tag="ACHKW0_000577"
     CDS             complement(79266..80057)
                     /gene="nanR"
                     /locus_tag="ACHKW0_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HFO7473462.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT        19769 a        21656 c        19889 g        18875 t
ORIGIN      
        1 gaaatatttc tacctgtata atctttaaag atgttgaaca tatattcaca ttaaatatga
       61 ttatgtactt gttacaagga taaggttata aatgaataaa gttacaaaaa cagctattgt
      121 gggcttactt gcgcttttcg caggtaatgc cgctgcaacc gatggtgaaa tagtttttga
      181 tggcgagatt ttaaaatccg cttgtgaaat caatgactct gataagaaaa ttgaagttgc
      241 tcttggtcac tataatgccg aacagtttcg ttctgttggc gatcgtagcc caaaaattcc
      301 attcactatt cctttagtta actgcccagt gactgggtgg gagcacgata acggcaacgt
      361 tgaagcgtct ttccgtttat ggctggaaac gcgggataat ggcaccgtcc ctaacttccc
      421 taatctggca aaagtgggct cttttgctgg cacagcggca acaggtgtgg gtatccgtat
      481 tgacgatgcg gaaagcggaa accttatgcc actgaatgct atggggaatg ataacacggt
      541 ttatcagatc ccagcggact ccaatggtat tgtcaatgtt gacctcatcg cttactacgt
      601 atcaaccgtt gaagcatcag aaatcacccc aggggaagca gacgctgtcg ttaacgtaac
      661 gctggattac cgttaatacg ttacggcgtt atctgacctt tcagataacg cccttttcct
      721 tcctctttct cgttgtatca ggttgaaaaa taactatgtc aaaacgaaca ttcgcggtga
      781 taataacctt gttgtgtagc ttctgtatcg gccaggcgct tgcagggggt atcgttttac
      841 agcgaacgcg agtgatctat gatgccagcc gcaaagaggc tgcgttacct gtcgcaaaca
      901 aaggcgcaga aacgccttat ttactgcaat catgggtaga taatatagat ggtacaagcc
      961 gtgccccatt tattataacc ccaccgctat ttcgtcttga agctggcgat gactcatcac
     1021 tgcgaattat taaaacagct gataacctgc ctgaaaataa agagtcgctg ttctacatta
     1081 atgttcgtgc cattccagca aagaaaaaat cagatgatgt taatgctaac gagttgacgc
     1141 tggtatttaa aacacggatc aaaatgtttt atcgccccgc acacctgaag ggacgggtaa
     1201 acgatgcgtg gacatcactg gaatttaaac gtagtgacca ttcactcaat atatataacc
     1261 caactgaata ttacgtcgta tttgccggac tggcagtcga taaaaccgat ctcacaagca
     1321 aaattgaata tatcgcgccc ggagaacata aacagttacc acttcctgca tctggcggaa
     1381 agaacgtgaa atgggctgca atcaatgatt atggcggcag ttccgggaca gaaactcgtc
     1441 cactgcaata acaaatataa aaaacacagg tcatcaggga atgccacaac gacaccacca
     1501 gggacataaa cgcacaccga aacagttggc gctcatcatc aaacgctgtt tgccgatggt
     1561 gctcactggc agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc
     1621 cattatgctg gaaactacaa aaagtggtat gcaaacaact gatctgtcac gtttttcaaa
     1681 aaaatacgca caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt
     1741 ttcacagaaa aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac
     1801 ggtagaacaa ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa
     1861 agatgacgat agcgtgatca actcgcttga acaaatcatt cccggtacag ctgctgaatt
     1921 tgatttcaat catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc
     1981 aagaggttac gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta
     2041 ctcgtttaca ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct
     2101 gaatatgcaa aatggtgcta attttggccc ctggcgatta cgtaactatt ctacgtggac
     2161 acgcaacgat caggcgtcaa gctggaacac tatcagtagt tatttacaac gtgatatcaa
     2221 ggcgttgaag tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag
     2281 ctacaccttt actggcgtgc aactcgcttc cgacgataat atgttgccaa acagccagcg
     2341 cggatttgcc ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca
     2401 aaatggttat gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct
     2461 ctacccctct tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacgca
     2521 acgtcgcttt atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa
     2581 atatagcgcg accgctggac gctatcgcgc tgatgcaaac agtgatagca aggaacccga
     2641 atttgctgaa gccacggcaa tatatggttt gaataatact tttacgctgt atggcggcct
     2701 gctcggttct gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact
     2761 gggcgcgttg tcgatggata tcaacagagc tgacacccaa ttcgataacc agcactcttt
     2821 tcatggctat caatggcgta cgcagtacat caaagatatc ccggaaacca acaccaatat
     2881 cgctgtcagc tactatcgct ataccaacga tggctatttt agttttaatg aagccaatac
     2941 ccgtaattgg aactataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac
     3001 aatatttgat ggggtaagtc tgtatgcctc cggttcgcag caagactatt ggggcaataa
     3061 cgagaaaaac aggaatatct ctgttggggt ttccggtcag caatggggaa ttggttacag
     3121 cctgaattat caatacagtc gctacacgga tcaaaataat gaccgcgcac tctatttgaa
     3181 tctcagtatt ccgttagaac gctggttacc gcgtagccgg gtttcctatc agatgaccag
     3241 ccagaaagat cgcccaaccc aacatgaaat gcgtcttgat ggctcactgc tggatgatgg
     3301 tcgcctgagc tatagcctgg aacaaagtct ggatgacgat aacaaccata acagtagcct
     3361 gaacgccagt taccgttcac cttatggcac cttcagtgcc ggatacagct acggtaatga
     3421 cagcagccaa tacaattacg gcgttaccgg cggcgtggtt atccatcctc atggcgtgac
     3481 gctctcgcaa tatctgggca acgcttttgc gcttatcgat gctaatgggg cttctggcgt
     3541 gaggatacaa aactatccgg ggattgctac tgatcccttt ggctatgcag tggttcctta
     3601 tctcacgact taccaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt
     3661 cgatcttgag caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg
     3721 tttcaacgcc aatatcggtt atcgcgtact tgttacagtc agcgatcgca acggtaaacc
     3781 gttgcccttt ggcgctcttg ccagcaacga tgagacgggg caacaaagta tcgtcgatga
     3841 gggcggcata ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg
     3901 gggaaatcag gcagatcaac aatgtcagtt tgcttttagt acaccggatt cagaaccaac
     3961 aacctctgta ttacaaggca cggcgcagtg ccattaagga taaaaaaatg aaaagagcgc
     4021 ctcttataac aggacttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagggt
     4081 gtggagctga cagcactagc ggtgcgacaa attacagcag tgtggttgat gatgttacgg
     4141 tgaaccagac agataacgtg acaggacggg agtttacctc tgccacgcta agtagcacta
     4201 actggcaata cgcctgtacc tgctctgcgg gtaaggcagt taaacttgtc tatatggtca
     4261 gccctgtact taccaccact ggacatcaag caggatatta caaactcaat gatagtctgg
     4321 atattaaaac cacattacag gcgaatgaca ttccaggact cgtgactgac cagaccgttt
     4381 ctgttaacac ccgattcaca cagataaaaa gcaacaccgt atattctgct gcaacccaaa
     4441 cgggtgtttg ccagggtgac acgtctcgtt atggacccgt taatattggt gcaaatacca
     4501 cctttaccct gtatgtcacc aagccatttc tcggctcgat gaccattccg aaaacggata
     4561 ttgccgtcat taaaggcgca tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc
     4621 atgatttagt caagttatcg attcagggaa atctcaccgc cccacagtcg tgcaaaatta
     4681 atcagggcga tgttattaag gttaattttg gattcatcaa tggtcagaag tttaccaccc
     4741 gcaatgccat gccagatggt tttactccag tagactttga tatcacttat gactgtggtg
     4801 atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag
     4861 accagtacaa cctggtcgcc agacgaagaa gttcagacaa tgcgcccgat gtcggtattc
     4921 gtattgaaaa tctcggcggc ggagttgcaa atattccttt tcagaacggt atccttcccg
     4981 ttgatccttc cgggcatggc accatcaaca tgcgcgcctg gccagttaat ctggtcggtg
     5041 gtgagctgga aacaggaaaa tttcagggca ccgccaccat taccgtcatc gtgcggtaaa
     5101 agaagacgtt gcgttttgat taacgcaacg cttcggcact cttacccctg ctgctccagc
     5161 gcatacttat acagcgcatt tttcttcacg ccgtgaattt ctgccgccag cgccgccgct
     5221 tttttcaacg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag
     5281 tcttcttcct gtgctttatg accttcgata atcagcacca tttcgccttt gcgacggttt
     5341 tcatcttctt ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag
     5401 gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata
     5461 tcttccaggc tatctaacag acggtgggtg gattcataaa aaatcagcgt gcgcggctcc
     5521 gcttcaatgg cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttcg
     5581 taacagaaac ggtcagaggg taaacccgcg gcgcttaacg cagtgatagc agcacacggc
     5641 ccgggtagcg gcaccacgca aatccccgct tcacggcagg tacgcaccag atggtagcca
     5701 ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttctg cccttcttgc
     5761 agcttcgcca gcagcgtttc ggctttttgt tgttcgttat ggtcgtgcag cgcaaacaac
     5821 cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca
     5881 atcagatcaa cggcctgtaa tacctctaac gcacgctggg tgatatccgc cagattgccg
     5941 attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc
     6001 attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaat atcactggat
     6061 acattatggt accctcaaca ttttctcgtt tgaaagccgc gcgttgtctg cctgttgttc
     6121 tggcagccct gattttcgcc ggttgtggca cccatactcc cgatcagtcc actgcttata
     6181 tgcagggcac gacgcaggct gattctgcct tttatcttca gcagatgcag caaagctctg
     6241 atgataccag gatcaactgg caattactcg ccattcgtgc actggtgaaa gaaggtaaaa
     6301 ccgggcaggc ggttgagttg tttaaccaac taccgcaaga actgaacgat tctcagcgtc
     6361 gcgagaaaac actgctggcg gtagagatta aactggcgca gaaagatttt gctggcgcgc
     6421 aaaacttgct ggcgaaaatc acacctgccg atttagaaca aaaccagcaa gcgcgttact
     6481 ggcaggcaaa aatcgatgcc agccaggggc gtccttccat tgatttactg cgcgcgttaa
     6541 ttgctcagga accgctgctc ggcgcgaaag aaaaacagca gaatattgat gccacctggc
     6601 aggcgctctc ctccatgact caggaacagg cgaatacgct ggtgatcaac gccgacgaaa
     6661 atattctgca aggctggctg gatctgcagc gcgtctggtt tgataaccgt aacgatcccg
     6721 acatgatgaa agccgggatc gccgactggc aaaaacgtta tccgaacaat ccgggcgcga
     6781 aaatgctgcc aacgcagttg gttaacgtaa aagcgtttaa accagcctcg accaacaaaa
     6841 tcgccctgct gttgccgctg aatggccagg cagcggtatt tggtcgcact attcagcaag
     6901 gctttgaagc ggcgaaaaat atcggcactc agccagtggc ggctcaggta gctgccgcac
     6961 ctgccgcaga cgtagctgaa caacctcagc cgcaaaccgt ggatggcgtt gccagcccgg
     7021 cacaagcctc ggttagcgat ctgaccggtg aacagcctgc ggcacagcca gcgcctgtaa
     7081 gcaccccggc agcaaccacc acagcggtaa gcgcgccagc gaatccatcc gcagaactga
     7141 aaatctacga tacctcatca caaccactta gccagatctt aagccaggtt cagcaggatg
     7201 gcgcgagtat tgtggttggt ccgttgctga aaaataacgt tgaagagttg cttaagagca
     7261 acaccccgct gaacgtgctg gcactgaacc agccggagaa tatcgaaaac cgcgtcaata
     7321 tttgttactt cgcgctttca ccggaagacg aagcgcgcga tgcagcgcgt catattcgtg
     7381 accagggtaa acaagcgccg ctggtgctga tcccacgcag ttcattgggc gatcgcgtag
     7441 ccaatgcgtt tgcgcaggag tggcagaaac tgggcggcgg caccgttctg caacaaaaat
     7501 ttggttccac cagcgaatta cgcgcgggtg ttaacggcgg ttctggtatc gctttaacgg
     7561 gtaccccgat tactcccaga gcgacaaccg atgaccagtt caccaataat ggcggtcgtg
     7621 tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa ccgatgatcg
     7681 ccatgcgtaa cggtagccag agcggtgcaa cgctgtacgc cagctcccgc agtgcgcaag
     7741 ggaccgctgg cccggatttc cgactggaga tggaaggctt gcagtacagc gaaatcccga
     7801 tgctggcagg cggtaatcta ccgttaatgc agcaggcact cagcgcggtg aataacgatt
     7861 attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca aatcatttct
     7921 cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc ctgacggcaa
     7981 acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa ggtcaggtag
     8041 tccccgccag ttaaccacca aacagaccgg cgatgcgtgg gaagtacaag cgcgtcgctg
     8101 gctggaaggc aaaggactgc gatttgtcgc cgctaacgtg aacgagcgtg gcggcgagat
     8161 cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct atcgccgctc
     8221 tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca aattattaca
     8281 gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg attgccggtt
     8341 cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct ttaatgacca
     8401 ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc ttcactgaaa
     8461 gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc cgtgcagcca
     8521 tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt aatggaactt
     8581 ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa acggagcggc
     8641 ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg attgccaacg
     8701 atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat gcgggagatg
     8761 tattgttagc catttccacc cgtggcaaca gccgcgatat tgtaaaagcg gtagaagctg
     8821 ccgttacgcg tgatatgact attgtggcat tgaccggcta tgacggcggc gaacttgcag
     8881 gtttgttagg gccacaagat gtggagatcc gcattccttc gcatcgtagt gctcgcattc
     8941 aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac acgcttttcc
     9001 ctcaccagga tgtttaagga gaatacatga aggcattatc gccaatcgca gtccttattt
     9061 ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct gctgtgggta
     9121 ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt accctggaag
     9181 tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaagcc cgtattaatg
     9241 tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct gaactttcgg
     9301 ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat aacgagattc
     9361 gtcagggcca gccgattggt ctgggcgaag cgtctaacga tacgtggatc accaccaaag
     9421 tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa gtgaccaccg
     9481 aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa gcggcggcag
     9541 atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg tttattaaat
     9601 agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc tacaacgatg
     9661 caaactgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc tcaaatcaga
     9721 acaacagcgc cagcccgccg acaatcacac cagacactgc caccatcgcc cctgtcagcc
     9781 ataaggcttt cgctgggaac gctttgcgca gcataatcag tgacggcaaa ctcaccgccg
     9841 ggagcgtcat caacaatgcc agcgccggag cagttcccat acctgccagc atcatcgttt
     9901 gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct accgccatcg
     9961 ccatcaccca catcaggctg ttatcgacag caccatcggc atgggggaat aaccagacgc
    10021 gagcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc cagaaaagcg
    10081 tccatagcgc cctgccccag cggctaaaaa aaccgccctg tgcttccggt atgtcaattt
    10141 cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc gtcgcaatca
    10201 gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag ccgaggacaa
    10261 accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag aatgccagcg
    10321 caccgcccat cgacacctgt tggcgacgca ttcccgccgc gaccggagcc gcacagcagg
    10381 tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga aagcgcgatt
    10441 gcccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac gaaccgagga
    10501 tcacccccag cactgccgct ttccataccg cgaggaaata gatcatcgcg taatccaacg
    10561 ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata ctgtgggttt
    10621 cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag agaccggcaa
    10681 tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc gcctgagatg
    10741 aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc gcgtgaatat
    10801 tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat tctgaagagc
    10861 ttaaaatcgt cacaccttca tgcccgggcc gcatcgcctc tgccagcatg acccgcgcca
    10921 cgtctctcgc atcaatggat ttccagttac cgggtaataa acggaacagc ggcgcaaaaa
    10981 gcgtttcgtt catccgctgt ttgctacgat cgcccagtaa catcgacggg cgagcaatgg
    11041 tcaatttcgg ccagttctgg gcgattaatg cttcttccat ctcccctttg acgcggttat
    11101 agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata tgctgcgcgc
    11161 ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca tgaataaacg
    11221 cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca atgtcgatgg
    11281 gatcggtgac ctgcgccagc gcgtcagtca gttgcggatc atggggatta aacaccccgg
    11341 gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc ggttcgttaa
    11401 tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc agtacctgac
    11461 tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg caccaggctt
    11521 aatagaccat gaggtaatta tccccgattg tgggaaattc gcctcatcca atgcaacaac
    11581 gcggaggaag catgagtaag aaaattgccg ttttaatcac tgatgaattt gaagattcag
    11641 aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt accattgaaa
    11701 aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc atcgataaat
    11761 ccatagatga agtgacgcca gcggagtttg atgccctgct gctaccgggc ggccattcac
    11821 cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt gtgaatagcg
    11881 gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc gatgtgattc
    11941 gggggcgcaa actgaccgcg gttaaaccga tcattattga tgttaaaaac gcgggcgcgg
    12001 aattttacga tcaggaagtc gtggtcgata aagatcagct ggttaccagc cggacaccgg
    12061 acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga gccgcgtagc
    12121 caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc atcgagccgg
    12181 atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt gtacgccttg
    12241 cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa ctgcacgccg
    12301 cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc agcctgcggc
    12361 ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc gactttctgc
    12421 gcatcaaaca gataaaaagc attagcgcac cagagttccc cttcctgctg cacacaccag
    12481 gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc cattgctgtt
    12541 ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac cctggtttct
    12601 ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg atgtcgaacg
    12661 ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga aaggagaact
    12721 gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg cggaatatcg
    12781 cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaagcg cagggtttgc
    12841 agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg ctcgtgatac
    12901 tcaaccagac cagttacgcc attcaacgcg tcatccgcta aacgatgtac ctggaaggcg
    12961 ctttcagtgc ctggccagcg gcaacgcagg tcgtgatgtg ccgccagttc aaagccaaaa
    13021 cggctgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa ctcattaagc
    13081 gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta tttttcatca
    13141 accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc aaccggacta
    13201 aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc cagcgtcaga
    13261 aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc gaatgagcga
    13321 cgtagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat tagcatggtt
    13381 cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca acaaaatccg
    13441 ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca atggcgtcca
    13501 tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc cgacgctgga
    13561 agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg ctggcgtcgg
    13621 cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca ttcaccaccg
    13681 aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg ccttcaagaa
    13741 actccagctc gccatcatcc agcgcctgac ggaattgcca gcttaagacc tgctcaagaa
    13801 cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga gatggcccca
    13861 aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt ttcaacaatc
    13921 atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat tccatatgaa
    13981 attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc gcagcaaggt
    14041 taactaaaat cccagttcgt taacattttt gcgttttgat agcgcaacct tcaggaaaaa
    14101 ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc atcgaaaacg
    14161 gcgcagatgc tgtttatatc gggctaaaag atgataccaa tgcccgtcac ttcgccggcc
    14221 ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa catcgccgca
    14281 aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt tggcagcgtg
    14341 ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac ctcgccatgc
    14401 tagagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg caggcttcgg
    14461 cgaccaatga agaggcgatt aacttttatc atcgccattt tgacgttgct cgcgtggtgc
    14521 tgccgcgcgt gttgtcgatt catcaggtga aacaactggc acgggtcaca cctgtaccac
    14581 tggaagtctt tgctttcggc agcctgtgca ttatgtcgga aggtcgttgc tatctgtcgt
    14641 cgtatctgac gggtgagtcg cccaacaccg taggcgcgtg ttctccggcc cgtttcgtgc
    14701 gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg atcgaccgtt
    14761 atcaggacgg cgaaaacgca ggttatccga cgctatgtaa agggcgttat ctggtggacg
    14821 gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa ctgctgccgg
    14881 agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt agcccggcgt
    14941 atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag gccgatccgc
    15001 aaaacttcat accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc gaaggcacgc
    15061 agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga aatattcctt
    15121 agggccagtg ctgtggtact ggccaaaaga gacgctggaa gatttttatc agcaggccgc
    15181 caccagcagc gccgacgtga tttatcttgg tgaagcgata tgcagcaagc gccgggcaac
    15241 caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagtggca agcagattgt
    15301 gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga aacgctatgt
    15361 tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata tgtgcgctga
    15421 acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg tgacgctgaa
    15481 aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt cccgcgactg
    15541 gctggtgaat ctgcttaatc agtgcgatga gctgggcatt cgcaaccagt ttgaagtgga
    15601 agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta ccgcgcgttc
    15661 ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga acgggcgcaa
    15721 cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga ccatgagcgg
    15781 ctacgtttac aacctcggta acgagctggc atccatgcag gggctggttg atgtggtccg
    15841 cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg ctaatgaaaa
    15901 tggcgctgcg ccactaccgt tgacggcaaa cagcgactgt aacggctact ggcggcggct
    15961 ggccgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag ttgcatgtgc
    16021 tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg attcactctc
    16081 ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta acaactttaa
    16141 ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt atgactgata
    16201 aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct tcagcgcgag
    16261 aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc tatcatcgct
    16321 actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg tcggtattga
    16381 ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc gtgatgttgc
    16441 ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca ctctatccgg
    16501 ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca atgatggcgc
    16561 tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg gagctggtgg
    16621 actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc tatggcgaga
    16681 aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg gcgcagcttg
    16741 gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag gcgctgcatc
    16801 tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg atggtgtgca
    16861 tcaatattat cgccgccgat agcaaccgcg acgctgagtt tctgtttacc tcaatgcagc
    16921 aagcctttgt gaagttgcgc cgtggcgaaa ccgggcaact gccgccaccg attcaaaata
    16981 tggatcagtt ctggtcgccg tccgaacagt atggtgtgca gcaggcgctg agtatgtcgt
    17041 tggtaggtga taaagcgaaa gtgcgtcatg gcttgcagtc gatcctgcgc gaaaccgacg
    17101 ccgatgagat tatggtcaac gggcagattt tcgaccacca ggcgcggctg cattcgtttg
    17161 agctggcgat ggatgttaag gaagagttgt tgggatagtg tgtcttaacg cgggaagcct
    17221 tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat caggcattgt
    17281 gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg cagtaaatta
    17341 aagctcgata aaatatggac cagtgcgttg ccaacgccaa acaccagaat cagcgtaatc
    17401 atcggcttgc cgccccagac gcggaatttc gggctgccaa agcgtttacg cgatgcgcgg
    17461 gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc ataaccaatg
    17521 gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc aaaggtcagc
    17581 aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc tgccagatag
    17641 tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt tgagaacacg
    17701 accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg taccagcaca
    17761 tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat gttacccatc
    17821 gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta caccaggcat
    17881 ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggcttggcac gttaccgtga
    17941 taaccaaacg acgccaggca gaacggcaat gtcatcagca gatacggcgc ataagatgca
    18001 ttgctttctg caacgttgaa caatgtcgca ggctgcacat gccccaacag gctgccaaag
    18061 gtgaggaaga aggtaatgac tttcgccccc agcacaatcg ccgtcatgcg gctgacggcc
    18121 ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc cgccgcccgt
    18181 gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc actggcagaa
    18241 atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt gaccacgttc
    18301 cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat tcggtaattc
    18361 aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt aaagatcagc
    18421 gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga aaacatccct
    18481 gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg tgacgtttgg
    18541 gtggtggtta gtgttgccat gagggcttct ctccagtgaa aaatagtgcg actgcgttgt
    18601 tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc ccgatggtaa
    18661 aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga tgattacgca
    18721 tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg gccttcacga
    18781 cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc accactgaag
    18841 ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg acgctcaccg
    18901 ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt gagaatgcga
    18961 gtacgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc gatggtggag
    19021 tgagaagcaa acagcttgat gttaccaatg taacggctgc tgatgtcgcc ttcgttagca
    19081 atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc aatgcggtac
    19141 agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg gtcaccacgc
    19201 gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa ttcacgtttc
    19261 ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc cattttcagc
    19321 agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg ctgaattttg
    19381 ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg tactttagcg
    19441 gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc tacttccgga
    19501 atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg gttctcaacg
    19561 aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg gtgaacgtaa
    19621 gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc aacgtccagg
    19681 ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt caggcgttcc
    19741 agtgtctgtt cacgaagcgc ctggttcatg tcaccgttca gcgcggcgct gttgtagccg
    19801 ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac gaagataatc
    19861 gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt gcgcataccc
    19921 cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga ctgaatgcgc
    19981 acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc cggcatggtt
    20041 gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt ttcaacgtct
    20101 tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc gctcagttta
    20161 gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt accgacaacg
    20221 atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc gccgtacaga
    20281 gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc agcaacctgt
    20341 accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag ctcaggatca
    20401 agattctgca gcagaggtaa agagaatgct gcagtttttc cgctccccgt ctgggccata
    20461 cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat tggagatggt
    20521 ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc cagatctgca
    20581 aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg cggccagtct
    20641 acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac cggctcaaag
    20701 taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat caaaaaagat
    20761 tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca tcttggtcct
    20821 ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta tgaacgttgt
    20881 tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga cttaggtagt
    20941 acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg ttatccgttg
    21001 cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg tagaactcga
    21061 caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc aacacttctt
    21121 tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta cggaaaggat
    21181 cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg tcacgaccgc
    21241 cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga tcaagctcaa
    21301 gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt aaatatatgc
    21361 ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc gaaaaatcgt
    21421 tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca tataaaagct
    21481 gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata cgtgccagaa
    21541 tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt ttacgccagg
    21601 aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag cgcaaaaaag
    21661 gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc ggttgctaac
    21721 aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg caaccttact
    21781 cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc tcagtcgctt
    21841 ctttaatgct cagacggata cggccctggc gatcaacttc cagaactttc accggtactt
    21901 cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg atttgagaga
    21961 tgtgtaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag tcaacgatac
    22021 gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc tcttcgatac
    22081 gacgaatagc gtgtttcgct ttctcgccgt cggtcgctgc gatcttcaca gtaccgtcat
    22141 cttcgatttc gatggtggtg ccagtttctt cggtcagggc acggataaca gaaccgcctt
    22201 taccgataac gtctttgatc ttgtccgggt tgatcttgat ggtatggata cgcggtgcga
    22261 actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc aggatatgca
    22321 gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg ataccttcaa
    22381 ttttgatatc catctgcaat gcagagatac cgtcgcggga acccgcaact ttgaagtcca
    22441 tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag ttgtcgcctt
    22501 ctttcaccag acccattgcg atacccgcaa cggcagcttt gatcggcaca cctgcgtcca
    22561 tcagcgccag agacgcgccg cacacggaag ccatagaaga ggaaccgttg gattcagtga
    22621 tttcagacac aacacgtacg gtgtacggga atttgtccat atccggcatg actgccagca
    22681 cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa ccgaccatgc
    22741 cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggtg tcggtacgtt
    22801 cgcccatcag ttcatcaaga acctgcgcgt cacgagcagt acccagcgtt gcggtaacca
    22861 gcgcctgcgt ttcaccacgg gtgaacagcg cagaaccgtg agtacgcggc agcacgccag
    22921 tacgcacatc cagaccacga atcatatctt tttcacgacc gtcgatacgc ggttcgcctg
    22981 ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca cccagttcgt
    23041 tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat ttgatgacat
    23101 caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc agacgagctt
    23161 cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc tgccagtccc
    23221 aacgtggttt accggcttct ttcaccagtt cattgatgtt ctgaataaca acctgctgtt
    23281 gttcatgacc gaacactact gcgcccagca tctggtcttc gctcagcaat tcagcttcag
    23341 attcaaccat cagtacagcg gcttcagtac cagcaacaac cagatccagt ttgctctctt
    23401 tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct acgcgggcag
    23461 caccaatcgg accattgaac ggaataccag acagagacag tgctgcggaa gcaccaatca
    23521 tcgcgacgat atccggatta acttgcgggt taacagaaac cacggtggcg ataacctgaa
    23581 cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc agacgcgcga
    23641 tcagggtttc gccttcgctt gggcggcctt cacgacggaa gaagctaccc gggatacgac
    23701 cagcagcgta agtacgctcc tgatagttaa cggtcagtgg gaagaagtcc tgacctggtt
    23761 tggctttttt ctggccaaca acggtaacga ataccgcggt gtcatccatg ctaaccataa
    23821 cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg tgttggccgt
    23881 actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt cttagacagt
    23941 accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact aatgacaacc
    24001 ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct gcaacggaag
    24061 atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc ttgaaaaaag
    24121 gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga cccaggcgct
    24181 cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc agtttacgac
    24241 gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg tgctctgcaa
    24301 agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact tcggtagaac
    24361 cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct tcagtactta
    24421 gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct aattcagcga
    24481 tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg gcagtattct
    24541 actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc aaccaccagg
    24601 cgacgaggcg caacgcggcc ttcatcgtca atttcgccca taccgataaa tttgccgttc
    24661 tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg aactgggtta
    24721 ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta gtccgaagct
    24781 ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat atcctgctgt
    24841 tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc aaccggatat
    24901 ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag tttttcaccc
    24961 aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc cagctccagc
    25021 tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg cgcttcacgc
    25081 ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata tttcagtgct
    25141 gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc cgccgccagt
    25201 tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc atcagaagta
    25261 tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc cagcagatac
    25321 tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgccagttgc cagcgggtcc
    25381 agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt ttgcagcgca
    25441 tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat gtcgcgaccg
    25501 cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca cgacgttctt
    25561 cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca accagagagt
    25621 tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc cccagcaggc
    25681 tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc gcgtcttcat
    25741 ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga gacatttcga
    25801 caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc tgcaggatga
    25861 gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat tctttcgcca
    25921 taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta atttcaggtg
    25981 gaagggctgt tcacgttggc ctgataagac gcgccagcgt cgcatcaggc agtccatgca
    26041 ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc gatggtacgt
    26101 tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc gttgtagttc
    26161 ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc tttgaagcgg
    26221 cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac gcggatcggg
    26281 ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatggcacc aaatttcggt
    26341 gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag ttccggagac
    26401 agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac ggagtagtaa
    26461 cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc acgtacgttg
    26521 aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc ggtgataccc
    26581 cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt cagcaaggag
    26641 tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatatt cacttcgtga
    26701 acttcgcctt cggtcatgtt ggcaaacatg ttctcgagtt tagatttttg ctgacgcgcc
    26761 agtttaactt cgcggaattt accctgacga tagagtgcaa cttcacgcgc tttcttctcg
    26821 tcacgtacaa cggtaacttc atcacccgca gccggaacgc cggacaggcc gaggatttcc
    26881 accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg catcgcacga
    26941 acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt accttcacgt
    27001 accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat aaccgcaccg
    27061 ctcgccatac ctttacgtac cgcttttagc tccagaactt ccgcctgcag caggatagcg
    27121 tccagcagtt catcgatacc ggtacccgcc ttcgcagata cgtgtacgaa ctggctttca
    27181 ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac acgatccgga
    27241 tcggcttctg gtttatcgat cttgttcact gcaaccacca ccggtacctg cgccgctttc
    27301 gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc aacaaccagg
    27361 actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc agcgtgcccc
    27421 ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc accaatgtgc
    27481 tgggtaatgc cgcccgcttc gccagaggcc actttcgttg aacgaatgta gtccagcaga
    27541 gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg cggttcagcc
    27601 gcagcccccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc acgacgcagg
    27661 ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc gataacctgg
    27721 ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga gcctttaacc
    27781 gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac gtcacggtta
    27841 acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg ttttccgcct
    27901 ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc agcgtgtttg
    27961 ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacggcc gccttcgact
    28021 tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagttgtgac atgataatcg
    28081 ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc catacgacgt
    28141 gcttcttcag caacgcgacg tgcttcttct tcgagtttac gacgcgcttc ttcttcagct
    28201 ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg ggcgttttta
    28261 gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg tttcgcttgt
    28321 tcagcagctt cacgcttagc ttgttctgcg gcctcacgtt cagctttttg ttgcgcctcg
    28381 cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg ctgcgcttgc
    28441 tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt gcgtttcttg
    28501 cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt aagggtgctg
    28561 cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt caggtggtca
    28621 atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg gatacctgca
    28681 tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc cagcgtttta
    28741 atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc gtcaccgaac
    28801 cagcaaatat tacgggcagc cataatcagt gctccggctt tttcgtcggt caacccttcg
    28861 atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca aacgccacgg
    28921 gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag atcgtcagcc
    28981 ggtttgttat caccgaggct ttcttccagg gcctgtgcaa tggtggccag tgcattttta
    29041 gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc caacagctct
    29101 ttcatcggaa cataggccag ttcttccagc gtcgagaaac cttcttctac cagaacagtc
    29161 gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc
    29221 gcctgatgct tagcctgcag gtcgtcaacg gtcattacgt tgagttccca accgctcagc
    29281 tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag gttaccggct
    29341 tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc aacgtctgcc
    29401 ggtgccattg cgttaatcac aaactgcgcc gggttatcat cccacaggac gatatcgata
    29461 cgctcgccgc ccagttcagt agaaaccgcc tgaacacgcg cgccacgcat acctacacag
    29521 gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc acgagaaccc
    29581 ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg cacttcaata
    29641 cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg cgcgccacgc
    29701 gcttccgggc gaacggaata gagcacgcca cgaacgcggt cgccagggcg gaagttttca
    29761 cgcggcagca tatcttcgcg caggatcacg gcttcagcat tgttgccgag atccaaagag
    29821 atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc gtgttcacgg
    29881 aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat aacctgtttt
    29941 gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc aacgtaatcg
    30001 cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc cttggtcggc
    30061 tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc gcttttgcga
    30121 tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc tgtcgccagc
    30181 gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt ggatacggct
    30241 tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc agactattaa
    30301 aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc atctttacct
    30361 tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg ccatttacga
    30421 cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa acgggcgtag
    30481 tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag gttataagca
    30541 acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac atcagcacaa
    30601 tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga tgtgcgaccg
    30661 cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc agtaatcatc
    30721 tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa aaagggctta
    30781 aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg ctttatataa
    30841 ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt tttcaaatcc
    30901 cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg gaaagtggtt
    30961 gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc taccaggctg
    31021 ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaag atgcaaatac
    31081 tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata gtgatccatg
    31141 ccacattttg tcaacgttta ttgctaatca cgtgaatgaa tatccaattc actttcattt
    31201 gttgaatact tttgccttct cctgctctcc cttaagcgca ttattttaca aaaaacacac
    31261 taaactcttc ctgtctccga taaaagatga ttaaatgaaa actcatttat tttgcataaa
    31321 aattcagtga gagcggaaat ccaggctcat catcagttaa ttaagcaggg tgttatttta
    31381 tgacgacgat tctcaagcat ctcccggtag gtcaacgtat tggtatcgct ttttctggcg
    31441 gtctggacac cagtgccgca ctgctgtgga tgcgacaaaa gggagcggtt ccttatgcat
    31501 atactgcaaa cctgggccag ccagacgaag aggattatga tgcgatccct cgtcgtgcca
    31561 tggaatacgg cgcggagaac gcacgtctga tcgactgccg caaacaactg gtggccgaag
    31621 gtattgccgc tattcagtgt ggcgcatttc ataacaccac cggcggcctg acctatttca
    31681 acacgacgcc gctgggccgc gccgtgactg gtaccatgct ggttgctgcg atgaaagaag
    31741 atggcgtgaa tatctggggt gacggtagca cctataaagg aaacgatatc gaacgtttct
    31801 atcgttatgg tctgctgacc aatgctgaac tgcagattta caaaccgtgg cttgatactg
    31861 actttattga tgaactgggc ggccgtcatg agatgtctga atttatgatt gcctgcggtt
    31921 tcgactacaa aatgtctgtc gaaaaagcct actccacaga ctccaacatg cttggtgcaa
    31981 cgcatgaagc gaaggatctg gaatacctca actccagcgt caaaatcgtc aacccaatta
    32041 tgggcgtgaa attctgggat gagagcgtga agatcccggc agaagaagtc acagtacgct
    32101 ttgagcaagg tcatccggtg gcgctgaacg gtaaaacctt tagcgacgac gtagaaatga
    32161 tgctggaagc taaccgcatc ggcggtcgtc acggcctggg catgagcgac cagattgaaa
    32221 accgtatcat cgaagcgaaa agccgtggta tttacgaagc tccggggatg gcactgctgc
    32281 acattgcgta tgaacgtctg ttgaccggta ttcacaacga agacaccatt gagcagtatc
    32341 acgcgcatgg tcgtcagttg ggccgtctgc tgtatcaggg gcgttggttt gactcccagg
    32401 cgctgatgct gcgtgactct ctgcaacgct gggttgccag ccagatcact ggtgaagtta
    32461 ccctggagct gcgccgtggg aacgattatt caatcctgaa taccgtctca gagaacctga
    32521 cctacaagcc agagcgtctg acgatggaaa aaggcgactc ggtgttctcg ccagatgatc
    32581 gtattggtca attgaccatg cgcaacctgg atatcaccga tacccgcgag aaacttttcg
    32641 gttatgccaa aactggcctg ctctcctcct ctgccacttc aggcgtgccg caggtggaga
    32701 atctggaaaa taaaggccag taattcgaca tcaaccctgc ccttcagggg tggggttacc
    32761 ttgcttatcc tctgtaaact cctttctgag catcacataa tcgaaaacat catggtttgc
    32821 atccacaacc cgtgagtctc ctttatagtg tgcaatcact tcctccagcg tttgcggctg
    32881 ttccggcttt gttaccccca tccacgcatt aataaggtaa ttattgtacg cagtggaaaa
    32941 gatatcgttt tctgttgtgc gatccacgcc atccccaaga acggggctat accagataaa
    33001 catcgggaca tgatatgcct gctggctggc ctccctgcct ccatgaaaat agacgttctt
    33061 cttcgtaggg tcgcgttcca ggccgtggtc ggcaaaatac atgaccgagg cgcggcgatc
    33121 ttttagtaat tcaaaaacct gacctagcaa actgtctgtg taatgaatgg agttgtcata
    33181 gcaggcatcc tgatcgtcct gcggttgaaa cacggcgctg gattgcggat aggcgctaca
    33241 agccggttca tggcttccat ttaaatgaag aacaatcagt ttcttttgct gcgtcttttg
    33301 ctgtaacgct tgcgataaat gcggcaacaa caattcatca aatcctctga catagactgt
    33361 ttccatggcg cgcatggcga tactggtaac tgctgtacca ttctgccgaa aagcggattg
    33421 cgagcttagc cagaaagtct gaaatcctgc ttgattagcc atattaataa tgttgtcggg
    33481 gtaattatga atgtcatgac tcaaaacaga atcagcagtt aaagaaaggg gaaccgacag
    33541 cgcagtgtaa ggtgcgccgc ttattgcttg attaaacagt ttgatctgtt ttctttgtgc
    33601 ttcaacttgc ggtgtcgtag agcgtgtata tccatacaaa gacatattgt cgacacgtac
    33661 agactccccc acaatcaaca cgtaggtatc aatacctgta tccctgactg ataattgaaa
    33721 atacggcacc gtgtttgcaa ttgagagtaa tctttgatgc tctttcgctg ctaaagcaaa
    33781 atagttgaga ttaaaaaacg gcgtataggt agcaaatcgc gacgctagta tatatggact
    33841 gaacgctttt ttattttttg catctttata agcaaattga caagcggaaa ataaactgcc
    33901 cgaaatgaca atcagcaata atattcctgt cacttttttt gtcggcaagg aaacatcata
    33961 ttttattatt actgccaaaa aaagaaggga taaaaaagca aaagcacata gataagggat
    34021 atacatcccc agcattttga caacttcatc cggatcactc tgcagcacac taatcgcaaa
    34081 tccatcattg aaagttgtac caaaggtaca ccatgaatag aggctaatac tcatgtctgc
    34141 cgcagtcagt aacacaaatg gtgcggcaac aagcaggcga agcggaaggg atgatatacg
    34201 tttaacaacc aggatggtca ggacgaagaa cagcaatcgc tgaaccatat gcgctccgga
    34261 agaggcgact aagttcgtaa taccaaaaac aataacacaa agatacaaca accaatgaga
    34321 tttaaaactt ctagcaaatt tgttgaatac tgtcatccag aaatccaagt tcacaaccaa
    34381 tataaacaat tatcaacaat gttcaatttt atatcagaca tgtttttcaa ctctgatcca
    34441 tatcagaccg aaacactgtt agttttttct ttttaaatga tgcggccatt gttctcagta
    34501 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    34561 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    34621 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    34681 gtaccggcac catcgtttca aagtaccagc tacgagtaaa gcaactggac gagatacaga
    34741 tacctgacaa ccattcctca gaccaggacc aaaacgaaaa aagacgcttt tcagcgtctc
    34801 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    34861 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    34921 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggc
    34981 gcactcaggt tttcccattc gctaccttta ttggttttat tgctattgat gttacccagc
    35041 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    35101 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    35161 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    35221 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    35281 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    35341 taaccaaagc gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    35401 aaacagcaaa accgatgtgt tcgctgagaa aaaaggcgac aatcgccgcc tttttagcca
    35461 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    35521 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    35581 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    35641 ctgactcatg ctcaagtgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    35701 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    35761 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtagtttta tccagtagga
    35821 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    35881 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    35941 caagccccat gttgctcatc aatgtaccca cagcgccacc acgcagctgg ccctgacgaa
    36001 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    36061 cgaccataat cacgcgatcg ccatcgccgt cgaaggcaat accgagatcc gctttttcag
    36121 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    36181 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    36241 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    36301 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    36361 tacggctggc tttacccagt tctgccgaat caacgcagct gatctccttt tccatttccg
    36421 cttcgatggc ctcttctacc gcatccggca gtttggtgcc gtcgatagag aagaatttaa
    36481 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    36541 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    36601 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    36661 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    36721 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    36781 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    36841 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    36901 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    36961 accacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    37021 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    37081 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    37141 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    37201 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    37261 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    37321 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    37381 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    37441 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    37501 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    37561 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    37621 tccagtgaag taccctgggc aaagagtttc atgatgttat ccctggtatg aattgataag
    37681 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    37741 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    37801 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    37861 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    37921 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    37981 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    38041 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    38101 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    38161 gggtcaccat gttacgtgcc agattggtcg caactttaat atcgttggac gcaccggtag
    38221 aaacatgttc cggcccatag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    38281 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    38341 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt gtgcaccgga tcgtgttccg
    38401 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    38461 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    38521 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    38581 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgggca atgattgccg
    38641 cgtcgatatc gggggccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    38701 cgcgaacatc tggcaagccg accacaacct gacggtcgaa acggccagga cgcagcaggg
    38761 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    38821 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    38881 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    38941 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    39001 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    39061 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    39121 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    39181 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    39241 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    39301 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    39361 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    39421 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    39481 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    39541 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    39601 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    39661 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    39721 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    39781 gtttacgccc ggtcgctaca atatacactt cccgcgaacg tgcacgagaa gagtccggct
    39841 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    39901 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    39961 ctaacgccag ttccaccaga tacatggcac gggggatatc caccgccggt gttccgctca
    40021 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    40081 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    40141 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    40201 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    40261 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaacccct
    40321 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    40381 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    40441 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    40501 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    40561 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    40621 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    40681 atcgccaccg aagatcgcga aactaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    40741 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    40801 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    40861 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    40921 gcatagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    40981 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    41041 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    41101 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    41161 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    41221 ttggtgacat caatcacctg cgcgttcgac agcttggctt cgatgtcttt aatacggcct
    41281 tcgcagaaac cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    41341 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    41401 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    41461 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    41521 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    41581 ggtcagctac aattcacatt ttgatagtca ttttaccctg aagttcccga agggtcatcg
    41641 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    41701 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    41761 gccgcgaatg ttgatgagta cgttactcaa ctccccgctg gtgccaacct tgccctgatg
    41821 gtgcaaaaag tcggcgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    41881 cccgccagta cgcagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    41941 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    42001 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    42061 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    42121 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    42181 tttagcgctc cgcctgccgc tgccatagtt gaccgcaact gtttctccgt ctcgctctac
    42241 agtgccccaa aacctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    42301 atgttcagcc aggtacgcac cctcccccga ggttctgccg aagcgcaata ctgcgaactg
    42361 gatgtcgtac caggcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    42421 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg ggcaattctg
    42481 aaagatgagt taaaacaggc aggtatcacc tggagcggaa cactgctgcg ccagactcag
    42541 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgttgca cgatctgctt
    42601 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgataggc
    42661 catgcgcgct tcaatgtgcc tggaacatgg cgggccgggt cggacgccgt gcgtcagatc
    42721 ctgcgccagc aagccggtgt cgatattgga aacaccatta ttgccgatgg ttcagggctt
    42781 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    42841 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    42901 cagtaccgtg caggtctgca tcaggcgggc gtggatggaa aagtctcagc gaaaaccggt
    42961 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    43021 gcgtttgtac aatatctttc tggctatgca gtagaaccgg cggatcagcg taatcgccgt
    43081 attccgttag tgcgttttga aagccgtttg tataaagata tttatcagaa caattagtca
    43141 aaaagaaacc ccggcacatg gccggggttt tgctgattaa cgcttgtaaa tgaactcaac
    43201 gccttcttcg tcgtcttcgt cccagtcatc atcccagtcc tcatcatcct cttcagcaat
    43261 ctcttcaagc tgctggcgat gataatcatc ccacatgaat tcgactttct ctggctgttt
    43321 cgcttcttca gcctgcacga ccgggttttc aatgataaag gtcatcacat cccagcagag
    43381 atctttcacg cccagtccac tcgccgcaga gatcagataa tatttatctt cccagcccag
    43441 cgcttcagca atcgctttcg ctttctcttc agcttctgcc ttatccagca gatcgatctt
    43501 attgaacact aaccaacgtg gtttcgccgc cagatcctgg ctgtattttt ccagctcgct
    43561 gataataata cgcgcgtttt caaccggatc ggtgccgtca atcggatcga tatcgatgag
    43621 gtgcaacagg acgcggcaac gttccaggtg cttcaggaag cgaatgccca gacctgcgcc
    43681 ttccgcagcg ccttcaatca gtcctggaat atcggcaaca acgaagctct tttcgttgtc
    43741 cattcgtacc acacccagac ttggcaccag agtggtaaac ggataatccg ccactttcgg
    43801 tttagccgcc gataccgcac gaataaaggt cgatttaccc gcgtttggca tccccaacat
    43861 accgacgtca gccagcagca tcagctccag cagcagctca cgcttatcgc ccggcgtacc
    43921 gttagttttc tgccgtggtg tacggttaac tgacgatttg aaacgggtat tgcccagacc
    43981 gtgccagccg cccttagcaa ccagcagacg ctgaccgtgt ttggtcatat cgcccatggt
    44041 ttcaccagta ccctggtcga ttacacgcgt acctaccggc actttaattg tgacgtcttt
    44101 accgcgctta ccggtacagt cgcggcttgc gccattctga ccgcgctctg cacggaaaga
    44161 tttttcaaaa cgataatcga taagcgtgtt caggttctcg tcggcttcca tccatacgtc
    44221 accaccatca ccgccgtcgc cgccatccgg gccgcctttc ggaatatact tttcgcggcg
    44281 gaagctcacg caaccattac cgccatcacc tgcaacgacc agaatcgatg cttcatcaac
    44341 aaacttcatt ttattctccg taaatcattc gcctgcgcgg ggttgcgata ccaccgttgt
    44401 atgcttacgt aatccgcccc aaatacgatg accaatggcg gaatacatcg cgcccgcaac
    44461 cacgacaaac gcaccgagat aacctaaaag gtttaacatc ggtctggcga agaaatcggg
    44521 tcaggccagt gataaaagat ctgaaaaaaa cagcgtaaac agtggggtga gcgtgatgat
    44581 cgcgctcacc tgcgctgcct gccagcgagc catcgcttcc gccagggcgc catatcctac
    44641 caaggtattc agtccgcaaa aaattaaaca tgcgagctgc cagtggctaa gctgcgctat
    44701 cactccaggc tttgccagag ggaagagcgc aattgtacat aaagtgtaca gtaaaaacag
    44761 gatctgcggt gaggccagcc gacgcaataa aaccttttgc gccacgccat aactcaccca
    44821 aaccgtcgcc gcaccgaccc caaagataac tccccaggtg taatcggtga gctttgtaaa
    44881 tatctcgacc agactggtgt taaaaaacat caccaggccg ctcaggagca tcaatgcccc
    44941 tacaacctga gtgctgcgca ttttctcttt caggataaat acgctggcaa ccatcatgcc
    45001 aactggcgag agttgcccaa tcacctgcga agcggtcgga ctcaggtatt gcaaggatga
    45061 gctgaacagg atgaagttcc caaacagccc ggcggtcgcc actgccaaca aaatcaacca
    45121 gcgtggctta cgaaacacgc gtaatggcgg caacctcttc ttcaccgcaa gaatggcacc
    45181 aaggccaata ctcgccatca agaaacggta aaacacgatt gtcggaggtt ccatcacctc
    45241 cagcacctgc ttcattgcga ttggcaacgc cccccagcaa attgctgtgg tgagcgccaa
    45301 aagaatgcca atgcctgcct gctgcttcat accgtattcc ctgcaaagcg catttgcgga
    45361 ttaacgtcgc accatgcgca tttttaccgg ttatcgaatg taaaaagccc cgcaacgtgt
    45421 tgcggggctt tcatccgtta ccgggacgcg aaaaacttat tcagcttcga tgctgataaa
    45481 tttacggttt ttcgggcctt taacttcgaa tttcacttta ccgtctgctt tagcaaacag
    45541 agtgtggtca cgaccgcaac ctacgttagc gccagcgtgg aatttggtac cacgttgacg
    45601 aacgatgatg ctacccgcca gaacggattc gccaccgaaa cgcttaacgc ccaggcgttt
    45661 agcttctgaa tcgcgaccgt tacgtgtgga gccgccagcc tttttatgtg ccatttgaaa
    45721 tctctcctca ggtcttaggc gctgatgcca gtaattttca catcagtgaa ccactgacga
    45781 tggccctgct gcttacgata gtgtttacga cgacgaaact taacgatttt aactttctcg
    45841 ccacgaccgt gagcaacaac ttcagctttg attacgccgc catcaacgaa aggaacgccg
    45901 attttgactt cttcaccgtt tgcgatcatc agcacttcag cgaactcaac agtttcgcca
    45961 gttgcgatgt ccagcttttc caggcgaacg gtctgacctt cgcttactcg gtgttgttta
    46021 ccaccacttt ggaaaaccgc gtacatataa aactccgctt ccgcgcacac cttttcaatg
    46081 attcagagtg cgctataaat attcacaata gggcgcgaat attacgcaaa acgcacgcct
    46141 ttgacaagtg ctacagtcaa tacacgaaga aaaaaaacac aacttgtacg gtaacgttta
    46201 tctgtgccat tttttcagta caatcactct atattcctaa ccataaaccc taagttgcct
    46261 ttgttcacag taaggtaatc ggggcgaaaa gcccggcttt tgcgatgaat ttagaaaaaa
    46321 tcaatgagtt aaccgcgcaa gatatggcgg gtgttaatgc ggcaatcctt gagcagctta
    46381 attccgacgt ccagctgatc aatcagttag gctattacat cgtcagcggc ggcggtaaac
    46441 gtattcgtcc gatgattgct gtactggctg cacgagctgt tggctatgag ggaaatgcgc
    46501 atgtcaccat tgctgccctg atcgagttta tccacacggc gactctgctg cacgacgacg
    46561 ttgtggatga atcagatatg cgcaggggta aagctaccgc caacgctgca tttggcaatg
    46621 ccgccagcgt gctggtaggc gattttattt atacccgcgc gttccagatg atgaccagcc
    46681 tcggttcgct caaagtgctg gaagtcatgt cagaagccgt aaacgtcatc gcagaaggtg
    46741 aagttctgca actgatgaac gttaacgatc cggacatcac tgaagaaaac tacatgcgcg
    46801 ttatctatag caaaaccgcg cgtctgtttg aggctgccgc gcagtgttcc gggattctgg
    46861 ctggctgtac gccggaagag gagaaaggcc tgcaggatta tgggcgctat ctcggcactg
    46921 ctttccagtt gatcgacgat ttactcgatt acaatgccga tggcgaacag ttaggtaaaa
    46981 atgtcggcga cgatctgaac gaaggtaaac cgacgctgcc gctgctgcat gcgatgcatc
    47041 atggcacacc agaacaggca cagatgatcc gtaccgccat cgaacagggt aacggtcgcc
    47101 atcttctgga accggttcag gaagcaatga acgcttgtgg atctcttgaa tggacgcgtc
    47161 agcgtgccga ggaagaagca gacaaagcca tcgcagcatt acaggtgctc ccggacaccc
    47221 cttggcgaga agcactcatc ggcctcgcgc acatcgctgt tcaacgcgat cgttaatccc
    47281 ctcccttcat cccgcgcagt gcgcgggatg attccagtaa attccataaa aacactaatt
    47341 cagcgctata acacctgaaa aaaagggcga tgaatcttta tatattctga atattcacac
    47401 tctttacagg aactttttag agcaataggc catcaggagt atagtgatgc ccgacagaag
    47461 aagtgttctg aatgaaagcg actaacttaa ggagtgagga aagatggaaa gtaatttcat
    47521 tgactggcat cccgctgaca tcattgcggg tttgcgcaag aagggaacgt caatggcggc
    47581 ggagtctcgc agaaatggtt tgagttcctc aacgctggca aacgcattat cgcgcccatg
    47641 gccgaaagga gagatgatta ttgcgaaagc cctgggaact gacccctggg ttatctggcc
    47701 atcacgctac catgatccgc agacccatga gtttatcgac agaacgcagt tgatgcgtag
    47761 ctacactaaa ccgaaaaaat gagtggtctg gcggtagccc cgcgaacggg gctgccagct
    47821 ctcagacgat tactcgcctt tcacacgctc aatatttgca cctaaagcgc gcagtttgtc
    47881 ttcaatgcgt tcgtagccac gatcgatgtg ataaatacga tcaaccaccg tcgtcccttc
    47941 cgcaatacag ccagccagca ccaggcttgc tgatgcacgc agatcggttg ccataacctg
    48001 tgcgccagaa agtttttcaa caccgtgaca aataacggta ttgctttcga tttcggcatg
    48061 cgcgcccata cggctaagct ccggcacatg cataaagcgg ttttcaaaga ccgtttcggt
    48121 gatgaatccg gtcccttctg ccaccaggtt caacagcgtg aactgggcct gcatatcggt
    48181 cgggaatgcc ggatgcggcg cggtacgtac gttaacagcc ttcggacgtt tgccatgcat
    48241 atccaggcta atccagtctt cgccgacttc gatgtccgct ccagcgtcac gcagtttcgc
    48301 cagcacggcg tcgagagtat ctggctgcgc gttacggcag ataattttgc cgcgagaaat
    48361 cgccgccgcc accaggaaag taccagtttc gatacgatcc ggtagcacgc gatagacacc
    48421 gccgcctaaa cgttccacac cttcgatgac gatacgatcg gtgccctgac cgctaatttt
    48481 cgcacccagc gtaatcagga agttcgcggt atcgacgatt tccggttcac gcgctgcgtt
    48541 ttcaataatc gtggtacctt ccgccagggt tgcagcacac atgatggtca ccgttgcgcc
    48601 aacgctgact ttatccatca cgatatgtgc gcctttcaga cgaccatcga cggaagcttt
    48661 aacgtaacct tcttccagtt tgatggtcgc gcctaattgt tcgaggccag aaatgtgtag
    48721 atcaaccgga cgcgcaccga tcgtacaacc gccaggcagt gaaacttgcc cctgaccaaa
    48781 gcgcgctacc agcggcccca gtgcccagat agaagcacgc atggttttaa ccagatcgta
    48841 aggtgcgcag aatacattaa cgtcgcgggc atcaatatgc acagaaccat tacgttctac
    48901 tttcgcaccc agctggctta gcagcttcat tgatgtatcg acgtctttca gtttcgggac
    48961 gttctggatc tctaccggtt cttccgccag tagagcggca aaaaggatag gcagagcagc
    49021 atttttagcg ccggaaattg tgacttcgcc ctggagcttc gttggcccct gaacacgaaa
    49081 tttatccatt tagtttgttc tcagttaaca attcatatcc gctaccggcg aatcgcccat
    49141 agctcaaaag ccgttcagtt tgcgatcgcg cgcccactcc gcaggggtat acgctttgat
    49201 cgacacagca tgaatgcggt tatccgcaat atattccatc agcgggccat agaccgtctg
    49261 ctgtttttta acccgactca tgccgtcaaa caactcaccc acggcaataa cctgaaagtg
    49321 gctgccatcg ccggaaacgt ggacttcctg gagggagaga gcgttcatca acacgctctg
    49381 aatttcatta ttttccatgg gatcttcaat catcagttaa taaaccagcg aaacatctta
    49441 gagcaaagtt gcgctggcat aaataagcaa aaagcctcgc tgataaatca gacaaggctc
    49501 gacttgcagg caggtttgcc ggacaggcgg ttaacgccat atccggcctg aaaaaattta
    49561 acgaggcaga acatcagcag gcaaattata caatttcgcg agggtataca ctttgtcgtt
    49621 taccccctga agcgtcacgt tgttgccctg ctttttcgcc agatcgataa gatggagcag
    49681 cagtgccagt ccacccgtat ccacgcggga cacacggcta agatcgatgc aggtaatccc
    49741 cttcaccgct tcctcacgca tttcccaaag cggtagcaga acgtcctgat ccagctctcc
    49801 ggataacgcc agcgtgtcac ccgtctgcat ccagctcagt gactcgctca ttattttttc
    49861 tcttccagag tgattttctg ttgagaaatc gatttcagtt gcgcagtcag gccgtcgata
    49921 cctttggtac gcagcagcgt tccccactcg ttttgtttgg tggtgatcat actgacgcct
    49981 tcagcaatca tgtcgtaagc ctgccagttg cccgtctggg agtttttacg ccactggaag
    50041 tccagacgca ccggcggacg gccattcggg tcaataatgg taacgcgaat aggcacaatg
    50101 gttttatcgc ccagcggctg ttctggcgca atctgatagg tttgaccgtg atacatcgcc
    50161 agcgcctgac cgtaagcctg cttcaggtac tcacggaaag cggcaaagta ggcttcacgt
    50221 tgagcagggg tcgcactctt gtaatactgg cccagcacca gcgcaccggc gtatttcacc
    50281 tgtacgtatg gcagcagttc ctgatcaaca atggtacgca gataatccgg gttggcccga
    50341 atttgcggtt gctcattctt caggcgatcg aacgttttct gcgccgcctc gtccatcagc
    50401 ttataaggat tggtctggtc tgccgcggtt gccgcactca gaggtgcaat caccagcaaa
    50461 gcgaccatca ttaaacgttt aaacatgcgt cggttctcct gaaattattt cgttgtaccc
    50521 acaggttcag tggtttcatt attacctggc gcagcagctg gcgcatcgcc actattctta
    50581 ttgtcatcgc ctttactacc gtaaaggaac tgaccaatga gatcttccag caccatcgca
    50641 gacttagtgt cctgaattgt atcgccatcc ttcaggatag cagtccccag ttccgggtct
    50701 tcaaaaccga cgtttaatgc cagatattgt tcccccagca ggccggaagt acgaatgctc
    50761 agcgaactgg tatctggaat gtggttataa cgttgttcaa tttccagcgt tacgcgcggc
    50821 agataggttt tcgggtccag cgtaatatcc gccacccgac ccacaacaac gccaccaata
    50881 ctgaccggag agcgcgcttt caggccgcca atgttatcga acgtcgcata aagcgtgtag
    50941 gtcggttcag tacgtatgga cgtcacgttc gccgccttca ggcaaacaaa cagcgccgcc
    51001 agcagtgctg ctaataaaaa gatacccacc caaatttcat tttttttcgt ttgcatgaac
    51061 tcaattccca aacatcaatg cggtcagcac aaaatccagc cccagaacag ccagagacga
    51121 gtggacaacg gtgcgagtgg ttgcccggct aatcccggca gacgtcggga tggcgtcgta
    51181 gccgttaaac aacgaaatcc acgtcaccgt gatggcgaac accacgctct taatcagaca
    51241 gttgaccaga tccatacgcc agtcgacggc attttgcatt gccgaccaga agaacccgct
    51301 atcaatgcct ttccaactga cgccgaccag agatccgccc cagatcccca cggcgacgaa
    51361 aataaccgtc aacagtggta atgaaataac cccagcccag aaacggggag aaataacccg
    51421 acgcagcgga tccaccgcca tcatctccat actggagagt tgctctgtag cgcgcatcag
    51481 gccgatttct gcggttagcg ccgaaccagc acgcccggca aacaacaacg cggcaaccac
    51541 cggccccagt tcacgcagta gcgataacgc caccagcata cccagactgg tttccgcact
    51601 ataagtggtc agaaccagat aaccttgcag ccccaacacc attccgatga acacgccaga
    51661 aaccacaata atcagcatcg acaggacgcc gacattatag agctggcgca ccagcagcgg
    51721 cgcatgtttg cgaaattccg gtttgccgac cagcgcattg aataacatta acccggcccg
    51781 cccgaacgtt ctcagggttt taatcccttt atgtccgagc gacgccagcg catttaacag
    51841 catgagtggc ttaactccct ggtaaaagat cagcgtgata atcgccggca ggatagcgga
    51901 acggaacagg cccgtcagct atcccgtcca gaaactgacg tacgcgcgga tcaggattcg
    51961 cctgcaacgc ctgggcactg ccatgagcga caattttttt gtccgccagg atccaggcgt
    52021 gatccgcaat acttaacact tccggcacat cgtgagaaac caccacacaa gtcacgccca
    52081 gcgcgctgtt cagctcagaa atcagcttca ccagtacgcc catggtgatg ggatcttgcc
    52141 caacaaaagg ttcatcaaac atgatgagat ccggctccag cgcaatcgca cgcgccagcg
    52201 ctgcacgccg cgccatccca ccagaaagtt cagaaggcat tagcttagcc gctccacgca
    52261 gccccacggc ctccagcttc atcatcactg tactatgcaa caatggcgcg ggaagttggg
    52321 tatgttcgcg cagtggatag gcgacgttgt caaatacgtt catatcagtg aacaacgccc
    52381 cggactgaaa taacatgctc atccgtttgc gcactgtata caggcgcgaa cgagacatcg
    52441 ccggaatatt ctcaccatcg aaaaggatct caccatgatc tggtgcgatt tgcccgccaa
    52501 tcagacggag tagcgtcgtt ttaccgatgc ccgatggccc catgattgcc gtgatcttcc
    52561 ctcgcggcac ggtcagggaa atattatcga agatgcagcg attgccacgc gtaaaactga
    52621 catcgcgcat atcgactaaa ttcgccacag actgctccat aattcaccct tcgtcttgcg
    52681 ttgattttct aagcatggcg ctcaatttaa ccttgaaccc aacatattta cagaatatta
    52741 cccgccgtgg ttagcgaaag ctggcatttg ttttactttt tagccgcata aagtcaaaat
    52801 taagaatccg ttacggcttt ctgaaaatct tcagcggacc ggcgagtata cctgaagaaa
    52861 ggacgttaga tgcttttagc tacggcactg ttaattgttg gtttactttt ggtcgtttac
    52921 agtgccgacc gcctggtttt tgccgcgtct attctttgcc gaacctttgg catcccgccg
    52981 ctgatcatcg gcatgacggt ggtcagtatt ggtacatcgt taccagaaat catcgtctcg
    53041 cttgccgcgt ctctgcacga acaacgcgat ttagccgttg gtacagccct cggctcaaac
    53101 attatcaata tattgctgat cctcggtctg gctgcgctgg ttcgtccttt taccgtccat
    53161 tctgatgttc tacgccgtga attaccctta atgttgttgg tcagcgtggt ggccggttcc
    53221 gtactctatg acggacaact tagtcgcagc gatggtatct ttctcctctt tctggctgtg
    53281 ctatggctgc tgttcattgt taaacttgca cgtcaggctg aacgtcaggg gactgacagc
    53341 ctgaccagag agcagcttgc agagctgccg cgtgacggcg gattgcccgt cgcgttttta
    53401 tggctcggca ttgcgcttat catcatgcca gtggccacgc ggatggtggt tgataacgcc
    53461 acggtgctgg cgaattactt tgccatcagc gagttgacga tggggctgac ggcaattgct
    53521 atcggaacca gcctgccgga actggcaacc gcaatagcgg gggttcgcaa aggtgaaaac
    53581 gacattgctg tcggaaatat cattggcgca aacattttta atattgtcat cgtgttgggt
    53641 ttacccgcgc tgataacgcc aggagagatt gatccactgg cgtacagtcg tgactacagc
    53701 gtgatgttgc tggtgagcat tatttttgcg ttgctgtgct ggcggcgctc cccgcaaccg
    53761 ggccgtggtg taggggtatt attaactggc ggatttatcg tatggctggc gatgttgtac
    53821 tggttatcgc caatactcgt tgaataactg gaaacgcatt atgtcgcacg tagagttaca
    53881 accgggtttt gactttcagc aagcaggtaa agaagtcctg gcgattgaac gtgaatgcct
    53941 ggcggagctt gagcaataca tcaatcagaa tttcacgctt gcttgtgaaa agatgttctg
    54001 gtgtaaaggg aaagttgtcg tcatggggat ggggaaatcg gggcacatcg ggcgcaaaat
    54061 ggcggcaacg tttgccagca ccggtacacc ttcatttttc gtccatcctg gtgaagccgc
    54121 gcatggtgat ttaggcatgg ttaccccaca ggatgtggtg attgctatct ctaactcagg
    54181 tgaatccagc gaaatcacgg ccttaattcc agtgcttaag cgtcttcacg taccgttaat
    54241 ctgcatcacc ggtcgcccgg agagcagcat ggcgcgcgcc gcagatgtgc atctgtgtgt
    54301 taaagtagcg aaagaagcct gtccgttagg gctggcaccg accagcagca ccaccgccac
    54361 gctggttatg ggcgatgccc tcgctgtcgc gctgttaaaa gcacgcggct ttactgctga
    54421 agattttgcg ctctcacacc caggcggcgc actgggtcgt aaacttctgc tgcgcgtaaa
    54481 cgatattatg catacgggcg atgagatccc gcatgttaag aaaacggcca gtctgcgtga
    54541 cgcattgctg gaagttaccc gcaaaaatct tggtatgact gtcatttgcg atgacaatat
    54601 gatgattgaa ggcatcttta ccgacggtga tttacgccgt gtcttcgata tgggcgtgga
    54661 tgttcgtcag ttaagtattg ccgatgtgat gacgccgggg ggaatacgtg tgcgccctgg
    54721 cattctggcc gttgaggcac tgaacttaat gcagtcccgc catatcacct ccgtgatggt
    54781 tgccgatggc gaccatttac tcggtgtgtt acatatgcat gatttactgc gtgcaggcgt
    54841 agtgtaaaga ttcaaggata aacaacaatg agcaaagcag gtgcgtcgct tgcgacctgt
    54901 tacggccctg tcagcgccga cgttatggca aaagcagaga acattcgtct gctgatcctc
    54961 gacgttgatg gcgtactgtc agatggcctg atttatatgg gcaataacgg cgaagagctg
    55021 aaagcgttca atgttcgtga cggttatggc attcgttgtg cgctcacctc tgatattgaa
    55081 gtcgctatca ttaccgggcg aaaggctaaa ctggtagaag atcgttgtgc cacattgggg
    55141 atcactcact tgtatcaggg gcagtcaaac aaactgatcg cctttagcga tttgctggaa
    55201 aaactggcga ttgccccgga aaatgtggct tatgtcggcg atgatctcat cgactggccg
    55261 gtaatggaaa aagtgggttt aagcgtcgcc gtggccgatg cgcatccact gttgatcccg
    55321 cgcgccgatt acgtgacgcg cattgctggc ggtcgtggcg cagtgcgcga agtttgcgac
    55381 ttattactcc tggcgcaggg caaactggat gaagccaaag ggcaatcgat atgagtaaag
    55441 ccagacgttg ggttatcatt gtgctatcac tggcggttct ggtgatgatc ggcattaata
    55501 tggccgaaaa agacgatacc gcccaggtgg tcgtcaacaa caatgatccc acctataaaa
    55561 gcgagcatac ggacacgctc gtctataacc cagaaggggc actaagctat cgattgattg
    55621 ctcaacacgt tgaatattat tccgatcagg ccgtttcgtg gtttacgcag ccggtactta
    55681 ccacgtttga taaggataaa atcccgacat ggtccgtaaa agcagataaa gccaagctga
    55741 ccaatgaccg gatgctctat ttatatggac acgttgaagt caacgcactc gtgccggact
    55801 ctcaacttcg cagaatcacg acggataacg cgcagatcaa tctggtgacg caggatgtta
    55861 cctctgaaga cctcgtcacg ttatacggaa caacatttaa ctccagcggt ctgaaaatgc
    55921 gcggcaactt acgcagcaag aacgccgagc tgattgaaaa ggttagaaca tcctatgaaa
    55981 ttcaaaacaa acaaactcag ccttaatctt gtgcttgcca gctcacttct ggccgccagc
    56041 attccggcat ttgccgtaac cggagacact gatcagccga tccacattga atcggaccag
    56101 caatctcttg atatgcaagg caacgtggtt acctttaccg gtaatgtcat cgtcacccag
    56161 ggcaccatca aaattaatgc cgacaaagtg gttgttaccc gtccgggcgg cgaacaaggt
    56221 aaagaagtga ttgacggcta cggtaaaccg gcaacgttct accagatgca ggacaacggt
    56281 aaaccggttg aaggtcacgc ttcccagatg cactacgaac tggcaaaaga ttttgtcgtt
    56341 ctgacgggta atgcttatct gcagcaggtt gatagcaaca ttaagggcga taagatcact
    56401 tacctggtaa aagagcagaa aatgcaggct ttcagcgaca aaggcaagcg cgtaaccacc
    56461 gttctggtgc cgtcgcagct gcaggacaaa aacaacaaag gccagacccc ggcacagaag
    56521 aagggtaatt aattcgttat ggcaacatta actgcaaaga accttgcaaa agcctataaa
    56581 ggccgtcgcg tggtagaaga cgtcagcctg accgtcaact ccggggaaat tgtcggtctg
    56641 ctggggccaa acggtgccgg taagaccacc actttctaca tggttgtagg cattgtgccg
    56701 cgcgatgcgg gcaacatcat tattgatgat gacgatatca gtctgctgcc tctgcatgca
    56761 cgcgcgcgcc gcggtatcgg ctatctgcca caggaagcct ccattttccg tcgcctcagc
    56821 gtttacgata acctgatggc ggtactgcaa attcgtgacg acttgtctgc tgaacaacgt
    56881 gaagaccgcg cgaacgagct gatggaagag tttcacattg agcacctgcg tgacagcatg
    56941 gggcagtcac tctccggggg tgaacgtcgc cgtgtagaaa ttgcccgcgc actggctgcg
    57001 aatccgaaat ttattctgct cgacgaaccg tttgccgggg ttgacccgat ctcggttatc
    57061 gacattaaac gcatcattga gcacctgcgc gacagcggcc tgggcgtgct gatcactgac
    57121 cacaacgtgc gtgaaacact ggcggtttgt gaacgcgctt atatcgtcag tcaggggcat
    57181 ttgatcgccc acggcacgcc tacagaaatc ttacaagacg aacacgttaa gcgtgtatac
    57241 cttggggaag acttcagact ctgatagggt agaagtttgc gacgttttag caggagagta
    57301 cgattctgaa catgaagcaa ggtttgcaac tcaggcttag ccaacaactg gcgatgacgc
    57361 cacagctcca acaggcaatt cgtctgttgc agttgtcgac gctggaactt cagcaggagc
    57421 tacagcaggc gctggagagt aatccgctgc ttgagcaaat cgacactcat gaagaaatcg
    57481 acacccgcga aacgcaagac agtgaaacgc tggacaccgc cgacgcgctc gaacaaaaag
    57541 agatgccgga agagctgccg ctcgatgcca gttgggacac catttacacc gctggtacac
    57601 catccggcac cagcggtgac tacattgacg atgagctgcc ggtctatcag ggcgaaacga
    57661 cgcagacctt gcaggattac ctgatgtggc aggtcgagct gacaccgttt tccgacactg
    57721 accgcgctat tgctacctct atcgtcgatg ccgttgatga caccggttat ctgactgtcc
    57781 cgctggaaga tattctcgaa agtatgggcg atgaagagat cgacatcgac gaggttgaag
    57841 ccgtccttaa gcggatccaa cggtttgatc cggtcggtgt agcggcaaaa gatctgcgtg
    57901 actgcctgct aatccaactc tcccaattcg ataaaaccac gccgtggctg gaagaggcca
    57961 gactgatcat tagcgatcat ctcgatctgt tagccaatca cgacttccgc actttaatgc
    58021 gcgtcacgcg tctgaaagaa gatgtgctga aagaagccgt caatctgatc cagtcgctcg
    58081 atccgcgccc cgggcagtcg atccagactg gcgaacctga gtatgtcatt ccagatgtgc
    58141 tggtgcgtaa gcataacggt cactggacgg tagaactcaa cagtgacagc attccgcgtc
    58201 tgcaaatcaa ccagcactac gcctcgatgt gcaataacgc gcgcaacgat gatgacagcc
    58261 agtttatccg cagcaatctg caggatgcca aatggttgat caagagtctg gaaagccgta
    58321 acgatacgct actgcgcgtg agtcgctgta tcgttgaaca gcagcaagcc ttctttgagc
    58381 aaggtgaaga atatatgaaa ccgatggtac tggccgatat cgcccaggct gtcgaaatgc
    58441 atgaatcgac gatatctcgc gtgaccacgc aaaaatacct gcatagtcca cgaggcattt
    58501 ttgaactgaa gtatttcttt tccagtcacg tcaataccga gggcggcggc gaagcttcct
    58561 ccacggcgat tcgtgcactg gtgaagaaat taatcgcggc ggaaaaccca gcgaaaccgt
    58621 tgagcgacag caagttaacc tctttactgt cggaacaagg tatcatggtg gcacgccgca
    58681 ctgttgcgaa gtaccgagag tctttatcca ttccgccgtc aaaccagcgt aaacagctcg
    58741 tttgacccaa ccgataagga agacactatg cagctcaaca ttaccggaaa taacgtcgag
    58801 atcaccgagg cactgcgcga atttgttaca gctaaatttg ccaaacttga gcaatatttt
    58861 gaccgaatca accaggtcta tgttgttctg aaagtggaga aagtcaccca cacctcagat
    58921 gcaacactgc atgtaaacgg tggtgaaatt catgccagcg cggaaggtca ggacatgtac
    58981 gctgccattg atggcttaat tgataagctg gcacgccagt tgaccaaaca taaagataaa
    59041 ctgaaacaac actaattgtc cgggcaatta gcatgtgcat ggcggcctgt tgtgcggcac
    59101 aacgggccat ttgtacggtt aatgctccga gcctgttcca ctgtttgagt gggcaggttc
    59161 ttaggtgaaa ttatgacaaa taatgataca actctacagc ttagcagtgt tcttaacagg
    59221 gaatgtacgc gaagccgcgt ccactgtcag agcaaaaaac gcgccctgga aatcatcagc
    59281 gagctggcgg cgaaacaact tagcctgccg cctcaggtgg tttttgaagc tatcctgacg
    59341 cgggaaaaaa tgggcagtac cggtatcggc aatggtattg ccattccgca tggcaaactg
    59401 gaagaagata ctctgcgcgc cgttggcgtt ttcgttcagc tcgaagcgcc tatagctttc
    59461 gatgccatcg acaaccaacc ggtggactta ctttttgccc tgctggtgcc ggcagaccaa
    59521 actaaaacgc acctgcatac tctgtcgctg gtggcgaaac gtctggcgga caaaaccatc
    59581 tgccgccgtt tacgcgcagc ccagagcgat gaagagctgt atcaaatcat tacggatacc
    59641 gaaggtactc cggatgaagc gtagttattc ggtaatgtct cttttagacg ttgtgaggag
    59701 aaacagtaca tggtactgat gatcgtcagc ggacgttcag gttcaggtaa atctgtcgcc
    59761 ctgcgtgcgc tggaagatat gggtttttac tgcgtggata accttcccgt agtgttgtta
    59821 cccgatctgg ctcgaactct ggccgatcgc gagatttctg ccgccgtcag cattgatgtt
    59881 cgtaatatgc cggagtcacc agaaatattc gaacaggcga tgagtaacct gcctgacgct
    59941 ttctcaccgc aactactgtt cctggatgcc gaccgtaata ccttaattcg tcgttacagt
    60001 gacacgcgcc gactgcatcc gctttccagc aaaaacctgt cgctggaaag tgctatcgac
    60061 aaagaaagcg atttgctgga gcctctgcgt tcgcgagcgg atctgattgt cgatacctca
    60121 gaaatgtccg ttcacgagct ggctgaaatg ctgcgtaccc gtctgctggg taaacgtgaa
    60181 cgcgaactga ccatggtctt tgagtctttc ggcttcaaac acggtatccc tatcgatgca
    60241 gattacgtct ttgacgtgcg cttcttgccg aacccgcact gggatccgaa actgcgtcca
    60301 atgacaggtc ttgataaacc tgtcgccgcg ttcctcgacc gccacacaga agtacacaat
    60361 tttatctacc agacgcgaag ctatcttgaa ctatggttac ctatgctgga aaccaacaac
    60421 cgtagctact tgacggtcgc cattggttgt accggcggga agcaccgttc ggtgtatatt
    60481 gcagagcaac tggcagacta cttccgctcg cgcggtaaaa acgtccagtc acgccatcgt
    60541 acgctggaaa aacgtaaacc atgaccgtca agcaaactgt tgaaatcaca aacaagctgg
    60601 gcatgcatgc ccggcctgca atgaagctgt ttgaattaat gcagggtttt gacgctgaag
    60661 tgctgttacg taatgacgaa ggcaccgagg ccgaagccaa cagcgttatt gcgctgctga
    60721 tgttggattc tgccaaagga cggcagattg aagttgaagc gaccggccca caggaagagg
    60781 aagcactggc cgccgttatc gccctcttta actctggttt tgatgaagat taatcttcat
    60841 cactttgaca tacaactccc ttcaaactcc ccccgacaat aagaaaatca cgtactgaaa
    60901 tcgttctcaa tcaacgtcat ttgtacattt tgtgcgcttt tcactttcag aagaacctta
    60961 agaaaacctt aagaggcatt gtttaggttt tgtttaagtt aatcgaccat actggagatc
    61021 gtcagaaaat atttccagga gatggcatga ttcgcttatc tgaacaaagt cccctgggca
    61081 ccggaagaca tcggaaatgt tatgcgcatc cggaagatgc ccaacgctgt atcaagattg
    61141 tctaccatcg tggcgatggt ggcgataaag agatccgccg tgagttaaag tactacgcgc
    61201 atcttggtcg ccggttaaaa gactggagtg gaataccgcg ctatcacggt accgttgaga
    61261 cggattgcgg aaccggttat gtctacgatg tgattgcaga ttttgacggt aaaccctcca
    61321 ttacgctgac cgaatttgct gaacaatgcc gttacgaaga agacattgca caactgcgcc
    61381 agctactgaa acagctgaag cgttatttgc aggataaccg tatcgtgacg atgtcgctga
    61441 agccgcagaa tatcctctgt caccgcatca gtgagtcaga ggttatcccg gtcgtctgtg
    61501 acaacattgg tgaaagcaca ttgattccac tggcgacctg gtcgaagtgg tgttgtctgc
    61561 gtaagcaaga gcggttatgg aaacgattta tcgcccaacc ggcgctggct attgccctgc
    61621 agaaagatct gcaacctcgt gagagcaaaa cgctggccct cacttcgcgc gaagcttaat
    61681 ccagctggtg ttgttgcata aacggctcac cgcctaactg atacatctgc cgtaaaatcc
    61741 acgcctgacg gctacgcacg tagcccgatg gcgaggagac tttgaaacga agtggattag
    61801 gtaatacagc tgccagtaat gcagcttccg accgggtaag tttgctcgcg ggtttgtgga
    61861 aataacgttg tgccgcagct tcgacgccaa acacaccgtc gccaaattcg gcgatattca
    61921 ggtaaaccgt caggatacgc tttttgctcc agactgtttc tatccccagc gttaatccag
    61981 cttccagccc ttttcgtacc cagctacgcc catcccataa aaagagattt ttggccgtct
    62041 gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg tgcgccaggg
    62101 ctttctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga tcttctgcgg
    62161 caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag tcagaatgtg
    62221 ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc attaccgctg
    62281 agaagggaac aggcgcaaca ctaaacaacg cgatgccccc gccccagaaa acggcgagga
    62341 caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag cggcttttac
    62401 tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg ccgcttctgc
    62461 aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt catcaaccac
    62521 gatatcatcg acaggacagg gcacatgctc cgcgcccatc tcttccagca cttctgcggt
    62581 atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg gcagcatcgc
    62641 cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt gtgccagcgc
    62701 ctttaattca cggtcaacgg tgcattcgct accaagagtg gcaaaattgc ttaaattctt
    62761 cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat cggcctgggc
    62821 cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacgt tgcgcgtttc
    62881 cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat ccggtgcaaa
    62941 gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg cttcatgaat
    63001 ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct tcattgtgat
    63061 catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt acgcatcaca
    63121 catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg cgtgcggcct
    63181 gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag ccaagatttc
    63241 cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt ttagtggctt
    63301 ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc atctcttcaa
    63361 tccattcacc gacgttatct tcccaggcag gaaggtccgg agactgaatt tgctgaccca
    63421 gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta tgtccttcct
    63481 ctacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg acatagcccg
    63541 gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct acaagttcga
    63601 gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc tcttctgtcg
    63661 tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc gcggttagcg
    63721 ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg tactcttgtt
    63781 tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg cgcggataac
    63841 gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga atatccagca
    63901 acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg gtcatggcga
    63961 catcaacgct gttacctaat ttttccagca cagaacgcgc aacaatcacg ttcagttcaa
    64021 tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca tcaaacgcat
    64081 catcgacctc ttctgctacc gacggtgcgt ggatcgtcaa cgtaaaggtt gaacctttgc
    64141 cctgttcgct ggtaaccgta atatcgccgc ccatattttt cgccagacga cgagaaacgg
    64201 ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc acctggtaat
    64261 acatggcgaa aattttatcc agctcatcct gcggaatgcc gataccagag tcttccactt
    64321 caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg ccttgctggg
    64381 tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc gtcccgtcgg
    64441 taatgacctg atgcggtaat ggcagcgtcg gctccaggtt aaagcacaat cctttttgtt
    64501 gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa tcaaccggtt
    64561 gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata tcgttaaaga
    64621 tattccccag cgtgacggcc gaaacatgga tggtcttgag atatttttcc tgctcggcgg
    64681 tgagttcggt atccagcaga atacggctca ggccgacgat accgttcagc ggtgtacgca
    64741 attcgtgact gatggtggaa ataaacgtcg ttttgtcgcg gctggcccgt tcaagcgcat
    64801 cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg tgacgtttac
    64861 ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc ccgtccgggt
    64921 aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt tcatcggttt
    64981 caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg tgaaccagtt
    65041 gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa aactctttat
    65101 cttcgttacg ataaaaaacc aggtcgggtg aagcatcaag gaaggaacgt aagaatgagg
    65161 attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt ttcagttggc
    65221 cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc tgatttagct
    65281 gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc atctcctcca
    65341 gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg acgaccaccg
    65401 atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa cgaataacat
    65461 caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc acaatggcaa
    65521 gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac ttcatcatca
    65581 ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc cttcacgaca
    65641 acctgcccca ataataccca attctgacag ttacgttata aattgtgtga gaaatgcgga
    65701 attcccctca acgacctact ccgttggagg tatccacgga cgttccagcg ccgatccctg
    65761 tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc gattttcgca
    65821 ccacagagga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt ggtagatcac
    65881 ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtgtaatcct ccagttcaat
    65941 accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca caatatgcag
    66001 cggatgcagc tttatgccgt ccacgccggt ttcaaccact cgctccagcg tttgcaaaca
    66061 ttcagcctgc ccttcgccag gcaggccgac aattaagtgg gaacacacct tcagcccgcg
    66121 ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc ggttgatgcg
    66181 atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt cgtaaccctg
    66241 gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg ggcgggtacc
    66301 aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag aacgcagcac
    66361 ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc gtttagcgcg
    66421 gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct gctgcgcttc
    66481 atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg taccgtcacg
    66541 gttagggcag ctaaaaccgc cgtgtagcgt cagcttatgc accttttgcc cataacgacg
    66601 ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct gatagaccgc
    66661 gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga cctggatcaa
    66721 tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa tttcatgcat
    66781 aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct tatggatttt
    66841 aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa tgctggtttt
    66901 ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag gaattgactg
    66961 tttctctaac gacttccctt ttagccttaa agataaaatc cattttaatt tcagtaattt
    67021 aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat ttgacctgta
    67081 tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca gcctgctcat
    67141 catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg aaaaggacaa
    67201 caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac gctctttctg
    67261 tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag gttcacgata
    67321 tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc gcccacatag
    67381 aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc cgcatgcagc
    67441 accgtggcgc gattctcgcc gatggtaaaa ccggcgacgg ttgcggcttg ctgttacaaa
    67501 aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta gcaaaaaact
    67561 acgccgtcgg gatgctattc ctgaataaag atcctgaact cgccgctgcc gcacgccgca
    67621 tcgttgaaga agaactgcaa cgcgaaacct tgtcgattgt gggctggcgt gatgtcccca
    67681 ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt gagcaaattt
    67741 ttgtgaacgc cccggcaggc tggcgtccgc gcgatatgga gcgccgtctg tttatcgccc
    67801 gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt agcctgtcga
    67861 atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc ttttatctgg
    67921 atcttgcgga cctgcgtctg gaatcggcca tctgcctgtt ccaccagcgc ttctccacta
    67981 acaccgtacc gcgctggccg ctggcgcagc cgttccgcta cctggcgcat aacggtgaaa
    68041 tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa ttccagacgc
    68101 cgcttatccc tgatctgcac gacgccgcac cgttcgtcaa tgaaaccggc tctgactcca
    68161 gttcgatgga taacatgctg gaactgctgc tggcaggcgg aatggatatc atccgcgcca
    68221 tgcgtctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg gaactgcgtg
    68281 ccttctttga ctttaactcc atgcatatgg agccgtggga tggcccggcg ggcatcgtga
    68341 tgtccgacgg tcgttttgcc gcctgtaacc tcgaccgtaa cggtctgcgt ccggcgcgct
    68401 acgtcatcac caaagataag ctcatcacct gcgcctctga agtcggtatc tgggattacc
    68461 agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg gttatcgaca
    68521 cccgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa agccgccatc
    68581 catataaaga gtggatggag aaaaacgtcc gccgactggt accgtttgaa gatctgcccg
    68641 atgaagaagt gggtagccgc gaactggacg acgacacgct cgccagctat cagaaacagt
    68701 ttaactacag cgcggaagag ctggactccg taattcgcgt actgggcgaa aacggtcagg
    68761 aagcggtcgg ttcgatgggc gacgacaccc cattcgccgt gctctccagt cagccgcgca
    68821 ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca atcgacccgc
    68881 tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg aacgtctttt
    68941 gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg ctctactccg
    69001 atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg ctggatatca
    69061 cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctgtgc gacaaagccg
    69121 aaaaaatggt acgtagcggc accgtgctgc tggtgctctc cgaccggaat atcgctaaag
    69181 atcgcctgcc ggttccagcc ccgatggcgg ttggcgcgat ccagacccgt ctggtcgatc
    69241 aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc gatccgcacc
    69301 acttcgccgt gttgctgggc ttcggcgcga cggctattta tccatacctt gcctatgaaa
    69361 cgctgggccg cctggtagac acccatgcga ttgccaaaga ttatcgtacc gtgatgctca
    69421 actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg ggcatctcca
    69481 ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctgcac gatgatgtag
    69541 tgggcctgtg cttccagggg gcggtcagcc gcattggtgg ggcgagcttt gaagacttcc
    69601 agcaggatct gctgaacctg tcgaaacgtg cctggctggc gcgtaagccc atcagccagg
    69661 gcggcctgct gaaatacgtc cacggcggcg aataccacgc ttacaacccg gacgtggtgc
    69721 gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa tacgcgaagc
    69781 tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg ccgggtgaaa
    69841 acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc tttgataccg
    69901 ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg gaagcgatga
    69961 atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg cgctatggca
    70021 ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt actccggcgt
    70081 atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag ccgggcgaag
    70141 gcggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc tattcggtgc
    70201 ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc gaggacttag
    70261 cgcagctcat tttcgacctc aagcaggtta acccgaaagc gatgatctcc gtgaagctgg
    70321 tttccgaacc gggcgtaggc accatcgcga ctggcgtggc aaaagcttat gccgatttaa
    70381 tcaccatcgc aggctatgac ggtggcaccg gcgcaagccc gctgtcgtca gtgaaatacg
    70441 caggttgccc gtgggagctg gggcttgttg aaacccagca ggcgctggtt gccaatggtc
    70501 tgcgtcataa gatccgtttg caggtcgatg gcggcctgaa aaccggtgtt gatatcatca
    70561 aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccgatg gtggcgctcg
    70621 gctgtaaata tctacgtatt tgccatctga acaactgcgc aacgggtgta gcaactcagg
    70681 atgacaaact gcgtaagaac cactatcacg gcctgccgtt caaggttacc aattactttg
    70741 aatttattgc ccgtgaaacc cgcgagctga tggcacagct gggcgtaacg cgtctggtgg
    70801 atctgattgg tcgcaccgac ctgctgaaag aactggacgg tttcaccgcc aaacagcaaa
    70861 aactggcgct gtcgaagctg ctggagaccg ccgaaccgca tccaggtaag gcactctact
    70921 gcaccgaaaa caacccaccg tttgataacg gcctgctgaa cgcgcagttg ctgcaacagg
    70981 cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatt cgcaacaccg
    71041 accgttctgt cggcgcgtcg ctttcaggct atatcgccca gacgcacggc gatcaggggc
    71101 tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt ttcggtgtgt
    71161 ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat gtcggtaaag
    71221 gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc cgcagccatg
    71281 aagcaagtat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt ctgtatgccg
    71341 caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc gtggtagaag
    71401 gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt ctgggtaaaa
    71461 ccggcgttaa cttcggtgcg ggcatgaccg gcggcttcgc ttacgttctc gatgaaagcg
    71521 gtgatttccg caaacgcgtt aacccggaac tggtcgaggt cttaagcgtt gacgatctgg
    71581 cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat actggctctc
    71641 agcgcggtga agagattctg gcgaactggt caaccttcgc cactaaattt gcgctggtta
    71701 aaccgaagtc cagtgatgta aaagcactgc tgggtcatcg tagtcgtagc gcagcagagt
    71761 tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat caatttatcg
    71821 acctgcagcg cgttgatccg ccaaagaaac cgctgaagat ccgcaaaatt gagtttgttg
    71881 aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc tgcctgtcgt
    71941 gcggcaaccc atactgcgag tggaaatgtc cggtacacaa ctacatcccg aactggctga
    72001 agctcgccaa cgaggggcgt atttttgaag cggcggaact gtcgcaccag accaacaccc
    72061 tgccggaagt ttgcggacga gtctgcccgc aagaccgtct gtgcgaaggt tcctgcactc
    72121 tgaacgatga gtttggcgcg gtgaccatcg gcaacattga gcgttatatc aatgataaag
    72181 cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt aaaaaagtgg
    72241 cgattatcgg cgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg cgtaacggcg
    72301 taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc ttcggtattc
    72361 cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc actggcatgg
    72421 gtattgaatt caaactcaat accgaagtgg gccgcgacgt gcagctggac gatctgctga
    72481 gtgattacga tgccgtcttc cttggcgtcg ggacttatca gtcaatgcgc ggcgggctgg
    72541 aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc aacaccaaac
    72601 agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa ggcaaacgcg
    72661 tggtggtcct tggcggtggc gacactgcga tggactgcgt gcgtacgtcc gtgcgccagg
    72721 gagcgaagca cgttacctgt gcctatcgtc gtgatgaaga taacatgccg ggttcccgcc
    72781 gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc cagccactgg
    72841 ggattgaagt gaacggtaac ggtaaagtca gcggcgtaaa aatggtacgt accgaaatgg
    72901 gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc gaacatatcg
    72961 ttccggcaga tgcagtgatc atggcgtttg gtttccgtcc acacaacatg gaatggctgg
    73021 caaaacacag cgtcgagctg gattcacaag gccgcatcat cgccccggaa ggcagcgaca
    73081 acgccttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc cgtggttccg
    73141 atctggtggt aaccgctatt gccgaaggtc gtaaggcggc agacggtatt atgaactggc
    73201 tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg ctgaaattct
    73261 cctttctttc tggaaagcgc ctcgcataat ctgaagaaaa ccaatgtaat acgctcatct
    73321 ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg cagcgccata
    73381 taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac cagcaaatcc
    73441 acgacggtat tattcatctg gtcgatagcg cccggacgga aacggtacgt agcgttaacg
    73501 cgctgatgac cgcgacatac tgggaaattg gccgacgaat tgtcgaattt gaacaaggtg
    73561 gcgaggccag ggctgcgtat ggtgcgcagc taatcaagcg actatcaaag gatttaagtc
    73621 taaggtataa gcgtgggttc tctgcaaaaa acttacgcca aatgaggctt ttttacctct
    73681 tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca ttgggaattc
    73741 cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg tctgccaaat
    73801 ctttccccct gccctggtcc acctacgtcc gtttactttc cgttaaaaac gctgacgccc
    73861 gcagctttta tgaaaaagag acgctccgct gtggctggtc tgttcgccag ctagaacggc
    73921 aaattgcgac ccaattttat gagcgaacac tactgtcaca tgacaaatca gccatgctgc
    73981 aacaacacgc tcctaccgag acgcatattc ttccgcagca ggcgatacgc gatccctttg
    74041 tgctcgaatt tctggaattg aaagatgaat actcagaatc cgattttgag gaggcgctga
    74101 tcaaccacct gatggatttc atgctggaac ttggggatga ttttgccttt gttggtcgac
    74161 agcgaaggtt acgcattgat gacaactggt ttcgtgtcga tctgctgttt ttccaccgcc
    74221 gtttacgctg cctactaatc gtcgatctaa aagtgggcaa attcagctat agcgatgccg
    74281 gacagatgaa tatgtatctc aactacgcca aagagcactg gacgctaccg gatgaaaatc
    74341 cgcccatcgg tctggttctc tgtgcagaga aaggagccgg agaagcacat tatgctctgg
    74401 caggtttgcc taacaccgtt ctggcaagcg aatataagat gcaactacct gatgagaaac
    74461 gactcgcaga tgaactcgtt cgaacacagg cggtgctaga ggaaggctat agacgccgtt
    74521 aatttcagcg gtttcgaatt ctgcagacag tgtctgcaga atctttaaaa cgaactaagt
    74581 tttaagccat taaatcagcc ttaaccttca ccacaacctt tttaatctct ccaggctcgc
    74641 cgacaacgca tcctggttta tgcggttcac ctggcataaa cacggcgaac attcccggtt
    74701 ttaagatgat ggtttgctcg ttttcaatgg tgctgcaaag ctgataatca tcctcatggt
    74761 ggaactcttc acactggcgc gcagtgcctg ccatgccaaa cagaatccgt tcctcaccgt
    74821 ttaataacag ttggatatca atgtattgct cgtgcaattc cgcttttttc tcgacgggcg
    74881 attgagtgtt aaacgtcatg acattcataa agatattgtc gccctgtaat tcgtaacgac
    74941 ccggcgcttt ttcttgcggc ctggcggcta atgccagcgt taacgcgtcc tgtaacgcag
    75001 gatgtaaccc agcagacggt aatgactgta cttcacccat catcataatt tttctccctg
    75061 ggccaacagc gcagccccaa gtaaacctgc atcatggcgg tagtgcgccg ccagtaaatc
    75121 aacatgaaat gccgctggct cctgcgccag atacgtttcc accagcgcca gatacccttc
    75181 tgccagacca acgctgccac cgaccaccac gcactggcaa tcagttgtgg ctttaatatc
    75241 agcgatcagc cttgcaagcg tacgtgcgga gcgatggatc agccattgcg cttgttcgtc
    75301 gccctgcccg gcgcgtgtaa aaatagttct cgcatccgcg cccgccagtt caccctgcgc
    75361 tgccgccgca atgccgcgac cagaagcaat cgcttccacg caacctgtgc gtccacagcc
    75421 gcagactgga ccatgcggat cggcaagcgt atgcccgata tgccccgcca gaccgccagg
    75481 gccggtaagc agtttaccgc cgctcactac accgccgccg acgccagtgg aaacggtgat
    75541 aaacaccatc tcggttacat cgccttccag cgcctgatac tctgcccatg ctgcggcctg
    75601 cgcgtcgtta atggcaatgg tcggcaaatt ggtaagttgt tccagcgttt tgactaacgg
    75661 aaagtgtagc aatccaccga gattatgcgg attcagcgcc agcaaactgc cgtcgcgaat
    75721 aattccggtt gaagcgatgg caacccgctg cgcatgagct tgcaacggag agactaatgc
    75781 ggataaggca tcacgcaagg cttctggtgt ctggctggct ggcgtaggaa gttcacgacg
    75841 atcgcggatc tgcccgtcag cgccaatcag cgcggcggca agtttagtac cgccgatatc
    75901 aatcgccagt gtggtcatag caccgccttt ttcatcgctg tgttgtacca ctgacaaatg
    75961 tgttcaagac gcgtaatggc agaaccgacc gtcaccgccc acgcgccgtg gcgcatcgca
    76021 tctgccgcct gagcaggcgt gttgtaacgc ccttcggcaa tcacccgaca tccggcgtcg
    76081 ctcaacgttt tcaccagcgc cagatccggc tcttctggcg tttcaggcgt ggtatagcca
    76141 gaaagcgtag tgccaataat ttcggctccc agcttttggc atgccaggcc gtcttccggc
    76201 gttgagcagt cggtcatcgc cagtaaaccg tgatggtgaa tacgtgccag cagcgtttca
    76261 acaggcaccg gacgcgggcg gtcggtgccg tcaatggcga taatgtccgc gcccgcctgc
    76321 gccagcgcat cgacatcttc aatataggcc gtgatgcgta ccggagaatc ctccagatcg
    76381 cgtttcacaa ttccaataat cggcacgctc accaccgcac gcgtggcttg cagatttgcc
    76441 acaccttcaa tacgaatggc gaccgcgccc gcctgctctg ccgctaatgc catggcggcg
    76501 acgatttcgg gtttatcgag cgggctgtca ggaaccggct ggcaggagac aatcaggcca
    76561 ccgttagcag cgattttttg atccagttgt gcaagtaacg acatacatct tcccttagcg
    76621 aaaggcccgg tacagagacc gggcaacagg attaactttt ggttttgact aaatcgtttt
    76681 tggcgctgcc aaacggcacg gcaccgctga atggtttacc gtcgatagcg tcatgagtac
    76741 gcaacgcttc cgggcgcaac caacgctgaa cgcgagaagg catatccagc ccaatcagca
    76801 ggatcaccac gaacgtcaga ctgaacgaga gcgatgccag cgcagtaccc agatccagac
    76861 gttgagcgat caacgcgccg atgattgggg ccagtgcacc gcccaatgcg ccaacgttgt
    76921 aggtaaagcc caggcccgct gcacgctggt cggtatcgaa ataaccgcca atcagttttg
    76981 gtaagatccc ggcgatccct tgtccaagca tttgctggaa gaacagtaac agaccgagca
    77041 cccagacgtt tgcgccgcca atcgcaaata ccggaataat cagcagctgc gaggccagca
    77101 ggctacaaac gtacgctttg cgggttccca gccagtcacc gaggaagcca cctacgcagc
    77161 atcccaccgc cgcgccaaag ccactaaaga acagcacatt ggctacagta tgcgggttat
    77221 aagccagatc ggttttcaga tacgttggca gcagcgcctg aatcggccat gagtagagga
    77281 aagcaaacaa cacgaccacc atcagcatta cgcccgttgg ccagcgtttg cctgtactct
    77341 gcaccataaa gctgataaag attgcggcgc ataacagccc aagaacagcg acgatcgcgg
    77401 cattttgcag gttaccggcg aagcagaacc acagcgcagt agccgccgcc agtgtcatta
    77461 cgatattggc aatgcgatgt tcaccacggt agagaatatc caccattgtg cgtactggtg
    77521 ctttacctgc gtgtttctct ttccagtctt ccgcttccgg gatgttttta cgcagccaga
    77581 gagcaaagat gattggcaaa atgccgataa agaacagcgc acgccagccc cagaccggaa
    77641 ccaccaggct atagacctga gcggcaacga cggcccccac agagaagcct gaaatcagaa
    77701 aaccactggc tttgttacgc agatgttttg gccagctttc aatgacatag gtggcgctgg
    77761 aaccgtattc acccgccatc cccatgccga tgaccagacg cgcgataaac atggtgatgt
    77821 agcctggcgc aaagccgcag gccagcgtcc cggccgagaa gagaacgatg ctggtgacca
    77881 ttgccagacg acgcccgtag cggtcaccca tagcgccgag catcaggccg ccgaaccagc
    77941 gagagataaa ggctgcggag atcagacttg ccgcctgcac cgtcgtcagc ccgaattcac
    78001 cctgtacttc ggtgagtacc agggcgatta aaacgaaatc aaaaccgtca agcagatatc
    78061 ccaaccaggc agcggaaaat gcgcgccatt gtgcacggtt gagatggcga taccacggga
    78121 tattctgggt tgtagtactc attgtgagtc tcccgcggtg ggcgatgccc acacgctttg
    78181 gtataaaaat tgtagggtac agatgcgttt atttcccctc acccggtagg ggcgagcgag
    78241 gggaaacaac tcacccgcgc tcttgcatca actgctgggc cagcgccttc agttctggca
    78301 gatatttttc atctaccggt ccaaacggtt tgcggcacag cggcacagaa acgacatcca
    78361 tataatggag gacagttttc aggccgcgga atacgcccgt tttgatcagt aaatcaatga
    78421 ctttattgca ttcagtttgc agtttctgcg cggtctggat atcgccttct ttcagcgcct
    78481 taacgatccc ctgatagcgc cagcccatga tgttgtaggt actaccgata ccaccatcag
    78541 cgcccgccag cagaccagag gcgaagattt cgtcgtaacc gttatagagc acaagatcag
    78601 gatgttcacg acggatctgc tccatctgat agagatcgcc agaggtctgt ttcagcgcac
    78661 ctacgccagg caatgtaaca agtgtgttga tctgatccag ggtcagtttt accccactca
    78721 gggctggaat gttgtacacc accatcggca aaccatccgc cgaatcaata attgcccgat
    78781 agtgatcgca gtgttcttca aagctgaaag gatagtagaa cggcgtgacg gcggagacgg
    78841 catcgaagcc ataacgttta gccgatgccg caagttgttg gctttcggcg gtgctgacgc
    78901 aaccgacgtg ggcgatgagt ttaatcttac ctttcgcctc ttcggcgacg atttccagta
    78961 cctgttcacg ctcggaaagg ctttgtacaa aggcctcgcc ggtcgaacca cccacgtata
    79021 aaccgtcgat gccctgctga atattgaact gaaccaggcg acgcagactc gctttatcca
    79081 gtgcttgttg ttggtcaaaa ggagtcagga gtgcagccat tacgccacgt aaattcgttg
    79141 ccataaatac ctctgaagtg atgcttgtct gataaacgat atacctttat acctgttata
    79201 ccagatcaat taagcaacac cccatacaga aagcttataa tgcgatctgc ttcactaaag
    79261 tggcattatt tctttttgtt ggtggtctga ccgaaagcgt gccaggtagc agagacgctg
    79321 ttgagatgcg attgcaacgc acgatcggct tcgtcaggat catgacggcg gatcgcatca
    79381 acgatcgcaa tatgctgttg ataactaacg ttgttatgtt cgtgcagtgc ctgatcggta
    79441 accgttgggc gtgcggcaat aagccagtcg agcagggcaa cgtggatcgc catgaagatt
    79501 gggttaccgg ggatctccgc cagcacgcgg tggaaatcaa cgtctgaacg aatgaatgcc
    79561 gcgttgttat ccagcgactg actgttgatt tccagtgctt ttgccagcaa atcgatttgc
    79621 tcatcggtgg catgttcagc cgcatagcgc accaggctgg attcaaagaa cagacgtaat
    79681 tgttcgaaat gggcaatccc accaggatga gaaaggaaat ctttcgccat gccggaaagc
    79741 tcgccgatga tggtgtccgc agaaggacgt gagacgcggg cacgttcgcc gttgttgatt
    79801 tgcaccagac ctttgcgttt taacgctgcc agcgcttcgc gcacagaagg acgcccgacg
    79861 ttaaagaacg ccatcagttc gcgttcagac ggtaattgtt caccttcgcc aaattcacga
    79921 cggcggatca tctgttccag ctcttcttcc accatttcgg agagtttttt acgcgccagc
    79981 gggcggctac gcaagttgcg accaattgca ggtgaagaat cttcggtttg cgaatcaaat
    80041 gcgttcataa ggcccattct gtaaggtcag tgtgattaac atcatcagtg acatcctatc
    80101 acaggattga aagtagggga aaatggcagg tttttctctt tgtgcctcat cattaccata
    80161 attaacggaa taattaacta ttgcgaaaa
//
LOCUS       DAZFXT010000008        73392 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.008, whole genome shotgun sequence.
ACCESSION   DAZFXT010000008 DAZFXT010000000
VERSION     DAZFXT010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73392
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.008"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(217..480)
                     /gene="cedA"
                     /locus_tag="ACHKW0_000578"
     CDS             complement(217..480)
                     /gene="cedA"
                     /locus_tag="ACHKW0_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HFO7473463.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            663..2924
                     /gene="katE"
                     /locus_tag="ACHKW0_000579"
     CDS             663..2924
                     /gene="katE"
                     /locus_tag="ACHKW0_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HFO7473464.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDSTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2971..3729)
                     /gene="chbG"
                     /locus_tag="ACHKW0_000580"
     CDS             complement(2971..3729)
                     /gene="chbG"
                     /locus_tag="ACHKW0_000580"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HFO7473465.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLNV"
     gene            complement(3742..5094)
                     /gene="celF"
                     /locus_tag="ACHKW0_000581"
                     /gene_synonym="chbF"
     CDS             complement(3742..5094)
                     /gene="celF"
                     /locus_tag="ACHKW0_000581"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO7473466.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5199..6041)
                     /gene="chbR"
                     /locus_tag="ACHKW0_000582"
     CDS             complement(5199..6041)
                     /gene="chbR"
                     /locus_tag="ACHKW0_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HFO7473467.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6049..6399)
                     /gene="chbA"
                     /locus_tag="ACHKW0_000583"
     CDS             complement(6049..6399)
                     /gene="chbA"
                     /locus_tag="ACHKW0_000583"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HFO7473468.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6450..7808)
                     /gene="chbC"
                     /locus_tag="ACHKW0_000584"
     CDS             complement(6450..7808)
                     /gene="chbC"
                     /locus_tag="ACHKW0_000584"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HFO7473469.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
     gene            complement(7893..8213)
                     /gene="chbB"
                     /locus_tag="ACHKW0_000585"
     CDS             complement(7893..8213)
                     /gene="chbB"
                     /locus_tag="ACHKW0_000585"
                     /EC_number="2.7.1.196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HFO7473470.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8512..8850)
                     /gene="osmE"
                     /locus_tag="ACHKW0_000586"
     CDS             complement(8512..8850)
                     /gene="osmE"
                     /locus_tag="ACHKW0_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HFO7473471.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9052..9879
                     /gene="nadE"
                     /locus_tag="ACHKW0_000587"
     CDS             9052..9879
                     /gene="nadE"
                     /locus_tag="ACHKW0_000587"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HFO7473472.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10109..10996
                     /gene="cho"
                     /locus_tag="ACHKW0_000588"
     CDS             10109..10996
                     /gene="cho"
                     /locus_tag="ACHKW0_000588"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HFO7473473.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(10956..11531)
                     /gene="ves"
                     /locus_tag="ACHKW0_000589"
     CDS             complement(10956..11531)
                     /gene="ves"
                     /locus_tag="ACHKW0_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HFO7473474.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFHWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(11734..12219)
                     /gene="spy"
                     /locus_tag="ACHKW0_000590"
     CDS             complement(11734..12219)
                     /gene="spy"
                     /locus_tag="ACHKW0_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone Spy"
                     /protein_id="HFO7473475.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(12549..13517)
                     /gene="astE"
                     /locus_tag="ACHKW0_000591"
     CDS             complement(12549..13517)
                     /gene="astE"
                     /locus_tag="ACHKW0_000591"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009017 - succinylglutamate desuccinylase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HFO7473476.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(13510..14853)
                     /gene="astB"
                     /locus_tag="ACHKW0_000592"
     CDS             complement(13510..14853)
                     /gene="astB"
                     /locus_tag="ACHKW0_000592"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009015 - N-succinylarginine dihydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HFO7473477.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(14850..16328)
                     /gene="astD"
                     /locus_tag="ACHKW0_000593"
     CDS             complement(14850..16328)
                     /gene="astD"
                     /locus_tag="ACHKW0_000593"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043824 - succinylglutamate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HFO7473478.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVGQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(16325..17359)
                     /gene="astA"
                     /locus_tag="ACHKW0_000594"
     CDS             complement(16325..17359)
                     /gene="astA"
                     /locus_tag="ACHKW0_000594"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008791 - arginine N-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HFO7473479.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDLWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(17356..18576)
                     /gene="astC"
                     /locus_tag="ACHKW0_000595"
     CDS             complement(17356..18576)
                     /gene="astC"
                     /locus_tag="ACHKW0_000595"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HFO7473480.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            19022..19828
                     /gene="xthA"
                     /locus_tag="ACHKW0_000596"
     CDS             19022..19828
                     /gene="xthA"
                     /locus_tag="ACHKW0_000596"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HFO7473481.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            19995..20705
                     /gene="ydjX"
                     /locus_tag="ACHKW0_000597"
     CDS             19995..20705
                     /gene="ydjX"
                     /locus_tag="ACHKW0_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7473482.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSSSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLASEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            20710..21387
                     /gene="ydjY"
                     /locus_tag="ACHKW0_000598"
     CDS             20710..21387
                     /gene="ydjY"
                     /locus_tag="ACHKW0_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YdjY"
                     /protein_id="HFO7473483.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDVTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTLVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            21402..22109
                     /gene="ydjZ"
                     /locus_tag="ACHKW0_000599"
     CDS             21402..22109
                     /gene="ydjZ"
                     /locus_tag="ACHKW0_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HFO7473484.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            22109..22657
                     /gene="ynjA"
                     /locus_tag="ACHKW0_000600"
     CDS             22109..22657
                     /gene="ynjA"
                     /locus_tag="ACHKW0_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HFO7473485.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSNDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            22667..23833
                     /gene="ynjB"
                     /locus_tag="ACHKW0_000601"
     CDS             22667..23833
                     /gene="ynjB"
                     /locus_tag="ACHKW0_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7473486.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKVNPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFSPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            23806..25341
                     /gene="ynjC"
                     /locus_tag="ACHKW0_000602"
     CDS             23806..25341
                     /gene="ynjC"
                     /locus_tag="ACHKW0_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HFO7473487.1"
                     /translation="MVTPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPHALLATLVSTTIAAVGALLIALLVIVALWPGPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPPMDRFGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPSLAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLGWSRAKIFFYVKCPLMLRPVLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGILAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            25341..25994
                     /gene="ynjD"
                     /locus_tag="ACHKW0_000603"
     CDS             25341..25994
                     /gene="ynjD"
                     /locus_tag="ACHKW0_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7473488.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWFNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            26061..27368
                     /gene="ynjE"
                     /locus_tag="ACHKW0_000604"
     CDS             26061..27368
                     /gene="ynjE"
                     /locus_tag="ACHKW0_000604"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HFO7473489.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLYEWIKQHNLKTDAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(27377..27997)
                     /gene="ynjF"
                     /locus_tag="ACHKW0_000605"
     CDS             complement(27377..27997)
                     /gene="ynjF"
                     /locus_tag="ACHKW0_000605"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HFO7473490.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSLQRQ"
     gene            28084..28491
                     /gene="nudG"
                     /locus_tag="ACHKW0_000606"
     CDS             28084..28491
                     /gene="nudG"
                     /locus_tag="ACHKW0_000606"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HFO7473491.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(28457..28729)
                     /gene="ynjH"
                     /locus_tag="ACHKW0_000607"
     CDS             complement(28457..28729)
                     /gene="ynjH"
                     /locus_tag="ACHKW0_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HFO7473492.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            28965..30308
                     /gene="gdhA"
                     /locus_tag="ACHKW0_000608"
     CDS             28965..30308
                     /gene="gdhA"
                     /locus_tag="ACHKW0_000608"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HFO7473493.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(30425..31466)
                     /gene="ynjI"
                     /locus_tag="ACHKW0_000609"
                     /pseudo
     CDS             complement(30425..31466)
                     /gene="ynjI"
                     /locus_tag="ACHKW0_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
     gene            complement(31594..33555)
                     /gene="topB"
                     /locus_tag="ACHKW0_000610"
     CDS             complement(31594..33555)
                     /gene="topB"
                     /locus_tag="ACHKW0_000610"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HFO7473494.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRALL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            complement(33560..34603)
                     /gene="selD"
                     /locus_tag="ACHKW0_000611"
     CDS             complement(33560..34603)
                     /gene="selD"
                     /locus_tag="ACHKW0_000611"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HFO7473495.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(34720..35271)
                     /gene="ydjA"
                     /locus_tag="ACHKW0_000612"
     CDS             complement(34720..35271)
                     /gene="ydjA"
                     /locus_tag="ACHKW0_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HFO7473496.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            35432..37288
                     /gene="sppA"
                     /locus_tag="ACHKW0_000613"
     CDS             35432..37288
                     /gene="sppA"
                     /locus_tag="ACHKW0_000613"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HFO7473497.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            37455..38471
                     /gene="ansA"
                     /locus_tag="ACHKW0_000614"
     CDS             37455..38471
                     /gene="ansA"
                     /locus_tag="ACHKW0_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HFO7473498.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETICKAMSQNLRGEL
                     TPDD"
     gene            38482..39123
                     /gene="pncA"
                     /locus_tag="ACHKW0_000615"
     CDS             38482..39123
                     /gene="pncA"
                     /locus_tag="ACHKW0_000615"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HFO7473499.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(39284..39556)
                     /gene="yeaC"
                     /locus_tag="ACHKW0_000616"
     CDS             complement(39284..39556)
                     /gene="yeaC"
                     /locus_tag="ACHKW0_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HFO7473500.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(39598..40011)
                     /gene="msrB"
                     /locus_tag="ACHKW0_000617"
     CDS             complement(39598..40011)
                     /gene="msrB"
                     /locus_tag="ACHKW0_000617"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HFO7473501.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            40353..41348
                     /gene="gapA"
                     /locus_tag="ACHKW0_000618"
     CDS             40353..41348
                     /gene="gapA"
                     /locus_tag="ACHKW0_000618"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HFO7473502.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            41432..42316
                     /gene="yeaD"
                     /locus_tag="ACHKW0_000619"
     CDS             41432..42316
                     /gene="yeaD"
                     /locus_tag="ACHKW0_000619"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HFO7473503.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(42367..43221)
                     /gene="yeaE"
                     /locus_tag="ACHKW0_000620"
     CDS             complement(42367..43221)
                     /gene="yeaE"
                     /locus_tag="ACHKW0_000620"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HFO7473504.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTDLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISAAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELIMLDKAYPAPK
                     GKTALDMV"
     gene            complement(43311..44057)
                     /gene="mipA"
                     /locus_tag="ACHKW0_000621"
     CDS             complement(43311..44057)
                     /gene="mipA"
                     /locus_tag="ACHKW0_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HFO7473505.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            44493..46427
                     /gene="yeaG"
                     /locus_tag="ACHKW0_000622"
     CDS             44493..46427
                     /gene="yeaG"
                     /locus_tag="ACHKW0_000622"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011411173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HFO7473506.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWSSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            46540..47823
                     /gene="yeaH"
                     /locus_tag="ACHKW0_000623"
     CDS             46540..47823
                     /gene="yeaH"
                     /locus_tag="ACHKW0_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HFO7473507.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            47970..49445
                     /gene="cdgI"
                     /locus_tag="ACHKW0_000624"
     CDS             47970..49445
                     /gene="cdgI"
                     /locus_tag="ACHKW0_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7473508.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     IIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVVIHKPISDSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQELQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            49626..51116
                     /gene="dgcJ"
                     /locus_tag="ACHKW0_000625"
     CDS             49626..51116
                     /gene="dgcJ"
                     /locus_tag="ACHKW0_000625"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0043709 - cell adhesion involved in
                     single-species biofilm formation [Evidence IEA];
                     GO_process: GO:1902201 - negative regulation of
                     bacterial-type flagellum-dependent cell motility [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcJ"
                     /protein_id="HFO7473509.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEKRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            51159..51662
                     /gene="yeaK"
                     /locus_tag="ACHKW0_000626"
     CDS             51159..51662
                     /gene="yeaK"
                     /locus_tag="ACHKW0_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HFO7473510.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(51663..51767)
                     /gene="yoaI"
                     /locus_tag="ACHKW0_000627"
     CDS             complement(51663..51767)
                     /gene="yoaI"
                     /locus_tag="ACHKW0_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YoaI"
                     /protein_id="HFO7473511.1"
                     /translation="MNDQMFVETLIISSSFFAIAVVLVLSVLLIERTG"
     gene            51937..52383
                     /gene="yeaL"
                     /locus_tag="ACHKW0_000628"
     CDS             51937..52383
                     /gene="yeaL"
                     /locus_tag="ACHKW0_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HFO7473512.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(52340..53161)
                     /gene="nimR"
                     /locus_tag="ACHKW0_000629"
     CDS             complement(52340..53161)
                     /gene="nimR"
                     /locus_tag="ACHKW0_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HFO7473513.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVIMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RAMVEMMAKEPVEWGSLGQWACFFAMSERNIARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            53258..54439
                     /gene="nimT"
                     /locus_tag="ACHKW0_000630"
     CDS             53258..54439
                     /gene="nimT"
                     /locus_tag="ACHKW0_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HFO7473514.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            54494..54841
                     /gene="yeaO"
                     /locus_tag="ACHKW0_000631"
     CDS             54494..54841
                     /gene="yeaO"
                     /locus_tag="ACHKW0_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HFO7473515.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(54863..55117)
                     /gene="yoaF"
                     /locus_tag="ACHKW0_000632"
     CDS             complement(54863..55117)
                     /gene="yoaF"
                     /locus_tag="ACHKW0_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HFO7473516.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRYHPAGEK"
     gene            55300..56325
                     /gene="dgcP"
                     /locus_tag="ACHKW0_000633"
     CDS             55300..56325
                     /gene="dgcP"
                     /locus_tag="ACHKW0_000633"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HFO7473517.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLDDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            56358..56456
                     /gene="yoaK"
                     /locus_tag="ACHKW0_000634"
     CDS             56358..56456
                     /gene="yoaK"
                     /locus_tag="ACHKW0_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HFO7473518.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            56459..56533
                     /gene="yoaJ"
                     /locus_tag="ACHKW0_000635"
     CDS             56459..56533
                     /gene="yoaJ"
                     /locus_tag="ACHKW0_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HFO7473519.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(56592..56840)
                     /gene="yeaQ"
                     /locus_tag="ACHKW0_000636"
     CDS             complement(56592..56840)
                     /gene="yeaQ"
                     /locus_tag="ACHKW0_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7473520.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(57068..58276)
                     /locus_tag="ACHKW0_000637"
     CDS             complement(57068..58276)
                     /locus_tag="ACHKW0_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415949.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-guided endonuclease InsQ/TnpB family
                     protein"
                     /protein_id="HFO7473521.1"
                     /translation="MKRLQAFKFQLRPGDQQECEMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSRIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSQFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            58284..58715
                     /gene="tnpA"
                     /locus_tag="ACHKW0_000638"
     CDS             58284..58715
                     /gene="tnpA"
                     /locus_tag="ACHKW0_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HFO7473522.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTRYRRQIFDHDATEK
                     LRAYFSNVCADFEAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGVSGRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCGGAPISVIRQYIEQQQTPG"
     gene            complement(58731..58919)
                     /locus_tag="ACHKW0_000639"
     CDS             complement(58731..58919)
                     /locus_tag="ACHKW0_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HFO7473523.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(58923..59282)
                     /gene="yeaR"
                     /locus_tag="ACHKW0_000640"
     CDS             complement(58923..59282)
                     /gene="yeaR"
                     /locus_tag="ACHKW0_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein YeaR"
                     /protein_id="HFO7473524.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(59453..60091)
                     /gene="leuE"
                     /locus_tag="ACHKW0_000641"
     CDS             complement(59453..60091)
                     /gene="leuE"
                     /locus_tag="ACHKW0_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HFO7473525.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIIRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLTKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(60218..61141)
                     /gene="dmlR"
                     /locus_tag="ACHKW0_000642"
     CDS             complement(60218..61141)
                     /gene="dmlR"
                     /locus_tag="ACHKW0_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HFO7473526.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQYFAEHYQNFSLEHA"
     gene            61244..62329
                     /gene="dmlA"
                     /locus_tag="ACHKW0_000643"
     CDS             61244..62329
                     /gene="dmlA"
                     /locus_tag="ACHKW0_000643"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HFO7473527.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            62580..64190
                     /gene="yeaV"
                     /locus_tag="ACHKW0_000644"
     CDS             62580..64190
                     /gene="yeaV"
                     /locus_tag="ACHKW0_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HFO7473528.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFLTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            64222..65346
                     /gene="yeaW"
                     /locus_tag="ACHKW0_000645"
     CDS             64222..65346
                     /gene="yeaW"
                     /locus_tag="ACHKW0_000645"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase, oxygenase subunit YeaW"
                     /protein_id="HFO7473529.1"
                     /translation="MSNLSPDFVLPENYCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            65402..66367
                     /gene="yeaX"
                     /locus_tag="ACHKW0_000646"
     CDS             65402..66367
                     /gene="yeaX"
                     /locus_tag="ACHKW0_000646"
                     /EC_number="1.14.13.239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase reductase subunit YeaX"
                     /protein_id="HFO7473530.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIVAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(66421..67536)
                     /gene="rnd"
                     /locus_tag="ACHKW0_000647"
     CDS             complement(66421..67536)
                     /gene="rnd"
                     /locus_tag="ACHKW0_000647"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HFO7473531.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(67618..69303)
                     /gene="fadD"
                     /locus_tag="ACHKW0_000648"
     CDS             complement(67618..69303)
                     /gene="fadD"
                     /locus_tag="ACHKW0_000648"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HFO7473532.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(69508..70089)
                     /gene="yeaY"
                     /locus_tag="ACHKW0_000649"
     CDS             complement(69508..70089)
                     /gene="yeaY"
                     /locus_tag="ACHKW0_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HFO7473533.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(70129..70824)
                     /gene="tsaB"
                     /locus_tag="ACHKW0_000650"
     CDS             complement(70129..70824)
                     /gene="tsaB"
                     /locus_tag="ACHKW0_000650"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HFO7473534.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(70882..72792)
                     /gene="yoaA"
                     /locus_tag="ACHKW0_000651"
     CDS             complement(70882..72792)
                     /gene="yoaA"
                     /locus_tag="ACHKW0_000651"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HFO7473535.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLHSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIAIQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            72924..73268
                     /gene="yoaB"
                     /locus_tag="ACHKW0_000652"
     CDS             72924..73268
                     /gene="yoaB"
                     /locus_tag="ACHKW0_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7473536.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT        18080 a        19013 c        17919 g        18380 t
ORIGIN      
        1 cgacgccaca ggatgaacta tcaaaaatag ctcatcatga ttagcaaaac ttaaccattt
       61 taaaataaat aaacaattaa agaaaaaaga tcacttattt atagcaatag ctcgtcaaag
      121 gcagcttttt gttacaggtg gtttgaatga atgtagcaac gaaatgcaga atttcaggtc
      181 atgtaactcc cggcaaaacc gggaggtatg taatccttac tcagtcactt ccccttcctg
      241 gcggatctga tttgcccaac gttgggcaga ttcaggcaca gtaaacgccg gtgagcgcag
      301 aaatgactct cccatcagta caaacgcaac atatttgcca cgcagcatcc agacatcacg
      361 aaacgaatcc atctttatcg catgttctgg cggcgcgggt tccgtgcgtg ggacatagct
      421 aataatctgg cggttttgct ggcggagcgg tttcttcatt actggcttca ctaaacgcat
      481 attaaaaatt agaaaaactg tagtttagcc gatttagccc ctgtacgtcc cgctttgcgt
      541 gtatttcata acaccgtttc cagaatagtc tccgaagcgg gatctggctg gtggtctata
      601 gttagagagt tttttgacca aaacagcggc cctttcagta ataaattaag gagacgagtt
      661 caatgtcgca acataacgaa aagaacccac atcagcacca gtcaccacta cacgattcca
      721 gcgaagcgaa accggggatg gactcactgg cacctgagga cggatctcat cgtccagcgg
      781 ctgaaccaac accgccaggt gcacaaccta ccgcccctgg gagcctgaaa gcccctgata
      841 cacgtaacga aaaacttaat tctctggaag acgtacgcaa aggcagtgaa aattatgcgc
      901 tgaccactaa tcagggcgtg cgcatcgccg atgatcaaaa ctcactgcgt gccggtaacc
      961 gtggtccaac gctgctggaa gattttattc tgcgcgagaa aatcacccac tttgaccatg
     1021 agcgcatccc ggaacgtatt gttcatgcac gcggatcagc agctcacggt tatttccagc
     1081 catataaaag cttaagcgat atcaccaaag cggatttcct ctcagatccg aacaaaatca
     1141 ccccagtatt tgttaggttc tctaccgttc agggtggcgc tggctctgcc gataccgtgc
     1201 gtgatatccg tggttttgcc accaagttct atactgaaga gggtattttt gacctcgttg
     1261 gcaataacac gccaatcttc tttatccagg atgcgcataa attccccgat tttgttcatg
     1321 cggtaaaacc agaaccgcac tgggcaattc cacaagggca aagtgcccac gatactttct
     1381 gggattatgt ttctctgcaa cctgaaactc tgcacaacgt gatgtgggcg atgtcggatc
     1441 gcggcatccc ccgcagttac cgcaccatgg aaggcttcgg tattcacacc ttccgcctga
     1501 ttaatgccga agggaaggca acgtttgtac gtttccactg gaaaccactg gcaggtaaag
     1561 cctcactcgt ttgggatgaa gcacaaaaac tcaccggacg tgacccggac ttccaccgcc
     1621 gcgagttgtg ggaagccatt gaagcaggcg attttccgga atacgaactg ggcttccagt
     1681 tgattcctga agaagatgaa ttcaagttcg acttcgatct tctcgatcca accaaactga
     1741 tcccggaaga actggtgccc gttcagcgtg tcggcaaaat ggtgctcaat cgcaatccgg
     1801 ataacttctt tgccgaaaac gaacaagcag cattccatcc aggtcatatt gttcctggtc
     1861 tggacttcac caacgatccg ctgttgcagg gacgtttgtt ctcctatacc gatacacaaa
     1921 tcagtcgtct tggtgggccg aatttccatg agattccgat taaccgtccg acctgccctt
     1981 accacaactt ccagcgtgat ggcatgcatc gtatggggat cgacactaac ccggcgaatt
     2041 acgaaccgaa ctcgatcaac gataactggc cgcgcgaaac accgccggga ccgaaacgcg
     2101 gcggttttga atcatatcag gaacgcgtgg aaggaaataa agttcgtgag cgcagtccat
     2161 cgtttggcga atattattcc catccgcgtc tgttctggct aagtcagacg ccattcgagc
     2221 agcgccatat tgtcgatggt ttcagttttg agttaagcaa agtcgttcgt ccgtatattc
     2281 gtgagcgcgt tgttgaccag ctggcgcata ttgatctcac tctggcccag gcggtggcga
     2341 aaaatctcgg tatcgaactg actgacgacc agctgaatat caccccacct ccggacgtca
     2401 acggtctgaa aaaggatcca tccttaagtt tgtacgccat tcctgacggt gatgtgaaag
     2461 gtcgcgtggt agcgatttta cttaatgatg aagtgagatc ggcagacctt ctggccattc
     2521 tcaaggccct gaaggccaaa ggcgttcatg ccaaactgct ctactcccga atgggtgaag
     2581 tgactgcgga tgacagtacg gtgttgccta tagccgctac ctttgccggt gcaccttcgc
     2641 tgacggtcga tgcggtcatt gtcccttgcg gcaatatcgc ggatatcgct gacaacggcg
     2701 atgccaacta ctacctgatg gaagcctaca aacaccttaa accgattgcg ctggcgggag
     2761 acgcgcgcaa gtttaaagca acaatcaagg tcgctgacca gggtgaagaa gggattgtgg
     2821 aagctgacag cgccgacggt agttttatgg atgaactgtt aacgctgatg gcagcacacc
     2881 gcgtgtggtc acgcattcct aagattgaca aaattccggc gtaaatatgg ataaggtcac
     2941 gtcccctggg gagtgacctt accaccttac ttacacatta agataactac ccaaccgata
     3001 accacgctca gcaattgcat atttcaacga cgctgatgtc agcacttcca gctccgttaa
     3061 ccgtgggaaa cagtaagcac tctgacggat agtattatcg ataaacgccg gatgacacat
     3121 cacctccagc gaacgatcac cccgatggct tgcgtcatcc agcacttgca ggaacagtgc
     3181 ctcgctaatc tcttcaccat aaaacgcgct actgaatccc tgagaactgc gcagattagc
     3241 cggtaaatca ccagcgttag atagcggctg acggtcgatg cgcaacgcaa tcccctcttc
     3301 agccgcaaac ctggcaacaa tcgggaaaat ctgcgggaac atatgcacat gatgatggct
     3361 atcaagatgc gtaggtttgc gtccaaatag ctcaatgaaa cgcaaatact gactggcaag
     3421 ctcctgagta atttcttcca gcggtaaagc atcttcttct gccaactgcc agatccattt
     3481 tcccagcaca ccatcgcggg ttaaccccgg catagctgtc agtggcttgc ccatagtaag
     3541 gacaaagtgc atccctatgg ccagactcgg ttcatcacga ctcaactgca ccgcatggtc
     3601 aatagcctgc ccattcacca gcgccgtcgt tgatgtgaca atcccattgc gacaggcctc
     3661 gataatgccg tagttctgac ctttgcttaa gccaaaatca tcggcattaa caatcagtaa
     3721 gcgttccata atcagcctcg gttaatgtgc ttttttaagc tctgcgatgc agtcggcaaa
     3781 atttggcagc catttctcgt gcgccagaat catctcgcgt gccagcagct cagcatcgcg
     3841 atcggaatgc accaacggac taaggtttag cgccagtaac acatcgttaa attctccgct
     3901 aagtgcggcg ttgctggcag caatctcgaa gcctttaatg gtgtgaatca gccccatcac
     3961 tttatcatcg aaatgcgtaa tgcgcggatg tggcgtcgcg ccatcgcgcc ccagcttaca
     4021 ggtcatttct accgcccagt ctgccggaat attatcaata tgcccatgat gcgggatatt
     4081 aacgtaatgt tcagcttgct tgtcgttgta gatagcgttg atcacttcgc acgctgcatc
     4141 agagtaataa gccccaccgc gctgttccag ttctttcggc ttaactttca actccggatt
     4201 tttatacagc tcaaaaagtt gtttctcgac tttctgtact acctgtgctc gtgcgccgcc
     4261 tttgtagtat tcgcccattt caatagccag catctctttc tgcttgaagt aatacagcag
     4321 ataagaacat ggcagcagat ttaacgaacg aattaagccc tcactaaatg gcagatcgaa
     4381 aatatttttt acagaggacg cttttaactg ccctgacgcc acaccatcaa gcaattcggc
     4441 aaagcgcgac ttgccattta ccagcacatc cttaatgaac accatatggt tgaggccgaa
     4501 cagatcgata gataaatcat cgctgtcttt cagcatcaga acatcgcgaa taaacatctt
     4561 catgccgatc ggaatattac acacgccgat aaagcgttta aatccggtat gacgataaac
     4621 ggcttcagtg accattcccg ccgggttagt gaagttaatc acccatgcat tcggacaaag
     4681 ttcttcgaca tcttttacga tgtcaaaaat caccggaatg gtacgcagac ctttaaacag
     4741 accgcccgcg ccgttggttt cctgaccaag ataaccatga cttaatggaa tacgttcatc
     4801 cagttcgcgc gccggtaatt ggcctacgcg cagttgggta gtaacgaaat cagcatcttt
     4861 caatgcttcg cggcgatcca gcgttttata aagcttcatc gggacgccag cgttatcaat
     4921 catccgttgg cagagttcaa aaatgatatc cagtttctct ttaccatctt cgacatccac
     4981 cagccataat tcgctgaccg gcaattcgtg ataacgctta ataaatcctt ccagtaactc
     5041 cggggtatag ctgctcccgc caccaatagt gacgactttt aatttctggc tcataatttc
     5101 tcccttcagt acagaatact gatatctggc atatctgccc ccccggacat aaataatcca
     5161 gcaacaggac agatatgtga attgtcaggt ataacgactt actgattaaa ttcagtcaat
     5221 ttcttacgat agctcttagg cgtaaaggac gttaatttct taaacgtttt aataaacaaa
     5281 ctcgggctac tataaccggc ctcaaacgca atatccgtca cggaatagtt ggtcatttcc
     5341 agttgttttt tggcaaaatt aatacggatc tcattaataa tctgcattgg tgttttgcca
     5401 taatatcgtt gagtcgctcg cgtcaaatat tcctgtgatt tggctgacaa cgtcaccata
     5461 ttctccagcg ccgattcact aaactgctct ttatcatgca tcttttctac cgtacttttc
     5521 agccactgcg gtacatcatc aatcacctgt tcttcgcggt aatgacgtaa acggttaatg
     5581 acataaaaag taaccatctc aacaaactct tccagccctg tttcgcggaa attcaatgaa
     5641 gaaatcactg tttccacata ggtgagaaaa gcgttattgg tccggtatac ctgcgaagcg
     5701 acaaagcaat aaggcaacaa cggcaggtaa tgctgctcaa aaaagcgttt actgatccca
     5761 acgttcaata tacgcgtggc accaaactca taaaaacttt gatggtgcga acctaacgga
     5821 ataaaaacaa aatcgccccg ttccagtaac acgcgcttac cgttaatctc ctggaaataa
     5881 cgcccggtta ataccagagt aaattcataa tagtcgtgct gatgcagccc gctgatactc
     5941 tcagttttgt tatagataaa cacatggaag tttttgccat taaacaactg ctgttctcgg
     6001 gcagtggcaa tttccggcgc gttaatcact ggctgcatca tcgactcctt atgccttcag
     6061 tttttcatga agctcaatta attcagtaat cagttcacgc gcaagcatgg acgtcattaa
     6121 atgatcctga gcgtggacca gcaccagact cactttcatc ttaccttcgc ccgcatcgcc
     6181 ttcaatcagt ttcgtctgta ccagatgcgc ttcattcaat gccatccgtg actgatccat
     6241 catggctttt gctgcagcaa aatcgccctg cttcgcctgt ttcagtgccg catacgccag
     6301 gctgcgcgct tgtccggagt tgatgataag ccccatcacc acttcttcca gctcttcagc
     6361 ttccgtttgc gtatcgggaa tgttatcgag atccatcata catcgttcct ctctattctt
     6421 accggcacga ttacccgtac cggcatcgat taaaatttca gggcattagc gatatcttct
     6481 tcgctctctt ctttatcaat cgcgttttgt gctttgttag ccaccacaac aaagggcaga
     6541 taaattaacg ttgcgatacc aaggttgaag agtgcgacca gcaatgcggc gacgctaccg
     6601 ttggtgttaa agaaggctcc cagaccggtt ggcatggtcc acggtgcaat attggtcacc
     6661 ggaggaatga tgcccatgta gtacgctgcg agggtgattg ccgccagaat cggttgtacc
     6721 agtacaaacg ggataaacat caccgggttc atgataattg gcagaccaaa cagaatcggt
     6781 tcgttaatct ggaagatgcc ggacggcagc gccagttttg ccacctgacg ataatcagca
     6841 cggcgagagg cgataaagat agccaggatc aggcctaaag tcgcaccact gcccccaagg
     6901 aaaataaagg agtccagcat cggcttggcc cagatatgga aggtcttacc ggctgccagc
     6961 gccgcttcga cggaaccata ttgctgatag gtcgcgatat tttccagcgc ccacggcgtc
     7021 ataatgccgt tgtccagtgc ggtcagcgcc agcgcgccat gaataccgaa gaaccagagc
     7081 agtggaacaa agatcacata ggcccagccc accacgctac ccaacgatgc cagtggggtt
     7141 gagatggtat ccataatgat ctgatggaag ttggtgcccc aggtattcaa cgcccaggca
     7201 ataatcccca tcacggaaag aataataaag ccgggaatta atgccgagaa ggaacgcgat
     7261 actgaagcag gtacgctgtc gggcagttta atgacccaat tgcggcggac aataaaggta
     7321 aacatttctg ccaccaccag gccaataata atcccggaga tgatattcgc cccacctaac
     7381 cagtttgcgc caaccgcata ggcctcaccg acactatatg gggtgacggt cataaatgct
     7441 gcaacggata acaacccagc cgccagcgca tcgactttac gctcttctgc cagcgccatg
     7501 ccaataaaga acggtgccat taaagacatt atccctaatg ttccgttata tacgttgccg
     7561 ccaatacctt tcagaccatt aagtgtttca atggttgagg cgtcgaggcg aatacctaag
     7621 gaataaaaaa acgacccctc cccaaagctt agaaaaacgt tgttaattaa tacaaacatg
     7681 gcccccgcaa gggttaacgg cattaagcga ataaagccat ttttgattgc attaacgtgt
     7741 ggctgctttc ctattttaac tgcaaaaggg aggagtacct tttcaagcga tgcaataaca
     7801 ttactcatag aaaaataccc ttaaaaaccg caatttaaat attgcggtat tgatttatga
     7861 aataactctt tgacgggaaa atttaaaata aattaatttg ctgcggcttt tttaatcgct
     7921 gcaaccgcag ccttaagcac gcctaaacca tcgactttgc cataaagcag cgagtcaatt
     7981 acttcaaccg gtttgttggg taacaaacgc tggatttcgg gcaacatata agcaatctgc
     8041 ggccctaata acacgacatc ggcattctga cctttttcac cagccagtgt ttccggaaat
     8101 gcttcaataa tgaccggaac ttcatatttt tctgcctgtg cgcgcatttt tgataccagt
     8161 aaagaggtag acatgcccgc agaacaaaac agataaatgt gtttcttttc cataaaactg
     8221 ccctcatcga cgattatctg tcagccagac actccgcaag ccttaacctg cttccatgct
     8281 ctgggtaact tgcgaaacca acatgacttt ttttgtggct gaaaggagta tacggtatag
     8341 acctgagcaa tagtatttct ttgaccttat aaattgtttc tccttgacct gtggttatga
     8401 ccctcttcac atttcgggca aataattcgc gaagataatt aagaactcag gccaaaaaaa
     8461 accggcgcaa tggccggttt ccgttgttac tcaagctttc agacgaattg attacttcgc
     8521 agcctgtgga tcagtgtcgt attcagcaca ggtctgataa ccggagttga tgacatgtcc
     8581 ggtatcatct aacgcgacaa agtaggtttc tgctttacca tcacgttgac ccaggatgta
     8641 ggtctggcaa gtaccgcgag catggatcat gctcacttca gacgaaggtt tacccgcaat
     8701 ttgtgcaacc tgcgcccggc tcatgccttt tttgacgtct ttcaccacag gctgtacaaa
     8761 ctggtcttta gtacgatcat aagccgtaca acccgccagc atggttaata ccgccgctgc
     8821 actcagaatt cctgccatat tcttgttcat attccgtcct cttgtttatc agcgtgttag
     8881 ataagcctgg aatacattgg gcgctttttc aagcccgtga acgaaacagc tccgctttca
     8941 gaggattcct gtatgacgtt ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta
     9001 gcaacgggtt agctttaagg aagttttgtc ttttctgtct ggaggggttc aatgacattg
     9061 caacaacaaa taataaaggc gctgggcgca aaaccgcaga ttaatgctga agaggaaatt
     9121 cgtcgtagtg tcgattttct gaaaagctac ctgcaaactt atccgttcat taaatcactg
     9181 gtgctcggga tcagcggcgg tcaggactcc acgcttgccg gaaagctgtg ccagatggcg
     9241 attaatgagc tgcgccagga aactggcaac gaatcactgc aatttattgc cgtacgcctg
     9301 ccctatggtg ttcaggccga cgaacaagat tgccaggatg ccattgcctt tattcaaccg
     9361 gatcgcgtat taaccgttaa tatcaagggc gcggtattgg ctagcgagca ggcattgcgg
     9421 gaagcaggca ttgaactgag cgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg
     9481 aaagcacaat atagcattgc gggtatgacc agcggtgtcg tggtgggcac cgatcatgca
     9541 gcagaagcca ttaccggatt cttcactaaa tatggtgacg gcggtacgga tattaatccg
     9601 ctgtatcgtc tcaacaaacg ccagggtaaa cagttgctga cggcattagg ttgcccggaa
     9661 cacctttata agaaagcccc aacggccgat ctggaagatg atcgcccttc tctgccagat
     9721 gaagtggcac tcggcgtgac ctatgacaat atcgacgact atctggaagg gaaaaacgta
     9781 cctgaacagg tcgccagaac aatagagaac tggtatctga aaaccgaaca taaacgccgt
     9841 ccgccaatta ccgttttcga tgatttctgg aaaaagtaat aatttgcacc tattggattg
     9901 tgcgaaaaag agtaatttgt tcacgccgga tgcggcgtga acgccttatt cgacctacaa
     9961 aactatgcaa attcaataca ttgcaggagt cgaataggcc tgacaggcgt agcacgtcag
    10021 acggtgtaac ctttgtcatc aacccgcctc ttttttaatc gcctcccgcc tgttacactg
    10081 gatagataac cagcattcgg agtcaacagt ggtacggcgt ttaacttctc cgcggctcga
    10141 atttgaagct gcggcaattt atgaatatcc cgaacattta cgttcattcc ttaatgactt
    10201 acccacccga ccaggggtgt atctgtttca tggcgaaagt gacaccatgc cgctctatat
    10261 cggcaaaagc gttaatatcc gcagtcgggt gctttctcat ttacgcaccc cggatgaagc
    10321 cgccatgcta cggcaatccc ggcgaattag ctggatctgc accgcaggcg aaatcggcgc
    10381 tctgctcctt gaagcgcgat taatcaaaga acaacagccg ctgtttaata aacggttgcg
    10441 ccgcaatcgc cagctctgtg ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc
    10501 caaagaggtg gatttttcac gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc
    10561 agctttgcaa gcattgcaga gcatcgctga tgaacaaaaa ctttgttatg gcctgttggg
    10621 actggaaccg ttaagtcgcg gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg
    10681 agcatgctgc ggtaaagaga gccatgagga acatgcgcta cgcttgcgcc agtctctgga
    10741 gcgtttgcgg gtggtgtgtt ggccttggca aggtgcggtg gcactgaaag aacagcaccc
    10801 ggaaatgact caatatcata ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct
    10861 ggaagaagcg acaacgttaa ttcggacacc cgccgggttt gatcacgacg gttataaaat
    10921 tctttgtaag ccgctgcttt ccggtaacta tgaaattact gaacttgatc cggcgaatga
    10981 ccagcgagcc agttgatttc actgaacaac agtttacctt gcggttgtaa aagccgcagg
    11041 gtgtgcctgc catcgaacca gcacgcgccc tggtcggtag tgagcaattt atcgcccagt
    11101 tgccacgccc cgttaatcac aaacaccacc ccgccgcgtg agccaaaggt agtaaacgta
    11161 cgttcggcaa ttctgacttt cgccttacag acatccaggc gtgtcatgat gttgaaatcc
    11221 atcgacatct gccctgccgt cagtttcgct tttaccacct ggtccgctgc aaaggcaaaa
    11281 ggctgtaacg gttttaaggt atggttaaag cggtctgcgc tttcaaggaa catctcgccg
    11341 ccttccagca acgtcactat cctttccatg ccgggaaaca aagaaaactc cccattcgcc
    11401 gcgatggatg caatgctggc acgccagtga aaatcacgtt ttgccggtgg gaaagtgcaa
    11461 atttcgcgcg tttcaccggc agcgtttcgc cacagattca ccgacatttt acgcatatca
    11521 aagtattcca tactcgctcc cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg
    11581 atggttatcg gcaactcggg tgatttacgt tagtggtgat caggcttttt ttacaactgt
    11641 ttgagaagag aaaagaaaac cgccgatcct gtccaccgca ttactgcaag gtagtggaca
    11701 agaccggcgg tcttaagttt tttggctgaa agattattca gcagttgcag gcattttacc
    11761 ttttgccgct ggacgttctg tcagacgctt ctcaaaatta gcattaaatt gctttttctg
    11821 ttccggcgtc aggatgttgt aaattttgtt ctgggtttcc atgtgcgcca gcatgttagc
    11881 tttgcgctgt tcttccattt ttgcgatctg cgcttcagct tttgctttat cgaaggtatc
    11941 gctggcaatg atgtcatgca ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc
    12001 acgctggcct ttcatgattt cgcggatctg ctgtttctgc gcgtcggtca ggttcaggtc
    12061 tttgaacatc atgtcctgat gcggaccgaa cttgcctttg tggtgcatca tcggcttcgc
    12121 gtcagccggt gctgcggtag tggtgtctgc ggcatgggcc aggttagccg cgccaagagc
    12181 cagggtagag gcaacaaaca gtgcagttaa tttacgcata ttctatatcc ttcctttcag
    12241 ttatttatta cggctttctt aagtagcgtg ccgtgttgac gagattaact ttactgactt
    12301 tagcgtcaat taatcagagc aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc
    12361 aattatggag aaaaaaagaa aatttgatgg agagtgatga gagaataata caacacgatg
    12421 attttgcaga gattatgaag aactataccg gatgactggt gataaataaa gcaaataacc
    12481 aggattaatc tgtattaatt tataagaaag caacttaata cccgcagaat gatttctgcg
    12541 ggtaagtatt agcttatttt ttcgagcatt aatcccgcgc gtaatcccaa cgctaccaac
    12601 ggattaggga ataacacata ctctacatca tgggttacgg taaaacgttc ctctccgtcc
    12661 tgcgccagca atgttccttt ctcaaacggc ataaaattca gcgtgtcact tgccatatgc
    12721 atttcgaagg acggcgagtg gcgagtaatt tgcgaaacca cccgataacg gagcggcggt
    12781 gttctcacga taccgacact ctcaccagat agcagcgcag caattgcgct ggcagttact
    12841 gcaaactggc gaagatcgtt ttgcccaaag ggcaacgctt tgccaagttc cagcgtacag
    12901 gccagcgcgc caaaatgtct ggcgctgaaa tgggtaaacg taccaccagg ttcctgatgg
    12961 aacaccagcg cctccagccc cgccgcaccc agccacgtca gaaatttctc gtcccaggga
    13021 atgtcgcgtt gcggtaatac accgaactgc ggatgcaagg agccacgaat tgcggtatgt
    13081 agatcaaggt gccagcgcac agattctttg cccaggtcat aaaaatcttc caggcactgt
    13141 tccagttcgc gcgcccgaca ggtttctccg ctttcagcaa atagctgcca acgaccgcca
    13201 aacattcgat tcatatcgct atggcaataa cgtttccctt gcttcagcgc aggaggattc
    13261 ccgaggatca ccagcaaccg ccaacgtaac gggatctcgc cgtgagatat cgcgccaagc
    13321 aacgcgtcca gcatctccac aggtgccgtc tcattaccgt gtattcccgc tgaaatcacc
    13381 agtgcgcctt gcggtggcgt taatggcgtc agttccagca caccatcgcc cagccagcgc
    13441 cagcgaacgc cgttgatttc tcgctcggtg ataaccggtt ttttacccgt taaggtcaga
    13501 gcaagaaaat tatccattgc cccctccctc gcgctggaac ggataaaccg aaccgagatt
    13561 cagtaattgg ctcaatacat ccagtgcttc ccgccctccg cgcagcaatt gcgggtcggc
    13621 cagatcggca gcagtaaggc gatcgcggta gtaacgatcc acccagtcat tgagcgcatt
    13681 aaacagcgta tcgttcatca tcaccgccgg attcaccgcc cggcgttctt cttctgtcaa
    13741 taccacccgc aaccgcaggc acgccgggcc gccgccattc gccatgcttt cacgtaaatc
    13801 aaagactttt agttcgctaa tcgggttgtc agcggcaagg agttcattga gataacccca
    13861 tactccggcg tgttcccgac actcctgagg cagcaccaac atcatggaac catcatcgcg
    13921 gctcaacagt tggctgttaa acagataggt agacaccgca tccgacacgg aaacctgagt
    13981 tgccggaact tctatcgcca taaaaccgtt cacccgcgca cgcaggtttg ccagtaactg
    14041 tgactggcga gcgaacgcct gttggtggca aaacagcacc tggcggttac tcacggcaat
    14101 cacgtcatta tgaaaaacgc cctggtcgat aacgtccggg ttttgctggg cgaaaatcac
    14161 ctgttgggga ttcacctgat tcagccttgc caccgcctcg ctggcttcgc gagtctgtcg
    14221 cgccggataa cgggaaggcc gggtatcatt gccttcttct cgcccgtaga caaaaagttg
    14281 catacccggc tcaccgtaat gaccgccgag acgattgtgg tttgccgccc cctcatcacc
    14341 gagcaacgct acctgcggca acgccgaatg aacgctaaat ttctcttcgt cgttaaaaat
    14401 cgcttttaaa agtgattcgg tgacgtgcgc ttccagcgag cggtgaaatt tattgttcag
    14461 gttggcaacg gtgagatgca ctttgccatc cagcgtatcg gcagatggcg cgatcgttgc
    14521 cgcattggct acccacattg gcgaagcgga actgacgctg gaaagccagt gcggtgcctg
    14581 gcgtgcaact ttttccagta cctgctcatc gctaccactg aatcccaact gacgcagcac
    14641 cggaataaac ggacgctcgt gcggcgggat cacggcctgg gggaatcccg catcggcaag
    14701 ggctttcatt ttcagtaagc cctgttttgc cgcctgtcgc gggttagaca cctgaaaacg
    14761 gtgacgggta gaggcttcat taccaaacga caggccagcg taatgatgcg tcagccctac
    14821 cagcccgtcg aaattaactt cccaggcgtt catcgcacca cctcatcgga aaaatccagc
    14881 ccggggttaa gcgttgcggg caaagttaac gagtccgact ccaggctcgc cataggccat
    14941 gcgcagtaat ctgcggcata ccaggcgctg gggcgatggt taccggatgc accaatgccg
    15001 ccgaatggcg cggtactggc agcaccagta agcggtttgt tccagttaac aatccccgcc
    15061 cgcgcctcca gcaacagttg atcgaatttt tcccgctcgg gggaaaccag accgcaagag
    15121 agtccgaagc gagtgttatt cgccattaga atcgcttcct cgaaagaatc ataacgccag
    15181 acgcgcaata acggtccgaa cacctcttca tctggtacgc cagcaacgcc tgtcatttca
    15241 atgatccccg gcgtcagcaa cgatgtctct gattgtaata agcgcggcgc aagcagggtt
    15301 cgtccgccca tcgcttccag ttgctgccag gcagtaacca cctgctgtgc ggcctgttca
    15361 gaaatcagcc cgccaataaa cggctgcggt tcgtcatccc agttgcccgg cgttaatcgc
    15421 tggctgacgg caaccagacg agcaagaaac gcatcgccct gcgctccgct tttgagcaat
    15481 aaacggcggg cgcaggtgca gcgttgcccg gctgtgacaa acgccgactg aatggtcaga
    15541 tggacagccg cgtcgatatc cgccacctca tcgataatta gcggattatt accgcccatc
    15601 tcaagggcga gaattttctc cggctgaccg gagagctggc gatgcagctg gtagccggta
    15661 ttggcgctac cggtaaacag caaaccgtcg agatcctcca gcgcactcag cgcctgaccc
    15721 gtttcacgcc cgccctgcac caggttcagt acgcccggcg gcaagccagc ctgctgccat
    15781 aaacgcatta ccgcttcgcc actccacggt gtcagttcgc tgggtttaaa gataatggtg
    15841 ttacctgcca gcaatgccgg aacgatatgt ccgttcggca aatgaccagg gaaattatac
    15901 ggcccaaaca ccgccagcac gccgtgcggg cgatgtcgca ggctcgccgc accgtccggc
    15961 atttcactac gctgctcgcc ggtacgaacg tgatacgcct taattgatat cgcgatttta
    16021 ttgatcatcg ccgtcacttc ggttgccgct tcccagcgcg gcttacccgt ttctctggca
    16081 ataatcgcgg ttaattcgcc tttattcctt tccagcaatc cggcaaagcg ttcgacaacg
    16141 acctgacgtt cagccaatga gagccgcgcc cagcgcggaa acgccgcacg ggctgcgcga
    16201 caagcctgcc cgacctgagc ggcatcggca tcattgcctt gccataacac ctcgcccgat
    16261 accggattac gcttcacacg cgatgcgccc tggcccgtta tccagtcacc gttaatccat
    16321 aaagtcatgc tgttttctcc tctgcgcaca ggcgcaccag acgaacgcga tcccctgcgt
    16381 ggcatttgag ggcatccagt tgtgcggcgg ttaaaatcaa acgctcggtt gccggatcgg
    16441 tacgcgccag caccacgcgg aaatggtgat aattttcatt ggcgaccagg caggccggaa
    16501 aatcgccctg cgcaggctgc ccttctgcca cttccaccag ccgactttta cgaatggcgc
    16561 gcacgcggtc gatgtcacac tcaagcgttg gcccgccgtc gaagatgtcg atatagttgc
    16621 ggtaacgaaa accttctttc tccagcaccg cgcgggcagg cgcggtttgc ggatgtacct
    16681 gaccgatgac gtcctgggct tcctgggata aaaagtgggt atagatcgga tgtttcggca
    16741 tcagttctgc aataaatgcc ttttgcccgg tgccgcagag aaaatcggcg cggctaaaat
    16801 ccatcgaaaa gaagcgttta ccgaggcttt gccagaacgg tgaatagccg tgttcgtcaa
    16861 tcaccccgcg catttcagca accactttgt cattaaactt gtcgcgaaaa gccgccataa
    16921 acataaagcg cgatttcgac agcaaatagc cgttgccctc tttgcgccag tccgggtcga
    16981 gaaacagcgt gcacagctcg ctgctgccgg tgtgatcgtt actgagaaac agcgtcggca
    17041 atgcgttata gacattcagc tcttttgagg cgtgaaccaa cgtgccgacg cgatagttgt
    17101 accagagatc gttcagccca accgccacct caatggcaca aatccccgcc acggtgcctg
    17161 tctcgctatc ttccagcacg aacacatagc cctgctcact tttgggcagt tcgccttgcc
    17221 aggttttgat tgccctttcg atacgcgccg aaagcgtggc ttcattggcg ggaagcgacg
    17281 tcaggccgcc gcccgttttg ctggcaagct gcatcagcgc cgagacatct gatcgctcaa
    17341 cgggacggat gaccatcatg atgaacctcg gctaacaaag tgttcgcaag cagctgcaaa
    17401 gcgatccagt ccggtcgtca cctcttcttc gctgacattg agcgcaggcg caaaacgcac
    17461 cacgttgcca cccgcaatca gtaccatcac gcctgctttc gccgcttcct gagagatctg
    17521 tttcgcttgc ccggcgtaat cggcattcag tacacagcca atcagcaaac ctaagccgcg
    17581 aacttcactg aacaaaccat agcggtgatt aatagtatta agacgctcaa caaaccagtc
    17641 gtgacgctgt ttaacgccat taagcatctc tggtgtgttg atgagctcca gcactttgcc
    17701 tgccaccgcc gaggccagcg gattaccgcc ataggtggtg ccatgagtgc caacggtcat
    17761 cacgcgggcg cactcttcgg ttgccaacaa cgcaccgacc gggaaaccgc cgcccagcgc
    17821 tttggcggta gttaacagat caggcgtcac gccgtagtgc atataggcat acagttcccc
    17881 ggtgcgcccg acgccggttt gtacttcatc aaaaatcaac agcgcattgt ggcggttaca
    17941 caattcacgc agaccttgta aaaacgcgtt gctggctggc accacaccgc cttccccctg
    18001 gatgggttcg acaatcaccg cacaggtaga gtcgtcaatc agcgcgctgg cagagttaat
    18061 atcgttatat gcagcatgac gaatatccgc cggcagtggc gcaaaatcct gtgaataggc
    18121 tggctgccca cccgcactga cagtaaacag cgtgcgacca tgaaacgcat ttttgaacgc
    18181 cacgatgccg ctcttatggc tgccgtagcg gtcgtgagcg aatttacgcg ccagttttag
    18241 cgccgcttcg ttggcttccg caccggagtt acaaaagaag acgcgatcgg caaacgtggc
    18301 gtcgatcaat tttttcgcca gtcgcagtac cggctcgttg gtgtaaccgt tgccggtatg
    18361 ccagaactta ctcgcctgtt cgttcagcgc ttcacgcagt tccggatgcg catggcccag
    18421 cgcgttcacc gcaatgccac ccgcgaagtc gatatactct ttcccctgct gatcccacaa
    18481 gcgcgaacct tcgccacgta ccggtataaa gggtgccgga gcgtaaacag gtatcatcca
    18541 ttcatcaaag ttttcacgcg taattggctg agacatagcg acctctacag taaataattc
    18601 gttatttata tgttaacaat aagtaatgtt tgcgctgtaa atgtagattg cagggttcgt
    18661 gccagccagt gataaaagtg cataaacggc ggaggctaac tggaaatcaa ggagttataa
    18721 ccaaaccata tgcatttaaa gtgcatataa agtgaatacg tttgcgatgt gggtgaataa
    18781 aaagaataaa aaacgcaatg ttatgcagaa gtaaaatata attctggaat tgtgatcatt
    18841 gacgaaattt actggaaatt actgcgccat tctgacgcag cgcgcaccaa aagcgggcat
    18901 tttttgcgcc atcgttgaca tcattaacaa ccatcgatca aatcacttaa caacaggcgg
    18961 taagcaacgc gaaattctgc taccatccac gcactcttta tctgaataaa tggcagcgac
    19021 tatgaaattt gtctctttta atatcaacgg cctgcgcgcc agacctcacc agcttgaagc
    19081 catcgtcgaa aagcaccaac cggatgtgat tggcctgcag gagacaaaag ttcatgacga
    19141 tatgtttccg ctcgaagagg tggcgaagct cggctacaac gtgttttatc acgggcagaa
    19201 aggccattat ggcgtggcgc tgctgaccaa agagacgccg attgccgtgc gtcgcggctt
    19261 tcccggtgac gacgaagagg cgcagcggcg gattattatg gcggaaatcc cctcactgct
    19321 gggtaatgtc accgtgatca acggttactt cccgcagggt gaaagccgcg accatccgat
    19381 aaaattcccg gcaaaagcgc agttttatca gaatctgcaa aactacctgg aaaccgaact
    19441 caaacgtgat aatccggtac tgattatggg cgatatgaat atcagcccta cagatctgga
    19501 tatcggcatt ggcgaagaaa accgtaagcg ctggctgcgt accggtaaat gctctttcct
    19561 gccggaagag cgcgaatgga tggacaggct gatgagctgg gggttggtcg ataccttccg
    19621 ccatgctaat ccgcaaacag cagatcgttt ctcatggttt gattaccgct caaaaggttt
    19681 tgacgataac cgtggtctgc gcatcgacct gctgctcgcc agccaaccgc tggcagaatg
    19741 ttgcgtagaa accggcatcg actatgaaat tcgcagcatg gaaaaaccgt ccgatcacgc
    19801 ccccgtctgg gcgaccttcc gccgctaatt tagcagctct cctggctcaa actgggtcag
    19861 gagaattaac cttgagaaaa atcaacaaac tgtcagtaat gatttgttgc ctgccgtcct
    19921 ttgttatacc gtcactgcgt ttttagttgt ctgaccactt ctctattatc aagtttgata
    19981 taggaaactc cacgatgaac gctgagcgta actttctgtt tgcctgtctt atttttgcgc
    20041 tggtcattta cgctatccac gctttcggtt tattcgatct gctcaccgat ttaccccact
    20101 tacagacact cattcgccag agcggattgt tcggttatag cctctatatt ctgttattca
    20161 tcattgccac cctctttctg ttaccaggaa gcatactggt gatcgctgga ggaatagttt
    20221 ttggcccgct cttagggaca ctactctcat taattgccgc cacgctggcc tcctcgagct
    20281 cattcctgct ggcgcgctgg ctgggacgtg atttactgct gaaatacgtg ggccatagcc
    20341 ataccttcca ggccattgaa aaaggcattg cgcgtaacgg tattgatttt cttattctga
    20401 cccgcttaat cccgttgttt ccttacaata ttcaaaatta cgcttacgga ttaaccacca
    20461 tcgccttctg gccttatacc cttatttcgg cactcacgac cctgcccggt attgttattt
    20521 ataccgtgat ggcaagcgat ctcgccagtg aaggcattac gctgcgcttt attttacaac
    20581 tctgtctggc gggcctggcg ctgtttattc tcgtccagct cgcaaaactc tacgcccgac
    20641 acaaacatgt ggatctgtct gcttcgcgcc gcagcccact tactcaccca aaaaatgaag
    20701 gatagaacga tgtcgcaaca ttattcagtg tcatggaaaa aaggactggc tgcactctgc
    20761 ttactggctg ttgccgggct tagcggctgc gatcaaaaag agaatgcggc ggcaaaagtg
    20821 gaatacgacg gactttcgaa cagccaaccg ctgcgcgtcg atgccaataa ccatacggta
    20881 accatgctgg tgcaaattaa tggtcgtttc ctcaccgacg tcactcgtca cggtattgtg
    20941 tttaaagatg gctccaacgg acataaatcg ctgtttatgg cttatgcgac cccgaaagca
    21001 ttttatgaag ccctgaaaga ggcaggtggt acgccgggcg aaaatatgac gatggataat
    21061 aaagaaacga ctcttgtcac aggcagcaaa ctggatattt cggtcaactg gcaaggggcg
    21121 gcaaaagcgt attccttcga tgaagtgatt gttgacagta atggcaagaa actggacatg
    21181 cgctttggcg gtaatttaac ggcagcagaa gagaagaaaa cgggttgcct ggtgtgtctg
    21241 gatagctgcc cggtcggcat cgtcagcaat gcaacataca cttatggtgc ggtggaaaaa
    21301 cgcggcgaag tgaaattcaa aggcaatgcc tcagttctcc cggcggataa cacgctggca
    21361 acggttacct ttaaaatcac cgaataaagc caggataaag gatgatgaag atacaatcgc
    21421 gtaaaatctg gtactaccgt ataaccctca tcatcctgtt gttcgctatg ctgctcgcat
    21481 gggcgctgct tcccggcgtc catgagttta tcaatcgcag cgttgcggcg tttgccgccg
    21541 tggaccaaca gggtatagaa cgctttattc agtcttacgg cgcactggcg gcagttgtct
    21601 cgttcttgtt gatgatttta caggccattg ctgcaccgct acctgcgttt ttgatcacct
    21661 ttgccaatgc gtcgctgttt ggcgcgttct ggggcggctt gctgtcgtgg accagttcga
    21721 tggccggcgc ggcgctgtgc ttttttatcg ccagagtgat gggccgcgaa gtggtggaaa
    21781 aattaaccgg caaaaccgtg cttgacagta tggacggctt tttcactcgc tacggcaaac
    21841 acaccatcct ggtctgtcgg ttattgcctt ttgtcccttt cgatccaatc agctatgctg
    21901 ccggtctgac ttcaatacgt tttcgctcgt tttttatcgc caccgggctt ggtcaattac
    21961 cggcgactat tgtttattcc tgggcgggca gcatgttaac aggcggtact ttctggtttg
    22021 tcaccggact gtttattctg tttgccctga ccgtggtgat ttttatggcg aagaaaatat
    22081 ggcttgagcg ccagaagagg aatgactgat gggtttaccg ccgcttagca aaattccttt
    22141 cattttacgt ccgcaggcgt ggctgcatcg tcgccattac ggcgaggtgc taagcccgat
    22201 tcgctggtgg gggcggatcc cgtttatctt ttatttggtg tcaatgtttg ttgggtggct
    22261 ggagcgcaaa cgctcaccgc tcgatccggt agtgcgatcg cttgtcagcg cgcgcattgc
    22321 gcaaatgtgc ctgtgtgagt tttgcgtgga tatcaccagt atgaaagtcg ccgagcgtac
    22381 cggcagcaac gataaactgc tggcagtggc tgactggcgg caaagcccgc tctttagcga
    22441 tgaagaacgg ctggcgctgg agtacgccga agccgcaagc gtcacaccgc caacagtcga
    22501 tgatgccctg cgtacccgac tggctgcgca ttttgacgct caggcgctca ccgaactgac
    22561 ggcattgatc ggcctgcaaa acctgtcggc tcgttttaat tctgccatgg acattcccgc
    22621 tcaggggctg tgccgtattc cggaaaaacg ttcttaagga gggaagatgc gccattgtgg
    22681 gtggttgctg ggattgttat cgctgttttc tttggcaaca catgccagtg actggcagga
    22741 aattaaaaat gaggccaaag ggcaaaccgt ctggtttaac gcctggggcg gcgataccgc
    22801 aattaaccgc tatctcgact gggttagcgg cgagatgaaa acccattacg ctataaacct
    22861 gaagattgtt cgtctcgcgg atgccgcaga cgcggtgaag cgcattcaga ccgaagctgc
    22921 tgccggacgt aaaacgggcg gctcggtgga tctgctctgg gtgaacggcg aaaacttccg
    22981 caccttaaaa gaggccaatt tattacaaac gggctgggcg gagactctgc ccaactggcg
    23041 ctatgtcgac acacagctgc cggtgcggga agatttttca gtgccgacac aaggtgcgga
    23101 atcgccctgg ggcggcgcac aactgacgtt tatcgcccgc cgcgatgtta cgccacagcc
    23161 accacaaacg ccgcaagcct tactggagtt tgctaaagtc aatcccggca cggttactta
    23221 tccgcgccca ccggacttta ccggcacggc gtttcttgaa cagttgctga ttatgctgac
    23281 gccagatccc gccgcattaa aagaagcgcc ggacgatgcg actttcgccc gtgtcactgc
    23341 tcccttgtgg caatatcttg atgcgctaca tccgtatttg tggcgcgaag gaaaggattt
    23401 cccgccttcg cccgcgcgga tggatgctct gctgaaagcc ggcacattgc gcctgtcgct
    23461 gacctttagc cccgcgcatg cgcagcaaaa aatcgccagc ggcgatttgc cggcaagcag
    23521 ttacagtttt ggctttcgcg aggggatgat aggcaacgtg catttcgtca ccattccagc
    23581 caacgcgaat gccagtgctg cggcgaaggt agttgccaat ttcctgctct cacccgatgc
    23641 gcaactgcgt aaagcagatc ccgttgtctg gggcgatcct tctgttctcg atccgcaaaa
    23701 actgcctgac gggcagcgcg aaatattgca atcaagaatg ccgcaggatc tgccgccggt
    23761 actggctgaa ccgcacgcag gatgggtgaa tgcactggaa caagaatggt tacgccgtta
    23821 cggtacgcat taatcttttt gctgtgggcg atggtggcgg tgatttatgc accgctgatc
    23881 ccggcagctc tcacgctgat atcgcctgcg ttgtcgttga cacactggca ggcgttattt
    23941 gccgatccac agttaccgca tgcattactg gcaacgctgg tgtcgacaac catcgcggcg
    24001 gtcggggcat tgttgattgc cctgctggtg attgtggcgc tatggcctgg gccgaaatgg
    24061 cagcgtatgt gcgcccgtct gccgtggctg ctcgccattc cccatgtggc ttttgccacc
    24121 agcgccctgc tgctctttgc tgacggaggg ctgctatatg actatttccc gtatttcact
    24181 ccgccaatgg accgatttgg catcggtctg ggcctcaccc ttgcagtgaa agaaagcgca
    24241 tttctgctgt ggatcttagc ggcagtattg agcgaaaaac ggctgttgca gcaggtcatt
    24301 gtgctggatt cgctgggcta cagccgctgg caatgcctga actggctgct gttgccctcc
    24361 gtcgcgcctt cgctggcaat ggcgatgctg gcgattgttg cctggtcgct ttcggtcgtg
    24421 gatgtggcga ttattcttgg gccaggtaat ccaccgacgc tggcagtaat tagctggcag
    24481 tggttaactc agggcgacgc ggatcaacaa acgaaaggcg cactcgccag tctgctgctg
    24541 atgctgttac tcgccgccta cgttttgctg agctatctgc tatggcgcag ctggcggcgc
    24601 actattcccc gcgtagatgg cattcgcaag cctgccacac ctttattacc aggcactacg
    24661 ctggcgagtt ttttaccctt aaccggtgtg ctgtgtgtgg ttctgctggc gatcctcgcg
    24721 gatcagtcga cgatcaatag tgaagcactc atcaacagcc tgacaatggg gctggtggcg
    24781 acattcatcg ctttgctcct gttactgctg tggctggaat gggggccaca gcgtcgccag
    24841 ttgtggctct ggttgcccat tctattgccc gctctgccac tggtggcggg ccagtacacg
    24901 ctggcgctat ggctgaacct ggatggaagc tggacggcgg tggtctgggg gcatctgctg
    24961 tgggtaatgc cgtggatgct gtttatcctg caacccgcct ggcagcgcat tgattcacgg
    25021 ttaattttga ttgcgcaaac actgggctgg tcgcgggcca aaatcttctt ttacgtgaaa
    25081 tgcccactca tgttgcgccc tgtgctgatt gccttcgcgg tgggattttc agtcagtatt
    25141 gcgcagtata tgccgacgct gtggctgggc gcggggcgtt ttccgacgct caccactgag
    25201 gcggtggcat taagcagcgg cggcagcaac ggtattctcg ccgcccaggc tttatggcaa
    25261 ctgttgttac cgcttattat ttttgccctg accgcgttag tcgcaaaatg ggtaggttat
    25321 gtcagacaag gactccgcta atgctctgcg tgaaaaatgt ttcgctacgt ttaccagaaa
    25381 gccgcttgct gacaaacgtt aactttacgg tggataaagg tgacattgtc acgttaatgg
    25441 ggccgtctgg ctgtggaaaa tccactctgt tttcatggat gattggtgca ctggctggac
    25501 agttttcttg tacaggtgag ctatggttca atgagcaacg gattgacatg ctacccaccg
    25561 cacagcgtca gattggcatt cttttccagg atgcactgtt atttgaccag ttcagcgtcg
    25621 ggcaaaattt actgctggcg ctaccgtcga cgctcaaggg gactgccagg cgcaatgccg
    25681 tgaaagacgc gcttgatcga gcgggactgg ctgaaactta tcaccaggac cctgcgacat
    25741 tatcaggcgg tcagcgagcg cgggtggccc tgctgcgcgc ccttctcgct cagccaaaag
    25801 cgttactcct ggatgagcca ttcagccgtc ttgatgtggc tctgcgcgat aattttcgcc
    25861 agtgggtgtt cagcgaagtt cgcgaactgg cgatccccgt cgttcaggta acgcacgatc
    25921 tccaggatgt tcctgctgat agttctgttc tggatatggc gcagtggtca gaaaattaca
    25981 acaaactgcg ataacgcaaa gtttttctca atgcgtcagt tcagaatggc gcactcaaaa
    26041 ctacaatgtc gggattttcg atgaaacgtg tttctcaaat gaccgcgctg gcaatggctt
    26101 tagggctggc ttgcgcttct tcgtgggccg ctgaactggc gaagcctctt acacttgacc
    26161 agcttcaaca acaaaatggc aaagcgatag atactcgccc cagcgcgttt tataacggct
    26221 ggccacaaac cttaaatggc ccttctggtc atgaacctgc cgccttaaac ctctctgcca
    26281 gctggctcga caaaatgagc accgaacagc tctatgagtg gatcaagcaa cataatctga
    26341 aaaccgatgc tccggtggcg ctgtacggca atgacaaaga tgtcgacgcc gtcaaaacgc
    26401 gtctgcaaaa agcaggtttt acgcatatct ccattctgag tgacgcgcta agcgaacctt
    26461 cccgtctaca aaaactgccg cactttgagc agctggttta tccgcaatgg ctgcacgacc
    26521 tgcaacaagg taaagaggtt acggcgaaac ctgccggtga ctggaaagtc attgaagcgg
    26581 cctggggcgc tcctaagctt taccttatca gccatattcc tggcgctgac tacatcgata
    26641 ccaacgaagt ggaaagcgaa ccgctgtgga acaaagtttc tgatgaacaa ctaaaagcga
    26701 tgctggcaaa acacggcatt cgccatgaca ccacggtcat tctgtacggg cgtgacgtat
    26761 acgctggagc gcgtgtggcg caaattatgc tctacgcagg cgtgaaagat gtgcgtctgc
    26821 tggatggcgg ctggcaaacc tggtccgacg cgggactgcc tgttgagcga ggaacgccac
    26881 cgaaagtgaa agcggaaccg gatttcggcg tgaagatccc ggcacaaccg cagttgatgc
    26941 tcgatatgga acaggcccgt ggactgctgc atcgccagga tgcatcgctg gtgagcattc
    27001 gttcgtggcc agaatttatc ggtacgacca gcggttacag ctatattaaa ccaaaaggtg
    27061 aaatagccgg agcacgttgg ggacacgctg gtagcgactc gacgcatatg gaagattttc
    27121 ataacccgga tggcaccatg cgcagcgccg atgatattac cgctatgtgg aaagcgtgga
    27181 atatcaaacc agagcagcaa gtttcattct actgcggcac cggctggcgc gcgtccgaaa
    27241 cctttatgta cgcacgcgcc atgggttgga agaatgtttc cgtgtatgac ggcggctggt
    27301 acgaatggag cagcgatcca aaaaatccgg tagcaaccgg tgaacgcggc ccggacagta
    27361 gcaaataaca ttgcaattac tgacgctgga gcgacttcag cgtcagatac ccactccaca
    27421 cccgcgtaaa ggtcgtcatc cagcacaacg caccgaagat ccacgcaaac cacgcaaacc
    27481 atgcagggaa taaacatccc agcacaaaca gtaagatcgt ttctgtgcct tcggttaatc
    27541 cccccagata gtaaaacgac ttatgtgcat aaccggggtt atcaatctga tgttttgctg
    27601 ccagtgcagc aaaggcgaga aaactgctgc ccgtgccgat aaacgcaaac aacaaccagc
    27661 cgcctgccag cgcattttgc tccggtgcag caagaataaa gccaaacggc accagcgcgt
    27721 aaaagagaaa atcgagagaa atatcaagaa agccgcccgc gtcggtaagc tctctgcgcc
    27781 gcgccagcgc accgtccaga ccatcaagca gcctgttcaa caaaatgacg actagcgccg
    27841 ccagatacca gcccagcgcc agaaacggca gagccagcac gccgatggca aatcccacta
    27901 atgttaaacc atctggcgta atgcccggtt tatcaagaac ccgcacgcac tgatgcaaca
    27961 acggtttaat ccggggatga agatggcggt ctagcacagg cactccttaa atataaagcc
    28021 tttctgattg agcaacagtg cggatattat ggcatttttc gcttatctgc ccgtgtgtaa
    28081 tttatgaaaa tgattgaagt tgttgccgcc atcattgaac gtgatggcaa aattttactc
    28141 gcgcaacgcc ccgcccagag cgatcaggcg ggattatggg agtttgccgg tggtaaagtc
    28201 gagctggatg aaagccagca gcaggcactg gtgcgtgagt taaacgaaga attgggcatc
    28261 gaagcaactg tgggtgagta tgttgccagc catcagcgag aagtttcggg ccggattatc
    28321 catcttcatg cctggcacgt acccgacttc cacgggacgt tacaggcaca tgaacatcag
    28381 gcgctggtct ggtgctcacc tgaagaggcg ctgcaatatc cgctggcccc tgctgacatt
    28441 ccactattag aggcgtttat ggctttacgc gccgccagag cagcggatta gtgctaaggg
    28501 ttttgtcatc ccgctggcat tgcagcagta ttccttcggc tttaattacc gccccttcag
    28561 aataattttg atcctgataa acgcagcact gagtacaggg ctgcgctgac tgcccgcctg
    28621 aactgaacac ttctggcggt acgtttacct ccacgtccgg acgataatgc gggttagcca
    28681 gtgcaattaa tggaaatgct aatactacgg cgaacaatgc tcgactcaca gggaactcct
    28741 taacgttatt gtctctgcta ctgataacgg tagccgggtg gcaaaacttt agcgtctgag
    28801 ttatcgcatt tggttatgag attactctcg ttattaattt gctttcctgg gtcatttttt
    28861 tcttgcttac cgtcacattc ttgatggtat agtcgaaaac tgcaaaagca catcacataa
    28921 acaacataag cacaatcgta ttaatatata agggttttat atctatggat cagacatatt
    28981 ctctggagtc attcctcaac catgtccaaa agcgcgaccc gaatcaaacc gagttcgcgc
    29041 aagccgttcg tgaagtaatg accacactct ggccttttct tgaacaaaat ccaaaatatc
    29101 gccagatgtc attactggag cgtctggttg aaccggagcg cgtgatccag tttcgcgtgg
    29161 tatgggttga tgatcgcaat caggtacagg tcaaccgtgc atggcgtgtg cagttcagct
    29221 ctgccatcgg cccgtacaaa ggcggtatgc gcttccatcc gtcagttaac ctctccattc
    29281 tcaaattcct cggcttcgaa caaaccttca aaaatgccct gactaccctg ccgatgggcg
    29341 gtggtaaagg cggtagcgat tttgacccga aaggtaaaag cgaaggcgaa gtgatgcgtt
    29401 tctgccaggc gctgatgacc gagctttatc gtcatctggg cgcggatacc gacgttccgg
    29461 caggtgatat cggcgttggt ggtcgtgaag tcggctttat ggcggggatg atgaaaaagc
    29521 tctccaacaa taccgcctgc gtcttcaccg gtaagggcct ttcatttggc ggtagtctta
    29581 ttcgcccgga agctaccggc tacggtctgg tttatttcac agaagcaatg ttaaaacgcc
    29641 acggtatggg ttttgaaggg atgcgcgttt ccgtttctgg ctccggcaac gtcgcccagt
    29701 acgctatcga aaaagcgatg gaatttggtg ctcgtgtgat cactgcgtca gactccagcg
    29761 gcactgtagt tgatgaaagc ggattcacga aagagaaact ggcacgtctt atcgaaatca
    29821 aagccagccg cgatggtcga gtggcagatt acgccaaaga atttggtctg gtctatctcg
    29881 aaggccaaca gccgtggtct gtaccggttg atatcgccct gccttgcgcc acccagaacg
    29941 aactggatgt tgacgccgcg catcagctta tcgccaatgg cgttaaagcc gtcgctgaag
    30001 gggcaaatat gccgaccacc atcgaagcga ctgaactgtt ccagcaggca ggcgtactgt
    30061 ttgcaccggg taaagcggct aatgctggcg gtgtagcaac gtcgggcctg gaaatggcac
    30121 aaaacgctgc gcgcctgggc tggaaagccg agaaagttga cgcacgtttg catcacatca
    30181 tgctggatat ccaccatgcc tgtgttgagc atggtggtga aggtgagcaa accaactacg
    30241 tgcagggcgc gaacattgcc ggttttgtga aggttgccga tgcgatgctg gcgcagggtg
    30301 tgatttaagt tgtaaatgcc tgatggcgct acgcttatca ggcatacaaa tgggcacaat
    30361 tcattgcagt tacgctctaa tgtaggccgg gcaagcgcag cgcccccggc aaaatttcag
    30421 gcgtttatga gtatttaacg gatgatgctc cccagggaac atttcttatg ggccaacggc
    30481 atttcttact gtagtgctcc caaaactgct tgtcgtaacg ataacacgct tcaagttcag
    30541 catccgttaa ctttctgcga tagcagcaga tatgcctgta aagaaatccc attagactat
    30601 ttttttgata atcttcttcg ctttcgaaca actcgtgcgc ctttcgagaa gcaagcatta
    30661 tataatgcca ggccagttct tctccaattg tcccgttttg aaaagctgtg cttgatatcg
    30721 agatcatcca tgataattcc gccgcccata ttagcttcgc cgaggattta ccggagcgat
    30781 tattagcgca atcagagata tagtctgagg gaaaaacagc aaatttattc aacaaggcga
    30841 taacctgctc tggggcttcc tccatgtttg ctttaaaggt attggctcca tggtcgccag
    30901 aaagaaaatg ctccattaag gcacaataac tttcgctatc ttcgataccc cattgatcct
    30961 ctaaagactc gcgtctttta cttatgatat cgatcgagtc aaaaggaagc acatgatatt
    31021 ggaaggtatc tttgccaggt tcaggctttc gcggccagaa ctccagcgtt tcagaccatt
    31081 gcttatgata gaatcgataa ggtgcgatca attgtagcgc ctgtaacttc tcgatactga
    31141 gcggctcaat acctttagcc tgataataat gcagttgttc tttttttgct ttaaaaccgg
    31201 cccgaacaat aagccccatc ataattaata gataaagaaa agagcatccc gcagtaatca
    31261 ggcctctttc attcaaaccg ttggatgtta tcgctgcgaa tacaaacatt acagcgacaa
    31321 cacatgttaa ataccccccc ccacttacaa agcagcatgg ccttattttc tttaatcatc
    31381 cgttcaaaat tactattaaa tatttcccag ccattaaaag aatacttctc gctcccagga
    31441 tggttttgta ataaaacttt tttcatcaca ttccctgtta ttacatactt tagaggcgat
    31501 aattttatcc atgcaaaaaa aatatccaac gaattttctt gatccgctgg atatttcaga
    31561 atatgactcg aatagcacga aagattcgct cgcttacgct atcgccccgc ttccggcttc
    31621 atctgctggc ggactttttt tcgcactacg tttacgcggt gcagcctttt tcttatcagc
    31681 actgccacca ctgcccggag ccacaatgcc gcgaaactgc cgcaccggcg tacgtttggc
    31741 ttgatcaata agctgatata gcgtccccac cagcggctgc ataaagtcct gatagcgaca
    31801 ctgcttttcg ctgatttgcg tcagcaccga ttcccagtgc gcggtcatgt ccggtcgcgt
    31861 agccatttct ggcagcgaat ggaataacgc ttttccggcg tcggtggagt ggatatagcg
    31921 cccttttttg gtgaggaaac cacgcttgaa caacagttca ataatcccgg cacgcgttgc
    31981 ttctgtcccc agaccatcgg tcgcacgaag gatctttttc agatctttgt cctgcacaaa
    32041 gcgcgcaatc ccggtcatcg ccgaaagcag tgttgcatcg gtaaaatggc gcggcggctg
    32101 ggtttgccgc tctaccactt cacctttttc acacagcaac tcatcgcctt tcgccaccac
    32161 aggcaatggc gtgccgtcgt tttcttcatc gcgctctttg ctgcctaaca gcgcgcgcca
    32221 acctgcttca gcaagaaaac gcgctttagc gacaaatttg cctttggcaa tgtccagttc
    32281 gataacacac ttgcggaaca ctgcatccgg gcagaactgc atcagatact gacgggcaat
    32341 caggttatag accttcgctt cgttctccgt caggttgatc gcagaactcc ttgcggtcgg
    32401 aatgatggcg tggtgcgcat cgaccttttt gtcatcccaa cagcggttgc gtatatctgg
    32461 atctaccact ggctgcggca acagatctgg tgcatgaacg ctgatggcat tcatcaccgc
    32521 gtggcgtccg gcaaaatgtt cttctggcaa atagcgacaa tcggaacgcg gataagtgat
    32581 taacttgtgt gtttcgtaca gcttctggca gatatcaagc acgttctgcg cgctcagacc
    32641 aaagcgtttt gccgcttcaa tctgcaacgc cgaaagcgaa aacggcagcg gcgcggattc
    32701 tgattcccgt ttatcgttat agctggtgac aatagccggt tgaccactaa tgcggttaac
    32761 cacatgctcc gccagtggac gatgtaacaa gcgcccttct tcatcctggt acggttcaca
    32821 cgcttcgctc ggttgccaga tagcggtaaa ccgctcatcg gcaggtgtca cgatatgcgc
    32881 tttgacttca aagaagtctt tcgccacgaa gttttcaatc tcttcatcgc ggcgcaccac
    32941 cagcccgagc acgggtgtct gcacgcgtcc cacggaaagt acgccctgat aaccggcatt
    33001 gcgaccgaga atggtatacg cacgggtcat attgatgccg tacagccagt cggcacgcgc
    33061 tcgcgccagc gcagaaacgc acaatggcac aaactcactg ttggaacgaa gacggtcgat
    33121 cgcccgctca accgcctgcg ggttcaggtc gtttatcaag caacgctgca cctgctggcg
    33181 cttttccggt gccagttgca gatagtccag cacttcatcc accagcaatt gcccttcacg
    33241 atccgggtcc ccggcgtgaa cgatttcgct ggcttcatgc aggaagcgtt tgatgacgtt
    33301 aagttgtttg gtcacggagg gtcggggctg taattgccac ttttccggga caatcggcaa
    33361 atccgcaaga ttccagcgcg catagcggct gtcgtaggcg tctggctgcg cctgctcaag
    33421 caggtgaccg atacaccagg tcaccacctg accattaccg cactcgataa agccatcgcc
    33481 tttacggtgc ggtttgggca ggacatcagc aatggcgcgc gccagactcg gtttttcggc
    33541 aataaacaac cgcattgaat taacggatct caaccatggc acgaccgccg cgcgctggca
    33601 ccagttcgcc aatcgccgtc agttcaatgc caaactcggc ggctgtagtt ttaacctcat
    33661 tttctgcttc cggcatgacc gccagcagca aaccgccaga agtttgcgga tcgcacagca
    33721 gatcgcgcac ttcacgcggc atttcaccca tcagatgacc gtagctggca aagttacgtt
    33781 cggtgccgcc aggcactgcg cccaacttaa tgtactcttc aacccccggc aattttggga
    33841 tcgcgtcata gtcaacgcgc gcctgcacac ctgcgccctg acacatttcg ctcaaatggc
    33901 ccagcagacc aaaaccagta acgtcggtca tcgcttttac gccttcgatg ttggcaaagg
    33961 acgcacctgc gatgttcatc cggcacatca cttccgtcgc cagtccctga tgttctggtt
    34021 tcaacagtga ttttttctca gccgtggtaa gaacgccgat ccccagcggt ttcgtcagga
    34081 acagtttgca tccggcttgt gcggtactgt ttttcttcac ccgctcggtc ggtacgatcc
    34141 ccgttaccgc cagaccaaaa atcggctccg gcgcatcgat ggagtgaccc ccagccagcg
    34201 caatacccgc ctgacggcat gcatagcgtc caccttcggt tacttcgcgg gcaatttctg
    34261 gggaaagttt gttaatcggc cagccgagga tcgcaatcgc cataatcggt ttgcccccca
    34321 tcgcgaagat atcgctgatg gcgttagtcg ccgcaatgcg gccaaaatcg aaaggattat
    34381 caacgatcgg cataaagaag tcggtggtac tgataacgct ggtgccattg cccagatcgt
    34441 acaccgccgc atcgtcgcgg gtctcattac ccacaagcaa attcggatca acaaacttcg
    34501 cctgctcact gtgcaggatg gtttccaaca cttttgggga aattttacag ccgcaaccag
    34561 ctccgtggct gtattgggtc aaacgaatcg agttctcgct catggacatc tcctgtcaat
    34621 gcaatccggg tatggtaacc ctcattccgt gaagtgataa gtgagagtgt ctgaattcct
    34681 gcgcctttgc tcacaatcca gacagtttcg cgacaattat cagaaataag ttacaaacgg
    34741 cgtcgggtcc gggacgttaa tcgacgtaga tgctttcaac tgcggcgtgc cgaggtagag
    34801 aaaaccgaca attttatcct gctcacggca accgaatgct tcacgcacta ccggactttc
    34861 agttaatgcg ccactgcgcc agatgccgcc aaatccttgg gcaacggctg ccatttgcat
    34921 cgccatgacc gcgcatccgg cagacatttc ctgttcccat cgcgggactt tatgattttc
    34981 ttcgcatttc gccaccaccg tgatgatgag cggtgcgcgg aacggcgcat tacgggcttt
    35041 gtcgatagct ttgtcatcac taccggcagc aatcgccccc tgttccagta cggcgctgaa
    35101 acgctcgcgc ccttcccctt caatcacaaa aaaatgccac ggttgcatgg acttatggtc
    35161 cggcgcacgc atacccgcac gcaggatgtt ttgcagttgt tcacccgttg gcgcgggttc
    35221 agccaggcgg gaggcgctac ggcgattgat caatagttcg agtgcatcca tttgattaac
    35281 tcctgtcgtg atatttattc acaaaattaa cacgagagtg gattttgtta cagcacagtc
    35341 cgcaattcct gctgacaagt gccggttggg tcattacgat aaccacatct attgcgcctg
    35401 tgacaggtgt gaccttaagt tgggagaata catgcgaacc ctttggcgat ttattgccgg
    35461 attttttaaa tggacgtggc gtctgctgaa tttcgtccgt gaaatggtac ttaacctgtt
    35521 ctttattttc ctcgtactgg ttggtgtggg gatctggatg caggtcagtg gtggtgattc
    35581 gaaagaaacg gccagtcgtg gcgcactgct gctggacatt tctggtgtga tcgtcgataa
    35641 acccgacagt tctcagcggt ttagtaaatt aagccgccag ctgcttggtg ccagttccga
    35701 tcgtctgcag gaaaactcac tgtttgatat cgtcaacacc attcgccagg cgaaggacga
    35761 ccgcaatatc accggtatcg tgatggatct gaaaaacttc gcaggcggcg accagccgtc
    35821 tatgcagtac atcggcaaag ccctgaaaga gtttcgtgac agcgggaaac cggtttatgc
    35881 cgttggcgag aactacagcc aggggcaata ttatctcgcc agtttcgcca ataaaatttg
    35941 gctgtcaccg caaggcgtgg ttgatctgca cggttttgct accaacggtc tgtactacaa
    36001 atcgttgctg gataagctga aagtttccac ccatgtgttc cgcgtgggta cgtataaatc
    36061 tgccgttgaa ccgtttattc gtgatgatat gtcaccggca gcccgcgaag ctgacagccg
    36121 ctggattggt gagctgtggc aaaactatct gaatactgtt gccgctaacc ggcagatccc
    36181 tgctcagcag gtattccctg gcgcgcaagg gttgcttgag ggtttaacca aaaccggtgg
    36241 cgataccgcg aaatatgcac tggaaaacaa gctggtcgat gcactggcat cgagtgccga
    36301 aatcgaaaaa gctctgacca aagagttcgg ctggagtaag actgataaaa attatcgcgc
    36361 catcagttat tacgattacg cattgaaaac gccggcagat accggtgaca gcatcggtgt
    36421 cgtcttcgct aatggcgcaa ttatggatgg cgaggaaact caggggaatg ttggcggtga
    36481 taccactgcg gcacaaatcc gcgacgctcg ccttgacccg aaagtgaaag cgattgtcct
    36541 gcgtgttaat agcccaggcg gcagcgttac cgcgtctgaa gtgattcgcg ctgaactggc
    36601 agcagcccgg gcagcgggta agcctgtggt tgtatcgatg ggcggcatgg cggcatctgg
    36661 tggttactgg atttccacgc cagctaatta cattgtggct aaccccagca ccctgaccgg
    36721 ttctatcggt atcttcggcg tgatcaccac cgtagaaaat agtctggatt cgattggtgt
    36781 tcatactgat ggtgtctcaa cttcaccgct ggcggatgtt tctatcacca gggcactgcc
    36841 gccggaagcg cagcagatga tgcaattaag cattgagaat ggctataaac gctttatcac
    36901 gctggttgct gatgcgcgtc attcgacgcc ggagcaaatt gataaaatcg cccagggcca
    36961 cgtctggacc ggtcaggatg caaaagctaa cgggctggtc gatagtctcg gggatttcga
    37021 tgatgcggtt gccaaagcag cagagctggc aaaagtgaaa cagtggcatc tggaatacta
    37081 cgttgatgaa ccgaccttct tcgacaaagt gatggacaac atgtctggtt ctgtccgggc
    37141 aatgttgcca gatgcgttcc aggccatgtt acctgcaccg cttgcctcgg tagcctctac
    37201 cgttaaaagt gaaagcgaca agctggccgc gtttaacgac ccacaaaacc gttatgcgtt
    37261 ttgcctgacc tgcgccaacg tgcgttaagt cttgtactga gtggccgaca gatcgtcggc
    37321 cacattattt tttacctcga cgaatcctct tcccgctgtc tcgacccata tttccttatc
    37381 cgcatagtat caggtgcgct cctcctgcct cacgtatata cttttgctct ttcgatatca
    37441 ttcatatcaa tatcatgcaa aagaaatcaa tttacgtcgc ctacacgggc gggaccatcg
    37501 ggatgcagcg ttccgagcag ggttatattc cggtgtcagg tcatctacaa cgccaactgg
    37561 cgctgatgcc ggaattccat cgcccggaga tgccagattt caccattcat gaatatacgc
    37621 cgttgatgga ttcttctgat atgacgccgg aagactggca gcatattgct gaagatatta
    37681 aagcgcacta tgacgactat gatggttttg tcattctaca cggtaccgac acgatggcgt
    37741 ataccgcctc tgcgctgtcg ttcatgctcg agaatctcgg taaaccggtc attgtgacag
    37801 ggtcacaaat cccgctggct gagttacgct ctgacggaca aattaatctg ctgaatgcgt
    37861 tgtacgttgc ggcgaattat ccgatcaacg aagtaacgct ctttttcaat aaccgattgt
    37921 atcgcggcaa ccgcactacc aaagcccatg ccgatggttt tgatgcgttt gcctctccaa
    37981 accttcctcc gttactggaa gctggtatcc atattcgtcg tttgaatacg ccacccgccc
    38041 cgcacggtga aggggaatta atcgttcatc caatcacgcc acaaccaatt ggcgtagtga
    38101 cgatttatcc ggggatttct gctgatgtcg tgcgcaattt tctgcgccaa ccggtgaaag
    38161 cattgattct gcgctcctat ggcgtgggta atgcgccaca aaacaaagcc ttcctgcagg
    38221 aattacaaga agccagcgat cgcggtattg tggtggttaa cctgacacaa tgtatgtccg
    38281 gtaaagtgaa catgggtggt tatgccaccg gtaacgccct cgcccatgcc ggcgtaattg
    38341 gcggtgcaga tatgactgta gaagctacgc taaccaaact gcattacctg ctgagccagg
    38401 aactggatac tgaaaccatt tgcaaggcca tgagccaaaa cctgcgcggc gaactgacgc
    38461 cggatgatta aggagactgc aatgccccct cgcgccctgt tactggtcga tttacaaaat
    38521 gatttctgtg ctggtggcgc gctcgccgtg ccggaaggtg acagtacggt ggatgtcgct
    38581 aaccgcctta ttgactggtg ccagtcgcgc ggtgaagcgg ttatcgccag tcaggactgg
    38641 cacccggcga atcacggcag ttttgccagt cagcactgtg tagagcctta tatgccaggt
    38701 caactcgacg gtttgccaca aaccttctgg ccagatcact gtgtgcagaa cagtgaaggc
    38761 gcacaattac atccgttact gaaccaaaaa gcgatcgcag cggtgttcca taaaggcgaa
    38821 aatcctttag ttgacagtta cagtgccttt tttgataacg gccgtcggca gaaaacctct
    38881 ctcgatgact ggttacgcga tcatgaaatc gatgaattga tcgttatggg cctggctact
    38941 gactattgcg tgaagtttac cgtgctggac gcgttacagt taggttataa agtaaacgtg
    39001 attaccgatg gttgtcgtgg cgtgaatatc cagccccagg acagtgcgca cgcgtttatg
    39061 gagatgtcag cagctggggc aacgctatat acgctggcag actgggaaga gacgcagggg
    39121 taatattagt tgaattcatc tctggcctac atttcctcaa tgacattgta ggccagataa
    39181 tacttgtcag tgttgcattt aacctacttg cagctaaaac acgacgtcct aacaagtata
    39241 tttgccctcc cttaataaca ggcttaaata ttagataact cattcactta aacatagcaa
    39301 taggctttgc tgagatacca aaatcttctt ttaactgctg tttgctcttc atgaccatct
    39361 gaccatttgt gtcaattgtc atatgctgtg cttcggtatt atggcgggct tgccacagca
    39421 tcaccagttg caggcaattc tctttttgtt cctcggtcaa cgcaacgcca tcaggccatt
    39481 tccccagttc aacggcggtc gacaaacgct ggtatacctc aggcgtcatg ctgttgataa
    39541 tgtcatcaag attcataatt tttccgctcc tgtggaataa gttgctgaat cgttttttca
    39601 accgttgatt tcttcgccgt tttcgccatc ggtaaagcgt aaagaggcag agttaacaca
    39661 ataacgttcg cccgttggct gcggcccgtc agggaagaca tgccccagat gggcatcaca
    39721 gttaccgcaa cgaatttcta tgcgctgcat tccatgtgac aagtctttga tataacgaat
    39781 ggattcttca ctcaccggtt cgtagaaact gggccaacca cagccggaat catacttggt
    39841 ttgggaatga aacagcgggg catcgcagat caaacagtga tatacgccgt cacgcttgtt
    39901 atgcagtaaa cgacccgtaa atggcggctc tgtcccatga ttctgcgtca cgtaaaactg
    39961 catctcggac aaattttttt tcagttcttc tgccgaaggt ttattagcca tttgctcaca
    40021 tctcacttta atcgtgctca cattacgtga ctgattctaa caaaacatta acaccaactg
    40081 gcaaaatttt gtcctaaact tgatctcgac gaaatggctg cacctaaatc gtgatgaaaa
    40141 tcacattttt atcgtaattg ccctttaaaa ttcggggcgc cgaccccatg tggtctcaag
    40201 cccaaaggaa gagtgaggcg agtcagtcgc gtaatgctta ggcacaggat tgatttgtcg
    40261 caatgattga cacgattccg cttgacgctg cgtaaggttt ttgtaatttt acaggcaacc
    40321 ttttattcac taacaaatag ctggtggaat atatgactat caaagtaggt atcaacggtt
    40381 ttggccgtat cggtcgcatt gttttccgtg ctgctcagaa acgttctgac atcgagatcg
    40441 ttgcaatcaa cgacctgtta gacgctgatt acatggcata catgctgaaa tatgactcca
    40501 ctcacggccg tttcgacggt accgttgaag tgaaagacgg tcatctgatc gttaacggta
    40561 aaaaaatccg tgttaccgct gaacgtgatc cggctaacct gaaatgggac gaagttggtg
    40621 ttgacgttgt cgctgaagca actggtctgt tcctgactga cgaaactgct cgtaaacaca
    40681 tcaccgctgg tgcgaagaaa gtggttatga ctggtccgtc taaagacaac actccgatgt
    40741 tcgttaaagg cgctaacttc gacaaatatg ctggccagga catcgtttcc aacgcttcct
    40801 gcaccaccaa ctgcctggct ccgctggcta aagttatcaa cgataacttc ggcatcatcg
    40861 aaggtctgat gaccaccgtt cacgctacta ccgctactca gaaaaccgtt gatggcccgt
    40921 ctcacaaaga ctggcgcggc ggccgcggcg cttcccagaa catcatcccg tcctctaccg
    40981 gtgctgctaa agctgtaggt aaagtactgc cagaactgaa tggcaaactg actggtatgg
    41041 cgttccgcgt tccgaccccg aacgtatccg tagttgacct gaccgttcgt ctggaaaaag
    41101 ctgcaactta cgagcagatc aaagctgccg ttaaagctgc tgctgaaggc gaaatgaaag
    41161 gcgttctggg ctacaccgaa gatgacgtag tatctaccga tttcaacggc gaagtttgca
    41221 cttccgtgtt cgatgctaaa gctggtatcg ctctgaacga caacttcgtg aaactggtat
    41281 cctggtacga caacgaaacc ggttactcca acaaagttct ggacctgatc gctcacatct
    41341 ccaaataagt tgagatgaca ctgtgatcta aaaagagcga cttcggtcgc tctttttttt
    41401 acctgataaa atgaagttaa aggactgcgt catgattaag aaaatttttg cccttccggt
    41461 catcgaacaa atctcccctg tcctctcccg tcgtaaactg gatgaactgg acctcattgt
    41521 ggtcgatcat ccccaggtaa aagcctcttt tgcattacag ggcgcacacc ttctctcgtg
    41581 gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac aacacaccgt tcaaaaatgg
    41641 cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg tttggtccgg cggcacaaca
    41701 aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg acgctgaaat cacatcgtga
    41761 agatgctgat ggcgtagcgc tgacttttga attgacgcaa agcgaagaga cgaaaaaatt
    41821 ctggccgcac gactttacgc tgttagcgca tttccgcgtg ggtaaaacct gtgaaatcga
    41881 tcttgaatca catggcgaat ttgaaaccac ctctgccctg catacctact ttaacgtggg
    41941 tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc ttcattgata aagtaaatga
    42001 cgcgaaagaa gatgtactga ccgacggtat tcagaccttc cctgaccgta ccgatcgcgt
    42061 gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa gcgctgaatc gtattatcgc
    42121 cgtaggccac cagcatcatc tgaacgttgt cggctggaac ccgggaccgg cgctttcagt
    42181 tagcatgggc gacatgccgg atgatggcta caaaacattt gtttgtgtag aaacggctta
    42241 cgcttcagaa acgcaaaaag tgaccaaaga gaaacctgca catctggcgc aatccattcg
    42301 cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg tgcgatgcat ccggcacgta
    42361 tcaacatcac accatatcca gcgcagtttt tccttttggt gccggatacg ctttatccag
    42421 catgattaat tccgctgacg aaagttcgac ctcaagcaca gccgcattct gttggacatg
    42481 ggcaatcgtg gccgcttttg gaatcgccat cacaccctga tggctgatta cccacgccaa
    42541 caatacttgt gccgcgctga tattgtgagc atgtgcaatt tcgttgacta ccgcgttttt
    42601 taacagtcca ttgcgcaacc gcccggcctg ggctaatgga ctgtaagcca ttaccggcat
    42661 ctgctgttgc tggcaccagg ggagtagatc gtactcaatc cctcgtgaac cgagatggta
    42721 aagcacctga ttagtggcac actgatttcc ccccggcagc tgccagagtt cctgcatatc
    42781 agcataatca aggttagaaa cgccccagcg gcggattttt ccctgggcga tcaatttttc
    42841 catcgctgcc acagtctctt caaaagcgaa actgccagac cagtgtaata agtagagatc
    42901 gagataatca gtattgagac ggcgtaaact ggcttcgcat gcatttatcg ctttttgccc
    42961 gccagcattc cacggataga ctttagagac gagaaagacg ttatcgcgca gatcggttaa
    43021 tgcttcccca accacctttt cggcaccgcc atcggcatac atttcggcgg tatcaatgag
    43081 ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact tctgttttgc gctgactggc
    43141 atcttcgccc atataccatg ttccctgccc tatggctggc agtgagacat cgccactaaa
    43201 ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg caccaccgta atgcaaaaca
    43261 gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa agatgcatta tcagaatttg
    43321 taggtgatcc cggtagaaat caggccagtc caggatttat ccaccatcgg gctgtcagta
    43381 acttcatcag acagacgggt atagcgcgcg gtaccgtaaa cactccagtc gccgaggaag
    43441 ttgtagctgg cgctcagctc caggtaaggg ctccagctgt cgttcgggtt atagccacgc
    43501 agaccgctgc gagcggactc tttgcgcgat acgccataat agtattcgtt ctggttttcg
    43561 ctgttccact gcacaccaat acccggagtc acggtcaggc caccgttggt gtaacgatac
    43621 aaccaggcca tatcccagac aatgccgttg ctgttatcca gggtatcgcc agccagggtg
    43681 gtacgcaggt aaccgtactg ggtaaagtga gcataagaca gaccagccat catggtgctc
    43741 ttacggtcat ccaggtgacg catttggtga tcgccactgt ctttcgcttt gaagtaaagc
    43801 ggcgaccagt aagcggtaat tgaaagttta tccgttgcgt cattccacag gtagtaacca
    43861 ccacctaagc cacggaacca gaagttatcg ccttcatagt tgattaccgg tactgggtaa
    43921 acatcggtat cgtaatcttt atatgggtgc tcaacgacac ctacgcctgc gcccagggaa
    43981 aatttacctt cagcgtgcgc tacgcctgca gacgttgcga taagcactcc aagtgccaga
    44041 agtttgagtt tggtcacaat taatcattcc ttaaacaaat gtttagcggg cgacaaagtt
    44101 tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc attttttaaa gtaactgttt
    44161 aattttcctg acgcggatga caccgcgctt acagccaaat gaatttagcg ttactggcta
    44221 ccctggtctt tacattaatt atgcaaaatt tatggatgag ttgttgatat gccattgaaa
    44281 ttaagaaagc cgtgcaggca agttttccat ttgccatcta cgcttaattt tgaaggtgta
    44341 tcaccgggca cgttgttctc atcgtcgata aaatggcatg agagttgctg tgttttagca
    44401 agagacgtcg ttcagtttac ctcttccggg agcctctact attcatatga acggctctta
    44461 acctgtgcta aaaaacgaaa ggacggcata ccatgaatat attcgatcac tatcgccagc
    44521 gatatgaagc tgccaaggac gaagagttca cactgcagga gtttcttacc acttgtcggc
    44581 aagatcgcag tgcttatgcc aacgcggctg agcggctatt gatggctatc ggtgagcctg
    44641 tcatggtcga tacagcccag gaacccagac tttctcgact cttttctaac cgggtcattg
    44701 cacgttatcc ggcgtttgaa gagttttacg gcatggaaga cgcgattgaa cagattgtct
    44761 cttatctgaa acacgcggct caggggctgg aagagaagaa acaaatcctg tatctgctgg
    44821 ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact gaaatcatta atgcagctcg
    44881 taccgattta tgtattgagc gccaacggtg agcgtagccc ggtcaacgat catccgttct
    44941 gtcttttcaa tccgcaggaa gatgctcaga ttctggaaaa agagtatggc attcctcgcc
    45001 gttatctcgg caccatcatg tcgccatggg cggcaaaacg cctgcatgaa tttggtggcg
    45061 atatcactaa gttccgggta gtgaaggtct ggccgtcaat tctgcaacaa attgctatcg
    45121 ccaaaacgga acccggtgat gagaacaacc aggacatctc cgcgctggtt gggaaagtcg
    45181 atattcgtaa actcgaacac tacgcgcaga atgacccgga cgcctacggc tattccggtg
    45241 cgctgtgccg cgccaaccaa gggatcatgg agttcgttga gatgtttaaa gcaccgataa
    45301 aagtgctgca tccgctgtta accgccactc aggaaggtaa ctacaacggg acggaaggta
    45361 tctccgccct gccgttcaac gggattatcc tcgcccactc gaacgaatcc gaatgggtca
    45421 ctttccgtaa taacaaaaac aacgaagcct ttctcgaccg tgtttacatc gtgaaggtgc
    45481 cgtattgctt gcgcatttcc gaagagatca aaatctacga gaaattgctt aatcacagtg
    45541 aattgactca cgccccatgc gcccctggca cgctcgaaac actgtcacgt ttttccattc
    45601 tttcgcgcct gaaagagcca gaaaactcca gcatttattc aaagatgcgg gtttatgatg
    45661 gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta tcaggaatat cgtgactacg
    45721 ccggtgtcga tgaagggatg aacggtctgt cgacgcgttt tgcgtttaag atcctctccc
    45781 gcgtgttcaa cttcgatcat gtagaagtgg cagcaaaccc ggtccatctg ttctacgtcc
    45841 tggaacagca gatcgagcgc gagcagttcc cacaagagca ggcagaacgc tatctggagt
    45901 tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat cggcaaagag atccagacgg
    45961 cctaccttga atcctattcc gaatatgggc aaaacatttt cgaccgttat gttacctacg
    46021 cggatttctg gattcaggat caggagtatc gcgatccgga taccgggcag ctgtttgacc
    46081 gcgagtctct taacgccgag ctggagaaaa tcgagaaacc ggcggggatc agtaatccaa
    46141 aagatttccg caacgagatt gttaacttcg tactgcgcgc cagagcgaat aacagcggac
    46201 gaaatccgaa ctggagcagc tatgaaaaac tgcgcacggt tatcgagaag aaaatgttct
    46261 ccaataccga ggagctgttg ccggttatct cgtttaacgc caaaacgtca accgacgagc
    46321 agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa aggctacacc cgtaaacagg
    46381 tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc gtcttaacaa ccctggcccg
    46441 gtacacatgc taccgggcct acaacgacag cgaaccgtgg gccagagaaa tggcaacaca
    46501 ggcgtagcat acagttggca aatgtagtac ggggggcata tgacctggtt tattgaccgg
    46561 cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc gttttttacg ccgttataaa
    46621 gcgcaaatta aacagtcgat ctccgaggcc attaataagc gttcggtgac tgacgtcgac
    46681 agcggcgaat ccgtatccat tcccacggaa gatattagcg aaccgatgtt tcatcagggg
    46741 cgtggcggtc tgcgccaccg cgtgcatccg ggcaatgacc atttcgtcca gaacgaccga
    46801 attgaacgtc cccagggtgg cggcggaggt tccggcagtg gacagggcca ggccagccag
    46861 gatggtgaag gtcaggatga atttgtcttt cagatttcga aagatgagta tcttgatctg
    46921 ctctttgaag atttggcttt accgaatctg aaacaaaacc aacaacgcca gctgaccgaa
    46981 tataaaacgc atcgggcggg ttataccgct aacggcgttc cggccaatat cagcgttgta
    47041 cgttccttgc agaactcact ggcgcgtcgc acagctatga cggcgggcaa gcgacgggaa
    47101 cttcatgcgc tggaagagaa tttggccatc atcagcaaca gtgaacctgc gcaactgctg
    47161 gaagaggaac gtctgcgcaa agaaattgca gaattacgtg ccaaaattga acgcgtccct
    47221 ttcattgaca ccttcgattt acgttacaag aactacgaga agcggcccga tccctccagc
    47281 caggcagtga tgttttgcct gatggacgtt tccggttcaa tggatcaatc cactaaagat
    47341 atggctaagc gtttttatat tctgctgtat ctgttcctca gcagaacgta taagaacgtg
    47401 gaagtcgtat acatccgcca tcatacccag gcgaaagaag tcgatgaaca tgagtttttc
    47461 tactcgcagg aaacaggcgg caccattgtt tccagcgccc tgaaactgat ggatgaggta
    47521 gtgaaagagc gttataaccc ggcacagtgg aatatttacg ctgcacaagc atcggacggc
    47581 gataactggg ccgatgactc tccgctttgc catgaaatcc tggcgaaaaa attattacct
    47641 gttgttcgtt attacagcta tatcgaaatt acccgtcgtg cacatcagac attgtggcga
    47701 gaatatgagc atctgcaatc tactttcgac aactttgcga tgcagcacat ccgcgaccag
    47761 gatgatattt atccggtatt ccgtgaactg tttcataaac aaaatgcaac agctaaagac
    47821 taaaactatc agccgggtca ttatcgcctg gctgattttt agcttactgt aaattatctc
    47881 atttattaca tacattagct tacaatcgct ttaaatatga cagcataacc tttacataat
    47941 ttagttccag aaaacaatca ttcggaaaaa tgattcagtc aacacgtatt tccatggggt
    48001 tattctttaa atatttttta tcgttaacga aaattgatcc tggtcaaaac tatatatctc
    48061 tgccatcaat aaaatccagc actcacattg ctctcctttt tatggtttct atgggtacac
    48121 aaaaattaaa agctcaaagc ttttttattt tcagtttatt gctgacgtta attttatttt
    48181 gcatcaccac attatataac gaaaacacga atgtaaaact catcccacag atgaattatc
    48241 tgatggtcgt tgtggctttg tttttcctta acgccatcat ttttcttttc atgttaatga
    48301 aatatttcac taacaaacaa attctaccaa cactcatttt aagtcttgca tttttaagtg
    48361 gccttatcta tttagttgaa actattgttg ttatccataa acctatcagc gacagtacac
    48421 tgatccagac aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttta
    48481 tttgtttaac ctcgctggcg ctcttttgtt atggtaaaga caacatcctt gacaacaata
    48541 agaaaaaaac tggcattctg ttgctggcgc tgattccttt tttagttttt ccccttctgg
    48601 cacacaatct gagcagttat aacgctgact attctttgta tgtcgtcgat tactgtccgg
    48661 acaaccatac tgcgacctgg ggaatcaact atacaaaaat attggtttgt ctgtgggcat
    48721 ttttactgtt ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag
    48781 cattattatg tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata
    48841 attacaccat ctggtatatc agtcgcggga ttgaagtttc cagtaaactg tttgttgtgt
    48901 cttttctgat ttataacatt tttcaggaac tgcaactctc cagcaaactg gcagttcatg
    48961 atgtgctgac caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt
    49021 cgcgacctgt tgttaaggac ttttgtgtca tgctggttga tattaatcag ttcaaacgca
    49081 tcaatgccca atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta
    49141 tccagcaaag catccgcccc gatgatattt tagcgcgact ggagggtgag gtgtttggct
    49201 tgctatttac cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa
    49261 atgtcgaact cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca
    49321 gtattggcac ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc atgacggaag
    49381 cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata
    49441 tttaagtgca aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta
    49501 ctattacccc taaatttccc ctccccagcg gcgcagagat gagtataatt agcgcccctg
    49561 tgccaggccg caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat
    49621 ttatcatgaa attgcaccat agaatgctcc ggcattttat cgccgcaagt gtcattgtgc
    49681 tgacatcttc cttccttatt tttgaacttg tcgccagcga cagagcaatg agcgcctatc
    49741 tgcgctatat tgtgcagaaa gcagactcct cctttcttta tgataagtat cagaatcaga
    49801 gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg tcgccagaaa
    49861 agcggcgcgc catctgcgag gcttttgagt ctgccaataa tacccatggc ttaaacctga
    49921 ctgcccataa atacccgggc ttacgcggca cactacaaac cgcatccact gactgcgaca
    49981 caattgtgga agctgcagcg ctattacccg cttttgatca ggcagtggaa ggcaaccgcc
    50041 accaggatga ttacggttca ggtcttggga tggccgaaga aaaatttcac tattatctcg
    50101 atctcaatga ccgctatgtc tatttttatg agccggttaa tgttgaatac tttgcgatga
    50161 ataactggtc cttcctgcag tcaggaagta ttggcatcga tcgcaaagat attgaaaagg
    50221 tatttaccgg gcgtaccgta ttgtcgagca tttaccagga tcagcgtact aaacagaacg
    50281 tgatgagttt gctgacgccg gtatatgtcg cagggcagct aaaagggatt gtgctgctgg
    50341 atattaacaa aaacaatctg cggaatatct tttatactca tgaccgccct ctcctctggc
    50401 gttttctcaa tgtcacgcta accgataccg attcggggcg cgacattatc atcaaccaga
    50461 gcgaagataa tctgttccag tatgtcagtt acgtccatga cttaccgggc ggcattcgtg
    50521 tctcgttatc cattgatatt ctttacttta tcacgtcttc gtggaaaagc gttctgttct
    50581 ggattttgac ggcgttaatt ttgctgaata tggtccggat gcacttccgt ttataccaaa
    50641 atgtgtcgcg agaaaatatt agtgatgcaa tgactggact gtataatcgc aaaattttaa
    50701 cccctgaact ggaacagcgg ttgcagaaac tggtgcaatc cggttcttcg gtgatgttta
    50761 ttgctattga catggacaag ttaaagcaaa taaatgacac cctcggtcat caggaggggg
    50821 atttagcgat tacgttatta gctcaggcga ttaaacagtc gattcgtaaa agtgattatg
    50881 ccatccgact cggtggcgat gaattctgca tcattcttgt cgattcgacg ccgcaaattg
    50941 cagcacaact gcctgaacgt atcgaaaaac gtctgcaaca tatcgcgccg cagaaagaga
    51001 tcggcttctc ttccggtatt tatgcgatga aagaaaacga tacgttacat gatgcgtata
    51061 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
    51121 cttctgtggt tgtttgcttg taatctcagg agcatgaaat gactgaaatg gctaaaggaa
    51181 gcgtgacgca tcaacggtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
    51241 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctttag
    51301 gccagggagc taaggcactg gtatgtaagg tcaaaggcaa tggcgtaaat caacatgttc
    51361 tggcgatcct cgctgccgat caacaggccg atctgagcca actcgccagc catataggcg
    51421 gattacgcgc ttcgttggcc agcccggcgg aagtcgatga actcacaggc tgtgtcttcg
    51481 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
    51541 tcgaacgatt tgatgaaatc gccttcaatg caggcatgct ggataaatcc gttattttga
    51601 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
    51661 aactagcctg tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatggcaaaa
    51721 aacgatgacg agataatcag tgtctcgacg aacatttgat cgttcatagt atgcccccgg
    51781 gagatagctg ttttcacgtt attgttagcg tgcaccaatg gcagtttgat gacagttcgc
    51841 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag
    51901 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
    51961 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
    52021 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
    52081 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
    52141 gggacgctac cgccctcaac cctgattcac tcgtttctga actggaaatc gctggtggca
    52201 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
    52261 cagccgcaac tggttgctgg gctgctggtg ggcactgttt tgggcgtggc gctgtttcgc
    52321 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
    52381 tagttaatcc cgcaatatat cggcctggcg tttgccccag gcctttctta aacatagtaa
    52441 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
    52501 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg tcgccactgg cgaaaactca
    52561 accccgtctc ttttactatc agtcgggcaa tattgcgttc actcatcgca aaaaagcaag
    52621 cccattgccc caatgacccc cactcgacgg gctctttcgc catcatctcc accatcgcgc
    52681 ggattttagg atgagaagaa acgggtaggt gcagctgttg ttgtggctgc tgagggagtt
    52741 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
    52801 tgtttgccag agttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacagg
    52861 ttgtcggcat tatcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg
    52921 tcacttgatt gctatgttcc acccctcccg gtatccaaac cgcatattgc ggcggcacca
    52981 tccacaaagc attttccacc gtacaggtaa ttgcaccatg tagcgccagt atcagctgtc
    53041 ctttgcggtg ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg
    53101 cgaccgcggc ttcatggtgt cggtcgggtt catagccatt gagattcaac ctgtgcatca
    53161 ttttgtccga atttagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac
    53221 ggtaaggtac agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag
    53281 cggcaaaaac aggattgtcc ttatcgctgg cattctgatg attgccacaa cattacgcgt
    53341 cacctttacc ggcgcagcgc cgttactgga tacgattcgt tccgcttact cgctgacgac
    53401 agcgcaaacc ggcttattaa ccaccctgcc attattggcc tttgcgctaa tctcaccttt
    53461 ggctgccccg gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt
    53521 gatctgtgca ggtatcgcaa ttcgttctct cccttcgcct tacttattat ttggcggtac
    53581 agcggtcatt ggcggtggga ttgcattagg caatgtctta ctgccaggat taattaaacg
    53641 cgatttccct cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc
    53701 agcggcactg ggatcggcta tggtcgtgcc gctggctttg aacggttttg gctggcaagg
    53761 cgcgttgttc atgctgatgt gttttcctct gctggctctt tttttatggc tgccacagtg
    53821 gcgaagccaa caacatgcaa atttgagtac ctcgcgcgcc ttacatactc ggggtatctg
    53881 gcgttcgccg cttgcctggc aggtcacatt gtttcttggg atcaactcac tggtctatta
    53941 cgtgattatt ggctggcttc cggcgatcct catcagtcgc ggctatagcg aagcacaggc
    54001 gggttcactg catggtttgc tgcaactagc cacagcagct cccggtttgc tgatccctct
    54061 tttcttacat catgtgaaag atcagcgtgg tattgcagcg ttcgttgcct tgatgtgcgc
    54121 agtgggcgct gttgggctct gctttatgcc agcgcacgcg atcacctgga ctctgctttt
    54181 cggttttggt tccggcgcaa caatgatact ggggttgacg ttcattggtc ttcgggcaag
    54241 ttctgcgcat caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc
    54301 agcctgtggg ccgccgctga tgggtaaaat acacgatgct aacggtgact ggtccgtacc
    54361 actgatgggt gttgccatac tttcactact gatggcgatt ttcggacttt gcgccgggag
    54421 agacaaagaa attcgctaat atccggtgct atagtgacgt gacaaatcat gcgtgaaagg
    54481 gagaacaaac acgatgaata ttcagtgcaa acgcgtttat gatccggctg aacagagcga
    54541 tggttatcgc gtactggtcg accgcctctg gccgcgcggt atcaaaaaaa ccgatttagc
    54601 ccttgatgag tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg
    54661 cgaagtcgtc gattttgcaa cctttcgcga gcaatatctt gcagaactgg cgcaacacga
    54721 gcaagaagga aagcggctgg cggacatcgc caaaaaacag cccctgaccc tgctctacgc
    54781 agcaaaaaac accacgcaga accatgcgct ggtgctggcc gactggctac gtagcttgtg
    54841 attttagtac agcatccggc ggttattttt caccagccgg atggtaccgt cgccacaatg
    54901 cccattcatc aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgctg
    54961 tctgcactgg aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca
    55021 taccaatctg cggcggttta ggtgcttctg gctgagaagg ggttgaacaa ccagccagga
    55081 ccagcaagca aggcagaaca aaactgataa ttttcattta ttgatctcac atatttatcc
    55141 agacttagag tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca
    55201 aatcatatta cgccttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta
    55261 aataatcagc atttacccgc cgtatcctgg agttgtttcg tgtcagatca gattatcgcc
    55321 cgcgtctcgc aatcccttgc caaagaacag tcactggaaa gtctggtccg acagcttctg
    55381 gagatgctgg aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa
    55441 gcgcgcctgc agcatattat gtttgcccgt aacagccaga aaatgcacat cccggagaat
    55501 tttaccgtct cgtgggatta ctcgttatgc aaacgcgcca ttgatgaaaa ttgctttttc
    55561 agcgatgaag tccctgaccg ttggggcgac tgtattgcgg cacgcaatct tggcatcacc
    55621 acatttctga gcacgccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc
    55681 gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac aggttttaca gttattcgct
    55741 ggactgattg cacaatatat tcaaaaagag gcgctggttg aacagctgcg cgaagccaat
    55801 gctgcgctga ttgcgcaatc gtataccgac tcgttaaccg ggctaccgaa tcggcgggcg
    55861 atttttgaaa atctgacgac gctgttttct ctcgcccggc atcttaacca taagataatg
    55921 atcgcgttta tcgatctgga taacttcaaa ttaatcaatg accgttttgg tcataatagt
    55981 ggcgatctgt ttctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa
    56041 gttattggtc gtctcggcgg tgatgagttt ttggttgttt cactaaacaa cgagaatgcg
    56101 gatatttcgt cgctgcgaga acgtattcaa cagcaaatac gtggagaata tcacttagat
    56161 gatgttgatt tgtattatcc cggtgccagt cttggcatag tagaagtcga tcctgaaaca
    56221 accgatgcag acagtgccct gcatgctgcc gatattgcga tgtatcagga gaaaaaacac
    56281 aaacagaaaa caccttttgt cgcgcatcca gcgctacatt cctgaggcgt attcacatcc
    56341 ttttgattgg tgataacatg cgaatcggta ttatttttcc ggttgtaatc tttattacag
    56401 cggtcgtatt tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat
    56461 gaaaaaaaca acgattatta tgatgggtgt ggcgattatt gtcgttctcg gcactgagct
    56521 gggatggtgg taacgtcacc tctaaaaaat agcaaaggct gcctgtgtgc agcctttgtg
    56581 caatttaagc gttaactttt aatcttcctg tagataaata gcacgacaat cgcaccaata
    56641 acggcaacca cgaagctgcc aaaattgaag ccatcgactt taccaaagcc aaacagtgtg
    56701 ctgatccatc cgccgactac ggcaccgact atccccagca gaatagtcat aaagaatcca
    56761 ccaccatctt tacctggcat gatccacttc gccagaatac cggcaataag cccaaaaata
    56821 atccatgaca gaatgcccat tgtttcctca cttatctgtt ttgcattagc gggttagtcg
    56881 ctgataaaaa gcatagcaca acatcgggag ggcaagattt gtgacgagca tcacggagga
    56941 tttttttgcg gatggcacag aaattgcgcc atcaacgatc agtgataatt accaaccaca
    57001 aacatcatgt tcgttttccg tgtcttgaca tcctccacgc cctgaatgac gaggatccct
    57061 gctacgttca ggctgtcgcc tgaatcattt cggtgggttc ctgcttcaac gagcggcctg
    57121 actgcaccat ctctccacag gcaagaacgg cgtgccccgc cgctaaaatg ttacgagcgc
    57181 cgtttacatc ggcgttcgct gtatatccac atacctggca tctgaattga ctttgtgaca
    57241 ggcgattttc tttcgctgta tgaccacagc acgcgcaacg ctggcttgtg tacgctggcg
    57301 gaacagcaag cacctgaccg ccacgccaga gctgcttgta ctcaagctgg cggcgcattt
    57361 cataccagcc ctgatccagt atcgaacggt ttaaacctga ttttgcccgg acattgcgcc
    57421 ccggctggct gaccgtaccc gctgctgact ttgacatgtt gctgaccttc aaatcctcaa
    57481 tgactatcat tgcgtggttt ttgctgacgg tcgttgtgac tttgtgaagg tagtccctgc
    57541 ggatatttgc gatacgggaa tgcagtcgct gtattttgcg tttctgcttc tgccagttgt
    57601 tgctgaattt gaccttgcgg cttaactgtc gctgaagtct cgccagcgtc ttctggtttt
    57661 tctgaaaact gtttacaggc tcaaagactg tgccatctga cagcgtggcg agtttagcca
    57721 cgccagcatc cagcccgacc attgatgctg aagggtgaac cggagttgat acttcacttt
    57781 ctgtctgaat actgatgtac cacttaccgc aggactggct gacagtgaca tttttcacaa
    57841 cacccgtgac ctgacggcta ttccggtagc gcatccagcc cagtttcggc agaaaaatac
    57901 ggctgttttc ctgatcgagc ttaacgccct gcgggtagcg gaatgcatca ttctgtcccc
    57961 gctttttgaa tcggggaaaa gcagcccgct tccggaagaa gtttttgtaa gcccgctcaa
    58021 ggtctttcag tgactgttgc aatggctgtg agggagaatc tttaagccat tgcgtttcag
    58081 tggcattttt ccactcaacc agccaggaag ccattttccc gtaagggatg tatttattac
    58141 cggcctcatg attctcattc tgaagcgcca gagcacgatt gaaaacgaaa cgacatgcgc
    58201 ctgcgaagcg cctcatttca cactcctgtt gatcacctgg tcttaactgg aatttaaatg
    58261 cctgtagtcg tttcacaggc caaatgatac tttggtctgt gaaaaaagaa accgatatcc
    58321 ggcgtggaag gcattgtgtt ttcctgatgc atgttcacct ggtctttgtc accagataca
    58381 gacgtcagat ttttgaccat gacgcgacag aaaaactacg cgcttacttt tcaaatgtat
    58441 gtgctgattt tgaagctgaa cttgttgaaa tggatggcga accagatcac gtccatttgt
    58501 taattaacta tcctcccaaa ctggcgatat ccagtctggt aaacagcctc aaaggcgtat
    58561 cgggtaggtt actgcgacga gatcgaccag atattgcagt caggtattac tacaaaggcg
    58621 ttttgtggag tcctggctat tttgccagta gctgcggagg tgcgccaata tccgtcatcc
    58681 gccaatacat tgaacaacag caaacacctg gttaggtgga aaaccgcgcc ttatatcccc
    58741 gccctgaagg acggggtttt acggcgcacc ggataagaac gcacggtatt caccagatct
    58801 tttatcactt cagccgccac ttctggcacc agcaaagtca tcggcgtctc tgtttcataa
    58861 tcgacagaaa cgccattgct gttattggtg acggtcacgg tatacgttgc tttgcccatg
    58921 attcatttcc cgttatgaat gactttccgt tgttgcgcac cttccatcag gacttcagga
    58981 gccacgaaga agtcaatgtt gaaataagta tcgtcagtca tggcttcaat gttgtgccac
    59041 ttttctggag ggaacaccgc aaactgcccc gcttcgataa ggatcacctg atcaggctct
    59101 gcactgtgtt catcagcgta gccgagatat ttgaccgccc catgcataac ggaaaggcgt
    59161 gggtaaaccc ccgggcgcgt tcctttatca agatgacgtt cgaatattcc ggcaggcgca
    59221 gtttgtttat tccagaaagg cgttgagcgc gtatgaatat aattctgtgg gatttgcagc
    59281 atctttttcc ctccttcgtt gaatgcgctg aaaacggtat tccagccgtt tcagagtacg
    59341 cctgataatt tgcattttaa ataccattta ttggttattt tttagcatca tatcagagaa
    59401 gaatcaggga ggattacaag tgggaagccc gcgacaacgc gggctgaatg tatcaggatt
    59461 gcagcgtcgc cagtcgggca gcgaaaccca cgaacatcaa accaatcagt gagttgccca
    59521 ctttagtcag tttctttttg gtacgtatgt actgcgtgac aaaagcacca gaaataatca
    59581 ggaagctcaa atagcagaaa ctcaccagtt ccagcgtcgt cgccagaata aagaatgaaa
    59641 ttcccgtatg tggggcatta acatcgataa actgtacgaa aaacgacaca tagaacaaaa
    59701 tggctttcgg attagtcagg ctcaaaatta acgcgcgttt aaaaatagca ccgtattggg
    59761 gctcatcgga tttggcctcg ttatttttac ccttcagggt cgcgtagaga attttactcc
    59821 ccagatagag caaataaaac gcaccaagat aacgtataat gttgaataat atcggggtgg
    59881 tcttaattaa tgtcgccact ccagcccatg ccagaaacat caataccgca tcgccaataa
    59941 atacaccgca ggccgcaaga taaccgcctt tcataccgct actgacgcta tttttgagta
    60001 caaacagggt atttggccct ggcaccaaca caataaaaat ggccccaacc agataggtcc
    60061 agtaattcag aaccccgtat tcagcgaaca cattaacctc tttaattatc tttcgatcat
    60121 gcgcgattaa aggtgaatat gctaaccaat ctgtagcggc ttagaaagga gaaaatcagg
    60181 ttttaacccg atatcaaccc gataattgaa taattaatca ggcatgctcc agtgaaaaat
    60241 tctggtagtg ctcggcaaaa tactggcgta aaaattctac cgttatccgc actttcgctg
    60301 acgtcgccag ccttgaaaca taaacggccc agacgttcgc tggctggtaa tattccggta
    60361 gcacttgcac taaatgacca ctggcaatgt tttcgctaac atcccaccag gaacgcagag
    60421 caatcccctg cccatcgaga caccactgat ggacaatttc tccatgatta gacgacagtg
    60481 ggccggtgac tttaatcgca tggtggcctt ctttattgcg taattgccag acaccaaaag
    60541 gatgatcgcg ctctttaatg accaagcacg gcaatgcaga aagatcggtt aaatgtttcg
    60601 gcgcgccatg ttgcgcaata aattccggcg acgcgcagag aatgcggtaa ttggtcgcca
    60661 gtttgcgggc gattaaatta ggggcgatgt catcgccaat gcgaatatcg agatcgacac
    60721 cttcattgac caaatcgacc agtcggtctt ccacatcaaa acgtaattcc agttgcggat
    60781 acgccttcgc cagcgctgat aacgccggag ccaccacctg tcgtccaaaa ccaaagctgc
    60841 tgataatacg cagcatcccc tggggcacct gacgcacgtc agaaagttcg tccatcatct
    60901 gaccgacatc ctgcaaaatc cgctgcgccc attcataaat ccgctctcct tcttcggtaa
    60961 tggtgacgcg gcgggtggtg cggtgtaaca acaccacgtt tagcgtttgc tccagcaagg
    61021 cgatgcgctt gctgacgaac gccggtgaaa cgcccagttc ttccgccacg gcggcaaaac
    61081 cagcccgacg agccaccagc ataaagacgc gcaaatcatt cagcagcggt aaattattca
    61141 tgattcgtgt tttatgtttc accagttacg gggattaatt ccttttcagt caattatagg
    61201 atggtgatgt tgtcaatttt gatggtcagg aagtgagaac ccaatgatga aaacgatgcg
    61261 tattgctgcg atcccgggag acgggattgg caaagaagtc cttcctgaag ggattcgcgt
    61321 gttacaggct gccgctgagc gctggggctt cgacttgagt tttgagcaaa tggagtgggc
    61381 gagctgcgag tattacagcc atcacggtaa aatgatgccg gacgactggc atgagcaact
    61441 tagccgtttc gacgccatct attttggtgc cgtcggctgg ccggataccg ttccggacca
    61501 tatttcgttg tggggttcgc tgctgaaatt tcgtcgtgaa ttcgaccagt acgtcaacct
    61561 gcgcccggtt cgtctctttc ctggcgttcc ctgcccgctg gcgggaaaac agcctggcga
    61621 catcgatttt tacgtggtca gggaaaacac cgaaggcgaa tattcctcgc tcggcggtag
    61681 agtgaatgaa ggtacagagc atgaagtcgt cattcaggaa tcggtattta cgcgtcgtgg
    61741 tgtcgatcgc attttgcgtt atgccttcga acttgcgcaa agccgcccac gtaagacgct
    61801 aacttctgcc actaaatcaa acggtttagc catcagcatg ccgtactggg atgagcgagt
    61861 ggaagcaatg gccgagaatt acccggagat ccgctgggac aagcagcata ttgatattct
    61921 ctgcgcgcgt tttgtgatgc agccggaacg cttcgatgtg gtggtggcgt ccaatttatt
    61981 tggcgatatc ctttccgatc ttggcccggc ctgcaccggc accattggca ttgccccatc
    62041 cgccaacctg aatccggaac gcactttccc gtcgctcttc gagccagtcc acggttccgc
    62101 gccggatatc tacgggaaaa acattgctaa ccctatcgcc acgatttggg ccggtgcaat
    62161 gatgctcgat tttctcggca atggcgatga gcgtttccag caagcgcata acggtattct
    62221 ggcagcaatt gaagaagtga ttgctcacgg gccgaaaaca cctgatatga aaggcaatgc
    62281 caccacgcca caggttgccg acgcgatttg caaaattatt ttgcgttaag gtcaaaccag
    62341 tttatttgaa ccgcgtcact gacgcggttt ttttattcgt tctttgcagt aaataacctg
    62401 cgtcatttca ccttttattg tttccgtttc gtgttttatg gctttccgta ttcttaattg
    62461 tttaatttat gtaacatgca aataatcaat ttgttaaaca caacatacaa ctcattaccc
    62521 tgcaaataat tacaactggc aaaagccaga gacgggcttt tatgcccagg agatgactca
    62581 tgatgagcaa tgttaagaaa aaagatgtgc cgctgataag catcagcctg gtggccattc
    62641 ttttcatcgc agctgcatta agccttttcc cacaacaatc ggccgacgcg gccaacgcaa
    62701 tatacacttt tgttacgcgt acgttaggtt ccgccgtaca ggtattggtt ttgctggcaa
    62761 tgggactggt gatttattta gccaccagta aatacggcaa tattcgtctt ggcgaaggaa
    62821 aaccggaata cagcacgctc tcctggctgt ttatgtttat ttgtgccggt ttaggttctt
    62881 ctacgcttta ttggggggtt gctgaatggg cctattatta tcaaacgcct ggattaaata
    62941 tcgcaccgcg ttcacaacag gcactcgaat ttagcgtccc ttactccttc ttccactggg
    63001 gcatcagcgc ctgggcaact tatacgctgg cctcattaat catggcttat cactttcatg
    63061 tgcgtaaaaa caaaggtctg agtctttccg gcattattgc cgcgattacc ggcgttcgcc
    63121 cgcaaggccc atggggaaaa ctggttgatt tgatgttcct gatcgccact gtcggcgcac
    63181 tgactatttc ccttgttgtc accgcagcaa cctttactcg tggactttcc gcgctgaccg
    63241 gtttacccga taacttcacc gtgcaggcat ttgtgatcct gctttccggc ggcatttttt
    63301 gcctaagctc gtggattggt atcaacaacg gtttgcaacg tctgagcaaa atggttggct
    63361 ggggcgcgtt cctgctgcca ttactggtgc tgattgtcgg cccaaccgaa tttattacca
    63421 acagcatcat caatgccatc ggtctgacca cgcaaaactt cctgcaaatg agcttattca
    63481 ccgatccgct tggcgatggt tcatttaccc gcaactggac cgttttctac tggctgtggt
    63541 ggatctcata cacccctggc gtagcaatgt ttctcacccg cgtttcccgc ggtcgtaaga
    63601 ttaaagaagt tatctgggga ctgatcctcg gcagcaccgt cggttgctgg ttcttcttcg
    63661 gcgtaatgga aagctatgcc attcatcagt ttatcaatgg cgtaatcaac gtcccacagg
    63721 tgctggaaac actgggcggc gaaacagctg tacagcaagt tctgatgtcg ttgccagccg
    63781 gtaaattgtt cctcgccgca tacctgggcg tgatgattat tttccttgcc tcgcatatgg
    63841 atgcggtggc ctacaccatg gctgcgacca gtacgcgtaa tctccaggaa ggtgacgatc
    63901 ctgaccgtgg gctgcgtctt ttctggtgcg tggtgatcac tctgatcccg ctttccatct
    63961 tgtttaccgg tgcttcgctg gaaacgatga aaaccaccgt cgtgctcaca gcccttccct
    64021 tcctcgtcat tttactggtg aaagtcggcg gatttattcg ctggctgaaa caggattacg
    64081 ccgacattcc ggctcatcaa gttgaacatt atctcccgca gacaccggtt gaagccctgg
    64141 aaaaaacgcc agtgctccct gcgggaaccg tattcaaagg cgacaactga gcgccatcat
    64201 cctaacgata aaggtatccc tatgagcaat ctgagccctg actttgtact acccgaaaat
    64261 tattgcgcta acccgcaaga ggcgtggacc attcctgccc gtttttatac cgatcagaac
    64321 gcgtttgaac acgaaaaaga gaacgtcttc gccaaaagct ggatttgcgt cgctcacagc
    64381 agcgaactgg cgaatgccaa tgattatgtg acgcgtgaga tcattggcga aagcatcgtg
    64441 ctggtacgcg gtcgtgataa ggttttgcgc gcgttctata acgtgtgtcc gcaccgtggt
    64501 catcagttgt tgagcggtga aggaaaagca aaaaatgtga ttacctgccc gtatcacgca
    64561 tgggcattca aactcgatgg caacctggcc catgcacgta actgcgaaaa cgtcgccaat
    64621 ttcgatagcg acaaagcgca actggttccg gtgcgtctgg aagaatatgc cggattcgtc
    64681 ttcatcaaca tggaccccaa cgccaccagc gtagaagatc aattacccgg cctgggcgcg
    64741 aaagtgctgg aagcctgccc ggaagtccac gatctgaaac tggcggcccg ctttaccacc
    64801 cgcacgcctg ccaactggaa gaacattgtc gataactatc tcgagtgcta tcactgtggt
    64861 ccggcgcatc caggtttctc cgactccgta caggttgatc gttactggca caccatgcac
    64921 ggtaactgga cgctgcaata cggtttcgcc aaaccgtccg aacagtcgtt taaatttgaa
    64981 gagggtacgg atgcggcatt ccacggtttc tggctgtggc cgtgcacgat gctgaacgtc
    65041 accccgatca aagggatgat gacggtcatt tatgaattcc cggtggattc tgaaactacc
    65101 ctgcaaaact acgatattta cttcaccaat gaagagttaa ccgacgagca aaaatcgctg
    65161 attgagtggt atcgcgatgt gttccgtccg gaagatttac gtctggttga aagcgtacag
    65221 aaagggctga aatcgcgtgg ctatcgtggt caggggcgca tcatggccga cagtagcggt
    65281 agtggcattt ccgaacatgg tatcgcccat ttccataatc tgctggcgca ggtgtttaag
    65341 gactaatgac atcggcggcg gtattttccg ccgctgggct gatttttgat ggagtacagc
    65401 aatgtcagac tatcaaatgt ttgaagtaca ggtgagtcag gtggaaccgc ttaccgaaca
    65461 ggtgaaacgc ttcacgctgg tggcaaccga tggcaaaccg ctgcctgcgt tcaccggagg
    65521 aagtcacatc attgtgcaga tgagcgatgg tgataaccag tacagcaatg cgtattcact
    65581 actgagttcg ccgcatgaca cctcttgtta tcagattgcc gttcggctgg aggaaaactc
    65641 gcgcggcggt tcccgctttt tgcatcagca ggtaaaagtg ggcgatcggt taacgatttc
    65701 aacgcctaat aacctgtttg cgctaattcc ctcagccaga aagcatctgt ttatcgcggg
    65761 cggtattggt atcacccctt tcctgtcgca catggcagag ctgcaacaca gcgacgtcga
    65821 ctggcagcta cattactgct cgcgaaatcc agaaagttgc gcatttcgtg atgagctagt
    65881 ccagcatccg caggctgaga aagttcactt gcatcactca tcaaccggaa cacgactgga
    65941 attagcgcga ttattggcgg atatcgaacc tggcacacac gtttatacct gtggtcccga
    66001 ggcgctaaat gaagcggtaa gaagtgaagc tgcgcgtctg gacatcgtcg ccgatacgct
    66061 gcactttgag caatttgcta tcgaagacaa aaccggcgat gcctttaccc tggtgcttgc
    66121 ccgttccgga aaagagtttg tggtgccgga agagatgact attttgcagg ttattgaaaa
    66181 taataaagcc gcgaaagtgg aatgtttatg tcgtgaaggg gtatgcggaa cctgcgaaac
    66241 agcaatactg gaaggtgaag ctgaccatcg ggatcagtat tttagcgatg aagagcgtgc
    66301 cagccagcaa agtatgttga tctgttgttc gcgtgcgaag ggtaaacgcc tggtgttgga
    66361 tttgtagttt gccaaagccg gatgtggcgc tgagcgcgcc cagtccggct tcggaagatt
    66421 ttactgcgga tattcctgca ataaattgtg taatgcttcc gccatcagct caccacgcca
    66481 gccggaaatc agctccggca aattgttctg cggtttcagt ttccagtgcc agttcagcag
    66541 ttggttgatt tgccgacgcg atgccagcaa ttcggcgctg atcttatgcg tttcgctcac
    66601 gtcagtaatc agcgacttaa tcgctttaaa cgctttacga taaccaggca tgtccatcag
    66661 gttaagcatc ggctgcggta aggcctcttc cggcaatgtc tgcgcttttt ccaccagcgc
    66721 cagcagcgtt ttaccgtgaa agcggatttc gctaccggat aaacccaggc tgtccagttc
    66781 gcctaaactt cccggcatat aacgcgcgac cgaccacaaa tgctcttcac gcacgacaaa
    66841 gttcaccgcc agatcacgct ctcgcgcctt gcgcagtcgc cagtcggcta acagttgcag
    66901 acaggccagt tggcgtgtgc gtaactgcca ggcattggtg atatcacgcc aggcatcttc
    66961 cggcgcaacg acttcctgac gacgcatttg catcaggcgg cattcatcca gcgccgcagg
    67021 tagccagccg gaggcctccg tttctaccat aagcttggcg gtgatcggta acagatacca
    67081 gacatccgcc gctgcgtatt cacactgacg ttcggtcagc ggtctggcca gccagtcggt
    67141 gcgcgattca ctcttgtcca gcgtaacgcc ggaatactct tccaccatgg aagcgaaacc
    67201 ccatgacatc gggcgtccgc agaaggcagc aaggatttgc gtgtcaatca agggttgtgg
    67261 taattcgcca aagacattga ggaacacttc cagatcttca ctgcctgcat ggagaaattt
    67321 tgtgatggac ggatcgcgca ggatcgcttt cagcggtgac cagtcggtga tcccgagagg
    67381 atcaattagc gccagatgct cgccatcgaa aagttgaatc aaccccagct gcgggtaata
    67441 agtgcgcgta cgaacaaatt cagtatccag ggctatcgcc ggaaaggcac ggacggcttc
    67501 acacaaagaa gccagcgcat cgtccgtggt aatcatttgg taattcaaat tgttttctct
    67561 ttagtgggcg tcaaaaaaaa cgccggatta accggcgtct gacgactgac ttaacactca
    67621 ggctttattg tccactttgc cgcgcgcttc gtcacgtaat tctcgtcgca aaatttttcc
    67681 gacgttagat ttcggtaact catcacgaaa ctccaccagc ttcggtactt tgtagcccgt
    67741 gagctgacgg cggcaaaagg tcaccagtga ctcttcggta agcgatggat cttttttcac
    67801 tacgaagatt ttcaccgctt caccactgga gccggaaggt acgccaacag ccgcgacttc
    67861 ctgtacgcca ggatgctgca tgacgacatc ttcaatctcg ttgggataga cgttaaaacc
    67921 ggaaaccaga atcatgtctt ttttacgatc gacaatgcgc aggaaccctt cttcatccat
    67981 caccgcgatg tcgccggtgt gtaaccagcc atttttgatg atctcatctg tagcatccgg
    68041 acgctgccag taacccagca tcacctgcgg tcctttgaca caaagctcac ccggttgacc
    68101 cggtggtact tcattatcat catcatccac cagtttggct tccgtcgacg gcaccggcaa
    68161 accgatgcta ccactatgat aatcaatatc atatgggtta acgctgacca gcggcgcaca
    68221 ctcggtaagg ccatagcctt ccagcagata ctgtcctgtc agtttcaccc aacgctctgc
    68281 caccacttgc tgcactggca tccctccgcc tgcggaaaga tgcagactgg agaaatccag
    68341 ctgctggaac tctttattgt tcagcaacgc attgaacaag gtgttaacgc ccgtgatagc
    68401 ggtaaacgga tatttcgcta actcttttac caaccctgga atatcgcgcg ggttagtgat
    68461 aagcaggttc tgcccaccca gttcgataaa cagcaggcag ttaatggtca gggcaaaaat
    68521 gtgatacagc ggcagcgccg tcaccaccag ctctttgccc ggatgcaaca gcggaccata
    68581 ggtcgcgtta acctgttcca ggttcgccag catattgcgg tgagtcagca tcgcgccttt
    68641 cgccacacca gtggtgccgc cggtgtattg cagaaaagct aaatcttccg gcaccagttc
    68701 gggtttgacg tactgcatcc ggtagccgtt atgcagtgcg ctacgaaatg aaatggcatc
    68761 tggcagatgg tatttcggca ccaaacgctt gatgtattta acaacgaaat tgactaccgt
    68821 gccttttgcc gtagatagct gatcgcccat acgggtcaga attacgtgct gaacggcggt
    68881 tttatcaacc actttttcca gtgtgtgagc aaagttagac acgataacaa tcgccgatgc
    68941 gccgctatcg ttaagctgat gctcaagctc acgcggggta tacaacgggt taacgtttac
    69001 gacgatcatc cctgcacgca aaatgccaaa cagcgccacc ggatattgca ataaattagg
    69061 catcatcaac gcaacgcgat cgcctttctt cagccccaac ccttgttgca aataagcggc
    69121 aaacgcgcga ctgcgttctt ccagcttgcg gaaggtcatt acctccccca tattcacaaa
    69181 cgcaggctga tcggcgtagc gcgcgaccga ctgctcaaac atatctacca gagattgata
    69241 acggtcaggg ttgatctccg tcggaacgtc cgcgggataa cggttaagcc aaaccttctt
    69301 caattcttca cctctaaaat gcgtgttcgt cgtcatcgca accccaaatg atatacatgc
    69361 cgttaacata atattaactc atcataccag cttgataatt acccaacgaa aaggttgcga
    69421 agcgcgtcac tatttatttt tatctttacc gtaagaatgc agaaacagcg gaccagccgc
    69481 tgtttctttt tctttaaaaa caagcaatta ttcagttaca actgtttgta ctctcgcggg
    69541 accgggatta taccagcccc atccgccgta gccatagggc cagccacgac cgccataaaa
    69601 ccatggatca atcggctgag gcggcataat cacctgctgg gttaaatgcc aacgtttgta
    69661 acccgttact tgcatcacca taaatttata gggcgtattg ccgattttgc cgtcaaccgc
    69721 accagtgatt ggcccaacta ccgtaaccag ttgtccacgg aaatccaccg ggtccagaaa
    69781 accgttcaca tcggcataaa tgcgaccgcg agaaggttct cccagcgtcg gtctggctcc
    69841 gctgtccagc ggtacggtag caatttccag gcgggttttc ccttgctggt tttgtaccgc
    69901 aaccactttg ccaccaaagc gtgcctcctg accaacgtac agctgcggcg cactcatcac
    69961 ccgaactaaa tcttgttgcg gcgtgggact gctgccttta atggcgtccg gcacagtgac
    70021 acaaccgctc agcattagcg caaacgtacc tgccagtatg cctttgataa cattcttttg
    70081 aaccgccatg atgcgactcc tttttttctc agggcatact cttaagattc attctttgcc
    70141 cggaagtttc ttccatgcga cgttgttacg taaataaacc ggttcggcat gttccaccgc
    70201 caccgtttta ccctcggcaa acatctgaca cgcaatcggc agcatatctt ccgccgctgg
    70261 cagtaaaact tctccgtcgc gcaaaaccag cccgctctct ttaccgagat ccggccaggc
    70321 ttgccagccc gtccctaccg ttacccattc gccggaaagc tgttgcattc gttcatgaac
    70381 gagttcgggt ttgagtacgg cttcggtttc ttcaccgtgc cagataccgt tttcatcacg
    70441 ctgatattcg gcccagtaaa cttcgcccat tcgcgcgtca atggctgcca gcacgcgggt
    70501 tgcgccgttt ttgcgccacg ccccttgcgc catcgtcatt agcgtggaga cgccaatcat
    70561 cggtagctct gcgccaagtg ccagcccttg cgcaatgcca ataccaatgc gcacgccggt
    70621 aaagctaccg gggccgcgcc cgtaagccag agcgttaata tcagtcaggg aagttccgct
    70681 ggtggtcagg atatcctgca ccatcggtaa gattcgttga gtatgttcac gagggcaaag
    70741 ctcaaaatga gcgttgacag taccgtcgtt ccacagggcg acagagcagg cctctgtcgc
    70801 ggtatcgata gccagaattc gcatgggtct tcgtgcttag atcaataaaa aggcgcgcat
    70861 cataccatac tccgtaacaa attacctgga ggatggtatc gcaaggaaac gaaccgcacg
    70921 ggcaatgtca cgagtgcgtg gcgcgggcgg cagactggcg agaaacgtcg cgccgtaagg
    70981 acgcatcacc agccgattgt cacaaatcac cagcacgcca cgatcgtcgg cgtcgcgaat
    71041 cagtcgccct accccctgtt tgagagtaat gacggcatct ggtagttgca cttcatcgaa
    71101 cgggtcgcca ccgcgcaaac gacaatcttc catgcgcgct tttaacagtg gatcatccgg
    71161 cgaggtaaac ggcaacttgt cgataatcac caatgacaat gtatcgccac gcacgtccac
    71221 cccttcccag aaactactgg ttgccacaag aagcgcgtta ccggcgctga caaattgctg
    71281 caacagttgc cctttgctgg tttccccctg caacaaaacg ggaagcgtca tggtagcgcg
    71341 gaactgctcg gccagatcac gcatcatggc gtgcgaggta caaagcataa aacaacgacc
    71401 gttgttagct tcgatgatcg gtcgcagcat tgccgctaac tggcgagcag aacctggctg
    71461 gttggtttgc ggcagattgc gcggcacaca gagtaacgcc tggcggctgt aatcaaacgg
    71521 gcttggcaat agcaacgact ccgcctgttc tatcccaagc cgcgaggtga aatgatgcag
    71581 atcgtcgttc accgacagcg ttgctgaggt gaagatccag ctaccaggct tttgcgccat
    71641 taactctttg aatttatccg ccacgctgag cggcgtgaga gccagagtaa aatggcgcga
    71701 agtgcattcg taccagtagc tgtagcccgg ctgattgatc tcttttagcc gcttcagccg
    71761 tgtgcgatac aacgtagcgc gctcaaatgc cgcatccagc aaggcggaac gccccagtga
    71821 cagtttcgcc acgtcataac aaagttccag ggtgtcatcg agcagtaaaa atgcccgctg
    71881 aatttgcgga ttagctaaca gctcacgcag gttaccgcga taacctggct caccgagttg
    71941 cagacgaaaa tcctgcgcac tctgggcaag acgatcagcg cacttttgta actgctgggt
    72001 gtcttttaat tcggtgcggt aagcgatggt gatgtctttt gccaggtcga gcagttgtcg
    72061 actggagagt gactgaccaa aatactggat ggcaatgtcc ggtagctggt gggcttcgtc
    72121 gaagatcatg acgtccgctt ccgggatcaa ctcgccaaat ccactctctt taaccaccat
    72181 atccgccaga aagagatgat ggtttaccac caccacatcg gcgtccatcg cttttttacg
    72241 tgctttgacc acaaagcaat ctttatacat cgggcagtcg ctgccaagac agttgtcgtt
    72301 ggtgctggta accagcggcc acgcctgtga atcttccgcc acgctgacgc aggtgctgat
    72361 atcaccatcg actgtttgat tagaccagga gcgcagcagg atcacatcgc ttaagatttg
    72421 taccggcaga tcgccccccg ccagcgcctg ctgttcgaga cgttcgaggc agaggtagtt
    72481 tgagcgccct ttcagcagcg ccacgttgcc cgtatatttc aatgcctttg agactgttgg
    72541 caaatcgcgg ctgtggagct gatcctgcaa cgcttttgag ccggtcgaga taatgacttt
    72601 ctttttcgcc cgcagcgcag gagccaggta agcgtaggtt ttgcccgtac cggttcctgc
    72661 ttccaccacc agcggctggc ctttttctat cgcctgggtg acggctaccg ccatctgtcg
    72721 ctgtggttct cgcggcttaa agcctggtat cgctttcgcc agctgaccgt ctggtgcaaa
    72781 atcgtccgtc acactacccc ctgttgattt gaacagggat tatgtcagga tgagggcgct
    72841 ttcgccagtt gaagtggtga cggcaacctc acattgtggc agtctttgca gcacgaaaat
    72901 ggaagtataa tgaggacaaa atgatgacta tcgttcgtat cgatgctgaa gcccgctggt
    72961 ctgatgtagt aatccacaac aacacgctct actacactgg tgtaccggaa aacctcgacg
    73021 ccgatgcttt tgagcaaacc gccaacacgc tggcgcagat tgacgccgtg ctggaaaaac
    73081 agggcagcaa taaatcgagc attctggatg ccaccatttt cctggctgat aaaaacgact
    73141 tcgcggcgat gaataaagcg tgggatgcct gggttgtggc gggtcatgcg ccggtgcgct
    73201 gcacggtgca agcggggctg atgaacccga agtataaagt tgaaattaag attgtggctg
    73261 cggtgtaagc tttatcgaag caaaataagt cagacgataa tttatcgata atactggtcg
    73321 gttttacata aatcgaccag agattaagat attactgttg atgctcctgc taattcatta
    73381 tttattttta ta
//
LOCUS       DAZFXT010000009        71708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.009, whole genome shotgun sequence.
ACCESSION   DAZFXT010000009 DAZFXT010000000
VERSION     DAZFXT010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.009"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            98..1330
                     /gene="dgcM"
                     /locus_tag="ACHKW0_000653"
     CDS             98..1330
                     /gene="dgcM"
                     /locus_tag="ACHKW0_000653"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HFO7473537.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFVHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(1351..1914)
                     /gene="smrA"
                     /locus_tag="ACHKW0_000654"
     CDS             complement(1351..1914)
                     /gene="smrA"
                     /locus_tag="ACHKW0_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HFO7473538.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(2244..3152)
                     /gene="abgR"
                     /locus_tag="ACHKW0_000655"
     CDS             complement(2244..3152)
                     /gene="abgR"
                     /locus_tag="ACHKW0_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7473539.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            3328..4638
                     /gene="abgA"
                     /locus_tag="ACHKW0_000656"
     CDS             3328..4638
                     /gene="abgA"
                     /locus_tag="ACHKW0_000656"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HFO7473540.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGM
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            4638..6083
                     /gene="abgB"
                     /locus_tag="ACHKW0_000657"
     CDS             4638..6083
                     /gene="abgB"
                     /locus_tag="ACHKW0_000657"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HFO7473541.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESASFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWCFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            6117..7643
                     /gene="abgT"
                     /locus_tag="ACHKW0_000658"
     CDS             6117..7643
                     /gene="abgT"
                     /locus_tag="ACHKW0_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HFO7473542.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            7654..8169
                     /gene="ogt"
                     /locus_tag="ACHKW0_000659"
     CDS             7654..8169
                     /gene="ogt"
                     /locus_tag="ACHKW0_000659"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HFO7473543.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            8364..9116
                     /gene="fnr"
                     /locus_tag="ACHKW0_000660"
     CDS             8364..9116
                     /gene="fnr"
                     /locus_tag="ACHKW0_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HFO7473544.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            9268..10218
                     /gene="uspE"
                     /locus_tag="ACHKW0_000661"
     CDS             9268..10218
                     /gene="uspE"
                     /locus_tag="ACHKW0_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HFO7473545.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(10268..10525)
                     /gene="ynaJ"
                     /locus_tag="ACHKW0_000662"
     CDS             complement(10268..10525)
                     /gene="ynaJ"
                     /locus_tag="ACHKW0_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HFO7473546.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            10769..11800
                     /gene="ynaI"
                     /locus_tag="ACHKW0_000663"
     CDS             10769..11800
                     /gene="ynaI"
                     /locus_tag="ACHKW0_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HFO7473547.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSIINYVIENYKLKFITPGVIDFICTSLIAVILTIKLFLLINQFE
                     KQQIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAG
                     KDILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPN
                     SLFSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLV
                     YFNQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMD
                     NITPQEQGR"
     gene            complement(11851..13464)
                     /gene="mppA"
                     /locus_tag="ACHKW0_000664"
     CDS             complement(11851..13464)
                     /gene="mppA"
                     /locus_tag="ACHKW0_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HFO7473548.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(13801..14700)
                     /gene="pgrR"
                     /locus_tag="ACHKW0_000665"
     CDS             complement(13801..14700)
                     /gene="pgrR"
                     /locus_tag="ACHKW0_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HFO7473549.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSAMASLSDLQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            14838..15758
                     /locus_tag="ACHKW0_000666"
     CDS             14838..15758
                     /locus_tag="ACHKW0_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7473550.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVLSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            15758..16690
                     /locus_tag="ACHKW0_000667"
     CDS             15758..16690
                     /locus_tag="ACHKW0_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7473551.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTFGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKAHGVTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            16708..17346
                     /locus_tag="ACHKW0_000668"
     CDS             16708..17346
                     /locus_tag="ACHKW0_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309933.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7473552.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKSFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGDNIGINQPVTDGDKPLFM"
     gene            17655..18383
                     /gene="mpaA"
                     /locus_tag="ACHKW0_000669"
     CDS             17655..18383
                     /gene="mpaA"
                     /locus_tag="ACHKW0_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HFO7473553.1"
                     /translation="MTVNRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(18358..19323)
                     /gene="ycjG"
                     /locus_tag="ACHKW0_000670"
     CDS             complement(18358..19323)
                     /gene="ycjG"
                     /locus_tag="ACHKW0_000670"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HFO7473554.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            19442..19948
                     /gene="tpx"
                     /locus_tag="ACHKW0_000671"
     CDS             19442..19948
                     /gene="tpx"
                     /locus_tag="ACHKW0_000671"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HFO7473555.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(19992..21533)
                     /gene="tyrR"
                     /locus_tag="ACHKW0_000672"
     CDS             complement(19992..21533)
                     /gene="tyrR"
                     /locus_tag="ACHKW0_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HFO7473556.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFAQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(21681..22742)
                     /gene="ycjF"
                     /locus_tag="ACHKW0_000673"
     CDS             complement(21681..22742)
                     /gene="ycjF"
                     /locus_tag="ACHKW0_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjF family protein"
                     /protein_id="HFO7473557.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGTVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(22739..24136)
                     /gene="ycjX"
                     /locus_tag="ACHKW0_000674"
     CDS             complement(22739..24136)
                     /gene="ycjX"
                     /locus_tag="ACHKW0_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HFO7473558.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            24291..25289
                     /gene="ycjW"
                     /locus_tag="ACHKW0_000675"
     CDS             24291..25289
                     /gene="ycjW"
                     /locus_tag="ACHKW0_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7473559.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(25400..26305)
                     /gene="ompG"
                     /locus_tag="ACHKW0_000676"
     CDS             complement(25400..26305)
                     /gene="ompG"
                     /locus_tag="ACHKW0_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HFO7473560.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(26350..27432)
                     /locus_tag="ACHKW0_000677"
     CDS             complement(26350..27432)
                     /locus_tag="ACHKW0_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7473561.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(27446..28105)
                     /gene="pgmB"
                     /locus_tag="ACHKW0_000678"
     CDS             complement(27446..28105)
                     /gene="pgmB"
                     /locus_tag="ACHKW0_000678"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008801 - beta-phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HFO7473562.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(28102..30369)
                     /gene="ycjT"
                     /locus_tag="ACHKW0_000679"
     CDS             complement(28102..30369)
                     /gene="ycjT"
                     /locus_tag="ACHKW0_000679"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HFO7473563.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(30366..31421)
                     /gene="ycjS"
                     /locus_tag="ACHKW0_000680"
     CDS             complement(30366..31421)
                     /gene="ycjS"
                     /locus_tag="ACHKW0_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7473564.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALADKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRELADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(31431..32219)
                     /gene="ycjR"
                     /locus_tag="ACHKW0_000681"
     CDS             complement(31431..32219)
                     /gene="ycjR"
                     /locus_tag="ACHKW0_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7473565.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLHVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(32237..33289)
                     /gene="ycjQ"
                     /locus_tag="ACHKW0_000682"
     CDS             complement(32237..33289)
                     /gene="ycjQ"
                     /locus_tag="ACHKW0_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HFO7473566.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFAKSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(33320..34162)
                     /gene="ycjP"
                     /locus_tag="ACHKW0_000683"
     CDS             complement(33320..34162)
                     /gene="ycjP"
                     /locus_tag="ACHKW0_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7473567.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(34149..35030)
                     /gene="ycjO"
                     /locus_tag="ACHKW0_000684"
     CDS             complement(34149..35030)
                     /gene="ycjO"
                     /locus_tag="ACHKW0_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HFO7473568.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     HLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(35051..36343)
                     /gene="ycjN"
                     /locus_tag="ACHKW0_000685"
     CDS             complement(35051..36343)
                     /gene="ycjN"
                     /locus_tag="ACHKW0_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7473569.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILLAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(36357..38036)
                     /gene="ycjM"
                     /locus_tag="ACHKW0_000686"
     CDS             complement(36357..38036)
                     /gene="ycjM"
                     /locus_tag="ACHKW0_000686"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HFO7473570.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGKSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFYPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(38249..38563)
                     /gene="pspE"
                     /locus_tag="ACHKW0_000687"
     CDS             complement(38249..38563)
                     /gene="pspE"
                     /locus_tag="ACHKW0_000687"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HFO7473571.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(38638..38859)
                     /gene="pspD"
                     /locus_tag="ACHKW0_000688"
     CDS             complement(38638..38859)
                     /gene="pspD"
                     /locus_tag="ACHKW0_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HFO7473572.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(38868..39227)
                     /gene="pspC"
                     /locus_tag="ACHKW0_000689"
     CDS             complement(38868..39227)
                     /gene="pspC"
                     /locus_tag="ACHKW0_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HFO7473573.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPAKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(39227..39451)
                     /gene="pspB"
                     /locus_tag="ACHKW0_000690"
     CDS             complement(39227..39451)
                     /gene="pspB"
                     /locus_tag="ACHKW0_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009271 - phage shock [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HFO7473574.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(39505..40173)
                     /gene="pspA"
                     /locus_tag="ACHKW0_000691"
     CDS             complement(39505..40173)
                     /gene="pspA"
                     /locus_tag="ACHKW0_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HFO7473575.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            40340..41317
                     /gene="pspF"
                     /locus_tag="ACHKW0_000692"
     CDS             40340..41317
                     /gene="pspF"
                     /locus_tag="ACHKW0_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HFO7473576.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(41437..42702)
                     /gene="puuE"
                     /locus_tag="ACHKW0_000693"
     CDS             complement(41437..42702)
                     /gene="puuE"
                     /locus_tag="ACHKW0_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HFO7473577.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGKPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDVQFDAAMKILQDALSD"
     gene            complement(42740..44020)
                     /gene="puuB"
                     /locus_tag="ACHKW0_000694"
     CDS             complement(42740..44020)
                     /gene="puuB"
                     /locus_tag="ACHKW0_000694"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HFO7473578.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESISCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(44022..45509)
                     /gene="puuC"
                     /locus_tag="ACHKW0_000695"
     CDS             complement(44022..45509)
                     /gene="puuC"
                     /locus_tag="ACHKW0_000695"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HFO7473579.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(45784..46341)
                     /gene="puuR"
                     /locus_tag="ACHKW0_000696"
     CDS             complement(45784..46341)
                     /gene="puuR"
                     /locus_tag="ACHKW0_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HFO7473580.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(46368..47120)
                     /gene="puuD"
                     /locus_tag="ACHKW0_000697"
     CDS             complement(46368..47120)
                     /gene="puuD"
                     /locus_tag="ACHKW0_000697"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HFO7473581.1"
                     /translation="MNNPVIGVVMCRNRLKDHATQTLQEKYLNAIIHAGGLPIALPHA
                     LAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINAALER
                     RIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQVEEG
                     GLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALGVQWH
                     PEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            47344..48762
                     /gene="puuA"
                     /locus_tag="ACHKW0_000698"
     CDS             47344..48762
                     /gene="puuA"
                     /locus_tag="ACHKW0_000698"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HFO7473582.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            48911..49075
                     /locus_tag="ACHKW0_000699"
     CDS             48911..49075
                     /locus_tag="ACHKW0_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473583.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            49065..50450
                     /gene="puuP"
                     /locus_tag="ACHKW0_000700"
     CDS             49065..50450
                     /gene="puuP"
                     /locus_tag="ACHKW0_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HFO7473584.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            50584..50829
                     /gene="ymjA"
                     /locus_tag="ACHKW0_000701"
     CDS             50584..50829
                     /gene="ymjA"
                     /locus_tag="ACHKW0_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HFO7473585.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            51142..52785
                     /gene="sapA"
                     /locus_tag="ACHKW0_000702"
     CDS             51142..52785
                     /gene="sapA"
                     /locus_tag="ACHKW0_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein SapA"
                     /protein_id="HFO7473586.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            52782..53747
                     /gene="sapB"
                     /locus_tag="ACHKW0_000703"
     CDS             52782..53747
                     /gene="sapB"
                     /locus_tag="ACHKW0_000703"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine export ABC transporter permease SapB"
                     /protein_id="HFO7473587.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            53734..54624
                     /gene="sapC"
                     /locus_tag="ACHKW0_000704"
     CDS             53734..54624
                     /gene="sapC"
                     /locus_tag="ACHKW0_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HFO7473588.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            54624..55616
                     /gene="sapD"
                     /locus_tag="ACHKW0_000705"
     CDS             54624..55616
                     /gene="sapD"
                     /locus_tag="ACHKW0_000705"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HFO7473589.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            55618..56424
                     /gene="sapF"
                     /locus_tag="ACHKW0_000706"
     CDS             55618..56424
                     /gene="sapF"
                     /locus_tag="ACHKW0_000706"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HFO7473590.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            56492..56845
                     /gene="ycjD"
                     /locus_tag="ACHKW0_000707"
     CDS             56492..56845
                     /gene="ycjD"
                     /locus_tag="ACHKW0_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HFO7473591.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQNWTVLRFWHNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            57213..58001
                     /gene="fabI"
                     /locus_tag="ACHKW0_000708"
     CDS             57213..58001
                     /gene="fabI"
                     /locus_tag="ACHKW0_000708"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HFO7473592.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            58146..59273
                     /gene="yciW"
                     /locus_tag="ACHKW0_000709"
     CDS             58146..59273
                     /gene="yciW"
                     /locus_tag="ACHKW0_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HFO7473593.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            59341..61275
                     /gene="rnb"
                     /locus_tag="ACHKW0_000710"
     CDS             59341..61275
                     /gene="rnb"
                     /locus_tag="ACHKW0_000710"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008859 - exoribonuclease II activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HFO7473594.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(61353..61457)
                     /locus_tag="ACHKW0_000711"
     CDS             complement(61353..61457)
                     /locus_tag="ACHKW0_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473595.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            61511..63496
                     /gene="pdeR"
                     /locus_tag="ACHKW0_000712"
     CDS             61511..63496
                     /gene="pdeR"
                     /locus_tag="ACHKW0_000712"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7473596.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTCKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            complement(63478..63606)
                     /gene="ymiD"
                     /locus_tag="ACHKW0_000713"
     CDS             complement(63478..63606)
                     /gene="ymiD"
                     /locus_tag="ACHKW0_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmiD"
                     /protein_id="HFO7473597.1"
                     /translation="MQKLPLKEKCLTATANYHPGIRYIMTGYSAKYIYSSTYARFR"
     gene            63643..63816
                     /gene="yciZ"
                     /locus_tag="ACHKW0_000714"
     CDS             63643..63816
                     /gene="yciZ"
                     /locus_tag="ACHKW0_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciZ"
                     /protein_id="HFO7473598.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            63906..64655
                     /gene="yciT"
                     /locus_tag="ACHKW0_000715"
     CDS             63906..64655
                     /gene="yciT"
                     /locus_tag="ACHKW0_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YciT"
                     /protein_id="HFO7473599.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            64924..65142
                     /gene="osmB"
                     /locus_tag="ACHKW0_000716"
     CDS             64924..65142
                     /gene="osmB"
                     /locus_tag="ACHKW0_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HFO7473600.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(65268..65594)
                     /gene="yciH"
                     /locus_tag="ACHKW0_000717"
     CDS             complement(65268..65594)
                     /gene="yciH"
                     /locus_tag="ACHKW0_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HFO7473601.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(65594..66331)
                     /gene="pyrF"
                     /locus_tag="ACHKW0_000718"
     CDS             complement(65594..66331)
                     /gene="pyrF"
                     /locus_tag="ACHKW0_000718"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HFO7473602.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CSLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(66524..67693)
                     /gene="lapB"
                     /locus_tag="ACHKW0_000719"
     CDS             complement(66524..67693)
                     /gene="lapB"
                     /locus_tag="ACHKW0_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0008653 - lipopolysaccharide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HFO7473603.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQIYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(67700..68008)
                     /gene="lapA"
                     /locus_tag="ACHKW0_000720"
     CDS             complement(67700..68008)
                     /gene="lapA"
                     /locus_tag="ACHKW0_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HFO7473604.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(68157..68921)
                     /gene="pgpB"
                     /locus_tag="ACHKW0_000721"
     CDS             complement(68157..68921)
                     /gene="pgpB"
                     /locus_tag="ACHKW0_000721"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HFO7473605.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            69091..69681
                     /gene="ribA"
                     /locus_tag="ACHKW0_000722"
     CDS             69091..69681
                     /gene="ribA"
                     /locus_tag="ACHKW0_000722"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HFO7473606.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(69745..>71708)
                     /gene="acnA"
                     /locus_tag="ACHKW0_000723"
     CDS             complement(69745..>71708)
                     /gene="acnA"
                     /locus_tag="ACHKW0_000723"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HFO7473607.1"
                     /translation="GQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGVVG
                     KFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVELV
                     EKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKAFAASNE
                     LEVNATHKNRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKAVT
                     LGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNSGPLPDP
                     IETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAAEP
                     IGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRSDT
                     YGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPDSP
                     AGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPDSD
                     VVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIHRS
                     NLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEVVP
                     CRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        17378 a        18664 c        17735 g        17931 t
ORIGIN      
        1 taaatagcgt ctatcattat cagaattatc tgatcatatg acgtggcttt tttgcgatcg
       61 gatagcaaca aaaattgata aaaataacgg gatctcaatg attacgcaca acttcaatac
      121 cctggactta ctcaccagtc ctgtctggat tgtttcgccc tttgaggaac agttaattta
      181 tgccaatagc gcggcgcgac tgttgatgca agacctcacg tttagtcagc tacgaaccgg
      241 accctattcc gtctcctcac aaaaagaact gccgaaatac ctctccgatc tgcaaaacca
      301 acacgatatt atcgaaatcc tcactgttca gcgtaaagaa gaggaaacag cattaagctg
      361 tcggcttgtt ttgcgagagc tgacagaaac agaaccggtg attattttcg aaggtatcga
      421 agcgccggca acgctgggtt taaaagccag tcgctcggca aattatcagc gcaaaaaaca
      481 aggtttttat gcgcgctttt ttctgactaa ctctgcacca atgttgttga ttgacccctc
      541 acgagatggc caaatcgtcg atgctaacct cgccgcgctc aatttctatg gttataacca
      601 tgaaacgatg tgccagaaac atacctggga aattaatatg ctcgggcgtc gcgtcatgcc
      661 tatcatgcat gaaatttcgc atttacccgg cggtcataaa cctttgaatt ttgttcataa
      721 actggcggat ggatctactc gtcatgtgca gacctatgcc gggccgattg aaatttatgg
      781 cgacaagctc atgttatgta ttgtgcatga tattactgag caaaaacggc tggaggagca
      841 gctggaacat gctgctcacc atgacgcgat gaccggatta ctgaatcggc gacagtttta
      901 tcacattacc gagccaggcc aaatgcagca tctcgccatc gctcaggatt acagcttgtt
      961 gctcatcgac accgatcgtt ttaaacacat taacgatctc tatgggcatt ctaaaggtga
     1021 tgaggtgtta tgcgccctcg cccgcaccct tgaaagttgc gctcgcaaag gcgatttggt
     1081 gtttcgttgg ggaggcgaag agtttgtctt attgctacca agaaccccac tggataccgc
     1141 gctttcgctg gctgaaacta tccgtgtaag cgtggcaaaa gtgagtattt cgggcttacc
     1201 acgctttacc gtcagcattg gtgtggcgca tcacgaagga aatgaaagca tcgatgaact
     1261 gtttaaacgc gttgatgatg ctttgtatcg ggcgaaaaat gatggacgca accgcgtgct
     1321 ggcggcataa gccgcggatg cgtctcgaga tcaacgactg cgcttagcgt ggcgctccca
     1381 gttttcttgc ttcgcctgcg ccgttttacg tagtgcgacg taacacgccc cgctgccgcc
     1441 atgatgcggt agcgcagtgc aatatgcctg aacatcatca aattcggtca gccagcgcgc
     1501 cacatagctg cggacaatat tggcatgcga tttatcatcc cgtcctttgc catgaataat
     1561 cagcacgtta cgcaaaccat cagccatggc ttgttgaatg aaactgaaca tcattttgcg
     1621 gcactcttcc accggctggc gcaaaagatt caggctcgcc tgttgcggat atttaccact
     1681 gcgcagctta tccagcaccc catgttgcaa cccttcccgc cgaaactcca gcggctgact
     1741 tagtgggatg atgtcgagaa atccggtggt gaggaaatta tcaagctgca gcgtgtcgat
     1801 acgctgcggc gcacgttggt tacgcgttgg atgccagtgg acatcggtag cacgtttcag
     1861 cggctggaca tcttccatgg cgtcaagaaa cagcgatttg tcgtcaaggt tcatgttaca
     1921 tcctcccgca attaagagcg cgatatgata accagaccgg gtcggtccaa caacgtatta
     1981 cccaaaattc cagtaataag ttccaaatat tgccgatatt ttaagcaaaa tgcttatgca
     2041 tgattattca ttcacgatat taataatgta acttatattt tcgtgaaatc tgtcactgaa
     2101 gaaaattggc aactaaaggt taaaaccgtt ataacacagt caccggcgca gaggagacaa
     2161 tgccggactt aagacgcgga tgcactgctg tgtgtactgt agagtctggc ggatgtcgac
     2221 agactctatt tttttatgca gttttaactt tgcagatagc cgcattctcg ccggaattgc
     2281 gtgattaatg acgcggtcag tggtgtctgg cgactatcac gccgctgaat caaataatag
     2341 gccgctttcg gtaaaatttc gctaaccggc aacatcacca gcccctgtcc gtgcaaggga
     2401 tcgcagccca tttcttcagg cagtatgctg agaaaatcac tttttgccac cagactgata
     2461 caggctgaga acgtctcgca gactacaccg acctgtggcg tttgcgcctg atcgtcaagc
     2521 aattcactca actgtttgta gtaactgccg tgtggcgtcg gcattgtcca gctgtaatcc
     2581 agtaactgtt tgatcgaacg ggcaccaatg gcggggtgtc ccgggcggca aaagatcgcg
     2641 aattgctttt ccagtaattt ctcaaaagta aattcgtggt cgtacggtcc ctgataatag
     2701 gtattgatgg tgaaatccaa ttctccctga cgcaattcat taatcatcga caccagttgc
     2761 ccttccataa tgcgtacttt tacctgcgga tgctgctgat gaaaacgaga tatgacagct
     2821 ggcatcagac tgcgggaaat actggccccc atgccgatat taatctgccc tgccagttgc
     2881 ccttgtcgtt ggcgaatttc ctcttgcgct gcgcgcagct cttcaagaat aagactggcg
     2941 tgctgataaa aactttcacc ggcatcagtt aacgtcacgc ctttactacg gcgaaaaaag
     3001 agttgcgccg ctaatccttc ttccagctcc tgaatagatt tactcagtgc cggttgcgac
     3061 atattcaaca ttcggctcgc tccgcgaatg ctgccctgac gagccacttc aacaaaagca
     3121 cgaatttgat gaatttttac ctgaaaagcc atgacgccac cgataaccgt tatttatcag
     3181 accaaagaaa ctggcatcta ctttaatgca gatgattgtg tcagggtaat ttatgaacgg
     3241 ttaaaactgt gaaaaatcag ttagtgataa gtaaaaacta tcgctacatg aaccgggtca
     3301 cactttttac tgatgacggg aaaggttatg gagtctttga atcaatttgt taattcgctt
     3361 gccccaaaat tatcgcactg gcgacgtgat tttcatcact atgcagagtc tggctgggtg
     3421 gaattccgca ctgccaccct tgttgcggaa gaattgcagc agctcggcta ttcactggcg
     3481 ctgggccgcg aagtagttaa tgaaagtagc cggatgggac tacctgatga actcactcta
     3541 caacgcgaat tcgagcgcgc tcgtcaacag ggtgcgctag cacaatggat tgcggttttt
     3601 gaaggtggtt tcactggtat cgtcgccacc ctggataccg gtcgccccgg tccggtgatg
     3661 gctttccgtg tcgatatgga cgcgctggat ctcagtgaag agcaggatgt cagccatcgc
     3721 ccctaccgcg acggttttgc gtcatgtaac gccggaatga tgcatgcctg tggtcatgat
     3781 ggacataccg ccattgggct tgggatggcg cataccctta aacaattcga gtccggacta
     3841 catggcgtca tcaaactgat ttttcagcct gcagaggaag gtacgcgtgg cgcgcgggcg
     3901 atggtcgatg caggtgtcgt agatgatgtt gattatttta ctgccgtgca cattggcact
     3961 ggcgtacctg cgggcaccgt ggtgtgcggc agtgataatt ttatggcaac caccaaattt
     4021 gacgcgcact tcaccgggac cgccgctcac gcaggcgcaa aaccagaaga cggtcacaat
     4081 gccttgttgg cggcagcaca agcaactctt gcactgcatg caatcgcccc gcacagcgaa
     4141 ggagcttcca gagtaaacgt gggcgttatg caggcaggaa gcggccgtaa cgttgttcct
     4201 gcctcggcgt tgctgaaagt ggaaacacgc ggggccagcg acgtcattaa tcaatatgtt
     4261 tttgaccgtg cacaacaagc gattcagggc gcagcaacca tgtatggtgt cggcgttgaa
     4321 actcgtctga tgggtgcagc taccgccagt tctccttcgc cgcaatgggt cgcatggttg
     4381 caaagtcagg cggctcaggt cgcgggggtc aatcaggcca ttgaacgtgt tgaagcgcct
     4441 gcgggttccg aagatgccac attaatgatg gcccgcgtgc agcaacatca agggcaagcc
     4501 tcctacgtgg tgtttggcac acagctggcg gcaggtcatc acaacgaaaa attcgatttt
     4561 gacgagcagg ttctcgctat tgccgtcgaa acgctggcgc gcaccgcgct caattttccc
     4621 tggacgcgag gtatctgatg caggaaatct atcgttttat cgacgatgcg attgaagccg
     4681 atcgccaacg ttataccgat attgccgatc aaatctggga tcatccagaa acacgttttg
     4741 aagagttctg gtcagcggag catctggctt cggcgctgga atctgcaagc ttcaccgtta
     4801 cccgcaacgt aggcaatatc ccaaatgcat ttattgcttc gtttggtcaa ggcaaaccgg
     4861 ttatcgccct gctgggagaa tatgacgccc tggcaggttt aagtcagcaa gctggttgcg
     4921 cgcaacctac atccgcgacg cccggtgaaa atggtcacgg ttgcggacac aatttgctgg
     4981 gaaccgccgc ctttgccgct gcaatagccg tcaagaaatg gctggaacaa tatgggcaag
     5041 gcggcacagt gcgcttttat ggttgtcctg gcgaagaagg cggctcgggt aaaacgttca
     5101 tggtccgcga gggggtattt gatgatgtgg atgcggcact cacctggcac ccggaagcct
     5161 ttgccggcat gttcaatacc cgcacgctgg caaacattca ggcatcatgg tgctttaaag
     5221 ggatcgcagc acatgccgcg aattcccctc atttgggacg cagcgccctt gatgccgtaa
     5281 cgttgatgac cactggcacc aacttcctca acgaacatat tattgaaaaa gcgcgcgtac
     5341 actatgccat cacaaatagc ggcgggatct cgcccaacgt ggtccaggcg caggcagaag
     5401 tgctttatct tatccgtgcc cccgaaatga ccgacgtgca gcatatttat gatcgggtcg
     5461 ccaaaatcgc cgaaggtgcg gcattgatga ccgaaaccac ggttgaatgc cgcttcgaca
     5521 aagcctgttc cagttatctc ccgaatcgca ccttagaaaa tgccatgtac catgccctat
     5581 cccattttgg taccccggaa tggaactccg aagaactggc ttttgcgaaa caaattcagg
     5641 ctacgctcac ccccaacgat cggcaaaaca gtctgaataa tatcgccgca accggtggcg
     5701 aaaacggcaa ggtttttgca ctacgtcatc gtgaaacggt actggcgaat gaggtcgctc
     5761 catatgccgc caccgataac gtgcttgcgg catcgactga tgtcggcgac gtcagttgga
     5821 aactgcctgt tgcccagtgt ttcagcccct gttttgccgt cggtacaccg ctacatacgt
     5881 ggcaactggt tagccagggg cgaacatcta ttgctcataa aggaatgctg ctggcggcga
     5941 aaactatggc agcaaccaca gtcaatctct tcattgattc agggctattg caagaatgcc
     6001 aacaagagca tcagcaagtt acggacacgc aaccgtatca ctgccctatc ccgaaaaacg
     6061 tgacaccgtc acctttaaaa taacaacaac gcaaacacaa caaccgagga atgcccatga
     6121 gtatgtcatc cataccgtcg tcctcccaat ccgggaagct ctatggctgg gtcgaaagaa
     6181 ttggtaacaa ggttccccat ccttttttgc tctttatcta tttgataatc gtactcatgg
     6241 tgacgacggc aattttgtca gcctttggcg tcagtgcgaa aaacccgacc gatggtacgc
     6301 cggtggtggt gaaaaacctg ctcagtgtgg aaggattaca ctggttttta cccaatgtga
     6361 ttaaaaactt tagcggtttt gctccacttg gagcgatcct ggcgctggtt ttaggtgccg
     6421 gcctagcgga gcgcgtcggc ttactgccag cgctaatggt taaaatggca tcgcatgtta
     6481 atgcccgcta cgccagttat atggtgctgt ttattgcttt tttcagccac atttcttccg
     6541 atgcggcgtt agtgatcatg ccaccgatgg gtgcgctgat ttttctggcg gtgggcaggc
     6601 atccagttgc aggtttactg gctgccattg caggcgtagg ttgcggcttt acggctaatt
     6661 tactgattgt cacaaccgac gtgttgctgt cggggatcag cacggaagcg gcggctgcgt
     6721 tcaatccgca aatgcacgtc agtgtaattg ataactggta ttttatggcc agctccgtag
     6781 tcgtactgac gattgttggc ggcttgataa ccgacaaaat catcgagcca cggttaggtc
     6841 aatggcaggg aaacagcgat gagaaactgc agacattgac cgaaagtcag cgttttggtt
     6901 tacgcatagc aggtgtcgta tcgctacttt ttattgctgc gatcgcgctg atggtgatcc
     6961 cggaaaacgg gatattgcgc gatccgatta atcacaccgt gatgccatca ccctttatta
     7021 aaggtatcgt gccactaatc attctttttt tctttgtggt ttcgctggct tatggcatcg
     7081 ctacccgcac aattcgacgt caggcggatt taccgcattt aatgattgaa ccgatgaaag
     7141 agatggcggg atttatcgtg atggtttttc ccctcgccca gtttgtcgcc atgtttaact
     7201 ggagcaacat ggggaaattc atcgccgtgg ggctgaccga tatacttgaa agttcagggc
     7261 ttagcggcat cccggcgttt gtcggtctgg cgttgctttc ctctttctta tgcatgttta
     7321 ttgccagcgg ttccgcaatc tggtcgattc tggcccccat tttcgtacca atgtttatgc
     7381 tacttggctt tcacccggca tttgcgcaaa tcctctttcg tattgccgac tcatccgtat
     7441 tgcctttagc gccggtatct ccttttgttc cactgtttct tggattcctg caacgctaca
     7501 aaccagacgc gaaactgggt acttactatt cgttagtctt gccctatccg cttatctttt
     7561 tggtggtatg gctgctgatg ttgctggcgt ggtatcttgt tggtctgccg ataggtccgg
     7621 ggatttaccc acgtttgtct taagagagaa cggatgctga gattacttga agaaaaaatt
     7681 gccacgccac tgggtccact gtgggtgatt tgcgatgagc aatttcgcct gcgggcggtt
     7741 gaatgggaag agtacagcga acgcatggtg cagctgctgg acatccatta tcgcaaagaa
     7801 ggctatgagc gcatttctgc caccaaccca ggcggtttaa gcgacaagct tcgtgattat
     7861 tttgccggta atcttagcat tattgatacg cttcccactg ccacaggggg gacgccattt
     7921 cagcgcgaag tctggaaaac tctacgcact atcccctgcg ggcaggtaat gcattacggc
     7981 caactggctg aacaattggg ccgccctggc gcggcgcgtg ccgttggtgc ggcaaacgga
     8041 tcgaatccca tcagcatcgt cgtaccttgc catcgggtta ttggccgaaa cggcaccatg
     8101 accggatatg caggcggagt tcagcgaaaa gagtggttat tgcgccacga aggttatctt
     8161 ttgctgtaaa cattaaacaa tttgtgccag cttgttcaca cttttatgta aagttaccct
     8221 taacaactta agggttttca aatagataga catatattta catctaatat cggaattctc
     8281 tgctgttaag gtttgcttag acttacttgc tccctaaaaa gatgttaaaa ttgacaaata
     8341 tcaattacgg cttgagcaga cctatgatcc cggaaaagcg aattatacgg cgcattcagt
     8401 ctggcggttg tgctatccat tgccaggatt gcagcatcag ccagctttgc atcccgttca
     8461 cactcaacga acatgagctt gatcagcttg ataatatcat tgagcggaag aagcctattc
     8521 agaaaggcca gacgctgttt aaggctggtg atgaacttaa atcgctttat gccatccgct
     8581 ccggtacgat taaaagttat accatcactg agcaaggcga cgagcaaatc actggtttcc
     8641 atttagcagg cgacctggtg ggatttgacg ccatcggcag cggccatcac ccgagcttcg
     8701 cgcaggcgct ggaaacctcg atggtatgtg aaatcccgtt cgaaacgctg gacgatttgt
     8761 ctggtaaaat gccgaatctg cgtcagcaga tgatgcgtct gatgagcggt gaaatcaaag
     8821 gcgatcagga catgatcctg ctgttgtcga agaaaaatgc cgaggaacgt ctggctgcat
     8881 tcatctacaa cctgtcccgt cgttttgccc aacgcggctt ctcccctcgt gaattccgcc
     8941 tgacgatgac tcgtggcgat atcggtaact atctgggcct gacggtagaa accatcagcc
     9001 gtctgctggg tcgcttccag aaaagcggta tgctggcagt caaaggtaaa tacatcacca
     9061 tcgaaaataa cgatgcgctg gcccagcttg ctggtcatac gcgtaacgtt gcctgatttt
     9121 tccgcataac tcactatcct tctgtcatat cattaaattt ttctgattta ttgatctggc
     9181 agaaggttca tcactgtttc attcaccaga tatgggttaa tcttttaatt acaaactgcg
     9241 ttgacagttg ttgtaaggag accctgtatg gctatgtatc agaacatgct cgttgttatc
     9301 gatcctaacc aggacgacca accagcattg cggcgagctg tttatttaca tcaacggatt
     9361 ggtggcaaaa ttaaagcctt tttgccgatc tatgacttct catacgaaat gaccaccctg
     9421 ctctccccgg acgaacgtac cgctatgcgt cagggcgtca tcagccagcg tacagcctgg
     9481 atccacgagc aggcaaaata ttatctcaat gctggcgttc ccattgaaat taaagtggtc
     9541 tggcataacc gtcccttcga agccatcatt caggaagtga tcagcggcgg gcacgatttg
     9601 gtgctaaaaa tggcgcacca acatgaccgt ctggaagcgg tgatttttac gccaacggac
     9661 tggcatctgt tacgcaaatg cccaagcccg gtgtggatgg tgaaagacca gccgtggccg
     9721 gaaggaggca aggcgctggt ggcggtgaat ctcgccagtg aagagccgta tcataatgcg
     9781 ctcaatgaaa aactggtcaa agagacgatc gaactggcag aacaagtcaa ccataccgaa
     9841 gttcatctgg ttggcgctta tccggtaacg ccaattaata tcgcgattga actgccggaa
     9901 tttgatccga gcgtttataa cgatgccatt cgtgggcaac atttgctggc aatgaaagct
     9961 ctgcggcaga aattcggcat taatgaaaac atgacgcacg tagaaaaagg tctgccagaa
    10021 gaggtgattc ctgatttggc ggagcactta caggcgggga ttgtggttct gggcacggta
    10081 ggacgcaccg gtatttcagc agcattcctc ggcaacacgg cggaacaggt gattgatcat
    10141 cttcgctgcg atctgttggt cattaaacct gaccagtatc agacacccgt tgaactggat
    10201 gacgaagaag acgattaacg actcttcaat aactgacaag gcccgagtta tcgggcctat
    10261 tttttactta ctgctcttcg ccgccaagga aataaattcc caacgggatt gccagcaaca
    10321 cagtaaagac caggctataa acaaaaatca tcgatgactg aatcacatac attgatgtcg
    10381 tccactcaga cagcggaata ttgtactgtt ctataacgcc gccgatagtc gcgcgagtca
    10441 caaccgacgc cgcaataatg aaaaccgcca acaaaccaaa gagaaatttc ttccctttcg
    10501 ctgacttcag tttcgccata atcatgatgt aatccttgtg cagacaaatt tcttgttacc
    10561 taaacgtaac aataccatga catttcaccg ttgtctgctc tgaaaacctc acctcttagc
    10621 tggatttaca atcaacgcat tgattagtta gctaatgttg ctacttcctt ccctgactat
    10681 tgatgatctg aatgaattgt cgcggtaaaa tagccccgcc aggggtttcc ggcatgacga
    10741 caattatctg gaggagcctg ataaaacaat gatcgctgaa ctgtttacaa ataatgcgct
    10801 taatctggtc attattttcg gtagctgcgc agcattgatt ctgatgagct tttggtttcg
    10861 ccgcggaaat cgtaaaagaa aaggtttttt attccatgcg gtgcaatttt taatctacac
    10921 cataattatc agtgctgttg gtagcattat taattatgtt atagaaaact ataaactcaa
    10981 atttatcacc ccaggcgtta tcgattttat ctgtacgtcc ctgattgcgg ttattctgac
    11041 gattaagcta tttttgctga ttaatcagtt tgaaaaacag cagattaaaa aaggtcgcga
    11101 tatcaccagt gcgcggatta tgtcgcgcat tatcaaaatc accattattg tggtgcttgt
    11161 tctgctttat ggcgaacatt tcggcatgag cctttctggc ttgctgacct ttggtggtat
    11221 tggtggtctg gctgtcggta tggccggtaa agatattctg agtaatttct tttccgggat
    11281 catgctctat ttcgaccgtc ctttcagtat tggcgactgg atccgttcac cggacagaaa
    11341 tatcgaaggt acagtagcgg aaattggctg gcgaattacc aaaattacga cctttgataa
    11401 tcgtccattg tacgtaccga actcgctgtt ttcgtcgatc agcgtagaaa acccaggacg
    11461 aatgaccaac cgccgcatta ccacgaccat tggtttacgt tatgaggatg cggcaaaagt
    11521 gggcgttatt gtcgaagccg tacgtgagat gctgaaaaat cacccggcca tcgaccagcg
    11581 acaaacctta ctggtttatt tcaaccagtt tgccgactcg tcattgaata ttatggttta
    11641 ttgctttacc aaaaccacgg tatgggctga atggcttgct gcacagcaag acgtttattt
    11701 gaagattatc gatattgtgc agtcacacgg cgcggacttt gccttcccca gccagacgct
    11761 gtatatggat aacattacac cgcaggaaca ggggcgctaa atcagacatc actggcgtta
    11821 tccacaaggg taacgccagt tcccatcaca tcaatgcttc acaatataca tagtccggct
    11881 gtacgccaca tcttccgggt tattaatggg ataacctttc agccacggct tgattaatcg
    11941 tccattggta tattgataaa ttggtgcaat cggtgcttgc tccatgagga ttttttctgc
    12001 cgcgttgtaa tcggcattac gcgctttaac ggtattttcc gtcgatgcct gggccagaac
    12061 tttgtcgtat gccggattgt taaagcgtga aatatttcct gaatgcgttg acgttaataa
    12121 tgtcaggaaa gtggagggtt cattataatc ccccacccat gaggcgcgga taacatcaaa
    12181 attgccggtg ttacggctat cgatataggt tttccattcc tgattttgca atttaacatc
    12241 tacgccaagg ttctttttcc acatcgatgc tacagcaatc gcaatttttt gatggttttc
    12301 tgaagtgtta tacaaaagcg tcagcttcag cggtttttgc ggaccataac cagctgcgct
    12361 caacaaagtt tttgcctgcg cattcagttc ttcctgactc atttgttcaa acggcgaagg
    12421 ttccggcgta aatccggcgg taacatctgg tgtaaaatgc cacgctggct tttcgcccgt
    12481 ccctaatact ttttcggtca tcaggcggcg atctatcgtc atacttaatg ccagacgaac
    12541 gcgctgatct gccgtcggcc ctttttgcgt gttaaacgca taataatagg tcccgagctg
    12601 cggcggcgta taaacctgcc ccggaatatc cttcaacagc ttctgataca tatttttcgg
    12661 gaaggattcg gtgatatcaa tatcccccgc gaggtaacgc ttagtggctg cggattcctg
    12721 attaattggc aggaaggtca ctttttgcag taccgttttg gcgttatccc aataatgggt
    12781 attcggtacc acgaccagtt tttcattgac tacgcgttct ttaagaacat aagcgccatt
    12841 gccgatcaga tttccgggtt tcgtccactc tttaccgctt tctacgttgg ctttttgcac
    12901 cgggaagaag gcaaagttag cggttaaatt cacaaaccac ggcaacggtt tatcaagctg
    12961 aattttcaag gtatgggcat caactgcggt gacgccaagc tggtcaggcg tagctttacc
    13021 atcaataatt gcctgtgcgt tgttgattcc cgccagcgcg gcaaaccatg caaatggcga
    13081 tagcgttttc gggtccacca gacgttgcca gctgtagaca aaatcttgcg ccgttaccgg
    13141 tgtgccatcc gcccattttg cgttatcgcg cagggtaaaa gtccagatac ggttgtcatt
    13201 acttttccac tgagtcgcaa cgccggggac aatctcccct ttctcgttct gattcaccag
    13261 tccttcaaac agatcgcgaa tgacctgaat ctctggcagg cccacggctt tagcgggatc
    13321 cagcgacgca ggctcatctt taatgtggcg caccagctcc tgcttctctg ccagtactgt
    13381 gccgctcgga acttctgcag catacgaaag agaaatgctg ctgaccaaca gcgcacaaca
    13441 cgtgactgaa acagagtgct tcataagttt ccctctaacc gacattaaaa ttgatgcgta
    13501 attatttgtt tcgccttaaa gaaatgcaaa caacttccat caaaaaagtg acacaggaag
    13561 cataaaatgt tttatgtatc aatatattaa atcagattta gtgtaaataa tcgacgccaa
    13621 aatgcggcaa agcacataga acaccgaaca ctacgttcag gcttcgcacg ttcaaaccag
    13681 ttttcggtat caaaatcagc atctgtcgcc attgtacgaa gaataaaaaa taaccaaaac
    13741 gttgaaagga actgagaata cgttaagaga gaaattaaat taatgtatca gcaagtagtg
    13801 ctaaacagca cctttatact tcagcctatc tataaataat gagaatgccg agccagcatg
    13861 tcgacggtgt ggatagtaca ggtgataacc gggtaaatct ggtgtgaatt tatccaaaac
    13921 acgtatcagt tttttttctt taatagcccg ctcaacctga tcataaggta gatacgccaa
    13981 tccatgccca tcaattgcag catcaatgat caggtctatc gtattcagta aaagctgacc
    14041 ttccatgcga acacgaactt cacgtccacc gcgtattaat ctccagcgat ttgctgtacc
    14101 cgatgtggga agatacaaat taattgcctg atgatctatt aattgtgaca ctgacgttgg
    14161 aacacttcgg cgagaaaaat aatccggtga gccaacaata gccattggta tatctggccc
    14221 gattcgaatg gcgatcatat ctttatccat ctccccaccc agacggacgc ctgcatcaaa
    14281 acgttcagaa acgacatcgg tcaaaccata atcaatggtg agctgaatat caatttcagg
    14341 atgcgatttc aggaatgtgc gcattgctgg taacaatatc gtttttgctg catgttctac
    14401 agtagtaata cgtattgtcc cggatgggcg gttctgcaga tcgctcaggg atgccatggc
    14461 tgaatctatg tcatgcaaca tagggccaag aacagacaaa agatgctcgc ccgcttcagt
    14521 tggaacaacg ctgcgcgtgg ttcgcgtcag aagccgcaat cccaatcgtt cttctatacg
    14581 acgcactatc tggcttaaag ctgactgcgc catgctcagg cgggctgctg cacgagtgaa
    14641 gctacgctcc tctgcaacga cgacaaacgc catcagatca gcgatttctt cacgcttcat
    14701 tcagtatctc catgcaatct attaatcatt taaatgatag gtctaatcat gattttgggt
    14761 ctaatcaact taatgatcaa tatctatagt gatatcacca accgttaaaa cgaaccgaca
    14821 actccatgag gaacggaatg atgaataata aagtcagctt cactaacagc aataatccaa
    14881 ccatctcttt gtctgcagtg atctatttcc ctccaaaatt tgatgaaacc cgtcagtatc
    14941 aggcaattgt gttatctcac ccaggtgggg gcgttaaaga acaaacggcc ggaacctatg
    15001 ccaaaaaact ggcggaaaag ggatttgtca ctattgctta tgacgcatct tatcaaggtg
    15061 aaagtggcgg cgagccgcgt cagttagaaa acccatacat tcgtactgaa gacatcagtg
    15121 ccgtgattga ttacctgaca acgctttctt atgtcgacaa tacccgaatt ggtgcgatgg
    15181 gaatttgtgc cggtgcagga tatacagcca atgctgcaat tcaggatcgc cgcatcaagg
    15241 ccatcggtac cgttagtgcg gtcaatattg gttcgatgtt ccgtaatggt tgggaaaaca
    15301 atgtgaaatc tattgatgcc ctgccatacg ttgaagcggg ttcaaacgcc cgaaccagcg
    15361 atattagcag cggagaatat gccgttatgc cactggcacc aatgaaggag tccgatgcgc
    15421 cgaacgaaga actgcgtcag gcctgggagt attaccacac ccctcgcgcg cagtatccaa
    15481 cagcaccggg ttacgctact ttgcgcagcc ttaaccagat tattacctat gatgcttacc
    15541 atatggcgga agtgtacctg actcagccga tgcagattgt ggcgggcagc caggcaggga
    15601 gcaaatggat gagtgacgac ctgtacgatc gggcatcaag ccaggataaa cgctatcaca
    15661 ttgttgaagg cgcaaaccat atggatcttt atgacggcaa agcctatgtg gctgaagcaa
    15721 tttcagtatt agctccgttc tttgaggaaa ctctgtaatg aaacatattc aaatccgcaa
    15781 tagtgatatg gcctggcata ttgctgccaa tattcagttt ccaccaaatt ttgatgagtc
    15841 aaaacagtat ccggcgatta tcagcgtcca tccttttggc agttgtaagg agcagacgtt
    15901 tggcaatatc tatggcaaag cattggctga aaaaggctat ctcgttctcg cttatgacgc
    15961 cagtttccag ggagaatctg gtggggagcc tcgctggata gaagatccga cacagcgcgt
    16021 agaggatatt agccgtgtta tcgattatgc agtaactctg ccatatgtta atgctgaacg
    16081 tattggcgtg ctgggagtgt gtgggggtgg tggctatgcc attaatgccg cgctgacaga
    16141 aaaacgaatt aaagcagtag tgagtattac gggtgttaat atcgggcgtc tttttcggga
    16201 aggtttcagc aattacgatc cgattggcgc actgaatgca atggcgtcgc aacgaaccaa
    16261 agaggctcgt ggtggagaat tacagatcaa cgaattactt cccgccagcc tggatgcagc
    16321 aaaagcccat ggagtgaccg aacgcgatgt ttatgaagca actgactact ataaaacacc
    16381 acgcgggcaa caaccgggag gtgcgacaaa aatgttgttc tcacatgcac agaaaacgct
    16441 ggcctgggat gccttcgcat ttactgaagt gttactgact cagccggtga tggtagtagt
    16501 tggtgaaaaa gtcggtgcct tcggtgctta tcgtgatgga ctggaagtct atggccgggc
    16561 gatggtttcg caggatcgtc agttagtttc attacccgat ttctcacatt atgaactgta
    16621 tgacaaaccg gaagcagttc aggaggcgct ggaaaaagtt attccttttt tcaacaccca
    16681 tctgggataa gtttcatgag aaatattatg aaaaatgtac tgattcttgg tgctggcggt
    16741 cagatagccc gccatgtgat taatcaactg gcagataaac aaaccattaa acaaacttta
    16801 tttgcccgtc agccagcaaa aatccacaaa ccctatccaa ctaatagcaa gattattatg
    16861 ggagatgtac tgaatcatgc agcactgaag caggccatgc agggacagga cgttgtgtat
    16921 gccaacctta cgggggaaga tttagatatt caggcaaatt cagtgatcgc tgcgatgaaa
    16981 gcctgtgatg ttaaacgtct gatctttgtt ctttccctcg gtatttatga tgaagttcca
    17041 ggaaaatttg gcgaatggaa taatgcagtt attggtgagc ccctaaaatc attccgcagg
    17101 gctgcggatg caattgaggc ttctggactg gaatatacaa ttcttcgccc tgcctggctg
    17161 actgatgaag atattattga ttatgagctt acctcacgca atgaaccatt caaaggtacg
    17221 atcgtatcac gtaagagtgt cgcagcatta atcacagata tcatcgataa acctgagaaa
    17281 cacattggtg ataatatagg tatcaatcaa cccgtgactg atggtgacaa gccattattc
    17341 atgtaaatca attacatagt taccatcgta atgtactatg tgcagtacga tttattaaac
    17401 ttattaaggt gaactgatca atccgatgcg attaacatgt gtagaaccag taagaataaa
    17461 atacgccatg tatataacat tacgacttac atctaagtca gcataattct aatatctgca
    17521 catcaataaa aatgcaaaca acttccggca aaaaagtgat acaagatcga tgacatagcg
    17581 ttgaagaaaa ggatttgata atccgcaaaa tttgcacaac actgcctgta atacttcctg
    17641 aaccagagac gatcatgacc gtaaaccgcc cacgcgccga acgcggcgca tttccgcccg
    17701 gaacagaaca ttacggacgt tcattattgg gcgcgccgtt aatctggttt cctgcccctg
    17761 cagccagtcg cgaaagtggt ttgattctgg ctggcactca cggtgatgaa aactcttcgg
    17821 tcgtcacgct ctcctgtgct ctgcggactt tgacaccttc tttgcgccgc catcatgtgg
    17881 tgctgtgtgt gaatcctgac ggctgccagc tggggttacg ggccaatgct aatggtgtgg
    17941 atttaaacag aaactttccg gcggcgaact ggaaggaagg tgaaacggtt tatcgctgga
    18001 acagcgccgc tgaagaacgt gatgtcgtat tactgacagg tgacaaacct ggctcagaac
    18061 ctgaaaccca ggcgctgtgc cagcttatac accgcattca acctgcctgg gtggtctcat
    18121 tccacgatcc actggcctgt attgaagatc ccagacacag cgaattaggc gaatggctgg
    18181 cccaggcgtt tgaattgcct ctggtaacca gcgtcggtta tgaaacgcct ggctctttcg
    18241 gcagttggtg tgcagatctc aacttgcatt gcatcaccgc agagtttccg cccatctcct
    18301 ccgacgaagc cagcgaaaaa tatctctttg cgatggcaaa cttgctgcgc tggcatccta
    18361 aagatgcaat tcgcccgtcg tgaactgaag cgccggttcc acatctaccg ccagccaggt
    18421 cggtccgtca agatcggcga aactgacctg cggcaccagc ggtaaagcgg cgctaatggc
    18481 acgagaggta cacaacatgc agcccagcat cagactgaaa ccttgtgcac gcgcttcagt
    18541 cgccagcgcc agcgcttccg tcagaccacc ggttttatcg agcttaatgt taaccatctc
    18601 atagcgccct ttcagcgcct tcagattgct acgtgtatga caactctcat cagcacaaat
    18661 cggcaacgga tgaataaaat tctccagcgc cgcatcgtcc tgcgcaggaa ggggttgttc
    18721 aagcatcgca acgcctaaat ccgccaatag ctggcaacgc gccgccaacc cttctgcacg
    18781 ccaggattca tttgcatcaa cgatcagcgt cgcatcgggc acagctgtgc gaattgccac
    18841 catccgctca ctaataagat ggttatccag cttcactttc agtaatttcg cgcctgcctg
    18901 ccagagtgtt gatgcactat tggccatctg atcaggcgta ccgatgacta ccgtctgtgc
    18961 agtgataact gtctcgggaa gcgttatgcc gatcaaatca gccagcgatt gctgctgttt
    19021 tcgcgcggcc agatcccaca atgcacaatc cagcgcatta cgtgctgcgc cggcagggag
    19081 aattttttgc aactcctccc gtgtcagccc tttctctaat tgcgacacga cgctcataat
    19141 ttgcgccatt accgaggcat cactttctcc ataacgcgga tacggcgtgc attcgccggt
    19201 gcctttaata ccctcttctt ccagttcaac caccaccacg cgcgcttcac tgcgacttcc
    19261 ccgggcaatc acaaacgggg tatgtaaggg ccaggcttcc tcaaatacct taacggttct
    19321 catctttact ccttgttgtc gcggtggtct gcggattaag cgcaaatagc gtttgctgtg
    19381 ttattgacag ttagcataaa ctaggtgtga cgttaactat atgtaaacag gaaagataat
    19441 catgtcacaa accgttcatt tccagggcaa cccggttaca gtcgccaatt ccatcccgca
    19501 ggcgggtagc aaagcgcaga cttttactct cgtagcaaaa gatctgtctg acgtcaccct
    19561 cggtcagttt gcgggtaaac gcaaagtgct gaacattttc ccgagtattg ataccggagt
    19621 ttgcgccgca tcagtacgta agtttaacca actggcaacc gagatcgaca acaccgttgt
    19681 gctgtgtatt tctgccgatc tgccgttcgc ccagtctcgt ttctgcggcg cagaaggtct
    19741 gaacaacgtt atcaccctct ccactttccg taacgctgaa ttcctgcaag cctacggtgt
    19801 ggcaattgct gatggcccac tgaaaggtct ggcagcgcgt gccgttgtgg ttattgacga
    19861 aaatgacaat gtgattttca gccagctggt ggatgaaatc accaccgagc cggattacga
    19921 agcagctctg gctgtactga aagcataatt taatatgcct gatggtgttg caccatcagg
    19981 catattcgcg cttactcttc gttcttcttc tgactcagac catattcccg caacttattg
    20041 gcaatcgcgg tatgtgaaac gccgagacgt tttgccagtt tgcgcgtgct gggataattg
    20101 cgataaagct gggttaatac cgagcgttca aaacggctgg tgatttcgtc cagcgaacct
    20161 tccatcgcat cttcgcccac ggctaccgtt gcggcgtcat aatccggcaa caaaatatcc
    20221 tgtggacgca gctcataacc gtccagttgt gtcagtgcgc gatagatagc gttctttaac
    20281 tgccgcacat ttcccggcca cgcataacgc gtaagtacag tattcaggtc agcggccagt
    20341 ttcggacgcg gcacgccctg ctcgtcggca aagcgggcga cgaacagctc agttaacggc
    20401 atgatgtcct gcggacagtc acgtagcggc ggcagattga gcgtcaacac gttcagacga
    20461 taatagagat cttcacggaa catgcctttt tgcaccagtt cgaccagatt cttctgcgta
    20521 gcgcaaatca cccgcacatc gacatgcacc tcatggtctt cgccaacccg acggaaagtg
    20581 ccatcattaa ggaaacgcag taatttcgcc tgcatccgtg gtgacatttc ccctatttca
    20641 tccaacagca ccgaaccacc gttcgcctgc tcaaagaatc ctttcttccc ttccggagca
    20701 tgaccaaaca gttcactctc gaccgcatct tccggtatag acgcacagtt cagcgccagg
    20761 taaggtttgc ccgctctggg gcttgcctga tggcaggcgt aggcaaagag atctttacct
    20821 gtacctgtgt cacccgtaat cagcagcggc gcgcttagca tcgccagttt ctgcgcctgt
    20881 tcgacaacat gcttcatttt cgggctgacg gcgacaattt gactgaaggc gctgacgtcc
    20941 tgggcggcga cattttgcaa ctggcggccc atacgaatcg ttgatcgcaa catcaccacc
    21001 gcaccggtca ggacgtgttg atcattttca tcctgaagat aaacaggcgt aatctccatc
    21061 aggaaattct gcccattaat aacgacatgc tcgttatggg aatcttgcgg ttcgctttcc
    21121 agccaacgta aaaaattaaa gccgttaatc aattgtgcgg cggtatggtt gcgcaggcga
    21181 tccaattttt gcgcaaaaag ctgacagctc gccgggttcg ccatatccac tttgcttttc
    21241 atatcgacag agagcacagg ttcgggcaac gcctccagta acgcgctcaa cgccagatgc
    21301 tcacgttcgg aaggcatcca cgggacagta cgcacatcgg taacacctgc aatacggcgt
    21361 atttcggcca tcagactgct gaaactctca aactccagtt cagcaaaatt gaggtagatt
    21421 cgcccaatgg gatcaatctc aataccgcgt aaatcaatgc ctcttagcac gagtagatcg
    21481 agtaattcgc gggtcagacc gagtcggtct tcacaaaaga cttccagacg catgggaacc
    21541 ttcacctgaa aaaagaacaa ttaatatgat gatatgacac tatttggata gcaggaaggt
    21601 ttctgtcaac aatcattgac acaaagacgg aaaaacgggc acttcggcgt aaagatatcc
    21661 ggtcagcacc cgatattgca ttatttttcg ctgggcgttt tgcctttttg cagcgtttct
    21721 ttcacctgac cgataagctg acgacggaaa tccccgaggc gaggtttgtc atcgtcaatc
    21781 cacggcagcg ggcggcaaag ctccatagct ttaatcccga gtcgtgccgt cagaagtcca
    21841 gcaccaattc cctgagctgc gcgggtagac aaacgagcag cgagatcttg cgacatccag
    21901 tccatcccca cttcgcgcac cagttcgctg gctccggcaa aagcgatatt cagcaatacc
    21961 agcttaaaca gacgcaaacg gctgtaatac cccagttcaa tgccatacag cgtggcgatg
    22021 cgattaatta aacgcagatt gcgccaggcg ataaacgcca tatcgaccaa cgccagcggg
    22081 ctgaccgcaa tcatcaacgt tgattccgcc gccgaacggc tgatttcgcg ccgcgcctgg
    22141 gcatctaaaa ctggctggac caaatgcgca tacaaactga ccacttcacg gtcgttttgc
    22201 gtttcatgga ttgaggcata ccagcgttgc agcgctggat gagactgatc aatacccgcc
    22261 tgctgcgcca gtttttcgca aaatgcgcgg cctttgcccg tgccgtggct gtgcaacaaa
    22321 tcgcgcgctt cgtcgcgttc atgggcgcgc tgtcgcaagc gccataagcg ccgccactct
    22381 gttaccacag tacctacgcc agcgccgata atcaatgccc cagcggcaca tccacccagc
    22441 gccacccagt cctgagtttg ccaggcattc attgtccact gtacaccctg cccgacaacg
    22501 cttgcgccaa acagagccag cccgcccatc accattttgc gccacaggct gcgtttcgga
    22561 cgtaacgctg catccattac cgcttcaact tgcccctctt cttcaggcgc ttcatcgagc
    22621 gtggccgggg caaaattttg cgcctgattt tcgtcaaagg tctgctgcgc cctgaatttt
    22681 ggattctgat cgacctccag cggaccgtcg aaatcaatac gtggttttaa cggttcggtc
    22741 atcgcaattt atctcctatt aaaaattcaa gcgcggcatc aagacgaata tgcggcagcg
    22801 gtttgtcgac atccatcacc tgcgggcgaa acgcttcaaa ctggaaccct tgcttgtccc
    22861 agaacgcctg accgggcaaa cgcgcaggaa cttcaccggg ataaacggtg agtggcgcac
    22921 catcgctgag ccggttaccg cgcaatgccg gaattttctc gccgttgacg tcaataatgc
    22981 cgctggtggt cgcctgcacc gaggccagcc ccaggcaatc catactaatc ccttcaaacg
    23041 ccgcattttg ccaggcatcc tgaatcagtt gttgcagtaa tgaaaccata ttggcgtgct
    23101 gatcgatggt cacatggtcc gctttagtgg cagcaaacaa tagcttatcg ataaccggcg
    23161 aaaacaaacg ccggaacaag gtacgctgcc cgtagtggaa actttgcatc agctgcgtca
    23221 gtgccagacg catatcatta aatgcctgtg gcccactgtt gagaggttgc aggcaatcaa
    23281 ccagcacaat ctggcggtca aagcgcagaa aatgattctt atagaacccc ttcaccacct
    23341 tctcgcagta ataattaaac cgctcgcgca gcattccggc attggtgtgc ttatcggcct
    23401 gcgccagttt ggactcgccc caggcatcga catccggcca cgggaagaat tgcagcgcgg
    23461 gcgcacctgc catatctccc ggcaagacaa agcgcccagg ctgaataaag tgcagcccct
    23521 gctgtttaca gtggtggaga taatcggtcc acgcggcggc aatgtctgcc agccggtttt
    23581 cgtcggcagg cgctagcggg tccagccctt cgcacatcat tcgccatttg accgaccatt
    23641 cgccgcgctg accattgagt aagcccgtca tctggcgtga ccagcttaaa tagtcctgcg
    23701 ccagcatcgg caggtcgagc aaccattcgc cgggataatc cacaatctcc agatacagcg
    23761 tggaggtgtc cttgaagtgg cgtagcagcg aatcgttcga tttaaaacgt agcgccaggc
    23821 ggatttcact gacgccgcgc gttggcgtcg gccaggcggg aggatcgcca tataactgcg
    23881 ccagtccttc atcatatgtg aagcgcggaa tgccaaagtc acgctgagga atgcgtttta
    23941 cgcccagcag gcgctcttca cgcaccgcgc ttaacagcgg caagcgtgct ccggcgtgaa
    24001 tattgagcaa ctgattgacc atcgcagtga taaacgctgt tttgccgctg cggcttagtc
    24061 cggttacggc gaggcgcaga tgtctgtcga caccccgatt caccagcgca ttaagttcat
    24121 ttttaagtcg cttcattgct gtcctgtgct gctctgaaga ccagaaaaaa atcattcgta
    24181 aggatatgaa tgtgggggcc aggccattga ttatcaactg cccgtgttgt catcgacggg
    24241 cgaactccat aagatgtgca gcattcacgt tcggacggag ttcctgacct atgtccccta
    24301 ctatttatga tattgccagg gttgcaggcg tatcaaaatc caccgtatca cgcgtgctga
    24361 ataagcaaac taatatctcc ccggaagcgc gcgaaaaagt gttacgggcc attgaagaat
    24421 tacagtatca gccaaacaag ctggcccgcg cgctgacctc ttcgggtttt gatgccatta
    24481 tggtgatttc tacccgttcg accaaaacta cggcgggtaa tccgtttttc tctgaagttt
    24541 tgcatgccat caccgccaaa gctgaagagg aaggtttcga cgtgatattg cagacgtcgc
    24601 acaatccggc agaagactta caaaaatgtg aaagcaaaat taagcagaaa atgattaaag
    24661 gcattattat gctaagttcg ccagcggatg agtcattttt tgcccaactt gataaatacg
    24721 atattcctgt cgtggtgatt ggcaaagttg aaggtcaata tgcccatgtt tattctgtcg
    24781 ataccgataa ttttggcgac agcattgcgt tgaccgatgc attaattgaa agtgggcatc
    24841 aaaatattgc ctgcctgcat gctccgcttg atgtccatgt ttcagtggat cgggtaaatg
    24901 gttataagca gagcctggct gcgcataata ttgcagtgcg tgatgaatgg attgttgacg
    24961 gcggttatac ccatgaaaca gcactgcaag ccgcgcggga attattaagc cagtcaccgt
    25021 tgcctgaggc agtgtttgct actgacagcc tgaaattaat gagtatttat cgtgcggcag
    25081 cagagaaaaa tattgctatt ccgcagcagt tagcggtggt gggttatagt aatgaaacgc
    25141 tgtcgtttat tttaacgcct gcgccaggcg gtattgatat cccaacacag gagttaggtc
    25201 aacaaagctg tgagttatta ttccgcttaa tttctggaaa accgtcacca cagaatatta
    25261 ccgttgccac gcatatgacg ctgaaataaa taccagctac cttgcctgaa tgccggatgc
    25321 gatgctagcg catcttatcc ggcctacatt tatagttcgg ataaagtact cgtgccgcat
    25381 ccggcaattt agcccattat cagaacgagt aatttacgcc gacacctgca taatggaatt
    25441 tatcgctgtc gccttcgtcg tgatcctgcc actcaaacgc gtattccagc gaaacggaaa
    25501 gcccgttctg gaaatcataa ccgtaaaata aacctacacg gttaaaatca tggccttcac
    25561 gttcaatatc atcctgccag tcccagttac tccagcgatc cagcccaatg cgcgtatacg
    25621 gcgtcaccga gtggttgccg agcgtcagcg gcaaataggc gcgaatttct tgcgtagaaa
    25681 actcaccgtt attgcggctg tcgtccatat taaagccgcg ctcgagataa tagttcactc
    25741 gcaaggcaac cgtttcgttg aaggtatatt gcaggcctgt ttccgtttcg actcgggtat
    25801 cagcgtaacc ggtcgtgttc agatcgttgg caaatttata catcgacaac caaccgttga
    25861 aacgtaaatc gtcagtcagt ttcacatccc agtctggcgc gattttccag cgctgcatat
    25921 tcgcagtgtc tttacccggt tcatcaacgt agtgataacc ataattacgg aaaccgccgg
    25981 tcaggccgaa actgaaatca tcgttttcga ggaactgata atgcacctcc agctccgggc
    26041 gatcaaacca cgttccacgt ttacccgcgc tataatctac cggcccttcc tgataatagg
    26101 ccagagcaat tctccacggc ccgttggcgg cattaaaata gactgaaggc tccgccagcc
    26161 catccatatc ttcgccataa ccctcgacgt tttctatttc gtacatcgcg ccgatattaa
    26221 agtgccagtc gttcctttcc tcggcctgtg cgcaggccat tcccgcacac atcaccagtg
    26281 cggtacaggg taataacttt ttcattattg ttccttgtat tattctgggg gaatgccttc
    26341 cccctgtatt tagcgaattg ctatttccgt ttctgcatca aagaaatgac atttcgtcat
    26401 atcaaaatga atagtgatat tttctcctgc atgataatca tttaacgcac cagcacggac
    26461 cactaactcg tgccccccaa ccgtggtgta gagcataaat tccgcaccgg ttaattctgc
    26521 cacgctaatt ttggcggaaa tgttattttc ctcttgcgcg tccggatgaa tatcttccgg
    26581 acgtattccc atcacgatgg gcttatgcaa acttccctgt gttttcagaa ccgctaattt
    26641 ctcttcggga atggttaatt taagcgtttc cgtaacgaat ttatcgccat cgatcgtgcc
    26701 acgaataaaa ttcatcgccg gtgatccaat aaatccggca acaaacatat tcgctggttg
    26761 gttataaacc gttttcggcg caccaacttg ctgaacaatc ccatccttca taatcacaat
    26821 ccgcgtcgcc atggtcatcg cttcggtctg atcgtgggtc acgtagatca tggtggtatt
    26881 cagcttctgg tgtagcttgc tgatctctgc gcgcatttgc acgcgcagct tggcatcaag
    26941 gttagagagc ggttcatcca ttaaaaacac gcccgcttcg cgcacaatcg cccgcccgag
    27001 cgccacacgc tggcgttgcc cgccggaaag cgcgcccggc ttgcgtttca ggtactcacg
    27061 caggccgaga atttgcgccg cccagttcac ccgctcatca atcacctctt tggcgatttt
    27121 ttgcatcttc aggccaaacg ccatgttgtc gtaaaccgtc atatgcggat acaacgcgta
    27181 gttctggaac accatcgcta tattgcgtgc tttggctgga acgtcattca ttcgtttgcc
    27241 gtcgatcaac agatcgccgc cgctgatctc ctcaagcccg gcaatcatgc gcagggtggt
    27301 cgacttaccg cagcccgacg ggccgacaaa cacgatgaac tctttatcgg caatttccag
    27361 gttgaagtcc ttcaccacat gcacctggtt atcgtagatt ttttgaatat gttgtaacga
    27421 aagctgagcc atgttgattc ctttgctata cgttttgcca gaaggccgat aaccgcgacc
    27481 aggtcagaga gtccgttgaa ggcaacagta attgcgcccc ggttaagccc gcgccgatcc
    27541 ccaccgagcg cataccgctg gcgttaatgg cgtcaatgcc cgcctgcgca tcttcaatgc
    27601 cgatacatgc ctgcggcggc acgcccagcc cggcacaggc ggcgagaaag atttccgggt
    27661 ccggtttcga gtttttaagt tgggaagcat ccgcgcagaa ggtgaaaaac tcgcgcagct
    27721 ccagcgccgc taaaatcgtc ggcgcattca gggagacaga agccagccca accgggatct
    27781 gctgtgcacg gagatctgcc agcaaagagc gaatgccggg tagaacagcg ctgaccgtca
    27841 actcgcgcag tgagtggaca tagagcagat ttttgcgata cgccagttgc gccctctcct
    27901 gcgagttaaa gtcgccctct ttgcccccgt gttgcagaat gcgccgcaga gactcatcgc
    27961 ggctgatccc ttttagggat tcgttaaact gcgcatcaat gctgatgcca atttcagcgg
    28021 caatctgctg ccacgcctgg aaatgcagat gcgcggtatc ggtgattaca ccatccagat
    28081 cgaaaattac cccttgcagt ttcattcatc ctcctgatgt gtggtagcgg tcccattgaa
    28141 gggcaaaata aaatcaccca aacagaaaac agattcttca gccacggata taagctgacc
    28201 gttcaaacgc agtgaaacgg gcgcagaagt tcgaatcgca atacgctgcg cgtcaagagt
    28261 gacctgtaat tcgcagccct gccagaacag agggaaagac aactgttgcc actgctcagg
    28321 taacgccgga ttgagatgca attcaccgtc acgcacgctc accccggcaa aaccctgaat
    28381 cgcccccagc cagatagcgc cagttgcggc agcatggata ccatcatcac aactatgcgg
    28441 atcagcacca agatcgattt cagtcccctc gcgccagaac tgataacttt gggtcagcag
    28501 gccacagcgt gcggcaacaa tgccgtggat tgctttactt aatgacgagt cgtgaatagt
    28561 gcgcggttca taaaattgca gattggcaag acacgatgcc gctgagaact gctccggcag
    28621 catgtaattg agcatcacca catcagcttg tttgaggatt tgcatctcgt tcacttctgc
    28681 gcgtgaataa tccagcagaa tggtttgctt ccccgccgcc gctttgtatt tcgccagatt
    28741 aatcgccggc ttagccataa acgaatcatc ctgcggcaaa acgccgtcgg gctgaatttc
    28801 tggcagccgc agttctttaa ggaacatttc ggcgcgatgg ataaacgcat cgtcgctaca
    28861 gccaaactgg cgggcaatac tcagcgcctg ctggacgttg taatacgcca tatagctggt
    28921 gaaggcgtta ttattgacat gttcggtata ttcgtcaggc ccaataacat catgaatttc
    28981 cagacggtcg ttaaccctca ccgcgcggct aatccagaac tttgcagttt ccagaagtag
    29041 cgccatgcct tcatgcgcaa tgaaactttc atcccccgtg gtctgccagt attgaataac
    29101 cgcccaggcg atatcggcca ccagatgatg ttccgcctgc gccgaggcca ctttttgccg
    29161 cagcccggtg cgaatgttaa tggcggcaaa ttccggcgtc tcttcttcgc cgctgcgcgc
    29221 gctttcccac ggaaatagcg cgccctgcca gccgttgcgt cgcgctttct cctgcgcgcc
    29281 tggcaagttg tgccagcgat aacgcagtaa acttcgggca accgtcggat cgctaaacag
    29341 atgaaacggt aacaaaaata cttctgtatc ccagaaaacg tggcctttgt agccttcgcc
    29401 ggttaagcct tttgccgcaa tgctgctgcg ctcgtcgtgg gcaggcgtca tgatgcgcag
    29461 atgataaagc gcataatcta acgcttgctg atcgtgcgct tcgccgccat ttaccgtgat
    29521 acgacgtttc tgccaccatt gacgccagtt ttctgttgat actgcaagaa gttggtcgta
    29581 actctgctgc gcgcacattt caagctggcg aagcgacgcg ctgccccact cgtctaaaac
    29641 ggcttgcctg tcatcccgcc agtcgatcca caccagtttt tgcaacgtca ctgtctcgcc
    29701 tgcatgaagc tgcgcactgg tatgttgctg caaacggcgc tctttggcgg tatagcattg
    29761 ctgcacatca ccgctcacca tacagcaaca gctgatggcc acatcactgc gtccatcctg
    29821 ggtggtgtag atcccctgca tcagatgctg accaaacacc cgcacctggg tttcgtcgag
    29881 atgttggcga ccgtggttgg tttgcgtggc gtcgatacct gttgaaatca gcactgacgc
    29941 gtcggcgtcc agtggcgtaa tagtgatttc cagcacaatg agcggcagtt ggtctgccga
    30001 aacaaagcga cggctggcga tggtgtaacc tgtgccgttg ctggtacgcc agacaacgct
    30061 acggcgtaat tctccactgg caaagtcaag ttcacgctgc caggcttcgc gggataacga
    30121 gaaaacctca ccatttatgg caatctccat ccccaagata tcaggcaggt tcaccagttc
    30181 gttgatttca ccttttcccg cccgatgata cagccccgcc agatacatcc ctcgcgtctg
    30241 gcgcgtgtaa tcttcttcat ggctggcgcg aagcccaaga tagccgttcc cctgcgccat
    30301 cagcgatgca tacttgttca gggtatgctg gctgaaatgg ggttctgata acgttactgg
    30361 cctggtcata attccacacg cgtccctgtt tctgcggatt gatacaacgc cgccaccagt
    30421 tgctggatga tgtacccctg ctcggcgtcg gctatcatca cgggcttgcc ctgtacgtga
    30481 ttgataaagg cttccatgct gcgcaaatgg cggttgtcgt ctgccagttc ccgttgcatc
    30541 agcgtcatta attcaccgtt gttatcggtg tagatatgtg ctggaaacag cgtcgcaccg
    30601 gctttatcac cacaaaagct gacgttcata atcgactgtt cgcggatgtt gagtgcaaat
    30661 gacgtttcca gccacagaat gccgccgtta tgaaattcaa tggtgccaaa taacgaatct
    30721 tcgacgctgt aagttgccgg atcccactcg ccaaattgac cgcagctctt ttgcgtgccg
    30781 atcttttgaa agctatgcgc attcacgctt ttcaccgccg gaaagcccag cacatacatc
    30841 gccgcatcca gcatatgaat gccgatgtcg ataagcgggc caccaccctg cagttcttta
    30901 ttggtaaaga caccccagcc gggaacgccg cagcgacgca gggcgcgggc ggtggtaacg
    30961 taaatctctc ccaaaacgcc gttggtcacc tgttcacgca gctgttgcgt atcgagcgca
    31021 aaacggtggt gaaagtcgta ggccagcacc ttgcccagtt tgcgcgcggt atcgcacatt
    31081 tcccgcgcct gttctggcgt catggcgggc ggtttttcgc acatcacatg gcagcccgct
    31141 tccagtgcca tcagggtatg ttcgtaatga aaacggttag gtgagcagac gctaaccaca
    31201 tcaggtttca ccgccagcag catggcctgc ggatcgtccc acacggatgc attaccgtat
    31261 ttatctgcca gcgcctgcgc ctgggaaagg cggctgtcac agacagccac cagttccaga
    31321 tcgttgcggg tgcagtagta cgaagcatga accttatccg ccacctggcc tgcgcctatt
    31381 atcgcgaccc gcagcggaga gcttgtcatt gcacttttca caaagacctc ttagcaggta
    31441 cgcaaccagg ccaacgaatc acggtacgcc tgggcaggat cttccgcccg gatacgccct
    31501 tcgtacacta catagccctg atagttatcc gcgcgcagct gttcaaacag cgcgtggaaa
    31561 tccagggtgc cgctgcccgg ctggtagcga tggttatccg caatatgcac atgaccgagc
    31621 aggtcgcggt tgtcatgcag cgcctgcgcc aggttatctt cttcgatatt catgtgatag
    31681 aaatcgccga taatctgtac atgtttaaga tcgttttcga cgatgtaacg gcgggcatcg
    31741 gcgagggtgt tgatcatatg atcctgatag cggtttaacg gttcgagata caccacggtt
    31801 ccggtacgcg cggcgacctg ttccagtacg tgcagggaat cactcaccat tttgcggtcg
    31861 ccgtccaggc tacgcggcga ggtcatcggc ggtaagcgga aggtaaacat gccccacgct
    31921 gccggaacga cgataccttt accccccact tctgccagcg cttcgagaat gcgttcgatc
    31981 tgctttaagc cattaagacg acgctcttcg ataaagtcgc caatccaacc gtcatagcca
    32041 ccgcaggcgg tggtcaccgg taaaccggtt tctttgattg ctgctttgac ttcttcgaga
    32101 ttgttgacca gcagtttgcc gtcaatctca aaaccatcga agcccatctc tttgatataa
    32161 cgaaatttct ccagaatgtt ttccggaaag aatgcctgat tctgtgtgcc tattttcatt
    32221 ttttcattcc tttgaattaa aacgtaacgc ccattttgat gctctgttcc ggatgctgat
    32281 cgacatactg catgtagctt tccgggctct tggcaaaggt cactaccggg tcgattaaat
    32341 cttcgcaatt gagataaccg ttcatcagca gttcccagca ggtttcttca atacgcttac
    32401 ggctccagcg cggataatcc gggttcggtt cgctgcacgc acgggagaaa acaattttgg
    32461 cgttattgaa atgcgcttcg cgtccgaggt taaaaccttc agcaaatggt ttggcaaacg
    32521 cgacataaga gatggtgccg ccataagcaa ggccacgcag cgccgattgc agcgcgtcgg
    32581 cgtaaccgct ggtttcgata atcacatcgg caccctgctt gccggtcagc gttttgatct
    32641 cttttcctac gtcagtgcca atggggttaa ggcagaaatc agcaccgtgg cgacgggcaa
    32701 tatcacagcg ataggcgata ggatcgacgc caatcaccac cgacgcgcca gcgcgtttcg
    32761 ccagttggat ggctatttga ccaatcgcgc caagccctac caccaccaca aaatcaccca
    32821 cgcgcacgtt ggcatcgcgc acgccgctca tggcaaactg cgccgggtcg taacaaacgg
    32881 cgtttttcca cgagctgcct tccggcattt tgcgcagctt gtagttattc accgcgttaa
    32941 tgatgaccgt ctcggagagc gggccgtagc cgcatacgct gtcgcccacc gcgtagtcgg
    33001 taacgtcgct gccgcactcg ataatgtcgc caaccaccat gttgccaagc tggaatttgc
    33061 caaactcaat gccgcgcggc gcatctgccg gacgcggagt gaacatctgc cattcgccgt
    33121 taaagtcttc atcgataaac gggctggcgg cgcggaagtc gaccacttcc gttccgtgtt
    33181 tcggtgcgcc gaaacgcacg cggattttca cttcattggc taaaatcgcc cgatcttcat
    33241 actcaaccag cgcagcaaca cgcggtgctg tggctactaa ctttttcatt taaaactcct
    33301 tgtaaagaaa ctggccgctt tagcccttca cgccaccggc ggtcaaacca ctcttaataa
    33361 aacgttccga aagcgcatac ataatcacca ccggcaatgc ggtcaccagt gaggccgcca
    33421 tcatccgtcc ccagatgtaa tctggcgtac tgaacagcgc gttcaggccc accggtaagg
    33481 tgaaattgct ggcgctggag aggaaaatcg acgcaaacag atagtcgttc cacgccacca
    33541 taaagcagta gacgaacacg gatatcagcc cggacatcgc caacggcacg gtgatgcgga
    33601 agataatttg cagacggttg aggccatcca tcatcgccgc ttcttcgatc tcatcgggga
    33661 tggtgtcgaa gtagcttttc agcataaaca cggcggtggg cagagtttgc gtcaccatgg
    33721 tgatgatcag cgccatctcg gtgtcgtaaa tgcccagcgc ggtgatgatt ttgaacagcg
    33781 gcaccaccag caaaatgcca gagaacatat acaccgtgta aaagctggca ttgatggtca
    33841 tccgaccttt aaagcgcagg cgggaaagcg cgtaagcccc aagaatgccg aggaataccg
    33901 ccaccacgga tgaaaccacc gacaccacca gactgttacg gaagtagtcg acaaacggga
    33961 aaatcatcgg gttaaaaatg tcgacgtaat gctccagcgt ccactgctgc ggcagcagcg
    34021 taggatgcag tgagatcgcc tctttcgcgc ccttgaacga ggtcatcagc atcacaaaaa
    34081 atggaaacag cgtgatgatg agaaacagcg ccagcccgca gtaaaaaccg atgcgactga
    34141 gtgtgcgttt atttgttgcc attgaggttc acccgtttcc tggtcagcag aatgacagcg
    34201 aaaataatga tgaagagcac caccgagatc gccgccgctt tccctaagtc attaaaggca
    34261 aaggcggttt tgtagagata tacaccgaga atatcgacct tggtcgtcag caaataaaca
    34321 tccgcgaaca tgtagaacat ccagatggtg cgcagggtca ccaccgtcgc caggaccggc
    34381 ataatcgcgg gcagcgtgac gatacgaaac cgttgccagg cattagcgcc atccatctct
    34441 gcagcttcgt acagcgattt gtcgatggtc tgcaaaatcg ccagaaacga gataaaggca
    34501 tacgggaagt agcgccagat ggcgaacagc accaccagca caaagctact gcccggatta
    34561 tcgaaccaca gcggtgcctg ctcatagaga tgcagaagat cgacgccgag gtagttcaca
    34621 atgccgtagc cgttgttgaa catgtatttc caggcaaaca ccagcgaaat ggacggcgtt
    34681 acgtaggaga ggatcaccag cgaacgcgcg gttttgcgca gatggaactc gcggttgaaa
    34741 aacatcgcca ctgccagccc gagcacggtg ctgcccgcca ccaccagcgc ggtataccag
    34801 accgtcatcc acagcgagtg ccagaagccg ggatcggaga ggatacgcac atagttactc
    34861 accccaacaa aggttgactc gatgttggga ttgagcggca gacgtaaaaa actgatttcg
    34921 atattcgaca ccattggcca ggccaccaga ccacccagca gtaataagct gggcgcgaga
    34981 agcagcagcg caaagggcat atcggaacga cctgaaaaaa gcctgttcat tgcggattcc
    35041 tgtcctgggt ttagtgctgt tcgatcagtt catccagctt tttctggctc gcttgcagcg
    35101 tgccagggag atcggcttta cccacggtga cgttatgcac catggagctc accactccag
    35161 accccgtcac atcacccatg cgggtaaagt ttttatcccc tactgcacca aagacctgaa
    35221 tatttggcag ttcaccgatt agctgattcg gtagttcacc cagcgcctta ataacgtcgt
    35281 tgtctttcca ggtggcggta gtgacaaccg ctttattcac cggcagcgca gcacctggcg
    35341 acatcatcac ccagtcggca atgttgtctg cctgctccat aaaggtgaca aatttttctg
    35401 ctgcttccgt ctcttcggtc ttttgcccgg cggtaatggt cagcgaggtc aacatgccgt
    35461 agaccgcaga gtttttctcg gttggcacca cgaaaccgac gtttttcggg tcgccttctt
    35521 taatcacagc cagaaggata taggtggagt aaatcgccat cggcgcggtg ccgttcataa
    35581 aggcgtcttt cacttccatg atgtcgttag aacccggcat agtgttggca gcaaggtcgc
    35641 ggtaataggt cagtgcctgc atcatctctg gtgtatcaag ggtgattttg ccttcggcgt
    35701 taaagacgtt agcctggttg gataacgcaa actgggagaa ggattgttcc gtcaacacgc
    35761 tttctgctgt aggcagcgca atgccgtatt ttttattcgc cgggtcattc agtttctgtg
    35821 caacgtccag cagctgttgc cagtttttcg gctcctcaag ccccgctttt gccagcacat
    35881 ctttgcgata ccagataccg ccaatccagg cgctgacagg aacaccggtc catgcgctac
    35941 catcttcggt acgcacaata cgcagtacgc catcgtaaaa cgcgccttca ccaacgttac
    36001 tgatgactgt ggcaaccgct ttgcgatcga taagctgctc tttgtccatc actttggcgt
    36061 agtcatggct ggtttcgatc acttccggca gcgaaccgct acgtgaaaga gtaatgactt
    36121 tagtgttata ggcatcttct tccacgggca cttgcttaac ggtgatgcca gggttttctt
    36181 tttcaaaacg ggcaatcaat ttactgataa cggcctggcg ctcctgctcc accgaagaat
    36241 gcataaattc gatggataca ttcgttttat tttcttcttt acagcctgaa atcagggcgc
    36301 aggaaaccag tgctgataac agcacgattt ttgatttaat catgagatgt tcctttttat
    36361 ttaatccaca taacctgcca ggggcgcagc gttatttcat tacccaatat atcaacttca
    36421 ctaatcagat cccgaccatg cagattagta atatttacat gctgaatatt tttactgaca
    36481 ttaaacaatc cagtcagaca attaccatca gcgttacttc tttgaatacg cattacggat
    36541 gaattaatcg tatcgatggt aaaattatta tccggataaa actcgttatg gctgcgacga
    36601 agtgtaatta aacgcgacaa ctcatgatat accgcatgcc ttaatgtagc ttcatcgttc
    36661 agttctcggg ttatctcttt actgtgatat tttttacggt taatcgcacg gttatatccg
    36721 agtttttcga cacctgcgta atcattacgc gagccaagaa tactttgaat atatatcgct
    36781 ggcacaccgg ggaaacttaa caaaatcgca tgggcgagaa taaacctggc gcaacgttct
    36841 tcatcgctac tctcacggcg gcttaacgca tccatatagg tcacatttat ttcatacgga
    36901 ctgcgtgtac cgtcgggatt atttttccag tttactaatg caccttcctg ctgtaacgcc
    36961 tcgaccagct ctaatatttc gctttcaggc aacaagcccc gtagcgggtt tagcccgatg
    37021 ccatcgtgag aggcgaggaa gttaaaccag gtggtgttgc tggaaggtag tgtcaggttt
    37081 tgcgcccacg cacaaagcgc ctcaacgttc tgtttttgca ccgcatgcag caccagtggc
    37141 ggcagcgaga actggtacac catatgtgct tcgtcatcgc ctgcgccaaa gtaagcaatg
    37201 ttgtctttat gcggaacatt ggtctcggta atgatcactg tacctggcgc aacgttatca
    37261 ataatcgacc gtaacagttt gataatcaga tgtgtttttt ccagatggat gcagcttttt
    37321 cccggctctt tccacataaa gccaacggca tccaggcgga catactcagc acctttcgca
    37381 aggtaacaga gtaaaacatc caccatcgcc agcaacactt cagggctacg gtaattcagg
    37441 tcaatttgat cgtcactaaa ggtggtccat aaatggcgcg ttgaatgatc gcgcatctgg
    37501 aatggcgtta ataacggtaa cgcacgcggg cgagtgacgg cgctgagatc ggtttgcggg
    37561 tcaacggcaa taaaaaaatc ttcaaaacct ggatgctgtt gtaaatagtt tttaaaccat
    37621 tcactttttg ccgacatatg gttgcagaca aaatcaaaca ttaaatgact gcattcaccg
    37681 agttgctgaa tatcctgcca ctcccccgct tcactggcga cctgatgata atcaattacc
    37741 gaaaagccat catcagatga ccatggataa aacggcaaca aatgaacatg tgaaaaaatg
    37801 ctttgcagcc attgatggta aaactgatta aatgtgggta atggttttaa atcattgctg
    37861 tgaaattgat cggcataggt aattaacacg acatcacttt catcccagtg ttttttacgt
    37921 cgctgtgtaa ttaatcgttt cgcactctca agacgcgtta caagtttctg taaatgagtt
    37981 gcggtaaatg ttccaccgta gatttcgtcc aggtaatccg taattttctg tttcatgttg
    38041 cctttccgtg gtagcggtcc catgaaacga gagactactc cgcagaaaat aaactgtcaa
    38101 ttgagcacaa gggttttagt cacgcaggaa atcatttttt accagggaaa aagcgtattt
    38161 gtgagctgac gcaaataatc tatggggatg taaataaggt gtgacagaaa aaaaaacggc
    38221 gcataagcgc cgctcatggt gaattctttt aacctttgac cttcggcatt gcgatgtctt
    38281 tcaggccacc ggcgttctca acgtgggtat atcccatctc gctaaggatc tcttttgctt
    38341 gccctgactg gcgtccggca ttgcaataca ctttcacggt gtcgttttta tccggaacgg
    38401 cggtggcaat gcgctctttc acttccttca ggggaatatt gatggccccc tgaacgtgct
    38461 cttgctgata ctgctctgga acacgaacat cgatccagtg ttcagcggcg aaaacgggca
    38521 gtgaaaacac cagtgccaga gcaagtaagc cttttttaaa catgtaagcg tccttctatg
    38581 gatgaaaatt gccatccatt atacgcctga gatttaacaa tttttgcgaa actcccctca
    38641 ccttttataa cgctgtgcca gtttattagc agcccgactt aacagcggtt ccagcgccac
    38701 agccagcaac attttcagcg gtcggcgagc aactgatttt atcgcccagc ccgccacacc
    38761 cgccgggcca tagcgcagtg cggtaagaag taccagcttg cctgctaatt tgaaaccagg
    38821 ctttaccttt tgcccggcct gttgccagcg agtattcata actttcctca cagttgacgg
    38881 aaacggctac gtaacgtgaa agtatcggaa gtgacataac gttccatctc gcgtaaacgc
    38941 gtttcacttg ccgccagttc acggtcgact tcatccagca attcgctgct ggaaggtagc
    39001 tgctcaccaa aggccatgtt gtccggcatt ggatcaagcg caaatgacaa aatgatgtaa
    39061 gcaaccaggg taaacagcgc cagaccgaag aaaatcgaca gcaccaccag gatacgcacc
    39121 agttttgccg gtacatcaaa atagttggca atcccggcgc agacgccgcg gaccatgccc
    39181 tgctgtggaa tacgccataa ttttttattg agattaatgc ccgccattag cgatccctcc
    39241 agttcggatg ttctgcatca agaatagatt ccagcgcctg aatacgttcg cgcatccgtt
    39301 ttgcttcatc agccagttgc gctaatcgct gctgctcact ttgcgacaat tcactgcgac
    39361 cagaacgatt gctgtaatgc agccataacc agatcggtaa aacaaacagc acaaaaatgg
    39421 ttaacggaat agccagaaat agcgcgctca taagtactcc ttagacgatg agcggcggcg
    39481 cgttcggacg ccgcctggat gttattattg attgtcttgc ttcattttgg cttttaattg
    39541 tgccagttgt tcgctgattg catcatcggc tttcagttcg gcaaactgat cgtccagcga
    39601 tttttgttta ccgaagctgt ggctttctgc ttccgcttcc atctggtcaa tacgacgttc
    39661 gaaagattcg aaacgagcca ttgcttcatc cagtttgcca ctgtccagct gacgacgcac
    39721 atcgcgcgac gagtttgccg cctgatgacg taacatcaat gcctgctggc gagcgcgtgt
    39781 ttcgctcaat ttgttttcca gctcaccgat ctctttcttc atgcgtgcca gcgtgtcgtc
    39841 caccagcgtc acttcatgtt ccaggctctt aatcagatcg gtcagtttct gtttttcaat
    39901 taacgctgca cgtgccagat cctctctctc tttcagcagc gccagttcgg ctttttcctg
    39961 ccattcaact tcacgcgccg acgcttgttc aatacggcga gtcagctgtt tcttttctgc
    40021 caacgcgcgc gccgaagtag aacgtacttc aaccagtgta tcttccatct cctggatcat
    40081 cagacgaacc agtttctgtg gatcttccgc tttctctaac agagcgttga tgttggcatt
    40141 cacgatgtcg gcaaagcgag aaaaaatacc cataatgttg tcctcttgat ttctgcgttc
    40201 aggattgtcc tgctgaactg ttaatacaat ttgcgtgcca attttttatc tttttgattt
    40261 ataaagatct gattgaagaa tcaacagcaa catgccagga tgagttagcg aattacacta
    40321 acaagtggcg aatttcatca tggcagaata caaagataat ttacttggtg aggcgaacag
    40381 ctttctcgaa gtgctggaac aggtttcgca tctcgcaccg ctggacaaac cggtgctcat
    40441 catcggcgaa cgcggcaccg gtaaagagct gattgccagc cgcctgcatt atctctcctc
    40501 ccgttggcaa gggccgttta tttcccttaa ctgcgcggcg ttaaatgaaa atctgctgga
    40561 ttccgaactg tttggtcacg aagcgggggc gtttaccggt gcgcaaaaac gtcatcctgg
    40621 gagatttgaa cgtgccgacg gcggtacgct atttcttgat gaactcgcta cggcaccgat
    40681 gatggtgcag gagaaattat tgcgcgtgat tgagtacggt gaactggagc gcgttggcgg
    40741 cagccagcca ttgcaggtga atgtgcggtt ggtatgcgcg acgaatgccg atctcccggc
    40801 gatggtcaat gaaggcactt ttcgcgctga cctgctcgac cgactggctt ttgatgttgt
    40861 acaactgcca ccactgcgcg agcgcgaaag cgacataatg ttgatggcag aacactttgc
    40921 catccagatg tgtcgggaaa tcaagctgcc tctgttcccg gggtttacgg agcgcgccag
    40981 agaaacattg ctgaattatc gttggccggg aaatattcgt gaattgaaaa acgtggtgga
    41041 acgttcagtg tatcgccacg gcaccagcga ttatccgctt gatgacatca ttattgatcc
    41101 ctttaaacgg cgtccgcctg aagacgctat cgccgtttca gaaaccacct cgcttccaac
    41161 actgccgctg gatttacgtg agtttcagat gcagcaggaa aaagagttgc tgcaactcag
    41221 tttgcaacaa gggaaatata accagaagcg cgcggctgaa ttactggggt taacttatca
    41281 tcagttccgc gcgttgttga aaaagcacca gatttagcga agttgagcaa tacaacttgc
    41341 aggatgcggc gtgaatgctt tattcggcat acataatcaa tacgtcgttt gcaggcctga
    41401 taagcgcagc gcatcagaca ttattgcgtt gggctattaa tcgctcagcg catcctgcaa
    41461 aattttcatt gccgcatcga attgcacatc cgggatggtc agcggataca ggaagcgaat
    41521 cacgttgccg tatgcgccac aggtcagcag gagcagcccc tgcgccagcg cgcgttgctg
    41581 gattttctgt gcaatcgccg ctgacggctt gcccgtttgc ggatcgttaa actctgccgc
    41641 aatcatcgac cccaggccgc gtaccgcagc aatggccgga acgctttctt tggcatcaat
    41701 caacgtgttt ttgagacgct ggcccagttg attcgcgcgt tcgcagagtg attctttgtc
    41761 gataatattg agcaccgcgt gcgcggcagc caccgccagc gggttcccgg cgtaggtgcc
    41821 acccaacccg cccggcgcgg gcgcgtccat aatattcgcg ttaccgacca cgcccgaaag
    41881 cggcatcccg cccgcgaggc ttttcgccat cgtcattaag tccggcttat cggcgtaatg
    41941 atccatggca aacagtttac cggtacgcgc aaagccgctt tgcacttcat cggcaatcat
    42001 cacaataccg tgctcgtcgc acaggcggcg aatggcggca accagctctt ttggcgcaac
    42061 gttgaaaccg ccctcgccct gcaccggttc gaaaataatg gccgcgacct gcttcgcttc
    42121 gatgtctgat ttaaacaagc gttcgatggc gtcgagggag tcctgtgttg aaatgccgtg
    42181 taaatctgac ggataaggta cgtgatacac cgaaccaggg aacgggccga agccgatttt
    42241 gtacggcgca acttttccgg tcagcgccat ggtcatatat gtacgaccgt ggaagccacc
    42301 gctaaacgca atcacgccag gtcgtccggt atgggcgcga gcaattttca ccgcgttttc
    42361 caccgcttcc gcaccggtag tgaagaatgc ggttttagcc tgcccgctca ctggggcaag
    42421 ggcgttaatt ttctccgcca gcgtgacgta gctttcgtac ggcacaatct gatacgccgt
    42481 gtgggtaaac tgttgcaatt gctgctccac cgccgcgacc agatcagggt ggcgatgccc
    42541 ggtattcagc accgcaatgc ctgcggcgaa atcgatgtat tcgttgccct ctacgtcctt
    42601 cagcgtagcg ttttctgccg actgggcgaa gaagttacac atcacgccaa ccccgcgtgg
    42661 ggtagcagaa agacgacgct gatggaattc attgttgctc atgtttcaca ccttttgact
    42721 cttcactgtt aatcggaaat taaacgccca gacgatcgcg caggctgtaa taagccgcgc
    42781 ccatcgcggt aaatggcacg cgcagcgtgc gcccgccggg gaacgggtaa tgcggcagat
    42841 tggcgaaggc atcgaaacgt tcggcgtcgc cgcgcagcag ttcggcaatc agacgcccgg
    42901 caagatgggt acaggtgacg ccgtggccgc tgtagccctg catgtaatag atgttggtat
    42961 caaggcgacc gaactgcggc atgcgcgaca aggtcagcag gaagttgccc gtccagcggt
    43021 aatcaatttt cacgcccttc agctgcggga aggttttcag cagtttcggc accacaaggc
    43081 gctcaacgtc atccgggtcg cgcgcgccgt agaccacgcc gccgccgtac agcaggcggt
    43141 tgtcggcggt aagacggtag taatcaagca gatagttaca gtcttccaca cagtagtttt
    43201 tcgggatcag cgaacgggct aaatcttccg acagccgttc ggtggtgatc acctgcgtgc
    43261 cgcacggcat gctgcgtttc gccagttccg gctctacttt atcgcccaga tacgcattcc
    43321 cggcgacaat cacatacttc gccgtcacct gacctttggc agttcgcaca acggctggcg
    43381 tggtgtgctg gatctgcgtc acggcagaaa gttcatacac gcgcccgccg ttgaggcgga
    43441 tggcgtccgc ttcgccaatg gcgaggttta gcggatgaat atgcccaccg ctgtgatcca
    43501 gcagcgcacc ggtatagcga tcgctggcta cttcacggcg aatggcgttg gcgtccagca
    43561 attccagctg tttattgccg tagcgttccc agttctcttt ctgctcttca agtgtggcga
    43621 gctgtttatc attcatcgcc acaaacaggc cgccggggcg gtagtcgcag tcaatctgat
    43681 aacgtttgat acgttcgcgg atgatctcac caccttcgaa catcatgctg ccgagcatac
    43741 gggcggtgtc catgccgtag cttttttcga tcacgtcgat gtcgcggctg taggagttca
    43801 caagctgccc gccattgcgc ccgcttgcgc caaagccgat gcgtgaggct tcgagaacca
    43861 ctacgtcaaa gcccgcttcc gccagatgca gcgcggaaga gagcccggta tagccgccgc
    43921 cgaccacgca aacgtcgcag gaaatcgact cattcagcgt gtcgaatggc gcgtatttat
    43981 tcgcactggc ggcgtagtaa ctgcttgtat gttcggtcat ttcaggcctc caggcttatc
    44041 cagatggttt tcagttcggt gaatttttca agggcatgca gggatttgtc gcgaccgttg
    44101 ccgctctgct tatagccgcc aaacggcacg gtcatatcgc cgtcgttgta gttattgacg
    44161 aagacggaac cagccttcag gcggcggctc atgcggtgcg cgcgggagag gtcgcgcgtc
    44221 cataccgccg cgccaaggcc gtactggctg tcgttggcaa gctgtagcgc ctggtcttct
    44281 gatgtgaaac gcgtgaccac cagcaccgga ccgaaaatct cttcgcggct taaggacgca
    44341 ttcgggtcta catccacaaa gatggtcggg ccgatggcgg cagccagctc agcgttacga
    44401 ccatccagca gcagttgccc tttgctttcg ccttcccgaa taaagctatg gaccgagtcg
    44461 gcgtgggcgc agtcgattaa ggtgcccatg gtggttgcgg gatcaagtgg atggcccggc
    44521 tgccagtttt gcgcctgctg ttttaacagg gctaagaatt catcggcgat gctctcttcc
    44581 agcaacaaac gcgttccggc gatgcacacc tggccctggt tgtagaagat gcctgcggcg
    44641 gtggcgcttg ccgctttttg cagatccgga caatcggcga aaacgaggtt ggcgcttttg
    44701 ccgcccgctt ccagccagac gcgtttcatg ttgctgtcgc ccgcatcttt cagcagctgt
    44761 ttcccggtac gggttgaacc ggtaaaggca atggcgtcga tatcgttatg acgcgacagc
    44821 gcctgccccg cttcatgacc aaaacccgtc accacgttca acacaccatc cggcaagcct
    44881 gcttctttcg ccagccccgc gagacgaatc gcactgagcg gtgatttttc agacggtttt
    44941 agaatcacgc tgtttcccgc cgccagcgcc gggccgagtt tccagcaagt cagcaacagc
    45001 gggaagttcc acggcacgat ggcggcaatc acgccgaccg gttcacgcac gatcatcgcc
    45061 agctcatggc tactggtggt cgccacttcg ccatacactt tgtcgatcgc ttcggcgtac
    45121 cagcgaatgg cgcgcgccgc gccgggaata tcatcacgca gactgtgacg aatcggtttg
    45181 ccggtgtcga gagtttccag cagtgccagc tcttcggcgt gggcttccat taaatcggcg
    45241 agtttattca gtaccgcttt acgtttagcc ggagaagaga gtgaccagtc gccgcgttca
    45301 aatacgccgc gtgctgcgct caccgcacgg tcgatatcga cgctcttgcc gcgggcaatt
    45361 ttcgccagcg gtgcctgggt gaccggatca acggtttcaa aggtttcatt ttccgccgca
    45421 gcagtatatt caccgttaat aaataagcgg ttttcaatgg cgagacttaa cgctttatcc
    45481 tgccagtaag ccagatgatg aaaattcatt atgactcctg tttcacgtct atcagatata
    45541 tgcagtttgt gaacgtcagg gcgcagagat ccccgggccg tatgcccgtt tatttctggc
    45601 gacattcttt atatacactt catccttcaa gccgcctctg ccttggctgc gttcgctccc
    45661 ccgaatcact tacctgagta agctcatcgg gattcgctca cttgccgcct tgatgcaact
    45721 tgaatgattt tgtgtataga ttgccgggcg cattgcgctt acccggctta cagaacaaaa
    45781 agattaaaac gtggtgggcg tatgggcgct gataattcgg caaatacctg ccgacgtatt
    45841 actgaaactg tgcgggatgc cggtattaat ggcataactt tgccccgcga cgaggtggta
    45901 atcctgacca ttaatcgtca gaacaatttc accttccagt actgtgccta tttcctcacc
    45961 ctgatgctta attctttccc cagtggttgt gcccggctgg tacgtttcaa agatcatcgc
    46021 cagcgtgcga ttcgggttac cattatgaac cagcttcatt gacacaccct gactgcccat
    46081 ctcaattaag tcgtcctgat taatgacgac ctgcggctca tcaggttttt ccggctcgga
    46141 aaagaattcc gagagtgaca gaccatacac cttcagcagc ttttgcagcg tactgatggc
    46201 agggctgact ttatcttgtt ctatcgtact gatagcactg tgagtcagcc cggagagttc
    46261 ggcggcacga cgttgtgaaa gcccctgctg ctggcggatt tccgacaagc gttttcctgg
    46321 cgccagtccc tcatcactca tatttgcatt tccttaaact gtagtggtca gagtcgctgt
    46381 ttttcagcga tatggtgctg acaagcggtg ataaagccct cgaacaatat acgcgaaagc
    46441 gcgtactcgc tactgttcca ttccgggtgc cactgtacgc ccagcgcaaa aggatgattg
    46501 atgacgctaa ccgcctcaac taagccatcc ggcgagcgag cttcaacacg caaccgtggg
    46561 ctaacgacct tcgccccttg cccatgcaga gagtttaccc aaaagttgct acattcaggt
    46621 aacaacgcag acagtaatcc cccctcttca acctgaactt catgcgacgg tgcatattgc
    46681 tgttccaccg gcagttcggg atcttcccga tgttccagca attcaggctg ttcgcacagc
    46741 ttgcgatgca acgaaccacc ggttgccacc accagttcct gtaaaccccg gcagatggcg
    46801 aaaatgggga tgcgcctttc gagtgcggca ttaattaacg ccatgctcag aagatcacgc
    46861 ccgggatcgg cgtcaggctc atcgccgttt tcaccatata ggtgcggctg cacattgctg
    46921 ggactaccag gaagataaat gccatcgagt ttcggcaaaa gttgttcaag taatgacggt
    46981 tccgccagcg catgtggtag cgcaattggc aagccgcctg cgtggatgat ggcattcagg
    47041 tacttttctt gcagagtctg ggtcgcatga tccttaagcc tgttcctgca cattacgaca
    47101 ccgataaccg gattgttcat tatattttcc acgctcaatc tcacaaagtg gactaaatta
    47161 tcgccattac tgcggtttaa atgaccttta tgttcaatat tttttcaatc tagcagtggg
    47221 ttttcatttt tgcaaactca atttaacatt tgacaaacat ttggtttgca tacagattcg
    47281 aatggtggtc attatatttt acgctttgat aacgagcgga aaacaaacca aaggcgaaga
    47341 atcatggaaa ccaatatcgt tgaagtagag aactttgttc agcagtcaga agagaggcgg
    47401 ggtagcgcct ttacgcagga agtgaaacgc tacctggagc gctacccgaa tacgcaatat
    47461 gttgatgttt tacttactga tttgaatggt tgcttccgtg gaaaacgtat ccctgtttca
    47521 agcctgaaga agttggagaa agggtgttat ttcccggcct cggtgtttgc aatggatatt
    47581 ctgggcaacg tagtagaaga agcgggtctg ggtcaggaaa tgggcgagcc ggatcgtact
    47641 tgtgttcctg ttctcggttc cttaacccct tctgccgccg atccagagtt tatcggtcag
    47701 atgctcctga ccatggtcga tgaagatggc gctccctttg acgttgagcc gcggaacgtt
    47761 cttaaccgcc tgtggcagca gctacgccag cgcggattgt tcccggttgt agcggtagag
    47821 ctggagttct atttactgga tcgtcagcgc gacgctgaag ggtatctgca accgccctgc
    47881 gcgcccggca ccgatgaccg caatacacaa agccaggttt actccgttga taacctcaac
    47941 cacttcgccg acgtgctcaa tgatattgat gaactggcgc agttacagtt aattcccgcc
    48001 gacggtgcgg tcgccgaagc ctcgccgggt cagtttgaaa tcaaccttta ccataccgat
    48061 aacgtgctgg aagcctgcga tgatgcgctg gcgctaaaac gcctggtgcg tctgatggca
    48121 gaaaagcata agatgcacgc cacttttatg gcgaagccgt atgaagagca cgcgggcagc
    48181 gggatgcata tccatatcag tatgcaaaat aatcgtggcg agaatgtgct ttctgacaca
    48241 gaaggcgaag attcgccgct gctgaaaaag atgctcgccg ggatgattga cctgatgccg
    48301 tcgtcgatgg cgttgctggc accaaacgtg aactcgtatc gccgcttcca gccgggaatg
    48361 tatgtgccga cgcaggcgtc gtggggccat aacaaccgca ccgtcgccct gcgtattccg
    48421 tgcggcgacc gtcataatca ccgcgtggaa tatcgcgtgg cgggtgccga tgccaaccca
    48481 tatctggtga tggcagcgat ttttgccggt attttgcatg gccttgataa cgagctgccg
    48541 ttgcaggaag aagtcgaagg caacgggctg gaacaggaag gcttaccctt cccgattcgc
    48601 cagagcgatg ccctaggtga gtttatcgag aatgatcacc tgcgccgcta tttaggcgaa
    48661 cggttttgcc atgtgtatca cgcctgtaaa aatgatgagc tgttgcagtt tgagcgcctc
    48721 atcacagaaa cagaaattga gtggatgttg aaaaacgcgt aattccccat gcggggttgt
    48781 ttcatcggcc tgagcggagc caggcctgac atttgctggt aatccagaca tttcccggat
    48841 gtcgcgttat ggagcgcagg cggcaacggg cttccgttaa cgattaagac tgtgacgagg
    48901 aaatgagatg atgccgatga ttaaatcccc gcatggcgag ggaggctgcg tatgcgcccc
    48961 tcctgcgact gactggactc cgcccccttt gcttcccttg ctaaacaggt ttgatttcag
    49021 gtcgacacga ccgcaaacct tattacgcag gggaggcagc aattatggct attaattcac
    49081 cactgaatat tgctgcgcaa cccggcaaaa cccgtctgcg aaaatcactg aaattgtggc
    49141 aggtggtaat gatgggtctg gcctatctca cgccgatgac cgtatttgat acttttggta
    49201 ttgtctccgg cattagcgac ggtcacgttc cggcgtccta tttgctggcg ctggcgggcg
    49261 tgctgtttac cgctatcagc tacggcaaac tggttcgcca gtttccggag gccggttcgg
    49321 cctataccta cgcgcaaaag tcgattaatc cgcacgtcgg atttatggtc ggctggtcat
    49381 cactgctgga ttatctcttt ttgccaatga tcaacgtttt gttggcgaaa atctatctct
    49441 ccgccctctt cccggaagtg ccgccgtggg tgtgggtggt aaccttcgtc gccattttaa
    49501 ccgccgcgaa tctgaagagc gtcaacctgg tcgctaactt caataccctg tttgtactgg
    49561 tgcaaatctc catcatggtg gtgtttatct tcctggtggt tcagggactg cataaaggag
    49621 aaggcgttgg caccgtctgg tcacttcagc cgtttatcag cgagaacgcg cacctgatcc
    49681 cgattattac cggtgcgacg attgtctgtt tctcgttcct cggtttcgat gcggtgacca
    49741 cgctttcgga agagacgccg gacgccgcac gcgtgatccc gaaagccatc ttcctgacgg
    49801 cggtatacgg cggcgttatc tttatcgcgg cgtcgttctt tatgcagctg ttctttcccg
    49861 atatcagccg ctttaaagac ccggacgccg cactgcctga aattgcgctg tacgtcggcg
    49921 gcaagctctt tcagtcgatt ttcctctgca ccacgtttgt gaacacatta gcgtctggcc
    49981 tggcttcaca tgccagcgtg tcgcgtctgc tgtatgtgat ggggcgtgac aatgtgtttc
    50041 cggagcgcgt gtttggctat gtgcacccaa aatggcggac tccggcactc aacgtcatta
    50101 tggtcgggat tgtcgcgctg tcggcgctgt tctttgattt agtcaccgcg acagcactga
    50161 ttaacttcgg tgctctggtg gcgtttacct tcgtgaatct gtcggtgttt aatcatttct
    50221 ggcggcgtaa aggaatgaat aaaagctgga aggatcactt ccactatttg ctgatgccgc
    50281 tggttggcgc gctgacggtg ggtgtgctgt gggttaacct agagtcaacg tcactgacac
    50341 tcggtctggt atgggcttcg ttaggcggcg catatttgtg gtatctgatc cgccgctatc
    50401 gcaaagtgcc gctgtacgaa ggtgacagaa cgccggtgag cgaaacgtaa tagccggatg
    50461 cgcggcgcat tatcctgcct gagggtaacg cgccgcgcca ttaactataa aaagatgtat
    50521 gacccggatc acgagtgcta tcatccgccc ttatgcttag cgcattaacc ccaggggcaa
    50581 ataatgaacc acgatatccc gctaaaatat tttgatattg ccgatgagta cgcgaccgaa
    50641 tgcgcagagc ctgtcgcaga ggctgaacgc acaccgctgg cccactactt ccagttactg
    50701 ctcacccgtt tgatgaacaa tgaagagatc agcgaagaag cccaacatga aatggctgct
    50761 gaagcgggga ttaatcccgt acgcattgat gagatcgcag agtttttgaa tcaatggggt
    50821 aatgagtaat tatttaattg cagacgatcg gtcagtatcg ttgttcggat aaagcgttca
    50881 cgccgcatcc ggctaacaat gacacgcact gcagcgtgct ttaatgggtt tctcacgatc
    50941 gtaccgctct gctcctcgct aagttgttct gtgagttata gttatagaga aatcacgggt
    51001 acgaatgttg aacagatggc gacgccacct gttcaacgac caaccacacg gccctcatta
    51061 cgacccctga acacaaacgg taatggcaaa tgtcattaaa tattagtaag atagcctctt
    51121 tacataaaat cccttaatat tatgcgccag gtattatcgt ctcttttggt gattgctgga
    51181 cttgtgagtg gtcaggcaat cgccgcgcct gaatcccccc cgcatgctga tatccgcgac
    51241 agcggttttg tctattgcgt cagcgggcaa gtcaacacct ttaacccatc caaagcgagc
    51301 agtgggttaa ttgtcgatac ccttgccgcc cagttttatg atcgactgct ggatgtcgat
    51361 ccctatacct atcgcctgat gccggaactt gccgaaagct gggaagtact cgacaacggc
    51421 gcgacctatc gcttccacct gcgtcgcgat gttccgtttc aaaaaaccga ctggtttact
    51481 cccactcgta aaatgaatgc cgacgatgtg gtgtttacct tccagcgaat ttttgaccgc
    51541 aacaacccgt ggcataacgt caacggcagc aacttcccct acttcgacag cctgcaattt
    51601 gccgataacg tgaaaagcgt ccgcaaactg gataatcata ccgttgagtt ccgactcgcc
    51661 cagccggatg cttctttttt gtggcacctc gcaacccatt atgcttcggt catgtcggca
    51721 gaatatgccc ggaagttaga gaaagaagat cgccaggagc aactcgaccg tcaaccggtc
    51781 ggcaccggtc cgtatcagtt gtcggaatac cgcgccgggc aatttattcg cctacaacgt
    51841 catgatgact tctggcgcgg taaaccgtta atgccgcagg tagtggtgga tttaggctcc
    51901 ggcggcaccg gacgtctgtc gaaactcctg accggggaat gcgacgttct ggcctggcct
    51961 gctgccagcc agctatccat tttgcgtgac gacccgcgct tgcgtttaac gctgcgtcct
    52021 gggatgaacg tcgcctatct ggcatttaac accgccaaac cgccgctaaa taatcccgct
    52081 gtccgccatg cactggcact ggcgattaat aaccagcgcc tgatgcaatc catctattat
    52141 ggtacggctg aaacggcggc ctctatttta ccgcgcgcct cgtgggccta tgacaacgag
    52201 gctaaaatta ctgaatacaa tccggcgaaa tcgcgcgaac agttgaaggc gttggggctg
    52261 gaaaacttaa cgctgaaact gtgggtgccc acacgttcgc aggcgtggaa ccccagtcca
    52321 ctgaaaactg ccgaactgat tcaggcggat atggcgcagg ttggcgtaaa agtggtgatt
    52381 gtgccggtag aaggtcgctt tcaggaggcg cggttgatgg atatgagcca tgatctgacg
    52441 ttatccggtt gggcgacgga cagtaacgac ccggacagtt tcttccgtcc tttactgagc
    52501 tgcgcggcaa ttcattcaca gaccaacctc gcccactggt gcgatccgaa attcgacagc
    52561 gtgttgcgta aggcgctctc ctcgcagcag ctggcggcgc gtattgaagc ctatgacgaa
    52621 gcgcagagta ttctggcgca ggaattgccc attttgccgc tggcgtcgtc attgcgtttg
    52681 caggcctatc ggtacgatat caaaggtctg gtacttagcc cgtttggtaa cgcctccttt
    52741 gctggggtgt atcgcgagaa acaggatgag gtgaaaaaac catgattatc ttcaccttac
    52801 gccgcatttt gttattgatt gtcaccttgt tcctgctgac cttcgttggc ttcagcttga
    52861 gctatttcac ccctcatgcg ccgttacaag gcgcgtcact gtggaatgcc tgggtgttct
    52921 ggtttaacgg cctgatccac tgggattttg gtgtttccag cattaacggt caaccgattg
    52981 ccgaacagtt aaaagaggtc ttcccggcga cgatggaact gtgcatcctc gccttcggtt
    53041 ttgcgctgat tgttggcatc cccgtgggga tgattgccgg gattacgcgc cataagtggc
    53101 aggataactt gatcaacgcc atcgccttac tgggcttttc aatcccggtg ttctggctgg
    53161 cgcttctgtt gacgctgttt tgttcactca cgctaggttg gttgcccgtt tcagggcgtt
    53221 tcgatctgct ctacgaagtg aaaccgatta ccggttttgc gttgattgat gcctggcttt
    53281 cggactcacc atggcgggat gaaatgatca tgagcgcaat ccgccatatg atattgcccg
    53341 tgatcaccct gtcggttgcg ccaacaactg aagtgatccg cctgatgcgt atcagcacta
    53401 tcgaagtgta tgaccagaac tatgtaaaag cggcggcgac ccgcggtttg tcgcgcttta
    53461 ctattttgcg tcgccacgtt ctgcataacg cgttaccgcc ggttattcct cgcctgggct
    53521 tacagttttc caccatgctg acgctggcga tgatcaccga aatggttttt agctggccag
    53581 gtttagggcg ctggttaatt aacgccatcc gccagcagga ttacgcagcc atttccgccg
    53641 gagtgatggt gtgtggctca ctggtcatta ttgttaacgt gatttctgat attttgggtg
    53701 ccatggctaa ccctctgaaa cataaggaat ggtatgcctt acgatagcgt atacagcgaa
    53761 aagcgcccgc cgggcacgct gcgtaccgcc tggcgcaaat tttatagtga cgcctctgcg
    53821 atggtcggcc tgtacggctg cgcggggctg gctgtactgt gtatttttgg cggctggttt
    53881 gcgccttacg gcatcgacca gcaatttctc ggttatcaat tattgccgcc gtcatggtcg
    53941 cgctatggcg aagtttcttt cttcctgggg actgacgacc tcggacgcga tgttttaagc
    54001 cgtttactga gcggagctgc gccaaccgtg ggtggcgcat ttgtggtgac gcttgccgcg
    54061 acgatctgtg gcctggtact gggcaccttc gccggggcga cacacggcct ccgctcagcg
    54121 gtgcttaacc atattctgga taccttactg gcgatccctt cgctgctgtt ggccattatc
    54181 gttgtagcgt ttgccggacc gagtttgtcc cacgctatgt ttgctgtctg gctggcattg
    54241 ctgccgcgta tggtacgttc gatttacagc atggtgcatg acgaactgga aaaagagtac
    54301 gttatcgccg cccgtctgga tggcgcatca acgctgaata ttctctggtt tgctgtgatg
    54361 ccaaacatca ccgccgggct ggtcaccgag atcacccgcg cactgtcgat ggcaattctc
    54421 gatatcgccg cgctgggctt tctcgatctc ggcgcacaac tcccctcgcc tgaatgggga
    54481 gcgatgctcg gtgatgcgct ggaactgatt tatgtcgccc cgtggactgt catgctgcca
    54541 ggtgcggcaa ttatgattag cgtgttgttg gttaacctcc ttggcgatgg cgttcgtcgt
    54601 gcgattattg cgggggtgga ataatgccat tactcgatat tcgtaacctg accattgaat
    54661 ttaaaaccgg tgatgagtgg gttaaagccg tcgaccgggt aagtatgacc ttaaccgaag
    54721 gtgaaatccg cggtcttgtt ggtgaatccg gttccggcaa gagtttgatt gcgaaagcga
    54781 tttgtggggt gaataaagat aactggcgtg ttactgctga ccgtatgcgt tttgatgata
    54841 tcgatttgct gcgtctctcc gcacgcgaac ggcgcaaact ggttggccat aacgtgtcga
    54901 tgattttcca ggaaccgcag tcgtgtcttg acccttcaga acgtgtgggt cgccagttga
    54961 tgcaaaacat cccagcctgg acctataaag gccgttggtg gcagcgtttt ggctggcgca
    55021 aacgccgtgc cattgaactg ctgcaccgcg tggggattaa agatcacaaa gatgcgatgc
    55081 gcagttttcc ctatgagttg accgaaggtg aatgtcagaa agtgatgata gccattgcac
    55141 tggcgaatca accgcgtctg ctgattgctg acgaaccgac caactcaatg gagccaacaa
    55201 cccaggcgca aatctttcgc ctgctgacgc gtctcaacca aaacagcaat accactattt
    55261 tgcttatcag ccatgactta caaatgctta gccaatgggc ggataaaatt aacgtgcttt
    55321 actgcggtca aacagtggaa accgcgccga gtaaggagtt ggtgacgatg ccacatcatc
    55381 cttataccca ggcgctgatc cgcgcgatac cagacttcgg cagcgcgatg ccgcataaaa
    55441 gtcgcctcaa tacgctgccc ggcgctatcc cactgctgga acagttaccg attggttgtc
    55501 gtctggggcc acgttgcccg tatgcacaac gagaatgcat tgtgacgcca cgtttgacgg
    55561 gggcgaaaaa tcatctctat gcctgtcatt tcccgctgaa catggagaaa gagtgagatg
    55621 atcgaaacgc tgcttgaagt gcgtaattta agtaaaacgt tccgctaccg gaccggctgg
    55681 tttcgtcgtc agaccgtaga agcggtaaaa cccttgagct ttacgctacg tgaaggccag
    55741 acactggcga ttattggcga gaatggttcg ggtaaatcca cgctggcaaa aatgctggcg
    55801 ggaatgatag agcctaccag cggcgagtta ttgattgacg atcatccact gcattttggc
    55861 gattattcct tccgcagtca gcgtattcgc atgatttttc aggatccttc gacctcgttg
    55921 aatccccgtc aacgtatctc gcaaattctg gattttccac tgcgcctgaa caccgacctg
    55981 gaaccggaac agcgtcgcaa acagattatt gaaacgatgc gtatggttgg gctgctaccg
    56041 gatcacgtca gttactatcc gcatatgttg gcacccggac aaaaacagcg tctgggtctg
    56101 gcgcgcgcgc tgatattgcg cccaaaagtc attattgccg atgaagccct cgcttcactg
    56161 gatatgtcga tgcgttcgca gttgattaat ctgatgctgg aattacagga aaaacagggc
    56221 atttcgtata tttatgttac ccagcatatc ggaatgatga agcacattag cgaccaggtg
    56281 ctggtgatgc atcagggcga ggttgtcgag cgaggcagca ccgcggatgt gctggcatcg
    56341 ccgctgcatg aactcaccaa acggctgatt gccgggcact ttggcgaagc attgacagcg
    56401 gatgcgtggc gtaaagatcg ctaattttct gaatcctcct tccagattat ccacctcatt
    56461 ctgacaagag attaaatctt gtcagaatga ggtgatggat aaaataaaat caaatgcgcg
    56521 tgatttaaga cgcaatctca ctttgcagga acgaaagctc tggcgatatc ttcgcagccg
    56581 acgttttggt gatttcaaat ttcgccgtca acatccggtg gggagctaca ttctcgattt
    56641 tgcttgctgc tcggcgcgcg tagtcgttga gctggatggt gggcagcatg atttggcagt
    56701 tgcctatgat accaggcgca ctagctggct tgagtcgcag aactggaccg tgctgcgttt
    56761 ctggcataac gagattgatt gtaatgagga ggcggtgctg gagattattc tgcaggaact
    56821 gaaccgccgg tcaccctcac cctgaaaggg cgagggggcc gaccgagccg aatagctgtt
    56881 gtggtgaaaa catggagaca gtgctggaga atattcggca aggtctgaac catcccagcc
    56941 atcgccataa aagggttagg ggctgtatga gcctgtttgt tgctggggta acaatatttg
    57001 cacaatatgg tcccctcgcc cctctgggga gagggttagg gtaaggggaa aagcgcccac
    57061 cctgccgcag cctgctccgg tcggacctgg caactatagc tactcacagc caggttgatt
    57121 ataataaccg tttatctgtt cgtactgttt actaaaacga cgaatcgcct gattttcagg
    57181 cacaacaagc atcaacaata aggattaaag ctatgggttt tctttccggt aagcgcattc
    57241 tggtaaccgg tgttgccagc aaactatcca tcgcctacgg tatcgctcag gcgatgcacc
    57301 gcgaaggagc tgaactggca ttcacctacc agaacgacaa actgaaaggc cgcgtagaag
    57361 aatttgccgc tcaattgggt tctgacatcg ttctgcagtg cgatgttgca gaagatgcca
    57421 gcatcgacac catgttcgct gaactgggga aagtttggcc gaaatttgac ggtttcgtac
    57481 actctattgg ttttgcacct ggcgatcagc tggatggtga ctatgttaac gccgttaccc
    57541 gtgaaggctt caaaattgcc cacgacatca gctcctacag cttcgttgca atggcaaaag
    57601 cttgccgctc catgctgaat ccgggttctg ccctgctgac cctttcctac cttggcgctg
    57661 agcgcgctat cccgaactac aacgttatgg gtctggcaaa agcgtctctg gaagcgaacg
    57721 tgcgctatat ggcgaacgcg atgggtccgg aaggtgtgcg tgttaacgcc atctctgctg
    57781 gtccgatccg tactctggcg gcctccggta tcaaagactt ccgcaaaatg ctggctcatt
    57841 gcgaagccgt taccccgatt cgccgtaccg ttactattga agatgtgggt aactccgcgg
    57901 cattcctgtg ctccgatctc tctgccggta tctccggtga agtggtccac gttgacggcg
    57961 gtttcagcat cgctgcaatg aacgaactcg aattgaaata atcattctgt tggtaaatat
    58021 tgggcggcgt tctgccgccc gttatctctg ttataccttt ctgatatttg ttatcgccga
    58081 tccgtctttc tccccttccc gccttgcgtc aggataacga tttcctttac gaccaaggag
    58141 cgcccatgga acaacgccac atcaccggca aaagccactg gtatcatgaa acgcaatcca
    58201 gtactgcgga gtatgacgtt ctgcctctgg tcccggaagc cgcaaaggtc agcgatccct
    58261 ttctgctcga cgtgatcctt gatgaagaaa cgctggctcc cttcctttca tggctggtcc
    58321 ctgcgcgcgt tcttgcagtg gaattgttcc ctgaccagct taccgtgacc cgttcacaga
    58381 ccttcaccgc ttatgaacgc ttgtctacgg ccctgacggt tgctcaggtt tgcggcgtcc
    58441 agcggttatg taactactat tcggcgcgac ttacgccgct ccccgggcct gattcctcca
    58501 gggaaagcaa tcataggttg gcacaaatca cgcaatatgc ccgccaactg gctagctcgc
    58561 cttctattat cgacaaccga tcgcgccagc atctgaatga cgtcggtctt actgccgggg
    58621 actgtgtgat cattaaccaa atcattggtt ttattggctt tcaggcgcgg accattgcga
    58681 catttcaggc ttatctcggg cacccggtac gctggttacc cggtctggaa atacaaaact
    58741 acgccgacgc gtcactgttt gctgatgaat caatacgctg gcgaagcagc tatgaggtgg
    58801 aaaaactacc tgaagagtac acaaaaagtt caacagcaga actttgccaa ctggccgaaa
    58861 cactctctct ccaccctatt tcactttccc ttctcgaaag gttgttaaac agtacacggg
    58921 ttaatacaca gccggataat aagcttgcgg cgttgttatg cgcgcggata aatggcagtc
    58981 ctgcttgttt tgccgcctgt atggattcat caaatgaata taaaaaaatc agcccccttc
    59041 tgcgcaaggg cgaaaatgaa attaaccaat gggctgaccg tcattctgtt gagcacgcta
    59101 ccgttcaggc gatacaatgg ctgacccgag cacccgatcg ctttagcgcc gcccagttca
    59161 gccctttact cgaacacgaa aaatcatcaa cgcagattat taatctgctg gtatggagcg
    59221 ggctgtgtgg ctggataaat cgcttaaaaa tcgcgttggg tgagacatat taaccttgcc
    59281 gcgtcagaca gattcgcgta aaactgtcag ccgctctaat ggccaccaaa atagacaatt
    59341 atgtttcagg acaacccgct gctagcgcag cttaaacagc aactgcattc ccagacgcca
    59401 cgcgctgaag gggtggtaaa agccacagaa aaaggctttg gcttcctgga agtcgacgcg
    59461 caaaaaagtt atttcattcc gccgccgcag atgaaaaaag tcatgcatgg cgaccgaatt
    59521 atcgcggtga tccacagtga aaaagaacgt gaatccgcag agccagaaga actggttgaa
    59581 ccgttcctga ctcgtttcgt gggtaaagtt cagggcaaaa atgaccgtct ggccatcgtt
    59641 cctgatcatc cactcttaaa agacgccatt ccttgccgcg cagcccgtgg cctgaaccac
    59701 gagtttaaag aaggcgactg ggcggttgcc gaaatgcgcc gtcatccgct gaaaggcgat
    59761 cgttctttct atgctgaact gacacaatac atcacttttg gtgacgacca ctttgtaccg
    59821 tggtgggtta cccttgcgcg ccataatctg gaaaaagaag caccagacgg cgtcgctacc
    59881 gaaatgctcg atgaaggtct ggttcgtgaa gatctgaccg cgctggattt tgtcaccatc
    59941 gacagtgcca gcacagaaga tatggatgac gcccttttcg ctaaggcgtt gccggatgac
    60001 aaacttcagc tgattgtggc gattgccgat ccaaccgcgt ggattgctga aggcagcaag
    60061 ctggacaaag ccgcgaaaat tcgcgcattc accaactatc tgcctggctt caacatccct
    60121 atgctgcctc gcgagctttc tgacgatctc tgctcactgc gcgccaatga agtccgcccg
    60181 gtactggcat gccgcatgac gctctccgct gatggcacca ttgaagataa tatcgaattc
    60241 tttgccgcca ccatcgaatc caaagcaaag ctggtgtatg accaggtttc tgactggctg
    60301 gaaaataccg gtgactggca gcctgaaagt gaagcaattg ccgaacaagt ccgtttgcta
    60361 gcgcaaattt gccaacgccg cggcgagtgg cgtcataacc acgcactggt gtttaaagat
    60421 cgcccggatt accgctttat tctcggtgaa aaaggtgaag tgctggatat cgtcgccgag
    60481 cctcgtcgca ttgccaaccg tatcgtcgaa gaagcgatga ttgccgctaa catttgtgcg
    60541 gcccgcgtac tgcgcgataa gctcggtttt ggcatctata acgtgcatat gggctttgat
    60601 ccggcgaatg ccgacgcgct ggcagcgttg ctgaaaacgc acggtctgca tgtcgatgcc
    60661 gaagaagtgc tcacgctgga cggtttctgc aaactgcgtc gtgaactgga cgcgcaacca
    60721 actggtttcc tcgacagccg cattcgtcgc ttccagtcat ttgctgaaat tagcactgaa
    60781 cccggtcctc actttggcct cggtctggaa gcatacgcca cctggacttc gccgatccgt
    60841 aaatatggcg acatgatcaa ccaccgtctg ctgaaagcgg ttatcaaagg cgaaactgcg
    60901 acgcgcccac aggatgaaat cactgtccaa atggccgagc gtcgccgtct caaccggatg
    60961 gcagaacgtg atgttggtga ctggttatac gcacgcttcc tgaaagacaa agccgggacc
    61021 gacacccgtt tcgcagcgga aattgtcgat atcagccgtg gcggcatgcg tgttcgtttg
    61081 gttgataacg gcgctatcgc ctttattccg gcacctttct tacacgctgt gcgcgatgaa
    61141 ctggtttgca gccaggaaaa cggcactgta caaattaaag gtgaaacggt ttacaaagtg
    61201 actgacgtta ttgacgtcac tattgccgaa gtccgcatgg aaacccgcag cattattgcg
    61261 cgcccggtcg cgtaatctcc tttcacggcc cattcctcat ggatgggccg tttatttccc
    61321 cgctctacct tcgttatatc caggcagatt ttttaactga ctttcgtttg aaaactggcg
    61381 tttttccaaa actggattac aaataaatat ataaaaaaaa caatgcgttt agccgccgtt
    61441 aatttcatgt aagtcatgtt gttcctttta tttaaccgcg gactccgcag ttaaccggag
    61501 gatatgcatc atgaaaaccg ttagggagtc cacaacgttg tacaactttc tcggatcgca
    61561 caatccatac tggcggttga cggaaagcag cgatgttttg cgcttttcta ccaccgaaac
    61621 cacagaacct gatcgtactt tgcagttatc tgccgaacag gctgctcgca tcagggaaat
    61681 gacggtcatc acctccagcc tgatgatgag tctgaccgtc gatgagagcg atctttctgt
    61741 gcatctggta ggacgaaaaa tcaataaacg ggaatgggct ggcaacgcgt ctgcatggca
    61801 tgacacaccg gcggttgctc gtgatttatc acacgggctt tcctttgctg agcaggtagt
    61861 ttctgaagca cattccgcaa tagtcattct cgacagccgg gggaatattc aacgcttcaa
    61921 tcggttatgt gaagattaca cagggttgaa agaacacgac gtcattgggc aaagcgtgtt
    61981 taaactgttt atgagccgtc gtgaagctgc ggcatccagg cgcaataacc gtgtattttt
    62041 tcgaagcggc aatgcatatg aagtcgaact gtggatacca acatgtaaag gccagcggct
    62101 gtttctgttt cgcaataaat ttgtccacag cggcagtggc aaaaacgaga tttttttaat
    62161 ctgttccggc accgacatta ccgaagagcg ccgcgctcag gagcgactgc gtattctggc
    62221 aaataccgac agtatcaccg gactgccgaa tcgtaacgca atgcaggatt taatcgatca
    62281 cgctattaat catgcagata acaataaagt tggggttgtg tatcttgatt tggataattt
    62341 caaaaaggtc aatgacgcct atgggcattt gtttggtgac cagttattac gcgacgtgtc
    62401 attggctatt ttaagctgtc tcgaacatga ccaggtgttg gcgcgtccag gtggggatga
    62461 gtttctggta ctggcatcca acacctcaca aagcgcgctg gaagcaatgg catcacgaat
    62521 tttgacccgc ttacggctcc cctttcgcat tggtttaatt gaagtttata ccagctgttc
    62581 agtaggtatt gcactctctc ccgaacatgg ttcagacagc acggctatta ttcgtcacgc
    62641 cgacacagca atgtacacag cgaaggaagg cggacgagga caattttgcg tttttactcc
    62701 agaaatgaat cagcgggtat ttgaatatct ctggctggat accaacttgc gtaaagcact
    62761 ggaaaacgat cagttggtta ttcactatca accgaaaatc acctggcgtg gcgaagtgcg
    62821 cagtctggaa gcactagtac gttggcagtc acctgaacgt gggttgattc caccgttgga
    62881 cttcatttcc tacgccgaag agtcagggct aattgtgcct ttaggccgtt gggtgattct
    62941 cgatgtcgta cgccaggtgg caaagtggcg ggataaaggc ataaacctgc gagtggcggt
    63001 aaatatttct gcacgtcagc tcgccgatca aaccattttc accgccctga aacaggttct
    63061 ccaggaactc aattttgaat actgccctat agatgttgaa ctgacagaga gttgtctgat
    63121 tgagaatgat gaactggcac tgtctgttat tcaacaattt agccaactag gtgcgcaagt
    63181 gcatctggat gattttggta ccggctactc ttcactttcg caactggcgc gctttccgat
    63241 cgatgccatc aaacttgacc aggtttttgt tcgagatatt cacaaacaac ctgtctcgca
    63301 gtcactggtc cgggcgatcg tcgctgtggc ccaggcattg aatcttcagg tgatcgccga
    63361 aggtgtagag agtgcaaagg aagatgcttt tttaaccaag aacgggatca atgagcggca
    63421 aggatttttg tttgccaaac cgatgcccgc cgtcgccttc gaacgctggt ataaacgcta
    63481 tctgaagcgc gcataagtag atgaatatat atacttagcg ctatatcccg tcattatata
    63541 tcgtattcct ggatgataat tagccgttgc tgttaaacac ttctctttta gcggcaattt
    63601 ttgcattatt cattttccac ttttgctata aggatgtcct tgatgtctga atttgatgcc
    63661 cagcgcgttg ccgaacgtat tgatattgtg ttagatatcc ttgtggcagg cgattaccac
    63721 tctgcgatcc ataatctcga gatacttaaa gcagaactct tgcgccaggt tgctgaatct
    63781 acgccagaca tcccgaaggc accgtgggag atttaacaat cgtgccttca ggttttccta
    63841 tttgaggatg cccggcgcgt cagtattttt ttatttagta ttataacgtt ataagaatta
    63901 cagcgatgaa ttcccgacaa caaactattc tacagatggt cattgaccag ggtcaggtta
    63961 gcgtaaccga tctggcaaaa gccactggag tttctgaagt taccattcgc caggatctca
    64021 acaccctcga aaaactgagt tacctccgcc gtgcacatgg ctttgcagtt tcgcttgata
    64081 gtgatgacgt cgaaacccgt atgatgagca actatacgct gaagcgtgaa ctcgccgagt
    64141 ttgccgcgtc actggttcaa ccgggcgaaa ccatctttat cgaaaatggt agcagcaatg
    64201 ccctgctcgc ccggactctg ggcgaacaga agaaaaatgt cactatcatc acagtcagca
    64261 gctacattgc gcatttgctg aaagacgcgc cttgtgaagt cattttgctc ggtggcgtgt
    64321 accagaaaaa aagcgaaagt atggttggcc ctttgacacg ccagtgcatc cagcaggtac
    64381 atttcagcaa agcatttatt ggtattgatg gctggcaacc tgaaactgga ttcaccggtc
    64441 gcgatatgat gcgtaccgat gtggtcaatg ccgtgctgga aaaagagtgc gaagcgatag
    64501 tcctgactga cagctcgaaa tttggtgctg tacattcata ctccatcggt cccgttgagc
    64561 gattcaatcg cgtgattacc gattcgaaaa tacgcgccag cgatctgatg catcttgagc
    64621 acagtaaact caccgttcac gtcgttgaca tttaaccatt tcccttctct acggatgatt
    64681 tgcagtttgg caaatcatcc gctctaagat gattcctggt tgataattaa gactatttac
    64741 ctgttattaa cactctcaag atataaaatt attatcagcg atataacagg aagtcattat
    64801 cacctgcgtg atataaccct gcgcgcgagc agatttcacg gaataatttc accaggctta
    64861 ttcttagcta ttatagttat agagagctta cttccgtgaa tcataaattc aggagagagt
    64921 attatgtttg taacgagcaa aaaaatgacc gcggctgttc tggcaattac tttggcaatg
    64981 tctctgagtg cctgttctaa ctggtctaaa cgggaccgca acaccgcaat cggcgcgggt
    65041 gcaggggcat taggcggtgc agtactgacc gatggcagta cgttgggtac attaggtggt
    65101 gcagctgtcg gtggtgttat tggtcaccag gtcggtaaat aatcgtaaat aaatgtcgat
    65161 tatcgactcg caattataat acgcttgatt ctgttcagta cgaaataact gaataatacg
    65221 cacagtaaaa tattcgaaag ccacggatat atccgtggct ttttatgtta accgcctgcg
    65281 agttttacct tcatcccttt cgcttcaagt agtgatttta ataaatcacg cttatcgccc
    65341 tggatttcaa taactccatc tttaactgct ccgccgcagc cgcatttttt cttcagttcc
    65401 gctgcaagtt ttgtcagttc ggcatcatcg agatcgacac cggtaatcag gcaaacgccc
    65461 ttacctttac gtccactggt ctgacgctga atacgcacca caccgtcgcc tttagggcgt
    65521 acaggggccg ctttgggctc atcaatacgt ccggtttccg ttgagtagac cagacggctg
    65581 ttggaatcac tcatcatgca ctccgctgta aagaggcgtt gatcgctttc agcgtctgcg
    65641 ctggatctac cgattgcgtt accgggcgac caatcaccat ataatcaaca ccagccgaca
    65701 acgcctgttc tggcgtcata atgcggcgct ggtcaccagc ttcactcccc tgcggacgaa
    65761 tgcccggcgt aaccagtttg aactcctgac cgaatacctg tttaaagcgc acagcttcct
    65821 gagcagaaca caccacacca tcaaggctac atttttgcgt cagtgccgcc agacgttctg
    65881 cataatctgc aggtgacagt gtcatgccaa gatcgaccag gtcgctggct tccatgctgg
    65941 tcaacactgt cacagcaatc aaaagcggtg catctttgcc aaacggaacc agtgcctcac
    66001 gcgctgcggt catcatacgc gccccaccag aggcatgaac attcaccatc cacacgccta
    66061 agtcagctgc agcagcgaca gcgtgcgctg cagtgttagg aatatcgtgg aatttcaggt
    66121 caagaaagat atcaaaacca cgctgttgaa gttcgcgcac aaactgtggc ccaaacaatg
    66181 taaacatctc tttgccgacc ttcagacgac aatcgcgtgg gtcgatcttg tcgacaaagg
    66241 acagcgcgtc atcacgatta tgataatcaa gggcaacaac cacaggagaa ttcgtaacag
    66301 cgcgggaaga agatgaagca gttaacgtca tgaccagacc ttcttgatga tgggcaccgg
    66361 aagtggcgcg aaacaggtaa acggcgagca ttctacccgt ggaaataaaa aattaacagt
    66421 tgcgattttt cccctggcag gctttttctg ctccgtcgtt gccggagcac aatggaacat
    66481 caataactag tatgttgtaa ctaaagtcgg atttttttaa aaattacagg ccatcaagac
    66541 cgcgaatcgg tttaatggtt gaccaggccc gacaagacgg acaatgccag tagagggtgt
    66601 atgcggtaaa accacatttc tggcagcgat aacgaggctt actacgtacc ttttcgccaa
    66661 ccatgtcacg cagcaccatc aggctctctt tggcacgccc ttcttccgct tcatttaagt
    66721 ggtaatccat taacttatgg aacacacgca tggtcggatg acgctgaagc tggcgcgtaa
    66781 tatagatctg tgcagcctca ctaccgtcgc gcgcttcgat gatatccgca agcatcaatt
    66841 cagcatcggc accggtgttc tcttccaccg cgcgttgcag gaattctgcc cattcggcag
    66901 ttttacccaa ctgctgatag caggtttgca acatttccag cgtttcgctg accagttctc
    66961 tgtcctggga tatcacgcgt tgcaaacttt cgacggcttt ggcgtattct cctttcgcca
    67021 taaacacgcg tcccatcatg atggatacgc gggcgctgtt tttatctgcc gccgcccctt
    67081 tttttagcaa ggtcatggca cgatcgagat cgtcgctggc catatgctgc agggctaact
    67141 cacagtagaa atgggcaatt tcgacgcgct gtttatcttt acccagcttc accaggcgtt
    67201 cggcaacatc aattgctttc tgccactcac tggtagcctg gtagatttgt agcaactgtt
    67261 gcagagcgcc aatgcggaag tcagtttcat cggtcagctg attgaacatg tcttccgcgc
    67321 ggtcgtataa cccggcggcc atgtaatcac gccctagttg ttgaatcgcc aacagacgct
    67381 gttcataggt cagcgaggcg ctttccatta gggtctgatg gatgcgaata gcgcgatcaa
    67441 cttcgccacg cgaacggaac aggtttccga gcgtaaggtg ggcttcaacg gtgcctgtat
    67501 cctctttaag catatcgaga aacaggtcta ccgctttatc ctgttgatta ctaagcagga
    67561 agttaacccc cgctacgtaa tcacgcgaca agcggttggc ttcatcttgc ttattttgtt
    67621 gcgcacttct gcggcccata taccagccat aggcagcggc tacaggcaaa agcagaaaca
    67681 acaactccag catagaaagt tattccttcg ccgctgacga gtgcggcact acagccacgt
    67741 cagtcgcggg tgaaagctgg ttttccagtc gctttatttt acgttcagcg cgcgccaggg
    67801 aaacacgaac tcgcagccag aacaggccac aaatcaacca accgatagca aaccccgcag
    67861 caaacaatac cgccagcaat gtggaaatac ggtactcccc ttgcgctaac agataattaa
    67921 acgtcacctg ttgatcgttc tgcgcaccca acgtgaccga aatcacgaag atcgctaaca
    67981 ccagtaagaa aatgagtaaa tatttcacat tacttcccgt tatgtggtta gagcgaataa
    68041 attatggtca acttaccgca atagcccttt aaagtaccat tttcgcgccg tctgcgaaat
    68101 ggcatccacg cgcattgttg atgatttaag ggctaaattc ggaaaatcaa ccagcgttaa
    68161 ctttcttgtt ctcgttgcgc tatttcgcga ttttcttccg caggtggtgt taatggccca
    68221 caaattcgtt gcgcaagcca cgttgccacc gccaccagcg cccacgaaat caacgtagct
    68281 actaccagat cgcgtggcca atgcatcccg agcagcaggc ggcttcccat gactcccgtt
    68341 gcccagacca gcaagatagc aatggttaac gttcgccgac gcggccacag caaaccaacg
    68401 gccagcagtg cccaactggc agcaaacatc gtgtgaccgg aaggaaaggc aaaccccgtc
    68461 tctttctgcc agtgtgaacg caaatattgt gggatatttt tctcttcagc caactgttct
    68521 ttcactaaat ttccgcgttc tgctcgcttt aaagtgtaga actcatcaac cggaatatga
    68581 tgtgtttttt ccagccagat aacaaaaggt cgtggttcct ggactttgtc tttgatccag
    68641 gatttaacgc cttgtcccac aaggattgcg gccgccagaa tggcaaataa tacaaaggca
    68701 gccttaatgc gaaaacgcag acaccagaga aaccagccga ataaaatcaa atgtgtaatg
    68761 acgccccagg gctgggtgac agtttcagta acccaaaaag ccgcttttag tagccaactt
    68821 tgttctccag gttgccaacg ccagccagaa atccatacgg ctactggcat gacaagcaat
    68881 agtgcagctc ccactgcggt acgtctggca atcgaacgca tggcctctcc tttttgataa
    68941 gtcccacaat cataactgaa aacgccagtt ccaggaaaaa ttgacagatt tgtgccattc
    69001 cgtgaacgat cgacgcgtcg tgattaggtg aaccccttct cgttatggca aaataagcca
    69061 atacagaacc agcattatct ggagaatttc atgcagctta aacgtgtggc agaagccaaa
    69121 ctgccaaccc catggggcga tttcctgatg gtgggatttg aagaactggc aaccggacac
    69181 gatcatgtcg cgctagtcta tggcgatatt tccgggcata ccccggtact tgcgcgcgtc
    69241 cattccgaat gtctgaccgg tgacgccctg ttcagcttgc gctgcgattg tggcttccag
    69301 ctcgaagcgg cattgacgca aattgccgag gaaggccgtg gtattttgct gtatcaccgt
    69361 caggaaggtc gtaacattgg tctgctgaat aaaatccgcg cttacgcact gcaggatcaa
    69421 ggttacgata ccgtagaggc taaccaccag ttaggcttcg ccgctgatga gcgcgacttc
    69481 actctttgcg ctgatatgtt caaactcctt ggcgtcaatg aagtccgctt gttaaccaat
    69541 aacccgaaaa aagtcgaaat tctgaccgaa gcagggatta atattgttga acgcgtacca
    69601 ttgattgtag gtcgtaaccc caataacgaa cattatctcg atactaaagc cgagaaaatg
    69661 ggccatttgc tgaacaaata accctcttgc attgtgtaat tagtttgctt gccggaagca
    69721 aaataaccgg caagcaaata cttgttactt caacatatta cgaatgacat aatgcaaaat
    69781 gccgtcgttc tggtagtagg tcaactccgt cgcggtgtcg atacgacaac ggcagggtac
    69841 gacctcctgg ctaccatccg cgcgcgtaag cgtcaccgga accgtcgcac cgggttgtag
    69901 attttgcaga tcgccaatat caatcttctc ttccccggtt agtcctaacg ttttacgcgt
    69961 tacgccttgc ggaaattcca gcggcaggat gcccatgcca attaaattcg aacggtgaat
    70021 tcgttcaaac gattcggcaa tcaccacacg aattccaagc agacgaggac cttttgccgc
    70081 ccagtcacgg cttgagcctg atccatactc tttcccggca atcaccgcca gcggcgtttg
    70141 ttcctgctta tagcgcatcg cagcatcata aatagagact acgtcgctgt caggtaaatg
    70201 ccgcgtcatc cccccttcaa cgccaggcac catttcatta cggatgcgaa tattggcgaa
    70261 ggtgccgcgc atcatcactt catggttacc acgccgcgaa ccgtaggagt taaagtcttt
    70321 tcgctcaaca ccccgacctt gtagatatcg acccgctggg ctgtcgggct taatactgcc
    70381 cgccggagag atatggtcag tggtgactga atcccccagc attgcgagga tccgcgcacc
    70441 gtgaatatct tccactggtg ctggtgttgc ctgcatttca tcaaagaaag gcgataagcg
    70501 aatataggtt gagtcctcct gccaaccgta ggtatcggat cgtgtgacgt taatttcctt
    70561 ccactctgct gtgccttcaa aaacttctgc gtactctttg cggaacattt ctgtggagac
    70621 ttgttctacc gcacgggcaa tttcttgtgc cgatggccag atatctttca gataaaccgg
    70681 gtcgccttta cgatcatgac caataggctc agcagccagg ttgatattca tatttcccgc
    70741 cagcgcatag gcaaccacca gcggcggcga ggccagccag ttagttttaa ccagcggatg
    70801 gatacggcct tcaaagttac ggttgccgga cagcaccgca ccgacggtta aatcgccttt
    70861 tttgattgcc gtttcgatag gatcgggcag cggcccagag ttaccaatac aggtggtaca
    70921 accgtatccc acaaggttaa accccagttc gtcgagatac ggtgtcagtt tcgcttttgc
    70981 cagataatca gaaacgactt tcgaacccgg tgccagcgac gctttgaccc acggttgccg
    71041 cttgaggccc agagttacgg cttttttcgc cagcaagcct gcggccatca gcacacttgg
    71101 gttagaggtg ttggtgcacg aggttatcgc agcaatgacc acagcgccat caggtaactg
    71161 atactgatgt ccgttcataa cataatcgac cggctggcga tttttatgcg tggcattcac
    71221 ttccagttcg ttactggcgg caaatgcttt tggtacatcg ggcagtgcaa cgcgatcctg
    71281 tgggcgtttt ggccctgcca ggctcgcttc aacgtcattc atatccagtt ctaacgtact
    71341 ggtaaaaatc ggttcatcgc ccgggttacg ccacatgccc tgcgctttgg catatttttc
    71401 gaccaactca acctgatctt cgctgcgccc gcttaaacgc atgtaatcga gggttacagc
    71461 atcgattggg aagaagccac aggtggcacc atattctggc gacatattgg caatggtggc
    71521 gcgatccgcc agcggtagtg aatccagacc atcaccataa aattcgacga atttccccac
    71581 cacgccatgt ttgcgcagca tttgggtaac agtgagaacc aggtctgtgg cggtaatacc
    71641 ttcacgtaat ttaccggtaa gtttaaagcc cactacgtcc gggataagca tggaaaccgg
    71701 ctggccta
//
LOCUS       DAZFXT010000010        71510 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.010, whole genome shotgun sequence.
ACCESSION   DAZFXT010000010 DAZFXT010000000
VERSION     DAZFXT010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 71510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71510
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.010"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            346..930
                     /gene="yadN"
                     /locus_tag="ACHKW0_000724"
     CDS             346..930
                     /gene="yadN"
                     /locus_tag="ACHKW0_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7473608.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            1035..1775
                     /gene="yadV"
                     /locus_tag="ACHKW0_000725"
     CDS             1035..1775
                     /gene="yadV"
                     /locus_tag="ACHKW0_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO7473609.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1810..4410
                     /gene="htrE"
                     /locus_tag="ACHKW0_000726"
     CDS             1810..4410
                     /gene="htrE"
                     /locus_tag="ACHKW0_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HFO7473610.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAEPVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGLLGLGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWN
                     KLFEETSTSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVT
                     VSINQPLKFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWN
                     EDGDTDDSVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNA
                     RVSYSVNTGYTMNKASKDLSYVGGYASYESPWGSLAGSVSANSDNSRQVSLSTDGGFV
                     LHSGGLTFSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRI
                     ALDINDLENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADI
                     YDEQGNVIGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSI
                     ILNGIRCQIQ"
     gene            4427..4996
                     /gene="yadM"
                     /locus_tag="ACHKW0_000727"
     CDS             4427..4996
                     /gene="yadM"
                     /locus_tag="ACHKW0_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7473611.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            5011..5613
                     /gene="yadL"
                     /locus_tag="ACHKW0_000728"
     CDS             5011..5613
                     /gene="yadL"
                     /locus_tag="ACHKW0_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HFO7473612.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTAADKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            5640..6236
                     /locus_tag="ACHKW0_000729"
     CDS             5640..6236
                     /locus_tag="ACHKW0_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO7473613.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            6220..7563
                     /locus_tag="ACHKW0_000730"
     CDS             6220..7563
                     /locus_tag="ACHKW0_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HFO7473614.1"
                     /translation="MCTTNNRSKQITATVTAVDGGVIMKKFFRHFLFLILCLSCYTAS
                     AGTDDNVGYIVGNSYGVGPSDQKWRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTG
                     VKLQWSQLDTASGGGFLYCNRSDSTSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYY
                     TLLISNMWSAYGTVTNVSSPGIYIGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAV
                     GGIYQTLTIEFYTDTNFDPTVTQQIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFD
                     LLNVKLTNPTCFTAMLSGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNI
                     ETKLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKD
                     YDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            7676..8470
                     /gene="panB"
                     /locus_tag="ACHKW0_000731"
     CDS             7676..8470
                     /gene="panB"
                     /locus_tag="ACHKW0_000731"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HFO7473615.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            8482..9333
                     /gene="panC"
                     /locus_tag="ACHKW0_000732"
     CDS             8482..9333
                     /gene="panC"
                     /locus_tag="ACHKW0_000732"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HFO7473616.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(9407..10309)
                     /gene="rpnC"
                     /locus_tag="ACHKW0_000733"
     CDS             complement(9407..10309)
                     /gene="rpnC"
                     /locus_tag="ACHKW0_000733"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HFO7473617.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            10583..10963
                     /gene="panD"
                     /locus_tag="ACHKW0_000734"
     CDS             10583..10963
                     /gene="panD"
                     /locus_tag="ACHKW0_000734"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HFO7473618.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(10967..12196)
                     /gene="yadE"
                     /locus_tag="ACHKW0_000735"
     CDS             complement(10967..12196)
                     /gene="yadE"
                     /locus_tag="ACHKW0_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7473619.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(12260..12700)
                     /gene="yadI"
                     /locus_tag="ACHKW0_000736"
     CDS             complement(12260..12700)
                     /gene="yadI"
                     /locus_tag="ACHKW0_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7473620.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(12805..13575)
                     /gene="yadH"
                     /locus_tag="ACHKW0_000737"
     CDS             complement(12805..13575)
                     /gene="yadH"
                     /locus_tag="ACHKW0_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7473621.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(13572..14498)
                     /gene="yadG"
                     /locus_tag="ACHKW0_000738"
     CDS             complement(13572..14498)
                     /gene="yadG"
                     /locus_tag="ACHKW0_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7473622.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            14607..15269
                     /gene="can"
                     /locus_tag="ACHKW0_000739"
     CDS             14607..15269
                     /gene="can"
                     /locus_tag="ACHKW0_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HFO7473623.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(15310..15846)
                     /gene="hpt"
                     /locus_tag="ACHKW0_000740"
     CDS             complement(15310..15846)
                     /gene="hpt"
                     /locus_tag="ACHKW0_000740"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HFO7473624.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            16052..18442
                     /gene="gcd"
                     /locus_tag="ACHKW0_000741"
     CDS             16052..18442
                     /gene="gcd"
                     /locus_tag="ACHKW0_000741"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HFO7473625.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(18489..20039)
                     /gene="cueO"
                     /locus_tag="ACHKW0_000742"
     CDS             complement(18489..20039)
                     /gene="cueO"
                     /locus_tag="ACHKW0_000742"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HFO7473626.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            20205..20552
                     /gene="yacC"
                     /locus_tag="ACHKW0_000743"
     CDS             20205..20552
                     /gene="yacC"
                     /locus_tag="ACHKW0_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HFO7473627.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            20658..21524
                     /gene="speE"
                     /locus_tag="ACHKW0_000744"
     CDS             20658..21524
                     /gene="speE"
                     /locus_tag="ACHKW0_000744"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HFO7473628.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            21540..22334
                     /gene="speD"
                     /locus_tag="ACHKW0_000745"
     CDS             21540..22334
                     /gene="speD"
                     /locus_tag="ACHKW0_000745"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HFO7473629.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(22372..22734)
                     /gene="yacL"
                     /locus_tag="ACHKW0_000746"
     CDS             complement(22372..22734)
                     /gene="yacL"
                     /locus_tag="ACHKW0_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YacL"
                     /protein_id="HFO7473630.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVIVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(22910..25507)
                     /gene="acnB"
                     /locus_tag="ACHKW0_000747"
     CDS             complement(22910..25507)
                     /gene="acnB"
                     /locus_tag="ACHKW0_000747"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HFO7473631.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAVVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            25862..27619
                     /gene="yacH"
                     /locus_tag="ACHKW0_000748"
     CDS             25862..27619
                     /gene="yacH"
                     /locus_tag="ACHKW0_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HFO7473632.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTASPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAENYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQIQQRRDAARERIQSASPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(27690..29114)
                     /gene="lpdA"
                     /locus_tag="ACHKW0_000749"
     CDS             complement(27690..29114)
                     /gene="lpdA"
                     /locus_tag="ACHKW0_000749"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HFO7473633.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(29439..31331)
                     /gene="aceF"
                     /locus_tag="ACHKW0_000750"
     CDS             complement(29439..31331)
                     /gene="aceF"
                     /locus_tag="ACHKW0_000750"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004742 - dihydrolipoyllysine-residue
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HFO7473634.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKETAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKIAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(31346..34009)
                     /gene="aceE"
                     /locus_tag="ACHKW0_000751"
     CDS             complement(31346..34009)
                     /gene="aceE"
                     /locus_tag="ACHKW0_000751"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HFO7473635.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(34170..34934)
                     /gene="pdhR"
                     /locus_tag="ACHKW0_000752"
     CDS             complement(34170..34934)
                     /gene="pdhR"
                     /locus_tag="ACHKW0_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HFO7473636.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            35475..36848
                     /gene="aroP"
                     /locus_tag="ACHKW0_000753"
     CDS             35475..36848
                     /gene="aroP"
                     /locus_tag="ACHKW0_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HFO7473637.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(36891..37745)
                     /gene="ampE"
                     /locus_tag="ACHKW0_000754"
     CDS             complement(36891..37745)
                     /gene="ampE"
                     /locus_tag="ACHKW0_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HFO7473638.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(37742..38293)
                     /gene="ampD"
                     /locus_tag="ACHKW0_000755"
     CDS             complement(37742..38293)
                     /gene="ampD"
                     /locus_tag="ACHKW0_000755"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HFO7473639.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            38381..39274
                     /gene="nadC"
                     /locus_tag="ACHKW0_000756"
     CDS             38381..39274
                     /gene="nadC"
                     /locus_tag="ACHKW0_000756"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HFO7473640.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            39477..39917
                     /gene="ppdD"
                     /locus_tag="ACHKW0_000757"
     CDS             39477..39917
                     /gene="ppdD"
                     /locus_tag="ACHKW0_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HFO7473641.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALHQACEDVFRFDDAN"
     gene            39927..41312
                     /gene="gspE"
                     /locus_tag="ACHKW0_000758"
     CDS             39927..41312
                     /gene="gspE"
                     /locus_tag="ACHKW0_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HFO7473642.1"
                     /translation="MNIPQLTALCLRYHGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLTRTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVSQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPDNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMRTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            41302..42504
                     /gene="hofC"
                     /locus_tag="ACHKW0_000759"
     CDS             41302..42504
                     /gene="hofC"
                     /locus_tag="ACHKW0_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HFO7473643.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWSW
                     LLVLFGFLLAIANKLLMRRPTWLIVRQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNTGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(42539..43582)
                     /gene="guaC"
                     /locus_tag="ACHKW0_000760"
     CDS             complement(42539..43582)
                     /gene="guaC"
                     /locus_tag="ACHKW0_000760"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902560 - GMP reductase complex [Evidence
                     IEA];
                     GO_function: GO:0003920 - GMP reductase activity [Evidence
                     IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HFO7473644.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(43738..43782)
                     /locus_tag="ACHKW0_000761"
     CDS             complement(43738..43782)
                     /locus_tag="ACHKW0_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473645.1"
                     /translation="MIIKTLKMSARKRN"
     gene            43807..44427
                     /gene="coaE"
                     /locus_tag="ACHKW0_000762"
     CDS             43807..44427
                     /gene="coaE"
                     /locus_tag="ACHKW0_000762"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HFO7473646.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            44427..45170
                     /gene="zapD"
                     /locus_tag="ACHKW0_000763"
     CDS             44427..45170
                     /gene="zapD"
                     /locus_tag="ACHKW0_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HFO7473647.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            45180..45377
                     /gene="yacG"
                     /locus_tag="ACHKW0_000764"
     CDS             45180..45377
                     /gene="yacG"
                     /locus_tag="ACHKW0_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HFO7473648.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(45477..45866)
                     /gene="mutT"
                     /locus_tag="ACHKW0_000765"
     CDS             complement(45477..45866)
                     /gene="mutT"
                     /locus_tag="ACHKW0_000765"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HFO7473649.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(45926..48631)
                     /gene="secA"
                     /locus_tag="ACHKW0_000766"
     CDS             complement(45926..48631)
                     /gene="secA"
                     /locus_tag="ACHKW0_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HFO7473650.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTVEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(48693..49205)
                     /gene="secM"
                     /locus_tag="ACHKW0_000767"
     CDS             complement(48693..49205)
                     /gene="secM"
                     /locus_tag="ACHKW0_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secA translation cis-regulator SecM"
                     /protein_id="HFO7473651.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(49436..50353)
                     /gene="lpxC"
                     /locus_tag="ACHKW0_000768"
     CDS             complement(49436..50353)
                     /gene="lpxC"
                     /locus_tag="ACHKW0_000768"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HFO7473652.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(50454..51605)
                     /gene="ftsZ"
                     /locus_tag="ACHKW0_000769"
     CDS             complement(50454..51605)
                     /gene="ftsZ"
                     /locus_tag="ACHKW0_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HFO7473653.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(51666..52928)
                     /gene="ftsA"
                     /locus_tag="ACHKW0_000770"
     CDS             complement(51666..52928)
                     /gene="ftsA"
                     /locus_tag="ACHKW0_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HFO7473654.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(52925..53755)
                     /gene="ftsQ"
                     /locus_tag="ACHKW0_000771"
     CDS             complement(52925..53755)
                     /gene="ftsQ"
                     /locus_tag="ACHKW0_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0090529 - cell septum assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HFO7473655.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(53757..54677)
                     /gene="ddlB"
                     /locus_tag="ACHKW0_000772"
     CDS             complement(53757..54677)
                     /gene="ddlB"
                     /locus_tag="ACHKW0_000772"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7473656.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(54670..56145)
                     /gene="murC"
                     /locus_tag="ACHKW0_000773"
     CDS             complement(54670..56145)
                     /gene="murC"
                     /locus_tag="ACHKW0_000773"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HFO7473657.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(56199..57266)
                     /gene="murG"
                     /locus_tag="ACHKW0_000774"
     CDS             complement(56199..57266)
                     /gene="murG"
                     /locus_tag="ACHKW0_000774"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HFO7473658.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(57263..58507)
                     /gene="ftsW"
                     /locus_tag="ACHKW0_000775"
     CDS             complement(57263..58507)
                     /gene="ftsW"
                     /locus_tag="ACHKW0_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HFO7473659.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(58507..59823)
                     /gene="murD"
                     /locus_tag="ACHKW0_000776"
     CDS             complement(58507..59823)
                     /gene="murD"
                     /locus_tag="ACHKW0_000776"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HFO7473660.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(59826..60908)
                     /gene="mraY"
                     /locus_tag="ACHKW0_000777"
     CDS             complement(59826..60908)
                     /gene="mraY"
                     /locus_tag="ACHKW0_000777"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HFO7473661.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGIAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(60902..62260)
                     /gene="murF"
                     /locus_tag="ACHKW0_000778"
     CDS             complement(60902..62260)
                     /gene="murF"
                     /locus_tag="ACHKW0_000778"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HFO7473662.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(62257..63744)
                     /gene="murE"
                     /locus_tag="ACHKW0_000779"
     CDS             complement(62257..63744)
                     /gene="murE"
                     /locus_tag="ACHKW0_000779"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HFO7473663.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKGEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIVNADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(63731..65497)
                     /gene="ftsI"
                     /locus_tag="ACHKW0_000780"
     CDS             complement(63731..65497)
                     /gene="ftsI"
                     /locus_tag="ACHKW0_000780"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HFO7473664.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(65513..65878)
                     /gene="ftsL"
                     /locus_tag="ACHKW0_000781"
     CDS             complement(65513..65878)
                     /gene="ftsL"
                     /locus_tag="ACHKW0_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HFO7473665.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(65875..66816)
                     /gene="rsmH"
                     /locus_tag="ACHKW0_000782"
     CDS             complement(65875..66816)
                     /gene="rsmH"
                     /locus_tag="ACHKW0_000782"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HFO7473666.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(66818..67276)
                     /gene="mraZ"
                     /locus_tag="ACHKW0_000783"
     CDS             complement(66818..67276)
                     /gene="mraZ"
                     /locus_tag="ACHKW0_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HFO7473667.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(67878..68882)
                     /gene="cra"
                     /locus_tag="ACHKW0_000784"
     CDS             complement(67878..68882)
                     /gene="cra"
                     /locus_tag="ACHKW0_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009750 - response to fructose [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HFO7473668.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(68964..69050)
                     /locus_tag="ACHKW0_000785"
     CDS             complement(68964..69050)
                     /locus_tag="ACHKW0_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HFO7473669.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(69062..69553)
                     /gene="ilvN"
                     /locus_tag="ACHKW0_000786"
     CDS             complement(69062..69553)
                     /gene="ilvN"
                     /locus_tag="ACHKW0_000786"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7473670.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(69556..71280)
                     /gene="ilvI"
                     /locus_tag="ACHKW0_000787"
     CDS             complement(69556..71280)
                     /gene="ilvI"
                     /locus_tag="ACHKW0_000787"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HFO7473671.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVCNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            71399..71443
                     /locus_tag="ACHKW0_000788"
     CDS             71399..71443
                     /locus_tag="ACHKW0_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473672.1"
                     /translation="MKRNDKILHYEGLN"
BASE COUNT        17549 a        19265 c        17350 g        17346 t
ORIGIN      
        1 attttctgaa aatcatacat cctctctttc cttaataaaa attttagttt aaacattttt
       61 gcagaaacat aatataaatt cccgcagata aatatcaatc ccatcgccat accctaacca
      121 attgcacttg tgaatttatt tttgcttaat tttgaattaa gaaaatttaa tttgttcaaa
      181 agcccttaaa gtgcacaata ggcgtcctaa tgaacaatgg ttgtaaattt tggtaacaaa
      241 tatcattgct cattaatatc tagcatctgc ttataacatt tgtgtcatga atagtcattc
      301 ttcaaaccag tagtctgact aatggaattt tattcaagga tttttatgtc taaaaaatta
      361 ggttttgccc tgtctggtct tatgctggca atggttgcag gtactgcttc tgctgatatg
      421 gatggtggtc agttaaatat cagtggtctg gttgttgata acacctgtga aacccgcgtt
      481 gacggcggta ataaagatgg tctgatcctg ctgcaaactg caactgttgg tgaaatcgat
      541 gctggcgtgc tgaatgacac cgttggcgct aaagccaaac ctttcagcat caccgttgac
      601 tgcagcaaag caaatccgaa tccaggtagt acagctaaaa tgaccttcgg ttctgttttc
      661 tttggtaata gcaaaggaac gctgaacaac gacatgtcta ttaacaaccc gtctgacggc
      721 gtgaatatcg ctctgcacaa tattgatggt tccactatca aacaggttca aattaacaac
      781 cctggcgatg tgtatactaa agccctggat gcaacgacaa aatctgctgt ttatgatttt
      841 aaagcgtctt acgttcgtgc cgtcgcagac caaacagcaa ctgctggtta tgtaaaaact
      901 aacactgcat acaccattac ttatcagtaa gtattcacca attaaattga ttgaatgaat
      961 atacagggaa tagtgttaat aatttctatt tttatattat tccctgtttt aattaactct
     1021 gtcagggatg gtttatgttt tttaacacca aacatacaac agctttatgc ttcgtaacct
     1081 gtatggcttt tagttcatcg tctattgcgg acattgtcat ttcgggtact cgcgtaatat
     1141 ataaaagcga tcaaaaaagt gtcaacgtac gtctggaaaa taaagggaat aacccgttgc
     1201 ttgtccagag ttggttagat actggcgatg acaacgctga gcctggcagt attacagtcc
     1261 cttttactgc cacgccgcca gtatcgcgta ttgatgccaa acgtgggcaa acaatcaaat
     1321 taatgtacac agccagcacc tcactgccta aagacagaga gagcgtgttc tggtttaacg
     1381 tactggaagt tccaccaaaa ccagatgcag aaaaggtcgc gaatcaaagt ctgctgcaac
     1441 tggcatttcg cacacgtata aaacttttct atcgcccgga tggattgaag ggaaatccct
     1501 ctgaagcccc gttagccctg aagtggttct ggtcaggttc agaaggtaag gcgtcattgc
     1561 gagtgaccaa tccaacccct tactacgtct cttttagcag tggtgattta gaagctagcg
     1621 gtaaacgcta tccgattgat gtgaaaatga ttgcaccatt tagtgatgag gtcatgaaag
     1681 tcactggcct taatggcaaa gcaagttctg caaaagtaca tttttacgcc attaatgatt
     1741 ttggtggcgc cattgaaggt aatgccagtc tgtaatcagg caaggatata attccgcagg
     1801 aagcataacg tgactataaa accaactaaa aattatcata atcatctgac ccgtatcgct
     1861 acgttttgcg cgctgctgta ttgcaatagt gctttctgtg ctgaacctgt tgaatatgac
     1921 cataccttcc tgatggggca gaatgcctca aatattgatc tcagccggta tagtgaaggt
     1981 aaccctgcta taccgggtat gtacgacgtc agtgtttatg tcaacgatca acctattatt
     2041 aaccaaagca ttacatttat tgaaattgaa ggaaaaaaaa acgcacaagc ctgtatcaca
     2101 cttaagaatt tattgcagtt tcatataaat tctcccgata taaataacga aaaagccgtt
     2161 ctgcttgcca gggatgaaac actcggcaat tgccttaatt tgacggaaat tatccctcag
     2221 gcttctgttc gttatgacgt taacgagcaa cgtctggata tagacgttcc tcaggcatgg
     2281 gtaatgaaaa attaccaaaa ctatgttgat ccatcgttat gggaaaatgg cattaatgcg
     2341 gccatgttgt catacaacct caacggatat catagtgaaa ctcctggtcg aagaaatgac
     2401 agcatttatg ctgcttttaa cggtgggatg aatttaggtg catggcgact gcgtgcctca
     2461 ggcaactaca actggatgac cgattctggc agtaattatg attttaagaa tcggtatata
     2521 cagcgtgata tcgcctcgct gcgttctcaa ctcattcttg gtgagtctta tacaacgggc
     2581 gaaacctttg attctgtcag tatccgcggc attcgtttat acagcgacag ccgcatgttg
     2641 cctccgacat tagcgagttt tgcgcctatc attcatggcg ttgctaatac caacgccaaa
     2701 gtcactatta cgcaaggtgg ctataagatt tatgaaacga ctgtgccgcc aggcgctttc
     2761 gtcattgatg atctgagtcc gtcaggttac ggcagcgatc ttattgttac tgtcgaagaa
     2821 tccgatggct caaagcggac attctcgcaa cctttttcat cggtcgttca aatgttacgc
     2881 cctggcgttg gacgttggga tattagcggc ggccaggtct taaaagatga tattcaggat
     2941 gagcctaatt tatttcaggc cagctactac tacggtctga ataactatct aacgggttat
     3001 actggtattc agattaccga taataactat accgctggtt tgttaggtct tggtctgaat
     3061 acttcagtcg gtgcattttc tttcgatgtg actcactcca atgttcgtat accggatgat
     3121 aaaacatatc agggacaaag ttatcgtgtt tcctggaaca agttattcga agaaacaagt
     3181 acttcactga atatcgcggc ctatcgctat tcgacacaga attaccttgg tcttaatgat
     3241 gcactaactc taattgatga agtgaaacat cccgaacaag atcttgaacc gaaatccatg
     3301 cgtaattact cgcgcatgaa aaatcaggtt acggtcagta ttaaccaacc gctaaaattt
     3361 gagaaaaaag attacggttc attttatctt tccggaagtt ggtccgatta ctgggcttcc
     3421 ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg gggcagctac
     3481 agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag cgtttatctc
     3541 agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc aggtttccag
     3601 agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa cgtcagtagc
     3661 agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta tacgatgaat
     3721 aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc accgtgggga
     3781 agcctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc tctcagcacc
     3841 gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag ttttagcgac
     3901 tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat aaattatggc
     3961 aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc ttatcatgaa
     4021 aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa aagtaccagt
     4081 gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac cgtgcaaggg
     4141 caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt tgctgcagat
     4201 atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca agcatttgtt
     4261 cgtggtattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag taaacccgta
     4321 agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca atctattatt
     4381 ctgaatggaa ttcggtgtca gattcagtaa ctacaaggga cgtcaaatga taaaaataac
     4441 gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat ttgcaacgga
     4501 tattaatgta gagtttacag ccactgtcaa agcgacaacc tgtaacatca cacttactgg
     4561 taataacgtc acgaatgatg gcaataataa ctacacattg agaatcccta agatgggtct
     4621 ggataagatc gcgaataaaa cgacagaatc tcaggctgat tttaaactgg ttgctagtgg
     4681 gtgcagcagt ggcatcagtt ggattgatac cactctgacc ggaaatgcat catcaagctc
     4741 acctaagctt attataccgc agtctggtga ttcatcttcg acgacaagta atatcggtat
     4801 gggtttcaaa aaacggacta ctgatgatgc cactttcctt aaacctaaca gtgcggaaaa
     4861 gatacgctgg agcacagacg agatgcagcc cgataagggt cttgaaatga ccgttgcgct
     4921 acgtgaaaca gatgcagggc aaggcgtacc ggggaatttt cgtgcactgg caacgtttaa
     4981 tttcatctat caataagaag gataattaag atgacattta aaaatttacg ttatggatta
     5041 tccagcagtg tcgttttggc tgcctcattg ttcagcgtac cctcttatgc ggcaacagac
     5101 agcattgggc tgaccgtgat tactaccgta gaaataggta cttgtactgc tacattagta
     5161 aatgactctg atcaggacat ttctgttgtt gactttggcg atgtatatat ttctgaaatc
     5221 aatgccaaga ccaaagttaa aacattcaaa ctcaaattca aagactgtgc gggtatcccc
     5281 aataaaaaag cacaaataaa attaaccaag cgagccacat gcgaaggtac tgctaatgac
     5341 ggtgcggggt tcgcaaatgg atccacagcc gcagataaag caagtgccgt cgccgttgaa
     5401 gtctggagca ccgaaactcc ggcaacaggg agcgcaacac aatttagttg tgtgacacca
     5461 gcatctcaag aggtcactat ctccaatgca gccaacgcgg ttgtttatta tccgatgagt
     5521 gctcgactgg tcgtcgaaaa aaataaaacc gtaagcaatg tcactgcggg taaattttct
     5581 gcacctgcca catttacagt aacctataac taacagactg acagatccag gaactatata
     5641 tgcatcccac tcaacgtaag ctgatgaaga gaataattct gtttctgtca ctacagtttc
     5701 ccattaccta tccagccatc gctgggcagg atattgacct tgttgccaat gtaaaaaaca
     5761 gcacttgcaa aagcggaatc agtaaccagg gtaatattga tcttggcgtc gttggggtgg
     5821 gatatttttc agataatgtt actcctgaaa gctatcaacc aggtggaaaa gagttcactg
     5881 tcacagtatc agactgtgca ttacagggaa ctggcgatgt gcttaatcag ttacatattg
     5941 attttagagc ccttagcggt gtcatggctg caggttcaag gcaaatattt gccaatgaag
     6001 tcgcaaccgg ggctaaaaat gtcggtgtcg ttctcttttc cattcaggac ccgaccaata
     6061 tattcaatgt tattagttca gcaggaaatt ctcgttccgt gtatccagta atgacttctg
     6121 ccctgcataa ttcttcatgg aaattttatg ccagaatgca aaaaatcgat cccgcgctgg
     6181 atgtcatatc tggccaggta atgagtcata tattagttga tgtgtactac gaataataga
     6241 tcaaaacaaa taactgcgac tgttacagca gtagatggtg gagtgataat gaagaaattc
     6301 ttcagacatt ttcttttttt aattctgtgc ttgagttgtt ataccgcgag tgctggcact
     6361 gatgataacg taggatatat tgttgggaat agttacggcg ttggtccgtc agatcaaaaa
     6421 tggagagaaa cggggccgaa tggcgatgct actgtcatat tcagatatgc aacaagtaca
     6481 aataatttag ttttctacaa accgacgcag cttggaccta caggtgtaaa attacagtgg
     6541 agtcagttag ataccgcttc tggtggtggt tttctttatt gcaacagaag tgatagcaca
     6601 agtggtagcg caatgcgtat tgaaaatgca atggttgact caggtaaaat gtatggctcc
     6661 cataaattat ttaatacatc agttcctggt ttgtattaca cattattaat ttcaaacatg
     6721 tggtcagctt acggtaccgt aactaacgtt agttcacctg ggatatacat tggtgactct
     6781 gcagaacaat atttttcgtg gtataatcca agcgaagacg tgttatattg gagttgcaat
     6841 aatgcgaata gcacccgtaa atactgggct gtaggtggta tttatcagac ccttacaatt
     6901 gaattctata cagatacaaa ctttgatcca acagtaactc aacagatcaa attatccagc
     6961 tcatcaaatt atctgtattc atttaaagcc tatggcgcag gccaaggtat aaatgagcat
     7021 agttatttta tcaaaatcga tttcgatctg ctcaatgtca agttgactaa tcccacttgt
     7081 tttaccgcta tgcttagtgg aacttctgtg actggttcta cggtaaaaat gggtgaatat
     7141 agtgcagaac aaatcagaaa cggtgccaca ccggttcctt ttgatatttc acttcaaaac
     7201 tgcgttcgtg tgactaatat tgagacaaaa ttagtttcaa caaaggttgg tactgaaaac
     7261 gggcaactcc ttggtaatac tctcacaggt aatgacgcag caaaaggagt cggtgtactc
     7321 atagaaggtt tagcaactag taaaaatcct ctaatgacat tgaaacctaa tgattcaaat
     7381 tctgtttata aagattacga cccaagaggc aaagacgata caacaggagg agtttacccg
     7441 gatcaagata caggtataac ataccctctc catttccagg ccacgctaca acaggatgga
     7501 actataccaa tagaagctgg tgagtttaaa gccaccagta ctttccaggt aacctaccct
     7561 taataagtcc accgcaccgc catcatctgg cggtgcggta attgataaat cattgccccc
     7621 accaatgtga ctgataaaat gccaggcaac acgacatcat ttatcaggac acgttatgaa
     7681 accgaccacc attgcttcac ttcagaaatg taagcaggat aaaaagcgtt tcgcgaccat
     7741 caccgcttac gactacagct tcgcgaaact atttgctgat gaaggactta acgtcatgct
     7801 ggtaggcgat tcgctgggca tgacggttca gggccacgac tccacccttc ccgttaccgt
     7861 cgaggatatc gcctatcata ccgccgccgt acgtcgcggc gcgccaaact gcctgctgtt
     7921 ggctgacctg ccgtttatgg cgtatgccac gccggaacaa gcctttgaaa acgccgcaac
     7981 ggttatgcgt gccggtgcca atatggtcaa aattgagggc ggtgagtggc tggtcgaaac
     8041 cgtaaaaatg ctgaccgaac gtgccgttcc tgtatgtggt cacttaggtt taaccccaca
     8101 gtcagtgaat attttcggtg gctacaaagt tcaggggcgc ggcgatgaag cgggcgatca
     8161 actgctcagc gatgcattag ccttagaagc cgctggggca cagctgctgg tgctggaatg
     8221 cgtgccggtt gaactggcaa aacgtattac cgaagcactg gcgatcccgg ttattggcat
     8281 tggcgcaggc aacgtcactg acgggcagat cctcgtgatg cacgacgcct tcggcattac
     8341 cggcggtcac attcctaaat tcgctaaaaa tttcctcgcc gaaacgggcg acatccgcgc
     8401 ggctgtgcgg cagtatatgg ctgaagtgga gtccggcgtt tatccgggcg aagaacacag
     8461 tttccattaa ggagtcacgt tgtgttaatt atcgaaaccc tgccgctgct gcgtcagcaa
     8521 attcgccgcc tgcgtatgga aggcaagcgc gtggcgctgg tgcctactat gggtaatctg
     8581 cacgatggcc atatgaagct ggtcgacgaa gccaaagccc gcgccgatgt ggtcgtcgtc
     8641 agtattttcg ttaacccgat gcagttcgac cgcccggaag atctggcacg ttatccacgg
     8701 accttgcagg aggactgcga gaagctaaac aaacgtaaag tggatttagt tttcgcccct
     8761 tcggtaaaag agatctaccc gaacggtact gaaacccata cttacgttga cgttcccggg
     8821 ctttcgacca tgctggaagg cgcgagccgt ccggggcatt ttcgcggcgt ttcgactatc
     8881 gtcagcaagc tgtttaacct ggtccagccg gacatcgcct gcttcggtga aaaggacttt
     8941 cagcaactgg cgctgatccg caaaatggtt gccgatatgg gcttcgatat tgagattgtc
     9001 ggtgtgccaa ttatgcgcgc caaagacggt ctggcgctaa gttcccgtaa cggttatctg
     9061 acggcggaac aacgcaaaat tgcgcctggt ctgtacaaag ttttaagttc gattgctgac
     9121 aaattgcagg ctggggaacg ggatctcgat gaaattatta ccattgcggg gcaagaactg
     9181 aatgaaaaag gcttccgcgc cgatgatatt cagattcgcg atgccgacac attgctggaa
     9241 gtttctgaaa ccagcaaacg ggcagtaatt ctggtagccg cctggcttgg cgatgctcgc
     9301 ctgatcgaca acaaaatggt cgagctggcg taatacttaa ctggcgctac ggctgatggc
     9361 gccagttatt aatttacccc acgtcatctg tcataacaca ggtaacttaa ataaggttaa
     9421 ttaccttatc aatctcagca agagataaat ttgtcatctc tgcaacgtct tcccgagaca
     9481 ttcctttact tagaagacgc agggcgaatt cttgcctttc ctcctgtctt ccctgctgaa
     9541 tccccttctc aatccctttc tcttcaaacc actgcgccag cgtcatcata gactcccctc
     9601 ccgtttccct gtctctcagc acaccataaa acaaatccgc ttgttcagta tgaccgcgtt
     9661 gcagcatata gttttgcatg gcaactaact gacttccgct agtgtaccct tcgtcaatca
     9721 gcgtgaccag ttgctccagc aataacatta agtcgcgctg gcgaatatgt ttttgcagta
     9781 gttcgagaat cgcaatccgc cgatgttgca tgatttcgtc atccggtgtg atggtgatat
     9841 ccaccagcgg gaaaggactg ttatagacgc gtcgtgccag ctccggcgag taaaatatat
     9901 caaaccagca cattgagagc ggataaggtg tggcctcgcc ctgataaaac agtatcggca
     9961 ccaccagcgg cagcttatcg tgatccgcct ccagatgccg gtgcatggca gcaatagaat
    10021 aacgcatcat gcgaaaggcc attttcttat ccggcttgct ttggtgttca atcacaacat
    10081 gcagataacc gggattgccc tgcatttgca cggaatagag cacgtccgtg ctgtgtcctt
    10141 tcaggctctc ttcaatgaaa ctccccgact ctaaatgaag cgtgttgagg tcacaaagtt
    10201 ctcgtaattc cactggcaaa tgtatctcca gaaagtcgcg agccgtctcc gcatgcatta
    10261 aaaattgttt aaataccgcg tcatgcggtg tggtactcgg tgcatccatc ttccctgtct
    10321 tccgtgttgt cttgcgtgaa ggcagtatcc agtcaaggcg cgccgtcgtc agccgcctct
    10381 ttagtgttta ccgagcagcg ttcagagaaa tttcaattcg ttacaggcga tacatggcac
    10441 gcttcggcgc ggtacgcaaa ctgctgacgc aaatgccatc agatgagtag acactcatca
    10501 aaaatcgggc aatactgcgt gagaatttcc tcaaagcagg caccgcctgc ttcgttaacg
    10561 acagggtaga aaggtagaag ttatgattcg cacgatgctg cagggcaaac tccaccgcgt
    10621 gaaagtgact catgcggacc tgcactatga aggttcttgc gccattgacc aggattttct
    10681 tgacgcagcc ggtattctcg aaaacgaagc catcgatatc tggaatgtca ccaacggcaa
    10741 gcgtttctcc acttatgcca tcgcggcaga acgcggttcg agaattattt ctgttaacgg
    10801 tgcggcggcc cactgcgcca gcgtcggcga tattgtcatc atcgccagct tcgttaccat
    10861 gccagatgaa gaagctcgca cctggcgacc caatgtcgcc tattttgaag gcgacaatga
    10921 aatgaaacgt accgcgaaag cgattccggt acaggttgct tgattgttat ccctgcggct
    10981 ggttactcac cagccgcgac atcgtctcca gcgaatccgt tcttaagata taaagtcgtt
    11041 ttagtaacaa cggattatcc cccggtttta ctttgccttt catggttgtc accgccaggt
    11101 gaaatccggc atcgtttgct gccttcacgg ctttgtcatt aaatccgcca aacggatacg
    11161 aaagatacca gacatgcgga ttaaattgcg ccagagcgcg gcgtgaacgt gcaaaatcaa
    11221 acagaatatt gtgctcacta cggctcagta atatgggtcg gcgatagcca tccacccgat
    11281 gcaaaaaatg ggtatgtgac tgaaaatcaa aaacatcgcg aatttcgtta agctcagaaa
    11341 cgctcataaa ttgcagcgat tttgggttcc acttctgcgg gtgacgtttg atgcgcgagg
    11401 taacaacaaa cgccgtcgcc ttcatgccat attgtttcaa tacaggatac gcatagcggc
    11461 tcaccgactt gaggccatca tcaaaggtaa tcaccaccgc tcgcgcaggg agattgatct
    11521 tattcttcac gtaaccttcc agctgcgcca tgctcagtgt cgcgtatccc ctgtcgcgca
    11581 gccaggccat ctggttattg aaagcgcgta ccgaggtggt cgtcgaagta tggcgaaaac
    11641 gggtgttttc ttcatcgcgc agaatatggt gataggttag caccggcagg ccattatcgg
    11701 gttgggcatc cagcgcgctg atataggcga gtcgatcgcc aatacggatc tgataccagg
    11761 tttgatttaa cctgtctttc agtttatgca aaatcgggta gcgcaaattg tccgccagta
    11821 ccccaaatgg cgcactgccc gcactcggcg cgttatagac cggcgtatct ttccaggtaa
    11881 ttaagttctg attactcagc ggcttgttga ggtcacccaa accgtcttca actttttgtc
    11941 gcccctgaac tggctcgaga tgccctttat cgataaaccc tttaccaaag ccaaaattaa
    12001 atgcgtaata acttgcggca gtgggctcca cggcaatgat ttgcccggcc cgaatattcc
    12061 ccacggtcac catcttgtcg ccaatttgcg cccagatcgc agcattttcg atggtttgca
    12121 tataacgggc aggtaacgcg gcactgacac ttgcggtaaa cagcatcagc aggagaataa
    12181 cagcttgttt gtacatgaaa aaataaccga gggtgaaaca acgcttttat tgtagcaaaa
    12241 gcgctataag aatcgtgggt taatactcat acaaattatg tttgaggacg aaaggcggat
    12301 ttttttgttg ttggtgccaa agactactca cctccggccc acccagttcg acaatacgct
    12361 cgcgaaactc tgaactggtc atggtttcac ggcaagccat catctgttca attaacggta
    12421 acgtgacacc agaaatcact tcgcaacggg agtgtttgtg gcttaataat gaagccacgc
    12481 ggtacggtgg cgctccggct atatcggtta agaagatgac accctcacca gagtccgctt
    12541 catgcagagc atcgcacatc atgcggctga gcatattaga gcttaaccca cgccagaaat
    12601 tcacggcccg gcactgaaga agcgccccat gttttttctc cagcgcatcc agtatctctt
    12661 gcgcccggtc atcgtgacag gtaattaccc aacctaacat cgccttcctc cttagccgaa
    12721 aatcagtcta ccctgaccat tgttgataaa gggtgatagc cgtcaaaact cctctccccc
    12781 aaatccagag gagaggaaat agccttagct acgcaaacca cgtccacgtt ggatcagcga
    12841 ccaacagatc aaataaaacg ccacaataaa gaccaccagt acgccaaagg tagtgaccag
    12901 cggaacatca ttgataccga ggaagccgta gcggaaacca ctgatcatat aaacgattgg
    12961 gttcaggtgc gacagccctt gccagaacgg cggcaacaaa gtcagtgagt aaaagacccc
    13021 acccaaatac gtgagtggcg ttaacacaaa ggttggcacc aggctgatgt catcgaacgt
    13081 tttggcaaac acgccgttca gcaaacccgc aagggagaat agcaccgccg tgagcaccag
    13141 ggttaaggca acgaataccc acgaatgcac ctgaaacggc acaaaaaaca gtgaaattgc
    13201 cgtcaccaga atgccaacaa acagaccacg cgccacgcca ccgccgacat atccggcaat
    13261 aatgacgtga gtcggaaccg gcgctaccag cagctcttca atattacgct ggaacttggc
    13321 accaaaaaat gatgacgcga cgttagcgta ggcattggtg atcaccgaca tcatgatcag
    13381 ccccggtacg atgaactgca tatagctgaa gccatgcata tcgccaatac gcgaacctat
    13441 caggttaccg aagataataa agtaaagggt catggtgatg actggcggca ccagcgtctg
    13501 cacccagata cgcataaagc gatggatctc tttcgcccag atgcttttta gcgccaccca
    13561 gtaaagatgc atcatgcgcg atctccttgt ttttcattaa ccagtgaaac aaacagctct
    13621 tccagacggt tagctttgtt acgcatactt aatacctgaa tgccctgctc acttaactgc
    13681 gtaaatacgc tgttgatccc ctgctcacgc agcacttcaa cttccagcgt cgcggtatcg
    13741 accagtcgat actgatagcc atcgagcttc ggtaacggac ttttcggtgc gagatcgaga
    13801 ataaaggttt ccgatttcag cttagccagc agcgccttca tcgaggtatt ttccaccagc
    13861 tcaccgtgtt gaataatgcc gatattgcgg cacagcattt ctgcttcttc caggtagtgt
    13921 gtggtgagaa tgatggtggt gcctttgtcg tttaaatcct tcaaaaagcc ccacattgag
    13981 cggcgaagtt caatatccac gcctgcggtc ggttcgtcga gaatcagtag tttaggttca
    14041 tgcattaacg cacgggcaat cattaaacgg cgcttcatcc cgccagataa catacgcgca
    14101 cgttcgttgc gttttcccca tagatcgagt tgtttaagat atttttcgct gcggatgtac
    14161 gcctctttgc gctccacacc gtagtaccct gcctgattta ccacaatttg ctgcacggtt
    14221 tcaaacgggt tgaagttaaa ttcctgcggc accagtccca actgacgttt agcgttcacg
    14281 acatctttct cgagatcgta accaaatacg ctgacccgcc cggaggtttt atttaccaga
    14341 gagctgataa taccgatagt ggtcgatttc ccggccccgt tcggcccgag aagcgcataa
    14401 aaatcacccg cttcgacctg caaatctatt ccacgaagcg cctgaacgcc gcctggataa
    14461 gtttttttaa gctgttgaag ttccagtgca atggtcataa atttttactt accttacgtt
    14521 cttacacttt atctgtggtt taaatcgtcc cggagttgcc ctatattagc caaacgtaat
    14581 tatttggtta caggtcgtta acctccatga aagacataga tacactcatc agcaacaatg
    14641 cactatggtc aaaaatgctg gtggaagagg atcccgggtt ttttgagaaa ctggcacaag
    14701 cgcaaaaacc gcgctttcta tggattggat gttccgacag tcgcgttcct gcagaacgtt
    14761 taaccggtct tgagccgggc gaactctttg ttcaccgtaa tgttgctaac ctggtcattc
    14821 acaccgacct gaactgcctt tccgtggttc agtatgcagt ggatgtactc gaagttgaac
    14881 acattattat ctgtggccac tacggttgcg gcggcgtaca agccgccgtt gaaaacccgg
    14941 aactggggct tatcaacaac tggctgctgc atatccgcga tatctggttc aaacatagct
    15001 cattgctcgg cgaaatgccg caagagcgcc gtctggatac cttgtgtgaa ctgaacgtca
    15061 tggaacaggt gtataacctg ggccactcca ccattatgca atcagcgtgg aaacgcgggc
    15121 agaaagttac cattcacggc tgggcctacg gcattcacga cggcttgctg cgtgatctgg
    15181 atgttaccgc caccaaccgc gaaacccttg agcaacgtta ccgtcacggg atttccaacc
    15241 tcaagctgaa acacgccaac cacaaataaa aatgccatgc cggatgcaac acatccggca
    15301 acttcacact tactcgtcca gcagaatcac tttgccgata tacggcagat gacggtaacg
    15361 ctgtgcgtaa tcaatgccgt aacccaccac aaactcatcc gggatcgaga aaccgataaa
    15421 ttctaccggg acgttcactt cacgacggga cggtttatcc agcagcgtac aaatcgccag
    15481 cgacttcggt tcgcgcaggc ttaagatctc acgcactttc gacagtgtat tccccgagtc
    15541 gatgatatct tcaacaatca gcacgtcctt gccacggata tcttcatcca gatctttgag
    15601 gattttcaca tcacgggtgg tggacatgcc gctaccgtag ctggaggcgg tcataaagtc
    15661 gacttcatga gatacctgaa cttcacggca caggtccgcc ataaacataa atgagccacg
    15721 cagcagaccc accagcacca tatcgctgcc gctgtctttg taacgctcag taatctgacg
    15781 acccagttcg gcgatacgcg ctttaatctc cgcttcgggg atcattactt ctacagtatg
    15841 tttcatatct ctaaccatat gatttaaaaa caaatcactt cgcacgggca ggttttggca
    15901 ccgcaacaaa gcgcaagaca cgcagtatac cagtaaaact attcattcgc ggaatctgtt
    15961 aataaagctc gaattgtgat gacgatcaca catgttaaaa cctataatta attgctacta
    16021 aaatattaat gaattgaaat ggtgtctctt tatggcaatt aacaatacag gctcgcgacg
    16081 attactcgtc acgctaacag ccctttttgc agcgctttgc gggctgtatc tactcattgg
    16141 cggaggctgg ctggtcgcga ttggcggctc ctggtactac cctatcgctg gccttgtgat
    16201 gctcggcgtc gcctggatgc tgtggcgcag taaacgtgcc gcgctttggc tgtacgcagc
    16261 cctgctgctc ggcaccatga tttggggcgt ctgggaagtt ggtttcgact tctgggcgct
    16321 gactccgcgc agcgacattc tggtcttctt cggcatctgg ctgatcctgc cgtttgtctg
    16381 gcgtcgcctg gtcattcctg ccagcggcgc agttgccgca ctggtggtgg cactgctgat
    16441 tagcggtggt atcctgacct gggccggatt taacgatccg caggaaatca acggcacctt
    16501 aagcgccaat gccacacctg cggaagctat ctcacccgtt gccgatcagg actggcctgc
    16561 ctatggtcgc aatcaggaag gtcaacgctt ttcacctctg aaacaaatta acgccgataa
    16621 cgtccataat ctgaaagaag cctgggtgtt ccgtactggc gatgtgaagc agccgaacga
    16681 tccgggcgaa atcaccaatg aagtgacgcc gattaaagtg ggcgacaccc tttacctgtg
    16741 taccgctcac cagcgcctgt ttgcgctcga tgccgccagc ggcaaagaga aatggcatta
    16801 cgatcctgag ctgaaaacta acgagtcttt ccagcacgta acctgccgtg gcgtctctta
    16861 tcatgaagcc aaagcagaaa cggcttcgcc ggaagtgatg gcggattgcc cgcgtcgtat
    16921 cattcttccg gtcaatgatg gtcgactgat tgcgattaac gctgaaaacg gcaaactgtg
    16981 cgaaaccttc gccaataaag gcgtgctcaa tctgcaaagc aatatgccag acaccaaacc
    17041 gggtctgtat gaaccgactt cgccaccgat tatcaccgat aaaaccatcg tgatggccgg
    17101 ttcagttacc gataacttct caacccgcga aacgtctggc gtgatccgtg gttttgatgt
    17161 caacaccggg gagctgctgt gggcttttga tcccggcgcg aaagatccga acgcaatccc
    17221 gtctgacgaa cacaccttta cctttaactc gccaaactcc tgggcaccag cggcctatga
    17281 cgcgaagctg gatctggtct atctgccgat gggcgtgacc acgccggata tctggggcgg
    17341 taaccgcaca ccggaacagg aacgttatgc cagctcgatt ctggcgctga atgccactac
    17401 cgggaaactg gcgtggagct accagaccgt tcaccacgac ctgtgggaca tggatcttcc
    17461 ggcacagccg acgctggcgg acatcaccgt taatggtcag aaagtgccag ttatttacgc
    17521 tccggcgaaa accggcaaca tttttgtgct cgatcgtcgc aatggcgaac tggtggttcc
    17581 ggccccggaa aaaccggtgc cgcaaggtgc tgcgaaaggc gattacgtta ctccgaccca
    17641 gccgttctct gaactgagct tccgtccgaa gaaagatttg agcggtgcgg atatgtgggg
    17701 agccaccatg tttgaccaac tggtgtgccg cgtgatgttc caccagatgc gctatgaagg
    17761 cattttcacg ccgccatctg aacagggcac gctggtcttc ccgggtaacc tgggaatgtt
    17821 tgaatggggc gggatttccg ttgatccaaa tcgtgaagtg gcaattgcca acccaatggc
    17881 actgccgttt gtttcgaaac tgatcccacg cggtccgggt aacccgatgg agcagccgaa
    17941 agatgccaaa ggcacgggta cggaatccgg cattcagcca cagtacggtg taccgtatgg
    18001 tgtcacgctc aacccgttcc tctcaccgtt tggtctgccg tgtaaacagc cagcatgggg
    18061 ttatatctcg gcgctggatc tgaaaaccaa tgaagtggtg tggaagaaac gtattggtac
    18121 gccgcaagac agtatgccgt tcccgatgcc tgtaccggtg ccgttcaata tgggtatgcc
    18181 gatgctgggc gggccaatct ccacggcggg taacgtgctg tttatcgctg ctacggcaga
    18241 taactacctg cgcgcttaca acatgagcaa cggtgaaaaa ctgtggcagg gccgtttacc
    18301 agcgggtggt caggcgacgc caatgaccta tgaagtgaat ggcaagcagt atgtggtgat
    18361 ctccgcaggc ggtcacggtt catttggtac gaagatgggc gactatattg tggcttatgc
    18421 gctgccggat gatgtgaaat aaccataatg cccggagaga tccgggcata tttccgaata
    18481 cggtcttttt ataccgtaaa ccctaacatc atccccgtat cttcatgctc cagcagatgg
    18541 cagtgcgcca tataagcatg ttctttcggt gcgttgtgat taaacttcac cagcacttca
    18601 ctgacattgc cttctacctt aacggtatct ttccagcccg cgcgatgagc cgctggcggt
    18661 ttgccatttt ctgacaagat acggaactgg gtgccgtgaa tatggaacgg atgcagcatc
    18721 atgtcgccca cgccagagat aacccaacgt tcgtactgcc ctttcgccgc cgcaaacatc
    18781 ggcttattca tatcaaacgc ctgaccgttg attttattgg catggtggaa atcgaacttc
    18841 ccgccatggt tcatatgatt catattgccg tgccccatat ggcccatcat ctggctatga
    18901 tccatcccgg ccatcgcctg atcgccatat ttctccatca gcatctgcat ccccatcata
    18961 tcgagcatcg ggtccataga aagttgcagc ttgcgtaccg tcagcccttc cagcgaaggt
    19021 aacgcaggca ggctacttaa tgtgtctggc aaagcaccgg aggcactaat agcaatcggc
    19081 tgaatccgca ttaccggatg cggcttgtca aacggcgcaa tcgccatccc catctggctg
    19141 accggtagcg tcaccaggtc aaagggtttg ttgtcgttaa cctccaccag cacttcaaaa
    19201 cgctcgccca tcagcaccgg cagctcgctc accttcaccg gttcaggtag cagaccaccg
    19261 tcgctggcaa tcacatacaa cgggcgattg tcgctggtgg cgaaattgag cgagcgggca
    19321 ttacagccat tgagcaaacg caggcgcagc caaccacgtg gggcagcgtg ttgtgggtag
    19381 attgcaccgt tggtcagcag cgtatcgcca aaccagccca cggcggcggt catcacatcc
    19441 agttgataat caatctgccc atcagcgtta aattttttat cctgaacaat caccggaaca
    19501 tcatcgatac cccactgttt tggcagcatt aatttcagga tctcgtcatc ttcaattacc
    19561 accagcccag ccaaccccat cgccacctgt cgcccggtct tgccatgttg atgtggatgg
    19621 aaccagcaag tagcggcagg ttgatcaacg ttcaacgtca ccgagcgctt gccacctggc
    19681 ggaataattc cctgcgggcc gccgtcgact tcacccggta cttccagccc gtgccagtgc
    19741 aacgtcgtct cttccgtcag ttggttatag atatcaaccg ttaccgcttt gccgcgctgt
    19801 aatttcaccg ccggccccag cagattgccg ttatagcccc aggtagttgc agttttctcg
    19861 ccaaaggtag actgacctgc gccaatagtt aactgaatgc gattacgggc atcggtcgtg
    19921 agcaaatcag ggatcggtaa cgttgggcgt tccgccgcaa agactgcgcg gctccacagc
    19981 ggcaaggctg aagccacacc cagcgcgacg gaatatttta agaaatcacg acgttgcata
    20041 gttatttcct tattcttaag caggcgaaac aaaatcaaac gttgagcata gtccttcccc
    20101 ttacgggaag gtcaagccgc agacacaatt taatcgacaa taatggtcgt cgcctcgctc
    20161 gcagtgtgct aacgtttatc ttctttaagc ccctggtaga agcaatgaag acgtttttca
    20221 gaacagtgtt attcggcagc ctgatggccg tttgcgcaaa cagttacgcg ctcagcgagt
    20281 ctgaagccga agatatggcc gatttaacgg cagtttttgt ctttctgaag aacgattgtg
    20341 gttaccagaa cttacctaac gggcaaattc gtcgcgcact ggtctttttc gcccagcaaa
    20401 accagtggga tctcagtaat tacgacacct tcgacatgaa agccctcggt gaagacagct
    20461 accgcgatct cagcggcatt ggcattcccg tcgctaaaaa gtgcaaagcc ctggcccgcg
    20521 attccttaag cctgcttgcc tacgtcaaat aatccctgat acctttttgt agaaatattg
    20581 accgtgcatc cgcggtcaat gttagctatt atgttgcgcc ctttttttac gggtgttaac
    20641 aaaggaggta tcaacccatg gccgaaaaaa aacagtggca tgaaacgcta cacgaccagt
    20701 ttgggcagta ctttgcggta gataacgttc tgtatcatga aaagaccgat caccaagatc
    20761 tgatcatttt tgagaacgct gcatttggtc gcgtaatggc gctggatggc gtagtacaaa
    20821 ccaccgagcg cgacgagttt atctatcatg agatgatgac ccatgttccg ctactggccc
    20881 acggtcacgc gaaacatgtg ctgattatcg gcggcggcga cggtgccatg ctgcgtgaag
    20941 taacccgaca taaaaacgtt gagtcaatca cgatggtgga aatcgatgcg ggtgtcgtat
    21001 cgttctgccg tcagtatcta cccaaccata acgccggtag ctacgacgat ccgcgcttta
    21061 agttggtgat cgacgatggc gtcaatttcg ttaatcaaac cagccagacc tttgatgtca
    21121 ttatctccga ctgcaccgat cctatcggtc ccggcgaaag ccttttcact tcggcatttt
    21181 atgaaggctg caaacgttgc ctgaatcctg gcggtatctt cgtcgcacaa aacggcgtct
    21241 gctttttaca gcaggaagaa gccatcgaca gccatcgcaa actcagccat tacttcagcg
    21301 acgttggctt ttatcaggcg gcgattccga cctattacgg cggtatcatg acttttgcat
    21361 gggcgacaga taacgacgcc ttacgccatc tctcaaccga aattattcag gcgcgttttc
    21421 tcgcttctgg cctgaaatgc cgttattaca atccggcaat ccatacggca gcttttgcct
    21481 tacctcagta tctgcaagac gcactggctt cacagccgtc ctaaggagaa gataagaaat
    21541 tgaaaaaact gaaactgcat ggctttaata atctgaccaa aagtctgagt ttttgtattt
    21601 acgatatctg ctacgccaaa actgccgaag agcgcgacgg ttatattgct tatatcgatg
    21661 aactctataa tgccaaccgt ctgaccgaaa tcctgtcaga aacctgttcc attatcgggg
    21721 ccaatattct taacatcgcc cgccaggatt acgaaccaca gggtgccagc gtcactattc
    21781 tggtgagtga agaaccggtc gacccgaaac ttatcgacaa aacagaacac cccggcccac
    21841 tgccagaaac ggtcgttgcc catctcgata aaagtcatat ttgcgtacat acctacccgg
    21901 aaagtcatcc tgaaggcggt ttatgtacct tccgcgccga tattgaagtc tctacctgcg
    21961 gcgtgatttc tccgctgaag gcgctgaatt acctgatcca ccagcttgag tccgatatcg
    22021 taaccattga ttatcgcgtg cgcggtttta ctcgcgacat taacggtatg aaacacttta
    22081 tcgaccatga gattaattcg attcagaact ttatgtctga cgatatgaag gcgctgtatg
    22141 acatggtgga tgtaaatgtc tatcaggaaa atatcttcca taccaagatg ttgcttaaag
    22201 agttcgacct taagcactac atgttccaca ccaaaccgga agatttaacc gacagcgagc
    22261 gccaggaaat taccgctgcg ctgtggaaag aaatgcgcga gatttattac gggcgcaata
    22321 tgccagctgt ttaacgtctc gggcggagct cccaggctcc gccagattta tttacttctg
    22381 ctgcacgaaa ttacggtaag ccgccacgac ctgcagaaaa tcctcaacgc cgcatagcga
    22441 caggctttct tcgtcgtagt agttcatccc ctcttccatt tcatcgccag cgaattccag
    22501 ttgattggcg cgaacaatca cctcttcacc gtccatccac agagtgtact catgccctgc
    22561 ccgctgccag gaacgttcgc tacctttcag tgcgtgcgcc gcttgttcca cttcatcaag
    22621 caaggccagg ttttctttca cctcttcatt aaaccagtgc ccgaccactt catgccccat
    22681 ggacatacgc acctttacaa ctccggtaat atcgcgcaga aattcgtaat ccatagtgtg
    22741 ttcctctgct cccggcaatg cgccgaaatc gcattgcgcc attgatgtaa ttatcgcagc
    22801 cttaccggag aaaaacagcg gaacggcgat ggtttagaaa taaaaaaatg cccgggaatc
    22861 tcattcaccg ggcattgtgt cgtttatgcg cagcgcgtgc gcttacttat tacaccgcag
    22921 tctggaaaat caccccatcg gctttttcgg tgtactgaga aagctggttg aagttcagat
    22981 aacgataagt atcaacggca gttttatcta cttgcgccac gtaggtctgg tactcttccg
    23041 gcgtcggcag tttgccaatc agcgccgcaa caaccgccag ttccgcagaa gccaggaaga
    23101 cattcgcgcc agtacccaga cggttcggga agttacgggt agaggtggaa accaccgtcg
    23161 caccgtctgc tacacgcgcc tggttaccca tacacaggga acagccaggg atctcgatac
    23221 gcgcaccact cttaccgaag acgctgtagt agccttcttc agtcaactgc gcggcgtcca
    23281 tacgggttgg cggtgccacc cacaggcggg tcggcagttg gcctttgtgc gcatccagca
    23341 gtttacccgc agcacggaag tgaccgatgt tggtcatgca ggaaccgata aatacttcgt
    23401 cgatcttctc accctgtacc gcagacagcg gacgcgcgtc atccgggtcg ttcggtgcac
    23461 agaggattgg ctctttaata tccgccagat cgatgtcgat cactgccgcg tattcagcat
    23521 ctgcatcggc ttccagcagc tcaggattcg ccagccattt ttccatgccc tgaatacgac
    23581 gttccagggt gcgacgatcg ccgtaacctt ccgcgatcat ccacttcagc aggacgatgt
    23641 tagagttcag gtattcgatg atcggttctt tgttcagctt gatggtacaa ccagcggcag
    23701 aacgctcggc ggacgcatcg gtcagttcaa atgcctgctc aactttcaga tccggcagac
    23761 cttcaatttc caggatgcgg ccagagaaga tgtttttctt gcctttcttc tcaacggtca
    23821 gcagaccttg tttgatcgca tacagcggaa tggcatgtac cagatcgcgc agggtgatgc
    23881 ccggctgcat tttgcctttg aagcgcacca gaacggattc cggcatatcc agcggcatta
    23941 cgcctgttgc ggctgcaaat gccaccagac cagaacccgc cgggaaagag ataccgatcg
    24001 ggaaacgggt atgggaatca ccaccggtac cgacggtatc cggcagcagc atacggttca
    24061 gccaggagtg aatgacgccg tcacccggac gcagcgacac accgccacgg ttcataatga
    24121 agtccggcag cgtgtggtgc gtgttcacgt caactggctt cggatacgcc gcggtgtggc
    24181 agaaagactg catcaccagg tcagccgaga agcccaggca cgccaggtct ttcagttcat
    24241 cacgggtcat cgggccggtg gtgtcctggg aacctacaga agtcattttc ggttcacagt
    24301 acgcgcccgg acgaatgcct ttcacgccac aggcacggcc taccattttt tgcgccagcg
    24361 agaagccgcg atcgctctca gcgacatctt tcgcctgacg gaacacatca ctgtgcggca
    24421 gaccaagtgc ttcacgcgct ttggtggtca ggccacgccc gataatcagc ggaatacggc
    24481 caccagcacg cacttcatca atcagcacgt cggttttcag ttcgaaggtc gccagcagtt
    24541 cgccggtttc gtggttacgc acttcacctt tgtacgggta aacgtcaatc acgtcgccca
    24601 tgttcaggtt agagacgtcg acttcgattg gcagtgcacc cgcgtcttcc atcgtgttaa
    24661 agaagatggg tgcaatttta ccgccgaggc acaaaccacc gccgcgtttg ttcggcacat
    24721 gtggaatatc atcgcccata aaccacagaa cggagttagt ggcggattta cgcgaagaac
    24781 ccgtacccac aacgtcaccg acgtacgcca gcgggaaacc tttctgttgc agagcttcga
    24841 tttgcttgat cggaccaaca acaccaggct ggtctggctc aataccttca cgggcgtttt
    24901 tcagcatcgc cagcgcgtgc agtgggatat ccgggcgtga ccacgcatcc ggtgccggag
    24961 aaaggtcatc ggtgttagtt tcgccagtga ctttgaagac ggtaacggtc agtttttcag
    25021 ccagcgccgg gcgattcagg aaccattcgg catccgccca ggactgcata acctgcttcg
    25081 catattcgtt gcctgctttc gctttctctt ctacgtcata gaagttatcg aacatcagca
    25141 gcgtgtgaga aagtgctttg gcagcaatag gtgccagttt ggcatcatcc agcgcgtcga
    25201 tcagcggatg aatgttgtaa ccaccctgca tggtgcccag cagttcgatg gctttttccg
    25261 gagtcagcag aggggatttg gcttcgcctt tcgcgatagc agccaggaag cctgctttga
    25321 cataggcggc ttcatcgacg cctgggggaa cacggttggt taacagatct aacaggaatt
    25381 cttcttcgcc cgcgggcggg tttttcagca gctctacaag tgcggccatt tggtttgcat
    25441 ccaggggttt gggcgcaatc ccctcagcgg cacgctcagc tacgtgctta cggtattctt
    25501 ctagcacgac ggttctcctc gctctcattg tcatagtgcg gcaggcgatt ctcttcacgc
    25561 tcctgtgaga cagcagtttg tagggtaaat gcccagtacc gcaacgggca gaatagcagg
    25621 attttgacga tgtgttaatc tgtttacaaa aaagcaacat aaaaaagtgg ctgaatcgtt
    25681 aaggatggtc tagagattgt ttccctccgt aaaaacttcg ttaattcgca tggtgataat
    25741 cacctttctc aacgaatccc aagattagtc aaaatttaaa ctaccgcctc tttatactcg
    25801 gattcacagc acctgcgggt ggcagttcgc cgaccattgc gatttccttg agatccgaat
    25861 tatgaaaatg actttgccgt ttaaacccca tgtgctggcg ctaatttgca gtgccgggct
    25921 ttgtgccgcc tctaccgggc tatatataaa aagccgcaca gtggaagcgc ctgtggaacc
    25981 gcaatcgaca caacagactg cgcctgacat caccgcagtt acgcttcctg caacggtttc
    26041 cgcaccgccc gtaacgcccg ccgtcgtcaa atccgcattc agcactgcac aaatcgatca
    26101 atgggttgcg cctgtcgcgc tgtaccccga ttctctgctt tcacaagtgt taatggcatc
    26161 aacctatccg gcaaacgttg ctcaagcagt gcaatggtcg cacgataatc cacttaaaca
    26221 aggcgatgct gctattcagg cggtatctga ccagccgtgg gacgccagcg ttaaatcact
    26281 ggtggccttt ccacaattga tggcattgat gggcgaaaac ccgcaatggg tgcaaaacct
    26341 gggcgatgct tttctggcgc agccgcagga cgtgatggac tcggtacagc gattgcggca
    26401 actggcgcaa caaaccggtt cgctgaagtc atcaaccgaa caaaaagtta ttaccacaac
    26461 gaagaaagct gtaccggtaa aacagacagt cacggcaccc gtcataccat ccaataccgt
    26521 tttaactgcc agccccgtca ttacagagcc tgcaacaacc gtcatttcca ttgagcccgc
    26581 caatcctgat gtggtctata ttcccaacta caacccaacc gtggtttacg ggaactgggc
    26641 caatactgcg tatccgccgg tttatctgcc accaccagcc ggagaaccgt ttattgacag
    26701 ctttgtgcgc ggattcggct atagcatggg tgttgctacc acgtacgcac tattcagcag
    26761 catcgactgg gatgacgacg atcatgacca tcatcatcat gacgatgatg attatcatca
    26821 ccacgatggc ggtcatcgtg acggtaatgg ctggcaacac aacggcgaca acatcaatat
    26881 cgacgtcaac aatttcaacc gtatcaccgg tgagcatctt actgataaga atatggcatg
    26941 gcggcacaat ccaaactacc gtaatggtgt gccctatcat gatcaggata tggcaaagcg
    27001 gtttcatcaa accgatgtca acggcggaat gagcgccacg cagctacctg ctccaacacg
    27061 cgacagccag cgtcaggcgg cagcaagtca gtttcagcaa cgaacacacg ccgcccccgt
    27121 cattacacga gatacccaac gtcaggcagc ggcacagcgg tttaatgaag ctgaaaacta
    27181 tgggagctat gacgacttcc gcgacttcag ccgtcgccaa ccactgaccc agcaacaaaa
    27241 ggacgccgct cgtcagcgtt atcagtcggc ctcgcctgag cagcgccagg cagtccgcga
    27301 gaaaatgcag actaacccac agatccagca gcgaagagac gcagcgcgtg agcgtattca
    27361 gtcagcctcg cctgagcagc gccaggtgtt taaggaaaaa gtacagcagc gcccactgaa
    27421 ccaacagcaa cgtgataacg cccgccagcg tgttcaatca gcatcacctg aacaacgtca
    27481 ggtttttcgg gaaaaagttc aggagagccg cccacaacgt ctaaacgaca gtaaccatac
    27541 tgtcaggctg aataacgagc aacggtcagc agtacgcgaa cgtctctctg agcgcggagc
    27601 aaggcgacag gaaaggtaaa ttgcagacgt aaaaaaagcg gcgtggttag ccgctttttt
    27661 aattgccgga tgttccggca aacgaacaat tacttcttct tcgctttcgg gttcggcagg
    27721 tcggtaatgc taccttcgaa cacttctgcc gccaggccca cagactcgtg cagagtcggg
    27781 tgcgcgtgga tggtcagtgc gatgtcttca gcatcacaac ccatttcgat tgccaggccg
    27841 atttcaccca gcagctcgcc gccgttggta ccgacaatcg caccaccgat cacacggtga
    27901 gattctttgt cgaaaatcag cttggtcata ccgtctgcgc agtcggaagc gatagcacga
    27961 ccagaagcag cccacgggaa ggtggcggtt tcatagctga tgcctttctc tttcgcttct
    28021 ttctcagtca gacctaccca tgcaacttct ggttcggtat aggcgatgga cgggataact
    28081 ttcggatcga agtagtgttt cttaccggcg ataacttcag cggcaacgtg accttcgtga
    28141 acacctttgt gtgccagcat cggctgaccg acgatatcgc cgatagcaaa gatgtgcggt
    28201 acgttggtac gcagctgttt gtcaacgcgg atgaaaccac ggtcgtccac ttccacgcca
    28261 gctttgcctg cgtcgaggtt tttaccgttc ggcacacgac caatcgctac cagcacggcg
    28321 tcgtaacgct gcggttcagc gggtgctttt ttgccttcca tcgtcacata aataccgtct
    28381 tctttcgctt caacggcggt aactttggtt tccagcatca ggttgaattt cttgctgata
    28441 cgcttggtga agactttaac gatgtctttg tcagctgccg ggataacctg gtcgaacatt
    28501 tcaaccacgt caatctgtga acccagcgcg tggtatacgg tgcccatttc cagaccgatg
    28561 ataccgccac ccattaccag caggcgttct ggtacttctt tcagttccag cgcgtcagtg
    28621 gagtcccaga tacgcggatc ttcatgcgga ataaacggca gttggatcgg gcgagaaccc
    28681 gctgcaatga tcgcattgtc gaagttgatc acggttttgc cgttctcacc ttcaacttcc
    28741 agggtgttag ccccggtgaa tttacccaga ccgttgacca ctttgacttt gcggcctttc
    28801 gccataccag ccagaccacc ggtcagctga ttgatcactt tctctttcca ggtacgaatc
    28861 ttgtcaatat cggttttcgg ttcgccgaag acgataccgt gttcagccag cgctttggct
    28921 tcttcgataa cttttgctac gtgcagcagt gctttagaag ggatacagcc gacgttcagg
    28981 caaacaccgc caagggtgtt gtaacgttct acgattacgg tttccagacc taaatcagcg
    29041 caacggaagg cagcggagta acctgcgggg cctgccccaa gtaccacgac ctgagttttg
    29101 atttcagtac tcatcatgac ctctatatat ttatctccgg cggtcatacc cgtcgtcttt
    29161 caggccgcag cggcgttagc ttcacgccct caccccagtc acttacttat gtaagctcct
    29221 ggggattctg gcgcttgcta ccttgctgca cccagaaatc catagggtat gtgaagtaat
    29281 accctaacca ccaccgggtc gttctatccg tcggtatttt acaaaattgt taacaatttt
    29341 taaacaacaa acggcaaccg atttgtctat tcgctaataa cctcaataca ttcatgagat
    29401 taccagaaaa aagccggccg ttgggccggc tcttttactt acatcaccag acggcgaatg
    29461 tcagacagcg tgttgttaat gatggtaatg aaacgggcac catcagcacc gtcgatcacg
    29521 cggtggtcga aggagagaga aatcggcagc atcagacgcg gcacgaactc tttaccattc
    29581 cacaccggct ccatcgcgga cttggaaacg ccgaggatag ccacttccgg cgcgttcaca
    29641 atcggcgcga agtgggtagt acccaggccg ccgatgctgg agatggtgaa gcaaccgccc
    29701 tgcatttcgc ccgcagtcag cttaccgtca cgcgctttct tagaaatagt catcagctcg
    29761 cgagacagct cgatgatgcc tttcttgttg acgtctttga ataccggaac aaccagaccg
    29821 ttcggggtat ccaccgccac accgatgttg atgtatttct tcagggtcag acgctgaccg
    29881 tcttccgaca acgaactgtt gaagcgaggc atctgctcaa gagctgcagc aacggctttc
    29941 atgatgaaga caaccggggt gatcttcaca tccagcttac gtttcgccgc ttcttcgttc
    30001 tgctgtttac ggaacgcttc cagctcggtg atatcggttt tgtcgaagtg agtaacatgc
    30061 gggatcatca cccagttacg gctcaggttc gcaccagaaa ttttctggat acggcccagt
    30121 tccacttctt cgatttcacc aaacttgctg aagtccacct tcggccacgg cagcatgcct
    30181 gggataccgc cgccagtcgc cgccggagct gcttctgcac gtttgatagc ttctttcacg
    30241 taagcctgaa cgtcttcgcg caggatacga cctttacggc cagtgccctt cactttcgcc
    30301 aggttaacgc caaactcgcg tgccagacgg cggatcagcg gagtcgcgtg aacgtaagcg
    30361 tcgttttctg caaattcaga tttgccttcc gctttcgcag ccggtgctgc tgccggagct
    30421 tcagcttttg ctgccggagc cggcgctgcc gcttcctgtt tcgcaggagc tgccgcaggc
    30481 gctgcgcctt caacttcgaa gatcataatc agcgagccag ttttcacttt atcgccaacg
    30541 ttgactttca gttccttcac gacgcctgca aacggagccg ggacttccat agaagcttta
    30601 tcgccttcta cggtgatcag tgactgttca gcggcaattt tgtcgcccac tttcaccatc
    30661 acttcggtca cttcaacttc gtcaccgccg atatccggaa cgttaacttc tttcacgcca
    30721 gccgctggtg caggggccgc tgccggagcc gcttcctgtt tagccgccgg agctgccgcg
    30781 cctgcttcac ccgcgacttc gaagaccata atcagcgagc cggtagacac tttgtcaccc
    30841 acgttcactt tgatctcttt cacggtgcca gcaaacggag ccggaacttc catagaagct
    30901 ttatcgcctt ctacggtgat cagcgactgt tcagcttcaa ctttatcgcc aactttcacc
    30961 aggatttcgg tcacttcaac ttcgtcgctg ccgatatccg gaacgttaac gtcttttgcc
    31021 gctgcagccg ctggtgctgc tgccggagct gtttctttct tctcttctgc ctgagcaggt
    31081 gcagcgtctg ctgcaccgtc ggcggaatcg aaaatcataa tcagtgcgcc ggtctgggtt
    31141 ttatcgccaa cagagacttt gatctcttta acgatacccg cctgcggaga cggaacttcc
    31201 atagaggctt tgtcgccttc tacggtgatc agcgactgtt cggcttcaac tttgtcgccc
    31261 actttgacca ggatctcggt gatttcaact tcatcagccc cgatgtccgg tactttgatt
    31321 tcgatagcca ttattctttt acctcttacg ccagacgcgg gttaacttta tctgcatcga
    31381 tgttgaattt ggcgattgcg tcagcaacca ctttcttatc gatttcgcca cgtttagcca
    31441 gttcgcccag cgccgctaca accacgtagg aagcatcaac ttcgaagtgg tgacgcaggt
    31501 tctcacggct gtcggaacga ccgaagccat cagtacccag tacgcggtag tcgtcagccg
    31561 gtacgtaagt acggacctgc tcagcgaaca gtttcatata gtcggtagat gccactgccg
    31621 gagcgtcgtt catcacctga gcgatatacg gaacgcgcgg agtttccagc gggtgcagca
    31681 tgttccagcg ttcacaatcc tgaccatctc gcgccagttc ggtgaaggag gtcacgctat
    31741 aaacgtcaga acctacgccg taatctttcg ccaggatctc tgccgcttca cggacgtgac
    31801 gcaggatgga accggagccc agcagctgaa ctttaccttt gctaccttca atggtttcga
    31861 gtttgtagat acctttacgg ataccttcct cagcaccttc cggcattgcc ggcatgtggt
    31921 agttttcgtt cagcgtagtg atgtagtagt aaacgttctc ttgtttttca ccgtacatac
    31981 gctccagacc gtcatgcata atgacagcaa cttcgtaagc gtaagccggg tcgtaagaga
    32041 tacagttcgg gatagtcagc gactgaatgt ggctgtgacc atcttcgtgc tgcagacctt
    32101 caccgttcag ggtggtacga ccggaagtac cgccgatcag gaagccacgc gcttgctggt
    32161 cgccagccgc ccagcacaga tcgccgatac gctggaagcc gaacatcgag taatagatgt
    32221 agaacggaat catcggcaga ttgttggtgc tgtaagaggt cgccgctgcc agccaggaac
    32281 aacctgcgcc caactcgttg atcccttcct gcagaatctg acctttctcg tcttctttat
    32341 agtaagcaac ctgctcgcgg tcctgcgggg tgtactgctg accgttcggg ctgtaaatac
    32401 caatctgacg gaacagacct tccataccga aagtacgcgc ttcgtcggcg atgatcggta
    32461 ccagacgatc tttgatcgac ttgttcttca gcattacgtt cagggcacga acgaaagcga
    32521 tagtggtaga gatctctttg ctctgctctt ccagcagcgc gccgaagtct tgcaggctcg
    32581 gcagctcaag cttctcggtg aagttcggct gacggcttgg cagataaccg tgcagtttct
    32641 gacgctgtgc gtgcagatag gtatgctctt cagaaccttc cgggaaggtg atgtacggca
    32701 gtttttcgat atctgcatca gacaccggca cattgaaacg gtcgcggatg tgacgcacgc
    32761 cgtccatgtt cattttctta acctggtgcg cgatgttttt accttcagcc gcgtcgccca
    32821 tgccgtaacc tttaatggta tgagcaagga ttactgtcgc tttgcctttg gtttcctgcg
    32881 ctttcttgaa tgcagcgtag attttcttcg gatcgtgacc accacggttc agtgcccaga
    32941 tctgctcgtc agtccagtct gcaaccagtg ctgcggtttc aggatattta ccgaagaagt
    33001 gttcacgaac gtacgcacca tctttcgatt tgaaggtctg gtagtcgccg tcaacggttt
    33061 cgttcatcag ctggatcagt ttaccgctgg tatctttacg cagcagttca tcccaacggc
    33121 taccccacat cactttgatc acgttccagc cagcaccttc gaagatgcct tccagttcgt
    33181 tgatgatctt gccgttaccg gtgaccgggc cgtcaagacg ctgcaggtta cagttgataa
    33241 cgaagaccag gttatccagt ttttcacggg tagcgatggt gatcgcacct ttggattccg
    33301 gttcgtccat ttcaccgtcg ccgaggaacg cgtaaacggt ctgtttagag gtatctttca
    33361 ggccacggtg ttccagatat ttcaggaatt tagcctggta aatagcacca atcggcccca
    33421 gacccataga tacggtcggg aactgccaga attccggcat cagtttcggg tgcggatagg
    33481 aagagaggcc attgccgtga acttcctgac ggaagttatc cagctgctcc tgagtcagac
    33541 gaccttccag gaaagcacga gcgtacacgc ccggggagat gtggccctgg aagtaaacca
    33601 ggtcgccgcc atcctgctcg ttgcgtgcac ggaagaagtg gttaaagcac acatcataaa
    33661 tggttgcgga agactggaag gacgccatat ggccgcccag ttcgaggtct tttttcgacg
    33721 cacgcagcac cgtcatgatg gcgttccagc ggatagctga acgaatacgg cgttccagtt
    33781 ccagattacc cggatactcc ggttgttctt caacggggat ggtgttgatg tagttgctga
    33841 tacctgtgcc tgcggctacg tttacaccgc ctttgcgggc ttcagcaagc agttggtcga
    33901 tcagatactg agcacgctca acaccttctt cacggatgac cgattcgatc gcctggagcc
    33961 agtcgcgagt ttcgatcgga tccacgtcat ttgggaaacg ttctgacatg ggttattcct
    34021 tatctatcta ataacgttga gttttctgga acctgtccca ttgaactctc gccggaaagc
    34081 tcaataagac aggttctacg tttagttgcc gcgcttttat atgcgcttga tttacaacat
    34141 cttctggata atttttacca gaaaaatcac taattctttc gttgctccag acgacgcaga
    34201 gaacgctcac ggcggctctc ttcacgactt ctgtcgagca aaatttcttc gataaaggcc
    34261 agatggcgat gcgatgcttc gcgcgcttct tccggcttac cggccataat cgcttcaaat
    34321 atgcgggtgc ggtgactact caccagcggc agcatctcgc gacgcgaata gagcaattcg
    34381 aagttctggc ggacattctg ggccaacatc ggctccatac accttagcag atgaagcaga
    34441 accacattgt gggccgcttc ggtgacggca atctgatact ggagtacggc gtttgattcc
    34501 gcgtccagat cgccagactg ctgcgccagc tctatggcgt ggtggagttc acggatgcgt
    34561 tccttgtctt catcggtact acgcagcgcg gcgtaataag cggcgatacc ttccagggcg
    34621 tgtcgtgttt cgagcaagtc atactgtgac tcaggatggt cggagagcag ctccaccagc
    34681 ggatcgctga agctttgcca taggctgctc tggacaaaag tgccgccacc ctggcgacga
    34741 agcaacaagc ccttcgcttc gagacgttga atcgcctcac gcaaggaggg acgggagacg
    34801 tcaaactgtt ttgccagttc gcgttccggt gggagttttt cgcccgggcg gagagtgcct
    34861 tcgaggatca aaaactccag ttgctgctca atcacatcgg agagttttgg ttggcggatt
    34921 ttgctgtagg ccatgagttc ctgtcttaag ccacttgccg aagtcaattg gtcttaccaa
    34981 tttcatatct gtgacgctaa agtaacaaag tattcacctt atgtccatac aggttttgat
    35041 tgaaatcatg aaactgtgca cattttaaca acttgacata tataacgttt caaagttgta
    35101 actatgcaca aatgtagact ttacgtagga aaggagtttt ttaacgatta ttaaactata
    35161 aaaatccgaa ttgaaccgat tcacttacca attttgtgat ttttaattca attaaaacga
    35221 atttaaattc attctacata ttgagagggg ttgaggctga gctttacaaa cggtttcttt
    35281 ttaagcaact catcttcaac catgcataaa gcgggtgcat tcgctgccgc ataccattat
    35341 tcttgatctg acggaagtct ttttgtaaca attcaaactt ctttgatgta aacaaattaa
    35401 tacaacaaac ggaattgcaa acttacacac gcatcactgc gtagatcaaa aaaacaatca
    35461 ccgcacgagg tttcatgatg gaaggtcaac agcacggcga gcagctaaag cgcggcctta
    35521 aaaaccgcca tattcagctt atcgcgctgg gtggcgcgat agggacaggg ctattcctgg
    35581 gtagcgcctc cgtaatacag tccgcagggc cagggattat cctgggttac gccattgctg
    35641 gttttatcgc ctttctgatc atgcgtcagc tgggtgaaat ggtggtcgaa gaacctgtcg
    35701 caggctcctt tagccacttt gcttataaat actggggcag ttttgccggt ttcgcctctg
    35761 gctggaacta ctgggtactg tacgttttag ttgccatggc tgagctgact gccgtgggta
    35821 aatacattca gttctggtat ccggaaatcc ccacctgggt ttctgccgcc gtattctttg
    35881 tggtgattaa cgccatcaac ctgaccaacg ttaaagtgtt tggcgagatg gagttctggt
    35941 ttgccattat caaagttatc gcggtggtag cgatgatcat cttcggcggc tggctgctat
    36001 tcagtggcaa cggcggcccg caggcgaccg ttagcaacct gtgggatcag ggtggtttcc
    36061 tgccgcacgg cttcaccggg ctggtgatga tgatggcgat tatcatgttc tcgttcggtg
    36121 gtctggaact ggtggggatc accgcagcag aagctgataa cccggagcaa agtataccga
    36181 aagcaactaa ccaggttatc taccgcatcc tgattttcta tattggttcg ttagccgttc
    36241 tgctctcact gatgccgtgg acccgcgtta ccgccgatac cagtccgttt gtgctgatct
    36301 tccacgagtt aggcgatacc tttgtggcga atgcgctgaa catcgtggta ctgactgcgg
    36361 cgctctccgt gtacaacagc tgcgtatatt gcaacagccg tatgctgttt ggtctggcac
    36421 aacagggtaa tgcgccaaaa gcgctggcgt ctgtcgataa acgtggtgta ccagtaaata
    36481 ccattctggt gtctgcactg gtaacggcgt tgtgcgtact gattaactac cttgccccag
    36541 agtccgcttt cggactgtta atggcgctgg tggtatctgc actggtaatc aactgggcga
    36601 tgattagcct ggcgcatatg aaattccgtc gcgccaagca ggaacaaggc gtggtaactc
    36661 gcttccctgc tctgctttat ccgctgggta actggatctg cctgctgttt atggcggcgg
    36721 tactggtgat tatgctgatg accccaggaa tggcgatttc ggtatacctg atcccggtat
    36781 ggctgatcgt gttaggtatc ggctatctgt ttaaagagaa aaccgccaaa gccgtaaaag
    36841 cgcattaatc tctctacgcc ctcacccgta cagggtgagg gcaataatct ttacaccaac
    36901 gccccgtaaa tcgtcagtag tgcaatcacc accacgacca cgaacgaggt tttcttcgcc
    36961 attgaaaccg ctgccttcgg cgtctccacc ttatcgacat gcggttcacg cgccagagag
    37021 aactgcgcca gacgcgttaa cacctgatac tgcgaagtat ggaaatcacc cagcgaagca
    37081 aaccaggccg gtaacgcttt ctcaccatga ccgatcaagg catataccac acccgcaaga
    37141 cgaaccggca cccaatccag tacatgaagc acggcatcaa tgccggactg taaacgatga
    37201 tgcggcgtct gatatcgcgc cagccagtat tgccatgcac gcaaaaacgc ataccccatc
    37261 agcgtaacgg gtccccaggt tccccccaca atcagccaga acagcggtgc aagataaaaa
    37321 cgaaagttaa tccacagcaa tgcattttgc agctcacgca aatactcacg ttcgtcgcag
    37381 cctgccggga cgccgtgaat catggtgagt tcgccagcca tcgtggcacg ggcatggcta
    37441 tcattacgtg aagcagctgt cagataagca tgataatgaa gacgaacttt acctgcgcca
    37501 atacacagca aaccaatcag cagccacacc agtagcgtgg gaacgttgaa caatactccc
    37561 tgcaatgcgc gtaacagtaa aaaagtcacg cccatcgcaa taatggtcat gcctaacgtg
    37621 cgcccgagag aaaaatgttt cacccgccga aagaacgctt caagacgatg atcaagctgc
    37681 cagtgctcgc ccaacttaaa caggcgctcg aaaattaaca ccagtaaggt tgtaaatagc
    37741 gtcatgttgt ctccttgctg accagcacac gaaaccgtgc ccaatcaaat gcaggaccgg
    37801 gatcggtttt ccgatccggc gcaatatcac aatggcccgt catgttttta gcgatatccg
    37861 gatagcaatc aatcagtgcc cgcgtaaccg ccgcaagctg ttgatactgc gcatcggtat
    37921 acgccagcgt atcggtgcct tcaagctcaa tcccaataga aaaatcattg cagcgttcgc
    37981 gcccctgata ctgagagact cccgcatgcc atgcacgttt atcgaaagga acatactgga
    38041 ctatttcacc atcacggcga atcaaacagt gagcggagac gcgcaaatgg gcgatctcag
    38101 caaagaaagg atgtgcctgc ggatcaatag ttccagtgaa taatgcgtcg atccacggac
    38161 cgccaaactc gcctggcggc aggctaatat tgtgcaccac cagcagggtg ggtgtttcgt
    38221 catccgggcg gcaatcgtaa tgtggtgagg gaacgcggcg cgcgccaacc agccacccct
    38281 gttctaacaa catgcaggat ctccttatat gtggtgctaa tacccggttc agagtagcat
    38341 gtttctacct tatgattcgt tagctatctg gagttttaac atgccgcctc gccgctataa
    38401 ccctgacacc cgacgtgacg agctgctgga acgcattaat ctcgatatcc ccggcgcggt
    38461 ggcccaggcg ctgcgggaag atttaggcgg aacagtcgat gccaacaatg atattacggc
    38521 aaaactttta ccggaaaatt ctcgctctca tgccacggtg atcacccgcg agaatggcgt
    38581 cttttgcggc aaacgctggg ttgaagaggt gtttattcaa ctggcaggcg acgatgtcac
    38641 cataatctgg catgtggatg acggcgatgt catcaatgcc aatcaatcct tgttcgaact
    38701 tgaaggccca tcccgcgtgc tgttaacggg cgaacgcact gcgcttaatt ttgtgcaaac
    38761 cctttcagga gttgccagta aggtacgcca ctatgtcgaa ttgctggaag gcaccaacac
    38821 gcagttgttg gatacgcgca aaaccttacc cggcctgcgt tcagctctga aatacgcggt
    38881 actttgcggc ggcggagcga atcaccgtct ggggctttct gatgccttcc tgatcaaaga
    38941 aaaccatatt attgcctccg gctcagtgcg ccaggcggtc gaaaaagcgt cctggctgca
    39001 cccggatgcg ccagtagaag tcgaagtaga gaatctggaa gaacttgatg aagccctgaa
    39061 agcaggagcc gatatcatca tgctggataa cttcgaaaca gaacagatgc gcgaagccgt
    39121 caaacgcacc aacggcaagg cgctactgga agtgtctggc aacgtcactg acaaaacact
    39181 gcgtgaattt gccgaaacgg gcgtggactt tatctccgtc ggtgcgctaa ctaaacacgt
    39241 acaagcactc gacctttcaa tgcgttttcg ctaatagttg acagatttat ccgctccatc
    39301 gcgggcggat aatctcccct tccccaactt tcttcgtaac gcctcgcaaa tttcttgtct
    39361 ttcgctgcaa cgaaataaaa aacctcggaa tctgctttcc cgtttcgttt tttgcccctc
    39421 gcctgtgctg atggcgtttg ccaaagtagc gccaaccaaa tcaaggagcg aaacagatgg
    39481 acaagcaacg cggttttaca cttatcgaac tgatggtggt tattggcatc attgccattt
    39541 taagcgccat tggtattccc gcttatcaaa actacctgcg caaagccgca ctcaccgaca
    39601 tgctacaaac ctttgtgcct taccgtaccg ccgtagagtt gtgcgcgctg gaacatggtg
    39661 gattagatac ctgcgacggt ggcagcaatg gcattccctc gcctaccacc acccactatg
    39721 tttcagccat gagtgtggca aaaggcgtgg tgtcgctgac cgggcaagaa agtctcaatg
    39781 gactaagcgt cgtcatgaca ccgggttggg ataacgcaaa cggcgtcacc ggctggacgc
    39841 gcaactgcaa tattcaaagt gacagcgcat tgcatcaagc ctgcgaagat gtcttccgct
    39901 ttgatgacgc caactaagga gcggcaatga atattccaca gctcactgcc ctgtgtctgc
    39961 gttatcatgg agtcttgctg gatgccagcg aagaggtggt tcatgttgcg gtagtcgatg
    40021 caccttcgca tgagctactg gacgcattgc atttcgctac caccaaacgt attgagatca
    40081 cctgctggac gcgccaacaa atggaaggtc acgccagtcg cacacaacag acattgcccg
    40141 tagctgttca ggagaagcat cagcccaaag cagagttgct aactcgaacg ttacaatctg
    40201 cgctggaaca acgcgcgtct gatattcata tcgaaccagc ggacaatgcc taccgcatcc
    40261 gcttgcgtat cgacggcgta ttgcatcctt taccggatgt ttcaccggat gccggagtcg
    40321 cattaaccgc cagattaaaa gtgctgggaa acctggatat tgcggaacat cgcctgccgc
    40381 aggacgggca attcactgtc gaactggcag gaaacgccgt ctcatttcgt attgcgacct
    40441 taccatgtcg gggtggtgaa aaggtggtat taaggttgtt acagcaggtg agccaggcac
    40501 tggatgtcaa cacgcttgga atgcagccgt tacaactggc ggactttgct catgccttgc
    40561 aacaaccaca gggactggtg ctggtaactg gccctacagg cagcggcaaa acggtcacgc
    40621 tttatagtgc cctgcaaacg ctgaataccg ctgacattaa tatttgtagc gtcgaagatc
    40681 cggttgagat ccccatagcc ggactaaacc agacgcaaat ccatccgcgt gccggactca
    40741 cctttcaggg cgtgttgcgt gcgttattgc gccaggatcc tgacgtcatc atgatcggag
    40801 agatccgcga tggcgaaaca gcagagatcg ctattaaagc ggcgcaaact ggtcacctgg
    40861 tgttgtctac cctacacact aattccacct gcgaaacgct ggtacgttta cagcaaatgg
    40921 gggtcgcccg ctggatgcta tcatcggcgc ttacgctggt aatagcccag cgtctggtac
    40981 gcaaactttg cccacattgt cgccggcagc aaggggagcc catccacatt ccagacaatg
    41041 tatggccatc gccgctgccc cactggcagg cacccggttg tgtacattgc taccacggtt
    41101 tttatggtcg tacggcctta tttgaagttc tgcccataac gccggtcatt cgtcagctta
    41161 tttccgctaa taccgacgtt gaatcgctgg aaacgcacgc acgacaggcg ggtatgcgta
    41221 cgctttttga aaacggctgc ctggccgtgg agcaaggctt aaccaccttt gaagagttaa
    41281 tccgcgtact ggggatgccg catggcgagt aagcaactct ggcgctggca tggcataacc
    41341 ggcgacggca atgcgcaaga tgggatgcta tgggcagaga gccgtgcttt gctgctcatg
    41401 gcactacagc aacagatggt taccccactt agcctgaagc gaatcgccat caattctgcg
    41461 cagtggcgag gagataaaag cgcggaagtc attcatcaac tggcgacgct actcaaagcc
    41521 gggttaacgc tttctgaagg gctggcactg ctggcggaac agcatcccag taagcaatgg
    41581 caagcgttgc tgcaatcgct ggcgcacgat ctcgaacagg gcattgcttt ttccaatgcc
    41641 ttattaccct ggtcagaggt atttccgcca ctctatcagg caatgatccg cacgggtgaa
    41701 ctgaccggta agctggatga atgctgcttt gaactggcgc gtcagcaaaa agcccagcgt
    41761 cagttgaccg acaaagtgaa atcagcgtta cgttatccca tcatcatttt agcgatggca
    41821 atcatggtgg ttgtggcaat gctgcatttt gttctgccgg agtttgccgc tatctataag
    41881 accttcaaca ccccactacc ggcactaacg caggggatca tgacgctggc agactttagt
    41941 ggcgaatgga gctggctgct ggtgttgttc ggctttctgc tggcgatagc caataagttg
    42001 ctgatgcgcc gaccgacctg gcttatagtg cggcagaaat tgctgttacg catcccgatt
    42061 atgggttcac tgatgcgggg acaaaaactc acgcagattt ttacgattct ggcgctgaca
    42121 caaagtgcag gcattacttt tttgcagggc gtagagagcg tcagagaaac aatgcgctgc
    42181 ccgtactggg tgcaacttct gacacaaatc cagcacgata tcagtaacgg tcatcccatc
    42241 tggctggcgc taaaaaatac cggggagttt agcccgctct gtttgcaatt agtgagaaca
    42301 ggagaggcat ccggctcgct ggacctcatg ttagacaacc tcgcccatca tcatcgggat
    42361 aacacaatgg cgctggcgga taacctcgca gccttactgg aaccggcgtt gctgatcata
    42421 acgggaggaa ttatcggtac gctggtggtg gcgatgtatc tgccaatttt ccatttaggc
    42481 gatgcgatga gtgggatggg ataacgctgg cgtgttgctc cacaccagcg ttggaagatt
    42541 acaggttgtt gaagatgcgg ttttcttgtt cctgcacacg gataaatgtg gtgcgcttgg
    42601 tcagttcttt caggcgtgaa gccccaacgt atgtacaagc tgaacgcagg ccgcccaaaa
    42661 tatcacgcgc ggtattttca accgggcctc gcagcggcag cttaacggtt ttaccttctg
    42721 ctgcgcgata ttccgcaacg ccgccaacgt gacgtttcat cgcagactcg gagctcatgc
    42781 cgtagaacag cataaatttc tcgccgttct cctcaacgat gcgaccgccg ctctcttcgt
    42841 ggcccgccag catgccgcca agcatgacga aatcggcacc gccgccaaag gcttttgcga
    42901 catcgcccgg cgtggtgcag ccaccgtcgc tgacgatcat tccgcccaga ccgtgcgccg
    42961 catcggcaca ttcgattacc gcagaaagct gcggataacc gacgcctgtt ttgacgcgag
    43021 ttgtacaaac agaacctggg ccaatgccaa ctttaacgat atcagcaccg gagaggataa
    43081 gctcctcaca catttcacca gtcactacgt tgccagcaca aatggttttg gtcggccacg
    43141 cttcacgcgc tttcgcaacg aactgcacga agtgttcgga ataaccattc gccacatcaa
    43201 tacaaacgaa gtttaatgcc gggttcaggt cgagaatctg tttagttttt tcgaaatccg
    43261 catcagacgt accggtagaa accatcacat gtttcagcac atcagcggaa gaattgttga
    43321 taaacgcttg ccactcttcg acagaatagt gtttatgcac agcagtcaaa atatcaaaag
    43381 aagccagcgc agaggccata gaaaatgtac ctacggtgtc catatttgcg gcgataattg
    43441 gcacgccgga ccagctctga cctgaatgtt tgaaggtgaa ttgacgttcc agttcaacat
    43501 cggaacggct tttaagagtg gagcgtttag ggcggatgag aacgtcttta aaacctaact
    43561 tcagatcttc ttcaatacgc atgtgcggat tcctggggtt aatggctata agggtaagag
    43621 cgcaactcca gtgacgctat catacgcact aatcaatgcg gcgcaagact gcgaaattgt
    43681 ctttttttac gctacaatcc cataaattta cctggtaaat agtgagcgaa cgtttcatca
    43741 atttcgcttt cgtgcactca tttttaacgt tttgataatc atgatgaatt cccgggaata
    43801 taaattatga ggtatatagt tgccttaacg ggaggcattg gcagtggcaa gagtaccgtt
    43861 gccaatgcgt ttgctgatct cggaattaac gtcattgatg ccgatattat tgcgcgtcag
    43921 gtggttgaac caggtgcacc tgcgctacat gccattgctg atcactttgg cgctaacatg
    43981 attgctgctg atggaacatt gcagcgccgg gccttgcgcg agcggatctt cgccaacccg
    44041 gaagagaaaa actggcttaa cgccctgctg catccgctga ttcagcaaga gacgcaacac
    44101 cagatccagc aagctacttc cccctatgta ctgtgggttg tgccattgct ggtagaaaac
    44161 tcactgtata aaaaagcgaa tcgagtgctg gtggtggatg tcagcccaga aacgcaactt
    44221 aagcgcacca tgcagcgcga tgatgtaact cgcgagcatg tcgaacaaat ccttgctgct
    44281 caggcaacgc gcgaagcccg ccttgccgtg gcagatgacg tcattgataa taacggcgca
    44341 ccggatgcta tcgcatcgga tgttgcccgc ctgcacgcac actatttgca gcttgcgtcg
    44401 cagtttgtct cacaggaaaa accgtaatgc agacccaggt cctttttgaa catccactaa
    44461 atgaaaaaat gcgtacatgg ctgcgcattg agtttttgat tcagcaactc accgttaatt
    44521 tacccatcgt tgaccacgct ggcgcgctgc atttcttccg taatgtcagt gaattactgg
    44581 atgttttcga gcgcggcgaa gtccgcactg agctgttgaa agaacttgac cggcagcaac
    44641 gtaaactcca gacctggatt ggcgtgcctg gcgtggacca gagccgtatt gaagcattaa
    44701 ttcagcagtt aaaagcggcg gggagcgtat taatttccgc gccgcgtatc gggcaatttc
    44761 tgcgtgaaga tcgtttgatt gctctggtgc gtcagcgact gagcatccca ggcggctgtt
    44821 gcagctttga tttacctaca ttgcacattt ggctgcatct accccaggcg cagcgcgaca
    44881 gccaggtaga aacctggatt gccagcctga acccgctcac ccaggcactt accatggtgc
    44941 tggatttaat tcgccagtcg gctcccttcc gtaaacaaac cagcctgaat ggtttttatc
    45001 aggataacgg tggcgatgcc gacttgctgc gcctgaatct gtcgctcgat tcacagcttt
    45061 atccgcaaat ttccggacat aagagccgtt ttgccattcg ttttatgccg ctggacactg
    45121 aaaacggaca ggtaccggaa cgtctggatt tcgaactggc ctgttgctaa ggagtgaaaa
    45181 tgtcagaaac tattacggtg aattgcccaa cctgcgggaa aacggtggtg tggggtgaaa
    45241 tcagcccgtt tcggccattt tgctccaaac gttgtcagct gatcgacctc ggagaatggg
    45301 ctgctgaaga aaaacgaatc cccagcagtg gcgatctttc cgaaagcgat gactggagcg
    45361 aagagccaaa gcagtgacat ttgtctgatg ccgcacgtag gcctgataag acgcggacag
    45421 cgtcgcatca ggcatcttgt gcaaatgtcg gatgcggcgt gaacgcctta tccgacctac
    45481 agacgcttaa gcttcgcaat taccggttca ttggctggcg gaaaatcatc ggcattaaga
    45541 ccgactagcg acatccactc accgggttgc ccttctttac cccacggctc cccttcccag
    45601 ctttcgacca gccagaacca cagagttata tgcctgtccg ggaattcata ttccagtttt
    45661 tcaaatagcg aaaaatgttg tggggtaatc ccgacttctt cctgaagttc acgcaccacc
    45721 gcctgttccg gcgtttcgcc catttcaatt ttaccgccgg gaaactccag tttattcgcc
    45781 atgtgcgcat ctgctgcgcg acgcgttata aagatttcat tgttctcgtt gcgaataata
    45841 cctaccgcaa tttgcagctt tttcattgtc ttaaacctat aaaaaaggcg cagaatcctg
    45901 cgccttttac ttcaactgtt agcttttatt gcaggcgacc atggcactgc ttgtattttt
    45961 taccagaacc gcacgggcaa ggatcgttac gtcctacttt gcgctcaccg gtttgcgccg
    46021 ccagtgcagc tgcggctgca gagtcgtcat cctgatggct aagctgctgc atttgcgcta
    46081 aacgctcggc ttccatacga cgctgttgtt ccagctcctc aacctcttca ggcatacgta
    46141 cctggacttt gctcagcgtg ctgataactt catattttaa cgactccagc atcgctgcaa
    46201 acatggagaa cgattcacgt ttgtattcct gcttcggatc tttctgtgca tagccacgca
    46261 ggtggatacc ctgacgcaga tagtccatcg ctgccaggtg ctctttccac agggagtcaa
    46321 gcgtttgcag catgacgcct ttctcgaagt gacgcatcat ctcagcacca accacttctt
    46381 ctttacgctg atacacttcg atggactgcg ccagaatgcg ctcacgcagc gtctcttcat
    46441 gcagttctgg ttctttatcc agccactcgg caattggtaa atcgaggtcg aaatcgttct
    46501 tcagacgttc ctgcagcccc ggaatatccc acatttcttc cagcgactgt ggcggaatgt
    46561 aggcatcaat ggtggctttg aacacatctt cacgaatgct gttgatggtt tcgctcacat
    46621 cgctgacatc cagcagttcg ttacgctggg agtaaatggc gcgacgctga tcgttagcca
    46681 cgtcatcata ttccagcagt tgcttacgaa tgtcgaagtt acgactttca actttacgct
    46741 gggcgttggc aatcgcttta gtcacccacg ggtgttcaat ggcttcgcct ggcttcatac
    46801 ccagtttacg catcatgccg gatactcggt cggaagcaaa aatacgcatc agcgcatctt
    46861 ccatcgacag gtagaaacgg gaagaaccag catccccctg acgaccagaa cgaccgcgca
    46921 actggttatc gatacgacgg gattcgtgac gctcggtacc gatgatatgc aggccacctg
    46981 cttccagtac cgcatcgtga cgtacctgcc agtcggcttt aattttttca atttgctcta
    47041 cggtcggatt ttccagcgcg gcaacttctg cctgccagct accaccgagc acaatatctg
    47101 taccacgacc cgccatattg gtcgcgatag tcaccgcagc cggataacct gcctgagcaa
    47161 caatcgccgc ttcgttggcg tggaatttgg cgttcaggac gttgtgctta ataccggctt
    47221 tggtcagttc gtttgacacc agctccgatt tttcgatgga gatagtaccc accagcaccg
    47281 gctggccttt cgcagtacgt tctttgatat cttcaatgat cgcctgaatt ttttccgctt
    47341 cagtcatgta gaccaggtcc ggcagatctt tacgaatcat tggacggttg gtcggaacaa
    47401 cgacggtatc cagcttgtag atggagctaa attcgaaagc ttcggtatca gcagtaccgg
    47461 tcatccccgc cagtttttca tacagacgga agtagttctg gaaggtgatc gaagccagcg
    47521 tttggttttc gttctggatc tgcacacctt ctttcgcttc cacagcctgg tgcagaccat
    47581 cggaccagcg acggccctgc atggtacgac cggtgtgttc gtcaacgatg ataacttcac
    47641 catctttaac gatgtagtcg acgtcgcggg taaacagcgc atgagcgcgc agcgccgccg
    47701 ttacgtggtg catcagcatg atgttggccg gagagtacag agactcccct tcatccatga
    47761 tgccctcttt caccagcagt tcttcaatca gcaccagacc acgttcggtc aggttcacct
    47821 ggcgagattt ttcgtccacc gagaagtggc cttcgccctg gaaggtttcg gagtcttctt
    47881 tttcctgacg gatcaggtgc ggaataattt tattcacgcg tttatacatt tccgagctgt
    47941 cttctgccgg gccggaaatg atcagcggtg tacgcgcttc atcgatcagg atggagtcca
    48001 cttcgtccac cagcgcatag tgcagtttac gctgtacacg ttcttcaggg ctgaacgcca
    48061 tattgtcgcg caggtagtca aagccgtatt cgttgttcgt accgtaagtg atgtcagctg
    48121 cgtaagcttc gcgctttgcc ggtgctggca tgcccggcag gttgataccg acagtcaggc
    48181 caaggaattc aaacagcgga cggttgtttt cggcgtcacg ttgcgccagg tagtcgttga
    48241 cggtaactac gtgcacgcct ttaccggtta gtgcgttcag gtaagcaggc agcgttgcgg
    48301 tcagggtttt accttcaccg gtacgcattt cggcgatgca gcgttcgtta agaaccatac
    48361 cgccgagtaa ctgaacgtcg aagtgacgca taccaaagac gcgcttactt gcctcacgta
    48421 ccacggcgaa agcttccggg atcagatttt ccagcacttc gcctttttcc agacgtgcac
    48481 gaaactctgc ggttttccct ttcagttctt cgtcggagag tttttccatc tccggttcca
    48541 tggcattgat gatgttgacc actttgcgca tccggcgcag ggtgcgatcg ttacgactac
    48601 cgaaaacttt agttaacaat ttgattagca taataaaatc tcaaacgccc cgcgttgcgg
    48661 agttaataaa atgaagtaaa ggtttattgt tgttaggtga ggcgttgagg gccagcacgg
    48721 atgccttgcg cctggcttat ccagacgggc gtgctgaatt ttgcttgtgg ggtaaaatgc
    48781 gcataatcaa tgcgataacc cttttcagac ggcgtgcctt cctgggtcag cagcgcgctg
    48841 agcgtatcca gtaatgcaag atgttgcgcc tgaagaggca aagattcttc agcaacgggc
    48901 agtgtttgcg gtgccattgc gaaagaaaga tgacggatta ccgtgcgaat ggcatgttga
    48961 tgccagtaat caacggaata gttcgaattc gggcggcgtg tgttcgcttc cagcaaggcc
    49021 aattgaccaa agttaacttt ggctgaaggc tcgtggttgc gggttgtcgc ttttgcgggc
    49081 gcgtttggtt cggcggcgtt gctgagcgca ggcaaaccta aactcgccgc aaccatccct
    49141 aataagagat gcggccagaa gtagcgttta ccaaactgtc gccagcgcgt cagtattcca
    49201 ctcacgttat tgccatcccg atcgtattca aacgtattta gcgcacaaat cttatcatta
    49261 aagcctgcag tccacagcac gaatgaagat aagttgtgcg gaaaagtgct taagaaaaca
    49321 gcgttcgcac cgttttttat agctcttata aaatttaaaa aaaaacgact ggttcacctg
    49381 gccggagaga gtgccagatt tgccagtcga attttatacg acagtataaa tgtcgttatg
    49441 ccagtacagc tgaaggcgct ttgaaggcca acggcagttc tgcgtcgtcc tggaaggtca
    49501 catattccca ggcttcctgt ttcgccagga cagcctgcag cagtttgtta ttcagtgcat
    49561 gaccggattt ataagcggta aatgcaccaa taatattgtg accacacatg aacaagtcac
    49621 cgatcgcatc gagcattttg tgacgcacaa attcgtcttc aaaacgcagg ccgtcttcgt
    49681 tcagtacgcg ataatcgtca acaacgatgg cacaatcgaa gctgccgccc aggcacaaac
    49741 cacgggactg cagatattcg atatcacgca tgaaaccgaa cgtacgcgca cggctgatct
    49801 ggcgcataaa cgcatcagcg gagaagttca tcgcatagcg ctggttgctg gaatcaatag
    49861 ccggatggtt aaaatcgatg gtgaaatcca gcgaaaaacc attgtacggc ttaaattcag
    49921 cccacttatc gccatcttcg acacgaacag tctctttgat gcgaacaaat tttttggcgc
    49981 agttcaactc gtcgataccg gcgtcaagca gcaggtatac aaacggagcg gcgctgccgt
    50041 ccatgatcgg gatttccggc gcgttaactt cgataacaat gttatcgatg cccaagcccg
    50101 cgagagcagc attgaggtgc tctacggttg aaatccgtac atcatgctcg ttgaccagac
    50161 acgtacagag catggtatca cgcacagatt tggcatcggc cgggaaatct accggtggat
    50221 tcaagtcggt gcgacgatag atgaccccgg tgttggccgg cgcagggcgt aacgtcaggg
    50281 tgactttctt gccggtatgt aaaccgacac ccgtcgcctg aacgatacgt ttaagtgtcc
    50341 tttgtttgat catcgtatta tctcgccaaa ttacctatcc aactgaagtg tactatacat
    50401 tcggcgggcc agtttagcac aaagagcctc gaaacccaaa ttccagtcaa ttcttaatca
    50461 gcttgcttac gcaggaatgc tgggatatcc agataatccg gctctttcgc agtttgcggc
    50521 gcattgtcat tcacgacttt agcaaccggc ttctgctcct gggtcagcgg agccatccca
    50581 tgctgctggt agcgatccat cactggctgc tgaacctgct tattggtcac cagagtgatt
    50641 tcaggacgtt tgtccatgcc gatacctgtc gcaacaacgg ttacgcgcag ctcgtcattc
    50701 atatccgggt caagagaagt accgataacc acagtcgcgt tgtcggaagc aaatgcacgg
    50761 atggtgttac ctaccgtttc gaactcatcc agacgcaggt cgaagcccgc cgtgatgtta
    50821 accagcacgc cgcgcgcgcc agacaggtcg atatcttcca gcagcggaga agagatagcc
    50881 atttcagcag cttcttccgc acggtcttca ccgctcgcca cgccagaacc catcattgcg
    50941 tagcccatct cagacattac ggtgcgtacg tctgcaaagt ccacgttcat caaacccgga
    51001 cgagtaatca gttcagcgat accttgcaca gcgcctttca gtacatcgtt cgctgcgcca
    51061 aacgcatcca gcagggagat accgcggccc agaactttca gcagtttgtc gttcgggata
    51121 gtgatcagag agtccacatg cttggacagt tcagtgatcc cctgctccgc gaatgccata
    51181 cgcttcttgc cttcaaagtt gaaaggctta gtgacgacag caacggtcag gatacccaaa
    51241 tcttttgcca cttcagcgac gactggtgct gcacctgtac cggtaccacc ccccataccc
    51301 gcagcaataa agaccatgtc tgcaccttcc agcgccgcac gcaatgcatc gcgatcctca
    51361 tcagccgcat tgcggccaac ttctggatta gcgccagcgc ccagtccttt ggtgataccg
    51421 ctaccgattt gaatcgtctg tccaaccgct gttttacgca gcgcttgtgc atcggtattt
    51481 accgcgaaga attcaacacc ttcaatgcgc tcgcgcacca tgtgttcaac agcattaccg
    51541 ccgccgccgc cgacgccgat gactttaatc accgcgtcat tggtaagttc cattggttca
    51601 aacatagttt ctctccgatt tgtgcctgtc gcctgaggcc gtaatcatcg tcggcctcat
    51661 aaaaattaaa actcttttcg cagccaacta ttgagtcgct tgatccacga gccaactgat
    51721 gctgtaacac gtttttctac ttcagcttca ccgttaagat gtgactcttt cccatagtga
    51781 agcaatccca ccgccgtcga ataatacggc tcctgagcat aatccgttaa accggtaatg
    51841 ttcagcggcg cgccgatacg cacttgcgta tgaaacacgc gctgagcaca ggctgcaaga
    51901 ccttcgatct gcgctgcgcc accggttaat acaatgcctg ccgccaggtg atgtttaacc
    51961 ccttgttggc gaagcttttc ctgcaactgc aatatctctt cgttgaccag gttgagcagc
    52021 tcggtatagc gcggctcgat cacctctgcc agggtctgac gttgcagact ccgcggcgga
    52081 cgaccaccta cgctcggcac ttccacgctc tcatcttttc caacgatgga acccagcgca
    52141 caaccgtggc gaactttaat cgcttcggcg tcgcttggcg gcgtgccaaa ggcgtaagcg
    52201 atatcactgg tcacgacatt gccagcataa ggaattacct tagtgtggcg caatgcccca
    52261 ccggtataaa cggcgatatc cattgtacca ccaccgatat cgacgacgca gacacccagt
    52321 tcacgttcat cttccgtcaa taccgaataa cttgatgcca gtccggcaaa tatcagttgg
    52381 tcaactttca gcccacaacg ttcaaccgct ttgacgatgt ttttcgccat atcgttgtga
    52441 catgtgatca ggtgcacttt tgcctgcatc cgcacgcccg aaagtcctac cggattcttg
    52501 atcccttcct gatagtcaat cgcatactct tgcgggatca catgcagcac acgatgctca
    52561 tcgcgcacac gcaccgattt cgcggtatgg acgacgtttt ccacatcttc ttgcgtcact
    52621 tcttcttcag aaataggcac cataccaatt tcattctggc agctgatgtg cttaccagaa
    52681 agcgccagat ataccgaaga gatctgacaa tctgccatca attctgcctg gtcaatggcg
    52741 cgttgtacgc acttgaccac ggattcgagg tcgttcaccc cgcctttatc cataccacgt
    52801 gacgggcagc ttcccacgcc aatgatattg accataccgt cgggcagaac ttcccctact
    52861 aaagcggcaa ccttcgcggt accaatctcc agtcctacta ccagttttct gtccgtcgcc
    52921 ttgatcattg ttgttctgcc tgtgcctgat tttgttgctg agtagattcc tctggcggca
    52981 agggcgccca gcctactgcc gctccagagt cataacgcaa atcaacgtag ctaatccgtt
    53041 tgccatcggt ttgcgcctgc tgctgtaaaa ccggataaag ttctacaaag cgagccaaac
    53101 gtttcatcgt atcgccccgg ccaagattga gcttaatatc gttattcagc gtcaactgcc
    53161 aggaacgccg cgcggtcatc gccgcttcct tcagagtaaa tctgtccttt gccagcatct
    53221 gccccatttc gcgatagccc tgcaacactt cattggcgct gccttccggg ccatacagca
    53281 ttggaagcac ctgcttgctg gtgcgatctg gcggcacgct gaaggtgttt ccttccgcgt
    53341 ctaccatatg ttgatcattc caccgcgcaa tcggcacata ttcaaccaga tgaatcttca
    53401 attcatcagg ccactgcttt ctgacgctca cctgcttaat ccacggcagg cgttgttcta
    53461 tttgcgtctg gatgatgttg acatcttggg tcataaaggt acccggctca cccaatgcca
    53521 ggatcgactg ccggatatcg tcattacgcg tgtaatggcg ttcaccggtc aacaccagct
    53581 ttgagagcgg caggcgttgc gcatcttcca tccagcccaa cacgacccag ccgctcacca
    53641 acactgtcgt taaaacggtc agcaggaaaa ggatccccgc cagacgcgtt ccattattgc
    53701 ggcgagaaga aacctcttct tcgctatttc gcgtgttcag agcagcctgc gacatattag
    53761 tccgccagtt ccagaattcg tactaccaac tgcgagaagc tcatacctgc ctgacgtgcc
    53821 gccatcggca ccaggctgtg gctggtcata cccggtgagg tattggcttc cagcagataa
    53881 aactgtccat cgctgtccag cataacgtca atacgtcccc atcctttgca acctaacgtc
    53941 gtccatgctt tcagcactaa tgcctgcaaa ttggcctctt gtgacgcttc cagacctgcg
    54001 gggcagaaat actgtgtctc atcagagaga tacttcgcct cataatcata gaaggttccg
    54061 gacggttgaa tacgtattga cggtaaaatt tcttcaccga gtatcgcaac cgtgaactcc
    54121 ggcccactta gccatttttc aatcaatact tcttcatcgt gctgaaatgc caatcttaat
    54181 gcatcttgta gagcattttc tgctactact tttgacattc ccacactgga accttcgcgg
    54241 ctcggcttaa cgataaccgg caaacccaga gcagaaattt ctgctaactg cttatcgctc
    54301 aggccttttt caaactctgc gcgggttaac gctacccacg gcgcgaccgg taaaccggca
    54361 ccttgccaca gcagtttgct gcgtagttta tccattgaaa gcgcagatgc catcactccg
    54421 ctgccggtat aaggcaagcc catcagctcg agcatgccct gcagcgtacc atcttcaccg
    54481 ccgcgaccgt gtagcgcgat aaacactttc tgaaaaccca tcgacttcag ttgcgtcacg
    54541 tcgacttctt tcgggtcgac aggatacgca tcaataccgc cttcacgcag cccggctaac
    54601 accgctgcgc cagaattcag agaaacttcc cgctcagcgg aagtcccgcc caacaggacc
    54661 gcgattttat cagtcatgtt gttcttcctc cggagtttgc ggcttcagtt tgatttcagc
    54721 taaagaacgg gcaatttttc cgatattacc agccccctga acgagaatca ggtcgttacc
    54781 ggttaatacc ggtgccagca tctcggctac ctgcgcaggg tccggcacca gaatgggatc
    54841 aattttccca cgtccacgaa ttgtgcgaca cagcgaacgg ctgtccgctc ccggaattgg
    54901 cgcttcgcca gccggataca cttccagcat caacagggta tcaacctgcg tcagcacatt
    54961 ggcgaaatca tcatacaggt cgcgcgtacg ggtaaaacgg tgcggctgaa acagcattac
    55021 caggttttta tccggccagc ctgcgcgcgc cgctttaatg gtggcgtcaa cttccgtcgg
    55081 gtggtggccg tagtcatcga ccagcattgc cgtaccgctt ttaccattca ctggctccag
    55141 cgggaattca ccgaggaaat caaaacggcg cccagtcccc tggaagctct ccagcgcacg
    55201 taaaatagcc tcgtcatcaa ttccctcttc cgtagcaacc gcaaccgcag ctgcggcgtt
    55261 cagcgcgtta tgacgacctg gcgcattcag ggtgacgcgc atcggctctt tgtcctggcg
    55321 cagcagcgta aagtgcccct gcgggccaat ctgctgataa tcttctacac gcacgtcggc
    55381 atcttcgctg aagccgtaag tcgtggtctg acgtcccact cgcggtaaca attcgcggat
    55441 caccggatca tcaacacaca tcaccgcacg accgtaaaac ggcaggttgt gcagaaaatt
    55501 aataaaagtc tgttttaaat tctcaaagtc gccctggtag gtatccatgt ggtcggcttc
    55561 gatattggtg acaatcgcca ccatcggttg cagatgcagg aacgatgcat cactctcatc
    55621 tgcttcggca atcaggtacc gaccatgccc caaacgcgca tgaacccccg ccgcttttac
    55681 cagcccgccg ttaacgaagg ttgggtcgag ccccgcttct gcgtagatgc tggaaaccat
    55741 cgcggtggtt gtcgttttgc cgtgcgttcc tgcaatggcg atgccatgac gaaaacgcat
    55801 taactcagcc agcatttcgg cacgacggat caccggaata cgcgcttcat gagctgcgac
    55861 aatttccggg ttatcggcag aaatggcgct ggaaacaacg accacgctgg catcacgtac
    55921 gttttccggg cgatggttga aataaatcgt cgctcccaga ttcattaact gctgcgtgac
    55981 cggatttggc gctaaatcgg aaccactgat ctgataacct tcattggcca gaacttcggc
    56041 aataccgccc ataccggcac caccgatgcc gacaaaatgt atgtgccgaa cgcgacgcat
    56101 ttcgggcacg atggaacgca gttttgccaa ttgttgtgta ttcattcttt acgccattaa
    56161 cttcttaaat tcatacgatg caaaaggcat cgctacaatt acgcccgggc agcccggctc
    56221 acttcatttg ccactcgctc ggtggcatcc ggaatggatg cagcgcgggc gcgttctgcc
    56281 atggttaata aggtttctcg cgaccacccg gccagggtgt tggcgacagc atccacgcta
    56341 agctgtggct gctcgataat tttggctgcg cccgcttttt ccagcggtag cgcattccag
    56401 tattgctggc ggtctttatg ttgaaacggc acaaacaacg ccggtagtcc tgccgcggcg
    56461 atttcactca ccgttaaagc accggagcgg caaacgacga catccgccca cgcatacgcc
    56521 gccgccatat catcaataaa ttccgtcact ttatgctgcg gttgccccgc ttcggcatac
    56581 gcctgttcaa cggattgttg cgaaccttta ccgctctgat gccagatagt gactgaatca
    56641 cccagtttcg cagcaacctg cggcattgtc tggttaagaa tgcgtgcgcc ctgggaacca
    56701 cccactacca gcacacgaac cggaccttca cgtccagcca aacgttgctg cggcaacggc
    56761 agcgccagta catcggtacg caccgggtta cccaccactt ccgcattagg gaaagcgcct
    56821 ggaaacgcct gcatcacttt ggtggcaatc ttcgccagcc atttattggt taagcccgca
    56881 ataccgtttt gttcatgaag tacaaccgga atgcctaacg accacgcggc cagaccacct
    56941 ggacctgata catagccgcc cataccgagt accacgtcag gtttgtacgc tttcataatc
    57001 gcccgcgcct gacgccaggc gttgaagata cgcagcggcg cagctatcag tgcttttata
    57061 ccttttccac gcagaccaga gatacgaatg aaatcaattt cgatgccatg ttttggcact
    57121 aagtccgctt ccatacggtc ggcagtcccc agccagcgaa cttgccaacc ctgagccatt
    57181 agatggtgcg caaccgccag tcccgggaat acatgtccac cggttccgcc tgccatcacc
    57241 attaatcgct ttccttgacc actcatcgtg aacctcgtac aaacgcctgc gctttctcca
    57301 gacgcgtttc ataatcaata cgcaacagca tcatgatggc tgtcgacata atcagtaagc
    57361 tcgaaccacc gtaactgatc agcggcaatg tcagaccttt ggtcggtaac atccccgccg
    57421 ccgcgcctac gttaaccagc gcctggaagc taaaccagat gccaatagaa caggcgagaa
    57481 aaccggaaaa acggtggtca atttctaacg ctttgcggcc aatcgacatc gcgcgaaaag
    57541 cgacgaagaa taccattaaa agtgccagca ccacaccgac ataccccagt tcttcgccga
    57601 taatggcgaa aataaagtca gtgtgcgctt ccggcagata ctccagtttt tgtaccgagt
    57661 tacctaaacc ttgtccccaa agttcgccgc gaccaaacgc catcagcgat tgcgttaact
    57721 gatagccgct gccaaaggga tcttcccacg ggttccagaa tgcggtaaca cggcggatac
    57781 ggtacggttc ggcgagtatc agcaacacaa ccgctgaaat gcccataccg ataatggcaa
    57841 tgaactgcca caatttcgct cccgccagga acaacatcgc cagcgtagtc acaaacaaca
    57901 ccaccaccgt accaaggtct ggctgtgcca gcagtaacac tgccaacacc agaatcacgc
    57961 ccatcggttt caggaagccg cgcaggttat tacgtacttc gtcgccttta cgcaccagat
    58021 agttggcgat ataacaaaac agcgacagtt ttgtcagctc cgcaggctgg atacgcagca
    58081 aaccgagatc gatccaacgc gatgcccctt taaccgagct acccactacc agcacgatca
    58141 tcagcaggat gatagatccg agcagcatcg tggcactgta gcgttgccag aactccatcg
    58201 gcagacgcag cgtaatgatc gccagaatga atgccaggat cagatagacg ccatcacgct
    58261 tcgcgaagaa gaacggatcg ttggttaagc gttgccctat gggcattgac gccgaggtca
    58321 ccatgataaa gccaatcgcc gcaaggccga aggtcagcca cagtaaggtg cgatcgtaca
    58381 tgatcaggct gtcggtatct ttttcccgcg agcccatcac ccagcctttt agcgccgtgg
    58441 agatccagac caggatactg aatcctggca ggcgcggcat tttcaggcga gggagagata
    58501 aacgcatcaa cctaactcct tcgccagacg ggcaaactca ttgcctcgtt gttcaaagtt
    58561 cttgaactga tcaaggctgg cacaggccgg ggagagcaga accatatcgc ccggctgaac
    58621 acgcggagcc agcaagcgca tcgcctgttc catagtttcg gtttgttctg ccacttccgg
    58681 gcgtagcgcc gccagctgcg caccgtcacg accaaaacag tacaaacgca cgttgtcgcc
    58741 attcagatag cgcgccagtg gactgaagtc cgccgattta ccatcgccgc ccagcaacaa
    58801 atgcagcgtg ccgtctacct gcaagccatt caacgccgct tccgtactgc cgacgttagt
    58861 ggctttcgaa tcgttaatcc agcgtacgcc attgtgctcc agcaccactt caaagcgatg
    58921 cggcagaccg gtgaaggtgg ttaacgcttt caggctgctg gcgcgcggca aaccagctgc
    58981 gtctgccagt gccagtgctg ccagcgcatt ggtgtagtta tgctgcccgg aaagtttcat
    59041 ctctttcacg ttcagcactt tctcgccttt cactcgcagc caggtttctc cctgctgatg
    59101 attcaggtga tagtcaccca tgttgacgcc aaagctgacg cagcgttcat cagcaccgcg
    59161 aatcggcatt gttaaggcat catcagcatt gacgacacaa actttcgcat tttcgtaaat
    59221 acgcaatttt gccgcacggt attgctgcaa accaaacgga tagcggtcca tatgatcttc
    59281 agtcacgttc agaatggtcg ccgccaccgc ctgtaagctg gaagtggttt ccagctggaa
    59341 gctcgacagt tccagcacgt acagttcgca ctcagcatca agcagcatca acgctggcag
    59401 gccaatattg ccacccacac caacgttaac ccccgccgct ttcgccattt cacccactag
    59461 cgtggtgacc gtgcttttgc cgttagaacc ggtaatcgcc acaatcggtg cttgtgcttc
    59521 gcggcagaac agctcgatat cgccaacgat ttcgattccg gcatcagcgg cagcgcttaa
    59581 ggatggatgc gccagtgcaa taccgggact ggcgacaatc agatctgccg ccatcagcca
    59641 ttcatcattc agactgcccg tgtggcgttc tacggcttcg ggtaatttat ccaggccagg
    59701 cggtgtcata cgcgtatcca taacgcgcgg cgtcacaccg cgagcgagga aaaagtccac
    59761 gcaggaaagc ccagtgaggc ccaggccgat aatgacgaca tttttaccct gataatcagc
    59821 catgattaac gtaccttcag cgttgccaga ccaatcagaa ccagcatcag cgaaataatc
    59881 cagaaacgca caatgacgcg cggttccggc cagcctttca gttcatagtg gtgatgaatc
    59941 ggtgccatgc ggaaaatacg ttgtccgcgc agtttaaagg agccgacctg caggatgaca
    60001 gaaagcgttt ctaccacgaa cacgcccccc ataatcacca gcaggaattc ctgacgtagc
    60061 agtacggcga taatgcctaa cgcaccacct aacgccagcg aacctacatc gcccataaag
    60121 acctgcgccg gataggtgtt aaaccacagg aagcccagtc ctgccccgac tatcgccgtg
    60181 cagacaatga ccagttcccc ggcgtgtcgc agatacggta tgtgtaaata gctggcaaag
    60241 ttcatgttgc cggtcgccca cgccaccagc gcaaaaccac cggcgacaaa tacggtcggc
    60301 ataattgcca ggccgtcgag accatcggtc aggtttaccg cgttgccagt acccacaatg
    60361 acgaagtaag ccagcagaat gtagaacaac cccagctgcg gcatcacatc tttaaagaat
    60421 gggaccacca gctgcgttgc gggcgtgtct ttgccggcaa ggtacagggc gaaggcgata
    60481 cccagcgcaa tgaccgacat ccagaaatac ttccaacgag cgatcaaccc tttggtgtct
    60541 ttacgcacca ctttgcgata atcatcaaca aagccaataa caccgtaacc taccagcacc
    60601 accaacacgc accagacgta cggattggac gggtaagccc acagcagtac ggagatcaca
    60661 atcgccgtca ggatcataat cccgcccatg gtcggcgtac cgcgcttgct gaagtgtgat
    60721 tcaggaccgt cgttacgcac cacctgacca aaggaaagtt tttgcaaatg agcaatcata
    60781 cgcgggccca tccacaatga gatgaacagc gcggtcagca ggctgacgat ggcgcgaaac
    60841 gtcagatagg aaaagacgtt aaagccggaa taatatttga ccaaatgttc ggccagccaa
    60901 actaacatgt cccattctcc tgtaaagcgc gtactacctc ttccatggcg gcactacgtg
    60961 aacccttaac taaaatcgta attacctgtt gctcagcaat cagtgattta agacgcgtaa
    61021 ttaacgcagt tttatcagca aaatgttcgc caacgccgct ggcggtgctg atagcatggc
    61081 tttgtttacc cacgcttaac acgcggtcaa taccagcagc ttttgccgcc tcgcccacct
    61141 gtacatggca ggcttcgctt tcagcgccca gttccgccat atcgcccacc accagcacgc
    61201 ggtagcccgg catttcagcc agtacctgaa cagcggcggt cattgaaccg acattggcgt
    61261 tgtaggagtc gtcgagcagc aactggtttt ctgccagttg gatggggaac agacggcctg
    61321 gaacagcttt cagatttgcc agccccgctt tgatagcatc aagcgttgcg cccacggaca
    61381 tggagagcgc agcggctgcc agcgcattcg caatattgtg acgccccggc aacggcagca
    61441 gaacatcgac gctaccggtt ggggtttgta gggtaaattc cgtaccgtgc gaggtcacat
    61501 ggatattggt ggcggtgaaa tcgctgttgg cggcattggg tgagaaacgc cacactttgc
    61561 gtgagccaat tacgctctgc cagttcagcc agtcgttgtt gtcggcgttc ataatggcga
    61621 taccgttttc cggcaggccg ctaaagattt cacctttcgc tttcgcgaca cccgcaagcg
    61681 agccaaaacc ttccagatgc gccgctgcca ggttgttgac cagcgcagct tccgggcgag
    61741 tcagactcac agtccaggct atttcgccct gatggttcgc gccaagttca ataactgcgt
    61801 aatcgtattc cggcgttaag cgcaacagcg tcatcggtac accgatgtcg ttgttgagat
    61861 tgcctgccgt ataaagcgtg ttgccgcact ggcttaaaat cgccgccgtc atctctttaa
    61921 cggaggtttt gccggaggac cccgtcagag caaccacgcg cgccggaact tgctggcgaa
    61981 cccatgcagc cagttcacca aacgccagac gcgtatcctt gacgattaac tgcggcaggt
    62041 cgatgtccag cggacggcta accagtagtg cgcctgcgcc gccagctttc gcctggtcgg
    62101 caaaatcgtg ggcatcaaaa cgttcgcctt tcagggcaac aaacaggcag cccggcgtca
    62161 gttttcgggt atcagtggtt acagcatcaa gggtgatatc tgcaccttgc agttcaccgt
    62221 tgagaatatc ggtaagttgg ctaagggtta cgctaatcat gcaatcaccc ccagcagacg
    62281 cgccaccgtg acgcgatcgg agtagtccag acgctgattg ccaacaatct ggtaatcctc
    62341 atggcctttg cccgcgacca gtaccacatc attctcttta gcctgcataa cggcgcaagt
    62401 caccgcttca gcacggcctt ccatcacttt ggcatgtccg gcatctaaca ttcccgccag
    62461 aatatcgttg atgatggcac gcggttcttc ggtacgcggg ttatcgtccg tcaccaccgc
    62521 cacgtcagca aactcttcgg caattgcgcc catcagtgga cgcttacctt tatcgcgatc
    62581 gccaccacag ccaaagacgc accacagctt gcccgcacag tgcaggcgcg ccgcctgtaa
    62641 ggctttttcc agtgcatccg gcgtatgcgc gtaatccacc accaccgtcg gtttgcctgg
    62701 cgcagtgaac acttccatac gtccgcaaac cggttgcaga cgcgcggcgg tttttaacag
    62761 atcagccagc gggtagccaa gtgccagcag tgtcgccagc gcgagcagca ggttgctgac
    62821 gttaaaagcg cccatcagat ggctttcaat ttcgccatcg ccccaacttg aactaaagcg
    62881 aatggtcgcg ccgctgtcgt gatagttcac ttcggtcgct ttcaaccagc gtccgtgaca
    62941 gttcggattg atatgatctt ccattgatac cgcaaccgcg tccggcagtt ttgccagcca
    63001 gcggcggccc acttcatcgt cggcgttaac aatcgcctga ccacaatgat gttcagaata
    63061 aagcagccat ttcgcggctt cgtagtgttc catatcacca tgataatcaa ggtgatcgcg
    63121 gcttaagttg gtaaagaccg acgccgcaaa tttcaatgcc gccacacggt gctgtaccag
    63181 cccgtgggag gaaacttcca ttgcgcaaaa cgtcgcgccc tgatccacca gccccgccag
    63241 ctcatgctga acatcgactg ccgaaccggt ggtattttct gtcgggatca ctttccccag
    63301 cagaccgtta ccaacggtgc ccattaccgc gctggtttcg ccaagcagtt ggctccactg
    63361 cgccagcagc tgggtagtcg tggttttgcc gttggtgccc gttacgccta cgagacgtaa
    63421 attgtcagag ggttcatggt aaaagcggcc cgccagtgca gataaacgct cgttgagctg
    63481 gctgagatag atgaccggta cgccgtgcat ttcacggatt tcaccgtcgg tcgcctcacc
    63541 tttcgcctct gcaataatgg cagccacacc ttgcgctatc gcctgcggga tatatcgacg
    63601 cccgtccgcc tgatgaccta ctacagctac aaagagatcg cccgccgcag ccacacggct
    63661 gtcgagtgtc atctctcgca gtgctcgcga aggtgcgtct ggcacccacg gagcaagaag
    63721 gtcgcgcaaa ttacgatctg ccacctgtcc cctcgccttg attaatcaca aattcatttt
    63781 tatcgcccgt tgtcagcgca tccggctcga tgttcatggt acgcaatacg ccgcccatga
    63841 tggcaccaaa gaccggcgct gaaacggcgc cgccgtagta tttacccgcc tgcggatcgt
    63901 tgataacaac aaccagcgcg aagcgcggct gactcgcagg cgcaacgcct gcggtataag
    63961 caatatattt attgatgtag cgaccgtccg gcccgacctt tttcgcggta ccggttttaa
    64021 tggcgatacg atagccttta atcgccgcct tcacgccgcc gccgcctggt agcgccacgc
    64081 tttccatcat atgcaccaca gtgcggacaa tggattccgg gaagacacgt tcaccgggaa
    64141 ccggggggtc aactttggta atcgacagtg ggcgataaat gccgtagctg ccgatagttg
    64201 cgtagactcg cgctaactgt aacggtgtta ccattagccc gtagccgaaa gagaaggtgg
    64261 ccctctctat gtcagaccac cgttgttttt gaggatataa gccactgcgt tctccgacca
    64321 accccaaatt ggtcgctttt cccagtccaa aacgtgagta agtatctact aacgctgagg
    64381 acggcatcgc taacgccagc ttggaaacac cgacgttact cgacttctgt aataccccgg
    64441 tcagggttaa ttcgctgtag cgtgccacgt ctttgatttc gtggccgtta attcgataag
    64501 gaatggtatt gagtaccgag ttttcccgca ccacgccacg ttgcaacgcg gtcattacca
    64561 ccatcggttt aaccgttgag cccggttcaa acacgtcggt gatggtacgg ttacgcatcg
    64621 cctctttcgg cgtgccgctc agattgttag ggttgtatga cgggctgtta gccatcgcca
    64681 gcacttcacc ggtgttgaca tccaccagca cggcgctacc agattcagcc ttgttaaagg
    64741 ccaccgcgtt gttcagttcg cgataaacca gcgcctgcag gcgttcatca atactcagcg
    64801 ccaggttgtg cgctgcctgg ctgtcagtag aagaaatatc ttcaattacg cgaccatagc
    64861 ggtctttacg cacaatgcgc tcacccggct gcccggtaag ccatttatcg aaactcttct
    64921 caacgccctc aatcccttga ctatcgacgt tagtaaagcc gatgaggtga gcagtcactt
    64981 cgccggacgg atagtaacgg cgagactctt cacgcagatg aatccccggc agtttcagtt
    65041 ttttgatgta gtccgccatg tcagggttca cctgacgcgc cagataaata aagcgccctt
    65101 tcgggttggc gttaatgcgg gctgaaagct gatccagcgg aatattgagc gcgttagcca
    65161 gcgccttcca gcggtcaccg acgctgatac cgccagcgtc atgcacttct ttcgggtcag
    65221 cccaaatcgc ttttaccggc acgctcaccg ctaacgggcg accagaacgg tcagtaatca
    65281 tgccgcggga ggtggaaact tgctgaacgc gaagagaacg catgtcgccc tctttcacca
    65341 gcatatccgg ggagataact tgtaaccacg ctacgcgtcc gagcagaaaa gccagcgcca
    65401 ggagaataca gccgcataac aacgcaaaac gccaactgat aaagttggca tgttcttcct
    65461 gacgttttgg tttctgcgtt ttcgccgctg ctttcatgcg tcgcgtttat ccttattttt
    65521 gcactacgat attttcttgt gacggatcaa catgctgcat ttgcagcttt tccgtggcga
    65581 tcctttccac ccggctatgg tcgccgagcg cattctcttc aaggatcagg ttgcgccatt
    65641 caatatctaa agcatctcgc tccagcacca gttgttcgcg ctgagcggtc agtaaacggg
    65701 tatggtgcgc cgtggttacc acagtcaccg ccgtcaaaat aatgcaaatg aacaggcaga
    65761 gtggcagctt cccaaatcgc aaaagatcgt caccgataac accaggcaat gcatggcgct
    65821 cgtggcttcc catcgatcct ttaactttgc ttagagcttc tgtcactctg ctgatcatgc
    65881 attcgtcctc tctgcaatac gcagaactga actacgggca cgagggttct cagccacctc
    65941 ttcttcgccc ggcattaact tgcctagtgc tcgcagctga cggccaccca gttttttgag
    66001 ctgctcttca gtcatcggta accctgccgg aacttgcgga ccgcggctgt tttcacgcat
    66061 aaaacgtttc acaatacggt cttccagcga gtggaagctg atgatcgaaa gccgcccacc
    66121 cggggccagc acgttgagcg agctttttag cgcctgctct atctcctcca gttcactgtt
    66181 tacccaaatg cgcaccgcct ggaaggtacg ggtcgcggga tgtttaaact tatctttcac
    66241 cggcgttgca gcagccacga cttccgccag ttctttggtg cgggtcatcg gctgttcgcg
    66301 gttacgctcg acaatggcgc gggcaatgcg tttggcaaaa cgctcttcac cataggtttt
    66361 caatacccag gcgatatcgg cttcttctgc ggtttgtagc cattcagcgg ctgactgccc
    66421 acgggttggg tccatacgca tgtccagcgg accatcgcgc ataaaggaaa agccgcgctc
    66481 agcatcatca agttgcggtg aagagacgcc aagatcgagg agaatgccgt cgatcttgcc
    66541 gataagatcg cgctcggcaa cgtattcgcc cagcgcggag aaaggtccgt ggatgatgga
    66601 gaagcgcgga tcatcaatag tcttcgcaac ggcgatagcc tgcgggtcgc gatcgatcgc
    66661 cagcaaacgc ccctcttcgc caagctgcga gaggatcaga cgtgagtgac caccgcgacc
    66721 aaaagtccca tcaatgtaga tgccatcagg acggatattg aggccattaa cggcttcatc
    66781 cagcagcacc gtagtatgtt tatagttttc catcatttta tagagacaag tcctgcagtc
    66841 gctccgataa gtctccggta gccaactgct ctgcgtcgat atcttccttg acctgttgat
    66901 gccaggttgt ttcatcccac agctcaaact tgttgaactg tccaaccagc atcacttctt
    66961 ttgtcagccc ggcatgttgc cgcagtactg gcgcgattaa caatcgacct gcgccatcca
    67021 tctgacattc gctggcatga cctaacagta ggcgctgcac acggcgctca accgggttca
    67081 tgctcgacag acgcgataat ttttgctcga taatttccca ttcaggcagg gggtaaagca
    67141 gcaggcacgg gtgatgaatg tcaatggtgc aaaccatttg accggcagcg ttctcaagca
    67201 gctgttcccg gtaacgggta ggcactgata agcgcccttt gctgtcgaga ttgactaacg
    67261 ttgctccccg gaacatgcca gcctcacccc ttattcccac tttgccccac aatttcccac
    67321 tgaaaggagt ttacggagcg gaggaaaagc ttgtcaagcc agtcacaagg cataccgagg
    67381 taaaaaaccc gatgtttacg gctaatatca gcgttgttta ttttacgtcc aacgtgcgca
    67441 gcaaattaac gttatgaata gctgtaagaa aaaatccctc atcgtcatca ccgttttaaa
    67501 atgactcact attttccaca gcaaatataa agtgtcagtt tgcgacgcga gcggcatttt
    67561 aggacatatc ttccccggtt aacagtccct gttgcgtctg tgtggcgctt gatgccagcg
    67621 ggtttgcgca aaagcaaaga aaagtgcttg ttaaatcggc aattcatctc tgcatgtaac
    67681 aaaataatgc aaaaacgcgc tgtcgatatc acttaattat tgtgaatttc agctggtgag
    67741 ttaattaacg ggcgggttta aaagaactta tcaataatag tcaggaatag tcttatttac
    67801 tttaaggata ttgatgtcca ttcccgtact ctacgcgcca gagggaaatt cacctggcgc
    67861 gtatttttgt tcgcggctta gctacggctg agcacaccgc ggcgatagag attacgttta
    67921 atgcgcgtta aaccaggttt tggcttacgc ggttcgtcca ggcttgccag gacaatctcc
    67981 agcacacgct ctgcgacatc gcggtgacgt tgagccactg ccagcaccgg gcactgtaag
    68041 aagtcgagca gttcgttatc gccaaaggtg gcaattgcca ggtcagaagg cagtttacca
    68101 tcgcgacgta gcgtgacatc catcacccct tgcaacaacg caaacgacgt tgtgaacagc
    68161 gcctgcggca tcggatgcgt ttccagccat ttttcgaata actgggcagc cgcctcccgc
    68221 tcatagctgt tggcatacag gaaatgcact tcgcgcggat catctttcca ggcagtacgg
    68281 aaaccttgtt cacgcaggaa gctgacagaa agctccggca gcgcgccaag ataaagcacc
    68341 gtctcggcgg gaaacttacg taactcttcc gccagcattt cggcatcatc ctgatcggca
    68401 ccaaccacgc tggtgaaatg ttcacgatcg agggcgcggt ccagcgcgac aatcgggaac
    68461 gggtcgttag cccagcgttg ataaaaagga tgctcaggtg gcaacgacgt cgaaacaata
    68521 atggcatcaa cctgacgctg taaaaggtgc tcaatgcagc gcatttcgtt gtctggctga
    68581 tcttctgagc aggcaatcag cagttgataa ccccgttgcc gcgcctggcg ctcaagatag
    68641 ttagcgatgc gggtatagct ggtgttctcc agatcgggga tcacaagacc aatagaacgt
    68701 gtgcgtccag cacgaagccc agctgccacg gcgttcgggt ggtaattgtg ctcacgcacc
    68761 acagccatga ctttttcaac ggttttgtcg ctcacacggt attgcttcgc tttgccgtta
    68821 ataacatagc ttgcagtggt ccgcgacact cccgccagcc gagcgatttc atccagtttc
    68881 acaattgccc cttgcgtaaa atgtaaaaac catagccatt gtactggtat gggttaaatt
    68941 cattaacatc taagcgcaga aaattaactg cggcaactgc ttttattcca ggttcccact
    69001 gatttcgcaa aaaaagccca acgtgacatg ttgggctgca aattgcgcac tgagatcatt
    69061 ctcaacgcat tattttatcg ccgcgcgaaa gtccgaccac accagagcga gcaacttcca
    69121 caattttcgc cacatcgcga atcgatgcta aaaatgcatc aagcttatcg ctggtgcctg
    69181 ctaattgaac ggtataaagc gagggggtga catcgataat ttgcccacgg aatatttccg
    69241 tattacgttt cacttcgtca cgcccgtaac cgctggcctg aattttcacc agcatgattt
    69301 cccgctcaac atgcgcgccc tgccccaact cactcacgcg caagacatcg accagcttgt
    69361 gtaattgctt ttcgatctgc tcaagcactt tttcatcgcc cacggtctgg atggtcatac
    69421 gcgataatgt cggatcgtcg gttggcgcaa cggtcaggct ttcaatgttg tagccacgct
    69481 gggaaaaaag gccaatcacg cgggataacg cgcctgattc attttcgagt aagactgata
    69541 atatccggcg cataatcagg ttctctccgt tttgcttaac cacatttcat ccattccgcc
    69601 cccgcgaatc tgcatcgggt agacgtgctc actgccatcg acggtaacat caacaaacac
    69661 caggcgatta ttgcacacct gttccagcgc ctcgctaagt ttgctttcca gctcatgcgg
    69721 atgagagatc tggatcccga cgtgaccata ggcttccgcc agacggacga aatcgggtag
    69781 tgattgcata taagattgtg aatgacggcc ggaatagatc atgtcctgcc actgtttcac
    69841 catccccaga tagcggttat tgagattcac caccagtacc ggcaactcgt attgcaacgc
    69901 ggtagacagt tcctggatgt tcatctgaat actgccgtcg ccagtgacgc aaaccacggt
    69961 ttcttctggc aacgccattt tgacgcccag tgccgcaggt aaaccaaaac ccatcgtgcc
    70021 gaggccaccg gaattgatcc agcgacgcgg tttgtcgaat ggataataaa gtgcagcaaa
    70081 catctggtgc tgcccgacat cggacgtcac gtaagcgtct cccttcgtca accgccaaag
    70141 agtctcgatc accgcctgcg gtttaatctt ttcactgtga gtgtcatatt tcaggcactg
    70201 acgagcgcgc cactgttcaa tttgctgcca ccagtcgcgg atctcatcca gtggttgatg
    70261 ggcggattct tgcgacaaga gttcaagcat ttgttcgagg acctggcgag catcccccac
    70321 aatcgggata tccgcagtca cggttttaga aatggaagta ggatcaatat cgatatgcag
    70381 aacagtggca tttgggcagt actttgccag attgttcgtc gttcggtcat caaatcgtac
    70441 cccgacggcg aaaatcacat ccgcgttatg catcgtcata ttggcttcgt aggtaccgtg
    70501 cattcccagc atgcccagcg cctgacgatg cgttgccgga aacgccccca gccccatcaa
    70561 tgaggaaaca acgggcagat tcaacgcctc caccgtttct ttcaactgct gatggcagcc
    70621 cgccatgatt gccccaccgc ctacgtagac aaccggtttt tttgccgcta ccagcgtttg
    70681 cagagcgcgc ttaatttgcc ctttatgtcc ggtagtagtg ggattgtaag aacgcatact
    70741 gaccgactca ggccagacat agggtaattt gttcgccgga ttaagaatat ctttcggtaa
    70801 atcaacgacc actggtcctg ggcgaccact tgccgccagc cagaaagcct ttttcagcac
    70861 ctgcggaatg tcttccgttt gcttaaccag aaaactgtgt ttaaccaccg gtcgcgaaat
    70921 ccccaccatg tcgcactcct gaaaggcatc gtaacctatc aacgaggtcg ctacctgccc
    70981 ggaaaggaca actaatggaa tggaatccat ataagcggtg gcgatgccgg taatcgcatt
    71041 ggtcgcccct ggacccgacg ttaccagcac gacgccgact tccccggtcg cgcgcgccag
    71101 accatcggcc atatgcactg ccgcctgctc atgacgaact aaaacatgat cgataccacc
    71161 cacggtatgc aatgcatcgt aaatatcaag gactgcgcct ccgggataac cgaatacttg
    71221 tttaacgccc tgatcgataa gcgatcggac gaccatctcg gctccagaca acatctccat
    71281 ggcctgcctc actgtttgac ggaaaaaatg tgtaaaacac attaaccgtc cgatcttgct
    71341 taagcaattg gcagcaagag acataaacgc agggtaataa gaataaacag gaggaaaggt
    71401 gaaaagaaac gataaaatcc tccattacga aggattaaat tgataataaa tcggctgaat
    71461 cccacaactt acagcaattt ctctattttt ttaattaatc ctaaaacaga
//
LOCUS       DAZFXT010000011        65712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.011, whole genome shotgun sequence.
ACCESSION   DAZFXT010000011 DAZFXT010000000
VERSION     DAZFXT010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.011"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..472)
                     /gene="folK"
                     /locus_tag="ACHKW0_000789"
     CDS             complement(<1..472)
                     /gene="folK"
                     /locus_tag="ACHKW0_000789"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HFO7473673.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLS"
     gene            complement(469..1866)
                     /gene="pcnB"
                     /locus_tag="ACHKW0_000790"
     CDS             complement(469..1866)
                     /gene="pcnB"
                     /locus_tag="ACHKW0_000790"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:1990817 - poly(A) RNA polymerase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HFO7473674.1"
                     /translation="MFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQHAISRKDI
                     SENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKLFRNCRLV
                     GRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGSIEEDAQR
                     RDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLRAVRFAAK
                     LGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEYHLFQPLF
                     PTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLLETAQKIA
                     QESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQGKRAWKLL
                     EHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDEEPSPRRR
                     TRRPRKRAPRREGTA"
     gene            complement(1926..2822)
                     /gene="gluQRS"
                     /locus_tag="ACHKW0_000791"
     CDS             complement(1926..2822)
                     /gene="gluQRS"
                     /locus_tag="ACHKW0_000791"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HFO7473675.1"
                     /translation="MTDTQYIGRFAPSPSGELHFGSLIAALGSYLQARARQGRWLVRI
                     EDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWLHEQGLSYYCT
                     CTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGIIHADEKLARED
                     FIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLFGWKVPDYIHL
                     PLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFSVEQILQSAVK
                     NWRLTAVPESAIVNSTFSNASC"
     gene            complement(2889..3344)
                     /gene="dksA"
                     /locus_tag="ACHKW0_000792"
     CDS             complement(2889..3344)
                     /gene="dksA"
                     /locus_tag="ACHKW0_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HFO7473676.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3522..4226)
                     /gene="sfsA"
                     /locus_tag="ACHKW0_000793"
     CDS             complement(3522..4226)
                     /gene="sfsA"
                     /locus_tag="ACHKW0_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HFO7473677.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4241..4771)
                     /gene="thpR"
                     /locus_tag="ACHKW0_000794"
     CDS             complement(4241..4771)
                     /gene="thpR"
                     /locus_tag="ACHKW0_000794"
                     /EC_number="3.1.4.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004113 - 2',3'-cyclic-nucleotide
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HFO7473678.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4845..7274
                     /gene="hrpB"
                     /locus_tag="ACHKW0_000795"
     CDS             4845..7274
                     /gene="hrpB"
                     /locus_tag="ACHKW0_000795"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HFO7473679.1"
                     /translation="MSSLPVAAVLSELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGFLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIREKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7470..10004
                     /gene="mrcB"
                     /locus_tag="ACHKW0_000796"
     CDS             7470..10004
                     /gene="mrcB"
                     /locus_tag="ACHKW0_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HFO7473680.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10224..12467
                     /gene="fhuA"
                     /locus_tag="ACHKW0_000797"
     CDS             10224..12467
                     /gene="fhuA"
                     /locus_tag="ACHKW0_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HFO7473681.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12518..13315
                     /gene="fhuC"
                     /locus_tag="ACHKW0_000798"
     CDS             12518..13315
                     /gene="fhuC"
                     /locus_tag="ACHKW0_000798"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HFO7473682.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLSLVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIVQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13315..14205
                     /gene="fhuD"
                     /locus_tag="ACHKW0_000799"
     CDS             13315..14205
                     /gene="fhuD"
                     /locus_tag="ACHKW0_000799"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HFO7473683.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14202..16184
                     /gene="fhuB"
                     /locus_tag="ACHKW0_000800"
     CDS             14202..16184
                     /gene="fhuB"
                     /locus_tag="ACHKW0_000800"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HFO7473684.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLDDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16219..17499)
                     /gene="hemL"
                     /locus_tag="ACHKW0_000801"
     CDS             complement(16219..17499)
                     /gene="hemL"
                     /locus_tag="ACHKW0_000801"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HFO7473685.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTSRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17665..17712
                     /gene="yadX"
                     /locus_tag="ACHKW0_000802"
     CDS             17665..17712
                     /gene="yadX"
                     /locus_tag="ACHKW0_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YadX"
                     /protein_id="HFO7473686.1"
                     /translation="MERASKMPSSYLYDQ"
     gene            17724..19145
                     /gene="clcA"
                     /locus_tag="ACHKW0_000803"
     CDS             17724..19145
                     /gene="clcA"
                     /locus_tag="ACHKW0_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HFO7473687.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19169..19234
                     /gene="yadW"
                     /locus_tag="ACHKW0_000804"
     CDS             19169..19234
                     /gene="yadW"
                     /locus_tag="ACHKW0_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small protein YadW"
                     /protein_id="HFO7473688.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19227..19571
                     /gene="erpA"
                     /locus_tag="ACHKW0_000805"
     CDS             19227..19571
                     /gene="erpA"
                     /locus_tag="ACHKW0_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HFO7473689.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19618..20241)
                     /gene="yadS"
                     /locus_tag="ACHKW0_000806"
     CDS             complement(19618..20241)
                     /gene="yadS"
                     /locus_tag="ACHKW0_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HFO7473690.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20279..21079)
                     /gene="btuF"
                     /locus_tag="ACHKW0_000807"
     CDS             complement(20279..21079)
                     /gene="btuF"
                     /locus_tag="ACHKW0_000807"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HFO7473691.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21072..21770)
                     /gene="mtnN"
                     /locus_tag="ACHKW0_000808"
     CDS             complement(21072..21770)
                     /gene="mtnN"
                     /locus_tag="ACHKW0_000808"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008782 - adenosylhomocysteine
                     nucleosidase activity [Evidence IEA];
                     GO_function: GO:0008930 - methylthioadenosine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0009164 - nucleoside catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HFO7473692.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21854..23371
                     /gene="dgt"
                     /locus_tag="ACHKW0_000809"
     CDS             21854..23371
                     /gene="dgt"
                     /locus_tag="ACHKW0_000809"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008832 - dGTPase activity [Evidence IEA];
                     GO_process: GO:0006203 - dGTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HFO7473693.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLYPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23501..24925
                     /gene="degP"
                     /locus_tag="ACHKW0_000810"
     CDS             23501..24925
                     /gene="degP"
                     /locus_tag="ACHKW0_000810"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HFO7473694.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSSIYLLMQ"
     gene            25080..26237
                     /gene="cdaR"
                     /locus_tag="ACHKW0_000811"
     CDS             25080..26237
                     /gene="cdaR"
                     /locus_tag="ACHKW0_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HFO7473695.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTELDLGNFDDRLLLYVALQLDEER"
     gene            complement(26326..26712)
                     /gene="yaeH"
                     /locus_tag="ACHKW0_000812"
     CDS             complement(26326..26712)
                     /gene="yaeH"
                     /locus_tag="ACHKW0_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HFO7473696.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27024..27848)
                     /gene="dapD"
                     /locus_tag="ACHKW0_000813"
     CDS             complement(27024..27848)
                     /gene="dapD"
                     /locus_tag="ACHKW0_000813"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HFO7473697.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27879..30551)
                     /gene="glnD"
                     /locus_tag="ACHKW0_000814"
     CDS             complement(27879..30551)
                     /gene="glnD"
                     /locus_tag="ACHKW0_000814"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HFO7473698.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30613..31407)
                     /gene="map"
                     /locus_tag="ACHKW0_000815"
     CDS             complement(30613..31407)
                     /gene="map"
                     /locus_tag="ACHKW0_000815"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HFO7473699.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31775..32500
                     /gene="rpsB"
                     /locus_tag="ACHKW0_000816"
     CDS             31775..32500
                     /gene="rpsB"
                     /locus_tag="ACHKW0_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HFO7473700.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32758..33609
                     /gene="tsf"
                     /locus_tag="ACHKW0_000817"
     CDS             32758..33609
                     /gene="tsf"
                     /locus_tag="ACHKW0_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HFO7473701.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33756..34481
                     /gene="pyrH"
                     /locus_tag="ACHKW0_000818"
     CDS             33756..34481
                     /gene="pyrH"
                     /locus_tag="ACHKW0_000818"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HFO7473702.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34773..35330
                     /gene="frr"
                     /locus_tag="ACHKW0_000819"
     CDS             34773..35330
                     /gene="frr"
                     /locus_tag="ACHKW0_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HFO7473703.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35422..36618
                     /gene="ispC"
                     /locus_tag="ACHKW0_000820"
     CDS             35422..36618
                     /gene="ispC"
                     /locus_tag="ACHKW0_000820"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HFO7473704.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36807..37565
                     /gene="ispU"
                     /locus_tag="ACHKW0_000821"
                     /gene_synonym="uppS"
     CDS             36807..37565
                     /gene="ispU"
                     /locus_tag="ACHKW0_000821"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002094 - polyprenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008834 -
                     di-trans,poly-cis-undecaprenyl-diphosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0043164 - Gram-negative-bacterium-type cell
                     wall biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HFO7473705.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37578..38435
                     /gene="cdsA"
                     /locus_tag="ACHKW0_000822"
     CDS             37578..38435
                     /gene="cdsA"
                     /locus_tag="ACHKW0_000822"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HFO7473706.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38447..39799
                     /gene="rseP"
                     /locus_tag="ACHKW0_000823"
     CDS             38447..39799
                     /gene="rseP"
                     /locus_tag="ACHKW0_000823"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HFO7473707.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39829..42261
                     /gene="bamA"
                     /locus_tag="ACHKW0_000824"
     CDS             39829..42261
                     /gene="bamA"
                     /locus_tag="ACHKW0_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HFO7473708.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42383..42868
                     /gene="skp"
                     /locus_tag="ACHKW0_000825"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42383..42868
                     /gene="skp"
                     /locus_tag="ACHKW0_000825"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HFO7473709.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42872..43897
                     /gene="lpxD"
                     /locus_tag="ACHKW0_000826"
     CDS             42872..43897
                     /gene="lpxD"
                     /locus_tag="ACHKW0_000826"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HFO7473710.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44002..44457
                     /gene="fabZ"
                     /locus_tag="ACHKW0_000827"
     CDS             44002..44457
                     /gene="fabZ"
                     /locus_tag="ACHKW0_000827"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HFO7473711.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44461..45249
                     /gene="lpxA"
                     /locus_tag="ACHKW0_000828"
     CDS             44461..45249
                     /gene="lpxA"
                     /locus_tag="ACHKW0_000828"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HFO7473712.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45249..46397
                     /gene="lpxB"
                     /locus_tag="ACHKW0_000829"
     CDS             45249..46397
                     /gene="lpxB"
                     /locus_tag="ACHKW0_000829"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HFO7473713.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDIFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46394..46990
                     /gene="rnhB"
                     /locus_tag="ACHKW0_000830"
     CDS             46394..46990
                     /gene="rnhB"
                     /locus_tag="ACHKW0_000830"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HFO7473714.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            47027..50509
                     /gene="dnaE"
                     /locus_tag="ACHKW0_000831"
     CDS             47027..50509
                     /gene="dnaE"
                     /locus_tag="ACHKW0_000831"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HFO7473715.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50522..51481
                     /gene="accA"
                     /locus_tag="ACHKW0_000832"
     CDS             50522..51481
                     /gene="accA"
                     /locus_tag="ACHKW0_000832"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HFO7473716.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLSVPTICTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51580..53721
                     /gene="ldcC"
                     /locus_tag="ACHKW0_000833"
     CDS             51580..53721
                     /gene="ldcC"
                     /locus_tag="ACHKW0_000833"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HFO7473717.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     VQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53778..54167
                     /gene="yaeR"
                     /locus_tag="ACHKW0_000834"
     CDS             53778..54167
                     /gene="yaeR"
                     /locus_tag="ACHKW0_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7473718.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54232..55530
                     /gene="tilS"
                     /locus_tag="ACHKW0_000835"
     CDS             54232..55530
                     /gene="tilS"
                     /locus_tag="ACHKW0_000835"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HFO7473719.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAAGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55579..55839)
                     /gene="rof"
                     /locus_tag="ACHKW0_000836"
     CDS             complement(55579..55839)
                     /gene="rof"
                     /locus_tag="ACHKW0_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HFO7473720.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55826..56026)
                     /gene="yaeP"
                     /locus_tag="ACHKW0_000837"
     CDS             complement(55826..56026)
                     /gene="yaeP"
                     /locus_tag="ACHKW0_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HFO7473721.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56192..56737
                     /gene="yaeQ"
                     /locus_tag="ACHKW0_000838"
     CDS             56192..56737
                     /gene="yaeQ"
                     /locus_tag="ACHKW0_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HFO7473722.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSVFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56734..57156
                     /gene="arfB"
                     /locus_tag="ACHKW0_000839"
     CDS             56734..57156
                     /gene="arfB"
                     /locus_tag="ACHKW0_000839"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HFO7473723.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57170..57880
                     /gene="nlpE"
                     /locus_tag="ACHKW0_000840"
                     /gene_synonym="cutF"
     CDS             57170..57880
                     /gene="nlpE"
                     /locus_tag="ACHKW0_000840"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HFO7473724.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58035..58858)
                     /gene="yaeF"
                     /locus_tag="ACHKW0_000841"
                     /pseudo
     CDS             complement(58035..58858)
                     /gene="yaeF"
                     /locus_tag="ACHKW0_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(58912..60630)
                     /gene="proS"
                     /locus_tag="ACHKW0_000842"
     CDS             complement(58912..60630)
                     /gene="proS"
                     /locus_tag="ACHKW0_000842"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HFO7473725.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60742..61449)
                     /gene="tsaA"
                     /locus_tag="ACHKW0_000843"
     CDS             complement(60742..61449)
                     /gene="tsaA"
                     /locus_tag="ACHKW0_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HFO7473726.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61446..61850)
                     /gene="rcsF"
                     /locus_tag="ACHKW0_000844"
     CDS             complement(61446..61850)
                     /gene="rcsF"
                     /locus_tag="ACHKW0_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HFO7473727.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(61968..62783)
                     /gene="metQ"
                     /locus_tag="ACHKW0_000845"
     CDS             complement(61968..62783)
                     /gene="metQ"
                     /locus_tag="ACHKW0_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HFO7473728.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62823..63476)
                     /gene="metI"
                     /locus_tag="ACHKW0_000846"
     CDS             complement(62823..63476)
                     /gene="metI"
                     /locus_tag="ACHKW0_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HFO7473729.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63469..64500)
                     /gene="metN"
                     /locus_tag="ACHKW0_000847"
     CDS             complement(63469..64500)
                     /gene="metN"
                     /locus_tag="ACHKW0_000847"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HFO7473730.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64688..65260
                     /gene="gmhB"
                     /locus_tag="ACHKW0_000848"
     CDS             64688..65260
                     /gene="gmhB"
                     /locus_tag="ACHKW0_000848"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HFO7473731.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAAAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPA"
     gene            65624..>65712
                     /locus_tag="ACHKW0_000849"
     rRNA            65624..>65712
                     /locus_tag="ACHKW0_000849"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15529 a        16360 c        18108 g        15715 t
ORIGIN      
        1 tgcttaattt gtcaaatgct cttgtatgta agacttcacg caacgtctcc ccatcaggaa
       61 acgccagctc cggtgcaatc tcaaacagcg gccacagcat aaatccacga ttcttcatat
      121 cgtagtgcgg aacggtcagg cgttcggtat ttatcacttc attaccaaac agcatgatat
      181 cgagatccag cgtgcgtggt ccccagcgtt cagctttacg aacacgaccc tgttgcagtt
      241 ctatgcgctg tgtatggttg agtaactctt ccggggcgag cgaagtttca agcgccacgg
      301 cggcgttgag gtaatcgggt tgatcttgcg gccccagcgg tggggtgcgg taaaacgaag
      361 aaacggcaag aatgtggctt tcagggatat cgcctaatgc tttcagggca gcattgacct
      421 gctccagcgg agaggccaga ttgctgccta tggcaatata cgccactgtc atgcggtacc
      481 ctcacgacgc ggtgcgcgtt tgcgtggacg acgagtacga cgacgcggtg acggttcttc
      541 atccaactcg ttaagcatcc ctttttgatc tggtggcgcg gaaacctgga actcacccca
      601 ccatttcacc agacgctgca gttcagcgtt acgttcaact tcagctcgca aggccaacag
      661 gtcataagcc gcacggaact taggatgctc cagcagtttc catgcacgtt taccctgacg
      721 tcgggacata cgcaactgca actgccagat atcgcgggtt aacgtcgtca gacgtttcgg
      781 gattgccagt gaacggcagg cttcgtccag cacgtcgttc atcgccagcg cgaaagcgtc
      841 gtgataggtc aggccgcttt cctgggcgat cttctgtgcc gtctccagca gtgggtacca
      901 gaacatggcg gcaaacagga acgccgggtt cacgcgcata tcgttatgga tacgcgtatc
      961 ggtattcttc agcacctgtt caatgatccg ctccatcggg ctgtcgccat tttccgtgaa
     1021 gtagcgggta atggtcggga acagcggctg gaacagatga tattcacaca gcagcttata
     1081 ggtttcgtaa ccgtagcccg cttgtagcag tttaagcgat tcttcaaaca ggcgtgccgg
     1141 tgggatatcg ttcagcaggg tagcgaggcg agggatcggt tctgcggttt ccgggctgat
     1201 gcgcataccc aatttggcgg caaaacgtac cgcgcgcagc atacgtaccg gatcttcacg
     1261 gtagcgcgtt tccgggttac caatcagacg gataacgccg tccttcagat ccttcatacc
     1321 gccaacgtaa tcacggacgg taaaatccgc tacgctgtaa tacaggctgt tgatagtgaa
     1381 atcgcggcgc tgggcgtctt cttcgatgga gccgaaaatg ttgtcgcgca gcaacatgcc
     1441 gttttgcccg cgttgggagg tcgtgcggtc gctgacgtta ccttcgtggt gtccacggaa
     1501 ggtcgcaact tcgataatct ccgggccaaa catcacatga gccagacgga aacggcgacc
     1561 caccaggcgg cagttacgga acagtttgcg cacctgctca ggcgtggcgt tagtggttac
     1621 gtcaaagtct ttcggctttt tgccaagtaa caggtcgcgc acgccgccgc caaccagcca
     1681 ggcttcgtat cccgctttgt tgagcctgta cattaccttc agggcatttt cactgatatc
     1741 tttgcgggaa atagcatgct gctcacgcgg gatcaccgtc acctgtggac gggcgactgc
     1801 ctgttcagcc tcgctttcct cgcggcttag caccttgcgg caaaaattag cgactcgggt
     1861 aaaaatagta cacctcggta gtgtcaaaca tcattcagga caaaaaaata gcggctaatc
     1921 atagctcagc atgacgcatt tgagaatgtt gaatttacaa ttgccgactc gggcacggcg
     1981 gtaagccgcc agtttttgac ggctgactga aggatttgct cgacgctgaa atcctgccag
     2041 tgtgcttctg cctgctgccc cagaaattga agtgccgcga ttagtaccgg gcgtggatcg
     2101 cctttcggca acgcaggcgc atgattctgc ttggaaagtt tagcgccttg tggattaagc
     2161 gccagcggca gatgaatgta atctggcact ttccagccaa aaagctggta cagcgagatt
     2221 tgccttactg ttggttcaat cagatcagcc ccacgcacta tttctgtaac gccctggaaa
     2281 tgatcatcaa ccacaacagc caggttgtag gcgaacaacc catcacggcg atgaatgata
     2341 aaatcttccc gtgccagttt ttcgtcggcg tgaataatgc cgcgcagctg gtcagtaaat
     2401 tgcgtgaccg gatgctgctg gcggatacgc actgcggcgt tgtctggtcc atgatgcaac
     2461 acccggcaat gaccgtcgta aataccgcca atgctttgaa tacgcgcacg cgtacaggtg
     2521 cagtaataac ttagtccttg ttcatgtaac caggcgagtg cttcacgata ggcgtcgtga
     2581 cgttgcgatt gccagagaac atcgccgtcc cagtgcagac cgtaatgttc cagctggcgc
     2641 aggatagttt ctgcggcacc gggaacttca cgaggcgggt cgatatcttc tatgcgtacc
     2701 agccagcgac cttgccgggc gcgagcctgc aaatagctgc cgagcgcggc gatcagagag
     2761 ccaaaatgaa gctcgccgga aggagagggg gcgaagcggc caatatactg tgtgtctgtc
     2821 atctctttga acaaaaaata aggcgggagc atttcccgcc tgtggtaaac gtgatggaac
     2881 ggctgtaatt agccagccat ctgtttttcg cgaatttcag ccagcgtttt gcagtcgatg
     2941 cacagatcgg ctgtcgggcg cgcttccaga cggcgaatac caatttcaac accgcaggat
     3001 tcgcagtagc cgaaatcttc gtcttccact tttttcagcg tcttctcgat ctttttgatc
     3061 agcttacgct cgcgatcgcg gttacgcagt tcgaggctga actcttcttc ctgggctgca
     3121 cggtctaccg ggtccgggaa gttggctgct tcatcctgca tatgtgtaac ggtgcgatcg
     3181 acttcatccc tgagttgatt acgccatgct tccagaatac gacggaagtg cgccagctgg
     3241 gcttcattca tatactcttc gcccggcttc tcctgatatg gttccacccc agcgatggcg
     3301 agaatactca gggacgatgt tttacggttt tgcccttctt gcatgttgct tctccttaac
     3361 acgcactatc gatccccatg ttcgggggaa aaatgaggcc gctataaata gcagatgctt
     3421 ttccggatag caattatcta aacgtaacac ttgacaactg tgtgaggaaa agcgtatttg
     3481 cgcacgcgac cagaatgtaa attaaccagt tacttacttt actacaatgt aaccggcagt
     3541 gattttttaa gagccatgcc ttcagcagaa atttccgctt tgtaagccag aatttctacc
     3601 cccctctgtt gagcttctga caatagttgc gcgtatttct catcgatgtg gcgcgcgggt
     3661 gaaaaccgtg taatggctga atgcagcacg gcgaaaaaga taaccgcacg ctggccttca
     3721 gccgctacgc tcatcaactc ccgaaggtgt ttctgacctc gttcagtgac cgcatcggga
     3781 aaatatccct gttcgttctc cgctaacgta accgatttca cttcaatata gcagtctgga
     3841 cgcgaatccg cctgcaacat aaagtcaata cggctgcgtt ctgcgccgta ttttacttcg
     3901 cttttcagcg agctatagcc tgacagttct gaaattgatt cattaaggat agcctctttc
     3961 gtcaacctgt tagcccaaag cgtattgacg caaataaatg cgccgctctg gctttgagtt
     4021 agttcccagg tgtgtgggta tttccgtttg gtgttgtctg aagtcgaata ccagacggta
     4081 tcgccaggcg ttgcacaacc ggtcatcgca cccgtattcg ggcagtgtag cgttaattcg
     4141 cgaccatcgg gtgtgatcac atcggctaaa aaacgtttgt aacgctgaat tagcgtcgcg
     4201 cgctgtagag ggggagaaaa ttccattgcg acaatccttg ttattgcgtt agcgcccagc
     4261 gttttagcgg cgtgtagcgt gtgcgtccac gggcaaacga ggaggcgtaa agggtgaact
     4321 ccgtcaccgc ataagaccag ttaaaacctg gcggcgggat tgtcaccgcc tcgctggcgt
     4381 cgcgcaataa ggtaatatgt ggatgaaacg gacgattgct ttgaaaacaa ccgctgcggg
     4441 cagcctgtga acggagcata ttcgccagct ggattaagcc gcgtggaggc tgacgcatcc
     4501 ctaaccacac cacacgcgaa cgcagccatt gtccggcgtc atcaagcgtg agtgtgaaac
     4561 caggttgacg aatccgtccg gctaaaagag aaagcgcctt ctctttctcg gcgctcactt
     4621 cgcctaaaaa tgccagagtc agatgcaaat tatcggcggc gaccggacgt cccgcctcag
     4681 gtgggaagtg tgtggcgcgc caatggataa tctgttcgcg gatttctgca ggtaagtcga
     4741 tagcaaagaa cagacgttgc ggttcagaca tgtgaggcac tcggttatga attaccgcga
     4801 tgctacaatg tggcgcgaag aatgttaacc ctctggagcg ttttgtgtcg tcgttgcccg
     4861 ttgctgccgt cttatctgaa ttacttaccg ctctcgattg tgcgccgcag gtattattaa
     4921 gtgcgccgac cggggccggg aaatcaacct ggctgccgct gcaactgctg gcgcatcccg
     4981 gcattaacgg gaaaattatc ctgctggagc cgcgtcgtct ggcggcgcgt aacgtcgcgc
     5041 aacgactggc ggagctgctt aacgaaaagc caggcgatac cgttggctac cggatgcgtg
     5101 cgcaaaactg cgtcgggccg aatacccgcc tggaagtggt taccgaaggc gtgctgacgc
     5161 gcatgatcca gcgtgacccg gaactgagcg gtgttggatt ggtgatcctc gatgaatttc
     5221 atgagcgcag cttgcaggcg gatttggcgt tggcgctgtt actcgatgtg caacaaggtc
     5281 tgcgtgatga ccttaaactg ctgattatgt cggctacgct ggataacgac cgcttgcagc
     5341 aaatgctgcc agaagcgccc gtcgtcatct cagaagggcg ctcgtttccg gttgaacgcc
     5401 attatttacc gctgcccgcg catcagcgtt ttgacgaagc cgttgcggta gccaccgccg
     5461 aaatgctgcg tcaggaaagc ggatcattac tgttattttt acctggcgtc ggagaaattc
     5521 agcgtgtgca ggagcaactg gcttcgcgca tcggcagtga tgtgttgctc tgcccgctgt
     5581 atggcgcgtt gtcgctgaac gatcagcgaa aagcgatcct cccggcaccg caagggatgc
     5641 gcaaagtggt gctggcgacc aatattgctg aaaccagttt aaccattgaa ggtattcgtc
     5701 tggtggtgga ttgtgcccag gagcgtgtgg cgcgttttga tccgcgcacg gggcttacgc
     5761 gactgattac tcaacgcgtt agccaggcat ccatgacgca gcgtgccggg cgcgccgggc
     5821 gtctggagcc gggtatcagc ctgcatttaa tcgccaaaga acaagcagaa cgcgccgcgg
     5881 cgcaaagtga accggagatc ttacaaagcg atctttccgg ttttctgatg gaattactgc
     5941 aatggggatg cagcgatccg gcgcagatga gctggctgga tcaaccgcca acggtgaatc
     6001 tactggccgc gaaacgcctg ttacaaatgc tgggggcgct ggagggtgaa cggcttagtg
     6061 cgcaggggca aaaaatggca gcgctgggta acgatccgcg tttagcggca atgctggtta
     6121 gcgcgaagaa cgacgacgaa gctgctaccg cggcaaaaat tgccgccatt ctcgaagagc
     6181 cgccacggat gggcaatagt gacctgggcg tggcgttttc gcgcaatcaa ccagcctggc
     6241 agcaacgtag tcagcaactg ttaaaacgct taaacgtacg tggcggtgag gcagacagtt
     6301 cgcttatcgc gccgctactt gccggggcgt ttgccgatcg cattgctcgt cgccgtgggc
     6361 aagatggacg ctatcaactg gcaaacggca tgggagcgat gctcgatgcc aacgacgcgc
     6421 taagccgcca cgaatggttg atcgcaccgt tattattgca gggcagcgcc tcgccggatg
     6481 cgcggatttt actggcgtta ccggtcgata ttgatgaatt agtacaacgc tgtccgcagc
     6541 tggtgcagca atctgacacc gtggaatggg atgacgcgca aggtacgctg aaagcctggc
     6601 gtcggctgca aatcggtcag ttgacggtga aagtgcagcc actggcgaaa ccgtcagaag
     6661 acgagttgca tcaggcgatg cttaacggca ttcgtgaaaa aggtttaagc gtgctcaact
     6721 ggacggcgga agcggaacag ctacgcttgc gtttgttatg cgccgcaaag tggttgccgg
     6781 aatatgactg gccagcggtt gatgatgaaa gtttattggc gacgctggaa acgtggctgc
     6841 tgccacatat gactggcgta cattcactac gcggcctgaa atcactcgac atttatcagg
     6901 cactacgcgg attacttgat tggggaatgc agcaacgtct ggatagtgaa ttgcctgcgc
     6961 attacactgt gccgacggga agccggatcg ccattcgtta tcatgaagat aacccgcccg
     7021 cgctggcggt gagaatgcaa gagatgtttg gcgaggccac caatccgacg atcgcccagg
     7081 ggcgcgtgcc gctggtgctg gagttgcttt cacctgccca aaggccatta caaatcacac
     7141 gagatttgag cgccttctgg aaaggagcgt accgtgaggt gcaaaaagag atgaaagggc
     7201 gttatcccaa acatgtctgg ccggacgacc cggcaaatac tgcaccgacg cgacggacga
     7261 aaaagtattc gtaaggttgg ttttctccct ctccctgtgg gagagggccg gggtgagggc
     7321 atcagcgcgc acgttcaccc tcaccctaac cctctccctc aagggagagg ggaccgatcg
     7381 agcacaaatt ttgagagata tcttcttctg tcttgtaaca gaagaacaga aaatcgggct
     7441 tttgcgcctg aatattgcgg agaaaaagca tggccgggaa tgaccgcgag ccaattggac
     7501 gcaaagggaa accgacgcgt ccggtcaaac aaaaggtaag ccgtcgtcgt tacgaagatg
     7561 acgatgatta cgacgattat gatgactatg aggatgaaga accgatgccg cgcaaaggta
     7621 agggcaaagg caaagggcgt aagcctcgtg gcaaacgcgg ctggctatgg ctactgctaa
     7681 aactggctat cgtttttgcc gtgctgatcg ccatttacgg cgtttatctc gatcaaaaaa
     7741 ttcgtagccg tattgatggc aaggtctggc aactgcctgc ggcagtttat ggccgaatgg
     7801 tcaatcttga gccagacatg accatcagca agaacgagat ggtgaagctg ctggaggcga
     7861 cccagtatcg tcaggtgtcg aaaatgaccc gtcctggcga atttaccgtg caggccaaca
     7921 gcattgagat gattcgccgt ccgtttgatt tcccggacag taaagaagga caggtgcgcg
     7981 cgcgtctgac ctttgatggc gatcatctgg cgacgatcgt caatatggag aacaaccgtc
     8041 agttcggttt cttccgtctt gatccgcgtc tgatcaccat gatctcttcg ccaaacggtg
     8101 agcagcgtct gtttgtgccg cgcagtggtt tcccggattt gctggtggat actttgctgg
     8161 cgacagaaga ccgtcatttt tacgagcatg atggaatcag tctctactca atcggacgtg
     8221 cggtgctggc aaacctgacc gccggacgca cggtacaggg tgcgagtacg ctgacgcaac
     8281 agctggtgaa aaacctgttc ctctccagcg agcgttctta ctggcgtaaa gcgaacgaag
     8341 cttacatggc gctgatcatg gacgcgcgtt acagcaaaga ccgtattctt gagctgtata
     8401 tgaacgaggt gtatctcggt cagagcggcg acaacgaaat ccgcggcttc ccgctggcaa
     8461 gcttgtatta ctttggtcgc ccggtagaag agctaagcct cgaccagcag gcgctgttag
     8521 tcggtatggt gaaaggggcg tccatctaca acccgtggcg taacccaaaa ctggcgctgg
     8581 agcgacgtaa tctggtgctg cgtctgctgc aacagcaaca gattattgat caagaactct
     8641 atgacatgtt gagtgcacgt ccgctgggtg ttcagccacg cggtggggtg atctctccac
     8701 agccagcctt tatgcaactg gtgcgtcagg agctgcaggc gaaattaggc gataaggtaa
     8761 aagatctctc cggcgtgaag atcttcacta cctttgactc ggtggcccag gacgcggcag
     8821 aaaaagccgc cgtggaaggc attccggcac tgaagaaaca gcgtaagttg agcgatcttg
     8881 aaactgcgat tgtggtcgtc gaccgcttta gtggtgaagt tcgtgcgatg gtcggtggtt
     8941 ctgagccgca gtttgcgggc tacaaccgtg cgatgcaggc gcgtcgttcg attggttccc
     9001 ttgcaaaacc agcgacttat ctgactgcct taagccagcc gaaaatctat cgtctgaata
     9061 cgtggattgc ggatgcgcca atcgcgctgc gtcagccgaa tggccaggtc tggtcaccgc
     9121 agaatgatga ccgtcgttat agcgaaagcg gcagagtgat gctggtggat gcgttgaccc
     9181 gttcgatgaa cgtgccgacg gtaaatctgg ggatggcgct ggggctgcct gcggttacgg
     9241 agacctggat caaactgggc gtaccgaaag atcagttgca tccggttccg gcgatgctgc
     9301 tgggggcgtt gaacttaacg ccaatcgaag tggcgcaggc gttccagacc atcgccagcg
     9361 gcggtaaccg tgcaccgctt tctgcactgc gttcggtaat tgcggaagat ggcaaggtgc
     9421 tgtatcagag cttcccgcag gcggaacgcg ctgttccggc gcaggcggcg tatctgacac
     9481 tatggaccat gcagcaggtg gtacaacgcg gtacgggtcg tcagcttggg gcgaaatacc
     9541 cgaacctgca tctggcaggg aaaacaggga ctaccaacaa taacgtagat acctggtttg
     9601 caggcattga cggcagcacg gtgaccatca cctgggtagg ccgtgataac aaccagccga
     9661 ccaaactgta tggagccagc ggggcaatgt cgatttatca gcgttatctg gctaaccaga
     9721 cgccaacgcc gctgaatctt gttccgccag aagatattgc agatatgggc gtggactacg
     9781 acggcaactt tgtttgcagc ggtggcatgc gtgtcttgcc ggtctggacc agcgatccgc
     9841 aatcgctgtg ccagcagagc gagatgcagc agcagccgtc aggcaatccg tttgatcagt
     9901 cttctcagcc gcagcaacag ccgcaacagc aacctgctca gcaagagcag aaagacagcg
     9961 acggtgtagc cggttggatc aaggatatgt ttggtagtaa ttaacatcta agcgtgaaat
    10021 accggatggc gagttgccat ccggtaaaat aacatcccat ctaagatatt aaccctttct
    10081 tttcatctgg ttgtttatta acccttcagg aacgctcaga ttgcgtaccg cttgcgaacc
    10141 cgccagcgtt tcgaatatta tcttatcttt ataataatca ttctcgttta cgttatcatt
    10201 cactttacat cagagatata ccaatggcgc gttccaaaac tgctcagcca aaacactcac
    10261 tgcgtaaaat cgcagttgta gtagccacag cggtaagcgg catgtctgtt tatgcacagg
    10321 cagcggttga accgaaagaa gacactatca ccgttaccgc tgcacctgcg ccgcaagaaa
    10381 gcgcatgggg gcctgctgca actattgcgg cgcgacagtc tgctaccggc actaaaaccg
    10441 atacgccgat tcaaaaagtg ccacagtcta tttctgttgt gaccgccgaa gagatggcgc
    10501 tgcatcagcc gaagtcggta aaagaagcgc ttagctacac gccgggtgtc tctgttggta
    10561 cgcgtggcgc atccaacacc tatgaccacc tgatcattcg cggctttgcg gcagaaggcc
    10621 aaagccagaa taactatctg aatggcctga agttgcaggg caacttctat aacgatgcgg
    10681 tcattgaccc gtatatgctg gaacgcgctg aaattatgcg tggcccggtt tccgtgcttt
    10741 acggtaaaag cagtcctggc ggcctgttga atatggtcag caagcgtccg accaccgaac
    10801 cgctgaaaga agttcagttt aaagccggta ctgacagcct gttccagact ggttttgact
    10861 ttagcgattc gttggatgat gacggtgttt actcttatcg cctgaccggt cttgcgcgtt
    10921 ctgccaatgc ccagcagaaa gggtcagaag agcagcgtta tgctattgca ccggcgttca
    10981 cctggcgtcc ggatgataaa accaatttca ccttcctttc ttacttccag aacgagccgg
    11041 aaaccggtta ttacggctgg ttgccgaaag agggaaccgt tgagccgctg ccgaacggta
    11101 agcgtctgcc gacagacttt aatgaagggg cgaagaacaa cacctattct cgtaatgaga
    11161 agatggtcgg ctacagcttc gatcacgaat ttaacgacac ctttactgtg cgtcagaacc
    11221 tgcgctttgc tgaaaacaaa acctcgcaaa acagcgttta tggttacggc gtctgctccg
    11281 atccggcgaa tgcttacagc aaacagtgtg cggcattagc gccagcggat aaaggccatt
    11341 atctggcacg taaatacgtc gttgatgatg agaagctgca aaacttctcc gttgataccc
    11401 agttgcagag caagtttgcc actggcgata tcgaccacac cctgctgacc ggtgtcgact
    11461 ttatgcgtat gcgtaatgac atcaacgcct ggtttggtta cgacgactct gtgccactgc
    11521 tcaatctgta caatccggtg aataccgatt tcgacttcaa tgccaaagat ccggcaaact
    11581 ccggccctta ccgcattctg aataagcaga aacaaacggg cgtttatgtt caggatcagg
    11641 cgcagtggga taaagtgctg gtcaccctgg gcggtcgtta tgactgggca gatcaagaat
    11701 ctcttaaccg cgttgctggg acgaccgata aacgtgatga caaacagttt acctggcgtg
    11761 gtggtgttaa ctacctgttt gataatggtg taacacctta cttcagctat agcgaatcgt
    11821 ttgaaccttc ttcgcaagtt gggaaggatg gtaatatttt cgcaccgtct aaaggtaagc
    11881 agtatgaagt cggcgtgaaa tatgtaccgg aagatcgtcc gattgtagtt actggtgccg
    11941 tgtataatct cactaaaacc aacaacctga tggcggaccc tgagggttcc ttcttctcgg
    12001 ttgaaggtgg cgagatccgc gcacgtggcg tagaaatcga agcgaaagcg gcgctgtcgg
    12061 cgagtgttaa cgtagtcggt tcttatactt acaccgatgc ggaatacacc accgatacta
    12121 cctataaagg caatacgcct gcacaggtgc caaaacacat ggcttcgctg tgggctgact
    12181 ataccttctt tgacggtccg ctttcaggtc tgacgctggg caccggtggt cgttatactg
    12241 gctccagcta tggtgatccg gctaactcct ttaaagtggg aagttatacg gtcgtggatg
    12301 cgttagtgcg ttatgatctg gcgcgagtcg gcatggcggg ctccaacgtg gcgctgcatg
    12361 ttaacaacct gttcgatcgt gaatacgtcg ccagctgctt taacacttat ggctgcttct
    12421 ggggcgcaga acgtcaggtc gttgcaaccg caaccttccg tttctaattt ctcttttggg
    12481 gcacggattt ccgtgcccat ttcacaagtt ggctgttatg caggaataca cgaatcattc
    12541 cgataccact tttgcactgc gtaatatctc ctttcgtgtg cccgggcgca cgcttttgca
    12601 tccgctgtcg ttaacctttc ctgccgggaa agtgaccggt ctgattggtc acaacggttc
    12661 tggtaaatcc actctgctca aaatgcttgg ccgtcatcag cctccgtcag aaggggagat
    12721 tcttcttgat gcccagccgc tggaaagctg gagcagcaaa gcgtttgccc gcaaagtggc
    12781 ttatttgccg cagcagcttc ctccggcaga agggatgacc gtgcgtgaac tggtggcgat
    12841 tggtcgttac ccgtggcatg gcgcgctggg gcgctttggt gcggcagatc gcgaaaaggt
    12901 cgaggaagct atctcgctgg ttggcttaaa accgctggcg catcggctgg tcgatagtct
    12961 ctctggcggc gaacgtcagc gggcgtggat cgccatgctg gtggcgcagg atagccgttg
    13021 tctgttgctc gacgaaccga cctcggcgct ggatatcgcc caccaggttg atgtgctgtc
    13081 gctggtgcac cgtttaagtc aggagcgtgg cctgacggtc attgccgtgt tgcacgatat
    13141 caatatggcg gcacgctact gtgattatct ggtcgccctg cgcggcggtg aaatgattgt
    13201 tcagggaacg cctgcggaaa ttatgcgcgg cgaaaccctc gaaatgattt atggcatccc
    13261 gatgggtatt ttgccgcatc cggcgggtgc tgcacctgtg agttttgttt attgatgagc
    13321 ggcttacctc ttatttcgcg ccgtcgactg ttaacggcga tggcgctttc tccgttgtta
    13381 tggcagatga ataccgccca cgcggcggct attgatccca atcgtattgt ggcgctggag
    13441 tggttgccgg tggaattact gctggcgctc ggcatcgtgc cttacggcgt ggcggatacc
    13501 atcaactatc gcctgtgggt cagcgaacca ccattgccgg actcagtgat cgacgtcggt
    13561 ttgcgcacag aacctaacct tgaactgctg accgaaatga aaccatcgtt tatggtctgg
    13621 tcggcaggat atggcccttc accagaaatg ctggctcgta ttgcgccggg gcgtggattt
    13681 aacttcagcg acggcaaaca gccgctggcg atggcgcgta agtcactgac ggaaatggca
    13741 gatttactta acctgcaaag cgcagcagaa acgcatttag cgcactatga agactttatc
    13801 cgcagcatga aaccccgctt tgtgaagcgt ggtgaacgcc cgttattgct gacgacgctt
    13861 atcgatccgc gccatatgct ggttttcggt ccaaacagct tgtttcagga aattcttgat
    13921 gagtacggca tcccaaatgc ctggcaaggg gaaaccaact tctggggcag taccgccgtc
    13981 agcatcgatc gtctggcggc gtataaagat gttgatgtgc tctgttttga tcacgacaac
    14041 agcaaagaca tggacgcgct aatggcaacc ccgctgtggc aggccatgcc gtttgtccgc
    14101 gccggacgct ttcagcgcgt acccgcagtc tggttttatg gtgcgacgct ctcggcaatg
    14161 cactttgtgc gcgttctgga taacgccatc ggaggtaaag cgtgagtaaa cgaattgcgc
    14221 tttttccggc gttattgctg gcgctgctgg tgattgtcgc tacggcgctg acctggatga
    14281 acttttcgca ggcgctgccg cgtagccagt gggcgcaggc cgcctggtcg ccggatatta
    14341 acgtcatcga gcagatgatt tttcactaca gcttgttacc acgtctggcg atttctctac
    14401 tggtgggcgc gggcctgggg ctggtgggcg tgctgtttca gcaagtgctg cgtaacccgc
    14461 tggcggaacc gacgacgttg ggcgttgcta caggcgcgca actggggatt accgtcacca
    14521 cgctctgggc gatcccaggt gctatggcga gccagtttgc tgcgctggca ggggcttgcg
    14581 ttgttggctt aattgtcttt ggcgtcgcgt gggggaaacg gctgtcgccg gtaacgctga
    14641 ttctcgcggg gctggtagtg agcctttatt gtggggcaat caatcagtta ctggttatct
    14701 tccatcatga ccagttgcaa agcatgttcc tgtggagcac cggaacgctg acgcaaaccg
    14761 actggggcgg cgttgagcgt ttatggccgc agttgctggg cggcgtgatg ctgacgttac
    14821 tgctacttcg tccgttaacc ctgatggggc ttgatgatgg cgtggcgcgc aatctcgggc
    14881 tggccttgtc gctcgcgcgt ctggcggcgc tgtcgctggc gatagtcatc agtgcgctgc
    14941 tggtgaacgc agtggggatt atcggcttta tcgggttgtt cgcgccactg ctggcaaaaa
    15001 tgctgggggc gcggcgtttg ttgccacggt tgatgctggc gtcgctgatt ggtgcgttaa
    15061 ttctgtggct ttctgatcaa atcatcctct ggctgactcg cgtgtggatg gaagtttcca
    15121 ccggttcggt cactgcgttg atcggtgcgc cgctgctact gtggctgctg ccgcgtttac
    15181 gcagcattag cgcgccggat atgaaggtta acgatcgtgt cgcgactgaa cgccaacatg
    15241 tgctggcgtt tgccctcgcg ggcggcgtgc tgctgttgat ggctgtggtg gtggcgctgt
    15301 cgtttggtcg tgatgcgcac ggctggacgt gggcgagcgg ggcgctgctc gatgatttaa
    15361 tgccctggcg ctggccgcga attatggcgg cgctgtttgc gggcgtcatg ctggcggtag
    15421 cgggctgtat tattcagcgg ctgaccggca acccgatggc aagcccggaa gtgctgggga
    15481 ttagctccgg cgcggcgttt ggtgtggtgt taatgctgtt tctggtaccg ggtaatgcct
    15541 ttggctggct gttacccgca ggcagtctcg gggcggcggt gacgctgttg atcattatga
    15601 tcgccgccgg acgcggtgga ttttccccac accgcatgtt actggcgggg atggcgttaa
    15661 gtaccgcgtt caccatgctt ttgatgatgt tgcaggcaag tggtgacccg cgaatggcgc
    15721 aagtgctgac ctggatttcc ggttcgacct acaacgcgac cgatgcgcag gtctggcgca
    15781 ccggaattgt gatggtgatt ttgctggcga ttaccccgct gtgccgccgc tggctgacca
    15841 ttttaccgct gggtggtgat accgcccgag ccgtaggaat ggcgctgacg ccgacgcgaa
    15901 ttgcgctgct gctgttagcg gcttgcctga cggcgaccgc gacgatgact attggaccgt
    15961 tgagttttgt tggtttaatg gcaccgcata ttgcgcggat gatgggcttt cgacggacga
    16021 tgccacacat cgtaatttcg gcgctggtgg gggggttact gctggtgttc gctgactggt
    16081 gtgggcggat ggtgctgttt ccattccaga tcccggcggg gctgctgtca acctttatcg
    16141 gcgcgccata ttttatctat ttgttgagaa agcagagccg ttaattgaat gcctcgcccg
    16201 gtgggcgagg caaaatcatc acaacttcgc aaacacccga cgtgcagcat cgatggtgtt
    16261 attgatatct tccatgctgt gcgccacgga cataaagccc gcttcaaacg ctgacggtgc
    16321 caggtaaaca ccttcatcca gcatcatatg gaagaaacgc ttaaagcgtt ccacgtcaca
    16381 ggccatcaca tcctgatagc acgtcacgga ctcggcgtcg gtaaagaaaa taccgaacat
    16441 gccgccaacg tggttaacga ccagcggaat tccggcttct tctgccgctt ccagcagacc
    16501 ttctgccaga cgtgatgtca gctcatccaa cgtttcgtga acgcccggtt gcgcgacttc
    16561 attcagacag gcgaaacccg ctgccatcgc aattgggtta ccggaaagcg tacccgcctg
    16621 atagaccgga cccgtcgggg ccagcgcatc cattacatca cgacgaccac cgaatgcgcc
    16681 taccggcatt ccaccgccga tgattttgcc caggcaggtg agatccggtt ccacaccgta
    16741 ataatcctgt gcgccagcta gcgctacgcg gaagccagtc attacttcat cgatgatcag
    16801 caatgcgcca aattcgtcgc acagcgcgcg cagacctggc aggaactctg gcagcggtgg
    16861 aacgcagttc atattgcctg ccaccggctc gacgataata caggcaatct cttgcgggta
    16921 ttgctcaaac gcggcgcgta cagaagccag atcgttataa gtacaggtta aggtatgttt
    16981 ggcgaaatct gccggaacac ccggcgagtt tggctggcct aacgtgagtg cgccagaacc
    17041 ggctttcacc agcaggcagt cagcgtgacc gtggtaacaa ccttcaaatt taatgatttt
    17101 gtcgcgaccg gtaaaaccac gggccaggcg gatggcgctc atggtcgctt cagtgccgga
    17161 gttcaccatg cgcaccatat ccatggtcgg gaccagttcg gtcaccagtt gcgccatttt
    17221 cacttccatt tcggttggtg caccaaagct taaaccacgc tcggcggctt caatcacggc
    17281 attgcggatc gccggatggt tatggcccag caccatcggc ccccaggaac cgacataatc
    17341 gatataggct ttgccatcaa catcgtacag ataggcgccg tccgcttttt cgataaacag
    17401 tggagtgccg cccacgccag taaaggcgcg aacaggggag ttcacaccgc cagggatcag
    17461 ctcgcgcgct gcgctgtaaa gattttcaga cttactcatg gagggttcct gattcgtaga
    17521 aaaagtgaat ggctgctatt ctatgttatt cataacaagt taaatactcg tcaaacatca
    17581 ggctgcttgt actggtgcaa tcctgatttc ttagagtata aaagttttgt gcatttgaaa
    17641 cattacgctt tgcaaaggat tttcatggaa cgtgcgagta aaatgccgtc atcttatttg
    17701 tatgaccaat aagtgatcat tggatgaaaa ctgatactcc ctctttagaa acaccgcagg
    17761 ccgcgcgcct gcgacgtaga caactgattc gccaacttct tgagcgcgat aaaaccccgt
    17821 tagccatttt gtttatggcg gcagtcgtcg gcacgcttgt cgggctggca gcggttgctt
    17881 ttgacaaagg tgtcgcctgg ttgcagaacc aacgtatggg ggcgctggta catactgctg
    17941 ataattatcc gctactgtta accgtcgctt ttctctgttc ggcggtgctg gcgatgttcg
    18001 gctacttttt ggtgcgcaaa tacgcgcccg aagcaggggg atcggggatc ccggaaattg
    18061 aaggggcgct ggaagatcaa cgtcctgttc gctggtggcg tgtattgccg gtgaaattct
    18121 ttggcgggct ggggacactc ggcggaggca tggtgctagg gcgcgaagga ccaaccgtgc
    18181 agatcggcgg caatatcggt cgcatggtgc ttgatatttt ccgcctgaaa ggtgatgaag
    18241 ctcgccatac gctgctggca accggtgctg ctgcggggct ggctgcggcc tttaacgcgc
    18301 cgctggcggg tattttgttt attatcgaag agatgcgtcc gcagtttcgc tatacgttaa
    18361 tttcgattaa agcggtattt attggcgtca ttatgtcgac cattatgtac cggattttta
    18421 atcatgaagt tgcgttgatt gacgtcggta aactttctga tgcgccgctt aatacgctgt
    18481 ggctttatct gatcctcggt attatttttg gcattttcgg ccctattttt aataaatggg
    18541 tgctggggat gcaggatttg ctgcaccgtg tgcacggcgg caatattacc aaatgggtac
    18601 tgatgggtgg tgcgattggc ggtctgtgtg gattgctggg gtttgtggca ccagcaacgt
    18661 cgggcggcgg ttttaacctg attcctatcg ctaccgcggg gaatttcagc atgggaatgc
    18721 tggtgtttat cttcgtcgct cgggtcatta ccaccttact ctgcttctct tccggcgcgc
    18781 cgggcggtat ttttgccccg atgctggcgc tgggtactgt gctgggaacc gctttcggaa
    18841 tggttgccgt tgagctgttt ccgcaatatc acctggaggc ggggacgttt gctattgccg
    18901 gaatgggggc attactggcg gcatctattc gcgcgccgtt aacggggatc attctggttc
    18961 tggagatgac cgataactac cagctcattt tgccaatgat tattaccggt cttggcgcaa
    19021 cactattagc gcaatttacc ggcgggaaac cgctatactc ggcgattctt gcgcgcacgc
    19081 tggcaaaaca ggaagctgag caactggcgc gaagcaaggc cgcatcagcc agcgagaata
    19141 cttgaacgaa ataccagggt attagataat ggcgattatt attgggttag aatttgccca
    19201 attgccgatg tcgtttggag caaaatatga gtgatgacgt agcactgccg ctggagttta
    19261 ccgacgcagc agccaacaaa gttaaaagcc tgatcgctga cgaagataac ccgaatctga
    19321 aattacgcgt gtatatcacc ggtggcggtt gcagcggctt ccagtatggt ttcacctttg
    19381 atgatcaggt gaacgaaggc gatatgacca tcgaaaaaca gggcgttggc ctggtggttg
    19441 atccgatgag cctgcaatat ctggtcggcg gttccgttga ttataccgaa ggtctggaag
    19501 gttctcgttt catcgtgacc aacccgaacg cgaaaagcac ctgcggttgc ggttcttcct
    19561 ttagcatcta atctgttgtt catgtgccgg ataaactatc cggcacatct tctctcttca
    19621 acgcccattc tcatccagcg caaacgtcgg tagcttaaga tgccaacgaa tagccgccag
    19681 ccgaatcaat agcgtcacga ccatgcccat catactggct gtttccagtg gtacggaaaa
    19741 tgtgtaataa gccgtagcgt ggacaatacc gccgataata caggcagttg cgtagatttc
    19801 tgtacgtaaa atcatgggga tctcgcgggc cagtacatca cgaatgatcc cgccgccaac
    19861 gccagtaatg acgcccatgc aaaccgcgat taacggaccg gcttccgcat taaaggcttt
    19921 attcacgcca atgccgacaa acaccgccag accaacggcg tccagcaccg gcaacatcca
    19981 ttttggtaag cgtcttggct ggcgcaccag cacgatggtc agcatgctgg tgaccattgc
    20041 aacgaccaga tcggtgggat ctttcaccca aaataccggg ccgtgatcca gcgccatgtc
    20101 gcgaattgtc ccgccgccta ctgcggttac cacgcccagt accagaacac caaaagggtc
    20161 catacgcaat tttccggcta acaaaacgcc ggagatggca aataccgctg tgccgactat
    20221 atccagccaa tagacgagca ttgttcaatc cccactgagc acctgaaaag gtcaggcgct
    20281 aatctacctg tgaaagcgca ttacagagct gttgtgcagc gaggataata cgtgggcttg
    20341 cacgttcaaa ccagtcactc gtgagaggaa taacgggaat tttgagctgt tcaccccagt
    20401 attgtttgat tttaggaatt tggtccggtc cgcctgtaat gacaatcgcc tgtggcgagc
    20461 gtgctaacac ctgttcgcgg ctaacttgcg gccagggaac ccggctgtct ttaaagatgt
    20521 tttctccgcc acaaacttcg agtacctggt tctgaatcga ctcttttcca ctggtaaatg
    20581 gcggattaat gccgaattgc agaaaaacac gttttttagg tttatcagca tattgcgctt
    20641 tcaattgcgc gtactgatcc agcagggatt gcgcggcttg ttcggccttg tctggttgcg
    20701 gactccaggg ggccagttga cgtaacgcat tggcaatttg ttcaatgctt gtcgcatcga
    20761 cccacatcac ttttattccc agcgaagcca gctggtcaac ctgccgctcg gcattacctc
    20821 cacgccaggc aatcaccaga tcgggcttca gcgcgacaat gcgttccaga ttcatcccct
    20881 gccaggtgga aacctgctca atcttttgcg cttgtggagg atagtcggaa tagctgctga
    20941 ccccaaccgg cgtgatcccg gcagcaaagg caagttcagt gttggcggga gaaagcgtga
    21001 tgacgcgcgg cgcggcgttg agccacagtg gcgcaagaaa agacagggcg accagcgccc
    21061 tgaacagtga cttagccatg tgcaagtttc tgcaccagtg actcaaccat caggctggac
    21121 tgtttagcgg caacagccag gaactcatcg aagctaagat gagactgttg atcggccacg
    21181 tcggagatgg cgcgtacgac aacaaacggg acgttgaaat tgtggcagac atgggcgatt
    21241 gccgtcgctt ccatctctac agcaatggcc tgtgggaagt tgtggcggat tttcgccaga
    21301 ccaacagaac cgttgatgaa agcgtcgccg ctaacaatca ggccacgtac agcgttaaga
    21361 ttcagttcgg caatgcaggc ctcagcggca gcgatcagtt tatcgtcagc tttaaagcct
    21421 gccggacagc ctggtaactg accgtattca taaccaaatg ccgtgacatc cgcgtcgtga
    21481 taacgtgctt cgtccgagac aacgatatcg cccactttca acgttggtgc caggccaccg
    21541 gcagaaccgg tgttaataat cacatctggc ttgcagtgtt ccaacagcag agtggcaccc
    21601 agcgccgcag cgactttacc gatgcccgat ttcagaagcg caacctcggt tccattcagt
    21661 tggccggtat agatttcgca accgccgaga ctgatagttt gacggttttc gattttgtca
    21721 cgcagcagcg taacttcttc ttccattgca ccaatgatgc cgattttcat agatttactc
    21781 gcgataagcc cgatttgaag gcatagttta ccatgcgctt acggggaagc gtatttctca
    21841 cgcgggagag gacatggcac agattgattt ccgaaaaaaa ataaactggc atcgtcgtta
    21901 ccgttcaccg cagggcgtta aaaccgaaca tgagatcctg cggatcttcg agagcgatcg
    21961 cgggcgtatc atcaactctc cggcaattcg tcgtctgcaa caaaagaccc aggtttttcc
    22021 actggagcgc aatgccgccg tgcgcacgcg tcttacccac tcgatggaag tccagcaggt
    22081 ggggcgctac atcgccaaag aaattttaag ccgtctgaaa gagcttaaat tgctggaagc
    22141 atacggcctg gatgaactga ccggaccttt tgaaagcatt gttgagatgt catgcctgat
    22201 gcacgatatc ggcaatccgc cgtttggtca ttttggcgaa gcggcgataa atgactggtt
    22261 tcgccagcgt ttatacccgg aagatgccga aagccagcct ctgactgacg atcgctgcag
    22321 cgtggcggca ctacgtttac gggacgggga agaaccgctt aacgagttgc ggcgcaagat
    22381 tcgtcaggac ttatgtcatt ttgaggggaa tgcacaaggc attcgtctgg tgcatacatt
    22441 gatgaggatg aatctcacct gggcacaggt tggcggtatt ttaaaatata cccgtccggc
    22501 gtggtggcgt ggcgaaacgc ctgagacaca tcactattta atgaaaaagc cgggttatta
    22561 tctttctgaa gaagcctata ttgcccggtt gcgtaaagaa cttaatttgg cgctttacag
    22621 tcgttttcca ttaacgtgga ttatggaagc tgccgacgac atctcctatt gtgtggcaga
    22681 ccttgaagat gcggtagaga aaagaatatt taccgttgag cagctttatc atcatttgca
    22741 cgaggcgtgg ggtcagcatg agaaaggttc gctcttttcg ctagtggttg aaaatgcctg
    22801 ggaaaaatca cgctcaaata gtttaagccg cagtacggaa gatcagtttt ttatgtattt
    22861 acgggtaaac accctaaata aactggtacc atacgcggca caacgattta ttgataatct
    22921 gcctgcgatt ttcgccggaa cgtttaatca tgcattattg gaagatgcca gcgaatgcag
    22981 cgatcttctt aagctatata aaaatgtcgc tgtaaaacat gtgtttagcc atccggatgt
    23041 cgagcagctt gaattgcagg gctatcgggt cattagcgga ttattagaga tttatcgtcc
    23101 tttattaagc ctgtcgttat cagactttac tgaactggta gaaaaagaac gggtgaaacg
    23161 tttccctatt gaatcgcgct tattccacaa actctcgacg cgccatcggc tggcctatgt
    23221 cgaggctgtc agtaaattac cgtcagattc tcctgagttt ccgctatggg aatattatta
    23281 ccgttgccgc ctgctgcagg attatatcag cggtatgacc gacctctatg cgtgggatga
    23341 ataccgacgt ctgatggccg tagaacaata accaggcttt tgtaaagacg aacaataaat
    23401 ttttaccttt tgcagaaact ttagttcgga acttcgcgct ataaaacgaa tctgaagaac
    23461 acagcaattt tgcgttatct gttaatcgag actgaaatac atgaaaaaaa ccacattagc
    23521 actgagtgca ctggctctga gtttaggttt ggcgttatct ccgctctctg caacggcggc
    23581 tgagacttct tcagcaacga cagcccagca gatgccaagc cttgcaccga tgctcgaaaa
    23641 ggtgatgcct tcagtggtca gcattaacgt agaaggtagc acaaccgtta atacgccgcg
    23701 tatgccgcgt aatttccagc agttcttcgg tgatgattct ccgttctgcc aggaaggttc
    23761 accgttccag agctctccgt tctgccaggg gggccagggc ggtaatggcg gcggccagca
    23821 acagaaattc atggcgctgg gttccggcgt catcattgat gccgataaag gctatgtcgt
    23881 caccaacaac cacgttgttg ataacgcgac ggtgattaaa gtccagctga gcgatggtcg
    23941 taagttcgac gcgaagatgg ttggcaaaga tccgcgctct gatatcgcgc tgattcagat
    24001 ccagaacccg aaaaacctga ccgcaattaa gatggcggat tctgatgcac tgcgcgtggg
    24061 tgattacacc gtagcgattg gtaacccgtt tggcctggga gagacggtaa cttccgggat
    24121 tgtctctgcg ctggggcgta gcggcctgaa tgccgaaaac tacgaaaact tcatccagac
    24181 cgatgcagcg atcaaccgtg gtaactccgg tggtgcgctg gttaacctga acggcgaact
    24241 gatcggtatc aacaccgcga tcctcgcacc ggacggcggc aacatcggta tcggttttgc
    24301 tatcccgagc aacatggtga aaaacctgac ctcgcagatg gtggaatacg gccaggtgaa
    24361 acgcggtgag ctgggtatta tggggactga gctgaattcc gaactggcga aagcgatgaa
    24421 agttgacgcc cagcgcggtg ctttcgtaag ccaggttctg cctaattcct ccgctgcaaa
    24481 agcgggcatt aaagcgggtg atgtgatcac ctcactgaac ggtaagccaa tcagcagctt
    24541 tgccgcactg cgtgctcagg tgggtactat gccggtgggc agcaaactga ccctgggctt
    24601 gctgcgcgac ggtaagcagg tcaacgtgaa tctggaactg cagcagagca gccagaatca
    24661 ggttgattcc agctccatct tcaacggcat tgaaggtgct gagatgagca acaaaggcaa
    24721 agatcagggc gtggtagtga acaacgtgaa aacgggcact ccggctgcgc agatcggcct
    24781 gaagaaaggt gatgtgatta ttggcgcgaa ccagcaggca gtgaaaaaca tcgctgaact
    24841 gcgtaaagtt ctcgacagca aaccgtctgt gctggcactg aacattcagc gcggcgacag
    24901 ctccatctac ctgttaatgc agtaatctcc ctcaacccct tcctgaaaac gggaaggggt
    24961 tctccttaca atctgtgaac ttcaccacaa ctccatacat cttcatcatc ctttaggcat
    25021 ttgcacaatg ccgtacgtta cgtacttcct tatgctaagc cgtgcataac ggaggactta
    25081 tggctggctg gcatcttgat accaaaatgg cgcaggatat cgtggcacgt accatgcgca
    25141 tcatcgatac caatatcaac gtaatggatg cccgtgggcg aattatcggc agcggcgatc
    25201 gtgagcgtat tggtgaattg cacgaaggtg cattgctggt actttcacag ggacgagtcg
    25261 tcgatatcga tgacgcggtg gcacgtcatc tgcacggtgt gcggcaaggg attaatctac
    25321 cgttacggct ggaaggtgaa attgtcggcg taattggcct gacaggtgaa ccagagaatc
    25381 tgcgtaaata tggcgaactg gtctgcatga cggctgaaat gatgctggaa cagtcgcggt
    25441 tgatgcactt gttggctcag gatagccgtt tgcgggagga actggtgatg aacctgattc
    25501 aggcagagga gaatactccc gcacttactg aatgggcgca acggctgggg atcgatctca
    25561 atcaaccgcg agtggtggct attgttgagg tcgacagcgg tcagcttggt gtggacagcg
    25621 caatggcgga gttacaacaa ctgcaaaacg cgctgactac gcccgagcgt aataatctgg
    25681 tggcgattgt ctcgctaacc gaaatggtgg tgttgaaacc ggcgttgaac tcttttgggc
    25741 gctgggatgc agaagatcat cgtaagcgag ttgaacaact gattacccgc atgaaagagt
    25801 acggccagct gcgttttcgc gtttcactgg gcaactattt taccggtcct ggcagtattg
    25861 cccgatccta tcgtacggcg aaaacgacga tggtggtggg taaacagcgg atgccagaaa
    25921 gtcgctgcta tttttatcag gatctgatgt tacctgtgtt actcgacagt ttgcgtggcg
    25981 actggcaggc caacgaactg gcgcgaccgc tggcgcggct gaaagcgatg gacaataacg
    26041 gcttgctgcg acgaacgctg gcggcgtggt ttcgtcacaa tgtgcaaccg ctggcaacgt
    26101 caaaggcgtt gtttattcat cgtaatactc tggagtatcg gcttaatcgt atatcggaac
    26161 tgaccgagct tgatttgggt aattttgatg acaggttgct gctgtatgtg gcgttgcagc
    26221 tggatgaaga gcggtaggtt atgcgttaag gtcgaggcgg cgctggctca ttcccctcag
    26281 cccgaagggt agaggggaat gtccgagtta accgtcagtt cggcattatt tacgcgttag
    26341 tttttccaga tcggcttcaa tctcgctgat cttattggtt acgactgact ccaggtgacg
    26401 taagtcgtcg aggatcttac gcttaagatc aacttcgctg cggtcacgct ggcagatttg
    26461 atcaagctca tcaatgatat accgtagatt cgggctgatt tcctggactt ctttataacc
    26521 ctgacccaca ccatcagcga cgaccgtttt acgctgacgc ggatatttaa acttaacgct
    26581 cttggcgaaa aactcgcctt tgtctttctg gaaatagatt ttcagaatat cgttgttggc
    26641 ttcctgccgg aggctgtaac gatcaatttc ttcaggattg gtaataccca gacttttcag
    26701 attgtcgtac atagcgttac ctcaaaatga gtcagtaaat tgtgcttatt ttagcatttg
    26761 gcctggcccg cccggctcga ctgtgatcgc agaaagcctt tttcttccag acttctatca
    26821 tggcgtaaaa atcaaaaaat tacctgcttt attgtggcga tattccatgg gatggattag
    26881 cacatttatc ttcgttttgc gaattacatg acagtccgaa acaagatgaa aaagtattta
    26941 aaaaaggggg gaagaatgag taggtcgaat tattctgagc cagaaagcat acgggcagca
    27001 ttatgctgcc cgtatatgta ctcttaatcg atggtacgca gcagttcgtt aatgccgact
    27061 ttgccgcgag ttttcgcgtc aactttctta acgataaccg cacagtagag gctgtatttg
    27121 ccatctttcg acggcagatt acctgaaaca accacagacc ccgccggaac gcgaccgtag
    27181 tggatttcgc cggtttcacg gtcgtaaata cgggtgctct gaccaatgta tacgcccata
    27241 gagatgacgg aaccttcttc gacaatcacc ccttcaacca cttcggagcg tgcgccaatg
    27301 aagcagttat cttcaataat ggtcgggtta gcctgcagcg gctccagcac gccgccgatg
    27361 cccacgccac cggaaaggtg gacgttttta ccaatctgcg cacaagaacc gacggtcgcc
    27421 caggtatcaa ccatcgtgcc ttcatcaaca tatgcgccga tgttgacgta agacggcatc
    27481 agcacggtgt tacgggcaat aaatgcaccc tgacgtaccg ccgctggtgg cacaacgcgg
    27541 aagccttctt tctggaaacg tgcttcgtcg tagtcggcga atttcatcgg cactttgtcg
    27601 aagtagcggc tttctgcccc ttcgatcacc tgattatcat taatacggaa agagagcagc
    27661 accgcttttt tcaaccactg atgcgtcacc cactgaccgt caattttttc cgctacacgc
    27721 agtgcaccgg aatccagcag ggcgatcacc tgatttaccg cttcgcgggt aacggtgtct
    27781 gcgttggctg gcgtgatctc ggcacggcgt tcaaaagcgg tttcaataat gttctgtaac
    27841 tgctgcattg ttaaactctt ttcatatcag taaacacatc accctttatc gtttggattg
    27901 agggcctctg tcaaccgctg atgcacttcc tgctgcaact cgttattaag tgcacgccgg
    27961 tcagcggtgg caattatgaa taaatcttct actcgctcgc caatggttgt aattcgggca
    28021 ccatgaagcg aaattcccag atcggcaaaa attttcccga ctctcgccag cagtcctggc
    28081 tggtcgagcg cgataagctc aaggaacgat ttgcggtcgg tatgggtcgg caaaaacgtt
    28141 acttcggttt caacagtaaa atggcgtaat ttggcgggtt ggcgacgggg ctgcggtggc
    28201 tgccagctac tttgcgtcag tacttgctcc agaccaaacc gaataacctc atgacgatct
    28261 gcggacagcg ggctgccatc gggttccagc acgataaagg tatccatcgc cataccgtcg
    28321 cgagtggtga aaatttgtgc gtcgtgaaca cttaaattgc ggcggtctaa ttcggcacag
    28381 acggcggcaa acagataggg gcggtccggg ctccagataa aaatctcggt gcctccacgc
    28441 gtagcctgcg ggctaagcaa taccagcggt ttgcttaaat catgctgtaa taaatggcgg
    28501 gcatgccagg ccagttgatt tgggctatgg cggacaaaat agttagcacg acagcgtgac
    28561 caaatttggt gcagcgcctc ttcgtcgatg ttatccatgc gcagtagtgc cagtgcctgg
    28621 agttggtgat ggcgaacccg ttcgcgcata tccggcgtgt tttgcattcc gcgtcgtagc
    28681 tgcttttcgg tggcaaaata gagttcgcgc aacagacttt gcttccagct attccacagc
    28741 gtttcgttgg tggcgcaaat gtcagccact gtcaggcata ccagatagcg cagacgattt
    28801 tccgtttgaa cttcttcggc aaactgcttg atgacttcag ggtcctgaat atcgcggcgt
    28861 tgtgctgtca ccgacatcag caagtgctgg cgaaccagcc aggcaaccag ctgtgtttcg
    28921 cgtgagttca gcccgtggag ttctgcaaaa tgcactacat cctgagcacc gagaatggag
    28981 tggtcgccgc cgcgtccttt ggcgatatcg tgaaacagcg cggcgatgaa aatcagctca
    29041 gttgacggca ggcgcggcca gacgtccaca cacaacggat ggcgctggcg cgtttcttca
    29101 ctggcaaaac tctccagttt cagcatcacg cggatagtat gttcatccac cgtgtaggcg
    29161 tggaacagat caaactgcat ctgcccgacg atatgcgacc attgcggcat atacgcgccg
    29221 agcacgctat ggcgatgcat tggcaatagc ccgcgccgca ccgctccggg gtgacgcaga
    29281 atgctcaaaa acagttttcg tgcttccgga atattacaca gcggttgttg cagatggcga
    29341 cgggcatggc gtaactggcg cagcgtggtg gagtaaatgc cggtgatcgc actgttgcgc
    29401 accatggtgt agaacatacg caagatggct tccggctggc gcataaatag tgtttcatca
    29461 cgcaggtcga ttagcgtacc gcgtagctga aactcatcgt cgattggacg tggtttttcg
    29521 tcggcgggaa gggcgaggat ggcttcatcg aacagttgca gcagcatctg gttgagttca
    29581 ctgacgcggc gtgtaacgcg gaagtaatcc ttcatcatcc gctcgaccgg ttcgttgcct
    29641 tcaccactgt aattcagacg ctgagcgacg ctaagttggc gatcgaataa cagacgatta
    29701 tcgtaacggc tgacgaccag atgcagggca aagcgaatac gccacagtat atgcagacat
    29761 tcgtttaatt ccgcccgctc cgctgaggtt aagaagccaa acccaaccat ttcatccagc
    29821 gatgttgcgc caaaatgacg gcgggccacc cattgcagag tgtggatatc gcgcaagccg
    29881 ccagggctac ttttgatgtc tggttcaaga ttgtagctgg tgccgtggta acgctgatgg
    29941 cgctggttct gttcttcaac tttcgccgcg tagaacttgt cggaaggcca gaatccttcg
    30001 ctaaaaatat gtttttgcag ttcgaggaag agcgcaacat cgccaattaa taagcgggat
    30061 tcgattaaat tggtggcgac ggttaaatcc gataaccctt ccagcatgca ctcttcaagc
    30121 gtgcgcacac tatgaccgac ttccagcttt acatcccaga gcagcgttaa cagctcgccc
    30181 actttttgcg cctgatcgtc cgggagcttt ttacggctta aaatcagtaa atcgatgtca
    30241 gaaagtggat gcagctcgcc acgaccgtag ccaccgacgg cgaccaatgc caggtcggca
    30301 atctggctga atcccgcttc aatccataat cgttgcagga gctggtcgat aaactcggtg
    30361 cgcgcctcaa tcaactgttc tgcagagatc ccattgtcaa aggcatcacc cagccaacgc
    30421 tggaaagtat cgatatgggc ttttatccca ccgacggtta attcatcacg gggccagacg
    30481 catggatttt gcggttgacc gggcagggtg gggagagcgg tgtttgcgta ctgttctgga
    30541 agggtattca ttgtgcgcca cccataagat taaattatcg cattaaaaaa gccggcattc
    30601 gccggcttca tcttattcgt cgtgcgagat tatcgccggg atggtgtcat ccttgcgtag
    30661 cgtcaaaatt tcgcagccgt tatcagtcac cacaatagta tgctcatatt gtgcagacaa
    30721 gctgcgatct ttggttttta ccgtccagcc atctttcatg gtgcggatct cttttttacc
    30781 cgcgttgacc attggctcaa tggtgaacgt catccccggt ttcagcacga cgttggtttc
    30841 acgggagtca tagtgcagca cctgcggttc ttcatggaag ccgcgaccaa taccgtgtcc
    30901 acaatattca cgaacgacgg agaagccttc tgcttcgaca aacttctgaa tcgccgcacc
    30961 gatttcgcgc aggttaatgc ctggtttgac catgcgcagc gccaggtaca ggctttcttg
    31021 cgtgatgcgg cacagacgtt cgcccatgat ggtcggctta ccgacgataa acattttcga
    31081 ggtatcgccg tggaaaccat ctttgattac ggtgacatca atgttaacga tatcgccatc
    31141 tttcagcagc ttagcatcgt ccgggatacc gtggcacacc acttcattaa tagagatgca
    31201 aacggatttc ggatagccgt gatagccgag gcaggcagaa accgcgtgtt gttcattaac
    31261 aatgtaatca ttacagatgc gatccagctc gccggtgctg acgcccggtt taacatacgg
    31321 ttcgatcatc tccagcactt cggcagccag tcggccagcg acgcgcattt tttcgatatc
    31381 ttctggggtc ttgattgaga tagccattaa ttctgtccat cagcgtcggt gataccgaca
    31441 atatatatgt aagtgccgtc aatggtatca cacccgggca aattgagaat cattctgaat
    31501 ttcgccaaac gtgccactga aggttttcta taatagaaaa ttcgacatct gatgctgtac
    31561 acagcgccaa caattattgg tgtccatgac gtatttgtgg tataaagcgc gccggacttc
    31621 cgatccattt cgtatacaca gactggacgg aagcgacaat ctcactttgt gtaacaacac
    31681 acacgtatcg gcacatattc cggggtgccc tttggggtcg gtaatatggg atacgtggag
    31741 gcataacccc aacttttata tagaggtttt aatcatggca actgtttcca tgcgcgacat
    31801 gctcaaggct ggtgttcact tcggtcacca gacccgttac tggaacccga aaatgaagcc
    31861 gttcatcttc ggtgcgcgta acaaagttca catcatcaac cttgagaaaa ctgtaccgat
    31921 gttcaacgaa gctctggctg aactgaacaa gattgcttct cgcaaaggta aaatcctttt
    31981 cgttggtact aaacgcgctg caagcgaagc ggtgaaagac gctgctctga gctgcgacca
    32041 gttcttcgtg aaccatcgct ggctgggcgg tatgctgact aactggaaaa ccgttcgtca
    32101 gtccatcaaa cgtctgaaag acctggaaac tcagtctcag gacggtactt tcgaaaagct
    32161 gaccaagaaa gaagcgctga tgcgcactcg tgagctggag aaactggaaa acagcctggg
    32221 cggtatcaaa gacatgggcg gtctgccgga cgctctgttt gtaatcgatg ctgaccacga
    32281 acacattgct atcaaagaag caaacaacct gggtattccg gtatttgcta tcgttgatac
    32341 caactctgat ccggacggtg ttgacttcgt tatcccgggt aacgacgacg caatccgtgc
    32401 tgtgaccctg tacctgggcg ctgttgctgc aaccgtacgt gaaggccgtt ctcaggatct
    32461 ggcttcccag gcggaagaaa gcttcgtaga agctgagtaa taaggcttga taactccccc
    32521 aaaatagttc gagttgcaga aaggcggcaa gctcgagaat tcccgggagc ttacatcagt
    32581 aagtgaccgg gatgagcgag cgaagataac gcatctgcgg cgcgaaatat gaagggggag
    32641 agcccttata gaccaggtag tacacgtttg gttagggggc ctgcatatgg cccccttttt
    32701 cacttttata tctgtgcggt ttaatgccgg gcagatcaca tctccgagga ttttagaatg
    32761 gctgaaatta ccgcatccct ggtaaaagag ctgcgtgagc gtactggcgc aggcatgatg
    32821 gattgcaaaa aagcactgac tgaagctaac ggcgacatcg agctggcaat cgaaaacatg
    32881 cgtaagtccg gtgctattaa agcagcgaaa aaagcaggca acgttgctgc tgacggcgtg
    32941 atcaaaacca aaatcgacgg caactacggc atcattctgg aagttaactg ccagactgac
    33001 ttcgttgcaa aagacgctgg tttccaggcg ttcgcagaca aagttctgga cgcagctgtt
    33061 gctggcaaaa tcactgacgt tgaagttctg aaagcacagt tcgaagaaga acgtgttgcg
    33121 ctggtagcga aaattggtga aaacatcaac attcgccgcg ttgctgcgct ggaaggcgac
    33181 gttctgggtt cttatcagca cggtgcgcgt atcggcgttc tggttgctgc taaaggcgct
    33241 gacgaagagc tggttaaaca catcgctatg cacgttgctg caagcaagcc agaattcatc
    33301 aaaccagaag acgtatccgc tgaagtggta gaaaaagaat accaggtaca gctggatatc
    33361 gcgatgcagt ctggtaagcc gaaagaaatc gcagagaaaa tggttgaagg ccgcatgaag
    33421 aaattcaccg gcgaagtttc tctgaccggt cagccgttcg ttatggaacc aagcaaaact
    33481 gttggtcagc tgctgaaaga gcataacgct gaagtgactg gcttcatccg cttcgaagtg
    33541 ggtgaaggca tcgagaaagt tgagactgac tttgcagcag aagttgctgc gatgtccaag
    33601 cagtcttaat tatcgaaaag gagccgcctg agggcggctt ctttttgtgc ccatcttgta
    33661 aattcagcta acccttgtgg ggctgcgctg aaaagcgacg tacaatgtcg ctagtattaa
    33721 ttcatttcaa tcgttgacag tctcaggaaa gaaacatggc taccaatgca aaacccgtct
    33781 ataaacgcat tctgcttaag ttgagtggcg aagctctgca gggcactgaa ggcttcggta
    33841 ttgatgcaag catactggat cgtatggctc aggaaatcaa agaactggtt gaactgggta
    33901 ttcaggttgg tgtggtgatt ggtgggggta acctgttccg tggcgctggt ctggcgaaag
    33961 cgggtatgaa ccgcgttgtg ggcgaccaca tggggatgct ggcgaccgta atgaacggcc
    34021 tggcaatgcg tgatgcactg caccgcgcct atgtgaacgc tcgtctgatg tccgctattc
    34081 cattgaatgg cgtgtgcgac agctacagct gggcagaagc tatcagcctg ttgcgcaaca
    34141 accgtgtggt gatcctctcc gccggtacag gtaacccgtt ctttaccacc gactcagcag
    34201 cttgcctgcg tggtatcgaa attgaagccg atgtggtgct gaaagcaacc aaagttgacg
    34261 gcgtgtttac cgctgatccg gcgaaagatc caaccgcaac catgtacgag caactgactt
    34321 acagcgaagt gctggaaaaa gagctgaaag tcatggacct ggcggccttc acgctggctc
    34381 gtgaccataa attaccgatt cgtgttttca acatgaacaa accgggtgcg ctgcgccgtg
    34441 tggtaatggg tgaaaaagaa gggactttaa tcacggaata attcccgtga tggataaata
    34501 agggtaagat tccgcgtaag tatcgcgggg gcgttagtct ggttataagg cgttattgtt
    34561 gcaggcagtt tggtcacggc cagcgcgcag caaccggagc gtacaaaagt acgtgaggat
    34621 ggcgagcact gcccggggcc aaaatggcaa ataaaatagc ctaataatcc agacgattac
    34681 ccgtaatatg tttaatcagg gctatactta gcacacttcc actgtgtgtg actgtctggt
    34741 ctgactgaga caagttttca aggattcgta acgtgattag cgatatcaga aaagatgctg
    34801 aagtacgcat ggacaaatgc gtagaagcgt tcaaaaccca aatcagcaaa atacgcacgg
    34861 gtcgtgcttc tcccagcctg ctggatggca ttgtcgtgga atattacggc acgccgacgc
    34921 cgctgcgtca gctggcaagc gtaacggtag aagattcccg tacactgaaa atcaacgtgt
    34981 ttgatcgttc aatgtctccg gccgttgaaa aagcgattat ggcgtccgat cttggcctga
    35041 acccgaactc tgcgggtagc gacatccgtg ttccgctgcc gccgctgacg gaagaacgtc
    35101 gtaaagatct gaccaaaatc gttcgtggtg aagcagaaca agcgcgtgtt gcagtacgta
    35161 acgtgcgtcg tgacgcgaac gacaaagtga aagcactgtt gaaagataaa gagatcagcg
    35221 aagacgacga tcgccgttct caggacgatg tacagaaact gactgatgct gcaatcaaga
    35281 aaattgaagc ggcgctggca gacaaagaag cagaactgat gcagttctga tttcttgaac
    35341 gacaaaaacg ccgctcagta gatccttgcg gatcggctgg cggcgttttg ctttttattc
    35401 tgtctcaact ctggatgttt catgaagcaa ctcaccattc tgggctcgac cggctcgatt
    35461 ggttgcagca cgctggacgt ggtgcgccat aatcccgaac acttccgcgt agttgcgctg
    35521 gtggcaggca aaaatgtcac tcgcatggta gaacagtgcc tggaattctc tccccgctat
    35581 gccgtaatgg acgatgaagc gagtgcgaaa cttcttaaaa cgatgctaca gcaacagggt
    35641 agccgcaccg aagtcttaag tgggcaacaa gccgcttgcg atatggcagc gcttgaggat
    35701 gttgatcagg tgatggcagc cattgttggc gctgctgggc tgttacctac gcttgctgcg
    35761 atccgcgcgg gtaaaaccat tttgctggcc aataaagaat cactggttac ctgcggacgt
    35821 ctgtttatgg acgccgtaaa gcagagcaaa gcgcaattgt taccggtcga tagcgaacat
    35881 aacgccattt ttcagagttt accgcaacct atccagcata atctgggata cgctgacctt
    35941 gagcaaaatg gcgtggtgtc cattttactt accgggtctg gtggcccttt ccgtgagacg
    36001 ccattgcgcg atttggcaac aatgacgccg gatcaagcct gccgtcatcc gaactggtcg
    36061 atggggcgta aaatttctgt cgattcggct accatgatga acaaaggtct ggaatacatt
    36121 gaagcgcgtt ggctgtttaa cgccagcgcc agccagatgg aagtgctgat tcacccgcag
    36181 tcagtgattc actcaatggt gcgctatcag gacggcagtg ttctggcgca gctgggggaa
    36241 ccggatatgc gtacgccaat tgcccacacc atggcatggc cgaatcgcgt gaactctggc
    36301 gtgaagccgc tcgatttttg caaactaagt gcgttgacat ttgccgcacc ggattatgat
    36361 cgttatccat gcctgaaact ggcgatggag gcgttcgaac aaggtcaggc agcgacgaca
    36421 gcattgaatg ccgcaaacga aatcaccgtt gctgcttttc ttgcgcaaca aatccgcttt
    36481 acggatatcg ctgcgttgaa tttatcggta ctggaaaaaa tggatatgcg cgaaccacaa
    36541 tgtgtggacg atgtgttatc tgttgatgcg aacgcgcgtg aagtcgccag aaaagaggtg
    36601 atgcgtctcg caagctgagg ataatccggc tacagagagt cgcgctattt gttagcgtag
    36661 ggcttcagtg atatagtctg cgccatctga tcgtaagtag ttggctttat aaggtcaggt
    36721 atgccgtggt tttacacggc ttttttttgt ataggcttca gtattcctga gtaccgtaaa
    36781 ccctgtcagg gaataaaaaa cgcgtgatgt tgtctgctac tcaaccactt agcgaaaaat
    36841 tgccagcgca tggctgccgt catgttgcga tcattatgga cggcaatggt cgctgggcaa
    36901 aaaagcaagg gaagattcgt gcctttgggc ataaagccgg ggcaaaatcc gtccgccggg
    36961 ctgtctcttt tgcggccaac aacggtattg aggcgttaac gctgtatgcc tttagtagtg
    37021 aaaactggaa ccgaccagcg caggaagtca gtgcgttaat ggaactgttt gtgtgggcgc
    37081 tcgatagcga agtaaaaagt ctgcaccgac ataacgtgcg tctgcgtatt attggcgata
    37141 ccagtcgctt taactcgcgt ttgcaagaac gtattcgtaa atctgaagcg ctaacagccg
    37201 ggaataccgg tctgacgctg aatattgcgg cgaactacgg tggacgttgg gatatagtcc
    37261 agggagtcag gcaactggct gaaaaggtgc agcaaggaaa cctgcaacca gatcagatag
    37321 atgaagagat gctaaaccag catgtctgta tgcatgaact ggcccctgta gatttagtaa
    37381 ttaggactgg gggggagcat cgcattagta actttttgct ttggcaaatt gcctatgccg
    37441 aactttactt tacagatgtt ctctggcccg atttcgatga acaagacttt gaaggggcgt
    37501 taaatgcctt tgctaatcga gagcgtcgtt tcggcggcac cgagcccggt gatgaaacag
    37561 cctgatgggg gtcgcttttg ctgaagtatc gcctgatatc tgcttttgtg ttaatacccg
    37621 tcgtcatcgc ggcgttgttt ctgttgccgc cggtggggtt cgccatcgta acgctggtgg
    37681 tctgcatgct ggcagcgtgg gaatggggac agcttagcgg ttttaccact cgttcgcagc
    37741 gagtatggtt ggcggtgtta tgcgggttat tgttggcgct gatgcttttt ctgttgccgg
    37801 aatatcaccg aaatattcat caaccgctgg ttgaaatctc actttgggct tcgctgggtt
    37861 ggtggattgt cgcgctattg ctggtgctgt tttacccagg ttccgcagca atctggcgta
    37921 actctaaaac attgcgcctt atttttggcg tgctaaccat tgttcccttc ttctggggca
    37981 tgctggcgtt acgggcctgg cactacgacg agaatcatta cagtggcgca atatggctgc
    38041 tctatgtcat gatcctggta tggggcgctg actctggcgc atatatgttt ggcaaattgt
    38101 ttggtaaaca taagctggca ccgaaggttt ctccgggtaa aacctggcaa ggctttatag
    38161 gtggactagc gaccgcagcg gtaatctcat ggggttatgg catgtgggcg aatctcgacg
    38221 tcgctcccgt caccttactc atttgctcta ttgtcgcagc gttagcctca gtgctcggcg
    38281 atctgaccga gagtatgttt aagcgtgaag caggaattaa ggacagcggt catttaattc
    38341 caggacacgg tggtatttta gatcgtattg atagcctgac ggctgcggta ccggtctttg
    38401 cttgcttgtt gttactggta ttcaggacgc tttaacggaa ggtaatatgc tgagttttct
    38461 ctgggatttg gcttcgttca tcgttgcact gggtgtactt atcaccgtgc atgaatttgg
    38521 tcatttctgg gttgcccggc gttgtggtgt tcgcgttgag cgtttctcaa tagggtttgg
    38581 taaggcgctc tggcggcgaa ctgataagct cggcaccgaa tatgttatcg ccctgatccc
    38641 gctgggcggt tatgtcaaaa tgctggatga gcgcgcagaa ccggtcgttc cggaactccg
    38701 ccaccatgcc ttcaataata aatctgttgg ccaacgagcg gcgattattg ccgcaggtcc
    38761 ggttgcaaac ttcatttttg ctatctttgc ctactggctg gtttttatta ttggtgtgcc
    38821 tggcgtacgt ccggtggttg gtgaaatagc agccaattcg atagctgcgg aagcacaaat
    38881 tgcaccaggt acggaactaa aagccgtaga tggtatcgaa acgcctgatt gggatgccgt
    38941 gcgtttgcag ttggtcgata aaattggcga tgaaagcacc accattacag tagcgccatt
    39001 tggcagcgat caacggcggg atgtaaagct cgatttacgt cactgggcgt ttgagcctga
    39061 taaagaagat ccggtatctt cgctggggat tcgtcctcgt gggccgcaaa ttgaacctgt
    39121 actggaaaat gtgcagccaa actcggcggc aagcaaggca ggtttgcaag caggcgacag
    39181 gatcgttaaa gtcgatggtc agcccttaac gcagtgggtg acctttgtga tgcttgtccg
    39241 ggataacccg ggtaaatcct tagcgttaga aatcgaaagg cagggaagtc ccttgtcttt
    39301 gacattaatc ccggagagta aaccgggtaa tggtaaagcg attggttttg tcggtattga
    39361 gccgaaagtc attcctttgc cagatgagta taaagttgta cgccagtatg ggccgttcaa
    39421 cgccatcgtc gaagccacgg acaaaacgtg gcagctgatg aagctgacgg tcagtatgct
    39481 gggaaaattg atcaccggtg atgtgaaact gaacaacctc agtgggccga tctctatcgc
    39541 caagggggct gggatgacag cggaactcgg ggtagtttat tacctgccgt ttcttgcgct
    39601 tattagcgtg aacttaggga taattaacct gtttccgttg cccgtacttg acggggggca
    39661 tctgctgttc cttgcgatcg aaaagatcaa gggcggaccg gtatccgagc gggttcaaga
    39721 cttttgttat cgcattggct cgattctgct ggtgctgtta atggggcttg cacttttcaa
    39781 tgatttctct cggttatgag agttagttag gaagaacgca taataacgat ggcgatgaaa
    39841 aagttgctca tagcgtcgct gctgtttagc agcgccaccg tatacggtgc tgaagggttc
    39901 gtagtgaaag atattcattt cgaaggcctt cagcgtgtcg ccgttggtgc ggccctcctc
    39961 agtatgccgg tgcgcacagg cgacacggtt aatgatgaag atatcagtaa taccattcgc
    40021 gctctgtttg ctaccggcaa ctttgaggat gttcgcgtcc ttcgtgatgg tgataccctt
    40081 ctggttcagg taaaagaacg tccgaccatt gccagcatta ctttctccgg taacaaatcg
    40141 gtgaaagatg acatgctgaa gcaaaacctc gaggcttctg gtgtgcgtgt gggcgaatcc
    40201 ctcgatcgca ccaccattgc cgatatcgag aaaggtctgg aagacttcta ctacagcgtc
    40261 ggtaaatata gcgccagcgt aaaagctgtc gtgaccccgc tgccgcgcaa ccgtgttgac
    40321 ctaaaactgg tgttccagga aggtgtgtca gctgaaatcc agcaaattaa cattgttggt
    40381 aaccatgctt tcaccaccga cgaactgatc tctcatttcc aactgcgtga cgaagtgccg
    40441 tggtggaacg tggtaggcga tcgtaaatac cagaaacaga aactggcggg cgaccttgaa
    40501 accctgcgca gctactatct ggatcgcggt tatgcccgtt tcaacatcga ctctacccag
    40561 gtcagtctga cgccagataa aaaaggtatt tacgtcacgg tgaacatcac cgaaggcgat
    40621 cagtacaagc tttctggcgt tgaagtgagc ggcaaccttg ccgggcactc cgctgaaatt
    40681 gagcagctga ctaagatcga gccgggtgag ctgtataacg gcaccaaagt gaccaagatg
    40741 gaagatgaca tcaaaaagct tctcggtcgc tatggttatg cctatccgcg cgtacagtcg
    40801 atgcccgaaa ttaacgatgc cgacaaaacc gttaaattac gtgtgaacgt tgatgcgggt
    40861 aaccgtttct acgtgcgtaa gatccgtttt gaaggtaacg atacctcgaa agatgccgtc
    40921 ctgcgtcgcg aaatgcgtca gatggaaggt gcatggctgg ggagcgatct ggtcgatcag
    40981 ggtaaggagc gtctgaatcg tctgggcttc tttgaaactg tcgataccga tacccaacgt
    41041 gttccgggta gcccggacca ggtagatgta gtctacaagg tgaaagaacg taacaccggt
    41101 agcttcaact tcgggattgg ttacggtact gaaagtggcg tgagcttcca ggctggtgtg
    41161 cagcaggata actggttagg tacaggttat gctgttggta tcaacgggac caaaaacgat
    41221 taccagacct atgctgaact gtcggtaacc aacccgtact tcactgtaga tggtgtaagc
    41281 ctcggtggtc gtctcttcta taatgacttc caggcagatg acgccgacct gtccgactat
    41341 accaacaaga gttatggtac agacgtgacg ttgggcttcc cgatcaacga atataactcg
    41401 ctgcgtgcag gtctgggtta tgtacataac tccctgtcca acatgcagcc tcaggttgcg
    41461 atgtggcgtt atctgtactc tatgggtgaa catccgagca cctctgatca ggataatagc
    41521 ttcaaaacgg acgacttcac gttcaactat ggttggacct ataacaagct tgaccgtggt
    41581 tacttcccga cagatggttc acgtgtcaac ctgaccggta aagtgaccat tcctggatcg
    41641 gataacgaat actacaaagt gacgttagac acggcgactt atgtgccgat cgatgacgat
    41701 cacaaatggg ttgttctggg gcgtacccgc tggggttatg gtgatggttt aggcggcaaa
    41761 gagatgccgt tctacgagaa cttctatgcc ggtggttcca gcaccgtgcg tggcttccag
    41821 tccaatacaa ttggtccgaa agcagtttac ttcccgcatc aggccagtaa ttatgatccg
    41881 gactatgatt acgaatgtgc gactcaggac ggcgcgaaag acctgtgtaa atcggatgat
    41941 gctgtaggcg gtaacgccat ggcggttgcc agcctcgagt tcatcacccc gacgccgttt
    42001 attagcgata agtatgctaa ctcggttcgt acttccttct tctgggatat gggtaccgtt
    42061 tgggatacaa actgggattc cagccaatat tctggatatc cggactatag tgatccaagc
    42121 aatatccgta tgtctgcggg tatcgcatta caatggatgt ccccattggg gccgttggtg
    42181 ttctcctacg cccagccgtt caaaaagtac gatggagaca aggcagaaca gttccagttt
    42241 aacatcggta aaacctggta agtgttctcc acaaaggaat gtagtggtag tgtagcgatg
    42301 actttaggcg atcaatataa gatcgccggg ccacgcaaag aactgcaccc tccggtgcaa
    42361 atgggatggt aaggagttta ttgtgaaaaa gtggttatta gctgcaggtc tcggtttagc
    42421 actggcaact tctgctcagg cggctgacaa aattgcaatc gtcaacatgg gcagcctgtt
    42481 ccagcaggta gcgcagaaaa ccggtgtttc taacacgctg gaaaatgagt tcaaaggccg
    42541 tgccagcgaa ctgcagcgta tggaaaccga tctgcaggct aaaatgaaaa agctgcagtc
    42601 catgaaagcg ggcagcgatc gcactaagct ggaaaaagac gtgatggctc agcgccagac
    42661 ttttgctcag aaagcgcagg cttttgagca ggatcgcgca cgtcgttcca acgaagaacg
    42721 cggcaaactg gttactcgta tccagactgc tgtgaaatcc gttgccaaca gccaggatat
    42781 cgatctggtt gttgatgcaa acgccgttgc ttacaacagc agcgatgtaa aagacatcac
    42841 tgccgacgta ctgaaacagg ttaaataagt aatgccttca attcgactgg ctgatttagc
    42901 gcagcagttg gatgcagaac tacacggtga tggcgatatc gtcatcaccg gcgttgcgtc
    42961 catgcaatct gcacaaacag gtcacattac gttcatggtt aacccaaaat accgtgagca
    43021 tttaggcttg tgccaggcgt ccgcggttgt catgacccag gacgatcttc ctttcgcgaa
    43081 aagtgccgcg ctggtagtga agaatcccta cctgacttac gcgcgcatgg cgcaaatttt
    43141 agataccacg ccgcagcccg cgcagaacat tgcacccagt gcggtgatcg acgcgacggc
    43201 aaagctgggt aacaacgtat cgattggcgc taacgcggtg attgagtccg gcgttgaact
    43261 gggcgataac gtgattatcg gtgccggttg cttcgtaggt aaaaacagca aaatcggtgc
    43321 aggttcgcgt ctctgggcga acgtaaccat ttaccatgag atccagatcg gtcagaattg
    43381 cctgatccag tccggaacag tggtaggcgc agacggcttt ggttatgcca acgatcgtgg
    43441 taactgggtg aagatcccac agattggtcg cgtaattatt ggcgatcgcg tggagatcgg
    43501 tgcctgcaca accatcgatc gcggcgcgct ggatgacact gttattggca atggcgtgat
    43561 cattgataac cagtgccaga ttgcacataa cgtcgtgatt ggcgacaata cggcggttgc
    43621 cggtggcgtc attatggcgg gcagcctgaa aattggtcgt tactgcatga tcggcggagc
    43681 cagcgtaatc aacgggcata tggaaatatg cgacaaagtg acggttacgg gcatgggtat
    43741 ggtgatgcgt cccatcactg aaccaggcgt ctattcctca ggcattccgc tgcaacccaa
    43801 caaagtctgg cgcaaaaccg ctgcactggt gatgaacatt gatgacatga gcaagcgtct
    43861 gaaatcgctt gagcgcaagg ttaatcaaca agactaacgt tccatctttt gttcgccaaa
    43921 ctttacggcc tgtctcattc ttacgattgc ggcaggccgt gttattattg tcgtttctta
    43981 tattttgaca ggaagagtat cttgactact aacactcata ctctgcagat tgaagagatt
    44041 ttagaacttc tgccgcaccg tttcccgttc ttactggtgg atcgcgtgct ggattttgaa
    44101 gaaggtcgtt ttctgcgcgc agtaaaaaat gtctctgtca atgagccatt cttccagggc
    44161 catttccctg gaaaaccgat tttcccgggt gtgctgattc tggaagcaat ggcacaggca
    44221 acaggtattc tggcgtttaa aagcgtagga aaactggaac cgggtgagct gtactacttc
    44281 gctggtattg acgaagcgcg cttcaagcgc ccggtcgtgc ctggcgatca aatgatcatg
    44341 gaagtcactt tcgaaaaaac gcgccgcggc ctgacccgtt ttaaaggggt tgctctggtc
    44401 gatggtaaag tagtttgcga agcaacgatg atgtgtgctc gtagccggga ggcctgataa
    44461 gtgattgata aatccgcctt tgtgcatcca accgccattg tggaagaggg cgcgtcaatt
    44521 ggcgcgaacg cacacattgg tcctttttgt atcgttggac cccatgtcga aattggtgag
    44581 ggtaccgtac tgaaatctca cgttgtcgtg aatggtcata ctaaaattgg ccgcgataat
    44641 gagatttatc agttcgcctc catcggcgaa gttaaccagg atctgaaata tgctggcgaa
    44701 ccgacccgtg tggaaatcgg cgatcgtaac cgcattcgcg aaagcgtcac cattcatcgt
    44761 ggcacagtcc agggcggtgg attgacgaag gtgggcagcg acaacttact gatgatcaac
    44821 gcgcacattg cgcacgattg tacggtaggt aaccgctgta ttctcgccaa caacgcaacg
    44881 ctggcgggtc acgtatcggt tgacgacttc gcgatcatcg gcggcatgac cgcagtccat
    44941 cagttctgca tcattggtgc gcacgtgatg gttggcggct gctccggtgt ggcgcaggac
    45001 gtccctcctt atgtcattgc gcagggtaac cacgcaacgc cgttcggtgt caatatcgaa
    45061 gggctgaagc gccgcggatt cagccgtgag gcgattaccg ctatccgcaa tgcgtataag
    45121 ctgatttatc gtagcggtaa aacgctcgat gaagtgaaac cggaaattgc tgaactggcg
    45181 gaaacatatc cggaagtgaa agcctttacc gatttctttg cacgctcaac gcgcggtctg
    45241 attcgttaat gactgaacag cgtccattaa cgattgccct ggtcgccgga gaaacctccg
    45301 gcgatatcct gggggccggt ttaatccgcg ctctgaaaga acgtgtgccc aacgcccgct
    45361 ttgttggtgt tgccgggcca cgaatgcagg ctgaaggctg cgaagcctgg tacgaaatgg
    45421 aagaactggc ggtgatgggc attgttgaag tgctcggtcg tctgcgtcgc ttactgcata
    45481 ttcgtgccga tctgacaaag cgttttggcg aactgaagcc agatattttt gttggtattg
    45541 atgcgcctga cttcaatatt actcttgaag gtaacctcaa aaagcagggt atcaaaacca
    45601 ttcattacgt cagtccatcc gtctgggcgt ggcgacagaa acgcgttttc aaaataggca
    45661 gagccaccga tctggtgctc gcatttctgc ctttcgaaaa agcgttttat gacaaataca
    45721 atgtaccgtg ccgctttatc ggtcatacca tggctgatgc catgccgtta gatccagata
    45781 aaaatgccgc ccgtgatgtg ctggggatcc ctcacgatgc ccactgcctg gcgttgctac
    45841 cagggagccg tggtgcagaa gttgaaatgc ttagtgccga tttcctgaaa acggcccagc
    45901 ttttgcgcca gacatatccg gatctcgaaa tcgtggtgcc actggtgaat gccaaacgcc
    45961 gcgagcagtt tgaacgcatc aaagctgaag tcgcgccaga cctttcagtt catttgctgg
    46021 atgggatggg ccgtgaggcg atggtcgcca gcgatgcggc gctactggcg tcgggtacgg
    46081 cagccctgga gtgtatgctg gcgaaatgcc cgatggtggt gggatatcgc atgaagcctt
    46141 ttaccttctg gttggcgaag cggctggtga aaactgatta tgtctcgctg ccaaatctgc
    46201 tggcgggcag agagttagtc aaagaattat tgcaggaaga gtgtgagccg caaaaactgg
    46261 ctgcggcgct gttaccgctg ttggcgaacg ggaaaaccag ccacgcgatg cacgatacct
    46321 tccgtgaact gcatcagcag atccgctgca atgccgatga gcaggcggca caagccgttc
    46381 tggagttagc acaatgatcg aatttgttta tccgcacacg cagctggttg cgggtgtgga
    46441 tgaagtcgga cgtgggccgt tagttggcgc ggtcgtcacc gctgcggtga tccttgaccc
    46501 ggcgcgcccg attgccgggc tgaatgattc caaaaagctg agcgaaaaac gccgtctggc
    46561 gctctatgaa gagatcaaag agaaagcgtt gagctggagt ctgggccgcg cggaacccca
    46621 cgaaatcgac gagctgaaca ttcttcatgc gaccatgctg gcgatgcagc gtgccgtcgc
    46681 tgggctgcat attgcgccgg aatatgtgtt gattgatggt aaccgctgcc cgaaattacc
    46741 gatgcctgcg atggctgtgg tgaaaggcga tagccgcgta ccggaaatca gtgccgcgtc
    46801 tatcctggcg aaagtgacgc gtgacgccga aatggcggcg ctggatattg ttttcccgca
    46861 atatggtttt gcccaacaca aagggtaccc aaccgctttt catctggaaa aactggctga
    46921 acacggcgcg accgaacacc atcggcgcag ctttgggcct gtcaaacgcg cactgggact
    46981 tgcgtcctga ttcttgtgtc gagattaagt aaaccggaat ctgaagatgt ctgaaccacg
    47041 tttcgtacac ctgcgggtgc acagcgacta ctcgatgatc gatggcctgg ccaaaaccgc
    47101 accgttggta aaaaaggcgg cggcgttggg tatgccagca ctggcgatca ccgatttcac
    47161 caacctttgt ggtctggtga agttctacgg agcgggacat ggcgcaggga ttaagcctat
    47221 cgtcggggca gattttaacg tccagtgcga cctgctgggt gatgagttaa cccacctgac
    47281 ggtactggcg gcgaacaata ccggctatca gaatctgacg ttgctgatct caaaagcgta
    47341 tcagcgcggg tacggtgccg ccgggccgat catcgatcgc gactggctta tcgaattaaa
    47401 cgaagggttg atccttcttt ccggcggacg catgggcgac gtcggacgca gtcttttgcg
    47461 tggtaacagc gcgctggtag atgagtgtgt cgcgttttat gaagaacact tcccggatcg
    47521 ctattttctt gagctgatcc gcaccggcag gccggatgaa gaaagctatc tgcacgcggc
    47581 ggtggaactg gcggaagcgc gcggtttgcc cgtcgtggcg accaacgacg tgcgctttat
    47641 cgacagcagc gactttgacg cacacgaaat ccgcgtcgcg atccacgacg gctttaccct
    47701 cgacgatcct aaacgcccgc gtaactattc gccgcagcaa tatatgcgta gcgaagagga
    47761 gatgtgtgag ctgtttgccg acatccccga agcccttgcc aacaccgttg agatcgccaa
    47821 acgctgtaac gtaaccgtgc gtcttggtga atacttcctg ccgcagttcc cgaccgggga
    47881 catgagcacc gaagattatc tggtcaagcg tgcaaaagag ggcctggaag agcgtctggc
    47941 ctttttattc cctgatgagg aagaacgtct taagcgccgc ccggaatatg acgaacgtct
    48001 ggagactgaa cttcaggtta tcaaccagat gggcttcccg ggctacttcc tcatcgttat
    48061 ggaatttatc cagtggtcga aagataacgg cgtaccggta gggccaggcc gtggctccgg
    48121 tgcgggttca ctggtggcct acgcgctgaa aatcaccgac ctcgatccgc tggaatttga
    48181 cctgctgttc gaacgtttcc ttaacccgga acgtgtctcc atgcctgact tcgacgttga
    48241 cttctgtatg gagaaacgcg atcaggttat cgagcacgta gcggacatgt acggtcgtga
    48301 tgcggtatcg cagattatca ccttcggtac gatggcggcg aaagcggtga tccgcgacgt
    48361 aggccgcgtg ctggggcatc cgtacggctt tgtcgatcgt atctcgaaac tgatcccgcc
    48421 cgatccgggg atgacgctgg cgaaagcgtt tgaagccgag ccgcagctgc cggaaatcta
    48481 cgaagcggat gaagaagtta aggcgctgat cgacatggcg cgcaaactgg aaggggtcac
    48541 ccgtaacgcc ggtaagcacg ccggtggggt ggttatcgca ccgaccaaaa ttaccgattt
    48601 tgcgccgctt tattgcgatg aagagggcaa acatccggtc acccagtttg ataaaagcga
    48661 cgttgaatac gccggactgg tgaagttcga cttccttggt ttgcgtacgc tcaccatcat
    48721 caactgggcg ctggagatga tcaacaagcg gcgggcgaag aatggcgagc cgccgctgga
    48781 tatcgccgcg atcccgctgg atgacaagaa aagcttcgac atgctgcaac gctcggaaac
    48841 cactgcggta ttccagcttg aatcgcgcgg catgaaggac ctgatcaagc gtctgcaacc
    48901 tgactgcttc gaagatatga tcgccctggt ggcactgttc cgccccggtc cgttgcaatc
    48961 agggatggtg gataacttta tcgaccgtaa acatggtcgt gaagagatct cctatccgga
    49021 cgtgcagtgg cagcatgaaa gcttgaaacc ggtactggag ccaacctacg gcatcatcct
    49081 gtatcaggaa caggtcatgc agattgccca ggtgctttct ggttataccc tcggtggcgc
    49141 ggatatgctg cgtcgtgcga tggggaagaa aaagccggaa gagatggcta agcaacgttc
    49201 tgtatttgct gaaggtgcag aaaagaacgg aatcaacgct gaactggcga tgaaaatctt
    49261 cgacctggtg gagaaattcg ctggttacgg atttaacaaa tcgcactctg cggcctatgc
    49321 tttggtgtca tatcaaacgt tatggctgaa agcacactat cctgcggagt ttatggcggc
    49381 ggtaatgacc gccgatatgg acaacaccga gaaggtggtg ggtctggtgg atgagtgctg
    49441 gcggatgggg ctgaaaatcc tgccaccaga tataaactcc ggtctttacc atttccacgt
    49501 caacgacgac ggcgaaatcg tgtatggtat tggcgcgatc aaaggggtcg gtgaaggtcc
    49561 gattgaggcc atcatcgaag cccgtaataa aggcggctac ttccgcgaac tgtttgatct
    49621 ctgcgcccgt accgacacca aaaagttgaa ccgtcgcgtg ctggaaaaac tgatcatgtc
    49681 cggggcgttt gaccgtcttg ggccacatcg cgcggcgctg atgaactcgc tgggtgatgc
    49741 gttaaaagcg gcagatcaac acgcgaaagc ggaagctatc ggtcaggccg atatgttcgg
    49801 cgtgctggcc gaagagccgg aacaaattga acaatcctac gccagctgcc aaccgtggcc
    49861 ggagcaggtg gtattagatg gggaacgtga aacgttaggc ctgtacctga ccggacaccc
    49921 tatcaaccag tatttaaaag agattgagcg ttatgtcgga ggcgtaaggc tgaaagacat
    49981 gcacccgaca gaacgtggta aagtcatcac ggctgcgggg ctcgttgttg ccgcgcgggt
    50041 tatggtcacc aagcgcggca atcgtatcgg tatctgcacg ctggatgacc gttccgggcg
    50101 gctggaagtg atgttgttta ctgacgccct ggataaatac cagcaattgc tggaaaaaga
    50161 ccgcatactt atcgtcagcg gacaggtcag ctttgatgac ttcagcggtg ggcttaaaat
    50221 gaccgctcgc gaagtgatgg atattgacga agcccgggaa aaatatgctc gcgggcttgc
    50281 tatctcgctg acggacaggc aaattgatga ccagctttta aaccgactcc gtcagtctct
    50341 ggaaccccac cgctctggga caattccagt acatctctac tatcagaggg cggatgcacg
    50401 cgcgcggttg cgttttggcg cgacgtggcg tgtctctccg agcgatcgtt tattaaacga
    50461 tctccgtggc ctcattggtt cggagcaggt ggaactggag tttgactaat acaggaatac
    50521 tatgagtctg aatttccttg attttgaaca gccgattgca gagctggaag cgaaaatcga
    50581 ttctctgact gcggttagcc gtcaggatga gaaactggat attaacatcg atgaagaagt
    50641 gcatcgtctg cgtgaaaaaa gcgtagaact gacacgtaaa atcttcgccg atctcggtgc
    50701 atggcagatt gcgcaactgg cacgccatcc acagcgtcct tataccctgg attacgttcg
    50761 cctggcattt gatgaatttg acgaactggc tggcgaccgc gcgtatgcag acgataaagc
    50821 tatcgtcggt ggtatcgccc gtcttgacgg tcgtccggtg atgatcattg gtcatcaaaa
    50881 aggtcgtgaa accaaagaaa aaattcgccg taactttggt atgccagcgc cagaaggtta
    50941 ccgcaaagca ctgcgtctga tgcaaatggc tgaacgcttt aagatgccaa tcatcacctt
    51001 tatcgacacc ccgggggctt atccgggcgt gggcgcagaa gagcgcggtc agtctgaagc
    51061 cattgcacgc aacctgcgtg aaatgtctcg ccttagcgta ccgactattt gtaccgttat
    51121 cggtgaaggt ggttctggcg gcgcgctggc gattggcgtg ggcgataaag tgaatatgct
    51181 gcaatacagc acctattccg ttatctcgcc ggaaggttgt gcgtccattc tgtggaagag
    51241 cgctgataaa gcgccgctgg cggctgaagc gatgggtatc atcgctccgc gtctgaaaga
    51301 actaaaactg atcgactcca tcatcccgga accgctgggt ggtgctcacc gtaacccgga
    51361 agcgatggcg gcatcgttga aagcgcaact gctggcggat ctggccgatc tcgatgtgtt
    51421 aagcactgaa gatttaaaaa atcgtcgtta tcagcgcctg atgagctacg gttacgcgta
    51481 attcgcaaaa gttctgaaaa agggtcactt cggtggccct tttttatcgc cacggtttga
    51541 gcaggctatg attaaggaag gattttccag gaggaacaca tgaacatcat tgccattatg
    51601 ggaccgcatg gcgtctttta taaagatgag cccatcaaag aactggagtc ggcgctggtg
    51661 gcgcaaggct ttcagattat ctggccacaa aacagcgttg atttgctgaa atttatcgaa
    51721 cataaccctc gaatttgcgg cgtgattttt gactgggatg agtacagtct cgatttatgc
    51781 agcgatatca atcaacttaa tgaatatctc ccgctttatg ccttcatcaa cacccactcg
    51841 acgatggatg tcagcgtgca ggatatgcgg atggcgctct ggttttttga atatgcgctg
    51901 gggcaggcgg aagatatcgc cattcgtatg cgtcagtaca ccgacgaata tcttgataac
    51961 attacaccgc cgttcacgaa agccttgttt acctacgtca aagagcggaa gtacaccttt
    52021 tgtacgccgg ggcatatggg cggcaccgca tatcaaaaaa gcccggttgg ctgcctgttt
    52081 tatgattttt tcggcgggaa taccctcaag gctgatgtct ctatttcggt caccgagctt
    52141 ggttcgttgc tcgaccacac cgggccacac ctggaagcgg aagagtacat cgcgcggact
    52201 tttggcgcgg aacagagtta tatcgttacc aacggaacat cgacgtcgaa caaaattgtg
    52261 ggtatgtacg ccgcgccatc cggcagtacg ctgttgatcg accgcaattg tcataaatcg
    52321 ctggcgcatc tgttgatgat gaacgatgta gtgccagtct ggctgaaacc gacgcgtaat
    52381 gcgttgggga ttcttggtgg gatcccgcgc cgtgaattta ctcgcgacag catcgaagag
    52441 aaagtcgctg ccaccacgca ggcacaatgg ccggttcatg cggtgattac caactccacc
    52501 tatgatggct tgctctacaa caccgactgg atcaaacaga cgctggatgt cccgtcgatc
    52561 cacttcgatt ccgcctgggt gccgtacacc cattttcatc cgatctacca ggggaaaagt
    52621 ggtatgagcg gcgagcgtgt tgcgggaaaa gtgatcttcg aaacgcaatc gacccacaaa
    52681 atgctggcgg cgttatcgca ggcttcgctg atccacatta aaggcgagta tgacgaagag
    52741 gcgtttaacg aagcctttat gatgcatacc accacctcgc ccagttatcc cattgttgct
    52801 tcggttgaga cggcggcggc gatgctgcgt ggtaatccgg gcaaacggct gattaaccgt
    52861 tcggtggagc gggcgctgca ttttcgcaaa gaggtccagc ggttgcggga agagtctgac
    52921 ggttggtttt tcgatatctg gcaaccgccg caggtggatg aagccgaatg ctggcccgtt
    52981 gcgccaggcg aacagtggca cggctttaac gatgcggatg ccgatcatat gtttctcgat
    53041 ccggttaaag tcactatttt gacaccgggg atggacgaac agggcaatat gagcgaggag
    53101 gggatcccgg cggcgctggt ggcaaaattc ctcgacgaac gtgggatcgt agtagagaaa
    53161 accggccctt ataacctgct gtttctcttt agtattggta tcgataaaac caaagcaatg
    53221 ggattattgc gtgggttgac ggaatttaaa cgctcttacg atctcaacct gcggatcaaa
    53281 aatatgctac ccgatctcta tgctgaagat cccgatttct accgcaatat gcgtattcag
    53341 gatctggcgc aagggatcca taagctgatt cgtaaacacg atcttcccgg tttgatgttg
    53401 cgggcattcg atactttgcc ggaaatgatc atgacgccac atcaggcatg gcagagacag
    53461 attaaaggtg aagtagaaac cattgcgctg gaacaactgg tcggtagagt atcggcaaat
    53521 atgatcctgc cttatccacc gggcgtaccg ctgttgatgc caggagaaat gctgaccaaa
    53581 gagagccgca cggtactcga ttttctactg atgctttgtt ccgtagtgca acattacccc
    53641 ggttttgaaa cggatattca cggcgcgaaa caggacgaag acggcgttta ccgcgtacga
    53701 gtcctaaaaa tggcgggata acttgccaga gcggcttccg ggcgagtaac gtgctgttaa
    53761 caaataaagg agacgttatg ctgggtttaa aacaggttca ccatattgcg attattgcga
    53821 cggattatgc ggtgagcaaa gctttctact gcgatattct tggtttcacg ctgcaaagcg
    53881 aagtctatcg cgaagcgcgc gactcgtgga aaggggattt ggcgcttaat gggcaatatg
    53941 tgattgagct tttctctttt ccgttcccgc cggagcgccc cagccgaccg gaagcttgcg
    54001 gcctgcgtca tctggctttt agcgttgatg acatcgatgc ggcagtggcg caccttgaaa
    54061 gccataacgt gaagtgtgaa gccatccgtg tcgatccata cacgcaaaaa cgcttcacct
    54121 tctttaacga tccggacggg ctgccgttgg aactgtatga gcagtaaggc ttgtcatcgc
    54181 cgcatttgcc cggtaacgtg ccgggcattg ctactgtaaa atcgcaccat catgacactc
    54241 acgctcaata gacaacttct cacctcacgc cagattctgg tggcctttag cggcgggctt
    54301 gactccaccg ttctgctgca tcagttggtg cagtggcgga cggaaaatcc gggcgtcact
    54361 ctgcgcgcta ttcatgtgca tcacggttta agtgccaatg ccgatgcctg ggttacgcat
    54421 tgcgaaaacg tctgccaaca gtggcaggtg ccgctggtgg tcgaacgcgt gcaacttgcg
    54481 caagaaggac tgggcattga ggcccaggcg cggcaggcgc gttatcaggc atttgcccgc
    54541 accttgttgc ccggtgaagt gctggtcacc gcgcaacatc tcgacgatca atgtgaaact
    54601 tttctgctgg cgctaaaacg cggtagcggc cctgccgggc tttcggctat ggcggaagtt
    54661 tcggagtttg ccggaacacg gcttatccgc ccgttgctcg cccgcacgcg gggagaactg
    54721 gagcagtggg cattggcgca tggtttacgc tggattgaag acgaaagtaa tcaggacgac
    54781 tcatacgatc gtaactttct gcgcctgcgc gtagtgccgt tattgcagca gcgttggccg
    54841 cattttgcgg aagcaacggc ccgcagcgcc gcactttgtg ctgaacaaga gagtctgttg
    54901 gatgaactgc tggcggatga tttagcacac tgccaaacgt cgcaggggac gctgcagatt
    54961 gcgccaatgc tggcgatgag tgatgcccgc cgcgcggcga ttatccgtcg ctggctggca
    55021 gggcagaatg caccaatgcc ttcccgcgac gcgctggtgc ggatctggca ggaagtggcg
    55081 ctggcgcggg aagatgcctc cccctgttta cgcttgggcg cgtttgaaat ccgccgctat
    55141 cagtcgcaac tgtggtggat taaatccgtc accgggcaaa gtgaaaccat tgtgccgtgg
    55201 cagacgtggc tacaaccgct ggaattaccg gcggggctgg gaagcgtaca gcttactgcg
    55261 ggaggcgata ttcgccctcc gcgtgcagac gaagcggtca gcgtgcgttt caaagcggca
    55321 ggattgctgc atattgtcgg gcgcaacggc ggacgtaagc tgaagaaaat ctggcaagag
    55381 ttaggcgtgc cgccgtggct acgtgacacc acgccactgc tgttttatgg cgaaacgctg
    55441 attgcggcgg caggggtatt tgtgacgcaa gaaggtgtgg ctgaaggtga gaatggcgta
    55501 agttttgtct ggcagaaaac gcttagttaa gtgaaagccg gataagacgc atcaaacgtc
    55561 gcatccggcg aaagttaatc aggactcgct caccaccacc gtaccgattt ccgggtggct
    55621 aaagctggta attttatcca gacgtagctc acgggtttcg ccagcggcct cgacgaccag
    55681 atactccaca tttttgcggg agactaaatc gctggctttt gcctgcaatt tttcgccatc
    55741 tttcagctca agtgtcagca ttaaatgatg ctggcaggcg agctcaagat tatcgtaatc
    55801 atcacaattg attggttgat acgtatcatt cattgacata atcgctcacc agtaagtttg
    55861 ccgcagcgta tgctgctttt tccctgacag cctcagaaag ggcgtcgtcg gcagccattt
    55921 cattcagcac tttcaaaacg cagcccagcg cgtccggaac gtatcctaag tctccgctgg
    55981 cgatttccgc gtaccgcttg cgtattaact cacaatattt ttccacatgc cctcctgtca
    56041 gcactctgac ttaaccgtgg atgcaagtct aagcctacga agataaactc tgtttcgcaa
    56101 ggtgactata ccacactcat ttctgcaata tcagcgccgc aactgcacgt attccgttac
    56161 aatggcctcc tgattcgaaa ggagttttct tatggcgctt aaagcgacaa tttataaagc
    56221 gacggttaat gtggccgatc tcgaccgcaa ccagtttctc gatgcctctc tgacgctggc
    56281 gcgccaccct tcagaaaccc aggagcgtat gatgctgcgc ttgctggcgt ggctgaaata
    56341 tgctgatgaa cgtctgcaat ttacccgtgg tttgtgtgcc gatgatgagc cggaagcgtg
    56401 gctgcgtaac gatcacctgg gcattgattt gtggattgag ctggggctgc cggatgagcg
    56461 gcggattaag aaagcctgca cccaggccgc agaagtggcg ctgtttacct ataatagtcg
    56521 ggcggcgcaa atctggtggc agcaaaatca gagcaaatgt gtgcagtttg ccaatctttc
    56581 cgtctggtat ctggacgatg aacaactggc gaaagtaagc gtctttgccg atcgtaccat
    56641 gacgctgcag gcaacgattc aggatggcgt gatctggtta tcggacgata agaataatct
    56701 ggaagttaac ttaaccgcct ggcaacaacc ttcatgattg tgatttcccg acatgttgct
    56761 attcccgatg gtgagcttga gatcaccgcc attcgtgcgc agggcgcggg cgggcagcat
    56821 gttaataaga cctcaacggc cattcatctg cgttttgaca ttcgggcgtc cagcctgcca
    56881 gagtattaca aagagcgtct actcgccgcc agccatcatt tgatcagcag tgatggcgtg
    56941 attgtcatta aggcacagga ataccgcagt caggaactga accgcgaagc cgcgctagcc
    57001 cggctggtgg cagtgattaa agatttaaca acagaacaaa aagcccgacg acccacgcgg
    57061 cccacccgtg catcgaaaga gcgcaggctg gcatcgaaag cacaaaaatc aagcgtgaag
    57121 gcgatgcgcg gcaaagtacg cagcggtcgg gaataaaaag aaggaataga tggtgaaaaa
    57181 agcgatagtg acagcgatgg ctgtaatcag cctctttact ctgatgggat gtaataaccg
    57241 ggccgaagtc gatacgcttt ctccagcaca ggctgccgaa ctgaaaccga tgccgcaaag
    57301 ttggcgcggc gtgctgccgt gtgctgattg cgaaggaatc gaaacctctc tgttcctcga
    57361 aaaagacgga acatgggtga tgaatgagcg ttatctcggt gctcgtgaag aaccttcctc
    57421 cttcgcttcc tacggtacat gggcgcgaac cgctgacaag ctggtattaa ccgacagcaa
    57481 aggtgaaaag tcatattatc gggcgaaagg cgatgcgctg gagatgctcg atcgtgaagg
    57541 caatccgatt gaatcgcagt tcaactatac gctggaaccg gcacaatcta gtttacctat
    57601 gacgccgatg accctgcgag gcatgtattt ttatatggct gatgcggcga ccttcactga
    57661 ttgcgcgacc ggaaaacgtt tcatggtagc gaataacgca gagctggagc gtagctacct
    57721 ggctgcgcgc ggtcacagtg aaaaaccggt gttactgtca gtagaaggtc actttacgct
    57781 tgaggctaat ccggatactg gtgcgccaac caaagtatta gcgcccgata cggcgggtaa
    57841 attttacccg aataaggatt gcagtagttt ggggcagtaa acctgcttaa ggcagaaaca
    57901 aacgcaaaat tgcctgatgc gctgcgctta tcaggcctac agcatataat gcaacatttt
    57961 gaattagcac gattttgtag gccggataag gcgtttacgc cgcatccggc attttgtgca
    58021 cgaggctggc tttatcactg agtcagccca acaaagcgac ccgccttgat ataaatccca
    58081 ggcttcaggt gcccaacata ctgtaactgc gtttctggct taaacgcaga gacatcacca
    58141 atacgcatat gcatcagatc ggcagggcta atccagcccg attgcgccag tgtaagtgga
    58201 tgcccggctt tggcaaaggc atccgtgaca aattccgaac aaaaccacga ctttttgtct
    58261 ccttcgccca cactgcttaa ctgcgctttc gccaggccgc tgacgcactt tggcgaaaat
    58321 cctcggagaa cggattcagt gagcacatct ggcgagtcac cataaaggga ataaattcga
    58381 caatgccgcg atagttataa ccgctatctt tgattttatt ggcaaacgcg gtgatatctg
    58441 tggcttgttg cggggtaaga tccgggactc gtaagacgaa aagcttatca ctatgcttca
    58501 tcgctttttt aagggaaaca atctggacgc cagcgcctgt cgcttctgca acgttattat
    58561 cacccagaaa gattgcaacg tgactcacag aggaagtgct gaagacgcgg attccaaatg
    58621 aggttacccc aaggcttgag gagaacagca aatcgccggg tttgagatca ggtgccgtta
    58681 tttcttttat tgattgttcg gtgaaagagc tttgatgctg gaatttgaca gcccatgttt
    58741 ttgcctcggc atctactgct gtggctgatg ggtctggctg gctgatatca acggtacaag
    58801 ccgaaagtaa aagaaagctg gggagaaaca ggcggcagta cgcctttggt ttatccattt
    58861 tatacaatcc atgtaaaaaa agggccctga aattcaggac cctttctggc atcagccttt
    58921 aatctgtttc accagatatt cgacgatgtc accagtctta attaactgtt tctcgccgtt
    58981 gcgacgatat ttatattcga tatcgtcgtt gtcgaggtta cggtcgccca gcacaatagt
    59041 gtgcggaata ccgatcagtt ccatatcagc aaacatcacg cccggacgct ctttgcggtc
    59101 atccagcagc acttcgatac cttgtgcacg cagttcgctg tacagtttct cagcaagctc
    59161 ttgtacgcgg aaggatttgt gcatgttcat cggcagaatc gccacctgga acggcgcgat
    59221 agcgtcaggc catacgatgc ctcgttcgtc gtagttctgc tcaatcgccg cagctaccac
    59281 acgcgttacc ccgataccgt agcaacccat cgtcaggatt tggttacggc catcttcacc
    59341 ctgtacggag gctttcagtg cttcggagta cttggtaccc agctggaaga tgtgaccaac
    59401 ttcgatacca cgtttgatca gcagcgtacc ctggccatcc gggcttggat cgccagccac
    59461 cacgttacgg atatctgcaa cttccggggt agcgacatcg cgatcccagt tgatgccgaa
    59521 gtagtgttta ccatcgatgt tagcaccagc agcgaaatca ctcatcgccg caacggtacg
    59581 gtcaatcacc accggaatcg gcatgtttac cggacccagt gaacccggac cggctttaac
    59641 cacggcacga atttcttctt cggtcgcgaa agtcagcggg cttgcaacct gcggcagttt
    59701 ttctgcttta acttcgttca gctcgtgatc accgcgcacc agcagcgcaa ccagcgggaa
    59761 gctactgcct tcaaccgctt taaccagcag agtcttaacc gttttctcaa tcggcagatt
    59821 gaactgttca accagttccg cgatggtttt cgcgttcggc gtatcaacca gcgtcatttc
    59881 ctgggtagca gcagcgcgcg gttctttcgg cgcgatagct tctgccagtt caatgttcgc
    59941 tgcatagtca gaggtgtcgg agaagaccac atcgtcttca ccgctctgcg ccagcacctg
    60001 gaattcgtga gaggcgctgc cgccgataga accggtgtcg gcttgtacgg cgcggaaatc
    60061 cagccccatg cggctgaaga ttttgctgta ggccgcatac attgcatcgt aggtttcctg
    60121 cagggattcc tgagaagtat ggaaagagta agcatctttc atcaggaatt cgcgggaacg
    60181 catgacgccg aaacgcggac gcacttcgtc gcggaacttg gtctggatct gatagaagtt
    60241 cagcggcagc tgtttgtaag agctaagctc gttacgaatc aggtcagtga taacttcttc
    60301 atgagttggg ccgagtacga acggacgctc gccacggtca acaaaacgca gcagttccgg
    60361 accgtactgt tcccaacgac cactctcttg ccacaaatca gctggctgaa ccaccggcat
    60421 cgacacctcg atcgcaccgg cgttgttcat ctcttcacgc acgatgtttt cgactttttt
    60481 cagaacgcgc acgccggtcg gcagccaggt atataacccg gaggccagct tgcggatcat
    60541 cccggcgcgc agcatcagct gatggctgat cacctcggcg tcggcaggtg tctccttgag
    60601 agtggagagc aggtattggc tagtacgcat gttgttacgg ttccagttgg aaggtagaac
    60661 aggctcaagg cgagcctggg acaaaaaaaa gtgatttagt ttaccagtgc aaaagaaatg
    60721 tcaaaagaga agggcgtgaa tttaacgcgg ttccagcgca aagacttcaa aacctgcgtc
    60781 ggtgacgcgc cagcgaacgt taaaatcatg tagccagacg gcataggttt tgcccgtttc
    60841 ctcaccttta cgataggccg ggcgtgggtc ctgcgccagt acttcgcgga taaacagcgt
    60901 taactgcgga taacgcttct ccagcgtcaa aagctgcttt tcgacctctg cggtaaaact
    60961 caccgccatc tctgccgctg gcgcactttg cgcatagctg gcactggcat cgggaagcga
    61021 ttcggcaaag gggagatacg gtttgatatc cactaccggc gtaccatcga ccagatccag
    61081 actgccgagc ttcagaatca cgctgtcttt atggcaaaca acctctttca gctctaccag
    61141 cgacatacca attgggttag ggcggaaagt agagcgtgtt gcgaaaaccc ccattctggc
    61201 gttaccgccg aggcgcggtg gacgcacagt cggacgccag ccgccttcca tcgtttgatg
    61261 aaagacgaaa aggatccata aatggctgaa cgcttccagg ccgcgaacgg cgtcggcctg
    61321 gttgtaggga gcaatgagat gcagttcgcc gttggcgctt tttaccagac ctggctggcg
    61381 cggaacggcg aatttttctt tatagggcga gcgaataacg cctatttgct cgaactggaa
    61441 actgctcatt tcgccgtaat gttaagcgca gagccgatac atacagcctg acgatagcag
    61501 cctggcgtac cgctggtgac ttcgcagcta tgcagtaata ccgcattggc cttcattttc
    61561 gaggcgttga tttgcatccg cttacgcgcg gttggaatgc tcggcggaga gtcctgatta
    61621 gaggcctggc aagagtcgcc actgacttca ccgagatcgc ggaacggttt gccgactaat
    61681 tcttctgcat tggtataaat tcggaccggc gtggcgcgcg gcgctttcgg ttttgcaggc
    61741 tccgctttcg gctggggtgc agtgctttga acgggttcga caggggatct gcttaacatg
    61801 gaacagccgc ttagcatgag tgctactaaa cagatcggta aagcacgcat agtatttcct
    61861 caatgtatga tcaaaacgtc aatattgaat caggagcttg taaaaatgac aagacgggca
    61921 agcgcccgtc ctgaatgata ttacaaattg tggaaacagc ctaaaaatta ccagccttta
    61981 acagcgccgc cgttaaacac tttgtttgct gcttcgtaaa cttcatcaga ctgataagcc
    62041 tggacgaatt tcttcacgtt ctcggcgtct ttgttatctt cacgcgttac gatcaggttt
    62101 acgtacgggg agtctttatc ttcgacaaag ataccgtctt tagctggagt caggccaatc
    62161 tggctggcat aggtggtatt gataactgcc agagcgattt gcgcgtcgtc cagagatcga
    62221 ggcagctgcg gtgcttccag ctcaacaatt ttcagatttt ttgggttctc aacaacatca
    62281 agaacggtcg gcagcaggcc aacgccatct ttcagtttga tcaagcccac tttttgcagc
    62341 agcagcagtg aacgaccaag attagttgga tcgtttggca cggcaacctg cgaaccatcc
    62401 tgcagttcat ccagtgattt gattttcttg gagtaaccag caatcggata aacaaaagtg
    62461 ttgcctactg cgaccagttt gtagccacga tctttcagtt gctgatcaag gtacggttta
    62521 tgctggaagg cgttggcgtc gatatcgcct ttgctcaatg cttcgtttgg cagaacatag
    62581 tcgttgaagg ttaccagctc aacgtccagg ccatatttgt ctttcgcaac tttctgcgcg
    62641 acttctgcta cctgctgttc ggcaccaaca atcacgccga ctttaatgtg gtttggatct
    62701 ttttcatcct gaccgcagcc taccagtgcc agtgatccaa tcagggctcc cactgccgca
    62761 aaggttttga atttgaacgc cataccttat tccttcttca attatttatg ttgtgttgaa
    62821 cgttacttgc gagtgacagc ccggacgatg cggtcgcctg cgaactgaat taaataaacc
    62881 agaatgacca gcaataccag taccgtattc atcaccgtcg cgttatagcc gatgtagcca
    62941 tactgatagc caatctgacc taaaccaccg gcaccgactg caccacccat cgcggaataa
    63001 ccgaccaggg taatcagggt gatagttgcc gcattcacca gacccggcaa tgcttccggt
    63061 aacagcactt tacggacgat ctgcatcggc gtggcaccca ttgcgcggga agcttcaatt
    63121 aacccggttg ggatctccag cagagcgttc tcgaccatac gggcaataaa cggtgctgca
    63181 ccaacggtta acggaacaat cgctgcctgc aaaccaatcg atgtaccaac aataacgcgg
    63241 gtaaacggaa tcatccatac cagcaagata atgaacggga tagaacggaa aatgttcaca
    63301 atcgcagaaa cggtacgata cagcttcgcg ttagcaataa tttgccccgg acgcgtgaca
    63361 taaagcagaa cgccaaccgg cagaccaatc acaaagccaa aaaaaccgga tacgaaggtc
    63421 attgccagcg tttcccatac gccacgaacc agcagccaca tcatcggctc agacataacc
    63481 cagtacctct acttttacat ggtgttcctg cagccaggca atggcggctt gcgtatcttg
    63541 ttgtgtgccg tgcatttcag tcagcatgat gccgaactta acgccaccgg cgtagtccat
    63601 ctgcgcgcta ataatgttgt tgttgacgtt gaaacgacgc gcggtttcag aaagcagtgg
    63661 ggcatcgacc gactgaccgg taaactccag acgcagcatc ggcacgcagt cagtgaatgg
    63721 ctccgcttgc agacgttcct ggtaatcttc cgggatatcc agatgcaggg tcgactgaat
    63781 aaacttctgc gccaacggcg ttttcggatg cgaaaacact tcacttaccg tgtcctgctc
    63841 gatcagttcg ccattgctga tgaccgccac gcaatcacaa atacgcttta caacgtccat
    63901 ttcgtgggtg atcaacagaa tcgtcaaacc cagacggcgg ttaatgtctt tcagcagctc
    63961 gagaatagaa cgtgtcgttg ccgggtccag cgcgctggtg gcttcatcac acagcaatac
    64021 tttgggattg ctggctaacg cacgggcaat cgccacacgc tgtttctgcc caccggaaag
    64081 attcgatggg tagctatcat gcttatcgcc aagaccaacc aatgacagca attccgtcac
    64141 gcgacgtttg atctcgtctt tcggtgtgtt gtccagctcc agcggcagag ccacgttgcc
    64201 aaaaacagta cgcgaagaga gcaggttaaa atgctggaaa atcataccaa tctggcggcg
    64261 agctttggtc aactcggatt ctgacagcgt ggtcagttcc tggccatcga ccagcacgct
    64321 accctcggtt gggcgctcca gcaggtttac acaacgtata agcgtactct tacccgcgcc
    64381 tgaggcaccg ataacgccat aaatttgtcc agctggcaca tgcaggctga cgttgttcaa
    64441 cgcctggatg gtgcgggtgc cctggtggaa cactttggtg atattcgaaa gttttatcat
    64501 tgattattta ttatcgtcat taagttagtc gtggcatctc gaatgcctga aacgggcaac
    64561 gccgtcaatg aaatggatgt taaggcatcc agacgtctaa atcaatcagg tttatgcgaa
    64621 gagcactttc ttgcaggtcg aaacatgcga tactagcgtc acatgcctta ttaaggagct
    64681 ataaaaggtg gcaaagagcg tacccgcaat ttttcttgac cgtgatggca ccattaatgt
    64741 cgatcacggc tatgtccatg agatcgacaa ctttgaattt atcgacggtg ttattgacgc
    64801 catgcgcgag ctaaaaaaaa tgggctttgc gctggtggta gtaaccaacc agtctggcat
    64861 tgctcgcggt aaatttaccg aagcacagtt tgaaacgctg accgagtgga tggactggtc
    64921 gctggcggac cgagatgtcg atctggatgg tatctattat tgcccgcatc atccgcaggg
    64981 tagtgttgaa gagtttcgcc aggtctgcga ctgccgtaaa ccacatccgg ggatgctttt
    65041 gtcagcacgc gattatttgc atattgatat ggccgcttct tatatggtgg gcgataaatt
    65101 agaagatatg caggcagcgg ctgcggcgaa cgtgggaaca aaagtgctgg tgcgtacggg
    65161 taaacctatt acgcctgaag cagaaaacgc ggcggattgg gtgttaaata gcctggcaga
    65221 cctgccgcaa gcgataaaaa agcagcaaaa accggcataa tgattaaaag atgagcaatt
    65281 gaaataaaaa tgcatttttc cacttgtctt cctgagacga ctccctataa tgcgcctcca
    65341 tcgacacggc ggatgtgaat cacttcacac aaacagccgg ttcggttgaa gagaaaaatc
    65401 ctgaaattca gggttgactc tgaaagagga aagcgtaata tacgccacct cgcgacagtg
    65461 cgctaaagcg cgtcgcaact gctctttaac aatttatcag gcaatctgtg tgggcactcg
    65521 aagatacgga ttcttaacgt cgcaagacga aaaatgaata ccaagtctca agagtgaaca
    65581 cgtaattcat tacgaagttt aattctttga gcatcaagct tttaaattga agagtttgat
    65641 catggctcag attgaacgct ggcggcaggc ctaacacatg caagtcgaac ggtaacagga
    65701 agcagcttgc tg
//
LOCUS       DAZFXT010000012        64404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.012, whole genome shotgun sequence.
ACCESSION   DAZFXT010000012 DAZFXT010000000
VERSION     DAZFXT010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 64404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.012"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1344)
                     /locus_tag="ACHKW0_000850"
                     /pseudo
     CDS             complement(1..1344)
                     /locus_tag="ACHKW0_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418674.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(1467..1919)
                     /gene="tabA"
                     /locus_tag="ACHKW0_000851"
     CDS             complement(1467..1919)
                     /gene="tabA"
                     /locus_tag="ACHKW0_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HFO7473732.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            complement(2064..2657)
                     /gene="bdcR"
                     /locus_tag="ACHKW0_000852"
     CDS             complement(2064..2657)
                     /gene="bdcR"
                     /locus_tag="ACHKW0_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7473733.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAISAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPLARDIAVQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            2728..3441
                     /gene="bdcA"
                     /locus_tag="ACHKW0_000853"
     CDS             2728..3441
                     /gene="bdcA"
                     /locus_tag="ACHKW0_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7473734.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     KRLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGEALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSLMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            3572..3967
                     /gene="yjgH"
                     /locus_tag="ACHKW0_000854"
     CDS             3572..3967
                     /gene="yjgH"
                     /locus_tag="ACHKW0_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HFO7473735.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            4248..4382
                     /gene="pyrL"
                     /locus_tag="ACHKW0_000855"
     CDS             4248..4382
                     /gene="pyrL"
                     /locus_tag="ACHKW0_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HFO7473736.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            4386..5321
                     /gene="pyrB"
                     /locus_tag="ACHKW0_000856"
     CDS             4386..5321
                     /gene="pyrB"
                     /locus_tag="ACHKW0_000856"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HFO7473737.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            5334..5795
                     /gene="pyrI"
                     /locus_tag="ACHKW0_000857"
     CDS             5334..5795
                     /gene="pyrI"
                     /locus_tag="ACHKW0_000857"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009347 - aspartate carbamoyltransferase
                     complex [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HFO7473738.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            5868..6254
                     /gene="ridA"
                     /locus_tag="ACHKW0_000858"
     CDS             5868..6254
                     /gene="ridA"
                     /locus_tag="ACHKW0_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HFO7473739.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            6320..6775
                     /locus_tag="ACHKW0_000859"
     CDS             6320..6775
                     /locus_tag="ACHKW0_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7473740.1"
                     /translation="MAKPRFTNEQIAEILQQSKEGASNKELCEHYQFSVSTLRRWQEQ
                     HADGIRSELKKTESKAQIVFLVFFAIAILLTLIFDKPTGGWVIPPLLIYCVYYIRQYR
                     NISGRHIKKEDIYLSRSVNNSYSALYNLSWTFICFFIFAVIYFFIQVFS"
     gene            complement(6820..9516)
                     /gene="mgtA"
                     /locus_tag="ACHKW0_000860"
     CDS             complement(6820..9516)
                     /gene="mgtA"
                     /locus_tag="ACHKW0_000860"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HFO7473741.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(9657..9710)
                     /gene="mgtL"
                     /locus_tag="ACHKW0_000861"
     CDS             complement(9657..9710)
                     /gene="mgtL"
                     /locus_tag="ACHKW0_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HFO7473742.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            9895..10842
                     /gene="treR"
                     /locus_tag="ACHKW0_000862"
     CDS             9895..10842
                     /gene="treR"
                     /locus_tag="ACHKW0_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor TreR"
                     /protein_id="HFO7473743.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLNPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            10961..12382
                     /gene="treB"
                     /locus_tag="ACHKW0_000863"
     CDS             10961..12382
                     /gene="treB"
                     /locus_tag="ACHKW0_000863"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015574 - trehalose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0015771 - trehalose transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HFO7473744.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMVIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            12432..14087
                     /gene="treC"
                     /locus_tag="ACHKW0_000864"
     CDS             12432..14087
                     /gene="treC"
                     /locus_tag="ACHKW0_000864"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008788 - alpha,alpha-phosphotrehalase
                     activity [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HFO7473745.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYISPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIISRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKLEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMTLRPFEAVWWLQK"
     gene            14481..16619
                     /gene="nrdD"
                     /locus_tag="ACHKW0_000865"
     CDS             14481..16619
                     /gene="nrdD"
                     /locus_tag="ACHKW0_000865"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HFO7473746.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            16777..17241
                     /gene="nrdG"
                     /locus_tag="ACHKW0_000866"
     CDS             16777..17241
                     /gene="nrdG"
                     /locus_tag="ACHKW0_000866"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HFO7473747.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QLFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(17286..17672)
                     /gene="cybC"
                     /locus_tag="ACHKW0_000867"
     CDS             complement(17286..17672)
                     /gene="cybC"
                     /locus_tag="ACHKW0_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HFO7473748.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(17855..19207)
                     /gene="pmbA"
                     /locus_tag="ACHKW0_000868"
     CDS             complement(17855..19207)
                     /gene="pmbA"
                     /locus_tag="ACHKW0_000868"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HFO7473749.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDDAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            19301..19852
                     /gene="yjgA"
                     /locus_tag="ACHKW0_000869"
     CDS             19301..19852
                     /gene="yjgA"
                     /locus_tag="ACHKW0_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HFO7473750.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(20008..21381)
                     /gene="mpl"
                     /locus_tag="ACHKW0_000870"
     CDS             complement(20008..21381)
                     /gene="mpl"
                     /locus_tag="ACHKW0_000870"
                     /EC_number="6.3.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HFO7473751.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            21557..22555
                     /gene="fbp"
                     /locus_tag="ACHKW0_000871"
     CDS             21557..22555
                     /gene="fbp"
                     /locus_tag="ACHKW0_000871"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HFO7473752.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(22588..23583)
                     /gene="yjfF"
                     /locus_tag="ACHKW0_000872"
     CDS             complement(22588..23583)
                     /gene="yjfF"
                     /locus_tag="ACHKW0_000872"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HFO7473753.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(23570..24592)
                     /gene="ytfT"
                     /locus_tag="ACHKW0_000873"
     CDS             complement(23570..24592)
                     /gene="ytfT"
                     /locus_tag="ACHKW0_000873"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfT"
                     /protein_id="HFO7473754.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMSQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITSGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(24606..26108)
                     /gene="ytfR"
                     /locus_tag="ACHKW0_000874"
     CDS             complement(24606..26108)
                     /gene="ytfR"
                     /locus_tag="ACHKW0_000874"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, ATP-binding
                     protein YtfR"
                     /protein_id="HFO7473755.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            complement(26248..27204)
                     /gene="ytfQ"
                     /locus_tag="ACHKW0_000875"
     CDS             complement(26248..27204)
                     /gene="ytfQ"
                     /locus_tag="ACHKW0_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HFO7473756.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            27514..28044
                     /gene="ppa"
                     /locus_tag="ACHKW0_000876"
     CDS             27514..28044
                     /gene="ppa"
                     /locus_tag="ACHKW0_000876"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HFO7473757.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(28124..28474)
                     /gene="chpB"
                     /locus_tag="ACHKW0_000877"
     CDS             complement(28124..28474)
                     /gene="chpB"
                     /locus_tag="ACHKW0_000877"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HFO7473758.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(28468..28719)
                     /gene="chpS"
                     /locus_tag="ACHKW0_000878"
     CDS             complement(28468..28719)
                     /gene="chpS"
                     /locus_tag="ACHKW0_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HFO7473759.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            complement(28932..29273)
                     /gene="ytfP"
                     /locus_tag="ACHKW0_000879"
     CDS             complement(28932..29273)
                     /gene="ytfP"
                     /locus_tag="ACHKW0_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HFO7473760.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(29276..33055)
                     /gene="tamB"
                     /locus_tag="ACHKW0_000880"
     CDS             complement(29276..33055)
                     /gene="tamB"
                     /locus_tag="ACHKW0_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HFO7473761.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAAERWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     SQGIVNASGTAQLSDNWPVDITLNCTLNVEPLKGEKLKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGLEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEIPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVTQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPYATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(33052..34785)
                     /gene="tamA"
                     /locus_tag="ACHKW0_000881"
     CDS             complement(33052..34785)
                     /gene="tamA"
                     /locus_tag="ACHKW0_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HFO7473762.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            34991..35629
                     /gene="msrA"
                     /locus_tag="ACHKW0_000882"
     CDS             34991..35629
                     /gene="msrA"
                     /locus_tag="ACHKW0_000882"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HFO7473763.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            35952..37295
                     /gene="paeA"
                     /locus_tag="ACHKW0_000883"
     CDS             35952..37295
                     /gene="paeA"
                     /locus_tag="ACHKW0_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HFO7473764.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(37357..37563)
                     /gene="ytfK"
                     /locus_tag="ACHKW0_000884"
     CDS             complement(37357..37563)
                     /gene="ytfK"
                     /locus_tag="ACHKW0_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HFO7473765.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            37888..38445
                     /gene="ytfJ"
                     /locus_tag="ACHKW0_000885"
     CDS             37888..38445
                     /gene="ytfJ"
                     /locus_tag="ACHKW0_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7473766.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            complement(38435..39175)
                     /gene="cysQ"
                     /locus_tag="ACHKW0_000886"
     CDS             complement(38435..39175)
                     /gene="cysQ"
                     /locus_tag="ACHKW0_000886"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HFO7473767.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            39365..41308
                     /gene="cpdB"
                     /locus_tag="ACHKW0_000887"
     CDS             39365..41308
                     /gene="cpdB"
                     /locus_tag="ACHKW0_000887"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008663 - 2',3'-cyclic-nucleotide
                     2'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HFO7473768.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(41437..41817)
                     /gene="ytfH"
                     /locus_tag="ACHKW0_000888"
     CDS             complement(41437..41817)
                     /gene="ytfH"
                     /locus_tag="ACHKW0_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HFO7473769.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            41906..42766
                     /gene="qorB"
                     /locus_tag="ACHKW0_000889"
     CDS             41906..42766
                     /gene="qorB"
                     /locus_tag="ACHKW0_000889"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7473770.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADKSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDDSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            42875..43840
                     /gene="ytfF"
                     /locus_tag="ACHKW0_000890"
     CDS             42875..43840
                     /gene="ytfF"
                     /locus_tag="ACHKW0_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7473771.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNTQ
                     TPDFSLPFGPRPLVFISLMIAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            43948..44610
                     /gene="ytfE"
                     /locus_tag="ACHKW0_000891"
     CDS             43948..44610
                     /gene="ytfE"
                     /locus_tag="ACHKW0_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HFO7473772.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(44655..46067)
                     /gene="cycA"
                     /locus_tag="ACHKW0_000892"
                     /gene_synonym="dagA"
     CDS             complement(44655..46067)
                     /gene="cycA"
                     /locus_tag="ACHKW0_000892"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HFO7473773.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(46376..46996)
                     /gene="fklB"
                     /locus_tag="ACHKW0_000893"
     CDS             complement(46376..46996)
                     /gene="fklB"
                     /locus_tag="ACHKW0_000893"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7473774.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            47215..47853
                     /gene="ytfB"
                     /locus_tag="ACHKW0_000894"
     CDS             47215..47853
                     /gene="ytfB"
                     /locus_tag="ACHKW0_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HFO7473775.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(47837..48484)
                     /locus_tag="ACHKW0_000895"
     CDS             complement(47837..48484)
                     /locus_tag="ACHKW0_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HFO7473776.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            48697..49488
                     /locus_tag="ACHKW0_000896"
     CDS             48697..49488
                     /locus_tag="ACHKW0_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HFO7473777.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            49498..50340
                     /locus_tag="ACHKW0_000897"
     CDS             49498..50340
                     /locus_tag="ACHKW0_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HFO7473778.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            50350..51126
                     /locus_tag="ACHKW0_000898"
     CDS             50350..51126
                     /locus_tag="ACHKW0_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HFO7473779.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNLPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            51136..52677
                     /locus_tag="ACHKW0_000899"
     CDS             51136..52677
                     /locus_tag="ACHKW0_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7473780.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRDLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDDLVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            52674..54755
                     /locus_tag="ACHKW0_000900"
     CDS             52674..54755
                     /locus_tag="ACHKW0_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HFO7473781.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNIDGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            54776..55252
                     /locus_tag="ACHKW0_000901"
     CDS             54776..55252
                     /locus_tag="ACHKW0_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO7473782.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            55295..56596
                     /locus_tag="ACHKW0_000902"
     CDS             55295..56596
                     /locus_tag="ACHKW0_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO7473783.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            56649..57620
                     /locus_tag="ACHKW0_000903"
     CDS             56649..57620
                     /locus_tag="ACHKW0_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO7473784.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENFYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(57732..58181)
                     /gene="rplI"
                     /locus_tag="ACHKW0_000904"
     CDS             complement(57732..58181)
                     /gene="rplI"
                     /locus_tag="ACHKW0_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HFO7473785.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(58223..58450)
                     /gene="rpsR"
                     /locus_tag="ACHKW0_000905"
     CDS             complement(58223..58450)
                     /gene="rpsR"
                     /locus_tag="ACHKW0_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HFO7473786.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            complement(58455..58769)
                     /gene="priB"
                     /locus_tag="ACHKW0_000906"
     CDS             complement(58455..58769)
                     /gene="priB"
                     /locus_tag="ACHKW0_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030894 - replisome [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HFO7473787.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            complement(58776..59171)
                     /gene="rpsF"
                     /locus_tag="ACHKW0_000907"
     CDS             complement(58776..59171)
                     /gene="rpsF"
                     /locus_tag="ACHKW0_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HFO7473788.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            59498..59773
                     /gene="yjfY"
                     /locus_tag="ACHKW0_000908"
     CDS             59498..59773
                     /gene="yjfY"
                     /locus_tag="ACHKW0_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YjfY"
                     /protein_id="HFO7473789.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDMQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            complement(59902..60588)
                     /gene="ulaF"
                     /locus_tag="ACHKW0_000909"
     CDS             complement(59902..60588)
                     /gene="ulaF"
                     /locus_tag="ACHKW0_000909"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HFO7473790.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(60588..61442)
                     /gene="ulaE"
                     /locus_tag="ACHKW0_000910"
     CDS             complement(60588..61442)
                     /gene="ulaE"
                     /locus_tag="ACHKW0_000910"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HFO7473791.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            complement(61452..62102)
                     /gene="ulaD"
                     /locus_tag="ACHKW0_000911"
     CDS             complement(61452..62102)
                     /gene="ulaD"
                     /locus_tag="ACHKW0_000911"
                     /EC_number="4.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7473792.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            complement(62116..62580)
                     /gene="ulaC"
                     /locus_tag="ACHKW0_000912"
     CDS             complement(62116..62580)
                     /gene="ulaC"
                     /locus_tag="ACHKW0_000912"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HFO7473793.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            complement(62590..62895)
                     /gene="ulaB"
                     /locus_tag="ACHKW0_000913"
     CDS             complement(62590..62895)
                     /gene="ulaB"
                     /locus_tag="ACHKW0_000913"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HFO7473794.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSKIDHTVNSCAVGEY
                     KSELSNADIIIASTHIAGEISVTGNKYVVGVRNMLSPADFGPKLLEVIKEHFPQDVK"
     gene            complement(62911..64308)
                     /gene="ulaA"
                     /locus_tag="ACHKW0_000914"
     CDS             complement(62911..64308)
                     /gene="ulaA"
                     /locus_tag="ACHKW0_000914"
                     /EC_number="2.7.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7473795.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYN
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNIMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
BASE COUNT        15822 a        16905 c        16043 g        15634 t
ORIGIN      
        1 tcaggttgct tcatgaaaaa atccttcaaa aaaaatatca tttactaaaa tgctgacagg
       61 cacttcatta aatagcaact taacatcatc aaataacctt agtattaagc gtgatttttc
      121 ttgattgctt aagttaaaat cattgacata tgcaaccaac ttatcccaaa tcaattcctg
      181 taatttacgg ctatttttat agtgttcttt atcgagattg aaaaataagg tctcagctat
      241 tttaatttta gattgatcgc agctaaaatc tttagattga tcgcggctaa aatcaatatg
      301 atttatcacg gagcatgcat actttatctt ttcagaatca ccaaaatatt gcattctttt
      361 attaaaacac caatccagta ttcttacatc attaatatat gggaatttta aaaaaatctc
      421 cagaatcgaa tcgatgaccg gtgtaaggtt aacttttgca tcatctaagg aatcaataag
      481 aatatgagac aggttaactt tgatgtcatt aatttcattg tcagtagctt tcttattatt
      541 aagaagataa ttatttactg cgttaaggtc atctatttct ttcagtatcc ctttttcacc
      601 aagcaagtat gaaatcgatt cattacatac ataaggcatt aatagcagat gttctttcct
      661 tattgctaat aatgcattat tcaatggttt ttctaataaa tccgtaatcg tagaatcaac
      721 tgaatcttta caactcacct gcgagttatt caagtcctga gaattattta actcctgcgg
      781 aatatttaat agacgagcta tttccttgtt agcttcgcgt tggtaacacg ccagaatctt
      841 gttagtaatc aggtgtaatg aactctcatc ttttctttca aaggacttcc cttcataacg
      901 attagtcgaa ccatcaggtt gtgtaaatat aatgactgta tgtccttcac gaatcccatt
      961 attaaaagct gaggcaagtg taatttctat ttccacctta gataaatccg ctcgctcccc
     1021 ttcaggatta ttcataacaa acttcattat ctcactacca tcattatttt tgatataaga
     1081 tgaggactta atatgagcca tcatatttct acccgcttcg ttggagcgtt tgctaaaatc
     1141 cacataaccc agggtaaata tcttagccaa aaactccaaa aatgctctgg gagaaagaat
     1201 attgcttctg ctattctgcc ctccggcaat tcgggcaaaa tcattcattc ccacatacca
     1261 ggtatctaag tcttcatttt tttgtttaca attgtcggct gatgtaatgt tttgagaaaa
     1321 attccaacct gatattttgc tcatgacgga ctccttgagt taataagaac gtctaagcat
     1381 tacaaatttt actcacaaaa aaatatcaaa aaacgctctt ttcacaaact cgccgtaaat
     1441 ctggagttat ttacggcgaa aagtcatcac gccatcagca tcttcactac tgctttgcga
     1501 acctgggctg gtgcacccac tgcgcacagc ggtttatgca cttcccccgg ataaaacaca
     1561 acaaaatcac cttcattaag gataactgtt ttctcatcaa cgccttccgg caaaaatgcg
     1621 atgtctttat cagctaacca gtcggtatcc ggcgtgcctg caggttgcgt gctgaaggtc
     1681 atgccttcct gaccttttaa cacaatctga atgtcgagat agcgggcatg gtactccgca
     1741 cggcgggctt cgtacggctc ggtcatatct tccgagataa gataaaacag acgattgcct
     1801 tcgatatcgt gcttgccctt tggcgtttct gccgtaacgt gtgctttgat atgctcaatc
     1861 gcctggcgta actcctgggg tagccacggc tgaagattat gaatatttcc gatgatcatc
     1921 ttttccactc cttaattaaa acaacgtttc atttctatcg ttatacgaaa agatgatcaa
     1981 ttccgctagc cttaacgcgt gaattattgt cttatcacaa cttatcggca ggcgcgttcc
     2041 cgcctttcgg atcaaggcca gcatcactcc ttgagaagag tttttatcgc ttcccccgcc
     2101 agtgcagctg ttgcacagag ttgttcgatt gagtgcccct cccgtgcctt cgcagaaagc
     2161 cctgacatca cggtactcat aaaatcagtc acacattgtg cgctttgtgg atgccgcctg
     2221 gcgatatagt cataaatggt cgtttctgcg gcgtgataat attgaacggc aatatcacgc
     2281 gctagtggat catgactatg aataccttca agaaccatac agccagcgca gccgccgttt
     2341 tggctatatc tgcgcgccgc ttcttttaat acctcaacca ggcactcgcc tactggacga
     2401 tcatcacgaa gaatatcggc aagcggaata gcttccgtac cgacgtattc attgagtaca
     2461 cggctaaata acccagcttt actgccaaaa gccgcgtaga ggctcggggg gttaatacca
     2521 agataatcag taacttcagc aacactgaca gcatcgaaac ctttttggtg aaaaagcact
     2581 tttgccgcag agattgcctg ctcaggagcg aaacgtctgg gacgacctgg aacgcgagat
     2641 tgttttttag tgaccattac aaaacctgtt gacagaaagt taaaacagtt ttgtaatgca
     2701 tgttacataa taaatcaagg agtccttatg ggcgctttta caggtaagac agttctcatc
     2761 ctcggtggca gtcgtggtat cggtgccgct atcgtacgtc gtttcgtcac cgatggggcc
     2821 aatgtacgat tcacctatgc ggggtcgaaa gatgccgcta aacgcctggc acaagagact
     2881 ggagcgacag cagtattcac agatagtgct gacagagacg ctgtcattga tgtcgttcgt
     2941 aagagcggcg cattggatat cctggtggta aatgcaggta ttggcgtctt tggcgaggcc
     3001 ctggaattaa atgccgacga tattgatcgc cttttcaaaa tcaatattca tgctccttat
     3061 catgcctctg ttgaagccgc ccggcagatg cccgaaggcg ggcgcatctt aatcatcggc
     3121 tccgtgaatg gcgatcgtat gcctgttgca ggcatggctg cttatgccgc cagcaaatct
     3181 gccctgcaag gcatggcgcg cgggctggcc cgtgattttg gaccgcgtgg gatcaccatt
     3241 aacgtcgtcc agccagggcc aattgatacc gacgctaatc ccgccaacgg gccaatgcgc
     3301 gatatgttgc atagtttgat ggctatcaaa agacatgggc aaccggaaga ggtcgctggt
     3361 atggtcgcat ggttagcagg gccagaagcc agttttgtta ccggcgcgat gcataccatt
     3421 gatggcgcgt ttggcgcata accgactacg ctcaattaag cccagccatt atccatgatg
     3481 tctgggtttt gtttactcac gtcgtccgct aaaagcggct cccggtaaag agaaatcttc
     3541 tgttcatgtc gtaaatcttg aggataacca aatggtagaa agaaccgctg ttttccctgc
     3601 tggccgacat tcactatatg ctgagcatcg ttattctgcg gctattcgtt ccggcgattt
     3661 gctgtttgtt tccgggcaag taggaagtcg agaggacgga acaccagaac ccgattttca
     3721 gcaacaagtc agactggcat ttgataattt gcatgcgacc ctggcagctg cgggatgcac
     3781 ttttgacgat atcattgatg ttacgagctt ccataccgat ccagaaaacc aatttgaaga
     3841 catcatgacg gtgaaaaatg aaatatttag cgccccacct tatccaaact ggacggcggt
     3901 gggtgttaca tggctggcag gctttgattt tgaaattaaa gtgatagcgc gcatccctga
     3961 gcagtaagca atagtgttag ccgttcgctt tcacactccg ccctataagt cggatgaatg
     4021 gaataaaatg catatctgat tgcgtgaaag tgaaaaagaa aaaagcaggg aatgtctgca
     4081 attattgata ccgaacgaca gttcccctgc agaatcacat caaataaaaa tgcatatacc
     4141 ttgactttta attcaaataa accgtttgcg ctgacaaaat attgcatcaa atgcttgcgc
     4201 cgcttctgac gatgagtata atgccggaca atttgccggg aggatgtatg gttcagtgtg
     4261 ttcgacattt tgtcttaccg cgtctgaaaa aagacgctgg cctgccgttt ttcttcccgt
     4321 tgatcaccca ttcccagccc ctcaatcgag gggctttttt ttgcccaggc gtcaggagat
     4381 aaaagatggc taatccgcta tatcagaaac atatcatttc cataaacgac cttagtcgcg
     4441 atgaccttaa tctggtgctg gcgacagcgg cgaaactgaa agcaaacccg caaccagagc
     4501 tgttgaagca caaagtcatt gccagctgtt tcttcgaagc ctctacccgt acccgcctct
     4561 ctttcgaaac atctatgcac cgcctgggtg ccagcgtggt gggcttctcc gacagcgcca
     4621 atacatcact gggtaaaaag ggcgaaacgc tggccgatac catttcggtt atcagcactt
     4681 acgtcgatgc gatagtgatg cgtcatccgc aggaaggtgc ggcgcgcctg gccaccgagt
     4741 tttcaggcaa tgtaccggta ctgaatgccg gtgatggctc caaccaacat ccgacgcaaa
     4801 ccttgctgga cttattcact attcaggaaa cccaggggcg tctggacaat ctccacgtcg
     4861 caatggttgg tgacctgaaa tatggccgca ccgttcactc cctgactcag gcgttagcga
     4921 agttcgacgg caaccgtttt tacttcatcg cgccggacgc gctggcaatg ccgcaataca
     4981 ttctggatat gctcgatgaa aaagggatcg catggagtct gcacagctct attgaagaag
     5041 tgatggcgga agtagacatc ctgtacatga cccgcgtgca aaaagagcgt ctggacccgt
     5101 ccgagtacgc caacgtgaaa gcgcagtttg ttcttcgcgc cagcgatctc cacaacgcca
     5161 aagccaatat gaaagtgctg catccgctgc cgcgtgttga tgagattgcg acggatgttg
     5221 ataaaacgcc acacgcctgg tacttccagc aggcaggcaa cgggattttc gctcgccagg
     5281 cgttactggc actggttctg aatcgcgatc tggtactgta aggggaaata gagatgacac
     5341 acgataataa attgcaggtt gaagctatta aacgcggcac ggtaattgac catatccccg
     5401 cccagatcgg ttttaagctg ttgagtctgt tcaagctgac cgaaacggat cagcgcatca
     5461 ccatcggtct gaacttgcct tctggcgaga tgggccgcaa agatctgatc aaaatcgaaa
     5521 ataccttttt gagtgaagat caggtagatc aactggcatt gtatgcgccg caagccacgg
     5581 ttaaccgtat cgacaactat gaagtggtgg gtaaatcgcg ccccagtctg ccagagcgca
     5641 tcgacaatgt gctggtctgc ccgaacagca actgtatcag ccatgccgaa ccggtttctt
     5701 ccagctttgc cgtgcgaaaa cgcgccaatg acatcgcgct caaatgcaaa tactgtgaaa
     5761 aagagttttc ccataatgtg gtgctggcca attaattgcg gttggtaata aaagtctggc
     5821 tccctataat gagccagact ttttaccgct gtaataaagg agaaatcatg agcaaaacta
     5881 tcgcgacgga aaatgcaccg gcagctatcg gtccttacgt acagggcgtt gatctgggca
     5941 atatgatcat cacctccggt caaatcccgg taaatccgaa aacgggcgaa gtaccggcag
     6001 acgtcgctgc acaggcacgt cagtcgctgg ataacgtaaa agcgatcgtc gaagccgcag
     6061 gcctgaaagt gggcgacatc gttaaaacca ccgtgtttgt gaaagatctg aacgacttcg
     6121 caaccgttaa tgctacctac gaagccttct tcaccgaaca caatgccacc ttcccggcac
     6181 gttcttgcgt tgaagttgcc cgtctgccga aagacgtgaa gattgagatc gaagcgatcg
     6241 ctgttcgtcg ctaatatcta ctgaaagata atccaggtat tgcgcctggg ttatctgatt
     6301 caaccacgga gggttgaaga tggctaaacc ccgtttcact aacgaacaaa ttgctgaaat
     6361 tttacagcag tcaaaagagg gggcgtctaa taaggagcta tgtgaacact atcaatttag
     6421 cgttagcacg ctgcggcgct ggcaagaaca gcacgctgac ggcatcagaa gcgaattaaa
     6481 aaaaacagaa tccaaagcac aaatcgtctt tctggttttc tttgctatcg ctattctact
     6541 gactcttata tttgacaaac ctacaggtgg atgggtaatt ccgcctttat taatctattg
     6601 tgtttattat atccgccagt atcgcaacat ctctggcaga catattaaaa aagaagatat
     6661 ctatctctct cgttcagtaa ataacagcta tagcgccctt tataatttga gctggacgtt
     6721 tatttgtttt tttatttttg cagtaattta tttttttatt caggtgtttt cgtaaaagac
     6781 tgacctgaaa gagtatttca ggccagcctc atcacttgct tactgccagc cgtaacgacg
     6841 gctataaaat cctttcacca actgggttaa cgtcatatac cctgccaaaa tcgcaaccag
     6901 ccacgggaaa tagcttaacg gcagcgcctg cagctgcaga taactggcca gcggtgaaaa
     6961 cggcaatgcg atcccgacaa tcatcacgat cacggtcatg atcattaacg gccacgatgc
     7021 acagctctga ataaacggca cacggcgggt gcggatcata tgcacaatca gcgtttgcga
     7081 cagtaagccc accacaaacc atcccgactg gaacagcgtt tgcgtttccg gcgtgttggc
     7141 atggaatacc caccacatca ggcaaaacgt cagaatatcg aagatcgagc tgatcggtcc
     7201 gaagaagatc ataaagcgcc ccagatccgc cggattccaa cgctgcggct tttgaatttg
     7261 ctcgtcgtcg acgttatcaa acgggatcgc cacctgtgac acatcataca gcaggttctg
     7321 aataagcaag tgtaacggca acatcggcag gaagggcaag aaagcactcg ccaccagcac
     7381 gctgaacaca ttaccgaagt tggagctcgc cgtcattttg atgtatttca gcatgttggc
     7441 gaaagtgcga cgtccttcaa taaccccctc ttccagcacc atcaggcttt tttccagcag
     7501 gatgatatca gccgcttcac gggcaatatc taccgcgccg tccacagaaa tgccgatatc
     7561 cgcagcgcgt aaggcgggcg catcattaat accatcgccc ataaagccaa ccacatgccc
     7621 ttcgcgcttc agtaaggtca caatgcgttc tttatgcatc ggcgtcaggc gggcaaacag
     7681 cgtggtgcgc tgtgcgagat ttgccagttc gtcgtcagat agcgtttcaa tatcactacc
     7741 aatgaccacc tctcccgcgt ccagccccac ttcatggcac actttcgctg caactaactc
     7801 actgtcgcca gtaaggattt ttacggtgat tccactcgct tttaatgcct tcagtgccgg
     7861 agcggttgtc tcttttggcg gatcaaggaa agcaatatat ccttcgagga tcaggtcgga
     7921 ttcatccgcc cgctggtaat ctccttcacg cgctggcagg tatttcgtcg ccaccgcaac
     7981 cacgcgcagc ccctgacgat taagcgtatc agtaacccgc ttaatcttac gcagcatgat
     8041 gtcatcgagc ggcacaatct cgccattgtg acgcacctgc gaacacacat tgaggatttc
     8101 ctgcaatgca cctttgcaaa ccagctgatg gtgctcggta ttttctgcca ctaccaccga
     8161 catccggcga cgctcgaaat caaacggaat ctcatcaatt ttttgccaac gactggccag
     8221 cgagcgcgct gactcttcat ccgtaccttc gagcaccgct gtatcgagca ggtttttaag
     8281 tccggtctga taatgactgt tcaaccacgc gctatgcagc acgcgttcgc tggttttacc
     8341 ggagatatcg gtatgattct ccagcacaat tttgtcctgt gtcagggtgc cagttttatc
     8401 agtgcacaga atatccattg cgccaaagtt ctgaatagca tccagatgtt tgacgatcac
     8461 tttctgtttc gacagcttta ctgcgccgcg cgccagcgtc gaggtaacaa tcatcggcaa
     8521 catttccggc gttaagccta ccgcgaccga aagcgcaaac agtgccgctt cccaccagtc
     8581 gcctttggtg taaccattga ttaacagcac caccggagcc atcaccagca taaagcgaat
     8641 cagcagcata ctgacgcggc tgatcccttg ctgaaaggca ttcggctcgc tttcctgctc
     8701 actaacacgc cccgccagtt gaccaaacca ggtattggca cctgtagcaa tcaccattgc
     8761 ttgtgcagtg ccgctcacca cggtggtgcc cataaaacac agggtgtcac actccagcgg
     8821 attgctgtgc tccggctggc gagtggtagc ggctttttct acgggcagag actcaccggt
     8881 taacgacgcc tgagcgacga acagatcccg cgcctgcaag atacgtaaat ctgccgggat
     8941 catatccccc gccgccagtt ttattatatc gccgggcacc agctggtcga tcgggatctc
     9001 cagccagcca ttttcgcctt tgtcgttaat tacgcgcaga accgtcgctg tattgctgac
     9061 catcgctttc agggcatctg ccgctttagt ggaacgtgct tcctgaataa agttcagcaa
     9121 cgtagaaata gcgaccatta gcgcgataac gcccgcggca aataaatctt ccgtggcgta
     9181 agaaatagcg ccgagaatgg tgagtaaaat attaaagggg ttgcgatagc agacccataa
     9241 atgtacccac cacggcgacg gttgttgtgc gggtaattta ttttcaccat gttgttcgcg
     9301 ggcagattcc acttccgcct gatttaaccc ttccggatgg gtgtcgaacg ttttccataa
     9361 ttcttcttcg ggcatcaccg ccattttcag gcaatgcgca cttaaggacg gcgggaccgc
     9421 cgtattcact gtctgctgcg cgccaggcaa tggatcgcga tgaaccagac gggaaggtaa
     9481 atggcgaatg agccgggtaa aaatttcttt aaacataagg agtccctccg cactgtctga
     9541 aacagtacag aaatgtcttc aggcgtggcg aagccttacc tgaaggtaat aaagctgagc
     9601 agggacgtta aaacgtcacg gccttacgca tccggtaacg ccgtgaaaag acgggcttac
     9661 cggaaaagtt ttaatctccg tcgagggaga ggcgtgggat caggttccat ataacctccg
     9721 gtaagtgaaa aaagcgccgc aaataataag gagaatccaa taatattcgt agcatctatg
     9781 gcgatataat acctgctggc aactaaacca acgataaacc agactttacc cattgctgaa
     9841 tgcacgggta aagttaggct caaataatta aacaacacgt tacaggacaa caggatgcaa
     9901 aatcggctga ccatcaaaga catcgcacgc ttaagcggcg tggggaaatc tacagtttcc
     9961 cgggtgctga ataacgaaag cggcgtgagc cagcgcaccc gcgagcgtgt tgaagcagtg
    10021 atgaatcagc atggattttc cccttcccgc tctgcgcgcg ctatgcgtgg gcaaagcgat
    10081 aaagtggtcg ccatcattgt tacccgcctg gattcgttgt cagaaaatct cgccgttcaa
    10141 accatgctgc cagcgttcta tgaacaaggt tacgacccaa tcatgatgga aagtcagttt
    10201 tccccgcaat tagttgccga acatttgggg gtgctgaaac ggcgtaatat cgacggcgta
    10261 gtgctgttcg gttttactgg cataacagaa gaaatgttag cccactggca gtcatcgctg
    10321 gttctgctgg cgcgtgacgc aaaaggtttt gcttcggtct gttatgacga cgaaggggca
    10381 atcaagatcc tgatgcaacg gctgtatgac caggggcatc gtaatatcag ttatctcggc
    10441 gtgccgcata gtgacgtgac gaccgggaag cgacgtcacg aagcctacct ggcgttctgc
    10501 aaagcgcata aactgaatcc cgttgccgct ctgccaggcc ttgctatgaa gcaaggctat
    10561 gagaacgtag caaaagtgat tacgcctgaa actaccgcct tactgtgcgc aaccgacacg
    10621 ctggcacttg gcgcaagtaa atacctgcaa gagcaacgca tcgacacctt gcaactggcg
    10681 agcgtcggta atacgccatt aatgaaattc cttcatccgg agatcgtcac cgtagatcct
    10741 ggttacgccg aagctggacg ccaggcggcc tgccaattga tcgcacaggt aaccgggcgc
    10801 agcgaaccgc aacaaatcat catccccgcc accctgtcct gatcgtttcc tgaacgataa
    10861 attgtgatct tcgctgcgtt tcgggaacgt tcccgttttt aaatttttcc gcgcaatata
    10921 ttctgcagcc aaccaaaaat gtcatctgcc atggggcttt atgatgagca aaataaacca
    10981 aacggatatc gatcggttga ttgaactggt cggcgggcgc ggcaatattg cgacggtgag
    11041 ccactgtatt actcgcctgc gctttgtcct caaccaaccg gccaatgcca gaccgaaaga
    11101 aattgagcaa ctccccatgg tgaaaggctg tttcaccaat gccgggcaat ttcaggtggt
    11161 gattggcacc aacgtgggtg attactatca agcactaata gcgtcaaccg gacaggcgca
    11221 ggttgataaa gagcaggtaa aaaaagccgc ccggcagaat atgaaatggc atgagcagtt
    11281 gatctctcat ttcgcggaga tcttcttccc gttgctgccc gcgttgatta gcggcggttt
    11341 gatcctcggt tttcgtaatg tgatcggcga tttgcccatg agcaacggtc aaacgctggc
    11401 gcaaatgtac ccttccctgc aaacgatcta cgattttctg tggttgatcg gtgaagcgat
    11461 cttcttctac ctgccggtcg ggatttgctg gtcagcggtg aaaaaaatgg gcggcacgcc
    11521 gatccttggt atcgtgcttg gcgtgacact ggtttccccc cagctgatga acgcttatct
    11581 gctcgggcag cagctgccgg aagtgtggga ctttggcatg ttcagcatcg ccaaagtggg
    11641 ctatcaggcg caggtgatcc cggcactgtt agccgggctg gcgctgggcg ttattgaaac
    11701 tcgccttaaa cgcatcgtac cggattacct ctatctggtg gtggtgcccg tctgttcgct
    11761 gatcctcgcg gtgttcctcg cccatgcgct gattggtccg tttggtcgca tgattggcga
    11821 tggcgttgcc tttgcggtac gtcacctgat gaccggcagc tttgctccga ttggtgcggc
    11881 attgtttggc ttcctgtacg cgccgctggt gatcaccggc gtacaccaga ccacccttgc
    11941 tattgatttg cagatgattc aaagcatggg tggcacgcca gtgtggccgc tgattgcgct
    12001 gtcgaatatc gctcagggct ccgccgtgat aggcattatc atttccagcc gcaagcacaa
    12061 tgaacgcgag atctccgtgc ctgccgctat ctccgcctgg cttggggtca ctgagcctgc
    12121 aatgtacggc atcaacctga aatatcgctt cccgatgctg tgcgcgatga ttggttctgg
    12181 tctggcagga ttactatgcg gcctgaacgg cgttatggcg aatggtatcg gcgtaggcgg
    12241 cctgccggga attctctcga ttcaaccgag ctactggcag gtatttgcgc tggcaatggt
    12301 tatcgccatc atcatcccga ttgtactcac ctcgtttatc tatcagcgga aataccgcct
    12361 gggcacgctg gatattgttt aattttcttc ggggcgcaat tgcgccccct cgcatttgca
    12421 ggaataacgt aatgactaat cttccccact ggtggcaaaa cggcgttatc taccagattt
    12481 atccaaagag ttttcaggac accacgggta gcggcaccgg cgatttacgt ggcgttatcc
    12541 aacgcctgga ctatctgcat aaactgggcg ttgatgccat ctggctaacc cccttttata
    12601 tctctcccca ggtcgataac ggttacgacg tagcgaacta tacggcgatt gatcccacct
    12661 acggcacgct ggacgatttt gacgaactgg tgacgcaggc aaaatcgcgc gggattcgta
    12721 tcattctcga tatggtgttt aaccatacct ctacccaaca tgcctggttt cgcgaggcgc
    12781 tgaacaaaga aagcccttac cgccagtttt atatctggcg cgatggcgaa ccagaaacgc
    12841 caccgaacaa ctggcgttca aaatttggcg gtagtgcgtg gcgctggcat gcggaaagcg
    12901 aacagtacta tttgcatctc tttgcaccag aacaggcgga tctcaactgg gagaatccag
    12961 cggtacgcgc agagctgaaa aaagtctgtg aattctgggc cgatcgtggg gtcgacgggt
    13021 tgcgcctgga tgtggtgaat ctgatctcca aagacccgcg tttccctgaa gacctggatg
    13081 gcgacgggcg tcgcttttac accgacgggc cacgagcaca cgagtttttg cacgagatga
    13141 accgcgatgt gtttacgcca cgcgggttaa tgaccgtagg tgaaatgtcc tccaccagcc
    13201 ttgagcattg ccagcgatac gcggcactga caggcagtga attgtcgatg acctttaatt
    13261 ttcatcacct gaaggtcgat tatcccggtg gtgaaaaatg gacgctggct aaacctgact
    13321 ttgtggcgtt gaaaacattg ttccgccact ggcaacaagg aatgcacaac gtggcatgga
    13381 atgccttgtt ctggtgtaac cacgatcagc cgcgcattat ttctcgcttt ggtgatgaag
    13441 gtgaataccg cgtgcctgcg gcaaaaatgc tggcgatggt gctgcatggc atgcagggaa
    13501 cgccgtatat ctaccaaggc gaagagattg gcatgaccaa cccgcatttc acgcgcatta
    13561 ctgactatcg cgacgtggag agcctcaata tgtttgccga gctgcgcaac gatggtcgtg
    13621 atgccgacga gttattggca atcctcgcca gtaaatcccg tgacaacagc cgcacgccca
    13681 tgcaatggag caacggcgat aatgccgggt ttacggctgg cgaaccgtgg attggcctgg
    13741 gcgataacta tcaacaaatc aacgtagaag ccgcgctggc cgatgactcc tcggtgtttt
    13801 acacctacca aaagttaatc gcactgcgta agctagaagc catcctgaca tggggcaatt
    13861 accaggatct gctgccaaac agccctgtat tgtggtgcta tcgccgtgaa tggaaggggc
    13921 aaaccttgct ggtcattgcc aaccttagcc gtgagatcca accctggcag ccagggcaaa
    13981 tgcgcggcaa ctggcagctt gtgatgcata actacgaaga agcctcacca caaccctgtg
    14041 ccatgacttt acggcctttt gaggctgtct ggtggttaca gaagtaagta tccgctaagc
    14101 cccgggaatg cctgtcattc cggggttttg tctgtcagca tgttgttttt gttgattttt
    14161 caaccagcaa attcattaaa aaatttacat aacgctgtag cgcccgtcat ccgtacgctc
    14221 tgctttttac tttgagctac atcaaaaaaa gttcaaacat ccttgatgca aagcactata
    14281 tatagacttt aaaatgcgtc ccaacccaat atgttgtatt aatcgactat aattgctact
    14341 acagctcccc acgaaaaagg tgcggcgttg tggataagcg gatggcgatt gcggaaagca
    14401 ccggaaaacg aaacgaaaaa accggaaaac gcctttccca atttctgtgg ataacctgtt
    14461 cttaaaaata tggagcgatc atgacaccgc atgtgatgaa acgagacggc tgcaaagtgc
    14521 cgtttaaatc agagcgcatc aaagaagcga ttctgcgtgc agctaaagca gcggaagtcg
    14581 atgatgccga ttattgcgcc actgttgccg cggttgtcag cgagcagatg cagggccgca
    14641 accaggtgga tatcaacgag atccagaccg cagttgaaaa ccagctgatg tcgggtccat
    14701 acaaacaact ggctcgtgct tacatcgagt accgtcacga tcgcgacatt gaacgtgaaa
    14761 aacgcggtcg cctgaaccag gagatccgtg gtctggtcga gcagaccaac gcctcgttac
    14821 tcaacgaaaa cgccaacaaa gacagcaagg tgattccaac ccagcgcgac ctgctggccg
    14881 ggatcgtggc taaacactat gcacgccagc acctgctgcc gcgtgacgtg gtgcaggcac
    14941 atgagcgtgg cgatattcac tatcacgatc tcgattactc gccgttcttc ccgatgttca
    15001 actgcatgtt gatcgacctg aaaggcatgc tgacccaggg ctttaaaatg gggaacgccg
    15061 agattgaacc gccgaagtcg atctctacgg caaccgcggt aactgcgcag attattgctc
    15121 aggtcgccag ccatatttat ggcggcacca ccattaaccg tatcgatgaa gtgctggcgc
    15181 cgtttgttac cgccagctac aacaaacatc gcaagaccgc agaagagtgg aacatcccgg
    15241 acgccgaagg ctacgcaaac tctcgtacca tcaaagagtg ctacgatgcc ttccagtcgc
    15301 tggagtacga agtaaacacc ctgcacaccg ccaacggtca gacgccgttt gtaacctttg
    15361 gttttggtct gggcaccagc tgggaatcgc gcctgattca ggaatcgatc ctgcgtaacc
    15421 gtatcgcagg cctcggtaaa aaccgtaaaa ctgcggtgtt cccgaaactg gtgtttgcga
    15481 ttcgcgatgg cctgaaccat aaaaaaggcg atccgaacta cgacatcaaa cagctggcgc
    15541 tggagtgcgc aagcaagcgc atgtatccgg atatcctgaa ctacgatcag gtagtgaaag
    15601 tcaccggttc gtttaaaacc ccgatgggct gccgcagctt cctcggcgtg tgggaaaatg
    15661 aaaacggcga gcagatccac gatggtcgta acaacctcgg cgtgatcagc ctgaacctgc
    15721 cgcgtattgc tctggaagca aaaggcgatg aagccacctt ctggaagctg ctggatgaac
    15781 gtctggtgct ggcacgtaag gcgctgatga cccgtatcgc tcgtctcgaa ggcgtgaaag
    15841 cgcgcgtggc cccgatcctc tatatggaag gtgcttgtgg cgtgcgtctg aatgctgacg
    15901 atgatgtttc tgaaatcttc aaaaacggtc gtgcgtctat ttcgctgggt tacatcggca
    15961 tccacgaaac cattaacgcg ctgttcggcg gcgagcatgt atatgacaac gagcagcttc
    16021 gcgcgaaagg tatcgccatt gttgaacgtc tgcgtcaggc agtggatcag tggaaagaag
    16081 aaacgggtta tggtttcagt ctctacagca cgccgagtga aaacctgtgc gaccgcttct
    16141 gccgtctcga tactgctgag tttggcgtgg tgccgggcgt gaccgataaa ggttactaca
    16201 ccaacagttt ccacctcgat gtggagaaga aggtgaaccc gtacgacaag atcgactttg
    16261 aagcgcctta cccgccgctg gcgaacggtg gtttcatttg ctacggcgag tatccaaaca
    16321 ttcagcacaa cctgaaggcg ctggaagatg tctgggatta cagctatcag catgtaccgt
    16381 attacggcac caatacaccg attgatgagt gctacgagtg tggctttacc ggtgagttcg
    16441 agtgcaccag caaaggcttc acttgcccga aatgtggtaa ccatgacgcc tcccgtgtgt
    16501 cggtaactcg ccgcgtgtgc ggatatttag gtagcccgga tgcacgtccg tttaacgctg
    16561 gtaagcagga agaagttaag cgccgcgtta aacatttggg gaatgggcag ataggttaat
    16621 cttcactctg cgctaagcaa atgccggatg cggcgtaaac gccttatccg gcctacattt
    16681 ggcaacggct gtaggcctga taagacgcga cagcgtcgca tcaggcattg attaccggat
    16741 gcagcctaca cgcggactga taagccaggc gaaataatga attatcatca gtactatcct
    16801 gtcgacatcg tcaacggccc cggcactcgc tgcaccctgt ttgtctccgg gtgtgttcat
    16861 gaatgccccg gttgctataa caaaagcaca tggcgggtaa attctggtca gctatttacc
    16921 aaagcaatgg aagaccagat cattaacgat ctgaatgaca ctcgtatcaa acgccagggt
    16981 atttccctct ccggcggcga tccgctgcat ccgcaaaacg tgccggatat tctgaaactg
    17041 gtacaacgca tccgcgccga gtgtccgggt aaagacatct gggtgtggac aggctataaa
    17101 ctcgacgaac tcaacgctac gcaaatgcag gttgttgatt tgattaacgt gctggtcgac
    17161 ggcaagtttg tgcaggattt aaaagaccct tccctgatct ggcgcggcag cagcaatcag
    17221 gtggtgcatc atttgcgatg ataagttccc tgttacctga gtgcaggcaa cagggaaatg
    17281 aggaattaac gatacttctg gtgataggcg ttgcgggtcg ttttcagttg ctctgcggcg
    17341 gcctgcgctt cttttacttt accttcattt gccagcttca gcgcgtcgtc aatctgaccg
    17401 accagaatgt cgaaaccgtg gcggaaatct ttcatttccg ggctgtccgg tgatttatct
    17461 tcgagcttcg gcggcgttgc tttttgcgca tccagcgctg cggcgcgcat catcgttaac
    17521 gcgtctttga cttgcgccgc gttatccgct ttttcgatca cttttaaatt gtcgttgagg
    17581 gtttccatat tgtcttcaag atcagcggca aacgacgcag aactgaatac caacgaggag
    17641 actgcaagaa tagctaacag gcttttacgc attgctcact tcctttttta tttacacttc
    17701 atccttcaca ctgcctcatc gacggctacg ctcattcgcc cgggtcacgt actgcgagta
    17761 cgctccccgg ccctcattca ctttccgtct cgatgcagtg cgaatgattt tgtgtaattt
    17821 tttagtttat agcgcggcag gtcgcgccag ttttttactg cccggcgatt ttcatctccg
    17881 gcaacagcac agaaccacac tgtatattac tgcgtgtttc aatatcgtta ccgacggtga
    17941 caatattgcg ccacatatct tttaaattac ctgcgatggt gatttcacta accggatact
    18001 gaatttcgcc attctctacc cagaagcccg cagcaccacg ggaataatca ccagtaatgg
    18061 cactcacgcc ctggcccatc agctccgtca ccaccagccc ggtgcccatc tctttgagca
    18121 tttgttcgaa gcttagccct tgtccggcaa tccgccagtt gtgaataccg cccgcatgtc
    18181 cggtgctttt cagccccagt ttccgcgccg agtagctggt cagcagccac tgagtcagga
    18241 tgccatcttt aataatatcg cgacgctcgg tgcgcacacc ttcgctgtcg aatggcgtcg
    18301 acgccagccc tttcagcaga tgcggatgct cttcaatggt cagccagtcc ggcagaattt
    18361 gtttacccag cgaatccagc agaaaggtag atttacggta aaccgagcca cccgctatcg
    18421 cccccaccag atggccaaaa agcccggttg ccacttcatt ggcaaaaatg actggcgctt
    18481 tcatggtgga gagtttacgc ggtgacaggc gcgataaagt acggcgagca cagtcggccc
    18541 caacccactc tggcgtttgc agatcgctca tcgcacgacc aatggtgtag gcgtaatcac
    18601 gctccatatc gccattttct tcggcaatta cacagctgga gagtgaatga cgcgtagagc
    18661 agtaaccctg caacatgccg tggctgttgc caaaaacttt gacaccgtag tggctgttaa
    18721 aactgccgcc ttcggtattg gtaatgcgtt tgtccgcctg caatgccgcc tgttctgcgc
    18781 gggccgccag ttcaatggca tcatccgggg aaacttcagc agggtggaac aagtcgagat
    18841 ctggtgcgtc aaaggccagc agctctttgt ctgccaggcc ggcacaggga tctggcgagg
    18901 tataacgggc aatatccagc gccgcctgta cagtacgggc aatggcctgc gggcttaaat
    18961 cggtggatga tgcgctacct ttgcggttct ggtgataaac agtgatcccc agcgcgccat
    19021 cgctattgaa ttcaacattc tccacttcac cataacgcgt gcttacgcta atgccggtgg
    19081 tcttgctgac ggcaacttcc gcaccgtccg atttgcctga ggccaactcc agcgcagtgg
    19141 aaactgcttc ttccagaatc ttgcgctgcg cttcaacttg agagattact ttcattgcaa
    19201 gtgccataat gtagagagtt tctctgaagt ctaacagaga accgtttttc agtgcgcatc
    19261 ttaactggta acattagcct ctttttttaa ggagcctgag atgactaagc agcccgaaga
    19321 ctggctcgac gacgttcccg gtgatgacat cgaagacgaa gacgatgaaa ttatctgggt
    19381 cagtaaaagt gaaattaaac gtgatgccga ggagctaaaa cgccttggcg cggaaattgt
    19441 tgatctgggg aaaaacgcgc tggataagat cccgttagat gcggatctgc gcgcggctat
    19501 tgagctcgcc cagcgtatta agatggaagg tcgccgccgc cagttgcagc tcatcggtaa
    19561 gatgctgcgc cagcgcgacg tagagcctat tcgtcaggcg ctggataagc tgaaaaatcg
    19621 ccacaatcag caggtggtgc tgttccataa actggaaaac ctgcgcgatc gtctgatcga
    19681 tcaaggtgat gacgccatcg ctgaagtatt gaatctgtgg cccgatgccg atcgtcagca
    19741 gctgcgtact ctgatccgta acgcgaagaa agagaaagaa gggaataagc cgccgaaatc
    19801 cgcacgccag attttccagt atctgcgcga actggcggag aatgaagggt aattttcgtt
    19861 gttgatgtga gtatggtttg ccggatgcgg tgcacctgta ggcctgataa gacgcatcaa
    19921 gcgtcgcatc aggcactatg ctcaaatgcc ggatgcggcg tgaacgcctt atccggccta
    19981 cgcacactat atcaggctga ggccgaatta ctgcgcggct tccgccttct tcgccagacc
    20041 atccagcagt ttctgatgga tgccaccaaa accgccgttg ctcatgacca gaatatggtc
    20101 gccaggctga gcggttttca ccaccatatc tgccagcgta tccacatcgc cactccagtg
    20161 tgcaggctga acgcaggctt ctgccacttc tgccacctgc cacggaatat gcgccggttg
    20221 cagcaggaag acttcatcgg cacgacctaa tgaaggtgcc agatcgtctt tgcagatccc
    20281 cattttcatg gtatttgagc gcggttccag cacagcaata atgcgcgccg taccaccaac
    20341 tttgccacgc agcgccgcca gcgttgccag aatcgccgtc gggtgatggg caaaatcgtc
    20401 atataccgtc acgccattcg cttcaccacg caactccaga cggcggcgag cgttaataaa
    20461 cgaacccagc gcgttagcgg catctgcagg cgctacacca acatggcgag ccgctgcaat
    20521 cgccatcagg ccattgtgca tattatgttc gcctaccagc gaccatttca cttcgcccac
    20581 tttttcgcca tcaagcaaga cttcccattc ggaagcatcg gtggtcagct ttttcgcctg
    20641 ccagtgcccc tgctcaccca ccagctcctg ctcgctccag cagcccatcg ccatggtctg
    20701 tttcaggttg atgtcatttt ccggccagat aatacggccc tgccccggaa cgatacgcac
    20761 cagatggtgg aactgtttct ggatcgcttt caggtcgtca aagatatcgg cgtgatcgaa
    20821 ctcaaggttg ttgaggatca gcgtacgcgg gcagtaatgg acaaatttag agcgtttgtc
    20881 gaagaaggcg cagtcgtact catccgcttc gataacaaag aagtcgcttt cgcccagacg
    20941 cgccgaaacc tcaaagttcc ccggcacacc gccgattaca aagcccggtt tgtaaccaca
    21001 ctgttccaga atccaggtcg ccattcccgc ggtggtggtt ttgccgtgtg tacctgcaac
    21061 ggccagcacc cagcggtcgc gcagcacaaa atcgtgcagc cactgtggac ctgacatata
    21121 agggatgttt ttttccagta ccgcttccac acacggattt ccacgggtca tggcgttgcc
    21181 aataatcacc agatccggct gcggatcgag ctggctggca tcgtaaccct gaatcagttc
    21241 aatgccttgc ttctcaagta aggtgctcat cggcggatac acattggcgt ccgaacccgt
    21301 tacttcatgg cctaactggc gcgccagcat cgccagaccg cccataaacg tgccacaaat
    21361 tcctaaaata tgaatgcgca tacgtcacta tcctttttta atctgccgct cattttacgc
    21421 atatgtcggg caagtgagaa acgcatttca ggataatccg caatttgctg gcgcgattca
    21481 cctgagcgca acattccgat tatttgttaa gattgttgcg gtcgctttac tccataaaca
    21541 ttgcagggaa agttttatga aaacgttagg tgaatttatt gtcgaaaagc agcacgagtt
    21601 ttctcatgct accggtgagc tcactgcttt gctgtcggca ataaaactgg gcgccaagat
    21661 tatccatcgc gatatcaaca aagcaggact ggttgatatc ctgggtgcca gcggtgctga
    21721 gaacgtgcag ggcgaggttc agcaaaaact cgacttgttc gctaatgaaa aactgaaagc
    21781 cgcactgaaa gcacgcgata tcgttgcggg cattgcctct gaagaagaag atgagattgt
    21841 cgtctttgaa ggctgtgaac atgcaaaata cgtggtgctg atggacccac tggatggctc
    21901 gtccaacatc gatgttaacg tctctgtcgg taccattttc tccatctacc gccgcgttac
    21961 gcccgtcggc acgccggtaa cggaagaaga tttcctccag cctggtaaca aacaggttgc
    22021 ggcaggttac gtggtatacg gctcctctac catgctggtt tacaccaccg gatgcggtgt
    22081 tcacgccttt acttacgatc cttcgctcgg cgttttctgc ctgtgccagg aacggatgcg
    22141 cttcccggag aaaggcaaaa cctactccat caacgaagga aactacatta agtttccgaa
    22201 cggggtgaaa aagtacatta aattctgcca ggaagaagat aagtccacca accgcccgta
    22261 tacctcacgt tatatcggtt cactggtcgc ggatttccac cgtaacctgc tgaaaggcgg
    22321 tatttatctc tacccaagca ccgccagcca cccggacggt aaactgcgtt tactgtatga
    22381 gtgcaacccg atggcattcc tggcggaaca agcgggcggt aaagcgagcg atggcaaaga
    22441 gcgtattctg gatatcatcc cggaaaccct gcaccagcgc cgttcattct ttgtcggcaa
    22501 cgaccatatg gttgaagatg tcgaacgctt tatccgtgaa ttcccggacg cgtaatctcc
    22561 tgcgcctctc cacacggaga ggcgttttta tcgctgcgca atgttgactc ttgtcactgg
    22621 cgaactctga cgattctccc acagcaccgt taatccacgc tgtaatgcta taaaaataaa
    22681 caacaaaata ccgatggcga ttttcgtcca ccatgagctc agcgtgccat caaagtttat
    22741 gtaagtctga atcagtccct gaatcgccac gccaaaaagc gtccctaata ccgttccaac
    22801 gccaccgctc aaaagcgtac cgccaattac cactgaggcg atagcatcca gttccacacc
    22861 tacgcccgcc agcgcatatc cggcctgggt ataaatcgag aagacaatcc ccgccagcgt
    22921 tgccagtccg gtggagagca tataaatgcg aatagtggtg ctgcgggtag aaatccccat
    22981 caggtttgcc gacgttgcgt tgccgccaat ggcgtatacc tgattaccaa aacgggtacg
    23041 atgcgcgagg aatatgccga taaccaccac cgccagcatc agcagtccca tcgcacttaa
    23101 gcgaccgccg ccagggattt tccacgcaag gcttgagagc gtgtcataaa tgggatggtt
    23161 tatcggaatc gactcttccg aaacgagata gctgacgccg cgcaaaaaga acattcctgc
    23221 caaagtaatg ataaacgccg ggatcttcag ggcgtcgatc aaaagcccca taaatgcgcc
    23281 aaaggcacag cccattacca gcaccagcgg aaatgccagc aacggcgaga ggccgaagtc
    23341 gccaatcact tttgccagaa acacgccggt aaaagcgatc accgaaccga cggagagatc
    23401 gatcccaccg gagaggatca caaaggtcat gccaacggca atgatcccaa gaaaggcgtt
    23461 atcggtcagg atattgcaga tcactcttgt ggaagcaaaa ccgggaaact gcgtcaggca
    23521 gtagagatag cccagcacaa agacgccgat ggtgatcatc agtggcaaat tacgttttat
    23581 cacggctacg tactccttta atcagactga taaagcgttg cgactggaca atcagcacac
    23641 aaagcaccac caccgctttt acgacctggt tcatctccgg cggaaagcca gaaagtaaaa
    23701 ttccggtatt cattccctga ataatcagcg cccccaccac cgaaagtagc aggttaaagc
    23761 gcccgcccat cagcgatccg ccgccaatca ccaccgcgag aatggcgtcc agctccagcc
    23821 ataacccggc gttattggca tcggcaccgc gaatatccgc cgccacgata atgcccgcaa
    23881 tcgccgcaca tagcccgctc aacacgtagg tgagcatgac gatgattcgc gtgtttaccc
    23941 cggcattttt tgccgcccga atgttgatac caacggcttc gataaacatc cccagcgccg
    24001 ttttgcgggt caacagccag aacaggagaa gcgtcagcac cgcgataatg accggcgttg
    24061 gcaggaacaa cagcgatccg ctgccaaacc atgagagatc cggcgagtta aacgtgacga
    24121 tctggccgga ggtgatcagt tgcgccacgc cgcgcccggc gaccatcagg atcagggtgg
    24181 cgacaaacgg ctgaattttg aggatcgcta ccagtatgcc gttccacaat cccgccagga
    24241 tgccggtgcc cagggcgctt aacaaaacaa tcggcaggct gaatcccgcg accgtcatcg
    24301 cagccgttgt ggctccggcg atagccatca ccgcccctac ggagagatca atcccaccgg
    24361 tggcgatcac cagcgtcata ccaatcgcca gtaacgcaac gggagctgca cggttaagaa
    24421 tgtcgatagg gctaccgaac aaacgcccat cctgaagcac cacctgccag aaatgcgggg
    24481 ctaccaggct atcgaccagc agcaccagca atagcgccgc cagctgcgac attcccgttg
    24541 gccagcgaaa gcgccttttc ggcggcgtag tgtccgggag agattgaggc atcacactgt
    24601 tctccttacg ccgcaatggc gttcatgatc gccggaacgg aaagctctgc cagcgggatc
    24661 tccgccacct gtttgcgatc gcgcatgata atcacccgat cggcatagcc caccagctct
    24721 tccagttcag aggagatcac cagcagcgcc agaccatcgg cgcatagcgt ttcaatcagg
    24781 cggatgatct cggcgtgggc acccacatca atgccgcggg ttggctcatc gaggatcaga
    24841 aattgcggtc gggtcagtag ccaacgtgaa agcaacactt tttgctgatt gccgccagag
    24901 agaaattcaa tcggttgttc agttgaaggt gtgcgaatgc caagctggcg gataaagcgt
    24961 tcggcaatct cttgctgctc tttgcgggaa atgggtcgta gccagccgcg ctgggcctgg
    25021 agagcgagga tgatattttc ccgcaccgag gcggcggcga tgatgccatc ggttttcctg
    25081 tcttccgggc agaatccgat gcccagtact gaagcctgat gtggcgatcg cagggtttgc
    25141 ggtttgcctt tgatcaacgc cgtgccgctg tcagcaggtt tgataccgaa gatcacttcg
    25201 gcggtttcgg tacgtccgga tcccagcaag ccagccagac cgacgatctc gccggggcgc
    25261 acttcgagat caaacggtgc tatcgttcct tttttgccgt aatttttgaa cgcggcaacg
    25321 ggtttgtcgc tcaacaatgt tcgcccggca cgctgtagcg cgtgggtatc cagctcgcgc
    25381 cccagcatca tttttaccag ttcgatctgc ggtagctcgc gcgtttcccg acagcctacg
    25441 aaactgccgt tgcgtaagac agtgatccga tcgctgacct gatagacctg atcgagaaag
    25501 tgagtgacaa agatcaggct gacgccgcga tcgcgcaact gacgcatcag gccaaataat
    25561 agctccactt cctgggtgtc gaggctggcg gtgggttcat cgaggatcag cactttggcg
    25621 gagagatcga tagcccggca aatggcgacg atttgctgca tcgcgactga aaagcggtta
    25681 agcggttcgc gcacgtcgag gaagaaaccg taagatgcca tcagttcggt ggcgcgcttt
    25741 tccatctctt tacggcgtag aaggccgaaa cgtttgggtt cgcgacctat gaatagatta
    25801 tcagcaaccg acatattggg tagcaggttg acttcctgat agacggtgcc gataccgagt
    25861 tgttgtgcat gggcggtatt tttcggtgag atagcctggc cttccagcca gatggtgccg
    25921 cgatcggcgt ggtatacacc agttaatgct ttgattagcg ttgattttcc cgccccgttt
    25981 tcaccaagca gcgccatgat ttcgccacgg cgcaggctga aatcaacgtt gtctaacgct
    26041 ttgacgccgg ggaaaaattt acttaatcct tcggtgcgga ggatctcctg gtgttggtcg
    26101 gtcgtcatgg tttcccccgg tgtaaagcgt gctcccagta ggcatgataa gacgcggtaa
    26161 gcgtcgcatc aggcttcagc gcaccaccgc cggatgcggc gcgagcgcct tatccggcct
    26221 acgaatcggc atcaggcata gcaaccctca ataccccata tttttcttct tctctaactc
    26281 ttcttttgcg gtatcaggca gataaagggt ggatttggtc agggtcagct tttcaggcat
    26341 ggtgccgtct tttttgtatt tctccagcgc atcgaaagcg ggacctgcca tattcggcgt
    26401 cagttcaaca ctggcgttcg cttcgccatc aatcatcgct ttgtagatat ccggtacacc
    26461 gtcaatggaa cccgtgagga tatctttgcc cggtttcagg cccgcttctt taattgcctg
    26521 aattgcgcca atcaccatgt catcgttatg ggcgtaaacc atgcagatgt ttttgccgtt
    26581 gttttccgct ttgataaagc tctccatgac ttctttgcct ttactgcggg tgaagtcacc
    26641 tgactgcgag cggataattt tgatatttgg cgcattctta atggcttcgg caaagccttt
    26701 cttacggtca atggcgacgc tggccccaac ggtgccctgc aactccacca cgttgcatgg
    26761 tttgccattc acttctttta ccagccagtc accgatcaac ttaccttcga ggatgttgtc
    26821 ggcagtaacg gtggtcatat agagcgattt gtctttcaca tcgatggaac ggtcgagcaa
    26881 gaagaccggg atttcggcat ctttcgcctc ttttaatacc ggttcccaac ctgtcgcgac
    26941 caccggagcg ataaagatcg catccacccc ttgtgcaacg aaggaacgta ccgctttaat
    27001 ctggttttcc tgcttttgct gaccatcggc aattttcagc gtaattccgc gtttttcggc
    27061 ctcacttttc gccacattgg tttctgcggc gcgccagccg gattccgatc cgacctgcga
    27121 aaatcctacg gttaatggag cggccaacgc catagacgac atggctgccg agactgcaga
    27181 gactacaagt aaacgtttcc acatagggtt gtcctcgtcg gggtagttat tattggttaa
    27241 aagatgttcg cgaaaaaact atagacaatc ggtgatgtaa cgaattgcgt tacatcacac
    27301 ttcaaaaaag taaataaaac gttaatcaca agtttgtaat cgctttcatc tcactatgaa
    27361 aaatgcggct acggttatgg attttcctgc tctgcatacc gtcttaaaac tggcgaaaaa
    27421 ggaaaatgaa gacgaaaaca agcgaagaca ttcggcgcga gttggctata atactcggca
    27481 cttgtttgcc acatattttt aaaggaaaca gacatgagct tactcaacgt ccctgcgggt
    27541 aaagatctgc cggaagacat ctacgtggtt attgagatcc cggctaacgc agatccgatc
    27601 aaatacgaaa tcgacaaaga gagcggcgca ctgttcgttg accgcttcat gtccaccgcg
    27661 atgttctatc cgtgcaacta cggttacatc aaccacaccc tgtctctgga cggtgacccg
    27721 gttgacgtac tggtcccgac tccgtacccg ctgcagccgg gttctgtgat ccgttgccgt
    27781 ccggttggcg ttctgaaaat gaccgacgaa gccggtgaag atgcgaaact gattgctgtt
    27841 ccgcacacca agctgagcaa agaatacgat cacattaaag acgttaacga tctgccagag
    27901 ctgctgaaag cgcagatcgc tcacttcttc gagcactaca aagacctcga aaaaggcaag
    27961 tgggtgaaag ttgaaggttg ggaaaacgca gaagccgcta aagctgaaat cgttgcctcc
    28021 ttcgagcgcg caaagaataa ataagttctt ctggcgcaat aaccctgaac accgggcttc
    28081 ggttagtaag ggttttttta tgcccgcgat aaataaacac accttattcc accaccgcct
    28141 gcaagcgtaa taacgcctct tccaccacct catccgcagc cagaccaata cgctttgcca
    28201 gccgggcgcg tagatccatc atccgcacct gattcaccag caccacgccg tgcacatcgc
    28261 cttcttcgca atgtaaagga acgctaaatc cggcataacg ggcaaaattt ccgccctgcg
    28321 taatgggggc caccagcgtc attcccagtt gattaaaggc ttgaacggag agcacaagcg
    28381 caggtcgacc agcaccttgc tgttcatggc cgcttgctgg atcaaagcca accagcacaa
    28441 tgtctccccg ttcaaattca cttttcttta ccatatttcg tcacccgcag gggtggattt
    28501 accccagaca tcctgctcgc taagttccgc ggcgttcatg tcacactgtg ccagcagttc
    28561 atcaagcgag tagcgcctgg agatgggtgt cagaatcagt tggttgttgc tcacctgcgc
    28621 ttccacgctc tgccccggct gtaagttaag ttctttcatt acgatattgg gaatgaccat
    28681 acctgcactg ttcccccatc tttttatggt aatacgcatc tgcacctcct taagttatac
    28741 aaagtataac tcacggtgct acaggaaacg ttttacccct gcacatttgt gaacgccagc
    28801 agagtttttt atgcgattgc agatatggaa agcggctcgg agagaaaagc aaaagatgga
    28861 aaagttacaa aaaaaaatcg cgagatacat cgcataaaac aacgccaccc gaaggtggcg
    28921 tgcttttatt attacttatc cctgtctaac cagtcgccgc tttcaattag ctttaatcca
    28981 tcgacgggtc gttgataaac gtacatccat gcactcccgt acggcgtctg aatcaactgg
    29041 cgcgcgtatt caccgcccct ggtgcgcaag gcatcaagtt cggccagcgt ggcgttgtca
    29101 atacgataaa cttcaccgtg tactgttccg ttccccggaa ctgcgcctgg atagtggccc
    29161 aggctataca actggtagtt atcgatactg aaatcgccca gtaactgggc attggtcatc
    29221 cagtgactgt tgccttgttt gtggcgtaaa ctgccgtaga caaatattcg cattgctaaa
    29281 actcgaactg atagagcaaa tccagtgcct ggtctacacc agacacggct tccagatata
    29341 gcttaggcat caggcgataa cgtaacgtga gtgttgctat agagtcaaat atacccacgc
    29401 cgtatttcac ttgcagacct ggcaatacat agccgctgac cactacctgg gaggagtcgc
    29461 ctactccctg ggtgtcgagc gctaaattgc ttacgccaaa cgtctcgccg attttaccca
    29521 caatctggcc actttgcgca acccccaggc caatcagcat tgaggtcatc gccgcactgt
    29581 cgctctgatc gctctccagc ccttgtccac gtagcaaata agacaatgca gcttgttgcg
    29641 acatagccgg gtcagagaag atctccgctt tcggttcgtc cgccagacca gtgacgcgaa
    29701 ctccggcgat tacgtcgtct tctgtagcat acgggttacg aatagcttca atattaagat
    29761 acggttgatc tggcggacca gagaacagta gctcaccctt acgcacaatc agatcctgac
    29821 cataggcatg gaagcgccct tcagggatgt tgatctgccc gttcaggccc agcccttgtt
    29881 tgtcctgaac gacgttaaga tcgcccgtca gccgcgcttt caggccaaag gcgtcaatgc
    29941 gcacattgtt gccaacgtgg acaatcaggt tactgttaat cggaatcgac gccgttttcg
    30001 gctcttccgg ttgcaggtta tcgttaagca tcaccacatc gctggagacg cctactgcgc
    30061 tttccggcag atcgtgcacc acgatgcgcg cccacgggac atccacgcga ccatcgaggg
    30121 taaacaggtt tggtgtagcc tcgaatacaa catctggcga tacatccatt cgtaccatcg
    30181 gcggcacggt gatccgcact ttactgcctt tcgccgttac tcgtgcccgc cagttttcaa
    30241 tttggctcca gtcggcatca ccgttcaggt agatttcacc ctgctgggtc cgtacggtac
    30301 cggcaagcgt cgagcgcata ccgttaaagt tgaccgcaag ctggctcggc tgcatatcaa
    30361 acggcataaa gttgccgtcg atatccacac ccgtaacctg aagctgacca aacaactgcg
    30421 ggctttgcac atcaccaccc agacgcaagt tggcactcac catccccgct gctttttccc
    30481 cacgggtaaa gatggggttt atcatcgcaa ggttgaagtt acggatattg acgttgccac
    30541 caagattacg gcggccttgc ggatcggtca cctgcacctg tccatcaaac tggccgttat
    30601 tggtcaggcg gatggtccag cccaattcgg cacggttgtt acgcaattcc gccgtcagat
    30661 tcagtgtctg aaacgccacc ggcagcgccg catcgttgac ggtttgcgtt acctgcacgt
    30721 tacgccccga aagggtgata ctgccctgcg gcagcccctc tttcgtggtg tcccaggcaa
    30781 catccgcttt acccgtgaag ataccgctgg cctgagtggt ttctggcata aacggtttca
    30841 gcatggcgag gtcgaagcgg ttgagattca ccaccgcacg cccttcggcc cccgcatcga
    30901 tagtttgcgg cacgcacagt tccgcattcg ggttaagcca gcagtgtggc ccgatgctga
    30961 ttttttgctc cttattgcgg taatccagcg caatatcgcg ggtcagcgac cacgggccga
    31021 ccggcgtctg gaagcgggta ttgctaagag ttcccttcca gcgttcttct ttgcgatcaa
    31081 aacttcctgc cagattaagc tgcccggaaa ccggctcgcc ctgaatccgc aactgtagct
    31141 cgtgctgctt ttcgctgcct ttggcattca gggtgacgag gttgatattt acatccggct
    31201 gcgaaatttg ctcaacgcgt acgtcgagtt tcccggcgat ctgatcggtg gatttaatgt
    31261 cgccttccac gcgaacctgc gtcacggaaa gttcctgcca gcgcaggccg cgcgcggtga
    31321 tatctgccag tagttgtggc gcttccaccg tgccgcgtac tttcaccagc cctttcgctg
    31381 taccgccaag ccccggcagc gcgttatcca gccccggcgc gttgatggtg gcatcaagat
    31441 tgagatcttt tacccccagc tcgcctttca cttcggcact gtttggcccc agttccagat
    31501 gaagccctgg gatcatccac tgcatataac tgttgccttt cagcgtgccg tcaacgttca
    31561 ctttgttctg tttgacgtta ccggtcagct tcagttctgg cacctccatc tgccaggtgc
    31621 cgccgtacag gctaccgcgg gttttaatca agccattgag tttcgacggc cactccggga
    31681 tctctttggc ggtgttaatg ccgttaagcg ttagctcacc gcgccaacta atggcctgct
    31741 gccagtcgag caacgccttg agttcagttt tcccttccag cgccgcgacg gtgagtttgt
    31801 cgagattgac ctgctgttca ttacctttgg catcaagggt aatggtggct ggcgggatct
    31861 ccagtccctt cactgccgta cgcatagaga gcgtgtaatc ggtcattttg ccggtaagtt
    31921 tcagtttcag atcatccgcc tgatactgct tctcaccggt gaacggccag taaagctgtt
    31981 tgctgttcac ttccacgttg agtggcaatc cggcttccgc cagtcgcgtc tgggcgcgta
    32041 aatccatatc caccggaccg gaaaggttaa cgccaatctc cagctgttcg cgcagcgcac
    32101 cgcccacttt cagcttcagt ttttctcctt tcaacggctc cacgtttagt gtgcagttga
    32161 gggtgatatc caccggccag ttgtctgaca gctgcgccgt gccgctggcg ttgacgatcc
    32221 cttgactgga atcgatatcc agggcgtcca gtttagtatt gccgtcaatg ctgctcactt
    32281 tcagcagcat ggtgcgcacg gtgatgtccg tgtcgcccgt cacgcgtaac tgctcgcctt
    32341 taaactcttc aatgttcagg ttaagcggca aatgcacgtc ggtcatttcc ggcaataccg
    32401 ggcgagaaaa gagatctttc agcgtttcgc cgagcggctt ttcctccggc tgcggatttt
    32461 caattttcgg ttcgaccact tcttcctgcg ccacttccgc cactttcggc agagcaatca
    32521 gcaggccttt cagcgacgtc ggtttcaggg tcagggtttt ctcctgccag ttcaggccgg
    32581 aggtgaagtc catcaccgac accgtggtgt catcaatctt gatgttgacg ttgtccagcg
    32641 ccacccgtgt cagggtgatg ggatacggcg tggagagatc cagcggaccg ctatcttcct
    32701 cttcttcaac ctgttcagaa ggaggcattt ttttactgtc gatgttgacc tgaatgtctt
    32761 tcagcgccag gtcattaata caaacgctac tgttccacag gcactcaaga ccgaccgcca
    32821 gatgcagatt acccgctttt accgccacgc ctggctgctc ataacgaacg tcagacaagg
    32881 tgagatcgcg ccagccgccg gtgaccttgc caatatccag tcctggcacc cagcgttccg
    32941 ccgctttaaa tacaagatgc aggccgctgg tggtgcccac cagaaacgcc accgatccca
    33001 gcaacagtaa gataacgata accacgccga ggctgatttt tttccataaa ctcataattc
    33061 tggccccaga ccgatgtaaa actgtaaccc gtgttcgtct ttatccgcga ccggtacggc
    33121 aaaatcgagt ttgattggcc cgaccggcga ttcccagcgc acgccgaccc cggtaccggt
    33181 tttaaagtcg ctgcggcgaa tatcgcttac cgcttcgcca ctatcgacaa acaccgcgcc
    33241 ccaccatttt ccggtcacgt tgtactggta ttccagcgat ccggttatca acttcgaggc
    33301 ccctttcagg tcaccgttgg cgtatttcgg ggcgatagat ttgtatttgt agccgcgaat
    33361 actgcgatcg cccccggcga agaaacgcag atccggcggt actttgtcga aatcaccggt
    33421 ttcaatccag cccagcgtgc cgcgtgtaac gaaacgatgg cgatcgtaca gtgtgcggat
    33481 ccagacgttc tgcgcctgga aaacggagaa atcgacatct gaaccccagg ccgtgttgga
    33541 gtagtcgata gagtagcgtt gcgagtcgcc ccaggttggc atcaggccac cacgagaacg
    33601 cgtgcggcta atcatcaccc caggataaaa cagcatcgtg gtgttggtaa tttcaccctg
    33661 ggtaaagtgg tcgaggctcc agcgcaggtt aatggcacgc tgccagccgc tggagagatc
    33721 ccagtagcga gaagccacca gcgtagtgga gtcagattcg gtatcgttca ggtcagtgcg
    33781 cttaaaaccg ccctgcacca aataatattg ttccagtgga ttcttcagca gcggcatttt
    33841 atagctgaag tcgagggtct gttccggcgc ggaaatactg gtactggtgg tcagactgtg
    33901 accataagag ttcatccacg gctttttcca cgtcgctttc acgcgcggtc ccacgtccgt
    33961 agagtaaccg accccggttt cgatggtgtt ttcagttcgc ggcgaaacca cgcccgtcaa
    34021 gggtaatact ttcgtttcgc gcgctttatc aaattgtgga gccaccacca ccgagttaaa
    34081 ccagccggta gcagaaagtc ggcggttcag ttccgccaga tctttcgatt cgtactcatc
    34141 gccctcttta aacggcacca gattttgcag gtattcatcg cggatttgtg atccttcaaa
    34201 ggtcacatgc ccaaagcggt aacgttcgcc actgttataa tcaatatccc agaaggcttt
    34261 atgcaggccg agcgcaatgc ccagctgcgc tttggtaaat tcgctatcga aataaccttt
    34321 acgcaacgca atgctggtta aggacttttt gaaattttca taatcgccct ggttcagcac
    34381 cgtgccaata gccgggcgag tatcgagcaa tttcaaatag tctttatcgg tccgcgcgcc
    34441 gccgcgcaat accacatcgg tgccgccaat taacaccggc acacctggcg tgactttggc
    34501 gatcaatacc tgccgccctt tctttggcgg tggacggaga tcaaattcaa tggtcggctg
    34561 gtaataaccc agcgctttca gaccttcgcg gatggcatca tcgacgcgtg cgcgaaagcg
    34621 acggtctggc gtcacttcat cactttcaat cgtagaaagc tgcgcacgaa cgttcttttc
    34681 cagctgtccc gataacccct cgacctgtag acggacgttc gcggcgacgg cagatccgct
    34741 taagcagagt aagcttacac agcataactg tcggatatag cgcacatttt ctcctgaata
    34801 tccttttttc ctgcccctgg aacgccgtta aacggcttaa caaaaatcca gtaatatgga
    34861 ttaaaaagca gactaaaccc caaatatttc ttatgtttta ctttagacct attcacggtg
    34921 gttattgtgt gcaaatacgc ctcttgttac aaccttaacc ccaatgaccg attttcggga
    34981 gagcgacacc atgagtttat ttgataaaaa gcatctggtt tcccccgccg atgccctgcc
    35041 tggacgtaac accccgatgc ccgtagccac gctgcatgcg gtcaacggtc actcaatgac
    35101 caatgtacct gacggaatgg agattgccat ttttgcgatg ggttgtttct ggggagtgga
    35161 gcgtctgttc tggcagttac ccggcgttta cagcaccgcc gcaggctata ccggaggcta
    35221 tacgccaaat ccgacttatc gggaagtgtg ctccggtgat acgggtcatg ccgaagcggt
    35281 acgcattgtt tacgatcctt ccgtcatcag ctatgagcag ttgctacagg tattttggga
    35341 gaatcacgat cccgcccagg gaatgcgtca gggcaatgac cacggcacgc agtatcgttc
    35401 agcgatttat ccgctgaccc cagaacagga tgccgcagct cgcgccagtc tggaacgttt
    35461 tcaggcggcg atgcttgccg ccgatgacga tcgtcacatc accacggaaa tcgctaacgc
    35521 cacaccgttt tattatgccg aagatgacca ccagcaatat ctgcataaaa acccgtatgg
    35581 ttactgtgga attggcggaa ttggcgtctg tctgccaccg gaagcatagc gttacgggta
    35641 caaatgtaga ttgttgataa agtgctcttt gtctatgccg gatgcggcgt gaatgcctta
    35701 ccaggcctac aaaatcgtcc aaattcaata tattgcaggg actgcgtagg cctgatcggc
    35761 atagcgcatc aggcagtatt gcatttatca gcagtctgaa ttttaaccct ctggcgactt
    35821 tacagtacct tacgctatac tagccactga aaatgccggt tcactttctt cgaatcggct
    35881 ttcaatgtgt atttcacaca aattaatcaa cttccccttc cgaggatctg gcctgaaagg
    35941 tcggataaga tatgttaaac agtattttag tcatactctg cttgatcgct gtaagtgcgt
    36001 tcttctcgat gtctgagatc tcacttgccg cctcacgcaa aatcaaactt aaactgctgg
    36061 ctgatgaagg caatataaat gcccaacgcg ttctgaatat gcaggaaaat cccggcatgt
    36121 tctttacggt ggtccaaatc ggtctgaacg cagttgcgat tctcggcggt atcgtcggtg
    36181 atgcggcatt ttctccagct tttcacagcc tgttctcccg ctatatgtcg gcagaactct
    36241 ctgagcaact gagctttatt ctctctttct cgttagtgac tggcatgttt atcctgtttg
    36301 cggatttaac cccgaaacgc atcggtatga ttgcgccaga agcggtggct ttgcgtatca
    36361 tcaacccgat gcgcttctgc ctgtacgttt gcaccccgct ggtgtggttc ttcaacggcc
    36421 tggcgaacat gatcttccgt attttcaaac tgccaatggt acgtaaagat gacatcactt
    36481 ctgatgacat ctacgcggta gtggaagccg gtgcgctggc gggcgtgtta cgtaaacagg
    36541 aacacgagct gattgaaaac gtctttgagc tggaatcccg taccgttccg tcttcaatga
    36601 caccgcgtga aaacgtgatt tggtttgatc tccacgaaga tgagcaaagc ctgaagaata
    36661 aggtggcgga acatccgcac tctaagttcc tcgtctgtaa tgaagatatt gaccacatca
    36721 tcggttatgt cgattctaaa gacctgctga accgcgtgct ggctaaccaa agcctggcac
    36781 tgaacagcgg cgtacaaatt cgcaacacgc tgattgtgcc ggatacgtta accctttcag
    36841 aggcgttgga aagttttaaa accgcaggtg aagacttcgc ggtgatcatg aacgagtacg
    36901 cgctggtggt ggggatcatc accctcaacg acgtgatgac cacgctgatg ggcgatctgg
    36961 tcggtcaggg gctggaagag cagattgtcg cccgtgatga gaactcatgg ctgattgacg
    37021 gcggcacccc aattgacgac gtcatgcgcg tgctggatat tgacgagttc ccgcagtcgg
    37081 gcaactacga aaccatcgga ggctttatga tgtttatgct gcgtaagatc ccgaaacgca
    37141 ccgattcggt gaaattcgcc ggctacaaat ttgaagtggt ggatatcgat aactaccgca
    37201 tcgaccagtt gctggtgacc cggatcgaca gcaaggccac cgccctttcg ccaaaactgc
    37261 ctgacgctaa agataaagaa gaaagcgtcg cgtaaccccc agaaacatca acggctcctg
    37321 aatcaggagc cgtcttatta ctgcatagca ctttggttaa gccatctctg tttgcagacg
    37381 cataacctga cggttaactt cggacatcac tgacaaatgc agcttatcct tcacttttgg
    37441 gataagaatc ttacccttat caaattcaaa agcgccaacg tccttgatgt ataaccgtcc
    37501 acggaacagg atctttacgt acttcgccac ctgaagtggg ttgtaccgtt ggaaaatttt
    37561 cattgttatc tcctgctgag tattacgcct gtgcggtgcc acaatcggcc caactattat
    37621 gaggcgcaaa ttttaatgcc tagtgactat agactatccg ggcaatgttt ccaccgcgta
    37681 taactttttt tacctaaagg ttacaattat tcagaattat ctttttaccg aagcgcggtc
    37741 ttcagtataa gcattcattt ttcatatgat ttgtgcgctt gaccgcaaac tggcatcaca
    37801 cttgcgggaa attcgataaa tagcacatat gattaaaact cagacccaag tggtcgtatc
    37861 acctgcatat cataagaagg aaacaccatg accctacgca agattctggc actcacctgc
    37921 ctgctgttgc cgatgatggc ttccgcacat cagttcgaaa ccggtcagcg agtgccgccg
    37981 attggcatca ccgatcgtgg cgagttggtg cttgataaag atcagtttag ctacaaaacc
    38041 tggaacagcg cgcagttagt gggaaaagtg cgagtactgc aacatattgc tggtcgcacc
    38101 tctgcaaaag agaaaaacgc gacgctgatt gaagcgatta aatcagcgaa gttaccgcac
    38161 gatcgttacc agaccaccac cattgttaac accgacgacg caattccggg ttccggcatg
    38221 tttgtgcgca gcagtctgga gagtaataaa aagctttatc cctggtcgca gtttattgtt
    38281 gatagcaatg gcgtcgcacg cggtgcctgg cagctggatg aaaaaagttc cgctgtagtg
    38341 gtgctggata aagacggtcg cgtgcaatgg gccaaagacg gggcgcttac tcaggaagag
    38401 gtgcagcaag tgatggacct gctgcataaa ttaattaata aatagacact ctgaacccag
    38461 gattcaggaa cgactcgcgc ggagtgtaat ccagcggttt accctgccag tcgtgaacgt
    38521 gcgctccggc agctgcggcc acagcatgtc cagcggcggt gtcccaaata ttcgttggtc
    38581 cgaagcgcgg gtacagctgc gcctgtcctt ccgccaccag gcagaatttc agcgaagagc
    38641 cgatggacgt ggtctgatgt tcgccaagct gttgcagata ctctttcagc tccgcatctg
    38701 catgggaacg gctgataacc accagcggcg ggcgcgcatc gcggacctga atctgcttgc
    38761 gcacaccgca ctcttctttc caggccttgc cttctgccgc gctgtacatt acgttcatta
    38821 ccggcgcata caccacacct aaaatcggtt tgccatggtc aatgagcgca atgttaacgg
    38881 tgaattcgcc attgcgttta ataaactctt tagtaccatc cagcgggtct accagccagt
    38941 aacgctgcca gtgctgacgg acttcccaac cgggaggatc ttcttcagaa aggaccggaa
    39001 tatccggtgt cagcgtacgt aaaccgtcca tgataacggt gtgtgcggca atatccgctg
    39061 ccgttaccgg agaattgtcc gctttgctga cgacgtccat cggtttcgtc ccgtcgtaga
    39121 cctgcataat ggcatcgcct gcattccgtg caagctggca tacttgatct aacatttctc
    39181 cacctcgtct ctgtgagcgg tgttaactta ttgttttact tataccctat cgttaatgaa
    39241 tgcgccaact gtgatagtgt catcattttc aaagcgtaaa attgtggcat tcttcactgt
    39301 tctataagta agacgtttat tctccctttt ctttcgtatt cccgatgata aaaggatgtc
    39361 cctgatgatt aagtttagcg caacgctcct ggccacgctg attgccgcca gtgtgaatgc
    39421 agcgacggtc gatctgcgta tcatggaaac cactgatctg catagcaaca tgatggattt
    39481 cgattattac aaagacaccg ccacggaaaa attcggactg gtacgtacgg caagcctgat
    39541 taacgatgcc cgcaatgaag tgaaaaacag cgtactggtc gataacggcg atttgattca
    39601 ggggagtccg ctggccgatt acatgtcggc gaaaggatta aaagcaggtg atgttcatcc
    39661 ggtttataag gcgctgaata cgctggatta tacggtcggt acactcggca atcatgaatt
    39721 taactacggc ctggattacc tgaaaaatgc gctggcggga gcgaaattcc cttatgtaaa
    39781 tgccaacgtc attgacgcca gaaccaaaca gccaatgttt acaccgtatt taattaaaga
    39841 taccgaagtg gtcgataaag acggaaaaaa acagacgctg aagattggct atattggcgt
    39901 cgtgccgccg caaatcatgg gctgggataa agctaattta tccggaaaag tgacggtgaa
    39961 tgatattacc gaaaccgtgc gcaaatacgt gcctgaaatg cgcgagaaag gtgccgatgt
    40021 cgttgtcgtt ctggcgcatt ccgggctgtc tgccgatccg tataaagtaa tggcggaaaa
    40081 ctcagtttat tacctcagtg aaattccggg cgttaacgcc attatgtttg gccatgctca
    40141 cgccgttttc ccgggtaaag attttgctga tatcgaaggg gctgatatca ccaaaggcac
    40201 gctgaatggt gttccggcgg taatgccggg catgtggggc gatcatcttg gtgtggtcga
    40261 cttacaactc agtaatgaca gcggtaaatg gcaggtgacg caggcgaaag cggaagcacg
    40321 accgatttac gacatcgcta ataaaaaatc cctcgcggcg gaagacagca agctggtaga
    40381 aacactcaaa gccgatcacg atgccacacg ccagttcgtc agcaagccaa tcggtaaatc
    40441 tgccgacaat atgtatagct atctggcact ggtgcaggac gatccgaccg tgcaggtggt
    40501 gaacaacgcg caaaaagcgt atgtcgaaca ttacattcag ggcgatccgg atctggcaaa
    40561 actgccggtg ctttcagctg ccgcaccgtt taaagttggt ggtcgcaaaa atgacccggc
    40621 aagctatgtg gaggtggaaa aaggccagtt gaccttccgt aatgccgccg atctttatct
    40681 ctaccccaat acactgattg tggtgaaagc cagcggtaaa gaggtgaaag agtggctgga
    40741 gtgctctgcc ggacagttta accagattga tcctaacagc acgaaaccac agtcactcat
    40801 taactgggat ggtttccgca cttataactt tgatgtgatt gatggtgtga attatcagat
    40861 tgatgttacc cagcccgccc gttatgacgg cgagtgccag atgattaatg ccaatgcgga
    40921 aaggattaag aacctgacct ttaacggcaa gccgattgat ccgaacgcca tgttcctcgt
    40981 tgccaccaat aactatcgcg cttacggcgg caaatttgcc ggtacgggcg acagccatat
    41041 cgcttttgct tcaccggatg agaaccgctc ggtgctggca gcgtggattg ctgatgagtc
    41101 gaaacgtgcg ggggaaattc acccggcggc agataacaac tggcgtttag caccgattgc
    41161 tggcgataag aaactggata tccgtttcga aacttccccg tcagataaag ccgcagcgtt
    41221 tattaaagag aaagggcaat atccgatgaa taaagtcgcg accgatgata tcgggtttgc
    41281 gatttatcag gtggatttga gtaagtaaaa cacttctttt tcggcctata aatcatcaac
    41341 cgcatccggc atttattggc ggatgcgatg ctggcgcata ttatccgccc tacaagccat
    41401 gcaccgtaga ccagataagc tcagcgcatc cggcagttat gccgcacgtt catcccgcac
    41461 cgccaacacc tcgggcaaat tcaactcaat ccagtccgcc agtgcagcaa ccttttcgct
    41521 cacctgctcg cccagcggcg tgaggctgta ttcaacatgc ggtggcacca ccggatacgc
    41581 gatacggtta agaaaaccat cctgttctaa cgcctgtaac gactgcgcaa gcatcttttc
    41641 gctcaccccg ccgattttgc gccgcaggtc gctaaagcga tgagtacctt cgcgtagcgc
    41701 caccagaatc aacacccccc aacggctggt gacgtgtttc aacacctcgc gcgacgggca
    41761 ctgttccgca aagagattac cctctttcag ttgttgcgac aggctaacct gactcatttc
    41821 atacttacct ttttgtacgt acttactaaa agtaagttta ggtgttagcg tatttaaact
    41881 caagacaaaa cgatggagac ttcccatgat cgctattact ggtgccactg gccaacttgg
    41941 tcactatgtt attgaatcct tgatgaaaac ggttcctgcc agccaaatag tggctatcgt
    42001 tcgtaatccg gcaaaagccc aggccctggc agcacaaggc attaccgtgc gtcaggctga
    42061 ctacggcgat gaagccgcac tgacatctgc acttcaggga gtggaaaaac tactgctgat
    42121 ctcttccagc gaagtgggcc agcgtgcccc gcagcatcgc aatgtgatta acgcggcgaa
    42181 ggcggcgggc gtgaaattta tcgcttatac cagtctgctt cacgcggata aatctccgct
    42241 cggcctcgcc gatgagcaca tcgagacgga gaaaatgctg gctgattctg gcatcgttta
    42301 caccctgctg cgcaacggct ggtacagcga aaactacctc gccagcgcac cggcagcact
    42361 ggagcacggc gtatttatcg gtgccgcggg cgatggcaaa attgcctcag caacgcgggc
    42421 agattatgcg gcagctgcgg cacgcgtgat tagcgaagcc ggtcacgaag gcaaggttta
    42481 cgaactggcg ggcgatagtg cctggacgtt gacacagtta gcggcagagc tgaccaaaca
    42541 gagcggcaaa caggttacct atcaaaatct gagcgaagcc gatttcgccg cggcactgaa
    42601 aagcgtcgga ctgcccgacg gactggcgga tatgctggcg gattctgacg ttggcgcatc
    42661 gaaaggcggt ctgtttgatg acagcaaaac gcttagcaaa ttgattggcc gcccaacgac
    42721 aacgttagcc gaaagcgtaa gccatctttt taatgttaat aactagttaa ttaaagtggc
    42781 atcctcccgc atcctctctg ataatgacgg gatgccggga gcaatcatgt ctgcttcctg
    42841 aacgtttctt ctgacagacc aatggatgcc agtaatgatt agcggcgtgc tgtacgccct
    42901 gttagcaggg ttgatgtggg ggcttatttt tgtcgggccg ttgatcgtgc cggaataccc
    42961 ggcgatgttg cagtcgatgg ggcgttatct ggcgttaggg ttaattgcgc tacccattgc
    43021 ctggctggga cgcgtgcgtc tgcgtcagtt ggcgcgtcgc gactggctta ccgccttgat
    43081 gctcactatg atgggcaacc tcatttatta cttctgcctt gccagtgcca ttcaacgtac
    43141 tggcgcgcct gtttccacga tgattatcgg caccctgccg gtggtcattc ctgtctttgc
    43201 caatctgctt tatagccagc gcgacggcaa actcgcgtgg ggaaaactcg ccccggcact
    43261 gatttgtatt ggcatcggcc tcgcgagtgt gaatattgct gagttaaacc acggactccc
    43321 cgattttgac tgggcacgtt atacctctgg catcgtgcta gcgttagttt ccgtggtctg
    43381 ctgggcgtgg tatgccctgc gcaacgcccg ctggctgcgg gaaaatcccg acaaacatcc
    43441 gatgatgtgg gcaacagcgc aggcgctggt cacactgccg gtttcactta tcggctatct
    43501 cgtcgcttgt tactggctga atacgcaaac gccggacttc tccctaccct ttggcccccg
    43561 tccgctggtg tttattagtc tgatgattgc gatagccgtg ctttgctcat gggttggcgc
    43621 actctgctgg aacgtcgcca gccagcgatt accgacagtg attctcgggc cgctgattgt
    43681 tttcgaaacg ctggcaggtt tgctgtacac ctttttactc cgccagcaaa tgccgccgct
    43741 aatgacgctg agcggtatcg cgctgttagt gattggcgtg gtcatcgcgg tcagagcaaa
    43801 accagaaaaa cctttaactg aatctgtctc agaaagttga cacgctggca gtgagttaaa
    43861 taagcctctg ctacgtaagg gttatagctt ttgccttaaa gatgcattta aaatacatct
    43921 tatcttatta agaatgaggt atcagctatg gcttatcgcg accaaccttt aggtgaactg
    43981 gcgctctcta ttcctcgcgc ttcagctctg tttcgtaaat atgatatgga ttactgctgt
    44041 ggcggtaagc agacgctggc gcgcgcggcg gcacgtaaag aactggatgt tgaggtcatt
    44101 gaagctgaac tggcaaagct cgctgaacaa ccgattgaga aagactggcg tagcgccccg
    44161 ctggcagaaa tcattgacca tatcatcgtg cgctaccacg atcgtcaccg cgagcagttg
    44221 ccggagctga tcctgcaagc gactaaagtc gagcgcgttc acgccgacaa accgagcgtg
    44281 ccaaaagggc tgacaaaata cctgactatg ctgcatgaag agctttccag ccacatgatg
    44341 aaagaagagc agatcctctt cccgatgatc aaacaaggca tgggcagcca ggcaatgggg
    44401 ccaatcagcg taatggaaag cgagcacgat gaagcgggtg aactgctgga agtaatcaaa
    44461 cacactacca ataacgtcac accgccgccg gaagcctgca ccacctggaa agcgatgtat
    44521 aacggcatta atgaactgat tgatgacctg atggatcaca tcagcctgga aaacaatgta
    44581 ctgttcccac gcgcgctggc gggtgagtga tctaaaagct ggatggcatt gcgccatcca
    44641 gcatgataat gcggttattt ccgcagttca gcagcccgct tcttaccaat aaacagccag
    44701 cccaacccca gcgcgataaa ccacagcgga gtaaccagca gcgcctggcg agtgtcatct
    44761 tccagtgtca gcaacacgac cacgaacaca aagaacgcca tacataccca gcacatcagc
    44821 ttgccgagcg gcatcttgta gatcgacttc tcatgcagat gaggacgctg tttgcggtac
    44881 accaggtacg agcaaaggat aattgtccag acgaacataa acagaatcgc ggaaacggtt
    44941 gtaatcatcg tgaacgcgcc aatcacgcta ggattcacat acaacatcac cacgccgccg
    45001 agcagacaga tacacgagaa ggtcaaccct ttcgcgggta ctgcgcgctt agaaagttta
    45061 gcgaacgctt tcggcgcaac accttcctgc gccagaccaa acagcatacg gctggtagag
    45121 aagacaccgc tgttagcgga agacgccgca gaggtcagca ccacaaagtt gatcacgctg
    45181 gcagccgcag gcaaacctac caacacaaac agctcaacga acgggctttt ctccgggact
    45241 accgaactcc acggcgtcac ggacataatc acaatcagcg cgaagacgta gaacataatg
    45301 atacggatcg gaatggagtt aatcgcgcgt ggcagtgatt tctccggatc tttggtttcc
    45361 gcagctgtag tacctaccag ctcaatcccc acgaaagcga aaaccgctat ctggaatccg
    45421 gcaaagaagc cacttaaacc tttcgggaac cagccgccgt cattccacaa atgcgcgaat
    45481 gacgcttcca caccggtcgg tgactgaaag tgcatcgcca ccatgaccag gccgacgaca
    45541 atcagcgaca cgatggcgac gattttgatc atcgcaaacc agaactccat ctcaccgaac
    45601 attttcacgg tggcaaggtt gagcgtcagc agcagcacta tcaccgccag cgaggcgacc
    45661 cagtcggaga gatcggggaa ccagaactga gcataagccg tgatcgccac cacgtctgcc
    45721 ataccggtta caacccagca gaaccagtag gtccagccgg tgaaatatcc tgcccacggc
    45781 ccgagtaaat cggaagcgaa gtcactaaaa gatttgtatt ccagattcga aagcagcaat
    45841 tcccccattg cccgcatcac gaaaaagagc ataaaaccaa tgatcatata aacgaaaatg
    45901 atcgacggcc cggcaaggct aatcgttttg ccggacccca taaacaaccc cgtaccaatg
    45961 gcaccgccaa tggcaataag ctgaatatgt cggtttgtga gattgcgccg tagcgactgt
    46021 tcagccggag cctgatcatc ggcaacgact tttacctgat ctaccatgtt tttttcttcc
    46081 tgtacctgtc tgtgttgttc aggctctacg gcctttagtc agtctatgat acgacgaatc
    46141 tgtatcggga ctcatcgata ttaggtaaca atacgcggga tgaatactaa gatttagatc
    46201 taatgttaat tttatgttta aagtgagtgt catatcacgg tgataacgcg aaacagctca
    46261 caaaaataca cgcttacggg gtctttgcaa cacaaaatag cgccgtttgg caaactgaag
    46321 ggtttattgc tgaatgcctg ctcccctctg gttcgagggg aacagaatgc gctgcttaga
    46381 gaatttccag cagttcgact tcaaacacca gggtactgaa cggagggatg gatgcgcctg
    46441 cgccgcgctc gccatatgcc agttcctgcg ggatagtcag ttcccattta gaacctaccg
    46501 gcatcagagt cagtgcttca atccagccag ggatcacgcc attaaccgga aattcagcgg
    46561 gttcaccacg agcaacggag ctgtcaaaca cggtgccgtc gatcagttta ccggtgtaat
    46621 gaacacgaac gcggtcggta cgtgccggaa ttgcgccttc accctggttg atcacgcgga
    46681 attgcaggcc agattcggtg ctattcacac cttctttttt ggcgttttct tccaggtatt
    46741 tcacaccttc agcagccatc gcctggaaac gctgacgacg aacggcatcg gcgcgctcgt
    46801 ggatttcacg cagcgcgcga tgcaccacat caaccggaac agccggatgt ttgccttcca
    46861 gcgcatcggc aatacctgca accagtgctt ctggcagcag cccttccagg ccagattcac
    46921 tcagttgttg ccctacctgc aaaccaatgc cgtagcttgc ttgcgcttcg atggtgtcaa
    46981 aagttggggt ggtcatcgta tttcctttct tagggtataa aagtagcggg cagcatatca
    47041 gccacacttc tttgggtaaa actttgtcgc aggaatgatg acaaatcccg gggaaaaaga
    47101 aacaatagca atatcccgta tgaatataat aaaggcgggt ttaccacgga catcatagcg
    47161 ttagcttatt cataggctat gattgaggaa caagacgcgg agcaggagga aaccatgccc
    47221 gggcgctttg aactaaaacc aaccctggag aaagtctggc acgcgccgga caattttcgc
    47281 ttcatggacc cgctgccgcc gatgcatcgt cgggggatca ttattgccgc cattgtattg
    47341 gtggtcggat ttctgcttcc atctgatgat acgcccaacg cgccggtggt gacgcgtgaa
    47401 gcgcagttag atattcagtc gcaatcacaa ccgccgacag aagaacagct gcgggcacaa
    47461 ttagtcacac cacaaaatga tcctgatcag gtggctccgg tcgctccaga acctatacag
    47521 gaaggccagc cagaagagca gccgcaaact acccaaacgc aaccattcca gccagatagc
    47581 ggaattgata atcaatggcg ctcttaccgt gtcgaacccg gtaaaacgat ggcgcagctg
    47641 ttccgcgacc acggactgcc cgctaccgat gtctatgcaa tggcgcaggt agaaggcgcg
    47701 ggtaagccgc tgagtaattt gcaaaacggg caaatggtga aaatccgcca gaacgccagc
    47761 ggcgtggtga cgggcttaac cattgatacg gggaataatc aacaggtcct gtttacccgc
    47821 cagccggatg gcagttttat tcgtgcgcgg tagatgaagg gtcaaccaaa agccagctaa
    47881 ggattgtctc tttgatgacc ttacgtctgg cttccagtgc ctggggggaa accatactga
    47941 tatgataaac caggccaagc gtatgctggt tgatcaaata atatccgcca agcgcagcaa
    48001 tattaatatt cacctgcaat gggtcgatat ctggcttaaa aatattgtgt ttcttccctt
    48061 catccaataa ggattccatt aactgcaagt gcgcatggtt aatttctaag agacgctgtg
    48121 attttgcata atgcacacct ttactttgat tttcactatg cacaattttt aaaaaccagg
    48181 ggttagcaat ataataatcc catgtaaaat caataatttt ctcaatggct ttttgtggcg
    48241 gaagatctga gaaatcaagg gccttttctt tttgtcgaat atcgttccag gtatattcca
    48301 gtacttcaat aaataaattt tctttattac caaagtgatg ataaaccgtc tgtttactac
    48361 accccgcagc cttaacaata ttatcgaccc tggccccttc ataaccatat tcggcaaatt
    48421 cattaaccgc gcataacagc agcttttcct tcaggctctg tacgttgctg gtatcaagat
    48481 acataacgct tccccacgac ctcttcaaga taactatctt aacccgatta attagaagct
    48541 ccagtaaaaa ggataaaaac ttgtactttc atcaagttat caaatgtcct gtgatcacta
    48601 tcgcatatca accaaccaga tggttgatct ttcttgcggc acgtcgcaag ctaattgtca
    48661 cttagaaaac aatattcaaa aaaagagagc tattgtatga aatccactcg tcctgttgcc
    48721 gtgattaccg gagccgctcg tggtattgga aagggctgtg cgctggagct tgctcgtggc
    48781 ggatttaacc tgctgattaa cgatcttccc gatgccgaca gcgtagaaaa actgcatatc
    48841 acacaacaag aatgtatagc tgaaggcgtg gaagtgattt gcttccctgc cgatgtcggc
    48901 gatctctcct tgcatgaaga gatgctcgac gcggcgcaaa accagtgggg acgtctggac
    48961 tgcctgctca acaacgcagg catttcagtg aaaaaacgcg gtgaccttct cgacctcgaa
    49021 ccagacagct tcgaccaaaa tattgccatt aacacccgcg cgccgttctt cctcgctcag
    49081 gcattcagta aacgtctgct cgcacagccg aaaccggaag cagaactgcc gcaccgctcg
    49141 attattttcg tcagttccat caacgccatc atgctggcga tgaatcgcgg cgaatatacc
    49201 attgccaaaa ccgccgtttc tgccgctgct cgtctgtttg ctgctcgcct ttgtaatgaa
    49261 caaattggcg tttatgaagt gcgccccggt ctcatcaaaa ccgatatgac tattccggca
    49321 acggcttatt acgacgaact gattgccaaa ggtctggtgc cgtggggacg ttggggttat
    49381 ccggctgata ttgcttccac tgtccgcgcg atggcggaag gtaaactgat ttacacctgt
    49441 ggtcaggcgg tcgccatcga cggcggctta agtatgccgc gcttctgagg ctgaatcatg
    49501 agagatctgt tacaacgccc ggatttattc agtatcaata ccgcaacact gggctataaa
    49561 acgccgctac ccgccattat tgatgcctgc gccgcgcgtg gtattggcgc gattgctccc
    49621 tggcgcaggg agttgcaagg tgaagatctc cagcaaatcg cccaccagct tgccgccagt
    49681 aacatgagcg tttccggttt gtgccgtagc acttactaca ccgcgccgac actggcggaa
    49741 cgcaaactgg cgattgatga taaccgccgg gcgctggacg acgcagcggt gcttaacgcc
    49801 gcctgttata tgcaggtggt tggcggtctg ccaacgggca caaaagatct gtacgaagcg
    49861 cgggaacaag taaaacaagg gattcgccag ttgctgccac attcgaaaga tgtcggcgtg
    49921 ccaattgcgc tggagccact gcacccgatg acagctgccg atcgctcttg cctgtgcacg
    49981 ttacgccagg cgctggactg gtgtgatgaa ctcgatcccg atggtgagtt tggcctcggc
    50041 gtggcggtgg atgtttatca cgtctggtgg gatccggatc tcgccagtca gatcctgcgt
    50101 gccggaaaac gcatcctcgc gtttcatgtt tccgactggt tagtgccgac cactgatctg
    50161 gtcaatgacc gtggtatgcc gggagatggc gtcatcaata ttccgtcaat tcgtcggctg
    50221 gttgaaaacg ccggatttaa tggtgctatt gaactggaaa tattctcgcc ttactggtgg
    50281 cagaaagata ttaacagcac gctggatatt agcgtcgatc gcatcgcgca ttattgttaa
    50341 ggaatgatca tgagtgacca acccgttctg ttcagcagag tggcggcttc ctgccgcctg
    50401 acattgaacc gtgaagataa atgtcatgct attaacgaag agatgatcga atctctcgac
    50461 cattatttaa atgaaattga aaacgacacc acattacgat tagtcgaatt aacggcaacc
    50521 ggcgataaat tcttttgtgc gggcggcgat attaaatcgt ggtcggccta ttcgccatta
    50581 gatatgggca gaaaatggat taaacgcggt aatgacgttt ttaatcgcct gcgaaactta
    50641 ccccaattaa ccgttgcgaa tctcaacggt cataccatcg gcggtggtat tgaactggcc
    50701 ttgtgctgcg atattcgtat tgcccgcccc ggcgcgaaat tctctaaccc ggaagtgatg
    50761 ctcggcatgg tgcctggctg gatgggcatt gaacgtgtgc ttaatcaggt tggtccggtg
    50821 gtcggtcgcc agatgctgat gctgggtaaa cgcctgacgg cgcaggaagc ccaggcggcg
    50881 aatttaattg atgaagtggt agaaaaagaa caggtggaaa gctggatggc gaaccagtta
    50941 gcgcagctgg aaaaatgcgg tccggtggcg cttgcacata ttaagcaact gattctggca
    51001 ctggaaaata aacatgccga ttacccgcac cagctactgg ccggattaat gtccgccact
    51061 caggattgcc agcaggcgac gcgcgccttt gccgaaaaaa gtagcgtaag cttccataat
    51121 caataaggaa attgtatgcg taagataaca accgccgagg cactggcggc gcaaattcag
    51181 gacggtgcga ctattgctat tagcggtaac ggcggcggta tggtggaagc ggaccatatt
    51241 ctggctgcta ttgaagcgcg tttcctgcaa accggacacc ctcgcgacct gacgctaatc
    51301 cactcgctgg gtattggcga tcgcgactgc aaaggcacta atcgttttgc gcacgcagag
    51361 atgctcaaac gcattattgc cgggcacttt acctggtcgc ccaaaatgca ggcgctggta
    51421 aaaaataaca cgattgaagc ctactgcttt cctggtggcg tgattcaggc actgctacgg
    51481 gagatcggcg ccggacgtcc ggggcttttt acccacgttg ggctgggatc gtttgttgat
    51541 ccacgcaatg gcggcggtaa gtcgaatgaa tgcactaccg acgacctggt agaactgatt
    51601 gaaatcgatg gtgaaaccaa acttcgttat cgccctttca aagtggatta cgcgattttg
    51661 cgtggcactt atgccgatcc tcgaggcaac gtcagccttg aagaagaagc gattgatatg
    51721 gatagctatt ccatggcgct ggcagcacac aacagcggcg gcaaagtgtt cgtacaggta
    51781 cgcgatgtgc tggaagctgg cgcgattgaa ccacgccggg tcaaattacc gggcattctg
    51841 gttgatggca tcgttgaaca ccgcgaacaa ccgcaaacct atcttggcgg ttacgacctg
    51901 accattagcg gtcaacatcg ccgtctgagt tctaacgacg ctattgaact ggttagtcat
    51961 ccggtgcgcc gcctgattgc ccgtcgggca gcacgcgaac tggtggctgg cgcttcaacc
    52021 aactttggct ttggtattcc tggcggtatt ccaggcgtag cgctgcgcga aggcgttcct
    52081 taccaaagtt tatggctgag cgtcgagcaa ggcgtacata acggcatgat gcttgatgat
    52141 gcgtttttcg gctgcgcccg caacgccgat gccattattc catcactcga tcaatttgag
    52201 ttctacagtg gcggagggat cgatatcacc ttcctcggca tgggagagat ggatcagtac
    52261 ggtaacgtca acgtctccca tctcaatggc aatctcattg gtcccggcgg ttttctcgaa
    52321 attgcgcaaa acgcccgtaa agtggtgttc tgcggcacgt tcgacgccaa aggtagcaag
    52381 attgatgtaa cgccagatgg cttgcatatc acccagtcag gtcaaatccc taaactggtt
    52441 acccaggtgg aaaaaatcac ttttagcgcc gcctacgcac agcaaagtgg tcaggaagtg
    52501 ttgtatatca ctgaacgcgc agtattccag ttaacggcag aaggcgttga attaattgaa
    52561 atcgcgccgg gtgtggagat tgagcgcgac attctgccgt atatggcttt ccgtccaatt
    52621 atcaagcacc cacgcctgat ggaaagtagc ctgtttacgc cgatggagga tgcatgagtc
    52681 agcttgatga caccatcctt gatgcgctga cgcacgttac tttcccaaag ggttttgcac
    52741 aggcagagcc cgcatgggtt gtcacggtag acggtgttga ttacccactc tggcaaacag
    52801 atgccctggt agtcggcagt ggtgcagcag gattacgtgc ggcagttgaa cttaaacgcc
    52861 gccagcaaaa tgtgctgatc gctaccgccg ggttatatat ggggacgtcg gcatgttctg
    52921 gttccgataa acagacacta tttaccgccg ctaccgcggg caacggcgac aacttcacca
    52981 aactggcaga ggcactggcg agcggtgggg cgatggatca cgacaccgct tatgtcgaag
    53041 cggtaggttc tctgcacact ctaggcgggt tgcaatatct tggtctggaa ttaccggaag
    53101 atcgctatgg cgcgattctt cgttatcaaa ccgaccatga cgaagccggg cgtgcaacct
    53161 cgtgtgggcc gcgtacctca aggttaatgg tgaaagtgct gttggaagaa gtacagcgcc
    53221 tcgccattcc agtgttgacc agtgcaacag tgattaaact gctgcatcag cgtgacgaaa
    53281 acggcgaaga ccgcgtggca ggggcaatcc tcgcgaccgg tcatcgcgcc cataacccat
    53341 gggggctggc aattgtgact gcgcccaatg tggtactggc aacgggcgga ccgggcgagc
    53401 tttatcgcga cagtgtttat ccacataaat gcttcggctc gctggggctg gcgctggagg
    53461 aaggtctgac gctaaccaat ctgaccgaaa gccagtttgg tataggcacg ccgcgtagca
    53521 cgtttccgtg gaatttatcc ggcacctatg tgcaggtgat cccgtatatc tattccgtgg
    53581 atgctgaggg taacgagtat aacttcctcg cggattacta tcgcaccacc caggagctgg
    53641 cttcaaacat tttccgtaaa ggctatcagt ggccgttcca cgccactcgg gtgatggatt
    53701 ttggctccag cttgttagat atggcagtag cgcaagagca gcaatcaggg cgtcaggtat
    53761 ttatggattt caatcgcaat cctgaagcgg tgccgggtga cctgccgttc tcattagatc
    53821 gactggacga cgacgttcgc gcgtatctgg aaaataacga cgctctggca ccatcgccca
    53881 tcgaacggct gcaacgaatg aatccgctgt ctatctcgct gtataagatg cacggttacg
    53941 atctcaccac acagcctttg cagtttgcca tgaataatca gcatatgaat ggcggcattg
    54001 aagtggatat ctggggacaa acctccctgc ccggctgttt cgccgtgggg gaagttgctg
    54061 gcacacacgg cgtcactcgc cctggtggtg cagcattgaa tgccgggcag gtttttgctg
    54121 ttcgtctggc acgttttatt ggttgcacgc aaaagcggaa tattgatgga gatatagcac
    54181 agctggtagc tcctacactg gcttctataa gagagatcat tactcaggcg cacgataacg
    54241 ggaccgggat gccgttgtcg gttgtgagag aaaaaattca ggcacgaatg tctgaccatg
    54301 cgggatttat ttgccatgcc gataaagtcc gacgcgccac tcgcgatgcc ctgctattga
    54361 gcgaatttgt ccaacggcat ggattggcta tcaaacatgt gggtgaagtt gccgagctgt
    54421 ttatgtggcg gcatatggca ctgacttctg ccgccgtatt aacgcaatta acgcattata
    54481 ttgacgcagg tggtggtagt cggggagcac gtattattct cgacagggat ggaaattcta
    54541 ttccgcaaac acgaaatggt ttttgtgatg cctggcgctt tcgttcagag cgtactgagg
    54601 ataaaaaaga taaactactt attcattatt gcaacggtat ctttcacgtc agagaaacac
    54661 ctgttcgtga gtttcctatt atccgaggta tttggtttga aaaaaactgg cctggttttc
    54721 ttaatgggac aatttaccag ccacaggatg aatgatattg caaaggttaa tcactatgcg
    54781 aaatataatt agatctatta atgctctact tgcagcattg aacattacca ttttggcgat
    54841 catcgtggca tgtgtgacgt ggcaggtggc tgcacgattt atatttacta gtcccagtat
    54901 ttttacagat gaactcagcc gtttgctctt tatttgtctt ggactttttg gtggggcata
    54961 caccgcaggg caaaaccggc atctggcgat tgacctgcta cctatgatgc tgaaaggaaa
    55021 agcccgacgt catttatttt tgtgtattca gattatcgtt attatcttcg caaccatcat
    55081 tatggtatat ggtggtgggc tattaacgat ggatacattc gattcaggtc agacctcgcc
    55141 cgctttaggc tggcaaatgg gatatatcta tatgagcatc ccgattagtg gtgtacttat
    55201 tattatatac accatagata tggttttaac cgaacttaaa caaccactct aaagataaca
    55261 aacaacaacc aaccatagta ggaatggaga aattatggat tactggttac cgattattgt
    55321 gttatttggt gccttctttt ttatgttggc attaggtgta cctattgttt atgccattgg
    55381 tttatcgaca ctggcatcaa tttccacaca actcgatttc aactcagcat tgagtgtggt
    55441 ttcccaaaag ttagcctccg ggttagatag ttttacgttg ttggcaatcc cattttttat
    55501 cttgtctggc aacattatga accatggagg aatagcgcga cgattaatca actttgcccg
    55561 tattctcggc ggtcgtctgc caggctcact tgcccactgt aatattctcg ccaatatgct
    55621 gttcggggca atttctggtt cagccgtggc ttcggcggct gcgatgggcg gcgtcatgca
    55681 tccgcaacag gttaaagagg gttacgaccc ggcattcagt actgcggtta acgttgcctc
    55741 tgccccgacg ggtttgctta ttccaccaag taatacgtta atcgtctatt cactggtcag
    55801 tggtggtact tctattgccg cactgttcct ggcgggttat gttccgggaa ttttgctggg
    55861 tctggcactg atggtaatag ccggtattat cgcggtacgt cgcggttatc caaagccaga
    55921 acgcccgact ttacgccagg ctggcgttgc gatatggatg gctatcccca gtattttcct
    55981 gattatttta attatggggg gtgtattaag tggtattttt accccgacag aggcctccgc
    56041 aattgcagta atttataccc ttttccttgc actggttctt tatcgtgaaa tctcagttaa
    56101 agatttacct aaaatatttc tggaatccgt tattaccact gcaattgtct tgttattaat
    56161 tggttcttcg atgggtatgt catgggcaat gtcaaacgct gacgttccct tcctgattct
    56221 cgatttgctt aacactattt ctgacaaccc aataataatc ctgcttatta tcaatattat
    56281 cctgttgatt atcggcactt ttatggatat gacaccggcg gtactgattt ttacgccgat
    56341 atttttaccg gttgtcaccg aactgggcat ggacccgatt cactttggta ttgtcatggt
    56401 actgaacatg tgtatcggta tttgtactcc gcctgtaggc agtgtgttat ttgtcggttg
    56461 tagtgtatct aaattaccga ttaataaaat tatcaaacct atgctgccat tctatgccgt
    56521 gatggtattg gtgctggcaa tggtgaccta tattccgcaa atcagtatgg cattgccaag
    56581 agcactgggc tactgatgcg cattcactgg cggcacctgc cgccatattt attagaaaag
    56641 gaacatctat gaaaataaaa gtatccgctg gcattatcgg tgctgttctt atgttatccg
    56701 caagccagtc ctgggcagtg acattaaaac tgagtcataa tcaggataag tctcatcctg
    56761 ttcataaagc gatggagttc tttgcgaaaa agagcaaaga gtactctaac ggtgatatta
    56821 ctattcgtat ttatccaaat ggaacattgg gtactcaacg agaaacaatg gagctgattc
    56881 gttctggcgc tattcccctg gtaaaaacca atgcggcaga aatggaagca tttgaaaatt
    56941 tctataaatt atttagcctg ccttatttgt tccgcgatcg tgatcattat tatcaggtca
    57001 tgcagggcga tatcgggaga aaaatcctcg actcaacgaa aagcaaaggt tatttcgggc
    57061 tgacttttta tgatggaggc gcccgcagtt tctatggcaa taaaccagta ctgaaaccag
    57121 acgatctgaa aggcatgaaa gtccgtgtcc agccaagccc tggcgcagtt gaaatgatca
    57181 aagtcatggg cggtaacccg acgccactgg attacggcga gttgtataca gccttacagc
    57241 agggtgtggt cgatatggca gaaaacagcg tgatggcgct gaccaccatg cgtcacggtg
    57301 aagtggcaaa atccttcagc cttgacgaac acactatggt tcccgatgtg gttctgatga
    57361 gcaatgctgc gtttgataaa cttagcccgg aaaatcaggc agttatatta aaagcagcta
    57421 aagaatcaat gagctatatg aaagacttgt ggagcgagga agagaaacaa gaatttgcga
    57481 aactggataa aatgggcgtg aaagtctacc aggtagataa agctccgttt atcgagaaag
    57541 tacagccaat gtacgcaaac ttcgctaagg acaacccagc ccttgcccca atgctggctg
    57601 atattcaggc agctaagtaa ttatcttcgt agggctgctt attgtggccc ttatcgatat
    57661 ctgcaagtgc tcgctacaaa agcaaaacgc cgaccaatgg tcggcgtttt tacgtctcgt
    57721 tgaataacga attattcagc tactacgttt acgatcactt tcgcgaatac ttcgctgtga
    57781 acctggaagc tcacttcgtg ttcgccagtg gtacgcagaa cgccgttcgg cagacgaact
    57841 tcgctcttag ccacttcaac gccagctgca gttacagcgt cagcgatgtc gcgagtaccg
    57901 atggaaccga acagtttacc ttcgtcgcca gctttagacg cgatggtaac agtttccagt
    57961 gcattgattt tctcagcgcg agcattagca gctgccagaa cttcagccag tttagcttcc
    58021 agttcagcgc gacgtgcttc gaagaattca atgtttttct tggtagctgg aacagcttta
    58081 ccctgcggta ccaggaagtt acgagcatag cccgctttaa cgtttacctg atcacccagg
    58141 ctacccaggt ttgctacttt atcaagcaga ataacttgca ttaccttatc ctctcaaagt
    58201 cgtattaatg gaccgtgacc gattactgat ggcgatcagt gtacggcagc agggacaggt
    58261 agcgagcgcg tttgatagcg cgagccagct gacgctggta ttttgcacgg gtaccggtga
    58321 tacggcttgg gacaatctta ccgctttcgg tgatgtagtt tttcagcgta gcgatatctt
    58381 tatagtcgat ctcttgaacg ccttccgcgg tgaaacggca gaacttgcga cgacggaaat
    58441 aacgtgccat atggctagtc tccagaatct atcaattcaa tctgctcggc atgcaaaacc
    58501 attttgctca gtccgttctt tgccttgtgg catgaaatga acccctgaac ggttatgcga
    58561 ctgccgaccg ttatactgtg agtaatggcc tggttttcgt gtccgctaac aataacgggc
    58621 atttgacacc atgcctgccg gtgaaagccg gcttcctcct gcacagaacg atgctcaagc
    58681 acgaactggc agtgaggaat tcctgatgga ctgacctttc gaaggggagt cctgcacacg
    58741 gtgccggaca acaccagacg gttggtcatc agaaattact cttcagaatc cccagcttca
    58801 gcatcatcag cggtttcgtt tgcgaaatca tcgcgacgct cacggcgctc gtctttcgct
    58861 ttaaccatcg gagatgcttc ggtaacagcg tgcttggtac gcataaccat gctgcggata
    58921 acggcatcgt tgaagcggaa ggtagtttcc agctcatcga tcacttcctg cggagcttca
    58981 acgttcatca gaacgtagtg tgctttgtgc agtttgttga tcgggtaagc cagctgacgg
    59041 cggccccagt cttccagacg gtggatcttg ccttctgcac cagtgatggc agcagtgtag
    59101 cgctcgatca tgcccggaac ctgttcgctc tggtcaggat ggaccataaa aacgatttcg
    59161 taatgacgca tcgaattgct ccttacggat tattcagcct cctgtctggg tcagccgaat
    59221 cccggggagg caaggaacgt gttaaaggtc ggctgaaaaa tgacgcgtta tagtacttgt
    59281 atgccccgct aaactcaagg gcagagccga aataaatctc ccctttcgca attaacctct
    59341 aaaccgctga tttggagttc atcacacttc atcatggtgt ttttttgaac aactgcatca
    59401 gaaatggtca ccatcaactt ctttatcatt gattaaaatt aaatcagaag atcatcacag
    59461 aggagagcac acaaagtcag ccgtcgagga ggtatctatg ttcagtcgag ttttagccct
    59521 tctggctgtg cttttgctaa gtgcaaatac atgggcagcc attgaaatca ataaccacca
    59581 ggccagaaat atggacgata tgcaaagctt aggcgtgatt tatatcaatc ataatttcgc
    59641 cactgaaagt gaagcacgtc aggcattaaa tgaagaaaca gatgcgcagg gcgcaacgta
    59701 ctaccacgta attctgatgc gggaaccggg gagtaacggc aatatgcacg ccagcgcgga
    59761 tatttatcgc tagcaccagg tataaccaac gaaacattgc catagtttgc tttgccccct
    59821 tcgcaggggg ctttttttga aaaacggctg cggaattaga ccagttatct cccgaagaag
    59881 gaaatttccg cagcgcgtgt tttacttctg cccgtaataa gcgttaggac catgcttacg
    59941 catgaagtgt ttattcatca ggaagctgtc gatgtgattg agttgtggat taatactgcg
    60001 cgcaatccac gccattttcg ccacttcttc catcactacc gcgttatgca ccgcgtcgtg
    60061 ggcatctttc ccccaggcga acggtccgtg ctgatacacc acaattcccg gcgtatgcag
    60121 cggctcggcg ttgcccagcg tttcgataat cactttgcca gtgttcagtt catactcacc
    60181 ctgcacctct tcttcgctta atccacgcgt acacgggatg tcgccaaaga agtagtcggc
    60241 atgcgtagtg cctaacgccg ggatcgccag ccccgcctgc gcccatgcgg tagcatgagt
    60301 ggagtgggta tggacaatgc cgccaagcga cggataacga cggtagagtt caagatgcgt
    60361 cgcggtgtcg gaagacggac gatattcccc ttccaccacc ttaccgctca tatcgaccac
    60421 caccatatcg tctgctttca tggtttcgta ggcgacgccg ctgggcttga tcaccaccag
    60481 cccgcgttcg cggtcgatag cgctgacgtt gccccaggta aaggtcacca gcccgtagcg
    60541 cggcagatcc atgttggctt caaatacctg ctgttttagc ttttgcatta tgccgcctcc
    60601 accatgcccg ctttcgccat gcgcgctttc acccaatcac gcgctttcgc cacttctgcc
    60661 gccgggtctt ccgccgtttc gctccacatc tcaatcaggt acggcccgca atagccactc
    60721 tgtttgagcg tttcgaaaca acgttcgaaa tccactacac cttcgccaaa cggcacgttt
    60781 ttgaagacgc caggtttggt gtctttcaca tgtaccgcga cgatatgccc gattccggcc
    60841 tgcaactcca tctgcacatc gttgtcccac gccgacaggt tgccgatatc cgggtagagc
    60901 tggaaccacg ggttgttgag atagtgcgcg taacccagcg ccttgctgat ggagttcatc
    60961 aacggataat ccatgatctc catcgccagc gtcacctgcg cgcggctcgc catctcaacg
    61021 ctctctttca ggccgtcacg gaaacgacga cgcgtttcgt tattggcttc ctgatagtaa
    61081 acgtcatagc ccgccagctg gatcacgcga atgccgacgt cctgggcgaa ctggatagct
    61141 ttacgcataa tctccagccc ctgcgcccgc accgcgtcat cttcactgcc cagcgggaaa
    61201 cgacgatgag cagaaaggca catggacggc acgcgcacgc cggtttcaac aatcgcattc
    61261 accagcgcca gacgctgctc gcggctccag tcgaggcgcg acaggcgctc gtccgtttca
    61321 tctaccgaca tctcgacaaa atcgaagccc aacgtttttg ccagttgcag gcgttccagc
    61381 cagcactccc cggcggggag cgctttttca tagatgccaa gcgggatttg tttggacaac
    61441 atatccgctc cttagcccca cagttcggcg atggaacgtt tgaactgacg cgcggcttca
    61501 accggagaag cggcatcacg gatgctgcga ccggcgataa acacatgaat tggaatgcct
    61561 ttgaacagcg gcagatcttc cagcgccagg ccaccggtaa cggtgacttt gaagcccata
    61621 tcggaaagac gtttgatcgc agtgatatcg gcttcacccc acgccacgcc agcggcctgc
    61681 gcgtcacggc tgcggtgata aaccacctgc tgaatacctg cgtcgcgcca ctgttgcgcc
    61741 tgttcccagg tccagtaacc ggtcagttcg atctgcacgt cgccgttaaa ctcttttgct
    61801 acgtccagcg cacctttggc ggtgttgata tccgcacaac aaatcacggt cacccagtca
    61861 gcgttggctt cgaagcacat acgagaaaga attttgcccg catcggcaat tttggcgtct
    61921 gccagcacga ttttgtgcgg gtagagcgct ttcagatcgc gaaccgcacg cacgccttcg
    61981 cccacgcaca gaatggtacc cacttcgata atgtcgactt cttcggcaat caggcgagtg
    62041 gtttcgtagg cgctatccat agtctggttg tccagcgcga cttgcaacat cggtaatgac
    62101 atcttcaatt ccttcttaag ctgccgcgtt ggtgcggtca atgagatcca gtacttcctg
    62161 ctcggtacgg caggcacgta aacggtcgaa attttcctca tcttcaaaca ggttgacgat
    62221 ctgcatgatg cccacttcct ggtgcgtgtt ggcatcgacc gccgccatgg tgatgaggat
    62281 atccaccggg tcgttatctt cgtggttgaa ctccagcggc tttttcagcg tcaccagcgc
    62341 aaaaccggtt ttcttaacac cctcttccgg gcgaccgtgc ggcattgcca ggccaggtgc
    62401 gagcacaaag tacggaccgt gctgttcaac cgcatccaga atcgcctggt agtaacgcgg
    62461 ctcgacaaca tccgccgcca ccagcaggtc aacgccgatt tttactgcgt cctgccatgt
    62521 ctcagcttca gcctgcaggc ggatggattt attttccgcc agcgaatcac gtaatttcat
    62581 ggcgcgtcct tacttcacat cctgcgggaa atgctctttg atcacttcca gcagtttcgg
    62641 gccaaaatcg gcaggagaga gcatgttgcg cacgccaacc acgtatttgt tgccggtcac
    62701 ggagatctcg cccgcgatgt gcgtggaagc gatgataata tccgcgttgc tcagctcact
    62761 tttgtactcg ccaaccgcgc agctgtttac cgtgtggtca atctttgatt gggttaaaaa
    62821 ctggtccact ttcattttca tgatcatgga actaccttgt ccgttgccac acacagccag
    62881 aatacgtacg gtcataatca aaactcctta ttaagcagac tgttctgcca gttgtttttc
    62941 tgcatcttct tcagcgcgca gtgcgcggcc agcgaagaac atataagcca gtgcaattac
    63001 aatgatgacg gccataaagg cgataccgat ggagaagaag ccctgcatca tcggcggtgc
    63061 cagaatcgac cagtccgcca tgcccatcca ggcgctcata ccggtgagtt tcaccgccca
    63121 gacgcagcca aagatttcaa tcatccccat caccagacat atcttcagcg ccgcacgcca
    63181 gccgccgaag tggttagcga acacgccgat ggtggcgttg gagaagaaca tcgggataaa
    63241 gccgggaata atcaggatcg aggaaccgca ggcgaccagg atgccaaccg caatcagctg
    63301 accgatggtg ccccacataa agccccaaac cacggcgttc ggcgcgaagc tatagatagc
    63361 cgcacagtca atcgccagaa ccgcaccagg gatcaggcgc tgggagatac cgttaaatgc
    63421 ttcagagagt tccgccacaa acatgcgcac gccctgagtg atgatgaaga tcgccaccgc
    63481 gaaggagaaa ccagtttgca ggatgtacac cgtccagttc actttgcctg ccatcgcctg
    63541 cacggtgtcg ataccgaagg agagcagaat ggcaccaaag aagatggtca tcacaatcgc
    63601 cgtggagacg atgttgtcgt ggaaaatatt cagccagcct ggcagtttga ggtcttcaac
    63661 gctttcctct tttttgccga ggaacggcgc aactttatag gcaatccatg atgcaaactg
    63721 ttgctggtga ccgatggaga aaccgcagcc gtccgtcact tcctgagtcg gcttgtacat
    63781 tatgttggag gtaatgcccc agtagagcga aaccagaatc gccgtgcaga taatggtcgt
    63841 ccacatggag tagccgaaaa taaacaacgt aacggcaatc agccccgcct gctggaacat
    63901 gatatgacca gtcagcatga tggtacgaat accggtaatg cgacgcagca gcacgtaaca
    63961 gatgttcaac gccagcgcta acaacaccgc gtaacctacc cagttatagg catcgcccat
    64021 gcggtcgatg gttgccatca tcgacgcgta agtatcggaa attgcgccgt taatgccgta
    64081 gacttcggac attttcgcca ccaccggttt gaaggtgctg gtgaggatgc cggaccctgc
    64141 ctgcaacaac atgaaaccaa ttatggtttt aatcgtgcct ttaataataa cgctgacact
    64201 tttgcgcagt aggatgtagc ccagacaggt cacaataccc agcaacaacg gggcattggt
    64261 catgacctgg ttaaaaaaca cggtaaagat gttgtagagg atctccataa cgatctcctg
    64321 aagaagaggt aaccgggtat tccacacacc cggaatgttg tgcactcact ctaattttca
    64381 aaagtaatca caacaagatt attt
//
LOCUS       DAZFXT010000013        61018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.013, whole genome shotgun sequence.
ACCESSION   DAZFXT010000013 DAZFXT010000000
VERSION     DAZFXT010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 61018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.013"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(15..1151)
                     /gene="hemW"
                     /locus_tag="ACHKW0_000915"
     CDS             complement(15..1151)
                     /gene="hemW"
                     /locus_tag="ACHKW0_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HFO7473796.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEDKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(1144..1737)
                     /gene="rdgB"
                     /locus_tag="ACHKW0_000916"
     CDS             complement(1144..1737)
                     /gene="rdgB"
                     /locus_tag="ACHKW0_000916"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HFO7473797.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(1745..2035)
                     /gene="yggU"
                     /locus_tag="ACHKW0_000917"
     CDS             complement(1745..2035)
                     /gene="yggU"
                     /locus_tag="ACHKW0_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein YggU"
                     /protein_id="HFO7473798.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(2032..2598)
                     /gene="yggT"
                     /locus_tag="ACHKW0_000918"
     CDS             complement(2032..2598)
                     /gene="yggT"
                     /locus_tag="ACHKW0_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HFO7473799.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(2616..3320)
                     /gene="yggS"
                     /locus_tag="ACHKW0_000919"
     CDS             complement(2616..3320)
                     /gene="yggS"
                     /locus_tag="ACHKW0_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HFO7473800.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            3338..4318
                     /gene="yggR"
                     /locus_tag="ACHKW0_000920"
     CDS             3338..4318
                     /gene="yggR"
                     /locus_tag="ACHKW0_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HFO7473801.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSYQQRVGEGRL"
     gene            complement(4507..4923)
                     /gene="ruvX"
                     /locus_tag="ACHKW0_000921"
     CDS             complement(4507..4923)
                     /gene="ruvX"
                     /locus_tag="ACHKW0_000921"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HFO7473802.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(4923..5486)
                     /gene="yqgE"
                     /locus_tag="ACHKW0_000922"
     CDS             complement(4923..5486)
                     /gene="yqgE"
                     /locus_tag="ACHKW0_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HFO7473803.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(5595..6545)
                     /gene="gshB"
                     /locus_tag="ACHKW0_000923"
     CDS             complement(5595..6545)
                     /gene="gshB"
                     /locus_tag="ACHKW0_000923"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HFO7473804.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(6558..7289)
                     /gene="rsmE"
                     /locus_tag="ACHKW0_000924"
     CDS             complement(6558..7289)
                     /gene="rsmE"
                     /locus_tag="ACHKW0_000924"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HFO7473805.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(7369..8076)
                     /gene="endA"
                     /locus_tag="ACHKW0_000925"
     CDS             complement(7369..8076)
                     /gene="endA"
                     /locus_tag="ACHKW0_000925"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HFO7473806.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(8171..8668)
                     /gene="yggI"
                     /locus_tag="ACHKW0_000926"
     CDS             complement(8171..8668)
                     /gene="yggI"
                     /locus_tag="ACHKW0_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HFO7473807.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(8745..10139)
                     /gene="galP"
                     /locus_tag="ACHKW0_000927"
     CDS             complement(8745..10139)
                     /gene="galP"
                     /locus_tag="ACHKW0_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HFO7473808.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            complement(10575..11729)
                     /gene="metK"
                     /locus_tag="ACHKW0_000928"
     CDS             complement(10575..11729)
                     /gene="metK"
                     /locus_tag="ACHKW0_000928"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HFO7473809.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            11785..12036
                     /gene="yqgD"
                     /locus_tag="ACHKW0_000929"
     CDS             11785..12036
                     /gene="yqgD"
                     /locus_tag="ACHKW0_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgD"
                     /protein_id="HFO7473810.1"
                     /translation="MVADPTTTLQVKNTGSLSVNRYGWINIWMAILGQFFTRFPLFFE
                     SCLILLKTWLEIFPDNAGILRIYLLQFSAIVGYKTRRAA"
     gene            12147..12290
                     /gene="yqgG"
                     /locus_tag="ACHKW0_000930"
     CDS             12147..12290
                     /gene="yqgG"
                     /locus_tag="ACHKW0_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgG"
                     /protein_id="HFO7473811.1"
                     /translation="MNRCLLLNLSHRSGEDSFPALCISALHTCRCYTHLGASQDSRAG
                     YSY"
     gene            12384..12515
                     /gene="yqgB"
                     /locus_tag="ACHKW0_000931"
     CDS             12384..12515
                     /gene="yqgB"
                     /locus_tag="ACHKW0_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HFO7473812.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            12524..14500
                     /gene="speA"
                     /locus_tag="ACHKW0_000932"
     CDS             12524..14500
                     /gene="speA"
                     /locus_tag="ACHKW0_000932"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HFO7473813.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            14637..15557
                     /gene="speB"
                     /locus_tag="ACHKW0_000933"
     CDS             14637..15557
                     /gene="speB"
                     /locus_tag="ACHKW0_000933"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HFO7473814.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            15708..17270
                     /locus_tag="ACHKW0_000934"
     CDS             15708..17270
                     /locus_tag="ACHKW0_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7473815.1"
                     /translation="MERTLPWKPLLCLLFLLSTGCDRAEKQKIAAQLQQDKEAISSVV
                     KKSYRALCGPEFVTSTDSHDRLAEMQQVFTPALMEPVLKYWQESPKTCKRRFPSYSDP
                     LATFVEGDKAQIGAFFGHQSYIDIPMELIREGDGKWKIAAIDFASLHEFEKNGRVTDE
                     VQKKIDLLKKRSFKQHRDFPLLTRTAPERVSNSPLTSVEQTVTEPDSSWLDVLLTIGK
                     YALYLVAIILILALVAVLWIGYGLVFAGHRLFKASCKDPSIQKIPAAERWAMAVGAPY
                     AIAGNNHWARRVVNDDSVEAENDCKSEVESLADMWGVFDRDSLLEQLLALFVAGHRSV
                     YAEQIKNDSEMPDAEYRAFAQQLAQNAKSSSEAKERLWQLQMVRKNRRKICDIDFCAW
                     DMVRFVMLCNSGAQVGYITQREMVDFSMLAARRVQQHYRSWRELAGHFLLARWYWKAT
                     DKRHLITHILFKKAITSLLKEQGSPWRTLPWDTPLPEGTETAFAWACGVSTADVVEVQ
                     PDTPDKAIYEAN"
     gene            complement(17356..18114)
                     /gene="loiP"
                     /locus_tag="ACHKW0_000935"
                     /gene_synonym="yggG"
     CDS             complement(17356..18114)
                     /gene="loiP"
                     /locus_tag="ACHKW0_000935"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HFO7473816.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            18392..20383
                     /gene="tkt"
                     /locus_tag="ACHKW0_000936"
     CDS             18392..20383
                     /gene="tkt"
                     /locus_tag="ACHKW0_000936"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7473817.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            20697..21140
                     /gene="cmtB"
                     /locus_tag="ACHKW0_000937"
     CDS             20697..21140
                     /gene="cmtB"
                     /locus_tag="ACHKW0_000937"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HFO7473818.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            21168..22556
                     /gene="cmtA"
                     /locus_tag="ACHKW0_000938"
     CDS             21168..22556
                     /gene="cmtA"
                     /locus_tag="ACHKW0_000938"
                     /EC_number="2.7.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HFO7473819.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNLGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            22571..23848
                     /gene="yggP"
                     /locus_tag="ACHKW0_000939"
     CDS             22571..23848
                     /gene="yggP"
                     /locus_tag="ACHKW0_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HFO7473820.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            23845..24810
                     /gene="glpX"
                     /locus_tag="ACHKW0_000940"
     CDS             23845..24810
                     /gene="glpX"
                     /locus_tag="ACHKW0_000940"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7473821.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDTYMKKLVVNRLAAGAIDLSLPLADNLRNVAKALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            24832..25341
                     /gene="fumE"
                     /locus_tag="ACHKW0_000941"
     CDS             24832..25341
                     /gene="fumE"
                     /locus_tag="ACHKW0_000941"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HFO7473822.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            25338..26051
                     /locus_tag="ACHKW0_000942"
     CDS             25338..26051
                     /locus_tag="ACHKW0_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HFO7473823.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVSPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLCQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(26023..26693)
                     /locus_tag="ACHKW0_000943"
                     /pseudo
     CDS             complement(26023..26693)
                     /locus_tag="ACHKW0_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(26687..27364)
                     /locus_tag="ACHKW0_000944"
     CDS             complement(26687..27364)
                     /locus_tag="ACHKW0_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7473824.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(27352..28059)
                     /locus_tag="ACHKW0_000945"
     CDS             complement(27352..28059)
                     /locus_tag="ACHKW0_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HFO7473825.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAIRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            complement(28060..28638)
                     /locus_tag="ACHKW0_000946"
     CDS             complement(28060..28638)
                     /locus_tag="ACHKW0_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HFO7473826.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(28661..29092)
                     /locus_tag="ACHKW0_000947"
     CDS             complement(28661..29092)
                     /locus_tag="ACHKW0_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HFO7473827.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            29464..30483
                     /gene="epd"
                     /locus_tag="ACHKW0_000948"
     CDS             29464..30483
                     /gene="epd"
                     /locus_tag="ACHKW0_000948"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048001 - erythrose-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HFO7473828.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            30533..31696
                     /gene="pgk"
                     /locus_tag="ACHKW0_000949"
     CDS             30533..31696
                     /gene="pgk"
                     /locus_tag="ACHKW0_000949"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HFO7473829.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            31911..32990
                     /gene="fbaA"
                     /locus_tag="ACHKW0_000950"
     CDS             31911..32990
                     /gene="fbaA"
                     /locus_tag="ACHKW0_000950"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HFO7473830.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(33054..33152)
                     /locus_tag="ACHKW0_000951"
                     /pseudo
     CDS             complement(33054..33152)
                     /locus_tag="ACHKW0_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            33348..34208
                     /gene="mscS"
                     /locus_tag="ACHKW0_000952"
     CDS             33348..34208
                     /gene="mscS"
                     /locus_tag="ACHKW0_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HFO7473831.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            34347..34982
                     /gene="argO"
                     /locus_tag="ACHKW0_000953"
     CDS             34347..34982
                     /gene="argO"
                     /locus_tag="ACHKW0_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0061459 - L-arginine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HFO7473832.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            35075..35815
                     /gene="yggE"
                     /locus_tag="ACHKW0_000954"
     CDS             35075..35815
                     /gene="yggE"
                     /locus_tag="ACHKW0_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HFO7473833.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVSDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            35982..36878
                     /gene="ygfI"
                     /locus_tag="ACHKW0_000955"
     CDS             35982..36878
                     /gene="ygfI"
                     /locus_tag="ACHKW0_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7473834.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMSASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(36875..38353)
                     /gene="scpC"
                     /locus_tag="ACHKW0_000956"
     CDS             complement(36875..38353)
                     /gene="scpC"
                     /locus_tag="ACHKW0_000956"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HFO7473835.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(38377..39162)
                     /gene="scpB"
                     /locus_tag="ACHKW0_000957"
     CDS             complement(38377..39162)
                     /gene="scpB"
                     /locus_tag="ACHKW0_000957"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HFO7473836.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHAIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(39173..40168)
                     /gene="meaB"
                     /locus_tag="ACHKW0_000958"
     CDS             complement(39173..40168)
                     /gene="meaB"
                     /locus_tag="ACHKW0_000958"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HFO7473837.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNTLRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLIVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(40161..42305)
                     /gene="scpA"
                     /locus_tag="ACHKW0_000959"
     CDS             complement(40161..42305)
                     /gene="scpA"
                     /locus_tag="ACHKW0_000959"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HFO7473838.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIIAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRIVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(42509..43402)
                     /gene="argP"
                     /locus_tag="ACHKW0_000960"
     CDS             complement(42509..43402)
                     /gene="argP"
                     /locus_tag="ACHKW0_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HFO7473839.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            43544..43729
                     /locus_tag="ACHKW0_000961"
     CDS             43544..43729
                     /locus_tag="ACHKW0_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473840.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            43785..44444
                     /gene="rpiA"
                     /locus_tag="ACHKW0_000962"
     CDS             43785..44444
                     /gene="rpiA"
                     /locus_tag="ACHKW0_000962"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HFO7473841.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            44700..45932
                     /gene="serA"
                     /locus_tag="ACHKW0_000963"
     CDS             44700..45932
                     /gene="serA"
                     /locus_tag="ACHKW0_000963"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HFO7473842.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            46161..46220
                     /gene="ibsC"
                     /locus_tag="ACHKW0_000964"
     CDS             46161..46220
                     /gene="ibsC"
                     /locus_tag="ACHKW0_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HFO7473843.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            46553..46612
                     /locus_tag="ACHKW0_000965"
     CDS             46553..46612
                     /locus_tag="ACHKW0_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7473844.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(46713..47315)
                     /gene="fau"
                     /locus_tag="ACHKW0_000966"
     CDS             complement(46713..47315)
                     /gene="fau"
                     /locus_tag="ACHKW0_000966"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HFO7473845.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(47336..47519)
                     /gene="ssrS"
                     /locus_tag="ACHKW0_000967"
     ncRNA           complement(47336..47519)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="ACHKW0_000967"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(47561..47890)
                     /gene="zapA"
                     /locus_tag="ACHKW0_000968"
     CDS             complement(47561..47890)
                     /gene="zapA"
                     /locus_tag="ACHKW0_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7473846.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            48058..48636
                     /gene="ygfB"
                     /locus_tag="ACHKW0_000969"
     CDS             48058..48636
                     /gene="ygfB"
                     /locus_tag="ACHKW0_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HFO7473847.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            48662..49987
                     /gene="pepP"
                     /locus_tag="ACHKW0_000970"
     CDS             48662..49987
                     /gene="pepP"
                     /locus_tag="ACHKW0_000970"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HFO7473848.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYTYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            49984..51162
                     /gene="ubiH"
                     /locus_tag="ACHKW0_000971"
                     /gene_synonym="visB"
     CDS             49984..51162
                     /gene="ubiH"
                     /locus_tag="ACHKW0_000971"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HFO7473849.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGETLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            51185..52387
                     /gene="ubiI"
                     /locus_tag="ACHKW0_000972"
                     /gene_synonym="visC"
     CDS             51185..52387
                     /gene="ubiI"
                     /locus_tag="ACHKW0_000972"
                     /gene_synonym="visC"
                     /EC_number="1.14.13.240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HFO7473850.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLVADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(52461..52544)
                     /locus_tag="ACHKW0_000973"
     CDS             complement(52461..52544)
                     /locus_tag="ACHKW0_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473851.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            52834..53928
                     /gene="gcvT"
                     /locus_tag="ACHKW0_000974"
     CDS             52834..53928
                     /gene="gcvT"
                     /locus_tag="ACHKW0_000974"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HFO7473852.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            53952..54341
                     /gene="gcvH"
                     /locus_tag="ACHKW0_000975"
     CDS             53952..54341
                     /gene="gcvH"
                     /locus_tag="ACHKW0_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HFO7473853.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTFTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            54460..57333
                     /gene="gcvP"
                     /locus_tag="ACHKW0_000976"
     CDS             54460..57333
                     /gene="gcvP"
                     /locus_tag="ACHKW0_000976"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HFO7473854.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPIGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFNVLLGDNHGLDID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            57594..58337
                     /gene="ygfF"
                     /locus_tag="ACHKW0_000977"
     CDS             57594..58337
                     /gene="ygfF"
                     /locus_tag="ACHKW0_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7473855.1"
                     /translation="MVIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(58394..59827)
                     /gene="bglA"
                     /locus_tag="ACHKW0_000978"
     CDS             complement(58394..59827)
                     /gene="bglA"
                     /locus_tag="ACHKW0_000978"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HFO7473856.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGSWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            59872..60183
                     /gene="yqfB"
                     /locus_tag="ACHKW0_000979"
     CDS             59872..60183
                     /gene="yqfB"
                     /locus_tag="ACHKW0_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-acetylcytidine aminohydrolase"
                     /protein_id="HFO7473857.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVRLDTLTEKHAEQENMTLTELKKVIADIYPGQTQFYVIEFKC
                     L"
     gene            60347..61006
                     /gene="yqfA"
                     /locus_tag="ACHKW0_000980"
     CDS             60347..61006
                     /gene="yqfA"
                     /locus_tag="ACHKW0_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HFO7473858.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
BASE COUNT        14551 a        15334 c        16318 g        14815 t
ORIGIN      
        1 agacgcatta atgattactc agcgaggaat aattcaagaa gtgagtttaa gaataattta
       61 ccatgttccg ttatctgcca gtaatccgca cattcggtga gataaccctg ggcaatcgcc
      121 tcgtctaact gtgggcgaat cacatcttcg caaagcccgg tatacgcaat aaactccacg
      181 cgcggcgcgg cctccagcag acggaagcga ttcataaaga actcaaacgg cttatctgcg
      241 gcttcgacat cacgctggct ttccagatac cttccttgca taaaaccacg cggatgacgc
      301 gttttagtgg tacgcagaat gcgcccatcc gggaaggtca ctttgccgtg cgcgccgcag
      361 ccaataccga tgtagtcgcc aaagcgccag tagttgagat tgtgctggca ctgataactg
      421 ggtttggcgt aagcggaagt ttcatattgc tgataacccg ctgcggttaa taactgatgc
      481 ccctgttcga atatatccca cagcgcgtcg tcgtccggca gtaccggcgg gcgtgaacca
      541 aacagcgtat taggttcgat ggtcagttga taccaggaaa gatgcggcgg attcagttca
      601 atggcctggc gtagatcgcc aagcgcctct tccagtgatt gatccggtag tccatgcatc
      661 aaatcgaggt taaagctacg cagccccagc ccgctcgcca gctttgccgc gcgtttcgct
      721 tcttgcgggc catgaatacg cccaagtcgt ttcagcttgt cttcgctaaa actctgcaca
      781 ccaatagaga tgcggttcac accagcacgc tgataatcga caaagcgatc ggcttctacc
      841 gtaccagggt tcgcttccat agtaatttct gcatccgctg ccagcggcaa acgcgcacgc
      901 acgccgtcca gcagcgtttg catcgccggg ccggaaagca ggctcggcgt accaccgcca
      961 ataaagattg tctttacttc acggccctga gcgtaagcca catcgttgtc cagatcgcaa
     1021 agcagatgct gaacgtaatc gtcgtgcggc acttctcctt tcaacgcgtg agagttgaaa
     1081 tcgcagtacg ggcatttctg cacgcaccac gggatgtgaa tgtagagact cagcggcggt
     1141 aatttaacca ttacgtaaag cgtccaacag cagtttcaat gcctgaccac ggtgggaaat
     1201 ggcgctcttt tcttcgcggg tcagttcggc agcggttttc ccttcggaag gtacgaagaa
     1261 gattggatca taaccaaagc caccagtgcc cgctggttca cgagtaatca cgcccggcca
     1321 gctaccgtgg cacaccagcg gagtcggatc ttccgcgtga cgcagatata ccagcacgca
     1381 gtggaaacgc gcctgacgtt ggtcgtccgg tacgtctttc agtgtttcca acagtttttg
     1441 cagattcttt tgatcggtcg cgtcttcacc ggaataacgc gcggagtaaa tacccggcgc
     1501 gccacccagc gcatctaccg ccagaccaga gtcgtcggca attgccggta aaccggtcac
     1561 ttttgccgca tggcgcgctt tcagaatcgc gttttcgata aaggtcaggc cggtttcttc
     1621 agcggaatct acgccgaggt ctgtttgggc cacgatatca agaccgaagt cgctaagcag
     1681 cgacgccagc tcacgcactt taccggcatt gccggttgcg aggacaactt tttgcatagg
     1741 atacctaatt aattaacgcc gcgatttctg gcgggatttg ttgcggatta atgattttaa
     1801 tttgtttgtg gcggccaagt tcgccttttt caattaccac ctggcttttg gctacccgga
     1861 attgcttacc gagaaacttc accagatggc tgttggcctg gccgtcaacc ggcggcgcgg
     1921 taatggcgac tttaacttcg tcgccatgta aaccgacaat agaatcacgg ctggctttcg
     1981 gctgaatata gagccgtaaa accagaccgt catcattaac tgttacggca ctcataacgc
     2041 catccacagc cccggcagca gcatgtttcc ggttgcctgt aatacttctg cgacacccat
     2101 attgatgaca tacagcagca gaacgaggat catcggcgag aaatcaatcc cacccattgc
     2161 cggtagcagg cggcgaatcg ggcgcagcag cggatcggcc agctgaatca gcacgtattc
     2221 aatcggacta cgaccctggc ttacccagct cataatcgcc atcaccagca ggacccagaa
     2281 aatcagcagg ccgatggttt tcagcagaat cagtaaaccg gcaatccaga tgattggcag
     2341 gaaggtcacc actttaaaca gcacgatggc tttgataaaa ctgagaatgt acgcaaccag
     2401 cagcgaggcg ctatcaatgg gacccattgc cggaataacg cggcgcagtg gcccgataat
     2461 tggctgtgtt accttcacta caaactgtga gaaggggttg taaaaatcac aatgagccca
     2521 ctgcatccag atgcgtaata acagcaccat ggtatacagc tcaatgaccg ttgaaagcag
     2581 gaaagtcaac gtattcatgg cgttccttta attccttatt ttttagagta atcacgcgca
     2641 ccaaaaattg cagtgccgat acgaaccatc gtgctacctg ccgcaatggc ggcttccata
     2701 tcgtccgaca ttcccagaga gagcgtgtcg atatgcgggt agcgcgtttt cagtccggca
     2761 aatgctacag ccatttggcg tgcaacttca aactgcctta catattctga ctcaggcgca
     2821 gggattgcca tcagcccgcg cagacgtaaa cgcggtagtt cagcgaccgc cgctgccagc
     2881 tcgtccagtt ctgccagttg aatcccggac ttactgtttt catcactaat attaatttga
     2941 atcagtacgt taagaggggg aagttctgcc gggcgctgat cgttgaggcg ggtagcaatg
     3001 cgcaaacggt cgatggtatg acaccagtcg aaatgctctg ccaccaggcg gcttttatta
     3061 gactgcaacg ggccaataaa atgccattct aatcctgtta cgcccagttc ctgaaagtgg
     3121 cgaattttat ctaccccttc ctgaacgtag ttttcaccaa attgacgctg cccggcatca
     3181 atggcttctg cgatggcgct cgcaggtttt gttttactga ctgcaagcag cgtaatttct
     3241 tctggagaac ggccgcaacg cgttgcagcc gctgagattt tgtcccggac ctgtgccagg
     3301 ttatgcgcaa tatcgttcat tttccgagga tcttagtatg aatatggaag aaattgtggc
     3361 ccttagtgta aagcataacg tctcggatct acacctgtgc agcgcctggc ccgcacgatg
     3421 gcgcattcgt ggcagaatgg aagctgcgcc gtttgatgcg ccggacgtcg aagagctact
     3481 gcgggagtgg ctggatgacg atcagcgggc aatattgctg gaaaatggcc agctggattt
     3541 tgccgtgtcg ctggcggaaa accagcggtt gcgtggcagt gcgttcgcgc aacggcaagg
     3601 tatttctctg gcattacggt tgttaccttc gcactgtcca cagctcgaac agcttggcgc
     3661 accaacggta ttgccggaat tactcaagag cgagaatggc ctgattctgg tgacgggggc
     3721 gacggggagc ggcaaatcta ccacgctggc ggcgatggtt ggctatctca atcaacatgc
     3781 cgatgcgcat attctgacgc tggaagatcc tgttgaatat atctatgcca gccagcgatg
     3841 tttgatccag cagcgggaaa ttggtttgca ctgtatgacg ttcgcatcgg gattgcgggc
     3901 cgcattgcgg gaagatcctg atgtgatttt gctcggagag ctgcgtgaca gcgagacaat
     3961 ccgtctggca ctgacggcgg cagaaaccgg gcatttggtg ctggcaacat tacatacgcg
     4021 tggtgccgcg caggcagttg agcgactggt ggattcattt ccggcgcagg aaaaagatcc
     4081 cgtacgtaat caactggcag gtagtttacg ggccgtgttg tcacaaaagc tggaagtgga
     4141 taaacaggaa ggacgcgtgg cgctgtttga attattgatt aacactcccg cggtggggaa
     4201 tttgattcgc gaagggaaaa cccaccagtt accgcatgtt attcaaaccg ggcagcaggt
     4261 ggggatgata acgtttcagc agagttatca gcagcgggtg ggggaagggc gtttgtgacg
     4321 ttgccatgcc cgatgcgcaa gtttataggg cctataaagg caataaatgt ctgatgcgac
     4381 gctaacgcgt cttatcagac ctacaatcgc tgccacatgt aggccggata aggcgttcac
     4441 gccgcatccg gcaataaggc gtaaggcgcg atgatattat acactccagc aggcgtttaa
     4501 atcgccttaa tatccctgct cgaaatagct ttcgagaata ataaccgcag aggcagagtc
     4561 aactttgcct ttgttgagcg cccgatagcc gccctgttca aacagaccgg aacgggcttc
     4621 cacggtgcta agacgttcgt catggagctt tacttcaaca ccgaaacgac catgaatacg
     4681 gttggcaaat ttacgcgctc tggcagtcaa tggttgctcg gtgccgtcca tattcagcgg
     4741 caaaccgacg atgatttcgt ccggctgcca ctctttcagt aaacgttcga tgaggttcca
     4801 gtccggcgta ccgtcctgtg ctttaattgc aggcaaaggg cgagcagtgc cggtaatgcg
     4861 ttggccgacc gctacgccaa tgcttttggt gccgaagtcg aaggcgagta aggttccact
     4921 catcaggcgt gccctgccac accaggcatg gtgagaatat ccacaccaat cagttttgcc
     4981 gcctcgcgcc agcggtcggc aatcggtgtt ttgaacagaa tattcagatc cgccggggcc
     5041 gttagccacg cgttatcgag aatttcttgt tccagttgac ctttttccca ggaggcataa
     5101 cccagagcca ccaatacgtc agacggttgt ttatcggtgc cgagcgtttc cagcacatcg
     5161 cgggaagtgg tcatcactgt gttgtctgaa atgcgaatgc tggaagcaaa attggagggc
     5221 ggagtatgca aaataaaccc gcgatcttca gccagcggac cgccgagcat aaccggttta
     5281 tccagacgga ttgattcatc acgcggctcc ggcgtgatct tcagcttttc cagaatccct
     5341 tcaattttga gattttccag cggcttgttg acgatgatcc ccattgcacc attggtatta
     5401 tgttcgcaaa tgtagaccac ggaacgacgg aaaatcggat cctggagagc aggcatggca
     5461 ataagaaagt gatgctgtaa attcattgtc agaggttctg tttcctggtt caaaaaagca
     5521 acagcgccca gtatgcgtgg aaagaagggc gctgtcactc agagtctcaa cgagatcctt
     5581 ctcgctaagg tgggttactg ctgctgtaaa cgtgcttcga tggcatccat taacattccg
     5641 gtgatcgaca ccggaaactc tgcttcaatc tcacgaatac aggttgggct ggtgacgtta
     5701 atttcagtca gacggtcgcc gatgatatcc agaccaacaa aaatcagccc tttttctttc
     5761 agcgtcggcc cgatctgacg ggcgattttc cagtcacttt ccgtcagcgg acgaggttca
     5821 ccgcgaccac cggcagccag attgccacgg gtttcgcccc cctgcggaat acgcgccagg
     5881 cagtacggta ccggctcgcc atccaccacc agcacgcgtt tgtcgccatc tttaatggct
     5941 ggcaggtaat tttgcgccat gcagtagcga gtgccatgct cagtcagggt ttcggcaatc
     6001 acgccgaggt ttggatcgcc ttctttcacg cggaaaatcg acgcgccgcc cataccgtcc
     6061 agcggcttaa gaatgatgtc gctgtgtttc tcccagaacg cttttagctg cgctttattg
     6121 cgcgtaacca gcgtttctgg cgttaagtca gagaaccagg cggtaaacag tttctcgtta
     6181 cagtcgcgca ggctctgcgg cttgttaacg atcagcgtcc ctttctcttc ggcacgttcc
     6241 agaatatagg tcgcgtagat aaactcggta tcaaacggcg ggtctttacg catcaggatc
     6301 acatcgagat cggccagcgg cagatcctgt tcaccgacga atgaaaacca ctcttcgtag
     6361 ttctgcttca cgttcagcgt gcgggtatgg gcgcgggctt cgccattgat cagatacaga
     6421 tcgcccatct ccatatagtg aagttcgtaa ccacgacgct gtgcttccag caacatagcg
     6481 aaactggaat ctttcttgat gttgatgttt gcgatggggt ccatcacgat gccgagcttg
     6541 atcattattc ttctccgtta gcccaaatcg ccaaatcgta cttgtagcgc ggtaatggcg
     6601 gtgagcgcag ttgtctctgt acgcaaaacg cgaggtccca acaggatatc agtaaattga
     6661 tagcgggcag tcatggcaat ttcatctgcc gataaaccgc cttccgggcc aatcagcagg
     6721 cggacgcgtt caaccggtaa aggcaacgta ttgatgctgt tactggcgcg cgggtgaaga
     6781 ttcagtttca gtccttcatc ctgctctgca caccaggctt ccagatccat cgctggacgg
     6841 atttccggca cccggttacg accacactgc tcacaggcag caattgcaat cttctgccac
     6901 tgctgaagct tcttgttcag acgttcacta tccagtttaa cgccgcagcg ctcagaaaaa
     6961 agtggcgtaa tgaggcttac accgagttcg atcgatttct ggatcgtaaa ttccattttt
     7021 tcaccacgcg acatcacctg accgaggtga atatgcagcg gagattcgcg atcgtcgatc
     7081 ttgccttcca gcaccttcac ttccacgctt tttttgctgg cgctggtaat ttcggcgtca
     7141 aagacctggt tgctaccgtc aaacaattgc aacgcctgcc ccggccccat gcgcagtacg
     7201 cgcccgatat ggttggcggc atcttcgcaa agcgcgatgt gagaatggct ggtcagtggt
     7261 tcaggatgat aaatgcgggg gatacgcata gttaaaaatc cgcgtcgtct ccccacgcgg
     7321 ttgtacgcgt ggggtagggg ttaacaaaaa gaatcccgct agtgtaggtt agctctttcg
     7381 cgcctggcaa gcgcgttgca catacgggtt atgattgccc tgcaccttcg cgatgcgttc
     7441 atcgcgctcg cactcccagt cggtaaccgg atacatcttg ttccatgcgt tgaacagctg
     7501 cgtttgctgg cgagagagtg tcaggttgta ttggtcgcgc atatagaagt aggtgcgcgc
     7561 aatggcaccg cgtgcacgcg ctggtggttc ggcagctttt tctttgaaat cgaccttcat
     7621 ggcgcattga ccgtactggc cttcgccgcc attccactgg ctgtacataa agttgccgcg
     7681 atcgccattc acctcgccga ctgacggttg caggttatgc atatcgcttt ccatcttgcg
     7741 atagaccgga tctttagcgc agtttttacg tccaccgtcc tgccagcact ggcgctggtg
     7801 accgaactgc caggcgggaa cgacgtgttc ccactctacg cggctggcgc ggttttcatt
     7861 tttgcgcacc tgatagccgc acgattgcag atcaacaacg ccttttttgc cctgccagtt
     7921 aattttacat ccgcaataaa acgtaccggg cgcgtcagcg tggactttta ccgccgcggc
     7981 tttcgcctga gaaaaactat tgataccttc ggccaaagcc gggccggaaa atgctgcgct
     8041 cagtaccacc gcagcaatag acaaataacg gtacatcact tactccgtgt taaaacgagc
     8101 cagcaacgta gcgaaagttg ttttggtatg caatcagcca gatcaggaaa gttcctgata
     8161 gttcagatgg ttatttcgca accagctgtt caccgcagtg aacacagcga tagacggcct
     8221 cgccacgaac tacgcgatta tggcggcgta cggtaagctg atgctcctgg cacttgcagc
     8281 ggtaggggaa ggtgttgcga cgcacggatt gcagttcgaa ctgatgcgta cgacgggcgg
     8341 gaacacccag cacgctttcc atcatccact tccactcttt gccatgtggc gctacgcggc
     8401 cgaaatgttt ccataccagc aaatgtgcca gttcgtgcgg taccacttct tcaataaaag
     8461 cttcactgtt ttccaacagc aaaacgggat tgaggcgaat ttcatagctt tccagccagg
     8521 ccgttccggc ggaggttccg cgctgggtgt aagagagttt tggctccggg tagttacgcc
     8581 ctagcttcag gttggcctgg gcgagttttt cccgcaggcg acgcataacg gcctgttgga
     8641 tggcgatagg gagacgggat gttttcatag cggcagagga tagagcaaag aaaagatgac
     8701 tgcaagaggt ggcttcctcc gcgacgggag gaagcctgag gagattaatc gtgagcgccg
     8761 atttcgcgca gtttacgacc tttcatcaga ttacgttcaa tatgttccag cgaaacgtgt
     8821 ttggtttccg gtaccagcca caatgtcagc aggataaaca gtacgttcag agccgcatac
     8881 acccagaagg tgttggcgtt acccagcgtg ttgagcatgg tcaggaacgt tgcgccaacg
     8941 atcatgttgg caatccagtt ggtggcagtg gagcaggtga tgccaaaatc gcggcctttc
     9001 agcggctgaa tttcggagca cagtacccaa atcagcggac cggcactcat ggcaaaaccg
     9061 acaataaaca tcagcagcat ggcgatggcg aaatactgcg ccgacggaga gtgaataccg
     9121 atatgcatca ttgtaccgag tacgcccatg ccagcagcca tcaccaggaa gcccagcgtt
     9181 agcgttggtt tacgtcccca gcggtcaaca aggccgattg cgataaaggt ggcaagtacg
     9241 ttggtcaggc cgacaatcac ggtcccccac atttgctcgg tagtgttggt ataacccgcc
     9301 agttcgaaga ttttcggcgc gtaatacatg atgacgttca tcccggtgaa ttgctgcatt
     9361 acctgcaaca gtacgccaag gaacaccgcg cggcggaagt tgctgttctc tttaaacagc
     9421 gcccagccac tctgtttaac ctgcaaactt tcacggattt catccagttc gcgtttcgct
     9481 tccgcgctgg tgtcacgcag gcgtagcagc acgcgttcgg catcaacaaa acggcgtttg
     9541 gcggcaaacc aacgtgggct gtctggcagg aagaagacac caatcagcag caaaattgcc
     9601 gggatgataa tcacacccag catccagcgc catgcaccgg tgtagctgaa ggcggtatca
     9661 gaaagataag caccgaggat cccgatagtg atcatcaact gatacatcga gatcatactg
     9721 ccacgaattt tttccggcgc aatttcagag aggtacagcg gtgcggtata agaggccaca
     9781 cccaccgcca gccccagtag aacgcgggaa agaatcagta cttcaacgtt tggcgcagcc
     9841 gcagagaaca gcgaaccggc aacaaacaaa attgcgccga tcatcaggct ctttttgcgc
     9901 ccgagtttaa aggagagcca gccgctgccc accgcaccga ctgccgcacc gaacatcatg
     9961 gagcttacga cccattcttg cgtgtgcgaa gtaatctgga attcatctgc aataaacggc
    10021 agtgcgccag caattacacc gatatccagg ccaaagagta atcccgccag agcggcaagg
    10081 aagcagacga aaaacgtcat tgccttgttt gaccgcccct gttttttagc gtcaggcatg
    10141 atgccctcca atatggttat tttttattgt gaattaagat aggtgagtac gacgtaaaaa
    10201 gatgtgaagc aaatcacatc agtgtaatcg cttacactaa catttatctt agttatggtt
    10261 taagtgcatg aaaataattg agttatatca tcatagatta aacgctgtta tctgcaatta
    10321 agactttact gaaaagaaat gtaacaactg tgaaaactga ataataatat gaaaatatta
    10381 caaatatttg gctggtgtta ttggtgtaat cggtttcagt agtgatgata atttgcgata
    10441 aaacgtgttg aggaagaatg atgttattgt gcgggcagct ttttgccgga tgcggcgtga
    10501 atgcctatcc accgcggtga ggtaaaagaa aaaggccagc gcgaggctgg cctttgaaag
    10561 caggtgaaga aagattactt cagaccggca gcatcgcgca gcagctgcgc tttgtcggtt
    10621 ttttcccacg ggaaatgttc acgaccaaag tgaccgtatg ctgcggtttc tttgtagatc
    10681 gggtgcagca gatccagcat ctgaatcaga ccgtatgggc gcaggtcgaa gaactcacgt
    10741 accagcagag tcagttgttc agaaggcact ttctcagtac cgaaagtttc taccatgatg
    10801 gaggtcggtt cagccacgcc gattgcgtag gaaacctgaa tttcacaacg atcggccagg
    10861 ccagcagcaa cgatgttttt cgcgacataa cgtgctgcgt aggctgcgga acggtccact
    10921 tttgatggat ctttaccaga gaatgctccg ccaccgtgac gtgccatgcc gccgtaggta
    10981 tcaacgataa ttttacgacc agtcagaccg cagtcaccca tcgggccacc gataacaaaa
    11041 cgaccggtcg ggttgatgaa gaatttggtg gcagaagtca gccattcagc gggcagaatt
    11101 ggcttgatga tctcttccat taccgcttct tgcagcgatt tctggtcgat ctcttcagag
    11161 tgctgagtgg aaagcacgac agcatcaata ccaacgattt tgccgtcgtc atactggaaa
    11221 gtcacctggc ttttcgcgtc cgggcgcagc cacggcagag tgccgttttt acgcacttca
    11281 gcctgacgct gtaccagacg gtgtgcatag gtgataggtg ctggcatcag cacgtcggtt
    11341 tcattagttg cgtagccaaa catcagaccc tggtcacccg cgccctgttc cagcggatcg
    11401 gcacggtcaa cgccctggtt gatgtcagga gactgtttgc cgatagcgct cagaaccgca
    11461 caggagttag cgtcaaagcc catgtcggaa tgcacatagc caatttcgcg aacggtgtta
    11521 cgggtgatct cttcgatgtc tacccaggcg ctggtggtga tttcgccgcc aactaaaacc
    11581 atgccggttt ttacgtaggt ttcgcaagca acgcgtgctt tcggatcctg ttcgaggatc
    11641 gcgtctaaaa cggcatcaga gatttggtca gcaattttgt caggatgccc ttcagagacg
    11701 gactcggacg taaaaaggtg ttttgccata tttaatatca cctaaagaga atttggttag
    11761 ctcaaactgt tgtgtggatt ttctgtggta gcggatccta ccacgactct gcaagttaaa
    11821 aacactggca gtctgagtgt taatcggtat ggatggatta acatctggat ggctatttta
    11881 ggtcaattct tcacccgatt tccacttttt tttgaatcgt gtctcattct gttaaaaacg
    11941 tggctggaaa tttttcctga caatgccggc attctgcgta tttatctttt gcaattttct
    12001 gccattgtgg ggtataaaac gcggcgcgcg gcttaaataa aaagcacacg acgtttcttt
    12061 cgtgttgcca cttccagccg ggttgaaatc agagttttgg cttgtgggtt cgtcttaaca
    12121 ggcggccgtg gaggtgatac gaaataatga accgttgtct gctgcttaac ctgtctcacc
    12181 gttctggtga agattcgttc cccgcactct gcatctctgc tttgcatacc tgccgatgtt
    12241 atacccatct cggcgcttct caggattcaa gagctggtta cagttactga ggactgaaca
    12301 agggcgctct tgtaaaaaca agagttttct cgtggtttcg ccgaactgtc acacttacgt
    12361 tcggttatgt gcttaataat gttatgaaaa agaaaccggt tgcgcagttg gagcgtcagc
    12421 attcactgct ggaaaatcca tgtgcttatg ggttgttatc gcagttccag gctgcgatag
    12481 tcgttaactg ttttacactt aataaaataa tttgaggttc gctatgtctg acgacatgtc
    12541 tatgggtttg ccttcgtcag cgggcgaaca cggtgtacta cgctccatgc aggaggttgc
    12601 aatgagctcc caggaagcca gcaagatgct gcgtacttac aatattgcct ggtggggcaa
    12661 taactactat gacgttaacg agctgggcca catcagcgtg tgcccggacc cggacgtccc
    12721 ggaagctcgc gtcgatctcg cgcagttagt gaaaactcgt gaagcacagg gtcagcgtct
    12781 gcctgcactg ttctgtttcc cacagatcct gcagcaccgt ttgcgttcca ttaacgccgc
    12841 gttcaaacgt gcgagggaat cctacggcta taacggcgat tacttccttg tttatccgat
    12901 caaagttaac cagcaccgtc gcgtgattga gtccctaatt cattcgggcg aaccgctggg
    12961 tctggaagcc ggttccaaag ccgaattgat ggcagtgctg gcacatgctg gcatgacccg
    13021 tagcgtcatc gtctgcaacg gttataaaga ccgcgaatat atccgcctgg cattaattgg
    13081 cgagaagatg gggcacaagg tctatctggt cattgagaag atgtcagaaa tcgccattgt
    13141 gctggatgaa gcagaacgtc tgaatgtcgt tcctcgtctg ggcgtgcgtg cacgtctggc
    13201 ttcgcagggc tcgggtaaat ggcagtcctc cggcggggaa aaatcgaagt tcggcctggc
    13261 tgcgactcag gtactgcaac tggttgaaac cctgcgtgaa gccgggcgtc tcgacagcct
    13321 gcaactactg cacttccacc tcggttcgca gatggcgaat attcgcgata tcgccacagg
    13381 cgttcgtgaa tcggcgcgtt tctatgtgga actgcacaag ctgggcgtca atattcagtg
    13441 cttcgacgtc ggcggcggtc tgggcgtgga ctatgaaggc acccgttcgc agtctgactg
    13501 ctccgtaaac tacggcctca atgaatatgc caacaacatc atctgggcga ttggtgatgc
    13561 gtgtgaagaa aacggtctgc cgcatccgac ggtaatcacc gaatcgggtc gtgcggtgac
    13621 tgcgcatcac accgtgctgg tgtctaatat catcggcgtg gaacgtaacg aatacacggt
    13681 gccgaccgcg cctgcagaag atgcgccgcg cgcgctgcaa agcatgtggg aaacctggca
    13741 ggagatgcac gaaccgggaa ctcgccgttc tctgcgtgaa tggttacacg acagtcagat
    13801 ggatctgcac gacattcata tcggctactc ttccggcacc tttagcctgc aagaacgtgc
    13861 atgggcagaa caactctatc tgagcatgtg ccatgaagtg cagaagcaac tggatccgca
    13921 aaaccgtgcg catcgtccga taatcgacga actgcaggaa cgtatggcgg acaaaatgta
    13981 cgtcaatttc tcgctgttcc agtcaatgcc ggacgcatgg gggatcgacc agttgttccc
    14041 ggttctgccg ctggaagggc tggatcaagt gccggaacgt cgcgctgtgc tgctggatat
    14101 tacctgtgac tctgacggtg ctatcgacca ctatattgat ggtgatggta ttgccacgac
    14161 aatgccaatg ccggagtacg atccagagaa tccgccgatg ctcggtttct ttatggtcgg
    14221 cgcatatcag gagatcctcg gcaacatgca caacctgttc ggtgataccg aagcggttga
    14281 cgtgttcgtc ttccctgacg gtagtgtaga agtggaactg tctgacgaag gcgataccgt
    14341 tgcggatatg ctgcaatatg tgcagctcga tccgaaaacg ctgttaactc agttccgcga
    14401 tcaagtgaag aaaaccgatc ttgatgctga actgcaacaa cagttccttg aagagttcga
    14461 ggcaggtttg tacggttata cttatctgga agatgagtaa gtccgtatta cttgaatccg
    14521 cttaatttag cggtgataat ccgccacaat ttattgtgac aaatccaacc cttcctcgtc
    14581 gggcctaacg acgcggaagg gtttttttat atcgactttg taataggagt ccatccatga
    14641 gcaccttagg tcatcaatac gataactcac tggtttccaa cgcctttggt tttttacgcc
    14701 tgccgatgaa cttccagccg tatgacagcg atgctgactg ggtgattact ggtgtgccgt
    14761 tcgatatggc cacttctggt cgtgcggggg gacgccacgg tccggcagcg atccgtcagg
    14821 tttcgacgaa tcttgcctgg gaacacaatc gcttcccgtg gaatttcgac atgcgtgagc
    14881 gcctgaacgt cgtggactgc ggcgatctgg tatatgcctt tggcgatgcc cgtgagatga
    14941 gcgaaaaact gcaggcacac gccgagaagc tgctggctgc cggtaagcgt atgctctctt
    15001 tcggtggtga ccactttgtt acgctgccgc tgctgcgtgc tcatgcgaag catttcggca
    15061 aaatggcgct ggtacacttt gacgcccaca ccgataccta cgcgaacggt tgtgaatttg
    15121 accacggcac catgttctat accgcgccga aagaaggtct gatcgacccg aatcattccg
    15181 tgcagattgg tattcgtacc gagtttgata aagacaacgg ttttaccgtg ctggacgcct
    15241 gccaggtgaa cgatcgcagc gtggatgacg ttatcgccca agtgaaacag attgtgggtg
    15301 atatgccggt ttacctgacc tttgatatcg actgcctgga tcctgctttc gcgccgggaa
    15361 ccggtacgcc agtgattggc ggcctgacct ccgatcgcgc cattaaactg gtacgcggcc
    15421 tgaaagatct caacatcgtt gggatggacg tagtggaagt ggctccggca tacgatcagt
    15481 cggaaatcac tgctctggca gcggcaacgc tggcgctgga aatgctgtat attcaggcgg
    15541 cgaaaaaggg cgagtaagta acctgcctgg tcgaattttt tgcacagtcc cttgtctggc
    15601 acaagggact tatcatttaa aacctatcct gttaattaag tcatctttac cctgactgct
    15661 gtttttttaa tggattgtta ttaccgtgtt tttaatggaa taaatagatg gaacgcactc
    15721 ttccctggaa gcctcttctt tgtttgttat ttcttttatc aactggctgc gatagagcag
    15781 aaaaacagaa aattgccgca cagctgcaac aagacaaaga agctatttcc tctgtcgtca
    15841 aaaagagtta tcgcgcatta tgtggccctg agtttgtcac cagcacagat tcgcacgatc
    15901 gccttgcgga aatgcagcaa gtatttacgc ctgctttaat ggagcctgta ctgaaatact
    15961 ggcaagagtc acccaaaacg tgtaaacgac gtttccccag ttatagcgat cctttagcga
    16021 ccttcgtcga aggcgacaag gcgcagattg gtgctttttt tggtcaccaa tcttatattg
    16081 atattccaat ggaattaatc cgtgaagggg atggtaaatg gaagattgcg gcgattgatt
    16141 ttgcatcgtt acatgagttt gaaaaaaacg gtcgggttac tgatgaggtg cagaaaaaaa
    16201 tagatttgct taaaaaacgc tcattcaagc aacataggga tttccctctg ttgacgagga
    16261 ccgcgccaga gcgggtcagt aattcccctt tgacatcggt ggagcaaaca gttacggagc
    16321 cagactcttc ctggctggat gtcttactga caataggcaa atatgccctt tatctggtag
    16381 cgataatact tattctcgcc ttggtggctg ttttgtggat tggttatggc ctggtgttcg
    16441 ccggacatcg ccttttcaaa gcatcctgta aagatccctc aattcaaaaa attcctgctg
    16501 ccgaacgttg ggcaatggca gtcggcgcgc cttatgccat tgcgggtaac aatcactggg
    16561 cgcgacgagt cgttaatgat gattccgtgg aagcagagaa tgattgcaaa agtgaagttg
    16621 agtcacttgc tgacatgtgg ggcgtctttg atcgggattc gttacttgag caactcttag
    16681 ctttgtttgt agcagggcac cgttcagttt atgcagaaca gatcaaaaac gatagcgaaa
    16741 tgccggatgc tgagtatcgc gcgttcgctc agcaattagc tcagaacgcg aaatcaagta
    16801 gcgaagcgaa agagcgtttg tggcagctac agatggtcag aaaaaatcga cgtaaaatat
    16861 gcgatataga tttttgtgcc tgggatatgg tgcgttttgt catgctatgt aatagcgggg
    16921 cgcaagtggg atacattacc cagcgtgaaa tggtcgattt ttcgatgctg gcagccagac
    16981 gcgtacagca acattacaga agctggcgcg aattggccgg gcattttttg ctggcccgtt
    17041 ggtactggaa agcaacggat aaacggcacc tgattaccca tattcttttt aagaaagcca
    17101 ttacatcttt attaaaagag caggggagtc cgtggcgtac tttaccctgg gatactccat
    17161 tacctgaagg aacagagacg gcttttgcct gggcttgtgg cgtaagcact gccgatgtag
    17221 tagaggtgca accggataca ccagataaag caatctacga agcgaattga tgccccaaat
    17281 gattgagctt gtttaatgac tctggccggc tctgaatgaa aatttctccg acattcctgt
    17341 cagagaaata tgaaatcact ttatcccatc cgcgctcatg cgatcgcgaa tatgctgggc
    17401 gcgttcggcg gatgccggat ggtcgtcaaa cattgagctt tggcggcctt cttccagttt
    17461 cgccagtttt tcaaagctgg tggcaagacc tgccgggctg atgccgcgtt ggcgtagaag
    17521 atcgtaagaa taatcatcgg cttctgcttc ctggcgctgg gagaattgcg aattgactaa
    17581 tttctcgccc agatcaccaa gttgtgattg agataaactg ccgacaatcc cgcccgcaga
    17641 ggccgcagct actcgcacgg catttgtacc aagcgccacc tgcatccctt tcttcacatg
    17701 gcctaacgcc acgtgcccca tctcgtgacc gatcaccgct tcgacttcgt tatccgtcat
    17761 catatccatc agcccgctat agacgcggat acagccgtta gccattgcaa aggcgttcac
    17821 atccttcgcc atatacactt tgtaattcac tggctgaccg ttgatattgt tgcctagcgc
    17881 attggcaata gttgtcagac gtttagcgta ttcgctattg gctggcgcaa tcgtcgcctt
    17941 gctgtccatc tcctgacatg cctgatcgct cagggttttc acctgcgcat cactcaaact
    18001 gtaagcctga aaagcttccg ctcctgatga gagcagtccg ttggagtcca tattctggca
    18061 accggtcagt accgttgcca cgctcattgc taccaataag gcgcgaattt tcatgttttt
    18121 tcttccgcac aaggtcagga ttattctaaa aatgcgccgt ttgcaggtga accgacgctc
    18181 agtctcagta taaggaaaag cgcagcggac gggcgagtag attgcgcaac atgcgagcat
    18241 gatccagaga tttctgaagc ggcaaaagga tgttccatgt acatgacgcg cggcttgcgg
    18301 taaattgttg gcaaattttc cggcgtagcc caaaacgcgc tgtcgtcaag tcgttaaggg
    18361 cgtgcccttc atcatccgat ctggagtcaa aatgtcctca cgtaaagagc ttgccaatgc
    18421 tattcgtgcg ctgagcatgg acgcagtaca gaaagccaaa tccggtcacc cgggtgcccc
    18481 tatgggtatg gctgacattg ccgaagtcct gtggcgtgat ttcctgaaac acaacccgca
    18541 gaatccgtcc tgggctgacc gtgaccgctt cgtgctgtcc aacggccacg gctccatgct
    18601 gatctacagc ctgctgcacc tcaccggtta cgatctgccg atggaagaac tgaaaaactt
    18661 ccgtcagctg cactctaaaa ctccgggcca cccggaagta ggttataccg ctggtgtgga
    18721 aaccaccacc ggtccgctgg gtcagggtat tgccaacgca gtcggtatgg cgattgcaga
    18781 aaaaacgctg gcggcgcagt ttaaccgtcc gggccacgac attgtcgacc actacaccta
    18841 cgccttcatg ggcgacggct gcatgatgga aggcatctcc cacgaagttt gctctctggc
    18901 gggtacgctg aagctgggta aactgattgc gttctacgat gacaacggta tctctattga
    18961 tggtcacgtt gaaggctggt tcaccgacga caccgcaatg cgtttcgaag cttacggctg
    19021 gcacgttatt cgcgacatcg acggtcatga cgcagcatcc atcaaacgcg cagtagaaga
    19081 agcgcgcgca gtgactgaca aaccgtccct gctgatgtgc aaaaccatca tcggtttcgg
    19141 ttccccgaac aaagccggta ctcacgactc ccacggtgcg ccgctgggtg atgcagaaat
    19201 cgctctgacc cgcgaacagc tgggctggaa atacgcaccg ttcgaaatcc cgtctgaaat
    19261 ctatgcgcag tgggatgcga aagaagcagg ccaggcgaaa gaatctgcat ggaatgagaa
    19321 gtttgcggct tacgcgaaag cttatccgca ggaagcggct gaatttaccc gccgtatgaa
    19381 aggcgaaatg ccgtctgact tcgacgccaa agcgaaagag tttatcgcta aactgcaggc
    19441 taatccggcg aaaatcgcca gccgtaaagc gtctcagaat gctattgaag cgttcggccc
    19501 gctgttgccg gaattcctcg gcggctccgc tgacctggca ccgtctaacc tgaccctgtg
    19561 gtctggttct aaagcaatca acgaagatgc tgcgggtaac tacatccact acggtgttcg
    19621 cgagttcggt atgaccgcga ttgctaacgg tatctccctg cacggtggtt tcctgccgta
    19681 cacctccacc ttcctgatgt tcgtggaata cgcacgtaac gccgtacgta tggctgcgct
    19741 gatgaaacag cgtcaggtga tggtttacac ccacgactcc atcggtctgg gcgaagacgg
    19801 cccgactcac cagccggttg agcaggtcgc ttctctgcgc gtaaccccga acatgtctac
    19861 atggcgtccg tgtgaccagg ttgaatccgc ggtcgcgtgg aaatacggtg ttgagcgtca
    19921 ggacggcccg accgcactga tcctctcccg tcagaacctg gcgcagcagg aacgaactga
    19981 agagcaactg gcaaacatcg cgcgcggtgg ttatgtgctg aaagactgcg ccggtcagcc
    20041 ggaactgatt ttcatcgcta ccggttcaga agttgaactg gctgttgctg cctacgaaaa
    20101 actgactgcc gaaggcgtga aagcgcgcgt ggtgtccatg ccgtctaccg acgcatttga
    20161 caagcaggat gctgcttacc gtgaatccgt actgccgaaa gcggttactg cacgcgttgc
    20221 ggtagaagcg ggtattgctg actactggta caagtatgtt ggcctgaatg gcgctatcat
    20281 cggtatgacc accttcggtg agtcagctcc ggcagagttg ctgttcgaag agttcggctt
    20341 caccgtcgac aacgtcgttg cgaaagcaaa agaactgctg taattagcat ttcgggtaaa
    20401 aaggtcgctt cggcgacctt ttttattacc ttgatatgtc cgtttgcgga caagcaatag
    20461 ataaagcgtg ttgtagatca caaatattta tatgcaataa atatcaatta tgtaatatgc
    20521 atcacgatat gcgtattgac atttgttgtt atatctataa ctcaatgtta tataagaaat
    20581 taataattca ctgttttcaa aacaccggtt tccctgctca attgctttca ttaaaccgca
    20641 cgagtatttt atttttaccg ccggacggta ccctaaaaat aaaataagga taatttatgc
    20701 ggcttagtga ctattttcca gaatcatcaa tctcagttat acattcagca aaagattggc
    20761 aggaagctat cgatttctcg atggtatcat tgctggataa aaactatatc agcgagaatt
    20821 acattcaggc aattaaagat tccaccatta acaatggccc ttattatatt ctcgcaccag
    20881 gcgtggcaat gcctcatgcg cgaccggaat gtggggcgct taaaaccggg atgtctttga
    20941 cattacttga acaaggtgtt tattttccgg ggaatgacga accaattaaa ttactcatcg
    21001 gactctctgc tgccgatgcc gattcgcaca ttggcgctat tcaggcgtta agtgaattac
    21061 tgtgcgaaga agaaatactc gaacaactct taacagcatc atcagaaaaa caattagcgg
    21121 acattatcag ccgcggataa ttactctctc ctctacagga taatattatg gaaaacaagt
    21181 ctgctcgtgc aaaggtccag gcttttgggg gctttttgac tgcaatggtc atccccaata
    21241 taggtgcttt tattgcctgg ggcttcataa ctgcgttatt tattcccacc ggttggctgc
    21301 cgaatgaaca tttcgccaaa attgtcggcc cgatgattac ctatttattg cccgtgatga
    21361 ttggttctac tggtggtcat ctggtcggcg gtaaacgcgg ggcggtcatg ggcggaatag
    21421 gtactattgg tgtgatcgtt ggcgcagaga tcccgatgtt ccttggctca atgatcatgg
    21481 ggccgctcgg tgggttggtc ataaaatatg tcgataaggc actggaaaaa cgcatacctg
    21541 ccggttttga gatggttatc aataacttct cattaggtat cgcggggatg ctcctttgtc
    21601 tgctgggttt tgaagtgatc ggcccggcgg tgttaattgc caatactttc gtcaaagagt
    21661 gtatcgaggc gctggtacat gcgggttatc tgcctctgct gtcagtcatc aatgaaccgg
    21721 cgaaagtgct tttccttaat aatgcgatcg atcagggcgt ctattatccg ctgggaatgc
    21781 aacaggcttc ggttaacggt aaatccatct tctttatggt ggcctctaac ctaggtccgg
    21841 gcctggggct gctgctggcg tttaccttgt ttggtaaagg gatgagtaaa cgttctgcgc
    21901 ccggggcgat gattattcac ttcctcggtg ggatccacga actgtatttc ccatatgtgc
    21961 tgatgaagcc gctgactatt attgccatga ttgcgggcgg tatgtctggt acctggatgt
    22021 ttaacttact ggacggtggt ctggtggctg gcccaagtcc ggggtctatc tttgcttacc
    22081 tggcactgac gccgaaaggc tcgttcctgg cgacaattgc cggtgttacg gtaggtaccc
    22141 tggtgtcctt tgctattact tcgctgatac tgaagatgga aaaaacggtg gaaacggaga
    22201 gcgaagatga gtttgctcag tcagccaatg cggttaaggc gatgaaacaa gagggtgcat
    22261 tctcgttaag cagggttaag cgtatcgcct ttgtttgcga tgcggggatg ggctccagtg
    22321 cgatgggcgc gaccaccttc cgtaaacgcc tggaaaaagc ggggctggca atagaagtaa
    22381 aacattacgc gatagaaaac gtgcctgcgg atgccgatat cgtcgtcact cacgccagtc
    22441 tggaagggcg cgtgaaacgt gtgacggata aaccgctgat attgattaat aactatattg
    22501 gcgatccaaa actcgacact ttatttaatc aattaaccgc cgaacataaa cactgattac
    22561 agaggtaaaa atgaaaacca aagttgctgc tatttatggc aagcgggatg tccgtctgcg
    22621 cgaatttgaa ctgccagaaa ttaccgataa tgaactgtta gtgagtgtaa tttctgacag
    22681 cgtctgttta tcgacctgga aagcggcgtt actcggtagt gaacataaac gcgtacccga
    22741 cgatttagaa aatcatccgg tcattaccgg gcatgaatgt gccggggtta ttgtcgaagt
    22801 gggtaaaaat ctcactggca aatataaaaa aggccagcgt tttgtattgc aaccggcgat
    22861 ggggttacca agcggatatt cagcaggcta cagctacgaa tattttggcg gtaacgccac
    22921 ctatatgatt attcccgaaa tcgccattaa tttgggctgc gtattaccgt atcacggctc
    22981 ttattttgct gcggcgtcgc tggcagagcc tatgtgctgc attattggtg cttatcatgc
    23041 caattatcac accacgcaat atgtttatga gcatcgcatg ggcgtcaaac ctggcggcaa
    23101 tattgcactg ctggcgtgtg cgggtccgat gggcattggc gctatcgatt acgccattaa
    23161 cggtggcata caaccgtcac gggtggtggt ggtcgatatc gacgacaaac gcctggcgca
    23221 ggtgcagaag ctgctgccgg tggatctggc ggccagtaaa ggcattgagc tggtgtatgt
    23281 gaataccaaa gggatgagcg atccggtcca gacgctgcgg gcgctgacag gagatgtcgg
    23341 gttcgatgac atttttgttt atgcggcggt gcctgctgtc gttgagatgg ctgacgaatt
    23401 actggcggaa gatggctgtc tgaacttctt tgccgggccg acggataaaa acttcaaagt
    23461 gccgtttaat ttctacaacg tccattacaa cagcacgcac gtagtcggta catccggcgg
    23521 ttcaacggac gacatgaaag aggcgattgc gttaagcgcc acggggcagt tgcagccgtc
    23581 ctttatggtc acgcatatcg gtgggctgga tgcggtgcca gataccgtgc tcaatctgcc
    23641 ggatatccct ggcggtaaaa aactcattta taacggcgtg accatgccgc tcactgccat
    23701 tgccgatttt gccgaaaaag gcaaaaccga tccgctgttt aaagagttgg cgcggctggt
    23761 tgaggaaacg cacggcatct ggaatgaaca ggccgagaaa tatctgctgg cacaatttgg
    23821 cgttgatatc ggggaggccg cgcaatgatg tccctggcgt ggccattatt tcgcgttacg
    23881 gaacaggcag cgttggctgc ctggccgcaa accggatgtg gcgacaaaaa taaaattgat
    23941 ggcctggcgg tcaccgcgat gcgccaggca ttaaacgacg tcgcttttcg tggacgagtg
    24001 gttatcggcg aaggagaaat tgaccatgcg ccgatgctgt ggattggcga agaggtgggc
    24061 aaaggcgatg ggccagaagt cgatatcgcg gttgacccga ttgaaggcac gcggatggta
    24121 gcgatggggc agagcaatgc gctggcggtc atggctttcg cccctcgaga tagcctgtta
    24181 catgcgcccg atacgtatat gaaaaagctg gttgttaatc gactggcggc gggcgcgatt
    24241 gatctgtctt tgcctctggc ggacaacttg cgcaacgtgg cgaaagcgtt aggtaaaccg
    24301 ctggacaagc tgcgcatggt tacgctggat aaaccgcgcc tgagtgccgc gattgaagaa
    24361 gcaacacagc tgggcgtgaa ggttttcgct ctgccggatg gcgatgtcgc cgccagcgtg
    24421 ctgacctgct ggcaggataa tccttacgac gtgatgtaca ccatcggcgg tgcgccagaa
    24481 ggcgtgattt ctgcctgtgc cgttaaagca ttaggtgggg atatgcaggc cgagctgatt
    24541 gacttttgtc aggctaaagg ggattacacg gaaaatcggc agattgcaga gcaggagcgc
    24601 aagcgttgta aggcaatggg cgttgacgtt aaccgtgtgt actcgctcga tgaactggtg
    24661 agggggaacg atatcctctt tagcgctacg ggcgtgacgg gcggcgagtt ggtgaacggt
    24721 atccaacaga cggcgaatgg ggtgcggacg cagacattac tgatcggcgg cgcggaccaa
    24781 acgtgtaata taatagactc cctgcattga tggtgatcta tcggaagacc aatggcgacg
    24841 ctgacagaag atgatgtgct tgagcaactg gatgcacagg acaatttatt ttcatttatg
    24901 aaaactgcgc attctatttt gctccagggg atacgccagt ttctgccgtc gctgtttgtc
    24961 gataacgatg aagagatcgt cgaatatgca gtgaagccgt tactcgccca aagcggcccg
    25021 cttgacgata ttgatgttgc gctgcgtttg atttatgcgc tggggaaaat ggataaatgg
    25081 ctgtatgccg atatcacgca cttttcccag tactggcatt acctgaacga acaggatgaa
    25141 acgcccggat ttgccgatga catgacctgg gattttatca gcaacgtcaa tagcataacc
    25201 tgcaatgcga cgctctatga cgcgttaaaa gcgatgaagt tcgccgattt cgccgtctgg
    25261 tcagaggcgc gttttagcgg aatggtcaaa acggcgctga cgctggcagt aacgaccacc
    25321 ttaaaggaat taacgccgtg aaaattgaat taacggtgaa tgggctgaac gtgcaagcgc
    25381 agtaccatga tgaagagata gaacgcgtgc ataaaccgtt gctgcgtatg ctggcggcct
    25441 tacagacggt aagtccgcag cgacgtacgg tggtttttct ttgcgctccg ccggggacgg
    25501 gtaaatctac cttaactacc ttctgggaat atctcgcgca acaagaccct gaattacccg
    25561 ctatccagac gctcccgatg gatggttttc accattacaa tagctggctg gatgcgcatc
    25621 aattgcgccc cttcaaaggc gcaccagaga catttgacgt tgcgaaactg gcggaaaatc
    25681 tgtgccaggt agtggaaggg gattgtacgt ggccgcagta tgatcgacaa aagcatgatc
    25741 ctgttgaaga tgcgttgcac gttaccgcac cactcgtcat cgtcgaagga aactggttgt
    25801 tgctggacga cgagaagtgg tgccagcttg cgcaattttg tgatttctcg atctttatca
    25861 acgcgccagc tactgcgttg cgggaacgac tggtagggcg caagctggcg ggaggggtat
    25921 cgctggcaga cgcagaagct ttttatgacc gtaccgacgg gccgaatgtt cgccgggtgc
    25981 tggaagagag tctgcctgca aatttgacct taatgatgac cgctacgggt gaatatcgtc
    26041 tggtggatta acgttcctga ttagaccatc ttccagtcgc accacacgag agaaatgacg
    26101 ccgggtaaat gcggcgtcat gactgattgc tacgacgctc gtgcctcgct ggccgcactt
    26161 ctccagccag ctttcaaaca cgctcaacca attttcgtca aaatcccgac tcggctcgtc
    26221 cagcagcaat agtggcggag agagtgcttc caggcaggcg acggcgacca tccgtcgctg
    26281 cgcactatgc agatccaacg ggtgcgagtt ggcagtgtct gctaactgac aacattgcca
    26341 tgggtacgtt gagtgatttc atccgccggg catttttgca gtttcaaacc aaatgcgatc
    26401 tcgtcagcga cggtgctgtg aaaaagttgg ttttccgctt cctgaaacag tacgccaacc
    26461 tttgccgcgc gctggcggtt tttcaggttt ttcatcgcct gttgttgcag cataaccgta
    26521 ccggctgtgg gcctaagaag acctgccatt actcgtaaca gcgtcgattt ccctgcgcca
    26581 ttgtcaccgg tcagcgccaa ccattcacct tgtttgagtt gcagagaaat gtcgcaaaga
    26641 caatccgttg ccgcgccagg ccagcgataa gatatcttat ttaacgttaa catagtggct
    26701 ggagcgctcc gttttgcaat tgccacgctt gctggcagta gttcaggaaa ggtgtgcgat
    26761 gacgttcaaa aagaataatc agcgaaccac gttcaaacgc ccagtgttgt aagcgctgca
    26821 acagcatttc gctggcctgc ggagtcaggc ggctaaaggc ttcatcaaga attaagagct
    26881 ttggctgcat cgcgatggcg caggcgatga ccacgcgctg tgtctctcca ccggagagtg
    26941 ttgccgggtg acgatggcgc aacggctggc actcggtcag agtcagggcg gcatcaatcc
    27001 gcgccataat ctctgcttca gcaagacaca gattttctgg cccaaaggca acttcttctt
    27061 ccacgctaaa ggtacagccg gagagctgca aatagggcga ttgctggacg agctggatgg
    27121 tggcggattg ttcattaagc gggagatgcc caatgggcgt accgaggagc gtccccgtgc
    27181 ctgttatctc tcctggcaga aagtccggat accagcccgc catcagctgt gccagcgtac
    27241 ttttgccact gccgttgtca ccaaagatgg cgaccatccc cggtttgaca tagtgaaagt
    27301 cataacagaa cggaggatgc gttgagtgtg tcgggcaata acggaactgt tctaacgtaa
    27361 ccatatccac gctccgaatt ctgtcagcat cagcaagatc atggtgtaac gcgctacgcg
    27421 ttgtagggtg ctatcggctg gtgcccacaa cgttgttcgt cggttgtgga tccgaaatgc
    27481 ccgcatatcc agcgccgcac cacggatagc cagatcgttt agcgcattgt gtgttagcgg
    27541 aataattaac gcgggcatcg cacgtaaacg ctgataccag ccctcatcca gcggcacgcc
    27601 acgggcacgc tgcgcttcat gaataatcgc taattgccgt tttaactgct caacgaccaa
    27661 taacggcccg gcaaacagat aagcgacgcc tggcggcagg cgagaagcaa acagagcgcg
    27721 aataaaacat tgcacgggga cgtactgcat ccacaactgt gaggttgaaa caatcgccag
    27781 aatccgcaac cagagcgtaa tagcatgcga ccaccgttct ggcgagcgtg gcgtgccgct
    27841 tagccattct ggtagccagc caccgtgtac cagccacagc ccggctccca gcgagaacat
    27901 cagccagacg acatattttg cccgccgtcg cgtggccttt agcgcaataa ggcagaaaaa
    27961 ggtcgccgcg ctatagatcg gcaaaatggt ctgcgcgggc aggataagcg tggtgcaggc
    28021 cgccaatgcc cacagtgtta atgaggtaaa tggatgcatt tagcgcacgg cagccattgc
    28081 cggaaaatgg cgcgtggtgc gaatcggcag ctggcgcagt aaaagccaga caatcaccgc
    28141 tgtaagaatt ttatcgacca ggttcgcgcc tatcaccgta atcgccaccg actccaccag
    28201 gttttgcccc atcgagtgca tccaggcaac gaacaaatcg gctccgctac cggtgacacc
    28261 gccgaacagc gcagtacgca gcggtacagc gacgaccgtt accgcaagcg taataatcac
    28321 gccgctgaca acgacttttg gcagggtgcg aaaccaaccc gctcgcgcca gccaaccggc
    28381 gaccaggccg atgaccatcg cgacgggtgc aaatgcggcg gcgatcgggt cggtcagcaa
    28441 tccccagagt aaattggtca gtaagccggt cagcattccg atcaccggac caagcagtac
    28501 cgcgctaata agtgtgccga tggagtcgag aaatataggt aatttgacca tgctggccag
    28561 ttgcccgccg atcatattga tggcgataga tataacgatc aacaccagtg cctgactgga
    28621 aaagtggcta cgcgccataa gaaaacctct ttgaaagtag ttatttaacg ggggaaatat
    28681 gcaactcgtc gtaaccagaa agcgcgcaag gttggcggct aaacgccagt tcctcaaggc
    28741 taccgatcac cagttcaagc gtggtccgca cggtacagcc gggcatcata tgaccgccaa
    28801 gcatcgtgcc gtgtggatcg gaaacgcaga gatgaagatg ctcaccactc tgttccagcg
    28861 tgccgtttag tgagatcact tcgaatgtac cgttcaggag cgtggtgcct tcttgcccgg
    28921 catagcgcaa ggcgacgtca gtcaggctac cggtacagcc tgcaatccag gcggcatgaa
    28981 gttgatgctg ctgtgcaaaa gcgtgtagct gggaaaaaac ttcctgcccg gggaggaggc
    29041 gcagtgcgta aaagcgtgcg gaggaagaat atgggtaggg caatgtcatc attggagtac
    29101 tccgaaactc ggacgtattg cggataatat cgacgttgca tcagtcatat tctgatataa
    29161 gtcagggttt gtgacggcac aacgaaaaga ttttgctacg cttattcgtg attctgcaaa
    29221 gtggaataaa ttccacaggg gcggggctga aaacgtgacg taagtcagat aattgctttc
    29281 tttgactgga caatcattcc ttttattcca cgtttcgctt atcctagctg aagcgtttca
    29341 gtcgattaaa tgttcgacaa ttaaccaatc agtcgcagtt tgcgacaggt aaggtttccc
    29401 cggacgattt gctggattac tctgtctggc aaatttgctc agggaaaacc ttgcaggaga
    29461 tctatgaccg tacgcgtagc gataaatggc ttcggtcgca tcgggcgtaa tgtggttcgt
    29521 gctttgtatg aatccggacg ccgggcggaa attaccgtgg tggcaatcaa cgaactggcg
    29581 gatgctgcgg gcatggcgca tttgttgaaa tatgacacca gccatggccg ttttgcatgg
    29641 gaagtacgac aggaacgcga tcaacttttt gttggtgatg acgccatccg cgtattgcat
    29701 gaacgttcac tgcaatcgct cccctggcgt gaacttggcg ttgatgtagt cctcgactgc
    29761 accggcgtat atggctcccg cgagcatggc gaagcgcata ttgccgccgg ggccaaaaaa
    29821 gtgctctttt cacatcctgg cagtaacgat ctcgacgcga ccgttgttta cggcgtcaat
    29881 caggatcaac ttcgtgcgga acaccgcatc gtttctaacg cttcctgtac cacgaattgc
    29941 ataattcccg tcatcaaatt gttagatgat gcgtacggta ttgagtccgg cactgtgacc
    30001 acaattcact ccgccatgca cgatcaacag gttattgatg cataccatcc tgacctgcgt
    30061 cgcacccggg cagccagcca gtcgatcatt ccggtcgata ctaaactggc cgccggtatc
    30121 acacgatttt ttccgcaatt taacgatcgc tttgaagcga ttgcggtacg tgtgccaacc
    30181 ataaatgtga cggcaatcga tttaagcgtg acggtgaaga aacctgtaaa agccaatgaa
    30241 gtcaacctgt tgctgcaaaa agcagcacaa ggtgcatttc atggtatagt tgactatacg
    30301 gaattgccgt tggtctctgt agattttaac cacgatccgc acagtgccat tgtcgatggc
    30361 acccaaaccc gggtcagtgg cgcacacctg atcaaaacgt tggtctggtg cgataacgaa
    30421 tggggctttg ctaaccgaat gctcgacacg acgttagcta tggctactgt tgctttcagg
    30481 taagacgcaa gcagcgtctg caaaactttt agaatcaacg agaggattca ccatgtctgt
    30541 aattaagatg accgatctgg atcttgctgg gaaacgtgta tttatccgtg cggatctgaa
    30601 cgtaccagta aaagacggga aagtaaccag cgacgcgcgt atccgtgctt ctctgccgac
    30661 catcgaactg gccctgaaac aaggcgcaaa agtgatggta acttcccacc tgggtcgtcc
    30721 taccgaaggc gagtacaacg aagaattctc tctgctgccg gttgttaact acctgaaaga
    30781 caaactgtct aacccggttc gtctggttaa agattacctc gacggcgttg acgttgctga
    30841 aggtgaactg gttgttctgg aaaacgttcg cttcaacaaa ggcgagaaga aagacgacga
    30901 aaccctgtcc aaaaaatacg ctgcactgtg tgacgtgttc gtaatggacg cattcggtac
    30961 tgctcaccgc gcgcaggctt ctactcacgg tatcggtaaa ttcgctgacg ttgcgtgcgc
    31021 aggcccgctg ctggcagctg aactggacgc gctgggtaaa gcactgaaag aacctgctcg
    31081 cccgatggtg gctatcgttg gtggttctaa agtatctacc aaactgaccg ttctggactc
    31141 cctgtctaaa atcgctgacc agctgattgt tggtggtggt atcgctaaca cctttatcgc
    31201 ggcacaaggc cacgatgtgg gtaaatccct gtacgaagct gacctggttg acgaagctaa
    31261 acgtctgctg accacctgca acatcccggt tccgtctgat gttcgcgtag caaccgagtt
    31321 ctctgaaact gcaccggcta ccctgaaatc tgttaacgat gtgaaagctg acgagcagat
    31381 cctggatatc ggtgatgctt ccgctcagga actggctgaa atcctgaaga atgcgaaaac
    31441 cattctgtgg aacggtccgg ttggcgtgtt cgaattcccg aacttccgca aaggtactga
    31501 aatcgtggct aacgctatcg cagacagcga agcgttctcc atcgctggcg gcggcgacac
    31561 tctggcagca atcgacctgt tcggcattgc tgacaaaatc tcctacatct ccactggcgg
    31621 cggcgcattc ctcgaattcg tggaaggtaa agtactgcct gcagtagcga tgctcgaaga
    31681 gcgcgctaag aagtaaaaaa tcacagggca gggaaacctg cccttgtttc agcgcgcttt
    31741 tagagcacgc attttttcat actctaaata attcgagttg caggaaggcg acaagagagt
    31801 gaatccccag gagcttacat aagtaagtga ctggggtgag cgaacgcaga cgcagcacat
    31861 gcaacttgaa gtatgatgag tataaggccc gacgatacag gacaagagac atgtctaaga
    31921 tttttgattt cgtaaaacct ggcgtaatca ctggtgatga cgtacagaaa gttttccagg
    31981 tagcaaaaga aaacaacttc gcactgccag cagtaaactg cgtcggtact gactccatca
    32041 acgccgtact ggaaaccgct gctaaagtta aagcgccggt tatcgttcag ttctccaacg
    32101 gtggtgcttc ctttatcgct ggtaaaggcg tgaaatctga cgttccgcag ggtgctgcta
    32161 tcctgggcgc gatctctggt gcgcatcacg ttcaccagat ggctgaacat tatggtgttc
    32221 cggttatcct gcacactgac cactgcgcga agaaactgct gccgtggatc gacggtctgt
    32281 tggacgcggg tgaaaaacac ttcgcagcta ccggtaagcc gctgttctct tctcacatga
    32341 tcgacctgtc tgaagaatct ctgcaagaga acatcgaaat ctgctctaaa tacctggagc
    32401 gtatgtccaa aatcggcatg actctggaaa tcgaactggg ttgcaccggt ggtgaagaag
    32461 acggcgtgga caacagccac atggacgctt ctgcactgta cacccagccg gaagacgttg
    32521 attacgcata caccgaactg agcaaaatca gcccgcgttt caccatcgct gcgtccttcg
    32581 gtaacgtaca cggtgtttac aagccgggta acgtggttct aactccgacc atcctgcgtg
    32641 attctcagga atatgtttct aagaaacaca acctgccgca caacagcctg aacttcgtat
    32701 tccacggtgg ttccggttct actgctcagg aaatcaaaga ctccgtaagc tacggcgtag
    32761 tgaaaatgaa catcgatacc gatacccaat gggcaacctg ggaaggcgtt ctgaactact
    32821 acaaagcgaa cgaagcttat ctgcagggtc agctgggtaa cccgaaaggc gaagatcagc
    32881 cgaacaagaa atactacgat ccgcgcgtat ggctgcgtgc cggtcagact tcgatgatcg
    32941 ctcgtctgga aaaagcattc caggaactga acgcgatcga cgttctgtaa gatattcctt
    33001 tctgcttatc tcaaggcccg ctctgcgggt ctttttttcg ccaaaagcaa aattgcctga
    33061 tgcgctacgc ttatcaggcc tacacgtatc ctgcaatata ttgaatttgc aggtttttgt
    33121 aggccggata aggcgttcac gccgcatccg gcatgacaaa gcgcacattg atatctgtct
    33181 gagccccgct ctgcggggat tttttcgcca aaagcaaggt gattcagatg agaaatctgt
    33241 gatctatttg gcaaaattat gctttattgt ttacccttgt cagactgccc gtcataaggc
    33301 ggcggagtgt atttctccat tttgagtcag ttgaaaagga atattgaatg gaagatttga
    33361 atgttgtcga tagcataaac ggcgcgggaa gctggctggt agctaaccag gcgctgctgc
    33421 taagttatgc agtaaacatc gtggcggcac tcgcgatcat catcgttggt ttgattatcg
    33481 cgcggatgat ttccaacgcg gtgaatcgcc tgatgatctc ccgtaaaatc gatgccactg
    33541 ttgctgattt tctttctgca ttagtccgtt acggtattat cgcctttacg ctaatcgctg
    33601 ccctgggacg cgtgggtgta caaacagcgt cagtcattgc tgtactcggt gccgcaggct
    33661 tagctgttgg tctggctttg caggggtcac tttctaacct ggccgctggc gtgttacttg
    33721 tcatgttccg cccgttccgt gccggagaat atgttgacct cggcggcgta gccggtactg
    33781 tgctgagtgt gcagattttc tccaccacca tgcgtactgc agacggtaaa attatcgtta
    33841 ttccgaacgg taaaattatt gccggaaata ttattaactt ctcccgcgag ccagttcgcc
    33901 gtaacgaatt tattattggc gtggcgtatg attccgatat cgatcaggtt aagcagatcc
    33961 tgaccaatat tatccagtct gaagatcgca ttttgaaaga tcgcgaaatg actgtgcgcc
    34021 tgaacgaact tggtgcatcg tcgattaatt tcgtggtccg cgtctggagc aacagcggcg
    34081 atctgcaaaa cgtgtactgg gatgtgctgg agcgtattaa acgtgaattt gatgccgccg
    34141 gtatcagctt cccgtacccg caaatggatg tgaactttaa gcgggtgaaa gaagacaaag
    34201 ctgcgtaatc aacgctgagg ccagataata ctctctggcc tctctcttat tagttattct
    34261 gattgccaat taatattatc aatttccgct aataacaatc ccgcgatata gtctctgcat
    34321 cagatactta attcggaata tccaacgtgt tttcttatta ctttcaaggt cttgctcttg
    34381 gggcagctat gattctgccg ctcggcccac aaaatgcttt tgtgatgaat cagggcattc
    34441 gtcgtcagta ccacattatg attgccttac tttgcgctat cagcgatttg gtcctgattt
    34501 gcgcggggat ttttggtggt agcgcgttat tgatgcagtc gccgtggttg ctggcgctgg
    34561 tcacctgggg cggcgtagtc ttcttgctct ggtatggttt tggcgctttt aaaacagcga
    34621 tgagtagtaa tattgagtta gctagtgccg aagtcctgaa gcaaggcaga tggaaaatta
    34681 tcgccaccat gttggcagtg acctggctga atccgcatgt ttacctggat acttttgttg
    34741 tactgggcag ccttggcggg cagcttgacg tcgaaccaaa acgttggttt gcgctcggga
    34801 caattagcgc ctctttcctg tggttctttg gtctggctat tctcgcagcc tggctggcac
    34861 cgcgcctgcg cacggcaaaa tcacagcgca ttatcaatct ggtagtggga tgtgtcatgt
    34921 ggtttatagc cttgcagctg gcgagagacg gtattgctca tgcacaagcc ttgttcagtt
    34981 aggcgctatc tgatggaaaa ataaaacaga ggcgctaagc ttgcctccag aggtcctgag
    35041 ttttgggcaa gtcaattaac acggagagac taacgtgaag ttcaaagtta tcgccctggc
    35101 ggcattaatg ggtattagcg ggatggcagc gcaggctaac gaattgccgg atggaccgca
    35161 tattgtcacc tccggtacgg caagtgtgga tgcggtgtca gacattgcca ctcttgcgat
    35221 tgaagttaac gtggccgcga aggatgccgc tacagccaag aaacaggcag atgagcgcgt
    35281 cgcacaatac atttccttcc ttgaactcaa tcagatcgcg aaaaaagata tcagctcagc
    35341 gaatttacgc acccagccag attatgatta tcaggatggt aaaagtatcc tgaaaggcta
    35401 ccgcgctgtg agaacggtgg aagtcacgct ccgtcagtta gacaaactga attccttgct
    35461 ggatggcgcg ctgaaggcgg gtcttaacga aattcgttct gtgtcgctgg gcgtggcgca
    35521 gcctgatgcc tataaagaca aagcgcgtaa ggcagcgatt gataacgcga ttcatcaggc
    35581 gcaggaactg gcgaacggct ttcatcgtaa actggggccg gtatatagcg tgcgctacca
    35641 tgtttccaac tatcagccca gcccaatggt gcggatgatg aaagccgatg ccgcgccggt
    35701 gtctgcccag gaaacttacg agcaggccgc tattcagttt gatgatcagg tcgatgtggt
    35761 cttccagtta gaacctgtgg atcaacaacc cgctaaaaca cctgcagcac aataatactt
    35821 gtagcccgac gtattcgtcg ggctaatgcc tttacccgat attgcctcct ttttctgtaa
    35881 ttttctgtaa aaccatgacg taatcaccta tctttgctcg cggtttcatc aaggataggc
    35941 tgactgaaac ggttaagcgg acaaggatgc ggggaggggg gatggacatt tttatttcga
    36001 aaaagatgcg caatttcatt ttattagcgc aaaccaataa tattgcccgg gcggcagaaa
    36061 aaatccatat gtcggcttcg ccatttggta aaagcattgc cgcgctggaa gaacaaattg
    36121 gctatacgct atttacccgc aaagataata atatcagcct caataaagcc ggtcaggaac
    36181 tgtatcaaaa actgttcccg gtttatcaac gactttctgc gattgataat gaaatccata
    36241 actcagggcg tcgttcacga gaaattgtga ttggcataga taatacttat ccgacaatta
    36301 ttttcgatca gttgattagt ctcggagata agtatgaagg cgtgaccgcc cagccagttg
    36361 agttcagcga aaacggcgtt atcgataatc tttttgaccg tcagttagat tttattatct
    36421 ccccacagca tgtatctgca cgagttcagg aactggaaaa cctgactatc agtgaattac
    36481 caccgctgcg attgggcttt ctcgtttctc gccgttatga agagcgtcaa gagcaagagc
    36541 tgcttcagga gctgccctgg ctacaaatgc gctttcaaaa ccgggctaac ttcgaagcaa
    36601 tgattgatgc gaatatgcga ccttgtggga tcaatcccac cattatttat cgtccctaca
    36661 gttttatggc caaaatcagc gccgtggagc gcggacattt tctgacggtg atcccgcatt
    36721 ttgcctggcg actggtgaat ccggcaacgc tgaaatattt tgatgcgccg cacaggccga
    36781 tgtatatgca ggaatatctt tattcaatca gaaatcatcg ttataccgcc acgatgcttc
    36841 agcatattgc tgaagatcgt gacgggacaa accattaacc cagcatcgag ccggttgcaa
    36901 ttaaattacg gtgtaagtcg aagacgtggc taagatcgtg gtgaatatgt ccgccgggcg
    36961 cattttccag atagcgatgc agataatccc gatacatagg atgtgcacaa ttatcaatga
    37021 tagtgcgggc gcgttgaagc ggggaaagac cgcgcagatc ggcgatccct tgttcggtga
    37081 tgatcacttt gacgctgtgt tcgctgtgat caacatggct gcacattggc acgacggttg
    37141 agatcttgcc ttctttagca atcgacggag ccataaagat cgacagatac gcgttgcgtt
    37201 caaaatcacc gctgccgccg atgccgttca tcagatcgac cccggctacg tgcgttgagt
    37261 tggcatgccc gtaaatatca aactccaggc cgacgttcag agcgatgacg cccagacgac
    37321 ggatgatttc cgggttattg gaaatctcct gcggacgcaa cacaatgcgg ctggcaaagt
    37381 aatccatatt gtcgtaaatc ttgcgcaggg aatcggccga gattgtcagg ctggaggcgc
    37441 tggccccgct gattttgccg gtttccagta aatgcaccac cgattcctgt agcacttccg
    37501 aatacatcat aaacggagga atttccgggt tttcccccag acgcgccatt accgcattat
    37561 tgatattgcc cacgccactt tgcagcggca gaaattccgg cggaatacgc ccatgcgcca
    37621 tttcctgcaa taagaacgtg accacgttat cggcaatctg ctggcacatg ggattttgct
    37681 tatccagcat attaccggcg tcgggcaagt tggtttccac gacggcgaca atctttttcg
    37741 gatcgatttg cacatagcgg gtaccgacgc gatccattgc atggaagatc gacacgctat
    37801 tgcgccgcgg tggcgcgcca ggaatcacaa tatccgccag ttctgcaacg cgcggatcgt
    37861 gatagtgatt aagttcaatg atcactttct tcgcccgcag cagccatgtc ggcgcattac
    37921 cgatcccgct ggttaaccag actcgaccat ccggtgccag tgccgatgct tcgatgacgg
    37981 caacatcaat gtcgccgaag aaaccgtaat tgaccatttg cgccacttcg ctcaaatgca
    38041 ggtcaacgaa actcaccgcg ccctgattga tctttttacg taaaccggac gatgtttgat
    38101 atggcgcacg ccaggaaaca gcatcggcgt cagaaagtac atcgtcagcg gcggcgctga
    38161 ttgacgcccc cgtcagcagg cgaatttgat acggcttttt ggcctcatgc tgttcgttag
    38221 ctctgcgggc aatcgcggtc ggtaaggctt tcggcgaacc cgctggggta aagccgctaa
    38281 atgccaccat gtcgttatgc tggataattt ctgccgcttc atcggcggtc atccttgtcc
    38341 actgagtttc catttttact ccttcgttcg cagggattaa tgaccaacga aattaggttt
    38401 acgtttttcg aggaaagcgt tcatcccttc ctggtaatct tcgctgtcat aaaccgcgcg
    38461 gcgcatcccc tgaatacgtt caaattcatc ggagttcatg gtgtgcgctt cgcccagtac
    38521 acgcagctct tctttgataa cagcaatggc taacggcgct ttctcagaga tgtggtgcgc
    38581 catttgtaag gtgaaatctt ccagttcttc cacttccaca acatggttga ggatgccgac
    38641 agccaacgcg cgctgggcgg tgattggcga agcggtaaaa atcagttctt tgacaatgtg
    38701 gaagcccgcg tcgcgggtca ggttgtgaat gccgaccagg ttatacggga cgccgaggtt
    38761 tacaggcgtc attgagaagg ttgaggtact ggcggcgata atcagatcgg aactcatgat
    38821 catttcaaat gcgccacccc aaacactacc ttccaccatc gaaatgatcg gtttcgggaa
    38881 tttttggatc atgcgggtga tttgacgcaa tggatcatca taggagagcg gatcgcgacc
    38941 gccagacggc agttcgtgaa tagcgtggcc tgcggagaag actttggatc cactcggcgc
    39001 gcgcaaaatg atacagcgaa tttccggccg gttgagatcg cttaatgcct gcataagatc
    39061 atcaataaag actttactta aggcattaag tttgcggcca tagttaaact caatgaccgc
    39121 cactttgttg atagtgacaa cgttaacata ctgataagac ataaaaattc ctttaatcaa
    39181 aatattgcgt ctggataaat tcactgagct gccgcaggcc ggtgcgcggt gagagcgtat
    39241 tgtttttgac cgctaaaagc gtctggcggt aatagcgatc gaaatcttca ttcgcgaaca
    39301 ggtgattcag tacttcttct tcggtctgct tacgcagcca ttccaccgat tgttgttgcc
    39361 gcacttgttg taaacgacca ctggcagtta gcgcggtttt gaagtcgatg atggcgtgcc
    39421 agatctcatc gattccacgt ttttccagtg cgctacaagt cagaacccgt ggctgccatt
    39481 cgtcgtattt acgtcgcaga atatgcaggg cactctcgta catatgccgg gcaatggcga
    39541 cattggtatg gttatcgcca tcgtctttgt tgataacgat cagatcagcc acttccatca
    39601 gccctttttt aatgccctgc agatcatcgc cgccaccggc aatttgcaac gagataaaac
    39661 agtccaccat gcgggcgact tctgtttccg actgcccgac gccaaccgtt tcgacaatca
    39721 ctacgtcata acccgctgct tcgcataaca gcattaattc ccgcgctcgc tgactggcac
    39781 cgcccagatg accggaggat ggtaccgggc gaataaacgc cgcttcggca cgcgccaggt
    39841 cattcatgcg ggttttatcc ccgagaatgc taccgccagt gaccgggctg ctgggatcga
    39901 ccgcaataac cgcgaccttt aatccctctc gaatcaacaa catgccaaag gcctcaagaa
    39961 aggtactttt ccccgcgccg ggggtgccgg taacgcccag tcgcagggtg ttaccgcagt
    40021 acggcataat ggcatcaagc agctgcgtac ttagtgcctg atgacgcggg tgacggcttt
    40081 ccaccagcgt catggcctgg gcgagtgtgg cacgctcacc ctgacgtaag cggcgaatac
    40141 tttctgccag cgtggcttca ttaatcatga tgctggctta tcagattcag tacgtcgcgc
    40201 acactgtcga gcataggtgt acctggacca taaatcgccg ccacgccgcg ctcttgcagg
    40261 aaggcgtaat cctgcggcgg aatgacgcca cccgcgacca cgcagatatc ttcgcgtccc
    40321 cattttttca gcgcttcgac cagttccggg atcagcgttt tatgaccggc agccagtgag
    40381 gatgcgccca ctacgtgaac gtcgttttct acggccaggc gggcgatctc ttcaggtgta
    40441 gagaacatcg ggcttaaatc tacgtcgaaa ccgagatcgg aataggcgct ggcgatcact
    40501 ttcgcgccgc gatcgtgtcc atcctggccc atcttagcga tcagaatgcg cgggcgacga
    40561 ccattgtcgg caaggaactg ctccgtttgc gcaacaatgg catcgaactc ggaggccgat
    40621 ttctcagact gatgatagct ttgcgcaatc acgccggtaa cacactggct tggcaccaga
    40681 taacggtcga aagcgacttc cagcgcatcg gaaatttcac ccagggtggc gcgaacgcga
    40741 gcggcattaa cagcggcagc cagcaggttt tcgttatgct gtgcggcgtg agtcagggcg
    40801 ttcaacgcgg cggttacggc ggcatcatca cgggtggcgc gaatgcgttc cagcgaagca
    40861 atttgctcgt tacgcaccat cacgttgtcg atctcaagta catcggtttc gtcttcgtga
    40921 tccagtttgt acttgttgac accaacgatg acacgcttgc cctggtcgat cagcgactgt
    40981 tcgcgcgctg aggcctcttc gatcattcgt tttggcagac ctgcttcgat cgctttcgcc
    41041 atgccaccgg cttcgtcgat ctgttggata atagctctgg cttgtttgac gatttgatcg
    41101 gtcagcgact caatgtaata ggatccggcc agtggatcga cgatgcggca gagttctgat
    41161 tcttcctgga tgatgatctg ggtgttgcgg gcaatgcgtg ctgagaaatc ggtaggcaaa
    41221 ccaagcgctt cgtcaaaggc gttggtatgc agtgactgag taccgcccag cgtcgcagcc
    41281 agcgcttcaa tggtggtgcg gataacgttg ttatacggat cctgttcagt caggctccag
    41341 cctgaggtct ggcagtgggt acgcagcgcc agtgatttcg ggtcctgtgc gccaaatcca
    41401 ctgaccgctt cgctccataa ataacgtgcc gcacgcaaca tggcgacgtt cataaacaga
    41461 tccatgccga tgccgaagaa gaacgacagg cgaggagcga agtcatcaat tttcagtccg
    41521 gcagagattg ctgctttgat gtactcaatc ccatcagcga gcgtaaatgc tacctgctgc
    41581 acgcagttgg cacccgcttc acccatgtgg taaccgctga tactgatggt attaaatcgc
    41641 ggcatgttgc cggaacacca ggcgatgatg tcggcgataa tgcgcattga cggttttggc
    41701 gggtaaatat aggtgttgcg gcagaggtac tctttgagga tatcgttctg aatggtgccg
    41761 gtcagtttat caggtgtaac accttgctct tccgcggcga tgatataaaa cgccagtact
    41821 ggtagcactg cgccattcat ggtcatcgaa accgacattt tatccagcgg gatctggtcg
    41881 aacaggactt tcatatcttc cacggtgtcg atagcgacgc ccgctttgcc gacgtcaccc
    41941 gccacgcgtg ggttatcgga gtcgtagcca cggtgggtgg caaggtcaaa cgcaacggaa
    42001 agaccttttt gcccggcggc caggttacgg cgataaaaag cgttggactc ttttgctgtt
    42061 gaaaaaccag catactgacg gatggtccac ggttgggcgg tatacatagt ggcacgcggg
    42121 ccacgaacgt agggcggcaa accaggaagg gtacctgtca cctccagatt atcgagatcg
    42181 gcttcggtat acagcggctt gatggcgatc ccttccgcgg tttgctgaac cagcgagtcg
    42241 acagttttct cccgacggct caattccttg ttggcaagct gttgccactc ctgctcgtta
    42301 gacattattt gctccgtaaa acgcaatcaa aatgagggta tttgcgctta cggtgagctt
    42361 tttacgtggg tttgtcgcca gcaaaaaaaa tgcattcaga cgtcgttttt tgccgcctga
    42421 gatttgtcta ttttgtgatg tgattcaagg aatacaaaaa gaagtgagga taacgcctga
    42481 tatgtgctat caggcattat ttgatggatt aatcctgacg aaggactttg tgaccataat
    42541 cgagcaacgc atcagtgact ttacgcatca tgcggctttc aggagcaaag cggtgccagt
    42601 agagcatccg gcgttgaaat agccctggcg ttaagtcaat cagttcaccg ctggctagtt
    42661 ctttctcgat ttgcaggtgc gggatcatac agcaggtggt gccctggcga gcaagttgca
    42721 cgaacgcttc tgaagaatta acgatatggc agggcacgct gcctggaggc agatcgaagt
    42781 tttgctgcaa aaaggcctgg tgcatatcgt caagatggtc aaaggcgacc acaggtgctt
    42841 tcaataatgc cgaacgcgtt acgccgttag ggaaatattt ttcggcaaag ggttttgagc
    42901 tgacgaacag atagtcgagc gcaccaagtt tatcgacaag acaactcggc agcgcctgat
    42961 gttgaatact caccgcgccg accacttcgc cgcggcgcag acgttcctga gtgcgggttt
    43021 catcttctac ctgcaagttg aggcggatag gcgaatcagc caacacagga gccagtgcag
    43081 gaagcaacca cgtcgccaga ctgtcggcgt tgaccgccag tgaaagcagc agcggagtcg
    43141 aaccggtttg ttcatcgccc agccactctt cttccagcaa ctccacctgg cgcagcagtg
    43201 ccagcagttt ttgcccttgt tccgtcgggc gcggcggtac ggtacgcacc aacagcggct
    43261 gcccgaacat attttccagt tgcttaatgc gctgtgagac ggctgattgt gtaatgcaca
    43321 gcttttgtgc cgcgcgctca aatcctcgtt cacgtatcac cgcatccagt gcctgtaatg
    43381 ttctgtagtc cgggcgtttc attgctgttg tctgctcctg gtaaatgctt tttcctgcac
    43441 tatgacacaa ttttgtgtca ggttgcatat accgaagccg tcggcgggta acatttgtat
    43501 tcgtggttaa acgcgcagct taaaagatca gggatttgcg ctaatgcatt tcgctcagcg
    43561 agttcgcgcc ctggttgtgt tgaatggcgt ggcgttattg cctctgttcg caccgtggag
    43621 cggcataccc agaccgttgt atgctttatt tgcggggcga aaggggatgc ccgccattgc
    43681 gcgatatttt atggatgagt taaccacgcg gcttgccaac ggggtctgaa tcgctttttt
    43741 tgtatataat gcgtgtgaaa tttcatacca caggcgaaac gatcatgacg caggatgaat
    43801 tgaaaaaagc agtaggatgg gcggcacttc agtatgttca gcccggcacc attgttggtg
    43861 taggtacagg ttccaccgcc gcacacttta ttgacgcgct cggtacaatg aaaggccaga
    43921 ttgaaggggc cgtttccagt tcagatgctt ccactgaaaa actgaaaagc ctcggcattc
    43981 acgtttttga tctcaacgaa gtcgacagcc ttggcatcta cgttgatggc gcagatgaaa
    44041 tcaacggcca catgcaaatg atcaaaggcg gcggcgcggc gctgacccgt gaaaaaatca
    44101 ttgcttcggt tgcagaaaaa tttatctgta ttgcagacgc ttccaagcag gttgatattc
    44161 tgggtaaatt cccgctgcca gtagaagtta tcccgatggc acgtagtgca gtggcgcgcc
    44221 agctggtgaa actgggcggt cgtccggaat accgtcaggg cgtggtgacc gataatggca
    44281 acgtgatcct cgacgtccac ggcatggaaa tccttgaccc gatagcgatg gaaaacgcca
    44341 taaatgcgat tcctggtgtg gtgactgttg gcttgtttgc taaccgtggc gcggacgttg
    44401 cgctgattgg cacacctgac ggtgtcaaaa ccattgtgaa atgatctgac gggggaacct
    44461 cccccgttaa aaaaattctc ttcattaaat ttggtgacat gtgtcacgtt tttacctggc
    44521 tattgtcgat tgctctaaat aaatcttcta aaccagcata ttcatccaag aattaccttt
    44581 gcgtgatatt tcctcaacat cgcgacgcaa acgttcatat tgccgcaata ttattttttg
    44641 atatgttgaa aggcggacgc aaatccgcac acaacatttc aaaagacagg attgggtaaa
    44701 tggcaaaggt atcgctggag aaagacaaga ttaagtttct gctggtagaa ggcgtgcacc
    44761 aaaaggcgct ggaaagcctt cgtgcagctg gttacaccaa catcgaattt cacaaaggcg
    44821 cgctggatga tgaacaatta aaagaatcca tccgcgatgc ccacttcatc ggcctgcgat
    44881 cccgtaccca tctgactgaa gacgtgatca acgccgcaga aaaactggtc gctattggct
    44941 gtttctgtat cggaacaaac caggttgatc tggatgcggc ggcaaagcgc gggatcccgg
    45001 tatttaacgc accgttctca aatacgcgct ctgttgcgga gctggtgatt ggcgaactgc
    45061 tgctgctatt gcgcggcgtg ccggaagcca atgctaaagc gcaccgtggc gtgtggaaca
    45121 aactggcggc gggttctttt gaagcgcgcg gcaaaaagct gggtatcatc ggctacggtc
    45181 atattggtac gcaattgggc attctggctg aatcgctggg aatgtatgtt tacttttatg
    45241 atattgaaaa taaactgccg ctgggcaacg ccactcaggt acagcatctt tctgacctgc
    45301 tgaatatgag cgatgtggtg agtctgcatg taccagagaa tccgtccacc aaaaatatga
    45361 tgggcgcgaa agaaatttca ctaatgaagc ccggctcgct gctgattaat gcttcgcgcg
    45421 gtactgtggt ggatattccg gcgctgtgtg atgcgctggc gagcaaacat ctggcggggg
    45481 cggcaatcga cgtattcccg acggaacctg cgaccaatag cgatccattt acctctccgc
    45541 tgtgtgaatt cgacaacgtc cttctgacgc cacacattgg cggttcgact caggaagcgc
    45601 aggagaatat cggcctggaa gttgcgggta aattgatcaa gtattctgac aatggctcaa
    45661 cgctctctgc ggtgaacttc ccggaagtct cgctgccact gcatggtggt cgtcgtctga
    45721 tgcacatcca cgaaaaccgt ccgggcgtgc taactgcgct gaacaaaatc ttcgccgagc
    45781 agggcgtcaa catcgccgcg caatatttac aaacttccgc ccagatgggt tatgtagtta
    45841 ttgatattga agctgacgaa gacgttgccg aaaaagcgct gcaggcaatg aaagctattc
    45901 cgggtaccat tcgcgcccgt ctgctgtact aattcccctt ctctgaaaat taacgggcag
    45961 gtcactgact tgcccgtttt tttatccctt ctccacaccg ttgccgcgtt gcgcattcta
    46021 ctttgcgagt ctgctccaga aaataaatca aagtcagatc aatgcgttgt gtttatttaa
    46081 tggggattta cgatggcggg acagcatggg gctgtaacgg taaagccctc accgaagcga
    46141 gggcttaaag gagaagggtt atgatgcgac ttgtcatcat actgattgta ctgttactga
    46201 taagtttcgg agcttattaa cagtcagtct caggggagga gtcatcctcc cttaccctta
    46261 ctcactaaat taggtcaaag aatcaacgat gtcaatcagg gcgatgcggg gtgcatcgcc
    46321 ctgaaccgaa ctttttgata gccacttcat cgcccacgtt tactgccgta atagatcctt
    46381 tttcccaaaa attatctctg ttttgcatgg ggtttttcga gcacgctcga agaaaagatt
    46441 taaaggtaga tcaatgcgtt gtggttattt aaagagagct tacgatagct gggcagcatg
    46501 gggctgtaac ggtaaagccc tcaccgaagc gagggcttga aggagaaggg ttatgatgcg
    46561 acttgccatc atactgattg tactgttact cataagtttc ccagcttatt aacagtcagt
    46621 cacaggggag gagcaatcct cccttaccct tactcactaa attaggttaa agaatcaacg
    46681 atgtcaatca gggcgatgca ggtgtatcgc ccttaccact cccagacttt cgacggtgta
    46741 accaccgcag gaagagggat atcccactct tcaacgggga gtttttccac caactgacaa
    46801 tcatgcgcat aacccaccgg ttgcgtttta tagtgctgcc agttttgtaa ggtccgatca
    46861 taaaaaccac cgcccattcc caggcgctga ccgtactcat caaaggcgac cagcggtgtg
    46921 atcagcacgt ctaatcggga aaggggtagc acgtcacgca catccagttt tggctcatgg
    46981 atcttcaacc tgttcatcac cagttcgctt tgcggatggt aattcaggaa cagcaaatta
    47041 ccggcactaa agggatgcaa aactggaaga tatacgcgct taccggcgcg ccagagttgt
    47101 tctattagcg gctgggtgtc gagttcacca tcaaaagaga ggaatacagc gaccgtatgc
    47161 gccatcacca ccgggggata agtcatcatc cgggtagcgg cttgttgacc catttcctgc
    47221 tgttgttccg gcgttaacgc acgacgacgt tgccgaatca ttttgcggat ttcttgtcgg
    47281 gataatgtca gtgggagttc tggtagttgc gtcatggctg gtgccagata agaagggaat
    47341 ctccgagatg ccgccgcagg ctgtaaccct tgaacccttg gttcaaggtg aatgtgtcgt
    47401 cgcagtttta aggcttctcg gacggaccga gcatgctcac caaccgcgga gcgccacatt
    47461 cttgtggtat gaaatatcgg ctcaggggac tggcccgctt gcgaacatct cagagaaatt
    47521 ttgtcttcac ggttactcta ccacagtaaa ccgaaaagtg tcattcaaag ttttggttag
    47581 ttttttcggt gatgcgacct tgttcaagta acgcttgttc tatggtctgc tgcagcatcc
    47641 gaatacgctg ttccatactt gccgcgtagt cacgagtctt cgctttttct tgcgctaact
    47701 catagctgat attcaatgcg gcaatgaaga ccaactgttc agtatttgtg actctagtgc
    47761 gttctttcag atcttgcaac cgttggttca gatcgtccgc tgcctgattc aacgcatccc
    47821 tttggtcagg cgggcagttc acacgcagtg aacggccaaa aatttggata tcgacgggtt
    47881 gtgcagacat gccaccttcc tgctgattga ctgcgctgcc ttcgtcttta gacccatggt
    47941 ctgcgaaggg gcgacactat agctaccctg atgagaagag acaagccctt ttctggtcca
    48001 ccagggccca aagtggtagc atatcatgaa tattcctccc tttgacgacg aatgcttatg
    48061 tctatacaga acgaaatgcc tggttacaac gaaatgaacc agtatctgaa ccaacaaggg
    48121 acgggtctga cccctgctga gatgcatggt ttaatcagtg ggatgatatg tggcggtaac
    48181 gatgacagct catggctacc gctacttcac gacctgacga acgaaggcat ggctttcggt
    48241 catgagctgg cacaggcact gcgtaaaatg cactctgcca ccagcgatgc cctgcaggat
    48301 gacggcttcc tttttcagct ttatctgcct gatggcgatg atgtcagcgt tttcgatcgg
    48361 gctgatgcat tggcaggttg ggtcaatcac ttcctgcttg gtcttggcgt tacgcaaccg
    48421 aagctggata aagtgaccgg cgaaaccggt gaagctatcg acgatctgcg taacattgcg
    48481 caactgggtt acgacgaaga cgaagatcag gaagagcttg aaatgtcgct tgaagagatc
    48541 atcgaatacg ttcgtgttgc cgcgctgtta tgccacgaca cctttactca tccgcaaccg
    48601 accgcgccag aagtacaaaa accgactcta cactaaaaac aaaaaacgta aggagagtgt
    48661 tatgagtgag atatcccggc aagagtttca gcgtcgccgt caggccctgg tggagcaaat
    48721 gcaacccggc agcgccgcgc tgatttttgc tgcaccagaa gtaacacgta gcgccgacag
    48781 cgaatacccc tatcgtcaga acagtgactt ctggtacttc accggcttta acgaaccgga
    48841 agcggtgctg gtgctgatta aaagcgatga cactcataac cacagcgttc tgtttaaccg
    48901 cgttcgcgac ctgacggcgg agatctggtt tggccgtcgc ttaggccagg atgccgcgcc
    48961 agagaaactg ggcgttgacc gcgcactggc attcagcgaa atcaatcagc aactttatca
    49021 actacttaac ggcctggatg tggtttacca tgcccagggc gaatatacct atgctgatga
    49081 aatcgtgaac agtgcgctgg aaaaactgcg taaaggttcg cggcaaaatc tcaccgcacc
    49141 ggcaacgatg atcgactggc gtcctgttgt tcatgaaatg cgcctgttca aatcgccaga
    49201 agagattgcc gtactccgcc gcgcgggaga aatcaccgcc atggcacata cacgggcgat
    49261 ggaaaaatgc cgtccgggaa tgttcgagta ccatctggaa ggcgaaattc accacgaatt
    49321 taaccgccac ggtgcgcgct atccgtccta taacaccatt gtcggcagcg gtgaaaacgg
    49381 ctgcattctg cactacaccg aaaacgagag tgaactgcgc gacggtgacc tggtgttgat
    49441 tgacgcgggt tgtgaataca aaggttacgc gggcgatatt acccgcacct tcccggtcaa
    49501 cggcaaattc acccaggccc agcgtgaaat ctacgacatt gtgctggagt ctctcgaaac
    49561 cagcctgcgc ctgtatcgtc cgggaacttc cattctggaa gtcactggtg aagtggtgcg
    49621 catcatggtt agcggcctgg taaaactcgg catcctgaaa ggtgatgttg atgaactgat
    49681 cgctcagaac gcccatcgtc ctttctttat gcatggcctt agccactggt taggactgga
    49741 tgtccatgac gttggcgttt atggtcagga tcgctcgcgc attctggaac cggggatggt
    49801 actgaccgta gagcctggtc tgtatatcgc gccggatgcg gatgtgccag aacaatatcg
    49861 cggtatcggc attcgaattg aagacgacat tgtgattacc gaaaccggta acgaaaacct
    49921 caccgccagc gtggtgaaaa agccggaaga aatcgaagcg ttgatggctg ctgcgagaaa
    49981 gcaatgagcg taatcatcgt cggtggcggc atggcgggcg cgacgctggc gctggctatt
    50041 tcccggttaa gtcacggggc gctgccggta catttgattg aagcgactgc gccagagtca
    50101 catgctcatc cgggctttga tggacgagcg atagcgctgg cggcgggtac ctgtcagcaa
    50161 ctggcgcgca tcggcgtctg gcaatctctg gcggattgcg caactgccat caccaccgtg
    50221 catgtcagcg atcgtggtca cgctggattt gtcaccctcg ccgcagaaga ttaccaactg
    50281 gcggcgctgg gacaggttgt cgaattgcac aatgtcgggc aacggctgtt tgcattgctg
    50341 cgtaaagcac ctggcgtaat gctgcattgc cccgatcgcg tagctaacgt tgcccgtact
    50401 cagagtcacg ttgaagtgac gctggagagt ggcgagacgc tgacgggccg cgtgctgata
    50461 gccgctgatg gcacccattc agcgttagcc accgcgtgcg gcgttgactg gcagcaggag
    50521 ccttacgaac aactggccgt gattgccaac gttgcgactt ccgttgcgca cgaaggccgc
    50581 gcttttgaac gcttcacgca acatggcccg ctggcgatgt tgccgatgtc tgacggacgc
    50641 tgttcgctgg tctggtgtca tccactggaa cggcgcgaag aggtgctgtc gtggagcgac
    50701 gagaagtttt gccgtgaact ccagtcggcc tttggctggc gacttggaaa aattacccac
    50761 gccggtaaac gcagtgctta tccgctggcg ttaacccgcg ccgccagagc tattacccat
    50821 cgtaccgtgc tggtgggcaa tgcggcgcaa actctgcacc cgattgccgg gcaagggttt
    50881 aacctcggta tgcgagatgt catgagcctt gcggaaaccc tgactcaggc gcatgagcgc
    50941 ggagaagaca tcggtgatta cggcatattg tgccgctatc agcagcgtcg acagagcgat
    51001 cgcgaagcaa ccatcggcgt cacggacagc ctggtacatc tttttgccaa ccgttgggca
    51061 ccgctggttg tcgggcgcaa catcggactg atgacgatgg aattattcac cccggcacgc
    51121 gatgtgctgg cgcagcgcac cctcggttgg gtggcgcgtt gaaggcggtt ttaaggaata
    51181 aacaatgcaa agtgttgatg tagccattgt tggtggcggc atggtggggc tggcggttgc
    51241 ctgcggctta caggggagcg gcttacgcgt tgccgtactg gagcagcgcg taccggaacc
    51301 tctggtggcg gatgcaccac cacaactgcg cgtttcggct atcaatgccg ccagcgaaaa
    51361 attactcacc cgtcttggcg tctggcagga cattctctct cgtagggcca gttgttatca
    51421 cggtatggaa gtgtgggaca aagacagttt tggtcacatt tcgtttgacg atcaaagcat
    51481 gggctatagc catcttgggc atatcgttga aaactcagtg attcactacg cgctgtggaa
    51541 caaagcgcag cagtcgtcag atatcactct gttggccccc gcagaattac agcaggtcgc
    51601 ctggggagaa aatgaaactt tcctgacgct gaaagatggc agcatgttaa cggcgcgtct
    51661 ggtgattggc gcggacggtg ctaattcctg gttgcgcaac aaagcagata ttccgctgac
    51721 tttctgggat tatcagcatc acgcgctggt agcgaccatt cgcacggaag aaccgcatga
    51781 tgcggtggcg cggcaggttt tccatggcga aggcattctg gcctttttac cgcttagcga
    51841 tccgcatctt tgctcaattg tctggtcact gtcgccagag gaagcgcagc ggatgcagca
    51901 ggcaagtgaa gatgaattta atcgcgcgtt aaatatcgct tttgataatc gcctgggctt
    51961 atgcaaggtt gagagcgcgc gtcaggtgtt cccactgacg gggcgttatg cgcgccagtt
    52021 tgccgcgcac cgtctggtgc tggtgggcga cgccgcgcat accattcacc cgctggcggg
    52081 gcagggagta aatctcggct ttatggatgc tgcagagctg attgctgaac tgaaacggtt
    52141 gcatcgtcag gggaaagaca tcgggcagta catttacctg cgccggtatg agcgtagccg
    52201 caagcacagt gcggcgctga tgctggctgg tatgcaggga ttccgcgatc tgttttccgg
    52261 taccaatccg gcgaaaaaac tgctgcgtga tattggtttg aaactggccg acacgcttcc
    52321 tggcgttaaa ccacaactta tccgccaggc aatgggatta aacgatttgc ctgaatggct
    52381 gcgttaaaaa tttctcttct gctgtttatt tgatacccat cacactttca tctcccggtt
    52441 ttttcgccgg gagattttcc tcatttgaaa taaactaatt tcacctccgt tttcgcatta
    52501 tattttctaa tgtcattatt ttttgattta gtgttttttg acattttttt agctcttaat
    52561 attgccttat tcaaattagc tttctaatgg catcatcttt atatagaaac tggtgtgttt
    52621 tttggttttt tattctgtcg cgattttggc gttttttaac cataagctaa tgtgatgatc
    52681 aattttacct tatggttaac agtctgtttc ggtggtaagt tcaggcaaaa gagaacgatt
    52741 gcgttgggga ccgggagtgg ctccgatgct gggtttcgtg gtgataattt caccatgaaa
    52801 agttgtcagc cccgcttatt caatgaggac aagatggcac aacagactcc tttgtacgaa
    52861 caacacacgc tttgcggcgc tcgcatggtg gatttccacg gctggatgat gccgctacat
    52921 tacggttcgc aaatcgacga acatcatgcg gtacgtaccg atgccggaat gtttgatgtg
    52981 tcacatatga ccatcgtcga tctccgcggc agccgcaccc gggagtttct gcgttatctg
    53041 ctggcgaacg atgtggcgaa gctcaccaaa agcggcaaag ccctttactc ggggatgttg
    53101 aatgcctctg gcggtgtgat agatgacctc atcgtctact actttactga agatttcttc
    53161 cgcctcgttg ttaactccgc cacccgcgaa aaagacctct cctggattac ccaacacgct
    53221 gaacctttcg gcatcgaaat taccgttcgt gatgaccttt ccatgattgc cgtgcaaggg
    53281 ccgaatgcgc aggcaaaagc tgccacactg tttaatgacg cccagcgtca ggcggtggaa
    53341 gggatgaaac cgttctttgg cgtgcaggcg ggcgatctgt ttattgccac cactggttat
    53401 accggtgaag cgggctatga aattgcgctg cccaatgaaa aagcggccga tttctggcgt
    53461 gcgctggtgg aagcgggtgt taagccatgt ggcttgggcg cgcgtgacac gctgcgtctg
    53521 gaagcgggca tgaatcttta tggtcaggag atggacgaaa ctatttctcc tttagccgcc
    53581 aacatgggct ggactatcgc ctgggaaccg gcagatcgtg actttatcgg tcgtgaagcc
    53641 ctggaagtgc agcgtgagca tggtacagaa aaactggttg gtctggtgat gaccgaaaaa
    53701 ggcgtgctgc gtaatgaact gccggtacgt tttaccgatg cgcagggcaa ccagcatgaa
    53761 ggcattatca ccagcgggac tttctccccg acgctgggtt acagcattgc gctggcacgc
    53821 gtgccggaag gtattggcga aacggcgatt gtgcaaattc gcaaccgtga aatgccggtt
    53881 aaagtgacga aacctgtttt tgtgcgtaac ggtaaagccg tcgcgtgatt tacttttttg
    53941 gagattgatt gatgagcaac gtaccagcag aactgaaata cagcaaagaa cacgaatggc
    54001 tgcgtaaaga agccgacggc actttcaccg ttggtataac cgaacatgcc caggagctgt
    54061 taggcgatat ggtgtttgtt gacctgccgg aagtgggcgc aacggttagc gcgggcgatg
    54121 actgcgcggt tgccgaatca gtaaaagcgg cgtcagacat ttatgcgcca gtaagcggtg
    54181 aaatcgtggc ggtaaacgac gcactgagcg attccccgga actggtgaac agcgaaccgt
    54241 atgcaggtgg ctggatcttt aaaatcaaag ccagcgatga aagcgaactg gaatcactgc
    54301 tggatgcgac cgcatacgaa gcattgttag aagacgagta acggctttat tcctcttctg
    54361 cgggagagga tcagggtgag gaaaatttat gcctcaccct cactctcttc gtaaggagag
    54421 aggttcacaa ttcactgcac gtttcaggaa ccatcgctca tgacacagac gttaagccag
    54481 cttgaaaaca gcggcgcttt tattgaacgc catatcggac cggacgccgc gcaacagcaa
    54541 gaaatgctga atgccgttgg tgcacaatcg ttaaacgcgc tgaccggcca gattgtgccg
    54601 aaagatattc agcttgcgac cccgccgcag gttggcgcac cggcgaccga atacgccgca
    54661 ctggcagaac tcaaggctat tgccagtcgc aataaacgct tcacgtctta catcggcatg
    54721 ggttacaccg ccgtgcagct accgccggtt atcctgcgta acatgctgga aaatccgggc
    54781 tggtataccg cgtacactcc gtatcaacct gaagtctccc agggccgcct tgaagcactg
    54841 ctcaacttcc agcaggtaac gctggatttg actggactgg atatggcctc tgcttctctt
    54901 ctggacgagg ccaccgctgc cgccgaagca atggcgatgg cgaaacgcgt cagcaaactg
    54961 aaaaatgcca accgcttctt cgtggcttcc gatgtgcatc cgcaaacgct ggatgtggtc
    55021 cgtactcgtg ccgaaacctt tggttttgaa gtgattgtcg atgacgcgca aaaagtgctc
    55081 gaccatcagg acgtcttcgg cgtgctgtta cagcaggttg gcacaaccgg tgaaatccac
    55141 gactacaccg cgcttattag cgaactgaaa tcacgcaaaa ttgtggtcag tgtcgccgcc
    55201 gatattatgg cgctggtgct gttaactgcg ccgggtaaac agggcgcgga tattgttttt
    55261 ggttcggcgc aacgcttcgg cgtgccgatg ggctacggtg gcccacacgc ggccttcttt
    55321 gcggcgaaag atgaatacaa acgctcaatg ccgggccgta ttatcggtgt atcgaaagat
    55381 gcagctggca ataccgcgct gcgcatggcg atgcagactc gcgagcaaca tattcgccgt
    55441 gagaaagcga actccaacat ttgtacttcc caggtgctgc tggcaaacat cgccagcctg
    55501 tatgccgttt atcacggccc gattggcctg aaacgtatcg ctaaccgcat tcaccgtctg
    55561 accgatatcc tggcggcggg cctgcaacaa aaaggtctga aactgcgcca tgcgcactat
    55621 ttcgacacct tgtgtgtgga agtggccgac aaagcgggcg tactgacgcg tgccgaagcg
    55681 gctgaaatca acctgcgtag cgatattctg aacgcggttg ggatcaccct tgatgaaaca
    55741 accacgcgtg aaaacgtaat gcagcttttc aacgtgctgc tgggcgataa ccacggcctg
    55801 gacatcgaca cgctggacaa agacgtggct cacgacagcc gctctatcca gcctgcgatg
    55861 ctgcgcgacg acgaaatcct cacccatccg gtgtttaatc gctaccacag cgaaaccgaa
    55921 atgatgcgct atatgcactc gctggagcgt aaagatctgg cgctgaatca ggcgatgatc
    55981 ccgctgggtt cctgcaccat gaaactgaac gccgccgccg agatgatccc aatcacctgg
    56041 ccggaatttg ccgaactgca cccgttttgt ccgccggaac aggctgaagg ttatcagcag
    56101 atgattgcgc agctggctga ctggctggtg aaactgaccg gttacgacgc cgtttgtatg
    56161 cagccgaact ccggcgcaca gggcgaatac gcgggcctgc tggcgattcg tcattatcat
    56221 gaaagccgca acgaagggca tcgcgatatc tgcctgatcc cggcttctgc gcacggaact
    56281 aaccccgctt ctgcacatat ggcaggaatg caggtggtgg ttgtggcgtg tgataaaaac
    56341 ggcaacatcg atctgactga tctgcgcgcg aaagcggaac aggcgggcga taacctctcc
    56401 tgtatcatgg tgacttatcc ttctacccac ggcgtgtatg aagaaacgat ccgtgaagtg
    56461 tgtgaagtcg tgcatcagtt cggcggtcag gtttaccttg atggcgcgaa catgaacgcc
    56521 caggttggca tcacctcgcc gggctttatt ggcgcggacg tttcgcacct caacctgcat
    56581 aaaactttct gcattccgca cggcggtggt ggtccgggta tgggaccgat cggtgtgaaa
    56641 gcgcatctgg caccgtttgt accgggtcat agcgtggtgc aaatcgaagg catgttaacc
    56701 cgtcagggcg cagtttctgc ggcaccgttc ggtagcgcct ctatcctgcc aatcagctgg
    56761 atgtacatcc gcatgatggg cgcagaaggg ctgaaaaaag caagccaggt ggcaatcctc
    56821 aacgccaact atattgccag ccgcctgcag gatgccttcc cggtgctgta taccggtcgc
    56881 gacggtcgcg tggcgcacga atgtattctc gatattcgcc cgctgaaaga agaaaccggc
    56941 atcagcgagc tggatattgc caagcgcctg atcgactacg gtttccacgc gccgaccatg
    57001 tcgttcccgg tggcgggtac gctgatggtt gaaccgactg aatctgaaag caaagtggaa
    57061 ctggatcgct ttatcgacgc gatgctggct atccgcgcag aaatcgacca ggttaaagcc
    57121 ggtgtctggc cgctggaaga taacccgctg gtgaacgcgc cgcacattca gaacgaactg
    57181 gtcgccgagt gggcgcatcc gtacagccgt gaggttgcag tattcccggc aggtgtggca
    57241 gacaaatact ggccgacggt gaaacgtctg gatgatgttt acggcgaccg taacctgttc
    57301 tgctcctgcg taccgattag cgaataccag taattcactg attcgactat tttctaaagg
    57361 cgcttcggcg cctttttagt cagatgacaa agtacaaaag tgctcagaca gtcccctcgc
    57421 ccctttgggg agagggtgag gggaacaggc cggcactggc gcgaatattt accctcaccc
    57481 cggccctctc cctgaaaggg cgagggggaa aagcgtgcca acattgaaga ttgagccagt
    57541 ttgttagcaa tctcaaagat acgtcaacga atgaattttt ctcggaaaaa caaatggtta
    57601 tagcacttgt gaccggtggc agtcgcggca tcgggcgggc aactgcatta ctgttggcgc
    57661 aagaagggta tacggtggcg gttaattatc agcaaaacct ccacgcggcg caggaagtga
    57721 tgaacttaat aacgcaagcc ggtggcaaag cattcgtgct ccaggcggat atcagcgacg
    57781 aaaaccaggt cgttgcgatg tttacagcaa tcgatcagca cgatgaaccg ctagcagcgc
    57841 tggtcaataa cgccgggatc ttgtttaccc agtgcaccgt tgaaaacctt accgcagagc
    57901 gaatcaaccg agtactttcc accaacgtga cgggatattt tctctgctgc cgcgaggcgg
    57961 taaaacgcat ggcgcttaaa aatggtggca gtggcggcgc tatcgtcaat gtctcttcgg
    58021 tggcctcacg gttgggttcg ccaggggaat atgttgatta cgcggcatcg aaaggggcga
    58081 ttgatacgtt aaccaccgga ctatcgctgg aagtcgccgc gcaggggatc cgcgttaact
    58141 gcgtgcggcc agggtttatt tataccgaaa tgcacgccag cggcggcgag cctggacgcg
    58201 tcgatcgcgt taagtcgaac atccccatgc agcgtggtgg acaggcagaa gaggtcgcgc
    58261 aggccattgt ctggctacta agtgataaag cctcttacgt cacgggaagt tttatcgatt
    58321 tggcgggcgg gaaataaaac agggaagttg tctgaccgga tgcaacaagt attgcatccg
    58381 gtacttcatc gacttaaagc ttctcgccgt tgctggcaat cacctcttgg taccagttaa
    58441 agctcttctt acgtgaacgc gacatatcgc cagcaccgtc gtcatgttta ttcacataga
    58501 taaagccgta gcgtttgctg tactgcccgg tggtgaacga tacgcagtcg atgcagcccc
    58561 acggtgtgta gcccatcaga tccacgccat cgtaagtcac cgcttttttc atctcttcga
    58621 tatgggcgcg caggtagtca atgcggtagt cgtcgttgat gctgccatct tcttccactt
    58681 tgtcgtaagc gccaaaaccg ttttcgacaa taaacagcgg cctctgataa cgctcataca
    58741 gttcgcaaag ggcatagcgc agacctactg gatcaatctg ccagccccag tcagacgctt
    58801 taacatacgg gttcggtacg ctgccttcga aaccagagat cgcatcgccg gtgccgcctt
    58861 cggccttcac tgcgttggtc atgtaatagc tgaaaccaag ataatcgcag gtgccttcac
    58921 gcagtacatc gagatcgccg tcttccattt tgatgttaaa tccgcgacgc tcccactcgt
    58981 tcaacacata ggacgggtaa tagccgcgta gctgcacatc ggtaaagacg tagcgttcgc
    59041 gcatcgactc ctgagcgaac atcacatcgt ccgggttaca gcagtaggga tagagcggca
    59101 ccatcgccag catacagccg actttcatct ccgggttaat gcgacgcgca gctttcaccg
    59161 ccagggcgct ggcgacaaac tggtgatgca gcacctggta catcgtctct tccgggttgt
    59221 catgctcggt ataaaccacg ccggagcagc agtaaccgaa cagcggtgca cgccagttac
    59281 gctggttgtt aatttcgttg aaggtcatcc agtatttgac tttatgctta tagcgttcaa
    59341 acaccacttc cgcgaaacgg acaaagaaat caaccacttt acggttggtc cagctaccgt
    59401 attgctgcac cagatgcagc ggcatttcaa agtgggagag ggtgatcacc ggttcgatgt
    59461 tgtatttcag cagttcatcg aacatatcat cgtagaactt cagcccttct tcgtttggct
    59521 gagcttcatc accttttgga aaaatacgcg tccaggcgat ggaagtccga aaacatttga
    59581 agcccatttc ggcaaatagc ttgatgtctt ccttgtagtg accataaaaa tcaacggctt
    59641 catggtttgg atagtatttg cctggcaaga cttctttggt gatttcgcgc ggcacgccgt
    59701 gtgcgccacc ggtcagaacg tcacaaatgc tcggcccttt gccgcctttg ttccagctgc
    59761 cttcgacctg atgagcggca actgcgccgc cccataagaa atctttcggt aaggtgagtt
    59821 ttttcactat cataggctcc agatgagtca attcttacag gaaatagtac catgcagcca
    59881 aacgacatca ctttttttca acgtttccag gatgacattc tggctgggcg taaaaccatc
    59941 accatccgcg acgagtctga atcgcacttc aaaacgggtg atgtgcttcg tgtcgggcgt
    60001 tttgaagatg acggttattt ttgcacgatt gaagtcaccg caacctcaac cgtaaggctg
    60061 gatacgctga ctgaaaaaca tgcagagcag gaaaatatga ccctgactga actgaaaaag
    60121 gtcattgccg acatctatcc cggtcagaca cagttttatg tgattgaatt taaatgtctt
    60181 taaattgaac tcacgcctgt tagttgaaat ttctcattta atttcatgat ttttaggaga
    60241 ttagtctcaa atcagaattt attttagcta acaggtgttc actggaacta ttctcagtta
    60301 cgctggagag gtatacatgt gcgtgtacgt ttctccggag taagttatgg ttcagaagcc
    60361 cctcattaag cagggatatt cgctggcaga ggaaattgcc aacagcgtca gtcacggcat
    60421 tgggttggtg tttggtatcg ttgggctggt gttgctactg gttcaggcgg tggatctcaa
    60481 tgccagcgcc acagcgataa ccagctacag cctctatggc ggcagtatga tcctgctgtt
    60541 cctcgcttcg acgctctatc acgccattcc tcatcaacgg gcaaaaatgt ggctgaagaa
    60601 atttgaccat tgcgctattt acctgttgat tgccggaacc tacacgccgt ttttgctggt
    60661 ggggctggat tctccgttag cgcgcgggtt gatgattgtt atctggagcc tggcattgct
    60721 ggggattctg tttaaactga ccatcgcgca ccgattcaaa attttatctc tggtgaccta
    60781 tctggcgatg ggctggctgt cgctggtggt aatttatgaa atggcagtta agctcgcggc
    60841 gggcagcgtt accttactgg cggtaggcgg cgtggtttat tcgctcgggg tgattttcta
    60901 cgtctgcaaa cgcattccat acaaccatgc catctggcac ggcttcgtgc tcggcggtag
    60961 tgtgtgccac tttctggcga tctatttgta tattgggcag gcgtaatggg tatgcctt
//
LOCUS       DAZFXT010000014        53550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.014, whole genome shotgun sequence.
ACCESSION   DAZFXT010000014 DAZFXT010000000
VERSION     DAZFXT010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.014"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            322..1686
                     /gene="yajR"
                     /locus_tag="ACHKW0_000981"
     CDS             322..1686
                     /gene="yajR"
                     /locus_tag="ACHKW0_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7473859.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1814..2305)
                     /gene="yajQ"
                     /locus_tag="ACHKW0_000982"
     CDS             complement(1814..2305)
                     /gene="yajQ"
                     /locus_tag="ACHKW0_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HFO7473860.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2473..3384
                     /gene="panE"
                     /locus_tag="ACHKW0_000983"
     CDS             2473..3384
                     /gene="panE"
                     /locus_tag="ACHKW0_000983"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HFO7473861.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3347..3937
                     /gene="yajL"
                     /locus_tag="ACHKW0_000984"
     CDS             3347..3937
                     /gene="yajL"
                     /locus_tag="ACHKW0_000984"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HFO7473862.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVANGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3991..5439)
                     /gene="thiI"
                     /locus_tag="ACHKW0_000985"
     CDS             complement(3991..5439)
                     /gene="thiI"
                     /locus_tag="ACHKW0_000985"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HFO7473863.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5645..5887
                     /gene="xseB"
                     /locus_tag="ACHKW0_000986"
     CDS             5645..5887
                     /gene="xseB"
                     /locus_tag="ACHKW0_000986"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HFO7473864.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5887..6786
                     /gene="ispA"
                     /locus_tag="ACHKW0_000987"
     CDS             5887..6786
                     /gene="ispA"
                     /locus_tag="ACHKW0_000987"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HFO7473865.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6811..8673
                     /gene="dxs"
                     /locus_tag="ACHKW0_000988"
     CDS             6811..8673
                     /gene="dxs"
                     /locus_tag="ACHKW0_000988"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HFO7473866.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPEAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8853..9827
                     /gene="yajO"
                     /locus_tag="ACHKW0_000989"
     CDS             8853..9827
                     /gene="yajO"
                     /locus_tag="ACHKW0_000989"
                     /EC_number="1.17.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HFO7473867.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGRNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9881..10399)
                     /gene="pgpA"
                     /locus_tag="ACHKW0_000990"
     CDS             complement(9881..10399)
                     /gene="pgpA"
                     /locus_tag="ACHKW0_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HFO7473868.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10377..11354)
                     /gene="thiL"
                     /locus_tag="ACHKW0_000991"
     CDS             complement(10377..11354)
                     /gene="thiL"
                     /locus_tag="ACHKW0_000991"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HFO7473869.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11432..11851)
                     /gene="nusB"
                     /locus_tag="ACHKW0_000992"
     CDS             complement(11432..11851)
                     /gene="nusB"
                     /locus_tag="ACHKW0_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HFO7473870.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11871..12341)
                     /gene="ribE"
                     /locus_tag="ACHKW0_000993"
     CDS             complement(11871..12341)
                     /gene="ribE"
                     /locus_tag="ACHKW0_000993"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HFO7473871.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12430..13533)
                     /gene="ribD"
                     /locus_tag="ACHKW0_000994"
     CDS             complement(12430..13533)
                     /gene="ribD"
                     /locus_tag="ACHKW0_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HFO7473872.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSTQARRDVQRLRAQSHAILTSSATVLADDPALTVRWSELDEQTQVL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQVGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13537..13986)
                     /gene="nrdR"
                     /locus_tag="ACHKW0_000995"
     CDS             complement(13537..13986)
                     /gene="nrdR"
                     /locus_tag="ACHKW0_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HFO7473873.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            14137..14676
                     /gene="yajI"
                     /locus_tag="ACHKW0_000996"
     CDS             14137..14676
                     /gene="yajI"
                     /locus_tag="ACHKW0_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO7473874.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSALNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLKSQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14975..15859
                     /gene="tsx"
                     /locus_tag="ACHKW0_000997"
     CDS             14975..15859
                     /gene="tsx"
                     /locus_tag="ACHKW0_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HFO7473875.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(16036..16383)
                     /gene="yajD"
                     /locus_tag="ACHKW0_000998"
     CDS             complement(16036..16383)
                     /gene="yajD"
                     /locus_tag="ACHKW0_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease YajD"
                     /protein_id="HFO7473876.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16512..17483)
                     /gene="secF"
                     /locus_tag="ACHKW0_000999"
     CDS             complement(16512..17483)
                     /gene="secF"
                     /locus_tag="ACHKW0_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HFO7473877.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17494..19341)
                     /gene="secD"
                     /locus_tag="ACHKW0_001000"
     CDS             complement(17494..19341)
                     /gene="secD"
                     /locus_tag="ACHKW0_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HFO7473878.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19369..19701)
                     /gene="yajC"
                     /locus_tag="ACHKW0_001001"
     CDS             complement(19369..19701)
                     /gene="yajC"
                     /locus_tag="ACHKW0_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HFO7473879.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19724..20851)
                     /gene="tgt"
                     /locus_tag="ACHKW0_001002"
     CDS             complement(19724..20851)
                     /gene="tgt"
                     /locus_tag="ACHKW0_001002"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HFO7473880.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20907..21977)
                     /gene="queA"
                     /locus_tag="ACHKW0_001003"
     CDS             complement(20907..21977)
                     /gene="queA"
                     /locus_tag="ACHKW0_001003"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051075 - S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HFO7473881.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            22070..22651
                     /gene="acpH"
                     /locus_tag="ACHKW0_001004"
     CDS             22070..22651
                     /gene="acpH"
                     /locus_tag="ACHKW0_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HFO7473882.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22656..24473)
                     /gene="malZ"
                     /locus_tag="ACHKW0_001005"
     CDS             complement(22656..24473)
                     /gene="malZ"
                     /locus_tag="ACHKW0_001005"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308480.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004558 - alpha-1,4-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HFO7473883.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPFLNVAQWQRKEGHGQLTNGILALPAISATVWMN"
     gene            complement(24629..26002)
                     /gene="proY"
                     /locus_tag="ACHKW0_001006"
     CDS             complement(24629..26002)
                     /gene="proY"
                     /locus_tag="ACHKW0_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HFO7473884.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(26078..27397)
                     /gene="brnQ"
                     /locus_tag="ACHKW0_001007"
     CDS             complement(26078..27397)
                     /gene="brnQ"
                     /locus_tag="ACHKW0_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HFO7473885.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27804..29099)
                     /gene="phoR"
                     /locus_tag="ACHKW0_001008"
     CDS             complement(27804..29099)
                     /gene="phoR"
                     /locus_tag="ACHKW0_001008"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HFO7473886.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(29157..29846)
                     /gene="phoB"
                     /locus_tag="ACHKW0_001009"
     CDS             complement(29157..29846)
                     /gene="phoB"
                     /locus_tag="ACHKW0_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HFO7473887.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            30036..31238
                     /gene="sbcD"
                     /locus_tag="ACHKW0_001010"
     CDS             30036..31238
                     /gene="sbcD"
                     /locus_tag="ACHKW0_001010"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HFO7473888.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITADEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            31235..34378
                     /gene="sbcC"
                     /locus_tag="ACHKW0_001011"
     CDS             31235..34378
                     /gene="sbcC"
                     /locus_tag="ACHKW0_001011"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HFO7473889.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHTRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQHLVALHGQIVPQQKRLAQLQVAIQ
                     NVTQEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34504..35688
                     /gene="araJ"
                     /locus_tag="ACHKW0_001012"
     CDS             34504..35688
                     /gene="araJ"
                     /locus_tag="ACHKW0_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HFO7473890.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNVLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35832..36740)
                     /gene="mak"
                     /locus_tag="ACHKW0_001013"
     CDS             complement(35832..36740)
                     /gene="mak"
                     /locus_tag="ACHKW0_001013"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HFO7473891.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36865..37776
                     /gene="rdgC"
                     /locus_tag="ACHKW0_001014"
     CDS             36865..37776
                     /gene="rdgC"
                     /locus_tag="ACHKW0_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HFO7473892.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37854..37937)
                     /locus_tag="ACHKW0_001015"
     CDS             complement(37854..37937)
                     /locus_tag="ACHKW0_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473893.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37934..38275)
                     /locus_tag="ACHKW0_001016"
                     /pseudo
     CDS             complement(37934..38275)
                     /locus_tag="ACHKW0_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38423..38707)
                     /gene="ppnP"
                     /locus_tag="ACHKW0_001017"
     CDS             complement(38423..38707)
                     /gene="ppnP"
                     /locus_tag="ACHKW0_001017"
                     /EC_number="2.4.2.1"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HFO7473894.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(38779..39456)
                     /gene="aroM"
                     /locus_tag="ACHKW0_001018"
     CDS             complement(38779..39456)
                     /gene="aroM"
                     /locus_tag="ACHKW0_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HFO7473895.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDIQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39714..39905)
                     /gene="yaiA"
                     /locus_tag="ACHKW0_001019"
     CDS             complement(39714..39905)
                     /gene="yaiA"
                     /locus_tag="ACHKW0_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HFO7473896.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39955..40479)
                     /gene="aroL"
                     /locus_tag="ACHKW0_001020"
     CDS             complement(39955..40479)
                     /gene="aroL"
                     /locus_tag="ACHKW0_001020"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HFO7473897.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(40662..41120)
                     /gene="yaiI"
                     /locus_tag="ACHKW0_001021"
     CDS             complement(40662..41120)
                     /gene="yaiI"
                     /locus_tag="ACHKW0_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HFO7473898.1"
                     /translation="MTIWVDADACPSVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            41240..42049
                     /gene="proC"
                     /locus_tag="ACHKW0_001022"
     CDS             41240..42049
                     /gene="proC"
                     /locus_tag="ACHKW0_001022"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HFO7473899.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(42066..43181)
                     /gene="adrA"
                     /locus_tag="ACHKW0_001023"
                     /gene_synonym="dgcC"
     CDS             complement(42066..43181)
                     /gene="adrA"
                     /locus_tag="ACHKW0_001023"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HFO7473900.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(43283..43603)
                     /gene="psiF"
                     /locus_tag="ACHKW0_001024"
     CDS             complement(43283..43603)
                     /gene="psiF"
                     /locus_tag="ACHKW0_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HFO7473901.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAEKTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(43722..45137)
                     /gene="phoA"
                     /locus_tag="ACHKW0_001025"
     CDS             complement(43722..45137)
                     /gene="phoA"
                     /locus_tag="ACHKW0_001025"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HFO7473902.1"
                     /translation="MKQSTIALALLTLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDHTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(45238..45498)
                     /gene="iraP"
                     /locus_tag="ACHKW0_001026"
     CDS             complement(45238..45498)
                     /gene="iraP"
                     /locus_tag="ACHKW0_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HFO7473903.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
     gene            complement(45680..45883)
                     /locus_tag="ACHKW0_001027"
     CDS             complement(45680..45883)
                     /locus_tag="ACHKW0_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7473904.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFSLNYKLTKRIYFITFIYNKG"
     gene            45961..47055
                     /gene="ddlA"
                     /locus_tag="ACHKW0_001028"
     CDS             45961..47055
                     /gene="ddlA"
                     /locus_tag="ACHKW0_001028"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HFO7473905.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(47079..47291)
                     /gene="yaiZ"
                     /locus_tag="ACHKW0_001029"
     CDS             complement(47079..47291)
                     /gene="yaiZ"
                     /locus_tag="ACHKW0_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HFO7473906.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            47551..47859
                     /gene="yaiY"
                     /locus_tag="ACHKW0_001030"
     CDS             47551..47859
                     /gene="yaiY"
                     /locus_tag="ACHKW0_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HFO7473907.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(47918..49012)
                     /gene="yaiW"
                     /locus_tag="ACHKW0_001031"
     CDS             complement(47918..49012)
                     /gene="yaiW"
                     /locus_tag="ACHKW0_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HFO7473908.1"
                     /translation="MSRVNPLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(49025..50245)
                     /gene="sbmA"
                     /locus_tag="ACHKW0_001032"
     CDS             complement(49025..50245)
                     /gene="sbmA"
                     /locus_tag="ACHKW0_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HFO7473909.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGEWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSTHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            50597..51754
                     /gene="ampH"
                     /locus_tag="ACHKW0_001033"
     CDS             50597..51754
                     /gene="ampH"
                     /locus_tag="ACHKW0_001033"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HFO7473910.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(51755..52378)
                     /gene="iprA"
                     /locus_tag="ACHKW0_001034"
     CDS             complement(51755..52378)
                     /gene="iprA"
                     /locus_tag="ACHKW0_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HFO7473911.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(52466..>53550)
                     /gene="ehaB"
                     /locus_tag="ACHKW0_001035"
     CDS             complement(52466..>53550)
                     /gene="ehaB"
                     /locus_tag="ACHKW0_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HFO7473912.1"
                     /translation="RIDNATGAGAIADYKDKEIIYVNDVNTNATFSAANKADLGAYTY
                     QAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNGGA
                     WVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDRSG
                     QVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWGFG
                     LKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAGYT
                     FTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSA
                     YTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT        13295 a        14212 c        13379 g        12664 t
ORIGIN      
        1 ggtccaagta ataagcacac atttagcctg aaacattatc atccatgtca ccaaatcgtt
       61 tatttagtga gataaaacta gcatattcgt aagataaaaa atgcacatgg caatcaagaa
      121 atgatattat tactttgttg atgtaaaact atttagtttt aaaattcatt ttttcaacat
      181 atagagcatt cattttatga atgcaaaaaa acatacctga cacacaaatt actctcctct
      241 gtaatatttt ctcattaaca ctctgtttac ccaacatgcg ctccgcacta cactatgtgg
      301 gcattttttt aaggggtggt aatgaacgat tataaaatga cgccaggtga gaggcgcgcg
      361 acctggggtt tagggaccgt attctcgttg cgcatgctgg gcatgtttat ggttctgccg
      421 gttctgacca cgtacggcat ggctctgcaa ggcgccagcg aagcattaat cggtattgcc
      481 attggtattt atggtctgac tcaggccgtt tttcagattc cgtttggcct gctttccgac
      541 cgcattggtc gcaaaccatt aattgtcggt gggctggcgg tgtttgccgc cggtagcgtt
      601 atcgctgcgc tttccgactc catctgggga attattctgg gccgggcgct acaaggctct
      661 ggagcaattg ccgccgccgt tatggcgctg ctttccgatc tcacgcgcga acaaaaccgc
      721 accaaagcga tggcgtttat cggcgtgagc tttggcatta cctttgccat tgcgatggtg
      781 cttggcccga tcatcactca caaacttggg ctgcacgcgc tgttctggat gatcgctatt
      841 ctggcaacaa ccggcattgc gttgaccatt tgggttgtgc ccaacagtag cactcacgta
      901 cttaatcgtg agtccggaat ggtgaaaggc agtttcagta aagtgctggc ggaaccgcgg
      961 ctgctaaaac tcaactttgg cattatgtgt ctgcacatgc ttctgatgtc tacgtttgtt
     1021 gccctgcccg gacagctagc tgatgcgggg ttcccggcgg ctgaacactg gaaggtctat
     1081 ctggcgacga tgttaatcgc ctttggctcg gtcgtgcctt tcattatcta cgctgaagtt
     1141 aagcgcaaaa tgaagcaagt ctttgtcttc tgcgtcgggt tgatcgtggt tgcggaaatt
     1201 gtgttgtgga acgcacaaac gcagttctgg caactggtgg tcggcgtgca gcttttcttt
     1261 gtggcgttta atttgatgga agcccttctg ccctcactta tcagtaaaga gtcgccagca
     1321 ggttacaaag gtacggcgat gggtgtctac tccaccagcc agtttcttgg cgtggcgatt
     1381 ggcggttcgc tgggcggctg gattgacggc atgtttgacg gtcagggggt atttctcgct
     1441 ggcgcaatgc tggccgcagt gtggctggca gtcgccagta ccatgaaaga accgccgtat
     1501 gtcagcagtt tgcgcattga aatcccggcg gacattgccg caaacgaagc gttaaaagtg
     1561 cgtttgctgg aaactgaagg cgtcaaagaa gtgttgattg cagaagaaga gcattcagct
     1621 tatgtgaaaa tcgacagcaa agtgacaaat cgctttgagg ttgaacaggc aattcgtcag
     1681 gcataaaaaa cgcggcgaat tgcctgctgc gctacgctta ccaggcctac aaagttcttg
     1741 agttacaaac cgttgtaggc cgaatcaggc gttcatgccg catctgacat gaacaaaacg
     1801 cacattgtcg cgattaatcg cggaagtttt tgaactggaa cggctggccg agatcgccac
     1861 cacgtaccat cgccatgaca gcctgcaaat catcacgaga tttgccagtc acacggatct
     1921 catcgccctg aatttgcgcc tgcactttca gtttgctgtc tttaatcatc ttgacgattt
     1981 tcttctgggt cgcgctctca atgccctgtt tcagtttcgc ttccacaaac caggttttac
     2041 cgctatgaac gatattttcc ggtacatcca gtgaactgcc ttcaatgccg cgcttcagca
     2101 gcttggcacg cagaatatcc agcaactgat tgacctggaa gtcggactcg ctcaacactt
     2161 tgatggtttt gctggcgtcg ttcagctcaa atgaggcttc aacgttacgg aagtcaaaac
     2221 gggactccac ttcgcgactc gcgttatcga ccgcgttacg tgcttcctga agatcaactt
     2281 cagagacaat atcgaaagat ggcatctttt cctctccctt catttttgat gcgaagcata
     2341 atacctgcaa agttaaataa ccaacagtcc tgcaccagga cgtatccctt aattgtgcat
     2401 agtcgcaaca gcatgcacaa ttaagggata gccttattga acacccggag tggttgcggg
     2461 tgaggaggaa caatgaaaat taccgtattg ggatgcggtg ccttagggca attatggctt
     2521 acagcacttt gtaaacaggg tcacgaagtt cagggctggc tgcgcgtacc gcaaccttat
     2581 tgtagtgtga atctggttga gactgatggt tcgatattta acgaatcgct gaccgccaac
     2641 gatcccgatt ttcttgccac cagcgatctg ctcctggtga cgctgaaagc atggcaggtt
     2701 tacgatgccg tcaaaagcct cgcgtccaca ctgcctgtaa ccacgccaat actgttaatt
     2761 cacaacggca tgggcaccat cgaagagttg caaaacattc agcagccatt actgatgggc
     2821 accaccaccc atgccgcccg ccgcgacggc aatgtcatta ttcatgtggc aaacggaatc
     2881 acgcatattg gtccggcacg gcaacaggat ggcgattaca gttatctggc ggatattttg
     2941 caaaccgtgt tgcctgacgt tgcctggcat aacaatattc gcgccgagct gtggcgcaag
     3001 ctggccgtca actgtgtgat taatccactg accgccatct ggaattgccc gaatggtgaa
     3061 ttacgtcatc atccgcaaga aattatgcag atatgcgaag aagtcgcggc agtgatcgag
     3121 cgcgaagggc atcatacttc agcagaagat ttgcgtgatt acgtgatgca ggtgattgat
     3181 gccacagcgg aaaatatctc gtcgatgttg caggatatcc gcgcgctgcg ccacaccgaa
     3241 atcgactata tcaatggttt tctcttacgc cgcgcccgcg cgcatgggat tgccgtaccg
     3301 gaaaacaccc gcctgtttga aatggtaaaa agaaaggaga gtgaatatga gcgcatcggc
     3361 actggtttgc ctcgcccctg gtagtgaaga gactgaagcc gtcaccacta tcgatctgct
     3421 ggttcgcggc ggtatcaaag tcaccactgc cagcgtcgcc agcgatggca acctggccat
     3481 tacctgctcg cgcggcgtga agctgctggc ggatgcgccg ctggtcgagg tggctaatgg
     3541 cgaatatgac gtgatcgtac tgcctggtgg cattaaaggc gcagagtgtt ttcgcgatag
     3601 taccctgctg gttgaaaccg ttaaacagtt ccaccgttcc gggcgtatcg tcgcggctat
     3661 ttgcgccgcg ccagccaccg tgctggtacc gcacgatatc ttcccgattg gcaatatgac
     3721 cggcttcccg acgctgaaag acaaaattcc cgccgaacaa tggcaggaca agcgcgtcgt
     3781 ctgggatgca cgggtaaaat tgctgaccag ccaggggccg ggtacggcta ttgattttgg
     3841 tctgaaaatt atcgacctgc tggttgggcg cgagaaagcc catgaagtgg catcacaact
     3901 ggtgatggcg gcagggattt ataattatta cgagtagtgt cggatgcggc aaacgtcgca
     3961 tctgaccaga tgcgacgtaa aaaccacaaa ttatgggcga tacaccttca cattgttaaa
     4021 gccctgctcg cgcagataga gcgcctgcag acggctcatc accccgcgct cacaccacag
     4081 tagccaggtt ctgttctggt cgagatcgcc aaacttggtg ctcagtttat agaacggcag
     4141 agaaactaca tcaatccctt cgactttcag tggcttatct tcctgttcat cgatagaacg
     4201 gatatcgagg atcacgtcgt tcgggccgaa gccattgacg gtttccactt ccaccacttc
     4261 ctgctcggtc tgctgggcga tttcgcggat atcaacgtta ttcgcttctt caaccacttt
     4321 atcgagaatg ctgaagtcga atttctcttc ttccgcttca atcttcgact taaccgcttt
     4381 caccgtcggg cttttggaga taacaccgca atattccggc atcgtgcgag caaagtcttc
     4441 ggtgccaatc tggcgagcca ggttgatgat gtgctctttg tcgtaagaaa tcagcggacg
     4501 caggatcagc gtatcggaga cgttatcaat caggcgcagg ttggtcagcg tctgactgga
     4561 cacctggccg agcgcttcgc cggtgaccag cgcctgtacg ccgtaacgtt cagccacttt
     4621 agacgcggca cgcaccatca tacgtttgag gataacgccc atctgaccgt cgtcgatttt
     4681 ctcgagaatt tccccgacga ccggttcgaa attaatagcg acaaaacgca cgcggtggga
     4741 gctgccaaaa cggttccaca gataatgcgc cacctgacgc acgccaattt catgcgccgc
     4801 gccgccaagg ttaaagaaac agtaatgcac gcggcagccg cgacgcatca acatataact
     4861 ggaaacaccg gagtcgaaac caccggaaat gagcgacagc acatcttcct gggtgccgat
     4921 cgggaaaccg ccaatacctt cgtagcggcc tttaatcagc aggagacgat cgtcttccac
     4981 ttccagatgg acagtcactt ccggattggt cagcttcacg cgcgcggact caatatgctg
     5041 atttaaaccg ccgccgacgt aacgttccac atcaatcgag ctaaaatcat gtttgccacg
     5101 acgcttcacg cgtacgcaga aggttttgcc ttccagctga tcgcgatact gaaccaacgc
     5161 tttctcaaaa atatcgtgca tgtcggtaaa cggcacgtct tcgacttcga gaatatggtg
     5221 gatacccgga atacgggtca gggcgtcgcg aatagccaga cgctggtttt catcttttgc
     5281 gcgaacttcg atgttatccc agtggcggac gacagcgagc gtctcatcat agtgctttaa
     5341 aacgttacga atgttcccgg taaggatttt tataaagcgc aagcgcacag attggctttt
     5401 gatggtgatt tccgggaaca atttaatgat aaacttcatg gcggcaatgg ttcgttggca
     5461 agccttaagc gacttgtata gggaaaaata cagcagccca cacctgcggc tgcatccagg
     5521 cgcggaagta taccactaac atcgctttgc tgcgcacatc accttaccat tgcgcgttat
     5581 ttgctatttg ccctgagtcc gttaccatga cggggcgaaa aatattgaga gtcagacatt
     5641 cattatgccg aagaaaaatg aggctcccgc cagctttgaa aaggcgctga gcgagctgga
     5701 acagattgta acccgtctgg aaagtggcga cctgccgctg gaagaggcgc tgaacgagtt
     5761 cgaacgcggc gtgcagctgg cacgtcaggg gcaggccaaa ttacaacaag ccgaacagcg
     5821 cgtacaaatt ctgctgtctg acaatgaaga cgcctctcta acccctttta caccggacaa
     5881 tgagtaatgg actttccgca gcaactcgaa gcctgcgtta agcaggccaa ccaagcgctg
     5941 agccgtttta tcgccccact gccctttcag aacactcccg tggtcgaaac catgcagtat
     6001 ggcgcattat taggtggtaa gcgcctgcga ccttttctgg tttatgccac cggtcatatg
     6061 tttggcgtta gcacaaacac gctggacgca cccgctgccg ccgtagagtg tatccacgct
     6121 tactcattaa ttcatgatga tttaccggcg atggatgatg acgatctgcg tcgcggtttg
     6181 ccgacctgcc atgtgaagtt tggcgaagca aacgcgattc tcgctggcga cgctttacaa
     6241 acgctggcgt tctcgattct aagcgatgcc aatatgccgg aagtgtcgga tcgcgacaga
     6301 atttcgatga tttctgaact ggcgagcgcc agcggtattg ccggaatgtg cggtggtcag
     6361 gcattagatt tagacgcgga aggcaaacac gtacctctgg acgcgcttga gcgtattcat
     6421 cgtcataaaa ccggcgcatt gattcgcgcc gccgttcgcc ttggtgcatt aagcgccgga
     6481 gataaagggc gtcgtgctct gccagtactc gacaagtacg cagagagcat cggccttgcc
     6541 ttccaggttc aggatgacat cctggatgtg gtaggagata ctgcaacgtt gggaaaacgc
     6601 cagggtgccg accagcaact tggtaaaagt acctaccctg cacttctggg tcttgagcaa
     6661 gcccggaaga aagcccggga tctgatcgac gatgcccgcc agtcgctgaa acaactggct
     6721 gaacaatcac tcgatacctc ggcactggaa gcgctagcgg actacatcat ccagcgtaat
     6781 aaataaacaa taagtattaa taggcccctg atgagttttg atattgccaa atacccgacc
     6841 ctggcactgg tcgactccac ccaggagtta cgactgttgc cgaaagagag tttaccgaaa
     6901 ctctgcgacg aactgcgccg ctatttactc gacagcgtga gccgttccag cgggcacttc
     6961 gcctccgggc tgggcacggt cgaactgacc gtggcgctgc actatgtcta caacaccccg
     7021 tttgaccaat tgatttggga tgtggggcat caggcttatc cgcataaaat tttgaccgga
     7081 cgccgcgaca aaatcggcac catccgtcag aaaggcggcc tgcacccgtt cccgtggcgc
     7141 ggcgaaagcg aatatgacgt attaagcgtc ggtcattcat caacctccat cagtgccgga
     7201 attggtattg cggttgctgc cgagaaagaa ggcaaaaatc gccgcaccgt ctgtgtcatt
     7261 ggcgatggcg cgattaccgc tggcatggcg tttgaagcga tgaatcacgc gggcgatatc
     7321 cgtcctgata tgctggtggt cctcaacgac aatgaaatgt cgatttccga aaatgtcggc
     7381 gcgctcaata accatctggc acagctgctt tccggtaagc tttactcttc gctgcgcgaa
     7441 ggcgggaaaa aagttttctc tggcgttccg ccaattaaag agctgctcaa acgtaccgaa
     7501 gaacatatta aaggcatggt agtgcctggc acgttgtttg aagagctggg ctttaactac
     7561 atcggcccgg ttgacggtca cgatgtgctg gggcttatca ccacgctgaa gaacatgcgc
     7621 gacctgaaag gcccgcagtt cctgcatatc atgaccaaaa aaggtcgtgg ttatgaaccg
     7681 gcagaaaaag accccatcac tttccacgcc gtgcctaaat ttgatccctc cagcggttgt
     7741 ttgccgaaaa gtagcggcgg tttgccgagc tattcaaaaa tctttggcga ctggttgtgc
     7801 gaaacggcag cgaaagacaa caagctgatg gcgattactc cggcgatgcg tgaaggttcc
     7861 ggcatggtcg agttttcacg taaattcccg gatcgttact tcgacgtggc aatcgccgag
     7921 caacacgcgg tgacctttgc cgccggtctg gcgattggtg ggtacaaacc cattgtcgcg
     7981 atttactcca ctttcctgca acgcgcctat gatcaggtgc tgcatgacgt ggcgattcaa
     8041 aagctcccgg tcctgttcgc catcgaccgc gcgggcattg ttggtgctga cggtcaaacc
     8101 catcagggcg cttttgacct ctcttacctg cgctgcatac cggaaatggt cattatgacc
     8161 ccgagcgatg aaaacgaatg tcgccagatg ctctataccg gctatcacta taacgatggc
     8221 ccgtcagcgg tgcgctaccc gcgtggcaac gcggtcggcg tggaactgac gccgctggaa
     8281 aaactaccaa ttggcaaagg cattgtgaag cgtcgtggcg agaaactggc gatccttaac
     8341 tttggtacgc tgatgccaga agcggcgaaa gtcgccgaat cgctgaacgc cacgctggtc
     8401 gatatgcgtt ttgtgaaacc gcttgatgaa gcgttaattc tggaaatggc cgccagccat
     8461 gaagcgctgg tcaccgtaga agaaaacgcc attatgggcg gcgcaggcag cggcgtgaac
     8521 gaagtgctaa tggcccatcg taaaccagta cccgtgctga acattggcct gcctgacttc
     8581 tttattccgc aaggaactca ggaagaaatg cgcgccgaac tcggcctcga tgccgccggt
     8641 atggaagcca aaatcaaggc ctggctggca taatccctac tccactcctg ctatgcttaa
     8701 caaattattc atagactcta aataattcga gttgcaggaa ggcggcaaac gagtgaagcc
     8761 ccaggagctt acataagtaa gtgactgggg tgaacgaatg cagccgcagc acatgcaact
     8821 tgaagtatga cgagtatggc aggagtggca gcatgcaata caacccctta ggaaaaaccg
     8881 accttcgcgt ttcccgactt tgcctcggct gtatgacctt tggcgagcca gatcgtggta
     8941 atcacgcatg gacactgccg gaagagagta gccgtcccat cattaaacgc gcgctggaag
     9001 gcggcataaa tttctttgat accgccaata gctattccga tggcagcagc gaagagatcg
     9061 ttggtcgcgc actgcgggat ttcgcccgtc gtgaagacgt ggtcgttgcg acgaaagtgt
     9121 tccatcgcgt tggtgattta ccggaaggat tatcccgtgc acaaattttg cgctctatcg
     9181 acgacagcct gcgccgtctc ggcatggatt atgtcgatat cctgcaaatt catcgctggg
     9241 attacaacac gccgatcgaa gagacgctgg aagccctgaa cgacgtggta aaagccggga
     9301 aagcgcgtta tatcggcgcg tcatccatgc acgcttcgca gtttgctcag gccctgaagc
     9361 tacaaaaaca gcacggctgg gcgcagttta tcagtatgca ggatcactac aatctgattt
     9421 atcgcgaaga agagcgcgag atgctgcctc tgtgttatca ggagggcgtg gcggtgattc
     9481 cgtggagccc gctggcgcgg gggcgactga cgcgtccgtg gggagaaact accgcacgac
     9541 tggtgtctga tgaggtgggg agaaatctct ataaagaaag cgatgaaaat gacgcgcaga
     9601 tcgcagagcg gttaacgggc gtcagtgaag aactcggtgc aacacgagca caagttgcgc
     9661 tggcctggtt gttgagtaaa ccgggcattg ccgcaccgat tatcggtaca tcgcgggaag
     9721 aacagcttga tgagctgctg aacgcggtgg atatcacttt gaagccagaa cagattgccg
     9781 aactggaaac gccgtataaa ccgcatgcgg tcgtaggatt taaataagag tccaggcctg
     9841 atgagacgtg acaagcgtca catcaggcat cggtgcacaa ctacgacaga atacccagcg
     9901 gccagtgatg accgataaag tacaggatgc ctgcggaaat caccccggcg acaatatcgt
     9961 cgatcatgat ccccatgccg ccatgtacat tgcgatcaaa ccagcggatc ggccacggct
    10021 tccacatatc cagaatacgg aaaatcacaa acccggcggc aacccactgc cagtcattgg
    10081 tcggcagcgc catgagcgtg atccacatac caataaattc gtcccagaca atgctgccgt
    10141 gatcgtgcac gcccatatct ttcgccgttt gatgacaaag atagacgccg atacagatcc
    10201 ccaacatcac caccagcgag tagagctgcc agggtaaaaa ggtcatcaga taccagaacg
    10261 gaatcgctgc cagcgatccc atcgtgccag gaacgattgg gcttaatcca cttccgaatc
    10321 cgacagcaag cagatgccac ggattactca tcttcaggcg acttttcgcg acatctttat
    10381 ggcgtggcaa aatggtcata tcctttccag tcaaacgtga caggttcgcc gtcacgaata
    10441 aaacaaagcc cttcgatatc ggcggtcatt tgcccgatac aggtaaacgg tacgcccagg
    10501 tgtccgagag ccacatccag cgcgccacgg ttcagttccg gcacagtgaa acacaactcg
    10561 taatcttcac cgccagagag cgcccagcgc agcgcctgtt ccggttcaac atggcgagaa
    10621 agcgcatcag aaaacggcag caatgccagg tcaatacgtg cgccgcagtc gctggctttc
    10681 acgatatgcc cgagatcgga aatcagaccg tcagagagat cgatagctga atttgccaga
    10741 tcgcgcagtg cctgtccctg taaaatacgc ggcgatggac ggagatgacg tttgatcaag
    10801 tagtccgcat ctttagcatc ggcaacctgc aaacggtttt gcaaaatcgc cagcccggcg
    10861 gcgctatcgc ccggtgtacc ggtcacatag atccagtcac ccggtttcgc tccagagcgc
    10921 gttaaggctc gtcccatcgg aacaaagccg tggataccca acgtcattga taatggccca
    10981 cgcgtggtat cgccgccaat gagttgcata tcgtaataat tgagaagatc aaacaaactg
    11041 tcgctgaagg actcaagcca cgcttcgtct acgtccggta aggttaatgc cagcgtcagc
    11101 caggccggat cggcccccat cgctgccaga tcgcttaggt tcaccgccag tgctttatac
    11161 gccagatcag caggatcgat atcatggagg aagtggttgc ccgccaccag cgtatcagtg
    11221 ctgatcgcca gggtctgttt ctcggggata ttgagaagag cgcaatcgtc gccgatgccc
    11281 agttcgacat caagacgaga acttcttaca cggtcaaaat aacgggcaat cagggagaac
    11341 tcgccacatg ccatacgtta tgcctcagca ggtaaaaaag aaaaggccgg cgatcgcgga
    11401 acggtcttcc gtgaatctac cggcctggat atcacttttt gttagggcga atcacaggtg
    11461 ctgctttatc gagtacgccg ttgacgaact tatggctgtc ttctgcgccg aacgatttcg
    11521 ccagttcgat cgcttcgtta atggccactt tgtatggcac atcgctacgt ttagacagct
    11581 cgtacagcgc aatgcgcagt actgcttttt ctacctgtcc cagttcttcc agcaggcggg
    11641 acaggtatgg cttcatcagt ccgtcgaggt atgcggtgtt agtcgccacc ccggccagca
    11701 gctcacggaa gtacaggacg tcaacgtctt ttacatcctg ttcagccagg aactggtatt
    11761 caacatcagc gatgtcgttc tgggacaact gccaggagta gagcgcctgg acggcacact
    11821 cacgagcgcg gcgacgagca gcaggtttca cggatttccc cttactaatt tcaggccttg
    11881 atggctttca atacattaat catttcaagc gcggtcagtg cagcttctgc acctttgttg
    11941 ccagctttgg tgccagcacg ttcgatcgct tgttcaatgc tttcagtggt cagaacccca
    12001 aaagcaaccg gaatttcgct gtcctgggca acatgcgcca ggccgttgct tgcaccacca
    12061 gcgacatatt caaagtgggc agtgccacca cgaataaccg tacccagcgc aatcaccgcg
    12121 tcgtatttac cggttttagc cagtgcaccc gccgccagcg gcagctcata ggcaccaggc
    12181 acccaaacaa cggtaatgtt ttcatctttt acctgaccga tacgtttcag tgcgtcaatt
    12241 gcaccttcca gcaggctgtc attgataaag ttgttgaaac gcgcgatggt gatggcgacg
    12301 cgagcgtccg gggtagcaac gttagcttca ataatgttca ttttcttcct tcgggttcga
    12361 gtatggcccc gcaggggggc ggattttagc ataatatttc gtgcgctgct tccctttcga
    12421 gccgggagat catgcaccca ctaaatgcag gcaaacatcc gggcctacat gacgtatctc
    12481 tttgaattta aattgggggg cgtcggctaa tttctcaagc cctggcagcg agcataatcc
    12541 acgggcgtcg ctgcctaata gtttaggtgc gatatagaca atcagctcat cgactaaacc
    12601 cacctgcagc aatgcgccag cgagcgttgg ccccgcttcc acccagatgc tgttaatttg
    12661 ctgtttaccc agttgcatca tcagtacaac cagatccaga tgacctttat gctctggaat
    12721 cagcaaggta cgcaccgttt ccggccactc acgagaatct tcctgcgtac gcgcgaacca
    12781 ggtttcgccg ggctgctgca caatgcgatg ttccggcgtc acgcgatttt ggctatcaat
    12841 cacaatacgt accggctgac ggagattttg ttgcgggtag agcacctgag tttgttcatc
    12901 cagttcggac caacgcaccg ttaaggcagg atcatccgcc agcaccgtgg cgctgctggt
    12961 taaaatggca tgactttgcg cgcgcagtcg ttgtacgtcg cgccgcgcct gagttgaagt
    13021 gatccactgg ctttcgccgc tcgccatcgc cgtgcggcca tcaagcgatg cgccaagttt
    13081 taactgaata taaggaaagc cggtgcgcat ccgcttgaga aagcctttat tcaattgctc
    13141 ggcttcactc atcatcagcc cgtggctgac gtcaatgcca gcctgttgca gacggtaaag
    13201 tccacgccca gcgacctgcg ggttaggatc ttgcatcgct gcgaccacgc gcgctacgcc
    13261 agcggcgatg agtgcgtcac agcacggtgg cgtacgacca tgatggctac agggttcgag
    13321 tgtgacatag gcggtcgcac ctttggcttt ttcacccgcc atacgcaacg cgtgtacttc
    13381 ggcatgtggt tcacccgctc gttggtggta accttcaccg acaatttcgc catctttgac
    13441 aatgacgcac ccgacattcg ggttgggatg cgtggtaaaa cgtcctcgtt gcgccagctt
    13501 tagcgcccgc gccatgtaat actcgtcctg cacggcttag tcctccaggc gcgcgatctc
    13561 ttcgccaaat tctttgatat cttcgaaact gcggtagaca gaggcaaaac ggatataggc
    13621 gactttatcg agctttttca attgctccat caccagattg ccaatcatct tgctcggtac
    13681 ttcgcgctca ccggtggcgc gcaactgcga tttaatatga ttgattgcca tttcaacgtc
    13741 atcggaactc accggacgtt tttccagcgc ccgcagcatt ccgctgcgca atttctcttc
    13801 attaaacggt tcacgcacgt cgttgctttt tacaacacgc ggcataacca gctccgccac
    13861 ttcaaaggtg gtgaaacgtt cattacacac cagacactgc cgacggcggc gtacggatga
    13921 accctcgccc acgagacgag agtcaattac cttagtgtcc acggcgaaac agaatgggca
    13981 atgcatacgg cgtcctgacc aggtggttaa cggaaatcta ttttaccctg aactgacgat
    14041 acaacaaagg cgcaacgctt ttgagataaa ggatcgatgc tttcgctgct tttaccctct
    14101 accattaggc taattacgac gagaaaggga cgacctatga atactaacgt ttttcgactg
    14161 ctcctgctgg gaagcctgtt cagccttagt gcctgtgtgc agcaaagcga agtgcgacag
    14221 atgaaacaca gcgtcagcgc gctgaaccag gagatgacgc aactcaatca agaaacagtc
    14281 aaaatcactc agcaaaacag gctgaatgca aaatccagca gtggggttta ccttctgcct
    14341 ggagcgaaaa caccggcaag actgaaaagc cagatcggta ctttacgtat gtcgctggtg
    14401 aatattacgc ctgatacaga tggcaccacc ctgacgctac gtattcaggg tgagtcgaat
    14461 gatcccttgc ccgcattcag cggaactgtt gaatatgggc aaattcaggg aacaatagac
    14521 aactttcagg aaatcaatgt gcaaaatcaa ttgattaacg cccctgccag cgtcctcgcc
    14581 cccagcgatg ttgatattcc gttacagtta aaggggattt ctgtggatca gttaggcttt
    14641 gtacgtatcc acgacattca acctgttatg cagtaaacgt atttcggggc gattttgtgc
    14701 gtcccgcaac atctttcccc gtcattttgt tactctgctt acatcaccca gattgatagt
    14761 aaaagtttgc aacaagggtg aaagtcagta caatccctgc ccgaatgtgt gtaaacgtga
    14821 acgcaatcga ttacgtaaat gatagaactg tgaaacgaaa catatttttg tgagcaatga
    14881 tttttataat aggctcctct gtatacgaaa tatttagaaa cgcaatttgc gcctttttca
    14941 ctcccgcaag ggattttcaa acagtggcat acatatgaaa aaaacattac tggcagccgg
    15001 tgcggtactg gcgctctctt cgtcttttac tgtcaacgca gctgaaaacg acaaaccgca
    15061 gtatctttcc gactggtggc accagagcgt taacgttgtc ggaagctatc acacccgttt
    15121 cggaccgcag atccgcaatg atacctacct tgagtacgaa gcattcgcta aaaaagactg
    15181 gttcgacttc tatggttatg cggatgcgcc ggtattcttc ggcggtaact ccgatgcaaa
    15241 aggtatctgg aaccacggtt ctccgctgtt tatggaaatc gaaccacgtt tctccatcga
    15301 caagctgacc aatactgacc ttagcttcgg tccgttcaaa gagtggtact tcgcgaacaa
    15361 ctacatttac gacatgggtc gtaataaaga tggtcgccag agcacctggt acatgggtct
    15421 gggtaccgac atcgatactg gtctgccgat gagcctgtcc atgaacgtct atgcgaaata
    15481 ccagtggcag aactatggcg cagcgaacga aaacgagtgg gacggttacc gtttcaaaat
    15541 taaatacttt gtgccgatta ccgatctgtg gggcggtcag ctgagctaca tcggcttcac
    15601 caacttcgac tggggttccg atttggggga tgacagcggt aacgcaatca acggtattaa
    15661 gacccgtact aataactcta tcgcttccag ccatattctg gctctgaact acgatcactg
    15721 gcactactct gtcgtagctc gttactggca cgacggtggt cagtggaacg acgatgcaga
    15781 actgaacttc ggcaacggca acttcaacgt tcgctctacc ggctggggtg gttacctggt
    15841 tgtaggttac aacttctgat tatgaaaatg ccgggattta ttcccggcat ttcagattgt
    15901 taacaacgtg cgctttgttc atgccggatg cggcgtaaac gctttgtccg acccacaaaa
    15961 gcacgtaaat tcaatatatt gctgaaataa cgtagtcctg ataagcgtag cgcatcaggc
    16021 aattttacgt tttaatcact tcttcttgtt catcatcgct ttcaggtcag cgaatgggtt
    16081 gtacttcgct tcaccgacat ctttctgcgc atcttcccct gcgataacgg tcgtaccata
    16141 ctgatccgct tcggtatatt tcgaatgctc atgatcgtgg caatagagac aaagcaattc
    16201 ccagttactg ccatcttccg ggttattggt atggtcgtga tcaatgtggt gaacagtaag
    16261 ttcacgcagg ttggaataga caaactcgcg ggaacagcga ccgcagaccc acggatagat
    16321 ttttaatgct ttttcgcgat agccgctttc taaccgcgca tagttttttg ggatgatagc
    16381 catgaaaagt tacctggatg aaatgtaggg ttacgcgcag tttcccaaga tgcggggatt
    16441 ttcgcaatct ccatacatca aaaaaaatcc cgatcttctg accgggattt tcaacatcaa
    16501 cgggaacttg attaaggcag aattgacggc tgatccgccc cttctttttc cactttctgc
    16561 tgcaacatgt gttcgcgctt catacccagt ttcagagcca acgcagatgc cacatagatg
    16621 gaagatgcag taccgatgga aacaccgata agcatggtca gcgagaagcc ttccagtacc
    16681 ggaccaccga agaggtacag catcaggata accatcaagg tagtaccgga tgtgatcaag
    16741 gtacggtgca gcgtctgggt caaggacacg ttaaagattt cgtaaggcgt accgcgacgg
    16801 atcttgcgga agttttcacg aatacggtcc gataccacga tactgtcgtt aagcgagtaa
    16861 ccgataaccg acatcaacga tgccacaatg gtcaggtcaa tctcgatatg gaataacgac
    16921 aaaataccca gcgtaatgat aacgtcgtgc gccagcgcaa taaccacccc tgccgccagt
    16981 cgccactcaa agcggaaacc tacgtacacg aggatagaca gcagcgctgc catcaacgcc
    17041 atcgcaccgg tttgcgcaag gtctgccccc acgctcggac cgacgaactc aatacgcttc
    17101 actgctgcat tctgattggt ggattcgtta atcaccttca gaacctggct gcccagcacc
    17161 tgaccgccgg tttcgccttc agcaggcggc atacggacca tgatgtcgtg gctgctacca
    17221 aagttttgca gcattggctc ttcaaaaccg gctttttgca atgcatcacg cattacgtca
    17281 atttcagccg gtttttcgag cgtaatttcg ataaccgtac caccggtaaa atccagcccc
    17341 cagttaaagc cgcgcacgcc cataataacg atagcagcga ttaacagcag accagagatg
    17401 ccgaaagccc agtagtccca gcgcataaag tcatagactt tacggccgtg gtttagttgt
    17461 tcaacagtat attcctgtgc cacatcgcac tcctcagatt gacagcttct tgacgcgctt
    17521 gccgccatat agcaggttta cgatggcacg cgtaccgaca atcgcggtaa acatcgacgt
    17581 cgccacaccg ataccggtag taatcgcgaa ccctttaatt gccccggtac ccactgcgta
    17641 caggatgatg actttaatca gcgtggtgat gttcgcatcg aagatagaac tgaatgcgcc
    17701 acgataacct tcatcaattg cctgttgaac agtacgtccg ttgctcaact cttctttaat
    17761 acgttcgttg atcagtacgt tcgcatcgac cgccaccgca agggttaaga cgatacccgc
    17821 gatacctggc atactcagcg ttgcgcctgg caacagcgac ataatgccga cgattaagat
    17881 caagttggca atcagagcac tggtcgcaat cagaccaaac ttcttataga agatgatcat
    17941 gaacagaata gaaaccagca gaccggcaag gcaagcctcc agcccctgtt caatgttctg
    18001 catacccagg gttgggccaa tggtacgttc ttcaacaatc tgaatcggcg cgatcaacgc
    18061 accggcacgc agcagcagtg acagctgacg ggcttcgttc gggttgttga tgccggtgat
    18121 acggaagctg ttacccagac gagactggat gttggcgatg ttaatcacct cttcctgttt
    18181 caccagaacc gcacgaccat ttgcatcttt cttaccgctg tctttgtact ccacaaacag
    18241 ggttgccatc ggtttgccga tgttgtcctt agtgaagtta gacatgatgt taccaccagc
    18301 gctatcgagc gagatgttaa cctgcggttg gttgtattcg tcctggctgg aagtggagtc
    18361 ggtgatatgg tcaccggtca gaattacgcg tttgtacagc acaactggct gaccttcgcg
    18421 ggtctgtttc acttcagagt cgcccggtac gcgaccggat gccgcagcgg cctggtcaac
    18481 gttggtgttt accagacgga attccagcgt tgccgtcgca cccagaatct ctttcgcacg
    18541 cgcagtgtcc tgaatacctg gcagttcaac aacgatacgg tcagcaccct gacgctgaac
    18601 caccggctcc gccacgccaa gttggtttac acggttacgc aggatattaa tgttctgctg
    18661 caccgcatat tcacgcgctt cactcagacg agcatcgctc attaccgcac gcagctggtt
    18721 gctgccctgg ctgctaatca ccaggtccgg atggcgcttg ctcagatacg caatggcttc
    18781 atcacgagct ttagcatcgc ggaaagtaat gctcaggccg tagttgtttt ctttacgaac
    18841 ggtggtatac gggatgccct tttcacgcag atcgctgcgc aggctatcga tattttgttc
    18901 ctggagtttg ccaagcgcgg tgtccatatc cacttccatc aggaagtgaa cgccgccacg
    18961 caggtcaagg ccgagcttca tcggctcagc gtgaatagct gccagccagc gcggcgttgc
    19021 cggggcaagg ttaagcgcca cgacgtattt gtcacccata acgcccatta atgcttcacg
    19081 agcgcgcagc tgggtgtcag tggagtcgaa gcgcgcaaga atagcgccct cttccagtgc
    19141 cacagactta gcagttattt tttcttcttg taacgttttc tggacctgga tcagcgtttg
    19201 ctcactggcg gcgactccgc gcgcaccagt gatctgaaca gccggatcct caccaaacag
    19261 gttgggaagc gcatacagca gaccgatgac aatcaccacg atcagcatga cgtacttcca
    19321 caaaggataa cggtttaaca cggcaattcc cttagggaaa aattttaatt acagcgcctt
    19381 catggtgcct ttcggcagga cggcagctac gaagtcacgt ttaataacta cttcagtggt
    19441 gtcattcagc gcgatagcaa tgtagccgtt ttccgctact ttggttacgc ggccaaccag
    19501 gccaccgttc gtcagaactt catcaccttt ggcaatggag tccatcagct ttttgtgttc
    19561 tttggtgcgc ttctgctgtg gacgcaggat catgaaatag aaaatcagac cgaataccac
    19621 cagcatcaaa atcaaagaca tcgggctacc ttgcgccggt gcacccgttg ccgctaccgc
    19681 atcagaaata aaaaagctca ttaaatttcc ctcattatta atattaatca acgttcaaag
    19741 gtggtacttc tcgcccctga cgctggtaaa aatcagttac gaagctctct aatttaccct
    19801 cttcaatagc cttgcgtaaa cccgccatca aacgctggta gtaacgaagg ttatgaatgg
    19861 tgttgagtcg cgcgcctaat atttcgttgc aacggtcaag atgatgcaag taagcgcgtg
    19921 aataattgcg acaggtgtag caatcacact caggatcgag tgggccagta tcgctcttat
    19981 acttcgcatt gcggattttc accacgccat cggtcacgaa caaatgacca tttcgggcgt
    20041 tgcgggttgg cattacgcag tcaaacatat cgataccacg acgtacgcct tcaaccaggt
    20101 cttctggttt accaacgccc atcaggtaac gcggtttgtc tgccggaatt tgcgggcata
    20161 catgctccag aatgcggtgc atatctgctt tcggctcacc cacagccaga ccgccgacag
    20221 cgtagccatc aaaaccgata tctaccagac ctttaacaga aatatcacgt aaatcttcgt
    20281 aaacgctgcc ctgaatgata ccaaacagcg catttttgtt tccgagactg tcaaaacgct
    20341 cacggctacg cttcgcccaa cgcagagaca tctccatgga gcgttttgcg taatcccagt
    20401 cagcaggata cggcgtacac tcatcaaaga tcatgacgat atccgaacca agatcgtact
    20461 gaatctccat tgatttttca ggatcgagga aaatcggatc gccgttgatc gggttacgga
    20521 agtgcacgcc ctgttcggtg attttacgaa tatcgccaag gctgaagacc tggaaaccgc
    20581 cggagtcggt gaggatcggc cccttccact gcataaaatc gtgcagatcg ccgtgcagtt
    20641 tcatgatttc ctggcccggg cgcagccaca ggtggaaggt gttgccgagg ataatttgcg
    20701 cgccagtggc ttcaacttct tccggcgtca tcccttttac ggtgccgtag gtgccaacag
    20761 gcataaaaca aggcgtttcc actacgccac gatcaaagac caggcggcca cggcgtgcgc
    20821 gaccgtcggt ggtgtccagt tcaaatttca ttttttctcc actacgtcag aaaaacagtc
    20881 caacgtttta aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga
    20941 ttgtacgtga taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgct
    21001 gctttatagg cgttcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc
    21061 gtcgactctg gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc
    21121 ggatagataa agatttgggt atcgtcgaag aacggttcaa tgagatcgtt tttcgctgcc
    21181 tgagccgcgc tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg
    21241 cgcgctttcg ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag
    21301 tgcatgatgt gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg
    21361 tgcaacgtca caaacgccat ctccacgcct ttggcgcgca atttttccag caaaggctcg
    21421 tcaaaatgca gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt
    21481 tgataaagtt cgcggtcagc gtcttcgtcc ggacggtcga tatacggcgg cagcggcata
    21541 tggccgatac tgttgaggat atccagtacc gaacgatcat cattgaattc gacttcaaac
    21601 agtgcgccgt ggcgcgcggt cattgttgcg ttaatacttt cgtcatcgcc cagcagcagt
    21661 tctgcgccag gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg
    21721 agcatccgct caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacaggcgc
    21781 gccgggatca cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcaagt
    21841 aaatcggtga aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga
    21901 cagctactgc gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag
    21961 aaatcggtaa cgcgcatgac actgactcag actaaaataa gaggcgggta gtctagtgcc
    22021 ggggcgctct ccctgcaacc tttaccccct tttggataga atataatcga tgaatttttt
    22081 agctcacctg catttagccc atctcgcgga aagctcgctt tccggcaatt tactggctga
    22141 tttcgtacgc ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat
    22201 gcatcgacgt atcgacgtat taaccgacaa tctgccggaa gtccgcgaag cacgggaatg
    22261 gtttcgtagt gaaactcgcc gcgttgcgcc tattacgctg gatgtcatgt gggatcactt
    22321 tctttcccgc cactggtcgc agctgtcgcc ggactttccg ctacaggaat ttgtctgtta
    22381 tgcccgcgag caagtgatga cgattttgcc ggactcaccg ccacgtttta tcaatctgaa
    22441 caattacttg tggtcagagc agtggctggt gcgctatcgc gatatggatt tcatccagaa
    22501 cgtgttaaac ggcatggcaa gccgccgccc acgtctggat gccctgcgtg actcctggta
    22561 cgatttagac gctcattatg acgccctcga aacccgcttc tggcagtttt atccgcggat
    22621 gatggcgcag gcgtcacaca aggcgttata aaacgttagt tcatccatac cgtagccgaa
    22681 atggcaggca aagccagaat cccgtttgtc agttgcccat ggccttcttt gcgttgccat
    22741 tgcgcgacat taagaaacgg tgacgcgggg agcaccactt cacaggcctc gccacggttg
    22801 attgccacca gtacacgttg ctgattcagc acgcggacaa ataccactac gttatcttcc
    22861 gcatacagca cctgacagcc gccacgacgc agcgcctgac ttttcttacg cagcgcaatc
    22921 attcgctggt acagcgcgaa taacgccgta tcctgctttt ccacctgcca ggggaacggt
    22981 ttacggcaaa acggatcgtt tttgccatcc agtcctactt catcaccgta ataaatgcac
    23041 ggtacaccag gccaggtgaa cagccagacc accgccagcg gcaggcgcgc aatatcccga
    23101 ccgagcagcg ttttaaatcg cgcagtatcg tggctgtcga gctgattaaa catacgtaat
    23161 tgttgttgat gagaaagccc tgcgcggtaa ttatccatcc aggccataca ggtttgggca
    23221 tcaatttgct gcggatcgta agagatatcg gtattggcaa gaaatcccca caacgggaat
    23281 gtgaagccac gatagttcat ggcggcatct tccacatcgg cctgtaacca ttgccgtgca
    23341 tcgccaaaat gttcgccgac aatatacgct tccggctggg tttctttcgc cgcttcggtg
    23401 atcccagcaa cgtgctgcat attattgcgc gccccacccg cctcccccag catatgcacc
    23461 acatccagcc gccagccgtc catactccac ggcgctttca gccaatggcg gacaatactg
    23521 tcttccccgc gataaatttc attcaccaga ctttccgact gataatccag cttcggcaag
    23581 ctggcatagc caagccagtc gagcgccgtg ccatcatcac taaacgagta ccagtcgcgc
    23641 cagggcgatt cagggttgtg acaagctccc cccgtgccac gattgtgcct gtcaaaccag
    23701 gcatgggaat cgccactgtg gttaaacacg ccatccagca ccagccgcat tcccagctgc
    23761 tgcgtattgt ggcgtaagcg cagcaacgcc ccatcaccgc caaactgcgg atcgacatgg
    23821 cgataatcct cggtatcgta tttatgtacg ctgggagctt taaacaccgg attgagatac
    23881 agcgccgtca cgccaagctt tttcagatac ggcagttttt cgcttatccc gtccagatcg
    23941 ccgccataga acgttgatcc gcccgcctgc gccgtgaccg gttcatccca gtcacgcaag
    24001 atgatctctt gtccggctgc atgatggtaa tagacatgat cctgttcagc ttcacgagga
    24061 agactacgcg caaaacgatc agggaagatc tgataaaaaa tctgatccgc agcccattgt
    24121 gggccgatat ccggtacatc gacggcaaac tgctccagtc gtgccggcgg cattcggctg
    24181 aagccctgcg gtgtaaacca acgctggcga tcgtgccaca gcagtttgaa actgtaacgc
    24241 cgccggggtt gtccgctgga gagatcaatc gccgcacgcc atgcggtgac gccaggctgc
    24301 tgctgactgc gctgcttatg catcgatact gacatttctt cgttatcgtg ttctgtacgc
    24361 agcataatgc gctgcggtgg gtcttcgccc gtcagccaca gtgtaatgag cagttgatct
    24421 ttgctttgtt taacaaatgg gggcaccggc aggtgccatg catttaacat cataattccc
    24481 ctgtgatgaa aatctgatca ccttgccata gcctaatgca ggatcactca tcatctgaag
    24541 gatatttggg ggaggaggac gggggcggat aactttcgga atagccggat caggatttcc
    24601 tcctgaaccg gcttacagaa cgcaaggatt actggttttc agccagctga cgatcgtggc
    24661 ggcgtttaaa catccagcca atcaacagca caacaatcca cgcgaaaccg acatacagcg
    24721 agatacgcgt atccgggtga taaccaatca acccgataat aaagagcagg aaaatcaaac
    24781 cgccgatggt cgttgctacc ccacccggca ctttaaattt cagcgcctta acttcttctg
    24841 gcggcaaacg gcgacggaag gcaatttgcg acagcaggat cataatccac acccacaccg
    24901 tggcgaaggt tgccagcgaa gcgatcacca ggaagacgtt ttccggcatg atgtagttca
    24961 gatacaccgc aaacagcagc gcggtagtca tcaccagcac cgtaacccac ggaataccgc
    25021 gacgcgacgt tttgctgaaa attttcggcg cgctgccctg ctctgccata ccgtggagca
    25081 tacggcctac gccaaatacg tcactgttaa ttgccgacag cgaagcagtc agcacaacaa
    25141 agttaagaat gctggcggca aaggtaatgc ccatatgctg gaacgtcagc acgaacgggc
    25201 taccggcagt gccaacctga ttccacgggt agatagacat aatgacgaac agcgtaccga
    25261 cgtagaacac cagaatacgc atcggcacgg agttaatcgc acgcggtatc gatttctcag
    25321 gatctttcgc ttcaccggcg gtaatcccga taatttcgat cccaccgtaa gcaaacatca
    25381 ccatttgcaa cgacatcacc atcccaagcc agccgttact gaagaagccg ccgttgctcc
    25441 acaggttatg aataccggtc ggttgcccgc cgttgccaat cccccagatg atgatgccga
    25501 aaccggcgac aatcatgatg atgatggtgg cgactttaaa gaacgagaac cagaattcca
    25561 gctcaccgaa gaccttcacg ctcatcaggt ttacggcgca aatgatcagc accacgctca
    25621 gtacccaaat ccagtgcggc accgtcggga accagacacc catatagata ccaaaagcgg
    25681 tcacatcggc gatggcgaca ataaggattt caaagcaata ggtccagccg gtaatgtaac
    25741 ctgccagcgg gccgaggttt tcctgcgcat aacgcgagaa agagctggct gccgggttat
    25801 gtaccgacat ttcccccagc gcacgcataa tgatatacgc cgcgatacca ccgataatat
    25861 aggccaacaa cacgctcgga ccggccattt tgatggcgtc tgccgaaccg taaaacagcc
    25921 cggtgccaat tgctgaaccc agtgccataa agcgtatgtg gcgggtactt agcccacgct
    25981 ttagcttgtt cttactttcc atcaattcct aacccatcaa cacaataaaa aaccacgggg
    26041 catcgcagcc ccgtggttaa aacaaatgtt cagtgattta gtgagcgctg gaggtcacct
    26101 gacgacctgc cgcgcgatcc cagataatgg ccagaaccac catcaccact gttggcatta
    26161 accacgccag accttgttcg gccagcggta aacgctgcgc ccaggacggt aagatatcgc
    26221 tgaatgcaga tgctttgatc ccgtcgagaa taccaaaaag caggctgata aacatcggcg
    26281 gagcaatcac gcgggacgaa ttatgccacc atgagcgtgt aaaacttaat acaaccagtg
    26341 cgatacacgg cggataaata gcggtcagca ccggtacgga gatctggatc agctggctta
    26401 agccgaggtt agaaaccacc atcgagaagc cgccgaggat aaacaccagc gtacgataag
    26461 agagcggtac gtactgggca aagaattctg cacaagcaca ggtcaggcca actgccgtta
    26521 ccaggcaggc gatgaagatt aacgccgcca gcaggaagct accgccgccg ccaaaggtgt
    26581 gctgaacgta agcatgcaga atagcagcgc cgtttgcaga ctgatcgacc agcgacgcgc
    26641 tgtctgaacc cagacggaac agcgccaggt acagcagagt cagaccaaca cccgccatca
    26701 ggccagccca gacggtataa cgggtcagca gacgcgcttc ggtaacgcca cgagaacgcg
    26761 ccgcgttaac aataacgata ccaaacacca ttgcgcccag cgtatccatg gtcagatagc
    26821 cgttaacgaa gccgttagaa aacgcagcgt tttgataagc ctcagtcgcc gtgctgatag
    26881 aacccgccgg ccagacaatc gcggcaacag acaggatgac cagcgcgata attttcagcg
    26941 gcgcaaggaa gttgcccacg gtatccagca gcttgcccgg atagagcgaa accagaataa
    27001 cgatagcgaa atagaccagg ctgtagataa acagcggcag cgcggaatca ccagtcagcg
    27061 gcgcaatccc cacttcaaag gaaacggtag ctgtacgcgg cgtagcgaaa agcggcccca
    27121 ccgccaggta acaaaccgtt gccagcagta cgccagcgac tttaccgatt ggcgtgctga
    27181 ggctgtcaac accgccgcca acttttgcca gcgccactac cgttagtacc ggcagaccaa
    27241 cggcagtaat gaggaagccg aatgccgcag tccagacgtg ttcgcctgcc tgcaagccga
    27301 ccattggagg gaaaataatg ttacctgcgc cgacgaacaa cgcaaatgtc ataaagccca
    27361 gagcgatgat atcgcgcgat cttaattgat gggtcataaa tcatactgcc tgtggatgtg
    27421 gtgttgtaac gttaaatttt tgtgccaatc cctggggatg atacagcggt atatttaaac
    27481 aattttagcg ggaaatactc ccaaccaggc gcggcgaaga atagtttttc gatttaccgc
    27541 gttaatgcag tcagtatgac agcacctgaa gcgcaattta aacgcttata acgttttaaa
    27601 gcaaggaggg atctaaaaac cagaggtata tagcgataag aaatcaccaa ccagtataaa
    27661 acactatttc cgtgaaaaag acatggctaa tcatgcgaac aaaaaatcac caaccgttgt
    27721 aaaatctaac gcccgtattt tgactgcaaa gaaaataagc cagcatcggc tggcttatgg
    27781 caataaaaga tgacaaaggc ggattaatcg ctgtttttgg caattaaacg ttccgggata
    27841 acaaaactga aacgtgttcc ttttcctact gtactctcaa tattcaggcg actttcgtga
    27901 tgattcaccg catgtttcac gatcgctaac cctaatccgc taccgccggt ttgccgggaa
    27961 cgcgctttat caacgcgata aaaacgctcg gtcaggcgcg gaatatgctc cggtgcaatg
    28021 cccggtccgt tatcttcaac gctaaattcg gcaccgtgcg gcactcgctg ccagcgtacg
    28081 gtgatatgcg tgccttctgg agtatgattc acggcgttat agaccaggtt cgaaatcgca
    28141 ctgcgtagct gatcttcgtt gccagacact ttgaggccgt tatctatctc aaaggtaaat
    28201 gtctgttttt tctgactcag agtctgagcc tcacgctcaa caacgcgcag catcatcggc
    28261 acatcaacct tttcattgag caaatgcgtc ggtgcggctt ctattttcga cagcgtcagc
    28321 aactgcttca ccagcccttc catccgctgg gtctgctcgc gcatggtgtg caacgctttt
    28381 tcgcgtaccg cgccttccag cggctgctca tccatcatct ccaggtaacc ctgtaacacg
    28441 gtcaatggcg tacgtaactc atggctcacg ttggcaaaaa agttacgccg cgccccttcc
    28501 agttgatgca tttgcgtgac atcacgcgcc accatcagca actgtttgtg ggtataaggc
    28561 atgacgcgaa tttccagatg ccgcccggtg ttgagcacca gattgagcgg gcgagaaaaa
    28621 tcacgcgttt tcagatattg cgtaaactcc gggtaacgca gcaggttaag gatgttctgc
    28681 ccgttatctt ccggccagcg caaaccaaga atttgttgcg ccagaccgtt acaccagaaa
    28741 ataccgccct cttcagtggt cagcaccact gcgtcgggca gcgactccgc gccgctacga
    28801 aagcgtttaa tcagattgcc cagttcacgg cggcgttttt tatttcgcag ctgcatctgg
    28861 tgtaagccgt atagtagcgg ttcccagcta ccacgccccg gtggcggggt catactacga
    28921 tccacccaca gccaccatga aaggcgcaat aaattccaga aatgccagat aagcagtccc
    28981 gttaccgatg ccagcaaaaa ccagggcagg taaccaaaaa atgcacccag gatgaaagcc
    29041 gggaagcagc aaagtagcag ctccagcacc agccttttcc acgacagccg ttccagcacg
    29101 taagatactc cagttaagaa atcataagcc ctgctctgcg tccgatgagc aaggcgttaa
    29161 aagcgggttg aaaaacgata tcctgtaccg cgcacggtct gcaccatgcg gtcatgcccg
    29221 cctggctcca gtgctttacg taggcgacga atgtggacat cgaccgtacg atcttccaca
    29281 tacacgttag ttccccagac gtggtttaac agctgctcgc ggctgtacac gcgctcagga
    29341 tgcgtcataa aaaagtgcag cagtttaaat tctgtcggcc ccatctccag cggctcttcg
    29401 cccgccatca ctcggtgaga tgtcgggtcg agacttaatc cctgcatctc aatcacctct
    29461 tccaccgcca ttggcgaaat acggcgcatt accgctttga ttcgcgccac cagctccttc
    29521 ggcgaaaacg gcttggtgat atagtcatcc gcgccggttt caaggccgcg cacgcgatct
    29581 tcttcttccc ctctggcggt caacatcacc actggaatat cccgggtcat cgactcgcgc
    29641 ttgaggtgtt tgatgaactg gataccggag ccgccaggta acatccagtc aaggagaatt
    29701 aaatccggcc agggttcatt cagttgattc acagcactgt cataatcttc cgcttcgacc
    29761 ggctgaaagc cattttgttc gagcacgaag cagaccattt cgcgaattgg agcttcatct
    29821 tctacgacca gaatacgtct cgccatgatt tgccctgttg taataaatag gttgcgatca
    29881 ttaatgcgac gtcattatgc gtcagattta tgacagattt atgaaaagct cgtcgcacat
    29941 atcttcaggt tattgatttc cgtggcgcag aaaaaagcaa atggcacatc tgtttgggta
    30001 taatcgcgcc catgcttttt cgccagggaa tcgttatgcg catccttcac acctcagact
    30061 ggcatctcgg ccagaacttc tacagtaaaa gccgcgaagc tgaacatcag gcttttcttg
    30121 actggctgct ggaaacagca caaacccatc aggtggatgc gattattgtt gctggtgatg
    30181 ttttcgatac cggctcgccg cccagttacg cccgcacgtt atacaaccgt tttgttgtca
    30241 atttacagca aactggctgt catctggtgg tactggcagg aaaccatgac tcggtcgcca
    30301 cgctgaatga atcgcgcgat atcatggcgt tcctcaatac gaccgtggtc gccagcgccg
    30361 gacatgcgcc gcaaatcttg cctcgtcgcg acgggacgcc aggcgcagtg ctgtgcccca
    30421 ttccgttttt acgtccgcgt gacattatta ccagccaggc ggggcttaac ggtattgaaa
    30481 aacagcagca tttactggca gcgattaccg attattacca acaacactat gccgatgcct
    30541 gcaaactgcg cggcgatcag cctctgccca tcatcgccac gggacactta acgaccgtgg
    30601 gtgccagtaa aagtgacgcc gtgcgtgaca tttatattgg cacgctggac gcgtttccag
    30661 cacaaaactt tccaccagcc gactacatcg cgctcgggca tattcaccgc gcacagatta
    30721 ttggcggcat ggaacatgtt cgctattgcg gctctcccat tccactgagt tttgatgaat
    30781 gcggaaagag taaatatgtc catctggtga cattttcaaa cggcaaatta gagagcgtgg
    30841 aaaacctgaa cgtaccggta acgcaaccca tggcggtgct gaaaggcgat ctggcgtcga
    30901 ttaccgcaca gctggaacag tggcgcgatg tatcacagga gccgcctgtc tggctggata
    30961 tcgaaatcac tgctgatgag tatctgcatg atattcagcg caaaatccag gcattaaccg
    31021 aatcattgcc tgttgaagta ttgctggtac gccggagtcg tgaacagcgc gagcgtgtgt
    31081 tagccagcca acagcgtgaa accctcagcg aactcagcgt cgaagaggtg ttcaatcgcc
    31141 gtctggcact ggaagaactg gatgaatcgc agcagcaacg tctgcagcat cttttcacca
    31201 cgacgttgca taccctcgcc ggagaacacg aagcatgaaa attctcagcc tgcgcctgaa
    31261 aaacctgaac tcattaaaag gcgaatggaa gattgatttc acccgcgagc cgttcgccag
    31321 caacgggctg tttgccatta ccggcccaac cggtgcgggg aaaaccaccc tgctggacgc
    31381 catttgtctg gcgctgtatc acgaaactcc gcgtctctct aacgtttcac aatcgcaaaa
    31441 tgatctcatg acccgcgata ccgctgaatg tctggcggag gtggagtttg aagtgaaagg
    31501 tgaagcgtac cgtgcattct ggagccagaa tcgggcgcgt aaccaacccg acggtaattt
    31561 gcaggtgcca cgcgttgagc tggcgcgctg cgccgacggc aaaattctcg ccgacaaagt
    31621 gaaagataag ctagaactga cagcaacgtt aaccgggctg gattacgggc gcttcacccg
    31681 ttcgatgctg ctttcgcagg ggcaatttgc tgccttcctg aatgccaaac ccaaagaacg
    31741 cgcggaattg ctcgaggagt taaccggcac tgaaatctac gggcaaatct cggcgatggt
    31801 ttttgagcag cacaaatcgg cccgcactga gctggagaag ctgcaagcgc aggccagcgg
    31861 cgtcgcgttg ctcacgccgg aacaagtgca atcgctgaca gcgagtttgc aggtacttac
    31921 tgacgaagaa aaacagttac ttaccgcgca gcagcaagaa caacaatcac taaactggtt
    31981 aacgcgtctg gacgaattgc agcaagaagc cagccgccgt cagcaggcct tgcaacaggc
    32041 gttagccgaa gaagaaaaag cgcaacctca actggcggcg ctcagtctgg cacaaccggc
    32101 acgaaatctt cgtccgcact gggaacgcat cgcagaacac agcgcggcgc tggcgcatac
    32161 tcgccagcag attgaagaag taaatactcg cttacagagc acaatggcgc ttcgcgcgag
    32221 cattcgccac cacgcggcga agcagtcagc agaattacag cagcagcaac aaagcctgaa
    32281 tacctggtta caggaacacg accgcttccg tcagtggaac aacgaactgg cgggttggcg
    32341 tgcgcagttc tcccaacaaa ccagcgatcg cgagcatctg cggcaatggc agcaacagtt
    32401 aacccatgct gagcaaaaac ttaatgcgct tgcggcgatc acgttgacgt taaccgccga
    32461 tgaagttgct accgccctgg cgcaacatgc tgagcaacgc ccactgcgtc agcacctggt
    32521 cgcgctacat ggacagattg ttccccaaca aaaacgtctg gcgcagttac aggtcgctat
    32581 ccagaatgtc acgcaagaac agacgcaacg taacgccgca cttaacgaaa tgcgccagcg
    32641 ttataaagaa aagacgcagc aacttgccga tgtgaaaacc atttgcgagc aggaagcgcg
    32701 catcaaaacg ctggaagctc aacgtgcaca gttacaggcg ggtcagcctt gtccactttg
    32761 tggttccacc agccacccgg cggtcgaggc gtatcaggcg ctggagcctg gtgttaatca
    32821 gtctcgatta ctggcgctgg aaaacgaagt taaaaagctc ggcgaagaag gtgcggcgct
    32881 gcgcgggcaa ctggacgcct taacaaagca gcttcagcgc gatgaaaacg aagcgcaaag
    32941 cctccgacaa gatgagcaag cacttactca acaatggcaa gccgtcacgg ccagcctcaa
    33001 tatcaccttg cagccacagg acgatattca accgtggctg gatgcacaag atgagcacga
    33061 acgccagctg cggttactca gccaacgaca tgaattacaa gggcagattg ccgcgcataa
    33121 tcagcaaatt atccagtatc aacagcaaat tgaacaacgc cagcagcagc tcttaacggc
    33181 attggcgggt tatgcactga cattgccaca ggaagatgaa gaagagagct ggttggcgac
    33241 acgtcagcaa gaagcgcaga gctggcagca acgccagaac gaattaaccg cgctgcaaaa
    33301 ccgtattcag cagctgacgc cgattctgga aacgttgccg caaagtgatg atctcccgca
    33361 cagcgaagaa actgtggcgc tggataactg gcggcaggtt catgaacaat gtctcgcatt
    33421 acacagccag cagcagacgt tacagcaaca ggatgttctg gcggcgcaaa gtctgcaaaa
    33481 agcccaggcg cagtttgaca ccgcgctaca ggccagcgtc tttgacgatc agcaggcgtt
    33541 ccttgcggcg ctaatggatg aacaaacact aacgcagctg gaacagctca agcagaatct
    33601 ggaaaaccag cgccgtcagg cgcaaacgct ggtcactcag acagcagaaa cgctggcaca
    33661 gcatcaacaa caccgacctg acgggttggc tctcactgtg acggtggagc agattcagca
    33721 agagttagcg caaactcacc aaaagttgcg tgaaaacacc acgagtcaag gcgagattcg
    33781 ccagcagctg aagcaggatg cagataaccg tcagcaacaa caaaccttaa tgcagcaaat
    33841 tgctcaaatg acgcagcagg ttgaggactg gggatatctg aattcgctaa taggttccaa
    33901 agagggcgat aaattccgca agtttgccca ggggctgacg ctggataatt tagtccatct
    33961 cgctaatcag caacttaccc ggctgcacgg gcgctatctg ttacagcgca aagccagcga
    34021 ggcgctggaa gtcgaggttg ttgatacctg gcaggcagat gcggtacgcg atacccgtac
    34081 cctttccggc ggcgaaagtt tcctcgttag tctggcgctg gcgctggcgc tttcggatct
    34141 ggtcagccat aaaacacgta ttgactcgct gttccttgat gaaggttttg gcacgctgga
    34201 tagcgaaacg ctggataccg cccttgatgc gctggatgcc ctgaacgcca gtggcaaaac
    34261 catcggtgtg attagccacg tagaagcgat gaaagagcgt attccggtgc agatcaaagt
    34321 gaaaaagatc aacggcctgg gctacagcaa actggaaagt acgtttgcag tgaaataact
    34381 attcagcagg ataatgaata cagaggggcg aattatctct tggccttgct ggtcgttatc
    34441 ctgcaagcta tcactttatt ggctacggtg attggtagcc gttctggtgg ttgtgatggt
    34501 ggtatgaaaa aagtcatttt atctttggct ctgggcacgt ttggtttggg gatggccgaa
    34561 tttggcatta tgggcgtgct cacggagctg gcgcataacg taggaatttc gattcctgct
    34621 gccgggcata tgatctcgta ttatgcactg ggggtggtgg tcggtgcgcc aatcatcgca
    34681 ctcttttcca gccgctactc actcaaacat atcttgttgt ttctggtggc gttgtgcgtc
    34741 attggcaacg ccatgttcac gctctcttcg tcttacctga tgcttgccat tggtcgcctg
    34801 gtttccggct ttccgcatgg cgcatttttt ggcgtcggcg cgatcgtgtt atcaaaaatt
    34861 atcaaacccg gaaaagtcac cgccgccgtg gcggggatgg tttccgggat gacagtcgcc
    34921 aatttgctgg gcattccgct gggaacgtat ttaagtcagg aatttagctg gcgttacacc
    34981 tttttattga tcgctgtttt taatattgcg gtgatggcat cggtctattt ttgggtgccg
    35041 gatattcgcg acgaggcgaa aggaaagctg cgcgaacaat ttcacttttt acgcagcccg
    35101 gccccgtggt taattttcgc cgccacgatg tttggcaacg caggtgtgtt tgcctggttc
    35161 agctacgtaa agccatacat gatgtttatt tccggttttt cggaaacggc gatgaccttt
    35221 attatgatgt tagttgggct agggatggtg ctggggaatg tgctaagtgg caggatttca
    35281 ggacgttatt caccactgcg cattgcagca gtaactgact ttatcattgt actggcactg
    35341 ctgatgcttt ttttctgcgg cggcatgaaa acaacgtcgc ttatttttgc ttttatttgt
    35401 tgcgcgggat tatttgccct ttcagcacct ctgcaaatat tgttactaca aaacgccaaa
    35461 ggcggagagt tattaggtgc cgcaggtggg caaatagcgt ttaacctcgg tagcgccgtc
    35521 ggcgcatatt gcggaggtat gatgctgacg ctggggctgg catataatta cgtggcgctg
    35581 cctgccgccc tgctttcgtt tgctgcgatg tcatcgttgc tgctgtatgg tcgctataag
    35641 cgccagcaag cggcggatag tccggtgctg gcgaaaccac tggggtagat tactgtcacg
    35701 gtgcgcgttg cttattgccg gatgcggcgt gaacgcctta tccgccctac gcggttccgg
    35761 cacattttgt agatactgat aagacgcggc aagcgtcgca tcaagcatga tgcgccaatt
    35821 gcctacgttt tttactcttg tggccataac cacgcagcgc cgcgtacgcc gctggaatca
    35881 ccgtgcttcg ccttacgcac cggcgtttca cattcgccgc cgaagacaaa ttgtttaatc
    35941 aactgcccaa ccgtttgata taaacggtct acattgctca tcccgccccc caggacaatc
    36001 acatccggat cgagaatatt cacgacatgt gccagcgatt ttgccagccg cagctcgtag
    36061 cgacgcaatg ccagttccgc taccggatcg ctttcttcaa ccaggcggat aatttcactg
    36121 cctttcagcg catgtccgct caaacgacga taatctgtgg caaatcccgt gcccgaaata
    36181 aaggtttcaa tacaaccttg tttaccgcaa taacaaggga cttcctcgcg ataacgcagt
    36241 tcgtcttcgt ccatccacgg tagcggattg tgtccccact cgcctgccgt gccattgccg
    36301 ccgatatgcg cccgcccatt gaatgccacg cccgcgccgc atcccgtgcc gataatcacg
    36361 gcaaataccg tctgtgctcc cgctgccgcg ccatctactg cttctgaaac ggccagacag
    36421 ttggcgtcat ttgccagccg cacttcccgc tgcaacctcg cgcttaagtc tttatcgaat
    36481 ggctgaccgt tgagccaggt tgaattggca ttcttaacca caccggtgta aggcgaaatt
    36541 gagccaggaa tgcccatacc taccgttccg cgctgccctg tcgcctgctc cgccatatca
    36601 accaacgtgg cgatcgtttc aatagtctgc cggtaatcat cacgcggcgt gggcagacga
    36661 tggcggtaca actgctcccc tgcatcgccc agtgcaatca cttcagtttt ggtgccgcct
    36721 aaatcgatac ctatacgcac ggtactctcc ttattttttt caatatcaat agcgtagaga
    36781 cggacaaccg gattggcaat gcaaggccgc cgacaattcg ttatcatgcc cgctaaattt
    36841 aacgacaagg ccgtggaaat tatcatgctg tggttcaaaa atttaatggt ttaccgtctt
    36901 agccgcgaga tttcgctgcg tgcagaagag atggaaaaac agctagcctc gatggcattt
    36961 accccatgcg gcagccagga catggcgaag atgggctggg ttcctccgat gggatcgcac
    37021 agcgatgcgt taacgcacgt tgccaatggt caaattgtta tctgcgcgcg caaagaagaa
    37081 aaaatcctcc cgtctccggt gattaagcag gcgctggaag cgaaaatcgc caaactggaa
    37141 gcggaacagg cgcgtaagct gaagaaaacc gaaaaagatt cgctgaaaga cgaagtgctg
    37201 cactctctgt tgccgcgtgc tttcagccgt tttagccaga caatgatgtg gatcgacacg
    37261 gttaacggtt tgattatggt ggactgcgcc agtgccaaaa aagcggaaga tacgctggca
    37321 ctgctgcgta aaagcctggg gtcgttaccg gttgtaccat tgagcatgga aaacccgatt
    37381 gaactgacgt tgaccgaatg ggttcgctcc ggtagtgcgg cacagggctt ccagctgctt
    37441 gatgaagccg agctgaaatc gttgctggaa gatggcggcg tgatccgcgc gaagaaacaa
    37501 gatctgacca gcgaagagat caccaatcac attgaagccg gaaaagtggt gactaaactg
    37561 gcgctcgact ggcagcagcg cattcagttt gtgatgtgcg acgatggttc gctcaagcgt
    37621 ctgaagttct gcgacgagct gcgcgatcaa aacgaagata tcgaccgtga agatttcgcc
    37681 cagcgttttg atgccgattt catcctgatg actggtgaac tggcagcgtt aattcaaaac
    37741 ctgattgaag gattaggtgg cgaagcacaa cgttaattgc tgattttcct ttaatgccgg
    37801 atgcgacgcc tgccgcgtct tatccggcgt acgaagccac accaggcata taattattcg
    37861 ctacggcggg caataatttt tagcgcagca atattatgcg ttttatgctg taacttgctc
    37921 catggacgtt gtgtcattgt ttttcctcaa gccagtgacg cgcttcactg cgaattttcc
    37981 gcattgcccc ggttttcact aaatcccgta acagcgaaag atcgggttgt tcgacaaata
    38041 agagtaatga tggatcctct tttaaccacg tcgttttcac atcggcagcg agctgtggat
    38101 tattcatagc tagcgaccga tgccgagact cggtcaatgt cgttaacaac gacgccgggg
    38161 tatgtgcgtt gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac
    38221 gccaccattc atcatctgat ttatctttta tcgtagccag ttccgcaaca atatctggag
    38281 atcttcactg atatttatat caaccttgat gaattatgaa tataccggaa attgaaaaaa
    38341 tcgcagcaaa ggaaatatta ttatgggtta aagagaggat atttcagaaa aatgccctcc
    38401 cgttcaaggg gaaggcgagg aattacagat agcggcacag ataagaggtg ggttcggcaa
    38461 cttgcagatg aaactcactg tgaccgggaa cattaaacac cgaaccggct tcatacacct
    38521 gccagtcggt cgcgtcaggc agtaacacat tcagcgcgcc actgattacc gtcatttctt
    38581 ccggctcagc ggtgctgaag gtgtattcgc cttcaaccat aacacccacg ctggcgcgac
    38641 cagtgctgct gctggaaaag ccgattgatt tcactttgcc ggaaaagtac tcattacttt
    38701 gaagcataaa ctggccctgt ttggtagttg aacatctact tcactatagg ggccagaggc
    38761 gctggctgtc acgcaaaatt acaccagtaa ttccgcagcc agccgtgcaa tcaatacgtt
    38821 agacagcaag acaggaacat cgagctgttt ttgcagtaaa tcgcgatgac gttggtgaaa
    38881 tcctaaacaa tccagcatga tgacatcagc cccttttgcc agtaattctt tcccggcatc
    38941 aatgattttt tgttctgaat catgaatggg gttacccaat gaaaataccg gcgatttctg
    39001 caaaatttgc catttttgcg cctgaacggg cagcatctcc tcaaccggaa cgataacccc
    39061 cacctgatga tcttcaacaa tagaggaaac cagtggaggc aatattcgcg acggctcaag
    39121 aaagatcgta ttacgcgcag tcatactact aatgtttgct gtactcatta ataaaatgac
    39181 gtcataaccc tgattatcga gcacttcaac cacaccttgc aggtctcgct ccactttacg
    39241 acgcgaaaca tgggccagct ggatgtcatt taataatgtg agaatggtgt cttcgcctgc
    39301 ttctggcgcg tactccgcca tcacttcttc acgacttaac ttccccagca ggctatgatg
    39361 ggaaatatta tcttcgtcaa tgtattccgt caggagcggc aaaacctcct gcataggtac
    39421 aatgccgatg gtcaggatcg ccaacgacgc actcattgtt catccacctt acttttcttt
    39481 cgtcgttaat taccgggcaa gtgtgaagca ccactgctga cacttacttg tgctacaaat
    39541 gacaaaaagc gtagcagcag acgtgcacgg cataatgtaa agattccaaa tgattccagt
    39601 aatggattgt tattgtttaa tattctaatt attagaaaaa catgaattat gaaaaatgtg
    39661 acgcagatcc cttgtagtgg cactcagaat cccttccgag cagtctgatg cggtcactct
    39721 ttgtcagggt cctcatagcg ttttttcgca tccatcgctt cctgagccgt cggatgttca
    39781 ctgatcaacg agtctggttt aggatgatcg gctctgagtt ggtaccaggt taccgtctgt
    39841 cctggctttc ctttttcaat cgtcactata tatgcttcac gaggataagg cggtttcgtt
    39901 ggcatcgttc ttccttattt cacgggatga acgttaagta taggcgctct aaaatcaaca
    39961 attgatcgtc tgtgccaggg cgctacgaat ttcagaaatc acctggctgg gttcgtttgt
    40021 tgcgtcgatg ataatatgcg caacttcgcg atatagcgca tcgcgttctt ccagcacttc
    40081 ctgaacttct tcgctcagcg gttttcccgt taaggttggc cgtaaatctt cttccggtgc
    40141 agcttgcagt cggttaacca ggactgatac tggcgcacac aaataaacca cgatcccgtt
    40201 attttgcata aagtgacgat taaattccgt cagaataatg ccgccgcctg tagcgataac
    40261 ggtggatggc gcagttaccg cttccagcgc cgccgtttct ctggcgcgaa atcccgccca
    40321 ctcttccctt tcgacgatct ccgcgaccgt catattgagc tgtgattgca accactgatc
    40381 ggtatcgaca aaccgacggt taagcgaatc ggcaagggcc attccgaccg ttgttttacc
    40441 acagccccga ggcccgatca gaaaaagagg ttgtgtcatc gtgggttttc cccaataggt
    40501 cgcaatgcgg cgaaagccgg tgtcatgaga atagcgatca taccatcaaa ctagtacaat
    40561 ttcgattgta aagaaaaaat tccacttaaa gtgaaaatct caatacatcc cttactatac
    40621 caataaatat tcaagaatga agtgtaaaca ataaattaca tttagccacg actacgttgc
    40681 acttccagcc accacttctc cagctccgcg gcaaaggcct ggcggtcacg ttgtgaaagg
    40741 ctatctggtc cgccggtctg gatcccactg gcacgtaagg tatccataaa atcgcgcatc
    40801 gtcaggcgct cacgaatggt cgctggcgtg taacgttcgc cgcgcggatt aagcgccgct
    40861 gcgcctttct cgatggcttc agcagccaaa ggtatatctg cggtgatcac caaatcgccc
    40921 gcttcacact gccggacaat ttcgttatcg gcaacgtcga aacctgccgc gacgcgcagc
    40981 gtacgaataa atcgcgatgg cggcacgcgt aaactctggt ttgctaccag taccagcggc
    41041 atctgcatac gttccgccgc gcgatacaaa atctctttaa ttacactggg acacgcgtcg
    41101 gcatccaccc aaattgtcat aaagtcatcc tttgttgggt aatcctctat tgtgtcgcgc
    41161 ttttgccttc cggcatagtt ctgtttatgc ttctgccagc gattatcaaa acaatgaatt
    41221 tcacggcagg agtgaggcaa tggaaaagaa aatcggtttt attggctgcg gcaatatggg
    41281 aaaagccatt ctcggcggtc tgattgccag cggtcaggtg cttccagggc aaatctgggt
    41341 atacaccccc tccccggata aagtcgccgc cctgcatgac cagttcggca tcaacgccgc
    41401 agaatcggcg caagaagtgg cgcaaatcgc cgacatcatt tttgccgccg ttaaacctgg
    41461 catcatgatt aaagtgctta gcgaaatcac ctccagcctg aataaagact ctctggtcgt
    41521 ttctattgct gcaggtgtca cgctcgacca gcttgcccgc gcgctgggcc atgaccggaa
    41581 aattattcgc gccatgccga acactcccgc gctggttaat gccgggatga cctccgtaac
    41641 gccaaacgcg ctggtaaccc cagaagatac cgctgatgtg ctgaatattt tccgctgctt
    41701 tggcgaagcg gaagtaattg ctgagccgat gatccacccg gtggtcggtg tgagcggttc
    41761 ttcgccagcc tacgtattta tgtttatcga agcgatggcc gacgccgccg tgctgggcgg
    41821 gatgccacgc gcccaggcgt ataaatttgc cgctcaggcg gtaatgggtt ccgcaaaaat
    41881 ggtgctggaa acgggagaac atccgggggc actgaaagat atggtctgct caccgggagg
    41941 caccaccatt gaagcggtac gcgtactgga agagaaaggc ttccgtgccg cagtgatcga
    42001 agcgatgacg aagtgtatgg aaaaatcaga aaaactcagc aaatcctgat gactttcgcc
    42061 ggacgtcagg ccgccacttc ggtgcggtta cgtccggctt tctttgcttt gtaaagcgcc
    42121 aaatctgccg atttcaacca ctcacgatag tgactcattt gtgggttcag cggcgcaacc
    42181 cccacactaa tccgtaaagt tacctgtggc gtattcggca agcgtaacgt gtttagccct
    42241 tcacgcaccc gtaacatggc ggtaatggcg ctctcagctg gcgtaccgga catgattact
    42301 gcaaactcat cgccgccaaa ccgaccaatc acatcgctac cgcgcagggt aatttgtaac
    42361 tgtcgggtaa gcgccacaat cgcttcatcg cccacatcat gaccccaggt atcgttgatg
    42421 ctcttgaaat ggtcgatatc gataatcagt aacgttgcat cgcgattatg ccgccgacag
    42481 ttatcaaatt cattgcgtaa catagtttcc caatgacgtc ggttatacac gcccgtcatg
    42541 ccgtcgcggg tactcatgac ctgcaacctg cgtttatgtt ccgccagttt ggttgccgtc
    42601 tggtagctga cccagccaaa cagcagagga taaacgacaa taatgggaag ggagagccac
    42661 cattccagcg gcgcactatt gaacgacacg gtaatgcccg taagctcgag ggtgacaagg
    42721 caggaaacca ccatcaacac cagacccgcg acaaacagac gggggccgcc tgcccccatc
    42781 aaattcagac acataatcat caacatagcg gtggaaggca gcacgtttac gcccattacg
    42841 cctacccaca ttcccgctaa tactgcatcg gtttttaagt tgtaaatttc ccggctaagc
    42901 ggatcgacgg ccctgctcgc tatctgccag gctaaatgcg gccagacgaa cgcccagccg
    42961 accaacacca gccaccacca gcccggcggc gggtgcgaaa ccagcgttga agcaatcggt
    43021 aagaacatgc cagccaggcc aaccgcacgg ggtagtctga cgcgacgggc gaagcgcagc
    43081 ccggaccgct gatgttcgtt ttgaggagtt aaaggaggtt cctccccatg cgccgccgct
    43141 tttttgaaaa agttttcatc attcattatt tttgggaaca ttcagaaaca attttcccaa
    43201 attatagaga cggggctaaa ggcagaagta tgaaatttcg ggtgagccac tttcgtagct
    43261 cacccgttag cactatcagg tatcaggcgg ctttcttgag gcaggcactc ataaacttat
    43321 tacgatcatc acctttcaga gattgttgcg ttgcttgatt attacattcg cgcatctttt
    43381 gctgctgtgg cgtcaaactt ttttcaccag gcgcagactt gctgttcttc aggcaatcac
    43441 tcatgtaggt cttacgagca tcccctttca acgcctgcgc cgtcgcctgc tgattacagg
    43501 aggtcatacg ctgttgttgt ggggttaagg ttttctcggc agcgccgacg gtggttaaaa
    43561 aaaccagacc aaaaagcaag gtaaccagta atgttatttt catagcacca tccctcttca
    43621 tgttttaacc atgagcgttt gcgcccgtga tctgccatta agtctggttg ctaacagcaa
    43681 aaaaaccacc cggcagcgaa aattcactgc cgggcgcggt tttatttcag ccccagagcg
    43741 gctttcatgg tgtagaagag atcggtctgg tcggtcagtc caacaacatt ggcggcatgc
    43801 gggccatacg ccgcaatacg caactgactg ccggtatgtt cttgtgaatc ctcttcagag
    43861 ttcccgtaac tcatcaccat cactgcgcca tctttagtat ttagcgcctg agtgaggccc
    43921 ggcgctttgg tatccggcgc aacaacctgg ctggcgtggg cgtgatcagc ggtgactatg
    43981 accagcgtgt taccctcctt tttagcgaat tccagcgccc gttgtacggc ttcatcgaga
    44041 tcgaccgtct cgccaatttg cccacaagga ttcgcagcgt gatcctgttt atcgattgac
    44101 gcaccttcaa cttgcaggaa aaatcctttc tcatttttac tcaacaattc aatggctttg
    44161 tcggtcatct gtgccagggt tggtacactg tcattacgtt gcggattagg cgtacaggtg
    44221 actgcgggct tatcgatatt gccgtggtac gttgctttcg gccctagcca gcgcactggc
    44281 atattgccgt cagcaaacag gccaagcagg ggtttttgct gattcgcttc cgtcaccgaa
    44341 ttcagtgagg cagcatcgtt caccaactga taaccacgcg cctgtgcctg ttcacgcagc
    44401 gtttttccct gccattcacc agcggttgcc gtttcagcaa aggtttttgc gccgccgcca
    44461 agcgtaacgt cggcacgagc gttaagcagc tgttcggtaa tcgatccttt tccgcctttt
    44521 tccagagcgt tacccggaca tttttcactg gtcgcgctcg gaccgtagca tttgcgcgaa
    44581 gtcacatgtg ccaccagcgc agcgggcgtg gcatcctgca actctgcggt agaaacgtta
    44641 ccggtcgcca gacctgcggc ttttgccatt tccagaatcg ttgggtgatc tttttcgtga
    44701 atatcgacgc ccagcgcgcc gttataggtt ttgacaccgg ttgaccaggc ggttgctgat
    44761 gcagccgagt cggtgacgta gtccggtttg ccagtttttt tattcagcgc atagtgagtg
    44821 tattgcccgg taagcggtaa ggcatcgata cctttaaaaa agccgcccgc accttcggca
    44881 taattacgtg cggcagtaat ttccgaatcc cccatcccat cgccaatcag caaaataata
    44941 ttttttgcag gtttatcgct aagagaatca cgcagagcgg cggtatgatc acccgttaag
    45001 cggcgagcac cgccgggtgc agtaatatcg ccctgagcag cccggttttc cagaacaggc
    45061 atttctggag tccgggcttt tgtcacaggg gtaaacagta acgttaagag tgccagtgca
    45121 atagtgcttt gtttcacttt attttctcca tgtacaaata cattaaaaaa taaaaacaaa
    45181 gcgactataa gtctcggccg tgacaacttt atgacagctg ttgaaaagat taacttttta
    45241 ctgacgagga tgcttcaata acttctttac gtaatcgcgc agcagctccg tatcgtcgtc
    45301 aggaatgctg gcatcgggct ttacctcgta cagcgccccc tctacctgat caatcaaccg
    45361 ctgttggtca ttttgcgcca tattgcgaag cattgcagtg acgataatct ccaaagcttc
    45421 tacctgcgca cacagttctt tcgactcttc ttctttttgg gcaagcttaa ataacaactc
    45481 agcaatgaga tttttcatgt ctgtatttcc ttatccaaag tatggagaag ttaacattat
    45541 gtttcacaac ttcatagtgt ctggaagtat tatttttatg acaaaggaaa tactttgcgc
    45601 aaatattttt tgtttgatta ccagcgaaac gtttctctta tttaaataat tacccacaca
    45661 aaatataggc ttttaattat caacctttat tataaataaa agttatgaaa tatattctct
    45721 tcgttaactt ataattaaga gaaaaaaact caccgcatgg ccatttaagc caggaataaa
    45781 ttagcttccc gttcatcatt tttgtgatct tgcgcacatc tttgagataa cgcactttct
    45841 tcaccagtac gttgcacgct ttgtcagcag aagattgcaa caatctttca gaaggccgcg
    45901 aatcccccgt tttgtcgtgg taatgtatgc actttgtgtt agacgggata ggtttttaag
    45961 atggaaaaac tgcgggtagg aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct
    46021 ctgcaatcgg caaaaaacat tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg
    46081 ctgggcattg ataaacaagg gcaatggcac gtcagcgatg ccagcaatta tctgctaaat
    46141 gcagacgatc ctgcccatat tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca
    46201 ggtaaacatg agcatcagct tatcgacgcg caaaacggtc agccgttgcc gacggtggat
    46261 gtcattttcc cgattgtcca cggtacgctg ggcgaagatg gttccttgca gggaatgctg
    46321 cgggtcgcca atttaccgtt tgtaggttct gatgttctgg cttcagcagc ctgtatggat
    46381 aaagatgtca ccaaacgtct gctacgcgat gccgggctga acattgcgcc atttattacc
    46441 ctgacgcgcg ctaatcgtca caacatcagt tttgccgaag tggagtctaa actggggtta
    46501 ccactgtttg taaaaccggc taatcagggc tcttctgttg gtgtcagcaa agtaaccagt
    46561 gaagaacagt acgcaattgc cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt
    46621 gagcaaggga tcaaaggtcg tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa
    46681 gccagcacct gtggcgagat cgtactcacc agcgatttct atgcctacga caccaagtac
    46741 attgacgaag atggcgcgaa agtggtagtt ccggcagcca ttgcaccaga gatcaacgat
    46801 aagatccggg cgattgccgt tcaggcttat cagacgttgg gatgcgcagg catggcgcgt
    46861 gtagacgtgt ttttaacccc agagaacgaa gtggtgatca acgagatcaa cacactgcct
    46921 ggcttcacca acatcagtat gtatccgaag ctgtggcaag ccagcggtct gggttacacc
    46981 gatctgatca cacgtttgat tgagctggcg ctggagcgtc acgctgcgga taacgcattg
    47041 aaaaccacaa tgtaatattt taacgcccga taatattctt attgggcgtt ttcagtttct
    47101 tcatcgcgac gacgaataat caaccccgcc agccagaaac taatcaccca cgtcaccaga
    47161 ccgacggcat aggtctgcca accttttgct tcaaatccca gtaaccccac cacgccatta
    47221 agaatgaata taaggccaat ggcgaacgca tagtagtgcc agtcacggcg gatttttaca
    47281 ggcagattca tggcagctcc gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc
    47341 gtctgtggtg tagacaacat tttcgtaatt gttaataaat ttaacttaag gttgccattt
    47401 tgtgactcac aacgaagatt cacgcttcgg gaatattccc aacatcaatt taccatgatt
    47461 ctgatattga caatctaaat gacctgtcag acttgccata agccgctggt atcacaccag
    47521 aagagaagca gatgacttcc ggaggttttt atggctgatt tcaccctgtc aaaatcgctg
    47581 tttagcggaa aatatcgcaa tgcctcttca acgcctggca acattgccta tgcgttgttt
    47641 gtgctgtttt gcttttgggc tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc
    47701 gtctatgagc gtttaatgca ggtccaggaa acaggtcgcc cacgggtgga aattggttta
    47761 ggtgtcggca ccattttcgg gctgatcccg tttttagtag gctgcctcat ttttgcagtg
    47821 gtggcgctat ggctgcactg gcgacatcgc cgccagtaag atttacgttg ccagatacgg
    47881 cctaaaacgc cttatctggc atgacattcc tgtttgatca ctgtttcata caacgcgccc
    47941 gccgttcgtc tacgcgcttc gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc
    48001 tttccagttg aatgccaggc aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg
    48061 caagttgata aactttcttg tacagcgccg tctcctcaaa agagaagctg tcacctttct
    48121 ctaactgacg gcgaatttcg ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg
    48181 ccagttcggt tttcccgggt tctttactgt cgtaacgaat taaatcaccg tccagtgcca
    48241 gcttcacgcc gctggcctta ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc
    48301 agccagcgtt aaaatcagca aagcggtata tcggtgcgct atagctggcg ggataattca
    48361 gtaaatggta agtaccaaac cacaacccgc cgcggcgact gaagacttcc tgacgaactg
    48421 taccgtccat tttccacgga taccctttgg tatgctgttc ggcaaaagca atgctgacct
    48481 gcatcggccc accggtgcgc acaggattaa gcgaaccaaa cagcgtctgc cccattggta
    48541 ccatgctgat cagatcgtca aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta
    48601 accgttcgct ataacttttg ccgtttggcg atttgatttt tagcgcggta tgaaccagaa
    48661 aagcaggaat gtgcatccgt tcagcacgac ggtcaatctc ttgccaggcg atcttgctta
    48721 agcccggaac ggccggatct gcctgataat ttgactcctg ctgcgccacc gccagcaccg
    48781 agcagacgtt ttccagcgtc ggtgccagcc cctgactttc aaaggtggtg gcgagatctt
    48841 tcgcccaggc atcgcggtct ttcacgctcg caggcatctt ctgccgcacg acgctcgcca
    48901 catctatcgc tttttcgccc tttttcaacg gttgcggagc ctgactacta caccccgcta
    48961 aaaccagaac cgccaacaat gacagcgaag atagaggatt gacgcgcgac attacttctc
    49021 ctttttagct caaggtatgg gttacttcct gaattttgtc accatccagt tcatgttcaa
    49081 agctgcgcag acgtttgtag atagacatca actcaaccag tgtggtccat gagttaatca
    49141 ggtactggaa agcaccgcga acctgaccaa aaacgttggt aatctgcgtc atcaggccga
    49201 gcgtaatcgt accggcaaca attgacggaa acagcaagaa caaaccgaaa acgttatcga
    49261 cctgcaaata gagaatgcgg gcgatgttga aatacatata gtgaaaatag aggcggaaat
    49321 agtttttccg cacggcgcta aacagctcgc gtaccgtagg cggcgtcgca cgcgtggcat
    49381 cgtcttcacc ataaaccagc tctttacggt aggcggcctc tactcgctgg tttttaaact
    49441 ccagccccgg cagtttgatc cctaccactg ccagcaatcc ggtccccatc agcgaccaga
    49501 cgatagcggc aatcaccaga ccatacggaa tgtgcccgat aatcggcagc tccggcacat
    49561 gcgtggagag cgttaccagc accggcagga aggcgatcaa cgtcatgatg gcgttgataa
    49621 aactgacgcc catattctcc agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct
    49681 gtgcggcccc ttcgatatga cgcagttgtt gccagttcgc catgtaatat tcgttcatcg
    49741 ccgtacgcca gcggaacacg tagtgactga caaagaagtt gttcagcaca ctgatcacca
    49801 cagcgatcag cgcaatcccc agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga
    49861 ctttatgcgg cgaacttagc gcggtttgaa tcagatcata gaacggtgca taccaggcgt
    49921 tgacggcgac cccgacttcc accaaaaacc aggtgacgaa gatgatcagt gcagtaccga
    49981 gtattgacca gtattgccaa cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc
    50041 ctacgcaaac aatgtagtaa gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg
    50101 cgctaatcgg gatctgcccg gaagcgccag tgatacgcgc cacccactct cccccaccgg
    50161 cttgccagaa gataacggca atcaatgccc aaacaaaggc cgagagaaaa aacgctcccg
    50221 gctttgggaa aaaagactta aacatatgca ctcctgctaa cttcttatcg ttttaacaac
    50281 tgctgtgtat taaccgcatg accgctacaa agcgtcagcc aaatgaaacc gcccattgtg
    50341 acccatagca aggcgcttag ttcgccagcc tgaccataag aattgtttcg acggatttca
    50401 caaaccaaag attgtttaca taagcggatt aacacgcgca ggatcacaaa tgcaggcatt
    50461 ggtgagcagg aaaaagatcg tgtttacgcg ccgttacgtt tttaacacta ttattgcact
    50521 aattcgccct ttgcaatcca tcaatgagta gtataaatac gctcagttac cttcattcaa
    50581 tctatggaca ccaccgttga aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc
    50641 attgacctcc gtccatgcgg cgcagccgat cactgaaccg gagtttgcct ctgatattgt
    50701 cgatcgttat gccgatcata ttttttacgg cagcggtgcc acggggatgg cgctggttgt
    50761 tatcgacggt aaccagcgcg tctttcgcag ttatggcgaa acgcgacctg gtaataacgt
    50821 tcgcccgcag ctggattcgg tcgtgcgtat tgcttccctc accaagctaa tgaccagtga
    50881 aatgctggtg aaattgctcg accaggggac cgtgaagtta aacgatccgc taagtaaata
    50941 tgctccgcca ggcgcacgcg tgccaaccta caatgggacg cccattaccc tagtgaatct
    51001 ggcaacccat accagcgccc tgccccgtga acagcccggt ggcgcggcac atcgtccggt
    51061 gtttgtctgg ccaacgcgcg agcaacgctg gaaatacctt tctacggcga agctgaaagc
    51121 cgcgccaggc agtcaggcgg cgtactctaa ccttgcgttc gacctgctgg cagatgcact
    51181 ggcgaatgcg tccggcaagc cttataccca gttgtttgaa gagcagatta cccgtccatt
    51241 ggggatgaaa gacaccacct acaccccctc accggatcag tgccgccgtt taatggttgc
    51301 tgagcgcggt gccagtccgt gcaataacac gctggcggca attggcagcg gcggcgtcta
    51361 ttccacgcct ggcgatatga tgcgctggat gcagcagtat ttgtcatctg atttttatca
    51421 gcgcagtaac caggcagatc gcatgcagac gttgatttat cagcgcgcgc agtttacgaa
    51481 agtgattggc atggatgtcc ccggcaaagc ggatgcgctt ggtttaggct gggtatatat
    51541 ggccccgaaa gagggtcgtc cggggattat tcagaagaca ggcggtggtg gcggtttcat
    51601 tacctatatg gcgatgatcc cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc
    51661 gccgttgacg cgctttaaaa atatgagcga tggcatcaat gatctggtga ccgagctaag
    51721 cgggaataaa ccgttggtta tccccgcatc ctgattaata ctctgaaggc aaacggttga
    51781 tagcgaccag tttgccttta ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa
    51841 cttctgcgac gacagaacgc gatatgcgtg tacgttgatg gatataattc atcacaccaa
    51901 tacgtgatcg caattcttca ttccagtcaa tcattgataa taatgttgcg cggatttgtt
    51961 cgtaggaatt atgcccaatg agctgcacgt cgcgtaattc cagaattcta ttttgccagg
    52021 ctaaccagta aaaagcgtct cgccagagtt gattttgttc aataagcgta atggtttgtt
    52081 tggctggtag atgatatccc gtacaatttc cttctgatat taatttgtat ggtatatcgt
    52141 ttttcattaa accatcagcc agccccataa tataaggagc ctgggtaata ccgataagta
    52201 cgttttcttc tctacgcaga gagataacgc cctctaaaat aacgaaagta tcttcgttat
    52261 tgacatcact ggaaaatata acttgctttt cattattaaa ctcgaagcgc gtaccgtatc
    52321 tggacaaaca tttatcgagc ttaccaaatt cctgaagagg tttaactaca gataacattt
    52381 gcgcgtcctt tgcagtaatg cccgtcaaat ccttgacggg cattatttag attaaattac
    52441 cagaatttct tcggagtgaa gaatattacc aggtatattt aacacccacg ttcgcggacc
    52501 agtcttgatc tacgtcacca ccaccgaggt agttagcatc ggtataggcg ctgaagttct
    52561 tggtgaagct gaactgagta cccagaccaa cacgtaccgc agacccttca gtaccgttat
    52621 cgatggaatc accgttcaca tcgttatcgt tgttagagtc gtcgtagacg taagccagtt
    52681 tgaagtacgg agtcagagcc tggtcttcgc tgtaggtgaa ggtataacct gcatctacac
    52741 ccagttcata acgcatgctg tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct
    52801 ggtagtcatc accagactgg aacagaccag aaatgctgcc gtaaggagtc acgtaaccag
    52861 catcacccag tttgaagtcg taaccggctt tcaaaccgaa gccccaagcg tcggagttgg
    52921 tgctaccgtc aacgtaagta ccgttgctca tggttgcaga caggtcgttg ttgaagtgag
    52981 agtaactcaa gctaccatca acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt
    53041 aggcagtctg gctgtcttga tccacctgac cagaacggtc attcatgtca cctttagcga
    53101 agcctgcagc cgcaccgacg atccacttag cgttgttacc gtcaattttg gtatcaacac
    53161 cgaccatgat gccgttaaca tcctgatcat agttgatggt gccgttgtcg ccgttgaagt
    53221 taccaccgaa gtagcttacc catgcgccgc cgttatcagc caggccatga cgagagttgg
    53281 tcagacgagt accaacggtg tcttgttcca ggttccagat attggtgttc gcagacggga
    53341 tgctcagcgc catgttagcg tagtcggtca gctccatctg ttgcagaaca acggtgttac
    53401 cgcgctgttc agcctgatag gtgtatgcac ccaggtcagc tttgttagca gcagagaagg
    53461 tcgcgttggt gttgacgtcg tttacgtaga taatttcttt atctttgtaa tcagcgatag
    53521 cgccagcgcc tgttgcgttg tcgatacgaa
//
LOCUS       DAZFXT010000015        53180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.015, whole genome shotgun sequence.
ACCESSION   DAZFXT010000015 DAZFXT010000000
VERSION     DAZFXT010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 53180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.015"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(119..835)
                     /gene="yigB"
                     /locus_tag="ACHKW0_001036"
     CDS             complement(119..835)
                     /gene="yigB"
                     /locus_tag="ACHKW0_001036"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HFO7473913.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(835..1731)
                     /gene="xerC"
                     /locus_tag="ACHKW0_001037"
     CDS             complement(835..1731)
                     /gene="xerC"
                     /locus_tag="ACHKW0_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HFO7473914.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(1728..2435)
                     /gene="yigA"
                     /locus_tag="ACHKW0_001038"
     CDS             complement(1728..2435)
                     /gene="yigA"
                     /locus_tag="ACHKW0_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HFO7473915.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(2432..3256)
                     /gene="dapF"
                     /locus_tag="ACHKW0_001039"
     CDS             complement(2432..3256)
                     /gene="dapF"
                     /locus_tag="ACHKW0_001039"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HFO7473916.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(3293..3496)
                     /gene="yifL"
                     /locus_tag="ACHKW0_001040"
     CDS             complement(3293..3496)
                     /gene="yifL"
                     /locus_tag="ACHKW0_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7473917.1"
                     /translation="MKNVFKALTVLITLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            3846..4400
                     /locus_tag="ACHKW0_001041"
     CDS             3846..4400
                     /locus_tag="ACHKW0_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3304 domain-containing protein"
                     /protein_id="HFO7473918.1"
                     /translation="MGLFTQLEKFDQKLTRGYARWGRWVWRTLIVVPVLVVLWNIGQA
                     VWGGPRGGVILEIHSEIDRPILGFSVNGVAGANAFANGGGSTTCCGDVSGDTAEVIWT
                     LSTTRTQYNAGMRLEKRNMTLPLPKREWGEDFLHVHFMPGDKVLLGWSKDSFSPYEDL
                     HNGGYKTRVRQDVKDKLYGTGKMN"
     gene            4738..6246
                     /locus_tag="ACHKW0_001042"
     CDS             4738..6246
                     /locus_tag="ACHKW0_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO7473919.1"
                     /translation="MIGTSRQDGFMHYRNVVCLLSCSLFLSSAWGCRLDPPEHNIYQK
                     QGKGVVYLRPYKETNLSLPDVNYKSLRRLPNLLIDPTTLDKWDKVPPLTDLTTDYLYE
                     GAQAWYPHYSWHSDGRYILYAGEVVQNPPDKPPVDVASFKAWGDFAADKHSLYFEGKR
                     TDNNGGGNRLDIKTLHQVEFRPPWDPDLLGLILRDANFLYINGHRLADPDSFRVLAQK
                     SWDQRGKFSTAFNPCVAMPFGPWDTLARTRTKILINGEQLDADPDTFSVVRWMPGSLL
                     TWRDKNGLQRKVLDKENLEWDEDLTKHCLDFSLLEKKVFWRKGPACKQEELPGLDPEQ
                     FQPISDAVAQYQDSLYTIIETESGDRKLEIVKLDDPNLIINKRFNAGKRHGYLLTRAE
                     GWPYHSGLHVFESDGPLILLDNRSPDEREAHLNDHPFLRRWYARDNRYIYSFDGAQLW
                     RYRTADPKQVRLIWKEQHSGYGYGVNYKTGYLDGKITDDGEFIPAPRNEATK"
     gene            6243..7568
                     /locus_tag="ACHKW0_001043"
     CDS             6243..7568
                     /locus_tag="ACHKW0_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HFO7473920.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPKMNRADLRLVTNISGERELVEGYIPTDEISIKNEEYHWVTDGRVILWRG
                     KIVSNPPGTPTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVDLATLKAIEGNS
                     TTLVDSKNLYLSGRRQGSSSNVTVLEKRWWGINPRLMSVNRNLYSNDLLIRSGQNIYL
                     NGVHLTANADSFEIIRWIPHSLLVFRDNKGMHRYPFGQLSGKAIPVDDDVSFEVGESR
                     VRWRKQLTSDRQWSKWIDLPDIEPEQFHLITGNIAQYKDRLYVTKLSTFGEDQLEIIP
                     LDTPDLVIDRSFNSGKQHAYFIRQLRSKSVQIIPVNGPLTKNDRFAYDDRNVYTWTDT
                     EVRITPSPCPAKTHVREENVRELHNRDIIIPLTDDSCRNAATDGQTLKP"
     gene            7616..7936
                     /gene="cyaY"
                     /locus_tag="ACHKW0_001044"
     CDS             7616..7936
                     /gene="cyaY"
                     /locus_tag="ACHKW0_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HFO7473921.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(7976..10522)
                     /gene="cyaA"
                     /locus_tag="ACHKW0_001045"
     CDS             complement(7976..10522)
                     /gene="cyaA"
                     /locus_tag="ACHKW0_001045"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0006171 - cAMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HFO7473922.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            10909..11850
                     /gene="hemC"
                     /locus_tag="ACHKW0_001046"
     CDS             10909..11850
                     /gene="hemC"
                     /locus_tag="ACHKW0_001046"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HFO7473923.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            11847..12587
                     /gene="hemD"
                     /locus_tag="ACHKW0_001047"
     CDS             11847..12587
                     /gene="hemD"
                     /locus_tag="ACHKW0_001047"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HFO7473924.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            12609..13814
                     /gene="hemX"
                     /locus_tag="ACHKW0_001048"
     CDS             12609..13814
                     /gene="hemX"
                     /locus_tag="ACHKW0_001048"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HFO7473925.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFIAIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPQGD
                     TPAAAPQGE"
     gene            13817..15013
                     /gene="hemY"
                     /locus_tag="ACHKW0_001049"
     CDS             13817..15013
                     /gene="hemY"
                     /locus_tag="ACHKW0_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0042168 - heme metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HFO7473926.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            15691..15828
                     /locus_tag="ACHKW0_001050"
                     /pseudo
     CDS             15691..15828
                     /locus_tag="ACHKW0_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312758.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
     gene            complement(15936..17171)
                     /gene="aslB"
                     /locus_tag="ACHKW0_001051"
     CDS             complement(15936..17171)
                     /gene="aslB"
                     /locus_tag="ACHKW0_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7473927.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(17318..17394)
                     /locus_tag="ACHKW0_001052"
     tRNA            complement(17318..17394)
                     /locus_tag="ACHKW0_001052"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17358..17360),aa:Pro,seq:tgg)
     gene            complement(17437..17523)
                     /locus_tag="ACHKW0_001053"
     tRNA            complement(17437..17523)
                     /locus_tag="ACHKW0_001053"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17487..17489),aa:Leu,seq:cag)
     gene            complement(17544..17619)
                     /locus_tag="ACHKW0_001054"
     tRNA            complement(17544..17619)
                     /locus_tag="ACHKW0_001054"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17584..17586),aa:His,seq:gtg)
     gene            complement(17678..17754)
                     /locus_tag="ACHKW0_001055"
     tRNA            complement(17678..17754)
                     /locus_tag="ACHKW0_001055"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17718..17720),aa:Arg,seq:ccg)
     gene            complement(17857..19242)
                     /gene="yifK"
                     /locus_tag="ACHKW0_001056"
     CDS             complement(17857..19242)
                     /gene="yifK"
                     /locus_tag="ACHKW0_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7473928.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(19433..20173)
                     /gene="wecG"
                     /locus_tag="ACHKW0_001057"
     CDS             complement(19433..20173)
                     /gene="wecG"
                     /locus_tag="ACHKW0_001057"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047241 - lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HFO7473929.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(20176..21528)
                     /gene="wzyE"
                     /locus_tag="ACHKW0_001058"
     CDS             complement(20176..21528)
                     /gene="wzyE"
                     /locus_tag="ACHKW0_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HFO7473930.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(21525..22604)
                     /gene="rffT"
                     /locus_tag="ACHKW0_001059"
                     /gene_synonym="wecF"
     CDS             complement(21525..22604)
                     /gene="rffT"
                     /locus_tag="ACHKW0_001059"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HFO7473931.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFAARGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(22601..23851)
                     /gene="wzxE"
                     /locus_tag="ACHKW0_001060"
     CDS             complement(22601..23851)
                     /gene="wzxE"
                     /locus_tag="ACHKW0_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HFO7473932.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(23853..24983)
                     /gene="rffA"
                     /locus_tag="ACHKW0_001061"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(23853..24983)
                     /gene="rffA"
                     /locus_tag="ACHKW0_001061"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HFO7473933.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(24988..25662)
                     /gene="rffC"
                     /locus_tag="ACHKW0_001062"
                     /gene_synonym="wecD"
     CDS             complement(24988..25662)
                     /gene="rffC"
                     /locus_tag="ACHKW0_001062"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HFO7473934.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGVGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(25640..26521)
                     /gene="rfbA"
                     /locus_tag="ACHKW0_001063"
     CDS             complement(25640..26521)
                     /gene="rfbA"
                     /locus_tag="ACHKW0_001063"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HFO7473935.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(26540..27607)
                     /gene="rffG"
                     /locus_tag="ACHKW0_001064"
     CDS             complement(26540..27607)
                     /gene="rffG"
                     /locus_tag="ACHKW0_001064"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO7473936.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(27607..28869)
                     /gene="wecC"
                     /locus_tag="ACHKW0_001065"
     CDS             complement(27607..28869)
                     /gene="wecC"
                     /locus_tag="ACHKW0_001065"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0089714 - UDP-N-acetyl-D-mannosamine
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HFO7473937.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(28866..29996)
                     /gene="wecB"
                     /locus_tag="ACHKW0_001066"
     CDS             complement(28866..29996)
                     /gene="wecB"
                     /locus_tag="ACHKW0_001066"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HFO7473938.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(30052..31098)
                     /gene="wzzE"
                     /locus_tag="ACHKW0_001067"
     CDS             complement(30052..31098)
                     /gene="wzzE"
                     /locus_tag="ACHKW0_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HFO7473939.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(31110..32213)
                     /gene="wecA"
                     /locus_tag="ACHKW0_001068"
     CDS             complement(31110..32213)
                     /gene="wecA"
                     /locus_tag="ACHKW0_001068"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0036380 -
                     UDP-N-acetylglucosamine-undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HFO7473940.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(32453..33712)
                     /gene="rho"
                     /locus_tag="ACHKW0_001069"
     CDS             complement(32453..33712)
                     /gene="rho"
                     /locus_tag="ACHKW0_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HFO7473941.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(33797..33898)
                     /gene="rhoL"
                     /locus_tag="ACHKW0_001070"
     CDS             complement(33797..33898)
                     /gene="rhoL"
                     /locus_tag="ACHKW0_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HFO7473942.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(34039..34368)
                     /gene="trxA"
                     /locus_tag="ACHKW0_001071"
     CDS             complement(34039..34368)
                     /gene="trxA"
                     /locus_tag="ACHKW0_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HFO7473943.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            34499..35764
                     /gene="rhlB"
                     /locus_tag="ACHKW0_001072"
     CDS             34499..35764
                     /gene="rhlB"
                     /locus_tag="ACHKW0_001072"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HFO7473944.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            35898..37382
                     /gene="gppA"
                     /locus_tag="ACHKW0_001073"
     CDS             35898..37382
                     /gene="gppA"
                     /locus_tag="ACHKW0_001073"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008894 -
                     guanosine-5'-triphosphate,3'-diphosphate diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0015974 - guanosine pentaphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HFO7473945.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(37429..39450)
                     /gene="rep"
                     /locus_tag="ACHKW0_001074"
     CDS             complement(37429..39450)
                     /gene="rep"
                     /locus_tag="ACHKW0_001074"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HFO7473946.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALTVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            39667..40116
                     /locus_tag="ACHKW0_001075"
     CDS             39667..40116
                     /locus_tag="ACHKW0_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HFO7473947.1"
                     /translation="MAINFSPKVGEIQECNFGNYPVIQNGQFSTTYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINVLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            40315..40596
                     /gene="ppiC"
                     /locus_tag="ACHKW0_001076"
     CDS             40315..40596
                     /gene="ppiC"
                     /locus_tag="ACHKW0_001076"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HFO7473948.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(40683..42158)
                     /gene="ilvC"
                     /locus_tag="ACHKW0_001077"
     CDS             complement(40683..42158)
                     /gene="ilvC"
                     /locus_tag="ACHKW0_001077"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004455 - ketol-acid reductoisomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HFO7473949.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            42308..43201
                     /gene="ilvY"
                     /locus_tag="ACHKW0_001078"
     CDS             42308..43201
                     /gene="ilvY"
                     /locus_tag="ACHKW0_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HFO7473950.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(43253..44797)
                     /gene="ilvA"
                     /locus_tag="ACHKW0_001079"
     CDS             complement(43253..44797)
                     /gene="ilvA"
                     /locus_tag="ACHKW0_001079"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HFO7473951.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(44800..46650)
                     /gene="ilvD"
                     /locus_tag="ACHKW0_001080"
     CDS             complement(44800..46650)
                     /gene="ilvD"
                     /locus_tag="ACHKW0_001080"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HFO7473952.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(46715..47644)
                     /gene="ilvE"
                     /locus_tag="ACHKW0_001081"
     CDS             complement(46715..47644)
                     /gene="ilvE"
                     /locus_tag="ACHKW0_001081"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HFO7473953.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIDVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(47664..47927)
                     /gene="ilvM"
                     /locus_tag="ACHKW0_001082"
     CDS             complement(47664..47927)
                     /gene="ilvM"
                     /locus_tag="ACHKW0_001082"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HFO7473954.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(47924..49570)
                     /gene="ilvG"
                     /locus_tag="ACHKW0_001083"
     CDS             complement(47924..49570)
                     /gene="ilvG"
                     /locus_tag="ACHKW0_001083"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HFO7473955.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQLHC
                     AQLRDEHAWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(49573..49623)
                     /gene="ilvX"
                     /locus_tag="ACHKW0_001084"
     CDS             complement(49573..49623)
                     /gene="ilvX"
                     /locus_tag="ACHKW0_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide IlvX"
                     /protein_id="HFO7473956.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(49710..49808)
                     /gene="ilvL"
                     /locus_tag="ACHKW0_001085"
     CDS             complement(49710..49808)
                     /gene="ilvL"
                     /locus_tag="ACHKW0_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HFO7473957.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            50162..51682
                     /gene="yifB"
                     /locus_tag="ACHKW0_001086"
     CDS             50162..51682
                     /gene="yifB"
                     /locus_tag="ACHKW0_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HFO7473958.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     QAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(51707..52045)
                     /gene="maoP"
                     /locus_tag="ACHKW0_001087"
     CDS             complement(51707..52045)
                     /gene="maoP"
                     /locus_tag="ACHKW0_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HFO7473959.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            52164..53003
                     /gene="hdfR"
                     /locus_tag="ACHKW0_001088"
     CDS             52164..53003
                     /gene="hdfR"
                     /locus_tag="ACHKW0_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HFO7473960.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(53099..53174)
                     /locus_tag="ACHKW0_001089"
     tRNA            complement(53099..53174)
                     /locus_tag="ACHKW0_001089"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(53139..53141),aa:Trp,seq:cca)
BASE COUNT        13139 a        14234 c        13491 g        12316 t
ORIGIN      
        1 tcattaaggc tgtcgagcag gtaagaaacg tccattggca ccgccgcgta aaaataggtt
       61 gggcggagaa gcgcaacgcc ctccgccaaa aagctgggta tatatacaga tttgctgatt
      121 atatcagcga ggtcagagat gccaaccggg aaatttccag atgcggcagt aaacggctgt
      181 cccaggtttg catcagatcg ccattttccg gtctgatcca acaagcctgc attccgctgc
      241 gaattgcccc acccacgtca gtggtgagat cgtccccaac atgtaagatc tcgccgatcg
      301 gcacgttgag tttttccgca gccaaaaagt acatatcgct gaacggtttt gagcgcccgt
      361 gcgggccagc gcgcagcaca aactcaaaat aatcccccaa accaaacagt tccggctggg
      421 cgttaccgtt ggtgatcgcc accagcggcc atttcttcgc cagctgtttt aaggtgtcgt
      481 gagtttgctg cgggacgtcg attcggctgc gccatttggc aaagttaatc attgctgcgt
      541 gtgcgcctgc actggcttct tcggcactca gcccggcgtc gagcatcgct tgttcaatcg
      601 aacgaaaacg ccaacgcgtc acgtcgtgat aaatctcggg ttccgcttcc cgtaccgcct
      661 ggcgcaggcg ttgcagatct tcattctgga agctgcgcag cgccggatga taattttgca
      721 caaaggtaag cgcctctcgc tcggtgcgca aaatcaccgg acggttatcg taaagggtat
      781 catccaggtc aaaggtgagc gccgagatgc gccccaaagg ccggtaaaaa cgcattattt
      841 cccccgtttg gcgcgtggat gcgccgcatc gtacaccgag gcaaggtgtt gaaaatcaag
      901 atgagtatag atttgcgtgg tggagaggtt ggcatgaccc agcagctcct gcacaccacg
      961 aagatcgccg ctcgactcca gcatatgcgt agcgaacgag tgacgtaatt tatgtgggtg
     1021 aacgtgatta ttcagccctt gttttatgcc ccattcggca aagcgtttct gcacattacg
     1081 cgcggagata cgcttgccca gtttcgacag aaaaagcgcg tcgtcttcgc taccaaacag
     1141 gtcgcgcaaa tcaagccagt gctcaatcca cgccacagcg ttgcgaccaa tcggcaggcg
     1201 gcgctctttg ctgcctttcc ccattaccca cacttcacca gactccaggt cgaggtgttt
     1261 gatatccagc cccaccagct cggaaagacg cagacccgcg ccgtacatca cttccagcat
     1321 tgcacggtcg cgtacagcga ggggatcgtt gatatcaata tccagcagcc gattcatatc
     1381 gtcgacgtcg atgtttttcg gcagatgacg cggcgctttc ggtgccgaaa ctcctttcgc
     1441 cgggttagct ttgagttcgt tctggctgac cagccagtca aaaaagctac gtagcgcaga
     1501 aagccgtaac gccagacttg ctgctcccag ccctttacgg cgactgcgta cagcaaaatt
     1561 gcgcaccatc gtcacatcac attgctgcca gctttgcagg ccgttttcgc tggcaaaatt
     1621 gatgatcgcc tcaagctgac gctggtagtt aagcagggtt atcgggctaa gctggcgctc
     1681 cacgctcaga taacgtaggt agcgttctac atcggtgtgt aaatcggtca tacgcgttca
     1741 atccaacgct ccagaagctc cggcaacatc agcgcaattt catgaagtaa ctgcgttcct
     1801 tgcccttgtt gatagtgact ggcatcgcga ctggtaaaca gcacaacacc caaatcagca
     1861 tcgcttccca gcatcgacat cgccaccgat cccaccgctt tcgcttccgg tagcaccacc
     1921 agcaactctg gtccgttaag cggcccaaga tagtgctgtt cctgccccaa acgctgaata
     1981 cgcagcggtt cgaaagactg acggcttaat gccagatgag tgtggttcga cggcgcacct
     2041 aagcgccagc gatccggaaa caggcgcaga ctcgcacctg ccaggccgag atcgcgcgcc
     2101 cagcggtgaa agcgcatcag catatcgtcg agactgctgg cggcggtgag actgcgctgc
     2161 aggtagagta ggcgataaaa caggccttcg ttggcgatag cctgttccat caacagcgcc
     2221 atgttctctt ccagaacatg aatatgatta cgtgcgcggg ccatgtgcca ctcgaccaac
     2281 gaaacggtgc cgcgtaccgg atgcggcaca cgtatcgctt ctactgcgcg cgcattacgg
     2341 ataaaaaact caggattttt aatcagataa tcgacaaccg cccggtcatc aagctccgtg
     2401 agtgtttcct gcagttcttc ccctggttgc ttcatagatg aataaatccg tcgtagacat
     2461 gtaccgccgg gccagtcata tataacgggt gacccggacc tttccaggcg atatcaagac
     2521 gaccgccggg gagttccacg cgtacttctt cggccagcaa accttgctga atccctaccg
     2581 ccaccgccgc acatgcgccg ctgccgcagg cctgggtttc tcctgcccca cgctcataaa
     2641 cgcgtaaacg aatatgctcg cgcttaacca cttgcataaa accgatattg gctcgctccg
     2701 gaaaacgctc gtggctttcc agaacaggac caagcgtttc taccgccgcg gtatcgacat
     2761 catcgacctg aatcacgcaa tgcggatttc ccatcgacac cacgccgcat aagattgtct
     2821 gctcggcggc gcgcataata taggtctttt ccgctttgtt agcgcgaaac ggcacggcgg
     2881 aaggttcgaa gttgggttcg cccatattta cgcggaccag atcatcatcg gtgacggtca
     2941 gaaccatccg cccgttggcg gtgctgacgc ggatatcgcg cttattggtc agccctttca
     3001 gacgcacaaa acgggcaaag cagcgcgcac cattgccgca ctgcgccact tcactgccat
     3061 cagcattgaa aatgcgatag tgaaaatcca gttcaggatc atacggcggc tcaaccacca
     3121 gcagttggtc aaaccctact cccaggtgcc gatcagccag gcgacgaatc agctccggtg
     3181 aaaaaaagac attctgcgtt accgcgtcga cgaccataaa atcgttgcca aggccatgca
     3241 ttttcgagaa ctgcatcatt tactccaatc acgcgggtac agaaactgac ttttagtaat
     3301 tcacctggga tggaccatcg ccagtggcgc gatcgttttt atccggcacc gtggattgcg
     3361 tttgcgtctc taccggtttg gtcggcggcg gtgcgttttt atctgcaggc gggaaataga
     3421 gcggaccttt cagaccgcag cccgtcaggc tgaagagagt aattaataca gtgagtgcct
     3481 taaacacgtt tttcattatg gattgcctgt aaagttcatc gctttctgct ttctatcatc
     3541 gcaggagaag gcgtaaaagc aagcgtttag cgactcttta tgaagtctga aaagtgagga
     3601 aatgctgcga gtataacgct tacacgtggg gatttatgtt ttgctggtag tttcgtgatc
     3661 ggcacctcgc gccaggatgg ctttatgcac tatcgcaatg tggtttgttt gttgtcttgt
     3721 tcattgttct tatcgtcggc atggggcttt taatagagtg gcgcaccatg caatttcgtt
     3781 gtctgacctt agtcgccagt gacatacaac tttgccaacc tggtgataag agggaataaa
     3841 atctgatggg gctatttacc caactggaaa aatttgacca aaagcttacg cgcggttatg
     3901 cccggtgggg gcgctgggta tggcggacgt taattgtcgt tccggtactg gtcgtgttgt
     3961 ggaatatagg acaggcagta tggggaggac caagaggtgg agtgattctg gaaatccaca
     4021 gtgaaattga ccgcccgata ctgggattca gtgttaacgg cgtggcgggc gcgaatgctt
     4081 ttgcaaatgg tggcggttca acaacctgtt gtggtgatgt tagtggggat acggcagagg
     4141 ttatctggac attaagtact actcgtacac agtataatgc tggaatgcgt ctggagaaac
     4201 ggaatatgac gttacctctc cccaaaagag aatggggaga agattttctg catgtgcatt
     4261 ttatgcctgg cgataaagtg ctgctggggt ggagtaaaga ttctttttct ccgtatgaag
     4321 atcttcacaa cggggggtac aagacccgcg ttcggcagga tgtgaaagat aaactttatg
     4381 gtacagggaa aatgaattga tagatttcta taaaaatcac gttcggcaga aagcgtaata
     4441 atgtggggaa tgttagatac cggtggtggg acctaagtac aacacgcgaa agacaataaa
     4501 gtaggtaacg gtgggcagag tcgcatgttg acacccttcc cggcgtcaat ccggcacatt
     4561 ttaacgtgat cagtcacacc atggcgcagt agctcgtatt tatatcgtga atgaaaacct
     4621 caagtttacc ccagcaacta tcagaaaata gcgatggcct ttatagattc tgaaaagtga
     4681 cgatttgccg caaaagttac gcttacgtgc gaggaattat gttctgctgg tagtttcgtg
     4741 atcggcacct cgcgccagga tggctttatg cactatcgca atgtggtttg tttgttgtct
     4801 tgttcattgt ttttatcgtc ggcatgggga tgtcggcttg atccgcctga acataatatt
     4861 taccagaaac agggaaaagg cgtggtatac ctgcgccctt ataaagaaac aaatctttct
     4921 cttcctgacg tgaattacaa aagtttgcgc cgtttaccca atttattaat agacccgaca
     4981 acactagaca aatgggataa agtacctcct ctgactgatc tgacaaccga ctacctctat
     5041 gaaggcgcac aggcctggta tcctcattac tcctggcata gcgatggtcg ctatatcctc
     5101 tacgcgggtg aagttgtgca aaacccgcca gacaaacctc ccgtcgatgt cgcctcgttc
     5161 aaggcgtggg gtgattttgc cgcagataaa cacagtctct atttcgaagg caagcgcacc
     5221 gataacaatg gcgggggaaa tcgcttagat atcaaaacac tgcatcaggt ggaattccgg
     5281 cctccctggg atcctgactt actggggctg atattacgtg acgctaattt cttgtatatc
     5341 aatgggcatc gcctcgcaga tcccgatagt ttccgtgtgc tggcacaaaa gtcatgggat
     5401 cagcgaggaa agttttctac cgcgtttaat ccctgtgtgg ctatgccatt tggcccctgg
     5461 gataccctgg ctcgtacacg gaccaaaatc ctgatcaacg gcgaacagct tgatgccgac
     5521 ccggacacct tttccgtcgt gcgctggatg cccggctcac tcttgacctg gcgtgataaa
     5581 aacgggctgc agcgtaaagt cctcgacaag gaaaatctgg agtgggatga agatttaaca
     5641 aagcactgtc tggatttttc tctgctggaa aagaaagtgt tctggcgtaa aggacctgct
     5701 tgtaaacagg aagaattacc cggactcgat ccggagcagt ttcaacccat cagtgatgct
     5761 gtcgcccagt atcaggactc gctttatacc atcatcgaaa cagagtctgg tgaccgcaag
     5821 ctggaaatcg tgaaacttga tgatcccaat cttattatca acaaacgttt caacgccggg
     5881 aaacgccacg gctatttact tacgcgtgcc gaagggtggc cataccattc cggtttacat
     5941 gtgtttgaat ctgacggacc gctgatctta ctggataacc gttctccgga tgaacgcgaa
     6001 gcccatctta atgaccatcc ctttttgcgc agatggtatg cccgcgataa ccgctacatt
     6061 tacagctttg atggcgcgca gctctggcga taccgcaccg ctgatccgaa acaagttcgc
     6121 ttaatctgga aggaacaaca ttcgggatat ggctatggcg taaattacaa aacgggatat
     6181 ctggacggaa aaattactga tgacggcgaa tttattcctg ccccgcgcaa tgaggcgaca
     6241 aaatgaaggg tcaaataatc acagttgcac tcatcctgtt gggacacaca tttctgacgc
     6301 ctgctgtaca ggccataggg ttcgactatt acaatgatca cggtgttatg tcttacggta
     6361 aaggttattg gggggataag aaaatcattg cccaatttcc gaagatgaac agagccgatt
     6421 tgcgccttgt gacaaatata tccggggagc gggagttggt ggaaggctat atacccaccg
     6481 atgaaataag cataaaaaat gaggaatacc actgggtgac agacggccgc gttattctct
     6541 ggcgtggcaa aatcgttagc aatccaccgg gaacacccac tgtcgatatt gccagctttc
     6601 aggctatggg ccgcttcgcg gtcgataaat atagcctcta ttttgacgga cagcgcaccg
     6661 aaagtaatag cggcgcgtca cgtgttgatc tggcgacact aaaagctatc gaaggtaact
     6721 ctaccacgct ggtggatagt aaaaatctct atttgtccgg tcgacgtcag gggagcagca
     6781 gtaatgttac tgtattagaa aaaagatggt ggggtattaa tccacgtctt atgagtgtaa
     6841 acagaaattt gtattccaat gatctgctta tccgcagtgg gcagaatatt tatttaaatg
     6901 gcgttcacct tacggcgaat gcagactcat ttgagataat tcgctggata cctcattcac
     6961 tgctggtttt tcgcgacaat aagggtatgc atcgttatcc ctttgggcaa ttgtctggca
     7021 aagcgatacc cgtagatgat gacgtctctt ttgaagtagg ggaaagtcgc gttcgctggc
     7081 gtaaacagct cacgtccgac cgtcagtgga gcaagtggat agacctacca gatattgaac
     7141 ctgaacaatt ccatctgatt actggcaata ttgcgcaata taaagatcgg ttgtatgtaa
     7201 caaaattatc gacatttggt gaagaccagc ttgagataat cccgctggat acgccagacc
     7261 tggtcattga tcgctcattt aatagcggca aacagcatgc ttattttatc cgccaattac
     7321 ggtcaaagag cgtgcaaatt attccagtta acggtccgct aactaaaaac gatcgcttcg
     7381 cttatgacga tcgcaatgtt tatacatgga ccgatacaga ggtaaggatt acgccctccc
     7441 cctgcccggc gaaaacacac gtcagagaag agaatgtgcg tgaacttcac aacagagaca
     7501 ttattattcc gttgacggat gactcatgcc ggaacgcagc aacagacggg caaactttga
     7561 agccctgacc ccataccgct atactgccgc catcacttaa agacaggata caacaatgaa
     7621 cgacagtgaa tttcatcgcc tggctgatca actctggctg accattgaag aacgcctgga
     7681 cgactgggat ggcgacagcg atatcgactg cgaaatcaac ggcggcgtac tgaccattac
     7741 ctttgaaaac ggcagcaaaa tcattattaa ccgccaggag ccgctgcacc aggtgtggct
     7801 ggcaaccaaa cagggcggct accattttga cctaaaaggc gatgaatgga tttgcgatcg
     7861 cagcggcgaa accttctggg atttgctgga acaggcggcg acacagcaag cgggtgaaac
     7921 agtcagtttc cgctaagatt gcatgccgga taagcctcgc tttccggcac gttcatcacg
     7981 aaaaatattg ctgtaatagc ggcgtatcgt gatcctgatt ggcaggcggc atgttaccga
     8041 tagattttgt gcggaacgga atcacctgtt cacgaccatc aaccttcaca atctgataga
     8101 actgcggcag gttgaagttg atgaagcttg agccgtaggt gaagcggtcg tgcgatgacg
     8161 agtagaagcg actgacgtca cgcaccagtt cctctttgct gccttcgcag tggtgataca
     8221 cctcaacccg gttgctttcg tcgagaatgt agatattaaa gccattctcg tcttgcgttt
     8281 cttcgaagaa gaactggatg atcccttcac tagcaaagcc gtccaccacc gccggtaatt
     8341 tgacgtgatt ggtttcaacc tgcactgaca agccgtgcag tttgttatgc gaaatagcgc
     8401 cataaaactc gatggcgttt tccagttttt gtaccgatac attcaggcgt tcgaagaaca
     8461 acccccaggt ttgaccagaa acgcgcagcg ccttgaaacg cccggtttcc tggcgggtgc
     8521 tggaaaggcg cagttcaata cactcagaaa ccagttgctg cacgcgagta cggattaacc
     8581 cgcgcagatg ctggctataa cagaagactt ccacgctatc tggcggtgcg gcgtcctgat
     8641 gcattttgcc gagaatagtt ttcagggctt cgatcatcga ttgctcgccg ttgaagtgca
     8701 gcgtacgcac ttcgttccac gagttgcggt acagcaggtc aacgctacct accaggcaat
     8761 tttgattctc gccaaagctg aagacatcca gcttacggaa atcaaaatgc accacctgat
     8821 tgcggaacgc cgctgtcggg tcatattcca ggttaacgat aatcgccaga tggcggatct
     8881 cacacgggct gtagagcgcc ttcggtgtcg gtgcaggtaa gcgcagcggg aaatggtgcg
     8941 acacgtcggc gaccatctcc tgcaacttag gcaaatcgac aatgccgtta cctttaatat
     9001 acaaacgggt gcgcgaggtc agcaggccgt taaaccatgc ccacgccacc agtttgttca
     9061 ggtaacggtt atattccagc ggctgatggc tgatgatcga ctcaatattt ggcgcgcggt
     9121 tatacagata ccaacctgaa cggttagcac ggcccggcgg cacataaata aaggtcagat
     9181 tcggttccga gagatcgggt gaaatctgcg ggtttaccag cgtcacttta cctggtaatg
     9241 cttcaaacgc ggcatacagc ttacgcgtca gcacgccgat atcctgcgga ctggcggaga
     9301 cgctaaggtt attgcgacgc gcaaagcgga tcagattacg gtagctctgc atcatcgcgt
     9361 cgagcaactc gttgtgcgcc tcacgcacct gatcaatctt ccagttagcg cggttatcga
     9421 gcattgccag acgagcttcg tcccaacccc actcgctcac taactggctc aacactgcgc
     9481 gacgccagcc tacgcaggcg cgttcacggc tgagcttttc gcacactttt aaatagaagc
     9541 agcgacgtac taaatccaga cgggtaaaat cttcaatcgc cgtcaggtat tcagtaacac
     9601 gctccagcat catgcagtat ggatcgagac caaacgatac aatctcgccg tcgtgcaaac
     9661 gctgtttgat atctttcgcc agcagacgtg ggttcgggta ttcccaggaa taggcttcca
     9721 gcagcagtgt tttcagtacc gctttgtatg gggaatcgat actcttgtag agctgccaaa
     9781 ggctggcacc aaagtactct tcagcagaaa gcgagcttaa gccaccgaga tccagccatt
     9841 catttggcgt cagcacgccc tgcgcgtaaa gcgtcatcac atagtcgtcg taatgctctt
     9901 cttcgtcgca cggcaccata ttccacagaa tacgcttacc ggcgagacgc acggcggtac
     9961 gataaaattc gtcaagcagc agtatatgct gggtggagcc acaatcttcg ccccccaggc
    10021 tgccgctttc attatgacgg aagcggtttt catcaatcag gaagaagctg acttccacac
    10081 ccagcgaggc ggcccagctt tccagcagac tacatttacg ttgtagtaac tggcgctctt
    10141 cgctatcgag ccaggattga tgacagaccc agatatccag gtcagaggaa caactttgcc
    10201 ctacggacga ggtgctgccc atggtgtata caccagtaat tggaagctca cctttcggtg
    10261 ggtcctgtac tgacattcca cgatacagtt caagctcgtt caggtagtgg cgttgagttt
    10321 catcaggcgt gtaaaggcaa atgcctttgg gaacgttacc atcaaggtaa cccggcatta
    10381 gcggatggtg atagtgcaac aatgtcggca gtagactgta gacctgttgg aatgcaggcc
    10441 ccatagcagc aagcgcgcga tccacacgca attgatttat ggcatccagt ctctgtttca
    10501 gagtctcaat atagaggtac aagacgtatc gcctgatttg ctacccgtca tgactgtgat
    10561 tccgccaaca tcaacggtaa cacgcggcat tcgggatatt tcgtatgtca aaggtaaccg
    10621 ttaccacttt tcgcgcctgg tttttttagt ttcacgacga aaaaatggtc taaaacgtga
    10681 tcaatttaac accttgctga ttgaccgtaa agaaagatgc gctacataca agtgtagcac
    10741 cgtttattct ctgtaaattc cttattacga cggcgtgaaa cgcctgtcag gatccactgc
    10801 cagacctcat tttacggttt gcgcaggcgt ctacgtttca ccacaacact gacatcactc
    10861 tggcaaggat gttaggatgg accacggatg ataatgacgg taacaagcat gttagacaat
    10921 gttttaagaa ttgccacacg ccaaagccca cttgcactct ggcaggcaca ctatgtcaaa
    10981 gacaagttga tggcgagcca tccgggcctg gtggttgaac tggtaccgat ggtgacgcgc
    11041 ggcgatgtga ttcttgatac gccgctggcg aaagtaggcg gaaaaggctt atttgtaaaa
    11101 gagctggaag tcgcgctcct ggagaatcgc gccgatatcg ccgtacattc aatgaaagat
    11161 gtgccggttg aattcccgca aggtctgggg ttggtcacta tttgtgagcg tgaagatcct
    11221 cgcgatgcct ttgtgtccaa taattatgac agtctggatg cgttaccggc aggcagtatc
    11281 gtcgggacgt ccagtttacg tcgccagtgc caactggctg aacgccgccc ggatctgatt
    11341 atccgctccc tgcgcggcaa cgtcggcact cgcctgagca aactagataa cggcgaatac
    11401 gatgccatca ttcttgccgt agccggacta aaacgtttag gtctggagtc ccgcattcgc
    11461 gccgcgttac cacccgagat ttctcttccg gcggtaggac aaggtgcagt cggtattgaa
    11521 tgccgccttg atgatacgcg cactcgcgag ctgcttgccg cgctgaatca ccacgaaact
    11581 gcactgcgcg ttaccgcaga acgcgccatg aatacccgtc tcgaaggtgg atgtcaggtg
    11641 ccaattggta gctacgccga gcttattgat ggtgaaatct ggctgcgtgc gctggtcggc
    11701 gcgccggacg gttcgcagat tattcgcggt gaacgccgcg gtgcgccgca aaatgccgaa
    11761 caaatgggga tttcgctggc agaagagcta ctgaataacg gcgcgcgcga gatcctcgct
    11821 gaagtctata acggagacgc cccggcatga gtatcctggt cacccgcccg tctcccgctg
    11881 gagaagagtt agtgagccgt ctgcgcacac tggggcaggt ggcctggcat tttccgctga
    11941 ttgagttttc tccgggtcga caattaccgc aacttgctga tcaactggcg gcgctggggg
    12001 agagcgatct gttgtttgcc ctctcgcaac acgcggttgc ttttgcccaa tcacagctgc
    12061 atcagcaaga tcgtaaatgg ccccgactac ctgattattt cgccattgga cgcaccaccg
    12121 cactggcgct acataccgta agcggacaga agattctcta cccgcaggat cgggaaatca
    12181 gcgaagtctt gctacaatta cctgaattac aaaatattgc gggcaaacgt gcgctgatat
    12241 tacgtggcaa tggcggtcgt gagctaattg gggataccct gacagcgcgc ggtgctgagg
    12301 tcactttttg tgaatgttat caacgatgcg caatccatta cgatggtgca gaagaagcga
    12361 tgcgctggca atcccgcgag gtgacgatgg tcgttgttac cagcggtgaa atgttgcagc
    12421 aactctggtc gctgatccca caatggtatc gtgagcactg gttactacac tgtcgactat
    12481 tggtcgtcag tgagcgtttg gcgaaactcg cccgggaact gggctggcaa gacattaagg
    12541 tcgccgataa cgctgacaac gatgcgcttt tacgggcatt acaataactc tcataacagg
    12601 aagccataat gacggaacaa gaaaaaacct ccgccgtggt tgaagagacc agggaggccg
    12661 tggacaccac gtcacaacct gtcgcaacag aaaaaaagag taagaacaat actgcattga
    12721 ttctcagcgc ggtggctatc gctattgctc tggcggcggg tgtcggtttg tatggctggg
    12781 gtaaacaaca ggccgtcaat cagaccgcca ccagcgatgc cctggctaac caactgacgg
    12841 cattgcaaaa agcccaggag agccaaaaag ccgagctgga aggcattatt aagcaacaag
    12901 ctgcacagct taagcaggcg aatcgtcagc aagaaacgct ggcaaaacag ttggatgaag
    12961 tccaacaaaa ggtcgccacc atttccggca gcgatgctaa aacctggctg ctggctcagg
    13021 ccgattttct ggtgaaactc gccggacgga agctgtggag cgatcaggac gtcacgaccg
    13081 ctgcagcgtt gctgaaaagt gcagatgcca gcctggcgga tatgaatgac ccgagtctga
    13141 ttaccgttcg tcgggcaatt accgatgata tcgccagcct ttctgcagtt tcgcaggtgg
    13201 attatgacgg catcatcctt aagcttaatc agctttcaaa tcaggtagat aacctgcgtc
    13261 tggccgataa tgacagcgat ggttcgccga tggattccga cggtgaagag ctttccagtt
    13321 ccatcagcga atggcgtatc aatctgcaaa aaagctggca gaactttatg gacaacttca
    13381 ttgcgattcg ccgtcgtgat gacaccgccg taccgctgtt agcgccaaat caggatatct
    13441 atctgcgcga aaatattcgc tctcgcctgc tggtcgcagc acaagctgta ccgcgtcacc
    13501 aggaagagac ttatcgccag gcgctggaga acgtctccac ctgggtacga gcttactacg
    13561 atactgatga tgccaccacc aaagcgttcc tcgacgaggt ggaccagtta agccagcaaa
    13621 atatctcgat ggatcttccg gaaaccctgc aaagccaggc gatgctggaa aaactgatgc
    13681 agacccgcgt gcgtaacctg ctggcacaac cggcagcggg gacaacggaa gctaaacctg
    13741 cacctgcacc tgcacctgca cctgcacctg caccgcaagg tgatactccg gcagccgcgc
    13801 cgcaaggaga ataaccatgc taaaagtgtt attactcttt gttttgctga ttgcggggat
    13861 agtggttggc ccgatgattg ccggtcatca gggttacgtg ctgatccaga ctgacaacta
    13921 caatatcgaa accagcgtca cgggcctggc gattattttg attctggcga tggtagtgct
    13981 gtttgccatt gagtggctac tgcggcggat cttccgcaca ggcgcacaca cccgtgggtg
    14041 gtttgtcgga cgtaagcgtc gccgtgcccg taagcagacc gaacaggcgc tgctgaaact
    14101 ggcggaaggc gattatcagc aagttgaaaa gctgatggcg aaaaatgccg atcacgcgga
    14161 acaaccggtg gtgaactatc tactggctgc cgaagccgcg caacaacgtg gtgatgaagc
    14221 acgcgccaac caacatctgg aacgcgcagc agagctggcc ggcaatgaca ccattccggt
    14281 agaaatcacc cgtgtacgtc tgcaactggc ccgtaatgaa aaccatgctg cacgccacgg
    14341 cgtggataag ctgctggaag ttacgccacg ccatccggaa gtattacgtc tggcggaaca
    14401 ggcgtatatc cgcacaggtg catggagttc tctgctggat atcatcccat caatggcgaa
    14461 agcccatgtt ggtgatgaag aacatcgtgc aatgctggaa caacaggcat ggattggcct
    14521 gatggatcag gcgcgtgccg ataacggtag tgaaggtttg cgtaactggt ggaaaaacca
    14581 aagccggaaa acgcgtcatc aggtagcgtt gcaggtggca atggcggaac atcttattga
    14641 atgtgacgat catgatactg cccagcaaat tatcatcgat ggcctgaaac gccagtacga
    14701 cgatcgccta ctgctgccga ttcctcgact gaaaaccaac aatccggaac agctggaaaa
    14761 agtgctgcgc cagcagatca aaaacgtcgg cgatcgcccg ctgttgtgga gcacactggg
    14821 ccagtcactg atgaagcacg gagaatggca ggaagcatcg ctcgccttcc gcgcggcgct
    14881 gaaacaacgt ccggacgcct acgattacgc atggcttgcc gacgcgctgg acagactgca
    14941 caagccggaa gaagctgcag cgatgcgtcg cgacggtttg atgttaacgt tgcagaacaa
    15001 cccaccacag tagttccttc tcacccggag gcaagcacct ccgggacctt tctgatgcat
    15061 aaaaaaacgc ctgctcttat tacggagcag gcgttaaaac aggtctgtat gacaacaagt
    15121 gggtgcttca ctcaacgttg tgtccatggt gtctgatgag gcataagcga catctgtcag
    15181 tggacgataa gcaccgtaaa cggctctgcg tcattccgga gtttatgagg cactaaggcg
    15241 aacataagag atggaatgag catctacccg tttattatgc cacagagaat cgggaaataa
    15301 catcccttaa cacttgttat gagataattc tgtaatcctc tttgcttcct gagtaataac
    15361 ttcctgagtg aatatttaac ctgagcttga tcctacacat acttattatg aatgataaaa
    15421 ttcattcaat taataacaca tatattaatt gccgttaaaa ctaaaaaagc atcaataatc
    15481 aacgtgatat aataaacctg ccttacatat caactgcgcc agaggtagga ttgaaaacgc
    15541 tctcctgatt ttccaattca ttttctggat aaataaataa tttattttta tcactattat
    15601 ttatgtaatc atcctgtcag ggagagggat ctcaattatc aatgcttaat tacgtcatca
    15661 ttttgatgac atgaaaccgg agcattttgc atggaatttt cgttttcacc caaacttctt
    15721 gttgttgctg tcgccgccgc tcttcctctc atggccagcg cagcagatac cccgtcaact
    15781 gccaccgcac gcaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
    15841 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
    15901 aatcaaccgc aggcggccac aaaatggccg cttttttact tattcaccac cagcaaatgt
    15961 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
    16021 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
    16081 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccagca cgcttttaac
    16141 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
    16201 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
    16261 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
    16321 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
    16381 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcgaat
    16441 atcgccacca gaaattcacc aaattcatcg gggcgcaccg accattccgt cagttccccc
    16501 tgaatatcac caggcgcatg tagtttcagt ccatcgctgg cagctgtttc atcagccagg
    16561 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
    16621 acctgcaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
    16681 tgatgttttt gcagcagcgt cagggcacgc atcaccagct tatgcgttgg tctgccacct
    16741 ttggtcacgc gatattgatt gtggatctca gccggaccgt ccagcgataa cccaacaaga
    16801 aaatgatttt ctgccagaaa tgcacaccat ttatcatcga gcagcacgcc gttagtctgg
    16861 aagctgttac ttatcttcct gccagcaccg tatttcgcct gtaactttac ggcacggcgg
    16921 taaaaatcca gcccgagtaa cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
    16981 tgcggttcgc tggcagcgat atagtgacgg acatacgctt ccagcgtgtc atcgtccata
    17041 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
    17101 ttcagattac aatcggaacc actcggtttc gccatcacat gaaaagcacg cgttggaacc
    17161 tgttgcagca tggtcactcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
    17221 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
    17281 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
    17341 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
    17401 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
    17461 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
    17521 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
    17581 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
    17641 gtattactac caccgcagct caagcgccgg gactaaatgg cgcgcccgac aggattcgaa
    17701 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
    17761 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
    17821 attgcgcgtt tggttacggt ttgtgcgttt tgctgattat tcctccagtt tatgcgcttt
    17881 cccgtggcga ttaaggccaa aaactttata aatcgccgtc accgccagca taaagatgat
    17941 gccaacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
    18001 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
    18061 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
    18121 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
    18181 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
    18241 cgcaacgggt acgccgtgac gggaaacttt cgccatcgcc gccggtaact gacggttttt
    18301 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
    18361 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
    18421 agtcagtacg aacgggctgc cgttgctgcc tatttcattc cacgggaaga tggtgacgat
    18481 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcaccttgc ctacggcact
    18541 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
    18601 ctggtaggac gccaccacaa tacacagcgc ggtcaggaac cctttccagc cacccgcaaa
    18661 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
    18721 aaagaaaatc acgcccagac caatgacaat catcacgata atcgtggtga ctttgatcat
    18781 cgcgaaccag aactcgattt cgccgtacaa ccgcaccgcc gccagattcg ccaacgccac
    18841 cagcgccact gcgatcaatg cgggtatcca ctgcgccatc tccgggaacc agaactggac
    18901 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accaataaga
    18961 ccaggcggtg agataaccaa agaacgggct catataacga tgcgcataaa cggcgaacga
    19021 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaagacgaa
    19081 tagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca aggtactggc
    19141 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
    19201 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
    19261 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
    19321 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
    19381 aagcagacag gcgacggagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
    19441 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
    19501 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
    19561 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
    19621 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
    19681 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
    19741 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
    19801 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
    19861 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
    19921 gcgggtactt tttacgtact gaacgtacaa cgctgatgcc atccgcatat ttaaattcgg
    19981 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
    20041 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcgt
    20101 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
    20161 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
    20221 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
    20281 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
    20341 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
    20401 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
    20461 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
    20521 cgtaggcgag atcgccagtc cggagtggtt attcagcact tcccaggtaa agtagttggc
    20581 tgagttcagc accatactcg ggcgacccgg ccacagccag gaagggataa agacatagaa
    20641 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
    20701 cgccagattc tcccatggcg agaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
    20761 ttcatcgccg ctcacattca ttccatagcg ttttagcgcc agccagaaca tgcccacaat
    20821 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
    20881 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
    20941 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctatcctg
    21001 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
    21061 cacgccggag acttcactgg agaagatctg actgtaggag ttaagccgga acagcaaaaa
    21121 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atgcccatca ggatcaccca
    21181 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
    21241 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
    21301 aagcaacgcc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
    21361 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
    21421 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
    21481 gcagagcagc cagacaacaa acaggccact gaattgcagc agactcatgc gacctccccg
    21541 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
    21601 gtgtttttat ccaccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
    21661 tcgtcggtag taaacagcac cggcaaatgc tgttccgtca tatcctgcca gaacggattt
    21721 tcccggttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
    21781 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
    21841 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
    21901 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
    21961 acttttaccg tatcgccaaa ttgctgatga acggcgcaca aggcagcaat atggtcattg
    22021 ctgcggtcac cggagttccc caccagaatg gtcattttcc cttcacgttg ccgatcgttc
    22081 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc gccccgcacc
    22141 tttggatgcg ttttggcaaa aaagctcaaa tcgccgcggg cggcaaatac acagccgact
    22201 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
    22261 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
    22321 agcagagcca gccacagtgt gggattgaac tgaccgtgga agaaaaaacg ctgctgacgg
    22381 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
    22441 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
    22501 cgcgcgtgct cgctcgtcgc agccagcgcg tcattgaaaa accgcaaaac ggttcggtta
    22561 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
    22621 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
    22681 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
    22741 actggctgat ttccgccaga atataaaacc gcaatgacgc tttggcgatc accagataac
    22801 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
    22861 gcatagcggt aaatttattc gacaacagca gccagatagc aaaatcacgc agcagccaga
    22921 cggtaaaact cgccgccgcc actgccggta agacgaattt cagcgattta accacttccc
    22981 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
    23041 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
    23101 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
    23161 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
    23221 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttgatc aacatgatag
    23281 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
    23341 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
    23401 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
    23461 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
    23521 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
    23581 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
    23641 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagctccg gcaagcacgc
    23701 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
    23761 acacagccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
    23821 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
    23881 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
    23941 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagcg ttcccccgca
    24001 gggcaaccgt gcagcggaat gtaatggaac accgccatga tttccgcttc tttcagaaag
    24061 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
    24121 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
    24181 aacgcatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
    24241 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
    24301 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
    24361 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
    24421 tcaccacccg ccgtatagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
    24481 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttctaccaca
    24541 aacagattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
    24601 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ccgcttcaat cagcgtttcg
    24661 tcgatgttca tggtgtccgg gcgaacatcc acaaaaacga tttttgcgcc acgcagcaca
    24721 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
    24781 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
    24841 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
    24901 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
    24961 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
    25021 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
    25081 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
    25141 acctacaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
    25201 agagacataa ccacgaatac cgccggacgc cgcacgtaaa atcagacatt gatgatcaaa
    25261 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgtctgctgg cgtcaggcgc
    25321 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctggctaact gacgtaatgc
    25381 gggaatatcg gtctcttgtg ccactacagc accactatca ctgacattgt tcacgggtag
    25441 tgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
    25501 acccgtattt gatgccgcaa ttttggcctg cacccgtgac cacggcgcta acacgtctgg
    25561 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
    25621 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
    25681 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
    25741 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    25801 ctactgtctg cacaaaggta ctggcttcaa tcaggctgtc gtgagtgcca gtgtccagcc
    25861 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    25921 tgatggaggt aatctccagt tcaccacgct ccgacggctt cacctgcttt gcgtactcca
    25981 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    26041 gcttttcttc cagcgagata gcgcggaaat tgtcgtcaaa ctccaccacg ccaaagcgtt
    26101 ccgggtccat cacctgatag ccaaataccg tcgccccttc cgtgcgcgcc gcaacatgac
    26161 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    26221 gttcaccgtt gaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    26281 gctgttcggc gtattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    26341 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccaacatca
    26401 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    26461 cgccgcgcgt aatcggatgc aatcgggtgc cggaaccgcc cgccaggata atacctttca
    26521 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcgccctga tagctgccgt
    26581 cctgcacctg cttccaccag ctttcattag ccagatacca ctgcaccgtt ttacgcattc
    26641 cactttcaaa ggtttcctgt ggcagccaac caagttcacg ggcaattttc gaagcatcaa
    26701 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcaag tcacgatagt
    26761 gcaccacgcc gtgcggcttg ttcggagcca gttcttccag cagctcgcaa atggtttcca
    26821 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    26881 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    26941 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    27001 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    27061 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    27121 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    27181 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    27241 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    27301 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    27361 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    27421 gatcgcagat atcaactttc tcaaaggcaa agtgctcgct ttgcgcgacc ggtgccagcg
    27481 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    27541 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    27601 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    27661 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    27721 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    27781 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    27841 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    27901 gattttcagt tcactggcgc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    27961 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    28021 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    28081 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    28141 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    28201 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    28261 agtgacgaca cactcacctt cgaggaaaat tttgtacagt tcgctggcgc gggtcgaaca
    28321 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    28381 cggtaacacg cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    28441 tgggaaagtg agatccggac gcatctctgc taaccattct gccatcttct cggttgaccc
    28501 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    28561 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtggg
    28621 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    28681 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    28741 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    28801 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    28861 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    28921 ccatcaccat acgggttatg ggcgcggctc atagtttgat attcgttttc gtcttttaaa
    28981 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    29041 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    29101 gaaggcgctt cttcctgaat gccgcctgag tcggtcaaaa tcagccaggc gtggttcatc
    29161 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    29221 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    29281 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    29341 ccgaaacttt cacgcctgtg accggtcacc agaatcatct ttttatcggg gtcgataaac
    29401 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg atcacgcacc
    29461 cataacagtg catcaatgac tgtattacca gtaatgaaga ttcggctatc cgcaacgttt
    29521 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    29581 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagatcgcc cgtgcgtaaa
    29641 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    29701 gtcgttgttg tgtcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    29761 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    29821 ttgagatcgt agtcaggtac aatggaaaag agtttcagca cctgatcgag catctcccga
    29881 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    29941 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    30001 attctcttcg aataagcggc gagcgccttt gcgctcgcag cagcagtgtt gctatttcga
    30061 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    30121 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    30181 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    30241 ccgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    30301 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    30361 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    30421 gctgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    30481 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    30541 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    30601 ttcggcaata agcttcacgc tgtcattgac cgcgcgggta aagtctccgg ggataaactg
    30661 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    30721 ctgcttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    30781 cataacaaac tctttatagg cttcgtccat gaccgatggt tggtcggcag aagccatgtt
    30841 tgaacgggca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    30901 caccgttgga cgatcggtaa tcgccgtcgc gctccactct tgtcgagcaa aaaaagtata
    30961 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    31021 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    31081 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    31141 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    31201 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    31261 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    31321 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    31381 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    31441 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    31501 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    31561 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    31621 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    31681 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    31741 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    31801 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    31861 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    31921 tttacgctga tatcaaaacg gtcatccagc gcgccaatga aaacaagcac accggcacaa
    31981 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    32041 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    32101 tttggtttat ccactaaacc gacttttttt gccactttac gggcaaaaaa cagaaacagt
    32161 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    32221 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    32281 gtccgttccg aacccaccag tcctgtgcaa aaattaacca tttgatatgc aaattgcata
    32341 acatgccagc aaattccagg aattttccta acatcgccag cgcggcatta agattacaga
    32401 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    32461 gtttcatcat ttcgaagaaa tcgtcattgg tcttagtcat ggccagttta ttaatgagga
    32521 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    32581 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    32641 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    32701 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    32761 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcaccg aagaagcgtt
    32821 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    32881 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    32941 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    33001 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    33061 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    33121 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    33181 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcgg
    33241 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    33301 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    33361 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    33421 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    33481 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    33541 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    33601 tggcaaaaat aatgtcctgc ttacgcatac gagccaggtt ttccagcccc atattttcgc
    33661 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    33721 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    33781 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    33841 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    33901 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    33961 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    34021 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    34081 agacagtgca cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    34141 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    34201 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    34261 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    34321 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    34381 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    34441 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    34501 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    34561 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    34621 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    34681 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    34741 ggtgaatcaa ccacgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    34801 tgccgacgca gaaccgctgg cggaagctac cggcctgaag ctgggtctgg cttacggtgg
    34861 tgatggctac gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    34921 tacggggcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    34981 ggtggtactg gacgaagccg atcgcatgta cgatctgggc tttattaaag atatccgctg
    35041 gctgttccgc cgtatgccgc ctgcaaacca gcgcctcaac atgctgttct ccgccacgct
    35101 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    35161 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    35221 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    35281 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    35341 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    35401 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    35461 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    35521 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    35581 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    35641 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    35701 cccacgtaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    35761 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    35821 cccgatgagc atactgtagt aagtgattca ggtgaaagaa tgcagccaac acagagacag
    35881 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    35941 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    36001 gaataaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgcc ctgtctaatg
    36061 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    36121 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg tcttgccgtc aatgcgggtg
    36181 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    36241 aggaagcacg tctgatttat cagggcgttg ctcacacaac tggcggtgcc gatcagcgcc
    36301 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    36361 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    36421 gtaatctggg gcaggaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    36481 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    36541 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    36601 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    36661 acgggctgac gctggaacgt gcgttagtgt tcccgagtgg tctggcgatc ctgatcgcca
    36721 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    36781 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acgctgcgta
    36841 atattcagcg ccgctttatg atcgatattg atcaggcgca gcgcgtagcc aaagttgcgg
    36901 ctaacttctt cgatcaggtg gaaaaggaat ggcatcttga agcaataagc cgcgatttgc
    36961 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgactttaaa caagcgccgc
    37021 aacacgctgc ttatctggtg cgtaatctgg atcttcccgg ttttaccccc gcacagaaaa
    37081 aattgctggc gacgctactg ctcaaccaga ctaatccggt cgatctctca tcgctgcatc
    37141 agcaaaatgc cgtaccgccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcca
    37201 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    37261 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    37321 agattattgc tcaggaaagc cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    37381 aatgagtaag tgccggatgc gatgctgacg catcttttcc ggccttgatt atttccctcg
    37441 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    37501 ttcggcgctg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    37561 caaaaagcgg ctcggctccg ggcgcaccag ctcgccgtac tgacggcgtt ctttacacag
    37621 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    37681 atcgatatta tcttcatcga tgctgctctg gtgcggcaaa aacccttctt ccataccgac
    37741 catgtagaca taaggaaact ccagcccttt tgacgcgtgc agagtcatca gttgcacctg
    37801 atccagctct tcttcactct caccacgctc catcatgtcg cgcaaagtaa agcgcgtcac
    37861 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    37921 gctaaacagt tggttgacgt tcttcatgcg catttcggcg gctttcgggc tgggcgatgt
    37981 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    38041 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtaaagcggg tcaatgcttc
    38101 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    38161 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctct
    38221 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    38281 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    38341 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac actcgcgact gatggttacc
    38401 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    38461 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa ctttaagctc
    38521 tgtgccgtaa cccagttcgg agaacagacg cttctcaaag acgtgcgggt tattggcgat
    38581 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    38641 caccgtcagc gccggaaaat cctgactcag cagcaccagg ttttgtggac gtgcgccgcg
    38701 ccaggagtag atcgactggt cgtcatcgcc caccacggta aagcgcgcgc ggctgcccac
    38761 cagcagtttc accagctcat actggctggt gtttgtgtcc tgatactcat ccaccagcag
    38821 atagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gcagcaacgt
    38881 cggcaataaa atcagatcat cgaagtcgag aacattgcac gctttcaggt gtgcatcata
    38941 cagcccataa caatgggcaa aaatacgatc ccgctcgccc tttgcctctg ccgccgcctg
    39001 cgccggagtt ttgagatcgt tcttccagtt ggagatggtc gaaatcagct gttgcaggag
    39061 aactttgtca tcttcaatca gcccctcggt caactcttta agcaaagcaa gctgatcggt
    39121 atcgtcaaac agcgaaaagt tcgccttcat cccaagcgcc gcatactcgc gtttgatgat
    39181 atccaacccc agcgtgtgaa aagtggagat catcagcccg cgcgcctctt tgcgacccag
    39241 cgtctgcccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    39301 gatatgtcgc gcctgataac cgcaaccgcg gatcagatgg gcgattttat tggtgatcac
    39361 acgagtttta ccggaacccg cgcccgccag caccaggcag gggccggtaa cgaattcgac
    39421 agcttgttgt tggccggggt ttagacgcat aggtgtattg ctcaatcttc gaacgggggg
    39481 aggattgtag cagaatcgca tttgaatgtg accgcgtgta agccgctaac cggttggctg
    39541 cggatgttta aatcagagag agaagaccga aatcggagtt catcgtgaat ccattagact
    39601 tagaaaatat cgggtcgtaa cgttgtgtta ttactttttc agagtttact aaggagaggt
    39661 ttactcgtgg cgattaattt cagccctaag gttggtgaaa tacaggaatg caactttggt
    39721 aactatcctg ttatccagaa tggacagttc agcaccacgt attacgatgg ccgcattcct
    39781 cctgaaatga taaaaaacag gttagttgtc gtgctcaacg gaaaaattaa tggcaacgca
    39841 tgtatcgtcg ttccattatc aaccactcgc gatcatgaca agttaaatcg tggaatgcat
    39901 gttgaaatag ccagtaatgt tatcaatgtc ctgcaattct ttgatcagca gattcgttgg
    39961 gccaaagccg acctggtaca acaagtcagt cgaaaccgat taaatagagc aaggacttac
    40021 agagggtatc ttaatcaatg tttgccacat gagttggttg cagacataca gagggcggtc
    40081 atcaagtcaa tcaatgccat atcgctcata aattgacaat aaaataacca tccccgataa
    40141 tgactttgta gcccatagag gccctaagtt ttctcccctt gagggacctg cgaagcccgg
    40201 attttccggg cttttgccat atatgtacca gcccctccct gactgacaat tacgttagca
    40261 tctgcggatc atgctaaaat cgccgcctga caattattca ctcaaggcac gatcatggca
    40321 aaaacagcag cagcactgca tatccttgta aaagaagaga aactggctct ggatcttctc
    40381 gagcagatta agaacggtgc cgatttcggc aagctggcga agaaacactc catttgccca
    40441 tcaggcaaac gcggcggtga tttaggtgaa ttccgccagg gtcagatggt tccggcgttc
    40501 gataaagttg tgttctcttg tccggtactg gagccgaccg gcccgctgca cacccagttc
    40561 ggttaccaca tcattaaggt gctgtaccgc aactaatagc aaggccttct ccaggagaag
    40621 gccttgagtg ttttctccct ctccctgtgg gagagggtcg gggtgagggc atcagcgcgc
    40681 acttaacccg caacagcaat acgtttcata tctgtcatat agccgcgcag tttcttacct
    40741 acctgctcaa tcgcatggct gcgaatcgct tcgttcacat cacgcagttg cgcgttatct
    40801 accgcgcctt ccggaatagc tttacccaag tcgcccggtt gcagctctgc cataaacggt
    40861 ttcagcaacg gcacacaagc gtaagagaac agatagttac cgtactcagc ggtatcagag
    40921 ataaccacgt tcatttcgta cagacgctta cgggcgatgg tgttggcaat cagcggcagc
    40981 tcgtgcagtg attcataata tgcagactct tcaatgatgc cggaatcgac catggtttcg
    41041 aacgccagtt caacgcccgc tttcaccatc gcgatcatca gtacgccttt atcgaagtac
    41101 tcctgctcgc cgattttgcc ttcatactgc ggcgcggttt cgaaggcggt tttgccggtc
    41161 tcttcacgcc aggtcagcag tttcttatca tcgttggccc agtccgccat cataccggag
    41221 gagaactcgc cggagatgat gtcgtccata tgtttctgga acaggggtgc catgatctct
    41281 ttcagctgtt cagacagcgc gtaagcacgc agtttcgccg ggttagagag acggtccatc
    41341 atcagggtga tgccgccctg cttcagcgct tcggtgatgg tttcccaacc gaactgaatt
    41401 agtttttctg cgtatgccgg atcggtgccc tcttccacca gcttgtcgaa gcacagcagc
    41461 gagccagcct gcaacatacc gcacaggatg gtttgctcac ccatcaggtc agatttcact
    41521 tccgcaacga aggacgattc cagcacgccc gcacggtgac caccggttgc tgccgcccat
    41581 gctttagcga tcgccatacc ttcgcctttc ggatcgtttt ccgggtgaac ggcaatcagc
    41641 gtcggtacgc cgaatccacg tttgtactct tcacgcactt cggtgccagg gcatttcggc
    41701 gcaaccatga ctacggtgat atctttacgg atctgctcgc ccacttcgac gatgttgaaa
    41761 ccgtgcgagt aacccagcgc cgcgccgtct ttcatcagtg gctgtacggt gcgcactaca
    41821 tcagagtgct gcttgtccgg cgtcaggtta accaccagat ccgcctgcgg gatcagttct
    41881 tcgtaagtac caactttaaa accgttttcg gtcgctttac gccaggacgc gcgcttctcg
    41941 gcaatcgctt ctttacgcag agcgtaggag atatcgagac cagaatcacg catgttcagg
    42001 ccctggttca gcccctgtgc gccacagccg acgataacca cttttttacc ctgaaggtag
    42061 ctcgcgccgt cggcgaattc gtcgcgcccc ataaagcgac atttgcccag ctgtgccagc
    42121 tgctggcgca ggttcagtgt attgaagtag ttagccatgg tgattcctcg tgatgttgtg
    42181 cttcttattg ttcggttcgc gtttgcgaga ttgaattcac tatatgacag gaaatttatt
    42241 gcggaaattg atatattcac aacgtcacat tgcaatttct gcaacgtcaa cattgagggc
    42301 tgcgccattg gatttacgcg atctgaaaac cttcctgcat ctggcggaaa gccgccattt
    42361 tggccgcagc gcgcgggcga tgcacgttag cccatccacg ctctcacggc agattcagcg
    42421 cctggaagaa gatcttggtc agccgctatt tgtgcgcgat aaccgcacgg taacgctgac
    42481 cgaagcgggc gaagagttac gcgttttcgc ccagcaaaca ttgttgcagt atcagcaact
    42541 gcgccacacc atcgatcagc aagggccgtc gctctctggc gagttacata ttttctgctc
    42601 ggtgaccgcc gcttacagcc atctaccgcc gattctcgac cgcttccgcg cggaacaccc
    42661 gtcggtggag atcaaactca ccactggtga tgcggcagat gcaatggaaa aagtggtcac
    42721 gggagaagcg gatctggcga ttgccgggaa accggagacg ctacccggcg ctgtggcgtt
    42781 ttcgatgctg gagaatctgg cagtagtgct gattgccccc gcgctgccct gcccggtgcg
    42841 taatcaggta tctgtcgaaa agcccgactg gtcaacggtg ccgtttatta tggccgatca
    42901 ggggccggta cgccgccgca ttgaactgtg gtttcgacgc aataaaatca gcaacccgat
    42961 gatttacgcc acggttggcg ggcatgaagc gatggtatcg atggtggcgc tcggctgtgg
    43021 tgtggcgctg ttgccggaag tggtgctgga aaacagcccg gagcctgtac gcaaccgggt
    43081 aatgatttta gatcgcagcg atgagaaaac gccgtttgaa ctgggcgtgt gtgcacaaaa
    43141 aaagcggctg catgagccgc tgattgaggc gttctggagg attttgccga accacaaatg
    43201 acgttgtcgc gcgggtaggc ctgataagcg aagcgctatc aggcattttt ccctaacccg
    43261 ccaaaaagaa cctgaacgcc ggattattgg tttcgtcgtg gcaatcgtag cccagctcat
    43321 ttagccgggt ttcgaaatcc ggttcatggt cgccgagttc gaacgccgcc agtacgcgcc
    43381 cgtagtcggt gccatggctg cgatagtgga acaaagaaat gttccagtgc gtacccagcg
    43441 tgttgaggaa gcgcagcagc gcgcccggtg attccgggaa ttcgaagctg tagaggcgtt
    43501 cctgcaacgg atgtgacgga cgcccgccga ccatatagcg cacatgcaac ttcgccattt
    43561 cgtcgtcgga gagatccacc acgctgtagc cgccgtcgtt gagcatctgc aaaatttctt
    43621 tgcgctcttc gaggccgcgg ctcaggcgca cgccgacaaa gatgcaggcg tttttggcat
    43681 cggcaaaacg gtagttgaac tcggtgaccg atcgcccgcc aagcagttgg cagaatttga
    43741 ggaagctgcc tttttcttcc ggaatggtca ccgccaacaa cgcttcacgc tgttcgccca
    43801 gttcgcagcg ttccgagacg tagcgcaggc cgtggaagtt cacgttggca ccggaaagaa
    43861 tatgcgccag ccgttcgccg cgaatgttgt gctgggcgat atattttttc attcccgcca
    43921 gcgccagcgc gccagagggt tccgccaccg cgcgcacatc ttcgaataaa tccttcatcg
    43981 ccgcacagat cgcatcacta tcgacggtga tgatgtcgtc gagatactcc tggcataaac
    44041 ggaaggtttc gtcaccgatg cgttttaccg ctacgccttc agcaaatagc cctacgcgcg
    44101 gcagatcaac cggatgaccc gcatccagcg ctgctttcag gcaggcggag tcttccgctt
    44161 ctacggcgat cactttgatt tgtggcatca gttgtttgat cagcaccgca acgcccgccg
    44221 ccagaccgcc gccgccgact ggcacaaata cgcggtcgag atgggcgtcc tgctggagca
    44281 gttccagcgc cagcgtgcct tgcccggcaa tcaccatcgg atggtcgaac ggcggcaccc
    44341 aggtgaaccc ctgctgctgt gacagttcga tcgctttagc tttcgcttcg tcaaagttcg
    44401 cgccatgaag cagcacttcg ccgccgaatc cacgtaccgc atcgactttg atgtcggcgg
    44461 tggcggttgg cataacgatc agggccttca cgcctaaccg cgcagaagaa aacgcgacgc
    44521 cctgcgcgtg gttacccgca gaagcagtga tcacgccgtg cgctttttgt tcttccgtca
    44581 ggcccgccat catggcgtat gcgccgcgca gcttaaagct gtgcactggc tggcgatctt
    44641 cgcgcttcac cagaatgacg ttatcaagac gcgacgacag tttttccatt ttttgtagcg
    44701 gcgtaacctg cgccgcctcg taaaccggcg cgcgcagcac tgctcttaaa tattcggcac
    44761 cttccggagc accggacagg ggttgcgagt cagccattat taacccccca gtttcgattt
    44821 atcgcgcacc gcacctttgt cggcgctggt tgccaggctg gcgtaggcgc gcagggcaaa
    44881 ggaaacctga cgttcacggt ttttcggcgt ccaggctttg tcaccccgag cttcctgcgc
    44941 ttcacgacgc gccgccagtt cggcatcgct tacctgtaac tgaatgccac ggttcgggat
    45001 gtcgatagcg atcaggtcac catcttcaat cagaccaatg ctgccgccgc ttgccgcttc
    45061 cggtgagacg tggccgatgg aaagaccaga ggtgccgcca gagaaacgac cgtcggtgat
    45121 cagcgcacag gctttaccga gacccattga tttcaggaag ctggttgggt agagcatttc
    45181 ctgcatgcca gggccgcctt tcgggccttc atagcggatc accaccacat cgcccgcgac
    45241 aactttgcca ccgaggatcg cttctaccgc gtcgtcctgg ctttcgtaca ctttcgccgg
    45301 gccggtgaat ttgaggatgc tgtcatcgac gcccgcggtt ttaacgatgc agccgttttc
    45361 tgcgaaatta ccgtagagca ccgccaggcc gccgtctttg ctgtaggcgt gttccagcga
    45421 gcggatacag ccattggcgc gatcgtcatc gagagaatcc caacggcaat cctgcgagaa
    45481 tgcctgtgtg gtacgaatgc ctgcaggacc tgcgcggaac atatttttta ccgcgtcatc
    45541 ctgggtcagc ataacgtcgt attgttccag cgtttgcggc aacgtcaggc caagtacgtt
    45601 tttcacatca cggttcagta accccgcgcg atccagttcg ccgagaatac cgataacacc
    45661 accagcacgg tgaacatctt ccatatggta tttctgggtg ctcggcgcaa ctttacacag
    45721 ctgtggaacc ttgcgggaaa gcttatcgat atcactcatg gtgaagtcga tttccgcttc
    45781 ctgcgccgcc gccagcaggt gaagtacggt gttagtcgat ccacccatcg cgatatccag
    45841 cgtcatggcg ttttcaaacg ccgctttgct agcaatattg cgcggcagtg cactttcgtc
    45901 gttttgctcg tagtaacgtt tggtcaattc aacaatgcgt ttaccggcat tgaggaacaa
    45961 ctgcttacgg tcggcgtggg ttgccagcag cgagccgttg cccggctgcg acagacccag
    46021 cgcttcggtc aggcagttca ttgagttagc ggtaaacatc ccggagcagg agccgcaggt
    46081 cggacacgcg gaacgttcaa cctgatcgct ctgggagtca gaaactttcg ggtctgcgcc
    46141 ctggatcatc gcatcaacca gatcgagttt gattatctga tcggacagtt tggttttccc
    46201 ggcctccatc gggccgccgg aaacaaagat caccggaata ttcaggcgca gggaagccat
    46261 cagcatcccc ggggtgattt tgtcgcagtt agagatgcag accatggcgt cggcgcagtg
    46321 ggcgttgacc atatactcaa cggaatcagc gatcagttcg cgagatggca gtgaataaag
    46381 catccccccg tggcccatgg caatcccatc atccaccgca atggtgttga actctttggc
    46441 aacgccgcca gccgcttcaa tttgttcggc gaccagttta ccgagatcgc gcagatggac
    46501 gtgacccggt acaaattggg tgaacgagtt cacaaccgcg ataatcggct taccgaaatc
    46561 ggcgtcggtc attccggtgg cgcgccacag cgcacgagca cccgccatat tacgaccatg
    46621 agtggtggtg gcggaacggt acttaggcat actttattta ctcccagtgt ctgtctcgta
    46681 aatgggacgg tgcgtgccgt cccatttttt gtatttattg attaacttga tctaaccagc
    46741 cccatttatc ttcggtttcg ccagtgaaga ggccgaagaa ggcttgctga atgcgtttgg
    46801 taaccgggcc acaacggcct tcgccaacct gaataccgtc tacgctgcgc actggcgtga
    46861 tttctgccgc cgtaccggac ataaacactt catccgccag gtacagggat tcgcgcgaca
    46921 gaacctgctc acgcacatca attcccagct ctttcgccag tttgatgatg gcatcacggg
    46981 taatacccgg cagcgcggag gaggtgaacg gtggggtgaa cagcacacca tctttcactt
    47041 caaacaggtt ttcgcctgcg ccttcagaga tataaccgtt cacatccagc gcgatacctt
    47101 cctgataacc gtggcggcgc gcttcgctac ccaccagcag ggaagagagg tagttaccac
    47161 cggcttttgc cgccgtcggg atggtgtttg gtgctgcgcg gttccaggag gaaaccatcg
    47221 catcgatccc ctgctccagc gcttctgcgc ccagatacgc tccccacggg aaagcagcga
    47281 taatcacgtc ggttgagtat cccgctggcg ggtttactcc catgccaaca tcaccgacga
    47341 agatcagcgg acggatatag gcgctggtga gattattttt gcggatcacg tcacgacaag
    47401 cttccatcag ctcatcaatg ctctgtgaaa ccgggaagcg atagattttg gcggagtcat
    47461 gcagacgctg catatgctca cgatggcgga atacaaccgg tcctttgtgt gagtcgtagc
    47521 aacggatgcc ttcaaaaacc gaggtgccat agtgcagcgc gtgcgacatc acatgcacct
    47581 tcgcgtcttc ccagcgaacc atctccccat tgaaccaaat gtaatcagct ttcttcgtgg
    47641 tcatttttat attccttttg cgctcaggcg cggatttgtt gtgatgtggt tgtgctctgg
    47701 cagatggcaa cgtgtgcgac gtccaccagt ttatttaact gactaaacag taagtcgacc
    47761 gaccgtgggc tggcaacggt caattcgata tttatatttt gtgcatcgct ggcggcagcc
    47821 atattcattg agcagacgtg gaaaccacga tgacgcacca cgcgtaaaac acgttctaag
    47881 gtttccggat tgaagcgagc cgatacattg acctgatgtt gcatcatgat aatttctcca
    47941 acatttctga attactggcg ccaggcggca ccagcggcca gacgttctca agttcgtcga
    48001 ttgagacatg aagcaggtat ggcccatcac tgttcagcat ggtgtcgagt gccgcttcaa
    48061 cctggtcttt acgggtgatg tgttggccag ggatgccgaa ggcgctggct aacatgagga
    48121 aatcggggtt atcagtaagg gtggtttcgc tgtatcgttc ctgaaaaaac agttgctgcc
    48181 attgtcgaac catccctaac cgttggttat cgagtaagac gattttcaac ggtaactgct
    48241 tgcgttttac ggtgcccagc tcttgcacat tcatcatgaa agagccgtca ccggagatac
    48301 agacgacagt atcgttcggt cgtgcgactt gtgcgccaac cgccgctggt aaaccgaaac
    48361 ccatggtgcc taagccgctg gaggtaatga aattttccgg gcgagtgtgt gcgatgtgct
    48421 gcgcggccca catctggtgc tgccccacat ctgtggtcac gacgcaatcc gcaggtttac
    48481 gatccgacag ttgttttaac aacaatggcg cgtagatagc gtcaccggga tggtcgtaac
    48541 gccaggcatg ttcatcacgc agctgcgcgc agtgtagctg ccagtcattg atatttaacg
    48601 gctgctgtaa tgctggtaac agagcattta aatcaccttg taatgccaca tgtgcctgac
    48661 gcagcttgtt catttctgcc gggtcgatat ccatatggat aacactggcg tgtggtgcga
    48721 aggtgttcag tttgccggtc acccggtcat caaaacgtgc acccacggcg atcagcaagt
    48781 cgcactcctg caccgcgaag ttcgccgctt tggtgccatg cattcccagc atgcccagat
    48841 agtacggata atctgcttca actgcgccca gccctttcag cgtgcaggtg gcaggcattt
    48901 ttgtggtagc gagaaattct cgtaaagcag gaactgcctg cgccataccc acgccaccac
    48961 caacgtacag catcggtttt tgcgcttttg ccagcatctg gcgcgcttgc tcaacttcgg
    49021 catgtgggaa agtcacttcg ttttcaacgg tggtgaacca cggttccagg tcaccgctgg
    49081 ctagctggat atcttttggg atatcgacca gaaccggacc aggacgacct gagctggcaa
    49141 cgtcgaatgc ttcagccata atgcgcggca actcttccag cgactgcacc agaaagctgt
    49201 gcttggtaca ggctaacgac aatcccagga catccacttc ctgaaatgcg tccgtgccga
    49261 taaacggtgc ggacacttga ccggtgatgg caacaacagg gatagaatct aacagtgcgt
    49321 ccgcaagccc ggttatcagg ttggttgcgc ccggaccaga cgtggcgata catacgccag
    49381 ttttgccggt agcacgggca taaccgatag ccgccattgc cgcaccctgc tcatgtcggc
    49441 acagcaagtg ctccacgccg ccgtcataca atgcatcgta aaccggcata attgcgccac
    49501 ccggataacc gaaaacggtg ttcacaccct gtgcccgcaa cgcatgtacc acccactgtg
    49561 cgccattcat agttagttcc ccgtcctgaa tcttgagaaa cagaattttg tgctgttatt
    49621 cattgtctgc tcctcggtta tgtttttaag gtcaaaaaaa acccccggac ctttcggtgc
    49681 gggggtctta gttcgttaag gcttgatctc taagcctttc ctcgtccaag tgcagccccg
    49741 cacggtggga taataatcac caccacgcta atcacgacca ggctaatcac tcgtagaagg
    49801 gctgtcattt tgtcttttct tgcatcttgt tcgaaggaat gcctaaagag ttaccataga
    49861 ttttgcgaat agcacaagat attttttggc cgatttttat gatgcgttta ataatttcaa
    49921 tagaaattgt tgttttttaa aaagaaaata gtgagtgaaa aaaaattcaa tttcagtatg
    49981 aatgttgttc ccttcctcgt agttcatcct ttgtccgctg catttttgcg agcatcattc
    50041 caccgccaga acaactcaac ttcacttgca caaataaaca ggaatcagcc actaaaattc
    50101 acgaattagc gggctacagc agcgatatct tatgcacata atagtgactc aaggagggct
    50161 tatgtcactg tcaattgttc atacccgcgc agccctggga gtaaatgcgc ccccaatcac
    50221 tgttgaggta catatcagta aaggtctacc cggcttaacg atggtgggct taccagaaac
    50281 aactgtaaaa gaagctcgcg atcgcgtgcg cagcgccatt atcaatagcg gatatgaata
    50341 tccggcgaaa aaaatcacca tcaacctggc tccagccgat ctgccaaaag aagggggacg
    50401 atatgattta cctatcgcca ttgcgttgct ggcggcctca gaacagctta cagccaataa
    50461 gttagatgaa tatgaattag tcggagaact ggcgcttaca ggcgctctgc gtggcgttcc
    50521 cggcgcaatc tccagtgcaa ctgaagctat taagtcgggc agaaaaatta tcgtcgcgaa
    50581 agataacgaa gatgaagtgg ggctaattaa cggtgaagga tgcctgatag ccgatcatct
    50641 gcaggctgtc tgtgcgtttc tggaaggtaa gcacgctctc gaacgcccga aaccaactga
    50701 tgcagtatcc cgggcgctac aacatgatct cagtgatgtt gtcggtcagg agcaaggaaa
    50761 gcgaggactg gaaattaccg ccgctggcgg gcacaacctt ttactgattg ggccgccggg
    50821 aacaggtaaa acaatgctcg ccagccgtat taatggcctg ttgccagatt taagcaatga
    50881 agaggcactg gagagcgctg cgatattaag tctggtaaat gctgaatcag tacaaaaaca
    50941 atggcggcag cgcccgttcc gctcaccgca tcacagtgcg tcgttaactg cgatggtggg
    51001 cggtggtgca attccagggc caggtgaaat ttcgttggcg cataacggcg tgctttttct
    51061 tgatgagcta cctgaatttg aacggcgtac actggatgcc ttgcgagagc cgattgaatc
    51121 cgggcagatc catctttcac gcacacgagc caaaataacc tatccagccc gtttccagct
    51181 tgttgcggcg atgaatccca gccctaccgg acattatcag ggaaaccata accgctgcac
    51241 gccagaacag acattacgtt atctcaaccg gctctcgggg ccctttctcg accgcttcga
    51301 tctctcactg gagatcccat taccaccccc cggcattttg agtaaaacgg tagtgccggg
    51361 agaaagcagc gccaccgtta aacaacgcgt aatggccgcc agagagcgcc aatttaagcg
    51421 gcagaataaa ctgaatgcct ggctggatag tccggaaata cgccaattct gcaaacttga
    51481 gagcgaagat gcgatgtggt tggaaggaac actgatccat ctggggttat cgattcgtgc
    51541 ctggcagcgg ttattgaaag ttgcacgaac cattgctgat attgatcagt ctgacattat
    51601 cacacgtcag catttgcagg aggcagttag ctatcgtgcg attgaccgtt tgctcatcca
    51661 tctgcaaaaa ctactgacat aaaaaaaggg catttcgccc tttttattaa tcatcagaat
    51721 cggtgtagtc ttcagcacct tcaacctgcg gtttaccgcc ggaaagggtg tgaaaacgtt
    51781 ttggacgctt aatacgcgtc atatacttgg accacacgcg ttctgcttct gtcactggct
    51841 cacgttcgcc acggcatact gctacgaaga gtttctcttc ctcggtaacc ggctcgcgtt
    51901 tgccaagatc caactcattg aaggcataac catgacgctc aagcagttgt gcctctttga
    51961 tggtgaaatc accatgacga gagaatccac gtggataatg tttattgtcg aaatatcgat
    52021 tagtcgtcgt aaagctttcc gccatcctgc acgctcctaa ttctttgacc gagctagtta
    52081 tggcgcggag tattagttac gcttgacaga gtgtaaaaca aaacatttaa atcataacga
    52141 caaataattt tgcggagagc actgtggata cggaattgtt aaaaactttc ctggaagtta
    52201 gccgaacgcg tcactttggt cgagcggctg aatcgctcta tctgacccag tcagcagtga
    52261 gctttcgaat cagacaactg gaaaatcaac tgggtgtgaa ccttttcacc cgccacagaa
    52321 acaatatccg tttaaccgct gccggtgaaa aactactgcc ttatgcagaa acgctcatga
    52381 gcacctggca ggccgcccgc aaggaggtgg cgcatacctc acgacataac gagttttcta
    52441 tcggtgccag cgcctcattg tgggaatgta tgcttaatca gtggctggga cgtttgtatc
    52501 aaaatcagga tgcccataca ggcttacagt tcgaagcgcg aattgcccaa cggcagtctc
    52561 tggtaaaaca gctgcatgaa cgccagcttg atcttcttat taccactgaa gcgcccaaaa
    52621 tggacgaatt tagtagtcag ttgctgggat atttcacttt agcgctttat accagtgccc
    52681 cttcaaaact aaagggagat cttaattatc tgcgacttga gtgggggcca gattttcaac
    52741 agcatgaggc aggtttgatc ggtgctgacg aagtgcccat tctgacaacc agttctgctg
    52801 aactggcaca gcaacagatt gcgatgctta atggttgcac ctggctaccc gtcagctggg
    52861 cgcgtaaaaa aggcggcctg cataccgttg tcgatagcac aacactttca cggccgcttt
    52921 atgccatatg gctgcaaaat agcgataaaa atacgttgat tcgcgatctt ttgaaaatta
    52981 acgtgctgga tgaagtgtat taatatgaat ggctggcaag gatgccggta gaaggattta
    53041 cttcggagag ggttatttca gataaaaaaa atccttagct ttcgctaagg atgatttctg
    53101 gcaggggcgg agagactcga actcccaaca cccggttttg gagaccggtg ctctaccaat
    53161 tgaactacgc ccctaattag
//
LOCUS       DAZFXT010000016        52567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.016, whole genome shotgun sequence.
ACCESSION   DAZFXT010000016 DAZFXT010000000
VERSION     DAZFXT010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 52567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.016"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..91)
                     /gene="yojO"
                     /locus_tag="ACHKW0_001090"
     CDS             complement(<1..91)
                     /gene="yojO"
                     /locus_tag="ACHKW0_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YojO"
                     /protein_id="HFO7473961.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISN"
     gene            200..694
                     /gene="napF"
                     /locus_tag="ACHKW0_001091"
     CDS             200..694
                     /gene="napF"
                     /locus_tag="ACHKW0_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HFO7473962.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDWQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            684..947
                     /gene="napD"
                     /locus_tag="ACHKW0_001092"
     CDS             684..947
                     /gene="napD"
                     /locus_tag="ACHKW0_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HFO7473963.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            944..3430
                     /gene="napA"
                     /locus_tag="ACHKW0_001093"
     CDS             944..3430
                     /gene="napA"
                     /locus_tag="ACHKW0_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HFO7473964.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3437..4132
                     /gene="napG"
                     /locus_tag="ACHKW0_001094"
     CDS             3437..4132
                     /gene="napG"
                     /locus_tag="ACHKW0_001094"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HFO7473965.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4119..4982
                     /gene="napH"
                     /locus_tag="ACHKW0_001095"
     CDS             4119..4982
                     /gene="napH"
                     /locus_tag="ACHKW0_001095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HFO7473966.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4979..5428
                     /gene="napB"
                     /locus_tag="ACHKW0_001096"
     CDS             4979..5428
                     /gene="napB"
                     /locus_tag="ACHKW0_001096"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HFO7473967.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5438..6040
                     /gene="napC"
                     /locus_tag="ACHKW0_001097"
     CDS             5438..6040
                     /gene="napC"
                     /locus_tag="ACHKW0_001097"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HFO7473968.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6053..6676
                     /gene="ccmA"
                     /locus_tag="ACHKW0_001098"
     CDS             6053..6676
                     /gene="ccmA"
                     /locus_tag="ACHKW0_001098"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HFO7473969.1"
                     /translation="MGMLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTT
                     LLRLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHR
                     DGDTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAID
                     VNGVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6673..7335
                     /gene="ccmB"
                     /locus_tag="ACHKW0_001099"
     CDS             6673..7335
                     /gene="ccmB"
                     /locus_tag="ACHKW0_001099"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HFO7473970.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7377..8114
                     /gene="ccmC"
                     /locus_tag="ACHKW0_001100"
     CDS             7377..8114
                     /gene="ccmC"
                     /locus_tag="ACHKW0_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HFO7473971.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8111..8320
                     /gene="ccmD"
                     /locus_tag="ACHKW0_001101"
     CDS             8111..8320
                     /gene="ccmD"
                     /locus_tag="ACHKW0_001101"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HFO7473972.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8317..8796
                     /gene="ccmE"
                     /locus_tag="ACHKW0_001102"
     CDS             8317..8796
                     /gene="ccmE"
                     /locus_tag="ACHKW0_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HFO7473973.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8793..10736
                     /gene="ccmF"
                     /locus_tag="ACHKW0_001103"
     CDS             8793..10736
                     /gene="ccmF"
                     /locus_tag="ACHKW0_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HFO7473974.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLCGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10733..11290
                     /gene="dsbE"
                     /locus_tag="ACHKW0_001104"
     CDS             10733..11290
                     /gene="dsbE"
                     /locus_tag="ACHKW0_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HFO7473975.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11287..12339
                     /gene="ccmH"
                     /locus_tag="ACHKW0_001105"
     CDS             11287..12339
                     /gene="ccmH"
                     /locus_tag="ACHKW0_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HFO7473976.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12374..13021)
                     /gene="narP"
                     /locus_tag="ACHKW0_001106"
     CDS             complement(12374..13021)
                     /gene="narP"
                     /locus_tag="ACHKW0_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HFO7473977.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13341..15932
                     /gene="yejO"
                     /locus_tag="ACHKW0_001107"
                     /pseudo
     CDS             13341..15932
                     /gene="yejO"
                     /locus_tag="ACHKW0_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16036..16112)
                     /locus_tag="ACHKW0_001108"
     tRNA            complement(16036..16112)
                     /locus_tag="ACHKW0_001108"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16076..16078),aa:Pro,seq:ggg)
     gene            complement(16187..17947)
                     /gene="yejM"
                     /locus_tag="ACHKW0_001109"
     CDS             complement(16187..17947)
                     /gene="yejM"
                     /locus_tag="ACHKW0_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS biosynthesis-modulating metalloenzyme YejM"
                     /protein_id="HFO7473978.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(17967..18194)
                     /gene="yejL"
                     /locus_tag="ACHKW0_001110"
     CDS             complement(17967..18194)
                     /gene="yejL"
                     /locus_tag="ACHKW0_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HFO7473979.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18376..19383
                     /gene="yejK"
                     /locus_tag="ACHKW0_001111"
     CDS             18376..19383
                     /gene="yejK"
                     /locus_tag="ACHKW0_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HFO7473980.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19522..19806)
                     /gene="rplY"
                     /locus_tag="ACHKW0_001112"
     CDS             complement(19522..19806)
                     /gene="rplY"
                     /locus_tag="ACHKW0_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HFO7473981.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19931..21691)
                     /gene="radD"
                     /locus_tag="ACHKW0_001113"
     CDS             complement(19931..21691)
                     /gene="radD"
                     /locus_tag="ACHKW0_001113"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HFO7473982.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21840..22535
                     /gene="rsuA"
                     /locus_tag="ACHKW0_001114"
     CDS             21840..22535
                     /gene="rsuA"
                     /locus_tag="ACHKW0_001114"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HFO7473983.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22563..23753
                     /gene="bcr"
                     /locus_tag="ACHKW0_001115"
     CDS             22563..23753
                     /gene="bcr"
                     /locus_tag="ACHKW0_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HFO7473984.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCASSSILFCLYASR
                     PKKR"
     gene            24086..24430
                     /gene="yejG"
                     /locus_tag="ACHKW0_001116"
     CDS             24086..24430
                     /gene="yejG"
                     /locus_tag="ACHKW0_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejG family protein"
                     /protein_id="HFO7473985.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(24434..26023)
                     /gene="yejF"
                     /locus_tag="ACHKW0_001117"
     CDS             complement(24434..26023)
                     /gene="yejF"
                     /locus_tag="ACHKW0_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HFO7473986.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(26025..27050)
                     /gene="yejE"
                     /locus_tag="ACHKW0_001118"
     CDS             complement(26025..27050)
                     /gene="yejE"
                     /locus_tag="ACHKW0_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7473987.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            complement(27050..28144)
                     /gene="yejB"
                     /locus_tag="ACHKW0_001119"
     CDS             complement(27050..28144)
                     /gene="yejB"
                     /locus_tag="ACHKW0_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HFO7473988.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMIWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            complement(28145..29959)
                     /gene="yejA"
                     /locus_tag="ACHKW0_001120"
     CDS             complement(28145..29959)
                     /gene="yejA"
                     /locus_tag="ACHKW0_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HFO7473989.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            complement(30041..31597)
                     /gene="pdeN"
                     /locus_tag="ACHKW0_001121"
     CDS             complement(30041..31597)
                     /gene="pdeN"
                     /locus_tag="ACHKW0_001121"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HFO7473990.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLITDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            complement(31778..32344)
                     /gene="mepS"
                     /locus_tag="ACHKW0_001122"
     CDS             complement(31778..32344)
                     /gene="mepS"
                     /locus_tag="ACHKW0_001122"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HFO7473991.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            complement(32756..33469)
                     /gene="lpxT"
                     /locus_tag="ACHKW0_001123"
     CDS             complement(32756..33469)
                     /gene="lpxT"
                     /locus_tag="ACHKW0_001123"
                     /EC_number="2.7.4.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HFO7473992.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VLLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            complement(33508..34494)
                     /gene="yeiR"
                     /locus_tag="ACHKW0_001124"
     CDS             complement(33508..34494)
                     /gene="yeiR"
                     /locus_tag="ACHKW0_001124"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HFO7473993.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            complement(34612..36078)
                     /gene="yeiQ"
                     /locus_tag="ACHKW0_001125"
                     /pseudo
     CDS             complement(34612..36078)
                     /gene="yeiQ"
                     /locus_tag="ACHKW0_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(36301..36873)
                     /gene="yeiP"
                     /locus_tag="ACHKW0_001126"
     CDS             complement(36301..36873)
                     /gene="yeiP"
                     /locus_tag="ACHKW0_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA];
                     GO_process: GO:0043043 - peptide biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HFO7473994.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            37028..37282
                     /gene="yeiW"
                     /locus_tag="ACHKW0_001127"
     CDS             37028..37282
                     /gene="yeiW"
                     /locus_tag="ACHKW0_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7473995.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            complement(37279..38457)
                     /gene="setB"
                     /locus_tag="ACHKW0_001128"
     CDS             complement(37279..38457)
                     /gene="setB"
                     /locus_tag="ACHKW0_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HFO7473996.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMGTAAGLEIPTMLIAGYFAK
                     RLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLMP
                     GQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKDV
                     "
     gene            38825..39955
                     /gene="fruB"
                     /locus_tag="ACHKW0_001129"
     CDS             38825..39955
                     /gene="fruB"
                     /locus_tag="ACHKW0_001129"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HFO7473997.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSAPTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            39955..40893
                     /gene="fruK"
                     /locus_tag="ACHKW0_001130"
     CDS             39955..40893
                     /gene="fruK"
                     /locus_tag="ACHKW0_001130"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008662 - 1-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HFO7473998.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            40910..42601
                     /gene="fruA"
                     /locus_tag="ACHKW0_001131"
     CDS             40910..42601
                     /gene="fruA"
                     /locus_tag="ACHKW0_001131"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HFO7473999.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATSE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            43025..43966
                     /gene="psuK"
                     /locus_tag="ACHKW0_001132"
     CDS             43025..43966
                     /gene="psuK"
                     /locus_tag="ACHKW0_001132"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HFO7474000.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRGDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            43954..44892
                     /gene="psuG"
                     /locus_tag="ACHKW0_001133"
     CDS             43954..44892
                     /gene="psuG"
                     /locus_tag="ACHKW0_001133"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HFO7474001.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            44986..46236
                     /gene="psuT"
                     /locus_tag="ACHKW0_001134"
     CDS             44986..46236
                     /gene="psuT"
                     /locus_tag="ACHKW0_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7474002.1"
                     /translation="MDIMRSVVGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(46307..47005)
                     /gene="yeiL"
                     /locus_tag="ACHKW0_001135"
     CDS             complement(46307..47005)
                     /gene="yeiL"
                     /locus_tag="ACHKW0_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HFO7474003.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLTKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            47135..48076
                     /gene="rihB"
                     /locus_tag="ACHKW0_001136"
     CDS             47135..48076
                     /gene="rihB"
                     /locus_tag="ACHKW0_001136"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HFO7474004.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            48176..49426
                     /locus_tag="ACHKW0_001137"
     CDS             48176..49426
                     /locus_tag="ACHKW0_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HFO7474005.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(49482..50570)
                     /locus_tag="ACHKW0_001138"
     CDS             complement(49482..50570)
                     /locus_tag="ACHKW0_001138"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HFO7474006.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(50574..51431)
                     /gene="nfo"
                     /locus_tag="ACHKW0_001139"
     CDS             complement(50574..51431)
                     /gene="nfo"
                     /locus_tag="ACHKW0_001139"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HFO7474007.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(51505..52554)
                     /gene="yeiH"
                     /locus_tag="ACHKW0_001140"
     CDS             complement(51505..52554)
                     /gene="yeiH"
                     /locus_tag="ACHKW0_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HFO7474008.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
BASE COUNT        12647 a        12885 c        14183 g        12852 t
ORIGIN      
        1 catttgaaat gtgagcaaaa gcccgttttt gccacgcttc ccgccactct tttgatcctg
       61 ctacaggttt taccccgatc ggggtatgca tctttgacac atcctttaat atcttagcgg
      121 ctataaaaat ggcttattaa ttatgcggtt tatttggtcg ctctcaattt tcagagcgcg
      181 ttaatgatgg aaggtcaatg tgaagattga tgcatcccgt cggggcatac tcactggtcg
      241 ctggcgcaaa gccagtaacg gtatccgtcc gccctggtcc ggtgatgaat ctcattttct
      301 gacccattgc acccgctgcg acgcctgtat taatgcttgt gaaaacaaca ttctgcaacg
      361 cggcgcgggc ggctatccga gcgttaattt caaaaataac gagtgcagct tctgttacgc
      421 ctgcgcgcag gcttgccccg aatcgctatt ttctccgcgc cacaccaggg cctgggattg
      481 gcagttcacc attggggacg cgtgcctggc gtatcagtct gttgaatgcc gccgctgtca
      541 ggatagctgt gagccaatgg caattatctt tcgcccgacg ctctccggga tctaccagcc
      601 gcaacttaat agccaacttt gcaacggatg tggagcatgt gcagccagct gtccggtatc
      661 agccataact gcggagtatc tccatgcaca ctaactggca agtttgcagc ctggtcgtgc
      721 aggccaaaag cgaacgaatt tcagacatca gcacccaact gaacgccttt cccggctgtg
      781 aagttgctgt cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa gcagaagaca
      841 gcgaaacgct gatccaaacc attgagtcag tacgcaacgt agagggcgtg ctggcggtgt
      901 cgctggttta tcaccagcag gaagagcaag gtgaggaaac accatgaaac tcagtcgtcg
      961 tagctttatg aaagctaacg ccgttgcggc cgctgcggcg gctgccggtc tcagcgtgcc
     1021 gggcgttgcc cgcgccgttg ttggtcagca ggaagccatc aaatgggata aagcgccgtg
     1081 ccgtttctgc ggtactggtt gcggcgttct ggtcggaacg cagcaggggc gtgtggtggc
     1141 ctgtcagggc gacccggacg caccggttaa ccgtggcctg aactgcatta agggctattt
     1201 cctgcccaag atcatgtacg gtaaagaccg tttgacgcag ccgctgctgc gtatgaaaaa
     1261 cggtaaatat gacaaagaag gcgaatttac cccaatcacc tgggatcagg ccttcgatgt
     1321 gatggaagag aaattcaaaa ccgccctgaa agaaaaagga ccggaatcga tcggtatgtt
     1381 cggttctggt cagtggacta tctgggaagg ttatgccgcg tccaagctgt tcaaagcggg
     1441 cttccgttcg aacaacatcg acccgaacgc acgtcactgt atggcgtcgg cagtagttgg
     1501 ctttatgcgt acctttggta tggatgagcc gatgggctgc tatgacgaca tcgagcaggc
     1561 tgacgcgttt gtgctgtggg gcgctaacat ggcggagatg cacccgatcc tctggtcacg
     1621 catcactaac cgtcgtctct ctaaccagaa cgtcaccgtg gcggtgcttt ctacctacca
     1681 gcatcgtagc ttcgagctgg cggataacgg catcatcttt acgccgcaat ctgacctggt
     1741 gatcctgaac tacatcgcca actatatcat tcaaaacaat gcgataaatc aggacttctt
     1801 cagcaagcac gttaacctgc gcaaaggggc gacggacatc ggctacggtt tacgtccgac
     1861 ccatccgctg gaaaaagcag cgaagaatcc aggttctgac gcctccgaac cgatgagctt
     1921 tgaagattac aaagccttcg ttgccgagta tacgctggaa aaaactgccg aaatgaccgg
     1981 cgtgccgaaa gaccagttag aacaactggc gcagctgtat gccgatccga acaagaaagt
     2041 catctcctac tggacgatgg gcttcaacca gcatactcgt ggcgtgtggg ccaacaacct
     2101 ggtctacaac ctgcacctgc tgaccggcaa aatttcccag ccgggttgcg gtccgttctc
     2161 cctgaccggg cagccttccg cgtgtggtac tgcgcgtgaa gtgggcacct ttgctcaccg
     2221 tctgcctgcg gacatggtgg tgactaacga gaaacaccgt gatatttgcg agaagaagtg
     2281 gaatatcccg agcggcacca ttccggcgaa aatcggtctg cacgcggtgg cacaagaccg
     2341 tgcactgaaa gacggcaagc tgaatgttta ctggaccatg tgtaccaaca acatgcaggc
     2401 cgggccgaac attaatgaag agcgtatgcc gggctggcgc gatccgcgca acttcatcat
     2461 cgtctccgat ccgtatccga cagtcagtgc gctggcagcc gacttgatcc tgccgaccgc
     2521 aatgtgggta gagaaagagg gcgcttacgg taacgccgaa cgccgtactc agttctggcg
     2581 tcagcaggta caggcgccag gcgaagcgaa atcggatctc tggcagttag tccagttctc
     2641 ccgccgcttc aaaactgaag aagtatggcc ggaagagctg ctggcgaaga aaccggaact
     2701 gcgtggcaaa acgctgtacg aagttctgta tgccaccccc gaagtgagca aattcccggt
     2761 atccgaactg gcggaagatc agctgaacga tgaatcccgc gagctgggct tctatctgca
     2821 aaaagggctg ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg atctcgcgcc
     2881 gttcgatgac taccacaaag cgcgcggtct gcgctggccg gtggtgaacg gtaaagaaac
     2941 acagtggcgt tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag gctataagtt
     3001 ctacggtaaa ccggatggca aagcggtgat cttcgcactg ccgttcgaac cggcggcgga
     3061 agcaccggat gaagagtacg acctgtggct ctctaccgga cgcgttctgg agcactggca
     3121 caccggcagt atgactcgcc gtgtgccgga actgcaccgc gccttcccgg aagcggtctt
     3181 gtttattcac ccgctggatg cgaaagcgcg cgatctgcgc cgtggcgaca aagtgaaagt
     3241 ggtttctcgc cgtggtgaag tgatctcgat tgttgaaacg cgcggtcgta accgtccgcc
     3301 acagggcctg gtgtacatgc cgttcttcga cgccgcacag ctggttaaca aactgacgct
     3361 ggatgcgacc gatccgctct cgaaagagac ggacttcaag aagtgcgcag tcaaactgga
     3421 gaaggtgtaa gccattatgt cccggtcagc gaaacctcaa aatggtcgcc gccgctttct
     3481 gcgcgatgtc gttcgcacag caggcgggct ggctgccgtg ggtgtggcgc tggggttaca
     3541 acagcaaacc gcacgcgcat ctggcgtgcg gttgcgcccg cccggagcca taaacgagaa
     3601 cgcctttgcc agtgcctgtg tgcgttgcgg tcagtgtgtt caggcttgcc cttacgacac
     3661 cttaaaactg gcgacgctgg cctctggtct gtcggcgggc acgccatatt ttgtcgcacg
     3721 ggatattcct tgcgaaatgt gtgaggacat tccgtgcgcc aaagtgtgcc caagcggtgc
     3781 gctggatcgt gagattgaat cgatcgacga cgcgcggatg gggctggcgg tactggtgga
     3841 ccaggaaaac tgtctcaact ttcaggggct gcgctgcgat gtttgttatc gcgaatgccc
     3901 gaaaattgat gaggccatca ccctggagct ggagcgcaac acgcgtaccg gtaagcacgc
     3961 ccgctttctg ccgacggttc acagcgacgc ctgtaccggt tgcggtaagt gcgaaaaagt
     4021 atgcgtgctg gaacaaccgg caatcaaggt gttaccgctg tcactggcga aaggggagtt
     4081 aggtcaccat taccgcttcg gttggctgga ggggaacaat ggcaaatcgt aaacgtgacg
     4141 ccgggcgcga ggcgctggag aaaaaaggct ggtggcgcag tcaccgttgg ctggtgttac
     4201 gtcgcctttg tcagttcttc gtgctgggga tgtttttgag cggtccatgg tttggtgtgt
     4261 ggatcttgca cggcaactac agcagtagcc tgttattcga caccgttccg ctgaccgatc
     4321 cgctgatgac gctgcaaagt cttgccagtg gtcatctgcc tgccacggtg gcgttgaccg
     4381 gtgcggtgat tatcaccgtg ctctacgccc tggcgggaaa gcgattattt tgcagctggg
     4441 tctgcccgct gaacccgatt accgacttag cgaactggtt acgcaggcgc tttgacctca
     4501 atcagtctgc gacgatccct cgccacattc gctacgtgct gttggtggtc attctgctgg
     4561 gatcggcctt aactggcacg ctcatctggg aatggatcaa ccccgtatct ctgatggggc
     4621 gcagcctggt aatgggcttc ggcagcggcg cgctgcttat tctcgcgctg tttttatttg
     4681 atctactggt cgttgaacac ggctggtgcg ggcacatttg tccggttggc gcgctgtatg
     4741 gcgtgctggg tagcaaaggt gtgattaccg ttgcggccac cgatcggcag aaatgtaacc
     4801 gctgtatgga ttgttttcat gtttgcccgg aaccgcatgt gctacgtgcc ccggtgctgg
     4861 atgaacaaag cccggtgcag gtcaccagcc gcgattgcat gacgtgcggt cgctgcgtgg
     4921 atgtctgttc tgaggatgta tttacaataa ctacacgatg gagttcggga gcgaaatcat
     4981 gaaaagccat gacctgaaga aagcgctgtg tcaatggacg gcgatgctgg ccctggtggt
     5041 aagcggcgcg gtttgggcgg ctaatggcgt cgattttagc cagtcgccgg aagtatccgg
     5101 gacacaggaa ggggccattc ggatgccaaa agagcaggaa cggatgccgc tgaactatgt
     5161 gaatcagccg ccaatgatcc cgcatagcgt tgaaggttat caggtaacga ccaataccaa
     5221 ccgctgcttg cagtgccacg gtgtcgaaag ttatcgcacc actggcgcgc cgcgtatcag
     5281 tcctactcac tttatggaca gcgacggcaa agtgggcgcg gaagtggctc cgcgtcgtta
     5341 tttctgtctg caatgtcacg taccgcaggc cgataccgcg ccaatcgtgg ggaatacctt
     5401 taccccatca aaaggttacg ggaaataaga ggttattatg ggaaattctg accgtaagcc
     5461 tggtctgatt aagcgcctgt ggaaatggtg gcgtaccccc agccgtctgg cgctggggac
     5521 gctgctgttg atcggttttg ttggcggcat cgtcttctgg ggtggcttta acaccgggat
     5581 ggaaaaagcc aataccgaag agttctgcat tagctgccac gaaatgcgca acacggtgta
     5641 tcaggaatac atggattccg tgcactacaa caaccgtagc ggcgtccgtg cgacctgtcc
     5701 ggattgtcac gttccgcacg agtttgtgcc gaagatgata cgcaagctca aagcaagtaa
     5761 agagctgtat ggtaaaattt ttggcgttat tgacacgccg cagaaatttg aagctcatcg
     5821 tctgacgatg gcacagaatg agtggcggcg catgaaggac aataactcgc aggagtgccg
     5881 taactgtcac aacttcgagt atatggatac aaccgcccag aaatcggttg ccgcgaagat
     5941 gcatgaccag gcggtgaaag atgggcaaac ctgtattgat tgccataaag ggatagcgca
     6001 caagctgccc gatatgcgtg aagtcgagcc aggtttttaa cagggttatt gcgtgggtat
     6061 gcttgaagcc agagagttac tttgtgagcg ggatgaacga accttattta gtggcttgtc
     6121 atttacgctg aacgcaggag agtgggtaca aatcaccggt agcaacggcg cggggaagac
     6181 aacgcttctc cgtttgctga cggggttgtc tcgccctgac gcaggcgagg ttctctggca
     6241 agggcagccc ttgcatcagg tacgcgacag ctaccatcaa aacctgttat ggataggcca
     6301 tcagccgggg atcaaaaccc ggctgacggc gttagaaaat ctgcactttt atcatcgcga
     6361 tggcgatacc gcacaatgtc tggaagccct ggcgcaggcc gggcttgccg gattcgaaga
     6421 tattcctgta aatcagctct cggccgggca acaacgccgc gtcgctttag cgcgtctgtg
     6481 gctgacccgt gccacgttat ggatcctcga cgagcctttt accgcgattg acgttaacgg
     6541 cgtcgatcgt ctgacccagc gtatggcgca gcatacggag caggggggga ttgtgattct
     6601 gactacccac cagccgctca acgttgctga aagtaaaatt cgccgcattt cactgacgca
     6661 aacgagggcc gcatgatgtt ctggcgcatt ttccgtcttg agctgcgtgt agcgtttcgc
     6721 catagcgccg aaatcgccaa cccgctgtgg ttcttcctga ttgtaattac cctttttccg
     6781 ctcagtatcg gtccggagcc gcaactgctg gcgcgtattg caccgggcat tatctgggtt
     6841 gctgcgctgc tttcatcctt gctggcgctg gaacgactgt tccgtgacga tttgcaggac
     6901 ggcagtcttg aacaattgat gttgttgccg ttacccttgc ccgccgttgt gctggcgaag
     6961 gtgatggcgc actggatggt aaccggtctg ccgttactca ttctttcgcc actggtagca
     7021 atgctactgg gaatggatgt ttatggctgg caagtgatgg cgctgacgct gctgctggga
     7081 acgcctacgc ttggctttct cggtgcaccg ggcgtggcgc tgacagtggg acttaagcgc
     7141 ggtggtgtgc tgctcagcat actggtgtta ccgctgacta tcccattact catcttcgcc
     7201 accgccgcga tggacgcggc ttccatgcat ttgcccgttg acgggtatct ggcaatttta
     7261 ggcgcgttgc tggcaggcac cgcgacatta agtccttttg cgacggcggc agcgttacga
     7321 atcagcattc aataacgctg gattactgat ttttgttcga gtctggtatc gaaactatgt
     7381 ggaaaacact gcatcaactg gcgatcccac cacggctgta tcaaatctgt ggctggttta
     7441 taccgtggct ggcaattgcc agtgtggtcg tgcttaccgt cggctggatc tggggattcg
     7501 gctttgctcc ggctgattat cagcagggaa atagctaccg cattatctac ctgcatgtgc
     7561 ctgcggcgat ctggtcgatg ggcatttatg catcaatggc agtggcagcg tttattggcc
     7621 ttgtctggca gatgaaaatg gccaacctgg cggtggcggc gatggccccc attggtgccg
     7681 tgtttacctt tattgccctg gttaccggct ctgcatgggg aaaaccgatg tggggcacct
     7741 ggtgggtatg ggatgcacgt ctgacttctg aactggtgct gctttttttg tatgtgggtg
     7801 tgattgccct gtggcacgcc ttcgacgacc gccgtctggc gggccgtgcg gcaggtatcc
     7861 tggtgctgat tggcgtggtg aatctgccga ttattcatta ctccgtggag tggtggaaca
     7921 ccctgcatca gggatcaacg cggatgcagc aaagtatcga tccggcgatg cgttcgccgc
     7981 tgcgctggtc gatttttggc ttcctgctcc tgtctgccac gctgacgctg atgcggatgc
     8041 gtaatttgat tttgctgatg gaaaaacgcc gtccgtgggt gagtgaactg atactgaaaa
     8101 gaggccgtaa atgacccctg catttgcttc ctggaatgaa tttttcgcaa tgggcggtta
     8161 cgcctttttt gtctggctgg cggtggtgat gaccgttatt ccgctggtgg ttttggtcgt
     8221 gcactcggtg atgcaacatc gcgcaattct gcgtggtgtg gcgcaacagc gggcgcgtga
     8281 ggcgcgttta cgtgctgcgc aacagcagga ggctgcatga atattcgccg taaaaaccgc
     8341 ttgtggattg cctgtgccgt gttggcaggg ctggcgctga ctatcggtct ggtgctatat
     8401 gcgctgcgct cgaatatcga tctcttttat acgccggggg aaattctcta cggcaagcgt
     8461 gaaactcagc aaatgccgga agtcggtcag cgtctgcgcg ttggcgggat ggtgatgccg
     8521 ggtagtgtgc agcgcgatcc caattcgctg aaagtgacct tcactattta cgatgctgaa
     8581 ggctcagtgg atgtctctta cgaaggcatt ttgccggatc tgttccgtga agggcagggc
     8641 gttgtggtgc agggcgagct ggaaaaaggc aatcatatcc tcgcgaaaga agtgctggcg
     8701 aaacacgacg aaaactacac gccgccagaa gttgagaaag cgatggaagc taaccaccgt
     8761 cgcccggcga gtgtttataa ggacccagca tcatgatgcc ggaaattgga aacgggctgc
     8821 tgtgcctggc gttagggatt gcgctgctgc tgtccgtgta tccgctatgg ggcgtggcgc
     8881 gcggagatgc gcgcatgatg gcgtcttccc gcttgtttgc ctggctgctg tttatgtctg
     8941 tggctggcgc atttctggtg ctggtcaatg ctttcgtggt caacgacttt accgtcacct
     9001 atgttgccag caactccaat acccagcttc cggtgtggta tcgcgtggcg gctacctggg
     9061 gcgcgcatga aggctcgctc ctgctgtggg tgctgctgat gagcggctgg acctttgcgg
     9121 tggcgatttt tagtcagcgt attccgctgg atattgtggc ccgcgtactg gcgataatgg
     9181 ggatggtcag cgtcggcttt ttgctgttca ttctctttac ctctaaccca ttctcacgca
     9241 cgctgccgaa cttcccgatt gaagggcgcg atcttaaccc gctgttacag gatccggggc
     9301 tgatcttcca tccgcctctg ctttatatgg ggtacgtggg tttctcggtg gcgtttgctt
     9361 ttgccattgc ttctttgttg tgcgggcgtc tggacagcac ttatgcgcgt tttactcgtc
     9421 cgtggacgct ggcggcgtgg atcttcttga cgctcggcat tgtgctcggt tccgcatggg
     9481 cctattacga actcggctgg ggcggctggt ggttctggga tccggtagaa aacgcctcgt
     9541 ttatgccgtg gctggtgggg actgcgctga tgcactcact ggcggtcact gaacaacgcg
     9601 ccagcttcaa agcgtggaca ttactgctgg caatcagtgc cttctcgttg tgtctgctgg
     9661 ggactttcct ggtgcgttcc ggcgtgctgg tatcggtaca cgcgtttgcc tctgatccgg
     9721 cacgcggtat gtttatcctc gcctttatgg tactggtgat tggtggttcg ctgctgctgt
     9781 ttgccgcgcg tggacacaaa gttcgctcac gcgtaaacaa tgcgctgtgg tcgcgggaat
     9841 ctctgctgtt agcgaacaac gttttgctgg tcgccgcgat gctggtggta ttactgggga
     9901 cgctgctgcc gctggtgcac aagcaactgg gactgggcag tatttcgatt ggcgaaccgt
     9961 tcttcaacac catgtttacc tggctgatgg tgccgtttgc gctgctgctt ggtgtcggtc
    10021 ctctggtgcg ctgggggcgc gatcgcccac gtaaaatccg caatttattg attatcgcct
    10081 tcatctcaac gctggtgctg tcgctgctgc tgccgtggct gttcgaaagc aaagttgtgg
    10141 cgatgacggt gctcggcctg gcaatggcct gctggattgc tgtgctggca attgcggaag
    10201 ctgcgctgcg tatttcacgt ggcacgaaaa ccaccttcag ttattgggga atggtggcgg
    10261 ctcacctggg gctggcagtg acaattgttg gtattgcctt tagccagaac tatagcgttg
    10321 agcgtgatgt gcgcatgaag tccggcgata gcgtcgatat tcatgaatat cgcttcacct
    10381 tccgtgatgt caaagaggtg actggcccga actggcgtgg cggtgtggcg actatcggcg
    10441 taacgcgcga tggcaagccg gaaacggtgc tgtatgcgga aaaacgttat tacaacactg
    10501 ccgggtcgat gatgaccgaa gcggcaattg acggcggcat cacgcgtgac ctgtacgccg
    10561 cgctcggtga agagctggaa aacggcgcgt gggccgtgcg tctttactac aaaccatttg
    10621 ttcgctggat ttgggcgggc gggctgatga tggcgttggg cggactgctg tgtctgtttg
    10681 atcctcgcta tcgtaagcgc gtgagtccgc aaaaaactgc gccggaggcc gtatgaagcg
    10741 caaagtattg ttaattccgt tgattatctt cctggcgatt gccgcggcgc tgctgtggca
    10801 gctggcgcgt aatgccgaag gggatgatcc gaccaatctg gaatcggcgc tcattggcaa
    10861 gcctgtgccg aagtttcgtc tcgaatcact ggacaatccg gggcagtttt atcaggcgga
    10921 tgtgctgact cagggcaaac cagtgctgct taacgtctgg gcgacctggt gtccgacctg
    10981 ccgtgcggaa catcaatatc tgaatcagct ttcagcgcag ggcattcgcg tggtcggcat
    11041 gaactataaa gacgatcgcc agaaggcaat cagctggctg aaagagctgg gcaatcctta
    11101 cgcgctaagc ctgtttgatg gcgacggcat gttagggctg gatctcggtg tctatggcgc
    11161 gccagaaacc ttccttattg acggcaacgg tatcattcgc tatcgccatg cgggcgatct
    11221 caatcctcgc gtctgggaag aagagatcaa gccgctgtgg gagaaataca gtaaggaggc
    11281 cgcacaatga ggtttttatt gggcgtgctg atgctgatga tctccggctc agcactggcg
    11341 accatcgacg tgttgcagtt taaagatgaa gcacaggaac aacagttccg tcagctcact
    11401 gaagaactgc gctgcccgaa atgccagaac aacagcattg ccgattccaa ctcgatgatt
    11461 gccaccgacc tgcgtcagaa agtgtatgaa ctgatgcagg aaggtaaaag taaaaaagag
    11521 attgtcgatt atatggtggc gcgttacggc aacttcgtca cttacgatcc gccgttaacg
    11581 ccgctgaccg tgctgctgtg ggtgcttccg gtagtggcta ttggcattgg cggttgggtc
    11641 atatacgccc gctcgcggcg tcgggtacgc gtagtgccgg aagcgtttcc tgaacaaagc
    11701 gtgcaggaag gtaagcgtgc cggatatatt gtttatctgc cgggtattgt ggtggcgtta
    11761 attgtggctg gcgtcagcta ctaccagact ggcaattatc agcaggtgaa aatctggcag
    11821 caggccacgg cacaggctcc ggcgttgctg gacagggcgc tggatccgaa agccgatccg
    11881 ctcaacgaag aagagatgtc gcgtcttgcg ctggggatgc gtactcaact gcaaaaaaat
    11941 ccgggagata tagaaggctg gattatgttg ggccgcgttg gcatggcgct gggtaacgct
    12001 agtatcgcca ccgatgcata cgctactgcg tatcgcctcg atccgaaaaa cagcgatgcc
    12061 gcactgggtt atgctgaagc gttgacacgt tcatctgatc ccaacgacaa ccgcctcggt
    12121 ggtgaactgc tgcgccagtt ggtgagaagc gaccacagca atatccgtgt gttaagcatg
    12181 tatgcgttta atgcctttga gcagcagcga tttggcgaag ccgttgccgc gtgggagatg
    12241 atgttgaaac tcttacctgc caacgatact cgccgtgcgg tgattgaacg tagtatcgcg
    12301 caggcgatgc aacatttgtc gccgcaggag agtaaataaa aagaggcccg atggtgatgc
    12361 catcgggcta tttttattgt gccccgcgtt gttgcaggaa cagaatggtg gctgccacgc
    12421 gtgagcggac attgagtttg cgcagcagat tgcgaatatg tacttttact gtctgctctg
    12481 aaatattcaa caccgaggca atctgtttat ttgacagccc ctgtgccagc tcgtgcagaa
    12541 catccagctc gcgctccgtc agcacgctga agggatcttc ttccgcgcca aacatttcac
    12601 gttcacgtaa gtactgattg acgcgttcgc taaagacttt gctgcctttc gctccagcac
    12661 gaattgcttc cagcaatact tccgggtcgc tgtctttcaa cagataaccg tctgcgcctg
    12721 cgtctatcag tgcaaagaca tcgctggagg catctgatac ggtcaggata ataatttgcg
    12781 cggtaacgcc atccctgcgc aaggcattga gggtatccag gccactcata cctttcatat
    12841 tgagatccag caagatcacg tcgatatcca gtctattcgc cagatcgatc gcgctcgcgc
    12901 cgtcgcccgc ttcggcgacc acttcaaagc caggatcaag ctccagtaac tgacgaacac
    12961 cgcgtcgcat aagtggatga tcatccacaa tcatcacctg aaaaggcgtt gcttcaggca
    13021 tagtagtctc ctgaggtttt attagaatca ttatgtttga gcgtagcgtt ggtaacgcta
    13081 ccaggcgcgt gaatgttacc ccattttatg tatggacatg tagtgaaaac tacaaccttg
    13141 tcgttcgctt tggcatcatt ttcattataa agacggaatt ttgcatcagc ttctcatcaa
    13201 gaaaaacaac taaaaaatca atcacaatac ttgtgcattt ctttaaacag tttttaaaaa
    13261 ctgactccgg gtatggagct atgggtattt tccgtaccca atgcttttaa cagcaattaa
    13321 tttcatagga tgaaagctca attcatcaat ctggttctgt ttctctttgt cgttccgcaa
    13381 tatctgttct gatggctaca gcgttatatt cacccatagc attggcatca acagttgagt
    13441 atggtgagac agttgatggt gttgtcctgg aaaaagatat ccagctggtt tatgggaccg
    13501 ccaataatac gaaaatcaat cctggcggag aacagcatat aaaagaattt ggtgtaagta
    13561 gtaatactga aattaaagga gggtatcagt acattgaaat gaatggtacc gcagaatact
    13621 cagtattaaa tgacggttat caaattgttc aaatgggtgg cgcggcaaac cagactaccc
    13681 tcaataatgg tgtgctacag gtttatggcg cagcgaatga tcccacgatt aaaggcgggc
    13741 gcttaatcgt tgaaaaagat gggattaccg tccttgccgc tatcgaaaag ggaggattac
    13801 tggaggttaa agagggggga ttagcgattg cggtagatca gaaagcaggc ggtgctatta
    13861 aagcaagcac gcgggccatg gaggtattcg gaacaaaccg tctcggtcag ttcgatatca
    13921 agaatggtat tgctaataat atgttgttgg aaaacggcgg aagtttgcga gttgaagaaa
    13981 atgacttcgc ttataatacc actgtagata gtggcggctt actggaggtt atggatggcg
    14041 ggactgtaac tggcgttgat aaaaaagcag gcggaaaatt aattgtctca acgaatgcgc
    14101 tggaagtgag tggtccaaac agtaaaggcc aatttagtat aaaagatggt gtgtcaaaaa
    14161 attatgaact ggatgatggt tccgggctca ttgttatgga ggacacgcag gccattgata
    14221 ctatccttga tgagcatgcc actatgcaat cgctgggaaa ggatactggt acgaaagtgc
    14281 aggcaaatgc ggtatatgat ctcggtcgat cagatcagaa tggaagtatc acgtattcct
    14341 caaaagccat ctctgaaaat atggttatca acaatggccg cgctaacgtc tgggctggca
    14401 caatggttaa cgtttcagtc agagggaatg atggcattct tgaggtcatg aagccgcaaa
    14461 taaattatgc acccgcaatg ttggtgggta aggtagtggt ttctgagggc gcttctttta
    14521 gaacgcatgg tgccgtggat accagcaaag cggacgtttc gctcgaaaat agcgtatgga
    14581 ccatcattgc cgatatcact acgacgaacc aaaacaccct cctcaactta gccaaccttg
    14641 cgatgtctga cgcaaatgtg attatgatgg atgagccagt gactcgttca tcagtgacgg
    14701 caagtgcgga aaatttcatt acgttgacca ccaataccct gtcgggaaac ggcaattttt
    14761 atatgcgtac cgatatggct aatcatcaga gcgatcagct caacgtcacc ggtcaggcaa
    14821 caggtgattt caaaatattc gtgacggaca ccggtgccag cccggcagca ggagatagcc
    14881 ttacactggt aacaacgggc ggcggtgatg ctgcatttac gttgggcaat gccggaggcg
    14941 ttgttgatat cggtacgtat gaatatacct tgctggataa tggcaaccat agctggagtt
    15001 tggcagagaa tcgcgcgcaa attacccctt caaccactga tgtgctgaat atggcggcag
    15061 cacaaccgct ggtatttgat gcagaactgg acaccgtgcg tgagcgtctt ggtagcgtaa
    15121 aaggcgttaa ttacgatacg gcgatgtgga gttcggcaat taacacccgc aacaacgtga
    15181 ccactgatgc gggcgctggc tttgagcaaa cattgaccgg gctgacgctc ggtatcgata
    15241 gccgtttctc ccgtgaagaa accagcacaa ttcgcggctt gttctttggt tactctcatt
    15301 ctgatattgg ttttgatcgc ggcggcaaag gcaatgtcga tagctatacc ctgggggctt
    15361 atgccggttg ggagcatcag aacggtgcct atgttgatgg agtggtgaaa gttgaccgtt
    15421 ttgccaacac catccattgc aagatgagta atggggcaac agcgtttggc gattacaata
    15481 gtaacggcgc gggtgctcat gtcgagagcg ggttccgttg ggttgacgga ttgtggagtg
    15541 ttagacccta tctggccttt accggcttta ccacagatgg tcaggactac acgttatcaa
    15601 acggcatgcg cgcggatgtg ggaaataccc ggatattacg cgctgaagcg ggaacggcgg
    15661 taagctatca catggacctg caaaacggta cgacgctgga accctgactg aaagccgccg
    15721 tgcgtcagga atacgccgat tctaaccagg tgaaagttaa tgacgatggc aaatttaata
    15781 atgatgtggc tggaacccgt ggcgtttatc aggctgggat aaggtcatcg tttaccccga
    15841 cgttaagcgg tcatttgtca gtcagctatg gcaatggcgc aggggtagaa tcgccgtgga
    15901 atactcaggc gggtgtggtc tggacgttct gataacaaaa aataaacagg ctgtgatgtg
    15961 tcacggtctg tttatcgaat taattgcaga tataaaaaaa accaaccgta aaggttggtt
    16021 ttttcttggg atttttggtc ggcacgagag gatttgaacc tccgaccccc gacaccccat
    16081 gacggtgcgc taccaggctg cgctacgtgc cgactcgtgg ctgctaatac taccgttttc
    16141 cacaccgatt gcaagtaaga tattttgcta actgatttat aattaatcag ttagcgataa
    16201 aacgcttctc gtctgtcagc acttgcaata acaaacttaa ctgtggtttt tcatctttca
    16261 ctctttcacc acgtaagttg taagtgcggt atttaccgtt attgttcagc accagcgtct
    16321 ttttcggggt ggtaattgcc agcgtatcgt tatccgctgc agtaacccag taatgacggc
    16381 gttgagggtt gaacaaatcc tgaccttgcg aatattcgct ggcaggtgtg ctgacatgta
    16441 gcaggcgttg catcagcgtc gtcatcagat cggtatgatc agtcagagca ttaatacgct
    16501 gcgccggcgt gcctggccag tgaatcacta atggcacctg cagatgaccg tgggaccagt
    16561 caaaggtttc ttcgtcttcg ctcagcggaa taccccgacc ggcagtgata atcaccaccg
    16621 tattgtccag tttgccagaa tcacgcagtg cattgagcac gcggttgatc tggtcatcga
    16681 cattgcctgc cgcccggcta tatttccgtg caaatgcctg ctgattgctg tcgtcaatgt
    16741 tagtgccatt gaaagagacc cacgagaacc agcggttatc ttcttgtgcg tagcggccca
    16801 gccagttgat ccactgcgtg gcggtctgct cgtcggattg ggtgcgtacg ctcggcatcg
    16861 agaaatctga caacaatgcc tggcgataca gcgggctggt aaagccatct gatgagaata
    16921 accccagctg atagccttgc tgattaagcg cagtaattaa tgccgcaggc gtacgggtcg
    16981 acagaatgcc gtccatatag ctcggcgaga tgccatagaa caggccaaag atgccgttgt
    17041 ctgtagtgtt gccggagctc atatggcgcg tgaacgaaat attttgctca gcaaaacctg
    17101 ccagcgcagg catctgcttc tcgaagcgtg agtagttcag gccatcgaca gtaatcaaca
    17161 gcacattctg cccggtgccc atatcgcgat agcgcagttc gcttaacgga tactgaacgg
    17221 aaacggcgtc tggattacct tgctcaataa gacggcgttg atactcctgc gcatcaagca
    17281 gaccatgctt ctcaagaaaa cgtcgcgccg tcatcgggta cgaaagcggc aggttagcgc
    17341 gctgcatggt gatcgggcga tagaagttgg catcggccca gatatacacc acatgcgagg
    17401 cgataaaggc gataaataag aatgcggcca gcgggcgcgc gaagcgtcga cgacgcgtca
    17461 ggctgcgcag cttttgccag ctccacgtcg caaacaccag ttcaagcaat aaaataaccg
    17521 gcacgctgat gaacatcagc tgccagtcgc gcgccatctc attttcgtct gggttgataa
    17581 ccagttgcca gacgatggga ttaagatgga gatggaaacg agtaaagact tcgctatcga
    17641 tcagtaatag cgtcattccc gccgttgcca gaatgacgga caaaaacctc atcagcctct
    17701 gggagccgac gataaaggtc agcgggaaga ggatcagcaa gtaggtggcg aacaccagga
    17761 agctgaaatg gccgataatg cttacgtagg aataaatgcg accagcaagc gttgtcggcc
    17821 agtcggcgat aaacaggtaa cggctgccaa tgacgagcga aagcagaata ttgaacagtg
    17881 caaaccagtg cccccaactg accatctggg agactttttc acggtagcgc tgacgatgag
    17941 ttaccataac ctgttatctg tttcccttag tgcgctttgt cttcgttgat agaggactgt
    18001 aaggcgcggg caaaagagtt ggcaattgcc tggcgttggg ccggggcaat gctggtgttg
    18061 ataaggttag tgaccatgtt gcctaacacc atcagggaaa ggtcggtcgg agccttatgt
    18121 ttttccagta cgttgagcag ctctgcgagc aattgttcaa cctgttcatc actgtagcgg
    18181 gaaatttgtg gcataaatcg aaatcagtta gttgatgaaa gggcaacata ttaccgtagc
    18241 aacggttttt tttctgcttt tttatgcgtt aaatcattct cgttacgctg ccttctgtaa
    18301 aatgaccgcc atcttttatt gcgtcgctta accggcggtg gttgaatacc gcccggtctt
    18361 aaaggagagt ttatcatgag tctggatatc aaccagattg ctctgcacca gcttatcaag
    18421 cgtgatgagc agaaccttga gctggtgttg cgcgattcat tgctggaacc gacagaaacc
    18481 gtcgttgaga tggtggcaga actgcatcgg gtctatagcg ccaaaaataa agcttacggc
    18541 ttgtttagcg aagagagtga actggcgcag acgctgcgct tacagcgtca gggcgaagag
    18601 gactttctgg catttagccg cgcggcaacc gggcgcctgc gtgacgaatt agcgaaatat
    18661 ccttttgctg acggtggatt cgtgctgttt tgccactacc gttatctggc ggtggagtat
    18721 ctgctggtgg cagttctgag caacctgagc agtatgcgcg ttaacgaaaa tctcgacatc
    18781 aatccaaccc attatcttga tatcaatcat gcggatattg ttgcgcgtat cgatttaacc
    18841 gaatgggaaa ccaatccaga gtccacccgc tatctcactt tcctgaaagg gcgagtaggg
    18901 cgcaaagtgg cggatttctt tatggatttc ctcggggcca gcgaagggtt aaacgcgaaa
    18961 gcgcaaaacc gtgggctgtt gcaagcggtt gatgatttca ctgccgaagc acaactggat
    19021 aaagcagagc gacagaacgt gcgccagcag gtttatagct actgtaatga gcagctgcag
    19081 gcaggcgaag agatcgaact ggagtcgctg tcgaaagagc tggcaggcgt cagcgaagtg
    19141 agtttcacgg agtttgcggc tgaaaaaggc tacgaactgg aagagagctt cccggcagac
    19201 cgtagcacac tgcgtcagtt gacgaagttt gccggtagcg gcggtggctt gactattaac
    19261 tttgatgcga tgctgttagg tgaacgcatc ttctgggatc cggcaaccga taccctgacc
    19321 attaaaggca cgccgccgaa tttgcgtgac caattgcagc gccggacgtc tggcggcaat
    19381 taatcggttt tgtgttctga atgccagatg cggcgtgaac gccttatctg gcctgcaaaa
    19441 aatagcaata ccgtaatcag gcataatctg cagatacaaa aaaccccgcc ggagcggggt
    19501 tttttacaac ttattcagca attaagcgcg aacgaagtcg atgtgctgca gcttcggttt
    19561 gtacgggtga cgctgtacgt cctgagcttt aactttgatt tctttaccgt caacaacgat
    19621 ggtcagaact tcgctgtaga attcagcttt agcttgcatg ttcatgactt tgtcgtgatc
    19681 cagctcgata gccagcggcg cttctttgcc accgtagatg attgccggga acttgttagc
    19741 ggcacgcagg cggcggctcg cacccttacc ctgctcttta cgtacttctg cgttgatagt
    19801 aaacatttct ttctctctta aattaatcct gctacaggcg acccagcaac aggtaagtga
    19861 tctgctttgc gtatgcaaaa gcgggcgaga ttctatactc aatcgcttta tacatcaatc
    19921 aaaactacca ttaaccgcgt aattcgtgcg cccgacgaaa acgaccttcg taatcgaaca
    19981 ctttttcacg cacttgccag tactgacctt tcatgcgggc aacgacaaaa tccgggtgtc
    20041 gcaataaggc ttgctgggcg aggatatcgg cggcggtgat ccagcgcaga gggatgcccg
    20101 gtgtgcgcgt atgcgggcgg ataaaaagct gctcgaaggc agttcgctgg gcgggcgttt
    20161 gcagacggaa acgctcactc acatccgcgc cgtcttcatc gtaataggtg attttcaacc
    20221 attcgccttt ctcgtcatgc ccatgctgta aagacatgcc gctacagcgt aataccagcg
    20281 cgtctttcag tcgtagcgcc gcttttaaca tatcgtctgg atcaaccagt acggtgtcac
    20341 attcgcggca gcggcgagcg gcaatatcgt tttccgcgtt acattgcggg caatttttaa
    20401 aacggaaacg gaagtcacat tgttcgcgat gaccgtcgtc atcttcaaac catccctgac
    20461 agcgacgacc aaagtgttca atcaatgtcc cgtcggcggt cgttttcccc caaaaggtgt
    20521 tggcaaaacc gcaggcaggg cagaaaacct gtaccggaac gttgtcactt ttgccttttg
    20581 gtgtaccaac ttccggcgcg tagagatcgt gaggattacc cgcataatca agaattaagc
    20641 aatcagtctt gcccggagcg agacgcagac cgcgcccgac aatttgttgg taaagactca
    20701 ctgattcggt agggcgcaga atggcgataa gatcgacgtg cggggcgtca aatccggtgg
    20761 tcagtactgc gacgttgacc agatagcgaa aacgctgggc tttaaaatct tcaattaaca
    20821 catcgcgctc agcgccgggg gtgtcgccag taatcagtgc agcatcttcg gcaggcagta
    20881 atcccacaat ctcttttgcg tgttcaacgg tggcggcgaa aatcattacc cctttgcgct
    20941 tttcggcgaa ctccatgatc tggctgatga tatgtggcgt aatgcgttgc tgttttttca
    21001 gctcacggtt gagatcagct tcgctgaaca gcccgttact ctgtgcctgc aagcggctga
    21061 aatcgtattg cactactggc atatccagtc gttctggcgg cgtcagatag ccgtgtttaa
    21121 tcatataacg cagcggcagc tcataaatgc agtcacggaa aagggctttc tcatcgccgc
    21181 gtaccatgcc gtgataatgg aactggtaga tccagccttt gcccaatcga aaaggcgtgg
    21241 cagtcagccc cagcaggcgt aagtggggat tcactttagt caggtgagtg aggatttgct
    21301 gatactggct ctcttcatcg tcaccaatac ggtggcattc atcgacaatc aacagcgaaa
    21361 attcaccctg aaaggcatca agattacgag cgaccgactg cacgctgcca aataccactt
    21421 taccgtggct ctctttgcgc tttaacccag cggcaaaaat atcggcttcc agccccagcg
    21481 cctgatactt tgcatggttt tgcgccacca gttctttgac gtgtgccagc accagaacac
    21541 gaccacgcgc cagccgtgcc agttcggcaa tcaccaggct tttacctgcg ccggtgggca
    21601 gcacgataac ggcaggggtt ttatgacgac gaaaatggtt gagcgtggca tccacggctt
    21661 cttgctgata tgggcggagt gtaaaaatca tggtctcgcg taggtagatt attctgctaa
    21721 tagtatgcca tggatctttt tcccttgagg gatgttatta gaccgctata ctgggcagac
    21781 aacagttcag attttcgggt caatgcccga cctccgtttt tacaggcaaa atcacacaca
    21841 tgcgacttga taaatttatc gcacagcaac tcggcgttag ccgtgctatt gccgggcgtg
    21901 aaatccgcgg caatcgtgtc accgtcgatg gcgaaattgt tcgtaatgca gcgttcaaac
    21961 tgcttcctga acatgatgtc gcttacgatg gcaacccgct ggcgcagcaa catggtccac
    22021 gttacttcat gctcaataag cctcagggct atgtttgctc cacggacgac cctgatcacc
    22081 caacggtgct ctattttctg gatgaaccgg tagcctggaa actgcacgcc gcaggacgtc
    22141 tggatatcga taccaccggt ctggtgctga tgactgatga tggtcagtgg tcgcaccgca
    22201 ttacttctcc gcgccatcat tgcgagaaga cctatctggt gacactggaa tcacctgtag
    22261 ctgacgatac ggcagagcaa tttgctaaag gcgtgcagct gcataacgaa aaagatctca
    22321 ctaagcctgc ggtgctggaa gtgattaccc caacgcaggt tcgtctgacc atcagcgaag
    22381 ggcgttatca tcaggtgaaa cgcatgttcg ccgccgtggg taaccacgtg gttgagctgc
    22441 atcgtgaacg tattggcggt attacgctgg atgctgattt agcccccggt gaatatcgtc
    22501 cgttaactga agaagaaatt gccagcgtcg tctaactttt ccatgacttt caggagcccg
    22561 ttgtgaccac ccgacagcat tcgtcgtttg ctattgtttt tatccttggc ctgctggcca
    22621 tgttgatgcc gctgtcgatt gatatgtatc tgcccgcgct accggtaatt tcagcgcagt
    22681 ttggcgtacc tgcgggcagt acgcagatga ccctcagtac ttatattctg ggctttgcgt
    22741 tggggcagtt aatctacggg ccgatggcag acagcttcgg gcgtaagccg gtggtgctcg
    22801 gcggtacgct ggtgtttgcc gccgccgcgg tggcgtgtgc gttggcaaac accatcgatc
    22861 agctgattgt gatgcgtttc ttccacgggc tggcggcggc tgcggccagc gtggttatca
    22921 acgctttgat gcgcgatatt tacccgaaag aagagttctc gcggatgatg tcgtttgtca
    22981 tgctggtgac cactattgca ccgttgatgg caccgattgt cggcggttgg gtgctggtgt
    23041 ggctgagctg gcattacatc ttctggatcc tggcattagc ggcgattctg gcttcggcaa
    23101 tgattttctt cctgattaaa gaaaccttac caccggagcg tcgtcagcca tttcacattc
    23161 gtaccactat tggtaacttt gcggcgctgt tccgtcataa gcgtgtcctg agttatatgc
    23221 tggccagcgg tttcagcttt gccggaatgt tctcgttctt aagcgccgga ccgtttgtct
    23281 acattgaaat taaccacgtc gcgccggaaa actttggtta ttactttgca ctaaacattg
    23341 ttttcctgtt cgtgatgacc atttttaaca gccgcttcgt tcgccgcatt ggcgcgttaa
    23401 atatgttccg ctcggggttg tggatacaat ttattatggc agcgtggatg gtcatcagtg
    23461 cgctgctggg gctgggattc tggtcgctgg tggttggcgt tgcggcgttt gtgggctgcg
    23521 tgtcgatggt gtcatccaat gcgatggcgg tcattcttga tgagtttccc catatggcgg
    23581 gaacggcatc ttcgctggca ggaaccttcc gttttggcat aggggcaatt gttggcgcat
    23641 tgctttctct tgcgaccttt aactctgcat ggccgatgat ttggtcaatt gcattctgcg
    23701 caagcagctc cattctcttc tgtctgtacg ccagtcgacc gaaaaaacgg tgatctattg
    23761 cacaacgagg aagctaaaag gcttcctttg ttgatgcatg tcaaccacaa atctatcatt
    23821 cccccgatat atgtttattt tatgtaaaat caatttatgt aaaaagtcac atcattgtag
    23881 ttaaaaaggt tgagttagat cgcagaaacg ggtacatata gccccgcaaa cgtgaccacg
    23941 cccgcagata ttacttaaat cagagccata gaggccacgc aggcgaggca tcaatcttta
    24001 cgatctgtat aaagacggat tgttgatgat gtgttaaaat tgatgtaaac aaattgtgaa
    24061 gtgaatgtac ttccggggaa aataagtgac ttcattacaa ctctcaatcg tccatcgact
    24121 gccgcagaac tatcgctggt ctgctggttt cgcaggttcg aaggttgaac cgattccgca
    24181 aaatggaccg tgcggtgaca acagcctggt ggcgcttaaa ttgcttagcc cggatggtga
    24241 taatgcatgg tcggtgatgt ataaactaag ccaggcgtta agcgacatcg aagttccatg
    24301 ttcggtgctg gagtgtgaag gtgagccgtg cctgtttgta aatcgccagg acgagtttgc
    24361 tgcaacatgc cgattgaaaa attttggtgt ggcaattgcc gaaccctttt caaactacaa
    24421 tcctttttaa gcgtcagctc aacgccagta gctgacgcgt atactcctgc tgcggtgtgg
    24481 caaacacgcg cgcgcacggt ccttgttcca ctacttcccc ttgtcgcagt acgataacct
    24541 gatgacataa cgcgcggaca acgtgcaaat cgtggctgat aaacaaatag gccagttgat
    24601 gcttttgttg caatgatttc aatagcgtca atatttgcgc ctgtaccgtt ttgtcgagtg
    24661 atgatgtcgg ttcatcaagt atgatcagcg agggcttaag aattaatgcc ctggcaatcg
    24721 caatacgttg tcgctgacca ccagagaact ccgccggata acggtggcgt gtttcaggat
    24781 ctaatcccac ttcatgcatc acggctatca cttgttgttc gcgttgtgcg gcagaaagcg
    24841 tcggctggtg aacccgtaag ccttcctcaa taatctgcaa aacgttgagt cgtgggttga
    24901 gcgaggagtt tggatcctga aataccacct gaatgcgatg acgaataggt aacagctggc
    24961 ggcgatttaa attttgcagt ggctgaccgt caaagatgat gctgccctga gaattaatca
    25021 gtcgcagcag cgccagtccc gtcgtacttt tcccggaacc ggactcgccc actaaaccca
    25081 gtgtttcacc cgctcgtagc gtaaaactga tgtttttcac caccacatta tgatccacaa
    25141 tgcgcttcaa aatccctttg cgaatgggga aggcaacctg aagctgttca acatccagca
    25201 acgttgaggc aggttctggc aacggcactg gatcgcctga cggttcactg ttgagtagct
    25261 tttgtgtgta aggatgagtg ggtgatgcaa atagcgtagc ggcgtaattt tgctcgacac
    25321 agcgaccgtt ttgcattacc gccacgcggt gggccagttt tctgacaatg ctgaggttat
    25381 gagtaataaa cagcatgccc atattcagct cgccttgcag ttcgcgcaac agctgtaaaa
    25441 tctgcgcctg gacagagacg tccagtgcgg tggtcggttc atcggcaatt aataattccg
    25501 gtcgcgttaa cagcgccatc gcaatcatca cccgctggcg ttcgccgccg gagagctgat
    25561 gcggataatc tgtcagccgt tttgccgcct ggcggatacc aacgcgatca aggcagttaa
    25621 gaatttcgcc acgagccgct tcccgacgca tcccgcggtg gagtgaaagc acttcataaa
    25681 gctgtttttc cagggtatgc aatggattta acgacaccat cggttcctga aaaatcatgg
    25741 cgatcttatt accgcgtaca ccgcgcaacg tttgatcgct gacatgaagc agcgattcgc
    25801 catgaaaacg aatatcgccg gagagatatt caaccggcgg ggaagggagc aggcgtaaaa
    25861 ttgacagcgc ggtaacgctt ttgcctgaac ctgactcacc caccagcgcc agcgtttcgc
    25921 cagcctcaat ctgtagtgaa acatcattga ctactgtacg tacggtttgc tgatggcgaa
    25981 aacccaccga caaattttca atcgctaaca gagtttgcgt catgctacac cgccttatta
    26041 ggatcaaatg cgtcgcggac ggcttcacca ataaagatca gcaaagacaa taatatcgcc
    26101 accgacaaga aggcggtgat cccaagccac ggggcctgaa ggttattttt cccttgtaac
    26161 agcagttcac cgagtgacgg tgaaccgagc ggtagaccga agccgaggaa atcgagcgag
    26221 gtcagggtgg ttatcgaact acataaaata aacggtaaaa aggtgagggt cgcgaccatg
    26281 gcattaggca acatatgacg caggatgata ctgcgatcgc tgacgccaag cgcctgtgcc
    26341 gcacgaatgt agtcgaaatt acgagtacgt aaaaactccg cccgcaccac gccgaccaga
    26401 ctcatccagc caaacaagac agtaattgcc agcagccacc agaagttagg ctgtacgacg
    26461 ctggaaagta aaataatcaa aaacagcgtc ggcatccccg accatacttc aataaagcgt
    26521 tgtccccaga gatcgacttt accgccgtaa tagccttgta gcgcccccgc cagcacgccc
    26581 atcacgctgg aacagagagt cagcatcagg ccaaacagaa ccgagatccg cgtgccatag
    26641 agaatgcgtg ccagcacatc gccgccgttg gcatccgttc ccagccagtt ttgccgggag
    26701 gggggagaag ggaagggctt atcggtagca aagttgatac tggtagcacc aaagcgaatc
    26761 ggtgcccaca gtacccagcc gttattttcc agccgttgtt tcagccacgg gtcctgataa
    26821 tcagcctgac ttgccagcgg gccgccaaaa tcgctttcgc tgtagttttt caacaacggg
    26881 aaataccaac tgccgtcata acgtaccagc aacggtttat cgttggcgat aagttcagaa
    26941 cacaaactca aaccaaacaa aacgaggaaa atccataacg accagtagcc gcgacggtta
    27001 tggcgaaaac gcgcccaacg ggcctgattg acggggctga gtcgcgacat taacgtccct
    27061 caaaatctat acgcggatcg accagcgtat agctgatatc actgacaata ttcagcagca
    27121 ggccaatcag ggtgaaaata tacaaggtac caaacattac aggataatcg cgcgagacgg
    27181 tcgcttcgta gcccagtaag ccaaggccat tgagtgaaaa catcacttca atcagcagcg
    27241 agccggtaaa aaacatgctg ataaacgtcg ccggaaaacc ggcaatcacc agcagcatgg
    27301 cgttgcggaa cacatgtttc cagagaatat ttttttcact tacccctttc gcacgcgcgg
    27361 tcaccacgta ttgcttgcgc acttcatcaa ggaatgagtt tttggtcagc atggtcagcg
    27421 ccgcaaagcc accaatcact gtcgccagca ccggcagcgt gatatgccac agataatcgg
    27481 tgattttctg ataccacggc agcgaatcaa agttagcgga aaccaggccg cgtagaggga
    27541 acaggtcgaa atagctgccg cccgcgaaga agacaatcag caggatggca aacaaaaagg
    27601 ccggaatggc gtagccgatg atgataaatg cgctactcca gacgtcaaag cggctcccat
    27661 tataaacagc tttgcgaatg cctaacggaa tcgacaccag atagataatc agcgtgctcc
    27721 acaatccgag ggtgatggaa accggcagac tgtctttaat cagcgtcagc accgaggcgc
    27781 tgcgaaaaag gctatcacca aaatcaaagc ggatgtagtc ccagatcatt ttgaagtaac
    27841 gttcgtggat cggcttatcg aaaccgtagc gatgagtgat ctcggcgatc acttctggat
    27901 ctaatccgcg tccgccacgg taattactgt cgctgatatt gccgacgccc gtttgcgcat
    27961 ggctggcgcg aataccttca ccgcctgcgc cgggtaatac tccggcatta ccaaactcaa
    28021 tggcggcgat ggcctggtcg accggaccgc caggcgcaat ttgcacgatg aaaaagttga
    28081 tagtgataat cgcccataac gttgggatca ccagcaacag acggcgaatc agataagcgc
    28141 ccatctactc tccctgttgt ctggctgacg gcagtttggc cgctttatta acgtcatacc
    28201 accaggtatc gatacccagg ctgtaaacag ggcgtacagc gggttgggag aatttatccc
    28261 accaggcgag acggtcttcc gccatgtacc acattggcag catgtaataa ttccacgtta
    28321 atacgcgatc cagtgctcgc cctaacggca gtaatttttc tttatttccc tgcgcggcaa
    28381 taatttgatt gatcagcgag tcgataaccg gactttgcac gccgggggca ttataagtgg
    28441 aattgatata ttccgatgac caggaaatct gtaaatcgga actgggccac ggcatcgccc
    28501 gccatacgcg cggcatcatg tcatagtcgc gactgcgcat gcggttggtg atttgcgagt
    28561 tatccacctt gcgaatgtcc atgttgatac ccagccgttg caggctgtgc tggaacggca
    28621 atacccactg actattgctg cttgcgggaa gcaataattc aaagctgagt ggctgacccg
    28681 ttgtggcatt aacgcgttgc tgacccttca gcacccagcc cgcttcgttg agaagtttgt
    28741 cggcttttaa caggttgtca cgatcgtagc catcgccttt agataccggc ggctggtaga
    28801 tttgtgtgaa gacttctgac ggtagatctt ttttcattgg tgccagaagc accagctccg
    28861 cggcgtcggg gtaatttctg gccgcgtatt cggtattctg aaaataactg ttcgtgcgac
    28921 tccaggcatt gtaaaacaac gccttgttca tccattcaaa gtcaaaggcg agggtgattg
    28981 cttcccggac ccggcgatcg ctgaataccg gacgttggat attaaacgcc agccagcgcg
    29041 tatcctgggc tgattcgttc ttttgctcat ctttgatgat gtatttttta tcgaaatttt
    29101 taccggtata acgcgtggcc cagtttttag cgtcgttttc catacgcaaa tcaaaggcac
    29161 ctgctttaaa cgcttcaaag gcgacattat catcgaggta ataatcgtag cgaatggtgt
    29221 cgaaattcca gcgtccacgg tttaccggta agtttgctgc ccagtaatct ttcacgcggg
    29281 aatagacaat attttgcccc attttccagg acgtaattcg gtacggacca ctggcaagcg
    29341 gaggcgtggc gagcggatcg ctaagtttgt gatccttcca gtacttttct ggaaataccg
    29401 gcagcgaaaa cagactcaac atatcttctt tgccgggttt agctaactca atgcgcacgg
    29461 ttaacggcgc aatggctttg acggtggtgc ctttgtagac cagacgaaat tgcggcacgc
    29521 cttcggtcat aaatttttga aaagtaaact ctacatcgcg ggcagtaatg ggcgaaccat
    29581 catgaaaacg ggcgcgcgga ttaatagcga cctccaccca ggaataatcg tcagcatagc
    29641 gtgcgctttc agcaatcagc gggtaataac tgcctggttc gtcatcggaa gtcgtaaata
    29701 gcgtgtcgta cagctgctcg gtgcgtgcgc ccgggttgcc gcgcagtgca tagcggttga
    29761 aattatcgaa ggtaccgagg gctgacaacg ttatctgccc accttttggc gcggcggggt
    29821 tcacataatc aaaatggttg aaattaaacg cgtaccgggg ttcgcccagc acggcaaagg
    29881 catagctttc cttgatagcc tgcgcctgcg caccaaaggt gaacagagcg ataaacagca
    29941 gcagtatgcg cacaatcatc tgcaacggat ttccttttac tcatcattag ccgcaatccg
    30001 gcatgctttc actatgaata ataagggacg taagcacacc ttaccactgc ggcgtatacg
    30061 gtttcttcag ccagcgaaca aaatcgtcca gcggtaacgg gcgactaatc cagtagcctt
    30121 gcatgaaatt aacgccgcgt tcgcttagcc atcgcgcttg ttccggcgtt tcgacccctt
    30181 cagcaaccgt cagcatattg aggcgtttcg ccagcgtcag caccgcgtca agtacgggtg
    30241 aagtgatcgt ttccgtaccg atggcgttga taaatccacg gtcaattttc agataatcga
    30301 gcgtaaaacg ctcaagatag ataagcgcgc tgtgcccggt gccgaagtca tcaatagcaa
    30361 tttctacgcc taccgagtgc agccaggcga agagttgtgt ggcttcttgc tctttcagca
    30421 tatcgcgctc ggtaatttcc agcacaatct ggaaatggtg tgcgggtagg gaagtgagca
    30481 gtttctggat atctgcttta aagctttcgc tgtgcagatg gtccggcgca atgttaatgc
    30541 caaatttgac gcctaccggc agcacttttt ctaattctgc ggcatcgcgg gcaattaact
    30601 caaacagatg ctgagtcagc ggcacaatca tcttttgcga ttcggcaaag ttaatgaagg
    30661 catccggggg aatttctccc gcgacaggat gccgccagcg tagcagtact tccaggcccg
    30721 ttactcgcaa agcttgtgta tccaccaccg gttgatacgc cacgtaaaat tgttcgcgct
    30781 tgatggcggt catgatttct ctgccggggc gcatacgtac gctcatcagg taatagcaga
    30841 gcaggccaac gacagttcca ctcatgccgc ccagtaaaaa tgcgtaccac acatcgttcc
    30901 atgtccagtc atctgcataa agccgaacgg tcagaggaat gcccgcaatt ttagtttcac
    30961 ggactggcat gtcggttaat tcgttaacgt tcatcaaacg tgaagaaaag gtagatagcg
    31021 cagtattgcc aataatgagg gcgacgccat cgtaatcttc ctgccgtgaa ctataaaaga
    31081 gtgaaggcgt caggttgaga ttcagagcgg caaagacgcc gctatttttc agcaaagggt
    31141 tgcgatacca gatgacgatt gcgggtttgt tcggcaccat cggcgtgccg ggtaagatcg
    31201 ccatatcgac atttttatta atgtcgagcg ccggaatcaa ttcattgagt ggaatgtcca
    31261 tctcaccggt cgcagatgaa cagaatgttt ttttatcttt caccagcaca aacgtacgga
    31321 cattcatgct aaacgccgcg cgggcggtca gttcggggtt agcctgctgg caggtatcta
    31381 aggtcagcgg ctggagccgg tcagtagtgg atttcaggtc ggcaaaatag gtatcgagat
    31441 acttttgtac gtcggtaatc agtgtgtcgt atttgacttc gtgcttatgc caggccacta
    31501 aaaactgaag gcaactcacc agtatcgcaa tcatcacgcc tgcaacaatg caggtaagca
    31561 ggagcttacg tccagaattg ggagcgcgta tgaacatgat tccgttaatt tcctgaaatt
    31621 acgacaacat ttaaaagata acaatgaccg gactatagcc tggatcaaga cgcggaaaca
    31681 tcattttctc tgatgaatga aaacaaaaaa gcactgccta agcagtgctt tcagttaact
    31741 cgttaggata gccaagggat tgcatccaaa cggtttatta gctgcggctg agaacccggc
    31801 gtgcttcgtt gtaacgcttc ttccagtacg gttcattcat gctggaaata ataacaccac
    31861 tgctggtgga agcatggaca aactgattgt tgccgatata aataccgaca tggcgtcccg
    31921 ttgaaccggc acggaacaga actaaatcac ccgtacgcaa attactgcgg gaaacagatt
    31981 tacccatttc ctgctgttcg taagtcgaac gcggaagttc taagccaaat tgctcacgga
    32041 atgtacgctg tacgaaacca gaacaatcga tacctttttt agtgctgccg cccagacgat
    32101 aacgtacgcc tttccagtca gcatactgat ccataattcg cgattttacg tcgacattac
    32161 gaaccaggtt ttcaaattca tcctgagaag cttgcagtga tgatgtttca ctacccactg
    32221 cacgtgtctc aggatgcata ttctttgcgg tgttatttgc actacatgca gaaagcagaa
    32281 ccgctactgc aatcgcgggg atcccgcgca agatatatct caaaatcggt tgagatttga
    32341 ccatgttgtt tgttttccct tgaagtcctt aacgacaaat atcgttataa aaaaatgcca
    32401 agcgacacga gactaattac ctgggcatgt tgaaacaatt ccttaaggcc aaatctgtgg
    32461 actaacgcac aaaattatat gctgtgagga taatctaccg ccgaggcaaa acgagttcga
    32521 gattacccga ttggtgcggt cttggcgagc ggttttacat gattttttat aagaaaaagt
    32581 gatacagaaa gttaataagc gggattggtc aattaaagta cgattgagtg aaacgcatgc
    32641 aatccattca tttacttaga gaatgatttt tgcgcgcgat ggatgaaagc acggtgcgca
    32701 tcatcaggat tatcctcact ataaaaataa gcctgatgat gttaattact gtgagttatt
    32761 tgttttggaa atgtttgttt tttcccggta gtgatttgtc aaaaaaggtg attaatcgat
    32821 cacttaatgg cgtcagcaac acccagggca aaccgatcaa tatcacggtc atcgaaccga
    32881 caatgatgtc agtaaaccag tgtgcgccaa tcattactct gggaaatgca aaaaccacaa
    32941 aaataataag ggcgataagg cctgcaactt tgccgaaata acgccacatg aatgccgaaa
    33001 aaataagcag catcatgccg tgatcgccgg gaaagctatc tcgtgaggca tctttcgtgg
    33061 gaacagagag cagttcgctg acgcggttaa tatcggtaaa agtcaatgtt gggctggccc
    33121 gttttacagg aattaatgcc tgacccagct ggtttaatag cactgcagtt aatagcatga
    33181 ccagacccat aatcacgata cgtcgtctgc caggggcgtt ttctttcagc cagaaactca
    33241 gcatcaacat tcccatcgcc agcagagaac aaccgtcgaa agcgcgattg ttggtcaatg
    33301 caaccagcca caaaaaggcc ttactttcga ccagtttctg attaaagaaa taaaaaatat
    33361 ccgcatcaat cggcaaccag aatccatgat taacgggaat ataccaggaa agaaacagcg
    33421 cgaggccgac aatattcaac aacactattt gcggcaaatt tttaatcata ttttctctaa
    33481 ttatcttact gaaaaacgca ggcaacctta cgcggtagtc gctaaacgaa gcttcaacag
    33541 cgcgctctgc aaagcattcc agtcagcttc gctgctggaa atcagctcaa tacggctgtc
    33601 cggtggcgca acgttttgcg tttcaatgtg caggtcatcg ccctgacggt tgattcgtac
    33661 cagcccttcg ggaatacgca gcacgccttt gacgcgttcc accggtgcaa gtcgcgccca
    33721 ttccagaatg ccaatggtgt cgaataccgt atcagcgtcg aatatccagc cgcaggcctg
    33781 atatccttgc ccgctgttca gactgcgacg ccagcgttga tgctctggca ggcttaacgc
    33841 tgctaaccct tttttcacga catgctgatg agaatgcgcg gcgctggcgg gcaactcggc
    33901 taaattgcga cgcggcaaat ccagaagatg accgtcaact ttcccatgct cactgtgaat
    33961 taattgtcga tcgccaccat tttgctgcca ccaacgctgt agcgcttgct cactttcggg
    34021 cgtcgcacgg tcggatttat tggcgacaat gatgtctgcg gcagccagct ggtcacggaa
    34081 gttttcattg ctggcgcttt tttcgtccag cagcaagcgc ggatcgagaa tgcacaaggt
    34141 ggcgcgcaga tctatccacg gttcatagac tggtgcggtt aacagatcga ggatctgttt
    34201 cggatggccc agcccggtcg gctctatcaa caagcggtct ggttttccct gacgcagtaa
    34261 ggtattcaac cctacctgca tgggtaaacc attaacgcag cacatgcagc cgccggggat
    34321 ctctttcagc aatgcgccgc tatcggcgag caaagcacca tcaattccga cttccccaaa
    34381 ttcattaacc agtaccgccc atttttcgtt gggatcttta tgggctaaca gatgaagaat
    34441 cgacgtggtt ttcccgctgc cgagaaaacc ggtgatgagg ttggtcctgg tcacaacggc
    34501 tccgcaatac tctggtaagt taactgagcg ttaatcctgg ctaaaaaagg cagaaaagag
    34561 aaggggagag cggacgaagg tgagcttcat ccgctttaac cgcagaaatc gttaaatttt
    34621 gagggtgttt aatagcgcct gatgtgcgcc gaattgtacg atttgttgcc aggcttgttc
    34681 gatggcctgc acaaaatgcg ggttatctgg cagatcatca ccgaaaactt cacgcaggga
    34741 aagcagggcg gttacgcgtt gttcactgct gctgcccgca acaagttcgc gaattttatc
    34801 gctaagcgga tcgcgaacat caatggcatt tccggcatca tcaacgccgc tgacgtaacg
    34861 catccagcca gcaacgccta atgccagcaa cgaccagtcc gtttcgcacc ccagatgtat
    34921 gcgaatacct gccagcatac gttgcggtaa tttctggcta ccatccattg cgatttgcca
    34981 ggtcttatgt ttcagcgccg gattagcaaa acgtgcaatt aacttgtccg catattgtgt
    35041 taaatcgaca tctttaattt gtagtgtcgg cgcttgctca ttcagcatta atgttctggc
    35101 ggcatggcga aatgcgcgat cctgcatgca atcactgata tgggcgaatc ctgaaaggta
    35161 acccagataa gcgagaaaag agtggctgcc attaagcata cgcagtttca tctcttccca
    35221 tggcaggaca tcattcacca tttgtacacc tgcgacttcc caggcaggac gcccagcgac
    35281 gaagttatct tccaccaccc actggataaa cggttcgcag ctaatcgcgc agggatcatt
    35341 tacccccaga tgctggctga tttctgccag tgattcgtcg gttgcagccg gaacaatgcg
    35401 gtcgaccatg gttcccggaa aactgacgtg ttattttatc cacccggcga gttctggcga
    35461 acgtttttct gccattccca gcaccgcgtt tttcaccaca tggccattgt cgggaatatt
    35521 gtcgcaggag agcacggtaa acggtgtaag gcctcgctca cggcggcgtt tcagtgcttc
    35581 gacgagaata cccggtgcgg agtgaggttc ttcaggggtt tgtagatcgt gaataatccg
    35641 cggattactg gtgtcgagtg caccggtggc cgggtcaata caatagcctt tttcggtaat
    35701 cgtcagggaa acaattgcca cctgtggctc gcaaaatttc tcaataattg ccgctaagga
    35761 atccagtttt gcattaaggc attcgtgaac ggcaccgaca attatcacct gattgccgtc
    35821 cgcacctttt tccagcacgg tatataaatg gttctgtgcg cggagctggc tcatcagttg
    35881 atcaccgctg aacaagctga tttcacagat cccccagtcg ccgccctggg cattcagcac
    35941 acgatcggtc agtaacgcct gatgagcgcg gtgaaaggcg ccaaagccaa aatgaacgat
    36001 acgtgattgc aactgctggc gatcgtagcg tggagcgtgt atatgatgcg ggagcgtaac
    36061 ggaggcaatt gtcttcatag gagtcacgtc ttagtaacga taaattaaca atgacagtgt
    36121 aaagttatat gacagcaatt gaaattggtc tggattgtta atgcgatgaa gtgtgagggc
    36181 gatcaatcaa cgagtgaaaa ttgagggcgt aaggtgttca ggaagcgcaa aatagaagat
    36241 tggtataata aattaaaaaa gggacagcct gagctatccc ttttctgcat gcggctgaag
    36301 ttagtcagca cgccccatat aacggcgttc ttcgatatgg atacgaattt tttcgcccgg
    36361 gcttaagtat tctggtacct gaatcaccag accagtgctc aatgtcgccg gtttgttacg
    36421 ggcgctggca gatgcccctt tgatgcctgg tgcggtttca acgatttcca gatcaacggt
    36481 ctgcggcagc tcaagcgcca gcagttgacc gtcccaggtc agcacctgca tgtccggcat
    36541 gccgccctct ggcataaaca gcaactcttc ttcaatctga tctttggtga aggtatacgg
    36601 ggtatagtct tctttatcca taaagacata ttcgttgcca tcgacatagg agaagtcaac
    36661 gtaacggcgg gtcagcgtca cggtgtcaac gatatcatca cctttgaaac gctcttctac
    36721 tttcagcccg gtacggacat cagaaaaacg cattttgtac agcgtagcgg caccgcgggc
    36781 agtgggcgac tgaatatcaa tatccttaac cagcagcagt ttgccgttgt aattcagtac
    36841 catacctttt ttaatttcgt tcgctcttgg catcgaaaaa ttcctgtagt cgaaaatgtc
    36901 aaaaatatcg cgacaaagta ctcgcgacgg gcctttcggg caagtggtat tcgcactttt
    36961 gctggtgcaa aaaaggtggt actgtgcgcg ctcatcaatc cggtggttaa ccttaagaga
    37021 acaacgtatg aaatgccgtc cgggttgcgg ggcgtgttgc actgcccctt caatttccag
    37081 ccctattccc ggtatgccag atggcaagcc cgccaatacg ccctgcattc agcttgatga
    37141 acagcaacgc tgtaaaatat tcacctcgcc actgcgaccg aaagtctgtg caggcttaca
    37201 agccagcgct gaaatgtgcg gtaactcgcg tcagcaggcc atgacctggc ttatcgatct
    37261 ggaaatgctg accgcgcctt aaacatcttt aatccgcagt aagcaaaaca gagtggcgat
    37321 aatcatcacc atcgcaaacc agaacacagc gtgataattc cagatctcgg cgacgatgcc
    37381 cgccactgat cctgcgatga tccagcccac gcgcgacgtg ttggtataga gcgtggtggc
    37441 tgaacccgcc tgaccgggca tcagatcctg aaaatagagc atcccgatgc cgcccagaat
    37501 gccaataaaa atagcattta gcagctgcaa gcccaacaga atgacaggtg aatgcgccat
    37561 cagcattcct gcgtaaaaac agacgccacc cacggcagca acgcgcatta agaaacgctt
    37621 acccagacgt ttggcgaaat atccggcaat caacatcgtc gggatttcca gcccggcggc
    37681 ggtccccatc acaccggcca gtttctcggg aagatgcagt tcgttgataa taaatagcgg
    37741 catgttgatg atgtacaggc tgttcgagcc ccacatcaat gtacaaatga caaacagcag
    37801 cagcgtatcg cgacggttac ggcgcggcgc ttcgatcgtg ccggtcgcca gcggaagctc
    37861 tttttgcatc gacggtaaaa acagccacac catcacaccg caaacaataa acgctaccgc
    37921 tgcgctcaga tacattaccg taaagctgaa acccatcgct aaggcataag ccagcggtgg
    37981 gccaatgacc catgccagtg aaacctgagc gcgtaaaaaa gagctgaaca tcaccgcctc
    38041 acgtccggtt ttgtcggcat gttcacgggc aagggcaaac atttgcgggt tagcggtcga
    38101 gccaaagctg ctaagaaaga cgccaacgaa tagcaaaaca aagtagttgc gattccaggc
    38161 aaaaagggtg caggccagca cgcctaacag gcagcaaaag acaatcagcg atttgcgatc
    38221 gccgcgctta tcagagcgcc cggcgagaaa ctgacttacc agaatcccaa tgacagcgct
    38281 gccggtaaag aagaatccca ccatcgccgg acgagcatgt acttcatcag taagaaaaat
    38341 actgagtgtc ggggtttgca gagcgcccgc aataccggtg agaaaggcaa cgattaaaaa
    38401 cgccgtcgag gtcaggtcaa acgatttcgc gctggagact gcgggggagt tatgcatgct
    38461 ggtcgatcac tggaagagga agacgcggag tttacgctgc ttgtcagaaa ataaacagac
    38521 agcgaaatca aaagcgccac gaataatcaa aatgccattt cactgttgct gattggctgc
    38581 tgacgctgct gaaatgaaat tgctgataac ggattttccc atcagcaatt aggaaaaatg
    38641 gcaaaaaatt gtgcagcaca tcaaactttt gctcataact ttacggcttt ccttgcgtgc
    38701 tgaaacagaa gtattatgct ttcttgaaac gtttcagcgc gatcttgtct ttaaccctaa
    38761 gccaggttgg cgcttttttt ctcatagagg ctgaatcgtt tcaattcagc aagagaggag
    38821 aactatgttc cagttatccg tacaggacat ccatccgggc gaaaaggccg gagacaaaga
    38881 agaggcgatt cgccaggtcg ctgcggcgct ggtgcaggcc ggtaatgtag cagaaggcta
    38941 cgtcaatggc atgctggcgc gcgaacagca aacctcaacg ttcctcggca atggtattgc
    39001 tattccacac ggcactaccg acacccgcga tcaggtgctg aaaaccggcg ttcaggtatt
    39061 tcagttcccg gaaggcgtca cctggggtga cggtcaggta gcgtacgtgg caatcggtat
    39121 tgctgccagc tcggatgaac atctgggcct gctacgccag ctgacccacg tactgagcga
    39181 tgattccgtt gctgaacaac tgaagtcagc accaacagca gaagaacttc gcgcattact
    39241 gatgggcgaa aagcagagtg agcagctgaa gctcgacaac gaaatgctga cgctggatat
    39301 cgtcgccagc gatctgctga ctcttcaggc gctgaacgct gcgcgtctga aagaggcgag
    39361 ggcagttgac gccactttcg tcaccaaagc catcaatgaa caaccgctga acctcggaca
    39421 gggtatctgg ctgagcgata gcgccgaagg caatctgcgt agcgcgattg cggtaagccg
    39481 tgcggcaaat gcttttgatg tggacggcga aacggcagcc atgctggtga gtgtggcgat
    39541 gaatgacgat cagcccattg cggttcttaa gcgtctcgct gatttgttgc tcgacaataa
    39601 agctgaccgc ttgctgaaag cggatgcggc aacgttgctg gcgctgctga ccagcgatga
    39661 tgcgccgacc gacgacgtgt taagcgcgga gtttgtggtg cgcaatgaac acggcctgca
    39721 tgctcgtcca ggtaccatgc tggtcaatac cattaaacaa tttaacagtg atattaccgt
    39781 gacaaacctt gatggcaccg gcaaaccggc aaacggacgt agtctgatga aagttgtggc
    39841 acttggcgtt aagaaaggtc atcgcctacg ctttaccgcc cagggtgcag atgctgaaca
    39901 ggcgctgaaa gcaatcggcg acgctatcgc tgctggtctt ggggagggcg cataatgagc
    39961 agacgtgttg ctactatcac ccttaatccg gcttatgacc ttgttggttt ctgcccggaa
    40021 attgaacgcg gcgaagtgaa cctggtgaaa accaccggtc tgcatgcggc gggtaaaggc
    40081 atcaacgtgg ccaaagtatt aaaagacctg ggaattgatg tcaccgttgg cggcttcctc
    40141 ggtaaagaca atcaggatgg ttttcagcaa ctgttcagcg agctgggcat tgccaaccgt
    40201 ttccaggttg tacaggggcg cacccgaatt aacgttaagc tgacggaaaa agacggcgaa
    40261 gtgaccgact tcaacttctc gggttttgaa gtcacccccg ccgactggga acgctttgtg
    40321 actgattctc tgagctggct cggtcagttc gatatggtct gtgtcagcgg aagcttaccg
    40381 tcaggcgtca gcccggaagc gttcaccgac tggatgactc gcctgcgtag tcagtgtcct
    40441 tgcattatct ttgatagtag ccgtgaagcg ttagtagcag gtttgaaagc ggcaccgtgg
    40501 ctggtgaaac ctaaccgccg cgagctggaa atctgggcag gccgtaaact gcctgaaatg
    40561 aaagatgtga ttgaagctgc acatgcgcta cgtgaacaag gtattgcgca tgttgttatt
    40621 tcactgggtg ccgaaggcgc gctttgggtt aatgcctccg gcgaatggat cgccaaacca
    40681 ccgtcagtcg atgtcgtaag caccgttggc gcaggggatt ctatggttgg tggcctgatt
    40741 tatggcttgc tgatgcgtga atccagtgaa cacacactgc gtctggcgac tgctgttgca
    40801 gccctggcgg taagtcaaag caatgtgggt attaccgatc gtccgcagtt ggccgcaatg
    40861 atggcgcgcg tcgacttaca accttttaac tgacagcagg agaggcataa tgaaaacgct
    40921 gctgattatt gacgctaatc tcggtcaggc acgcgcctat atggcgaaga ccctgctggg
    40981 cgcggcggcg cgaaaagcaa aactggaaat catcgacaat ccgaacgacg cagaaatggc
    41041 gattgttctc ggtgattcca tcccgaatga cagcgcgctg aacggtaaaa atgtctggct
    41101 gggtgatatt tcccgggcag ttgcgcaccc tgagctgttc ctgagtgaag ccaaaggcca
    41161 tgcgaaacct tacactgcgc cggtcactgc gacagcaccg gttgccgcca gcggtccgaa
    41221 acgcgtagtt gcggtgactg cttgcccgac tggcgtagca cacaccttta tggcggctga
    41281 agccattgaa accgaagcga aaaaacgtgg ctggtgggtg aaagttgaaa cccgtggttc
    41341 tgttggcgcg ggtaatgcaa tcactcccga ggaagtagcc gcagcggatc tggtgattgt
    41401 ggcggcagat atcgaagtgg atctggcgaa atttgctggt aaaccgatgt atcgtacctc
    41461 taccggtctg gcgctgaaga aaaccgcgca ggaactggat aaagcggttg ctgaagcaac
    41521 gccgtatgaa ccggcgggca aagctcaaac ggcgacctct gaaggtaaga aagagagtgc
    41581 aggcgcatac cgtcacttgc tgacgggcgt ctcttacatg ctgccgatgg tcgttgcagg
    41641 tggtctgtgt atcgcgcttt cttttgcttt tggtatcgaa gcgtttaaag agccgggtac
    41701 gttggcagcg gcgctgatgc agattggtgg tggttcagcc tttgcgctga tggtgccggt
    41761 actggcaggt tacattgcct tctccattgc cgatcgtccg ggtctcacgc cgggtctgat
    41821 tggcggtatg ctggcggtca gcaccggttc tggcttcatt ggcggtatta ttgcgggctt
    41881 cctggctggt tacattgcga agttaatcag tacgcaactg aaactgccac agagtatgga
    41941 ggcgctgaaa ccgatcctga tcattccgct aatttccagt ctggtggtcg gtctggcgat
    42001 gatctacctg atcggtaaac cggttgctgg cattctcgaa gggttgactc actggctgca
    42061 gaccatgggg actgcgaatg cggttctgct gggggcgatc ctcggtggca tgatgtgtac
    42121 tgacatgggc ggtccggtaa ataaagcagc gtacgcattc ggtgtgggtc tgctgagtac
    42181 tcaaacctat ggcccgatgg cggcgattat ggcggcaggt atggtgccac cgctggcaat
    42241 gggcctggca acaatggtgg cgcgtcgcaa attcgacaaa gcgcagcagg aaggtggcaa
    42301 agccgctctg gtactgggac tgtgctttat ttcggaaggt gcaattccgt ttgctgctcg
    42361 tgatccgatg cgtgtgctgc cgtgctgtat cgtgggtggg gcgctgactg gcgcaatctc
    42421 aatggcgatt ggtgcgaaac tgatggcacc gcacggtggt ctgtttgttc tgctgatccc
    42481 tggcgctatt acgccggtat tgggttacct ggtagcaatt attgccggta cgctggtggc
    42541 gggtttggcc tatgccttcc tgaaacgtcc ggaagtggac gcagtagcga aagccgcgta
    42601 ataaaaggtg tgttacaggg cagaaattta ctgccctgta tcattggatg agcgcaaaga
    42661 gaatgaccgg tttgtgaatg taattttatg aatttaaatg gttttatctt tcagtagaaa
    42721 cctgaaacgt tcatcaaaca ctaaggttat ttcctcaagg gcagattctt ttctgccctt
    42781 ttttagtgtt cctgttctgt gcatcccgtc acaaattcct cgcttttgta attctggcat
    42841 ttaatcgcat tttgtgagcc tgctcaaatc tgcccgcttt gcctctctgg cggcaattta
    42901 ctttacgtaa aacatgcata ctatgagcac atgtttaaat atgaacacat gtttaaactg
    42961 tctttgcagt atctatattc ttgtggattg acagggttgg taatttttta ccagaggacg
    43021 tgctatgcgc gaaaaggatt atgtcgtaat tataggttcg gcgaatattg atgtcgccgg
    43081 atattcacat gaatcattaa attatgcgga ttcaaatcca ggtaaaataa aatttacacc
    43141 tggtggagta gggcgaaata ttgcacaaaa cctggcgttg ctgggtaaca aagcctggtt
    43201 actgagcgca gtaggtagtg atttttatgg tcaatcgctg ctaacgcaaa ccaatcaatc
    43261 tggcgtttat gtcgataaat gcctgattgt gccgggagaa aatacgtcga gttatttatc
    43321 attactcgat aataccggtg aaatgctggt tgctataaat gacatgaata ttagcaacgc
    43381 tattacagct gaatatctcg cacagcaccg tgaatttatt cagagggcaa aggtcattgt
    43441 cgcggactgt aatatcagtg aagaggcact ggcatggatt ctggataatg ctgccaacgt
    43501 acccgtattt gtcgatccgg tttcagcgtg gaaatgtgtc aaagtgcgcg accgtctaaa
    43561 tcagatccac actctgaagc caaaccgcct tgaagcggaa acactgagtg ggattgcgct
    43621 gtcagggcgt ggagatgtgg caaaagttgc tgcctggttc catcaacatg gcctgaaccg
    43681 actggtattg agcatgggcg gcgatggcgt ttattacagt gatatcagcg gtgaaagtgg
    43741 ctggtctgcc ccgatcaaaa ccaatgttat taatgttacc ggagcgggcg atgccatgat
    43801 ggcgggactt gcttcgtgtt gggtagacgg aatgccgttt gccgaatctg ttcgtttcgc
    43861 acagggatgt tcgtcaatgg cgctctcctg tgaatacacc aataaccccg atttatcgat
    43921 tgccaacgtt atatcgttag tggagaacgc agaatgtctg aattaaaaat ttcccctgaa
    43981 ttattacaaa tttccccgga agtgcaggac gctttaaaaa acaaaaaacc ggttgttgcg
    44041 ctggaatcga ccataatttc tcacggaatg ccattcccac aaaatgccca aaccgcaatt
    44101 gaagtagaag aaactattcg taaacagggc gcagtaccag ccactatcgc cattattggc
    44161 ggtgtgatga aagtgggctt aagtaaagaa gaaatcgaat tgctgggccg tgaaggacat
    44221 aacgtgacca aagttagtcg tcgcgattta ccttttgttg ttgccgccgg aaaaaatggc
    44281 gcaaccactg tggcttcaac gatgattatt gcggcgcttg ccggaattaa agtatttgcc
    44341 accgggggaa ttggtggtgt gcatcgcggg gcggaacata ccttcgatat ttctgccgat
    44401 ttacaggaac tggcaaatac caatgtcact gttgtttgtg cgggggcaaa atctattctc
    44461 gatttaggat taacaacaga atatttagaa accttcggtg taccgttaat tggttatcag
    44521 acaaaagcgc tgcctgcgtt tttctgccga accagcccat ttgaagtcag cattcgtctt
    44581 gacagtgccc gcgaaattgc tcgcgcgatg gcagtgaagt ggcaaaccgg tttgaacggc
    44641 ggcctggtag tggcgaatcc gatcccggaa cagtttgcga tgccagaaga aaccatcaat
    44701 gcagcaattg atcaagccgt tgctgaagcc gaagaacagg gtgttattgg gaaagagagt
    44761 acaccgttcc tgctggcgcg tgttgcggag ctaacaggcg gtgatagcct gaaatccaat
    44821 attcaactgg tctttaataa cgccattctg gcgagcgaaa ttgccaaaga atatcagcgt
    44881 ctcgtgggtt aatttctggc gggcagaaag ccgtcgcccg tactcatcaa acataccaaa
    44941 acctggcgct gacgccgggt ttactggcat gaagggaaaa tcattatgga tataatgaga
    45001 agtgttgtgg ggatggtggt attgctggca attgcctttg tgttgtcggt aaataaaaag
    45061 agcatcagct tgcgcacggt tggcgctgcg ctgctgctgc aaattgccat tggtggcatc
    45121 atgctctatt ttcccccggg aaaatgggca gtagagcagg cggcattagg cgtccataaa
    45181 gtgatgtcct acagtgacgc aggaagtgcc tttatttttg gttcgctggt cggtcccaaa
    45241 atggatgttc tgtttgacgg tgcgggcttt atcttcgcct ttcgcgtact cccggcgatt
    45301 attttcgtta ccgcgcttat cagcttgctg tactacattg gcgtaatggg gctgctgatt
    45361 cgtattcttg gcagtatttt tcagaaagcc ctcaacatca gcaaaatcga atcttttgtc
    45421 gcagtcacca ctattttcct cgggcaaaat gagatcccgg cgatcgttaa accttttatt
    45481 gatcgcatga atcgcaacga gctatttacg gctatttgta gcggcatggc gtctattgcg
    45541 ggttcgatga tgattggcta tgccggaatg ggcgtgccga ttgactattt gttagccgct
    45601 tcgctgatgg ctattcctgg cggtatcctg tttgcacgta ttcttagtcc ggcgacggaa
    45661 ccttcgcaag ttacgtttga aaatctgtcg tttagcgaaa caccgccaaa aagcattatt
    45721 gaagctgcgg cgaacggtgc aatgaccggg ctgaaaatcg ccgccggtgt ggcgacggtg
    45781 gtaatggcat ttgtggcaat tattgcgctg atcaacggca ttattggtgg agttggcggc
    45841 tggtttggtt ttgccaatgt ctctctggaa agcattttcg gctatgtgct ggccccgttg
    45901 gcatggatta tgggggtgga ctggagtgat gcaaatcttg ccggaagttt gattgggcag
    45961 aaactggcga ttaatgagtt tgttgcttat ctcaatttct cgccctatct gcaaacgagc
    46021 ggcacgctgg atgtcaagac cattgccatt atctcgttcg ccctctgtgg gtttgcaaac
    46081 tttggttcta tcggtgttgt cgttggcgca ttttcggcta tttcgccaaa acgtgctcca
    46141 gaaatcgccc agcttggttt acgggcgctg gcagcagcaa cgctttccaa cctgatgagt
    46201 gcgactattg ccgggttctt tattggtctg gcgtaatgct cgatttgtag accggataaa
    46261 acgtattaag cgttgcatcc ggcaatcgcg ctattgcaaa taatttttac tgcatcatcc
    46321 cggagaattt attctccggg tccatctcca gcgccagtcc tgacaactgc tttctgtttt
    46381 taatgagata ccctttcttg cttttagtta ataaaccgtc gtgaatgaat tgcgcgagaa
    46441 catataaaag atgtcgataa gaaacgccta aatactctgc cgcttgcgta tgcttttcgt
    46501 gataaagatc accttcctgc gagagtaata taaatgctgc caggcgattc actaacggaa
    46561 atgattgatt ctgagttaaa gaaacaatat tacgataatt tttatgactt aaggtgacgc
    46621 agagttttcg taaaaatagc gtgtcgttta ataaccgcgg gcggtaatgt ttcataggga
    46681 gcgcaaggca ccaacactct tcaatagcct gcaccgcacg cggttcatgg tctttgtcga
    46741 ttaactcaat ctcgccaata aaacaggggg cggcaaagaa atcgatcagc gacacgcgac
    46801 cattagctag cgtggcgtaa agcctggcgc ggcctcgcgt caggtaaaac agccaggacg
    46861 gctgttgccc ttcctgcaca atgtagtcac gcgctaaaaa atgaaacagc cgcgtatcgg
    46921 ctgaaacatc cgttaaaaag caatccttaa acgcggattc actcatcaat tgctgcttaa
    46981 gatcattatt gtggatttct ttcacgatgt ttatccgcta tgagatttct catatttagc
    47041 atacgtacgc ggttggtaag attcaatcat cggcccggtc gcattttctt tgctgtagaa
    47101 aaaataacgc aactggaaac agaggaaata aacaatggaa aagagaaaaa ttattctgga
    47161 ttgtgatccg ggtcatgatg atgctattgc tataatgatg gcggcgaaac atccggcaat
    47221 agatttatta ggcatcacta ttgtagcggg taatcagacg cttgataaaa cattaattaa
    47281 tggcctgaat gtttgccaga aactggagat taatgttccg gtttatgcgg ggatgccgca
    47341 acccattatg cgtcaacaaa tcgttgccga taatattcac ggtgaaaccg gactggatgg
    47401 cccggtattc gagccgctga cccgccaggc agaaagtacc cacgcggtga aatacatcat
    47461 tgataccctg atggcaagcg atggcgatat cactctggtg ccggttggtc cgctttcaaa
    47521 tatcgcggtg gcaatgcgta tgcaacccgc gatcctgccc aaaatccgtg aaattgtgct
    47581 aatgggcggc gcttacggta caggcaactt cacgccatct gccgagttca acatctttgc
    47641 tgacccggaa gcagcccgcg tggtgttcac ctctggcgtt ccattagtga tgatgggcct
    47701 cgatctcacc aaccagaccg tttgcacccc ggacgtgatt gctcggatgg aaagggcagg
    47761 cggccctgcc ggagagctgt tcagcgacat catgaacttc actctcaaaa cgcagttcga
    47821 aaactacggc ctggctggcg gcccggtgca cgacgccacc tgcatcggtt atctgattaa
    47881 ccctgatggc attaaaaccc aggagatgta cgtcgaagtg gacgtcaaca gtggcccttg
    47941 ctatgggcgc accgtctgcg acgagctggg cgttcttggc aagcccgcca ataccaaagt
    48001 cggcatcact attgataccg actggttctg gggcttagtc gaagagtgcg tgcgcggcta
    48061 catcaaaacc cattaagcgt tgataacaag ccgggccgat agcgcaagat gcaggcccgg
    48121 ctatttctga tgggcagaaa gccgtcgccc aatatgacat gaagagataa tcactatgga
    48181 tgtcatgaga agtgttctgg gaatggtggt attgctggca attgcatttt tgctgtcagt
    48241 aaacaagaag aagatcagcc tgcgtaccgt tggcgcggcg ttagtgttac aggttgtgat
    48301 tggcggcatt atgctttggt taccgccagg gcgttgggtc gctgaaaaag tcgcttttgg
    48361 cgtgcataaa gtgatggcgt acagcgacgc gggtagcgca tttatcttcg gttcgctggt
    48421 cgggccgaaa atggacacgc tgtttgatgg cgcaggattt atctttggtt tcagagtatt
    48481 accggcaatt atcttcgtta cagcactggt gagtattctc tactacatcg gtgtgatggg
    48541 gattttaatt cgcattctcg gcggtatctt ccagaaagcg ttaaatatca gcaagatcga
    48601 gtcattcgtc gcggtcacta ccattttcct cgggcaaaac gaaattccgg caatcgtcaa
    48661 accctttatc gatcgtctga atcgcaatga attatttaca gcgatttgta gtggcatggc
    48721 ctcgattgct ggttcgacaa tgattggtta cgccgccctg ggcgtacctg tggaatattt
    48781 gctggcggca tcgttaatgg cgatcccagg cgggatcttg tttgcccgcc tgttaagccc
    48841 ggctacggaa tcttcgcagg tttcctttaa taacctctct ttcaccgaaa caccgccaaa
    48901 aagcattatt gaagccgctg cgacaggggc aatgaccggg ctgaaaatcg ccgccggtgt
    48961 agcgacagtt gttatggcat ttgtcgccat cattgcgtta attaatggta ttatcggcgg
    49021 cgttggcggc tggtttggtt ttgcacatgc ctcgctggag tccattttag gttacctgtt
    49081 ggccccattg gcgtgggtga tgggggttga ctggagtgat gcaaatcttg ccgggagttt
    49141 gattgggcag aagctggcga tcaatgaatt tgtcgcttat ctcaatttct cgccatatct
    49201 gcaaacgggt ggcactctgg atgctaaaac cgtggcgatt atttctttcg cgttgtgcgg
    49261 tttcgctaac tttggttcta tcggggtggt ggtgggggcg ttttctgcgg ttgcgccaca
    49321 ccgtgcgccg gaaatcgccc aacttggttt acgcgcgctg gcggcggcga cactttctaa
    49381 cctgatgagt gctactattg caggattctt tattggttta gcgtagtgcg caatttatga
    49441 gccggataag atgcggtcaa gcgtcgaatc cggcaacatt gttacaccat tggcactaat
    49501 gaaagcgcgt tatcggcaga cagggtaggg ttgtttaagc tgccgctggc gcgcgaaatt
    49561 gccgcgcagg ctaccgcaaa acgggcgctg tcgcggaaac tgtatccttc aagaaagcta
    49621 tataccaagc ccgccataaa accatcgtca gcaccaaaac tgtctactgt cgtatgcgct
    49681 ggcgcggtca gcaaaaattg ttctccatcc ttatcgctgc aaaatacaga ttcatctttc
    49741 agataaacaa aaatgcgttt aacaccttgt tgatgcagcg aatttacagc tctgatgcga
    49801 tcgttgtcgt ctttaatcgg ttgcccccac aaaatttcca gttcattttg tgttggtttc
    49861 agagtgtgga tccggctgta ccagtttttt actttgttcg ctttaaattc ggaaacggta
    49921 tcgacaaaca tcggaatttc atcagcaatg gtaaagaccc actccagggc ttcaggtgtg
    49981 agattacaat cagccagcac cacaccagca tggcgaatca agtcccgtga agtattgagc
    50041 agttgaggcg ttagctgttg cagaatatgt gtgtcattga ttgccaggat agtttcttcc
    50101 tgcttattag cgatagcgag ataagttgca gtgctgtgtc catgcagacg aatgcagttt
    50161 gaaacgttca cgcccgcgcg gcgcgtttct tccagtaatg tctcgccgta aaaatcgttg
    50221 ccaatagctg atattaaatg cacatcccga ccaagcagag ccaggttatg cgcgatatta
    50281 cgtccgacgc cgcctgcgga acagtgaaca gaacctggat gagaggctgc ttgcgggtaa
    50341 cggatatccg ccattccgcg aatatccata ttgattgcac ccacaaccac acagtaatcc
    50401 tgctcggtaa ggatatatcc tttgccttta atcaaaccct tgcgcatcaa atccataata
    50461 tgagcggcaa cacgtgagcg gctgatttgc agaatatcgg cgatttcatt ctgttgaatc
    50521 agcgggttac gccgtaaaat tttaagaatc tgcttttccc tgtcgttcat cgatcaggcc
    50581 accgcttttt cagtttgttg cgctttcagc caggcgatct cttctgccca gatatccggg
    50641 ttgatggttt caaggatcag cggaatgccg tcgaaacggt cgtcctgcat gatccagcgg
    50701 aacgcatcat gaccgatatt gccttcaccg aggctatgat ggcggtcaac gcggctgcca
    50761 aaggtgcttt tcgcatcgtt aaggtgcatc ccgcgcagat acttaaagcc aacaatacgg
    50821 gcaaaatccg cgaacgtttt ctcgcattcg gctggagtac gcaaatcata cccggcagcg
    50881 aaagcatggc aggtatcaat gcagacgccg acacgggatt tatcttccac gccgtcgata
    50941 atcgccgcga gatgttcgaa tttaaaccct aagttactgc cctgaccggc ggtgttttca
    51001 atcaccgctg ttacaccttg agttttatcc agcgcaatgt tgatggattc ggcaatacgc
    51061 gcaaggcaat cctcttctga aatctgcatc agatggctgc cagggtggaa gttgagcaaa
    51121 gaaagcccca gttgttcgca acgctgcatt tcatctataa aggcatcgcg cgatttttcc
    51181 agagcttcag tgaccggatg tccgaggtta atcagatagc tgtcgtgggg aagaatttgc
    51241 gccgatgtgt agtgatattt ttcacaggcc gctttgaatt catcgatggt ttgcgtcgtg
    51301 agcggtgcgg cacgccactg acgttggttt ttggtgaaca aggcaaacgc ggttgcgtcg
    51361 atttcggcgg cgcgaattgc ggcatttgcc agaccgccag cagcactaac gtgcgctcca
    51421 atgtatttca tgcgaggact cctgttaaac ccgctggagg aaaacggtaa tgatagcggg
    51481 ttaacaggag caagatgtag tggtttatgc gatgacgctt tgaatcacat agttaatcgc
    51541 accaccacca acaatcagcc aggcaaacag taccagtgcc atcagcagag gtttcgcccc
    51601 ggcttttttc agcgcgctga cgtgagtggt cagacccagc gccgccatcg ccattgccag
    51661 caggaaggta tccagcgtta ccagcatgtt caccacgctc tgcggtaaca ggtggaacga
    51721 gttaaagatg gcaacgacga tgaacaatat ggcaaaccac ggaatagtga ttttgctttt
    51781 ctcgccgctg ttcgccccag acagctgttt aacacgcgcc gccagcagga tgaggaacgg
    51841 agccagcatc atcacgcgca gcattttgga aataactgct gcgttttccg catccgggct
    51901 gatggcatga cctgccgcca ccacctgcgc cacttcgtgc acagtagaac caatgtagat
    51961 accgaaagtt tccggactaa accattgaga catcagcgga tatatcgccg ggtagaggaa
    52021 aatcgcgacg gtcccgaaga taacaacggt tgcaacagcc acggttactt tactggcttc
    52081 cgctttcact accggctcag tcgccagtac cgcggcagca ccacagatac tgctaccggc
    52141 accgatcaac cagctggtgt gcttatccag accaaacact ttctgcccca ggaagcaagc
    52201 cagcaggaag gtactggaca gcgtcagcac gtcaatgatg atcccactga taccgacatc
    52261 ggcaatttgc gagaacgtca gacggaagcc ataaagaatg atacccagac gtaataaata
    52321 ttgcttggca aacagcacac caccgtcaca gcttttccag atgtgcggat agatggtgtt
    52381 gcctaaaacc atccccaaca agattgcgag ggtgagggca ctaaacccgg cacccgcaac
    52441 cgcgggaatg gaaccacccc acagggcgac cccggtgata actgcactca gggctaaccc
    52501 cggaataaaa tgccacagtg tacgatgttg tttctgtaag gtgatattcg tcataaccct
    52561 ctccttt
//
LOCUS       DAZFXT010000017        51125 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.017, whole genome shotgun sequence.
ACCESSION   DAZFXT010000017 DAZFXT010000000
VERSION     DAZFXT010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51125)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51125)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51125
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.017"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(375..851)
                     /gene="ybhB"
                     /locus_tag="ACHKW0_001141"
     CDS             complement(375..851)
                     /gene="ybhB"
                     /locus_tag="ACHKW0_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HFO7474009.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDEVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(910..2199)
                     /gene="bioA"
                     /locus_tag="ACHKW0_001142"
     CDS             complement(910..2199)
                     /gene="bioA"
                     /locus_tag="ACHKW0_001142"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HFO7474010.1"
                     /translation="MTTDDIAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQNRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2286..3326
                     /gene="bioB"
                     /locus_tag="ACHKW0_001143"
     CDS             2286..3326
                     /gene="bioB"
                     /locus_tag="ACHKW0_001143"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HFO7474011.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3323..4477
                     /gene="bioF"
                     /locus_tag="ACHKW0_001144"
     CDS             3323..4477
                     /gene="bioF"
                     /locus_tag="ACHKW0_001144"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HFO7474012.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIAADRLSHASLLEAASLSPSQLRRFAHNDVTHLAR
                     LLASPCPGQQMVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            4464..5219
                     /gene="bioC"
                     /locus_tag="ACHKW0_001145"
     CDS             4464..5219
                     /gene="bioC"
                     /locus_tag="ACHKW0_001145"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010340 - carboxyl-O-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HFO7474013.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPGAIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQKQGRYPLTYHLFLGVIARE"
     gene            5212..5889
                     /gene="bioD"
                     /locus_tag="ACHKW0_001146"
     CDS             5212..5889
                     /gene="bioD"
                     /locus_tag="ACHKW0_001146"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7474014.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6468..8489
                     /gene="uvrB"
                     /locus_tag="ACHKW0_001147"
     CDS             6468..8489
                     /gene="uvrB"
                     /locus_tag="ACHKW0_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HFO7474015.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8681..9589)
                     /gene="yvcK"
                     /locus_tag="ACHKW0_001148"
     CDS             complement(8681..9589)
                     /gene="yvcK"
                     /locus_tag="ACHKW0_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HFO7474016.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      9854..9998
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            9986..10975
                     /gene="moaA"
                     /locus_tag="ACHKW0_001149"
     CDS             9986..10975
                     /gene="moaA"
                     /locus_tag="ACHKW0_001149"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HFO7474017.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            10997..11509
                     /gene="moaB"
                     /locus_tag="ACHKW0_001150"
     CDS             10997..11509
                     /gene="moaB"
                     /locus_tag="ACHKW0_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HFO7474018.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11512..11997
                     /gene="moaC"
                     /locus_tag="ACHKW0_001151"
     CDS             11512..11997
                     /gene="moaC"
                     /locus_tag="ACHKW0_001151"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HFO7474019.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            11990..12235
                     /gene="moaD"
                     /locus_tag="ACHKW0_001152"
     CDS             11990..12235
                     /gene="moaD"
                     /locus_tag="ACHKW0_001152"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HFO7474020.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12237..12689
                     /gene="moaE"
                     /locus_tag="ACHKW0_001153"
     CDS             12237..12689
                     /gene="moaE"
                     /locus_tag="ACHKW0_001153"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HFO7474021.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            12826..13530
                     /gene="ybhL"
                     /locus_tag="ACHKW0_001154"
     CDS             12826..13530
                     /gene="ybhL"
                     /locus_tag="ACHKW0_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO7474022.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            13675..14379
                     /locus_tag="ACHKW0_001155"
     CDS             13675..14379
                     /locus_tag="ACHKW0_001155"
                     /inference="COORDINATES: protein motif:HMM:NF013214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HFO7474023.1"
                     /translation="MNRQSTATQLQQKSASLSKMLLLIWWLTAAGVWATAGCAWIFQH
                     LHWVTSQKSPGAFVFALAALIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLY
                     TFSSIATVMGITGGMFAAMALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSF
                     SVWLASILTVFIWGITAACEATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVL
                     FRITVTVLEFINGLVW"
     gene            14449..15162
                     /locus_tag="ACHKW0_001156"
     CDS             14449..15162
                     /locus_tag="ACHKW0_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HFO7474024.1"
                     /translation="MNSRSANNITNDFQHEARILNSIWLITALGLVTTAGLAWVAKYL
                     EITATKYDSPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAP
                     VPAIIVVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERP
                     VWVISCLMIVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(15198..16154)
                     /gene="ybhN"
                     /locus_tag="ACHKW0_001157"
     CDS             complement(15198..16154)
                     /gene="ybhN"
                     /locus_tag="ACHKW0_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HFO7474025.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(16154..17395)
                     /gene="clsB"
                     /locus_tag="ACHKW0_001158"
     CDS             complement(16154..17395)
                     /gene="clsB"
                     /locus_tag="ACHKW0_001158"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HFO7474026.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHYRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(17392..18153)
                     /gene="ybhP"
                     /locus_tag="ACHKW0_001159"
     CDS             complement(17392..18153)
                     /gene="ybhP"
                     /locus_tag="ACHKW0_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7474027.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            18286..18696
                     /gene="ybhQ"
                     /locus_tag="ACHKW0_001160"
     CDS             18286..18696
                     /gene="ybhQ"
                     /locus_tag="ACHKW0_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HFO7474028.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18658..19764)
                     /gene="ybhR"
                     /locus_tag="ACHKW0_001161"
     CDS             complement(18658..19764)
                     /gene="ybhR"
                     /locus_tag="ACHKW0_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7474029.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19775..20908)
                     /gene="ybhS"
                     /locus_tag="ACHKW0_001162"
     CDS             complement(19775..20908)
                     /gene="ybhS"
                     /locus_tag="ACHKW0_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7474030.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20901..22637)
                     /gene="ybhF"
                     /locus_tag="ACHKW0_001163"
     CDS             complement(20901..22637)
                     /gene="ybhF"
                     /locus_tag="ACHKW0_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HFO7474031.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHAEGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22630..23625)
                     /gene="hlyD"
                     /locus_tag="ACHKW0_001164"
     CDS             complement(22630..23625)
                     /gene="hlyD"
                     /locus_tag="ACHKW0_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HFO7474032.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(23628..24299)
                     /gene="cecR"
                     /locus_tag="ACHKW0_001165"
     CDS             complement(23628..24299)
                     /gene="cecR"
                     /locus_tag="ACHKW0_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HFO7474033.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24528..25892
                     /gene="rhlE"
                     /locus_tag="ACHKW0_001166"
     CDS             24528..25892
                     /gene="rhlE"
                     /locus_tag="ACHKW0_001166"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HFO7474034.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     TAAQ"
     gene            complement(26124..26606)
                     /gene="ybiA"
                     /locus_tag="ACHKW0_001167"
     CDS             complement(26124..26606)
                     /gene="ybiA"
                     /locus_tag="ACHKW0_001167"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HFO7474035.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHPELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26726..28876
                     /gene="dinG"
                     /locus_tag="ACHKW0_001168"
     CDS             26726..28876
                     /gene="dinG"
                     /locus_tag="ACHKW0_001168"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HFO7474036.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28904..29866
                     /gene="ybiB"
                     /locus_tag="ACHKW0_001169"
     CDS             28904..29866
                     /gene="ybiB"
                     /locus_tag="ACHKW0_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HFO7474037.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            30007..31092
                     /gene="hcxB"
                     /locus_tag="ACHKW0_001170"
     CDS             30007..31092
                     /gene="hcxB"
                     /locus_tag="ACHKW0_001170"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HFO7474038.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCSAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            31159..31218
                     /gene="ybiE"
                     /locus_tag="ACHKW0_001171"
     CDS             31159..31218
                     /gene="ybiE"
                     /locus_tag="ACHKW0_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbiE"
                     /protein_id="HFO7474039.1"
                     /translation="MRLSANRQGNTCAGRSVNA"
     gene            complement(31321..31581)
                     /gene="ybiJ"
                     /locus_tag="ACHKW0_001172"
     CDS             complement(31321..31581)
                     /gene="ybiJ"
                     /locus_tag="ACHKW0_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YbiJ"
                     /protein_id="HFO7474040.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31846..32112)
                     /gene="ybiI"
                     /locus_tag="ACHKW0_001173"
     CDS             complement(31846..32112)
                     /gene="ybiI"
                     /locus_tag="ACHKW0_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HFO7474041.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(32186..32863)
                     /gene="ybiX"
                     /locus_tag="ACHKW0_001174"
     CDS             complement(32186..32863)
                     /gene="ybiX"
                     /locus_tag="ACHKW0_001174"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HFO7474042.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32905..35187)
                     /gene="fiu"
                     /locus_tag="ACHKW0_001175"
     CDS             complement(32905..35187)
                     /gene="fiu"
                     /locus_tag="ACHKW0_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HFO7474043.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(35451..35711)
                     /gene="mcbA"
                     /locus_tag="ACHKW0_001176"
     CDS             complement(35451..35711)
                     /gene="mcbA"
                     /locus_tag="ACHKW0_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein McbA"
                     /protein_id="HFO7474044.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            35987..36913
                     /gene="rlmF"
                     /locus_tag="ACHKW0_001177"
     CDS             35987..36913
                     /gene="rlmF"
                     /locus_tag="ACHKW0_001177"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008988 - rRNA
                     (adenine-N6-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HFO7474045.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(36910..39135)
                     /gene="ybiO"
                     /locus_tag="ACHKW0_001178"
     CDS             complement(36910..39135)
                     /gene="ybiO"
                     /locus_tag="ACHKW0_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HFO7474046.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(39252..39974)
                     /gene="glnQ"
                     /locus_tag="ACHKW0_001179"
     CDS             complement(39252..39974)
                     /gene="glnQ"
                     /locus_tag="ACHKW0_001179"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HFO7474047.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(39971..40630)
                     /gene="glnP"
                     /locus_tag="ACHKW0_001180"
     CDS             complement(39971..40630)
                     /gene="glnP"
                     /locus_tag="ACHKW0_001180"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HFO7474048.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(40769..41515)
                     /gene="glnH"
                     /locus_tag="ACHKW0_001181"
     CDS             complement(40769..41515)
                     /gene="glnH"
                     /locus_tag="ACHKW0_001181"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015599 - ATPase-coupled L-glutamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1903803 - L-glutamine import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HFO7474049.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(41919..42422)
                     /gene="dps"
                     /locus_tag="ACHKW0_001182"
                     /gene_synonym="pexB"
     CDS             complement(41919..42422)
                     /gene="dps"
                     /locus_tag="ACHKW0_001182"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HFO7474050.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            complement(42721..43608)
                     /gene="rhtA"
                     /locus_tag="ACHKW0_001183"
     CDS             complement(42721..43608)
                     /gene="rhtA"
                     /locus_tag="ACHKW0_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HFO7474051.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            43843..43908
                     /locus_tag="ACHKW0_001184"
     CDS             43843..43908
                     /locus_tag="ACHKW0_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474052.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            43961..44476
                     /gene="ompX"
                     /locus_tag="ACHKW0_001185"
     CDS             43961..44476
                     /gene="ompX"
                     /locus_tag="ACHKW0_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HFO7474053.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(44525..46108)
                     /gene="opgE"
                     /locus_tag="ACHKW0_001186"
     CDS             complement(44525..46108)
                     /gene="opgE"
                     /locus_tag="ACHKW0_001186"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7474054.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(46380..46508)
                     /gene="mntS"
                     /locus_tag="ACHKW0_001187"
     CDS             complement(46380..46508)
                     /gene="mntS"
                     /locus_tag="ACHKW0_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HFO7474055.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            46694..47161
                     /gene="mntR"
                     /locus_tag="ACHKW0_001188"
     CDS             46694..47161
                     /gene="mntR"
                     /locus_tag="ACHKW0_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HFO7474056.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            47158..48276
                     /gene="ybiR"
                     /locus_tag="ACHKW0_001189"
     CDS             47158..48276
                     /gene="ybiR"
                     /locus_tag="ACHKW0_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HFO7474057.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLCAALVGYVLLVILPAN"
     gene            complement(48335..49255)
                     /gene="ldtB"
                     /locus_tag="ACHKW0_001190"
     CDS             complement(48335..49255)
                     /gene="ldtB"
                     /locus_tag="ACHKW0_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7474058.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            49474..51066
                     /gene="ybiT"
                     /locus_tag="ACHKW0_001191"
     CDS             49474..51066
                     /gene="ybiT"
                     /locus_tag="ACHKW0_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HFO7474059.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
BASE COUNT        12271 a        12985 c        13635 g        12234 t
ORIGIN      
        1 ttttaatgaa aatgagcata aaaccaagac atataatgct tggcagcttt atgctcttct
       61 gattggctgt atgtttgatg acaaaaaatt agcgcaagag gacaaaaaat caccttgcgc
      121 taatgctctg tctcaggtca ctaatactat ctaaatagtt gattcatagt gactgggtat
      181 gttgtgtttt gtcgtatttt gtagtctatt atttagactg agtcaattat aagacgttga
      241 tattaatggt ttttactgtt tcacattcaa cttttttata ctaacttgag cgaaacggga
      301 aggtaaaaag acaaaaagtt gtttttaata cctttaagtg ataccagatg gcattgcgcc
      361 atctggcaga gtgattaact aaacatcgca gtaatcgagg cgcttgccag agagtggaaa
      421 tgaacgttaa acccgaccat cgcgccgctg gcaccttcat cgacatcaat acgttctaca
      481 tccagcgcgt gaacggtaaa aatgtagcga tgagtttcgc ctttcggcgg tgctgcgcca
      541 tcgtatccgg ttttaccaaa atcggtacgc gtctgcaaaa cgccgtctgg cattgctacc
      601 agaccagagc caaacccttg cggtaatacg cgtgtatcag cgggtaagtt aacaactacc
      661 cagtgccacc agccggagcc ggttggcgca tccgggtcgt agcaggtgac aacaaaactt
      721 ttcgttcccg caggaacttc atcccacgcc agatgcggtg aaatattatc gccatcgtaa
      781 cccatgccgt taaagacatg acgatgcggc aacttatcgc catcgcgcag atcgttactg
      841 atgagtttca tgaaccctcc tttcttgttt gcagaaagtg tagccagaaa ccctcacgct
      901 gacttcccgt tattggcaaa aaaatgtttc atcctgtacc gcgcggttaa ccgctgcggt
      961 cagacgctgc aactgttgcg ggagaataat atagggcggc atcaggtaaa tcagtttgcc
     1021 aaaaggccgg atccagacac cctgttcgac aaagaatttt tgcagcgccg ccatattcac
     1081 cggacgagtg gtttcgacca caccgattgc ccccagtacg cgcacatcgg caaccatttc
     1141 ggcatcacgg gcgggggcaa gttgctcgcg cagctgtact tcaatagccg ccacctgctg
     1201 ctgccattcg ccggattcga taatcgccag gctggcgttt gctgccgcgc aggccagcgg
     1261 attgcccata aaagttggcc cgtgcataaa gcaaccggct tcgccgttac tgatggtttc
     1321 cgcaacctcg cgcgtggtga gcgtggcgga aagggtcatt gtgccgccgg ttaaggcttt
     1381 accgaggcac aaaatgtccg gcgcgatttc tgcatattca caggcaaaca gtttgccggt
     1441 acgaccaaat ccggtggcga tctcgtcggc aatcagcaag ataccttcgc gatcgcacat
     1501 tttgcggatt cgttttaacc attccggatg gtacatgcgc atcccgcctg cgccctggac
     1561 aatcggctca atgatcaccg ctgcgatttc atgacgatgc gccgccatca ggcgggcaaa
     1621 accaaccata tcacgctcat cccattcacc atccatgcgg ttttgcgggg cgggagcaaa
     1681 caagttttct ggcagatagc ctttccacag gctgtgcatt gagttgtccg gatcgcacac
     1741 cgacatcgcg ccaaaggtat cgccatgata accattgcgg aaggtcagaa aacgctggcg
     1801 cgcttcgcct ttggcttgcc agtactgcaa cgccattttc atcgccactt ccaccgctac
     1861 ggaaccggag tccgcgagaa aaacgcactc cagtggttgc ggcgtcatcg ccaccagttt
     1921 gcggcacagc tcaatggctg gcgcatgagt gataccgcca aacatcacat gcgacatggc
     1981 atcaatttgc gtcttcatcg ccgcattaag ctgcgggtga ttgtagccgt ggatcgccgc
     2041 ccaccaggac gacataccgt caaccaggcg tctgccgtca gacaaaatta gctcgcaacc
     2101 ttcggcgctc gccaccggat aaaccggcag aggggaggtc atggatgtgt atgggtgcca
     2161 gatatggcgt tggtcaaagg caatatcgtc cgttgtcata atcgacttgt aaaccaaatt
     2221 gaaaagattt aggtttacga gtctacaccg aattaacaac aaaaaacacg ttttggagaa
     2281 gccccatggc tcaccgccca cgctggacat tgtcgcaagt cacagaatta tttgaaaaac
     2341 cgttgctgga tctgctgttt gaagcgcagc aggtgcatcg acagcatttc gatcctcgtc
     2401 aggtgcaggt cagcactttg ctgtcgatta agaccggagc ttgtccggaa gattgcaaat
     2461 actgcccgca aagctcgcgc tacaaaaccg ggctggaagc cgagcggttg atggaagttg
     2521 aacaggtgct ggagtcggcg cgcaaagcga aagctgcggg atcgacgcgt ttctgtatgg
     2581 gcgcggcgtg gaagaatccc cacgaacgcg atatgccgta cctggaacaa atggtgcagg
     2641 gggtaaaagc gatggggctg gaggcgtgta tgacgctggg cacgttgagt gaatctcagg
     2701 cgcagcgcct tgcgaatgcc gggctggatt actacaacca caacctggac acctcgccgg
     2761 agttttacgg caatatcatc accacccgca cttatcagga acgcctcgat acgctggaaa
     2821 aagtgcgcga tgccgggatc aaagtctgtt ctggcggcat tgtgggctta ggcgaaacgg
     2881 taaaagatcg cgccggatta ttgctgcaac tggcaaacct gccgacgccg ccggaaagcg
     2941 taccaatcaa catgctggtg aaggtgaaag gcacgccgct tgccgataac gatgatgtcg
     3001 atgcctttga ttttattcgc accattgcgg tcgcgcggat catgatgcca acctcttacg
     3061 tgcgcctttc tgccggacgc gagcagatga acgaacagac tcaggcgatg tgctttatgg
     3121 caggcgcaaa ctcgattttc tacggttgca aactgctgac cacgccgaat ccggaagaag
     3181 ataaagacct gcaactgttc cgcaaactgg ggctaaatcc gcagcaaact gccgtgctgg
     3241 caggggataa cgaacaacag caacgtcttg aacaggcgct gatgaccccg gacaccgacg
     3301 aatattacaa cgcggcagca ttatgagctg gcaggagaaa atcaacgcgg cgctcgatgc
     3361 gcggcgtgct gccgatgccc tgcgtcgccg ttatccagtg gcgcaaggag ccggacgctg
     3421 gctggtggcg gatgatcgcc agtatctgaa cttttccagt aacgattatt taggtttaag
     3481 ccatcatccg caaattatcc gtgcctggca gcagggtgcg gagcaatttg gcgtcggtag
     3541 cggcggttcc ggtcacgtca gcggttatag cgttgcgcat caggcgctgg aagaagaact
     3601 ggccgagtgg ctgggctatt cgcgggcact gctatttatc tctggttttg ccgctaatca
     3661 ggcagttatt gccgcgatga tggcgaaaga ggaccgtatt gctgccgacc ggcttagcca
     3721 tgcctcattg ctggaagctg ccagtttaag cccgtcgcag cttcgccgtt ttgctcataa
     3781 cgatgtcact catttggcgc gattgcttgc ttccccctgt ccggggcagc aaatggtggt
     3841 gacagaaggc gtgttcagca tggacggcga tagtgcgcca ctggcggaaa tccagcaggt
     3901 aacgcaacag cacaatggct ggttgatggt cgatgatgcc cacggcacgg gcgttatcgg
     3961 ggagcagggg cgcggcagct gctggctgca aaaggtaaaa ccagaattgc tggtagtgac
     4021 ttttggcaaa ggatttggcg tcagcggggc agcggtgctt tgctccagta cggtggcgga
     4081 ttatctgctg caattcgccc gccaccttat ctacagcacc agtatgccgc ccgctcaggc
     4141 gcaggcatta cgtgcgtcgc tggcggtcat tcgcagtgaa gagggggacg ctcggcgcga
     4201 aaaactggcg gcactcatta cgcgttttcg tgccggagtg cagggttcgc cgtttacgct
     4261 tgcggattca cgtagcgcca tccagccatt gatcgtcggt gataacagcc gtgcgttaca
     4321 gctggcagaa aaactgcgcc agcaaggttg ctgggtgacg gcgattcgcc cgccaaccgt
     4381 acccgctggc accgcgcggc tacgcctgac cttaaccgcc gcccatgaaa tgcaggatat
     4441 tgaccgtttg ctggagatgc tgcatggcaa cggttaataa acaagccatt gcagcggcat
     4501 ttggtcgggc agccgcgcac tatgagcaac acgcagatct acagcgccag agtgctgacg
     4561 tcttactggc aatgctccca cagcgtaaat acacccacgt actggacgcg gggtgtgggc
     4621 caggctggat gagccgccac tggcgagagc gtcacgcgca ggtgacggcg ttagatctct
     4681 cgccgccaat gcttgttcag gcacgccaga aggatgccgc agaccattat ctggcgggag
     4741 atatcgaatc cctgccgtta gcgactgcga cgttcgatct tgcatggagc aatctcgcgg
     4801 tgcagtggtg cggtaattta tccacggcac tccgcgagct gtatcgggtg gtgcgccccg
     4861 gaggcgtggt cgcgtttacc acgctggtgc agggatcgtt acccgaactg catcaggcgt
     4921 ggcaggcagt ggacgagcgt ccgcatgcta atcgcttttt accaccaggt gcaatcgaac
     4981 agtcactgaa cggcgtgcat tatcaacatc atattcagcc catcacgctg tggtttgatg
     5041 atgcgctcag tgccatgcgt tcgctgaaag gcattggagc cacgcatctt catgaagggc
     5101 gcgacccgcg aatattaacg cgttcgcagt tgcagcgatt gcaactggcc tggccacaaa
     5161 aacaggggcg atatcccctg acgtatcatc tttttttggg agtgattgct cgtgagtaaa
     5221 cgttattttg tcaccggaac ggataccgaa gtggggaaaa ctgtcgccag ttgtgcactt
     5281 ttacaagccg caaaggcagt aggttatcgg acggcaggtt ataaaccggt cgcctctggc
     5341 agcgaaaaga ccccggaagg cttacgtaat agtgacgcgc tggcgttaca gcacaacagc
     5401 agcctgcagc tggattacgc aacggtaaat ccttacacct tcgcagaacc cacctcgccg
     5461 cacatcatca gcgcgcaaga gggcaggccg atagaatcat tggtaatgag cgccggatta
     5521 cgcgcgcttg aacaacaggc tgactgggtg ttagtggaag gtgctggcgg ctggtttacg
     5581 ccgctttctg acactttcac ttttgcagat tgggtaacac aggaacaact gccggtgata
     5641 ctggtagttg gtgtgaaact cggctgtatt aatcacgcga tgttgactgc gcaggcaata
     5701 caacacgccg gactgactct ggcgggttgg gtggcgaacg atgttacgcc tccgggaaaa
     5761 cgtcacgctg aatatatgac cacgctcacc cgcatgattc ccgcgccgct gctgggagag
     5821 atcccctggc ttgcagaaaa tccagaaaat gcggcaaccg gaaagtacat aaaccttgcc
     5881 ttgttgtagc catcctgtat ttggttaaat tgcgagcgag atcgcgtctt cgattgactg
     5941 caatttaacc aattaaattc taaaataatc acgaaaaaaa ttttacttcc gcctcatgcg
     6001 gcgaatgtgg gaattgccca ggcggcgggg gataggggct ggagacagtt atccactatt
     6061 cctgtggata accatgtgta ttagagttag aaaacacgag gcaagcgaga gaatacgcgg
     6121 cttgcacgcg aattggcgtt aaagacggct caaagaaata tcttttattt tttaactggt
     6181 tagataaatg caatggcagt cactgaacag gcatctcttg ccataaaact gtcatcactc
     6241 atcttgacaa atgttaaaaa agccgttgct ttggggataa cccggtaagg ccggagtttt
     6301 atctcgccac agagtaaatt ttgctcatga ttgacagcgg agtttacgct gtcacagaaa
     6361 tattatggtg atgaactgtt tttttatcca gtataatttg ttggcataat taagtacgac
     6421 gagtaaaatt acatacctgc ccgcccaact ccttcaggta gcgactcatg agtaaaccgt
     6481 tcaaactgaa ttccgctttt aaaccttctg gcgatcagcc agaggcgatt cgacgtctcg
     6541 aagaggggct ggaagatggc ctggcgcacc agacgttact tggcgtgact ggctccggga
     6601 aaaccttcac cattgccaat gtcattgctg accttcagcg cccaaccatg gtacttgcgc
     6661 ccaacaaaac gctggcggcc cagctgtatg gcgaaatgaa agagttcttc ccggaaaacg
     6721 cggtggagta tttcgtctcc tactacgact actatcagcc ggaagcctat gtaccgagtt
     6781 ccgacacctt cattgagaaa gatgcctcgg ttaacgaaca tattgagcag atgcgtttat
     6841 ccgccaccaa agcgatgctg gagcgacgtg atgtggttgt agtggcgtct gtttccgcga
     6901 tttatggtct gggcgatcct gatttatatc tcaagatgat gctccatctc acggtcggta
     6961 tgattatcga tcagcgcgcg attctgcgcc gactggcgga gctgcaatac gctcgtaatg
     7021 atcaagcatt ccagcgtggt actttccgcg ttcgtggcga ggtgattgat atcttcccgg
     7081 cagaatcgga tgacattgca cttcgcgtgg aactgtttga cgaggaagtg gaacgattgt
     7141 cgttatttga cccgctaacc gggcagattg tttccaccat tccacgtttt accatctacc
     7201 cgaaaacgca ctacgtcaca ccgcgcgagc gcatcgtaca ggcgatggag gagatcaaag
     7261 aagagctggc cgccagacgc aaagtgctat tggaaaacaa caaactgctg gaagagcagc
     7321 ggctgaccca gcgtacccag tttgatctgg agatgatgaa cgagctgggc tactgttcgg
     7381 ggattgaaaa ctactcgcgc ttcctctccg gtcgtggacc gggtgagcca ccgccgacgc
     7441 tgtttgatta cctccctgcc gatgggctgc tggtggtcga tgaatctcat gtcaccattc
     7501 ctcaaattgg cggcatgtat cgcggtgacc gggcgcgtaa agagacactg gtggagtacg
     7561 gcttccgcct gccatcagcg ctggataacc gtccgcttaa gtttgaagag ttcgaagcat
     7621 tagcgccgca aaccatctat gtttcggcga cgccgggtaa ttacgagctg gaaaaatccg
     7681 gcggcgatgt ggtggatcag gtggtgcgtc caaccggatt gcttgacccg attatcgaag
     7741 tgcggccggt ggcgacacag gttgatgatc ttctttcgga gattcgtcag cgagcggcaa
     7801 ttaacgaacg cgtactggtc accacactga ccaagcggat ggcggaagat cttaccgaat
     7861 atctcgaaga acatggcgag cgcgtgcgtt atcttcactc agatatcgac accgtcgaac
     7921 gtatggagat tatccgcgac ttgcgtctgg gtgagttcga cgtgctggta gggatcaact
     7981 tactgcgcga aggtctggat atgccggaag tgtcgctggt ggcgatcctc gacgctgaca
     8041 aagaaggctt cctgcgttcc gaacgttcgt tgatccagac cattggtcgt gcggcacgta
     8101 acgttaacgg taaagcgatt ctctacggcg ataagatcac cccatcaatg gcgaaagcga
     8161 ttggcgaaac cgaacgtcgc cgtgagaaac agcagaagta caacgaggaa cacggcatta
     8221 cgccgcaagg cttgaacaag aaagtggtcg atatcctggc gctggggcag aacattgcca
     8281 aaaccaaagc gaagggcaga ggaaaatcgc gcccgattgt tgagccggat aatgtgccga
     8341 tggatatgtc gccgaaagcg ttgcagcaga aaatccatga actggaaggg ctgatgatgc
     8401 aacacgcgca gaatctggag ttcgaagaag cggcacaaat tcgtgaccag ttgcatcagt
     8461 tgcgtgagtt gtttattgcc gcatcgtaac aggatagcga agaagactga tgacaaacgg
     8521 aaaacagcct gatgtgctac gcttatcagg cctacatttt ctccgcaata tattgaattt
     8581 gcgcggtttg taggccggat aaggcgttca cgccgcatcc ggcataaaca acgcgcactt
     8641 tgtcttcaat aggaagccgg aattttcctt cggatttccg ttaacctaaa gcctgtaacg
     8701 ccttttccag cgcgttatgt aacaactggc ggtcatgacg atacggaata tcgctggcct
     8761 ccagtacctc ctggatcaca atccgctctt tcaccgccga gacatccact tttggcccga
     8821 cgatgaccgc atcaatgacc tttttaccaa catactgctc cataattgcc agcttgcttt
     8881 ccagcttcaa attagccgca ggtaaactca actcacgccc cagattgccg atataaacca
     8941 tcggcgctgg cgtgcggcgt aatgcctggg cgatttcctt cagcagcaga attggcatca
     9001 ggctggtata aaaactgcca ggcccaataa tgatgagatc cgcttcattg atagcgtgaa
     9061 ccgcctcacg cgttgcgggt acattaggcg ttaacaataa ctcttgaatc ggcgtagtta
     9121 actggtcgat attgacctcg ccgtaaactt catgcccctg atcgtcaatc gccatcagat
     9181 caacaggatg ctctgacatt ggaatcaaat gcgtatccac tttcagcaga ttacgaatta
     9241 aattgatggc ttccagaggc cgcacgctaa ggtgatccag cgcctttaac atcaagtttc
     9301 cgagattatg accggaaagt tcgccattgc caccaaaacg gtattcaaac atcgcggagg
     9361 cgacgctcgg ttccgttatc agctggttga ggcagttgcg catatcgccc caggcaatgc
     9421 cgccttctga acggcgaata cgccccgtcg agccaccatt atcggtggtg gtgacgatac
     9481 ccgttaaacg agaacccaaa gacgaaagtg atgagagaac gcgtcccagt ccatgccctc
     9541 cgccgagagc aacgacacga tcaagatcag ccagcgtacg attgcgcata tagtttcctg
     9601 gagtcagatt atccgcgcta cagtagcgca aattgcgggg aaacagcaat taacctgcca
     9661 atattgagga tacaaactat tttcttctac ctctaaagga cgatgcacgc tatgcctccc
     9721 tgatgatgta tatcaaagta aagccgccat tttcccttat tctgtagcga aatagcacga
     9781 tcatgacgct atatacatga ttacatagcg aaagtgtgga tggtaaaaat ctcatttaca
     9841 cgctagtatc ggcataacca ctaaacactc tagcctctgc acctgggtca actgatacgg
     9901 tgctttggcc gtgacaatgc tcgtaaagat tgccaccagg gcgaaggaag aaatgacttc
     9961 gcctcccgta tctggaaagg tgtacatggc ttcacaactg actgatgcat ttgcgcgtaa
    10021 gttttactac ttgcgcctgt cgattaccga tgtgtgtaac tttcgttgca cctactgcct
    10081 gccggatggc tacaaaccga gcggcgtcac caataaaggc tttcttaccg tcgatgaaat
    10141 tcgccgggtt acgcgcgcct tcgccagtct gggcaccgaa aaagtgcgcc tgacaggcgg
    10201 tgagccgtct ttacgccgcg actttaccga tatcatcgcc gctgtgcggg aaaacgacgc
    10261 tatccgccag attgcggtca ccaccaatgg ttaccgtctg gaacgcgatg tggcgaactg
    10321 gcgcgatgcg ggacttactg gcattaacgt cagtgtcgac agtctggacg cccgccagtt
    10381 tcacgctatt accgggcagg ataaattcaa ccaggtcatg gcggggattg atgctgcatt
    10441 tgaggccggt tttgagaagg tcaaagtcaa taccgtgctg atgcgtgatg ttaatcatca
    10501 ccagctcgac acctttctga actggatcca gcatcgccct atccagctgc gtttcatcga
    10561 actgatggaa acgggcgagg gcagcgagct cttccgtaag catcacatct ctggtcaggt
    10621 tctgcgtgac gagctactgc gtcgcggctg gatccaccaa ttacgtcaac gcagcgacgg
    10681 tcccgcgcaa gtcttttgcc atccagatta cgccggagag attggcctta tcatgccgta
    10741 tgaaaaagac ttctgcgcca cttgcaaccg cctgcgcgtt tcctccattg gtaaactcca
    10801 tctctgcctg tttggtgaag gcggcgttaa cctgcgcgat ctgctggaag acgataccca
    10861 gcaacaggcg ctggaagcgc gtatttcagc ggcgctgcgg gagaagaaac agacccattt
    10921 cctgcatcaa aacaacaccg gtattacgca aaacttatcg tacattggcg gctaaaacgt
    10981 caaaaggaga gatcagatga gtcaggtaag cactgaattt atcccgaccc gtattgctat
    11041 tcttacggtt tctaatcgtc gcggtgaaga agacgatacc tccggtcact atctgcgcga
    11101 ttcggcgcaa gaagcgggcc atcacgttgt cgataaagcc attgtgaaag aaaaccgcta
    11161 cgctattcgc gctcaggtat ctgcgtggat cgccagcgac gatgtacaag tggtattgat
    11221 tacgggtggt actggcctga cggaaggtga tcaggctccc gaagcattgc tgccgttgtt
    11281 cgaccgtgaa gttgaaggtt ttggtgaagt gttccggatg ttgtcttttg aagagattgg
    11341 cacttccacg ttgcaatctc gtgcggtagc gggcgtcgcc aacaaaacgc tgattttcgc
    11401 catgccgggt tcgaccaaag cgtgccgtac cgcatgggaa aatatcatcg cgccgcagct
    11461 ggatgcccgt acgcgtccgt gtaatttcca tccacatttg aagaaataag tatgtcgcaa
    11521 ctgacccata tcaacgccgc tggcgaagcg cacatggtgg atgtctccgc caaagcggaa
    11581 accgtgcgtg aagcgcgggc ggaagccttt gtcaccatgc gcagcgagac gctggcgatg
    11641 attattgatg gtcgccacca caaaggcgac gtatttgcca ctgcgcgtat tgccggtatt
    11701 caggcggcaa aacgcacctg ggatctgatc ccgctctgtc atccgctgat gctcagcaaa
    11761 gttgaagtca atttacaggc cgagccggag cacaatcggg tgcgtataga aaccttatgc
    11821 cgcctgaccg ggaaaaccgg tgtcgaaatg gaagcattaa ccgcggcctc cgtggcggcg
    11881 ctgaccattt atgacatgtg caaagcggtg caaaaagata tggtgattgg tccggtacgt
    11941 ttgctggcga agagcggcgg caagtcgggt gactttaagg tggaagcgga tgattaaagt
    12001 tctttttttc gcccaggtgc gcgagttggt gggaacagat gcaaccgaag tggctgcgga
    12061 tttcccaact gttgaagcgt tacgccagca catggctgcg cagagcgatc gctgggcgct
    12121 ggcgctggaa gatggcaaat tactggctgc cgtcaaccag acgctggtga gttttgacca
    12181 tccgctgact gacggcgacg aagtagcttt cttcccgccg gtaaccggag gttaagatgg
    12241 cagaaaccaa aattgttgtt ggtccgcagc cgttcagcgt aggagaagag tacccgtggc
    12301 tggcggagcg tgacgaagac ggtgcggtag tcacctttac tggtaaggtg cgcaaccata
    12361 acctgggcga cagcgtcaaa gcattaaccc tcgaacacta tccggggatg actgaaaaag
    12421 cactggcaga aattgttgat gaagcgcgta accgctggcc gctggggcgc gtcactgtga
    12481 ttcaccgcat cggggaatta tggccgggcg atgaaatcgt ttttgtcggt gtcaccagtg
    12541 cgcatcgcag cagtgcgttt gaagccgggc agtttattat ggattatctc aaaacccgcg
    12601 caccgttctg gaagcgcgaa gccacgccgg aaggcgaccg ctgggttgaa gcccgggaga
    12661 gcgatcagca ggcggcaaaa cgctggtagt tttttgttag ccggataagg caccgctgcg
    12721 tccggcaatt tcaccgctca gtacatacgt ttacgctgcg tcgatgcaca gcctcatcat
    12781 tttgcagtat ccttaagata ttccttatat cttcaggaga tcgtcatgga cagattccca
    12841 cgttctgatt caatcgtaca accccgggct ggcttgcaaa cttatatggc gcaagtctat
    12901 ggctggatga ccgttggctt gttgctgacc gcatttgttg cctggtatgc ggctaattcc
    12961 gcggccgtga tggagctgtt gttcactaac cgtgtctttt taatcggtct gatcatcgcg
    13021 caattagcat tggttattgt gttatcagcg atgattcaaa agctgagcgc aggtgtaacg
    13081 acgatgctct ttatgcttta ttcggcgctg acgggtctta cgctttccag tatattcatt
    13141 gtctataccg ctgcttctat cgccagtact ttcgtcgtta ctgccgggat gttcggcgca
    13201 atgagcctgt acggttacac cacgaagcgc gatttaagtg gctttggcaa tatgctgttt
    13261 atggcgttaa tcggcattgt gctggcatcg ctggtcaact tctggttgaa aagcgaagca
    13321 ttgatgtggg cagttaccta catcggcgtg attgtctttg tcggattgac ggcgtatgac
    13381 acgcagaaac tgaaaaatat gggtgagcag attgataccc gcgacacgtc gaacctgcgc
    13441 aaatattcca ttcttggcgc gttaaccttg tatctggact tcatcaacct gttcctgatg
    13501 ttgttgcgga tcttcggcaa ccgccgttaa attctcttct tcctgctgat cccggcctgg
    13561 agccgggatt tttttcggat tttataaaga ctattccgct catttacatg agaactgaat
    13621 cgcggcaaat gccagactat gtccactacg taatttcagg gacagataac gcatttgaac
    13681 agacaatcca ctgctactca attacagcaa aaaagtgctt cgctcagcaa aatgctgctt
    13741 ctcatttggt ggttaacagc agctggagta tgggctacgg caggctgtgc ctggatattt
    13801 cagcatttgc attgggttac gtcacaaaag tctccaggtg cgtttgtctt cgctttggct
    13861 gcgctgataa taggtggtct ctggttaaac aacgccctga aaagactgaa tgtatcaggc
    13921 gcatgtctgg tctatctgct aataatcctt cttgttggct ttctcttcgc cagcctttat
    13981 accttttcga gcatagcgac ggtaatggga attacgggag ggatgtttgc cgcaatggcg
    14041 ctgatttgct cttgtagtaa tagggttatc ccaccagtca gacagttata ttcatatatt
    14101 ttctgtggtt tgagtattgc ttttgttgtt aacctgatcc ttaccagcag tttttccgtg
    14161 tggttagcga gtattttgac tgtctttatc tggggcatta cggctgcctg tgaagcaaca
    14221 acactggaag atttgatccg tgttgccgac acctctgaaa tttcaggttc tcttcgctgt
    14281 atcgttcctg gggcaataac actctatttt agtattttaa gcgtcctctt tagaattact
    14341 gtaacagttc ttgaatttat taatggttta gtgtggtagt gcgaattgac gagttttatt
    14401 gcgttaattg gaatagataa tgatatttaa cttaaggata taaaaataat gaattcgcgc
    14461 tcagcaaata acataactaa tgattttcag cacgaagcca ggatattaaa cagcatatgg
    14521 ctcattaccg ctttaggttt ggtgacaacc gcaggactgg cctgggtagc gaagtatctc
    14581 gaaattacgg caacaaaata tgattcaccg caaatgtatg tcgccatagg attattatta
    14641 ctttgtatgt atggcttaag taaggatatc aacaagataa atgccgtcat cgcaggcgta
    14701 atatatctat ttttactctc tttggtggcg attgtcgttg ctagtttagc tcctgtacct
    14761 gccattatcg tcgtgttcag cactgcgggc gcgatgtttt taatcagtat gctggccggt
    14821 ttattattta atgttgatcc tggttctcac cgttttatca ttatgatgac gttgacaggg
    14881 ttggccctgg tgatcatcat gaatgccgca ttaatgagtg aacgacccgt ttgggtaata
    14941 agttgcttaa tgattgtgtt atggtcaggc attatctcgc atggacgaaa taagctcctt
    15001 gaattggcgg ggaaatgcca tagtgaagag ttgtggagtc cggttcgttg cgctgttaca
    15061 ggtgcattaa cactctatta ctattttatc ggcttctttg ggatacttgc cgcgatagct
    15121 ataacgcttg tctggcaaag gcatacgcgt ttttttcatt agtcgtaaat gcagatacta
    15181 aagaacttaa ctaaacttca catcgccgct tcatttttcg cccgtagctt cttcgcctgg
    15241 ctttccagta gcaggtaaca gatcagcgcc agcagcagcg ggataaagta atacaggacg
    15301 cgataagcga gcagggcggc gataatgctc cctttcgagg tatgctcccc agccagtagt
    15361 gcgataaaca ccgcttccag cacaccgatc cccgccggaa tatgcacgat gacgccagca
    15421 atactactaa ccagtaacac gcccagtaca aagaaatagt tcacgctttg accaagtaac
    15481 agccagataa tcgcccccat taccatccag ttaacgctgg aaatcagcat ctgagcgagg
    15541 gcgaatttcc atgaaggcag caccagcttt tgccctttga tggtcatatg gcggtgcttc
    15601 gcgaaagcgc aaaaccacaa ataaaccgcg ataatcatca gtaagccaat gccgagaata
    15661 cgcagcgtag tttgatcgac ataccagtga tccggcaact ccaccacgcc tgcggtaaag
    15721 ataatccctg ccagtaaaat atagcccagc cagttggtgg taatactgag cgagaaaatc
    15781 cgcgtaatag tgctgcccgg taaccctagt cgggagtaca aacgatagcg cataccaatg
    15841 ccgccgaccc aggtactgag cgtcaggttg aaggcgtagc agataaacga caccagcatc
    15901 acctggcgct tcgccagttt gtgaccgcag taaaagcggg cgagcaggtc atagcagccg
    15961 taaatcagat agctgacgac caccagcccg accgcactaa gcagcgcaac gcgattgtag
    16021 tcgcggatga ccttccagac ctcttcccag tccacttttt tggcgtagac caccagcaac
    16081 acaatcaccg cgataaaaaa cagccaggtg aggatcttct ttgctaagcg ccagcgcggg
    16141 tgtgatttac tcatcagggt tttacccccg tgttttcagt ttctacccga tcctgcgttt
    16201 ccattgtcgg ttgtgcgggc ggatcaacct gcgccagacg tggtgtgtgt gccggaagcc
    16261 agccaaccag cgccgggaag tggcgtaaaa agtggaacgc cagcacgctt ttggtcaggt
    16321 tccaccaggt gcgtttcggc agcatggtct catcgacctg ctgacaatct gcggcaataa
    16381 tgccgttcag attatcgcgc agcgtctggt taaaatgacg atagtggatg atgacatttg
    16441 cttcgagatt cagtgacaaa ctgagcggat cgagattact ggaccctact gtcgcccagt
    16501 gatcgtccat caatgccact ttgccgtgga gcgggcggcg gcggtactca aatacctgaa
    16561 cgccgccttt aaccagatag ttatacagca agcgcgcacc gactctgaca atcggcatat
    16621 ccggttcgcc ctgaatgatc agtttgatcc gcaccccgcg ccgtgccgct ttacgcaagg
    16681 cgtgtaaaaa tcgatagccg gggaagaagt aggcgttggc gataatgact tctcgccgcg
    16741 cctgagtgag cattttcaga tagtgacgtt caatatcatc gcgatgttct tcgttatcgc
    16801 gccagaccag caatacctgc gcttctcccg gctggcggtt ctcttccgct ttgtgatgac
    16861 gtcgccacca gcgtcgtgcc gcgctctgtc caggcaggtt ttccagctca aactggagaa
    16921 tatcttcgac aatcggccct tcaaggcgta ccgcgtaatc ctgtttagcc tctggaccgt
    16981 agctggacat atgctcggcg gagtaattca gcccgccaat aaaggcgata cgcgcgtcga
    17041 tcaccacaat tttgcgatgc atccggcgaa acacattggt gcgcatacca aaaaggcgag
    17101 ggcggggatc gtagtagcga aataccacgc cagctgccgt cagttcattg acaaactcat
    17161 cgctgagatc cggcgaaccg tagccatcca gcaagacttc cgctttaacc ccgcgttgcg
    17221 ctgctgccag tagtgccgca tgcagctgtt tgccgacgtc atcctcaaac cagataaacg
    17281 tttcaagaat gatgcgttct tgtgcctcgc caatcgcctt aaacaccgcg ggataatatt
    17341 gctcgccgtt ttccagcaac tggatcttat tgccttcgcg ccagctacat ttcataaatg
    17401 aatctccgca cttaaagggg catgatcaga aaggtgtcgc catgtccgca gcggcaacgc
    17461 ggttggcgcg ctggcgctgg catttttgac gtagatcctg tccagtcgta gtagaggaaa
    17521 ttgcaccgga aacgtgcgcg ccgggcgtcc gtgggcgcgg gtaaaaatct catccagtcc
    17581 ggcctgcact tttaacggat gattagcttt ttgccgccag tcattgaaat cacccgccac
    17641 caataccggt tcgccgtccg gtagctcatt cacccattcg gcgagcatcg caagctgcgc
    17701 ctgacggtgc gcctcacgca ggcccagatg tacgcacatc acatgaatcg cttttccggt
    17761 catcggtggc acaatgcggc agtagagcac gccgcgcttt tccgcaccat cgaccgaaac
    17821 atcgcgattc tcataatgtt caatgggata acgcgacagt acggcgttgc cgtgatgccc
    17881 ttccgggtat acggcattgc gaccgtaggc aaaatcgctc cacatcgtgt cggcgagaaa
    17941 ctcgtagtgc gaggtatcgg gccagttttc cacatgcagc ggatgaactt cgtgcgcgcc
    18001 catcacttcc tgcaggcaaa caatatcggc gctgacggta cgcaccgcgt cgcgaagttc
    18061 cggcaaaatg aagcgtcggt taaacgcggt aaaacctttg tgaatgttga tggtgagcac
    18121 cttgaacgaa aattgttgtg tttgatcggg cataattttc ctgtctttgc ctcttatctc
    18181 attgaaatag tgtagtcggc gtcacaaaaa ggtgcggtct tacggaattt tccgtaaagt
    18241 tcggtactct gagtaagtag agataaattc ttcaggagag aagccatgaa gtggcaacaa
    18301 cgtgttcgtg tcgcaactgg tctaagttgc tggcagatta tgttgcattt actggtagtg
    18361 gcgctgctgg tggtgggctg gatgagtaag actctggttc acgtcggcgt gggattatgc
    18421 gcactgtatt gtgtcacggt agtgatgatg ttggtgtttc agcgccaccc cgagcaacgc
    18481 tggcgtgagg tggcagacgt gctggaagag ctgaccacga cctggtattt tggcgcagcg
    18541 ctgattgtgc tgtggctgtt gtcccgtgta ctggaaaaca actttttgct ggcaattgca
    18601 gggctggcaa tccttgccgg cccggcggta gtgtctttgc tggcgaaaga taagaagtta
    18661 catcacctta cgtctaaaca tcgcgtacgc cgctgaccct gtcgtggccg ttatcaccag
    18721 tagcggccac aaactattcc acacaatatc cagactcgca tccttcaaat aaatctgctt
    18781 ggtaatgtcc gtaaagtggc gaatagggtt aatccacgtc aggttttgca gccatactgg
    18841 catgttttcc accggagaaa cgtaaccgga aaggagaatg gcgggcatca taaaaacaaa
    18901 cacgccgata aacgcctgct gttgtgtcga gcagagtgat gaaatcaaca gaccgaatcc
    18961 caccagcgat aaaccgtaaa tcaccatcgt aaagtagaac agcgccagcg atccggcgaa
    19021 ggggatttga tacgcccaga taccaatcgc cagcacaatg gtggcctgga aggtggcgac
    19081 aattaacgcc ggtacggctt tgccgatgaa gatctgccag gtggtgagcg gcgaaaccag
    19141 tagctgatcg agcgtacctt gttcacgttc gcgggcgacg gaaagtgaag tgacgatcat
    19201 tacgccgata gtggtgatca tggcgatcag cgacggcacc acaaaccatt tgtagtcaag
    19261 attcgggtta taccagttgc gtaccaccag ctcgctgttg ttaggtttcg gttttccttc
    19321 cagcagctcc tgctgataat ttttgacgat ctgttgcagg tagttggcgg caatttgcgc
    19381 actgttggag ttacgcccgt cgaggatcaa ctgcaacggc gcggtctgga aggtatccag
    19441 tttgcgcgag aagtcagccg ggaaacgcac cagtagtaac gccttttgtg tgtcgatggt
    19501 tgggcggatc tcctgtgggc ttttcagcag cagcacatga gtaaaggcgc tggcgcgggc
    19561 aaaacgttgg gtcagctcca ccgaatgctc gccgttatct tcatcgtaga tggcgatggt
    19621 ggcgttagtc acttccagcg tggcggcgaa cgggaacaga atcacctgaa taagcacagg
    19681 taaaatcaga atcgcgcggg tttgcggttc gcgcagcagc gactgcaact ctttgcggat
    19741 taacgtccat aagcgatgaa acatgctctt ctccctaatc cagccgacgt ttggttttca
    19801 gccacgtcag gccgataaac atcaccgccg aagcgatcaa aaacagcacg tttaccacca
    19861 gcaccactgg aatattcccg gcgaggaaca ggctttgcag ggtgctgacg aaataacgag
    19921 cgggaataat gtacgtcacc gcgcggatca ccgcgggcat actgtcgatc tgaaaaataa
    19981 agccggaaag cataatcgac ggcagaaaag cggcgttcag ggcgacctga gcggcattga
    20041 actggttgcg ggtaatcgtg gaaatcagca gccccatccc cagggtactg agtaaaaaca
    20101 ggctggagat aaaaaacaga atcagcagcg acccgcgata cggcacgccg agaataaaca
    20161 ctgacaccag catacacagc aacatcgcca gcatcccgag aaagtaataa gggatcagct
    20221 tacacagcag cagttccgtg cgggtaatct ccgtagagag cagagcctcc atggtgccgc
    20281 gttcccattc tcgcgccacc accagcgagg tgagaatcgc gccgatgacc gtcatgataa
    20341 tggtcaccgc accggggata atgaagtgct ggctaatcgc cgccgggtta aaccagtagc
    20401 gggtttgtac atcaataagc ggttcaaaag tctgcccgtt gtcctccgct cgctgcattt
    20461 gccagatctg ccagatccct tcgacatacc cctgtacaaa gttagcggta ttcggctcac
    20521 tgccgtcggt gatcacctga atcggtgcgg tggcgttggc gcgctccatc tgttccgcaa
    20581 aatccaccgg aataaccacc agaccgcgaa ttttccccgc ctgcattttg gcgatcagtt
    20641 cctgacggtt atcgctgatg gtggcgtcga tgtagggcga accggtcatg gtgtgggtga
    20701 aatccagcgc cgcttcgcta cgctgttcca gtaaaatccc gacccgcagc ttgctggagt
    20761 cgaggttaat gccgtaacca aaaataaaca gcagcaacag cgggatcact accgcaatca
    20821 gccagctact cggatcgcga acgatctgcc gcgtctcttt gacgcacagc gcccgcacgc
    20881 gacgccagga aaggatcggg ttactcattg ctatgctcct tatcccagtc gtggatcaac
    20941 tgaataaagg cttgctccat ggtgggatcg ggttgctcat cgttagccga ctgtgctttc
    21001 aaatcgtccg gcgtgccgct ggcgattaat ttcccgcggt acaccaggcc gatgcggtcg
    21061 caatattccg cttcatccat aaagtgggtg gtgaccatca ccgtgacgcc tttctctacc
    21121 atgctgttga tgtgcagcca aaattcacgg cgggtgaggg ggtcaacgcc ggaagtcggt
    21181 tcgtcgagaa acagaatatc cggttcatgc atcagcgaac aggccagcgc cagccgctgt
    21241 ttaaaaccta atggcagttc atcggtggcg tgggaggcaa tacttttcag gccaaatgcc
    21301 tcgctcatac gggagatttt ttcgttctgc gcccgaccgc gtaagccata cacaccagag
    21361 aaaaagcgta aattctgttc gaccgtcagg ttgccataga gcgaaaactt ttgcgccata
    21421 tagccgagat gctggcgcgc tttaccggaa ctctctttca gatccatccc cagcaccagc
    21481 gcctgaccgg aggtcggcac cagcaagccg cacatcatct taaaggtggt cgatttcccc
    21541 gcgccgtttg gcccgagcag accaaaaatc tcccctcgtt taacggcaaa gttgacgtga
    21601 tcggtggcgg caaaatcacc aaatttcttg gttagttctt tcgcttcgat caccgtctcg
    21661 ccgggtgtgc cttctaccgt atgcaatatt gcacccagcg gcgattccga ggttccggca
    21721 ccgcccagca aatcaataaa cgcatcttca aaacgcggcg tagtttcgtt gatgttgatt
    21781 tccggcatcc cttcggcatg gcgaatatcg tctggtgtgg cctctttttt gaggatcaga
    21841 cgtaccgatt tcccctgaat catgccgtcg ctgacctgtg gcagtttcaa ggcgcgttgc
    21901 aatagtttgc ggttgccctc gtgtggactg gtcatcagaa aactgcgtcc ggccatggtt
    21961 tgtgtcaggg cttttggttc cccctgatac agcaactcgc cttcgttcat cagtaacacg
    22021 tcacggcact gctcggcttc gtcgagatac gaggtactcc agaggattaa catcccttcg
    22081 cctgccagct catgcaccat ctgccacagt tcgcgccgtg agatagggtc aacgccgacg
    22141 ccgggttcat cgagcagcaa cactttcggt tcgcccacca gggtacaggc cagaccgagt
    22201 ttttgtttca tcccaccgga gagcttgccc gccaggcgtc cggtaaacgg cccaagagac
    22261 gtaaactcca gcaggcgagc aaaagtttgc ttacgtgcct cgccggtgac gctgcgcaaa
    22321 tccgcgtaca gattgaggtt ctccatcacc gtgagatctt catacagacc aaatttctgc
    22381 ggcatatagc cgagcacggc gtgcagcgcg ccgtcgtttt tgattggatc aaagccaatc
    22441 accgtggcat tgccgctgtc gggtttcagt aatcccgcca gcatccgcat tagcgtggtt
    22501 ttacctgcac catccggccc caccagcccc gtcacataac cggcgtgaat ggtacaatcg
    22561 agcggcgcga cggcgggctt gtccatgccc ggaaagcgtt tttctaggcc gttcagcgtg
    22621 ataacggcat cattcatgtc ctgcctcgtt accgaattgc accgtcaccg gcattccctg
    22681 gcgtaacgca tcatcggcgt cggtcaccac aatacgcagg cgatagacga ggtcggtacg
    22741 cagatccggt gtttcgacgg ttttcggggt aaattcagca gtcggcgaaa cgaaaccaat
    22801 ctgtccgtga tagggcttgt ccgggcgacc atcggtataa agcagcactt tgcgccccgg
    22861 ctgggcctgg tcaagattcc gttcatcaac ataggcacgt acccacaccg gacgcgttag
    22921 tgaaacggta aacaccgtgc caccttcatt gagaaccgtg cctggctcca ccgcgcgcgt
    22981 taacagcgtg ccatcagacg gtgctatcaa cgttgagtcc tgtaaattca gctccgcctg
    23041 cgccagttgc gcctgcgcct gttcgaggct ggctttcgcc tgagcgatgt cctgttcacg
    23101 gttaccggaa cggtactgac gcaatttatc ctgtgctgat ttcagcgttg cctgtgcctg
    23161 gtcgcgcgag gagcgggcat tttccaggtc atttgccgaa atggtgcggc ttttccacag
    23221 tccttgctgg cggttataga agttctgcgc atagtcatag gcggcttgag cctgtttcac
    23281 cgctgcggcg gcctgagcga tttcttcatc gcgatacccg gcaagcatca gatcatactg
    23341 cgcctgtgcc accgagacgc ccgctttcgc ctgcatcagg gcaatctcat acggcttgtg
    23401 atccagttcg cccagcacct ggcccgcttt gatagcatca ccttcgtcca ccgccagcga
    23461 ttcaacgcgc cccccaacac ggaaactaag atttaccgta cgaatatcca cgttgccata
    23521 cagcgtcagg ccgttatcct ggcggctttg ataccaccag tagcctccgg caaccacggc
    23581 ggcaagtacc actaccgcca atccgatcac gacaggtttt ttcatcacta caaactcctt
    23641 tgcgataatc cttgcaaaat caggtcgata tgacaggtca ccgtctggtt gatcagttcg
    23701 gtcttttctt catcgaacgc ggtccagccg gtacgtaaca gaattgtttc tttgccaaga
    23761 cggaacgcca gaatctcacc aatcaacgca tgagtatgga ggatcatgcg ggtatcgttg
    23821 gcgtcgcagc cggtccaggc ggcaatcagg cgtgtcaggt ggctgtgtag cgggctaatc
    23881 acctgttcgt gcaccaggtg ataggctgcc gtgggagaga gctgctcacg ggagataaac
    23941 ttgctgaggt tgacggtgtc atcctgggtg agcagcttaa tcatgttcct gcaggcgcga
    24001 aggatcagtt cacggatggc tgcgcgatca ggctgtggtt gtgcgaacaa gcgttcggcc
    24061 tcttcggcgt gcggacggaa ctgctcgcca ataaaatcgg caatccactg ggcgcaggcg
    24121 aggtacaaat cttctttcga accgaagtag taggtgatgg cagcgatatt ctgcccggcc
    24181 tgggcggcta tctcgcgggt ggtggcgttc attccatatt caccaaactg cgccagtgcg
    24241 gcagcaatca gctgtttttt cgcctgttca cccttgattg tcatggcagg attattcatc
    24301 gcacagtcca ttcttaatca aatgattgat taagattatg actccatatg ggagttgtcc
    24361 agtatggcta agaattttag ctacgccagt cacagggata atttatgcgc tgcgtcacaa
    24421 aaactgctac actccgctcc ctcatgacat tgtggttttt gtcattttcc ttttcagtat
    24481 ctccctgaaa actacaccgg taacggtcgg ggcggttcgg agtagttatg tctttcgatt
    24541 ctttgggttt aagccctgat atcctgcgcg ccgttgccga gcagggttac cgtgaaccca
    24601 cccctattca gcagcaggcg atccctgcgg tgctggaagg ccgcgacctg atggctagcg
    24661 cccagaccgg taccggtaaa acagcgggct ttacgctgcc gctgttgcaa cacctgatca
    24721 ctcgccagcc gcacgccaaa gggcgtcgtc cggtacgtgc gctcattctt accccgaccc
    24781 gtgaactggc tgcgcagatt ggcgaaaacg tccgtgatta cagcaaatat ttgaacattc
    24841 gttcgctggt ggtgtttggc ggtgtcagca ttaacccgca aatgatgaag ctgcgcggcg
    24901 gcgttgatgt tctggtggca acaccgggac gtttgctgga cctggaacat caaaatgcag
    24961 tgaagctgga tcaggttgaa atcctcgtcc tcgatgaagc tgaccgcatg ctcgacatgg
    25021 gctttatcca cgatatccgt cgcgtgttaa caaaactgcc agcgaaacgc cagaacctgc
    25081 tgttctctgc gaccttctct gacgatatta aagccctggc ggaaaaactg ttgcacaacc
    25141 cgctggaaat cgaagtggca cgccgcaata ccgcgtctga tcaggtgacc cagcacgttc
    25201 actttgttga taagaaacgc aaacgcgaat tgctgtcgca catgattggt aaagggaact
    25261 ggcagcaggt gctggtgttt acccgtacca aacacggcgc taaccatctg gctgagcagc
    25321 tcaataaaga tggcatccgt agtgcggcga tccacgggaa taaatcgcaa ggtgcgcgta
    25381 cccgtgcgct ggctgatttt aaatcgggtg acattcgcgt actggtggcg accgatatcg
    25441 ctgcgcgcgg tctggatatc gaagagttgc cgcacgtcgt caactatgaa ctgccaaacg
    25501 taccggaaga ttatgtccac cgtatcgggc gtaccggtcg tgcggctgct accggtgaag
    25561 cgttgtcgct ggtgtgtgtt gatgaacaca aactgctgcg tgatatcgaa aaactgctga
    25621 aaaaagagat cccgcgcatt gcgattccgg gctatgagcc ggacccgtca atcaaagccg
    25681 aaccgatcca gaacggtcgc cagcaacgtg gcggcggcgg tcgtgggcaa ggtggtggtc
    25741 gcggtcaaca gcaaccacgc cgtggggaag gtggcgcaaa atctgcaagc gcgaaacctg
    25801 cagaaaaacc gtctcgccgc ctcggcgatg ccaaaccggc aggcgaacaa caacgtcgcc
    25861 gccgtccgcg taaacctacc gctgcgcagt aatcttttat gccgggctat gcccggcatc
    25921 aggctgatga acaaacgcaa aactgcctga tgcgctacgc ttatcaggcc tacgtgaact
    25981 ctgcaatata ttgaatttgc atgcttttgt aggccggata aggcgtttac gccgcatccg
    26041 gcatttcaca acaagcactt gtcagcaatt tgagaacacg ggaaaaaatt tatctgtttt
    26101 accgcccata agccaccgag acgttacttc tctatagcca attgttcgcg caactccatt
    26161 aaaaggtagc ccagtcgatt cttgccctta ccattaccac cgtctcccca gtaagcatcg
    26221 ttttctgtat gctcaaccag ttttgcgggc gcggttgcca gcaagagcgc acgcagttct
    26281 ggatgctgtt cgaatttagc gcgaagtgct tttcgcatca cctgttcttt gaccgactcc
    26341 cagtttttac gcagaggctt agaacgatcg cgccccatgc gtgcggcgac cataggggaa
    26401 gaaacccgac ggatctcttc gcggtatttt tcatcaagga atttttgtgc ctgaaaatag
    26461 tgttctgagg tgggccaggt tttcccgtca accttgatgg gccaggcggc aaagttagaa
    26521 aaatcaccgt agtcatcgct ggtgctgtag aaatttatga tggtgtcttg catgacgtgt
    26581 tggatccttt gtgctcgaac gggcattaaa ccgcattatg ttggtggtta ttgcgagccg
    26641 ctttccagaa acagaaaaac cattacccct gaaaaccgaa aaatgccaca atattggctg
    26701 tttatacagt atttcaggtt ttctcatggc attaaccgcc gcgcttaaag cgcaaattgc
    26761 cgcctggtat aaggcgcttc aggaacagat ccccgacttt attccccgtg cgccgcagcg
    26821 gcagatgatt gcggacgtcg ccaaaacgct ggccggagaa gaagggcggc atctggcgat
    26881 tgaagccccc accggcgttg ggaaaacgct ctcctatttg atccccggca tcgccattgc
    26941 ccgcgaagag caaaaaacgc tggtggtgag taccgccaac gtggcgttgc aggatcagat
    27001 ttacagcaaa gatttaccgc tgctgaaaaa gatcattccc gatcttaaat tcactgccgc
    27061 ttttgggcgt gggcgctacg tttgcccgcg taatctgacg gcgctcgcca gcactgagcc
    27121 cacgcaacag gatctgctgg cgtttcttga cgacgaactg acgccaaata atcaggaaga
    27181 gcaaaaacgt tgtgcgaagc tgaagggcga tctcgacact tataaatggg atggtctgcg
    27241 cgatcatact gatatcgcta tagatgacga tctctggcgt cgtttaagta ccgacaaagc
    27301 cagctgcctc aaccgcaact gttactacta tcgtgaatgc ccgttttttg ttgctcgtcg
    27361 ggagattcag gaagcggaag tggtggtggc aaaccatgcg ctggtgatgg cggcgatgga
    27421 aagcgaagcc gtactacctg acccgaaaaa tctgctgctg gtgctggacg aaggtcatca
    27481 cctgccagat gtggcccggg atgcgctgga gatgagtgcc gaaatcaccg cgccctggta
    27541 tcgactacag ctggacttgt tcacgaaact ggtcgctacc tgcatggagc agtttcgccc
    27601 gaagaccatc ccaccgctgg cgatccctga acgtttgaat gcgcattgtg aagagttgta
    27661 tgagcttatc gcctcgttaa acaacattct caatctctac atgcctgccg ggcaggaggc
    27721 agagcaccgt tttgcgatgg gcgaactgcc tgatgaagtg ctggagatct gccagcggct
    27781 ggcaaaactc accgagatgc tgcgtggtct ggcggagtta tttcttaacg atttaagtga
    27841 gaaaaccggc agccacgata ttgtgcgtct gcatcggttg attttgcaga tgaaccgcgc
    27901 gttggggatg ttcgaggcgc aaagcaaact ctggcggctg gcgtctctgg cgcaatcttc
    27961 cggtgcgccg gtgaccaaat gggcgacgcg ggaagagcgc gaagggcagc tgcatctctg
    28021 gtttcactgc gtggggatac gtgtcagcga tcagctggaa aggctgctgt ggcgcagtat
    28081 tccgcacatt attgttacct ccgcaacctt gcgttcgctg aatagttttt cgcgtttgca
    28141 ggagatgagt ggtctgaaag agaaagcggg cgaccgtttt gtggcgctgg attccccctt
    28201 taaccactgc gaacagggca aaattgttat tccccggatg cgcgttgagc cttccatcga
    28261 caacgaagag cagcatattg ccgaaatggc ggcctttttc cgtaagcagg tggagagcaa
    28321 aaaacatctc ggtatgttgg tactgtttgc cagcggacgg gcgatgcagc gctttctcga
    28381 ctatgtgacg gatttacgcc tgatgctgct ggtgcagggc gatcagccgc gttatcgctt
    28441 agttgaactg caccgcaaac gcgttgccaa cggtgagcgt agtgtgctgg tgggcttaca
    28501 gtcatttgcc gaagggcttg atttgaaagg tgatctgctc agccaggtgc atatccacaa
    28561 aatcgctttt ccgcccatcg acagcccggt ggtgatcacc gaaggggaat ggctgaaaag
    28621 cctcaaccgc tatccgtttg aggtgcaaag cctgccgagc gcctcgttta acctgattca
    28681 gcaggttggg cgactgattc gaagccacgg ttgctggggc gaagtggtta tctacgacaa
    28741 acgcttgctg accaaaaatt atggcaagcg actactggat gcattaccgg tatttccgat
    28801 agagcaaccg gaagtccctg aaggtatagt taaaaagaaa gaaaaaacga aatccccacg
    28861 ccgtcggcgg cgttaatgat gtgagtcagg taaggagtcg taaatggact atcgcaaaat
    28921 cattaaagag atcgggcgcg ggaaaaacca cgcgcgcgat ttagaccggg atactgcgcg
    28981 cggtctgtat gcccatatgc tcaatggtga agtccctgac cttgagttgg gcggcgtatt
    29041 gattgcgcta cgtatcaaag gggaagggga agcagagatg ctcggctttt acgaagccat
    29101 gcaaaatcac accatcaagc tgacgccgcc agcaggcaag ccgatgccga ttgtcatccc
    29161 cagttacaac ggcgcgcgta aacaggccaa cctgacgccg ttgttggcga ttctcctgca
    29221 caaactcggt tttcctgtgg tggttcacgg ggttagcgaa gatccaaccc gcgtgctgac
    29281 tgaaaccatt tttgaattga tgggtattac gccaacgttg cacggcgggc aggcgcaggc
    29341 gaagctcgac gaacatcaac cggtgtttat gccagtcggc gcgttttgcc cgccgctgga
    29401 aaaacaactg gcgatgcgct ggcggatggg cgtgcgtaac agtgcacata ccctggcgaa
    29461 actggcgacg ccatttgccg aaggtgaggc gctgcgtctt tccagcgttt cgcatccgga
    29521 atacattgga cgcgtcgcga agttctttag cgatatcggc gggcgggcgc tactaatgca
    29581 tggtactgaa ggtgaagtgt atgctaatcc gcagcgctgc ccgcagatca atctcattga
    29641 ccgtgaaggg atgcgggtgc tgtatgaaaa acaggaccct gctggtagcg agttactgcc
    29701 acaagcaaaa gatccggaaa ccacggcgca gtggattgag cgttgccttg ctggcagcga
    29761 accgattccc gaatcgctga aaatccagat ggcttgctgc ctggtggcta cgggtgaagc
    29821 ggcaactatc agcgacggcc tggcgcgcgt taatcaggca ttttaattct tttctcagcc
    29881 ggatgagcca tgtcatccgg cttttacccc accgcaatat gaaattcctg catctttatt
    29941 gaccttcccg cgcccggcgt gcagcataaa aaaacaacaa acacataaca taaacaggag
    30001 ttaaccatgg aaagtggtca tcgctttgat gctcagacgc tgcacagttt tattcaggct
    30061 gtatttcgtc agatgggtag cgaggaacaa gaagcgaaat tagtggccga tcatttaatc
    30121 gcggcaaacc tggcagggca tgattctcat ggtattggca tgatcccaag ctatgtgcgt
    30181 tcctggagtc aggggcatct gcaaattaac catcatgcca aaatcgttaa agaggcgggg
    30241 gcggcagtca cgctcgatgg cgatcgcgca ttcggtcagg tcgcggcaca cgaagcgatg
    30301 gcgctgggga ttgagaaagc gcatcagcac ggtattgctg ccgtggcgct acataactcg
    30361 catcatatcg gccgtatcgg ttactgggcg gagcagtgtg cagcggcggg gtttgtctct
    30421 atccactttg ttagcgtggt cggtattcca atggtcgcac cgttccacgg tcgcgacagc
    30481 cgctttggca ccaatccgtt ctgtgtggtt ttccctcgta aagataattt cccgctgttg
    30541 cttgattacg ccaccagcgc cattgcattt ggcaaaaccc gcgtcgcctg gcataaaggc
    30601 gtccccgtgc cgccaggttg cctgattgac gttaacggcg tgccgacgac caatccggcg
    30661 gtaatgcagg agtcgccgtt gggttcgctg ttgacctttg ccgaacataa aggctacgcc
    30721 cttgcagcga tgtgtgaaat tcttggcggg gcgctttccg gcggtaaaac gacgcatcag
    30781 gaaacgttac aaaccagtcc cgatgccatt cttaactgca tgaccactat catcatcaac
    30841 ccggaactct tcggcgcgcc ggattgtagc gcgcagaccg aagcctttgc cgagtgggtg
    30901 aaagcctcgc cgcatgatga tgataagccg attttgctac cgggcgagtg ggaagtgaac
    30961 acgcgtcgcg aacggcagga gcaggggatt ccattggatg cgggaagctg gcaggccatt
    31021 tgtgatgcgg cgcggcagat tggtatgtcg gaagagacgt tgcaggcttt ctgtcagcag
    31081 ttagccagct aaaaaaaagc ccgtccagtg gcggacgggc aaacaagggt aacataggat
    31141 caatgagggt tagagcatat gcgtctgtcg gcaaacagac agggaaatac ttgtgctgga
    31201 cgtagcgtaa acgcctgatc cgtccaacgg ttcggatttt gtaggcctga taagacgcgg
    31261 tagcgtcgca tcaggcatct gttgccggat gcggcgtaaa cgccttatcc ttcctacgaa
    31321 ttacttataa attaccgcag taccgcttaa tttattgttg ttggtggcgg aagtgatgct
    31381 gtatccactg gcaccggctg ccgcggcttt ctcagccagt ttcgcttcca gggcatcgag
    31441 ggtggatgcg ccatcggcag aaaccacgcc gattttattc atgttctgtg cctgggatgc
    31501 cgttaccggt tccgcggcaa acacgccaaa tgacagagtt gaaagagcca tagcagcaac
    31561 aacagtattg atagttttca taattaatct ctcgcaggtg atttttgtat aagtgacgtt
    31621 gtttcgtcga tgtgatgagt atcacgtttt tttacgagag ataaaatcga agagaattga
    31681 ccgccttgtt caaataaatt gattgatatc taacatactg ataaaccatt tatttgttgt
    31741 acttatggac ttgttttatg cagcgtggca gtcacgacag cgttaaaggc acggaatgcc
    31801 acgcaaaata caaattatac tcgctataca tctcgccagt tgtagttaac gtaactggct
    31861 gtctttcgaa cctctgcgat tatatcctgt atacgctggt ttttgtaaat ctttctcctg
    31921 ctgacaatga atgcataagc gcacgccagg aatggcttcc cgacgggctt gcgggatggg
    31981 ggcaccgcac tcttcacatt catccaggct ttcgccacgc ggaatttcac cccgagcgcg
    32041 ggcaatcgca tcttcaattg tactgttgat ctgttcgttg acggcgtcat cgttagccca
    32101 accggatgcc atatcgtcct ccccatatca atgcttgtac agttaagtgt agctaatcca
    32161 gggacgaact tgggcagttc aagcatcaga tctccgacca ttcccgcagc agattatgat
    32221 aaagattaag cagcgacagg atctcttcat tttcaccata gcggcttttc agcgactgaa
    32281 tattgttgtc cagttcaaac agcatggcgc gctttttatc atcacggatc atcgactgga
    32341 tccacataaa tgatgccact cgtacgccgc gggttacggg tgtcacgcaa tgcaggctgc
    32401 tggagggata caacacgaga tcgcctgccg ggagttttac ccgatgttgt ccgaaggtgt
    32461 cattaacgac cagttcgccg ccgtcgtagc tttgtggatc gcttaaaaac agcgtggcag
    32521 aaaggtcagt acgcatccag ccgttttgtg gatggctgcg tactgcgcca tccacatgga
    32581 aaccataggt ttcattgttc tgatagcgat taaacagcgg cgtggaaagg gtacgcggca
    32641 aggccgcggc aaagaataaa gcatgttggt taaccgcgtt cagcacctca ttttgcaggg
    32701 cggcgtataa cgtgctgcgg gtgtcgacct gttgattgtt cttaacttgc gcaccttgtg
    32761 cgccggtggt gacgcgtcca tccacccatt cggcttgttc cagttgttcg cgaaaccggg
    32821 cgacgtcctg tggcgataac acgccgggaa tgtggtacat catacaagtt ctccaaaaag
    32881 tggggcctgc gccccacatc tgaatcagaa atgcatattg gctgtgagca agaaggttct
    32941 tggctcgccc gggtgataac ggtagccgct cttgttgatt gaggcgacgt aatcggtatc
    33001 aaacaggttg taaacgttta gctggaagtc gagattgcga ttaacccgat accccagttt
    33061 ggcatcggcg acccagtaac cttcggtaaa cgctggcgtt cccaccgcgc cgtctgaacc
    33121 tttatgcata ctgccgatat agcgtgcgcc cgcgccaaca gagatatcat ctgttgcctg
    33181 atactggctc cataaggtga aggcgtgctc cggggtatac ggcagcgatg aggaaccatc
    33241 ctgggcaaca tctttgccgt ttttgatggt tgctttttgc tgggtatagc cgccaatcac
    33301 ctgccacgcg ggcgtgatat tcccggccac ggatatctca tagccttcga cacgtttctt
    33361 accgtattgc gagtaagttc cgtcatcatt ttgctcaact tcattttcga tatcagtacg
    33421 gaatagcgcg gcggtgagca acaggcgttt atccagaacc tgccatttgg tgccaatctc
    33481 gctggtgttg gctttttgcg gtttaaaatc ggtgcggttg gcactgttac cgctgccaga
    33541 ctgcgcaagg gcgaagttgt tgccgcccgg aggctgctgg gaaacggcat agttaatata
    33601 gacattaccg ttttccgtca ggtgatacag cgccccggct ttccagttca ccagattgcc
    33661 cgacttggcg gtgtcgacgg tggtgaccgg agaacctttt gccacaccag ctgggcaggt
    33721 gatggcaccg cgtccgctgc cgccgcaggc ggtggcactg tcatattcag tatgataatt
    33781 atccagacgg atcccgccgt tcagctcaaa atcacgggtg atttgcagcg tatcgaaggc
    33841 gtaaattgcg aaggtatccg tctgaccatt ggcgtttgcg ccgttgcgcg tcaggccgcc
    33901 gggatgaatg ctgctgtcag gatgataaat atttaccgcg ggtaacgtca ccggattaac
    33961 gccgtagtta gtctgcgttt cacgggtaaa ttccacgccg gtactgacat catgaccgat
    34021 agaaccggta tagaacgtcg aggtcaggtt ggtctggttg gtgagaattt tattactcac
    34081 atctttggta ttcgccgtgc gtgaccaggt ccagctattc acatcgctgg tgggctgagt
    34141 aatattcgac gccccgccca taatcgccgt catcaggtaa tcctgcttta cgcgcgacca
    34201 acgggtagta ttgcgaatgg tggtgttatc gttgatgtcg tgctcaaaac gcatggtggc
    34261 ggtgtcggtg gtcgaatcgt cgtaatcgga atccgtgccg taaaagttat gagtatcaac
    34321 ttttccggaa tgattcagag ccgctgttcc cgcagatggg gcagaataac ccggcaaacc
    34381 gatggtcgga atgccgccgt ctggcgtgtt gtgctgggtg acatgcagat aattaagata
    34441 caaacgattc gctgtaccaa ggccaaaagc gatagaaggg gcgacgccgt aacgctcatt
    34501 tttgactttg tcgcgtccgg catcgtgcgt tttttcgccc atcacattca ggcgcaccgc
    34561 agtggtatca ccaatgacct gattgacgtc cagcgtgccg cggcggaacc aggcgctgcc
    34621 aatactggcg gaggcgtcaa tgccggaatc attgcgcggc tgcttgctga tcatattgat
    34681 cgagcccgtc ggcgcgctgc gcccgtagtc ggtgccggaa ggccctttaa tcacttcgac
    34741 ctgctcagta ttgaaggtgt cgcgcgagac gctgccgata tcgcgaatgc catcaatata
    34801 aatactgtta gaggtatcag caccgcgcat ataaatggcg tcgccagtgg tggagttacc
    34861 gttctcaccc gcaaaaaacg cacccacgcc ggggacgttt ttgagcgcgt cggtaaggtt
    34921 ggttgcgccc tgatctttaa tcacttgttc agaaatcacc gtcatcgtgc gggtagtatc
    34981 cgctaccgga cgcgagaatt tcggatcggc agattgttgt ggcgcataaa gcgaaggcgt
    35041 tgatgcttcg acaaccagcg tgtcatccgc gttagtttgc ccttcggcgg cgagtgcctg
    35101 agccacaggc gtgatgccaa tacaaagacc ggcaaagaac gtgagcgaat gaaattgtct
    35161 ggcagggaaa ttgcgattgt tttccatttt gcaggtgact ttttttatat gttgaaattt
    35221 tcactgccac gagaaagcct ctctgcggat gcgatgccac tgttaatctc gtgatagtgt
    35281 tcgtgcaagt gataataatt ttgataagca ttatcacttg gcgcgatttt ctatcaccca
    35341 ttgcgcgaaa aagaaataca tttatttaca ttgcgttcat atttgatgtt ttttgattac
    35401 aaaacaggcg aaaactgcat ccagaggcga atgcagttac gttggtgggg ttatttgtag
    35461 atggttgcag taccgaacat ctggtcattt ccgcctgcgg aattaatgac ataacctttc
    35521 gcgccttgct cgcgcgcttt ttctgccagt ttatcctcca gatcgcttag gtttgatgcg
    35581 ccagttgccg aaacggtacc ggcgggccgt agctgaccgc tgtccaggtt actcatcggt
    35641 tgcgcggcat aagccgtgag agagattccg ctcaggacgg tggcaatcag tagtgtgagg
    35701 cactttttca taatgacatc ctcataaata caacgggtag tgcctgataa gtgtaggtcg
    35761 cctgggcgat gaaaatgacg caaaaaattg acagagatgt gcttattttt tgaacgatat
    35821 atttttacaa aataagacaa atcaatgaca tgcataatgc tgatttttat gatgaattgc
    35881 cataaaccat agccatgtta aggttttggg gaatgataaa cgcagccaga tatttaccct
    35941 gtcgcgttat gcgggtatct tacgccgctg cttaaaggag aatgctatgt ccgcccagaa
    36001 accggggttg catccgcgca accgtcatca cagccgctac gatctcgcca cgctttgtca
    36061 ggtcaatcct gaactcaggc aattcctcac gcttacaccc gcaggggagc aaagcgtaga
    36121 ctttgccaat ccgctggcgg tgaaggcgct caataaggca ttgctggccc atttttacgc
    36181 cgtagcgaac tgggatatcc cggacggttt tctctgcccg ccagtaccgg gccgggcgga
    36241 ttatattcat caccttgccg atttactggc agaagcaagc ggaacaattc cagctaatgc
    36301 cagcattctg gatatcggcg ttggtgcgaa ctgtatttat ccgctgattg gcgtacatga
    36361 atatggctgg cgttttaccg gtagtgaaac cagcagtcag gcgttaagta gtgcgcaggc
    36421 gatcatcagt gctaatccgg ggcttaaccg cgccattcgt ctgcgtcggc aaaaagagag
    36481 tggggcgatt tttaacggca tcatccataa aaacgagcaa tacgacgcga ccttgtgtaa
    36541 cccgccattc cacgattccg ccgctgcggc acgggcaggt agtgagcgta aacgccgtaa
    36601 cctggggctg aacaaagacg atgcactgaa ctttggcggc cagcaacagg agttgtggtg
    36661 tgaaggtggt gaagtcgcct ttatcaaaaa gatgattgaa gagagcaaag gcttcgcgaa
    36721 gcaggtgatg tggtttacct cgctggtttc tcgtggtgaa aacttaccgc cgttgtatcg
    36781 tgctctgacg gacgtgggcg cggtgaaggt ggttaaaaaa gagatggccc aggggcaaaa
    36841 gcagagtcgc tttattgcct ggacctttat gaacgacgag cagcgccgcc gttttgttaa
    36901 tcgccaacgt taaagcgttg gctcccccgg cggcagcggt tcagccgggg ccgcgcccgg
    36961 agcaggcagc acctgataag tctgcactgg cgcgcgaaca cctgccaggt cgaaatgctt
    37021 tttcacctgg ctgtcgaggg caaagcggac cgtccactgt ttgagtggca gcgtggtgaa
    37081 cgaaacacgc agtgtaaacg cggtattgct taagccgaca atcccggcaa agtttggttc
    37141 accaataacc agcccgcgaa tttcttcgtt ttccatcaat tccgctaccg catctttcag
    37201 tgcctgattg gctttatcag catcttcatg gcgatcaaca tcgtagtttg ccaccaccga
    37261 accaatgccg cggacgaagt tagcaaaggt ggttatcgaa gaccaaggaa tgatgtgata
    37321 cgccccggta tcctgacgca cgcccacgga gcgaatcgac atccgttcca cagtgccggt
    37381 caacggcccg atagtaacca aatctccagt gttcatgccg ttttcaaact gaataaatac
    37441 cccggtgata atatctttca ccagcgtttg cgaaccaaac gagatagcca gacctaatgc
    37501 cccagcacct gccagcaatg gcgcgatatt gacgccgatt tccgacaaca caatcatgat
    37561 ggtgatggta ctgataatca ccgccagcgc gttacggaac agcgtcagca gggtacgcgt
    37621 acgggcgctg ggtagcgggc ggccatgaat atccgaagcc agccggtttt cgatcaaact
    37681 ggcgagcact gtccagccaa ccgccgagaa gaaaagaatg agtgcgatgc ggatcaggat
    37741 atctacggtt ttctgccccg cgccgttttg cagccagttc cagaaatcga acaatcccca
    37801 tgcgctcaac agcaacatta ccgccacgca gacggtcaga atacgcgccg ttttcagcgc
    37861 cgccgaaagc cagccattca gccgtttttg tagctccgga tagttacgct gagtatgtgg
    37921 cgagagggtg atggttttcg ccagccagcg ggaaaacata ccggaaacaa acgctgcgat
    37981 accaataatc gccaggctgc gcaccgttgc acccatcata aatttcaggc tgttgcccgg
    38041 atcgaacaac gaaaagaaaa acagcacgat aaaataggcg cttgccagcc agtgccacac
    38101 cagcgcaaag gcgcggataa acaggctgaa aaaggccagc gaatgctccg caaagttgag
    38161 caaatgttgg gtaatctctt ttttattgcg aaagatcagg tacaacgccc agacggtcat
    38221 gcacagcata atgatgacgt tcgccagcgc acctatctgt acattcacct gattagagat
    38281 aatcggcacg gccacaatca ggccataacc tatcaaactg cttaaccagc tcaggcgacg
    38341 actccagtaa cgggcgctct cgtcctgaat cgtgaacggg cgcagttcag ccacgtttgg
    38401 gcaaaaaatc aggcgtagta cggctttgaa aaattcaatc agggcaaagg cgttgagaaa
    38461 caaactttgt tggaaagcga tggtgcgact gcccgcattc aggttgtcgc ttaatacctg
    38521 gccgacaaac aatgtcagtg ccagtaacag taggtcgata ataaacgccc cgataatcat
    38581 cgccggaagc tgcagccagt tgctgcgttc gcgatttttt tgccgcgccc actggcccat
    38641 tttgcgatac agcggcagtg cgcacagacg aatcagccag taaaaaccaa acactaatac
    38701 cgctaacatt gaaaaatggg tcaacgcatt gctgaaggtt tgtggattaa acggcttatg
    38761 cggggagccg gtgatattgc gataaagttg tccgaagcgg gcggaaaggg cttcaccata
    38821 atggcggctg acttcggtga ccttttgcag cacggtttgc tcttcaacca gcgtcggcgg
    38881 cacgatcttc gggaccggtt ccgcaggggg cgtcgcggcg acagtacgca actggtcgat
    38941 caattcttta cgcgaggtgt cgttatccag cacatccgcc agtgcgccat aggccgcttt
    39001 tttttgttcg atatccggtt ccggggccgg ttcagtcgtt gagtccgttg tcgttgtggt
    39061 tgtaacgccg ggtatggata ccgcgtgggc aggtgcgccc agcaggcaga agaggatgaa
    39121 caggatccac cgcatgactc ctccagtgag aaaacagagc aaaaagataa gtatagatgc
    39181 tgggggaggg gcgattttaa atgagaggaa tctggtgtgc ctccctttcg ggtgaaaggg
    39241 aggcagagaa gttaagagac gtgctgcaaa aattcctgca agcgctggct cggcgggttc
    39301 ttgatcaata cctgcggatc gccatcttcc gcaatccggc ctttgtcgat aaagatcagc
    39361 cgcgaagcta ctttctcggc aaaaccgatt tcgtgggtca cgatcaccat cgtcatccct
    39421 tcttcagcca gatcctgcat aaccttcagc acttcatggc gcagttccgg gtcaagagcg
    39481 gaagtcggtt catcaaacag catcattttc ggcttcaccg ccagcgcgcg ggcaatcgcc
    39541 acacgctgct gttgaccacc agaaagttcg gaagggtagt gatgtgcacg ttctgccaga
    39601 ccgactttcg ccagcagctc acgcgccagt ttttccgcct cttctttgtt cgcgccacgc
    39661 acgcgtagcg gaccaaacat gacgttttcc agcgccgtca gatgcgggaa gaggtaaaac
    39721 tgctggaaca ccatacctgc ttcctggcga atcaggcgct cgtcaacttt cggatcgtta
    39781 accttcaggc catcgacaat cagatcgccg gaggtgattt cttccagttt gttgatgcag
    39841 cgcagcaggg tcgatttacc ggaaccggac ggcccgataa tcaccacgac ttcgccctgg
    39901 gcaatgttca aatcgatatt gtgcagcacc tgggttgggc caaagtgctt ggagacgttt
    39961 ttaaattcaa tcacaggatt ttcatccttc tttccagacg acgcagaata aagctcagca
    40021 ccagggtaat aatcagatag aacaccgcca cggcgctcca gatctcaagg gcgcggaagt
    40081 taccggcaat aatttcttgc ccctgacggg tcagttccgc cacgccgatc acaataaaca
    40141 gcgaggtgtc tttaatgctg atgatccact ggttacccag cggcggcagc atacgacgca
    40201 gtgccagcgg taaaatgacg tagcgaatgg tttcccaacg tgaaagaccg agcgccagtc
    40261 ctgcttcacg aaaacctttg tggatagaca gcaccgcacc acgcgtgatt tccgcaatgt
    40321 acgcgccgga gttgatcatg atggtgacca ccgccgcagt aaatgggtcg atgcgtaagt
    40381 cgttaaacgc catcggcagg gcgaaataga taaacatcac ctggacgacg ataggtgtgc
    40441 cgcggatcac ttcaataaag accagcgcga cgtggttggc tatccaacca ccgaaggtgc
    40501 gtgcaaaacc tgccagcaat ccgattacca gaccgcctgc cagaccgagg accgaaatcc
    40561 acagggtcat tttggcacct tcaatcagaa gcggaatggc aggccagatg gcactccagt
    40621 caaactgcat atgttgttcc tgttaccgtg gtgtaaataa tcaaattcag ggtaattaat
    40681 ggccccggag cgggtttgcg cccctcaccc taatcctctc cccatagggg agagggaact
    40741 gccagtgcgt tttacaggtg tagcgttatt atttcggttc agtaccgaac cattttttgt
    40801 agatttcgtt gtaagttccg ttctcgcgca gggttttcaa cgcgccgttg actttgtcac
    40861 gcagctcgtc gctacctttc gggaacgcaa taccgtattg ctgcgcttcc agagagtcac
    40921 ctaccgcttt gaactgaccg ttaccggcgg ttttgatgaa gtacagaatg tttggcgtat
    40981 cgtgcagaac ggcgtctgcg cggttggtgc ccagttccat ataggcgtta tcgatgttcg
    41041 ggaactgacg cagatcttta gttttgatgt ttgctttcgc gtaatcaacg gagccagtac
    41101 cgctcttcac agcaaccact ttcccgtcga gatctttcac gcttttcaca tcgttattgt
    41161 tagctttcac catcactaac aggccgcttt tgtagtagcc gtcagagaaa tcgatcgctt
    41221 ttttacgctc gtcggtgatg gtaatgcccg ccagcgccag atcgacgttt ttggtttgca
    41281 gtgccggaat gatcccactg aaatccatcg gcttcagttc gtaatccagc ttcagctctt
    41341 tagcgatggc agcccacaga tcaacgtcaa agcccacata tttatcgccc tgtttaaatt
    41401 caaacggaac gaaggcggta tccgtcgcga caactaattt tttatccgcg gcatgagaag
    41461 aaaccgcaaa agccagggtc agtgcagcca gtgaaacttt taatacagac ttcatagcat
    41521 ttcctttttt ctatccacgg gacgatcccc tgcgtgacat tcacatatat gaaaaaatcg
    41581 tgccagtttt gcaactcctt gatttttcga gatgcggcaa aaacgtgatg cacgatttat
    41641 agggcaatac cctgaagatg caccattctg gggcaccaat ctggtgcgct aaaattgtgc
    41701 actcaacaca gtgattattt agcgtaaaac ctgatgaaaa aacaatcttt atgtaacgat
    41761 tgtgtgatga tgtggataca aaaaatttac ttaatcagct ggagatagca gatggatgca
    41821 ctaaataagt gcgttgaggt gggctgcaaa acaaaacggc ctcctgtcag gaagccgctt
    41881 ttatcgggta ctaaagttct gcaccatcag cgatggattt attcgatgtt agactcgata
    41941 aaccacagga atttatccag gtcgcgagac gcggcggtca ggatatctgc ggtgtcgtca
    42001 tctttcgctt cgccaatcgc tttgcgtacg tcattagcga cgattgcgta acggtcagcc
    42061 agttctttca ggtgatcctg aacgttgtgg atgtccagcg ggtaactttt cagcggggtt
    42121 ttgctgttga taacttgagt ggtccccaga gctacaccgc ccagctgcac tgcacgttct
    42181 gccatggtat ccagatgatc gatcagtgcg gtgcggaagc catccagcat ttcatgtacg
    42241 gcaatgaagt tagcgccgcg catgttccag tgcgcttgtt tggtaatcaa agaaagatca
    42301 ataaactgga taacctggcg attcagcaac tctactgttg cttttttctc gctgtcggag
    42361 acatcgttgc gggtataaag cagattggtc gcttttgatt taactaattt agcggtactc
    42421 ataatttcat atcctcttga tgttatgtcc cagtaattaa cgagactaag tatagcaccg
    42481 gctatgtgtt ccgctattct ggctgttcct atcacactaa tagtggtaac aagcgtgaaa
    42541 aacaaaacta ataaagattc aatgagttag atatattgat aagaacaatt cttatttatc
    42601 tgctcgccag gaattttcgg aataaatatg cttttgcata attcgatata taaagacggt
    42661 gtagaggaaa agtagcgaga aattctgcat ggttatgcat aaccatgcag aaatgtaaat
    42721 ttaattaatg tctaattctt ttattttgct ctctttgcgt actgtcagcg tagaccccat
    42781 tgaagcggcg atgatagcgc cgagcgccag cagctgtatg ggtgtcagtg tttctccgag
    42841 gaaaatcatc ccggaaacgg cagccagcgc cggttccatg ctcatcagcg taccaaatgt
    42901 ccgtgttggc aaacgggtga gggcaatcat ttccagcgaa taaggcagag cggtcgagag
    42961 aatagcgaca gccagaccca atggaataac cgaccagtgc cagagtgctt caccagcctg
    43021 aagcgctcca attggcacga aaattaacgc tgcaatcaac gaaccaattg ccaccgtcgc
    43081 agggccatgt tccgctcctg cgcgttgccc acttaaaatg taaatagccc aacaagcccc
    43141 ggcccccagt gccagcgcac agccggttaa atcgacatgg gaaacgtctt gccccagcgg
    43201 tagcaggaac cacagaccaa gaaccgccag cacaacccag acgaaatcta ccgggcgacg
    43261 agaagagaac agcgccaccg ccagtggtcc ggtgaactcc agcgccaccg caatacccag
    43321 cggtactgtc tgaatagaaa gataaaaaag ataattcatc ccacccagcg aaacgccgta
    43381 aaacaacagc ggtaaccgtt gctctttggc aaagcgcagt cgccatggct taaagaacgc
    43441 gatgaggatc agcgttccta atgccagacg cagcgcagtg acacccggtg cgcccaccag
    43501 aggaaaaagt gacttagcta acgaggctcc accctgaata gacgccatgg caacgagcaa
    43561 tatgattatt ggtaaccaga ccggcatttt acgtaatgaa ccaggcatcc tttctcccac
    43621 aaatatctag acttaagtaa agcgtggagt gtactggata tacccaatgc tggttgagca
    43681 tttgttgaaa aaattttccc ccgttttgac taaaatgcgc caggattgat ggaatcatta
    43741 gtctggtgat taggaataat ctggatgaat gacagggaaa acatgcgtaa tacttacgca
    43801 gttctctgaa aaagtgattt aaatttagat ggatagcggt gtatggaaac gttctgttac
    43861 atgaaatggc ccgttagaca tcacaaatcg cgaagagttt cccattaatt tttgatatat
    43921 ttaaaactta ggacttattt gaatcacatt tgaggtggtt atgaaaaaaa ttgcatgtct
    43981 ttcagcactg gccgcagttc tggctttcac cgcaggtact tccgtagctg cgacttctac
    44041 cgtaactggc ggttacgcac agagcgacgc tcagggccaa atgaacaaaa tgggcggttt
    44101 caacctgaaa taccgctatg aagaagacaa cagcccgctg ggtgtgatcg gttctttcac
    44161 ttacaccgag aaaagccgta ctgcaagctc tggtgactac aacaaaaacc agtactacgg
    44221 catcactgct ggtccggctt accgcattaa cgactgggca agcatctacg gtgtagtggg
    44281 tgtgggttat ggtaaattcc agaccactga atacccgacc tacaaacacg acaccagcga
    44341 ctacggtttc tcctacggtg ctggtctgca gttcaacccg atggaaaacg ttgctctgga
    44401 cttctcttac gagcagagcc gtattcgtag cgttgacgta ggcacctgga ttgccggtgt
    44461 tggttaccgc ttctaatcac tttggtgata taaaaaatcc gcctctcggg gcggattttt
    44521 gtttttaagg cttcgggtcg aaaatatcgg ttccgagatg gttgtaatcc accttctgta
    44581 actggaagtt ggtgatgtat atcgacccgg ctttcttctc agatataaac gggtatttga
    44641 tgttaatttc cttcgcttta attcccgtcc actgggagaa aaaacctaag aagtcattgg
    44701 ctgagcggcg ggctttaatc acccgatgcg ctttatcgtc gctggaaatg accataaaag
    44761 gcacctggaa attttgctga tacttatcat catgggcaag gtattgcacg tctttgccgc
    44821 gctctttaaa ggccagaccg tggtcagaaa agtaaaccag cgagaagctg ctgccgctgt
    44881 tgcgtaactg atcgtacagc ttgcgcagta aatcgtccgt ttgcgtcatg gtatagagat
    44941 agcacgacgt ttctttcgac tgcacaaagg tttcgtattt gccttgtgtc ctgtcgcagg
    45001 cctgcggatg tgagcccatc agatgtagaa caatcagctg cggttgcgag tgctcttgcg
    45061 ccagcacttg agcggtcata tccagtaacg cttcgtcttt ggtattttta tctgcttcaa
    45121 aattaccttc tttcaggaag tacacttcat ctgctcgttt ggcgatgctg gcgatagcgg
    45181 tatcgtattc gccgatttga ccctggttgg aaaaccacca ggtctggaag cccgcgcgat
    45241 ttgccagggt gacaaagtta tcctgaaact gtggtttgcc atcgactacg cgattgagcg
    45301 ttaagccaag cgatttctgc gtggagccac tggcggcaat gtagtcagca aatatcaaac
    45361 cgttaacgct gctggcaaac ggggtattgt cccagtgacc gccaaaggca ccgagggcat
    45421 cgcgacgcgc gctttcaccg atcaccacca cataggtctg atacttcggc ttaacggcag
    45481 tgaccgtcca ggtatctttc attccggaaa gttttgccat ccgttcttgc tcttcaatca
    45541 cctcgttatt attgacgatg acatccttag caaaacgaaa taccggatag ccagtatctt
    45601 tcagtttaaa tacgccgccc caggccaggt tttgcacggg agcaacaaaa aatgtcgcca
    45661 cactgaatac cagacacagg ctgtcgaagg tattccagcg cgctttttca ctctcttttt
    45721 ttcgcctgat tgctattacg ccgagcacaa aaataaatat gccgaccatg tagctgtacc
    45781 acggaaaaat cgtcaggatt tcggtcgact cttccatatt ggtggagtgc aatgccagca
    45841 atgtattgaa attaggcgcg ccgtaggcct gagcaaaagg gaaataacaa gccgccacca
    45901 gcgaactgac accgaccaga actttttgta cgcgaggcaa tgttcgccat agcaaaagca
    45961 aaatagccgt aaacgcagcg gtgtagagca aactgaaggg gtaacctaag ccgaggttaa
    46021 ttaatagcga ctgtaaaaag tagaacgcag tccacgggct aaatacccgg ctacgggtaa
    46081 caagcgattc tttgagggtt aaattcatat gccactatcg taacaaacgc catgtgctta
    46141 ccctggcgtc aagggtcaat acctgcaaga gtgcgtgaag agtggaaagg ggtccgcatc
    46201 cgcgagccgc aatatacgca gggctgcaag aagatagagc gagccgccgt tcaggtcaac
    46261 tactgacgaa caaatctttt gcgaagggga ttgcaaatcc gacaaaaagt ggggtttttc
    46321 agaccaaaac gcgttatgcc tgcatgaaag caggagtgaa gcggcgtacc gcgacgccgc
    46381 tatttatcgg aaggtttatc ttgctgcggt ttgttgttga ccatatcgca caacatagag
    46441 agcagcatta accgtacttt aaagggagaa tgactaaaca cgcgcataca cctcttgaac
    46501 tcattcataa gacctcctga cttgctaatc ccgtcgatcc ttgagggatg attgcattac
    46561 atacagatat agcacaggct atattatata gctattgcta aaacgttaat tttttgtacc
    46621 ttcgcaactc tggtttacaa tgtgcgcacg aaatgagaac gctatgtatg cgtcaccata
    46681 atgaggaagc acaatgagtc gtcgcgcagg tacgccaaca gcaaaaaaag tgacgcagtt
    46741 agtgaacgtg gaagagcacg ttgaagggtt ccgccaggtc agagaggcgc atcggcgcga
    46801 gcttattgat gattacgttg agctgatttc tgacttgatc agggaagtgg gggaagctcg
    46861 tcaggtggac atggctgctc gtctgggagt ttcgcaaccg acggtggcta aaatgcttaa
    46921 gcggctggca accatggggc tgattgaaat gatcccctgg cgtggcgtgt ttttaacggc
    46981 agaaggagag aagctggcgc aggaaagccg cgagcgacat cagatagtcg aaaatttctt
    47041 gttggtgttg ggcgtcagtc cggaaatcgc ccgtcgcgac gcggaaggca tggagcacca
    47101 tgttagtgaa gagacgctcg acgcttttcg tttgtttacc cagaaacacg gtgccaaatg
    47161 agcctgcctt ttttacgcac gctgcaaggc gatcgttttt ttcagttatt aattcttgtt
    47221 ggtatcggat taagcttttt cgtgcccttt gcaccgaaat cctggcctgc tgctatcgac
    47281 tggcacacca tcatcacctt aagcggcctg atgctgctga ccaaaggtgt ggagttaagc
    47341 ggttattttg atgtgctggg gcgcaaaatg gtgcgccgct ttgctacgga gcgtcggctg
    47401 gcgatgttta tggtgctggc ggcggcgctg ctttctacct ttctgaccaa cgatgtcgcg
    47461 ctgtttattg ttgttccgct gactatcacg ctaaaaagac tgtgtgagat cccggttaat
    47521 cggctgatta tttttgaggc gctggcagtc aacgctggtt cgctactgac gccaattggc
    47581 aacccgcaaa atattcttat ctggggacgt tctggtcttt cgtttgccgg atttattgcc
    47641 caaatggcac cgctggctgg cgcaatgatg ctgacgctcc tgctcctgtg ctggtgttgt
    47701 ttccctggaa aggcgctgca ataccatacg ggggtgcaaa caccggagtg gaaaccgcgg
    47761 ctggtgtgga gttgtctggg gctgtatatc gtctttctga cggcgctgga gttcaaacaa
    47821 gagctgtggg gactggtgat tgtggcggca ggctttgcgc tgctggcacg tcgcgtggtg
    47881 ctcagtgtgg actggacgct gctgctggtg tttatggcga tgtttatcga cgtccattta
    47941 ctgacccagc ttccagcgtt gcaaggcgtg ttgggtaacg tgagtcatct atctgaaccc
    48001 gggttatggt taacggcaat cggtttatcg caggtgatca gtaacgtgcc gagtaccata
    48061 ttgttgctga actatgtgcc gccgtcttta ttactggtat gggcggtaaa cgtaggtggc
    48121 tttgggttat tacccggatc gctggcaaat ttgattgcgc tacgtatggc gaacgatcgc
    48181 cgcatctggt ggcgtttcca tctctattca ataccgatgc tgttgtgtgc ggcgttggtg
    48241 ggatatgttt tgttagttat actcccggcc aactagggtc tggcaaataa aaaaggcgga
    48301 ttatgagtcc gccttttgct ttaagttttg aagattaatt cagacgaacc ggcatcccgg
    48361 agcggttttt aatcgcttca tcaagaacaa cctggtcaac atctggctga ccggtcactg
    48421 tttgcacgct cttcgtcagg gtaattggca caatttcctg accttcaaac tgggcttcgg
    48481 tggtagacag cgggttatgg acttcaatat aacggctgcc gtctggctcg gtggtcgctt
    48541 ttaccggctc atcaataaac tgtacgcggg taccgaccgg tactttctcg aacaggaatt
    48601 tgatgtcttc gttacgcaga cgcacacaac catgacttac acgcaggccg ataccgaagt
    48661 tggcgttggt gccatggata gcgtacaggc gaccgatgta gagtgcatac agccccatcg
    48721 ggttatccgg acctgccgga acgacagccg gaagcggttc gcccgcagcg cggtactctg
    48781 cgtgcatttt ggcggtcggc gtccaggtcg ggcctgcttt cttacgctca actttagtgg
    48841 tccagttgat aggcgtatct ttgcctaact gaccaatgcc gatcggcagc acgataacgg
    48901 tgttggtccc tttcggatag taatacagac gcatctctgc actgttaatg acgatgcctt
    48961 catgaacggt atccggcagg atcagctgct gcggaatgtt cagtacagta ccgcctttcg
    49021 gcaggaaggt atccacaccc gggttcgctt ccatcatatt ggaaagcccc atctggtact
    49081 ccgcggcaaa atactccagc ggctgagtgt taccttcagg aatggtgatc acctgattct
    49141 gaccaaccag gcgactcccg tcggttggca gaggataagt taccgcagag gcggtactgc
    49201 aaaagccgac aacagcgaag gccgctgcga ataatgtttt caatttcata ttcatgttaa
    49261 gcgagatttt gtgcctggca ggccattggg ttgagaatat tagagtattg gaagcgcatt
    49321 ataagttcat tccagctcac agtgaaatca gatgtgtacg aaatcacatt ttttgccttt
    49381 ggcttgagtg tagaccttaa gcgaggagca ggatcttctt tcagacttat ggcataatgc
    49441 gcggtttgtc atatctcttt tcaggatacg cctgtgttag tttccagtaa cgtcaccatg
    49501 cagttcggca gtaagccgtt gtttgaaaac atttccgtca aatttggcgg cggcaaccgt
    49561 tacggcctga ttggcgcgaa cggtagtggt aaatccacct ttatgaagat cctcggcggc
    49621 gacttagagc cgactttggg taacgtttcc ctcgatccca acgagcgcat tggtaagctg
    49681 cgtcaggatc agtttgcctt tgaagagttc actgtgctgg atacggtgat catggggcat
    49741 aaagagttgt gggaagtgaa gcaggagcgt gaccgcatct atgctttgcc ggaaatgagt
    49801 gaggaagacg gctataaagt ggccgatctg gaagttaaat acggcgaaat ggacggttac
    49861 tctgcggaag ctcgcgccgg tgaattgttg cttggcgtgg gaattccagt ggaacagcac
    49921 tacggcccga tgagtgaagt tgctcctggc tggaagctgc gtgtgcttct ggcgcaggcg
    49981 ctgtttgctg atccggatat tctcctgctc gacgaaccga ccaataacct cgacatcgac
    50041 accattcgct ggctggaaca ggtgctgaac gagcgtgaca gcaccatgat catcatctcg
    50101 cacgaccgtc acttccttaa catggtttgt acccacatgg cggatctgga ttacggcgag
    50161 ctgcgcgttt atccgggcaa ctacgatgag tacatgacgg cggcgaccca ggcgcgtgaa
    50221 cgtctgctgg ctgataacgc caagaagaaa gcgcagattg ctgagctgca atctttcgtt
    50281 agccgcttta gcgccaacgc ctcgaaatct cgccaggcaa cttcgcgcgc gcgccagatt
    50341 gataaaatca aactggaaga ggtgaaagcc tccagccgtc agaacccgtt catccgtttt
    50401 gaacaggata agaaactgtt ccgtaacgcg ctggaagtgg aaggtctgac caaagggttt
    50461 gataacggtc cactgtttaa aaatctcaac ttgctgctgg aggtcggtga aaaacttgcg
    50521 gtactgggta ccaacggcgt cggtaaatca acgctgctga aaacgctggt gggcgatctg
    50581 caaccggaca gcggcaccgt aaaatggtct gaaaacgcgc gcattgggta ctatgctcag
    50641 gaccacgaat atgagtttga aaatgatctg accgtgttcg aatggatgag ccagtggaag
    50701 caggaaggcg atgacgagca ggcggtacgc agcattctcg gtcgtttgct gttcagccag
    50761 gacgacatca aaaagccagc taaagtgctt tccggtgggg aaaaagggcg gatgctgttt
    50821 ggtaagttaa tgatgcagaa gccgaacatt ctgatcatgg acgaaccgac caaccacctg
    50881 gatatggaat ccatcgagtc actgaacatg gcgctggaac tgtatcaggg cacgctgatc
    50941 tttgtttcac acgaccgtga gttcgtaagc tccctggcga cccgcattct ggaaatcacc
    51001 ccggaacgcg tgatcgactt tagcggtaat tacgaagatt acctgcgtag taaagggatc
    51061 gagtaagtga ttatccgtca ccccgatact gtcggggtga cggcggctga tgacaaatgc
    51121 aaaat
//
LOCUS       DAZFXT010000018        49906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.018, whole genome shotgun sequence.
ACCESSION   DAZFXT010000018 DAZFXT010000000
VERSION     DAZFXT010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.018"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1749
                     /gene="adeD"
                     /locus_tag="ACHKW0_001192"
     CDS             <1..1749
                     /gene="adeD"
                     /locus_tag="ACHKW0_001192"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HFO7474060.1"
                     /translation="HKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISG
                     PIVIKGRYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATL
                     PRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASF
                     TLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELN
                     AYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLC
                     TDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQ
                     ADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDF
                     ALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGL
                     LGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHL
                     PLPIAGLMSTDTAQLLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQG
                     LFDGEKFAFTTLEVTE"
     gene            complement(1794..3185)
                     /gene="uhpT"
                     /locus_tag="ACHKW0_001193"
     CDS             complement(1794..3185)
                     /gene="uhpT"
                     /locus_tag="ACHKW0_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HFO7474061.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3323..4642)
                     /gene="uhpC"
                     /locus_tag="ACHKW0_001194"
     CDS             complement(3323..4642)
                     /gene="uhpC"
                     /locus_tag="ACHKW0_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HFO7474062.1"
                     /translation="MLPFLKAPADAPLMTDKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            complement(4652..6154)
                     /gene="uhpB"
                     /locus_tag="ACHKW0_001195"
     CDS             complement(4652..6154)
                     /gene="uhpB"
                     /locus_tag="ACHKW0_001195"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HFO7474063.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6154..6744)
                     /gene="uhpA"
                     /locus_tag="ACHKW0_001196"
     CDS             complement(6154..6744)
                     /gene="uhpA"
                     /locus_tag="ACHKW0_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HFO7474064.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(6819..7109)
                     /gene="ilvN"
                     /locus_tag="ACHKW0_001197"
     CDS             complement(6819..7109)
                     /gene="ilvN"
                     /locus_tag="ACHKW0_001197"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HFO7474065.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7113..8801)
                     /gene="ilvB"
                     /locus_tag="ACHKW0_001198"
     CDS             complement(7113..8801)
                     /gene="ilvB"
                     /locus_tag="ACHKW0_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HFO7474066.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(8907..9005)
                     /gene="ivbL"
                     /locus_tag="ACHKW0_001199"
     CDS             complement(8907..9005)
                     /gene="ivbL"
                     /locus_tag="ACHKW0_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HFO7474067.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            9570..9659
                     /gene="tisB"
                     /locus_tag="ACHKW0_001200"
     CDS             9570..9659
                     /gene="tisB"
                     /locus_tag="ACHKW0_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HFO7474068.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            complement(9922..9996)
                     /gene="ysdE"
                     /locus_tag="ACHKW0_001201"
     CDS             complement(9922..9996)
                     /gene="ysdE"
                     /locus_tag="ACHKW0_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsdE"
                     /protein_id="HFO7474069.1"
                     /translation="MNVSQIYARNGELFSGRICKQKRQ"
     gene            10078..11262
                     /gene="emrD"
                     /locus_tag="ACHKW0_001202"
     CDS             10078..11262
                     /gene="emrD"
                     /locus_tag="ACHKW0_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HFO7474070.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11270..11767)
                     /gene="yidF"
                     /locus_tag="ACHKW0_001203"
     CDS             complement(11270..11767)
                     /gene="yidF"
                     /locus_tag="ACHKW0_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HFO7474071.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11764..12126)
                     /gene="yidG"
                     /locus_tag="ACHKW0_001204"
     CDS             complement(11764..12126)
                     /gene="yidG"
                     /locus_tag="ACHKW0_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HFO7474072.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12116..12463)
                     /gene="yidH"
                     /locus_tag="ACHKW0_001205"
     CDS             complement(12116..12463)
                     /gene="yidH"
                     /locus_tag="ACHKW0_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HFO7474073.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12572..13021
                     /gene="yidI"
                     /locus_tag="ACHKW0_001206"
     CDS             12572..13021
                     /gene="yidI"
                     /locus_tag="ACHKW0_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidI"
                     /protein_id="HFO7474074.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13068..14561)
                     /gene="yidJ"
                     /locus_tag="ACHKW0_001207"
     CDS             complement(13068..14561)
                     /gene="yidJ"
                     /locus_tag="ACHKW0_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7474075.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(14558..16273)
                     /gene="yidK"
                     /locus_tag="ACHKW0_001208"
     CDS             complement(14558..16273)
                     /gene="yidK"
                     /locus_tag="ACHKW0_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HFO7474076.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKIDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRSTPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLSMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            16440..17333
                     /gene="yidL"
                     /locus_tag="ACHKW0_001209"
     CDS             16440..17333
                     /gene="yidL"
                     /locus_tag="ACHKW0_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7474077.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(17330..18144)
                     /locus_tag="ACHKW0_001210"
                     /pseudo
     CDS             complement(17330..18144)
                     /locus_tag="ACHKW0_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18144..19760)
                     /locus_tag="ACHKW0_001211"
     CDS             complement(18144..19760)
                     /locus_tag="ACHKW0_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7474078.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGQFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            20056..20772
                     /gene="yidP"
                     /locus_tag="ACHKW0_001212"
     CDS             20056..20772
                     /gene="yidP"
                     /locus_tag="ACHKW0_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7474079.1"
                     /translation="MIYKSIAERLRIRLNYADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IGQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(20769..22430)
                     /gene="yidE"
                     /locus_tag="ACHKW0_001213"
     CDS             complement(20769..22430)
                     /gene="yidE"
                     /locus_tag="ACHKW0_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HFO7474080.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(22627..23055)
                     /gene="ibpB"
                     /locus_tag="ACHKW0_001214"
     CDS             complement(22627..23055)
                     /gene="ibpB"
                     /locus_tag="ACHKW0_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpB"
                     /protein_id="HFO7474081.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(23167..23580)
                     /gene="ibpA"
                     /locus_tag="ACHKW0_001215"
     CDS             complement(23167..23580)
                     /gene="ibpA"
                     /locus_tag="ACHKW0_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small heat shock chaperone IbpA"
                     /protein_id="HFO7474082.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(23574..23646)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            23886..24218
                     /gene="yidQ"
                     /locus_tag="ACHKW0_001216"
     CDS             23886..24218
                     /gene="yidQ"
                     /locus_tag="ACHKW0_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7474083.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(24220..25434)
                     /gene="yidR"
                     /locus_tag="ACHKW0_001217"
     CDS             complement(24220..25434)
                     /gene="yidR"
                     /locus_tag="ACHKW0_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HFO7474084.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            25535..26599
                     /gene="cbrA"
                     /locus_tag="ACHKW0_001218"
     CDS             25535..26599
                     /gene="cbrA"
                     /locus_tag="ACHKW0_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance lipid reductase CbrA"
                     /protein_id="HFO7474085.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            complement(26596..27921)
                     /gene="dgoT"
                     /locus_tag="ACHKW0_001219"
     CDS             complement(26596..27921)
                     /gene="dgoT"
                     /locus_tag="ACHKW0_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474086.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAQMGYVFSAFAWLYTLCQIPGGWFLDRVTYFIAIFGW
                     SVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFYTSGQ
                     FVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKAELDY
                     IRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLTWFPN
                     YLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPIICGLL
                     ISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVFNFAG
                     GLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(27996..29144)
                     /gene="dgoD"
                     /locus_tag="ACHKW0_001220"
     CDS             complement(27996..29144)
                     /gene="dgoD"
                     /locus_tag="ACHKW0_001220"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008869 - galactonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HFO7474087.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            complement(29141..29758)
                     /gene="dgoA"
                     /locus_tag="ACHKW0_001221"
     CDS             complement(29141..29758)
                     /gene="dgoA"
                     /locus_tag="ACHKW0_001221"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HFO7474088.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(29742..30620)
                     /gene="dgoK"
                     /locus_tag="ACHKW0_001222"
     CDS             complement(29742..30620)
                     /gene="dgoK"
                     /locus_tag="ACHKW0_001222"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HFO7474089.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(30617..31306)
                     /gene="dgoR"
                     /locus_tag="ACHKW0_001223"
     CDS             complement(30617..31306)
                     /gene="dgoR"
                     /locus_tag="ACHKW0_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HFO7474090.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            31584..32240
                     /gene="yidX"
                     /locus_tag="ACHKW0_001224"
     CDS             31584..32240
                     /gene="yidX"
                     /locus_tag="ACHKW0_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YidX"
                     /protein_id="HFO7474091.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(32286..33098)
                     /gene="yidA"
                     /locus_tag="ACHKW0_001225"
     CDS             complement(32286..33098)
                     /gene="yidA"
                     /locus_tag="ACHKW0_001225"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HFO7474092.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(33213..33611)
                     /gene="yidB"
                     /locus_tag="ACHKW0_001226"
     CDS             complement(33213..33611)
                     /gene="yidB"
                     /locus_tag="ACHKW0_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HFO7474093.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(33851..36265)
                     /gene="gyrB"
                     /locus_tag="ACHKW0_001227"
     CDS             complement(33851..36265)
                     /gene="gyrB"
                     /locus_tag="ACHKW0_001227"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HFO7474094.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(36294..37367)
                     /gene="recF"
                     /locus_tag="ACHKW0_001228"
     CDS             complement(36294..37367)
                     /gene="recF"
                     /locus_tag="ACHKW0_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HFO7474095.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAQYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(37367..38467)
                     /gene="dnaN"
                     /locus_tag="ACHKW0_001229"
     CDS             complement(37367..38467)
                     /gene="dnaN"
                     /locus_tag="ACHKW0_001229"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HFO7474096.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(38472..39875)
                     /gene="dnaA"
                     /locus_tag="ACHKW0_001230"
     CDS             complement(38472..39875)
                     /gene="dnaA"
                     /locus_tag="ACHKW0_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HFO7474097.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            40172..40252
                     /locus_tag="ACHKW0_001231"
     CDS             40172..40252
                     /locus_tag="ACHKW0_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474098.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            40482..40622
                     /gene="rpmH"
                     /locus_tag="ACHKW0_001232"
     CDS             40482..40622
                     /gene="rpmH"
                     /locus_tag="ACHKW0_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HFO7474099.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            40639..40998
                     /gene="rnpA"
                     /locus_tag="ACHKW0_001233"
     CDS             40639..40998
                     /gene="rnpA"
                     /locus_tag="ACHKW0_001233"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HFO7474100.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            40962..41219
                     /gene="yidD"
                     /locus_tag="ACHKW0_001234"
     CDS             40962..41219
                     /gene="yidD"
                     /locus_tag="ACHKW0_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HFO7474101.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            41222..42868
                     /gene="yidC"
                     /locus_tag="ACHKW0_001235"
     CDS             41222..42868
                     /gene="yidC"
                     /locus_tag="ACHKW0_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HFO7474102.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            42974..44338
                     /gene="mnmE"
                     /locus_tag="ACHKW0_001236"
     CDS             42974..44338
                     /gene="mnmE"
                     /locus_tag="ACHKW0_001236"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HFO7474103.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            44581..44655
                     /gene="tnaC"
                     /locus_tag="ACHKW0_001237"
     CDS             44581..44655
                     /gene="tnaC"
                     /locus_tag="ACHKW0_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006569 - tryptophan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HFO7474104.1"
                     /translation="MNILHICVTSKWFNIDNKIVDHRP"
     gene            44876..46291
                     /gene="tnaA"
                     /locus_tag="ACHKW0_001238"
     CDS             44876..46291
                     /gene="tnaA"
                     /locus_tag="ACHKW0_001238"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009034 - tryptophanase activity [Evidence
                     IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HFO7474105.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            46382..47629
                     /gene="tnaB"
                     /locus_tag="ACHKW0_001239"
     CDS             46382..47629
                     /gene="tnaB"
                     /locus_tag="ACHKW0_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HFO7474106.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            47761..48936
                     /gene="mdtL"
                     /locus_tag="ACHKW0_001240"
     CDS             47761..48936
                     /gene="mdtL"
                     /locus_tag="ACHKW0_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HFO7474107.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIADDLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            48911..49870
                     /gene="yidZ"
                     /locus_tag="ACHKW0_001241"
     CDS             48911..49870
                     /gene="yidZ"
                     /locus_tag="ACHKW0_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HFO7474108.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
BASE COUNT        11988 a        13394 c        12773 g        11751 t
ORIGIN      
        1 cataaatttc atcacattag ccgggctgaa taccaggaat tgttagccgt ttcccgtggc
       61 gacgctgttg ccgattatat tattgataat gtctctattc tcgacctgat caatggcgga
      121 gaaatttccg gcccaattgt gattaaagga cgttacattg ccggtgttgg cgcagaatac
      181 gctgatgctc cggctttgca gcggattgat gctcgcggcg caacggcggt gccagggttt
      241 attgatgctc acctgcatat tgaatccagc atgatgacgc cggtcacttt tgaaaccgct
      301 accctgccgc gcggcctgac gaccgttatt tgcgaccctc atgaaatcgt caacgtgatg
      361 ggagaagccg gattcgcctg gtttgcccgc tgtgccgaac aggcaaggca aaaccagtac
      421 ttacaggtca gctcttgcgt acccgccctg gaaggctgcg atgttaacgg tgccagtttt
      481 acccttgaac agatgctcgc ctggcgggac catccgcagg ttaccggcct tgcagaaatg
      541 atggactacc ctggcgtaat tagcgggcag aatgcgctgc tcgataaact ggatgcattt
      601 cgccacctga cgctggacgg tcactgcccg ggtttgggtg gtaaagaact taacgcctat
      661 attactgcgg gtattgaaaa ctgccacgaa agttatcagc tggaagaagg acgccggaaa
      721 ttacaactcg gcatgtcgtt gatgatccgc gaagggtccg ctgcccgcaa tctcaacgcg
      781 ctggcaccgt tgatcaacga atttaacagc ccgcaatgca tgctctgtac cgatgaccgt
      841 aacccgtggg agatcgccca tgaaggacac atcgatgcct taattcgccg cctgatcgaa
      901 caacacaatg tgccgctgca tgtggcatat cgcgtcgcca gctggtcgac ggcgcgccac
      961 tttggtctga atcacctcgg cttactggca cccggcaagc aggccgatat cgtcctgttg
     1021 agcgatgcgc gtaaggtcac ggtgcagcag gtactggtga aaggcgagcc gattgatgcg
     1081 caaaccttac aggcggaaga gtcggcgaga ctggcacaat ccgctccgcc atatggcaac
     1141 accattgccc gccagccagt ttccgccagc gactttgccc tgcaatttac gcccggaaaa
     1201 cgctatcggg tcattgacgt catccataac gaattgatta cgcactccca ctccagcgtc
     1261 tacagcgaaa atggttttga tcgcgatgat gtgagcttta ttgccgtact tgagcgttac
     1321 gggcaacggc tggctccggc ttgtggtttg cttggcggct ttggactgaa tgaaggtgcg
     1381 ctggctgcga cggtcagcca tgacagccat aatattgtgg tgatcggtcg cagtgccgaa
     1441 gagatggcgc tggcggtcaa tcaggtgatt caggatggcg gcgggctgtg cgtggtacgt
     1501 aacggccagg tccaaagtca tctgccgttg cccattgccg gcctgatgag caccgacacg
     1561 gcgcagttgc tggcggaaca aattgacgcc ttgaaagccg ccgcccgtga atgcggtccg
     1621 ttacccgatg agccgtttat tcagatggcg tttctttctc tgccagtgat ccccgcgcta
     1681 aaactaacca gtcaggggct atttgatggc gagaagtttg ccttcactac gctggaagtc
     1741 acggaataat aaaaaagccc ggcgtcatgc cgggcaaaag tcaccagtta cgtttatgcc
     1801 actgtcaact gctgaatttt tttctcgcgg cggattttgc gttcttccat caccgccact
     1861 atcgccatca ggcagataca accaatcgcg gcgatatcaa gtgcggcgaa ggtgcctgcc
     1921 cagccggtaa ggccgaatac cggcgtccca tcggcaatca ttcccagacc taacttggca
     1981 aagctgtcac caatcaggta agcaaaggtg cctttaatac catcggcagc gccaatcgct
     2041 tttttaggta caaagccaac agcagccaca ccaatcaaca attgcgggcc aaagaccagg
     2101 aaacccaacg caaagagaga agccagatag atatattcgt tgctggcatg ttgatacaca
     2161 ccgagcgtgg cgataatcag cgccagcgcg atgcaggcca ccaggccacg gcgaccgttc
     2221 gccaggtcag agagccagcc ccacagcagc gtaccgacca gcgcaccagc ttcaaacagc
     2281 gtaaagccct gaatcgccac cgctttagag agtttcagtt cctggaacgc gtatacggtt
     2341 gaccactggt cgataccaat acgtaccaca tagaggaaaa tgttggcgaa gcacagcagc
     2401 cagatcactt tgtttttcag cacatactca acaaagatct gccacttggt catatcggta
     2461 gattctgtct ctttgtcctc ttcgctgatc tcctcgccga acagttcttc agctttgccg
     2521 aggccataag attccgggga gtcgctgccg taacgcaggc cgataaaacc gacaatcagc
     2581 gcgataatcg acgggaagat aaacatgccg atgacatggc catcgaacag gtaatttgcc
     2641 ccgaacagcg ccacacctgc tgcgcctgca ccgccaaggt tgtgagaaat attccagaaa
     2701 ccgaggaatg tcccgcgttt acgacgcggc gtccatttgg tgatggtgga gtaactgcac
     2761 gaaccgccgg tactctggaa aaagccgctt aaggcgtaga aggcaatcat caggaacagg
     2821 ctaaccgagc cgctgcccat actggcactg aagcccagca tacaaatggc agacaggatc
     2881 agcatgaacg gcaggaattg tttggtgttt ttgccgtcgg cgtagtagga aaccagcgtt
     2941 ttacccacgc cataagtgat ggagaaaccc aggccgatca tccccagctg cgtcatgctc
     3001 aacccgtagg tcgagatcat gtcgttctgc gcgatgttaa agttcttgcg aatcaggtac
     3061 atcgtcaggt agccgataaa gaccaccagg taggattgca tgaacggttt gaaccacatt
     3121 ttgcgccgca cttcgagcgg aaggtccagg gtcggcttgc gaacctggtt taagaaagcc
     3181 agcatgggtt actcctgaaa tgaatacctg cgtgaggcat gcattgtaaa aatcagcagc
     3241 aaatcaggcc tgagatagcg tccaggcaaa acctggaaaa tttcttagtt ttgccggata
     3301 tgaagtgaaa aggtgagatg catcacgctt cgcgcggtgc ctgggcgttc aaaaagggca
     3361 gtaacagcag tgcggaaatc ccggcggcga tagagataac cacaaaaaat ccgctccagt
     3421 gccaggtatc gagtactttc gccagcggcc aaccagcaag cgacgccccc agataagcaa
     3481 acaagccgac aaaccccgtc gccgcccctg ccgcctcttt gtgggaacac tctgccgccg
     3541 ccatgccgat taacatctgc gggccaaaga caaaaaaacc aatggtgaag aagcaggttg
     3601 cctgcatcac gtagctggca aatggcatca gccacagcga gccgactgaa agcaaaattc
     3661 cggcggcgaa aatcaaattc atcggcccac ggttgccgtt aaacaatttg tccgagcccc
     3721 aaccggctac cagcgcaccg ataaatccgc ccagttcaaa catcgtcact gccgtattcg
     3781 ccgtgaccag atcgacgccc agtgtctcgg acatatacaa attgccccag tcgttgatcg
     3841 ccgcccggac cacatagacc agcacatagc aaaacgaaag cagccagata tacggattca
     3901 gcaacacata tttggtgagg atctctttac gcgtcaaccc tgccccttct tgttgttgag
     3961 caatttccag cgcgtcgtgt cgccattcac cgaccgccgg taaacctaac gcctgcgggc
     4021 gatcgcgtag ccgccagcag agaaaaatcc ccacgactat cgccatacaa ccagcaatca
     4081 tcatcccggc acgccagccg taatgcagcg cagccgctgc catcacaatg ggaatgagtg
     4141 cgccgccgac gttatgcgcc gtgttccata atgcccacca accgccgcgc tcggtacgtg
     4201 aataccaggc cgttaacaga cgcgcacaca ccggtgaacc ccagccctgg aaaaaggcgt
     4261 tcagcaccca gagcacggca aacgcccata gcgacgtcga gaagccaaac agaatgttga
     4321 tgatgcccgt ggcgataagc cctatcccca taaaataacg ggcatttgag cgatcgctga
     4381 caatgccgga gacaaacttc gacacgccgt aggtaatgta aaacagggtc gctaacaggc
     4441 cgatatcgct acggctgagc acgccgttag caaggatttc tggtacggcg gcgttaaaac
     4501 ttttacgcgt gaagtaaaac agcgcgtaac ccagccagat ggtcagcaga atatgccgac
     4561 gccagtagcg atagcgggca tcaatttcat atttatcagt cattaatggc gcatcggcag
     4621 gcgctttcag aaacggcaac atcatcaaac cttagacata gcgttgaggt agagaaacgc
     4681 tgacacgcgt gccgtgcaga caggaaatgt gtaatgtgcc acccagcgcc gttacgcgct
     4741 cgcgcattcc ggtgaggcca aaaccttgtt gcccggaacc cggcggcaaa ccgctgccat
     4801 cgtcttcaat aaccagcatc aaccgttcat cctgctgcca gccttgcagc gtgaccgcgc
     4861 tggcatcagc atgtttcaca atgttgttca gcccttcctg gcagacacga aacagcgtca
     4921 cgcgctggtt ttcacttaat gctgattcat cgattcgcca ttcgagatgg ctgacaatac
     4981 cgcgcccttc cagctccatt tcccgcatca gtgagcggat ggcctgctcc agggtgagat
     5041 catccaactg gcgcggacgt aaccgaccca acaaacggcg caccgcgtcg taaacgccca
     5101 gcgatagttg ttcgatgagc tgcccgctct gcttcacgct ggcgttatct gccgccagcc
     5161 gctgaacaat gcccgcctga gtacgaatag cagtgatggt ctgaccgata tcatcatgca
     5221 gctcacgcgc cacatcacga cgcacgctct cttcggtttc cagcaaccgt tcagccagat
     5281 gctgattgcg cgccagttcc ttttgcagcg actggttaag ttcacgcaac cgctggatgc
     5341 cagcgcccag taacaaccct gtcagacttt gcaccagcaa cgagagcaat aaatccaccg
     5401 gatgatcgcg ccaggtttga ctggcgatca gcgcgatggc gttcatcaac gtcgcgatca
     5461 gcgccccttg ccaaccgtag tgccaggcca gcgcgataat cggcagcgcc aggcagaatg
     5521 gcgtaaagcg cgacagttcg tccggcaatc ccaactggag ccagagacta ataacaaaca
     5581 acagcaagta ccagaccaga tgccgcccgc gccagttgat cggttgagaa accagtgacg
     5641 ggccgagcgg cagccaggtg ttattggcga gatagtgcca gaacaccaga catatcgggg
     5701 ccagcgtcag gccgccagtt aaagtcagca acagcgcatt ccacgactct ttgccgtgcc
     5761 aaagccaggg cagcgactgc aacaacgccg ccgccgttaa cgccgccccc tgtaacagca
     5821 aggtgcgcca gtcacgctga tggcgatagc gcgagatcag cgctacgggc agtaacgtca
     5881 gtaaactacc gatcatcaat aacggaaaat gggttaaacc gaccgcctgc gttagccagt
     5941 aaatcagcag ccattccgca cccagcaata cgggccagta tccgcgcggg cattgcagca
     6001 ttagccctag ccgcagacca aacggaaata acagcaccgc catatcaggg cgctcaacca
     6061 ggtgcaggct gatactccac aggcaaaacc atgcggcaga gaagataaaa aagcaggcaa
     6121 taacggtaat taagcgggag aacaacgtct tcatcaccag ccatcaaaca tgcggcgcgc
     6181 cagttctacg tcgttactga cgcccagttt ttccatcaga ttggcgcgat ggacgtgtac
     6241 cgttttcggt gacaagccca gttcggcggc aatctctttc accgccattc cttgcgccag
     6301 tttttccgcc acctggcgtt cacgtttggt cagcgggtcc tgacgaccgg atgccagttt
     6361 aatggcaata tccggcgtca gataacagcc gcccgtagca accgtatgca ccgccgcaat
     6421 cagttcgtca gggctacagc gtttggagag aaagccgcgc gcccccgcgt taagcgcctg
     6481 ctcaaccagc gccggactgt catgaacaga gagcattatt gtcgccatac ctttcggcag
     6541 ctggcttagc agttccagac cggagatatc gggcatggag atatcgcaaa tacacacctg
     6601 cacaccgcgc cccggcagcc ccgccagcgc ctcgcgcccc gaaccaaact cggcaactac
     6661 ctgcaaatca ggttccagcc ccagcagctg cgcaaagccg gagcggacga tgaggtgatc
     6721 gtctataagg gcaacggtga tcatggtctt gtcctggcgg gtaaaaaaaa acgcgcttac
     6781 cttaacgata agcgcgatgt tgttcaagcc ttgagcggtt actgaaaaaa caccgcaatc
     6841 ttgttaaaca tagtcggatc ggactgatta cgctgcactt tcaccacatc ttccagctta
     6901 tcgatttggc ttatcatctg ctccagacgc tggtcgtcat tgaccagtag ccagatatgg
     6961 cttttgtcgc tgtcctgaat cggcagacaa agaatgcctt caacgttaaa agcgcggcgg
     7021 gcaaaaaggc cacaaacgtg ggtcattacg cccggatggt tgcgaacggt gagctccaga
     7081 atcacgttgt catgagttgt gttttgcatg gcttattccc ccaccatttc agtattcgcc
     7141 gcacctggcg gcaccatcgg gtaaactttt tcttcggcat caatgcgcac atggatcagc
     7201 gccgggccag ggcgattgat gatttcctgc aatgcagcct gcggatcggc ttcgttattc
     7261 aaatcacagg tttcgaggcc gaatccggcg gcaatctgca taaagttgat ttttcccgga
     7321 taggtggcgg caaaaacgcc ttgctcgtag aacagactct gttgctgatg caccagcccc
     7381 agcgcttcgt tgttcatcag aatgattttg acatccagct gattttcact ggcggtcgcc
     7441 atctcctgaa tattcatcat caggctgccg tcgccggaga aacacaacac tttgcgatcc
     7501 gggttcgcca gcgccgcgcc aatcgccgca ggcaggccaa aacccatcgt gcccagccca
     7561 ccggaggtca gccactggcg tgggcgattg agcggataag cttgcgcggt ccacatctga
     7621 tgctgaccca catcggtggt gataatcgca ttgtcatcga cacaggcggc aacggcgttg
     7681 atcaggccgt aatggcttaa tggatcgcac gctttcggga ttggacacgg gaattcgcgc
     7741 tgcaaatccg ctaccaactg gtgccactct gcacgcggtt gcgcttccac ctgcgggatc
     7801 aattgcgcca gcacgtcatc aacatccgcc tgaatcgcca catgcggctg cttgatttta
     7861 cccagctctg cgcgatcgat atcgacatga ataattttgg cattcggaca gaactgctcg
     7921 gttttgccaa tcgcccggtc atcaaaacgc gcaccgagca ctatcaacaa atccgcctcc
     7981 tgcaaaatat agttggtgct gcgcacgccg tgcatcccca gcatacccag cgacaacgga
     8041 tgcgcttttg gcaacatgcc cagcgccatt aaagtcatgg tggtaggcag ttgcgctttc
     8101 tccgccagtt cacgcacccg tgcgggcgca ttgatcacac cgccgcccag ataaagcacc
     8161 gggcgtttgg cagcgttaat catcgccgct gcgtcacgaa tgctttcttc gctaaaggcg
     8221 ggggcggcgg ctttttctgc catagcgggc tgtgtttcaa tctcaaaaac tgccgtttgc
     8281 acatccttag gaatgtctat ccacaccggg cctgggcggc ctgattgcgc aatgcggaag
     8341 gcatcgctca tgacctgcgg gagttcttcg atatgtctga ccagatagtt gtgtttggtg
     8401 atggggatag agatgccgta ggtgtccact tcctggaagg cgtcggtgcc gatcatcgag
     8461 gcgggaacct gaccggtgat gcaaatcagc gggatggagt ccagccgcgc atcggcaatg
     8521 gcggtcacca ggttagtcgc acccggtccg ctacaggcca tacagaccgc cggtttaccg
     8581 tcggtgcgcg ccatcccctg agcgataaat cccgcgccct gttcatgacg ggccagaata
     8641 tggcggattt gcgtgctttg gcttaaggca tcgtaaacag gcaggataga accgcccgga
     8701 atgcccgtca caatcttaat gccctgctgt tccaggaaat gaacgataaa ttctgcgccg
     8761 gtaaagcgct tacgcgtcga tgttgtgccc gaacttgcca tgctccagtc cttttcttct
     8821 gggccgactt tccgggaggt gcttaaacga aaaaccccgc ccggtttgcg ccggcggggt
     8881 tttggaatcg tgtgttgttc cagtccctac ggcgcattgc cgacgaccac caccacacgc
     8941 acgacgacca ctgcggcgga tagcgcagtt ggtagtagtt ttgcgttgaa catggaagta
     9001 gtcattgggg accttgttgg ttttgtgttt aacaatattt atacaagcac agctttacag
     9061 gggagacaat ggaaaatttt tcagcaaggg aaaattgagg ggctgatcac gttttgtact
     9121 gaattgcaga taacaaaaaa ccccgccgga gcgaggtttc atcagccgcc tgcggctggt
     9181 aaccgcaaag cacactgtat tatgtcaaca ctgaaagtat acgtgttccg cgcagaacgc
     9241 gcaatttcgg cacgaatttt gacgtattta gggcatagtt gagtatcgat cacagtttgc
     9301 gttttgtcca aatattactg tttatttata cagtaaactt ctataatatc actgtacgca
     9361 atgtgttatg cgggggccgc atcgttaccc ggcgcactaa gtcctggctg aaacgggtgg
     9421 tgccgtcagc gccttaaccc cgcgtgagca cactgtgtta tgtcaacaag cacaacgttt
     9481 ctccttaaga taccgcgtgc acaacagctg gcaacaggca gcggaaaggt acgtcagctg
     9541 gcagtgctcc tgaaccacag gagacgcgta tgagcctggt ggatatcgcc attcttatcc
     9601 tcaaactcat tgttgcagca ctgcaactgc ttgatgctgt tctgaaatac ctgaagtaat
     9661 tcagattcaa gtcgcaccaa aggggagcgg gtaaccgctc cccttttata tttagcgtgc
     9721 gggttggagt tgggggcttc ttctaccagc agaatgtttg cacctgcgct gcctgtgccc
     9781 ctcaccctag ccctctcccc aaaggggcga ggggaccgtc ccgtgcaatg tttagctcat
     9841 tttctgaaag ggaacggtgg tttccccctc gcccctttgg ggagagggtt acagttcgca
     9901 cgtaatccct cccggcaaca tttattgccg cttttgttta catattctgc cgctaaacaa
     9961 ttctccattc ctggcgtata tctggctaac attcatcaat gtgatagatt cctctcccgc
    10021 atttatggga atgcgtagtg acttattcta attattttta taaaagcatc cgtgataatg
    10081 aaaaggcaaa gaaacgtcaa tttgttattg atgttggtat tactcgtggc cgtcggtcag
    10141 atggcgcaaa ccatttatat tccagctatt gccgatatgg cgcgcgatct caacgtccgt
    10201 gaaggggcgg tgcagagcgt aatgggcgct tatctgctga cttacggtgt ctcacagctg
    10261 ttttatggcc cgatttccga ccgcgtgggc cgccgaccgg tgatcctcgt cggaatgtcc
    10321 atttttatgc tggcaacgct ggtcgcggtc acgacctcca gtttgacggt gttgattgcc
    10381 gccagcgcga tgcaggggat gggcaccggc gttggcggcg taatggcgcg tactttgccg
    10441 cgagatttat atgaacggac acagttgcgc catgctaaca gcctgttaaa catggggatt
    10501 ctcgtcagtc cgttgctcgc accgctaatc ggcggtctgc tggatacgat gtggaactgg
    10561 cgcgcctgtt atctcttttt gttggttctt tgtgctggtg tgaccttcag tatggcccgc
    10621 tggatgccgg aaacgcgtcc ggtcgatgca ccgcgcacgc gcctgcttac cagttataaa
    10681 acgcttttcg gtaacagcgg ttttaactgt tatttgctga tgctgattgg cggtctggcc
    10741 gggattgccg cctttgaagc ctgctccggc gtgctgatgg gcgcggtgtt agggctgagc
    10801 agtatgacgg tcagtatttt gtttattctg ccgattccgg cagcgttttt tggcgcatgg
    10861 tttgccggac gtcccaataa acgcttctcc acgttaatgt ggcagtcggt tatctgctgc
    10921 ctgctggctg gcttgctgat gtggatcccc gactggtttg gcgtgatgaa tgtctggacg
    10981 ctgctcgttc ccgccgcgct gttctttttc ggtgccggga tgctgtttcc gctggcgacc
    11041 agcggcgcga tggagccgtt ccccttcctg gcgggcacgg ctggcgcgct ggtcggcggt
    11101 ctgcaaaaca ttggttccgg cgtgctggcg tcgctctctg cgatgttgcc gcaaaccggt
    11161 cagggcagcc tggggttgtt gatgacctta atgggattgt tgatcgtgct gtgctggctg
    11221 ccgctggcga cgcggatgtc gcatcagggg cagcccgttt aagcgcacgt caccgcagca
    11281 tcgtcatcag ctccatggga gaacgatgct gctttatcag atcacgcatc acccgcatat
    11341 gcggtgcgga gtaagaataa aacgcctgat agcccgcaca aagcacgctg tctggcgtct
    11401 cctggcgagc gtgcgggcac aggcgatgcc agtcgcacgc ctggcagtca cggcgcggtt
    11461 gcgggttttc gctgccacac catttttgta aggtggcgtc aaaaagctca atcgagatac
    11521 gactgatatc tgcccgcacc caacactcga acagggcaat taaaaatttc ccccacagct
    11581 ctgctgtcac cgaagcggcc gtcagatcgc cccggtcatc aaattcaaga tagggaatga
    11641 gctgtacgga gcggatgccc cgctgtttga aattgtgata aaaatcggca ggttgcagag
    11701 catccttgta ctctacaacc agtttatgcc tgtcctcttc agcatcaatg acctgacttc
    11761 ctgtcatgcg actctctcaa taaatacgat aagttgatgt atatgcgtta cagcaaacag
    11821 tattgcgaga gataacaccg cgagggagat caaaaatttg tcacggacta cgtgaaattg
    11881 ggaaaaatcg ctattcgtga catccattaa attgcgatta cgggtgtagt gccagaggat
    11941 cagcgccacg atggcgagaa tgccaatcga aatccagaat aacatgcccg cctggtgcca
    12001 gttgtgtttg atagccaacg ccatcaacgc gccgtagccc agcatggtac gaaaccacgc
    12061 cagtgatgtg cgctccggct gcagtcctgg gtcggcaatg cgtctggctt tgcggctatc
    12121 cggcatacaa caccagtccc ataacgatca ccgccacgac catcaatatt aagctaatga
    12181 ttaataagct gttggtgtag ggcaggtcct ctttcagacg catcgctttt tcattgcgta
    12241 accagcgcag gtagccatac atcgctaacc caccggaaaa tagacacaac aacaacgcca
    12301 gcagttcgcg aatgacgggc gtggcgaaat ctggcgcaag ttggtcaagc ccaacccccg
    12361 ccgccagaaa tcctagtgcg gtacggatcc acgccagaaa agtacgctca ttagccagcg
    12421 agaagcggta atcgggcgct tctccgaggc gggaaatttt cattgttgct cctttgcaat
    12481 ttcactaacg ctctatcttt agcggttttt tttaagaatt tcgtccgccc gtttcctgat
    12541 atgcttttgc catcccctac aaggaggtca gatgggtatc atagcgcaaa ataaaatcag
    12601 ttcattgggc atgctgttcg gtgccatcgc tttaatgatg gggattatcc acttttcatt
    12661 cggccctttt tccgcaccac cgccaacgct tgaaagcatc gtggcggaca aaacggctga
    12721 aattaaaaga ggtcttctcg ccggaataaa aggcgaaaaa attactaccg tggagaaaaa
    12781 agaggacgta gatgtcgata aaatcctcga tcaaagcggt atcgcactgg ccattgctgc
    12841 gctactttgc gcatttattg gcggtatgcg taaggaaaat cgctggggca ttcgcggcgc
    12901 gctggttttt ggcggcggga cgctggcttt ccataccttg ctttttggta tcggcatcgt
    12961 gtgcagcatt ttgctgatat tccttatctt ttccttcctg actggcggtt cactggttta
    13021 agcagcctga ataatgctcg ccgggtgttc atcagcgggc attatcgtca gaacttctgt
    13081 tttttctctt ccactttcac tccttgcgtc ggtaggccgg tgtcatagtc acgtaccacc
    13141 ggcgaatagc catcttgtgg gcgtggacga aatgccccca tccagcgcgg cagtgcatct
    13201 ttacgccacg gacgcaggct ccattggtaa ctgcggaacg gatcgcgaat tttgtccatg
    13261 taatccaata aggcgtcatg cattttgctg cgaacgtctg caaaacggat atcatcgatc
    13321 aggttatgca tttcatttgg gtcattacgg cgatcgtaaa gttcatcact ggtgaagagg
    13381 ttgagtacca gtttaaagtc atccgtcacc cagcaacgca ccggaataaa accgccaaag
    13441 ctgtcatgct caatctcgta gcggttaaat tccaccatta caccgcgtgg ctctttcacg
    13501 gcaaggatat tttcccccgg cagaatctct ggtttttcaa tatctgccag cgccatcatt
    13561 gtcggcagta aatcgatatg actgactggc gtatcgacct gtcgccgctc cccttgcggc
    13621 gaacggatga tcagcggaat acgggtgatg tcgtcataca tcgccgctcc tttactgatc
    13681 agcttatgtg cgcccatcat ttcgccgtga tcggaagtat aaatgaccca cgtattttca
    13741 cgttgctctg gcgttaaggc attgatgacc cgtccgattt ggtcatcaac aaagtcatta
    13801 caggcaaaat agagcggatg gtgataaagc ccgtcatcac cgactggcga tggcatcgcc
    13861 tgcgcccata agcggtgatg ttccggtttg ttcgccaggt catcctgagc tttctcgccc
    13921 agatcgtagt aaaaatcagc gtatttctct aaatactcca ccggacaggt gaacgggtga
    13981 tgcggctcat catacgaaac caccatcagg aagggttcct cggcgcgcgc gggctgttgc
    14041 agaaaatcta ccgcccgatt gctgatgcgg tgcgcccagg tgaaggtttc gtcgatatgg
    14101 ttcgcctgta aatcctcaac gctgtttagg ccattgcgcc acaggctgat ctctttttcc
    14161 gtcagttcgc taaggtagtt cgccccatcg aaccagtaat cagcgtccca ctccggcgga
    14221 cactcgccag tgccgaaata gtcatgaccg tcgagatgcc atttgccgat gtaacaggtg
    14281 tgatagccgg catccttaaa gtagcgcccc atagtggaga tgtttttgcc tggcgcgacg
    14341 ttgttggtcc acgggccgga ctggttagcg tagataccgg taaatagtcc ggcgcgtgca
    14401 ggcgtacaaa ccggtgaaca ggtgtaggcg gaattaaagc gaataccttc cgccgccaga
    14461 ctatcaatat tttgcgtatt cagcggttta ccgctatagc aaccgaccat attggtggcc
    14521 tgggtatcgg tcatgatgaa cagaaaattg gggcgtttca taggctatcc ttcgcgtcgg
    14581 gagtaaaggt tacggctggg tcgtgacgat gccgccagct gtcaaaaatc aggtagacaa
    14641 tcactacggc ggcaatgaaa taactgatca tcgataacca gcgcgtaccg tagccgccga
    14701 attcagccag cccggcatag acgccaatca tcgcgaacag gatgccaatt gacgcgatct
    14761 tgacgtttct ccacggtttc atgtccaccg caaacgcatc tttgaaggtg aacggcgtgg
    14821 agcgcggttt gataaaaccg atcaccagca tcacgaccac gttgatgcag aacgtacagg
    14881 ccagcacata gaggaaatgg aagtcgaact tcaccagata gttgatggtg atgtagctga
    14941 ttatgccaat ccccatcgcc acttttgccg ccagcgccgg gatgcgcggg aagaaaaagc
    15001 ccataatgat gatggtaacc agcggcacgt tgtaaatgcc gttgagctgt ttcatccagc
    15061 tatacagccc ctgcggcgcg ttggcgatcc acggcgcgac cagcaccgaa acgatagcga
    15121 taaagaaacc aaatttgcgc ccgacggtga ccagctgctg cggctcggca ttctggttaa
    15181 tgatgcgacg gtaaataccc atactgaata acgtactggc gctattcaga aagccgttga
    15241 aggtactgat caccgcacca aataacaccg cgccgaagaa acccaccatt ggcactggca
    15301 gaacgttatt gaccagcgtc gggtaggcca tgtcggcttt cggtaaatcc tgatacagat
    15361 gaaacgcaat caaccctggc agcaccagta ccagcgggtc gagcattttc agcaccgccg
    15421 ttaacagcgc ccccttttgc ccttccgcca ggctttttga cgccagcgtg cgttgcacga
    15481 tgccctgatt tgtacaccag taaaaggtgt tcaccagaat caaaccggta aatgccgcgc
    15541 caatcggcaa gggatcggtc gggccaccga ttgagtttaa tttctcggcg tgaacggtgg
    15601 tgagttgctc aatgccctgc ataaagctgc ccttgcccat cgcgatcagg ccaaataccg
    15661 gcaccatcaa cccgccgata accagcccaa taccgttgat ggagtccgcc actgccattg
    15721 cgcgcagtcc gccgatcacc gcatacaaaa ttcccgccag accaagcaaa attaccagta
    15781 gccagatagc cgcaccgtga gaaatctgta gcgattcccc gacgtgaaac aggctgttga
    15841 gcgccaacgc gccggagtag agaacaatcg gcagaaagca gacgccggtg gcaatgagga
    15901 agcagaagtc gatgataata cgcgtcgttt tatcataacg ttcctccaga aaatcgggga
    15961 tggtggcaat gccgcgcttc agatagcgcg gtaaaaagat tagcgcgagg aagatcagcg
    16021 tcaccgctga agtcacttcc cagcccatca ccgacatgcc gcttttgtag gcctgcccgg
    16081 aaaggccgac cagttgttcc gtggaaaggt tggttagcat taacgaagcg gcaataaccg
    16141 gcgcttttag tgaacgtccg gcaagaaaat agccttgttg cgatccggta tctattttgc
    16201 ggaccttcca ccaggtgatc accgccacca gcagcgtaaa accgacaaaa ctcaagattt
    16261 gtaacgaatt catctcatag cccttattta tgtttatgtc ccggacgcat gaatataggg
    16321 agaggtaaaa tgcgtcgggt caggcagtca gattcttaaa atacaaacgt cgtatccctg
    16381 aacggattgt tttctgttaa gttcaggttg tgagcataat tcgccggagg gatattaaaa
    16441 tgaatggaaa attgcaaagt tcagatgtaa aaaacgaaac tccgtacaat attcccttat
    16501 tgattaacga aaatgtgatc tccagcggaa tttctctgat ctcgctctgg catacctacg
    16561 ccgacgagca ttaccgggtg atctggccgc gggacaagaa gaaaccgctt atcgccaact
    16621 catgggttgc ggtgtatacc gtacaaggat gtgggaaaat tcttttaaag aatggcgaac
    16681 aaataacact gcatggcaac tgtattatat tcttaaagcc aatggatatt cactcttatc
    16741 actgtgaagg tttagtctgg gaacagtact ggatggaatt tacccccacc agtatgatgg
    16801 atattcccgt tggtcagcaa agcgttattt ataatggcga aatttataat caggaactca
    16861 ccgaagttgc tgagttaata acttcaccag aagcaataaa aaataatctg gcagtcgctt
    16921 ttctgacgaa aattatttat cagtggattt gtcttatgta cgcagatggt aaaaaagatc
    16981 cacaacggcg gcaaattgaa aaattaattg ccactttaca tgccagtctg caacaacgct
    17041 ggagcgtagc tgatatggct gccacgatcc cctgtagcga agcctggttg cgtcgtctgt
    17101 ttttacgcta taccggcaag acgccgaaag aatattacct cgatgcgcgt ctggatctgg
    17161 cgctatcgct attaaagcaa caaggaaact cggttggcga agtcgctgat acgctcaact
    17221 tcttcgactc ctttcatttc agcaaagcct ttaaacataa atttggttat gcgccgtcag
    17281 ccgtgctaaa gaatacggac cagcacccaa cggatgccag tccacacaat taacgtaact
    17341 ctggccagta cgctttgttg gcctccagca actcatccag aatagctttg gcgaccgagg
    17401 cgctcggtac cgttttcgac aacgtaatcg cctaccacag gtgctgatat gaacgctgtt
    17461 cccaggcgtc cacgaccagt ttttccaccg ccacttgctg actcattaac cctttctgaa
    17521 actgcgggat atcgccgacc accagtggtt caggccgtag tagcgcacgc gcatctgttt
    17581 gcgatcttgc agcccaacaa tttgcgccat ccggctttca ataccgattg gcatgtcaca
    17641 gatgttgagg attttcgcat tcgggcgcag acgacgcgtg gcttctgcga caattgctgc
    17701 cgggttggag tagttgagca tccaggcgtt tggtgaatat ttttccatat aatccaccag
    17761 ttccaggacg ccgccaatgg aacgcatgcc gtacgctatt ccgccgggtc cgcaagtttc
    17821 ctgaccaaca acaccgtggc gcagtgggat tttttcatcc agttcgcgca tcgggtactt
    17881 gccgacgcgg atgtgcgcca tgacaaaatc aacgtcgctg aatgccactt caggatcggt
    17941 ggtgtaacta aacgcaatgt ccggcgcttt ctctttaagg atgactttac aggcttcggc
    18001 aatcacttcc tgccgcgcac catcgttatc ataaaatttc agtgcacgaa gcgggaaacg
    18061 gtcctgattc gccaggagca tcaacacgat gcctggcgta aaggtgcttc caccgcctgc
    18121 gacaaccact gagaatttgg tcattatact gcctccgtaa tggcaacatt ttctgctgat
    18181 tgatgagaat taattaagct atcgagctgt tcacgcagct gggatacatg cagaccaatg
    18241 atcacctgaa tggcatcgcc actacggaag acgccgtgcg ctcccagctt tttaaagact
    18301 tcgtcatcca gcgtttgtga catgtcatgc agtgcaatac gtaaacgcgt cgcgcaattg
    18361 ttaatgctgg agatattgcc gaccccgccc agggcttgca ggataccggc agcctgatcc
    18421 agctcttttt ttggctctgc cgcggtggtt tggcctcgcg aggctttgta ttcggctttt
    18481 gagtagagtt tcacttccgc atcttcacgt cccggcgtgc acatgttgaa ctgcagaatc
    18541 agtgtgcgga aaaccacgaa gtacagcagg gtaaagcaca acccaatggc gatttgggtc
    18601 agcatcatat ccgcgtggtt gctgaacatc gggatccagt tttgcggtaa aacctggtca
    18661 atcagacctc cgcccatgtt gcccaccaca ccaaagagat acattacggt cgacattgag
    18721 gccgccagca cggcgtgtac cgcaaacagc aacggtgaaa tgaacaggaa ggtaaattcc
    18781 agcggttcgg taattcccac cagcatggcg gttaaggttg cgggaatcag caagcccgcc
    18841 acttttaccc gattttccgg tgcggcagtg aagtacatcg ctaaagaaat gcccacggca
    18901 ccaaagattt ttgagttacc gtgcagggca aaaccgcctt ccgggaacaa cgatttcagc
    18961 ggctcggcgc tcaaactgaa ctcttgcaga tgctgcgccc agtacatctg aatgccgcct
    19021 tcaacagctg ccggaccaaa gataaactgt ccgtagatga agtggtgtaa accggttggg
    19081 atcagaatac gttcgaggaa ggtgtaaacc cagaccccaa gtgcacccgc cgaacgcagg
    19141 aacgcttgca gagattcaat ccccatttgt acttttggcc agccgagcaa cgtcagccag
    19201 gcacagggga tcatcaccag gaaggcgata atcaccacat aagacgtccc ctggaaaatg
    19261 ccgagaaaaa ccggcagttt tttatcgaac agacggttat gcagcgccgt cacaatgccg
    19321 gaaatgataa ttgcgccgat aatgctggta tcgagggttt taatcccagc catcattgtc
    19381 agaccgctac ctgccaccgc gtcctgagtg aaatcgacgc cgaagtagct tccccaggtc
    19441 attcccatcg cattgatgaa atagttccag gtcaggaaac tcaccatcac cgccagacaa
    19501 gcacgtccct gcgcttgctt agcaaggcca atgggtaaac cgacagcaaa aatcagcggc
    19561 atattacgga ataccgtcca accgccctct tcaataatgt gtacgatttg cgcgaataaa
    19621 ctgttcggat cggtcagtga ttccccgaca aacatcgggt tttgcagcaa gatggcaaga
    19681 cccaccacaa tcccggcgaa gggaaacagc agcactggcg tgaacatcgc gccgccaaag
    19741 cgttgaattt gactgagcat ttccacatcc tcttttctca attctgtaag taggaagcct
    19801 gattggttat gcggcaggcc tggagcgagg ataggaagat gcgatcggca gaacatgacg
    19861 acccaatggg attcgtgatc gctttcatgc ttttcgttgg gcggtagaga tctacgtttt
    19921 cgcgaaaaag tagacatctg tgagcactgg tacacagcat cgaacacatc tttaaaaaaa
    19981 agatgttttt tcaatcgatt aagcagaact tgtgggcgca ttacccgggc ttgcaggcaa
    20041 aaaagagatc tagagatgat ctacaaaagc attgcggagc ggttaagaat tcgacttaat
    20101 tacgcggatt tcacgctaaa cagccttctt cccggtgaaa aaaagttggc ggaagaattt
    20161 gcagtatccc ggatgaccat ccgtaaagcg attgacctgc tggtagcgtg ggggctggtg
    20221 gtccgccgcc acggcagcgg cacttacctg gtgcgcaaag atgtgctgca tcaaaccgcc
    20281 agcctgaccg gactggtgga ggtgttaaaa cggcagggaa aaacggtcac cagccaggtg
    20341 ctgatttttg aaatcatgcc tgcgcctccg gccattgcca gccagttacg gattcaaatc
    20401 aacgagcaga tctacttctc ccgtcgcgtt cgttttgtgg aagggaaacc gctgatgctg
    20461 gaagacagct atatgccggt taaactgttc cgtaatcttt cgctgcaaca tctggaaggg
    20521 tcgaagtttg aatatattgg acaagagtgc gggattttga ttggcggtaa ttatgaaagc
    20581 ctgacgccgg tgctcgccga tagactgctg gcgcggcaaa tgaaggtagc ggaacacacg
    20641 ccactgctgc ggatcacctc gttgtcatat agcgagagcg gggagttttt gaattattca
    20701 gtgatgttca gaaatgccag cgaataccag gtggagtacc atttacggcg actccacccg
    20761 gaaaagagtt aaccgatact ccagaagagc accgccagta attggggggt gataatgcgc
    20821 agaaacatca ctaacggata gacagtggcg taagagagcg ccgccgcacc actggttgga
    20881 tgaagattat tagcaaacgc cagcgccgga ggatcggtca tggaacctgc cagcatcccg
    20941 cacatggtca ggtaattcat tttggctaac atccgcgcca gaatgccaac agtaatcagc
    21001 ggaacggcgg tgatcagggc accataacca atccagctta gcccttcgcc attgaccagg
    21061 gtattcacaa aatccccacc agatttcaga ccaacgaccg agaggaacag cacgatcccc
    21121 agctcccgca gcgcgaggtt ggcacttggc ggcataaacc agtacagctt gccgatactg
    21181 ccgatacgcc cgaggatcaa cgccataatc agcggaccgc ccgccagccc cagtttcaac
    21241 gcggccggga acccaggcac aaagacggga atagaaccta acaatacgcc aagcccgatg
    21301 ccaataaaca ctggcagcat ctgaacctgt tgcagttttt gctgcgcatt ccccagcaca
    21361 ttggcaacgg catcaattgc ggacggacgc cccaccagat tgaggatatc gccgaactgc
    21421 aggctgatat cgccactggc gaccagttcg accccggcac ggttcaggcg cgagataaca
    21481 acgtcatagc gttctttaaa gtgcaggtcg cgaatacgtt ttccgagcac gttttcattg
    21541 gtgaccacca cacgctcgac gcgcaaatca gtgcctttcg tggacagcga agtatcgacc
    21601 tcctgaccaa tcaccagttg cgcattatgt aaatccgctg gctgacccac cagatgcagc
    21661 aaatcgccca gttgaataat ggtatctggc gaaggaactt ttagggtttc ttcgcgtttc
    21721 agacgcgagc agataatttt gtcgccgttg agaatcggta catctttaat ggctaaatca
    21781 tgcaggttag ggttctcaac gcgaatattg atagtcttga tcagcgcgcc gccattggtg
    21841 cgtgaagact cgtgctgctg agcttctgtc tcgacattga cgcggaaaat aacccgcaac
    21901 atccacatgg tgaacaaaat cccgcaaatg ccgaatggat acgccatcgc gtaactcatc
    21961 cccatctgat cgaccatttc cattggtgta cccaggtcgc gcaaaatctg ctgccctgcc
    22021 cccagcgctg gcgtattggt aaccgcaccg gagaaaatcc ccagcactac cggcagtgga
    22081 atatcaaaca gtttatgcag gatggcggta accagaccac cgatgatgac gatcagaaca
    22141 gcaaacaggt tgaggcgtaa tccggagacg cgcaatgagg caaagaagcc cggccctacc
    22201 tgaatcccga tagtataaac gaacaggatc aggccaaatt cctgaataac atgcagcata
    22261 tcgctactta atgtcatccc cgcctgagaa acaaaatggc cgacgatgat cccaccaaac
    22321 agcacgccgc caatacctaa tcctatgccg cgaaatttga cattgccgat aaacaaaccg
    22381 acgactgcca ccaaagccag aatactgacc gttaatgcta tatcactcat cgtccatttc
    22441 tcttgagata acatttttaa ttgctacgga ttatggctga atcggtgaag cagatttgcc
    22501 ttatagcgca cattatcacg ggaaagaacg tgccgaaata tcttaaacag tcgaaattta
    22561 acgcattgtt gagtaagtaa gacaaaaaaa gccccaccag aatggcgggg caaagagaat
    22621 agctagttag ctatttaacg cgggacgttc gctgatagcg atacgctgcg ctgcgatggg
    22681 ttcaggctca ttacgaatta aatcaatatg cagtaaaccg tttacgaagg ttgcgccaga
    22741 gacttccata ttttcagcca gcgtaaagct caggctaaat ggctgattca taagcccttg
    22801 atgcagccat tttttctctt cttttggctg ctccggcgtg ccttttacgc tcaggcgcgt
    22861 accttccagt tgaatctcta aatcttcctg acggaaacct gccagcgcaa gggtaatgcg
    22921 gtagtggtta tcgtcgcttt tctcaatgtt gtacggcggg aagctctggc tttcaccggc
    22981 gttttgcagt gcgttggcca gtttgtcaaa accgatccat tgacgcatca gtggggataa
    23041 atcgaagtta cgcatagtca tttctccttc taagaagcga gtaagtacct gcaaatccga
    23101 agattcgcat atgctccctg acggcgagca tggagatgtc aggccgcgcc aggcggcctt
    23161 agggaattag ttgatttcga tacggcgcgg ttttttcgct tccggaatca cgcgttcgag
    23221 atcgatatac agcaaaccat ttaccaggtt agcaccacga acatgaatgt tctcagctaa
    23281 ctggaatttg cgttcaaagt tgcgttcagc gatgccctgg tacagatagg tgcgctcttt
    23341 ttgttcgtcg gcgtgagcac ctttcaccac cagcagatta tcctgggcgg taatttccag
    23401 ttcgctctca gcaaaaccag ccacagcgat agcaatgcgg taatggtttt cgtctaccag
    23461 ttcaacgtta tacggagggt agccgccatt actctggctc tggttgtttt ctaagtggtt
    23521 aaacaaacgg tcaaatccaa tagcagaacg gtaaagcggg gataaatcaa agttacgcat
    23581 aatcaatagc tcctgaaatc agcgagaatg taagaccttc cacaatggac aggtcaggta
    23641 gccagaacac ccatcaggcg cgtcctcatc ggctacgatg taaaaatggg tctggaaatg
    23701 aacttttcaa gccttatcag cggacttttt tttgcagttt atggtctatt gcatagactg
    23761 agggggcagc acgaaacgtt aaagtgcgat agccgccaca gtgcgacgta atgatgatgt
    23821 tattttcagc aagggatcgc tataactcat caggccaaca cccacgatca acagatgaat
    23881 ttattatgat aagaaatgtg ttgttagcgt tcatgatatg cagcggaatg acattactcg
    23941 gaggatgctc cagcgtgatg tcccataccg gagggaaaga aggaacgtat cccggcaccc
    24001 gcgccagcgc aaccatgatt ggcgacgatg aaaccaactg gggcactaag tcgctggcga
    24061 ttctcgatat gccgtttacc gctgtgatgg acacgctttt actgccgtgg gatgtgttcc
    24121 gcaaagacag ttcggtgcgc tcacgcgttg aaaaaagcga agccaacgcg caagccacca
    24181 atgcggttat tccaccggcc agaatgcctg ataactgatt tagcgatcag tttcggtgat
    24241 ccacagctgg ccaccttcca tccacgccag ccattgacca tccggcgaga agaccacggc
    24301 gtccgcagaa ggtggattgg cgtggttttc ggttaaatac tcaacctcgc cgctttgcgc
    24361 atgggcacaa gcaattcgat tatccagcac aaagcccaac cattctcctg acggatgcca
    24421 gttaaatgca gactgaatat ccgttttgtt atgggttaac tggcgcggct cgccgccctg
    24481 tggcgagata agccacagtt gcacaatgcc gttatcatca cgcattaaaa acgcgatttg
    24541 cgtaccctgc ggattacagc gcacccagtg gcgcgggacg ttgactaacc ccggataagc
    24601 ccgatggtgg gtaaaggtta aacgtcgctg cacgacgccg cgcggtggcg cgggcagggt
    24661 ggtttccgtt ccacttaacg gcgcatcacc tgccgctttc cagccagctt catcttgcgg
    24721 taactcaacg ataaacagct ccggcacttt ctcgcccttt ggcgaaagtg tgtcgccaat
    24781 aaacgccagc gcgtgatttc ctacccatcc ttcttcataa gcacgattga tttcatcgct
    24841 gccaggctgc ggcgtgggcg tggttttact caccagcacg caccagtggc taccgctgta
    24901 ttcacgcgga tgctgctttt gtacgttgac cgggccaaac ggcgcagcaa caccgacgtt
    24961 tcgcaaatcc agcgccggat cgagttcatg cattacatgg tcgttatagg taaagctcac
    25021 cctttcaccg ttcgggctaa agacatgcac atggctgccg ccgcgcagcg ctcctggcgt
    25081 atacggcgcg gtaatatcca ttgcatcgag attgctcacc ttgccgcctt cagcaatgac
    25141 tccgcgtcga tgatggaaat cgtaatgcca tgtttcatca ggattttccg ggccatggat
    25201 gaaaacatat ttctctgact tcggatgaac ggtcaccacg ccgacgtgtg cgccctgtga
    25261 cgcgcgatag ataacctcga cctcgccggt atggatattc acacgctcaa tggtctcgcc
    25321 ggtaaacgac gccccagaag gacgcacgtc aaataccagc cactggctgt cgggcgtcca
    25381 ggtattggta ttggtgagca ggtgattacg gggagcaaag gttatctgtt tcatggcgcg
    25441 gtcctgatac aaaacgggtc ccaccatcat acttcacaag gacttcactt tcagacaata
    25501 gtcttttgcc atcgtgatga atgtgagtga aaagatggaa catttcgacg tggcgattat
    25561 tggcctcggc ccggcaggat cggcgttggc gcgaaaatta gcaggcaaaa tgcaggtgat
    25621 cgcgctggat aaaaagcacc agtgtggtac tgaaggtttc agcaaacctt gcggcggtct
    25681 gctggcaccg gacgcgcagc gatcttttat tcgcgatgga ctgacgcttc ctgtcgatgt
    25741 gatcgccaat ccacagattt tcagcgtcaa aactgtcgac gtcgccgcat cgctcacgcg
    25801 taactaccag cgaagctata tcaatattaa tcgccatgct ttcgacttgt ggatgaaatc
    25861 gctgatcccc accagcgttg aggtttatca cgatagcctg tgccgaaaaa tctggcgtga
    25921 ggatgataaa tggcatgtca tttttcgtgc agacggctgg gagcaacata ttaccgcccg
    25981 ctatctggtc ggtgccgatg gcgcaaactc gatggtgcgg cgacatctct acccggatca
    26041 tcaaatccgt aaatatgtcg ctatccagca gtggttcgcg gagaaacatc cggtgccgtt
    26101 ctactcctgc atctttgata attcgataac taactgttat tcatggagta tcagcaaaga
    26161 cggttatttt atctttggcg gtgcttatcc aatgaaagac ggtcagacgc gtttcacgac
    26221 gctgaaagag aaaatgagcg cctttcagtt ccagtttggt aaggcggtga aaagcgaaaa
    26281 atgcacggtg ctgtttccct cacgctggca ggattttgtc tgcggtaagg acaacgcctt
    26341 tctgattggc gaagcggcag gatttatcag cgccagttcg ctggagggga ttagctatgc
    26401 gctggatagc gcagacattc tgcgtgcggt gttactgaag cagccagaga agctcaatac
    26461 cgcttactgg cgcgccaccc gcaaactgcg tttaaaactc ttcggcaaga tagtaaaaag
    26521 ccgatgcctg accgcaccgg ctttaagaaa gtggattatg cgcagtggta tggcgcatat
    26581 tccacagttg aaagattagc caacgcgctt cacatcgccc accagcagga tgtaagagag
    26641 cgcgccaatc aacgcgacgg cggagatata aaccagtgca ggtgcgaaac cgtaaccctg
    26701 cgccaggtac cccaccacca gcggaacggt gatgccgccc agaccaccgg cgaagttaaa
    26761 cacgccgccg gttaaaccaa tcaggcgcat cggtgccaga gaagagacca gcgaccaggt
    26821 aatcgaagca aaaccgttgc cgaagaatgc cagcgccatc aggcacataa tcatcatcgg
    26881 atcgttggtg tagttagcgc ccataatgca ggtggagatc agcaagccgc agataatcgg
    26941 cgttttacgc gcaaagccca gtgaaaagcc cttacgtacc agcagatccg cgacccagcc
    27001 agagagcagg acgccgacaa acgccgcgag gaatggcacc gtggtcataa agcctgcttt
    27061 cagcgccgtg attccttttt cctgggttaa atagttcggg aaccaggtta agaaaaacca
    27121 cagtgtagaa gccaccgcaa attgcccaag ataaacgccg atcagtttac gatggaacac
    27181 cagtttccag tctttggctg ttaacggctg acgcgcctct ttcttcaccg gcgcatcacc
    27241 atccaccaga ccgccgccat cacgaatgta atccagttca gctttgctga tacctttggt
    27301 caggcgcggc ggctgataaa ccttaaacca aatcagcgac cagataatgc cgataccacc
    27361 agtgacaatg aacacccagt gccagctcaa catctcctga atccagatca gcagcggagt
    27421 caaaaacgcc agaccgacaa actgaccaga cgtataaaaa ccaacggcgg aagcgcgttc
    27481 atgttccggg aaccagctgg tcaccatccg gttattggtc gggaaagcgg gcgcttcgaa
    27541 aataccggtt atcgcacgca gaccaattaa tgacattaat cccgtggcaa agccctggaa
    27601 taaagtcgcc actgaccaac caaatatcgc aataaaataa gtcacgcgat ctaaaaacca
    27661 accgccgggg atctggcata gcgtataaag ccaggcgaag gccgaaaata catagcccat
    27721 ttgcgctttg gtaataccga actcttcctg aatatgggcg gaagccacgg ccaggttagc
    27781 gcggtcaaca taacaaatga ccaccgtaat aaagatcatc accagcgtca gataacgccg
    27841 acgccccggt tttgctgcat taacgggaat atccatagcg agcttcctcc agattttggg
    27901 catagcgaag ccgctcacca tgccctgtaa tttacagagg gttatatttt tgtattgctg
    27961 ttttagtgcc cgatgagggg cttacgtggc aggaattacc actctgctac gctgttatct
    28021 tcatgacgcc agagcggatt acgccagtcc ggggcatttt tactgaactc aataacttta
    28081 gcttcgtcga tttccacgcc taagcccggt ttcgttagcg gtttaaagaa gccgccaacc
    28141 atgctgaagt cttctttgtt tttcacaaag tcgagtaact ccgcgccttt gttgtaatga
    28201 atgcccatac tttgttcctg aagtacggcg ttataggaaa caaagtcgat atgcaggcaa
    28261 gccgccagtg caatcggtcc gagcggacag tgcggcgcaa gggtcacatc ataggcttct
    28321 gccattccgg caattttgta gcattcggta ataccgcctg catgggagag gtccggttgc
    28381 agaatcgaaa taccacctgc ctccagcacg cgtttaaaat cgaaacgtga gaacatgcgc
    28441 tcacccgccg ccagtggaat atgcgtttgt gccgccagtt tcgggtagta ttcggcttgt
    28501 tccgccagca ccggctcttc aataaacagc gggcgatacg gctccagttc tttaatcagc
    28561 actttcgcca ttggcgcgct gacgcgaccg tggaaatcaa gaccaaactc aatctgattg
    28621 ccaaaagctt cacgaatttg tgccacggtg ttgactgccg catctaccgc gcgggagtta
    28681 tcaattagcc ccagttcttc acaaccgttc agtttaaagg tatcgaagcc gatttcgcgc
    28741 agggttttaa tgccgtcgat aacatccgcc ggacgatcgc cgccgaccca actgtaggct
    28801 ttaattttgt cgcgaaccag gccgcccatc agttgccaga ccggcgcatt cagcacttta
    28861 cctttgatat cccataacgc ctggtcgatc ccggcgatgg cgctcatcag gattggaccg
    28921 ccacgataaa atccggcgcg atacatcact tgccataagt cattgatgcg cgatggatcc
    28981 tgaccaatca aatagtcacc cagctcgtga actgccgctt ccaccgtgcg ggcgcggcct
    29041 tcaatcacgg gctcgcccca accgaccacg ccttcatcgg tttcaatttt caggaacatc
    29101 cagcggggag gtaaacgata cgtggtaatt ttggtgattt tcattgcact gcctctcgat
    29161 acgccttaac aaatgctgct gcctgctgcg cggtgcgttc tacggactgc ccggcgcgat
    29221 agagatcgct gcctaagccc gccccagcac aacctgcgtc tatccactgc gccaggtttt
    29281 ctggcgtcac gccgccaacg gcaaagactg cgatgtccga tggcaatacc gcttttaacg
    29341 ctttgatgta ttgcggacca aaagccgatg acggaaatat tttcagcgcc tgcgcgcccg
    29401 cttcgagcgc ggtaaaggct tcggtcgccg tcgcgcagcc ggggcagacg gtcatgccgt
    29461 agcccaccgc acggcggatc acttcactat ggatattggg cgtaacgatg agctgacagc
    29521 ccatcctggc gagcgcatcg acctgttcag gtttcagtac cgtacctgcg ccaatcaacg
    29581 ccttgtcgcc atacgcatca acgatggcgg gaatgctttg ctcccattgt ggggaattca
    29641 gcgggatttc aaccgcgtcg aacccggcgt caatcaccgc gccaacatgc gccagcgcct
    29701 cgtcgggctt aatgccgcgc aaaatggcga tcagcggaag tttagtttgc cactgcatga
    29761 gcgatgctcc ttataccagc ctgaaatgcc gtgtcgcccg ccaccgccgt cacgtcgcaa
    29821 cccatcgcct gaaaggcttg ctggtagcgc gcggtcagcg atgttccggc gacaagggtg
    29881 atggcgtgtt gatgggtcac atagtcgcgc atactggcaa cctctgcgcc aatcaacaaa
    29941 ccagagagaa attcgctgac ctgttcgcgg ggaagtgttc ccagcacatg cgaggcgcga
    30001 acttcaaaaa gctgcggcaa tatggcgggc gcattaaggc cgcgctcaag gccagccgcg
    30061 aaggcatcgg cagagttttc ctgcggcggc aaacctgcgc caatcaatga gtgatttaac
    30121 agtaaatgat gtaattcacc ggtcatcacg gtgcgaaaat cgttgatttg ctggctatcg
    30181 gcctgcaccc atttgcaatg cgtaccgggc ataacataaa gagaggaagg agccagagct
    30241 cgcgcgccga tcaattgtgt ttcttcgccg cgcatcacat tgtggttatc gtcatgagag
    30301 acgcataatc cgggaataat ccagatattg tcgccaacag acgttaattg ttcgccaata
    30361 gacgaaaaac gggcaggaac agataaatac ggagcaactt tccagccgac attgctgccg
    30421 accattcctg ccatcaccac tggcgtattt tcttcacgcc agtcggtcgt gacttctgct
    30481 aacaccgcag ccggagattt tccgttcagg cgcgtgacgc ctgcttctga ttgcctgctc
    30541 tccaggcagt ggtcgccctg ataaagccag gcgcgcagat tggtcgatcc ccagtcaatt
    30601 gcgatgtagc gagctgtcat gtgatttcct ttaaccttcg tgtcgagctg gcgatcatgg
    30661 taagcgccgc ctgctctgcc gcatcgccgt cctgatgccg tattgcatcg aacagcgcct
    30721 tatgttcctg gagcgtttgc ggcatgttgg cctcatcgcc catccaggtt cgttcaaaaa
    30781 ccgcccgctg cagcgaactg atcgcaatgc taagttgctg taacaccggg ttatgcaccg
    30841 actgcagcac cgcctcgtgg tagcgaatat ccgcttcgtt aaacgcttcg cggtcctgat
    30901 tgttggcaat catctcgttc agcgccgatt caatctgcgc cagatcgctg gaagtcgcgc
    30961 gctctgctgc ccaacgggca atcgccggtt ccaccagatt tcgcacttcg ctcatggcac
    31021 tgataagccg tgggtcgtag tcgttttcca acacccattg cagtacgtca gtgtcgaggt
    31081 aattccactg gttacgcggt gccacaaacg ccccgcgata acgtttcatt tcaatcagcc
    31141 gcttcgccat cagcgaacgg aacacctcac ggatgatgtt gcgcgaggtt gcaaactctt
    31201 cacagagctc cgcctcagcc ggaagtggcg agccaggtac gtatttgccg tgaacgatct
    31261 gtttacccag cgtaatgaca atgcgatcgg ttttattgag agtcatggag agtccttgtg
    31321 ctctgtatgt tcttctctac tttaccccga tcgatgcata acgcggcaac tttgtagtac
    31381 cagcgtgatg acgttcgcgt ttgccgtgtg agtaatgtag tacaaactta tattgttgta
    31441 ctacaattta gatcacaaaa agaacaatgc ataaaaaaat gacatgcgtc gggcagaaat
    31501 ctgaaaaggg atatcaggcg ctaaacagga gggaaagaag agtatgcttt caacggctta
    31561 gctactcgtt taaaggatta atcatgaagt tgaattttaa gggatttttt aaggctgccg
    31621 gtttattccc actggcgctg atgctttcag gctgtatctc gtatgctctg gtttcccata
    31681 ccgcaaaggg tagttcagga aagtatcaat cgcagtcaga caccatcact gggctatcgc
    31741 aggcaaaaga tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc
    31801 ttatcactga tggtgccgat gacatcgtta agatgctcaa tgatccagca cttaaccggc
    31861 acaatattca ggttgccgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta
    31921 ccggcacaat atcgctttac tactactgga ataacgaaga agaaaaggca ctggcaacgc
    31981 attatggttt tgcctgtggt gttcaacact gtaccaggtc actggaaaac ctaaaaggca
    32041 caatccatga gaaaaataaa aacatggatt actcaaaggt gatggcgttc taccatccat
    32101 ttaaagtgcg attttatgaa tactattcac ccagaggcat tccggatggt gtttccgcag
    32161 cattactgcc agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg
    32221 tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgtcc
    32281 actgattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttggat
    32341 ttggtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat cgccacaccg
    32401 acgcctgcat attcaatcat tgcgatatcg ttttcctgat cgccaatcgc catgatttct
    32461 tccggtttaa tacctaacac gtcggccagt gatttcaccc ccgtaccttt gttaacgcgt
    32521 ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact
    32581 tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact
    32641 ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag cggaatggtg
    32701 gcaacgaagg attcatgcac cgtgtagtag ctgatatcac ggttggcggt gtacagcgtg
    32761 gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga
    32821 tagtcgtcat agctgagagc agtttgcgcc acggtgctac catcagcggc cttctgtacc
    32881 agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg
    32941 tagttgtgca cacctgcata cgggcgaccc gtcgttagca cgacattcac gccacgggcg
    33001 cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg
    33061 gtgccatcca tatcgatagc aatgagtttg atagccatga gttccccagg tagattagtt
    33121 cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg
    33181 gatgcaacgc gcgttgcgtg ctccccattt gcttagcgga agagtttccc tttcagcagt
    33241 tccatgcctg cggaaagcag atcgttattg gcttgtggag acacttcacc ttgcggtgag
    33301 agcgcatcaa taatcttcgg caattgttct gccagtaaac tggaagctgt actggtatcc
    33361 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ttccagttgc
    33421 tcgccactaa ccgattgatt gccctgttga ttactcagcc aggttgagag aatggcccct
    33481 aagccgccac tttgcagttt ttccagcagc acctgaatgc cgccctgctc ctcaacccaa
    33541 cttaaaatcg cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    33601 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatctg
    33661 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    33721 tatagcttct tgccagatgc ggcgctaacg ccttacccgg cctacaaaat catgaaaatt
    33781 caatacattg caagattctc gtaggcctga taagcgtagc gcatcaggca cgctcgcatg
    33841 gttaacgcca ttaaatatcg atattcgccg ctttcagggc gttctcttca ataaacgcac
    33901 ggcgcggttc aacggcgtcg cccatcagcg tggtgaacag ctggtcagca gcaatcgcat
    33961 ccttaacggt aacgcgcagc atacgacgac tttccgggtc catggtggtt tcccacagct
    34021 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggccg cgacgggact
    34081 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    34141 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    34201 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    34261 gggtacgcac gcgaacaatc ggctcgaaca ggttttgctc ggcattggtg tggacatcaa
    34321 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    34381 agcgggtaac ggtctgctca tcagagaggt cggcttccgt cagcgtcggc tgatagataa
    34441 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    34501 cgttgtactc agacaccagt ttctctaacg cttcgccagc caatgccggt gcactggcgt
    34561 tggtgtgcag cgttgcgcca tccagcgcga tggagatttg gtactgatcc atcgcttcgt
    34621 cgtctttaat atactgttcc tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    34681 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaagaag gtcaacagca
    34741 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    34801 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    34861 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcaacg ttgaggattt
    34921 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    34981 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    35041 ctgccagttt gcccggcagg cccgctaagt cgagcgcacc tttacggcgg gtcatttcac
    35101 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    35161 tcgcgtcggt tgggttttcc agcaggtatt ctgccagcag ttcgttcatc tgctgttcaa
    35221 ccgccgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    35281 ccggcacttt cacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    35341 tgactttggc ttttttgctg tagccttctt tgtccatgta ggcattcagg gtacgggtca
    35401 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    35461 agcagtagat gttttcctgg aagccatcgt tccactgcaa cgccacttcg acgccaatac
    35521 cgtctttttc ggtggagaag tagaagatat tcgggtggat cggcgttttg ttcttgttca
    35581 gatattcaac gaacgccttg atgccacctt catagtggaa gtggtcttct ttgccgtcgc
    35641 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    35701 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    35761 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccagcggg gcctgcggta
    35821 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctggataacc agctccagtt
    35881 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    35941 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    36001 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    36061 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    36121 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    36181 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    36241 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatcc
    36301 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    36361 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    36421 agcaacccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    36481 cgccgcccgc tttcacgggt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    36541 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    36601 aagtccgctt tgtgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    36661 cgttccagta cctcagcata ttctgtctct ttctcccagc cgcgctggaa agagaaagtc
    36721 agagaaaact cagggagaaa ttgcttacag gtatcagcca tatcggccgc gataccggcg
    36781 ctgtactggg cgcgccaggt gctgatttgc tccgccagcg ggatcagttc tttatcccac
    36841 gggcgtagct gttcgtaacg tgtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    36901 cgcttgagat tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca tccccagtcg
    36961 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    37021 agctgcattg gcatcaggtg cgccagttcc gcgactttat gcccgtcagt accgtcgatg
    37081 cggactttgc tgtcgccctg tttgtccttg gttaagccaa tcgccgtctc gcgctcttcg
    37141 ccctgtaatc gcccatggag aacaaatgcc tcctgctcat ggcgaatgac gcgaccaatc
    37201 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    37261 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    37321 gtttcaatgt tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    37381 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    37441 aatcggtcag catcatgcgg acgttttcgc atttcagcgc gttcagaaca tccagcacat
    37501 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    37561 ccgcttcttc ctgttccggg ttgttggcgg tgattttcag ctggttttcg ctgacataca
    37621 ggcgcacgcc gcggaatttc tcgttagaga gaattgccgc acgggcaaac gcctgcttga
    37681 gcagatcgca gccagcttcc agatgtttgt ccggattctt cggcagaacg cggcggtaat
    37741 ccgggaagcg accatccacc agtttggagg tgaagataaa gtcgccaacg tgggcgcgaa
    37801 tattgttgct gccaatctgc acgcgcagcg gattgtcgcc gccgtcgagc atacgcatca
    37861 gttcaatcac gcctttacgc ggtacgatca ccgaatggct tggcaaagat tgaccaattg
    37921 gcattgaaca gaccgccaga cggtggccgt cggttgccac ggtgcgcagt tcttcacctt
    37981 cggtttcaaa cagcatacca tttaagtaat agcgaacgtc ctgatgcgcc atcgaaaact
    38041 gggtcgcttc aatcagacgc ttcatcgttg cctgcggcag ggtaaattcg acttcactct
    38101 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    38161 gcccggagcg taccagcatc cgttcacctt ccagctgcac ggcaatttcc gcgccttcag
    38221 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    38281 gtggctgaac cagcgcaaca cgtgccacca tttccatctc gagatcagta ccggtcagcg
    38341 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    38401 cacctaacgg accgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    38461 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    38521 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    38581 cgaccaccaa acgcatcgcc aatctccggc agactgtggt tagtcagctc tttcgccagc
    38641 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    38701 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    38761 ttttcctgca atgccagcaa gtcgcgcagc gcctcacgca cgaagtcgat ggtgatcgcc
    38821 cgtccggtaa agttggcatt ggcaatgacg cggttcagcg ccccttccag ctcacgtacg
    38881 ttagatcgta gacgcttggc gataaagaac gccacttcgc ccggcaaacg aatgtcgttt
    38941 tcgtcggcct ttttcatcag gatcgccaca cgggtttcca gctctggcgg ttcgatcgcc
    39001 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    39061 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggta
    39121 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    39181 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    39241 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    39301 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    39361 ccataaagga acaacgggtt ataggcaccg ccagggttat ccgccacctg gcgagccgcc
    39421 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    39481 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    39541 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    39601 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    39661 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    39721 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    39781 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    39841 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    39901 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    39961 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    40021 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    40081 cgatttatcc acaggacttt ccagaactcg ctaagtgtaa acgatcctgc cgcgaggcgg
    40141 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    40201 atctaataaa gatccaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    40261 ccggcgcgcc atgctggttt ccactggtgt gaggtcgtac attttccctg cgaaaaggtg
    40321 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg agtgtacaat tattacaatc
    40381 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    40441 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    40501 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    40561 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    40621 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    40681 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    40741 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    40801 aacgttcgac gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga aagcttccgt
    40861 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    40921 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    40981 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    41041 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    41101 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    41161 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    41221 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    41281 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    41341 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    41401 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    41461 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgct
    41521 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    41581 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    41641 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    41701 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    41761 gcagaacgct ggcgagaaac cgctggaaat ctccaccttt ggtcagttga agcaatccat
    41821 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgcgg
    41881 cgcggcgtac tccacgcctg acgagaagta cgagaaatac aagttcgata ccattgccga
    41941 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcgatgctgc aacagtattt
    42001 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    42061 taacggcatc gccgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    42121 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    42181 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    42241 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    42301 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    42361 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    42421 caaacagcgt atcagccagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    42481 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    42541 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    42601 acaggacccg tactacatct tgccgatcct gatgggcgta acgatgttct tcattcagaa
    42661 gatgtcgccg accacagtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    42721 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact atatcgtcag
    42781 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    42841 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    42901 agttgaccgc cttttttctt ttcgtagggc ggataagcac cgcgcatccg ccacacaaag
    42961 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    43021 tggcggcgtt ggcatcctgc gcatctccgg cttcaaagcc cgtgaagttg ccgaaaccgt
    43081 gctgggtaaa ctacctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    43141 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    43201 agatgtgctg gaactgcaag gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    43261 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    43321 tcttaacgat aaactcgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    43381 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    43441 caaccatctg gtggaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    43501 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    43561 gattgccgaa ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    43621 gatgaaggtg gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgt taaacgcgct
    43681 ggcgggccgt gaagcagcaa tcgtaactga tatcgccgga accacacgtg acgtgctgcg
    43741 tgagcatatc cacattgacg gaatgccgct gcatatcatc gataccgctg ggctacgtga
    43801 agccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    43861 cgaccgcgtg ctgtttatgg tcgatggcac cacaacagac gccgtggatc cggcagagat
    43921 ctggccggaa tttattgccc gtctgccagc gaaactgccg atcaccgtgg tgcgcaataa
    43981 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    44041 tctctcggca aggactggtg aaggcgtgga cgtgctgcgt aaccatctca aacagagcat
    44101 gggctttgac accaacatgg aaggcggctt cctggcgcgt cgtcgccacc tacaggcgct
    44161 ggaacaggca gcggaacatc tacaacaggg caaagcgcaa ctgttgggag cctgggcagg
    44221 tgaactgctg gcggaagagt tgcgtctggc acagcagaac ttaagcgaaa tcaccgggga
    44281 atttacttca gacgacctgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    44341 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    44401 gttattcctc aacccttttt ttaaacatta aaattcttac gtaatttata atcttaaaaa
    44461 aaagcattta atattgctcc ccgaacgatt gtgattcgat tcacatttaa acaattttag
    44521 aatagacaaa attcttgagt attatactgt agcctcgtgt ctttcgagga taagtgcatt
    44581 atgaatatct tacatatatg tgtgacctca aaatggttca atatcgacaa taagattgtc
    44641 gatcaccgcc cttaatttac ccttctgtag ccatcaccag agccaaaccg attagattca
    44701 atgtgaacga ttagtttgct atatcttaat tttgcctttt gcaaaggcca tctctcgttt
    44761 atttacttat tttagtaaat gatggtgctt gtatatatac atggcgaatt aatggatatt
    44821 gcagatgtaa tattcacagg gatcactgta attaaaataa atgaaggatt atgtaatgga
    44881 aaactttaaa catctccctg aaccgttccg cattcgtgtt attgagccag taaaacgtac
    44941 tactcgcgct tatcgtgaag aagcaattat taaatccggt atgaacccgt tcctgctgga
    45001 tagcgaagat gtgtttatcg atttactgac cgacagcggc accggggcgg taacccaaag
    45061 tatgcaggca gcgatgatgc gcggcgacga agcctacagc ggcagccgca gctactatgc
    45121 gttagccgag tcagtgaaaa atatctttgg ttatcaatac accattccga ctcaccaggg
    45181 ccgtggcgca gagcaaattt atattccggt actgattaaa aaacgcgagc aggaaaaagg
    45241 cctggatcgc agcaaaatgg tggcattctc taactatttc tttgatacca cgcagggcca
    45301 tagccagatt aacggctgta ccgtgcgtaa cgtctatatc aaagaagcct tcgatactgg
    45361 cgtgcgttac gactttaaag gtaactttga ccttgaggga ttagaacgcg gtattgaaga
    45421 agttggcccg aataacgtgc cgtatatcgt tgcaaccatc accagtaact ctgcaggtgg
    45481 tcagccggtt tcactggcaa acttaaaagc gatgtacagc atcgcgaaga aatacgatat
    45541 tccggtggta atggactccg cacgctttgc tgaaaacgcc tatttcatca agcagcgtga
    45601 agcagaatac aaagactgga ccatcgagca gatcacccgc gaaacctaca aatatgccga
    45661 tatgctggcg atgtccgcca agaaagatgc gatggtaccg atgggcggct tgctgtgcat
    45721 gaaagacgac agcttctttg atgtgtacac cgagtgcaga accctttgcg tggtgcagga
    45781 agggttcccg acatatggcg gcctggaagg cggcgcgatg gagcgtctgg cggtaggtct
    45841 gtatgacggc atgaatctcg actggctggc ttatcgtatc gcgcaggtac agtatctggt
    45901 cgatggtctg gaagagattg gcgttgtctg ccagcaggcg ggcggtcacg cggcattcgt
    45961 tgatgccggt aaactgctgc cgcatatccc ggcagaccag ttcccggcac aggcgctggc
    46021 gtgcgagctg tataaagtcg ccggtatccg tgcggtagaa attggctctt tcctgttagg
    46081 ccgcgatccg aaaaccggta aacaactgcc atgcccggct gaactgctgc gtttaaccat
    46141 tccgcgcgca acatatactc aaacacatat ggacttcatt attgaagcct ttaaacatgt
    46201 gaaagagaac gcggcgaata ttaaaggatt aacctttacc tacgaaccaa aagtattgcg
    46261 tcacttcacc gcaaaactga aagaagttta attaatacta cagagtggct ataaggatgt
    46321 tagccactct cttaccctac atcctcaata acaaaaatag ccttcctcta aaggtggcat
    46381 catgactgat caagctgaaa aaaagcactc tgcattttgg ggtgttatgg ttatagcagg
    46441 tacagtaatt ggtggaggta tgtttgcttt acctgttgat cttgccggtg cctggttttt
    46501 ctggggtgcc tttatcctta tcattgcctg gttttcaatg cttcattccg ggttattgtt
    46561 attagaagca aatttaaatt atcctgtcgg ctccagtttt aacaccatca ccaaagattt
    46621 aatcggtaac acctggaaca ttatcagcgg tattaccgtt gccttcgttc tctatatcct
    46681 cacttatgcc tatatctctg ctaatggtgc gatcattagt gaaacgatat caatgaattt
    46741 gggctatcac gctaatccac gtattgtcgg gatctgcaca gccattttcg ttgccagcgt
    46801 attgtggata agttcgttag ccgccagtcg cattacctca ttgttcctcg ggctgaagat
    46861 tatctccttt gtgatcgtgt ttggttcttt cttcttccag gtcgattact ccatcctgcg
    46921 cgatgccacc agcaccactg cgggaacgtc ttacttcccg tatatcttta tggctttgcc
    46981 ggtgtgtctg gcgtcatttg gtttccacgg caatattccc agcctgatta tttgctatgg
    47041 aaaacgcaaa gataagttaa tcaaaagcgt ggtatttggt tcgctgctgg cgctggtgat
    47101 ttatctcttc tggctctatt gcacgatggg gaatattccg cgcgaaagct ttaaggcgat
    47161 aatctcctca ggcggcaacg ttgattcgct ggtgaaatcg ttcctcggca ccaaacagca
    47221 cggcattatc gagttttgcc tgctggtgtt ctctaactta gctgttgcca gttcgttctt
    47281 tggtgtcacg ctggggttgt tcgattatct ggcggacctg tttaagattg ataactccca
    47341 cggcgggcgt ttcaaaaccg tgctgttaac cttcctgcca cctgcgttgt tgtatctgat
    47401 cttcccgaac ggctttattt acgggatcgg cggtgccggg ctgtgcgcca ccatctgggc
    47461 ggtcattatt cccgcagtgc ttgcaatcaa agctcgcaag aagtttccca atcagatgtt
    47521 cacggtctgg ggcggcaatc ttattccggc gattgtcatt ctctttggta taaccgtgat
    47581 tttgtgctgg ttcggcaacg tctttaacgt gttacctaaa tttggctaaa tccttcaaga
    47641 agccagccat tcgctggctt cttgcctctc aggaaatcac ttatgtccaa atggcaactc
    47701 gcctgattct ccttcaccac gtatgctttg cgtcacctta ctattaggac gctttagccc
    47761 atgtcccgct ttttaatttg tagttttgcc ctggttttac tttatcccgc cgggattgat
    47821 atgtacctcg ttggtttacc gcgcatcgcc gacgatctca atgccagcga agcgcagttg
    47881 catattgcgt tctccgttta tctggcgggc atggcagctg cgatgttatt tgccggtaaa
    47941 gtggccgatc gttcagggag aaagcctgtc gccatccccg gcgcggcgct atttattatc
    48001 gcttcggtgt tctgttcact ggctgaaacc agcacgttat ttcttgcagg ccgatttcta
    48061 caggggttgg gcgcaggctg ttgttacgta gtggcgttcg ccattttgcg cgacacgctg
    48121 gatgatcgac gtcgggctaa agtgctgtca ttactcaacg gtattacctg catcattccg
    48181 gtgttagcgc cagtgctcgg acatctgatt atgcttaaat tcccgtggca gagtctgttc
    48241 tgggcgatgg caatgatggg catcgcggta ctgatgttgt ctttgtttat tttaaaagaa
    48301 acgcgcccag cgtcccccgc tgcttcggac aaaccacgag aaaatagcga gtcgctgctt
    48361 aatcggtttt tcctcagccg tgttgttatc accaccctca gcgtttcggt gatcctcact
    48421 ttcgtcaata catcgccggt attgctgatg gaaatcatgg gttttgagcg cggagaatac
    48481 gccaccatta tggcgttgac tgctggcgtc agcatgaccg tttcattctc cacgccattt
    48541 gcgctgggaa tttttaagcc acgtacgttg atgatcacct cgcaggtgtt attccttgca
    48601 gcggggatca cccttgccgt ttcaccttcc catgcggttt ctctgtttgg tatcacgctg
    48661 atttgcgccg gtttctcggt aggttttggc gtagcgatga gtcaggcgtt aggaccattt
    48721 tcattacgcg cgggcgtagc cagctcgacc ttaggtattg cgcaggtttg cggttcgtca
    48781 ctgtggattt ggctggcagc ggtggttggt atcggcgcat ggaatatgct gatcgggatt
    48841 ctgattgcct gtagcatagt gagcctgttg ctgattatgt tcgtcgcgcc tggacgcccc
    48901 gttgccgctc atgaagaaat ccatcaccac gcttgatctc aatctgctgc tctgtctgca
    48961 actgctgatg caggagcgca gcgtgaccaa agcggcgaag cggatgaacg tgaccccttc
    49021 agcggtgagt aagtcgctgg caaagttaag agcgtggttt gacgacccgc tctttgtgaa
    49081 ctcaccgctg ggcctgtcgc ccacaccgct aatggtcagc atggagcaaa atctggcgga
    49141 atggatgcaa atgagcaatc tgctgctgga taaaccgcac caccagacac cgcgcggcct
    49201 gaagtttgag ttggcggcgg aatcgccgct gatgatgatc atgcttaatg cgctgtcgaa
    49261 acggatctac caacgttacc cgcaggcgac catcaaacta cgtaactggg attacgattc
    49321 cttagatgcc attactcgtg gtgaagtgga tattggtttt tccggtcgcg aaagccatcc
    49381 tcgctcgcgg gagctgttaa gctcgctacc gttagccatt gattatgaag tgctgtttag
    49441 tgatgtaccc tgcgtctggt tacgcaaaga tcatccggca ctgcatgaaa cgtggaatct
    49501 ggacaccttt ttacgttatc cgcatatcag catttgctgg gaacagagcg acacctgggc
    49561 gctggacaat gtgttacagg agctggggcg cgaacgcacg attgctatga gcctgccgga
    49621 atttgagcag tcactgttta tggcagcgca acccgacaat ctgctactgg cgaccgcgcc
    49681 gcgctactgt cagtactaca atcaactcca tcaactgccg ttggttgctc ttcctctccc
    49741 gtttgacgaa agccagcaaa aaaagctgga agttcctttt accctgctgt ggcataaacg
    49801 gaacagccat aatccgaaga tcgtctggtt acgggaaacc attaaaaacc tttacgcgtc
    49861 gatggcataa ccgaatcgta tgaaacgcga cccaatttca caataa
//
LOCUS       DAZFXT010000019        49286 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.019, whole genome shotgun sequence.
ACCESSION   DAZFXT010000019 DAZFXT010000000
VERSION     DAZFXT010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49286)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49286)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49286
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.019"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            165..1925
                     /gene="tssF"
                     /locus_tag="ACHKW0_001242"
     CDS             165..1925
                     /gene="tssF"
                     /locus_tag="ACHKW0_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7474109.1"
                     /translation="MDDLTQRYYEAEMRYLREAGKEFAQAYPDRAAMLNLDKPGARDP
                     YVERLFEGFAFLMGSLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSVVELSTDHRQM
                     KQSETLSDFQVLSRPVGERRTRCFYSATRDITLHPLALPDVSLQYEPDGRSVIRLRFE
                     CGPLVGDWSQIDLSRLPFYLNADSPVACALHRALTLGTQQFWLRLPGQDRRTLDAHFS
                     PLGFEDNDRLWPKGESAFSGYQLLLEYFTFREKFMFVALNGLELVAWPEGITGFEIDV
                     VLNENWPHDLPFDSDNIRLHCVPVINLFPPEADPLHLSPLENEFLLRPMRIQDGHTEI
                     YSVDNIISSRHTGSQAYVPFSSFRHRGGMLRHDAPERYYHTRVKRGPSGLHDTWLILG
                     GDAFDADRMLEDETLSLSLTGTNGHLPRKALQSTLLDRPVHASQNVLRVRNLCAPTQP
                     CYPPARDRFHWRVLSHLGSNFLSMMDNAEILRGTLALYDWTESEMNRRRLEAIVDVQH
                     SLIQRFERGFLLRGVDIQVTLDSNGFAGEGDITLFGELLHRFFALYADIHLFTQLTLI
                     LQPTGKCLQWTEHHSQRVPG"
     gene            1889..2968
                     /gene="tssG"
                     /locus_tag="ACHKW0_001243"
     CDS             1889..2968
                     /gene="tssG"
                     /locus_tag="ACHKW0_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7474110.1"
                     /translation="MDRASQPARTGLTDRLAPDIARINFYRFCQFLEQSQQNAPLGST
                     DNPATDAVRFRPHPGMGFPVSELKNVERDAGNPNVPPTVRTTFLGLYGVDSPLPTAYL
                     DYITQRHDGHDAVMAFLDIFNHRFITQYYRIWRKYNYPASFEAGAMDDISRCLLGLIG
                     LGIPGSENHIATPVSRFLALLSVMRLPTRTAEGVTALVGLLAPLTKATVVPHDPQPVI
                     LPAPAGLSKNSRISLKTRTLLGRTGTDVNSQLLLKLYTEDAAEARGWLPGGQLHSDLL
                     VLLRVYLGWRCQARLQLTLPVSLLPAARLGKQRVQISRTGILRASFAAPATGTVTVSL
                     GRYQGLIPALSIRNRESMTHVSYSF"
     gene            2949..3485
                     /gene="tssJ"
                     /locus_tag="ACHKW0_001244"
     CDS             2949..3485
                     /gene="tssJ"
                     /locus_tag="ACHKW0_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7474111.1"
                     /translation="MFPIRFKRPALLCMAMLTVVLSGCGLIQKVVDESKSVASAVFYK
                     QIKILHLDFFSRSALNTDAEDTPLSTMVHVWQLKTREDFDKADYDTLFMQEEKTLEKD
                     VLAKHTVWVKPEGTASLNVPLDKETQFVAIIGQFYHPDEKSDSWRLVIKRDELEADKP
                     RSIELMRSDLRLLPLKDK"
     gene            3489..3917
                     /gene="tssE"
                     /locus_tag="ACHKW0_001245"
     CDS             3489..3917
                     /gene="tssE"
                     /locus_tag="ACHKW0_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000106970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7474112.1"
                     /translation="MSTPSLYEMLTFSFSGELPLEQVSERDQLILSVMDNMQRIINCR
                     AGTLAHLPDYGLPDLSLIHQGMVAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPG
                     YLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY"
     gene            3917..5293
                     /locus_tag="ACHKW0_001246"
     CDS             3917..5293
                     /locus_tag="ACHKW0_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
                     /protein_id="HFO7474113.1"
                     /translation="MASNANFISQFVMGGDPCTYKESGELQAEMSKLTHPARPDVDWR
                     RVEKLSLALFRQNGVELQTQVCYVLAITRRQGLAGMADGLGSLDILLQRWADFWPVQV
                     HSRISLLSWVTEKMQQALRTLDIQYQDLPQIYRCVQHLSAIETTLQQCELWHMTKLDV
                     LAGQFRNTALRLERLAPQGAETTITPPELPRREMKQPEKSEESPQPVFATRPAQQNDK
                     DASPSAPSPEISWQRTWPIFMAGMVVMACLGGTGLWGWSQLNQPDALIQRIQLSVMPL
                     PQSLESGELAKLDVKDKALLAQDRTIAASQMQLEQLNKLPARWPLEQGYRQLRQLDAL
                     WPDNPQVRALNAQWRKQRELSALSTEALNGYAQAQSQLQRLSAQLDALDERKGRYLTG
                     SELKTAVYGIRQSLKEPPLEELLRQLEEQKQTGEVSPTLLTQIDTRLNQLLNRYVILL
                     DTKVEQSQ"
     gene            5358..5906
                     /locus_tag="ACHKW0_001247"
     CDS             5358..5906
                     /locus_tag="ACHKW0_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HFO7474114.1"
                     /translation="MYKKERNRPFETREEVLAYHKREKIPCLECGKLLIFLPRHIWFV
                     HGIRADEYRVKWNIPKHIALAGISYLEKQSEYMKARIAKGKLDPAEQIAMMRIKGRQL
                     KSDPDWRNRSGSILKKQYARRDILKNRVWERSPSIKSVSVELKTEAIRRMKNRKVVGE
                     KVQDIADDLNVSISRLYHWLKM"
     gene            complement(6252..7505)
                     /gene="amiC"
                     /locus_tag="ACHKW0_001248"
     CDS             complement(6252..7505)
                     /gene="amiC"
                     /locus_tag="ACHKW0_001248"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Like AmiA, dependent on TAT (Twin-Arginine
                     Translocation) system for export; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HFO7474115.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            7737..9068
                     /gene="argA"
                     /locus_tag="ACHKW0_001249"
     CDS             7737..9068
                     /gene="argA"
                     /locus_tag="ACHKW0_001249"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004042 - acetyl-CoA:L-glutamate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HFO7474116.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(9130..10956)
                     /gene="recD"
                     /locus_tag="ACHKW0_001250"
     CDS             complement(9130..10956)
                     /gene="recD"
                     /locus_tag="ACHKW0_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HFO7474117.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(10956..14498)
                     /gene="recB"
                     /locus_tag="ACHKW0_001251"
     CDS             complement(10956..14498)
                     /gene="recB"
                     /locus_tag="ACHKW0_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HFO7474118.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIATVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSVGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEAERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDKQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLNQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYERHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIDLMDEMFAGMTLEEA"
     gene            complement(14491..17379)
                     /gene="ptrA"
                     /locus_tag="ACHKW0_001252"
     CDS             complement(14491..17379)
                     /gene="ptrA"
                     /locus_tag="ACHKW0_001252"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HFO7474119.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSDKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(17555..20923)
                     /gene="recC"
                     /locus_tag="ACHKW0_001253"
     CDS             complement(17555..20923)
                     /gene="recC"
                     /locus_tag="ACHKW0_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HFO7474120.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCHEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(20936..21259)
                     /gene="ppdC"
                     /locus_tag="ACHKW0_001254"
     CDS             complement(20936..21259)
                     /gene="ppdC"
                     /locus_tag="ACHKW0_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HFO7474121.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(21244..21651)
                     /gene="ygdB"
                     /locus_tag="ACHKW0_001255"
     CDS             complement(21244..21651)
                     /gene="ygdB"
                     /locus_tag="ACHKW0_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HFO7474122.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVINGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(21648..22211)
                     /gene="ppdB"
                     /locus_tag="ACHKW0_001256"
     CDS             complement(21648..22211)
                     /gene="ppdB"
                     /locus_tag="ACHKW0_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7474123.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(22202..22672)
                     /gene="ppdA"
                     /locus_tag="ACHKW0_001257"
     CDS             complement(22202..22672)
                     /gene="ppdA"
                     /locus_tag="ACHKW0_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HFO7474124.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(22857..23651)
                     /gene="thyA"
                     /locus_tag="ACHKW0_001258"
     CDS             complement(22857..23651)
                     /gene="thyA"
                     /locus_tag="ACHKW0_001258"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HFO7474125.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(23658..24533)
                     /gene="lgt"
                     /locus_tag="ACHKW0_001259"
     CDS             complement(23658..24533)
                     /gene="lgt"
                     /locus_tag="ACHKW0_001259"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HFO7474126.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(24684..26930)
                     /gene="ptsP"
                     /locus_tag="ACHKW0_001260"
     CDS             complement(24684..26930)
                     /gene="ptsP"
                     /locus_tag="ACHKW0_001260"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7474127.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(26943..27473)
                     /gene="rppH"
                     /locus_tag="ACHKW0_001261"
     CDS             complement(26943..27473)
                     /gene="rppH"
                     /locus_tag="ACHKW0_001261"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HFO7474128.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            complement(27826..27972)
                     /gene="ygdT"
                     /locus_tag="ACHKW0_001262"
     CDS             complement(27826..27972)
                     /gene="ygdT"
                     /locus_tag="ACHKW0_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgdT"
                     /protein_id="HFO7474129.1"
                     /translation="MLSTESWDNCEKPPLLFPFTALTCDETPVFSGSVLNLVAHSVDK
                     YGIG"
     gene            28158..28847
                     /gene="mutH"
                     /locus_tag="ACHKW0_001263"
     CDS             28158..28847
                     /gene="mutH"
                     /locus_tag="ACHKW0_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HFO7474130.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDIIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            28916..29629
                     /gene="ygdQ"
                     /locus_tag="ACHKW0_001264"
     CDS             28916..29629
                     /gene="ygdQ"
                     /locus_tag="ACHKW0_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HFO7474131.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            29767..29985
                     /gene="ygdR"
                     /locus_tag="ACHKW0_001265"
     CDS             29767..29985
                     /gene="ygdR"
                     /locus_tag="ACHKW0_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HFO7474132.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            30093..31133
                     /gene="tas"
                     /locus_tag="ACHKW0_001266"
     CDS             30093..31133
                     /gene="tas"
                     /locus_tag="ACHKW0_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HFO7474133.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(31165..32358)
                     /gene="lplT"
                     /locus_tag="ACHKW0_001267"
     CDS             complement(31165..32358)
                     /gene="lplT"
                     /locus_tag="ACHKW0_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HFO7474134.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(32351..34510)
                     /gene="aas"
                     /locus_tag="ACHKW0_001268"
     CDS             complement(32351..34510)
                     /gene="aas"
                     /locus_tag="ACHKW0_001268"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008779 -
                     acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008922 - long-chain fatty acid
                     [acyl-carrier-protein] ligase activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HFO7474135.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRMPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            35096..36127
                     /gene="galR"
                     /locus_tag="ACHKW0_001269"
     CDS             35096..36127
                     /gene="galR"
                     /locus_tag="ACHKW0_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HFO7474136.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(36134..37396)
                     /gene="lysA"
                     /locus_tag="ACHKW0_001270"
     CDS             complement(36134..37396)
                     /gene="lysA"
                     /locus_tag="ACHKW0_001270"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HFO7474137.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGLVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            37518..38453
                     /gene="lysR"
                     /locus_tag="ACHKW0_001271"
     CDS             37518..38453
                     /gene="lysR"
                     /locus_tag="ACHKW0_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HFO7474138.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(38440..39132)
                     /gene="ygeA"
                     /locus_tag="ACHKW0_001272"
     CDS             complement(38440..39132)
                     /gene="ygeA"
                     /locus_tag="ACHKW0_001272"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HFO7474139.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIDECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(39261..40679)
                     /gene="araE"
                     /locus_tag="ACHKW0_001273"
     CDS             complement(39261..40679)
                     /gene="araE"
                     /locus_tag="ACHKW0_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HFO7474140.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(40994..41755)
                     /gene="kduD"
                     /locus_tag="ACHKW0_001274"
     CDS             complement(40994..41755)
                     /gene="kduD"
                     /locus_tag="ACHKW0_001274"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HFO7474141.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(41785..42621)
                     /gene="kduI"
                     /locus_tag="ACHKW0_001275"
     CDS             complement(41785..42621)
                     /gene="kduI"
                     /locus_tag="ACHKW0_001275"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008697 -
                     4-deoxy-L-threo-5-hexosulose-uronate ketol-isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0045490 - pectin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HFO7474142.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(42908..44089)
                     /gene="yqeF"
                     /locus_tag="ACHKW0_001276"
     CDS             complement(42908..44089)
                     /gene="yqeF"
                     /locus_tag="ACHKW0_001276"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HFO7474143.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            44344..45573
                     /gene="yqeG"
                     /locus_tag="ACHKW0_001277"
     CDS             44344..45573
                     /gene="yqeG"
                     /locus_tag="ACHKW0_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7474144.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            46033..46665
                     /gene="yqeH"
                     /locus_tag="ACHKW0_001278"
     CDS             46033..46665
                     /gene="yqeH"
                     /locus_tag="ACHKW0_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7474145.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
     gene            46999..47808
                     /gene="yqeI"
                     /locus_tag="ACHKW0_001279"
     CDS             46999..47808
                     /gene="yqeI"
                     /locus_tag="ACHKW0_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7474146.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTHSLIATVPRRGFK
                     IHNDTNIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYREVNRD"
     gene            47801..48283
                     /gene="yqeJ"
                     /locus_tag="ACHKW0_001280"
     CDS             47801..48283
                     /gene="yqeJ"
                     /locus_tag="ACHKW0_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeJ"
                     /protein_id="HFO7474147.1"
                     /translation="MIDYKKNLLFILVFISGFILFTVYSYTAEKMIYNETCTANWVIF
                     NDQGRANLTIDFMFNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHLTS
                     KKVNKFEIMDQVDDDRLVQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHCAR
                     "
     gene            complement(48316..48396)
                     /locus_tag="ACHKW0_001281"
     CDS             complement(48316..48396)
                     /locus_tag="ACHKW0_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474148.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(48432..48856)
                     /gene="yqeK"
                     /locus_tag="ACHKW0_001282"
                     /pseudo
     CDS             complement(48432..48856)
                     /gene="yqeK"
                     /locus_tag="ACHKW0_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqeK"
     gene            49050..>49286
                     /locus_tag="ACHKW0_001283"
     CDS             49050..>49286
                     /locus_tag="ACHKW0_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474149.1"
                     /translation="MKPRNINNSLSLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLIHDLSIKIADISEEIIQQAILSAM"
BASE COUNT        12472 a        12984 c        11944 g        11886 t
ORIGIN      
        1 atcatatctc ctctttattg aaaaaatcct catctagtat ggagtccgat ttttcttttg
       61 aaataaatga tccatctata tgtccttagg attctgaaaa taaaaaattc taatctcact
      121 actcgttggt attaatttca ataaaaggaa agataaatcc tccgatggac gacttaaccc
      181 aacgctacta cgaagccgaa atgcgctacc tgcgtgaagc ggggaaagaa tttgcgcagg
      241 cttaccccga ccgcgccgcg atgctcaatc ttgataaacc cggcgcacgc gatccctatg
      301 tggaacgcct gttcgaaggc tttgcttttt taatgggcag cctgcgcgag aagctggacg
      361 acgatctgcc ggagctgact gaagggctgg tcagcctgct gtggcctcac tacctgcgaa
      421 ctatcccgtc actttccgtg gttgaactgt cgacagatca ccggcagatg aagcagagcg
      481 aaacgctgag cgattttcag gtattgtcgc gccctgttgg cgagcgcaga acccgctgtt
      541 tctacagcgc cacgcgggat atcacgctgc atccgctggc gttgccggac gtgtcattgc
      601 aatatgaacc ggacggacgt tcggttattc gtctgcgctt tgaatgcggc ccgctggtcg
      661 gtgactggtc acagatcgat ttatcccgcc tgccgttcta tctcaatgcc gacagtccgg
      721 tggcctgtgc gctgcaccgg gctttaacgc ttggtacgca gcagttctgg ttgcgtctgc
      781 cggggcagga tcgccggacg cttgacgcac acttttcccc gttgggcttt gaggataatg
      841 acaggctctg gccaaaaggc gagtcggcgt tcagcggcta ccagttgctg ctggagtatt
      901 tcaccttccg cgagaagttt atgtttgtgg cactcaacgg gcttgagctg gttgcatggc
      961 cggaggggat aaccggcttt gagattgacg tggtgctgaa cgagaactgg cctcacgacc
     1021 tgccgtttga cagcgataat atccgtctgc actgcgtgcc ggtcatcaat ctgttcccgc
     1081 ccgaagccga tccgttacac ctttcgccac tggaaaacga atttctgcta cgcccgatgc
     1141 gcattcagga cgggcatacc gagatttatt ccgtcgacaa cattatttct tcgcgccata
     1201 ccggcagtca ggcgtatgtc cccttctcca gctttcgtca ccggggcggg atgctgcgcc
     1261 acgatgcgcc ggaacgctat taccacacgc gggtaaagcg cggcccttcc ggtctgcatg
     1321 acacctggct gattctgggc ggtgatgcgt ttgatgctga ccggatgctg gaggatgaaa
     1381 cgctctcttt aagcctgacc ggtactaacg ggcatcttcc ccgtaaggct ttacagagca
     1441 cgctgctcga taggccagtt catgcctcac agaacgtcct gcgggtgcgc aacctgtgcg
     1501 ccccaacgca gccctgctat cccccggcgc gtgaccgctt ccactggcgg gtgctcagcc
     1561 accttggttc taacttttta tcgatgatgg ataacgccga aattctgcgc ggaacgctgg
     1621 cgctgtacga ctggaccgaa agcgagatga accgccgtcg tctggaggcg attgtcgacg
     1681 ttcagcacag cctgatacag cgttttgaaa gaggctttct gctgcgtggc gtggatattc
     1741 aggtgacgct ggacagtaac ggctttgccg gagaaggtga tatcacgctg tttggtgaac
     1801 tgctgcaccg ctttttcgca ctgtatgccg atatccactt attcactcaa ctgaccttaa
     1861 ttctgcaacc tacaggaaag tgcctgcaat ggacagagca tcacagccag cgcgtaccgg
     1921 gctgaccgac cggctggccc cggatattgc gcggatcaat ttttaccgtt tttgccagtt
     1981 tctggaacag agccagcaga acgcgccgct gggcagtacc gacaaccccg ccaccgatgc
     2041 ggtgcgcttt cgtccccatc ccggaatggg ctttccggtc agcgagttaa agaacgttga
     2101 acgtgacgcg ggcaatccca acgtgccacc cacggtgcgc accacctttc tcggcctgta
     2161 cggcgtggat tcgccgctgc caaccgccta tctggactac attacccagc gtcatgacgg
     2221 acatgacgca gtgatggcgt ttctcgatat ttttaaccat cgctttatca cccagtatta
     2281 ccgcatctgg cgcaaatata actacccggc gtcgtttgaa gcaggtgcta tggatgacat
     2341 ttcccgttgt ctgctgggac tcatcgggct gggtattccc ggcagcgaga accatatcgc
     2401 cacgccggta tcccgttttc tggccctgct cagcgtgatg cgcctgccga cgcgcacggc
     2461 ggaaggcgtc acggcactgg tgggactgct tgccccgctg acgaaagcga cggtggtgcc
     2521 tcacgatccg cagcctgtta ttctcccggc ccctgcgggt ctgtcaaaaa attcacgcat
     2581 cagcctgaaa acgcgaacgc tgctggggcg taccggcacc gacgtcaaca gccagttgct
     2641 gctgaaactg tataccgaag atgccgcaga agcacgtggc tggctacctg gcgggcagct
     2701 gcacagcgat ttgctggtgc tgctgcgcgt ttatctgggc tggcgctgtc aggcccgtct
     2761 gcaactgacg ctgcccgtca gcctgttgcc tgctgcccgg ctgggaaaac agcgcgtaca
     2821 gattagccgt accggcatcc ttcgcgcctc ctttgccgcg cccgcgaccg gaacagtgac
     2881 ggtcagtctg gggcgttatc agggattaat acccgcttta tccattcgta acagggagag
     2941 tatgactcat gtttcctatt cgttttaaac gtccggcgct gctctgtatg gcgatgctga
     3001 cggttgttct gagcggctgc gggctgattc agaaagtggt ggatgaatcg aaaagcgtgg
     3061 cctcagccgt tttctacaag caaatcaaaa tactgcatct cgatttcttc tcccgaagtg
     3121 ccctgaatac ggatgcggaa gatacgccgc tttccacgat ggtgcatgtc tggcaactga
     3181 aaacccgcga agattttgac aaggcggatt atgacaccct gtttatgcag gaagagaaga
     3241 cgctggagaa ggacgtactg gcaaaacaca ccgtctgggt aaaaccggaa ggcacggcat
     3301 ccctgaatgt gccgctggat aaagagacgc agtttgtcgc cattattggg cagttttatc
     3361 accctgatga aaaaagcgac agctggcgtc tggtgatcaa aagggacgaa ctggaggccg
     3421 acaagccgcg ctcgattgaa ctgatgagaa gcgacctgcg actgctgcct ctcaaggata
     3481 aataaccgat gtcgacacct tccttatatg aaatgctgac gttcagtttc agcggcgagt
     3541 taccactgga acaggtcagc gagcgggatc agcttatcct ttccgtgatg gacaatatgc
     3601 aacgcattat caactgtcgg gcaggcacac tggcgcattt gccggattac ggtctgccgg
     3661 atctcagtct gatccatcag ggtatggttg cgggtattca tggcctgatg cgtcagattg
     3721 aagaaacgct gctgcgttat gaaccacgcc tgagtcagat acaggtggag ttactccccc
     3781 agccccgtcc ggggtatctt aattacctga tccacgcgca gcttcccgat accggctgga
     3841 tacgctttga tggcgtgttt tctccggaag gacgaattgt tctgcgtcat ctcaaacagc
     3901 aggagcgggc gtactgatgg caagtaacgc gaattttatc agccagttcg tcatgggcgg
     3961 cgatccctgt acttataagg aatccggtga actacaggct gaaatgagta aactgactca
     4021 cccggcccga cctgatgtgg actggcgtcg ggtggaaaaa ctcagcctcg cgctgttccg
     4081 gcaaaatggc gtggaattac agacgcaggt ctgttacgta ctggcgataa ccagacggca
     4141 ggggctggca gggatggcag acggactcgg ttcactggac atactgctcc agcgctgggc
     4201 tgacttctgg ccggtacagg tacattccag aatatcactg ctcagttggg tcacagaaaa
     4261 aatgcagcag gcactgagaa cgctggatat tcagtatcag gatctgccgc agatttaccg
     4321 ttgtgtacag catctttccg ccatcgaaac cacgctgcaa cagtgtgaac tgtggcatat
     4381 gacgaaactg gatgtgctgg ccgggcagtt tcgtaatacg gcgctacgtc tggagcggct
     4441 ggcaccacag ggagcggaaa ccactatcac tccccctgaa ttaccccgcc gggaaatgaa
     4501 gcaaccagaa aagtcagaag aaagtccaca gccggttttt gcaaccagac ccgctcagca
     4561 aaacgataag gatgccagtc catccgcgcc atcccctgaa atctcctggc agcggacatg
     4621 gccgatattt atggccggaa tggttgtgat ggcctgtctc ggcggaacag gattatgggg
     4681 ctggtcgcag cttaatcagc cggacgcact aatccagcga atacaactgt ctgtcatgcc
     4741 attaccgcag tcgctggaga gcggcgagct ggcaaagctg gatgtaaagg ataaggcgct
     4801 gctggctcag gacagaacaa ttgcggcaag tcagatgcaa ctggagcagt taaacaaatt
     4861 gcctgcccgc tggccactgg agcagggata tcgccagcta cgccagcttg atgccctgtg
     4921 gccggataat cctcaggtca gagcgctgaa cgcgcagtgg cgtaaacagc gggagctgag
     4981 cgccctgtcc acagaggcat tgaatggcta cgcacaggcg cagagccagc tacagcgcct
     5041 gtcggcgcag ctggatgcac tggatgagcg taaggggaga tatctgaccg gttcggaact
     5101 aaaaacggcg gtgtacggca tccggcagtc gttaaaggag ccgccgctgg aagaactgct
     5161 tcggcaactg gaagagcaaa aacagaccgg agaggtttcg ccgacgctgt tgacgcaaat
     5221 tgatacccgg ttaaatcagt tgctgaatcg ctatgtaatt ttactggata cgaaggtgga
     5281 acaaagtcag taaacgtcgg gggatttatg caccaataag ggatatgtga ccttgccatt
     5341 ttttaaaagg agtctttgtg tacaaaaaag agaggaacag gccattcgaa acgcgtgaag
     5401 aggttctggc atatcataag cgggaaaaaa taccctgtct ggagtgtggc aagttgctca
     5461 tttttttacc gcgccatatc tggtttgtgc atggaatacg cgctgacgaa tatcgtgtaa
     5521 aatggaacat accgaagcac atcgcactgg cagggatttc atatctggag aaacaaagtg
     5581 aatatatgaa ggcgagaata gcaaagggaa agctggatcc cgcagaacaa atagccatga
     5641 tgagaataaa gggaaggcaa ctaaagtcag atcctgactg gcgtaatcgc tccggttcta
     5701 tattgaagaa acaatatgcc cgacgagata tccttaaaaa cagggtatgg gaaagatctc
     5761 ccagcataaa aagtgtctct gtggagttga aaacagaagc tataaggcga atgaaaaaca
     5821 ggaaggtcgt cggggaaaaa gtccaggata ttgccgatga cctgaacgta tctatcagca
     5881 gattgtatca ctggctaaag atgtaggcgc tcatgtgctc tcaatgcctt caataaagcc
     5941 gctatgcgcg aaccggcaca tctggtagac tgttctcagt gagtcaagca aactgcctac
     6001 ttttctaact tttaatcatg aataaccagc gccttgagta tagctggttc gaatatactc
     6061 gttgattcac atcagataat gagtgtataa ttcggtagat agcttgggtt cgggaacagt
     6121 gaatgcctta agtttcaaaa ggttagatta acctcttgac tgtggccccc gcaaccaatt
     6181 attgaacacc ctaacgggtg tttttttgtt tctgctctcc cataaaaaaa gcgccattca
     6241 gcgccttttt atcatcccct tctcgccagc gtcgccccat cggcaaaata cgctttaatt
     6301 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
     6361 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
     6421 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat tttaccgagc
     6481 ttattcagta ccgctttacc aaacttcagg ctgtcggcaa tggtcagcga ctgcaccata
     6541 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
     6601 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcacc tttggttgag
     6661 agcgcaaaca cagaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
     6721 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
     6781 atgtcttcat tgcgcgtcat gtacaccttc atattgccct ctttctcgat cagagagcgc
     6841 aaacggcgag ctatttgcaa taccacgtct ttttcgcgcg ttttgtattt ccccaccgca
     6901 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
     6961 gctttacctg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
     7021 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
     7081 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
     7141 tttacgtttt gctttaattc aaaaaccatc cgtacggttt gcgggtcaaa ttgcccgacg
     7201 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     7261 gagttcaggt ttacatcttc gatatccacc accacgcgtt ccgggttact caacgcgaac
     7321 tgcttatatt tcagctgacg atttgattct accgtcacac gggtgtagct ggacgcaggc
     7381 cagacgcgca ccgccacgac ctggctgacc gcagccagac tgacctgact tacgctcaat
     7441 agccacatgg cacccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     7501 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     7561 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     7621 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     7681 cgaaaaaaca gaataaaaat acactaattt cgaataatca tgcaaagagg tgtgccgtgg
     7741 taaaggaacg taaaaccgag ttggtcgagg gattccgcca ttcggttccc tatatcaata
     7801 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     7861 tctccagtat cgttaatgat atcgggttat tgcacagcct cggcatccgt ctggtggtgg
     7921 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     7981 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     8041 gaacattgca actggatatt actgctcgcc tgtcgatgag tctcaataac acgccgctgc
     8101 agggcgcgca tatcaacgtc gtcagtggca attttattat tgcccagccg ctgggcgtcg
     8161 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgatcc
     8221 atcgtcaact ggacagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     8281 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     8341 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     8401 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     8461 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     8521 tgcgtcgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     8581 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc tgagcagatt cgtcgcgcaa
     8641 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     8701 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     8761 gcgataacac gactattgcc tgcgccgcgc tctatccgtt cccggaagag aagattgggg
     8821 aaatggcctg tgtggcagtt cacccggatt accgcagctc atcacggggc gaggttctgc
     8881 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     8941 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     9001 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     9061 tagggtaagc gatgaaaatt gtcggatgcg acatgcgtaa cactcgtgcg tcgcatccgg
     9121 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     9181 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac ggcgacgcgc
     9241 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     9301 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     9361 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     9421 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatctc cattaaacaa
     9481 cccaagcgcg ctgtcattac gggcaatcat caccggtcga ccttcgtacc aacgagagtg
     9541 cggatgacga tgaattttgc gcttctgttg cataaactgc tcaattcgct cattcagtcc
     9601 agccacgcca aacggccctt cccgcagggc gcacaaaagt tggtactcat tgaacgcctg
     9661 aatgattaaa tccggctcag cacgcgcttg cagcagatcc agataacgtc cataacccgc
     9721 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     9781 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     9841 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     9901 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     9961 caggcggctt agctgcccgg cacgctcggc ggtaaagccg gcattggcat aagcgcagat
    10021 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
    10081 cactcgcgca tgatcgggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
    10141 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
    10201 acgctggcta cccggctgcg cacccagcaa tcggtgcaaa gtactggcat cttccggaat
    10261 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
    10321 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
    10381 ggccatttga attaacgctg ccagtaactt cgccacggtg gtcgttttac cggtgcctgg
    10441 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
    10501 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
    10561 aacctcaatg gcatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
    10621 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
    10681 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
    10741 gacacaggtc gccaacagcg gatgcgacgc ctcgttattt tccagtcgtg aaagcggcaa
    10801 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
    10861 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
    10921 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
    10981 gcaaacatct catccatcag gtcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
    11041 tgttgcggat gttctttatc aacaccacgc aggaacagat aaataacgcc gccaaagtgg
    11101 cgctcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
    11161 agctgatatt gcagatcata gcggtgtgcc tgcatcgccg ctgccatagc ctgttgggtg
    11221 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
    11281 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
    11341 agcggcgggc agccagcgga tagcggatca aactggcgaa ttagtgcatc aagctggctg
    11401 gcgatgagcg gttcactaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
    11461 gaaagctgat tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
    11521 cactcggtca acactggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
    11581 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
    11641 gtccctggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
    11701 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
    11761 ccacgctgct gcaaaccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
    11821 agcgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctgtttatca
    11881 cccgtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
    11941 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
    12001 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
    12061 caatgccaaa ccgaacgtgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
    12121 tcggcctccg cgaggtcgac gctttctggc gcagcattaa gatccagaac tgcctcaaac
    12181 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtgat aaacggcagc
    12241 cagaccaatg gatattccag ccctttcgat ttgtggatcg tgacaatctg caccagatgt
    12301 ttatcacttt cgagacgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
    12361 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgtagc
    12421 agttcgctga tatgcaagat atcggtaagt cgccgctcac cgcctgccgt tgccagcaag
    12481 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
    12541 cgccagattt gccgataacc atcgaactct tcgaccaccg cgtcccacgc atgttcgtca
    12601 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
    12661 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
    12721 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
    12781 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacactgatg
    12841 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
    12901 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgataa
    12961 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
    13021 cctttaaata caaaacgtaa cgcctgattt ttcccgactg atttcactgg aataaacggt
    13081 atttcgcgaa acatgaacgc gtcatcagtc tggctgaaaa gcttattcac gctgttcacc
    13141 attcctggtg cggaacgcca gttggtgtct aaagtgtagt gggcgtgaac ttcgctacgc
    13201 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
    13261 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
    13321 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
    13381 gccgctgcca acacctcacc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
    13441 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
    13501 gccaatgcgc gggtgatcac cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
    13561 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ctttcgtgcg atcttctaag
    13621 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgttggt ctcttcttct
    13681 gcccaggcgc tgatcttgtc gatccattta gcctgattgc tacggttaaa cttgcgtcga
    13741 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
    13801 tttaccgtag caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
    13861 ggcggcggtg ctttgataac cggcgcttcg ccttgcagat aacgattaat atcgcgcagc
    13921 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
    13981 tagcagtggc gacgccagaa atcggcgcag gcctggtaac gtagcagaga ctcatcttca
    14041 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
    14101 caaaaaccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
    14161 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca gacgttcgta cagtggattg
    14221 tcggtggttt cacgcagaca ggcgatgcgc aactcatgga tattgctacg gatacgacca
    14281 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacggtcagc
    14341 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
    14401 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcacc ctgtaagggc
    14461 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
    14521 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcagggt gtacatattc
    14581 ggctttccca ttctggctgc cggaaatctg cgacaggata gccatgcctt gcggctcgac
    14641 taccgcctga tggaagaaat cggccagttt ttgcggcgtc agcagtttaa tctgggccac
    14701 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
    14761 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
    14821 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcagttg ggaaaaacgc
    14881 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
    14941 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
    15001 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
    15061 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
    15121 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
    15181 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
    15241 tgcctgggct tcggtcatgt tgccaataac cataaactct ggtcgagccc ctgattttaa
    15301 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tgcgccgttc
    15361 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcgaa
    15421 cgctttgccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
    15481 ctgatcttcc gtggcggtat agctaaagta gccttccagc aatgcctgga acagctgcgg
    15541 cagacgctgg gtgtaaccat tagcattaac cataaggccg ttgttagcgt tggtggaaaa
    15601 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
    15661 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
    15721 aatcaggctg acatcggctt ttggctcgct ggcaaaataa cggcttggcg catacaccac
    15781 gcgcagattc gactcatcaa caatcagctc tggatggtca tatttcttgt ctgacttaat
    15841 cagcgagaaa tcgtcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
    15901 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
    15961 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
    16021 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
    16081 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
    16141 ttcgacgtaa tccatatcac gggtgattga cggataacgg aagtcgatat ccagcacatt
    16201 cgccagttca tcgaagtatt gtttatcgat ccctttttca cgtaacaaat tgaggtagct
    16261 aaaaatagcc gccacaacct gatcgcgatt cgccagacct ttatcggtta aagacgcaga
    16321 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    16381 aactaatccc tgcttttgca gccagtcgga aagtgtacct gggctgcgat taccaatcag
    16441 ataggtaatc aattcatcgg ttttgctacg gaatttcgcc gagttgttat caatacgaaa
    16501 ctctacgcgc aacactttac gcggcagcgc cgggacgtaa tgaatgataa tgcccttttg
    16561 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    16621 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattgc tgtaaataac
    16681 cgccttcatc agattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    16741 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    16801 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    16861 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    16921 caagggttct gcaatagcat cagccaggcg gtctaccgca ccaggcaagg cgtcgttctc
    16981 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    17041 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    17101 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    17161 gggcaccacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    17221 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    17281 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aagggtgccc aaagggcaac
    17341 taacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    17401 taattttctg tccagattca acacgttaac gcaaatcatc tgccagaatt taatcttgtg
    17461 ctgcacgagt cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    17521 caagcggata gattgcgcaa tttttataca gcactcatga ctgattaaag cgaaacagcg
    17581 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    17641 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    17701 aagcctgcag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    17761 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    17821 atggcgcgga catcccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    17881 gttcggctgc aagcggcgga aaacgccact cgccgtcttt gcgtagaaaa aggcgacttt
    17941 caccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    18001 ccacacttaa taaagaggga cgccagcgca acaagccatc cggctgcacc tgcggcaacc
    18061 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    18121 gacaggcaat gactctgtcg gcaagctgct gcatctcatg gcactgtgtt tcccagaaaa
    18181 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    18241 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacgactaa
    18301 gcccttcaag aataaatggc tcggtgtcgg ggatttcgct gtcttcagta cggaagttca
    18361 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    18421 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    18481 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    18541 ggtagttttg tggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    18601 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    18661 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    18721 agcgcccgat atagctgata taaagttttt gttgcgcgga aattaacgct tccaggaaca
    18781 gatagcggtc atcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    18841 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    18901 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    18961 gaaaacgctg gctgatacgt tcttgatcca gacgctgcgc cagttcatca cgcaatagtg
    19021 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    19081 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    19141 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    19201 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    19261 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    19321 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    19381 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    19441 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    19501 ccagtaatgc cagcacgtct tccgacacaa agcggctgtc tggcaacgat aacaggctga
    19561 taaacgcttc cagtaccgga tgcgactgcc gcgcccgacg gtcggaaatg gcgtaaggca
    19621 ggtaacgatc cgcaggtgcg ctaccaaaca cagcctgaat aaacggactg tagctgtcga
    19681 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cgggtcttct tccagcatcg
    19741 ccagcaggcg atcgtgcaaa acttcaactt cacgctgcgg gctatggcaa acgtggaagg
    19801 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    19861 tgttcacacc agcaacggca cggttttcca gttccagaat gtcagactga atgttatgca
    19921 gcaggttatc tggcgtgaca tcgacaaagg cgtccagctc ctggctgctc tccaggtcag
    19981 aaaggagata aatgtagtcg cgcccaagct taccccatga agccagcagc gggttgccga
    20041 catcctgttc accatcgctg ttaaagagct gcccggcatt ttcgctatca cgaaatagcg
    20101 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgggt cagcagtttc gccagataag
    20161 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    20221 caatatgttt acccagcgcc tgtagcgcct ggagataaac aggcggtaac gcggaaatac
    20281 cgcatataaa gacgcgcgaa ggtagccccg gcgggcaggt cgtcgcggac tccagcgttt
    20341 cgataaagcg ctgatagaga ttggcgcggt gccagcgcgg ttgcccgagt tgatgggtat
    20401 attccaccaa cgccttccac aacggcgctt gccaggcctg tgcttctccc agcccttcaa
    20461 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    20521 acaggtccgc cgcttttgaa gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    20581 gataatgccg caacagggta aagtcttcgc gctccaacaa ttgcggcagc agagtcatca
    20641 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    20701 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    20761 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    20821 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    20881 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    20941 gacgattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    21001 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    21061 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agctgttggt
    21121 actggtttct gctggcgaga ctgttcatta atgttcgctg gatacccgat aacgcagtga
    21181 caatcatcac catcagcacc atcgcaacca ttacctccgg caggctaaag ccttgttgat
    21241 tcttcaggga agctgacata acgccctctc tttcagcgga caaaaatcgc tccagccgcg
    21301 tggcgaaaaa acaatgtttc cattaatgac ttcgcctgtt cgccacaacg aaacgccttc
    21361 ataaccggca atcaataagg cttcattatc tgccagtaaa cgcaaacaaa cctgggcatc
    21421 ggttccagcg tactgcgagc actgaactgc tggctgcgtc tgccaggagt gcatttttcc
    21481 ccacgccagc gccgactgaa cgatggcctg gcggcgcaat gactgacttt ccatgctcac
    21541 gcgagaggca aaactgcgat cctgctgact cattccttgc aatagcaagc tacccaaaac
    21601 cagcagcatc aggaccagag ccagtgacga aacacctttt tcgcggttca caggttgaat
    21661 cctgtcacgc tatagctggc atccaccacg gtttgcggtt cagacttact ggcagcacgc
    21721 atattaaccg tcaacaccgg cgagaagccg ctgacatcct gacgtacgac ctgaaaagtg
    21781 tcgataatga tggcatccgg attagtgact ttatcccagc ccttaccttc acaggatgtc
    21841 gcaccgcgta gcgtttccag cacatgctcc ttcagacgaa atccaatctg gtcagactct
    21901 tttaccggtt cgcgatccca gataccgtta ctgttcgcat cccactgcac aatgatacag
    21961 tcaccctgtc cgacaatttc cagcccttcg ccggtacagc tgccatgaca ataacccgcc
    22021 ctctggagat gcttcgcgac ggtaaatacc cgcagccaga tttcatcttc cagcgccagc
    22081 ttacgggtgc tcgttaaact ttcacgctgt aacgcaggca gaaagcgtgc agcccccagc
    22141 aacaatacgc tactgatcgc catagcaatc aacacttcca gcagagaaaa accttgctct
    22201 tttacaggca tccttctgtt tctccttgct gacaaagccg gagtcttccc cacggcgaaa
    22261 ccaccagcca ccactcgccc gttgagtttt tgaagcgaat atgcccggcc catgcggtat
    22321 tgcgcaggcc aaagaaagca agcgaaggtg tcaggtcgct catttcgact tcgggccagc
    22381 gtggcacaaa gaccaatggt gaactgccat gacaggtatt ggccccagca gcggaactca
    22441 caaggcacca taacgtcccc tccctgataa cgctgatact gtggtcgcgg ttatgccagt
    22501 tggcatcttc acgtaaatag agcaaatagt cccgcgcctg gctggcggtt tgccatagcc
    22561 gttgcgactg ctgccagtat tgccagccat agagtccact tgcgcttagc atgaccaaaa
    22621 tcagcatcgc gaccagcgtt tcaatcagcg tataaccacg ttgtgttttc atgccggcag
    22681 tatggagcga ggagaaaaaa agacgagggc cagtttctat ttcttcggcg catcttccgg
    22741 actatttacg ccgttgcagg acgttgcaaa atttcgggaa ggcgtctcga agaatttaac
    22801 ggagggtaaa aaaaaccgac gcacactggc gtcggctctg gcaggatgtt tcgtaattag
    22861 atagccaccg gcgctttaat gcccggatgt ggatcgtagc cttcaatctc aaagtcttcg
    22921 aaacggtagt cgaagatgga ttcgggttta cgtttgataa tcaacttcgg cagcggacgc
    22981 ggttcgcggc ttaattgcag atgagtttga tccatatgat tgctgtacag gtgcgtgtcg
    23041 cctcccgtcc agacaaaatc gcccacttcc agatcacact gctgcgccat catatgcacc
    23101 agtaacgcgt agctggcaat gttaaacggc aggccgagga aaacgtcaca ggagcgctga
    23161 taaagctggc aagagagttt gccgtctgcc acatagaact ggaagaacgc atggcacggt
    23221 gccagcgcca ttttatccag ttcgcctacg ttccacgctg aaacaataat gcggcgcgaa
    23281 tccgggtcgt ttttcagctg gttcagtacc gtagtgatct ggtcaatatg acgaccatcc
    23341 ggcgttggcc aggcgcgcca ctgtttacca tacactggcc cgaggtcgcc gttttcatcg
    23401 gcccattcgt cccagatggt gacattgttt tcgtgtagat aagcaatgtt agtgtcgccc
    23461 tgcagaaacc acagcagttc atggatgatg gaacgcaggt ggcaacgttt agttgtcacc
    23521 agcgggaatc catcctgcag gttaaaacgc atctgatgac caaaaatgga aagcgttccg
    23581 gttccggtac ggtcgttttt ctgtgtgcct tcgtcgagca ctttttgcat cagttctaaa
    23641 tactgtttca tggttcctca ggaaacgtgt tgctgtgggc tgcgacgata tgcccagacc
    23701 atcatgatca cacccgcgac aatcatcggg atggaaagaa tttgccccat gctgatgtac
    23761 tgcacccagg caccggtaaa ctgcgcgtcg ggctggcgga aaaactcaac aatgatgcga
    23821 aacgcgccgt agccaatcag gaacaagcca gagacagctc ccattgggcg cggtttacga
    23881 atatacaggt tgaggataat aaacagcacc acaccttcca gcagcagctc gtaaagctgt
    23941 gatgggtggc gcggcagcac accgtaagtg tcgaaaatgg attgccactg cgggttggtt
    24001 tgcagcagca aaatatcttc tgtacgggag cctgggaaca gcatggcaaa cgggaagttc
    24061 gggtcaacgc ggccccacaa ttcaccgtta ataaagttgc ccagacgccc ggcaccaaga
    24121 ccaaacggaa tgagtggtgc gataaaatca gaaacctgga agaaggaacg tttagtacgg
    24181 cgggcaaaga taatcatcac cacgataacg ccaatcaggc cgccgtggaa agacatgccg
    24241 ccgtcccaga cacggaacag atacagcgga tcggccataa actgcgggaa attgtagaac
    24301 agaacataac caatacgtcc cccgaggaag acgccgagga agcccgcata gagtaagttt
    24361 tcaacttcat ttttggtcca gccgctgccc ggacgattcg cccgtcgtgt tgccagccac
    24421 attgcaaaaa tgaaacccac cagatacatc aggccgtacc agtgaagcgc cacgggtcct
    24481 attgagaaaa tgaccggatc aaactccgga aaatgcagat agctactggt catctgtcac
    24541 cacaagttct tgttatttcg ctgaaagaga acagcgattg aaatgcgcgc cgcaggtttc
    24601 aggcgctcca aaggtgcgaa taatagcaca aggggacctg gctggttgcc ggataccgtt
    24661 aaaagatatg tatatgatcc gcgctataac cctccgcgaa tcagcccgcc catgccgcga
    24721 cgctccataa aggctgcaac ctgatggcga acttcggtcg ccagttgcgc ttccagacta
    24781 cgctgcgcaa gattttctgc ttcggcataa tcaatgcgcc gcagcaggta ttttacccgc
    24841 gctacagaac gtccgttcat agacagatgg cgatacccaa gcccaatgag gattgccacg
    24901 cacatgggat cgcccgccat ttcaccgcac aaacggagat cgattccatg tatttccgct
    24961 tcccgggcga tcatcgccag agctcgtaac attgcaggat gaagactgtc ataaatgttc
    25021 gccacccggg tattgttgcg atcaacggcc agaatgtatt gagtcagatc gttggtgcca
    25081 acagagatga aatcgacccg ctttgccaga tgcggcagca taaataccat tgacggcact
    25141 tccagcatga tgccgatacg tggtttggga atttcgtaac cgatcatctc ctcgacttca
    25201 cgtccggcac gttcaatcag gcggcgtgct tcatcaactt catcgaggct tgtgaccatc
    25261 ggcaacagaa tattcaggtt gcccgtagcg gcattagcac gcagcatcgc ccgcacctgg
    25321 atcaagaaga tctccggctg atcgagcgta atgcgaatcc cacgccaacc caggcatgga
    25381 ttctcttcgc tgatcggcat gtaaggcagc tgcttatctg ctccgacatc cagcgtacgc
    25441 aaggtgacgg gtttatcatt gaacatttgc agcatcccct gatactgcgc cacctgttct
    25501 tcttccgacg ggaaaccact ttgcagcatg aatgggattt cagtgcgata aagaccgatg
    25561 ccatcaatac ggctgcccag tttttcttca tgttccgggc ttaaaccagc attgagcatg
    25621 acttttatac gctcaccgct ttttaactgc gcgggtaaat tgacgtcatc ttccgccaga
    25681 cggctaagct caatctcttc actaattagc cgctgatatt cttgcagcag taccggctcc
    25741 ggatcgacca gcaattcacc gcgatagcca tcaacgatca gcgtcctacg atgcagcacc
    25801 gaaggctgaa tatccgcgcc catcacggta gggatcccca gcgcacgtac catgatcgca
    25861 gcatgggagt tggctgcgcc atctcgcacg acaacaccga ctaagcgatc ctggggcagc
    25921 tcagcaagag ttgtcgctga cagttcatcc gccaccagaa tgaaacgttc cggccaggcg
    25981 ttcggccctt gattagcgtc atcaagatga aacagcaatc gctgacccag cgcacgtaaa
    26041 tcgccagccc gctctttgag atagttatcg cttagcgcgg caaactgttc ggcaaatttt
    26101 tcaatgaccg tttttaccgc ccactctgcc accgagcctt tatcaacctc ggcaaacaat
    26161 tcgcgacgca gccgggtgtc cgaaagcagg tgcgagtaaa gatcgaaaat agccgccgtt
    26221 tctttttgtg caccggcggc aaagcgtttg ctgtagcggc gaaactcgtt tgccgcttct
    26281 tccagcgccc cggtcagtcg ttcgcgttcc agagccggat ccagcgttga tgcctgatac
    26341 acctgttcca ttaaaggtaa cgtggcatcc tgccagcctt cggcaatcgc cacaccaggt
    26401 gctgccggta atgcgcggat tcgcgtctgg cgatattgcc caaacagggc agtcaactgc
    26461 gactgagaaa gaatagctgc catctgggtg gcaagcgtca ccaggaagga ttcttcactt
    26521 tcgtcatact ggcgcaactc tcgttgctgt accaccagta caccaagcaa ctggcgacgt
    26581 tgaataattg gtacgcctaa aaacgcgcgg aaacgttctt cttttacgga ggggatgtat
    26641 ttgaagctgg ggtgcttttg cgcatctgca aggtttatcg gttccgccag cctgccaacc
    26701 aggccgacga tcccttcatc aaacgcgagc gttacagtgc gaccgcgtgg ttttttcagc
    26761 ccccgggtcg ccatcaggta gtaacaacgt cgatcatgat cggccaggta gaccgaacag
    26821 acctcggtat ccatcgcaag acagatgtcg gtaaccagaa tatttaacgc ctcattcagg
    26881 cgtggtgcgc tggctacctt ttcgactatt tcgcgcaggc gagtgagcat aattggcgtg
    26941 acttaacctc ttttacgtcg ataagcagat gcgttttgtg gttttggcgt attttcctgc
    27001 agtgacatca ccacactcgc gaactctttc attaccctac ggtagacatc acgtttaaat
    27061 gacaccacct gtctgaccgg ataccagtaa cttacccatc gccagccgtc aaactctggt
    27121 gtactgctgg tttgcatatt gatttctgca tcgccgctca ccagctgcaa gagaaaccat
    27181 ttttgttttt ggccgataca aaccggcttc gtgtcccaac gcaccaaacg tttcggtaat
    27241 ttgtagcgca accagttacg cgttgaagca aggattcgaa cgtctttgcg gcttaatcct
    27301 acttcttcaa acaattcacg gtacatcgcc tgctctgcgg attctccggg gttgattccg
    27361 ccttgcggaa attgccagga gtgctgacca aatcgccggg cccacattac ctgcccctgg
    27421 cgattacaaa tcacgatacc tacgtttggg cggtagccat cgtcatcaat caccggacta
    27481 cctcaaaata aagctttata tacgaatgat tgtttcatac tccaggaaga cggtaaacca
    27541 ctctctgcag ggcattacac actaataaca attgaataac tcacagttat gtgcagagtt
    27601 ataaacagag gaaggggtgg atagccgttt ttattcacct tttctgtgga tagagttgtg
    27661 aagaactacg gaattactac gggaaaaccc ggagcattct gaataagccc gatatagaca
    27721 ttttaaaata ttcattatta gtcaatgcat tacatgtaat ttgagtaata aaaaagagta
    27781 attcgtgacc caggtcacac ctctcattta cgggttggcg aaagatcaac caatgccgta
    27841 tttatccaca gaatgtgcca ctaagttaag cactgaacca ctaaaaactg gagtttcatc
    27901 gcacgtcaag gctgtaaatg gaaacagtag tggaggtttt tcacagttat cccagctttc
    27961 tgtggataac atggtgtaag atcctgttta ttttcagtga ccagatttgg aaaacctatt
    28021 gcagtgttgc gcaactcgat taccggcagc ctaaaaagca atataaatca gggaattgaa
    28081 caacacatgt ggaaaaagtt acactgcgaa tattcggcac ataattgctg tttgtttttt
    28141 aatcaaggta tcatgacatg tcccaacctc gcccactgct ctctcctccc gaaactgaag
    28201 aacagttgtt agcgcaagca cagcaacttt ctggttatac attgggagaa ctggcggcac
    28261 ttgccgggct tgttacgcca gagaatttaa aacgcgataa gggctggatt ggcgtgttac
    28321 tggagatctg gctaggtgcc agcgcaggga gtaaacctga gcaagatttt gccgctctgg
    28381 gcgtggaact taaaactatc cctgtggata gtcttggtcg tccgctggaa acaacattcg
    28441 tttgtgttgc cccgttaacg ggtaatagcg gggtgacctg ggaaaccagc catgtgcgcc
    28501 acaagctcaa acgcgtactg tggataccgg ttgaaggcga gcgcagcatc ccgctggcgc
    28561 agcgtcgcgt aggatcaccg ttgctgtgga gcccgaatga agaggaagac cgacagctac
    28621 gcgaagactg ggaagaatta atggatatca ttgttctcgg tcaggttgag cggatcaccg
    28681 ctcgtcacgg ggaatattta cagataagac cgaaagcagc gaatgcgaaa gcgcttaccg
    28741 aagccattgg tgcccggggc gaacggattc tgacgctgcc acgcggcttt tatttgaaga
    28801 agaatttcac cagtgcacta ctggcccgtc attttctgat ccagtagcca tcgctttgac
    28861 ctgccgcttt ccgggcatat aattaccgct tcattttttt ggcagggctt tttagatgtt
    28921 atttgcatgg ataaccgatc ctaacgcctg gcttgcgctc ggtacgctga cgctgctgga
    28981 gatcgttctt gggatcgaca atattatttt cctttctctg gtggtggcaa agcttcccac
    29041 agcacaacgt gctcatgcgc gccgtctggg gttggcggga gccatggtta tgcgtctggc
    29101 gctgctggca tcaatcgcct gggttacgcg cctgacaaat ccgcttttta caatattcag
    29161 tcaggaaatt tctgcccgtg atttgattct gcttctgggt ggcttgttcc ttatctggaa
    29221 agccagcaag gaaatccacg aatccattga aggtgaagaa gaagggctga aaacacgcgt
    29281 ttcatcattc ctcggcgcta tcgtgcagat tatgctgctg gatattatct ttagcctcga
    29341 ctcggtgatt acggctgtgg gtctgtcaga tcacctgttt attatgatgg cagccgtggt
    29401 gattgccgta ggcgtgatga tgttcgccgc gcgctcgatt ggtgattttg tcgaacgcca
    29461 tccttcggta aaaatgctgg cgctctcttt cctgattctg gtgggcttta ccctgattct
    29521 ggaaagtttc gacatccacg taccgaaagg ttacatctac ttcgcgatgt tcttctctat
    29581 tgcggttgaa agcctcaact tgattcgcaa caaaaagaat ccgctctgat actccgtacg
    29641 ctctcctgcg ggagggcgta ctcactcctt ttaccctcac agtttaagat tttcctgctt
    29701 tcaagatata taacgtcggt ttataaacag actattatca taggtaggcc aaatgaggat
    29761 aaccggatga aaaaatgggc agtaataatt tctgcagtcg gactggcgtt tgctgtttcc
    29821 gggtgttcca gtgattacgt catggcgacc aaagatggcc gtatgatttt gaccgatgga
    29881 aaacctgaaa ttgatgatga taccgggctg gtgagttatc acgatcagca aggtaacgcg
    29941 atgcaaatta accgtgatga tgtttcgcaa attattgaac gttaacaaat aaggtcagca
    30001 tccggctggc cttaagattt ttctctttcc cttttccctt ccctctgcca tttttatatt
    30061 ccttatgtcg tgattataaa aaggaaacgg ctatgcaata tcaccgtata ccccacagtt
    30121 cgctggaagt cagcacgctg gggcttggca cgatgacgtt tggtgaacag aacagcgaag
    30181 ccgacgccca cgcacaactc gactatgccg tcgctcaggg cattaacctt atcgacgttg
    30241 ccgaaatgta cccagtacct ccgcgccccg aaacgcaagg gttaaccgaa acctacgtcg
    30301 gcaactggct ggcgaaacat ggcagccgcg aaaagttaat tatcgcctcc aaagtgagcg
    30361 gaccgtcgcg caataatgac aagggcatcc gcccggatca ggcgctggat cggaagaata
    30421 tccgcgaagc gctgcatgac agcctcaagc gcctacagac tgattacctc gatctttatc
    30481 aggtgcactg gccgcagcgc ccgaccaact gcttcggcaa actcggttat agctggacgg
    30541 attctgcgcc tgcggtttcg ctgctggata cgctggacgc actggcagag taccaacgcg
    30601 cgggaaaaat tcgttatatc ggcgtgtcga acgaaactgc atttggcgta atgcgctacc
    30661 tgcatctggc ggacaaacac gatctgccgc gtattgtcac cattcagaac ccttacagtc
    30721 tgttaaaccg cagttttgaa gtaggtctgg cagaagtcag ccagtatgaa ggggtcgaac
    30781 tgctggccta ttcgtgcctg ggtttcggca cgctgaccgg gaaatatctc aatggtgcaa
    30841 aacccgctgg cgcacgtaat acgctcttta gtcggttcac ccgctatagc ggtgagcaaa
    30901 cgcaaaaagc cgtcgcggcg tatgttgata tcgccagacg tcatggcctg gaccctgctc
    30961 agatggcgct cgcgtttgta cgccgtcaac cgtttgttgc cagcactctg ctgggcgcaa
    31021 ccacgatgga tcagctgaaa actaacatcg aaagtttgca tctggagtta agcgaagacg
    31081 tattagctga aattgaagcg gtgcatcagg tttatactta tccggcacca taagattaac
    31141 gccggttaaa accggcgtta aatattaatg acggcgctgc cagatccaca gcgccgttat
    31201 tgccagcgca aacagcgcac cgaagccaat gccaatgggc acgaccggga tgcctatcat
    31261 taccgccagc gagtaaatgc ccagcatcaa caacatggcg ctgttttcgc caaggttttg
    31321 tactgcaatc gcattccccg ccccgacgct ttttttaccc cgctcctgta gcaacgcatt
    31381 gagcggaacg acaaaaaagc cccccatcac gccaatcagc atcaacaagg cataggctgg
    31441 cagcagctcg tgttgcaggg aaaaaatcag taccaccacg ccaatcaaaa tcccggctgg
    31501 catacagcgt gacacggttt ccagcgtaac taacttcgct gccgcacctg cgccaaccac
    31561 gataccaatc gctaccatcg cgttgagata ggtgggcgta gcgttatcgg taatgcccag
    31621 cgccaccggt acccacagca ccaacaggaa acgcagcgtg acacccgctc cccagaataa
    31681 actggtgccc accagcgaaa aacgcgtttc accattgcgc catagcgagg tgcaggcatt
    31741 caggaaactg cgggtcatgt tgatgagatt ccaggactgc cccggacgcg ccgccgccag
    31801 tttgggaatg tagatattgg caacgaccgc accaccgtag gccagtgcgc atgcggccag
    31861 ggcgacgagg acatgccagt cagccagcac accaccggct acggaaccga gcaaaatcgc
    31921 cgctatggta gaagcttcca ttaaaccgtt agctttcact aacttactac ccgtggttaa
    31981 ttcgccgaga ataccgtatt tcgccggtga ataggctgca gcaccaacac ccaccagcgt
    32041 atagccgaga aacggattga taccaaagca gatactggct gcgcccagca gcttcaggcc
    32101 gttggcaaac atcatcaccc ggcctttggc gaagctatcc gccacctgcc cgacaaacgg
    32161 cgcaaaaaga atgtaagcac ctacaaacac catttgcagg atgggctggc tccactccgg
    32221 atagaactgc gctttcagta acgccagagt ggcaaacagt agggcattat cgccaaacgc
    32281 agagagaaac tgcgccacga taaccgcttt catccccttc gaccacaacg aagtgttagt
    32341 gtgcactgac tcactcatcg tgttgttccg cttcgtctac ccagcttttc aacgtgacaa
    32401 agtcaggttt gccgctgcca agtaatggca tctgtttcag atagcgaata tcgcgcggta
    32461 cagcaagctc cggcacgccg tgctcgcggg catactgttg caacttatcg cgcgtcagtt
    32521 cgttatctgt ggtgaaaagc accagtgcct cgcctttgct ggcatcgctc ttaatcgcag
    32581 tggcatggac tttatctggc gaaacaccaa gtgccagttg ttccaccatt tccagcgaca
    32641 ccatttcgcc tgcaattttg gcaaagcgtt ttgcgcggcc ctgaatctgc acaaagccct
    32701 gctcgtcaaa acgcacaata tcgccagtgt cataccagcc gcgctccatt tcgccgcgaa
    32761 cattctcggc ggtgggcact tccagtacac ctggcttctc cacccgcaga tagccgttca
    32821 ttatgttcgg ccctttcagt tgcaggcgtc cgccctcttc gataccaggg accgacaaca
    32881 ggcgcgcatc cattcctggt agaatacgcc ctaccgtacc gggtttcgcc gccatcggta
    32941 cgttgataga aacgacaggc gcgcattcgg tcacgccgta gccttcaagg atgcgcaggc
    33001 caaatttatc ctgccaaagc tgtttggtac tttcttgtaa tttttctgcg cctgccacca
    33061 catagcgtag acgatagaag tcatacgggt tggcgaaacg cgcgtagtga ccgaggaaag
    33121 tcgaggtgcc gaacaacacg gtgcaactgc ggtcatacac caactccggc acaatgcggt
    33181 aatgcagcgg gcttggataa aggaacactt ctgcacctgt aagcagtggc gtaaacaggc
    33241 ctaccgtcag cccaaaggag tgaaacagcg gtaacgccga cataaagcga tcgttggtgg
    33301 tgaagtcggc aatcgtttta atctgctcga cattcgccag aatgctttta tggctatgga
    33361 cgacgccttt cggatggcct tcagaaccgg aggtaaagag gatcagcgcc tcttcttccg
    33421 gctgctgttt aacctgtgcc agacgcggca tcagcaaatg agcgaagatc catactttgt
    33481 cggcagtggt gacatctgct tttaaatctt ccagatagac ccagcgcacc tgagtaagtt
    33541 gctccggcag atgccagagt ttgcctttat cgagaaactg gcgggaagtg aagatggttt
    33601 tgatttcagc cgccgtaata gcactggtca gcccttttac cccggcagtg tagttcatca
    33661 ttgcgggcat gcggcgacgg gcgatggccc caaaaatcac tgccgcactg atgcctgcat
    33721 tgggcagcat taagccgatg cgttcgcctt caacactgta tttttcaagg atgcgtccaa
    33781 caaacagcgt tttcgtaagc aatttgcgat aggagtctgg ggtaaagttg acgtcttcga
    33841 cacatttctt cccggctccg aagcggtaca ttgcactcag taaagattcg tacagcgttt
    33901 cacgcgggcg caccgccatt cgcgcttcca tcattatttg atgcagcatt tcgccagcga
    33961 ttttgcgacg gtcacgggca cgcggcgcat ccggcatcgc cacctgcgtt ggtggcaaaa
    34021 tatgcagagt aatttgcggg aacaagcgac gtttaaccag acctttcagg cggctgaagt
    34081 gcgtaagttc cgccccttca atacgcacag gaataaccgt tgcaccagac ttcgccgcga
    34141 caaaacccgc gccatcgtag attttcatca gcgagcctgt agtggtgatg cgtccttcag
    34201 ggaaaatcac cactggtcgg ccctgttcca ccagacgtac cagatgttta atagccatag
    34261 gttgcgtcgg gtcgagagga acaaagtcga taaatgattt cagccaacgc atataccact
    34321 gttggcttat tgaggtgtaa acggcaaaca ctggacgcac aggtaaaaac agtccaagca
    34381 aaatgccatc aataaaagag acgtgattag gcgtaattag aacgcgctcg cccttcagtg
    34441 cctgggtgtc acccgtaacg cgaacgcgat acaaaacacg gcacaaattt cgaaaaaagc
    34501 taaaaagcat tccaactccc tttgctctga ttcagtaaaa gcgaatggag ggagattaca
    34561 cgagataaag aacgcgagcg acagtaaatt aggtgcgaaa aaaaacctgc gcatccgcgc
    34621 aggttggtgc aagagacagg gtacgaagag cgtaccgaat aatctcacca atcaatacct
    34681 ctgggatctt gattgtggtc tgcacgacga ctcttcgcca gcgagaaaac gcaaaggaat
    34741 gaagggaaat gcaacgaggt gtgtaaattg tcggttactg ttacagattg atgaccggca
    34801 aaaaaaaacc tgcgcatctg cgcaggctgg tgtaattcat gtgctcaacc cgaagttgac
    34861 ttcacctatc aatacctctg ggatcaccac tttagcaacc tgaagccaaa cgccaccagc
    34921 ggtcaatcgc aacagcgttt cgcaaagtgt aaccaaaggt ttgcaatctc tttttttgtc
    34981 ttacttattt gcgtttggct cacattccca cgatgaaaac acgccacccc ttgaaccaac
    35041 gggcgttttc cgtaacactg aaagaatgta agcgtttacc cactaaggta ttttcatggc
    35101 gaccataaag gatgtagccc gactggcagg cgtttcagtc gccaccgttt cccgcgtcat
    35161 taataattca cctaaagcca gtgaagcttc ccggctggct gtgcatagtg caatggagtc
    35221 tcttagctat cacccgaacg ccaacgcccg tgcgctggcg cagcagacca ctgaaacggt
    35281 cggtctgatc gttggtgatg tttccgatcc gtttttcggt gcaatggtga aagcggtcga
    35341 acaggtggct tatcacaccg gtaatttctt attgattggc aacggttacc acaacgaaca
    35401 aaaagagcgt caggctattg agcaactgat ccgccatcgc tgtgctgcgt tggtcgtcca
    35461 tgccaaaatg atcccggatg ccgatttagc ctcattaatg aaacaaatgc ccggtatggt
    35521 gctgatcaac cgtatcctgc ctggctttga aaaccgttgt attgctcttg acgatcgtta
    35581 cggtgcctgg ctggcaacgc gtcatttaat tcagcaaggt catacccgca ttggttatct
    35641 gtgctctaac cactctattt ctgacgccga agatcgtctg caagggtatt acgatgccct
    35701 tgctgaaagt ggtattgcgg ccaatgaccg gctggtgaca tttggcgaac cagacgaaag
    35761 cggcggcgaa caggcaatga ccgagctttt gggacgagga agaaatttca ctgcggtagc
    35821 ctgttataac gattcaatgg cggcgggtgc gatgggcgtt ctcaatgata atggtattga
    35881 tgtaccgggt gagatttcgt taattggctt tgatgatgtg ctggtgtcac gctatgtgcg
    35941 tccgcgcctg accaccgtgc gttacccaat cgtgacgatg gcgacccagg ctgccgaact
    36001 ggctttggcg ctggcggata atcgccctct cccggaaatc actaatgtct ttagtccgac
    36061 gctggtacgt cgtcattcag tgtcaactcc gtcgctggag gcaagtcatc atgcaaccag
    36121 cgactaaccg cagttaaagc aattccagcg ccagtaattc ttcgatggtc tggcgacggc
    36181 gaatcaaccg cgcctgacca ttatcaaaca gaacttctgg taacagcgga cggctattgt
    36241 agttggatga cattgatgcg ccatatgccc ctgtatcatg cagtaccaga taatcacccg
    36301 ccttcacttc cggcaaggcg cgggtttcaa catttccccc ttcctgctgg gtaaagacat
    36361 cgcccgattc acataatggc ccggcgacga cggtttccac cgttggcgcg tgttccagag
    36421 aacgaccatc agctgccagg gcactgatat ggtggtaact accgtacatt gccgggcgca
    36481 tcagatcgtt gaacccagca tcaaccagca caaagtggcg gctacccatt tgtttgacgc
    36541 tccgcacctg agtaattaat acgccagact gcgctaccag gaagcgaccc ggttcaattt
    36601 ccagtttcac agggtggccc aaatggcggg cgatttgctc acgcgcggca ttccacagac
    36661 cataataatg ttcggtatca accgcctctt caccctgttg ataaggaaca gaaagcccac
    36721 cgcccgcaga gatagcctgt aaatcctgac cgaattcgat gacctgacgc accatagcac
    36781 cacacacctg ttccagatgg gcataatcaa cgccagaacc aatgtgcatg tgaatgccga
    36841 ccagctgcag atgatgacgt tgtatcacgt ccagtgcggc gggcagatcg gtgtaccaga
    36901 taccgtgctt gctgttttcg ccaccggtat tggttttttg gctatgtccg tgaccaaacc
    36961 ccggattaac gcgcagccat acccgatgcc ctggcgaaac caggcccagt tggtcgagca
    37021 tatcaacaga acccgcattc accggaattt gcaattcact gacgcgttca agcgtcgcct
    37081 gatcgataac atctgccgta aaaacaatat catcggggtg cgtttgcgga ttgtaacccg
    37141 ccgccaacgc acgctctatt tcgcctaacg agacggaatc cactttcacg ccctgctcac
    37201 gcattaagcg caaaatatga atattggaac aggctttctg tgcaaagcgc accacatcaa
    37261 actgtttcag cgctgcaatc tgccgacgaa taatttgcgc atcgtagacc cacaccgggc
    37321 agccaaattc agcgggcaaa cgcagcagat tttcggcggt gagatcggta tcggtgctga
    37381 acagtgaatg tggcataaca aactccagat aagtgctttt ttatgattac gccacatcat
    37441 aaaaagaata aaaaatatcg atttatgtcg agtctatgca aaaatgatat ggattaccgg
    37501 attgcgagag agcgctaatg gccgccgtta acttacgtca tattgaaatt tttcatgcgg
    37561 taatgaccgc cggaagcctg actgaggcag cacacctgct acacacctca cagccaaccg
    37621 tcagccgcga actggcgcgc tttgagaagg tgatcgggct aaaattgttt gagcgcgtac
    37681 gtggacgatt acatcctacc gtgcaaggac tgcgtctgtt tgaagaagtg caacgatcct
    37741 ggtacggact ggatcgcatt gtcagcgccg cagaaagtct gcgcgagttt cgccagggag
    37801 aactgtctat tgcctgcctg ccggtctttt cgcaatcttt tttaccgcag ctcctgcaac
    37861 cctttctggc acgttatccc gatgtcagct taaatatcgt gccccaggaa tcaccgctac
    37921 ttgaagagtg gctctcggcc cagcgtcatg atttaggact cactgagaca ctccatacgc
    37981 ctgcgggaac agaacgtacc gaattactct ctttagatga agtgtgtgtg ttacctccgg
    38041 gtcatccgct ggcggtaaaa aaggtattaa cgccggatga ttttcagggt gagaactaca
    38101 tcagcctttc ccgtactgac agctatcgcc agttgctgga tcagctattt actgaacatc
    38161 aggttaaacg acgcatgatc gtagaaaccc acagcgccgc gtcagtctgc gcaatggtac
    38221 gggcgggggt aggtgtttcg gtggttaacc cgctcaccgc actggattat gcggcaagcg
    38281 gtttagtggt gcggcggttc agtattgcgg ttccgttcac cgtcagcctg atccgccccc
    38341 tgcaccgccc gtcatcagcg ctggtgcagg cgtttagtga gcatttacaa gcggggttac
    38401 cgaaactggt cacttctctt gacgctattt tgtcgtcagc tacgacagca taaaagcgac
    38461 agcatcctcg gcatggatcg ccgcagtatc aaacacaggc aaaacactgc gctcttctgg
    38521 caccagtaaa ccaatttctg tgcagccaaa aatgacgccc tgtgcgccct gttctgcaag
    38581 gcgagcaatc acttgcgcat aataagcgcg tgacgcttcg gtaaattgcc ccagacacag
    38641 ttcttcaaaa ataatctgat taattttcgc ccgttcatcc gcttcaggaa taagacaatt
    38701 gatggaaaat tgttccgtca gccgcccgcg ataaaaatcc tgttccatgg tgtaacgcgt
    38761 acccagcagc gccacacgag tcattccggc cccggtaatt gcacgtccgg tggcatccgc
    38821 aatgtgtaag aaaggcagag tgcaacgtga ctcaatggca tccgccactt tatgcatcgt
    38881 attggtacat aacacaatac cttctgcgcc cgcccgctgt aagccaagcg ccgcctcagc
    38941 cagaatgtcc ccggttttat cccattcccc gcgacgctgg cactcatcta tttcatgaaa
    39001 atcgacgcta tgtagcagca cttgcgcaga gtgaagccca ccaagccgct gtttaatgcc
    39061 ttcatttatc agacgatagt aaggaatggt ggattcccag ctcattcctc ccagcaaacc
    39121 aattgttttc attcctctct cctgatgtgt gttaccgcaa caaggctgaa taaggaacga
    39181 gacaaacgcc tcaacggcca ggtgcccgat ctctattaac gaaaaaaggg ccggatgtac
    39241 agcacatccg gcccgtgaaa tcagacgccg atatttctca acttctcgcc tgccatcagt
    39301 ttgcgttcga tatgttccag cgtgacattt ttggtttccg gaatgagcca gaaagtgatg
    39361 cccacaaacg caatgttcag cgcagtgtag agccagaacg taccggcagc gccaatgcta
    39421 tcaagcagtg tcaggaaggt cgcgccgata atcatattcg acacccagtt tgtcgtcgtc
    39481 gaacaggtaa taccgaaatc gcggcatttc agcggctgaa tttcagagca caggatccac
    39541 accactggcg cggcgctcat cgcataaccg gcaatacaca tcatcgtcat gccaacagag
    39601 agccaggaca agccactgga agccgtaccg ttatcaaact gcatcaggca atagcccagc
    39661 accagagtgc ctaacgccat cacgctgaaa ccaattttca gagccggttt acgacctgct
    39721 ttatctaccg taaagaccgc aatgaaggtc gcgaacataa aggtcagtcc cacgaccaga
    39781 gtcgcaatca tctgttgttc tgtggtcgta aagcccgcca ttttgaagat acgcggcgca
    39841 tagtacatga tgatgttcat accggtaaac tgctgcatcg cctgcaacaa cataccgaga
    39901 aacacagcac gacggacgtt acggttgatc ttaaacagtg cccaaccgcc ttgttttaac
    39961 ttcaggcttt cacgaatttc gttgagttct tctcgcgctt tttccgacgt atcgcgcagc
    40021 atacgcaaca cttcttccgc ctcaatatga cgcccctttt ccgccagcca gcgcgggcta
    40081 tttggcagga agaccaccag aataatcagc aggactgctg gtaaagcaag aacccccaac
    40141 attgcgcgcc agttaccgct ataactgaac gctgtatcgg ataaaaacgc cagcacgatg
    40201 ccgagtgtga ccatcaactg gtacatactg atcatcttac cgcgaacgtt ttcacttgcc
    40261 atttcagaaa gatacagagg agcggtgtaa gacgcgatcc cgacagcaat gcccagcacc
    40321 acacgagcgg cgattaacat ctctacgctg gtcgcaaaag cggaccctat agaaccgagt
    40381 acaaacagga tggcccccgc catcaggctg tatttacgac ccaggcggaa cgacagccaa
    40441 ccattaaaca gcgcaccaat tgctgcgccg agcatcatgc tactaaccac ccattcctgc
    40501 aaacgactgg tcagcacaaa gtgatcggta atgaacggca acgctccggc gattacgccg
    40561 atatcaagac caaataacaa tcctgcgacc gcagcagcta ccgaaacaaa catattcata
    40621 cgccgcgtat cccgcaaaga acgtggcgtt aaagcagatt ccgtattgat agtaaccatt
    40681 ttttcctgcc agcagagagt aagacatagt gaaaaaatac gtgaacaact cacgcaggtg
    40741 tcaggtcgga aacagcataa atatggatta aattgctgcg acatgtcgtt atgtgatgga
    40801 tattccaatt ttcaaattaa gttgaattat tgagattatt attaaccacc taattttaca
    40861 gcagataaaa ttcataaagt tcattaattg ataattaata tggattattt cataaccatg
    40921 atatggatta tgatgatcta caggtataaa aaaccctgcc atgcggcagg gtcataaaag
    40981 taagaagaat gaattaacgc gccagccaac cgccatccac ggcaatggta taaccattca
    41041 cataatctga agcgctggag gcaaggaaca ctatcggccc catcaggtca ctcggcagtc
    41101 cccaacgacc agctggaatg cggtcgagaa tttccgcgct acgttgttca tctgcccgta
    41161 gttgttgagt attgttggtc gccatgtaac ccggagctat cgcattaaca ttaatgttgt
    41221 gtttagccca ttcgttcgcc atcaatcgcg tcacacccat cacgccgctt tttgatgcgg
    41281 tataagaagg cacacggatc ccaccctgga aggagagcat tgacgcgata ttgataatct
    41341 tgccgccatt gccttgcgcg ataaagtgtt tcgccgctgc ctgagacatg aagaatacgc
    41401 tcttgatatt caggttcatg acatcgtccc agtctttttc gctgaactcg agagcgtctt
    41461 cgcggcgaat caatccggcg ttattcacca ggatatcaat atgaccaaat tcagctaccg
    41521 cgcgatccag cagtccagga atgccatcaa tctttcgcag atcggcggtc aggcttaaaa
    41581 aacgacgccc cagcgccgtg acctgctcga tggtttcagt cggttcaacg atgttaatgc
    41641 caacaatgtc acagcccgct tgcgccagcc ccaacgccat cccctggccc agcccagtat
    41701 cacaaccagt gacgaccgca actttacctt cgagagaaaa tgcacttaaa atcataacaa
    41761 taccttattc gtttatgccc acaactagcg caaatcttta acggccacat ggtccatatc
    41821 atcaaagacc tggttttcac cgaccatgcc ccagataaag gtataagctt tggttccgac
    41881 gccggaatga atcgaccagc tcggggagat caccgcctgc tcgttatgca tcacaatgtg
    41941 acgcgtttct tgcggctgcc ccatcatgtg gaaaacgcag gcgtcatcat ccatattgaa
    42001 atagaaataa acttccatcc ggcgctcgtg agtgtgacac ggcatggtgt tccacaagtt
    42061 acctggagcc agctccgtca gccccatact caattggcag gtttccagca catccgggac
    42121 aaaatatttg ttaattgtgc gacggttact ggtgaggtta tcgcctaacg tgactggaga
    42181 tacttcgtcc ggtgtgactt ttttggtggg atacgtcgta tgcgcgggtg cgcaattgta
    42241 ataaaacttt gccggagtgg cggtatcaat actggcaaag acaacttctt ttgcaccttt
    42301 acccacatac agggcgtcgc ggtgaccgat ttcatagcat tggccatcga cagtaatcgt
    42361 accggcaccg ccaatattga taacacctaa ctcgcgacgt tcaaggaaat agcttacgcc
    42421 gagttgttta ccaacttccc cgccaacgga aaccgtttta gttatcggca taatgccgcc
    42481 aacaataatt cggtcaatgt ggctgtaaac catggtgtac tcatcggcga caaatacctc
    42541 ttcaaccaaa aattcattgc gcagcccttg ggtatccagc gtttttgcgt gcgcactgtg
    42601 gatgctctgt cttacgtcca catcaacctc cgataatcag tgaagcgtga aaatagaaaa
    42661 cgatagtgaa acactgtttc gttttctcta taaagggatg atatgcccat gggctagggt
    42721 tttcaattaa aaataaaaca atgtttcatt tttaagttaa ggattaaaaa aagtgcagtg
    42781 tcgatcacga atatgtcatg agaatggaaa aatgagggag cccagaaaat gtatgtcgac
    42841 ttgccagcca tcattattca acagattaat gacggcagat atttatcggt aaggaggaaa
    42901 tgcagagcta ctcgtcacgt tcaatggtca atgccacgcc ctggcccccg ccgatacaaa
    42961 gcgttgccag ccctttgcgg gcattacgtt tcaccatttc atgaaccaga gaaaccagga
    43021 ttcggcaacc ggaagcgcct atcgggtgac cgagtgcgat cgcgccacca ttgacattga
    43081 cccgacgctc atcccactca agcatcttgc caaccgaaag cgcctgtgca gcaaacgctt
    43141 cattagcctc gataagatcg acatcagcca actgccagcc tacacgctcc aggcaacggc
    43201 gggtcgcata caccggcgca attcccatca atgccggatc tacaccaacg ctggcaaatg
    43261 cgcggatgcg ggccagcacg ggtaaattca acgctcgtgc tttggcttcg ctcatcatca
    43321 ttaccgcagc tgcgccatcg tttatggatg atgcattccc cgcggtcaca gaaccgagac
    43381 tatcaaatga aggatttaaa cgggctaaac cttctgcact ggcgtcagtg cgtggctgtt
    43441 catcggtatc aacaaccaac gtctgaccgt tactttggat cattaccggg acgatctcat
    43501 ctttaaatcg tccggcgtca atcgccgctc gcgctttttg ttgcgagcta agtgcgtaag
    43561 catcctgcaa ctgacggctg atgccatatt cgcgagccag attttcggcg gtgacaccaa
    43621 tatgataatc attgaaggca tcccacaacc catcatgcac cagactgtca accaactggc
    43681 tattgccaag ctgtgcaccg gtgcggctat cagtcagaac atgtggtgcg cggctcatgt
    43741 tttcctggcc accggcgatg acaatatcag cctcgccaca ctgtatcgcc tgagtagcca
    43801 gatgcagtgc tttaagccca gaaccgcaaa cgtcattaat agtgattgca gaaacgctat
    43861 taggaagacc acctttaata gccgattgcc ttgccggatt ctgccctgcc cctgcagtca
    43921 acacctgacc aagaattact tcatccaccg catatgcagg aacgccggta cgttctatta
    43981 acgctttcac gaccagacta ccaagttcca cggcggaatg acccgctaac gcaccgcgaa
    44041 agcagccgat aggtgtccgt aacgccccga caatcacaac gtctttcatc acaacctcgc
    44101 accaattaac agtgcaatag taaatgattg ttaattacag ttatcttaat tgtttaaaaa
    44161 aagtgatttt tatcacaaag gaaatatgcc tgagcagtag tcagagtcat aactggcacg
    44221 taaggtttgc aaccactaac ccaccaatag aggggtagat agggcgttaa tctcccatac
    44281 ttaacctggt ttatggtaaa ttgccctcca ttttgtttaa tttgtagatg atacgttcag
    44341 ataatgtcta atatttggtc taaagaagaa actctgtgga gtttcgcgct ctacggcaca
    44401 gccgttggtg caggcacgct cttccttcct attcagttag gttcggcagg ggctgtggtc
    44461 ctgtttatta ctgctctggt cgcctggcct ttaacatatt ggccacataa agccttatgc
    44521 cagttcatcc tctcatcgaa aacatcagca ggtgaaggga taacgggcgc agtaacacac
    44581 tactatggca agaagattgg taatctgatt accacgctgt acttcatcgc cttttttgtc
    44641 gtcgtgttga tatatgcagt ggcaattacc aactcactta cggaacagct ggcaaagcat
    44701 atggttattg atcttcgcat ccgtatgttg gtgagtctgg gtgttgtatt aatcctgaat
    44761 ctcatttttc tgatgggacg ccatgcaacc attcgggtaa tgggtttttt ggtattccca
    44821 ttgattgcct atttcttatt tctttccatt taccttgtcg gtagttggca acctgatcta
    44881 ttaacgaccc aggtagagtt caatcagaat acccttcacc agatatggat atcgattccc
    44941 gtgatggttt tcgcctttag ccatacgccc attatttcta cgtttgccat agacagacgt
    45001 gaaaaatatg gcgaacacgc tatggataaa tgcaaaaaaa ttatgaaagt cgcttatctc
    45061 atcatctgca taagtgtact gttctttgtc tttagctgcc tgctttctat tccaccttcg
    45121 tatattgaag cggctaaaga agaaggggtc accattttat cagcgctttc tatgctgccg
    45181 aacgccccag catggctgtc aatttccggg attattgtcg cagtagttgc gatgtcgaaa
    45241 tcattcctgg gtacgtactt tggcgttatt gaaggtgcca cggaggtcgt caaaacaaca
    45301 ctacagcagg ttggtgtaaa gaaaagtcgt gcatttaacc gcgcactatc aattatgttg
    45361 gtatcgctga tcaccttcat tgtttgttgc attaacccga acgcgatttc gatgatttac
    45421 gcgatcagcg gcccgctcat tgccatgata cttttcatca tgcctacgtt gtcaacgtat
    45481 ctgatcccgg cgcttaaacc ctggcgttcc atcggaaatc tgattacgct gatcgtgggt
    45541 atcctgtgcg tatcggtaat gttctttagc tgatatccat tgtgaatggt tcaaaattgc
    45601 tacaattttg aaccattcca gttttcataa tgttcattcg ttattattcc gcactatcac
    45661 ttccctcatc atatcctagt attatcagtt agttaaacac acaaagagat ctgaacatct
    45721 taaataagac tatttaagat gcataactta gattcgcaag atatcttccg gtcattcaaa
    45781 aacaatttgc gatatagata ttctcatacc atgagatagt tatctgaacc accctcaacc
    45841 aggtaagatt aaatcagtga aacataatca tatttaataa cgagaaatgc attttaaaat
    45901 gtcttaatca gaattatagc aaatacagat taatcgcaat ttttcccagc aaaattacaa
    45961 aatcatggtg tattgacatc atcaatcacc aatgaaatta tgcaattata tacggatagg
    46021 gaggttctta acatggggct ttgtagtcgt tataaaagtc ttacatgcaa tagttgctca
    46081 atgcattgcc aaataatgcc agaagagtca ccgcgtttgc agtactgcgc aaactcgtgt
    46141 ttttgtatgt ggcccgaaga aagttcatat tttaatcgtg gtgtggtaga aggtatctta
    46201 acaaaaaacc acaatgcgag attaagcgga tatatttttg tcgatttttc agtaagtttt
    46261 ttacgtctat tccttgaaaa agactggatt gactatcttg caagtactga catggggatt
    46321 gttttagtca gcgaccgcaa tatgcaatcg ttagctaatt actggcgaaa acacaactca
    46381 gcaatatctg ctgtcatata taatgatgat ggtcttgatg ttgccaatga aaagatcaga
    46441 caactattta ttggtcgtta tttatcattt accagaggta acacattaac tcagatggaa
    46501 ttcaccataa tggggtatat ggtttctggt tataatccat atcagattgc tgaagttctg
    46561 gatatggata tccgtagcat ctacgcgtac aagcaacgaa tcgaaaagag aatgggtggt
    46621 aaaataaacg aattatttat tcgttcacat tcggtccaac attgatactc aacgaccagc
    46681 cagaatcata ctctgtttat acgggcattt attgaaatgt ccgtatttta taaataggcc
    46741 gtcccctcaa gagtaaacac catagtattt aaaaccatca cacgaaaaat tcagaagcat
    46801 tacgaaatat gaatttacaa acaatagtgg cataaatgtt aaccatgtta atttacgtaa
    46861 agttttacgt tgcaacatta aagcctcatt tcaatcatca tgataaatat aaaattaata
    46921 tatatttatg ccgtaaatac caatatactt agcaaactat gtgatctcca tttcgattga
    46981 tttagtgttt attgacgtat gtactggatt attaacgata atatcgagtt ctggcctgag
    47041 caccgaaaat taatatcggt acataacgcc gatcttaacg tcgttctgac aacgccagcc
    47101 agtcgatgtt tatcacttct acttgaagct tttcctgatg tggttgcaca acaagatttt
    47161 ttcaccagag tctgggaaga agaaggtatg cgtgtgccta ctaacacgtt atatcagaac
    47221 atatccatta tcagacgcgg atttcgcgct gttggtgata ctacccactc gctaattgca
    47281 accgtgccga gaagaggatt caagatccat aatgacacta acatacaaaa tcatgtaata
    47341 aactcgtcaa cagacgcaca tacacacaat gccccacctg ccataaaagt taatgcggga
    47401 tacaaagaga gcattggtgg cgcaaagaat tttaataaca aaatcctcaa acatataaaa
    47461 agtcatctaa ttatgttgag cgcatttgtc ataggcgcat attctgcata ttggctatgg
    47521 aataataatc aaccgaagcc atttttcaag gattacaaga ctgttgcgga aattaatggc
    47581 tgccatttta atgtaacaga agatacaatt gacgggttga aagagttcga taaatataaa
    47641 acacggatac tggattcggg gattaactgt aaaaaacatc cgtggttata cttccctctt
    47701 gcaaaatcct caccggggat gattgttatg gcatgcaata aaaattataa ccaacatgaa
    47761 gtggcagact gtttaaccct gtcttaccgt gaggttaatc gtgattgatt ataaaaaaaa
    47821 cttattattt attttagttt ttatcagtgg gtttattctc ttcactgtct attcttacac
    47881 tgcagaaaaa atgatatata acgaaacatg cactgcaaat tgggtaattt ttaatgacca
    47941 gggacgagca aatcttacca tcgactttat gtttaacaaa aaaaataaaa caggaactgt
    48001 tgcgttgagt ggaacctggc aacaaggtaa cagagaaagt aaatcaatac ggcgaaatat
    48061 tgaatacaca tggattgaaa actatgacac agcccattta acatcaaaga aagttaataa
    48121 gtttgagatt atggatcaag ttgatgatga tagacttgta caacttattc ctgattttta
    48181 tgtctttcca gaaaaaagtg taagctataa tatactaaag caaggtaagc atgcttttat
    48241 tttgagcatt ggtaacagag caataatgca ttgtgcaagg taaactaaaa tagataacaa
    48301 aaagatggaa cctcgttaat cataaaacaa taaaattaaa tattctcgca gtatatggca
    48361 gtctaaagca tcaaagtttt gatcaacatc tttcatttta gacatctcct tgcaatgtaa
    48421 aatatcatgt atcaacaaca tctggtgcaa atccatgagt cgaactcgac attcatctta
    48481 gctcgattat tattccttcg tacagtcgat gtaaacaata cagaaagagg attattaata
    48541 accagtttac tcagtgaatc aatagaggaa aggattaacg tttctttaaa agaattaatt
    48601 tcatcatctg ttaaactaaa ctcatcattg acagatcgtg agatataact gtttttaact
    48661 ttactcttta cgttgacttt attgacagaa ttaacattca catatcttga atttaatgtc
    48721 catgatgttg tttgcgtgag aaccttctca gcattaatga attttttaac ggaatccttg
    48781 gtttcttttt cgagtccgaa tttacaatat catgcatata gaccatttca tgaatctgcg
    48841 aaaactcaat gtccattgta tacctcacat ttttaccgtg actcgatgtt actgttcaat
    48901 aatcaccttc catcaatact aaaattaata cccctaatgt gccgataaca aatatagtca
    48961 ttctacgtaa cgtctccata aggtgatatt tgacattatc agaagctgcg aattcggatt
    49021 ttgctctaat caaatgagag aaatgtaaaa tgaagccacg aaatattaat aatagcctat
    49081 cactgcaacc attagttcct gatcaggaga acaaaaataa gaaaaatgaa gagaaatccg
    49141 ttaatccagt taaaatcaca atggggtctg gtttaaatta tattgaacaa gaatctcttg
    49201 gaggaaaata tctaatacat gatttgtcaa taaagatagc ggatatttct gaagagataa
    49261 ttcagcaagc aatattatct gctatg
//
LOCUS       DAZFXT010000020        47336 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.020, whole genome shotgun sequence.
ACCESSION   DAZFXT010000020 DAZFXT010000000
VERSION     DAZFXT010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47336
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.020"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            68..700
                     /gene="comR"
                     /locus_tag="ACHKW0_001284"
     CDS             68..700
                     /gene="comR"
                     /locus_tag="ACHKW0_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HFO7474150.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(762..1301)
                     /gene="ycfJ"
                     /locus_tag="ACHKW0_001285"
     CDS             complement(762..1301)
                     /gene="ycfJ"
                     /locus_tag="ACHKW0_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HFO7474151.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1511..2815)
                     /gene="ndh"
                     /locus_tag="ACHKW0_001286"
     CDS             complement(1511..2815)
                     /gene="ndh"
                     /locus_tag="ACHKW0_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HFO7474152.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADKGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(3215..3757)
                     /gene="ycfP"
                     /locus_tag="ACHKW0_001287"
     CDS             complement(3215..3757)
                     /gene="ycfP"
                     /locus_tag="ACHKW0_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase YcfP"
                     /protein_id="HFO7474153.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPNENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(3780..4805)
                     /gene="nagZ"
                     /locus_tag="ACHKW0_001288"
     CDS             complement(3780..4805)
                     /gene="nagZ"
                     /locus_tag="ACHKW0_001288"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HFO7474154.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFKRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(4816..5640)
                     /gene="thiK"
                     /locus_tag="ACHKW0_001289"
     CDS             complement(4816..5640)
                     /gene="thiK"
                     /locus_tag="ACHKW0_001289"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019165 - thiamine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HFO7474155.1"
                     /translation="MPFRSNNPIMRDELLSRFFPQFHPVTTFHSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPRFAFLRQYRALSQLPACIAPKPHLYLRDWMVVDYLPGEVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRRLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTDQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(5621..6262)
                     /gene="lpoB"
                     /locus_tag="ACHKW0_001290"
     CDS             complement(5621..6262)
                     /gene="lpoB"
                     /locus_tag="ACHKW0_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HFO7474156.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(6276..6653)
                     /gene="ycfL"
                     /locus_tag="ACHKW0_001291"
     CDS             complement(6276..6653)
                     /gene="ycfL"
                     /locus_tag="ACHKW0_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HFO7474157.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(6656..7015)
                     /gene="hinT"
                     /locus_tag="ACHKW0_001292"
     CDS             complement(6656..7015)
                     /gene="hinT"
                     /locus_tag="ACHKW0_001292"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HFO7474158.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            7349..9538
                     /gene="fhuE"
                     /locus_tag="ACHKW0_001293"
     CDS             7349..9538
                     /gene="fhuE"
                     /locus_tag="ACHKW0_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015344 - siderophore uptake transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HFO7474159.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(9598..11031)
                     /gene="ptsG"
                     /locus_tag="ACHKW0_001294"
     CDS             complement(9598..11031)
                     /gene="ptsG"
                     /locus_tag="ACHKW0_001294"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005355 - glucose transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:1904659 - glucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HFO7474160.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(11326..12123)
                     /gene="ycfH"
                     /locus_tag="ACHKW0_001295"
     CDS             complement(11326..12123)
                     /gene="ycfH"
                     /locus_tag="ACHKW0_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7474161.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(12134..13138)
                     /gene="holB"
                     /locus_tag="ACHKW0_001296"
     CDS             complement(12134..13138)
                     /gene="holB"
                     /locus_tag="ACHKW0_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HFO7474162.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(13135..13776)
                     /gene="tmk"
                     /locus_tag="ACHKW0_001297"
     CDS             complement(13135..13776)
                     /gene="tmk"
                     /locus_tag="ACHKW0_001297"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7474163.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(13766..14788)
                     /gene="yceG"
                     /locus_tag="ACHKW0_001298"
     CDS             complement(13766..14788)
                     /gene="yceG"
                     /locus_tag="ACHKW0_001298"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HFO7474164.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(14791..15600)
                     /gene="pabC"
                     /locus_tag="ACHKW0_001299"
     CDS             complement(14791..15600)
                     /gene="pabC"
                     /locus_tag="ACHKW0_001299"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HFO7474165.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(15720..16961)
                     /gene="fabF"
                     /locus_tag="ACHKW0_001300"
     CDS             complement(15720..16961)
                     /gene="fabF"
                     /locus_tag="ACHKW0_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HFO7474166.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(17049..17285)
                     /gene="acpP"
                     /locus_tag="ACHKW0_001301"
     CDS             complement(17049..17285)
                     /gene="acpP"
                     /locus_tag="ACHKW0_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7474167.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(17496..18230)
                     /gene="fabG"
                     /locus_tag="ACHKW0_001302"
     CDS             complement(17496..18230)
                     /gene="fabG"
                     /locus_tag="ACHKW0_001302"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HFO7474168.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(18243..19172)
                     /gene="fabD"
                     /locus_tag="ACHKW0_001303"
     CDS             complement(18243..19172)
                     /gene="fabD"
                     /locus_tag="ACHKW0_001303"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HFO7474169.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(19188..20141)
                     /gene="fabH"
                     /locus_tag="ACHKW0_001304"
     CDS             complement(19188..20141)
                     /gene="fabH"
                     /locus_tag="ACHKW0_001304"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HFO7474170.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(20209..21279)
                     /gene="plsX"
                     /locus_tag="ACHKW0_001305"
     CDS             complement(20209..21279)
                     /gene="plsX"
                     /locus_tag="ACHKW0_001305"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HFO7474171.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDVRPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(21360..21533)
                     /gene="rpmF"
                     /locus_tag="ACHKW0_001306"
     CDS             complement(21360..21533)
                     /gene="rpmF"
                     /locus_tag="ACHKW0_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HFO7474172.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(21585..22106)
                     /gene="yceD"
                     /locus_tag="ACHKW0_001307"
     CDS             complement(21585..22106)
                     /gene="yceD"
                     /locus_tag="ACHKW0_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HFO7474173.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            22305..22889
                     /gene="yceF"
                     /locus_tag="ACHKW0_001308"
     CDS             22305..22889
                     /gene="yceF"
                     /locus_tag="ACHKW0_001308"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HFO7474174.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(23001..23960)
                     /gene="rluC"
                     /locus_tag="ACHKW0_001309"
     CDS             complement(23001..23960)
                     /gene="rluC"
                     /locus_tag="ACHKW0_001309"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HFO7474175.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            24533..27718
                     /gene="rne"
                     /locus_tag="ACHKW0_001310"
     CDS             24533..27718
                     /gene="rne"
                     /locus_tag="ACHKW0_001310"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008995 - ribonuclease E activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HFO7474176.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVHVQPMVTEVPVAAAIEPVVSAPVVEEVAGVVEAPVQVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVVVAQEVAEQAEPVVEPQEETADIEEVVETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(27914..28867)
                     /gene="flgL"
                     /locus_tag="ACHKW0_001311"
     CDS             complement(27914..28867)
                     /gene="flgL"
                     /locus_tag="ACHKW0_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HFO7474177.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(28879..30522)
                     /gene="flgK"
                     /locus_tag="ACHKW0_001312"
     CDS             complement(28879..30522)
                     /gene="flgK"
                     /locus_tag="ACHKW0_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009424 - bacterial-type flagellum hook
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HFO7474178.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNSQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTATPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(30588..31529)
                     /gene="flgJ"
                     /locus_tag="ACHKW0_001313"
     CDS             complement(30588..31529)
                     /gene="flgJ"
                     /locus_tag="ACHKW0_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HFO7474179.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKVGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(31529..32626)
                     /gene="flgI"
                     /locus_tag="ACHKW0_001314"
     CDS             complement(31529..32626)
                     /gene="flgI"
                     /locus_tag="ACHKW0_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HFO7474180.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(32638..33336)
                     /gene="flgH"
                     /locus_tag="ACHKW0_001315"
     CDS             complement(32638..33336)
                     /gene="flgH"
                     /locus_tag="ACHKW0_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009427 - bacterial-type flagellum basal
                     body, distal rod, L ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HFO7474181.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(33389..34171)
                     /gene="flgG"
                     /locus_tag="ACHKW0_001316"
     CDS             complement(33389..34171)
                     /gene="flgG"
                     /locus_tag="ACHKW0_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009426 - bacterial-type flagellum basal
                     body, distal rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HFO7474182.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(34309..35064)
                     /gene="flgF"
                     /locus_tag="ACHKW0_001317"
     CDS             complement(34309..35064)
                     /gene="flgF"
                     /locus_tag="ACHKW0_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HFO7474183.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(35084..36289)
                     /gene="flgE"
                     /locus_tag="ACHKW0_001318"
     CDS             complement(35084..36289)
                     /gene="flgE"
                     /locus_tag="ACHKW0_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HFO7474184.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(36314..37009)
                     /gene="flgD"
                     /locus_tag="ACHKW0_001319"
     CDS             complement(36314..37009)
                     /gene="flgD"
                     /locus_tag="ACHKW0_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HFO7474185.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     HGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(37021..37425)
                     /gene="flgC"
                     /locus_tag="ACHKW0_001320"
     CDS             complement(37021..37425)
                     /gene="flgC"
                     /locus_tag="ACHKW0_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HFO7474186.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVGDVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(37429..37845)
                     /gene="flgB"
                     /locus_tag="ACHKW0_001321"
     CDS             complement(37429..37845)
                     /gene="flgB"
                     /locus_tag="ACHKW0_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030694 - bacterial-type flagellum basal
                     body, rod [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HFO7474187.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            38000..38659
                     /gene="flgA"
                     /locus_tag="ACHKW0_001322"
     CDS             38000..38659
                     /gene="flgA"
                     /locus_tag="ACHKW0_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009428 - bacterial-type flagellum basal
                     body, distal rod, P ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation chaperone
                     FlgA"
                     /protein_id="HFO7474188.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            38735..39028
                     /gene="flgM"
                     /locus_tag="ACHKW0_001323"
     CDS             38735..39028
                     /gene="flgM"
                     /locus_tag="ACHKW0_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis anti-sigma factor FlgM"
                     /protein_id="HFO7474189.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            39033..39449
                     /gene="flgN"
                     /locus_tag="ACHKW0_001324"
     CDS             39033..39449
                     /gene="flgN"
                     /locus_tag="ACHKW0_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HFO7474190.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTIHRGGKKISI"
     gene            complement(39489..41024)
                     /gene="murJ"
                     /locus_tag="ACHKW0_001325"
     CDS             complement(39489..41024)
                     /gene="murJ"
                     /locus_tag="ACHKW0_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HFO7474191.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPALLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(41134..42057)
                     /gene="yceM"
                     /locus_tag="ACHKW0_001326"
     CDS             complement(41134..42057)
                     /gene="yceM"
                     /locus_tag="ACHKW0_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7474192.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(42059..42706)
                     /gene="yceH"
                     /locus_tag="ACHKW0_001327"
     CDS             complement(42059..42706)
                     /gene="yceH"
                     /locus_tag="ACHKW0_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HFO7474193.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSCAARMYEFSDMAEVESTLEQLANREDGPFVVRLARELGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(42717..43301)
                     /gene="rimJ"
                     /locus_tag="ACHKW0_001328"
     CDS             complement(42717..43301)
                     /gene="rimJ"
                     /locus_tag="ACHKW0_001328"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7474194.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            43537..44745
                     /gene="mdtH"
                     /locus_tag="ACHKW0_001329"
     CDS             43537..44745
                     /gene="mdtH"
                     /locus_tag="ACHKW0_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HFO7474195.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVHTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            44809..45456
                     /gene="grxB"
                     /locus_tag="ACHKW0_001330"
     CDS             44809..45456
                     /gene="grxB"
                     /locus_tag="ACHKW0_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HFO7474196.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNLADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            45590..46150
                     /gene="yceB"
                     /locus_tag="ACHKW0_001331"
     CDS             45590..46150
                     /gene="yceB"
                     /locus_tag="ACHKW0_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7474197.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            46256..47302
                     /gene="pyrC"
                     /locus_tag="ACHKW0_001332"
     CDS             46256..47302
                     /gene="pyrC"
                     /locus_tag="ACHKW0_001332"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0019856 - pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HFO7474198.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
BASE COUNT        11441 a        12823 c        11783 g        11289 t
ORIGIN      
        1 actggtgaac ggaggtgatt atttttataa taacctttca ttcacagtca ccaggtacaa
       61 cggcatcatg gcaactgact caacacaatg tgtaaaaaaa agccgtggcc gcccaaaagt
      121 gttcgacagg gatgccgcgc ttgataaggc catgaaattg ttctggcaac acggttatga
      181 agcgacttct cttgcggacc tcgtcgaagc gaccggagcc aaagcgccca cgctatacgc
      241 ggaatttacc aacaaagagg ggttatttcg tgccgttctc gaccgctata tcgatcgttt
      301 tgccgctaag catgaagcac agctgttttg tgaagagaaa agcgtggagt ctgcgctggc
      361 tgactatttt gctgccatcg ccaactgctt taccagcaaa gacaccccgg caggctgctt
      421 catgatcaac aactgcacca ccctctcccc agattcagga gatatcgcca atacgttgaa
      481 atcacgccat gcgatgcaag agcgcacttt gcagcagttt ttatgtcaac gacaagcgcg
      541 cggggaaatc ccgacccact gtgacgtgac acatctggca gaattcctta attgtattat
      601 tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg gaaaaactaa tgcagattgc
      661 cggaacgact ttgcgtttat ggcccgaact ggtgaaataa gtcgcaaaaa aagcccctca
      721 gcctgagcga atgaggggcc aaattgcaga gtacagcctt gttatacttt gttattcaaa
      781 atcagttgcc cattactatc tagcgggatc tgcgtacccg gatcgcggtc catgcggatt
      841 ttgccctgct gatcgccaat cttataggtc acatcataac cgagcatttt ttctgacttg
      901 tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag tatcgctttc ctggagagag
      961 ccctggatct ggttaccggc atatccacca cccagcgccc ccacaacagt ggcgacatct
     1021 ttaccgcgac caccgccaaa ctgatgccct atcacgccgc cagcaacagc gccgagcacc
     1081 gacccggtaa tgcgattttc atcctgcacc ggtcgacgat gggtcactgt gacgttgcga
     1141 cactcctgac gcggtgtttt aaccgtttcc ttgattgggg ttgcagaaac aacctgagcg
     1201 tattgcgggc cacgttcaaa cacgttcaga ctggccactg ccgctacgcc cagcgcagct
     1261 gcgacaccaa tcccgatacc cgccaacatt gatttattca cgggaacacc tccttctttg
     1321 cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta tcagacagaa gatgaaaagc
     1381 gcgggaatgg ctggaaatac ttaaggattc ggaggagtct gaaggaatgc ctgatgcgac
     1441 gcggctgccg atcgcgctgt tcctcaaggg cggataaggc gtttatgcca catccgccag
     1501 tgtacgtcga ttaatgcaac ttcaaacgcg gacggataac gcggttaata ctccccacca
     1561 gcatcattaa tccggtttta aagtaaccat gcagcgcaat ctgatgcatt cggtatagcg
     1621 agatatatac aaagcgcgca attcgtcctt caatcatcat tgagccgcgc gtcaggttac
     1681 ccatcaggct accaacggtg gagaagttcg acagcgatac cagcgaacca tgatctttat
     1741 actgataatt tttcagcggc ttaccattca tctgcgccag aatgttgttc attgcgcaag
     1801 tcgccatctg gtgtgcagcc tgagcacgcg gcggaacaaa gcccccttcc ggacgcgggc
     1861 atgacgcgca gtcgccaata gcgtaaatgt ctggatcgcg ggtggtttgc agcgtcggtt
     1921 ccaccaccag ctggttgata cggttagttt caagaccacc gatatctttc aggaagtctg
     1981 gcgctttgat cccggctgcc cacaccatca gatcagcctc aatatattcg ccatctttag
     2041 tgtgcaggcc gcctttatca gcactggtga ccatggtttg cgtcagcacg cgaacgccaa
     2101 gtttcgttag ctcgttgtgg gccgcagcag agatacgtgg cggtaatgca ggcaaaatac
     2161 gttctcccgc ttctaccagc gttacgttca gggcttcgtt ggtcaggcct ttgtaaccgt
     2221 agctgtgcag ttgcttgacc gcgttgtgca attcagcgga gagttctaca cccgtcgcgc
     2281 cgccgccgac aatcgcaatg ttcactttgc catttgcgcc caggttggcg gagtatttca
     2341 ggaagagatt cagcatctcc tggtgaaagc gacgcgcctg gtgcgggtta tcgaggaaaa
     2401 tgcagttctc tttgacacct ggcgtattga aatcgttaga ggtgctaccc agcgccatta
     2461 ccagggtgtc ataggcgatt ttacgttccg gaactagcag ttcacctttc tcatcgcgca
     2521 gttctgcaat agtgattgtt ttcgcttcac gatcaatatc aatgacggaa cccagctgga
     2581 actggaaacc atgattgcgc gcatgggcca gatagctcaa cgcatcgacg ccttcatcaa
     2641 gcgagccagt cgccacttcg tgcagcagcg gtttccacag atggctgtgg ttacgatcga
     2701 ccagcgtaat tttggctttt ttcttgcgtc ccagcttatg ccccagctgt gttgccattt
     2761 ccagcccacc agcaccgccg ccgacaatca caatcttttt caatggcgta gtcaacgtga
     2821 cccccttaaa tttattaacc aattgttaat taatggttat taacatagct cttaattaac
     2881 aacaggttac agcaatgaaa atgttcttcg ctattgagaa taacatgaat ggtgcattgg
     2941 tcataccaaa attgatgtta atcaagtttt gttgctgaaa agataggcga ctttaccggg
     3001 tgagatcaaa aaacagagtt tattgtttat tatttgagca agcccggaag ggcgatatag
     3061 ggtgaagcgt atatcgaggg cgtttggtcg tcctgataag aggcgcatca ggcattgatg
     3121 ctcgaatgtc ggatgcggcg tgaacgcctt atccgaccca cagaatccgg agcgcatcag
     3181 gtaccggcaa ggttacctga tgcgacgagg gcatttaccc gagggttttg aacgctttaa
     3241 tgcgctgtaa atgcggggag atattcttga atttgtgcgt ctgctcttcg tcccagacaa
     3301 tctcgtaata atgatgcagc tcttcagatg tccgctggct gttaagcgct tcatcattac
     3361 gcgacaaaat caccaggcaa cgatcgcgat tcttctcacg gaagttggtc acacacttag
     3421 tggcaatatc ggcatactct tccgggcgat caatcttgcc ttccatgttc tcattaggga
     3481 acaaattagg gttgaagatc acctggcgga tatcgcaaag aaaaccaatc cgttccgccc
     3541 agtatccgcc cagaccaacg ccgcaaatca gcggacgctc gtcgacgttc agttgcaaca
     3601 ttttgtccac ttctttgagc agatgctgca tatcatgttt cggatgccgc gtactgtagc
     3661 ttatcaagcg tacatccggg tcaataaact gcaattgtaa gactttctcg tggttacccg
     3721 gactgttaga gtcaaaaccg tgtaaataga taatcatcgc ttcctcacat aagccagggt
     3781 tagtgacctg ctttctcttc ctgccagcgt tcatgtaact gattcagacg ggtgctgatc
     3841 gctttccagc gagccgagtc catcagttcc tgtcgcgaaa atgaaccttt atgatacaaa
     3901 cgtgtaacac gttctgcctt gatcggtgac agattatcta acacgctgac ggccccttta
     3961 cgattattgc agaccaggat catatcgcaa cccgcatcca gtgatgcctg cccgcgttcg
     4021 gcataactgc ccataatcgc ggcaccttcc atcgataaat cgtcagagaa aatcacgccg
     4081 tcaaaaccca gttcctgacg caaaacggtt ttcagccagt agggagaacc gctcgccgga
     4141 cgcggatcaa catcactgta gatcacatgc gcaggcataa tggcgtcgag tttattttcg
     4201 cggattaagg aactgaagac cgacatatct ttagcgcgaa tctccgcttg tggacgtgga
     4261 tcgcacggtg tttctttgtg tgagtctgcc gttactgcac cgtgtcctgg gaagtgtttc
     4321 ccggtcgttt tcattccggc ttcatgcata ccatcaataa accggctggc aattgccagg
     4381 gctttttgtg gatcggcatg ataagaacgc tcgccaatcg ccgcgctgat atgcccgaca
     4441 tccagcacag gcgcaaagct gatatcaata tccatagcga tcatttcgct ggccatcagc
     4501 caacccgcct cttgcgccag tttgccgccc tcttccattc ccaacagcgc agcaaaggat
     4561 tgtgctgccg gtaagcgggt aaaaccttcg cgaaaacgct gcacgcgtcc accttcctga
     4621 tctaccgcca ccaccagatg attgcgcgaa gctgcgcgga tctggcgcac cagttcacgc
     4681 aactgggcag gatcatgata gttacgctta aagagaatca gccctcccac cagcggatgc
     4741 gcaagtattt cacgctcttc cgcgtccagt tcgtaacctt cgacatccaa cattactgga
     4801 cccacactgc tctccttatt gttttattaa tagctgccgc caggtgtcat cggccagcct
     4861 gataaattgt tgatcgccgg tttgtcgcca gcggtactca aaccaccctg ctttgagcat
     4921 cagcagccag ggaaaccatc gcctgacctg acgccataat tgcgccggat aaatcttcgc
     4981 gcgagtggca tagtcattga ccaattgccg gtgctggtca gtattttcca cccacaccgc
     5041 cgccagttcc agcgcgatat caccatctcc ggcatactcc cagtcgatga gttttaaccc
     5101 tgacgcgcta tgcactaaat ttccggcgtg gacatccata tgcaatggac ttaagcgtaa
     5161 aggccgtggt tcccgcgctt tgcgcagacg ttttaacctt cgcagccaac ccactgtccg
     5221 ccgcgccgga tcgctttgct gccagtacag ttccagtaac ggcaacagcg ttattcgcca
     5281 gccaaaacgc ggttgttgat gtagataata cagcaagcct gccagttcgt tggtatccgg
     5341 caaatacgtt tttacctcgc cgggcagata gtcgaccacc atccagtcac ggagatataa
     5401 atgcggcttc ggtgcaatac atgcgggtaa ttgtgataaa gcccgatact ggcgcaagaa
     5461 cgcgaaccgc ggcgcatcag gatcgtgcgg ctgacgtaca acaaaacgct ggccctggtg
     5521 ttcaatgaga aaactcccgc cactaagccc actatgaaac gtcgtgacgg gatgaaactg
     5581 cgggaaaaag cgcgacagca attcgtcgcg catgatggga ttattgctgc gaaacggcac
     5641 ctttacctga ccagataatt tcgcccgtct gcaccagcat cagctgcatt tgtagggtcg
     5701 gagcgttaac gttgccagag gcgctggagt acagcacgta atgagcgccg acattgcggg
     5761 caatgcctat ggctttacta cgagtaccta aactgtcctg cggcgacaaa cctaactgtt
     5821 gcttagccat cgacagctgc tgggcggaaa ccagggtaaa tttcccgtta ttagccagcg
     5881 catttcgcag cgtttcggtc gcttctgcgg cattcagcga accgttagta cggttgttaa
     5941 cgctatcaac cagcaggacg ctacccgcag tcaccccgtc agccccaagc atcttactga
     6001 ccatcggctg cattgcgcca ttccagtcat aatggcgaat atgcggcgca ggcggtgccg
     6061 tttgatcttc gtgctcaatt gggcctggct gctgcgggat cgtcggcacc gagggcactg
     6121 tggggacagg ctgttgtggc tcggctggtt gttccggcgc tggtttcact tcttctaccg
     6181 gtgcaggttc acgttgcccc acacacccgg cgagaaacat cgccagcgcg gtaatcaagg
     6241 cgtagcgact catttttgtc atcaagattc accccttaca aatatagata aagtctgact
     6301 ttgtgcgccc ccagaaaatt ggcgctgccg tacagcgtta ccgccgaatg tgcgggaatg
     6361 ctaacgctgc gtggcctttc cagaggatgc atctccagcc ctctggcgtc ataccagtaa
     6421 aaacgataat gaacggtaac gggttcttgc ctttcgttat aaagcgttga ggatgctgaa
     6481 ggttgaatat cagacgtcga aaggacgggc ttttctgcac tgatgcccgc agccaataaa
     6541 gatgactcca tcaccagcga ttgctcatca ttcaccggaa tttccggatg tgaacgacag
     6601 cccaccagca gtaacaacgc cagagacacc agcccaaagc atccttttct catcgttaca
     6661 gacctttatg cgccagcatt ggtcccagcg gacggccacc caacaagtgc atatggatgt
     6721 ggtaaacctc ttgtccgcca tggcggttgg tattcatgat cagacgatag ccatcttcgg
     6781 caataccttc ttgctcagca atttttgccg ctacggtgat catgcgtccc agcgcctgct
     6841 catgctcagc tgagacgtcg ttcacagtcg gaatgaggat attcggaatg atcagaatat
     6901 gcgttggcgc ttgcggcgaa atatcgcgaa acgccgttac cagatcatcc tggtagacga
     6961 tatcggaggg gatctcacga cgaataattt tgctgaatat agtttcttct gccacgacat
     7021 tttccttttt caaatagcca tgcgttgcgc catgctacgc cgcgccacac tgcgagtata
     7081 gagtatggtc gagttaaacc tgctctttca accttaactc acgatttctt aagcaaaaaa
     7141 tcgttttgct gccgtatata tcgccattat tcccatttct gcctgcgaat gcgctgaaaa
     7201 gacattctct gtttggttta cacgacaaat atgaatgcgt atatttctca tttgcattta
     7261 caaacaaaat tattcgcaca ctaaatataa tcgctcatca ccctcctccg gcatgagcct
     7321 gacgacaaca taaaccaagg gatttcaaat gctttcaaca caatttaaca gggataatca
     7381 atatcaagcc atcaccaaac cgtcactact tgccggttgc atagcactgg cactattacc
     7441 ttctgccgct tttgctgcac cagccactga agaaacggtg attgttgagg gttcagccac
     7501 agctccagat gatggcgaaa atgattacag cgtaacgtct acctctgcgg gtaccaaaat
     7561 gcagatgact caacgtgata ttcctcagtc ggtcactatt gttagccagc agcggatgga
     7621 agatcagcag ttacaaacgc tgggcgaagt gatggaaaac acgctgggga tcagcaaaag
     7681 tcaggcggat tccgatcgtg ctctttatta ttcccgcgga ttccagatcg ataactatat
     7741 ggttgatggt atccccacct attttgaatc gcgctggaat ctgggcgacg cactttctga
     7801 tatggcactc tttgaacgcg tagaagtagt gcgtggcgcg acaggactca tgaccgggac
     7861 gggtaatcca tctgcggcaa ttaatatggt tcgaaaacac gcgaccagtc gtgaatttaa
     7921 aggcgatgtc tcggcggaat acggtagctg gaacaaagaa cggtatgtgg cggatttaca
     7981 aagcccactc accgaagacg gtaaaatccg cgcgcgaatt gtcggcggct accagaataa
     8041 cgactcatgg ctggaccgct acaacagtga aaagaccttc ttctccggca ttgtcgatgc
     8101 tgatttaggc gatcttacga cgctttcagc cggttacgaa tatcagcgca ttgatgttaa
     8161 tagcccaacc tggggcggtt taccgcgctg gaatactgat ggcagcagca acagttacga
     8221 tcgcgcacgc agtaccgcac ctgactgggc gtacaacgat aaagagatca acaaggtctt
     8281 tatgaccctg aagcagcggt ttgctgatac ctggcaagcg acgctgaatg ccacccactc
     8341 tgaagtcgaa tttgacagca aaatgatgta tgtcgatgcc tatgtaaaca aagcggatgg
     8401 tatgctggtt gggccataca gtaattatgg acctggcttt gattatgtcg gcggcaccgg
     8461 ttggaacagt ggcaaacgta aagttgatgc gctggatttg ttcgctgacg gtagttatga
     8521 attgtttggt cgtcagcaca atctaatgtt tggtggcagt tacagcaaac aaaacaatcg
     8581 ttacttcagt tcatgggcca acatcttccc ggatgaaatt ggcagtttct acaactttaa
     8641 tggcaatttc ccacaaaccg actggtcacc acagagcctg gcgcaggacg ataccacaca
     8701 tatgaaatcg ttatatgctg ccactcgtgt cacccttgcc gatccgctgc atctgatcct
     8761 cggcgcacgt tataccaact ggcgggttga tacgctgact tacagcatgg agaaaaacca
     8821 caccacgcct tacgctggtc tggtgtttga catcaatgac aactggtcga cctacgccag
     8881 ctatacctct attttccagc cgcaaaatga tcgtgacagt tcaggcaaat atctggctcc
     8941 aatcaccggt aacaactacg agctgggtct gaaatcggac tggatgaata gccgtctgac
     9001 caccacgtta gccatcttcc gtattgagca ggataatgtc gctcagtcca ccggtacacc
     9061 tatccccggc agcaacggcg aaaccgccta taaagcagtg gatgggacag tcagtaaagg
     9121 ggtggaattt gaactcaacg gcgcaattac cgacaactgg cagctgacat ttggcgcaac
     9181 gcgctatatt gcagaggata acgaaggaaa cgccgttaat cctaatctgc cacgcaccac
     9241 ggttaaaatg ttcaccagct atcggttgcc tgtcatgcca gagttgacag tcggcggtgg
     9301 tgttaactgg caaaatcgcg tgtataccga caccgtgaca ccgtatggca ccttccgcgc
     9361 cgagcaaggt agctacgcgc tggtggatct cttcacccgc taccaggtga cgaaaaactt
     9421 ctcgttacag gggaacgtca ataacctgtt cgacaaaacc tacgatacca acgtggaagg
     9481 ttctatcgtc tacggcgcac cgcgtaattt cagcattacc ggcacgtatc aattctgaat
     9541 aacacctgta aaaaaggcag ccatctggct gccttagtct ccccaacgtc ttacagatta
     9601 gtggttacgg atgtactcat ccatctcggt tttcaggttg tcggatttag taccgaaaat
     9661 cgcctgaaca ccagaaccag caaccactac gcccgctgcg cccagtttct tcaggccagc
     9721 ctgatccact ttagacacat cagcaacgct gacgcgcaga cgggtaatac atgcgtcgag
     9781 gttagtaatg ttttctttac caccaaatgc agcaaccaga gccggtgcca tttcgctggt
     9841 acctgtcgct tttgcatctt cagtcgcgtc ttcacgaccc ggcgttttca gatccagtgc
     9901 tttaatcagc acgcggaaga tggtgtagta aacaatcgca taaccgatac cgacgatcgg
     9961 gaacagccac agtttgctgc tgttaccaga cagaacgatg aagtcgatca gaccgtgcga
    10021 gaacgacgta ccgtcacgca tccccagaag aatacagatt gggaatgcca ggcctgccag
    10081 aatcgcgtgg atgatgtaca ggatcggcgc aacgaacatg aaggagaact cgatcggctc
    10141 ggtgataccg gtcaggaacg aggtcagcgc cgcggagatc ataataccgc ccactttcgc
    10201 gcggttttct ggtttagcag agtgccagat agcaattgcg gcagctggca gaccgtacat
    10261 tttgaacagg aagccaccag acagtttacc cgcagtcggg tcacccgcca tataacgcgg
    10321 aatgtcgccg tggaaaacct gacctgctgc gttggtgtat tcaccaatct gcatctggaa
    10381 aggtacgttc cagatgtggt gcagaccaaa cggtaccagg caacgttcga tgaaaccgta
    10441 aatgccaaac gcaactaccg ggttctggta agcagcccac tgagagaagg tctggattgc
    10501 agaaccaatc ggcggccaaa tgaaggacag cacaacgcca gtaaagatgg cagccaggcc
    10561 agaaatgatc ggcacaaagc gtttaccggc aaagaagcca agatactcag gcagcttaat
    10621 acggtagaaa cggttaaaca tgtacgctgc gatcgcaccg gagataatcc ctccgagtac
    10681 gccagtatcc gccaggtgtt tagaggcgat ttcttcagca ggtaaatgca gtaccagtgg
    10741 cgcaaccacg gccatggttt taaccatgat gccataggca acaactgcgg ccagcgcgga
    10801 tacgccatcg ttattggtaa agccgagggc gacaccgatc gcaaaaatca gtggcatgtt
    10861 tgcaaagacg gaaccgcctg cttctgccat aacatgcgat acaacggcgg gcagccagct
    10921 gaaattcgcg gaaccgacgc ccagcagaat acctgcgata ggcagtacgg ataccggcag
    10981 catcagcgat ttaccgacct tttgcaggtt agcaaatgca ttcttaaaca taattgagag
    11041 tgctcctgag tatgggtgct ttttttacgt tctcacgcgt ggcaaggggg gagagcctcg
    11101 ccgtgtacag ggcatctaag cgccctttat ttattacaca gagtaaaata attcagtgcc
    11161 aatttgtttg acggctatca cgtttcaact tcagatcatt ggagaagttg cacattttga
    11221 caaaacatat gtcacaatat ttcaaacgtg gttattcacc gcccaatttt cggcggtgaa
    11281 ctttactcgt tttagccatt aattacgagc tttaaaaaaa ctcattcaac ggatggattg
    11341 aaggcgggaa gcgtcgatgt gaaacagacg ggcgaagtta tcggtggtta cctgcgccag
    11401 ttcttcaacg gcaacacctt tcaacacagc catgtattct gcaacgtcac gaaccatcgc
    11461 aggttgattc tcttttcctc gatgcggtac cggcgcaagg taaggtgagt cagtttccac
    11521 cagtaatcga tccaggggga cataacgcgc agcatcgcgc agttgctccg cattacggaa
    11581 ggtcacaatg ccggaaaagg agatgtaaaa tccgagatcc agtaatttac ccgccgtttc
    11641 tctgtcctct gtaaaacagt gtagtacgcc accgcaatcc gtcacttttt cttcgcgcag
    11701 aattgccagc gtatcggcac gggcgtcacg ggtatggacg ataaccggct tgttcagttc
    11761 acgaccaatc tggatatgat ggatgaagga ctcttgctga cgtactttag tttccggtgt
    11821 gtaataataa tccagcccgg tttcacccag cgctacaaca ccctcttctg ccgccagacg
    11881 gcgtaaatct tctacatcgt agggatcatt ctggttgagt ggatgcacgc cacaagaaaa
    11941 tacgacgttg tcacgttcgc ctaccagatc ccgcatatgt aaataacccg gtaatgttgt
    12001 ggcgactgcc agacaaaatt tcacatcgcg tgcggcggct ttcgccagaa cgtcatccac
    12061 gtccttatgc aaagattcat aatccagacc atcgagatgg cagtgtgagt cgactaaaaa
    12121 catgatgtct ctcttaaaga tgaggaaccg gtagcacaac gcccggttgc aggtaatgct
    12181 caatacgcag taaaagatcg gtgatgagaa gctcgcggtt gatgcctgta acagacatta
    12241 actgttcacg aatgtggcaa acatccccca gtatagcctg caggcgcgag ggagaaagat
    12301 ggtttgccag ttcggcgacc aggcccggca catcaacatt ggtcacctgc gcagcaccat
    12361 gatggcgttt tagcgcatcc atcagcaacg ttgccagcca gtgtaaacgc gccggagctt
    12421 gttcatgatt aagggccgct agcagcgaat accagtcgcc cgatggcacg ctatatgcca
    12481 acgcctgaca caatgtttca cgagcctgcc agttatctcc ctgaaacaac gccagtgccg
    12541 cgccaggcga accggcgctt aagcgcaatg cggcaagtaa tgcatcctgt gacattgtca
    12601 cttcgcgtga aagccaggtc acggcgtact gttccggcgg cggcgcaagg taatgtaacc
    12661 gacaacgact acgtaatgtt gccagtaaac gttcaggctc gcgggtagcc aggaaaaacc
    12721 aggtttctgc tggtggctct tcaagcgttt tcagcaatgc gttagccgcg gcgtcggtta
    12781 gtaaggcagc atcggttacc cagacaactt tcgcaccacc taagcgtgcg tgctcattca
    12841 gcttttcggt gacctcacgt accgcatcaa tgcccaacgc atttttccct ttttcgggag
    12901 ccagggtgta gtaatcggga tgcgtgccag cctgcatcaa ctgacatcca cgacagtgac
    12961 cgcaactttt gtggccctgc ggttgttggc agagtaaata acggctcagg gcgtagatta
    13021 aagcatcatc gcccatgccc ggtaacgcct gaatgagtag cgcatggtga cctcttccgg
    13081 cctgatagct ggctaccagt ttttcgaaat caggtcgtaa ccatggatac catctcatgc
    13141 gtccaactcc ttaacccagt gggtcacggt agtgcggatt gcatccatca cggcctccag
    13201 cggctgggtg gcatcaatgg tatgaatact tttatcttgt gcagccagtt ccagatagcg
    13261 ggcgcgggtg cgattaaaga aatcgaaaga ctcttgctca atacgatcca gctcgccgcg
    13321 cgcacgcgcg cgttttaagc caacttccgg ggtaacatcg agatagagcg ttaagtcggg
    13381 gcgaaaatcc ccgagaacag catcacgcag tgttgccagc atatgttggt caataccacg
    13441 tcctccgccc tgatacgcct gagtggagag atcgtggcga tcgccaatca cccaggtgcc
    13501 gttagccaga gctggtttga tgaccgtttc taccagttga acgcgcgcgg cataaaacat
    13561 cagaacttcg gctttatcgg taatgacttc atcgcctacc gatttgatat ccagcaccag
    13621 gcttcttaac ttttcggcaa gttgcgtacc gccaggttcc cgagtgaaaa ccatgtcgcg
    13681 gatacccagt tgctcaagcg tctcaaccac cacattacgc gcggtagttt tgcctgcgcc
    13741 ttccagcccc tcaatgacga tatacttact gcgcattttt ttccttaagc actttcagat
    13801 aatcctgcac agacttgtta tggctggcaa gattggtatt aaacgtgtga ccacctttac
    13861 catcggccac aaaatagaga tacggcgttt ttgccggatg cgcagcagcc ttcagcgaat
    13921 ccgcccccgg cgtagctatc gcacccggcg gcaaaccggt aatggtatag gtgttatacg
    13981 ctgtcggcgt ttccaggtct gcacgagaaa gtttgccatt ataacgctct cccatcccgt
    14041 aaatcacggt tgggtcggtc tgcaggcgca taccaatgcg taaacggttg ataaataccg
    14101 aggcaacctg atcgcgttca ctggcaacgg cggtttcttt ttcgataatt gatgccatcg
    14161 tcaccagctg atttttatct ttataaggca gaccgtccgc acgcccttcc caggcgctat
    14221 cgaccgcttt caccattttc ttgtgcgctc gcttgagtaa cgcgacatcg gtggtattgg
    14281 cggtatacat ccaggtgtct ggccagaacc aaccttcaat ccactccggg ttttccagtt
    14341 caagtgcctg cgctacggtg gcgtacttat cgtcactcag cgtgtgcttg atatacggag
    14401 cctcacgcaa ttgcttgagg tagtcgctca gacgcatccc ttctaccagt cgcagcggga
    14461 actgtgcttc tttaccgctt tccaacaatt tcagcatctc gcgcacggtc atctgcggtg
    14521 taaagcggta agtcccggct ttaaagtgag aaagatccgg ttcgatacgc agcagccatt
    14581 gaaaaacccg tgggcgattg atgatcttat cagcataaag ctgttcaccg agcgccagac
    14641 gtccggtccc tggcttcagg gtaaatatcg tctcttcttt gataagcaat ttgctgtcgg
    14701 caagatggcg aaccttccag acgcccacac cagcggcgat acccagtacc accagcaata
    14761 acaagattat caataacact tttttcatga ctaattcggg cgctcacaaa gtggggctaa
    14821 atattcatat aacgttgctg acgaaaagga gacatcgcca caggcacgta cgggcatcac
    14881 tggcattaac gcattacaaa taaccatctc atctgcctgc aacgcctctt ccagagaagc
    14941 ttgcacttcg acaagctgat aagaggattg tgccagcaaa cggatacaga attgtcgcat
    15001 aatgccgtta acacctgcct gatccagtcg cggcgtataa actacgttgc ccttccgcca
    15061 gaacaaatta gccgcacagc attccgtaac ccacccttcg ctgtcaagga ccagcgcctc
    15121 atcagcgttt gtctgctcaa gatgagagcg aatcaatact tgctcaagac gattgagatg
    15181 tttaatacct gcaagatgag gattgcgccc cagccgcacc gggcttagcg ccaacgtcat
    15241 cccctcgtta cgcaaacggt cgtaatgtgc aggataagcc gtaacggaga gaatccgcgt
    15301 tgccggtcca ctgttcaatg tgctgtaccc tcgcccgcca ctaccgcgac tgatcacgac
    15361 tttcagtaca ccattttgct gttctgctgc cagcgttttc atctcttgtt caagctgtgg
    15421 ccagaagtca caggaaatca tcaaccgctg acaagcatcc tgtagtcgct ggatatgcgc
    15481 cgataacaaa ctgactttac cgtcgataac tctggcggtg gtaaaacaac catcaccaaa
    15541 ctgcgttgcc cgatcgctta ctgccagcga ttcctgctta taaccgttaa ttaagaacat
    15601 accggctcct tatcagagaa ttgttagtgt ggcagcatgt tcactacgga acaagtcgga
    15661 ataaaagcta agaaaaaagg cccgcaagcg gaccttttat aagggtggaa aatgacaact
    15721 tagatctttt taaagatcaa agaaccatta gtgccaccga agccgaagga gttacacagc
    15781 gtgtattcca ttccgctgac ctgacgtgct tcatgcggta cgaaatccag atcgcaacct
    15841 tcatccgggt tatccaggtt gatggttggc ggaacagcct gatcgcgcag cgccaggatg
    15901 gagtagatag attctactgc acccgccgca cctaacagat gaccggtcat agatttcgtg
    15961 gagcttacca acacacggct tgcagcttca ccgaagatgg ttttcaccgc ctgcgcttca
    16021 gctttatcgc cagccggcgt agaagtaccg tgcgcgttaa cgtagccaat ctgactcgct
    16081 tcaatgcctg catcacgcag agcatttgcc attgccagag ctgcgcctgc gccattttct
    16141 ggcggtgacg tcatatgata agcatcgctg ctcataccaa agccgacgag ttcagcgtaa
    16201 attttcgcac cgcgtttttt cgcgtgttcg tactcttcaa gtaccagcat accggcacca
    16261 tcgcccagta cgaagccatc acgctcttta tcccacgggc ggctcgccgc ttgcgggtta
    16321 tcattgcggg tagataatgc acgtgccgcg ccaaaaccac caacacccag cggcgtactg
    16381 gctttctctg cgccacctgc aaccatcacg tcagcatcgc catacgcgat aatacgcgca
    16441 gcatgaccaa tgttgtgcac gccggaagta caggcggtcg cgatagagat gcttgggcca
    16501 cgcaggccat acatgatagt cagatgacct gccaccatgt tcacaatcgt tgacggaacg
    16561 aagaatgggc tgatcttacg tggaccaccg ttcatcagag atgtgtggtt ttcttcgatc
    16621 agtccgaggc cgccaatccc ggagccaatt gcggcaccaa tgcgggttgc gttctcttcc
    16681 gttatttcaa ggccagaatc ctgcatggcc tgaacgccag cgacaattcc atattgaatg
    16741 aaggcatcca tcttgcgctg ttctttgcgc gagataatgt cctcacagtt aaaatccttt
    16801 actaagccag caaatttcgt tgcataggcg ctagtatcga aatggtcgat taggctgatg
    16861 ccactctgac cggcaagcag agctttccag gtagactcta cggtattgcc gacaggagac
    16921 aacatgccca gtccggtcac aactacacga cgcttagaca cgtttgtcct ccagggaggg
    16981 aaaaaatgat tctagtggga caaaaagata aaactcaggc ggtcgaacga ccgcctggag
    17041 atgttcactt acgcctggtg gccgttgatg taatcaatgg cagcctgaac ggtggtgatt
    17101 ttctcagctt cttcgtccgg aatctcagta tcaaactctt cttccagagc cattaccagc
    17161 tcaacggtgt caagagaatc cgcgcccagg tcttcaacga aagaagcatt gttggtaact
    17221 tcttcctgct taacgcccag ctgttcgccg ataattttct taacgcgttc ttcgatagtg
    17281 ctcatactct taaatttcct atcaaaactc gctttcgcga tggttttcgt agtgtataaa
    17341 atgttgaaaa atttgcaact aaatcccggc aggtcttacc acgattttac gttattttga
    17401 ggccttaccc cccaataacg caaatcattt tgcactaatt attactcata accacgcaga
    17461 ctgcgcgcaa catgagcatt ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc
    17521 acatgcaaag tttcacccgt gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg
    17581 ttggcgattt cctgtgcgcc gccgaggcga cccgcaggaa cctgcgccag gatacccgca
    17641 cgctggtcat cgctcagcgc acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg
    17701 tttacagtaa taccgcgtga cgcaacttcg cgcgccagtg atttactgaa gccgatcaag
    17761 cccgctttcg ccgcagcgta gttggcctga ccgccatttc ccatggtacc aaccacagaa
    17821 ccgatagtga taatacgacc atgacgcttt ttcatcatag cgcgcattac cgcttttgac
    17881 agacggaaaa cagatgaaag gttggtttcg ataatatcgt tccactcttc atctttcatt
    17941 cgcattaaca ggttatcacg agtgataccg gcattattga ccaggatatc cacttcacca
    18001 aattctgcgc gaattttttc cagaacagat tcgatagatg ccgggtcggt cacattcaac
    18061 atcagacctt tgccgttagc acctaaataa tcactgatcg cctgagcgcc attttcactg
    18121 gtcgcagtgc caataacttt cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca
    18181 attccgcggc ttgcaccggt taccagtgcg atttttcctt caaaattcat gattttcctc
    18241 ttttaaagct cgagcgccgc tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg
    18301 gtgtcgacaa tgcgtttcgt caggccagtt agcactttgc ccgggccgac ttcatagaga
    18361 tgttctacgc cttgcgctgc catgtactca acagacttcg tccactgaac cgggttatac
    18421 aactgacgta ccagtgcgtc acgaatggca tcaccattgg tttcgcattt cacatcaacg
    18481 ttattcacaa caggaactgt tggtgcgtta aaggtgattt tcgctaattc tactgccagt
    18541 ttgtcggctg ctggtttcat cagcgcacag tgagacggta cgctcactgg taacggcagc
    18601 gcgcgttttg cgcccgcagc tttacaggca gcgccagcac gctcaaccgc ttctttatga
    18661 ccggcaataa ccacctgtcc cggagagtta aagtttaccg gagaaacgac ctgaccttct
    18721 gcagcttctt cacacgcttt cgcaatagac gcatcatcca gaccgatgat tgccgccata
    18781 gcgccagtgc cttccggtac ggcttcttgc atgaacttac cgcgcatctc aaccagacgc
    18841 accgcatcag cgaaatcaat cacgccagcg caaaccagcg cggagtattc ccccaggctg
    18901 tgaccggcca tcattgccgg tgctttaccg ccctgctgct gccatacgcg atacagcgca
    18961 acagatgcag tcaacagcgc tggctgggtt tgccaggttt tattcagttc ttcagctggc
    19021 ccctgctggg tcagcgccca caggtcgtag cccagcgccg cagaagcttc agcaaacgtt
    19081 tcttcgacaa ttggatagct cgccgccata tcagccagca ttccaacggt ttgagaaccc
    19141 tgtccaggga acacaaatgc aaattgcgtc atgttttaat ccttatccta gaaacgaacc
    19201 agcgcggagc cccaggtgaa tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc
    19261 ttaatgcgcc cgtcgcgtac agcttcatcc agcgcgcacg ggacagaggc cgcagaggta
    19321 ttaccgtggc gatccagcgt caccacgaca ttgtccatag acataccgag ttttttcgcc
    19381 gttgcactga taatacgcag gttagcctga tgcggaacca gccagtccag ttgagaacgg
    19441 tcaagattat ttgccgccag cgtctcatca acgatgtgcg ccagttccgt taccgcaacc
    19501 ttgaagactt cgttgcccgc catcgtcaga tgaattgaat tctctggatt cacacggtca
    19561 gcattaggca gcgtcagcaa ctcaccatag ctaccgtcgg catgcagatg ggtggagatg
    19621 attcccggct cttcagaggc agccagcacc gcagcgcccg cgccatcgcc aaaaataata
    19681 atagtcccac gatcggttgg atcgcaggtg cgcgccagta catcggaacc gacgaccaga
    19741 gcatacttca ccgccccaga tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa
    19801 cctgcgcagg ctgctgcaac gtcaaatgcc gggcaacctt taatgcccag catgctttga
    19861 atctgacaag ctgcgctcgg gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg
    19921 ccaatctggt ctttctcaat gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc
    19981 atggttgaaa cggtttcgtt tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg
    20041 acaatccact cgtcagaggt gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt
    20101 tcgggcagat agctgccagt accaataatc ttcgtataca tgtacgctca gtcacttttc
    20161 ggttatatac cgtcacttgc aaactgcgag ttcgctggca gcgtcctgct accgcagagt
    20221 tccgcttttg ccaccgtcca gcagctcaaa accagctggg tatacagatt ccaggcgagc
    20281 ggcaattcgc tgaggaactt gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc
    20341 aaaagctcgc tgattggctg caccatgact ttttatcacc gtgccgcgca atcctaacag
    20401 acaggcgccg ttatactggt cggggttgag gtgactgaat cgcctcgtca ggctcttttg
    20461 tagccaacgc tttaataaca gtagccacca cgaccgtttt ttcccttcac cctgagattt
    20521 cagcagagaa aggaacatcc tgacaacacc ttccatcgtc tttaacgtga catttcctgt
    20581 aaagccgtca caaaccagca catctgtctt gccagttaac aactcattgg cttcaagata
    20641 gccgatataa ttgatagaag ggattgtttt aagcaccgct gaggcatccc gaatactgtc
    20701 gagacccttt acttcttctt caccaatatt gagcaacgcc acgcgaggat tgggaatttc
    20761 caccacctct tcagccagga ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc
    20821 acaatcgacg ttggccccta aatcgagaac caccgttttg cccttttgct gatgcggcaa
    20881 taccgtcacc agcgccggac gctcaatccc ctccaggggc ttgagtaata attttgccag
    20941 ccccatcagt gccccggtat taccggcact gacacaggct tgcgctcgac cttctttcac
    21001 caactccagg gccacgcgca ttgaactccc acgactggcg cggatagctt gcgaaggccg
    21061 gacatcactg gcgataactg actgcgcagg aataatctgc agacgcgaac gttgttcaaa
    21121 gtcagcttta gcaagtaatg gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag
    21181 ttgcgaatta gaattcagtg cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc
    21241 ccctcccatg acatctaacg ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt
    21301 ggtctttccc cagttgcctg gggaaaatcc tcactaagct tcatcacgca gacgcgtgat
    21361 tacttagcga tgaccttgcg gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg
    21421 tgtttttcac cagaagtttt gtctacagac aggctggtga ctgcggtcag cgcgtcatgg
    21481 gaacgacgca tgccacgttt ggaacgggtt ggtttattct gttgtacggc catggacctt
    21541 actcctcaat tacggtttat ccccgatcca acggggagca ccaattactt acgctttaag
    21601 ctggctaata cggcaaatgg gtttggcttt tgcgcttctt caggcagttc accaaagacc
    21661 atgtccgctt cggacacttc acagtgttca gaatcatgca ccggaactac cggcaaggcg
    21721 aggatgattt catcttcaac cattgcaagc agatcgattt caccgaattc gttaacctca
    21781 atcggttcat acgcttccgg cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa
    21841 caatacgttg tgtagacctg atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt
    21901 accgtcacct tcgcatcgcc gtttaacact gcgagacgtt ggttatcgat agcgaacgac
    21961 atggagcatt ccacatcact gtccacactg actacggatt cggcgacgcg ctcaacctga
    22021 tcaggggtat agataccctg gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga
    22081 gtcaggggta attttacctt ttgcataggg cgcgcatatt aactttgtaa cgtcatagag
    22141 tcaaagaaaa aggcagcctg tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta
    22201 ctgctttatt ttcgttcagt gctgataacg aacatcaacc tgcaaaacga agcgcatagt
    22261 ttaaaatggc tatcatcaat acgctatatc tgatggaaaa aaatatgcct aaacttattt
    22321 tagcctccac ctcgccatgg cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat
    22381 gtgcagcacc agaggtcgac gaaaccccac gcagcgacga atcaccgcga cagttggtgc
    22441 ttcgactggc acaagaaaaa gcgcaatctc tggcgtcacg ttatccggat catttaatta
    22501 ttggttcaga ccaggtatgt gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag
    22561 aaaatgcccg tctgcaatta cgcaaagcca gcggcaatat cgtcaccttc tatactggac
    22621 tggcgttgtt taattcggcg aatgggcatc tacaaacaga agtggagcct tttgacgtcc
    22681 atttccgtca cctgagcgag gcggagattg ataattacgt gcgtaaggag catcccctgc
    22741 actgcgcggg tagctttaag agtgaaggat ttggcattac gctgtttgag cgtttagagg
    22801 gacgtgaccc taacacgctg gttggtttac cgctaatcgc gctgtgtcag atgttacgtc
    22861 gggaagggaa aaacccgctg atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc
    22921 cggcaatcgg tgcaggcggc ctgatgcggc gtaaacgcct tatcaggcca accgcccatt
    22981 atcaggctta tatgcttgta ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa
    23041 ccttcatcca tcggcgcttc gatacgcatc acctcaccgg tccccggatg ggtaaacttc
    23101 aacgctgcgg cgtgcaggaa cagacgattt aatcccgtgc ctgcttcagt gagctgtctg
    23161 tcaaattcac ggtcaccgta gcgatcgtca aaggcaatcg gatgacccgc atactgtgta
    23221 tgcacacgga tctgatgagt acgtccggtt accgggctac aacgcaccag ggtggcaaat
    23281 gcatagcgtt cttccacttt aaagcgtgtt tccgacggtt tgccttcctg actgacacgc
    23341 acgatacgtt cgccgctttg cagaatattt ttcaataacg gcgcttgaac gctcttcaca
    23401 tgcgactgcc actgaccgcg caccagcgcc aggtaatctt tttgcattcc tttttcacgt
    23461 aattgctcat gcagagaacg caacgccgag cgttttttcg ctaccagcaa cacacctgaa
    23521 gtgtcccggt cgagacgatg aaccagttca aggaaccgcg cttccgggcg caacgcccgc
    23581 aaaccttcga taacgccgaa gcttaaacca ctgccgccat gtaccgccgt accggaaggt
    23641 ttattcagca ccaggatatg atcatcttca tataagatga cgtccgccag cgccgccacc
    23701 ttttgcagat gtggcgaaac cgcctcttct tcccgctcag caacgcgaac cggtgggata
    23761 cgcacctcat cacccgcttc gagtttatat tcaggcttaa tacgtttttt gttcacccgc
    23821 acttcacctt tacgcaaaat acggtaaatc atactttttg gtacgccttt caattgggta
    23881 cgcaaaaagt tatcgatacg ttgccccgct tcgtcagcgg tgatagcaac aatttttacg
    23941 gatggagtct ctgttttcat ggtgggcgat tctaattagc caacaggatt cgcgccactc
    24001 atttttctat gcttatattt agttttacac cttattactt cactacgtga tcactttatt
    24061 gatggttatt aaaccaatca ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa
    24121 atggtaaaag tcatcttgct ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg
    24181 tccatccttg ttaaaacaag aaattttacg gaataaccca ttttgcccga ccgatcatcc
    24241 acgcagcaat ggcgtaagac gtattgatct ttcaggcagt tagcgggctg cgggttgcag
    24301 tccttaccgg tagatggaaa tatttctgga gagtaatacc cagtctgttt ctttgataat
    24361 tgcgctgttt ttccgcatga aaaacgggca accgacactc tgcgcctctt tgagctgacg
    24421 ataaccgtga ggttggcgac gcgactagac acgaggccat cggttcacac ccggaaaggc
    24481 gttactttgc ccgcagctta gtcgtcaatg taagaataat gagtaagtta cgatgaaaag
    24541 aatgttaatc aacgcaactc agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg
    24601 tctgtatgac ctggatatcg aaagtccagg gcacgagcag aaaaaggcaa acatctacaa
    24661 aggtaaaatc acccgcattg aaccgagtct ggaagctgct tttgttgatt acggcgctga
    24721 acgtcacggt ttcctcccac taaaagaaat tgcccgcgaa tatttccctg ctaactacag
    24781 tgctcatggt cgtcccaaca ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca
    24841 gatcgataaa gaagagcgcg gcaacaaagg cgcggcatta accaccttta tcagtctggc
    24901 gggtagctat ctggttctga tgccgaacaa cccgcgcgcg ggtggcattt ctcgccgtat
    24961 cgaaggcgac gaccgtaccg aattaaaaga agcactggcg agccttgaac tgccggaagg
    25021 catggggctt atcgtgcgca ccgctggcgt cggcaaatct gctgaggcgc tgcaatggga
    25081 tttaagcttc cgtctgaaac actgggaagc catcaaaaaa gccgctgaaa gccgcccggc
    25141 tccgttcctg attcatcagg agagcaacgt aatcgttcgc gcattccgcg attacttacg
    25201 tcaggacatc ggcgaaatcc ttatcgataa cccgaaagtg ctcgaactgg cacgtcagca
    25261 tatcgctgca ttaggtcgcc cggatttcag cagcaaaatc aaactgtaca ccggcgagat
    25321 cccgctgttc agccactacc agatcgagtc gcagatcgaa tccgccttcc agcgtgaagt
    25381 ccgtctgccg tccggtggtt ccattgttat cgacagcacc gaagcgttaa ccgccatcga
    25441 catcaactcc gcacgcgcga cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa
    25501 cctcgaagct gccgatgaga ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat
    25561 tgttatcgac ttcatcgaca tgacgcctgt acgccaccag cgtgcggtag aaaaccgtct
    25621 gcgtgaagcg gtgcgtcagg accgtgcgcg tattcaaatc agccatattt ctcgctttgg
    25681 cctgctggaa atgtcccgtc agcgcctgag cccatcactg ggtgaatcca gtcatcacgt
    25741 ttgtccgcgt tgttctggta ctggcaccgt gcgtgacaac gaatcgctgt cgctctctat
    25801 tctgcgtctg atcgaagaag aagcgctgaa agagaacacc caggaagttc acgccattgt
    25861 tcctgtgcca atcgcttctt acctgctgaa tgaaaaacgt tctgcggtaa atgccattga
    25921 aactcgtcag gacggtgtgc gctgtgtaat tgtgccaaac gatcagatgg aaaccccgca
    25981 ctaccacgtg ctgcgcgtgc gtaaagggga agaaacccca accttaagct acatgctgcc
    26041 gaagctgcat gaagaagcga tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg
    26101 tccggaacaa cctgcgctgg caacctttgc catgccggat gtgccgccag cgccaacgcc
    26161 agctgaacct gccgcgcctg ttgtagcccc agcaccgaaa gctgcaccgg caacaccagc
    26221 aactcctgca caacctgggc tgttgagccg cttcttcggc gcactgaaag cgctgttcag
    26281 cggtagtgaa gaaaccaaac cgaccgagca accagcaccg aaagcagaag cgaaaccgga
    26341 acgtcaacag gatcgtcgca agcctcgtca gaacaaccgc cgtgaccgta atgagcgccg
    26401 cgacacccgt agtgaacgta ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg
    26461 tcgccaggca cagcagcaga ctgccgagac gcgtgagagc cgtcagcaag ctgaggtaac
    26521 ggaaaaagca cgtaccaccg acgagcagca agcgccgcgt cgtgaacgta gccgccgccg
    26581 taacgatgat aaacgtcagg cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc
    26641 tgttcaggaa accgaacagg aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg
    26701 tcagctcaat cagaaagtgc gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc
    26761 ggtggttgaa gaaactgccg ctgccgaacc aattgttcag gaagctccag ctccacgcac
    26821 agaactggtg aaagtcccgc tgccagtcgt agcgcaaact gcaccagaac agcaagaaga
    26881 gaacaatgct gataaccgtg acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg
    26941 tcacctgcgc gtaagtggtc agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca
    27001 gtcgccaatg ccgttgaccg tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg
    27061 gatccgctat ccaattgtac gtccgcaaga tgtacaggtt gaagagcagc gcgaacagga
    27121 agaagtacat gtgcagccga tggtgactga ggtccctgtc gccgccgcta tcgaaccggt
    27181 tgttagcgcg ccagttgttg aagaagtggc cggtgtcgta gaagcccccg ttcaggttgc
    27241 cgaaccgcaa ccggaagtgg ttgaaacgac gcatcctgaa gtgatcgctg ccgcggtaac
    27301 tgaacagccg caggtgatta ccgagtctga tgttgtcgta gcccaggaag ttgcagaaca
    27361 agcagaaccg gtggttgaac cgcaggaaga gacggcagat attgaagaag ttgtcgaaac
    27421 tgctgaggtt gtagttgctg aacctgaagt tgttgctcaa cctgccgcgc cagtagtcgc
    27481 tgaagtcgca gcagaagttg aaacggtagc tgcggtcgaa cctgaggtca ccgttgagca
    27541 taaccacgct accgcgccaa tgacgcgcgc tccagcaccg gaatatgttc cggaggcacc
    27601 gcgtcacagt gactggcagc gccctacttt tgccttcgaa ggtaaaggtg ccgcaggtgg
    27661 tcatacggca acacatcatg cctctgccgc tcctgcgcgt ccgcaacctg ttgagtaata
    27721 attagctcaa agtaatcaag ccctggtaac tgccagggct tttttatttc atctttgaat
    27781 caacatatta aaattcatcg aatggcatcc ttgctaacca acaatgcaaa ataggcaaaa
    27841 aaatgcccgc cccagcgatc ccagagcagg cagacaaaac atacccagtt tcatgatatg
    27901 ttttaaagcg aaattatttg ttgagctgga agagcgacaa tccctgcata tcggtaaatg
    27961 ctttatacga tgcctgcaat gccgtttgct gcatgatgta agatgaaata gttgcattcc
    28021 agtccacatc aaccagatcg ctcatctgct gcgtttgccc taaagcgcga tcgctaccta
    28081 atgaatccag cgactccagt tcgttcagct gcgtgcctaa ttccgcgcga acagtcagca
    28141 cattgttcag cgagtttttc agtccgcggt tggttttatc taacgccgca gcggcggttt
    28201 ctttatccgc ttcgctatcc gcgaccggcg ttttcagggc tgcgatggca ctatccagca
    28261 tggcaaaaag attggtttca gaagcgctac cgtctggttc cgctaccgcg ttgctggtaa
    28321 tactgtcgaa aattttgtca cccgtgtgcc ctatcaccat cgaacgcgaa gcatcgacct
    28381 gttgtttaat actttctgct ccaccgacgt atttcccttt ctcttcgcta aacggcgcag
    28441 tctctgtttt ataaccggca aaaatgtagc gcccgttacc gtcagtggtg ttcgccagat
    28501 tcagcaactg gtcacgaagc ccctgaatat ccgtagccag cgaggcccgg tcatcgtcac
    28561 tcaaggtgcc attgctggcg tagacaattt tttcctgagc attctggata gcagtggtga
    28621 cctggctaag tacactctct tccagtgaca ctttttgagt ggcgaaagta cgtgccagcg
    28681 tgtactggct gttttgcgcc tgtgcctggg agagaacaac agcttgtgat gcagcaatgg
    28741 gatcgtctga agggttaacg actcgcttac ccgtcgacat ctgctcgccg tacttcatcc
    28801 attctgcctg agaattggtg atgccgcgca tgttttgctg gtacatcatc tgggtactga
    28861 aacgcatttt atctcccctt agcgaatgtt aatcagcgca tcaaaaatcg cgtttgccgt
    28921 ctgcagaacc tgcgcattcg ccaggtaata ctgctgaaaa cgttgcagat ttccgtactc
    28981 ctcatcgaga ttgacaccgg aaatcgactg ctgctgattg gaaagctgcg tcaccacatt
    29041 accttgcgtg gtgctactgg ttttcaacgt cgcggtttta ttaccgatat cactcactaa
    29101 cgaggcataa gcgtcgttaa aggatttcgc accgcccacc gttttactgt tgctttgcag
    29161 atccagcagg gcctgaccgt tgcggttgtc gctatcaccc gcatcttctt cgctcgccat
    29221 cgcgattttc gcttcgtcgg tgattaatac atccatgttg acgatggcgt cacttactgg
    29281 tttcagcgtg aagctgtcgt taacggcagg cgttcctgta aacgtcaact ccagaccatc
    29341 aaatgccact ttaccgttgg catctggtgt cgccgtaaaa gtggtattgc tggcaaggcg
    29401 ggtgacctgc cactgattat tatcgaacga gattttgtaa tctgtcgcca gtaccacgga
    29461 ggcatcagtt accgtggcac cgatcgcaac gtcacctttg tttttcgtgt tttgcagaac
    29521 cgcgggctta ccgatagtaa agaaatcttc accagcatcg ccgttagcat caaatccggc
    29581 tttgtgttgg ctgttgaaag cctcggcaaa tgccagcgcc agttgtccaa gcgtattacg
    29641 cgtctggtcc agatcctgag aacggaatgt cagaatgccg cccagcgacc cggtattcag
    29701 taatttctcc gggatctcaa tattgcctgc cgtcccatca acgtaagcga cagtcgtacg
    29761 agaagggtcg gcgctggaag gaactgccgc cagttgccgc gccgtacttc cctgaaccag
    29821 tgagtaacca ttggccatcg tgatgttata agtaccgcca tcctgaacgc tgacttctac
    29881 gccaacaatc tggtttaatt cgctcaccag ttgatcgcgt tgatccagca gattgttagg
    29941 tgacgcccct gcccccacgc ctgtcaggcg cgagatttga tcgttcaggc tggcaatttg
    30001 tttagcgtaa ttgttgatct gatcaacgct ggcacctatc gcgatattga cctgtttgtc
    30061 ctggtcgcgc agatattgat cggtggtttt aaactgattc accaaccctt ctgatttccc
    30121 aatcagcgcc tggcgcgctg ccgggtcttc cgcgttactc accagcgttt gcaggctggt
    30181 gaagaaatcc tgcatctgtg ttgccagcga agaggtactg gtggagagca tattgtcgat
    30241 tttcgacatc tgctcatagc gggcagtcag gccgctactt tgcgtctgcg cagcacgtaa
    30301 ctggttggta ataaacgcat catactcacg ctgcacacca gaaacgtaga cgccattgcc
    30361 aacccagccg ccagcgccca acgtgctatt ggcctgcgcc ataatagtgg tttggcgggt
    30421 atatccggca acgttatagc tggagatatt attacttgcc gtatttaacg ccgcctgggc
    30481 cgcgttcagt ccgctcatgg cgttattaat caagctggac atggaggttc cttataagcc
    30541 ttcaattaca gagtattatc ggcagcgacc cgccggactt gagttattca gaacagatta
    30601 tcaatgttca tactgtaggt tttgctcacc ttgtcgctta tcgatttcat ctgctgaatc
    30661 atgttggtga gtttgcgggc atagtgagga tcggtggcat agcccgcgtc ctgtagggcc
    30721 tgcgccccct gttccgcact cgcggcggtc gtcacggcgg cgtagcgcgg gttacgcgtt
    30781 aacagcccaa cgtaatccga caaggcttcc agatacgagc tgtagacgcg aaacttcgct
    30841 tttactttct tcgcttcgcc gttttcatat tcagtcgtgg tgatttcagt aactggccct
    30901 ttccagttgc cagaggcttt gacaccaaac aggttatagc tcggctcgcc gttttcgcgg
    30961 cggatttgcc gttgccccca accagattcc agtgccgcct gagcgaggat caaatgatgt
    31021 ggcacaccgc tttgctggct tgccagttgg gcgggcagcg agagttgcgc gaggaatgct
    31081 ttactgtcac ccggcagcga atcatcgtag ttacgtggca cggccttttg caccagctgc
    31141 gaaagcgcct gattttgata acgcaccaca gtttcgagcg ggaatttcat cggtgctgct
    31201 ggcgtggact cctctggcaa tggttgttct ggcgtcatct gtttaaccat catctctgca
    31261 agccccagac ctttgcccgc cgtcatctgt tgggcaattt gctggtcata catactggta
    31321 tacagacgag tgtgctcgct gctgaacagg ccatcttttg gtaaagcgtc gcgcatgctt
    31381 ttcaacatca tctgcacgaa catcccttcc acctgacggg ccaccggacg gatatttgcc
    31441 gccggatctt cgcccacttt cgcctttagt tcgttgagtg attgcgcatc ccaggccgca
    31501 cttgccagta gtttgctgtc gctgatcatc agatgatttc cagttttgcc cgcagacatc
    31561 ccgcactttg cattgattgc aaaatagaca tcagatccat cggcgtagcg cccagcgcat
    31621 tgagcgcgcg caccacgtta ttgaggctgg cgctggaacg tacgctttgc agcgaaccgc
    31681 cgctctggcg taaatcgatc tgcgtttgtg gagtaaccac agtctgtcca ccaccaaacg
    31741 gtgtatctgg ctggctgaca ttggcctgac gattgactgt tactgagaga tttccctgcg
    31801 ctaccgcgca gctgtcgagg gtcacttcgc gattcatcac caccgaaccg gtgcgcgagt
    31861 taatcactac tttagcgtcc tgcggggtga cattaacctg catattctgg atatctgcaa
    31921 ggaaacggac ctgggaactg ttgccacttg gtacgcgcac ctgaatagtc cgcgcatcta
    31981 acgcggtggc gctgccatat ccacgcacgc ggttgatggt gtcagcgatt tgctgcgcca
    32041 tgctgaaatc ttcgtcgtta agttgcaaat taagggtatt cccgacgcca aactggctgg
    32101 gcaattcacg ttcaataacc gcaccattgg tgatccgtcc accgttcagt tggttcacct
    32161 gaacactgct accgccagcg gaggctcctg cgccgccaac cagaatattg ccctgcgcca
    32221 gcgcatacac ctgactgtca acgcccttaa gcggcgtcat caacaacgta cctccacgca
    32281 agcttttggc atttcccatg gaagaaacca ccacatcgat ggtttgcccc tgacgtccaa
    32341 acggaggaag tgacgctgtc accattaccg cagcgacgtt ttttagctgc atattggtgc
    32401 ccgtcggaac ggtaattccc agctgtgaga gcatgttatt aagcgtttgt gtggtaaacg
    32461 gcgtctgggt tgtctggtca ccggtgccat ccagccccac caccagacca tagccaatca
    32521 gtgagttttg ccttaccccc tgaacactgg tgagatcgcg aatacgctca gcctgagccg
    32581 ccgtcgtgac cagtagaaga attaatgcag agagaaattt aatcaccaca gcctcactta
    32641 cattggcgac aggttaagga agaaacgctg caaccagccc atattttgcg cttcgttaat
    32701 gtagccattg cctacgtatt caatgcgcgc atccgccacc tgagtagacg gtacggtatt
    32761 gctgccgctg atagtgcgtg gattaaccac gccagagaag cgaataaatt cggtaccctg
    32821 attaatggca atctgttttt cacccaccac atgcaggttg ccgttgacca gtacctggtc
    32881 aaccgtcacc gtcaacgtgc cgctaaaggt attgctggca ttggccccgc cctttccgtt
    32941 gaacgtgtta ccaccggagg cttcgacatc ggcacgagcg ttaccaaaca gcccctgcaa
    33001 atagcgcggc acagtatcaa agccaaaatt agttttaccg tcacggctgg cattcgcaga
    33061 ggagcttttg ctggcgctga cgttctcctg caacacgatg gtcagcgtat cgccaatatt
    33121 gcgtggtcga cgatcttcaa acagcggttg atagccatag ttaatcggct gagcagactg
    33181 gaaaatagaa ccgttggcga cgggcgtcgg accgggaacc ggttgtgcac tggtcgcccc
    33241 ctgcaccagc ggcgtggagg gtatccaggc gcagccggtt agtgaaagca ccaacaagct
    33301 ggaaatggca taagtatgcg cagcgttttt ttgcatggct atcatcttca aaaatcagta
    33361 aaccggtgaa cctgccaccg gttaagcctt agagttgcgt cagtttttgc agcatctgat
    33421 cggtggtgga caccgcttta ctgttgattt cgtaagcgcg ttgcacctga atcatattga
    33481 ccagttcttc cgccacgttg acgttagacg tttcaacata cccttgatac agcagtcccg
    33541 cgccgttcag gcccggcgtg ctttcgttcg gtgcaccaga ggattgcgtt tcggtgtaga
    33601 ggttttcgcc aatgctctcc agcccggtgt cattcataaa ggtggtgaga ttgagctgcc
    33661 caacctgaac cggagctgcc tggccttgtt gggttacgct gaccacgcca tcacgaccga
    33721 tggtgatact taacgcattc gccggaatgg tgatcgctgg ctgcacctga aaaccaccag
    33781 ccgtcaccag ctgcccgttc tgatccacct ggaaagagcc gtcacgggta taggctgatg
    33841 aaccatctgg caacatcacc tggaaaaagc cctgcccttt aatcgcgaca tctttgctgt
    33901 tgttggtctg cgacaggttt ccctggctgt gtaagcgttc agtggcgacc gggcgtaccc
    33961 ccgtgccgat ttgtaatccg gagggtaagg tggtttgttc ggaagactgt gcccccggct
    34021 ggcgaatggt ttgataaagc agatcttcaa acaccgcgcg ctgacgctta aaaccgttag
    34081 tactgacgtt tgccaggttg ttggcaatga cgtccatatt ggtttgctgg gcgtcaaggc
    34141 ccgttttggc gatccataat gaactgatca taaaatgtcc tgtagtggat tagtgacaag
    34201 cagagcagaa gcacgcgaca gtgtgaattt cccctcaccc taaccctctc cccaaagggg
    34261 cgagggaata gtccgcgcgc ttttattgca atgtcatcaa tcgttcaatt aactcatcga
    34321 cagcagttgg ttggcacggc ctgcgttatc atcgacgctg ctgatcacct tcatctgcat
    34381 ttcaaaacgc cgcgcgctgg caatcatgtc gctcattgcc gcaacggcat tgacgttact
    34441 gccttccaga acccccgaca tcacacgcaa ggttggatct gcctgcagta tcggcccacg
    34501 cgtggcctgg ctttctgcgc ttaaacgaaa aatgccgtcg tcaccgcgtt gcacttcgct
    34561 gcccgtggct ttcaccagtt tcagacgccc cactggcgca accgtatttg ccggatcgcc
    34621 cggattgagc gccgagattg tgccatcggc agcaatagtg atttccgccc cttccggcac
    34681 agcaattggc ccagcctcgc ctatcaccgg atgcccctga attgtcagtt gcccggtggg
    34741 atcaacctga atgctgccat tacgcgtata cccttcgctg ccgtcagcgg actgcacggc
    34801 cagccagcca tcctgctgca atgcaacgtc cagcgggcgc gaggtgtaat ccatcttgcc
    34861 gggcgtcatg tctgcacccg gtgttgacgc cgttaccagc gtgcgcgtgg gcagtgaaag
    34921 cccttccacc ggcactgcac gcaaggcgtt taactgcgcg cgaaaaccgg gcgtagaggc
    34981 attggccaga ttactggcgg ttaccgcctg ttgattcagc gtctggctgg ctgctcccat
    35041 cgcggtataa attgcgtgat ccattgagct atcccgtcag cgattagcgt aagttaacca
    35101 gcgtgttgag gatctggtcc tgggttttga tggtctgggc gttagactga tagttacgct
    35161 gggcaacgat catattgacc agttctttac tgagatcgac gttggacgct tccaacgcac
    35221 cgttggtcag ggtgccaaag ttgcccgtcc cggctgtccc caacagcgcc acgccagaag
    35281 attgcgtcgc agaccagacg ttgtcgcctt cggatgccag accttcgttg ttggcaaagt
    35341 tcgccagtac aatctgcccc agcagttgtg tttgttcgtt ggaatagttg ccgacaaccg
    35401 taccgtcatc attgatttga taactcacca gatcgcccgg tttgtagcca ttctgggtgg
    35461 ttgccacaat attgttagcg ccggtatttt gctgcatgga gttgaggaag ctcaggctaa
    35521 acgtggcggg atctgcgccg ttaattgcgc cagtggtaat attctctgtt ggatttgagg
    35581 tcagaacgcc attggcatta aacaccagct tcattgcagg ctcggctgta cctgttggat
    35641 cactgctatc ctgggtgtag acctgccagt tattatcccc ggtcttcaca aagtagacgc
    35701 tcatgtcatg agcattaccc tgactgtcga aaacagtcac cgaacctttt ttgttatagc
    35761 tatccgcatt gctggcgctg aatggcgtaa cagtaggaag cggatcactg gaattcaggt
    35821 tgatctgcat cgatgccgtg gtggtagttt tcgctgccat cagggtattc gggatcgaaa
    35881 tattggtcgg attcgcccct tgctgaatag tcggcggcgt accggttgcc gggtaacccg
    35941 tcagctgtaa accttgcata ttcaccaggt tacggttttc atccagctta aattgtccgt
    36001 tacggctgta gaacaccgaa ccgttgctgt ctaccagacg gaaaaaaccg ttctggctga
    36061 tagcaacgtc cagacctcgc ccggtgttgg tggtcgtgcc atcggtaaag tcctgagtga
    36121 taccggcaac ttttaccccc agtcccactt tcgaaccggc aaacatatcg gcaaaagagg
    36181 ccgtgcctga tttaaagccg taggtggcgg agttggcgat attgttgcca ataacatcga
    36241 ggttggtggc ggcagcgttt aatccgctaa ccgcttgaga aaaggccatg actgactcct
    36301 gaaatgtgaa ggcttaaatt atctgccgta cttcgtcgag ggtggtggtg ccgtaagtgc
    36361 cgagatccag cgtattaccg ctgttgccgc ggatcacacc ctgcaccaga gcaaactgca
    36421 gcggctgggc aaccagttgt gtaccaccgt tactggcgct aatcgctaca ttgtaagaac
    36481 cgttcggcgc agtgctgccg tcagtcaacg taccgtccca ggtgaaactg tgaactccgg
    36541 cggtcagttc accaatatca atggtgcgca caaccgcacc atttttatcg gtgatggtgg
    36601 ccgtaacttt gtctgccgcc tgttgcagct caacaccaaa cggcgtggtc gtggtcacag
    36661 ctccttcttc actgccggtt cccgcaagaa cagtggtgcc gtggatcatc acgccgtgac
    36721 cgatcaggtt actggcctgt aacgactggc tgttatcaat ctgtccggaa atagatccga
    36781 gtgtggtatt gagtttttca atcccgctga ccgtgctgat ttgtgccaat tgcgacgtca
    36841 gctcgttgtt ttccattgga ttggtcgggt cctggttttt cagctgcgcc accagcaaag
    36901 tcagaaaact gctttgtaaa tctgcggcgt tgctgcccgt gagcgaacta ctgctggtgg
    36961 tactgacgcc ggtatttgtc ggatcggtgg tggttaccgc aatggacata gctttctcct
    37021 ttattgaccg agcgtaaggg ttttcagcat catgcttttc accgtgttga gcacttcaac
    37081 gttggcctga tagctgcgtg acgccgacat ggtgttaacc atctccccga cgacatcaac
    37141 gttcggcatt tttacgtagc cctttgcatc tgccagcgga ttacccggtt cataaaccag
    37201 tttgtccggg gcctgacttt ctataacatc gccaaccttt acgccgcctg tcgcagcacc
    37261 tggtgcagcg ttaacctgga ataccacctg ttttgcccga tatggctgtc catcaggacc
    37321 ggtcacgcta tcagcatttg ccagattact ggccgccacg ttcaggcgct gggactgggc
    37381 agttaacgcc gacccggcga tatcaaaaat attcagcagt gccatccgtt aatttccgct
    37441 ctgtaaaacg ttcatcatgc ctttgatttg cccgctcaac gcgctaaggc tcatctggta
    37501 ttgcaggctg ttatcggcaa actgggtgcg ttcgcgatcc atatcgacgg tattaccgtc
    37561 aagcgaaggc tggtccggaa tacggtattg cagttctgcg gtaggaggcg tcagcgcctg
    37621 cgccgggatg tgttgcgttg aggtcatcgt cagtgcaacc acactggttg catcccgtcc
    37681 acgttgcatg acttttttaa gttcactggc aaaatcgata tcgcgcgcct gataaccagg
    37741 ggtatcggca ttggcgatgt ttgctgccag cacttcctga cgctgggcgc gcagattgag
    37801 cgcctcttgt tgaaaacgta aggcggcgtc gagcttatcg agcatatctc ctccgcaggt
    37861 atcaaaattc tgccatcagc ttaaatgcct ttacgcgcgc gttatcggcg gaataaacgc
    37921 aaaatgggtc gctatttatg ccgttgatgg tcattgcgga caggtacaat tcacgttgta
    37981 gaaatggctg ggggcgaaaa tgctgacaat aaaacgtagc gtggcgatca tcgcgatact
    38041 gttcagtccg ttaagtacgg cgagcaatct cacatcgcaa ttgcacaact tttttagcgc
    38101 ccaactcgcg ggggtaagtg atgaggttcg tgtttctatt cgtacagcgc ccaatctact
    38161 accgccatgc gagcagccat tgctttcgat gagcaataat tcccgcctgt ggggcaatgt
    38221 gaatgtgttg gcacgctgcg gtaacgacaa acgatattta caggttaatg tgcaggccac
    38281 aggaaattat gtggttgccg cgatgcccat tgtgcgggga ggaaagctgg aagctggcaa
    38341 tgtcaaacta aaacgcggac ggctggatac cctgccaccg cgtacggtgc tggatatcaa
    38401 tcaacttgtt gatgccatta gcctgcgcga tctatcaccc gatcaaccta tccagttaac
    38461 ccagtttcgc caggcatggc gggtaaaagc gggacaacgc gtcaatgtga tcgccagcgg
    38521 tgatgggttt agcgccaacg cagaaggtca ggcgctgaac aatgcagccg tcgcacagaa
    38581 tgcgcgggtg cgcatggtat cgggacaggt agtcagcggc gttgttgatg cagatgggaa
    38641 tattcttata aacctgtaag ctgttaaaga ttacccgtcc cttgccgata aataagcaac
    38701 acatgataaa agcgccctca atgaggaata aaccatgagt attgatcgca cttcgcctct
    38761 gaagcctgta agcaccgttc aaccgcgcga aaccactgac gcgccggtaa cgaacagccg
    38821 ggcggcaaaa acaaccgcct ccaccagcac cagtgtgacg ttaagcgacg cgcaagcaaa
    38881 actgatgcaa cccggcagca gtgatatcaa tcttgaacgt gtcgaagcgt taaaactggc
    38941 gattcgtaac ggtgaactaa aaatggacac cggcaaaatt gccgatgcgc tgatcaacga
    39001 agcgcagcaa gacttgcaga gtaactgatc gtatgacacg tcttgcagag atcctcgacc
    39061 agatgtccgc tgtgcttaac gatctcaaaa cggtaatgga tcaagagcag caacatctct
    39121 ctatggggca gatcaacggc agccagttgc aatggattac agaacaaaaa agctcactgc
    39181 tggcgacgct ggattacctc gaacagttac gcaagaaaga acccaataca gcaaatagcg
    39241 ttgatattag tcaacgctgg caggaaatta ctgtgaaaac gcagcaacta cgccaaatga
    39301 atcaacataa cggctggtta ctggaaggac agattgagcg caatcaacag gcgctggaaa
    39361 tgttgaaacc gcatcaggaa ccgacgctat atggggcgaa cggtcagacc tcaacaatcc
    39421 atcgcggcgg taaaaagatt tcgatctgaa tattatctcc ggcctgcact gcaggccgga
    39481 atgcattgtt acaccgtccg gcgggcaaat tctttaactt tgaagcccag taccgccagt
    39541 gcagcgaagt acgcggcaat ccccgccagc acgaccgcca ttaaacgcag taagcgccag
    39601 ggcatggtac ccaatgacca ctccggcatg atatgtaaca tacctaaaag cacgccagac
    39661 atcaccagta ccgccaccac cagacgcaac agaaacgcca tccagccggg ttgcggggta
    39721 aagattttct gcttacgcaa ctgccagtaa agcagcgaag cattcagaca cgccgccaga
    39781 ccaatagaaa gtgacagccc ggcatgtttc aacggaccaa taaacgccag gttcatcaat
    39841 tgcgttaaaa tcagcgtaac gatggcaatt ttcactggcg ttttaatgtc ctggcgggaa
    39901 taaaagccag gagccaaaac tttcactaca atcaggccaa tcaaacccac cgagtaggca
    39961 attaacgccc gctgggtcat cagcgcatca aaggcggtaa atttaccgta ctggaacagc
    40021 gaaacggtca acggacctga aagaatgccc aacgcaaccg cactcggcag cgccaacagg
    40081 aaacaaagac gcagccccca gtccatcaaa cggttgtatt catcatgatt gccactggca
    40141 aaactcttcg acagcgacgg cagcaaaatg gtgccaagcg ccacgcccag cacaccagac
    40201 ggaaactcca ttaagcggtc ggcgtaatac atccaagaca ccgaaccgga agcaagaaac
    40261 gaggcaaaaa tggtgttgat gattaaggag atctggctca cagagacgcc aaggatcgcc
    40321 ggtcccatct gtttcaccac gcgcattgct ccggcatcgt ggaagttaat gcgcggcagg
    40381 accagcatgc cgatcttctt caggtgcggt agctgataca ccagctgcag gatgccgccg
    40441 accgtaacag cccacgccag cgccagcacc ggtgggttaa agtacggtgc ggcaaacagc
    40501 gcgaaaccaa tcatgctgat gttaagcagt gctggagcaa acgccggaat cgagaagcgg
    40561 ttccacgtat tcagaatcgc tcccaccagc gacgccaggg agatcagcaa gatataggga
    40621 aaggtaatct ttagtagctg gctggtcagg gcaaatttgt cggcagtgtc agcgaagcct
    40681 ggcgcggtca ccatgatcac ccacggtgcg gcgagcatgc cagcgaccgt cacaaccgcc
    40741 agcgcgagtg tcagcaggcc agaaacataa gagacaaaga cccgcgtggc gtcttcaccc
    40801 tgcttacttt tatattccgc cagaatcggt acaaatgcct gggaaaatgc cccttcggca
    40861 aagatacggc gtaacaagtt aggaagttta aaagcgacga aaaaggcgtc ggttgccatc
    40921 cctgcgccaa agattctggc gacaattgcg tctcgtgcga agccaagcac acgcgaaaac
    40981 atggtcatcg agctgacggc ggccagcgat tttaataaat tcatcggtgt tctaatccag
    41041 acccacgggc aaaacgcctg caaggcaggc gctgaaagag gcgaatagtc tacctggatt
    41101 atggtgaatt gctaccgcca gatgttacag ggtttattca ctcatcgcat cgcgccagat
    41161 cttgtcaacg atacgttgcg ccagcacggc ctgttcgccg gcggtttgcg gaactgtctg
    41221 attttgcaca cattcaatga agtgccgcgc acagccgaca aatccacgtt gttcaagagt
    41281 gctctgccaa ccaggaatcg gtttatgcac tacgccctgc ccgcgctcct cacgccattc
    41341 gcgcatatcc gtaatgtcga tgagcgcacc gtcagtcacg gcctgcacgg tttcacgctg
    41401 acttccggca cggcgatgca tacaggtggt gatctgcaaa ggaccggccg aaaaatggtg
    41461 ctcggcaaac agcatttcgc cagcgtcgtt agtcagtagc gtaccgccat ccagagaggc
    41521 tttgccgccc gacaaccaca gcgcggtatc caccacatgc agataatcat ccagcaacgt
    41581 gaaataaaga tcgtgtggcc cgacactatt gctacgatgt ttatccattc ttagcgaagc
    41641 tgcggtagcg agttgcgttt ttaactcacc gtagagtggt gcgaaacgac ggttaaaacc
    41701 gaccatcaac gtcagttttt tacgcgccgc cagttccacc agccgttcag catcgcgcag
    41761 attttctgcc agcggtttat cgacacagac atgtacaccc gcattgagta acgtactgac
    41821 cacgtcaaag tggctggcgg tgctggaatg cacaaaaacc gcatcgcaac tggcggcaag
    41881 gctggataac gaatcggcat aaggaatgcg ccagctttca caaattggca gggctttcgc
    41941 gcgcgtaggc gaccaggctc cttgtaacgt ccagtcagac gctgccgcca gcaccggtaa
    42001 ccacgctttt tgcgcaatgc cacctaatcc cactacgccg atacgtaatt ttttcacttt
    42061 aatctcccag atgggccagc aacgaatcaa gacgctgttt cagttctgcc acttcgattt
    42121 ccagggcttc gacgcgggcc tgtaaatcac catcaaccgc gttcgacata tccgtcaccg
    42181 ccggctgatc ttcaacctca ccactgaaaa gatgcatgta gcggctttcg cgtttaccca
    42241 gttcgcgggc cagacgcacc acaaaaggac catcttcgcg atttgccagt tgttccagcg
    42301 tcgactccac ttccgccata tcgctgaatt catacattcg cgcggcgcag ctgcgcagtt
    42361 cacctggcgt ctgggcacca cgcaataaca acgtggtgat taacgccact tccgctgcgc
    42421 tcagtttcag atcgccaaat tctgaattac aaaaacgttg ctcatatttg gtgacccgat
    42481 taccaaaacc gctcactgtg cgtagataat gacgtttgac cagattatcc agctgttcct
    42541 gcacttcgga ttcactcaga ttcatgaccg gttcacggtt cgttttctga ttacaggccg
    42601 tgactacacc attgactgag agcgggtatt gctccggcgt ggtcacctgc ttttccagca
    42661 agcaaccaat cacgcgagct tcaagggcag taagttgata tttcatgaga ttctccttag
    42721 cggccgggcg tccagtctgg ggtagttaat gccgtcagta cgtgatcgcg ccattgtcca
    42781 tcaatcaaca gatagtcttt cgcatagcct tctttttcaa aacccagtcg cgccagtaaa
    42841 tcaccgctgc gtttattgtg cggcatataa ttagccataa tgcgatgaat atgttgggtg
    42901 cgctgcatat aacgaatggc tgcggtcagg gcttcaaaca tgagtccttt gccctgccat
    42961 ttttgcccaa tcgaataacc gagatagcag gcatgaaaag agccacgaac aacattggaa
    43021 aaattggcaa cgccaataat ctctttttcg tccgggtcga ataagccaaa gtagaaagct
    43081 gaaccttgtt tatgaaattc gttaatcatc cccagcctgg cctgccagcc tgatggataa
    43141 cagtggcttt cgtcgcgcac tggctcccag ggcttgagga aatggcgatt ctctgcgtaa
    43201 taatccgcaa gacgccaggc atcacgatca tgcaccagac gcacgaccag tcggtctgtg
    43261 gttaagcgca cttttggcac gttactgcga tagccaaaca ttctatacct actccttccc
    43321 gtaacgtctt taatacgctg taagtattac tatacctgcg caaccagacc ctgtgaaaag
    43381 tccaaaatgc cattttctgc attattcaca tatttgataa aaactctcta tcaaagcgac
    43441 gttttgataa aaaaatattg tccccgaagt gatgggaaaa gtcttagcgg caatgcagaa
    43501 tagaaagtgc tcatctttta tttcccggga ggggaaatgt cccgcgtatc gcaggcgagg
    43561 aacctgggta aatatttcct gctcatcgat aatatgctgg tcgtgctggg gttctttgtt
    43621 gtcttcccgc tgatctctat ccgcttcgtt gatcaaatgg gctgggccgc cgtcatggtc
    43681 ggtattgctc tcggtctacg ccagtttatt cagcaaggtc tgggtatttt cggcggcgca
    43741 attgccgacc gctttggtgc caaaccgatg attgttaccg gtatgctgat gcgcgccgcc
    43801 ggattcgcca caatgggtat cgcccacgaa ccgtggctat tgtggttttc atgcctgctc
    43861 tcgggactcg gcggcacgtt gtttgatccg ccgcgttcgg cgctggtggt gaaactaatc
    43921 cgtccacaac aacgtggtcg ttttttctcg ctgttgatga tgcaggacag tgccggtgcg
    43981 gtcattggcg cattgttggg gagctggctg ttgcaatacg actttcgcct ggtctgcgcc
    44041 acaggggccg ttttgtttgt gctgtgtgcg gcgttcaatg cgtggttgtt accggcatgg
    44101 aaactctcca ccgtacacac gcccgttcgc gaaggcatga cccgcgtgat gcgtgacaag
    44161 cgttttgtca cctatgtcct gacgctggcg ggttactaca tgctggctgt acaagtgatg
    44221 ctgatgctgc caattatggt caacgacgtg gctggcgcac cctctgccgt taaatggatg
    44281 tatgccattg aagcgtgtct gtcgttaacg ttgctctacc ctatcgcccg ctggagtgaa
    44341 aagcattttc gtctggaaca ccggttgatg gctgggctgt tgataatgtc attaagcatg
    44401 atgccggtgg gcatggtcag cggcctgcaa caacttttca ccctgatttg tctgttttat
    44461 atcgggtcga tcattgccga gcctgcgcgt gaaaccttaa gtgcttcgct ggcggacgca
    44521 agagctcgcg gcagctatat ggggtttagc cgtctgggtc tggcgattgg cggcgctatt
    44581 ggttatatcg gtggtggctg gctgtttgac ctgggcaaat cggcgcacca gccagagctt
    44641 ccgtggatga tgctgggaat tattggcatc ttcactttcc ttgcgctggg ttggcagttt
    44701 agtcagaaac gcgccgcgcg tcgtttgctt gaacgcgacg cctgatttga tactcccccc
    44761 tgactcctcc atactgaaag tgaactgaca gccaaatgga ggagtcacgt gaagctatac
    44821 atttacgatc actgccctta ctgcctcaaa gcccgcatga ttttcggcct gaaaaatatc
    44881 cccgtcgaat tacatgttct gctcaacgac gacgcagaaa cacccacccg gatggtcggt
    44941 caaaaacagg ttcccattct gcaaaaagat gacagccgct atatgccaga aagcatggac
    45001 atcgttcact atgtcgataa actcgacggc aaaccgttac tgaccggcaa acgttcccct
    45061 gccattgaag agtggctgcg caaggtcaat ggctacgcaa acaaactgct gttgccgcgt
    45121 tttgccaaat cggcatttga tgagttttct actcccgccg cgcgcaaata tttcgtcgac
    45181 aagaaagagg ccagcgcggg taatttagcc gacctgctgg cccactctga cggtctgatt
    45241 aagaatatca gcgatgattt gcgtgcgctg gacaaactga tcgtcaaacc gaacgccgtg
    45301 aatggcgaac tttcggaaga tgatattcag ctatttccgc tactgcgtaa tctgacgctg
    45361 gtagccggaa tcaactggcc aagccgcgtt gctgattacc gcgataatat ggcgaaacag
    45421 acacaaatca atttgttatc atcaatggcg atttaatcct gacccgccgc ggcagcctgt
    45481 attcgcgggt tgccgccgtt ttcagttttt tctgctggcg agtttatcgc tggtgacaca
    45541 tgctgcgttg tgtcaggata ttgacgcaga tttcatgtcg aggactacca tgaacaagtt
    45601 tttatttgcc gctgcattga tcgtcagtgg cctgctcgtt ggctgtaatc aactcaccca
    45661 atacaccatc accgaacaag aaattaacca gtcgcttgcg aaacataata atttctcaaa
    45721 agatatcggt ttacccggcg tggctgacgc ccatattgtt ctgacaaact tgaccagcca
    45781 aattggtcgc gaagagccga ataaggttac cctaaccgga gacgccaatc tggacatgaa
    45841 ctccctgttc ggtagtcaga aagcgaccat gaaactgaag ctgaaagcgc tgcccgtgtt
    45901 tgataaagaa aaaggtgcga tcttcctgaa agagatggaa gtggtcgatg cgacggtaca
    45961 accggaaaaa atgcaaacgg tgatgcaaac gttgcttccc tatttgaacc aggcattacg
    46021 caattacttt aaccagcaac ctgcttacgt cctgcgcgaa gatggcagtc agggcgaagc
    46081 aatggcgaaa aaactggcga aaggcattga agtgaagcca ggcgaaattg tcattccgtt
    46141 tacggattaa tgacgagggc gcattcgcgc cctttatttt tcgtgcaaag gaaaacgttt
    46201 ccgcttatcc tttgtgtccg gcaaaaacat cccttcagcc ggagcataga gattaatgac
    46261 tgcaccatcc caggtattaa agatccgccg cccagacgac tggcaccttc acctccgcga
    46321 tggcgacatg ttaaaaactg tcgtgccgta taccagcgaa atttatggac gggctattgt
    46381 aatgcccaat ctggctccgc ccgtgaccac tgttgaggct gccgtggcgt atcgccagcg
    46441 tattcttgat gccgtacctg ccgggcacaa tttcacccca ttgatgacct gttatttaac
    46501 agattcgctg gatcctaatg agctggagcg cggatttaac gaaggcgtgt tcaccgctgc
    46561 aaaactttat ccggcaaacg caaccactaa ctccagccac ggcgtgacgt caattgacgc
    46621 aatcatgccg gtacttgagc gcatggaaaa aatcggtatg ccgctactgg tgcatggtga
    46681 agtgacacat gcagatatcg acatttttga tcgtgaagcg cgctttatag aaagcgtgat
    46741 ggaacctctg cgccagcgcc tgactgcgct gaaagtcgtt tttgagcaca tcaccaccaa
    46801 agatgctgcc gactatgtcc gtgacggaaa tgaacggctg gctgccacca tcactccgca
    46861 gcatctgatg tttaaccgca accatatgct ggttggtggc gtgcgtccgc acctgtattg
    46921 tttacccatc ctcaaacgca atattcacca acaggcattg cgtgaactgg tcgccagcgg
    46981 ttttaatcga gtattcctcg gtacggattc tgcgccacat gcacgtcatc gcaaagagag
    47041 cagttgcggc tgcgcgggct gcttcaacgc cccaaccgcg ctgggcagtt acgctaccgt
    47101 ctttgaagag atgaatgctc tgcagcactt tgaagcattc tgttctgtaa acggcccgca
    47161 gttctatggc ttgccggtca acgacacatt catcgaactg gtacgtgaag agcaacaggt
    47221 tgctgaaagc atcgcactga ctgatgacac cctggtgcca ttcctcgccg gggaaacggt
    47281 acgctggtcc gttaaacaat aaaaatcgta gcttcctgtt gtcattaggt tatttt
//
LOCUS       DAZFXT010000021        47031 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.021, whole genome shotgun sequence.
ACCESSION   DAZFXT010000021 DAZFXT010000000
VERSION     DAZFXT010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47031
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.021"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            218..769
                     /gene="ygiL"
                     /locus_tag="ACHKW0_001333"
     CDS             218..769
                     /gene="ygiL"
                     /locus_tag="ACHKW0_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HFO7474199.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPSKDATPGNVYAQADYKIAYE"
     gene            829..3333
                     /locus_tag="ACHKW0_001334"
     CDS             829..3333
                     /locus_tag="ACHKW0_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7474200.1"
                     /translation="MYKKLKLTTISELIKNIYCSLSVLIIGCASAYAVEFNKDLIEAE
                     DRENVNLSQFETDGQLPVGKYSLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTL
                     LGFTDEIIEVAQQNLIDGCYPIEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPP
                     ELWDHGIAGAFLDYNLYASHYAPHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFN
                     NGKSQANNLDFPRIYLFRPIPAINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPP
                     DLRGYAPQITGVAQTNAKVTVSQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEE
                     DGQVTQWQVASNSIPYLTRKGQIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNV
                     SLYGGSVLTNRDYQSLAAGVGFNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSK
                     RFESTGSQLTFAGYRFSDKNFVTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLN
                     TYVSLARNTYWDASSNVNYSLSLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNI
                     SIPWGTSRTLSYGMQRNQDNKISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRA
                     SYQHNTENGRLYLSGTSQRDSYYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDAD
                     GAEDIPLNNKRAVTNRYGIGVIPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEG
                     AIGYAKLDTRKGYQIMGVIRLADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQD
                     DSKLTLRWGDKSCFIQPPNSSNLTTGTVILPCISQN"
     gene            3349..4098
                     /gene="yqiH"
                     /locus_tag="ACHKW0_001335"
     CDS             3349..4098
                     /gene="yqiH"
                     /locus_tag="ACHKW0_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7474201.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCQTVNNSCNVSVAKRDK"
     gene            4100..5164
                     /gene="yqiI"
                     /locus_tag="ACHKW0_001336"
     CDS             4100..5164
                     /gene="yqiI"
                     /locus_tag="ACHKW0_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7474202.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(5207..5407)
                     /gene="glgS"
                     /locus_tag="ACHKW0_001337"
     CDS             complement(5207..5407)
                     /gene="glgS"
                     /locus_tag="ACHKW0_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HFO7474203.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            5676..6305
                     /gene="yqiJ"
                     /locus_tag="ACHKW0_001338"
     CDS             5676..6305
                     /gene="yqiJ"
                     /locus_tag="ACHKW0_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7474204.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            6332..7993
                     /gene="yqiK"
                     /locus_tag="ACHKW0_001339"
     CDS             6332..7993
                     /gene="yqiK"
                     /locus_tag="ACHKW0_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7474205.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            8232..8291
                     /locus_tag="ACHKW0_001340"
     CDS             8232..8291
                     /locus_tag="ACHKW0_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HFO7474206.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            8608..8667
                     /gene="ibsE"
                     /locus_tag="ACHKW0_001341"
     CDS             8608..8667
                     /gene="ibsE"
                     /locus_tag="ACHKW0_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HFO7474207.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(8787..10220)
                     /gene="hldE"
                     /locus_tag="ACHKW0_001342"
     CDS             complement(8787..10220)
                     /gene="hldE"
                     /locus_tag="ACHKW0_001342"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HFO7474208.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALVAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(10268..13108)
                     /gene="glnE"
                     /locus_tag="ACHKW0_001343"
     CDS             complement(10268..13108)
                     /gene="glnE"
                     /locus_tag="ACHKW0_001343"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HFO7474209.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(13131..14432)
                     /gene="ygiF"
                     /locus_tag="ACHKW0_001344"
     CDS             complement(13131..14432)
                     /gene="ygiF"
                     /locus_tag="ACHKW0_001344"
                     /EC_number="3.6.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HFO7474210.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            14674..15294
                     /gene="ygiM"
                     /locus_tag="ACHKW0_001345"
     CDS             14674..15294
                     /gene="ygiM"
                     /locus_tag="ACHKW0_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04211 family SH3 domain-containing protein"
                     /protein_id="HFO7474211.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            15358..16596
                     /gene="cca"
                     /locus_tag="ACHKW0_001346"
     CDS             15358..16596
                     /gene="cca"
                     /locus_tag="ACHKW0_001346"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HFO7474212.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(16759..17580)
                     /gene="bacA"
                     /locus_tag="ACHKW0_001347"
     CDS             complement(16759..17580)
                     /gene="bacA"
                     /locus_tag="ACHKW0_001347"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7474213.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(17671..18039)
                     /gene="folB"
                     /locus_tag="ACHKW0_001348"
     CDS             complement(17671..18039)
                     /gene="folB"
                     /locus_tag="ACHKW0_001348"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HFO7474214.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            18144..18761
                     /gene="plsY"
                     /locus_tag="ACHKW0_001349"
     CDS             18144..18761
                     /gene="plsY"
                     /locus_tag="ACHKW0_001349"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HFO7474215.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(18774..19706)
                     /gene="ttdR"
                     /locus_tag="ACHKW0_001350"
                     /gene_synonym="dan"
     CDS             complement(18774..19706)
                     /gene="ttdR"
                     /locus_tag="ACHKW0_001350"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate utilization transcriptional activator
                     TtdR"
                     /protein_id="HFO7474216.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVQVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            19913..20824
                     /gene="ttdA"
                     /locus_tag="ACHKW0_001351"
     CDS             19913..20824
                     /gene="ttdA"
                     /locus_tag="ACHKW0_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008730 - L(+)-tartrate dehydratase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HFO7474217.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            20821..21426
                     /gene="ttdB"
                     /locus_tag="ACHKW0_001352"
     CDS             20821..21426
                     /gene="ttdB"
                     /locus_tag="ACHKW0_001352"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HFO7474218.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            21475..22938
                     /gene="ttdT"
                     /locus_tag="ACHKW0_001353"
     CDS             21475..22938
                     /gene="ttdT"
                     /locus_tag="ACHKW0_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HFO7474219.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTVDYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(22981..23994)
                     /gene="tsaD"
                     /locus_tag="ACHKW0_001354"
     CDS             complement(22981..23994)
                     /gene="tsaD"
                     /locus_tag="ACHKW0_001354"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HFO7474220.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVTAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            24232..24447
                     /gene="rpsU"
                     /locus_tag="ACHKW0_001355"
     CDS             24232..24447
                     /gene="rpsU"
                     /locus_tag="ACHKW0_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HFO7474221.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            24558..26303
                     /gene="dnaG"
                     /locus_tag="ACHKW0_001356"
     CDS             24558..26303
                     /gene="dnaG"
                     /locus_tag="ACHKW0_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HFO7474222.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            26498..28339
                     /gene="rpoD"
                     /locus_tag="ACHKW0_001357"
     CDS             26498..28339
                     /gene="rpoD"
                     /locus_tag="ACHKW0_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HFO7474223.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(28418..28924)
                     /gene="mug"
                     /locus_tag="ACHKW0_001358"
     CDS             complement(28418..28924)
                     /gene="mug"
                     /locus_tag="ACHKW0_001358"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000700 - mismatch base pair DNA
                     N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HFO7474224.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            29049..29124
                     /locus_tag="ACHKW0_001359"
     tRNA            29049..29124
                     /locus_tag="ACHKW0_001359"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29082..29084,aa:Ile,seq:cat)
     gene            complement(29178..29942)
                     /gene="nfeF"
                     /locus_tag="ACHKW0_001360"
                     /gene_synonym="yqjH"
     CDS             complement(29178..29942)
                     /gene="nfeF"
                     /locus_tag="ACHKW0_001360"
                     /gene_synonym="yqjH"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase NfeF"
                     /protein_id="HFO7474225.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            30219..30842
                     /gene="nfeR"
                     /locus_tag="ACHKW0_001361"
                     /gene_synonym="yqjI"
     CDS             30219..30842
                     /gene="nfeR"
                     /locus_tag="ACHKW0_001361"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HFO7474226.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLHVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(30996..32516)
                     /gene="aer"
                     /locus_tag="ACHKW0_001362"
     CDS             complement(30996..32516)
                     /gene="aer"
                     /locus_tag="ACHKW0_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HFO7474227.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNSSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            32934..34313
                     /gene="ygjG"
                     /locus_tag="ACHKW0_001363"
     CDS             32934..34313
                     /gene="ygjG"
                     /locus_tag="ACHKW0_001363"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0033094 - putrescine--2-oxoglutarate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009447 - putrescine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HFO7474228.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(34355..34687)
                     /gene="ygjH"
                     /locus_tag="ACHKW0_001364"
     CDS             complement(34355..34687)
                     /gene="ygjH"
                     /locus_tag="ACHKW0_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HFO7474229.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            34906..35889
                     /gene="ebgR"
                     /locus_tag="ACHKW0_001365"
     CDS             34906..35889
                     /gene="ebgR"
                     /locus_tag="ACHKW0_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HFO7474230.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            36073..39165
                     /gene="ebgA"
                     /locus_tag="ACHKW0_001366"
     CDS             36073..39165
                     /gene="ebgA"
                     /locus_tag="ACHKW0_001366"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HFO7474231.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTHGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRGFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWRYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            39162..39611
                     /gene="ebgC"
                     /locus_tag="ACHKW0_001367"
     CDS             39162..39611
                     /gene="ebgC"
                     /locus_tag="ACHKW0_001367"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HFO7474232.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            39674..41107
                     /gene="ygjI"
                     /locus_tag="ACHKW0_001368"
     CDS             39674..41107
                     /gene="ygjI"
                     /locus_tag="ACHKW0_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7474233.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            41241..42311
                     /gene="ygjJ"
                     /locus_tag="ACHKW0_001369"
     CDS             41241..42311
                     /gene="ygjJ"
                     /locus_tag="ACHKW0_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HFO7474234.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            42328..44679
                     /gene="ygjK"
                     /locus_tag="ACHKW0_001370"
     CDS             42328..44679
                     /gene="ygjK"
                     /locus_tag="ACHKW0_001370"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HFO7474235.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGNKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            45005..47023
                     /gene="fadH"
                     /locus_tag="ACHKW0_001371"
     CDS             45005..47023
                     /gene="fadH"
                     /locus_tag="ACHKW0_001371"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HFO7474236.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFTWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
BASE COUNT        11743 a        12394 c        11792 g        11102 t
ORIGIN      
        1 ttatcgctta attccattca gaatatcatc acaaaaatga atgagtgcaa ttgaaaatat
       61 aattgttatg tattttttaa gttttactta atccatattt agataaacta atttcataaa
      121 acccgaaaag taaaatataa tatttgaatg attagcaatc cttaaataac agttactaat
      181 cattcatttt ctacttatat ttatataaag gaatattatg tctgctttta aaaagtcact
      241 tcttgttgct ggcgtagcaa tgattctttc caataacgta tttgctgacg aaggtcacgg
      301 cattgttaaa tttaaaggtg aagttatctc tgctccatgt tcaattaaac caggtgacga
      361 agacttaact gtaaatctgg gtgaagtagc agataccgtt ctgaaaagcg accagaaatc
      421 tttggctgag cctttcacta ttcacctgca agactgcatg ttgagccagg gtggaaccac
      481 ttattcaaaa gcgaaggtta ctttcaccac agcgaatact atgacgggtc agactgacct
      541 gctgaaaaac accaaagaaa ctgaaatcgg tggtgccaca ggcgttggcg tacgtattct
      601 ggatagccag agcggcgaag tgactttagg tactccggta gtaattacat tcaacaatac
      661 taacagctat caggaactga acttcaaagc tcgtatggaa tccccaagca aagatgctac
      721 cccgggcaac gtttacgctc aggctgacta taagattgct tacgagtaat cacattaggc
      781 cgggttcgcc cggccacatc attaacggat taatgataag tggaacagat gtataaaaaa
      841 ttaaaattaa ccacaataag cgaattgatt aaaaatattt attgttcatt atccgttctc
      901 atcattggtt gtgcgtcagc ttatgccgtt gaattcaaca aagatttaat cgaagccgaa
      961 gatcgtgaaa acgttaacct ttcccaattt gaaactgatg gccaattacc cgtaggtaaa
     1021 tactcattaa acgctcttat taataataaa aggacgccaa tccatctgga cctgcaatgg
     1081 gtattaattg ataaccaaac tgcagtttgt ctgactccag agcaattaac attattaggc
     1141 tttactgatg agattattga agtggctcag caaaacctga tcgatggttg ttaccctatc
     1201 gaaaaagaaa aacaaattac aacttatctc gataaaggga aaatgcaatt atccatatct
     1261 gcacctcagg catggttaaa atacaaagat gcaaactgga cgcctcctga actttgggat
     1321 catggtattg ctggggcatt tcttgactac aatttatatg cctctcatta tgcaccacat
     1381 cagggcgata attcacaaaa tataagttcc tatgggcagg ctggggttaa tcttggggcc
     1441 tggcgcctgc gtactgatta ccagtacgat cagtcattta acaatggcaa aagccaggcg
     1501 aacaacctgg attttccgcg tatttatttg tttcgcccaa tcccagcaat taatgcaaaa
     1561 ctaactatag gtcaatacga tactgaatcc tctattttcg actctttcca tttttctggc
     1621 gtttcgttga aaagcgatga gaatatgttg ccgcctgatc tacgtggtta cgcaccgcaa
     1681 atcacgggtg ttgcacaaac gaatgcaaag gtcactgtct cacagaacaa ccgtattatt
     1741 tatcaagaaa atgttcctcc tggcccattt gctattacta atttattcaa tacattacag
     1801 gggcaactgg acgtcaaggt tgaagaagag gacgggcaag tcacgcaatg gcaagttgca
     1861 tctaatagta ttccttatct gacgcgtaaa gggcagattc gctacaccac tgctatgggt
     1921 aaaccgacca gcgttggtgg tgattcctta caacaaccct tcttctggac tggtgaattc
     1981 tcatggggtt ggctgaacaa tgtatccctg tatggtggtt cagttttaac aaaccgtgat
     2041 tatcaatctc tggctgccgg cgttggcttt aaccttaact cattgggttc attatctttt
     2101 gatgtcacac gatctgatgc tcagttgcat aatcaggata aagaaacggg ttatagctac
     2161 cgcgctaact attcaaaacg ttttgaatct accggtagcc agctcacttt cgctggttac
     2221 cgtttctctg ataaaaactt tgtgacaatg aatgaatata tcaatgacac taaccattac
     2281 acgaattatc agaatgaaaa agagagttat attgtcacgt ttaaccagta tcttgaatca
     2341 ttaaggttaa atacatacgt aagtttggct cgtaatactt actgggacgc cagcagtaat
     2401 gtgaattatt cattatcact tagccgcgat tttgatatcg ggccattaaa aaacgtctcc
     2461 acttctctaa catttagccg aataaactgg gaagaagaca accaggatca actgtaccta
     2521 aatatttcga ttccctgggg aaccagtaga acattgagct atggtatgca acgaaatcag
     2581 gataacaaga tttcgcatac tgcttcgtgg tatgactctt ccgatcgaaa taattcctgg
     2641 agcgtttctg cttcaggcga caatgatgaa ttcaaagata tgaaagcgtc actacgcgcc
     2701 agttatcagc ataataccga gaacggtcgt ctctatctct ccgggacatc acagcgagac
     2761 agttattatt ctctgaatgc cagttggaat ggttcattca cggcgactcg tcacggagcc
     2821 gctttccatg actatagtgg tagtgctgac tcgcgtttta tgatcgacgc agatggtgca
     2881 gaagatattc cgttgaacaa taaacgcgcg gtaactaatc ggtatggcat cggagttatt
     2941 ccatcagtca gcagttacat aacaacatca ttaagcgttg atacccgaaa tcttccagaa
     3001 aatgtggata tcgaaaactc ggttatcacc accaccttaa ccgaaggtgc tattggctac
     3061 gccaaacttg atacccgcaa gggctatcaa atcatggggg ttattcgcct ggcagatggt
     3121 agtcacccac cactggggat tagcgtaaaa gataaaacca gccataaaga attaggactg
     3181 gttgctgatg gcggctttgt atacctcaac ggcattcagg atgacagcaa acttacttta
     3241 cgctggggtg ataaatcctg ttttattcaa ccacccaata gcagcaactt aaccaccgga
     3301 acggttattt taccgtgtat tagccaaaat taattcagga tgaaactaat gcgctactta
     3361 aacacaaaga atctcattgc agcaggagta ttactcgcct gcatgagcag cattgcctgg
     3421 ggtgccatca tcccggaccg tacccgtatc ataatgaatg agtcagataa aggggaagca
     3481 ttaaaactta ctaatcaaag caaaaatcta ccctatctgg cgcaaacatg gatagaagat
     3541 acgaaaggca ataaatcacg tgactttatt gtcacggttc cccctatggt acgtttaaat
     3601 cccagtgagc aaattcaaat aagaatgata agtcaggaaa aaattgctca actgcctaat
     3661 gacagagaaa cgctcttcta ttttaacgtg cgagaaatac cgccaaaaac agataaaaaa
     3721 aatgtgatgc aagtcactat gcagcacgca ttgaaactat tctggcgacc aaaagctatt
     3781 gaattagaag atgatggggt aatgtcttac gaaaaagttg aaatcatccg tagaaatgat
     3841 ggttcgatcc gctttaacaa taagatgcct tatcacgtta cgctgggtta tatcggaaca
     3901 aacggaatca caatgttgcc acaaacgcaa agtttaatgg taacccctta tagccatgca
     3961 gatacgcagt ttaaaaatgt tccgtcagct tttcaggttg gttatatcaa tgattttggt
     4021 ggattaagtt tttatgaaat aaactgccag acggttaata actcttgtaa tgtttccgta
     4081 gccaaacgag ataaataata tgcgctactt attaattgta attatattta ttataggttt
     4141 tagtgcatta cctgtatggg caatggactg ctatgctgaa catgaagggg gaaataccgt
     4201 agtcattggt tacgtaccaa gaatttctat ccccagtaat ggtaaaaaag gtgataagat
     4261 ctggcaaagc agtgagtatt ttatgaatgt cttctgtaat aatgcattac ctgccccatc
     4321 acctggagaa gaatacccat cagcctggac taatataatg atgtttttac caggcggtca
     4381 ggacttttat aatcagaact cctatatttt tggcgtaaca tacaatggag ttgattatga
     4441 ttcgactgcc cctaatgcta tcgcagcacc agaatgcatt gatataaaag gagcgggtac
     4501 ctttaataat cactataaaa accccgctgt ctgtagtgga ggtcctgagc ctcaattatc
     4561 agtaaccttt ccagctcgcg tacaactcta tattaaatta gcaaaaaatg ccaatagagt
     4621 taataaaaac ctggtgttac ctgatgaata tatcgctcta gagtttaaag gtatgagtgg
     4681 cgcaggagct atagaagtag acaaaaattt gaccttcagg attcgtggat taaataatat
     4741 tcatgtcctt gactgctttg ttaatgtcgc actggaacca gctgatggcg ttgttgactt
     4801 tggtaaaata aattcccgaa caattaaaaa taccagcgtt agtgagacgt ttagtgtagt
     4861 catgacaaaa gatcctggtg cggcctgtac tgagcaattc aatattttag ggagcttttt
     4921 cactacggat attttgagtg attatagcca tctggatatg ggtaatggac tgctattgaa
     4981 gatatttcat aaagatggaa cagcaacgga atttaaccgc ttctcacaat ttgcttcttt
     5041 ttcatcgtct agtgcacctt cggtcaccgc accattcagg gcagaactta gtgcgaatcc
     5101 ggcagagact gttgttgagg gaccgtttag taaagacgtc atcctcaaaa tcacctataa
     5161 ctagcatcta atacaaacac taaaacgggc catcaggccc gttatatcag tgctctaact
     5221 ccagctctct tgcctgcatc atctgttgac aataccaggc ataacgtgcg caaaaccagg
     5281 ttctatgttc ttcatccatg ttaccagtaa cggcaagaat atcgaccggg cgaccttctg
     5341 cgtgcattct ggcaaaacta cgcgccagga aatcgaaatt atttaaagaa ttaagactat
     5401 gatccataag cacatcctcc ggttatcgca taccttttaa ggccatatgc tttttctgat
     5461 ttcagtattc cccaatcagt gtagtgcctt taaacataac gtgcgtaaat atattgtccc
     5521 cgatcacact ttttagtgaa aaataaaaaa ccatattaaa aaactttctc ttccagaact
     5581 tctctaacat gtgttattat taacaaagat aaatgtaagc aaggaaacta tcccaaagta
     5641 aatattgcgt cactaaatgg acattggagt gacatatgat tttattcgcc gactataata
     5701 ccccttatct tttcgctatt tcctttgtac tgttgatcgg ccttttagaa attttagcgc
     5761 ttatctgcgg tcacatgctc tccggtgctc ttgatgcaca tcttgaccat tatgattcca
     5821 taaccacagg tcatattagc caggcacttc attatctcaa tatcggaaga ttgccagctc
     5881 tcgtcgttct ctgcttgctg gctggttttt ttggtcttat tggcatcctg ttgcagcatg
     5941 cctgcatcat ggtctggcaa tcaccactgt cgaacctgtt cgtggttcct gtcagtttac
     6001 tgtttacgat tattgcggtg cattataccg gtaaaatagt tgcgccctgg attccacgcg
     6061 accatagttc agctattaca gaagaagaat atattggtag catggcatta attaccggtc
     6121 atcaagccac ttcaggtaat ccctgcgaag gaaaactcac cgatcaattt ggccaaatac
     6181 attatttact actggagcct gaagaaggaa aatttttcac taaaggagac aaagtgttaa
     6241 ttatttgccg actttctgca acgcgatatt tggcggaaaa caatccctgg ccacaaatat
     6301 tgtaatttat taatcaaaag gaaatttaat aatggatgat attgttaatt ctgtgccctc
     6361 ctggatgttt accgcgatta ttgccgtatg cattctgttt attattggaa ttattttcgc
     6421 caggctctat cgtcgcgctt cggcagagca agcttttgtt cgtactggtt taggtgggca
     6481 aaaagtggtg atgagcggtg gcgcaatcgt gatgccgatc tttcatgaaa taatccccat
     6541 caatatgaat actctgaagc tggaagtcag ccgctcaacc attgatagcc tgattacgaa
     6601 agatcgtatg cgtgtcgatg tagtagtcgc tttctttgta cgggtaaaac cgtctgtaga
     6661 aggcatcgcc actgcggcgc agacgttggg gcaacgcacg ctatcaccag aagacttacg
     6721 tatgttggtt gaagataaat ttgtcgatgc cctccgtgca acagctgcac aaatgaccat
     6781 gcatgagtta caggataccc gcgagaactt cgtgcaggga gtacaaaata ctgtcgcaga
     6841 agacctgtcg aaaaacggtc ttgagctgga aagcgtttca cttaccaact ttaaccagac
     6901 ctcgaaagaa catttcaatc cgaacaatgc ctttgacgcc gaaggtttaa ccaaactgac
     6961 tcaggagacg gagcgccgtc gtcgcgaacg taacgaagtt gaacaggatg tagaagttgc
     7021 ggtgcgtgag aaaaaccgtg atgcactttc gcgcaagttg gagattgaac aacaagaagc
     7081 gtttatgacg cttgagcagg agcagcaggt taaaacccgt actgccgaac agaatgcacg
     7141 tattgcggct tttgaagctg aacgtcgtcg tgaagcagag cagacgcgaa ttctggctga
     7201 acgacaaatt caggaaacgg agatcgagcg cgagcaggcc gtccgctcaa gaaaggttga
     7261 ggctgaacgt gaagttcgta ttaaagagat cgaacaacag caggtcaccg aaatcgccaa
     7321 ccagacgaaa tcgatcgcta ttgccgccaa atcggaacaa cagtcccagg cagaagcgcg
     7381 tgctaacctt gcactcgcag aagcagtaag tgcgcaacaa aacgtagaaa ccactcgcca
     7441 gaccgcagaa gccgatcgtg ctaaacaagt tgccctaatc gctgccgcgc aggatgcaga
     7501 aaccaaagcg gttgaactga ccgtgcgggc gaaagcagaa aaagaagccg cagagatgca
     7561 ggcggcggct atcgttgagt tagccgaagc tacacgtaaa aagggtctgg cggaagcaga
     7621 agcacaacgt gcgctgaacg atgctatcaa cgtactttct gatgaacaaa ccagccttaa
     7681 attcaaactg gccttgttgc aggcgctacc tgcggtaata gaaaaatccg ttgagccgat
     7741 gaaatctatt gacggtatca agattattca ggtcgatggt ctgaatcgtg gcagcgctgc
     7801 gggtgatgca aacacgggta atgtgggggg cggaaacctg gcggaacaag cattatcagc
     7861 cgctctctct taccgcacac aggcaccgct gattgactcc ttgctcaatg aaattggcgt
     7921 ttcaggcggc tcactggcgg cattgacttc acccttaacc tcaacaactc ccgtcgccga
     7981 aaacgtagaa taaattttca gctatgaacg tccctggcac aaaaagccgg ggatttttta
     8041 tatctgcgtt ccgctaaaaa ctgcaaatga tcaggtcgtt gcaacgtttt gcgtgaccgc
     8101 tcggggaagg caaaactgcc tctgggaaag cgttgcgcga ggtccggcgc tcatcaacaa
     8161 tcggggagtc agcaaagggc tgaaacggga aagcccctcc cgaggaaggg gccttacaga
     8221 aggaaagggt tatgatgaag ctcgtcatca tactgattgt gttgttactc gtaagtttcg
     8281 cagcttatta acagccaatc agagggggga gaaatcctcc ctcacccttg ttcctttact
     8341 ttaggttgaa aaaaaatcag cgtcaagaag gtttcattaa cacatatccc tggcccaaaa
     8401 agccggggat tttttatatc tgcgttccgc taaaaggtgc aaatgctcag gacgttgcag
     8461 cgttttgcgt gaccgctcgg ggaaggcaaa actgcttcag ggaaagcgtt gcgcggggtc
     8521 cggcgctcat caacaatcgg ggagtcagca aggggctgaa acgggaaagc ccctcccgag
     8581 gaaggggcct tatagaagga aagggttatg atgaagttcg tcatcatact ggttgtgttg
     8641 ttactgttaa gtttcccgac ttactaacaa ctgatcagag gggggagaaa tcctccctta
     8701 cccttgttcc tttactttag gttgaaaaaa caacagcgtc aataggcctg cctgctacga
     8761 agcgagatct gtgaaccgct ttccggttag ccttttttat cctgttggat cttcttgatg
     8821 atgttagtgg tcgagcaacc gtcttcgaag ttgagcacca gcacttcgcc gccgttggcc
     8881 cagacttctt tactcccggc aatttcttct ggtttatagt cgccgccttt caccagcaga
     8941 tctggcagga tcccggcgat cagacgctgc ggcgtgtctt cttcaaacga cactacccag
     9001 tcgaccgcta ccagtgcgcc cagcacaatc atacgctgct cgagtgggtt taccgggcgg
     9061 gaatcccctt tcagccgttt ggtggaggca tcgctgttca cagcgacaat cagacggtca
     9121 cccagtttgc gagcatttgc cagataggag acgtgcccgg cgtgcaggat gtcaaagaca
     9181 ccgttggtca ttaccacctt ttcgccgcgt ttacgcgctg ccgctacagc aagcttcagt
     9241 tcctcttcgg tcatcacacc aaagccggta tctgcgcgcc cacgcactgc attttccagc
     9301 tcgatcggcg aaaccgtgga ggttcccagt ttgccgacca ccacgccagc cgccgcattg
     9361 gcaaagaagc aggcttcttc cagcgaatta cccgctgcca gcgttgccgc caggacgcca
     9421 atcaccgtgt cgcccgcacc ggtaacgtca tacacttcct gcgcctgggt tggcatatgc
     9481 agcggcgctt tacccggttg cagcagcgac ataccctgtt cggaacgggt cactaacaga
     9541 gccgagagtt cgtaatcggc aatcagtttc atgccgcgct caacaatctc ttcttcggtc
     9601 ttacatttac cgacaacagc ttcaaattcc gagagattcg gcgttaacag cgtagcgccg
     9661 cggtagcgct caaaatcggt accttttgga tcaatcagca ccggaacgcc cgctttacgc
     9721 gccagttgga tcatctgctg tacgcttgcc agcgcacctt tggcgtagtc agaaagcacc
     9781 agcgcgccaa tcgaactcag cgcctgatta atccgctcgt gcagcggctg cggatcaaca
     9841 ccttcgaaac cttcttcaaa atccaggcgg atcagctgtt ggttgcggga aagtacccgt
     9901 aatttggtga tagtcggatg cgtcggtaca gaaacgaagt cgcatttgac gttgacgtcg
     9961 gccagagatt tactcagcgc acgcgccgca tcgtcaatgc ccgtcagccc gaccaggcga
    10021 gcattggcac cgagagaagc gatgttcatt gccacgttag ccgcgccgcc cggacgttct
    10081 tcgatggtat tgactttaac cacgggcacc ggcgcttccg gcgagatacg gctggtggga
    10141 ccgtaccagt aacgatccag catcacatca ccaaccacca tcactcctgc acgttcaaac
    10201 tctggcagcg ttactttcat tcctgtctcc tgagagattc aaaatttgcg cgcgataata
    10261 ccatacttca ttcttccacc agccacttct gccagcttgc ccgcaccagt tcacgctctg
    10321 cggtgaagca atcctccggc acatggcccg gcaattcctg taatgccaga tgatgaagtt
    10381 catcgcgcag cgtagtataa gcacgggtca gcgccatcgc ttcctgctct tccataatgt
    10441 cgttttgcgc cagtagttcc agaatgcgca cgttgtctga ccagcgcgtt aacttcggtt
    10501 tttcatgagc gtagcgcaac accagatatt gggtaataaa ttcgatatcg gtaattccgc
    10561 cttcatcagc tttgatatca aagcgatcgc gatgtttatt gccgagatga gcgcgcattt
    10621 tctcgcgcat ttcccgcact tccgtttgca gagttttacc ttcacgcggc agcgtcataa
    10681 tctcgcgacg caccgcgtca aagtgcgcgg tgagctgcgg atcgccgtac actacccgcg
    10741 cacgcaccag cgcctgatgt tcccacgtcc aggcctcgtt tttctgataa tcggcaaatg
    10801 cttctgcgga tgtcaccagc attcccgcgg ccccggacgg gcgtagccgt gcatccactt
    10861 catacaaaat gccggaagag gtacgcgtac tgaacagatg cataatgcgt tgcgccagac
    10921 gcagataaaa ctgtcgcccg tcgatttccc gctcgccgtc agtcatcgca tccattgggc
    10981 aatcatggag gaagatcagg tcaagatcgg aactgtagcc taactcccag ccgcccagct
    11041 tgccgtagcc gaccaccgca aaaccacggc cttcgcgatc gttgaggtga tttggcttac
    11101 cgtagcgggc caccatttga acccacgcct gctgaacgac ggcatctatc atagcctctg
    11161 ccagccaggt taagtgatcg ctcactttca tcaccggtag cgtaccagcg atatccgctg
    11221 cggcgatgcg taacagctgc gcctgtttga actgacgcag cgcctcaagc tgttgctctt
    11281 cgtcatcttc cggcacgcgc agcaaatact ggcgcaactc atcgcggtag gcatcggtcg
    11341 ccgtcggctg gtaaagggtg tttggatcga gcaattcatc cagcaataat ggataacgcg
    11401 ccagttggct ggcaatcatc ggcgacgcgg cacacagaga aatcaaatgt ttaagcgccg
    11461 cggggaattc actgagcaat tctaaatagg tggtgcgggt aacaatcccc accagcaagg
    11521 cggtaatgcg cgacagcgta acggcagcgt cttcacgcgc acagacatca cttagcagat
    11581 gcggcatcag atggtcgagc acctgacgtc ctcgcggccc gatggtgcgc ttatccagct
    11641 ctttgcggaa atcggcaatc agcgttagca cctgtttgcg atcatcctcg ctaagatgcg
    11701 ccagcactgg cgtagtgtca tcttcctgca acgcatcctg ccacagctca cgccactgtt
    11761 ccgacagcga ctcttcctga gtttcacttt cgtcgtcgcc aatcaattca ttaaacaccc
    11821 ggcgcacatt ggtcatatgt gcggtcagcg ccccggtcag ttgcggccag tcagcaaagt
    11881 ccatcgccca cgccagccgc gcacggttaa gctcatcaga aggaagcgtc tgggtttgtt
    11941 cgtcgttaat gctttgcagc aggttttcca gacgccgcag gaacagatac gccactcgca
    12001 attgttcagc atcgttttca gaaagcagat gcagcgcggc aatgacgctg agcgttggca
    12061 gtaaagagcg cgactgcagc gacggttcgc gcccaccgcg aatgagctgg aacacctgaa
    12121 cgataaattc aatttcgcga atgccgcctg cgccgagttt gatattgtcg gtcaaaccac
    12181 gtcgacgcac ttcacgggca atcatccctt tcatgttgcg cagcgactga atcacgctga
    12241 aatcgatgta acgacggaaa acaaacgggc gcagcatcgc acgcaactcg ttagcataga
    12301 cgccttcgct atcgcccata atccgcgcct tgaccatcgc gtaacgctcc cagtcgcgcc
    12361 cctgctcctg gtaataatcc tccaacgcgg caaagctcag caccagcggg ccactttcgc
    12421 caaacggacg cagccgcata tccacgcgat agacgaagcc atcctgcgtt ggttgatcca
    12481 acactttaat cagccgctgc cccatgcggg taaaaaactg cgcgttatcc agttcccggc
    12541 gtccgccctg cgtacaacca tgttccggcc aggcaaaaat cagatcgata tctgaggaga
    12601 aattcagttc cccaccgccc agcttaccca tgcctaaaat cagcagcggt tgcgcttcgc
    12661 cctgcgcatt gcacggcgtt ccccactcgc ggcagcaagc gtcatacagc cagtcacgcg
    12721 cggcgacaat cagcgtctcc gccagatggc tgagctgctg caatatgctc tcttcagtaa
    12781 ccaaagccag cgtttgcgcc caggcgatgc gcaccataat gcgccgccgg aatagccgca
    12841 gctcgcgcat taacccggct tcgtcactca cattgctgag cgcctcctgc aaccatgccg
    12901 cgtaatgctg ccattcgtcg gcctgcggcg attggctttc cagttccgtc agccactctg
    12961 gatgcgcaat cacgctgtcc tgcacaaaat cactgaaagt aagtactgac ttcgcctgtg
    13021 cgctaagtga tttctcggct aaaggctctg gcagccgctc aacaacggtc tgccagtact
    13081 gctgtaacgg tgaagagagc ggcttcataa gcgattttat ccttgcctga ttaacgtttt
    13141 ccgctgtgca accagaacgg ttcctgattg tttgcctcat tacggaaatg ttcaatttca
    13201 atgcgttgcc cggttgcaat agcgtgacgc agcccctgcc agttctccag ccaggcttgc
    13261 gcgacgacag gatcatagta acccgccagc aacagtattg agtcaatatc acgcgtcaga
    13321 cgtggcaact ggtcacggta gcgatcgccc aacggctggc agaaaacgct tttcagttca
    13381 gcggcatggc gggaaagatg gatatcggca aagcgtttga aggaatcgct cattttactc
    13441 tgcgctttgg catctaaaaa cggctgccag gctttgctta ccagccattc ggtcaacgcc
    13501 agcttcgcca ttgccgtttt ggtagagtag accgccgtca cggcagaaac cgcagaagcg
    13561 atggtcgctt cgcattgagt cagcaaatca cgtaagtgag tgctcgcttt gcgcggcaca
    13621 ataccaccga acagcatcag tgtatgacgg accagaccaa tggctgccag cacctgttct
    13681 ttcgccgcat cgttgccgcg tacccacagt tcttcatgat actgccattg cgctaacgcc
    13741 agctcgagcg ccgcttccag cccctgttcc acatcggctt ttgccgcaac atgcaaaatg
    13801 gtggtcggtt tgatttcacg cgccggattg ccctgcgcca gatgatagcc acgcgccgct
    13861 ttgctcaggc tgccctggcg taatccggtt tgcgatacca gttggttcgc cagtttcagc
    13921 accgcgcgcg tgtcgccgct aagcagttcc agttccagct cacagatagg ttcagcaaat
    13981 tcccccgctt tcacctcccc ctgatcgaga gcgatttcaa ttcggctgtc atcgaccgcc
    14041 accagccatt tttcgcgata aaaatcggtg ctgaacagcg gttgtacgcg ggaagcgaga
    14101 tcggcgggca attcgccgtt cggccagact tccgtcggta actgcgccag gtcgagcgtc
    14161 ggttcgctca acgccacgtt atattccggg cgctggtgta agccgcctgt cactcttcct
    14221 gcaactttca tggtcatctc atagcgaccg ttttcgccac gaatacgtaa gcccatatcg
    14281 tgcccacgca gccagttatc cggcgtttcg tagtaaatat tcagcaactg cacggggtca
    14341 tggtgctcgc cgcccagcgt attgagatgg tcacgcaacg cctcaacggc actgtgatta
    14401 acaataaact ttaattcgat ttcctgagcc atggccttta cttatcggtt atgtcacata
    14461 agtgacgatg aacggcgaac ttaatgcgat cttttttgtc agtagatagt attttgcgcc
    14521 aaattgccat gcaacgggca atttgacggg cgtaaaagtt tgaagcagtg gcaagacctg
    14581 acacagagga ttccgattga tgtcgaatgc tttgcgtagc gacactgatg ccactactat
    14641 cgttccactt tccatgacaa taacgacagc ctgatgccaa aattacgcct gatcggatta
    14701 actttactcg cacttagcgc cactgccgtc tcacacgctg aagaaacgcg ctatgtttct
    14761 gatgaactga atacctgggt ccgtagcggt ccgggagatc attatcgcct cgtgggcacg
    14821 gttaacgccg gcgaggaagt gaccttatta caaactgacg ccaacaccaa ttatgcccag
    14881 gtgaaagaca gctctggccg taccgcctgg atcccgttga aacaacttag cactgagcca
    14941 agcctgcgct cccgtgtgcc agatctggaa aatcaggtca aaaccctgac cgataaactc
    15001 accaatatcg ataacacctg gaatcagcgc accgcagaaa tgcagcaaaa agtggcgcag
    15061 agcgacagcg tgatcaacgg attaaaagaa gaaaatcaga aattgaaaaa cgagctgatt
    15121 gtcgcgcaga aaaaggtcga tgccgccagc gtacagctgg atgacaaaca gcgcaccatc
    15181 atcatgcaat ggtttatgta tggcggtggc gtgctggggc ttggcttgct gctcggtctg
    15241 gtactgccac acctgatccc aacgcgcaaa cgcaaagatc gctggatgaa ctaaatcgcc
    15301 ttcttcgttg cactgtctta ttattaggaa ttgtgtattt gacgaaggga tgatggcgtg
    15361 aagatttatc tggtcggtgg tgctgttcgg gatgcattgt tagggctacc ggtcaaagac
    15421 agagattggg tggtggtcgg cagtacgcca caggagatgc tcgacgcggg ctaccagcag
    15481 gtaggccgcg attttcctgt gtttctgcat ccgcaaacgc atgaagagta tgcgctggca
    15541 cgtaccgaac ggaaatccgg ttccggttac accggtttta cttgctatgc cgcaccggat
    15601 gtcacgctgg aagatgatct taagcgtcgc gatctgacca ttaatgcgct ggctcaggac
    15661 gataacggcg agattatcga cccgtacaac ggtctgggcg atctgcaaaa tcgtctgttg
    15721 cgccatgttt cccccgcttt tggcgaagat ccgttacgcg ttctgcgcgt ggcgcgtttt
    15781 gctgcgcgtt atgcccacct cggttttcgt attgccgatg aaactctgac gctgatgcgc
    15841 gagatgaccc atgcgggtga actggaacac ctgacgcctg aacgggtatg gaaagagacg
    15901 gaaagcgccc tcaccacccg caatccccag gtgttcttcc aggtactgcg cgattgcggt
    15961 gcacttcgcg ttttattccc ggaaattgac gccctgtttg gcgttccggc ccctgccagg
    16021 tggcatccgg aaatcgacac gggtattcat accttaatga cgctctcaat ggcggcgatg
    16081 ctgagtccgc aggttgatgt ccgtttcgca actttatgcc acgatctcgg taaaggactg
    16141 acgccgccag aactttggcc gcgtcatcat gggcatggcc cggcgggtgt taagttagtg
    16201 gaacaactat gccagcgtct gcgcgtgcca aatgaaattc gcgatttagc cagactggtg
    16261 gctgagtttc acgatctcat ccacaccttc ccaatgctga acccaaaaac catcgtcaaa
    16321 ttatttgatt ccatcgatgc ctggcgtaaa ccgcagcgtg tcgagcaact ggcgctgacc
    16381 agcgaggctg acgtacgcgg cagaaccggt tttgaatcag cggactaccc gcaaggccgt
    16441 tggttgcgcg aagcctggga agtggcgcag tcagtgccga caaaagcggt cgttgaagcg
    16501 ggatttaaag gtgtggagat tcgcgaggag ctgacccgac ggcggattgc ggcggtagcc
    16561 agttggaagg aacaacgttg cccaaagcct gactgagtat aaaactaaac gttaacacga
    16621 accatcccct cgccccttcg gggagagggt tagggtgagg ggaacagacc agcaccggtg
    16681 cgaacaatca cccccacccc ggccctctcc ctacagagcg agggagaaaa ccaggcctgc
    16741 tcccgaacct tttctcaatc aaaagaacac gacgtacacc gcagccgcca caataaagcg
    16801 ataaatggcg aacgggataa acgaaatgcg cttaatcaat tgcaggaagg ttttaatcgc
    16861 tatcagcgcc accacaaaag cggtgataaa cccaacggca aacatcggga tatcgccggt
    16921 tgtcaggaag ccccagcttt tgtagagatc gagcgccgtt gcgcccatca tcatcggtac
    16981 cgccagcagg aacgagaact cggaagcagc gtaacggctc acgcccatca gcatcccacc
    17041 tgaaatggtg gccccggaac gggagaaacc cggccacagc gccagacact ggaaacagcc
    17101 aatcataaac gcctgacgat aggtcatatc atcaagaccc ggcgcacgcg gctctttcgg
    17161 cttcaggcat tcggcggcaa tcagcaacaa accgccaacg accagtgcat acatcacatt
    17221 tatcgggtta aacaatgact taatcgtgtc gtggaacaac agccccagta ccaccgccgg
    17281 aatcatcccc agcaaaatgt ggatcagcgt taaacgacct ttgctttctc cttcgtgctg
    17341 caacgggcgg ccaaagtgga tgccaatcag gccaaacaga cgccgccaga acatcactac
    17401 taccgccaga attgatccta actggatcac aacttcaaag gttttcgccg tgtcgccctc
    17461 gaaccccaac aagtgaccga caataatcat atggcccgtg ctggataccg gcagaaattc
    17521 tgtcaatcct tcgaccacac ccaatattgc cgctatcagc agcgagtgca tatcgctcat
    17581 caataaaccc ctaaattatt aaaatgtacc gcttgtccgg actactgcag taagaccagg
    17641 ttataaccgt ttggtttaac agctgtaaaa ttaattattt tctttcagat tattgccacg
    17701 ctcaatgatt acgccaacat tcgccgcccg cgccactgcg cctggcttgc tgagtttgat
    17761 acgcacccac ggcgagttga agcgtgctaa cagcagctcc gccacctctt cagccacgcg
    17821 ttccaccagc gcaaaacgcg ccccctcgac gtggctgacc accgtttctg caatgtcagc
    17881 gtaactgagg caatccgcca catcatcact tttcgccgct ttacggttat cccatgccat
    17941 ttcgatatcg aacactaact tctgttcgat ggtctgttcc cagtcgtaaa caccaatagt
    18001 ggtgattacc gaaagttgct ctataaatac aatatccatc acgtcctgcc tgctttttgg
    18061 ctaaccggat accacttccg gcgaaatgtg cgtattatcc acagattcat cgttgaacac
    18121 gaattttcaa aacggaacag cttatgagtg caatcgcgcc tggaatgatc ctcatcgcgt
    18181 acctctgcgg ctccatttcc agtgccattc tggtttgccg cttgtgtggg ctgcccgatc
    18241 cgcgaaccag cggctccggc aatccaggcg caaccaatgt gttacgtatc ggtggcaagg
    18301 gagcagccgt agcagtactg attttcgacg ttctgaaagg aatgttgccc gtctggggcg
    18361 cgtatgaatt aggtgtcagc cccttctggc taggcttaat tgccatcgcc gcctgtcttg
    18421 gacacatctg gcccgttttc ttcggattta aaggaggaaa aggcgttgct accgcttttg
    18481 gtgccatcgc acccattggc tgggatctca ccggagtaat ggcgggaacc tggttactga
    18541 ccgtgctatt gagcggatac tcgtcgctgg gagcgattgt cagtgcactg attgctccgt
    18601 tttatgtatg gtggtttaag ccacaattca ccttcccggt ttcgatgctc tcttgcctga
    18661 tcctgctgcg tcatcatgac aacatccagc gtctgtggcg tcgtcaggag acaaaaatct
    18721 ggacgaaatt caaaagaaag cgcgaaaagg atcccgagtg atttctggtg gatctacatg
    18781 acctgatagc cttcatcggg cttgcccagc cgttgctggc accatgccgc cagaaattcc
    18841 acgcagacac gtaatttcat gctgcgatag agcggctccc ggtaaacagc ccagatattg
    18901 gcgctttgtg catactccgg caatacttgc accagtttgc cactctccag aaacggcaac
    18961 acatcccact cggaacgcag cataatccct ttgccctcca gcgcccattg caacactatc
    19021 tcgccgctat tggaggaaag atgcccgcta accttcaccg attttttctc ctgaccgttc
    19081 cccaactccc atattccgtg ggtcatatcg cgctctttgg tcaccagaca gtcatgacga
    19141 cttaattcct gtaatgattg tggctgcgga tatttttgca gatactcagg tgctgcgcat
    19201 aatattcttt tatttttcgt taacagatgc gcaatataat aatcaggaat ttcgtcatta
    19261 atacgaatat ccagatcaat attatcctgc actaaatcaa tttgccgatc gaacaattca
    19321 aaatgcacct gtaactcagg ataattgcgc ataagttcgg taatggctgg cgcaatatgg
    19381 ctgcgcccaa aaccaaaact acagccaata cgaatcatcc cctccgggcg cgttttgatt
    19441 tgcgtgacgt catcgaccag tcgctgatat tgggtgagga tttccagcgc atgttcgtaa
    19501 caacgttggc cactttcagt cagcgccacg ccgcgagcgg agcggttcag cagcgttgtt
    19561 gccagcgtat tttcaaggat ttggatccgc ttggtgacaa atgcaggcgt ctgcccaagc
    19621 gtcgccgctg cggcgctaaa actcccgctg tggacgattt ctaccagtac ctgcaagtct
    19681 ttggctaaag gccagctatt cagcatcgtt aattatccgc agttgtgata agcgcagtgt
    19741 atttcgcaaa acattgccat tccctgctct ggcgcaatat tcattcttat tttcgcgttg
    19801 attcacgaac tgttaattac atattcacgg tggcaaaaaa tataaaacca catttttagt
    19861 ggtagtttgt ggcggtgaat ttttccagac aaatacaaaa actggagttg ccatgatgag
    19921 cgaaagtaat aagcaacagg cagtgaataa gttgacagag attgtcgcta actttaccgc
    19981 catgatttct acccgaatgc ctgatgacgt ggtggataaa ctaaaacagc taaaggatgc
    20041 cgaaacgtcg tcgatgggga aaattatcta ccatacgatg ttcgacaaca tgcaaaaagc
    20101 gattgacctg aatcgtcctg cctgtcagga caccggggag attatgttct tcgttaaagt
    20161 cggttcccgc ttcccactgc ttggcgagct gcaaagcata ctcaaacaag ccgtggaaga
    20221 ggcaaccgtc aaagcgccac tacgtcacaa tgcggtagaa atttttgacg aagtaaacac
    20281 cggcaaaaat accggtagcg gcgtaccgtg ggtcacctgg gacatcatcc ccgacaatga
    20341 cgatgcagaa atcgaagttt acatggcagg cggcggctgc acgctacctg gccgctcgaa
    20401 agtgttaatg ccgtcagaag gctacgaagg cgtggtgaaa ttcgtcttcg aaaatatctc
    20461 caccctcgcc gtaaacgcct gtccaccggt actggtgggc gtgggcatcg ccacctcggt
    20521 ggaaaccgcc gccgtactct cgcgtaaagc cattttgcgt ccgattggca gccgccatcc
    20581 caatccaaaa gcggcagagc tggagctacg cctggaagaa ggactcaacc gtctggggat
    20641 tggtccacaa gggctgaccg gcaacagttc agtgatgggc gtgcatatcg aatctgccgc
    20701 ccgccatccg tcaaccatcg gcgttgctgt ctctaccggc tgctgggcgc atcgtcgcgg
    20761 cacactgctg gttcatgccg atctcacctt tgaaaatctg tctcacaccc ggagcgcgtt
    20821 atgaaaaaga tcctgacaac cccgatcaaa gctgaagatc tgcaagatat tcgcgtcggc
    20881 gatgtgatct acctgaccgg tacgctggtg acctgccgtg acgtttgtca ccgccgcttg
    20941 atcgaactga aacgtccgat cccttacgat ctcaacggca aagcgatttt ccacgctggc
    21001 cccatcgtgc gcaaaaacgg cgataaatgg gagatggttt ccgttggccc gacaaccagt
    21061 atgcgtatgg aaagttttga acgtgaattt atcgagcaga ccggcgtgaa actggtggtt
    21121 ggcaaaggcg gaatggggcc gctgaccgaa gaaggctgcc agaaattcaa ggcgctacat
    21181 gtgattttcc cggcaggctg cgcggtgctg gcggcaaccc aggtggaaga gattgaagaa
    21241 gtgcactgga cagaactcgg gatgccggag tcactgtggg tctgccgggt caaagagttc
    21301 ggcccgctga ttgtctctat tgatacccac ggcaacaacc tgatagccga aaacaaaaag
    21361 ctgttcgccg aacgccgcga tcccatcgtg gaagagatct gcgagcacgt ccattacatc
    21421 aaataaccct cccggagagg ctcgccccct ctcctttttc gcaggcataa cacgatgaaa
    21481 ccttccactg aatggtggcg ataccttgcg ccgctggcgg tcatcgccat tattgctcta
    21541 attccggttc ccgcagggct ggagagtcat acctggctct actttgccgt ttttactggc
    21601 gtgatcgttg gactgatcct cgaacccgtg ccgggtgccg tggtggcgat ggtgggtatc
    21661 tccatcatcg ccatactctc tccctggctg ctgttcagcc ccgaacagct cgcccagcca
    21721 ggctttaaat tcactgcaaa atccctctcg tgggcggttt ccggtttttc taattcggtt
    21781 atctggctga ttttcgccgc ctttatgttt ggcacaggct atgaaaaaac cgggctggga
    21841 cgccgcattg cgctgattct ggtgaaaaag atgggacatc gcacgctgtt tctcggctat
    21901 gcggtgatgt tctccgagct gatcctcgca cctgtaacac cgtccaactc ggcgcgtggt
    21961 gcggggatta tctatcccat catccgtaac ctgccaccgc tctatcaatc acaaccaaac
    22021 gacagcagtt cgcgcagcat tggctcgtac atcatgtgga tggggattgt tgccgactgc
    22081 gtgaccagcg ccattttctt gacggcgatg gcaccaaact tgctgttaat tggactgatg
    22141 aaaagcgcat ctcacgccac gctgagttgg ggcgactggt tcctcgggat gttgccgctc
    22201 agtattttgc tggtgctgct ggttccctgg ctggcttatg tgctgtaccc gccggtactg
    22261 aagtcaggcg atcaggtgcc gcgctgggca gagacggaac tgcaggcaat gggcccgctc
    22321 tgttcgcgtg aaaaacggat gctggggctg atggtaggcg cgctggtgct gtggattttc
    22381 ggcggtgatt atatcgatgc cgcgatggtc ggttacagcg tggtggcact gatgctgctt
    22441 ctgcgcatta tcagttggga cgacattgtc agtaataaag ccgcgtggaa cgttttcttc
    22501 tggctggcct cgcttatcac cctcgctacc ggactcaaca acactggttt tattagctgg
    22561 tttggcaaac tgttagcagg cagcttaagc ggttattcgc caacgatggt gatggtggcg
    22621 ttgattgtgg tgttttatct gctgcgctac tttttcgcca gcgccacggc gtatacctcc
    22681 gccctcgcac cgatgatgat tgccgctgcg ctggcgatgc cggaaatccc gctgccggtg
    22741 ttctgcctga tggttggcgc ggcaattggt ctggggagca ttcttacacc atacgccacc
    22801 ggacccagtc cgatttacta cggtagtggt tatctgccaa cggtggatta ctggcgactg
    22861 ggggcgattt ttggactgat attcctcgtg ttgctggtga ttaccggctt gctgtggatg
    22921 cccgtggtgt tgctttaagt gaggacggtg ccggaaattg ctctccggca ccggatagtt
    22981 ttacgcagcc ggtaactctg ccagtggcca gcgcggacgc acgctaacgc cgagatccgc
    23041 cgtcgcgcct gctttaaacc gcaccattcc ggcataggcg atcatcgcgc cgttatcagt
    23101 acaaaattcc ggacgcgcgt agaacacttc gccgcggcgt tttttcatca tttccgccag
    23161 cttcgcccgt aacgtgcggt tagcactcac gccgcccgcc atgaccagtc gcttaaagcc
    23221 cgtctgatcc aacgctcgct tacacttaat catcaacgta tcgaccaccg catcttcaaa
    23281 ggcgcgggcg atatcagcac gcgtctggtc gtcggtgccg ttgtcacgaa tcgtgttcgc
    23341 cgcaaaggtt ttcagaccag aaaagctgaa atccagcccc ggacggtcgg tcatcggacg
    23401 cgggaaaaca aagcgcccgg cagtaccctg agccgccatt ttcgacagta acggtccgcc
    23461 aggataatcc agccccagca gcttcgcggt tttatcaaac gcttcaccgg cggcatcatc
    23521 gatagactcg ccgagcagct cgtactgacc aatgccagtc acgctgatta actgcgtatg
    23581 accgccggac accagcagcg cgacaaacgg aaattccggc gggttatctt ccagcatcgg
    23641 cgctaacaga tgcccttcca tatggtgtac cgggattgcc gtaacgttcc aggcaaacgc
    23701 cagagaacgc cccacggttg cgccaaccag cagtgcgccg actaatccag ggcctgcggt
    23761 ataggccaca gcatcaatgt cttttgccgt taaaccagac tcctttagcg ccgcctggat
    23821 caacggtacg gttttgcgca cgtgatcgcg ggaggccagt tcaggcacga cgccgccgta
    23881 gtcagcgtgc aatttcacct gactatacaa ttggttggct aacaaacctt tttcatcgtc
    23941 gtaaatggcg atgccggttt catcgcagga agtttcaata cccagtacac gcatgacttg
    24001 ttttacctcg ctttattacc gcgcagtgta ggaccaatgc gggttgatgt aaaactttgt
    24061 tcgcccctgg agaaagcctc gtgtatactc ctcaccctta taaaagtccc tttcaaaaaa
    24121 ggccgcggtg ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata
    24181 agctggcgtt gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta
    24241 attaaagtac gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc
    24301 gaaaaagcag gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc
    24361 gaacgtaagc gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa
    24421 aacgcacgcc gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga
    24481 gttgtagttg taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt
    24541 gctaaaaatc ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg
    24601 cacgcactga catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga
    24661 atttccacgc gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg
    24721 agaaacagtt ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga
    24781 tgaactacga caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc
    24841 ttgaagtgcc atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa
    24901 cgctttatca gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg
    24961 ttgccacgtc tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc
    25021 gctttgcgat tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca
    25081 atccagaaaa tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac
    25141 gcagttacga tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg
    25201 tgattggttt tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg
    25261 aaacagacat tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata
    25321 acgctgaacc caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc
    25381 aatacggcat taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac
    25441 aactgttgtt ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc
    25501 gcgatgccgc atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc
    25561 tacgctttat gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta
    25621 aagaagcgtt tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca
    25681 gcctgatgcc gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg
    25741 cactgccatt gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat
    25801 taggcaacaa attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg
    25861 cagagagcgg cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta
    25921 tagggttgct ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg
    25981 atgaaaataa gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc
    26041 agccaggtct gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca
    26101 cccttgaaaa actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct
    26161 tcaccgactc actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa
    26221 tcgctcgtga gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa
    26281 accaggagct ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc
    26341 cccgacagcc gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt
    26401 ggcggtaaac aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc
    26461 aacaccaacc tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca
    26521 gctgaaactt cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa
    26581 tgaccatctg ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat
    26641 caacgacatg ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc
    26701 tgaaaacacc gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga
    26761 atctgaaatc gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt
    26821 tgaactgttg acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa
    26881 ccaggttcaa tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta
    26941 cgatcgtgtt gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc
    27001 gaacgcagaa gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga
    27061 agatctggac gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga
    27121 tgacaacagc atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta
    27181 cgttgtaacg cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga
    27241 gatcctgaaa ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta
    27301 cctggtcaac agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat
    27361 gaagctctgc gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg
    27421 caacgaaacc agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga
    27481 aaaactgcac gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga
    27541 agaagaaacc ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg
    27601 tgaagcgaaa gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat
    27661 ttctatcgcc aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg
    27721 caacatcggt ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc
    27781 cacctacgca acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg
    27841 caccatccgt attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg
    27901 ccagatgctg caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct
    27961 gatgccggaa gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga
    28021 aacgccgatc ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct
    28081 cgagctgccg ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct
    28141 ggctggcctg accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa
    28201 caccgaccac acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg
    28261 tcagatcgaa gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg
    28321 tagcttcctg gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag
    28381 tccctttgca caaacgccac cttttcggtg gcgtttttta tcgcccacgc actaccagcg
    28441 cctggtccag ctcgcgatac gcttcaacca gtttctctag tgaaacgcga cttaaaccgc
    28501 tgggatttgg cagcacccaa atctgcgtcg aaccaatggt gagcgtttgt ttcccccact
    28561 gtgcaccgcg ctggctgaat ccctgttcat atgcttgttt gcccagaatc gccaacgcct
    28621 gcggctgata atcttcaatt ttttcaatca gcttacgccc acctgcgtgc agctcctgct
    28681 ttgaaacttc attggcttgc accgttggac ggtctaccag tttggtgacg ccacaacgat
    28741 aatccagcag atgctgtgcc tcctgcggct tcaactgacg gtcggtaaac ccggcctgat
    28801 atatcacctt ccagaagcga tttgctggat gagcaaaggg aaaaccagtc ccagcggatg
    28861 aaagcccagg gttgataccg caaaacacga cccgtaaccc tggagccaaa atatcctcaa
    28921 ccatatttac tcctttctaa catcaccagg aaaagtataa cggattgaaa atacattgtt
    28981 tataaaaaca gcaggcgcac ggtaatggct ggattgcgac acggagttac tttataatcc
    29041 gctaccatgg ccccttagct cagtggttag agcaggcgac tcataatcgc ttggtcgctg
    29101 gttcaagtcc agcaggggcc accagatata gcaaaggctg acgagaaatc gtcagccttt
    29161 ttctttttat atatcagtta ctttgcgtgc cagtaagccg ctgcacgtac ccgctgtggg
    29221 tcatactgtt ccgcttcaaa gcggcggctt aaattcttaa cgactttacc ttcgccggtt
    29281 atccagatga agtaatcatc ggcagggatt tgcatctgcg ccagacgcgc atctaccgcc
    29341 tgctcatcat gtgccagcca ttcgatatta aaaccatcaa ggtgcgcgag ataatcctga
    29401 caggcgttat cccgcacgct aaccagcgca ctaacttgcg gtttaacggc aagtttgctc
    29461 aacgtttcca ggcggcggcg cagtgcgggc attccggatt catcgcagac atacagctga
    29521 tacgcgtaat cttccggcac caccagcgaa ccgcgcggac ctgccaccgt aagtttatcg
    29581 cccggttgcg cctgcatcgc ccagccgctg gcgaccccac cgtcgtgaat aaagaaatcg
    29641 atcgccagtt catggcgtag ttcgtcatac agcggcgtat agtcacgcga cggtgggcgt
    29701 ggtccttccg gccagacgat gccctcttcc gttaccgttg gcggcacaaa gtgagcgtca
    29761 ggttgaggaa agaagagttt gctgtgatcg tcaaagccgc gcgatgtaaa accgtccagc
    29821 gcctcgccgc cgaggacaat gcgctgaaaa ccggcgctga tgcgctcaac gcgtaacaca
    29881 gtcagttcac ggaagcgcag atcattgcga acgcgctgcg ggtagcgggg ggagttattc
    29941 attgttatcg ccttcgtgat agtaatcaga tatatctaaa taattttcgt aaatgataat
    30001 gattgttaat catgataaat gcaagcgatt tgtagaactg atatatctga agtatgtgaa
    30061 ggcattcagt gccatagcaa tgctgccgga tgcggcgtga acgtcttatc cggcctggat
    30121 gtgaggtgct tccacgttta aacatcattt ttgtacttgc agtaatgctc acatcagata
    30181 taaattagat atatctaatc aagcaaaagg aggctgatat gagccatcat cacgaagggt
    30241 gttgtaaaca tgaaggccag ccacgccatg agggctgctg caaaggtgag aagtcagaac
    30301 acgagcactg cggacacggt caccagcatg aacacggtca atgctgcggt ggtcgccacg
    30361 gtcgcggcgg cggtcgtcgg caacgtttct ttggtcacgg tgaattgcgt ctggtgattc
    30421 tggatattct ctcgcgcgat gacagccacg gttacgaatt gattaaagcg attgagaacc
    30481 taacccaggg gaattacacc ccaagcccgg gcgtcatcta cccaacgctg gattttctgc
    30541 aggagcagtc gctgattacc atccgcgaag aggaaggtgg taagaagcag attgcgctga
    30601 ccgaacaagg cgctcagtgg ctggaagaaa accgcgaaca ggtggagatg attgaagaac
    30661 gcatcaaagc gcgttgcgtt ggcgcggcgc tgcgccagaa cccgcaaatg aagcgggcgc
    30721 tggataattt taaagcggtg ctggatttac acgtcaacca gagcgatatc agtgatgcac
    30781 aaataaaaaa gatcattgcg gtgatcgacc gcgccgcttt tgatattacg caactggatt
    30841 aatcgccgca cccgccagtg gcgcggtgca attgccggat gcgacgcttg acgcgcctta
    30901 tccggcctac acccgctaca caccccgcag gcctgataag atgcgccagc atcgcatcag
    30961 gcattgtgct ccaaccgccg gatccggcat accgattaat gcagcaccgt caccgcgtct
    31021 tccagtcggc tggcgcggtg tttcaccatc gccgacacct gcgcactctc ttccaccagc
    31081 tcggcatttt tctgggtgat caggttaagc tcatccactg cacgggtcag gctggaaagc
    31141 ccatcggcct gttccagcgt tgaatggcta atctgggcga tcaactgggt gacgtttttc
    31201 acctgtgcca caatatcttc catcgtccgt ccggcggcgt gtacctgctg cgaaccggat
    31261 tgcaccttat cagcactggc atcaatcagc ttgcgaatat cgttggcagc attagcgctg
    31321 cggctggcta aatgacgcac ttcccctgcc accactgcaa aacctttgcc ctgttcgccg
    31381 gcacgcgccg cttccaccgc cgcattcagg gccagaatat tggtctgaaa cgcaatatcg
    31441 ttaatcagcg aagtaatggt gccaatgcgc tgggtactgt cggcgatatc gtccattgtc
    31501 ttgatcaccg tggtcatcgc ctccccaccc tgcactgccg cattactggc ggtgatagaa
    31561 agtttatcgg cagccgacgc cgtggcagag ttctgtttca ccgacgccgc catttggttc
    31621 atggtcgcca ccgtttgctg aacgttatca actgtctgct gggtatgttc gttcagttca
    31681 tcggtgcctt tcgccagcgt ctcactgcta tttctgacac tggacacctg gcttgagaca
    31741 tcgttaatta gccaacggca catcaggcca agctgcccta ccgcgcgtaa tgtcagcccc
    31801 agctcatcgc tgcgattcag gtgctcaaca ctattacgct ctccggtcgc caccttcagt
    31861 gcctgacggg cgacattttc tatcgggcgc acaatctgcc attcaaaaca agcgcttgcc
    31921 aacaatacca ctaacacaca gaggaaatac gtcaccaccg gggcagcaac aaaccaaagc
    31981 atggccgcca gcaagataaa catcagggtc atcactccac gcgcccgcca gcgaagcggt
    32041 aatgaaggca gtttacccag ccagccttta cgcaccacca ggcctttatg aatacgctta
    32101 ctggtacgtc cggcgttcag cgctttgtac agcggctcca ccgccgcaat ctcttcatcc
    32161 gtcgcccggg tacgaatcga catatagcca ctgatttttc cctcgcgcac catcggtacc
    32221 gcattggccc gcacccaata atggtcacca tttttgcggc gatttttcac gatgccgctc
    32281 cagggctccc cttttttcag ggtgaaccac atatccgcaa acgccgcttt tggcatatcc
    32341 gggtgacgca ccatgttgtg cggctgccct tgtaactctt gcaaggtaaa gccgctcacc
    32401 tgcacaaaag tgtcattagc atgagtgata tagctttgca gatcggtagt ggacatcaga
    32461 gtggtatcgt ccgccagcgg ggtattttgc tgggtgacat acggatgaga agacatgttc
    32521 gcgtcctgtg caggttatat ggttgttaac ttcttgtcag agtttatgtc ggccccgctg
    32581 cggttatctt taaccgatta atttgattta gatcgcaatt tgcgattaaa gcacaaatct
    32641 aattctttga tttaaaatac tttcactctg ttactatacg aaaacgttaa ttatcttgcc
    32701 caaaaatcag gcaattattg ccctgaaaac gtgcatttgc gcagcaatca tcaaatccat
    32761 acccgacaaa aaccgtgcaa aataacaaca aatgttaaca gatagcatta aatattgcac
    32821 aaatgataac cgaatttgtg tttatcccga ttttcgcgat cgcagccgga gtggcgcaat
    32881 ccctgcaata cttaaatcgg tatcatgtga tacgcgagcc tccggagcat attttgaaca
    32941 ggttaccttc gagcgcatcg gctttagcgt gcagcgccca cgccctgaat ctcattgaga
    33001 agcgaacgct ggatcatgag gagatgaaag cacttaaccg agaggtgatt gaatacttca
    33061 aagagcatgt caatccgggg tttttagagt atcgcaaatc tgttaccgcc ggcggggatt
    33121 acggagccgt agagtggcaa gcgggaggtt taaatacgct tgtcgacacc cagggacagg
    33181 agtttatcga ctgcctggga ggttttggaa ttttcaacgt ggggcaccgt aatccagttg
    33241 tggtttccgc cgtacagaat caacttgcga aacaaccgct gcacagccag gagctgctcg
    33301 atccgttacg ggcgatgttg gcgaaaaccc ttgctgcgct aacgcccggt aaactgaaat
    33361 acagcttctt ctgtaatagc ggcaccgagt ccgttgaagc agcgctgaag ctggcgaaag
    33421 cttaccagtc accgcgcggc aagtttactt ttattgccac cagcggcgcg ttccacggta
    33481 aatcacttgg cgcgctgtcg gccacggcga aatcgacctt ccgcaaaccg tttatgccac
    33541 tgctgccggg cttccgtcat gtgccgtttg gcaatatcga agccatgcgc acggctctta
    33601 acgagtgcaa aaaaaccggt gatgatgtgg ctgcggtgat cctcgaaccg attcagggtg
    33661 aaggtggcgt aattctgccg ccgccgggct atctcaccgc cgtacgtaag ctatgcgatg
    33721 agttcggcgc actgatgatc ctcgatgaag tacaaacggg catggggcgc acgggcaaga
    33781 tgttcgcctg cgagcatgag aacgtacagc cggatatcct ctgccttgcc aaagcgctcg
    33841 gcggcggcgt gatgccgatt ggcgcgacca tcgccactga agaggtgttt tcagttctgt
    33901 tcgacaaccc attcctgcat accaccacct ttggcggcaa cccgctggcc tgtgcggcgg
    33961 cgctggcgac catcaatgtg ttgctggagc agaacttacc ggctcaggct gagcaaaaag
    34021 gcgatatgtt gctggacggt ttccgtcaac tggcgcggga atatcccgat ctggtacagg
    34081 aagcgcgtgg taaagggatg ttgatggcaa ttgagtttgt tgataacgaa atcggctata
    34141 actttgccag cgagatgttc cgccagcgcg tactggtggc cggaacgctc aataacgcca
    34201 aaacgatccg cattgaaccg ccactgacac tgaccattga acagtgtgaa ctggtgatca
    34261 aagcggcgcg taaggcgctg gcggccatgc gagtaagtgt cgaagaagcg taatatcaaa
    34321 tcggatggcg atacgacgtc gccatccgat ctttttatac gacgcgtacg cccgcaggca
    34381 tcatccgctc cggcgttaac aacacgcttt cactgccgtc atcggtttcc gcacacaaca
    34441 gcatgcattc cgacgtttca ccacgcattt tcgctttttg cagattgcat aagaccacca
    34501 ccgttttccc catcagctct tcttcgctgt agtacggcac caggctggtc acggtttgca
    34561 gtgttttttc ccccacatca acctgtacga tgtacagctt gtcggcgttt tcatggcgtt
    34621 tcacttccac aatctttccg acgcgcattt ccagacgtgc aaaatcagcg taagccacgg
    34681 tttccattgt ttcctccctt cgggtaaaat tttactaaac tatagcaaaa gtttttctca
    34741 atcctgtagg ctaaaaatgg agaatgcagg cgtgatcaca ttcgtgagcc gctgtgttac
    34801 cgttacagcg tcaaagaaac gcgctttatt tactgaaaac aggtgacccg ataagcactt
    34861 cctctacaat gggggcgcac atcagggaaa gtaaaaaagg taaacatggc aacactaaaa
    34921 gacatcgcaa tcgaagctgg cgtatccctg gcgacagtat ccagggtctt aaatgacgat
    34981 ccgacattga atgtgaaaga agagacgaaa catcgcattc tcgagatcgc cgaaaagctg
    35041 gagtacaaga ccagtagtgc ccgtaaactc cagacaggtg cagtcaacca acaccatatt
    35101 ctggctatct acagctacca acaggagctg gagatcaacg atccttacta tctggcgatc
    35161 cgccacggca ttgaaactca gtgcgaaaag ctgggcattg agctcaccaa ctgttatgaa
    35221 cacagcggat taccagacat taaaaacgtc accggtattt taattgtcgg caaacccacg
    35281 cccgccctgc gcgccgccgc ctgtgcgttg accgacaata tctgttttat cgactttcac
    35341 gaacccggca gcggttacga tgcggtggat atcgatctgg cacgcatcag taaagaaatc
    35401 atcgacttct atatcaacca gggcgttaat cgtattggtt ttattggcgg tgaagatgag
    35461 cctggcaagg cggatattcg tgaggtcgcc tttgcggaat atggccgact gaaacaagtg
    35521 gtacgcgaag aggatatctg gcgcggcggt ttttccagtt cgtcgggtta tgaactggca
    35581 aaacaaatgc tggcgcggga agactatccg aaggcactgt ttgttgcttc cgattccatt
    35641 gctatcggcg tactgcgggc aattcatgaa cgaggcctga acatcccaca ggatatttcg
    35701 cttatcagcg ttaacgatat ccccaccgcg cgatttacct ttccgccgct ctccaccgtg
    35761 cgcatccatt ccgagatgat gggaagtcag ggcgttaacc tggtgtatga aaaagcccgc
    35821 gatggtcgcg cgctgccgct gttagtcttc gttcccagca aattaaaact gcgcggcacg
    35881 acccgttaaa tccccttaca cactgtccgg caatcgtttt tgccggacag tgctgccgtt
    35941 tattttcgtg atccagttaa agtaaatgca tttacctgct actttttagt aaaaatttta
    36001 ctaaactccc cagcaattac acaaactacc atcaccatga atggttccga tttctctcta
    36061 ccgggaggcc ctatgaatcg ctgggaaaac attcagctca cccacgaaaa ccgacttgcg
    36121 ccgcgtgcgt actttttttc atatgattct gttgcgcaag cgcgtacctt tgcccgcgaa
    36181 accagcagcc tgtttctgcc cttaagcggt cagtggaatt tccacttttt tgaccatccg
    36241 ctgcaagtac cagaagcctt cacctctgag ttaatggctg actgggggca tattaccgtc
    36301 cccgccatgt ggcaaatgga aggtcacggc aaactgcaat ataccgacga aggttttccg
    36361 ttccccatcg atgtgccgtt tgtccccagc gataacccaa ccggtgccta tcaacgtatt
    36421 ttcaccctca gcgacggctg gcagggtaaa cagacgctga ttaaatttga cggcgtcgaa
    36481 acctattttg aagtctatgt taacggtcag tatgtgggtt tcagcaaggg cagtcgcctg
    36541 accgcagagt ttgacatcag cgcgatggtt aaaaccggcg acaacctgtt gtgtgtgcgc
    36601 gtgatgcagt gggcggactc tacctacgtg gaagaccagg atatgtggtg gtcagcgggg
    36661 atcttccgcg atgtttatct ggtcggaaaa cacctaacgc atattaacga tttcactgtg
    36721 cgtaccgact ttgacgaagc ctattgcgat gccacgcttt cctgcgaagt ggtgctggaa
    36781 aatctcgccg cctcccctgt cgtcacgacg ctggaatata ccctgtttga tggcgaacgc
    36841 gtggtgcaca gcagcgccat tgatcatttg gcaattgaaa aactgaccag cgccagcttt
    36901 gcttttactg tcgaacagcc gcagcaatgg tcagcagaat ccccttatct ttaccatctg
    36961 gtcatgacgc tgaaagacgc caacggcaac gttctggaag tggtgccaca acgcgttggc
    37021 ttccgtgata tcaaagtgcg cgacggtctg ttctggatca ataaccgtta tgtgatgctg
    37081 cacggcgtca accgtcacga caacgatcat cgcaaaggcc gcgccgttgg aatggatcgc
    37141 gtcgagaaag atctccagtt gatgaagcag cacaatatca actccgtgcg taccgctcac
    37201 tacccgaacg atccgcgttt ttacgaactg tgtgatatct acggcctgtt tgtgatggcg
    37261 gaaaccgacg tcgaatcgca cggctttgct aatgtcggcg atatcagccg tattaccgac
    37321 gatccgcagt gggaaaaagt ctacgtcgag cgcattgttc gccatattca cgcgcagaaa
    37381 aaccatccgt cgatcatcat ctggtcgctg ggcaatgaat ccggctatgg ctgtaacatc
    37441 cgcgcgatgt accacgcagc gaaggcgctg gatgacacgc gactggtgca ttacgaagaa
    37501 gatcgcgatg ctgaagtggt cgatattatt tccaccatgt acacccgcgt gccgctgatg
    37561 aatgagtttg gtgaataccc gcatccgaag ccgcgcatca tctgtgaata tgctcatgcg
    37621 atggggaacg gaccaggcgg gctgacggag taccagaacg tcttctataa gcacgattgc
    37681 attcagggac attatgtctg ggaatggtgc gaccacggga tccaggcgca ggatgacaac
    37741 ggcaacgtct ggtataaatt cggcggcgac tacggcgact atcccaacaa ctataacttc
    37801 tgtcttgatg gtttgatcta ttccgatcag acgccgggac cgggcctgaa agagtacaaa
    37861 caggttatcg cgccggtaaa aatccacgcg ctggatctga ctcacggcga gctgaaagtc
    37921 gaaaataaac tgtggtttac cacgcttgat gactacaccc tgcacgcaga ggtgcgcgtc
    37981 gaaggtgaaa cgctcgcgac gcagcagatt aaactgcgcg acgttgcgcc gaacagcgaa
    38041 gcccccttgc agatcacgct gccgcagctg gacgcccgcg aagcgttcct caacattacg
    38101 gtgaccaaag attcccgcac ccgctacagc gaagccgggc attctatcgc cacttatcag
    38161 ttcccgctga aggaaaacac cgcgcaacca gtgcctttcg caccaaataa tgcgcgtccg
    38221 ctgacgctgg aagacgatcg tttgagctgc accgttcgcg gctacaactt cgcgatcacc
    38281 ttctcaaaaa tgagtggcaa accgacatcc tggcaggtga atggcgagtc gctgctgacc
    38341 cgcgagccaa agatcaactt cttcaagcca atgatcgaca accacaagca ggagtacgaa
    38401 gggctgtggc aaccgaatca tttgcagatc atgcaggaac atctgcgcgg ctttgccgta
    38461 gaacagagcg atggtgaagt gttgatcatc agccgcacag ttattgcccc gccggtgttt
    38521 gacttcggga tgcgctgcac ctacatctgg cgcatcgctg ccgatggcca ggttaacgtg
    38581 gcgctttccg gcgagcgtta cggcgactat ccgcacatca ttccgtgcat cggtttcacc
    38641 atggggatta acggcgaata cgatcaggtg gcgtattacg gtcgtggacc gggcgaaaac
    38701 tacgccgaca gccagcaggc taacatcatc gatatctggc gcagcaccgt cgatgccatg
    38761 ttcgagaact atcccttccc gcagaacaac ggcaaccgtc agcatgtccg ctggacggca
    38821 ctgactaacc gccacggcaa cggtctgctg gtggttccgc agcgcccaat taacttcagc
    38881 gcctggcgct atacccagga aaacatccac gctgcccagc actgtaacga gctgcagcgc
    38941 agtgatgaca tcaccctgaa cctcgaccac cagctgcttg gcctcggctc caactcctgg
    39001 ggcagcgagg tgctggactc ctggcgcgtc tggttccgtg acttcagcta cggctttacg
    39061 ttgctgccgg tttctggcgg agaagctacc gcgcaaagcc tggcgtcgta tgagttcggc
    39121 gcagggttct tttccacgaa tttgcacagc gagaataagc aatgaggatc atcgataact
    39181 tagaacagtt ccgccagatt tacgcctctg gcaagaagtg gcaacgctgc gttgaagcga
    39241 ttgaaaatat cgacaacatt cagcctggcg tcgcccactc catcggtgac tcattgactt
    39301 accgcgtgga gacagactcc gcgaccgatg cgctatttac cgggcatcga cgctattttg
    39361 aagtgcatta ctacctgcaa gggcagcaaa aaattgaata tgcgccgaaa gagacattac
    39421 aggtagtgga atattatcgt gatgaaactg accgtgaata tttaaaaggc tgcggagaaa
    39481 ccgttgaggt ccacgaaggg caaatcgtta tttgcgatat ccatgaagcg tatcggttta
    39541 tctgcaataa cgcggtcaaa aaagtggttc tcaaagtcac catcgaagat ggttatttcc
    39601 ataacaaata acaactacgg cggcaaaagg agtttgccgc caccgctacc ctacacattt
    39661 tcggagatgt gttatgtctg ataccaaacg taatacaatc ggcaaattcg gcttgctctc
    39721 gctgactttt gccgccgttt acagctttaa caacgttatc aataataata ttgagcttgg
    39781 actggcctcg gcaccgatgt ttttcctcgc gacgattttt tattttattc ccttctgtct
    39841 gatcatcgca gaatttgttt cgttaaataa aaactcagaa gccggtgtct acgcgtgggt
    39901 aaaaagttcg ctgggcggac gttgggcatt tattactgcc tatacctact ggttcgtaaa
    39961 cctgttcttt ttcacctcac tgttgccgcg cgttattgct tatgcttcgt atgccttcct
    40021 cggctacgaa tatattatga cgccggttgc caccaccatt atcagtatgg tgctgttcgc
    40081 cttctccacc tgggtttcca ccaacggggc gaaaatgttg gggccaatta cctccgtcac
    40141 ttcaacgctg atgctgctgt taacgctctc ctacatttta ctggcaggta cggcgctggt
    40201 tggcggcgta cagcctgctg accccatcac cgttgacgcg atgatcccga acttcaactg
    40261 ggcgttcctc ggcgttacca cctggatctt tatggccgca ggtggcgcgg agtccgtcgc
    40321 tgtgtacgtt aacgacgtca aaggcggttc gaaatcgttc gttaaagtga tcatcctcgc
    40381 cgggattttt atcggcgtac tgtattccgt ctcctcggtg ctgattaacg tcttcgtcag
    40441 cagcaaagag ttgaaattta ccggcggatc ggtgcaggta ttccacggca tggcggcgta
    40501 ttttggtcta ccggaagcgt tgatgaatcg ctttgtcggt ctggtgtcct ttaccgcgat
    40561 gttcggttcc ctgctgatgt ggaccgcaac gccggtgaaa attttcttct ccgaaatccc
    40621 ggaaggcatc tttggtaaga aaaccgtcga actgaacgaa aacggcgttc cagcgcgcgc
    40681 agcgtggatc cagttcctga tcgtcatccc gctgatgatt atcccgatgc tcggttccaa
    40741 taccgtgcag gatctgatga atactattat taatatgact gccgcagcgt ccatgcttcc
    40801 gccgttattc atcatgctgg cttacctgaa tttacgcgcc aaattagatc acctgccacg
    40861 cgatttccgc atgggctccc gccgcaccgg tattatcgtt gtttcaatgc tgattgcgat
    40921 atttgccgta gggtttgtcg cttcgacatt cccgactggc gcgaatattc tgaccatcat
    40981 tttttataac gtcggcggta ttgttatctt cctcggcttt gcgtggtgga aatacagtaa
    41041 atatataaag ggattaacgg ctgaagagcg ccatattgaa gcgacgccag ccagcaatgt
    41101 tgattgagca gactaataaa aaatgagcag gcattttccc tcttcctgga tggggagggg
    41161 gaaaatagtt tctgccttta tatttttata attacaacga taaaaggctg tactttttct
    41221 ttagctcatg gattaacaca atgaaattaa tcactgcacc atgcagagca ttacttgctc
    41281 tgccgttttg ctacgccttt tctgcggcag gagaagaagc acgtccggca gaacatgacg
    41341 acacaaaaac acctgcaatt acctcgacat cttctccttc atttcgtttt tacggcgaat
    41401 taggggttgg tggatatatg gatttagagg gtgagaataa acataaatac agcgacggta
    41461 cctatattga aggtggcctg gagatgaagt acggctcctg gttcggcctg atttacggcg
    41521 aaggctggac cgtgcaggcc gaccacgacg gcaatgcctg ggtgccagac catagctggg
    41581 gtggtttcga gggcggaatt aaccgtttct atggcggtta tcgtaccaat gatggcaccg
    41641 aaatcatgct cagtctgcgt caggattcct cgctggatga cctgcaatgg tggggcgact
    41701 tcacccccga tctgggctac gtcattccca atacccgcga cattatgacc gcgctgaagg
    41761 tgcagaactt aagcggcaac tttcgttata gcgtcaccgc cacacctgcc ggacatcatg
    41821 acgaaagcaa agcctggcta cattttggca agtacgatcg ctatgacgac aaatacacct
    41881 atccggcaat gatgaacggt tacatccagt atgaccttgc cgaaggcatc acctggatga
    41941 acggtctgga aatcaccgac ggcacaggac agctctatct cacgggcctg ctaactccta
    42001 actttgccgc tcgcgcctgg caccataccg gacgcgccga cgggctggac gtaccgggaa
    42061 gtgaaagtgg gatgatggtg agcgccatgt atgaagcgtt aaagggcgtt tatctctcca
    42121 ccgcttacac ctacgccaaa catcgccctg accacgctga cgatgaaacc acctctttca
    42181 tgcagtttgg tatctggtac gaatacggcg gcggacgttt cgccacggct tttgatagcc
    42241 gcttctacat gaaaaatgcc tctcacgatc ccagcgacca aatcttcctg atgcaatatt
    42301 tctactggta ataaggactg taatttcatg aaaataaaaa ctattttaac gccagtaacc
    42361 tgcgctctgc tgataagttt ttccgcccat gccgctaacg ccgacaatta taaaaacgtg
    42421 attaaccgta ctggcgcgcc gcagtacatg aaggattacg attacgacga tcaccagcgt
    42481 tttaatccgt ttttcgatct cggagcctgg catggtcatc tgttgccaga cggccctaac
    42541 accatgggcg gctttccggg cgttgcgctg ctgacggaag agtacatcaa ctttatggcc
    42601 agcaatttcg accgcctgac cgtctggcag gacggcaaga aagtcgactt tacgctggag
    42661 gcatacagta ttcccggtgc gctggtgcaa aaactgacag caaaagatgt gcaagtcgaa
    42721 atgactctgc gcttcgccac gccgcgcacg tcactactgg aaaccaaaat caccagcaat
    42781 aaaccgctgg atctggtgtg ggacggcgaa ctgctggaaa aactggaagc gaaagaaggg
    42841 aaaccgcttt ccgataaaac cattgctggc gaataccctg actatcagcg caaaatcagc
    42901 gccacccgtg atggcctgaa agtcaccttt ggcaaagtgc gcgccacctg ggatctgctg
    42961 acctccggcg aatcggaata tcaggtgcat aaatccctgc cggtgcagac tgaaatcaac
    43021 ggtaatcgct ttaccagtaa ggcgcatatc aacggttcga ccacgctcta caccacctat
    43081 tcccatctgc tgaccgctca ggaagttagc aaagagcaaa tgcagatccg cgatattctg
    43141 gcgcgtccgg cgttttatct caccgcctcg cagcaacgct gggaagaata cctgaagaaa
    43201 gggttaacca atccggatgc gacgccggaa cagacgcgcg tcgcggtgaa agcgatcgaa
    43261 acgctcaacg gtaactggcg ctcaccgggc ggtgcggtga aatttaacac cgttacaccg
    43321 tcggtgaccg ggcgctggtt ctccggcaat cagacctggc cgtgggatac ctggaagcag
    43381 gcgtttgcga tggcgcattt caatcccgac atcgccaaag agaatatccg cgcggtcttc
    43441 tcctggcaga tccagcctgg cgatagcgtg cgtccgcagg atgtgggctt tgtccccgac
    43501 ctgatagcgt ggaaccttag ccccgagcgt ggtggcgatg gcggcaactg gaacgaacgt
    43561 aataccaaac ccagccttgc cgcctggtcg gtgatggaag tgtacaacgt cacccaggat
    43621 aaagcctggt tggcagagat gtacccgaaa ctggtggcct atcacgactg gtggttacgt
    43681 aaccgcgatc acaacggcaa cggcgtgccg gaatatggcg cgacccgcga caaagcccac
    43741 aacactgaga gcggcgagat gctgtttacg gtgaagaaag gcaacaaaga agagacgcag
    43801 tctgggctga acaactacgc ccgcgtggtg gagaaaggcc agtacgacag tctggaaatt
    43861 ccggcacagg tcgctgcgtc gtgggaatcg gggcgtgatg atgccgccgt ctttggtttt
    43921 atcgacaaag aacagttgga taaatatgtc gctaatggcg gcaaacgtag cgactggacg
    43981 gtgaaattcg ccgaaaaccg cagtcaggac ggaacgttgc tgggctactc gctattgcag
    44041 gagtcggtgg atcaggccag ctatatgtac agcgataacc attatctggc ggagatggca
    44101 accatcctcg gtaagccaga agaggccaaa cgctatcgcc agttggcaca gcagctcgcg
    44161 gactacatca acacctgtat gttcgacccg actacccagt tctactatga cgtacgtatt
    44221 gaagataaac cactggcgaa cggctgcgcg ggcaaaccga ttgttgagcg cggtaaaggg
    44281 ccggaaggct ggtcgccgct gtttaacggt gcggcaacgc aggccaatgc cgacgcggtg
    44341 gtgaaggtga tgctcgatcc taaagagttc aacacctttg tcccgctggg aacggcggcg
    44401 ttaaccaatc cggcttttgg cgctgatatc tactggcgtg ggcgcgtatg ggtggatcag
    44461 ttctggtttg gtctgaaagg gatggagcgt tacggttatc gcgatgatgc cctgaaactg
    44521 gcggatacgt tcttccgtca cgccaaagga ttaaccgccg atggcccaat tcaggagaat
    44581 tacaacccgc tgactggcgc acagcaaggc gcaccaaatt tctcctggag tgccgcgcat
    44641 ttgtatatgt tgtataacga ttttttccgt aagcaataat ttgcaactgc tggcggatgc
    44701 ggcgtaaacg ccttatccgc cctacatgtg caatcctcct ggccggataa aacgcgacaa
    44761 gcgtcgcatc cggcatctgc accgtgccac tggcggatgc ggcgtgaacg ccttatccgc
    44821 cctacatgtg caatccccca ggccggataa aacgcggcaa gcgtcgcatc cggcgttacc
    44881 accgggcgta ttctttttga atcccatcac aaaccccgca ctcccctttt cccttttctc
    44941 cggcgacggc taaattagaa ctcatccgac cacataacaa ttattttaca tactggacaa
    45001 ttttatgagc tacccgtcgc tgttcgcccc gctggattta ggttttacca cgttaaaaaa
    45061 ccgcgtgttg atgggctcaa tgcacaccgg gctggaggaa tacccggacg gtgccgagcg
    45121 gctggcagcg ttttatgccg aacgcgcccg tcacggcgtg gcgctgattg tcagcggcgg
    45181 cattgcacca gatttaacag gcgttggcat ggaaggtggc gcaatgctca acgacgccag
    45241 ccagatccca caccatcgca ccattaccga agcggtacat caggaaggcg gcaaaatagc
    45301 cctgcaaatt ttgcataccg ggcgctacag ctaccaaccg catctggtcg ctccctccgc
    45361 attgcaggcc cccatcaacc gtttcgttcc tcacgaactc acccatgaag agatcctgca
    45421 actgatcgac gatttcgccc actgcgcgca actggcacgg gaggcaggat acgacggtgt
    45481 agaggtaatg gggtccgaag ggtatttgat caacgaattt ctgacgctgc gcaccaatca
    45541 gcgtagtgac cagtggggcg gcgattaccg caaccggatg cgatttgccg tagaagtagt
    45601 gcgtgcggtg cgcgaacgcg tcggcaacga cttcattatt atctaccgac tgtcgatgct
    45661 cgacctggtc gaagacggcg ggacttttgc cgaaacggta gagctggcgc aggccattga
    45721 agcggcgggc gcgaccatta tcaacaccgg cattggctgg catgaagcac gtattccgac
    45781 cattgccacg cccgtgccgc gcggcgcatt tacttgggtc acgcgcaaac tgaaaggcca
    45841 cgtttcgctg ccgctggtga ccaccaaccg gattaacgat ccgcaggttg ccgacgacat
    45901 tctctcgcgc ggcgatgccg atatggtgtc gatggcacga ccgtttcttg ctgatgcgga
    45961 actgctgtca aaagcgcaat cggggcgagc cgatgagatc aacacctgca ttggctgcaa
    46021 tcaggcctgc ctcgatcaga tcttcgttgg caaagtcacc tcgtgcctgg tgaatcctcg
    46081 cgcctgccac gaaaccaaaa tgccaatcct tcctgccgtg cagaaaaaaa atctggcggt
    46141 ggtcggtgcg ggacctgctg ggctggcgtt tgccattaac gcggcggcgc gtgggcatca
    46201 ggtaacattg tttgatgccc acagcgagat cggcgggcag tttaatatcg ccaaacagat
    46261 ccccggcaaa gaggagtttt acgaaacgct gcgctattac caccggatga tcgaagtgac
    46321 gggcgtgacg ctgaaactca atcacaccgt gacggcggat cagttacagg cgttcgatga
    46381 aacgatcctc gccagtggga tcgtgccgcg cgttccgcct atcgacggga tcgatcatcc
    46441 gaaggtattg agttatctcg atgtactgcg cgacaaagcg ccggtaggca acaaggttgc
    46501 catcatcggt tgtggcggga ttggttttga tacggcgatg tatttaagtc agccgggcga
    46561 atccaccagc cagaatatcg ccaggttctg taatgagtgg gggatcgaca gcagcctaca
    46621 acaggctggt ggcttaagcc cgcagggaat gcagatcccg cgtagcccac ggcaaattgt
    46681 gatgctccag cgcaaagcca gcaaaccggg acaggggtta ggcaaaacca ccggctggat
    46741 ccatcgcacc accctgctct cgcggggtgt gaaaatgatc ccaggcgtaa gttatcagaa
    46801 gattgacgat gacgggctgc atgtggtgat caacggcgaa acgcaggtat tagcagtgga
    46861 caatgtggtg atctgcgcag ggcaagagcc aaaccgcgcg ctggcgcaac cgctgattga
    46921 tagcgggaaa actgtgcatt taattggcgg ctgcgatgtg gctatggagc tggacgcacg
    46981 acgggcaatt gcccagggaa cacggctggc gctggagatt taaatcgtta t
//
LOCUS       DAZFXT010000022        45145 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.022, whole genome shotgun sequence.
ACCESSION   DAZFXT010000022 DAZFXT010000000
VERSION     DAZFXT010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45145
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.022"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(17..547)
                     /gene="ydeS"
                     /locus_tag="ACHKW0_001372"
     CDS             complement(17..547)
                     /gene="ydeS"
                     /locus_tag="ACHKW0_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7474237.1"
                     /translation="MKYNNIIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPVVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDANMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            complement(561..3212)
                     /locus_tag="ACHKW0_001373"
     CDS             complement(561..3212)
                     /locus_tag="ACHKW0_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HFO7474238.1"
                     /translation="MTAFRAAFKAYRMHQVLILPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNADLIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPELWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYNSGSSNSSNSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIALGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGKDK
                     NSNCIVEYKLPEVSPGTLLNQQTAICR"
     gene            complement(3254..3964)
                     /locus_tag="ACHKW0_001374"
     CDS             complement(3254..3964)
                     /locus_tag="ACHKW0_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7474239.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDIPHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            complement(4127..4231)
                     /locus_tag="ACHKW0_001375"
                     /pseudo
     CDS             complement(4127..4231)
                     /locus_tag="ACHKW0_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4663..4871)
                     /locus_tag="ACHKW0_001376"
                     /pseudo
     CDS             complement(4663..4871)
                     /locus_tag="ACHKW0_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(5183..6505)
                     /gene="hipA"
                     /locus_tag="ACHKW0_001377"
     CDS             complement(5183..6505)
                     /gene="hipA"
                     /locus_tag="ACHKW0_001377"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HFO7474240.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGRVRALAVERFDRRWNTERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALRDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGSK"
     gene            complement(6505..6771)
                     /gene="hipB"
                     /locus_tag="ACHKW0_001378"
     CDS             complement(6505..6771)
                     /gene="hipB"
                     /locus_tag="ACHKW0_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HFO7474241.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(6980..12400)
                     /locus_tag="ACHKW0_001379"
     CDS             complement(6980..12400)
                     /locus_tag="ACHKW0_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECSE_1600 family autotransporter"
                     /protein_id="HFO7474242.1"
                     /translation="MNRIYRVIWNCTLQVFQACSELTRRAGKTSTVNLRKSSGLTTKF
                     SRLTLGVLLALSGSASGASLEVDNDQITNIDTDVAYDAYLVGWYGTGVLNILAGGNAS
                     LTTITTSVIGANEDSEGTVNVLGGTWRLYDSGNNARPLNVGQSGTGTLNIKQKGHVDG
                     GYLRLGSSTGGVGTVNVEGEDSVLTTELFEIGSYGTGSLNITDKGYVTSSIVAILGYQ
                     AGSNGQVVVEKGGEWLIKNNDSSIEFQIGNQGTGEATIREGGLITAENTIIGGNATGV
                     GTLNVQDQDSVITVRRLYNGYFGNGTLNISNNGLINNKEYSLVGVQDGSHGVVNVTDK
                     GHWNFLGTGEAFRYIYIGDAGDGELNVSREGKVDSGIITAGMKETGTGNITVKDKNSV
                     ITNLGTNLGYDGHGEMNISNEGLVVSNGGSSLGYGETGVGNVSITTGGMWEVNKNVYT
                     TIGVAGVGNLNISDGGKFVSQNITFLGDKASGIGTLNLMDATSSFDTVGINVGNFGSG
                     IVNVSNGATLNSTGYGFIGGNASGKGIVNISTDSLWNLKTSSTNAQLLQVGVLGKGEL
                     NITTGGIVKARDTQIALNDKSKGDVRVDGQNSLLETFNMYVGTSGTGTLTLTNSGTLN
                     VEGGEVYLGVFEPAVGTLNIGAAHGEAAADAGYITNATKVEFGSGEGVFVFNHTNNSD
                     AGYQVDMLITGDDKDGKVIHDAGHTVFNAGNTYSGKTLVNDGLLTIASHTADGVTGMG
                     SSEVTIASPGTLDILASTNSAGDYTLTNALKGDGLMRVQLSSYDKMFGFTHATGTEFA
                     GVAQLKDSTFTLERDNTAALTHAMLQSDSENTTSVKVGEQSIGGLAMNGGTLIFDTDI
                     PAATLAEGYISVDTLVVGAGDYTWKGRNYQVNGTGDVLIDVPKPWNDPMANNPLTTLN
                     LLEHDDSHVGVQLVKAQTVIGSGGSLTLRDLQGDEVEADKTLHIAQNGTVVAEGDYGF
                     RLTTAPGDGLYVNYGLKALNIHGGQKLTLAEHGGAYGATADMSAKIGGEGDLAINTVR
                     QVSLSNGQNDYQGATYVQMGTLRTDADGALGNTRELNISNAAIVDLNGSTQTVETFTG
                     QMGSTVLFKEGALTVNKGGISQGELTGGGNLNVTGGTLAIEGLNARYNALTSISPNAE
                     VSLDNTQGLGRGNIANDGLLTLKNVTGELRNSISGKGIVSATARTDVELDGDNSRFVG
                     QFNIDTGSALSVNEQKNLGDASVINNGLLTISTERSWAMTHSISGSGDVTKLGTGILT
                     LNNDSAAYQGTTDIVGGEIAFGSDSAINMASQHINIHNSGVMSGNVTTAGDVNVMPGG
                     TLRVAKTTIGGNLENGGTVQMNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPT
                     DKMNVKGDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVY
                     TLAKGKGNDEKNWYLTSKWDGVTPPDTPDPINNPPVVDPEGPSVYRPEAGSYISNIAA
                     ANSLFSHRLHDRLGEPQYIDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVL
                     QLGGDLAQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQ
                     NDANKTGAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEE
                     TLNTWYVQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDG
                     KQREFQPYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNV
                     GVQLGDKGYSDTQGMLGVKYSW"
     gene            complement(12931..14523)
                     /gene="lsrK"
                     /locus_tag="ACHKW0_001380"
     CDS             complement(12931..14523)
                     /gene="lsrK"
                     /locus_tag="ACHKW0_001380"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071518 - autoinducer-2 kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009372 - quorum sensing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HFO7474243.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPTSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(14602..15555)
                     /gene="lsrR"
                     /locus_tag="ACHKW0_001381"
     CDS             complement(14602..15555)
                     /gene="lsrR"
                     /locus_tag="ACHKW0_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HFO7474244.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            15804..17339
                     /gene="lsrA"
                     /locus_tag="ACHKW0_001382"
     CDS             15804..17339
                     /gene="lsrA"
                     /locus_tag="ACHKW0_001382"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HFO7474245.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQVLIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            17333..18361
                     /gene="lsrC"
                     /locus_tag="ACHKW0_001383"
     CDS             17333..18361
                     /gene="lsrC"
                     /locus_tag="ACHKW0_001383"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HFO7474246.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            18361..19353
                     /gene="lsrD"
                     /locus_tag="ACHKW0_001384"
     CDS             18361..19353
                     /gene="lsrD"
                     /locus_tag="ACHKW0_001384"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HFO7474247.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            19365..20387
                     /gene="lsrB"
                     /locus_tag="ACHKW0_001385"
     CDS             19365..20387
                     /gene="lsrB"
                     /locus_tag="ACHKW0_001385"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HFO7474248.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGMLVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            20414..21289
                     /gene="lsrF"
                     /locus_tag="ACHKW0_001386"
     CDS             20414..21289
                     /gene="lsrF"
                     /locus_tag="ACHKW0_001386"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HFO7474249.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFILKGCGALDWGMQSRLSRIF
                     NPKTGKTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            21313..21603
                     /gene="lsrG"
                     /locus_tag="ACHKW0_001387"
     CDS             21313..21603
                     /gene="lsrG"
                     /locus_tag="ACHKW0_001387"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HFO7474250.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            21660..22418
                     /gene="tam"
                     /locus_tag="ACHKW0_001388"
     CDS             21660..22418
                     /gene="tam"
                     /locus_tag="ACHKW0_001388"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HFO7474251.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIEYVADLGCGPGN
                     STALLNQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(22422..23336)
                     /gene="yneE"
                     /locus_tag="ACHKW0_001389"
     CDS             complement(22422..23336)
                     /gene="yneE"
                     /locus_tag="ACHKW0_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HFO7474252.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLHFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(23543..24994)
                     /gene="uxaB"
                     /locus_tag="ACHKW0_001390"
     CDS             complement(23543..24994)
                     /gene="uxaB"
                     /locus_tag="ACHKW0_001390"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HFO7474253.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(25221..26639)
                     /locus_tag="ACHKW0_001391"
     CDS             complement(25221..26639)
                     /locus_tag="ACHKW0_001391"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7474254.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(26778..27137)
                     /gene="yneG"
                     /locus_tag="ACHKW0_001392"
     CDS             complement(26778..27137)
                     /gene="yneG"
                     /locus_tag="ACHKW0_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HFO7474255.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(27137..28063)
                     /gene="glsB"
                     /locus_tag="ACHKW0_001393"
     CDS             complement(27137..28063)
                     /gene="glsB"
                     /locus_tag="ACHKW0_001393"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HFO7474256.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(28127..29515)
                     /gene="sad"
                     /locus_tag="ACHKW0_001394"
     CDS             complement(28127..29515)
                     /gene="sad"
                     /locus_tag="ACHKW0_001394"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HFO7474257.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            29616..30497
                     /gene="yneJ"
                     /locus_tag="ACHKW0_001395"
     CDS             29616..30497
                     /gene="yneJ"
                     /locus_tag="ACHKW0_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7474258.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAIVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            30574..31688
                     /gene="yneK"
                     /locus_tag="ACHKW0_001396"
                     /pseudo
     CDS             30574..31688
                     /gene="yneK"
                     /locus_tag="ACHKW0_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
     gene            31838..33028
                     /gene="ydeA"
                     /locus_tag="ACHKW0_001397"
     CDS             31838..33028
                     /gene="ydeA"
                     /locus_tag="ACHKW0_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HFO7474259.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGSGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(33053..33718)
                     /gene="marC"
                     /locus_tag="ACHKW0_001398"
     CDS             complement(33053..33718)
                     /gene="marC"
                     /locus_tag="ACHKW0_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HFO7474260.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            33930..34364
                     /gene="marR"
                     /locus_tag="ACHKW0_001399"
     CDS             33930..34364
                     /gene="marR"
                     /locus_tag="ACHKW0_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HFO7474261.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKKLTADEVATLEHLLKKVLP"
     gene            34384..34767
                     /gene="marA"
                     /locus_tag="ACHKW0_001400"
     CDS             34384..34767
                     /gene="marA"
                     /locus_tag="ACHKW0_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HFO7474262.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            34799..35017
                     /gene="marB"
                     /locus_tag="ACHKW0_001401"
     CDS             34799..35017
                     /gene="marB"
                     /locus_tag="ACHKW0_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HFO7474263.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(35074..36513)
                     /locus_tag="ACHKW0_001402"
     CDS             complement(35074..36513)
                     /locus_tag="ACHKW0_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HFO7474264.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHTRG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(36538..38211)
                     /locus_tag="ACHKW0_001403"
     CDS             complement(36538..38211)
                     /locus_tag="ACHKW0_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HFO7474265.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSYES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            complement(38267..38578)
                     /locus_tag="ACHKW0_001404"
     CDS             complement(38267..38578)
                     /locus_tag="ACHKW0_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HFO7474266.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            complement(38606..39928)
                     /locus_tag="ACHKW0_001405"
     CDS             complement(38606..39928)
                     /locus_tag="ACHKW0_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HFO7474267.1"
                     /translation="MGLMASFERGMERFLVPVAIKLNSQKHVAAVRDGFVFTFPIIMA
                     SSLIILINFAILSPDGFIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVA
                     RNMAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALIT
                     SEIFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLTLISPAGLN
                     DLIYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENL
                     SWAAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSF
                     IPGIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTT
                     PGPLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            complement(40043..40354)
                     /locus_tag="ACHKW0_001406"
     CDS             complement(40043..40354)
                     /locus_tag="ACHKW0_001406"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7474268.1"
                     /translation="MKKILLVCAAGMSTSMLVKRMIDHANAISLEVNISALAIAEAKG
                     KIKNNEVDVVLLGPQVRFQKPEIEAVAQGKMPVAVIEMKDYGTMNGQAVLEFAMKLLQ
                     E"
     gene            40553..41251
                     /locus_tag="ACHKW0_001407"
     CDS             40553..41251
                     /locus_tag="ACHKW0_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7474269.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(41296..42195)
                     /gene="eamA"
                     /locus_tag="ACHKW0_001408"
     CDS             complement(41296..42195)
                     /gene="eamA"
                     /locus_tag="ACHKW0_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HFO7474270.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGACTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            42390..43577
                     /gene="ydeE"
                     /locus_tag="ACHKW0_001409"
     CDS             42390..43577
                     /gene="ydeE"
                     /locus_tag="ACHKW0_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HFO7474271.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            43704..43799
                     /gene="mgtS"
                     /locus_tag="ACHKW0_001410"
     CDS             43704..43799
                     /gene="mgtS"
                     /locus_tag="ACHKW0_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HFO7474272.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            43799..43903
                     /gene="mgtT"
                     /locus_tag="ACHKW0_001411"
     CDS             43799..43903
                     /gene="mgtT"
                     /locus_tag="ACHKW0_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtT"
                     /protein_id="HFO7474273.1"
                     /translation="MNGDNPSPNRPLVTVVYKGPDFYDGEKKPPVNRR"
     gene            complement(44018..44908)
                     /gene="dgcZ"
                     /locus_tag="ACHKW0_001412"
     CDS             complement(44018..44908)
                     /gene="dgcZ"
                     /locus_tag="ACHKW0_001412"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HFO7474274.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTSALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNAEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
BASE COUNT        11735 a        11234 c        10255 g        11921 t
ORIGIN      
        1 tcatatctga ctctgattat tgaaattcaa gggtaaaggt tgccgaagcc ctcaccagtc
       61 ccggattgac ggacttctga gttgacttca gacgagcgga gtaaggcaaa atattgttct
      121 gttctggtac cagtgggatc cactgcatcc cggcatgaag gtcattcagt ttcaccggac
      181 gcatatttgc gtccaatatt tctataccca aaccggaggc cgtattgctt ccttcatcca
      241 gtttcaacaa agtattattt tctgcatcct caataccgtt aaatgcaacc cgaacccccg
      301 ttgtcccttt gctgcattca cttaacgtaa tctgaaaagg gacgactgga cttgtgctac
      361 cggtcgttgg aaattgtctg gcactgtttt tttggagatc tacggtaaaa ttaagcgaat
      421 ccgatgagac tgtgcagcca taatcgagga cgcgcccgct aattttaata acgctatctg
      481 cggataaagc agaataggtg gttaacccca gacataaacc gaggaaaata atgttattgt
      541 atttcataat ctattgttcc ttagcgacag attgctgtct gctggttcag taaggtacca
      601 ggagaaactt caggaagctt gtactcgaca atacagtttg agtttttatc tttgccccat
      661 gaaacctgta atttccctga ctgtggaagt ccagtcagat aaacctgacc gttttctgcg
      721 acaatgctgc catttttatt ctctccgtgt gtgacaattg caccgaatgg aacgctctta
      781 ttaccgtact tcaacgtcat taatactttc ccgccgattt gtgcattaaa tgttgctctg
      841 gcaatagcac cgtgagttgg gatgacagtg accacggttt catccagttc aacattatct
      901 gcaagggaat tcgcgttaag agcaacacgg ttttctctat attctgtcgc aaatggtaat
      961 atggcatagc cacgccagtc ggtatgaatt ccggtctggt tctctatttt gacattatca
     1021 gcgccaggag ccttaaccag aaccattgtg tcgcccagcg gctgtccaaa ggtgatgcca
     1081 tcagcatgag caataatccc accactcatt ccgtaataaa tctggctgct gtcaccattc
     1141 cgactgtaac cgacattcgt attaccgtaa gctccacgat aattaagaga actgtaacca
     1201 ctggtgccag acgatgtatt acctccgtgg gtgttaccga cctgaacgct ataattcagg
     1261 ttattatccg gcagcagagt gccataaacc cccgatagat tggtcatgcc gcctttcaaa
     1321 tcgtttgaca tactgtaact ggcgtttgaa ttacgaaatg ccgactgact gtctgtacgc
     1381 atccaatgac tgaagggaac attaagcgta aaagcgagta aatgatcccg atcgttttgc
     1441 catatattat tggaatagct gtaattcagc gaagtcgtaa tatcaccaaa cggcacattt
     1501 aatccaaatg atatttgctg gtcgctgcgt gacgtgttcc agtaactttg gcgactggca
     1561 ctgaaaaatg tcgtaccgta atttccaagc tgctgagaga tgcttatttg ttcctgacca
     1621 cgcttactgt agaacagatt aaaataatca ataaattgtg tctgctcatt ggtgtctccg
     1681 gtaggaggct tgacggtgta accactcatt cgactgtagg cactgtcgga taagttataa
     1741 aaaccttgcg tagaatagcg atatcctgcg acctggatat tggttcctgt ctggtagaag
     1801 gatttgctgt agacggattt aaccgattgc ccgctgtgac gggtgccatc tgcaagtgtc
     1861 gtattggcct gcgtgatatc gaaagaaaag gcaccaaaaa gtcctaaatc tttaccaata
     1921 ccaaggttga aggcctgata atcttctgca atttgcattc cgccataagg tgtccagttt
     1981 ccttccagtc catgcatcaa gctaccttgt atgaacttgg gggagctttg caggttattt
     2041 ccactacgat attcccccat ggcaagcgca taacgcgtat atccagcacg ttggagaacc
     2101 ggaacagacg aataaggaac atataatgtc tgaatactgc cgtctgcctc ttttatggtt
     2161 acttgcagat caccaccatt ggcggcagag ttgatatcat caatagtaaa agggccgggt
     2221 ggaacagtag tctgataaac atcgtatccg ttctgtttta cactcacttg tgcggtgcca
     2281 agggcaatac catgaatcac cggagcgaaa ccatgttggc tatcgggcaa catcgcttct
     2341 gttgaattta ttttgaggcc acgaaagttc acactatcaa aaatatcgcc atcggtataa
     2401 ctatcaccta ccgttaaccg tgagcgtaag ggaataatgt cacgttcagc cgacgtattg
     2461 atatgctgcc atttattgct gttagatgaa ttgctactcc cactgttata actccacgtt
     2521 gagttatcgc gcagtcgcca actaccaatg ttgatgccac tctgtaaatt caaatatgca
     2581 tagttggatt ttcctgcatt atggttacta cgattattaa tggaattacc attaaaacta
     2641 taatttagaa gcccagcatt tattccttct tcccacagtt caggagaaac atagccgcga
     2701 gccattctgc caacataaat ttgcggaacg gagagtgaca aatgttgctg accaacatca
     2761 aactcagttg atgcgtcggc caaacgatcc tgaagtggtg tgcatgcgct gttgtccggc
     2821 acatgttttt ctgcagaatg ttccttattg tctaataacg cagatttttt tatgccaaga
     2881 ctgacaagaa ggtctgtact cagacatggg atgagatctg cgttattatc atctgcaata
     2941 aaagtaatat cccgactagt cataaattcg tcgttcaaat aaatgtcgac ccgatatgta
     3001 ccaggtggtg cctcgcgtcc cttagtaaag gcggataaat cgacagattc agcaagatca
     3061 ttgcttaaaa accgtggatt aaaataatac tctgcatcaa cgggaaaaac cgcagttgct
     3121 aagccaaggg caatggtaag ccgtgcaaag cgtggtagta ttaacacctg atgcatacga
     3181 tatgccttaa atgcggcacg aaaggctgtc attattaatt ccgattgagt ttattattaa
     3241 ttaagtcact ggcttactgc atggttgcct taatacgcgg agttaatgca ccgtaatcat
     3301 tgatcgtctg aaaactgata tccccggttg cagcatgcgg gatatccacc gatacttcac
     3361 cttttggcgg caccatggtg tttgggagat tgctatttcc tgctttcata tttgttacgg
     3421 taatgaagta gggcgttgga ttaataagag tgagtttgct accgctgcga cgaaaccgaa
     3481 gcattgctgg cgcttcttca ggtgccattg ccaggttcgt ggggcgataa aacattttta
     3541 tgcggctaat aattgccagc tgtaatgtat tctcattctt ctggtctttt tccatagcag
     3601 gaatagcttt aacattcacc cagaacaggc tttcacggtc gcctggtaac tgatgattag
     3661 tagcgttgat tatccgcagc gtattctctt ttttaccttg catggaaaaa agcggtgggg
     3721 taatgacaaa ctgggtgtct ttttggtcac ctgcattttc tatccatgac tgaatgagat
     3781 agacactggc aggatcatta ttcgtgacag gcaataaaac ctgtttctgg tttgccggat
     3841 aaattacccg cgtcgcaccc agtgcgacag ctgcgttggc aacggtgcta tggcaagcaa
     3901 agagcaataa aagtaacact gtcgccataa aggaaataga gtagggggta cgcgtagttt
     3961 gcatctgatg cattctccat gtgttaagag gataagcggg aaaaccgtta catcagacaa
     4021 tcgattaatg ttgctaatac attggcgagt cgccgggttt ccagacttaa tgcgtttgta
     4081 ataaatgttg gttctctttt aataggaaaa ccttgctaca aagtaaatta acagtggtcg
     4141 ttactgattc atcaccctcg gctgcgctta ccgcagcaga cgacatcgcc aacacagaaa
     4201 cgacaatcat accaacatgt ttgagtttca taattctcca aaatttatat ttaagtagct
     4261 gaataaagag ggttaacaat taagtcattc tgttattgct taaaaacaag aatcattctt
     4321 gattggttgt tgccctgaga atatagtttc cagggttatt acatttcacg aagaacgata
     4381 atatctttaa atcacaagga cacctaatca caacaagttt attgtgcact tgttgaagag
     4441 agtatttgca aaaacatttt atgtcgctaa ataacatgac gatttcatta tgcatgttat
     4501 ttataaaccg tgactaaaca gaatattatt gcgaacaagg gaagtgtcag gaaaggtcaa
     4561 gggctaccaa tggttaatat ggctataaat cattagctat ggttaatgtt tgcttgctga
     4621 aactaacacc gaattgctct aaacagataa gtgcagggtt tattaaagct gaccgacaga
     4681 ctttcttaac ctggtaatat ctctcttggg tgactctcca aacatccgcg aatattcccg
     4741 actgaaatgg gatagctttc gtaaccgaca gcataggctg ctgtggtgac atcgtaatgt
     4801 tcattcagca tcggtcgcct gacttcattg agacgtagcc atttctgata ccggagtgga
     4861 ctcattcccg cttgatccgt ccttaaataa gtaacagcac cctctggtgt tttcgtatgc
     4921 agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc aggctatttc
     4981 tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc tgaacgaata
     5041 acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat atacattatg
     5101 cgcaccagta taaaccaagg aagaatccag tcgttggcgg tcatgattgt cgtgctcatt
     5161 aacaacgacc aaaccccata tctcacttac taccgtattc tcggcttaac cgtccatgca
     5221 acctcaacac attggtttca actgccgtca ccacgttctc cggaaaatct gtcggtaatg
     5281 aagtcttcac gttatccagt gctgccggaa tcattctggc aaagtcactt aggatttcat
     5341 gcatctgcac ttccgggaat ctcagcacct ttgctgtcgc caaaaaatgt cgcggataaa
     5401 ttttatcgat tgccgttttt ttgcctttgg atgcgttaag ccccattgcc agtttgagat
     5461 cgctgatgtg tattcccgta ccgccaagga ccggaaatgc tgaaatgatg tcgtaaaatg
     5521 gcgtgagtcg ataactgccg ccagcctgaa taaatacgga gaagtttttt gcatggccat
     5581 ccgttgcgcc aatcaaccac tggaagacct ggaatttcat aaaatcataa cgatcgcgaa
     5641 gcgcctcgct ggaccccatc aaaaacgcca tgatccgcgc gatgcctggg cctccatctg
     5701 attcatattt cacagatgaa ggtaaaccga atgtctgaca catatcctcc tgtggcaagc
     5761 gaagtaaaac cgttcgctca gtattccaac gcctgtcaaa acgttcgacc gctaacgcgc
     5821 gcacccttcc cgctttaatg atttctgcgt ccggaacatt caacccaagt tctttcgcca
     5881 gcagcagaca gtaatactca ttatcaacgc tttggctgag atcgagcgtc gcattgggct
     5941 gcctgatttc gccaatcggt aatttaatga tgtgcgtcgt cggcgttatt cctttcggaa
     6001 tgcaccagtc attgcctatt ctaagcagcg ctgtcttctc ctgcgcgcca gcaaccgaga
     6061 tgcgaaagtc attttcttct ctaatcatgc ccagagggat atctgcttta taagccgtta
     6121 atacttcttc aagcctggct tcagtaagct tttcccatgc cattatcggg cgcgttatgg
     6181 tttcattttc gggtaataac gttacggcac caacgctgtc tcgccctatt tctgacaata
     6241 aatcaaacgg ttgtctggat ttagcatgat aacgtttaac gatccggtca cgtacaatcg
     6301 ggctatcggg taacaggtta tcgaagaagt taaatacggc atctgaggtg atattccccc
     6361 tctgcaatgg cagcgaaagt gacaacggtc tggcataacg gctggctaac cactccggtg
     6421 catacttaaa ggtgtgcgcg ccgttggcta acttcgttaa ctcgcctacc cgctggttgt
     6481 tcatccaagt gacaagttta ggcattacca ctccagatct tgctgttctg ttgattctgg
     6541 cgaggcattt ttcgtgtcgc atagtgtcat tgagagttca agcgactgta aaatcttaaa
     6601 aaatgtcgtg agcgtggtat tgtcagggtt gttttcgaaa ttggaaattg tcgcctgctt
     6661 aatgccaatt tttttcgcca gctcgctctg cgtccagcca ttttgctggc gaaccagttt
     6721 cattgcattc gccaattgcg ttgggctata gatcttctga aagctcatca tgtcatacgt
     6781 ccacagcaaa tttatccgct taaggggata ttatcagttt tatcctttag tgaggataaa
     6841 gtcaatctgg aaacacatct tatccgctgt acgggataaa cgctgcgata tccgcgatcg
     6901 cggataaaag ggaggcttta aagcctttga agtcggcaat attaagccgc atgccatctc
     6961 gacatgcggc ttatacggtt taccagctat atttcactcc cagcatgccc tgagtatcgc
     7021 tatagccttt atcacctagt tgcacaccga cattccccca caggctaagg ttgttattta
     7081 ctttcgcctc aaccccggta cgtacttcac cgagattacg cgcaccttca cggcctacgg
     7141 tttgaccatt catcttcacg gcgtagactt tgctgttgtt gatccaattc gcttcaatgt
     7201 aaggctggaa ctcacgctgt ttaccatcgt cacgctggtg atggctgttc aggtaggttt
     7261 tcaccccaag tcgcgtttgc acatttccgt cgccttccgt ttcaatgcgc gttccgtctt
     7321 tccgggtatg gtcggaatct ttcacaccca tccaggtgat ttgcgcctgt ggctggacgt
     7381 accaggtatt cagcgtctct tcgctgccgc taaatgttcc cgcttcaaag gtatacccac
     7441 cctcaacaga ggccgtcaca ccgcgagaat catagtcgtc agcagaacgg ttatcggaac
     7501 tgacgctgtt atcaaaccag ttatacagcg cccagctgtc aacataagcg ccggtcttat
     7561 tcgcatcgtt ctgataccag gtcgcgtaca gcccagcgct gtaaccgctg atgcgcccat
     7621 ccgatttata acccacacga ttactctgag tattactgtg ctgattagcg taacctgcca
     7681 tcacgccaag atgccagcga tcctgcgcgt tgctactcca ctgcgccaaa tcgccgccta
     7741 gctgcaatac atagcggtta gcctgagtat ttagctgacc gtcaccggcc cttgaacgtt
     7801 cgtgccctcc gacatgacgc atccacatac tgcttgccga cccctgagaa tgcagtgaat
     7861 ctatatactg cggctcaccc agacggtcgt gtaaacgatg gctaaacagc gagttggctg
     7921 cggcaatgtt gctgatatag cttccggcct ccgggcgata aactgatggg ccttccggat
     7981 ccacaacagg gggattattg atggggtcgg gcgtatccgg tggcgttacg ccgtcccatt
     8041 tactggtcag ataccagttt ttctcgtcat tccccttccc tttagccagc gtgtagacgt
     8101 aagccccagc ttcgacagtt ccggtggtca gcgcgaagtt acctgcagaa ttaccgccaa
     8161 cctcaatgag ttcaataccg ttgaccgttt gtgcaccgac gccgccaatg ttatcaaccc
     8221 gaacgcgagt gttcccttgg gtatcgcctt tcacgttcat tttatcggtg ggcgaattat
     8281 cgccgcccag cgtcgcgttg aacgtcatca ggccattgtt tccggtatag ttgccgttaa
     8341 cggtcagtac attccccggt ttcccccctt cgctattcat ttgaaccgtg ccgccattct
     8401 ccaggttgcc gccgattgtg gttttagcga cacgcagtgt ccccccaggc ataacgttca
     8461 catcacctgc agtggtgaca tttcccgaca tcacaccgct gttatggata ttaatgtgtt
     8521 gacttgccat attaatggca gagtcggaac cgaaagcaat ttcccccccc acgatatccg
     8581 tcgtaccctg atacgccgcg gaatcgttgt taagagtcag gatcccggta cccagttttg
     8641 tcacatcacc gctaccgctg atactgtgcg tcatcgccca gctacgctca gtggagatgg
     8701 tgagcaggcc attattgata acggaagcat cacccaggtt tttctgctcg ttgacgctga
     8761 gcgcgctgcc tgtatcaatg ttgaattgcc ccacaaagcg gctattatcg ccatccagct
     8821 ctacatctgt cctggcggtt gcgctcacga tacccttccc gcttatgcta ttacgcagtt
     8881 cgccagtcac gttttttagc gttaacagac cgtcattggc aatatttcct ctgcctaacc
     8941 cctgagtatt atcgaggctg acttccgcat ttgggctaat gctggttaac gcattgtagc
     9001 gtgcattaag cccctcgata gccagcgttc cccctgtaac attcaggttt ccgccacctg
     9061 tcagttcacc ctgactgatc ccacctttat ttaccgtcag cgccccctct ttgaacaaaa
     9121 cagtcgaacc catctgcccg gtgaatgtct ctaccgtctg cgtcgatcca ttaagatcga
     9181 cgatggccgc gttgctgatg ttcagttccc gggtgttgcc aagtgcacca tccgcatcgg
     9241 tacgtaatgt ccccatctga acgtaggttg ccccctgata gtcgttctga ccgttggaaa
     9301 gcgaaacctg tcgcaccgta ttgattgcca gatccccttc accaccgatt tttgccgaca
     9361 tatcggccgt tgcgccatag gctccgccat gttcggctaa cgtcagcttt tgcccaccat
     9421 ggatgttcag cgctttcagc ccatagttaa cgtacaaacc atcacctggt gcggtcgtga
     9481 ggcggaatcc ataatcaccc tcggcgacca ccgttccgtt ttgcgcaatg tgtaacgttt
     9541 tgtccgcttc cacctcgtcg ccctgtaaat cacgtaacgt taatgagcca cccgacccaa
     9601 taaccgtttg cgccttcacc agttgaacgc cgacatggct atcgtcgtgt tccagcaaat
     9661 tgagcgtcgt cagagggtta ttcgccatgg gatcattcca cggtttaggc acgtcgataa
     9721 gcacgtcgcc cgtcccgttt acctgatagt tacggccttt ccaggtgtag tcacccgcgc
     9781 cgacaaccag cgtatcgacg ctgatatatc cctctgcaag cgtcgcagca ggaatatccg
     9841 tatcgaaaat gagcgtaccg ccattcatgg ccagtccacc aatggattgc tctcccactt
     9901 ttaccgatgt ggtattttca ctgtcagact gcaacatcgc gtgagtaagc gcagcggtgt
     9961 tgtcgcgttc cagagtgaag gtactgtctt tcagttgggc aacaccggcg aattcagtcc
    10021 ctgttgcatg ggtaaagcca aacatcttgt cgtaggatga cagctgcact cgcatcaagc
    10081 catcgccttt gagcgcattg gtcagcgtgt aatctcctgc actgttcgtt gatgcgagaa
    10141 tgtcgagcgt accggggctt gcaatggtga cttcactcga acccatgccc gttaccccat
    10201 ctgccgtatg agacgcaatg gtcaggaggc cgtcattgac cagcgtttta ccgctataag
    10261 tattccctgc attgaacacc gtatggcctg catcatggat cacttttccg tctttatcgt
    10321 cacctgtaat cagcatatcg acctggtagc cggcatcact gttattagta tgattaaaga
    10381 caaaaacacc ttcgccagaa ccaaactcta ctttcgtcgc gttggttata tacccggcat
    10441 ctgccgccgc ctcaccgtga gctgcaccaa tgtttagcgt tcctacagca ggttcaaaaa
    10501 cacctaagta aacttctcca ccttcgacat tcagcgtgcc gctattcgtc agggttaacg
    10561 tacccgtacc agatgtccct acgtacatat tgaatgtttc aagaagagag ttctgcccat
    10621 ccaccctcac gtcgccctta cttttgtcat tgagagctat ctgtgtatca cgcgctttaa
    10681 ctatacctcc ggtggtaata ttcagttcac ccttgcctaa tacaccgacc tgtagcaatt
    10741 gtgcgttagt agatgatgtc tttaaattcc agagactgtc cgttgaaata ttaactatcc
    10801 ccttaccgga ggcatttcct ccgataaatc catagcccgt tgaattaagg gtggcaccat
    10861 tactgacatt tacgataccg ctaccaaaat taccgacatt gatacccaca gtatcgaacg
    10921 atgatgtcgc atccatcagg ttcagtgtgc cgataccgct tgctttatcg cccaaaaaag
    10981 taatattttg cgatacgaac ttaccgccat cgctaatatt gaggtttccg acgcccgcaa
    11041 caccaatggt ggtatagaca ttcttattga cctcccacat cccccccgtg gtgatgctga
    11101 cattcccgac gccggtttct ccataaccga gtgagcttcc tccgttgctg acaacaagcc
    11161 cttcattact gatattcatt tcgccgtggc cgtcataacc aagattagtt ccgagattag
    11221 tgataacgga gttcttatcc ttaacagtaa tgttgcctgt gcctgtttct ttcatccccg
    11281 cagtgataat tcccgaatct actttacctt cacgcgagac attaagttca ccgtcgccag
    11341 catcaccgat atagatatag cggaaagctt cgcccgttcc gaggaaattc caatgccctt
    11401 tatcggtcac gttgacgaca ccgtgggaac cgtcctgaac gcccactaat gaatattctt
    11461 tgttattaat tagtccatta ttggaaatat tgagtgtgcc attaccgaaa tatccattat
    11521 agagtctgcg taccgtgatg acagagtctt gatcctgcac attcagggtt ccgacaccgg
    11581 tggcattgcc accgataatc gtattttcag ccgtaatcag tccaccctcg cgaatagtcg
    11641 cctccccagt tccttgatta ccaatttgaa attcaattga ggaatcgtta ttttttatta
    11701 gccactcgcc acccttttca acgacaacct ggccattact gcccgcctga tagcctaaaa
    11761 tggcgacgat tgaactcgtg acgtaaccct tatccgtaat atttaatgag cccgtaccgt
    11821 aactccctat ttcgaataat tcggtcgtca gaacagagtc ctctccctca acattgaccg
    11881 tcccgacgcc tcctgtcgaa gaacctaatc ttaaatagcc tccatcgacg tgacccttct
    11941 gtttaatatt cagcgtcccc gttccggatt gacccacatt taaaggcctt gcattatttc
    12001 cgctatcata caatcgccag gtgccaccca aaacattaac ggtgccctct gagtcctcat
    12061 tagcgccaat gacgctggta gtaatagtgg ttaaggaggc attaccgcca gccaaaatat
    12121 taagcactcc agtgccatac cagccaacca ggtaggcatc ataagcaaca tcagtatcaa
    12181 tattggtaat ctgatcatta tcaacttcca gacttgcacc agacgctgaa ccgcttagtg
    12241 ccagcaaaac acccagcgtc aatctactga atttcgttgt cagtccagag gatttacgca
    12301 aattaaccgt cgatgtttta cctgccctgc gagttaattc cgagcaggcc tgaaatacct
    12361 gtagagtgca attccatatc acgcgataga ttctattcat ataaacgctc catatacaaa
    12421 caatacagcc acgaaagaga tgagtcgtca gacattaaaa atgaaactta ttaaattgtc
    12481 agaggtctgt attaagtgtt agttggaggt gggaaggtgt caatttggct aatttgctaa
    12541 tactttcgaa ttgatagatt aaaaaatgca tagttcttat tttatttaaa aatgaactat
    12601 gagttattgt tgattaataa gaaaactgtt tctttcaata ggaaaattat catcatcata
    12661 tttaacaaag aatagcacta attgctaaaa atcgaagttt attaaacccc ttttgcatcc
    12721 attaatgtct ttaggaatat tcgctataaa ataagggtta tgcttaatgc tttaagaaaa
    12781 atagcaattt tcccttgaat atagtactgg tgatggaacg atgaatctgc aagctggcat
    12841 ttaacaagcc agctctaaaa gaagggaaat aagaaaaact atactcaaaa actaacagcc
    12901 acggtcatca tgatgtggct gtcaatgaaa ctataaccca ggcgctttcc ataacgacgt
    12961 cgtcagtcca tgatcaacca gccccagctg atcctgataa actgcctgcc acttatcgcg
    13021 tgaatcctga taaagttcat gcttttccgg gtctggtgtg tgcgtccgtt cccagcgaac
    13081 caggcgttct ccggtttctg ccattgatga aaaaattccg gcaccgacgc cagctgcaat
    13141 ggcacatcct aatgcggtgg cttctttgac taccggaata ttgacgggta atcccgagac
    13201 atcagcgaga atttgactcc ataatttccc ttttgaacct ccgccagcaa aaaccagcga
    13261 ggtgggatga atattcgaga aatcagcaat ttgctgcaag ttacacgctg atacaatcgc
    13321 cgcattttct tccagcgcac ggaacaatgt cgctttgtta catttatccg ggtcaatgga
    13381 caagttaata aaggaaggcg cagcgtgata ccaggtttta aagcgcattc tgtcggagaa
    13441 gatcggcatt acgccccacg acccaggcgg cacccgactg gccatctctt ccagcagcgt
    13501 ataggtgtcg atgcctaaac gttcggcaat cagtttttct tcggcacaga aagcatcgcg
    13561 gaaccagcgc atggtgagtc cggtaaaaaa gcttatagat tcagcttgta ccatgccagg
    13621 aataacatga ggattaacgc gcacgttcat ttctgggtct gtcaccggcg cggctaaatt
    13681 tacaacttgc tgccagaatg tgccgccaag aaccgcggtt tgtgccggac gcacaacgcc
    13741 taacccaagg caaccaagct gcacgtcgcc tcctccaacg accaccggag tgcccgcctt
    13801 cagaccgcag agttccgccg cttgtgaact taccacgccc agcaatgtgc cggtttcttt
    13861 gacaggagaa agaatatcgg cacgcaggcc agccatatcc agcaatgcag gtttccagtc
    13921 acgggtggtt agatcaagaa gtcccgtggt gccagcgtta gagggatcca ccgccagttc
    13981 gccgctgagc atataggcca gccagtcgct gatcatggtg atggttgatg cctgacggta
    14041 aatatcggaa cgatggtgcg ccagccaaag taatctgggg atggcactta aagccagtgt
    14101 ttgtccggtc gcgcgataaa cttcgttttc aaaggtattg ttgtgcagtt ctttaagttc
    14161 gctaacttcg cgtgccgctc tggcatccac attggcgcag gcccagatcg gggctccttc
    14221 attattatat aaaacaatgc cttcacgcat cgaacatgcc gaaacggcag cgatatactc
    14281 cggggctatg ccggcgttgt gcagcgcctg gcgcatacac tcacacgcca gttgccagtt
    14341 tttgttgaga tcaaattcca tagaaccagg aacgtccggt actgccaggt gccgccactc
    14401 cgcctgtccc actgctattt gattgccttc caggtcgaat atcacagccc gaatacttcc
    14461 ggtacctgca tccagcgcca tcaggtaata ctttgattct gaaggggtaa agagtcgagc
    14521 cattagatta tcctcggctt atgcgttctg tctgttcctc tatacgttct ccatcattcc
    14581 cggtaataag gtcatgcaaa tttaactacg taaaatcgcc gctgctgtgt cctgatcggt
    14641 aaccagtgcg ttgatataac cgcctttcat tgcagcggca attgcttcgg ctttattttc
    14701 tccccctgcc acgccaaccc ggacgggtat ggtcttcagc gagcttaaag gtaagccaat
    14761 cagttcgtta tgtattttaa tatccgtgac aacgtcacct tttgcatcaa aaaagtagcc
    14821 taaaatgtcg ccaaccgccc cttttcgacc aatcattaac tgttcgccct ggctgatata
    14881 accggagcga atgatcgtcg cgtcgtcctg ttgactgacg gccccaatgc cgacaatcgc
    14941 cacatcggct gcttgcgcgg ctaacagaac atctttgacg caattttcat tttttagcgt
    15001 acgggcaatg tcagcggagg atgcccgcaa cggagccgga ataatattca cactgcacgc
    15061 cgcgttaagc tgcccgatac ccgtcatata agaaccgacg ccaccggaga gcgtgaccag
    15121 gcgaatttgc tgtgacgaaa taaaaccact taagcgttgc agcgtattca tggttgcctc
    15181 gccaaaacca atcgccagca tctgttgtgg ttgaagtaaa ctcatcaaca tatgcgccgc
    15241 gcctatcccc agtcgcccgc cgacatcagc atccgcaagc ccagggatca cccggacatg
    15301 ttgcagcgaa aactgacgac gtaattgagt ttcatattcc agacagcctt caaagcgaga
    15361 attaatctgt acgcgaataa tgccggactg atgccctttc tccagcaagc gcgacacttt
    15421 caaacgggtc aggccgagac gatcgctgat ctcgctctgg gtcagcccgt cgtgatagta
    15481 aaaccacgcg atccgcgcga cctgttcttc ttcacacatt ccctgttctg aaattgccga
    15541 atcgttgatt gtcataattc attcttcact ttgaacatat ttaaatcttt aatgcaattg
    15601 ttcagttctt gcgcatttat atctgtgatg gcagccacag tttgactcta cgagcatgaa
    15661 caaacgcaac cgtgaaaatc aaaatagcat aaattgtgat ctattcgtcg gaaatatgtg
    15721 caatgtccac ctaaggttat gaacaaatta aaagcagaaa tacatttgtt caaaactcac
    15781 ctgcaaaact gaacggggga aatatgcaaa cgagtgatac ccgcgcgtta ccgctacttt
    15841 gcgcccgctc ggtttataaa cagtattcag gggtcaatgt cctgaaaggc atcgatttta
    15901 cgttgcatca gggggaggtc cacgccctgc tcggcggcaa tggtgccggt aaatcgacgt
    15961 taatgaagat tattgccggt attacccctg ctgatagcgg tacgctggag attgggggca
    16021 acaactacgc cagattaacg ccagttcatg ctcatcagct gggcatttat ctcgttcccc
    16081 aggaaccgct gcttttccca agcctgtcga taaaagaaaa catcctgttt gggatggcaa
    16141 aaaaacagct ctccatgcaa aaaatgaaga acttgctggc ggcgctgggc tgccagtttg
    16201 atctgcatag tctggcagga tcgctggatg tcgccgatcg ccaaatggtg gaaatcctcc
    16261 gcgggctaat gcgcgactcg cggattctga tcctcgatga acctaccgcc tcgcttaccc
    16321 ctgcggaaac cgaacgcttg tttactcgct tgcaagagct gcttgctact ggcgtgggta
    16381 ttgtttttat ctcgcataag ctgccggaaa ttcgccagat tgccgatcga attagcgtga
    16441 tgcgcgacgg aaccatcgcc ttaagcggca aaaccagcga actgtctacc gacgacatta
    16501 ttcaggccat caccccagcg gtacgggaaa aatcgctctc tgccagccaa aaattatggc
    16561 tggagttacc tggtaaccgc ccacaacatg ctgccggaac gccggtgctg acactggaaa
    16621 acctgaccgg cgaaggtttc aggaatgtca gcctgacgct caatgccgga gaaattctgg
    16681 gcctggctgg gctggtgggg gccggacgca cagaactggc cgagacgctc tatggtctgc
    16741 gtactttgcg tggcggacgc attatgctga atggtaaaga gatcaataaa ttatccactg
    16801 gagaacgttt actgcgcggt ctggtttatc tgccggaaga tcgccagtca tccggactga
    16861 atctcgatgc ttcgctggcc tggaacgtct gcgcccttac tcataacctt cgtggattct
    16921 gggcgaaaac cgcgaaagat aatgccaccc tggaacgtta tcgtcgggcg ctgaatatta
    16981 aatttaacca accggaacaa gctgcacgga cattatccgg tggcaaccag caaaaaatcc
    17041 tcattgccaa atgcctggaa gcttcgccgc aagtattgat tgtcgatgag ccgacgcgcg
    17101 gcgtggatgt ctcggcccgt aatgatatct accagctgtt gcgcagcatc gccgcacaaa
    17161 atgtggcagt gctgcttatc tcctccgatc tggaagagat cgaactgatg gcagatcgcg
    17221 tgtatgtgat gcatcagggc gaaattgccc actctgcact gaccgggcgc gatattaatg
    17281 tcgagactat tatgcgcgtt gccttcggcg atagtcagcg tcaggaggtg tcatgctgaa
    17341 gtttattcag aacaaccgtg aaatcacggc actgctggcg gtggtgctgc tgtttgcatt
    17401 acccggtttt ctcgaccgcc agtatttaag tgtgcaaacg ctgaccatgg tttatagcag
    17461 cgcgcaaatc ctgatcctgc tggcaatggg cgcgacgctg gtaatgctta cgcgcaatat
    17521 tgatgtttca gtgggttcga ttaccggaat gtgcgcagtg ctgttgggga tgttactgaa
    17581 cgcggggtat tcactacctg ttgcttgtgt cgcgacttta ctgcttggtt tgctcgcggg
    17641 atttttcaac ggtgtcctgg tcgcgtggct aaaaatccct gccattgttg ccacccttgg
    17701 cacgttaggg ttgtacagag gcatcatgtt gctgtggact ggcggtaaat ggattgaagg
    17761 gttacccgcc gaactgaaac agctctccgc accgctgctg tttggcgttt ccgcgattgg
    17821 ttggttgacg ataattctgg tggcatttat ggcctggctg ctggcaaaga cggcgtttgg
    17881 acgcagtttt tatgccacgg gcgataattt acagggcgct cgtcaactgg gtgttcgtac
    17941 ggaagccatt cgcattgtgg cattttcgtt gaacggctgc atggcggcac tggcgggaat
    18001 tgtgtttgct tcgcagattg gttttatccc caaccagacc ggtaccgggc tggagatgaa
    18061 agcaattgca gcctgcgtgc tgggcggcat tagtttgctc ggcggttccg gtgcgatcat
    18121 cggtgcggta ctcggcgcat ggttcctgac gcagatcgat agcgtactgg tgctgttgcg
    18181 cattccggca tggtggaacg attttatcgc gggcctcgtg ctgctggcgg tgctggtgtt
    18241 tgatggacgc ctgcgttgtg cgctggaacg taatctacgg cggcaaaaat atgcccgctt
    18301 tatgacgcca ccgccatccg ttaaacccgc ttcgtcaggt aaaaaacggg aggccgcata
    18361 atgcgtattc gctacggttg ggaactggct cttgccgcac tgctcgttat tgagattgtc
    18421 gcatttggtg caattaaccc gcgaatgtta gatctcaata tgttgctgtt cagcaccagt
    18481 gactttatct gcattggcat tgtcgcccta ccgctgacga tggtgattgt cagtggcggg
    18541 atcgatattt cgtttggttc gaccatcggc ctctgcgcca ttgcattggg cgtactgttt
    18601 caaagtggtg tgccgatgcc gctggcgatt ctcctgacct tactgctcgg cgcattgtgc
    18661 gggctgatca acgccggatt aattatctat accaaagtta acccgctggt gattacgctt
    18721 ggcacgctgt atctgtttgc cggaagcgcc ctactgcttt ccgggatggc cggagcgacg
    18781 gggtacgaag gtattggtgg attcccgatg gcgtttacag atttcgctaa cctggatgtg
    18841 ctgggactcc ccgttccgct gattatcttc ctgatatgtc tcctcgtttt ctggctctgg
    18901 ctgcataaaa cccatgccgg acgtaatgtg tttttgattg ggcaaagccc gcgcgtggcg
    18961 ctttatagcg cgattccagt taaccgcacc ttatgtgcgc tctatgccat gacggggctg
    19021 gcgtctgcgg tcgccgctgt gctgctggtg tcgtattttg gttcagcacg ttccgatctc
    19081 ggtgcgtcgt ttctgatgcc cgccatcacc gccgtggtgc ttggcggtgc caatatttat
    19141 ggtggttccg gttccattat cggcaccgcc attgcggttt tattagtggg atatttgcaa
    19201 caaggtttgc aaatggcagg agtgccaaat caggtgtcca gcgccctttc cggtgcgcta
    19261 cttatcgtcg ttgtcgtagg tcgttccgtt agcctgcatc gccagcaaat taaagagtgg
    19321 ctggcgcgtc gggccaataa cccattgcca taaaggatat cttcatgaca cttcatcgct
    19381 ttaagaaaat cgccttactt agcgctcttg gcattgccgc aatctctatg aatgtgcagg
    19441 ccgcagagcg tattgcattt attcccaaac tggttggcgt gggatttttt accagcggtg
    19501 gcaacggcgc acaacaagcg ggtaaagagc tgagcgttga tgtgacctac gacgggccga
    19561 cagaacccag tgtttctggt caggtacagt tgattaataa cttcgtcaat caaggttata
    19621 acgccattat cgtttctgcg gtttcgcctg atggcttgtg tccggcactg aaacgcgcca
    19681 tgcaacgtgg tgtgagagtg ctgacctggg actctgatac taaaccggag tgccgctctt
    19741 actacattaa tcagggaacg cccgcccagt tgggaggtat gttggtggat atggcggcgc
    19801 gtcaggtgaa taaagacaaa gccaaagtcg cgtttttcta ctcaagcccc accgttacgg
    19861 accaaaacca gtgggtgaaa gaagcgaaag cgaaaatcgc caaagagcat cctggctggg
    19921 aaattgtcac tacgcagttt ggctataacg atgccactaa atcattacaa accgcagaag
    19981 gaatattaaa agcgtatagc gatctcgacg ccattatcgc ccccgacgcc aacgccctgc
    20041 ccgctgccgc acaagccgca gaaaacctga aaaatgacaa agttgcgatt gtcggattca
    20101 gtacgccaaa cgtgatgcgt ccatatgtgg aacgcggcac ggtgaaagaa tttggcctgt
    20161 gggatgtggt tcagcaaggc aaaatttcag tgtatgtcgc ggatgcatta ttgaaaaaag
    20221 gatcaatgaa aacgggcgac aagctggata tccagggcgt aggtcaggtg gaagtctcgc
    20281 caaacagcgt tcagggctat gactacgaag cggatggtaa tggcatcgta ctgttaccgg
    20341 agcgcgtgat attcaacaaa gagaatatcg gcaaatacga tttctgatgt gcattactta
    20401 accggagtaa gttatggcag atttagacga tattaaagat ggtaaagatt ttcgtaccga
    20461 tcaaccgcaa caaaatatcc cttttatcct gaaaggttgc ggtgcgctgg attggggaat
    20521 gcagtcacgc ttatcgcgga tatttaatcc gaaaacgggt aaaaccgtga tgctggcctt
    20581 tgaccatggt tattttcagg ggccgactac cggacttgaa cgcattgata taaatatcgc
    20641 cccgctgttt gaacatgccg atgtattaat gtgtacgcgc ggcattttgc gcagcgtagt
    20701 tcccccagcg accaataagc cggtggtact gcgggcgtca ggtgcgaact ctattctggc
    20761 ggaattaagt aatgaagccg tggcgttatc gatggatgat gccgtgcgcc tgaacagttg
    20821 cgcggtggcg gcgcaggttt atatcggcag tgaatatgaa catcagtcga tcaaaaatat
    20881 tattcagctg gttgatgccg gaatgaaagt gggaatgccg accatggccg tgactggcgt
    20941 gggcaaagat atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa tcgccgctga
    21001 aatgggggcg caaattatca aaacctatta tgtcgaaaaa ggttttgaac ggattgttgc
    21061 cggatgtccg gtacccattg ttattgctgg cggtaaaaaa ttaccggagc gcgaggcgct
    21121 ggaaatgtgc tggcaggcta tcgatcaggg cgcttctggt gtggatatgg ggcgtaatat
    21181 tttccagtct gaccatccgg tggcgatgat gaaagccgta caggcggtgg ttcaccataa
    21241 cgaaacggct gatcgggcat atgaactcta tctgagtgaa aaacagtaac tgcggattta
    21301 aggagaagaa ttatgcacgt cacactggtt gaaattaacg ttcatgaaga caagattgac
    21361 gagtttatcg aagtttttcg ccagaaccac ctgggctctg tacaggaaga aggcaatttg
    21421 cgcttcgatg tcttacagga cccagaagtg aattcgcgct tttatatcta cgaagcctat
    21481 aaagatgaag acgcagtggc attccataaa accacgcccc actacaaaac ctgtgtcgcg
    21541 aaactggaat ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg gttgatgccg
    21601 tgaggcgaat tcatcagttt tatctacaat tggggtaacg cgctgacggg agtaaataaa
    21661 tgtctgactg gaacccctct ttatatctgc actttgccgc tgaacgatcg cgtccggcgg
    21721 tggagctgct tgccagagta ccgctggaga atatcgaata cgttgccgat cttggctgtg
    21781 gcccaggtaa cagcaccgcc cttctaaacc aacgttggcc tgctgccagg ataacaggca
    21841 tcgactcgtc tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg
    21901 tggaagcaga catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg
    21961 cctcactgca atggctgccc gaccactacg aattgtttcc tcatctggtt tctttactta
    22021 atccgcaggg cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc
    22081 tcatgcgcga agttgcctgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg
    22141 gcgttcatgc ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa
    22201 ccacctacta tcaccagatg ccgtcacacc aggcgattat cgattgggtg actgccactg
    22261 gattacgtcc gtggttacag gatctgaccg agagcgaaca gcagcttttt cttaagcgct
    22321 accatcagat gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat
    22381 ttccgcgtct ttttattgtt gcccggcgta tggagtaaat atcacgtcag ctggtaatga
    22441 cgatcgggaa gaatttttgc tggaatttcg gcttcatcgt tcatctgtaa caggtcaatt
    22501 tcaatagcgt tgcagatggc atccagtggt aaatcattgt tttcagtacc gaacggatct
    22561 tccagttctt ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata
    22621 aaaggcgtca tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga
    22681 taaacggtac gatgcaaaat cagggtgtag gcaaagggaa ttggcgtata ggcaatgcgc
    22741 tcgcatcctg ccaggaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg
    22801 atatctgaaa gctgcccatt gcggcgctga accgccaacc actcccccat tattaacaag
    22861 atacggttag ctggagagtt cgaagccagt acaggctgaa gatcttcagt cttgagataa
    22921 tgagccagca cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag
    22981 gcgatttgca accgggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc
    23041 tcgcgcagta acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg
    23101 taacgggcgt acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga
    23161 atgctgaacg gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaaa
    23221 ataacagcga tagaaaagag aaaatggagt agtaagcgcg atgatatctt ggataatact
    23281 gagccgtgcc agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt
    23341 atcttcaggc gtgaaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc
    23401 tatttacaaa ttacctacaa gcttacaaca ttaacaggcg ctgcatgtgg catctgttaa
    23461 tgataaaggc acataggatg ttgtaactaa tatagtgaaa taagaaaccc ggtcgaaacc
    23521 gggttcagaa gtaacggtgt tattagcaca acggacgtac agcttcgcgc atcccttttt
    23581 caagaatcgc atccaggtca ttagcaacct gctcgaccag accaggcact tgcgtcaggt
    23641 cctgctccca gtggtctttt tctgccaaca caatcgctac cagttcctgg gtaccgataa
    23701 cacggtcacg atgctggctc cagagttgct gataacgttc cagccagtgt gcatcgtcct
    23761 gtaccggata tgtttctccg ttacgctcac cacgatagaa cgcaatcaat gccgctaatg
    23821 cgaaagtaag gcgcgccgga agtgtgccgt ttgccttctg ccctgccagc agctgcggca
    23881 ggatgcgggt gcggaacttg gtcataccgt taagcgcgat agacagcagc tgatgcttaa
    23941 tgtacgggtt acggaaacgc ccggtgactg cactggcgaa agattccagt tcatcacgag
    24001 gcaaatccag taccggaata atttcttcgt agatagcttt ttcaacgaat gcgcaaattt
    24061 cagcatcgtt catcgcttca cctacggtat ccagccctgc ctggaacgcc accggcacca
    24121 gcgcggtgtg cgcaccgttg aggattgcca ctttgcgttc tttgtacggt ttaatatcgt
    24181 caacaatcag cacgttgagc ggatatttgt ccagacgcag ttcagtcgct aaggatttcg
    24241 gtccctgaat cacaaacagg taaaagtgtt cagcggtgtc gagaaaacca tcgtgataac
    24301 ccagctcttc ttccagttta gccacttcat cgcgcggata accggtaacg atacggtcca
    24361 ccagcgtaga acagaagctg ttagcctgat ccagccattg aataaatgct tctggcagtg
    24421 cccactcttg cgcatagcgc agcaccagtt cacgcaacgc gtcgccattg tagtcgatca
    24481 actcacacgg gatgatgatc caacctttat ccagcgcacc gttgaaatgg ctaaaacgtt
    24541 cgaacagcag acgggtcagt tttgccggat aacttaccgc tggcgcgtca tcgaatttat
    24601 cgcccgcatg gtagctgata cccgcttcgg tggtattaga gaaaacaaag cgcatttccg
    24661 ggttgtgagc cagtttcagg aattcatcgt attcactgta gacgctgatt tcacgattaa
    24721 ccgagcgaat cagacgcgcg tcgctgaccg cttccccctt ctcattcagg ccacggataa
    24781 tggtggtata cagaccatcc tgcgtgctca gtgacggtgg gaatgaagtt tcaatcggac
    24841 gaacaacgac cacaccagaa ttcagatcgg tgtgctcatt caggagatcg atttgccagt
    24901 caacaaaggc gcgcaggaag ttaccttcac caaactgaat gatacgttct ggatactgtg
    24961 caccgggaaa atcgcgacga tttagtgttt tcacaatggg ttcccttctg attagtcata
    25021 caacctgttt gaattggtac gacaggttag caaactttaa tacgccgaac ccctgttttg
    25081 atcaactcct gatgattaat gagcagtttt atgagaaaag tgtggcgcgg atcatggttt
    25141 aatcgaggaa atacgccttt tcctggatca taaagtggta gaacacattg cattcaaatc
    25201 gcgcggaatg aataaagatg tcagacaact tcctcaccgt aacgcatagt gctggtacgg
    25261 ttgcgcccat ctttcttcga gcgatacaga cacgtatcgg cttcgaccat caatttatta
    25321 aaatcatcgg tcagcgttcg gtacgatgcg cgaccactac cgacgccaat acttaccgtg
    25381 agataaagcg ttttttgttg ccaggtgaat ggttgcagtt caacgccttt acggattttt
    25441 tccgccatta gcagaccatc gacaggattc accgacggca ctgcaacagc aaattcttcg
    25501 ccgcccatgc gcgccaccag ccccttatca ccgacaatct tctgaatatg ccgggcaaac
    25561 acgcttaaca ctttatcgcc acattcatgt ccatagttat cgttaatgct tttgaaatag
    25621 tcaatatcaa gcagcatgac cgtcagatgt tgtgtctgtt tcagcgatgg acttttcagc
    25681 gcctcataaa gaccggaccg tgagtaaacc tgagtcagaa aatcaaagtc ggctcgcagc
    25741 gcaacttgct tcattagcga attgatcgct gccacgctaa aagaaaccat aattgggcat
    25801 atcgccatcg tggcaatacc gagacgtgcg gagaacattt ccggaatgga gaacggcgaa
    25861 ccgaccgaga tatcaatcac cgaatttgcc accagtacga tttccaccgc accggtgaca
    25921 aaggtcaaca gacatgttac ctgcggcgta tagcgcactg cacaccagat taatgcaggc
    25981 agcgggaacg ccagactccc cgctccgcca atgactaccg aggcaataac cgacacaata
    26041 agcgcgatgg cgggcatcat ctgttctgct ttaaagcgcg gcagcactcc aggaatagcc
    26101 aacgtcagca tacaaggcac gatcaacacg cccgttgaga attgctcact gaaccagtcc
    26161 gcaagcaaag gccagaaatc cagactatca atactgaccg aaccaatcgc cccgacaata
    26221 gcgcataata atgcggcaat cagacagtaa ttaaatagcc gtaaggcact tactggctca
    26281 tacttatttt tccccagacg cttatcccgc acgaccagta aggcgacggt aacaataaac
    26341 atcatattgg agaaattgat aaccagtgaa accagtcccc attcggtggt tatggcatca
    26401 taaaccagca tcgccacata actgatcgca tagtaatgca gtcgattaag ccagacatag
    26461 cgggcaaaca cccccgccat tacgccattc agcggccaga aaagagaaag ttcgtgaacc
    26521 agccgtaact ctgcaccaat aaaatagaac agcgtggtta gcgcaaaaat agcgatgcta
    26581 ttacgtagca gatgaccttc ctggaacaaa cggaaagttg aaatggggtg tacatgcatt
    26641 aattatctgt ccatacaatt gcgggtggca attatctcaa cgtaataagt aaattcacat
    26701 aaaggatagc atgttcgcta acagcggcgg caaataaagg taccctggga atgctatttt
    26761 atcgttttcc gcaattctca tggctgattc atctgtacaa ccagccagtg atagattacc
    26821 gccacgatat aacgttgttg ttcttcgctt aacgaaacac cctgcggaat gttatgccac
    26881 ttagccagac aaccgcgaca gcaggtcgcg gtggcgtgct gagcgataaa caccggatgc
    26941 ccgcgcatgg gggtttgctt accgtcatta gccggtaatg ccggagcaag acgtttagcg
    27001 acaaaatccg ccgcatgttg ttcgatgact ggcgcgcctt tctccaggca atactgacgc
    27061 tctttcatgc ccagacgaaa gcgagagcga aattttgaac gggataaacg cgcgaagagt
    27121 ggatcgagag actgcattaa taaaccgaac gccctaactg tttcgtcaat tgttcaagga
    27181 cggcgatacc cgcaagcgag ttacctgcat catccagttc cggactccag actgcgatgg
    27241 ccatttcatg cggaacaatc gccacaatac cgccaccaac gccagatttc gccggtagtc
    27301 ccacccgcca ggcaaactcc cccgcgttct ggtacatacc actggtcgcc atcagcgcgt
    27361 taatttgccg cgcctgcatt ggcgtcacca caggttcatc aatatgaata gctttcccct
    27421 gattagccag aaagacaaac gtccgggcca gctctacaca gctcattttc agagcgcagt
    27481 aatgaaagta gttttgcaga acggttgtca cgtcatgatg gaaattgcca aacgacttca
    27541 tcagccaggc gatagccgca tttcgcgcgg aatgttcaaa ttcggaacgc gctaccaccg
    27601 tatcgtatga aatatcagac acaccgctta agccgcgcac gacttccagc atacgttgcc
    27661 gtggtgcgct tagtcgccct tgcaacatat cgcagaccac cagcgcaccg gcattaatga
    27721 acggattacg cggaataccc tgttccattt ccagttgcac taaagaattg aacggtgatc
    27781 cagacggatc tttgccgacg cgttgccaga tttcctcttc ggagtaatga cgcatggcga
    27841 caacgagact cagcactttg gaaatagact gaatggaaaa acgttcttgc gcgtctccgg
    27901 cctgaaaaag ctgtccgtca acggtacaga tagcaatccc caatcgggaa ccgtctactg
    27961 tagccagcgc cggaatataa tccgcgactt taccctgacc aatgagcggc cgcacttgcc
    28021 gcaagatgtt ttctaaaatt gcattatcca tggcgactgc cactttctac tcctggaccg
    28081 caggtctgaa aagacctgcg agtatatcag agctgaatat gtcgcgtcag atccggtctt
    28141 tccacaccgt ctggatatta cagaattcgt gtaagccgaa atgggaaagc tcacgaccaa
    28201 agccactctt tttcacgcca ccaaaggcca ctcgcgcgtc gctggcacaa taaccattga
    28261 taaacacccc accgcattcc agacgtgccg ccatctgtct ggcctgtgtt tcgtcggtgg
    28321 taaaaatggt cgctgaaagg ccgaactcac tatcattagc cagttccagc gcatgttctg
    28381 catctttcgc aacggtgatt gccgcaacag ggccaaacat ttcttcccga aacgcggtca
    28441 tttctggggt aacattcgcc agaaccgttg gcggatagta gttacctgcc ccagccatct
    28501 tttccccgcc cagtaacaaa cgcgcaccct gcgccagggt tttctccacc tgatgatgca
    28561 gctcatcacg taaatcaaaa cgagccattg gtccgagagc gttctcttca tcacggggat
    28621 cgcccatttt caaggctgcc gcagctgcca caaaacgttc ggtaaatgca gaagcaattc
    28681 cctcttcgat aataaagcgt tttgccgctg cacaaacctg tccggtattc tgataacgtc
    28741 cggctaccgc cgctttaacc gccagttcca gatcggcatc gttaagcaca ataaatggat
    28801 ccgaaccgcc cagttccagt acgcattttt tcagtgccgc tccagcctgt gcgccaatag
    28861 ccgctcccgc acgaacactt ccggtcaccg tgacagcagc aatgcgcgaa tctttaatca
    28921 tttgactgac accgtcgttg tcggcattca gccagccata tacgccttgc gggattcccg
    28981 catctttaaa cacctgggca atgagctgag cacagcccat cacattcggc gcatgtttaa
    29041 gtaagtagcc gttacctgca agaatgatgg gaaccgcgcc acgcatcacc tgccataacg
    29101 gaaagttcca cggcataatc gccagaatcg tccccaacgg tcgatactca ataactgcct
    29161 gctgattttc caccagcgta ggttccgcct tcagcattgc cggaccatgt tctgcatacc
    29221 agtcacacaa attcgccgat ttcgccactt cagcgcgcgc ctgattgatt ggtttgccca
    29281 tttcacgggt gatcatttgc gccatttctt cgctacgggc gcgcagagcc ttaccgatac
    29341 cacgcagttt ttcagcacga taatctatat ttgtctcgcg ccagtcgcga aagcctgctg
    29401 ccgccagctg aagtgcgttt tcgatatcgt tagcgccagc ccacggcagc acagaaagtt
    29461 gttcacccgt ggcaggattt attgaaattg catgagttgc cggagtaatg gtcatcgggg
    29521 tatctccttt atgagtcatg gtatgaagat acgcagattt actcttgctt taaaatgaat
    29581 aatattaagc cacttattca cgaatcgaga atattatgga tctgacacaa ctggagatgt
    29641 tcaacgcggt tgccgaagct ggcagcataa cccaggcagc agcaatagtg catcgcgtgc
    29701 cgtcgaatct cactacccgt ctacgccagc tggaaacaga actgggggta gatctgttta
    29761 ttcgcgagaa tcagcgttta cgtctctctc ctgccgggca taacttttta cgctatagcc
    29821 aacaaattct cgcgttagtg gatgaagcgc ggagcgttgt cgctggcgat gagccgcaag
    29881 gtttattttc tcttggttcg ctggaaagca ccgcagcagt gcgcattccg gccacgctgg
    29941 cggaattcaa ccatcgttat cccaaaattc agttttcact ttccaccggc ccttccggca
    30001 ctatgctgga aggagtgctg gagggaaaac tgaatgcggc atttattgat gggcccatta
    30061 accatactgc cattgacggg ataccggtat accgcgagga gctgatgatc gtcacgccac
    30121 aaggtcatgc gccagtaatc cgcgccagtc aggttaacgg cagtaacatt tatgccttcc
    30181 gcgccaattg ttcgtatcgt cgccacttcg agagctggtt tcatgctgac ggtgccgctc
    30241 cgggaactat tcacgagatg gagtcgtatc acgggatgtt ggcctgtgtg gtcgcaggag
    30301 caggcattgc gcttattcca cgctctatgc tggaaagtat gccggggcat caccaggttg
    30361 aagcgtggcc gttagctgag caatggcgtt ggttaacaac ctggctggtc tggcgtcgcg
    30421 gtgcgaaaac gcgtccgctt gaggcattta ttcaactact ggatgtgtct gactcggcaa
    30481 aacagagtta ccaatgagct attttgatag ttcttgcggc taatatgctc tatatagtga
    30541 tgttccgatg acttatgact atatggggca aatatggtta cgccagtaag catcagcaat
    30601 tacatatctc ttcctgatga ttttcccgtt cgtaatattg caccacaggt aaaagaagtt
    30661 ttaaaggact ttattgatgc gctaagtaca ataatatgtg atgaagagtg gcgcacgtct
    30721 ttaaacatca actcagccac aaaaaagata tttaataacc ttgataactt atcatatatt
    30781 cagaggactt cttttcgggg taacgacacg ctatacaatg aaaaggttca gtttaaactc
    30841 acttatcccg tcaagaatgg aagacacaaa gaaaatattg aatttcaggt agtaataaat
    30901 ttaagcccta tttatttaga taattttcgc catgatggag aaattaatat ttttgcgctc
    30961 ccaacccaaa gcctgtcact atggggcgcg tatttcagac cggcgtcgag cgtgtgctat
    31021 ttctgtttct gaatgatttt attgaacaat ttccaatgtt caaccttggc gttcccatca
    31081 aaagagcgca tacaccacat attgaacccc tgcctccgga tcgccatacc gctgcagatt
    31141 acttacgcca gtttgatttg cttgtcctga attttatctc tcgcggtaat tttgtcatac
    31201 tcccccgatt atggaataac tctgaggttc acagatggtt cgtcaataaa gatcctaact
    31261 tgatcaccgc cattctcgac ataacggaca gcgaattaaa agaggattta ttgcaaagcc
    31321 tgatggattc attaggttct aacaaacatg tactacccga agtctgcatc tgctttttat
    31381 cccttttagc agaacaagaa tctcctcatt ttcaggactt gtttttattt ttcgccaata
    31441 tgttattgca ctatcaccaa tttatgaatc ccaatgaaag tgatttgaat gacgtgttga
    31501 tgccagcatc attaagtgat gataaaatta tcaaacatat ggcgcgcagg accctcaaac
    31561 tgtttgtaaa aaatgaaacg ccgccaaaag ttactcacga agacctggtg aaaaacaggc
    31621 ctcgctcccc tgtcagacca cctatacccg caaccgccaa aacgccagac ctccctgaac
    31681 gtcattaaac cgtgatgtta ccgactctct gacgcggaac agaatcagcg tcagagaaac
    31741 ggaaaacgcg atccagatca caaatgcatt gtattcacat cattaaccgt tttaagatca
    31801 tttcatcact ttttcgcaac tcacccgata atctgttatg acaacaaaca ctgtttcccg
    31861 caaagtggcg tggctacggg tcgttacgct ggcagtcgcc gccttcatct tcaacaccac
    31921 cgaatttgtc cctgttggcc tgctctctga cattgcgcaa agttttcaca tgcaaaccgc
    31981 tcaggtcggc attatgttga ccatttatgc atgggtagtg gcgctaatgt cattgccttt
    32041 tatgttaatg accagccagg ttgaacggcg caaattactg atctgcctgt ttgtggtgtt
    32101 tattgccagc cacgtactgt cgtttttatc gtggagcttt accgttctgg tgatcagtcg
    32161 cattggtgtg gcttttgcac atgcgatttt ctggtcgatt acggcgtctc tggcgatccg
    32221 tatggctccg gccgggaagc gggcacaggc cttgagttta attgccaccg gtacagcact
    32281 ggcgatggtc ttaggtttac ctctcgggcg cattgtgggc cagtatttcg gttggcgaat
    32341 gaccttcttc gcgattggta ttggggcgct tatcaccctt ttgtgcctga ttaagttact
    32401 tcccttactg cccagtgagc attccggttc attgaaaagc ctcccgctat tgttccgccg
    32461 cccggcattg atgagcattt atttgttaac tgtggtggtt gtcaccgccc attacacggc
    32521 atacagctat atcgagcctt ttgtgcaaaa cattgcggga ttcagcgcca actttgccac
    32581 ggcattactg ttattactcg gtggttcggg cattattggc agcgtgattt tcggtaaact
    32641 gggtaatcag tatgcgtctg cgttggtgag tacggcgatt gcgctgttgc tggtgtgcct
    32701 ggcattgctg ttacctgcgg cgaacagtga aatacacctc ggggtgctga gtattttctg
    32761 ggggatcgcg atgatgatca tcgggcttgg tatgcaggtt aaagtgctgg cgctggcacc
    32821 agatgctacc gacgtcgcga tggcgctatt ctccggcata tttaatattg gaatcggggc
    32881 gggtgcgttg gtaggtaatc aggtgagttt gcactggtca atgtcgatga ttggttatgt
    32941 gggcgcggtg cctgcttttg ccgcgttaat ttggtcaatc attatatttc gccgctggcc
    33001 agtgacactc gaagaacaga cgcaatagtt gaaaggccca ttcgggcctt ttttaatggt
    33061 acgttttaat gatttccagg atgccgttaa taataaactg cacacccata cataccagca
    33121 ggaatcccat cagacgggag atcgcttcaa tgccaccctt gcccaccagc cgcataattg
    33181 cgccggagct gcgtaggctt ccccacaaaa taaccgccac caggaaaaag atcagcggcg
    33241 gcgcaaccat cagtacccaa tcagcgaagg ttgaactctg acgcactgtg gacgccgagc
    33301 taataatcat cgctatggtt cccggaccag cggtacttgg cattgctagc ggcacaaagg
    33361 caatattggc actgggttca tcttccagct cttccgactt gcttttcgcc tccggtgaat
    33421 caatcgcttt ctgttgcgga aagagcatcc gaaaaccgat aaacgcgacg attaagccgc
    33481 ctgcaattcg cagaccggga atcgaaatgc caaatgtatc catcaccagt tgcccggcgt
    33541 aatacgccac catcatgatg gcaaatacgt acaccgaggc catcaacgac tgacgattac
    33601 gttcggcact gttcatgttg cctgccaggc caagaaataa cgcgacagtt gttaatgggt
    33661 tagctaacgg cagtaacacc accagcccca ggccaattgc tttaaacaaa tctaacattg
    33721 gtggttgtta tcctgtgtat ctgggttatc agcgaaaagt ataaggggta aacaaggata
    33781 aagtgtcact ctttagctaa ccttgcatcg cattgaccaa aacttgaacc gatttagcaa
    33841 aacgtggcat cggtcaattc attcatttga cttatacttg cctaggcaat attatcccct
    33901 gcaactaatt acttgccagg gcaactaatg tgaaaagtac cagcgatctg ttcaatgaaa
    33961 ttattccatt gggtcgctta atccatatgg ttaatcagaa gaaagatcgt ctgcttaacg
    34021 agtatctgtc tccgctggat attaccgcgg cacagtttaa ggtgctctgc tctatccgct
    34081 gcgcggcgtg tattactccg gttgaactga aaaaagtgtt gtcggtcgac ctgggagcac
    34141 tcacccgtat gctggatcgc ctggtctgta aaggctgggt tgaaaggttg ccgaacccga
    34201 atgataagcg cggcgtactg gtaaaactta ccaccagcgg cgcggcaata tgcgaacaat
    34261 gccatcaatt agttggccag gacctgcatc aagaattaac aaaaaaactg acggcggacg
    34321 aagtggcaac acttgagcat ttgcttaaga aagtcctgcc gtaaacaaaa aagaggtatg
    34381 acgatgtcca gacgcaatac tgacgctatt accattcata gcattttgga ctggatcgag
    34441 gacaacctgg aatcgccact gtcactggag aaagtgtcag agcgttcagg ttactctaaa
    34501 tggcacctgc aacggatgtt taaaaaagaa accggtcatt cattaggcca atacatccgc
    34561 agccgtaaga tgacggaaat cgcgcaaaag ctgaaggaaa gtaacgagcc gatactctat
    34621 ctggcagaac gatatggctt cgagtcgcaa caaactctga cccgaacctt caaaaattac
    34681 tttgatgttc cgccgcataa ataccggatg accaatatgc agggcgaatc gcgcttttta
    34741 catccattaa atcattacaa cagctagttg ataacgtgac aacgtcactg aggcaatcat
    34801 gaaaccactt tcatccgcaa tagcagctgc gcttattctc ttttccgcgc agggcgttgc
    34861 ggaacaaacc acgcagccag ttgttacttc ttgtgccaat gtcgtggttg ttcccccatc
    34921 gcaggaacaa ccaccgtttg atttaaatca catgggtact ggcagtgata agtcggatgc
    34981 gctcggcgtg ccctattata atcaacacgc tatgtagtta ttgtcaggct ctgatcacag
    35041 agcctgacat ctccgtaata tcttcctcaa gtctcaaata ttttcgccat tgttggcgat
    35101 gagatcccga taccagtaaa acgacgcttt acgtatccgt tccagcgttc ccttcccttc
    35161 gttgtcttta tcgacataaa tcattccgta gcgctttttc atttcccctg ttccggcaga
    35221 aaccaggtca atgcagcccc acggggtgta gccaattaag tcaacaccat cttcaactac
    35281 ggcttttttc atttcccgaa tatgggaagc aaagtaatca atgcgatagt gatcgttcac
    35341 cgtgccgtca gcttgtctct ggtcaaccgc accaaatcca ttttcgacaa taaacagcgg
    35401 caactggaaa tgatcccaga accagttgag tgaataacgt agccctgccg gatcaatttg
    35461 ccatccccat tcggatgtat cgatataagg gttgctgacc aaatctcgtg gttcaacata
    35521 atccagttgc gggttatcgg gcgaaaattg tgtcgtaaaa gacatgtagt agctaaagcc
    35581 gataaagtcg acacaaccac tggcaagaat cgcgttatct tctggtgtga tatcgaggtt
    35641 gaatcctttc ctggcaaagt aattcagcat atgttgcgga taatatccac gagtatgaac
    35701 atcagtaaac cagtaacgac gatgcattgc tttcgtggcc atcatcatat cgttgggtgc
    35761 acacgtcaga ggatagatgg gacacatggc gatcatacag ccaatgttaa attcaggatt
    35821 gatcgatttt ccagtctgta ccgccagggc actggcaact aactcgtaat gtaccgcctg
    35881 gtacattatt tgttcgcgct cattgatatc ttcctctggc gaatacaaga taccggaatt
    35941 agtaaatgga cacaggcttt cgctgaaatt cacctgatta ttgatttcgt taaacgtcat
    36001 ccagtacttt actttttctt tatagcgcgt gaagaccgtt gatgcgaagc ggataaaaaa
    36061 gtcgatcagt ttacggtttc gccagccacc atattttgtc accagatgat aaggcatctc
    36121 aaaatgtgaa agcgtcacca caggttccat tccctgctta aggcattcat cgaacagatc
    36181 atcataaaat tgtaaaccct cttcattcgg ctcctgttcg tcaccttgcg gaaagattcg
    36241 tgtccaggca atggaagttc gaaagcattt gaatcccatc tcggcaaata actgaatatc
    36301 tgttttatag cgatgataaa aatcaattgc ttcatgattg ggataattaa gcccgtcgat
    36361 aacgccttct gtcacttcac gcggcacccc gtgagcgcca gcagtcatca catcagcgat
    36421 gctgatgcct tttcctcctt cattccagcc accttccaac tgatgcgcgg ctaccgcgcc
    36481 gccccataaa aaaccttttt taaatcctga catacacaac tccttaacta aaatagatta
    36541 ccaccagact tccgcctgga tgccggccat aaactcgtct ttgctgttct cattaaactt
    36601 aaaattcgat aattcgttat ccagaatatt gatatacgtt ccatagaagc gaatttctgg
    36661 tcgtgaacct aaaatgcttt cacccacttt caatgcatgc cagagtgtcg ttttataagc
    36721 cgattcattt agtgttacac cctgctgagt tttgttctgt tgcttaaacc agcctaattc
    36781 aagccccgtc tgattccatg tattccagat ccaggccggt cttattacag tgcgaatact
    36841 gtcaaaatca ctatgagcac cactttcata actataaaca tcttcgccgt gagaatagac
    36901 aagcgcgtta gccataataa tattgtcagt aagatacatc tcgccctgag agattaaacg
    36961 ccaggcgatc ccattggtat gatcaccata gtaatagcga ccatgcgcca tcgtattact
    37021 ggcatctgag aaactggcaa aactgctggc ataggaatta ttggcaacct gtaacgtaaa
    37081 ttcattaaac gtatcgcgtt gcaggttttg ccgtaaaaca gaagtcagca tccagctatc
    37141 tttcatttca aaataactgt tgtcattttc gttatcttgt tgttgatccg ttttattagg
    37201 tgcggaatat ttagccatta atgataatgt cgcatcatcc cataacggga tattgcgata
    37261 acgaacatcc acagaattgg tattcatctg gctggtacgc gaaaaatcac gagagtaaac
    37321 atcgacatca tcacggctta aggacatatc aaacagccct acaccaagtg cccagttttc
    37381 aatccccaca cccgcggcga catccgtggt taaggttttc cagtccagca tctggatctc
    37441 atattgcggg agtttatgtt tgccgaccca gaagtctgcc tctggtgcga agggtaaaaa
    37501 acctcgcgtt gtcaggtaaa tatcgctgaa ctgcatgata ttttcattgg cactgtcacc
    37561 aaaccaggca tcgttatact gctcacccac gttgccatca taggtaacga ccgcatttgc
    37621 cgttttaccg tcctgattat aaacacgctg atttaaggtc aggtcaaacc aaccgctcat
    37681 ctcattacca aaacgtccca gagaacccgc tgcataagtt tgtggtgagc cgtgattacc
    37741 agtaccccag cctgagcgga agtacccctg atagctgaac ccaatatcat cctttatata
    37801 tttactgata tctttcaggg tcacgctttc gatagtggtt ttgtcgcctt tactttcaat
    37861 aacggcaact tgctgcgatc catttatagt atgcgctgcg gtaaacgagt tctgttcacc
    37921 gttttcattt ttgatgttat cagcgacggg agatgtcgct gatatttcag caagcgaggc
    37981 tgatttatat ttgttttccg tgatgctttt ttgtaaggtc gaaagtcggg atttatatac
    38041 tcccagttca ttctgtgttg ctttcagctc ttgtttattt tgcgacaatt cattttcaag
    38101 caattcaagg cgctgctcta tggttaattt tgttgccatc gcgttcaatg gaaacaatgc
    38161 tgtaataata gaaaaagaca aaaggctgac gttaagcgtc tttatattca tgacttatcc
    38221 ttaagagtat aataatttcc tccagctatc gcactggagg aaaaaatcag gcatggagtt
    38281 cttttcttaa tgaaataatt tccctggcta aatcctggca taatattgca ttcatcaaat
    38341 gatcctgtgc atgaatcaga attaaattaa cttttatttt gccacagcct tcatcctgac
    38401 taattaatgc ggtctgaatc ttatgtgcct gaagacaggc agattcagaa ctggccatta
    38461 gctgagttgc gccctgccag tctttttgcc cggcacaacg tatggcttcc atcgcgtcag
    38521 aacgtgcctg ccccgcgttg accagtaact ccataacaag ttcttcatct gcaaacatca
    38581 caatctcctt cctgggtaaa tggaattatc cgttagttgt catgttattg actttgttgg
    38641 cggcaataac aaaaggtaaa tagatcattg tcgccacacc taaacataaa aaaccaacca
    38701 gtaaagccag ccagttcccc ccggttccga ggaaagcaat taaaggtccg ggcgtagtcc
    38761 aaggcacggc ataagcaacc ggtggaataa tttccatcga aacaaagaag taaccaatgg
    38821 cacagttaac aatgggaacc ataataaacg gcaccatcat gatggggtta agtacaatag
    38881 gaaggccaaa cattatcggt tcattgatat tgaaaatacc ggggataaat gacatttttg
    38941 ccagatcacg gtattccgca cgcttagaag cgataaaaat agccaacaat aaccccaacg
    39001 tcatacctga accgccgcag ttggcgaatg catcattaat agaagtccag gtaatcggat
    39061 atggcgcgcc ccaggtagtg ccatgttgag cggcccagga gagattctcc agattagcct
    39121 cagaaaaaac ggtttctcga attgccgatg tggtattagg tccgtggatc cccagcaccc
    39181 acaggaaatt acccacagcc cccaggataa ttaccgcaaa gatattcgtt cccatatgtt
    39241 tgagcggcgt ctggattaat gtgtaaataa gatcgtttag tcctgccggg gatatcagtg
    39301 tcaggcaata attaagtgcg gaaaagaaca ccatgacaaa aaatattggc aataaaactt
    39361 taaatgaacg cgctaccgca ggcggtacag ctgccggcat cgttatggtg atttttgggt
    39421 ttcgagctaa gcgacaaaat atttcactgg tgatcaatgc aacgataaca gcaacaaaca
    39481 acccctgcgc gccgagatat ttggtcgtca ggaatgcttg cccatctatc atctgatatg
    39541 gcgtataaac aataaaaaat gctcctattg ccgttaatcc gcataaaaga tcatcttgct
    39601 catagctaat cgccatattc ctggcgacca ggaaagcaat cataattgac atgatattta
    39661 cagaaccatt cattaccgga gtaaaaatag cttgtgcttt ttcaaggttg gggaaaatgc
    39721 tattcagatg cagtaatccg gcaataaaac cgtctggcga taatatggca aagttaatta
    39781 atataattaa tgaacttgcc atgataattg gaaacgtaaa aacgaatcca tctctcaccg
    39841 ctgcaacatg tttttgtgag tttaacttga tagcaactgg aacaagaaaa cgttccattc
    39901 cacgttcgaa tgaggccatt aatcccattt ttttatacct gttgttttgg atatttatac
    39961 atcccaataa agcatgtatg ccatatccat tttatgatgt gtgaaaagtt agttcaggta
    40021 atagtttaat aatattagat tcttactctt gcagtagttt catcgcaaat tcaagaactg
    40081 cctgtccgtt catggtgccg tagtctttca tctcgataac ggctacaggc attttcccct
    40141 gtgcaacggc ttcaatctct ggtttctgaa agcgaacttg cgggccaagt aaaacaacgt
    40201 caacttcgtt atttttaatt ttccctttag cctctgcaat cgccaatgcg gaaatattaa
    40261 cttcaagtga aatagcgtta gcatgatcaa tcatacgttt aaccagcata ctggttgaca
    40321 tgcccgcagc gcaaactaac aatatttttt tcatattttc ccttactggt atataacaga
    40381 ttacattgtt taaatatcat atcccgcagt agctatacgt cctggtacat tgatcacagt
    40441 aatacagtga taaagttgtt caacatcagg gtgattatta aacgactcgg ccatacgata
    40501 aaccaatcag aactcaactc cttgttttca atggctctta aaaggtgcaa aagtgatttt
    40561 tcaaaagatt gcccgcttac ttaaatccga aatcaatggc aattcatggc atgttggtga
    40621 tttgttgccg tcagaagcgg aactcgctgt tcgttataat gtttcgcgta ataccctgcg
    40681 taaggcatta tccttgctgg aaggcgaagg aattattcac agaaagcatg gttcaggaac
    40741 atacattcaa aaaaagaatt ttgttgcaca cattgatcac atgaacagtt tcagtgaaat
    40801 tgcgcataaa agcggcaaag aggcagggag tcagattatg aaatttgaag tgcaggatgc
    40861 ttcacccact attgcgactg agctgaattt agtgactggt gagcaggttt attacataaa
    40921 gagactgcga ttcattgagg ataatgcagc acaattagaa gaaacgtgga tgtcagtagc
    40981 acgttttcct gatttaaccg tgtcacatat gcaaaaatcc aaattttcat atattgagaa
    41041 tgaatgcggg atcaaaatca ttggcacctt tgaaactttc tcccctactt ttcctacccc
    41101 agaaatcgcc agtattttac ggatcagccc acgggatccc atacttaaaa ttcagaccca
    41161 ggctgtggac agtaactcta ttccgctgga ttattcgtta ctttacagca atattttcga
    41221 gttccaggta aagtactttt tcccgcgata aaagcagacg cacagagtgc tgacatcaca
    41281 ttctgtgcgt atggtttagc ctctaacctt taccgcttta cgccaccgca agccaaatac
    41341 attgatatac agcccggtca taatgagcac cgcacctaaa aattgcagac ccgttaagcg
    41401 ttcatccaac aatagtgccg cacttgccag tccgactacg ggcaccagta acgataacgg
    41461 tgcaacccgc caggtttcat agcgtcccag taacgtcccc cagatcccat aaccaacaat
    41521 tgtcgccaca aacgccagat acatcagcga caagatggtg gtcatatcaa tagtaaccag
    41581 actgtgaatc atggatgcgg aaccatcgag aatcagcgag gcaacaaaga agggaatgat
    41641 tgggattaaa gcgctccaga ttaccagcga catcaccgcc ggacgcgttg agtgcgacat
    41701 gatcttttta ttgaagatgt tgccacacgc ccaactaaat gctgcagcca gggtcaacat
    41761 aaagccgagc atcgccacat gctgaccgtt cagactatct tcgattaaca ccagtacgcc
    41821 aaaaatcgct aaggcgatcc ccgccaattg tttgccatgc agtcgctccc cgaaagtaca
    41881 cgcgccaagc acgatagtaa aaaacgcctg tgcctgtaac accagcgaag ccagtccagc
    41941 aggcatacca aagttaatgg cgcagaacag aaaggcaaac tgcgcaaaac tgatggttaa
    42001 tccatacccc aagagcaaat tcagtggtac tttcggtcgt gcaacaaaaa agatggccgg
    42061 aaaagcgacc agcataaagc gcaaaccggc cagcatcagc ggtggcatgt tatgaagccc
    42121 cactttgatg accacaaaat ttagccccca tacgaccact accagtagcg ccaacacccc
    42181 atcttttcgc gacattctac cgcctctgaa tttcatcttt tgtaagcaat caacttagct
    42241 gaatttactt ttctttaaca gttgattcgt tagtcgccgg ttacgacggc attaatgcgc
    42301 aaataagtcg ctatacttcg gatttttgcc atgctatttc tttacatctc taaaacaaaa
    42361 cataacgaaa cgcactgccg gacagacaaa tgaacttatc cctacgacgc tctaccagcg
    42421 cccttcttgc ctcgtcgttg ttattaacca tcggacgcgg cgctacgctg ccatttatga
    42481 ccatttactt gagtcgccag tacagcctga gtgtcgatct aatcggttat gcgatgacaa
    42541 ttgcgctcac tattggcgtc gtttttagcc tcggttttgg tatcctggcg gataagttcg
    42601 acaagaaacg ctatatgtta ctggcaatta ccgccttcgc cagcggtttt attgccattc
    42661 ctttagtgaa taacgtgacg ctggtggtgc tcttttttgc cctcattaac tgcgcctatt
    42721 ctgtttttgc taccgtgctg aaagcctggt ttgccgacaa tctttcgtcc accagcaaaa
    42781 cgaaaatctt ctcaatcaac tacaccatgc tcaacattgg ctggaccatc ggtccgccgc
    42841 tcggcacgct gttggtgatg cagagcatca atctgccctt ttggctggca gctatctgtt
    42901 ccgcgtttcc catgcttttc attcaaattt gggtaaagcg cagcgagaaa atcatcgcca
    42961 cggaaacagg cagtgtctgg tcgccgaaag ttttattaca agataaagca ctgttgtggt
    43021 ttacctgctc tggttttctg gcttcttttg taagcggcgc atttgcttca tgcatttcac
    43081 aatatgtgat ggtgattgct gatggggatt ttgccgaaaa ggtggtcgcg gttgttcttc
    43141 cggtgaatgc tgccatggtg gttacgttgc aatattccgt gggccgccga cttaacccgg
    43201 ctaacatccg cgcgctgatg acagcaggca ccctctgttt cgtcatcggt ctggtcggtt
    43261 ttattttttc cggcaacagc ctgctattgt ggggtatgtc agctgcggta tttactgtcg
    43321 gtgaaatcat ttatgcgccg ggcgagtata tgttgattga ccatattgcg ccgccaggaa
    43381 tgaaagccag ctatttttcc gcccagtctt taggctggct tggtgccgcg attaacccat
    43441 tagtgagtgg cgtagtgcta accagcctgc cgccttcctc gctgtttgtc atcttagcgt
    43501 tggtgatcat tgctgcgtgg gtgctgatgt taaaagggat tcgagcaaga ccgtgggggc
    43561 agcccgcgct ttgttgattt aagtcgaaca caataaagat ttaattcagc cttcgtttag
    43621 gttacctctg ctaatatctt tctcattgag atgaaaatta aggtaagcga ggaaacacac
    43681 cacaccataa acggaggcaa ataatgctgg gtaatatgaa tgtttttatg gccgtactgg
    43741 gaataatttt attttctggt tttctggccg cgtatttcag ccacaaatgg gatgactaat
    43801 gaacggagat aatccctcac ctaaccggcc ccttgttaca gttgtgtaca aggggcctga
    43861 tttttatgac ggcgaaaaaa aaccgccagt aaaccggcgg tgaatgcttg catggataga
    43921 tttgtgtttt gcttttacgc taacaggcat tttcctgcac tgataacgga tcgttgacac
    43981 agtagcatca gttttctcaa tgaatgttaa acggagctta aactcggtta atcacgtttt
    44041 gttcgtcaat aaacatgcag cgatttcttc cggtttgctt accctcatac attgcccggt
    44101 ccgctcttcc aataaccaca tccagaggct cttcaggaaa tgcgcgactc acacctgctg
    44161 tcacggtaat gttgatatgc ccttcagaat gtgtgatggc atggttatcg actaactggc
    44221 aaattctgac acctgcacga catgcttctt catcattagc cgctttgaca ataatgataa
    44281 attcttcgcc cccgtagcga taaaccgttt cgtaatcacg cgtccaactg gctaagtaag
    44341 ttgccagggt gcgtaatact acatcgccga ttaaatgccc gtaggtatca ttaaccaatt
    44401 taaatcggtc aatatccaac aacattaaat aaagattcag aggctcagcg ttgcgtaact
    44461 gatgatcaaa ggattcatca agaacccgac gacccggcaa tcccgtcaaa acatccatat
    44521 tgctacggat cgtcagcaaa taaattttgt aatcggttaa tgccgaagta aaagaaagca
    44581 acccctcctg aaaggcgtcg aaatgcgcgt cctgccagtg attttcaaca atagccagca
    44641 ttaattcccg accacagtta tgcatatgtt gatgggcaga atccattagc cgaacgtaag
    44701 gtaattcatc gttatcgagt ggccccagat gatcaatcca ccgaccaaac tggcacagtc
    44761 cataagaatg gttatccgtt atttctggct tactggcatc tctcgcgacc acgctgtgaa
    44821 acatactcac cagccactgg tagtgggcat cgatagcctt attgagattt aacaagatgg
    44881 catcaatttc cgttgtcttc ttgatcattg ccactccttt ttcacagttc cttgtgcgcg
    44941 ctattctaac gagagaaaag caaaattacg tcaatatttt catagaaatc cgaagttatg
    45001 agtcatctct gagataacat tgtgatttaa aacaaaatca gcggataaaa aagtgtttaa
    45061 ttctgtaaat cacctctgca ttatcgtaaa taaaaggatg acaaatagca taacccaata
    45121 ccctaatggc ccagtagttc aggcc
//
LOCUS       DAZFXT010000023        45032 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.023, whole genome shotgun sequence.
ACCESSION   DAZFXT010000023 DAZFXT010000000
VERSION     DAZFXT010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45032
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.023"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1861)
                     /gene="htpG"
                     /locus_tag="ACHKW0_001413"
     CDS             complement(<1..1861)
                     /gene="htpG"
                     /locus_tag="ACHKW0_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HFO7474275.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDADKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQ"
     gene            complement(1971..2576)
                     /gene="recR"
                     /locus_tag="ACHKW0_001414"
     CDS             complement(1971..2576)
                     /gene="recR"
                     /locus_tag="ACHKW0_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HFO7474276.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            complement(2576..2905)
                     /gene="ybaB"
                     /locus_tag="ACHKW0_001415"
     CDS             complement(2576..2905)
                     /gene="ybaB"
                     /locus_tag="ACHKW0_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HFO7474277.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            complement(2958..4889)
                     /gene="dnaX"
                     /locus_tag="ACHKW0_001416"
     CDS             complement(2958..4889)
                     /gene="dnaX"
                     /locus_tag="ACHKW0_001416"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HFO7474278.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDAWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSTLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    complement(3561..3625)
                     /gene="dnaX"
                     /locus_tag="ACHKW0_001416"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            complement(5018..5569)
                     /gene="apt"
                     /locus_tag="ACHKW0_001417"
     CDS             complement(5018..5569)
                     /gene="apt"
                     /locus_tag="ACHKW0_001417"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HFO7474279.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            complement(5722..6099)
                     /gene="ybaN"
                     /locus_tag="ACHKW0_001418"
     CDS             complement(5722..6099)
                     /gene="ybaN"
                     /locus_tag="ACHKW0_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HFO7474280.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            6169..6696
                     /gene="priC"
                     /locus_tag="ACHKW0_001419"
     CDS             6169..6696
                     /gene="priC"
                     /locus_tag="ACHKW0_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HFO7474281.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            6710..6871
                     /gene="rsmS"
                     /locus_tag="ACHKW0_001420"
     CDS             6710..6871
                     /gene="rsmS"
                     /locus_tag="ACHKW0_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HFO7474282.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(6924..7022)
                     /locus_tag="ACHKW0_001421"
                     /pseudo
     CDS             complement(6924..7022)
                     /locus_tag="ACHKW0_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(7083..10445)
                     /gene="mscK"
                     /locus_tag="ACHKW0_001422"
     CDS             complement(7083..10445)
                     /gene="mscK"
                     /locus_tag="ACHKW0_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HFO7474283.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(10573..11220)
                     /gene="acrR"
                     /locus_tag="ACHKW0_001423"
     CDS             complement(10573..11220)
                     /gene="acrR"
                     /locus_tag="ACHKW0_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HFO7474284.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            11362..12555
                     /gene="acrA"
                     /locus_tag="ACHKW0_001424"
     CDS             11362..12555
                     /gene="acrA"
                     /locus_tag="ACHKW0_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HFO7474285.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKARPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            12578..15727
                     /gene="acrB"
                     /locus_tag="ACHKW0_001425"
     CDS             12578..15727
                     /gene="acrB"
                     /locus_tag="ACHKW0_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease AcrB"
                     /protein_id="HFO7474286.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            16273..16647
                     /gene="tomB"
                     /locus_tag="ACHKW0_001426"
     CDS             16273..16647
                     /gene="tomB"
                     /locus_tag="ACHKW0_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HFO7474287.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            16673..16891
                     /gene="hha"
                     /locus_tag="ACHKW0_001427"
     CDS             16673..16891
                     /gene="hha"
                     /locus_tag="ACHKW0_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HHA domain-containing protein"
                     /protein_id="HFO7474288.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            17063..17614
                     /gene="maa"
                     /locus_tag="ACHKW0_001428"
     CDS             17063..17614
                     /gene="maa"
                     /locus_tag="ACHKW0_001428"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HFO7474289.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            17730..18200
                     /gene="ylaC"
                     /locus_tag="ACHKW0_001429"
     CDS             17730..18200
                     /gene="ylaC"
                     /locus_tag="ACHKW0_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HFO7474290.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            18364..19914
                     /gene="pdeB"
                     /locus_tag="ACHKW0_001430"
     CDS             18364..19914
                     /gene="pdeB"
                     /locus_tag="ACHKW0_001430"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HFO7474291.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(19956..20309)
                     /gene="ybaA"
                     /locus_tag="ACHKW0_001431"
     CDS             complement(19956..20309)
                     /gene="ybaA"
                     /locus_tag="ACHKW0_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HFO7474292.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(20429..20525)
                     /gene="ffs"
                     /locus_tag="ACHKW0_001432"
     ncRNA           complement(20429..20525)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="ACHKW0_001432"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            20688..20999
                     /locus_tag="ACHKW0_001433"
     CDS             20688..20999
                     /locus_tag="ACHKW0_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HFO7474293.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            complement(21030..21602)
                     /gene="ybaY"
                     /locus_tag="ACHKW0_001434"
     CDS             complement(21030..21602)
                     /gene="ybaY"
                     /locus_tag="ACHKW0_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HFO7474294.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            21820..22680
                     /gene="tesB"
                     /locus_tag="ACHKW0_001435"
     CDS             21820..22680
                     /gene="tesB"
                     /locus_tag="ACHKW0_001435"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HFO7474295.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            complement(22728..24014)
                     /gene="amtB"
                     /locus_tag="ACHKW0_001436"
     CDS             complement(22728..24014)
                     /gene="amtB"
                     /locus_tag="ACHKW0_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HFO7474296.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(24044..24382)
                     /gene="glnK"
                     /locus_tag="ACHKW0_001437"
     CDS             complement(24044..24382)
                     /gene="glnK"
                     /locus_tag="ACHKW0_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HFO7474297.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(24563..26344)
                     /gene="mdlB"
                     /locus_tag="ACHKW0_001438"
     CDS             complement(24563..26344)
                     /gene="mdlB"
                     /locus_tag="ACHKW0_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7474298.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDR
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(26337..28109)
                     /gene="mdlA"
                     /locus_tag="ACHKW0_001439"
     CDS             complement(26337..28109)
                     /gene="mdlA"
                     /locus_tag="ACHKW0_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HFO7474299.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            complement(28139..28597)
                     /gene="decR"
                     /locus_tag="ACHKW0_001440"
     CDS             complement(28139..28597)
                     /gene="decR"
                     /locus_tag="ACHKW0_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HFO7474300.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(28751..29569)
                     /gene="cof"
                     /locus_tag="ACHKW0_001441"
     CDS             complement(28751..29569)
                     /gene="cof"
                     /locus_tag="ACHKW0_001441"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HFO7474301.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            29669..31369
                     /gene="ybaE"
                     /locus_tag="ACHKW0_001442"
                     /pseudo
     CDS             29669..31369
                     /gene="ybaE"
                     /locus_tag="ACHKW0_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            31434..32129
                     /gene="queC"
                     /locus_tag="ACHKW0_001443"
     CDS             31434..32129
                     /gene="queC"
                     /locus_tag="ACHKW0_001443"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HFO7474302.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(32181..32579)
                     /gene="fadM"
                     /locus_tag="ACHKW0_001444"
     CDS             complement(32181..32579)
                     /gene="fadM"
                     /locus_tag="ACHKW0_001444"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HFO7474303.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(32673..33044)
                     /gene="ybaV"
                     /locus_tag="ACHKW0_001445"
     CDS             complement(32673..33044)
                     /gene="ybaV"
                     /locus_tag="ACHKW0_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HFO7474304.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTTVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(33195..35066)
                     /gene="ppiD"
                     /locus_tag="ACHKW0_001446"
     CDS             complement(33195..35066)
                     /gene="ppiD"
                     /locus_tag="ACHKW0_001446"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7474305.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNEGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(35258..35548)
                     /gene="hupB"
                     /locus_tag="ACHKW0_001447"
     CDS             complement(35258..35548)
                     /gene="hupB"
                     /locus_tag="ACHKW0_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013356995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-beta"
                     /protein_id="HFO7474306.1"
                     /translation="MKRKRRVNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKE
                     GDDVALVGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(35739..38093)
                     /gene="lon"
                     /locus_tag="ACHKW0_001448"
     CDS             complement(35739..38093)
                     /gene="lon"
                     /locus_tag="ACHKW0_001448"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HFO7474307.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(38281..39555)
                     /gene="clpX"
                     /locus_tag="ACHKW0_001449"
     CDS             complement(38281..39555)
                     /gene="clpX"
                     /locus_tag="ACHKW0_001449"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HFO7474308.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(39681..40304)
                     /gene="clpP"
                     /locus_tag="ACHKW0_001450"
     CDS             complement(39681..40304)
                     /gene="clpP"
                     /locus_tag="ACHKW0_001450"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HFO7474309.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(40550..41848)
                     /gene="tig"
                     /locus_tag="ACHKW0_001451"
     CDS             complement(40550..41848)
                     /gene="tig"
                     /locus_tag="ACHKW0_001451"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HFO7474310.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(42192..42509)
                     /gene="bolA"
                     /locus_tag="ACHKW0_001452"
     CDS             complement(42192..42509)
                     /gene="bolA"
                     /locus_tag="ACHKW0_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HFO7474311.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            42814..43392
                     /gene="yajG"
                     /locus_tag="ACHKW0_001453"
     CDS             42814..43392
                     /gene="yajG"
                     /locus_tag="ACHKW0_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7474312.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            43436..44911
                     /gene="ampG"
                     /locus_tag="ACHKW0_001454"
     CDS             43436..44911
                     /gene="ampG"
                     /locus_tag="ACHKW0_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HFO7474313.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT        10630 a        12067 c        11206 g        11129 t
ORIGIN      
        1 gctggttcat acgacgaata aacaggttcg gatcttccag cgtgccgcgt tctgccagca
       61 gcgcctgatc cagcagcagt tctacccact cgctgaactt cgcttcatct tcagtatctg
      121 ccgcacgttt caccagtacg tgatccgggt tcagttcgaa gatgtatttc acttctggca
      181 ctttctggcc cgccgcagcg aacagtttcg ccatctgagt gctcatttcg tccgcgtcgg
      241 tcgaaacgat cgctggcgta tcggtcagac ggtgagtcag acggacatct ttcacgcgct
      301 cgccgagcag ggctttcaca cggtcgatga acggagtcag tgctttctcc gcttctttcg
      361 cgctctcatc aacttcgtca gccagttttt ccagcgactc gtcaacttta gacaccgact
      421 ggaacggttt accgtcgaac tcagtcagat agttcatcat ccactcatcg atgcggtcgg
      481 aaagcagcag aacttcgatg cctttcttac gcagcagttc caggtgcggg ctgctcttcg
      541 ctgccgcata gctgtctgca gtgatgtagt agattttctc ctgcccttct ttcatgcggg
      601 aaacgtagtc ttccagagac acggtctgcg cagaagaatc ggtataagta gaagcaaaac
      661 gcagcagttt ggcgatcgct tcctggttag cgaaatcttc cgccggacct tctttcagta
      721 ccaggccaaa ctgttgccag aaggtctggt atttgtccgc gtcgtctttc gccagttttt
      781 ccagcatttg cagcacacgc ttggtcagcg cattgcgcag gttacgcgtt accgtgctgt
      841 cctggaggat ttcacgggaa acgttcagcg gcagatcgct ggagtcaatc agaccacgca
      901 cgaagcgcag atagttcggc atgaactgtt ctgcgtcgtc catgatgaac acacgctgga
      961 cgtacagttt caggccgtgt ttatgatcac ggttccacat atcccacgga gcctgggacg
     1021 ggatgtacag caggctggtg tactcctgct taccttcaac gcggttgtgg ctccaggtca
     1081 gcggatcatt aaagtcgtgg gcgatgtgtt tgtagaactc tttgtactct tcatcggtga
     1141 tttccgactt gttacgagtc cacagcgcct gcgctttgtt gattttctcc caggagataa
     1201 cggtttcgcc gtctttctct tcgcgttttt cgatctctac cggcagcgcg atatggtcgg
     1261 agtatttgct gatgatggaa cgcacgcgcc agtcatcgag gaactcgtct tcgccttcac
     1321 gcagatgcag ggtgatttca gtaccacgat cttctttggt gatgtcggca acggtatatt
     1381 caccttcgcc agccgattcc cagaagacgc cgttttctgg tttttcgcct gccgcgcgag
     1441 tacgcacggt cactttgtcg gccacgataa acgcagagta gaaaccaaca ccaaactgac
     1501 cgatcagctg gctgtctttc gcctggtcag aacccaggga ttcgaggaat gatttggtac
     1561 cggatttagc gatagtcccc agatggtcaa tcacttcgtc gcgggtcatc cccacgccgt
     1621 tatcggagat ggtcagcgtg cgcttgtctt tatcgaaaga gacacgaacg cgcagttcgc
     1681 catcaccttc gtacaggtcc gggttagata gcgcacggaa acgcagcttg tccgccgcat
     1741 cggaggcgtt agagataagc tcacgcagga agatttcttt attggaatag agagaatgga
     1801 tcatcaggtg cagaagctgt ttcacttctg actgaaaacc acgagtttct tgtcctttca
     1861 tgtaggtcta cctcaataat gccattttaa ggttaaaaac aggatgaggg agagatgggg
     1921 acaagggaga ataatttcaa gcgagagcag gtgatcctgc tctcgtttgc ttaaaaacga
     1981 atcttatgac gcccggcaag ggagtgtgac agcgtggtgc catcgaccat ttccagttcg
     2041 ccgccaaccg gtacgccatg agcgattcgg ctggcttcca cgtcatattg cgcgcaaagc
     2101 tcggcaatgt agttagcggt agcttcacct tcaaccgtgg gattggtggc gaggatcact
     2161 tcagtgattt tttcctctgc cagacgctgt tccagacgat caaggccgat atcatccgga
     2221 ccgatgccgt ccagcggtga caggtgcccc atcaacacaa aataacggcc tgaaaactgc
     2281 cccgtctgct caatggcgta gatgtccgcc ggactctcca ccacacagat ttgaccgttt
     2341 tcctgacgac gcggattcga acagatgtta cagacttcct gttcggtgaa agtgcggcaa
     2401 tcggcgcagt ggccgatttc cgacatcgcc cgggtgagcg cctgcgccag acgcatcccg
     2461 ccgctacgat cgcgctgaag cagcgtgaac gccatacgct gcgccgactt cgggccaacg
     2521 cccggcagac agcgcagtgc ttccataagc tgtgttaaca gcgggctggt ttgcatcaga
     2581 acggcatctt aaagccaggc ggcagctgca ttccggagga tacagaggcc attttttctt
     2641 tctgcgtttc ttcaatacga cgtgctgcgt cgttgaatgc tgcagccacc aggtcttcca
     2701 gcatctcttt gtcgtcttcc agcaggctcg ggtcgatctc tacgcgacgg cagttgtgtg
     2761 caccgttgat ggtcactttt accagacctg cgccagattc gccggtgact tccagctgcg
     2821 cgatctcttc ctgcattttc tgcatttttt cttgcatctg ctgggcttgc ttcatcaggt
     2881 tacccagacc gcctttacca aacataggtt tctctctcaa tcacgttaag gatgacgaac
     2941 gtaagctgtg cttacgatca aatggggcgg atactttctt catccagctc cgcatcgaag
     3001 aaccgacgca gggtctgaat attattatcc gcaataatgg actcgcgcgc ctgcgcaagt
     3061 ttttcttcgt atatcgcctg acgccactcc agcggcgtac gcaccgcggg attatcatct
     3121 tcaacgatag tcagttcaac cgttgaacct tttaacgtgc tcaacgcttc agccagtttt
     3181 tgctgtgcac cgcggttgtt caaatgccgc tgagaggagc gcaaatgcag acatactgcg
     3241 ttgtcgctct cctctttcca ggcatttaag gccacctgtt cgaccagttt tggtagcgaa
     3301 agttgactca cctgtgccgc ccacgcgtcg cgctcaatgg cttctgccgc cagcttcgcc
     3361 gccagttccg gcgttttttc atgttccagc gcttttttca gcgccttcgg cgtggcgacc
     3421 acttcttttt gctgcatcac tggagtggta gccttccagc gatacgcttc ttttttggct
     3481 ggcgcttttt ccagcgccga tggcaccgga cgcgcctgaa cgcgatcggt gaccgaagcc
     3541 agtctttcca gcgcagcgtt attcaccggc cgcgcgcggg tagcggctgc cggttcactc
     3601 ttttttgctt tggttgctcc ctgcacgcgc tgcaactgct ggcgcgccgc cagcacctgg
     3661 ctggtggttt ccgggagcgg tacagtcggt gcctgctgcg gcgctgattg cggttgcggc
     3721 ggcacctggg ttggcgtcat tactgccgtt ggcgcgacgg gcgcaaagga ctggcgtggc
     3781 acttctggct caggcagagg catacgtgga tggaacgcca gcgcgcgcag cagcgtcatc
     3841 tcaacgccca tgcggcggtc cggcgcatac ggtaattctt tgcgaccaat caacagcgtc
     3901 tgatagtaaa gctgaatatc cgtcggcggt atggtgcgcg ccagttcacg catccgcagc
     3961 tcaatggcgg ccatgtcgtt gccaagtgca gcaggcgaaa gttgtaccat cgcaatacga
     4021 tgcaacaggc cgagcatttc caccagcaac gcttcccact cgataccacg ggcagcggct
     4081 tcattaatca gcgccattac gcgctcgccg ttggcctcga ccatcgcttc aaccagcgac
     4141 agcgcctgat cgtcgtcaag cgtacccagc atcgcactga ctgcctgggt tgaaacctgg
     4201 ccgtcaccgc tggcaatcgc ctggtcggtc agacttaagg catctcgcag gctgccttca
     4261 gcggcgcgcg ccagcaattg caacgcccgc ggctcgtgag cgatatgttc ttcgttgagg
     4321 atgtgctcaa gttgatggcg aatttgctcg acatccagcg ccttgagatg aaattgcaaa
     4381 cagcgcgaca aaatcgtcac cggcaatttc tgtggatcgg tcgtcgccag cagaaactta
     4441 acgtgctccg gcggctcttc aagggttttt aacagtgcgt taaagctgtg gcgcgacagc
     4501 atatgcactt cgtcgatcag ataaactttg aaacgaccac gcgccggagc gtactggacg
     4561 ttatccagca ggtcgcgggt atcttcaact ttggtgcgcg aggcggcgtc gatttcaatc
     4621 agatcgacaa agcgcccctg ctcgatttca cgacagttat cgcacacgcc gcacggcgtc
     4681 gcggtaatgc cggtttcgca gtttagcccc ttcgccagca gtcgggcgat agaggttttt
     4741 ccgacgccac gggtaccgga aaaaagataa gcatgatgaa tacgccctaa cgacaagccg
     4801 ttcgccagtg cggtcagcac atgttcctgg ccgacgacgt cagcaaaggt ttgtgggcgc
     4861 cattttcggg ctaagacctg ataactcatt ggcaggctct gaaacgctgg aaggtggatt
     4921 cacgaagggg taatgctaac acagccccgt cagaacggcg aggctgaatg cacatcttgt
     4981 gctgtccgta gcgtgggcag cacaagactg gcgataatta atggcccggg aacgggacaa
     5041 ggctgtagct ggtaatgccc tgtttttcga gacgctgttc gccgccgaga tcgaacaggt
     5101 tgataatgaa cgcagcgtca gccacttcac cacccagacg acggatcagt ttaacggtcg
     5161 cttcgatagt gccgccggtt gccagcagat cgtccaccac cagaactttg tcgcccggtt
     5221 tgatggcatc aacgtggatc tccagctgat cggtgccgta ttccaggtcg taagtttcac
     5281 tgatggtttc acgcggcagt ttgcccggtt tacggaccgg tacaaagcca acgcccagac
     5341 ccagagctac cggagcgcca aacaagaagc cacgcgcttc ggtgccgaca actttggtaa
     5401 tgcccgcatt tttgtaacgc tcaaccagca agtcgatgct gagagcgtaa gctttcgggt
     5461 cttccagtaa gctggtgaca tcgcggaaaa gaatgccggg ttttgggtag tcctgaatgc
     5521 ttttgatgct atttttgaga tactcaagct gctgtgcagt cgcggtcata agtgtatgcc
     5581 tgcttgttac ggtggtactc acggcgcgtt ttaaacgtat caaaaataac agttgtttaa
     5641 cctttgaccg cctgcgcctg tgctcgaaaa cgccagaatg tactggctgt acgcaataat
     5701 tgcaactgcg attgttgtgc ttcagtgctt ttcttgcttt tcatcaatca ccggaattcg
     5761 ccacatatag aaaagcaaac aggcgagaat taccagcaac atgatgcgca cccatggcat
     5821 ctggacgaac cacagagaaa tggcaaacgt gagcaaaata agcaaaatcg cccgcggttt
     5881 gacgccgcgc ggcatcgcat gatgtttctg ccagaaacgt agatagctgc caaaccatga
     5941 gcggtacagc aaccaggcgt gaaagcgcgg ggaagaacgg gcaaagcacc aggccgccag
     6001 caggataaac ggcgtcgtcg gtaataccgg taataccacg cccagcgtac ccagcactac
     6061 cgccagccag ccaatgatga ttaaaatgat tcgttgcata ttgagtgttg tcagcttaca
     6121 gagtggctac tttagcataa caattatcat tttcattgag gtcttatcgt gaaaaccgcc
     6181 ctgctcctgg aaaaactgga aggacagctc gctacgctgc gtcagcgttg tgccccggta
     6241 gcgcaatttg ccacgctaag tgcccgtttt gacagacacc tttttcaaac ccgtgcgaca
     6301 acactgcagg cttgtctcga tgaggcgggc gataatctgg ctgcgcttcg tcatgcagtt
     6361 gagcagcaac agctgccgca agtggcctgg ctggcggaac atctggcggc acaactggaa
     6421 gccatcgcgc gtgaagcctc cgcctggtca ttgcgcgagt gggacagtgc gccaccgaaa
     6481 attgcccgct ggcagcgtaa acgtattcag catcaggatt ttgagcggcg gctacgtgag
     6541 atggttgccg aacgcagagc ccgcctggcg cgggtgaccg atctcgtgga acagcaaacg
     6601 ctgcatcgtg aagtggaagc ctatgaagcg cgcctggcac gctgccgcca tgcgctggaa
     6661 aaaatcgaaa acaggctagc gcgcttaacc cgctagcaat ggagagagta tgtcactgga
     6721 aaatgcccct gacgatgtca aactggctgt cgatttgatt gtgctgctgg aagaaaatca
     6781 gatcccagcc cgcaccgtgc tgcgcgcgct ggatattgta aaacgcgatt atgaaaagaa
     6841 attaacgcgc gatgatgagg cggaaaagtg agaaataaat ggggccgcta aatgtcccct
     6901 aagattgtgg gcaaacgcaa aactgcctga tgcgctacgc ttatcaggcc tacaccgctc
     6961 ctgcaataca ttgaattctt gcggtttcgt aggccggata aggcggtcac gccgcatccg
     7021 gcatgaacaa cgcgcacttt gtcagtaatc tcggggcaac taaatgtcgc cccctttggt
     7081 tattacccta ccgctggcgt cgggtcatcg cctttgtagt cgcgtttgac ttccgtcacc
     7141 tcatcgccct tctcgttgtg cagatgcact tcaagctggt taaaggcaat gttgatgtcg
     7201 ttttcacggc acagctgatc gatagtacgg ttcagctcat cgacagtacg actacggtca
     7261 cgcagttcac gcacatacag acgcagctca tgatccaacg tgctggcacc aaacgccgta
     7321 aagaagactt ccggcattgg ttcgtgcatc acccttgggt gctcagtcgc cgccttcagt
     7381 aacactttac gcactttttc cagatcggag ccataggcca cgccgagacg gatcaccaga
     7441 cgcgtagtag tgtcagtcaa cgaccagttg atcagacgct cggtaacaaa cgctttgttc
     7501 gggatgatca cttctttgcg atcgaaatcg gtaatcgttg tcgcacgaat acggatctta
     7561 cttaccgtcc ccgagaagct accaatggtt accgtatcgc caatacgcac cggacgttcg
     7621 aacagaatga tcaaaccgga gacgaagtta ccgaaaattt cttgtaaacc aaaaccaaga
     7681 cctacggata atgctgcggc cagccactgg agtttatccc aagagacgcc cagcgatccg
     7741 aacaccgtca tcgcaccaac ggcaataatg atgtagttaa ggatggtagt aatggcatac
     7801 gacgcgccct gacgcatatt cagtcgcgag agcaccagca cttccagtaa accaggcagg
     7861 ttgcgaatca acgcccaggc caccattgag gcgataatcg caaacaacag actgcccatg
     7921 gtgacgtttt tcaccaccgc agcgccagct tcagtgccgt tgtaatgcca gagcgtgatg
     7981 ctgtcgagat agctgaacac ggtgatcaaa tcggaccaaa ttgcccagaa catgacaccg
     8041 aacagcgcaa acatcagcaa catggtaata cgcagcgtct gctggttaac ctgttccagt
     8101 gcaatggtgg gttcttccgg cggttcggca ccttctgcgc cctctttcac cagattctgc
     8161 cgacgcgcca gcgcacgacg ccaggcgata cgccgcgccg ctacgcttaa gccgcgcaat
     8221 accgtctggt agagcaagtt ccagatgatc accagataaa cggtttcaat ccaacgcccg
     8281 gagaggcgca gcgtggtgta gaaataaccc gtggcagtca gcaccatcaa tgcaatcggg
     8341 ataatcgaca gcacggtaat ggtgaccagt cgcatggtgt gcgactcttt atcacgccag
     8401 ctttcgcggc acattggcca taccaggaag gcaatcagca gcaggttgaa gaaaatcatc
     8461 gcctgcccca gcacatcatc catcagatgc agcggggaaa gttctgccac cacagaccag
     8521 aaatggatag gcagcaacgc gagactgatg cggacaattt gccgacgcca gtggctggtc
     8581 tgctgctccg gcatgccgaa gtgacgcacg gcaacgccat ttttctccag taccttccag
     8641 cacaggccaa acaccagcca gaaaatcgcc agttttttac tgaacgacca tagcagttcg
     8701 ctgatgttga gctgcatggt caacagaatc aggccaaccg cgagaataat caggcacacc
     8761 ggcagcgcgc ggatcaggtc gataaggatc gcttttggtg tgttgagctg gctatcgtta
     8821 cgcagggaac ccacagcgga agccagtttc tgttgatacg ctttcagcca gcccagacgc
     8881 cagtggatca gcccggcaat caacagcagc ggcaaaccgg cgaggaaagc gataaaaacg
     8941 gcaggccagg ctttttccca gttcaccgtg attttcatcg acttaaattc atctttcagg
     9001 ctttgcggga acgctttgat ccagtcccag tccattggac ggttactgtt cacccaaaag
     9061 atttgctgag tcaggatgga tttcaggttt ttcgatacgc tcattaactg ctgctggttg
     9121 atttgcaggt taatggccat catcagctgg ttacccaact gtttgttgag ttgatccagc
     9181 aattcgcgac gcatatcaac cacttgcaat aacgcatcgt gaacttcgct gttgacttcg
     9241 ttggtgtgac cttcttccag tttgttgacg aacgcatcgc tctggaagag tgcatcacgc
     9301 tgctggttaa cttcaaactg ttcgagacgc aaatccgcga tgcggttggt catgttttcc
     9361 agttcatccg ccgagggcag cgtttgttgt tgctggtaaa ggatacgaga caacagcagg
     9421 ctgcccttca ggacggcaat ctgctcttta atattgcgtt ccgattgcag cgcccgctcc
     9481 agccagtttt tgactttaat gttttgctgc atcaactgat taccgttttc agtcgcggta
     9541 atcagacgct gacttaactg ctggttaatt tccagttcct gtttcaccag cggattagcc
     9601 tgaatacgcg cggcttcatc cggggagacg gcttcctgcg ccgttttttc agttaaagtc
     9661 aggcgcttgc tgtttaccgc ttcttgcaac agctgcaact ggtgctccag acgggcgctg
     9721 ttcgccgtca cgtaatcacg ttgcttttgc aaggtatcct gcaagacggt gttcccttcc
     9781 aggcttttac gctgctggtc aatctccgca ttcagcaacg cctgctgagc ctgcattaac
     9841 actttctggc tgggacgtaa ggcagtctcg ccgacatcag tcccatccag acgactgcga
     9901 atttgttgca gctgctgcga agcgttatac atcgcatttt gcacgcgttc gggctgcgtc
     9961 tgtaacgaaa ccagctggct gttataagac gccagatcgt tttgtgcgtt ttgcaaatcg
    10021 tccagcgcct gggcaacgcg agtttccagc tggcgcaacg aaagcgtact cagaattttg
    10081 cgcgtttctt cgtcgttatc gacatcgcta agtgctgtta acgccgcggt cgcctggcgc
    10141 attttttccg gcgcttcagc gactttttgc cgtagctgaa ctgtctcttc ttttatgcga
    10201 tcgattttat cgagggtggc taatgtatct gtcagatcct gctgcaccag tttgtcctga
    10261 gcagaaagat ctttttgttt atttagtgag tcaagttgcg cctgcaggtc cgcttttgtc
    10321 ggcagatcac cattcgatga cgcccgcgca aacgccgtgt tctggcataa caagacaaaa
    10381 acaaaagcaa taaatgctga aaaaacaaaa tgccgtgatc gtttgtaata ctggaacata
    10441 gtcatgatga atgaaggttt ctgaacctga agaacgacct gaaaaagtca aaccgcaaga
    10501 atatcacgac gcagtgaacc agaatagcaa cgacgaaaat gtccaggaaa aatcctggag
    10561 tcagattcag ggttattcgt tagtggcagg attacgaagc gtggggcaca ggagatacat
    10621 ctccagtaag atggcgacgt aatcgcgggc ttctttttta agatcaaaag attgcggggc
    10681 aaagagccag ttttccatca ggccggaaat atagccgcgc ataataattg ctgcccgacg
    10741 cgtcattaaa tccgcaggca acattttcgc ttcaatacaa tgttttaacg tttgttctat
    10801 acggtcataa ctttccagac agagattacg ttgtgcctgt tgcacaacag ccatttctcc
    10861 gacaaattcg catttgtgga atataatctc catcaataat cgacgccgtt cttctgtcac
    10921 cgtggattca agaacatgaa ttaatatctc tcttaatact gagagtggat cgccagggaa
    10981 ttttgcctga tactcaagct ctagttcacc aatattggat tctgacagtt cccagatctc
    11041 actgaacaaa tccgacttgt ctttaaaatg ccagtagatt gcaccgcgcg taacgccagc
    11101 tgcttttgca atctcgccca gcgaggtgga tgataccccc tgctgtgaga aaagacgtag
    11161 agccacatcg aggatgtgtt ggcgcgtttc ttgcgcttct tgtttggttt ttcgtgccat
    11221 atgttcgtga atttacaggc gttagattta catacatttg tgaatgtatg taccatagca
    11281 cgacgataat ataaacgcag caatgggttt attaactttt gaccattgac caatttgaaa
    11341 tcggacactc gaggtttaca tatgaacaaa aacagagggt ttacgcctct ggcggtcgtt
    11401 ctgatgctct caggcagctt agccctaaca ggatgtgacg acaaacaggc ccaacaaggt
    11461 ggccagcaga tgcccgccgt tggcgtagta acagtcaaaa ctgaacctct gcagatcaca
    11521 accgagcttc cgggtcgcac cagtgcctac cggatcgcag aagttcgtcc tcaagttagc
    11581 gggattatcc tgaagcgtaa tttcaaagaa ggtagcgaca tcgaagctgg tgtctctctc
    11641 tatcagattg atcctgcgac ctatcaggcg acatacgaca gtgcgaaagg tgatctggcg
    11701 aaagcccagg ctgcagccaa tatcgcgcaa ttgacggtga atcgttatca gaaactgctc
    11761 ggtactcagt acatcagtaa gcaagagtac gatcaggctc tggctgatgc gcaacaggcg
    11821 aatgctgcgg taactgcggc gaaagctgcc gttgaaactg cgcggatcaa tctggcttac
    11881 accaaagtca cctctccgat tagcggtcgc attggtaagt cgaacgtgac ggaaggcgca
    11941 ttggtacaga acggtcaggc gactgcgctg gcaaccgtgc agcaacttga tccgatctac
    12001 gttgatgtga cccagtccag caacgacttc ctgcgcctga aacaggaact ggcgaatggc
    12061 acgctgaaac aagagaacgg caaagccaaa gtgtcgctga tcaccagtga cggcattaag
    12121 ttcccgcagg acggtacgct ggaattctct gacgttaccg ttgatcagac cactgggtct
    12181 atcaccctac gcgctatctt cccgaacccg gatcacactc tgctgccggg tatgttcgtg
    12241 cgcgcacgtc tggaagaagg gcttaatcca aacgctattt tagtcccgca acagggcgta
    12301 acccgtacgc cgcgtggtga tgccaccgta ctggtagttg gcgcggatga caaagtggaa
    12361 acccgtccga tcgttgcaag ccaggctatt ggcgataagt ggctggtgac agaaggtctg
    12421 aaagcaggcg atcgcgtagt aataagtggg ctgcagaaag cgcgtcctgg tgtccaggta
    12481 aaagcacaag aagttaccgc tgataataac cagcaagccg caagcggtgc tcagcctgaa
    12541 cagtccaagt cttaacttaa acaggagccg ttaagacatg cctaatttct ttatcgatcg
    12601 cccgattttt gcgtgggtga tcgccattat catcatgttg gcaggggggc tggcgatcct
    12661 caaactgccg gtggcgcaat atcctacgat tgcaccgccg gcagtaacga tctccgcctc
    12721 ctaccccggc gctgatgcga aaacagtgca ggacacggtg acacaggtta tcgaacagaa
    12781 tatgaacggt atcgataacc tgatgtacat gtcctctaac agtgactcca cgggtaccgt
    12841 gcagatcacc ctgacctttg agtctggtac tgatgcggac atcgcgcagg ttcaggtgca
    12901 gaacaaactg cagctggcga tgccgttgct gccgcaagaa gttcagcagc aaggggtgag
    12961 cgttgagaaa tcatccagca gcttcctgat ggttgtcggc gttatcaaca ccgatggcac
    13021 catgacgcag gaggatatct ctgactacgt agcggcgaat atgaaagatg ccatcagccg
    13081 tacgtcgggc gtgggtgatg ttcagttgtt cggttcacag tacgcgatgc gtatctggat
    13141 gaacccgaat gagctgaaca aattccagct aacgccggtt gatgtcatta ccgccatcaa
    13201 agcgcagaac gcccaggttg cggcgggtca gctcggtggt acgccgccgg tgaaaggcca
    13261 acagcttaac gcctctatta ttgctcagac gcgtctgacc tctactgaag agttcggcaa
    13321 aatcctgctg aaagtgaatc aagatggttc ccgcgtactg ctgcgtgatg tggcgaagat
    13381 tgagctgggt ggtgagaact acgacatcat cgcagagttt aacggccaac cggcttccgg
    13441 tctggggatc aagctggcga ccggtgcaaa cgcgctggat accgctgcgg caatccgtgc
    13501 tgaactggcg aagatggaac cgttcttccc gtcgggtctg aaaattgttt acccgtacga
    13561 caccacgccg ttcgtgaaaa tctctattca cgaagtggtt aaaacgctgg tcgaagcgat
    13621 catcctcgtg ttcctggtta tgtatctgtt cctgcagaac ttccgcgcga cgttgattcc
    13681 gaccattgcc gtaccggtgg tattgctcgg gacctttgcc gtccttgccg cctttggctt
    13741 ctcgataaac acgctaacaa tgttcgggat ggtgctcgct atcggcttgt tggtggatga
    13801 cgctatcgtt gtggtagaaa acgtggagcg tgttatggcg gaagaaggtt tgccgccaaa
    13861 agaagctacc cgtaagtcga tggggcagat tcagggcgct ctggtcggta tcgcgatggt
    13921 actgtcggcg gtattcgtac cgatggcctt ctttggcggt tctactggtg ctatctatcg
    13981 tcagttctct attaccattg tttcagcaat ggcgctgtcg gtactggtag cgttgatcct
    14041 gactccggct ctttgtgcca ccatgctgaa accgattgcc aaaggcgatc acggggaagg
    14101 taaaaaaggc ttcttcggct ggtttaaccg catgttcgag aagagcacgc accactacac
    14161 cgacagcgta ggcggtattc tgcgcagtac ggggcgttac ctggtgctgt atctgatcat
    14221 cgtggtcggc atggcctatc tgttcgtgcg tctgccaagc tccttcttgc cagatgagga
    14281 ccagggcgta tttatgacca tggttcagct gccagcaggt gcaacgcagg aacgtacgca
    14341 gaaagtgctc aatgaggtaa cgcattacta tctgaccaaa gaaaagaaca acgttgagtc
    14401 ggtgttcgcc gttaacggct tcggctttgc gggacgtggt cagaataccg gtattgcgtt
    14461 cgtttccttg aaggactggg ccgatcgtcc gggcgaagaa aacaaagttg aagcgattac
    14521 catgcgtgca acacgcgctt tctcgcaaat caaagatgcg atggttttcg cctttaacct
    14581 gcccgcaatc gtggaactgg gtactgcaac cggctttgac tttgagctga ttgaccaggc
    14641 tggccttggt cacgaaaaac tgactcaggc gcgtaaccag ctgcttgcag aagcagcgaa
    14701 gcaccctgat atgctgacca gcgtacgtcc aaacggtctg gaagataccc cgcagtttaa
    14761 gattgatatc gaccaggaaa aagcgcaggc gctgggtgtt tctatcaacg acattaacac
    14821 cactctgggc gctgcatggg gcggtagcta tgtgaacgac tttatcgacc gcggtcgtgt
    14881 gaagaaagtt tacgtcatgt cagaagcgaa ataccgtatg ctgccggatg atatcggcga
    14941 ctggtatgtt cgtgctgctg atggtcagat ggtgccgttc tcggcattct cctcttctcg
    15001 ttgggagtac ggttcgccgc gtctggaacg ttacaatggc ctgccatcca tggaaatctt
    15061 aggccaggcg gcaccgggta aaagtaccgg tgaagcaatg gagctaatgg aacaactggc
    15121 gagcaaactg cctaccggtg ttggctatga ctggacgggg atgtcctatc aggaacgcct
    15181 ctccggcaac caggcacctt cactgtacgc gatttcgttg attgtcgtat tcctgtgtct
    15241 ggcggcgctg tacgagagct ggtcgattcc gttctccgtt atgctggtcg ttccgctggg
    15301 ggttatcggt gcgttgctgg ctgccacctt ccgtggcctg accaatgacg tttacttcca
    15361 ggtaggcctg ctcacaacca ttgggttgtc ggcgaagaac gcgatactta tcgtcgaatt
    15421 cgccaaagac ttgatggata aagaaggtaa aggtctgatt gaagcgacgc ttgatgcagt
    15481 gcggatgcgt ttacgtccga tcctgatgac ctcgctggcg tttatcctcg gcgttatgcc
    15541 gctggttatc agtactggtg ctggttccgg cgcgcagaac gcagtaggta ccggtgtaat
    15601 gggcgggatg gtgaccgcaa cggtactggc aatcttcttc gttccggtat tctttgttgt
    15661 ggttcgccgc cgctttagcc gcaagaatga agatatcgag cacagccata ctgtcgatca
    15721 tcattgatac aacgtgtaat cactaaggcc gcgcaagcgg ccttttttat gcataaccta
    15781 cgaacattaa ggagtaattg aaccaccaac tcaggatctc atacgaaaac cagtattaac
    15841 cacggataaa attcataaaa aatactgatt gttagttaat ttatattaag tagcgctaat
    15901 agatttaata atccataatc atttagaggc tattcttaat tatttgcggt aattctttat
    15961 tcattcctcg gttattacgt catattcaga gcaatcctgg tattagtgtc accaatttca
    16021 tctggcgata atcctgaaat gttatgaaca gttcgagcaa actgctttta cctgctgcgg
    16081 gttagtgcta gtatgaaaaa gtgagtcctg tcccgattcc ttcctaattg taatttttcg
    16141 taataatgcg atgaaaacct gcaaagagtg gcttatagtt aagctaacaa acgagagggc
    16201 aagtccaggt cagtaagttt tttccatccc gaaaggtgtc cgttagttca accactaaga
    16261 aggggacgcg ttatggatga atactcaccc aaaagacatg atatcgcaca gcttaagttt
    16321 ctctgtgaaa ccctgtatca tgactgtctt gcaaaccttg aagaaagcaa tcatggctgg
    16381 gttaacgacc caacctcggc gatcaacctc cagttgaatg aactgattga gcatattgcg
    16441 accttcgcac ttaattacaa aattaagtat aatgaagaca ataagctcat tgagcagatc
    16501 gacgaatatc tggatgacac ctttatgttg ttcagcagtt atggtattaa tatgcaggat
    16561 cttcagaaat ggcggaagtc aggtaatcga ctattccgtt gttttgtcaa tgcgacgaaa
    16621 gagaatcctg cgagtttatc ttgttagaat tattacaacc atgggtagaa gtatgtccga
    16681 aaaaccttta acgaaaaccg attatttaat gcgtttacgt cgttgccaga caattgacac
    16741 gctggagcgt gttatcgaga aaaataaata cgaattatca gataatgaac tggcggtatt
    16801 ttactcagcc gcagatcacc gcctcgccga attgaccatg aataaactat acgacaagat
    16861 cccttcctca gtatggaaat ttattcgcta ataaataatt cgctttcgga gctataaccg
    16921 gctgtttatt aagaatttta tactttttca ccatgaagac ataccctatg tgatctttat
    16981 cacacagatg taatgggaac gttctcttca ctgacttttc gtcttactgt gttgccgcat
    17041 tttcagcaac cggaggtcag taatgagcac agaaaaagaa aagatgattg ctggtgagtt
    17101 gtatcgctcg gcagatgaga cgttatctcg cgatcgcctg cgcgctcgtc agcttattca
    17161 ccgatacaat cattccctgg cggaagagca cacattacgc cagcaaattc tcgctgatct
    17221 attcggtcag gtgacagagg cttatattga gccaacgttt cgctgtgact atggctataa
    17281 catttttctc ggtaataatt ttttcgccaa cttcgattgc gtgatgcttg atgtctgccc
    17341 tattcgcatc ggtgataact gtatgttggc accaggcgtt catatctaca cggcaacaca
    17401 tcccatcgac cctgtagcac gtaatagcgg tgctgaactg gggaaacccg tcaccatcgg
    17461 taataacgtc tggattggcg gacgcgcggt cattaaccct ggtgtgacca ttggtgataa
    17521 cgtcgtggta gcctcaggtg cagttgtcac aaaagatgtc ccggacaacg ttgtcgtggg
    17581 cggtaatcca gccagaataa ttaaaaaatt gtaatcggtt tttcgcaact gttatgcaaa
    17641 attgtggtaa atctgttact tcccctctac tattcccacg ttaaaatagg gtgttccctg
    17701 gaaagttgca gataccacga aggcaaacga tgaccgaaat acaacgcctg ctgaccgaaa
    17761 cgattgagtc tctgaatacc cgcgaaaaac gcgacaacaa accccgcttt agtatcagtt
    17821 ttatccgtaa acatccgggg ctgtttatcg gtatgtacgt tgcttttttt gccaccctgg
    17881 cggtgatgtt gcagtccgaa acgctgtcag gctctgtctg gctactggtt gtattattta
    17941 tcctgcttaa tggtttcttc tttttcgatg tctacccacg ctaccgctat gaagatatcg
    18001 acgtgctgga tttccgcgtt tgctataacg gcgaatggta caacacgcgc tttgtacctg
    18061 ccgcgctggt tgaagccatc ttgaactctc cgcgtgtcgc ggatgttcat aaggaacaac
    18121 tgcaaaaaat gatcgtccgt aaaggtgaac tgtcttttta cgatattttt accctcgctc
    18181 gcgccgaatc aacatcttaa gttagggtta cataccaggc gtaaagctct gcgcctggtc
    18241 aaatgacaat gatcgtttcc acccatcact tcatgaaata ccagctctac ctccttatct
    18301 cctgccagcc tttttccaca atcagatata ctttccctac actgtgttaa taaggatatg
    18361 ctggtgagaa cacgacatct ggtcggcctt atttcgggag tactgattct ttcagtattg
    18421 ctgcctgtcg gcttaagcat ctggctggcc catcagcagg tagaaacatc gtttattgaa
    18481 gagctggata cctattcctc ccgcgtcgct attcgggcca ataaggtggc gacacaaggg
    18541 aaagatgcgc tgcaagagct ggaaagatgg caaggcgctg cctgtagcga agcccatctc
    18601 atggaaatgc gtcgggtatc ttacagttat cgctatattc aggaagtggt ttatatcgat
    18661 aacaacgttc cccagtgttc gtctctggag catgaaagtc cgcccgatac cttccccgag
    18721 ccaggtaaaa tttcgaaaga tggttatcgt gtctggttaa catcgcataa cgatttaggc
    18781 attatccgtt acatggtcgc catgggaacg gcacattatg tcgtcatgat cgaccccgct
    18841 tcctttattg atgtcattcc ctatagctca tggcaaattg atgccgccat tattggcaat
    18901 gcccataacg ttgtcataac cagcagcgat gaaattgctc agggaattat taccaggcta
    18961 caaaaaacac ccggtgagca tatcgaaaat aatggaatca tttacgatat cctgcccttc
    19021 ccggagatga atatttcgat catcacctgg gcttcaacga aaatgttgca gaaaggctgg
    19081 catcggcaag tctttatttg gttaccgctc gggttggtga ttggcctgct ggcagcgatg
    19141 tttgtgctgc gtattttgcg ccgtatccag tcaccgcatc atcggctgca ggatgctatc
    19201 gaaaatcgtg atatttgcgt gcactatcag ccgattgtct ccttagccaa tggcaaaatt
    19261 gtcggtgctg aggcactggc gcgctggccg cagacagacg gtagttggtt gtcaccagat
    19321 agttttattc cgctggcaca gcaaacgggc ctttctgagc cattgacgct actgattata
    19381 agaagcgtct ttgaagatat gggcgactgg ctgcgtcagc atccacagca gcatatttcg
    19441 atcaatcttg aatccaccgt gctcacctcg gaaaaaatcc cgcaattgct gcgtgaaatg
    19501 atcaatcact atcaggttaa tcccagacag atcgcgcttg aactcactga acgcgagttt
    19561 gccgatccga aaaccagcgc cccgataatt tctcgctacc gggaggcggg ccatgaaatt
    19621 tatcttgatg attttggtac gggatattca agtttaagtt atttacagga tctggatgtc
    19681 gacattctga agatcgataa atctttcgtt gatgcgctgg aatataaaaa tgtcacgccg
    19741 catatcatcg aaatggcaaa aacactgaaa ctgaaaatgg tagcggaggg aatcgaaacc
    19801 agtaaacaag aagagtggct acgccagcat ggcgtgcact acggtcaggg ctggctctac
    19861 agcaaggcat taccgaaaga agatttctta cgctgggccg agcaacattt gtgaattttt
    19921 gggctgacgg cacgccgcac agcccgtcac gctacctatt cgtcgatgat tgactcgaat
    19981 ccgccataga tcattcgctt gccgtcaaac ggcatggact cgccgaactc tttcatccgt
    20041 gggtccgaca tcatcttttg agtagcagcg tcgcggacct cttttgaagg gtattcaatc
    20101 cagctaaaga caacctcttc attctcttcc gctttcaccg ccatacgaaa atcggtcact
    20161 ttgccatccg gtacatcgct ggcccagcat tcgacaatac gaagcgcgcc aaactcttta
    20221 aacaatggcg cagccttagc ggccatttcc cgataggcat cctttttatc ggcaggaacg
    20281 gcaaccacaa aaccatcaac atacttcatc agacatacct ccgaagcgta ctgcgcagcc
    20341 agacaattct tgcgacgtca gttgtggtta aagtttagcc atgttggggt tttttgacga
    20401 aacggtggga ggcagaaatg ggtgggggcc ctgccagcta catcccggca cacgcgtcat
    20461 ctgccttggc tgcttccttc cggacctgac ctggtaaaca gagtagcgtt gcgggagaac
    20521 caacagagcc cccattgaga gcgttgagaa ccaacgcgca ggcgcattat cactgctgcc
    20581 ccggctaatt gcaacccatc gcgtgccaga tgctggtttc ttgcgcaatg cgacttcact
    20641 cgggcgtttt tccagattat gctgaaaaat tacctcagga agaaaagatg gaaaaagaag
    20701 attcatttcc ccaacgcgtc tggcaaatcg tcgccgctat tcccgaaggc tatgtcacca
    20761 cttacggtga tgtggcgaaa ctggcgggat cgccccgcgc cgcgcgccag gtgggcggtg
    20821 tgttaaagcg tctccctgaa ggcagcacct taccctggca ccgggtggtt aatcgccacg
    20881 gcacaatttc gctaaccgga ccggatttac agcgtcagcg acaggcatta ctggcagaag
    20941 gtgtgatggt atcgggaagc gggcaaatcg acttgcagcg ttatcgctgg aactactaac
    21001 cctctccggg cggagagggt taacgtcgct taatattgcg taggagctgg aactgccgaa
    21061 gacggattca cctgagttgg tgaagtcgaa ggtacggtag tcgttgcacc accgctggcc
    21121 tgaaccggca cggcggtttg ctgcaccggc accaatgtca ggtcggcttt agttccgccc
    21181 ttgttgatca ccggctgaac ggtatcggta ataaatacca gtttgtcatt cacggtaatc
    21241 gccgcactca acagaatacg cgcgttcggc tgaacatctg ccgggttaaa tggcagaaca
    21301 aagctgaatg gtgactgttt accttcagta cgcaccgctt tctgcgccag cacttttgac
    21361 ggtgcatcgg ctaacgacgc gtcagaaagt gtcacggtca gcacagcatc aggcggcagt
    21421 gcgactttct gacggatcca gacggtaccg gagacattcg gttgctggat agcaggttgt
    21481 tgtgttgctg aaatagacgt atttgcagcc ggggctggcg tctgaatatc cgcgctttta
    21541 tctgcgcaag ccgccaacgc aatcgcaacc gctaaaccac tggccatgtg cacgagtttc
    21601 attcatttct ccttattatc aatgcaccag cgggctaact ttcctcgccg gaagagtggt
    21661 taacaaaata gtaacgtcaa caagtgtggc acacatcacg catttctgcc tgtaattagc
    21721 ccgtaattca gaccattgca cccatcggac cagtagcaaa tttgcgttat actcaactca
    21781 ctttggcttg ctgcggcagc tttgttactg gagagttata tgagtcaggc gctaaaaaat
    21841 ttactgacat tgttaaatct ggaaaaaatt gaggaaggac tctttcgcgg ccagagtgaa
    21901 gatttaggtt tacgccaggt gtttggcggc caggtcgtgg gtcaggcctt gtatgctgca
    21961 aaagagaccg tccctgaaga gcggctggta cattcgtttc acagctactt tcttcgccct
    22021 ggcgatagta agaagccgat tatttatgat gtcgaaacgc tgcgtgacgg taacagcttc
    22081 agcgcccgcc gggttgctgc tattcaaaac ggcaaaccga ttttttatat gacggcctct
    22141 ttccaggcac cagaagcggg tttcgaacat caaaaaacaa tgccgtccgc gccagcgcct
    22201 gatggcctgc cttcggaaac gcaaatcgcc caatcgctgg cgcacctgct gccgccagtg
    22261 cttaaagata aattcatctg cgatcgtccg ctggaagtcc gtccggtgga gtttcataac
    22321 ccactgaaag gtcacgtcgc agaaccacat cgtcaggtgt ggatccgcgc aaatggtagc
    22381 gtgccggatg acctgcgcgt tcatcagtat ctgctcggtt acgcttctga tcttaacttc
    22441 ctgccggtag ctctacagcc gcacggcatc ggttttctcg aaccggggat tcagattgcc
    22501 accattgacc attccatgtg gttccatcgc ccgtttaatt tgaatgaatg gctgctgtat
    22561 agcgtggaga gcacctcggc gtccagcgca cgcggctttg tgcgcggtga gttttatacc
    22621 caggacggcg tgctggttgc ctcgaccgtt caggaagggg tgatgcgtaa tcacaattaa
    22681 aaaaatagcc ggaggtaaaa ccgtccggct attttttgca gtgcttgtta cgcgttatag
    22741 gcattctcgc cgtgactgtt gacatccagc ccttctcgct cctgctcttc cggtacacgc
    22801 agaccaaccg tcagatccgc cagtttgtag ccaataaatg ccaccacacc ggaccagacg
    22861 atcgtaatgg cgatgctttc cagctgtacc agcaactgat ggcccatcgt cacaccttca
    22921 gcgaagccca cgccacccag cgaactggcg gcaaaaatcc cagtcatgat acagccgaca
    22981 atgccacaaa cgccgtgcac accgaagaca tcgcagggat catccacccg cagcaagcgt
    23041 ttgagcatag taacgcccca caagcccgcc agaccagcta ccacgccgat aatcaacgcg
    23101 ccgccaaccc caatgtagcc acacgctggc gtcacgccga ccagaccggc aatcgcgcca
    23161 gaacacgctc ccagcagaga aggcttacca cgcagcgccc attcaccgaa gatccagcca
    23221 agaattgccg ccgccgttgc gaccacagta ttcacaaatg ccagtgccgc gatttcattc
    23281 gccgtgcccg ctgacccggc gttaaagcca aaccaaccaa tatagagaat ggcagtaccg
    23341 gtgaagacca ttggcaggtt gtgcggttta aacgcctctt tgccgaagcc cacgcgtttg
    23401 cctatcagat acgcgcccac cagaccggcg attgcggcgt taatatgcac caccgtgcca
    23461 cccgcgaaat ccagcgcacc gtgagaagcc agcaaaccac cgccccacac catatgcgca
    23521 atcggaatgt aagagagcgt cagccatacc accacgaaaa tcaacacagc tgagaagcgg
    23581 attcgttccg ccagcgcccc aacgatcaag ccgacggtaa tgcaggcaaa cgagccctga
    23641 aacgccacgt ggatatactg ataaatgctg cccatcaccg ccgtcagttc gatgtttttt
    23701 agcatcaacc agttaatgtt gccgaagaag ttgttgccct caccaaacgc cagcgagtaa
    23761 ccgtaaacca cccagagaat acagaccagt gcaaacgtca ccgtcacctg cgtcagcatc
    23821 gacagcacgt ttttgccgcg aatcaaccca ccgtaaaaca gggcaatccc cggaatagtc
    23881 ataaacagca ccagcgcagt acaaatcatc ataaacgcat tgtcggcttt atcggccacc
    23941 gcaggtgcag ccattaccag tcccggcagc atcgccagtg aagcaagccc agtttttatc
    24001 gtcgctatct tcatttttcg ttcctgttgc tgtgtgccag agattacagc gccgcttcgt
    24061 cggcttcgcc ggtacgaata cgaatgacgc gttgcaattc agcgacgaag attttgccgt
    24121 cgccaatttt tccggtgtaa gccgccttac tgacgatatc gatcacttca tcgagttggt
    24181 catcagcaat cgccacatca atttttactt ttggcaggaa gttgacgctg tattccgccc
    24241 cccggtacag ctcggcatgc cctttctgac gcccgaaacc tttcacttcg gtgacggtca
    24301 ggccctgaat accaatggaa gataacgctt cacgaacgtc ttccagcttg aatggtttga
    24361 ttatcacggt caccagcttc atagatcccc tccggtcaga attcggtaat ggttctgcta
    24421 cacgaagaag gtattgcaag cggtgtgcca gaaatgaaaa acgagaaaga gagcaggaag
    24481 ttaagaagat tgaaaatgaa aacgcactat gacagtgcac agtgcgttac atttttgcac
    24541 caacgatgaa atattgcgct attcaggcgc tcaatgattc ttcttcacgc acgctggctg
    24601 ccagctcttc gcccgcaagt tgcagttgat acatctgcca gtagcgtccc tgggccgcca
    24661 gcagttgctg gtgagtgccc tgctccacgg cttgcccacg atgaagcacc agaatggtgt
    24721 cggcatcaac aatggtcgat aagcggtgag caatcaccac cagcgtggta tgttcacgca
    24781 cagccgccag agcatgttga atcgcctgtt cagtaccgga gtcaatgctg gcggttgcct
    24841 catcaaggat caggatttgc ggcgtctcca ccaacacgcg tgccagtgcc agcagttgct
    24901 tttgcccgac ggagagatta ttcccctgct cgcccagcgg cgtataaata ccatcactca
    24961 tgctgcgcgc cagctccgcc agttgcacgg tttccagcgc ctgccagacg cgttcttcgg
    25021 agatatcacg ccccagcgtc acgttggcga ggaaggtatc cgccagcacc accggatctt
    25081 gctgcaccat tgccacgccc tggcgcagcg cgctgtgact tagcgaactt aatggacgac
    25141 catcaaggcg aatctcacct tccgttagcg ggtaatagcc catcaataaa ctggcgaggg
    25201 tgcttttgcc actgccggta tgcccgacca gtgccacaaa gttacgcgaa ggcacagaga
    25261 gattaatgtt ctttagcacc agattgtcat cgcgataagc aaatgacacg ttatcgactt
    25321 cgatggtgcc actctgtaac gggcgatcat cattgccata ttgctggcgc ggtccgtcca
    25381 tcagttcaaa cacgcgctca ccagcaacaa cagcctgttg cagcatcgcc tgttgcgtgg
    25441 tcagttcgat taatggttcg ttaagtcgcc caagatagct gataaacgca tacagcacgc
    25501 ccacttcaat ggtgccgcta gcggagaagc caaacagcat caacaagcca caaagaatga
    25561 gcgatgaaaa cagactcagc agcggacgca gcagaaaacc gtcgaggcgc agggtttgca
    25621 tcctggccat atagtgtgaa cgactggcct cccccatacg ttcgccaaat cgcgcctgct
    25681 gacggaactg ctggataacg ctcatgccat tgatgatttc gttaaagccg tcgttgatat
    25741 ccgctagata ggcgcgcaca cgacggacaa tcggcgtgct gtaacgctgg tatatcacca
    25801 ttaccaccag caccaccggg aaaatcatta tcgccaccag cgccattcgc cagtcgaggc
    25861 tgaacatcgc caccagcatc gcgcccacca gtgcggcgct acgcaggaca gttgccacta
    25921 cggtaacgta gagatcgcgg atcacttcag tgtcattagt gacgcgggaa atcacctgcc
    25981 cgacgggttg ggtatcaaac tcgcttaatg gctggcgcaa tgcggcatcc atcacgtcgg
    26041 tacgcaactg ttgcactacg cctactgccg cccgattaaa cagcagcgac tgcgcgtaat
    26101 gtagcccggc ggcaaacagt tgtagcccaa catacgccgc agccagccct gcaaccattt
    26161 tcaacggcag gttatttttc gctaccatat tgtcgataaa atagctgata agcagcggcc
    26221 cactgacttc tgccgctgcc gcaacccaca tcatcaggac cgcaatcccc agcggtttac
    26281 gccacggcga accgtacgct aacagacgct tgagagtcgg ccacagttgg ctaaaactac
    26341 gcatcgacgg cctcctcgcg aatttccgga gcgtcgtcga gcgccgcctc cagttgttga
    26401 tagcgataca tatcgcgata ccagccgctt tgttgcgcca gctcatcatg attgccacgc
    26461 tgggcgatat gtccgtgctg catcacaata atttcactgg cttccgtcag tgccgaaagg
    26521 cgatgggcac tgatgattac cgttcttccc tgtccccact gacgcaggtt atgcaggatc
    26581 tggtgctctg tgcgtccgtc caccgccgaa agcgcatcat caaggatgag gatttccgcg
    26641 ttgactaata acgctcgagc aatggagata cgctgttttt gcccaccgga aagcatcacg
    26701 ccgcgctcgc ccacttctgt atcgtaacct tgcggtagac gcaaaatatc gtcatgtacg
    26761 ctggctaacc gcgcgacatg ctcaatctct tgctgggtgg cattcgggca acccaacgcg
    26821 atgttgttcg ctacggtgtc ggaaaaaagg aacggtgtct ggctaactac cgccaggcgg
    26881 ctacgccagc tatcgagttg taacttcgtc agaggaatat catgaaagcg aatatccccc
    26941 tcgctgacgt cgaaatgacg ctgaatgagc gacaacaggg tacttttgcc ggaaccagtc
    27001 ggcccgcaga tacccagcat ctgaccgggt ttcagggcga aattgacgtt ttccagcgca
    27061 ggatggtcag tctgcggata cgtgaactgg tgaatattta catcaagttc gccacgccct
    27121 tccggcacgg gttcgctacc atcgttcacc accggcgctt ccgccagcat cgcacgaata
    27181 cggctgtacg cagcactacc acgttccaca atgttaaaca tccatgccag cgccagcatt
    27241 ggccaaatca tcagacctaa atacatcata aaactggtga gctggcccag cgttaaactg
    27301 ccctgcgcca ccatccaact accgccgcca atcgccagca agttcgccat accaatcgcg
    27361 atatagatgg ttgggtcgaa acgagcatca atacgcgcca cccgcatgtt ttttttgccg
    27421 gtatcttcgg catccgcggc aaataacgcc gactggcgat cttccagacc aaaggctttg
    27481 atcatgcgga tactggtgag gctttcctgg gtgcggtcat taagactaga aaacgccgcc
    27541 tgtgccagct taaagcgttc atgcaaggca tcgccgttgc gcttgatcat gatcgccatc
    27601 actggcatcg gcaacagggc aaataaggtc aactgccagc taatttgcgt agacatcata
    27661 atcaacacag cgcagcccat caccagtgaa tccaccagcg tcagcacccc ttctccggcg
    27721 gcaaacacga cgcgatcgac gtcattggtc gcacgagcca tgagatcacc cgtgcgatga
    27781 cgcaggtaaa actcaggatg ctgccggctt agctgacggt aataatcttc acgcagttca
    27841 acagccagtt gataagacgc accaaacagc aatacccgcc agacgtaacg caggagataa
    27901 accacaacgg caatcagcac catggtggcg atccacatca ggatctgccc ggtagtaaag
    27961 tgttgttctg tcacgccatc gacaacaata ccaaccactt ttggcggaac cagttgcagc
    28021 atcgcgataa tgacaagcaa ggcgacagcc ccgagatagc gacgccattc ccgacggaaa
    28081 taccagctta attgagcaaa taatcgcacg cgttgtgtcc tgacctgatt ctggatattt
    28141 attcgatggg taaagaagtg gtgtatttaa tctgttccat cgcgaagctg gaagtgacgt
    28201 ccgacagccc cggcacgctg tttaccagac gcttataaaa ctcgtcgtag cgtttcatgt
    28261 cggcaacctg gacgcgcatc agataatcgt attcaccagc catgcgccag aaccccagca
    28321 cttctggcat ttcggtaacc accgtgacaa agcggcaata ccattcgctg ctgtgatgtt
    28381 gcgtttttat cagcacaaag gcggtcaggc cgaggcctat tttttccgga tccagcaggg
    28441 cgactttgcc gataaggata ccgtcgtcct ccagccgttt caggcgcttc cagcaagggg
    28501 tggttgtcag attaacggct tcagccagtg cctgcaaaga gagggtgcaa tcctgctgca
    28561 gtaaggccag cagcttacgg tcaattttat ctaacatagc ccttccacgg agaatttttt
    28621 tctcttttaa ttctatttta aaggtgaaat cgcgacaatt tattctgtgc ggtttaaaca
    28681 ccacggcaca aaatgacaaa ttcaacgcaa aaaaaaaccg ggcaattgcc cggtgctgga
    28741 agggatctcg ttattcgggg gaataaggta gatgtggata gtccagccag tgcgtcaaat
    28801 agtgagagac agcctgattt cggcaatgtc caatcaccgg taaatgcggg agctccgcgc
    28861 gcagttgcgg catcgcattg cccataataa acccgctacc gacgctgcct aacatttcgc
    28921 gatcgttcat cgcatcacca aaggccatgc aatcgcgcaa cgataaacct aaatgttggg
    28981 tcagcaccgt caatgcagcg cctttattgc agcccaccgg cagcacttcg aggcaatccg
    29041 tggcggaaaa acacaaatgt gcacgctcgc ctaatgcttc gtatagctgg atctgcaagc
    29101 gtgtaagatc gtcgtgatcg ccacagaagc agatcttggt gacgctgccg agtggcattt
    29161 ttttgacatc gattatctga taacgaaaac cgctatagac aaatgcctgc aacaacgcag
    29221 ggatctcttt cccggtaaac caaccgtcgt cattgaagat atgcatgctg gctcgggtat
    29281 cccattgctg atacagcacc agctccgcga catccgcagg taaatcatca cgatgtaaaa
    29341 gttcaccttc cagagaatgc acgcgcgttc cgttgccggt aatcaaatat gcatccagcg
    29401 atagcgcccc gagaatatgc tgcatctcca gcgcatgacg ccccgtggca aaagtgaggg
    29461 taatgtcgcg ttcacgcagt cgcgccaaag tagagagggt tttctcacct aaatgatggt
    29521 caggcatcaa taaagtgcca tccatatcaa atgctgccag acgagccatt tctttctcca
    29581 ctctgtgaca cggttaatgg tgattgagta tcacctgata tatacggaag taatagtgaa
    29641 tagttaaata atattgttcc gggtttatat gcgattgctc aaccgtctta accagtatca
    29701 acgtctgtgg caaccttcag ccggaaagcc gcaaaccgtc accgtcagcg aactggccga
    29761 acgctgtttt tgcagcgaac gccatgttcg tacgctgttg cgtcaggcac aggaggcggg
    29821 atggctggag tggcaggcgc agtcaggacg cggaaagcgc ggacaattac gctttctggt
    29881 cacgccggaa tcgctacgca atgcgatgat ggaacaggca ctggaaaccg gaaagcagca
    29941 agatgtgctg gagctggcgc aactggcccc aggtgagctg cgcactctgt tacagccgtt
    30001 tatgggcgga caatggcaaa acgatacacc cacgttgcgt attccctact atcgcccgct
    30061 cgaaccgcta caaccaggct ttttgcccgg ccgtgccgag cagcatctcg ccgggcagat
    30121 attttccggc ctgacccgct tcgataataa tactcagcgc ccgattggcg atttagcgca
    30181 tcactgagaa acctctactg acgggttacg ctgggacttt tatcttcgtt caaccctaca
    30241 ctggcataac ggcgatgcag taaaagcctc acacttacac cagcgattat tgatgctgtt
    30301 acaactgcca gcactggatc aattatttat tagcgtgaag cgtattgaag tcacccatcc
    30361 gcagtgtctg accttctttt tacatcgccc cgattactgg cttgcgcacc ggctggcgag
    30421 ctattgcagc catctggcgc atccgcaatt cccactgatc ggcactggtc cttttcgctt
    30481 aacacaattc acagcggagc tggtgcgcct ggagagccat gattattacc atttgcgtca
    30541 tccgctgctt aaagcggttg agtactggat aactccgccg cttttcgaaa aagatttggg
    30601 aaccagttgt cggcatcccg tgcaaatcac catcggcaaa ccggaggagc tgcaacgggt
    30661 cagccaggtc agtagtggca tcagtttagg tttttgctat ttaacgttgc gcaaaagtcc
    30721 ccgactctcc ctctggcagg cgcgaaaagt gatctccatt attcatcaat ccggtttatt
    30781 acaaacgtta gaagtcggag aaaacctgat caccgccagt catgcattac tgccaggctg
    30841 gactattccg cactggcaag taccggatga agtcaaacta ccgaaaacct tgacgctggt
    30901 ttatcaccta ccgatagaac ttcataccat ggcagaacgc ctacaggcga cactggcagc
    30961 ggaaggctgt gaactcacaa ttatttttca taacgcaaaa aactgggacg acacgaccct
    31021 actggcacac gcagacctca tgatgggcga cagattaatt ggcgaagcac cggaatatac
    31081 tctggagcaa tggctacgtt gcgatccact atggccacat gttttcgacg ctccagcata
    31141 tgcacatctg caatcgacgc tggacgcggt gcaagtaatg cctgatgagg aaaaccgatt
    31201 taatgccctg aaagcggttt ttagccagtt aatggcagac gcgacgctga cgccgctgtt
    31261 caactatcac tatcgcatta gtgcccctcc cggcgtgaac ggtgtgcgac tgacaccgcg
    31321 cggctggttt gaatttaccg aagcctggct tcccgcgccg tcgcaatgaa tagctggtcc
    31381 gggttaatcg caggcgttac cataacgctt ttattgttca agcaggatta tctatgaaac
    31441 gtgctgtcgt tgtgttcagt ggaggccagg attccaccac ctgtctggtg caggcattac
    31501 aacaatatga tgaagtccat tgcgtgacgt tcgattacgg tcagcgacat cgcgcagaaa
    31561 tcgacgtggc acgcgaactg gcgctgaaac tgggagcacg cgcgcataag gttcttgatg
    31621 taacgctgct caacgagctg gcggtcagca gcctgacacg tgacagcatt ccggtgcctg
    31681 attatgaacc tgaagccgat ggcatcccga atacgtttgt ccctgggcgt aatattttgt
    31741 tcctgacgct ggcggcaata tatgcgtatc aggtaaaagc agaagccgta attaccggcg
    31801 tctgtgaaac ggatttctcc ggctacccgg attgccgcga tgagtttgtg aaagcactaa
    31861 accatgccgt cagtttgggc atggcgaaag atattcgttt tgaaacgccg ctgatgtgga
    31921 ttgataaagc ggaaacctgg gcgctggcag attattacgg caaactggat ttagtccgta
    31981 acgaaacgtt gacctgctat aacggcatta aaggcgacgg ttgcggtcat tgtgcggcat
    32041 gtaatttacg cgccaacggt ttgaatcatt atctggccga taaaccgacg gtgatggcag
    32101 cgatgaagca gaaaaccggg ttgaggtaat tattccgggt gtcgccggat gcggcttgag
    32161 catccggcac cacaaaacgt ttacttaacc atctgctcca gcttttcgcg caattcccct
    32221 tccagagcta atgctttctg cgttttaaga tcaatacaaa caaacgtaat aagcgcatcc
    32281 gctaccacct gcccttccgg ctccagtgta atgacctggc ttaagatgcc gcttttaccg
    32341 tttaattgct gcaactgact ggtaatagtt aacaggtcac ttaataccgc tgggcgacga
    32401 tagttaatat tgatattgac cacgacgaag gcgatgttat gggccgtcat ccactgaaaa
    32461 ctgtcgctat tttccaaccc atcccagcgg gcttcttcga gaaattcaag gtagcgggcg
    32521 ttgttgacgt gctggtaaac gtcgagatga tatccacgaa ctttgatttg tgtttgcata
    32581 gcgcaataac cttacgttgt tgttataagt acagaggtca taactggtat gacctcttca
    32641 gtctggcaaa aaattgccac tatgcaaatt aattacaggg ttaataccgc cagattacgt
    32701 tccaccagcg aattgcccat ccccggcacc tgctttagat cctccaccgt tttaaacgga
    32761 ccgtactctt cgcgataact gacaatcgcc tgcgctttct tcaggccaac gccattcatc
    32821 gcgcgggcta gctcttccgc gctggcatta ttaatgctga cccgggtgcc ttcttcgtca
    32881 ctggctttcg ccggtactgc tgctttactt tgtgctgcag gagcttccgc tttggtttct
    32941 accgttgtcg gtttcgccac agaagctgcc gccagcgcgc tatgagacat tccggcacag
    33001 gccagggaca gggtaatgag cagtgcttta attccgtgtt tcatactgtt ttctccttgt
    33061 ttgttaacag tggaatcacc ttaacggcag tgaaaacagt aaacaaatgg caatgttcag
    33121 aaatggaaaa ggccgcgaaa gcgacctttt cttgttacag ctgcaacgca tttttttgcg
    33181 aggcggcttc aggattattg ctgttccagc gcatcgccaa ttttgatttt cgcctcttta
    33241 cgcaggttac tcatcagagc ttcaaagacg atttgtgcgt tgttctgggt gataccctgc
    33301 accatcgctt ttttctgatc ttccggcatt gaaccttgtt tcacttcatc cagcgccagc
    33361 agaaccacat taccttgcat atcggtcgcc ataccgtagc tcggtttgtc tttcgctggc
    33421 agtggcagtg caaacgccgc ctggctaatc gggtcacgac cggaacggct taagattttc
    33481 ggctcgccaa atttcagacc ggcagcctgc atagcttccg cacctttgcc ggctttcaaa
    33541 tcaaccagca gtttctcagc atccactttc gcctgttgtt cagctttgtt gtgctgaacc
    33601 agtgccttaa cttgttcctg aacatctgcc aacggtttca ccgcttccgg tttgtgctcg
    33661 ctgatgcgca gcacgaatgc gcggtcgccg tctacggtga tgatgtcaga gttgatgccc
    33721 ggcgcgccgt tttcacctac cagaccgccg ttaaagatag cgtcggcaac cggcttgaag
    33781 ttcaactctt ccggcaggtt atctttgcta aaccaacccg tctgagtggc tttaacgccg
    33841 gcagcttgct ctgcaccggc cagagactcg gtgtcgttgc ttgccgcatc gctcactttc
    33901 tgctgcagcg catagtacgc atcgagggct ttttcgtgtt tcactttcgc cgcaatgtcg
    33961 tcacgtactt cgtctaacga tttcactttc gctggctgaa tgtcgtccag acgtacaatc
    34021 aggaaaccga ccgaagattt gatgacacca gacagttgcc ctttttcttt cagaccagca
    34081 tttttcagtt cgtcggggat agtggcatct tctaaccaac ccatatcgcc gccgttacgg
    34141 gcagagataa tatcggcaga tttttctttg gctaatgcag caaaatcacc gccttcattc
    34201 agctcatcaa gtaccgcttt cgcttcatct tcagttttgg tctggatgat gctgtagcgg
    34261 gtacgctgcg gctgggtgaa ttgatcctga tgctgatcgt agtagctctg gatatccgca
    34321 tcgctaaccg gttgctgcat cgttgcggca tccagcttga tgtaactcac gcggaattgt
    34381 tccggcgtca tgaaattgtt tttgttttgt tcgtagtagc tggcaatctc ctgttcggtc
    34441 acaggctgct tcgccgccag cgcgttaaca tcgatagtcg cctcacgcac cacgcgttgt
    34501 tgcgcgacca gtgccgccag ctcgtcggtt tcacctttca gcataaaatc ggtaccggca
    34561 acaccgttaa tcagctgttg ggtagtgagc tggttacgca gcgcctgggc gtactgatcg
    34621 gcggtcatcc ccatctggtt gaggataccg ttatagcggc tgttatcaaa tttgccgtca
    34681 acctggaagg ctggggtcgc gaaaatcgcc tgtttaacct gctcatcact gatacccagt
    34741 ttcagctcac gagcgtactg atccaacagc gcctcgtcga tcagacgatt cagcacctgt
    34801 tgacgcaggg ttttcatata gccttcgttc gctgccagct cggagtattg atcgcccagc
    34861 tgttgctgca tgcgattacg ctcgctgtta aaggcgttct cgaactgccc acggctgatt
    34921 tcctggtcat tcacttttgc ggcgtagtta ttgcctccgc caatcaggta accactcacg
    34981 ccggtcaata tgaacgacac gataatgata ccgaaaataa tcttgagcac gagactgttt
    35041 gcagccgtgc gtaagctgtc catcatggtg taacaacact ccgctgtaga tgactgtgta
    35101 cctcacgcaa cgtatcgatg cgcgcaaagg cctattgtga caagaaacgg gggcaattgt
    35161 cagcccacaa gacgcagaaa gtcaccgaat acaaataaaa aaggcacatc agtagatgcg
    35221 cccttgaact tcgtcacatc cccaccgggg acaacgctta gtttaccgcg tctttcagtg
    35281 ctttacctgc acggaagctc ggtactttag cagcagcgat ggtgatctct ttaccggtct
    35341 gcgggttgcg gccagtacgg gcagcacgct ctttaacggc aaaagtacca aaacctacca
    35401 gtgctacatc atccccttct ttcagagatt cagttacgga agcaataata gcatctaacg
    35461 cacggccagc cgcagcttta gagatatcag cccctgcagc aatcttgtcg atcaattgag
    35521 atttattcac tcttctcttc ctctttataa tttatatcgc acttgaatcc ttcaaggtac
    35581 gaacgcgcag cagttatatc aggccagcca tccccttaca acacccctta ataagggcaa
    35641 gcccgatccg ccatctaact tagcgagaca aaaaaaggct ggcaagcccg aattagcctg
    35701 ccagccctgt ttttattagt gcattttgcg cgaggtcact attttgcagt cacaacctgc
    35761 ataccagacg gttcattttg tagcgccaga gtcagaactt cctcaatgcg cttcacagga
    35821 tgaatgtcca gatcggcaat tacgttgtca ggaatctctt ccagatcgcg tttattttcg
    35881 aacggaatta gcactgtttt aatcccgccg cgatgcgctg ccaggagttt ttctttcaaa
    35941 ccaccgatcg gcagtacctg accacgcaga gtgatctcac cggtcattgc cacatcggca
    36001 cgaaccgggt taccggtcag gcaagaaacc agcgcggtgc acatagcaat accggcactc
    36061 ggaccatctt tcggcgtcgc accttccgga acgtggacgt ggatgtcacg tttttcgtaa
    36121 aaatcagggt tgatccccag tttttccgca cgcgcacgaa ccaccgttaa cgccgcctga
    36181 atagactcct gcatcacttc gccgagcgaa ccggtatagg tcagtttgcc tttacccgga
    36241 acacatgcgg tttcaatggt cagcaagtca ccgcccactt ccgtccacgc cagaccggtt
    36301 acctgaccga cacggttttc gttatcagcg cgaccatagt cgaaacgctg aacaccgagg
    36361 tagtcatgca ggttatcgcc gttaatttcg atatgtttta atgacttatc gagcagtaac
    36421 tgcttaaccg ctttgcggca cagtttggag atttcacgct ccagaccacg cacgcccgcc
    36481 tcacgggtgt agtaacgaat aatgccgata atggcgctat cgtcgacggt cagctcacct
    36541 tttttcagtg cattacgttc aatctgcttc ggcagcaggt gacgtttggc gatgttcagt
    36601 ttttcatctt cggtataacc ggagaggcga atcacttcca tacgatccag cagcggtgcc
    36661 ggaatgttca tggagttcga cgtcgcgaca aacatcacgt cgctgagatc gtaatccact
    36721 tccaggtagt ggtcgctgaa cgctacgttc tgctctggat ccagcacttc aagcagtgca
    36781 gaggccggat cgcctcgcat gtcagaagac attttgtcga tctcatcgag caggaacagc
    36841 gggtttttca cgcccacttt cgccattttc tggatcagtt tacccggcat agaaccgatg
    36901 taagtacggc ggtgaccacg gatttccgct tcatcacgca cgccgcccag cgccatacgg
    36961 acatatttac gcccggtggc tttggcaatg gactgaccaa gagaggtttt acctaccccc
    37021 ggcggcccta ccaggcagag gatcggtccc ttgattttgt tgacacggct ttgaaccgca
    37081 aggtactcaa ggatgcgatc tttcacgcgc tccagaccat aatggtcggt atcaaggatt
    37141 tcctgcgcct gacgcaggtc ttttttgacc ttgctgcgcg cattccacgg tacctgtacc
    37201 atccagtcga tataaccacg cactacggtc gcttctgccg acatcggaga catcattttc
    37261 agcttctgca actctgcttc cgctttctct tttgcctctt tcggcatctt cgccgcgtcg
    37321 attttgcgct tcagggcttc gttttcgtcc ggcgcgtcgt ccatttcacc gagttctttc
    37381 tgaatagctt tcatttgctc gttcagatag tactcacgct gggatttctc catctgcttt
    37441 ttaacgcggt tgcgaatgcg tttctcaacc tgcagcagat cgatttccga ttccatcatt
    37501 gccatcagat attccagacg ttcgttaacg tcggacatct ccagaacgga ctgtttgtca
    37561 gccagtttca gcggcatatg tgcagcaatg gtatccgcca gacgcgccgg atcgtcgatg
    37621 ctattcagcg acgtcagcac ttctggtggg atttttttgt tcagcttgat gtagccttcg
    37681 aactggctga ttgcagtacg caccagcact tcctgttccc gctcatcaat ggtcggcgac
    37741 tccagatact ccgccttcgc agaaaagtgt tcgccattgt cagagagcgc agaaatacgc
    37801 gcgcgctgta acccctcgac cagcactttg acggtgccgt caggcagttt cagcatctgc
    37861 aatatagagg ccacggtccc gacggtgaaa agatcgttta cacccggctc atccgttgaa
    37921 gcttctttct gcgcgaccag cataattttt ttatcatggt ccatcgccgc ttccagacaa
    37981 cggatagatt tttcccgccc gacaaataag gggatgacca tgtgcggata aaccaccaca
    38041 tcgcgcagcg gcaatacggg gatttcaatg cgttcagaac gctcaggatt catagagctc
    38101 tctcttagtt taatttccgc caggtaatca gatgacacga ctgtgcttca cgccatctat
    38161 taacatgtac gtcagtatat ggggatgttt cccccacatt caacgccgag aatagaggaa
    38221 aaattaaagg ggagataaaa tccccccttt ttggttaact aattgtatgg gaatggttaa
    38281 ttattcacca gatgcctgtt gcgcttccgg cttgccataa atcagcaacg gtttgctttg
    38341 accatcaatt accgactcgt cgataaccac tttttcgaca tcttccatgg acggcagatc
    38401 gtacatggta tcgagcagtg cggcttctac gatggaacgc aggccacggg caccggtttt
    38461 acgcgccatc gctttcttag cgatagcatc cagcgcctcg tcacggaatt ccagatccac
    38521 gccttccaga ttaaacagcg cctgatactg cttggtcagg gcgtttttcg gctctttgag
    38581 gatctgaatc agagcttctt cgctcagttc attcaacgtt gcgacaaccg gcagacgacc
    38641 aatgaactca gggatcagac caaacttgat cagatcttcc ggttcaacct gcgccagcag
    38701 ctcgccttcg cttgctttgt cggacttcgc ttttaccgtc gcgccaaaac caatgccgga
    38761 gccggtttct acacgatggg aaatcacttt atccagaccg gcaaacgcac cgccacaaat
    38821 aaacaggatc ttagaggtat caacctgcaa gaattcctgc tgcggatgtt tacgcccacc
    38881 ttgcggtgga acagcagcta ccgtaccttc gatcagtttc aacagtgcct gctgtacgcc
    38941 ttcaccggaa acgtcacggg taatagacgg gttgtctgac ttacgagaaa tcttgtcgat
    39001 ttcatcgatg tagacaatcc cgcgctgcgc tttctgtacg tcgtaatcgc acttttgcaa
    39061 cagcttctga atgatgtttt caacgtcttc gcccacataa ccggcttcgg tcagcgtggt
    39121 tgcgtcggcc atggtgaacg ggacgtccag caggcgcgcc agcgtttcag ccagcagcgt
    39181 tttaccggaa ccggtcggac cgatcagcag aatgttactt ttgcccaact cgacaccatt
    39241 gctggtatcg ccgttgcgca gacgtttgta atggttgtat accgcgaccg ccagcacttt
    39301 tttcgcctgt tcctggccga taacgtaatc gtccaggtgg ttgcgaattt catgcggcgt
    39361 cggtagcgca ctgcgttcac gatgcggtgc aacttcttta atctcttcgc gaatgatgtc
    39421 gttacataaa tcaacacatt cgtcgcagat atacacggat ggaccggcaa tcagcttgcg
    39481 cacttcatgc tggcttttgc cgcaaaaaga gcaatacagc aatttgcctg agccatcttt
    39541 gcgtttatct gtcatgggtc aaaacctctt ctttgttctt tgtgccgcac acgacgacgc
    39601 aaatgccatt ctcaggcgca agccgcttac agcgttgtgc cgccctggat aagtatagcg
    39661 gcacagttgc gcctctggca tcaattacga tgggtcagaa tcgaatcgac cagaccgtat
    39721 tccaccgctt caggggcgga aaggaagcga tcgcgctcgg tatcacgttc aatctgttct
    39781 aatgattgac ccgtatgaag cgccataagt tcattcatgc gccctttaac tttcagaatt
    39841 tcacgggcat gaatttcgat atcggtcgcc tggccctggt agccgcccaa cggttggtga
    39901 atcatcacgc gcgaattcgg caggcaaaaa cgtttacctt ttgcccctgc ggtcagcaag
    39961 aaagcgccca tcgaggccgc ctggcccata cagatggtgc tgacatcagg cttgataaac
    40021 tgcatggtgt catagataga catcccggca gtgatcaccc cgcctgggga gttaatgtac
    40081 agatagatat ctttttctgg gttttccgct tccaggaaca gcatctgcgc cacaatcagg
    40141 ttagccatgt ggtcttcaac ctggccagtc agaaaaatga cgcgttcctt aagtagacga
    40201 gaatagatat caaaagagcg ctcaccgcgt gaggtctgtt caatgaccat cggcaccagc
    40261 gccatatggg gtgcaaagtt atctcgttcg ccgctgtatg acatttccgt ctcctggata
    40321 aaattgaaaa aacctgctgt accgattgta accttatgga cggattatca gctagtccct
    40381 gtgttatggg tacggcatca ccatatttca agcataacaa tcttttgttg ttacgctaac
    40441 accgaaagag gcttttcgca cggttccatg caaaattcat gacaaaaaaa agcccaccac
    40501 ctggcggtga cgggcctttg tgcgaattta gcgcgttatg ctgcgtaaat tacgcctgct
    40561 ggttcatcag ctcgttgaaa gtggtttctt tttcagtcac tttcgctttc gccagtacag
    40621 cttcaacagc ctgttcttcc agagcaacat tgcgcatgtt gtccatcagt tctttgtttt
    40681 tgctgtagaa ctcgataact tctttcggat cttcgtacgc agaagccatc tcttcgatca
    40741 ggcctttcac gcgctcttcg tcagctttca gctcgttggt gcggataact tcgcccagca
    40801 gcaggccaac aactacgcgg cgtttagcct gttcttcgaa cagttcgcgc ggcagttcca
    40861 gagcttgttt ttcgttgcca ccgaaacgct gtgcagcctg gcgacgcaga acgtcgattt
    40921 cgctgtcgat cagcgcagcc ggtacgtcga tgtcgttagc ttttaccaga ccttcgatcg
    40981 cctgagactt aacgcggtta cggatggcgc tcttcagctc gcgctccatg tttttacgca
    41041 cttcagcgcg cagaccttct acggaaccat cttcaacgcc gaaacgtttg atgaattctg
    41101 cagtcagttc cggcagttca cgctcttcaa ctttcttcag gttgatagcg aatttcgctg
    41161 ctttaccttt caggttttct gcgtggtatt cttccgggaa ggtcacgtcg atggtgaact
    41221 cttcgccagc tttgtggcct ttgataccgt cttcaaagcc cgggatcata cgaccctggc
    41281 ccatcgccag tacgaaatca gacgctttac cgccttcgaa ctcttcgccg tctacagaac
    41341 cggtgaagtc gatggttacg cggtcttctg cttcaacagc gccgtctttt tctttccagg
    41401 tcgcctgctg tttacgcaga gtatccagca tgccgtcaac gtcagcgtcg gtcacttcaa
    41461 cgatcggttt ttcaacttcg atcgcttcca gaccctgcag ttcaacttcc ggataaactt
    41521 caaactctac agagtaagtg aagtcttcac ccagcttgta ttcgcccgga acataagtcg
    41581 gtgcgccagc cggattgatt ttttctttaa tgatggcgtc aatgaagtta cggctcatca
    41641 ggtcacccag aacgtcctgg cgtacagacg cgccataacg ctgagcaacg atattcattg
    41701 gcactttgcc tttgcggaag ccgtcaatac gtactttttt cgcaacgttg accagctcgc
    41761 ttttaacagc ggtctcgatg ctgtcagcag cgatagtaat cgttacacgg cggccaaggc
    41821 cttgagtggt ttcaactgaa acttgcatct tgttacctca aaaaatcaca gtgctcggtc
    41881 aactcttacc ccgcaagcac aggttgttgg cttcgcggaa ccgggatgtt cttataatca
    41941 atcacattcc ctgttaccag aatcatcccg aaagcattca aataggacgc ggcattatag
    42001 cggcatcact tttatgagtc gagaacggtt attgcgcgct gtcgcgtatt ttttcacaat
    42061 tcccgggcaa ttttcgtctg aatgctgggc aaaaagcgca aaattcagac aaaacaaaac
    42121 ggcccgtagg ccgtttttca taaaatcttt agcaacatac tggaaaagcg ccgacagttg
    42181 caaatgcgtt tttacgcgat gcttcctgct ccacgacagg gaggagaggc aaagacggtg
    42241 tcctgcaacc cttcccactc cttaatagtg taagtatgca gagccagcgc atgaacggta
    42301 gtagagagtt cctccgctaa agtactgtaa atcattcgat gacgattcag aaaacgttca
    42361 cccgtaaaac gatcgctgac cagcacaact ttaaaatggc tttcagagcc ggctgggaca
    42421 ttgtgacgat agctttcatc cactacttcg aggaatacgg gttggaacgc cgcccttaat
    42481 ttttcttcta tccgctcacg tatcatcatg aaatatctcc tccgacaacg ctgagatgtc
    42541 atccatccct ttaaatacta gccgctttta ccgtttccat tgcagcttaa caacaaatat
    42601 ttaggtttcg tcctggtttt tccgtcaagc gtatgaagtt tactgattta aagattgaaa
    42661 cacaggcctc gcatcagtgt ttcttttgcc aggggcgatt atttcgtcaa catgatgaat
    42721 ttacatgcct tacccacttc ccctcgccgt tggcaatgtt atgatggcgg gaatttttac
    42781 caaacacgct taagagtctc tgagagcccg aacatgttca aaaaaatcct cttcccgtta
    42841 gttgctctgt ttatgcttgc aggatgcgca aaaccgccaa caactattga agtttccccg
    42901 acgattacac tgccacagca ggatccaagc ctgatgggcg tcaccgtaag cattaatggt
    42961 gccgatcagc gtaccgatca ggcgctggca aaagtcaccc gcgataatca aatcgttacc
    43021 ctgaccgcct cccgcgatct gcgtttcctg ctgcaagaag tgctggaaaa acagatgacc
    43081 gcgcgtggtt acatggttgg tccgaatggc ccggttaatc tgcaaatcat cgttagccaa
    43141 ctgtatgctg acgtgtccca gggcaacgtg cgctataaca tcgctaccaa agcggatatc
    43201 gccatcatcg ctaccgcgca gaatggcaac aaaatgacca aaaactatcg tgccagctac
    43261 aacgttgaag gtgcgttcca ggcctccaac aaaaatatcg ccgatgcggt taacagtgtg
    43321 ctgaccgata ccatcgctga tatgtctcag gacaccagca ttcacgaatt tatcaagcag
    43381 aacgcgcgtt aatttctgcc ctctggcccg gtgcaaaccg ggcctgtaga cgcccatgtc
    43441 cagtcaatat ttacgtattt ttcaacagcc gcgttcagcc atattgctga tcctgggttt
    43501 tgcttccggg ctaccgctcg ccctgacatc cggtacctta caggcctgga tgacggtcga
    43561 gaatattgat ctcaaaacca ttggtttctt ctctctggta ggccaggctt acgtttttaa
    43621 attcctctgg tcaccgctga tggaccgcta cacgccccca ttttttgggc ggcggcgcgg
    43681 ttggctgctc gccacgcaaa tcctgttatt agtcgccatt gcggcgatgg gttttctcga
    43741 acctggcacc caactccgct ggatggcggc actggcggtg gtgatcgctt tttgctcggc
    43801 ctcccaggat attgtcttcg atgcgtggaa aaccgatgtg ctcccggcag aagaacgtgg
    43861 tgcgggcgcg gcaatcagcg tgctgggtta ccgtttaggg atgctggttt ccggcggctt
    43921 agccctgtgg ctggcagata aatggctggg ctggcagggc atgtactggt tgatggcggc
    43981 gctgttgatc ccttgtatta tcgcgacgtt gcttgcacca gaaccgaccg acaccattcc
    44041 tgtgccaaaa acgctggaac aagcggttgt tgcacctctg cgagatttct ttggtcgcaa
    44101 taatgcctgg cttattttgc ttcttatcgt gctgtataag ctgggcgacg cattcgccat
    44161 gagcctgaca accacgtttt tgattcgcgg cgtcgggttt gatgcgggtg aagtaggcgt
    44221 ggttaacaaa acgcttggct tactggcgac cattgtgggc gcattgtacg gtgggatttt
    44281 gatgcagcgc ctgtcgctgt tccgggcgct gctgattttc ggcattttac aaggtgcgtc
    44341 taacgctggt tactggctgc tgtcgattac tgataagcat ctctacagca tgggcgcagc
    44401 cgtctttttc gaaaacctct gtggcgggat gggcacatca gcctttgtcg cgctgttaat
    44461 gacgctgtgt aataagtcat tttccgcaac tcaatttgcc ctgctctcag cgctttccgc
    44521 tgtaggacga gtttatgtcg gccccgtggc gggttggttt gttgaagcac acggctggtc
    44581 gacattctat ctattctccg tcgccgctgc cgtaccgggg cttattttgc tgctggtttg
    44641 ccgccagacg cttgaatata cacgagtaaa tgacaacttt atctcccgta ccgaatatcc
    44701 ggcaggttat gcctttgcca tgtggacact ggcggcgggc gtcagcctgt tggccgtgtg
    44761 gttactgctg ttgacgatgg acgcgctgga tttgacgcac ttctctttcc tgcctgctct
    44821 gctggaagtc ggggttttag tcgccctttc tggcgtcgtg cttggtggtt tgctggatta
    44881 tctggcgcta cgaaaaacgc atctgacgta atctgtaaat attatttagc tgtagtaatc
    44941 actcgccgta attattacgg ctaaataaac atcagtatcg cttaataata tttgttcttt
    45001 tgtgcggctt agcgtttgga aattattgac gc
//
LOCUS       DAZFXT010000024        43321 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.024, whole genome shotgun sequence.
ACCESSION   DAZFXT010000024 DAZFXT010000000
VERSION     DAZFXT010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 43321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43321
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.024"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..59)
                     /locus_tag="ACHKW0_001455"
     tRNA            complement(1..59)
                     /locus_tag="ACHKW0_001455"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31..33),aa:Met,seq:cat)
     gene            276..1373
                     /gene="mltA"
                     /locus_tag="ACHKW0_001456"
     CDS             276..1373
                     /gene="mltA"
                     /locus_tag="ACHKW0_001456"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HFO7474314.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1450..2256
                     /gene="tcdA"
                     /locus_tag="ACHKW0_001457"
     CDS             1450..2256
                     /gene="tcdA"
                     /locus_tag="ACHKW0_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HFO7474315.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2307..2750)
                     /gene="csdE"
                     /locus_tag="ACHKW0_001458"
     CDS             complement(2307..2750)
                     /gene="csdE"
                     /locus_tag="ACHKW0_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HFO7474316.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TVAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(2750..3955)
                     /gene="csdA"
                     /locus_tag="ACHKW0_001459"
     CDS             complement(2750..3955)
                     /gene="csdA"
                     /locus_tag="ACHKW0_001459"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HFO7474317.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4147..4374
                     /gene="ygdI"
                     /locus_tag="ACHKW0_001460"
     CDS             4147..4374
                     /gene="ygdI"
                     /locus_tag="ACHKW0_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HFO7474318.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            4725..5642
                     /gene="gcvA"
                     /locus_tag="ACHKW0_001461"
     CDS             4725..5642
                     /gene="gcvA"
                     /locus_tag="ACHKW0_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HFO7474319.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5661..6056
                     /gene="ygdD"
                     /locus_tag="ACHKW0_001462"
     CDS             5661..6056
                     /gene="ygdD"
                     /locus_tag="ACHKW0_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HFO7474320.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6049..7149
                     /gene="rlmM"
                     /locus_tag="ACHKW0_001463"
     CDS             6049..7149
                     /gene="rlmM"
                     /locus_tag="ACHKW0_001463"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HFO7474321.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7193..7924)
                     /gene="fucR"
                     /locus_tag="ACHKW0_001464"
     CDS             complement(7193..7924)
                     /gene="fucR"
                     /locus_tag="ACHKW0_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HFO7474322.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(7982..8404)
                     /gene="fucU"
                     /locus_tag="ACHKW0_001465"
     CDS             complement(7982..8404)
                     /gene="fucU"
                     /locus_tag="ACHKW0_001465"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016854 - racemase and epimerase activity
                     [Evidence IEA];
                     GO_function: GO:0042806 - fucose binding [Evidence IEA];
                     GO_process: GO:0042354 - L-fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HFO7474323.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8406..9824)
                     /gene="fucK"
                     /locus_tag="ACHKW0_001466"
     CDS             complement(8406..9824)
                     /gene="fucK"
                     /locus_tag="ACHKW0_001466"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008737 - L-fuculokinase activity
                     [Evidence IEA];
                     GO_process: GO:0042355 - L-fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO7474324.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            complement(9933..11708)
                     /gene="fucI"
                     /locus_tag="ACHKW0_001467"
     CDS             complement(9933..11708)
                     /gene="fucI"
                     /locus_tag="ACHKW0_001467"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008736 - L-fucose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019317 - fucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HFO7474325.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11741..13057)
                     /gene="fucP"
                     /locus_tag="ACHKW0_001468"
     CDS             complement(11741..13057)
                     /gene="fucP"
                     /locus_tag="ACHKW0_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO7474326.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13604..14251
                     /gene="fucA"
                     /locus_tag="ACHKW0_001469"
     CDS             13604..14251
                     /gene="fucA"
                     /locus_tag="ACHKW0_001469"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008738 - L-fuculose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006004 - fucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HFO7474327.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14279..15427
                     /gene="fucO"
                     /locus_tag="ACHKW0_001470"
     CDS             14279..15427
                     /gene="fucO"
                     /locus_tag="ACHKW0_001470"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HFO7474328.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15482..16237)
                     /gene="xni"
                     /locus_tag="ACHKW0_001471"
     CDS             complement(15482..16237)
                     /gene="xni"
                     /locus_tag="ACHKW0_001471"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0017108 - 5'-flap endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0033567 - DNA replication, Okazaki fragment
                     processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HFO7474329.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16349..17716)
                     /gene="sdaB"
                     /locus_tag="ACHKW0_001472"
     CDS             complement(16349..17716)
                     /gene="sdaB"
                     /locus_tag="ACHKW0_001472"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HFO7474330.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17774..19063)
                     /gene="sdaC"
                     /locus_tag="ACHKW0_001473"
     CDS             complement(17774..19063)
                     /gene="sdaC"
                     /locus_tag="ACHKW0_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HFO7474331.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            complement(19620..20984)
                     /gene="ppnN"
                     /locus_tag="ACHKW0_001474"
     CDS             complement(19620..20984)
                     /gene="ppnN"
                     /locus_tag="ACHKW0_001474"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HFO7474332.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            complement(21096..21944)
                     /gene="queF"
                     /locus_tag="ACHKW0_001475"
     CDS             complement(21096..21944)
                     /gene="queF"
                     /locus_tag="ACHKW0_001475"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Catalyzes the NADPH-dependent reduction of
                     7-cyano-7-deazaguanine (preQ0) to
                     7-aminomethyl-7-deazaguanine (preQ1) in queuosine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA];
                     GO_function: GO:0043867 - 7-cyano-7-deazaguanine
                     tRNA-ribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HFO7474333.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            22012..22557
                     /gene="syd"
                     /locus_tag="ACHKW0_001476"
     CDS             22012..22557
                     /gene="syd"
                     /locus_tag="ACHKW0_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HFO7474334.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            23179..23508
                     /gene="yqcC"
                     /locus_tag="ACHKW0_001477"
     CDS             23179..23508
                     /gene="yqcC"
                     /locus_tag="ACHKW0_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HFO7474335.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLDNKQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            23508..24290
                     /gene="truC"
                     /locus_tag="ACHKW0_001478"
     CDS             23508..24290
                     /gene="truC"
                     /locus_tag="ACHKW0_001478"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HFO7474336.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            24308..24757
                     /gene="yqcA"
                     /locus_tag="ACHKW0_001479"
     CDS             24308..24757
                     /gene="yqcA"
                     /locus_tag="ACHKW0_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HFO7474337.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            25192..26544
                     /gene="gudP"
                     /locus_tag="ACHKW0_001480"
     CDS             25192..26544
                     /gene="gudP"
                     /locus_tag="ACHKW0_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HFO7474338.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            26546..27886
                     /gene="gudX"
                     /locus_tag="ACHKW0_001481"
     CDS             26546..27886
                     /gene="gudX"
                     /locus_tag="ACHKW0_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HFO7474339.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            27907..29247
                     /gene="gudD"
                     /locus_tag="ACHKW0_001482"
     CDS             27907..29247
                     /gene="gudD"
                     /locus_tag="ACHKW0_001482"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008872 - glucarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HFO7474340.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(29479..32235)
                     /gene="barA"
                     /locus_tag="ACHKW0_001483"
     CDS             complement(29479..32235)
                     /gene="barA"
                     /locus_tag="ACHKW0_001483"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HFO7474341.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            32292..33593
                     /gene="rlmD"
                     /locus_tag="ACHKW0_001484"
     CDS             32292..33593
                     /gene="rlmD"
                     /locus_tag="ACHKW0_001484"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HFO7474342.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            33641..35875
                     /gene="relA"
                     /locus_tag="ACHKW0_001485"
     CDS             33641..35875
                     /gene="relA"
                     /locus_tag="ACHKW0_001485"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP diphosphokinase"
                     /protein_id="HFO7474343.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            35953..36201
                     /gene="mazE"
                     /locus_tag="ACHKW0_001486"
     CDS             35953..36201
                     /gene="mazE"
                     /locus_tag="ACHKW0_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HFO7474344.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            36201..36536
                     /gene="mazF"
                     /locus_tag="ACHKW0_001487"
     CDS             36201..36536
                     /gene="mazF"
                     /locus_tag="ACHKW0_001487"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HFO7474345.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            36607..37398
                     /gene="mazG"
                     /locus_tag="ACHKW0_001488"
     CDS             36607..37398
                     /gene="mazG"
                     /locus_tag="ACHKW0_001488"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HFO7474346.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            37626..39263
                     /gene="pyrG"
                     /locus_tag="ACHKW0_001489"
     CDS             37626..39263
                     /gene="pyrG"
                     /locus_tag="ACHKW0_001489"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine hydrolyzing CTP synthase"
                     /protein_id="HFO7474347.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPECKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            39351..40649
                     /gene="eno"
                     /locus_tag="ACHKW0_001490"
     CDS             39351..40649
                     /gene="eno"
                     /locus_tag="ACHKW0_001490"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HFO7474348.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(40709..41581)
                     /gene="ygcG"
                     /locus_tag="ACHKW0_001491"
                     /pseudo
     CDS             complement(40709..41581)
                     /gene="ygcG"
                     /locus_tag="ACHKW0_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(41595..41735)
                     /gene="yqcG"
                     /locus_tag="ACHKW0_001492"
     CDS             complement(41595..41735)
                     /gene="yqcG"
                     /locus_tag="ACHKW0_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope stress response protein YqcG"
                     /protein_id="HFO7474349.1"
                     /translation="MSEENKENEFNHVKTFTKIIFIFSVLVFNDNESKITDAAVNLFI
                     QI"
     gene            41874..42545
                     /gene="queE"
                     /locus_tag="ACHKW0_001493"
     CDS             41874..42545
                     /gene="queE"
                     /locus_tag="ACHKW0_001493"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HFO7474350.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
     repeat_region   42885..43218
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=42885..42913
                     /rpt_unit_seq="gagttccccgcgccagcggggataaaccg"
BASE COUNT        10818 a        10432 c        11425 g        10646 t
ORIGIN      
        1 ggccggattt gaaccgacga ccttcgggtt atgagcccga cgagctacca ggctgctcca
       61 ccccgcgtcc gtggatgcgc actatactcc gctcgcgttt tcatgcaacc ttttttttac
      121 cccgatcacg gatttagcat gattttgaac aaaaacaaac cactaaagcc tattttttgt
      181 acaaaatttg cgtcagggag aggctgctca tttcattaaa cgcggcggtt tgttatcttc
      241 gttgcgcctt attttttaac ctgaagaaga gaacaatgaa aggacgttgg gtaaagtacc
      301 ttcttatggg cacggttgtg gcaatgcttg ccgcctgctc ttccaaacca accgatcgcg
      361 gacagcaata taaagacggg aaatttaccc agcctttctc tctggtgaac cagccagatg
      421 ccgttggcgc gccgattaac gccggtgatt ttgccgagca aattaaccat atccgtaatt
      481 cgtcaccgcg tctgtatggc aaccagagta atgtttataa cgcggtgcaa gagtggctgc
      541 gcgcaggcgg tgatacccgc aatatgcgcc agttcggcat tgatgcctgg cagatggaag
      601 gtgccgacaa ctatggtaac gtgcagttta caggctatta cacgccggta attcaggcgc
      661 gccatacccg ccagggcgag ttccagtatc ctatttaccg tatgccgcca aaacgtggtc
      721 gtctgccgtc tcgtgcggag atctacgcag gggcgctgag tgataaatat attctcgctt
      781 acagcaactc cctgatggat aacttcatta tggatgtgca gggtagcggg tatatcgact
      841 ttggtgatgg cagtccgctt aactttttca gctatgcagg gaaaaacggt catgcctatc
      901 gcagcattgg taaggtgctg atcgaccgtg gcgaagtgaa aaaagaagat atgtcgatgc
      961 aggcgattcg tcattggggc gaaacacaca gtgaagccga ggttcgcgag ctgctggaac
     1021 agaacccgtc tttcgttttc tttaaaccgc aatcttttgc accggtgaaa ggggcaagtg
     1081 cggtgccgct ggttggccgt gcttctgttg cctccgatcg ttcaattatt ccgccaggta
     1141 ctaccttgct ggcagaagtg ccgctgctgg ataataacgg caaatttaac ggtcagtacg
     1201 aattgcgtct gatggtggcg ctggatgtcg gtggcgcaat caaaggccaa cacttcgata
     1261 tctatcaagg gatcgggccg gaagccggac atcgcgcagg ttggtacaac cactatggac
     1321 gtgtctgggt gctgaaaacc gccccgggcg caggtaacgt ctttagcggc tgatgtgtta
     1381 ttctgtatac cgtttttacg gatacgccag ggtgagggat aacctcaccc tttttaattc
     1441 tgaggtgcta tgtctgtggt aattagtgat gcatggcgtc agcgttttgg tggcacagcg
     1501 cgtctgtatg gtgaaaaagc gttgcaactg tttgctgacg cgcatatttg tgtggttggt
     1561 atcggtggtg tcggttcctg ggcggcggaa gcgctggcgc gcacggggat tggcgcaatc
     1621 acgcttatcg atatggatga tgtgtgcgtc accaatacca atcggcaaat tcacgctctg
     1681 cgcgataacg tcgggctggc aaaagcggaa gtcatggcgg agcgtattcg ccagattaac
     1741 ccggagtgcc gtgtaacggt ggtggatgat ttcgtgacgc cggataacgt agcgcagtat
     1801 atgagcgtgg gttattcgta cgtgattgat gccattgata gtgtacggcc caaagcggcg
     1861 ctgattgctt attgtcggcg caataaaatc ccgctggtca cgaccggtgg cgcgggtggg
     1921 cagattgacc cgacgcagat tcaggttacc gatctggcga aaacgattca ggatccgctg
     1981 gcggcgaagt tgcgcgagcg cctgaaaagc gattttggcg tagtgaaaaa cagtaaaggt
     2041 aagctcggcg tcgattgcgt gttttctact gaagcgctgg tgtacccgca gtcagacggt
     2101 actgtgtgtg cgatgaaagc cacggcagaa gggccgaagc ggatggattg tgcatctgga
     2161 tttggcgcgg caacgatggt gaccgccacc tttgggtttg ttgcggtttc tcatgcgctg
     2221 aagaagatga tggcgaaagc ggcgcgtcag ggttaaacaa tcaggtcgga tgcgacgcga
     2281 acgtcttatc cgacctggtt ctgtgcttaa acctgcttcg ccgcagcgat aatcgcctcg
     2341 cttaacgcat tcaacccctg gctgcgtgag gcgctaagct gtgcacgcaa tcccagctca
     2401 tcaaacaatg ccagcggcga ctgtgcctgc aattcggcga cggttttccc ctcaacggca
     2461 gtcaacaaca ccgccaacag gccgcgcaca atgcgccctt cgctgtcgcc aaagaagtgc
     2521 attttaccgt tttcagtcac ggtatatccc agccagacgc ggttttcgca tccggcaatc
     2581 tctttagcct gcgcttttaa ctcgtctggc aaagccggaa gctgtttccc cagcatgatc
     2641 aactggcgat atttatcttc ccattgcgac aacggtgcga aggtattgcg taacgtttct
     2701 gcggttacgg ttgtgccgaa cggatgtccg gcgaattgcg ggtttgtcat taatccacca
     2761 ataattccag cgcgcggtca acggcattca ccagcgcatc cacatcactc tttgtattat
     2821 atggcgcaaa agaggcgcgc agtgtgccgg ttacgcctaa ttctgccagt agcggctgag
     2881 cgcaatgctg cccggcccgc agggcaatac cgtactccgc cagcagcgtc accatatcgc
     2941 tatgatgaac gccagcaaaa tcaaaggcca gcaggctgga atcctggcag cggaatgaac
     3001 gaaagccggg acgtttcgcc agcgcatctt ccgccagcgt tgctaagcta cggctccagc
     3061 tttcggcctg gttgatatcg taatctgcca gccattccag cgccgcgctt aatcctatga
     3121 caccagcgac atttggcgtt ccagcttcca gtttccacgg cgcagattga gtcgtgaagc
     3181 cgtcaaaact cacttcgtga atcattttgc cgccacccag ccagggcgac atcgcttcca
     3241 gcagttctga tttgccatac agcacgccga tacccgtcgg gccatacagt ttgtgaccag
     3301 agaaagcata gaaatcgata tctagttgct gaacatctgc ggggaaatgc accgccccct
     3361 gagcaccatc aaccatcacc accatcccgg ctgaatgagc aaaggtaatc gctcgcgcca
     3421 gatccgggca accgccagta acgttcgaca tctgacccaa cgccagaatc cgactacggg
     3481 gagtaatcag ttctggcaac aaatcgacat ccggcagtcg ctgcgcatta agcggcaatt
     3541 tcaccacttt ggctccagtt tgttgggcga ccatcagcca ggggacgagg ttagcgtggt
     3601 gttctgccac gctgacaata atctcatcgc ccggttgcag acgcggacgc gcatagcatt
     3661 gtgccaccat gttgatggat tcagtggtgc cgcgcgtcca gacgatagtt ttatcatccg
     3721 gtgcattcag taattgcgcc actttctctc gtgcagcttc ataacgcgcg gtcaggcgtt
     3781 gggcttcggc aaactggctg cgatggacgt ttccggcgct caggctgtaa aactgtcggg
     3841 tggcttcaac cacggcttca ggtttaagcg cggtcgcggc gctgtcgaga tagacgcccg
     3901 catcctgtag agcgggaaac tgggcgcgaa actgcgcggg attaaaaacg ttcatggtac
     3961 tcctcggctt gatgcaccga tcgtgacgca aaattcactt tgatacaagg agagtgctaa
     4021 ccatcagaat tatctggata tcctggcgaa gccgcgtaag taacatatgc tgaatagtgt
     4081 taggcgttaa ttttagcctg ttaatcaata ctattaagat ttattaattg ctttaaggag
     4141 aagatgatga aaaagactgc cgcaattatt tctgcctgta tgctgacttt tgccctgagc
     4201 gcctgttccg gttcgaacta tgtgatgcac accaatgacg gacgtaccat cgtctctgac
     4261 ggcaaaccac agactgataa cgataccggt atgatttcgt ataaagacgc taatggcaac
     4321 aaacagcaga tcaaccgtac tgacgtgaaa gagatggtcg aactggatca gtaattcacg
     4381 atcgcaaggt aaaaaaaagc accgcaatta ggcggtgcta cattaatcac tatggacaga
     4441 cagggtaaat gtacaggaag tgaaaaaagg tagctttgct accatggtct gaatcgcaga
     4501 ccaattgcaa acacaacaac acaacatcac aaccgtaagc caaaagttca ccagaacacg
     4561 cattccgata aaacttttcg ttccggctca ggaagtgccg ccactatagg tatttgctgg
     4621 tagaagctca acggacaatt tataatggct cagattaaaa aaactaatag gttacacagt
     4681 gtgatctaat tgttaaattc atttaacatc aaagtttaaa agccatgtct aaacgattac
     4741 caccgctaaa tgccttacga gtttttgatg ccgcagcacg ccatttaagt ttcactcgcg
     4801 cagcagaaga gctttttgtg acccaggccg cagtaagtca tcaaatcaag tctcttgagg
     4861 attttttggg gctaaaactg ttccgccgcc gtaatcgttc actcctgctg accgaggaag
     4921 ggcaaagcta tttcctcgat atcaaagaga tattttcgca attaaccgaa gcgacgcgta
     4981 aactccaggc ccgtagcgcc aagggggcgt tgacggtcag tttactcccc agtttcgcca
     5041 ttcattggtt ggttccgcga ctttccagct ttaattcagc ttatccggga attgacgttc
     5101 gaatccaggc ggttgatcgt caggaagata agctggcgga tgatgttgat gtggcgatat
     5161 tttatggtcg gggcaactgg ccggggctac gggtggaaaa actgtacgcc gaatatttat
     5221 tgccggtgtg ttcgccgcta ctgctgacag gcgaaaaacc cttgaagacc ccggaagatc
     5281 tggctaaaca tacgttatta catgatgcgt cacgccgtga ctggcagaca tatacccgac
     5341 agttggggtt aaatcatatc aacgttcagc aagggccaat ttttagtcat agcgccatgg
     5401 tgctgcaagc ggctattcac gggcagggag tggcgctggc aaataacgtg atggcgcaat
     5461 ctgaaattga agccggacgt cttgtttgcc cgtttaatga tgttctggtc agtaaaaacg
     5521 ctttttatct ggtttgtcat gacagccagg cagaactggg taaaatagcc gcctttcgcc
     5581 aatggatcct ggcgaaagcc gctgctgaac aagaaaaatt ccgctttcgt tatgaacaat
     5641 aatttacgta gggtacgacc atgaccagcc gttttatgct gattttcgcc gccattagcg
     5701 gcttcatttt tgtggctctg ggcgcttttg gcgcgcatgt gttaagtaaa accatggggg
     5761 ccgttgagat gggctggatc cagaccggcc tcgaatacca ggcgtttcat acgctggcga
     5821 tcttaggtct ggcggtggca atgcagcgtc gcatcagtat ctggttttac tggagtagcg
     5881 ttttcctcgc gttaggcacg gtgctgttca gcggcagcct ttattgcctg gcactgtccc
     5941 atctgcgttt gtgggcgttt gtcactccgg ttggcggcgt gagcttcctt gcgggctggg
     6001 cgttaatgtt agttggtgct atccgtttaa agcgcaaggg cgtaagtcat gaataaggtt
     6061 gtattgctgt gccgtccggg ctttgaaaaa gagtgcgccg cagaaattac cgataaagcc
     6121 ggccagcggg aaattttcgg ttttgcccgc gtgaaagaga atgcgggtta tgtcatttat
     6181 gaatgttatc aacctgatga tggcgataag ttaatccgtg agctgccgtt cagttcatta
     6241 atttttgccc gccagtggtt tgtggtgggg gaactcctgc agcatttgcc gccagaagat
     6301 cgtattaccc ccattgtcgg catgttacag ggcgtagtag agaagggcgg tgaactgcgt
     6361 gttgaagttg ccgataccaa cgaaagcaaa gagttgctga aattctgccg taaattcacc
     6421 gtgccgctac gcgctgcctt gcgcgatgcc ggggtgctgg cgaactatga aacgccgaag
     6481 cgtccggttg tgcatgtatt cttcattgca ccaggttgct gctataccgg ttactcatac
     6541 agcaacaata attcgccgtt ctatatgggc attccgcgcc tgaaatttcc ggcagatgcg
     6601 ccgagtcgtt ccacactcaa actggaagag gcatttcatg tgtttattcc tgcggatgag
     6661 tgggatgaac gcctggcgaa cgggatgtgg gcggtggatt tgggcgcttg cccaggcggc
     6721 tggacctacc aactggtgaa gcgcaacatg tgggtttatt ccgtcgacaa cggcccgatg
     6781 gcgcaaagtc tgatggatac cgggcaggtg acgtggctgc gggaagacgg tttcaaattc
     6841 cgtccgacac gcagcaatat ctcctggatg gtatgcgata tggttgaaaa accggctaaa
     6901 gttgcggcat tgatggcgca gtggctggtt aatggctggt gccgtgaaac cattttcaat
     6961 ctcaaactgc cgatgaaaaa acgctacgaa gaagtatcac acaatctggc gtatattcag
     7021 gcacagcttg atgaacatgg cataaatgct cagattcagg cgcggcagtt gtatcacgat
     7081 cgcgaagaag tgacggtgca cgtccgccgc atctgggctg cggtgggtgg tcgtcgcgac
     7141 gagcgataac aggcaaggcc ggggcgtcgt ctccggcctg ctaccctttc cgtcaggctg
     7201 ttaccaaaga agttgcaacc tggcgctcat cagaaataat gtgcgttacc tcatccagat
     7261 gcccgatgcg ggcttcccct gaacgattaa atttactttt atcaatcaat aacaacgatt
     7321 gcgcggcacg ttttaatagc atcgatttgt aatcagcgtt gatggcattg gagtcccaca
     7381 gtgcgccgct gctatcgatc ccttcacaag aaaaaataaa cagatcgatt tccagcgatt
     7441 taagttggga aatcagcgag ggattgacgt aacagccata tttgcgctca agtgtgccgc
     7501 cggaactgat cagttgaatg cgttcgcgtt taccgagttc atggcaaatc ggatggctat
     7561 tggtgaagac ctgaatgttg atgtcaggca actggcgtgc cagataccag caagttgaac
     7621 tggcgtctaa ggctatcacc atcccttctt caatccacgc gagcgcctcg cgcgcgatat
     7681 ctgctttatg cgcataatgg cttttcagcc ggatgtgaaa gggatcgcca ctgtcttgat
     7741 tttgacggtg gatatattta gcgcgtccat gattgcgcag aattttaccc tgcgtctgta
     7801 attcattgag atcgcgacga atggtttctt tactgacctt tagctgttca gagagagctt
     7861 ccgtggtcag gctggtatgg ttcagcagca ggtcgactat cgcttgctgg cgtgccgctt
     7921 tcatattttt caccccatca ttcgataacc gcccgcgtca tgcgggcgta ccggtatgag
     7981 attacggtgt tacccctttt tttaaaagaa tattcccgta cttcgctcgt tcgcctgtga
     8041 taacgatcgc aaaggctttt tgcgcccgtt cataaaacgc aaaacgattg atgcggatga
     8101 tgtcaggaca cggggcttgt agtgaaagcg cattacggta acgtcgttct acttcaggat
     8161 cgagagtgtc accttctacc gctgccatca tcaccagcgg cggggcataa ctgtccagct
     8221 caaataacgg gataatcgcc tggagcaagt cgctcaccaa caggccatca gcgcggatca
     8281 cctgcggtcc catcgaatgg gcgggaaagt gagcatcgga aaaaataatt tcatctccat
     8341 gtcccatctc tgccagcact ttcaatagtt cgggagaaat taacggcgaa attgttttca
     8401 gcatttcaca cttcctctat aaattcaggt tcagtttgcg ggtagaaata acggtactga
     8461 taatgaatct gtgcacgggc ttcttccggg ctgttgaatt cccctacgcc ataccaaccg
     8521 aacagcgcag ctcctgcgac ggtcgtttcg gcatcgtcga gaacttttac cggaatatca
     8581 agcatattgg ctttaatctg attccacaat gtgttgcgac ttcctccacc gactaacaat
     8641 aattcagagg ccttaaagtg cccgattttt tccagcatct gtagattgcg ctgtaattgc
     8701 gcagttaacc cttccagcgc cgcgcgatag aaatgccccc gcgtggtatt aagcgtcact
     8761 ccttgccagc cagcgttctg acacgacaat aaatcacact gcatttttac gccatccgcg
     8821 ccaggcgcga tcagacgagc ttcttcaatc aacatttgcc agggtgtttc agccgtccag
     8881 aacagttttc tcacccattc cagcacgccg gatgccagcc attgcatacc tgggttatac
     8941 aaccctgcct ggctatccag ttcgcaggtg gaaccggcgt actgacttaa cagcgaagta
     9001 tcaacctggg cgctacggac cattaaaatt tcccatgtac cggaagagag cacgggttca
     9061 ttttggtcgg caccagcgcc aaaaagggcg aactgggtat cgtgacctgc ggaaatcacc
     9121 ggtatgccaa cgggtaagcc gagcattgct gcggcgctgt tctgtagcgt accaatctgt
     9181 tcacccgctt ccaccagacg agggaagagt cggcgtggaa taccggtggc ttgtaaaatt
     9241 tgcggactga aatcgcgttg ctggatatcc agcatctggc tggttccggc catcgtgata
     9301 tcagtagtga attcgccggt taaacggtgg ttaatcagcg acgaaataaa gagccaggcg
     9361 tgcgcgcgtt ccagcagttg tggatgattt tctttcaacc acaccaactt atataacgta
     9421 ttgaaactaa aggctccgac gccagaaata gcctgcaacc gctgtgcgga gatcaactgt
     9481 tcaatattgt ccataaccgc tgctgttcgc ggacatttcc agctaatgat cggatagagc
     9541 agattgcctt gcttatctac cagagcgcca tccacaccaa aggtggtgac ggcgataccg
     9601 cggatgtggc attcagtcag ttcactattg atttgccgac agcaatcagc aaagcgttgc
     9661 aaaatggcgt ctaaagacca ctggtgccag gtgttgtttt ccatcgcgat atcgctggca
     9721 ttaggcgttg aggcgcgggc aacaattttt ccctgccgat taaccgcgat ggccctgaca
     9781 ttggtcgcgc cacagtcgag taccaggata acttcttgtt tcataatcgc tcctgcaata
     9841 tagccggata acattgctta tccggctaac cactcttgct tgttcaggcc cggtagccgg
     9901 agcgaccggg catcacatca gggagtaatg tattaacgct tgtacaacgg gccgtagttc
     9961 tggcaagcgc ggtaatcctg gccttcaata tccatgccgt gcgcagccca ggcagaagga
    10021 cgatacactt tggtctcttc aacgttgtgc atacataccg ggatacgcag catggaggcg
    10081 agagtgataa agtctgcgcc aacgtggccg atggtcagaa ccccatggtt agcgccccag
    10141 ttcgccatta ccgagtacac atccgtaaac ggccctttac cggtgaggcg cggtgcaaac
    10201 caggtggttg gccaggttga gttggtgcgt ttgttgagga tgtcatgcac atccttcggc
    10261 aattccacgc tccagccttc cgcgatttgc agtaccggtc ccaggccttt gatgatgttg
    10321 acacgggtca tggtgaacgg gacgccgcct tcggtaagga agcgggaaga gtaaccgccg
    10381 ccacggaagt attcgtggat cgccgggcac cattcggtag cggcgaggca agcgtcagcc
    10441 tcttgctgag agatttccca gtgcggcttc atcgtcgggt taccttcgct gtcgcgttgt
    10501 ttacaggaac cgtccagcgc agcagaaccg gagttgatca aatggatgat gccgtgttct
    10561 gccagtccat cgagtttatg ccccgttaca cgctcaattg cttctggtga ccagtaggta
    10621 cgcacatcgg caaatacctg agcggtgccg gtgagctggt gacccattag cattgccacg
    10681 ccgttaagac tgtcgttttc ggtcgccacg acaaagggtt cgcgcacgcc attccagtca
    10741 aatgaactgt taaggatcgc ttcggcggtg tcaccattgg gatattgatc ggtccagtga
    10801 cgttgcccct ggaagcccgc agcgatggcg ttgtagccaa gtgattcttc cacgcgacca
    10861 atatcggcca gtttgctgtt gccttgcatc atgtcgcgga tacacatcgc catcagtaaa
    10921 ctttcgcgca gaactgcgcg gctttgctcg gcattgcgtt gatactgttt gttattttca
    10981 tcttcgccat agcggaagtt tttatcagcc caggtcagtg ccatttccag ttcggcttcg
    11041 tcgtaaatct tctgatcgat acggcgacgc agttcggtca tatccaccgc ctggactttc
    11101 attcccagcc aggattcaaa gaagttgtga tcaacaatag aaccggcgat acccatcgaa
    11161 acgccgccca gcgacagata gcttttacct ttcatgctgg cgacggccaa accggcgcgg
    11221 gcaaagcgca gcagtttttc ttcaacatcg gcaggaatcg atgtgtcatc ggcatcctga
    11281 acgtcatgac cgtaaatgga gaatgccggg atgcctttct ggctgtgagc tgccagagcc
    11341 gctgccaggt aaacagcgcc gggacgttca gtgccgttaa agccccaaat ggctttcggg
    11401 cgggttggat ccatgtcgat ggtttcactg ccatagcacc agcaaggcgt taccgtaatg
    11461 gtgaggccta cattctgact gctgaatttt tcttcgcaag cagcggcttc agccataccc
    11521 gcgatacagg tatcggaaat gacacactcg acggcagctc cgcaggcatg gcgcagtttc
    11581 tcggtcagca gtgcggccgt agctttcgcc atattcattg tttgttcttc aagcgactca
    11641 cgaacaccca tgcgacgacc gtcaataacc gggcggatac caattttcgg taagctgatt
    11701 tttttcatta cagattcctc actttattcg gaaaatatgt tcagttagtt gccgtttgag
    11761 agcggaaacg ggcaaagata aagatgaccg cgaagcagag tgcggggatc agttcagcag
    11821 tggggatgtt gcccgccgcg tcactgacaa aacccatgac cggagtgaca ataccgccgc
    11881 caatgatggt cataacgatg aaggacgaac cgtatttggt gtcctggccg agattcttaa
    11941 tgcccagcga gaagattgtt gggtactgaa tcgacataaa ggcgctgcat aaagtcaggg
    12001 ctattaagcc cacatgaccg ccagcgaagg ctgagatcag gcacagtgcc atagcgatta
    12061 atgcgtaggc ggccaggact ttgtgtggtg cgaagcgact gatgagccag gtaccggtga
    12121 aacgaccaat aaagaagcac accatggttc cggttaaata gttagcggca aagcctgcag
    12181 tcatacctgg aatttcttct acagcgtagc gaatcaaata gctccagcag gccgtttgtg
    12241 cgccaacgta gcagaattgc gctaataccg cccagcgcca gtgacgaata cgcgccagac
    12301 gagaaagcga tgcggagaac gatccttgtt tggcgtcact gtgattatca ctctgcaatg
    12361 ccgggaattt cgtcagcatg atcagcaggg cgaccagtaa cacgatagcc acgatgatca
    12421 tataaggtgt ctgtaccgat aataccaggc tgtgtttata cgcactcaat tgctctggag
    12481 acattttatc gagaacgtct tgcgattgat gtggcacgtt agacaaaata agactttgcc
    12541 caaagacaac cgcgataatt gcgccaaacg agttaaatgt ttgcgcaaga tttaagcgga
    12601 agtgaccact gctttccggc cctaataccg taacaaaagg gtttgcggca gtttccagac
    12661 aacctaatcc ggctgcaata ataaataggc caactaaaaa taaggtgtag ttcattattt
    12721 ctgcagcggg ccagaataat gcagcgccca aggcatataa aaataatccg gtaataatcc
    12781 ctgctttata actgagtttt ttcatcaata tcccagcagg gattgggata atgaaataac
    12841 caaagtaaaa ggccgattgg atcaggccag cctggaaatt tgtcagcgta aaagcctgct
    12901 ggaattgagg taataaaatg tcgttaaggt tattggctac cgcccaaaga aaaaacagtg
    12961 agcacagcag cgcgaatgga ataatgtaac ttctgctttg ccctgcatct ttatctaccg
    13021 cacggtaact ctgcgtttgt attgatgtgt ttcccatagc atcctcttag gttcagaagc
    13081 ttaagcacgt aatcggttat gttttcaaaa gtgcaaaacc agcgagttcg ggcttcatat
    13141 cagtgaatat ttgcctgtct gaaatgaact catggattca ttttcaaata aaaaacgctc
    13201 tgtaatatga cggcggtcac acttattagc tggaaggtga ctttatgtga ctaccatcac
    13261 tttaataatc gaaatttcat gcccgtttca gaaaataaat tataaaaaac acaaaccaga
    13321 tgaccgtatt aattgtttat gccgaaaacg ggcattcctg gcttgagtga atagaaaaat
    13381 gaccgttttg ataaatattt ttaagagcgg tcatttttaa tggggttata aaaagagaac
    13441 ttgcagttat ttactgtgat tagttgaacc aggtcacaaa atataagctt gtgaatttct
    13501 ttctggttaa tcaggaagaa gatggtgggt aattaaacgg ctaattcaat agtgtgaaag
    13561 gaacaacatt attgccctgt tttgaatcag agagaggtag ctaatggaac gaaataaact
    13621 tgctcgtcag attattgaca cttgcctgga aatgacccgc ctgggactga accaggggac
    13681 agcggggaac gtcagtgtac gttatcagga tgggatgctg attacgccta caggcattcc
    13741 atatgaaaaa ctgacggagt cgcatattgt ctttattgat ggcaacggta aacatgagga
    13801 aggaaagctc ccctcaagcg aatggcgttt ccatatggcg gcctatcaaa gcagaccgga
    13861 tgccaacgcg gttgttcaca atcatgccgt tcattgcacg gcagtttcca ttcttaaccg
    13921 atcgatcccc gctattcact acatgattgc ggcggctggc ggtaattcta ttccttgcgc
    13981 gccttatgcg acctttggaa cacgtgaact ttctgaacat gttgcgcagg ctctcaaaaa
    14041 tcgtaaggca actttgttac aacatcatgg gcttatcgct tgtgaggtga atctggaaaa
    14101 agcgttatgg ctggcgcatg aagttgaagt gctggcgcaa ctttacctga cgaccctggc
    14161 gattacggac ccggtgccag tgctgagcga tgaagagatt gccgtagtgc tggagaaatt
    14221 caaaacctat gggttacgaa ttgaagagta atttcgtaaa gcaacaagga gaaggatgat
    14281 ggctaacaga atgattctga acgaaacggc atggtttggt cggggtgctg ttggggcttt
    14341 aaccgatgag gtgaaacgcc gtggttatca gaaggcgctg atcgtcaccg ataaaacgct
    14401 ggtgcaatgc ggcgtggtgg cgaaagtgac cgataagatg gatgctgcag ggctggcatg
    14461 ggcgatttac gacggcgtag tgcccaaccc aacaattact gtcgtcaaag aagggctcgg
    14521 tgtattccag aatagcggcg cggattacct gatcgctatt ggtggtggtt ctccacagga
    14581 tacttgtaaa gcgattggca ttatcagcaa caacccggag tttgccgatg tgcgtagcct
    14641 ggaaggactt tccccgacca ataaacccag tgtaccgatt ctggcaatcc ccaccacagc
    14701 aggcactgcg gcagaagtga ccattaacta cgtgatcact gacgaagaaa aacggcgcaa
    14761 gtttgtttgc gttgatccgc atgatatccc gcaggtggcg tttattgacg ctgacatgat
    14821 ggatggtatg cctccagcgc tgaaagctgc gacgggtgtc gatgcgctca ctcatgctat
    14881 tgaggggtat attacccgtg gcgcgtgggc gctaaccgat gcactgcaca ttaaagcgat
    14941 tgaaatcatt gctggggcgc tgcgaggatc ggttgctggt gataaggatg ccggagaaga
    15001 aatggcgctc gggcagtatg ttgcgggtat gggcttctcg aatgttgggt tagggttggt
    15061 gcatggtatg gcgcatccac tgggcgcgtt ttataacact ccacacggtg ttgcaaacgc
    15121 catcctgcta ccgcatgtca tgcgctataa cgctgacttt accggtgaga agtaccgcga
    15181 tatcgcgcgc gttatgggcg tgaaagtgga aggtatgagc ctggaagagg cgcgtaatgc
    15241 cgctgttgaa gcggtgtttg ctctcaaccg tgatgtcggt attccgccac atttgcgtga
    15301 tgttggggta cgcaaggaag acattccggc actggcgcag gcggcactgg atgatgtttg
    15361 taccggtggc aacccgcgtg aagcaacgct tgaggatatt gtagagcttt accataccgc
    15421 ctggtaaatg cgctgatgtg ataatgccgg atacgacgtt tgcggcgtat ccggctcgcc
    15481 gttaccgtac caaccgcaat tgctgtaaat tgccgtcgat atgcaaatcg gtttgtaagc
    15541 gggcaatatc gcggcacaga aacgccatct ctttatgggt ttctaatttt ttgcgccact
    15601 tttcggcaac cgcatccaga ttctcatata tcccttccag actctgaaac tcgaccagca
    15661 gctgcgtggc gctttttggt ccgattcccg caacacccgg tacctttgaa ctgctgatcc
    15721 ccgcaagtcc ccagtaatcg ggcaactgct gcggttgaac gccaaattct ttatcgataa
    15781 atggcgcatc cagccaacgt ttctggaagt aatcacgaat acgtaatgtc ggtgaaagta
    15841 actgacagta gcctttatct gtcgaaacaa tcgttgcctg atgcccggcc tgtgtcactt
    15901 tgaccgccag cgtggcggct aagtcatcgg cttcgttgcc gctggttgac cagcacggga
    15961 cgccgcgttg ctcaaaggcg gcgcgtaatg caggcatctc gtcgtgcaac tcttccggca
    16021 ttggcggtcg acccgctttg taatctggta aacgctgatg acgccagccg ctactgcggt
    16081 tttcatcatc aaaaacggcg accgcgtggg ttggctggct gtgcataatg agctgatcga
    16141 gcgcatgctg acaggtttcg acacagggcg atccctgaac ggcatgaatg cgacgaataa
    16201 gattcagtgc atcgacaata agcaaatgaa cagccacgat aaccccctta aagatcaggc
    16261 gataagggta acattcgatg ccggaacagg ctacggctgg atgagaaatc gggaagaggc
    16321 ctcgcaaaac gaggcctctg gagagcgatt aatcgcaggc aacgatcttc attgccaggc
    16381 cgccgcgaga ggtttcgcgg tacttggcgt tcatatcttt acctgtttcg tacatggttt
    16441 cgataacttt atcgaggcag acgcgcggct cgctggtacg gcgtagcgcc atacgtgcgg
    16501 cgttcaccgc ttttactgcc gcaatggcgt tacgctcgat gcatggcacc tggacctgtc
    16561 cggcgaccgg gtcacacgtc agaccgaggt tgtgctccat ggcgatttcc gccgcgatgc
    16621 acacctgcgc cgggcttgca cctaacagtt ctgccagacc agccgccgcc attgagcacg
    16681 ccacaccaac ttcaccctgg cagccgactt cagcaccaga aattgacgcg ttcatcttat
    16741 aaagagaacc aatggcactg gctaccagca ggtaacgagc cagtgagtta gcgttcactt
    16801 cgcggataaa cttgtcgtag tacgccagca ctgccgggat aatcccgcac gcaccgttag
    16861 tcggcgcagt caccacgcgt ccgccagcag cgttctcttc gttcactgcc agtgcaaaca
    16921 tgttgatcca gtcaacaacc gccatcgggt cagtggtggt tttatcctgg ctgaccagca
    16981 tccggcgtag tgccgcagca cggcgtggaa cgcgcagttt gccaggcaac acgccttcgg
    17041 tggaaatacc gcgctcaata ccgccacgca tgacttccca tacgttcgcc aggtgctgtt
    17101 ccagctcttc tttgctgtgc agcgccagct catttttcat catcaggcca gagagtgaca
    17161 gcccggtttc ctgacaatgt ttttgcagat cggctgctga actgtacgga taaggaactt
    17221 caaccggtgc gctattctgc tggccaaaat gctcttcatc aacgataaag ccaccgccaa
    17281 tagagtagta agtctggctg taaacgactt tatcgcccgc cagcgcggta atgcgcatac
    17341 cgttttcatg cagagaaagg ttgtctgcat ggaagttcat gcactgatca accgggaact
    17401 ccacttcatg ctgaccgttt gccagcatca ggcgaccatg agtattcaca tcctgaataa
    17461 aaccagggat ggaatcgata tccacggtat ccggcaggtt acccgccagg cccataataa
    17521 tggcgatatc agtgtggtgg cctttaccgg tcagagagag cgagccgtac acgtcaacca
    17581 ccacgcgggt cacgtcttta agcagattac gggcaatcag atcgtcggta aattgtttac
    17641 ccgctttcat tggtccaacg gtatgagaac tggaagggcc aatgccgatt ttgaaaatat
    17701 cgaatacgct aatcatagga aatacatcgc gttaaaacgg aggaagcgcc gcccgaaagc
    17761 ggcgcgaaag gacttagctg aacagagagt agaagattgc ggagattgca atcagaccca
    17821 tcacgacaac gaatacgttg ctgatgtgac cgctgtactt acgcattgcc ggtactttct
    17881 gaattgcgta catcggcatc aggaacagga tcatcgcgat gattgggccg cccagggttt
    17941 caatcatacc caggatgctc gggttcaggg tggcaacaat ccaggtcgtt accagcatga
    18001 acagcgcagt gatacggttc agcttgttga tttcgataga cttaccttta ccacgcagag
    18061 atttaatcac cataccgttg aagccttcac gtgcgcccag gtagtggccg aggaaggatt
    18121 tggtgatagc gataatcgcg ataatcggag ccatccacgc gataaccggt gcgttaaagt
    18181 ggttagccag gtaagacaga atcgagatgt tctgctcttt agccgcagcc aggtctgccg
    18241 gagtcaggct cagtacgcag ctgaagacga agaacattac ggtcagcacc atcatgatgt
    18301 gtgcgaatgc aaggatcttc gagcatttct gttctgccat atcgccgtac tcttcacgct
    18361 tcgcaacggc gaaagaagag atgatcggag agtggttgaa cgagaacacc attaccggaa
    18421 ttgccagcca cagggtcatc cacagaccgt tcccggttgc agatgcagtg tccagagaca
    18481 gcgtttccag tgctgcgccg ttccactgcg ggatcaggta cagagccagc agcatcagta
    18541 cgccaacaaa cgggaatacc agaatactca tcgctttaac gatcatctgc tcaccaaagc
    18601 gaacgatggt catcataccc acgatcagga tcagcgacag aatcgcacgc ggcggtggcg
    18661 tcatacccag ctggtgagac atgaagcttt caactgtatt ggtgattgcc acgctataaa
    18721 ccagcaggat cgggtagata gcgaagaagt agagcagggt aatcagttta cctgcgccaa
    18781 taccaaagtg ttcttctaca acctcggtga tgtcttcgcc cgggttttta ccagacagta
    18841 cgaagcgagt caggccgcgg tgagcaaaaa acgtcatcgg gaacgcaagg atagccatga
    18901 tgatcagcgg gatcataccg ccaacaccgg cgttgattgg caggaacagc acgcccgcgc
    18961 cgattgccgt gccgtaaagg cccagcatcc acatggtgtc tgtcttgcgc caggcactac
    19021 gagagtcttt cgacgcaatc gtgctggttt gagtcgtttc catctatttc tcctggagga
    19081 atgcaaataa ttcagctttt agccagttca gcccgggaag gtgcctgacc gtaaaaatga
    19141 aatctgtcta tcggcgatgg cttaatcatt taccgccgta ataatttgag ggcggaaaga
    19201 tacacacaag ttatgtatct atcagtgatc ttgatctcaa atacaataat ctacttggaa
    19261 gatagattat cccaacgctt tactctgtaa atcaaaagta aaagagaatt taagggcaat
    19321 atttgcccat taaacgacat ctgacttctt ccggggcgcg caatatatgt aggttgaact
    19381 gaaaaaatca atacttttag ttaatcatgg cgataatttc tttttcatag cgataaaata
    19441 cggtttatga aaatagggaa acgtttgcgt tgcaacataa attgtatgac atattaactg
    19501 tgatgatatt tgtcgcgtga gggtggtaag aaacattcat ataacaaatg aaatgagttg
    19561 atgagtaagt gaccaaaaac gagaaaaccc ggcaacaaac gtcaccgggg gagcggagat
    19621 tacgtgcaga tttcgtagca agggatgtag gctgagcctg gcaacttcat acgatgctgg
    19681 gcaacaaaac cctgtagcag gtcgtccata cgacgcataa tctctttatc gccgttgatt
    19741 ttgtaaggac caaactcttc aatggcgcga ataccgactt cttttacgtt acccgccaca
    19801 ataccggaga acgcacggcg caggtcggca gccagcactt caacaggttg atccgggtaa
    19861 agcttcagat tagccatatt ctcgtgagac ggctcaaacg gcatttgcaa atctggtgca
    19921 atgcgcattg accagttaaa gctgtaagcg tcaccagtgt cgcggcgatt ttctttcacc
    19981 agcggcatcg attttttcat ctgacgagcg acttcagcgg cgtcatcaat gatgatgcgg
    20041 taatggcggc gcgcgttttc gcccagcgta tgtacgacaa actcgtccag tacgcggaag
    20101 tagtcggcgc tctctttcgg gccggtgagg atcaatggta aaacctgatc tttgttggcc
    20161 gggttcatta aaattcccag caaatacagc aactcttctg ccgtacccac accgccaggg
    20221 aagataatga tgccgtgagc gatacggaca aacgcttcca gacgtttttc gatatccggc
    20281 atgatgatca attcgttgac cagcgggtta ggcggttcag cggcgataat cgacggctct
    20341 gtcataccaa taaaacgact gtctttgtaa cgctgctgcg cgtgtccgac cgcagcacct
    20401 ttcatcggcg cttccatcgc tcccggacca cagccggtgc agatattcag ctcacgcagg
    20461 cccagctggt ttccgacgcg acgggcatac aaatactcat tttcgttaat tgagtgaccg
    20521 ccccagcaga ccaccatatt tggcgcttca cccacatgca gcgcgcgagc gttacgcaag
    20581 atggaaaaga ccaggttagt gatgtggacg gagttgtcga gattcaggtt gggaaaacga
    20641 acggtgttat ggatttgccc gtaaacgaag agaatgtcac gcaggaccgc gaacaagttg
    20701 gcctgcaaag cgcgaataat tcgcccatcg acaaaagcct cttccggggg attaatcagt
    20761 tccagcttta cgccgcgttc acggcgcaag acgttaatat cgaaattttc aaaacgagac
    20821 agcaattctt tgctgttatc ggtcaaacta ccggagttca gtacggcaag tgaacagttg
    20881 cgaaacagtt gatagaggtc gctgctggcg gtgcgtttaa gcatatccac ttccagctgc
    20941 gacaacatat ccatggagcc aagcgggcta atatgtgtaa tcaagaaaaa ctccttatgg
    21001 gacgaatatc gtcattccct ttgattacaa tagccctgcc tgatgacctt tcacaacctt
    21061 ctgcgtgaat ccagcacgca aaattgagaa aaaatttatt gccgaaccag tcttgtggtc
    21121 gatgggacaa aatcgctatt actgcgccac gggttaatgt ccagaccgcc acgacgggta
    21181 taacgtgcgt aaacgctcaa tttttctggc tggcagaagc gtaacaggtc attaaagatg
    21241 cgttccacgc actgttcgtg gaactcgttg tgatgacgga atgagaccag gtaacgcagc
    21301 agtttttctc tgtcaatttg gcgtccacga tactgaattt ggatcgaacc ccaatctggt
    21361 tgatgggtga tcaggcagtt tgatttcagc aggtggctga caagcgtctc ttccactact
    21421 ttttcaccgc tggtggcatt ctccagatag tcagtagtga attcatagtt atcgatagtg
    21481 atatcctggt catcaatgca ggtgccattg aaatgaccta tcggctggcc ttccagttca
    21541 tcaagacgat ataacgccac gctaacctta ccctgggcgc aagtgcttaa gtcgcgctcc
    21601 agcgtctggc gcacctcatc ccagttatta aaacgcgtct ggttaaaact gttgagatag
    21661 agtttaaaac tcttcgactc aatcagattt acgctggtgt aatcaagttc aacatgaccg
    21721 acagcgacct gcggcaaacc tttcgcattc agccaggaaa gttcatacag cgtccagata
    21781 tccgtaccgt gaaaaggcag gttatccgct ttcagaccca gcgggtcgcg attcaggctg
    21841 cgtggaacgc cttgcagtag gctggcgtca taggtatccc ggtaatcggt tgattttcca
    21901 agagtcaggc ccgcaagtgc ctgatggttt gcataagaag acatgtttca ccgtcataaa
    21961 ccagataaac taaacgacaa gtgtatctgg tttaagtgaa gagagaaatt agtggacgat
    22021 ttgaccgcac aagccctgaa agattttact gcacgctact gcgatgcgtg gcatgaagag
    22081 cataaaagct ggccgttaag tgaggaactg tacggtgttc cttcaccgtg cattatttca
    22141 actaccgaag atgccgtata ctggcaaccc cagccgttta ccggggaaca aaatgtaaac
    22201 gcggttgaac gcgcttttga tattgtgata caacccacaa ttcatacctt ttatacgact
    22261 cagtttgccg gggatatgca cgcgcagttt ggcgatatca aactgacatt gctgcaaacc
    22321 tggagtgaag acgacttccg tcgggtgcag gaaaatctga ttggtcatct ggtgactcag
    22381 aaaaggctaa aactgccgcc cacgctattt atcgcgacgc tggaagaaga gttagaggtc
    22441 atctcggtgt gtaatctctc cggtgaagtg tgcaaagaga cgctgggaac acgtaaacgg
    22501 acgcatttag cctcaaatct tgcggaattc cttaatcaac ttaagcctct tctgtaatcc
    22561 aaatacccca tctggttgtg agagatctct tacagactct gtaggagatc gccaggaaat
    22621 aagcgaatac ttaaaaagat aagaatcgtt attttcattt aaaatcaaaa tgttgattgt
    22681 taattcttaa ctttcatatg aaattttcct taaggcattg tctgtaagcg ccttgtaaga
    22741 cttcgcgaaa aagacgattc tatcttcgtc gacagggagt cagacaacga agtgaacatc
    22801 aggatgatga cacttctgca ggacacacca ggatggtgtt tcagggaaag gcttctggat
    22861 gaagcgaaga ggatgacgca ggacgcgtta aaggacacct ccaggatgga gaatgagaac
    22921 cggtcaggat gattcggtgg gtcaggaagg ccagggacac ttcaggatga agtatcacat
    22981 cggggtggtg tgagcaggaa gcaatagttc aggatgaacg attggccgca aggccagagg
    23041 aaaagttgtc aaggatgagc agggagcaac aaaagtagct ggaatgctgc gaaacgaacc
    23101 gggagcgctg tgaatacagt gctccctttt tttattcctg ctatccttcg cggcagtttt
    23161 tcttattgag gttgctttat gaccactcat gaccgcgttc gtctccagtt gcaggcgctt
    23221 gaagcgttac tgcgtgaaca tcagcactgg cgaaacgatg aaccccagcc gcatcaattt
    23281 aatagtaccc aaccgttctt tatggacacc atggaaccac tggagtggtt gcagtgggtg
    23341 ctgatcccgc gaatgcacga tttgttagat aacaaacagc cgttaccggg cgcttttgcc
    23401 gttgcaccct attatgaaat ggcgctggcg acggaccatc cgcaacgcgc gcttattctg
    23461 gctgagctgg aaaaactgga tgctcttttt gcggatgatg cgagctaatg ctggaaatac
    23521 tctatcagga tgaatggctg gttgcggtaa ataaaccctc cggctggctg gttcaccgca
    23581 gctggctgga tcgcgacgag aaagtagtgg tcatgcaaac cgtgcgtgat cagattggcc
    23641 agcacgtttt taccgctcat cgtctggacc gacccacttc tggtgtgttg ttgatgggat
    23701 tatccagcga ggccggacgg ctgctggcac aacagtttga acagcaccaa atccagaaac
    23761 gttaccatgc gattgtgcgc ggctggttga tggaagaagc agttctggat tatccactgg
    23821 tggaagaact ggacaaaatc gctgataaat ttgcccgcga agataaaggc tcgcagccag
    23881 cagtgacgca ttatcgcggt ctggcgaccg tagaaatgcc ggtagcgacc ggacgttacc
    23941 cgaccacgcg ctacggcctg gtggaactgg agccgaaaac cggacgcaaa catcagcttc
    24001 gccgacatct tgcccatttg cgtcatccga ttattggcga tagcaaacat ggcgatttac
    24061 gccagaatcg cagcggtgct gagcattttg gcctccagcg gttaatgcta catgccagtc
    24121 agttgtcatt gacgcatcct tttactggcg agccgctgac tattcacgcg ggcctggacg
    24181 acacctggat gcaggcgtta tcacaatttg gctggcgcgg tctgcttcct gaaaatgaaa
    24241 gggttgagtt cagtgcgcca tcgggtcagg atggagagat aagttcctaa tccagggaga
    24301 aaacggtatg gcggaaattg gtatttttgt cggcaccatg tacggaaatt cgctgttagt
    24361 ggcggaagaa gcagaagcaa ttctgaccgc acagggccac aaagcaacgg tatttgaaga
    24421 tcctgaatta agcgactggc tgccctatca ggataagtat gttctggtgg ttacgtccac
    24481 gaccgggcag ggcgaccttc ctgatagcat tgtgccgctc tttcagggaa tcaaagatag
    24541 tctgggcttc cagccgaatc tgcgttatgg cgtgattgcg ctcggcgaca gcagttatgt
    24601 aaatttctgc aatggcggca aacagttcga tgccttgttg caggaacaga gcgctcagcg
    24661 ggttggcgaa atgctgttga ttgacgccag tgagaacccg gaaccggaaa cggaatcaaa
    24721 tccgtgggtc gaacagtggg gcacgctgtt gtcctgatat gttcagcgag cggtaaatgt
    24781 cgttttagcg gtgctgaatc gaatattttc aggcaaatgc cagtaaaaac tgcttcatag
    24841 cgcggatttt tactggcgtt tgcctggagt caagcgatcc atttcatact cttctttatt
    24901 tcttcgtttt aacccttcct ttcttgttct tgttttcatt tccgtgaagt ggattccacc
    24961 gtccagggct aatgccaaaa tcgggcctca ttgaacgcat taatgttgtg ttgttgcacg
    25021 gtgagccgct atggcgcgct ttttatactg ctattgccag atataaacac gcgccgtatt
    25081 cggcgaacga cctataaaaa cggcaaaaaa caccctacgt cacctctgat ttcctggcta
    25141 tgtcgcagtc cagagtgagc gtggctaacg cgaattttca ggagtgcaac aatgagttct
    25201 ttaagtcagg ctgcgagcag tgtggaaaaa cgcacgaatg ctcgttactg gatagtggtg
    25261 atgttgttta tcgtcacatc cttcaactac ggcgaccgcg ctacgctttc tattgccggt
    25321 tcggaaatgg ccaaagatat cggccttgat cccgtgggaa tgggctatgt gttctctgcc
    25381 ttctcatggg cttatgttat cgggcagatc cctggtggtt ggttgctgga tcgttttggt
    25441 tcaaaacgcg tctacttctg gtcgatcttt atctggtcga tgtttacctt gctgcaaggc
    25501 ttcgtcgata tctttagtgg attcggcatt atcgttgccc tgtttactct gcgcttcctg
    25561 gtcgggcttg ctgaagcgcc atctttcccc ggcaacagtc gcattgttgc ggcctggttt
    25621 ccggcgcagg aaaggggaac ggcggtgtcg atttttaact ccgctcaata cttcgcaacg
    25681 gtgatcttcg cgccgattat gggctggctg acgcatgaag tgggctggtc acacgtcttc
    25741 ttctttatgg gcggtctggg gattgtcatc agctttatct ggttgaaagt catccacgag
    25801 ccaaatcaac atccgggggt aaatcagaaa gagctggagt acatcgccgc gggtggcgcg
    25861 ctgatcaata tggatcagca aaacaccaaa gttaaagtgc cgttcagcgt gaagtggggg
    25921 cagatcaaac aactgctcgg gtcacggatg atgatcggcg tttatatcgg tcagtactgt
    25981 atcaacgccc tgacttactt tttcattacc tggtttccgg tttatctggt gcaggcgcgt
    26041 gggatgtcga ttctgaaagc gggctttgtg gcttccgttc cggcggtttg cggttttatc
    26101 ggcggtgtgc tgggtgggat tatttccgac tggctgatgc gccgcacggg atcgctgaac
    26161 attgcgcgta aaacaccgat cgtaatgggc atgttgctgt cgatggtgat ggtgttctgc
    26221 aactacgtca acgttgagtg gatgatcatc ggctttatgg cgctggcctt cttcggtaag
    26281 ggcatcgggg cgctgggttg ggcagtaatg gcagataccg cgccaaaaga gatcagcggt
    26341 ctttccggtg gcctgttcaa catgttcggt aacatttctg gcatcgtcac gccaatcgca
    26401 attggttata tcgttggcac gactggctcc tttaatgggg cgctgattta tgttggtgtt
    26461 catgccttaa tcgcggtact gagctacctg gtgctggtgg gcgatatcaa gcgtatcgag
    26521 ctgaaacctg tcgcggggca ataagatgac gacacaatcc agtcctgtta ttactgatat
    26581 gaaagtcatt ccggtcgccg ggcacgatag catgttgctt aatattggtg gggcacataa
    26641 cgcatatttc acccgcaata ttgtggtact caccgataac gccgggcata ccggcattgg
    26701 tgaagcgccg ggcggagagg tgatttatca aacactggtc gatgctattc cgatggttct
    26761 gggccaggaa gttgcgcgcc tgaataaagt ggttcagcag gtgcataaag gtaatcaggc
    26821 agccgatttt gataccttcg gcaaaggtgc ctggactttt gaattgcgcg ttaatgccgt
    26881 ggcggcgctg gaagccgcct tgcttgacct gctaggtaag gcgctgaatg ttccggtctg
    26941 cgaactgttg gggccaggca agcaacgcga gactattacc gtcctcggtt atctgtttta
    27001 tatcggtgat cggaccaaaa ccgatcttcc ttatctggaa aatacgccgg gcaaccatga
    27061 gtggtatcag ttgcgccatc agaaagcgat gaacagcgaa gccgttgtgc gtctggcgga
    27121 agcctcacag gatcgctatg gctttaaaga tttcaaactt aagggcgggg tgttacctgg
    27181 cgagcaagaa atcgacactg ttcgtgcatt gaagaaacgc ttccctgatg cgcggattac
    27241 cgttgatccc aacggtgcct ggctacttga tgaagccatt tctctatgca aagggctgaa
    27301 tgatgttctt acctatgccg aagatccatg cggcgcagaa cagggtttct ccggtcgtga
    27361 agttatggcg gagtttcggc gggcgaccgg cttgcccgtc gcgactaaca tgatcgccac
    27421 caactggcgc gaaatgggtc atgcggtgat gctcaatgcg gtagatattc cacttgccga
    27481 tccgcacttc tggacgcttt ccggtgcagt ccgtgtggcg cagctttgcg acgactgggg
    27541 gctgacctgg ggctgccatt ctaataacca tttcgatatc tctctggcga tgtttaccca
    27601 tgtgggcgcg gcggcaccgg gtaatcctac cgctatcgat acccactgga tttggcagga
    27661 gggcgattgt cgcctgacca aaaatccgct ggagattaaa aacggaaaaa ttgccgttcc
    27721 tgatgcgccc ggtctgggcg tggaactgga ctgggaacag gtacaaaagg cacatgaggc
    27781 ctataaacgt ctgcctgtcg gtgcgcgtaa cgacgcaggt ccgatgcagt acctgatccc
    27841 cggctggacc tttgaccgta aacgtcccgt tttcggccgt cattgattct gaaaaaggac
    27901 ataaatatga gttctcaatt tacgacgcct gttgttactg aaatgcaggt catcccggtg
    27961 gcgggtcatg acagtatgct gatgaatctg agtggtgcac acgcaccgtt ctttacgcgt
    28021 aatattgtga ttatcaaaga taattctggt cacactggcg taggggaaat tcccggcggc
    28081 gagaaaatcc gtaaaacgct ggaagatgcg attccgctgg tggtaggtaa aacgctgggt
    28141 gaatacaaaa acgttctgac gctggtgcgt aatacttttg ccgatcgtga tgctggtggg
    28201 cgcggtttgc agacatttga tttgcgtacc actattcatg tagttaccgg gatagaagcg
    28261 gcaatgctgg atctgctggg gcagcatctg ggggtaaacg tggcatccct gctgggcgat
    28321 ggtcaacagc gtagcgaagt cgaaatgctc ggttatctgt tcttcgtcgg tgatcgcaaa
    28381 gccacaccgc tgccgtatca aagccagccg gatgactcat gcgactggta tcgcctgcgt
    28441 catgaagaag cgatgacgcc ggatgcggtg gtgcgcctgg cggaagcggc gtatgaaaaa
    28501 tatggcttca acgatttcaa actgaaaggc ggtgtactgg ccggggaaga agaggccgag
    28561 tctattgtgg cactggcgca acgcttcccg caggcgcgta ttacgctcga tcctaacggt
    28621 gcctggtcgc tgaacgaagc gattaaaatt ggtaaatacc tgaaagggtc gctggcttat
    28681 gcagaagatc cgtgtggtgc ggagcaaggt ttctccgggc gtgaagtgat ggcagagttc
    28741 cgtcgcgcaa ctggcctgcc gaccgcaacc aatatgatcg ccaccgactg gcggcagatg
    28801 ggccatacgc tctccctgca atccgttgat atcccgctgg cggatccgca tttctggacg
    28861 atgcaaggtt cggtacgtgt ggcgcaaatg tgccatgaat ttggcctgac ctggggttca
    28921 cactcaaaca accacttcga tatttccctg gcgatgttta cccatgttgc cgccgctgca
    28981 ccgggtaaaa ttactgctat tgatacgcac tggatctggc aggaaggtaa tcagcgcctg
    29041 accaaagaac cgtttgagat caaaggcggg ctggtacagg tgccagaaaa accggggctg
    29101 ggtgtagaaa tcgatatgga tcaagtgatg aaagcccatg agctgtatca gaaacacggg
    29161 cttggcgcgc gtgacgatgc gatgggaatg cagtatctga ttcctggctg gacgttcgat
    29221 aacaagcgcc cgtgcatggt gcgttaagac agaaaaccaa aaccggctct atctggagcc
    29281 ggcttgagac tgatgctaaa cgcaaaatag cctgttgtgc tatgtatcag gtctacatca
    29341 ttttttaatt cattgaattt aattggtttt gtaggccgga taaggcgtta acgccgcatc
    29401 cggcataaac acaggcactt tgtcaccaat ctgaaaccag cgtcataaaa agccgattgc
    29461 tactcgacaa gacgtccgtt acccgagaat tttgctggct tcgcgcgcta cattatccat
    29521 ctcgtccaac agttccagca gctccggctc caaatcttct tctttagtac cgctacgtag
    29581 ctgttgttcg atcagttggc agagattctt catacgcggc acaccgctat agccgcaact
    29641 gccatgcagt ttatgaatca aatcaaccag gccttccggg ttttctccaa ccagctgttc
    29701 ctcaactttg ttgcgaactt caggcaggaa atcgagtaac atttgcagca tatcgcgcgc
    29761 taaatcggtt tttcctgctg cctggcgtag tgccagttgc cagtcgaggg tcgcattcgg
    29821 gttcaccaca atttcgttga cttcgggcgt cacgacgcga gaggaaatac cgctgccagg
    29881 cttgtagcgc aacaacaaat tatgcaatcg ctcttcttca atcggtttcg ccagataatc
    29941 gctcatccct gcgccaagca gcttctcttt ttgcccggcc attgcatgcg ccgttaccgc
    30001 gataaccggc gtttgttgct gatgggggag ctggtggatg agctcgcagg cccgaatgcc
    30061 atccatgtca ggcatttgaa tatccattaa gatcaaatcg aacggcatct gtttcgcccg
    30121 ttcaaccgcc tgatgcccgc tatcgcaaag ttccacatgt tgcaccatat cttccagcaa
    30181 tgcgccgata agtttcaggt tagcggggtt gtcatcaact gccatgactg tcattgccag
    30241 cttgctttca tcggttacag gcaaaagcgt gttttgtttg tgatgacaaa attccgtcag
    30301 ggcaggcaac aggcgcgtag gtgttaatgg tttcagcaga cacgcgccga taccatcttg
    30361 cttgagtttt tctgcattga cttgtgcatg gcaaggaagt gccagcatca ggaaatcggt
    30421 catcgatacc gctttcgcta atcgctcatg ttgcattgtt agcggctcgc ggaaggtcac
    30481 cgcgatgcct aacaacatca tgtcgtaatg cgcgggaggc agcgcggaga acgttgggct
    30541 ataaaccact tccagcggcg tttcacttaa aatatccagc gtgcattgcg ctgctgcgga
    30601 gtttggttcg acataggcca ggcgcttacc ggcgaggcac tgggtggatg gcccttcgat
    30661 aataatgttc gggttcagat cgagattaat gtggaaccag aaagttgaac cgcgattcgg
    30721 ctggctatgg aacgaaatat cgccgcccat ttcattaacc agtttttgtg taatcaccag
    30781 ccccagaccg gtgccaccat gacggcggga aatactggca tcagcctgtc ggaaggcctg
    30841 gaataagcgc gattgatcgc gttcaggaat accaatgccg gtatcccgaa tctgcacttc
    30901 aatctgcact ttggtattac tcagcgcacg tttttctacc agaatatcaa tgttgccatt
    30961 ctcggtgaat ttaattgcat tccccaccag gttagtgatg atttgctgta atcgcagtgg
    31021 gtcgccgatc acgttatcag gcacgtcgct tttaatattg agcgtcagtt ccaacccttt
    31081 atcgtgagaa gaatgtgcca gcagagtaac gacttcatcc agcgtgctgc gtagtgggaa
    31141 tggaatactt tccagaatca gcttacctgc ttccagtttc gagaagtcga gaacatcatt
    31201 aataattgcc agcaaattat ttgccgaacg ttcaatcgta ttcaggtgat cgcgctgcgt
    31261 tggtgttaat tctgttttca gcgtcaggcg ggtaaagcca ataacaccat tcagtggtgt
    31321 acgcagctcg tgtgacatat ttgccagaaa ctcggattta atacgcgccg cttcctgggc
    31381 gcgctttttc gccagatcta actcaacgtt ctgaatttcc atctgctcca gcgtttcacg
    31441 cagatcggac gtcgcctggt cgatattgtg ctgcatctct tcgtgataag cagccagcga
    31501 cattgccatc gagttgatac cgtttttcag catatccagc tcgccgagca taaatccttc
    31561 cacccggctg tcgagttgcc cgcgacggat gcggtcgacg gtattcacca tgttgcgaat
    31621 cggaccggtt acatcgcgca ttaagcgcca gccaaaaata agcgcaatac cgatacaaaa
    31681 cagcatcatc acgctggaaa taaagatctc tttatattgc tgcaagcgaa ccgatttaag
    31741 atccagctcc agcgcaatat atcccaacat attttgacta tttttggcat cgctactggg
    31801 cgattcgtcg ggggagtaac tctcagaaat aatcggcgtg cggaggatca taatatcgcc
    31861 gtcacgagtg acagtgagct ggcgaggaaa cggcacgttg ctgccgagct gcattgatga
    31921 gggatcaaga tgaaaattgg aggtgacaaa gagtcggtta ttttcatcat aaaccgaaat
    31981 cgcgcgaaca atatcggaat ggcgacgatg cagtacgctt attaactgac cgatagattc
    32041 gcgattttgc aggctcatgc catattcagt agaaactgca agcggctcaa taatgctggc
    32101 accggcatct tccagttgac gctgcaagtc gttatagcga tgcacgacga aaaagatact
    32161 cagcaataaa ccaataagga cggtcggtgc caggatcaga atcatcatgc gtgcgcgcag
    32221 gctgtagttg gtcatggagt tccgttatgg gacaattaag gtcacactgt taaattgaga
    32281 aagtctcgcc aatggcgcaa ttctactctg caaaacgacg cacgacgacg cgtcagatca
    32341 taaccgtttc agtcaacgac ctcgactctt ttggtcaggg cgtggcgcga cataacggca
    32401 aaacgttatt tatccccgga ttattgccgc aggaaaacgc ggaagttgct gttactgaag
    32461 ataaaaaaca gtatgcccgc gctaaagtcg tacgccggtt aagcgatagc ccggaacgcg
    32521 aaacaccacg ctgtcctcat tttggcgtat gcggcggctg tcagcaacaa cacgccagcg
    32581 tggatttaca gcagcgaagc aaaagtgcgg cactcgcccg attaatgaaa cacgatgtct
    32641 ctgaagtgat cgccgatgtt ccctggggct atcgccgccg cgcgcgttta agtctgaact
    32701 acttaccgaa aacacagcaa cttcagatgg ggtttcgcaa agcgggctcc agtgacattg
    32761 tcgacgttaa acaatgcccc attttagtgc cccaacttga agcattgctg cccaaagtca
    32821 gggcatgcct gggcagctta caagttatgc gccatcttgg tcatgttgaa ctggtacagg
    32881 caaccagcgg cacgctgatg attttgcgcc ataccgcacc gctaagttcg gcagatcgcg
    32941 aaaaactgga acgcttttcg cattctgaag gcctggatct gtatctcgcc cccgatagtg
    33001 agatactcga aaccgtctct ggtgagatgc cctggtatga ctcaaacggg ttgcgcttaa
    33061 cttttagccc gcgcgatttt attcaggtca atgcgggtgt gaaccaaaaa atggtagcgc
    33121 gtgcgttgga atggctggat gtgcaacctg aagatcgcgt actggatctg ttctgcggta
    33181 tgggcaactt tacactgcca ttggcgaaac aagcagccag tgtggtcggt gtagaaggtg
    33241 ttccggcgct ggtggaaaaa ggccagcaga atgcgcgtct taatggttta cagaatgtga
    33301 cgttttatca cgaaaatctt gaagaagatg tcacaaagca gccgtgggcg aaaaacggct
    33361 tcgataaagt gttgctggac ccggcgcgag caggtgccgc aggtgttatg cagcaaatta
    33421 taaaactgga acctattcgt atagtttatg tatcctgtaa ccctgcaacg ctggctcggg
    33481 atagcgaagc gttattaaaa gcaggatata ccattgcgcg actggcgatg ctggatatgt
    33541 tcccacacac gggacatctg gaatcgatgg tacttttctc gcgcgttaaa tagttacgat
    33601 ttgccgattt cggcaggtct ggtccctaaa ggagaggaca atggttgcgg taagaagtgc
    33661 acatatcaat aaggctggtg aatttgatcc ggaaaaatgg atcgcaagtc tgggtattac
    33721 cagccagaag tcgtgtgagt gcttagccga aacctgggcg tattgtctgc aacagacgca
    33781 ggggcatccg gatgccagtc tgttattgtg gcgtggtgtt gagatggtgg agatcctctc
    33841 gacattaagt atggacattg acacgctgcg ggcggcgctg ctgttccctc tggctgatgc
    33901 caacgtagtc agcgaagatg tgctgcgtga gagcgtcggt aagtcggtcg ttaaccttat
    33961 tcacggcgtg cgtgatatgg cggcgatccg ccagctgaaa gcgacgcaca ctgattctgt
    34021 ttcctccgaa caggtcgata acgttcgccg gatgttattg gcgatggtcg atgattttcg
    34081 ctgcgtggtc atcaaactgg cggagcgtat tgctcatctg cgcgaagtaa aagatgcgcc
    34141 ggaagatgaa cgcgtactgg cggcaaaaga gtgcaccaat atctacgcgc cgttggcgaa
    34201 ccgtcttggg attgggcaac tgaaatggga gctggaagat tactgcttcc gttatctcca
    34261 cccgaccgaa tacaaacgca tcgcaaaact gctgcatgaa cgccgtctcg accgcgaaca
    34321 ctatatcgaa gagtttgtcg gccatctgcg cgctgagatg aaagctgaag gcgttaaagc
    34381 tgaagtgtat ggtcgtccga aacacatcta cagtatctgg cgcaaaatgc agaaaaagaa
    34441 cctcgccttc gatgagctgt ttgatgtgcg tgcggtacgt attgtcgccg agcgtttaca
    34501 ggattgttat gccgcactgg ggatagtgca cactcactat cgccacctgc cggatgagtt
    34561 tgacgattat gtcgctaacc cgaaaccaaa cggttatcag tctattcata ccgtggttct
    34621 ggggccaggt ggcaaaaccg ttgagatcca aatccgcacc aaacagatgc atgaagatgc
    34681 agagttgggt gttgctgcgc actggaaata taaagagggc gcggcagctg gcggcgcacg
    34741 ttcgggacat gaagaccgga ttgcctggct gcgtaaactg attgcgtggc aggaagagat
    34801 ggctgattcc ggcgaaatgc tcgacgaagt acgcagccag gtctttgacg accgggtgta
    34861 tgtctttacg ccgaaaggtg atgtcgttga tttgcctgcg ggatcaacgc cgctggactt
    34921 cgcttaccac atccacagtg atgtcggaca ccgctgcatc ggggcaaaaa ttggcgggcg
    34981 cattgtgccg ttcacctacc agctgcagat gggcgaccag attgaaatta tcacccagaa
    35041 acagccgaac cccagccgtg actggttaaa cccaaacctc ggttacgtca caaccagccg
    35101 tgggcgttcg aaaattcacg cctggttccg taaacaggac cgtgacaaaa acattctggc
    35161 tgggcggcaa atccttgacg acgagctgga acatctgggg atcagcctga aagaagcaga
    35221 aaaacatctg ctgccgcgtt acaactttaa tgatgtcgac gagttgctgg cggcgattgg
    35281 tggcggggat atccgtctca atcagatggt gaacttcctg caatcgcaat ttaataagcc
    35341 gagtgccgaa gagcaggacg ccgccgcgct gaagcaactt cagcaaaaaa gctacacgcc
    35401 gcaaaaccgc agtaaagata acggtcgcgt ggtggtcgaa ggtgttggca acctgatgca
    35461 ccacatcgcg cgctgctgcc agccgattcc tggagatgag attgtcggct tcattaccca
    35521 ggggcgcggt atttcagtac accgcgccga ttgcgaacaa ctggcggaac tgcgctccca
    35581 tgcgccagaa cgcattgttg acgcggtatg gggtgagagc tactccgccg gatattcgct
    35641 ggtggtccgt gtggtggcta atgatcgtag tgggttgtta cgtgatatca cgaccattct
    35701 cgccaacgag aaggtgaacg tgcttggcgt tgccagccgt agcgacacca aacagcaact
    35761 ggcgaccatc gacatgacca ttgagattta caacctgcaa gtgctggggc gcgtgctggg
    35821 taaactcaac caggtgccgg atgttatcga cgcgcgtcgg ttgcacggga gttaggccga
    35881 aatttgctcg tatctacaat gtagattgat atatactgta tctacatatg atagcggttt
    35941 gaggaaaggg ttatgatcca cagtagcgta aagcgttggg gaaattcacc ggcggtgcgg
    36001 atcccggcta cgttaatgca ggcgctcaat ctgaatattg atgatgaagt gaagattgac
    36061 ctggtggatg gcaaattaat tattgagcca gtgcgtaaag agcccgtatt tacgcttgct
    36121 gaactggtca acgacatcac gccggaaaac ctccacgaga atatcgactg gggagagccg
    36181 aaagataagg aagtctggta atggtaagcc gatacgtacc cgatatgggc gatctgattt
    36241 gggttgattt tgacccgaca aaaggtagcg agcaagctgg acatcgtcca gctgttgtcc
    36301 tgagtccttt catgtacaac aacaaaacag gtatgtgtct gtgtgttcct tatacaacgc
    36361 aatcaaaagg atatccgttc gaagttgttt tatccggtca ggaacgtgat ggcgtagcgt
    36421 tagctgatca ggtaaaaagt atcgcctggc gggcaagagg agcaacgaag aaaggaacag
    36481 ttgccccaga ggaattacaa ctcattaaag ccaaaattaa cgtactgatt gggtagtgtt
    36541 actaactcac ttctattctg gtcacaggtt tccacctgac agacccgcaa ttttcaggac
    36601 aattcaatga atcaaatcga ccgtttgctc actattatgc agcgcctgcg cgatccggaa
    36661 aacggctgcc cgtgggataa agagcagaca tttgccacca ttgcgcctta cacccttgaa
    36721 gaaacctacg aagtgctgga cgccatcgcc cgtgaagatt ttgacgatct tcgcggtgaa
    36781 ctgggcgatc tgctattcca ggtggtgttt tacgcgcaaa tggctcagga agaagggcgc
    36841 tttgacttta atgatatttg cgctgctatt agcgataaat tagagcgtcg ccatccgcat
    36901 gtttttgctg atagttctgc cgaaaacagt agtgaagtgc ttgcccgttg ggagcaaatc
    36961 aaaaccgaag agcgcgcgca gaaagcgcag cattcggcgc tggacgatat tcctcgtagt
    37021 ttaccggctt taatgcgtgc gcaaaaaatc cagaaacgtt gcgccaacgt tggcttcgat
    37081 tggacgacgc ttggtccggt agtcgataaa gtctacgaag agatcgacga ggtgatgtac
    37141 gaagcgcggc aggctgttgt cgaccaggct aaactggagg aggaaatggg ggacctgctg
    37201 tttgccacgg tcaatctggc tcgtcattta gggacaaaag cggaaatcgc attgcaaaaa
    37261 gcgaacgaaa aatttgagcg tcgttttcgc gaagtggagc gtattgttgc cgcgcgtgga
    37321 ctggaaatga caggtgttga cctcgaaaca atggaagaag tctggcaaca ggtaaaacgg
    37381 caggaaattg atctctaagg gaattacgcg gtcaagcgcc atttgtgtca ttttttaaat
    37441 gacaagcgct tgatttgcgt caaaaacatt taccccaaag gggctatttt ctcactcctg
    37501 atttcaatag tgcgctggcg aagaggaggg ataatgaaag tttgtggcac aggtcatgtt
    37561 cgggtatact gctttcccgt cctggttatt ccatcgtctt ttcaacctaa cttctcaggt
    37621 tcagcatgac aacgaactat atttttgtga ccggcggggt cgtatcctct ctgggtaaag
    37681 gcattgccgc agcctccctc gcagccattc ttgaagcccg tggcctcaat gtgaccatca
    37741 tgaaactgga tccgtacatc aacgtcgatc caggtactat gagcccaatc caacacgggg
    37801 aagtgttcgt tactgaagac ggcgctgaaa ccgacctgga cctggggcac tacgagcgtt
    37861 tcattcgtac caaaatgagc cgccgcaaca acttcaccac gggtcgtatc tactctgacg
    37921 ttctgcgtaa agaacgccgc ggtgactacc tcggcgcaac cgtgcaggtt attccgcaca
    37981 tcactaacgc aatcaaagag cgcgtgctgg aaggtggcga aggtcatgac gtagtactgg
    38041 tagaaatcgg cggtacagta ggtgatatcg aatccttgcc gttcctcgaa gcgattcgcc
    38101 agatggctgt tgaaattggc cgtgagcaca ctctgtttat gcacctgacg ctggtgccgt
    38161 acatggcagc gtctggtgaa gtcaaaacca aaccgactca gcactctgta aaagagctgc
    38221 tctccatcgg tatccagcct gacatcctga tttgtcgttc agatcgcgct gttccggcga
    38281 acgaacgtgc gaagattgca ttgttctgta atgttccgga aaaagcggtt atttctctga
    38341 aagacgtcga ttccatctat aaaattccgg gcctgttgaa atctcagggg ctggacgatt
    38401 atatttgtaa acgattcagc ttaaactgcc cggaagcgaa tctgtccgaa tgggaacagg
    38461 ttatcttcga agaagcgaat ccggtaagtg aagtcaccat cggtatggtc ggcaagtaca
    38521 ttgaactgcc ggatgcttat aaatcagtga tcgaagcact gaaacacggt gggctgaaga
    38581 atcgtgtcag cgtcaacatc aaactgatcg attcacaaga tgttgaaacg cgcggcgttg
    38641 aaatccttaa aggtctggac gcaatcctcg tacctggcgg ttttggctat cgtggcgtag
    38701 aaggcatgat tacgaccgcg cgttttgcgc gtgagaacaa tattccttat ctgggcattt
    38761 gcctgggtat gcaggtggcg ttaattgatt acgctcgcca tgttgccaac atggagaacg
    38821 ccaactctac ggaatttgtg ccagaatgta agtacccggt tgtggcgctg attaccgagt
    38881 ggcgcgatga aaacggcaac gttgaagttc gtagcgagaa gagcgatctc ggcggaacca
    38941 tgcgtctcgg cgcacagcag tgccagttgg ttgacgatag cctggttcgc cagctgtaca
    39001 atgcgccgac aattgttgag cgtcatcgtc accgttacga agtcaacaac atgctgttga
    39061 aacagattga agatgcaggt ctgcgcgttg cgggccgttc cggggatgat cagttggtcg
    39121 agatcatcga agttccgaat cacccgtggt tcgtggcttg ccagttccat ccggagttta
    39181 cttctactcc acgtgatggt cacccgctgt ttgcaggctt tgtgaaagcc gccagcgagt
    39241 tccagaaacg tcaggcgaag taagtaaaaa agttagaacg gcaacgcgta ccctgggtac
    39301 gcgttgtttg tctggagttt cagtttaact agtgacttga ggaaaaccta atgtccaaaa
    39361 tcgtaaaaat catcggtcgt gaaatcatcg actcccgtgg taacccgact gttgaagccg
    39421 aagtacatct ggagggtggt ttcgtcggta tggcagctgc tccgtcaggt gcttctactg
    39481 gttcccgtga agctctggaa ctgcgcgatg gcgacaaatc ccgtttcctg ggtaaaggcg
    39541 taaccaaagc tgttgctgcg gtaaacggcc cgatcgctca ggcgctgatt ggcaaagatg
    39601 ctaaagatca ggctggcatt gacaagatca tgatcgacct ggacggcacc gaaaacaaat
    39661 ccaaattcgg cgcgaacgca atcctggctg tatctctggc taacgccaaa gctgctgcag
    39721 ctgctaaagg gatgccgctg tacgagcaca tcgctgaact gaacggtact ccgggcaaat
    39781 actctatgcc ggttccgatg atgaacatca tcaacggtgg tgagcacgct gacaacaacg
    39841 ttgatatcca ggaattcatg attcagccgg ttggcgcgaa aactgtgaaa gaagccatcc
    39901 gcatgggttc tgaagttttc catcacctgg caaaagttct gaaagcgaaa ggcatgaaca
    39961 ctgctgttgg tgacgaaggt ggctatgcgc cgaacctggg ttccaacgct gaagctctgg
    40021 ctgttatcgc tgaagctgtt aaagctgctg gttatgaact gggcaaagac atcactctgg
    40081 cgatggactg cgcagcttct gaattctaca aagacggtaa atacgttctg gctggcgaag
    40141 gcaacaaagc gttcacctct gaagaattca ctcacttcct ggaagaactg accaaacagt
    40201 acccgatcgt ttctatcgaa gacggtctgg acgaatctga ctgggacggt ttcgcatacc
    40261 agaccaaagt tctgggcgac aaaatccagc tggttggtga cgacctgttc gtaaccaaca
    40321 ccaagatcct gaaagaaggt atcgaaaaag gtatcgctaa ctccatcctg atcaaattca
    40381 accagatcgg ttctctgacc gaaactctgg ctgcaatcaa gatggcgaaa gatgctggct
    40441 acactgcagt tatctctcac cgttctggcg aaactgaaga cgctaccatc gctgacctgg
    40501 ctgttggtac tgctgcaggc cagatcaaaa ctggttctat gagccgttct gaccgcgttg
    40561 ctaaatacaa ccagctgatt cgtatcgaag aagctctggg cgaaaaagca ccgtacaacg
    40621 gtcgtaaaga gatcaaaggc caggcataag actgacttta tctgatttaa aaatgccagc
    40681 ctccgggctg gcatttttta tggttgagtt accagcggcc ggatgcgccg ccacctccag
    40741 aagaaccgcc accgccgcta aatccgccgc cacttgagcc tccaccggaa ccgctgttgt
    40801 ctgaatgata acttttacct gatgctcttt tctgaagtac acataaacag gtaaagacca
    40861 taaatatggt gaaagtaaaa aagaaggaaa atacagcaat ggaaaaaatt ttatcggaga
    40921 acaggaataa atctaaaaga tagattgctg aaatatagac tccactttta catgcgcgac
    40981 aaaaattact gccttgatga aaaaaccaga aagggaagaa catcactgca aatacccaaa
    41041 aaatagcaaa gtaataatga tcactggaag acgctgattc actttctgaa ggattagtcg
    41101 gatattggtg ttgtgaagtg agttggttat tcaaagcgtt tatagctagc tctaatccct
    41161 gagcaaattt ttgttgttta aatgcgggta tcatgttgct acggatgata tctctagcca
    41221 gagcatcggt taccttttct tccagcccat aacctacctg gatgcggaca gtacgatctg
    41281 accaggcaac aatgatcagt atcccatcat tacgtttggc atctcctaaa cgccaattgt
    41341 caaaaactct tgtagcatat tgttcaattg tctcgtcttt ggttgtggga acaattaata
    41401 cagcaatttg cacttgggta ttattgaaaa tagattctaa cttgtgcgtc agttctttct
    41461 tttcttgtga gtttaagcta ctcgtaagat ccgtaacttg ttactgtaat tgtgggacaa
    41521 tagttggttg ggctgcaaca aaggaacata cgaaagtgaa cattagtatg aaatagcgca
    41581 ttataaattt ctcattaaat ctggataaat aaattgacgg cggcatcggt aattttagat
    41641 tcgttatcat taaaaaccaa tacagaaaaa ataaatataa ttttggtgaa tgttttgaca
    41701 tgattaaatt cattttcttt attttcttct gacataatac gttactccct ttaattatta
    41761 acgatatgtt tcgcattttt ctttaaatct gcctgtaaca gaatgttaat aagacatgat
    41821 tgttgcggca ggatctgcaa taattaacaa ttatccactt cacagagaat gctatgcagt
    41881 acccgattaa cgagatgttc cagaccctgc aaggtgaggg ttactttacc ggcgtccccg
    41941 ccatttttat tcgtttacag ggatgcccgg ttggctgtgc ctggtgcgac accaaacaca
    42001 cctgggaaaa gcttgaggat cgggaagtct cccttttcag cattctggcg aagaccaaag
    42061 agagtgataa gtggggggcg gcgagcagtg aagatttgct ggctgtcatt ggtcggcagg
    42121 gatataccgc gcggcatgtg gtgattacgg gtggtgagcc ttgcattcat gatttgctgc
    42181 cactgactga cctgctcgaa aagaacggtt ttagctgcca gatcgaaacc agtggaactc
    42241 atgaagtacg ctgcacaccg aatacctggg ttaccgtatc gccaaagctg aacatgcgcg
    42301 gcggctatga cgtgttgtca caggcactgg agcgagccaa cgaaatcaag catccagtgg
    42361 ggcgcgtacg tgatattgaa gcactggatg aactactggc aacgctgacc gatgataaac
    42421 cgcgagtcat tgcactgcag ccgattagcc aaaaggatga tgccacacgt ttgtgcattg
    42481 aaacctgcat tgcgcgtaat tggcgtttgt cgatgcaaac acataaatat ctaaatattg
    42541 cctgattaaa catttataag cgttataaat gggtggaacc tgtaaagact tctactcatt
    42601 tatattgttt gtcgcctctg aaaactcctc cattttaccc atccagggct aatcattagc
    42661 attctctaca aattctgtgg cattaatttt tcgctggagt gaaaattatt gcggtaaagt
    42721 ttggtagatt ttagtttgta tagagttatt ttaaatattt acctttttaa tcaatggatt
    42781 aagtgctctt taacataatg gatgtgttgt ttgtgtgata ctataaagtt ggtagattgt
    42841 gactggctta aaaaatcatt aattaataat aggttatgtt tacagagttc cccgcgccag
    42901 cggggataaa ccgccctcca gtactgtgat gcgatcttta agtccgagtt ccccgcgcca
    42961 gcggggataa accgactggt tctgtaaccc aaatttggga tcattcgagt tccccgcgcc
    43021 agcggggata aaccgacggg gacgaatcac agcagcgtta tcgtgatgag ttccccgcgc
    43081 cagcggggat aaaccgaaac cggacgaggg cgcgctaaaa cgtgattaga gttccccgcg
    43141 ccagcgggga taaaccgcct ctcaatttgt aacgtctgca atgtcaggcg agttccccgc
    43201 gccagcgggg ataaaccacc atataacccg ttatctcttt ctcaagtttt tatattagca
    43261 gtacttgtaa taagcaacat atccacgtaa cacctcatgt tcaaaatagt tctccatgcc
    43321 a
//
LOCUS       DAZFXT010000025        42985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.025, whole genome shotgun sequence.
ACCESSION   DAZFXT010000025 DAZFXT010000000
VERSION     DAZFXT010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.025"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..116
                     /locus_tag="ACHKW0_001494"
     CDS             <1..116
                     /locus_tag="ACHKW0_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO7474351.1"
                     /translation="SPTSTHNDIMGGGGMSYAYEYDVKYNICHLRKKSRMR"
     gene            complement(203..878)
                     /locus_tag="ACHKW0_001495"
                     /pseudo
     CDS             complement(203..878)
                     /locus_tag="ACHKW0_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1023..1631)
                     /gene="yiiX"
                     /locus_tag="ACHKW0_001496"
     CDS             complement(1023..1631)
                     /gene="yiiX"
                     /locus_tag="ACHKW0_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HFO7474352.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1815..2132)
                     /gene="metJ"
                     /locus_tag="ACHKW0_001497"
     CDS             complement(1815..2132)
                     /gene="metJ"
                     /locus_tag="ACHKW0_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HFO7474353.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2409..3569
                     /gene="metB"
                     /locus_tag="ACHKW0_001498"
     CDS             2409..3569
                     /gene="metB"
                     /locus_tag="ACHKW0_001498"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HFO7474354.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALADKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGYGAMLSFELDGDEQTLRRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3572..6004
                     /gene="metL"
                     /locus_tag="ACHKW0_001499"
     CDS             3572..6004
                     /gene="metL"
                     /locus_tag="ACHKW0_001499"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HFO7474355.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(6143..6277)
                     /locus_tag="ACHKW0_001500"
     CDS             complement(6143..6277)
                     /locus_tag="ACHKW0_001500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474356.1"
                     /translation="MKEKPKGVNRKMTNDGEEKSGKMKKLQDGKKKKIRYAKQTGLQI
                     "
     gene            6353..7243
                     /gene="metF"
                     /locus_tag="ACHKW0_001501"
     CDS             6353..7243
                     /gene="metF"
                     /locus_tag="ACHKW0_001501"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HFO7474357.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7572..9752
                     /gene="katG"
                     /locus_tag="ACHKW0_001502"
     CDS             7572..9752
                     /gene="katG"
                     /locus_tag="ACHKW0_001502"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HFO7474358.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9846..10751
                     /gene="yijE"
                     /locus_tag="ACHKW0_001503"
     CDS             9846..10751
                     /gene="yijE"
                     /locus_tag="ACHKW0_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HFO7474359.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(10778..11395)
                     /gene="yijF"
                     /locus_tag="ACHKW0_001504"
     CDS             complement(10778..11395)
                     /gene="yijF"
                     /locus_tag="ACHKW0_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HFO7474360.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11670..12773)
                     /gene="gldA"
                     /locus_tag="ACHKW0_001505"
     CDS             complement(11670..12773)
                     /gene="gldA"
                     /locus_tag="ACHKW0_001505"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HFO7474361.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12784..13446)
                     /gene="fsa"
                     /locus_tag="ACHKW0_001506"
     CDS             complement(12784..13446)
                     /gene="fsa"
                     /locus_tag="ACHKW0_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7474362.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGIVEEAKRLRDAIPGIVVKIPVTSEGLAAIKMLKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13458..15959)
                     /gene="ptsP"
                     /locus_tag="ACHKW0_001507"
     CDS             complement(13458..15959)
                     /gene="ptsP"
                     /locus_tag="ACHKW0_001507"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7474363.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16268..17347
                     /gene="frwC"
                     /locus_tag="ACHKW0_001508"
     CDS             16268..17347
                     /gene="frwC"
                     /locus_tag="ACHKW0_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HFO7474364.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17362..17682
                     /gene="frwB"
                     /locus_tag="ACHKW0_001509"
     CDS             17362..17682
                     /gene="frwB"
                     /locus_tag="ACHKW0_001509"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7474365.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            17733..20030
                     /gene="pflD"
                     /locus_tag="ACHKW0_001510"
     CDS             17733..20030
                     /gene="pflD"
                     /locus_tag="ACHKW0_001510"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7474366.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAEHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            19996..20874
                     /gene="pflC"
                     /locus_tag="ACHKW0_001511"
     CDS             19996..20874
                     /gene="pflC"
                     /locus_tag="ACHKW0_001511"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418387.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HFO7474367.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLNIRQIHLLPFHQYGEPKYRLLGKTWSMKEVSAPSSADVAT
                     MREMAERAGFQVTVGG"
     gene            20876..21217
                     /gene="frwD"
                     /locus_tag="ACHKW0_001512"
     CDS             20876..21217
                     /gene="frwD"
                     /locus_tag="ACHKW0_001512"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HFO7474368.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21204..22055)
                     /gene="yijO"
                     /locus_tag="ACHKW0_001513"
     CDS             complement(21204..22055)
                     /gene="yijO"
                     /locus_tag="ACHKW0_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7474369.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22204..23937)
                     /gene="eptC"
                     /locus_tag="ACHKW0_001514"
     CDS             complement(22204..23937)
                     /gene="eptC"
                     /locus_tag="ACHKW0_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO7474370.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSKQTDKQFYMNQQRTQSAREYDTNVLKPFQEVLKDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLNAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     NPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24120..26771)
                     /gene="ppc"
                     /locus_tag="ACHKW0_001515"
     CDS             complement(24120..26771)
                     /gene="ppc"
                     /locus_tag="ACHKW0_001515"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HFO7474371.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27123..28274)
                     /gene="argE"
                     /locus_tag="ACHKW0_001516"
     CDS             complement(27123..28274)
                     /gene="argE"
                     /locus_tag="ACHKW0_001516"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HFO7474372.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            28428..29432
                     /gene="argC"
                     /locus_tag="ACHKW0_001517"
     CDS             28428..29432
                     /gene="argC"
                     /locus_tag="ACHKW0_001517"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HFO7474373.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29440..30216
                     /gene="argB"
                     /locus_tag="ACHKW0_001518"
     CDS             29440..30216
                     /gene="argB"
                     /locus_tag="ACHKW0_001518"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HFO7474374.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30277..31650
                     /gene="argH"
                     /locus_tag="ACHKW0_001519"
     CDS             30277..31650
                     /gene="argH"
                     /locus_tag="ACHKW0_001519"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HFO7474375.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            32149..33363
                     /gene="manD"
                     /locus_tag="ACHKW0_001520"
     CDS             32149..33363
                     /gene="manD"
                     /locus_tag="ACHKW0_001520"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-mannonate dehydratase ManD"
                     /protein_id="HFO7474376.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            33430..34713
                     /locus_tag="ACHKW0_001521"
     CDS             33430..34713
                     /locus_tag="ACHKW0_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474377.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAASPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            34965..35882
                     /gene="oxyR"
                     /locus_tag="ACHKW0_001522"
     CDS             34965..35882
                     /gene="oxyR"
                     /locus_tag="ACHKW0_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HFO7474378.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(35865..37265)
                     /gene="sthA"
                     /locus_tag="ACHKW0_001523"
     CDS             complement(35865..37265)
                     /gene="sthA"
                     /locus_tag="ACHKW0_001523"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003957 - NAD(P)+ transhydrogenase
                     (Si-specific) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HFO7474379.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            37596..38246
                     /gene="fabR"
                     /locus_tag="ACHKW0_001524"
     CDS             37596..38246
                     /gene="fabR"
                     /locus_tag="ACHKW0_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HFO7474380.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            38246..38605
                     /gene="yijD"
                     /locus_tag="ACHKW0_001525"
     CDS             38246..38605
                     /gene="yijD"
                     /locus_tag="ACHKW0_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HFO7474381.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(38645..39745)
                     /gene="trmA"
                     /locus_tag="ACHKW0_001526"
     CDS             complement(38645..39745)
                     /gene="trmA"
                     /locus_tag="ACHKW0_001526"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030697 - tRNA
                     (uracil(54)-C5)-methyltransferase activity, S-adenosyl
                     methionine-dependent [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HFO7474382.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      39859..40049
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            40114..41958
                     /gene="btuB"
                     /locus_tag="ACHKW0_001527"
     CDS             40114..41958
                     /gene="btuB"
                     /locus_tag="ACHKW0_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015420 - ABC-type vitamin B12 transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HFO7474383.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            41903..42760
                     /gene="murI"
                     /locus_tag="ACHKW0_001528"
     CDS             41903..42760
                     /gene="murI"
                     /locus_tag="ACHKW0_001528"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HFO7474384.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9805 a        10707 c        11823 g        10650 t
ORIGIN      
        1 aaagcccaac atcaacccac aatgacataa tggggggcgg gggaatgtca tatgcctatg
       61 aatatgatgt aaaatataat atatgtcatt tgagaaaaaa gagcagaatg aggtaaaaat
      121 ctttggctgt gtattcagtc tggtaatcat ccaaggaaag cttatcagaa gggtgatgac
      181 tccaattagt agaacatata ttttcgataa aagcaccgat gactttttca tgaagttcta
      241 cagaacgcga gaaacagatg gttctgcggg ccagacgttt gattcgggtt ctcagggtca
      301 gattgttacg ctcaatgtgc tgagtaaaaa tcttaccagt cagatgcttc tccttcggta
      361 actctctagc gtaactaccc cattcgtcag tggtcaacat gccgatggta aaagggtaag
      421 cagcgcccgc aattcacgac aggtttcgtc agtccggggg ccaaaagtgt aggccagaac
      481 accaccagtt ttcgtgttgt acacgtacca gagccagtgc tgtcgcgctt tactgccgac
      541 aaagctccat tgctgactaa gtttgcagat gagtgccaca tcagcatgag cgaccgggga
      601 tgacgttatt cttcgtggcg agagtttttt aaagttcgga tgactgtatt aatgccgatt
      661 ttcagcgtac gggcagtatc gcgaaccccg gcaccgttga aagtcatttc agcgatttgg
      721 tctttaactc ccggtttacg gggctcgtaa cagtaactga gctgaaatac ccggtgacag
      781 tcacggccgc ggaatcgatc atgtcttttt gggttctgcc cgtgacggta aacctgaacg
      841 gactggcaac gggggcaatg aaggttaact gtgaccatga aacaacctta aaagcgagta
      901 ttatatcctt aattcaacta attggaggca tcaccgttta ccggagcgac agcttgcgga
      961 aaggtcgtgc aactacaaag caacaaacag actacggcgg gaattatttt tttcatcatc
     1021 cattaccacg aaaacagcgg ccactctttg gcaaccgtgg tgagttgtgg cgcgtcgaaa
     1081 acggcttgtg gtgaaactac cgtttcctca agcggaatat ttttaccgta gcgttctttg
     1141 agcttcgcct ggaccagcgg gttgctgaga tcaaactctt tcaacttctg ctgttcgccc
     1201 acgcgcattc ccagcgcgtt ctggtaaacc ttccacacca cttccgaaca gtactggcga
     1261 tcgtcgctcc atgagaagct gaaatcatac ggtttaccga gataacgttt tgccgtttgc
     1321 gccagttttt gctgttgttc aacactcagt ccgccttcaa cgcggcgaac gacgtatttg
     1381 cccttttcac catgggcgat ccactgcttg agcggggtgt atttcaccgg gccgaccgct
     1441 tcaaaaacgt agggcttttt gttgcgtatc accagcatac cggtgtggct gtaatcggaa
     1501 tgggttgcca gttggatcgc tttactttgc gatgagcgag agatttgaaa gatgatgtcg
     1561 ccagtttgcg gctgccaggc aaaggcaggt acagaaacca gcaggctgag gatcagcagc
     1621 ctgtttttca tagttaaacg tccatgtata aaaagcggtg ggtcgcagac aacgtgctcg
     1681 ttgtttatgc cggatgcggc gtgaacgcct tatccggcct acaagttcgt gcaaattcaa
     1741 taaattgcaa tatgacgtag gcctgataag cgtagcgcat caggcggttc cactccgcgc
     1801 cgctcttttc cgctttagta ttcccacgtc tccgggttaa tccccatctc acgcatgatc
     1861 tcttttgccg cttccgggat ttcgtcgctg cgctctttac gcagatcggc atcatccggc
     1921 aaaggttgcc cggtaaaggc atgcagaaac gcttcgcaca gcagctcgct gttggtagcg
     1981 tgacgcaggt tgttcacctg acgacgcgtg cgttcatcgg tgaggatttt taacacctta
     2041 agaggaatgg aaaccgtaat ctttttgact tgttcactct tcttgccgtg ctcagcgtat
     2101 gggctgatat attcgccgct ccattcagcc atgagatact taatcctctt cgtcaataaa
     2161 ttgagaccag accacacagt taatgtgggc actggccgta aacccgcata gtttaccgta
     2221 caggcgctac cgtgacatcg ggtaatccac ctgctggcgt gataatgcat ataattttaa
     2281 cggctatttg ggatttgctc aatctatacg caaagaagtt tagatgtcca gatgtattga
     2341 cgtccattac cacaatgttt actctggtgc ctgacatttc accaataaag cccagggaac
     2401 atcatcacat gacgcgtaaa caggccacca tcgcagtgcg tagcgggtta aatgacgacg
     2461 aacagtatgg ttgcgttgtc ccacccatcc atctttccag tacctataac tttaccggat
     2521 ttaatgaacc gcgcgcgcat gattactcgc gtcgtggcaa cccaacgcgc gatgtggttc
     2581 agcgagcact ggcagaactg gaaggcggtg ctggtgcagt acttactaat accggcatgt
     2641 ccgcgattca cctggtaacg accgtctttt tgaaacctgg cgatctgctg gtagcgccgc
     2701 acgactgcta cggcggtagc tatcgcctgt tcgacagcct ggcgaaacgc ggttgctatc
     2761 gcgtgttgtt tgttgatcaa ggcgatgaac aggcattacg ggcagcgctg gcagataaac
     2821 ccaaactggt actggtagaa agcccaagta atccattgtt acgcgtcgtg gatattgcga
     2881 aaatctgcca tctggcaagg gaagtcgggg cggtgagcgt ggtggataac accttcttaa
     2941 gcccggcatt acaaaatccg ctggcattag gtgccgatct ggtgttgcat tcatgcacga
     3001 aatatctgaa cggtcactca gacgtggtgg ccggcgtggt gattgctaaa gacccggacg
     3061 ttgtcactga acttgcctgg tgggcaaaca atattggcgt gacgggcggc gcgtttgaca
     3121 gctatctgct gctacgcggg ttgcgaacgc tggtgccgcg catggagttg gcccagcgta
     3181 acgcgcaggc gattgtgaaa tatctgcaaa cccagccgtt ggtgaaaaaa ctgtatcacc
     3241 cgtcgctgcc ggaaaatcag gggcatgaaa ttgccgcgcg ccagcaaaaa ggctatggcg
     3301 caatgttgag ttttgaactg gatggcgatg agcagacgct gcgtcgtttc ctgggtggac
     3361 tgtcgttgtt tacgctggcg gaatcattag ggggagtgga aagtttaatc tctcacgccg
     3421 caaccatgac acatgcaggc atggcaccag aagcgcgtgc tgccgccggg atctccgaga
     3481 cgctgctgcg tatctccacc ggtattgaag atggcgaaga tttaattgcc gacctggaaa
     3541 atggcttccg ggctgcaaac aaggggtaaa aatgagtgtg attgcgcagg caggggcgaa
     3601 aggtcgtcag ctgcataaat ttggtggcag tagtctggct gatgtgaagt gttatttgcg
     3661 tgtcgcgggc attatggcgg agtactctca gcctgacgat atgatggtgg tttccgccgc
     3721 cggtagcacc actaaccagt tgattaactg gttgaaacta agccagaccg atcgtctctc
     3781 tgcgcatcag gttcaacaaa cgctgcgtcg ctatcagtgc gatctgatta gcggtctgct
     3841 acccgctgaa gaagccgata gcctcattag cgcttttgtc agcgaccttg agcgcctggc
     3901 ggcgctgctc gacagcggta ttaacgacgc agtgtatgcg gaagtggtgg ggcacggtga
     3961 agtgtggtcg gcacgcctga tgtctgcggt acttaatcaa caagggctgc cagcggcctg
     4021 gcttgatgcc cgcgagtttt tacgcgctga acgcgccgca caaccgcagg ttgatgaagg
     4081 gctttcttac ccgttgctgc aacagctgct ggtgcaacat ccgggcaaac gtctggtggt
     4141 gaccggattt atcagccgca acaacgccgg tgaaacggtg ctgctggggc gtaacggttc
     4201 cgactattcc gcgacacaaa tcggtgcgct ggcgggtgtt tctcgcgtaa ccatctggag
     4261 cgacgtcgcc ggggtataca gtgccgaccc gcgtaaagtg aaagatgcct gcctgctgcc
     4321 gttgctgcgt ctggatgagg ccagcgaact ggcgcgcctg gcggctcccg ttcttcacgc
     4381 ccgtacttta cagccggttt ctggcagcga aatcgacctg caactgcgct gtagctacac
     4441 gccggatcaa ggttccacgc gcattgaacg cgtgctggcc tccggtactg gtgcgcgtat
     4501 tgtcaccagc cacgatgatg tctgtttgat tgagtttcag gtgcccgcca gtcaggattt
     4561 caaactggcg cataaagaga tcgaccaaat cctgaaacgc gcgcaggtac gcccgctggc
     4621 ggttggcgta cataacgatc gccagttgct gcaattttgc tacacctcag aagtggccga
     4681 cagtgcgctg aaaatcctcg acgaagcggg attacctggc gaactgcgcc tgcgtcaggg
     4741 gctggcgctg gtggcgatgg tcggtgcagg cgtcacccgt aacccgctgc attgccaccg
     4801 cttctggcag caactgaaag gccagccggt cgaatttacc tggcagtccg atgacggcat
     4861 cagcctggtg gcagtactgc gcaccggccc gaccgaaagc ctgattcagg ggctgcatca
     4921 gtccgtcttc cgcgcagaaa aacgcatcgg cctggtattg ttcggtaagg gcaatatcgg
     4981 ttcccgttgg ctggaactgt tcgcccgtga gcagagcacg ctttcggcac gtaccggctt
     5041 tgagtttgtg ctggcaggtg tggtggacag ccgccgcagc ctgttgagct atgacgggct
     5101 ggacgccagc cgcgcgttag ccttcttcaa cgatgaagcg gttgagcagg atgaagagtc
     5161 gttgttcctg tggatgcgcg cccatccgta tgatgattta gtggtgctgg acgttaccgc
     5221 cagccagcag cttgctgatc agtatcttga tttcgccagc cacggtttcc acgtcatcag
     5281 cgccaacaaa ctggcgggag ccagcgacag caataaatat cgccagatcc acgacgcctt
     5341 cgaaaaaacc gggcgtcact ggctgtacaa tgctaccgtc ggagcgggct tgccgatcaa
     5401 ccataccgtg cgcgatctga tcgacagcgg cgatactatt ttgtcgatca gtgggatctt
     5461 ctccggcacg ctctcctggc tgttcctgca attcgacggt agcgtgccgt ttaccgagct
     5521 ggtggatcag gcgtggcagc aagggttaac cgagcctgac ccgcgtgatg acctttccgg
     5581 caaagacgta atgcgcaagc tggtgattct ggcgcgtgaa gcaggttaca acatcgaacc
     5641 ggatcaggta cgcgtggagt cgctggtgcc tgcccattgc gaaggcggca gcatcgacca
     5701 tttctttgaa aatggcgatg aacttaacga gcagatggtg caacggctgg aagcggcccg
     5761 cgaaatgggg ctggtgctgc gctacgtggc gcgtttcgat gccaacggca aagcgcgtgt
     5821 gggcgtggag gcggtgcgtg aagaccatcc gctggcatca ctgctgccgt gcgataacgt
     5881 ctttgccatc gaaagccgct ggtatcgcga caacccgctg gtgatccgtg ggcctggcgc
     5941 ggggcgcgac gtcaccgccg gggcgattca gtcggatatc aaccggctgg cacagttgtt
     6001 gtaatttcag aaatttaata atgcccggta ctcatgtttt cgggtttatg gtttctaatg
     6061 aaatatattg aattatcata ggattaggcc ggattaagcg tttacgacga atccggcaag
     6121 aagcaataag tacatggtta gtttatattt gcagtccggt ttgctttgca taccggattt
     6181 tctttttctt accatcctga agttttttca tcttccctga tttttcctca ccatcattgg
     6241 tcatttttcg gttgacgccc ttcggctttt ccttcatctt tacatctgga cgtctaaacg
     6301 gatagatgtg cacaacacaa catataacta caagcgattg atgaggtaag gtatgagctt
     6361 ttttcacgcc agccagcggg atgccctgaa tcagagcctg gcagaagtcc aggggcagat
     6421 taacgtttcg ttcgagtttt tcccgccgcg taccagtgaa atggagcaga ccctgtggaa
     6481 ctccatcgat cgccttagca gcctgaaacc gaagtttgta tcggtgacct atggcgcgaa
     6541 ctccggcgag cgcgaccgta cgcacagcat tattaaaggc attaaagatc gcactggtct
     6601 ggaagcggca ccgcatctta cttgcattga tgcgacgccc gacgagctgc gcaccattgc
     6661 acgcgactac tggaataacg gtattcgtca tatcgtggcg ctgcgtggcg atctgccgcc
     6721 gggaagtggt aagccggaaa tgtatgcttc tgacctggtg acgctgttaa aagaagtggc
     6781 agatttcgat atctccgtgg cggcgtatcc ggaggttcac ccggaagcaa aaagcgctca
     6841 ggcggatttg ctcaacctga aacgcaaagt cgatgccgga gccaaccgcg cgattactca
     6901 gttcttcttt gatgtcgaaa gctacctgcg ttttcgtgac cgctgcgtat cggcgggtat
     6961 tgatgtggaa attattccgg gaattttgcc ggtctcgaac tttaaacaag cgaagaaatt
     7021 tgccgatatg accaacgtgc gtattccggc gtggatggca caaatgtttg acgggctgga
     7081 cgacgatgcc gaaacccgca aactggtggg agcgaatatt gccatggata tggtgaagat
     7141 tttaagccgt gaaggagtga aagatttcca cttctatacg cttaaccgcg ctgaaatgag
     7201 ttacgcgatt tgccatacgc tgggtgttcg acctggttta taaatagtgt ggcttttgtg
     7261 aaaatcacac agtgatcaca aattttaaac agagcacaaa atgctgcctc gaaatgaggg
     7321 cgggaaaata aggttatcag ccttgttttc tccctcatta cttgaaggat atgaacctaa
     7381 aacccttttt tataaagcat ttgtccgaat tcggacataa tcaaaaaagc ttaattacga
     7441 tcaatttgat ctacatctct ttaaccaaca atatgtaaga tctcaactat cgcatccgtg
     7501 gattaattca attataactt ctctctaacg ctgtgtatcg taacggtaac actgtagagg
     7561 ggagcacatt gatgagcacg tcagacgata ttcataacac cacagccact ggcaaatgcc
     7621 cgttccatca gggcggtcac gaccagagcg cgggggcggg cacaaccact cgcgactggt
     7681 ggccaaatca actccgtgtt gacctgttaa accaacattc taatcgttct aacccactgg
     7741 gtgaggactt tgactaccgc aaagaattca gcaaattaga ttactacggc ctgaaaaaag
     7801 atctgaaagc cctgctgaca gaatctcagc cgtggtggcc tgccgactgg ggcagttacg
     7861 ccggtctgtt tattcgtatg gcctggcacg gcgcaggtac ttaccgttca atcgatggac
     7921 gcggcggcgc gggtcgtggt cagcaacgtt ttgcaccgct gaactcctgg ccggataacg
     7981 taagcctcga taaagcgcgt cgcctgttgt ggccaatcaa acagaaatat ggtcagaaaa
     8041 tctcctgggc cgacctgttt atcctcgcag gtaacgtggc gctggaaaac tccggcttcc
     8101 gtaccttcgg ttttggtgcc ggtcgtgaag acgtctggga accggatctg gatgttaact
     8161 ggggtgatga aaaagcctgg ctgactcacc gtcatccgga agcgctggcg aaagcaccgc
     8221 tgggtgcaac cgagatgggc ctgatttacg ttaacccgga aggcccggat cacagcggcg
     8281 aaccgctttc tgcggcagca gctatccgcg cgaccttcgg caacatgggc atgaacgacg
     8341 aagaaaccgt ggcgctgatt gcgggtggtc atacgctggg taaaacccac ggtgccggtc
     8401 cgacatcaaa cgtaggtcct gatccagaag ctgcaccgat tgaagaacaa ggtttaggct
     8461 gggcgagcac ttacggcagc ggcgttggcg cagatgccat tacctctggt ctggaagtag
     8521 tctggaccca gacgccgacc cagtggagca actatttctt cgagaatctg ttcaagtatg
     8581 agtgggtaca gacccgcagt ccggctggag caatccagtt cgaagcggta gacgcaccgg
     8641 aaattatccc ggatccgttc gatccgtcga agaaacgtaa accgacaatg ctggtgaccg
     8701 acctgacgct gcgttttgat cctgagttcg agaagatctc tcgtcgtttc ctcaacgatc
     8761 cgcaggcgtt caacgaagcc tttgcccgtg cctggttcaa actgacgcac agggatatgg
     8821 ggccgaaatc tcgctacatc gggccggaag tgccgaaaga agatctgatc tggcaagatc
     8881 cgctgccgca gccgatctac aacccgaccg agcaggacat tatcgatctg aaattcgcga
     8941 ttgcggattc tggtctgtct gttagtgagc tggtatcggt ggcctgggct tctgcttcta
     9001 ccttccgtgg tggcgacaaa cgcggtggtg ccaacggtgc gcgtctggca ttaatgccgc
     9061 agcgcgactg ggatgtgaac gccgcagccg ttcgtgctct gcctgttctg gagaaaatcc
     9121 agaaagagtc tggtaaagcc tcgctggcgg atatcatcgt gctggctggt gtggttggtg
     9181 ttgagaaagc cgcaagcgcc gcaggtttga gcattcatgt accgtttgcg ccgggccgcg
     9241 ttgatgcgcg tcaggatcag actgacattg agatgttcga gctgctggag ccaattgctg
     9301 acggtttccg taactatcgc gctcgtctgg atgtttccac caccgagtca ctgctgatcg
     9361 acaaagcaca gcaactgacg ctgaccgcgc cggaaatgac tgcgctggtg ggcgggatgc
     9421 gtgtactggg tgccaacttc gatggcagca aaaacggcgt cttcactgac cgcgttggcg
     9481 tattgagcaa tgacttcttc gtgaacttgc tggatatgcg ttacgagtgg aaagcgactg
     9541 acgaatcgaa agagctgttc gaaggtcgtg accgtgaaac cggcgaagtg aaatacacag
     9601 ccagccgtgc ggatctggta tttggttcta actctgtcct gcgtgcggta gcggaagttt
     9661 acgccagcag cgatgcccac gagaagtttg ttaaagactt cgtggcggca tgggtgaaag
     9721 tgatgaacct cgaccgtttc gacctgctgt aatctgacct tcttcagcga ctgtctttca
     9781 ggcagtcgct gaagtttctt taccggcgta tagtgtccac aggaaaacta cacactggat
     9841 ctctcatgtc tgccgcagga aagagcaacc cactggcaat cagtggcctg gttgtgctca
     9901 cacttatctg gagttatagc tggattttca tgaagcaagt caccagctac atcggtgcct
     9961 tcgactttac cgccttacgc tgcattttcg gcgctctcgt tttattcatc gtccttttat
    10021 tacgtggtcg cggaatgcgc ccgacaccgt ttaaatacac cttagccatt gccctgttac
    10081 aaacctgcgg gatggttggc ctggcgcagt gggcgctggt cagcggcgga gcggggaagg
    10141 tggcgatcct aagctatacc atgccgttct gggtggtgat tttcgccgcg ttgtttctcg
    10201 gtgaacgcct gcgacgtggg caatatttcg cgatactgat tgccgctttc ggcttatttt
    10261 tggtgttgca gccgtggcaa ctcgatttct cttcaatgaa aagtgccatg ctggcaatcc
    10321 tctccggcgt cagttggggg gcgagcgcga ttgttgctaa acgcctatac acccgtcatc
    10381 cgcgcgtgga tttattatcg ttaacatcct ggcagatgct ctatgcggcg ctggtgatga
    10441 gtgtggtcgc tttactggtg ccgcaacgtg aaattgactg gcagcccacc gtgttctggg
    10501 cgctggccta cagtgcgatt ctggcgacgg cgctggcgtg gagcttatgg ttgtttgtat
    10561 tgaaaaactt gcctgccagt attgccagct taagcacgct ggccgttccc gtttgcggcg
    10621 tactcttttc ctggtggctg ctcggtgaga atccgggggc cgttgaaggt agcggtattg
    10681 tgctgattgt gctggcactg gcgctggtga gccgtaagaa aaaagaagct gtcagtgtaa
    10741 aaagcatctg aattttttct tcatgtgggg cgatcgctta tttaacaaaa taacgataat
    10801 gccccaccat ccgccagcta aacagcacat cttcttcctg cgcgcctgcg ccaatgttat
    10861 gtatcaccag cggcgtaccg tcgcgggcga agccatctga caccacccca atatgtgcca
    10921 gtccgttatc cagtcgccag gagacaatat cgcccgcttg atagtcactg gggttcttgc
    10981 tgatggggcg tgttttatcg tggcgggtaa accaggtttc cagattaggc acccgacggt
    11041 gatcgatgtt gctgtccggg cgctttaact gccatttttg cgggtactcg gcaaaattct
    11101 tcgccatatc ttcgtgcacc agtttctgca aatcgacctt ctggctgcgc aatgcacgga
    11161 tcaccacatc ggaacatacc ccgcgttctt gcgggacatc accgccagga taggtaagct
    11221 gcatatacgc cggatcgtaa aatagcgtgc tgccgatttg ctgtctggca ccgtctgcga
    11281 tggcaaggtt ggtattggcc tggatttgta ccacggttgg tggaacggcg ggagatttta
    11341 aggagtggct ggtaaatgcc gttagcaggc tgagcagcgc cagtgaagct ttcatctggt
    11401 tcgtccgtga aaataagagt cacagattcg accttccggg ctaacgattg ttctgtggag
    11461 aaaatgtcca gaagcggata aaaaactcct gaacaacgtg ggagtttcag attactggca
    11521 cagagtgccg gatgcggagt gaacgcctta tccggcctac aaaagcacgc atattcaata
    11581 tattgcagag atttcgtagg cttgataaac gtagcgcatc aggcaatttt gcgttcaaac
    11641 tcccggacaa gccgggagtt tggagtaggt tattcccact cttgcaggaa acgctgaccg
    11701 tactggtcgg ctaccagcag agcggcgtaa acctgatctg gcgtcgcgcc gccaggcatg
    11761 ttgtgaatgg tttcaccttc tgcacatgcc gcttctgcca caattcgcat tttcgccggg
    11821 acatcttctt taatatccag ttgagcgaga gttattggca aacctaccgc atggctaagt
    11881 gcagctacgg tttcgatttc ctcaaccggt gcgttttcca gaaccagctg cgtcagcgta
    11941 ccaaatgcca ctttttcacc gtgataatag tgatgcgcgt ccgggatagc ggtcaggccg
    12001 ttatgcaccg cgtgagccgc cgccagcccg ccgctttcaa aaccaacgcc gctcaaatag
    12061 gtgttcgctt caatcacgcg ctccagcgcc ggggttacta catgctgttc ggcggcaagc
    12121 atcgcttttt cgccttcttc cagcagggtg ttgtagcaca gttcagccag tgccagcgcc
    12181 gcctgggtgc acttgccgcc cgccatggtg gtcgcgccgc tacgagagca agcacgcgct
    12241 tcaaaccagg ttgccagcgc atcgccgata cccgctgcta acagacgtgc aggtgcgccc
    12301 gcgacgattt tggtgtcgac aatgaccata ttcgggttat ttggcaacag cagatagcgg
    12361 tcaaactcac cctcatcggt gtagataaca gacaatgcgc tgcacggtgc atcggtagag
    12421 gcgatagtcg gtgcgatcgc taccggaaca cccatgaaat gtgccagtgc tttggcagta
    12481 tcgagggttt ttccgccacc gataccgaga attgcgccac actgcgcagt ctccgcgatg
    12541 ccacgcagac ggtcgatctc attttgcgaa cattcaccgc caaacggcgc aatttctact
    12601 accagtccag catctttaaa gcttttctcg acagtggatt gagcaaaacc taaaacaaat
    12661 ttgtcaccca ccactaacca gcgttctgcc agcggcttca ggtattcgcc cagacgatta
    12721 atcacatcag cgccctggat gtatttaccc ggtgattgaa taatgcggtc cataattgct
    12781 cctttagaga tgagtagtgc caaatgcggc attccagtcg tgttcgaact tctctatagc
    12841 tgactctacc gcaggggtgt tgagcatttg ttgcgctaca tctaagggca gggtgatgga
    12901 ttcacatcct gccagtaaac agtccagcgc ctgacgcggc gttttaaagc tggccgccag
    12961 caccatgctt tctggtgcgt gcatttctaa cagcgcttgc agctcctgaa ccgtacgaat
    13021 gccgtccccg ccctgggcat ctacgcggtt aacatacgga gcaacgtatt ttgcccctgc
    13081 cagcgcggcc agtaaccctt gtgcagcact gtacactgcg gtgccaagcg tagtaatacc
    13141 ctcttttttc agcatcttaa tggcggccag accttcggaa gtcaccggga ttttcaccac
    13201 aatgcccgga atggcgtcgc gcaggcgctt cgcttcttcc actataccct gcgcgtcacg
    13261 actcatagtc tgagcaaaca gaatgccctc atcaccaatc gctttttgca gacgcggcag
    13321 aacttcccat atggactcct tgctggcagc gataatgctc gggttagttg tcaccccggc
    13381 aatggggaat atgcgtgcca gacgttcgac ttctgcgacg ttagcggtgt ccagatacag
    13441 ttccatgatg tttcctttta cagttccagt tcatgttgca gcaggctggc gatagcgtcg
    13501 gcagaggcgg cgttaaccag cgcgttacgg aattcttcgt gcatgatgcg gcgagcgagg
    13561 cgcgagaaaa tgcgcatgtg ctgatcgccc gcagcgtgtt tgttcagggt taacataatg
    13621 atgaattgcg cttcatcatc gccccagcgc accggcgctt gcagacgcgc cacgctgatg
    13681 gtggattgct caatgtgttc tgatttgctg tgtggaatgg caaaactaaa gcccagaccg
    13741 gtagagaaaa cggcctcgcg cgcccataag tcggcttcca gtttacgcgg atagcggcag
    13801 cggcccgcca gcagcaggtt atcggtcatg cctttgagca cttcttcttt gctgcgccag
    13861 tcactttcca gcgtgatgca ctcggcggtg accagcggtg cgtcctgttg ggtcatgcgg
    13921 aattgcgcca gcaggtgttc cacttccagc gaagtacggc aggccattgc ctggttgagc
    13981 aacttgcggc actcacggct atcaagttgc gccatccgcg ctttcgccgc cggaattgat
    14041 ggtgcgctca tgctgagttc atccagccct aagccgacca gcaacggcag cacggaacct
    14101 ttcgctccca gctcaccgca cagaccaatc catttgccct ggcgatgcac cgcctgcacg
    14161 gcgtaatcga gcgcccgcaa gaatgccgga ttcaggctgt tgtagtgacg agtaacctta
    14221 gcgttatcgc gatccactgc cagcagatat tgcgtcaggt cattactacc aatactaaag
    14281 aaatcaatct cttcgcagca ttgatcgatg atgaacatca ccgacggtac ttccagcata
    14341 atgccaagct ggatcttctc atcaaacgga atgtgttcgt tacgcagttg ctgtttggct
    14401 tccgccagtt tttctttcac ccataagatc tcttccattg aggagatcat cgggatcatg
    14461 attttcaggc tgccgtgagc ggaggcgcgc aggatcgacc gtagttgtgt ggtgaacagc
    14521 gaggcgtatt cttcataaat acgcacagcg cgataaccga ggaacgggtt agcttctgcc
    14581 ggaatgttca gataatcaac gggtttgtcg ccgccaatgt ccatagtgcg cacaataatg
    14641 ctgcgtccgt tggcagactc cagcgcctga caaaaaatgt tgtacagctc gctttcgccc
    14701 ggtgcactgg tgcgatccat atagagcatt tcagtgcgga acaaaccaac gccttccgca
    14761 ccattgccga atgcggcctg cgcttccacg gagtgagcga tgttagcggc aatttcaatg
    14821 cggataccgt cagcggtacg ggcttgttgg gtcagccaga cacgctgttg ctcacgcagg
    14881 gcgtcctgta cgcgggcttc ttgctgataa taacgagcta cggcttctcc tggctcaacc
    14941 acaatcgccc cagcgttgcc gtcgatataa atcgtttgat gctgccacgg agtaagggca
    15001 tcaatatcta caccaaccag tgtcggaatg ttgaacgaac gggcaagaat tacggtatgt
    15061 gaggtggtgc cgccactttt cagtaacaga cctttgaggt gatttttatc cagttcgagg
    15121 aactggctgg gagtcagttc atcagccata caaatggcgg gctgcgtcag tttgcccggt
    15181 gccgggaagc gttgctcacc gtagatttgc tggagtaact ggaagcagac gtcgcgtacg
    15241 tccagggcac gttcttgcag gtagctgctg ctggaacggg cgaactcttc gcaaaagtga
    15301 ttcgcactgg tgacaatcgc ttcggcgcag cttaatccgg cgctgacgcc tgccagtaaa
    15361 tgttcgcgca gggaagtatc gccagccagg gatcggtgcg cttccagaat cgcgctggtg
    15421 gcaccgtcgc tatccagcag acgaaactca atgtttttca gtaccagcgt caggccgttt
    15481 tccagtgcgg attgctcggc gtcaacgcct ttggctgcgg gaagattacc cagcgcattg
    15541 agatctaaag aagagatcgg cgtcagaatg ccgcctgcac taccgctgca cacggtgcgg
    15601 gcgcggataa tttgcggatt cagattggtc agtgaaaccg gcagtggttc cagttcgtca
    15661 gatttaactt ccgccagcgg cgcatcgcag tgggggaatt catcgcgcag ccattggctt
    15721 aaacgctggt gcgcttcctg ttcgtcggcc ccggaaatca gtaactggca gttatcgccc
    15781 gccagcgtat cggtgccaat cagcgcaagg gcgcttttgg cgttgccctt gcggtcagtg
    15841 cgcaggttat gccactcaat ttgtgatgaa aaagtattac acagcgtttc aacgtggctt
    15901 gccggacgtg catgtacgcc gttaggtagc tcacaaataa attccacaat cagggccatt
    15961 gcctctctcc cggtacggtt ttctgtttac aggataagag cgcacggcaa cggcctgcca
    16021 tgtgacaaat ctgccaaaag ctggacaaat gtaatgtaac cgtcaatttg cgacgcgtct
    16081 cacaagacgc tgttttgcgg catgcttccg gtttatcgca agttatgagg cggatcgcat
    16141 ttttgtactg atattacaaa agtccagtaa atggcctttt tatccactgt ttgctccgcc
    16201 tgcgattgcc tattgtttcc tccatcaaaa atatggacat gggctttcgc aagcccggga
    16261 gcagcttatg aatgagttgg tgcagatcct gaaaaatacc cgtcagcatt taatgacggg
    16321 cgtttcacac atgattccct tcgtggtatc gggcggtatt ttgctggcgg tttccgtcat
    16381 gttgtatggc aaaggcgcag tgccggatgc cgtagccgat ccaaatctga aaaaactgtt
    16441 tgatatcggc gttgcgggct tgacgctgat ggtgcctttc ctcgcagcgt acatcggtta
    16501 ttccattgca gagcgttctg cgctggctcc gtgcgctatc ggtgcctggg ttggtaacag
    16561 ctttggtgcg ggcttctttg gtgcactgat cgccgggatt atcggcggca tcgtggtgca
    16621 ttacctgaag aaaattccgg tgcataaagt tctgcgctcg gtgatgccta tcttcattat
    16681 tcctatcgtc ggcacactga ttaccgcagg catcatgatg tggggcttgg gcgagcctgt
    16741 aggggcgttg accaacagcc tgactcagtg gcttcagggg atgcagcagg gcagcattgt
    16801 tatgctggcg gtgatcatgg gtctgatgct ggcgttcgat atgggcggtc cggttaacaa
    16861 agtggcctat gccttcatgc tgatttgcgt tgctcagggt gtttataccg tggtggccat
    16921 cgctgccgtg ggtatttgta tcccgccgct ggggatgggg ctggcgacgc tgattggtcg
    16981 taaaaatttc tccgcagaag agcgcgaaac cggcaaagcg gcactggtga tgggctgtgt
    17041 tggcgttact gaaggggcga ttcctttcgc cgctgccgat ccgctgcgtg tcattccttc
    17101 catcatggtc ggttcagttt gtggtgcagt aactgcggcg ctggtcggtg cgcagtgcta
    17161 tgcaggctgg ggtggtctga ttgtgctgcc ggtggttgaa ggcaagctgg gttatatcgc
    17221 agcagtggct gtcggagcag tggtgacggc tgtttgtgtg aacgtgctga aaagtctggc
    17281 gcgtaaaaat gggtcttcga ctgatgaaaa agaagacgac ctggatttgg attttgaaat
    17341 taattaattg aggaacgagc catgacgaaa attattgcag taaccgcatg tccttcaggt
    17401 gttgcccata cctatatggc agcagaggcg ctggaaagtg ccgcgaaagc gaaaggctgg
    17461 gaagtgaaag tagaaaccca ggggtcaatt ggtctggaaa atgaactgac tgcggaagat
    17521 gtggcgagcg ctgatatggt gattctgacc aaagatatcg gcatcaagtt tgaagagcgt
    17581 tttgctggca agaccatcgt gcgcgtcaac atcagcgatg cagtaaaacg tgctgacgcc
    17641 attatgagca aaattgaagc tcacctggcg caaaccgctt aagttcttct cccgctcgca
    17701 agggcgggtt cgctttccca caggagttcc tcatgacgaa tcgtatctct cgcctcaaaa
    17761 ctgcactgtt tgccaatacc cgtgaaatct cgctggagcg ggcgctgctt tataccgcca
    17821 gccatcggca aaccgaaggc gaaccggtga tattgcgccg ggcgaaagca acagcgtata
    17881 tccttgaaca tgttgaaatt tcgattcgtg atgaagaact gattgccggt aaccgcaccg
    17941 taaaaccgcg cgctgggatt atgtcgccgg aaatggaccc ttactggctg ctgaaagagc
    18001 tggatcaatt cccgacgcgt ccgcaggacc gctttgctat cagtgaagaa gataaacgta
    18061 tctaccgtga agagttgttc ccgtactggg aaaaacgttc gatgaaagat ttcatcaacg
    18121 ggcagatgac agatgaagta aaagccgcga ccagcacgca gattttcagc atcaaccaga
    18181 cagataaagg ccaggggcac attattattg attacccacg cctgctgaat cacgggctgg
    18241 gggagctggt ggcgcagatg cagcaacatt gtcagcaaca gccggagaat cacttttatc
    18301 aggccgcgct gttactgctg gaagcctcgc agaaacacat tctgcgttac gctgaactgg
    18361 cggaaacaat ggcggcaaac tgcacagatg cccagcgtcg cgaagaactg ctgactattg
    18421 cggagatctc ccgccataac gccgaacata agccgcagac gttctggcag gcgtgccagt
    18481 tattctggta catgaacatc attctgcaat acgaatccaa cgccagttcg ctatcgttgg
    18541 ggcgcttcga ccagtatatg ttgccgttct atcaggcatc tttaacccag ggcgaagatc
    18601 cggcgttcct gaaagaacta ctcgaatctt tatgggtgaa atgcaacgac atcgtgctgt
    18661 tgcgctccac cagcagcgcg cgttatttcg caggtttccc gaccggctat accgcactgc
    18721 tcggcgggtt aaccgagaac ggacgtagcg cggtgaacgt gctttcgttc ctttgccttg
    18781 acgcttatca aagcgtgcaa ttaccgcaac cgaacctcgg cgtgcgcact aacgccttga
    18841 tcgacacgcc gttcctgatg aaaaccgccg aaaccattcg cctcggcacc ggtattccgc
    18901 aaatctttaa cgatgaagtg gtggtaccag cgttcctcaa ccgtggcgtt tcgctggaag
    18961 atgcgcgcga ctattccgta gtgggctgtg tggaattatc tattcccggc agaacctacg
    19021 gcttgcatga catcgcgatg tttaatctgc tgaaagtgat ggaaatctgc ctgcatgaaa
    19081 atgaaggcaa tgctgcgctg acttatgaag gtttactgga gcagatccgc gccaagatca
    19141 gccactacat caccctgatg gttgagggca gcaatatttg tgatatcggc catcgcgact
    19201 gggcacctgt accgctgctc tcgtctttta tcagcgattg tctggaaaaa ggtcgcgata
    19261 ttaccgatgg cggcgcgcgt tataacttct ccggcgtaca ggggatcggt atcgccaacc
    19321 tgagcgattc tctccatgcg ttgaaaggga tggtttttga gcaacagcgt ttaagttttg
    19381 acgaattgct gtcggtatta aaagccaact ttgcaacgcc agaaggcgaa aaagtccgtg
    19441 ctcgcttaat taaccgcttc gagaaatacg gtaacgatat cgacgaggtg gataacatca
    19501 gcgccgaact gttacgccac tactgcaaag aagtggaaaa ataccagaac ccgcgcggcg
    19561 gttacttcac gccgggatcg tataccgttt ctgctcacgt cccgttggga tcggtggttg
    19621 gcgcgacgcc agacggtcgt tttgccgggg aacaactggc agacggcggc ttgtcaccta
    19681 tgctgggcca ggacgcacaa gggccaacag cggtactgaa gtcagtcagt aagctcgata
    19741 acacgctgct gtctaacggt acgttgctga acgtgaaatt cactccggcg accctggaag
    19801 gtgaagcggg attacgcaaa ctggccgact tcttacgggc gtttacccag cttaagttgc
    19861 agcatattca gtttaacgtg gtgaacgccg acacgttgcg ggaagcgcaa cagcgcccac
    19921 aagattatgc cgggctggtg gtgcgcgttg ccggatacag cgccttcttt gtcgaactgt
    19981 cgaaggagat ccaggatgac atcatccgcc ggacagcgca tcagctgtaa cgttgtggaa
    20041 acgcgccgcg atgatgtggc gcgcattttc aacattcagc gttattcact gaatgacggc
    20101 gagggcattc gtacggtggt cttttttaaa ggctgtccgc atctttgccc gtggtgtgct
    20161 aatccggagt cgatctccgg caaaatccag acggtacgca gagaggcgaa atgtctgcac
    20221 tgtgcgaaat gtttgcgtga tgcggatgaa tgcccctccg gtgcgtttga acggattggt
    20281 cgcgatatca gccttgacgc tctggaacgg gaagtgatga aagatgacat tttctttcgc
    20341 acgtccggcg gcggcgtcac gctttctggc ggcgaagtgt taatgcaggc cgagtttgct
    20401 acccgttttt tacagcgact gcggctgtgg ggcgtgtcat gcgccatcga aactgccgga
    20461 gacgcgccag ccagcaagct gttaccgctg gcgaaattgt gcgatgaagt gttgttcgat
    20521 ttaaaaatta tggacgcgac tcaggcgcgg gatgtggtga agatgaacct gccacgcgtg
    20581 ctggagaatc tgcgtttgct ggtgagtgaa ggtgtcaacg tgatcccgcg tttgccgctg
    20641 atccccggtt tcacactcag ccgggagaat atgcagcagg cgctggacgt gctgatcccg
    20701 ctgaatatca ggcagatcca tctgttaccg tttcatcagt acggcgaacc gaaataccgt
    20761 ctgctgggga aaacatggtc gatgaaagag gtttctgcgc cgtcgtcagc cgatgtggca
    20821 acgatgcgcg aaatggcaga acgggccgga tttcaggtta ccgtgggagg ttaaaatggc
    20881 atacctggtg gcagtaaccg cctgcgtcag tggcgtggcg catacttata tggcggcgga
    20941 acggctggaa aagttgtgcc agttagagaa gtggggcgtc agcattgaaa ctcagggcgc
    21001 gctgggaacg gagaatcgtt tagcggacga ggatatccgc cgggcggatg ttgcgctgtt
    21061 gattacggat atcgagcttg ctggtgccga gcgatttgaa cattgtcgct acgtgcaatg
    21121 cagtatctac gcgttcctgc gtgagccgca gcgggtgatg agcgcggtac gtaaagtgct
    21181 ttctgcgccg cagcaaaccc atcttattct ggagtagtcg gtttttctgt cagttggctg
    21241 tggtactgcc gacgatattc cgacggcgag cgttcggtgt ttttacgaaa cagacggcag
    21301 aagtagttgc tgtcgacaaa gccgcaggcg tgcgccactt cttttacctt caggtcatag
    21361 cctttcaata acgtcttagc gtgctccagt cgcgtgtgat tcaggtattc gttaaaacca
    21421 atggcccccg tcttttgaaa caggtgcgag agatagtttg gtgaaatata gaacgcctgt
    21481 gcgacagatt cgcgggtaag cgcggaggca tagcgttcgt cgatataatc acgaatagct
    21541 tcgaatagag cctggctgcg tgaggcggtc tgtatttggc tgccgagcaa atcgcggcag
    21601 tggctaagca ggctggcgac gataagcctt gccgtttgct gctcctgtgg ctgcatttgc
    21661 atttcattga gcgtttgtag cagaaaagaa ccaatgcgcg ggccgcgtcg ggcgacgtgt
    21721 tgcttcgcca gattttgata ctgtttgcca tcccattgca cgacgctgaa gccgagttgc
    21781 tgtttgccaa acagcacgct aaaggtagta gcgggggctt gccattgtgg aaaattccag
    21841 ccaccagcgg gaacgtacag cacatcgccg ggaactaacg ttgctccagc gccggtatca
    21901 acaaactcac cttccagcac tatttccagc cgtggaaaat cgacctgata cgcaagatcg
    21961 ggcgccggac cgttactgct ggcaaagtaa atctggcgca gggacagcgg gccgttgatc
    22021 aggcgggaga gcagatagct gacgtcgtga tacatgttat tcccttaggt atgtagacgg
    22081 gaaaacagta actggcatta ttaaaaaagg ccacctgaaa agtgtggcct gaggggagtt
    22141 cgatgtaaac attgcggcag tatcgcatcc ggcaaatagc gccagacgcg atacaaaggt
    22201 catttactga ttacccacct gatcgccata cggcagcgtg tcgtaatcga tcagcgcgtt
    22261 tttcttgtac gggttaccaa tccagcgggt agtttctttg aactgcggat tcaccactga
    22321 acgggttgga tcgtaaccgt cgtaagataa gcctgctaaa tctgaccagg tgtggatcag
    22381 ttccgccagg ctgtatttac gatcaacatc ctgcgagaaa tcacgcggat gagtcgcttg
    22441 ccatttttcc gacgtccaca gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc
    22501 ttcgttacgc ccctgagttt tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga
    22561 gaaataaacc aggaaaccgt tcgggtttgc tgctttaaag tctttaatca ggctggcaac
    22621 cacatgatcg ttatacaggt tagcgttgtc ataatcgtta tatgactcca gctcttccgc
    22681 gtttaatcca ggcggaacat gatcggtatt gccatcaaac ttgccctgat tttccgggta
    22741 gcggtatttg tatttgatat gcgtacccag cagatgaacg atgatcagtt tcttcggcgc
    22801 cgggtccttc agcacttcct ggaatggttt cagcacgttg gtgtcgtatt cacgcgcact
    22861 ctgcgtacgt tgctggttca tatagaactg tttgtcggtc tgcttcgaaa ataccgtcag
    22921 catggtattg cgggcggtca tcgtctgctg gttggtgatc cagaaggttt tataacctgc
    22981 ctgtttcatc atgttcatca gcgacggctg cgtcagatac agatccgggt tcttttcatt
    23041 ggcaaaggtc agcgcctgtt gcaggatttc aatggtgtac ggacgagacg taactacgtt
    23101 attaaacacg gtcagattcg gatcggtttt atgcagcgca tccagctccg gcgtggtttc
    23161 acgcggataa ccgtacagac tcatgcgtcc gcgctgggtc gactcgccaa tcaccagcac
    23221 taaagtgcgc ggttcgttac ccgattcatc tttgaaatta gccagtggcg gcaaggcatt
    23281 attttcattc agtaactttg ttagcgagtt tagttgctga cgatactgat aatagccggt
    23341 caggaattgc cacggtgcgg caggctccat acgcgaggcc aggttatcca acgttttctc
    23401 aaacggcttg tttttgataa acgtattcat ggcgatcgga tgcagaatca agccataaag
    23461 cagggcaaaa gagacaacat aacgccacgg ctttggaata tagaccgggc gcaggcgtgt
    23521 ccacagcaga actgccaccg ccgtataggc cagcgcgata agcacaattt tcaggctgaa
    23581 atactggctt aaatactcgc tggcttcgtt ggtgttggtt tcgaacatca caaacagaac
    23641 gctctgcgag aactcctgcc cgtagatgac gtagtagcat agcgccgcca gagaggccgc
    23701 ccatagcacc acgccgatta ctgcggcaat aattttaatc cgcttcggaa agaggaatac
    23761 cgggatcaac cacagcgagc tgaataacag cgagtcgcga atgccgttgg tgccactata
    23821 accactgatg taaataatgg cctgtagcag agtagagaaa aaccaaaagt agagcagtgc
    23881 ccaacccagg gctttccagc taaaaagggg tttagcctgg acttctgtgg aatgcatagt
    23941 aagaacctgt cttgaaaaaa atatcgccga atgtaacgac aattccttaa ggatatctga
    24001 aggtatattc agaatttgaa taaaatgcag acagaaatat attgaaaacg agggtgttag
    24061 aacagaagta tttcagaaaa ccctcgcgca aaagcacgag ggtttgcaga agaggaagat
    24121 tagccggtat tacgcatacc tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg
    24181 acgcgaggat ccggttcctg gccttctttt tctgcctggc gggagcggtg cagtaactcg
    24241 gcctgcaata cgttcagcgg gtcggtgtaa atattccgta gctgaataga ctctgcaatc
    24301 cacggcagat cggccattag atgggaatcg ttggcaatcg ccagcaccac tttgatgtct
    24361 tcttcttgca ggttgcgtaa ctctttacct aacggccaca gtgctttgtc taccaggcgt
    24421 tggtcatagt attccgccag ccacaggtct gctttggcga agaccatctc cagcatgccg
    24481 agacgcgtcg agaagaatgg ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca
    24541 tcttcgacca ctttttgcag cgccgtacct gcacccagcc aggcggggag catcaggcgg
    24601 ttttgcgtcc aggcaaaaat ccacggtatg gcgcgcagcg actcgacgcc gccggttggg
    24661 cgacgtttcg ccggacgtga acccaacggc agtttgccca attcttgttc cggcgtagcg
    24721 gagcggaagt aaggcacaaa atctttgttt tcacgtacgt agccacggta gagatcgcag
    24781 gagatgactg acagttcatc cataatgcga cgccagctct ctttcggctc aggtggtggc
    24841 agcaggttgg cttccagaat cgccccggtg tacagcgaca ggctgctgac ggtgatttct
    24901 ggcagaccat atttaaagcg gatcatctcg ccctgttcag tcacgcgcag gccacctttc
    24961 aggcttcctg gcggttgtga cagcagcgcc gcatgagcag gtgcgccgcc gcgaccaatg
    25021 gaaccgccgc gaccgtggaa caacgtcagc tcaatacccg ctttttcgca ggttttgatt
    25081 aatgcatcct gtgcctgata ttgcgcccag gaagctgcca tcactcccgc atcttttgct
    25141 gagtcggaat agccaatcat caccatctgt ttgccctgaa tcaggccacg ataccagtca
    25201 atattgagca gctgggtcat gacatcgttg gcgttgttca gatcatcgag ggtttcaaac
    25261 agcggagcaa ccggcatcgc aaacccgata cccgcttctt tcagcagcag gtggacagcc
    25321 agtacgtcgg acggcgtttt cgccatcgag atcacgtagg cggcaatgga gccttgcggc
    25381 gcttcggcaa tcacctggca ggtatcgagc acttcgcgcg tttcggcgct tggttgccag
    25441 ttgcgcggca gaagcggacg tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg
    25501 gcctctgacc agctttcgta gtcgccgata ccgaggtagc gggtcagctc gcccagcgct
    25561 tcggtatggc gggtgctctc ctgacggata tcaatacgga ccagcggtac gccgaaacat
    25621 ttcacgcggc gcagggtgtc gagcagatcg ccgttggcga taatacccat gccacacgcc
    25681 tgaagtgact ggtagcaagc gtagagcggt tcccacagtt cttcgttttg tgtcagcagc
    25741 ccttctggtt ttggcagttc ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc
    25801 atcaggcgag aacgcaggtt tttcatcaga tagcgatacg gttctgcggc accttcttcg
    25861 ccaaccagcg ccagcagttc aggggtcgct tcaaccatcg acagttcaga aaccagcacc
    25921 tgaatatctt tcaggaacaa atcggtggct ttccagcggc tgagtagcag gacgtggcgg
    25981 gtgatatcgg cagtgacgtt cgggttgccg tcgcggtcgc cgcccatcca cgaagtaaaa
    26041 cggaccggaa caaattcgac gggcagtttg tagccgaggt tctcttccag ttgttcgttc
    26101 agttcgcgca ggtaatttgg tacgccttgc cacaggctgt tttccactac ggcaaagccc
    26161 catttggctt catctaccgg gcttggacgc agcttacgga tttcatcggt atgccatgac
    26221 tgggcgatta actggcgcag gcgacgcatc agctggttgt gttcgtagtc agcgatatct
    26281 ttgttatcga gctgttttaa gcaggcgttc acttccacca ttttgtggat cagtgtacga
    26341 cgggtaattt cggtagggtg agccgtgagg accagttcca gcgacagcga ttccactgct
    26401 tttttgatgg tgtcttcgct cagttccggc tggtttttca gtttacgcag ggtgcgggcg
    26461 atcacttccg ggttgctggc agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg
    26521 gtgttggcca ggttcaggaa ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc
    26581 gacaaatttt gtaaggtggt gagtaactcc tggcggttag catcattgcc agcgcgtgaa
    26641 gatttggaca acttacggat agtttctacg cgttcaagaa tgtgttctcc caacgcatcc
    26701 ttgatggttt ctcccagcac tttgccgagc atactgacat tactacgcaa tgcggaatat
    26761 tgttcgttca tattacccca gacaccccat cttatcgttt gatagccctg tatccttcac
    26821 gtcgcattgg cgcgaatatg ctcgggcttt gcttttcgtc gtcttttata aagccacgta
    26881 aaagcggtga cgtcaaatgc tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt
    26941 acgtcattaa attcacgacg ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc
    27001 cccaagtaac accaaaggtg taggtcggat aagatgcgca agtatcgcat ccgacattat
    27061 tgcggcactg gagtttgaca acagtgccgg atgcggcgcg agcgccttat ccggcctacg
    27121 ttttaatgcc agcaaaaatg gtgaattacc tgggttatca gttcgcgggt gggcttgata
    27181 aaccgtgttt ccagatattc atcaggttga tgagcctgat taattgagcc aggccccaac
    27241 accagcgtcg ggcataacgt ttgaataaac ggcgcttcgg tacagtagtt caccacttcg
    27301 gtctttgctc cgagcaattt ctcaactact tcaaccagtt gatgattcgg tgggcattca
    27361 tagccaggga tcggcggatg cagctcgtcg accgtcagac gacccggcca gcgttcgctc
    27421 accggagcca atgcatcgtt gagcaaacca ttaagttcat tgagtgtcat gccaggcagc
    27481 ggacgaatat ccatatgcaa ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg
    27541 tgaatatgcc cgaggttgag cgtagggtat ggcacggtaa acgcttcgta gtgataacgt
    27601 tctttcaggt tatcgcgcaa ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata
    27661 gcgttaactc cgcgtgctgg atcgctggag tgccccgact ggccctgaat acggatggcg
    27721 ttagagatat gacctttatg tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg
    27781 gcgcaatccg ggcgcagggc ggtagtttcg gcaaaataac gcgctccggc catactggtt
    27841 tcttcatcag cagtcgccag aatgtagagc ggttttttca gtttcgtgac gtcgacatcg
    27901 cgtagcgcat caaggataaa cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa
    27961 agcttgccgt catgctccgt cagtgtaaac ggatcacgcg tccagcgacc gtcatcaaat
    28021 ggtaccgtat cggtatgccc cgccagcaac aagccgccag ccccctgtcc gatacttgcc
    28081 agcatattaa atttgttgcg agttcctggc acaggctgca cttccacatt gaagcctaaa
    28141 tctttaaacc agtccgccag cagagtgatt aaatctgcat tgctttgatc gagtgcctct
    28201 tccgtggcgc ttattgaagg tgtggcaatc agagcacggt aaatctcgat aaatggcggt
    28261 aatttgtttt tcattgttga cacacctctg gtcatgatag tatcaatatt catgcagtat
    28321 ttatgaataa aaatacacta acgttgagcg taataaaacc caccagccgt aaggtgaatg
    28381 ttttacgttt aacctggcaa ccagacataa gaaggtgaat agccccgatg ttgaatacgc
    28441 tgattgtggg tgccagcggc tacgctggcg cagagctagt gacctatgta aatcgccatc
    28501 cgcatatgaa cataaccgct ttgactgttt cagcgcaaag caatgatgcg ggcaagttaa
    28561 tctccgattt gcatccgcag ctaaaaggca tcgttgatct gccgttgcag ccgatgtcgg
    28621 atatcagcga gtttagccca ggggtggacg tagtgtttct cgccaccgcc catgaagtta
    28681 gccacgattt agcgccgcag tttcttgaag cgggctgcgt ggtgttcgac ctttccggcg
    28741 cgtttcgtgt taacgacgcc accttctatg aaaaatatta cggctttacc catcaatacc
    28801 cggaactgtt ggaacaggca gcctacggtc tggcggagtg gtgcggtaat aaattaaaag
    28861 aagcgaattt gattgcggtg ccgggctgtt atccgacggc ggcacagctg gcgctgaaac
    28921 cgttgattga tgccgatctt cttgacctca atcagtggcc ggtgatcaac gccaccagcg
    28981 gcgtgagcgg tgcagggcgt aaagcggcca tttcaaacag cttttgtgaa gttagcctgc
    29041 aaccgtatgg cgtctttact catcgccatc aaccagagat cgccacacac ctcggtgctg
    29101 acgttatctt caccccacat ctgggcaatt tcccgcgcgg cattctcgaa accattacct
    29161 gccgcctgaa atcgggtgtg acccaggcgc aagtcgcgca agtgttacag caggcgtatg
    29221 cccataaacc gctggtgcgg ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg
    29281 ggctgccatt ttgcgatatc gggtttgccg ttcagggcga gcatctgatt attgtggcga
    29341 ccgaagacaa cttactgaaa ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt
    29401 tcggctatgc ggaaacgcag tctcttattt aagggtgcaa tgatgaatcc attaattatc
    29461 aaactgggcg gcgtactgct ggatagtgaa gaggcgctgg aacgtctgtt tagcgcactg
    29521 gtgaattatc gtgagtcaca tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg
    29581 gtggatgagc tgatgaaagg gctgaatctg ccggtgaaaa agaaaaacgg cctgcgggtg
    29641 acgcctgctg atcagataga cattatcacc ggagcactgg cgggaacggc aaataaaacc
    29701 ctgttggcat gggcgaagaa acatcagatt gcggccgtag gtttgtttct cggtgacggc
    29761 gacagcgtca aagtgaccca gcttgatgaa gagttaggtc atgttggact ggcgcagcca
    29821 ggttcgccta agcttatcaa ctccttgctg gagaacggtt atctgccggt ggtcagctcc
    29881 attggcgtaa cagacgaagg gcaactgatg aacgtcaatg ccgaccaggc ggcaacggcg
    29941 ctggcggcaa cgctgggcgc ggatctgatt ttgctctccg acgtcagcgg cattctcgac
    30001 ggcaaagggc aacgcattgc cgaaatgacc gccgcgaaag cagaacaact gattgagcag
    30061 ggcattatta ctgacggcat gatagtgaaa gtgaacgcgg cgctggatgc ggcccgcacg
    30121 ctgggccgtc cggtagatat cgcctcctgg cgtcatgcgg agcagcttcc ggcactgttt
    30181 aacggtatgc cgatgggtac gcggatttta gcttaagttt tgttggccgg aggcgcagct
    30241 ttccggcatt gaatttcaaa ataaggaaac agagttatgg cactttgggg cgggcgtttt
    30301 acccaggcag cagatcaacg gttcaaacaa ttcaacgact cactgcgctt tgattaccgt
    30361 ctggcggagc aggatattgt tggctctgtg gcctggtcca aagccctggt cacggtaggc
    30421 gtgttaaccg cagaagagca ggcgcaactg gaagaggcgc tgaacgtgtt gctggaagat
    30481 gttcgcgcca ggccacaaca aatccttgaa agcgacgccg aagatatcca tagctgggtg
    30541 gaaggcaaac tgatcgacaa agtgggccag ttaggcaaaa agctgcatac cgggcgtagc
    30601 cgtaatgatc aggtagcgac tgacctgaaa ctgtggtgca aagataccgt tagcgagtta
    30661 ctgacggcta accggcagct gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac
    30721 gcggtaatgc caggttacac tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg
    30781 tgcctggcct atgttgagat gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag
    30841 cgtctggatg tcagcccgct aggctgtggc gcgctggcgg gaacggccta tgaaatcgac
    30901 cgtgaacagt tagcaggctg gctgggcttt gcttcggcga cccgtaacag tctcgacagc
    30961 gtttctgacc gtgaccatgt gttggaactg ctttctgctg ccgctatcgg catggtgcat
    31021 ctgtcgcgtt ttgctgaaga tctgattttc tttaacaccg gcgaagcggg gtttgtggag
    31081 ctttctgacc gcgtgacttc cggttcatca ttaatgccgc agaagaaaaa cccggatgcg
    31141 ctggagctga ttcgcggtaa atgcggtcgg gtgcaggggg cgttaaccgg catgatgatg
    31201 acgctgaaag gtttgccgct ggcttacaac aaagatatgc aggaagacaa agaaggtctg
    31261 ttcgacgcgc tcgatacctg gctggactgc ctgcatatgg cggcgctggt gctggacggc
    31321 attcaggtga aacgtccacg ttgccaggaa gcggctcagc agggttacgc caacgccacc
    31381 gaactggcgg attatctggt ggcgaaaggc gtaccgttcc gcgaggcgca ccatattgtt
    31441 ggtgaagcgg tggtggaagc cattcgtcag ggcaaaccgc tggaagatct gccgctcgac
    31501 gagttgcaga aattcagccc ggtgattgac gaagatgtct atccgattct gtcgctgcaa
    31561 tcgtgcctcg acaagcgtgc ggcaaaaggc ggcgtctcac cgcagcaggt ggcgcaggca
    31621 attgcttttg cgcaggcgcg gttagagtaa tttgtttata ttgctcccct gaaaatgggg
    31681 agcttaccac gcctacaaag tgcgacactt cgtaggcgga taaggcgttt acgccacatc
    31741 aggcagaaat ttgtcagcat tctggtgagc ttaaatttaa cgcccaacca gaaccattat
    31801 ttgtcacatt cctttttatc attgtgtctt ctttaccttc acactcccca tgaaaatcat
    31861 attttcaaat acatcaacca atacatattt taattaattg aatataatta ttttctattg
    31921 ctgtgcccat ttttaatgat taatggagtt tgttatgtga ttatttaacg tgttgtttta
    31981 acgataatgg ttaattattc aggtggtttt tgagggtatt ttttctcctc ggtggaaatt
    32041 tttgttttta atgtgatcac ggttgaataa tctctttttc tctcttgtat ggtagtaggc
    32101 gtaaaattaa gtttgaaacg tctatttaca acacaggaga ttgcaataat gaaaattatt
    32161 gctgcagatg tttttgttac ttgcccaggg cgtaatttcg ttacgcttaa aataaccact
    32221 gaaagcggac tttgtggatt aggagatgcc acgttaaatg gtcgcgaatt gtcggtcgct
    32281 tcttatctga aagatcatct ttgtccacaa ctaattggcc gcgatgccag ccgtatcgaa
    32341 gatatctggc aattctttta taaaggtgct tactggcgac gtggccccgt cacgatgtca
    32401 gccatttctg ccattgatat ggcgttgtgg gatatcaaag ctaaagcggc aaatatgcca
    32461 ttgtatcaac tgcttggtgg tgcttctcgc gaaggcgtga tggtttattg ccacaccacc
    32521 gggcggacga ttgatgaagt ccttgaagac tatgcaaagc accagcagat gggctttaag
    32581 gcgattcgtg tgcagtgcgg tgtaccgggt atgcaaacta cttatggcct ggcgaagggc
    32641 aaaggcctgg cgtatgaacc ggcaaccaaa ggcctgtggc cagaagagca gttgtggtct
    32701 tcagaaaagt atctcgattt tacgccgaag ttatttgaag cggtgcgtaa taagttcggt
    32761 tttaacgaac atctgttgca tgacatgcac catcgcctga cccccatcga agcggcgcgc
    32821 tttgggaaga gcattgagga ttaccgcctg ttctggatgg aagatccgac gcctgcagag
    32881 aaccaggaat gttttcgtct gattcgccag cataccgtga cgccgattgc ggtcggtgaa
    32941 gtgtttaaca gtatctggga ctgcaagcag ttaatcgaag agcagctgat tgactatatt
    33001 cgcacgacga tcacccatgc aggcggcatt accgggatgc ggcggattgc cgattttgca
    33061 tcgctttatc aggtacgtac cggttcgcat ggcccttcag atttatcgcc tgtttgtcat
    33121 gcggcggcgc tgcattttga cctatgggta ccaaattttg gcgttcagga gtacatgggc
    33181 tattccgaac aaatgcttga agttttcccg cacagctggc gcttcgagga aggttatatg
    33241 cacccaggcg atgaaccggg gctgggtatt tctttcgatg agaaactggc ggcgaaatat
    33301 ccgtatgacc cggcgtatct ccccgtcgca cgactggaag atggcacatt gtggaactgg
    33361 taataacggt tcgcttaatt attttctgaa tagtcatcaa atcgattaat tccgctcaag
    33421 gagctaaaaa tggacgttga tgtgtcaacc agtgttgcgg gtaataaacc gcaacggatt
    33481 cgtcgtattc agacagtgac cctggtttta ttatttatgg cggggatcgt taattttctc
    33541 gaccgttcgt cattgagcgt ggcaggtgaa gcgattcgtg gcgagttagg attatcggcg
    33601 acagagtttg gtgttttgct ttctgcattt tctctgtctt atggtttttc acaactacct
    33661 tccggtattt tgctagaccg ctttggccca cgaattgttt taggcgcagg cttaatattc
    33721 tggtcattaa tgcaggcatt aacaggaatg gttaattctt ttagccactt tattttaatg
    33781 cgtatcggtc tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc
    33841 gactggtatg cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt
    33901 atcggtcagg ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg
    33961 acgatgttcg ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg
    34021 tatcgcaacc gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgccg
    34081 gttaaaccgc gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca
    34141 acctggggga tgattttagg tttctctggc gtcaattata ccggttggct ctacatcgcg
    34201 tggttacccg gttatttgca agccgagcaa ggtttcagtc tggcgaaaac cggttgggtg
    34261 gcggcgagtc ctttcctcgc ggcggcagtc gggatgtggg ttaacggtat tgttgtcgat
    34321 cgactggcga aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt
    34381 ttgatgatgt cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agcccaggcg
    34441 gtagcgttta tctcaatggc gctattctgc gtgcatttcg ctggaacgtc tgcatggggg
    34501 ctggtgcagg tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt
    34561 ggcagttttg tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca
    34621 cactcgttta atctggcgtt ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt
    34681 tacttcttta ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg
    34741 ggcctgtaga ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag
    34801 ttattcgagt tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg
    34861 ctccgtttct gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta
    34921 ttctacctat cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc
    34981 ttgagtacct ggtggcattg gccgagcacc gccattttcg gcgtgcggca gattcctgcc
    35041 acgttagcca gccgacgctt agcgggcaaa ttcgtaagct ggaagatgag ttgggcgtga
    35101 tgttgctgga gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtag
    35161 atcaggcgcg taccgtactg cgtgaggtga aagtccttaa agagatggca agccagcagg
    35221 gcgagacgat gtccgggccg ctgcacattg gtttgattcc cacagttgga ccgtacctgc
    35281 taccgcatat tatccctatg ctgcaccaga cctttccaaa gctggaaatg tatctgcatg
    35341 aagcacagac ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc
    35401 tcgcgctggt gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt
    35461 tgctggctat ctatgaagat cacccgtggg cgaaccgcga atgcgtaccg atggccgatc
    35521 tggcagggga aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg
    35581 gtttctgttt tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaga
    35641 cgctgcgcaa catggtggcg gcaggtagcg ggatcacttt actgccagcg ctggctgtgc
    35701 cgccggagcg caaacgcgat ggggttgttt atctgccgtg cattaagccg gaaccacgcc
    35761 gcactattgg cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg
    35821 cagaggccat ccgagcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt
    35881 aaaccgttta acgcagctac ccgataggct tccgccatgg tcgggtagtt aaaggtggtg
    35941 ttgacgaagt actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata
    36001 tgaataattt cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc
    36061 cgatggaaca aaattttcag cgtgcccacg ttcatgccga cgatttgtgc gcgtgccaga
    36121 tgtttaaact gggcgcggcc cacttcatat ggcactttca tcgcggtcag ctgctgttcg
    36181 gttttgccca cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc
    36241 agatgcgcgg tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag
    36301 gccgctgatg ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt
    36361 gcggtctgat acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg
    36421 ttctgtaacg ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca
    36481 gctttcagtt ttttacccga cttcagatgc atgatcacac cgtcgtcaca gccttcgatc
    36541 ttctcgtact cttcgttgtg acgaatcact acgccactgt tccagaagtg ataggagaga
    36601 gaatctgaca tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc
    36661 acttttacat ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca
    36721 tagataagta catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg
    36781 cgtggatggg tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca
    36841 aatttttcag cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc
    36901 tcgtcaacaa agcgagcgtt tccctgcaat atttcacagt gattacgttc gtaaaatccc
    36961 tgacgcatgc gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa
    37021 gaagagcgga gcagtcggga atggtcgctg taaagtgggt tttgattgaa ttctataatg
    37081 cggctgacgg cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg
    37141 ccaacatttt gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt
    37201 gcagcgcctt cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt
    37261 ggcatggtag ggcttacctg ttcttataca taaaagcaac agaatggtaa cattttatcg
    37321 cgggtaagcc aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg
    37381 tctgtttctt acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt
    37441 aagcggcagg tttccgctgt acgtaaaaga accggccaaa gaattgcagt aaatatgttt
    37501 tattgcgtta ccgttcattc acaatactgg agcaatccag tatgttcatt ctctggtata
    37561 gtgccagcag tacttttggc aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa
    37621 aagaaaaaac ccgccgttcg ctggtggaag ccgcatttag ccaattaagt gctgaacgca
    37681 gcttcgccag cctgagtttg cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt
    37741 tttatcggca tttccgcgac gtagacgaac tgggtctgac catggttgat gagagcggtt
    37801 taatgctacg ccaactcatg cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga
    37861 tccgcacctc ggtctccaca tttatggagt tcatcggtaa taatcctaac gccttccggt
    37921 tattattgcg ggaacgctcc ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa
    37981 ttcagcactt cattgcggaa cttgcggact atctggaact cgaaaaccat atgccgcgtg
    38041 cgtttactga agcgcaagcc gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg
    38101 cgttggacgt cggcgtcgaa caacgtcggc aattagaaga gcgactggta ctgcaactgc
    38161 gaatgatttc gaaaggggct tattactggt atcgccgtga acaagagaaa accgcaatta
    38221 ttccgggaaa tgtgaaggac gagtaatgaa acaagcaaat caagatagag gtacgctgct
    38281 gctggcgttg gttgctggct tatcgattaa tggtactttc gcagcgctgt ttagctccat
    38341 tgtgccattt tctgtattcc cgattatttc cctggtgctg acggtttact gcctgcatca
    38401 acgttatctt aatcgcacca tgccggtagg cttgccgggt ctggcagctg cctgttttat
    38461 tctcggcgta ctgctgtaca gcacggtagt tcgtgcggaa tatccggata tcggctctaa
    38521 cttcttcccg gcagtactct ccgtcattat ggtgttctgg attggcgcga agatgcgtaa
    38581 ccgtaagcag gaagttgctg agtaatcggt attatgccgg atgctggcgc atccggcatg
    38641 ggttttactt cgcggtcagt aatacgccgc actccatatg gtgcgtgtag gggaactgat
    38701 caaacagagc cagacgttcg accttgtgcg tctggcttaa tgtttccaga ttcttgcata
    38761 acgtttccgg attgcaggag atatacagaa tacgtggata cgcctgcacc attttctcgg
    38821 tttcactgtc cagaccgctg cgcggtgggt cgacaaaaat ggtttcgcac tgataactct
    38881 tcaagtctat cccttgcagg cgattaaact cgcgtacacc attcatcgcc tgagtaaatt
    38941 cttctgccgc catacgaata atttgtacgt tatcaatatg gttagctgcg atgttgtatt
    39001 gcgcagcagc aaccgacggc ttagcgattt cggtggctaa tacccgatca aaattgcgcg
    39061 ccagtgctaa tgaaaagtta ccgttgccgc agtacagctc cagtaaatcg ccttttgagc
    39121 ctttggttac gtccagcgcc cattccagca tctgaatatt catcgccgcg ttcggctggg
    39181 taaaactgtt ttctacctga cggtagatca tctctttccc tgcgaccggc agacgttcat
    39241 cgatgtaatc ttgatccagc tcgattttgg ttttcgttgc ccgaccaatc agatgcacat
    39301 tcagattctg cgcgcgcagt gcatcgcgca gggcttccgc ttcctgacgc cactcatcat
    39361 ccagcttttt atggtacaac agggaaacca ccgcctgatt actcagcgtg gtgaggtaat
    39421 caatctggaa caacttgtgg cgcagaacgg gattattacg cacacccgca atcatcgccg
    39481 tcatcaactg gttgatgagt tcactggcag cggggaagct atccacgcgg atgcggcttt
    39541 tggtttgttg atcgaaaatg atgtgataca ggtcatcgcc atcgtgccag atgcggaact
    39601 ccgcacgcat ccggtaatga ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa
    39661 acggtgccat catactttgc aaacgtacca ctttttcggc taactgcgct tcatactgtt
    39721 ctgttggaag gtgttcgggg gtcatgatgt atcctgaaaa attaaagtac gcggcgattg
    39781 tagggattgc tcatcagatg tccagatctt gatgaattcc tatttgtgag ctacgtctgg
    39841 acagtaactt gttacaacct gtagcatcca cttgccggtc ctgtgagtta atagggaatc
    39901 cagtgcgaat ctggagctga cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg
    39961 gacactgcca tccggtggga agtcatcatc tcttagtatc ttagataccc ctccaagccc
    40021 gaagacctgc cggccaacgt cgcatctggt tctcatcatc gcgtaatatt gatgaaacct
    40081 gcggcatcct tcttctattg tggatgcttt acaatgatta aaaaagcttc gctgctgacg
    40141 gcgtgttctg tcacagcctt ttccgcttgg gcacaggata ccagcccgga tactctcgtc
    40201 gttactgcta accgttttga acagccgcgc agcactgtgc ttgcaccaac caccgttgtg
    40261 acccgtcagg atatcgaccg ctggcagtcg acctcggtca atgatgtgct gcgccgtctt
    40321 ccgggcgtcg atatcaccca aaacggcggt tcaggtcagc tctcatctat ttttattcgc
    40381 ggtacaaatg ccagtcatgt gttggtgtta attgatggcg tacgcctgaa tctggcgggg
    40441 gtgagtggtt ctgccgacct tagccagttc cctattgcgc ttgtccagcg tgttgaatat
    40501 atccgtgggc cgcgctccgc tgtttatggt tccgatgcaa taggcggggt ggtgaatatc
    40561 atcacgacgc gcgatgaacc cggaacggaa atttcagcag ggtggggaag caatagttat
    40621 cagaactatg atgtctctac gcagcaacaa ctgggggata agacacgggt aacgctgttg
    40681 ggcgattatg cccatactca tggttatgat gttgttgcct atggtaatac cggaacgcaa
    40741 gcgcagacag ataacgatgg ttttttaagt aaaacgcttt atggcgcgct ggagcataac
    40801 tttactgatg cctggagcgg ctttgtgcgc ggctatggct atgataaccg taccaattat
    40861 gacgcgtatt attctcccgg ttcaccgttg ctcgataccc gtaaactcta tagccaaagt
    40921 tgggacgccg ggctgcgcta taacggcgaa ctgattaaat cacaactcat taccagctat
    40981 agccatagca aagattacaa ctacgatccc cattttggtc gttatgattc gtcggcgacg
    41041 ctcgatgaga tgaagcaata caccgtccag tgggcaaaca atgtcatcgt tggtcacggt
    41101 agtattggtg cgggtgtcga ctggcagaaa cagactacga cgccgggtac aggttatgtt
    41161 gagaatggat atgatcaacg taataccggc atctatctga ccgggctgca acaagtcggc
    41221 gattttacct ttgaaggcgc agcacgcagt gacgataact cacagtttgg tcgtcatgga
    41281 acctggcaaa ccagcgccgg ttgggaattc atcgaaggtt atcgcttcat tgcttcctac
    41341 gggacatctt ataaggcacc aaatctgggg caactgtatg gcttctacgg aaatccgaat
    41401 ctggacccgg agaaaagcaa acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg
    41461 aactggcgta tttccggata tcgtaacgat gtcagtgact tgatcgatta tgatgatcac
    41521 accctgaaat attacaacga agggaaagcg cggattaagg gcgtcgaggc gaccgccaat
    41581 tttgataccg gaccactgac gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg
    41641 attaccgaca cgccgttgtt acgccgtgct aaacagcagg tgaaatacca gctcgactgg
    41701 cagttgtatg acttcgactg gggtattact tatcagtatt taggcactcg ctatgataag
    41761 gattactcat cttatcctta tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt
    41821 gcggttgcgt atccggtcac ctctcacctg acagttcgtg gtaaaatagc caacctgttc
    41881 gacaaagatt atgagacagt ctatggctac caaactgcag gacgggaata caccttgtct
    41941 ggcagctaca ccttctgaac cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg
    42001 gttgtcggtc tatgacgaga tccggcatct cttaccggat ctccattaca tttatgcttt
    42061 cgataacgtc gcttttccgt atggcgaaaa aagcgaagag tttattgttg agcgagtggt
    42121 ggcaattgtc accgcagtgc aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa
    42181 caccgccagt accgtttcac ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg
    42241 tgtcgtgccg gcgattaaac ctgctgcacg tctgacggca aatggcattg tcggattact
    42301 ggcaacccgc ggaactgtta aacgttctta tactcatgag ctgatcgcgc gtttcgctaa
    42361 tgaatgccag atagaaatgc tgggctcggc agagatggtt gagttggctg aagcgaagct
    42421 acatggcgaa gatgtttctc tggatgcact aaaacgtatc ctacgcccgt ggttaagaat
    42481 gaaagagccg ccagataccg ttgtattggg ttgcacccat ttccctctac tacaagaaga
    42541 actcttacag gtgctgccag agggaacccg gctggtggat tctggcgcag cgattgctcg
    42601 cagaacggcc tggctgttag aacatgaagc tccggatgca aaatctgccg atgcgaatat
    42661 tgccttttgt atggcaatga cgccagaagc tgaacaatta ttgcccgttt tacagcgtta
    42721 cggcttcgaa acgctcgaaa aactggcagt tttaggctga tttggttgaa tgttgcgcgg
    42781 tcagaaaatt attttaaatt tcctcttgtc aggccggaat aactccctat aatgcgccac
    42841 cactgacacg gaacaacggc aaacacgccg ccgggtcagc ggggttctcc tgagaactcc
    42901 gacagagaaa gcaaaaataa atgcttgact ctgtagcggg aaagcgtatt atgcacaccc
    42961 cgcgccgctg agaaaaagcg aagcg
//
LOCUS       DAZFXT010000026        42679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.026, whole genome shotgun sequence.
ACCESSION   DAZFXT010000026 DAZFXT010000000
VERSION     DAZFXT010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.026"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            34..399
                     /gene="yfiL"
                     /locus_tag="ACHKW0_001529"
     CDS             34..399
                     /gene="yfiL"
                     /locus_tag="ACHKW0_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HFO7474385.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            549..1067
                     /gene="yfiR"
                     /locus_tag="ACHKW0_001530"
     CDS             549..1067
                     /gene="yfiR"
                     /locus_tag="ACHKW0_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HFO7474386.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            1057..2283
                     /gene="dgcN"
                     /locus_tag="ACHKW0_001531"
     CDS             1057..2283
                     /gene="dgcN"
                     /locus_tag="ACHKW0_001531"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HFO7474387.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELAD
                     HNMYQAKHQRAEKLVR"
     gene            2299..2781
                     /gene="yfiB"
                     /locus_tag="ACHKW0_001532"
     CDS             2299..2781
                     /gene="yfiB"
                     /locus_tag="ACHKW0_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7474388.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(2858..3205)
                     /gene="rplS"
                     /locus_tag="ACHKW0_001533"
     CDS             complement(2858..3205)
                     /gene="rplS"
                     /locus_tag="ACHKW0_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HFO7474389.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(3247..4014)
                     /gene="trmD"
                     /locus_tag="ACHKW0_001534"
     CDS             complement(3247..4014)
                     /gene="trmD"
                     /locus_tag="ACHKW0_001534"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HFO7474390.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(4045..4593)
                     /gene="rimM"
                     /locus_tag="ACHKW0_001535"
     CDS             complement(4045..4593)
                     /gene="rimM"
                     /locus_tag="ACHKW0_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HFO7474391.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(4612..4860)
                     /gene="rpsP"
                     /locus_tag="ACHKW0_001536"
     CDS             complement(4612..4860)
                     /gene="rpsP"
                     /locus_tag="ACHKW0_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HFO7474392.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(4997..6358)
                     /gene="ffh"
                     /locus_tag="ACHKW0_001537"
     CDS             complement(4997..6358)
                     /gene="ffh"
                     /locus_tag="ACHKW0_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HFO7474393.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            6525..7316
                     /gene="ypjD"
                     /locus_tag="ACHKW0_001538"
     CDS             6525..7316
                     /gene="ypjD"
                     /locus_tag="ACHKW0_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HFO7474394.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            7337..8623
                     /gene="yfjD"
                     /locus_tag="ACHKW0_001539"
     CDS             7337..8623
                     /gene="yfjD"
                     /locus_tag="ACHKW0_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HFO7474395.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            8746..9351
                     /locus_tag="ACHKW0_001540"
     CDS             8746..9351
                     /locus_tag="ACHKW0_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoplasmic protein"
                     /protein_id="HFO7474396.1"
                     /translation="MSEDYVIEWDKDFADDLNVVASVFLQHTPTLWPTIFSQLSTQPE
                     IFEDEDEDEYGLQDVLDCSGGDLGNRDLAQAFLQVLRGEGLIQLVDWKGEDEEGELAN
                     FAADRFYELTKNLIASEELRSLLVEITQEDEISDVCEAGDRYLDEIFERIQTELNKRG
                     FQIFDLNEGSDTYNVVVLPMNEYKKIDDFNTPWLEVQDFLS"
     gene            complement(9386..9976)
                     /gene="grpE"
                     /locus_tag="ACHKW0_001541"
     CDS             complement(9386..9976)
                     /gene="grpE"
                     /locus_tag="ACHKW0_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HFO7474397.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAK"
     gene            10099..10977
                     /gene="nadK"
                     /locus_tag="ACHKW0_001542"
     CDS             10099..10977
                     /gene="nadK"
                     /locus_tag="ACHKW0_001542"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HFO7474398.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            11063..12724
                     /gene="recN"
                     /locus_tag="ACHKW0_001543"
     CDS             11063..12724
                     /gene="recN"
                     /locus_tag="ACHKW0_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HFO7474399.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            12873..13214
                     /gene="bamE"
                     /locus_tag="ACHKW0_001544"
     CDS             12873..13214
                     /gene="bamE"
                     /locus_tag="ACHKW0_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HFO7474400.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(13276..13566)
                     /gene="yfjF"
                     /locus_tag="ACHKW0_001545"
     CDS             complement(13276..13566)
                     /gene="yfjF"
                     /locus_tag="ACHKW0_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HFO7474401.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(13556..13993)
                     /gene="ratA"
                     /locus_tag="ACHKW0_001546"
     CDS             complement(13556..13993)
                     /gene="ratA"
                     /locus_tag="ACHKW0_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HFO7474402.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            14164..14646
                     /gene="smpB"
                     /locus_tag="ACHKW0_001547"
     CDS             14164..14646
                     /gene="smpB"
                     /locus_tag="ACHKW0_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HFO7474403.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            14861..15223
                     /gene="ssrA"
                     /locus_tag="ACHKW0_001548"
     tmRNA           14861..15223
                     /gene="ssrA"
                     /locus_tag="ACHKW0_001548"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            15458..16666
                     /locus_tag="ACHKW0_001549"
     CDS             15458..16666
                     /locus_tag="ACHKW0_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009036 - type II site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II restriction endonuclease"
                     /protein_id="HFO7474404.1"
                     /translation="MSVFHNWLLDIASGNYFIYIKRLSANDTGATGGHQVGLYIPSNI
                     VEKLFPSINHTRELNPSVFLTAHVSSHDCPDTQARAIYYNNRYFGKTRNEKRITRWGR
                     GSPLQNPENTGALTILAFRLNEQNTDCSEVDIWVCVNPDEEDIIESAIGEIIPGTLIS
                     GPAGQILGGLSLQQTPVNHKYVIPEDWKKRFPSGNEIIQYAAGHYAKNSKDPDEQLID
                     RRRVEYDIFLLVEELHVIDIIKKGFDSVDEFIALANSVSNRRKSRAGKSLELHLEKLF
                     IEHGLRHFSTQAVTEGNKKPDFLFPSAEAYHNVEFPVENLRMLAVKTTCKDRWRQILN
                     EADKIHQVHLFTLQEGVSSAQYREMKDAGVRLVVPSTLHKKYPEAVREELITLGAFIT
                     ELIELYAELS"
     gene            complement(16701..18134)
                     /locus_tag="ACHKW0_001550"
     CDS             complement(16701..18134)
                     /locus_tag="ACHKW0_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HFO7474405.1"
                     /translation="MSEFEVLAQHLLKEAEAEEKLRQENDKKLIEKVLEIYDQKYVAE
                     LLRKVGKNEWSRETINRWINGKCLPKSLTSVEESLLRKMLPEPPANHPEYAFRFIDLF
                     AGIGGIRKGFEAIGGQCVFTSEWNKDAVRTYKANWFNDEQVHKFNLDIREVTLSDKTD
                     VLETDAYAYIDEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDV
                     ARIIRAKKPAIFVLENVKNLKSHDKGKTFKVIMETLDELGYEVADAAEMGKNDPKIID
                     GKHFLPQHRERIVLVGFRRDLNIHKGFTLRDISRFYPEHRPSFGELLEPVVDSKYILT
                     PKLWEYLYNYAKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMA
                     TGEADFMNESNQARRPRRLTPRECARLMGFEKPGGKPFRIPVSDTQSYRQFGNSVVVP
                     VFEAVARLLEPYILKAVSADAGKTGQP"
     gene            18541..19314
                     /locus_tag="ACHKW0_001551"
     CDS             18541..19314
                     /locus_tag="ACHKW0_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474406.1"
                     /translation="MDITEFPSGVIEHLGWYVYRLIDPRDGSTFYVGKGKGNRVFAHM
                     RGEVAATDDDELLSNKLKQIREIRLAGLEVIHVIHRHGMTDEKTAYEVEAALIDAYPG
                     LTNIMNGAGSNEFGAAHVKELIATYQPETIIFHHKALMISVNRSAKDSELYDAVRFSW
                     RINVSRASQAEVILATVRGIVRGVFIADKWLKSTRENFPTMKYWDEDPDFEATQSSRY
                     GFEGREAPPEIANLYLGKKIPDELRKKGAMSPVRYSPNF"
     gene            complement(19372..19926)
                     /gene="pinE"
                     /locus_tag="ACHKW0_001552"
     CDS             complement(19372..19926)
                     /gene="pinE"
                     /locus_tag="ACHKW0_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
                     /protein_id="HFO7474407.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCELIFEDKISGTKSERPG
                     LKKLLRTLSEGDTLVVWKLDRLGRSMRHLVILVEELRERGVNFRSLTDAIDTSTPMGR
                     FFFHVMGALAEMERELIVERTKAGLEAARAQGRIGGRRPKLTPEQWAQAGRLIASGVP
                     RQKVAIIYDVGISTLYKKFSVGDK"
     gene            19953..20354
                     /locus_tag="ACHKW0_001553"
     CDS             19953..20354
                     /locus_tag="ACHKW0_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7474408.1"
                     /translation="MMPFARYFCIFINVGLGEAAKRDVGTGENQIPDMSAWKRNPSSN
                     RWRKLPDGTIIQMGISASGPLGSPVNITLPISFSNTNYCVVASYDNARSGVSTMVSFA
                     ALPVSPSQFSLMSSVTEQGINPFAYWIAFGD"
     gene            20358..20777
                     /locus_tag="ACHKW0_001554"
     CDS             20358..20777
                     /locus_tag="ACHKW0_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HFO7474409.1"
                     /translation="MDRYFYSQKENGFFTDLNKAPSDAVEITTDEWLSLLDGQDNGMK
                     IVSNQEGYPVLTEQPPLSKENLIALAELKKGKLINEANEHMNSRQWPGKAAIGRLKGE
                     ELAQYNLWLDYLDALELVDTSSAPDIEWPTPPAVQAR"
     gene            complement(20749..21342)
                     /locus_tag="ACHKW0_001555"
     CDS             complement(20749..21342)
                     /locus_tag="ACHKW0_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7474410.1"
                     /translation="MKPVFDENGLATVPGDMRCFYYDAETSEYTGWSDEYINTGVSMP
                     ACSTGIDPGENIPGKVAVFTGKGWSHEEDHRNETVYSTENGAAVTVDYIGAIKDGYVT
                     LSPLTPYDKWDGEKWVTDTEAQHSAAVDAAEAQRQSLIDTVMASISLIQLKLQAGRKL
                     TQAETTRLNAVLDYIDAVTVTDTSTAPDIIWPELPEA"
     gene            complement(21342..22088)
                     /locus_tag="ACHKW0_001556"
     CDS             complement(21342..22088)
                     /locus_tag="ACHKW0_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO7474411.1"
                     /translation="MHRIDTKTAQKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEG
                     SPAIGVPFFWPSAAMPNTVIDSWSSMVFLKFNGAKFSATDYPVLAKVFPSLVLPEARG
                     DFIRIWDDGRGADGGRELLSWQAATNFSQFAGNIGEGAGHAINFHDGIAGNQPGFSRF
                     NFTSNSVGDGVNFVAVRPRNIAFNFLVRAK"
     gene            complement(22092..22676)
                     /gene="ymfQ"
                     /locus_tag="ACHKW0_001557"
     CDS             complement(22092..22676)
                     /gene="ymfQ"
                     /locus_tag="ACHKW0_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfQ family protein"
                     /protein_id="HFO7474412.1"
                     /translation="MDVTNDDYIRLLSALLPPGPAWSASDPAIAGAAPSLTRVHQRAD
                     ALMRELDPRTTTELINRWERLCGLPDECIPAGTQTLRQRQQRLDAKVNLSGGINEDFY
                     LAQLAALGRPDATITRYDKSTFTCSSACTDAVNAPEWRYYWQVNMPAATNTTWMTCGD
                     PCDSALRIWGDTVVECVLNKLCPSHTYVIFKYPE"
     gene            complement(22667..23725)
                     /locus_tag="ACHKW0_001558"
     CDS             complement(22667..23725)
                     /locus_tag="ACHKW0_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7474413.1"
                     /translation="MADSEFQRPTLAENISMLRNDLFARLDVSDTLRRMDEDVRAKVY
                     AAALHTVYGYIDYLAMNMLPDLCDESWLARHAAMKRCPRKGATAASGYMRWEGVSDGL
                     KVTAGSVIQRDDLVQYTATADATSSGGVLRVPITCSTTGAVGNADDGTALILVTPVNG
                     LPSSGVADTLTGGFDTEDLETWRARVIERYYWTPQGGADGDYVVWAKEVPGITRAWTY
                     RHWMGTGTVGVMIASSDLINPIPEESTETAARQHIGPLAPVAGSDLYVFRPVAHKVDF
                     HIRVTPDTPEIRAAITAELRSFLLRDGYPQGELKVSRISEAISGANGEYSHQLLAPAD
                     NISIAKNELAVLGTISWT"
     gene            complement(23712..24137)
                     /locus_tag="ACHKW0_001559"
     CDS             complement(23712..24137)
                     /locus_tag="ACHKW0_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HFO7474414.1"
                     /translation="MELWLTVNGKRTCASAPLDPLTRAVVISLFTWRRAEPDDNADVP
                     MGWWGDTWPAVQNDRYGSRLWLLQRSKLTNQLVQTVRGYIRECLQWMTDDGVVSRIDL
                     DIRRTGINELGNSITLWRRDGPVMISFDDLWSAITHGGQ"
     gene            complement(24137..24685)
                     /locus_tag="ACHKW0_001560"
     CDS             complement(24137..24685)
                     /locus_tag="ACHKW0_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO7474415.1"
                     /translation="MRTIETMQRQLLGLIGRAVVKSISAATKCQTVDVSLIAGEPKAG
                     VEHLEPYGFTARANSGAEAVVLFPDGDRSHAVVVTVSDRRYRLKGLQTGEVAVYDDQG
                     QSVTLTREGIVVDGAGKTITFRNAPRARFEMDLEVTGQVKDLCDSGGTTMSAMRLAYN
                     GHRHRENGQGSNTDKPDKAMEA"
     gene            complement(24685..25764)
                     /locus_tag="ACHKW0_001561"
     CDS             complement(24685..25764)
                     /locus_tag="ACHKW0_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HFO7474416.1"
                     /translation="MNDNVTLRVNGREWNGWTSVRIGAGIERLARDFSVEITRQWPGD
                     EGITTLQPRIKNGSKVEVLIGDELVITGWVEATPVRYDARSVSTGIAGRSLTADLIDC
                     AAEPTQFNGRSLVQIAQALAAPFGIEVVNSGAPSGVIPDVQPDHGETVIEVINKILGQ
                     QQALAYDDPHGRLVIGGIGSTRAHTALVLGENILSCDTEKSIRERFSVYQVAGQRAGN
                     DDDFGEATTTALRARTEDAFIARYRPMYIRQTGQATGAGCIARADFEARQRAARTDET
                     TYVVQGWRQGNGTLWQPNQRVIVFDPVCGFDNTELLVSEVTFTQDQNGTQTEIRVGPP
                     DAYLPEPEAPGARKKKKARVQEDPF"
     gene            complement(25761..27089)
                     /locus_tag="ACHKW0_001562"
     CDS             complement(25761..27089)
                     /locus_tag="ACHKW0_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HFO7474417.1"
                     /translation="MTWKDRLQDASFRGVPFKVEEESAGTGRRVETHEYPNRDKPYTE
                     DLGKITFRPSITAYVVGDDCFDQRDRLIDALNKPGPGTLVHPTYGELKVCVDGEVRVS
                     TSKSEGRIVRFDLKFVEAGELSYPTSGAATAQTLMSSCSALDDCISDSFSGFSIDGVA
                     DFVQNDVVGNAGTMLGYVSDAMKVVDSAVSDAARLLQGDISVLLPPPSSGKNFVEQVQ
                     KMWRTGKRLYGNASDLVTMIKTLSGVSLGSDLQPRGVWKTDSKTTATATQQRNVVASI
                     LRTTAISEAAYAVTRLPAPTTSAVMQNAAVGQATTPAQSTGWPSVTHPALNNAPAVKN
                     TVDLPTWEELTDIRDTLNTAIDKELSRTTSDALFLALRRVKADLNADINTRLEQSARI
                     IQRTPDEVLPALVLAATWFDNAARDADIIRRNAITHPGFVPVIPLKVPVQ"
     gene            complement(27150..28985)
                     /locus_tag="ACHKW0_001563"
     CDS             complement(27150..28985)
                     /locus_tag="ACHKW0_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7474418.1"
                     /translation="MAEFELKALITGVDRLSPALSKMQKKIRGFKRQAEEASQGGLAL
                     GGGLAAGLTLSLKSYADQENAATGLKVAMMDANGEVGKRFQDINKLAIGLGNQLPGTT
                     ADFQNMMQMLVRQGIPAENILGGVGKATAYLAVQLKKTPEAAAEFAAKMQDATGTASE
                     DMMGLFDTIQKAFYLGVDDTNMLSFFTKTSSVLKMVNKDGLQAAQSLAPISVMMDQMG
                     MNGESAGNALRKVIQSGLSVKKIRDVNKIMARQKLGVQLDFTDGKGSFGGLDNMFRQL
                     AKLRKLTDVKRTGVLKAIFGDDAETLQVVNALIDKGKDGYDQIQQKMNKQASLNKRVQ
                     AQLGTLSNLWEAMTGTATNGLAAIGGAFSGDAKNITQWLGELGEKFTKFADENPRVIR
                     GVVGLAAGLAILKLGLMGVGSAISIVSRLMSMTPIGMIATAIALAAGLIITNWDVVGP
                     YFKKLWETIGPYFETGWELLKKVFAWSPLGMVINNWGPVVKWFQDMWDKLKPIIEWFT
                     DSSGDTVDAINSAQWGAGAYDAYGTGIPARGYTPYPAVDPAQSNNASDATGPNPFMIN
                     KASAPKVDGEIKVSFVNSPPGMRVMETRSSGFDVSHDVGYTRFGR"
     gene            complement(29127..29396)
                     /locus_tag="ACHKW0_001564"
     CDS             complement(29127..29396)
                     /locus_tag="ACHKW0_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001526907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098003 - viral tail assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HFO7474419.1"
                     /translation="MKELELKKPITAHGETLSVLEFDEPTGKDVRELGYPYQMNQDES
                     VRLLAHVVSKYIVRLAKVPQSSVDQMSPADLNAAAWLVAGFFLQA"
     gene            complement(29396..29752)
                     /locus_tag="ACHKW0_001565"
     CDS             complement(29396..29752)
                     /locus_tag="ACHKW0_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HFO7474420.1"
                     /translation="MARIGGTCYFKIDGQQLSLTGGIEVPMNRMVNDDIIGLDGSVDR
                     KETHRAPYVKGTFKVPKNFPVGKITSSDEMTITAELANGQVYVLSSAWLHGEANHNAE
                     EGTVDLEFHGEEGDYQ"
     gene            complement(29752..31248)
                     /locus_tag="ACHKW0_001566"
     CDS             complement(29752..31248)
                     /locus_tag="ACHKW0_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO7474421.1"
                     /translation="MTISFNTIPSNTLVPIFYAEMDNQAANTAQDSGASLLIGHANNG
                     AEIVANSLVLMPSADYARQICGAGSQLARMVEAYRQTDPFGELYVIAVPESTGAAATV
                     TLTVTGAATETGTVNVYVGRTRVQAPVTNGDNVTTIASSIKDAINAVPALPFTASSSA
                     GVVTLTARHKGLCGNEIPVSLNYYGFGGGEVLPAGVQIAVATGTAGTGAPVLTGAVAA
                     MADEPFDYIGLPFNDTASVNTLVTEMNDTSGRWSYARQLYGHVYTAKIGTLSELVTAG
                     DQFNQQHITLAGYEKDTQTPADELAASRTARAAVFIRNDPARPTQTGELVGMLSAPKG
                     KRFTMTEQQTLLSHGVATAYVESGVLRIQRDVTTYRKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLASDGTRFGPGQAIVTPAVIKGELLATYRQLERAGIVENYE
                     LFKQYLVVERDASDPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
     gene            complement(31232..31402)
                     /locus_tag="ACHKW0_001567"
     CDS             complement(31232..31402)
                     /locus_tag="ACHKW0_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2635 domain-containing protein"
                     /protein_id="HFO7474422.1"
                     /translation="MFVKPVKGRSVPDPARGDLLPAEGRNVDENNYWLRREAAGDIRR
                     VNEKVNTDDDKL"
     gene            complement(31411..31971)
                     /locus_tag="ACHKW0_001568"
     CDS             complement(31411..31971)
                     /locus_tag="ACHKW0_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474423.1"
                     /translation="MKLTPVIAALRARCPYFENRVAGAAQFKNLPEVGKLKLPAAYVV
                     PGDDSPGENKSQTDYWQELKEGFSVVVILSNGRDERGQFASYDVVDDVRQMLFKALLG
                     WNPEACGNPITYDGGTLLDLNRHELIYQFDFSVISELTEDDTRQQDDLNSLDELRTLA
                     IDVDYLDPGNGPDGDIEHHTEITLPS"
     gene            complement(31968..32474)
                     /locus_tag="ACHKW0_001569"
     CDS             complement(31968..32474)
                     /locus_tag="ACHKW0_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474424.1"
                     /translation="MTTSFLHVDFQQPAEMRFNRARVRRAFVTIGQRHMRDARRLVMR
                     RARSAPGENPGYQTGRLARSIGYMVPRASKHRPGFMARIAPNQRNGEGNRRITGDFYP
                     AFLFYGVRRGAKRRRGHHRGASGGSGWRLAPRNNFMVETLEKNRSWTRYFLARELRKS
                     LKPERRHR"
     gene            complement(32449..32859)
                     /locus_tag="ACHKW0_001570"
     CDS             complement(32449..32859)
                     /locus_tag="ACHKW0_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7474425.1"
                     /translation="MKIRQAQTSATYILPDPGELNKRVLIRQRVDMPADNFGVEPQYP
                     VTFRTWAKVIQTSATTWQETAQTGDAITHYITIRYRRGITADYEVVCGDSVYRVKRQR
                     DLNGARRFLLLECTELGECRQSHGGNNDDFLFAR"
     gene            complement(32856..33179)
                     /locus_tag="ACHKW0_001571"
     CDS             complement(32856..33179)
                     /locus_tag="ACHKW0_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001492759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO7474426.1"
                     /translation="MLLTMEEIKAQLRLDEDFDADDRHLQLLACAAQKRTETYLNRKL
                     YAPDETIPDSDPDGLHLPDDIRLGMLMLISHFYENRSSVTDVEKMELPMSFNWLVGPY
                     RYFPQ"
     gene            complement(33182..33382)
                     /locus_tag="ACHKW0_001572"
     CDS             complement(33182..33382)
                     /locus_tag="ACHKW0_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001735262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474427.1"
                     /translation="MILKQDLKWSPDGMRVEVIRAGEYDDGALPARVQEIALQAGLAE
                     RGTSAKSSKATKEKKATTSKEG"
     gene            complement(33432..34637)
                     /locus_tag="ACHKW0_001573"
     CDS             complement(33432..34637)
                     /locus_tag="ACHKW0_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7474428.1"
                     /translation="MAVDIKDVEQVAQELQQKFDDFKAKNDKRVDAIEQEKGKLAGQV
                     ETLNGKLSELENLKSDLEKELLELKRPAGGAQNKLATEHKEAFVGFLRKGREDGLRDL
                     ERKALQVGTDEDGGYAVPEELDRNILNLLKDEVVMRQEATVITVGGSDYKKLVNLGGT
                     ASGWVGETDTRSQTATSRLELIEPLMGEIYGNPQATQKMLDDAFFNVEAWINSELATE
                     FAEQEEIAFTSGDGTKKPKGFLAYESTDETDKVRAFGKLQHIVSGEATAVTADAIIKL
                     IYTLRKAHRTGAKFMMNNNSLFAIRLLKDTEGNYLWRPGLELGQPSSLAGYGIAENEQ
                     MPDIAADAKAIAFGNFKRGYTIVDRIGTRILRDPYTNKPFVGFYTTKRTGGMLVDSQA
                     IKLLKIAAA"
     gene            complement(34652..35302)
                     /locus_tag="ACHKW0_001574"
     CDS             complement(34652..35302)
                     /locus_tag="ACHKW0_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO7474429.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKDWEYDRSKEAFLLKEIDLWEVSLVTFPSNDEARISDVKNAL
                     ARGEIPEQKKIERVLRDVGLSRTQAKAFMAGGYGALSLRDAEDVGSALNALKNLNF"
     gene            complement(35280..36521)
                     /locus_tag="ACHKW0_001575"
     CDS             complement(35280..36521)
                     /locus_tag="ACHKW0_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7474430.1"
                     /translation="MFFSGLFQRKSDAPVTTPAELADAIGLSYDTYTGKQISSQRAMR
                     LTAVFSCVRVLAESVGMLPCNLYHLNGSLKQRATGERLHKLISTHPNGYMTPQEFWEL
                     VVTCLCLRGNFYAYKVKAFGEVAELLPVDPGCVEPKLNSSWEPIYQVTFPDGSTDVLS
                     QEDIWHVRTLTLDGLVGLNPIAYAREAISLAAATEEHGARLFSNGAVTSGVLRTEQTL
                     SDQAYERLKKDFEERHTGLGNAHRPMILEMGLDWKSMALNAEDSQFLETRKFQLEEIC
                     RLFRVPLHMVQNTDRATFNNIEELGLGFINYSLVPYLTRIEQRINTGLVRKSKQGVYY
                     AKFNAGALLRGDMKSRFEAYATGINWGIYSPNDCRDLEDMNPRPGGDVYLTPMNMTTK
                     PSDGSKAGKQKDNANADETTS"
     gene            complement(36521..36703)
                     /gene="ymfR"
                     /locus_tag="ACHKW0_001576"
     CDS             complement(36521..36703)
                     /gene="ymfR"
                     /locus_tag="ACHKW0_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfR"
                     /protein_id="HFO7474431.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTQLSVGGGK"
     gene            complement(36715..38448)
                     /locus_tag="ACHKW0_001577"
     CDS             complement(36715..38448)
                     /locus_tag="ACHKW0_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7474432.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVVCCAFGWVNKG
                     SRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNINRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTDPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIIQNYTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKTIPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(38445..38939)
                     /locus_tag="ACHKW0_001578"
     CDS             complement(38445..38939)
                     /locus_tag="ACHKW0_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO7474433.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEENLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(39065..39415)
                     /locus_tag="ACHKW0_001579"
     CDS             complement(39065..39415)
                     /locus_tag="ACHKW0_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO7474434.1"
                     /translation="MPPRIPKACRARGCRNTTTDPSGYCESHKSEGWKQYKPGQSRHQ
                     RGYGSKWDSIRARVLKRDKGLCQLCLRSGVVREAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERLK"
     gene            complement(39628..39879)
                     /locus_tag="ACHKW0_001580"
     CDS             complement(39628..39879)
                     /locus_tag="ACHKW0_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474435.1"
                     /translation="MSSTKLVKTILFHGDNKYEVYATSEYADVDRIIHYKLRSCKNTD
                     GSDGKSESIWVVYNPAVQLPDDLNYKKVSNIAPYLEKLQ"
     gene            complement(40309..40746)
                     /locus_tag="ACHKW0_001581"
     CDS             complement(40309..40746)
                     /locus_tag="ACHKW0_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7474436.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAMTYKEQRDKAASII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVAATCAKSTTGASSM
                     GDGESPRLTADAELNYYRLRSGINRITAQVNYLQEYIRTQCLK"
     gene            complement(40743..41219)
                     /locus_tag="ACHKW0_001582"
     CDS             complement(40743..41219)
                     /locus_tag="ACHKW0_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7474437.1"
                     /translation="MQVLNSQRKAFLDMVAWSEGTDNGRQPTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPRSQDSVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQFEHKADSLIAKFKEAGGTVREIEV"
     gene            complement(41223..41549)
                     /locus_tag="ACHKW0_001583"
     CDS             complement(41223..41549)
                     /locus_tag="ACHKW0_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HFO7474438.1"
                     /translation="MPHNPNTWLELVQSWWRGDTPLGAVIMSIVMAGLRIAYFGGGGG
                     WKRKTLEILLCGALTLTFASALEYVGWPKSLSVAIGGGVGLIGVDAIRGAAMRVIGNK
                     FGGSKE"
     gene            41841..>42679
                     /locus_tag="ACHKW0_001584"
     CDS             41841..>42679
                     /locus_tag="ACHKW0_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009295 - nucleoid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HFO7474439.1"
                     /translation="MDITCLECGNVLDDPTVACDKCGATPHVVVLGKQSYFPIGAVTA
                     NLEKNDSRAFDYRLGEVWDLKNEVTSEFITRIEKKFSRKNKFHNFLDSDHNPSSIPTI
                     LKKYINKNNEFIDLSRAIIEKLKHNANNESRVAQLQGGSVVFIHYKSTEPEDLGKLLI
                     VMVDKQSAYDFDSDKLTPTRLNPINTDALRQAAMFDLTLFEASYPENKGDSYVHFLQG
                     KSKSDFFKDSLGCRHDSDNKRSIQQLFSAIDIFASKNSLGRVLRDTIDNEVKSLLEKK
                     SKD"
BASE COUNT        10470 a        11578 c        10107 g        10524 t
ORIGIN      
        1 acaatccgct atgctttgaa aaaggagaaa gaaatgatga aaaagtttat cgcccccttg
       61 ttggctttac tggttagcgg atgtcagatt gatccttata ctcacgcgcc aaccttgacc
      121 agcaccgact ggtatgatgt tggtatggaa gatgcgatat cgggcagcgc cataaaagat
      181 gacgatgcat ttagcgattc acaggcggat cgcggtctat accttaaagg atatgccgaa
      241 ggacaaaaga aaacttgcca gaccgatttt acttatgccc ggggactttc cggtaaaagc
      301 tttcctgcca gctgcaacaa tgttgaaaat gccagccaac tgcatgaagt atggcaaaaa
      361 ggggctgatg aaaatgccag cacaatacga ttaaattaat taaaacccca caaataaatt
      421 aaacataaga ttttactcat tgtgttttat ttctcacatt gatgacgatc gcatgaaaaa
      481 atgatatttt aatgagcatt ctgaaaaaat atcgatgcat ttcgagcgaa gatggtgggg
      541 atccctgaat gcgtttttct caccgactgt tcttacttct gattctcctg ctgacgggag
      601 caccaatact cgctcaggag ccttctgacg ttgcgaaaaa cgtgcgtatg atggtttccg
      661 gtattgtcag ttacacccgc tggcctgcgc tatcagggcc accaaagtta tgtatctttt
      721 catcttcacg ttttagtacc gccctacaag agaatgccgc gacatctttg ccttatctgc
      781 ctgtcatcat tcacactcaa caagaggcga tgatttcagg ctgcaatggt ttttattttg
      841 gcaacgagtc accgacattc cagatggaat taaccgaaca atatccgtca aaggcgttat
      901 tattaattgc cgaacaaaat accgagtgca ttattggtag cgccttttgt ctgatcatcc
      961 acaataatga cgtcagattt gccgtaaacc tggatgcctt atcgcgtagc ggtgtaaagg
     1021 tcaacccgga tgtcttaatg ctcgcacgga agaaaaatga tggataacga taattctctt
     1081 aataagcgcc ccacgtttaa aagagcatta cgcaacatca gtatgaccag catatttatc
     1141 actatgatgc tgatctggtt gctgctttcc gtgacctcgg tgctgaccct gaaacagtac
     1201 gcgcaaaaaa acctggcact gacagcagca acaatgactt acagtctgga agcagctgtc
     1261 gtttttgccg atggccctgc agcaactgaa acactggcag cgctgggcca gcaagggcaa
     1321 ttttcaactg cagaagtacg tgataagcag caaaatattc tggcgtcctg gcattacacc
     1381 cgtaaggatc caggcgatac tttcagcaat ttcataagcc actggctctt ccccgccccc
     1441 atcattcagc cgattcgtca caatggtgaa accattggcg aagtacgctt aaccgctcgc
     1501 gacagttcaa tcagccattt tatctggttt tcgctcgccg tactgaccgg ttgtattctg
     1561 ctggcatcag gcatcgcaat taccctcacc cgccatttgc acaatggcct ggtagaagcg
     1621 ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca accgcaattt ttcccgacga
     1681 gtttcggaag aacgtatcgc tgagtttcac cgcttcgctc tcgacttcaa cagtctgctg
     1741 gatgaaatgg aagagtggca gcttcgttta caggccaaaa atgcgcagct tctgcgtacc
     1801 gcgctacatg acccattaac cgggctggct aaccgcgcag cgtttcgtag cggcatcaat
     1861 acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct tgatggcgat
     1921 aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagagt cttgattgaa
     1981 atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg cctgggcggc
     2041 gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca gcagatatgc
     2101 tcagcactga cacaaatctt taatctccct tttgatcttc ataatggcca tcagaccacc
     2161 atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga aaaattacaa
     2221 gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa gctggtgaga
     2281 taacaaggat atatcgatat gataaagcac ctggtagcac ccctgatttt cacctcacta
     2341 atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt cgccgctatg
     2401 caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc agatgccatt
     2461 ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat ccaaaccatg
     2521 gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca caccgataac
     2581 tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt cgtggccgat
     2641 gcatgggcta tgggtggaca aattccacgc agcaatctca ccacacaggg tttaggaaaa
     2701 aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg ccgcgtcgca
     2761 gtggtgatta caacccctta aaacacgagc aaacagacaa aaaaagggcc agccaattgg
     2821 ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc tctttgatac
     2881 gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga acagcaccac
     2941 gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca cgctcaacgc
     3001 cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg ttacgaatag
     3061 cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca acccatactt
     3121 tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc tgctcttgtt
     3181 caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa ctgatatctc
     3241 gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg ttttgaactc
     3301 cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt caggtcttct
     3361 aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg catggttgcc
     3421 cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg tatagtgcgg
     3481 gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat gtcccagtac
     3541 tcccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact caccaccact
     3601 gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca cgcgctcatc
     3661 tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca gttcgctgac
     3721 gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg cgccttcacc
     3781 cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca ttaacatccc
     3841 cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg tgaagtcgcg
     3901 aggactccaa ctctggatgc tcagcaggcc atttttaact gcccggccag ttaccccgta
     3961 atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc acatagcgcc
     4021 gtcttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt cgattgaacg
     4081 agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga gacgttcctt
     4141 gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag atccggtttc
     4201 catcatatcg acgactttac cgagatcgta gccttcagtg gttactacct ggcagcccat
     4261 caggtctttc cagtagtagt caccctcttc aagctgaggc agctgcgatg aatccacgac
     4321 aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt tcagcttgat
     4381 gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc actgacccgc
     4441 cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg tggaagaaaa
     4501 cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa cgatgggatc
     4561 aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag attaagctgc
     4621 tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc cctggccaac
     4681 ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc tagcgattgg
     4741 gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc tgtcagcgac
     4801 aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa tagttaccat
     4861 aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt ccggtgtcat
     4921 attaaaagcc cgaaaatttt actgatttct ggagaaaatg caatcagcag ttaataactc
     4981 tgtcgcataa ggtcatttag cgaccaggaa atcccggcgg catcatcccc ttcatgcttc
     5041 tcatcatctt cgccattccg cctttcttca ttttcttcat catgcgctgc atgtcgtcga
     5101 actgcttcag aaggcggttc acgtcctgca cctgcatacc gcaaccggca gcgatacgac
     5161 gcttacgcga acctttgatg atttctggct tagcgcgctc tttcatcgtc atcgagttga
     5221 tgatggcttc catacgcacc agcactttat cgtccatctg cgacttaaca ttatccggga
     5281 tctgccccat gcccggcagc ttgcccatca ggctagccat gccgcccata tttttcatct
     5341 ggcgcagttg ctcaagaaaa tcgttgagat cgaagccgtc gccttttttc agcttgctgg
     5401 ctaatttctc tgcctgcgcg cggtcaactt tgctttcgat atcttcgatc agcgacagta
     5461 cgtcgcccat gccgagaata cgcgacgcga tgcggtccgg atggaacggc tccagcgcct
     5521 cagttttctc gccaacaccg agaaacttga tcggtttgcc agtgatgtga cgaatagaga
     5581 gcgccgcacc gccgcgggca tcgccgtcca ctttggtcaa cactacgccg gtaagcggta
     5641 acgcttcatt gaatgctttt gccgtattgg ccgcatcctg accggtcatg gcgtcaacca
     5701 caaacagggt ttcaaccggg ttaatcgacg catggacttg tttgatctcg tccatcatcg
     5761 cttcgtcaac gtgcagacga ccagcggtat ccaccagcag cacgtcgtag aatttcagtt
     5821 tggcttcttt cagcgccgcg ttaacgatat ctaccggctt ctgaccaaca tcagaaggga
     5881 agaaatcaac gcccacctgc tctgccagcg tctcaagctg tttgattgcc gccgggcgat
     5941 aaacgtcggc agaaaccacc agcactttct tcttgtgctt ctcgcgcagg aacttaccga
     6001 gtttaccaac gctggttgtt ttaccggcac cttgcaggcc cgccatcagt acgaccgcag
     6061 gcggttgcgc agccaggttc agggtctggt tctcttcgcc catcgccgca accagttcgt
     6121 tacggactat tttgacgaac tcctgccccg gcgtcaggct cttattaact tcatgaccaa
     6181 ccgctttctc ttttacgcga ttgataaact cacgcactac cggcagagcg acgtccgcct
     6241 ccagcagcgc catgcgcact tcgcgcagcg tatcttttac gttgtcttca gtgaggcgtc
     6301 cacggccact gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta tcaaacattg
     6361 tctctcgcct ggggtggaaa cggttggccg caatcgcgac acatcatcag tattttgccg
     6421 cagtataaca tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt tcacctgacg
     6481 ctatactgct tctctttctt attgctcaaa ctgtcgacat tactatgccc gtttttgctc
     6541 tgctcgcgct tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt ctgctgcaaa
     6601 aaaacggcgg ctggcggcgc atggctatta tttctgcggt cattgcgctg gtctgccacg
     6661 caatcgctct ggaagcccgc atcctgcccg acggtgatag cggacaaaac ctcagcctgc
     6721 tgaacgttgg ttcattggtc agtttgatga tctgtacggt aatgaccatt gtggcttctc
     6781 gcaatcgtgg ctggttgctg ctacccattg tctatgcctt tgcgcttatc aacctggcgc
     6841 tggcgacctt catgcccaat gaatacatca cccatctgga agcaacgcct gggatgctgg
     6901 tgcacattgg cttatcgctc ttttcctatg ccacgctaat tatcgccgcc ctgtacgcgc
     6961 tgcaactggc gtggattgat taccaactga agaacaagaa gctggcattt aaccaggaaa
     7021 tgccgccatt gatgagtatc gagcgtaaaa tgttccacat cacgcagatt ggcgtggtgc
     7081 tgctaacgct cacgctttgc actggcctgt tctacatgca caacctattt agcatggaaa
     7141 atatcgacaa ggctgtgctc tctatcgtgg cgtggtttgt ctatattgtg ctgctgtggg
     7201 gacattacca tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt gcgggcgcgg
     7261 taattctgac actggcctac ttcggcagcc gaattgtcca gcagttaatc agctaaaccc
     7321 agaaaaggag tttcccctgg aacacatttc tactactacg ttgatcatta ttctgatcat
     7381 catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga ccctcaaccg
     7441 ctatcgtctg cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg tcgaaaaatt
     7501 gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc tggtcaatat
     7561 tcttgcctcc gcgctcggca ctattgttgg gatgcgtttg tacggcgatg cgggcgtggc
     7621 aattgcgact ggtgtgctga cttttgtcgt actggtattt gctgaggtat tgccgaaaac
     7681 cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc tggctccgct
     7741 gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta tgctgatgcg
     7801 catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag aagagttgcg
     7861 cactatcgtg cacgaatcac gctcacaaat ttcccgtcgc aatcaggata tgctgctgtc
     7921 ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtgccgcgca gtgaaattat
     7981 cggtattgat atcaacgatg actggaaatc gattctgcgc caactctccc actcacctca
     8041 cgggcgcatc gtgctctacc gtgattcgct ggacgacgcc atcagtatgc tgcgagtacg
     8101 tgaagcctgg cggttgatgt cggagaaaaa agagttcacc aaagaaacca tgctgcgcgc
     8161 cgcggacgag atctattttg tgccggaagg tacgccgctc agcacgcagt tggtaaagtt
     8221 tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca ttcaggggct
     8281 ggtgacggtt gaagatattc tggaagagat tgtcggcgat ttcactacgt cgatgtcgcc
     8341 aacacttgcc gaagaggtca cgccgcaaaa cgacggttcg gtgattatcg atggcaccgc
     8401 caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg atgcccgcac
     8461 ggttaatggc gtcattcttg aggcactgga agagatccct gtcgcaggca cccgcgtgcg
     8521 tattggcgag tacgatatag atattctcga cgtacaggac aatatgatta agcaggtaaa
     8581 agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgcaatg gcgaactccg
     8641 gttcgccttt tttttatcgc cgtcttttag gattccatca attcctgtca tttacttagc
     8701 aatgtaataa tgtcttttca agcattcgga aaggatattg atgttatgtc agaagattat
     8761 gtaattgaat gggataagga ttttgcagac gatctcaacg tcgtcgccag cgttttttta
     8821 cagcacaccc caaccttatg gcccactatt ttctctcaac tttcgacgca acctgaaatc
     8881 tttgaagacg aagatgaaga cgaatatgga ttgcaggacg ttctcgattg cagtggtggc
     8941 gatctcggga atcgtgacct ggcgcaggca tttttacagg ttctacgcgg tgaaggatta
     9001 attcaactcg ttgactggaa aggtgaagat gaagaaggtg agctggctaa ttttgctgca
     9061 gatcgctttt atgagctcac aaaaaacctg atagcttctg aggaattaag gagtcttctt
     9121 gtcgaaataa cccaagagga tgaaatctcc gatgtctgtg aagccggaga ccgctacctt
     9181 gacgaaatct ttgaacgcat ccaaaccgaa ctcaataaaa ggggctttca gatttttgat
     9241 ctgaatgaag gatctgacac ctataacgtg gtcgttctgc caatgaacga atataaaaaa
     9301 atagatgatt tcaacacgcc gtggctggaa gtacaggatt ttttaagcta aaaaaatggc
     9361 cgacttcgcg tcggccatca gatatttact tcgccttcgc tacagtaacc atcgccgcac
     9421 gaatcgtacg accattcagc gtataaccct tctgcataat gcccagtacg ttacctggcg
     9481 caacgtcatc agattccacc attgcgatag cctgatgcac attcgggtcc agcggcacgt
     9541 tagtttcggc gatcacttca acaccaaact tacgcacaac atccagcatc gacttcagcg
     9601 tcagctcaat gccttcaacc atcgcagaca tatccgggtt agctttatca gccacttcca
     9661 gcgcacgatc caggctatca atcaccggca gcaattcgtt gatgaatttc tccagcgcga
     9721 atttgtgggc tttttcaata tccagttcag tacgtcgacg caggttttcc atttcggctt
     9781 ttacacgcaa aatgccgtca cgttcacggg tctgggcttc agccagctga gcttcgagat
     9841 tcgcaatttt ttcatcgcgc ggatccacct gctcagcaga agcttctggc tcaactgcct
     9901 caatctcttc gtgctgatcc atgataattt cttccggggc ttgcccctca ggcgttttct
     9961 gttctttact actcatgaat ttctccgcgt ttttttcgca ttcatctcgc taacttcgct
    10021 tattatgggg atcagtttca gggtttcaag ggaagcactc acattgtcat caatcttcgc
    10081 aacaaggacc tcggaaaaat gaataatcat ttcaagtgta ttggcattgt gggacaccca
    10141 cggcacccca ctgcactgac aacacatgaa atgctctacc gctggctgtg cacaaaaggt
    10201 tacgaggtca tcgttgagca acaaatcgct cacgaactgc aactgaagaa tgtgaaaact
    10261 ggcacgctcg cggagattgg gcaactagct gatctcgcgg tagtcgttgg tggcgacggt
    10321 aatatgctgg gcgcggcacg cacactcgcc cgttacgata ttaaagttat tggaatcaac
    10381 cgtggcaacc tgggtttcct gactgacctt gaccccgata acgcccagca acagttagcc
    10441 gatgtgctgg aaggccacta catcagcgag aaacgttttt tgctggaagc gcaagtctgt
    10501 cagcaagatt gtcagaaacg catcagcacc gcgataaatg aagtggtgct tcatccaggc
    10561 aaagtggcgc atatgattga gttcgaagtg tatatcgacg agatctttgc gttttctcag
    10621 cgatctgatg gactaattat ttcgacgcca acaggctcca ccgcctattc cctctctgca
    10681 ggcggtccta ttctgacccc ctctctggat gcgattaccc tggtgcccat gttcccgcat
    10741 acgttgtcag cacgaccact ggtcataaac agcagcagca cgatccgtct gcgtttttcg
    10801 catcgccgta acgacctaga aatcagttgc gacagccaga tagcactgcc gattcaggaa
    10861 ggtgaagatg tcctgattcg tcgctgtgat taccatctga atctgattca tccgaaagat
    10921 tacagttatt tcaacacatt aagcaccaag ctcggctggt caaaaaaatt attctaattt
    10981 tacgccagcc tctttactgt atataaaacc agtttatact gtacacaata acagtaatgg
    11041 tttttcatac aggaaaacga ctatgttggc acaactgacc atcagcaact ttgctatcgt
    11101 tcgtgagctt gagattgatt ttcatagcgg catgaccgta ataactggcg agaccggcgc
    11161 gggtaaatct attgcaatag atgccctcgg tctttgtctc ggtggtcgcg ctgaagccga
    11221 catggtgcgt accggcgctg ctcgcgctga cctgtgcgcc cgtttttctc tgaaagatac
    11281 gccagcggct ctgcgctggc tggaagaaaa ccagcttgaa gacgggcatg aatgtttgct
    11341 tcgtcgcgtg atcagcagcg atggtcgctc ccgtggtttc atcaacggta cagctgttcc
    11401 tctgtcacaa ctgcgcgaac tgggtcagtt gctgattcag atccatggtc agcacgctca
    11461 tcagttactc accaaacccg agcaccaaaa gttcctgctt gatggctatg ccaatgaaac
    11521 ctctctactc caggaaatga cagcacgtta tcagttgtgg catcaaagct gccgtgacct
    11581 cgcgcatcat caacagttaa gtcaggaacg cgctgcccgt gcggaactgc tgcaatacca
    11641 attaaaagaa cttaacgaat ttaatccgca gcccggagag tttgaacaaa tcgacgaaga
    11701 gtacaaacgt ctggcgaaca gcggtcaatt gctgaccacc agccagaatg cattggcatt
    11761 aatggccgac ggtgaagacg caaacctgca aagtcagctt tacacggcta aacaactggt
    11821 gagcgaattg attggcatgg acagcaaact gtccggcgta cttgatatgc tggaagaagc
    11881 taccatccag attgctgaag ccagcgatga actgcgccac tactgcgatc gtctggatct
    11941 cgatcccaac cgactatttg aacttgaaca gcgcatctca aaacagattt cgctggcacg
    12001 taaacatcac gtcagccctg aggcattgcc acagtattac cagtcgctac tggaagaaca
    12061 gcagcaactg gacgatcagg ccgactcaca agaaacgctt gcgctggcgg taacgaaaca
    12121 tcatcagcag gcactggaaa tcgcgcgcgc attacaccaa caacgccagc aatatgcaga
    12181 agaacttgca cagctgatca ccgacagtat gcatgcgctc tcaatgccgc atgggcagtt
    12241 tacgatcgat gttaaatttg acgagcatca cctgggcgct gacggtgccg atcgtattga
    12301 gtttcgggta accaccaacc caggtcagcc aatgcagcct attgccaaag tcgcatccgg
    12361 tggtgaattg tcccgcatcg cactggcaat ccaggtcatc acggcgcgta aaatggaaac
    12421 cccggcactg atttttgatg aagtggatgt agggattagc ggtccaacag cggcagttgt
    12481 cggcaaactg ctgcgtcaac tcggcgaatc aactcaggtg atgtgtgtta cccacctgcc
    12541 acaagtcgcg ggatgtggtc atcaacacta ttttgtcagc aaagaaaccg atggtgcgat
    12601 gacagaaacg catatgcaat ccctgaataa aaaagcgcgg ttacaagagc tggcgcgcct
    12661 gcttggtggc agtgaagtca cacgtaatac actggcgaat gcgaaagaac tgcttgcagc
    12721 gtaaactttt ttcctgcttc acggtcagag taaacagcaa aacgccgtaa gaccggaaag
    12781 caaaaggttt taaagtgatg aaaggtctat tatcatcggc atattacaga tgagccacgt
    12841 actgctcggg cccgaaaagg aatcaaatca ctatgcgctg taaaacgctg actgctgcag
    12901 cagcagtact attgatgttg accgcaggct gttccactct ggagcgagtg gtttaccgtc
    12961 ctgacatcaa ccaggggaac tatctgaccg ctaacgacgt atccaaaata cgtgttggca
    13021 tgacgcaaca acaagttgcg tacgcattgg gtacaccgct gatgtccgat ccatttggta
    13081 cgaatacctg gttctatgtc ttccgccagc aaccaggtca tgaaggtgta acgcagcaaa
    13141 cgctgacgct gacctttaac agtagcggtg tgttgaccaa tattgataac aaacctgcgc
    13201 tgagtggtaa ctaataataa agttgctctc aaagacgtta aaaaaggtgc tcaatgagca
    13261 ccttttttct gtctgttatt tattcgctga tttttctgct cgttgcctgc gaagctcttt
    13321 cggatcggca atgagagggc gataaatctc cacccgatcg ccatcatgca cactatcgct
    13381 tagttttgcc ggacggctgt aaatgccgac tttgttttta gttaaatcga tatcggtacg
    13441 caattccagc aagccactgg cgcgaatagc ttcttcaacc gtcgcgccct cctgcagcgt
    13501 cacgcgctgc aggtactgct tctcaggtag cgcataagcc acctcaacgg caattttacc
    13561 tggcactgta aacctctttc gcacgaaccg taaaagcctg gaccatatta gacgccagct
    13621 ctttaaacac gcgaccaaag gcgagttcaa tcaacttatt ggtaaactca aagtcgagat
    13681 gaaactcgat acgacacgcc tcctggctca gcggcgtaaa cttccatcca ccaatcaatt
    13741 tcttgaacgg cccatccacc agattcataa gaatactttg gttactggtc aactggttgc
    13801 gggtagtaaa cgttttgctg atcccagcct tagagacatc taccgccgca gtcatctgcc
    13861 caggagtgga ctccagaatc cgacttccgg tacaacccgg caaaaactga ggataagact
    13921 gaacgtcatt cactaactga tacatttgct ccgcgctgta gggtaccagt gcggtccggc
    13981 taatctgagg cataacaatt tccatcaaca aaaatccaac aaataatatc atttatcctg
    14041 ctgtaaaaaa aacgctatcc cggcgctggg taacatcggg ttcatgctaa gatagagcct
    14101 tgtcccccgc aggattgata tggggtgttt tcgatttcag attaccgatg attcacgacg
    14161 cttatgacga agaaaaaagc acataaacct ggttcagcga ccatcgcgct taacaagcgc
    14221 gcccgtcacg aatactttat cgaagaagag ttcgaagcgg gacttgccct gcaaggctgg
    14281 gaggttaaat ccctgcgcgc aggaaaagcc aatatcagcg acagctacgt ccttctgcgt
    14341 gacggagagg catttctgtt tggcgctaac atcacgccaa tggccgtggc ctccacgcat
    14401 gtggtgtgcg atcctacccg tacccgcaag ttacttctca accaacgcga actggactca
    14461 ttgtacggtc gcgtcaatcg agaaggctat accgtagtgg cgctctccct gtactggaaa
    14521 aatgcctggt gcaaagtgaa aatcggcgtc gccaaaggta aaaaacagca cgataaacgt
    14581 tcagatatca aagaacgcga atggcaggtg gataaagcac gtatcatgaa aaacgcccac
    14641 cgttaaacct gcactccaat tattgaccag ttcctcaccg cgcctccctc tccggcggcg
    14701 cgaatgaaca tcttattggc tatcacatcc gacacaaatg ttgccatccc attgcttaat
    14761 cgaataaaaa tcaggctaca tgggtgctaa atctttaacg ataacgccat tgaggctggt
    14821 catggcgctc ataaatctgg tatacttacc tttacacatt ggggctgatt ctggattcga
    14881 cgggatttgc gaaacccaag gtgcatgccg aggggcggtt ggcctcgtaa aaagccgcaa
    14941 aaaatagtcg caaacgacga aaactacgct ttagcagctt aataacctgc ttagagccct
    15001 ctctccctag cctccgctct taggacgggg atcaagagag gtcaaaccca aaagagatcg
    15061 cgtggaagcc ctgcctgggg ttgaagcgtt aaaacttaat caggctagtt tgttagtggc
    15121 gtgtccgtcc gcagctggca agcgaatgta aagactgact aagcatgtag taccgaggat
    15181 gtaggaattt cggacgcggg ttcaactccc gccagctcca ccaatcatga ttggacggtg
    15241 taaggacaac accaacaaaa acaggaagtt agcagtctca gcaggacacc gaccagacgg
    15301 tgaggagata aaaaaggata cgcaaaggag ccgtggctct cgagtgacac aaaagcccgc
    15361 ttatgcgggc tttttgtttt tcccttaagt tcctagcctc ttatctataa ctatggaaaa
    15421 atgttaaccc tgactgtatg ttaacaaagg gatgtatatg tcggtttttc ataactggct
    15481 gcttgatatc gcaagcggga attactttat ctacatcaaa cgcctttctg caaacgacac
    15541 aggcgcaaca ggtggccatc aggtcggact ttatatcccc tcaaatatcg ttgaaaagct
    15601 ttttccatct atcaatcata ctcgcgaact gaacccttca gtcttcctta ctgcgcatgt
    15661 atcatcccat gattgccctg atacccaagc acgcgcaatt tattacaaca accgttattt
    15721 tggtaagacc cgaaacgaaa aaagaattac gcgctggggg agaggaagtc cattacagaa
    15781 ccctgaaaat acaggagccc tcacaattct tgctttcagg ttaaacgaac agaacactga
    15841 ctgttccgag gtagatatat gggtctgcgt caatcccgat gaagaggata tcatcgagtc
    15901 tgctattggc gaaatcatac ctggaaccct aatttccggc cctgccggac aaattttggg
    15961 cggattgtct cttcagcaaa ctccagtaaa tcataaatat gttattcctg aagactggaa
    16021 gaagcgtttt ccttctggaa acgaaattat tcaatatgct gctggccatt atgctaaaaa
    16081 ctccaaggat ccagatgagc aactgattga ccgtcggcgt gtcgagtatg atatttttct
    16141 actcgtcgag gaattacatg ttattgatat tattaagaaa ggattcgatt ctgtagatga
    16201 gtttattgca ttagccaact ctgtcagtaa tcgacgtaaa tcaagggcag gcaaatcact
    16261 tgaacttcac ctagagaagc tttttatcga gcacggacta cggcatttct ccactcaggc
    16321 agttactgaa ggtaataaaa aaccagattt cctgtttcct tcagcagagg catatcataa
    16381 cgttgaattt cctgtagaaa acttacgtat gctggcagta aagaccacct gcaaagatcg
    16441 ctggcgtcag atactgaatg aagcagataa aatccatcag gtacatttat ttacactgca
    16501 agaaggtgtt tcttcagcac aataccgaga aatgaaagat gcgggtgtca gactcgttgt
    16561 accatcaact ttacataaaa aatacccaga agcagttaga gaagaattaa taacgctcgg
    16621 agcattcatt actgagctga tagagcttta cgctgaacta tcataggctg actcccggct
    16681 taaaaggccg ggagatgttc tcaaggctgc ccagtcttac cagcatcagc agaaacagct
    16741 ttgaggatat agggttccag aagtctggca acagcttcaa atactggcac cacaactgag
    16801 ttaccgaact gccgatatga ctgagtatct gaaaccggaa tacggaatgg tttccctcca
    16861 ggtttttcaa atcccataag gcgtgcgcac tctcggggag tcagcctgcg tggtcgacga
    16921 gcctgattac tctcattcat aaagtcagcc tctcccgttg ccatatccca accacgatca
    16981 ataagaattt ctgatccgtc tttgtgatat ctggcagaaa gtgtgcgtgc aatgctttcc
    17041 ttattctcag gattgaccaa cccaaagcca aaaccattcc ccttggctgc atgctttttg
    17101 gcgtagttat aaaggtactc ccatagtttt ggcgtaagta tatatttact gtcgactaca
    17161 ggctccagca attcaccaaa tgatgggcgg tgttccggat aaaaacgact gatatcacgc
    17221 aaggtaaacc ccttgtgaat attcagatct cgtctaaatc cgaccagaac aatgcgttca
    17281 cgatgctgag gcaaaaaatg cttcccatcg ataatctttg gatcgttttt tcccatttct
    17341 gcagcatcgg caacttcgta gcccagctcg tcaagggtct ccataatgac tttgaaagtt
    17401 ttacccttat catggctctt cagatttttg acattttcca gcacaaaaat tgccggtttt
    17461 tttgctcgta taatacgtgc cacgtcaaaa aaaagtgttc cttgtgcttc acattcaaaa
    17521 ccatgcgcac gaccaagtga gtttttctta ctaacaccag caaggctaaa tggctggcac
    17581 gggaaccccg caagaagcac atcatgatcc ggcacatgct catcaatata tgcataagca
    17641 tccgtttcca atacatcggt tttatcactc agcgtgactt cccgaatatc gagattgaat
    17701 ttatgcacct gttcatcgtt aaaccagttg gccttgtatg tacgcacagc atctttattc
    17761 cattcactgg taaaaacaca ctggcctcca atggcctcaa aacctttccg tatccctcca
    17821 attccagcaa ataagtcaat gaagcggaaa gcatattccg gatggtttgc aggtggttct
    17881 ggtagcatct tacgcagaag agactcttct actgaagtca acgattttgg taaacacttg
    17941 ccattaatcc agcggttaat ggtttcacgg ctccactcat tttttccgac ttttctcagt
    18001 aattcagcca catacttctg atcatagatt tccagcactt tctcgataag ctttttatca
    18061 ttttcctgtc gcagcttttc ttccgcctcg gcttccttca gcagatgctg tgccaacact
    18121 tcaaattcag acataattcc tccaaggggt ctaatgggtg aaactttatc actcattcaa
    18181 cccagaagga aatgttttat ctggatattt aaacagtgac tacaacgtaa tctagcactg
    18241 gtgatgcttt gttaggcata gagaatcatt ctatatacga ctaatgacag aaaaacagca
    18301 gacaagtagt ttgttcataa attaacgcat actatgtgtc tacggttttc gagaccggtc
    18361 caatcatcaa acgaaacata aaattagctc acattatgag gaaaagtatc ttttttgtac
    18421 tatgtaaatt caaaggctta gcctcatttc tccgatggtt ttctcaacac tactggttgt
    18481 gagcccttgc aatgatcatt aatatacgtc tcacaaataa ttcttcatag atattgcaaa
    18541 atggatatta ctgagtttcc ttctggagta attgaacacc ttggctggta tgtataccga
    18601 ttgattgatc cgagggacgg aagcaccttc tatgtaggga aaggcaaagg taaccgcgta
    18661 tttgcccata tgcgcggtga agtggcagcg actgatgatg acgagttact gagcaacaag
    18721 ctaaagcaaa ttagagaaat aaggttagca ggacttgaag ttatccatgt catccatcga
    18781 cacggaatga ctgatgaaaa gacggcgtac gaagttgaag ctgcacttat tgatgcctac
    18841 cctgggttaa cgaatatcat gaatggtgct ggcagcaatg aattcggcgc cgcgcatgtc
    18901 aaagagttga tagcaacata tcaacccgaa accataatat ttcatcataa agcattaatg
    18961 atttccgtta acagaagtgc aaaggattca gagctttatg atgcggttcg atttagctgg
    19021 cgcattaatg tctctcgcgc cagccaagca gaagtcattc ttgctactgt aagggggatc
    19081 gttcgagggg ttttcattgc tgataaatgg ctcaaatcaa cacgtgaaaa tttccctacg
    19141 atgaaatact gggacgagga tcctgacttt gaggcaacac aaagttctcg ctatggtttt
    19201 gaaggtcgag aagccccacc tgaaatagca aatctttatc ttggaaaaaa aataccagat
    19261 gaattaagaa aaaaaggagc tatgtccccg gtccgttact cacctaattt ttgagtcttt
    19321 aagtgataag cataaaccgc agcacgatct tgcatacgac gtgctacggt ttcatttatc
    19381 tccgaccgaa aacttcttat acagtgtcga tataccaaca tcatagatga tcgccacctt
    19441 ctggcgagga acgcctgatg caattaatcg cccagcctgc gcccattgtt ctggtgtaag
    19501 tttgggacga cgtccaccaa ttcgtccctg tgcgcgagca gcttccagtc cagcttttgt
    19561 tcgttcaaca atcagttctc gttccatttc agccagggca cccatcacat gaaagaaaaa
    19621 acgccccatc ggtgtgctgg tatcaatagc atccgtcagg ctgcgaaaat taacgccacg
    19681 ttcgcgcaac tcctcaacca gaatgaccag atgccgcata ctacgcccca gccgatccag
    19741 cttccagaca accagagtgt caccttccga taatgtcctg agcagttttt tcagtcccgg
    19801 cctttcagac tttgtaccgc ttatcttgtc ttcaaaaatc agctcgcatc ctgcacagtt
    19861 cagcgcatta cgctgtagat ctgtgttctg gtcatttgtt gacacacgta catagccaat
    19921 aagcatggta gatctccctg acaaaagcag gaatgatgcc atttgctcgt tatttctgca
    19981 ttttcataaa cgttggtttg ggagaagcgg cgaaaaggga tgtaggcaca ggagaaaatc
    20041 agataccgga tatgtcagcc tggaaaagaa atccgagttc taatcgctgg agaaaattgc
    20101 ctgatggaac catcattcaa atgggaatat cagcatcagg gccattaggc tcacctgtaa
    20161 atatcaccct gccgatatct ttcagcaata ccaattattg tgttgttgca tcgtacgata
    20221 atgcacggtc aggtgtgtca acaatggtta gttttgcagc attacctgtt tcaccatcgc
    20281 aattttccct gatgtcatct gtgactgagc aaggaataaa tccttttgct tactggattg
    20341 cttttggaga ttgataaatg gacagatact tctattcaca aaaagaaaat ggttttttta
    20401 ccgatttaaa taaagcacct tcagatgctg ttgagataac cacggatgaa tggctgtcac
    20461 tactggatgg tcaggataat ggcatgaaaa tagtcagcaa tcaggaggga tatccggttt
    20521 tgacagagca accaccttta tcaaaagaaa accttattgc attggcagag ttgaaaaaag
    20581 gaaaacttat taatgaagcc aacgagcaca tgaacagcag gcaatggcct ggtaaagcgg
    20641 ctattggtcg tctgaaaggt gaggaactgg cgcaatataa tttgtggctg gattatctgg
    20701 acgcactgga gctggtcgat acttccagtg cgccagatat tgaatggcct acgcctccgg
    20761 cagttcaggc cagatgatat ccggcgcggt gctggtatct gttaccgtca ccgcgtcaat
    20821 gtaatccagc acggcgttaa gtcgggttgt ttctgcctgc gtcagtttcc gtccggcctg
    20881 caatttcagt tgaatcagac taatggaagc cattacagta tcaatcagtg actgacgttg
    20941 tgcttctgcc gcgtctactg cggcgctatg ctgtgcctcg gtatccgtca cccatttctc
    21001 accatcccat ttatcgtatg gcgttaacgg tgaaagcgtg acataaccgt ctttgatggc
    21061 accgatataa tccactgtaa cagctgcgcc attttctgtt gagtaaacag tctcattgcg
    21121 atggtcttct tcatggctcc atcccttacc cgtaaatact gccacttttc ccggaatgtt
    21181 ttcgccaggg tcaataccag tggaacaggc gggcatactt acgccagtat taatatattc
    21241 atcagaccag cccgtatact cagatgtttc agcatcataa taaaaacaac gcatatcgcc
    21301 cggcactgta gccagcccat tttcatcaaa aacaggtttc attatttagc cctcaccaga
    21361 aagttaaatg caatatttcg cggtctgaca gcaacaaaat tcacaccatc acccacagag
    21421 ttactggtga aattaaatcg tgaaaatcct ggctgatttc cggcgatgcc atcatgaaag
    21481 ttaattgcgt gtcccgcacc ttcgcctata ttcccggcaa actgagaaaa gtttgtagct
    21541 gcctgccagc ttaataattc acgaccacca tctgcacctc gcccgtcatc ccagatacga
    21601 atgaaatcac cgcgggcttc aggtaatacc agcgaaggaa acactttcgc cagcacagga
    21661 taatcagtgg cagagaattt cgcgccgttg aacttcaaaa acaccatact ggaccagctg
    21721 tcgattacag tatttggcat tgcagcggac ggccagaaga acggaacgcc aatagctgga
    21781 gaaccttctc ccaaaccaag gttttcgaga gccgttttca ccgtgccatc cgatttgata
    21841 tcaccaaacg gattcttgcg gcttaacagc agcgcacgaa gcgcggtaag cagctggtcg
    21901 tgccgcccct tctccaggct ggcaccggac gcctccacaa cgctgcaaag ctcctcctgc
    21961 aacatgtcaa agtagtcatc atccagatcg gtggcaggcg tgccggtctg ggggttacca
    22021 cgggtaaaac cgttcttacc cgcgccgaac ttatccttct gcgcggtttt cgtgtctata
    22081 cgatgcatgg attactccgg atatttaaaa attacgtagg tatgcgacgg gcagagtttg
    22141 ttaagcacac actcgacaac ggtgtcgccc cagatacgca gtgcggaatc acagggatcg
    22201 ccacatgtca tccaggtggt gttggtggcg gctggcatgt tgacctgcca gtaataccgc
    22261 cattcaggcg cgttcacagc gtcagtacag gccgatgagc aggtgaacgt gcttttgtcg
    22321 tatcgcgtga tggtggcatc tggtctgccc aaggcagcaa gctgtgcaag ataaaaatcc
    22381 tcgttgatgc cacccgacag gttaaccttc gcatccagcc gttgctgacg ctggcgaagg
    22441 gtctgtgtcc ctgcgggaat acattcatcc ggcagaccgc acagacgctc ccagcggttt
    22501 atcagttcag tggtagtgcg cggatccagc tcccgcatca gggcatccgc acgctgatga
    22561 acgcgggtta atgacggtgc cgcaccggca atcgccggat cgctggctga ccacgccgga
    22621 ccggggggca acagtgccga caacagacgg atgtaatcat cgtttgtcac gtccatgaaa
    22681 tcgtccccag taccgccagt tcattttttg caatggagat attgtctgcc ggtgcaagca
    22741 actgatggct gtattccccg ttcgcaccgg aaatcgcttc actgatacgt gacaccttca
    22801 gttctccctg cggataacca tcacgcagca ggaacgaacg caactcggcg gtgatggcag
    22861 cccgtatttc cggtgtgtcc ggcgtcacgc ggatatgaaa atccacttta tgcgccaccg
    22921 gcctgaatac atacaaatca gagcctgcca ccggggccag tggcccgata tgttgtcttg
    22981 ccgccgtttc cgttgattct tccggaatgg gattaatcag gtcactgctg gcaatcatca
    23041 caccgacagt tcccgttccc atccagtgac ggtatgtcca tgcgcgggta atgccgggca
    23101 cttctttagc ccagacgaca tagtccccgt cagccccgcc ctgcggcgtc cagtaatacc
    23161 gctcaatgac gcgggcgcgc cacgtttcca gatcttcagt atcgaatcct ccagtcaggg
    23221 tatctgcaac accggaagac ggcagaccat tcaccggcgt gaccaggatt aatgccgtac
    23281 cgtcgtcagc gttaccgacc gcgcctgtag ttgagcaagt gatcggcaca cgcaggacac
    23341 caccggagct ggttgcatcg gcagttgccg tgtactgaac caggtcatcg cgctgaataa
    23401 cactcccggc agtcaccttc aggccatcgc tgacaccttc ccagcgcata tacccgctgg
    23461 cagccgtggc ccccttgcgc ggacaccgtt tcatcgcagc atgtcgcgcc agccaggact
    23521 catcgcacag gtcaggcagc atattcattg ccagataatc gatgtacccg taaaccgtat
    23581 gcagcgccgc cgcatacacc tttgcccgca cgtcttcatc catgcgccgg agcgtgtcgc
    23641 tgacgtccag cctggcaaat aaatcgttac ggagcatact gatattttct gccagcgtcg
    23701 ggcgctgaaa ttcactgtcc gccatgcgtt atcgcactcc acagatcatc aaaagaaatc
    23761 attaccggtc cgtcacgacg ccagagagtg atactgttac ccagttcatt aatcccggtg
    23821 cggcggatat ccagatcaat acgggacacc acgccgtcat cagtcatcca ttgcaggcat
    23881 tcgcggatat acccccttac cgtctgcacc agctgattgg tcagtttgct gcgctgaagc
    23941 agccacagtc gggagccgta acggtcattc tgtaccgcag gccaggtatc cccccaccat
    24001 cccatcggga cgtcggcgtt gtcatcaggc tccgcccgcc gccaggtaaa cagggaaatc
    24061 accacggcgc gggtcagcgg atccagcggt gcgctggcgc aggtgcgttt accgttcacc
    24121 gtcagccaca gttccatcat gcctccatcg ctttatcagg tttgtcggtg ttactgccct
    24181 gaccgttctc tctgtgacga tgcccgttat aggcaagccg catcgctgac atggtggtgc
    24241 cgccggagtc gcacaggtct ttcacctgtc cggtcacttc caggtccatt tcaaaacgtg
    24301 ctctgggcgc attgcgaaac gtgatcgttt tacctgcacc gtccaccacg atcccctccc
    24361 gggtcagcgt cacggactgc ccctgatcgt catagacagc cacctcaccc gtctgcagcc
    24421 ctttcaggcg gtagcgccgg tccgacaccg taacaaccac cgcatgagaa cggtcgccat
    24481 ccggaaacaa caccaccgct tccgcaccgc tgtttgccct tgcggtaaaa ccgtagggtt
    24541 caagatgttc aaccccggct ttgggttcac cggcaatcag ggacacatcc acggtctgac
    24601 atttcgtggc ggcactgatg cttttcacca cggcccgccc aatcaggccg aggagttgtc
    24661 gctgcatggt ttcaatcgtc ctcatcagaa cgggtcctcc tgtactctgg cttttttctt
    24721 tttccgcgcg ccgggggctt cgggttcagg cagataagca tcaggtgggc cgacacggat
    24781 ttccgtctgg gtgccgttct ggtcctgagt aaacgtgact tccgagacaa gcagttcggt
    24841 attgtcgaaa ccacagaccg gatcgaagac aatcacccgc tggttgggct gccacagcgt
    24901 accgttaccc tgtcgccagc cctgcaccac ataggtggtt tcatccgtcc gcgccgcccg
    24961 ttgtcgggct tcaaagtcag cacgcgcaat acaacctgcc cccgtggcct gccctgtctg
    25021 cctgatatac atcggacggt aacgggcaat aaatgcgtcc tctgtgcgtg cccgcagcgc
    25081 ggtggtggtg gcctcaccga aatcatcgtc gtttccggca cgctgccccg ccacctggta
    25141 aacagaaaac cgctcccgga tactcttctc cgtatcacag gaaaggatgt tttccccaag
    25201 taccagcgcg gtatgtgccc gcgttgagcc aataccgcca atcaccagcc tgccgtgcgg
    25261 gtcgtcgtaa gccagcgcct gctgctgacc gagtattttg ttgatcacct caatcaccgt
    25321 ttcaccgtga tcaggctgaa catcaggaat aacacccgac ggcgcaccgc tgttcaccac
    25381 ctcaatgccg aaaggcgcag caagcgcctg cgcaatctgc accagcgagc gtccgttaaa
    25441 ctgtgtcggt tcggctgcac agtcaatcag gtcagccgtc agactacgtc cggcaatacc
    25501 ggtgctgacc gaacgggcat cgtaacgaac gggagtcgcc tccacccagc cggtgatcac
    25561 cagctcatca ccaatcagca cttccacttt tgaaccgttt ttaatgcgcg gctgaagcgt
    25621 ggtgataccc tcatctcccg gccactggcg agtgatctcc acactgaaat cccgcgccag
    25681 ccgttcaata ccggcaccga tgcgcaccga tgtccagcca ttccactccc ggccatttac
    25741 ccgtagcgtg acattgtcgt tcattgcact ggcaccttca gagggatcac cggcacaaag
    25801 ccgggatgtg taatggcatt acgccggata atgtccgcgt cacgcgccgc gttatcaaac
    25861 caggtcgccg ccagcaccag cgcgggtaaa acctcatccg gcgtgcgctg aatgatccgt
    25921 gcagactgtt caaggcgcgt gttgatatcc gcattcagat ctgctttcac ccggcgcagc
    25981 gccagaaaca gcgcatcact ggttgtacgg gacaactcct tatcaattgc cgtattcagt
    26041 gtgtcgcgaa tgtcagtcag ttcttcccac gtcggcaggt caaccgtgtt tttcaccgcc
    26101 ggtgcattgt tcagtgccgg atgcgtgacg gaaggccagc cagtgctctg cgcgggtgtt
    26161 gttgcctgcc ccactgcggc attctgcatc accgcggaag ttgttggcgc aggcaatcgg
    26221 gtgacggcat acgccgcttc gctgattgcg gtcgtacgaa ggatgctggc aaccacgtta
    26281 cgctgctgcg tcgccgtggc ggtggtttta ctgtccgttt tccagacacc gcgcggttgc
    26341 agatcgctgc cgaggctgac accggaaagc gttttgatca tggtgaccag gtcgctggcg
    26401 ttaccataaa ggcgtttccc ggtacgccac attttctgca cctgctcaac gaaatttttg
    26461 cctgacgatg gcggcggcag aagtaccgag atatccccct gcaacagcct ggcggcatcc
    26521 gatacggcag aatccaccac tttcatcgca tcagaaacat accccagcat tgtgccggca
    26581 ttaccgacga cgtcgttctg cacaaaatct gccacgccat cgatactgaa accactgaag
    26641 ctgtcactga tgcagtcatc cagtgcagaa caggatgaca tcagcgtctg cgccgtcgcc
    26701 gcacctgaag tggggtaaga gagttctccc gcttcgacaa acttcaggtc aaagcggaca
    26761 atacgccctt cactcttcga tgtgctgacc cgaacttccc cgtcaacaca gactttcagc
    26821 tcaccgtaag tcggatggac aagcgtgccg ggaccgggtt tattcagcgc gtcaatcagg
    26881 cgatcgcgct ggtcaaagca gtcatctccc accacataag ctgtgatgga cgggcggaaa
    26941 gtgattttcc ccaggtcttc ggtatagggt ttgtcgcggt tcgggtattc gtgcgtttcc
    27001 acacgacgac cggttcccgc actttcttct tcaaccttaa acggcacacc gcgaaatgac
    27061 gcgtcctgaa gtctgtcttt ccacgtcata taaactccgt acataaaaaa tcccaccgga
    27121 gtgggactca ttaacagatt aatttttcat tacctgccaa agcgcgtata gccaacatca
    27181 tggctgacat caaaaccgct ggatcgcgtt tccataaccc gcatacccgg aggcgaattc
    27241 acaaaagaga ccttgatctc accatcaact tttggcgcag aagctttgtt aatcatgaag
    27301 ggattcgggc ctgtggcatc ggaggcgttg tttgactgag ccggatctac cgccggataa
    27361 ggtgtgtatc cccgtgccgg tattcccgtc ccataagcat cataagcacc cgccccccac
    27421 tgcgccgagt taatggcatc gaccgtgtca ccggaactgt cggtaaacca ctcaataatt
    27481 ggcttcagct tgtcccacat atcctgaaac cacttaacaa ccggccccca gttattgatc
    27541 accatcccca gcggcgacca ggcaaaaact ttcttaagga gttcccagcc agtctcaaaa
    27601 taaggaccaa tggtttccca gagtttctta aaataaggtc cgacaacatc ccagttagtg
    27661 ataattaatc ccgcagccag ggctatcgcc gtcgcaatca tgccaatcgg cgtcatcgac
    27721 atgagcctgc tgacaatact gatggcactg ccaacgccca tcaatcccag tttcagaatc
    27781 gcaagaccgg cagcaagccc gacgacgccg cgaataaccc ggggattttc atccgcaaac
    27841 ttcgtgaatt tttcccccaa ctcccccagc cattgcgtga tatttttggc gtcaccagaa
    27901 aatgcgccgc caatagccgc aaggccgtta gttgcggtcc ccgtcattgc ctcccacagg
    27961 ttggacagcg taccaagctg ggcctgaaca cgtttattca ggctggcctg tttattcatc
    28021 ttctgctgga tctgatcgta gccatccttt cctttatcga ttagtgcatt gaccacctga
    28081 agggtttcgg catcatcacc aaatattgcc ttaagtacac ctgtccgctt aacgtcggtc
    28141 agttttcgca gctttgccag ttgcctgaac atgttatcaa gaccgccaaa acttcctttg
    28201 ccgtcagtaa aatcgagctg taccccgagt ttctggcggg ccatgatttt attgacgtcc
    28261 ctgattttct taacgcttaa tccggactgg ataacttttc gcagggcatt acctgccgac
    28321 tccccgttca tccccatctg atccatcatg acgctgatgg gggcaaggct ctgtgcagcc
    28381 tgaagaccgt ccttgttcac catcttcaga acagaactgg ttttagtgaa gaaggacaac
    28441 atgttggtat cgtcaacgcc cagataaaac gccttctgga tagtgtcgaa cagccccatc
    28501 atgtcttctg acgccgttcc ggtagcatcc tgcatctttg cagcaaactc agcagccgct
    28561 tccggtgttt ttttcagttg taccgcaaga taagctgtcg ctttacccac accgccaaga
    28621 atgttttctg ccgggatccc ctgacgcacc agcatctgca tcatgttctg gaaatcagcc
    28681 gttgtaccgg gaagctggtt acccaggcca atagccagtt tattgatgtc ctgaaagcgc
    28741 tttccaacct cgccattcgc atccatcatg gcgactttca gcccggtggc ggcgttttcc
    28801 tgatcggcat aagatttcag ggaaagcgtc agacccgctg ccagtccgcc accaagcgcc
    28861 agcccaccct gtgacgcttc ttccgcctgg cgtttaaatc cccgtatttt cttttgcatt
    28921 ttcgacagcg cgggagaaag cctgtcgaca ccggtgatca acgccttaag ctcaaattca
    28981 gccatgtgtg cgtttctcct gctctatcct gtttgcctga ctgaccagca agggaatttc
    29041 actgatcggc atattcagca attcgaaagg attaatgcgc cagtagctgg cgcagtcaaa
    29101 gaagcgatca gtaaggtatt cagccgtcag gcctggagga aaaaaccagc cacaagccac
    29161 gccgctgcat tcaggtctgc cggagacatc tggtcgacag agctttgcgg cactttcgcc
    29221 agccgcacaa tatatttcga taccacatgc gccagaagtc tgacggactc atcctgattc
    29281 atctggtagg gataccccag ctcgcggaca tctttcccgg tgggctcatc aaactccagt
    29341 acggagagtg tctcgccatg agcggtaatc ggtttcttta actcaagctc tttcattact
    29401 ggtaatcccc ttcttcaccg tggaactcaa gatcgaccgt gccttcttcg gcattatggt
    29461 tcgcttcgcc gtgcagccaa gcagacgaca gtacatagac ctgaccgttc gccagctcgg
    29521 cagtgattgt catctcatca gacgaggtga ttttgcccac cggaaaattc ttcggcacct
    29581 tgaaggtccc tttgacataa ggcgcacggt gagtttcctt gcggtccact gaaccgtcca
    29641 ggccgatgat gtcatcatta accatcctgt tcatgggcac ctcaatgccg ccggtcagcg
    29701 atagctgctg accgtcaatt ttgaaataac aggttccccc gatacgggcc attatgcaga
    29761 ctcctctgaa tactgaagac ggaactggtt aaccacggca aagacacgca actggttaac
    29821 atagtcaggc gggaacagcg tgttcaggcg gttcggatcg ctggcatcac gctccacaac
    29881 caggtactgc ttaaaaagtt cgtagttttc cacgatcccc gcacgctcaa gctgacggta
    29941 ggttgccagc agttcccctt tgatcaccgc cggggtgaca atcgcctgac cgggaccaaa
    30001 gcgggtaccg tcgctggcaa gcttgtgacg cccgtactta ctggtaatga cggatttcag
    30061 tttgcgcagt acatacgcac tggtatgcag cgtctcgctg tcgaggtagc tgttatccgc
    30121 aaccccgtaa gcgtttttcc tgtacgtggt gacatcacgc tgaatgcgca gtaccccgct
    30181 ttcgacatac gccgttgcca cgccatgaga cagcagggtc tgttgttcgg tcatcgtgaa
    30241 ccgtttcccc ttcggcgcag acagcatacc caccagctca ccggtctgcg tgggacgtgc
    30301 cggatcgttg cggataaaca ccgctgcgcg ggcggtacgg cttgccgcca gctcgtcggc
    30361 aggcgtctgg gtgtcttttt cgtaccccgc cagggtaatg tgctgctggt taaactggtc
    30421 acctgcggtc accagttctg acagcgtgcc gatctttgcc gtatacacat gaccatacag
    30481 ctgacgcgca tagctccagc gaccgctggt atcgttcatc tcggtcacca gcgtgttaac
    30541 ggaggccgtg tcgttgaacg gcaggccgat ataatcaaac ggctcatccg ccattgcagc
    30601 caccgcgccg gtgagaaccg gagcacccgt accggcggta cccgtcgcca cggcaatctg
    30661 tacgcccgct ggcagcactt cgcccccacc aaagccgtag taattgaggc tgacaggaat
    30721 ttcattcccg caaagcccct tatgacgcgc ggtcagtgtg accacgcctg ccgaagatga
    30781 agccgtaaac ggcagggccg gaacggcatt gatggcatct ttgatactgc tggcaatcgt
    30841 cgtgacgtta tcgccgttgg tcaccggagc ctgcacgcgg gtacgtccca cataaacatt
    30901 caccgtgccg gtttcggttg ccgcgccggt caccgtcagc gtaactgttg ccgccgcgcc
    30961 cgtggattca ggaacggcaa tcacatacag ttcaccaaac gggtcggtct ggcgataagc
    31021 ctcgaccata cgcgccagct gacttcccgc accacaaatc tggcgtgcat agtctgccga
    31081 tggcatcagc accagactgt tggcaacaat ctctgcaccg ttattggcat gaccaatcag
    31141 cagcgatgct ccgctgtcct gtgcagtatt cgccgcctgg ttatccattt ccgcataaaa
    31201 aatcggaacc agcgtattcg acggaatggt gttaaagctt atcgtcatcg gtattcacct
    31261 tttcattcac gcgccggata tcacccgctg cttcacggcg cagccagtag ttgttctcat
    31321 caacatttcg cccctcggcg ggcaaaaggt caccgcgggc agggtcagga actgaccgcc
    31381 ctttaacagg tttcacaaac atgaagattc tcaggaagga agggttattt cggtgtgatg
    31441 ttcgatatcg ccgtcaggcc cgttaccggg atcgagataa tcaacatcaa tcgccagcgt
    31501 tcgcagttca tccagactgt tcaggtcatc ctgctggcgg gtatcgtctt cagtcagctc
    31561 gctgatgacc gaaaaatcga actgataaat cagctcatga cgattcagat ccagcagcgt
    31621 gccgccgtca taggtaatcg ggttaccgca cgcctccggg ttccagccca gcagagcctt
    31681 aaagagcatc tgccggacat cgtccaccac atcatacgag gcaaactgac cgcgctcatc
    31741 acgcccgtta ctcagtatga caaccacgga gaaaccctct ttcagctcct gccagtagtc
    31801 ggtctggctt ttgttttctc ccggagaatc atcacccggt acaacatatg ccgccgggag
    31861 tttcagcttt ccgacctccg gcagattttt gaactgggcc gcgcctgcaa cccggttttc
    31921 aaaatacgga caacgggcac gcagtgcagc aataacaggc gtcagtttca tctgtgtcgt
    31981 cgctccggct tcagtgattt acgcaattcc cgcgccagaa aatagcgtgt ccagctgcgg
    32041 ttcttttcaa gagtttccac catgaagtta ttacgtggag ccagtcgcca gccgctgcca
    32101 ccggatgcac cacgatgatg gccgcgacga cgctttgccc ctcgcctcac gccatagaac
    32161 aaaaaagccg gataaaaatc accggtgata cggcggtttc cctctccatt acgctggtta
    32221 ggggctatac gtgccataaa accagggcga tgtttactgg ctctgggtac catgtaacca
    32281 attgaacgag ccaggcgtcc ggtctgataa ccggggtttt cacccggtgc cgaccgtgca
    32341 cggcgcatca ccagccgacg ggcatcacgc atatgacgct gaccaatcgt gacaaacgcc
    32401 cgccggacac gggcgcggtt aaagcgcatc tccgcgggct gctgaaaatc aacgtgcaaa
    32461 aaggaagtcg tcattgttgc ctccgtgact ctgcctacat tcgcccagct ccgtacactc
    32521 cagcagcaga aagcgccgcg ccccgttcag atcgcgctga cgtttcaccc ggtacacact
    32581 gtcaccgcag accacctcat aatcagcggt gatcccccgg cggtaacgaa tggtgatgta
    32641 atgggtgatg gcgtccccgg tctgcgcggt ttcctgccag gtggtggcac tggtctggat
    32701 aaccttcgcc catgtccgga acgtaaccgg gtattgaggc tccacgccaa agttatccgc
    32761 gggcatatcc acccgctggc ggatcaggac gcgtttattc agttcaccgg ggtccggcag
    32821 aatgtaggtt gcgctggtct gcgcctgacg aattttcatt gcgggaaata cctgtacggg
    32881 ccgacaagcc agttaaagct cattggcaac tccattttct caacgtctgt aaccgacgag
    32941 cgattttcgt aaaaatggct gataagcatc agcatcccca gacgaatatc atccggcagg
    33001 tgcagcccgt ccggatcgct gtccggaatg gtttcatccg gagcatagag cttccggttc
    33061 agatacgttt ccgtccgctt ttgcgccgca caggccagca gttgcagatg gcggtcatca
    33121 gcatcgaaat cctcatccag ccggagttgg gctttaatct cttccattgt cagaagcata
    33181 ctcagccctc tttactggtc gtggcttttt tctcttttgt cgctttactg ctttttgcac
    33241 tggttccgcg ctctgctaac ccggcctgaa gtgcaatctc ctgcacccgg gcaggaagcg
    33301 ccccgtcgtc atactcaccg gcccgaatga cctcaacacg cataccgtcc ggtgaccatt
    33361 tcagatcttg tttcaggatc atgattcttc acccgtcaga acagggggcg cggttccgcg
    33421 cccctgagtg attacgccgc tgcaatcttc agcagtttga tggcctgcga atcgaccagc
    33481 atgccgccgg tgcgcttggt ggtataaaaa ccgacaaacg gtttattggt gtacggatcg
    33541 cgaagaatgc gggtaccgat acggtcaacg atggtgtaac cccgtttgaa gttaccaaat
    33601 gcaatggctt tcgcatccgc ggcaatatcc ggcatctgct cgttttcagc gataccgtaa
    33661 cccgccagag aggatggctg ccccagttcc agcccaggac gccacagata gttaccctcg
    33721 gtgtctttca gcagacggat ggcaaacagg ctgttgttgt tcatcatgaa cttcgcgcca
    33781 gtgcggtgtg ccttacgcag cgtgtaaatc agtttgataa tggcgtctgc ggtcaccgcg
    33841 gtcgcttcgc cggatacaat atgctgaagt ttgccgaacg cccggacctt gtcggtttca
    33901 tcagtggatt catacgccag gaaccctttc ggcttcttgg tgccatcgcc tgaggtaaag
    33961 gcaatttctt cctgttcggc aaattcggtt gccagctcgc tgttgatcca ggcctccacg
    34021 ttgaagaagg catcgtccag cattttctgg gtagcctgcg ggttgccgta aatttccccc
    34081 atgagaggtt caatcagctc cagtctggag gtggcagtct gggatcgcgt atccgtttcc
    34141 cccacccatc cggaagccgt accgcccaga ttcaccagtt ttttgtagtc ggaaccgcca
    34201 acggtgatca ccgtggcttc ctgacgcatc accacttcat ctttcagcag gttaagaatg
    34261 ttgcgatcca gttcttccgg cacggcgtag ccaccgtctt catcggtacc cacctgcaat
    34321 gccttacgct ccagatcgcg cagaccgtct tcacggcctt tacgcaggaa gcccacaaac
    34381 gcctctttat gctcggtggc cagtttattt tgcgcaccac ctgccggacg tttcagctca
    34441 agcagctctt tttcaaggtc gcttttgaga ttttccagct cgctgagttt cccgttcagg
    34501 gtttccacct gcccggcaag cttgcctttt tcctgctcaa tcgcatccac gcgcttgtcg
    34561 ttctttgctt tgaagtcgtc aaacttctgc tgcagctcct gcgcgacctg ttcgacatct
    34621 ttaatatcaa ccgccatcgt atttctcctg attagaagtt cagatttttc agtgcattca
    34681 gtgcagagcc cacatcctca gcgtcgcgca gggacagtgc gccatagccc ccggccatga
    34741 atgctttggc ctgggtacgg gagagtccga catcacgcag gactctttcg atttttttct
    34801 gttcggggat ttccccgcgg gccagtgcgt tcttgacgtc gctgatccgc gcctcgtcgt
    34861 tagacgggaa cgtcaccagg ctgacttccc agaggtcgat ttctttcagc agaaaggctt
    34921 ctttgctccg gtcgtattcc cagtctttca ggacgtaccc aatagaaagg ccggttaacg
    34981 aaccggcctt catgtgtgca tgtgcgcgtt ttgcgagggg atcatcatca ataagcaacc
    35041 gtcccctgac gtaaagcccg acatcgtctt ccttcatttc ggtgtaaaca ccgatgggtt
    35101 catccatgcg gtgctgccag agcagcgcag gtaacgcttt tctgtcactc cacgcccgca
    35161 gggaagcagc aaatgccccg gacatcacca catcatcgtg gctgtccttt acaccaaaga
    35221 cggagccata cccttcaaac tcaccggagt cactgacaga tttcagactc agcggtacat
    35281 caagacgttg tttcgtctgc attggcgtta tccttctgct taccggcttt actgccatcg
    35341 gagggtttcg tggtcatgtt catcggtgtg agatagacat caccaccggg acgcggattc
    35401 atatcttcca ggtcgcggca gtcattggga gagtaaattc cccagttgat cccggtggcg
    35461 taggcttcaa aacgggactt catatccccg cgcagtaacg ccccggcgtt aaatttggcg
    35521 taataaacgc cctgcttact ttttcgtacc agtccggtgt tgatccgctg ttcgatgcgg
    35581 gtcagatacg gcaccagtga atagttgata aatcccagcc ccagctcttc gatattgttg
    35641 aaggtggcgc gatcggtgtt ctgcaccatg tgcaacggca cccggaacag acgacagatt
    35701 tcttcaagct gaaacttgcg ggtttccagg aactggctgt cctcggcgtt cagcgccatc
    35761 gacttccagt ccagccccat ctcaaggatc atcgggcggt gagcattgcc aagcccggtg
    35821 tgacgctcct caaaatcttt cttcaggcgc tcataagcct gatctgacag cgtctgctct
    35881 gtacgcaaca cacccgacgt caccgcgcca ttgctgaaca gtctggcccc gtgctcttcg
    35941 gtcgctgccg ccagcgatat tgcctcgcgg gcataggcga tgggattcag ccccaccagt
    36001 ccgtccagcg tcagcgtgcg cacatgccag atatcctcct ggctcagtac atccgtggag
    36061 ccatccggga atgtgacctg atagatcggc tcccagctac tgttaagctt cggttccaca
    36121 cagccgggat cgacgggcag cagttcagcc acttcgccaa atgctttcac tttgtaggcg
    36181 taaaagtttc cccgcaggca cagacaggtg accaccagct cccagaactc ctgcggcgtc
    36241 atatagccat tgggatgcgt ggagatcagt ttatgcagac gttcgccggt ggctctctgc
    36301 ttcaggctgc cgttcaggtg atacagattg cagggcaaca tcccgaccga ctctgccagc
    36361 actctgacgc aggaaaaaac cgccgtcagt cgcatggccc gctgactgct gatctgcttt
    36421 ccggtatagg tgtcgtagga caacccgatg gcatccgcca gctctgctgg cgtggtcacc
    36481 ggtgcgtcac tttttcgttg aaataatccc gaaaagaaca ctatttacct ccaccaacag
    36541 acagctgtgt acgatcgaga tatcgcgcta ccagccacga ccagaacagg cacaacgccc
    36601 cggcaacaac aaaccccgcc gggggataaa tcagccaggc accatacgcc agcaaaagcg
    36661 cccccagcac gcccaccaga ggcgcgagaa tcagcatgat cataattacc tcagttaaag
    36721 cgagcggatc ccataggact caatgtggtc agacagcgtg tcttctttct cgtacagcat
    36781 ggctctgcca accgccataa tcagcgcaac tgcaccgtca attttgtttt ccgcctgctc
    36841 tttgacgggt ttcaccacat catcgttacc cggaatggtt ttgccgacca cattgccgat
    36901 acaccaggtc atgatgggat tgccatcatg atgaaagcgc cccgattcaa ttgccgcttc
    36961 cagctctttc atcgggtcgg acatgttggt gtagttctga atgatagtga cgggattcag
    37021 gtcttcatca gcaaggtcat gtgacaaccc ggtcgccccg aaggggtcga tgggtgactc
    37081 actgaccggg ctgattttgt tcgccgcttt ggcctcctcg aggatgtagc gataatccac
    37141 ctccgcacca tcggtaacgg tcagaacgcc catttccacc catttctgaa agcgttcggc
    37201 tgtccggcga tcttcatttt tctcgacgct gtacaccgtg tcatacggta cccagaagcg
    37261 cggagccaca ctgtagtaat gcgttttacc gtcaatctcg cgggtataaa gtcgcgccat
    37321 gctgttcata tccagcttac gcgccaggtc aaaggccaga atgcacggct gcccctcgaa
    37381 ctgctcaagg gtcagtgatt tatcctcgca gctctgccag ctcaccaggt tgaaatacgc
    37441 cgaacgcgcc gacacccaga tattgaggtg ttttgtttta aagacgtttg ccagacgggc
    37501 gttatttttc gcacgctgct gctgacttaa caaaaattcg cgataaaccg acacgccaat
    37561 attcgggtta gctttttcca gcacctgcgg gtcggtccag tcatcgcctt cgtcaacggt
    37621 atagatgatc ccgaacagtt catcgttggg taccgacccg ttgagcatct cgataacttc
    37681 ccgtcgcttg tcgtagcacg gcccctcaat gttgtacccg gcagtagtaa tggcccacat
    37741 cagtggctga cgtcgcgccc ccatcccggt aagcatcgtg gtgtaaagcg catcggtggc
    37801 gtgctcgtga tattcatcca ccaccgcaca gtggggtgat gaaccatcac cggggttacc
    37861 gatcagcggt tcaaaccgcg cgccatcctc cggacgatta atgtttgagg cgttaacctc
    37921 aatcccgaac gcttccgtca gcatgggtgt gcgtttacac atcagtcgcg ccgggcgaaa
    37981 gacttcccac gcctgtttct ctgtcgtggc accggaatac acttccgcgc caaactcgtt
    38041 atcacaggca aaacaataca gggcaacacc ggcagagatt gccgatttgc cgttcttacg
    38101 ggggatttcg gtatacacct cccggaagcg gcgcagccgg gaccctttat tgacccagcc
    38161 aaacgcacag cagaccacaa atagctgcca cggctccagc gtgatgggca tcctcttgaa
    38221 tgcccactca cccttggtgt gcggcaacag ctgaataaat ttggcggccc gttcagccag
    38281 gtccttgtcg aagcggtaac gaaacgactt acttttttcc gccatcaggt catcaagatg
    38341 gcgctggcag gcctgaatca caaactggca ggccacaatc tttccgcgca caacatcacg
    38401 ggcatactga ttggcagcat ttacgttggg gtaagatttc cggctcatga ctcgatgatt
    38461 ttcagaaacg ggttagtggc tttcttctgc cccgccaggc caatcagacg ctggcggctg
    38521 ctggggtcga gtccgagcat tgcccccgtg ctgctcatct cggactcctg ttcttttttg
    38581 gcggtcagct ccggattttt gaccctgccg cccattgcac cggtgatggt gttgccctgt
    38641 atggcaatat ttttcacggc acgtcgccag aactcatagg ccacgcacca ccgctcaagc
    38701 accgcgaggt cagtcacgca caacaggccc tgaccgcaga gttctttggt tgtcagttgc
    38761 cacatgatcg tggcgagagg gagattttct tctgcgaacc actccggtgg ctcaacacct
    38821 ttgatgggcg taaaaacagg ttcatcttta ttcagggctc gcttgccggg gtttccggcc
    38881 agcgccttgc gcgccgttgg cttggggcga cgcccggaac gccccgccgt tccagccata
    38941 tgcggcactc ctggttaaat ttcatttttc gcgggtataa aaaaacgatg gggcgggcag
    39001 tccggaagac gtcaggtcac agggatttga cccgcccctc ccctcagaca gttgagaatt
    39061 attatcactt taaccgttca cgggccgtct tcgccttatg acacggccag cacagactct
    39121 gcagattact gtcagcatca gtgccgccat gcgctttagg gatgatgtgg tcaacagttt
    39181 tcgcctcacg caccacacca gaacgcagac ataactgaca caggcctttg tcacgcttca
    39241 ggacacgcgc gcggatactg tcccacttcg aaccgtagcc gcgctgatga cgggattgtc
    39301 caggtttgta ttgcttccag ccttcgcttt tgtggctttc gcaatagcct gacgggtcag
    39361 tggtggtatt acggcaacct cgagcacggc aggctttcgg gattcgtggc ggcatatgta
    39421 ctaatctccg atttatccaa atttaactgc cataatgccg acattctctg ccattgttgg
    39481 ctccgtttat ccgttaaaag ggatatcagt taagttatcc cgtgtagggt ataaaccatt
    39541 atcaaagcca ctctgtaggg agtggctttt gtaatgacaa taaaaagccc cgcgaatacg
    39601 aggcttttag catgtggaaa aggaagttta ttgtaacttt tcaagataag gagctatgtt
    39661 gctaactttt ttgtagttca gatcatccgg tagttgaaca gcaggattat atacaaccca
    39721 tatagattca gacttgccat ctgaaccatc tgtgttttta catgagcgta acttgtaatg
    39781 aatgattcta tctacgtcag cgtactcgga ggttgcataa acttcatatt tattatctcc
    39841 atggaaaagg atagttttaa ctaattttgt agatgacatg tctgctcctt tgtaattgat
    39901 gggtatgaca tatgaccacc aattacaatg ccatatttct ggtgattatt gcagcagcga
    39961 aattacaaac caaacccaaa gatgggcttt gtaatgacta cagtaacgaa ctgcacaatg
    40021 cgcctgtatt tcgaggatga cgtccaaata cgttaatctt ctcgcgaacg ctctcactac
    40081 acattcgctc tacaattcgc caaacaacct tttcaggtaa aaatttcggc gctagtgctg
    40141 aaatagcacg ccacagaccc cgactaagcg agcatgctgt gctaccgaaa ctaaagatga
    40201 cgaacgaaat agacgtgata aacgcccagc acccagagag aaaagctgag atgcggtgat
    40261 aaagcttagt catgtattgc tcctgttttt ttggttttca tcgcccgatc atttcaggca
    40321 ttgcgtcctg atgtattcct gcaggtagtt aacctgcgcg gttatcctgt tgattccact
    40381 tctgagacgg taataattga gttcagcatc tgctgtaagt cttgggcttt ctccatcgcc
    40441 catgctgctg gctccggtcg ttgactttgc acaggtggcg gcgacttgca ggcgcttacg
    40501 accagcagaa acatcagcac ggagactttc gatagtcgcg ttagcatcag caagctcctt
    40561 tgtgtatctg gcgtcgagtt ctgctacatc acgttgacgc ttctgcatat cagcgatgat
    40621 ggatgcggct ttatcgcgct gctctttgta ggtcatggcg ttatcacggt aatgattaac
    40681 agcccatgac aggcagacga tgatgcagat aaccagagcg gagataatcg cggtgactct
    40741 gctcatacct caatctctct gaccgttccg cccgcttctt tgaattttgc aatcaggctg
    40801 tcagccttat gctcgaactg accataacca gcgcccggca gtgaagccca gatattgctg
    40861 caacggtcga ttgcctgacg aatatcgccg cggtcaatca tcggtaaagc gccacgctct
    40921 ttaatctgct gtaatgccac tgagtcctgg cttctgggag agaagtcttt cagcccaagc
    40981 tgcttgcggt aagcatccca ccaacgtgaa agaagttgat aacggcctgc agctgttgat
    41041 ttgagtttcg ggtttagcgt gacaagtttg cgagggtgat cggagtaatc agtgaagagt
    41101 tcgccaccga caataacgtc ataaccgtgg ttacgtgtcg gttgtcgccc gttatccgtt
    41161 ccttctgacc atgccaccat atccaggaaa gctttacgct gggaatttag tacctgcata
    41221 aattactcct tagagccacc aaacttgtta ccgattactc tcattgcagc cccacgaata
    41281 gcatcgacac cgatcaaccc aacgccacca ccaatggcaa cagaaagcga tttaggccat
    41341 ccgacatact caagcgcgga tgcaaaggtc agcgtcagag cgccacagag caaaatctcg
    41401 agcgtttttc tcttccagcc accaccaccg ccaaaatagg caatgcgcaa gccagccata
    41461 acgatcgaca taatcactgc gcccagcggt gtgtctccac gccaccagct ctggaccaac
    41521 tccagccaag tatttgggtt atgaggcatt tgtagttatc tctcacctcg ctgatacagc
    41581 aggtgcaaat tgaggaaaca tcatgtaccg caaatcagaa gcggaaacgt caaagaagcc
    41641 gagccaatgg ataactgcgg gatagactag gcccaacgaa tccccaggcc cagaaacgac
    41701 aaaacccgct cgacggcggg tttaagctgt gtggcgaagt aaccactctt aacacaatac
    41761 aatacttttt gcgtacgcgt tagtgttttg atagaatttt cagccacata aaaattcatt
    41821 cttataattc gggatatata atggatataa cttgtttaga atgtggcaat gttcttgacg
    41881 acccaactgt agcttgcgat aaatgcggtg ccacgcctca tgttgtagtg ctgggcaaac
    41941 aatcgtactt tcctattggt gctgtaacag caaatcttga aaaaaatgat tcaagagcat
    42001 ttgattatcg attaggtgaa gtttgggatc taaaaaatga agtcacatct gaattcataa
    42061 ccagaattga gaaaaaattt agccgaaaaa acaaatttca taacttccta gactcagatc
    42121 acaacccttc atccattcct acgatcctaa aaaaatacat taataaaaat aatgaattca
    42181 ttgatttatc tagagctata atagagaagc ttaaacataa tgctaataac gaatcgagag
    42241 ttgcccaact tcaggggggg agcgttgttt tcatccacta taagtctacc gaaccagagg
    42301 atttggggaa acttctaatc gtaatggttg ataaacaaag cgcctatgac tttgattcgg
    42361 ataagttgac cccaacaaga ttaaatccaa ttaataccga tgccttacgg caggctgcga
    42421 tgttcgattt aactttattc gaagccagtt atccagaaaa caagggtgac tcatatgtac
    42481 atttcttgca aggtaaatct aaaagcgatt ttttcaaaga ctcattaggt tgccgacatg
    42541 attcggacaa taaaagaagc attcagcaat tattcagtgc tatagatatt tttgctagta
    42601 aaaactcact cggtcgtgta ctgcgtgata ctattgacaa tgaagttaaa tccttactgg
    42661 aaaaaaaatc aaaagataa
//
LOCUS       DAZFXT010000027        42645 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.027, whole genome shotgun sequence.
ACCESSION   DAZFXT010000027 DAZFXT010000000
VERSION     DAZFXT010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42645
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.027"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            198..1418
                     /gene="ybdN"
                     /locus_tag="ACHKW0_001585"
     CDS             198..1418
                     /gene="ybdN"
                     /locus_tag="ACHKW0_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HFO7474440.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQFQPEWQCWEPDVEWVRQPPQDAITDPDFFCFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWCRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1391..2020
                     /gene="ybdM"
                     /locus_tag="ACHKW0_001586"
     CDS             1391..2020
                     /gene="ybdM"
                     /locus_tag="ACHKW0_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA];
                     GO_process: GO:0043170 - macromolecule metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043412 - macromolecule modification
                     [Evidence IEA];
                     GO_process: GO:0044238 - primary metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IbrB-like domain-containing protein"
                     /protein_id="HFO7474441.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(2021..3181)
                     /gene="ybdL"
                     /locus_tag="ACHKW0_001587"
     CDS             complement(2021..3181)
                     /gene="ybdL"
                     /locus_tag="ACHKW0_001587"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HFO7474442.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PHYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATIWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3290..4378
                     /gene="hcxA"
                     /locus_tag="ACHKW0_001588"
     CDS             3290..4378
                     /gene="hcxA"
                     /locus_tag="ACHKW0_001588"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HFO7474443.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNYLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4388..4585)
                     /gene="ybdD"
                     /locus_tag="ACHKW0_001589"
     CDS             complement(4388..4585)
                     /gene="ybdD"
                     /locus_tag="ACHKW0_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7474444.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4767..6872)
                     /gene="cstA"
                     /locus_tag="ACHKW0_001590"
     CDS             complement(4767..6872)
                     /gene="cstA"
                     /locus_tag="ACHKW0_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter CstA"
                     /protein_id="HFO7474445.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLIWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7053..7466)
                     /gene="entH"
                     /locus_tag="ACHKW0_001591"
     CDS             complement(7053..7466)
                     /gene="entH"
                     /locus_tag="ACHKW0_001591"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HFO7474446.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7469..8215)
                     /gene="entA"
                     /locus_tag="ACHKW0_001592"
     CDS             complement(7469..8215)
                     /gene="entA"
                     /locus_tag="ACHKW0_001592"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HFO7474447.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTAFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8215..9072)
                     /gene="entB"
                     /locus_tag="ACHKW0_001593"
     CDS             complement(8215..9072)
                     /gene="entB"
                     /locus_tag="ACHKW0_001593"
                     /EC_number="3.3.2.1"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HFO7474448.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9086..10696)
                     /gene="entE"
                     /locus_tag="ACHKW0_001594"
     CDS             complement(9086..10696)
                     /gene="entE"
                     /locus_tag="ACHKW0_001594"
                     /EC_number="2.5.1.-"
                     /EC_number="6.2.1.71"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HFO7474449.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTLVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCLLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10706..11881)
                     /gene="entC"
                     /locus_tag="ACHKW0_001595"
     CDS             complement(10706..11881)
                     /gene="entC"
                     /locus_tag="ACHKW0_001595"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HFO7474450.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSARRFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12256..13212
                     /gene="fepB"
                     /locus_tag="ACHKW0_001596"
     CDS             12256..13212
                     /gene="fepB"
                     /locus_tag="ACHKW0_001596"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HFO7474451.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(13216..14466)
                     /gene="entS"
                     /locus_tag="ACHKW0_001597"
     CDS             complement(13216..14466)
                     /gene="entS"
                     /locus_tag="ACHKW0_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042931 - enterobactin transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042930 - enterobactin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HFO7474452.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14577..15581
                     /gene="fepD"
                     /locus_tag="ACHKW0_001598"
     CDS             14577..15581
                     /gene="fepD"
                     /locus_tag="ACHKW0_001598"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HFO7474453.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15578..16570
                     /gene="fepG"
                     /locus_tag="ACHKW0_001599"
     CDS             15578..16570
                     /gene="fepG"
                     /locus_tag="ACHKW0_001599"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HFO7474454.1"
                     /translation="MIYVSRRLLITCLLLVSACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALSAMVGGIVTSLLVWLLSWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16567..17382
                     /gene="fepC"
                     /locus_tag="ACHKW0_001600"
     CDS             16567..17382
                     /gene="fepC"
                     /locus_tag="ACHKW0_001600"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7474455.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17379..18512)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKW0_001601"
     CDS             complement(17379..18512)
                     /gene="wzz(fepE)"
                     /locus_tag="ACHKW0_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator Wzz(fepE)"
                     /protein_id="HFO7474456.1"
                     /translation="MSSLNIKQGSDAHFPDYPLASPSNNEIDLLNLISVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISTLVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKAHVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGGVLLRYAMASRKQDAMMADHLV"
     gene            complement(18728..22609)
                     /gene="entF"
                     /locus_tag="ACHKW0_001602"
     CDS             complement(18728..22609)
                     /gene="entF"
                     /locus_tag="ACHKW0_001602"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HFO7474457.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPDVQAQTHTLATGPVNDLELALFPDVHGDLSI
                     EILANKQRYAEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADAHYQFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     ADVPDLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAPGRAP
                     KAGSETIIAAAFSSLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQVARQVTPGQVM
                     VASTVAKLATIIDAEEDSTRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPNGPMQTAANLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFEKIGPIIRATLNR"
     gene            complement(22606..22824)
                     /gene="ybdZ"
                     /locus_tag="ACHKW0_001603"
     CDS             complement(22606..22824)
                     /gene="ybdZ"
                     /locus_tag="ACHKW0_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HFO7474458.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22827..24029)
                     /gene="fes"
                     /locus_tag="ACHKW0_001604"
     CDS             complement(22827..24029)
                     /gene="fes"
                     /locus_tag="ACHKW0_001604"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0008849 - enterochelin esterase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HFO7474459.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTNVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PETPAKEIIWKSERLKNSRRVWIFTTGDATAGERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEQLKAGQVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24272..26512
                     /gene="fepA"
                     /locus_tag="ACHKW0_001605"
     CDS             24272..26512
                     /gene="fepA"
                     /locus_tag="ACHKW0_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HFO7474460.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYVTTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSKNKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26552..26650
                     /locus_tag="ACHKW0_001606"
                     /pseudo
     CDS             26552..26650
                     /locus_tag="ACHKW0_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518748.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            26687..27307
                     /gene="entD"
                     /locus_tag="ACHKW0_001607"
     CDS             26687..27307
                     /gene="entD"
                     /locus_tag="ACHKW0_001607"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009366 - enterobactin synthetase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009239 - enterobactin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HFO7474461.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27429..27581)
                     /gene="hokE"
                     /locus_tag="ACHKW0_001608"
     CDS             complement(27429..27581)
                     /gene="hokE"
                     /locus_tag="ACHKW0_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO7474462.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27932..28084)
                     /gene="hokE"
                     /locus_tag="ACHKW0_001609"
     CDS             complement(27932..28084)
                     /gene="hokE"
                     /locus_tag="ACHKW0_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HFO7474463.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            28053..28322
                     /locus_tag="ACHKW0_001610"
     CDS             28053..28322
                     /locus_tag="ACHKW0_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474464.1"
                     /translation="MTAARAYFVSMFILPPCEEGTTIQIVQVWVGSPRVDRNIVLGLH
                     LSRPQSKPETGKLQAPAVILPLLSKIAVYLYSKSCFGLLTPHLIN"
     gene            28526..29644
                     /gene="ybdK"
                     /locus_tag="ACHKW0_001611"
     CDS             28526..29644
                     /gene="ybdK"
                     /locus_tag="ACHKW0_001611"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbdK family carboxylate-amine ligase"
                     /protein_id="HFO7474465.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            29710..29958
                     /gene="ybdJ"
                     /locus_tag="ACHKW0_001612"
     CDS             29710..29958
                     /gene="ybdJ"
                     /locus_tag="ACHKW0_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HFO7474466.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            30023..30391
                     /gene="ybdF"
                     /locus_tag="ACHKW0_001613"
     CDS             30023..30391
                     /gene="ybdF"
                     /locus_tag="ACHKW0_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7474467.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALIRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            30485..31138
                     /gene="nfsB"
                     /locus_tag="ACHKW0_001614"
     CDS             30485..31138
                     /gene="nfsB"
                     /locus_tag="ACHKW0_001614"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HFO7474468.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            31246..32493
                     /gene="ybdG"
                     /locus_tag="ACHKW0_001615"
     CDS             31246..32493
                     /gene="ybdG"
                     /locus_tag="ACHKW0_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HFO7474469.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(32561..33937)
                     /gene="pheP"
                     /locus_tag="ACHKW0_001616"
     CDS             complement(32561..33937)
                     /gene="pheP"
                     /locus_tag="ACHKW0_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HFO7474470.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(34039..37182)
                     /gene="cusA"
                     /locus_tag="ACHKW0_001617"
     CDS             complement(34039..37182)
                     /gene="cusA"
                     /locus_tag="ACHKW0_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HFO7474471.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAVPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            complement(37194..38417)
                     /gene="cusB"
                     /locus_tag="ACHKW0_001618"
     CDS             complement(37194..38417)
                     /gene="cusB"
                     /locus_tag="ACHKW0_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HFO7474472.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            complement(38433..38765)
                     /gene="cusF"
                     /locus_tag="ACHKW0_001619"
     CDS             complement(38433..38765)
                     /gene="cusF"
                     /locus_tag="ACHKW0_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HFO7474473.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(38789..40171)
                     /gene="cusC"
                     /locus_tag="ACHKW0_001620"
     CDS             complement(38789..40171)
                     /gene="cusC"
                     /locus_tag="ACHKW0_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HFO7474474.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            40328..41011
                     /gene="cusR"
                     /locus_tag="ACHKW0_001621"
     CDS             40328..41011
                     /gene="cusR"
                     /locus_tag="ACHKW0_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HFO7474475.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            41001..42449
                     /gene="cusS"
                     /locus_tag="ACHKW0_001622"
     CDS             41001..42449
                     /gene="cusS"
                     /locus_tag="ACHKW0_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HFO7474476.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLSVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASIISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDECQVAGDPLMLRRALSNLLSNALRYTP
                     PGEAIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT        10181 a        11784 c        10906 g         9774 t
ORIGIN      
        1 aataatttgg tgttctcccg atttattgac cgtctaaatg agagtttttg atatataact
       61 acagacagct actataactt tcatctattt attcacagcg cgaatgaaaa ataaaacact
      121 ctgctttgca gtgtttactt tataattaac agacttatct cactgatcac cctgtaacgt
      181 tcagagagcg tcttcagatg tctatttata aaattcctct tccgctcaat attctggaag
      241 ctgcacgaga gcgtattacc tggacactca acaccctacc ccgcgtttgt gtctcatttt
      301 ctggcggcaa agactctggt ttaatgctgc atctgacagc cgaacttgcc cgacaaatgg
      361 gcaaaaaaat ctgcgttttg tttatcgact gggaggcgca attctcttgc accattaact
      421 atgttcagtc cctgcgcgag ttgtacaccg atgtcatcga agagttttac tgggttgcgc
      481 tcccgcttac gacgcaaaat tccctttcac aattccaacc cgaatggcag tgctgggaac
      541 ctgatgtcga atgggtgcgt cagcccccgc aagatgcgat aaccgatcct gactttttct
      601 gcttttacca gcctggcatg accttcgaac aatttgtacg tgagtttgcc gaatggtttt
      661 cacaaaaacg tccggcggcg atgatgatcg gcatccgtgc ggatgagtcc tacaaccgtt
      721 ttgtcgccat cgccagttta aataaacaac gttttgccga cgataaacca tggactacgg
      781 ccgcaccagg cggtcatagc tggtacattt accccattta cgactggaaa gtggctgata
      841 tctggacctg gtatgcaaat catcaaagtc tctgcaaccc actgtataac ctaatgtatc
      901 aggcaggcgt tcctctgcgt catatgcgaa tttgcgaacc ttttggcccg gagcaacgac
      961 aaggattatg gctctatcac gttatcgaac cggatcgctg ggcagctatg tgcgcacgag
     1021 tcagcggggt aaaaagtggc ggcatttacg ccggacatga caatcatttc tatgggcatc
     1081 ggaaaatcct caagccagaa catttagact ggcaagaata tgcattattg ctgctcaata
     1141 gcatgccgga aaaaacagct gagcattacc gcaataaaat cgccatttat ttgcactggt
     1201 atcagaaaaa aggcatcgag gttccacaaa cccagcaagg ggacattggc gcgaaagata
     1261 tcccctcctg gcggcggata tgcaaagttt tactcaataa cgattactgg tgtcgagcat
     1321 tatcatttag tcctacgaaa gcgaagaact atcagcgtta taacgaacgg ataaaaggaa
     1381 aacgtcagga atgggggata ctatgcaaca acgattaacg caggatttaa cgcaatttct
     1441 cgccagcttg ccggaagatg atcgcatcaa agcgattaat gaaatccgca tggcgatcca
     1501 tcaggttagc ccctttcgtg aagagccagt ggattgcgtt ctgtgggtta aaaacagcca
     1561 acttatgcct aatgattaca acccaaacaa cgtggcacca cccgaaaaaa aactgctgaa
     1621 aaaatccatt gaaatagatg gttttaccca acccattgtg gtcacgcata cggataaaaa
     1681 tgccctggaa atcgtggatg gttttcaccg ccatgaaatt ggtaaaggct catcatcatt
     1741 aaaattacgc ttaaaaggtt atttgccggt gacctgtctg gaaggtacgc gtaatcaacg
     1801 catcgccgcg actattcgtc ataatcgcgc tcgtggacgc catcaaatca ccgccatgtc
     1861 ggaaatcgtc cgtgaactta gccagttggg atgggacgat aataaaattg gcaaagagct
     1921 gggaatggac agcgacgaag tgctgcgcct gaagcaaatt aacggcctgc aagagttgtt
     1981 tgccgaccgt caatattccc gcgcctggac ggttaaatag ctaaagctgg cgcaggcgtt
     2041 cagctgctgc cagcaacgtc gattccttct tggcaaaaca gagacgaatc agtttatgtg
     2101 ggaagggatc ggcgcaaaac accgacagcg gaatcgccgc tacgccgtgc tcctgcgtca
     2161 gccactggca aaactcaaca tcatccaggg tagaaaccgc gctgtaatcc accagcaaaa
     2221 agtatgtacc ttcacacggt aaaatctcca gccggctttc atttaaagca ttcaccagaa
     2281 tatcgcgctt ctggcgataa aagtccggta acgcaagata atgctcaggt tctgcacgta
     2341 gcatatcagc aagcgccagc tgtgccgggg tattcaccga aaaggtcaga tactgatgta
     2401 ccttgcgaat ttcggcgctg atgggcgctg gcgcaacaca ataacccact ttccagccgg
     2461 tcatatgata ggtcttgcca aatgaagaaa ccgccactgc ccgctcacgc agctgcggat
     2521 gcgccagcac actggcatgg ccctgttgtg aaaagttgat gtgctcgtag acttcatcgc
     2581 taatgacaaa aatctcgtgc ccggcgatcg cctgccacaa agcggcgaaa tcagcctgct
     2641 gccagatagt tgcactgggg ttatgcggag tgttgaggat caccagtctg gtgcgctcgc
     2701 ttaataatgc ggcaaattcc tgccagtcaa cgcgaaaatg cggtggttgc agtgccatac
     2761 gcttcactat tcccccagaa agcgcgatgg cgggggagta actgtcatag ctgggatcaa
     2821 aacaaatcac ttcatcgcca ttgcgcacca gtgcggtaat cgccgcgtat aacgcttccg
     2881 tcgcccctgc cgttacggtg atatcgctat cggcatctgg ttgatagcca tacaaacgtt
     2941 ccgttttctg agcaatcgcc tcgcgcaagg cctgcacgcc ggtcatgggc gcgtattggt
     3001 ttgccccctg ggcaacgtgg tacgccagcc gctcctgtaa atagtgcgga ccatcaaaat
     3061 caggaaagcc ttgcgacagg ttaatcgcct ggtgttgctg cgccagcgcg ctcatctggg
     3121 tgaaaatagt ggtgccaagt tgtggaagtt tgctttgtgg aatcagaggg ttatttgtca
     3181 tttttattgt acctgacgaa gttgcgtgtt gaaggcacta taacacgatg ctagtatttg
     3241 gcaatcaaga cgtttagatg tctaaataaa acaataagga caacacaaca tgcctcacaa
     3301 tcctatccgc gtggtcgtcg gcccggctaa ctacttttca catccaggaa gtttcaatta
     3361 cctgaacgat tttttcactg atgaacaact ttctcgcgcg gtgtggatct acggcaaacg
     3421 cgccattgct gcggcgcaaa ccaaacttcc gccagcgttt ggacttccag gggcaaagca
     3481 tattttgttt cgtggtcatt gcagcgaaag cgatgtacag caactggcgg ctgagtccgg
     3541 tgacgatcgc agcgtggtga ttggcgtcgg cggcggcgca ctgctcgaca ccgcgaaagc
     3601 cctcgcccgc cgtctcggtc taccatttgt tgccgctccg acgatcgccg ccacctgcgc
     3661 agcctggaca ccgctctccg tctggtataa cgatgccgga caggcgctgc attatgagat
     3721 tttcgacgac gccaatttta tggtgctggt ggaaccggag attatcctca atgcaccgca
     3781 acaatatctg ctggcgggga tcggtgacac gctggcgaaa tggtatgaag cggtggtgct
     3841 ggctccgcaa ccagaaacgt tgccgctaac cgtgcggctg gggatcaata atgcgcaggc
     3901 cattcgcgac gtcttgttaa acagtagcga acaggcactt gccgatcagc aaaatcagca
     3961 gttaacgcaa tcattttgcg atgtggtgga tgcgattatc gccgggggtg gaatggtcgg
     4021 tggtctgggc gatcgtttta cgcgtgtggc tgcggcccat gccgtgcata acggtctgac
     4081 tgtcctgccg caaaccgaga aatttctcca cggcaccaaa gtcgcttacg gaattctggt
     4141 gcaaagcgcc ttgctgggtc aggatgatgt gctggcgcaa ttaaccgggg cgtatcagcg
     4201 ttttcatctg ccgactacgc tggcggagct ggaagtggat atcaataatc aggcggagat
     4261 cgacaaagtg attgcccaca ccttgcgccc ggtggagtcc attcattacc tgccagtcac
     4321 gctgacacca gatacgttgc gtgcagcgtt cgaaaaagtg gaatcgttta aagcctgacg
     4381 tgcaaattta gcagcagcgc gcgccgcctt ttccaccgta gcgcgcgtcc tgccgctcac
     4441 ggaaaaactc ctcgtaggtc atcggcgttt gatcgggatg gttaacccgc atatgttcga
     4501 catagttgtc gtaatcaggc ataccaatca tcagcttcgc cgcctgacct aaatattttc
     4561 cggcttttgc cagtgaatca aacatcttgg cctccgctaa ccatgttgct gaaagagtgc
     4621 cggatgcggc gtgaacgctt tatccggcct acaaatgcta tcaacgcact cggtcagtcc
     4681 cctcgcccca tcggggagag ggttagggtg agggggaacc ctcggcacta tgccaacatt
     4741 cgccaacatc ccccctcact ctgactttag tgcgcacctt tcgcctgcgc tacgatctcc
     4801 tcaacatttt ccggcattgg ctcatacggc gtttctttcg ccgttggctt cggctctttc
     4861 aatgccgcaa gtgccgtctt aatcgagaac agtgccagaa ccaccacgac caccataaag
     4921 aagatggtta acccggcatc cagacggttg ttgaacacca gttgcgccag ctgtgactca
     4981 gtatactgcg acggaatatt gccgctgtcg atcattgcct ggaacttgtt agcaatggcc
     5041 aggaagccga ctttcgcatc cgggctaaac gctttctgcc agcctgcggt cagggtacaa
     5101 atcagtagcc aggccgttgg taccagcgcc acccaggcgt aacgttgacg tttcatcttg
     5161 aacaacacca cggcacagag catcagcgcc atccctgcca gcatctggtt ggcaatacca
     5221 aacagcggcc acagagtgtt aataccgccc agcggatcga ccacgccctg atggaggaag
     5281 tagccccacg ccagtacgca cagcgccgtt gccagcaggt tagcaggcag tgaatcggtc
     5341 cgcttcaggc caggagacac cacgcccagc agatcctgca acataaagcg cgcagcacgc
     5401 gtacccgcat ctaccgccgt cagaataaac agagcttcaa acagaatggc gaagtgatac
     5461 cagaacgcca catccatcat accacccagc gcaccgtgca ggatgtaagc catccccacc
     5521 gccaacgtcg gcgcaccgcc cgcacgggaa atgatcgact gttcacccac ttcgctggca
     5581 atctggttga gcgtatctgg tgtaatgcta aagccccagc tactcaccac ctgcgcggca
     5641 gaagcgacca catccgccgt ccctgccgga gccagcaccg ccattgggct gttcatggca
     5701 aagtaaacgc ccggatcgat gatacaggcg gaaaccagcg ccataatcgc cacgaaggat
     5761 tccatcaaca ttccaccgta gccgataaaa caagcctgcc cttcgttcgc cagcatcttc
     5821 ggcgtggtgc cggaagagat cagcgcatgg aagccagaca ccgcgccaca ggcgatggtg
     5881 ataaacagga acgggaacag gttaccggtc cataccgggc cagtgccatc gacaaatttg
     5941 gtcagcgcag gcatggtcag cgtcgggcgc ataatcaaaa tgcctaccgc cagaccgacg
     6001 atcgtcccga ttttcaggaa ggtagagagg tagtcacgcg gggccagcag caaccacacc
     6061 ggcagcaccg ccgccacaaa accgtaaccc accagcatcc agatcagctg cacgccggta
     6121 aagtcaaagt acggtgccca ggtcggactt tctgccaccc agccgccaga gataatggcg
     6181 aaaatcagga ataccagacc gatgaccgac acttcaccaa tacgccccgg acgcagatag
     6241 cgcaggtaga tccccataaa cagcgccagc ggaatggtga acgcaacggt atacgttccc
     6301 cacgggctat gagtcagggc tttcaccacg atcatcgcca gtactgcaag gataatgacc
     6361 atgatcataa agcaggccac cagcgctatc accccggcgg ttggccccat ctcttctttg
     6421 accagctcac ccagcgatcg accgtcacgg cgcgtagaaa caaacagcac catgaaatcc
     6481 tgcaccgcac cggcgagaac caccccggcg agcagccaga tcatccctgg caggtaaccc
     6541 atttgcgccg ccagtaccgg cccaaccaac ggacctgctc cggcaatggc cgcaaaatgg
     6601 tgaccgaaca gcactttctt gtccgtcggc acatagtcca gcccgtcgtt atggcgcacc
     6661 gctggcgtca tacgcgtcgg gtcaaccgcc agcacatttt tggcgatata cagcccataa
     6721 aaacggtaag cgatcagata gatacagacc gacgccacca caatccacag cgcgttgatc
     6781 tgttccccac gatttaaagc aatgtatccc agagcaaatg ctcccattac agagagcact
     6841 gtccagacga ggtatttccc tgatttgttc atagttgtta tccgtgtgcg tgtccataga
     6901 tatgttacat tttgtttcta taacatcttt gtacgattta acaacacggt aaccatgtaa
     6961 atcggagttg aaaccaatat ttaacttaac aatgttaact cgatcactcc gttaaccgag
     7021 ttttcctgca atctcattta attctgtcgg gctcatccca aaactgccgt acccagccga
     7081 caagtgcagc aacgccgccc ctgttcatcg aaaacgacga tttcccagct ctgattttgc
     7141 cgcccaagat gcagcggctg gcagacgccg cgtaccttgc cttcagacac cgggcgatga
     7201 tgggtggcgt taagttccgt acccaccaca cactgcccgt cacgggtcat cataaatccg
     7261 gccatcgatc ccagcgtttc agccagcgcc gctgacgcgc cgccatgcag caggccaaac
     7321 ggctgatgag tacgggtatc aaccggcatt tcggcttcca gcacatcatc tcccagacgg
     7381 gtatacacaa ttcccagatg cgccaccatt gtgttatcgc tggtggcgtt cagttcgtcg
     7441 agcgttaaat ggcgtttcca gatcatgctt atgcccccag cgttgagccg ccatcgacca
     7501 caatatcctg tagggtgata tggctggcga ggtcagaggc gaggaacaaa atcgtgttgg
     7561 cgatctcttg tggacgggcg attttcccca gcggaatgcc gagtttaaac tgctcgccaa
     7621 agccgcgaat acgctgttct tcggcgtcat cgctcaccca cagcgtgcgt tgcatatcgg
     7681 tgtcggtgga gccaggcgaa accacattac agcgcacgcc gctacccgcc agttccagcc
     7741 cgacgctcaa cgccaggctt ttcagcgccg ctttcgatgc gccataagca ctcatgccaa
     7801 tacgcggcgt gtgcgcggcg tcggacgcca cagtgacaat cgccccgccc cgctgacggc
     7861 gaaactggtt catggtttgc tggaacaggt taaacgcacc gccgacgtta accgcaaaag
     7921 tctgctgcca gtcctcttta ctgagctgat cggtcgcgcc catgcgcaaa attcccgccg
     7981 cattgaccag cacgtccagt cgctccgttt cagctaacag tcgctgacat acctgcgcga
     8041 cctgcgcagc atcggcaaca tccatcactt cggtcgcaaa gggatattgc tcctgagcga
     8101 acgcttgatc aaaagcggta actttcgctc ccgcctcaac aaacgccagc gccgtggcgt
     8161 agccgatacc tttacctgcg ccggttaccc agacattttt accgctgaaa tccattattt
     8221 cacctcgcgg gagagtagct tccaccaggc gtcgatggtc gggtttttcg ccagcatgac
     8281 aaagtcgata tcaccatgca ctttgcgcca gcgcgccgcc agcgccatca tgcgcaccga
     8341 atccagaccg tagtcgatca ggttgtcgtc atcgaacggt tcatcggact cgtccagcaa
     8401 cggcaggata acctcacgca gcgccgcttt gctggcgggg acaggtgctg gcagtaattc
     8461 ttcagtcatc accacccggc cagaccgccc ggccacatat ttcagcgaca tcaaatgctc
     8521 gtcacggctg aaatcggcca gcgcatccgc caccataaac ggtttaatat cgcgcataaa
     8581 tgcgtcggtt gcggtggtca tacagccaat gtgggcatat accccggtaa taatcagctg
     8641 gttacgtcca ctctctttga gcatttgctc cagcggagaa cgatgaaacg cgctgtagcg
     8701 ccacttcacc agcaccgtgt cgtcggcatc tggcgtcagg cgatccacca ccttttgctg
     8761 ttccggcgaa cgggtcaggc ccggccccca catatcattc aacagcgccc gatcttcatc
     8821 gctctgctct ttcggctggg cggtgtaata aaccggaata ttgtgctgtt tgcagtagtc
     8881 gcgcagcgca gcaatattcg cgatcacctg ctccatcatc gggcagttct cgccccagaa
     8941 gctgacaaaa tagtcctgca tatcatggat taacaacgcg gcacgttgcg gttcaaaggc
     9001 ccagtcaact ttattcttag ggatatcgtg agactccggc agtgcgtaag cctgtaattt
     9061 tggaatagcc atcgtgttct ctccttcagg ctgatgcgcg tgacgccagc cactgacgta
     9121 attgtttttt atcgactttc ccgaccgccg tcagcggaag tgaatctaca cactccacgc
     9181 gatccggtaa tttaaattcg gcaataccct gttcacgcag gaaacgacgc acctgcaccg
     9241 cgcgcagcgg ctcttttacc accagataag cgcagctttt ttcgcccatc agctcatctt
     9301 ccatgctcac cagtgcggcg tagatcaccg ccgggtggcg cagcagcagg ttttcgatct
     9361 cttcggcagc gatcttctca ccgccacggt tgatctgatc tttctcgcgc ccctgcacgg
     9421 tgatgtaccc ctctggatca atagagatca gatcgccgga acagtaaaaa ccgttggcat
     9481 caaaggcact ggcattgtgc tgcggacttt tgtaataacc gcggaaggtg tacggcccgc
     9541 gcgtcatcag gcgtccaact tccccttgcg gcagtggatt tccttcagca tcagcaaccc
     9601 atacttcatc gtccggacac attgggtaac cctgggtatg gataattttc tccgcgctat
     9661 catcaagtcg ggtgtagttc accagccctt ccgccatgcc aaacacctgc tgcaacaggc
     9721 agccaatctc agcgggaata cgcgccgcaa gggtggctga aagacgtgcg ccgccgacct
     9781 gtaacagttt cagcgaggca agctgcgccc ggctttcacc ttcggtcagc gcctgcaacc
     9841 acaggctgac tgccggtggc accagcgcgg tgacgttcac ctgatgtttt tcaatcaatg
     9901 ggaagcaaag cgtggcgctg ggatcggccg ccagaacaag cgttcctccg gcaagaaaga
     9961 cgcccagcga tcccggcgaa ctcatggcgt agttatgagc cgccgggatc gcgcacaggt
    10021 agcgtgtctg ttgtgtgaac tgacaaatct cgacgctacg acgcacgctg tagtagtagt
    10081 cgttatgagt gcgcgggatc agtttcggtg tgccggtggt gccgccggaa agctggaaat
    10141 aggccacttc atcagcaggt gatggcgtgg cagtaaaatc ctcagccgga tggttaatcg
    10201 catcctgcaa gttatgctca ccgctgtcgt tgtgcagttg caccacgcga atggaggaat
    10261 gttctgtgac gaaagtattg aggaaatcat ccccgctaaa cagcgcatgt tggcgatcgg
    10321 caatcagcaa tgcgggttca atctggctgg cataggcgtt cagttcacta cgctgatggc
    10381 tgaacagcgc cagcaccggc gcaacgccca gtttcagcag cgcgaaaaag gtaatataca
    10441 attcagcgac gttacccagt tgtaccagcg cggtttcacc aggtttaatg ccctgacggc
    10501 gtaaactaca cgcgaggtta tccgccgcct gattcagctc ccggtaactc aactgtcgct
    10561 cgccgtcgat aaccgcgatg ctgtcactcg cagcgtggcg agtcagaatg tcggtcagcg
    10621 gcaaatcctg ccagtagcct ttttcccgat agcgacgggc aaactcttcc ggccagcggg
    10681 tgaatggaat gctcatcctc gctccttaat gcaatccaaa aacgttcaac atggtagaaa
    10741 gtttgacgcc tgtttcgcgc cactcaccca acggtgacga cgcaggcaca atccccgctc
    10801 cggcaaacag acgcacctga ttttcccgca gcttcgcgca gcggatggtc accacccatt
    10861 cgccgttacc ttcgctgtca caccaaccca caatgccgcc aaacagttcg cggtcgaatg
    10921 gctccagttc agcaataacc tgggtcgcgg cctgatgcgg gaaaccgctc agcgcggggg
    10981 ttggatgcag cagacaggcc agagtcagtg cgttttcttg cgaattcgct ttaccttcaa
    11041 agggagttgc gagatgccac agcgtcggcg tggtaatcaa ttgtggagag gagggaacgt
    11101 gtaactcact actgcgttcg cgcagtacct ctttcatcgc ctgagtcacc agttcatgtt
    11161 catggcgatc tttttctgac gccagcagac gattacccgc ttcgcgatcg agcacttcat
    11221 ccggctgacg acgcgcggaa ccggctaacg gaatggagct aaaacgctcg ccgtctttac
    11281 gtagcagcag ttccgggctg gcccccagca ggacgccacc atcagccagc gggacatgga
    11341 agttgtaact aaccgggttt tgcgcaatca accgttccag caatacgcca ctatcaatgg
    11401 cggcgtcagt ggtgatatca atcaatcgtg acaacaccac tttgtcgacc tgcggcgtgg
    11461 cggtaagtgc ggcggcgcgg gcaaccatct gttcaaacgt ggtttgttca ggaattgcct
    11521 ggcgttccac cacgttcagc gactggctgc gggtaaaacg gcgggctgag gtctgttttt
    11581 cctggcggga gaacgactgc caggattcgg gaatatacag cgacgaaggc tgacgtggat
    11641 cgaagggaat cgccccgacc atcaccggat ttttgatgcc ctgcgctttg gcatcggcaa
    11701 acagcgcggc gagtttttgc tggaagggac tgtcgggcga atccccgttc acagccggtt
    11761 catcgaagcg ggcgaaacat cctgacgtcg taaaactgcg gtacggcgac ataaagaaaa
    11821 agcgattggg cgcaagtgtt gccatggtct gctgtacttc ctcagccagt gacgtatcca
    11881 tatcatcctc cacaaaatga taaaggcttt aataatgatt atcatttata ttttcggtcg
    11941 ctaacctaaa agcaaacgcg cactatgtca actcttgagg taacgcaatc tgcaactcgg
    12001 ggttgcatct ggttgtggtt acaatgaaaa tgagaagcat tattgatgga ttcgcataag
    12061 cgcaatgtga tgtcctgcgc cgttctgccc cctctccctt ccagggtgag ggctggggtg
    12121 agggttaatg ttcgcaccag tgctggctgt tcccctcacc ctaaccctct ccccaaaggg
    12181 gcgaggggac ggattgtgcg ctttgtcgaa tttgtcatta cgcccttaac cttattaata
    12241 acaggaagct gatttgtgag actcgccccg ctctaccgca acgcccttct attaacagga
    12301 cttttgcttt caggaatagc cgcagttcag gccgctgact ggccgcgtca gattactgac
    12361 agccgtggca cacatacact ggaaagccag ccgcagcgta ttgtttccac cagcgtcacc
    12421 ctgaccggct cactgctggc gattgatgct ccggtgatcg ccagcggcgc gaccacgccg
    12481 aataaccgcg tcgcggatga ccagggcttt ttacgccagt ggagcaaggt ggcgaaagaa
    12541 cgcaaactgc aacggctcta tatcggcgaa ccgagcgccg aagccgttgc cgcgcaaatg
    12601 ccggatctga ttttaattag cgcaaccggc ggggattcgg cgctggcact gtatgatcag
    12661 ctttccacca tcgccccgac attaatcatc aattacgacg acaaaagctg gcagtcgctg
    12721 ttaacgcagc ttggcgaaat taccgggcat gagaaacaag cggcagagcg gattgcacag
    12781 tttgataagc aactggcagc ggcgaaagag caaatcaaat taccgccgca gccggtcact
    12841 gccattgtct ataccgccgc tgcacacagt gccaatctct ggacgccgga atcagcacaa
    12901 gggcagatgc tggaacaact cggctttacg ctggcgaagt tgcccgcagg gttaaacgcc
    12961 agccaaagcc agggcaaacg ccatgacatc attcagcttg gtggggaaaa tctggctgct
    13021 gggttaaatg gcgagtcact attcctgttc gccggtgatc agaaagatgc cgatgcgatt
    13081 tatgccaatc cactgctcgc gcaccttccc gcagtacaaa acaagcaggt ttatgcgctg
    13141 ggaaccgaga cgttccgtct ggattactac agcgccatgc aagtgctgga acgacttaag
    13201 gcgctgtttt aagcattaac tgtcagacgc tgtcacctgc ggcggcgtct ggcgaaaacg
    13261 tcgcaactcc accagcacca gcaataacaa cacgccgata atcaacaaac caaaaccgct
    13321 cgcgcttgcg gaagcaaccg gtgtcatcat cgcgcccaaa ccacccagca gcgccgcgcc
    13381 tatggcatcg cccgtcacgt tctgcgccgt ccacaaaccg ttaatccgcc ctaacatcgc
    13441 ttccggggtt tgcgtttgca gcattgtata ttgcagcaac gaactaaccg cgctcagcca
    13501 gccgaacagc gccagacaaa ccacgcctaa aatccacatc ggcatcaggc caaacagacc
    13561 aatggcgagg aacgatccca gcgtggagag cagcatcaat aaccctggtc gcgcactatg
    13621 tgccagcttc ccgctggtta acgcaccaat agccgcgccg agcgggatcg ccgcgtagag
    13681 aaaaccaatc tgtgccgctg acatctgcca gttgtcagcc agcgccggat acagtacccg
    13741 caccgcgctc gccatcgtca ataaaccacc cagcagcgca atcccgccca ccagcgggct
    13801 ggcaagcaga aaacgaaatc ctgccagtaa tgatttcaac ggatgctcac gcggctgcgg
    13861 tggcggtggc aacgccggaa ggcttaacaa cggtagcaag gtaataaacg tgcccgccgc
    13921 cgccagcccg tagttccagg ctacgccacc ggtcgccagc aataaaccgc caatcatggg
    13981 cgaaatcacc gaccccagac gcacggtcaa catggtgatc gccccggcct gcattaagtt
    14041 ttcacgccct accagtgcag gtgtcgccgc cagtagcgcc gtaacgccaa gtgatgcgaa
    14101 aaaaccatcc cataaaccaa gtaaatagat tgccagcaat gacggttcag gcagcagtgc
    14161 attaaggcac agtccaatga agccaatgcc acaggtgccg cgcgccagca aaatcacttt
    14221 tttgcgttca tagcgatccg ccagcacacc gccgaccatc aggccaacaa acatcgcgcc
    14281 gccggtcagc gtcaccgaaa gccccacctg ccaggtagaa tgtgtcatca tctggatctg
    14341 caccggcacc gcgacgccga gcaaacccag agaaacaata gagatgaaac gagcgaggaa
    14401 tactgcgcga aacgccgggt gcgttttcaa caggctgagg ttaagcagcc aggattgttt
    14461 attcattaca atgccttgcc atcaaatatt ttaaatacct attcttaggc tgcacatgct
    14521 aacatatcca aataagatcg ataacgataa ttaatttcat tatcatggaa gttcgtatgt
    14581 ctggttctgt tgccgtgaca cgcgccattg ccgtgcccgg attgctgtta ttactgatta
    14641 tcgcgacggc attaagtctg ctcattgggg caaaatcact ccccgcttcc gtagtgctgg
    14701 aggccttctc cggcacctgc cagagcgccg actgcaccat cgtgctcgac gcgcgtctgc
    14761 cgcgtaccct tgccggttta ctggcaggcg gcgcgcttgg ccttgccggg gcgttaatgc
    14821 aaaccctcac ccgtaaccca cttgccgacc ccggcttgct tggcgtgaac gccggagcca
    14881 gctttgccat tgtgctgggt gcggcgctgt ttggttactc ttccgcacag gaacaactgg
    14941 cgatggcctt cgccggggcg ctggtggcct cgttgattgt tgcctttacc ggcagccagg
    15001 gcggcgggca gttaagtccg gtgcgtttaa ccctggcggg cgtggcgctg gcggcggtgc
    15061 tggaaggact gaccagcggc atcgccctgc ttaatcctga cgtctacgat caactgcgtt
    15121 tctggcaagc cggttcgctg gatattcgca atctacatac cttaaaagtg gtgctgatcc
    15181 cggtgctgat cgccggagca actgcgctat tactgagtcg cgcgctgaac agtttgagcc
    15241 tcggcagcga caccgcgacg gcgctgggca gtcgcgtggc gcgcacacag ttgattggtc
    15301 tgctggcgat taccgtgctt tgtggtagtg cgacggcagt agttggcccg attgccttta
    15361 ttggcctgat gatgccgcat atggcgcgtt ggctggtggg tgccgatcat cgctggtcgc
    15421 tgcccgtcac gctacttgct acccctgccc tgctgctgtt tgccgatatc atcgggcggg
    15481 tgattgtgcc cggcgaactg cgcgtttctg tggtcagtgc gtttattggt gcaccggtgc
    15541 tgatcttcct cgtgcgacgt aaaacgcgag gtggtgcatg atttacgtct ctcgccgatt
    15601 actcatcacc tgtttgctgc tggtttccgc ctgtgtggtt gcaggtatct ggggattacg
    15661 cagcggtgcc gtcacgctgg aaacctcgca ggtattcgcc gcgctgatgg gcgatgcgcc
    15721 gcgcagtatg acgatggtgg tcaccgaatg gcgtttacca cgcgtgctga tggcgctgtt
    15781 gattggcgca gcactgggcg tcagtggcgc gatttttcag tcgctgatgc gtaacccgct
    15841 cggcagccct gacgtaatgg gctttaacac cggggcgtgg agcggcgtgc tggtggcgat
    15901 ggtgctgttt ggtcaggacc tgacggctat cgcgctgtca gcaatggtgg gcggcattgt
    15961 cacttcgctg ctggtctggc tgctctcctg gcgcaacggc atcgacacct ttcggttgat
    16021 tattatcggt atcggcgttc gcgccatgct ggtggccttt aatacctggc tgttgctgaa
    16081 agcgtcttta gaaacggcgc taacagcagg tttgtggaat gccggatcgc tcaacggcct
    16141 gacgtgggca aaaacctcgc cttccgcacc catcattata ttgatgctca ttgccgccgc
    16201 cttactggta cgccggatgc gcttgctgga aatgggcgat gataccgcgt gtgcgctggg
    16261 cgtcagcgtc gaacgttcgc gtctgttaat gatgctggtt gcagtggtgc ttaccgctgc
    16321 ggcaacagcg cttgccgggc cgatttcctt tattgcttta gtcgcaccgc acattgcccg
    16381 acgcattagc ggcaccgctc gctgggggct aacccaggcg gcgctatgcg gggcgctgtt
    16441 actgctggcg gccgatctct gcgcccaaca actgtttatg ccgtatcaac ttccggttgg
    16501 cgtcgttacc gtcagcctcg gcggtattta ccttatcgtc ttgttaattc aggagtctcg
    16561 caaaaaatga ccgaatcagt agcccgtttg cgcggcgaac agttaaccct cggatatggc
    16621 aaatataccg ttgcggaaaa tctgactgta gaaatacctg atggtcactt cacggcaatt
    16681 atcgggccaa atggctgcgg taaatccacg ttactgcgta ccttaagccg cctgatgacg
    16741 cctgctcatg ggcatgtctg gctggatggc gagcacattc aacattacgc cagtaaagag
    16801 gttgcacgcc ggattggtct gttggcgcaa aatgctacca cgccgggcga tatcaccgtg
    16861 caggagctgg tggcgcgtgg acgttatccg catcaaccgc tgtttacccg ctggcgcaaa
    16921 gaggatgaag aagcggtaac gaaagcaatg caggccacgg gaataactca tctggcagat
    16981 caaagcgtgg ataccctttc tggcggacaa cgccagcgcg cgtggatcgc gatggtgctg
    17041 gcccaggaaa cggcaattat gctgctcgac gaacccacga cctggctgga tatcagtcat
    17101 cagattgatt tgctggagtt gttaagcgaa ctgaaccgcg aaaaaggcta taccctggcg
    17161 gcggtgctgc acgatcttaa tcaggcctgt cgttacgcca gccatttgat tgcattgcgg
    17221 gaagggaaaa ttgttgctca gggagcgccg aaggagattg tcactgctga actgattgag
    17281 cgcatttatg gtctgcgctg catgatcatt gacgatccag ttgccggaac gccgcttgtg
    17341 gtgccgctcg gacgaacggc accttcaacc gcaaatagtt aaactaagtg gtctgccatc
    17401 atggcatcct gttttctgga tgccatcgca tagcgcaaca gcacgccacc acaagccacc
    17461 atcccgccga tcaacgcgga aaggatcaca ataatcgcct tacccggacc gtcttttttc
    17521 actggcaatg acgggcttaa ctgatattta aacggcgtaa aattcacatc gttgacatgt
    17581 gcttttgtta attgctcgac aagatactgc cgattacgta attcaccgtt cagttccgca
    17641 acgtcagtga ccgctttttc tatttccagt ttgcgttcaa taccgtctgc accgagagaa
    17701 atagaaaaat cggggtcatc tttaacggcc tgaccattac tgtaaacggg ctttttaatt
    17761 cctgccgcgt tggcaatgtc gagtgaataa ttgaggcgct gaatgtttgc atcaagttga
    17821 tttttcgttt taatgcgatc ctgagccagt ttttcttttt caaactgggt tttgatctcc
    17881 agtttattac ggacgttttc tagcgactct ttcaccacca acgtagagat ataatcgata
    17941 tacccgctca aaacggtctg cgcctcttca ctggttggcg cggtaaaact tagcgtccag
    18001 gaggtataca gtgacggttc atcttttttc ttactggcat tgtcatcaac cgctttcatt
    18061 ttttcgctca atgcgacaat tgcgcgatgc aaatccagtt cgtcgatttt cgcctctttt
    18121 aattggtcca tcacataagg tgatgaacgc aggtactctt ccagcaagct aaccgactga
    18181 aacttcttga taaacaggtt aaatgcttct gtacgatcaa ttttgatatc cagatccagc
    18241 acacgaagct tagtgaatgt tttctccaac tcttgccact gaacaggttc tggaggcgtg
    18301 acaaccgccg cgctggtcca tttttgcggc aggatgaaag agatcagcaa gcctgcgcag
    18361 gcaaacgcaa aaacgaccgc catgaccgtt tttttggccc gccataaaac tgagattaga
    18421 ttaagtaaat caatttcatt attactgggc gacgccagag gataatcggg aaaatgagcg
    18481 tcacttccct gtttaatatt cagtgatgac atggggtata acctgaaaat gagtccaata
    18541 gggggaaatc tccacggcgc tcactttacc agttggtgag aattatccga agctgaagtt
    18601 tgtaaattcc ttccaatgaa tgcaacattc actctattta agagaaataa ccagcaaact
    18661 tataaattaa acaaatgatt aacccataat tatttcctgt aattatgggt taatagataa
    18721 tgttaattta cctgtttagc gttgcgcgaa taatcggccc aattttttca aacgcccctg
    18781 gagagataat atccacatgc gcacaatcct gacgatagat atccagttcc gctatccacg
    18841 gcgaccaggc gcgttcggga ctcatacctt cctgaagtgt gcgttcagca acaaacagcg
    18901 tcgctttacc gtcaaacggt acgctatgag cagtcgtcag caggcgcaca gcatcagcgt
    18961 agttgccttc aatggtggta aacaactccg ttgaagtact tccctgctgt gctgccagga
    19021 aggcctcgcg ttcgcggtta atctccgcca gcacttccgg gtccagacca ttagcttctt
    19081 tttcctgcca gttttgcgtt tctggcggcc aggtatccag caagccaaga aatgccacct
    19141 gttcgccacg ggcacgcagt cgcgccgcaa taccctgcgc cagcgtaccg ccaagggaat
    19201 accccagcag gtaataaggg ccatgcggtt gttgttcaag taacgttgcc agatgcgctt
    19261 cgcagacttc atccaggttt gccgccgtct gcatggggcc attggggcgc ggtgactgaa
    19321 tgccgataat cgaccattgt ggatcgagat aacgcgagag cacgctgaac tgccaggcaa
    19381 aaccggacgc aggatggaaa caaaacagcg tcgggccatt accttcacgc aacggcagaa
    19441 tggtttcgaa tcccatacgc cgggtgctgt cttcttcagc atcaataatc gttgccagtt
    19501 tggcgacagt tgacgcgacc atcacttgcc ccggcgtcac ctggcgggca acctgccgac
    19561 ttaactgcgc tgccagtttc attgccagta gcgaatgacc gccaagcgcg aagaaatcag
    19621 catcggcatc ctgcacgtca caccccagca acgacgagaa tgccgcggcg ataatcgttt
    19681 cactgcccgc tttcggcgca cgccctggcg cttgtgcctt cagttcaggc aacggtaagg
    19741 ctttgcgatc cagcttgccg ttggcgctaa gtggtaactg tggaagttgc agcagaacca
    19801 ccggtaccat atgtggtggc aatgtttcac gaagctgcgc ctgcaatgcg ctggtatcca
    19861 acggcaggcc cgattgcgac accagatagc ccaccaattg acgcgcatca ccaccggtgg
    19921 cagccgcctg gttaatcaca caggcgtggg taacggcttg ttcgacatcc ggcagcgcct
    19981 gcatcacgcg atcgatttcg cccagttcaa tacgctgccc gcgaattttt agctgatcgt
    20041 cactgcgccc gaggtactcc accgcgccgt tatccagcca gcgggcaacg tctccggtac
    20101 ggtacatccg ttcaccagga gcaaaaggat cggcaataaa gcggctggcg gtcagatcgg
    20161 gtcgtccaag atacccctgc gccagttgaa taccggtgag atagagatct cccgccacac
    20221 ccggcggcac cggatgcatc atcgcatcaa gaatacgcag gcccgtgttc cacaccggat
    20281 aaccaatcgg cacactgctg ccgcgcacct gtgccagttc ctcgccaaaa gccggatacc
    20341 agctgacatc taccgccgct tccgtcgggc catatagatt atgcaacggc gcgccagtta
    20401 actgttgcca ttcgcggcat aaatcagccg gtaaggcctc accactacag aaaacctgtt
    20461 tcaacgtcgc gcaactctgg cgagcggttt gcggcgtcag cgaggcaaca aatgccgcca
    20521 gcatcgacgg cacaaagtgc gtggtcgtta cgccatattc ggcaaagaat tgctgcatag
    20581 cgagcgggtc gcggtgcgct tccggttcag ccatcaccag ttttgcccct gcgataaacg
    20641 gccagaaaaa ctcccacacc gagacatcaa aactgcacgg cgttttttgg gcaacgacat
    20701 cttcgcctgt aagcggataa tgattttgca tccaaagcag gcggttgacg atagccgtct
    20761 gcccgaccat tacccctttc ggcctgccgg tggagccaga ggtaaagatg atataagccg
    20821 tgtgatgcgg ttgtgaaagt tgcagcggcg cactgccctg cggtgtaagc ggggcgttat
    20881 agcaaaggtt ggttaaatct ggaacatcgg caaagcgcgg cagttgatcg tcggtggtga
    20941 ttaacagcga cggacgcgca tcttctagca tcattttcag gcgatcgtcc ggataaccgg
    21001 tatccagcgg tagccaggcc gcacctgctt caacaatcgc atgtagcgcc agggtcaaaa
    21061 agaccgagcg cggtagcgcc accgctacgc tgtcgcccgg tttaacgccg cgctcacgca
    21121 gcagattcgc cagcgccacc acctgctcac gcatttcccg atagctgaac tggtaatgcg
    21181 catctgccag cgccggagca tccggagttt ttgccgcttg ttctgccacc agagcgctaa
    21241 gcgtggtttc tggaatctca acctgagtgg cgttgatctg cgccagctgc gcgtattcac
    21301 ctggcagcat aatatcgaca tcaccgcaca acagcgccgg atctgcggcg aactgggcaa
    21361 tcagcatttt caggcgttca gcatgctgga ttaacgttgg ctcagcgtaa cgctgtttat
    21421 tggcgaggat ctcaatactc aaatcaccgt gtacatccgg gaacagggcc agttcaaggt
    21481 cattaaccgg accggttgcc agggtatggg tttgcgcctg aacatcagga atatccagtt
    21541 ggtaatcaaa taccttgata ttgagtaccg gaccaaacag cggttcatca cctgccgctc
    21601 gcccgctgtc acggacaatt tgttcggcat cgtaacgttg atgacgacgc atttttttca
    21661 gttgtgctgc cagtcgggtt gccagttccg gcagcgtttc ttgcgccgca atgtgaatac
    21721 ccaacggcaa aacgttgagc acgggtccgg tagccgtcag cgccgccgag cccagtcgac
    21781 gcataaagat aaatccggcg gcgtagtcca tacgattgca caatcgcccc agccacaagg
    21841 ctgccagcgc aagggctaaa tcggtacgct gcacacctga aagttgcgta gccagctggc
    21901 ggaattcccc gtcggtaaat tccagtttca ggcgcagaat atctgccgag gcgctgcgcc
    21961 ccggtaaagg tgccggagaa agtgacgcgg gcggcggcag ttgacgacgc tgttctgccc
    22021 agaatgccgc atcacgctgc caggcttcgc tttcgcggta ttgctggtac tcttccacta
    22081 catcagcgaa aggcgtaaac ggcgaagcag gcgttggttc gccacgcagc catgtgcagt
    22141 aaatattggc gatctggcga gtaatggccg ggaaactgaa gccatcgacc agcaaatggt
    22201 gataacgctg ataccagtac cagcggttat ccgcaacatg tatcagctga tgaaagacca
    22261 gtggtttacc gctatcgacg cgcaaatctt gttgcaaatc cgccagcatt aatgcctgcg
    22321 cagtaccgtg cggatcaata ttggttcgca ggtcgataat ttctggcagt tcgaatatca
    22381 gcgcatcatc gacccactgc cagacttcgc cgttatcttc cgtaaaacgc atccgcaacg
    22441 tatctgcttg cgctagtccg acaaccaccg cgcgggccag taatggcgca tcaacatctc
    22501 cagttaactc aacgtaatgc gccacgctcc aggcggaggg taaatccgac agtttttctg
    22561 ccatccagat gccgggctgt gcggcgacca aaggtaaatg ctggctcatt gtgcctcctg
    22621 caactgggtg aaattggccg gtgtcagagt acgccagtgg acttccagcc actgctggca
    22681 ggacgcctgt gactgcggct gacacacaat gtcccagcct gccggtaagg cgcattgttg
    22741 cggccacaga ctgaattgcc cctgcgcatt gcgcaaaatg taaaacgctc cctgcggatc
    22801 atcgaaggga ttactgaatg ccatattcaa ctcctgtcat ggaaaagtgg ttgccagagg
    22861 tcgattagcc cctgcatcaa gccaccgcgc caacaaagcg catcatgtcc gccgtcaacc
    22921 tgacgccaga aaatggattc ttttatgggg tgtaattgcg catacagcgc ctgattggcc
    22981 cgcattatca tcggctcgcg aatacccgct tccagcacaa tgcgcagacc ttcggcacta
    23041 acttgaccag ctttaagttg ctcaagtaaa acaccttctt gctgcccgcc ccgatgcggc
    23101 caccagtacg atcctgactg gcttaataca cagccaaagc gttcaggcca gtgcagtccg
    23161 gcatacagcg cggaaagtcc accaaaactc tgcccggcga ccacggtgcg atcggcgcga
    23221 tcgctaaaag gggcaatagc tttcaccagg ggtaataact cttgctgtac tgccagccag
    23281 aaatccgcat tacacggcag ttcgtgagcg cggtgcgtag tgtcgatagc gtcgatcaac
    23341 acatacacgg cgggaggaag ttgctgacga tgggtcaacg aagtcagcac aggccagaca
    23401 ggcatactct gcgcccaaaa ttcgccatcg agcaaaactg ccagcgggcg ttctcctgct
    23461 gttgcatcgc cagtggtaaa aatccataca cggcgtgaat ttttcaaccg ttcacttttc
    23521 cagataattt ctttggcagg cgtttctggc gcttgcggac aatcccatcc cggttgcaga
    23581 ggcgcttgcg gcatttcgag tgcagaaaca gcgtgccctc gcccgccttt ccagctttgt
    23641 ggattcagcg gatcggctat tgcctggggt aatagttttc gccagccttc acgtaattcg
    23701 aggcgatcgg gggatggtgc agaaaaaatg tcatcgcgtt cggtgggaat aaaacagtag
    23761 ctgccgcgcc agttggcatt gagttgtgtc gtccactgcc agacgttggt gcctgcaatt
    23821 cgctgcatcg actggggctg gctgttctga tggtgatcgg tcacaccagt gatgtagacc
    23881 catacgcgct ttatcgtcga gtattcttca gaaccttggg gatcacgcca ccagaaagtg
    23941 acctcaaaca tttcgtcatt cagacgctgc cattccgggc catgtttcga ctgccaccag
    24001 ctctcacttc ctacttttaa cgccgtcaca ccataacccc atgtttactg tgcaattttt
    24061 cattgattgc agaaatatat tgataatatt attgataact atttgcattt gcaatagcgt
    24121 aatggcgcgc cgtgggaagc gcggacatta attaaccaac tgcactgcgt gtctttcagg
    24181 atcaaaggtt ttcgcggtag cgggatgcgt cgtattgatg acgaccatgc ccgacagttg
    24241 caattcgtgg caaaaatgca ggaataaaac aatgaacaag aagattcatt ccctggcctt
    24301 gttggtcaat ctggggattt atggggtagc gcaggcacaa gagccgaccg atactcctgt
    24361 ttcacatgac gatactattg tcgttaccgc cgccgagcag aacttgcagg cacctggcgt
    24421 ttcgaccatc accgcagatg aaatccgcaa aaacccagtt gcccgcgatg tgtcggagat
    24481 catccgtacc atgccaggcg ttaacctgac cggtaactcc accagtggtc agcgtggtaa
    24541 taaccgccag attgatattc gcggcatggg tccggaaaac acgctgattt tgattgacgg
    24601 caagccagta agcagccgta attcggtgcg tcagggctgg cgtggtgagc gcgatacccg
    24661 tggtgatacc tcctgggtgc cacctgaaat gattgaacgt attgaagttc tgcgtggtcc
    24721 ggcagctgcg cgttatggca acggcgcggc gggcggcgtg gttaacatca ttaccaaaaa
    24781 aggcagtggt gagtggcacg gctcctggga tgcttatttc aatgcgccag aacataaaga
    24841 ggaaggtgcc accaaacgta ccaacttcag cctgaccggt ccgctgggcg acgaattcag
    24901 cttccgcttg tatggcaacc tcgacaaaac ccaggctgac gcgtgggata tcaaccaggg
    24961 ccatcagtcc gcgcgtgccg gaacgtatgt cacgacgtta ccagccgggc gcgaaggggt
    25021 gatcaacaaa gatattaatg gcgtggtgcg ctgggacttc gcaccattgc aatcgctgga
    25081 actggaagcg ggttacagcc gccagggtaa cctgtatgcg ggcgataccc agaacaccaa
    25141 ctctgacgct tacactcgct cgaaatatgg cgatgaaacc aaccgcctgt atcgccagaa
    25201 ctacgcgctg acctggaacg gtggctggga taacggcgtg accaccagca actgggtgca
    25261 gtatgaacac acccgtaact cgcgtattcc ggaaggtctg gcgggcggta ccgaagggaa
    25321 atttaacgaa aaagcgacac aggatttcgt cgatatcgat cttgatgacg tgatgctgca
    25381 cagcgaagtt aacctgccga ttgatttcct cgttaaccag acgctgacgc tgggtacgga
    25441 gtggaatcag caacggatga aggacttgag ttccaatacc caggcgctga ccggggcgaa
    25501 caccggcggc gctattgatg gtgtgagtgc caccgaccgt agcccgtatt caaaagcaga
    25561 aattttctcg ctgtttgccg aaaacaacat ggagctgact gacagcacca tcgtaacgcc
    25621 ggggctgcgt ttcgatcatc acagtattgt cggcaataac tggagcccgg cgctgaacat
    25681 atcgcaaggt ttaggcgatg acttcacgct caaaatgggc attgcccgtg cttataaagc
    25741 accgagcctg taccagacta acccgaacta cattctctac agtaaaggtc agggttgcta
    25801 tgccagcgcg ggcggctgct atctgcaagg taacgatgac ctgaaagcag aaaccagcat
    25861 caacaaagag attggtctgg agttcaaacg cgacggctgg ctggcgggcg tcacctggtt
    25921 ccgtaacgat tatcgcaata agattgaagc gggctatgtg gctgtaggac aaaacgcagt
    25981 cggcaccgat ctctatcagt gggataacgt gccgaaagca gtggttgaag gtctggaagg
    26041 atcgttaaac gtaccggtta gcgaaacggt gatgtggacc aataacatca cttatatgct
    26101 gaagagtaaa aacaaaacca cgggcgaccg tttgtcgatc atcccggagt atacgttgaa
    26161 ctcaacgctg agctggcagg cacgagaaga tttgtcgatg caaacgacct tcacctggta
    26221 cggtaagcag cagccgaaga agtacaacta taaaggtcag ccagcggttg gcccggaaac
    26281 caaagaaatc agtccttaca gcattgtggg tctgagcgcg acctgggatg tgacgaagaa
    26341 tgtcagtctg accggcggcg tggacaacct gttcgacaaa cgtttgtggc gtgcgggtaa
    26401 tgcccagacc acgggcgatc tggcaggggc caactatatc gcgggtgccg gggcgtatac
    26461 ctataacgag ccgggacgta cgtggtacat gagcgtaaac actcacttct gatgctaacg
    26521 tcagattgtt gacaaagtgc gcgtcgttca tgccggatgc ggcgtgaacg ccttatccgg
    26581 cctacaaaaa tcttgccaat tcaatatatt gcaggaccat gtaggcctga taagcgtagc
    26641 gcatcaggca gtttagcgtt tgtcatcagt ctctaatatg gtcgacatga aaactacgca
    26701 tacctccctc ccctttgccg gacatacgct gcattttgtt gagttcgatc cggcgaattt
    26761 ttgtgagcag gatttactct ggctgccgca ttacgcgcaa ctgcaacacg ctggacggaa
    26821 gcgcaaagcc gaacatttag ccgggcggat cgctgcaatt tatgcgctgc gggaatatgg
    26881 ctataaatgt gtgcccgcaa tcggcgagct acgccaaccg gtgtggcctg cggaggtata
    26941 cggcagtatt agccactgcg gggctacggc attggccgtg gtttcccgtc aaccgattgg
    27001 cgttgatatc gaagaaattt tttctgcgca aacagcgacc gaattgacag acaacattat
    27061 tacaccagca gaacacgagc gactcgcaga ctgcggttta gccttttctc tggcgctgac
    27121 actggcattt tccgccaaag agagcgcatt taaggcaagt gagatccaaa ctgatgcagg
    27181 ttttctggac tatcagataa ttagctggaa taaacagcag gtcatcattc atcgtgagaa
    27241 tgagatgttt gctgtgcact ggcagataaa agaaaaaata gtcataactc tgtgccaaca
    27301 cgattaattg acaacatctg gtacgattcg cccgcagcca tcactgaccg cgggcgaaag
    27361 tgtaaagcag gtgccttacc actctgacct gacaaccgga tatgcgggga ttgctccccg
    27421 cacaacggct acttcttcgg ttcgtaagcg agaacagcct taaactcaat attacgttcc
    27481 tttacggtga actcacacag cgagtctccg accagaagcg taaatcccag taccgtcaaa
    27541 cacagcacta tgactgccac aagggcatat ttcgtcagca tgtttatatt gcctccatgc
    27601 gaagaaggga ctaccatccg agttgttgag gctcggagtg gcagccccag gattgataga
    27661 aatatctcct ggggcttcat ctttccagac ctcagagtta acctgaaacc ggaaagcttc
    27721 aggcacccgc cgttatctta cccttcctca acaaaatagc tgtttattta tacagtgaat
    27781 cctgtttcgg acttctgtcg gcaaaactca tcattgctct taaaacgaaa tcgcccgcag
    27841 ccatcactga ccgcgggcga aagtgtaaag caggtgcctt accatcctga cctgacaacc
    27901 ggatatgcgg ggattgctcc ccgcacaacg gctacttctt cggttcgtaa gcgagaacaa
    27961 ccttaaactc aatattacgt tcctttacgg tgaactcaca cagcgaatcc ccgaccagaa
    28021 gcgtaaatcc cagcaccgtt aaacacagca ctatgactgc cgcaagggca tatttcgtca
    28081 gcatgtttat attgcctcca tgcgaagaag ggactaccat ccaaattgtt caggtttggg
    28141 taggtagccc cagggttgat agaaatatcg tcctggggct tcatctttcc agacctcaga
    28201 gtaaacctga aaccggaaag cttcaggcac ccgcagttat cttacccttg cttagcaaaa
    28261 tagctgttta tttatacagt aagtcctgtt tcggacttct cactccccat ttgatcaact
    28321 gaagctgaac ccgttttttt ctgcatgcct caatcgtcaa aattcttcgc caccagccag
    28381 ccgtgccata acttgtcaca ttgcaaaagt agccaaattg cttcagtcac catcaccaaa
    28441 aaaagaaaca ttccagaact tccgcatctc gcccagccgc tccgacttct atactgaata
    28501 gaaaacgcca acttaagaga aacctatgcc attacccgat tttcatgttt ctgaaccttt
    28561 taccctcggt attgaactgg aaatgcaggt ggttaatccg ccgggctatg acttaagcca
    28621 ggactcttca atgctgattg acgcggttaa aaatgagatc acggccggag aggtaaagca
    28681 cgatatcacc gaaagtatgc tggagctggc gacggatgtt tgccgtgata tcaaccaggc
    28741 tgccgggcag ttttcagcga tgcagaaagt cgtattgcag gcagccgcag accatcatct
    28801 ggaaatttgc ggcggtggca cgcacccgtt tcagaaatgg cagcgtcagg aggtatgcga
    28861 caacgaacgc tatcaacgaa cgctggaaaa ctttggctat ctcatccagc aggcgaccgt
    28921 ttttggtcag catgtccatg ttggctgcgc cagtggcgat gacgccattt atttgctgca
    28981 cggcctgtca cggtttgtgc cgcacttcat cgccctttcc gccgcgtcgc cgtatatgca
    29041 gggaacggat acgcgttttg cctcctcacg accgaatatt ttttccgcct ttcccgataa
    29101 tggcccgatg ccgtgggtca gtaactggca acaatttgaa gccctgtttc gctgcctgag
    29161 ttacaccacg atgatcgaca gcattaaaga tctgcactgg gatattcgcc ccagtcctca
    29221 ttttggcacg gtggaggttc gggtgatgga taccccgtta acccttagcc acgcggtaaa
    29281 tatggcggga ttaattcagg ccaccgccca ctggttactg acggaacgcc cgtttaaaca
    29341 tcaggagaaa gattacctgc tgtataaatt caaccgtttc caggcctgcc gctatgggct
    29401 ggaaggcgtc attaccgatc cgcacactgg cgatcgtcga ccgctaacgg aagatacctt
    29461 gcgattgctg gaaaaaatcg ccccttccgc acataaaatg ggtgcatcga gcgcgattga
    29521 ggccctgcat cgccaggtcg tcagcggtct gaatgaagcg cagctgatgc gcgatttcgt
    29581 cgccgatggt ggctcgctga ttgggctggt gaaaaagcat tgtgagatct gggccggtga
    29641 ctaagtaggg aattgcctta cagaagaata tccctgacaa tggtgtttta acttacattt
    29701 aacaatgtta tgaaacaccc tttagaaacc ttgactaccg cagcaggcat tttgctgatg
    29761 gctttcctct cttgcctgct gctgcccgcc cccgcactgg ggctgacgct ggcacaaaaa
    29821 ctggtgacca tgttccatct gatggatctt agccagcttt acactttatt gttttgtctg
    29881 tggtttttag tgctgggcgc tattgagtat tttgttctgc gctttatctg gcgacgctgg
    29941 ttctcgctgg cggattaaac gtcgattatc gccgtacttc gcggcatact ttgcttattc
    30001 tccttcgcct aaaggaacgt ttatggataa gcaatcactg cacgaaacgg cgaaacgcct
    30061 ggcccttgag ttaccctttg tcgagctttg ctggcctttt ggcccggagt tcgatgtttt
    30121 taaaattggc ggcaaaattt ttatgctgtc gtcggagctg cgcggcgtcc cctttatcaa
    30181 tctgaagtcc gatccacaaa aatccctgtt aaatcagcag atatacccga gcattaagcc
    30241 agggtatcac atgaataaaa agcactggat ttcggtgtat cccggcgagg aaatctccga
    30301 agcgttaatt cgcgatctga tcaacgattc gtggaatctg gtggttgatg gtctggctaa
    30361 acgcgatcaa aaaagagtgc gtccaggcta aagcggaaat ctatagcgca tttttctcgc
    30421 ttaccatttc tcgttgaacc ttgtaatctg ctggcacgca aaattacttt cacatggagt
    30481 ctttatggat atcatttctg tcgccttaaa gcgtcattcc actaaggcat ttgatgccag
    30541 taaaaaactt accccggaac aggccgagca gatcaaaact ctcctgcaat acagcccatc
    30601 cagcaccaac tcccagccgt ggcattttat tgttgccagc acggaagaag gtaaagcgcg
    30661 tgttgccaaa tccgctgccg gtaattatgt gttcaacgaa cgtaaaatac ttgatgcctc
    30721 gcacgtcgtg gtgttctgtg caaaaaccgc gatggacgat gcctggctga agctggttgt
    30781 tgaccaggaa gatgctgatg gccgctttgc cacaccggaa gcgaaagccg cgaacgataa
    30841 aggtcgcaag ttcttcgctg atatgcaccg taaagatctg catgatgatg cagagtggat
    30901 ggcaaaacag gtttatctca acgtcggtaa cttcctgctc ggcgtggcgg cgctgggtct
    30961 ggacgcggta cccatcgaag gttttgacgc cgccatcctc gatgcagaat ttggtctgaa
    31021 agagaaaggc tacaccagtc tggtggttgt tccggtaggt catcacagcg ttgaagattt
    31081 taacgctacg ctgccgaaat ctcgtctgcc gcaaaacatc accttaaccg aagtgtaatt
    31141 ctctcttgcc gggcatctgc ccggctattt cccctcagat tctcctgatt tgcataaccc
    31201 tgtttcagcc gtcatcatag gctgctgttg tataaaggag acgttatgca ggatttaata
    31261 tcccaggttg aagatttagc gggtattgag atcgatcaca ccacctcgat ggtgatgatt
    31321 ttcggtatta tttttctgac cgccgtcgtg gtgcatatta ttttgcattg ggtggtactg
    31381 cggaccttcg aaaaacgtgc catcgccagt tcacggcttt ggttgcaaat cattacccag
    31441 aataaactct tccaccgttt agcttttacc ctgcagggga ttatcgtcaa tattcaggcg
    31501 gtattctggc tgcaaaaagg caccgaagcg gcagatattc tgactacctg cgcgcagttg
    31561 tggatcatga tgtatgcgct gctttcagtc ttctcgttgc tggatgttat tttgaatctg
    31621 gcgcagaaat tcccggcggc atctcagtta ccgctgaaag ggatatttca ggggattaaa
    31681 ctgatcggcg caatcctggt cggcattttg atgatctcgc tgctgattgg tcagtcgcca
    31741 gcgattctga tcagcggtct tggtgcaatg gctgccgtgc tgatgttggt ttttaaagat
    31801 ccgattcttg gtctggtggc aggtattcag ctttccgcga acgatatgct gaaactgggc
    31861 gactggctgg agatgccgaa atacggcgcg gatggcgcgg tgatcgatat tgggttaacc
    31921 accgtcaaag tgcgtaactg ggacaatacc attaccacta ttcccacctg gtctctggtt
    31981 tctgactcct ttaaaaactg gagcgggatg tcagcatctg gcgggcgacg tattaagcgc
    32041 agtatcagta ttgatgtcac cagtattcgt tttcttgatg aagacgaaat gcaacgtctg
    32101 aataaagcgc acttgttaaa accttattta accagccgcc atcaggaaat taatgagtgg
    32161 aatcgccagc aaggttctac ggagtcgata ttaaacctgc gccgaatgac caatattgga
    32221 acctttcgtg cctatctgaa cgaatatctg cgtaaccatc cgcggattcg taaagatatg
    32281 accttaatgg tacgccaact ggctccaggt gataacggtt taccgctcga gatctacgcg
    32341 tttactaaca ccgtggtgtg gctggaatat gaaagcattc aggctgatat attcgatcac
    32401 atatttgcga ttgtcgaaga gtttggtctg cgacttcatc agtcgccaac cggcaatgat
    32461 attcgctctc tggcgggtgc atttaagcag taattaaaaa aaccgctctc atcgaatgga
    32521 tgagagcgga catcgttttg tcggatgtag cgtgaatgcc ttatttccga cgcagcgttt
    32581 taaatgccac aaacaggaat acaatccaca ccggcagcag gatcgctgac aagcgcatat
    32641 catccatcgt gcacatcagc agcaaaatca tgccgaggaa ggcaatgcaa agatagttgc
    32701 cgaacggata gagcagcgcc ttaaactgtg tttcacgccc ctgacgtcgc atcgctgcac
    32761 gaaaacgcag atgcgccaga cagatcataa tccagttcaa cagcagcgtt gctaccacca
    32821 gcgccatcag cagaccaaac gctttttgcg gcagcagata gttgattaat accaccagcg
    32881 aagtgatcgc tccggaaagc atcagcgagt taatcggcac accgcgacgg ctgacgcgag
    32941 tcaaaaactt cggcgcatta ccctgcacag aaaggccaaa cagcatgcgg ctgttagagt
    33001 aaaccccgct gttatacact gacagcgatg ctaccagaat gacgaagttc agcgcagaag
    33061 ctaccacgtt gctgtcgaga ttatggaaaa tcatcacaaa cgggctactg ttggatttca
    33121 cttccaccca cggatagagc gccagtaaaa ccaccagtga accgatgtaa aacagcagga
    33181 tgcgatacac cacctgattt accgcttttg gaatgctttt ttccggatcg cgcgcttcag
    33241 cggcagtaat cccaatcagc tccagaccgc cgaaggagaa cataattacc gccagcgaca
    33301 aaatcagccc attccagccg gtggcgaaga aaccaccgta gcgccagagg ttgtcgatac
    33361 tggctttctc gccgccgtga ccagaaaaca gcagccacag gccaaagccg atcataccga
    33421 tgatcgccag cactttaatc agcgcaaacc agaactcggt ttcaccatat aagcgcacgt
    33481 tcaccaggtt aacggcgttg atgataataa agaaggcggc agcccaaatc cacgttggaa
    33541 catccgggaa ccagtactgc atatagatgc ccgcagcggt cagctctgcc attcccacca
    33601 gcacgaacat tacccagtag ttccagccag agaggaagcc cgcaaacggt ccccagtatt
    33661 tataggcaaa gtgggcaaat gaaccggata ccggctcctc aaccaccatt tcgccaagct
    33721 ggcgcataat caggaaagcg atgatcccgg cgacgccgta gcccagcaat acagccggac
    33781 ccgccatctg aatcgccggg ccaatgccaa gaaacagacc agtaccaatt gcgccaccca
    33841 acgcaatcag ttgaatatga cggttatgta atccgcgatg aagcgtcggc tcttgattcg
    33901 acgcagtatc ttccgatacg gttgacgcgt ttttcacgcc tttcccctgt gtgtcttttt
    33961 tgttgagggg caccttttaa catttagtgc ccatcgtcgc aagacacaat ccacacggtt
    34021 aaacggggta tcctgctttt atttccgtac ccgatgtcgg tgcagccaca tcagcttata
    34081 cgccgccggg ataataaaca gcgacagcaa aggtgcggtg atcatgccgc caatcatcgg
    34141 tgcggcaatc cggctcatca cctctgaacc agcccccgtt ccccacagaa tcggcagcag
    34201 accggcgata atcaccgcca ccgtcatcgc tttcgggcgc acgcgcagga ccgcgccgtg
    34261 atataacgcc tcatccagct tctgctcgct gaatgtttgt ggattattca acgacggcac
    34321 ggcctctatg gcgtgacgta aatacatcag catcaccacg ccaaattcgg cggcgacccc
    34381 ggcgagggcg ataaagccag tgcccgtcgc cacggaaaga tgaaagccca tccaccacag
    34441 caaccagatg ccgcccacca gcgcaaacgg tacgctgctg ataatcagca acgcttcgcc
    34501 cacccgacgg aacgccagat acaacagcac gaagataatc atcaacgtca tcggcaccat
    34561 gagtttaagc ttatggttgg cgcgctccag tagctcgaac tgcccggaga atgccacgct
    34621 ggtgcccggt tttaactgca ctttttcagc tatcgctttt tgcaaatcgt gaaccaccga
    34681 caccatgtca cgatcgcggg catcgatata aatccagctc gtcgggcgcg cattctcggt
    34741 tttcagcatc gacggtccgg tagagacttt aatgtcggcc acgtctgcca gggtgatttg
    34801 ctgcttcatc ggcgtcagga tcggcagctg gcgcagtgcc tgcggactat cgcgccagct
    34861 ttgcggataa cgcagattaa ttggataacg ggcaatccct tccaccgttt cgccaaccat
    34921 cgccccgccc accgcagaag tcacaaacaa ctgcacatcc gccaccgtca taccgtaacg
    34981 tgcggctttt tcacggttaa tctcaacgtt gatatagcgc ccaccttcca gccgctcggc
    35041 aagcgcagaa gctacgcctg gcaccgttcg cgctacttct tcaatttgct cagccatcgc
    35101 gtcgatatcc gccagcacag tgccggaaac tttaatgccg atggggcttt taatgccggt
    35161 tgagagcata tcgatacggt tacgaattgg cggcacccac agattcgcca gccccggcag
    35221 ccgcacggtg ttatccagtt cctcaatgat tttgtccatc gtcatgcctg gccgccactg
    35281 ctcctgcggc ttaagctgga tggtcgtttc taccatctcc agcggagcag aatcggtggc
    35341 ggtttccgct ttcccggttt tgccaaatac ccgcgccact tcaggtacgc tcataattag
    35401 cttgtcggtt ttttgcagca tactcgccgc ctctgctgcg gaaatccccg gcagcgtcga
    35461 tggcatatac aacaagtcgc cttcattgat ctgcggtaaa aattccccgc caactttatt
    35521 gagcggccag agaaccgtca gcaccgaaag cgccgccacc agcagcgtgg ttttcggcca
    35581 gtgcagtact ttcagcaaca gcggatgata aacacgaatc aaaaagcgat tgagcgggtt
    35641 actgctttcc ggcggaattt tgccacggat ccagtagccc atcaggatcg ggatcactac
    35701 gatcgccagc agcgccgcac ccgccatcgc atacgttttg gtgaacgcca acgggccaaa
    35761 cagacgccct tcctgccctt ccagggtgaa gatcgggata aacgacaacg tgataatcag
    35821 cagactgata aatagcgccg gccccacttc aacagacgca tcggtgatca cctgccagcg
    35881 cgttttatta tccagcgtgg cgtcaggatg ctggtgctgc cactcttcca gccgtttatg
    35941 tgcattttcg atcatgacga tagcggcatc gaccatcgcc ccgacggcaa tcgcaatgcc
    36001 acccaacgac ataatattgg cattcagtcc ctggaagtgc atgacaataa aagcaataca
    36061 caaccccagc ggcaacgaaa taatcgccac cagcgccgag cgcacatgcc agagaaacag
    36121 cgcacagact accgccacca caataaactc ttccagcaac ttgccgctga ggttgtcgat
    36181 ggcgcggtca atgagctggc tgcgatcgta tgttgtaact atctccacgc cttccggcag
    36241 actacttttc agcgtttcca gtttgtcctt cacggcggcg atcacttctc gggcgttttt
    36301 gccggatcgc aggatcacca ccccgcccgc cacttcgcct tcgccgttta gttcggcaat
    36361 gccccggcgc atctccgggc caatctggac cttcgcaaca tcgcgcagat aaacgggcac
    36421 gccattttca ctggctttta aaacgatgtg attaaagtcg tcgagcgttt gcagatagcc
    36481 gctggcgcgc accatatatt ccgcttccgc cagttcgatc gacgaaccgc ccgcttcctg
    36541 gtttgaagca tccagcgcgc tttttacttc ggcgagactg atgccatact gcgccaggcg
    36601 ctggggatcg ataaccacct gatactcttt caccacaccg cccaccgacg ccacttccgc
    36661 aacgtcaggg atggttttca gctcatattt gagaaaccag tcctgtaatg agcgtaaatc
    36721 ggccagatcg tgcttaccgc tgcgatccac cagtgcatat tcatagatcc agccaacacc
    36781 cgtggcatct ggccccagct cggcgctgac tcccgcaggc agcttaccct gtacctggtt
    36841 gaggtactcc agcacccgcg aacgcgccca gtacggatcg gtgccatctt cgaaaatgac
    36901 atacacataa gagtcgccaa actgtgagaa accgcgcaca gtcttcgcgc caggcaccga
    36961 caacatggtg gtggttagcg gataagtcac ctgattttca acgatttgtg gtgcctgacc
    37021 gggatagctg gttttaataa taacctgcac atcggagaga tccggcagcg catccactgg
    37081 cgtattaatg atggtccagg tgccccagat gctcagaaac aacgcgccca tcagcaccaa
    37141 aaaacggttc gccaccgagc gacgaataat ccattcaatc attggttatt ccctcaatgc
    37201 gcatgggtag cactttcaga gcgcatccgc tccagtgcgc cagaaatatt ggcttcggaa
    37261 tcaatcagga acaggccgct ggaaaccacc ttttcacctt ccgccagacc agagcgtaat
    37321 gcggtgacgc cttgcgatgc ctggaaaaca gcaacgcgtt tcggtacaaa gcgcccgtcg
    37381 gcatcaacgg taatcacccg ctgttcgctg ccggtatcaa tcagcgcttg tgacggaatg
    37441 agcagcatcg gttcgctggc ggtgttgagt tgcaaccagg cgttcatgcc cggttttagc
    37501 gcctcgtcgg cgttgtcgac ttccagacgc agctgcagcg tgcgggtcgc ggcatccacg
    37561 ccaggtagca gcgtccattt gcggatggtg agtgttttat ccggtcgcgc cggaacggtg
    37621 agggtaaact gcgaggcatc tttcaccagc caggcgatag actccgggat cgcagcagtg
    37681 acccacaccg ggtccatacc ctgaattttc gctaccacgt tatctttggc gatattcatt
    37741 cccgcgcgca gatcaaacgc ggtgatcacg ccatcaatgg gcgctttgag cgtaaagcga
    37801 gtctggattt tttgcgtggc gatcaggcgg cgaatatccg cctccggcat tcccgccagt
    37861 cgcagccgct caagaatgcc ttcagtctgg gtcgccgtac cgccggtttc gcgcagcagt
    37921 aaatactcac tctgcgcttc cacccagtca ggaatggtca ggtcgagaag cggtgtgccc
    37981 ttttgtactt tatcgcccac ggtaagcgga tacaccttgt cgataaaccc ggcagcgcga
    38041 gcctgcacaa tggcatactg atactcgttg taactgacat tcgccgggaa actctgggca
    38101 aaagtcagcg gcccgcgcgt gacggtagcc gttttcaccc ccagattctg cgtctgagtc
    38161 gggtcaatgc gcacaccaga cgcagaactc tcttcatcgg catatttcgg caccagatcc
    38221 atatccataa acggcgattt acctggttta tcgaaccgcg tattggggta catcgggtcg
    38281 taccagaata agattttacg ttctgcggtc gacgtttttt ctgcgggtgg ttcagccttt
    38341 gcaaaccagg taaaacctgc cgcagaaata ataccgcccg cgatcatgct gccgataata
    38401 agcgcgattt ttttcatctc attaaacctg ggttactggc tgactttaat atcctgtaat
    38461 aaagaaaggt tgccctgctg gacaaaatta aacgccactt tgtcgccagt tttaatttca
    38521 ctcattttcg tctgcggagt gatggtaaag cgcatggtca tctccggcca gttcacggca
    38581 gcaatcggat cgtgatggat ggtgattttt ttgctttcca gatcaatacc ctttaccacg
    38641 ccagtggcgc taataacctg cggttgtact tcgctcatgg tttcatgatg atgttcgtta
    38701 gcctgggcat taaagccaat aacggtaaac agactgaaca ttgcgacttg cagtgctttt
    38761 ttcatttatt ctctcctgga gttaaaaatt attgctgcca accgccccct aaggcggtat
    38821 acaaagaaat ttcgttaacc tgacgggcat aattcaggtc gagtaaagtt tgtcgggttg
    38881 caaataaaga acgctcggca tccagcactt ccagataact taccgcgccg tgctgatata
    38941 atgcccgcgc ccgttgcaaa gtaatttgca gcgacgccag ataacgctgc tgggcgctga
    39001 tttgatcgtt caggctttga cgtaatgcca gcgcatctgc cacttcttta aaggcgttct
    39061 ggattttctg ttcataattc actaccgact gctgctggcg aatttcggcg atatccagat
    39121 tggcttggtt gcgtccggca ttaaaaatgg ggatctcaat tttaggaata aaattccaca
    39181 tcccgctgct agcgttaaat aatgaagaca gatcgctgct ggcggttgat attccgctgg
    39241 tcaggctgat agacgggaaa aatgccgcac gcgcagcgcc aatattggca ttagccgcca
    39301 ttaacgcgtg ttcagcttcc ataatatccg ggcgctgcaa taagatttgc gacgacaatc
    39361 ccgccggtaa tttaacgctt tgcaggctgt cgctgtttac tgtctgcgct tgcggcagct
    39421 tgccgtagct tcccaataac agttgcaatg cattattcgc ctgcgccagt tccccctgac
    39481 gtttagcgat gtcgctgcgg gtactttcta tcaccccgcg agcctgttcc agcgccagaa
    39541 cattactgct acctgtcaac agttgttttt cgacaaacgc atatgactgc tgataattac
    39601 gcagcgtttc ttcggctatt tgcaattgcg catacgccag ttgctgattg aaatagcttt
    39661 gcgcgacatt agaaaccagc agaatatgca ccgcgcgctg agcttcctca gtggctaaat
    39721 aattttgtcg ctcggcttcg ctcatgttct ttaagcgacc gaaaaaatcg aggtcaaagc
    39781 tggcgttaag gccggtcgaa aactcccgtg tcgtggcggt attcccttta agattaccgc
    39841 tccagctgcc gctgccctcg ccattgagct gtgggtagcg gtcggcatcg gtcagacgat
    39901 attgcgcccg tgcctcctgc actttcagcg tcgccatgcg caaatcccga ttattcacca
    39961 gcgcctcgct aatcagcgtc ttcacctgat tatcaacaaa aaaggtgcgc cagcccgcgt
    40021 tctgatagtt atctgctgcg ttaaccaggc cgttctggct gagtgagaac tgctgcggca
    40081 cgggcattgc cggacgctga taatccggtg ccagtgaaca accggttagc gcaagggcca
    40141 cacaaaatgg cagaagttta caaggagaca taggctcata atttctggtg attttatgcc
    40201 gccaacttta ctcgccaggc tctgattttc cggtgacaga aaaatgacaa aattgtcatt
    40261 ttgccaataa gcgattgcca tctgatcccg ctactctaga attgcccggg caacatgcgg
    40321 aggaaatatg aaactgttga ttgtcgaaga tgaaaagaaa accggagaat acttgaccaa
    40381 agggttaacc gaagccggtt ttgtggtcga tttggccgac aacgggttga atggctacca
    40441 tctggcgatg accggtgatt atgatctgat aatcctcgat attatgctgc cggacgtgaa
    40501 cggctgggat atcgtgcgca tgttgcgttc cgccaataaa gggatgccga ttctgttgct
    40561 taccgcgctt ggcaccattg aacatcgcgt caaggggctg gagttggggg cggatgacta
    40621 tctggtgaag ccgttcgctt ttgctgaact gctggcgcgg gtgcgcactc tcctgcggcg
    40681 cggggcggcg gtgattatcg aaagtcagtt tcaggttgcc gatctgatgg tcgatctcgt
    40741 cagccgcaaa gtcacccgca gcggcacgcg catcactctg accagtaaag agtttactct
    40801 gctggagttt ttcctccgcc atcagggcga agtgctgccc cgctcgctta tcgcctcgca
    40861 ggtgtgggac atgaatttcg acagcgacac taacgccatt gatgtagcgg tgaagcggct
    40921 gcgcggcaaa atcgacaacg actttgagcc aaagctaatt cagaccgtgc gcggcgtggg
    40981 ttacatgctt gaggtgccgg atggtcagta agccatttca gcgcccgttt tcgctggcaa
    41041 cccgcctgac cttttttatc agcctggcca ccatcgcagc gtttttcgcc tttgcatgga
    41101 tcatgatcca ctcagtaaaa gtgcattttg ccgagcagga tattaatgat ttaaaagaaa
    41161 ttagcgccac gcttgaacgg gtacttaatc atcctgacga gacgcaagcc cgacgcttaa
    41221 tgacgctgga agatatcgtc agtggttatt ccaacgtgtt gatctccctg gcagatagcc
    41281 atggtaaaac ggtgtatcac tcccccggtg cgccggatat ccgcgagttt acgcgtgacg
    41341 ccatacccga taaagacgcg cggggcggcg aggtgtatct cctttccggc ccgacgatta
    41401 tgatgccagg gcatggtcac gggcatatgg aacacagcaa ctggcggatg attaacttgt
    41461 cggttggccc gttggtggac ggcaaaccga tttatacgct ctacatcgcg ctttcgatcg
    41521 attttcatct tcattacata aatgatttga tgaataaact tattatgacc gcatcgatca
    41581 tcagcatcct gatcgtcttt atcgtgctat tggcggtaca taaaggtcac gcgccgatcc
    41641 gcagcgtcag ccgtcaaatc cagaatatta cctcgaaaga tctcgacgtt cgccttgacc
    41701 cgcagaccgt gcccattgag ctggaacagc tggtactgtc gttcaaccat atgatcgagc
    41761 gtattgagga tgtctttacc cgccagtcca atttctcagc ggatatcgct cacgaaattc
    41821 gcacgccgat taccaatctc atcacgcaaa cggaaatcgc cctcagccag tctcgcagcc
    41881 agaaggagct ggaagatgtg ctctactcta atctcgaaga gctgacgcga atggcgaaaa
    41941 tggtcagcga tatgctgttt ctcgctcagg ccgataacaa ccagctaatc cccgaaaaga
    42001 aaatgctcaa cctggcggat gaagtcggca aagtgttcga ttttttcgag gcgttagcgg
    42061 aagatcgcgg cgtggagttg cgatttgttg gcgacgaatg tcaggttgcg ggcgatccgc
    42121 tgatgctgcg tcgggcgtta agcaacctgc tttctaatgc cctgcgttat acgccgcccg
    42181 gagaggcaat tgtagtgcgc tgccagacgg tcgatcacca ggtgcaagtt accgtcgaaa
    42241 accccggtac gcccattgcg cccgagcact taccgcgatt gtttgaccgt ttctaccgcg
    42301 ttgacccttc ccgccagcga aaaggcgaag gtagcggcat tgggctggcg atagtgaaat
    42361 cgattgttgt cgcgcataaa ggcacggttg cagtaacgtc agatgcgcgg gggacaaggt
    42421 ttgtgattat gctgccggag agagagtgat tattaagttc aaattcaggg cgtggtgcat
    42481 cacctcctgg tattgttgat caacaaatta gagaaaaact tattaaacat atgaaatatc
    42541 gaaggctttt atttttcaaa gtttcacgag atgtttgtat atgaaactta gttaaatctg
    42601 tgtttcttcc aattgtagat cacaatgccc tttctaaatt ttcat
//
LOCUS       DAZFXT010000028        42345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.028, whole genome shotgun sequence.
ACCESSION   DAZFXT010000028 DAZFXT010000000
VERSION     DAZFXT010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.028"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(41..274)
                     /locus_tag="ACHKW0_001623"
     CDS             complement(41..274)
                     /locus_tag="ACHKW0_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474477.1"
                     /translation="MRDIYHETIYRAFLALSHSENMLEILRIWHETLGDNECDKQKSR
                     IVTALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(276..497)
                     /locus_tag="ACHKW0_001624"
     CDS             complement(276..497)
                     /locus_tag="ACHKW0_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474478.1"
                     /translation="MRDDRFNSLKHEFSGVSDDAGDALSSISKLIRASFFLIGTKEYK
                     STGIDVLNIAADYADFVTEVILRKTTDGD"
     gene            complement(490..714)
                     /locus_tag="ACHKW0_001625"
     CDS             complement(490..714)
                     /locus_tag="ACHKW0_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474479.1"
                     /translation="MLQNKTTPDAAAAALTTLMHALIDIECTAVLAQKEEREEYTLFA
                     LECIRYTATRSMNDAKNILIADCENGGGYA"
     gene            complement(912..1376)
                     /locus_tag="ACHKW0_001626"
                     /pseudo
     CDS             complement(912..1376)
                     /locus_tag="ACHKW0_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536911.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            complement(1369..1566)
                     /locus_tag="ACHKW0_001627"
     CDS             complement(1369..1566)
                     /locus_tag="ACHKW0_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO7474480.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGSHHA"
     gene            1756..2061
                     /locus_tag="ACHKW0_001628"
     CDS             1756..2061
                     /locus_tag="ACHKW0_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7474481.1"
                     /translation="MATANKNAKSQLTTVRVPLDVMQGMESVKLDGESNAGFIVTAMR
                     GEIARRQAEGSGENPLVSSLDALAKVEQIGIKAAEDIGQLVTVAREELQRRKTQESE"
     gene            2186..2515
                     /locus_tag="ACHKW0_001629"
     CDS             2186..2515
                     /locus_tag="ACHKW0_001629"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474482.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKLRSGLSVQKGGSISGPLFLCLKNSNFVVFQFHQGERIPAHIRA
                     YIQSSWL"
     gene            complement(2565..2753)
                     /locus_tag="ACHKW0_001630"
     CDS             complement(2565..2753)
                     /locus_tag="ACHKW0_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7474483.1"
                     /translation="MLSTDRFIREKECEKLTGLSRTCRYRMEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(3118..4347)
                     /locus_tag="ACHKW0_001631"
     CDS             complement(3118..4347)
                     /locus_tag="ACHKW0_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7474484.1"
                     /translation="MSRAMNKLSDTLLRKLHGKPAEKNTFYSDGGNLSVKYLTSGKLT
                     WYFTYRAGTGRETRPERIKLGSYPDLSLKAAREKAAQCRAWLAEGKNPRHEMNYTVQE
                     ALKPVTVGDALTYWLESYVKENRVDYIALKRRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFVLQACKQALKFCRRRRYAISNVLDDLNVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYVALIRLLIVFGARTVELRLSEIGEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGEVKQEASVSQYGRLAHRRLKHPQWSL
                     HDIRRTFTTMLNDLGADPHVVEQLTGHQMPGMQRVYNHSRYLDAKRDALDMWTERLGI
                     LAGAHENVTTLPVARRK"
     gene            4335..4517
                     /locus_tag="ACHKW0_001632"
     CDS             4335..4517
                     /locus_tag="ACHKW0_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001332680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474485.1"
                     /translation="MLCSRFTPSLRKSDYTSDYTDRYTKGYNVKQAGTRKNTNLLIIK
                     NIMHNLKHYETAKNTK"
     gene            4524..5429
                     /gene="tqsA"
                     /locus_tag="ACHKW0_001633"
                     /pseudo
     CDS             4524..5429
                     /gene="tqsA"
                     /locus_tag="ACHKW0_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
     gene            complement(5454..6842)
                     /gene="pntB"
                     /locus_tag="ACHKW0_001634"
     CDS             complement(5454..6842)
                     /gene="pntB"
                     /locus_tag="ACHKW0_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HFO7474486.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(6853..8385)
                     /gene="pntA"
                     /locus_tag="ACHKW0_001635"
     CDS             complement(6853..8385)
                     /gene="pntA"
                     /locus_tag="ACHKW0_001635"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HFO7474487.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKTAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            8909..9853
                     /gene="ydgH"
                     /locus_tag="ACHKW0_001636"
     CDS             8909..9853
                     /gene="ydgH"
                     /locus_tag="ACHKW0_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protein YdgH"
                     /protein_id="HFO7474488.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            10039..11421
                     /gene="ydgI"
                     /locus_tag="ACHKW0_001637"
     CDS             10039..11421
                     /gene="ydgI"
                     /locus_tag="ACHKW0_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7474489.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            11458..12180
                     /gene="folM"
                     /locus_tag="ACHKW0_001638"
     CDS             11458..12180
                     /gene="folM"
                     /locus_tag="ACHKW0_001638"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HFO7474490.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(12177..12512)
                     /gene="ydgC"
                     /locus_tag="ACHKW0_001639"
     CDS             complement(12177..12512)
                     /gene="ydgC"
                     /locus_tag="ACHKW0_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HFO7474491.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            12641..13360
                     /gene="rstA"
                     /locus_tag="ACHKW0_001640"
     CDS             12641..13360
                     /gene="rstA"
                     /locus_tag="ACHKW0_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HFO7474492.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            13364..14669
                     /gene="rstB"
                     /locus_tag="ACHKW0_001641"
                     /pseudo
     CDS             13364..14669
                     /gene="rstB"
                     /locus_tag="ACHKW0_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
     gene            14745..15674
                     /gene="tus"
                     /locus_tag="ACHKW0_001642"
     CDS             14745..15674
                     /gene="tus"
                     /locus_tag="ACHKW0_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006274 - DNA replication termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HFO7474493.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(15671..17074)
                     /gene="fumC"
                     /locus_tag="ACHKW0_001643"
     CDS             complement(15671..17074)
                     /gene="fumC"
                     /locus_tag="ACHKW0_001643"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HFO7474494.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(17217..18863)
                     /gene="fumB"
                     /locus_tag="ACHKW0_001644"
     CDS             complement(17217..18863)
                     /gene="fumB"
                     /locus_tag="ACHKW0_001644"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7474495.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            19062..20237
                     /gene="manA"
                     /locus_tag="ACHKW0_001645"
     CDS             19062..20237
                     /gene="manA"
                     /locus_tag="ACHKW0_001645"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009298 - GDP-mannose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HFO7474496.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20338..21846
                     /gene="ydgA"
                     /locus_tag="ACHKW0_001646"
     CDS             20338..21846
                     /gene="ydgA"
                     /locus_tag="ACHKW0_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7474497.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLESMEINGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMLALNVPVVPAIPQQ"
     gene            complement(21891..23156)
                     /gene="uidC"
                     /locus_tag="ACHKW0_001647"
     CDS             complement(21891..23156)
                     /gene="uidC"
                     /locus_tag="ACHKW0_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HFO7474498.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLIFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(23195..24568)
                     /gene="uidB"
                     /locus_tag="ACHKW0_001648"
     CDS             complement(23195..24568)
                     /gene="uidB"
                     /locus_tag="ACHKW0_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HFO7474499.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSFATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(24565..26376)
                     /gene="uidA"
                     /locus_tag="ACHKW0_001649"
     CDS             complement(24565..26376)
                     /gene="uidA"
                     /locus_tag="ACHKW0_001649"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HFO7474500.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(26764..27354)
                     /gene="uidR"
                     /locus_tag="ACHKW0_001650"
     CDS             complement(26764..27354)
                     /gene="uidR"
                     /locus_tag="ACHKW0_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HFO7474501.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(27583..28350)
                     /gene="hdhA"
                     /locus_tag="ACHKW0_001651"
     CDS             complement(27583..28350)
                     /gene="hdhA"
                     /locus_tag="ACHKW0_001651"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HFO7474502.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            complement(28462..29490)
                     /gene="malI"
                     /locus_tag="ACHKW0_001652"
     CDS             complement(28462..29490)
                     /gene="malI"
                     /locus_tag="ACHKW0_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HFO7474503.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            29665..31257
                     /gene="malX"
                     /locus_tag="ACHKW0_001653"
     CDS             29665..31257
                     /gene="malX"
                     /locus_tag="ACHKW0_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HFO7474504.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            31267..32439
                     /gene="malY"
                     /locus_tag="ACHKW0_001654"
     CDS             31267..32439
                     /gene="malY"
                     /locus_tag="ACHKW0_001654"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HFO7474505.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NMARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            32543..33544
                     /gene="add"
                     /locus_tag="ACHKW0_001655"
     CDS             32543..33544
                     /gene="add"
                     /locus_tag="ACHKW0_001655"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HFO7474506.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(33579..34619)
                     /gene="ydgJ"
                     /locus_tag="ACHKW0_001656"
     CDS             complement(33579..34619)
                     /gene="ydgJ"
                     /locus_tag="ACHKW0_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HFO7474507.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            34862..34987
                     /gene="blr"
                     /locus_tag="ACHKW0_001657"
     CDS             34862..34987
                     /gene="blr"
                     /locus_tag="ACHKW0_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HFO7474508.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            35260..35475
                     /gene="ydgT"
                     /locus_tag="ACHKW0_001658"
     CDS             35260..35475
                     /gene="ydgT"
                     /locus_tag="ACHKW0_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HFO7474509.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            35561..36001
                     /gene="ydgK"
                     /locus_tag="ACHKW0_001659"
     CDS             35561..36001
                     /gene="ydgK"
                     /locus_tag="ACHKW0_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HFO7474510.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            36078..36659
                     /gene="rsxA"
                     /locus_tag="ACHKW0_001660"
     CDS             36078..36659
                     /gene="rsxA"
                     /locus_tag="ACHKW0_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HFO7474511.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            36659..37237
                     /gene="rsxB"
                     /locus_tag="ACHKW0_001661"
     CDS             36659..37237
                     /gene="rsxB"
                     /locus_tag="ACHKW0_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HFO7474512.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            37230..39260
                     /gene="rsxC"
                     /locus_tag="ACHKW0_001662"
     CDS             37230..39260
                     /gene="rsxC"
                     /locus_tag="ACHKW0_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HFO7474513.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQQANAEPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAAVE
                     AAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
     gene            39261..40319
                     /gene="rsxD"
                     /locus_tag="ACHKW0_001663"
     CDS             39261..40319
                     /gene="rsxD"
                     /locus_tag="ACHKW0_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HFO7474514.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            40323..40943
                     /gene="rsxG"
                     /locus_tag="ACHKW0_001664"
     CDS             40323..40943
                     /gene="rsxG"
                     /locus_tag="ACHKW0_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HFO7474515.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            40947..41642
                     /gene="rsxE"
                     /locus_tag="ACHKW0_001665"
     CDS             40947..41642
                     /gene="rsxE"
                     /locus_tag="ACHKW0_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HFO7474516.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            41642..42277
                     /gene="nth"
                     /locus_tag="ACHKW0_001666"
     CDS             41642..42277
                     /gene="nth"
                     /locus_tag="ACHKW0_001666"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HFO7474517.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
BASE COUNT        10291 a        10934 c        10357 g        10763 t
ORIGIN      
        1 ccgctggcag ttaagccagg atatttaagt ttcattattt ttattctccg gtgtgctgtt
       61 ctttatatct gtcgtgcaat aggtctattt cttgcagttc cattattaca ggctcaagaa
      121 gcgttattaa tgccgtaaca attcttgatt tttgtttgtc gcattcattg tcgccaagtg
      181 tttcatgcca tatgcgcaat atttccaaca tattttcact gtgagaaagt gcaagaaatg
      241 cgcggtatat tgtttcgtgg taaatatcac gcatattaat ccccgtccgt cgtttttctt
      301 aaaataacct ctgtcacgaa atcagcataa tcggcggcga tattcagtac gtctattcca
      361 gttgatttat attctttcgt gccaattaag aaaaaagatg ctctaatcag tttggatatt
      421 gacgaaagcg catcacccgc atcatcagat acgccggaaa attcatgttt cagggaatta
      481 aaacgatcat cacgcataac cccccccatt ttcacaatca gcaataagaa tatttttagc
      541 gtcattcatc gaccgcgtgg cggtgtatcg gatacattcc agggcgaaca atgtgtattc
      601 ttcccgttct tccttttgtg caagcactgc tgtgcattca atatcaataa gtgcgtgcat
      661 cagcgtagtg agcgcggcgg ctgctgcgtc cggtgtggtt ttattctgca acatatctta
      721 actccactga acttacgtat ttacacgggt ttccttttac ctgtcgcaca cggtaggaat
      781 cgtttttaat cacttccgct atttgcccaa acttttttac cgctgtacgg cggctcttgt
      841 agcggtaagt gtttatctcc cttctgctca ttaccgcttc attgtgatat ctggttatca
      901 ctatcagcac ttcaaacatg acgcaccccc tgacgaatac gggcgacaaa aatcagatgt
      961 gcgcgcggca actgggcgcg ggcttcgcgt tcagtggctg cggtaacggt gtaaatatca
     1021 atgctgctgt ggtggcactg cataaagcgc cagacaaagt aagggcgtgc ggatacagcc
     1081 atgtgaaagg ctcccaattt aatgttaagg ggtctcacca ctacgctgct aaacggggtg
     1141 gcgagacgta acagggttag cagactggca aattgggaaa ccagcgagcg caaaggctcc
     1201 cccgttacgc cccgccataa tgcgggtatg ggtaggttta cggacacaaa aaaaccgctt
     1261 atcggatatg ggcggctgtc cgccaattta ttcaggctgc taaacccggt cgccatgtgg
     1321 gcgacggggg aagcatacag ccccgtgata attttttgca agcggttttt acgcatgatg
     1381 tgacccctga cgaatacgag cggcaaatac agcaacacaa cctgacgggc aacggctacg
     1441 cgcttcgcgt tccgtccagg cggttacgtg gatgatttga gattctccgg cactcagggc
     1501 cagaaaacgc cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc
     1561 aaccataacg gctcctagtt tacgttgttg gttagaagcc ccgttactgc tcctacagtg
     1621 cggggctttg ctctttgtat ttcaacaatc cttttggtgt gtttcatgtt atggacacat
     1681 gaaacacacg tcaaggcttt ttgtatttct ttttttgtgt atactgaaac acaccgatga
     1741 ttaggagttt cagaaatggc aacggctaac aaaaacgcaa aatcacaact gacaaccgtc
     1801 agagtcccac tagatgttat gcaagggatg gaatccgtta agctggacgg cgaaagcaat
     1861 gccggattta tcgtaaccgc catgcgcggt gaaatcgccc gccgccaggc agaaggaagc
     1921 ggagaaaatc cccttgtgtc ttcactggat gctttggcta aggtcgaaca aattggcatc
     1981 aaggcagcgg aggatatcgg gcagcttgtc accgttgcac gcgaagaact ccagcgtcgc
     2041 aagacccaag aatcagaata atcactatca gcgccgtggc gtgaggtact acggcgcatt
     2101 gctttacagg cgcacacaat gaccaacgaa gaatcaaccg aaaaaacatc acctgcacga
     2161 aaaagacgac gcagaaagaa agagcatgaa tcagaacgat tcgcgccgtg ttcttttgcc
     2221 cttgagaagt accttaaaga atactcatca aaggagagag cttcgcagtt atggcaacgc
     2281 actgaaccag actgattgca ttgcacacca gcctgatagc ggctatcatc cccgtgctta
     2341 tgtttgggat cacacacaca aactgcgcag cgggttatct gttcagaaag gcggctccat
     2401 ttcggggccg ctttttttat gcctgaaaaa ctccaatttt gttgtttttc agtttcacca
     2461 gggcgaacga atccctgccc acattcgggc gtatattcaa tcttcatggt tatagctctg
     2521 tgttcagatg attgatgtgt ggcggctgcg tgccgccagc gtgattaatg aactgccttg
     2581 cagctatcct tccaggccag aacctcggat aaagaccagc caacggaacg accgccaagt
     2641 ttacgacgtg atgggaattg tccggccttt tccatgcggt agcgacacgt acggctaagg
     2701 ccggttagtt tttcgcattc tttttcacgt ataaaccgat cagtgcttaa cactattgcc
     2761 ccctttcgtt tcttaaagag tcatcaggtg tcttattgtg tcgtattgtt cccgctagag
     2821 tgatgaatgg caaatgttgg tggcgcatgg tttacagaaa gaggaataat caggataaaa
     2881 tcatttaaat tcatgttaat acaaaggcat aaaatctcgt tttatgcctt ttttctcacg
     2941 ctttaaagcg tgattcgcta atgtataaaa aaccagtaat ataataaaaa tcagcaactt
     3001 ataaatctgt atgctttttc gcctcttgtt cgtgattgtt ccacgttgtt gcacattgtt
     3061 tcacgttgta tctgttcgca tatccagtat gcgcatactg aaaaaacccg aaaaaaatta
     3121 ttttcttctg gctacgggta gcgtggttac gttttcatgt gctcccgcca gtatccctaa
     3181 ccgctccgtc cacatatcca gcgcatcacg tttcgcatcc agataacggg aatggttata
     3241 gactcgttgc atccctggca tctgatgacc tgtaagctgc tccacgacgt gaggatccgc
     3301 gcctaaatcg ttcagcatcg ttgtaaaggt gcgccggatg tcatgcagtg accattgagg
     3361 gtgttttagc ctcctgtgcg ctaatctgcc gtactgcgat acgctggcct cctgtttcac
     3421 ttcccccagc aataagcccg tgtgcctgtt ctgctccacc agcttcatga caaacggcag
     3481 gatcgcttcc ggtatgggcc ggaatattgc gaccttcgtt ttgctgtgct ccttcggaac
     3541 ggtccatagc atttccgtaa aatcccactc cccgatctcc gataacctca gttctaccgt
     3601 tctggcaccg aagacaatca ggaggcggat taacgcgacg tagtaagggg aaaatatttt
     3661 tttatccagt gcctgcaata actcgccaag ttctttggtg cttaagacac gctcgcttat
     3721 atccggtttt ttcccaacgt ccgcaacgtt cagatcgtcc agaacgttgc tgattgcata
     3781 gcgccgccta cggcagaact taagcgcctg tttgcacgcc tgtagcacga atccggcagt
     3841 aacaggcgtt cgctttgcca cctggtcaaa acaagccagc cagtgccgta gctcgcattt
     3901 atccagcggc atagcaccaa tgtgctgtat tacgtgatta ttaagtcgcc ttttcagggc
     3961 gatataatcc acgcggtttt cctttacgta agactcaagc cagtaggtaa gcgcatcgcc
     4021 aaccgttacg ggctttaacg cttcctgtac ggtgtaattc atctcatgac gtggattttt
     4081 cccctcagcc agccatgcgc gacactgtgc ggctttttcc ctggctgctt tcaggctcag
     4141 atcaggataa cttcccagct taatgcgttc gggtcgtgtc tcccttcccg ttccggccct
     4201 gtatgtgaaa taccatgtta atttccctga tgttaaatat ttcacgctca ggtttccgcc
     4261 atcactataa aacgtgtttt tctcagctgg cttaccatga agtttcctta gcaatgtatc
     4321 gctcagtttg ttcattgctc tgctcacgtt ttaccccctc tttacgaaaa agtgactaca
     4381 ccagtgacta caccgatcgt tacacaaagg gctacaacgt gaaacaagct ggaacaagga
     4441 aaaacacaaa tctgttgata atcaaaaaca taatgcacaa cctgaaacat tatgaaacag
     4501 caaaaaacac taaatgataa aatgctgtta ttcttaaccc gctggtgcaa cacatggtcc
     4561 gctggcgagt gccgcgtgta ctggcggtgt cgattttgat gaccatcatc gtgatggcga
     4621 tggtgttgct attagcttat ctgggttccg cgctcaacga gttgacgcgg acgttaccgc
     4681 aatatcgcaa ctctattatg acgccgctgc aagctcttga gccgttgttg caacgcgtag
     4741 ggattgacgt ctcagttgac cagctggcgc attacattga tccgaacgcg gcgatgacgt
     4801 tgctcaccaa cttattgacg cagttatcta atgccatgtc atcaatattt ttattgctgc
     4861 tgacggtgct gtttatgctg ctcgaagtgc cacaattgcc cggaaaattt cagcaaatga
     4921 tggcgcgtcc ggttgaaggg atggcggcga ttcaacgtgc aattgacagt gtttctcatt
     4981 atctggtgct gaaaacagcc atcagcatca tcaccggcct ggtcgcctgg gcgatgctcg
     5041 ccgcactcga tgttcgcttc gcttttgtct ggggattgat ggcctttgcg tttaattaca
     5101 tccctaatat tggttcagtt ctcgcggcaa tcccccctat cgctcaggta ctggtgttta
     5161 atggcttcta cgaagcgttg ctggtgctgg cgggatatct gctgattaat ctggtcttcg
     5221 gcaatattct ggagccgcgc atcatgggac gtgggctggg gctttccaca ttggtggtat
     5281 ttttgtcgtt gattttttgg ggatggttgt taggaccggt gggtatgctg ctttccgtgc
     5341 cgttaacaat tattgtcaaa attgcgcttg aacaaacagc gggaggtcaa agcatcgccg
     5401 ttctgttaag cgatctcaat aaagagtgac ggcctccgca gaggccgtca gggttacaga
     5461 gctttcagga ttgcatccac gctggctttg gcgtcaccaa acagcatgtg ggtgttttcc
     5521 ttgaagaaca gcgggttttg cacaccagca tagccagtgt tcatcgaacg tttaaagaca
     5581 atcacgttct gcgctttcca cacttccagc acaggcatac cagcaatcgg actcttcgga
     5641 tcatcctgcg ccgccgggtt aaccgtatcg ttagcaccaa tcaccagtac ggtatcggta
     5701 tcagcaaagt catcgttgat ctcgtccatt tccagcacga tgtcatacgg tacttttgct
     5761 tcagccagca atacgttcat atgtccaggc aaacgccccg caaccgggtg gataccgaaa
     5821 cgcacgttga taccacgagc gcggagtttc tcggtaattt cagcgacagg atattgcgcc
     5881 tgcgcgactg ccatgccgta ccccggagta atgatcactg aatgggagtt tttcagcagt
     5941 tccgctgtct cttctgcggt gatttcgcgg tgctcaccca cttcctgatc atcgccagta
     6001 gaagagccgt cggtgccgaa accacccgca ataacgctga taaaggaacg gttcatcgcc
     6061 ttacacataa tgtaagaaag gatagccccc gaagaaccga ccagcgcacc ggtcacaatc
     6121 agcaggtcgt tgctgagcat aaagcccgca gccgcagccg cccagccgga gtacgagttc
     6181 agcatcgaca ccaccactgg catatctgca ccaccgatgg aggcgactaa atgccagccg
     6241 aataccagcg caatggcggt cattatcagc aatgccagca cttgcaggcc gacgctgtcc
     6301 gtgcgaacaa atacaatcag cagcaggaag gaaacgacca gagccgccag gttcattttg
     6361 tgacggtttg gcagcatcaa cggtttagac gaaatcttgc cacacagttt gccgaacgcc
     6421 accaccgaac ccgtgaacgt taccgccccg atgaagatac cgaggaacac ttccgtcagg
     6481 tgaatattga ccagaatcgg tgccattccc gcgtcatgat gcagataact gttaaagcca
     6541 accagcactg ccgccagacc cacgaagcta tgcaggatcg ccaccagttc tggcatttcg
     6601 gtcatttcaa ctttcttcgc cagacggata ccaattgccc caccaatgac catcgccagc
     6661 aagatccagc caacattacc cgtatccggt ccaaaaatgg ttgcgattaa cgcaatcgcc
     6721 atcccggcga taccgaagtt gttaccctgg cgagacgttt catgtttcga aagaccggcc
     6781 agactgaaga taaacaggat cgcggcaaca atgtatgcag ctgtaactaa tcctccagac
     6841 atatgttacc ccttaatttt tgcggaacat tttcagcatg cgctgagtca cggtgaagcc
     6901 accgaaaata ttaatgctgg ctataagcac cgcgataaaa ctaaggaagc taacccagcc
     6961 gccctggcca atctgcaaca gtgctccgac aacaataatc cctgaaatcg cgttggtgac
     7021 cgacatcaac ggtgtatgca gcgcatgcga tacattccac accacgtaat aaccgacaac
     7081 gcaggccagc gcgaaaacgg tgaagtgccc aagaaactct ttcggcgcaa cgcttgccag
     7141 ccaaccgaaa aggatgattg ccagcgccat caatgcgtat ttacgccacg gtgagcaggc
     7201 acatttttcc tcagttttca cttccggtgc cgctttttgt gccgcctgcg gctgagctga
     7261 tacctgaatc ggcggtgccg gccaggtaat ttcgcccgca cggatcacgg tcacgccgcg
     7321 aatcaccaca tcatcaaaat caacagtgat attgccgtct ttctctttgc acaacagttt
     7381 cagcagatta acgaggttag taccgtaaag ctgtgaggat tgcgtcggca gacggcccgg
     7441 aagatcggta taaccaatca ctttgacacc attttccgta gtgaagattt cacccggcac
     7501 ggtgtattca cagttgccac cgttttgggc tgccaggtcg acaatcacac tgcccgcctt
     7561 catggagtca accatttcac gggtaattag cttcggcgct gttttgcctg gaataagcgc
     7621 ggtggtgaca atgatatcga cctcttttgc ctgggcggca aagagttcca tttccgcttt
     7681 gatgaacgcg tccgacatca ctttggcata gccatcgccg ctgcccgctt cctctttaaa
     7741 atccagctcg aggaattccg cgcccatact ttgaacttgt tctttcactt ccgggcgggt
     7801 gtcgaatgca cgcacaatcg cgccgagact gtttgctgcg ccaatggcgg ccagacctgc
     7861 aacacccgca ccaatcacca tcacttttgc cggtggcact ttcccggccg cagtaatttg
     7921 cccggtaaag aagcgcccaa attcatgtgc cgcttcaaca atggcgcgat aaccggcgat
     7981 gttcgccatc gagcttagtg cgtccagcga ttgtgcgcgt gagatacgcg gcacagaatc
     8041 catcgccatc acggtcacgt tacgttccgc aagtttttgc attaattccg gattctgcgc
     8101 aggccagata aaactcacca gcgttgtccc aggattcagt aacgcaattt catcatctaa
     8161 cggcgcattg accttcagaa tgatctctga ctgccagacg ctattccctt ctacaatttc
     8221 agcgcccgct tgcacaaacg ctttatcgtc aaaacttgcc agttgacccg cgccgctctc
     8281 taccgcgacg gtaaaaccca gtttcagcag ctgttccact gtttttggcg ttgctgcaac
     8341 acgggtttca ttggttaacc gttctcttgg tatgccaatt cgcatgatat tcccttccat
     8401 cggttttatt gatgatggtt tgcctgtgtc aggagccaca caagccgctc atgtacgagc
     8461 taaatgttac tccgttaaaa taaattagta acaaacgccc tataacgtac tgaaaattat
     8521 gcctgtgatc tagcgccaaa aatcagtatt tcggcgtgaa ctcgcaaaat attaacgatt
     8581 cagccgtgat agtgggataa acaccttaga acgccggata aagactgata attgtcttcg
     8641 acggtcgggt aaaacgagac atcgccccgg cacgaatcac tacttaacat taaattaact
     8701 tatataattc agttgcttca gtagtaatga tgctgatacg gctgtttttt aagcatagac
     8761 ggtcatttga gcaggattaa aattggctta aagaatgtga tatgaaaaat gacgcagaca
     8821 gttacaccgt ttaaatgcaa taatcagcca cgtttctcgt taataacaat accagtacct
     8881 ggtttgcgca aggcgaagga ttatttttat gaagcttaag aacaccctcc tggcgtcggc
     8941 actgctttct gctacggcat tctccgttaa cgcagcaaca gaactgacac cggagcaagc
     9001 ggcagcggtt aaaccttttg accgtgtagt ggttaccggt cgttttaatg ctattggcga
     9061 agcggtgaaa gccgtttctc gtcgcgcaga taaagaaggt gccgcctctt tttatgttgt
     9121 cgacacttct gattttggta acagcggtaa ctggcgtgtg gtcgctgacc tctataaagc
     9181 cgatgctgaa aaagcagaag aaacaagtaa tcgcgtaatt aacggtgttg tcgaactgcc
     9241 gaaagatcag gctgttctga ttgaaccgtt tgacacggtc accgtccagg gcttctatcg
     9301 tagccagcca gaagtcaatg atgccatcac caaagcggca aaagcgaaag gtgcctactc
     9361 tttctacatc gttcgtcaaa tcgatgccaa ccagggcggc aaccagcgta ttactgcatt
     9421 catctataaa aaagatgcta agaaacgtat cgtccagagc ccggatgtga tcccggcaga
     9481 ttccgaagca ggacgtgcag ctctggctgc cggtggcgaa gccgcgaaga aagttgagat
     9541 cccaggtgtt gcgactaccg catcaccaag ttctgaagtc ggtcgtttct ttgaaaccca
     9601 gtcatcaaaa ggcgggcgtt acaccgtcac gctcccggat ggcactaaag tcgaagaact
     9661 gaacaaagcg accgcagcga tgatggtccc gttcgacagc atcaaattct ctggcaacta
     9721 cggcaacatg accgaagtct cctatcaggt tgcgaaacgt gccgcgaaga aaggtgctaa
     9781 gtattaccac atcacccgcc agtggcagga acgtggtaat aacctgaccg tcagcgcaga
     9841 tctgtataaa taactattca gttactccta taaggcggct tgatgccgcc tttcgcattt
     9901 ttagtcacaa atttccccgc gaccattgca tgcagacaca tctcttcgta aaatcccgcg
     9961 cttcagtggc aaactccatt tttatgcgtt ttgccctaat atttattctt cttatcacgt
    10021 tttaatcact ggatatcgat ggaaaagaaa ctgggactga gcgcactcac cgcgctggta
    10081 ttaagctcaa tgctgggcgc gggtgttttc agtctgccgc aaaatatggc ggcagttgcc
    10141 agcccggcag cactgctcat cggctggggt attactggcg ctggcatttt attgctggcc
    10201 tttgccatgc tgatcctcac gcgcattcgt cccgaacttg acggcggtat ctttacctat
    10261 gcccgcgaag ggtttggcga gctaatcggc ttttgttccg catggggata ctggctgtgc
    10321 gcagtcatcg ccaacgtctc ctatctggtg atcgtttttt ccgcgttaag cttttttacg
    10381 gacacgccgg aattgcgcct gttcggcgat ggtaatacct ggcaatcgat agtcggtgca
    10441 tcggctttat tgtggattgt tcactttttg atcctgcgcg gggtgcaaac cgctgccagc
    10501 attaacctgg tggcgacact ggcaaaattg ttgccgctgg gtctgtttgt tgtgctggcg
    10561 atgatgatgt tcaaactgga taccttcaag ctcgacttca ccggacttgc acttggcgta
    10621 cccgtgtggg aacaggtgaa aaacaccatg ctgatcaccc tgtgggtttt cattggtgtg
    10681 gaaggtgccg tggttgtttc tgcgcgtgcg cgtaataaac gtgatgttgg caaagcgaca
    10741 ctgctggcgg ttctctccgc tctgggcgtt tacttgctgg taacgctgct ttcgctgggc
    10801 gtggtggcac gtcctgaact ggctgaaatt cgtaacccgt caatggccgg tctgatggtg
    10861 gaaatgatgg gaccatgggg cgaaatcatc atcgctgccg gtttgatcgt ttccgtttgc
    10921 ggcgcgtact taagctggac catcatggcg gcagaagttc cgttcctggc tgccactcat
    10981 aaagcattcc cacgcatttt tgcgcgccag aacgcacaag ctgcgccatc cgcctcgctg
    11041 tggttgacca acatctgcgt gcaaatttgt ctggtactca tctggctgac cggttccgat
    11101 tacaacacgt tgctgaccat cgcctcagaa atgattctgg tgccctattt cctggtcggc
    11161 gcgttcttgc tgaaaatcgc aacccgtcca ctgcataaag cggtaggtgt cggtgcctgc
    11221 atttatggct tatggttatt gtacgcctct ggtccaatgc acttgctgtt atccgttgtt
    11281 ctgtatgcgc cggggctgct ggttttcctg tatgcgcgca aaacgcatac acatgataat
    11341 gtactgaacc gtcaggaaat ggtgctgatt ggcatgctgt taattgcctc agttccggcg
    11401 acctggatgc tggtgggata acgttcatcc catcgttgga tagcaaagga gataacgatg
    11461 ggtaaagccc agcccttgcc aatattaatt actggcggag gtcgtcgcat cggcctcgcc
    11521 ctcgcctggc atttcattaa tcaaaagcaa ccggtgattg tcagctatcg gacgcactat
    11581 ccagccattg atggactgat taaggcaggt gcgcagtgta ttcaggctga tttttcgacc
    11641 aacgacggtg tgatggcgtt tgccgatgaa gtactaaaaa gcacccatgg tctgcgtgct
    11701 attttgcata acgccagtgc gtggatggcg gaaaaaccag gtacaccact ggccgacgta
    11761 ctggcttgca tgatgcagat ccacgttaat accccatacc tgctcaacca tgcgctggaa
    11821 agattactgc gtgggcacgg acacgccgcc agcgatatca ttcactttac cgattatgtg
    11881 gtggagcgcg gtagcgacaa acatgttgcg tatgctgcaa gcaaagcggc actggataat
    11941 atgacccgct cgtttgcccg caagctggca ccggaagtga aagtgaattc tattgcgcca
    12001 tcgctgatcc tgtttaatga acatgatgat gccgaatatc gacaacaggc gctgaataaa
    12061 tcactgatga aaaccgcgcc tggcgagaaa gaagtgatcg acctggtcga ttacttactt
    12121 accagctgct ttgtcaccgg acgcagtttc ccacttgatg gcggtcgtca tctgcgttaa
    12181 tgcagcttta tccagcatat aatcaacacc catgcgctta tcccccaaca cgcaacagaa
    12241 ccaacaaaag cggcgggcaa gcgcatcatt ccggtgaaat accacagcga caccaggtaa
    12301 acaaaatagg gaataatcga ccacatacta aaaatgatgg ttgcgcgtaa ggcttcaatg
    12361 ccgcgttcgc tggcaacaat ataatgcgcg ataagcgcaa aggtcgggaa aagtggaatc
    12421 agcccggcga tataataatt tttcgttttt gctaaaacac caatcaacag cactaccagc
    12481 gcaccgagcg cggctttgat taccagcccc atctttttac cttaacactt ccataacaag
    12541 tcatcagtag aatacctgat gaaaacttgt ttagaaacga ttgatagtaa gtaaaaacag
    12601 cgcggtgtat tgtgacgttt ttatatctac cgtgaatgtt atgaacacta tcgtatttgt
    12661 ggaagatgat gcggaagtcg gttcactgat tgccgcgtac ctggcaaaac atgatatgca
    12721 ggttaccgta gaaccgcgcg gcgaccaggc cgaagaaacc attttgcgag aaagtccgga
    12781 tttggtgtta ctcgacatca tgctaccagg caaggacggc atgaccattt gtcgtgattt
    12841 acgcgcaaag tggtctggac cgattgttct tctaacctct ctcgatagcg atatgaacca
    12901 catcctggca ctggaaatgg gtgcctgcga ctatattctc aaaacgacgc cccctgctgt
    12961 tttgctagcg cgtttacgtt tgcatttgcg tcagaatgag caagccacac tgaccaaagg
    13021 tcttcaggaa acgtctctga ctccctacaa agccctgcat ttcggcacgt tgaccatcga
    13081 tcccatcaac cgcgtagtca ccctggctaa cactgaaatc tcgctctcga cagctgattt
    13141 cgaattattg tgggaattag ctacccatgc cgggcaaatc atggaccgcg atgcattgct
    13201 gaaaaattta cgcggcgtca gttatgacgg actggatcgt agcgtggacg tggctatttc
    13261 gcggttaaga aaaaaactgc tcgataacgc cgcagaacct tatcgcatta aaactgtgcg
    13321 taacaaaggc tatctttttg cgcctcatgc atgggaataa gcgatgaaaa aactgtttat
    13381 ccagttttac ctgttattgt ttgtctgctt ccttgtgatg tctctgctgg ttgggctggt
    13441 gtacaaattt accgccgaac gcgcgggcaa acagtcgctg gatgatttga tgaacagttc
    13501 gctgtatctg atgcgcagcg aattgcgaga gatcgatccc cccacacgac tggggtaaga
    13561 cgctgaaaga gatggattta aatctctctt tcgatctgcg tgtcgagcca ctgagtaaat
    13621 accatcttga tgatatttcc atgcaccgac tgcgtggcgg cgaaattgtc gccctggacg
    13681 atcagtacac gtttttgcag cgtatcccgc gcagccacta cgtgctggca gttggtcctg
    13741 ttccttatct ttattatctc catcagatgc gattgctgga tatcgccctg atcactttta
    13801 ttgctatttc cctcgccttt ccggtgttta tctggatgcg tccgcactgg caggatatgt
    13861 taaaactgga agcagcggcg caacgatttg gcgatgggca tctcaatgaa cgtatccact
    13921 ttgatgaggg ttcgagcttt gaacgacttg gtatcgcatt taaccagatg gcggacaata
    13981 tcaacgcctt gattgccagc aaaaaacagc ttattgacgg tatcgctcac gaactgcgaa
    14041 caccgttagt gcgcctgcgt tatcgactgg agatgagcga taacctgagc gccgccgaat
    14101 cccaggcgtt gaatcgtgat atcagtcaac ttgaagcttt aattgaagag ctgctgactt
    14161 atgcccgact cgatcgccca caaaacgagc ttcatcttag cgaaccagac ctgccgttgt
    14221 ggctgtcaac gcatctggca gacattcagg cagtaacgcc cgataaaacg gtacggatta
    14281 aaacgctcgc gcaaggccat tatgcggcgt tggatatgcg cttaatggag cgcgtgctgg
    14341 ataatttgct caataacgcc ctgcggtact gccattcaac ggttgaaacc agcctgctac
    14401 tgtcggggaa tagagcgaca ttaattgttg aggatgatgg cccagggatt gccccagaaa
    14461 accgcgaaca tatctttgaa ccttttgttc gcctcgatcc cagccgggat cgctcaaccg
    14521 gcggctgcgg gctggggctg gcaattgtcc actctatagc actggcaatg ggcggtacgg
    14581 ttaattgtga caccagcgaa ctgggtggtg ctcgcttctc gtttagctgg ccgttatggc
    14641 ataacatccc gcaatttacc tctgcctgac actacgcgca cgatggtcaa gtcaccacca
    14701 ctgtgctata aaataagtat gttgtaacta aagtggttaa tattatggcg cgttacgatc
    14761 tcgtagaccg actcaacact acctttcgcc agatggaaca agagctggct gcatttgccg
    14821 ctcatcttga gcaacacaag ctattggttg cccgcgtgtt ctctttgccg gaggtaaaaa
    14881 aagaggatga gcataatccg cttaatcgta ttgaggtaaa acaacatctc ggcaacgacg
    14941 cgcagtcgct ggcgttgcgt catttccgcc atttatttat tcaacaacag tccgaaaatc
    15001 gcagcagcaa ggccgctgtc cgtctgcctg gcgtgttgtg ttaccaggtc gataaccttt
    15061 cgcaagcagc gttggtcagt catattcagc acatcaataa actcaagacc acgttcgagc
    15121 atatcgtcac ggttgaatca gaactcccca ccgcggcacg ttttgaatgg gtgcatcgtc
    15181 atttgccggg gctgatcacc cttaatgctt accgcacgct caccgttctg cacgaccccg
    15241 ccactttacg ctttggttgg gctaataaac atatcattaa gaatttgcat cgtgatgaag
    15301 tcctggcaca gctggaaaaa agcctgaaat caccacgcag tgtcgcaccg tggacgcgcg
    15361 aggagtggca aagaaaactg gagcgagagt atcaggatat cgctgccctg ccacagaacg
    15421 cgaagttaaa aatcaaacgt ccggtgaagg tgcagccgat tgcccgcgtc tggtacaaag
    15481 gagatcaaaa acaagtgcaa cacgcctgcc ctacaccgct gattgcactg attaatcggg
    15541 ataatggtgc gggcgtgcca gacgttggtg agttgttaaa ttacgatgcc gacaatgtgc
    15601 agcaccgtta taaaccacag gcgcagccgc ttcgtttgat cattccacgg ctgcacctgt
    15661 atgttgcaga ttaacgcccg gctttcatac tgccgaccat ctgttctggc cgtacccagc
    15721 tgtcaaactc ggcttcgcta agatacccca gcgcaagggc cgcagctttt aaggtcagcc
    15781 cttctttatg cgcttttttg gcgatctccg cggctttgtc ataaccaatg tgggtgttaa
    15841 gcgcagtcac cagcatcagc gattcattga gtaattgatt gattcgctca cgattcggtt
    15901 caatacccac tgcgcagtgt ttgttaaaac tttccatgcc atctgccagc aagcgcaccg
    15961 attgcaggaa attatggatc accatcggac ggaagacgtt cagttcaaag ttaccggaag
    16021 cgccacccat gttgatcgcc acgtcgttcc ccattacctg acagcagagc atggttaatg
    16081 cttcacactg ggttgggttc actttccccg gcatgattga gctgcccggc tcattttccg
    16141 ggattgagat ttcaccaatt ccgcagcgcg ggccagaggc cagccagcgg acatcattgg
    16201 cgattttcat cagtgacgca gccaaccctt tcaacgcgcc gtgcgcctga accagggcat
    16261 cacaggtcgc aagcgcttca aatttgttcg gcgcggtaac aaacggtgca caggtaatga
    16321 ctgccagttc atctgctacg cgacgcgcat actccggatg ggtatttagt ccagtaccca
    16381 ccgctgtacc gccaagagcc agttccgcta cgtgaggcag gctgtattcg atatgtttga
    16441 gattatgctc gagcatcgct acccagccgg aaatctcctg ccctagtgtt agcggcgtgg
    16501 cgtcctgcaa gtgggttcga ccgattttga cgatatcggc aaatgcacgc gatttttcac
    16561 tcagtgtctg tgtcagggtt ttaagctgcg gaatgagttg cttgcgcagc gccagcagcg
    16621 ccgcaacgtg catcgccgtc ggaaagacat cgttggaact ttggcttttg ttcacgtcgt
    16681 cgttagggtg aactttacgt tccatcccgc gcacgccgcc gagtaattca ctggcccggt
    16741 tagccagcac ttcgttcatg ttcatgttac tttgcgtgcc ggagccggtc tgccagatag
    16801 ccagcgggaa ttcgtcgtca tgctgtcctg ccagtacttc atccgccgcc tgccgaatgg
    16861 cgctcgcttt ctcttcagac aacaagccta aatcttcatt aacttttgcc gctgcgcgct
    16921 tggttagcgc cagcgcatga atcagtgagg tgggcatttt ctccgtcgaa atgcggaaat
    16981 gctccagcga gcgttgagtt tgtgcgcccc acagcttatc tgccgggaca tcaatcgccc
    17041 ccatcgaatc tttttcgctg cgtactgtat tcatggcctg ctcctcacct gtttaatttt
    17101 ttctttctgt tttgctttcg ttaagcaact tttagctcac ttattattta ctatttgata
    17161 acaaatgttt ggtctttcgt gccatgtaaa aaaaccgccc cgaagggcgg ctctgtttat
    17221 ttcacacagc gagtgcactg tgtgagttgt atctgctgga agaagtcatt tcctttatca
    17281 tccacaagga taaacgccgg gaaatcttcc acttcaattt tccagatggc ttccattccc
    17341 agttccggat attcaacaca ttccaggctc ttaatacttc cctgcgccaa tacagcggcc
    17401 ggaccaccga tactgccaag gtagaagccg ccgtgttttt tacaggcatc cgtcacctgc
    17461 tggctgcggt tgcctttcgc cagcatgatc atacttccgc cctgcgcttg cagttgatcg
    17521 acataagaat ccatccgtcc ggcggtcgtt gggccaagag aaccggaggc ataaccttcc
    17581 ggcgttttgg ccggacccgc gtagtaaatc ggatgatctt taatgtactg cggcagccct
    17641 tcaccgttat ccatccgctc tttcagtttg gcgtgagcaa tatcacgacc gacgataatc
    17701 gtgccgttaa gcgataagcg tgtagaaacg ggatactgcg acaactgtgc gaggatctct
    17761 ttcatcggac ggttaaggtc aacgcgcacc gcttcgcctt ctcccgcttt acgcagctct
    17821 tcagggatat atttacctgg attatgttcc agtttttcga tccagatccc ctgacggttg
    17881 atcttcgctt tgatattacg gtcagcagag caggaaacac ccataccaac cggacaggat
    17941 gcgccgtgac gtggcaggcg aatcacgcgt atgtcgtgag cgaagtattt accaccaaac
    18001 tgcgcaccca gaccgagatt ttgcgcttcg atcagcaatt ctttttccag ttccacatcg
    18061 cggaatgcct gaccgtgctc attcccttcc gttggcagtt cgtcatagta tttagcggaa
    18121 gccagtttca ctgttttaag gttagtttct gcagaagttc caccaattac gaacgcaata
    18181 tgatacggag gacaggccgc cgtacccagc gtgcgcatct tctcaaccag gtaatttttc
    18241 agtttccccg gcgttagtaa cgctttggtt tcctgataga gatacgtctt gtttgccgaa
    18301 ccaccacctt tggcgataca gaggaatttg tactcgtcgc catcaacggc ataaagatcg
    18361 atctgcgctg gcagattggt gccggtattc acctctttat acatatccag cggcgcgttt
    18421 tgcgagtagc gcaaattatc ttcgatataa gtgttataga caccgcgcgc cagcgccgct
    18481 tcatcacagc caccggtcca tacacgctgc ccttttttac caacaataat cgcagtgccg
    18541 gtatcctgac aggttggcag aacacctttc gccgcgatgt cggagttgcg caggaattgc
    18601 agcgccacat atttatcatt ttcgctggct tccgggtcac gcagaatgtc ggccacctgt
    18661 tgttggtgag ccggacgcag cataaatgac gcatcatgaa atgcctgacg cgccaacaga
    18721 gttaacgctt cgggtgcgac tttcaaaatc tcctgccctt caaattcaga tacgctaacg
    18781 tgttcgctgg ttagcaggta atactcagta tcatcttttt tgagtggaaa aggagcctga
    18841 taatgaaagg gtttgtttga cattgttctc tcacttactg cctggtttgg ttatgctctg
    18901 ggcgggtgtt ccgttgccct gttaaaagcg agtaacaata tcctacacac ttttttaaca
    18961 aaaactgaga ctagtacgac tttttgcggc tccaggttac ttcccgtagg attcttgctt
    19021 taatagcggg attaatttcc acattaaaac agggattgat catgcaaaaa ctcattaact
    19081 cagtgcaaaa ctatgcctgg ggcagcaaaa cggcgttgac tgaactttat ggtatggaaa
    19141 atccgtccag ccagccgatg gccgagctgt ggatgggcgc acatccgaaa agcagttcac
    19201 gggtgcagaa tgccgccgga gatatcgttt cactgcgtga tgtgattgag agtaataaat
    19261 cgactctgct cggagaggcc gttgccaaac gctttggcga actgcctttc ctgttcaaag
    19321 tattatgcgc agcacagccg ctctccattc aggttcatcc aaacaaacgc aattctgaaa
    19381 tcggttttgc caaagaaaat gccgcaggta tcccgatgga tgccgccgag cgtaactata
    19441 aagatcctaa ccacaagccg gagctggttt ttgcgctgac gcctttcctt gcgatgaacg
    19501 cgtttcgtga gttttccgag attgtctccc tactccagcc agtcgcaggt gcacatccgg
    19561 caattgctca ctttttacaa cagcctaatg ccgaacgttt aagcgaactg ttcgccagcc
    19621 tgttgaatat gcagggtgaa gaaaaatccc gcgcgctggc gattttaaaa tcggcgctag
    19681 acagtcagca gggtgaaccg tggcaaacga ttcgtttaat ttctgaattt tacccggaag
    19741 acagcggcct gttctccccg ctgttactga atgtggtgaa attgaatcct ggcgaagcga
    19801 tgttcctgtt cgctgaaaca ccgcacgctt acctgcaagg cgtggcgctg gaagtgatgg
    19861 cgaactccga taacgtgctg cgtgcgggtc tgacgcctaa gtacattgat attccggaac
    19921 tggttgccaa tgtgaaattc gaagccaaac cggctaacca gttgttgacc cagccggtga
    19981 aacaaggtgc agaactggac ttcccgattc cagtggatga ttttgccttc tcgctgcatg
    20041 accttagtga taaagaaacc accattagcc agcagagtgc cgccattttg ttctgcgtcg
    20101 aaggcgatgc aacgttgtgg aaaggttctc agcagttaca gcttaaaccg ggtgaatcag
    20161 cgtttattgc cgccaacgaa tcaccggtga ctgtcaaagg ccacggccgt ttagcgcgtg
    20221 tttacaacaa gctgtaagag cttactgaaa aaattaacat ctcttgctaa gcttattaaa
    20281 ggcttataac accttcaggc ggccagtccg cctgatttca ttttatggat aatcattatg
    20341 aataaatcgc tggtagcggt aggcgtcatt gttgcgctag gcgtagtctg gacaggcggc
    20401 gcatggtata caggcaagaa gattgaaacc catctcgaag acatggtcgc gcaggcgaac
    20461 gcgcaactca aactgacagc tcctgaatcc aacctggaag tgagttatca aaactatcat
    20521 cgcggcgtat tcagcagcca gttgcaactg ttggtgaaac ccattgccgg gaaagaaaat
    20581 ccgtggatta aaagcggtca gagcgtcatc ttcaacgaat cggttgatca tggtcccttc
    20641 ccgcttgccc agcttaaaaa actgaacctg atcccgtcga tggcatcaat tcaaaccacg
    20701 ctggttaata acgaagtaag caaaccactg tttgatatgg caaaaggtga aacgcctttt
    20761 gagattaact cgcgcattgg ttacagcggt gattccagtt ccgatatttc gctcaagcca
    20821 ctgaattacg agcaaaagga tgaaaaagtc gcctttagcg gcggcgagtt ccagttaaat
    20881 gctgacagag acggcaaagc catctccctt tccggggagg cgcaaagtgg tcggatagac
    20941 gcagttaacg aatacaacca gaaagtgcag ttgaccttta ataatctgaa aaccgacggt
    21001 tccagcacgc tggcaagttt tggtgagcgc gtaggaaacc aaaaactgtc actggaaaaa
    21061 atgaccattt cagtggaagg caaagaactg gcactgctgg aaagcatgga gatcaacggt
    21121 aaatcggatc tggtcaatga cggtaaaacg atcaatagcc aactggatta ctcgctaaac
    21181 agcctgaagg tacagaatca ggatctgggc agcggcaagc tgactttaaa agtcggccag
    21241 attgatggtg aagcctggca tcagtttagc cagcaatata acgcgcaaac tcaggcgctg
    21301 ctggcacagc cagaaattgc caacaatccc gaactttatc aggagaaagt gacggaagcc
    21361 ttctttagcg ccctgccgct gatgttgaaa ggcgatccgg tgattactat cgcgccgcta
    21421 agctggaaaa acagtcaggg tgaaagtgcg ctgaatctgt cgctgttcct gaaagatccg
    21481 gcaacgacta aagaagcgcc gcaaacgctg gcgcaggaag tagatcgttc ggttaaatct
    21541 ctggatgcga aactgaccat tccggtggat atggcaactg agtttatgac tcaggtagcg
    21601 aagctggaag gttatcagga agatcaagcg aaaaaactgg cgaaacagca agttgaaggt
    21661 gcatcagcaa tggggcagat gttccgtctg accaccttgc aggacaatac catcaccacc
    21721 agcctgcaat atactaacgg tcagataacg ttaaacgggc agaaaatgcc actggaagat
    21781 ttcgttggta tgtttgcaat gctggcatta aatgttccgg tcgtacccgc tattccgcag
    21841 cagtaattca cagccaaaac gccggagatt tttctctccg gcgttatttt ttacttcagc
    21901 ataaagtcat actgaatact cattttcaca tcccagtaat tcaggcgatc gcccagcgta
    21961 ctgccatcgc gcttttcccg ccctttatcg gcagcaagaa agaaattcat cccttgtaac
    22021 ggcccttcgc tgacccgata gttcatacca cctaagatac ccgcgccttc aatccgcaca
    22081 tctcgtttac tggattcata gccgcgagtc acaatcggtg caaatatcag tgtaaattgc
    22141 ggcgttaaac gatagttgac gcccaattgc caggactgca ttccttcacg gttacgatca
    22201 agacttaatg aaaagggata gccaatgtcg gtatccacca cggcaccatt cattgcatgg
    22261 cgcaaataac caccactgcc aaatacgctt ccccacggcg cattccaggt caattgtcca
    22321 ctaaccaacg ccgtttcatt gggctggctg gtattacgac ttaaaccttc gagctgcgcg
    22381 taaaagccga gcaactcccc tttcaacgta taattcgggt cgagcggtat gcttttctcg
    22441 agaatacttt gtatctgatt ggaaacatct ttctgtaccg atgcccccag agaatagcga
    22501 ctggtatcat catgccaact ggcggctaaa aaagaacgtg cgcgatgttt atcgtatttt
    22561 cccgtctggc tgttatacac cccttcatca cgaataccgg ttgttgaatc agtccataaa
    22621 aatactcgtg aacgccacat tccccacaga tcaatatttg gtgttaaagc ccaataacct
    22681 aatgctcctt cagaaactgt cggtaatgta cgttgcgaac tgccagccat taacggaata
    22741 cgataaggta agctaccata actataatcc gtcccgaagc gacctaattt aagcaggcta
    22801 ttttcactgg gttttatttt tactgccccc agggcaaagc caaaactttt atcaccatca
    22861 agataccacc gggtactcat atcagcacga gcacctaatt tataaacata atacgccccg
    22921 ccttcaacgc caataatatt ggaataataa ccactattga aatcgagcaa tcctccctgt
    22981 acccatgcgt aaatatctct tcccgcgcca gcactgggct tatccgccct gcgcaattcg
    23041 tttttcagtc tgatgcgtag tcccgcttca tcgtcagcca gttgcgccgc ataagcagag
    23101 gtaaggccag aggcagccgt caggcaaata acggccatgg ccactatttt tctcatagtt
    23161 gcacctttga tgttctgatt atttttattg aatattaatt agtgatatcg ctgattaatt
    23221 gctgctgcac ttttttacga ttatcaattt caaccacgat ttctttgaat tttttatccg
    23281 tgagcggata aaaccagata ataacgaatg ccagtagcat aaatccgcaa ggtactaagg
    23341 caattgatgt gcggatgccc ataataactt ccggcgtttg cacctgattg gcgatatatc
    23401 cgcttaatcc caaaataaag gcaggaattg aacctccgat tgcctgaccg catttacggg
    23461 taaatgagaa tagtgaatag gtgagccctt caattcgcac gccggtcagg tattcaccgt
    23521 attctacggt atcagcttcc agtgcccaca tcacagtcat agtaacgccc tgaccaattg
    23581 aagcgatggc caacgcaacc aacgccaccg gcagtgacca gacggaaacc cagaagaata
    23641 gcagataacc gcaggttccc agcaaagcgc caatcaggaa ggtattcttt ttaccgatcc
    23701 tcgcgaccat ccccggcacc agcggtgccg atgccacagt accaaccagg ttttgcacca
    23761 gtaccagcac agtgaacagc ccggtatcat ttaacacata gcgcacgtag aacaacgacg
    23821 aggcgctgac cgcgaaggtc gaaatcagca cacacagcgc accgatgcac aacataaaca
    23881 gcgggcgatt ccgtttcagg gtttgcagac tgatcttcaa tgacggttgc gccacgatac
    23941 gtaccacatt ctcacgcgtc gatttgaagc agatgaagta aagcaccatt ccggcaatcg
    24001 ccagcacaat cgtccagaaa tggtataccg acaccatctc ttccgggctg gagttcttaa
    24061 tgctcggtcc tatcagaaat gccaggcaga caaaggttaa tgaagcggca atcccgcgag
    24121 ccgcgcccag acgggcgcgg gattgtggtt gttgggtcat cgcggtagca aatgaaccat
    24181 aaggaatatt caccaggctg tagcaaagcc cgaggcccat gtaggtcaaa tatgcataca
    24241 ctactttgct gctatggctc cagtcggtcg gtacccagaa taccagcacg ctgaagatca
    24301 ttaacggcgc agtaccgaag agtaaaaacg ggcggaattt tccccagcgg gtattcacac
    24361 tgtccaccac tcgtccggca aagacgtcgg cgaaggcatc gaatacccgc accagtaaca
    24421 gcatggtgcc cgccgcagcg gcaccgacgc cagcgacgtc ggtgtagtaa ctcaacagga
    24481 agagcgcccc cattgcgaag gcgaagttat tggcgacgtc accgaggctg tagccgacga
    24541 tggtgcgcca ggagagttgt tgattcattg tttgcctccc tgctgcggtt tttcaccgaa
    24601 gttcatgcca gtccagcgtt tttgcagcag aaaagccgcc gacttcggtt tgcggtcgcg
    24661 agtgaagatc cctttcttgt tacctccaac gcgcaatatg ccttgcgagg tcgcaaaatc
    24721 ggcgaaattc catacctgtt caccgacgac ggcgctgacg cgatcaaaga cgcggtgata
    24781 catatccagc catgcacact gatactcttc actccacatg tcggtgtaca ttgagtgcag
    24841 cccggctaac gtatccacgc cgtattcggt gatgataatc ggctgatgca gtttctcctg
    24901 ccaggccaga agttcttttt ccagtacctt ctctgccgtt tccaaatcgc cgctttggac
    24961 ataccatccg taataacggt tcaggcacag cacatcaaag agatcgctga tggtatcggt
    25021 gtgagcgtcg cagaacatta cattgacgca ggtgatcgga cgcgtcgggt cgagtttacg
    25081 cgttgcttcc gccagtggcg cgaaatattc ccgtgcacct tgcggacggg tatccggttc
    25141 gttggcaata ctccacatca ccacgcttgg gtggtttttg tcacgcgcta tcagctcttt
    25201 aatcgcctgt aagtgcgctt gctgagtttc cccgttgact gcctcttcgc tgtacagttc
    25261 tttcggcttg ttgcccgctt cgaaaccaat gcctaaagag aggttaaagc cgacagcagc
    25321 agtttcatca atcaccacga tgccatgttc atctgcccag tcgagcatct cttcagcgta
    25381 agggtaatgc gaggtacggt aggagttggc cccaatccag tccattaatg cgtggtcgtg
    25441 caccatcagc acgttatcga atcctttgcc acgcaagtcc gcatcttcat gacgaccaaa
    25501 gccagtaaag tagaacggtt tgtggttaat caggaactgt tcgcccttca ctgccactga
    25561 ccggatgccg acgcgaagcg ggtagatatc acactctgtc tggcttttgg ctgtgacgca
    25621 cagttcatag agataacctt cacccggttg ccagaggtgg ggattcacca cttgcaaagt
    25681 cccgctggta ccttgtccag ttgcaaccac ctgctgatcc gcatcacgca gttcaacgct
    25741 gacatcacca tttgctacca cctgccagtc aacagacgcg tggttacagt cttgcgcaac
    25801 atgcgtcacc acggtgatat cgtccaccca ggtgttcggc gtggtgtaga ccattacgct
    25861 gcgatggatc ccggcatagt taaagaaatc atggaagtaa gactgctttt tcttgccgtt
    25921 ttcatcggta atcaccattc ccggcgggat agtctgccag ttcagttcgt tgttcacaca
    25981 aacggtgata cgtacacttt tcccggcaat aacatacggc gtgacatcgg cttcaaatgg
    26041 cgtatagccg ccctgatgct ccatcacttc ctgattattg acccacactt tgccgtaatg
    26101 agtgaccgca tcgaaacgca gcacgatacg ctggcctgcc caacctttcg gtataaagac
    26161 ttcgcgctga taccagacgt tgcccacata attacgaata tctgcatcgg cgaactgatc
    26221 gttaaaactg cctggcacag caattgcccg gctttcttgt aacgcgcttt cccaccaacg
    26281 ctgatcaatt ccacagtttt cgcgatccag actgaatgcc cacaggccgt cgagtttttt
    26341 gatttcacgg gttggggttt ctacaggacg taacataagg gactcctcat tcaaataaaa
    26401 atactggtca acctttaatc tgattagtta accaattctg tgttgcacac agttatctgt
    26461 gagaaaccca tggaagagaa atggaaataa aaagaactga caccgcgatc aaaaaaccaa
    26521 aatctgcaat acccacagcg tgatattgat cgcattaatc tttaaaacag cgttgacaga
    26581 agccataaaa taagcaatcg aacgttcgtt cgagcatctt gagaacgata aactctcatg
    26641 acatatggct acggtgaata tttttggggg gaattctccg atcccggcta gattgtattt
    26701 tgtacaggga aattaaagta tgaaacgcgg gcacagttat caccagcccg cgaaatgaga
    26761 ggatcaggat gcggttaaga taccgccaat catcgcgcgt aatccctgag caaacgacgc
    26821 ttcacgcgcc aggtcttccg cttcgatatc ggccagtaca ccgtaggtca gatccagcag
    26881 taaacgtgaa gtcattgacg tgttgatgtc tgggcttatc tcgccttttt gctgggcatc
    26941 acgcatccgc tgggcaacaa attcagtgat cgtcatatgc ttatttttaa gcatggcggc
    27001 gacctgtggg ttacgcatcc cttccgccat aatttcgacc accagcgccc gttgcccaaa
    27061 ggcttcatgg gtgagagaga caatagactc gaccatatag tcaacaaaat gaatcccttc
    27121 aatcggttcc cggaaacggg ccagcgccct ctcctggtcc tgtaagataa tcgcctgaat
    27181 caaggcttct ttggagatga aatggtgata gagcgtcccg ggactaatgg cgcaggattt
    27241 acagatggct ttcatcgagg cactgtgaaa tccattttct gaaaaaatct ctctggcagc
    27301 attgaggatc cgggtccgtg tcggttgtgc ttcagtctgc atgttatcca tcattaaacc
    27361 attatagtta gcgtgaataa ctagtttacc gcgttgtcag gtgagcgcct atcattgctg
    27421 ttgaatagtg cgaaggcaca ctctattcgt atgcttttca tccagctatt ggttaaccaa
    27481 tagccgtgat ttggctgcca gttgtttagc agagacgttg atgaattgca cacgctatcc
    27541 cggtaaacaa cacatacggc tgtttaccgg ttcgttagtg tattaattga gctcctgtac
    27601 cccaccaccg gagacggtga gaatttgtcc gcttacccag ctggctgcag gcgagcaaag
    27661 gaacagcgcc gcgttagcaa tatcttgcgg ttggcccaga cgtctgatcg gcgtgtgctg
    27721 taacattttt tgttcaattt ctggtgtaat aacggatttc agggcatcgg ttaatattgc
    27781 ccccggcgca atgccattca cccgaatatt tttttcaccc aggtcaaatg ccatatttct
    27841 gaccagatga ctggccgcag ctttagatga tgcataggaa gtcatgttta tatttttatt
    27901 ttctgccgcc atagaagtga tggtcagaat aacgccaccg ccattttttt ccatttctgg
    27961 cgcaacaagt tgtgacagat ggaaaaaaga aaacacattc agttcataag cacggcgaaa
    28021 atctgccatt ggcatatcaa acggtttagg accaccgcca ccggcgttgt taaccaggat
    28081 atcaacttta cccagcttac tgacggcaaa gtctgccagt gcagagagtt cctgttcgga
    28141 agtaatatca caacggcagg caaatgcctg accacccagt tgttgaattt cgtctacaac
    28201 atggttagct gcgtcggcgt taatatcact gaccaccaca gatgcgccag ctgtcgcgaa
    28261 tgtaatggcg atttctttac caatacctgc acccgcacct gtgatgatgg cgcattttcc
    28321 gtcgagtctc aggttgtcag aattaaacac ataaacctcc tgtgaacttc agtaaaaata
    28381 aaaggcaccg cctgtgtgct acatttttta tcattaacct tgaggaaagg acactaccag
    28441 gtatagcaga taaagagagg attatttcgc cgcaatgagc cgggcgggaa taataagatt
    28501 gcgtgaatgc gtctcttcat gggtgatttt ttgcatcatg cgatccgcaa gtgtggtacc
    28561 aagttcccgc gcaggcgtgc tggcccaggt aacgggtata tcatcaagtg tggttggtgt
    28621 cgcatcggta aatgccgcca gcgaaacttg ttgctcaaag taacgatcga ctccgctttc
    28681 cccgctttgc cgccctgctt tgagcaaacc aaaccatgcc cccatcgcaa tagtttcgtt
    28741 atagcaaacc acagcactga tggtcgggtt atgacgtaaa agcgccgtga tagcttccgc
    28801 ggcttgcttc tggctggatg tgcactccag cacccaatcg ctgtgaaacg gcaggccaaa
    28861 ttttagtaga gttgcacaat agccccccac ccgttctgca cgggttaatg aggaactttg
    28921 ccctcccagc caggcgatcc gctgatgccc attgcgaatg agatgctccg tcaacaactg
    28981 tgcagcctgc atgttgtccg ggcgaaccgt atcaacatca tcaagataac tggcacggga
    29041 agcgaaaatc accgggatag ctttttcttc tgccatccgt cgcaggtcat cgctgcttcc
    29101 tgcagccccg gcaattacca caccatccac accctgattc agtaacagtg aaaaccgctg
    29161 tgccagctgc tcgccgtctt taccgccgtg aagcaaaaaa accatccgtc cctgcgcttc
    29221 cagagcttcc gtcaatccgg ccgtcaattc ggcgtaaaac ggcgcagata aatcacggac
    29281 gatcaaacca atgacgccgc tttgcccgcc gcgcagcgcc gacgcctggc gattgcgcac
    29341 aaatcccagc tcttcaatgg cggcgttaac gcgttctcct gtggcggtag agattcgccc
    29401 tttgccacta agcaccagcg aaacggtact taccgacacg cccgcagcca gcgcaacatc
    29461 atgaatggtt atttttttgg cggtagccat gcagatgacc tactccctga ttatgtgaca
    29521 gataaaacgt tttacctttt atttatctta tacccgctat tatcgttgcg taatgtgatt
    29581 tatgcctcac taaaatttga taaaacgttt tatcttctcg cgcaatttac tgaatccaga
    29641 ttgttctcta cgaggagtcg ttttatgacg gcgaaaacag caccgaaagt cacgctgtgg
    29701 gagttcttcc agcagttagg caaaaccttc atgttacccg tggcattatt gtcgttctgc
    29761 ggcattatgc tcggcattgg tagttctctt agcagccatg atgtcataac cctgatcccg
    29821 gtcctgggca accccgtgtt gcaggctatc tttacctgga tgagtaagat tggctcgttt
    29881 gcttttagtt tcctgcctgt catgttctgt atcgccatcc cgctgggcct ggcacgcgaa
    29941 aataaaggcg tagcggcatt cgctggcttc gtcggttatg cggtaatgaa cctcgcggta
    30001 aacttctggt tgaccaataa aggcattctg ccaaccacgg atgccgcggt tctgaaagcc
    30061 aataacatcc agagcattct tgggatccag tcgatcgata ccgggatcct cggtgcggtg
    30121 atcgccggta ttatcgtctg gatgctgcat gagcgtttcc ataatatccg cctgccggat
    30181 gcgctggcat tcttcggcgg tacgcgcttc gtaccaatta tctcctcgct ggtgatgggc
    30241 cttgtcggcc tggtgattcc attagtctgg ccgattttcg ccatgggtat tagcggcttg
    30301 ggccatatga taaacagcgc gggtgatttc ggaccgatgc tgtttggtac cggtgaacgt
    30361 ctgctgttgc cgtttggtct gcatcacatt ctggtagcat taattcgctt taccgacgca
    30421 ggcggcacgc aggaagtctg cggtcaaacc gtcagcggcg cactgaccat cttccaggcg
    30481 caattgagtt gcccgaccac tcacggtttt tctgaaagcg ccacgcgttt cctttcgcaa
    30541 ggtaaaatgc ctgcgtttct cggcggtctg ccgggtgcag cgttagctat gtatcactgc
    30601 gcgcgcccgg aaaatcgcca taaaattaaa ggtctgctga tttctggcct gatcgcctgc
    30661 gtcgttggcg gcactaccga accgctggaa ttcctgttcc tgttcgtagc gccagttctg
    30721 tatgtcatcc acgcgctgtt aaccggcctc ggcttcaccg tcatgtctgt gctcggcgtc
    30781 accatcggta ataccgacgg caatatcatc gacttcgtgg tgttcggtat tttgcatggt
    30841 ctgtcaacca agtggtacat ggtgccagtg gtggcggcaa tctggtttgt cgtttactac
    30901 gtcatcttcc gtttcgctat cacccgcttc aatctgaaaa ccccggggcg cgatagcgaa
    30961 gttgccagct caatcgaaaa agccgttgcc ggtgcgccgg gtaaatcagg ttacaacgtt
    31021 cctgcaatcc tcgaagcatt aggcggtgcc gacaatattg tcagcctcga taactgcatt
    31081 acccgtctgc gtttgtctgt gaaagatatg tcgcttgtta atgtgcaggc actgaaggac
    31141 aatcgggcaa ttggcgtagt acaacttaat caacataacc tgcaggttgt tatcgggcca
    31201 caagttcagt cagtaaaaga tgaaatggcc ggtctgatgc atactgtcca ggcataagga
    31261 taagatatgt tcgatttttc aaaggtcgtg gatcgtcatg gcacatggtg tacacagtgg
    31321 gattatgtcg ctgaccgttt cggcactgct gacctgttac cgttcacgat ttcagacatg
    31381 gattttgcca ctgccccctg cattatcgag gcgctgaatc agcgcctgat gcacggcgta
    31441 tttggctaca gccgctggaa aaacgatgag tttctcgcgg ctattgccca ctggttttcc
    31501 acccagcatt acaccgcaat tgatacgcag acggtggtgt atggcccttc tgtcatctat
    31561 atggtttcag aactgattcg tcagtggtct gaaacaggtg aaggcgtggt gatccacacg
    31621 cccgcctatg acgcatttta caaggccatt gaaggtaacc agcgcacagt aatgcccgtt
    31681 gctttagaga agcaggctga cgactggttt tgcgatatgg gcaagctgga agccgtgttg
    31741 gcgaaaccag aatgtaaaat tatgctcctg tgtagcccac agaatcctac cgggaaagtg
    31801 tggacgtgcg atgagctgga gatcatggct gacctgtgcg agcgtcatgg tgtgcgggtt
    31861 atttccgatg aaatccatat ggatatggtc tggggcgagc agccgcatat tccctggagt
    31921 aatatggcgc ggggagactg ggcgttgctg acgtcgggct cgaaaagttt caatattccc
    31981 gcgctgaccg gtgcttacgg gattatagaa aatagcagca gccgtgatgc ctatttatcg
    32041 gcactgaaag gacgtgatgg gctttcttcc ccttcggtac tggcgttaac tgcccatatc
    32101 gccgcctatc agcaaggtgc accgtggctg gatgccttac gcgtctatct gaaagataac
    32161 ctgacgtata tctcagataa aatgaacgcc gcgtttcctg aactcaactg gcagatcccg
    32221 caatccactt atctggcctg gcttgatcta cgtccgttga atattgacga caacgcgttg
    32281 caaaaagcgc ttatcgagca agaaaaagtc gcgatcatgc cggggtatac ctatggtgaa
    32341 gaaggtcgtg gttttgtccg tctcaatgcc ggctgcccac gttcgaaact ggaaaaaggt
    32401 gtggctggat taataaacgc catccgcgct gttcgctaac cccaattgcg caacgtaaaa
    32461 aatcgttgcg caatcgtgga tttttaccct gctttgtttt tataatggtg cgcactttta
    32521 tattcagaaa aagagtgcga ccatgattga taccaccctg ccattaactg atatccatcg
    32581 ccaccttgat ggcaacattc gtccccagac cattcttgaa cttggccgcc agtataatat
    32641 ctcgcttcct gcacaatccc tggaaacact gattccccac gttcaggtca ttgccaacga
    32701 acccgatctg gtgagctttc tgaccaaact tgactggggc gttaaagttc tcgcctctct
    32761 tgatgcctgt cgccgcgtgg catttgaaaa cattgaagat gcagcccgtc acggcctgca
    32821 ctatgtcgag ctgcgttttt caccaggcta catggcaatg gcacatcagc tgcctgtagc
    32881 gggtgttgtc gaagcggtga tcgatggcgt acgtgaaggt tgccgcacct ttggtgtgca
    32941 ggcgaagctt atcggcatta tgagccggac cttcggcgaa gccgcctgtc agcaagagct
    33001 ggaggccttt ttagcccacc gtgaccagat taccgcactt gatttagccg gtgatgaact
    33061 tggtttcccg ggaagtctgt tcctttctca cttcaaccgc gcgcgtgatg cgggctggca
    33121 tattaccgtc catgcaggcg aagctgccgg gccggaaagc atctggcagg cgattcgtga
    33181 actgggtgcg gagcgtattg gacatggcgt aaaagccatt gaagatcggg cgctgatgga
    33241 ttttctcgcc gagcaacaaa ttggtattga atcctgtctg acctccaata ttcagaccag
    33301 caccgtagca gagctggctg cacatccgct gaaaacgttc cttgagcatg gcattcgtgc
    33361 cagcattaac actgacgatc ccggcgtaca gggagtggat atcattcacg aatataccgt
    33421 tgccgcgcca gctgctgggt tatcccgcga gcaaatccgc caggcacaga ttaatggtct
    33481 ggaaatggct ttcctcagcg ctgaggaaaa acgcgcactg cgagaaaaag tcgccgcgaa
    33541 gtaacaaaat ggatggtgca aattgcacca tccatttttc atgcaaggca caaagtcgcg
    33601 cgatgtttgg cggattcgat gcccagctca atcaactcca ttacctggat tgcctggctt
    33661 gccggaaccg gattttcacc atcgccattt aacgcatcac gaatagccgc atagtaagcc
    33721 ggataattcc caggcaccgt caacagcgtt tcttcgacac gttcctcacc ttccacgcgg
    33781 gtaagtacgc catcacgcat atcgtagccc cagtcttcct gcggtagacg ctcgccattt
    33841 ttcagacgtt cttcctgtgg atcgaggcca tatttcacat aactgcctcg ggatccatgc
    33901 acgatatacc gtgctgactc agcggctgcc agcatggtac cgtgtaaaat gactcgccgc
    33961 tgtggatagg acaagatggc gtggaaataa tcggtcgact gcgctccggg ccgtaactgt
    34021 gccaaatcta ccgtcatgct gaccggtaaa ccaaaaagcg taatggcctg atcaagaaga
    34081 tgtggtgcta aatcgtacca gataccgctg cctgggccgc cctgttcacg ccaacgatcg
    34141 cgcacctgcg gacggaagcg gtcaaaatga gactcaaagt aagcaacttc acccagcacg
    34201 ccttccgcga gtaaaccttt tagcgtcaag aaatcgctat cccagcgacg gttatggaat
    34261 acagacagca cacgccccag gctttttgcc agcgcatcca gctctcgcgc ttgtgacagt
    34321 gtcacggtaa agggtttatc aacgaccaca tgtttacccg cctcaagcgc cgctttggct
    34381 aacgggaaat gggtatcgtt gggtgtagga atgacaatca ggtctatgtt gggatcgtta
    34441 aacagatgct tcggctcaga gacaaccgta accgttggcc agtcggcttt tacttttgtt
    34501 tcatcactgc tggagattac cgccagttcc agcccgggcg tgcccgcaat caggggcgca
    34561 tggaaggttt tgctcgcata accataccca atcaacccaa cacggatgtt gtcgctcatg
    34621 tcattgcctc tcattgaagt atgatggcta tttgacacta tcctttaccc acgctcaaca
    34681 gtttaataac ctgccagcaa taagggatgt tgtttaactt aagtcaaaaa aatagcgaat
    34741 tttccaacga caaaagctaa atatcgcaaa aacctcagta aaaatcttgc tggagctatt
    34801 attgctaagt aacatttacc ccctgaagtt aatggatcaa tcaagagaga tgtgggctgt
    34861 aatgaatcgt cttattgaat taacaggttg gatcgttctt gtcgtttcag tcattcttct
    34921 tggcgtggcg agtcacattg acaactatca gccacctgaa cagagtgctt cggtacaaca
    34981 caagtaagct ctgcacttgt ggagcgacat gctgcccgtc cgggtgcatg ttttcacttg
    35041 tcggatatta aaccaggaat ttattatctt gttcgatgtt gttggtgatt gtcagggata
    35101 gtaaagcgcg ttactctttt cagagcagtt tttcatccct gaatatttgc catcagcgca
    35161 ttcttattat tgtaataaca caagcgttca cttttggtga aattttatca tcactaataa
    35221 tacctacccc tttggtatta attattttat ggacctttta tgactgttca ggactactta
    35281 ttaaaatttc gcaaaatcag ttcactcgaa agtctggaaa aactctacga ccatcttaat
    35341 tacaccctga cggacgatca ggaactgatc aatatgtatc gtgctgccga tcaccgtcgc
    35401 gcagagctgg tttctggcgg gcgtttgttt gacctcggcc aggtaccgaa gtccgtctgg
    35461 cactatgtcc aataaagaaa gtagcgatcg gttataaaag ccttataata acgcccctaa
    35521 aattagattt gcctgtttgc gtcgctattc tggagaacct atgaccacca caacgccaca
    35581 acgtattgga ggctggttgc ttggcccgtt ggcctggctt ttagtcgctc tgttgagtac
    35641 gacgctggcg ttgttgctgt ataccgctgc gttatcttct ccgcaaacat ttcaaacgct
    35701 cggcggacaa gcactgacga cacaaatctt atggggcgtt tcttttatta ccgctatcgc
    35761 catgtggtat tacacactgt ggttgaccat cgctttcttt aaacgtcggc gttgtgttcc
    35821 taaacactat attatctggc tgcttatttc cgtattgctg gcagtaaaag cattcgcttt
    35881 ttcacccgtg gaagatggca tagccgttcg ccagttgctg tttactttgt tggcaaccgc
    35941 attgatcgtc ccctatttca aacgctcgtc gcgggtaaaa gcgacgtttg tgaatccgta
    36001 ataaccttac agttaacctg ttgtcgcctg ctctggatta acggataata ggcggctttt
    36061 ttatttcagg ccgaaaaatg actgactacc tgttactgtt tgtcggaact gtactggtca
    36121 ataactttgt actggtcaag tttctcggtc tctgtccgtt tatgggggtt tccaaaaaac
    36181 tggaaaccgc gatgggcatg gggctggcaa caacgtttgt gatgacgctg gcgtctattt
    36241 gcgcctggct tatcgatacg tggattttga tcccacttaa tctgatttac ctgcgcaccc
    36301 tggcatttat tctggtgatt gctgtggtcg tgcagttcac cgagatggtg gtgcgcaaaa
    36361 ccagcccggt gctttaccgt ctgctgggga tttttttgcc gcttatcacc accaactgtg
    36421 cagtgctcgg cgtggcgttg ctgaatatca atctcgggca caatttcttg cagtcggcgc
    36481 tgtacggttt ttccgccgct gtcggtttct cgctggtgat ggtgctcttc gccgccatcc
    36541 gcgaacgcct tgctgtggct gatgtcccgg caccttttcg cggtaatgcc attgcgttaa
    36601 ttaccgcagg tcttatgtct ctggccttta tgggctttag tggtttggtg aagttgtaat
    36661 gaatgctatc tggattgccg ttgccgccgt gagcctgctg ggcctggcgt ttggcgccat
    36721 tctgggttat gcctcccgcc gttttgcggt ggaagacgat ccggtcgttg agaaaattga
    36781 cgaaatctta ccgcagagcc agtgtggtca gtgcggttat cccggctgtc gcccctacgc
    36841 ggaagccatc agctgtaacg gtgaaaaaat caaccgttgc gccccaggtg gcgaagctgt
    36901 gatgctaaaa attgccgagt tgcttaatgt cgagccgcag ccgctggatg gcgaagcgca
    36961 agagctaacg cctgcgcgga tggtggcggt tattgatgaa aataactgta ttggctgcac
    37021 taaatgtatt caggcttgtc cggtagacgc catcgttggc gctacccgtg ccatgcatac
    37081 ggtaatgagt gatctctgta cgggctgcaa tttatgtgtt gatccgtgcc cgacgcactg
    37141 catctcgttg caaccggtcg cagaaacacc tgactcctgg aaatgggatc tgaacaccat
    37201 tcccgtgcgt atcattcccg tggaacacca tgcttaagtt attctctgca ttcagaaaaa
    37261 ataaaatctg ggatttcaac ggcggcatcc atccaccgga gatgaaaacc cagtccaacg
    37321 gtacacccct gcgccaggta ccactggcgc agcgttttgt tattccactg aaacagcata
    37381 ttggcgctga aggtgagttg tgcgttagcg tcggcgataa agtattgcgc ggccagccgc
    37441 ttacccgtgg tcgcggcaaa atgctgcctg ttcacgcgcc cacctcgggt accgttacgg
    37501 ctattgcgcc ccactctacg gctcatcctt cagctttagc tgaattaagc gtgattattg
    37561 atgccgatgg tgaagactgc tggatcccgc gcgacggctg ggccgattat cgcactcgca
    37621 gtcgcgaaga gttaatcgag cgcatacatc agtttggtgt agccgggctg ggcggtgcag
    37681 gattcccgac aggcgttaaa ttgcagggtg gcggagataa gattgaaacg ttgattatca
    37741 acgcggctga gtgcgagccg tacattaccg ccgatgaccg tttgatgcag gattgcgcgg
    37801 ctcaggtcgt agagggtatt cgcattcttg cgcatattct gcagccacgc gaaattctta
    37861 tcggcattga agataacaaa ccgcaggcga tttccatgct gcgcgcggtg ctggcggact
    37921 ctaacgatat ttctctgcgg gtgattccaa ccaaatatcc ttctggcggt gctaaacaat
    37981 taacctacat tctgaccggg aagcaggttc cacatggcgg gcgttcatcc gatatcggcg
    38041 tattaatgca aaacgtcggc actgcttatg cagtgaaacg tgccgttatt gatggcgagc
    38101 cgattaccga gcgtgttgta accctgactg gcgaagcaat cgctcgcccg ggcaacgtct
    38161 gggcacggct ggggacgcca gtgcgtcatt tattgaatga tgccggattc tgcccctctg
    38221 ccgatcaaat ggtgattatg ggtggcccgc taatgggctt taccttgcca tggctggatg
    38281 tcccggtcgt aaagattacc aactgtctgt tggctccctc tgccaatgaa cttggcgaac
    38341 cacaggaaga acaaagctgc atccggtgta gcgcctgtgc tgacgcctgc cctgcggatc
    38401 ttttgccgca acagttgtac tggttcagca aaggtcagca acacgataaa gctaccacgc
    38461 ataacattgc tgattgcatt gaatgtgggg cttgcgcgtg ggtttgcccg agcaatattc
    38521 ccctggtgca atatttccgt caggaaaaag ctgaaattgc ggctattcgt caggaagaaa
    38581 agcgcgccgc agaagccaaa gcgcgtttcg aagcgcgcca ggctcgtctg gagcgcgaaa
    38641 aagcggctcg ccttgaacga cataagagcg cagccgttca acctgcagcc aaagataaag
    38701 atgcgattgc tgccgctctg gcgcgggtga aagagaaaca ggcccaggct acacagccta
    38761 ttgtgattaa agcgggcgaa cgcccggata acagtgcaat tattgcagca cgggaagccc
    38821 gtaaagcgca agccagagcg aaacaggcag aactgcagca aactaacgac gcagcaaccg
    38881 ttgctgatcc acgtaaaact gccgttgaag cagctatcgc ccgcgccaaa gcgcgcaagc
    38941 tggaacagca acaggctaat gcggaaccag aagaacaggt cgatccccgc aaagccgccg
    39001 tcgaagccgc tattgcccgt gccaaagcgc gcaagctgga acagcaacag gctaatgcgg
    39061 aaccagaaga acaggtcgat ccgcgcaaag ccgccgtcga agccgctatt gcccgtgcca
    39121 aagcgcgcaa gctggaacag caacagacta acgcagaacc agaagaacag gttgatccgc
    39181 gcaaagcggc agttgccgcg gctattgccc gcgctcaggc caaaaaagcc gcccagcaga
    39241 aggttgtaaa cgaggactaa atggtattca gaatagctag ctccccttat acccataacc
    39301 agcgccagac atcgcgcatt atgctgttgg tgttgctcgc agccgtgcca ggaatcgcag
    39361 cgcaactgtg gttttttggt tggggtactc tcgttcagat cctgttggca tcggttagtg
    39421 ctctgttagc cgaagctctc gtactcaaac tacgcaagca gtcggtagcc gcaacgttga
    39481 aagataactc agcattgctg acaggcttat tgctggcggt aagtattccc cccctcgcgc
    39541 catggtggat ggtcgtgctg ggtacggtgt ttgcggtgat catcgctaaa cagttgtatg
    39601 gcggtctggg gcaaaacccg tttaatccag caatgattgg ttatgtggtc ttactgatct
    39661 ccttcccggt gcagatgacc agctggttac cgccacatga aattgcggtc aacatccctg
    39721 gttttatcga cgccatacag gttattttca gcgggcatac cgccagtggt ggtgatatga
    39781 acacactacg cttaggtatt gatggcatta gtcaggcgac accgctggat acatttaaaa
    39841 cctctgtccg tgccggtcat tcggttgaac agattatgca atatccgatc tacagcggta
    39901 ttctggcggg cgctggttgg caatgggtaa atctcgcctg gctggctggc ggcctgtggt
    39961 tgctatggca gaaagcgatt cgctggcata ttcccctcag cttcttagta acgctggcgt
    40021 tatgcgcaac gttgggctgg ttgttctcac cagaaacact ggcagcaccg caaattcatc
    40081 tgctgtctgg tgcgaccatg ctcggcgcat tctttatttt gactgacccg gttaccgctt
    40141 ctacgaccaa tcgtggtcgt cttattttcg gcgcgcttgc gggcttatta gtctggttga
    40201 tccgcagttt cggcggctat cctgacggcg tggcttttgc cgtcctgctg gcgaacatca
    40261 cggttcctct gatcgattac tacacgcgtc cgcgcgtcta cggccatcgc aaagggtaaa
    40321 ccatgctgaa aactatccga aaacacggca ttacgttagc gctatttgca gcgggttcaa
    40381 cagggttaac tgcggccatc aaccagatga ccaaaacgac gattgctgaa caggccagtc
    40441 tgcaacaaaa ggcattattt gatcaggtgc tgccagccga acgctataac aatgcgctgg
    40501 cacagagttg ctatctggta actgcgccag agttaggtaa aggtgagcat cgggtttaca
    40561 tcgccaaaca ggatgacaaa ccggtagccg ccgttctgga agcaaccgcg ccagatggct
    40621 attccggtgc gattcagctg ctggtgggag ccgattttaa cggcacggta cttggcacgc
    40681 gcgtgacaga gcaccacgaa acgccagggc ttggcgataa aatcgaactg cgcctttctg
    40741 actggatcac ccattttgcg ggtaaaaaaa tcagtggtgc agatgatgcg cactgggcgg
    40801 tgaagaaaga tggtggtgat ttcgaccagt tcaccggcgc gacgattact ccccgcgcgg
    40861 tggttaatgc ggtaaaacgc gccggattgt acgctcagac gttaccggca caactttctc
    40921 aacttcctgc ctgtggagaa taaagcgtga gcgaaattaa agacgttatt gttcaggggt
    40981 tgtggaaaaa caactctgcg ctggtccagt tgctcggcct ttgtcctctg ttggcggtca
    41041 cgtccactgc cactaacgct ctgggtttag gacttgcgac tacgctggta ctgacgctga
    41101 ccaacctgac catttcgacg ctgcgtcact ggacgccagc cgagatccgc attcccattt
    41161 acgtgatgat catcgcctcg gtggtcagcg ctgtacagat gctgatcaac gcctacgcct
    41221 ttggcctgta tcaatcatta gggattttta ttccgctgat tgtcactaac tgtatcgttg
    41281 tgggccgcgc tgaagccttc gccgccaaaa aaggtccggc gctttcggca ctggacggct
    41341 tttcaattgg tatgggcgca acctgcgcca tgttcgtgct gggttcacta cgcgaaatta
    41401 tcggcaatgg cacattgttt gacggtgcag atgcgctgtt aggtagctgg gcaaaagtat
    41461 tacgcgtgga aattttccac accgactccc ctttcctgct ggcgatgctg cctccaggtg
    41521 catttattgg cctgggactg atgctggcag gaaaatacct gattgatgaa agaatgaaaa
    41581 agcgccgtac tgaagcagct gccgaacgtg cattgccaaa cggtgaaaca gggaatgtct
    41641 gatgaacaaa gcaaaacgcc tggagatcct cactcgcctg cgtgagaaca atcctcatcc
    41701 caccaccgag cttaatttca gttcgccttt tgaattgctg attgccgtac tgctttccgc
    41761 tcaggcgacc gatgtcagtg ttaataaggc gacggcgaaa ctctacccgg tggcgaatac
    41821 gcctgcagcg atgcttgaac tgggcgttga aggggtgaaa acctatatca aaacgattgg
    41881 gctttataac agcaaagcag aaaatatcat caaaacctgc cgtatcttgc tggagcagca
    41941 taatggcgag gttccggaag atcgtgctgc gcttgaagcc ctgcccggcg taggtcgtaa
    42001 aacagccaac gtcgtattaa acactgcatt cggctggccg actattgctg tcgacacgca
    42061 cattttccgc gtttgtaatc gtactcaatt tgcgccgggg aaaaacgtcg aacaggtaga
    42121 agaaaagcta ctgaaagtgg ttccggcaga gtttaaagtc gactgccacc attggttgat
    42181 cctgcacggg cgttatacct gcattgcccg caagccccgc tgtggctctt gtattattga
    42241 agatctttgt gaatacaaag agaaagttga catctgaaga aaaggggtaa caccgattac
    42301 cccattgata acctttcttt atcctctttt aaaacttctt tttaa
//
LOCUS       DAZFXT010000029        41271 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.029, whole genome shotgun sequence.
ACCESSION   DAZFXT010000029 DAZFXT010000000
VERSION     DAZFXT010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41271)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41271)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41271
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.029"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..578
                     /locus_tag="ACHKW0_001667"
     CDS             54..578
                     /locus_tag="ACHKW0_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HFO7474518.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            662..1348
                     /gene="lpfB"
                     /locus_tag="ACHKW0_001668"
     CDS             662..1348
                     /gene="lpfB"
                     /locus_tag="ACHKW0_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial biogenesis chaperone LpfB"
                     /protein_id="HFO7474519.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALITTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNSNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     TGEMLTKPIN"
     gene            1372..3903
                     /gene="lpfC"
                     /locus_tag="ACHKW0_001669"
     CDS             1372..3903
                     /gene="lpfC"
                     /locus_tag="ACHKW0_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HFO7474520.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYNALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3914..4969
                     /gene="lpfD"
                     /locus_tag="ACHKW0_001670"
     CDS             3914..4969
                     /gene="lpfD"
                     /locus_tag="ACHKW0_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HFO7474521.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4975..5499
                     /gene="lpfE"
                     /locus_tag="ACHKW0_001671"
     CDS             4975..5499
                     /gene="lpfE"
                     /locus_tag="ACHKW0_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HFO7474522.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5752..7443
                     /gene="eptB"
                     /locus_tag="ACHKW0_001672"
     CDS             5752..7443
                     /gene="eptB"
                     /locus_tag="ACHKW0_001672"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HFO7474523.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7535..7611
                     /locus_tag="ACHKW0_001673"
     tRNA            7535..7611
                     /locus_tag="ACHKW0_001673"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7569..7571,aa:Pro,seq:cgg)
     gene            7646..7779
                     /locus_tag="ACHKW0_001674"
     ncRNA           7646..7779
                     /ncRNA_class="other"
                     /locus_tag="ACHKW0_001674"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8525..10132
                     /gene="dppA"
                     /locus_tag="ACHKW0_001675"
     CDS             8525..10132
                     /gene="dppA"
                     /locus_tag="ACHKW0_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HFO7474524.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10283..11302
                     /gene="dppB"
                     /locus_tag="ACHKW0_001676"
     CDS             10283..11302
                     /gene="dppB"
                     /locus_tag="ACHKW0_001676"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HFO7474525.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11312..12214
                     /gene="dppC"
                     /locus_tag="ACHKW0_001677"
     CDS             11312..12214
                     /gene="dppC"
                     /locus_tag="ACHKW0_001677"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HFO7474526.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12225..13208
                     /gene="dppD"
                     /locus_tag="ACHKW0_001678"
     CDS             12225..13208
                     /gene="dppD"
                     /locus_tag="ACHKW0_001678"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HFO7474527.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13205..14209
                     /gene="dppF"
                     /locus_tag="ACHKW0_001679"
     CDS             13205..14209
                     /gene="dppF"
                     /locus_tag="ACHKW0_001679"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding subunit
                     DppF"
                     /protein_id="HFO7474528.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGISFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIEMPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14239..15510)
                     /locus_tag="ACHKW0_001680"
     CDS             complement(14239..15510)
                     /locus_tag="ACHKW0_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transport family protein"
                     /protein_id="HFO7474529.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15986..16093
                     /locus_tag="ACHKW0_001681"
     CDS             15986..16093
                     /locus_tag="ACHKW0_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7474530.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16469..16576
                     /locus_tag="ACHKW0_001682"
     CDS             16469..16576
                     /locus_tag="ACHKW0_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7474531.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16952..17059
                     /locus_tag="ACHKW0_001683"
     CDS             16952..17059
                     /locus_tag="ACHKW0_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7474532.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            17435..17542
                     /locus_tag="ACHKW0_001684"
     CDS             17435..17542
                     /locus_tag="ACHKW0_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7474533.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17629..19308)
                     /gene="bcsG"
                     /locus_tag="ACHKW0_001685"
     CDS             complement(17629..19308)
                     /gene="bcsG"
                     /locus_tag="ACHKW0_001685"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HFO7474534.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(19305..19496)
                     /gene="bcsF"
                     /locus_tag="ACHKW0_001686"
     CDS             complement(19305..19496)
                     /gene="bcsF"
                     /locus_tag="ACHKW0_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0052324 - plant-type cell wall cellulose
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HFO7474535.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(19493..21064)
                     /gene="bcsE"
                     /locus_tag="ACHKW0_001687"
     CDS             complement(19493..21064)
                     /gene="bcsE"
                     /locus_tag="ACHKW0_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis c-di-GMP-binding protein
                     BcsE"
                     /protein_id="HFO7474536.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            21337..21525
                     /gene="bcsR"
                     /locus_tag="ACHKW0_001688"
     CDS             21337..21525
                     /gene="bcsR"
                     /locus_tag="ACHKW0_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsR"
                     /protein_id="HFO7474537.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            21537..22289
                     /gene="bcsQ"
                     /locus_tag="ACHKW0_001689"
     CDS             21537..22289
                     /gene="bcsQ"
                     /locus_tag="ACHKW0_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HFO7474538.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            22286..24904
                     /gene="bcsA"
                     /locus_tag="ACHKW0_001690"
     CDS             22286..24904
                     /gene="bcsA"
                     /locus_tag="ACHKW0_001690"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016760 - cellulose synthase (UDP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA];
                     GO_process: GO:0006011 - UDP-glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HFO7474539.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQIPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            24915..27254
                     /gene="bcsB"
                     /locus_tag="ACHKW0_001691"
     CDS             24915..27254
                     /gene="bcsB"
                     /locus_tag="ACHKW0_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HFO7474540.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMLGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETRSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            27261..28367
                     /gene="bcsZ"
                     /locus_tag="ACHKW0_001692"
     CDS             27261..28367
                     /gene="bcsZ"
                     /locus_tag="ACHKW0_001692"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex periplasmic
                     endoglucanase BcsZ"
                     /protein_id="HFO7474541.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            28349..31822
                     /gene="bcsC"
                     /locus_tag="ACHKW0_001693"
     CDS             28349..31822
                     /gene="bcsC"
                     /locus_tag="ACHKW0_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0030244 - cellulose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase complex outer membrane
                     protein BcsC"
                     /protein_id="HFO7474542.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSGDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            31904..33892
                     /gene="hmsP"
                     /locus_tag="ACHKW0_001694"
     CDS             31904..33892
                     /gene="hmsP"
                     /locus_tag="ACHKW0_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HFO7474543.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            34075..35361
                     /gene="dctA"
                     /locus_tag="ACHKW0_001695"
     CDS             34075..35361
                     /gene="dctA"
                     /locus_tag="ACHKW0_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HFO7474544.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            35582..37078
                     /locus_tag="ACHKW0_001696"
     CDS             35582..37078
                     /locus_tag="ACHKW0_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7474545.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(37174..38103)
                     /gene="kdgK"
                     /locus_tag="ACHKW0_001697"
     CDS             complement(37174..38103)
                     /gene="kdgK"
                     /locus_tag="ACHKW0_001697"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HFO7474546.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            38335..39102
                     /gene="pdeH"
                     /locus_tag="ACHKW0_001698"
     CDS             38335..39102
                     /gene="pdeH"
                     /locus_tag="ACHKW0_001698"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HFO7474547.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            39172..41232
                     /locus_tag="ACHKW0_001699"
     CDS             39172..41232
                     /locus_tag="ACHKW0_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7474548.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
BASE COUNT         9955 a        10303 c        11277 g         9736 t
ORIGIN      
        1 ccccgttgta tgaacaacat caagcatatt tatctatata aatggagtta taaatgaaaa
       61 aggttctgtt tgccttatct gcgcttgcgc tgacctctac ttccgtattc gcagctgatg
      121 cgggcgacgg tactgttaaa ttcaccggtg agattgttga cgccgcttgt gttgtgtctc
      181 cagacactca aaaacaagaa gttgttctgg gtcaggtgaa caagtctgta tttactgcta
      241 tgggtgataa atccacagca acgccgttca aaatcaaact ggaaaactgc gatatctcca
      301 ctttcaaaaa tgtggaaatc agctttaacg gcgttggcga tgcagacaac agcaaactgg
      361 tttctgtaag cactgaacca ggtgccgcaa ctggcgtagg tatcggtatt tatgataaca
      421 ccaataccct ggttgacctg aatactggta aatctgctac tgtcctgaaa gaaggtcaga
      481 ccgttctgta tttcaccgct aactatgtct ctaccaaaga tgcagtaacc acgggttacg
      541 gtaacgctga agtcgacttc aacctgactt acaactaatc gatcctgacg gaacgatcat
      601 ctctcaatgg caacagagac tgtgttgcca ttattcacac caatgactca gggaccgaat
      661 catgaaacgt attagctttg ctgtgctggc acttttaatg accgcacaac agtgttttgc
      721 tggtggcgta gccttaatca cgacgcgtgt tatttatgat ggcggcaaga aagaagcgtc
      781 gctgaccgtt gaaaaccata ataaaaatga agagtttttg atccagtcat ggattgatga
      841 tgtgaatagc aataaaaaaa caccgtttat tattacaccg ccattattca aacttgatcc
      901 tgataaaaat aacgtcttac gtatcgtcaa tattgaagat accttaccta aagatcgtga
      961 atccgtttac tggattaatg taaaggccat tcctgcgaaa agtgctgaaa atgaaaataa
     1021 aaacgtctta cagatagccg ttcgcacccg tttgaagttg ttctatcgtc caaaaggttt
     1081 gccgggaagc aacctcgagg ccatgaaaca gttacgtttc cagcgtcaaa ataaccaaat
     1141 tgtggttaat aacgactccg cttataacct gacctttaat cagttttcca ttaatggaca
     1201 aaagattgaa cgggcgggaa tggttctcgc gaaaggaaag ttaaacatta accttccagc
     1261 cggtacaggc aacgcgcacg aagtgaaata cagcattatt aatgatttcg gtgttactgg
     1321 cgaaatgctg acgaagccga ttaactgatc cttttacaag gcgcttttac tatgcattgg
     1381 atgcatttac ctctctatca ctacagagcg catttttcct tctctctgct ggcattaacg
     1441 attgccagct cgttacctgc gtatggcgga aaatttaacc ctaaatttct cgaaaacgtt
     1501 cagggtatcg atcaacacat cgacttgtcg gtatacgact cacctgtcgg ccagcaaatc
     1561 cccggtaaat accgggtttc ggtgtttgta aacgaagaaa aaatggcttc ccggacgctg
     1621 gattttagta ccgccagcga agcgaaacgc aaagccagcg gtgagtcgct tatgccttgc
     1681 cttagccggg tgcaactgga agaaatgggc gtgcgcgttg acagctttcc ggcgctgaag
     1741 atgtcaccac cagaagcctg cgtagcgttt gatgaaatca ttccccaggc caccagccgc
     1801 tttgatttta atactcagac gctgcatctt tcctttccac aggcggcaat gatgatgacc
     1861 gctcgcggca cggttgatcc ttctcgctgg gacgaaggaa tacctgcgct gttgctggat
     1921 tacagcttta gtggcagcaa tggacgcaat gaaggtacag gttcatcttc agattcgacc
     1981 agtgacagtt attacctgaa cctgcgcagc ggtttgaacg ttggtccgtg gcgtttacgt
     2041 aataacagta tctggaaccg taccgatggc aaaaaccaat gggataacgt tggtacatcg
     2101 ttaaaccggg caattattcc acttaaatca cagataactc tgggtgatac cgccacgcct
     2161 ggcgagattt ttgatagcgt acaaatgcgt ggggcgttac tggcatccga cgatgaaatg
     2221 ttgccggaca gccagcgtgg ctttgctccg gtagtgcgcg gcatcgccaa aagcaacgct
     2281 gaagtgagta tcgaacagaa cggctatgtc atttatcgca cttttgttca gccgggtgca
     2341 tttgagatta acgatctcta cgcaacctcc ggcagcggcg acctgacggt gatcattaaa
     2401 gaagccgacg gaagtgaaca acgttttatc cagccgtttt cggcggtggc gattttccag
     2461 cgtgaagggt atctgaaata cagtctggct gccggtgaat atcgtgctgg taattacgac
     2521 agcgataaac cacatttcgg gcagtttacc gccatgtacg gcttgccgtg gggtatgacc
     2581 gcctacggtg gtgcattgtt gtcggcagat tacaacgctt tagctctggg cctggggaaa
     2641 aacttcggta ccattggcgc agtatcggtg gatgtcactc aggcgaaaag tcagttacgc
     2701 aacaatgaaa aagaggaagg ccagtcttat cgcttcctct attcgaaaag ttttgagggt
     2761 ggcactgacc tgcgtctgtt gggctacaaa tactcaacca gcggctacta caccttccag
     2821 gaagcaactg atgttcgcag tgacgccgac agtgattacc gccgttatca caagcgcagc
     2881 cagatacagg gcaatattac ccagcaactg ggagactacg gctcggttta tttcaatatg
     2941 acgcagcagg attactggaa cgttgacggt aaggaaaact ccttaagcgc cggttatcac
     3001 ggtcacattg gtcgggtgaa ttacagcgtg gcctatacct ggacgagaag tccggaatgg
     3061 gaagaagacg atcgcttgtg gtcattcagt gtttccatcc ctctgggggg cgcgtggagc
     3121 agttatcgca tgactaccga ccagaatggc aagacgtcgc aacaggcctc tgttagcggc
     3181 acgttactgg aagatcgtaa cctgagctat aacgttcagc agggttacac cagtaatggc
     3241 gtaggctaca gtggcagcgt caacatgggg tatatgggtg gctctggtaa cattgacgta
     3301 ggctataact attctaaaga taaccaacag gttaactatg gcgtgcgcgg cggtgtcatc
     3361 gttcacagtg aaggtataac gctttcgcag cctttaggcg aaagtctggc gattgtttct
     3421 gcaccgggcg cgcgtggtgg acatgtggtt aacagcagcg gcgtagaagt tgactggatg
     3481 ggcaacgccg tggtgcctta tctcacacca tatcgtgaaa ccattgtgga attgcgtagc
     3541 gacacgttgg ggcaaaacgt tgaactacag gaagcgttcc agaaagtcgt gcctactcgt
     3601 ggtgcagttg tgcgttcccg ctttgatacc cgcgtcggtt accgtgtgct gatgagctta
     3661 aaacaggcga atggcaacgc agtaccgttt ggtgcaaccg cagcgttaat tgatgagagc
     3721 aaacctgcca gcagcattgt gggtgaagaa ggacagcttt atatcagcgg gatgccagaa
     3781 gagggcgaat tgcaggtgag ctgggggaac gagcaggcgc aacgttgccg tgtgccattc
     3841 cgcttgccag agaacaagga caatgcggct atcgtgatgg ttaatgcggt atgcgagaaa
     3901 taaggatatt tagatgaaaa ttaaaaattt gttggcaggt atggtgctgt tagggacgag
     3961 cacttttgct ggaaatatat gggctgcgga ttggggacca tgtcagacat caaatggggt
     4021 ggcccatgag tatagttttg atttcgtaca gactatccag gtaccttcag aaaataaggc
     4081 tgggaaaata ttaactcagc cctttgcttt aggtacaaaa tattccgctt attgtgaatg
     4141 cccggatccc attccagata atggagttgt gacttatttt aaaggtgtaa cgcttctgcc
     4201 tgagcctgga actgtagatg ggtactataa attcaataat tacattgata ttctcaccaa
     4261 aatatatgtc tacacacaat tagatatccc ggtgcctttt accgatcgaa gcaatggcac
     4321 ggcacagaaa gaatgtacgc cctatactgc taacaattgg gggacaggtg gtaaaggaag
     4381 tatcagtatt tatatttcac atccttttgt tgggcaaatg attattccta aaacacgagt
     4441 tgcgtccctc tttgggacaa aaaagaaagg tgtttacaat gatagtcagc ccatggccaa
     4501 tgtcagtatt tccgggagta ttacagttac tcaaggctgt gaacttgccg ctggcaccgt
     4561 actggatatt ccattcggag aatatcaggc ccatgatttt aaagggcgtg ccggacaacc
     4621 accacaaaat gttcagaagg tacaaaaaga attaagcttt gactgcaata atatttcaga
     4681 tggcgtcaaa atttatctca gtattgatgc aacaccaaat actgcctatc ccagcgcaat
     4741 tgatttaggc aacgccgatg ttggtgcagt cattgaggac ggtaaaggta atattcttaa
     4801 gccgaatgac agtaatagct tattagaaat gaacccaggc tcgctgtatg aagatgtcaa
     4861 acgcaaagcg acaaccacaa ttaccgctta tcccgttagc actaccggaa aattacccgc
     4921 tgccggggat tatagcggta ttgctaccat acatgtcgaa ctggagtaac agtcatgaag
     4981 cgaacaatac ttgcgttatg cctgctgcct ctttcctttt cgtcattcgc ggcaaccacg
     5041 gatttagggc cgaaaggaac attgaaattc acccttaaaa tcagccaggg tgcttgcgaa
     5101 ctggtgaaag acagcgttga ggttgacatg ggcaccgcgg ttctcaaaaa gccggtaaga
     5161 atcggaacgg aaattaatcc cactcctttt tccatcggcc tgaaaaactg tagcgaagtg
     5221 gtcagagcct acgtgactat ggacggtaca cccaatgcag acgactcaaa tctttttgcg
     5281 ctggatgcgg gtggcgctac aggcgttggg ctgaaaatca aaaccgctgc tggagtacaa
     5341 cagttcccgg aaaatacaac gccgacaccg gttgagtttg caattaacgg tgatggcgca
     5401 catcaactga actatatcgc cagctacgtg cctattcgcg ccgatgcaac tattggtcgc
     5461 gcagatgcaa cggtcgactt tactgtacag tacgaataat ttcacatctt aagggccagt
     5521 taactggccc tttttactgc tttttgtaac gctaaagtct cttttcaaac ttgcattttt
     5581 gtaaatttgt gcttcatgca cactctttcc ccacactttt tcgctttgct gtggtctact
     5641 tattcgcgcg tgtagatttt acttatctga ctacctccgc acttttcccc tgccgggcct
     5701 gaaaagccgc taagcagggt gttatcacct gtttgtccag ggtttgtttg catgagatac
     5761 atcaaatcga ttacacagca gaagctgagc tttttgcttg caatctatat tggccttttt
     5821 atgaatggcg cggtttttta tcgccgcttc ggcagctatg cgcacgattt taccgtctgg
     5881 aaaggcgttt ctgctgttgt tgaactggcc gccaccgtac tggtgacctt ctttttacta
     5941 cgtcttctat cgctgtttgg ccgccgcagc tggcgtattc tggcgtcgct ggtggtgctc
     6001 ttttccgcag gtgccagcta ttacatgacc ttccttaatg tggtcattgg ttatggcatc
     6061 atcgcttccg tcatgaccac cgatatcgac ctgtcaaaag aagttgttgg tctgaacttt
     6121 attctctggt taatcgccgt tagtgcattg cctcttatcc ttatctggaa taaccgctgt
     6181 cgctacacct tgctccgaca actgcgaacc ccagggcagc gtattcgcag cctggcggtc
     6241 gtcgtactgg cgggtattat ggtttgggca ccgattcgtt tgctggatat ccagcagaag
     6301 aaagtggaga gggcgaccgg cgttgatttg ccgagttatg gcggtgtcgt agcgaactct
     6361 tatctgccat caaactggct ttctgcgttg gggctgtatg cctgggcgcg ggtcgatgaa
     6421 tcttccgata ataattcatt gcttaatccg gcgaagaaat tcacttatca ggcaccgcaa
     6481 aacgttgatg acacttatgt cgtgtttatc atcggtgaaa ccacgcgttg ggaccatatg
     6541 ggcattttcg gctatgagcg taataccacg ccgaaactgg cccaggagaa aaatctggcg
     6601 gcgttccgtg gttactcctg tgataccgca accaaactct cactgcgttg catgtttgta
     6661 cgtcaggggg gcgcggaaga taatccgcag cgcacattaa aagaacagaa cattttcgcg
     6721 gttctgaagc agttaggatt cagttctgac ctctacgcta tgcagagcga aatgtggttc
     6781 tacagcaaca cgatggcgga caacattgct tatcgtgagc agattggtgc ggagccacgt
     6841 aatcgtggca agccggtaga tgatatgttg ctggtagacg aaatgcagca atcgctaggg
     6901 cgcaacccgg atggtaagca tctgatcatt ctgcatacca aaggttcgca ttttaactac
     6961 acccagcgtt atccgcgtag cttcgcgcag tggaagccgg aatgtattgg tgttgatagc
     7021 ggctgtacca aagcgcagat gatcaactcc tatgacaact cggtgaccta tgtggatcac
     7081 tttatctcca gcgtgattga tcaggttcgc gataagaaag cgattgtgtt ctacgcagct
     7141 gaccacggtg agtcaattaa tgaacgcgag cacctgcacg gcacgccgcg tgaactggca
     7201 ccgccggagc agttccgcgt accgatgatg gtctggatgt cagataaata tctggaaaat
     7261 ccggccaatg ctcaggcatt tgcgcagctg aaaaaagaag ccgacatgaa agtgccacgc
     7321 cgtcatgtag agctgtacga caccatcatg ggttgtcttg gctatacttc accggatggt
     7381 ggaattaacg aaaacaacaa ctggtgtcac atcccgcaga caaaagtggc agcggctaac
     7441 taagcggcct gctgactttc tcgccgatca aaaggcattt tgctattaag ggattgacga
     7501 gggcgtatct gcgcagtaag atgcgccccg cattcggtga ttggcgcagc ctggtagcgc
     7561 acttcgttcg ggacgaaggg gtcggaggtt cgaatcctct atcaccgacc aaattcgaaa
     7621 agcctgctca gcgagcaggc ttttttgcat ttgaagccca tgaggatgag aacctccggg
     7681 ggcggggagg ttcgactcga gcgaagcgag agaacgttgc gccagcaacg gcccgcaggg
     7741 cgagccacga agtggcgagt aatcctctat caccgaccaa attcgaaaag cctgctcaac
     7801 gagcaggctt ttttgcattt gtagctcatg aggatgagaa cctccggggg cggggaggtt
     7861 cgactcgacg tagatactga taagacgcgc cagcgtcgca tcaggcaacg gcaccgcact
     7921 tattgtcgga tgcggcgcga ccgccttatt cgacctacac accacacatt tccctgccaa
     7981 atttgcgccc catatctcac tatccattaa cggatttgtg acagaaacca ttgtcttttt
     8041 ttatatttgc ttaaatcttt ttttgcgtta cttatggctg accctggcat tttccgctgt
     8101 gcgttttagc gttcatggta ttaatcgctc agatatccac cttttcctca gatttttttc
     8161 attaaatgca taaaacttaa tcaccaaatg ttgctaaata ttaagctgat tgttctggtc
     8221 atgttggagt agcacaaatt tctctgctgc aaatagctgt ttgaagtttt tttaatcttt
     8281 gtttgtggat tctcaccgtt ggtgtaaatc ccggttggct gtattgacgt tttcacattc
     8341 tgttgacaga ttgtaggtca cgaggggcat tttatggagg atccgcactg ttacactgat
     8401 gttaattagt acggcatccc cacctcataa cgttgacccg accgggcaaa aaacaaaaaa
     8461 ggtcaggcag cgacaaccca ctgcaaaggg ttaaaacaac aaacatcaca attggagcag
     8521 aataatgcgt atttccttga aaaagtcagg gatgctgaag cttggtctca gcctggtggc
     8581 tatgaccgtc gcagcaagtg ttcaggctaa aactctggtt tattgctcag aaggatctcc
     8641 ggaagggttt aacccgcagc tgtttacctc cggcaccacc tatgacgcct cttccgtacc
     8701 gctttataac cgcctggttg aatttaaaat cggcaccact gaagtgatcc cgggcctcgc
     8761 tgaaaagtgg gaagtcagcg aagacggtaa aacctatacc ttccatctgc gtaaaggtgt
     8821 gaagtggcat gacaataaag aattcaaacc gacgcgcgaa ctgaacgccg atgacgtggt
     8881 gttctcgttc gatcgtcaga aaaacgcgca aaacccgtac cataaagttt ctggcggtag
     8941 ctacgaatac ttcgaaggca tgggcttgcc ggagctgatc agcgaagtga aaaaggtgga
     9001 cgacaacaca gttcagttcg tgctgactcg cccggaagcg ccgttcctcg ctgatctggc
     9061 aatggacttc gcctctattc tgtcaaaaga atatgctgat gcgatgatga aagccggtac
     9121 accggaaaaa ctggacctca acccaatcgg aaccggtcca ttccagttac agcagtacca
     9181 aaaagattcc cgtatccgct acaaagcgtt tgatggctac tggggcacca aaccgcagat
     9241 cgatacgctg gtcttctcta ttacccctga tgcttccgtg cgttacgcga aattgcagaa
     9301 gaatgaatgc caggtgatgc cgtacccgaa cccggcagat atcgctcgca tgaagcagga
     9361 taaatccatc aacctgatgg aaatgccggg gctgaacgtc ggctacctct cgtataacgt
     9421 gcagaaaaaa ccgctggatg acgtgaaagt tcgccaggca ctgacctacg cggtgaacaa
     9481 agacgcgatc atcaaagcgg tttatcaggg cgcgggtgta tcagcgaaaa acctgatccc
     9541 gccaaccatg tggggctata acgacgacgt tcaggattac acctacgatc ctgaaaaagc
     9601 gaaagccttg ctgaaagaag cgggtctgga aaaaggtttc tccatcgacc tgtgggcgat
     9661 gccggtacaa cgtccgtata acccgaacgc tcgccgcatg gcggagatga ttcaggcaga
     9721 ctgggcgaaa gtgggcgtgc aggccaaaat cgtcacctac gaatggggtg agtacctcaa
     9781 gcgtgcgaaa gatggcgaac atcagacggt aatgatgggc tggactggcg ataacgggga
     9841 cccggataac ttcttcgcca ccctgttcag ctgcgccgcc tctgaacaag gttctaacta
     9901 ctcaaaatgg tgctacaaac cgtttgaaga tctgattcaa ccggcgcgtg ctaccgacga
     9961 ccataacaaa cgcgttgaac tgtacaagca agcgcaggtg gtgatgcacg atcaggctcc
    10021 ggcactgatc atcgctcact ccaccgtgtt tgaaccggta cgcaaagaag ttaaaggcta
    10081 tgtggttgat ccattaggca aacatcactt cgaaaacgtc tctatcgaat aattaaaagc
    10141 catacccggt ggcgctgtgc ctctgcgtgc agcgccatcc ggcagcaata cttcattctc
    10201 tgtccgacaa gggcgggaat tgatttgtga gcaatacaga cacgcagttc caggctgcgg
    10261 gtcactatag agaatccggg ttatgttgca gtttattctc cgacgtttgg gactcgtcat
    10321 ccccacgttt atcggtatta cccttctcac atttgccttt gtccacatga tcccgggcga
    10381 tccggtgatg atcatggcag gcgaacgtgg gatctcccct gagcgtcacg cgcagctgct
    10441 ggctgaactc ggcttagata aaccgatgtg gcagcagtat ctccattaca tttggggcgt
    10501 aatgcacggc gatctaggca tttcaatgaa aagccgaatt ccagtatggg aagagttcgt
    10561 gccgcgcttt caggccacgc tggaacttgg cgtctgcgcg atgatttttg ctacggcagt
    10621 cggtattccg gttggtgtgc tggctgcggt taaacgcggt tccattttcg atcacacagc
    10681 ggttggcctg gcgctgaccg gctactcgat gccgatcttc tggtggggca tgatgctgat
    10741 catgctggtt tcggtgcact ggaacctgac gcccgtctcc ggtcgcgtga gcgatatggt
    10801 gttcctcgat gactccaatc cgttaaccgg ttttatgcta atcgacaccg ccatctgggg
    10861 tgaagacggc aactttatcg atgccgtcgc ccatatgatc ttgcctgcca ttgtgctggg
    10921 tactattccg ctggcggtca ttgtgcgtat gacacgctcc tcgatgctgg aagtgctggg
    10981 cgaggattac atccgcaccg cgcgcgccaa agggctaacc cgcatgcggg tgattatcgt
    11041 ccatgcgctg cgtaacgcga tgctgccggt ggtgaccgtt atcggcctgc aggtgggaac
    11101 attgctggcg ggggcgattc tgaccgaaac catcttctcg tggcccggtc tgggacgctg
    11161 gttgattgac gcactgcaac gccgcgacta tccggtagtg cagggcggcg tattgctggt
    11221 ggcgacgatg attatcctcg tcaacttgct ggtcgatctg ctgtacggcg tggtgaaccc
    11281 gcgtattcgt cataagaagt aaggggacat catgtcacag gttactgaaa ataaagtgat
    11341 tagcgcaccg gtgccgatga ccccgttaca ggagttctgg cactatttta aacgcaacaa
    11401 aggcgcggtc gtcgggctgg tttacgtcgt catcgtgctg ttcatcgcga tctttgccaa
    11461 ctggattgca ccctataacc cggcggaaca gttccgcgat gcactgctcg ccccgccagc
    11521 ctggcaggaa ggcggcagca tggcgcactt gctgggcacc gatgacgtag gccgtgatgt
    11581 gctgtcgcgc ctgatgtacg gtgcgcgcct gtcgctgctg gttggctgtc tggtagttgt
    11641 gttatcgctg attatgggcg ttattctcgg cctgatcgcc ggttactttg gcggcctggt
    11701 cgataacatc attatgcgcg tggtcgatat catgctggcg ctgccaagtc tgctgctggc
    11761 gctggtgctg gtggcaattt tcggcccgtc gattggtaac gccgcgctgg cactgacctt
    11821 cgttgccttg ccgcactatg tgcgcttaac ccgcgccgcc gtgctggtgg aagttaaccg
    11881 cgattacgtc accgcgtctc gcgtggcggg tgccggggcg atgcgtcaga tgtttattaa
    11941 catcttcccg aactgccttg cgccgctgat tgttcaggcg tcgctcggtt tctctaacgc
    12001 cattctcgat atggctgctc ttggtttcct cggcatgggg gcacagccgc caacgcctga
    12061 gtggggcacc atgctctccg acgtgttgca gttcgcgcaa agcgcctggt gggtcgtgac
    12121 cttcccgggt ctggcgatcc tgctgacggt gctggcattt aacctgatgg gtgacggtct
    12181 gcgtgacgcg ctcgatccca aactgaagca gtaagaggtt cgagatggcg ttattaaatg
    12241 tagataaatt atcggtgcat ttcggcgacg aaagcgcgcc gttccgcgcc gtagaccgca
    12301 tcagctacag cgtaaaacag ggcgaagtgg tcgggattgt gggtgagtcc ggctccggta
    12361 agtcggtcag ttcactggcg attatggggc tgattgatta tccgggccgc gtaatggcag
    12421 aaaaactgga gtttaacggc caggatttgc agcgtatctc agaaaaagag cgccgcaacc
    12481 tggtgggtgc cgaagtggcg atgatcttcc aggacccgat gaccagcctt aacccgtgct
    12541 acaccgtggg tttccagatt atggaagcga ttaaggtgca tcagggcggc aacaaaagta
    12601 cccgccgtca gcgagcgatt gacctgctga atcaggtcgg tattcccgat ccggcatcgc
    12661 gtctggatgt ttacccgcat cagctttccg gcggcatgag ccagcgcgtg atgatcgcca
    12721 tggcgattgc ctgtcggcca aaactgctga ttgccgatga accgaccacc gcgctggacg
    12781 tgaccattca ggcgcaaatc atcgaactac tgctggagct acagcagaaa gagaacatgg
    12841 cgctggtgtt aattacccat gacctggcgc tggtggcgga agcggcacat aaaatcatcg
    12901 tgatgtatgc aggccaggtg gtggaaaccg gtgatgcgca cgccatcttc catgcgccgc
    12961 gtcacccgta tactcaggca ttgctgcgtg cgctgccaga atttgctcag gacaaagaac
    13021 gtctggcgtc gttgccaggt gtcgttcccg gtaagtacga ccgcccgaac ggctgcctgc
    13081 ttaacccgcg ctgcccctat gccactgaca gatgtcgcgc tgaagaaccg gcgctgaata
    13141 tgctcgctga tgggcgtcag tccaaatgcc attacccact tgatgatgcc gggaggccga
    13201 cactatgagt acgcaagagg ccacctcgca acaaccgctg ttgcaggcta tcgacctgaa
    13261 aaaacattat ccggtgaaga aaggcatgtt cgcgccggaa cgtctggtta aagcgctgga
    13321 tggcatttcg tttaaccttg aacgtggcaa aacgctggca gtagtgggcg aatctggctg
    13381 cggtaaatcg accctcggtc ggttgctgac gatgattgaa atgcccaccg gtggcgagct
    13441 gtattaccag gggcaggatc tgcttaagca cgatccgcag gcgcagaagc tgcgtcggca
    13501 gaaaatccag atcgtcttcc agaaccctta cggttcgctg aatccgcgta aaaaagttgg
    13561 gcaaattctc gaagagccgc tgcttattaa caccagctta agcaaagaac agcgtcggga
    13621 aaaagccctg tcgatgatgg cgaaagtcgg cctgaaaacc gagcactatg accgctatcc
    13681 gcatatgttc tccggcggac aacgtcagcg tatcgctatt gcccgtggtc tgatgctcga
    13741 cccggatgtg gtgattgccg atgagccggt ttctgcgctg gacgtctcag tgcgtgcgca
    13801 agtgctgaat ctgatgatgg atttacagca ggaactgggg ctttcttatg tctttatctc
    13861 ccacgacctg tcggtggtgg agcacattgc cgatgaagtg atggtgatgt acctgggccg
    13921 ctgcgtggag aagggaacga aagaccaaat cttcaataac ccgcgccatc cgtacactca
    13981 ggcgctactc tccgcgacgc cgcgcctgaa cccggacgat cgccgcgaac gcatcaaact
    14041 caccggtgaa ctgccaagcc cgctgaatcc accgccgggt tgcgccttca acgcccgctg
    14101 tcgtcggcgc ttcggcccct gcacccagtt gcagccgcag ctaaaagact acggcggcca
    14161 actggtagct tgttttgctg ttgatcagga tgaaaatccg cagcgttaac tcacaaaacc
    14221 ggggccatgc gctccggttt actcaatcag cttcaacagt ggcgacaagc atagcaaaat
    14281 gccgtaaagc agaattagcc aggctttaaa tccgcgaagt ttttccagtt gtgcgacttt
    14341 atagatcagg aaaaacggaa tgaggcacga cacaatgcca tataacgggc ttcccaactg
    14401 aaagaacacc agcaccgata cacgaaacga aacccaaatc gtcaatgtaa tgacgataaa
    14461 agcgcagatc gccagagtca gcatgcgtga gttaattttc ttggtatcaa taattcggct
    14521 taacagattg agaataatgc ctttaatagc ctcgtggaaa ccgagataaa tgccaaagaa
    14581 tgcggtcagt acggcaaaga tattcagcac cgtagaggtg atatgaatga tatgtccagg
    14641 gatcacctgc gcggccagcg ccagtgccga gatattttgt tcaaaggcag aaacggcttc
    14701 ttcgtggcta attgagaagg taaacgaaaa ggcaaaaaac aggatcaccg cgatgagcgt
    14761 gatataacta attcggtggg tacgcagcgc gagccgggtt gccagtactt tatccgcttc
    14821 ccgtttacga taggcaatat tcattgggtt aagtacctga ataaatactg cagaaaagaa
    14881 gcaaaatgga atggtaagca agacatcgcg gaaaaagacg gaggcttgcg ggaaggcggt
    14941 tatattggcg aaattccagt gcgggatcat cgcaaaaccg aacacgacaa taatccctac
    15001 tttgaccacc accattggcc cggaaatctt aaacagtaat cgttcaccac cagacgcaat
    15061 cgccaccagt acggcgaaaa tagcgacttt ataaagtaga gattgtgaaa gatcggcatc
    15121 ggttaaaccg aaggttttca ggtacgaggc gctgtcgaaa accacggaga gagagtagat
    15181 aaaaatcccg tggataatca tcaaaaagta gataaccccg aggaaaattc cccagttctt
    15241 ccccaggtaa tgactgataa tatcggtgta gtcattacag gaatcgcttt cagaaagggt
    15301 ttttaaataa atgtcctgca ctacccaggt ggcaggataa gcaatgatcg ctgcggtaat
    15361 aaatacccaa attcccttca agccaatttg tactggcatc agcacggttc cggcaccaat
    15421 cgccatgcct atgcataata aaacccagcc gaagtcgtag cgtgtaaacg gcaatttctg
    15481 attgtgtttc gatagtgtgt tgtgctgcat aaagtcctgt ccagaaagaa gtcggcctcg
    15541 caaaaatgaa taaacccagt ttctcttgcg caacaggtgg agaagtcgaa tacgcagtat
    15601 gtaaaaaatg aggcaacaga ttcactgggg aggtcgcgag gaggcgcaat tctacaaaat
    15661 tgcgcatttc actaccagtc tggagtcagc ccggatatga actgcaacta ttgcaaataa
    15721 agaaattaag ctggaaggca tcgcgcaatg aggaagattg ccgggtgctg acagtaaaaa
    15781 gcaatcgtaa aatcagcggc aagccgaaag ggttccggtg aggcgcaatg ttgcgggggc
    15841 tttatccctg gtggcattgg ttgctggaga gagaaaaccc ccgcacgttg caggtatgta
    15901 cctgacaaca taacgggggc taatcttgaa cctagaccac tcaagaatag ccgcgaaacg
    15961 ttgtcattac aacacaggcg gctatatgac gctcgcagag ctgggcatgg ccttctggca
    16021 tgatttagcg gctccggtca ttgctggcat tcttgccagt atgatcgtga actggctgaa
    16081 caagcggaag tagcgtgtca tgtgggcgtc aggctgccgt aatggcaatt tgcgcccgga
    16141 ccaggccgca ggagtgaaac tctgcggcct ttttcgtaag aaaaatgcaa cggttgcaag
    16201 cggataatat tctgcggaat gcgcagcaca gagtgaagat gagacggatt ccggaacaga
    16261 aaaacagtcg tagaatcagc gtcaagccgg aaggattccg gtgaggcgca atgttgcggg
    16321 ggcttaatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg ttgcaggtat
    16381 gcacctgaca acaccacggg ggccattctt gcatccaaac aatacaagaa tagccgcgaa
    16441 acgttgtcat tacaatacag gcggctatat gacgctcgca gaactgggca tggccttctg
    16501 gcatgattta gcggctccgg tcattgctgg tattcttgcc agtatgatcg tgaactggct
    16561 gaacaagcgg aagtaacgtg ccatgcgggc gtcaggctgc cgtaatggca atttgcgccc
    16621 ggatcaggcc gcaggggtga aactctgcgg cctttttcgt aagaaaaatg caacggttgc
    16681 aagcggataa tattctgcgg aatgcgcagc acagagtgaa gacgtgacgg attccggaac
    16741 agaaaaacag tcgtagaatc agcggcaagc cggaagggtt ccggtgaggc gcaatgttgc
    16801 gggggcttta tccctggtgg cattggttgc tggagagaga aaacccccgc acgttgcagg
    16861 tatgcacctg acaacatcac gggggccatt cttgaacctg aacaactcaa gaatagccgc
    16921 gaaacgttgt cattacaaca caggcggcta tatgacgctc gcagaactgg gcatggcctt
    16981 ctggcatgat ttagcggctc cggtcattgc tggcattctt gccagtatga tcgtgaactg
    17041 gctgaacaag cggaagtaac gtgtcatgcg ggcgtcaggc tgccgtaatg gcaatttgcg
    17101 cccggaccag gccgcagggg tgaaactctg cggccttttt cgtaagaaaa atgcaacggt
    17161 tgcaggcgga taatattctg cggaatgcgc agcacagagt gagtaagtga tggatttcgg
    17221 aacagaaaaa cagtcgtaga atcagcggca agccggaaag gttccggtga ggcgcaatgt
    17281 tgcgggggct ttatccctgg tggcattggt tgctggagag agaaaacccc cgcacgttgc
    17341 aggtatgcac ctgacaacat aacgggggct aatcttgaac ccaaacaact caagaatagc
    17401 cgcgaaacgt tgtcattaca acacaggcgg ctatatgacg ctcgcagagc tgggcatggc
    17461 cttctggcat gatttagcgg ctccggtcat tgctggcatt cttgccagta tgatcgtgaa
    17521 ctggctgaat aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt
    17581 gcgcccggat caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta
    17641 aggcacccag tcgccgccgt tcaggcgaac gtacggttta tcctggtatt gaataactac
    17701 tgcatttgag ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca
    17761 gtcaacattg tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat
    17821 agccaggaag ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat
    17881 gccgaagaat ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag
    17941 gccagatacc tgcattctgt cgcccttaag cgcgccgccg tgttccggca ccacgaccac
    18001 catcacttta cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa
    18061 tttctgcgcc cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc
    18121 atgcagtgga agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac
    18181 gtccagccag cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag
    18241 caaaataacc ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg
    18301 aacttctttc aaaaaaccgc cgaactggcc gttatgcccc atcatcaggt gctgggtaaa
    18361 gcccagtttc gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt
    18421 agtgtgcgaa gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta
    18481 ggaggtggcg gagttaaagt tcttgaactc aatatcgaaa tgcgaccaca gtggatgcga
    18541 catcaacccg gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag
    18601 ttcaaatggc tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc
    18661 cgcgttatag aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg
    18721 tgcgggcata tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt
    18781 accacccgtc gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg
    18841 tcccgccagg gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg
    18901 aatccattgt gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg
    18961 ccagttaata aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg
    19021 cgaaccctgg ctcattatgc tttccgggcc aggcaaccag gtgtcatgcc agaacaaagc
    19081 aaagccgatc ggcagggcaa tccagtggcg caagcgatgc aggctgtagc gcggaatggg
    19141 catcagcaga aacgcggcaa acaccaaatt gaggagcgga tggaagttaa gatatcccgc
    19201 ccacaacagg ccgaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta
    19261 ttgccagagg gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc
    19321 ctggcttttt ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag
    19381 aacaaacgta aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg
    19441 aaaaatatca gtgcgcaaac gacaataatt tcaatgatat cgctgatggt catcatgatg
    19501 agcgctccac agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac
    19561 catcgtgctc ggcattgatg accggtttag aactttgcgt taaaggcagc ggcatgcccc
    19621 attgttctgg ggcaagcaag cgcatctgca ccagctcggc actgatttga tcatcttcaa
    19681 accagaccat acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg
    19741 cggtatccag atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa
    19801 tggtcatgat atcgccggta cggttagggc gacacagcgt cagggcttgt tcaacgcgga
    19861 tacccggtac cggacgtagg gcaaccagaa cgcctttacc gtgggcaggt aatagagggt
    19921 tattcatcat gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt
    19981 tgagcggctg ggtcattgac agcaaggtag tgatatcttc cggcacatag cgactaaact
    20041 tctgcccttg cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa
    20101 taaccatatt tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg
    20161 tattttcccg cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc
    20221 tgcgggccag tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct
    20281 gagcggtacg ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg
    20341 atagcggcgg cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc
    20401 gtggttggat ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa
    20461 cgctaagctg ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca
    20521 aatgttggtc accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa
    20581 gcaacaatga aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat
    20641 ggtttaacct gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa
    20701 tgttttgcca tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag
    20761 aacactgcaa atcacgggtc aaatagtata gacctttttc atgatttagc attgaaaata
    20821 attttatttt ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt
    20881 ccatgctaat gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca
    20941 gactgatagc atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat
    21001 gtcgcagctc atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc
    21061 tcatgtcacc attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc
    21121 attgaaatta aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg
    21181 tgattttagt cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag
    21241 caaattcccg tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc
    21301 atgaacggta cggttatttc atagggatca agcaaaatga ataacaatga accagatact
    21361 ctgcctgatc ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca
    21421 ttttcactgc ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca
    21481 ttaaaacgct ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg
    21541 ccgtactggg attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat
    21601 tagcctggtc attacaaatg ttgggagaaa atgttctggt ggtcgatgcc tgcccggaca
    21661 acttgttgcg cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga
    21721 tgctggatgg ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc
    21781 tgccttttgg tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc
    21841 tgagcgatat ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt
    21901 taatcgactt accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgatc
    21961 actcgctggc aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc
    22021 tgccggatgg cgcacatatt ttgattaata acttccgtat tggcagtcag gttcaggacg
    22081 atatttacca gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg
    22141 atgaagcgat ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg
    22201 cgctggcggc tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc
    22261 tgaaaacgcc agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc
    22321 ggtcaacgcg cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt
    22381 cagcgcgacg ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca
    22441 cccgcgctgg cagcgtattc gcgcagaaca taaaaacctg tatccgcata tcaacgcctc
    22501 gcgtccgcgt ccgctggacc cggtccgtta tctcattcaa acatgctggt tactgatcgg
    22561 tacatcgcgc aaagaaacgc cgaaaccgcg caggcgggca ttttcaggtc tgcagaatat
    22621 tcgtggacgt taccatcaat ggatgaacga gctgcctgag cgcgttagcc ataaaacaca
    22681 gcatcttgat gagaaaaaag agctcggtca tttgagtgcc ggggcgcggc ggttgatcct
    22741 cggtatcatc gtcaccttct cgctgattct ggcgttaatc tgcgttactc aaccgtttaa
    22801 cccgctggcg cagtttatct tcctgatgct gctgtggggt gtagcgctga tcgtacggcg
    22861 gatgccgggg cgcttctcgg cgctaatgtt gattgtgctg tcgctgaccg tttcttgccg
    22921 ttatatctgg tggcgttaca cctctacgct gaactgggac gatccggtca gcctggtgtg
    22981 cgggcttatt ctgctcttcg ctgaaacgta cgcgtggatt gtgctggtgc tcggctactt
    23041 ccaggtagta tggccgctga atcgtcagcc ggtgccattg ccgaaggata tgtcgctgtg
    23101 gccgtcggtg gatatctttg tcccgactta caacgaagat ctcaatgtgg tgaaaaatac
    23161 catttacgcc tcgctgggta tcgactggcc gaaagacaaa ctgaacatct ggatcctcga
    23221 tgacggcggc agggaagagt ttcgccagtt tgcgcaaaac gtgggggtga aatatatcgc
    23281 ccgcaccact catgaacatg cgaaagcggg caacatcaac aatgcgctga aatatgccaa
    23341 aggcgagttc gtgtcgattt tcgactgcga ccacgtacca acgcgatcgt tcctgcaaat
    23401 gaccatgggc tggttcctga aagagaaaca gctggcgatg atgcagattc cacaccactt
    23461 cttctcgccg gacccgtttg aacgcaacct ggggcgtttc cgtaaaacac cgaacgaagg
    23521 cacgctgttc tatggtctgg tgcaggatgg caacgatatg tgggacgcca ctttcttctg
    23581 cggttcctgt gcagtaattc gtcgcaaacc attggatgaa attggcggta ttgctgtcga
    23641 aacggtaacc gaagatgcgc acacgtctct gcgcctgcac cgtcgtggct acacctctgc
    23701 atatatgcgt attccgcagg cggcggggct ggcgaccgaa agtctgtcgg cgcatatcgg
    23761 tcagcgtatt cgctgggcac gcgggatggt gcaaatcttc cgtctcgata acccgcttac
    23821 cggtaaaggg ctgaaattcg cccagcggct gtgctacgtc aacgccatgt tccacttctt
    23881 gtcgggcatt ccacgactga tcttcctgac tgcgccgctg gcgttcctgc tgcttcatgc
    23941 ctacatcatc tatgcgccag cgttgatgat cgccctgttc gtgctgccgc atatgatcca
    24001 tgccagcctg accaactcga agatccaggg caaatatcgc cactctttct ggagtgaaat
    24061 ctacgaaacg gtgctggcgt ggtatatcgc accaccgacg ctggtggcgc tgattaaccc
    24121 gcacaaaggc aaatttaacg tcaccgccaa aggtggactg gtggaagaag agtacgtcga
    24181 ctgggtgatc tcgcggccct acatcttcct tgttctactc aacctggtgg gcgttgcggt
    24241 aggcatctgg cgctacttct atggcccgcc aaccgagatg ctcaccgtgg tcgtcagtat
    24301 ggtgtgggtg ttctacaacc tgattgttct tggcggcgca gttgcggtat cggtagaaag
    24361 caaacaggta cgccgatcgc accgcgtgga gatgacgatg cccgcggcaa ttgcccgcga
    24421 agatggtcac ctcttctcgt gtaccgttca ggatttctcc gacggtggtt tggggatcaa
    24481 gatcaacggt caggcgcaga ttctggaagg gcagaaagtg aatctgttgc ttaaacgcgg
    24541 tcagcaggaa tacgtcttcc cgacccaggt ggcgcgcgtg atgggtaatg aagttgggct
    24601 gaaattaatg ccgctcacca cccagcaaca tatcgatttt gtgcagtgta cgtttgcccg
    24661 tgcggataca tgggcgctct ggcaggacag ctacccggaa gataagccgc tggaaagtct
    24721 gctggatatt ctgaagctcg gcttccgtgg ctaccgccat ctggcggagt ttgcgccttc
    24781 ttcggtgaag ggcatattcc gtgtgctgac ttctctggtt tcctgggttg tatcgtttat
    24841 tccgcgccgc ccggagcgga gcgaaacggc acaaccatcg gatcaggctt tggctcaaca
    24901 atgatgataa cgcgatgaaa agaaaactat tctggatttg tgcagtggct atggggatga
    24961 gtgcgttccc ctctttcatg acgcaggcga cgccagcaac gcaaccactg atcaatgctg
    25021 agccagctgt agccgcccag acggaacaaa atccgcaggt ggggcaagtg atgctgggcg
    25081 tgcagggcgc tgatgcgcca gtcgtggcgc agaacggtcc ttcgcgtgat gtgaagctga
    25141 cctttgcgca aattgcgccg ccgccgggca gcatggtgct acgtggcatt aacccgaacg
    25201 gcagcattga gtttggtatg cgcagcgatg aagtggtgac gaaggcgatg ctcaacctcg
    25261 aatacacgcc atcgccatcg ttactgcctg tccagtcgca gttaaaggtt tatctcaatg
    25321 atgagctgat gggcgtgctg ccagtgacca aagaacagtt gggtaaaaaa acgctggcgc
    25381 aaatgcccat taacccactg tttattaccg acttcaaccg tgtgcggctg gagtttgtcg
    25441 gccattatca ggacgtgtgc gaaaacccgg ccagcaccac gctttggctg gatgttgggc
    25501 ggagcagtgg actggatctg acctatcaga ccctgaatgt gaagaatgac ctgtcacact
    25561 tcccggtgcc attctttgat ccgcgtgata accgcaccaa caccttgccg atggtctttg
    25621 cgggtgcgcc ggatgttggg ctgcaacaag cctctgccat tgtcgcctcg tggtttggtt
    25681 cgcgttctgg ctggcgtggg cagaacttcc cggtactcta taaccaactg ccggatcgca
    25741 atgccattgt ctttgcaacc aacgacaaac ggccggactt cctgcgcgat catccggcgg
    25801 taaaagcccc tgtgattgag atgattaacc atccgcagaa tccttacgtc aaactgctgg
    25861 tggtgtttgg tcgtgacgac aaagacctgt tgcaggcagc gaaaggtatc gctcagggta
    25921 acattctgtt ccgtggtgaa agcgtggtag tgaatgaagt gaaaccgctg ctaccgcgta
    25981 agccgtacga tgcgccgaac tgggtacgta ccgatcgtcc ggtcacattt ggcgaactga
    26041 aaacctatga agaacagtta caatccagcg gtcttgagcc agcagcgatt aacgtttcgc
    26101 taaacctgcc gccggatctc tacctgatgc gcagtaccgg cattgatatg gatattaatt
    26161 accgctacac catgccgccg gtgaaagaca gttcgcggat ggatatcagc ctgaataacc
    26221 agttcctgca atccttcaac ctgagcagca aacaggaggc gaaccgcctg ctgctgcgga
    26281 ttccggtatt acaaggtttg ctggatggca aaacagatgt ctctattccg gcgctgaaac
    26341 tgggcgcgac caaccagctg cgcttcgact ttgagtatat gaacccgatg ccgggcggtt
    26401 cggtggataa ctgtattacc ttccagccgg tgcagaatca tgtggtgatt ggtgacgact
    26461 ccaccatcga cttctcgaag tattaccact tcatcccgat gccggatcta cgcgcctttg
    26521 ctaacgcggg cttcccattc agccggatgg cggatctgtc gcaaaccatc accgtgatgc
    26581 cgaaagcgcc taacgaagca cagatggaaa cgttgctgaa tactgttggt tttatcggcg
    26641 cacagacggg cttcccggcg attaatctga cggtgaccga tgatggcagc accattcagg
    26701 gcaaagatgc cgacatcatg atcatcggtg gtatcccgga caaactgaaa gacgataagc
    26761 agatcgacct attggtgcag gcgaccgaaa gctgggtgaa aacaccgatg cgccagaccc
    26821 cgttccccgg cattgtgccg gacgagagcg atcgcgcggc agaaacccgg tcaacgctga
    26881 cctcttccgg tgcgatggcg gcggtgattg gcttccagtc gccgtataac gaccagcgca
    26941 gcgtgattgc gctgttggca gatagcccac gcggttatga aatgcttaac gatgcggtga
    27001 acgatagcgg caaacgcgcc accatgttcg gttcggtcgc ggtgatccgc gagtccggta
    27061 tcaacagcct acgtgttggc gacgtttatt acgtaggtca tctgccgtgg ttcgagcgct
    27121 tgtggtatgc gctggcaaac catccgattc tgctggcggt gctggcggct atcagtgtga
    27181 tattgctggc atgggtactg tggcgtctgc tgcgaattat tagtcgtcgt cgtcttaacc
    27241 cggataacga gtaattgaag atgaatgtgt tgcgtagtgg aatcgtgacg atgctgctgt
    27301 tggctgcctt tagtgttcag gcagcctgta cctggcctgc ctgggagcag tttaaaaagg
    27361 attacatcag tcaggaaggg cgcgtcatcg accccagcga cgcgcgcaaa atcaccacct
    27421 ccgaagggca aagttacggt atgttctttg ccctggcggc taacgaccgt gtagctttcg
    27481 ataatattct cgactggacg cagaacaatc tcgctcaggg ctctttaaaa gaacgtttgc
    27541 ccgcctggct gtggggcaag aaagagaaca gtaagtggga agtgctggac agcaattcgg
    27601 cctccgatgg tgatgtctgg atggcctggt cgttgctgga ggcggggcgt ttgtggaaag
    27661 agcagcgtta taccgacatc ggcagcgcgt tgctaaaacg tatcgcgcgg gaggaagtgg
    27721 tgacggtgcc tgggctgggt tccatgttgt taccgggcaa agtgggtttt gctgaggata
    27781 acagctggcg ttttaacccc agctacctgc cgccgacgct ggcgcagtat ttcacccgct
    27841 ttggcgcgcc gtggaccacg ctgcgcgaaa ccaatcaacg tttattgctg gaaaccgccc
    27901 cgaaaggttt ttcgccagac tgggtgcgct atgagaaaga caaaggctgg cagctaaaag
    27961 ccgaaaaaac attgatcagc agctacgacg ctatccgcgt ttacatgtgg gtaggcatga
    28021 tgcctgacag cgatccgcaa aaagcgcgga tgctcaaccg gtttaaaccg atggcgacat
    28081 tcactgagaa aaacggttat ccgccggaaa aagtggatgt ggctacgggg aaagcgcagg
    28141 gtaaaggacc ggtcggtttt tctgccgcca tgctgccctt tttacaaaac cgcgatgcgc
    28201 aggccgttca gcgccagcgc gtggccgata actttcccgg cagcgatgcc tattacaact
    28261 atgtgctgac cctgtttgga caaggctggg atcaacaccg tttccgcttc tcgacaaaag
    28321 gtgagttatt acctgactgg ggccaggaat gcgcaaattc acactaaaca tattcacgct
    28381 ttccctcggt ctggccgtca tgccgatggt cgaggcagca ccaaccgctc agcaacagtt
    28441 gctggagcaa gttcggttag gcgaagcgac ccatcgtgaa gatctggtgc aacagtcgtt
    28501 atatcggctg gaacttattg atccgaataa cccggacgtc gttgccgccc gtttccgttc
    28561 tttgttacgt cagggcgata ttgatggcgc gcaaaaacag ctcgatcggc tgtcgcagtt
    28621 agcgccgagt tcaaatgcgt ataaatcgtc gcggactacg atgctacttt ccacgccgga
    28681 tggtcgtcag gcactgcaac aggcacgatt gcaggcgacg accggtcatg cagaagaagc
    28741 tgtggcgagt tacaacaaac tgttcaacgg tgcgccgccg gaaggtgaca ttgctgtcga
    28801 gtactggagt acggtggcga aaattccggc tcgccgtggc gaagcgatta atcagttaaa
    28861 acgcatcaat gcggatgcac cgggcaatac gggcctgcaa aacaatctgg cgctattgct
    28921 gtttagtagc gatcgccgtg acgaaggttt tgccgtcctg gaacagatgg caaaatcgaa
    28981 cgccgggcgc gaaggggcct ctaaaatctg gtacgggcag attaaagaca tgcccgtcag
    29041 tgatgccagt gtgtcggcgc tgaaaaaata tctctcgatc tttagtgatg gcgatagcgt
    29101 ggcggctgcg caatcgcaac tggcagaaca gcaaaaacag ctggccgatc ctgctttccg
    29161 cgctcgtgcg caaggtttag cggcggtgga ctctggtatg gcgggtaaag ccattcccga
    29221 actacaacag gcggtgcggg cgaacccgaa agacagtgaa gctctggggg cgctgggcca
    29281 ggcgtattct cagaaaggcg atcgcgccaa tgcagtggcg aatctggaaa aagccctcgc
    29341 actggacccg cacagcagca acaacgacaa atggaacagt ctgctgaaag taaaccgcta
    29401 ctggctggcg atccagcagg gcgatgctgc gctgaaagcc aataatcctg accgggcaga
    29461 acgcctgttc cagcaggcgc gtaatgtcga taacaccgac agttatgcag tgctggggct
    29521 gggcgatgtg gcgatggcgc gaaaagatta tcccgccgcc gaacgttatt atcagcagac
    29581 cttgcgtatg gacagcggca acactaacgc cgtgcgcggg ctggcaaata tttaccgcca
    29641 gcaatcgcca gaaaaagctg aagcgtttat cgcctcgctc tctgccagtc agcggcgtag
    29701 cattgatgat atcgaacgca gcctgcaaaa cgaccgtctg gcacagcagg cagaggcact
    29761 ggaaaaccag ggcaaatggg cgcaggcggc agcacttcag cggcaacgac tggcgctgga
    29821 ccccggcagc gtatggatta cttaccgact ttcgcaggat ctctggcagg ccggacaacg
    29881 cagccaggcc gatacgttaa tgcgcaatct ggcgcagcag aagccgaacg acccggagca
    29941 ggtttacgct tacgggctgt acctctctgg tcatgaccag gacagagcgg cgctggcgca
    30001 tatcaacagc ctgccgcgcg cgcagtggaa cagcaatatt caggagctgg ttaatcgact
    30061 gcaaagcgat caggtgctgg aaactgctaa tcgcctgcga gaaagcggca aagaagctga
    30121 agcggaagcg atgctgcgtc agcaaccacc ttccacgcgt attgacctca cgctggctga
    30181 ctgggcgcag cagcgacgtg attacaccgc cgcccgcgct gcatatcaga atgtcctgac
    30241 gcgggagcca actaacgccg atgccattct ggggctgacg gaagtggata ttgctgccgg
    30301 tgacacagcg gcggcacgta gccagctggc gaaactgccc gctaccgata acgcctcgct
    30361 gaacacacag cggcgcgtgg cgctggcgca ggcgcagctt ggagataccg cagcggcgca
    30421 gcagacgttt aataagttga tcccgcaggc aaaatctcag ccaccgtcga tggaaagcgc
    30481 gatggtgctg cgcgacggtg cgaagtttga agcgcaggcg ggcgatccaa agcaggcgct
    30541 ggaaacctac aaagacgcca tggtcgcatc cggtgtgact acgacgcgtc cgcaggataa
    30601 cgacacattc acccgactga cccgtaacga cgagaaagat gactggctga aacgcggcgt
    30661 gcgcagcgat gcggcggacc tctatcgcca gcaggatctt aacgtcaccc ttgagcacga
    30721 ttactggggt tcgagcggca ccggtggtta ctccgatctg aaagcgcaca ctaccatgtt
    30781 gcaggtggat gcgccgtatt ctgacgggcg gatgttcttt cgcagtgatt tcgtcaatat
    30841 gaacgtcggc agtttctcca ctaatgccga tggcaaatgg gatgacaact ggggcacctg
    30901 tacattacag gactgtagcg gcaaccgcag ccagtcggat tccggtgcca gcgtggcggt
    30961 cggctggcga aatgacgtct ggagctggga tatcggtacc acgccgatgg gcttcaacgt
    31021 ggtggatgtg gtcggcggca tcagttacag cgatgatatc gggccgctgg gttacaccgt
    31081 taacgcccac cgtcggccca tctccagttc tttgctggcc tttggtgggc aaaaagactc
    31141 cccgagcaat accgggaaaa aatggggtgg cgtacgtgcc gacggtgtgg ggctaagtct
    31201 gagctacgat aaaggtgaag caaacggcgt ctgggcatcg cttagtggcg accagttaac
    31261 cggtaaaaat gtcgaagata actggcgcgt gcgctggatg acgggctatt actataaggt
    31321 cattaaccag aacaatcgcc gcgtcacaat cggcctgaac aacatgatct ggcattacga
    31381 caaagatctg agtggctact cactcggtca gggcggttac tacagtccgc aggaatacct
    31441 gtcgtttgcc ataccggtga tgtggcggga gcgcacggaa aactggtcgt gggagctggg
    31501 ggcgtctggc tcgtggtcgc attcacgcac caaaaccatg ccgcgttatc cgctgatgaa
    31561 tctgatcccg accgactggc aggaagaagc tgcgcggcaa tccaacgatg gcggcagcag
    31621 tcagggcttc ggctacacgg cgcgggcatt acttgaacga cgtgttactt ccaactggtt
    31681 tgttggcacg gcaattgata tccagcaggc gaaagattac gcacccagcc atttcctgct
    31741 ctacgtacgt tattccgccg ccggatggca gggtgacatg gatttaccgc cgcagccgct
    31801 gataccttac gccgactggt aagttttcag atagcgcctc tcttaatgcc gctgcgatcg
    31861 ggtatactcg ggcggcaatc tgggatttcc ggggggagac aatttgcgcg taagtcgctc
    31921 gttaacaatc aagcagatgg caatggtggc agccgttgtc ctggtgttcg tttttatttt
    31981 ttgcaccgtt ttgctgttcc atctggtcca acagaatcgc tataacacgg ctacgcaact
    32041 ggaaagcatt gctcgctctg tccgcgaacc cttatcttca gctattttga aaggcgatat
    32101 tcccgaagcg gaagctattc ttgccagcat taaaccggca ggcgtggtca gccgtgccga
    32161 tgtagtgctg cctaaccagt tccaggcgct gcgtaaaagt tttattccag agcgcccggt
    32221 gccggtaatg gttactcgcc tgtttgagct accggttcaa atctcgctgg gcgtttactc
    32281 gctcgaacgt ccggcaaacc cgcagccaat tgcctatctg gtactacagg cggattcctt
    32341 ccgtatgtat aagttcgtga tgagcaccct ctcaacgtta gtgaccattt acttactttt
    32401 gtcgcttatc ctgaccgtcg ccatcagctg gtgcattaac cgcctgattt tgcatccgtt
    32461 acgcaatatt gctcgcgaac ttaacgccat cccagcccag gagcttgttg gtcaccaact
    32521 ggcattaccg cgtctgcatc aggacgatga aatcggtatg ttggtgcgca gttacaacct
    32581 caaccagcaa ttgctgcagc gccattatga agaacagaac gaaaatgcga tgcgcttccc
    32641 ggtgtcggat ttgccgaaca aagccttgct gatggagatg ctggagcagg ttgtcgcgcg
    32701 taaacaaacc accgcgctga tgatcatcac ctgtgaaacc ctgcgtgata ctgcgggcgt
    32761 gctgaaagag gcgcaacgag aaattctgct gctgacgctg gtggaaaaac tcaaatcggt
    32821 actgtcgcca cgtatgatcc tcgcgcagat tagcggttat gactttgctg tcattgccaa
    32881 cggtgtacag gaaccgtggc acgcaatcac cttaggtcag caagtgctca ctatcatgag
    32941 cgagcgcctg ccgattgaac gtattcaact ccgtccgcac tgtagcattg gcgtggcgat
    33001 gttctacggc gatctcaccg ccgaacagct ttacagtcgc gctatttctg cggcatttac
    33061 cgctcgccat aaaggcaaga atcagattca gttctttgat ccgcagcaga tggaagccgc
    33121 ccagaagcgg ttgacggaag agagcgatat ccttaatgca ctggaaaatc atcagtttgc
    33181 aatttggtta cagccacagg tcgagatgac cagcggtaaa ctggtcagtg cggaagtgtt
    33241 actgcgtatc cagcaaccgg atggcagttg ggacctgccg gatggcttaa tcgatcgcat
    33301 tgagtgctgt gggctcatgg ttaccgtcgg tcactgggtg ctggaagagt cctgtcgatt
    33361 gcttgcagcc tggcaagagc gcggcattat gctgcccttg tcggtaaacc tctctgcgct
    33421 gcaactgatg cacccgaata tggtggcgga tatgctggaa ctgttaaccc gctatcgcat
    33481 tcagccggga acactgattc tggaagtgac agaaagccga cgtattgacg accctcatgc
    33541 tgcggtggca atcctccgtc cgctgcgcaa tgccggagtt cgggtggcgc tggatgattt
    33601 cggcatgggc tacgcagggc tgcgtcagct gcagcatatg aaatcgttgc caatcgacgt
    33661 actgaaaatc gacaaaatgt ttgttgaagg cttgccggaa gatagcagca tgattgctgc
    33721 aattatcatg ctggcgcaga gcctgaactt acaaatgatt gccgaaggcg tggagactga
    33781 agcacaacgc gactggctgg caaaagcggg cgttggtatt gcccagggct tcctttttgc
    33841 tcgcccactc cctattgaaa tcttcgaaga gagttacctg gaagaaaagt agctacccca
    33901 aactgattac aaaactttaa aaagtgctgg tttgtgcgag ccagctcaaa ctttttaacc
    33961 tttttgtttc aattatgatc caggtacatt tctgtgatgt tgtctgggtg ttattttaag
    34021 gccgcaggta ccccataacc ttacaagacc tgtggtttta ctaaaggaca ccctatgaaa
    34081 acctctctgt ttaaaagcct ttactttcag gtcctgacag cgatagccat tggtattctc
    34141 cttggccatt tctatcctga aataggcgag caaatgaaac cgcttggcga cggcttcgtt
    34201 aagctcatta agatgatcat cgctcctgtc atcttttgta ccgtcgtaac gggcattgcg
    34261 ggcatggaaa gcatgaaggc ggtcggtcgt accggcgcag tcgcactgct ttactttgaa
    34321 attgtcagta ccatcgcgct gattattggt cttatcatcg ttaacgtcgt gcagcctggt
    34381 gccggaatga acgtcgatcc ggcaacgctt gatgcgaaag cggtagcggt ttacgccgat
    34441 caggcgaaag accagggcat tgtcgccttc attatggatg tcatcccggc gagcgtcatt
    34501 ggcgcatttg ccagcggtaa cattctgcag gtgctgctgt ttgccgtact gtttggtttt
    34561 gcgctccacc gtctgggcag caaaggccaa ctgattttta acgtcatcga aagtttctcg
    34621 caggtcatct tcggcatcat caatatgatc atgcgtctgg cgcctattgg tgcgttcggg
    34681 gcaatggcgt ttaccatcgg taaatacggc gtcggcacac tggtgcaact ggggcagctg
    34741 attatctgtt tctacattac ctgtatcctg tttgtggtgc tggtattggg ttcaatcgct
    34801 aaagcgactg gtttcagtat cttcaaattt atccgctaca tccgtgaaga actgctgatt
    34861 gtactgggga cttcatcttc cgagtcggcg ctgccgcgta tgctcgacaa gatggagaaa
    34921 ctcggctgcc gcaaatcggt ggtggggctg gtcatcccga caggctactc gtttaacctt
    34981 gatggcacat cgatatacct gacaatggcg gcggtgttta tcgcccaggc cactaacagt
    35041 cagatggata tcgtccacca aatcacgctg ttaatcgtgt tgctgctttc ttctaaaggg
    35101 gcggcagggg taacgggtag tggctttatc gtgctggcgg cgacgctctc tgcggtgggc
    35161 catttgccgg tagcgggtct ggcgctgatc ctcggtatcg accgctttat gtcagaagct
    35221 cgtgcgctga ctaacctggt cggtaacggc gtagcgacca ttgtcgttgc taagtgggtg
    35281 aaagaactgg accacaaaaa actggacgat gtgctgaata atcgtgcgcc ggatggcaaa
    35341 acgcacgaat tatcctctta atctcatcac ttatgcccgc actcccttcc ccgagtgcgg
    35401 gtgtttgcgc ataattgacc cctgtgcctg aacttaccac ctgggtgagt tcaggtttat
    35461 ttcacttctt ccgtgacatt ttcatgttct tgcggtctaa cacgaagtgt ttttaacgtc
    35521 atttagactg accgacaacg ggcagttttt ttattaccag gatagttgat caggggttca
    35581 catgcagggc acaaaaattc gacttttagc gggcggtttg ctgatgatgg ccactgctgg
    35641 ctatgtgcag gcagatgcgc tccagcctga tccagcatgg caacagggga cgctttccaa
    35701 cggtttacag tggcaagtgc tgaccacccc ccagcgtccc agcgatcgtg ttgaaattcg
    35761 cctgctggtt aataccggtt cgctcgccga aagtacacaa cagagcggtt acagtcacgc
    35821 catccctcgt attgcgctaa cgcaaagcgg tggccttgac gcagcacagg cgcgttcatt
    35881 gtggcagcag gggatcgacc ctaaacgccc gatgccgccg gtaattgtct cttatgacac
    35941 cacgctgttt aatctgagtt tgcccaataa ccgtaacgat ttgctgaaag aagcgctctc
    36001 ttatctggca aatgccactg gcaaattgac catcacacca gaaaccatca accacgcgct
    36061 gcaaagtcag gacatggtgg caacctggcc tgccgatact aaagagggct ggtggcgcta
    36121 tcgtctgaaa gggtcaacct tgttaggtca cgatcctgcc gatccgctga aacaacccgt
    36181 tgaagcggaa aaaattaaag atttctatca gaaatggtac accccggatg caatgacgct
    36241 actggtggtg ggaaacgtgg atgcgcgctc ggttgtcgac caaataaaca aaacgtttgg
    36301 cgaactgaaa ggcaaacgtg aaacgccagc tccggtgccg acgctttctc cgctgcgtgc
    36361 ggaagcggtg agtattatga ctgacgcggt gcgtcaggac cggttatcta tcatgtggga
    36421 tacgccgtgg cagccgattc gtgaatcagc cgcactgctg cgctactggc gtgcggacct
    36481 ggcccgcgag gcgctgttct ggcatgttca gcaagcgtta agcgccagta acagcaaaga
    36541 catcggtctt ggatttgact gccgtgtgct gtatctgcgt gcgcagtgtg ccatcaacat
    36601 cgaatcacca aacgacaagc tgaacagcaa ccttaatctg gtggcgcgtg aactggcgaa
    36661 ggttcgcgat aaaggtctgc cggaagaaga gttcaatgcg ttagtggcgc aaaagaaact
    36721 ggagctgcag aaactgtttg ccgcctatgc gcgggctgat accgatattc tgatgggtca
    36781 gcggatgcgt tcgttgcaaa atcaggtcgt cgatatcgcg ccggagcagt atcagaaact
    36841 gcggcaggat ttccttaaca gcctgacggt ggagatgtta aatcaggatc tgcgtcagca
    36901 gttgtcgaat gatatggcgt taatactgct gcagccgaaa ggcgagccgg aatttaacat
    36961 gaaagcgttg caggcggcct gggatcaaat catggcccca tcgactgctg ctgccgccac
    37021 ctctgtcgcc acggatgacg tacatcctga agtgacggat attccacctg cacagtaatg
    37081 cctgatggtg atgagttagc acaaccatca ggtatttatg gatgagctgg atagaggagt
    37141 aacactttat ccagcctttt gcatatgctg cgtttacgct ggcatcgcct cacgcgggat
    37201 aatcgcgccg cgatactgaa taacggtact tgcggtcagg tgcccgcgtt tcgccgcgtt
    37261 ttccgcactg ccgcccgtca aacgtaccgc cagataaccg gcgctgaaag agtcgccagc
    37321 tgcggtggta tcgatcactt tttctttcgg cagtttcacc gccggaacat ccactaaccc
    37381 ttcgccagca atggacacca ggcaagaatc cgccccgcgt ttcaccacca cttctttcac
    37441 gcccgcgtta tgggtgcgcg caatgacgtc ttccaccggc tgttgacccc acagcgcgtc
    37501 ttcatcgtcc agcgtcagga aggcgatatc cgtgcattcc agcatttgtt ggtacacctg
    37561 ctgtgtctct tctttgctgg cccacaggcg cggacgatag ttattgtcga aaatcacttt
    37621 tccgccgttg gcgcggcatt cgcgcagcag ggaaagcagc ttttcgcggc tggtcgggct
    37681 taagatcgcc aggctaatcc cgctcaggta gagataatcg aaattcgcca gctcttcgca
    37741 aatcgccgca gactgctcac tctccagcca gaatttggcg gcggcttcgt tccgccagta
    37801 gtagaacgta cgctcgccgg tgctgtcggt ttcaatgtag taaaggcccg gcagacggtt
    37861 ttccatccgt tgggtcaggg aagtatcaac gttctcgccg tgccaggcgt ccagcatttg
    37921 ctggctaaaa ctgtccgttc ccagcgccgt tacgtaatga acggttaatg ccgcaggatc
    37981 gacctgacgg gcgatataga cggaagtgtt cagggtatcg ccgccgaaac cgcgcttaac
    38041 gtccgcgcct ttctcggaaa gctcaatcat gcattcgcca atcacggcaa tctttttgga
    38101 catagtcgtg aacctgatct gtaaaaaatt agcgttagtt tgcgctgtgg tggtttgctg
    38161 gtcaaccata ttaaaacagt gttccattat ttttttgagc cagaacgtat tcctgaaaga
    38221 ttccgttgtg gagtgaggaa atttaccccg gccaggcggt aaagttctgc ccttgcgcgc
    38281 cgataatctt tgtcgagtcc gggcagcatc acttttaaac acaggacatc tttgatgata
    38341 aggcaggtta tccagcgaat aagcaaccct gaagcaagca tcgagagctt gcaggaacgg
    38401 cgtttttggt tgcagtgtga gcgtgcttac acctggcagc cgatctatca aacatgcgga
    38461 cggttaatgg ccgtggagct attaacggtg gtcacgcatc ccttgaaccc ttcgcaacgc
    38521 ctgccgccgg atcgctattt tactgaaatc accgtcagcc atcggatgga ggttgtgaaa
    38581 gagcagattg atttgctggc gcaaaaagcc gacttcttta tagagcacgg cctgctggca
    38641 tcggtcaata ttgatggccc tacgctcatc gccctgcgtc agcaaccaaa aatcctgcgc
    38701 cagattgagc gtcttccctg gctgcgtttc gaactggtgg agcatatccg tctgccgaaa
    38761 gattcaacct ttgcctcgat gtgtgaattt ggcccgctgt ggctggatga ttttggcacc
    38821 gggatggcaa atttctctgc gctaagtgaa gtgcgttatg actacatcaa aatcgcgcga
    38881 gagctgttcg tgatgctgcg tcagtcgccg gaaggccgca cactcttttc tcagctttta
    38941 catctaatga atcgctattg tcgcggggtg attgtcgagg gcgtagaaac accggaagag
    39001 tggcgtgatg ttcagaactc gcccgcattc gccgcacaag gctggtttct ttcacgcccg
    39061 gcaccgatag aaacgctgaa tacggcggtt ctggcgctat aagctgcctc attttccgcc
    39121 tggctggact atctttagga ctggcacagg aagaatatga ggaagcgaac gatgagcaag
    39181 gcaggcaaaa taaccgctgc gatttcaggg gctttcttgt tgttgattgt cgtggcgatc
    39241 attttgattg caacttttga ctggaatcga ctcaaaccga ccatcaacca gaaagtctct
    39301 gcggagttga atcgtccgtt cgctatccgt ggcgatctgg gcgtggtgtg ggagcggcaa
    39361 aaacaagaaa ctggctggcg cagctgggtg ccgtggcccc atgtacacgc ggaagacatc
    39421 attcttggca atccaccgga tattcccgaa gtcacgatgg tgcatttgcc acgcgtagag
    39481 gcaacgctgg ccccgctggc gctgctgacc aaaacggtct ggctgccgtg gatcaagctc
    39541 gaaaagcccg acgcgcgcct gattcgcctc tccgaaaaga acaataactg gacgtttaat
    39601 ctcgccaacg atgataacaa agacgcgaat gcaaagccgt cggcatggtc gtttcggctg
    39661 gataatattc ttttcgatca agggcggatc gctattgatg acaaagtaag caaagcggat
    39721 ctggagattt ttgttgatcc gttaggcaag ccgctgccgt tcagcgaagt tactggatcg
    39781 aaaggtaaag cggataaaga aaaggtgggc gattacgttt ttggcctgaa ggcgcaggga
    39841 cgttataacg gtgaaccgct cacgggtacg ggaaaaatag gcggtatgct ggcgctgcgt
    39901 ggcgaaggga cgccgtttcc ggtacaggct gatttccgct ctggtaacac ccgtgttgct
    39961 tttgatggcg tcgtgaatga cccaatgaag atgggcggtg tcgatttacg gcttaaattt
    40021 tctggcgatt cactgggtga tctctatgaa ctgacgggcg ttctgctgcc cgataccccg
    40081 ccgtttgaaa cggatggtcg gctggtagcg aaaatcgaca ctgaaaaatc gtcggtcttt
    40141 gattatcgcg gttttaatgg gcgaattggc gatagcgata tccacggttc tctggtctac
    40201 accaccggaa agccacgacc aaaactggaa ggtgatgtcg agtcgcggca attgcggctg
    40261 gcggacctgg gaccgttaat tggcgttgac tccgggaaag gggcagaaaa gtcgaaacgg
    40321 tctgaacaga agaagggcga aaaaagcgtt cagcctgcgg gcaaagtgct gccttatgac
    40381 cgcttcgaaa ccgataaatg ggacgttatg gatgccgatg ttcgcttcaa agggcggcgc
    40441 attgagcatg gcagtagcct gccgattagc gatctttcta ctcatatcat cctcaaaaat
    40501 gctgacctgc gcctgcaacc gctgaaattt ggcatggcgg gcggcagcat tgcggcgaat
    40561 attcatctgg aaggcgataa aaagccgatg caggggcggg cagatattca ggctcgtcga
    40621 ctgaaactga aagaactgat gcccgatgtg gaactgatgc agaagacgct gggggaaatg
    40681 aacggtgacg cggaactacg cggtagcggt aactcggtgg cagcactttt aggcaacagt
    40741 aacggcaacc tgaaactgtt gatgaatgac gggctggtga gccgcaacct gatggagatt
    40801 gttgggctga atgtcggcaa ctacattgtc ggtgcgatat ttggtgacga tgaggtgcgg
    40861 gtgaactgcg cggcggcgaa tctgaatatt gccaacggcg tggcgcgccc gcagattttt
    40921 gctttcgata ctgagaacgc gttgattaat gttaccggca cggcaagttt tgcttcggaa
    40981 cagctggatt tgactattga tccggagagt aaagggattc ggattatcac actgcgttcg
    41041 ccgctgtatg tgcgtgggac gtttaaaaat ccgcaggctg gggtgaaagc cgggccgttg
    41101 attgcccgtg gtgctgttgc tgcggcactg gcaacgctgg taacaccggc ggcggcgtta
    41161 ctggcactga tctcaccttc cgaaggggag gctaatcagt gtcggacgat attgtcgcag
    41221 atgaagaagt gatgaggttg gattgtgttt gctggtgagt tcgggagcag c
//
LOCUS       DAZFXT010000030        40905 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.030, whole genome shotgun sequence.
ACCESSION   DAZFXT010000030 DAZFXT010000000
VERSION     DAZFXT010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40905
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.030"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(125..1129)
                     /gene="argF"
                     /locus_tag="ACHKW0_001700"
     CDS             complement(125..1129)
                     /gene="argF"
                     /locus_tag="ACHKW0_001700"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HFO7474549.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1291..1707
                     /gene="rraB"
                     /locus_tag="ACHKW0_001701"
     CDS             1291..1707
                     /gene="rraB"
                     /locus_tag="ACHKW0_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HFO7474550.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1753..2256)
                     /gene="yjgM"
                     /locus_tag="ACHKW0_001702"
     CDS             complement(1753..2256)
                     /gene="yjgM"
                     /locus_tag="ACHKW0_001702"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7474551.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSQSDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            2449..3645
                     /gene="yjgN"
                     /locus_tag="ACHKW0_001703"
     CDS             2449..3645
                     /gene="yjgN"
                     /locus_tag="ACHKW0_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjgN family protein"
                     /protein_id="HFO7474552.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
     gene            complement(3701..6556)
                     /gene="valS"
                     /locus_tag="ACHKW0_001704"
     CDS             complement(3701..6556)
                     /gene="valS"
                     /locus_tag="ACHKW0_001704"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HFO7474553.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(6556..6999)
                     /gene="holC"
                     /locus_tag="ACHKW0_001705"
     CDS             complement(6556..6999)
                     /gene="holC"
                     /locus_tag="ACHKW0_001705"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HFO7474554.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            complement(7133..8644)
                     /gene="pepA"
                     /locus_tag="ACHKW0_001706"
     CDS             complement(7133..8644)
                     /gene="pepA"
                     /locus_tag="ACHKW0_001706"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HFO7474555.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8798..8848
                     /gene="ytgA"
                     /locus_tag="ACHKW0_001707"
     CDS             8798..8848
                     /gene="ytgA"
                     /locus_tag="ACHKW0_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YtgA"
                     /protein_id="HFO7474556.1"
                     /translation="MSIIILATISTTQENK"
     gene            8911..10011
                     /gene="lptF"
                     /locus_tag="ACHKW0_001708"
     CDS             8911..10011
                     /gene="lptF"
                     /locus_tag="ACHKW0_001708"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7474557.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            10011..11093
                     /gene="lptG"
                     /locus_tag="ACHKW0_001709"
     CDS             10011..11093
                     /gene="lptG"
                     /locus_tag="ACHKW0_001709"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7474558.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(11212..12714)
                     /gene="yjgR"
                     /locus_tag="ACHKW0_001710"
     CDS             complement(11212..12714)
                     /gene="yjgR"
                     /locus_tag="ACHKW0_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like C-terminal domain-containing
                     protein"
                     /protein_id="HFO7474559.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHSVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDDVDRESAYEMLQKGVQASTEQQKNPPTKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(12844..13863)
                     /gene="ahr"
                     /locus_tag="ACHKW0_001711"
     CDS             complement(12844..13863)
                     /gene="ahr"
                     /locus_tag="ACHKW0_001711"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HFO7474560.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            14059..14143
                     /locus_tag="ACHKW0_001712"
     tRNA            14059..14143
                     /locus_tag="ACHKW0_001712"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14093..14095,aa:Leu,seq:caa)
     gene            14330..15574
                     /locus_tag="ACHKW0_001713"
     CDS             14330..15574
                     /locus_tag="ACHKW0_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7474561.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGNGMHLLVHPNGSKYWRLQYRF
                     GGKQKMLALGVYPDVSLADARARRDDARKLLANGIDPGDKKKNDKIEQEEARTFEQLA
                     IEWHATNKKWSEEHSRRVLKSLEDNLFPAIGKRNIADLKTRDLLAPIKAVELSGRLEV
                     ASRLQQRTTAIMRYAVQSGLLDYNPAQEMVGAVASNNRQHRPALQLKLIPELLRRIDS
                     YTGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETSMWTIPAEREAIEGVKHSQRGS
                     KMRTPHLVPLSHQALAILKDVYKLSGDRDFVFIGDHDHRKPMSENTVNKALRVMGYDT
                     KVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHMERSSVRAAYIHKAEHLDERRLML
                     QWWADFLDANRESEVSPYDFGK"
     gene            16114..16965
                     /locus_tag="ACHKW0_001714"
     CDS             16114..16965
                     /locus_tag="ACHKW0_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001446456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474562.1"
                     /translation="MLVNSWCNALNEAISTSATVCAEDLYKGGHWATAKAILDDYPID
                     LWILSAGLGLLHHKDNVVPYKATFAVGYDESIPLYSQEYVGKSFHRTWWKEITNRSIF
                     KSKHPTSIVELMKKRKRDYYIICGSPDYINAIELDIINGLEYLVDAKKQLLIITSKKI
                     NGRLTTYLFKTNQNMAQWLRCNMLMLNISVAKYIVKEFTSKQLNDLNELSQKLKEELK
                     ELPEREVKKGIRRSPEEVKSFILKIMEKNPGISATHALREFRDSGNSFEEKRFRAEFM
                     ALREAKP"
     gene            17039..18289
                     /gene="dpdA"
                     /locus_tag="ACHKW0_001715"
     CDS             17039..18289
                     /gene="dpdA"
                     /locus_tag="ACHKW0_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase DpdA"
                     /protein_id="HFO7474563.1"
                     /translation="MSKLKYFFPDSQDFIDPSFDFFRETRNEHRVRQRDDQYPHEVFP
                     HPYDGMLVSKAVVDGLGGGESKYTRAQRLRYFRNGMKHFFRLPENMETMGDCGAFTYV
                     NQDVPPYRVEEVIEFYETSRFNHGVSLDHIVFGYEKPGEVFSGEVLTECRRRQDITLT
                     LAQEFLNKSQKSCFTPFGVAHGWSKTSYRQSVEALLAMGYKNITMGGMVPLKTAQILE
                     TLEEIKPLLKSDTRVHLLGIARPESFSDFIKFGVTSIDSTTPLQQAFKDRKNNYHTLD
                     GPAYTAVRVPQFDANPSLSRKIKSGVIDQDIARHLEKDAMNALFEYDKGALSLEKALD
                     AVLAYERLHSGEKEADKIRADYERTLGERPWKQCQCNICQAIGINVIIFRGAERNRRR
                     GFHNIQVLYNRLQHTLSLRSEELS"
     gene            18286..19416
                     /gene="dbpB"
                     /locus_tag="ACHKW0_001716"
     CDS             18286..19416
                     /gene="dbpB"
                     /locus_tag="ACHKW0_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DGQHR domain-containing protein DpdB"
                     /protein_id="HFO7474564.1"
                     /translation="MSEYRVPALRIRQGEERQLYSLAIEGKQISKIAAISRIRRGEES
                     LVGYQRPEVRNHIREIQRYIESANPMIPNPVIIAFDKRVRFEPLTDNDDMGHLVIPFS
                     EDKDFEKPGFIVDGQQRTAALRDAEINSFMMPVSAFIANDAEEQREQFMLVNSTKPLP
                     KTLLYELAPHTHGRLPSDLQMRKFPSLLTQRLNFGEGSLAGRIKTATNPDGVIADNSM
                     IKMIDASLREGALYRFRDPATGLGDEEKMVKLLNNFWSAVETVFTDDWDKKPRYSRLL
                     HGVGILALGSLMDEIDQVHQDYKGEPGWTEIPSYTRFVEELNRIKPLCAWSSGVWNFG
                     TDIDGQPIVRKWNELQNLSKDISLVTDYLVTGYMKTVNADIK"
     gene            19853..23008
                     /gene="dpdE"
                     /locus_tag="ACHKW0_001717"
     CDS             19853..23008
                     /gene="dpdE"
                     /locus_tag="ACHKW0_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001127873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdE"
                     /protein_id="HFO7474565.1"
                     /translation="MPLEVGDLVDSPYGIGKLIRINNNDALIKYFDSPVNYEGIEQTV
                     EVKHLKKAELHAESLVYRYDYEVAHWQIARVVGAVPGGVRLHFPNKQQEDVSLNDIFV
                     RWDRPISDAAALLAERITYTPFWQDARLAYQHQMLKQRNACAGITSSLSSSIELEPYQ
                     LAVARRVLSDPIQRYLLADEVGLGKTIEAGLIVRQHVTENPLAHCVVIVAPGVLHRQW
                     QQELSARFHLEELLNKSIHIIDMAAFSTADPLSFRPDMLVIDEAHQVGAWAWDAEYEQ
                     RYASVNYLAQQSEKLLLLSATPVTGNEKNFLAMLHLLDAKNYPLTDTGLQKFQQRIQQ
                     REEIGSLFYAFNCDNDNMTLEENLEALTRMFPEDEVLNGMASQLLPHLDMMQPLRSEA
                     RDLLINKIRHYIGNRYRLHHRLLRNRRSNPAISVLMPGLSGLSLKKYATTNASNDMMI
                     AWRESAIMMHPQDPTLAAIYQLLFEAAIATPELLARLANYRLQQESLGAAQGFLYSDS
                     QLALLQHPLFDGEKEILLELIDRCKMECDSKRQRLIDVLEKLLDSNVRELPYIVVFCD
                     QPWVADQVFTDLMIPFIGKIQRHNPDKEVRFGKGSKCQILVCDRRAEEGVNLHGGRKI
                     ALHYDLLLSPNRMEQRLGRLNRYCATESATTILSMALVSEAEGLQNQWLTILNEVFDV
                     FEESIASLQYLIDEELQRLISNLYPLGEESMMQLSARLAGEDGRLTVEKQRIEMQEVL
                     LQIDGDIIEAREYADALAEIDENEEEFRRCARNLIGNVLKFDFIPDEKDPSIFRYRFN
                     PGDRGTGTLVSPQRLQKHCLLGFDLNQSSGSSPMTWPLSYYRADTSQGKIRPARLGEP
                     FHDAILQMLDFEERGVASAIIRITPGMGDENIFFRYDFLIEADVHTPALQRLADHLMP
                     PETITLWLDQAGQQVTNAEVLMILNEDYKPKDKGGRHLNLNEERWAQISHCISAKDWR
                     TWCNDSYSIAQRLAVEQFATQQALSLSRLEHYLSSAALHHANRQETGQMLRQGIAQPK
                     MTCLATRALIIASEAILDEDS"
     gene            23014..25581
                     /gene="dpdF"
                     /locus_tag="ACHKW0_001718"
     CDS             23014..25581
                     /gene="dpdF"
                     /locus_tag="ACHKW0_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdF"
                     /protein_id="HFO7474566.1"
                     /translation="MNLAQLIDNDFISSRLSECLLPEATAGHLDRRALIRDWLYREKH
                     AHHNDNELTVPDDSQPGWPRQEEWEASGINCQRLGQTFLLSAGPAWCPQWLEQGNGMP
                     FLASDQHQFCRQDTRVPMDYVLRRYFKKDNYISYQAPGQRDAVRRALFAPPGSTLLVN
                     LPTGTGKTLVAQALALTASAPGALCVLIAPTTALVLELASRFRKLLIEAGNKAESVTG
                     WYSGLPAQDKYDTYMRIRVGEQRLIVTSPEATCSSLQFALFEAAKQGGLRHVIVDEAH
                     IVASWGNDFRPHFQQLGGLIRGLKRVSREANLTPCSTLLMSATITEASRKILRDTFDQ
                     EMSELHACHLRPEPAYWCYRAKDHSDKRDKVLQSLAHAPRPLILYVTRPKEAEEWAQQ
                     LRLQGYQRLATFTGDTPSEVRRSLLEKWDKNQLDIMIATSAFGVGMDKNDVRTVIHAT
                     VPENMDRFYQEVGRGGRDGYACTSLLIYNHQDVKQAEHLSSQKLIGLELGLERWKKLW
                     GSAESVGDNLYRIDIERIHSGLNFRSKQNKLWNLRTVLMMVRAGVLELDADLQNPPDK
                     KDFADVNRYEAAKEQIIRQNGNSLVVRILRSHHTGEELWKTRIIPSREETLQAARANH
                     NALINWLHTPLSRPLCEALVDLYSMAEFIPGHACGGCPACRDNQALTSNYPSPPTLLL
                     NPPASVAQQALSRWQALSGLSYSSCFITYPPVNDDEWERENWYHRIATLLVQLYHRGI
                     VQRVWADPALLHSIFSAIPRGERLILLSDELPARDEPAPDNDVMAELAELVLPTQESC
                     ANGYLPPFNPAPLQLTLIPHDMPDPHHPGGAHYVLLDNHNWITIEQLQRKLDSVDYQ"
     gene            25565..26470
                     /gene="dpdG"
                     /locus_tag="ACHKW0_001719"
     CDS             25565..26470
                     /gene="dpdG"
                     /locus_tag="ACHKW0_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdG"
                     /protein_id="HFO7474567.1"
                     /translation="MSIINNAHGGSQYAVLAAFYQVLKASPKNQLPREKLMALCAPET
                     LQTGDSQNARGKASQELRAWLDLGLLTEEKVEETTQISFNANYFSTKSMPLRQAARRC
                     LQAQENNSDLSSRDERAVDLTLLAALLLALDVYQNAEIKSGNLEVLVNTFIPDFRINS
                     NEIAVVPGYLSWLGFAQQLTRDSYAIDPTDSIREELPFLMRVGEQLSIGEVLQRLNRA
                     LPVLDGGSYRQQVEERISLYGWHSLSPNRLSTSLSRALLRLQLSGVINMKAESDASGA
                     MQLTGRNGSVLRTVTHLTLNDVGVH"
     gene            26470..29526
                     /gene="dpdH"
                     /locus_tag="ACHKW0_001720"
     CDS             26470..29526
                     /gene="dpdH"
                     /locus_tag="ACHKW0_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdH"
                     /protein_id="HFO7474568.1"
                     /translation="MPQLQSYWPSEQNVTACILTEAESLADSQLLAVHEPMRLDRIEF
                     QSGKVTQVGEGALLDFLLEHNRPLPLIGASGVGKSHLVRWVHAQLKRREDHAKYHIIR
                     IPKNASLPRVLAAILEGLDGEEYQSIREKVNGVGKQLIPENVAEHIALKLRQALNAAY
                     TDAIEELRQEKMGKIQLEESRKKQLEEIQQHAASSRLPALFFDSVMTQYFTAPGACLH
                     NIAQRFCQGANDDSIANLRYEMSAEDFTFKGLDFRQVSPTVLPYLRNQQLLTSDEKKQ
                     AAARVVNVVRSQALEDTFVELFSFNRANFQDLMRMIRRQLLAEGRDLILLVEDLTATS
                     AIEDVLIACLIEEDEYDGKKVLCTLRSIIAVTEGHDSFKRLRNTLGTRARYEWVIQQH
                     ASENDVALKKRVIDFCGRYLNAARHGAAALETYHLQQDAQCYQEIPVWQDQEVLENES
                     AAPALASFGSSSIGHPLFPFNPAAIGQLVERHCKVKEQGLVFIPRNILHEILREPLKK
                     YRQNYLDGQFPPSQFAEIVCNPALQQRVRLEGLSQPERINSLLAVWGGNSTNLAGLTP
                     AICKEFGLPDAAVLLGGGGTPPEPAPEPVPEPAPVPESVPAPPSPLDEWKLVLDKWHQ
                     SKTLEQTRARDIRKWLLEALNARIDWSTELIQTSLMASSAKTQGRIHLPGVLVGNQSH
                     PLVKLEDTPELRSACLAMAHFNHYQSWDYDGSEIDYAAFHSFMDCIEHEVKLKVLQEE
                     REELSSIINDLQLSGAFLGLDGLSASHPATLVQALLTEPQVANSNHPLLDEFLTQARD
                     TVIDEHLKRRERLIALTAAKKPGAQPYAINGLMLVEASKKQSPDAGKQPGKIHEVKSK
                     LGGYRTLLLKTQESITERLSDGNSASLAAVNLCDTMKEIVELANSADVARPMGETVPV
                     LLKLLKSWKDPELKKLIRQLEKLTLPADNSHMLMREILAINGEQFEAFRQLLENWGKF
                     EDATDKYVQQRISDKGGDQVEDAENQVTDTLANLAEQLTLLEADLS"
     gene            29523..30314
                     /locus_tag="ACHKW0_001721"
     CDS             29523..30314
                     /locus_tag="ACHKW0_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474569.1"
                     /translation="MSEVNINDVVTPVASVPEERGIFARRSGDFKARLQRYQQMEKAQ
                     DIRDNLLRIAKKIDTPTQTLAQALCSYRVLRQVHDDETGKPIVGPFDRATFGNPQILL
                     TAFCQQYATHKHDVEQEGELALLTQEIRRLASELSTHTTAAWVNYIRGLKAQWEVDQR
                     LFSNLAHQEGQRKVQQHYIDLVEKFTSSSRGLPKTIEEFETVRDLHEKLCQQRGALKL
                     DVPEEVNHFLKAVAGQGATLDMLTPNVLSWLTEEDDPTRYRIRRL"
     gene            30330..34859
                     /gene="dpdJ"
                     /locus_tag="ACHKW0_001722"
     CDS             30330..34859
                     /gene="dpdJ"
                     /locus_tag="ACHKW0_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdJ"
                     /protein_id="HFO7474570.1"
                     /translation="MEQIIRAVLDGLESQEAVLLTWGNTQGFFTETELRNTISQVLQN
                     HPLDANGLTSFGIVKVMIDRGLLLRHQQNGVSVFRTRMAESVRLYSQLRQMFPKHGFN
                     DNWRTAPTLVSDYRFLRRQRKYPKRDRQAAEVLQQLDEIAGFTSTHRQLLSALLSHSS
                     GMYNLAYFQQEACKNVLRGLQQSQHRSTATIVCAGTGTGKTLAFYLPALTFIGSELVA
                     KRKDAVKALAIYPRNELLKDQFIETLRQTRKLNTELAKKGVRKVRIAALFGMVPTNKE
                     AIHRDYMKDAWCRHRNGMACLYIPCPTESCRGKMVWHTADYDKNLERLHCDSCSQTIE
                     PDEVILTRKRMKIELPDILFTSTEMLNKQMGNPFLASLFGIGKNVVPPSMMLLDEVHT
                     YSGVSGAQNGMLIRRWRHLSGATPHFVGLSATLEQATRFMASLTGLEEYLVAEVSPIE
                     DDMETEGAEYMLALRGDPASRSSLLSTTIQASMLTRRMLDNAAVPVSNGMYGTRSFVF
                     TDDIDVINRLYFQLLDAEGRYSNGNINAKKEPLAMLRGDAPNEEKFTFGQQWPLAKMI
                     GHTLDSADRSNVKRTSSQDAGVDHAADLIVATASLEVGFNDPNVGAVIQHKAPRDNAQ
                     FLQRKGRAGRQRTMRPWTVVVLSDYGRDRMAFQCYENLFEPVLKARQLPVGNSYVLRM
                     QAAFATMDWLSSRNEYLNQWNRGIWDDLSVPQDKGKPSEAQSKLADLIEDLLNSQKIQ
                     QEFNSWLAEALGIKDEKLLQSLLWQPPRAIMTAFLPTVLRRLRSNWSRLGIEQADNCR
                     KSTPMPDFIPSALFNDLCLPELQINLLGEVGQEPDAHSMPILQGMKDFAPGRISKRFA
                     IKNIRECHWLVPKKLELKDGSHPFPIDDYCPPDKRESMPDCHITTRTGMQVIPCFRAW
                     EVTASTPPDDLKLSETSNAFLNWHSEIRPPQNGIPAEVPSNNAWQDIFQQVEFYSHQQ
                     HCPIEAVRFATGSRANIKFSDQREDLQIDFKFEHQGEPAAFGFSLWVDAIKFQCRLPN
                     FDLASISNNRELVAGLRTARFLYEVSHDEAISVNTFLGGWLAETVLAGIGSEAVLKNC
                     TPEQAFRAMEHNSARIALIDVPARIFQTLYDAQTGEEIEQDLQQSLRELLSQAPVMAR
                     IADWVELLWQPLDNTWLEWLTLNFTNTLGAALLQTAMQLCPDADDNDLILDLNGGPVR
                     TSLLAPDQREIWLSETTVGGGGIIEKIQQIYREDPRSFFELLDFNLSPGDYETMDNNV
                     WHLLQTMVNPTSSLPACMNEMRLANDHASQVQAQRNLLGELQRSGFMTSHSFLSAINT
                     RLLRPGTDASSDVFLLQLQQDWRQQEQRLGIELPQRVYAFTCSQSSALDQQLQQASQG
                     QHNLESWRLNTCNGLLWPRGHTIRDSWLTWYNPYSFAGPTERLLLAHVVQHSCAQVAL
                     SQPDWMQQCHQHLENNGKCELLAEPGDAINEAIATLLVTPVEMYGLFFYPRVSALRRH
                     NNDTFAMIEIPEAIQ"
     gene            34856..35398
                     /gene="dpdK"
                     /locus_tag="ACHKW0_001723"
     CDS             34856..35398
                     /gene="dpdK"
                     /locus_tag="ACHKW0_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009114670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein
                     DpdK"
                     /protein_id="HFO7474571.1"
                     /translation="MNTRRIFKSRTSRQSEVLDVLSSLLVAEIAQPSAELWIVSPWIT
                     DLDLLDNRTGRFDYLEPAWGQRQVQTSELLARAVANGGTLKVMTNETQHNARFIRQLT
                     ERVANYGMLDNLYIGQKEVLHTKGFLGDHFFLSGSMNLTIGGIVINDEQINLTTDKTA
                     IVQALLAFNDFYQPAQGGVQ"
     gene            35395..37650
                     /gene="dpdD"
                     /locus_tag="ACHKW0_001724"
     CDS             35395..37650
                     /gene="dpdD"
                     /locus_tag="ACHKW0_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpdD"
                     /protein_id="HFO7474572.1"
                     /translation="MNSADSEKFLRAFFHRENNIISWEKIAGGLLEASTQQRLTPWLD
                     DVRNGCFPVILPRVVKGSNDKVTWYAMADEERDYAELRELLDAHVGTSWSDFNRQRTM
                     LDPSDPVEKALIDHYSDRVYKFSSLSLSRIPEIFNGIERLRQQLASRPSYQLTLLRPR
                     GRILRDIRAALRRRDVPQTRLYLQELREAGGIGHINELFIELRCMALEGDAKSLLDST
                     QLRQVLDMPHPLVLAEEVMTVFYQQFLLKYEQLSDLAAQRQAFNNLLVKNVPRLFQVT
                     QGINTPSAIKNILLWLLSDTQSDPARLREQAQQVVAGMKGATDDDRHWIRRLLEAFPT
                     VAVASPPTASIDELLARADEYPESVLKELLLLPVSARGVAIALQCAWVVGSAGAAVSA
                     VSYLQQLAQQERDLLLAKPAFQRYYTALQLMLPSKRTEHDTAGINNWQDWLKEVLNNP
                     QWSGAIEAVEQACGQWNAAAFQPEAFVSLLDKTNNCASMVLRDAIPVLLEWLDNHQTS
                     ASWQSVRLALLQQLALDDNSSREDIFTACELARGYLEGDSNAHDYRELIENLQMMIES
                     SGTTSASAWLDLLELFLVYPTADQLAREQLFNLVLNMFYRNPARYSELQWSLLAQFAG
                     ECGLVCEIPPQILEDSGEKNDEVRVGSTLNDKVVGIYTLTESVAQRVKKQLLADYPQA
                     DIRLNNDKGGSTMLRSLAREADVFIFAWLSSKHSAYYAIKEERGDKPLLQPSGKGSTS
                     MLDCIRSYLKL"
     gene            complement(37707..38069)
                     /gene="queD"
                     /locus_tag="ACHKW0_001725"
     CDS             complement(37707..38069)
                     /gene="queD"
                     /locus_tag="ACHKW0_001725"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HFO7474573.1"
                     /translation="MKTTLYKDFTFEAAHRLPNVPEGHKCGRLHGHSFVVRLEITGEV
                     DKHTGWIIDFAELKAIFKPTLDRLDHYYLNDIPGLENPTSEVLAKWIWNQVKTQLPIL
                     SAVMVKETCSAGCVYRGE"
     gene            complement(38369..38661)
                     /locus_tag="ACHKW0_001726"
                     /pseudo
     CDS             complement(38369..38661)
                     /locus_tag="ACHKW0_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616552.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
     gene            complement(38832..39407)
                     /locus_tag="ACHKW0_001727"
     CDS             complement(38832..39407)
                     /locus_tag="ACHKW0_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7474574.1"
                     /translation="MQNNQRWEPDWFLQSLLNNHIDKMVERYSCLRAFRTDLFYQDHT
                     AKFFLQDHQQLEWDIRKLMNRMMQLEAVVGFFWVIEWTEDHHYHAHVVFWLDGSKTQN
                     TYTWAQQAGLYWSDITDREGWYHRCEFKEHYEANINIPVRYSDPDSITNIRQVLAYMT
                     KIQQKHGLLLYGCNEVPARPLTGRPRSSQSL"
     gene            complement(39606..40199)
                     /locus_tag="ACHKW0_001728"
     CDS             complement(39606..40199)
                     /locus_tag="ACHKW0_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474575.1"
                     /translation="MKDYKPRYEELYRYYQVWLTDFTKLTVRSGMCHQNIYHHHTLTV
                     GSLKFPGEEEVIAIVPQCLHRIIYGRAAAPLTVNEDLSVSLFTQEHLLTHHPMLEGIL
                     LSECVRLKQRPLANKLISLFRQFSGSELRLKLVWLCWYDLMLGNCLDDWTDNLRFKKD
                     KEMDIWINNRQAENPALTIMMDEYVCFAWRTTAEPLS"
     gene            complement(40296..40502)
                     /locus_tag="ACHKW0_001729"
     CDS             complement(40296..40502)
                     /locus_tag="ACHKW0_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7474576.1"
                     /translation="MTTRPSLTEDQFVDMAFITSLLQMTDKWIYKLIKDGVFPKPIKL
                     GRSSRWLKSEVENWLQERIDQSRK"
BASE COUNT        10136 a        10229 c        10431 g        10109 t
ORIGIN      
        1 atcctccgga agatttacgt atagcaatag aaaaatatgg atggaataat tgaactatta
       61 ctgcataaaa taatgtgatg ccgggatgtt tttgtatttc ccggcatctt tatagcgata
      121 tcaattattt actgagcgtc gcgaccatca ccgctttgat ggtatgcatg cggttttccg
      181 cctgatcaaa aacaatgctg gcggcagatt cgaagacctc atcagtcact tccattccgc
      241 catgtaggcc aaactcttcc gccattttct taccaagcgt cgtctggtcg tcatgaaacg
      301 cgggcaggca gtggaggaat ttgacctccg ggttaccggt caactgcatc atcttgctgt
      361 tcacctgata atcacgcagt aatgcaatcc gttctgccca tttctctttt gcttccccca
      421 tcgacaccca tacatcggta tagataaagt cagcaccttc aactcctttg gctacatctt
      481 ccgtcagcgt aatattcccg ccattttgct gtgccagggc gcggcattcc gtaaccagcg
      541 cagcttccgg ccagcatgcc tgtggcgcga ccagacgcaa atccagaccg gtaagcgccg
      601 cagcttcgag catcgaattg cccatgttgt tacgtgcgtc acctgcatag accagcgtca
      661 tttcgttgaa tgctttaccg ggtaaatgct cctgcatggt gaggagatcc gccagcaact
      721 gcgtggggtg aaactcattg gtcaggccgt tccataccgg cacgccagcg tattccgcca
      781 gtgtttcgac aatctcctga ccatagccgc gatactgaat accgtcatac atgcgaccca
      841 gcacgcgggc agtgtcttta atcgactctt tatgaccaat ctgactgccg cttgggccga
      901 gataagtaac gcgagcaccc tggtcatatg cggcaacttc gaaagagcat cgggtacggg
      961 tcgagtcttt ttcgaagatg agcgcgatgt ttttaccagt gagtttggct tcttctttgc
     1021 cgcttttctt atcggctttc agcttcgcgg ctaactgcag caggctgttg agttcagctg
     1081 gcgtgaaatc gagtaatttc aggaaatgct tatgataaaa cccggacata gatccctcct
     1141 gtggctaacg ccttaatgaa ttaaaattca atttatatgg atgattattc atttgcaagt
     1201 ctaaagcata aatctttgtc acaaaggtgg aggcaatgtc agtggtgtgt gacaataaga
     1261 gtatcggcag gacattaaga ggaatgagcc atggcaaacc cggaacaact ggaagaacag
     1321 cgtgaagaaa cacgtttgat tattgaagaa ttactggaag atggcagcga tccggacgcg
     1381 ctgtacacca tcgaacacca tctttccgca gacgatctgg aaaccctgga aaaagcagca
     1441 gttgaagcgt ttaaactcgg ttacgaagtg accgatccag aagagctgga agttgaagat
     1501 ggtgatatcg tgatttgctg cgacatcctc agcgagtgcg cgttgaatgc cgatctgatc
     1561 gatgcccagg ttgaacaact gatgacgctg gcggagaaat ttgacgttga gtacgacggt
     1621 tggggcactt actttgaaga tcccaatggc gaagacggcg acgatgaaga ttttgtcgat
     1681 gaagacgatg acggggttcg ccactaatta attgattaaa atgcccgata gcacacacta
     1741 gcgggcattt ttttagagtt cgcgtagcat ccgcacttca caatctacat ggcccgtgca
     1801 gccaagtgca tagtcgatat gttgaaaacc taaatgttca tacaaaccaa tcgcttcttt
     1861 caaaaaagcg gtcgtttcca gatagcagcg tttgaagccc atctctcgtg cctgctccat
     1921 cgccattaag gccagttttt tcgccagccc tttaccgcga atagccggaa gaaaatacat
     1981 cttctgcagt tcgcaaatgt ccgactgact cccggttaat ggcgcaatcc caccgccgcc
     2041 gaccacttca ccatcgtact caaccaccca atatgcatga cccggctgac tatacacttg
     2101 atacaactcg tccaggttcg gatcggcgac ggtgtagcct ttatcagcgg taagaccgta
     2161 ttcggcggat acctgacgaa tgacgcgggc gatggcggga ttatcctgct gcgtcaggcg
     2221 acgcattaca ggtgattgcg gcgcaatgtt attcatagtt taacccaata aaaataaagt
     2281 aattatacat ttgtttaata ccactctctt cttacgtgcg caagcgactt ataaagtaat
     2341 aaagtttttg ccagcctatt actgataaga gtattgtaac gtggcagaac attttactgt
     2401 ctgtatatat aaattatatc aatggatttt ttaattcagg gaatttttat ggctcaagtt
     2461 attaatgaaa tggatgttcc gtcccattcg tttgtttttc atggtacagg tgagagatat
     2521 tttcttattt gtgtggtgaa tgtgttgtta acgattataa cgctaggtat ctatttgcca
     2581 tgggcattaa tgaaatgtaa gcgttatctc tatgctaata tggaagttaa cggacaacga
     2641 ttttcttatg gaattactgg tgggaatgtt tttgttagtt gtcttgtttt tgttttttgc
     2701 tatttcgcaa tcttaatgac agtgtcagca gatatgccac ttgttggctg tgttttgact
     2761 ttgttactgt tggttttgct tatatttatg gcagcaaaag gactgcgtta tcaggccttg
     2821 atgaccagtc tcaacggcgt aagatttagt tttaattgct ctatgaaagg gttctggtgg
     2881 gtaacctttt tcttgccgat tttaatggcc attgggatgg ggactgtttt ctttatctcg
     2941 acaaagatgc tacatgccaa tagttcaagt agtgttatta tatctgtggt tctgatggca
     3001 atagttggta ttgtttccat tggtattttt aatggtactt tatatagcct ggtaatgagt
     3061 tttctctgga gcaataccag tttcggtata catcgtttca aggtgaaatt agatactacg
     3121 tattgtataa aatatgccat tctcgcattt ttagctttat taccttttct cgctgttgct
     3181 ggttatatta tcttcgatca aatattaaat gcgtatgata gttctgtgta tgcaaatgat
     3241 gatattgaga atttacagca atttatggaa atgcaacgta aaatgataat cgcgcagtta
     3301 atctattatt ttgggattgc tgttagcacc agttatttaa cggtgtcgtt gcgaaatcat
     3361 tttatgagca acctgtcact gaatgatggg cgtattcgtt ttcgctcaac tttaacgtac
     3421 cacggtatgc tttatcgcat gtgtgcgttg gtggtgatat ccgggattac gggcggtctg
     3481 gcttatccac tgctgaaaat atggatgatt gactggcagg caaaaaatac gtatttgctg
     3541 ggcgatttgg atgaccttcc tttaatcaat aaagaagaac aaccagataa aggcttctta
     3601 gccaggattt cacggggaat tatgccttct ttaccatttc tgtaagagat aaaaaaggcc
     3661 ggagcatgct ccggccttcg ttttcattac tgtgttttga ttacagcgcg gcgataacag
     3721 cctgctgttc aatcagcttc gctttcgctt ctgcatagcc ttccagcttt tcacgctctt
     3781 tcgcgatgac cgcttccggt gcgcgggcga caaagccttc attcgccagc ttgttctcga
     3841 tacggctgat ttcaccttca atcttcgcca cttctttcgc cagacgcgcc agctcatctt
     3901 ctttgttgat gaggccagcc atcgggatca gcagctctgc accgtcgatg atcttagtaa
     3961 cggaaaccgg acctttgtca tcggcaggca gcacggtgat actttccaga cgcgccagcg
     4021 tttgcaggaa gccacggttt tcatttacgc gacgttctgc atccgcgctg caaccacgca
     4081 gtagcagctc cagcggtttg cccggcgcga tgttcatttc tgcacggatg ttacgtaccg
     4141 caacgatcgc ctgcttcagc cactcggtgt cggccagtgc ggcttcatca acctgagatg
     4201 catcgtactg cgggaacggc tgcagcatga tggtgtcggc agtgataccg caaagtactt
     4261 tcacacgctg ccagatggtt tcggtgatga acggaatgat cggatgcgcg aggcgcagca
     4321 gaccttccag tacagtcacc agcgtatggc gagtaccgcg cagttctgct tcggtgccac
     4381 cgttcattac cggcttggtc agctcgagat accagtcaca gaactggttc caggtgaact
     4441 catacagaat gcctgcggcg atatcgaagc ggaagctgtc cagcgcttcg cggtacgctt
     4501 tgatggtctg gttgaactcc gccagaatcc agcggtccgc cagcgacagc gtcatttcgc
     4561 cgccgttgaa gccgcaatcc tgaccttctg tgttcatcag cacaaagcgg ctggcgttcc
     4621 acagcttgtt acagaagtta cggtaacctt ccaggcgctt catatcccag ttgatgtcac
     4681 gaccggtaga cgccagtgcc gccagggtga agcgcagcgc gtcagtaccg tgcggttcaa
     4741 taccgttcgg gaactgcttc tcggtgcgct tacggatttt gtccgccagc tgtggctgca
     4801 tcatattgcc ggtgcgtttt tccagcagtt ctggcagcga aataccgtca accatatcca
     4861 gtgggtcaat aacgttaccc ttggatttgg acatcttctg gccttcgtcg tcacgaatca
     4921 ggccggtcat gtaaacggtg tggaacggca cctgcggttt gccattttca tctttgatga
     4981 agtgcatggt catcatgatc atgcgggcaa tccagaagaa gatgatgtcg aagccggata
     5041 ccatcacgct ggttgggtgg aactgacgca gggcgtcggt gttttccggc cagcctaggg
     5101 tagagaaggt ccacagtgca gaagagaacc aggtgtcgag aacgtcttcg tcctgacgca
     5161 gggcaacgtc ggcaccgagg ttattttctt tacgcacttc gtcttcgttg cggccaacat
     5221 aaacgttacc cgcttcgtca taccatgccg ggatacggtg accccaccac aactgacgag
     5281 agatacacca gtcctgaata tcgcgcatcc aggagaagta catgttttcg tactgcttcg
     5341 gtacgaactg aatgtcgccg ttctcaaccg cttcaactgc cggtttcgcc aggacatcgg
     5401 cacgcacgta ccactggtcg gtcagcattg gttcgatgac cacgccgcca cggtcgccgt
     5461 aaggaacggt caggtcatgt ggtttaattt cttccagcag gccgagtgcg tcaaccgcag
     5521 caacgaccgc tttacgtgca gcaaaacgct ccagtttctg gaactctgca gggatttcgc
     5581 tggaataaac gtcagattcg ttacctttgg tatcgaacac ctgggcgctt tcacggatat
     5641 cgccgtcaaa ggtcaggatg ttgatcatcg gcagggcgtg acgtttaccc acttcatagt
     5701 cgttaaagtc gtgcgccgga gtgattttca cgcagccggt gcctttttcc atgtcggcgt
     5761 gttcgtcgcc aacgatcgga atacgacggt taaccagcgg cagaatgaca tatttgccaa
     5821 tcagatcttt gtaacgcgga tcttccgggt taacggccac gccggtatcg cccagcaggg
     5881 tttccggacg agtagtcgca accaccagat aatctttacc atctgcggtt ttcgcgccgt
     5941 cagccagcgg atagcggatg tgccacatcg aacctttcga ttcgcggttt tccacttcca
     6001 ggtcagagat agcggtgcgc agtttcggat cccagtttac caggcgtttg ccacggtaaa
     6061 tcaggtcttc tttatacaga cgaacgaaaa cttctttcac cgcattggac aggccttcgt
     6121 ccatggtgaa gcgttcacgc tcccagtcca cggagttgcc gagacggcgc atctgacggg
     6181 taatggtgcc gccggattcc gctttccatt cccagatttt gtcgatgaaa gcttcgcggc
     6241 cgtagtcgtg acgggtttta ccttcttctg cggcaatctt gcgctcaacg accatctggg
     6301 tagcgatccc tgcgtggtca gtaccgacct gccacagggt gtttttgccc tgcatgcgct
     6361 gatagcggat catggtatcc atgatggttt gctggaaggc gtgacccata tgcaaactgc
     6421 cggtgacgtt cggcggcggg atcatgatgc agaaactttc ctggctttca tcgccattag
     6481 gcttaaagta gccctgcttt tcccagtgct cgtaaagcgg ctgttcgata tcttgtgggt
     6541 tatatgtctt ttccattatt tccaggttgc cgtattcagg ttgaaaccag ccacgcggta
     6601 ggctttatag cgttcgcgcg ccagttgttt cagagaatct tcataaggaa cgaagtctac
     6661 cacttctgtg aaagcggtgg caaaatctgc aaagcttgtt cgcagactaa tcaatatatc
     6721 gcgcgggctg ctgctgcgtt tttgcggcca ggcgatctcc accggcgcac cgccgcgcgg
     6781 tccttctccc gctaaattat gcggaacaaa gctttctgcc ggacgcgccc acagggcttc
     6841 atccagccgg taagcctgct tttcatcttc acaggcgatg agcacgcgct taccactgcg
     6901 ccaacgttct gcggcaattt cacacaccag ctgctcaacg gcgcttaagc catcgacggt
     6961 ggtgtcattg tccagaaggt agaacgtcgc gtttttcata aatggggctt cttgtggtgg
     7021 atttaaatat tgctgcattc ctcaccctaa aagatgagca atgaatttct attgcgctcg
     7081 atcggttccc tctcccttag ggagagggtt agggtgaggg taaaacgcaa acttactctt
     7141 cgccgttaaa cccggcgcgg tttaacagga actgtgccag caacgctacc ggacgaccgg
     7201 ttgcgccttt cgctttacca gaacgccagg cggtaccggc gatatccagg tgcgcccagt
     7261 tgtacttacg ggtaaagcgt gacaggaagc aacctgcggt aatcgcccca ccaggacgac
     7321 cgccaatgtt cgccatatcg gcaaaattgg actccagttg ttcctgatac tcgtcaccca
     7381 gcggtaagcg ccatgcgcgg tcaccggatt gttcagacgc ggcaatcagt tcatgggcca
     7441 gcggattgtg gttcgccatc agaccagtaa tgtgatgacc cagcgcgatc acgcaggcac
     7501 cggtcagcgt cgccacgtca atcaccgctt ccggctcaaa acgctcaacg taagttaaca
     7561 cgtcgcacag taccaggcgg ccttcagcgt cggtgttcag cacttcaacg gtttgaccgg
     7621 acatggtggt taacacatcg cccggacgat aggcacgccc gccaggcatg ttttcgcagc
     7681 cagccagcac gccgataacg ttaatcggca gttgtagctc cgcgaccatc cgcatcacgc
     7741 cgtaaaccgc tgccgcaccg cacatatcgt acttcatctc atccatgcct tctgaaggct
     7801 tgatcgagat accgccggag tcgaaggtta aacctttacc caccagcacg attgggcgtg
     7861 catcttccga cgcgttgcct ttgtactcaa tcaccgacat cagcgattcg ttctgcgaac
     7921 cctgaccgac cgccagatag gaatgcatcc ccagctcttt catctgttgt tcgccgataa
     7981 cgcgggtaat gacattcttg ctgtagctgt cagccagttg gcgcgcctga gaggcgaggt
     8041 aagcggcgtt acagatattc ggcggcatat tgccgagatc ttttgctgct ttaatcccgg
     8101 cggcaatcgc cagaccgtgc tggatcgcgc gctcaccgct ggtcagttca cggcgggtcg
     8161 gcacgttgaa caccatttta cgcagcggac gacgcggttc gctcttgttc gttttcagct
     8221 gatcgaaact gtagagcgtc tcttttgccg tctcgacagc ctgacgcact ttccagtagt
     8281 tgttacggcc tttaacgtgc agttcagtca gaaagcagac cgcttccatt gagccagtat
     8341 cattcagcgt attaatggtt ttctgaataa cctgcttgta ctgacgctca tccagctcac
     8401 gttctttgcc gcaaccaata aggagaattc gctcggaaag tacattcgga acatggtgca
     8461 gcaacaatgt ctgccccggt tttccttcca gttcgccccg acgtagcagg gcgctgatgt
     8521 acccatcgct gattttatcg agctgttctg caatcggaga aaggcgacgt ggttcgaaga
     8581 cgcccacgac gatgcaggca ctccgctgtt tctccgggct accgcttttt acactaaact
     8641 ccatgcacta cgctcctgaa tcttaaagac aacggcggtg gctacagata gaattgcaag
     8701 ctttcgtaac tcatgtccgc tgttgcgatg acttcgtgtt aatcttaacg ttattacggc
     8761 attggcacgt cagaacaaaa tctgagaggt gaatccgttg agtataatta tcttagcgac
     8821 gatttcgacg actcaagaga ataaatgacg tttaagccat gaaacaagct aaaatcctgc
     8881 aaaagacgag tttttacggg cgtatttaaa gtgataatca taagatatct ggtgcgggag
     8941 acgctcaaaa gccagctggc gatactcttc atcttgcttt tgatcttctt ctgtcaaaag
     9001 ttagtgagga tcctcggcgc agcggttgac ggcgatattc cggcgaatct ggtgctctcc
     9061 cttctcgggt tgggcgtgcc ggaaatggcg cagcttatcc tgccattaag cctgttcctc
     9121 gggctgctga tgacgctggg caaactgtat accgaaagtg aaattacggt aatgcatgcc
     9181 tgcggcctga gcaaagcggt tctggtgaaa gcggcaatga tccttgcggt attcacggca
     9241 atcgtcgcgg cggttaacgt gatgtgggcg ggaccgtggt catcgcgtca tcaggatgaa
     9301 gtgttagcag aagcgaaagc gaaccctggc atggcggcgc tggcgcaagg gcaattccag
     9361 caagcgacta atggcagttc ggtgctgttc atcgaaagcg ttgacggcag cgatttcaaa
     9421 gatgtgttcc tcgcgcaaat tcgaccaaaa ggtaatgcac gtccttctgt ggtggtggcc
     9481 gattccggac atttaaccca gctgcgcgac ggctcccagg tcgtcactct caaccaggga
     9541 acgcgcttcg aaggcactgc actgttacgt gatttccgca ttacggactt ccaggattat
     9601 caggcgatca ttggtcacca ggcggtggcg ctcgacccga acgataccga ccagatggac
     9661 atgcgtacat tgtggaacac tgacaccgat cgtgcccgcg cagaactgaa ctggcgtatc
     9721 acgttggtat tcaccgtgtt tatgatggca cttatggtcg taccgctgag cgtggttaac
     9781 ccacgtcagg gacgcgtact gtcgatgctg ccagccatgc tgctgtatct acttttcttc
     9841 ctgatccaga cctccctgaa atcgaacggc ggtaaaggta agctggaccc gacgctgtgg
     9901 atgtggaccg ttaacctgat ttatctggct ttggcgattg ttctcaacct ttgggacacc
     9961 gtgccggtcc gccgcctgcg cgccagtttt tcgcgtaaag gagcggtgtg atgcaacctt
    10021 ttggcgtact tgaccgctat atcggtaaaa ctattttcac caccatcatg atgacgctgt
    10081 tcatgctggt gtcgctgtcg ggcattatca aatttgtcga tcagctgaaa aaagccgggc
    10141 aggggagtta cgacgcgtta ggcgcaggaa tgtatacctt gctgagcgtg ccgaaagatg
    10201 tgcaaatctt cttcccgatg gcggctctgc ttggggcgtt gcttggtctt gggatgctgg
    10261 cgcagcgcag cgaactggtg gtgatgcagg cttctggttt tacccgtatg caggtggcgc
    10321 tgtcggtgat gaaaaccgcc attccgctgg tcttgctgac gatggcgatt ggcgaatggg
    10381 tcgcgccgca gggcgaacag atggcgcgta actaccgtgc gcaggcgatg tacggcggct
    10441 cgttgctctc tacccagcaa ggcttatggg cgaaagatgg caacaacttc gtctacattg
    10501 agcgggttaa aggtgacgaa gagttaggtg gcatcagcat ttatgccttt aacgagaatc
    10561 gtcgtctgca atccgtacgc tatgccgcta ccgcgaagtt tgacccggaa cataaagtct
    10621 ggcgtctgtc gcaggttgat gaatctgatt tgaccaatcc gaaacagatt accggttcgc
    10681 agacggtgag cggcacctgg aaaaccaacc tcacgccaga caaactgggc gtggtggcgc
    10741 tggacccgga tgcactctca atcagcggtt tgcacaacta cgtgaagtat ctgaagtcga
    10801 gtggtcagga tgccggacgt tatcagctca acatgtggag caaaatcttc cagccgctat
    10861 ccgtggcggt gatgatgctg atggcgctgt cgttcatctt tggcccactg cgtagcgtac
    10921 cgatgggcgt gcgtgtggtc accggtatca gcttcggttt tgtcttctac gtactggacc
    10981 agatcttcgg cccgctgacg ttggtttacg gcatcccgcc gattatcggc gcactgttgc
    11041 caagcgccag cttcttctta atcagcctgt ggctgttaat gagaaaatcg taaccaagca
    11101 agggagcgaa agctcccttt cacattgttg ataaagtcaa atacgagttc gatcggtccc
    11161 ctcgcccctt tggggagagg gttagggtga gggggacaga tcgcgcctga cttaccttct
    11221 tctccccccc agcaaactcc ccaacatccc gcgtacaatc tgattcgtca cctggcgagc
    11281 ggcgcttttc gccattgttt gcaccacacc atctttcttc ccgccgcgtg gtccggtagt
    11341 gccaaacaaa atatccttca atccaccaag aataccgtca tccaccgcca cctctttccc
    11401 tttcgtgggg ggattctttt gctgctcggt actggcctga acgccttttt gcagcatctc
    11461 ataggcggac tcgcggtcca cgtcatcctc gtacttgcca tacaccgggg agtgattaat
    11521 caagccatta cgctcatctt ccgtcactgg ccccattcgc gaacaaggcg cgatcaccat
    11581 cgctcgttcc accactgaag gacttccttt tgcatcgaga aacgagatta acgcctcgcc
    11641 ggtccccagt tcctggattg ccttttcggt atcaaatgcc ggattggccc gcatggtttg
    11701 cgccgcagct tttaccgctt tctgatcttt gggcgtaaaa gcccgcaaag cgtgctgaac
    11761 gcgattaccg agctggccga gcacattatc cggaatatca gacgggtttt gcgaaacgaa
    11821 ccagacgccc acgccttttg agcgaataag ccttatcacc tgctcaatct tatccagcag
    11881 tacctgcggg gcatcgttaa acagcagatg tgcttcgtcg aagaaaaaca ccagttttgg
    11941 tttctccaga tcgcccgctt ccggcaattg ttcatacaac tctgaaagca tccacagcag
    12001 gctggcggcg tacagtttcg gcatctgata aagcttctcg gcgctgagga tattgataac
    12061 gcctttaccg ttggtatcgg tgcgcatcca gtctttgata tccagcatcg gctcaccaaa
    12121 gaagtgtgct gcgccttgct gttccagcga caatagcccg cgctggatgg caccaaccga
    12181 tgcgctactg atatttccgt actgattctg gaaggatttg gcgttatcgc cgatgtactg
    12241 ggtaatcgcc cgcaaatctt taaagtcgag cagtaacagc ccctgatcat cagcaatgcg
    12301 gaagatgata ttcagcacgc cagattgcac atcgttgaga ttcaacagcc gcgccagcaa
    12361 cagcggcccc agatccgaaa ccgtcgcccg taccgaatgg cctttctcgc caaagatatc
    12421 ccacaccacc accggattgg catgcggttg ccagtcattg acgccgatat ttttaagcct
    12481 tgtgagcagt ttttccgacg ccgttcctgc ctgcgcgata ccggtcagat cgcctttcac
    12541 atcagccata aacaccggca cgccgatttc cgacaatgac tctgccagtt tttgcagcgt
    12601 aacggtttta cccgtccccg ttgcgccagt aatcagcccg tgacggttag ccattcccgg
    12661 cagtaaaaac agttctgtgt ctggcgtgcg ggcaattaac aggggttcgc tcatgagatt
    12721 tcctccagtt tcctgcctgg agtataggca acggctgcgc actcttcacc gctaaattca
    12781 gcagattgcg agatgccttc caaagagatt ttagtgccgg gcgacaggca cccggcaaag
    12841 ggatcagaag tcggctttca gaaccacgcg gtaacgcgcc ttaccgtcgc gcacatgctt
    12901 gatggcgtcg ttaattttcg acatcgggaa caattcggtg gtcggtgcaa ccttgctgcg
    12961 ggcggcaaag cgcatcagct tacgcagctc ataaggcgtg ccggtagcag aaccagagat
    13021 gctgcggtcg cccgcaatta acgtaaaggc cggaacagac agcggcgtga gaaccgcacc
    13081 gaccgtatgg aaattaccgc cataggtcag cgcctcaaaa taaggctgcc agtcgaggct
    13141 gacgttgacg gtgttgataa tgagatcaaa ctgccccgcc agtgctttga gtgcctgcgg
    13201 atcgcggcta ttcaccactt tatcggcacc catcgccagt acttcctgct ctttcgccgg
    13261 attcgaacta aaggctgtca cctcgcatcc cattgcgtgc agaagtttta tagcgatatg
    13321 ccccagcccg ccaataccaa ttaccccaac gcggctggtg gctgtgatat ggtgcatcaa
    13381 cagtggttta aagaccgtga taccaccaca cagcagcggc ccggcggact cgatatcaat
    13441 attttctggc agtggaatca cccattgcca gtctgcacgc aacttctcgg caaaaccgcc
    13501 gcgattcata atcgtcggca ccgcgccttg ctcgcagttg atctgattac cgctaataca
    13561 ggcgtcgcag tgaccacagc tacgcgccgt ccagccaatt ccgacacgct gaccgacctg
    13621 caaaccttta tcctgcgcgg cgctcccgag cgccaccacg cgcccaatta cctcatgccc
    13681 ggcaaccagc ggatattgtg aaaatcccca ttcgttatcg atcattgaca gatcggaatg
    13741 acagatcccg cagtaatcca cctgcacttc aacatcttgt ggcttcagct caccgggatc
    13801 gtactcataa acttccagtt cgccgcccgc ttcttttgcg gcatagcttt ttatcatcga
    13861 cattttttgg tccttctctg gtgttgtctg ggaagtgtag agcatggcag ggcggggtgc
    13921 ctggagtgtg acaaaggtta cacatcgctg tatgcaatgc tgaaaatttc agcacttagc
    13981 gaggtgcgag caagctggcg cttgcatggt ggcgtgcgac aggtataatc cacaacgttt
    14041 tccgcatacc tcttcagtgc cgaagtggcg aaatcggtag acgcagttga ttcaaaatca
    14101 accgtagaaa tacgtgccgg ttcgagtccg gccttcggca ccatcggaac atcaatagac
    14161 gtcaacggac gtcttttttt gtgcctgaaa tccagtattt acccatcatt cccgctatat
    14221 taactctctc aaggtcaacc gacttcaacg tgcatctacc aacagatgtt ggtacagatg
    14281 atggtatttc cggttcgata atgcttgtac caacagggag gggataagca tggctctaac
    14341 agatatcaaa gtcagaacag ccaagccaac ggataagcaa tataagctga ccgatggcaa
    14401 cggtatgcac cttcttgttc atccaaatgg ctctaagtac tggcgtctgc agtaccgatt
    14461 tggcggaaag caaaagatgc tggctctggg tgtttaccct gacgtatctc ttgcagatgc
    14521 cagagctcgt cgcgatgacg ctcgtaagct tttagcaaac ggcatcgatc cgggagataa
    14581 aaagaaaaat gataagattg agcaggagga agcacgtact tttgagcagc ttgctattga
    14641 gtggcatgcc acaaataaaa agtggtcgga agaacatagt cgacgagtgc ttaaaagcct
    14701 ggaggacaat ctcttccctg ccataggtaa gcggaatatc gctgatctta aaactagaga
    14761 cctgctggcc ccaatcaaag cggtagaact gtcaggacgg cttgaggttg cttcacgatt
    14821 acaacagcga accacggcaa ttatgcgcta tgccgtacag agtggtttac tggactataa
    14881 ccccgcgcag gagatggttg gcgctgttgc ttcaaataac aggcagcatc gacctgctct
    14941 tcagttgaag ctaatcccag aactattacg gcgcatagat agttataccg gtagacctct
    15001 tactagactt gccgtcgaac tcacgctcct ggtattcatt cgttcaagtg aactacgctt
    15061 tgctcgttgg tctgagatcg atttcgaaac atcaatgtgg acaatacctg cagagcgcga
    15121 agctattgaa ggagtaaagc attcacagcg tggttcaaaa atgcgtacgc ctcatctggt
    15181 gccgctttct catcaagctt tagcaattct taaagatgtt tacaaactta gtggtgatcg
    15241 cgattttgtt ttcatcggcg atcatgacca tcgcaaaccg atgagtgaaa acacggtgaa
    15301 caaggcgctg cgcgttatgg gttatgacac gaaggtagag gtttgtgggc acggtttcag
    15361 gacaatggcc tgtagttcat tgatcgagtc tggattgtgg tcgagggatg cagtggaaag
    15421 gcagatgagc catatggaac gtagctcagt acgcgcagct tacattcata aggccgaaca
    15481 tcttgatgaa cggcgactga tgctgcagtg gtgggctgat tttttggatg cgaataggga
    15541 aagcgaagtt agcccttatg actttggcaa gtagtctaat tcctgatgct tgctatgttc
    15601 gaatttgaaa agccggcttg aactataacc catacttttt tataactgta ttattctggc
    15661 gactcagagg tgcactatga aaactttcga tactcaactt gcgatgagca ttgtatcgca
    15721 ctcaacggga ggttttagtt atgacacact cgctagggca ttgatttcta aagataaaaa
    15781 cgaaaattct gacgtccttc ttgagtcatg tcgaaatttt tgtctctctc ttgaggaaaa
    15841 aggtattctg aggcgatgcc aaagatgttc tcattctcaa gatgagtatt tcgagtatat
    15901 tcctcactaa catcagtccc tgaaaatttt ttcagggact gattactccg ttcttgtcat
    15961 cccctttttt ctcctgtagc ttgtgtaaca cgattctggg gggcgattgt gagcaacatc
    16021 aacttaataa ccacatgtac aaacggcaaa cactacggta gtagggatat attgagcctg
    16081 agtcaatttt catcaggccg aaccccttca aatgtgctcg tgaattcttg gtgcaatgct
    16141 ctgaatgaag ctatttctac atctgcaact gtatgtgcag aggatcttta taaaggtggg
    16201 cactgggcga cggctaaagc catcttagat gattatccta ttgatttatg gattctttct
    16261 gctggtttag gacttcttca ccacaaagac aatgttgtac cttacaaagc gacctttgct
    16321 gttggatatg acgaatcaat accattgtat tctcaagaat atgtagggaa aagttttcat
    16381 agaacatggt ggaaagaaat aaccaataga tctattttta aatctaagca tcctacatct
    16441 attgtagaat taatgaaaaa aagaaaaaga gactattata ttatatgtgg ctctccagat
    16501 tatattaatg caattgaact agatatcatt aatggtcttg agtatctagt tgatgcaaaa
    16561 aaacaactgc ttatcataac ttccaaaaaa atcaatggta ggttaactac ataccttttc
    16621 aaaaccaacc aaaatatggc tcagtggctg agatgcaata tgctcatgct taatatcagt
    16681 gtggcaaaat acattgtgaa ggaatttacg tcaaaacaac tcaatgattt aaatgagtta
    16741 tctcaaaagt taaaagaaga actcaaggag ttacctgagc gtgaagtgaa aaaaggaatt
    16801 cgtcgtagtc cggaggaagt taagtctttc attttaaaga ttatggaaaa aaatcctggt
    16861 atcagtgcaa cacacgcgtt gcgtgaattt cgtgactcgg ggaattcatt tgaggaaaaa
    16921 cgttttcggg ctgagtttat ggccctgaga gaggctaaac cttaatgcta cttgcctggg
    16981 tttcatggta gaatcccccg ttttttgagg ctttgcagtg gcgtcacagg agagtaagtt
    17041 gtcgaagcta aagtattttt tccctgatag tcaggatttt atcgatccca gtttcgactt
    17101 ctttcgcgaa acgcgtaacg aacatcgcgt gcgtcaacgt gacgatcagt atccgcatga
    17161 ggtttttcct catccttacg atggtatgct ggtttctaaa gctgtagttg atggtcttgg
    17221 cggcggcgag agcaaatata ctcgtgccca gcgtttgcgt tacttccgta atggaatgaa
    17281 gcactttttc cgtctgccgg aaaatatgga aactatgggt gattgcggag ccttcaccta
    17341 tgttaatcag gacgtcccgc cataccgtgt ggaagaggtg attgagtttt acgaaacttc
    17401 ccgctttaac catggcgttt ctctcgatca tatcgttttt ggctatgaaa aacctggtga
    17461 ggtatttagt ggcgaagtat tgactgagtg tcgccgacgc caggatatca cgctgacctt
    17521 ggctcaagag tttttaaata agtctcaaaa gtcctgtttc accccctttg gcgttgcaca
    17581 tggatggagc aaaacctcct atcgtcagtc tgtagaggcc ctgctggcca tgggatacaa
    17641 aaatatcact atggggggga tggtgccgtt gaaaacggcg cagatcctag agactctcga
    17701 agagattaag ccgttgctga aaagtgatac gcgtgttcac ttgcttggta ttgcccgtcc
    17761 agagagtttc tccgacttta tcaagtttgg tgtgaccagt attgactcga ctacaccgct
    17821 tcagcaggcc ttcaaagatc gaaaaaacaa ctatcatacg cttgatggcc cagcctatac
    17881 cgctgtgcgt gtccctcagt ttgatgcgaa tccaagtttg agtcgcaaaa ttaaatctgg
    17941 cgtgattgat caggatattg cacggcatct ggaaaaggat gccatgaatg ccctgttcga
    18001 atacgataaa ggggctttgt cccttgagaa ggctcttgat gcagtcctgg cttatgaacg
    18061 tctgcattcg ggtgaaaaag aggccgacaa aattcgtgcg gattatgaac gtacgctagg
    18121 agaacgaccc tggaaacaat gccagtgcaa tatttgccag gccatcggta tcaatgtcat
    18181 cattttccgc ggagcagaac gtaaccgtcg ccgcggtttc cataatattc aggtgttgta
    18241 caaccgttta cagcacacat tgtcattacg ctcagaggaa ctgtcatgag tgaatatcgt
    18301 gttcctgccc tgcgaatccg acagggtgag gagaggcaac tctatagcct tgcgatagaa
    18361 ggtaaacaga tcagcaaaat tgcagcgatt tcgcgtattc gtcgcggcga agagagtttg
    18421 gttggttatc agcgtccaga agtacgtaac catattcgcg aaattcagcg ctacattgag
    18481 agcgcaaatc cgatgatacc aaacccggtg attatcgctt ttgataagcg tgtgcgtttt
    18541 gaaccgctta cagataatga cgacatggga catctggtaa tacctttctc cgaagataag
    18601 gattttgaga agccaggttt tattgtggat ggtcaacaac gtactgctgc tttgcgggat
    18661 gcagaaatca attcgtttat gatgccggtc tccgctttta tcgctaatga cgcggaagaa
    18721 cagcgtgaac agttcatgct ggttaactcc acaaaaccgt tgcccaaaac gttgttatac
    18781 gaactggcac cgcataccca cggtcgtttg ccatccgatc tgcaaatgcg taagtttcct
    18841 tcactgctga ctcaacgact gaacttcggc gaaggttcac ttgcagggcg tatcaaaacg
    18901 gcgaccaatc ctgatggtgt tattgctgac aactcgatga tcaaaatgat cgatgccagt
    18961 cttcgcgagg gagcattgta tcgcttccgt gacccggcga cagggttggg ggatgaagag
    19021 aagatggtga aactgctgaa taacttctgg tcggctgtgg aaactgtgtt tactgatgac
    19081 tgggataaaa agcctcgcta ttcccgcttg ttacatggcg ttggtattct tgcacttggc
    19141 agcttgatgg atgaaatcga tcaggtccat caggactata aaggtgaacc tggctggact
    19201 gagatccctt cctacactcg ttttgtcgaa gagctaaacc gaataaagcc gctttgtgca
    19261 tggagcagtg gcgtatggaa cttcggcaca gatattgatg gtcagcctat cgtccgtaaa
    19321 tggaacgaac tgcaaaatct ttcaaaagat atatctttgg ttactgacta tttggtaact
    19381 ggctatatga aaactgtcaa tgcagatatt aaataagggt gacaacccaa ttggcattag
    19441 aagtatcgag tgctgattat cgacgtgggg ggctgcagat atggtgcaga agttcaaatt
    19501 ctgtgcaagc gccagacagc ccccatgctt tcattcttat catcaaacgc tgaccttgtt
    19561 acgatatatg ttcgtaactc atgaaaaatc aagagttttt tgttgaaatt cagtcgtttc
    19621 acgagttttg aaagccggta aataagtcat aggccgcacc ggattaccca ccaatccccc
    19681 ctaaaagtca tagtagacta gtaaccgtga tccccctcct tgacaatcat ctttacgggt
    19741 tgcagtgtat gacattgtcc ttgtattcag tgatattata cgtataaaaa atcaccatta
    19801 atcaaaggct tttaaaatca ctgttacgca cagcaaattt atggatggat atatgcccct
    19861 tgaagttggc gacctcgttg actcgcctta tggaatcggc aaacttattc gcattaacaa
    19921 taatgatgct ctaattaagt atttcgattc tcccgtaaat tatgaaggca tagagcaaac
    19981 tgtcgaagta aaacacctaa aaaaagccga actccacgcg gaaagtctgg tttatcgcta
    20041 cgactacgag gttgcacact ggcagattgc acgtgttgtt ggtgccgtgc ctggaggggt
    20101 acgtttgcat tttcctaaca agcagcagga agacgtcagc ttaaatgata tctttgtgcg
    20161 ctgggatcgc cctatcagcg atgctgcggc ccttctggcc gagcgcatca cctatacccc
    20221 cttctggcaa gatgcccgtc tagcttatca gcatcagatg ctgaaacaac gcaacgcttg
    20281 tgctgggatt acctcttcgc tgtcgtccag tattgaactc gaaccttacc agttggccgt
    20341 cgcgcgtcgg gtgttatctg accctattca acgctatctg ttggctgatg aagtcggcct
    20401 cgggaaaacc atcgaagctg ggctgatcgt gcgtcaacac gtcacggaaa atccgctcgc
    20461 ccactgcgtg gtgattgtgg cgcctggagt tctgcaccgg cagtggcagc aggaactttc
    20521 tgcacgtttt cacctggagg aactgcttaa taagtcgatc catatcattg atatggccgc
    20581 cttcagcaca gcggaccccc tgtcgttccg ccccgatatg ctggtgattg acgaagctca
    20641 tcaggtcggt gcctgggcat gggatgcaga gtacgagcag cgctacgcta gtgtgaacta
    20701 cttggcgcaa caaagtgaaa aattgctatt actatcagcg acacccgtaa ctggcaatga
    20761 aaagaacttc ctggccatgc tgcatctgct ggatgctaaa aattacccac tgactgacac
    20821 tggcttacag aaatttcagc agcgtatcca gcagcgcgaa gaaataggtt cactgttcta
    20881 cgcgtttaac tgtgataacg acaacatgac gctggaagag aacctggaag cgttgactag
    20941 gatgtttcct gaggatgaag tactaaacgg gatggcttca cagttgcttc cccatttaga
    21001 tatgatgcaa ccgctgcgga gtgaggcgcg tgatctgctg atcaataaaa tccgccacta
    21061 cattggtaac cgttaccgtc tgcaccaccg cctgctacgc aaccgccgta gcaaccccgc
    21121 cattagtgtc ctgatgcctg gcttgtccgg cctatcacta aaaaaatatg ccacgacgaa
    21181 cgccagcaac gacatgatga tcgcctggcg cgaatcggca ataatgatgc acccacaaga
    21241 ccccacgttg gcggcaattt atcagttact gtttgaagcc gctatcgcta cgccggaact
    21301 actggcccgg cttgcaaact atcgcttaca gcaggagtca cttggtgccg cgcagggctt
    21361 tctctatagt gattcacagc tcgcgctgtt gcagcatccg ctgtttgatg gcgagaagga
    21421 gatcctgctt gagctgatag accgctgcaa aatggagtgt gacagcaagc gccagcgctt
    21481 aattgatgta ttggaaaaac ttctggatag taacgtcaga gaactccctt atattgtggt
    21541 gttctgtgac cagccatggg tagccgacca ggtatttacc gacctgatga taccatttat
    21601 tggcaaaatc cagcggcata accccgacaa agaagtgcgc tttggcaagg gaagcaaatg
    21661 ccagatctta gtgtgcgatc gccgggctga agaaggtgtc aatctgcacg gcgggcgcaa
    21721 gattgcgctg cattacgact tgttgctgtc gcctaaccgt atggaacagc gtcttggccg
    21781 cttgaatcgc tattgcgcga ctgagagtgc gactactatc ctcagcatgg cgctggtgag
    21841 tgaagcagag ggtctgcaaa atcagtggct gactattctc aatgaagtgt tcgatgtctt
    21901 cgaagagtcg attgcaagcc tacaatactt gattgatgag gaattacagc ggcttatttc
    21961 caatctttac ccgctgggtg aagagagcat gatgcaactc agtgcgcgtc tggctggtga
    22021 ggatggccgc cttacggtgg aaaagcagcg catcgaaatg caggaagtcc tgctgcaaat
    22081 tgatggcgat atcatcgaag ccagagagta tgccgatgca ctggcggaaa tagatgagaa
    22141 tgaagaggaa ttccgccgct gcgctcgtaa tctgataggt aatgtactga aattcgattt
    22201 tattccagat gagaaagatc catcgatatt ccgctatcgc ttcaaccctg gtgatagagg
    22261 gacgggcacc ttagtcagcc cacagcgcct acaaaaacac tgcctgctgg gctttgatct
    22321 caaccaatct agcggaagtt cgcccatgac ttggccactt agctactacc gggctgatac
    22381 ctctcagggt aaaatccgcc cggctcgctt gggtgaacct ttccatgatg ccatcctaca
    22441 aatgctggat tttgaagaac gcggtgttgc tagcgcgatt atccgtatca caccaggcat
    22501 gggcgatgaa aatatttttt tccgctacga ttttctgatt gaagctgatg tccatacacc
    22561 agcactgcaa cgactggcgg accatctgat gcctcctgaa accattacct tgtggctgga
    22621 ccaggccggc cagcaggtca ccaacgcaga ggtgttgatg atacttaatg aagactataa
    22681 accgaaggat aagggcggac gccacctaaa tctcaatgag gaacgctggg cgcagatctc
    22741 ccattgcatc agcgctaaag attggcgcac ctggtgtaac gatagctata gcatagccca
    22801 gcgcctggcg gtggaacagt tcgccacaca acaggctctc agcctgagcc gactggaaca
    22861 ttatctaagc agcgccgcgc tgcatcatgc taaccggcag gagacagggc agatgctgcg
    22921 ccagggtatt gcacagccga aaatgacctg cctggcgacg cgtgccttaa tcattgcatc
    22981 agaagctata ttggatgagg actcataaac cggatgaatt tggcgcaact gattgataat
    23041 gattttattt catcaaggtt gagtgagtgc ctgctgccag aggcgactgc cggacacctc
    23101 gaccgccggg cgttgatccg tgactggctg taccgggaaa aacatgccca ccacaacgat
    23161 aacgagctga ccgtccctga tgatagccaa cctggttggc caagacagga ggagtgggaa
    23221 gccagcggta ttaactgtca acgactaggt cagacctttc tgctcagcgc tggccctgct
    23281 tggtgtccgc agtggctgga gcagggaaac ggcatgcctt ttctcgccag cgatcagcac
    23341 caattctgcc gccaggacac tcgagtaccg atggactatg tcctgcgccg ttacttcaag
    23401 aaggacaact atatcagcta tcaggcaccg ggccagcgcg atgcggtacg ccgggctctg
    23461 ttcgccccac cgggcagtac gctgttggtc aatttgccca caggcaccgg gaaaacactg
    23521 gtagcgcaag cattagctct gacagcctca gctcccgggg cactttgtgt actgatcgcc
    23581 ccgaccacgg cgctggtgtt ggagctggct agccgtttcc ggaagttact gattgaagca
    23641 gggaacaaag cagaatctgt caccggctgg tatagcggct taccggcgca ggataaatat
    23701 gatacgtaca tgcgtatccg tgtgggtgag cagcggctga tcgtcacctc tcctgaagcg
    23761 acctgcagtt ccctgcagtt cgccttattt gaagcagcaa aacagggagg gctgcgccat
    23821 gttattgttg atgaagcgca tattgtcgcc agctggggga acgactttcg cccgcatttc
    23881 cagcaacttg gtggcttaat tcgtgggctg aagcgcgtca gccgtgaagc gaatcttaca
    23941 ccgtgctcaa cactgctgat gagtgcgact atcaccgaag ccagccgtaa aatcctgcgc
    24001 gataccttcg atcaggagat gagcgaactc catgcctgcc atctgcgccc ggaacctgct
    24061 tactggtgtt atcgggcaaa agaccacagc gacaaacggg ataaggtgct gcaatcgctg
    24121 gctcatgcgc cacgcccgct gatcctctac gtgacgcgac cgaaagaagc tgaagagtgg
    24181 gcgcaacagt tgcgtttgca ggggtatcag cgtttggcaa catttaccgg tgatacaccg
    24241 agtgaagtac gccgaagcct gctggaaaaa tgggataaaa accagctgga tatcatgatt
    24301 gcgacctcag catttggcgt aggtatggat aaaaacgacg tgcgcacagt gatccatgcc
    24361 acagtgccgg aaaacatgga ccgcttctac caggaagtgg gacgtggtgg acgcgatggc
    24421 tatgcctgca cctcactgct aatttacaat catcaggatg tcaaacaggc agaacatctg
    24481 tcctcacaaa agttgattgg tcttgagttg ggcctggaac gctggaaaaa gttatggggc
    24541 agtgccgaat cggtgggcga caacctgtat cgtattgata tagagaggat ccatagcggc
    24601 cttaatttcc gcagcaaaca aaataaatta tggaacttgc gtaccgtact gatgatggtg
    24661 cgtgcaggag tgctggaact ggatgccgat ctacaaaacc cgccagacaa aaaagacttt
    24721 gccgatgtta atcgctacga agcggcaaaa gagcaaatca tccgacagaa cggaaattca
    24781 ctggtagtac gcatcctgcg tagtcaccac accggagaag aattgtggaa aacacgtatt
    24841 atcccttccc gtgaagagac gctgcaagct gcaagggcta accataatgc attaatcaac
    24901 tggctgcata cgccactgag cagaccgctg tgtgaggcac ttgtagatct ctactccatg
    24961 gcggaattta ttccgggtca cgcctgtggt ggttgccctg cctgtcgcga taatcaggcg
    25021 cttactagta attatccgag cccaccgacg ctgttgctta acccgccagc gtcggtggca
    25081 cagcaggcgt taagccgctg gcaggcgctc agcggcctat cttactccag ttgctttatc
    25141 acctatccgc cagttaacga cgatgagtgg gaacgtgaga actggtatca ccgcatcgcg
    25201 acgctactgg tgcaactgta tcatagaggc attgtccagc gcgtctgggc agatcctgcc
    25261 ttgttgcata gtatatttag cgccattccg cgcggtgagc gcctgatctt gttgagtgac
    25321 gaattgcctg cacgtgatga gccagcgccg gataacgatg taatggccga gttggcagag
    25381 ttggtacttc ccactcaaga gtcctgcgcc aatggttacc tgccgccttt caacccagca
    25441 ccattacaac tgacgctgat cccgcatgat atgcccgatc ctcaccatcc tggcggggcg
    25501 cattacgttc ttctcgataa tcacaattgg atcaccatcg aacaacttca aaggaaacta
    25561 gacagtgtcg attatcaata atgcccatgg tggcagccag tatgccgtgc tggccgcatt
    25621 ttatcaggtg ctgaaagcca gcccgaagaa ccaactaccg cgtgaaaagc tgatggcgct
    25681 ctgtgcaccg gaaacattgc aaaccggcga ctcacaaaat gcacgtggta aagcgtctca
    25741 ggaattaaga gcatggctgg acttggggtt actgacggaa gaaaaagtag aagagacaac
    25801 acaaatcagt ttcaacgcga actatttcag cacaaaaagt atgccgctgc gccaggctgc
    25861 gcgccgctgt ttacaggcac aggaaaataa cagtgacctg agctcacgcg atgaacgcgc
    25921 cgtcgacctt acccttttgg ctgcattgct gctggcgctg gatgtctatc agaacgcaga
    25981 aatcaaatcg ggcaaccttg aagtgctggt gaacacgttt atacctgatt ttcgtatcaa
    26041 cagtaatgaa attgctgtcg ttccggggta tctcagttgg ctgggctttg ctcaacaact
    26101 cactcgcgac agttacgcca ttgaccccac cgattctatt cgtgaagagt taccgttcct
    26161 tatgcgtgtt ggcgaacaac tttccatcgg cgaagtgctg caacgtctta atcgggcgct
    26221 cccggtgcta gatggtggca gttatcgcca gcaggtcgag gagcgtatca gtctgtatgg
    26281 ctggcactct cttagcccta accgcctttc cacctcgctg tcgcgtgcgc tgctgcggct
    26341 tcagctttcc ggagtaatca acatgaaggc tgagtctgac gccagcggcg caatgcagtt
    26401 aaccggccgg aacggcagcg tgctgcgtac cgtaacccat ctgaccttga atgatgtggg
    26461 agtccactaa tgccacaact acagagctac tggcccagcg agcagaacgt tactgcctgc
    26521 atcctaaccg aggctgagtc actggctgac agccagttgc tggcggtgca tgaaccgatg
    26581 cgtctcgacc gcattgagtt tcaaagtggt aaggtcacgc aggtgggaga gggggcgctg
    26641 ctggactttc tgcttgaaca taaccgcccg ctgccgctaa ttggcgcttc tggcgtgggg
    26701 aaatcccact tggtacgctg ggttcatgct cagctcaaac gtcgtgaaga tcatgcgaaa
    26761 taccacatta tccgcatccc caaaaatgct tctctgccac gtgtacttgc cgccattctg
    26821 gaaggtcttg acggcgagga gtatcagagt attcgtgaaa aggttaacgg agttggaaag
    26881 caattaatcc ccgaaaacgt tgccgaacat attgctctta aattgcgtca agcgctaaac
    26941 gcagcatata cagatgccat tgaagagctt cgccaagaaa aaatgggaaa aattcagctg
    27001 gaagaaagca gaaaaaagca gctggaagaa attcaacaac acgcagcatc atcccgactg
    27061 cccgcgctgt tttttgacag cgtgatgacg cagtatttca ctgctcctgg cgcctgtctt
    27121 cacaatatag cgcagcgctt ctgccaggga gctaacgacg acagtattgc caacttgcgg
    27181 tatgaaatgt ctgcggaaga cttcaccttc aaaggactgg acttcaggca ggtttcacca
    27241 acagtgcttc cctacctgcg caaccaacag ttgcttacca gtgatgaaaa aaagcaagca
    27301 gcggctcgcg ttgtcaacgt ggtgcgcagc caggcgttag aagacacctt cgtcgaacta
    27361 tttagcttta accgggccaa tttccaggat ctgatgcgta tgatccgccg acagttgctg
    27421 gcagaaggtc gcgacctgat ccttcttgtg gaagatctta ccgccacctc ggcgattgaa
    27481 gatgtcctga tcgcctgtct gatagaggaa gatgagtacg acggcaaaaa ggttttatgt
    27541 actctaagat cgattattgc ggttactgag ggtcacgata gctttaagcg cctacgtaat
    27601 acactaggca cccgcgcccg ctacgaatgg gtgatccagc agcatgccag cgaaaacgat
    27661 gtcgcgctga aaaaacgcgt tattgacttt tgcggccgct atctcaacgc tgcccgtcac
    27721 ggcgctgctg ctctggaaac ttatcacctc cagcaagacg cgcagtgtta tcaggagatt
    27781 cctgtatggc aggatcagga ggtgctggag aacgaatctg ctgcgccagc gctggccagt
    27841 tttggcagca gtagcattgg tcatccgctg tttcccttca acccagcagc cattgggcag
    27901 ttggtagaac gacactgcaa agttaaagaa caaggcctag tgtttatccc gcgtaatatt
    27961 ttgcatgaaa ttttgcgcga gccactgaaa aagtatcgcc agaattatct ggacgggcag
    28021 ttcccgccat cacaatttgc tgagattgta tgtaacccgg cattacagca gagggtacgc
    28081 ctggaaggcc tcagccagcc tgagcggatt aattcactgc tggctgtttg gggggggaat
    28141 agtacaaacc tggcaggtct tacgcccgcc atctgtaaag agtttggtct gccggatgcg
    28201 gcagtactgt tggggggcgg tggaacgcca ccggaaccag caccggaacc agtaccggaa
    28261 cctgcaccag taccggaatc agtaccggca ccaccatcac cacttgatga atggaaactc
    28321 gtgctggata aatggcacca gagcaagacg ctcgagcaaa ccagagcccg cgacattcgt
    28381 aagtggctcc tggaagcgct gaatgcccgt atcgactggt caactgagct tatccagacc
    28441 tctctcatgg ccagctcagc aaaaactcag ggtaggatcc acttgcccgg cgtgcttgta
    28501 gggaatcagt cacatccgct ggtgaagctt gaagatactc ctgagttgcg tagcgcctgt
    28561 ctggcgatgg cgcacttcaa ccattatcag agttgggatt atgacggcag tgagatcgac
    28621 tacgccgcct ttcatagttt catggattgc attgagcacg aagtaaaact caaggtgttg
    28681 caggaagaac gcgaggagct ttctagtatc atcaacgatc tgcagttaag tggtgcattc
    28741 cttgggttgg atggattatc agcatcccac ccggccacgc tggtgcaggc actgttaacg
    28801 gaaccgcaag tcgcaaacag caaccatcca ctgctggatg agttcttaac ccaagcgcgc
    28861 gacaccgtga ttgatgagca tctgaaacgt cgcgagcgtt taatcgcgct tacggcggcg
    28921 aaaaaaccgg gtgctcagcc gtatgctatt aacggcttaa tgctggtgga ggcgtctaag
    28981 aaacaatcgc cagacgctgg caaacaaccg ggcaagatcc atgaagtcaa aagtaagctc
    29041 ggtggctacc gcaccctgct attgaagacc caagagagca tcactgaacg cctgagcgac
    29101 ggcaacagcg ccagcctggc agcggtgaac ctctgcgaca ccatgaagga gatagtcgaa
    29161 ctggcgaata gcgcggacgt cgcccgtcca atgggtgaaa ctgtaccggt actgctgaag
    29221 ttgctgaaga gctggaaaga tccagagctg aaaaaactga tcagacaact ggaaaaactg
    29281 acgttgccag ccgataactc gcacatgttg atgagagaaa tccttgccat caatggcgag
    29341 cagtttgagg cgtttcgaca gttattagag aattggggca agttcgaaga tgccaccgac
    29401 aagtatgtgc aacagcgtat aagcgataag ggcggagacc aagttgagga tgcagaaaac
    29461 caagttacag ataccctggc aaacctggct gaacagttaa cactgctgga ggcggactta
    29521 tcatgagtga agtaaacatc aatgacgtgg taactcccgt agccagcgta ccggaagaac
    29581 gcggtatttt tgcacggcgc agcggtgatt ttaaagcacg ccttcaacgc taccaacaaa
    29641 tggagaaagc tcaggatatc cgcgataacc tgctgcggat agcaaaaaaa atcgatactc
    29701 cgacccaaac actagcacag gccctctgta gctatagggt gttaaggcag gtccacgacg
    29761 atgaaacggg taagccgatt gttggtccgt ttgaccgtgc gactttcggc aatccacaaa
    29821 tcctgctgac ggccttttgc cagcagtacg cgacgcataa acatgatgtt gagcaagagg
    29881 gtgaacttgc cctgctaact caagaaatta ggcgcttagc cagcgaactc agcacgcaca
    29941 ccaccgcggc gtgggttaac tatattcgcg gattaaaagc tcagtgggaa gtggatcaga
    30001 gactgttcag taacttggcg catcaggaag gacaacgaaa agtacaacag cactacattg
    30061 atctcgtgga aaagttcacc agcagtagcc gggggctgcc gaaaaccatt gaagaatttg
    30121 agacggtgag agacctgcat gagaaactct gccagcaacg cggagcactt aagctggatg
    30181 tgccagaaga ggtcaatcac ttcctcaaag ccgtagccgg gcaaggtgcc acgctcgata
    30241 tgttgacacc taatgtactc agttggctga ctgaagaaga cgacccgacg cgctatcgta
    30301 taagacgcct gtaaggagcc gtagtttcaa tggaacagat tattcgcgca gtacttgatg
    30361 ggctggagtc gcaggaggcg gtactgctca cttggggtaa cacacaaggt tttttcactg
    30421 agaccgagct ccgtaatact atttctcagg tcctacagaa ccacccgctc gacgccaacg
    30481 gcctaactag ttttggcata gtgaaagtta tgatcgatcg cggattgctg ctgcgccacc
    30541 agcaaaatgg tgtttcggtg tttcgtaccc gcatggcgga gtccgtgcgg ctttacagcc
    30601 agttgcgcca gatgtttcct aagcacggct ttaatgataa ctggcgtact gcaccgacgt
    30661 tggtgtccga ctatcgcttc ttacgccgcc agcgcaaata cccgaagcgt gaccggcagg
    30721 ctgctgaagt tttgcagcaa cttgatgaaa tagcaggttt tacctctacg caccgccaac
    30781 tactatcagc cctgctcagc cactcgagcg gaatgtacaa cctcgcctac tttcaacaag
    30841 aagcctgcaa aaacgtactg cgcggattgc agcagagcca gcaccgctcg actgctacca
    30901 ttgtctgcgc cggaactggg actggtaaaa ctttggcgtt ctatctgccc gctctgacct
    30961 ttatcggcag tgaattggtc gccaaaagaa aagacgcggt gaaggctctg gctatctacc
    31021 cgcgcaacga actgctgaaa gatcagttta ttgaaacgtt gcggcagacg cgcaaattga
    31081 acactgaact ggcaaagaaa ggtgtgcgca aagtgcgtat cgccgcattg tttggtatgg
    31141 tgccaactaa taaagaggcg atccatcgcg actacatgaa agatgcttgg tgcaggcatc
    31201 gcaatggcat ggcttgtctg tacattccct gcccgacaga gagttgtcgc ggtaaaatgg
    31261 tctggcacac cgccgattat gataaaaacc ttgaacgcct gcattgcgac agttgtagcc
    31321 agaccatcga gcctgacgaa gtgatcctga cgcgtaagcg tatgaaaata gaactgcctg
    31381 acattctatt cactagcacc gaaatgctga ataaacagat gggcaacccg tttctggcat
    31441 cgctgtttgg catcggcaaa aacgtggtcc caccgtcgat gatgctgctt gatgaggtgc
    31501 atacctattc cggcgtcagc ggtgcacaga acggcatgct gatccgccgc tggcgacatc
    31561 tgtccggtgc cacaccgcac tttgttggac tgtccgcaac gttggagcag gcgacgcgct
    31621 ttatggcttc gttaaccggc ttggaagagt acctggtggc ggaagttagt ccgatcgaag
    31681 acgatatgga gacagagggc gcggagtata tgctggctct acgtggcgac ccggcgtccc
    31741 gctctagcct tttgtccacc accattcagg cctccatgct aaccaggcgt atgctcgaca
    31801 acgccgcagt gccggtcagc aacggtatgt acggcacgcg cagttttgta tttacagatg
    31861 atattgacgt cattaaccgc ctctatttcc agctactgga tgccgagggt cgttactcga
    31921 atggcaatat caatgccaaa aaagaaccgc tggcgatgct gcgcggcgat gcgccaaatg
    31981 aagagaaatt tacctttggt cagcaatggc cgttggctaa aatgattggc catacactcg
    32041 attctgctga ccgcagcaac gttaagcgca cctcttcgca ggatgctggc gtcgaccatg
    32101 ctgcggatct gattgtcgct accgcgtcac ttgaggtcgg atttaacgac ccgaacgtcg
    32161 gtgccgttat ccagcataaa gccccgcgcg ataatgccca attcctgcag cgtaaagggc
    32221 gtgctggacg tcaacgaacc atgcgcccgt ggaccgtggt tgtgctgtcc gactatggcc
    32281 gtgatcgcat ggcatttcag tgctacgaaa acctgtttga gccggtacta aaggcgcgcc
    32341 aactgccggt cggcaacagt tacgtactgc gtatgcaggc ggcctttgcc actatggact
    32401 ggctctccag ccgtaacgag tatcttaatc agtggaatcg tggtatctgg gatgacctat
    32461 ccgtcccgca ggacaaaggt aagccatctg aagctcagtc aaagctggcc gatctgattg
    32521 aagaccttct gaacagccag aaaatccagc aggagtttaa ctcatggttg gctgaagcac
    32581 tggggattaa ggacgaaaaa ctgctgcagt cattgctgtg gcaaccccca cgcgcgatta
    32641 tgaccgcgtt tcttcctacg gtacttagaa gactgcgttc caactggtcg cgccttggca
    32701 ttgagcaggc cgataactgc cgcaaaagca cgccgatgcc tgattttata ccgtccgcgt
    32761 tgtttaacga cctctgcctg ccggagttac agattaacct gctaggtgaa gtcggtcagg
    32821 aacccgatgc gcacagcatg ccgattttgc aggggatgaa agattttgcc ccggggcgta
    32881 tcagcaagcg ctttgccatc aaaaacattc gcgaatgcca ctggctagtg ccgaaaaaac
    32941 tcgagctgaa agatggttcg catccattcc cgattgatga ttactgcccg ccagacaaac
    33001 gggagtccat gcccgattgt cacattacca ctcgcactgg catgcaggtt atcccttgct
    33061 tccgcgcctg ggaagtgact gccagcacgc caccagatga ccttaaactg agtgaaacct
    33121 caaacgcgtt tcttaactgg cacagcgaga tccgtccacc tcagaacggc atccctgcgg
    33181 aagtgccatc caacaatgcc tggcaggata ttttccagca ggtggagttt tacagccacc
    33241 agcagcactg tccgatcgaa gccgtgcgct ttgccaccgg atcacgtgcc aatattaaat
    33301 tcagcgacca gcgtgaagac ctgcagattg acttcaaatt tgaacaccaa ggcgaacccg
    33361 ctgcgtttgg cttctcgctg tgggtagatg cgataaaatt ccagtgtcgg ttacctaatt
    33421 ttgatctcgc tagcatcagc aacaaccgtg aactggtggc cggattgcgc acagcgcgct
    33481 tcctgtatga agtcagccac gacgaggcaa tcagtgttaa tactttcctc ggcggttggc
    33541 tggcagaaac tgtcctggca ggtattggca gcgaagcagt attgaaaaac tgcaccccgg
    33601 agcaggcgtt ccgcgcaatg gaacacaaca gcgcaaggat cgccttgatt gacgttcctg
    33661 ctcgaatctt ccagacgttg tatgatgcgc agaccggtga agagatagag caggatctac
    33721 agcaaagctt gcgtgaactg ttgtcccagg ctccggtgat ggcaagaatt gccgactggg
    33781 tggaactgct gtggcaaccg ctggataaca cctggttgga gtggttgacc ctcaacttca
    33841 ccaacacact gggtgcggcg ctgctgcaaa ccgctatgca gttgtgccca gatgccgacg
    33901 acaacgacct aatccttgac ctcaacggtg ggccggtgcg cacttcgctg ctcgcgcctg
    33961 accagcgcga aatctggctg tcggaaacca ccgtgggcgg tggcggtatc attgagaaga
    34021 tccaacagat ttaccgtgaa gatccgcgca gtttcttcga gttgctagac tttaacctca
    34081 gtccaggcga ctacgaaaca atggacaata atgtctggca cctgctgcaa accatggtaa
    34141 acccgacatc atccctgcct gcgtgtatga acgaaatgcg ccttgccaac gatcatgcca
    34201 gccaggttca ggcgcagcgc aacttgctgg gagaattgca gcgcagcggc tttatgacca
    34261 gccacagctt cctgtcggcg atcaacaccc gcctattacg gccggggacc gatgccagca
    34321 gcgatgtctt tttactgcaa ctgcaacagg attggcgaca gcaggagcag cgactgggta
    34381 ttgaactacc acagcgtgtc tatgccttta cctgtagtca gtccagcgcg cttgaccagc
    34441 aactacaaca agctagccaa gggcagcaca acttggagag ttggcgttta aatacctgta
    34501 atggcctact gtggccacgt ggccatacca ttcgtgatag ctggctaact tggtacaacc
    34561 cttacagctt tgccgggcca accgagcgcc tcctactggc ccatgtggtg cagcacagtt
    34621 gtgctcaggt ggcgctcagc caaccagact ggatgcagca gtgtcaccag catctggaaa
    34681 ataatggtaa atgcgaatta ctagccgaac cgggcgatgc aataaatgag gccattgcta
    34741 ctcttttagt caccccagta gaaatgtatg ggttgttctt ctacccgcgc gtcagtgccc
    34801 tgcgccgcca caacaatgac accttcgcca tgattgaaat tccggaggcg atccaatgaa
    34861 tacccgtcgt atttttaaat ccagaacctc acgccagagt gaagttcttg atgtactcag
    34921 ctccttgttg gtagcggaga tcgctcagcc cagcgcggaa ttgtggatag tctcgccgtg
    34981 gatcaccgac ctcgacctgc tggataatcg caccggccgt ttcgactacc tcgaaccagc
    35041 ctggggacag cgtcaggtgc agacctcaga actgctggcg cgtgcggtag ccaacggcgg
    35101 tacgcttaaa gtgatgacca acgagaccca gcataatgca cgctttatcc gccagttaac
    35161 cgaacgcgtc gccaactacg gcatgctgga taacctgtac attggccaga aagaggtact
    35221 gcataccaaa gggttcctcg gagatcattt cttcctaagc ggttcaatga atctgacgat
    35281 cggcgggatc gtgatcaatg atgaacagat taacttgacc acagacaaaa ccgccatcgt
    35341 gcaggcgttg ttagcattta acgactttta ccaaccggca cagggaggtg tgcaatgaac
    35401 agcgctgata gcgaaaagtt cctgcgagcc tttttccata gagaaaacaa tattatctct
    35461 tgggaaaaaa tcgcgggtgg cctgctagaa gcatcaaccc aacagcggtt gaccccctgg
    35521 cttgacgatg tccgcaacgg atgtttcccg gtgatcctgc cgcgcgtagt aaagggcagc
    35581 aatgacaaag tcacctggta tgcaatggcc gatgaggaac gagactatgc ggagttgcgc
    35641 gaattattag acgcgcacgt cggtacctcg tggtcagact ttaaccgcca gcgcacgatg
    35701 ctggatccga gcgacccggt ggaaaaagcg ttgatagacc actatagcga tagagtatat
    35761 aaatttagct ctctttctct gtcgaggatc ccggagattt tcaatggcat agagcgctta
    35821 cgccaacaac tcgcaagccg cccgagttac cagctcacac ttctgcgccc gcgtggccgt
    35881 attctgcgtg atattcgtgc cgccttgcgc cgtcgtgatg tgccgcagac tcgcctctat
    35941 ctgcaggaac tacgtgaagc tggtggcata ggccatatta acgaactgtt tatcgaactg
    36001 cgctgtatgg ctctggaagg tgacgccaaa agcctgctgg attcgactca gttgcgacag
    36061 gtcttggata tgccgcaccc gttggtgttg gctgaagaag taatgacagt cttctatcag
    36121 cagtttctgc tgaagtatga gcagttgagc gacctcgccg cccaacgaca agcatttaat
    36181 aatttgctgg tgaagaacgt accgcgtctg ttccaggtga cgcagggtat aaacacgcca
    36241 tcggcaatca aaaatattct actgtggtta ctttctgaca cacaaagtga tccggcaaga
    36301 ctgagggaac aggcacaaca agtggttgcc ggtatgaagg gggcaacaga cgatgaccgg
    36361 cactggatcc gccggttgtt ggaagcattc ccgaccgttg cagtggcctc cccaccgacg
    36421 gcgagcatcg atgaattgct tgccagagcc gatgaatatc ctgaaagcgt tctgaaagag
    36481 ttactattgc taccggtcag cgctcgcggc gtagccattg ctttgcaatg tgcctgggtc
    36541 gtaggcagtg caggagctgc tgtgagtgct gtcagttatc tgcaacaact ggcacaacaa
    36601 gagcgcgact tgttactggc gaaaccggcc ttccagcgtt attacactgc cttacagttg
    36661 atgctgccat ccaaacgaac agaacatgat actgcaggta tcaataattg gcaggactgg
    36721 ttgaaggaag tgttaaataa ccctcaatgg tcgggggcga tagaagcggt agaacaggcc
    36781 tgcggccaat ggaacgcagc ggctttccag cccgaggcat ttgtttcgtt gctggataaa
    36841 accaacaact gcgccagtat ggtgttgcgc gatgccattc cagtactgct cgagtggctg
    36901 gataaccatc aaacaagtgc ttcctggcag tctgtccggt tagcgttgct gcaacaactg
    36961 gcgctggacg acaatagttc tcgcgaagat atctttactg cctgtgaact agcacgcggt
    37021 tatttagagg gcgatagtaa tgcgcacgat taccgcgaac taatagaaaa cctgcagatg
    37081 atgatcgaga gttcaggtac caccagcgct agcgcctggc tggatttgct ggaactgttc
    37141 ttggtctacc caactgctga ccaacttgcc agagaacaat tattcaatct ggtactcaac
    37201 atgttttacc gcaacccagc ccgctatagt gaattgcagt ggagcctatt ggctcagttt
    37261 gctggcgagt gcggacttgt ctgtgaaatt cccccacaaa ttttagagga cagtggggaa
    37321 aaaaatgatg aggttcgtgt cggatcaaca ttgaatgaca aggtagtggg catttataca
    37381 ttgactgagt cggtagcaca gcgtgtgaaa aaacaactac tagccgatta tcctcaggcc
    37441 gatattcgcc tcaacaacga taaaggcggc tcaaccatgc tgcgttcatt ggctagagaa
    37501 gcggatgtat ttatttttgc ctggcttagt tctaaacatt ctgcttatta cgcgataaag
    37561 gaagagcgag gagataaacc tttattgcaa ccttccggaa aaggcagtac cagtatgctg
    37621 gactgcatac gtagttatct taaattgtag taaaggtaaa aaacttagct gtaacctata
    37681 acgtcacagc taagttcaat ctcgggctat tcaccccgat aaacacaacc cgcactacag
    37741 gtttctttca ccatgacagc actcagaatt ggcaactgtg ttttaacctg attccatatc
    37801 catttggcca gtacttcgct ggttgggttt tccaagccag ggatgtcgtt gagatagtag
    37861 tggtctaggc gatctagcgt cggcttgaaa atggccttga gttcagcgaa atcaataatc
    37921 caacctgtgt gtttatcaac ttcaccagtt atctcaaggc gcacgacaaa agagtgtccg
    37981 tgtaagcggc cgcatttatg gccttcagga acattgggaa gacggtgtgc ggcttcaaat
    38041 gtgaaatctt tgtataacgt agttttcatg gtgacctcat tttttgttat acagtaacaa
    38101 taagaagcaa gatttcctag catttaagaa tgagcattaa cacgcttgcg tattccgacc
    38161 caaaccggat tgttttcgat tgtgagcctc tttgcaggat gagcatgcaa ctcaattgga
    38221 cgttatcgct agccacagcc ctacatcgac tagtttcacc gcttcctacg taagtttaaa
    38281 gattggcggc aaaaaagagc ttcagcattc tcatgacgct atttcagttg gataactgaa
    38341 atctccgcta cgacaactca ggcaataact aactggtctt gcattgtgcg cagagccagt
    38401 cggctttact gaccctgagg atagcattgg ggatgaagtg ctgtagccgt ctggctaatc
    38461 aggatttaca tacgggcagt gctgtcgccc gttctccgtt agatcatcgg gccggtgttc
    38521 aaaccacggg cgttcggtaa tgtattcagg atggtattgc agcacgctac tgctgtggca
    38581 ggtataacga tcgtcagggg tggtcttcgc agtacgggca ccggcgctcc cgtcgaaagc
    38641 gatataggat ttggcgtaca tagtattgtt ccttaccgcc agacgacacg caggggcaga
    38701 cgtcccggtg ctagcagcgt ggttggatgt gaatggcagg tcaatttagt aatatcagaa
    38761 gagggctaag cctgtgctgg atgtcattac tgtctgttag ttttcacact atgaggtact
    38821 gacgtaaaaa gtcagagtga ctggctgcta cggggtctcc ctgttaatgg gcgtgcaggg
    38881 acctcgttgc agccgtagag cagcaggcca tgcttttgct gaattttggt catatacgcc
    38941 agtacctgtc ggatgttggt gatactgtcc ggatcgctgt aacgtaccgg aatgttgata
    39001 ttggcctcgt aatgctcttt aaactcacat cggtgatacc agccttcccg gtcagtgata
    39061 tcgctccagt acaaaccggc ttgctgtgcc caggtatagg tattctgagt tttactacca
    39121 tccagccaga acacgacgtg ggcatgatag tgatggtctt ctgtccattc gataacccag
    39181 aagaaaccga ccaccgcttc gagttgcatc atacggttca tcagctttcg gatatcccat
    39241 tcgagctgct ggtgatcctg caggaaaaac ttagcggtat gatcctggta aaacagatct
    39301 gttcggaatg cccgcaggca ggaatagcgc tctaccattt tgtcgatatg gttgttcagg
    39361 agtgactgta gaaaccagtc tggctcccat cgttgattgt tttgcatagg atgagtttct
    39421 ttagtgctgg caagggccag ccagtggaat gaaccaggcc ttaaggaagg accatggcag
    39481 agggagttgt atggattagg ggggatagaa gtaaaaaggg agtgcctatt aattatatta
    39541 attaacctaa attaattttc actccccaat aaccctcaaa tcccttgcag gactatggat
    39601 gaagcctaac tcagtggttc agctgtcgtc cgccatgcaa agcagacata ctcgtccatc
    39661 ataatggtca gcgcagggtt ttcagcctgc cggttattga tccagatatc catctctttg
    39721 tcttttttga acctgaggtt gtcggtccag tcatcaaggc aattccccag catcaaatcg
    39781 taccagcaca gccagaccag cttcagacgc agctcactac cgctgaactg gcggaacaaa
    39841 ctgatgagct tatttgccag agggcgttgt tttaaacgca cgcactcaga cagcaaaatc
    39901 ccctccagca tgggatggtg agtcagcaga tgctcctggg taaataaact gacgctcagg
    39961 tcttcattaa cagtcagtgg ggctgctgct ctaccataaa taatccggtg caggcactgg
    40021 gggacaatag caataacctc ttcctctccc ggaaacttaa ggctgccaac cgtcagggta
    40081 tgatggtggt agatattctg gtgacacatc cccgatctga ccgtcagctt cgtaaaatca
    40141 gtcaaccaga cctgataata tctataaagt tcttcgtaac gaggcttgta atctttcata
    40201 gttcatattc cttaaggctt aaatgacaga cagcagaaaa cctccggagt aaccggatag
    40261 ttattattct gctgagggtt tagtctggtg ggaggttatt tacgagactg gtcgattctt
    40321 tcctgtagcc agttttcgac ttcgcttttt aaccatcggg agcttcggcc cagcttgatg
    40381 ggtttgggaa agactccatc cttaatgagc ttataaatcc acttatctgt catttgcagc
    40441 agactggtga taaatgccat gtcaacgaac tggtcttcgg tcagtgaggg gcgggtagtc
    40501 atgatttgta tctccggtta agaaaggtaa gccggattag tgaagtttga taacgacgac
    40561 tgctccggga catatccagg tggagatacg taaaaaaaga gagttatgat tacctctgtt
    40621 cctactttga tatccgtgca ggtctttttc aaataaagaa gataatgaag tgtgatttct
    40681 ggtagggcca ttttttagat gggaaattgt atggcaacta gttgctgtct atattggaag
    40741 cggttgccac tattaatcaa aaaatgtaag aagagtgtgt attaactaga atggtaataa
    40801 tttctatata gcgctgaaga gagaaaagtg atcatcattc tcatttacct atgtaaacta
    40861 catcacataa ttgtcatttt tcccttggtt cttatacatg aatgt
//
LOCUS       DAZFXT010000031        40203 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.031, whole genome shotgun sequence.
ACCESSION   DAZFXT010000031 DAZFXT010000000
VERSION     DAZFXT010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40203
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.031"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            319..405
                     /gene="leuL"
                     /locus_tag="ACHKW0_001730"
     CDS             319..405
                     /gene="leuL"
                     /locus_tag="ACHKW0_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HFO7474577.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            498..2069
                     /gene="leuA"
                     /locus_tag="ACHKW0_001731"
     CDS             498..2069
                     /gene="leuA"
                     /locus_tag="ACHKW0_001731"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HFO7474578.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2069..3160
                     /gene="leuB"
                     /locus_tag="ACHKW0_001732"
     CDS             2069..3160
                     /gene="leuB"
                     /locus_tag="ACHKW0_001732"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HFO7474579.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3163..4563
                     /gene="leuC"
                     /locus_tag="ACHKW0_001733"
     CDS             3163..4563
                     /gene="leuC"
                     /locus_tag="ACHKW0_001733"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HFO7474580.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4574..5179
                     /gene="leuD"
                     /locus_tag="ACHKW0_001734"
     CDS             4574..5179
                     /gene="leuD"
                     /locus_tag="ACHKW0_001734"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HFO7474581.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5228..6406)
                     /gene="setA"
                     /locus_tag="ACHKW0_001735"
     CDS             complement(5228..6406)
                     /gene="setA"
                     /locus_tag="ACHKW0_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HFO7474582.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6508..6639)
                     /gene="sgrT"
                     /locus_tag="ACHKW0_001736"
     CDS             complement(6508..6639)
                     /gene="sgrT"
                     /locus_tag="ACHKW0_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046325 - negative regulation of glucose
                     import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HFO7474583.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEELMQWDDRNSES"
     gene            6728..8383
                     /gene="sgrR"
                     /locus_tag="ACHKW0_001737"
     CDS             6728..8383
                     /gene="sgrR"
                     /locus_tag="ACHKW0_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HFO7474584.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLILTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8417..8516
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8547..9530
                     /gene="thiB"
                     /locus_tag="ACHKW0_001738"
     CDS             8547..9530
                     /gene="thiB"
                     /locus_tag="ACHKW0_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HFO7474585.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9506..11116
                     /gene="thiP"
                     /locus_tag="ACHKW0_001739"
     CDS             9506..11116
                     /gene="thiP"
                     /locus_tag="ACHKW0_001739"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HFO7474586.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11100..11798
                     /gene="thiQ"
                     /locus_tag="ACHKW0_001740"
     CDS             11100..11798
                     /gene="thiQ"
                     /locus_tag="ACHKW0_001740"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HFO7474587.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11912..12676)
                     /gene="yabI"
                     /locus_tag="ACHKW0_001741"
     CDS             complement(11912..12676)
                     /gene="yabI"
                     /locus_tag="ACHKW0_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HFO7474588.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQNGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGG"
     gene            complement(12762..13640)
                     /gene="araC"
                     /locus_tag="ACHKW0_001742"
     CDS             complement(12762..13640)
                     /gene="araC"
                     /locus_tag="ACHKW0_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HFO7474589.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            13979..15679
                     /gene="araB"
                     /locus_tag="ACHKW0_001743"
     CDS             13979..15679
                     /gene="araB"
                     /locus_tag="ACHKW0_001743"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HFO7474590.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPLLNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPCSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVPTL
                     "
     gene            15690..17192
                     /gene="araA"
                     /locus_tag="ACHKW0_001744"
     CDS             15690..17192
                     /gene="araA"
                     /locus_tag="ACHKW0_001744"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HFO7474591.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17392..18087
                     /gene="araD"
                     /locus_tag="ACHKW0_001745"
     CDS             17392..18087
                     /gene="araD"
                     /locus_tag="ACHKW0_001745"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7474592.1"
                     /translation="MLEDLKRLVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIATGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18162..20513
                     /gene="polB"
                     /locus_tag="ACHKW0_001746"
     CDS             18162..20513
                     /gene="polB"
                     /locus_tag="ACHKW0_001746"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HFO7474593.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20678..23584
                     /gene="rapA"
                     /locus_tag="ACHKW0_001747"
     CDS             20678..23584
                     /gene="rapA"
                     /locus_tag="ACHKW0_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HFO7474594.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23596..24255
                     /gene="rluA"
                     /locus_tag="ACHKW0_001748"
     CDS             23596..24255
                     /gene="rluA"
                     /locus_tag="ACHKW0_001748"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HFO7474595.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24372..25187)
                     /gene="djlA"
                     /locus_tag="ACHKW0_001749"
     CDS             complement(24372..25187)
                     /gene="djlA"
                     /locus_tag="ACHKW0_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HFO7474596.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25442..27796
                     /gene="lptD"
                     /locus_tag="ACHKW0_001750"
     CDS             25442..27796
                     /gene="lptD"
                     /locus_tag="ACHKW0_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HFO7474597.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27849..29135
                     /gene="surA"
                     /locus_tag="ACHKW0_001751"
     CDS             27849..29135
                     /gene="surA"
                     /locus_tag="ACHKW0_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0042277 - peptide binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA];
                     GO_process: GO:0060274 - maintenance of stationary phase
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HFO7474598.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29135..30124
                     /gene="pdxA"
                     /locus_tag="ACHKW0_001752"
     CDS             29135..30124
                     /gene="pdxA"
                     /locus_tag="ACHKW0_001752"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HFO7474599.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30121..30942
                     /gene="rsmA"
                     /locus_tag="ACHKW0_001753"
     CDS             30121..30942
                     /gene="rsmA"
                     /locus_tag="ACHKW0_001753"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HFO7474600.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30945..31322
                     /gene="apaG"
                     /locus_tag="ACHKW0_001754"
     CDS             30945..31322
                     /gene="apaG"
                     /locus_tag="ACHKW0_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HFO7474601.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31329..32171
                     /gene="apaH"
                     /locus_tag="ACHKW0_001755"
     CDS             31329..32171
                     /gene="apaH"
                     /locus_tag="ACHKW0_001755"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HFO7474602.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32249..32728)
                     /gene="folA"
                     /locus_tag="ACHKW0_001756"
     CDS             complement(32249..32728)
                     /gene="folA"
                     /locus_tag="ACHKW0_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HFO7474603.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32920..34782)
                     /gene="kefC"
                     /locus_tag="ACHKW0_001757"
     CDS             complement(32920..34782)
                     /gene="kefC"
                     /locus_tag="ACHKW0_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HFO7474604.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGFIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34775..35305)
                     /gene="kefF"
                     /locus_tag="ACHKW0_001758"
     CDS             complement(34775..35305)
                     /gene="kefF"
                     /locus_tag="ACHKW0_001758"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HFO7474605.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35413..36744)
                     /gene="yaaU"
                     /locus_tag="ACHKW0_001759"
     CDS             complement(35413..36744)
                     /gene="yaaU"
                     /locus_tag="ACHKW0_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474606.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36803..37090)
                     /gene="fixX"
                     /locus_tag="ACHKW0_001760"
     CDS             complement(36803..37090)
                     /gene="fixX"
                     /locus_tag="ACHKW0_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HFO7474607.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37087..38373)
                     /gene="fixC"
                     /locus_tag="ACHKW0_001761"
     CDS             complement(37087..38373)
                     /gene="fixC"
                     /locus_tag="ACHKW0_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7474608.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38424..39365)
                     /gene="fixB"
                     /locus_tag="ACHKW0_001762"
     CDS             complement(38424..39365)
                     /gene="fixB"
                     /locus_tag="ACHKW0_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HFO7474609.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39380..40150)
                     /gene="fixA"
                     /locus_tag="ACHKW0_001763"
     CDS             complement(39380..40150)
                     /gene="fixA"
                     /locus_tag="ACHKW0_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HFO7474610.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9646 a        11086 c        10589 g         8882 t
ORIGIN      
        1 caaatcaata tcaaaaaaaa atcgcaaaac atataattca atacaaatca tcaggatagg
       61 ttttgcaacg cgtgcatttt gtcccctttt tcctcgttga ttagatgcaa aaatttatgc
      121 tgaaatatgt caaccgatga aaagcgtcgg tagttaagca gaaattaata tcgcttactt
      181 taaccaccgc agcacaatta gctaatttta cgggtgcaga actcacgctg gcgggacgtt
      241 tttattgcgt cagggttgac atccgttttt gtatccagta actctaaaag catatcgcat
      301 tcatctggag ctgatttaat gactcacatc gttcgcttta tcggtctact actactaaac
      361 gcatcttctt tgcgcggtag acgagtgagc ggcatccagc attaagccag cacgcagtca
      421 aacaaaaaac ccgcgccatt gcgcgggttt ttttatgccc gaagcgaggc gctctaaaag
      481 agacaaggac ccaaaccatg agccagcaag tcattatttt cgataccaca ttgcgcgacg
      541 gtgaacaggc gttacaggca agcttgagtg tgaaagaaaa actgcaaatt gcgctggccc
      601 ttgagcgtat gggtgttgac gtgatggaag tcggtttccc cgtctcttcg ccgggcgatt
      661 ttgaatcggt gcaaaccatc gcccgccagg ttaaaaacag ccgcgtatgt gcgttagctc
      721 gctgcgtgga aaaagatatc gacgtggcgg ccgaatccct gaaagtcgcc gaagccttcc
      781 gtattcatac ctttattgct acctccccaa tgcacatcgc caccaagctg cgcagcaccc
      841 tggacgaggt gatcgaacgc gctatctata tggtgaaacg cgcccgtaat tacaccgatg
      901 atgttgaatt ttcttgcgaa gatgccgggc gtacacccat tgccgatctg gcgcgagtgg
      961 tcgaagcggc gattaatgcc ggtgccacca ccatcaacat tccggacacc gtgggctaca
     1021 ccatgccgtt tgagttcgcc ggaatcatca gcggcctgta tgaacgcgta cctaacatcg
     1081 acaaagccat tatttccgta catacccacg acgatttagg cctggcggtc ggcaactcac
     1141 tggcggcggt acatgccggt gcacgccagg tggaaggtgc aatgaacggg atcggcgagc
     1201 gtgccggtaa ctgttcgctg gaagaagtca tcatggcgat taaagttcgt aaggatattc
     1261 tcaacgtcca caccgccatt aatcaccagg aaatatggcg caccagccag ttagttagcc
     1321 agatttgtaa tatgccgatc ccggcaaaca aagccattgt tggcagcggc gcattcgcac
     1381 actcctccgg tatccaccag gatggcgtgc tgaaaaaccg cgaaaactac gaaatcatga
     1441 caccagaatc tattggtctg aaccaaatcc agctgaatct gacctctcgt tcggggcgtg
     1501 cggcggtgaa acatcgcatg gacgagatgg ggtataaaga aagtgaatat aatttagaca
     1561 atttgtacga cgccttcctg aagctggcgg acaaaaaagg tcaggtgttt gattacgatc
     1621 tggaggcgct ggccttcatc ggtaagcagc aagaagagcc ggagcatttc cgtctggatt
     1681 acttcagcgt gcagtctggc tctaacgata tcgccaccgc cgccgtcaaa ctggcctgtg
     1741 gcgaagaagt caaagcagaa gccgccaacg gtaacggtcc ggtcgatgcc gtctatcagg
     1801 caattaaccg catcactgac tataacgtcg aactggtgaa atacagcctg accgccaaag
     1861 gtcacggtaa agatgcgctg ggtcaggtgg atattgtcgc caactacaac ggtcgccgct
     1921 tccacggcgt cggcctggcc accgatattg tcgagtcctc cgccaaagcc atggtgcacg
     1981 tactgaacaa tatctggcgt gccgcagaag tcgaaaaaga gttgcaacgc aaagctcaac
     2041 acaacgaaaa caacaaggaa accgtgtgat gtcgaagaat taccatattg ccgtattgcc
     2101 gggggacggt attggtccgg aagtgatgac ccaggcgctg aaagtgctgg atgccgtgcg
     2161 caaccgcttt gcgatgcgca tcaccaccag ccattacgat gtaggcggcg cagccattga
     2221 taaccacggg caaccactgc cgcctgcgac ggttgaaggt tgtgagcaag ccgatgccgt
     2281 gctgtttggc tcggtaggcg gtccgaaatg ggaacattta ccaccagacc agcaaccaga
     2341 acgcggcgcg ctgttgcctt tgcgtaagca cttcaaatta ttcagcaacc tgcgcccggc
     2401 aaaactgtat caggggctgg aagcattctg tccgctgcgt gcagacattg ccgctaacgg
     2461 cttcgacatc ctgtgcgtgc gcgaactgac cggcggcatc tatttcggtc agccaaaagg
     2521 ccgcgaaggt agcggacaat atgaaaaagc gtttgatacc gaggtgtatc accgttttga
     2581 gatcgagcgt attgcccgca tcgcgtttga atctgctcgt aaacgtcgcc acaaagtcac
     2641 ctcaatcgac aaagctaacg tgctgcaatc ctctatttta tggcgtgaaa tcgtcaacga
     2701 gatcgccacg gaatacccgg atgtcgaact ggcgcatatg tacatcgaca acgccaccat
     2761 gcagctgatt aaagatccat cacagtttga cgttctgctg tgctccaacc tgtttggcga
     2821 cattctgtct gacgagtgcg caatgatcac tggctcgatg gggatgttgc cttccgccag
     2881 cctgaacgag caaggttttg gtctgtatga accggcaggc ggctcagcac cagatatcgc
     2941 aggcaaaaac atcgccaacc cgattgcgca aattctgtcg ctggcactgc tgctgcgcta
     3001 cagcctggat gccgatgatg cggcttgcgc cattgaacgc gccattaacc gcgcattaga
     3061 agaaggcatt cgcaccgggg atttagcccg tggcgctgcc gccgttagta ccgatgaaat
     3121 gggcgatatc attgcccgct atgtggcaga aggggtgtaa tcatggctaa aacgttatac
     3181 gaaaaattat tcgacgctca cgtagtgtac gaagccgaaa acgaaacccc gctgttatat
     3241 atcgaccgcc acctggtgca tgaagtgacc tcaccgcagg cgttcgatgg tctgcgcgcc
     3301 cacggtcgcc cggtacgtca gccgggcaaa accttcgcca ctatggatca caacgtctct
     3361 acccagacga aagacattaa tgcctgtggt gaaatggcgc gcatccagat gcaggagctg
     3421 attaaaaact gcaaagaatt tggcgtcgaa ctgtatgacc tgaatcaccc gtatcagggg
     3481 atcgtccacg taatggggcc ggaacagggc gtcaccttgc cggggatgac cattgtctgc
     3541 ggcgactcgc ataccgccac ccacggcgcg tttggcgcac tggcctttgg tatcggcact
     3601 tccgaagttg aacacgtact ggcaacgcaa accctgaaac agggccgcgc aaaaaccatg
     3661 aaaattgaag tccagggcaa agccgcgccg ggcattaccg caaaagatat cgtgctggca
     3721 attatcggta aaaccggtag cgcaggcggc accgggcatg tggtggagtt ttgcggcgaa
     3781 gcaatccgtg atttaagcat ggaaggtcgt atgaccctgt gcaatatggc aatcgaaatg
     3841 ggcgcaaaag ccggtctggt tgcaccggac gaaaccacct ttaactatgt caaaggccgt
     3901 ttgcatgcgc cgaaaggcaa agatttcgac gacgccgttg cctactggaa aaccctgcaa
     3961 accgacgaag gcgcaacttt cgataccgtt gtcactctgc aagcagaaga aatttcaccg
     4021 caggtcacct ggggcaccaa tccaggccag gtgatttccg tgaacgacaa tattcccgat
     4081 ccggcttcgt ttgccgatcc ggttgaacgc gcgtcggcag aaaaagcgct ggcctatatg
     4141 gggctgaaac cgggcattcc gctgaccgaa gtggctatcg acaaagtgtt tatcggttcc
     4201 tgtaccaact cgcgcattga agatttacgc gcggcagcgg agatcgccaa agggcgaaaa
     4261 gtcgcgccag gcgtgcaggc actggtggtt cccggctctg gcccggtaaa agcccaggcg
     4321 gaagcggaag gtctggataa aatctttatt gaagccggtt ttgaatggcg cttgcctggc
     4381 tgctcaatgt gtctggcgat gaacaacgac cgtctgaatc cgggcgaacg ttgtgcatcc
     4441 accagcaacc gtaactttga aggccgccag gggcgcggcg ggcgcacgca tctggtcagc
     4501 ccggcaatgg ctgccgctgc tgctgtgacc ggacatttcg ccgacattcg caacattaaa
     4561 taaggagcac accatggcag agaaatttat caaacacaca ggcctggtgg ttccgctgga
     4621 tgccgccaat gtcgataccg atgcaatcat cccgaaacag tttttgcaga aagtgacccg
     4681 tacgggtttt ggcgcgcatc tgtttaacga ctggcgtttt ctcgatgaaa aaggccaaca
     4741 gccaaacccg gacttcgtgc tgaacttccc gcagtatcag ggcgcttcca ttttgctggc
     4801 acgagaaaac ttcggctgtg gctcctcgcg tgagcacgcg ccctgggcat tgaccgacta
     4861 cggttttaaa gtggtgattg cgccgagttt tgctgacatc ttctacggca atagctttaa
     4921 caaccagctg ctgccggtga aattaagcga tgcagaagtg gacgaactgt ttgcgctggt
     4981 gaaagccaat ccggggatcc atttcgacgt ggatctggaa gcgcaagagg tgaaagcggg
     5041 agagaaaacc tatcgcttta ccatcgatgc cttccgccgc cactgcatga tgaacggtct
     5101 ggacagtatt gggcttacct tgcagcacga cgacgccatt gccgcttatg aagcaaaaca
     5161 acctgcgttt atgaattaat ccccttgccc ggtcaaatga ccgggctttc cgctatcgtc
     5221 cacgtcatca aacgtcttta acctttgcgg ttaaaaataa tgcgacaaca gaaataaccg
     5281 caattaccca gtagacagca aagtgccccc aactttgtgc aattgctccc tgaataacgc
     5341 cagccagaat taccccggta gaaatactgt tagtaaataa ggtggtagct gcccccgctc
     5401 ttccaggcat taaatcctga aaccatagca tcccaatacc cgcaacaatg ccgataaata
     5461 cagcgttaaa aagttgcagc gtcatcaacg ccatacggct atgaaagaaa atcaatccgg
     5521 tgtaaaacag tactcctgcc gccactgcta tgaccatcat tcgccgctta ccataacgtt
     5581 tgacatagta gccagccaga atcattgctg gtatttccag tccagctgcc gtccccatca
     5641 ggaaacccgc cagtttgtct ggcaatccta actcgctact gatccacaac ggcatatcaa
     5701 taatgtacat ggtgttgcag gtccacatta acgtcgaggc gacaaataac atccgtacgt
     5761 tactatcctg ccagccacct tgcattgata aagcattttc cgacggcagt tctacccgcg
     5821 ccacagacgg aagcataaat gcaatcaata ccagactgag tgtgaatatc ccggcggcaa
     5881 tcgaaaacat caccgtaaag ccgtaattca acgccagcat aaaggccaac ggtggaccga
     5941 taacccatgc cagagaaagc tgcgcacgca tcaccgagct aaacatcacc acttctcgcg
     6001 ccgagttatc cgcatattcc cgcgccagag caaataactg tggcattgcc gtattggcca
     6061 gagatgccag aagcacacca caggtgataa gcgtcagata atgacgatta aatgcaaaca
     6121 atagcgcatt gccgatagcc atcaaacagc aaaatataat cagttttcgc cgatcgccct
     6181 gactgtcaga acgttttgcc aaccagaggc ttacgccgat cccagcaata gcattcaccg
     6241 tataaaagag tccgatccag aaaggttgcg cgccaacctc acgactcaga aataagctca
     6301 atgtaggagc ctgtagcgcc ccggccaccc ccatcataaa agcgaccagc ataaatgccg
     6361 cgtaaacacc gttcatacgg cgagccatcg tcattatcca gatcatacgt tcccttttta
     6421 gcgcggcgag aataaaaaaa accagcaggt ataatctgct ggcgggtgat tttacaccaa
     6481 tactcagtca cacatgatgc aggcaagtca actttcagaa ttgcggtcat cccactgcat
     6541 cagttcttca agcatggtta atcgttgtgg gacgcttaac caacgcaacc agcacaactt
     6601 cgctgtcgcg gtaaaatagt gctgataaaa ctgacgcatg gggcacctcc ttgcttcatc
     6661 gttaggaatt attgacttaa tatagggaaa ataaaattgc tgtcttttgc gcaggagttc
     6721 cccttttatg ccatctgctc gtctgcaaca acagttcatc cgcctgtggc aatgctgcga
     6781 gggtaaatcg caggacacaa cgctcaacga actggcagcg ttattgagct gctcgcgtcg
     6841 tcatatgcgt accctgctca acaccatgca ggatcgcggc tggctgacgt gggaagcgga
     6901 agtcgggcgc ggtaaacgct cgcgtctgac attcctctat accgggctgg cgcttcagca
     6961 acagcgggcg gaagacctgc tggagcagga tcgtatcgac caactagtgc agttggttgg
     7021 cgacaaagcg actgtgcggc aaatgctggt ttctcatctg ggccgcagct tccgccaggg
     7081 gcggcacatc ctgcgcgtgc tctactatcg tccgttgcgt aatctgctac ctggcagcgc
     7141 attgcgccgt tccgaaaccc atattgcccg gcaaatcttc agttcgctaa cgcgcataaa
     7201 tgaggaaaat ggggaactgg aagcagacat cgcccaccac tggcagcaaa tatcaccgct
     7261 tcactggcgt ttctttttgc gtccaggagt ccattttcat catggtcgtg aactggaaat
     7321 ggacgacgtg atcgcctctt taaaacgaat caatacgctg ccgctctatt cgcatattgc
     7381 ggacattgtg tcgccgacgc cctggacgct ggatatccat ctcacgcaac cggaccgctg
     7441 gttaccgtta ctgctgggac aagttccggc gatgatcctg ccgcgcgaat gggaaaccct
     7501 cagtaacttt gccagccatc ccatcggcac cggtccgtat gcggtgatcc gcaacaccac
     7561 caatcaactg aaaattcagg cattcgatga cttcttcggt taccgggcat taatcgatga
     7621 agttaacgtc tgggttctgc cggaaattgc cgacgagcca gctggaggtc tgatgctaaa
     7681 aggtccacag ggtgaggaaa aagagattga aagccgcctg gaggaaggtt gctactattt
     7741 actgttcgac agccgcaccc atcgcggggc gaatcagcaa gtcagggact gggtaagcta
     7801 tgtgctttct ccaactaatc tggtctattt cgctgaggaa cagtaccagc aactgtggtt
     7861 cccggcttat ggactgctcc cccgttggca ccatgcccgc accataaaga gcgaaaaacc
     7921 ggctggcctg gaaagcctca tcctgacctt ttatcaggat cacagtgagc atcgggtgat
     7981 tgccgggatc atgcagcaga ttctggcaag tcaccaggtc acgctggaaa tcaaagagat
     8041 cagctacgat cagtggcatg aaggagagat cgagagcgat atctggctta acagcgccaa
     8101 ctttacgctg ccgctggatt tttcgctgtt cgcgcacttg tgcgaggtgc cgctgctcca
     8161 acactgtatt ccgatcgact ggcaagccga cgccgcccgc tggcgcaatg gcgaaatgaa
     8221 cctggcgaac tggtgccagc aactggtcgc cagtaaagca atggtgccgc ttatccacca
     8281 ctggctgatc attcagggac aacgcagtat gcgcggcctg cgtatgaata ccctcggctg
     8341 gttcgatttt aaatcagcgt ggtttgcgcc gccggatcca tgattgctgg tagataacaa
     8401 aatcactaca ctaacgccgt tctcaacggg gtgccacgca aacgcgtgcg ctgagaaaat
     8461 acccgtcgaa cctgatccgg ataacgccgg cgaagggatt tgaggctcct tctcaagtcc
     8521 tttgccactc ttttttgagg tgcaaagtgt taaaaaaatg tcttcccctg ctgttgctgt
     8581 gcacagcgcc cgttttcgct aaacccgttc tgactgttta tacctacgat tccttcgccg
     8641 ccgactgggg gcctggtcca aagatcaaaa aagcttttga agccgactgt aactgcgaac
     8701 tgaaactggt ggcgctggaa gatggcgttt cgcttctcaa ccgtctacgg atggaaggca
     8761 aaaatagtaa agccgatgtg gtgctggggc tggataacaa tctgttagac gccgccagta
     8821 aaaccggact atttgccaaa agcggtgtgg cagcggatgc cgttaacgtt cccggcggct
     8881 ggaataatga cactttcgtc ccgtttgatt acggctattt cgccttcgtc tatgacaaga
     8941 acaaactgaa aaacccgccg caaagcctga aagaattggt tgagagcgat cagaactggc
     9001 gggtgattta tcaggatccg cgcaccagta cgccggggct gggcctgctg ttgtggatgc
     9061 aaaaagtcta tggcgatgac gccccacaag cctggcaaaa actggcgaag aaaaccgtca
     9121 cggtcaccaa aggctggagc gaagcctacg gcctgttttt aaaaggtgaa agcgatctgg
     9181 tactgagtta caccacctct ccggcttatc acattctcga agagaaaaaa gataactacg
     9241 ccgccgcgaa ctttagcgaa ggtcactatc tgcaggtgga agtcgccgcc cgcaccgctg
     9301 ccagcaagca gccggagctg gcgcaaaaat tcctccagtt tatggtttct ccggcttttc
     9361 agaatgcgat cccaaccggt aactggatgt atccggtggc aaatgtcacg ctgcctgccg
     9421 ggtttgaaca attgaccaaa ccggccacca cgctggagtt cacaccagct gaagtggcgg
     9481 cacaacgtca ggcatggatt agcgaatggc aacgcgccgt cagccgttaa ttcccggctg
     9541 gttaattcca ggtgtaagcg ccgccacgct ggtggtagcg gttgcgctcg cggcgtttct
     9601 cgccctgtgg tggaacgcgc cgcagggtaa ctgggtggca gtctggcagg acagctacct
     9661 gtggcatgtg gtgcgcttct ccttctggca ggcgtttctc tcggcgctac tctctgtcgt
     9721 acccgcgata ttcctcgccc gcgccctcta tcgcaggcgc tttccgggtc ggctggcgct
     9781 gttgcgtctg tgcgcaatga ccttgatcct cccggtgcta gttgctgttt tcggcattct
     9841 tagcgtctat ggtcgtcagg gctggctggc atcgctctgc caatcgctcg gtctggagtg
     9901 gaccttttcg ccctacggcc tgcaaggcat tttgctggcg cacgtatttt ttaatctgcc
     9961 gatggcgagc cgcttattac tccaggcact ggaaaacatt cccggcgaac aacgtcagct
    10021 tgccgcccag cttgggatgc gcggctggca ttttttccgc ttcgtcgaat ggccgtggct
    10081 acggcgacaa atcccgccgg ttgccgcgct tatctttatg ctctgtttcg ccagcttcgc
    10141 caccgtgcta tcgctgggcg gcggtccgca ggcaactact atcgagctgg ctatttatca
    10201 ggcgctgagt tacgactacg atcctgcccg cgcggcgatg ctggcgctgc tccagatggt
    10261 gtgttgcctt gggctggtgc tgttgagtca gcgactaagt aaggccattg cgcccggtac
    10321 cacgctgctg caaggctggc gcgatccgga cgatcgtctg catagccgca tttgcgacac
    10381 ggtgttaata gtgctggcgc tgctgctgtt gctgccaccg ttgctggcgg tgatcgtcga
    10441 tggggtaaat cgccagttgc cggaagtgct ggcacaaccg gtgctgtggc aggcgctgtg
    10501 gacctcgttg cgtattgcgc tggcggcagg tgtattgtgc gtagtgctga ccatgatgct
    10561 gctatggagc agtcgcgaac tgcgggcgcg gcagaaaatg ctggcgggtc aggcgctgga
    10621 gatgagcggc atgttgatcc tcgccatgcc ggggattgtg ctggctaccg gcttcttttt
    10681 actgctcaac aacactatcg gcctgccaca atctgctgac ggcattgtga ttttcaccaa
    10741 tgcgttaatg gcgatccctt atgcgctgaa agtactggaa aacccgatgc gcgatatcac
    10801 cgcccgctac agtatgttgt gtcagtcgct gggcattgaa ggctggtcgc gcttaaaagt
    10861 ggtcgagctg cgcgccctga aacgtccact ggcgcaggcg ctggcttttg cctgcgtgct
    10921 gtcgattggt gattttggcg tagtggcgtt gttcggtaac gatgatttcc gtaccctgcc
    10981 gttttatctc taccagcaaa ttggctccta tcgcagccag gacggcgcgg tcaccgcgtt
    11041 aattctgcta ctactctgtt ttctgctgtt taccgtgatt gaaaaactac cggggcgaaa
    11101 tgttaaaact gactgatatc acctggcttt atcaccattt gccgatgcgt tttagcttaa
    11161 cggtagaacg cggcgagcag gtggcaatcc tcgggccaag cggcgcgggt aaaagtaccc
    11221 tgctgaattt gattgccggt tttctgacgc cagccagcgg ttcgctgact atcgacagcg
    11281 ttgatcacac cacgacgccg ccgtcacgcc gtccggtatc gatgctgttt caggagaaca
    11341 acctgttcag ccacctgacg gtcgcgcaga acatcgggct ggggcttaat ccgggattga
    11401 agctgaacgc ggcgcagcag gagaaaatgc acgctatcgc ccgccagatg gggattgata
    11461 atttaatggc gcggttaccg ggcgagcttt ccggcggtca gcgacagcgc gtggcgttag
    11521 cgcgttgtct ggtgcgcgaa cagccgattt tattgctcga tgaaccgttc tctgcgctcg
    11581 atccggcgtt acgtcaggag atgctgacgc tggtgagcac gagctgccag cagcaaaaaa
    11641 tgacgctatt gatggtgtcg cacagtgtgg aagatgcggc gcggatcgcc acacgctcgg
    11701 tggtggtcgc cgacgggcgc atcgcctggc agggtaagac caatgagttg ttgagcggta
    11761 aggcgagtgc ttcggcacta ttggggatta cgggttagta tgcactgtcg gatgcggcgt
    11821 gaacgcctta tccgacctac gggtagtgcg ttttgtaggc ctgataagac gcggtcagcg
    11881 tcgcatcagg caacccgcac aagactatct cctaaccccc aaccacttta cgcaaaatat
    11941 cgatatacac cggcatcaac gggtggcgaa ttaacaccac cagcgccacc acgccgatgg
    12001 cagaaattaa cggcgtcagc cacaacaaac ggccgcgcga caaataatga cttaaacgat
    12061 cggttgcttt accgctgcgc cataaccgcc agcacagcca gccaccaacc cacaaaaata
    12121 ccgccgtcgc cagcagcaac catttaaact cgccgttctg cattccggct ggaatatcga
    12181 tcgccgcgcc cgccagaatc ccaggcagga agtaaaacgg tggccacagc aggcagccga
    12241 taatattcgg cgtaatgaat tttgccaccg gcagatccag cattcccgcc accattggca
    12301 ccagcggacg cgtcgggcca acaaagcgac cgaccagaat ggtgaacatg ctgtgttgat
    12361 gcaacgcatg ttcagtttta tcgagtaatg ctttgttttt cttcagaaat gaccagcgat
    12421 gcaacggctt tttaaaacgc cagcccagcc agaaggagat ccagtcgccc agcagacaac
    12481 cgacaatccc tgccagccag gcgtgccaga aacttaactc gccgctgcca atcagcgctc
    12541 ccagccccgc catcagcacc gtaccgggta gaatcaaacc gaccagcgcc agcgactcca
    12601 gaaaggccac caacaccacc gccatcaatg aatacacggt ggattgggta ataaagtgtt
    12661 ccagcaatgc ttgcataatg tgcctgtcaa atggacgaag cagggattct gcaaacccta
    12721 tgctactccg tcaagccgtc aattgtctga ttcgttacca attatgacaa cttgacggct
    12781 acatcattca ctttttcttc acaaccggca cggaactcgc tcgggctggc cccggtgcat
    12841 tttttaaata ctcgcgagaa atagagttga tcgtcaaaac caacattgcg accgacggtg
    12901 gcgataggca tccgggtagt gctcaaaagc agcttcgcct gactaatgcg ttggtcctcg
    12961 cgccagctta agacgctaat ccctaactgc tggcggaaaa gatgtgacag acgcgacggc
    13021 gacaggcaaa catgctgtgc gacgctggcg atatcaaaat tgctgtctgc caggtgatcg
    13081 ctgatgtact gacaagcctc gcgtacacga ttatccatcg gtggatggag cgactcgtta
    13141 atcgcttcca tgcgccgcag taacaattgc tcaagcagat ttatcgccag cagctccgaa
    13201 tagcgccctt ccccttgccc ggcgttaatg atttgcccaa acaggtcgct gaaatgcggc
    13261 tggtgcgctt catccgggcg aaagaaaccc gtattggcaa atattgacgg ccagttaagc
    13321 cattcatgcc agtaggcgcg cggacgaaag taaacccact ggtgatacca ttcgcgagcc
    13381 tccggatgac gaccgtagtg atgaatctct cctggcggga acagcaaaat atcacccggt
    13441 cggcagacaa attctcgtcc ctgatttttc accaccccct gaccgcgaat ggtgagattg
    13501 agaatataac ctttcattcc cagcggtcgg tcgataaaaa aatcgagata accgttggcc
    13561 tcaatcggcg ttaaacccgc caccagatgg gcgttaaacg agtatcccgg cagcagggga
    13621 tcattttgcg cttcagccat acttttcata ctcccgccat tcagagaaga aaccaattgt
    13681 ccatattgca tcagacattg ccgtcactgc gtcttttact ggctcttctc gctaacccaa
    13741 ccggtaaccc cgcttattaa aagcattctg taacaaagcg ggaccaaagc catgacaaaa
    13801 acgcgtaaca aaagtgtcta taatcacggc agaaaagtcc acattgatta tttgcacggc
    13861 gtcacacttt gctatgccat agcattttta tccataagat tagcggatcc tacctgacgc
    13921 tttttatcgc aactctctac tgtttctcca tacccgtttt tttggatgga gtgaaacgat
    13981 ggcgattgca attggcctcg attttggcag tgattctgtg cgagctttgg cggtggactg
    14041 cgccagcggt gaagagatcg ccaccagcgt agagtggtat ccccgttggc aaaaagggca
    14101 attttgtgat gccccgaata accagttccg tcatcatccg cgtgactaca ttgagtcaat
    14161 ggaagcggca ctgaaaaccg tgcttgcaga gcttagcgtc gaacagcgcg cagctgtggt
    14221 cgggattggc gttgacagta ccggctcgac gcccgcaccg attgatgccg acggtaacgt
    14281 gctggcgctg cgcccggagt ttgccgaaaa cccgaacgcg atgttcgtat tgtggaaaga
    14341 ccacactgcg gttgaagaag cggaagagat tacccgtttg tgccacgcgc cgggcaacgt
    14401 tgactactcc cgctatattg gcggtattta ttccagcgaa tggttctggg caaaaatcct
    14461 gcatgtgact cgccaggaca gcgccgtggc gcaatctgcc gcatcgtgga ttgagctgtg
    14521 cgactgggtg ccagctctgc tttccggtac cacccgcccg caggatattc gtcgcggacg
    14581 ttgcagcgcc gggcataaat ctctgtggca cgaaagctgg ggcggcctgc cgccagccag
    14641 tttctttgat gagctggacc cgctcctcaa tcgccatttg ccttccccgc tgttcactga
    14701 cacctggact gccgatattc cggtgggcac cttatgcccg gaatgggcgc agcgtctcgg
    14761 cctgcctgaa agcgtggtga tttccggcgg cgcgtttgac tgccatatgg gcgcagttgg
    14821 cgcaggcgca cagcctaacg cactggtaaa agttatcggt acttccacct gcgacattct
    14881 gattgccgac aaacagagcg ttggcgagcg ggcagtgaaa ggtatttgcg gtcaggttga
    14941 tggcagcgtg gtgcctggat ttatcggtct ggaagcaggc caatcggcgt ttggtgatat
    15001 ctacgcctgg tttggtcgcg tactcggctg gccgctggaa cagcttgccg cccagcatcc
    15061 ggaactgaaa gcgcaaatca acgccagcca gaaacaactg cttccggcgc tgaccgaagc
    15121 atgggccaaa aatccgtctc tggatcacct gccggtggtg ctcgactggt ttaacggccg
    15181 ccgcacaccg aacgctaacc aacgcctgaa aggggtgatt accgatctta acctcgctac
    15241 cgacgctccg ctgctgttcg gcggtttgat tgctgccacc gcctttggcg cacgcgcaat
    15301 catggagtgc tttaccgatc aggggatcgc cgtcaataac gtgatggcgc tgggcggcat
    15361 cgcgcggaaa aaccaggtca ttatgcaggc ctgctgcgac gtgctgaatc gcccgctgca
    15421 aattgttgcc tctgaccagt gctgtgcgct cggtgcggcg atttttgctg ccgtcgccgc
    15481 gaaagtgcac gcagacatcc cctcagccca gcaaaaaatg gccagtgcgg tagagaaaac
    15541 cctgcaaccg tgcagcgaac aggcacaacg ctttgaacag ctttatcgcc gctatcagca
    15601 atgggcgatg agcgccgaac aacactatct tccaacttcc gccccggcac aggctgccca
    15661 ggccgttccg actctataag gacacgataa tgacgatttt tgataattat gaagtgtggt
    15721 ttgtcattgg cagccagcat ctgtatggcc cggaaaccct gcgtcaggtc acccaacatg
    15781 ccgagcacgt cgttaatgcg ctgaatacgg aagcgaaact gccctgcaaa ctggtgttga
    15841 aaccgctggg caccacgccg gatgaaatca ccgctatttg ccgcgacgcg aattacgacg
    15901 atcgttgcgc tggtctggtg gtgtggctgc acaccttctc cccggccaaa atgtggatca
    15961 acggcctgac catgctcaac aaaccgttgc tgcaattcca cacccagttt aacgcggcgc
    16021 tgccgtggga cagtatcgat atggacttta tgaacctgaa ccagactgct catggcggtc
    16081 gcgagttcgg cttcattggc gcgcgtatgc gtcagcaaca tgccgtcgtt accggtcact
    16141 ggcaggataa acaagcccat gagcgtatcg gctcctggat gcgtcaggcg gtctctaaac
    16201 aggatacccg tcatctgaaa gtctgccgat ttggcgataa catgcgtgaa gtggcggtca
    16261 ccgatggtga taaagttgcc gcacagatca agttcggttt ctccgtcaat acctgggcgg
    16321 ttggcgatct ggtgcaggtg gtgaactcca tcagcgacgg cgatgttaac gcgctggtcg
    16381 atgagtacga aagctgctac accatgacgc ctgcaacaca aatccacggc gaaaaacgac
    16441 agaacgtgct ggaagcggcg cgtattgagc tggggatgaa gcgtttcctg gaacaaggtg
    16501 gcttccacgc gttcaccacc acctttgaag atttgcacgg tctgaaacag cttccaggtc
    16561 tggccgtaca gcgtctgatg cagcagggtt acggctttgc gggcgaaggc gactggaaaa
    16621 ctgccgccct gcttcgcatc atgaaggtga tgtcaaccgg tctgcagggc ggtacctcct
    16681 ttatggagga ctacacctat cacttcgaga aaggcaatga cctggtgctc ggctcccata
    16741 tgctggaagt ctgcccgtcg attgcagtag aagagaaacc gatcctcgac gttcagcatc
    16801 tcggtattgg tggtaaggac gatcctgccc gcctgatctt caatacccaa accggtccag
    16861 cgattgttgc cagcctgatt gatctcggcg atcgttaccg tctgctggtt aactgtatcg
    16921 acacggtgaa aaccccgcac tccctgccga aactgccggt ggcgaatgcg ctgtggaaag
    16981 cgcaaccgga tctgccaact gcttccgaag cgtggatcct cgctggtggc gcgcaccata
    17041 ccgtcttcag ccatgcgctg aacctcaacg atatgcgcca gttcgccgag atgcacgaca
    17101 ttgaaatcac ggtgattgat aacgacaccc gcctgccagc gtttaaagac gcactgcgct
    17161 ggaacgaagt gtattacgga tttcgtcgct aagtagccac atcaggtatg taacgcctga
    17221 tgcgacgctg acgcgtctta tctggcctac gcgctgtgtt tttgtaggcc ggataagcaa
    17281 agcgcatccg gcattcaacg cctgatgcga cgctggcgcg tcttatcagg cctacgcgct
    17341 gcgattttgt agaccggata agcaaagtgc atccggcacg aaggagtcaa catgttagaa
    17401 gatctcaaac gcctggtatt agaagccaac ctggcgctgc caaaacacaa cctggtcacg
    17461 ctcacatggg gcaacgtcag cgccgttgat cgcgagcgcg gcgtctttgt gatcaaacct
    17521 tccggcgtcg attacagcgt catgaccgct gacgatatgg tcgtggttag catcgcaacc
    17581 ggtgaagtgg ttgaaggtac gaaaaagccc tcctctgaca cgccaactca ccggctgctc
    17641 tatcaggcat tcccgtccat tggcggcatt gtgcatacgc actcgcgcca cgccaccatc
    17701 tgggcgcagg cgggtcagtc gattccagca accggcacca cccacgccga ctatttctac
    17761 ggcaccattc cctgcacccg caaaatgacc gacgcagaaa tcaacggtga atatgagtgg
    17821 gaaaccggta acgtcatcgt agaaaccttc gaaaaacagg gtatcgatgc agcgcaaatg
    17881 cccggcgtcc tggtccattc tcacggccca tttgcatggg gcaaaaatgc cgaagatgcg
    17941 gtgcataacg ccatcgtgct ggaagaggtc gcttatatgg ggatattctg ccgtcagtta
    18001 gcgccgcagt taccggatat gcagcaaacg ctgctggata aacactatct gcgtaagcat
    18061 ggcgcgaagg catattacgg gcagtaatga ctgtataaaa ccacagccaa tcaaacgaaa
    18121 ccaggctata atcaagcctg gttttttatt ggattttcaa cgtggcgcag gcaggtttta
    18181 tcttaacccg acactggcgg gataccccgc aagggacaga agtctccttc tggctggcga
    18241 cggacaacgg gccgttgcag gttacgcttg caccgcaaga gtccgtggcg tttattcctg
    18301 ccgatcaggt tccccgcgct cagcatattt tgcagggtga acaaggcttt cgcctgacac
    18361 cgctggcgtt aaaggatttt caccgccagc cggtgtatgg cctttactgt cgcgcccatc
    18421 gccaattgat gaattacgaa aagcgcctgc gtgaaggtgg cgttaccgtc tacgaggccg
    18481 atgtgcgccc gccagaacgc tatctgatgg agcggtttat cacctcaccg gtgtgggtcg
    18541 agggtgatat gcacaatggc actatcgtta atgcccgtct gaaaccgcat cccgactatc
    18601 gtccgccgct caagtgggtt tctatagata ttgaaaccac ccgccacggt gagctgtact
    18661 gcatcggcct ggaaggctgc gggcagcgca tcgtttatat gctggggccg gagaatggcg
    18721 acgcctcctc gcttgatttc gaactggaat acgtcgccag ccgcccgctg ctgctggaaa
    18781 aactcaacgc ctggtttgca aaccacgatc ccgatgtgat catcggctgg aacgtggtgc
    18841 agttcgatct gcgaatgctg caaaaacatg ccgagcgtta ccgtcttccg ctgcgtcttg
    18901 ggcgcgataa tagcgagctg gagtggcgcg agcacggctt taaaaacggc gtcttttttg
    18961 cccaggctaa aggtcggcta attatcgacg gtatcgaggc gctgaaatcc gcgttctgga
    19021 atttctcttc attctcgctg gaaactgtcg ctcaggagct attaggcgaa ggaaaatcta
    19081 tcgataaccc gtgggatcga atggacgaaa ttgaccgccg tttcgccgaa gataaacctg
    19141 cgctggcaac ttataacctg aaagattgcg agctggtgac gcagatcttc cacaaaactg
    19201 aaatcatgcc atttttactc gaacgagcaa cggtgaacgg cctgccggtg gatcggcacg
    19261 gcggttcggt ggcagcgttt ggtcatctct attttccgcg aatgcatcgc gctggttatg
    19321 tcgcgcctaa tctcggcgaa gtgccaccgc acgccagccc tggcggctac gtgatggatt
    19381 cacggccagg gctttatgat tcagtgctgg tgctggacta taaaagcctg tacccgtcga
    19441 tcatccgcac ctttctgatt gatcccgtcg ggctggttga aggcatggcg cagcctgatc
    19501 cagagcacag taccgaaggt tttctcgatg cctggttctc acgggagaaa cattgcctgc
    19561 cggagattgt gactaacatc tggcacgggc gcgatgaagc caaacgtcag ggtaataaac
    19621 cactttcgca ggcgctgaag atcattatga atgcctttta tggcgtgctt ggcaccaccg
    19681 cctgccgctt tttcgatccg cgcctggcat cgtcgatcac catgcgtggt catcagatca
    19741 tgcggcaaac caaagcattg attgaagcgc agggctacga cgttatctac ggagataccg
    19801 actcgacttt tgtctggcta aaaggtgcac attcggaaga agaggcggcg aaaatcggtc
    19861 gtgtactggt gcagcacgtt aacgcctggt gggcggaaac gctgcaaaaa caacggctga
    19921 ccagcgcatt agaactggag tatgaaactc atttctgccg ttttctgatg ccaaccattc
    19981 gcggagccga taccggcagt aaaaagcgct acgccgggct gattcaggag ggcgataaac
    20041 agcggatggt gtttaaaggg ctggaaaccg tgcgcaccga ctggacgccg ctggcccagc
    20101 agtttcagca ggagctatac ctgcgcatct tccgcaacga gccatatcag gaatatgtac
    20161 gcgaaaccat cgacaaactg atggcgggtg aactggatgc gcgactggtt taccgtaaac
    20221 gccttcgccg tccgctgagc gagtatcagc gtaatgtgcc gcctcatgta cgcgccgctc
    20281 gccttgccga tgaagaaaac caaaagcgcg gtcgcccctt gcaatatcag aatcgcggca
    20341 ccattaagta cgtatggacc accaacggcc cggagccgct ggactaccaa cgttcaccac
    20401 tggattacga acactatctg acccgccagc tacaacccgt ggcggaggga atactccctt
    20461 ttattgagga taattttgct acacttatga ccgggcaact tgggctattt tgagcaaaaa
    20521 aaagagttcg ccagatacca ttttgatgcg tgacgaatgc tttgccatcc agtaccatag
    20581 cgccctttcc attcctggac ctgaataaca ccactacctc ataagcacgg tagcgggtgg
    20641 ttattgcctg caattaaaga tatagagccg aacacatatg ccttttacac ttggtcaacg
    20701 ctggatcagc gatacagaaa gcgaattggg acttggaacc gttgtcgcgg tggatgcgcg
    20761 aactgtcact ttacttttcc catctactgg tgaaaaccgt ctgtacgcac gcagtgattc
    20821 ccccgtgacc cgcgtgatgt tcaaccctgg tgatactatt accagccatg acggctggca
    20881 gatgcaagtc gaagaagtaa aagaagaaaa tggcttgctg acctatatcg gtactcgcct
    20941 ggatactgaa gagtccggcg tagccctgcg tgaagttttc cttgatagca aactggtgtt
    21001 cagcaaaccg caggaccgtc tgtttgccgg gcagattgac cgtatggacc gctttgcgct
    21061 gcgttatcgc gcgcgtaagt attccagcga acagttccgt atgccgtaca gcggcctgcg
    21121 cggtcagcgt accagcctga ttccgcatca gctcaacatc gctcatgatg ttggtcgccg
    21181 ccacgcgccg cgcgtcctgc tggctgacga agtgggttta gggaaaacca ttgaagccgg
    21241 gatgatcctg catcagcaac tgctctctgg cgctgctgaa cgtgtgctga ttatcgtccc
    21301 ggaaacctta cagcatcagt ggctggtaga aatgctgcgc cgtttcaacc tgcgctttgc
    21361 actgtttgat gatgagcgtt atgccgaagc tcagcacgat gcttacaacc cgtttgacac
    21421 cgaacagctg gtgatttgct cgctggattt tgcccgtcgt agcaaacagc gcctggaaca
    21481 tctctgtgaa gccgaatggg acctgctggt ggtcgatgaa gcgcatcacc tggtgtggag
    21541 cgaagatgcg ccaagccgtg aatatcaggc cattgaacaa ctggcagagc acgtgccggg
    21601 cgttctgctg ctgaccgcga ccccggaaca gctggggatg gaaagccact tcgcccgtct
    21661 gcgtctgctg gacccgaacc gtttccacga ttttgcgcag ttcgttgaag agcagaaaaa
    21721 ttatcgtccg gttgcggacg ccgttgccat gctgctggca ggtaacaaac tgagcaatga
    21781 cgaactgaac atgctcggcg agatgatcgg cgagcaggat atcgagccgc tgttgcaggc
    21841 agcaaacagc gacagcgaag atgcccagag cgcccgtcag gagctggttt cgatgctgat
    21901 ggatcgccac ggcaccagcc gcgtgctgtt ccgtaacacg cgtaacggtg tgaaaggatt
    21961 cccgaaacgc gagctgcaca ccattaaact gccgctaccg acgcagtatc agacggctat
    22021 taaagtctcc ggcattatgg gcgcacgtaa aagtgcggaa gatcgtgctc gcgatatgct
    22081 ctacccggag cgtatttatc aggaatttga aggtgataac gccacctggt ggaacttcga
    22141 tccgcgcgtt gagtggctga tgggctacct gaccagccat cgctctcaga aagtgctggt
    22201 gatctgcgcc aaagctgcca ctgcgctgca actggagcag gtactgcgcg aacgtgaagg
    22261 tattcgcgct gcggtgttcc acgaaggtat gtcgattatc gaacgtgacc gcgctgccgc
    22321 ctggtttgcc gaagaagaca ccggcgcaca ggtactgctg tgctcagaaa tcggttctga
    22381 aggacgtaac ttccagttcg ccagccacat ggtgatgttt gacctgccat tcaacccgga
    22441 tctactggag cagcgtattg gtcgtctgga tcgtatcggc caggcgcacg atattcagat
    22501 ccatgtgcct tatctggaga aaaccgctca gtcggtgctg gtgcgctggt atcacgaagg
    22561 tctggatgca tttgagcaca cctgcccgac cggacgcact atttacgata gcgtatacaa
    22621 cgatctgatt aactatctgg cttcaccgga tcaaaccgaa ggctttgacg atctgatcaa
    22681 aaactgccgc gagcaacatg aagcgctgaa agcacagctg gaacagggtc gtgaccgcct
    22741 gctggaaatc cactccaacg gtggcgaaaa agcccaggca ctggcagaaa gcattgaaga
    22801 gcaggatgac gataccaacc tgatcgcctt cgccatgaac ctgttcgata ttatcggtat
    22861 caatcaggac gatcgcggcg acaacatgat cgtgctgacg ccgtccgatc atatgctggt
    22921 gccggacttc cctggcctgt cggaagatgg catcaccatc acctttgatc gtgaagtggc
    22981 actggcgcgt gaagatgcac agtttattac ctgggagcat ccgctgatcc gcaacggtct
    23041 ggatctgatc ctttctggcg ataccggtag cagcacgatt tcactgttaa aaaacaaagc
    23101 gttgccggta ggtacgctgt tggtggaact gatttatgtg gttgaagccc aggctccgaa
    23161 gcagttgcag ctcaaccgct tcctgccacc gacgccggta cgtatgctgc tggataaaaa
    23221 cggcaacaac ctggcggcgc aggtagagtt tgaaaccttt aaccgccagc ttaacgcggt
    23281 taaccgtcac accggcagca aactggttaa cgccgtgcag caggatgttc acgctatcct
    23341 tcaactgggt gaagcgcaga tcgagaaatc tgcccgtgca ttgattgatg cagcgcgtaa
    23401 cgaagccgac gaaaaactgt ctgccgagct gtctcgtctg gaagctctgc gtgcagtgaa
    23461 cccgaacatt cgtgacgacg aactgaccgc cattgagagc aaccgtcagc aggtaatgga
    23521 aagcctggat caggcaggct ggcgtctgga tgccctgcgt ttgatcgttg taacgcatca
    23581 gtaacggagc cgaaaatggg gatggaaaac tacaatccac cgcaggaacc ctggttggtt
    23641 atcctgtatc aggatgacca cattatggtg gttaacaagc cgagcggttt gttgtctgtg
    23701 ccgggtcgtc tggaagagca caaagatagc gtgatgacgc gcattcagcg tgattatccg
    23761 caggcagaat cggtgcatcg tctggatatg gctaccagcg gcgtgattgt agtggcgctg
    23821 accaaagccg cggagcggga gttaaaacgc cagttccgcg agcgcgagcc gaaaaagcag
    23881 tatgtggccc gcgtctgggg gcatccatcc cccgcagaag gtctggtgga tctgccgctg
    23941 atttgcgact ggccaaaccg cccgaaacag aaagtctgtt acgaaacggg taaacctgcg
    24001 cagacggaat atgaagtggt ggagtatgcg gcggataaca cggcaagagt ggtgttaaaa
    24061 ccgattaccg ggcgttcgca tcaattgcgt gtgcatatgc tggcgctggg tcatccgatt
    24121 ctcggcgatc gtttttatgc atcaccagaa gcgagagcga tggcaccacg tttgttgcta
    24181 catgcagaga tgttgacgat tacccatccg gcgtatggca atagtatgac gtttaaagcg
    24241 ccagcggatt tttaatcgtc acgctggcgc agttcggtag gcggataagg cgttcacgcc
    24301 gcatccggca attgtgcgcc gattgcctga tgcgacgcgg gccgcgtctt atcaggccta
    24361 cagtttaaaa cttatttaaa ccctttctgc tgctttatca gctcatatgc ctgctgaatt
    24421 tcctgcgctt tctgcttcgc catctccatc atctcaggcg gcaaaccttt cgccaccagc
    24481 ttatcgggat ggtgttcact catcagctta cggtaggcac gtttgatggt ggtcgcatca
    24541 tccgtcggct tcacgcccag cacattacag gcatcttcca gcgtcgggcc acgctgcgct
    24601 tgctgccagt taccaccgcc agattgctgc tgataaccgc cgccaaactg tgcaccgccc
    24661 tgcatcatgc gcaaaaactg gtcaaactgg gcgcgggaga tccctaattc ttcagcaatg
    24721 acatacagca ccgcccgttc attcgggtgc agtgaaccat cagcaaacgc cgcctgaatc
    24781 tggatctcca gaaacatacg aattaagtca aaacgaccaa agcagacact gcgaaactgg
    24841 cgcatctttt cgcgcagcgg gtaattatct gattttccca ctcggaacgc gttttgcgcc
    24901 gcagtacggg aagcgccatg aaggttcatt cggtccatca actggctggc gatatgaata
    24961 tcagcctccg tgacgcgacc tttggatttg gttaaatgcc ccatcacttc aaaagtggtg
    25021 gcaaaaaaca gcgcctgacg ctcacgctgg ttggcgaacc acgccatttt acggctacgg
    25081 gctttatcaa acatatggcc aattaacagg cctaacacta cgccccaaaa gccgccgccc
    25141 atcagtaagg ccacggccac gccaatgatt tttccccaat actgcatata ttccccaaat
    25201 cgacacacgg atatcagggc tatctcccac aatataaagg tgcttttacc gttttccggc
    25261 tgcggtcaat tgtgggacat cgcctataat ttgcattatc atacctgtca ttcaatgccg
    25321 tgcctaacac cactgacgct attcggacag gattaacact agcgtagaga tgacgagtac
    25381 gttagtctct gaccgtttgt cacgcgcaac gttaccgatg atggaacaat aaaatcaacg
    25441 tatgaaaaaa cgtatcccca ctctcctggc caccatgatt gccaccgccc tttatagtca
    25501 acagggactg gcagccgacc tcgcctcaca gtgcatgttg ggcgtgccaa gctatgaccg
    25561 tcctctggta cagggcgata ccaatgactt acccgtgact atcaatgctg accacgcgaa
    25621 aggggactac ccggatgacg ccgtgtttac tggcagcgtg gatatcatgc agggtaacag
    25681 ccgtctgcag gccgacgaag tgcagctcca tcaaaaagag gcaccaggac aaccggagcc
    25741 ggtacgtacc gttgatgcgc tcggtaatgt ccattacgac gataaccagg tgatcctcaa
    25801 agggccgaaa ggctgggcga atctgaacac caaagatacc aacgtctggg aaggtgatta
    25861 ccagatggtg ggtcgccagg gtcgcggtaa agcggacctg atgaaacaac gtggcgaaaa
    25921 ccgctatacc attctggata acggtagctt tacctcctgt ctgccgggtt ctgacacctg
    25981 gagcgtggta ggtagcgaaa ttattcatga ccgcgaagaa caagttgcgg agatctggaa
    26041 cgcccgcttt aaggtgggtc cggtaccgat cttttatagc ccctatttgc agttgccggt
    26101 gggtgacaaa cgtcgctctg gtttcttgat cccgaacgcc aagtacacca ccaccaacta
    26161 ctttgagttc tacctgccat attactggaa catcgcgcca aatatggatg ccaccatcac
    26221 gccgcattat atgcatcgtc gtggcaacat catgtgggag aacgaattcc gctacctctc
    26281 ccaggcgggc gctggcttga tggaactgga ctatctgcct tcagataaag tctatgaaga
    26341 tgaacacccg aacgatgaca gttcacgtcg ttggttattc tactggaacc actccggggt
    26401 catggatcag gtgtggcgtt tcaacgtcga ctacaccaag gtcagcgatc ctagctactt
    26461 caatgatttc gataacaagt acggttccag tactgacggc tacgcaacgc aaaaattcag
    26521 cgttggctat gcggtgcaaa acttcaatgc caccgtttca accaagcagt tccaggtttt
    26581 cagcgaacag aacaccagta gctactcggc agagccgcag ttagacgtta attactacca
    26641 gaatgatgtt ggtccgtttg atacgcgtat ttacggccag gcagtgcact ttgttaacac
    26701 cagagacgac atgcctgaag caacccgtgt tcacctggaa ccgaccatca atttgccgct
    26761 ctctaataac tggggcagca tcaataccga agcgaagttg ctggcaaccc attatcagca
    26821 aaccaatctt gactggtata actccagaaa cacgaccaag ctggacgaat ccgttaaccg
    26881 cgtaatgccg caattcaaag ttgacggcaa aatggtcttt gaacgcgata tggaaatgct
    26941 ggctccgggt tatacccaaa cgctggaacc gcgcgcgcag tatttgtacg tgccgtatcg
    27001 cgatcagagc gacatctata actacgactc gtctctgctg caatctgact actctggcct
    27061 gttccgggac cggacttacg gcggtcttga ccgtattgcc tccgctaacc aggtgacgac
    27121 cggtgtcaca tctcgcatat atgatgatgc tgccgttgaa cgttttaata tttccgttgg
    27181 tcaaatctac tatttcacgg agtctcgcac tggcgatgac aacataacat gggagaatga
    27241 cgacaaaacg ggttcactgg tgtgggcagg cgatacttac tggcgtatct ccgagcgttg
    27301 gggattgcgt ggcgggattc agtacgatac acgtctggat aacgtagcga ccagtaactc
    27361 cagcattgaa taccgtcggg atgaagaccg tctggtacag ctgaattacc gttacgccag
    27421 cccggaatat attcaggcta cgctgcctaa gtactattcc actgctgagc aatataagaa
    27481 tggtatttcg caggtaggtg ctgtcgccag ctggccaatt gccgatcgtt ggtccattgt
    27541 tggggcctac tactacgaca ccaatgcgaa caagcaagcc gactctatgt taggtgtgca
    27601 atacagctcc tgctgctatg caattcgcgt cggttacgag cggaagctga acggttggga
    27661 taacgataaa caacatgcgg tatatgacaa cgcaatcggc tttaacatcg aacttcgcgg
    27721 cctgagctcc aactacggtc tgggtacgca agagatgctg cgttcgaaca ttctgccgta
    27781 tcaaaacact ttgtgatttg ttgatttacc acgtaatccg cagtgcggtt aattgaaatg
    27841 gaaaaagtat gaagaactgg aaaacgctgc ttctcggtat cgccatgatc gcgaatacca
    27901 gtttcgctgc cccccaggta gtcgataaag tcgcagccgt cgtcaataac ggcgtcgtgc
    27961 tggaaagcga cgttgatgga ttaatgcagt cggtaaaact gaacgctgct caggcaaggc
    28021 agcaacttcc tgatgacgcg acgctgcgcc accaaatcat ggaacgtttg atcatggatc
    28081 aaatcatcct gcagatgggg cagaaaatgg gagtgaaaat ctccgatgag cagctggatc
    28141 aggcgattgc taacatcgcg aaacagaaca acatgacgct ggatcagatg cgcagccgtc
    28201 tggcttacga tggactgaac tacaacacct atcgtaacca gatccgcaaa gagatgatta
    28261 tctctgaagt gcgtaacaac gaggtgcgtc gtcgcatcac catcctgccg caggaagtcg
    28321 aatccctggc gcagcaggtg ggtaaccaaa acgacgccag cactgagctg aacctgagcc
    28381 acatcctgat cccgctgccg gaaaacccga cctctgatca ggtgaacgaa gcggaaagcc
    28441 aggcgcgcgc cattgtcgat caggcgcgta acggcgctga tttcggtaag ctggcgattg
    28501 ctcattctgc cgaccagcag gcgctgaacg gcggccagat gggctggggc cgtattcagg
    28561 agttgcccgg gatcttcgcc caggcattaa gcaccgcgaa gaaaggcgac attgttggcc
    28621 cgattcgttc cggcgttggc ttccatattc ttaaagttaa cgacctgcgc ggcgaaagca
    28681 aaaatatctc ggtgaccgaa gttcatgctc gccatattct gctgaaaccg tcgccgatca
    28741 tgactgacga acaggcccgt gtgaaactgg aacagattgc tgctgatatc aagagtggta
    28801 aaacgacttt tgctgccgca gcgaaagagt tctctcagga tccaggctct gctaaccagg
    28861 gcggtgatct cggctgggct acaccagata ttttcgatcc ggccttccgt gacgccctga
    28921 ctcgcctgaa caaaggtcaa atgagtgcac cggttcactc ttcattcggc tggcatttaa
    28981 tcgaactgct ggatacccgt aatgtcgata aaaccgacgc tgcgcagaaa gatcgtgcat
    29041 accgcatgct gatgaaccgt aagttctcgg aagaagcagc aagctggatg caggaacaac
    29101 gtgccagcgc ctacgttaaa atcctgagca actaatggtt aaaacccaac gtgttgtgat
    29161 cactcccggc gagcccgccg ggattggccc ggacttagtt gtccagcttg cacagcgtga
    29221 gtggccggtc gaactggttg tttgtgccga tgccactctc cttaccgacc gggcagcgat
    29281 gctcggtttg ccgctcaccc tccgccctta ttcccccaac tcccctgcac aaccgcaaac
    29341 tacgggcaca ttaacgctac ttcctgtcgc gctacgtgaa tctgtcactg cggggcagtt
    29401 agcgattgaa aatggacatt acgtggtgga gacgctggcg cgagcgtgtg atggctgtct
    29461 gaacggtgaa tttgctgcgc tgatcacagg ccccgtgcat aaaggcgtca ttaacgacgc
    29521 aggcattccg tttaccggtc ataccgagtt tttcgaagag cgttcgcagg cgaaaaaagt
    29581 ggtgatgatg ctggcgaccg aagaacttcg cgtggcgctg gcaacgacgc atttaccgct
    29641 gcgcgatatc gcagatgcta tcacccctgc acttttgcac gaagtgattg ctattttgca
    29701 tcacgatttg cgtacaaaat ttggtattgc cgaaccgcgc attctggtct gcgggctgaa
    29761 tccgcacgcg ggcgaaggcg gtcatatggg tacggaagag atagacacca ttattccggt
    29821 gctcgacgag ctgcgggcgc agggaatgaa actcaacggg ccgctgcctg ccgataccct
    29881 gtttcagccg aaatatctcg ataacgccga cgccgtgctg gcgatgtacc acgatcaggg
    29941 tcttcccgtg ctaaaatacc agggcttcgg gcgcggtgtg aacattacgc tgggcctgcc
    30001 ctttattcgc acatcagtgg accacggcac cgcgcttgaa ctggcgggac gtggcgaagc
    30061 cgatgtcggc agttttatta cggcgcttaa tctcgccatc aaaatgattg ttaacaccca
    30121 atgaataatc gagtccacca gggccactta gcccgtaaac gcttcgggca aaactttctc
    30181 aacgatcagt tcgtgatcga cagcattgtt tctgccatta acccgcaaaa gggccaggca
    30241 atggtcgaaa tcggccccgg tctggcggca ttgaccgagc cggtcggcga acgtctggac
    30301 cagctgacgg tcatcgaact tgaccgcgat ctggcggcac gtctgcaaac gcatccattc
    30361 ttaggcccga aactgacgat ttatcagcag gatgcgatga cctttaactt tggcgaactg
    30421 gccgagaaaa tgggtcagcc gctgcgtgtt ttcggcaacc tgccttataa catctccacg
    30481 ccgttgatgt tccatctgtt tagctatact gatgccattg ccgacatgca ctttatgttg
    30541 caaaaagagg tggtgaatcg tctggttgca ggaccgaaca gcaaagcgta tggtcgatta
    30601 agcgtcatgg cgcaatacta ttgcaacgtg atcccggtac tggaagttcc gccgtcagcc
    30661 tttacaccac cacccaaagt ggattccgcc gtcgtgcgcc tggttcctca tgcaacgatg
    30721 cctcacccgg ttaaagatgt tcgtgtgttg agccgcatca ccaccgaagc ctttaaccag
    30781 cgtcgtaaaa ccattcgtaa cagcctcggc aacctgttta gcgtcgaggt gttaacggga
    30841 atggggatcg acccggcgat gcgagcggaa aatatctctg tcgcgcaata ttgccagatg
    30901 gcgaactatc tggcggagaa cgcgcctttg caggagagtt aacgatgatc aattcgcccc
    30961 gagtgtgtat tcaggttcaa agcgtctaca ttgaggcaca atcttcacct gataatgaac
    31021 gttacgtttt tgcttatacc gtaaccatac gcaatctggg gcgagcgcca gtgcagttgt
    31081 tggggcgtta ctggctgatc accaatggca atggccgtga aaccgaagtc cagggcgaag
    31141 gagtggttgg cgtccagcca cttatcgcgc ctggcgaaga gtaccagtac accagcggtg
    31201 caatcattga aaccccgctg ggcaccatgc agggtcacta cgaaatgatc gatgaaaatg
    31261 gcgtcccttt cagcatcgac attcccgtat tccgactcgc cgttcccaca ctcattcatt
    31321 aaaagaatat ggcgacatac cttattggcg acgttcatgg ttgttacgat gaactgatcg
    31381 cattgctgca taaagtagaa tttacccctg ggaaagatac cctctggctg acgggtgatc
    31441 tggtcgcgcg cgggccgggt tcgctggatg ttctgcgcta tgtgaaatcc ttaggcgaca
    31501 gcgtacgtct ggtgctgggc aatcacgatc tgcatctgct ggcggtattt gccgggatca
    31561 gccgcaataa accgaaagat cgcctgacac cgctgctgga agcgccggat gccgacgagc
    31621 tgcttaactg gctgcgtcgc cagcctttgc tgcaaatcga cgaagagaaa aagttggtga
    31681 tggcccacgc agggatcacg ccgcagtggg atctacagac cgccaaagag tgcgcgcgcg
    31741 atgtagaagc ggtgctatcg agtgactcct atcccttctt tctcgacgct atgtacggcg
    31801 atatgccaaa taactggtca ccggaattgc gggggctggg aagactgcgt tttatcacca
    31861 acgcctttac ccgtatgcgt ttttgctttc caaacggtca actggatatg tacagcaaag
    31921 aatcgccgga agaagcccct gccccactga aaccgtggtt tgcgattcct ggccctgtcg
    31981 ctgaagaata cagcatcgcc tttggtcact gggcatcgct ggaaggcaaa ggtacgccgg
    32041 aaggtattta cgcgctggat accggctgct gctggggtgg gtcattaacc tgcctgcgct
    32101 gggaagataa gcagtatttt gtccagccgt cgaaccggca taaggatttg ggcgaagcgg
    32161 cggcgtctta aacacagcct gatataggaa ggccggataa gacgcgaccg gcgtcgcatc
    32221 cggcactagc cgtaaattct atacaaaatt accgccgctc cagaatctca aagcaatagc
    32281 tgtgagagtt ctgcgcatcg gcgtcgtgga attcgctgaa taccgattcc cagtcatccg
    32341 gctcgtaatc cgggaaatgg gtgtcgcctt ccacttctgc gtcgatatgc gtcagataca
    32401 gtttttgcgc ttttggcagg aactgctcat aaacgcgacc gccgccaatc accatgattt
    32461 ctggtacgtc accacacgcc gcgatggctt catccaccga cttcacccac gttacgcgat
    32521 cgtccgtacc cggttgactg ctgaggataa tatttttgcg tcctggcaac ggacgaccga
    32581 ttgattccca ggtatggcgg cccataatca cgggtttatt taaggtgttg cgtttaaacc
    32641 aggcgagatc ggcaggcagg ttccacggca tggcgttttc catgccgata acgcgatcta
    32701 ccgctaacgc cgcaatcaga ctgatcattg agatttcccg ataaaaaaaa ttgtcgccac
    32761 tatacgtaaa gcgtaaaccg tcgtcgactg gtgcgaggat gatgttgagg aaaattttat
    32821 attctgctgg cgagtccacg ctctctccct ggactcgccg cattacaatg aaacaaaaac
    32881 aaacagttag ctgtaaagtg tgatttacgt cactctttat taggatgagg gtttcgtttc
    32941 cggttcatcc gccatgttgc cggtatgttt accttcttcg gttccctgcc agccatgtcg
    33001 ttgaattaat gacagatgtt cgcgatcctc ggtaatgatc tcacttaaca tcgcgctggt
    33061 gcgtttatag accgccgcgc gggctttggt atcattctca accattgcca tctcttccac
    33121 catctgaata ttaaagcggc ggaacacatc ggcacgttct cgcgcttcat acggccccag
    33181 acctaaactt tccagtgcca gacgcccggt tttcagcgca ccttcgaagg tttcacgctc
    33241 cggcttttca acgcctgcct gacgcaaacg aatgtagtgg tcgacatcgc gggcgcgggc
    33301 aataatctgc aaatgcggga aatgttcttt caccatctct gtcagttgca ggttggtttg
    33361 cggatcgtcg atggcgttaa tcagcacttc cgctttcgcc gctccggcag attccagtaa
    33421 atccatccgc gtggcatcgc cataaaacac tttcatacca aatttacgca aggtttcgat
    33481 atggtccgga tcgtgatcga gtaccaccat tttcaccccg ctggagagca gtaaacgtcc
    33541 ggtaatctgc ccaaaacgac cgaatccggc gataatcacg cgcggctgtt cttcgtcgat
    33601 ctcatcggct tcacgcgctt cctcagtaga agattgctca aggcgattga ggatcaccag
    33661 cagaatcggc gttgctgcca tcgacagcgc caccgccagg gtcagcgatt tcgcccactc
    33721 cggctccagc acattcgcca tctgcgccgc gccaaatacc acaaaggcaa actcactgcc
    33781 ctgccctaac aacaccgcaa accaacgacg ctgtttattt ggcacttgca acggtcgggc
    33841 aatcagccac agcatggcga ttttgatgat gaggaaaccg agcagcaaaa tgacaatgcg
    33901 caatgggttt tcaagcagcg tgccaaagtc tatcgacatg ccaacaccga tgaaaaacag
    33961 ccccaacagc aaacctttaa atggttcgat atcgctctcc agcgcatgac ggtattccga
    34021 gcttgccagc agtacgcccg ccagaaacgc gcccatcgcc atcgacaagc cgacctcttc
    34081 cagcagcaaa ccaaagccaa acacgaggaa taacgccacg gcactaaaca cttcccgcaa
    34141 gccagagcgg gctacaaaac gcagcgccgg acgcgtgaca tagcgcccca gcaataccac
    34201 cagcaccagc gcacccgcca cttttaacgc cgagagagca aatgcgccca tcgtcgtcga
    34261 ggcactgctc gttgccagta gcggaatcat cgccaccagc gggatcgccg cgatatcctg
    34321 gaacagcagc accgcaaagg cactgcgacc catttgcgtc accatcagat tgcgttcatt
    34381 catcgcctgc atggcaatcg ccgtagagga gagcgccagc gtcatgccga tcaattccgc
    34441 gacctgccag cgcagcccaa gtaacatgca gaacagcccc agcaggccgc cgcaaatcac
    34501 catctgcaat gcgccaccgc cgaacactgc cgcacgcagc ttccacagcc tttgtggatc
    34561 gagttcgagg ccgataataa acagcatcag caccacgcca atctcggcaa agtgcagaat
    34621 agattcggca tcggtcacca gtcgcagccc ccacgggcca ataatgaagc cggcgatcag
    34681 gtagccaagt accgatccca gaccaagacg taccgcaatg ggtacaatca gcgctgccga
    34741 accgagataa atcagcgcct gaatcagcgt atggctatcc atgatgggcc tcctgccatt
    34801 ccagcagacg ttgcttatag tgacgcgcct gcccttcgag ggtttcgtcg tcacaaataa
    34861 aggtgcagtg catggcaaac ggtggcagcc agttcagccc gcagtagatt gccgtcgcct
    34921 gtagcggctg cgacagcaca tcaaagcccg gatgcgcacc aatttcaaaa tggctttccc
    34981 cgccgccggt cgtcaccgcc cacagcaaat gtttgccatg cagcgccgtg ccgccgtgac
    35041 cgtaagccca gccgtgcgag aaaactttat cgatccaaag tttgaggagc ggtggaatgc
    35101 tgtaccactg catcgggtgc tgccagacga tcagatcggc gcgagacagc gcctcctgct
    35161 cggcggcaat atcgatattg aagtcaggat agagttgata aagagagcga atttcgacgc
    35221 cttccagcgt ccttgcctgt tcaagcatcc gtttattcgc atgggaatga tgcggatacg
    35281 gatgcgcata aattataaga atcataggta gcccgcgtga ttacgccttt gttttcccac
    35341 aaagtgtagt catatattca cgcggcttat agtcaataat attgattcag aaaagagaga
    35401 aatctgaaca atttacccca ttctctgccc gcggatcgtc atattgctgg tttgcgccag
    35461 tgacatccct cgagtctccg gggcaaacgc tacggaaatc aacaagccaa acagcgagat
    35521 acccgccccc attagcatcg tgttactgat accgtaatta ttgataaaga tcggcagtgc
    35581 ccaggtcgaa acaatggtgc caatacggct taaggacata atcacgccca cggcagaggc
    35641 gcggatatct gtcgggaaga gttcattagg atagagccac tgcaaattac ccggcccgcc
    35701 agagaaaaag gcatacaccg caaaggccat cactaccagc cagatcccca tatccgggat
    35761 cagccccaaa accgccagcg ccagcgtcat catggcaaag ctgccaatca acaatggacg
    35821 ccgtccggca gtgtttaacc acagcatcgg cggaatacag ccgagcataa agaacaggct
    35881 aatcaccacg ttccccagcg ccgcgttttt gccaacaccc aatcccaaca aaccaacgat
    35941 ttgcgggcca aaggtgtaaa tggcgaacat tgggatcacc tggcaggtcc agatggcggc
    36001 aacaaacaga acaaaaggaa aatggcggcg attaaacaga tcgcgaaaac gggtttgctg
    36061 cggttgctct tcatcgaaag ccaccggttc gccaaacagt tttatcatca tctcttcgca
    36121 ctcttttact cgccctttgc gtaataacca gcggggagat tcaggcagtt cgaatcgacc
    36181 aatcaaaatc aacaaacagg ggatcgccgc gctacccagc atccagcgcc agccgccttc
    36241 cacatcataa agccagtagc cgaccagatc ggcgcaggtc gcgccgacat accacatggc
    36301 ggcgataaag ctgatggaaa acgcccgctg acgggtactg gagaactcgg tgatcattga
    36361 ggtggcgatg ggataatctg caccgatgac aatgccgata agtacccgca tcaccaacag
    36421 ttcgacgggg gatgaaacaa acatcgtcgc caccgatatc acgccgatgg cgatgatatc
    36481 aatgaggaac attttgcgcc gtccgacttt atcggaaata tagccaaaca gcgatgtgcc
    36541 aacgaacagc ccggcgagcg ttcccgcgcc caacaagcca atccagtcag cgtccagttt
    36601 tagcgccgga gtcagttgct ccagcgccac gccaatcatt accagtacat aaccatccag
    36661 aaacggacca ccgcttcccc acagcaaaat gcggcggtga attgaggaga atttgagatc
    36721 gtcaaagttt ctggacggtt gcatgggtta ttcctgtttt tttgcaatga gagtcagtac
    36781 ggcgttacgt atcaaaccaa catcagccgt aacggaactc cacaccaaag gtgccgcgcg
    36841 ggtattccca ctgttccagc gccgacccca gccccagaat gcgacaggtg ccgcactcca
    36901 gacatccggc gtaatcgaag cgcacactgc cgtcatcctg cttcttgtac agacctgcgg
    36961 ggcacgcttt caccagcagc tccagggcct gtttatcggc atcggccttc acaacaatgt
    37021 gcggatgctc ttcatcgaca ttgaatttat tgacgcccag tttgacgtcc acattgacgg
    37081 gagaagtcat aaaacggtca ctcctttcat gccatccttg atcagattga tgaagcccac
    37141 tttcttgccg tggcggagga ttttcttgcg catcagttcc ggcgcactgc cgtcaatggt
    37201 gaacaggtca cgcgccacgc ccaccgccag ttccgggtag ccgctaaaca tgcgtgggtt
    37261 atcaaggaag gccggtagtt tctggtacat acgcatatcg cgcagcgggc cactctcaag
    37321 atgctgacga tattccgcca gtttttgctt actgaaatcg tcgcttttca tcgctgaaag
    37381 cacggttttt gctgcggctt ccccggcggc aatcgccaga tccataccgc gaatggtaaa
    37441 accgaggttc atacacattc cggcggcatc accggcaatc aatacgccgt caccaaccaa
    37501 ctccggcagc atgttgatgc ctgcttccgg cactacgtga gcggaatatt ccaccagctt
    37561 gccacccgcg atcagcggtg caacggccgg atgttgtttg aaatcttcca gcatttgcgg
    37621 caccgatttt tttgcgtcat gcagatgatg cagaccacaa accagcccca gcgacagggt
    37681 attttcattg gtataaagga agccgccgcc catcaggcca tcggtgggtg atcccgcaaa
    37741 cagacaagcc gcgccctgat taccctgcaa ctgaaaacgg tcttcgatta ccgacttcgg
    37801 taactcgatc agttccttca cgccaaccgc cacatccgtc ggtttaacgc gttttgccat
    37861 ccccagcttt tcggcaagga tggagttcac tccatcagca aggatcaccg ttttcgcttc
    37921 aatcacatcg ccatcggctt ctacaccgac gactttgcca tcgcgctgta cgaggttatc
    37981 gacgcggatc ccggtaatta actgcgcgcc cgcttcttcg gcctgctcca tcagccaggc
    38041 atcaaattta ctgcgcaaaa cggagtaaga acgctgggat ggcgaggttt cgtcaccatt
    38101 gcagtagtcc atagtcatcg ctgacttttc cgtcataaac gcgagttttt catgggtgat
    38161 caggcgttct acgggggcgg agtcggcgaa accaggaata atgtgttcca ggctgtgggc
    38221 atagagacgc ccgccggtga cgttcttggc acctgcggaa ttgccacgct cgataactaa
    38281 cacttgcgcc ccttcgcggg cgagcaccag tgcggcaacc gaaccggcaa gcccagcacc
    38341 gacgatgatg gcgtcaaaga tatcttcgga cataagagct ccctgtcagc ggcagggcca
    38401 aaatgcagcc ctgccagagt ggatcaacgc gctaaagctg cggtcagcgc cggaaggatc
    38461 ttcacggcgt caccaacaat gccgtaatcc gcgtactgga agatcggcgc atttttatct
    38521 ttattgatgg cgaaaatggt ttgcgatgca ttagcaccaa ccatgtgctg gatctgcccg
    38581 gagatcccca ccgccaggta cagttcaggt ttcagcatca ggttggagat accgacatag
    38641 cgttcgtgct ccatccattt ttcgttttcc gccaccggac gagaacaggc caactccgca
    38701 cctatcgcct tgcaaagctg ttctgccagc gcaatgttct ctttgctgcc aataccgcga
    38761 ccgacgctga ccaccagacg ggctttgtcg agatcgacgc tgttgctctg gcgcgcctgg
    38821 gttgccgtgc gggtaatcgc cacagccgga gcctgccact ccacggtgtg cgtttcgcca
    38881 gtgcgtgacg cgtctggctg agccgcatcg aacgtgccgc tgctgatggt cagtaccgca
    38941 tacggcgtgg caatgcgttc ttcgccaatc gccagaccac cgtaaaccat gtgtttcact
    39001 gtcgctttac cgtcctgtac gctgacggtg ctggcatcgt tagacaccgc cgctttaagg
    39061 cgataaccca gttttgccgc cagtaatttg ccgcgacggg tgtttggcag cagcaccagg
    39121 ccgtctgcgc cgtgctggcg aatagtgtca gccatgacac cggcgtaatc ttcgatcatc
    39181 cgatcgtccg gtttgccgtt taatttccag acatgattag cgccgagctg gattgcctgt
    39241 acgccgtcgg catcattgag gacaaaggtg ttgatttgat tagctaaagc ctgcgcaccg
    39301 ttcatcagtt ccggcagacg agaaggggta tcgctgaata cccagacttg agaaaacgtg
    39361 ttcatagcat cccctgtaat taaatgactt tgcgaagatt ttcagcaaat gcggcgatct
    39421 gttcttcgcc gtcgccttca atcacgatgc gctgacgttc gcgctgtttc ggcgcggcaa
    39481 cctgttgttc tgaccaggct gcctctgcgt taaaaccaat atccgccgcc gaccatacct
    39541 ggacgggctt tttcgccgcg ccgagaatgg ctttcatcga aggaatttgt ggggagttga
    39601 tatcagtgga aacagcaaca accgcaggca gcggaatgct taaggtttcg gtttcatctt
    39661 ccagttcgcg ctcaacggtg agggtatctg cagtcaggga gataattttg ctgacgccgt
    39721 taactgccgg aatattgagg atttcgccca ccagcagacc aacctgctgg gcataaaggt
    39781 cggaagaacc atcgccacag aggatcaaat caaagcctgc tttctgggcg gctgcagcca
    39841 gtgcgctcgc cgtttgttgc ggcagtgcct gctcgaactg gtcatcaatc accacaatca
    39901 gttcatccgg gccgcgcgat agcacatctt tacgcccttt ggcgttggtc agggctttac
    39961 cgcccacact taaggccgtc acctgcgcct ctgctgcctg ttgctttagc tggcaagccg
    40021 cttcaatagc gttcagatcg tattggctta ttttggcatc ggctttgctg aagtctaatg
    40081 aaccatcagc attattgacc gcaatatcct gttcatcagg cacgcactta tagcaagtaa
    40141 taatcttcat tgcatctcca gaaatcatga aggaaacgtc gttcatatcg gtggtgttaa
    40201 tta
//
LOCUS       DAZFXT010000032        39836 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.032, whole genome shotgun sequence.
ACCESSION   DAZFXT010000032 DAZFXT010000000
VERSION     DAZFXT010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.032"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHKW0_001764"
     CDS             complement(34..330)
                     /gene="yaaX"
                     /locus_tag="ACHKW0_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HFO7474611.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAHHDHHGGHGPGKHHR"
     gene            complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHKW0_001765"
     CDS             complement(544..1830)
                     /gene="thrC"
                     /locus_tag="ACHKW0_001765"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HFO7474612.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVESILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHKW0_001766"
     CDS             complement(1831..2763)
                     /gene="thrB"
                     /locus_tag="ACHKW0_001766"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HFO7474613.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHKW0_001767"
     CDS             complement(2765..5227)
                     /gene="thrA"
                     /locus_tag="ACHKW0_001767"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HFO7474614.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGACRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(5253..5372)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(5308..5373)
                     /gene="thrL"
                     /locus_tag="ACHKW0_001768"
     CDS             complement(5308..5373)
                     /gene="thrL"
                     /locus_tag="ACHKW0_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HFO7474615.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(5587..6273)
                     /gene="yjtD"
                     /locus_tag="ACHKW0_001769"
     CDS             complement(5587..6273)
                     /gene="yjtD"
                     /locus_tag="ACHKW0_001769"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7474616.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(6673..6813)
                     /gene="yjjY"
                     /locus_tag="ACHKW0_001770"
     CDS             complement(6673..6813)
                     /gene="yjjY"
                     /locus_tag="ACHKW0_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjjY"
                     /protein_id="HFO7474617.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            6909..7625
                     /gene="arcA"
                     /locus_tag="ACHKW0_001771"
     CDS             6909..7625
                     /gene="arcA"
                     /locus_tag="ACHKW0_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HFO7474618.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(7685..9037)
                     /gene="creD"
                     /locus_tag="ACHKW0_001772"
     CDS             complement(7685..9037)
                     /gene="creD"
                     /locus_tag="ACHKW0_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HFO7474619.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(9095..10519)
                     /gene="creC"
                     /locus_tag="ACHKW0_001773"
     CDS             complement(9095..10519)
                     /gene="creC"
                     /locus_tag="ACHKW0_001773"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HFO7474620.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(10519..11208)
                     /gene="creB"
                     /locus_tag="ACHKW0_001774"
     CDS             complement(10519..11208)
                     /gene="creB"
                     /locus_tag="ACHKW0_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HFO7474621.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(11221..11694)
                     /gene="creA"
                     /locus_tag="ACHKW0_001775"
     CDS             complement(11221..11694)
                     /gene="creA"
                     /locus_tag="ACHKW0_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HFO7474622.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            11905..12774
                     /gene="robA"
                     /locus_tag="ACHKW0_001776"
     CDS             11905..12774
                     /gene="robA"
                     /locus_tag="ACHKW0_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HFO7474623.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(12771..13418)
                     /gene="gpmB"
                     /locus_tag="ACHKW0_001777"
     CDS             complement(12771..13418)
                     /gene="gpmB"
                     /locus_tag="ACHKW0_001777"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HFO7474624.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            13470..13991
                     /gene="yjjX"
                     /locus_tag="ACHKW0_001778"
     CDS             13470..13991
                     /gene="yjjX"
                     /locus_tag="ACHKW0_001778"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HFO7474625.1"
                     /translation="MHQVVCATTNPAKIQSILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENASQRGEARSA
                     TLPLPAVILEKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(14076..14402)
                     /gene="trpR"
                     /locus_tag="ACHKW0_001779"
     CDS             complement(14076..14402)
                     /gene="trpR"
                     /locus_tag="ACHKW0_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HFO7474626.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(14492..16429)
                     /gene="sltY"
                     /locus_tag="ACHKW0_001780"
     CDS             complement(14492..16429)
                     /gene="sltY"
                     /locus_tag="ACHKW0_001780"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HFO7474627.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            16640..18307
                     /gene="ettA"
                     /locus_tag="ACHKW0_001781"
     CDS             16640..18307
                     /gene="ettA"
                     /locus_tag="ACHKW0_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HFO7474628.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(18614..19846)
                     /gene="nadR"
                     /locus_tag="ACHKW0_001782"
     CDS             complement(18614..19846)
                     /gene="nadR"
                     /locus_tag="ACHKW0_001782"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0050262 - ribosylnicotinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HFO7474629.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(19867..21249)
                     /gene="radA"
                     /locus_tag="ACHKW0_001783"
     CDS             complement(19867..21249)
                     /gene="radA"
                     /locus_tag="ACHKW0_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HFO7474630.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(21298..22266)
                     /gene="serB"
                     /locus_tag="ACHKW0_001784"
     CDS             complement(21298..22266)
                     /gene="serB"
                     /locus_tag="ACHKW0_001784"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HFO7474631.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLHS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            22372..23016
                     /gene="ytjB"
                     /locus_tag="ACHKW0_001785"
     CDS             22372..23016
                     /gene="ytjB"
                     /locus_tag="ACHKW0_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HFO7474632.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            23044..24060
                     /gene="lplA"
                     /locus_tag="ACHKW0_001786"
     CDS             23044..24060
                     /gene="lplA"
                     /locus_tag="ACHKW0_001786"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HFO7474633.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            24092..24355
                     /locus_tag="ACHKW0_001787"
                     /pseudo
     CDS             24092..24355
                     /locus_tag="ACHKW0_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(24516..25235)
                     /gene="deoD"
                     /locus_tag="ACHKW0_001788"
     CDS             complement(24516..25235)
                     /gene="deoD"
                     /locus_tag="ACHKW0_001788"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HFO7474634.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(25315..26538)
                     /gene="deoB"
                     /locus_tag="ACHKW0_001789"
     CDS             complement(25315..26538)
                     /gene="deoB"
                     /locus_tag="ACHKW0_001789"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HFO7474635.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(26590..27912)
                     /gene="deoA"
                     /locus_tag="ACHKW0_001790"
     CDS             complement(26590..27912)
                     /gene="deoA"
                     /locus_tag="ACHKW0_001790"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006213 - pyrimidine nucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HFO7474636.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQDAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(28039..28818)
                     /gene="deoC"
                     /locus_tag="ACHKW0_001791"
     CDS             complement(28039..28818)
                     /gene="deoC"
                     /locus_tag="ACHKW0_001791"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HFO7474637.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            29076..30626
                     /gene="yjjI"
                     /locus_tag="ACHKW0_001792"
     CDS             29076..30626
                     /gene="yjjI"
                     /locus_tag="ACHKW0_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HFO7474638.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDIRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            30598..31461
                     /gene="yjjW"
                     /locus_tag="ACHKW0_001793"
     CDS             30598..31461
                     /gene="yjjW"
                     /locus_tag="ACHKW0_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HFO7474639.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQITGRDNQQIKRSICLLAERGKLAELRLLVIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(31876..32658)
                     /gene="yjjV"
                     /locus_tag="ACHKW0_001794"
     CDS             complement(31876..32658)
                     /gene="yjjV"
                     /locus_tag="ACHKW0_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7474640.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEVGLDL
                     FGDDPQLERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     KPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYAVFSVRG"
     gene            complement(32655..33728)
                     /gene="yjjU"
                     /locus_tag="ACHKW0_001795"
     CDS             complement(32655..33728)
                     /gene="yjjU"
                     /locus_tag="ACHKW0_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HFO7474641.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVIDAPQANDPTFNNEDLA"
     gene            complement(33850..34011)
                     /gene="ytjA"
                     /locus_tag="ACHKW0_001796"
     CDS             complement(33850..34011)
                     /gene="ytjA"
                     /locus_tag="ACHKW0_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HFO7474642.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(34138..34743)
                     /gene="osmY"
                     /locus_tag="ACHKW0_001797"
     CDS             complement(34138..34743)
                     /gene="osmY"
                     /locus_tag="ACHKW0_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HFO7474643.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(35136..36725)
                     /gene="prfC"
                     /locus_tag="ACHKW0_001798"
     CDS             complement(35136..36725)
                     /gene="prfC"
                     /locus_tag="ACHKW0_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HFO7474644.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVQASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(36816..37493)
                     /gene="yjjG"
                     /locus_tag="ACHKW0_001799"
     CDS             complement(36816..37493)
                     /gene="yjjG"
                     /locus_tag="ACHKW0_001799"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HFO7474645.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(37508..37954)
                     /gene="rimI"
                     /locus_tag="ACHKW0_001800"
     CDS             complement(37508..37954)
                     /gene="rimI"
                     /locus_tag="ACHKW0_001800"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006474 - N-terminal protein amino acid
                     acetylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HFO7474646.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(37923..38336)
                     /gene="holD"
                     /locus_tag="ACHKW0_001801"
     CDS             complement(37923..38336)
                     /gene="holD"
                     /locus_tag="ACHKW0_001801"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HFO7474647.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            38439..39470
                     /gene="rsmC"
                     /locus_tag="ACHKW0_001802"
     CDS             38439..39470
                     /gene="rsmC"
                     /locus_tag="ACHKW0_001802"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HFO7474648.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            39738..39824
                     /locus_tag="ACHKW0_001803"
     tRNA            39738..39824
                     /locus_tag="ACHKW0_001803"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39772..39774,aa:Leu,seq:cag)
BASE COUNT         9408 a        10929 c        10115 g         9384 t
ORIGIN      
        1 ctgaatgccg gattgttacc cggcatttgt catttagcgg tgatgtttgc ctggaccatg
       61 accgccgtga tgatcatgat gagctttctt atggtggcgc ggcggtggcg gcggtccgtg
      121 tgggtgccag cgattgcctc gccattcata atgttgtttc caccagccgt ggtcgcgcca
      181 gtgaccgcca tcccagtaat aaccgcgatt atcacgatcg cctatctgta attttactga
      241 cgggactaac gtaatttccg cagcctgcgc tgccatggga gcgaccagaa ccagggaaag
      301 tgcgagtacg atagattgca tctttttcac aggttactcc ttttatcatt gcccgttatg
      361 ggtcgatatc cggagattac gcgatgagaa cggcggttgt tattctccgc aatcctaatc
      421 tctaagttac cgcatttatt gagaatttct cctttttccc tgtcgttttt ctccataatt
      481 tcttcataaa aaagccgggc tgcataaaag caaacccggc ctgattgaga taatgaatag
      541 attttactga tgattcatca tcaatttacg caacgcagca aaatcggcgg gcagattatg
      601 tgaaagcaag ggtaaatcag cacgttctgc cagctctttt ggcagatcca acgtttcacc
      661 gagaatcgat tccacgctct ctttaaattt cgccggatgc gcggtgccga ggaacaagcc
      721 atattcgcct ggattcaact ggtcacgcag cgcacgatac gctacggcag cgtgcggctc
      781 cgaggtgtag cccagttctt ttaactcacg cattgtctgt tgcgtggttt catcatccac
      841 ggctgcataa cccagctctt tcagttgcca gattttgcgg cggaacaact cttccacacg
      901 cggccagttg ttcggctgac tcacgtccat cgcgttggat aacgtcgcct gagtcgcttt
      961 gggtgaccac tgaccgtcgt gcaggaaacg tggcacggta tcgttcacgt tggtcgcagc
     1021 aataaaacgt ttcaccggca gaccgagtga cttcgccagc agacccgccg tcaaatcgcc
     1081 gaagtttccg cttggcaccg agacaaccag ctggttgcgc gcttcctgtg gcagctgcgc
     1141 aacagcttca aagtagtagc aaatctgcgc cagcaaacgg ctgatgttaa tcgagttagc
     1201 tgagtttaac cccagcgcca ctttcagttc ttcatcatca aacgcctgct tcaccagcgc
     1261 ctgacaggca tcgaaatcgc cgtcgatggc aacagtttcg atattgccgc ccaacgtaca
     1321 gaacagtttt tcttgcagtg gactgatttt gcctcgtgga tagaggataa ccactttcac
     1381 attcggtaaa ccgtagaaag catgagccac tgccgctccg gtatcaccgg aggtcgcggt
     1441 cagaatggtc actggcttat cgcccgcaat atgggtcagc atttgtgcca taaagcgacc
     1501 gccgaaatct ttaaatgcca gcgttggccc gtggaacaat tccagacaac cgacatcgct
     1561 ttcaacattg gcgaccggag ccgggaaggc aaacgccgcg cgcacgcgct cttccaggat
     1621 ttcctgcggg atttcatcac caataaacgc cgagaggatc ttcgcactgc gggtgacaaa
     1681 atccagcttc agcatctcat caatttcagt caggctgaat tccggcaggt catgcggaaa
     1741 aaacagcccc tgatttttgc ccaacccctg ggttacggct tgcgcaaagc tgacctgctc
     1801 gttgtgatct ttcagattgt agagtttcat ttagttttcc agtactcgtg cgcccgccgt
     1861 atccagccgg caaatatgaa caaaaccttc ctgattttgc aggtagttct tacccaacca
     1921 gtcggcaacg cgctgggcgg tatccggctt gtcacataga gcgaacaagg tcgggccgga
     1981 gccggagata ccgctcgcta ccgcgccgat ttccgcgacc gcctgccgcg cctgccggaa
     2041 gccaggcagt aaccgttcac ggtagggttc agcgataaca tctttcatca gcttcgcggc
     2101 aagctcaggc tgacgggaat agcaggcgtg aatgaagcca gccagatgtc gcccgtgcgc
     2161 aatgcaatcc tggcggcgat actgcgccgg taaaatagcc ctggcttctg ccgtcgagac
     2221 tttaatcccc ggatacgcca gcacccacag ccactcatca aaccctggca cttgctggct
     2281 gatgatgtcg ttttcttcga tcatcaactg cataccacca agaaaacacg gtgccacgtt
     2341 gtcgtaatga atgctgccgg agattcgtcc ttccagctcg cccatcaaag ccagcaaacg
     2401 agtgtcatta agcggcttgc cacagtgttc attcatcgcc atcagcgccg cgaccaccga
     2461 acaggcgctg gagcctaagc ccgaaccgat tggcatattc ttttccagag tcatcgccac
     2521 tggaatttgc ttgccaagct cctggcaaaa acgctcccag cactgataaa cgatattttc
     2581 ccgtggttct gacggcagct tatcggcaaa gcgtccgagg ttgttgagac tgaatgtctc
     2641 tgccgcctca accgtgacta catctccgag caatgcacca tcaacaggtg tcaccgccgc
     2701 cccgagcaca tcaaacccga cgctcatatt ggcactggaa gccggggcat aaactttaac
     2761 catgtcagac tcctaacttc catgagaggg tacgtagcag atcggcaaag acaccggcag
     2821 ctgtaacgtc attgcccgca ccatatccgc gcagcaccaa cggcagcggc tgataatagt
     2881 ggctataaaa ggccagggcg ttttcgccat ttttcacttt gaacagcgga tcattaccat
     2941 ccacttcggc aatcttcacg cggcaggcac catcttcatc aatattgcca acatagcgca
     3001 aaacttttcc ttcatcacgg gccttcgcca cgcgcgcggc aaagagatcg tcgagctgtg
     3061 acagattcgc cataaaagcg gcaacatcac cctcagcgtt aaactctgcg ggcagcacag
     3121 gttcaatttc aatatccgcc agctccagtt cacgtcccgt ttcacgagcg agaatcaata
     3181 gcttacgcgc tacatccata ccagaaagat catctcgcgg atccggttcg gtataaccca
     3241 tttcccgcgc cagagtagtc gcctcggaga aactcatgcc ttcgtctaac ttgccgaaga
     3301 tataagaaag cgaacctgaa agaatgccgg agaacttcat caattcatca ccagcattga
     3361 gcagattttg caggttctca ataaccggta atccagcccc aacgttggtg tcatagagga
     3421 atttacgccg cgatttttcc gccgcatgac gcaacagatg gtagtaatcc atcgacgagg
     3481 tgttggcctt tttgttcggc gtgacaacgt ggaaaccttc gcgcaagaag tcggcatatt
     3541 gatccgccac tgcctggctg gaggtgcagt caacaatgac cgggttcagc agatgatatt
     3601 ctttcacgag gcgaattaag cgcccgagat taaacggctc tttggcttgc gccagttctt
     3661 cctgccagtt ttccagattt aggccatgca cattggtgag cagtgccttc gagttggcaa
     3721 caccgcagac acgtaagtcg atatgtttat tcttcagcca gctttgttga cgcttcagtt
     3781 gctccagcag cgcaccgcca acgccaccga cgccaatcac aaacacttcg ataacctgat
     3841 cggtattgaa cagcatctga tgagtaacgc gcacgccagt ggtcgcatca tcgttattta
     3901 ccacgacaga gattgagcgt tcagaagatc cctgagcaat ggcgacaatg ttgatattgg
     3961 cgcgggccag cgcggcaaag aatttcgccg agatcccacg caaggtgcgc ataccatcac
     4021 ctaccaccga gataatggcc agccgttccg tcaccgccag cggctccagt aagccttctt
     4081 tcagttccag gtagaactct tcctgcattg cccgttcagc tcgcacacag tcgctttgtg
     4141 gaacgcagaa actgatactg tattcggaag atgattgcgt aatcagcacc acggaaatac
     4201 gggcgcgtga catcgcggca aagacgcgcg ccgccatgcc gaccattcct ttcatccccg
     4261 ggccggaaac gctgaacatt gccatgttat tcagattgga aatgcccttg accggtaatt
     4321 cgtcttcatc acgactggca ccaatgagcg tacctggtgc ttgaggattt ccggtatttt
     4381 taatcaggca agggatctgg aactgggcga tgggggtaat ggtgcggggg tgaagaactt
     4441 tagcgccgaa gtaggaaagc tccatcgctt cctggtagga catcgacttc aacaacctcg
     4501 catcgggcac ctgacgcggg tcgcaggtat agaccccgtc aacgtccgtc caaatctcgc
     4561 aacaatcggc gcgtaaacag gcagccagca ccgccgcgga gtagtcggaa ccgttgcgtc
     4621 caagtaccac cagttcgcct ttttcattac cggcggtgaa acctgccatc aacaccatgt
     4681 gatcagccgg aatgcggctt gccgcaatac ggcgggtgga ctcagcaata tcgacggtag
     4741 attcgaggta atgccccact gccagcagtt tttcgaccgg atcgataacg gtaacgttgt
     4801 gaccacgcgc ttctaacacg ccggccataa tggcgatcga cattttctcg ccacggcaaa
     4861 tcagcgcagc gttgatgcta tccgggcact gccccaacaa actaatgcca tgcaggacat
     4921 gttttatttg ggcaaattcc tgatcgacga aagttttcaa ttgcgccagc gggaaccccg
     4981 gctgggcggc ggcgagtccc gtcaaaagtt cggcaaaaat acgttcggca tcgctgatat
     5041 tgggtaaagc atcctggccg ctaatggttt tttcaatcat cgccaccagg tggttggtga
     5101 ttttggcggg ggcagagagg acggtggcca cctgcccctg cctggcattg ctttccagaa
     5161 tatcggcaac acgcagaaaa cgttctgcat ttgccactga tgtaccgccg aacttcaaca
     5221 ctcgcatggt tgttacctcg ttacctttgg tcgaaaaaaa agcccgcact gtcaggtgcg
     5281 ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg taatggtgat
     5341 ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg taatttttat
     5401 ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc aataaaattt
     5461 taatttactc acggcaggta accagttcag aagctgctat cagacactct ttttttaatc
     5521 cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata cttactaagg
     5581 cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca acattgccgt
     5641 gtctcgttgc tctaaaagcc ccaggcgttg ttgtaaccag tcgaccagtt ttatgtcatc
     5701 tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt gcagttgatg
     5761 ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta attgatagca
     5821 atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca tcggcacacc
     5881 agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt cgcgaccaaa
     5941 caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca ccagttctac
     6001 tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga cagtgaaatc
     6061 gacatcgtgt aacgattcaa ccagtgtcgg gaaaacttta atattatcaa taatatcacc
     6121 agatccatgt gcgacccagc gggtggctgg ctccagatgt gcctgactat cgacaatccg
     6181 cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat tttctgctct
     6241 ggcgggtgcg accagaataa tcgttatacg catattgcca ctcttcttga ccaaataacc
     6301 gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat tcttaacgta
     6361 agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc tgactgtact
     6421 aacggttgaa ttgttaaaaa atgctacata tccttctgtt tacttaggat aattttataa
     6481 aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc aactagttgg
     6541 attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat caaactgtta
     6601 acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg gtgtccggcc
     6661 cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt ttgacactgt
     6721 cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt tgatggaaag
     6781 tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt aatttgcagc
     6841 atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg gcaatttagg
     6901 tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta acacgcaaca
     6961 cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca gatggcgcgg
     7021 aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat atcaatctgc
     7081 cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat gttgcgttga
     7141 tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa atcggtgcag
     7201 atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca cgcaacctgc
     7261 tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt gaaagctaca
     7321 agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct gatggcgagc
     7381 agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa aacccaggca
     7441 aaattcagtc tcgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg aaaccacacg
     7501 accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct acgccggata
     7561 cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt gatctggaag
     7621 attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt tttatttttc
     7681 aaccttattt ccagatacgt aattgatcgt ccattgtaac ttctttactg gctttcattt
     7741 tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg ccggccagcg
     7801 ccaccaccag cacactggtt cccaacaaca gcgcgctatc ggcagagttg agcagtcccc
     7861 acatcacacc atccagcaac aacagcgcga gggtaaacaa catgctgttg cgccagcctt
     7921 tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg gcgattatcc
     7981 atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa aacattacca
     8041 atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg agcgtttcaa
     8101 aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta gtcgcccggt
     8161 ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg gggaagtttt
     8221 cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca aaccagctgc
     8281 tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc aaaaaatcac
     8341 ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta cgcccggcag
     8401 gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc ttcaggttct
     8461 gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct tgctctaacc
     8521 cggtaccggg ttcaatggtc agtgccgttc cgttaacttc aggcgctttc accacaccaa
     8581 taccacgcgc atccccgacg ctaatcacaa taaagggctt gcctaagatg atatttggcg
     8641 cattgagctc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa tcactgtgcc
     8701 agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg ccatcaacca
     8761 tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg gttttatcct
     8821 cttcctgcac cgtataaagc tcggtcacag gaatagcgat aagcggccca acgagttttt
     8881 gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa tcggcacgtt
     8941 cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact gcaccaaaca
     9001 gggtggtcat tttccagaac aggggggatt tcaacatagt cttctccttt gcaatggcag
     9061 caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg tgaagtcgaa
     9121 gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg ttaaacaaac
     9181 gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca tttgcacgcg
     9241 gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata ccactaccgg
     9301 tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc gttatacgac
     9361 cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc tgatccagta
     9421 acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg atgttttttt
     9481 ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc acatcaacag
     9541 cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac gtttccacca
     9601 gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc actacttcag
     9661 gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt gggcttttta
     9721 gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc agcttcacgc
     9781 gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga tcgggaagag
     9841 gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca atggagcggt
     9901 tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa atggcgctgg
     9961 cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg ttcggtttgc
    10021 ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca acatacatca
    10081 ccgaactttc gggatcggca ggattttgca acgtgctgcg cgcaccatac tgaccacgca
    10141 acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta tttgccgaat
    10201 cgaacaatac tttaccctgc gaatcggtca tatagacatg atattcattg cgcactttgt
    10261 taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac gcctgcgcca
    10321 gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc gccagcaacg
    10381 ttgcggtgtc gatcaacgtc ccctccgttg ctcttcgcac gcccggttta acttctttga
    10441 caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag cccagcagca
    10501 accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc gatgagtatt
    10561 aatcggtgaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt taatgtgggt
    10621 atcaacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca tcagttgctg
    10681 gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa actcataccg
    10741 cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat tcaattcaaa
    10801 atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac gcagtaaggt
    10861 gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt cgtcagcgcc
    10921 aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga acagtacagg
    10981 taacgccgga tggagcgcca gtaactggcg gcacaattca aagccactga tatcaggcaa
    11041 accaacgtca agaatcatga cgtcgggaac ctgctggcga gctttatcca gcaccggcaa
    11101 acctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga ccagcgtgtc
    11161 ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa atcgcctctg
    11221 ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg aaccttctac
    11281 aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat aaaagcgcac
    11341 gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct cgccctgagc
    11401 tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac aagaaatggc
    11461 cgcatcggag gtatcttctg ccaggcccaa tcccccttta ataccaccgg ttttcgcccg
    11521 gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca caacaatttt
    11581 gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt ctgcatgagc
    11641 caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt tcatattgtt
    11701 accattttta tctattacac gagcaattag ttcgtcacgg taaataacat attgattaag
    11761 aacaaaatct caatactttt atttccgtca agccctaaaa catactctac taagaaaaaa
    11821 acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt caggtgctcg
    11881 ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga ccttttaatc
    11941 tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc gaaagcaggt
    12001 tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc tattggcgcg
    12061 tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac tgcgcgtccg
    12121 attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac ccgcgcattc
    12181 aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg gagcgccttt
    12241 ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa atttgtcacc
    12301 ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct ggagcaaatc
    12361 tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg caacgcgccg
    12421 accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga taaagacgac
    12481 gaacaagagg tattctatac cactgcgtta gcccaggatc aggcagatgg ctatgtactg
    12541 acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta tgaaggtctg
    12601 ggaaccggcg tgcaggagtt tatcctgaca gtatacggaa cgtgcatgcc aatgctcaat
    12661 ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga tgccaaagcg
    12721 ggagatcgcc caattaatct gcgctgtgaa ctgctgattc cgatccgtcg ttaacgctgc
    12781 aactcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc aacaacccag
    12841 ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca gttacgcaga
    12901 cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag acatcccagt
    12961 gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg gcaagattcc
    13021 agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg aatacgcccg
    13081 tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt cagagaatcg
    13141 atatgtcttt tttccagcac acccatgttt aattcacgca ggcgagaatc aaagatgatg
    13201 tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc taaatcgcta
    13261 ctgatgatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat cgcctgttgc
    13321 tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc ggcgttccac
    13381 tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg tatactgctg
    13441 taacgttaat tttctgagta atgctgatta tgcaccaagt tgtctgtgcg accaccaatc
    13501 ccgctaaaat tcagtccatt ctgcaggcat ttcacgagat cttcggcgaa ggatcctgcc
    13561 atattgcatc cgttgccgtc gagagcggtg taccggaaca accctttggc agtgaggaaa
    13621 cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca gaggctgatt
    13681 tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg gtggtgattg
    13741 aaaacgccag ccagcgcggc gaggcgcgtt cggctacttt gccgttgcct gcggtgattc
    13801 tggaaaaagt gcgtgaaggt gaggcacttg gtcccgtgat gtcgcgttat accggtattg
    13861 atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa ctcactcgcg
    13921 ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcataat gcggtgtatc
    13981 aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc acgatgcctg
    14041 atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag caacacctct
    14101 tccagccact ggcgcagctc aacgggcgcg gctttcaggc tgttagatcc acgcgtaatc
    14161 gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct ggctcatttc gccgcgcaac
    14221 agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg cgtcagcatc
    14281 aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag gtcgacaaaa
    14341 cgtaaccact cttggtgacg ctgttctgcc atcgctgctg aatagggtga ttgttgggcc
    14401 ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg tactcgctaa
    14461 agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat tccgtggcgc
    14521 tcatcaacgt cggtttatcc cccatgaaat agcggtagta agcgtcataa gccagcacgt
    14581 tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc acagcgtcga
    14641 tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg ttataagctg
    14701 ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg taactggtgc
    14761 caatgttgat gttcgtttcc ggatccagca attgcccagg gctgctataa ccaggaatag
    14821 agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg ccgctggccc
    14881 ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc gccatcgcat
    14941 agctttgcgg gatctcctta ccgctggtgt agcgtttgaa aagatcgttg taagccagcg
    15001 ggaatcgctc ttccagatga tcccacagct tcccggcgat cgttgcctga acgctaagat
    15061 cccaccattg gttgttgaaa gcataccgcg ccagttgagc ctgctctgtt tttgacttgc
    15121 tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag tacatcaact
    15181 cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc tgcggcgctt
    15241 tatcaatctt cagctcatac tcttcgccga tgcgttgtgc agcaaccatc gggtagaaac
    15301 cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt ccgcgttcca
    15361 gcaataaatc cgcctgccag taacgccatt catctttctc ttttgcttcc atcggcagac
    15421 gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagcgccatt cgcacacggc
    15481 gttcaatcaa cgacgtggat tgcgagcgca taatggcgtc atcgcgccat ttcgcctgct
    15541 cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc tcctgaatct
    15601 gatcttcatt aagctgctgc gcctgggcaa gcgacgggat catcagccgt gcattttcag
    15661 catcctgccg cgccacactg gcaaacgcca ccgccgccat ttgacgggta aaatcggtcg
    15721 cgccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt gaaatgattg
    15781 ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt accaggccag
    15841 tgttacccgc tttcatcgcc aggcggatac gctctaaata cgccagcgga tcttgtttac
    15901 ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc ttgccggtta
    15961 gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc catttcgcat
    16021 agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag gctaacaagc
    16081 cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc gtgcgagcgg
    16141 gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt tgattcatca
    16201 ggtcatcggt gatctggcgg tattccaggt agggataaag cggataatcc ttcagtccag
    16261 gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg atttgcgcgt
    16321 aacgactacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc gtcagcagac
    16381 agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca agcgcatcct
    16441 ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc taaccagtca
    16501 ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt gatcgaccaa
    16561 ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca tcacgataaa
    16621 catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg tcggcaaagt
    16681 tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc ctggggcaaa
    16741 aattggtgtc ctgggtctga acggcgcggg taagtccacc ctgctgcgca ttatggcggg
    16801 cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga ttggttatct
    16861 gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtctattg aagaagcggt
    16921 ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt acgccgatcc
    16981 ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga ttattcaggc
    17041 tcacgacggt cataatctga acgtacagct ggagcgtgcg gcggatgcgc tacgtctgcc
    17101 ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg tggcgttgtg
    17161 ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca accacctgga
    17221 tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgaaggca ccgtggtggc
    17281 gattactcac gaccgttact tcctcgataa cgttgcaggc tggatcctcg aactggaccg
    17341 cggtgaaggt attccgtggg aaggtaacta ctcctcctgg ctggagcaga aagatcagcg
    17401 tctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga aagagctgga
    17461 gtgggtacgt cagggaacta aaggccgtca gtcgaaaggt aaagcacgtc tggcacgctt
    17521 tgaagagctg aacagcaccg aataccagaa acgtaacgaa accaacgaac tgtttattcc
    17581 acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta aatcctacgg
    17641 cgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcga tcgtcgggat
    17701 catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctccg gtcaggaaca
    17761 gccggacagc ggcaccatca ctttgggtga aacggtgaaa ctggcatcgg ttgatcagtt
    17821 ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg ggctggatat
    17881 tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct ttaactttaa
    17941 aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg gtcgtctgca
    18001 tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac caaccaacga
    18061 cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc cgggctgtgc
    18121 gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca tcctggacta
    18181 ccaggatgaa ggtaaagttg agttcttcga aggtaacttt accgaatacg aagagtacaa
    18241 aaaacgcacg ctgggcgcag acgcgctgga gccgaagcgt atcaagtaca agcgtattgc
    18301 gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta caaaccaaga
    18361 aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc gcatcaggca
    18421 ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac agccttagta
    18481 tccgcaaatt cgtagcgggt cacccgtgct gattctttta aaccttgttt aatctcgaca
    18541 gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc gcctttgagc
    18601 cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc acgcagcgca
    18661 ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg ttctcttcca
    18721 gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg aggctgcgta
    18781 aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg aaacggtatt
    18841 catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg cagaacgcct
    18901 gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc actgcaaaat
    18961 caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc aatgcgatct
    19021 catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca ctggtggtat
    19081 tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg ccaccaagga
    19141 tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc cagtagcgga
    19201 acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc ggatcgacca
    19261 gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct tccgaggtgt
    19321 agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg ttgctccaca
    19381 catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga atgcgaatat
    19441 ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc gtgggctgct
    19501 gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa cccataatga
    19561 tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt ccggtatgca
    19621 gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga aattcaagcc
    19681 ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt ttggcattca
    19741 gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc tgtagcgtgc
    19801 agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct ccctccgtat
    19861 ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa cgccaaaaat
    19921 ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg cccggcgaaa
    19981 accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc ggatctcccc
    20041 tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt cacgcaggct
    20101 ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct tcacgccgcc
    20161 gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac ggtgcaagac
    20221 agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc gcggattcgc
    20281 catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg ttccttccca
    20341 caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga aaattgccga
    20401 agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca gctcattcac
    20461 cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg catcaccatc
    20521 cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca gcgagccatc
    20581 tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga agcgcgtcag
    20641 ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta catccgccat
    20701 atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt cggcaatcag
    20761 gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg gcaggccaag
    20821 gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat acagcgtttt
    20881 catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc ccgcacctgg
    20941 gttaccgcca atcagaatgg cgcttcctgg caccacgccg ccgcctagta cgcggtcgaa
    21001 ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg agagtttctg
    21061 gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac gcgccaccgt
    21121 tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg cactgcactg
    21181 cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc gttttggagc
    21241 ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg cgagcaatta
    21301 cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag cgtgacggat
    21361 ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc ccagccctgc
    21421 tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct gcgccagcgg
    21481 gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg cgtctacgat
    21541 gtcgccgatc acattgccgg taaatttacc gtccatgatc tccagttcat tggctaccac
    21601 ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc cgccggaggc
    21661 aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta agcctggcat
    21721 cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt tcagcgtcgc
    21781 cacacggctg tgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc gttcggttac
    21841 ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac attcaatctg
    21901 gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt gcgggatttt
    21961 ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc gtgcggtgag
    22021 tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa caatcaccat
    22081 cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc cacgaccata
    22141 cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat caccacttaa
    22201 tgaaagaggc agaccaggcc ataaagagac atcttcaggc aggtcgcacc aggtaatgtt
    22261 aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg aggctacctt
    22321 gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa gatggctcgc
    22381 acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct tgccttgtta
    22441 gtggcgctga tgcagggagc gtcatggttt agtcaaaacc accagcgaca acgtaatcca
    22501 cagctggaag aactggcccg caccctggcg cgtcaggtaa cgctgaacgt tgcaccgctg
    22561 atgcgtaccg actcaccgga tgaaaaacgc attcaggcga tcctcgatca gttaacggat
    22621 gaaagccgta tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat cgcacgttct
    22681 ggcgaaagcg tcgaagtgcg cgaccggctg gcgctcgacg gtaaaaaagc gggcggctat
    22741 tttaaccagc agattgtcga accaattgcc gggaaaaacg gaccgctcgg ctatctgcgc
    22801 ctgacgctcg acacccatac gctcgccacc gaagcccaac aggtggataa caccactaac
    22861 attttacgcc tgatgttgct gctttcactg gcaatcggcg tagtgctgac ccgcacgctg
    22921 ctacagggta aacgtacccg ctggcagcaa tcgcccttcc tgttaaccgc cagcaaaccg
    22981 gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag aaaggaaatc
    23041 gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt taacctggcg
    23101 gtggaggagt gtatttttcg ccagatgccc gccacgcagc gcgttctgtt tctctggcgc
    23161 aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg taatacccgg
    23221 cggatggaag aagataacgt ccgcctggca cggcgcagta gcggtggcgg cgcggtgttc
    23281 cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta cgataaaacc
    23341 atctccacgt cgattgtgct caatgcgctg aacgcgcttg gcgtcagcgc cgaagcgtcc
    23401 gggcgtaacg atctggtggt gaaaactgcc gaaggtgacc gcaaagtctc aggatcggcc
    23461 tatcgcgaaa ccaaagatcg tggcttccac cacggcacct tgctgctcaa tgccgacctt
    23521 agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa aggcattacc
    23581 tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac ccatgagcag
    23641 gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt agaagcggaa
    23701 atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt tgcccgtcag
    23761 agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct ggatgaacgc
    23821 tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat cacccgcgcc
    23881 caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg gcgactgcaa
    23941 ggcggcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt ggttgacttc
    24001 ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc ggtaaggtaa
    24061 ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc aggagcggga
    24121 gattatcaat ataatgttaa gccagcgtat ttcctggcta gccgccgtgc gtatcaggca
    24181 taaattatcg cttgctgaag taagtaaaat gttaggtatc tcgattaatt ctctaaaata
    24241 gatcgaaaag acggaacggt ttagctcaaa catcaaaagc aaaatggcag aaatttatgg
    24301 ttgtccacca gagttgctga tatgcccttc ctggatgacg gctgaacata aatgaataga
    24361 ttcgtgaata gatacttgaa tagattttat tttctatcca tatgaaaaat aatagtaaat
    24421 ctataaatca gttgctattg atgaatagat actaaaaagc cggagcagtc tcccactccg
    24481 gcttcacacg acaatcgcct ttcagcgaaa cacaattact ctttatcgcc cagcagaacg
    24541 gattccagtg cgattttgat catgtcgttg aaggtggtct gacgctcagc ggcagtggtc
    24601 tgctcgtgag tgcggatgtg gtcagacacg gtacagatgg tcagggcttt cgcgccaaat
    24661 tctgcagcga cgccgtagat accagccgct tccatttcca cgccgaggat gccgtatttt
    24721 tccatcacgt cgaacatttc gccgtccgga gagtagaaca ggtcagcgga gaacaggtta
    24781 ccaacgcgag catcaatgcc cagtgcttta gccgcgtcta ccgcgttacg caccatgtca
    24841 aagtcagcga tagcggcaaa gtcatgatct ttaaaacgga tgcggttaac tttggaatcg
    24901 gtgcaggcac ccataccgat aacgacgtcg cgcagtttta cgtgcggcag aactgcgcca
    24961 caggaaccca cgcggataat tttcttcacg ccgaaatcgg tgatcagttc tttggtgtag
    25021 atggagcagg acgggatacc cataccgtga cccattacgg aaattttgcg gcctttataa
    25081 gtaccggtga agcccagcat accgcgaacg ttgttcactt cacgggcatc ttcaaggaaa
    25141 gtttcagcaa tatactttgc acgcagcggg tcgcctggca tcaaaactac gtcagcgaaa
    25201 tcgcccattt ctgcattaat gtgtggggta gccattgttt tatccttatc tacatgtttt
    25261 caccctctcc ctgagggaga gggccggggt gagggcatca agccgcaccc attatcagaa
    25321 catggctttg ccatattcca tatcagaagt accaaaatat tttgccagag tctggccgat
    25381 atccgcgaag gtttcacggt gacccagtga gcccggtttt actttcgggc cgtaaaccag
    25441 taccggaatg tgttcacgcg tgtgatcagt accggtccag gtcggatcac aaccgtggtc
    25501 agcagtgagg atcaggatgt cgtcatcgcg cagcagagac atcagctccg gcagacggcg
    25561 gtcgaacagt tccagacccg ccgcataacc tgcgacgtcg cgacggtggc cccaggaaga
    25621 gtcgaagtca acgaagttgg tgaagacgat ggtgttatca cccgcttctt tcatctcttt
    25681 gatggtggcg tcaaacagcg cgtccaggcc agttgctttc actttcttgg tgataccgca
    25741 gttggcgtag atgtctgcaa ttttaccgac agaaaccacc tgaccgtgtt tttcatcaac
    25801 cagtttctgc agcacggtcg gtgctggcgg ctcaacagcc aggtcgtgac ggttaccggt
    25861 acgctggaag ttaccggctt tgtcgccaat aaacggacga gcgataacac gaccgatgtt
    25921 gtagccgccg ttggtcagct cttcacgggc gatttcgcac agttcgtaga gtttatccaa
    25981 gccaaaggtt tcttcgtggc aggcaatctg gaacacggag tcagcggagg tatagaaaat
    26041 cggcttgccg gttttcatgt gctcttcgcc cagctgatcc agaatgaccg taccggaaga
    26101 gtggcagtta ccgagataac caggcaggtt agcgcgttca accagtttat ccagcagctc
    26161 ttgcgggaag ctgttttcgt gatcggagaa gtatccccac tcaaacagaa ccgggacacc
    26221 ggcgatttcc cagtgaccag acggggtatc tttaccggat gacatttcgt gcgcccatgc
    26281 gtacgcgccg ataacttcag cgttgccgtc cattcctgcc ggaatgaaac cggtagaacc
    26341 ttcatgtgct ttcgccagcc ccagacgggt cagatttggc agattgagcg ggcctttacg
    26401 accgttatca gcttcgcctt tggcacaggc ttctgcgata tgacccaggg tgtcagcccc
    26461 gacgtcacca aagcgttctg catcttctgt agcgccgatg ccgaatgagt ccagcaccat
    26521 aataaatgca cgtttcatat gttctccgta ctcagtgctt caaatttaaa tgatcagatc
    26581 agtataccgt tattcgctga tacggcgata gacagttggt gtgctttccg gtgctttatc
    26641 ggcaagttta attgccgctt tcaccgcttt cgccgcgtcc tgccagctgt tttcgtcttt
    26701 cgcgtggata acagccagcg gacgctgacc gtctacctgg tcgcccaaac gcgccatatc
    26761 agtaaagccg acgctgtaat caatggtgtc agatgcctga cgacgaccgc cgcccattgc
    26821 aaccactgcc atccccagcg cgcgggtatc catttcactg acaaaacctt cggtatcagc
    26881 atagactgct ttcgtcagca tcgctgtcgg cagatacttc gcgtagttct caacgaagtc
    26941 ggttgggcct ttttgtgccg ctaccatacg accaaagact tctgccgctt taccgttgtc
    27001 cagcactgct tgcaatttcg cgcgcgcttc ggcgtcatct ttcgccagtt tgccggagat
    27061 aagcatctcc acgcacagtg ccatcgtgac atcaaacaga cgcgggttac gatattcacc
    27121 cgtcaggaac tgcaccgctt cacgaacttc aaccgcgtta cctgcactgg aggccagtac
    27181 ctgattcatg tcggtgagca gcgcggtggt gcgcacgcca gcgccgttag ccacgccaac
    27241 aatcgcttcg gcaagggctt cagagagttc gtaggtcggc ataaacgcgc cgctacccac
    27301 tttcacgtcc atcaccagcg catccagacc ttccgccagt ttcttcgcca ggatcgaggc
    27361 agtgatcagc gggatggagt ccacggttgc ggtaatatcg cgggtcgcgt agaaacgttt
    27421 atctgccgga gccaatgagc tggtctggcc gataatcgcc acgccgacgt ctttaataat
    27481 ttcgcggaaa cggttgtcat ccgggaaaat gtcgaagcca gggatggatt ccagtttgtc
    27541 gagcgtaccg ccagtatgac cgaggccgcg accagagatc atcggaatat agccgccgca
    27601 ggctgcgacc atcggcccca acatcagcga agtcacatcg ccgacgccgc cggtcgagtg
    27661 tttatcaaca atcgggccat tcagatgcag gcttttccag tcgagaacgg ttcctgaatc
    27721 tcgcatcgcc atggtcagcg agacacgctc aggcattgtc atatcgtgga agaaaatggt
    27781 catcgcgagg gcggcaatct gcccttcgga gatagtgttg tcgcgaatac cgttgataaa
    27841 gaaacgaatt tcttcatcgc tcagcgcatg accatcacgt tttttacgaa taatttcttg
    27901 tgcgagaaac aaggtaacct cccgaggaaa agagtatgag gcggcttttg ccggatgcgt
    27961 ctacgcctta tccggcctac gggtagatac gtctcgtagg cctgataagc gcagcgcatc
    28021 aggcgtaaag catcttactt agtagctgct ggcgctctta ccgtcgccgt ggcccagagc
    28081 cttcagcagg cttgccagca ggctggaagc accaaagcgg tagtggcgcg catcggccca
    28141 gtcagcgccg aacagttcat ctgcgatggc gagatatttc tgcgcatctt ccgcagtacg
    28201 cacgccgccc gccggtttga aaccaacggt tttttctacg cccatatcac gaatcacttc
    28261 catcatgatg cgcgcgcttt ccggcgtcgc gttcactgcc actttaccgg tagaggtttt
    28321 gatgaagtca gcacccgctt tgatggagat ttcagacgct ttacggatca gcgcttcgtc
    28381 tttcagttcg ccggtttcga tgatcacttt cagcagtaca ttcgctgccg cgcaagcctc
    28441 tttacaggct ttcaccaggt caaaaccaac ctgctcgtta cccgccatca gcgcacggta
    28501 cgggaacacc acgtcaactt catcggcacc gtaggcgatt gccgcacggg tttctgccag
    28561 cgcgatttcg atgtcgtcgt taccgtgtgg gaagttagtt accgtagcaa tacggatttc
    28621 cggggtgccc tgctctttca gcgttttgcg agcaatcggg ataaagcgag gatagataca
    28681 gatagcggcg gtattgccga ccggagtttt ggcctgatga cacagagcaa ttactttctc
    28741 gtcggtgtcg tcgtcattca gggtggtcag gtccatcaat ttcagtgcac gcaggctgct
    28801 tgctttcaga tcagtcattt cattctcctg gcttgtcgcc aataaaattc accttgcgag
    28861 tttgttagta ttctaacatc tactccgcaa cacacttcga tatacatcac aattaaggaa
    28921 atctacttac aagtttgcat cactgtaatg cgatctggtt caaataattc actttcaaat
    28981 gaatgcgtca gtggtggcaa acgcatcagg atcaaagtga acatcacgaa acttcttaca
    29041 atggcgcatc accttcggca taaaaggaaa agaagatgcc cacttctcat gaaaatgcac
    29101 tgcaacaacg ttgccagcaa attgtcacca gcccagtgct tagcccggag cagaagcgcc
    29161 attttctggc tctggaagca gaaaacaatc tgccttatcc gcagcttcct gccgaagccc
    29221 gccgcgcgct ggatgagggt gtaatctgcg atatgtttga aggtcatgcg ccgtacaaac
    29281 cgcgctatgt cttacccgat tacgcccgtt ttctggcgaa cggttccgaa tggctggagc
    29341 tggaaggcgc gaaagatctt gatgacgcac tctctctgct gaccattctt taccaccacg
    29401 taccgtcggt cacatcgatg ccggtctacc tggggcaact ggatgcgttg ttgcaaccgt
    29461 atgttagaat tctaacacaa gacgagatcg atattcgaat aaaacgtttc tggcgttacc
    29521 tcgacagaac cctgccagac gcctttatgc acgccaatat cggcccgtct gattcgccca
    29581 ttacccgcgc gatcttacgt gcagacgcag agttgaagca ggtttcaccg aacctgacct
    29641 ttatctacga tcctgaaatc acccctgatg acctgctgct ggaagtggcg aagaacatct
    29701 gtgaatgtag caaaccgcac atcgccaacg gtccggtgca tgataaaatt ttcacaaaag
    29761 ggggctacgg gattgtgagc tgttacaact cactgccgct ggcgggtggt ggcagcacgc
    29821 tggtacgcct taacctgaaa gccattgccg agcgcagcga atcgctggat gacttcttta
    29881 cgcgcactct accgcactac tgccagcagc agatcgccat catcgatgcg cggtgtgaat
    29941 tcctctatca acaatcacac ttctttgaga atagcttcct ggtgaaagaa gggctgatta
    30001 accctgaacg ttttgtgcca atgtttggca tgtatgggct ggcggaagcg gttaacttgc
    30061 tgtgtgagaa agaagggatt gccgcacgtt acggtaaaga agccgccgca aatgaagtgg
    30121 gttatcgcat cagcgcgcaa ctggcggagt ttgtcgccaa tacccccgtg aaatatggct
    30181 ggcaaaaacg cgccatgtta cacgcacagt cggggatcag ttccgatatc ggcaccacgc
    30241 cgggcgcgcg tttgccgtat ggcgatgagc cagatccgat cacccatctg caaactgtcg
    30301 ccccgcatca tgcttattat tattccggca tcagcgacat tctgaccctc gacgaaacca
    30361 tcaaacgtaa cccgcaggca ctggtacagc tttgcctcgg tgcctttaaa gccggaatgc
    30421 gtgaatttac cgccaatgtc agcggtaacg atctggttcg cgttaccggt tatatggtgc
    30481 gtttgtcgga tttagaaaaa tatcgcgccg aaggttcacg caccaacacc acctggctgg
    30541 gcgaagaagc cgcacgcaac actcgtattc tggaacgcca gccgcgcgtg ataagccatg
    30601 aacagcagat gcgctttagt cagtaagatt atccccttct cctgcgttga cgggccaggc
    30661 agtcgtctgg ctctgttttt gcagggctgc aatctgcgct gcaaaaactg tcacaatccg
    30721 tggacgatgg gacgttgcaa tgactgtggg gagtgcgtgc cacagtgtcc gcatcaggcg
    30781 ttgcagattg ttgacggcaa agtggtgtgg aacgctgtgg tctgcgagca atgtgatacc
    30841 tgcctgaaga ggtgtccgca acatgccacg ccgatggcgc aatccatgag cgtggacgaa
    30901 gtgcttagcc atgtccgcaa agcggtgctg tttatcgaag ggataacggt gagtggcggc
    30961 gaagccacga cccagctgcc gtttgtggtg gcgctgttta ctgctatcaa aaatgatccg
    31021 caactgcgcc atctcacctg tctggtggac agtaacggta tgttgagcga gaccggctgg
    31081 gaaaaattgc tcccggtgtg cgacggcgca atgctcgatc tcaaagcatg gggtagcgaa
    31141 tgtcatcaac aaataaccgg acgcgataat cagcagatta agcgcagcat ctgtttgctg
    31201 gcagagcgcg gtaagctggc ggaactgcgt ttgctggtga tccctggcca ggtggattat
    31261 ttgcaacaca tcgaagaact ggcggcgttt atcaagggac tcggcgatgt tccggtacgc
    31321 ctgaacgcgt ttcatgccca cggcgtgtat ggcgaggcgc aaagctgggc gagcgccacg
    31381 ccggaagacg ttgagccgtt ggctgatgcg ttaaaggtgc gcggggtgag ccggttgata
    31441 tttccggcgc tctatttgtg acatgccgga tacggcctac ggttcggcac agacttgtag
    31501 gcatgataag acgcgacaag cgtcgcatca ggcattatgc gccgactgcc ggatgcggcg
    31561 tgaacgcctt atccggccta cggttcggca cagacttgta ggcatgataa gacgcgtcag
    31621 cgtcgcatca ggcattgtgc gccaactgcc ggatgcggcg tgaacgcctt atccggccta
    31681 cggttcggca caaacctgta ggcatgataa gacgcgtcaa gcgtcgcatc aggcattgtg
    31741 cgccaactgc cggatgcggc gtgaacgcct tatccggcct acggttcggc acaaacctgt
    31801 aggcatgata agacgcgaca agcgtcgcat caggcattat gcgccgactg ccggatgctg
    31861 cctacgaagg gtaaactacc cacgaacgct aaacaccgca tacgtattat taagcaacac
    31921 ttcggcaatc tcatcttctg gttccggacg taactcgcaa agcacgtcaa acacccgcac
    31981 agcctgttcc gggcggttag gcttcccctg aaagccgttg agcggcatat ccggcgcgtc
    32041 ggtttccagc aacaacgatg ccagcggtaa ttttgcgatg acatcgcggg ttttactggc
    32101 gcgtggatag gtgatagtac cgcctacgcc gattttgtag cccagctgta caaaccgctc
    32161 ggcctgttgc aggctgccgg aaaaaccgtg aaccacgcca gtgcgcgata aatcgtggcg
    32221 tttaagatgc atcgccagtt tgtcgtgtgt gcgccgtgaa tgcaggatca ccggcagatc
    32281 gtagcgtttc gccagtttca gctgttcgtc gagtaaccac tgctgcctct caagttgcgg
    32341 atcgtcgccg aagagatcca gaccgacctc ccccactgcc accaccttcg ccggacgcct
    32401 ttccagcgcc tgctgtagct gctcaagaga cacatcgcta tgtttttcca acatacctgg
    32461 atgcaagccc aatgcggcat acagcggttg atatttttcc gctaacgcct gcacacgggc
    32521 aaaattttcc gcctctgtcg ccggaacaat gatcttgcct acgcccgctt gtgccgcgcg
    32581 ttgcaggctg gcctcttcat cgccactaaa cggcgggaaa tcaaaatggc agtgggtgtc
    32641 gataaaacgg caaatcaagc cagatcctca ttgttaaatg tcgggtcgtt cgcctgcgga
    32701 gcgtcaatca cttcggcaac cagcgtatcg ttggcgactg gcgcaggcgg aataacgatc
    32761 gattccggcg tcaccactgg caccagatgg cgggtaagcg gcgctttttc agttaatagc
    32821 ttgccaaccg tggcgaggaa ataacgaccg cataaacgcc caagtttata gtcttcacgc
    32881 agcgccggaa tccgactgcc aagcgcgata ctatgtaatg gcttcggcgg ataaatttcg
    32941 aatatccgca gcttgcccgg tggtttctca ataaattgct gaatgtcacg atagctggtt
    33001 tcatgatgct gcaccagatt gaccagcggc tgcaggctac tgtcacccag ccagcgttcc
    33061 atgcgtttga accactgcgg cgtgtagtac atttgtgacg gcacagtgcg aatgacgacc
    33121 aacgttttag cgccctgcct tgccgcctct ttaaccggaa tcgcatcact gatcccgcca
    33181 tccaggtagt taatgccttc cagcgacact ccgctacgat aaaagccagg tatcgcactg
    33241 gaggcgcgaa tcacatccag ccagttttgt ttggttggta aaaagtaatt aggcgcgtag
    33301 tcatcctgac gacaggcgca catataaaac gatttgccgc tgtcaaacaa ccgcgcggcg
    33361 gtatccattt gcaacggcat ctggcttgcg gtggcctcca ccagccagtc gagatcgata
    33421 agatttcctc cacggacaaa gcgcaatgga tcgaaaaatt cgcgttttgt ggtatagcgc
    33481 atgatgactt tgcgcgcgta accgggctga ttgcagataa acgccgagag gttctgcgcc
    33541 ccggcagatg tgccgagata aagatcgaaa ggattaaact gcgcgcgcat aaactcatcc
    33601 agcacgccag ccgtgaaaat tccacgctgt ccgccgcctt cgcacaccag agctattcgt
    33661 ccaggctgga acggccttag cgacaacggc gcaatattac caagcgttac aggtattcgc
    33721 tgccccacct ctgccttcct gtttttgttg tgaaagaatg acaaagtaac gcaatttatt
    33781 attcgctaaa accgcaaaag ccagtccgct ggactggctt tggataaaat tttcttaatg
    33841 gtctgaaatc tagggtcgtt ttcggcccat gaacaaactc accaggaaca gaataatccc
    33901 gacgacaaag acaattttag ctgcgcctgc agcggtaccg gccagaccac caaacccaag
    33961 tgcggcggcg attaacgcga taaccagaaa tatgatgccc caacgaaaca taagcgtctc
    34021 ctttaccata gttaatgtca ccgcttgata tgagcgtgaa aatcactcaa tgcgatattt
    34081 ttagtgtggt gcacatcgcg cctcccgaca aagttcggga ggacgaatta cgacgaatta
    34141 cttagttttc agatcatttt taacgctttt cacaccatct accgctttgg cgatactttc
    34201 agcacggtcg ctctgtgcct gggaatcgac ggtaccggag agctgaacca cgccgtcggt
    34261 ggtttcaact ttcacatgac gggaagggac gatatcgtcc gccagcagtt tggctttgat
    34321 ttcactggtg gtggcggtgt cacccgcgta gcccttcaca gagccttctt tagcgtcgcg
    34381 aacgtgcagt ttgtcgctga cagaggtcac cccttcaacg cctttcgcca ctttcactgc
    34441 ctcttcggcc tgagcctggc tttcaacgaa accgctcagg gtcacgactt tttgatcggt
    34501 tttgacagag atatcggtgc tcttgatgtt gtcatgatcc accagcgccg ccttcacttt
    34561 cgcggtgatg gcgctgtcat ccatgaaatt accgacttta ttcatagagc tatcgacttt
    34621 ttgccctgcg ctttcattgg tagtctgtgc gttgttttcc gcgtaggcag agccggtcgc
    34681 gacggcagag gtcaacatta cagccagcag agttttcgaa atcttcagtc ttgtcatagt
    34741 catcgattta ttcctgtatg tttgctcgta atttgagcct ggcaacacga ggttgcattg
    34801 ctgaataggg agagacttca ccctctacag aagtcaatgg tcgccatcac aaaagcgatg
    34861 ggtgatgaat aacgaccatt acagcctctg aatcagttat taatatcggt agaatgacaa
    34921 tcgacggctt tagatactga tatctacgca ttgaacggta tttaacgccg tcagaaatgt
    34981 cattactttg ttaaatatag atcacaattt tgaaaccgct cgggatatca gcgaaaacat
    35041 aagcaaaagt gaatgtttta agaacattcc gtaagcggct aataaggaag ggaaattgac
    35101 agggcgcagc ggctaccgcg ccctggaggc aagaattaat gctcgcgggt ctggtggaac
    35161 tgaacgtcgg gataacgttc ctgcgccagg cgcaggttga ccatgctggt agcgatgtaa
    35221 gcgaggttat cgccgccatc aagcgccagt tggctttcgt tcttacgctt gaactcttcg
    35281 aatttcttcg cgtccgcaca ttctacccag cgggcagtgg caacgttgac tgattcatat
    35341 actgcttcaa cgttgtattc gctcttcagg cgcgatacca ccacatcaaa ctgcagcaca
    35401 ccaacagcac caacgatcaa atcgttgttg gagatcggac ggaacacctg caccgcgccc
    35461 tcttcggaaa gctgtaccag ccctttgagc agctgttttt gcttcagcgg atctttcagg
    35521 cggatacgac ggaacagttc tggtgcgaag ttcggaatac cggtgaactt catcatctca
    35581 ccctgggtaa aggtgtcgcc gatctgaata gtgccgtggt tgtgcaggcc gaggatatcg
    35641 cccggatacg cttcttcaac gtgcgaacgg tcgcccgcca taaaggtcag cgcgtcggag
    35701 ataaccacat ctttcgcagt acgcacctgg cgcagcttca tgcctttttc atatttaccg
    35761 gatactacgc gcataaacgc cacgcggtcg cggtgtttcg ggtccatgtt ggcctgaatt
    35821 ttaaatacga agccggtaaa tttgtcttcg ctcgcttgta cggtacgggt atcagtctga
    35881 cgcggcatcg gcgcaggtgc ccactccacc aggccatcca acatatgatc gacgccgaag
    35941 ttacccagcg cagtaccgaa gaataccgga gtgatttcgc ccgcaaggaa cagctctttg
    36001 tcgaactcgt tagacgcgcc tttaaccagt tccagttcgt cacgcagctg ctgtgccaga
    36061 tcttcaccaa ccgcagcatc gagatccggg ttattcagcc ctttaacaat gcggacttcc
    36121 tgaatggtgt gccctttacc gctctgatag agataggttt catctttata aaggtggtaa
    36181 acgcctttaa acagcttgcc gcagccaatt ggccaggtga tcggtgcgca gccaattttc
    36241 agctcgttct caacttcatc gagcaattcc atcgggtcgc ggatatcacg gtcaagtttg
    36301 ttcataaagg tgaggatcgg cgtgtcgcgc agacgggtaa cttccatcag cttacgggta
    36361 cgatcttcaa caccttttgc ggcgtcgata accatcaggc agcagtccac cgccgtcagg
    36421 gtacgatagg tatcttccga gaagtcttca tgccccgggg tgtcgagcag gttaaccagg
    36481 caatcgtgat acggaaactg catcacagac gtagtaatgg agatcccacg ctgcttttcc
    36541 atctccatcc agtccgactt agcgtgctgg ttggaaccac ggccttttac tgtaccggcg
    36601 gtctgaatgg cctgtccgaa cagcagcacc ttctcggtga tggtagtctt accggcgtcc
    36661 gggtgagaaa taatggcaaa agtgcggcgc ttcgccacct cttgcaaata aggagacaac
    36721 gtcataattc tttcttcttg agtaagcggc atcgcgccgc gcttgttgaa aacgaaaaat
    36781 tgcggctatt ttacccatca acggggggga ggcaatcagt gtttacacag gagctgctcc
    36841 agttcatgca acgaagaaac ggtccaggtg ggcgcgatgc cttctggttg ctcgcgatgg
    36901 tgtgcattca gccagcaggt cgcaagcccg gcgttgatgc caccgagaat atcggactcg
    36961 gcagtgtcgc caaccatcag cacgcgtgaa cggtcaggat tgcccgcctg ttccagcgca
    37021 taatcgaata ttttcttatt cggtttagca acgccaactt cttcggaaat caccagcaaa
    37081 tcgaagtaat cacgcaggcc cgtccgttcc agacgcactt gctgcaaggc gctaaaaccg
    37141 ttggtgatga tgccgatttt agcgttgcca cgaatggcgt taagcagaga aaccgcgccc
    37201 ggcaacggcg tacagatttc cgccatcgca ttaataaagg catcgttaag caagcctgct
    37261 tcaactttca gccgttcggc ccagctctca aaccgcccgt gctgaagctg taatgaagtg
    37321 atcgcgccgt tttgataatc cacccacagt ggcttgttaa cggcctgata gtcctgaaaa
    37381 tcttcagcgg taaaggtgac gctgtaatca agaaacatcc gctgcaggcc ggtgaatgag
    37441 tcaaaggtaa acagcgtttc atcggcatca aagaaaatcc agtcccactt cattattcca
    37501 ccttgtctta catactgatt ggcaacgcca tgatgatggc gtcttcgcga ccgtccgtgg
    37561 tggggtagta attgcggcga atcgtcgcct cgttaaagcc taaactttcg tacagggcaa
    37621 tggcggcagc gtttgaagca cggacttcca gccatagtgt cgccacgccg cgtttttcca
    37681 gttcgtcgat cagatgttcc agcagcaccc ttcccaatcc ctgacgctga tagtcaggat
    37741 cgaccgcaat attgaacaat gtagcttcat ccagcaccac ttgcgtaatc gcaaacgccg
    37801 ccattttgcc gttttgcgtt aactgaaagt tgagataacg ctcgccctgg ttgctggcaa
    37861 aggttttttc actccacgga aaggcgtggg cgcgttgttc aatgtggtaa gccgctggta
    37921 aatcagtcgt ttcgagggaa gaaatcgtgt tcatatgtgc aaatctgttg ccataacgcg
    37981 gcgcgtgccg ttgggtttgc ccgtaattcg gtgagcgccg gtgatgccac ctgagcgcct
    38041 tccagtgata gcggttcgtc agtacccaac cgccagctgt tgcagcgact gccttgcggc
    38101 agcatcgcga ttttttctgg cgtcagttgc agcacttgat cggggctgac ggttaatgcg
    38161 cgcagaacat cgctcactaa aggatcggtc agggcgggaa gatcgtttgc caccatcacc
    38221 agacggacgt gtgccgggat cgcaatggca atctccccct gcaacgcgcc agggcgacgc
    38281 agcgaccact gggtaatgcc cagttgctgt aactgccagt ctcgtcggga tgtcatagcg
    38341 aatcgctcct gttgtcaggg gcgcaaatat agcaaattcg tcgataccgc gccaacatat
    38401 ggctataatc gccgccagta tcaattgagg agcattccat gtctgcattt accccggcaa
    38461 gtgaagtctt gctgcgtcac agtgatgatt tcgaacaaag ccgtattctg tttgccggag
    38521 acttacagga tgacctgccc gcgcgtttag ataccgcggc cagccgtgct catacccagc
    38581 aattccacca ctggcaggta ttaagccgcc agatggggga taacgcccgt ttcagtctgg
    38641 tcgccacggt ggatgacgtc gcagattgcg atacgctgat ttactactgg ccgaagaaca
    38701 aaccggaagc ccagttccag ttgatgaatt tactttctct gctgccagtg gggacagata
    38761 tttttgtcgt tggcgagaac cgcagcggcg tgcgcagcgc cgagcagatg ctggcagatt
    38821 atgcgccgtt gaataaagtc gacagcgctc gtcgctgtgg cctctatttt ggtcgtctgg
    38881 aaaaacagcc ggtatttgat gccgataaat tctggggcga atacagcgtc gatggcctga
    38941 cggtcaaaac gctgcctggc gtgtttagcc gcgacggtct ggatgtcggt agccagttgc
    39001 tgctctcgac gttaaccccg cacacgaaag gtaaagtgct ggatgtcggc tgtggcgcgg
    39061 gtgtgctttc agttgccttt gcgcgccatt cgccgaaaat tcgtctcacc ttgtgcgatg
    39121 tctctgcgcc agcggtagaa gccagccgcg caacacttgc ggccaacggt gttgaaggtg
    39181 aagtctttgc cagcaacgtc ttttccgagg tgaaaggtcg ttttgatatg atcatctcca
    39241 acccgccgtt ccacgatggg atgcaaacca gcctggatgc ggcgcaaacg ctgattcgcg
    39301 gcgcggtgcg tcatcttaat agcggcggcg agctgcgaat tgtagcgaac gccttcctgc
    39361 cttacccgga cgtgctggat gagacatttg gcttccatga agtgattgcg caaaccgggc
    39421 gtttcaaggt gtatcgcgcc attatgaccc gccaggcgaa gaaaggttaa ttatctcgcc
    39481 gaataccgtg tcggatgcgg cgtgaacgcc ttatccgacc tacaaaacct gcacgttagc
    39541 ccttcgtagg ccagataaga cgcgccagcg tcgcatctgg catctgcccc cgcatgccta
    39601 ccaaacctgc acgctagttt cctgatggac atttttccag caattacacc tccggcgata
    39661 attaactatt gacgaaaagc tgaaaaccac tagaatgcgc ctccgtggta gcaattcttt
    39721 ttaagaattg atggtatgcg aaggtggcgg aattggtaga cgcgctagct tcaggtgtta
    39781 gtgtccttac ggacgtgggg gttcaagtcc cccccctcgc accaaaaacc acgttg
//
LOCUS       DAZFXT010000033        39737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.033, whole genome shotgun sequence.
ACCESSION   DAZFXT010000033 DAZFXT010000000
VERSION     DAZFXT010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 39737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.033"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..100
                     /locus_tag="ACHKW0_001804"
     CDS             <1..100
                     /locus_tag="ACHKW0_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HFO7474649.1"
                     /translation="MTITTPTGEGVTSARTFVRLRRCVLVDAFRIV"
     gene            111..947
                     /gene="eutJ"
                     /locus_tag="ACHKW0_001805"
     CDS             111..947
                     /gene="eutJ"
                     /locus_tag="ACHKW0_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HFO7474650.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            937..2124
                     /gene="eutG"
                     /locus_tag="ACHKW0_001806"
     CDS             937..2124
                     /gene="eutG"
                     /locus_tag="ACHKW0_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HFO7474651.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGLGAVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWLCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            2241..3467
                     /gene="eutH"
                     /locus_tag="ACHKW0_001807"
     CDS             2241..3467
                     /gene="eutH"
                     /locus_tag="ACHKW0_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0034228 - ethanolamine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034229 - ethanolamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HFO7474652.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            3464..4867
                     /gene="eutA"
                     /locus_tag="ACHKW0_001808"
     CDS             3464..4867
                     /gene="eutA"
                     /locus_tag="ACHKW0_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HFO7474653.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKSLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAALIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            4879..6240
                     /gene="eutB"
                     /locus_tag="ACHKW0_001809"
     CDS             4879..6240
                     /gene="eutB"
                     /locus_tag="ACHKW0_001809"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HFO7474654.1"
                     /translation="MKLKTTLFSNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            6261..7148
                     /gene="eutC"
                     /locus_tag="ACHKW0_001810"
     CDS             6261..7148
                     /gene="eutC"
                     /locus_tag="ACHKW0_001810"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HFO7474655.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            7158..7817
                     /gene="eutL"
                     /locus_tag="ACHKW0_001811"
     CDS             7158..7817
                     /gene="eutL"
                     /locus_tag="ACHKW0_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HFO7474656.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            7830..8330
                     /gene="eutK"
                     /locus_tag="ACHKW0_001812"
     CDS             7830..8330
                     /gene="eutK"
                     /locus_tag="ACHKW0_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HFO7474657.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            8376..9428
                     /gene="eutR"
                     /locus_tag="ACHKW0_001813"
     CDS             8376..9428
                     /gene="eutR"
                     /locus_tag="ACHKW0_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HFO7474658.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(9434..10333)
                     /gene="hemF"
                     /locus_tag="ACHKW0_001814"
     CDS             complement(9434..10333)
                     /gene="hemF"
                     /locus_tag="ACHKW0_001814"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HFO7474659.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(10337..11206)
                     /gene="amiA"
                     /locus_tag="ACHKW0_001815"
     CDS             complement(10337..11206)
                     /gene="amiA"
                     /locus_tag="ACHKW0_001815"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HFO7474660.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            11420..11845
                     /gene="ypeA"
                     /locus_tag="ACHKW0_001816"
     CDS             11420..11845
                     /gene="ypeA"
                     /locus_tag="ACHKW0_001816"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HFO7474661.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            11832..12281
                     /gene="yfeZ"
                     /locus_tag="ACHKW0_001817"
     CDS             11832..12281
                     /gene="yfeZ"
                     /locus_tag="ACHKW0_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HFO7474662.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEVKE"
     gene            12342..12917
                     /gene="yfeY"
                     /locus_tag="ACHKW0_001818"
     CDS             12342..12917
                     /gene="yfeY"
                     /locus_tag="ACHKW0_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HFO7474663.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            13013..13912
                     /gene="yfeX"
                     /locus_tag="ACHKW0_001819"
     CDS             13013..13912
                     /gene="yfeX"
                     /locus_tag="ACHKW0_001819"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HFO7474664.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(13970..15271)
                     /gene="pbp4b"
                     /locus_tag="ACHKW0_001820"
     CDS             complement(13970..15271)
                     /gene="pbp4b"
                     /locus_tag="ACHKW0_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015674721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HFO7474665.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQ"
     gene            complement(15276..16700)
                     /gene="murP"
                     /locus_tag="ACHKW0_001821"
     CDS             complement(15276..16700)
                     /gene="murP"
                     /locus_tag="ACHKW0_001821"
                     /EC_number="2.7.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HFO7474666.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(16704..17600)
                     /gene="murQ"
                     /locus_tag="ACHKW0_001822"
     CDS             complement(16704..17600)
                     /gene="murQ"
                     /locus_tag="ACHKW0_001822"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HFO7474667.1"
                     /translation="MQLEKMITEGSNAASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNIGLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            17764..18621
                     /gene="murR"
                     /locus_tag="ACHKW0_001823"
     CDS             17764..18621
                     /gene="murR"
                     /locus_tag="ACHKW0_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HFO7474668.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIDVISKAQFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            18750..19541
                     /gene="ucpA"
                     /locus_tag="ACHKW0_001824"
     CDS             18750..19541
                     /gene="ucpA"
                     /locus_tag="ACHKW0_001824"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HFO7474669.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            19712..20728
                     /gene="cysP"
                     /locus_tag="ACHKW0_001825"
     CDS             19712..20728
                     /gene="cysP"
                     /locus_tag="ACHKW0_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HFO7474670.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKGKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            20728..21561
                     /gene="cysT"
                     /locus_tag="ACHKW0_001826"
     CDS             20728..21561
                     /gene="cysT"
                     /locus_tag="ACHKW0_001826"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HFO7474671.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            21561..22436
                     /gene="cysW"
                     /locus_tag="ACHKW0_001827"
     CDS             21561..22436
                     /gene="cysW"
                     /locus_tag="ACHKW0_001827"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HFO7474672.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            22426..23523
                     /gene="cysA"
                     /locus_tag="ACHKW0_001828"
     CDS             22426..23523
                     /gene="cysA"
                     /locus_tag="ACHKW0_001828"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HFO7474673.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            23657..24568
                     /gene="cysM"
                     /locus_tag="ACHKW0_001829"
     CDS             23657..24568
                     /gene="cysM"
                     /locus_tag="ACHKW0_001829"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HFO7474674.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(24571..24939)
                     /gene="yfeK"
                     /locus_tag="ACHKW0_001830"
     CDS             complement(24571..24939)
                     /gene="yfeK"
                     /locus_tag="ACHKW0_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001592994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HFO7474675.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            25044..25895
                     /gene="pdxK"
                     /locus_tag="ACHKW0_001831"
                     /gene_synonym="thiJ"
     CDS             25044..25895
                     /gene="pdxK"
                     /locus_tag="ACHKW0_001831"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HFO7474676.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            complement(25937..26446)
                     /gene="crr"
                     /locus_tag="ACHKW0_001832"
     CDS             complement(25937..26446)
                     /gene="crr"
                     /locus_tag="ACHKW0_001832"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HFO7474677.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(26487..28214)
                     /gene="ptsI"
                     /locus_tag="ACHKW0_001833"
     CDS             complement(26487..28214)
                     /gene="ptsI"
                     /locus_tag="ACHKW0_001833"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HFO7474678.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            complement(28259..28516)
                     /gene="ptsH"
                     /locus_tag="ACHKW0_001834"
     CDS             complement(28259..28516)
                     /gene="ptsH"
                     /locus_tag="ACHKW0_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HFO7474679.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            complement(28900..29871)
                     /gene="cysK"
                     /locus_tag="ACHKW0_001835"
     CDS             complement(28900..29871)
                     /gene="cysK"
                     /locus_tag="ACHKW0_001835"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HFO7474680.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            complement(30056..30817)
                     /gene="cysZ"
                     /locus_tag="ACHKW0_001836"
     CDS             complement(30056..30817)
                     /gene="cysZ"
                     /locus_tag="ACHKW0_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000103 - sulfate assimilation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HFO7474681.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            31047..32033
                     /gene="zipA"
                     /locus_tag="ACHKW0_001837"
     CDS             31047..32033
                     /gene="zipA"
                     /locus_tag="ACHKW0_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HFO7474682.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            32104..34119
                     /gene="ligA"
                     /locus_tag="ACHKW0_001838"
     CDS             32104..34119
                     /gene="ligA"
                     /locus_tag="ACHKW0_001838"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HFO7474683.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            34121..34339
                     /gene="ypeB"
                     /locus_tag="ACHKW0_001839"
     CDS             34121..34339
                     /gene="ypeB"
                     /locus_tag="ACHKW0_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HFO7474684.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(34336..35334)
                     /gene="yfeH"
                     /locus_tag="ACHKW0_001840"
     CDS             complement(34336..35334)
                     /gene="yfeH"
                     /locus_tag="ACHKW0_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HFO7474685.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSTDKA"
     gene            35424..36350
                     /gene="yfeR"
                     /locus_tag="ACHKW0_001841"
     CDS             35424..36350
                     /gene="yfeR"
                     /locus_tag="ACHKW0_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7474686.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            complement(36389..37153)
                     /gene="yfeN"
                     /locus_tag="ACHKW0_001842"
     CDS             complement(36389..37153)
                     /gene="yfeN"
                     /locus_tag="ACHKW0_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpK"
                     /protein_id="HFO7474687.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            37402..38235
                     /gene="xapA"
                     /locus_tag="ACHKW0_001843"
     CDS             37402..38235
                     /gene="xapA"
                     /locus_tag="ACHKW0_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0055086 - nucleobase-containing small
                     molecule metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HFO7474688.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            38295..39551
                     /gene="xapB"
                     /locus_tag="ACHKW0_001844"
     CDS             38295..39551
                     /gene="xapB"
                     /locus_tag="ACHKW0_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HFO7474689.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTLGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            39604..>39737
                     /locus_tag="ACHKW0_001845"
     CDS             39604..>39737
                     /locus_tag="ACHKW0_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1427 family protein"
                     /protein_id="HFO7474690.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLPGILSIVFMYVGY
                     L"
BASE COUNT         9263 a        10029 c        11001 g         9444 t
ORIGIN      
        1 catgaccatc accacgccaa ccggtgaagg ggtaaccagc gcgcgtacgt ttgtccgtct
       61 gcgtcgctgt gtattagtcg atgcgtttcg cattgtttaa ggagtaacag atggcgcacg
      121 acgaacaatg gctcacccca cgtctgcaaa cggcggcaac gctgtgtaac cagacgcccg
      181 ccgcgacgga atctccgctg tggctggggg tggatctggg cacctgcgat gtggtgtcga
      241 tggttgtcga ccgcgacggt cagccggtgg cggtatgcct cgactgggcc gatgtggtcc
      301 gcgacggcat cgtctgggat ttcttcggcg ctgtcaccat tgttcgtcgc catctcgaca
      361 cgctcgagca gcaattcggt cgccgtttta gccatgcggc gacttcattt cctccaggta
      421 cggacccgcg tatttccatc aacgtgctgg agtctgccgg gctggaagtg agccatgtgc
      481 tggatgagcc aaccgcagtc gcggatctac tgcaactgga taacgcaggc gtagtggata
      541 tcggcggtgg cactaccggc attgccatcg tgaaaaaggg caaagtgacg tactcggcgg
      601 atgaagccac cggcggacat cacatctctc tgactctcgc cggaaatcgc cgtatttcgc
      661 tggaagaggc ggagcagtac aagcgcggtc acggtgacga gatttggcct gcggtgaaac
      721 cggtgtacga aaaaatggcg gacattgtcg cccggcatat tgaagggcag ggcattactg
      781 atttatggct ggcgggcggc tcctgtatgc aaccgggcgt ggcagaactg tttcgcaaac
      841 aattcccggc gttacaggtg catttaccgc agcacagttt gtttatgacc ccgctggcga
      901 tcgccagtag tgggcgagag aaagcggagg ggctatatgc aaaatgaatt gcagaccgcg
      961 ctctttcagg cgttcgatac cctgaatctg caacgggtaa aaacatttag cgttccaccg
     1021 gtgacgcttt gcggtctggg cgcggtgagc agttgcgggc agcaagcgca aacgcgtggg
     1081 ctgaaacatc tgttcgtgat ggcagacagc tttttgcatc aggcagggat gaccgccggg
     1141 ctgacgcgca gcctggccgt taaaggcatc gccatgacgc tctggctatg tccggtgggc
     1201 gaaccgtgca ttaccgacgt gtgtgcagcc gtggcgcagt tgcgtgagtc aggttgtgac
     1261 ggggtgatcg catttggcgg cggctcggtg ctggatgcgg cgaaagccgt ggcgttgctg
     1321 gtgacgaacc cggatagcac gctggcagag atgtcagaaa ccagcgttct gcaaccgcgc
     1381 ttgccgctga ttgccattcc aactaccgcc ggaaccggct ctgaaaccac caatgtaacg
     1441 gtgattatcg acgcggtgag cgggcgcaag caggtgttag cccatgcctc gctgatgccg
     1501 gatgtggcga tcctcgacgc cgcattgacc gaaggtgtgc cgtcgcatgt cacggcgatg
     1561 accggcattg atgcgttaac ccatgccatt gaagcataca gcgccctgaa cgctacaccg
     1621 tttaccgaca gtctggcgat tggtgccatt gcgatgattg gcaaatcgct gccgaaagcg
     1681 gtgggctacg gtcacgacct tgccgcgcgc gagagcatgt tgctggcttc atgtatggcg
     1741 ggaatggcgt tttccagtgc gggtcttggg ttgtgccacg cgatggcgca tcagccgggc
     1801 gcggcgctgc atattccaca cggtctggcg aacgccatgt tgctgccaac ggtgatggaa
     1861 tttaaccgga tggtttgtcg cgaacggttt agtcagattg gtcgggcact gcgaactaaa
     1921 aaatccgacg atcgtgacgc tattaacgcg gtaagtgagc tgattgcgga agttgggatt
     1981 ggtaaacgac tgggcgatgt tggcgcgaca tctgcgcatt acggtgcatg ggcgcaggcc
     2041 gcgcaggaag atatttgtct gcgcagcaac ccgcgtaccg ccagcctgga acaaattgtc
     2101 ggactgtacg cggcggcgca ataaatgccg gatgcgacgc ttgccgcgtc ttatccggcc
     2161 tacgggattg cacatgtagg gcggataagg cgtttacgcc gcatccgcca ataaataaca
     2221 actaacaggg agtaaaggcg atgggaatta acgaaatcat catgtacatc atgatgttct
     2281 ttatgctgat agctgccgta gacaggatcc tgtcgcagtt cggcggttct gctcgtttcc
     2341 tcggtaagtt cggtaaaagt atcgaaggat caggcggtca gttcgaagaa ggctttatgg
     2401 caatgggcgc tctgggtctg gcgatggtcg gtatgaccgc gctggcaccg gtactggctc
     2461 acgtactcgg gccggtaatt atcccggttt acgaaatgct cggcgcaaac ccgtcaatgt
     2521 tcgccggaac actgctggcg tgcgatatgg gcggcttctt cctcgccaaa gagctggcgg
     2581 gcggcgacgt agcagcgtgg ctatactctg ggttaattct cgggtcgatg atggggccaa
     2641 cgattgtgtt ttccattccg gtggcgctcg gcattatcga accttctgat cgtcgttatc
     2701 tggcgctcgg cgtgctggcg ggcattgtga ccattccgat tggctgtatt gctggtggtc
     2761 tggttgctat gtactccggt gtgcagatca acggtcagcc ggtggaattc actttcgccc
     2821 tgatcctgat gaacatgatc ccggtgatca ttgtcgcgat tctggtggcg ctggggctga
     2881 aattcatccc ggaaaaaatg atcaacggct tccagatctt tgccaaattc ctcgttgcat
     2941 tgatcaccct cggtcttgcc gctgcggtag tgaaattcct ccttggctgg gaactgatcc
     3001 ccggactgga tcctatcttt atggcccctg gcgataaacc cggtgaagtg atgcgcgcca
     3061 ttgaagttat cggttctatc tcctgcgtgc tgttaggggc gtatccaatg gtgctgctgc
     3121 tgactcgctg gtttgaaaaa ccgctgatga gcgtcggtaa agtactgaat atgaacaaca
     3181 tcgcggcagc cggcatggtg gcaacgcttg ccaacaacat cccgatgttc ggcatgatga
     3241 agcagatgga tacccgcggc aaagtcatca actgcgcctt cgccgtttcc gctgctttcg
     3301 ccctgggcga ccacttaggc ttcgccgctg ccaacatgaa cgccatgatc ttcccgatga
     3361 ttgtcggcaa gttgatcggc ggcgtaacgg cgattggcgt ggcgatgatg ctggtgccaa
     3421 aagaagacgc gaccgcgact aaaaccgaag cggaggcaca atcgtgaaca ctcgccagct
     3481 attgagcgtc ggtatcgata tcggcaccac caccacccag gtgattttct cccacctgga
     3541 actggttaac cgtgcggcgg tgtcgcaggt gccgcgctac gaattcatta aacgcgaaat
     3601 tagctggcaa agcccggtat tctttacccc tgtcgataaa cagggtggtt taaaagaagc
     3661 ggaactgaaa tcgctgattt tagagcaata tcaggctgcg ggcattgcgc cggaaagcgt
     3721 tgattctggt gccatcatca tcaccggtga aagcgcgaaa acccgcaatg ctcgcccggc
     3781 ggtgatggcg ctctctcagt cgctgggcga ttttgtcgtt gccagcgccg ggccgcacct
     3841 cgaatccgtg atcgccggtc acggagccgg ggcacaaacc ctttctgaac aacggctgtg
     3901 tcgggtactg aatatcgaca tcggcggtgg caccgcgaac tacgccctgt tcgatgccgg
     3961 aaaaatcagc ggcactgcct gtctcaacgt cggtggtcgc ctgttggaaa ccgacagcca
     4021 ggggcgcgtg gtttacgctc ataaaccggg gcagatgatt gtggatgagt gcttcggtgc
     4081 aggcactgac gcccgttcgc tgaccggcgc gcagctggtg caggttaccc ggcggatggc
     4141 ggcgctgatt gtcgaagtga ttgacggaac gctttcgccg ctcgcgcagg cattgatgca
     4201 aaccggtttg ctgcccgcag gtgttacgcc cgaaatcatt acgctttctg gaggcgtggg
     4261 cgaatgttat cgccaccagc ccgccgaccc gttctgtttt gccgatattg gcccacttct
     4321 ggcaacggcg ctgcatgacc atccgcgcct gcgtgagatg aatgtgcagt ttccggcgca
     4381 aaccgtacgc gccacggtga ttggcgcggg tgcacatacc ctttcgctct ctggcagcac
     4441 aatctggctg gagggcgtac aactgccgct gcgcaatttg ccggtggcga tcccgattga
     4501 tgaaacggat ctggtgagtg cctggcaaca ggcgctgctt cagctggatc ttgatcccaa
     4561 aactgacgcg tacgtgctgg cgcttcccgc ctcgctgcct gtgcgttacg ccgcggtact
     4621 gacggtcatc aacgcgctgg tcgatttcgt cgcgcgtttt ccgaatccgc atcccctgct
     4681 ggtggtggcc gggcaggact ttggtaaagc tctgggcatg ttgttgcgcc cacagctaca
     4741 acaactcccg ttggcagtca ttgacgaagt gattgtccgc gcgggggact atatcgacat
     4801 tggtacgcct ctttttggcg gatcggttgt gccggtgacg gtgaaatcac tcgcatttcc
     4861 ttcctgaggg aacgacttat gaaactaaag accacattgt tcagcaatgt atatcagttt
     4921 aaggatgtaa aagaggtgct ggctaaagcc aacgaactgc gttcggggga tgtgctggca
     4981 ggcgttgcag cggcaagttc acaggagcgc gtggcggcaa agcaggtgtt gtcggaaatg
     5041 accgtagcgg acatccgcaa taatccggtg attgcctatg aagatgactg cgtgacgcgg
     5101 ctgattcagg acgatgttaa cgaaacggcc tacaaccaga ttaaaaactg gagcatcagc
     5161 gaactgcgtg agtatgtgct gagcgatgaa accagcgtgg acgacattgc ctttacccgc
     5221 aaagggctga cctcggaagt ggtcgcggcg gtagcgaaga tttgctccaa cgcggacctg
     5281 atctacggcg cgaagaaaat gccggtaatc aaaaaggcca ataccaccat tggtattccg
     5341 ggcaccttca gcgcccgttt gcagccaaat gacacccgtg acgacgtgca aagtatcgcc
     5401 gcgcaaatct acgaagggct ttccttcggg gtgggcgatg cggtgatcgg cgttaacccg
     5461 gtgactgacg acgtggaaaa cttaagccgc gtgttggaca ccatttacgg cgtgatcgac
     5521 aaattcaaca tcccaactca gggctgcgta ctggcgcacg tcaccaccca gatcgaagcg
     5581 attcgtcgcg gcgcgcctgg cggactgatt ttccagagta tctgtggcag cgaaaaaggg
     5641 ctgaaagagt ttggcgtgga actggcgatg ctcgacgaag cgcgcgcagt gggtgcagag
     5701 ttcaaccgta tcgccgggga aaactgcctc tacttcgaaa ccggacaagg ctctgcgcta
     5761 tccgctggcg ctaacttcgg cgctgaccag gtgacgatgg aagcacgtaa ctatgggctg
     5821 gcgcgtcatt acgatccgtt tatcgtcaac accgtggtcg gctttattgg gccggagtat
     5881 ctctacaacg accgccagat tatccgtgct ggcttagaag atcactttat gggcaagctg
     5941 agcggcatct ctatgggctg tgactgctgt tacaccaacc acgctgacgc tgaccagaac
     6001 ctcaacgaaa acctgatgat cctgctcgcc accgcaggct gcaactacat catggggatg
     6061 ccgctgggtg atgacatcat gctcaactac cagaccaccg cattccacga taccgccact
     6121 gtgcgtcagt tactcaacct gcgtccgtca ccggagtttg aacgctggct ggaaagcatg
     6181 ggcattatgg caaacggtcg cctgaccaaa cgggcgggcg atccgtcact gttcttctga
     6241 tgacgcggag ataacacatc atggatcaaa aacagattga agaaattgta cgcagcgtga
     6301 tggcgtcaat gggacaaacg gcccccgcgc cgtcagaagc aaagtgcgcc accaccaact
     6361 gtgcggcacc ggtgacctcg gaaagctgtg cgctggattt aggttccgct gaagcaaaag
     6421 cgtggattgg tgttgaaaac ccgcatcgcg cagacgtatt aacagaactg cgccgcagca
     6481 ccgtggcccg cgtttgtacc ggtcgtgccg gtccgcgtcc acgtacccag gcgctgctgc
     6541 gtttcctggc cgatcactct cgttcgaaag ataccgtact gaaagaagtg ccggaagagt
     6601 gggtgaaagc gcagggcttg ctggaagtac gctctgaaat tagcgacaaa aatctctacc
     6661 tgactcgccc ggatatgggc cgccgcctgt gtgcagaagc tgttgaagcg ctgaaagcac
     6721 agtgtgttgc taatccggac gtacaggtcg ttatttctga tggcctgtca actgatgcca
     6781 tcaccgtgaa ctacgaagag atcctgccgc cgctgatggc gggcctgaaa caggccgggc
     6841 tgaaagtcgg cacgccattc tttgttcgtt atggtcgcgt gaagattgaa gatcagatcg
     6901 gtgagatcct cggcgcgaaa gtggtgatcc tgctggtggg ggaacgtccg gggttagggc
     6961 agtcagaaag cctctcctgc tacgccgttt actcgccgcg tatggcgacc accgttgagg
     7021 ccgatcgcac ctgtatctct aacattcacc agggcggcac gccgccggtt gaagccgcag
     7081 ccgtcattgt ggatttggcg aaacgtatgc tggagcagaa agcatccggc atcaacatga
     7141 cccgataagg aggcatcatg ccagctttag atttgattcg accgtcggta accgccatgc
     7201 gggtgattgc ctccgttaac gctgaatttg cgcgtgaact aaaattgccg ccgcatattc
     7261 gtagcctcgg gctgatttct gctgattccg atgacgtcac ctacattgcc gctgatgaag
     7321 cgaccaagca ggcgatggtg gaagtggtat atggtcgctc gctgtatgcg ggcgctgcac
     7381 acggcccgtc accgactgcc ggtgaagtgc tgattatgct tggtgggcca aacccggcgg
     7441 aagtgcgtgc cggtctggat gcgatggttg cacatattga aaatggtgca gcattccagt
     7501 gggccaatga cgcagaaaac accgccttcc tggcgcatgt ggtttcgcgt accggttctt
     7561 atctctcatc aaccgccggg atcacgcttg gcgatccgat ggcgtatctg gtggcaccgc
     7621 cgctggaagc gacctatggc attgatgcag cgttgaaatc tgccgacgta cagctggtga
     7681 cctatgtccc gccaccgtct gaaaccaact actcggcagc atttttaacc ggtagccagg
     7741 ccgcgtgtaa agcagcctgt aacgccttta ccgatgcagt gctggaaatc gcgcgtaatc
     7801 caatccagcg tgcgtaacgg aggttgccga tgatcaatgc actgggattg ctggaagtgg
     7861 acggcatggt cgccgcgata gatgcagcgg atgccatgct caaagcagct aacgttcgtc
     7921 tgctcagtca cgaagtgctt gaccctggtc gcttaacgct ggtggtggaa ggcgatctgg
     7981 cggcgtgtcg tgcggcgctg gacgcaggtt gtgctgccgc gatgcgtacc gggcgtgtca
     8041 tcagccgcaa ggagatcggt cggccagacg atgacaccca gtggctggtt actggcttta
     8101 accgccagcc gaagcaaccc gtaagggaac ccgacgcgcc agttatcgtc gcggaatctg
     8161 ctgacgagtt gttggcgctg ttaacatcag tacgtcaggg aatgacggca ggagaagtgg
     8221 ctgcccactt tggctggccg ctggaaaaag ccagaaatgc gctcgaacag ctcttttctg
     8281 ccgggacgtt acgtaaacgc agtagtcgtt atcgtctcaa gccccattaa cctgtcggag
     8341 gtgccgggtg tcctacaaca cccggcatta acatcatgaa aaagacccgt acagccaatt
     8401 tgcaccatct ttatcatgaa cccttacccg aaaacctgaa gctcacgccg aaggtcgaag
     8461 tggataatgt tcatcaacga cagacaacgg atgtctatga acatgcttta acgattaccg
     8521 cctggcagca gatttacgat cagctgcatc cgggcaagtt tcatggtgaa tttacggaaa
     8581 ttctactcga tgatattcag gtttttcgtg aatacaccgg tctggcgctg cgtcagtcgt
     8641 gcctggtctg gccgaactcg ttctggtttg gcattccggc gacgcgcggt gagcagggat
     8701 ttatcggttc gcaatgtctg ggaagcgcgg aaatcgccac ccgccctggt ggcactgaat
     8761 ttgaactgag cacgccggat gattacacga tcctgggcgt ggtgctttct gaagatgtca
     8821 tcacccgaca ggctaacttt ttgcataacc cggatcgggt attacatatg ctgcgtagcc
     8881 agtcggcgct ggaagtgaaa gagcagcata aagccgcgct gtggggcttt gtccaacagg
     8941 cgctggcgac gttttgcgag aacccggaaa atctccatca gcctgcagtg cgaaaagtgc
     9001 tgggggataa tttgctaatg gcgatggggg cgatgctgga agacgcgcaa ccaatggtga
     9061 cggcggaaag catcagtcat cagagttacc gtagattact ttcccgcgcc cgtgaatatg
     9121 tgctggaaaa tatgtctgag ccggtgacgg tgctggactt gtgtaatcaa ctgcatgtca
     9181 gtcgccgcac gctacaaaac gcgtttcacg ctattttagg cattggccca aacgcgtggc
     9241 tgaaacgcat tcgcctgaac gccgtacgcc gcgaactgat aagcccgtgg tcgcaaagca
     9301 caacggtaaa agacgccgcc atgcagtggg gattctggca tctggggcaa tttgccacgg
     9361 attaccagca gctgtttgcc gagaagccgt cgttgacgtt gcatcagcgg atgcgggaat
     9421 gggggtgagg gagttacacc caatccctga ccttaataaa ctcacttaac gccgcttctg
     9481 ggctgccatc ttttggctga taatcatact cccagcgtac cagtggcggc attgacatca
     9541 ggatcgactc ggtgcgcccg ccagtttgca ggccaaacag agtgccgcga tcccagacca
     9601 gattgaactc gacataacga ccgcgacggt agagctgaaa attgcgctcg cgctcgccgt
     9661 aggccatcgc tttacgtcgc tctacaattg gtaaataagc gtcggtgtag cctttgccta
     9721 ccgcctgcat aaaggcaaaa cagtggtcga aatctggcgt gttcagatca tcaaagaaca
     9781 gcccgccaat accgcgctgt tcgttgcgat gtttgaggta gaagtaatcg tcgcaccact
     9841 ttttgtaacg gggataaacg tcttcaccaa atggcaggca caggtcacgg gcggtgcggt
     9901 gccagtgaat ggcgtcttct tcaaaaccat agaaaggggt taaatcgaag ccgccgccaa
     9961 accaccagac gggctctgca cccggttttt cggcaataaa aaaacgcaca ttcgcgtggc
    10021 tggtgggaac atacgggtta tgcggatgca ctaccagtga aacgcccatc gcctcgaaac
    10081 tgcgcccggc aagttccggg cgatgagcgg tggcggaagc tggcatcgcc tcgccgtgga
    10141 cgtgagaaaa gttgacgcct gcctgttcga aaacaccacc attacgcaac acccgactac
    10201 gcccgccgcc gccagcttcg cgctgccaac tatcttcgac aaattctgcg ccatcgacgg
    10261 cggtcagctg ctgacaaatc gtatcctgaa ggttgagcag aaactgttta acctggtgtg
    10321 cgtcgggttt cataacttat cgctttttcg aatgtgcttt ctggttgtcg aaccagtgga
    10381 aataactgat cacgccttca gcaatcgctg tggcgatttt ctgacgaaac gccgccgtgc
    10441 ctaacagccg ctcttcttcc gggttggtga taaacgaggt ttccaccagc accgaaggaa
    10501 ccgacggcga tttcaacacc acaaacgcgg cttgttcggt gttgcggctg tgcagtttat
    10561 gcaccggctt aatctttttc agaatatgcg agccgagcgt caggctgttt ttaatagtat
    10621 cggtttgcac cagatcaaac agcacttgct gcaataggtg atccttgtca gtcgcctttt
    10681 taccggcaac ttcatcggcg cggttttcgc gttcagacag gtatttcgcc attgcgctac
    10741 ttgccccacg gttagagagg gcaaataccg aagcaccggc agctttcggg ttggtaaagc
    10801 catcggcatg aattgacata aacagatctg cgccatgttt atgggcgatt tcaacgcgat
    10861 cgtaaagtgg gataaacgta tcgccagaac gcgttaaacg cgcatcaatc ccatgattac
    10921 gcaaaatgga acggacgttt ttagcaatcg ccagcaccac atgtttttct ttcgaaccgt
    10981 tgcgtccgat cgctccggta tcaattccgc cgtggcctgg atcgagaaca acgacacgtt
    11041 tgccgccaga ttttttggct ttcggcttgc tgtgtccgtt gctggttttt aaaggttcgt
    11101 ctttggcgat ggcttgcgac attcctgaca acgtcagggc agccaaaccg gctttcagca
    11161 cctggcggcg cgaagtgagt gtttttagtg gtttaaaagt gctcatacgg cctgagttgt
    11221 aataataaag ttccagatgt tatatcgtat cgcgtaatcc gttacgacgg tttgatgttg
    11281 agaattgttt tacttttcat ttcaatagtt gacaactccc cattatgcca ctttttcaac
    11341 cgattttcgt cagatattgg ctaattcgac cgttcacgcc ataatcagcc ttttaacccg
    11401 caaaaaggta acgaatacca tggagatacg cgtatttcgc caggaagatt tcgaagaggt
    11461 catcaccctt tgggagcgtt gcgacttgct gcgtccgtgg aacgatccgg aaatggacat
    11521 cgagcgtaag atgaaccatg acgtcagttt gtttttggtc gctgaggtaa acggtgaagt
    11581 ggtcggaacg gtgatgggcg gttatgacgg gcatcgcggg tctgcttatt atcttggcgt
    11641 gcatccagag tttcgtgggc gtgggattgc caatgcgttg cttaatcggc tggagaaaaa
    11701 gctgattgct cgtggctgcc cgaaaattca gatcaatgtg ccggaagata acgacatggt
    11761 gctcggaatg tatgaacgcc tgggatatga acacgccgac gtgctgagtc tgggtaagcg
    11821 tttgattgaa gatgaagagt actgagttcc atcctgtcca ttatgatgcg cacggacgcc
    11881 tgcgtttacc cttgctcttc tggcttgtgc tattgcttca ggcgcgaacc tgggtgctgt
    11941 ttgtcattgc cggtgcgtcg cgtgagcagg gtactgcgct gctaaatctg ttttatcccg
    12001 atcacgataa tttctggctg gggctgattc ctggcattcc ggcggtgctg gcgtttttgt
    12061 tgagcggtcg gcgggctaca tttcctcgca cttggcatgt gctctatttt ctgttgctgt
    12121 tggcgcaggt ggttttactt tgctggcaac cgtggctgtg gctgaacggt gaatccgtta
    12181 gcggtatcgg tctggcgctg gtagtggcgg atattgtggc gttaatctgg ctgctgacca
    12241 atcgacgttt acgcgcttgt tttaatgaag taaaagaata acggcacttt ttggtgaatt
    12301 tgcactccaa gcaacgttat tgaataacca aaggaagtga catgaaatcg ctgcgtttaa
    12361 tgttatgcgc tatgccattg atgctgaccg gctgttccac gatgtcgtca gttaactggt
    12421 ctgccgctaa cccgtggaac tggtttgggt cgtccaccaa agtgagcgag cagggcgttg
    12481 gtgaattaac ggcgtccaca ccactgcaag aacaagccat tgccgatgcg cttgatggcg
    12541 attatcgcct gcgcagcgga atgaaaaccg cgaacggcaa cgtggtgcgc ttttttgaag
    12601 tgatgaaggg cgacaacgtg gcgatggtga ttaacggcga tcaaggcacg atcagtcgca
    12661 ttgacgtgct ggatagcgat attcctgctg acaccggtgt taaaatcggt acaccgttta
    12721 gcgaccttta cagcaaagca tttggcaatt gccaaaaagc tgatggtgat gataatcgtg
    12781 ccgtcgaatg taaagccgaa ggcagtcaac atattagcta ccagttcagc ggggaatgga
    12841 gtggtcctga agggttaatg ccttcggacg ataccctgaa aaactggaaa gtcagtaaaa
    12901 ttatctggcg gcgttaattt gcgtctgaac aaaccgccgc cgcgaaaaaa cacgtaaaat
    12961 agcgctcaac aatgccacgg attgcgtggc attattattt tcaggaggaa caatgtctca
    13021 ggttcagagt ggcattttgc cagaacattg ccgcgcggcg atttggatcg aagccaacgt
    13081 gaaaggggaa gttgacgccc tgcgtgcggc cagtaaaaca tttgccgaca aactggcaac
    13141 ttttgaagcg aaattcccgg acgcgcatct tggtgcggtg gttgcctttg gtaacaacac
    13201 ctggcgcgct ctgagcggcg gcgttggggc agaagagctg aaagattttc cgggctacgg
    13261 taaaggcctt gcgccgacca cccagttcga tgtgttgatc cacattcttt ctctgcgtca
    13321 cgacgtaaac ttctctgtcg cccaggcggc gatggaagcc tttggtgact gcattgaagt
    13381 gaaagaagag atccacggct tccgttgggt tgaagagcgt gacctgagcg gctttgttga
    13441 cggtacggaa aacccggcgg gtgaagagac gcgtcgcgaa gtggcggtta tcaaagacgg
    13501 cgtggatgcg ggcggcagct atgtgtttgt ccagcgttgg gaacacaacc tgaagcagct
    13561 caaccggatg agcgttcacg atcaggagat gatgatcggg cgcaccaaag aggccaacga
    13621 agagattgac ggcgacgaac gtccggaaac ctctcacctg acccgcgttg atctgaaaga
    13681 agatggcaaa ggactgaaga ttgttcgcca gagcctgccg tacggcaccg ccagtggcac
    13741 tcacggtctg tacttctgcg cctactgcgc gcgtctgcat aacattgagc agcaactgct
    13801 gagcatgttt ggcgataccg atggcaagcg tgatgcgatg ttacgtttca ccaaaccggt
    13861 cactggcggc tattacttcg cgccgtcgct ggacaagttg atggcgctgt aagcgtttct
    13921 gattcgatga tgaaaggcca ggcagtaaca ttactgactg gcctttttat tactgcttta
    13981 acgccgcata tacctgatca accacccaac cataagttgc aattggcaac tgaccgcttt
    14041 cgaacatatt gggattcttt tgcggatcgg caaccggtga atgtggcttg ttacttaaca
    14101 tcacaatcgc catatgattc accggatcga taacggtcac cgttccggtc cagccagtgt
    14161 gaccgtaggt ttgcgggctt gccagcgtgc caaacgtcgg cgtcatggtg gcattaccat
    14221 ttacgcgcca gccgaggcca aaagtggcat cttccttaga gctggtggtg aacatcttca
    14281 ccgtttccgc actgaacagc tgcacatcgc catagccccc gccgttcagc atcgtttgca
    14341 ttaacaccgc aatatcgccg gtattggaaa acaaacctgc gtgcccggaa acgccgccca
    14401 tcgaataaaa ggctttttca tcgtgcacct gaccccagag agtggaggtg cggatattcg
    14461 ggaaatggat cacgccatcg cgggtattgc cgttcaattc cgtggcggca atttgttgcg
    14521 gtttaaagcc tttcagcagc gggttaaata ccgtatgtgt caggccgagc gggcgataaa
    14581 tcgactcttc aacatagcgg tcaagcggct gaccggtgac cgactcgacg ataaatccaa
    14641 gcagcatata atcgacatca ctgtaaatat gcttgctgcc gggctgatac tctagcggcg
    14701 tgcgcttgat catctccagc gtctggcctt tatcctggga atataacgcg cccgcgaccg
    14761 ctttattcgg gtattgcgga tctgccggga aaccgccgct gtgatgcagc aggtcagaaa
    14821 tccgcagggt gtttttgcct ttaatggtgt catttggact atcggcaaac cccggaatat
    14881 atttcgcaat ccgatcgtca ggatgcagtt tgccttcgct catcagcttt tgcagggcga
    14941 agttcgtggc gtacattttg gtgtttgagg ccaggtcata cagcgtcccg gtggtggctt
    15001 tgacaggctg ttccatcaac acgctgccat cgtacttttt cgccgctccc caggctttgc
    15061 gatacacaat ctgattatct ttaatgatca gcaggtttac gccgggataa ccggcgtcca
    15121 cttgctggct aatccagcga tccatctggt taagccgttc gacgttgaac cctgctttct
    15181 ctggcgaact ttctgtcaga acagggtatt ttgcggcgct gacgctacaa ctaacagcca
    15241 gcagagaaag atagagcatt gtccgtttca tgatatcagt ccagattgac gttacggcag
    15301 ccaaagagcg tggtgaaaat aaacccgcaa acccatgcca ccagaatccc gccagcataa
    15361 accgccattg ccggaaggat gccttgtgcg gaagtcatca gcggcagcgc caccagacca
    15421 gacggcccaa aggcgctgtt taagcccatc ggcagacccc accaggctat caggccgata
    15481 aacaaaccgc ccgctgcgcc gcctaaacag gcggtaataa acggtttcat gcggggcagg
    15541 gtgacgccgt aaatcaacgg ttcaccaacg cccagcagac cgggaataat cgccccgcgt
    15601 acctgactgc gtaatgcact gtgcggttgc gcccgccagt agagtgccag cgccgcgccc
    15661 acctggcccg cgcctgccat tgaaaggatg ggaaataagc tgttgaatcc ctggctgtcc
    15721 attaacgcga ggtaaacagg aataaagccc tgatgcacgc caaataccac ggcgatcagg
    15781 aacagcccgg ctaaaaccgc acaaccgaac ggattactgt tcaggtgcat aaacagccat
    15841 gacatacctt cgaataacca gccacccagc ggcataatga tcaggtacgc aagcgtagcg
    15901 gtgatcagca gggtgattaa cgaggtcagc agcatgtcga gatcgtccgg cataaagcgg
    15961 cgcaccatgc cttcaatgcg ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg
    16021 ccgcgcggat cgatgggcag accaaagaaa tcgtgaaaac cggcgtagta accggtggtt
    16081 gcagcggggt tataaccgag caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg
    16141 ccgccaaatg cctgggcggc gttatagccc accagaatca ccaggaaagt gaacaaacct
    16201 ttgctgaaca ccttcataaa attcagcgcg tcgggtagcg tgccctgagc atctgccgga
    16261 acgtgcatca ccgtggcaat taacgtcgct atccccagca gcagaccggc ggcaataaaa
    16321 ccggggatca gcggcgtgaa gatggtggcg aatttggcga gaaattgttg cacgccggag
    16381 gtttgtttgg cttttaactg acgtttgttc tgggcggcga tttctgcggc atcctgtacc
    16441 ggtgcctcgc ccagcagctc gctcatcgct tttgcggcac gatgcgcttt accaggccca
    16501 aaaacgacct gcacctggtc gctggtcaaa atgacgccct tcacgccttc cagcgttttg
    16561 atattgggat caaccagtga actgtcatgt acacccagac gcaggcgcgt catacagtta
    16621 ccacaactgg cgatatttcc cggtccgccg acacgggtaa gaatggtgtt cagaagttca
    16681 ctgctgatct ctttggccat gggttattcc ttgtctaaaa cctgacgaat aaagccgccg
    16741 tgttgatcca ggcgtttttt agcttctgcg gcatcgagat ttttcagcac catcacaatg
    16801 gccgttttac agttgcgctc gcaggcaatt aacgccgctt ccgcttgttc ggcattacat
    16861 ccggtggcgt ttttaacgat attgacctgg cgcacatgca gtttttcgtt ggtggcgacc
    16921 acatcgacca tcaggttgcc gaacactttg ccggatttaa tcatcagccc ggtggagagc
    16981 atattgagca ccagtttctg cgctgtacct gctttcatcc gcgaagaacc ggtaaccact
    17041 tcggcaccta caatcggtgt aatggcaaac tctgcggtgg ttgaaacggc gctccccgga
    17101 ttacaggaaa ttcccactgt gcggcagccg agctggcgtg cgtattccag tccggcaatt
    17161 acatacggcg tgcgaccact ggccgcaatg ccaaccacca catcctgcgc ggttaagccg
    17221 atgtttttca gatcgttaac accaccttcc cggctatctt cagcgccttc caccgcatgc
    17281 tgaatggcat attcgcctcc agcaatcaaa ccaaccacca aacccggttt cacgccgtag
    17341 gtgggcggac attcgctggc atccagaatc cccagacgac cggatgttcc cgcaccgaga
    17401 taaatcagac gaccgccgcc gcttacctgg gcgtggataa catcgatcgc cgcggcgata
    17461 tccggcagta cgcgctcaac ggcaagcggt acggttttat cttcatcgtt gataatccgg
    17521 cacatttcca gcgttgatac gcggtcaatt tcagccgagg cggcgttcga gccttcggta
    17581 atcatctttt caagttgcat cggtggtgac cttactattt acgatcaaat aattgcagat
    17641 gtatggaatc atatatcccg aattgacaga cttaaaggaa tcattgagtc tacactcgca
    17701 gagtgtgaag agcgtcataa gaacttaagc ggaatttttg ctgccagccc aggagagtgg
    17761 aagatgttgt acctgacgaa aatacgcaat gcggaaagtg aatttaccga gaacgagcaa
    17821 aaaattgctg actttctgcg ggccaacgtg agtgagctga aatccgtctc ttcgcgcaag
    17881 atggcgaaac agctcggcat cagccagtcg agtattgtga agtttgccca gaagctcggc
    17941 gcgcaaggat ttactgaatt gcgtatggcg ttaattggcg aatacagtgc cagccgggag
    18001 aaaacgaatg ccacggcgct gcacctgcac agttcgatta ccagtgatga ttcgctggaa
    18061 gtgattgccc gcaaactgaa tcgcgaaaaa gagctggcgc tggagcaaac ctgcgcgttg
    18121 ttcgactacg cgcggctgca aaaaatcatt gatgtcatca gtaaagcgca atttattcag
    18181 ataacgggcc tgggcggatc ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa
    18241 attggctatc gcgtggcctg cgaagccgac acgcacgtac aagccaccgt ttcccaggcg
    18301 ttgaaaaaag gcgatgtaca aatcgctatc tcttacagcg gcagtaaaaa agagattgtc
    18361 ttgtgcgcag aagcggcacg aaagcagggg gcaacggtga ttgccattac ttctctggct
    18421 gactctccgc tgcggcggct ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa
    18481 tggcgtagct cgtcgatgtc tacccgcacg gcgcaaaatt ctgttaccga tttactgttt
    18541 gttggtcttg tgcaactcaa tgatgtcgag tcattgaaaa tgattcagcg cagcagtgag
    18601 ctaacacaac gcctgaaatg atgcataaag cagcgactgg attgagattt tcctgaatta
    18661 gtgagctgat ccgcagcaat attttgttta tcctgtattt tcagagggaa tggagtgtaa
    18721 cgctctgtat taacaaggag agcattaaaa tgggtaaact cacgggcaag acagcactga
    18781 ttacgggcgc attgcaggga attggcgaag gaattgccag aacttttgcg cgtcatggcg
    18841 caaacctaat cttgctggat atctcccctg agatcgaaaa gctggcggac gaactgtgtg
    18901 gtcgtggtca tcgctgtacg gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg
    18961 cagctatcaa acgcgcgaag gaaaaagaag ggcgcattga tatcctggtg aataacgcag
    19021 gcgtttgtcg tctgggcagt ttcctcgata tgagcgatga agatcgcgat ttccatattg
    19081 acatcaatat taaaggcgta tggaacgtca cgaaggcggt gctgccagag atgattgcgc
    19141 gcaaagatgg tcgcattgtg atgatgtctt cagtcactgg tgatatggtg gccgatcctg
    19201 gcgaaacggc gtacgcctta acgaaagcgg cgattgttgg cctgacaaaa tcgctggcgg
    19261 tggagtacgc gcagtctggt attcgcgtta acgccatttg cccgggatac gtgcgcacac
    19321 caatggcgga aagcattgcc cgccagtcga acccggaaga tccagagtcg gtgctgactg
    19381 aaatggcgaa agcaatcccg atgcgtcgcc tcgccgatcc gctggaagtc ggcgaactgg
    19441 cggccttcct cgcatcggat gaatccagct atttaaccgg tacacagaat gtgattgatg
    19501 gcggcagcac actgccggag acggttagcg tcggtatctg attcacctct gtttcctccc
    19561 tgcatttgtg gggaggattt cgttttaaac ccggttctta ttcccttttc aacttccaaa
    19621 tcaccaaacg gtatataaaa ccgttactcc tttcacgtcc gttataaata tgatggctat
    19681 tagaaagtca ttaaatttat aagggtgcgc aatggccgtt aacttactga aaaagaactc
    19741 actcgcgctg gtcgcttctc tgctgctggc gggccatgta caggcaacgg aactgctgaa
    19801 cagttcttat gacgtctccc gcgagctgtt tgccgccctg aatccgccgt ttgagcaaca
    19861 atgggcaaaa gataacggcg gcgacaaact gacgataaaa caatctcatg ccgggtcatc
    19921 aaaacaggcg ctggcgattt tacagggctt aaaagccgac gttgtcactt ataaccaggt
    19981 gaccgacgta caaatcctgc acgataaagg caagctgatc ccggccgact ggcagtcgcg
    20041 cctgccgaat aatagctcgc cgttctactc caccatgggc ttcctggtgc gtaagggtaa
    20101 cccgaagaat atccacgatt ggaacgacct ggtgcgctcc gacgtgaagc tgattttccc
    20161 gaacccgaaa acgtcgggta acgcgcgtta tacctatctg gcggcatggg gcgcagcgga
    20221 taaagctgac ggtggtgaca aaggcaaaac cgaacagttt atgacccagt tcctgaaaaa
    20281 cgttgaagtg ttcgataccg gcggtcgtgg cgcaaccacc acttttgccg agcgtggcct
    20341 gggcgatgtg ctgattagct ttgaatcgga agtgaacaac atccgtaaac agtatgaagc
    20401 acagggcttt gaagtggtga tcccgaaaac caatattctg gcggaattcc cggtggcgtg
    20461 ggtcgataaa aatgtgcagg ccaacggtac ggaaaaagcc gcaaaagcct acctgaactg
    20521 gctctacagc ccacaggcgc agaccatcat caccgactat tactatcgtg tgaataaccc
    20581 ggaagtcatg gacaaactga aaaacaaatt cccgcagacc gagctgttcc gcgtggaaga
    20641 caaatttggc tcctggccgg aagtgatgaa aacccacttc accagcggcg gcgagttaga
    20701 caagctgtta gcggcggggc gtaattaatg tttgcagttt catcaagacg cgtgctgccg
    20761 ggctttacct taagcctcgg caccagtctg ctgtttgtgt gcctgatttt gctgctgccg
    20821 ctctccgcgc tggtgatgca gctggctcag atgagctggg cgcagtactg ggaggtgatc
    20881 accaacccgc aggtagtagc ggcctacaaa gtaacgctgc tgtcagcgtt tgtggcatcg
    20941 atttttaacg gcgtttttgg cctgctgatg gcgtggatct taacccgcta tcgctttccg
    21001 ggccgcacgc tgcttgatgc gctgatggat ttaccctttg cgctgccaac ggcagtcgcc
    21061 ggtttaacgc tagcctcgct cttttctgta aacggttttt acggtgaatg gctggcgaag
    21121 tttgatatca aagtcaccta tacctggctg gggattgcgg tggcgatggc ctttaccagc
    21181 attccgtttg tggtacgtac cgtgcagccg gtgctggaag agttaggccc ggaatatgaa
    21241 gaagcggcgg aaacgcttgg tgcaacgcgc tggcagagtt tctgcaaagt ggtgctgccg
    21301 gagctttctc cggcgctggt ggcgggcgtg gcgctgtcgt ttacccgtag tcttggtgaa
    21361 tttggcgcgg tgatttttat cgccggaaat atcgcgtgga agacggaagt gacgtcgctg
    21421 atgatttttg tgcgcttaca ggagtttgat tacccggcag cgagcgcgat tgcttcggtg
    21481 atcctcgcgg catctctgct gctgctgttc tcaattaaca ctctgcaaag tcgctttggt
    21541 cggcgtgtgg taggtcatta atggcggaag ttacccaatt gaagcgttat gacgcgcgcc
    21601 cgattaactg gggcaaatgg tttctgattg gcatcgggat gctggtttcg gcgttcatcc
    21661 tgctggtgcc gatgatttac atcttcgtgc aggcattcag caaggggctg atgccggttt
    21721 tacagaatct ggccgatccg gacatgctgc acgccatctg gctgacggtg atgatcgcgc
    21781 tgattgccgt accggtaaac ctggtgttcg gcattctgct ggcctggctg gtgacgcgct
    21841 ttaacttccc tggacgccag ttactgctga cgctactgga cattccgttt gccgtatcgc
    21901 cggtggttgc cggtctggtg tatctgctgt tctacggctc taacggcccg ctgggcggct
    21961 ggctggatga acataatctg caaattatgt tctcctggcc cggaatggtg ctggtcacca
    22021 tctttgtgac gtgtccgttt gtggtgcgcg agctggtgcc ggtgatgtta agccagggca
    22081 gccaggagga tgaagcggcg attttgcttg gcgcgtctgg ctggcagatg ttccgtcgcg
    22141 tcacattgcc gaatattcgc tgggcgctgc tttatggcgt ggtactgacc aacgcccgcg
    22201 cgattggcga gtttggcgca gtgtcggtgg tttccggctc gattcgcggc gaaacacttt
    22261 cgctgccgtt acagattgaa ttgctggagc aggactacaa caccgtcggc tcctttaccg
    22321 ctgcggcact gttaacgctg atggcgatta tcaccctgtt tttaaaaagt atgttgcagt
    22381 ggcgcctgga gaatcaggaa aaacgcgcac agcaggagga acatcatgag cattgagatt
    22441 gccaatatta agaagtcgtt tggtcgcacc caggtgctga acgatatctc actggatatt
    22501 ccttcaggtc agatggtcgc gttgctgggg ccgtccggtt ccgggaaaac cacgctgctg
    22561 cgcattatcg ccgggctgga gcatcaaacc agcgggcata ttcgcttcca cggcaccgac
    22621 gtcagccgcc tgcacgcgcg cgatcgcaag gtcggtttcg tgttccagca ttacgcgctg
    22681 ttccgccata tgacggtatt cgacaatatc gcttttggcc tgacggtgct gccgcgtcgc
    22741 gagcgcccga atgccgcagc catcaaagcg aaagtgacaa aattgctgga aatggtccag
    22801 cttgcgcatc tggcggatcg ttatccggcg cagctttccg gcggccagaa acagcgtgtg
    22861 gcgctggcgc gcgcgctggc tgtagaaccg caaattctgc tgcttgatga accgtttggc
    22921 gcgctggatg cgcaggtgcg taaagagctg cgtcgctggc tgcgtcaact gcatgaagag
    22981 ctgaaattca ccagcgtgtt tgtgacccac gaccaggaag aggcgaccga agtagccgat
    23041 cgtgtagtgg tgatgagtca ggggaatatt gaacaggctg atgcgccgaa tcaggtatgg
    23101 cgcgaaccag cgacccgttt tgtactcgaa tttatgggcg aagtgaaccg cctgcaggga
    23161 accattcgcg gcgggcagtt ccatgttggc gcacatcgct ggccgctggg ctacacacct
    23221 gcttatcagg ggccggtgga tctcttcctg cgcccgtggg aagtggatat cagccgccgt
    23281 accagtctcg attcgccgct gccggtacag gtactggaag ccagcccgaa aggtcactac
    23341 acccaattag tggtgcagcc gctggggtgg tacaacgaac cgctgacggt cgtgatgcat
    23401 ggcgacgatg ccccgcagcg tggcgagcgt ttattcgttg gtctgcaaca tgcgcggctg
    23461 tataacggcg acgagcgtat cgaaacccgc gatgaggaac ttgctctcgc acaaagcgcc
    23521 tgataggttg agtgaatgtt aaacgcccgg aggcgcttcc cgcgatccgg gctttttaat
    23581 ggcaaggttt gtaacctgta gacctgataa gacgcgcaag cgtcgcatca ggcaacacca
    23641 cgtatggata gagatcgtga gtacattaga acaaacaata ggcaatacgc ctctggtgaa
    23701 gttgcagcga atggggccgg ataacggcag tgaagtgtgg ttaaaactgg aaggcaataa
    23761 cccggcaggt tcggtgaaag atcgtgcggc actttcgatg atcgtcgagg cggaaaagcg
    23821 cggggaaatt aaaccgggtg atgtcttaat cgaagccacc agtggtaaca ccggcattgc
    23881 gctggcaatg attgccgcgc tgaaaggcta tcgcatgaaa ttgctgatgc ccgacaacat
    23941 gagccaggaa cgccgtgcgg cgatgcgtgc ttatggtgcg gaactgattc ttgtcaccaa
    24001 agagcagggc atggaaggtg cgcgcgatct ggcgctggag atggcgaatc gtggcgaagg
    24061 aaagctgctc gatcagttta ataatcccga taacccttac gcccattaca ccactaccgg
    24121 gccggaaatc tggcagcaaa ccggcgggcg cattacccat tttgtctcca gcatgggaac
    24181 aaccggcact atcaccggcg tttcacgctt tatgcgcgaa caatccaaac cagtaacgat
    24241 tgttggcctg cagccggaag aaggcagcag tattcccggc attcgccgct ggcctgcgga
    24301 atatctgccg gggattttca acgcttctct ggtggatgag gtgctggata ttcatcagcg
    24361 cgatgcagaa aacaccatgc gcgaactggc agtgcgggaa ggaatattct gtggcgtcag
    24421 ctccggcggc gcggttgccg gagcactgcg ggtggcaaaa gctaaccctg gtgcggtggt
    24481 ggtagcgatc atctgcgatc gtggcgatcg ctacctttcc accggggtgt ttggggaaga
    24541 gcattttagc cagggggcgg ggatttaaga ttattgcgca ggcaccgttt tatccgtctg
    24601 cgcaattagc gcatgtaaaa aaggctgtgc gttctcatcg ctcttaccgg ggatcttcac
    24661 aatatacggc ttcccggtaa tcgatgacga cgaagcaacc ttatcaataa actgctcggc
    24721 agtgtcaatg cggttacggg tattgcccag cttcagacgc agatgagaaa ccgcttcatc
    24781 gcaggtatgt tcatcaccgt tgcgcacaaa tatcaaatcc tttttctgtg ctaatccctc
    24841 cagcatggcg ttgatgcggg cttcttcatg ggcagttaac ttcgcgtaag cggggagtgt
    24901 cattagtagt gtaatgacca gacaaatgat tttcttcatt gaattacctt atccttgttt
    24961 tgtgatagcc gatttgactg ccagaatcag gtaaagtttc ggttggaata cgatgaggat
    25021 catcggcaag gagaaaaaat taaatgagta gtttgttgtt gtttaacgat aagagcaggg
    25081 cgctgcaggc ggatatcgtc gccgtgcagt cgcaggtggt ttacggcagc gtgggcaaca
    25141 gcattgccgt gcctgctatc aaacagaacg gtctgaatgt ctttgccgtg ccgacggtat
    25201 tgctgagcaa tacgccgcat tatgacactt tctacggtgg tgcgattccg gacgaatggt
    25261 ttagcggcta tttgcgtgcg cttcaggagc gtgatgcgct gcgccaactt cgtgctgtaa
    25321 ccacgggcta tatgggaacg gcatcgcaaa tcaaaatcct tgccgagtgg ctgactgcgc
    25381 tacgcaaaga ccatcctgac ctattgatca tggtcgatcc ggtgattggc gatattgata
    25441 gcggaattta tgtcaaacct gaccttcccg aggcgtatcg acaatattta ctgccgctgg
    25501 cgcagggaat tacccccaat atctttgagc tggaaatcct gaccggtaaa aattgccgcg
    25561 atctcgacag tgccattgct gccgcaaaaa gtctgctttc agacacatta aaatgggtgg
    25621 tgattaccag cgcctccggt aatgaagaaa atcaggagat gcaggtggtg gtggtcagtg
    25681 ccgacagcgt gaatgtcatt tcccattcac gggtaaaaac cgacctgaaa gggactggcg
    25741 acctgttttg tgctcagctt atcagtggtt tgctgaaagg gaaggcgtta aacgatgcgg
    25801 tgcaccgtgc ggggttgcgc gtactggaag tgatgcgcta cacccagcag catgagagcg
    25861 atgaattgat tttgccgccg ctggcggaag cataaaaaat ggcgccgatg ggcgccattt
    25921 ttcactgcgg caagaattac ttcttgatgc ggataaccgg ggtttcaccc acggttacgc
    25981 taccggacag tttgatcagt tctttgattt cgtccatgtt ggagataaca accggagtca
    26041 gggtagactt ggctttctct tccagcagcg gcagatcaaa ttcaatgaca gtatcgccaa
    26101 ctttcacgcg ctgaccttct tcagcaatac gcttgaagcc ttcgcctttc agttcaacgg
    26161 tgtcgatacc gaagtggacg aacagttcaa cgccgctatc agattcgata gagaacgcgt
    26221 ggttggtttc aaagatttta ccaatggtgc cgtctacagg cgcgaccatt ttgttacccg
    26281 ttggtttgat agcaatacca tcaccaacga ttttttccgc aaaaacgaca tccggcacgt
    26341 cttcgatatt gacgatctcg ccagagagcg gagcaatgat ctcaatagtt ccggtatcct
    26401 tcttgtcgtc ggaaaccaga gatttcagtt tatcgaacaa acccatgatc ttctcctaag
    26461 cagtaaattg ggccgcatct cgtggattag cagattgttt tttcttcaat gaacttgtta
    26521 accagcgtca ttaactcgtc cgttgtcggt tgagcaagag cctgctctgc taacaccttc
    26581 gcatcttcga agttcgtgtt acggataatc ttcttaatgc gcgggataga aatggcgctc
    26641 atagagaatt cgtccagacc catccccagc aacagaagtg tagcacgttc atcgccagca
    26701 agctcaccac acatgccagt ccatttgcct tcagcatgag aagcatcaat aacttgcttg
    26761 atcaagttca gcacggacgg tgacattggt tggtaaaggt gtgaaatcat atcattacca
    26821 cggtcaactg ccagagtgta ctgcgttaaa tcattggtgc cgatactaaa gaaatcaact
    26881 tctttggcta aatgacgtgc aattgttgcg gcagccggtg tttccaccat tacgccgatt
    26941 tcaattgact cgtcaaacgc tttaccttcg tcgcgcagtt cctgtttgta gatttcgatc
    27001 tctttgcgca gtgcacgcac ttcttcaaca gagatgatca tcgggaacat aatgcgcaat
    27061 ttaccgaaag ccgaggcacg caggatagcg cggagctgat cgcgcaggat ctctttacga
    27121 tccatcgcga tacggatagc gcgccagccg aggaacgggt tctcttcttt cgggaagttc
    27181 atgtatggca gctctttgtc gccgccgatg tccatggtac gaacgataac cgcctgcgag
    27241 ccacacgctt cagccactgc tttgtaagca gcaaactgtt cttcttcagt gggcagcgcg
    27301 tcgcggtcca tgaacaggaa ctcagtacga tacagaccaa cgccttcagc gccgttacgc
    27361 tctgcacctt caacgtcacg aaccgtacca atgttagcgc acacttctac ctggtgaccg
    27421 tccagcgtaa tagctggcag atctttcagt ttagcaagct ctgctttttc agaagccact
    27481 tgctcctgaa cagcgcgcat tttatcaata acttcgttgg ttggattgac gtaaacctga
    27541 ttatttacgg catccagaat cagatagtcg tcatttttca cctgagaggt gacgctaccg
    27601 gtacccacga tagcaggcag ttccagagaa cgcgccatga tagaggtgtg ggaagtacgg
    27661 ccacccgcgt cggtgatgaa acccagcacc ttcttcaggt tcagctgtgc ggtttcggac
    27721 ggcgtcaggt cagcggcaac cagaatgact tcatcctgaa tggcgctcag gtcgataatc
    27781 ttcaggccca ggatgttgcg cagcaggcgc ttaccgatat cacgtacgtc agccgcacgt
    27841 tctttcaggt attcatcatc cagctcttcc agggcagaag cctgaccttc gataacttca
    27901 tgagcagctg cgtcagctgt catgtgctta tctttaatca gggctatgat ttcctgctcc
    27961 agctcctcat cttcgagcag cataatatgc ccttcaaaga tggcttcttt ttcttcaccg
    28021 aacgtttcac cagctttcgt tttgatcgtt tccagctggg ctgatgcctt ggcacgaccg
    28081 ctcagaaaac gttcaacttc ctgatcaacc tggtcggcag aaattttttt ccggtcaatg
    28141 acaatttcgt cttctttcag aagcagagct ttaccgaaag cgatacccgg ggatgctaaa
    28201 atgcctgaaa tcataaccct accttacttg tgactgattt ttaaaagaac ccgggaaatt
    28261 actcgagttc cgccatcagt ttaaccagat gttcaaccgc tttctgctcg tcttcgcctt
    28321 ctgcggagat agtcacaacg gtaccttgag tcaggcccag agtctgcagt ttaaacaggc
    28381 ttttcgcgct ggcgcttttg ccgttggaag tcacagtaat ttcagaagtg aagcccttag
    28441 cttcttttac aaactgggca gcagggcggg tgtgcagacc gttcggagcg gtaatggtaa
    28501 cttcttgctg gaacattgta tttccccaac ttataggttt agtgttgtgg aactaaagtc
    28561 tagcctggcg gttcgacttt agcctgtatt gttagcgcca gtttttaaca gacgcgacgc
    28621 acgaagagcg caatacagtc cattgctggc gggtgttgac cgctgacgtt tcgtacgtca
    28681 ttaaacatta tgccgctaaa ggaagaattg aaccaaatca taaaatcgat tcagctttgg
    28741 cttttcctgt aacgattaat ttcgcgcatc aaaataatta gtctggttaa ataccagttc
    28801 cgacaggctg aatcaatgcc aggaggggcg aaagtttgaa gcaggccaca aaaaagcacc
    28861 taaaaaggtg cttttttacg catttttaac aagctggcat tactgttgca gttctttctc
    28921 agtgaagaga tcggcaaaca atgcggtgct taaataacgc tcacccgatg acggtagaat
    28981 aaccacaata ttcttgttgg taaagctttc atcttcttgt agtttcaacg ccgcagcaac
    29041 agctgctcca gaagagatac ctgcaagaat accttcttct tccatcagac gacgtgcggt
    29101 agaaatcgct tcttcattgg tgatgccaat gactttatcg accagcttga gatcgaggtt
    29161 agctgggata aaaccagcgc caataccctg aattttatgc gggccaggtt taatctcttc
    29221 accagccagt gcctgggcga taactggaga atcggttggc tcaacggcga cagagataag
    29281 atcggtcttg cctttggtgc ctttaatgta gcggctgacg ccagtcagcg taccgccagt
    29341 cccaacgcca gcaataaata catcaacctg accgtcggta tcttcccaga tctccggacc
    29401 ggtggttttt tcgtgaattt cagggtttgc cggattgctg aattgttgca gcagcaggta
    29461 tttctctgga ttgctggcga caatttcttc tgctttttgg attgcgcctt tcatgccttt
    29521 agcaccttcc gtcagcacca ggtttgcacc taacgctttc agcagcttgc ggcgttcaat
    29581 actcatggtt tctggcatgg tcagggtgag tttgtaaccg cgagcggcag ctacataggc
    29641 cagtgcaatc ccggtattac cgctggtcgg ttcaaccagt tcaacgcctg gtttcagcac
    29701 gccgcgcttt tcggcatccc aaatcatgtt ggcaccgata cggcacttaa cgctgaagct
    29761 ggggttacga gattccacct tcgccagaat gcgtccgtta ccgatgcgat tcaggcgaac
    29821 cagcggcgtg tgaccgatag tcagcgagtt atcttcaaaa atcttactca tggcctgtcc
    29881 ttaactgtat gaaattggga tacaacaggt agcatacccg ctcagagaat atgcggaagt
    29941 aaggatttag catatctata tacagaaggg aaataatgac atgcaagatg gaataagggg
    30001 cggcataagc cacccctgtt tcacacaaac ggtttacaaa ataaccggta gattgttacc
    30061 gccacatcgc gtgtttatcg cgatagcaat cgacccacat cgccgtcgcg ccacaaacgg
    30121 caacgggcat gatgaacaga ttaagcagcg ggatcatcgt aaacaggctg gttaaagcac
    30181 caaactgcat attggtgatt ttgcgtgtgc gcagggcggt gcgcatctct ttaaacggca
    30241 ctttgtggtt atcgaagggg taatcgcaat actggatggc taacatccag gcgctaaaca
    30301 ggaaccacag taccggcgcg acggtttgcc caataccagg gattaagtaa agaattagca
    30361 atacaattgc gcgcggcaga taccaggcaa atttttgcca ttcgcgtttc atgattcgcg
    30421 gcacatcttt catgataccg aaaatcccgg tatctggcgg tgtagcgcca gtcaatcgtg
    30481 cttccagttg ttcagccaat aaaccgttaa acggagcggc aatccagtta gcaatcgtgg
    30541 agaagaaata gccaaacact aacagcacag agatgacagc cagaggccac aacagataac
    30601 tcagccattg tagccagtcc ggaacgtaac tcatgagagt cgggatccag acatcgagct
    30661 gtgtaaagag ccaccagaat gcgcccccca tcaacaaaat attgaccagc agcggtaaaa
    30721 taacgaaacg ccgaatccca ggttgcgaga cgagcttcca gccttgcgca aaatagtaaa
    30781 aaccgctgcg tggggcagat gtgaatgatg aaaccataat caggatgagc tccttttgac
    30841 caatcccagg aaaattctgc gtattttacc gggtaattgc gcaatggaca gttaggatat
    30901 gttcgaaaaa acagcaaaaa gcacgatttc atctatcttt gtgctgtgaa agttaatagt
    30961 gcacttgcac ttgaggtaat cggcaaatac tcttagtgag taaatgtttg ccgtggtggc
    31021 aaggtgttag aacaacagag aatataatga tgcaggattt gcgtctgata ttaatcattg
    31081 ttggcgcgat cgccataatc gctttactgg tacatggttt ctggaccagc cgtaaagaac
    31141 gatcttctat gttccgcgat cggccattaa aacgaatgaa gtcaaaacgt gacgacgatt
    31201 cttatgacga ggatgtcgaa gatgatgagg gcgttggtga ggttcgtgtt caccgcgtga
    31261 atcatgcccc ggctaacgct caggagcatg aggctgctcg tccgtcgccg caacaccagt
    31321 accaaccgcc ttatgcgtct gcgcagccgc gtcaaccggt ccagcagccg cctgaagcgc
    31381 aggtaccgcc gcaacatgct ccgcgtccag cgcagccggt gcagcagcct gcctatcagc
    31441 cgcagcctga acagccgttg cagcagccag tttcgccaca ggtcgcgcca gcgccgcagc
    31501 ctgtgcattc agcaccgcaa ccggcacaac aggctttcca gcctgcagaa cccgtagcgg
    31561 caccacagcc tgagcctgta gcggaaccgg ctccagttat ggataaaccg aagcgcaaag
    31621 aagcggtgat tatcatgaac gtcgcggcgc atcacggtag cgagctaaac ggtgaactgc
    31681 ttcttaacag cattcaacaa gcgggcttca tttttggcga tatgaatatt taccatcgtc
    31741 atcttagccc ggatggtagc ggcccggcgt tattcagcct ggcgaatatg gtgaaaccgg
    31801 gaacctttga tcctgaaatg aaggatttca ctactccggg tgtcaccatc tttatgcagg
    31861 taccgtctta cggtgacgag ctgcagaact tcaagctgat gctgcaatct gcgcagcata
    31921 ttgccgatga agtgggcggt gtcgtgcttg acgatcagcg ccgtatgatg actccgcaga
    31981 aattgcgcga gtaccaggac atcatccgcg aagtcaaaga cgccaacgcc tgatacactt
    32041 aaggcaaatt aactcctctt cgaacccccg cttgtcgggg gtttttagca ttgatggtgc
    32101 gatatggaat caatcgaaca acaactgaca gaactgcgaa cgacgcttcg ccatcatgaa
    32161 tatctttatc atgtgatgga tgcgccggaa attcccgacg ctgaatacga caggctgatg
    32221 cgcgaactgc gcgagctgga aaccaaacat ccagaactga ttacgcctga ttcgcctacc
    32281 caacgtgtag gcgctgcgcc gctggcggct ttcagccaga tacgccatga agtaccaatg
    32341 ctgtcactgg ataacgtttt tgatgaagaa agctttcttg ctttcaacaa acgtgtgcag
    32401 gaccgtctga aaaacaacga gaaagtcacc tggtgctgtg agctgaagct ggatggcctt
    32461 gccgtcagta ttctgtatga aaatggcgtt ttagtcagtg ccgcgacccg tggcgatggc
    32521 accaccgggg aagatatcac gtctaatgtg cgtactattc gcgctattcc gctgaagctg
    32581 cacggagaga atatcccggc gcgtctggaa gtgcgtggtg aagtgttcct gccgcaggcg
    32641 gggttcgaaa agattaacga agatgcgcga cgcacgggcg ggaaagtttt tgctaaccca
    32701 cgtaatgcgg cagctggttc actgcgtcag cttgatccgc gtattacagc gaagcgaccg
    32761 ctcacttttt tctgctatgg cgttggtgtt ctggaaggtg gcgagctgcc ggatactcat
    32821 cttggccgtt tactgcaatt taaaaagtgg gggttgccgg tcagcgatcg ggtaacgctt
    32881 tgtgaatcgg cggaagaagt gctggcgttc tatcacaaag tggaagaaga ccgcccgacg
    32941 ctgggctttg atatcgacgg cgtggtgatt aaggtcaact cactggaaca gcaggagcag
    33001 cttggctttg tcgcgcgtgc cccgcgctgg gcggtagcgt ttaaattccc ggcgcaggag
    33061 cagatgacct ttgtgcgtga cgtcgagttt caggttgggc gtactggcgc gattacgcct
    33121 gttgcgcgtc tggaacctgt ccatgttgct ggcgtgctgg tgagtaacgc aaccttacac
    33181 aatgcggatg aaatcgaacg tcttggttta cgcattggcg ataaagtggt gattcgccgc
    33241 gctggcgacg tgatcccgca ggtggttaac gtcgtgcttt ctgaacgccc ggaagatacc
    33301 cgtgaggttg tattcccgac gcattgtccg gtatgtggtt ctgacgttga gcgtgtggaa
    33361 ggtgaagcgg ttgcccgctg taccggtggc ctgatttgcg gagcgcagcg taaagagtcg
    33421 ctgaaacact ttgtttcccg ccgtgcgatg gatgttgacg gaatgggcga caaaatcatc
    33481 gatcagctgg ttgaaaaaga atatgtccat actccggcag atctgttcaa actcaccgca
    33541 ggcaaactga ccggactgga gcgtatgggg ccaaaatcgg cacaaaacgt ggttaacgcg
    33601 ctagaaaaag cgaaagaaac cacctttgct cgcttcctct atgcacttgg catccgtgaa
    33661 gtcggcgagg ccaccgcagc aggtctggcg gcatatttcg gcacgctgga agcgctggaa
    33721 gccgcttcga ttgaagagct gcaaaaggtg cctgatgttg gcattgtcgt tgcatcccac
    33781 gttcacaact tctttgccga agaaagcaac cgcaatgtca tcagcgagct gttggcggaa
    33841 ggtgttcact ggcctgcgcc gatcgttatc aacgcggaag agattgacag cccgtttgct
    33901 ggtaaaaccg tggtgcttac gggcagctta agtcagatgt cacgtgatga cgctaaagct
    33961 cgactggtcg aactgggcgc gaaagtcgcg ggcagcgtgt cgaagaaaac cgatctggtg
    34021 atagcgggtg aagctgcagg atctaaactg gcgaaggcgc aggaactggg cattgaagtc
    34081 atcgacgaag cggaaatgct gcgtttgctg ggtagctgag atggaaaaag agcagctgat
    34141 tgaaatagcc aatacgataa tgccgtttgg caaatacaaa gggcgtcgct taatcgacct
    34201 gccggaggag tatctgctgt ggtttgcccg caaagatgag ttcccggcag ggaagctcgg
    34261 tgagctaatg caaatcacgc tgctgattaa aaccgagggg ctgacgcaac tggtccagcc
    34321 cctgaagcgt ccgctttaag ctttatcggt gctgctttcc tgctgcgcct gtaactgttc
    34381 ggtctggcgt ttgtatcgac gcgccagcac cgcacagacc atcaattgga tctgatggaa
    34441 aatcatcagg ggcagcacca tcataccgat caccgatgtg gggaacagaa tgtttgccat
    34501 cgggatgcca tttgccagac tctttttcga accacaaaag acgatagtaa tttcatctgc
    34561 cttattgaag cccagtcggc gtgccataaa gacgttaact acaatcacga tagccagaag
    34621 aacgcagctg accaccacga taaacagcaa tgatccccag ccaactttat gccagatacc
    34681 attaacgacg gcttcgctga acgctgtata aaccaccaac agaatggacg tctggtcagt
    34741 tttcgcaatc cattttttat tgcgcgacac ccagtcacca atccacggcc gggaaagatg
    34801 ccccaacaca aacggcagca gcagttgcag cataatttta ccgacctgct caaggctgcc
    34861 ccctgcaccg tgaacattca tcaccagacc aaccagcaat ggtgaaagga aaatccccag
    34921 caggctggat gctgacgcag aacaaaccgc cgccgcgacg ttaccgcccg ccattgacgt
    34981 gaaggcgatt gcagactgca cggtagccgg gagaatgcac aagtagagaa aaccggagta
    35041 gagcatcggg tcgacattta ccggtttcca ccaggcaaac agtacaccca gaatcggaaa
    35101 cagcacgaag gtgctgcaaa ttacccacaa atgcagtcgc cagtgaccac cgccagcaat
    35161 aatcgcctca cgcgacaact tcgcgccgtg cataaagaac agcagggcaa tcgctgcggt
    35221 ggtcagattt tcaaagaagg ggacgaaatc gcctctggcc ggaaagaaag aggccagcaa
    35281 caccaccgtg atcagggtta aggtgaaagg atcgaggata cgaaaaagtt tcataaagac
    35341 tccagatgat cgatggttct attgtgcttc ttccagtttg agaaataaaa ttgatttatt
    35401 gcatccattc atgaagaaaa cagatgaatt attctttaaa acaattaaag gttttcgtca
    35461 cagtagcgca ggagaaaagt tttagtcgtg caggagagcg tatcggcctg agccagtcgg
    35521 cagtgagtca cagtgtgaag gaactggaaa atcatactgg tgttcgcctg ctggacagaa
    35581 ccacgcgtga agtggtgctt acagatgcag ggcagcagct ggctttgcgt cttgagcgac
    35641 tgctggatga actgaacagc acgttgcgcg ataccgggcg tatggggcaa caactgagcg
    35701 gaaaagttcg ggtcgctgcc agccagacca tttccgcgca tcttattccg caatgcattg
    35761 ctgaaagcca tcgccgctat ccagatattc agtttgtcct gcacgatcgt ccgcagcagt
    35821 gggtgatgga aagtattcgc cagggagatg tcgactttgg catcgtcatc gatccaggcc
    35881 cagtgggcga tctgcaatgt gaagcgattc tttccgagcc tttctttctg ctttgccatc
    35941 gcgatagcgc tttggccgtc gaagattatg taccctggca ggcattacag ggagcgaaat
    36001 tggtgttgca ggattacgcg tcaggcagcc gaccgctgat tgacgcagcg ctggcgcgca
    36061 atggtattca ggcgaatatt gtgcaggaga ttggacatcc ggcgacgctg ttcccgatgg
    36121 tagccgcagg catcggcatc agtattcttc ccgcactggc gctaccattg cctgaaggta
    36181 gcccactggt ggtaaagcgc attacgccgg ttgttgaacg ccagttgatg ctggtgcgca
    36241 ggaagaaccg ttcactttcc accgccgctg aagcgttatg ggacgtggta cgagaccagg
    36301 gcaatgcgct aatggctggt cgggaagggg atccgttgta tcagatatag agtgaattca
    36361 tactgccggg agccccggca gtaatcaatc agaagttaaa tttgatggag tagattatcg
    36421 catcctggta aaaatcttcg cccagtttgt catccgcata tcgatactga atccccgtag
    36481 taatgtgtga tgttgcattc caccacagtg caactgcgcc attcagccct tctttaccgc
    36541 cattacccgc tgcataggta gcgtcacggt caaactcgta ctcgttccag ttggtaagag
    36601 tgaatttctc gctgcccagc ataaagttgt aacctgcaac ccagccggcg acatagccgt
    36661 tgtcgccagt gtaataggtt tgatctgtat aacgcttagc aaagaacggt ttgaaccacc
    36721 aaccgctgcc ggtgaaattg tagccgatac cgtacaggaa catatcgtcg tggaaattca
    36781 cgcgattcgc agaaccgtag gtgccatacg catgcaagta gagattaaat ccggtgtcac
    36841 ccaggtaaat acggttggta tttttaaagg tataacgctg ctcgctacct ggtttattat
    36901 ggcgaccgtt atagaagttt tcccagtcga agaacccgta catttctccc caactaaagt
    36961 tagcgccacc ttcaaattca agataaccaa aatcatcttt gtgtgatttg gtcgacgatt
    37021 tttcggtagt ccggctggtc cagtccagat aatgcacgcc gatatctgca aaaccgcctt
    37081 taaattctgc atgagataca acaggtatcg ctaatataga ggaaagtgtc agagttaaaa
    37141 gatgtttttt catagcataa ttccctatgc cgatcgttat tagtagattg cttaataaag
    37201 tggtttgcta aaaacgacat attgttaatg caaaggaatt aatatcgcca atacagtttt
    37261 taccgtggat taatagagaa aagatataaa tccatcgata tcgaatcgct attgaatgcg
    37321 tgagaaaagt gtattggtaa gagccgggac aacctcgcta taaaaaggcg ttagattcca
    37381 ccctacagaa aaaggatatg tatgtctcag gttcaatttt ctcataaccc gctgttttgc
    37441 atagatatta tcaagactta taaacctgat ttcacgccac gagtggcctt tattttaggt
    37501 tccgggctcg gcgcgctggc cgatcagatt gagaacgctg tcgcaatttc ctacgaaaag
    37561 ctgccgggct tcccggtaag tacggtacat ggtcatgcgg gggagttagt gctgggccat
    37621 ctccagggcg taccggtggt atgtatgaaa ggtcgcggac atttctacga aggtcgtgga
    37681 atgaccatta tgactgacgc aattcgtacc tttaagctgc tgggctgcga gctactgttc
    37741 tgcaccaatg cggcaggctc gctgcgtccg gaagtggggg caggcagcct ggtcgcattg
    37801 aaagatcata tcaacaccat gcccggtacg ccgatggtgg gtcttaacga tgatcgtttt
    37861 ggagagcgct tcttctcact ggcaaatgcc tacgatgcgg aataccgcgc actgttacaa
    37921 aaagtggcga aagaagaggg tttccctctg acggagggcg tgttcgtctc gtatccgggg
    37981 ccgaatttcg agactgcggc ggaaattcgc atgatgcaaa ttattggtgg ggatgttgtt
    38041 ggtatgtctg tggtgcctga ggttatttca gctcgccatt gcgaccttaa agtcgttgcg
    38101 gtctctgcga ttaccaatat ggcggaaggt ttaagcgatg tgaaattgtc acatgcgcaa
    38161 acgctggcgg ctgcggagct ctctaagcag aacttcatca accttatttg tggctttctg
    38221 cgcaaaattg cctgagaaaa acaataagaa acggcctcgc gggtgaggcc aaatgatgcg
    38281 acaaggaaac gattatgagc atcgcgatgc gcttaaaggt aatgtccttt ttgcaatatt
    38341 ttatctgggg gagctggctg gttaccctcg gctcttacat gattaatacc cttcatttca
    38401 ccggcgctaa tgttggcatg gtttacagtt ccaaagggat cgccgcgatt attatgcctg
    38461 gtataatggg gatcatcgca gacaaatggc tgcgcgcaga acgtgcatac atgctgtgtc
    38521 acctggtgtg tgcgggcgta cttttttatg cggcatccgt aactgatccg gatatgatgt
    38581 tttgggtgat gttagtcaat gcgatggcgt ttatgccgac tattgcgtta tcgaacagcg
    38641 tctcttattc ctgtcttgcc caggcagggc ttgacccggt gaccgctttc ccgcccattc
    38701 gcgtttttgg tacggtgggg ttcattgtcg cgatgtgggc agtaagcctg ctgcatctgg
    38761 aattgagtag tctgcagctg tatatcgcgt ccggtgcgtc attgctgctg tcggcttatg
    38821 cgctgacttt gccgaagatt ccggttgcgg agaaaaaagc gaccacatcg cttgccagca
    38881 agctgggtct ggatgccttc gtgctgttta aaaatccacg catggccatc tttttcctct
    38941 ttgccatgat gctgggtgcg gtactgcaaa ttaccaacgt ttttggtaat ccgttcctac
    39001 atgatttcgc ccgtaacccg gagtttgctg acagttttgt ggtgaaatat ccctccattt
    39061 tactgtcagt ttcacagatg gcagaagtgg gctttatact gactatccca ttctttttaa
    39121 agcgatttgg cattaaaacc gtcatgctga tgagtatggt ggcctggacg ctgcgctttg
    39181 gcttcttcgc ctatggcgat ccgtcaacaa ccggatttat tttgctgctg ctgtcgatga
    39241 ttgtttatgg ctgtgcattc gatttcttca atatttctgg ttcggtattt gtcgaacagg
    39301 aagttgattc cagcattcgt gccagcgcgc aggggctctt tatgaccatg gtaaatggtg
    39361 tcggcgcatg ggttggctcg attctgagtg gcatggcagt agattacttt tcggtggatg
    39421 gcgtaaaaga ctggcaaact atctggctgg tgtttgcagg atatgctctt tttctcgcag
    39481 tgatattttt ctttgggttt aaatataatc atgaccctga aaagataaag catcgagcgg
    39541 tggctcatta aaaggattcg cagctctgct cttcagagct gcttttatga taaaggttaa
    39601 ttagtgaaag atattttatt ttcttccggc gttggttttg gcattggtgc tttgtttaca
    39661 atcgtgcgct tgcctattcc tgtaccaaat gtattaccag gtatattatc cattgtgttt
    39721 atgtatgtcg gatatct
//
LOCUS       DAZFXT010000034        37967 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.034, whole genome shotgun sequence.
ACCESSION   DAZFXT010000034 DAZFXT010000000
VERSION     DAZFXT010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37967
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.034"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(112..399)
                     /gene="yebO"
                     /locus_tag="ACHKW0_001846"
     CDS             complement(112..399)
                     /gene="yebO"
                     /locus_tag="ACHKW0_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HFO7474691.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(474..617)
                     /gene="mgrB"
                     /locus_tag="ACHKW0_001847"
     CDS             complement(474..617)
                     /gene="mgrB"
                     /locus_tag="ACHKW0_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HFO7474692.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            776..1015
                     /gene="yobH"
                     /locus_tag="ACHKW0_001848"
     CDS             776..1015
                     /gene="yobH"
                     /locus_tag="ACHKW0_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobH family protein"
                     /protein_id="HFO7474693.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1158..1949)
                     /gene="kdgR"
                     /locus_tag="ACHKW0_001849"
     CDS             complement(1158..1949)
                     /gene="kdgR"
                     /locus_tag="ACHKW0_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HFO7474694.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2126..3499
                     /gene="yebQ"
                     /locus_tag="ACHKW0_001850"
     CDS             2126..3499
                     /gene="yebQ"
                     /locus_tag="ACHKW0_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474695.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3545..4426)
                     /gene="htpX"
                     /locus_tag="ACHKW0_001851"
     CDS             complement(3545..4426)
                     /gene="htpX"
                     /locus_tag="ACHKW0_001851"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HFO7474696.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4618..6666)
                     /gene="prc"
                     /locus_tag="ACHKW0_001852"
     CDS             complement(4618..6666)
                     /gene="prc"
                     /locus_tag="ACHKW0_001852"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HFO7474697.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6686..7384)
                     /gene="proQ"
                     /locus_tag="ACHKW0_001853"
     CDS             complement(6686..7384)
                     /gene="proQ"
                     /locus_tag="ACHKW0_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0033592 - RNA strand annealing activity
                     [Evidence IEA];
                     GO_function: GO:0034057 - RNA strand-exchange activity
                     [Evidence IEA];
                     GO_process: GO:0010608 - post-transcriptional regulation
                     of gene expression [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HFO7474698.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7481..7978)
                     /gene="msrC"
                     /locus_tag="ACHKW0_001854"
     CDS             complement(7481..7978)
                     /gene="msrC"
                     /locus_tag="ACHKW0_001854"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HFO7474699.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8108..9391
                     /gene="yebS"
                     /locus_tag="ACHKW0_001855"
     CDS             8108..9391
                     /gene="yebS"
                     /locus_tag="ACHKW0_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HFO7474700.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9360..11993
                     /gene="yebT"
                     /locus_tag="ACHKW0_001856"
     CDS             9360..11993
                     /gene="yebT"
                     /locus_tag="ACHKW0_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HFO7474701.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISATGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12073..13512
                     /gene="rsmF"
                     /locus_tag="ACHKW0_001857"
     CDS             12073..13512
                     /gene="rsmF"
                     /locus_tag="ACHKW0_001857"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HFO7474702.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADDNAPQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAASVGLNWDGNLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13630..13866
                     /gene="yebV"
                     /locus_tag="ACHKW0_001858"
     CDS             13630..13866
                     /gene="yebV"
                     /locus_tag="ACHKW0_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebV family protein"
                     /protein_id="HFO7474703.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13971..14162
                     /gene="yebW"
                     /locus_tag="ACHKW0_001859"
     CDS             13971..14162
                     /gene="yebW"
                     /locus_tag="ACHKW0_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HFO7474704.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14163..14819)
                     /gene="pphA"
                     /locus_tag="ACHKW0_001860"
     CDS             complement(14163..14819)
                     /gene="pphA"
                     /locus_tag="ACHKW0_001860"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HFO7474705.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCGFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15215..15556)
                     /gene="yebY"
                     /locus_tag="ACHKW0_001861"
     CDS             complement(15215..15556)
                     /gene="yebY"
                     /locus_tag="ACHKW0_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HFO7474706.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15569..16441)
                     /gene="copD"
                     /locus_tag="ACHKW0_001862"
     CDS             complement(15569..16441)
                     /gene="copD"
                     /locus_tag="ACHKW0_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HFO7474707.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGIGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16445..16819)
                     /gene="yobA"
                     /locus_tag="ACHKW0_001863"
     CDS             complement(16445..16819)
                     /gene="yobA"
                     /locus_tag="ACHKW0_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HFO7474708.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16958..17188
                     /gene="holE"
                     /locus_tag="ACHKW0_001864"
     CDS             16958..17188
                     /gene="holE"
                     /locus_tag="ACHKW0_001864"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HFO7474709.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17290..17946
                     /gene="yobB"
                     /locus_tag="ACHKW0_001865"
     CDS             17290..17946
                     /gene="yobB"
                     /locus_tag="ACHKW0_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein YobB"
                     /protein_id="HFO7474710.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPQGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17970..18632
                     /gene="exoX"
                     /locus_tag="ACHKW0_001866"
     CDS             17970..18632
                     /gene="exoX"
                     /locus_tag="ACHKW0_001866"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HFO7474711.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18629..20689)
                     /gene="ptrB"
                     /locus_tag="ACHKW0_001867"
     CDS             complement(18629..20689)
                     /gene="ptrB"
                     /locus_tag="ACHKW0_001867"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HFO7474712.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRLRDEQQWEELIPPRDNIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20898..21557)
                     /gene="yebE"
                     /locus_tag="ACHKW0_001868"
     CDS             complement(20898..21557)
                     /gene="yebE"
                     /locus_tag="ACHKW0_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7474713.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21884..22240)
                     /gene="yebF"
                     /locus_tag="ACHKW0_001869"
     CDS             complement(21884..22240)
                     /gene="yebF"
                     /locus_tag="ACHKW0_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HFO7474714.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22307..22597)
                     /gene="yebG"
                     /locus_tag="ACHKW0_001870"
     CDS             complement(22307..22597)
                     /gene="yebG"
                     /locus_tag="ACHKW0_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HFO7474715.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22731..23909
                     /gene="purT"
                     /locus_tag="ACHKW0_001871"
     CDS             22731..23909
                     /gene="purT"
                     /locus_tag="ACHKW0_001871"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HFO7474716.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23965..24606)
                     /gene="kdgA"
                     /locus_tag="ACHKW0_001872"
                     /gene_synonym="eda"
     CDS             complement(23965..24606)
                     /gene="kdgA"
                     /locus_tag="ACHKW0_001872"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HFO7474717.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24643..26454)
                     /gene="edd"
                     /locus_tag="ACHKW0_001873"
     CDS             complement(24643..26454)
                     /gene="edd"
                     /locus_tag="ACHKW0_001873"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004456 - phosphogluconate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009255 - Entner-Doudoroff pathway through
                     6-phosphogluconate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HFO7474718.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26689..28164)
                     /gene="zwf"
                     /locus_tag="ACHKW0_001874"
     CDS             complement(26689..28164)
                     /gene="zwf"
                     /locus_tag="ACHKW0_001874"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HFO7474719.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     YNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28502..29371
                     /gene="hexR"
                     /locus_tag="ACHKW0_001875"
                     /gene_synonym="yebK"
     CDS             28502..29371
                     /gene="hexR"
                     /locus_tag="ACHKW0_001875"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HFO7474720.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29499..30941
                     /gene="pyk"
                     /locus_tag="ACHKW0_001876"
     CDS             29499..30941
                     /gene="pyk"
                     /locus_tag="ACHKW0_001876"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HFO7474721.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31072..32043)
                     /gene="lpxM"
                     /locus_tag="ACHKW0_001877"
                     /gene_synonym="msbB"
     CDS             complement(31072..32043)
                     /gene="lpxM"
                     /locus_tag="ACHKW0_001877"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7474722.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32163..33485)
                     /gene="mepM"
                     /locus_tag="ACHKW0_001878"
     CDS             complement(32163..33485)
                     /gene="mepM"
                     /locus_tag="ACHKW0_001878"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HFO7474723.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33501..34433)
                     /gene="znuA"
                     /locus_tag="ACHKW0_001879"
     CDS             complement(33501..34433)
                     /gene="znuA"
                     /locus_tag="ACHKW0_001879"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HFO7474724.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34512..35267
                     /gene="znuC"
                     /locus_tag="ACHKW0_001880"
     CDS             34512..35267
                     /gene="znuC"
                     /locus_tag="ACHKW0_001880"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015633 - ABC-type zinc transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HFO7474725.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35264..36049
                     /gene="znuB"
                     /locus_tag="ACHKW0_001881"
     CDS             35264..36049
                     /gene="znuB"
                     /locus_tag="ACHKW0_001881"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HFO7474726.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36196..37206)
                     /gene="ruvB"
                     /locus_tag="ACHKW0_001882"
     CDS             complement(36196..37206)
                     /gene="ruvB"
                     /locus_tag="ACHKW0_001882"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HFO7474727.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37215..37826)
                     /gene="ruvA"
                     /locus_tag="ACHKW0_001883"
     CDS             complement(37215..37826)
                     /gene="ruvA"
                     /locus_tag="ACHKW0_001883"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HFO7474728.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9101 a         9805 c         9357 g         9704 t
ORIGIN      
        1 ttgaagtcag accagagttt taatttgaag cagttgcacc atggcagtgc gtataatcag
       61 gatgaattga gtaacccttc aacgacgggg ttactcaaga ttgaggaagg attatcgttc
      121 ggcgaccaga cgaataatat cttcgtcttc aaccgatttt tgactctcaa cggtcttttt
      181 atctgctttc tcaggttcgt ttgcttcgca caaacgacgt aacagtgcat tttgacgttt
      241 ttgctgatcc agcaacgcct caagcagttc aatctgttcg ttagtccgag aactggcacg
      301 attaataaaa aaccacaaga tgagcccgat aagaagaacc accaccgata caaccaaaga
      361 cgcaatgttc atcacgcctg aatttacaac ttcgttcatt tcaccacctc tgagtagagg
      421 cgctattcta ccactgctgg agaggaagaa aatctagtgc tgaaaaaatg atatcaccac
      481 gggataaact ggttaatggc acaaattccg ctgaaaaatt gtacatcctg atcgcacatc
      541 atgttgaata cctgcgccca aagcagcaag caagccaaca ccacgacaac cagaacgacc
      601 catcgaaact ttttcactcc aatctccgtt tcacctacct tatgtcatta aactagcatg
      661 cgttagttaa acagcgccta actatgtcga tattcgtgct ttttcggaat gagtcgcttg
      721 cttcagcgca tattgccgct acgattaagc gaacataaaa agagaagagg ttgtaatgcg
      781 attcatcatt cgcacagtta tgctgatagc actggtatgg ataggattat tactcagcgg
      841 atatggcgtt ttgattggaa gcaaagagaa tgccgcagga ttaggtttgc aatgtaccta
      901 tctgactgcc agaggcacca gcacggtaca atacttgcac actaaaagtg gtttcttggg
      961 gataacagat tgcccactat tacgtaaaag caatatcgtg gtcgataatg gttaagggta
     1021 ataaataacc agattctcac tcatgagtaa aatattctta tagcaagcgc gtttaatata
     1081 acgcaccttc aactttattt ttctcatgct aaataaagaa cctgtctgtt tgatattccg
     1141 acaggttctt actctcatca gaacggatag tcgtgataac ccatttgggc agaaattttg
     1201 cgcgctgcgg tatgcaacat tgcgacatat tcctgtaaac gctcttcaga gaaacgcaac
     1261 gtcgggaagg agatgctcaa accggcaatg accacgccaa agcgatcaaa taccggtacc
     1321 gcaatgcatc gcagcccttc ttcctgctct tcgttatctt cgccataccc ctgctcgcgc
     1381 acctggtcca gaacgggtaa taacgcttct gtactggtga tggtccgctc ggtactgcgt
     1441 ttatactcca cgccctcaag aatttgcttc acttcatcga gatcgcgcca tgccagcagt
     1501 accttaccaa tcgcggtgct gtacagcgga ttacgacgcc caatccgtga atacatgcgc
     1561 aaattgtaca tagagtcaat tttgtgtatg taaacaatac tgtcttcgtc cagtgcgccg
     1621 aggtggatag tttctttggt caggcgggag atctcacgca tctggatatc tgcgctacga
     1681 attaaatcga cgttttgtaa cgcgcgagcg cccagttcaa acaatttcag ggtcagggaa
     1741 tatttctccg actccccttc ctgcgccaca taacctaagg ttttcatggt ctgtaaaaag
     1801 cgataaacgg tgctttttga catcatgacg cgctgcgaca gctcggttat ccctatttcg
     1861 cgctcttcac ccagcgcctg caaaatgcca aaaactttta gcacggaaga tacagaatca
     1921 ggctgtttat ccagatctgc gttagccatt tatcacctca ttgcgagtgt tttataaaaa
     1981 tcagaactgt tttttattat aatttcgcac cagggtggtc gcaatccatc ttttgccggt
     2041 tagttacaat tctgcgacat ccaccgtgaa tatcagtgct agaatcatac ccctgttgat
     2101 tattcaccaa agatataaaa ttcctatgcc aaaagttcag gccgacggcc tgccattgcc
     2161 ccagcgatac ggtgcgatat taaccattgt gattggcatt tcgatggctg tccttgacgg
     2221 cgcaatcgcc aacgtcgccc tgccaacaat cgccacggac cttcatgcca cgccagccag
     2281 ttccatctgg gtagtgaacg cctatcaaat cgccattgtc atctccctgc tctcgttttc
     2341 gtttctgggc gatatgtttg gctatcgacg tatttataaa tgcggtctgg tcgtttttct
     2401 gttgtcttca ctgttctgcg ccctttctga ttcgctgcaa atgctcaccc ttgcgcgtgt
     2461 catacaaggt ttcggcggtg cagcgttgat gagcgttaat accgcactta tccgcctgat
     2521 ctatccacaa cgttttctgg ggagagggat gggcataaac tcgtttattg ttgccgtctc
     2581 ttctgctgcc gggccgacaa ttgctgcagc aatcctctcc atcgcatcct ggaaatggtt
     2641 atttttaatc aacgtaccgt taggtattat cgccctgctt ctggcgatgc gttttctgcc
     2701 acccaatggt tctcgcgcca gtaaaccccg tttcgacctg cccagcgccg tgatgaacgc
     2761 gttaaccttc ggcctgctta tcactgcgtt gagtggtttc gctcaggggc aatcgctgac
     2821 gttaattgct gcggaactgg tggtaatggt tgttgttggt attttcttta ttcgccgcca
     2881 gctttctctt cccgtaccgc tgctaccggt ggatttactg cgtatcccgc tgttttcact
     2941 ttctatttgc acatctgttt gctctttctg cgcacaaatg ctggcaatgg tttccctgcc
     3001 cttttacctg caaaccgtgc tcgggcgtag tgaagtcgaa acaggtttac ttctgacacc
     3061 gtggccgtta gcaacgatgg tgatggctcc gctggcaggc tatttgattg aacgcgtaca
     3121 tgcaggattg ctgggtgctt tagggttgtt catcatggct gcggggcttt tttccctggt
     3181 tctgctgccc gcgtcacctg cggatatcaa tattatctgg ccgatgatct tatgtggcgc
     3241 tggatttggc ttattccagt cacccaataa ccacaccatt attacctccg ctccgcgcga
     3301 acgtagcggt ggggccagtg gcatgttagg aacggctcgt ctactgggtc agagtagtgg
     3361 cgcggcgctg gtggcgctga tgctaaatca gttcggtgat aatggtacgc acgtttcgct
     3421 gatggctgcg gctattctgg cagtgattgc tgcctgtgtg agtggtttac gtatcactca
     3481 gccacgatcc agggcataaa aaaagcgcgt cgatcaggac gcgcttttta gtatttactt
     3541 catattactt caggtattca cccgtacgca gagcttcaat tcgtttatcc agcggcgggt
     3601 gagtcatgaa caactcactg agcgatttcg acttaccgtt aatgcagaaa gccatcatgc
     3661 tggttgcttc ttgcggttca tagctggttt tcaggcgctg cagcgcggca atcattttct
     3721 cgcgaccaac cagttttgcc gaaccggcat cagcatggaa ttcacgatga cgcgagaacc
     3781 acatggtgat aatgctcgca agaataccaa acaccagttc cagaaccgtt gcaaccgcaa
     3841 agtagatcag cgggttgccg ttgctctctt caccttcatc acgatttccg cccataaaac
     3901 ccgcggcaag ctgcgccaga atacgggaaa taaagataac gaaggtgttc accacgccct
     3961 gaatcagcgt catggtgacc atatcaccat tggcgatgtg gctgatttcg tgagcaatta
     4021 ccgcctcggc ttcatccggg ctcatgttct gcagcaaacc ggtgctgaca gcaaccagag
     4081 aggcatcacg gcgcgcaccg gttgcaaaag cgttgatgtc cggcgcatgg tagatagcca
     4141 cttgcggcat agcgatccct gcctgacgag cctgggttgc tacagtatta accagccaac
     4201 gttccctttc gttacgcggt tgctcgatca cttccccgcc aacagatcgt aatgccatcc
     4261 atttggacat cagaagcgaa acgaaggaac caccaaaacc gaacagcaag gccatgatca
     4321 tcagcccctg aacgctgctc gactgtatcc ctgtcaggct cagtaccagc ccgaaaacga
     4381 ccattacggc caggttcgtt agcaggaaga gcgcgattcg catcataatt ttctttttac
     4441 ctcagtttaa caaaacgcaa tatgcgatac ccacatcgta tgggttacgc gactattttc
     4501 aagtctggat agtgcgtaag tcaccagaaa gacacaactt tacattttgt agcatctgat
     4561 ttacggcatc ttgccgctgt taaaaaatca ggcacaattt cttgtgcctg attgatatta
     4621 cttgacggga gcgggttgtt ccgcgggtct ggctttttca agcttcgcca gatcgagtgc
     4681 gatattcacc gtctcatcca gataaggatc cggctcctgg taatctttag gtagatcatc
     4741 cagcttcttc aactccggtt taccttcgcg tttaaagcgt tcgttcaaac gcgccagacg
     4801 cgtcgcatca tcttcattat tctctttctc acgcacagcg tagttcagag aaacgatatt
     4861 gcgcttgtcc ttcatagcgt tgaagcgcgc gatatccttc atgatgttct ggaactcagg
     4921 atctttcgcg atacgcgcat tatgttcctt cagcagctcc ggcccaaagg ccgttaaatc
     4981 gcctgatttc acataagtcg cggcatcaat gctatcccac ggcagcgcgt tatcttcgaa
     5041 tttctcaccc gtttccgttt cttcattacc cgtcggcatg atgatgtctg gcgttacgcc
     5101 tttacgttgc gtactgccgc cgttaacgcg atagaatttc tggatcgtgt actgcacaga
     5161 acccagcgct ggccattcag gacgtaacat ctgatcgtaa atacggttca atgaacggta
     5221 ttgctgaacg gtgcctttac caaacgtcgg ttcacccaca accagcgcac gaccgtaatc
     5281 ctgcattgcc gcggcaaaga tttctgaagc cgaagcactg aaacggtcaa ccagtaccac
     5341 cagcgggcct ttatagaaca cctgcccgtc ggtatcgctg tcttcacgaa ccttgccgtt
     5401 gttatcgcgg acctgaacaa tgggacccgc aggaataaac agacctgaga gcgataccgc
     5461 ttcggtcagc gccccaccgc cattgctacg caggtcgatg atcacactgc tgacattctg
     5521 tttttccagt ttctgcagtt gaactttgac atcgtctgtc aaacccacat agaagcccgg
     5581 aatatccagc acgccgactt tctctttacc gacggtcttc accgacattt taaccgcgcg
     5641 gtcttcgaga cgaatacgtt cacgggtcaa tgttacagtg cgggtcttgg tccctttacc
     5701 agcaggtaaa atttccagac gaactttact gcccttcggc cctttaatta aggcaaccac
     5761 atcatcaaga cgccagccaa tcacgtcaac catcggcttg cctgtttgac caacaccgac
     5821 aattttgtca ccaacgctga tagctttact cttcgctgcc ggaccacctg ccaccatcga
     5881 attgataacg gtgtagtcat catccatttg cagcactgcg ccaatacctt ccagcgacaa
     5941 actcatttca gtgttgaact gttcggtatt acgcggggaa agatagttgg tatgcgggtc
     6001 gatttcacgc gcaaacgccg tcattgccag cgagaaaaca tcttcgctgt tggtttgcgc
     6061 cagacgacga atggcaaatt tgtagcggcg tgtcagggtt tcacgaattt ctttatccgt
     6121 ttttcctgcc agcttcaggc ttaactcgtc gaatttgact ttactgtccc acagcgcgtt
     6181 caactcagcc tcgtttttcg gccagggcgc tttgctgcgg tcaaggttat aagtgtcgtt
     6241 gccggtgaaa tccatcggct tttccagtac cgacaaagcg tactggtaac gctcaaaacg
     6301 gcgcttttgc gccagattgt agagatcgta gaaaacgtcg agtttgcctg aacgcagttc
     6361 atcgcctaac tcggttttct ttttcgcgaa ctgttcaaca tcgcttgcca gcagcacgtt
     6421 gtggctgtaa tcgagcagat tcaggtagcg gtcaaagatt ttggccgaaa atgcctgatc
     6481 gaggtcgaac tggcgataat gagaacgggt gaagcgcgac gttacgcgct cacttaccgt
     6541 cgcatgctgc gtctcttcct ttaataccgg aatttgatca gcacgcgtga tatcttctac
     6601 agcgaaggtc tggcctgcta ttgcaagcag gccagctaac gcggtaagcc taaaaaacat
     6661 gttcatgcct ggcccggcct ccgtttcaga acaccaggtg ttctgcgcgc acaatcaaag
     6721 acatacccga attcagctgg acgcggacgc cgtctttggt gatttctaat acggtggcat
     6781 ccatcgcgtt ttgacccgct ttcaccttca gggcttgtcc gacagtcaga gctgaaatgt
     6841 cagaaaccgg ggtgtgctgt tcttcgcgag gtgcttttac tgtttttggc gctttctcta
     6901 ccggcttttg cgcacgaggt ttacgttcag cgccttcttt gcggcgtggc gtggtcggac
     6961 gtggcttgcg ttcgcggcgc ggtgcgtctt ctttctcacc agcagctgcg gcagcttcgc
     7021 gttttttcgc ttgctgttca gcacgctgtg cctgaacacg cgctttcgct tcttcaagct
     7081 gcttgcgagc atgctctaca tgttgctcgt ccagctcacc gcatgggttg ccgtcaagat
     7141 cgacacgcgt tgcgccgggt ttaacaccgt aaagataacg ccagctcgaa gtgtagagac
     7201 gtaaagcgga tcgtaattgc gttttgctca ggttcatttc cccggcaaca cgatcgacca
     7261 aatcctgaaa aataccgatt ttcagcggac gcgcttcacc ttccgcactg aaacagtggg
     7321 gaaaacgttc ggccagaaac gcgattactt ctttactgct attcaactta ggttgatttt
     7381 ccatgaaatt tcctgattac aacggacgta gccaacaagc gcaggcatga acaggcgtca
     7441 ttataatgac gctaccagta aatgctacgt tatccgttga ttatcctgcg acgctcgcaa
     7501 agaatttttt gtaatccgtc gttgcaagca ctttttcaag ctgtgccaca agctgacgta
     7561 agccttgctc gtcctcgtct gtaaagcgac cgaagacggt actatcgatg tcaagaacac
     7621 caataatctg atttttcacc accagcggca gaacaatttc agaattactc gccgcatcac
     7681 aggcaatatg cccgtcaaac gcgtgaacat cctcgatacg ctgcacttga ttgcgggcaa
     7741 ccgcagtgcc gcacaccccg cgcccgacgg gtatccggac acaggcaatt ttgccctgaa
     7801 atggtcctag taccagtgta tcgtcctcaa gcaaataaaa acctgcccag tttacgtcag
     7861 tgagacgctc gtataacaac gcactggtgt tcgcaagcgt tgccagaaaa ctggtttctc
     7921 ccgccatcag cgcgttaaag tcgcgattta aatccgcgta aaattctgtt ttgttcatta
     7981 tataatcact tggttgtctt acctggaact gccagcctat taaaataagc attaaatgcg
     8041 ttaatgctca agatcattcc aatcatgggt taatattaat gttaattctt attacatttg
     8101 gcacgtcatg gctcttaaca caccacaaat tacgccgaca aaaaagataa cagtgagggc
     8161 aatcggcgag gaactgccgc gtggtgatta ccaacgttgc ccgcaatgtg acatgctgtt
     8221 tagcctgccc gagataaatt ctcatcagag tgcctattgt ccgcgctgtc aggcaaaaat
     8281 tcgcgacggg cgcgactggt cgctaacgcg cctggcggca atggctttca ccatgctgtt
     8341 gctgatgccg tttgcctggg gcgaaccgct gttgcatatc tggctgttag gtattcgtat
     8401 cgacgccaac gtcatgcaag gcatctggca aatgaccaaa cagggcgata cgataacggg
     8461 ggcgatggtc tttttctgcg ttattggtgc ccccctcatt ctggtgtcct ccatagcata
     8521 tttatggttt ggtaaccgac tgggaatgaa tttacgtccg gtactgctga tgcttgagcg
     8581 acttaaagag tgggtaatgc tggatatcta cctggtcggc attggcgttg cttctataaa
     8641 ggtacaggat tatgcccata tccaggcggg tgtcggcttg ttctcttttg tggcgttggt
     8701 gattttaacg acggtgacgt tgtcacatct taatgtcgag gagctgtggg agcgatttta
     8761 tccgcagcgc cccgctacgc gtagggacga gaaacttcgt gtctgtcttg ggtgccattt
     8821 taccggctat cctgatcagc gtggtcgctg cccgcgttgc catatcccgc tacgcctgcg
     8881 tcgccgtcat agtttgcaaa aatgctgggc ggcgctgtta gcgtcaatcg ttttgttgtt
     8941 acctgccaac ctgttgccta tttctatcat ttatctgaac ggaggacggc aggaagatac
     9001 aattctttcc ggaattatgt cgctggcaag tagcaacatt gcggttgcgg gaatcgtgtt
     9061 tatcgccagt attctggtac cgtttactaa agtgatcgtc atgttcactt tactgttgag
     9121 cattcatttt aaatgccagc aaggtttacg cacacgcatt ctgttactgc ggatggtgac
     9181 ctggattggt cgctggtcga tgctcgacct gtttgtcata tctttaacca tgtcgctgat
     9241 taatcgcgat cagatcctcg cttttactat gggaccggct gcgttttatt tcggcgcagc
     9301 ggtaattttg actattcttg ctgtggaatg gctggacagc cgcttacttt gggatgcaca
     9361 tgagtcagga aacgcccgct tcgacgactg aagcgcagat taaaaataaa cgccgtatct
     9421 cacctttctg gctgctgccg ttcatcgcgc taatgattgc cggttggctg atttgggaca
     9481 gttatcagga ccggggtaat accgtcacca tcgactttat gtcggcggat ggtattgtcc
     9541 cgggccgtac gcctgttcgt tatcagggcg ttgaagtcgg aacagtgcag gatatcagcc
     9601 tcagcgacga tcttcgtaag attgaagtca aggtcagcat caagtccgat atgaaagatg
     9661 cgctgcgcga agagactcag ttctggctgg tgacgccaaa agcatcgttg gcaggtgtct
     9721 ccgggctgga cgccctcgtc ggtggtaact atatcggcat gatgccaggt aaaggtaaag
     9781 agcaggatca ctttgtcgca ctcgataccc aaccgaaata tcgactggac aatggcgatc
     9841 tgatgatcca cctgcaagcc cccgatctcg gttcgctgaa cagcggttca ttggtctatt
     9901 tccgcaagat ccctgtggga aaagtctacg actatgccat caatcccaac aagcaaggcg
     9961 tggtgattga tgtcctgatc gagcggcgtt ttaccgatct ggtgaaaaaa ggtagccgtt
    10021 tctggaacgt ttccggcgtt gatgccaatg tcagtatcag tggcgcgaag gtgaaactgg
    10081 aaagtctggc ggcactggtt aacggtgcga ttgccttcga ttcacctgaa gagtcgaaac
    10141 ctgccgaggc ggaagatacc tttggtctgt atgaagatct ggcccacagc cagcgtggcg
    10201 taataataaa actggaactg ccgagtgggg caggattaac cgccgactcg acgccattaa
    10261 tgtatcaggg actggaagtc ggacagctaa ctaaactgga tttaaatcct ggtggtaaag
    10321 tcactgggga aatgacagtt gatcccagcg tcgttaccct gcttcgtgaa aatacccgca
    10381 tcgaattacg taatccgaaa ttatccctta gcgatgccaa tctcagcgcc ctgctgactg
    10441 gcaaaacctt cgagttggta cccggcgatg gcgagccacg caaagagttc gttgttgtgc
    10501 caggcgaaaa agcactgctg catgaacctg atgttctgac gctgaccctg accgcgccgg
    10561 aaagttacgg tattgatgct ggtcagccgc tcattcttca cggcgtgcag gtaggccagg
    10621 ttatcgatcg taaactcacc agcaaaggcg tcacctttac cgtcgccatc gagcctcagc
    10681 atcgagaact ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc gacgtgaagg
    10741 tggggctgga tggcgttgag tttctcggtg ccagcgcctc agaatggatt aacggcggga
    10801 tacgtattct gccgggcgat aaaggcgaga tgaaagccag ctatccactg tatgccaatc
    10861 tggaaaaagc gctggagaac agccttagcg atttacccac cacaaccgtg agtttgagtg
    10921 cagagacgct gccggatgtg caggcaggat cggtagtgct ctaccgtaaa tttgaagttg
    10981 gtgaagttat taccgtgcgt ccgcgagcta acgcgtttga tatcgatctg catattaagc
    11041 cggagtatcg caaccttctg accagcaata gcgtgttctg ggcagaaggc ggggcgaaag
    11101 ttcagctgaa tggtagtggc ctgaccgtac aggcatcccc gctctccaga gcattaaaag
    11161 gggctattag cttcgataac ctcagtggtg ccagcgccag tcagcgtaaa ggcgacaaac
    11221 gtattctgta tgcttccgaa acagcggccc gtgcggttgg tgggcagatt acgcttcacg
    11281 ctttcgatgc cggaaaactg gcggtcggga tgccaattcg ctatctcggt attgatatcg
    11341 ggcaaatcca gacgctggat ctgattaccg cgcgcaatga agtacaggca aaggcggtgc
    11401 tctatccgga atatgtccag acctttgctc gcggtggtac gcgcttctca gtggtcacac
    11461 cgcaaatttc ggcaactggc gttgagcatc ttgatactat cctccagccg tatatcaacg
    11521 tcgaaccagg ccggggcaat cctcgccgcg actttgaatt acaagaggcc accattactg
    11581 attcgcgtta cctggatggc ttaagcatta ttgttgaagc gccggaagcc ggttcgttag
    11641 gtattggtac gcctgtgctg ttccgtggtc tggaagtcgg tacggttaca ggaatgacgc
    11701 tggggacatt gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc
    11761 tggtgcgtaa caattccgtc ttctggttgg catcgggtta cagtctggac tttggtctga
    11821 cgggcggcgt agtgaaaacc ggcaccttta accagtttat ccgtggcggc atcgccttcg
    11881 ccacgcctcc aggtacgcca ctggcaccga aagcccagga aggcaaacac ttcctgttgc
    11941 aggaaagtga accgaaagag tggcgtgaat ggggaactgc gcttcccaaa taatgcccac
    12001 tgctccggcg tgcctgcgcc ggagcgttta tgctaaactg cgcgcctgtt tttttgccag
    12061 tggtacatgc tcgtggccca acacaccgtt tatttcccgg acgcctttct gacacaaatg
    12121 cgcgaagcga tgccttcgac gctctcattt gatgattttc ttgccgcctg tcagcgcccg
    12181 ttgcgccgca gcattcgcgt taatacgctg aaaatctccg ttgctgattt cctgcaatta
    12241 accgctcctt atggctggac gcttacgcca attccgtggt gtgaagaagg tttctggatt
    12301 gaacgcgaca atgaagatgc attgccattg ggtagtaccg ccgagcattt aagcggcctg
    12361 ttttatattc aggaagccag ttcaatgttg cccgttgccg ccttgtttgc tgacgataat
    12421 gcaccacagc gggtgatgga tgtcgcagct gcgccaggct ccaaaacgac gcaaattgcc
    12481 gcgcggatga ataacgaagg ggcaatcctt gccaatgagt tttccgccag tcgggtaaaa
    12541 gtgttacatg ccaatatcag ccgctgtggc atcagtaatg ttgcgctcac acattttgat
    12601 ggccgcgtgt ttggtgcggc agtgccagaa atgttcgatg ccattttgct ggacgctccc
    12661 tgctctggcg aaggcgtggt gcgtaaagat cccgatgcgc taaaaaactg gtcaccagaa
    12721 agcaatcagg aaatcgcagc tacacaacgg gagcttatcg acagcgcctt tcatgcatta
    12781 cgtcctggtg gtacgctggt ttactcgacc tgtaccttaa accaggaaga aaacgaagcc
    12841 gtttgcctgt ggctgaaaga gacttacccc gacgcagtag agtttttacc acttggcgat
    12901 ctcttccctg gtgcaaacaa agcgctgacc gaagaaggct ttttgcatgt tttcccacaa
    12961 atttacgact gcgaaggctt cttcgttgct cgtctgcgta aaactcaggc gatccccgcc
    13021 ttacccgccc ccaaatacaa agtcggtaat ttcccgttca gcccggtgaa agatcgcgaa
    13081 gccggacaaa ttcgtcaggc ggctgcaagt gttggcttaa actgggatgg aaacctgcga
    13141 ctctggcaac gcgacaaaga actgtggttg ttcccggtgg gcattgaagc cctgatcggt
    13201 aaagtccgat tttctcgctt ggggattaaa cttgccgaaa cgcacaacaa aggttatcgc
    13261 tggcagcatg aagcagttat tgcccttgcc acccccgaca atgtgaacgc ttttgaactg
    13321 acaccgcagg aagcggagga gtggtatcgc gggcgcgatg tttacccgca agccgcgcca
    13381 gtggcggatg acgtgttggt tactttccag catcaaccga ttggtttagc caaacggatt
    13441 ggttcgcgat tgaaaaacag ctatccgcgt gaactggtgc gcgatgggaa actttttacc
    13501 ggtaacgcct gacagcgcac aaaaaaagcg cactttttga ctggcacatt cggctgcctc
    13561 aactaggctg aaaaatggtg cgatcggact ggtcgtacca caaccggcag ctaaatggag
    13621 agcacgaaga tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta
    13681 cacggtgaat cgccgggtga tcgaacgtta accattcctt gtaaatctga ccccgattta
    13741 tgtatgcaac tcgatgcctg ggatgctgaa accagtatcc ctgccctgct aaatggcgaa
    13801 cactctgtcc tttaccgtac ccgttacgat caacaatctg atgcctggat tatgcgtctt
    13861 gcctgatcca aaaagaaccc gtcggcatgg cgggttattt gtcctggtta ttcccccgtt
    13921 gtaaaatctc tcctaaactt aacggtacgg caccacactt cggggatgaa atgttcgcgc
    13981 tggtactttt tgtttgctac ctggatggcg gttgtgaaga tattgttgtg gatgtctaca
    14041 acacggaaca gcagtgtctt tattctatga gcgatcaacg gatccgccat ggcggttgtt
    14101 ttccgattga ggattttata gatggtttct ggcgaccagc acaggagtat ggtgattttt
    14161 aattattgca attgcacaag agtcagttcg cccccaaaga cagcaccggt atcaatataa
    14221 tgcaggttgc caatatccac gcgatgtcgc aacggtgtat gaccaaacca gaaatgatca
    14281 gcacctgtaa ttccctgccc tttttggcgt tcacctaatc gcgagcggct ccacaagacc
    14341 tgatgcaaat caacgtcctt ttgccattca taaacatcat ctggataatc ggcatgagca
    14401 ataacatgtt tgccggtacg actgtgtact tcaagaataa agggcaaatg ctgacatttt
    14461 tccagcgccg ttttcgcttg tttctgttga ttatctgcca gcgcaataaa ccagtcgccg
    14521 ccattcatca accacaaaga catctgctgg gatgccagcg catccatcgc catctgttca
    14581 tgattgcctc ttaccgcacg aacccaatgt tgttccagta actgcaggca acgtaaactt
    14641 tgcggcccac gatcgataac gtctcccact gagataagta aatctcgcca cggatcaaaa
    14701 ccacaatgcc ataatttgcg gcgcaactgc tcaagacaac cgtgtatatc gccagaaagc
    14761 cagatatgtc gccattgatg acccgcaatt ctctgataaa cgggcgcagg ctgtttcatc
    14821 aatattttcc tcccgcgcta aagatcacat aatcttaaca agaatgttaa aaaacgctgg
    14881 actcagacag tagagtgtgt gttatggttg actataaagt cagcgaagga aatgcttctg
    14941 gcttttaaca gataaaaaga gaccgaacac gattcctgta ttcggtccag ggaaatggct
    15001 cttgggagag agccgtgcgc taaaagttgg cattaatgca ggcttagttg ccttgccctt
    15061 taagaataga tgacgacgcc aggttttcca gtttgcgtgc aaaatggtca ataaaaagcg
    15121 tggtggtcat cagctgaaat gttaaaaacc gcccgttctg gtgaaagaac tgaggcggtt
    15181 tttttattgg aaatcaaaag gctattttag gtaattaaca gagtttttca gctcgttcta
    15241 taaacggtgc cagactcatt ttttcgccgg gattgttagg atcatcaatc tgaatcaccg
    15301 aaatgggttg ggctttagtc ttcccactgg caacttcctt ttgtgcgata tcgtttaaag
    15361 gatactgcac gagggtactt gggttgatga catacaaagc attacccggt cggcaagtca
    15421 gcatcacctc ttcgcgatta aacgcccatt tgtctttacc cacttcaaaa cggctgacgg
    15481 taatcacctg cggtgcagcc agcgccgctg cagaactggt gagtaacaga aacgccagaa
    15541 tacttttttt catcattttc gtaatccatc aaaaaggttc gagagtcgca agcaggctga
    15601 tgatcaccag cactaccgca cctatcgccc attcgagttt agtcatccac tcaaaccagg
    15661 gtgccgcacg atcttcatcc tgcctcatgc gcggtacaag aacataacga ttagccagcg
    15721 caatgaccac cataatcatc accaggatgg ctttgagcaa taagagttgg ccccagtaag
    15781 tcgtaagcgt ggggggaaat ccggtaatca acaatgcatt aagcacgccg cttgccagta
    15841 cgccgatcac cgcaaaatgc ccgcaccagg aaaaacgcat cagcgcctga atggcctggt
    15901 gtcgccaacg gcctttgatg agttgcatac accagagcac cggcagtaat cccccaaacc
    15961 acgccgccgc acaaatcagg tgaatcgcgt gattagtctg atggattttc gccgttaccc
    16021 cttcattcag tgtcgcatgc ccgattcccg ccagcagaat aaattgcgcg gtagtgagca
    16081 taaacagcaa acgtggcata ttccgcggtt gcataagggc gacgatcaac gttacgaggg
    16141 cgagaacaat ttgccataac cagacgccac caaactgcgt ttgcaaaaca gcctgccaaa
    16201 tgtttggcga aaatacatct gcccatcccg ttcccatcaa cccgccctga acggcgagca
    16261 ttgcagtggc actgatcaaa ctccagacgg cagcgtgctg ttgtaaacgc agaaaacgct
    16321 tcgccagcaa gcgacgaatc gttaaggggg ccagccaggc accgtacatt gcgaagccaa
    16381 agaccagcat cagcgaggtg aaatggataa atcgcagcgc gatccaggta aacgccagca
    16441 tgatttattt cacgctaaag gtgtagtgtc ctttcgtttt atgcccgtcc accgaaacaa
    16501 catgccagtc aacggtgtag gttccgggtt tcagcgaatc ggccagtgga acaatcagtt
    16561 gcttttggtc ctgctcattt cgcttcgccg gtaatgtttt aatattttcg ttttttggcc
    16621 ccgtgatttt tgcaccactg aatcctgttt caacaccttc cgagaagttt aaagtgattg
    16681 cctgcggtgc agctgtcact tgcgcgtttg ccgcaggata ctgatgcgtt aaatgcgcat
    16741 gtgcccagac ggaaggtgtc accaacgaag tggtcaggat tgccagcgcg tagcgaaggg
    16801 agcgtgcagt tgaagtcata ttatctattc ctttttgtaa taactttttt acagagcata
    16861 accttgtcta atgtctgagt cgaggatcat caattccggc ttgccatcct ggctcactct
    16921 tagtaacttt tgcccgcgaa tgatgaggag attaagaatg ctgaagaatc tggctaaact
    16981 ggatcaaaca gaaatggata aagtgaatgt cgatttggcg gcggccgggg tggcatttaa
    17041 agaacgctac aatatgccgg tgatcgctga agcggttgaa cgtgaacagc ctgaacattt
    17101 gcgcagctgg tttcgcgagc ggcttattgc ccaccgtttg gcttcggtca atctgtcacg
    17161 tttaccttac gagcccaaac ttaaataaaa cttatacaga gttacacttt cttacataac
    17221 gcctgctaaa ttatgagtat tttctaaacc gcacccataa tttgcagtca ttttgaaaag
    17281 gaagtcatta tgtcgttctg gaaagttgca gcagcgcaat atgagcctcg taaaacctca
    17341 ctcaccgaac aggtagctca tcatctggag tttgtgaggg ccgccgccag gcaacaatgt
    17401 cagctgttag tttttccatc gctttcttta ctggggtgtg attattcgcg acgtgccctt
    17461 cctgccccac ccgatctctc actgttagac ccgctttgtt atgccgcaac gacctggcga
    17521 atgaccatca ttgccggcct tcccgttgaa tataacgatc gctttattcg tggaattgcg
    17581 gtgttcgcac cgtggcgaaa aacgcctggg atctaccatc agagtcatgg tgcatgtctg
    17641 ggccgtcgtt ccagaaccat cacggtggtc gatgaacaac cgcaaggtat ggatatggac
    17701 ccgacctgtt cactgtttac tacagggcaa tgtctgggag aacccgacct gctggcgtcg
    17761 gcccgtcgct tacaattttt ttcacatcag tactcgattg ccgtgctgat ggcaaatgcc
    17821 cgtggtaaca gtgcactatg ggatgaatat ggtcgtctca tcgttcgcgc cgatcgtggt
    17881 tcattattgt tagtcggtca gcgttcatca cagggttggc aaggcgatat cattccatta
    17941 cgctaggctt tttcggcctg gagcatgcca tgttgcgcat tatcgataca gaaacctgcg
    18001 gtttgcaggg agggatcgtt gagattgcct ctgttgatgt cattgacgga aaaatcgtca
    18061 accccatgag ccacctggtg cgccccgatc gtcctattag tccacaagcg atggcgattc
    18121 atcgcatcac cgaagccatg gtcgccgata aaccgtggat tgaagatgtg atcccacact
    18181 attacggtag tgaatggtat gtcgcgcata acgccagctt tgaccgccgc gtactgcctg
    18241 agatgcccgg tgagtggatt tgcactatga aactggcccg tcgtttgtgg cctgggatca
    18301 agtacagcaa tatggcgtta tataaaacac gcaagctcaa tgtacagacg ccgccgggcc
    18361 tgcatcatca ccgcgcgttg tatgactgtt atatcaccgc cgcgttgctt atcgatatta
    18421 tgaacacctc cggctggacg gcagaacaga tggccgatat caccggacgt ccgtcgttga
    18481 tgacgacctt cacctttggc aaataccgtg gcaaagcggt ttccgacgtt gccgaacgcg
    18541 atccgggcta tctgcgctgg ttatttaata acctggacag catgagcccg gagctgcgtt
    18601 taacactgaa acattatctg gaaaatactt agtccgcagg cgtagcgggt aatgttccct
    18661 gcgccagcgc gaccagaaaa gcatattcca tcgctacgcc ttcgtacgat ttaaagcgac
    18721 cagatttacc gccatggcct gagtccatgt cggtacagag caataaaaga tggtcatcgg
    18781 ttttcagctc gcgcaattta gcgacccatt ttgccggttc ccaatattgc acctgagaat
    18841 cgtgcaaacc ggtcgttacc agtaaatgcg gataagcctg tgcggtgacg ttgtcatatg
    18901 ggctgtagct tttcatgtac tcgtaatatt gcggatcctg cggattcccc cactcttcaa
    18961 actcaccagt ggtaagagga attgattcat caagcatcgt tgttacaaca tcaacaaacg
    19021 gtacctgggc gataacgccg tggaataatt ccgggcgttg attaattgca acgcccatca
    19081 acatgccccc cgcactcccg cccatcgcat aacaaagcga aggagagcca tagcccagtt
    19141 ttaacaatgc atcgcaggca tcaagataat cattaaacgt atttttcttc ttcagaaatt
    19201 ttccgtcttc gtaccattgt tgccccagct caccaccgcc gcgaacatgg acaatggcgt
    19261 agacaaagcc acgatctaac aaactcaagc ggctaaaact gaaatcggca tcaatacttg
    19321 cgccgtaaga accatagcca tacaccagca acgggttgtg tcctttgcga aaatgtttgc
    19381 gatggtagac caacgaaacc ggaacttcga cgccatcacg ggcgactatc cacaggtgtt
    19441 cactgcggta attcgccgca tcaaaaccgg gcacttctgt ttgttttaat acacgacgct
    19501 caccggtatc catatccagt tcaaacaaag tatctggtgt ggtcatggaa gaataaccat
    19561 aacgcaatcg cgcggtttca ggttctggat tgtaggcaat ccaggtcaca taggccggat
    19621 catcaaaggc aataccaatg acttcccggg tcttgcggtt aatttggcgc aaactggtta
    19681 acccgcgctg acgctcttca accaccagcc agtcggtaaa cagcgtaaac ccttccagca
    19741 taatgttatc gcgtggcgga attaactctt cccactgttg ctcatcacgc aggcgggtac
    19801 ggtataagcc aaagtttttg ccatggcggt tggaacgcag ataaaaccga tgctggtagt
    19861 gatcaaggct gtattcgtga tctttgcggc gcggcagaaa aacaaacggc tcggcatcgg
    19921 ccatttccgc gtccagcagg cgaacttcac tggtggtggc gctggccaaa tgaatgacta
    19981 catagtgctt cgaggtcgtt ttatgcaggc tgacgtaata ggtatcgtct ttttcttcgt
    20041 agatcagttt atcttgcgat gctggcgtac cgatggcgtg acgccagacc tgataaggca
    20101 gcagcgtcac cggatgcttg cgaacatagt agaaagtcca ggagtcattt gcccagacaa
    20161 agctgggttc aacgttatcc agcagttccg ggtaccagtt accagtttcc agattacgaa
    20221 aacgaatgcc gtactggcgt cgggaaagaa aatcttctgc cagcgccata atggtgttat
    20281 cgggcgtaat cgccattccg cccatcgaat aaaactcact atgagctgcg cgcttattgg
    20341 catcgagcaa tgtttcccac tcatcccact cttcactgaa tgccgattga cgctggtaga
    20401 tagcatattc acagcctggt tcataaatat gccgatagcg gtagccattt ttgatgtagg
    20461 gcgcagaaac ttctcgttgc ggaatgcggt cgatgatttc ctttaagatg cgatcctgca
    20521 aggcttgttg tgaggccatc acccgatgac cgtaactatt ttcttgttgc aggtagtcca
    20581 ggacttctgg ctgagaacgc gtatcgtccc gcagccagta gtaattatcg atgcgcgtat
    20641 cgccatgaag cgtcatggcg tggggaatgc gggcggcttt tggtagcatg ttattgttct
    20701 ttctggttga aacatcttat aagggtggca aaactcaccg gggatgcaag cgaaacaggg
    20761 gagtcattgc ttagatgatg acaggtaatg gcgcggatat cgaatgttat gcagacagag
    20821 aaaatcagcc tgttcaaatg gctgtgcgat tctggatagc ccgaaatagt caacttcagg
    20881 ctatccagag agcggaatta ttccgccaaa gtgcgttttt gctgttcgag atcgcgttca
    20941 atgccgtcac gaacatcctg ggggattttc agcgcgtcac ccagtgcatt caggtaactg
    21001 cgttccataa aatggtcaat atcaatagcc gcgcaactca ggaaatagat ttccagcgcc
    21061 tcttcttcat tgcggacccc ggtagccagg cgttgtggat ccagcggttg ttcgattgcc
    21121 tgctcaatga gtacacgccc ctgctcttcc acgccggctt cacgcaattg ctggtcgatt
    21181 gccgcacgtt ctttggcatc aatatgacca tcacttttag cggcaaagac cagcgcaagg
    21241 atcaaacgtt ctgtacgctc atccagcggc gtactttgcg cgccaaactg cggttcgtcc
    21301 tgatgcgccg cgcgaatttt atctttgtat ttattccaca gcaccgtacc cgctaccgct
    21361 ccgccgccaa ccagtaacgc gttggtgcca tatttcgtaa gaagtttacg tgctgattta
    21421 tttgcgacca gcagcccagc cagtccgcct aatgcgcctg gcactaacag tttgcccaat
    21481 ccctgatccg cagacgagga ggtagaagaa ctgctttgcc caagcaggga ttgcagttga
    21541 tttaaccagt tagccatatt tgctcctcaa taaccatttt tagtgcctgt agcatagcgg
    21601 atgaggatgt cagaaaatgt atatcgagac aaaagatgcg caaataagcg gggcggtagg
    21661 ccgttattca aagaaattcg cgccaggtaa ggtatttact gtatatcagg tgataagggt
    21721 attttaggtg aaagtagttt gtcgttttga gtggcattgt ttgatgctga ttgcccgatg
    21781 cgacgctgtt gcgtcttctc aggcctacga ttgtggtgcg ttctggggcg gacaaggcac
    21841 tcgcgccgca tccgccaacg gtcaatcgaa ttatcagaag ccattaacgc cgctgatatt
    21901 ccgccattcc cgctttgcag tccacgctga cctggtaatg aatatcggca cttttaccac
    21961 gcacggttag cggtactgac catttatcat ctttaccctg aatgtcctgc aaactgaccc
    22021 aagccacggg atcggcctga ccgacaattt tttgatcatc tgcccaacgc gccacgcgat
    22081 tttgttgata atcacgtttt acgctcgcgg caattccggc agcatccaga ccttcacact
    22141 ttgggaaagt gaccgacttg ctggtttcat tattggcagc gaaaactgat gcgcaggcag
    22201 aaaccaacaa cagccctaaa aacgcccctc tttttttcat gtttttctcc atagcacaat
    22261 gattcaggag aaagcatggt acaaattgtc aggagcgcaa gttgcttcag gccgcgtgtg
    22321 aggcatcttc ctcttcggat tcagcaccga cgacctgtgg ggacggtaat ttgccggttt
    22381 tcagaatggt gctcagcaca tctttttgtt ctgccagcca tagcgaaagg gcttcacgtt
    22441 gctcgtcttc catttgcact ggagaatttg tcagccaggt gtcaagcaga tccgccgtat
    22501 caagcatttt atcataagca tcggcttcct ttttgctggt aaacgacatt ttctcttcgc
    22561 cctcacgaat gactacgtat ttaacttcaa ccgccatttg cagcctctca taataactgt
    22621 gattttatac agtatatttc ttttcggttg agaaatcaac atcagcaata aagacacacg
    22681 caaacgtttt cgtttatact gcgcgcggaa ttaatcaggg gatattcgtt atgacgttat
    22741 taggcactgc gctgcgtccg gcagcaactc gcgtgatgtt attaggctcc ggtgaactgg
    22801 gtaaagaagt ggcaatcgag tgtcagcgtc tcggcgtaga ggtgattgcc gtcgatcgct
    22861 attccgacgc accagccatg catgtcgcgc atcgctccca tgtcattaat atgcttgatg
    22921 gtgatgcatt acgccgtgtg gttgaactgg aaaaaccaca ttatatcgtg ccggagatcg
    22981 aagctattgc caccgatatg ctgatccaac ttgaagagga aggactgaat gttgtcccct
    23041 gcgctcgcgc aacgaaatta acgatgaatc gcgagggtat ccgtcgcctg gcggcagaag
    23101 agctgcagct gcccacttcc acttatcgtt ttgccgatag cgaaagcctt ttccgcgagg
    23161 cggttgccgc cattggctat ccctgcattg taaaaccggt gatgagctct tccggcaagg
    23221 ggcagacgtt tattcgctct gcagagcaac ttgctcaggc atgggaatac gctcagcaag
    23281 gcggtcgcgc cggagcgggc cgcgtaattg ttgaaggcgt cgttaagttt gacttcgaaa
    23341 ttaccctgct aaccgtcagc gcggtggatg gcgtccattt ctgtgcacca gtaggtcatc
    23401 gccaggaaga tggcgactac cgtgaatcct ggcaaccaca gcaaatgagc ccgcttgccc
    23461 ttgaacgtgc gcaggagatt gcccgtaaag tggtgctggc actgggcggt tatggtctgt
    23521 ttggtgtcga gctgtttgtc tgtggtgatg aggtgatttt cagtgaggtc tcccctcgtc
    23581 cacatgatac cgggatggtg acgttaattt ctcaagatct ctcagagttt gccctgcatg
    23641 tacgtgcctt cctcggactt ccggttggcg ggatccgtca gtatggtcct gcagcttctg
    23701 ccgttattct gccacaactg accagtcaga atgtcacgtt tgataatgtg cagaatgccg
    23761 taggcgcaga tttgcagatt cgtttatttg gtaagccgga aattgatggc agccgtcgtc
    23821 tgggggtggc actggctact gcagagagtg ttgttgacgc cattgaacgc gcgaagcacg
    23881 ccgccggaca ggtaaaagta cagggttaaa cccggcaaaa aaacgctaca aaaatgcccg
    23941 atcctcgatc gggcattttg acttttacag cttagcgcct tctacagctt cacgcgccag
    24001 cttagtaatg cgatcgtaat cgcccgcttc cagcgcatct gccggaacca gccaggaacc
    24061 accgatgcac agcacgcttt tcagcgccag gtagtcacgg tagttagccg gagaaatacc
    24121 acccgtcggg cagaaacgga cctgggagaa cggacccgcg atcgcctgca gggctttcac
    24181 gccgccgtta gcttcagccg ggaagaattt gaactctttc aaaccgtagt ccatacccag
    24241 catcagttcg gaaacagtgc tgatccccgg aattagagga atagtccctt cggtagcagc
    24301 tttcagcagc ggctcggtca gacccgggct aattgcgaac tgtgcacccg cttcagtgac
    24361 ttctgccagc tgctgtggat tcagcaccgt accggcaccc acaatcgctt caggcacttc
    24421 tttggcgata gcacggatag cgtcaactgc acactcggta cgcagagtca cttccagaac
    24481 gcgcacccca ccagcaacca acgcttttgc catcggcact gcgtgttcca gtttttttac
    24541 cacgataacc ggtacaaccg ggccggtggt caggattgat tctgcacttg ttttccagtt
    24601 tttcatcaga gttttctctc gcctgattac aaatttgtcg tcttaaaaag tgatacaggt
    24661 tgcgccctgt tcggcaccgg acagtttttc acgcaaggcg ctgaataatt cacgtcctgt
    24721 ccccacgcgt gacgcgctca ggtcaggaat gtgcggttcg cgagcagcca gttccgcttc
    24781 gtctaccagc agcgtcagtt cgcctgtctg tccattcaca cgaatgatgt ccccgtcgcg
    24841 cacttttgcc agcagcccgc catcgtaggc ttctggtgtt acgtggatag ctgacggcac
    24901 tttacctgaa gcgccggaga gtcgtccatc ggtaactaac gcaattttga aacaccggtc
    24961 caataataca ccaagtggcg gcatgagttt atgtaattct ggcattccgt tcgcttttgg
    25021 cccctgatga cggacaacaa cgacacaatc gcggtccagc aaacccgctt caaaggccgg
    25081 cataacgtca tgctggcttt caaaaacaac cgctggcgct tcaatcacct ggttctcaac
    25141 cggcacggca gaggttttca taaccgcacg gcccaggtta ccgcttaaca cttttgtccc
    25201 accatgatga gagaaaggtt gttcgaagga agcgatcaca ttgctgtcga gtgatttttc
    25261 cgccccttcc cgccagtcca gttcaccatt attcagccat ggttcaaggg tataacgaga
    25321 cagaccaaag cctgccaccg tattgacatc ttcatgcagc aggcctgctt tgagcagttc
    25381 acgcaccaga accggtacgc cacctgccgc ctggaagtgg ttaatatcgg ccggaccgtt
    25441 cgggtagaga cgtgccatca gcggtacaac atcagaaagg tcagagaagt catcccagtt
    25501 aatctgaata ccggccgcgc gcgccatcgc caccaggtgc atggtgtggt tagtggaacc
    25561 accggtcgcc agcagtgcaa cgataccgtt caccaccact ttctcatcga tcatcttacc
    25621 gatcggcatc cattcattac cattaccggt catgcgtgta acctgacgcg cagcggcagc
    25681 ggtcaaagca tcgcgcagcg gagaatccgg atgaacaaaa gaagagcctg gcaactgcat
    25741 ccccataaat tccaccacca tctggttggt gttggcagta ccgtagaaag tacatgttcc
    25801 cggcgcatgg taagacgcgg cttctgactc cagtaaggcc atgcggtcca ctttaccttc
    25861 ggcataaagc tggcgaatac gcactttttc tttatttggc aaaccgcttg ccatcggtcc
    25921 agacggcaca aacaccgcag gcaaatgacc aaacgacagg gctgccatcg tcagacccgg
    25981 gacaatcttg tcgcacacac cgaggaacag agcaccatca aacatgttat gggacagccc
    26041 caccgccgca gacatcgcta tcacttcgcg gcttagcagc gacaattcca ttccatcctg
    26101 cccctgggtg acaccatcac acatcgccgg aacaccgccc gcaacctgac caaccgcatt
    26161 cgcttcatgc agggctttac gaatgatttc tggatagtgt tcataaggct ggtgcgcgga
    26221 gagcatgtcg ttataggagg tgatgatggc gatattgtta cgcaacatgc ttttcaaaga
    26281 ggctttgtct tctggctggc aggcagcgaa accgtgtgcc aggttaccgc atgccaactg
    26341 cgaacgatga acggtcgacg ttttcgcttg ttctatccgg gcgagataag cagagcgagt
    26401 ctcgcgcgaa cgttcaatga ttcgatttgt tacgcgtaac aattgtggat tcataaaggc
    26461 tcctgaaatt gagttgtcag agcaggatga ttcacaacgc gtttcattca gaggatttat
    26521 gagtgaaacg cctgtaaccg gagctcatag ggcaaaaacg tttcagtcag tgtaataaaa
    26581 aaagcctcgt gggtgaatcc gcacgaggcc tgaaagtgta aaaattgttc tacaatctgt
    26641 gcaagatcat gttaccggta aaataaccat aaaggataag cgcagatatt actcaaactc
    26701 attccaggaa cgaccatcac gggtaatcat cgccaccgag gcaacgggtc cccaggttcc
    26761 ggcctgatac ggtttcggcg catcattgtc catcgcccac gcctcagtaa tggagtctac
    26821 ccatttccag gcttcttcca cttcgtcgcg acgtacaaac agtgcctgaa taccacgcat
    26881 ggtttccagc agcaaacgtt cataggcatc cgccagatgc gtctgattaa aggtttctga
    26941 atagctcaga tccagcttgg tgatttgcag gttatgtttg tggtcaaggc caggaacttt
    27001 attcagtacc tggatatcca cgccttcatc aggttgcaga cggatagtca gtttattctg
    27061 cggcagatcc tgccacgact ctttaaacag attcagttca ggtgttttga aatagaccac
    27121 gacttcagaa catttggtcg gcagacgttt accagtacgc aggtagaatg gcacaccggc
    27181 ccagcgccag ttatcaatgt cgacgcggat cgccacgaag gtttctgtat tgctgctctt
    27241 gttcgcgccc tcttcttcca gatatcccgg cacttttttg ccctgggcga agcccgcagt
    27301 atattgcccg cgtacggttt tttcgcgtac gttgtagcgg tcgatgcggc gcagagactt
    27361 cagtactttc actttttcat cgcggatgct gtctgcgctc aggtcagacg gcggagacat
    27421 cgcaatcatg caaagaattt gcagcaggtg gttctggatc atgtcgcgca tctgaccggc
    27481 tttatcaaaa tagccccagc gcccttcgat ccccacttct tctgccacgg taatctcaac
    27541 atgatcaatg gtgcgattgt cccagttatt cacaaacagg gagttagcaa aacgcagcgc
    27601 caacaggttc agcaccgttt ctttaccaag atagtggtcg atacggtaaa cctggcactc
    27661 ctcgaagtat tcgccaacct gatcattgat ttcctgcgag gtcgccagcg acgtccccag
    27721 cggtttctcc atgactacgc gtgccggttt agcattcagt tttgcctcgc caagcccttt
    27781 gcaaattgcg ccaaaagtgc tgggcggcat ggcaaagtag ttaatggtga tacgattttt
    27841 ttgatccagc atcgcgccga gacggctgaa tgcagcagtg tcattgacat cgagattaca
    27901 aaaatccaga cgtgcactca gggtgtccca taaaccttca tcaatggttt ctttcatgaa
    27961 agtttcgagc gcctcgcgga caactttggt atatgccgct ttatcccagt cagcacgccc
    28021 tacgccgata atccgggtgt ccgggttgag ctgaccggct ttttccagtt gatacaggga
    28081 aggcagcaat ttacgacgcg caaggtcgcc tttcgcgccg aaaatgacca ggtcacaggc
    28141 ctgggctgtt tgcgttaccg ccatgtcatt ctccttaagt taactaaccc ggtacttaag
    28201 ccagggtata cttgtaattt tcttacggtg cactgtactg cttttacgag cttgcgaaaa
    28261 ctgtaaacgc ttatccaccc gtgcgattac gggaaaagcg cgcaaagtgc ggcaaaactg
    28321 ataaaaaaat catcgttttc cattgggtaa aaatctgaca ctgatcatgt tatgaaaaaa
    28381 aataacaact tttttatctg cttttgtcat taacacggca cacaggcgta atatctgaca
    28441 aaactgcatt tcgatttctt tcagtgcgga aatcgtcatt acccgtgagt ctctttacat
    28501 catgaatatg ctggaaaaaa tccagtctca gctggaacat ttgagcaaat cagagcgcaa
    28561 agttgccgag gtcattctgg cttcgcccga taacgcgatc cattcgagta ttgctgctat
    28621 ggcactggaa gccaatgtta gcgaaccgac ggtgaatcgt ttctgtcgca gcatggacac
    28681 gcgcggtttt cctgatttta aacttcatct ggcacagagt ctggcgaatg gcactcccta
    28741 tgttaatcgc aatgtcaatg aagatgacag cgttgaatca tacacaggga aaatatttga
    28801 gtccgcaatg gcaacccttg atcatgtccg tcattcactg gataaatctg ccatcaaccg
    28861 cgccgtcgac ttgctcactc aggcaaaaaa aatcgccttt ttcggattag gctcttcagc
    28921 cgccgttgcc cacgatgcga tgaataagtt ctttcgtttt aatgttccgg tggtgtactc
    28981 cgatgatatc gtgctgcaac gcatgagttg tatgaattgt agcgacggag acgtggtggt
    29041 gctgatttct cacactggaa gaacaaaaaa tctggtcgag ctggcgcagc tggctcgcga
    29101 aaacgacgcc atggtgattg ccctcacctc tgcgggtacc ccgctcgccc gggaagcaac
    29161 gctggcaatt accctcgacg taccggaaga tactgacatt tatatgccca tggtttctcg
    29221 acttgcacag ctgaccgtga tagatgtgct ggcgacagga tttactttgc gacgcggtgc
    29281 aaaattcaga gataacttga agcgggtcaa ggaagcgctg aaggaatcgc gttttgataa
    29341 gcagttactt aatttaagtg acgatcgcta aaaacgactg tcactgtcct aatcttatac
    29401 gacatccgaa tgagattaat ttatcgccat cgcggcgtta tttcattcgg atttcatgtt
    29461 caagcaacac ctggttgttt cagtcaacgg agtattacat gtccagaagg cttcgcagaa
    29521 caaaaatcgt taccacgtta ggcccagcaa cagatcgcga taataacctt gaaaaagtta
    29581 tcgcggcagg tgccaacgtt gtacgtatga acttttctca cggctcgcct gaagatcaca
    29641 aaatgcgcgc ggataaagtt cgtgagattg ccgcaaaact ggggcgtcat gtggctattc
    29701 tgggtgacct ccaggggccc aaaatccgtg tatccacctt taaagaaggc aaagttttcc
    29761 tcaatattgg ggataaattc ctgctcgacg ccaacctggg taaaggtgaa ggcgacaaag
    29821 aaaaagtcgg tatcgactac aaaggcctgc ctgctgacgt cgtgcctggt gacatcctgc
    29881 tgctggacga tggtcgcgtc cagttaaaag tactggaagt tcagggcatg aaagtgttca
    29941 ccgaagtcac cgtcggtggt cccctctcca acaataaagg tatcaacaaa cttggcggcg
    30001 gtttgtcggc tgaagcgctg accgaaaaag acaaagcaga cattaagact gcggcgttga
    30061 ttggcgtaga ttacctggct gtctccttcc cacgctgtgg cgaagatctg aactatgccc
    30121 gtcggctggc acgcgatgca ggatgtgatg cgaaaattgt tgccaaggtt gaacgtgcgg
    30181 aagccgtttg cagccaggat gcaatggatg acatcatcct cgcctctgac gtggtaatgg
    30241 ttgcacgtgg cgacctcggt gtggaaattg gcgacccgga actggtcggc attcagaaag
    30301 cgttgatccg tcgtgcgcgt cagctaaacc gagcggtaat cacggcgacc cagatgatgg
    30361 agtcaatgat tactaacccg atgccgacgc gtgcagaagt catggacgta gcaaacgccg
    30421 ttctggatgg tactgacgct gtgatgctgt ctgcagaaac tgccgcaggg cagtatccgt
    30481 cagaaactgt tgcagccatg gcgcgcgttt gcctgggtgc ggaaaaaatc ccgagcatca
    30541 acgtttctaa acaccgtctg gacgttcagt tcgacaatgt ggaagaagct attgccatgt
    30601 cagcaatgta cgcagctaac cacctgaaag gcgttacggc gattattacc atgaccgaat
    30661 cgggtcgtac cgcgctgatg acctcccgta tcagctctgg tctaccaatt ttcgccatgt
    30721 cgcgccatga acgtacgctg aacctgactg ctctctatcg tggcgttacg ccggtgcact
    30781 ttgatagcgc taatgacggc gtagcagctg ccagcgaagc ggttaatctg ctgcgcgata
    30841 aaggttactt gatgtctggt gacctggtga ttgtcaccca gggcgacgtg atgagtaccg
    30901 tgggttctac taataccacg cgtattttaa cggtagagta agtacgttgc cggatacggc
    30961 gaaaacgcca catccggcct acagttcaat gatagttcaa cagatttcga atattctgaa
    31021 gcaaacttga acttatcatc aggcgaaggc ctctcctcgc gagaggcttt tttatttgat
    31081 gggataaaga tctttgcgct tatacggctg gatttcgccc ggtttgcgag ttttcagcaa
    31141 ttttagtatc caggtgtatt gttctggtcg cggaccaaca aaaatctcga cttcttcatt
    31201 catccgccgc gcaatcgtat gatcatccgc ctctaacaga tcatccatcg gtgggcgcac
    31261 ctgaatcgtc agacggtgcg tcttgccatc ataaatcgga aacagcggaa caacgcgcgc
    31321 acggcacact ttcatcaaac gaccaatcgc gggcaacgtc gctttatagg tggcaaagaa
    31381 atcaacaaat tcgctgtgct cagggccatg atcctgatcg ggtaaataat atccccagta
    31441 gccctgacgt accgactgga tgaatggttt aataccatca tttctcgcat gcagacgacc
    31501 gccaaaacga cgacgcaccg tgttccagac ataatcaaaa accgggttgc cctgattatg
    31561 gaacatcgct gccattttct gcccttgcga ggccatcagc atggcaggaa tatcgacggc
    31621 ccaaccgtgc ggcaccagaa agataacttt ctcgttatta cgccgcatct cttcgatgat
    31681 ctccagccct tgccagtcaa cgcgcggctg aattttctcc ggcccgcgta ttgccaactc
    31741 agccatcatt gccatcgctt gcggcgcggt ggcaaacatc tcatcaacaa tcgcttcgcg
    31801 ttcagcttca ctacgttctg gaaagcagag cgacagattg attaacgcac gacggcgtga
    31861 gctttttccc agtcgtccgg caaaacgtcc cagccgtgcc agaatgggat cacggaactt
    31921 tggcggcgtt aaagcgatac ccgccatcgc tgctacgccc agccatgctc cccagtagcg
    31981 cgggtggcga aaggatttat caaactcagg aatgtattcg ctattatttt ttttcgtttc
    32041 catgcttttc cagtttcgga taaggcaaaa atcaatctgg tgatagtgta gcggcgcaac
    32101 ttgccccgca ccaaataaaa aaagccggta ctgacttcgt accggctgcg aatggatgtt
    32161 aattaatcaa accgtagctg cggcacaatc tctttggcct gcgccaggaa ttcgcgacga
    32221 tcggagccgg tcagcccttc ggtacgcggc agttttgccg tcagcgggtt tacggcctgc
    32281 tggtttatcc atacttcata gtgcaaatgc ggcccggttg aacgtccggt attaccggaa
    32341 agcgcgatac ggtcgccacg tttcaccttc tgtcccggtt tcaccagaat cttgcgcaag
    32401 tgcatataac gcgtggtgta gctgcgacca tgacgaatag ccacataata acctgctgcg
    32461 ccactgcgtt tggcaaccac cacttcaccg tcacccactg aaagcactgg cgtaccctgc
    32521 ggcatggcga aatcaacacc tctgtgtggt gcaacgcgac cggtcaccgg attagtacga
    32581 cgcgggttaa agttagacga gatacggaac tgtttcgccg tcgggaatcg caagaatcct
    32641 ttcgccagac cagtaccgtt acgatcgtag aatttgccat cttcagcgcg gatagcgtaa
    32701 taatctttac cttctgaacg caaacgtacg cccagcagct ggctttgctc acgtttacca
    32761 tcaagcattt ctcgagacat taataccgca aattcatcgc cttttttcag tttgcggaaa
    32821 tccatttgcc actgcatggc tttaatcact gcgctcactt cggcgctggt taaaccggcg
    32881 tttctggcgc tggcaacaaa gctccccccg acggtacctt tcagcagatt gttaacccac
    32941 tctccttgct gcatttcgct ggtcatttta aaaccgttag cggcagtacg gtcataggtt
    33001 cgggtttcac gacgagacac ttcccaggtg aggcgctgca gttcgccgtc cgcggttaat
    33061 gtccaggaga gttgttgacc gattttcagg ttacgcaatt ctttgtcggc agcagccagt
    33121 tgggtgatat cacccatatc aataccatac tgattgagaa tgctgcttag cgtatcgcca
    33181 gtggaaacaa catattcatg cacgcccgct tcaccggcga ttttgtcatc cagttcgtcc
    33241 tgaggaatgg cttcatcttc ttgtgcagct tgatcaatcg gctcactggc ttcaggtaag
    33301 agcgaacgaa tttcgttctg ttccagctca atggttttga caattggcgt ggcatcgcgg
    33361 tgataaacat agggccgcca gacagcgacg gccagagtaa gaacggtgag cgaccccaac
    33421 ataacgcggt gtggtcgcgg taaattatta aacgccaggg cgacagagcg ggctatctgt
    33481 tgcacgtaat cacttcctca ttaatctcct ttcaggcagc tcgcatactg gttggctaat
    33541 tgactcagga attctgaata gcttgtttta cccagtttga tattcgtccc caggggatcc
    33601 aacgttccca tacgaacgga tgtccctcgt gcgacgctct caacgaccgc tggcctgaac
    33661 tgtggctcag caaaaacgca ggttgctttt tgctcaacca actgtgttct tatttcatgt
    33721 aaacgctgcg cgccaggttg aatctcaggg ttaacggtaa aatgaccaag cggtgtcagt
    33781 ccaaactgtt tttcgaaata gccgtaagca tcgtgaaaaa cgaaataacc tttccccttg
    33841 agcggcgcga gctcgttacc aacctgcgtt tcggttgagg ctaattgtgc ctcaaaatcc
    33901 ttcaggttgg cgtcaagttt ggctcgactt tgcggcataa gttccactaa ttttccatgg
    33961 attgcaaccg ctgtagcccg cgctatctct ggggaaagcc aaagatgcat gttgaaatcg
    34021 ccgtgatggt gatcttcgtc acttttttcc gcgtggtcgt gatcatcatc atcgccgtga
    34081 atacttttca tcagtagcgg tttcacatct tcaagctgcg caatcgttac ctgcttcgct
    34141 tctggtaatt tgcttaccgg tttttgcata aacgcttcca tctccgggcc aacccaaacg
    34201 actaagtccg cgttctgtaa gcgttttaca tccgatggac gcagtgaata atcatgttct
    34261 gaagcgccgt caggcagtaa aacctccgtt tctgttacac catcagcaat ggcagaagcg
    34321 atgaacccaa cgggtttaag cgaagcgaca acggcggcat ctgcggcctg tgttgcacct
    34381 ccccagagag cggcggataa tgctgcgaaa agaagcgttt ttttatgtaa cataatgcga
    34441 ccaatcatcg taatgaatat gagaagtgtg atattataac atttcatgac tactgcaaga
    34501 ctaaaattaa catgacaagt ctggtttccc tggaaaatgt ctcggtttct tttggccaac
    34561 gccgcgtcct ctctgatgtg tcgctggaac ttaaacctgg aaaaattttg actttacttg
    34621 ggccaaatgg cgcaggtaag tcgacactgg tacgggtagt gctcgggctg gtaacacccg
    34681 atgaaggggt tatcaagcgc aacggaaaac tgcgcatcgg ctatgtaccg cagaagctgt
    34741 atctcgacac cacgttgcca ctgaccgtaa accgtttttt acgcttacgc cctggtacac
    34801 ataaagaaga tattttgcct gcactgaaac gtgtccaggc cgggcacctg attaacgcac
    34861 cgatgcaaaa gctctctggt ggcgaaacgc agcgtgtact attagcgcga gcattgttaa
    34921 atcgcccgca attattagtg ctggatgaac ccactcaagg cgtggatgta aatggccagg
    34981 tggcgttata tgaccttatt gaccaactgc gtcgtgaact ggattgtggc gttttaatgg
    35041 tttctcacga tctgcatctg gtgatggcaa aaaccgatga agtgctgtgc ctgaatcacc
    35101 acatttgttg ttccggcaca ccggaagttg tttccctgca tccggagttt atttcaatgt
    35161 ttggtcctcg tggtgctgaa caactgggta tctatcgcca tcatcataac catcgtcacg
    35221 atttacaggg acgaattgtt ttgcgtcggg gaaatgatcg ctcatgattg aattattatt
    35281 tcccggttgg ttagccggga tcatgctcgc ctgtgccgcg ggtccgctgg gttcgtttgt
    35341 agtctggcgt cgtatgtctt atttcggtga tacgctggct catgcctcat tacttggcgt
    35401 cgcgtttggt ttgttgctgg acgtgaatcc attctatgcg gtaattgccg ttacgctgct
    35461 cctggcgggc ggtctggtat ggctggagaa gcgtccacag ctggcgattg acacgttatt
    35521 agggattatg gcgcacagtg ccctgtcgct gggcctggtg gtcgttagtc tgatgtctaa
    35581 cattcgtgtt gatttgatgg cttacctgtt cggtgatttg ctggcagtga cgccagaaga
    35641 tctcatcgct attgcgattg gcgtggtcat cgtggtggct attttgttct ggcaatggcg
    35701 caatttgctg tcgatgacga ttagcccgga tctggcgttt gttgatggtg tgaaattaca
    35761 gcgcgtgaaa ttgttgttga tgctggtgac ggcattgacg attggtgtag cgatgaaatt
    35821 tgttggcgcg ttgattatta cttcactgct gattattcct gcagctactg cgcgtcgctt
    35881 tgcccgtacg ccggaacaga tggctggtgt cgctgttttg gtggggatgg tggcagtaac
    35941 tggcggttta accttttccg cgttttacga tacgccggcg ggtccgtcgg tggtgctatg
    36001 tgcggcactg ttatttattc tcagtatgat gaaaaagcag gccagctaat ctatcgctga
    36061 acacatttgt cggatgcggc gcgagcgcct tatccgacct acggttcgct atctctggta
    36121 ggccggataa gacgcgaata gcgtcgcatc aggcacactg ccaatgtcgg atgcggtacg
    36181 agcgaccaat ccgacttacg gcatttctgg cggcgttatg ccaaagtgat tccacgcccg
    36241 cgtcgtcgcc atacgcccac gcggtgtacg ctgcaaaaag ccttgctgaa tcagataagg
    36301 ctccagcaca tcctcaatgg tttcacgttc ttcgccaata gccgccgcca ggttatccag
    36361 acctacaggc ccaccaaaga atttatcgat taccgccagc aacaatttgc ggtccatata
    36421 atcaaaacct tcagcatcga cattcagcat atccagcgcc tgagcagcga tatctgccga
    36481 gatggtgcca tcgtgcttca cttcggcgaa atcacgcact cgacgcagca gacggttggc
    36541 aatgcgcggc gtaccgcgag cgcgacgagc aacttccagc gcgccgtcat cactcatctc
    36601 aagccccata aagcgtgcgc tgcgactgac gatatattgc agatccggca cctgataaaa
    36661 ctccagacgt tgcacaatac caaaacggtc gcgcaacggt gatgtcagcg aacctgcgcg
    36721 tgtggttgca ccaatcaggg taaacggcgg caaatcaatt ttaatggagc gtgccgccgg
    36781 accttcacca atcatgatat ccagttggta gtcttccatt gccggataca acacctcttc
    36841 aacaactggc gatagacggt ggatctcatc aataaacagc acgtcatgcg gttcaaggtt
    36901 agtgagcatc gctgccagat cgcccgcctt ttccagcacc ggaccagaag tcgtgcgtaa
    36961 attaacgccc atttcattgg ccacaatgtt ggcaagcgta gttttaccca accccggagg
    37021 accaaaaatc aacaaatgat cgagggcatc gccgcgcagt ttcgctgctt tgatgaaaat
    37081 ctccatctgt gaacgaacct gcggctgacc aacatactct tccagtaatt tagggcgaat
    37141 ggcgcgatct gctacatctt ccggcaaagt ggtaccggca gaaatcagac ggtctgcttc
    37201 aatcatcctt tacctcataa cgcggcgcgt agggcttcgc gaattaatgt ttcactgctg
    37261 gcgtcagggc gagcgatttt gctcaccatg cggcttgctt cttgtggttt atagcccagc
    37321 gccaccagcg cggcaaccgc ttcttgttca gcatcgtcgg tcgccgggct ggcaggagac
    37381 gtgagcacca gatcggcggc tggcgtaaag agatcgccgt gcaaaccttt aaatcggtct
    37441 ttcatttcga caattaaacg ctcggcggtt tttttaccaa tgcccggcag tttcaccagt
    37501 gctcccactt cttcacgttc aacggcatta acgaactgct gcgctgacat tccggagagg
    37561 atcgccagcg ccaacttcgg gccgacgccg ttggttttga ttaactcttt aaacaacgtg
    37621 cgctcttgct tattgttaaa gccgtacagc agctgcgcgt cttcacgcac cacaaagtgg
    37681 gtgaaaacga tcgcttcctg acccgcttca gggagttcat aaaaacaggt catcggcata
    37741 tgcacttcat agcctacgcc gcccacttca attaacacca gcgggggttg tttttcaatg
    37801 atgatgcctc tgagtctgcc tatcacatga cgctcctgcg taatgaatca aagataatgc
    37861 tgtatgataa aaaaatgctg gatagatatc cagcgaagga tgaagaaaac ttgcgaggcg
    37921 tcccaacgat ctgaaaaatg gcgcagtata atttattcta caggtta
//
LOCUS       DAZFXT010000035        37619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.035, whole genome shotgun sequence.
ACCESSION   DAZFXT010000035 DAZFXT010000000
VERSION     DAZFXT010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.035"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..234
                     /locus_tag="ACHKW0_001884"
     CDS             <1..234
                     /locus_tag="ACHKW0_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7474729.1"
                     /translation="IYRDKYGGTVMIKGVEERRVTYRREGYEYDCVMPVYQFDRDFSL
                     VQAVPRNVPTSREKARANIQEIRKMLNVFRGKK"
     gene            231..1985
                     /locus_tag="ACHKW0_001885"
     CDS             231..1985
                     /locus_tag="ACHKW0_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFO7474730.1"
                     /translation="MKLAPNVKQQSRGIKHKETEVIIFAGSDAWSHAKQWQEHDARMA
                     GDNEPPVWLGDQQLSELDKLQIVPEGRKSVRIFRAGHLEPVMIKAIGQKLAAAGVQDA
                     NFYPEGMHVQEVENWREYLARERQNLSEGLFVELPVKQKAQLSQMADSERAQLLSDRF
                     DGVCVHPESEIVHVWRGGLWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVI
                     AEPMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPN
                     FHKWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLA
                     GKQNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKY
                     EKRFTAVIRAVVLTTNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDKDLPEKVAAE
                     IPVIIRRLLANFADPEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLM
                     MGGGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVADFSKAMKPAAKVYGHEYITRKV
                     KGVTQTNAITTDDCDAFL"
     gene            2337..2582
                     /locus_tag="ACHKW0_001886"
     CDS             2337..2582
                     /locus_tag="ACHKW0_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474731.1"
                     /translation="MTKMRRDRTQQKYKALDMTELSLKVAIKVIDRHTREGYAKENPE
                     LISAFMTTAAANFATLTEREIAEAEQVTTINVKSTGE"
     gene            2713..2907
                     /locus_tag="ACHKW0_001887"
     CDS             2713..2907
                     /locus_tag="ACHKW0_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474732.1"
                     /translation="MEEIKQSATVMIDHEILNELKKLQFDVIRRMNEAGIYGRAATPT
                     IGHLARVLLRERLGIATGEA"
     gene            2911..3072
                     /locus_tag="ACHKW0_001888"
     CDS             2911..3072
                     /locus_tag="ACHKW0_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein NusG"
                     /protein_id="HFO7474733.1"
                     /translation="MAITEREASIIKAIGEETRDTIAPLMEKIAELETRINKLSNEVE
                     GLISLLGEQ"
     gene            3202..3690
                     /locus_tag="ACHKW0_001889"
     CDS             3202..3690
                     /locus_tag="ACHKW0_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051276 - chromosome organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7474734.1"
                     /translation="MALTTKKKRFADALSRGANPTEAAIHAGYSEKTARVKGWQLSND
                     PEVKKYLKAVTSDVTFQVTKPKKVTKKVTEKVTPERASINTIEMMDNGLPDPIKAMGR
                     ILIDNMDTDPKLALDAAFKLAQFTHRKIGSIGKKEVKKLNAASVYSNRFPIPKPPTDL
                     KH"
     gene            3702..3863
                     /locus_tag="ACHKW0_001890"
     CDS             3702..3863
                     /locus_tag="ACHKW0_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474735.1"
                     /translation="MSEYITDPIDVLTEIMNDKQQSTEIRLQAAAVLMPYFHETLNPC
                     FQEEEEDDL"
     gene            3853..4776
                     /locus_tag="ACHKW0_001891"
     CDS             3853..4776
                     /locus_tag="ACHKW0_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7474736.1"
                     /translation="MICKNMKGVSAVRAASVLASCKFSHADAIEMTKRNDPAVSKFLE
                     KSIVNRMTTDNMPSLVAEPIVRELGEYIFSQSVPGKLISSALNLPFGAKLHSISGKGS
                     KWLKEAEEIPVKEGEVNESALKLCKLGAIVMLNNELIKFSTPGTNSAIRDLITSESVK
                     EIDKKFLSSDQEVAGVSPEGVLNGADEAENFIEIFKKHIANGNTLSTSSLILPVENAL
                     QLTDAEFWQIELLRIPVIVSEQADRMMLIDAQKLIINVEATVITPTDEAVIKTESGAV
                     SLFQNDATAFRAITYCGWQKLDKAVTVLAKS"
     gene            5755..6075
                     /gene="clpS"
                     /locus_tag="ACHKW0_001892"
     CDS             5755..6075
                     /gene="clpS"
                     /locus_tag="ACHKW0_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HFO7474737.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            6106..8382
                     /gene="clpA"
                     /locus_tag="ACHKW0_001893"
     CDS             6106..8382
                     /gene="clpA"
                     /locus_tag="ACHKW0_001893"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HFO7474738.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(8726..8813)
                     /locus_tag="ACHKW0_001894"
     tRNA            complement(8726..8813)
                     /locus_tag="ACHKW0_001894"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8777..8779),aa:Ser,seq:gga)
     gene            complement(9067..9285)
                     /gene="infA"
                     /locus_tag="ACHKW0_001895"
     CDS             complement(9067..9285)
                     /gene="infA"
                     /locus_tag="ACHKW0_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HFO7474739.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(9570..10274)
                     /gene="aat"
                     /locus_tag="ACHKW0_001896"
     CDS             complement(9570..10274)
                     /gene="aat"
                     /locus_tag="ACHKW0_001896"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HFO7474740.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCSESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(10316..12037)
                     /gene="cydC"
                     /locus_tag="ACHKW0_001897"
     CDS             complement(10316..12037)
                     /gene="cydC"
                     /locus_tag="ACHKW0_001897"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HFO7474741.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALAESL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(12038..13804)
                     /gene="cydD"
                     /locus_tag="ACHKW0_001898"
     CDS             complement(12038..13804)
                     /gene="cydD"
                     /locus_tag="ACHKW0_001898"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0042883 - cysteine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HFO7474742.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(13927..14892)
                     /gene="trxB"
                     /locus_tag="ACHKW0_001899"
     CDS             complement(13927..14892)
                     /gene="trxB"
                     /locus_tag="ACHKW0_001899"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HFO7474743.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            15437..15931
                     /gene="lrp"
                     /locus_tag="ACHKW0_001900"
     CDS             15437..15931
                     /gene="lrp"
                     /locus_tag="ACHKW0_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HFO7474744.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            16066..20133
                     /gene="ftsK"
                     /locus_tag="ACHKW0_001901"
     CDS             16066..20133
                     /gene="ftsK"
                     /locus_tag="ACHKW0_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HFO7474745.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            20288..20899
                     /gene="lolA"
                     /locus_tag="ACHKW0_001902"
     CDS             20288..20899
                     /gene="lolA"
                     /locus_tag="ACHKW0_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HFO7474746.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            20910..22253
                     /gene="rarA"
                     /locus_tag="ACHKW0_001903"
                     /gene_synonym="mgsA"
     CDS             20910..22253
                     /gene="rarA"
                     /locus_tag="ACHKW0_001903"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HFO7474747.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            22344..23636
                     /gene="serS"
                     /locus_tag="ACHKW0_001904"
     CDS             22344..23636
                     /gene="serS"
                     /locus_tag="ACHKW0_001904"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HFO7474748.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            23875..26319
                     /gene="dmsA"
                     /locus_tag="ACHKW0_001905"
     CDS             23875..26319
                     /gene="dmsA"
                     /locus_tag="ACHKW0_001905"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009389 - dimethyl sulfoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HFO7474749.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            26330..26947
                     /gene="dmsB"
                     /locus_tag="ACHKW0_001906"
     CDS             26330..26947
                     /gene="dmsB"
                     /locus_tag="ACHKW0_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase iron-sulfur subunit
                     DmsB"
                     /protein_id="HFO7474750.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            26949..27812
                     /gene="dmsC"
                     /locus_tag="ACHKW0_001907"
     CDS             26949..27812
                     /gene="dmsC"
                     /locus_tag="ACHKW0_001907"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HFO7474751.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(27848..28474)
                     /gene="ycaC"
                     /locus_tag="ACHKW0_001908"
     CDS             complement(27848..28474)
                     /gene="ycaC"
                     /locus_tag="ACHKW0_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate family cysteine hydrolase YcaC"
                     /protein_id="HFO7474752.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            28789..29937
                     /gene="ycaD"
                     /locus_tag="ACHKW0_001909"
     CDS             28789..29937
                     /gene="ycaD"
                     /locus_tag="ACHKW0_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474753.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITSMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            30147..31577
                     /gene="ycaM"
                     /locus_tag="ACHKW0_001910"
     CDS             30147..31577
                     /gene="ycaM"
                     /locus_tag="ACHKW0_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7474754.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(31578..32486)
                     /gene="ycaN"
                     /locus_tag="ACHKW0_001911"
     CDS             complement(31578..32486)
                     /gene="ycaN"
                     /locus_tag="ACHKW0_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7474755.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERSGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            32586..33176
                     /gene="ycaK"
                     /locus_tag="ACHKW0_001912"
     CDS             32586..33176
                     /gene="ycaK"
                     /locus_tag="ACHKW0_001912"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HFO7474756.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGQELHASYYQSLLSQVRDMVDAL"
     gene            complement(33258..33998)
                     /gene="pflA"
                     /locus_tag="ACHKW0_001913"
     CDS             complement(33258..33998)
                     /gene="pflA"
                     /locus_tag="ACHKW0_001913"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043365 -
                     [formate-C-acetyltransferase]-activating enzyme activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HFO7474757.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(34190..36472)
                     /gene="pflB"
                     /locus_tag="ACHKW0_001914"
     CDS             complement(34190..36472)
                     /gene="pflB"
                     /locus_tag="ACHKW0_001914"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008861 - formate C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HFO7474758.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(36527..37384)
                     /gene="focA"
                     /locus_tag="ACHKW0_001915"
     CDS             complement(36527..37384)
                     /gene="focA"
                     /locus_tag="ACHKW0_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HFO7474759.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT         9685 a         9036 c         9871 g         9027 t
ORIGIN      
        1 atttaccgcg ataagtacgg cggcacggta atgattaagg gcgtggagga acggcgcgta
       61 acttaccgcc gtgaaggcta cgaatatgat tgcgttatgc ctgtttatca gttcgaccgt
      121 gatttttctc tggtgcaggc tgtaccgcgt aacgtgccca ccagcaggga gaaagcacgc
      181 gccaatattc aggaaataag aaagatgttg aacgtattca ggggtaaaaa atgaaacttg
      241 caccgaacgt aaaacagcag tcacgcggca taaaacacaa agaaacagaa gtcattattt
      301 ttgcgggtag tgatgcctgg tcacacgcta aacaatggca ggaacatgac gcgcgtatgg
      361 ccggagataa tgagcctcct gtgtggcttg gggatcagca gttatccgaa ctggataagc
      421 tgcaaattgt gccggaaggc cgaaaatcag tacgcatatt cagggccgga catcttgaac
      481 ctgtaatgat aaaggcgatt ggtcagaagc tggcggcggc aggtgtacag gatgcaaatt
      541 tttaccctga gggaatgcac gttcaggagg tggagaactg gcgcgaatat ctggcccgtg
      601 agcgccagaa tctttctgaa ggtctgttcg ttgagcttcc ggtaaagcaa aaggcgcaac
      661 tttcgcagat ggcggacagt gaacgcgctc agctgctttc cgatcgcttt gatggcgttt
      721 gcgtacatcc tgaaagtgaa atcgttcacg tatggcgcgg cgggttatgg tgtccggtca
      781 gcacaatgga acttagccgc gaaatggtgg cgatctattc agagcacagg gccactttca
      841 gcaagcgcgt aatcaataac gccgttgaag cgttaaaagt tattgccgaa ccaatgggcg
      901 agccgtccgg cgatttgttg ccgttcgcca atggtgcgct tgacctgaaa acgggggaat
      961 tttccccgca cacgccggag aactggatca ccacgcacaa cggtattgag tacacgccac
     1021 cagcaccagg ggagaacatc cgcgataacg cgccaaattt tcataaatgg cttgagcacg
     1081 cagccggaaa agacccgcgc aagatgatgc gtatatgtgc cgcgctgtac atgattatgg
     1141 cgaaccggta cgactggcag atgtttattg aggccaccgg agacggcgga agcggtaaaa
     1201 gtacattcac gcacatagcc agccttctgg cagggaaaca gaacacggta agcgctgaaa
     1261 tgacatcgct tgatgatgct ggtggacgtg cgcaggttgt cgggagtcgt cttatcgtcc
     1321 tggcagacca gccgaaatat acaggcgaag gaacgggcat caagaaaatc acgggcggcg
     1381 accccgtgga aattaacccg aaatatgaaa agcgtttcac ggcggtaatc agggcggtgg
     1441 tgctgacgac caacaacaac ccgatgatat tcaccgaacg ggccggaggc gtggcacgtc
     1501 gtcgggttat ttttcgcttt gacaatatcg ttagcgaggc agaaaaggac aaggatttgc
     1561 cggagaaggt cgcggctgaa atccctgtga ttatccgccg cttgctggct aattttgctg
     1621 accctgaaaa ggcacgggct ttattactgg aacagcgcga cggtgatgaa gcactggcga
     1681 taaagcaaca gacagatccg gttattgagt tttgccagtt cctgaatttt ctggaggaag
     1741 cgcgcggcct gatgatgggt ggcggtggcg attccgtgaa gtacacgacc agaaacagcc
     1801 tttaccgcgt ctatctggcg tttatggcat acgcaggaag aagcaagccg ctaaacgtgg
     1861 ctgatttcag caaggccatg aagccagcgg cgaaagttta cggacatgaa tatattacgc
     1921 gaaaagttaa gggagtaacg cagaccaacg caattacaac ggatgattgt gacgcgtttt
     1981 tataattttt tgtaaaagcc ctctacccca tctacctgaa tgaaataaac gcatattatt
     2041 caacatgata agtgggtaga gggataggta gaaggctaat aaaacccctc taccccctct
     2101 accatgatta aggtcgtttg tgaggggcgg gtagaggatg ggtagagggc cacgaaaagt
     2161 cctctaccca tctgaaagcc gcgccattac tgacctgaaa gatgattagg tagaagaggt
     2221 agaggaggtg caccacaacc taaaactttt taaacgaggg ggggtaaaaa taaatgtgca
     2281 tacatcaaaa tcacttaaca aacatgccga ccgaaaacat gaatcagggg cgacaaatga
     2341 ccaaaatgcg cagagacaga acacagcaaa aatataaagc gttagacatg acagagcttt
     2401 ccctaaaggt ggcaatcaaa gttatagacc gccacacacg ggaaggatac gcgaaggaga
     2461 atcccgaact aataagcgca ttcatgacca cggcggcggc aaactttgcc acgctgacag
     2521 agcgggagat tgcagaagcg gaacaggtaa caaccatcaa cgttaaaagc actggagaat
     2581 aaccgaagga gtgagcacat agccggagca atccggcttt ttttcgatct ctcatatctt
     2641 aaaaaatggt tgattaatga taatctatcg aaaatactga ttatgcgatg ataagtatca
     2701 ggaggaaaca aagtggaaga gataaagcaa agcgctaccg tgatgattga tcacgagatc
     2761 ctaaacgagc ttaaaaaact tcaattcgat gttattcgcc ggatgaatga agctggtata
     2821 tacggcagag ccgccacgcc aacaataggt catcttgcac gtgtgctgtt acgcgaaaga
     2881 ctgggcattg caacaggtga ggcataacaa atggctatca cagaacgcga ggcaagcatc
     2941 attaaggcca ttggcgagga aacaagagac accatagcgc cattgatgga aaagatcgca
     3001 gagcttgaga cacgaataaa caagctgagt aatgaagttg aaggcctgat ctcgttactc
     3061 ggtgagcaat gacatggggg gggagtgttc cggccggaaa gtcgatcacc ctggacaccg
     3121 ccccccgtct cattcgtaga aaattttgct tttcaaaaaa gttacacgaa aagttacacc
     3181 ccctcaaaac gaggtttttg catggcatta acgaccaaaa aaaagcggtt tgctgatgcg
     3241 ttatcaaggg gcgcaaatcc cacggaagcc gctatacatg cgggatacag cgaaaagacc
     3301 gcaagggtta aaggctggca gctatccaac gatcctgagg tgaaaaagta tcttaaggct
     3361 gtaacttctg atgtaacttt tcaggttaca aaaccgaaaa aagttacaaa gaaagttacc
     3421 gaaaaagtta caccggaacg ggcatcaatt aacaccattg agatgatgga taacggccta
     3481 cccgacccca tcaaagcaat gggcaggata ctgattgaca acatggacac agatccaaaa
     3541 ctggctttag atgccgcgtt taagctggcc cagttcaccc accgcaagat cggcagcatt
     3601 gggaaaaaag aggttaaaaa actcaatgcc gctagtgtct actcaaatcg gttccccatc
     3661 cctaagccac caacagactt aaaacactga ggtagcgaac tatgagcgaa tacatcacag
     3721 accctattga tgtactcacg gaaatcatga acgacaagca gcagtcgacc gaaatacggt
     3781 tacaggccgc cgctgttctg atgccatatt ttcacgaaac actaaacccg tgcttccagg
     3841 aggaggaaga agatgatctg taaaaacatg aaaggcgtgt cagcagttcg cgccgccagc
     3901 gtgctggcat cgtgtaaatt tagccatgct gacgctatag agatgacgaa aagaaatgat
     3961 ccggcagtat cgaaatttct tgaaaaatcc atcgtcaaca gaatgactac ggataacatg
     4021 ccgtcgctgg tggcagagcc gatagtaaga gagcttggcg agtatatttt ttcgcagtct
     4081 gtaccaggca agctaatcag cagcgcgctt aatcttccgt ttggcgcaaa attgcattca
     4141 atatcaggca agggtagtaa atggcttaaa gaagccgaag aaatccctgt caaagaggga
     4201 gaagtaaacg aaagtgcgct aaaattgtgc aaacttggcg ctattgtgat gctgaataac
     4261 gagctaatca aatttagcac ccctggtaca aattcagcga tcagagactt gatcacatcg
     4321 gaatcggtga aagaaataga taaaaaattt ctttccagtg atcaggaggt ggctggcgta
     4381 tcccctgagg gcgtgcttaa tggtgctgat gaggccgaaa acttcataga gattttcaaa
     4441 aagcacatag cgaacggtaa cacgctttcc acgtcgtcgc taatattgcc cgtagaaaac
     4501 gccttacagc ttactgatgc tgagttctgg cagatagagc ttttgagaat accggtaatt
     4561 gtgagcgaac aggcggatcg catgatgctg atagacgctc aaaagctgat tattaacgtt
     4621 gaagccacgg ttataacccc aactgatgag gcggtaataa aaaccgaatc aggggcggtt
     4681 agccttttcc agaatgacgc tacagcgttc agggcaatca cctattgcgg atggcaaaaa
     4741 ctcgataagg ccgtcaccgt tctggcgaaa agctgatttt tccagcatct atcccgatat
     4801 ggggatcacc atatcgggga agcacctgaa tcatcattac cgtgatcaag ggtatgtaaa
     4861 gcccaccagc ctgattaacg tttaaccgga aaaaaagcca ggtatccaat ctcgatatgg
     4921 ggatcaccat atcgggatac ggcggtaact ggttgatatt ttcgaaattc gccagcggcg
     4981 aacatcgggg aaaccagcct aacccgttga ttcttcccaa tttccacaga ctgcggaaat
     5041 tggcgcggta acttactgag attaaagcaa agcgcaaaat tgcgttggct ggtgggcctg
     5101 atgccgataa ctggttggaa ttgttccata gtgcaaaatt gcactatggc ctaacccatt
     5161 gattatttcg aaaaccccca ttgggggatg ttgggaaagt aagactaacc cattgattct
     5221 tccacaatcc tcaatttgag gagatcggaa acatcaccct tcaaattctg aatggtcata
     5281 atgaccacgc agaccatcaa ggcaaccaca acgaacatcg ttacggttgg agcttttacc
     5341 ccactgggga aaaggtacgc acctgaatca tcattaccgt gatcaagggt atgtaaaggc
     5401 caccagcctg attaacattt aaccggaaaa aaagccaggt aggtggggat acccatatcg
     5461 gggaacatcc accagcttca taacggatgg ccggaaaatg ataacggtta aagcgtgttg
     5521 atctttgttc gtggttgttc gatattgttc gtgaaacggt gtagttaatg gtgtagttaa
     5581 tttcgctgtt tttgacggag ttgttatttg taacatagtg aaaaataaag actaatcaat
     5641 gaaatgttat aaatgatagt tatctatcat gtggagtaga ttggtcaggc aaataagctc
     5701 ttgtcagcgg cagggcgttc tgccgataac cgtaaccgaa gatgataact gacaatgggt
     5761 aaaacgaacg actggctgga ctttgatcaa ctggcggaag aaaaagttcg cgacgcgcta
     5821 aaaccgccat ctatgtataa agtgatatta gtcaatgatg attacactcc gatggagttt
     5881 gttattgacg tgttacaaaa attcttttct tatgatgtag aacgtgcaac gcaattgatg
     5941 ctcgctgttc actaccaggg gaaggccatt tgcggagtct ttaccgccga ggttgcagaa
     6001 accaaagtgg cgatggtgaa caagtacgcg agggagaatg agcatccatt gctgtgtacg
     6061 ctagaaaaag cctgaatgca ggcataaaaa ttgggggagg tgcctatgct caatcaagaa
     6121 ctggaactca gtttaaatat ggctttcgcc agagcgcgcg agcaccgtca tgagtttatg
     6181 accgtcgagc acttgttact ggcgctgctc agtaacccat ctgcccggga ggcgctggaa
     6241 gcgtgttctg tggatttggt tgcgctccgt caggaactgg aagcctttat tgaacaaacc
     6301 acacccgttc tgcctgccag tgaagaggag cgcgacacac agccgacgct gagttttcag
     6361 cgtgtactgc aacgtgcggt cttccatgtc caatcctccg gtcgcaatga ggtaaccggt
     6421 gcaaacgttc tggtcgctat ctttagcgaa caggagtcgc aggcggcata tctgttgcgt
     6481 aaacatgaag tcagccgtct cgatgtggtg aactttatct ctcatggcac gcgtaaagac
     6541 gagccgacac agtcttctga tcctggcagc cagccaaaca gcgaagaaca agctggtggg
     6601 gaggaacgta tggagaattt cacgacgaac ctgaatcagc ttgcgcgcgt gggcggaatc
     6661 gacccactga ttggtcgtga gaaggagctg gagcgtgcta ttcaggttct ctgccgtcgc
     6721 cgtaaaaaca acccgctgct ggtgggggaa tctggtgtcg gtaaaaccgc gattgcggaa
     6781 ggtcttgcct ggcgaattgt tcagggcgat gtgccggaag tgatggctga ctgtacgatt
     6841 tactctctcg atatcggttc tctgttagcg ggcactaaat atcgcggcga ctttgaaaaa
     6901 cgttttaaag cgttgctcaa gcagctggag caggacacta acagcatcct gtttattgat
     6961 gagatccaca ccattatcgg tgcgggggca gcgtctggtg gccaggtcga tgcggctaac
     7021 ctgatcaaac cgttgctctc cagcggtaaa attcgcgtaa ttggttcgac aacctatcag
     7081 gagttcagca acattttcga gaaagaccgt gctctggcgc gtcgcttcca gaaaattgat
     7141 attactgaac cgtcgatcga agaaactgtt caaatcatca atggcctgaa accgaagtat
     7201 gaagcgcacc acgacgtgcg ttataccgca aaagcggtgc gtgcagcggt agagctggcg
     7261 gtgaaataca ttaacgatcg tcatctgccg gataaagcca ttgacgttat cgacgaagcg
     7321 ggcgctcgcg cacgcctgat gccggtaagc aaacgcaaga aaaccgttaa tgtggcggat
     7381 attgagtccg tggtggcccg tattgcgcgc attccagaga agagtgtttc tcagagtgac
     7441 cgcgataccc tgaaaaacct cggcgatcgc ctgaaaatgc tggtcttcgg tcaggataaa
     7501 gccattgagg cgctgactga agccattaag atggcgcgtg caggtttagg tcacgaacat
     7561 aaaccggttg gttcgttcct gtttgccggc cctaccgggg tcgggaaaac agaggtgacg
     7621 gtacagcttt cgaaagcgtt gggcattgag ctgctgcgct ttgatatgtc cgagtatatg
     7681 gaacgccata ccgtcagccg tctgattggt gcgcctccgg gatacgttgg ttttgatcag
     7741 ggaggtttgc tgactgatgc ggtcatcaag catccacatg cggtgctgtt gctggacgaa
     7801 atcgagaaag cgcatccgga cgtgttcaat attctgttgc aggtgatgga caacggtacg
     7861 ctgaccgata acaacggacg caaagcggac ttccgtaacg tggtgctggt gatgaccacc
     7921 aacgccgggg tacgtgaaac tgagcgtaaa tccattggtc ttatccacca ggataacagc
     7981 accgatgcga tggaggagat caagaagatc tttacaccgg aattccgtaa ccgtctcgac
     8041 aacattatct ggtttgatca tctgtcaacc gacgtgatcc atcaggtggt ggataaattc
     8101 atcgtcgagt tgcaggttca gctggatcag aaaggtgttt ctctggaagt gagccaggaa
     8161 gcgcgtaact ggctggccga gaaaggttac gaccgggcaa tgggcgcacg tccgatggcg
     8221 cgtgtcatcc aggacaacct gaaaaaaccg ctcgccaacg aactgctgtt tggttcgctg
     8281 gtggacggcg gtcaggtcac cgtcgcgctg gataaagaga aaaatgagct gacttacgga
     8341 ttccagagtg cacaaaagca caaggcggaa gcagcgcatt aatctgattg ttaggtaggt
     8401 tggtcaagtc cgtaatctcg aaagaggtta cggacttttt gtttatgggg tggaggaggt
     8461 tcagaccctt tttttaatga tgatggtaag ttgttgataa ttagtgctgc gggaaggtaa
     8521 ggataaaaaa gggtgctgca ggagaatggg atggttttgc tttattaaca acgggctaaa
     8581 cgtgtagtat ttgagttcac tgccatacag gcagcttaga aatcgacggg gtgcggtaaa
     8641 acctttgcga acgcgttcac tgccgtacag acagataaaa tgcgaaaaaa aagctcgcac
     8701 tttcgtacga gctcttcttt aaatatggcg gtgagggggg gattcgaacc cccgatacgt
     8761 tgccgtatac acactttcca ggcgtgctcc ttcagccact cggacacctc accaaattgt
     8821 tttgtcgcct gacctcatgg gtggcaacgg ggcgctacta tagggagttg gagtaaaacg
     8881 gtcaagaaga attttaatga taattattgt ttgctcatac tgtaaacaag ttgtgcagta
     8941 tatctacatc gagacaagtt acggacttat acttccaaag tacttcatac atatcacaaa
     9001 ataaaaaggc cggttaaacc gaccttttac tcgttctttc tcttcgccca tcaggcggta
     9061 aaacaatcag cgactacgga agacaatgcg gcctttgctc aggtcgtacg gggtcagttc
     9121 aacagtcact ttgtcgcccg tcaggatgcg gatgtagttt ttgcgcattt taccggagat
     9181 gtgtgcagta accacgtgac cgttttctaa ctctacgcgg aacatggtat taggcaacgt
     9241 ttcaagaacg gtaccttgca tttcaatatt gtcttctttg gccatctaat cctctggggt
     9301 atcactaccg taatttgaac cggcaagata atgccgaagt tctgtaaata agtaaagatt
     9361 tgcgcgctaa atcgcaacaa acaggttcgg cacattactc cgaaaacaca cggctaagcc
     9421 gcaccaaaag cgcaacgtat aagggagcgg tgagataaac gatgggcgtt acctgacgcg
     9481 aaaaattcct tatcggcagc ggggtaatga gcgtaaccaa ctctgcgacc gcaattataa
     9541 cactctgggg agaaatgtgc cgaaaacatt cattcttgtg gtgaaaacaa gcatcgtggt
     9601 acccagaaat tattcggcaa tcgtccgagg cgcatttgat tgagataatt aaggtaatcc
     9661 cggcggggaa tttcgcaggc accaagcgat gctgtgtgat cgttaaggac ctggcagtcg
     9721 ataagcttac cgccatgacc gataaattcc tcacagaata ccagaagcgc cgttttagac
     9781 gcattttcca tccggctgaa catggactcg ctacaaaata gcgttccctg ggccacgccg
     9841 tacataccgc cgacaagctc atcttcacgc cagacttcaa tggagtgggc atggccgagt
     9901 tcgtgaaggc gatggtaggc ttcgaccacg ccacgcgtga tccaggttcc ttcttcgcga
     9961 tcgctggcac agccttcaat gacctgacca aaagcgtaat tcatcgtgac acgatagggc
    10021 gagcgtttat gaaatcgctt catactacgg ctgatatgca gtgattctgg ccatagcacc
    10081 gcgcggggat cgggcgacca ccagaggatg gggtcgcctg gagaaaacca cggaaaaata
    10141 ccacgctggt aagccattaa caggcgcgca gggctaagat cgcccccaag tgccagcagg
    10201 ccgttaggct cacgtaatgc gccttccggg gaagggaagg ctattgaatg gcgagaaagc
    10261 tgaaccaggc gcatgaccgc aaaactccac gcaagtcgga tcgttcaata atagcttaca
    10321 aaccctgctt gaactggtaa taacgcccct gtctggcaag cagctctgcg tgagtacctt
    10381 gctcaataat ttgcccgttg tccatcacta ttatttgttg gaaacgagag agtccgcgaa
    10441 gtcgatgggt gaccattaac accgttttct cacgcatcat ttctgcaagc aattcaagga
    10501 tctggctttc ggttgtggca tctaagcctt cggtaggttc atccagcaac accagtggcg
    10561 catcatgtaa cagcgcacgg gcgatagcca gacggcgcag ttcaccaccg gagagctggc
    10621 gtccgccttc acctaaccaa ctgttgagac ctgcatcctc gagcagcttc tccaggccaa
    10681 cgcgacgcaa gctctccgcc agagcctcat cactactgcc aggcgaggcg agtaacagat
    10741 tatcacgcag cgtggcgcta aacagatgca ctcgctgagg aacaacgctg atggtctgtc
    10801 gtagagccgc ttcattcagg ctggctatgg ggctatcgtt aagcaaaatc tcgccctgtt
    10861 gcgggtccca agcgcgggtc agcagttgta acagtgttga tttgccgcat ccggttcgcc
    10921 cgagaatcgc aatatgttcc ccggcgttta cctgaagaga aatcccttta agtgcctgtt
    10981 gggattgctc cggataagtg aactgaacat cccgtaatgt cagcgaaacg cgatcggcaa
    11041 cacgagtttg ggtatcaggg aaggtgactt ccggtttttg atccgttaaa tcagtgatcc
    11101 gaacggcaga ggcaatgact tgccccagat gctgaaatgc acccgttact ggtgccagtg
    11161 cttcaaacgc ggctaacgcg cagaagacaa acagggcaat taacgcgccg ggttgagcat
    11221 tgccgccaac gccgccagac gccatccaca gcatcaggat caccgctaac gcgccaatga
    11281 gtagcattat cgcttgcgac aatgcggtca gttcagattg acggcgttgc gcttccagcc
    11341 attgaatttc tgtattctct agttgcgtgc gataacgatc gctggcacca aaaatggtca
    11401 gctcagcttg cccttgcagc caggccgtca gttgttggcg atactgtccg cgaagatgag
    11461 tcagattttg cccggtgctt tttcccgcac gataaaacag cggtggcatc aggaaaagcg
    11521 tcagtaacat aatgccgccc agcgtaaagg cgagggtgaa atcaaggaaa cttaacccga
    11581 ttgtcaccac cataatcacc acaaaagcgc ccaccagcgg cgagataacg cgcagataaa
    11641 gatgatcgag cgtatcaaca tccgccacca cgcgattgag caattcgccc tgacgatagc
    11701 gcgccagtcc ggcaggggag aggggcagca atttgctgaa ggtgtaaatg cgcagatgct
    11761 gcaacacgcg gaaagtcgcg tcgtgactta ccagacgttc aaaataacgc ccggcagtac
    11821 gggtgattgc tgcgccacgc acgcccgcag cgggtagcat atagttgaag ctgtacagtc
    11881 cggcaacccc cgcaaccgct gaggccgaga ggaaccagcc ggaaagtgtc aacagaccga
    11941 tactggcgag cagcgtgaca attgccagca cgataccaag acttaacatc catttatgac
    12001 gtttatacag tgccagatag ggtagcaaag cgcgcattta aatctcctcc tgacgatggg
    12061 ccagtaatgt ggcgaatggg ccaccagcca cacttaattc cgcgtaacgt ccttgctcaa
    12121 taatccggcc atcctgcata acccaaatga catcccagtc agcaagatct tctaactggt
    12181 gggtgaccat taacgttgtc tggcgcagag aggcggcatt cagcgcctcc attacgcgct
    12241 gttcactgtg agcatcaagg ctggcagcgg gttcatccaa cagtaatagc gaacagggat
    12301 ttagtaacgc acgggccacc gccacgcgct gcgcctgccc cacggaaagg cgggcagcct
    12361 ggtcgccaac aggcgtatca acgccttgtg gcaggagcgg tagaaactcg ctgacccagg
    12421 cgttatccag cgctgcttgt aattcttgtt cgctggcatc aggtcgcgcc agtagtacgt
    12481 tatcccgcaa tgttgctgcc ggtaattgtg ggttttgccc aacccaggag agatgtttac
    12541 gccatgattc tggtgataaa tcgcgtaatt ctatcccgtt gattcgtaat gatccctgat
    12601 atgaaagaaa accagaaagc gcgttcagca gtgagctttt acctgaaccg ctgcgaccaa
    12661 ccaacactgc acgttggcct gctggcaaag taaagttcag cggtccggcc agcgttttac
    12721 cttccggcga cgtgataaac agatcctcgg cctcaatggt caccggatcg gtcgatgcta
    12781 attccgcctc accgcgctgc ggatgggcga gcggggtttc cataaacgtt ttcagactgt
    12841 cagctgcacc aacagcctgg gctttagcat gataaaacgt accgagatcg cgcaatggct
    12901 ggaaaaactc tggcgcaagg atcagggcca gaaaacccgc agccagcgtc acaccggtat
    12961 cgtagtgacc aaaatccagc tcgccgagat aggaaaaacc aaagtagacc gccaccagag
    13021 caattgacag cgaggtaaaa aattcgagaa tgccggagga taaaaacgcc agccgtagca
    13081 cttccattgt ccgttggcgg aaatcttccg aagcagaacg aatactttca atttcagctt
    13141 caccacgacc aaaaatacgc aatgtttcca tgccgcgcag gcgatcgagg aaatgcccac
    13201 ttaagcgagc aagagcgaga aagttacgcc ggttagcatc ggcagccccc atcccaacca
    13261 gcgccataaa caacggaatt aacggtgctg tgcccagcag aatgagcgcc gcagcccagt
    13321 tagaggggaa gatcgccacc acaatcagca acggcaccga cactgccagc gccatttgcg
    13381 gcaggtagcg tgcatagtaa tcatgcatat cgtcaatttg ctcgagtacc aacgtcgccc
    13441 agctccccgc aggtttaccc tgaatccacg ctggccctgc ttgttgcaga cggtcgagaa
    13501 cctgacggcg gatggcaaag cggatatgct gcccggcgtg ataacccacc cgttcgcgta
    13561 accagaccac ccatgcgcgc agtacaaagg tcagaaccag taacgtaaag ggaagcagca
    13621 gggcttcacg gggaatattc tccataatca tatgttgcag aattcgcgcc atgaaccagg
    13681 cctgggcaat aatcaatatg ccgcttacaa agcccagcag acgagaaata ttcagccaac
    13741 gttgggagat gacgctttgc tgttttaacc agcgggttaa ctctttttga cgggatttat
    13801 tcattgcacg cttagcaggt gagttatcag aattatttgc agagcaatgt tacaacgggg
    13861 aaaaaataaa ggcgacccat agtcgcatgg tgtcgccttc tttacttttg ttactgattt
    13921 gtaaaattat tttgcgtcag ctaaaccatc gaggtagcgt tccgcatcaa gtgctgccat
    13981 gcagcctgta ccggccgaag taatggcctg acgataaata tgatccatca cgtcacccgc
    14041 ggcaaagacg ccagggatgc tggtctgggt ggcattacca tgaatacccg actgcacttt
    14101 gatgtagccg ttttccagtt ccagctgccc ttcgaaaatg gcagtattcg ggctgtgacc
    14161 gatagcaaca aacagaccag caacgtcgag tgactcgatg ttatcgctgt tttgcgtatc
    14221 gcgcagacga acgccagtga cacccatttg atcgccggtc acttcttcca gcgtacggtt
    14281 ggtgtgcaga atgatgttgc cgttctccac tttatccatc agacgcttaa taaggatttt
    14341 ttccgcgcgg aaaccgtcac ggcggtgaat cagatgcact tccgaagcga tgttagacag
    14401 atacagcgcc tcttcaaccg cggtattgcc gccgccgatg accgcaactt tctggttgcg
    14461 atagaagaaa ccgtcgcagg ttgcacaagc agaaacccca cggcctttaa aggcttcttc
    14521 agagggcagg ccgagatagc gtgcagaagc tccggtggca ataatcagcg cgtcgcaagt
    14581 gtattcgccg ttatcgccat tcagacggaa cggacggttt tgcagatcca ccttgttgat
    14641 atgatcaaaa atgatctcag tttcaaactt ggtggcatgt tcgtgcatgc gctccattaa
    14701 taacggaccg gtcagatcgt ttggatcgcc aggccagttt tccacttccg tggtggtggt
    14761 cagttggccg cctttttcca tgccggtaat cagcacaggt tgcaggttgg cgcgcgccgc
    14821 gtagacagca gcggtgtatc ccgccgggcc tgaacccagg ataagcagtt tactgtgttt
    14881 ggtcgtgccc atgagatccc catagttgtt ggcagacaat gggcaggatt gtagggaatt
    14941 tacagacgta aaaaaagagt atgacgattt tgttaacaat ttgtgcaatc ggcagcatcg
    15001 ataagcaggt caaattctcc cgtcattatc acctctgcta cttaaatttc ccgctttata
    15061 agccgattaa atgatgaata aacgcccctg ttaatgaata tctggcatgt tgtactaaaa
    15121 atcgatgttt tgctttgaca atcccctggt gttttgcgaa aacattcgag gaagaaaaaa
    15181 aacagtattc ttatatgcgc ataaccatgc atgtaaatac catgtttacc gtgctagtga
    15241 aatctacgta tggcgtggac agacgccatt cgtgatgtcg atagctgcca caaggcaacg
    15301 gtcttctcac cgtagaccca ggcattgcgc gccgtgaatc ttcatgattt cggtctatcg
    15361 tgacgggtag cgactctgaa cagtgatgtt tcagggtcag acaggagtag ggaaggaata
    15421 cagagagaca ataataatgg tagatagcaa gaagcgccct ggcaaagatc tcgaccgtat
    15481 cgatcgtaac attcttaatg agttgcaaaa ggatgggcgt atttctaacg tcgagctttc
    15541 taaacgtgtg ggactttccc caacgccgtg ccttgagcgt gtgcgtcggc tggaaagaca
    15601 agggtttatt cagggctata cggcgctgct taacccccat tatctggatg catcacttct
    15661 ggtattcgtt gagattactc tgaatcgtgg cgcaccggat gtgtttgaac aattcaatac
    15721 cgctgtacaa aaacttgaag aaattcagga gtgtcattta gtatctggtg atttcgacta
    15781 cctgttgaaa acacgcgtgc cggatatgtc agcttaccgt aagttgctgg gggaaaccct
    15841 gctgcgtctg cctggcgtca atgacacacg gacatacgtt gttatggaag aagtcaagca
    15901 gagtaatcgt ctggttatta agacgcgcta acacggaaca ggtgcaaaat cggcgtattt
    15961 tgattacact cctgttaatc catacagcaa cagtactggg gtaacctggt actgttgtcc
    16021 gttttagcat cgggcaggaa aagcctgtaa cctggagagc ctttcttgag ccaggaatac
    16081 actgaagaca aagaagtcac attgacaaag ttaagtagcg gccgtcgcct tctggaagcg
    16141 ttgctgatcc ttattgtcct gtttgccgtc tggttgatgg ctgccttact aagctttaac
    16201 ccttcggacc ccagctggtc gcaaacggcc tggcatgaac ctatccataa tttaggtggg
    16261 atgcccggtg cgtggctggc agatacgctg ttctttattt ttggcgtgat ggcttacacc
    16321 attcccgtca ttattgtcgg cggttgttgg tttgcctggc gtcatcagtc cagtgacgaa
    16381 tacattgatt attttgccgt ttcgctacgc atcattggcg ttttggcgct catccttacc
    16441 tcctgtggtc tggcggcaat caacgctgac gatatctggt attttgcctc cggtggcgtc
    16501 attggcagct tactaagcac tacgctacaa ccactgctac acagtagcgg gggaactatt
    16561 gcgctgctct gcgtttgggc tgcgggcctg acgctgttca ccggttggtc atgggtgacc
    16621 attgctgaaa aactcggcgg ctggatttta aacattctca ccttcgccag taatcgtacc
    16681 cgtcgcgatg atacctgggt cgatgaagat gaatatgaag atgacgaaga gtatgaagat
    16741 gaaaaccacg gcaaacagca tgaatcgcgc cgtgcccgta ttcttcgcgg cgcgctagcg
    16801 cgtcgtaaac ggttggcgga aaaattcatt aatccgatgg ggcggcaaac agacgctgcg
    16861 ttgttctccg gtaagcggat ggatgatgac gaagagatta tctacactgc acgcggtgtg
    16921 gctgccgacc cggacgacgt cctattttcg ggcaatcgtg caacgcagcc agaatatgac
    16981 gaatacgatc cattattaaa cggtgcgcca attaccgaac ctgtcgctgt agcagctgct
    17041 gctaccacgg cgacacaaag ctgggctgcg ccggttgaac ctgtgactca gacgccgcct
    17101 gttgcctctg ttgatgttcc accttcgcaa cctacagtag cctggcagcc tgtaccgggt
    17161 ccacaaacgg gagagccggt tattgctcct gcaccggaag gttacccaca gcagtcacaa
    17221 tatgcgcagc ctgcagtgca atataatgag ccgctgcaac aaccagtaca gccgcagcag
    17281 ccgtattatg cacctgcagc tgaacaacct gcgcaacaac cttactacgc tcctgcggcg
    17341 gaacaacctg ttcagcaacc gtattacgcc cctgcgccag aacaaccggt ggcaggtaac
    17401 gcctggcaag ccgaagagca gcaatccact tttgctccac agtctacata ccagactgag
    17461 caaacttatc agcaaccagc cgctcaggag ccgttgtacc aacagccgca atccgttgaa
    17521 cagcagcctg ttgtggagcc tgaacccgtt gtagaagaga caaaacccgc gcgtccgccg
    17581 ctttactatt ttgaagaagt ggaagagaag cgagcccgtg aacgtgaaca acttgcggcc
    17641 tggtatcaac cgattccaga accggttaaa gaaccagaac cgatcaaatc ttcgttgaaa
    17701 gcgccttctg ttgcagcagt acctccagta gaagccgctg ccgctgtttc tccgctggca
    17761 tctggcgtga aaaaagcgac actggcgacg ggggctgccg caaccgttgc cgcgccagtc
    17821 ttcagtctgg caaatagcgg tggaccgcgt ccgcaagtca aagaggggat tggcccgcag
    17881 ttgccacgac cgaaacgtat ccgcgtgcca actcgtcgtg aactggcgtc ttacggtatt
    17941 aagctgccct cacagcgtgc ggcggaagag aaagcccgtg aagcccagcg caatcagtat
    18001 gattctggcg atcagtataa cgatgatgaa atcgatgcga tgcagcagga tgaactggca
    18061 cgtcagttcg cccagacaca gcagcaacgc tatggcgaac agtatcaaca tgatgtgccc
    18121 gtaaacgcag aagatgcaga tgctgcggca gaggctgaac tggctcgtca gtttgcgcaa
    18181 actcaacaac aacgttattc cggcgaacaa ccggctgggg cgaatccgtt ctcgctggat
    18241 gattttgaat tttcgccaat gaaagcgttg ctggatgatg gtccacacga accgttgttt
    18301 acgccaattg ttgaacctgt acagcagccg caacaaccgg ttgcaccgca gcagcagtat
    18361 cagcagccgc aacaaccagt tccgccacag cagcaatatc agcagccgca acagccggtt
    18421 gcgccgcagc cgcagtatca gcagccacaa cagcaggttg cgccgcagcc gcagtatcag
    18481 cagccacaac aaccggttgc gccacagccg caatatcagc agccgcaaca accagttgcg
    18541 ccgcagccgc agtatcaaca gccgcaacaa ccggttgcac cgcagcagca ggataccctg
    18601 cttcatccgc tgttgatgcg taatggcgac agccgtccgt tgcataaacc gacgacgccg
    18661 ctgccttctc tggatttgct gacaccgccg ccgagcgaag tggagccggt agataccttt
    18721 gcgcttgaac aaatggctcg cctggtggaa gcgcgtctgg ctgatttccg tattaaagcc
    18781 gatgtcgtca attactctcc ggggccggtt atcactcgct ttgaacttaa cctggctcct
    18841 ggcgtaaaag cggcgcgcat ttctaacttg tcacgggacc ttgcccgttc actttcgacg
    18901 gtggcggtgc gtgtcgttga agttattcct ggcaaaccct atgtaggtct ggagttacca
    18961 aataaaaaac gacaaaccgt ttatctgcgc gaagttttgg ataacgccaa attccgcgat
    19021 aatccgtcgc cattaaccgt ggtgctgggt aaagatatcg ccggtgagcc ggtggttgcc
    19081 gatctggcga aaatgccgca cttgttggtt gcggggacta ccggttccgg taaatctgtc
    19141 ggtgtgaacg cgatgatcct gagcatgctt tataaagcac agccagaaga tgtgcgtttc
    19201 atcatgatcg acccgaaaat gctggagctt tcggtttatg aaggcattcc gcatctgtta
    19261 acagaagtcg ttactgatat gaaagatgcc gccaacgcgc tgcgctggtg tgttaacgag
    19321 atggagcgtc ggtataaact gatgtctgcg ctgggtgtgc gtaatctggc gggttataac
    19381 gaaaaaattg ctgaagccga tcgcatgatg cgtccgattc cagacccgta ctggaagccg
    19441 ggtgacagta tggatgccca gcatccggtg ctgaaaaaag aaccatacat tgtggtgttg
    19501 gttgacgaat ttgccgacct gatgatgacg gtaggtaaaa aagtggaaga gctgatagca
    19561 cgtctggcgc aaaaagcccg tgccgcgggt atccacctcg tactggcaac tcagcgtcca
    19621 tcggttgatg ttattactgg tctgattaaa gcgaatattc cgacccgtat cgcctttacc
    19681 gtatccagta agattgactc acgtaccatt cttgatcagg ctggcgcgga atcactgctg
    19741 gggatggggg atatgctcta ctctgggccg aactccacgt tgccggtacg tgtccatggt
    19801 gcttttgttc gcgatcagga agttcatgcc gtggtgcagg actggaaagc gcgtggtcgc
    19861 ccacagtatg ttgatggcat cacttccgac agtgaaagcg aaggtggcgc gggcggtttc
    19921 gatggcgctg aagaactgga tccgttgttc gatcaggcgg tgcagtttgt cactgaaaaa
    19981 cgcaaagcgt caatttctgg cgtacagcgt cagttccgca ttggttataa ccgtgcagcg
    20041 cgtattatcg aacagatgga agcgcagggg attgtcagcg aacaggggca caacggtaat
    20101 cgtgaagtgc tggccccacc gccgtttgac taattaatgc attgccggat aaggcgcggt
    20161 agcgtcgcat ccggcactct atcaactgaa aattcagtat tttcttcttt cctcaagctg
    20221 attattagcc tggaatagag agtagaggga actcccgatc gggagtgacg taatttgagg
    20281 aataatgatg aaaaaaattg ccatcacctg tgcattactc tcaagcttag tagcaagcag
    20341 cgtttgggct gatgccgcaa gcgatctgaa aagccgcctg gataaagtca gcagcttcca
    20401 cgccagcttc acacaaaaag tgactgacgg tagcggcgcg gcggtgcagg aaggtcaggg
    20461 cgatctgtgg gtgaaacgtc caaacttatt caactggcat atgacacaac ctgatgaaag
    20521 cattctggtt tctgacggta aaacactgtg gttctataac ccgttcgttg agcaagctac
    20581 ggcaacctgg ctgaaagatg ccaccggtaa tacgccgttt atgctgattg cccgcaacca
    20641 gtccagcgac tggcagcagt acaatatcaa acagaatggc gatgactttg tcctgacgcc
    20701 gaaagccagc aatggcaatc tgaagcagtt caccattaac gtgggacgtg atggcacaat
    20761 ccatcagttt agcgcggtgg agcaggacga tcagcgcagc agttatcaac tgaaatccca
    20821 gcaaaatggg gctgtggatg cagcgaaatt taccttcacc ccgccgcaag gcgtcacggt
    20881 agatgatcaa cgtaagtaga ggcacctgag tgagcaatct gtcgctcgat ttttcggata
    20941 atacttttca acctctggcc gcgcgtatgc ggccagaaaa tttagcacag tatatcggcc
    21001 agcaacattt gctggctgcg gggaagccgt tgccgcgcgc tatcgaagcc gggcatttac
    21061 attctatgat cctctggggg ccaccgggta ccggcaaaac aaccctcgct gaagtgattg
    21121 cccgctatgc gaacgctgat gtggaacgta tttctgccgt cacctctggc gtgaaagaga
    21181 ttcgcgaggc gatcgagcgc gcccggcaaa accgcaatgc aggtcgccgc actattcttt
    21241 ttgttgacga agttcaccgt ttcaacaaaa gccagcagga tgcatttctg ccacatattg
    21301 aagacggcac catcactttt attggcgcaa ccactgaaaa cccgtcgttt gagcttaatt
    21361 cggcactgct ttcccgtgcc cgtgtctatc tgttgaaatc cctgagtaca gaggatattg
    21421 agcaagtact aactcaggcg atggaagaca aaacgcgtgg ctatggtggt caggatattg
    21481 ttctgccaga tgaaacacga cgcgccattg ctgaactggt gaatggcgac gcgcgccggg
    21541 cgttaaatac gctggaaatg atggcggata tggccgaagt caatgatagc ggtaagcggg
    21601 tcctgaagcc tgaattattg accgaaatcg ccggtgagcg aagcgcccgc tttgataaca
    21661 aaggcgatcg cttttacgat ctgatttccg cactgcataa gtcggtacgt ggtagcgcac
    21721 ccgatgcggc gctgtactgg tatgcgcgaa ttattaccgc cggtggcgat ccgttatatg
    21781 tcgcgcgtcg ctgtctggcg atagcgtctg aagatgtggg taatgctgat ccacgagcga
    21841 tgcaggtggc aattgcggcc tgggattgct ttactcgcgt tggcccggcg gaaggtgaac
    21901 gcgccattgc tcaggcgatt gtttacctgg cctgcgcgcc aaaaagcaac gctgtctaca
    21961 ctgcgtttaa agccgcgctg gcggatgctc gcgaacgtcc ggattatgac gtgccggttc
    22021 atttgcgtaa tgcgccgacg aaattaatga aggaaatggg ctacgggcag gaatatcgtt
    22081 acgctcatga tgaagcaaac gcttatgctg ccggtgaggt ttacttcccg ccggaaatag
    22141 cacaaacacg ctattatttc ccgacaaaca ggggccttga aggcaagatt ggcgaaaagc
    22201 tcgcctggct ggctgaacag gatcaaaata gccccataaa acgctaccgt taatgttatc
    22261 gttgcggtaa tgttgttact gtatccctgt ggtcgcaggc tgtggccaca tctcccattt
    22321 aattcgataa gcacaggata agcatgctcg atcccaatct gctgcgtaat gagccagacg
    22381 cagtcgctga aaaactggca cgccggggct ttaagctgga tgtagataag ctgggcgctc
    22441 ttgaagagcg tcgtaaagta ttgcaggtca aaacggaaaa cctgcaagcg gagcgtaact
    22501 cccgatcgaa atccattggc caggcgaaag cgcgcgggga agatatcgag cctttacgtc
    22561 tggaagtgaa caaactgggc gaagagctgg atgcagcaaa agccgagctg gatgctttac
    22621 aggctgaaat tcgcgatatc gcgctaacca tccctaactt gcctgcagat gaagtgccgg
    22681 taggtaaaga cgaaaatgac aacgttgaag tcagccgctg gggcaccccg cgtgagtttg
    22741 actttgaagt tcgtgaccac gtgacgctgg gtgaaatgca ctctggcctc gactttgcag
    22801 ccgcagttaa gctgacaggt tcgcgctttg tggtgatgaa agggcagatt gctcgcatgc
    22861 accgcgcact gtcgcagttt atgctggatc tgcataccga acagcatggc tacagtgaga
    22921 actatgttcc gtacctggtt aaccaggaca cgctgtacgg tacgggtcag ctgccgaaat
    22981 ttgctggcga tctgttccat actcgtccgc tggaagaaga agcagacacc agtaactatg
    23041 cgctgatccc aacggcagaa gttccgctga ccaacctggt acgcggtgaa atcattgatg
    23101 aagatgatct gccaattaag atgaccgccc acaccccatg cttccgttct gaagccggtt
    23161 catatggtcg tgacacccgt ggtctgatcc gtatgcacca gttcgacaaa gttgaaatgg
    23221 tgcagatcgt gcgcccggaa gactcaatgg cggcgctgga agagatgact ggtcatgcag
    23281 aaaaagtcct gcaactgctg ggcctaccgt accgtaaaat catcctttgt accggcgaca
    23341 tgggctttgg cgcttgcaaa acttacgacc tggaagtatg gatcccggca cagaacacct
    23401 accgcgagat ctcttcctgc tccaacgttt gggatttcca ggcgcgtcgt atgcaggctc
    23461 gttgccgcag caagtcggac aagaaaaccc gtctggttca taccctgaac ggttctggtc
    23521 tggctgttgg tcgtacgctg gttgcagtaa tggaaaacta tcagcaggct gatggtcgta
    23581 ttgaagtacc agaagttctg cgtccgtata tgaacggact ggaatatatt ggctaatacc
    23641 caatttttct gaatctaaaa agcgcctgcg ggcgcttttt ttgtctccct ttgataccga
    23701 acaataatta ctcctcactt acacgtaata ctactttcga gtgaaaatct acctatctct
    23761 ttgattttca aattattcga tgtatacaag cctatatagc gagctgctat agaaataatt
    23821 acacaatacg gtttgttact ggaatcaatc gtgagcaagc ttgagtgagc cattatgaaa
    23881 acgaaaatcc ctgatgcggt attggctgct gaggtgagtc gccgtggttt ggtaaaaacg
    23941 acagcgatcg gcggcctggc aatggccagc agcgcattaa cattaccttt tagtcggatt
    24001 gcgcacgctg tcgatagcgc cattccaaca aaatcagacg aaaaggttat ctggagcgcc
    24061 tgtacagtta actgtggtag tcgctgcccg ctacgtatgc acgtcgtgga cggtgaaatc
    24121 aaatatgtcg aaacggacaa taccggcgat gacaattacg acggcctgca ccaggttcgc
    24181 gcctgtctgc gtggacgttc catgcgtcgc cgtgtctata atccggaccg cctgaaatat
    24241 ccgatgaaac gagtcggggc gcgcggtgaa ggcaaatttg agcgcattag ctgggaagaa
    24301 gcctacgaca tcatcgcgac caatatgcag cgcctgatca aagagtacgg caacgaatcc
    24361 atctatctga actatggcac cggtacgctg ggcggcacca tgacccgctc ctggcccccg
    24421 ggaaataccc tggtcgcgcg cctgatgaac tgctgcggcg gttatctgaa ccattacggt
    24481 gactactctt ccgcgcaaat tgcggaaggt ttgaactata cctacggcgg ctgggcagat
    24541 ggtaacagcc cgtcggatat cgaaaacagt aaactggtag tgctgtttgg taataaccct
    24601 ggcgaaacgc gaatgagtgg cggtggggtg acttactatc ttgaacaggc acgccagaaa
    24661 tctaatgccc gcatgatcat catcgatccg cgctataccg acaccggtgc cgggcgcgaa
    24721 gatgagtgga tccctattcg tccgggaaca gatgccgcac tggttaacgg tctggcgtac
    24781 gtcatgatca ctgaaaacct ggtggatcag gcattcctcg ataaatattg cgttggctac
    24841 gatgagaaaa ccctgccagc cagtgcgccg aaaaatggcc actataaagc ttatattctg
    24901 ggtgaagggc cagatggcgt ggctaaaaca ccggaatggg cctcacaaat caccggtgtt
    24961 ccggcagaca aaatcatcaa actggctcgt gaaatcggca gtaccaaacc ggcgtttatc
    25021 agccagggat ggggcccgca gcgtcacgct aacggtgaaa tcgcaacccg tgctatctcg
    25081 atgctggcga ttctgaccgg taacgttggt attaacggag gcaacagcgg cgcgcgtgaa
    25141 ggttcataca gcttaccgtt tgtccgtatg ccgaccttgg aaaacccgat ccagaccagc
    25201 atttcgatgt ttatgtggac cgatgccatt gaacgtggcc cggaaatgac ggcgctgcgt
    25261 gatggtgtac gcgggaaaga taagctggat gtgccgatca aaatgatctg gaactatgcc
    25321 ggtaactgcc tgattaacca gcattctgaa atcaaccgta cccatgaaat ccttcaggat
    25381 gataagaagt gcgagctgat tgtggttatc gactgccaca tgacctcatc ggcgaaatat
    25441 gctgacatcc tgctgcctga ctgcaccgct tccgaacaga tggactttgc gctggatgca
    25501 tcctgcggga atatgtctta cgtgattttc aacgatcagg tgattaaacc gcgctttgaa
    25561 tgtaagacca tctatgaaat gaccagcgaa ctggcaaaac gtcttggcgt tgagcaacag
    25621 tttactgaag gccgtacaca ggaagagtgg atgcggcatc tgtatgccca gtcgcgggaa
    25681 gcgattcctg aactgccaac gtttgaagag ttccgcaagc aggggatctt taaaaagcgc
    25741 gacccacaag ggcatcacgt tgcttataaa gccttccgtg aagatccgca ggcaaaccca
    25801 ctgactacgc catcgggcaa aattgagatt tattcgcagg cgctggctga cattgccgct
    25861 acctgggaat tgccagaagg cgatgtgatc gatccactgc cgatctacac gccgggcttt
    25921 gaaagttatc aggatccgct gaacaaacag tatccgctgc agcttacggg tttccactat
    25981 aaatctcgcg ttcactcaac ttacggcaac gttgatgtgc tgaaagcggc ttgccgtcag
    26041 gaaatgtgga tcaacccgct tgatgcgcaa aaacgcggta tcaacaacgg cgataaagtc
    26101 aggatcttta acgatcgtgg tgaagttcat attgaggcga aagtgacgcc acgaatgatg
    26161 ccgggtgtgg tcgcactggg tgaaggtgcc tggtatgacc cggatgcaaa acgtgtcgat
    26221 aagggcggtt gtattaacgt actgaccact caacgtccgt ctcctctcgc taaggggaat
    26281 ccgtcacata caaaccttgt tcaggttgaa aaggtgtaag gagtaaccga tgacaaccca
    26341 gtatggattt tttattgatt ccagccgttg caccggttgc aaaacctgcg agctggcctg
    26401 taaagactac aaagatttga cgccagaagt cagcttccgc cgcatttatg aatatgctgg
    26461 cggcgactgg caggaagata acggtgtctg gcaccagaac gtgtttgcct actatctgtc
    26521 gatttcatgt aaccactgcg aagatccggc ttgtactaaa gtctgcccga gcggtgcgat
    26581 gcataaacgc gaagatggtt ttgttgtggt cgatgaagat gtatgcattg gctgccgcta
    26641 ctgtcatatg gcttgcccgt atggcgcacc gcaatataac gaaacgaaag gccatatgac
    26701 caaatgcgat ggttgttatg accgtgttgc ggagggtaaa aagccgatct gtgttgaatc
    26761 ttgcccgctg cgggcgctgg atttcggtcc tatcgacgag ctgcgtaaaa aacatggcga
    26821 tctggcggcc gttgcgccgt tgccgcgagc tcactttacc aaaccgaata ttgtgatcaa
    26881 acccaatgcc aacagccgcc cgaccgggga taccactggc tacctggcaa acccgaagga
    26941 ggtgtgagat gggaagtgga tggcatgaat ggccgctgat gatcttcacg gtcttcgggc
    27001 aatgtgtagc gggtggtttt atcgttctgg ctttggcgct gctcaaaggc gacctgcgag
    27061 cagaagccca gcagcgtgtt atcgcctgca tgtttggctt atgggtgctg atgggcattg
    27121 gctttatcgc ctctatgctc catcttggtt caccgatgcg cgcctttaac tcgctcaacc
    27181 gggtaggggc ttcagcactc agtaacgaaa tcgccagcgg ttcgatcttt tttgctgtag
    27241 gcggcatcgg ctggttgctg gcaatgctga aaaagctgtc accggcattg cgtacgctat
    27301 ggctggtggt gacgatggtt cttggcgtcg tctttgtctg gatgatggtg cgtgtgtata
    27361 acagcattga taccgttccg acctggtaca gcatctggac gccgatgggc ttcttcctga
    27421 cgatgttcat gggcggcccg ttactgggtt acctgttgct gagtctggct ggcgtcgatg
    27481 gctgggcgat gcgtctgctg ccagccattt ctgtactggc actggtggtg agtggtgtga
    27541 tgtcagtgat gcagggcaca gagctggcaa ctattcatag ttctgtgcag caggccgcag
    27601 cgctggtgcc ggactatggt gcgctgatgt cctggcggat cgtgcttttg gccgttgccc
    27661 tgtgcttgtg gattgcacca cagctaaaag gttatcagcc tgcggttccg ctactatccg
    27721 tctcattcat tctgcttctg gcaggggaat taatcggtcg cggcgtattc tatggtttgc
    27781 atatgaccgt ggggatggcc gtcgcaagct aatcagtaac aaccggggtt tcggccccgg
    27841 ttctctttta tttctgcttc gttaacgtgt cataactggt catcaaatta cgataatcag
    27901 gaatatggtt agagaacaac gtcgccagtc cttcaatatc attacgccag tcacggtgca
    27961 gctcgcacgc cacgccgaac catgtcatca attgcgcacc tgcttgcgac atgcgatccc
    28021 atgccgaatg ccgggtaatt tcattaaagg tccctgacgc gtcagtcacg acaaagacgt
    28081 caaacccttc ttcaatggct gaaagcgccg ggaatgccac gcaaacttcg gttaccacac
    28141 cggcaataat taactgtttt ttacctgtcg ctttgactgc ttttacaaaa tcttcgttat
    28201 cccaggcgtt aatatttccc gggcgagcaa tgtaaggtgc atcggggaat tgtgctttca
    28261 gttctggaac taatggaccg ttaggaccgg tttcaaaact ggtggtaaga atagttggta
    28321 agttgaaata cttagccagg tcacccagcg ccagcacgtt atttttaaac ttatcgggtt
    28381 cgatatcccg tacaagggaa agtaaaccag cctggtgatc aacaagcaaa acggcagcat
    28441 catttttatc aagacgaaca tacggtttgg tcatcctctt ctcctttcga gagtcagtgg
    28501 tacgtctgaa atcggtcaga cgcgatgcat tgctctgaaa gcatagacgg gaaatatgag
    28561 tttgctgtga ccatgaaatt tttcgactga acgaagcgta tgaaatttgt gttagatcaa
    28621 taaaaacaat cagatgagat taatcgtctc ttcagcatta gcgttatgaa tgagtaatat
    28681 aaatcgataa taccgccgct gcgatatgcg cgtattttca cccattgaca atgtttttgg
    28741 cggtggcatg atgcgcatga aatttgaact tcctcacggt tttaattcat gtccacgtat
    28801 acccggcctg tcatgctttt gctgtctggc ctgcttttgt tgactctggc gatcgcggtg
    28861 ttaaatacac tcgtgccgct ttggctcgcc caggaacaca tgtccacatg gcaggtaggc
    28921 gttgtcagct catcctattt taccggcaac cttgtcggta cattgctgac agggtatgtc
    28981 attaagcgca ttggctttaa ccgcagctat tatctggcct ccttcatttt tgccgctggc
    29041 tgtgccggcc ttggcctgat gattggattc tggagctggt tggcttggcg ttttgtcgcg
    29101 ggcgtcggct gtgccatgat ttgggtggtt gttgagagcg cgctgatgtg cagtgggacg
    29161 tcacgtaacc gtgggcgttt gcttgctgcg tatatgatgg tttattacgt gggaacgttt
    29221 ttaggccagt tactggtcag caaagtttca accgagctga tgtccgtatt gccgtgggtt
    29281 acaggtttga cgttggcagg gatcttaccg ctgttgttta cgcgtgtgct gaatcagcag
    29341 gctgaaaacc atgattcgac gtcaattacg tcaatgctaa aactccgtca ggcgcggctt
    29401 ggcgtgaatg gctgcattat ctcaggaatc gttctgggat ctctatatgg cctgatgccg
    29461 ctgtacctca atcacaaagg ggtgagcaat gccagcattg gtttctggat ggcggtactg
    29521 gtcagtgcgg gtatccttgg acaatggccg attggacgtc tggcggataa gtttggtcga
    29581 ctgctggtgt tgcgtgttca ggtctttgtc gtcattctcg gcagtatcgc gatgcttagc
    29641 caggcggcga tggccccagc gttattcatc ctcggtgccg ctggctttac gctatatccg
    29701 gtggcgatgg catgggcttg cgagaaagtt gaacatcatc aactggtggc gatgaaccag
    29761 gccttactgt tgagttatac tgtgggaagt ctgcttggcc cgtcatttac cgctatgcta
    29821 atgcagaatt tctccgataa tttattgttt atcatgatcg ccagcgtatc gtttatctat
    29881 ttgctgatgc tgctgcgcaa cgccggtcat acgccgaaac ccgttgctca cgtgtaaatg
    29941 aattcaagca gagtgtgaac ttactgtttc acactctgct tttttgtttc ttctatctga
    30001 cttgctttat tccaaatttt attcgtttaa aaataaaatg tgcagcaggt tataattttg
    30061 catttcgcta tttccgcact tcttatttgc cgcgcataat ccctcgtttt acagatgccc
    30121 ctttaatttt ggcgaaggat ttgtctatgg ctgggaatgt tcaggaaaaa cagttgcgat
    30181 ggtacaacat tgcgctgatg tcttttatca ctgtctgggg ttttggcaac gttgttaata
    30241 actatgccaa ccaggggctg gtggttgttt tttcatgggt gtttatcttt gcactctatt
    30301 tcacacctta tgcgctaatt gttggtcagt taggctcgac cttcaaagat gggaagggcg
    30361 gggtcagtac ctggattaaa cacacgatgg gacccggact ggcttatctc gccgcgtgga
    30421 cctactgggt ggtgcatatt ccctatctgg cacaaaaacc ccaggcaatt ctgattgcgc
    30481 tcggttgggc gatgaaaggc gacggttcgc taatcaaaga atattcagtc gtagcgttac
    30541 aggggttaac gctggtgctg tttatcttct ttatgtgggt tgcttcacgc ggtatgaaat
    30601 cgctgaaaat cgtcggttct gtggcaggga ttgcgatgtt tgttatgtcg ctcctgtatg
    30661 tggcgatggc ggtaaccgcg cctgcgatta ctgaagtgca tattgcgacc acaaacatta
    30721 cctgggaaac gttcattcct catatcgact ttacctacat taccactatt tcaatgctgg
    30781 ttttcgcggt tggcggagca gagaagattt ctccttacgt taatcaaacg cgcaacccag
    30841 gaaaagaatt tccaaaaggg atgctatgcc tggcggtgat ggttgcggtt tgtgccattc
    30901 tgggctcact ggcgatgggg atgatgtttg actcgcgtaa tatcccggat gacttaatga
    30961 ctaacggtca atattacgcc tttcagaagc tgggcgagta ttacaacatg ggtaatactt
    31021 taatggtgat ttacgccatt gcgaataccc tgggacaagt agctgcgctg gtattctcga
    31081 ttgatgcccc gcttaaagtg ctattaggtg atgctgacag caaatatatt ccagccagtt
    31141 tatgtcgtac caacgcttct ggtacgcccg ttaatggcta ttttctgacc ctggtgctgg
    31201 tggcaattct gattatgctg ccgactctcg gcatcggtga tatgaacaat ctctataaat
    31261 ggctgttgaa tcttaactcg gtagtgatgc cgctgcgtta tttatgggta tttgttgcat
    31321 ttattgcagt cgttcgcctg gcgcagaaat ataaaccaga atatgtcttt attcgtaata
    31381 agcctctggc aatgaccgtc gggatttggt gttttgcctt taccgccttt gcctgcttga
    31441 cggggatctt cccgaaaatg gaagccttca ctgcagagtg gaccttccag ttggcgctga
    31501 atgttgcaac gccgtttgtg ctggtaggat taggactgat attcccgctg ctggcgcgta
    31561 aagcgaatag taaataatta ttgtggtcgg tgacgctgac tctggcagat ctgacccgtt
    31621 tttacataat cgagaaatgc gcgcaagccg caggacatat actggcggtt tggataatag
    31681 atctggaagc ctgaacgctc tgtgctccag tcttccagta cacattctaa acgaccagta
    31741 tcaaggtact ccttgatctg ttcatagagc agatacccga taccagcacc catcaaaacg
    31801 gcctccagtt cagcatcaac atcatccaga atgatatttc ctgcgacagc aatttccagc
    31861 tcctttgcaa attgccagtg gaaaggtttg ccgctgggat aacgaaaaac aacacattga
    31921 tgattaagca gatcatgagg atggcggggt ttgccatatc ttgcaaagta ctctggcgtt
    31981 gctgctacac ataatttcac tggtgggccg atcgccacgc tgatcatatc tttttcgacg
    32041 atgccactaa gtcgaacgcc agcgtcaaag ccctgttgga cgatatcgac aagagaatcg
    32101 tcagttgtga gctcgacttt aatatcagga tattcacgtg tgaaaccaac cagtaaaggc
    32161 attaaaaata tacgtgcagc tacccgggcg gcattgattt tgagcgttcc tgtcggtgtc
    32221 agacgaaaat cattcatctc atctaacatg atctgaattt cgtcgaaagc tgggcgcaat
    32281 cgttcataga gattagaacc cgcttccgtt aacgatacgc ttcgggttgt ccgattgaat
    32341 aagcgaattt taagacgttg ttccagtgtt ttaatactat ggctaatggc ggacgaggat
    32401 aagcctaatt catcgcccgc tgcacgaaag ctttgattac gggccacggc aaagaaagtg
    32461 gcaaagtcag acatattcat ccgcattagt gaaaatcctt cattaactca tcctgaatca
    32521 tcacgtttac aggattatgc cgcctgcgta aagtgcgctc cagaacttaa cgtggaggta
    32581 aaattatgca gtctgaacgt atttatttgg tatgggccca tcctcgtcat gattcattga
    32641 ccgcacatat tgctgatgcg atccatcagc gggcaatgga gcggaaaata caggtgacgg
    32701 aactcgattt atatcggcgt aatttcaacc cggtgatgac gtcggaagat gaaccagact
    32761 ggaagaatat ggataaacgt tattctccag aggttcatca gctttattca gagctgcttg
    32821 aacatgacac gttagtggtg gtttttcctc tctggtggta cagcttccca gcaatgctaa
    32881 aaggatatat tgacagagta tggaataatg ggctggctta tggagatggg cacaaattac
    32941 cattcaataa agttcgttgg gtggcgctgg ttggaggaga caaagaatca tttgtccaga
    33001 tgggctggga aaaaaatata agcgattatt tgaaaaatat gtgcagttat cttggtattg
    33061 aagatgccga tgtcactttc ttgtgtaata cagtggtatt cgatgggcaa gaacttcatg
    33121 cgagctatta tcagtcgtta ttatctcagg tacgggatat ggtagatgca ctataaaatg
    33181 tgttaaaaac gctgtagcag aatgaagcgc ggaataaaaa agcggcaact caataaagtt
    33241 gccgctttac ggggaaatta gaacattacc ttatgaccgt actgctcaag aatgcctttc
    33301 acgcgttcca tggtctcttt cttaggtggt ttaacaccgt cgagtttgta ctcttcaccc
    33361 attgccaccc atttgtgttt gcccagctcg tggtagggga ggagttcgat tttctcaacg
    33421 ttgcccatat cacgggtaaa ttcaccgagg cgatgcgctg aatcgtcatc gtcagaccag
    33481 cctgggacaa caacgtagcg gatccacacc ttcacatttt tgttcgccag atatttggcg
    33541 aactccagcg tgcggtggtt ggaaactcca accagatttt ggtggatctc gtcgttcatc
    33601 tgtttgagat cgagcattac caggtcggtt acttccagca gttcatcaat caccggatcg
    33661 taacgacgaa caaaaccgtt ggtgtccaga caggtatgaa tgccttcttt tttgcaggcg
    33721 cggaaccagt cacgaacaaa ctcagcttgt aggattgcct caccgccgga tgcggtaacg
    33781 ccgccgccgg aagcgttcat aaagtggcga taggtcacca cctctttcat taaatcttca
    33841 acggtaactt ctttaccgcc atgcgtatcc caggtgtcgc ggttatgaca atacaggcag
    33901 cgcatcaggc agccctggaa aaaggtgata aagcgaatac ctgggccgtc tacggttcca
    33961 caggattcaa aggagtgaat gcgaccaata actgacattg cggtgtttct ccagatgtgg
    34021 cccatctgag gccgtgttgg tgcgcagctc gaaggctacg tcgagtctgt tttggcagtc
    34081 accttaaagt atagatagct gacaaaaaag gctctcgtgc taaaaaaggc cccactttcg
    34141 tggagccttt attgtacgct ttttactgta cgatttcagt caaatctaat tacatagatt
    34201 gagtgaaggt acgagtaata acgtcctgct gctgttcttt agtcagcgag ttgaaacgta
    34261 ctgcgtagcc agatacacgg atggtcagct gcggatattt ttccgggttt tccatcgcgt
    34321 cgagcagcat ttcacggttc atcacgttaa cgttcaggtg ctgaccacct tcgatggatg
    34381 cttcgtggtg gaagtaacca tccatcagac cagccaggtt ggtcttacga acttcgtcgt
    34441 ctttacccag tgcgttcgga acgatagaga aggtgtagga gataccatct ttagcgtaag
    34501 caaacggcag tttagcaacg gaagtcagag acgctacagc acctttctgg tcacgaccgt
    34561 gcatcgggtt agcacccggt ccgaacggcg cgccagcacg acgaccgtct ggggtgttac
    34621 cagttttctt accatacaca acgttagaag tgatggtcag aacagactga gtcgggatag
    34681 cgtcacggta ggtgtgcagt ttctgaattt tcttcatgaa acgttctacc aggtcaacag
    34741 ccaggtcatc tacacgcgga tcattgttac caaactgcgg gtattcgcct tcgatttcga
    34801 agtcgatagc cagaccgtct tcgtcacgaa tcggtttaac tttcgcatat ttgattgcag
    34861 acagggagtc agcagcaacg gacaggccag cgataccaca cgccatggtg cggataacgt
    34921 cacggtcgtg cagcgccatc agagaggctt cgtagctgta cttgtcgtgc atgtagtgga
    34981 tgatgttcag tgcagtgatg tactgtttag ccagccagtc catgaagtga tccatgcgct
    35041 ccatcacttc atcatagttc aggacatcgc ctttgatcgg ttcagacttc ggaccaacct
    35101 gcattttcag tttttcgtca acgccgccgt tgattgcgta cagcatggtt ttcgccaggt
    35161 ttgcacgcgc accgaagaac tgcatttgtt taccaacgat catcgggctt acgcagcaag
    35221 caatagcgta gtcatcgttg ttgaagtccg gacgcatcag gtcatcgttc tcatactgca
    35281 gagaagaggt gtcgatggac actttagcgg cgaatttctt gaagttcagc ggcagttttt
    35341 cagaccacag aatggtcatg ttcggttccg gagacggacc catggtgtac agggtgttca
    35401 ggaaacggaa gctgtttttg gtaaccaggg tacgaccgtc gaggcccata ccaccgatag
    35461 attcggttgc ccagatcggg tcgccagaga acagttcatc gtattccgga gtacgcagga
    35521 agcgaaccat acgcagtttc atgaccaggt ggtcaaccat ttcctgcgct tcttgttcgg
    35581 tgatcttgcc agctttcagg tcacgttcga tgtacacatc caggaaggtg gaggtacgac
    35641 cgaaggacat tgcagcaccg ttctgagact taacagcagc caggtagccg aagtaagtcc
    35701 actggatagc ttcctgagcg ttggtagccg gaccagagat gtcgtagccg tatttcgcag
    35761 ccatttcttt catctgaccc agagcgcggt gctgttcagc gatttcttcg cgcagacgga
    35821 tagtctgttc caggtttacg ccgttttcca gatcagcctg cagagaagtg aactgtgcca
    35881 gtttgtcttt catcaggtag tcgataccgt acagcgcaac gcgacggtag tcaccgatga
    35941 tacggccacg gccatatgca tctggcagac cggtcagaac accagattta cggcaacgca
    36001 ggatgtccgg agtgtaaacg tcgaacacgc cctggttgtg agttttacgg tattcagtga
    36061 agattttttt gatcatcgga tccagttcgc ggttgtacgc tttgcaggaa ccttcgatca
    36121 ttttgatacc accgaacggg ataagagcac gtttcagcgg agcttcagtc tgcagaccaa
    36181 cgattttctc aagctgcttg ttgatgtagc cagcgtcgtg agaggtgatg gtggaagcaa
    36241 cagcggtgtc aaagtcaact ggcgcgtgag tgcggttttc cagtttaacg ccttccatta
    36301 ctttgtccca cagggtggtg gtcgcttcag tagcgccagc caggaaggac tcgtcaccct
    36361 cgtacggagt gtagtttttc tgaatgaagt cacggacgtt tacttcattc tgccagtcac
    36421 ctttggtaaa accttcccag gctgtggcta acttttcatt aagctcggac atgtaacacc
    36481 taccttctta agtggatttt ttatttactg cgtacttcga caaccattaa tggtggtcgt
    36541 tttcacgcag gtaaatgacc cagtatgtca acccaaccaa caaaccacca ccgataatgt
    36601 tgccgatcgt aaccggaatc aggttatcag tgatgaaatt catcacggtc aggtgagaaa
    36661 aattttccgg tgcagaaccg actgcggtcc aaaattccgg ggatgcgaag tcgcggatta
    36721 caatacccat cgggatcata aacatgtttg cgatactgtg ctcaaaaccg ctggcaacaa
    36781 acatcgcgac cggcagcacc ataatgaacg ctttgtccat caggctgcgg ccagaataac
    36841 tcatccatac tgccagacat accatcaggt ttgccaggat accaagacag acggcctcaa
    36901 taaaagtatg gtgcactttg tggtcggcgg tttgtaggac gtttagtccc cattgaccat
    36961 ttgcggtcat atactcgccg gaaagccaca ttaaaagtac aaacagcagt gcgccgacca
    37021 ggttgccaaa atagacattt agccagtttt tcgccaactg accccaggtg atgcgcccac
    37081 tcgccttagc aacaacaatc aacacggtgg aagtaaagag atcggctccg cagacaacac
    37141 aaagaatcag ccccagagag aagcaaatgc cgccaaccag ttttgccatg ccgaagggca
    37201 ttgtgcctgt gccagtggtt gctgtgatat agaagacgaa tgcgattgag atgaaaacac
    37261 cggcggtaat cgccagatag aaagtcttaa gcggatgttt cgttgcttta tagacacccg
    37321 cctcttcggc cactttggcc attgcagcag gaagtaaaag atcaaaaggg ttgtcagctt
    37381 tcacactaac tctctcttta ttaagtcggc gacgagatac taacaaagca ttatagatga
    37441 gaaattgata tagatcatat ctcgcctggc ttataggccc gtaactcgca tggtttttat
    37501 gcaaatacgg agtaaatatt tgattatcca aataaaaata aattttaaaa attaacaaat
    37561 gagttgaatt ttttccgcat cctccgctaa aacagttaat taaaagggag catcaggcg
//
LOCUS       DAZFXT010000036        37338 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.036, whole genome shotgun sequence.
ACCESSION   DAZFXT010000036 DAZFXT010000000
VERSION     DAZFXT010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37338
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.036"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(117..359)
                     /gene="yiiF"
                     /locus_tag="ACHKW0_001916"
     CDS             complement(117..359)
                     /gene="yiiF"
                     /locus_tag="ACHKW0_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YiiF"
                     /protein_id="HFO7474760.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(578..796)
                     /gene="yiiE"
                     /locus_tag="ACHKW0_001917"
     CDS             complement(578..796)
                     /gene="yiiE"
                     /locus_tag="ACHKW0_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HFO7474761.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(1382..1672)
                     /gene="nadS"
                     /locus_tag="ACHKW0_001918"
     CDS             complement(1382..1672)
                     /gene="nadS"
                     /locus_tag="ACHKW0_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NadS family protein"
                     /protein_id="HFO7474762.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(1673..1984)
                     /locus_tag="ACHKW0_001919"
     CDS             complement(1673..1984)
                     /locus_tag="ACHKW0_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7474763.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            2213..3121
                     /locus_tag="ACHKW0_001920"
     CDS             2213..3121
                     /locus_tag="ACHKW0_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7474764.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(3185..4126)
                     /gene="fabY"
                     /locus_tag="ACHKW0_001921"
                     /gene_synonym="yiiD"
     CDS             complement(3185..4126)
                     /gene="fabY"
                     /locus_tag="ACHKW0_001921"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HFO7474765.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAIADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(4171..4608)
                     /gene="dtd"
                     /locus_tag="ACHKW0_001922"
     CDS             complement(4171..4608)
                     /gene="dtd"
                     /locus_tag="ACHKW0_001922"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HFO7474766.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(4605..5477)
                     /gene="yihY"
                     /locus_tag="ACHKW0_001923"
     CDS             complement(4605..5477)
                     /gene="yihY"
                     /locus_tag="ACHKW0_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HFO7474767.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(5471..6070)
                     /gene="yihX"
                     /locus_tag="ACHKW0_001924"
     CDS             complement(5471..6070)
                     /gene="yihX"
                     /locus_tag="ACHKW0_001924"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HFO7474768.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(6169..6954)
                     /gene="csqR"
                     /locus_tag="ACHKW0_001925"
     CDS             complement(6169..6954)
                     /gene="csqR"
                     /locus_tag="ACHKW0_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418320.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HFO7474769.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEEKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(6988..7884)
                     /gene="yihV"
                     /locus_tag="ACHKW0_001926"
     CDS             complement(6988..7884)
                     /gene="yihV"
                     /locus_tag="ACHKW0_001926"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HFO7474770.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDTGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            8052..8948
                     /gene="yihU"
                     /locus_tag="ACHKW0_001927"
     CDS             8052..8948
                     /gene="yihU"
                     /locus_tag="ACHKW0_001927"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HFO7474771.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     SGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            8972..9850
                     /gene="yihT"
                     /locus_tag="ACHKW0_001928"
     CDS             8972..9850
                     /gene="yihT"
                     /locus_tag="ACHKW0_001928"
                     /EC_number="4.1.2.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HFO7474772.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSGVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            9867..11108
                     /gene="yihS"
                     /locus_tag="ACHKW0_001929"
     CDS             9867..11108
                     /gene="yihS"
                     /locus_tag="ACHKW0_001929"
                     /EC_number="5.3.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HFO7474773.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYSLVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTAYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVTTKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            11246..12148
                     /gene="yihR"
                     /locus_tag="ACHKW0_001930"
     CDS             11246..12148
                     /gene="yihR"
                     /locus_tag="ACHKW0_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HFO7474774.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTATSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HTYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKTIAATKIDH
                     TFKTANDLWEITITHPQQALSVSLCCDQPWVQIYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            12347..14383
                     /gene="yihQ"
                     /locus_tag="ACHKW0_001931"
     CDS             12347..14383
                     /gene="yihQ"
                     /locus_tag="ACHKW0_001931"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HFO7474775.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHSKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRMTSFGKRVMWNWKWNSENYPQLDSRIKQWNQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGISAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTSVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYTLKYQYLLGRDILIAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLKSI"
     gene            14429..15814
                     /gene="yihP"
                     /locus_tag="ACHKW0_001932"
     CDS             14429..15814
                     /gene="yihP"
                     /locus_tag="ACHKW0_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474776.1"
                     /translation="MSHITTEDPATLRLPFKEKLSYGIGDLASNILLDIGTLYLLKFY
                     TDVLGLPGTYGGIIFLISKFFTAFTDMGTGIMLDSRRKIGPKGKFRPFILYASFPVTL
                     LAIANFVGTPFDVTGKTVMATILFMLYGLFFSMMNCSYGAMVPAITKNPNERASLAAW
                     RQGGATLGLLLCTVGFVPVMNLIEGNQQLGYIFAATLFSLFGLLFMWICYSGVKERYV
                     ETQPTNPAQKPGLLQSFRAIAGNRPLFILCIANLCTLGAFNVKLAIQVYYTQYVLNDP
                     ILLSYMGFFSMGCIFIGVFLMPGAVRRFGKKKVYIGGLLIWVLGDLLNYFFGGGSVSF
                     VAFSCLAFFGSAFVNSLNWALVSDTVEYGEWRTGVRSEGTVYTGFTFFRKVSQALAGF
                     FPGWMLTQIGYVPNVAQADHTIEGLRQLIFIYPSALAVVTIVAMGCFYSLNEKMYVRI
                     VEEIEARKRTA"
     gene            15857..17260
                     /gene="yihO"
                     /locus_tag="ACHKW0_001933"
     CDS             15857..17260
                     /gene="yihO"
                     /locus_tag="ACHKW0_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474777.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKFYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATALFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEEKTASVTL"
     gene            17328..18020
                     /gene="ompL"
                     /locus_tag="ACHKW0_001934"
     CDS             17328..18020
                     /gene="ompL"
                     /locus_tag="ACHKW0_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HFO7474778.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSIGS
                     GGAVYLDVNYKFVPWFNLTVRNRYNHNNYSSTDLSGELDNNDTYEIGTYWNFKITDKF
                     SYTFEPHYFIRVNDFNSSNGKDHHWEITNTFRYRINEHWLPYFELRWLDRNVEPYHRE
                     QNQIRIGTKYFF"
     gene            complement(18111..19376)
                     /gene="yihN"
                     /locus_tag="ACHKW0_001935"
     CDS             complement(18111..19376)
                     /gene="yihN"
                     /locus_tag="ACHKW0_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474779.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASKNTSMTSVLKD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(19478..20458)
                     /gene="yihM"
                     /locus_tag="ACHKW0_001936"
     CDS             complement(19478..20458)
                     /gene="yihM"
                     /locus_tag="ACHKW0_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HFO7474780.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(20466..21176)
                     /gene="yihL"
                     /locus_tag="ACHKW0_001937"
     CDS             complement(20466..21176)
                     /gene="yihL"
                     /locus_tag="ACHKW0_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7474781.1"
                     /translation="MTGNQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(21393..23216)
                     /gene="typA"
                     /locus_tag="ACHKW0_001938"
     CDS             complement(21393..23216)
                     /gene="typA"
                     /locus_tag="ACHKW0_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HFO7474782.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            23589..24998
                     /gene="glnA"
                     /locus_tag="ACHKW0_001939"
     CDS             23589..24998
                     /gene="glnA"
                     /locus_tag="ACHKW0_001939"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HFO7474783.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            25172..26221
                     /gene="glnL"
                     /locus_tag="ACHKW0_001940"
     CDS             25172..26221
                     /gene="glnL"
                     /locus_tag="ACHKW0_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HFO7474784.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            26233..27642
                     /gene="glnG"
                     /locus_tag="ACHKW0_001941"
     CDS             26233..27642
                     /gene="glnG"
                     /locus_tag="ACHKW0_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HFO7474785.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            27754..27864
                     /gene="yshB"
                     /locus_tag="ACHKW0_001942"
     CDS             27754..27864
                     /gene="yshB"
                     /locus_tag="ACHKW0_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HFO7474786.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(28054..29427)
                     /gene="hemN"
                     /locus_tag="ACHKW0_001943"
     CDS             complement(28054..29427)
                     /gene="hemN"
                     /locus_tag="ACHKW0_001943"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HFO7474787.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(29616..30125)
                     /gene="yihI"
                     /locus_tag="ACHKW0_001944"
     CDS             complement(29616..30125)
                     /gene="yihI"
                     /locus_tag="ACHKW0_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HFO7474788.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            complement(30244..30410)
                     /locus_tag="ACHKW0_001945"
                     /pseudo
     CDS             complement(30244..30410)
                     /locus_tag="ACHKW0_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546704.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            30707..31339
                     /gene="yihA"
                     /locus_tag="ACHKW0_001946"
     CDS             30707..31339
                     /gene="yihA"
                     /locus_tag="ACHKW0_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HFO7474789.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(31721..34507)
                     /gene="polA"
                     /locus_tag="ACHKW0_001947"
     CDS             complement(31721..34507)
                     /gene="polA"
                     /locus_tag="ACHKW0_001947"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HFO7474790.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            34871..35803
                     /gene="yihG"
                     /locus_tag="ACHKW0_001948"
     CDS             34871..35803
                     /gene="yihG"
                     /locus_tag="ACHKW0_001948"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HFO7474791.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(35844..37274)
                     /gene="yihF"
                     /locus_tag="ACHKW0_001949"
     CDS             complement(35844..37274)
                     /gene="yihF"
                     /locus_tag="ACHKW0_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HFO7474792.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYQNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLEVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
BASE COUNT         9174 a         9518 c         9001 g         9645 t
ORIGIN      
        1 aattctgcaa tatattgaat ttgcatgact ttgtaggcct gataaggcgc tcacgccgca
       61 tccggcatga acaacgcgca cgttgtcagc aatctgattg acatcatttt ttcccattac
      121 cattcctcgc gcagcttacg ctgatattcg acaccatctt cctcacaccc ttgccagatg
      181 ccaggaacac tggttcttgc tgtttgcgat tgatttatca ggtattgatc taccgcttcc
      241 cttaataggt ccgcacgagg aagattacgc tgcacctcga gatcatcaag ttgcttaatc
      301 acctcattcg ataaatcgag taaaattctg cccatatcta tacctgccag agagttcata
      361 aatatctcgc caatatcatt tttgaatcta tggagagaaa agtacccttg ccgaatcttt
      421 aaagaaagct catttacgca tcatttttta tttttgcgag gcgttttcca gatacgatgt
      481 aacagttgca gttattataa gttcaagtaa aacagccttg tattaatacc agaataaggg
      541 catctgtttt tttatattca agaatgaaaa atttttttca ttccttatgc tccttacact
      601 gatcatcaac accaactacc tccccctttt gcctgcctcc ctttgcttta cgcaccggaa
      661 attgctgccg ctccagatat tgctcaaccg cttcgcgtaa aattgcagat cgtgaaacct
      721 tacgccagcc ccgaagtttg ttcagccgtt taattgcctc ttctgataaa tgaagaaaaa
      781 ccgtattcat cgccattacc accaccttac ctatattact cattaatgta tgtgcgaaaa
      841 ttacgccgga aaatcattta aaaaaagcgg atttttgcat gttgttttgt ttttgcgagg
      901 cgttttccag aaataataag agtgttgcag ttgtttcatt tattactcaa aaatggttta
      961 gttatcgccc aggatgaatt atgggtgcta tgaatatatt atattcagag atatttattt
     1021 gcataatctg aatggtgtag ccgaaaaacg tcagcatcct aacagcacca ggatttaagg
     1081 tgaaattaat catccatcac agaccggtca gtactgccac caaccggtct ttacttgttc
     1141 atcgttataa ttttgttggt aacagttcgg gaaacaagga attccttccg gaaccccccg
     1201 gtatcatccg atagcattga cgtcaatcat cagcgcgacg atgagttttt ttaatcgcat
     1261 catagggtgg cgacgttcag cacgattacg ggtcatagca aaactcctgg gcaggcgacc
     1321 actatgccgc tttcacagga gttctgaatg ctagtgcttt ctgtatggca taaccatgcc
     1381 tctaacggtt aagtgcttgc agcacatgtt gcggatcatt ggcaatcgct cgcagcaaag
     1441 cctttgcagg cccggtcggt gaacgtcgcc cctgctccca gttacgcaac gtgtctacgc
     1501 ttacagaaat aagatcggca aatttagatt gtgacaaccc tgaagcgcgg cgtatttctt
     1561 tgattttcat agcatcaact tcaaaagtcc gcgaaggctg tcgcgttcct tcaataattt
     1621 cattcatctg ggccatgctg tgagtcaacc gctcaaaaag tgccttatcc atttaccacc
     1681 tcgtatttaa caaccggagc attgcttttt cctggggaga cagatcatct ttaataccct
     1741 tacgataaat aagcagtaat ctgatctcat aatgtttaac ctgatgaaag taaataaccc
     1801 tgacaccagc acgtttcccc tttcctcctg aaacccatcg aacctttctt aacccaccag
     1861 tttcaggtat tacctcacca tagtcaggat tcaaggccag gaaaaactga aaacgactga
     1921 attcatcgtc ggtcagcagt ttttgaacat cttcagtaaa aatttccgtt tcgatgaaga
     1981 gcataattat acgccattgg cttagagtaa caatcctttt tgtacgtcat tgacgtaccc
     2041 ttgaaaccac tagcttactg atagtgctcc agcaaaacca cgtctctgcc gggtttgtct
     2101 ttagatttgc gaggcgcttt ccagattttt cacgcccgtg aatttccctg tatccatcca
     2161 gcaaaaacgg gtgtataaga ctgtacattc atacagcaaa ataagttcta ctatggcact
     2221 ggaaaaagga attgcagagt tagtcgaaaa gtttatcgct gcgggtcgcc cctcatcgcg
     2281 tcaacaaagt attgcgcagc gcagggaggg ctatatcgcc agcgctgtcc ttgcgggtga
     2341 gacagaaacg cgggtagata ttcagactat cgaacttgaa gggatgacct tacgaatagt
     2401 gtcgccgctg aatgccccga cattactccc gacgataatt tattactacg gtggctgttt
     2461 tgttagcggc gggttcgcaa ctcacgacaa ccagctgcgc cagttagcct attacgggca
     2521 atgtcgggtt attgccgtcc agtatcggct ggcaccggaa catactttcc ccgccgccca
     2581 tgatgatgcg caacgaggtg cagaaatagt ctggcaacac gccgaacgac tcggcgttga
     2641 taaacaacgt ataactcttg ccggtgacag tgcgggcgga catctggcgc tggtaactgc
     2701 tttgcgatta aagcgtgctg gcgagtggca accggcacaa ctcatcttaa tttatcctat
     2761 gcttgatgcg actgcgcatt tcgaaagcta catacgcaat ggtcacgatt acatcatcac
     2821 acgcgacact ttactgagcg gctttgagat gtatctgcca ggcattgaac gcaggcatcc
     2881 tgaagccagc cctctttggc gcgatgattt caatggcttg ccacctgtac atatcattac
     2941 cgctgaatac gacccacttt gcgatgaagg tgaagcgttg tatcaccgca tgacagggca
     3001 aggagtgcaa tgcacctgcc aacgctacct tggcgttatc cacggatttt ttcagttagg
     3061 tggtatcagc gaggccgcgc gatcggcact acgggatgtt gcctggagag cgggaaggta
     3121 acttcggacc tgggccctga aaaggttttc agattgaaaa ccttttcagt caagtaaaac
     3181 gcccctactc ttcttcgttc ccgccctctt catacgggcc aaatggcttc gcgggcagaa
     3241 cgatatacgt gccttcaaac actgcacccg gcgtctcgtc gccaaagatt tcaacttgca
     3301 tctgcacccg tgcttttcgt ccgcgcgcca gacggtcgag atcgccgctt aaggcaccaa
     3361 ggtcggctat cgcatgaggt ttaccgctaa tcggcttgct gtagcggata tgcgcatccg
     3421 ccagaataat cgttccgccg agatggcgtt cgcgcagcat cagccagata agcccccaac
     3481 cggtgagcgt cgccagcgag aataaactcc cggcaaacag cgtatggtgc ggattctgat
     3541 tgccggtttc cggcatggtg gtgataaatt tttgcccggt atattgttga atgcgcacgc
     3601 ccattttttc gcttaatgga atgtgttcat accacgcctg ttgcagctgg gcgcaccagt
     3661 caccgcgatg cagaatatca tccagagtgg caacgggttt aatcatcaaa aaatggcgaa
     3721 tcggcgtggt agttggggtg gtgatctccc cctgattaat gaatcccagc ttggcgaaaa
     3781 actccaccgc gtcttcacgg gcgctacagg tcacgcgctt aacgccttcc tgacgcgcca
     3841 ccgactccag ggtcatcgcc atcagcgttc ctaacccttt gtcctgcacg tcgggatgaa
     3901 cggccataaa gcgaatggac gcttcattgt cggcattaat atacaatcgg cctaccgcca
     3961 ccagattacc ctgctcgtcg acgaccatct ggtgatgcgc catcgcatcc cacgcgtcgc
     4021 gttccgaacc ttttggttga tgcaggggct tacgcaacat ttcccagcga aactgatagt
     4081 aacgctctaa ttcttcttct gtttgtggaa cccgaaggtg atacatagcg atactctctc
     4141 ttgtttcccg tgacaaccct ggaagctggc tcatacctgc aaccagaatg tcacggggcc
     4201 atcattgacc agcgatacct gcatatcggc agcgaagcgt cctgtttgcg tgttcatctc
     4261 ttgctggcgg cagcgttcga cgaaatagtc atataacgcc tctgcgcgat ccggtgatgc
     4321 gcctttggag aaacttgggc gcatccctcg ttcggtatct gcggcgaggg taaactggga
     4381 aaccaccagt acactgccgc ccgcctgttg cacgttgaga ttcatcttgc cttcggcatc
     4441 gctaaagatg cggtagccga gcacacgctc gcacagacgg tttgctttct gttcgtcgtc
     4501 atccttttcg acacccaata acaccaaaag tcccgcgcca atttcgcccg tcacttctcc
     4561 ctccacggtg acgctggcac gggttacgcg ttgaattaat gcaatcatgg ttcgtcgtct
     4621 tcttcttgtt cagctgcttg ttttagtttg cggtattccc cgagagtgac agtaatttcc
     4681 gcgccaagca agacgataca ccatgtccag tagacccaaa caaagagaat ggggatcacc
     4741 gccagcacgc cgtaaatgag ctgatatgac gggaacatgg tgatataaag cgcgaaacct
     4801 ttctttcctg cttcgaacag gagtgcagcg acaaacgcgc cgacaatcgc gtcgcggtta
     4861 ggtacgcgga tggtaggaac aatgctgtac agcaaccaga aggagatcca cgacaacagc
     4921 agcggaaaaa tacgcagcac gttgtcgatg acagtattga gatcgctcgc ccagcgcagg
     4981 gagagcaaat aggaactgat cgccagactg gcccctgcca gcagcggccc cagcgttaaa
     5041 atcatccagt acacggcaaa cgagtaaatt tttggtcgcg ctcgtttact gcgccagatg
     5101 gtattcaacg cgctatcgat ggagtacatc aacaataatg cggtgacgat cagcccgcac
     5161 gcgccaacgg cggtcatctt gttggaattg gcaacaaatt gttcgatata ccgctgaata
     5221 acatcgccag tagcaggcag aaagttggca aaaataaagt gacgcaactg aatgctgacg
     5281 tcggaaaaca tgggaaaagc ggcgaaaagc gcaaaaacaa cggcaaccag cggcactaat
     5341 gagagcaaag acacataggc aaggttacct gccagggttg tcatgttgtc ctcatcaata
     5401 cgttgccaga gtagttttag ccaggcccat agtggacgag tacggtgcct ggctttgtcc
     5461 tgaatggttt ttagcataac accttcgcga aatagtccgg gatggtggtt ttatctttca
     5521 ccagaatact ggtaatgccc agctgattgg ctccttctat attatcggcg ttatcgtcga
     5581 aaaagaccgt atcgctgggt gaaaaacctt ccgcctgcaa aacatgctgg taaattcgtg
     5641 cttcaggttt gcgcatcccc agatcttgcg acagatagat atggtcagca gcatcacgaa
     5701 tttccgggta ttcttccggc cagaaggtgg tatgcaggcg gttggtattg gaaagcacca
     5761 ccacgcgatg cccctgctca cgcagtttat gcatgatggc gatcacttcc gggcgcagcg
     5821 caacaaacac cgcctgccag ccgtgagaga actgctcgta gcttagcggt agagccatct
     5881 catgacacag cgcctctgcg aacgcttcgt cgctaatttc cccacgctca tgctgatgaa
     5941 acgcctcccc catatgaaaa ctcttcttaa gcgatgccag cggaatacgc gttaaatcgc
     6001 tccaggctcc cagcacacgg ttaaagtcga tatcgacaat cacattacct aaatcaaaga
     6061 tatagagcat ttttgcctcc tttcgcgcca caagaaatta actgtagcgg gaaaggatgg
     6121 gtttggctat gtggcaaggt atgaagccag gttaacggtc acaaaaatct acgcgtcttc
     6181 ctggggaaga atgatttcaa tttggctgtc ttgtaagcgt gattttagcg cagcgggcgg
     6241 cagctcgtcg gtaaagagcg cagtgacctg tgccacgtta ccaatttcaa ccgccgccga
     6301 agcatgatac ttagtgtgat cggcgaccag cagaatattt ctcgcgtgcg ccatcatcgt
     6361 tttcaccacg ttagcttcgt ttacatcaaa ctccatcaac gcgccatcgc tctcaatcgc
     6421 cccaacgctt gttaccagat aatcagcacg aaaatcagcc acaaaggacg ccgctgaagg
     6481 gccaatgatc ccgctattat gagagcgcaa cgtaccgccg ggcaccatca cttcaaagcg
     6541 cgggttgtgg taaagaatat gcgccacacg caggctgttg gtgattatcc gcaaatgatt
     6601 atggttaagt aacgcccggg caacatgctc aacagtcgta ccaatggtga taaatattgt
     6661 tgaaccatca ggaatatagt ctgccaccgc ttcggcaatc gctttttttt cctcggtttg
     6721 cgaaacctca cgctgctcga acgccgtatt aacgacgctg gaagcccgac ccgcgccacc
     6781 gtgatggcgc gtaatcaggc cttgctcgct taatttacga atatcccggc ggaccgtctg
     6841 cgtggagaca tccagcagat ttgccagctc atcaatattc atatacccac gctcggcgat
     6901 cagcatgagg agttggtcgt gccgcgggtt accggttagt tcggtaaggc tcatgaaaat
     6961 tcctcgaaaa accatcaccc tggcatttta tacaaaaagt gacaaaaaag atcgggtttg
     7021 atcacagtca gggatcccgg ctcgtccacc gggacgtgtg cattttaacg ccgctacacc
     7081 gctggcgaag cggactgact ccgctaaatc cccacttgtt gccagcgcca ccgccaaagc
     7141 gccgtgaaaa acatcacccg cacctgtggt atctaccaca tcaactttga aggccggttg
     7201 atgctgacgc ccaccatttt ccagccagtc gcagcctgcg ctaccctggg tcacatagac
     7261 atgaccattt gtgagcgttt gtgcctgttt tagcgcactg gccatctctt tcacgcccgt
     7321 taagcgcgcc agacccggtt ctgaaaaggc cgcgtgatcg cttaatgcca ccagctcact
     7381 gatatcctgc ggcgtaatgt ccccgtccag aacggtcatc acacccgcct gacgggccag
     7441 ggtgaaggct tttttagcgc cgtcgtgcca gcgtacatct gccagcacaa catcccactg
     7501 agagaaatca atttcctcca accactctgc gtcaggcagc agatccgggc tggggtagtt
     7561 aatgattatc cgctcgcctt tggtatccac catgatggcg gattgcgaag atttcgcctg
     7621 gttataccgt ttggtgtaac gggtgttaac gccccaggat tccagctctg ccagcaggct
     7681 gttgcccgtg tcgtcatcac ctacgcgacc aataaaatcg acctgcgccc ccagccttgc
     7741 cgccgcaacc gctgccgtcg ccgctggccc gccaccaact tccgtataat ttctcgccac
     7801 gtatttaccg ctctccgtcg gtaacccttc cacgtaatag atgcgatcca tcacggttat
     7861 acctacacaa gcaacacgaa tcatggtcat tccttaaaca ttttgggatg cgttcatttt
     7921 aatttcacca attgtttaaa aagtgacggc tgtcaatttt ttgacgataa attacaaata
     7981 cgatcaaaaa cagacaagca aagcatgtca taaatgacaa aaagtgacat cactgtattc
     8041 aggagagggt tatggcagca atcgcgttta tcggtttagg acaaatgggt tcgccaatgg
     8101 cgagcaattt attgcagcaa gggcatcaac ttcgcgtctt tgatgtgaac gccgaagctg
     8161 tgcggcatct ggtagacaaa ggtgcgactc ccgccgccaa cccggcgcag gccgctaaag
     8221 atgccgaatt tatcattacc atgttgccga atggcgatct ggtgcgcaac gtgttgttcg
     8281 gtgaaaacgg cgtttgcgaa ggcttatcta ccgatgcgct ggtcattgat atgtccacca
     8341 tccatccgct gcaaaccgat aaattgattg ccgatatgca agccaaaggc ttcagcatga
     8401 tggatgttcc ggtaggccgt acttctgcaa atgccattac cggtactctg ttactgctgg
     8461 ctggcggcac cgctgaacaa gttgaacgtg ccacaccgat cctgatggcg atgggcagtg
     8521 agttgatcaa ctctggcggt ccgggcatgg ggatccgcgt taagctcatc aacaactaca
     8581 tgagcatcgc gctcaatgcg ctttcggcag aagccgccgt tttgtgcgaa gccctgaatc
     8641 ttcccttcga tgttgccgtc aaagtgatga gcggtaccgc cgccggtaaa ggtcacttca
     8701 ccacttcctg gccaaacaaa gtcctcagcg gagatctttc tcccgccttc atgatcgatc
     8761 ttgcccataa ggatcttggc atcgcccttg atgtcgccaa ccagctgcat gtgccaatgc
     8821 cgctgggggc cgcctcacgg gaggtttata gccaggcgcg cgcagcgggt cgcggtcgcc
     8881 aggactggtc cgccattctg gaacaggtcc gtgtcagtgc cgggatgact gccaaagtaa
     8941 aaatgtaacg actggataaa ggaataaatg aatgaataag tacaccatca acgacattac
     9001 gcgcgcatcg ggcggttttg ccatgctggc ggtcgatcag cgcgaagcca tgcgcatgat
     9061 gtttgccgcg gctggggcac ccgctccggt agccgatagc gttttaactg atttcaaagt
     9121 taacgctgca aaggccctct cgccttatgc ctcggcgatt ctggtagatc aacaattctg
     9181 ctaccgccag gtggttgagc aaaacgcgat tgccaaaagt tgcgccatga ttgtcgccgc
     9241 cgatgagttc attcctggca acggtattcc ggtcgatagc gtggttattg accgcaaaat
     9301 caatccgcta cagatcaaac aagacggtgg caaagcctta aaactgctgg tgctgtggcg
     9361 tagtgatgaa gatgcgcagc aacgtctgga tatggtgaaa gagttcaacg aactgtgcca
     9421 ctcacacggt ctggtaagca tcattgagcc agtcgtccgc ccaccgcgtc gtggcgataa
     9481 attcgatcgc gaacaagcga tcatcgatgc cgccaaagag ctgggcgaca gtggcgctga
     9541 cctctacaaa gttgaaatgc cgctttatgg caaaggtccg caacaagagc ttctctgtgc
     9601 ttcacaacgt ctgaatgacc atatcaatat gccatgggtg atcctctctt ccggtgtcga
     9661 cgaaaaactg ttcccgcgtg ccgtacgcgt ggcaatgacg gcgggcgcat cgggattcct
     9721 ggcaggccgt gcagtctggg catcggtcgt cggtttacca gacaacgagc tgatgctgcg
     9781 tgacgtttgc gcaccgaaat tacaacaact tggcgatatc gtcgacgaaa tgatggctaa
     9841 acgccgctaa gatgaggata aaaggaatga aatggtttaa caccctaagc cacaaccgtt
     9901 ggctggaaca ggaaaccgac cgcatctttg attttggtaa aaattccgta gtgccgactg
     9961 gttttggctg gttaggcaat aaagggcaaa tcaaagaaga gatgggcacc catctgtgga
    10021 tcaccgctcg tatgttgcac gtttattccg ttgctgcggc gatgggtcga cctggcgctt
    10081 actcgttggt tgatcacggt atcaaagcca tgaacggcgc actgcgtgat aaaaaatatg
    10141 gcggctggta tgcctgcgtg aatgacgagg gcgtggtgga tgcctccaaa cagggctatc
    10201 aacacttctt tgctctgctg ggtgccgcca gcgccgtcac aacgggtcac ccggaagcgc
    10261 gcaagctgct cgattacacc attgaaatta tcgagaaata tttctggagc gaagaagagc
    10321 agatgtgcct ggaatcctgg gacgaagcct tcagcaaaac cgaagagtac cgcggcggca
    10381 atgccaatat gcacgcggtg gaagctttct tgattgttta tgacgtcact cacgacaaaa
    10441 aatggctgga tcgcgcgatt cgcgtggctt ccgtgattat ccacgacgtc gccagaaata
    10501 atcattatcg cgttaacgaa cacttcgata cccagtggaa tccgctgccg gattacaaca
    10561 aagataaccc ggcgcatcgc ttccgcgcgt ttggaggtac accaggccac tggatcgaat
    10621 ggggccgttt aatgctgcac atccatgctg ccctggaagc ccgttgcgaa caaccaccag
    10681 catggctgct agaagatgcc aaaggtctgt ttaacgccac cgtgcgcgat gcctgggcac
    10741 ccgatggcgc ggacgggatt gtttataccg ttgactggga aggaaaaccg gtggtccgcg
    10801 aacgtgtacg ttggcctatc gtcgaagcaa tgggtacggc ctacgcgctc tacaccgtca
    10861 ctggcgatcg ccagtatgaa acctggtatc aaacatggtg ggagtactgc attaagtacc
    10921 tgatggacta tgaaaatggt tcctggtggc aggagctgga tgcggacaat aaggtcacca
    10981 ccaaagtctg ggacggcaaa caggatattt atcacctgct gcattgcctg gtgatcccgc
    11041 gtatcccgtt agcgcccggc atggctccag cggttgcagc gggtctgctg gatattaatg
    11101 cgaaataact gatctgaaaa tagggtgcgg gttttcccct caccctaacc ctctccccaa
    11161 aggggcgagg ggaccgtccg gtgcagtttt tgggtttgac atcagcctca cgggaggctg
    11221 tatgtcgtta attaaggtgc cttgtatgca aatcaccaat atgcattgca gtggacagac
    11281 cgtttcactt gccgcggggg attatcacgc gaccattgtg accgtcggtg ctggtctggc
    11341 tgaattaacc tttcagggtt gtcatttagt catcccgcac aaacccgaag agatgccgct
    11401 ggcgcatctg ggtaaagtgc ttattccgtg gccaaaccgg atcgccaacg gctgttaccg
    11461 ctatcagggg caggaatatc agttacccat taatgaacat agttcaaaag ccgctattca
    11521 cgggctgctt gcctggcgag actggcaaat tagtgaatta accgccacca gcgttaccct
    11581 gacagctttt ctgccgccca gttacggcta tccgtttatg ctggcttcac aagttgttta
    11641 ttcgctcaac gcacacacgg gtctttccgt tgagatcgca agccagaata tcggcactgt
    11701 cgcagcgcct tatggcgtgg gtattcatac ttatttaacc tgcaacttaa catctgttga
    11761 tgaatatctg ttccagcttc cggcaaacca ggtttatgcc gttgatgaac acgtaaatcc
    11821 gacaacgctt caccacgttg atgagctgga tttaaatttc acgcaggcga aaacaatagc
    11881 cgcgacaaaa atcgaccaca cctttaaaac cgctaatgat ctgtgggaaa taaccattac
    11941 ccatccacaa caggcgcttt cggttagcct ttgttgcgat caaccctggg tacaaattta
    12001 tagcggtgaa aaattacagc gtcagggact ggcagttgaa cctatgagtt gcccgcctaa
    12061 cgcctttaat tcaggtatcg acttattatt actggaacca ggaaaaccgc accgcctgtt
    12121 ttttaacatt tacggacaga gaaaataact tgtattgatt agcagtcaaa atcattgaag
    12181 aacatttcac tcgctaaagg caacattatg tatcttcaat ttattaagtt aaaaacagct
    12241 cagcttctgt aaagttttta tatatcagtt ttatgagaat cattttacat aagcctgtaa
    12301 taacaggaag gcaacaatct cgttctcaac tctatggaga ttagttatgg atacgccacg
    12361 tccacagtta ttagattttc aatttcatca gaataacgac agttttaccc tacattttca
    12421 acaacgtctt attttaaccc atagcaaaga taatccttgt ttatggattg gctcaggtat
    12481 agcggatatc gatatgttcc gcggtaattt cagcattaaa gataaactac aggagaaaat
    12541 tgcgcttacc gacgccatcg tcagccagtc accggatggt tggttaattc atttcagccg
    12601 tggttctgac attagcgcca cgctgaatat ctctgccgac gatcagggcc gtttattgct
    12661 ggaactacaa aacgacaacc ttaaccacaa ccgtatctgg ctgcgccttg ccgctcaacc
    12721 agaggaccat atctacggct gcggcgaaca gttttcctac ttcgatctgc gtggcaaacc
    12781 gttcccgcta tggaccagtg aacaaggcgt tggtcgcaac aaacaaacct atgtcacctg
    12841 gcaggccgac tgcaaagaaa atgcgggcgg cgactattac tggactttct tcccacagcc
    12901 tacgtttgtc agcacgcaga agtattactg ccatgttgat aacagttgct atatgaactt
    12961 cgactttagt gccccggaat accatgaact ggcgctgtgg gaagacaaag caacgctgcg
    13021 ttttgaatgt gctgacacat acatttccct gctggaaaaa ttaaccgccc tgctgggacg
    13081 ccagccagaa ctgcccgact ggatttatga cggagtaacg ctcggcattc agggcgggac
    13141 ggaagtgtgc cagaagaaac tggacaccat gcgtaacgcg ggcgtgaagg tcaacggcat
    13201 ctgggcgcag gactggtccg gtattcgtat gacctctttt ggcaaacgcg tgatgtggaa
    13261 ctggaagtgg aacagcgaaa actacccgca actggattca cgcattaagc agtggaatca
    13321 ggagggcgtg cagttcctgg cctatatcaa cccgtatgtt gccagcgata aagatctctg
    13381 cgaagaagcg gcacaacacg gctatctggc aaaagatgcc tctggcggtg actatctggt
    13441 ggagtttggc gagttttacg gcggcgttgt cgatctcact aatccagaag cctacgcctg
    13501 gttcaaggaa gtgatcaaaa agaacatgat tgaactcggc tgcggcggct ggatggctga
    13561 cttcggcgag tatctgccca ccgacactta cctgcataac ggcatcagcg ccgaaattat
    13621 gcataacgcc tggcccgcgc tgtgggcgaa atgtaactac gaagcccttg aagaaacggg
    13681 caagctcggc gagatccttt tctttatgcg cgccggttct accggtagcc agaaatactc
    13741 caccatgatg tgggcgggcg accagaacgt cgactggagt ctcgacgatg gcctggcgtc
    13801 ggttgtcccg gcggcgctgt cgctggcaat gaccggacat ggcctgcacc acagcgacat
    13861 tggcggttac accaccctgt ttgagatgaa gcgcagcaaa gagctgctgc tgcgctggtg
    13921 cgatttcagc gccttcacgc cgatgatgcg cacccacgaa ggtaaccgtc ctggcgacaa
    13981 ctggcagttt gacggcgacg cagaaactat cgcccatttt gcccgtatga cctccgtctt
    14041 caccaccctg aaaccttacc tgaaagaggc tgtcgcgctg aatgcgaagt ccggcctgcc
    14101 ggttatgcgc ccgctgttcc tgcattacga agacgatgcg cacacttaca ccctgaaata
    14161 tcagtacctg ttaggtcgcg acattctgat cgctccggtg catgaagaag gccgtagcga
    14221 ctggacgctc tatctgccgg aggataactg ggtccacgcc tggacgggtg aagcgttccg
    14281 gggcggggaa gttaccgtta atgcgcccat cggcaagccg ccggtctttt atcgcgccga
    14341 tagcgaatgg gcggcactgt tcgcgtcgtt aaaaagcatc taatcacatc cgcccgtggg
    14401 gaaactcatg ggcaaaggga gaacatcgat gagtcacatc acaacggaag atccagcaac
    14461 tttacgcctg ccctttaaag agaaactctc ttacggtatt ggcgacctgg cctctaacat
    14521 cctgctggat atcggtacac tttatctttt gaagttttat accgacgttc tggggctgcc
    14581 aggcacctat ggcggcatta tctttttgat ttcaaaattc tttactgcgt ttaccgatat
    14641 gggtaccggc attatgttgg attcccgacg caagatcggt ccaaaaggta agttccgtcc
    14701 ttttattctg tatgcgtcat tcccggtcac cttactggcg atcgccaact ttgtcggcac
    14761 accgtttgat gtcaccggta aaacggtgat ggccactatt ctgtttatgc tttacggact
    14821 gtttttcagc atgatgaact gctcctacgg cgcaatggtt cccgctatca ccaaaaaccc
    14881 caacgaacgc gcctcactgg cggcatggcg tcagggcggc gctacgctgg gcctgctgct
    14941 gtgcacggtg ggattcgtgc cagtgatgaa tcttatcgag ggtaatcagc aacttggcta
    15001 tatcttcgcc gccacgctgt tttcactgtt cggcctgctg tttatgtgga tctgctactc
    15061 aggcgtgaaa gagcgttatg tcgaaaccca accaaccaat ccggcgcaaa agcctggcct
    15121 gttgcaatct ttccgcgcaa ttgccggtaa ccgcccactg ttcattctgt gtattgccaa
    15181 cctctgcacc ttaggggcgt ttaacgtcaa gctcgccatt caggtctatt acacccagta
    15241 cgtacttaac gatcccatcc tgttgtcgta tatgggattt ttcagcatgg gctgtatttt
    15301 catcggcgtg ttcctgatgc ctggcgcagt cagacgtttt ggtaagaaga aggtctatat
    15361 cggcggcctg ctgatttggg tgctgggcga tctgctcaac tatttcttcg gcggcggctc
    15421 ggtcagcttt gtggcgttct cctgcctggc gttcttcggc tcagcgtttg ttaacagcct
    15481 gaactgggcg ctggtttccg acaccgtcga gtacggcgag tggcgcaccg gcgtgcgttc
    15541 ggaaggcacg gtctacaccg gcttcacctt ctttcgcaaa gtgtctcagg cgctggctgg
    15601 tttcttcccc ggctggatgc tgacgcaaat tggctatgtg ccgaacgtcg cacaggctga
    15661 ccacaccatt gaagggttac gccagttgat cttcatctac ccaagcgcac tggcggtagt
    15721 caccattgtg gcgatgggtt gcttctacag cctgaacgag aagatgtatg tccgcattgt
    15781 ggaagagata gaagcccgta aacgcacggc gtaataataa taaacgacgc cctgcggggc
    15841 gttataagga gtgattatgt ctgaccataa tccactgaca ttaaaactga atctgcggga
    15901 aaaaatcgcc tatggtatgg gcgacgtcgg ttcgaattta atgctctgca tcggtactct
    15961 gtatctcctc aaattttata ccgatgaatt agggatgcct gcttactatg gcgggattat
    16021 ctttctggtg gcgaagtttt ttaccgcgtt taccgatatg ctcaccggat ttttactcga
    16081 ctcgcggaaa aatattgggc ctaaaggcaa atttcgtccc tttattttat atgcagcagt
    16141 tccggcggcg ttaattgcaa cgctccagtt tatcgccacc accttttgtt tgccggttaa
    16201 aacgacgatt gccaccgcac tgtttatgat gtttggactt tcatacagtc tgatgaactg
    16261 ctcgtatggt gcgatgatcc cggcaattac caaaaacccg aatgagcgcg cgcagctcgc
    16321 ggcttaccgc cagggtggtg caaccatagg gctattgatt tgtaccgtgg cgtttattcc
    16381 gttgcagtcg cttttttctg actcaaccgt cggttatgcc tgtgcggcac ttatgttctc
    16441 cattggcggc tttattttta tgatgctgtg ctacagaggc gtcaaagagc attatgtgga
    16501 cacagcgcca accggacata aagccagtat tctcaaatct ttttgcgcga tatttcgtaa
    16561 tccaccattg ctggttttat gcattgctaa cctgtgtact ctggcggcat ttaatatcaa
    16621 actggcgatt caggtctatt acacccagta tgtgctgaat gatattaatt tattgtcgtg
    16681 gatgggattc ttcagtatgg gatgcatcct cgtcggcgta ttactggtgc cagtaactgt
    16741 aaaatgtttt ggtaaaaaac aggtttattt agctggcatg gtgctgtggg cggtgggtga
    16801 tatactgaat tatttctggg gaagtaactc tttcactttc gtcatgttct cttgtgtcgc
    16861 cttttttggc acggcctttg taaacagcct gaactgggca ctggtaccag ataccgtaga
    16921 ttacggtgaa tggaaaaccg gtattcgtgc cgaaggttct gtttataccg gttatacctt
    16981 ctttcgtaaa atttctgccg cacttgctgg cttcttgcca ggcattatgc tgacgcaaat
    17041 tggttatgtt cccaacatcg cgcaaagcga tgcgacatta cagggtttgc gtcagctcat
    17101 ttttatctgg ccttgtgcac tggcaattat tgctgcattg acgatgggat tcttttacac
    17161 actcaatgaa aaacggtttg cattgattat tgaggaaatc aaccaacgca aaaataaaga
    17221 gatggcgaca gaagaaaaaa cggcttccgt aacgttataa atgtcaatag ccgctatttc
    17281 cattctggtg ggtggcggcc tccctacgtt taaaaaatgg acttattatg aaaaagatta
    17341 atgcaataat tttattatct tctctgacct ctgcctcggt atttgctggc gcatacgtcg
    17401 aaaacaggga ggcatataat cttgcctccg atcagggcga ggttatgcta cgtgtgggtt
    17461 ataacttcga tatgggcgcg ggtattatgt taaccaatac ctacaccttt cagcgagaag
    17521 atgaactaaa acatggctat aatgaaattg aaggttggta tcctttattc aaaccaaccg
    17581 ataaattaac catccagccc ggtggcttaa ttaatgataa gagtatcggt tcgggtggtg
    17641 cagtgtatct ggacgtcaac tataaatttg taccatggtt taatctgaca gtacgaaatc
    17701 gctataacca taataactat agttcaacag atttgagcgg ggaactggat aataatgaca
    17761 cctatgaaat tggcacctac tggaatttta aaatcaccga taaattttcc tatacatttg
    17821 agccacatta tttcatacgc gtaaatgact ttaatagcag caatggaaaa gatcatcact
    17881 gggaaatcac taataccttc cgttatcgca ttaatgaaca ctggctgcct tatttcgaat
    17941 tacgttggtt agatcgtaat gtcgaaccgt accaccgcga gcaaaaccag attcgtatcg
    18001 ggacgaagta tttcttctga tgtgaaactg ccggatgcgg cgtaaacgcc ttatccggcc
    18061 tacggttgat acggttttcg ttggccggat taagcacatc cggccattaa tcaagcttca
    18121 gccgccagca tctctttctt acgttggcta attcgcttaa ccagcattac ggaaaccacc
    18181 gcaccacaga atgcgaagca ggccataatg ccaaacacga ttttgtagcc aataatcccc
    18241 ggatttaaat ccagaatgta gccatacaga ctgaagcaga acatcgccgg agcgtaacca
    18301 ataaagctac ccaacgccat cgccgcgcct gttttatttt cagcaatttt tgcttcgccg
    18361 ataggtgcaa aaaatacggc tcgctgtgta aagactatcg cgccaaagcc cagcgtacat
    18421 gccatcccta aatagaccgg catactttcg tgcggcagca taatcaacag tacgagcgca
    18481 gcggtactga tgataaaggt gtagcataga tatttactcg gcgatttcag gatcttatct
    18541 gaaatcatgc caccaatcgg tccaccaatc attttcagac agtattggtt aatgatgccg
    18601 taagccccca ccagcgcaac gggcaataga tagatgtttt tcaggaatgg aatgaagaat
    18661 gtcaggccac agtaaaccgc gtaaacgaag aagacgttaa aagcaataag ccagatagtt
    18721 ttgtctttca gcaccgaggt catactggtg tttttcgatg ctccgtcttc ttttttcacc
    18781 tcgacggacg gtgcctcttc tttgtcattc agaacaaaga aaatgataat gcctacggca
    18841 atcacaataa gggaatagaa ccagatgcct gctttaaaac ccaataagcc actgccaaac
    18901 caggtaaata ctgccaacgc agaaaatgcc accacggtat cgacaatgcc acgccctgtt
    18961 tcgaagaagc caaacaaccg accttgttgt tcgctattgc ccaatcgact caccgacttg
    19021 agcaagaccg gccagttcat catgtcgcaa gtaacgccaa atagcgccca gacaaagagg
    19081 atgccccaat accccggcat tgtcgtcaaa taaacaccca gtaatcctgt cgcaatgagt
    19141 gagaatgaca tggtgtatct gcgcggtagt ttatcggcaa aataaataga cagaaaaaaa
    19201 cccactgtcg tgacaaatga gtttaccgac atagcattac caatttgacc attggtcaaa
    19261 tggaaatatt cctgcatcgg gatataaaac gcatctttca gcgacggtaa tttataaatt
    19321 gtaccgccac acaatgttaa tagactaaat aacgcccatt tctttttcgt gagcataaca
    19381 tcccctaagc gatttttaaa atcaatgttt taaaggcaat aagataccga ccattcatat
    19441 ggaatggttt tattaactgc gtatgagtta aaacttatta gtggttcaga agatggtttg
    19501 cctcttgttt gatttgttgt agtacgttac gcacatgatt tatctgatta attgcatctt
    19561 ctttttcttt acgtaaccgc gcatccagta aatgattttc tggcaatggt gtttcactca
    19621 tctggcgata agggatccac ggattatcat cttcgtcttc aaagcggaac gccggcgcat
    19681 agtaatcaac ctcttcttcc aggccatatg ccgtcacctg cggctcatca tcacccaggc
    19741 atataaggtg cgttaataac gctttgaacg gcatatcacc ctgacctgaa atacaggctt
    19801 tatgacccag gccacccggt tctttaacga tcaaggcatc tttgatatgg acctgggtaa
    19861 tatgcggcgc catcgttttt aaagcgtcga tgggatgctc atttgcattg atcatgttcg
    19921 caaaatcaaa cagtaaggaa agagattcca tctcactttc tttgaccagc gacaccagct
    19981 catggctttt taaatcttca tgctgttcga tcgtaaaagt caggccgctg tcctgatacg
    20041 tttcccgtac ataggcaatg tcgttagcga tgatcgataa tacgtcgcgc aaattacctt
    20101 cataacgtgg ataaaaacgt acggacgatg ccccagtttt caacgcaatg gcgacggctt
    20161 cgtcaataga tgccttatct gaagcgctgg tttcaatatg aatatcaagg ttcagacggc
    20221 gggctttatc cccaaaggca gagagttctt tatcgtccat attaccaaga gaaaaacgct
    20281 cgccatccag aacatggatt ttgacgccac gtagattatt ttcacttgcg atatcaagta
    20341 aatcagcagg caacactctt tccagccgca tatttaaatg aaaggcataa gcatgtaaat
    20401 aaagaggaag agtgtcgaca cgttgtagaa tctttcttgc gttatttatt gtcaccataa
    20461 aacacctaca gattaatgat gaggtctacg gcctcaaatc gccaatattc aatatctatc
    20521 tggacaatat tgccttgctg gtcggcgcga tgcttttcaa ttaagatcgc tggcataccc
    20581 gcagaaccgc caagatactt gctgatatcg cccggcattc tgacgggttt gaaaatcaat
    20641 ttttttacta ccgtctcttt ttggtagcac ttttcccaga ctgcagaaaa tgagtggttt
    20701 tcaagttgtt caataaaacc cggagcaact tcaggattaa tatatgtttc gtgatagaaa
    20761 actttatgtc cttccagcgc cccccagccg gtgatgcgat agagttcagt tgatggtgtc
    20821 actgcaatca gtggcgcgag cgtctcggga atatcgctgg tacggttttt ctcggtaaac
    20881 ccccaggaag gttcccgccc ttgctcaatt gcggcccgct gaaagctcgc cgacagctcc
    20941 gggctgtaat taaatcgcgg ctgagtcacg aaccaccctt tacgatcctt acggaagatt
    21001 ttggattctg cctcgaggtt cagcaacgcc tggcgcagcg tcatacgttt aatgcccagc
    21061 agctctccca gctctctttc tgaagggagt tttccacccg gcgtagtgat gccatctttt
    21121 aaccaccgat ccagtttctc ttttgcattt tctacggtgg attgatttcc cgtcattgtt
    21181 taatcctttt ggtttaaacc agatgaggca tcttacgctt acgccaaaaa agaaagcaat
    21241 taacggcgta tgactttgca gataattgtg acaaagctct ctattgacgt aaccacagac
    21301 ttataaggga gatactcgca ttgctgagtg ctcacgacag gcaaaaaaaa acctgccatc
    21361 gctggcaggt tttttatgac taaaaaacga aattaatcgt ctttcggtgc gcggttggcg
    21421 cgacgacgat cgttttccgt caggtgacgt ttacgaatac ggatagaggt cggggtcact
    21481 tctaccagtt cgtcatcatc gatgaactcc agagcttgtt ccagagtcat gcggataggc
    21541 ggaaccagaa caacggcttc gtcagtaccg gaagcacgca tgttggtcag tttcttaccg
    21601 gtcaggcagt ttacagtcag gtcgttagag cggctatgaa taccgataat ctgaccttcg
    21661 taaacttctg caccgtgacc gaggaacagc ttaccgcgat cctgcagacc gaacagcgcg
    21721 aacgcgaccg ctttaccctg accgttagag atcagtacgc cgttctgacg ctgacccact
    21781 tcacccggac gtacgtcgtc gtagtggctg aaggtggagt acagcagacc agtaccggaa
    21841 gtcatggtca tgaactcaga acggaagcca atcagaccac ggcttgggat cacgtagtcg
    21901 agacgtacgc ggcctttacc gtctggattc atgtttttca ggtcgccttt acgttcgccc
    21961 agcgcctgca ttacagaacc ctgatgctgt tcttcaacgt ccagcgtcac gttttcatac
    22021 ggctcttgtt tacgaccgtc gatttcacgg aagataactt tcggacggga taccgccagt
    22081 tcgaaacctt cacgacgcat gttttcgatc agaacagaca ggtgcagttc gccacgacca
    22141 gaaacgcgga acgcatcggc gtcttcggtt tcttctacgc gcagcgcaac gttatgtacc
    22201 agttctttgt tcagacgatc caggatctga cgagacgtta cgaacttacc ttctttaccg
    22261 cagaacggcg aggtgttaac gcagaagaac atagaaacgg tcggctcatc aacggagagt
    22321 gccggcagcg cttcaacgtt ttgcgtgtcg caaacggtgt cagaaatgtt cagttcgcca
    22381 aggcccgtga tcgccacgat atcgccagct tccgccagat cggtttcgat acgttccaga
    22441 ccgaggtggc ccagcacttt accgactttc gcgttgcggg ttttgccttc gctatcgatg
    22501 atagtgacct gttggttcgg cttcacttta ccgcgcttga tgcggccaat gccgataacg
    22561 ccaacatagc tgttgtaatc gagctgagaa atctgcatct ggaacggacc gtcaaggtca
    22621 acgtccggcg caggaacgtg atcaacaatc gcctgataca gcggggtcat gtcttccgcc
    22681 atatcttcgt ggtccagacc cgcgataccg ttcagcgcag aagcgtaaac gatcgggaag
    22741 tccagctgct cgtcggtcgc gtcgaggtta acgaacagat cgaatacctg atccacaacc
    22801 caatcaggac gcgcgccagg gcggtcaact ttgttgataa caacaatcgg cttcaggccg
    22861 taagcaaacg cttttttggt tacgaagcgc gtttgcggca tcgggccgtc aaatgcgtca
    22921 accaccagca gcactgagtc taccatggac attacacgtt caacttcacc accgaagtcg
    22981 gcgtgccccg gggtatcaac gatgttgata cggtaatcat tccatttgat agcggtgttt
    23041 ttcgcgagga tggtaatccc acgctctttc tccaaatcgt tggagtccat cacgcgctct
    23101 tgggtttcgg cacgagagtc gaacgtaccg gattgttgga gcagcttgtc taccagggtg
    23161 gttttaccat ggtctacgtg cgcgatgatg gcgatattac gcaatttttc gatcacaact
    23221 ttgcctcagg cattagaaat agcgcgttat tgtacacgga ttaatcgcac tacaaaacag
    23281 gatcacaaac atcctccgca aacaagtatt gcagagtccc tttgtgatcg ctttcacgga
    23341 gcataaaaag ggttatccaa aggtcattgc accaacatgg tgcttaatgt ttccattgaa
    23401 gcactatatt ggtgcaacat tcacatcgtg gtgcagccct tttgcacgat ggtgcgcatg
    23461 ataacgcctt ttaggggcaa tttaaaagtt ggcacagatt tcgctttatc ttttttacgg
    23521 cgacacggcc aaaataattg cagatttcgt taccacgacg accatgacca atccaggaga
    23581 gttaaagtat gtccgctgaa cacgtactga cgatgctgaa cgagcacgaa gtgaagtttg
    23641 ttgatttgcg cttcaccgat actaaaggta aagaacagca cgtcactatc cctgctcatc
    23701 aggtgaatgc tgaattcttc gaagaaggca aaatgtttga cggctcctcg attggcggct
    23761 ggaaaggcat taacgagtcc gacatggtgc tgatgccaga cgcatccacc gcagtgattg
    23821 acccgttctt cgccgactcc accctgatta tccgttgcga catccttgaa cctggcaccc
    23881 tgcaaggcta tgaccgtgac ccgcgctcca ttgcgaagcg cgccgaagat tacctgcgtt
    23941 ccactggcat tgccgacacc gtactgttcg ggccagaacc tgaattcttc ctgttcgatg
    24001 acatccgttt cggatcatct atctccggtt cccacgttgc tatcgacgat atcgaaggcg
    24061 catggaactc ctccacccaa tacgaaggtg gtaacaaagg tcaccgtccg gcagtgaaag
    24121 gcggttactt cccggttcca ccggtagact cggctcagga tattcgttct gaaatgtgtc
    24181 tggtgatgga acagatgggt ctggtggttg aagcccatca ccacgaagta gcgactgctg
    24241 gtcagaacga agtggctacc cgcttcaata ccatgaccaa aaaagctgac gaaattcaga
    24301 tctacaaata tgttgtgcac aacgtagcgc accgcttcgg taaaaccgcg acctttatgc
    24361 caaaaccgat gttcggtgat aacggctccg gtatgcactg ccacatgtct ctgtctaaaa
    24421 acggcgttaa cctgttcgca ggcgacaaat acgcaggtct gtctgagcag gcgctgtact
    24481 acattggcgg cgtaatcaaa cacgctaaag cgattaacgc cctggcaaac ccgaccacca
    24541 actcttataa gcgtctggtc ccgggctatg aagcaccggt aatgctggct tactctgcgc
    24601 gtaaccgttc tgcgtctatc cgtattccgg tggtttcttc tccgaaagca cgtcgtatcg
    24661 aagtacgttt cccggatccg gcagctaacc cgtacctgtg ctttgctgcc ctgctgatgg
    24721 ccggtcttga tggtatcaag aacaagatcc atccgggcga agccatggac aaaaacctgt
    24781 atgacctgcc gccagaagaa gcgaaagaga tcccacaggt tgcaggctct ctggaagaag
    24841 cactgaacga actggatctg gaccgcgagt tcctgaaagc cggtggcgtg ttcactgacg
    24901 aagcaattga tgcgtacatc gctctgcgtc gcgaagaaga tgaccgcgtg cgtatgactc
    24961 cgcatccggt agagtttgag ctgtactaca gcgtctaata gttgaagttg tactacccgg
    25021 cgcaacaacg ccgggattta gttgccgtgg aaactttcag cccatctctg catgggcttt
    25081 tttctccgtc aattctctga tgcttcgcgc tttttatccg taaaaagcta taatgcacta
    25141 aaatggtgca acctgttcag gagactgctt tatggcaaca ggcacgcagc ccgatgctgg
    25201 gcagatcctc aactcgctga ttaacagtat tttgttaatc gatgacaacc tggcgatcca
    25261 ttacgccaac cctgccgcgc aacaactgct cgcccaaagc tcccgcaaat tgtttggtac
    25321 accgttaccg gaactgttga gctacttctc attaaatatc gagctgatgc aagaaagtct
    25381 ggaggcgggg caaggtttta ccgataacga agtgacgctg gtcatcgacg ggcgctcgca
    25441 tatcctttct gtgacggccc agcgtatgcc ggacggcatg atcctgctgg agatggctcc
    25501 gatggataac cagcgccgct taagtcagga acagctacag cacgcccagc aggttgctgc
    25561 ccgtgattta gtgcgcggcc tggcacatga gattaaaaat ccgcttggcg gtttacgtgg
    25621 cgcggcgcag ctactcagca aagcgttacc tgacccatca ctactcgaat ataccaaagt
    25681 gattatcgaa caggcggacc ggctgcgaaa tctggtcgac cgtctgttgg ggccgcagct
    25741 gcccggtacg cgcgttaccg aaagtattca caaagtagct gaacgcgtgg taacgctggt
    25801 gtcgatggaa ctgccggaca acgtgcggtt gattcgtgat tacgatccca gcctgccgga
    25861 actggcgcac gacccggatc aaattgaaca ggtcttgctg aatattgtgc gcaatgcgct
    25921 acaggcgctg gggccggagg gtggtgaaat cattctgcgt acccgcaccg cgtttcaact
    25981 gaccttacac ggcgagcgct accggctggc ggcgcggatt gatgtggaag ataacgggcc
    26041 gggcattccg cctcatttgc aggatacgct gttttacccg atggtcagcg gccgcgaagg
    26101 tggcaccggg cttggcttat ccatcgcccg taatttgatt gatcagcatt caggcaaaat
    26161 tgaatttacc agttggccag gtcataccga gttctcggtt tacctgccta tcaggaaata
    26221 aaggtgacgt ttatgcaacg agggatagtc tgggtagtcg atgacgatag ttccatccgt
    26281 tgggtgcttg aacgtgcgct cgctggagcg ggtttaacct gtacgacatt tgagaacggc
    26341 gcggaagtac tggaggcgct ggcgagcaaa acgccggatg tgctgctttc agatatccgt
    26401 atgccgggaa tggacgggct ggcgctgctc aagcagatta aacagcgcca tccaatgctt
    26461 ccggtcatca ttatgaccgc acattccgat ctggatgctg ccgtcagcgc ctatcaacaa
    26521 ggggcgtttg attatctgcc caaaccgttt gatatcgacg aagccgtcgc gctggttgag
    26581 cgcgccatca gtcattacca ggaacagcag cagccgcgta atattcagct taacggccca
    26641 acgaccgata tcatcggcga agcgccagcc atgcaggacg tgttccgtat tatcggtcgg
    26701 ctttcgcgtt cttctattag cgtgctgatt aacggcgaat ccggcaccgg taaagaactg
    26761 gtcgctcatg ccctgcatcg ccacagtccg cgcgccaaag cgccgtttat cgcgctgaat
    26821 atggcagcta tcccaaaaga tttgatcgaa tcagaactgt ttggccacga gaaaggcgcg
    26881 tttactggcg cgaataccat tcgtcagggg cgttttgaac aggccgatgg cggtacatta
    26941 ttcctcgacg aaattggtga tatgccgctg gatgtgcaga cgcgtttgct gcgcgtgctg
    27001 gcagacggtc agttttaccg cgttggcggc tatgcgccgg tgaaagtgga tgtgcggatt
    27061 atcgctgcca ctcaccagaa tctcgaacag cgagtgcagg aaggtaagtt ccgtgaggat
    27121 ctgttccacc gcctgaacgt tatccgcgtt catctgccgc cgctgcgcga acgtcgggaa
    27181 gatattcccc gtctggcgcg ccatttttta caggttgccg cgcgcgaact gggcgtagaa
    27241 gcgaagttac tgcatccgga aaccgaagct gctctgacgc gtctggcgtg gccaggcaac
    27301 gtgcgccagc tggaaaacac ctgccgctgg ctaacggtga tggccgccgg gcaggaagtg
    27361 ttgattcagg atttgcccgg cgaactgttt gaatcaacgg ttgcggagag tacttcgcaa
    27421 atgcaaccgg acagctgggc gacgcttctt gcgcagtggg cagacagagc gctgcgttcc
    27481 ggtcatcaaa atctgctttc cgaagcgcag ccagagctgg agcggacgtt actgacgacc
    27541 gcgttgcgac atacgcaggg gcataaacag gaagcggcgc ggctactcgg ctggggccgc
    27601 aacaccctga cgcgtaagtt aaaagagctg gggatggagt gattcacagc ttgtgtgtaa
    27661 agattgatta ttgagcgcaa attgctggta ttttacgctt tactgttccg ataagttcag
    27721 tatgatcttg cccggaaaac ggggagagtc attatgctgg aatcaataat taatctggta
    27781 tcgagtggcg cagttgacag ccacacaccg caaactgccg ttgctgccgt gctgtgtgcc
    27841 gcaatgattg ggctgtttag ttgagtttca gattgctgat gaagtgctct ttgttcatgc
    27901 cggatgcggc gtgaacgcct tatccggcct acaaaagcaa tattttcaac aggttttgta
    27961 ggcctgataa gcgtagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt
    28021 tttctacttt gtaaacgaag cgccattcac tatttaaatc acccgtgaga actgctgcat
    28081 ccgcgctttc tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg
    28141 acctttcgcc gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa
    28201 cggggcgagc agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt
    28261 ctcaataggg gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg
    28321 aatacagtca tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatccac
    28381 ttgctgatag tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct
    28441 gatggcggaa acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa
    28501 gttacgatgc agcacgcctt cacgctgggc caccgccagc tcgtcatccg gacgggcaaa
    28561 gtgatccata ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag
    28621 gatatcgagt ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc
    28681 aaaaatggtc ggcagatgcg cgtagttaaa gacactcaga cgatcggggt tcagctccgc
    28741 cacacgtttc agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag
    28801 gtcgatgttg gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa
    28861 ctcttcatcc tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc
    28921 catgctcagg cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc
    28981 ccgcggatcg acttcgatcg aaatctccgc atcggcattg aactggaagt tttcgcgcag
    29041 cagcttcatc aggcggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca
    29101 gtgcaattgg ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc
    29161 cagcgcgtcc agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc
    29221 gcagaagtaa caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc
    29281 aggatagcgc gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag
    29341 cgcggtcggg tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag
    29401 atcccagtcg atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta
    29461 cggcgtaccg gcggatgccc gtttcgggtc attagccggt tgcgcagcca cttttgtcgc
    29521 cgcgacaacc gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt
    29581 atcagcagaa cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc
    29641 atgtcttctt gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt
    29701 ttctgcatca actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggca
    29761 ctcagggttt cgcctgcttc cagacgttcc agcagcgcat ccagacgctc atccgtttcc
    29821 agtaattcca actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt
    29881 ttggtgactt tttcagtcac gcccaatgga ataggggttt tactgccaat acgtggatct
    29941 tttggtgcgt tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc
    30001 ggcgcatgac cacgacgttt tttctgacgc ttgcgatcac gagcttcctg atccagctcc
    30061 tcgcgtgttt ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt
    30121 ttcataatgg ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca
    30181 gaaagcaagc aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa
    30241 cccttaacgg gtcttacaat ccttgcaggc cgccaacaca ccctgttttt cctttagccg
    30301 gaacaatccg tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga
    30361 caatcctctg tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt
    30421 agcgtcctcg ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc
    30481 atcagtattt gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt
    30541 atttgacatg catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct
    30601 cacaaagcgt atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg
    30661 ttcaggtata atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa
    30721 ttatcaacag acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac
    30781 cggaattgaa gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac
    30841 gctgactaac cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat
    30901 caacctgttt gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc
    30961 ggaagtcccg gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa
    31021 acgtcagagc ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt
    31081 ggatcagcag atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac
    31141 caaagcggac aaactggcaa gcggcgcacg taaagcgcaa ttgaatatgg tgcgtgaagc
    31201 tgtactggcg tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg
    31261 cgtggacaag ctgcggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga
    31321 aacgcaggac ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg
    31381 cccgtaaatt caatagatta caattctacc gttggcctga taaacatagc gcatcaggca
    31441 ttacggatct tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct
    31501 aaaatattca gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac
    31561 ctctgtaccc tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt
    31621 aatttttttt cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta
    31681 tgttgcttac ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt
    31741 tttcgccact ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca
    31801 gttgatgaat ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt
    31861 catcgtgtac ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa
    31921 cggcaatcat cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg
    31981 ctgcacgttc agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca
    32041 gacggcgtcc gtccagcgtt tcaacgtagc cctgctcttt cgcctgagca cgggtgcgtt
    32101 ccatatactc cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt
    32161 ctttacgtgg aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac
    32221 caaagttgat cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac
    32281 caaacacttc tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca
    32341 agcctttgtc acgcgaaaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg
    32401 agacaatcac ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt
    32461 tacgcaccgg aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta
    32521 ctgcctggtg ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct
    32581 tgtcggtgta ggtcgatttc agcttcgcca gaccacgata ctccagaatc acttttggca
    32641 acggatagtc cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg
    32701 gcgttttctt cagcggttta ataccctgtt tttcaaagag aatggtttgc aactgcttgg
    32761 tggaagaaag gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca
    32821 gacgcaaggt gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt
    32881 tacgttcaat gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca
    32941 acggcccttt gtgtttttgc agatctggcc acattttcag atgcaactgc aaggtgacat
    33001 cagcatcttc ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt
    33061 gatttttgcc tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt
    33121 ccgcgaggct gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca
    33181 tggtatcaaa cgcaatccca cgcagctcaa tgccgtaatt cgccagaata ccgcgatcgt
    33241 atttcaggtt ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact
    33301 cgagtgcacg ctcgcgagag atttgatcgg gcgcgtcaag ataatcatga gcaaccggaa
    33361 tatatgccgc tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt
    33421 tatcaaggct gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa
    33481 tccacgcttt cagtgtttct tcatcaagga tggtgacgta gttgtcataa gaaatcaccg
    33541 ttgccgtcac ttctggcgct tcgtctgcaa cactggtttc ctgtggcttc gcggctggtt
    33601 ttgccccttt ggcctgtaac catttgcccg cttcgacatc agcagtccag cgtttgaact
    33661 catacttttt gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt
    33721 cacaggtcag ctccagttca acgtcggttt taatcgtcgc cagctggtat gagagataag
    33781 caacttcttt gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc
    33841 cagcaatttt ttctggctcg gcatacagcg tatccagtcc gccaagacct tgcagcaatg
    33901 cctgcgcggt tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca
    33961 tcagcgccag gaaatcgatg atcagttctg gcggcacgcc gtacttattc accacctctt
    34021 ccggtccgag gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct
    34081 gcgccatatc tttatcgcca gtgctgatca gcaccggacg cccggctttt tcggcttcgc
    34141 gcgccagagt accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc
    34201 ccatcgcttt aaccatcgcg tgcaagggtt cgatttgtgc acgcagatcg tccggcattg
    34261 gcgggcgatg tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt
    34321 caaagaccac cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga
    34381 ggacaccata catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt
    34441 gatatgcgcg ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct
    34501 gaaccataat gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg
    34561 aaaacatgag tttttcaccg cttttcgtga aaatcttttg tagatcttct ggatcgctcg
    34621 caaatcgtca tgtggataac tttgtgcata aattatttac atcgctttta atttattatc
    34681 atgcttttta cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc
    34741 ctaataattg agataatggg attttttcta atgtggatag atatgaatta tttttctcct
    34801 taaggatcat ccgttatttg ggtcgttttc tgctaaaatc tgcgccttgc gcttctttag
    34861 cgcactgtct atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat
    34921 aacccttttg ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat
    34981 catcattgcc gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc
    35041 gcgtttttgt gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct
    35101 gaacccacac ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta
    35161 tctgcttatt tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg
    35221 taagcacatt ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct
    35281 tggcctggcg tgctggtcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt
    35341 attacgtcat ccggagcgac gtggtaaaga tgttgaaacc actcggcgtt cttgtgaaaa
    35401 gtttcgcctg catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga
    35461 aaaacatcag caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat
    35521 tgcgatggcg ctaaatgtac tgggcaaaca attcgataaa ctgcttaatg ttacactgtg
    35581 ttatccggac aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat
    35641 tgtcgtacat gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga
    35701 caaaagcttt aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg
    35761 gcttctgacg tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga
    35821 atgagcagag gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct
    35881 tcttcgctca tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc
    35941 gaggcggtat tgttgtcaat cgtgattaac tgaaacatct gacccaatgt catcatcccg
    36001 ctgatttgtt tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta
    36061 agctgttttg cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat
    36121 ttgatatctt tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga
    36181 ttggcattta gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca
    36241 cttttttgta ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca
    36301 acttcatcgt ttgctaactc agggtgtaca gcaagctgct tctgcatagc aatgttatac
    36361 tggatgataa attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca
    36421 ccactaccga gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca
    36481 ttgatataat ctttgtcttt aacgcgatcc agcatggttt ttgcaacgaa agcatcgatt
    36541 tgggcaacgt cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca
    36601 ccaaccggaa atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc
    36661 gttactttgt tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc
    36721 aggttagaca atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaac
    36781 tttccatact ctgcaggggc aacctctaat gtcgtagaga atgagccacc aaacgcgaat
    36841 cgcaatgtcg cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt
    36901 tcattattca ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt
    36961 aacattgtga tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct
    37021 ggcttgatat tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa
    37081 ttaaaaaagc ctttgtcaaa attttgataa cttaacgtca tatcgtaggc atgttcacct
    37141 ttctttttag catcgttaaa atctttaata aatttttcga caccaggctg aatttgtgta
    37201 cctgtgtacc atgtgccacc accccagatt acggctaacg caacaataac acctgtagct
    37261 gacttacgta tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat
    37321 gctgaaataa tgtccagc
//
LOCUS       DAZFXT010000037        37040 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.037, whole genome shotgun sequence.
ACCESSION   DAZFXT010000037 DAZFXT010000000
VERSION     DAZFXT010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37040
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.037"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            550..2118
                     /gene="cydA"
                     /locus_tag="ACHKW0_001950"
     CDS             550..2118
                     /gene="cydA"
                     /locus_tag="ACHKW0_001950"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HFO7474793.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            2134..3273
                     /gene="cydB"
                     /locus_tag="ACHKW0_001951"
     CDS             2134..3273
                     /gene="cydB"
                     /locus_tag="ACHKW0_001951"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7474794.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3288..3401
                     /gene="cydX"
                     /locus_tag="ACHKW0_001952"
     CDS             3288..3401
                     /gene="cydX"
                     /locus_tag="ACHKW0_001952"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HFO7474795.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3401..3694
                     /gene="ybgE"
                     /locus_tag="ACHKW0_001953"
     CDS             3401..3694
                     /gene="ybgE"
                     /locus_tag="ACHKW0_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HFO7474796.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3844..4248
                     /gene="ybgC"
                     /locus_tag="ACHKW0_001954"
     CDS             3844..4248
                     /gene="ybgC"
                     /locus_tag="ACHKW0_001954"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7474797.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4245..4937
                     /gene="tolQ"
                     /locus_tag="ACHKW0_001955"
     CDS             4245..4937
                     /gene="tolQ"
                     /locus_tag="ACHKW0_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HFO7474798.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4941..5369
                     /gene="tolR"
                     /locus_tag="ACHKW0_001956"
     CDS             4941..5369
                     /gene="tolR"
                     /locus_tag="ACHKW0_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HFO7474799.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5434..6699
                     /gene="tolA"
                     /locus_tag="ACHKW0_001957"
     CDS             5434..6699
                     /gene="tolA"
                     /locus_tag="ACHKW0_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HFO7474800.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6832..8124
                     /gene="tolB"
                     /locus_tag="ACHKW0_001958"
     CDS             6832..8124
                     /gene="tolB"
                     /locus_tag="ACHKW0_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HFO7474801.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            8159..8680
                     /gene="pal"
                     /locus_tag="ACHKW0_001959"
     CDS             8159..8680
                     /gene="pal"
                     /locus_tag="ACHKW0_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HFO7474802.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8690..9481
                     /gene="cpoB"
                     /locus_tag="ACHKW0_001960"
     CDS             8690..9481
                     /gene="cpoB"
                     /locus_tag="ACHKW0_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HFO7474803.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9646..9721
                     /locus_tag="ACHKW0_001961"
     tRNA            9646..9721
                     /locus_tag="ACHKW0_001961"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9679..9681,aa:Lys,seq:ttt)
     gene            9857..9932
                     /locus_tag="ACHKW0_001962"
     tRNA            9857..9932
                     /locus_tag="ACHKW0_001962"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9890..9892,aa:Val,seq:tac)
     gene            9935..10010
                     /locus_tag="ACHKW0_001963"
     tRNA            9935..10010
                     /locus_tag="ACHKW0_001963"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9968..9970,aa:Lys,seq:ttt)
     gene            10160..10235
                     /locus_tag="ACHKW0_001964"
     tRNA            10160..10235
                     /locus_tag="ACHKW0_001964"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10193..10195,aa:Val,seq:tac)
     gene            10239..10314
                     /locus_tag="ACHKW0_001965"
     tRNA            10239..10314
                     /locus_tag="ACHKW0_001965"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10272..10274,aa:Lys,seq:ttt)
     gene            10461..10536
                     /locus_tag="ACHKW0_001966"
     tRNA            10461..10536
                     /locus_tag="ACHKW0_001966"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10494..10496,aa:Lys,seq:ttt)
     gene            10570..10645
                     /locus_tag="ACHKW0_001967"
     tRNA            10570..10645
                     /locus_tag="ACHKW0_001967"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10603..10605,aa:Lys,seq:ttt)
     gene            10894..11937
                     /gene="nadA"
                     /locus_tag="ACHKW0_001968"
     CDS             10894..11937
                     /gene="nadA"
                     /locus_tag="ACHKW0_001968"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HFO7474804.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11975..12694
                     /gene="pnuC"
                     /locus_tag="ACHKW0_001969"
     CDS             11975..12694
                     /gene="pnuC"
                     /locus_tag="ACHKW0_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HFO7474805.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12691..13632)
                     /gene="zitB"
                     /locus_tag="ACHKW0_001970"
     CDS             complement(12691..13632)
                     /gene="zitB"
                     /locus_tag="ACHKW0_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006829 - zinc ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HFO7474806.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13746..14126)
                     /gene="ybgS"
                     /locus_tag="ACHKW0_001971"
     CDS             complement(13746..14126)
                     /gene="ybgS"
                     /locus_tag="ACHKW0_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HFO7474807.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14442..15494
                     /gene="aroG"
                     /locus_tag="ACHKW0_001972"
     CDS             14442..15494
                     /gene="aroG"
                     /locus_tag="ACHKW0_001972"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HFO7474808.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15652..16404)
                     /gene="gpmA"
                     /locus_tag="ACHKW0_001973"
     CDS             complement(15652..16404)
                     /gene="gpmA"
                     /locus_tag="ACHKW0_001973"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HFO7474809.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16609..17649)
                     /gene="galM"
                     /locus_tag="ACHKW0_001974"
     CDS             complement(16609..17649)
                     /gene="galM"
                     /locus_tag="ACHKW0_001974"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004034 - aldose 1-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HFO7474810.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17643..18791)
                     /gene="galK"
                     /locus_tag="ACHKW0_001975"
     CDS             complement(17643..18791)
                     /gene="galK"
                     /locus_tag="ACHKW0_001975"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HFO7474811.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18795..19841)
                     /gene="galT"
                     /locus_tag="ACHKW0_001976"
     CDS             complement(18795..19841)
                     /gene="galT"
                     /locus_tag="ACHKW0_001976"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HFO7474812.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQVWANSFLPNEAEREDRLQKEYFAGQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19851..20867)
                     /gene="galE"
                     /locus_tag="ACHKW0_001977"
     CDS             complement(19851..20867)
                     /gene="galE"
                     /locus_tag="ACHKW0_001977"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO7474813.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(21128..22600)
                     /gene="modF"
                     /locus_tag="ACHKW0_001978"
     CDS             complement(21128..22600)
                     /gene="modF"
                     /locus_tag="ACHKW0_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HFO7474814.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPTNEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADVPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGDLYRYALTKIN"
     gene            complement(22668..23456)
                     /gene="modE"
                     /locus_tag="ACHKW0_001979"
     CDS             complement(22668..23456)
                     /gene="modE"
                     /locus_tag="ACHKW0_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HFO7474815.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23585..23734
                     /gene="acrZ"
                     /locus_tag="ACHKW0_001980"
     CDS             23585..23734
                     /gene="acrZ"
                     /locus_tag="ACHKW0_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HFO7474816.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23901..24674
                     /gene="modA"
                     /locus_tag="ACHKW0_001981"
     CDS             23901..24674
                     /gene="modA"
                     /locus_tag="ACHKW0_001981"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7474817.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFIIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24674..25363
                     /gene="modB"
                     /locus_tag="ACHKW0_001982"
     CDS             24674..25363
                     /gene="modB"
                     /locus_tag="ACHKW0_001982"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HFO7474818.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSILHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25366..26424
                     /gene="modC"
                     /locus_tag="ACHKW0_001983"
     CDS             25366..26424
                     /gene="modC"
                     /locus_tag="ACHKW0_001983"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HFO7474819.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26425..27243)
                     /gene="ybhA"
                     /locus_tag="ACHKW0_001984"
     CDS             complement(26425..27243)
                     /gene="ybhA"
                     /locus_tag="ACHKW0_001984"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HFO7474820.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27398..28393
                     /gene="pgl"
                     /locus_tag="ACHKW0_001985"
     CDS             27398..28393
                     /gene="pgl"
                     /locus_tag="ACHKW0_001985"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HFO7474821.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28434..29387)
                     /gene="ybhD"
                     /locus_tag="ACHKW0_001986"
     CDS             complement(28434..29387)
                     /gene="ybhD"
                     /locus_tag="ACHKW0_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7474822.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKSLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIS"
     gene            29571..30623
                     /gene="ybhH"
                     /locus_tag="ACHKW0_001987"
     CDS             29571..30623
                     /gene="ybhH"
                     /locus_tag="ACHKW0_001987"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HFO7474823.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30699..32132
                     /gene="ybhI"
                     /locus_tag="ACHKW0_001988"
     CDS             30699..32132
                     /gene="ybhI"
                     /locus_tag="ACHKW0_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HFO7474824.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            32315..34576
                     /gene="ybhJ"
                     /locus_tag="ACHKW0_001989"
     CDS             32315..34576
                     /gene="ybhJ"
                     /locus_tag="ACHKW0_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HFO7474825.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKLGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
     gene            complement(34718..36001)
                     /gene="ybhC"
                     /locus_tag="ACHKW0_001990"
     CDS             complement(34718..36001)
                     /gene="ybhC"
                     /locus_tag="ACHKW0_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030599 - pectinesterase activity
                     [Evidence IEA];
                     GO_process: GO:0042545 - cell wall modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HFO7474826.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(36136..>37040)
                     /locus_tag="ACHKW0_001991"
     CDS             complement(36136..>37040)
                     /locus_tag="ACHKW0_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7474827.1"
                     /translation="SGHKHKPLTARINSDNSVTLHSWLDRYEKILASRGIKQKTLINY
                     MSKIKAIRRGLPDAPLEDITTKEIAAMLNGYIDEGKAASAKLIRSTLSDAFREAIAEG
                     HITTNPVAATRAAKSEVRRSRLTADEYLKIYQAAESSPCWLRLAMELAVVTGQRVGDL
                     CEMKWSDIVDGYLYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIAS
                     TRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLG
                     HKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         8930 a         9021 c         9690 g         9399 t
ORIGIN      
        1 tttattagta atcctgaaac tctgcgtcgt attagccagt gaccaaaaaa agaattaagg
       61 tcaaccgtgc tgtttttgct tcgtctcttt ttatctttaa ttgccaaccg aaactaattt
      121 cagccttata actcacacat tttaaacata aatgtcacta aagttacctt attgaaacat
      181 gattaacata atttgtagga attgatattt atcaatgtat aagtcttgga aatgggcatc
      241 aaaaagagat aaattgttct cgatcaaatt ggctgaaagg cggtaattta gctataaatt
      301 gatcaccgtc gaaaaatgca aatttgcttc aacaaaaacc tgtttattgt aaggattttg
      361 cggcgtaata tatacgtggg atcaatttga gtttttatta acatgtttgc aacctttctt
      421 tacgccgttt ttgtgtgcat tcacatggta tgatgaaagt gttcaaacaa atttctattg
      481 gggcatgcgt gtgacccttt ctaacggggt tcactctcgg agtcttcatg cgatgagcaa
      541 ggagtcatga tgttagatat agtcgaactg tcgcgcttac agtttgcctt gaccgcgatg
      601 taccacttcc tttttgtgcc actgacgctc ggtatggcgt tcctgctggc cattatggaa
      661 acggtctacg tcctctccgg caaacagatt tataaagata tgaccaagtt ctggggcaag
      721 ttgtttggta tcaacttcgc tctgggtgtg gctaccggtc tgaccatgga gttccagttc
      781 gggactaact ggtcttacta ttcccactat gtaggggata tcttcggtgc gccgctggca
      841 atcgaaggtc tgatggcctt cttcctcgaa tccacctttg taggtctgtt cttcttcggt
      901 tgggatcgtc tgggtaaagt tcagcatatg tgtgtcacct ggctggtggc gctcggttca
      961 aacctgtccg cactgtggat tctggttgcg aacggctgga tgcaaaaccc aatcgcgtcc
     1021 gatttcaact ttgaaactat gcgtatggag atggtgagct tctccgagct ggtgcttaac
     1081 ccggttgctc aggtgaaatt cgttcacact gtagcgtctg gttatgtgac tggcgcgatg
     1141 ttcatcctcg gtatcagcgc atggtatatg ctgaaaggtc gtgacttcgc cttcgctaaa
     1201 cgctcctttg ctatcgctgc cagcttcggt atggctgctg ttctgtctgt tattgttctg
     1261 ggtgatgaat ccggctacga aatgggcgac gtgcagaaaa ccaaactggc tgctattgaa
     1321 gccgagtggg aaacgcaacc tgcgcctgct gcctttactc tgttcggcat tcctgatcag
     1381 gaagaggaga cgaacaaatt tgcgattcag atcccttacg cactgggcat cattgcaacg
     1441 cgttccgtgg ataccccggt tatcggcctg aaagagctga tggtgcagca tgaagaacgc
     1501 attcgtaacg ggatgaaggc gtactctctg ctcgaacaac tgcgttctgg ttctaccgac
     1561 caggcggttc gtgaccagtt caatagcatg aagaaagacc tcggttacgg tctgctgctg
     1621 aaacgctata cgccaaacgt ggctgatgcg actgaagcgc agattcaaca ggcaaccaaa
     1681 gactccatcc cgcgtgtagc gccgctgtac tttgcgttcc gtatcatggt ggcgtgtggc
     1741 ttcctgcttc tggcaatcat cgcgctctct ttctggagtg tcatccgcaa ccgcattggc
     1801 gagaaaaaat ggcttctgcg cgccgcgctg tacggtattc cgctgccgtg gattgctgta
     1861 gaagcgggct ggttcgtggc tgaatatggc cgccaaccgt gggctatcgg tgaagtgctg
     1921 ccgacagctg tggcgaactc gtcactgacc gcaggcgatc tcatcttctc aatggtgctg
     1981 atttgcggcc tgtataccct gttcctggtg gcagaattgt tcttaatgtt caaatttgca
     2041 cgcctcggcc caagcagcct gaaaaccggt cgctatcact ttgagcagtc ttccacgact
     2101 actcagccgg cacgctaaga caggagtcgt caaatgatcg attatgaagt attgcgtttt
     2161 atctggtggc tgctggttgg cgttctgctg attggttttg cagtcactga cggtttcgac
     2221 atgggggtgg gcatgctcac ccgtttcctc ggtcgtaacg acaccgagcg tcgaattatg
     2281 attaactcca ttgcaccaca ctgggacggt aaccaggttt ggctgatcac cgcgggcggc
     2341 gcactctttg ctgcctggcc gatggtctat gccgctgcgt tctccggctt ctatgtggcg
     2401 atgatcctcg tgctggcgtc tttgttcttc cgtccggtcg gttttgacta ccgctccaag
     2461 attgaagaaa cccgctggcg taacatgtgg gactggggca tcttcattgg tagcttcgtt
     2521 ccgccgctgg taattggtgt agcgttcggt aacctgttgc agggcgtacc gttcaacgtt
     2581 gatgaatatc tgcgtctgta ctacaccggt aacttcttcc agttgcttaa cccgttcggc
     2641 ctgctggcag gcgtggtgag cgtagggatg atcattactc agggcgcaac ctatctgcaa
     2701 atgcgtaccg tgggcgaact gcacctgcgt acccgtgcaa cggctcaggt ggctgcgctg
     2761 gtgacactgg tctgtttcgc actggctggc gtatgggtga tgtacggtat cgatggttat
     2821 gtcgtgaaat cgacaatgga ccattacgca gcctctaacc cactgaataa agaagtggtt
     2881 cgtgaagctg gcgcatggct ggttaacttc aacaacacgc caattctgtg ggctattccg
     2941 gcactgggtg tggttctgcc gctgctgacc atcctgactg cacgtatgga taaagccgcg
     3001 tgggcgtttg tgttctcctc cctgacgctg gcctgcatca tcctgacagc cggtatcgca
     3061 atgttcccgt ttgtgatgcc gtccagcacc atgatgaacg caagtctgac aatgtgggat
     3121 gcaacttcca gccagctgac gcttaacgtc atgacctggg ttgcggtggt tctggtaccg
     3181 atcattctgc tctacaccgc ctggtgttac tggaaaatgt tcggtcgtat caccaaagaa
     3241 gatattgaac gtaacaccca ctctctgtac taagtaagga gctaaaaatg tggtatttcg
     3301 catggattct gggaacgctt cttgcctgtt cgtttggggt aatcaccgcg ctggcgcttg
     3361 aacacgtcga atcaggcaaa gccggtcaag aagacatctg atgagtaaga ttatcgcgac
     3421 tttgtatgcg gtaatggaca agcgccccct gcgggcgctt tccttcgtga tggcgcttct
     3481 gttagcagga tgtatgtttt gggacccatc tcgttttgcc gcgaagacca gtgaactgga
     3541 aatctggcat ggtttattgc tgatgtgggc cgtctgtgct ggtgtgattc acggcgtggg
     3601 ctttcgtccg cagaaggttc tttggcaagg gattttttgc ccattgcttg ccgatattgt
     3661 tctcattgtc gggctgattt tcttcttctt ttaaatcaga attctcttta aaaaattatg
     3721 ggccgctcca ggcccataaa tttttacgct cccttaactt gccctcattc ccaaacctca
     3781 atcgcgcgcg tatagtagca gcgtttaaaa gctctaactt ttgttgcatt accgggatgt
     3841 aaagtgaata caacgctgtt tcgatggccg gttcgcgtct actatgaaga taccgatgcc
     3901 ggtggtgtgg tgtaccacgc cagttacgtc gctttttatg aaagagcacg cacagagatg
     3961 ctgcgtcatc atcacttcag tcagcaggcg ctgatggctg aacgcgttgc ctttgtggta
     4021 cgtaaaatga cggtggaata ttacgcacct gcgcggctcg acgatatgct cgaaatacag
     4081 actgaaataa catcaatgcg tggcacctct ttggttttca cgcaacgtat tgtcaacgcc
     4141 gagaatactt tgctgaatga agcagaggtt ctggttgttt gcgttgaccc actcaaaatg
     4201 aagcctcgtg cgcttcccaa gtctattgtc gcggagttta agcagtgact gacatgaata
     4261 tccttgattt gttcctgaag gctagccttc tggttaaact tatcatgttg attttgattg
     4321 gtttttcaat cgcatcttgg gccattatta tccagcggac ccgtattctt aacgcagcgg
     4381 cgcgcgaagc cgaagcgttt gaagataaat tctggtctgg aatcgaactc tctcgcctct
     4441 atcaagagag ccaggggaaa cgggataatc tgactggttc ggaacaaatc ttttacagcg
     4501 ggttcaaaga gtttgtgcgc ctgcatcgtg ccaatagcca tgcgccggaa gccgtagtgg
     4561 aaggggcgtc gcgtgctatg cgtatctcca tgaaccgtga acttgaaaat ctggaaacgc
     4621 acattccgtt cctcggtacg gttggctcca tcagcccgta tattggtctg tttggtacgg
     4681 tctgggggat catgcacgcc tttatcgccc tcggggcggt aaaacaagca acactgcaaa
     4741 tggttgcgcc cggtatcgca gaagcgttga ttgcgactgc aattggtctg ttcgccgcaa
     4801 ttccggcggt aatggcttat aaccgcctca accagcgcgt aaacaaactg gaactgaatt
     4861 acgacaactt tatggaagag tttaccgcga ttctgcaccg ccaggcgttt accgttagcg
     4921 agagcaacaa ggggtaagcc atggccagag cgcgtggacg aggtcgtcgc gatctcaagt
     4981 ccgaaatcaa cattgtaccg ttgctggacg tactgctggt gctgttgctg atctttatgg
     5041 cgacagcgcc catcatcacc cagagcgtgg aggtcgatct gccagacgct actgaatcac
     5101 aggcggtgag cagtaacgat aatccgccag tgattgttga agtgtctggt attggtcagt
     5161 acaccgtggt ggttgagaaa gatcgcctgg agcgtttacc accagagcag gtggtggcgg
     5221 aagtgtccag ccgtttcaag gccaacccga aaacggtctt tctgatcggt ggcgcaaaag
     5281 atgtgcctta cgatgaaata attaaagcac tgaacttgtt acatagtgcg ggtgtgaaat
     5341 cggttggttt aatgacgcag cctatctaaa catctgcgtt tcccttgctt gaaagagagc
     5401 gggtaacagg cgaacagttt ttggaaaccg agagtgtcaa aggcaaccga acaaaacgac
     5461 aagctcaagc gggcgataat tatttcagca gtgctgcatg tcatcttatt tgcggcgctg
     5521 atctggagtt cgttcgatga gaatatagaa gcttcagccg gaggcggcgg tggttcgtcc
     5581 atcgacgctg tcatggttga ttcaggtgcg gtagttgagc agtacaaacg catgcaaagc
     5641 caggaatcaa gcgcgaagcg ttctgatgaa cagcgcaaga tgaaggaaca gcaggctgct
     5701 gaagaactgc gtgagaaaca agcggctgaa caggaacgcc tgaagcaact tgagaaagag
     5761 cggttagcgg ctcaggagca gaaaaagcag gctgaagaag ccgcaaaaca ggccgagtta
     5821 aagcagaagc aagctgaaga ggcggcagcg aaagcggcgg cagatgctaa agcgaaggcc
     5881 gaagcagatg ctaaagctgc ggaagaagca gcgaagaaag cggctgcaga cgcaaagaaa
     5941 aaagcagaag cagaagccgc caaagccgca gccgaagcgc agaaaaaagc cgaggcagcc
     6001 gctgcggcac tgaagaagaa agcggaagcg gcagaagcag ctgcagctga agcaagaaag
     6061 aaagcggcaa ctgaagctgc tgaaaaagcc aaagcagaag ctgagaagaa agcggctgct
     6121 gaaaaggctg cagctgataa gaaagcggca gcagagaaag ctgcagccga caaaaaagca
     6181 gcagaaaaag cggctgctga aaaggcagca gctgataaga aagcagcggc agaaaaagcc
     6241 gccgcagaca aaaaagcggc agcggcaaaa gctgcagctg aaaaagccgc tgcagcaaaa
     6301 gcggccgcag aggcagatga tattttcggt gagctaagct ctggtaagaa tgcaccgaaa
     6361 acggggggag gggcgaaagg gaacaatgct tcgcctgccg ggagtggtaa tactaaaaac
     6421 aatggcgcat caggggccga tatcaataac tatgccgggc agattaaatc tgctatcgaa
     6481 agtaagttct atgacgcatc gtcctatgca ggcaaaacct gtacgctgcg cataaaactg
     6541 gcacccgatg gtatgttact ggatatcaaa cctgaaggtg gcgatcccgc actttgtcag
     6601 gctgcgttgg cagcagctaa acttgcgaag atcccgaaac caccaagcca ggcagtatat
     6661 gaagtgttca aaaacgcgcc attggacttc aaaccgtaat cgcgatgttg actgttcgga
     6721 cggtcaacat caggcaccgg ttgccacggg gttctggtag ttttgtgtat tttagtttgt
     6781 taacattctg ctaaattatc gtgggccatc ggtccagata agggagatat gatgaagcag
     6841 gcattacgag tagcatttgg ttttctcata ctgtgggcat cagttctgca tgctgaagtc
     6901 cgcattgtga tcgacagcgg tgtagattcc ggtcgtccta ttggtgttgt tcctttccag
     6961 tgggcggggc ctggtgcggc acctgaagat attggcggca tcgttgctgc tgacttgcgt
     7021 aacagcggta aatttaatcc gttagatcgc gctcgtctgc cacagcagcc gggtagtgcg
     7081 caggaagtac aaccagctgc atggtccgca ctgggcattg acgctgtagt tgtcggtcag
     7141 gtcactccga atccggatgg ttcttacaat gttgcttatc aacttgttga cactggcggc
     7201 gcaccgggta ctgtacttgc tcagaactcg tacaaagtga acaagcagtg gctgcgttat
     7261 gctggtcata ccgccagtga tgaagtgttt gaaaaactga ccggcattaa aggtgcgttc
     7321 cgtactcgta ttgcctacgt tgttcagacc aacggcggtc agttcccgta tgaactgcgc
     7381 gtatctgact atgacggtta caaccagttt gtcgttcacc gttcaccgca gccgctgatg
     7441 tctccggcgt ggtcaccaga cggttctaaa ctggcttatg tgaccttcga aagcggtcgt
     7501 tccgcgctgg ttattcagac gctggcaaat ggcgctgtac gtcaggtggc ttcattcccg
     7561 cgtcacaacg gtgcacctgc attctcgcca gacggcagca aactggcatt cgccttgtcg
     7621 aaaaccggta gtctgaacct gtacgtaatg gatttggctt ctggtcagat ccgccaggtg
     7681 actgatggtc gcagtaacaa taccgaaccg acctggttcc cggatagcca gaacctggca
     7741 tttacttctg accaggccgg tcgtccgcag gtttataaag tgaatatcaa cggcggtgcg
     7801 ccacaacgta ttacctggga aggttcgcag aaccaggatg cggatgtcag cagcgacggt
     7861 aaatttatgg taatggtcag ctccaatggt gggcagcagc acattgccaa acaagatctg
     7921 gcaacgggag gcgtacaagt tctgtcgtcc acgttcctgg atgaaacgcc aagtctggca
     7981 cctaacggca ctatggtaat ctacagctct tctcagggga tgggatccgt gctgaatttg
     8041 gtttctacag atgggcgttt caaagcgcgt cttccggcaa ctgatggaca ggtcaaattc
     8101 cctgcctggt cgccgtatct gtgataataa ttaattgaat agtaaaggaa tcattgaaat
     8161 gcaactgaac aaagtgctga aagggctgat gattgctctg cctgttatgg caattgcggc
     8221 atgttcttcc aacaagaacg ccagcaatga cggcagcgaa ggcatgctgg gtgccggcac
     8281 tggtatggat gcgaacggcg gcaacggcaa catgtcttcc gaagagcagg ctcgtctgca
     8341 aatgcaacag ctgcagcaga acaacatcgt ttacttcgat ctggacaagt acgatatccg
     8401 ttctgacttc gctcaaatgc tggatgcaca tgcaaacttc ctgcgtagca acccgtctta
     8461 caaagtcacc gtagaaggtc acgcggacga acgtggtact ccggaataca acatctccct
     8521 gggtgaacgt cgtgcgaacg ccgttaagat gtacctgcag ggtaaaggcg tttctgcaga
     8581 ccagatctcc atcgtttctt acggtaaaga aaaacctgca gtactgggtc atgacgaagc
     8641 ggcatactcc aaaaaccgtc gtgcggtact ggtttactaa gagaattgca tgagcagtaa
     8701 cttcagacat caactattga gtctgtcgtt actggttggt atagcggccc cctgggccgc
     8761 ttttgctcag gcaccaatca gtagtgtcgg ctcaggctcg gtcgaagacc gcgtcactca
     8821 acttgagcgt atttctaatg ctcacagcca gcttttaacc caactccagc aacaactttc
     8881 tgataatcaa tccgatattg attccctgcg tggtcagatt caggaaaatc agtatcaact
     8941 gaatcaggtc gtggagcggc aaaagcagat cctgttgcag atcgacagcc tcagcagcgg
     9001 tggtgcagcg gcgcaatcaa ccagcagcga tcaaagcggt gcggcggcat caacgacgcc
     9061 gacagctgat gctggtactg cgaatgctgg cgcgccggtg aaaagcggcg atgcaaacac
     9121 ggattacaat gcagctattg cgctggtgca ggataaatcc cgccaggatg acgcaatggt
     9181 ggcatttcag aatttcatca aaaattaccc tgattcaact tacctgccaa acgccaatta
     9241 ttggctgggt cagttaaact acaacaaggg taaaaaagat gatgcggcgt actattttgc
     9301 ttcggtagtg aaaaactatc cgaagtcacc aaaggctgct gatgcgatgt ttaaagtcgg
     9361 cgtcatcatg caggacaaag gtgacaccgc aaaagcgaaa gccgtatacc agcaggttat
     9421 cagtaaatac cctggtactg atggcgctaa acaggcacaa aaacgtctga acgcgatgta
     9481 atgcataaca cacgaccaga agtcgcatta tttctggtcg tgtcgtgcga atcataagca
     9541 gttgagtgat ctgcatcgaa atttttgttg cgctcaagtc tgaaatcagt aatatatgcc
     9601 gccgttgcca cgggatatca aacaaaccga aagcaacgaa aaagtgggtc gttagctcag
     9661 ttggtagagc agttgacttt taatcaattg gtcgcaggtt cgaatcctgc acgacccacc
     9721 aatcgctaag gtggaagcgg tagtaaaacg tgaaggataa cgttgcatca gcaacggccc
     9781 gaagggcgag acgaagtcga gtcatcctgc acgacccacc actaacatag ttagttgtag
     9841 tatccagcgt agtattgggt gattagctca gctgggagag cacctccatt acaaggaggg
     9901 ggtcggcggt tcgatcccgt catcacccac caccgggtcg ttagctcagt tggtagagca
     9961 gttgactttt aatcaattgg tcgcaggttc gaatcctgca cgacccacca gttttagcat
    10021 cgaagacaga tgttaagcgt gtaggataac gttgcgtcag caacggcccg tagggcgagc
    10081 gaagcgagtc atcctgcacg acccaccact aattacggtg ggttcggtgg aagtagtttg
    10141 tagtatccag cgcagtatcg ggtgattagc tcagctggga gagcacctcc cttacaagga
    10201 gggggtcggc ggttcgatcc cgtcatcacc caccactcgg gtcgttagct cagttggtag
    10261 agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc accaatttta
    10321 acatcaaact cagatgttaa gcgtgaagga taacgttgcg ccagcaacgg cccgtagggc
    10381 aagcgaagcg agtcatcctg cacgacacac caatcttaaa gattggcccc gagtagaaat
    10441 ctttcaggta acacccgtat gggtcgttag ctcagttggt agagcagttg acttttaatc
    10501 aattggtcgc aggttcgaat cctgcacgac ccaccatcct gaatgattaa agcagcataa
    10561 tcccgcaagg ggtcgttagc tcagtttgta gagcagttga cttttaatca attggtcgca
    10621 ggttcgaatc ctgcacgacc caccaatgta aaaaagcgcc ctaaaggcgc ttttttgcta
    10681 ttcaggcatc ctcaatttca ctttgtaaac ctgatgacat cgtcagagct tactgtgcaa
    10741 gcaactctac gtcggtggaa ttaggcgtaa ttcgagtgac ttttccccac cattcgacta
    10801 tcttgtttag catataaaac aaattacacc gataacagcg aatattacgc taatgtcggt
    10861 tttaacgtta agcctgtaaa acgagatggt aagatgagcg taatgtttga tccagacacg
    10921 gcgatttatc ctttcccccc gaagccgacg ccgttaagca ttgatgaaaa agcgtattac
    10981 cgcgagaaga taaaacgtct gctaaaagaa cgtaatgcgg tgatggttgc ccactactat
    11041 accgatcccg aaattcaaca actggcagaa gaaaccggtg gctgtatttc tgattctctg
    11101 gaaatggcgc gcttcggtgc aaagcatccc gcttctactt tgttagtcgc tggggtgaga
    11161 tttatgggag aaaccgccaa aattctcagt ccggaaaaaa caattctgat gccgacactt
    11221 caggctgaat gttcactgga tctcggctgc cctgttgaag aatttaacgc attttgcgat
    11281 gcccatcccg atcgtactgt cgtcgtctac gccaacactt ctgctgcggt aaaagcgcgc
    11341 gcagattggg tggtaacttc aagcattgcc gtcgaactta ttgatcatct tgatagtttg
    11401 ggtgaaaaaa tcatctgggc acccgacaaa catctggggc gttacgtgca aaaacagacg
    11461 ggtggagaca ttctatgctg gcagggtgcc tgtattgtgc atgatgaatt taagactcag
    11521 gcgttaaccc gcttgcaaga agaatacccg gatgctgcca tactggtgca tccagaatca
    11581 ccacaagcta ttgtcgatat ggcggatgcg gtcggttcca ccagtcaact gatcgctgct
    11641 gcgaaaacat tgccacatca gcggcttatc gtggcaaccg atcggggcat tttctacaaa
    11701 atgcagcagg cagtgccaga taaagagtta ctggaagcac caaccgcagg tgagggtgca
    11761 acctgccgca gctgcgcgca ttgtccgtgg atggccatga atggccttca gtccatcgca
    11821 gaggcattag aacaggaagg aagcaatcac gaggttcatg ttgatgaaag gctgcgagag
    11881 agggcgctgg tgccgctcaa tcgtatgctg gattttgcgg ctacactacg tggataacga
    11941 ataataaggc gtaacgttac gctttggggg aaagatggat ttttttagtg tgcagaatat
    12001 cctggtacat ataccaatag gggcaggcgg ttatgatctc tcatggatcg aagcggtagg
    12061 cacgatcgcc gggttgctgt gtattggcct tgccagtctg gagaagatca gcaactactt
    12121 ctttggcctg atcaacgtca ccttgtttgg cattattttc tttcagattc agctgtatgc
    12181 cagcctgcta ttacaggtgt ttttctttgc cgcgaatatt tacggttggt atgcgtggtc
    12241 gcgacaaacc agtcagaacg aggcggagtt gaaaattcgc tggttgccat tgccgaaggc
    12301 actcagctgg ttggcggttt gcgttgtttc gattggtctg atgacggtat ttatcaatcc
    12361 ggtgtttgca tttttgaccc gcgtggcagt catgatcatg caagcattag gattacaggt
    12421 tgtgatgcct gaactgcaac cggacgcttt cccgttctgg gattcatgca tgatggtgtt
    12481 atctatcgtg gcaatgattc tgatgacgcg taagtatgtg gaaaactggc tgttgtgggt
    12541 gattattaac gtgattagcg tcgttatttt tgcacttcag ggcgtttacg ccatgtctct
    12601 ggagtacatc atcctgacct ttattgcgct caacggcagc cggatgtgga tcaacagcgc
    12661 acgtgaaaga ggctcacgcg cgctgtccca ttaatggtga tgatgtgaat ggccagacac
    12721 cccttcattc aggtggcagt ctggcccatg acaaggttga tattccatct gaatggtggc
    12781 gtgctcaatc tgatagtgat ccatcaggta gtgttggatc tgatccaaca aggcatcgtg
    12841 atcgtgtggc gggatcacct gcacatgcaa cgtcatcacc ggcttctcgc ctaccatcca
    12901 tacatgtaca tggtgcacat tgcggacttc cgggatttcc cggcacatac gacgcttcag
    12961 ttcagcgata tctagcgata ccggtgcacc ttcaagtaat tcattcacgc tatctttcaa
    13021 cagtcgccac gcgctacgca gaaccagaag cgacaccagt atcgagagaa tggggtcagc
    13081 aggtgtccag ccagtccaga taataatcaa cgcggcaata atagccccaa ccgaacccag
    13141 caggtccccg agcacatgca gtgccgctgc tcgtacgttg aggtttttct cttcgctgcc
    13201 gtgatgaagt aaccagaaag aaagtatatt tgccagcaac ccagccacgg caattgccat
    13261 catcatgccg ccctcgaccg gacgcggcgt gcggaagcgt tctatcgcct cccagacaat
    13321 caaaatggta attaccacga gggctatggc attcacaaac gccgcgagcg tggtcagtct
    13381 tagccaaccg aaagtgtggc gaatggtggg aggacgacgt gaaaattgca cggcgagtag
    13441 ggcgaaaagc agggcggcag tatcggtcaa catatgaccc gcatcggcca gcaatgccag
    13501 agaaccagaa agaaaaccac caacgacttc taccagcata aacccggcag tcacgccgaa
    13561 agcatacaac aagcgacgag cattattatc ttctggcagg tgtgaggacg tgtgtgagtg
    13621 tgagtgcgcc atgacgagtc atcccttaat tattctcact taactttatg acatcatact
    13681 gcttttagaa gtgaaaaatt aaaagggaga gactccgctc tcccattatt ggctattttg
    13741 cagggttact gcgtggtacc gtcggttttg gtatcgacat cattattgat gccatcaccg
    13801 gtttgtacct ttttattgat atccggacag cgaccatctt tgcacatggt gttcttgtgc
    13861 tcttcatctt tggtcattcc gtcattgttc attgaagaac catccgaatg cagcattgtg
    13921 ccaccagaac cggtatttac cccgttattg tcgacgttat ttggcgcgac attttcacgg
    13981 gcgtcagggg ctacctggcc cgcatcagct gcggcgtttg cctggccgtt attagtttgc
    14041 gctccgctat cggcagccag tgcggcaccg ctggcaaggc ttagagtggc agtcagaaat
    14101 aatgtggcca gttttgtcat tttcatagga tgctcctgtt atggtcgtta tgtcggataa
    14161 cctcttccaa cagtgcattt gcaggtgaat ataaggcatt ggtttaagat ttcagccagg
    14221 ttatgaaacg cagcagagaa tcttgaaata attaacaaac aaaggagtta cagttagaaa
    14281 ttgtaggaga gatctcgttt ttctcgacaa tctggcgttt ttcttgctaa ttccaggatt
    14341 aatccgttca tagtgtaaaa ccccgtttac acattctgac ggaagatata gattggaagt
    14401 attgcattca ctaagataag tatggcaaca ctggaacaga catgaattat cagaacgacg
    14461 atttacgcat caaagaaatc aaagagttac ttcctcctgt cgcattgctg gaaaaattcc
    14521 ccgctactga aaatgccgcg aatacggttg cccatgcccg aaaagcgatc cataagatcc
    14581 tgaaaggtaa tgatgatcgc ctgttggttg tgattggccc atgctcaatt catgatcctg
    14641 tcgcggcaaa agagtatgcc actcgcttgc tggcgctgcg tgaagagctg aaagatgagc
    14701 tggaaatcgt aatgcgcgtc tattttgaaa agccgcgtac cacggtgggc tggaaagggc
    14761 tgattaacga tccgcatatg gataatagct tccagatcaa cgacggtctg cgtatagccc
    14821 gtaaattgct gcttgatatt aacgacagcg gtctgccagc ggcaggtgag tttctcgata
    14881 tgatcacccc acaatatctc gctgacctga tgagctgggg cgcaattggc gcacgtacca
    14941 ccgaatcgca ggtgcaccgc gaactggcat cagggctttc ttgtccggtc ggcttcaaaa
    15001 atggcaccga cggtacgatt aaagtggcta tcgatgccat taatgccgcc ggtgcgccgc
    15061 actgcttcct gtccgtaacg aaatgggggc attcggcgat tgtgaatacc agcggtaacg
    15121 gcgattgcca tatcattctg cgcggcggta aagagcctaa ctacagcgcg aagcacgttg
    15181 ctgaagtgaa agaagggctg aacaaagcag gcctgccagc acaggtgatg atcgatttca
    15241 gccatgctaa ctcgtccaaa caattcaaaa agcagatgga tgtttgtgct gacgtttgcc
    15301 agcagattgc cggtggcgaa aaggccatta ttggcgtgat ggtggaaagc catctggtgg
    15361 aaggcaatca gagcctcgag agcggggagc cgctggccta cggtaagagc atcaccgatg
    15421 cctgcatcgg ctgggaagat accgatgctc tgttacgtca actggcgaat gcagtaaaag
    15481 cgcgtcgcgg gtaaggttta attgtcggat gcgccgtcag agtggcgtat ccgatgaatc
    15541 accacaggcc tgataagtcg cgcagcgtcg catcaggcaa tgtgctccat tgttagcaac
    15601 aaaaatgccg actcacttgc agtcggcttt ctcattttaa acgaatgacg tttacttcgc
    15661 tttaccctgg tttgcaaccg ccgctgcttt cgctgcgatc tcgtcagcat tacccagata
    15721 atagcgtttc agcggtttga aattctcgtc gaactcatac accagcggca cgccagtcgg
    15781 gatattaagc tcaagaatct cttcttcgct catgttatca agatatttca ccagcgcacg
    15841 taaagagtta ccgtgtgcag cgatgatcac gcgctcaccg ctcttcatac gcggcagaat
    15901 agtttcattc cagtaaggga tcacgcggtc aatggtcagc gccaggcttt ccgtcagcgg
    15961 cagttctttt tcgctcagtt tcgcgtaacg cggatcgtga cccggataac gctcatcatc
    16021 tttagtcagt tccggcggag tcactgcaaa accacgacgc cactgtttca cctgctcgtc
    16081 gccatacttt tcagcagttt ccgctttgtt cagaccctgc aacgcaccgt agtgacgttc
    16141 gttcagtttc caggatttct caacgggcag ccatgcctga tccagttcgt ccagcacatt
    16201 ccacagggta tggatagcgc gtttcagcac agaggtgtaa gcaaagtcaa agctgtaacc
    16261 ttcctctttc agcagcttac ctgctgcttt tgcttcgctt acgcctttct cagacagatc
    16321 cacgtcgtac caaccggtga aacggttttc tttgttccac tgactttcgc catgacgaac
    16381 cagaaccagc ttagttacag ccatatactt actcctcaaa tcatttttta atgataataa
    16441 ttctcattat attgccgtga cgaagcaaca gcaatgctta cgcataacca tagcgaaaat
    16501 agtgtcgcag tgtaaggttg ttgtgaatat tgagttgcaa atatgtcggt gtttgctggt
    16561 gatttgagca atataaggat aaagccctca ttacgagggc ttcaggtgct actcagcaat
    16621 aaactgatat tccgtcaggc tggaatactc ttcgccagga cgcaggaagc agtccggttg
    16681 cggccattca gggtggtttg ggctgtccgg cagaaactcg ctttccagtg ccaggccttg
    16741 ccagtcggcg taaggttcag ttcctcttga tggcgtgccg ccgaggaagt tgccggagta
    16801 gaattgcaga gccggagcgg tggtgtagac cttcagctgc aatttttcat ctgctgacca
    16861 gacatgcgcc gccactttct tgccatcgcc tttggcctgt aacaagaatg cgtgatcgta
    16921 acctttcact ttgcgctgat cgtcgtcggc aagaaactca ctggcgatga ttttggcgct
    16981 acggaaatca aaagacgttc cggcgacaga tttcaggccg tcgtgcggaa tgccgccttc
    17041 atcaaccggc agatattcgt ccgccagaat ctgcaacttg tgattgcgca cgtcagactg
    17101 ctcgccgtca agattgaaat agacgtgatt agtcatattc accgggcaag gtttatcaac
    17161 tgtggcgcga taagtaatag agatacggtt atcgtcggtc agacgatatt gcaccgtcgc
    17221 gccgagatta cccgggaagc cctgatcgcc atcatctgaa ctcagggcaa acagcacctg
    17281 acgatcgttc tggttcacaa tctgccagcg acgtttgtcg aacccttccg ggccgccgtg
    17341 cagctggtta acgccctgac ttggcgaaag cgtcacggtt tcaccgtcaa aggtataacg
    17401 gctattggcg atgcggttgg cataacgacc aatagaggcc cccagaaacg cagcctgatc
    17461 ctgatagcat tccgggctgg cacagccgag cagcgcctcg cggacgctgc catcggaaag
    17521 cggaatacgg gcggaaagta aagtcgcacc ccagtccatc agcgtgacta ccatccctgc
    17581 gttgttacgc aaagttaaca gtcggtacgg ctgaccatcg ggtgccagtg cgggagtttc
    17641 gttcagcact gtcctgctcc ttgtgatggt ttacaaacgt aaaaagtctc tttaatacct
    17701 gtttttgctt catattgttc agcgacagct tgctgtacgg caggcaccag ttcttccggg
    17761 atcaacgcga cgatacagcc accaaatccg ccgccggtca tgcgtacgcc acctttgtca
    17821 ccaatcacag ctttgacgat ttctaccaga gtgtcaattt gcggcacggt gatttcgaaa
    17881 tcatcgcgca tagaggcatg agactccgcc attaactcgc ccatacgttt caggtcgcct
    17941 tgctccagcg cgctggcagc ttcaacggtg cgggcgtttt cggtcaggat atgacgcacg
    18001 cgtttcgcca cgatcgggtc cagttcatgc gcaacagcgt tgaactcttc aatggtgaca
    18061 tcgcgcagag ctggctgctg gaagaaacgc gcaccggttt cgcactgctc acggcgagta
    18121 ttgtactcac tgccaaccag ggtacgtttg aagttactgt tgatgatgac cacagctacg
    18181 cctttcggca tggaaactgc tttggtcccc agtgagcggc agtcaatcag caaggcatga
    18241 tctttcttgc cgagcgcgga aattagctga tccatgatcc cgcagttaca gccaacaaac
    18301 tggttttctg cttcctgacc attaagcgcg atttgtgcgc cgtccagcgg cagatgataa
    18361 agctgctgca atacggttcc gaccgcgact tccagtgaag cggaagaact taacccggcc
    18421 ccctgcggca cattgccgct gatcaccatg tccacaccgc cgaagctgtt gttacgcagt
    18481 tgcagatgtt tcaccacgcc acgaacgtag ttcgcccatt gatagttttc atgcgcgaca
    18541 atgggcgcat cgagggaaaa ctcgtcgagc tgattttcat aatcggctgc cattacgcga
    18601 actttacggt catcgcgtgg cgcacagctg atcacggttt gataatcaat cgcgcagggc
    18661 agaacgaaac cgtcgttgta gtcggtgtgt tcaccaatca aattcacgcg gccaggcgcc
    18721 tgaatggtgt gagtggcagg gtagccaaat gcgttggcaa acagagattg tgttttttct
    18781 ttcagactca tttcttacac tccggattcg cgaaaatgga tatcgctgac tgcgcgcaaa
    18841 cgctctgctg cctgttctgc ggtcaggtct cgctgggtct ctgccagcat ttcataacca
    18901 accataaatt tacgtacggt ggcggagcgc aacagaggcg gataaaagtg cgcgtgcagc
    18961 tgccagtgtt gattctcttc gccattaaat ggcgcgccgt gccagcccat agagtagggg
    19021 aaggagcact ggaagaggtt gtcataacga ctggtcagct ttttcaacgc cagtgctaaa
    19081 tcgctgcgct gggcatcggt caaatcggtg atccgtaaaa cgtgggcttt gggcagcagt
    19141 agcgtttcga acggccaggc agcccagtaa ggcactacgg ctaaccagtg ttcggtttcg
    19201 acaacggtac ggctaccgtc tgccagctcg cgctgaacat aatccaccag cattggcgat
    19261 ttctgcccgg caaaatattc tttttgcagg cggtcttcgc gctcagcttc gttaggcagg
    19321 aagctatttg cccaaacctg tccgtgcgga tgcgggttag agcagcccat cgccgcacct
    19381 ttgttttcaa agacctgcac ccacgggtac gtttttccca gctctgcggt ttgctcctgc
    19441 caggttttga cgatttccgt caatgccgca acgctcagtt ctggcaacgt tttactgtga
    19501 tccggtgaaa agcagatcac ccggctggta ccgcgcgcgc tctggcaacg catcaaagga
    19561 tcgttgcttt ctggcgcatc tggcgtgtca gacatcaagg ccgcaaaatc attagtgaaa
    19621 acgtaagtcc cggtgtaatc ggggttttta tcgcctgtca cccgcacatt acctgcgcag
    19681 aggaagcaat ctggatcgtg cgcaggtaac acctgtttgg ctggcgtttc ctgcgccccc
    19741 tgccaggggc gcttagcgcg gtgcggtgaa accagaatcc attgcccggt gagcgggttg
    19801 tagcggcgat gtggatgatc aacgggatta aattgcgtca tggtcgttcc ttaatcggga
    19861 tatccctgtg gatggcgtga ctgccagtgc caggtgtcct gcgccatttc atcgagtgtg
    19921 cgcgttacgc gccagttcag ttcgcgatcg gctttgctgg cgtccgccca gtaggccgga
    19981 aggtcgccct cgcgacgcgg tgcaaaatga tagttaaccg gtttgccgca ggctttgctg
    20041 aaggcattaa ccacgtccag cacgctgctg cctacgccag cgccgaggtt gtagatgtgt
    20101 acgcctggct tgttcgccag tttttccatc gccacgacgt gaccgtccgc cagatccatt
    20161 acgtggatgt aatcgcgtac gccagtacca tcttcggtcg gataatcgtt accaaaaatc
    20221 gccagcgagt cgcgacggcc tacagcaacc tgggcgatgt atggcatcag gttattcgga
    20281 atgccttgcg gatcttcacc catatcgccc gacggatgcg cgccaaccgg gttgaagtag
    20341 cgcagcaggg caatgctcca gtccggctgg gctttttgca ggtcggtgag gatctgttcc
    20401 accatcaatt tgcttttgcc ataagggctt tgcggtgtgc cggtcgggaa gctttcaacg
    20461 tatggaattt tgggctgatc gccataaacg gtggcggagg agctaaaaat aaagtttttg
    20521 acgttagcgg cgcgcatggc gctaatcagg cgcagagtgc cgttgacatt gttgtcgtaa
    20581 tattccagcg gtttttgtac cgattcgcca acggctttca gcccggcgaa gtggatcacg
    20641 gtgtcgatag cgtgatcgtg caggatctcg gtcatcaacg cttcgttacg aatatcgcct
    20701 tcaacaaacg ttggatgttt gccgcctaaa cgctcgataa caggcagtac gctgcgctta
    20761 ctgttacaga ggttatcaag aatgatgaca tcatgaccgt tttgcagtaa ttgcacacag
    20821 gtatgacttc caatgtaacc gctaccaccg gtaaccagaa ctctcataat tcgctccatt
    20881 aggcttatgg tatgaaataa ccatagcata acaaagatgc gaaaagtgtg acatggaata
    20941 aattagtgga atcgtttaca caagaattta gccgtttttt atgcgcgatt aagtgattat
    21001 aaaacagagg gtttatgaat gattgcgctt tttatctgaa aaaagacgcg gtttcatgcc
    21061 tgcatgcgtc gaactgtagg ccggagaggg ggcttacgcc acatccggca cagtcagcat
    21121 taccaactca gtttattttt gtcagcgcat agcgataaag gtcaccgtcc ggcacgaact
    21181 caagacgatg ggtaatacag gcaggcgcat cttcagcgtg gtgcgaaaca aacaacaatt
    21241 gcgtttcacc ttcgctaatc agcacatcaa caaaacggcg gataagttgg cgattgagcg
    21301 gatcaagccc ctgtagtggt tcatcgagaa taagcaatgt cggatgtttc accagcgcgc
    21361 ggacgatcag cgccagacgc tgctgtcccc aggaaagact atggaacgga acgtcagccg
    21421 tgcgtttatc aatgccgaga atatccagcc actgctgcac cagtttttgc tggcgatccg
    21481 aaacggcctg ataaatgcca atcgaatcaa aatagccaga aagaatcaca ttacgcacgg
    21541 tagtgctgac ccggtaatcc agatgcaaac tactgctgac gtaaccgata tgctttttga
    21601 tatcccagat ggtttcgccg ctgccgcgac gtcggccgaa aagcgtcaaa tcgttgctgt
    21661 aaccttgcgg atgatcgcca gtaatcaggc ttaataacgt cgattttccc gcaccatttg
    21721 gcccgacaat ttgccagtgc tcgccaggat tcacctgcca gctaaggtta ttaagaatgg
    21781 ggcgatcgtt ataagaaacc acgccattat tcagcacaat gcgcggttcg ttggtgggta
    21841 aggcgtgacg tgctgaaggt tcatccggct ccggcagttg cacaccttca agctgttcgc
    21901 tatgcgccag ttgcgcgacg agtgcttgct ggagcagttc ctctttagcg cccgtttccg
    21961 ctaacgtaca atccgccagc acgccagcaa actggacaaa ttccgggatc tcatcgaagc
    22021 gattgagcac cagtaccaga gtaataccgg actgatgtaa cgaggcgagt cgctcagcca
    22081 gctgctgacg tgaggcaaca tccaggccat cgaacggctc atcaagaatc aacaagtcag
    22141 gctccgacat cagcgcctga cacagcaggg tttttcgcgt ctcgccagtg gaaaggtatt
    22201 taaagcgtcg gtcgaggagg gcggtaatac cgaactgctg cgccagttgc atgcaacgcg
    22261 gtgcatcctt aacttcatcc tgaatgatct cagccgtagt gcgtccggtg tcatcttcgc
    22321 cagggccgag catatcggtg ttattccgct gccattcgtc gctgacgagc ttttgcaatt
    22381 gctcgaagga gagacgagtg atgtgggaaa actggctttg ccgttcacct ttcaaaagcg
    22441 gaagttcccc cgccagcgca cgggccagcg ccgactttcc acttccattc gaaccgacaa
    22501 acgcccaact atcacccgcg tttaacgtta gctgaggcaa ttgcagcgtt tttgtgtcgc
    22561 taagacgaaa cgtgccttgc aaaatttgca acgatgacat tttatatccc attttgtgca
    22621 gcgattactg atagggatac gtgtttcata acaaattgtc aacacgctta gcatagcgtg
    22681 gcgataatca cgctgtcggc attaaagtag gccgtgacat tctgtccttg ctgaagagaa
    22741 gtcgcttcat taaccggcac tgtggcgcac agtgtttgcc cgtcgggtag cgccattaat
    22801 acttcgcact gctctgcgcc gcgctcaata tgactaataa tacccggtaa ttggttgtcg
    22861 gcgttttgtg cgaccgcttc gtcctgagta atgcctaccc acggcgcttt tagcaatatc
    22921 aacacttctt tgccttcatc cagccccaga cgtgcgccgc tttgtgcggt aattgcgact
    22981 ttcaggcgtg tttttccgtc agccagtaag acatcaacat gctgttgaac gtcatcatga
    23041 tcgcgggcgg tgatggtgcc gaaccactgg ttacgggcgc tggtttgcag tgaaaaacgt
    23101 gagatcgcgg ccagcaggct gttcagcggc agggcgtcat cgtcacttaa cacatcaaag
    23161 gctttttgct ggatttgcgc cagtaagtca tagagctgaa tcagtcgctg accatagcgg
    23221 gtcagtactg cgccgccgcc acctttaccg cctgttgcgc gctcgaccag aatatgctca
    23281 cttaactgat tcatctcgtt aatggcatcc caggcgcttt tatagctgat accggcatct
    23341 ttcgctccct ggctaatgga accggaaagc gcaatgtgtt ttagtagcga aatgcggcgc
    23401 gggtcggcga ataatttttg ttggagctta agggtgagaa ggatttcggc ctgcataaca
    23461 atgtcctggc aaaagtctta ttgtgacgga aaacgaacgc cacgcaaagc tgaccgcaca
    23521 aaaggggagt gcttttctgt gcttagcggt tagaatagtc tcatgactat atctggagtt
    23581 gaccatgtta gagttattaa aaagtctggt attcgccgta atcatggtac ctgtcgtgat
    23641 ggccatcatc ctgggtctga tttacggtct tggtgaagta ttcaacatct tttctggtgt
    23701 tggtaaaaaa gaccagcccg gacaaaatca ttgattccct gaatgcccgc ttagtcgggc
    23761 attttctttt tctcaacttc ctgcttttcc tgccgatatt ttttcttatc tacctcacaa
    23821 aggttagtaa taactgctgg gaaaattccg agttagtcgt tatattgtcg cctacataac
    23881 gttacattaa ggggttacca atggctcgta aatggttgaa cttgtttgcc ggggcggcac
    23941 tctctttcgc tgttgctggc aatgcactgg cagatgaagg gaaaatcacg gtgttcgccg
    24001 ccgcatcact gactaacgca atgcaggaca ttgctacgca gtataaaaaa gagaaaggcg
    24061 tggatgtggt ttcttctttc gcttcgtcat ctactctcgc ccgtcagatt gaagcgggtg
    24121 cgcctgcgga tctgtttatt tctgccgatc agaaatggat ggattatgca gttgataaaa
    24181 aagcgatcga tacagctacg cgtcagacac tgctcggcaa tagcctggtc gttgtagcac
    24241 cgaaagccag cgagcagaaa gatttcatca tcgacagcaa aactaactgg acttcgctgc
    24301 tgaatggcgg tcgcctcgcg gttggcgatc cggaacatgt ccctgctggc atttatgcaa
    24361 aagaagcact gcaaaaactg ggcgcatggg atacgctctc cccgaaactg gctccggcgg
    24421 aagatgttcg tggcgcgctg gcgttggtcg aacgcaatga agcgccgttg ggcattgttt
    24481 acggttctga cgcagttgcc agcaaagggg taaaagtggt tgccaccttc ccggaagatt
    24541 cacataaaaa agtggaatat ccggttgctg ttgtggaagg gcataacaat gcgacagtga
    24601 aagcatttta tgattatctg aagggaccgc aggctgcgga aatctttaaa cgttacggat
    24661 ttacaaccaa gtaatgatac tgaccgatcc agaatggcag gcagttttat taagcctgaa
    24721 agtttcttcc ctggctgtgc tgtttagcct gccgtttggg atcttttttg cctggttact
    24781 ggtgcgttgc acgtttccgg gcaaagcttt gctcgacagc atactgcatc taccgctggt
    24841 gttaccgccc gtggtcgtcg gttacttatt attagtttcg atgggacggc gcggatttat
    24901 cggtgaacgt ctgtatgact ggtttggtat taccttcgcc tttagctggc gcggcgcggt
    24961 tctcgctgcc gccgtcatgt cgtttccgct aatggtgcgg gcaattcgtc tggcgctgga
    25021 aggggttgat gtcaaactgg aacaggccgc aagaacactg ggggccgggc gctggcgcgt
    25081 tttctttact atcacgttac cgctgacctt accgggaatt attgttggta cggtactggc
    25141 ttttgctcgt tctctcggtg aatttggcgc aaccatcacc tttgtgtcga acattcctgg
    25201 tgaaacgcga accattcctt ctgccatgta taccctgatt cagacccccg gcggggaaag
    25261 tggcgcagcg agactgtgca ttatttctat tgcgctggcg atgatctccc tgttgatttc
    25321 agaatggctg gccagaatca gccgtgaacg ggcggggcgc taatcatgct ggaactgaat
    25381 ttttcccaga ccttgggcaa ccattgcctg actattaatg aaacgctgcc cgccaatggc
    25441 atcactgcta tctttggcgt ctccggtgcc ggaaaaactt cgctgattaa cgccatcagt
    25501 ggactgacgc gcccgcaaaa agggaggatt gtcctcaatg gtcgggtact aaatgatgcc
    25561 gaaaaaggta tctgcctgac gccagaaaaa cgtcgcgttg gctatgtttt tcaggatgca
    25621 cggctgttcc cgcattacaa agtgcgtggc aatttgcgct acggcatggc gaaaagcatg
    25681 gtcgatcagt tcgataagct ggtggcgctt ttaggcattg aaccgttgct tgaccgttta
    25741 ccgggcagcc tgtccggagg cgaaaaacag cgcgtggcga ttggtcgggc tttgctgacg
    25801 gctccggaat tgctgttgct ggatgaaccg ctggcgtcac tggatattcc gcgtaaacgc
    25861 gaactgttgc cttatctgca acgtctgacg cgggaaatca acattccgat gttgtatgtc
    25921 agccattcgc tggatgagat cctccatctg gcagacagag tgatggtact ggaaaacggt
    25981 caggtgaaag cctttggcgc gctggaagaa gtctggggta gtagcgtgat gaatccgtgg
    26041 ctgccgaaag agcaacaaag tagcattctg aaagtgacgg tacttgagca tcatccgcat
    26101 tacgcgatga ctgcgctggc gctgggcgat cagcatttgt gggtcaataa gctggacgaa
    26161 ccgctgcaag ctgcgctacg tatccgcatt caggcttccg atgtttctct ggttttacaa
    26221 ccgccgcagc aaaccagcat tcgtaacgta ttgcgggcaa aagttgttaa tagttatgac
    26281 gacaacggcc aggtggaagt ggagctggaa gtcggcggta aaacgctgtg ggcgcgtatc
    26341 agcccgtggg ccagggatga actggcgatc aaacctggcc tgtggctgta cgcgcaaatt
    26401 aaaagtgtgt cgataaccgc ctgattaaat caggtggcta taaatgaact gggcaatgct
    26461 gtcggtggtg ttatcaccaa tcacaatgtt ggcgcgcgct tttaccgcgt catcggcgtt
    26521 gcccatcgcc acgcctgtac cagcggcttc cagcatactg atatcattaa agttatcgcc
    26581 gaatgccacg acgttttcca tcgaccaacc ttgcgcctca acccatttcg tcaaacgttt
    26641 acctttgctg ttgccgccgc gtgcaatatc aacctgatcg tgccaggacc attcacactc
    26701 cagtcccagt tcatgttcga catgcttacc aaaatgctgc aattgcggca ggtcatcgtg
    26761 cgtcagggcg aacttccata cggcgttaac ttgttgcgcc gtttcagcca gagaagcgac
    26821 ttgtgtgaaa gtcggacgct gttccggcgg cagggtttgc gcccagttag acgtgcgaat
    26881 gacatgcccg gtcgggtgct catagaccat tgcatcatcg acatacatca gaccgtgaat
    26941 gtggtgttca ttcagcatct caatgagttg cagggcttta ttaacgggca ttgggtccgc
    27001 ttccagcacg gtttttgcat gataatcata caaataggtg ccattacagc aaatagcagg
    27061 tgtatccagc gccagcgcct gataaaaagg atgaatagcg acgtgatggc gacctgtgac
    27121 gatgattaat tgatagcctg cttcgcgagc gcgggccagg gcttctatcg atgaaggaag
    27181 cagggtcttt ttcggggtca ataaggtgcc gtctaagtcg agagcaatca cgcgtgtggt
    27241 catggcgtat tccagattaa ggttaagaat tttcgtctgc gcgaatggta caccgatacc
    27301 actatcggac aaaattctgc gttttaattc agcattcacc gccaaaagcg actaatttta
    27361 gctgttacag tcagttgcta aatgcaaagg agcattcatg aagcaaacag tttatatcgc
    27421 cagccctgag agccagcaaa ttcacgtctg gaacctgaat catgaaggcg cactgacgct
    27481 gacacaggtt gtcgatgtgc cggggcaggt gcagccgatg gtggtcagcc cggacaaacg
    27541 ttatctctat gttggtgttc gccctgagtt tcgcgtcctg gcgtatcgta ttgccccgga
    27601 cgatggcgca ctgacctttg ccgcagagtc tgcgctgccg ggtagtccga cgcatatttc
    27661 caccgatcac caggggcagt ttgtctttgt aggttcttac aatgcgggta acgtgagcgt
    27721 aacgcgtctg gaagatggcc tgccagtggg cgtcgtcgat gtggtcgagg ggctggacgg
    27781 ttgccattcc gccaatatct caccggacaa ccgtacgctg tgggttccgg cattaaagca
    27841 ggatcgcatt tgcctgttta cggtcagcga tgatggtcat ctcgtggcgc aggaccctgc
    27901 ggaagtgacc accgttgaag gggccggccc gcgtcatatg gtattccatc caaacgaaca
    27961 atatgcgtat tgcgtcaatg agttaaacag ctcagtggat gtctgggaac tgaaagatcc
    28021 gcacggtaat atcgaatgtg tccagacgct ggatatgatg ccggaaaact tctccgacac
    28081 ccgttgggcg gctgatattc atatcacccc ggatggtcgc catttatacg cctgcgaccg
    28141 taccgccagc ctgattaccg ttttcagcgt ttcggaagat ggcagcgtgt tgagtaaaga
    28201 aggcttccag ccaacggaaa cccagccgcg cggcttcaat gttgatcaca gcggcaagta
    28261 tctgattgcc gccgggcaaa aatctcacca catctcggta tacgaaattg ttggcgagca
    28321 gggactactg catgaaaaag gccgttatgc ggtcgggcag ggaccaatgt gggtggtggt
    28381 taacgcacac taaccgctga tttaaccggc gcagtctctc ctgcgccggt gtattaactt
    28441 atctcctgta acgcatgtct ctggcgttcg acgatattgg tccacaaatt gtctttatcg
    28501 tcagtccaca aattaatcag caaggcaaaa aagcgttcag ctgccggaga aagcacggca
    28561 tctttgcggc gaataatccc caatgtccga cgtataacgg gttccaccag tgggatacca
    28621 ataatcgaag aatagggcgc atggggcatt gccagaccag gaagcgccga aatccccagt
    28681 cctgcctcca ctagtcctaa tgacgtcgaa agatgacgca cttcgtaaaa ccagtccagc
    28741 ttccagggct tgtcggctag ctgttgttct atcaacaatc ggttgccgct ggaggaacgt
    28801 acgccaatca ttttgtaacc cactaactcc tgccattcta caagctgctt tttggccaat
    28861 ggatggtcac gccgacaggc cagcacgaat ggctcgttga ccagtggggt aaaatcaatg
    28921 gatgaatttg tgacgttgtt catgtttatg ccaaagtctg attcgttaca cagcaccgat
    28981 tccatgcaat tattcgtgcc ttgttccaga atccgcactt taatattggg atacagctca
    29041 ttaaatttac cgattgccag cggtaaaaaa taaaatactg cggtcggaat acacgctaac
    29101 gtcaccatac cacgatgata agcattcata tcacgaatat taaaaagcgt ttcatcgaat
    29161 ttctttatta attcccgcgc ctccgggagc aaccttttcc ctgcttttgt taacgtaacc
    29221 ttgcgagttg tacgctcaaa tagttgaata tgtaaatctt cctccatctt ttttattctt
    29281 cgcgttaatg caggttgcgt aatattgagt gatttagcgg cattgttaaa tgaactggac
    29341 tctgccagta tgacaaatgc cttcatactt gataattcat gcttcattat tactccggaa
    29401 aatggaagcg acgattttgg gtggctggcc gttaaaaatt ttaactgcat ttagccaact
    29461 taaattaatg aaaaaatgtt attaatcgtt gagctaaagt cattagagat gctttgccct
    29521 taatgtaacc atatcgcaat aagttatgtt tttaaattga gggcattatt atgaaaaaaa
    29581 taccctgcgt gatgatgcga ggtggaacct cgaggggcgc gttcttgtta gcggaacatt
    29641 tacccgaaga tcaaacgcag cgcgataaaa tattgatggc aattatgggt tccggtaacg
    29701 atctggaaat tgacggtatt ggcggcggta atccgctgac cagtaaagtc gccattatta
    29761 gccgttccag cgatccgcgt gctgatgtcg attatctgtt tgctcaagtt attgtccatg
    29821 agcaacgtgt cgataccacg cctaattgtg gcaatatgct gtctggtgtt ggggcattcg
    29881 ccattgaaaa tggtttgatt gcagcgactt caccagttac ccgcgtacgt atccgcaacg
    29941 tcaatactgg tacgttcatc gaagctgatg tgcaaacgcc aaatggtgtt gtcgagtacg
    30001 agggtagcgc cagaattgac ggcgtaccgg gtactgccgc accggttgcg ctcactttcc
    30061 tgaatgccgc cggaaccaaa accggaaaag tcttcccgac tgataatcag attgattatt
    30121 ttgacgatgt cccggtgacc tgtatcgata tggcgatgcc agtcgtcatt attccggctg
    30181 aatatctggg aaaaacaggt tatgaattac cggcggaact ggatgccgac aaagcattat
    30241 tagcacgcat tgaatctatc cgtctacaag cgggtaaagc aatgggctta ggtgatgtca
    30301 gtaatatggt tatccctaaa cctgtgctta tttctccagc gcagaaaggc ggggcaatta
    30361 atgtacgtta ttttatgccg cattcttgcc atcgcgcgct ggcaataacc ggtgctattg
    30421 ctatttccag tagttgtgca ttggaaggca ccgtcacccg acaaatcgtc ccttctgtag
    30481 gatacggcaa tatcaatatt gaacacccca gtggtgcgct cgacgttcat ttaagtaatg
    30541 aaggtcagga tgccacgacg ttacgcgcat ctgttattcg gacgaccaga aaaatatttt
    30601 ccggtgaagt ttatcttccc tgaaaaaatt cgttgtcagg ataaggacaa tcaataaagg
    30661 acttctgtat gagtcataca gaaagaacag gattttaaat gaataagaaa tcgttatgga
    30721 agctaattct gatattagcg atcccatgta ttattggttt tatgccagct ccggcaggat
    30781 taagcgaact ggcgtgggtg ctttttggta tttacctggc ggccattgtg ggtctggtta
    30841 tcaagccttt cccggaacct gtcgtactgt taattgccgt tgctgcctca atggtggtgg
    30901 tcggtaactt atccgacggt gcgtttaaga ccaccgccgt attaagcggt tactcttcag
    30961 gtactacctg gctggtgttc tcggcgttta ccttaagcgc cgcatttgtg accaccggtt
    31021 taggtaaacg tattgcctat ctgctgattg gtaaaatcgg taacaccacg ctgggtctgg
    31081 gttacgttac ggtattcctc gatctggtac tggctccggc aacaccgtct aacaccgcgc
    31141 gtgcgggcgg cattgtgtta ccgatcatca acagcgtggc ggtggctttg gggtccgaac
    31201 cggaaaaaag tccgcgtcgt gtcggacatt acctgatgat gtccatttac atggtcacca
    31261 aaaccaccag ctatatgttc tttaccgcaa tggcggggaa cattctggcg ctgaaaatga
    31321 tcaacgacat tctgcacctg caaattagct ggggtggatg ggcgctggcc gccggattgc
    31381 cgggcatcat tatgctgctg gtcaccccgc tggtaattta caccatgtat ccaccagaaa
    31441 ttaaaaaggt ggataacaaa accatcgcta aagcgggcct tgccgaactg ggaccgatga
    31501 aaatccgcga aaaaatgctg ctcggtgtct ttgtgctggc gctactgggc tggattttca
    31561 gtaagtctct gggggttgat gaatccactg tggcaatcgt tgttatggcg accatgctgc
    31621 tgctgggtat cgttacctgg gaagacgtgg ttaaaaataa aggcggctgg aataccttaa
    31681 tctggtacgg cggtattatc ggattaagct ccttattatc gaaagttaaa ttcttcgaat
    31741 ggttagctga agtctttaaa aataacctgg catttgatgg tcacggtaac gttgctttct
    31801 tcgttattat tttcctcagc attatcgtgc gttatttctt cgcttccggt agtgcctata
    31861 tcgttgctat gttaccggta tttgccatgc tggcgaacgt ctccggcgca ccgttaatgt
    31921 taaccgcgct ggcactgttg ttctccaact cctatggcgg catggttact cactatggcg
    31981 gcgcggcagg tccggtcatc tttggcgtgg gttataacga tattaaatcc tggtggttgg
    32041 tcggtgcggt actgacgata ttaaccttcc tggtgcatat caccctcggc gtgtggtggt
    32101 ggaatatgct gatcggctgg aacatgctgt aaatattctc gtcatacttc aagttgcatg
    32161 tgctgcgtct gcgttcgctc accccagtca cttacttatg taagctcctg gggattcact
    32221 cgcttgtcgc cttcctgcaa ctcaaattat ttagagtata tccatttatt atctttctgc
    32281 gcacttcacg gtgcgcagat atctggagca tttgatgatc aagttatctg aaaaaggcgt
    32341 gtttctcgcc agtaataacg aaataattgc tgaagaacat ttcaccggcg aaattaaaaa
    32401 agaagaagcc aaaaaaggca ctattgcctg gtctattctc tcttcgcata atacgtccgg
    32461 aaatatggat aaacttaaaa ttaagtttga ttcattagcc tctcacgata ttacctttgt
    32521 tggtattgta cagaccgcta aagcgtccgg tatggaacgt ttcccgctgc cgtatgtgct
    32581 gaccaactgc cataactcac tctgcgccgt cggcggcacc attaacggtg atgaccatgt
    32641 ttttggttta tcggcagctc agcgttatgg cggtattttt gtgcctccgc atattgcggt
    32701 catccatcaa tatatgcgtg agatgatggc aggcggcggc aaaatgatcc tcgggtcaga
    32761 cagccacacc cgttacggtg cattagggac aatggcagtc ggtgagggcg gtggtgagtt
    32821 ggtaaaacag ctgcttaatg acacctggga tatcgactat ccgggagttg ttgcggtgca
    32881 tctgaccgga aaaccagcgc cgtatgtggg gccgcaggat gtggcgctgg ctatcattgg
    32941 cgcggtgttc aaaaacggtt acgtcaaaaa caaagtcatg gagttcgttg gaccgggcgt
    33001 tagcgcgctc tctaccgatt tccgtaacag cgttgacgtg atgaccacgg aaaccacctg
    33061 tttaagctcc gtctggcaaa ctgatgaaga agtgcataac tggctggcgc tgcacggtcg
    33121 cggccaggat tactgccagc ttaaccctca accgatggcg tactacgatg gctgcatcag
    33181 cgttgattta agcgccatca aaccaatgat tgcgctgccg ttccacccga gcaacgtgta
    33241 tgaaatcgac acactgaacc agaacctgac cgacattctg cgtgagattg aaattgagtc
    33301 cgaacgcgtg gcgcacggta aagccaaact ctcgctgctg gataaagtgg aaaatggtcg
    33361 cctgaaagtg cagcagggga ttatcgcggg ctgttctggc ggtaactacg aaaacgtcat
    33421 cgcggcggcg aatgcactgc gcggtcaatc ctgtggcaat gacaccttct cgctggcagt
    33481 ttacccgtca tcacagccgg tgtttatgga tctcgccaaa aaaggtgtgg tagcagattt
    33541 gattggcgca ggcgcaatca tcagaaccgc gttctgcggc ccatgctttg gcgcgggcga
    33601 tacgccaatc aacaacggat taagtattcg ccacaccacg cgtaacttcc cgaaccgcga
    33661 aggctctaag ccagctaatg ggcagatgtc agcggtggcg ttgatggacg ctcgttctat
    33721 cgctgcgact gcggcaaacg gtggctattt aacctctgcc agcgaacttg attgctggga
    33781 caacgtgccg gagtacgcct tcgatgtaac gccgtataaa aaccgtgttt atcagggctt
    33841 tgtgaaaggg gcaactcagc aaccgctgat ttacggaccg aacattaaag actggccgga
    33901 attgggtgcg ctgactgaca atatcgtcct gaaagtgtgc tcgaagatcc tcgacgaagt
    33961 gaccaccacc gacgaactga ttccttccgg tgaaacctct tcttatcgtt caaatccgat
    34021 tggtctggcg gagtttaccc tgtcacgccg cgatcccggt tatgttggca gaagtaaagc
    34081 gactgctgag ctggaaaatc agcgtctggc ggggaatgtc agcgagctga cagaggtgtt
    34141 tgcgcgcatt aagcagattg ctggtcagga gcatattgat ccgctgcaaa ctgaaattgg
    34201 cagcatggta tatgcggtga aactaggcga tggttctgcg cgtgaacagg cggcgagctg
    34261 ccagcgtgtg attggcggtc tggcgaatat tgccgaagag tacgcgacta aacgctaccg
    34321 ttctaacgtc atcaactggg ggatgttacc gctgcagatg gcggaagtgc caacctttga
    34381 agtgggggat tacatttaca tccctggcat taaagcggcg ctggataatc cgggtacgac
    34441 gtttaaaggt tatgtgatcc atgaagatgc gccggtaacg gaaattacgc tctatatgga
    34501 aagtctgact gctgaagagc gcgagattat caaggcgggt agtttgatta acttcaataa
    34561 aaaccgtcag atgtaaaaag cgccatgtga atgtaggtcg cattcggcac ttattgtcgg
    34621 atgcgatgct tgcgcatctt atccgaccta cgaatcgcat cgaatctgta ggccggataa
    34681 ggcgtttacg ccgcatccgg caaatagtta atagctctta cttcttcgcc tctgcaacca
    34741 ctttactacc cacgccgcgg ttattgtatt cccacatgcg gttgtagtta gtgtcattca
    34801 gattgcgctg tatttcgtcg ttatcatcta cgctgccggt attacccgca aacggacgat
    34861 tagagatcac cgcatcggcc cacggtttag ccgtgttaaa accttcgttg atggcgctat
    34921 cacggatcac cacctgaccg ttggtattgg catcaacatc cagcgagcgg cccagttgcg
    34981 ccacaccatc accgaaagca ttgaaacggc tgtttacggc gaggaaaccg tagtaaatgt
    35041 tggacagcgt agccggtgca aacacatacg cttcttgctg agtacgtgag ttcaccacgc
    35101 ggaattcggt gttatcgaac accactgcgc cgcgaccaga aacgatatcc acatcccctt
    35161 caatgtagct gttggtcacc agcgtacgcg gctgacggtt ggtttccaga cggttctgca
    35221 caccgctgtt ggtgacaaag aaggtgttct gacgaccgag aatgttaacg ttgttaatct
    35281 gtacctggtc accatcagta cgcagtgcca ccgccggatg gttacctgca tctacgctat
    35341 cgcccagcgt gttttcgatg gtcagatttt gcagttgcag gccattgttt tgtgaccaga
    35401 agaccgcaga gcagagaaca ccgatactgt cgctgcgttt gctctggcag ctatcgtaca
    35461 tataccacgc tggtttacct ggcatatatt tgccgcgcgg gttgacgtcg tgacgccagt
    35521 cggcagggct catgccccca tcaagggaaa gcccaatctt cacatcaatc ggtttttcac
    35581 ctgtaccgta cagagtaatt ccacccggag cagcagggac atacaccgtt ccctgatact
    35641 caccaggcat cacggcaata tactggcgct tgttagtgcg cttgataatt gccgcatcta
    35701 ccgccgcctg aatcgtggta tgcgttacac cttgagtacc cgccgggccg acaacaaagt
    35761 caggttgcgc aggcagggta atcggggaag gattccacgc tgcagcacct ggtgtcaggg
    35821 atgcaaaata gtgttgagca tcgaaattct gcgcttcttt tgccgacaga atcgggcgag
    35881 aagaggtacc aggcgcggtt tgatcagaag gacgttgatc gggtggtgtt gagctacagg
    35941 cggtcagcgt cacgccaaaa gccaatgcca gcgccagacg ggaaactgaa aatgtgttca
    36001 caggttgctc cgggctatga aatagaaaaa tgaatccgtt gaagcctgct tttttatact
    36061 aagttggcat tataaaaaaa cattgcttat caatttgttg caacgaacag gtcactatca
    36121 gtcaaaataa aatcattatt tgatttcaat tttgtcccac tccctgcctc tgtcatcacg
    36181 atactgtgat gccatggtgt ccgacttatg tccgagaaga tgttgagcaa acttatcgct
    36241 tatctgcttc tcatagagtc ttgcagacaa actgcgcaac tcgtgaaagg taggcggatc
    36301 cccttcgaag gaaagacctg atgcttttcg tgcgcgcata aaataccttg atactgtgcc
    36361 ggatgaaagc ggttcacgac gagtagatgc aattatggtt tctccgccaa gaatctcttt
    36421 gcatttatca agtgtttcct tcattgatat cccgagagca tcaacatgca atgttgtagg
    36481 gatggcaatt tttacgcctg ttttgctttg ttcgacataa agatatccat ctacgatatc
    36541 agaccacttc atttcgcata aatcaccaac tcgctgcccg gtaacaacag ccagttccat
    36601 tgcaagtctg agccaacatg gtgatgattc tgctgcttga taaattttca ggtattcgtc
    36661 agccgtaagt cttgatctcc ttacctctga ttttgctgcg cgagtggcag cgaccgggtt
    36721 tgttgttata tggccttcag ctattgcctc tcggaatgca tcgctcagtg ttgatctgat
    36781 taacttggct gacgccgcct tgccctcgtc tatgtatcca ttgagcattg ccgcaatttc
    36841 ttttgtggtg atgtcttcaa gtggagcatc aggcagcccc ctccttattg ctttaatttt
    36901 gctcatgtaa tttatgagtg tcttctgctt gattcctctg ctggcgagga ttttttcgta
    36961 gcgatcaagc catgaatgta acgtaacaga attatcactg ttgattctcg ctgtcagagg
    37021 cttgtgtttg tgtcctgaaa
//
LOCUS       DAZFXT010000038        36724 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.038, whole genome shotgun sequence.
ACCESSION   DAZFXT010000038 DAZFXT010000000
VERSION     DAZFXT010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36724)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36724)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36724
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.038"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..435)
                     /gene="slyA"
                     /locus_tag="ACHKW0_001992"
     CDS             complement(1..435)
                     /gene="slyA"
                     /locus_tag="ACHKW0_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HFO7474828.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            636..872
                     /locus_tag="ACHKW0_001993"
     CDS             636..872
                     /locus_tag="ACHKW0_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HFO7474829.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            875..1732
                     /locus_tag="ACHKW0_001994"
     CDS             875..1732
                     /locus_tag="ACHKW0_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HFO7474830.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1732..3744
                     /locus_tag="ACHKW0_001995"
     CDS             1732..3744
                     /locus_tag="ACHKW0_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7474831.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDVGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKSQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICNSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3745..4266)
                     /gene="sodC"
                     /locus_tag="ACHKW0_001996"
     CDS             complement(3745..4266)
                     /gene="sodC"
                     /locus_tag="ACHKW0_001996"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO7474832.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLTALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(4347..5243)
                     /locus_tag="ACHKW0_001997"
     CDS             complement(4347..5243)
                     /locus_tag="ACHKW0_001997"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HFO7474833.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(5292..5531)
                     /locus_tag="ACHKW0_001998"
     CDS             complement(5292..5531)
                     /locus_tag="ACHKW0_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HFO7474834.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            5634..6233
                     /gene="nemR"
                     /locus_tag="ACHKW0_001999"
     CDS             5634..6233
                     /gene="nemR"
                     /locus_tag="ACHKW0_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HFO7474835.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            6270..7367
                     /gene="nemA"
                     /locus_tag="ACHKW0_002000"
     CDS             6270..7367
                     /gene="nemA"
                     /locus_tag="ACHKW0_002000"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HFO7474836.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            7448..7855
                     /gene="gloA"
                     /locus_tag="ACHKW0_002001"
     CDS             7448..7855
                     /gene="gloA"
                     /locus_tag="ACHKW0_002001"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HFO7474837.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7958..8605
                     /gene="rnt"
                     /locus_tag="ACHKW0_002002"
     CDS             7958..8605
                     /gene="rnt"
                     /locus_tag="ACHKW0_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - RNA exonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HFO7474838.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8698..13314
                     /locus_tag="ACHKW0_002003"
     CDS             8698..13314
                     /locus_tag="ACHKW0_002003"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HFO7474839.1"
                     /translation="MADNPDSSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEQA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTIANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHQARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(13365..13712)
                     /gene="grxD"
                     /locus_tag="ACHKW0_002004"
     CDS             complement(13365..13712)
                     /gene="grxD"
                     /locus_tag="ACHKW0_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HFO7474840.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            14047..14862
                     /gene="mepH"
                     /locus_tag="ACHKW0_002005"
     CDS             14047..14862
                     /gene="mepH"
                     /locus_tag="ACHKW0_002005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HFO7474841.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTTSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14990..15571
                     /gene="sodB"
                     /locus_tag="ACHKW0_002006"
     CDS             14990..15571
                     /gene="sodB"
                     /locus_tag="ACHKW0_002006"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HFO7474842.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15717..16886)
                     /locus_tag="ACHKW0_002007"
     CDS             complement(15717..16886)
                     /locus_tag="ACHKW0_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474843.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(17052..17141)
                     /gene="cydH"
                     /locus_tag="ACHKW0_002008"
                     /gene_synonym="ynhF"
     CDS             complement(17052..17141)
                     /gene="cydH"
                     /locus_tag="ACHKW0_002008"
                     /gene_synonym="ynhF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I accessory subunit CydH"
                     /protein_id="HFO7474844.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            17440..18465
                     /gene="purR"
                     /locus_tag="ACHKW0_002009"
     CDS             17440..18465
                     /gene="purR"
                     /locus_tag="ACHKW0_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HFO7474845.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(18462..19394)
                     /gene="punR"
                     /locus_tag="ACHKW0_002010"
     CDS             complement(18462..19394)
                     /gene="punR"
                     /locus_tag="ACHKW0_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator PunR"
                     /protein_id="HFO7474846.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMNGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            19507..20718
                     /gene="punC"
                     /locus_tag="ACHKW0_002011"
     CDS             19507..20718
                     /gene="punC"
                     /locus_tag="ACHKW0_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside transporter PunC"
                     /protein_id="HFO7474847.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            21009..22157
                     /gene="cfa"
                     /locus_tag="ACHKW0_002012"
     CDS             21009..22157
                     /gene="cfa"
                     /locus_tag="ACHKW0_002012"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HFO7474848.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(22197..22838)
                     /gene="ribE"
                     /locus_tag="ACHKW0_002013"
     CDS             complement(22197..22838)
                     /gene="ribE"
                     /locus_tag="ACHKW0_002013"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004746 - riboflavin synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HFO7474849.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            23053..24426
                     /gene="mdtK"
                     /locus_tag="ACHKW0_002014"
     CDS             23053..24426
                     /gene="mdtK"
                     /locus_tag="ACHKW0_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HFO7474850.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(24467..25723)
                     /locus_tag="ACHKW0_002015"
     CDS             complement(24467..25723)
                     /locus_tag="ACHKW0_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HFO7474851.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            26031..26107
                     /locus_tag="ACHKW0_002016"
     tRNA            26031..26107
                     /locus_tag="ACHKW0_002016"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26065..26067,aa:Val,seq:gac)
     gene            26112..26188
                     /locus_tag="ACHKW0_002017"
     tRNA            26112..26188
                     /locus_tag="ACHKW0_002017"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26146..26148,aa:Val,seq:gac)
     gene            26296..26601
                     /locus_tag="ACHKW0_002018"
     CDS             26296..26601
                     /locus_tag="ACHKW0_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HFO7474852.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26727..28331
                     /locus_tag="ACHKW0_002019"
     CDS             26727..28331
                     /locus_tag="ACHKW0_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HFO7474853.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(28343..29155)
                     /gene="ydhT"
                     /locus_tag="ACHKW0_002020"
     CDS             complement(28343..29155)
                     /gene="ydhT"
                     /locus_tag="ACHKW0_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdhT"
                     /protein_id="HFO7474854.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(29159..29944)
                     /gene="phsC"
                     /locus_tag="ACHKW0_002021"
     CDS             complement(29159..29944)
                     /gene="phsC"
                     /locus_tag="ACHKW0_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HFO7474855.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSVTDYGEKIYLYCKAVRLWHWSNALLFVLLLASGLINHFALVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGGNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29941..30609)
                     /locus_tag="ACHKW0_002022"
     CDS             complement(29941..30609)
                     /locus_tag="ACHKW0_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7474856.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQQNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30673..31311)
                     /locus_tag="ACHKW0_002023"
     CDS             complement(30673..31311)
                     /locus_tag="ACHKW0_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310406.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HFO7474857.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSVAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(31324..33426)
                     /locus_tag="ACHKW0_002024"
     CDS             complement(31324..33426)
                     /locus_tag="ACHKW0_002024"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HFO7474858.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIHWDQLEAGDVNFIKDFYYLLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTHETLQR
                     LGLEDIAADLAAHNLLPA"
     gene            complement(33447..34073)
                     /locus_tag="ACHKW0_002025"
     CDS             complement(33447..34073)
                     /locus_tag="ACHKW0_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HFO7474859.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(34529..34738)
                     /gene="fumD"
                     /locus_tag="ACHKW0_002026"
     CDS             complement(34529..34738)
                     /gene="fumD"
                     /locus_tag="ACHKW0_002026"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HFO7474860.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
     gene            35093..35287
                     /locus_tag="ACHKW0_002027"
     CDS             35093..35287
                     /locus_tag="ACHKW0_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474861.1"
                     /translation="MDCRTQRLKNRHSRWAETQAHIPLHAFSMSPILRARHHHFRNTG
                     FAFRQCAQKASFSHPSQLKD"
     gene            35295..36707
                     /gene="pykF"
                     /locus_tag="ACHKW0_002028"
     CDS             35295..36707
                     /gene="pykF"
                     /locus_tag="ACHKW0_002028"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HFO7474862.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMTSRLDFNNDSRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAQKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         8916 a         9582 c         8956 g         9270 t
ORIGIN      
        1 tcaccctttg gcctgtaact caatgatatt atgctcaagt tttgcgatga gcgtaatcag
       61 ttgctccagt tcctctgcgg agatgccatg taatatttcc gcgcgggttt tgttaataac
      121 agcttccatt tcgctgatca gtggctctgc cttttccgtc agtttaatac gtttagcccg
      181 acgatcgctg gcacaagttt gacgcgaaat taaccctttt tcttccagtt ggtccagagt
      241 acggaccagt gatggctgct cgatgccaat cgcttttgcc agttgaattt gcgactggtc
      301 tggaggtaac tgatggatat tgtgtaacgt aacccaatgg gtttgtgtta actccagcgg
      361 tttcaggcga tggtctatca gagcacgcca tatgcgcacc aaccgtgcca gatcagaacc
      421 tagtggcgat tccaatttca tctccttata attagcttgc taagatatta tgcggctttt
      481 agaatagtgt gcagcaattg tattgctaaa acaaatgtat tgctgcattt ggttaccgtc
      541 agacatattt ttcagaaatt gcgcgtaaat ttttcgcact taaagaatat ttattaatct
      601 aacgcaatat attcggtcgt aaaggaatct actttgtgaa gtttatgctc aatgcaacag
      661 gattgccctt acaagatctg gtgttcggtg cgtccgtcta ttttcctccg tttttcaaag
      721 ccttcgcgtt tggattcgtc atctggcttg tcgtacaccg cctgcttcgt ggctggatct
      781 acgccggtga catctggcat cccttgttaa tggatttatc gctgtttgcg atttgcgttt
      841 gccttgctct ggcaatactg attgcgtggt aactatgtca attaaaacaa ttaagtattt
      901 ctcaacaatc attgtagcga tagttgcggt tcttgccgga tggtggctgt ggaattatta
      961 catgcaatct ccgtggacac gcgacgggaa aatacgcgca gaaaaggttt ctatcacccc
     1021 acaggtgtcg ggacgtattg ttgagctgaa tataaaagac aaccaactgg ttaacgcggg
     1081 ggatcttctg ctcacgatcg ataaaacgcc ctttcagatc gccgagctga acgcacaggc
     1141 tcagctggcg aaagcgcaat ctgacctcgc aaaagccaat aacgaagcga atcgtcgtcg
     1201 tcatctctca caaaatttta tttctgccga agaactcgat accgctaacc tcaatgttaa
     1261 agcgatgcag gccagtgtta atgccgcaca ggccacgctg aaacagacac aatggcaact
     1321 ggcgcaaacg gaaattcgcg ccccggtgag tggttgggtg actaacctca ctacccgcat
     1381 cggcgattac gctgacaccg ggaaaccgct gtttgccctt gtcgacagcc actcgtttta
     1441 cgtcattggt tattttgaag aaaccaagtt gcgccatatc cgcgaaggtg caccagcaca
     1501 aattaccctc tatagtgata acaaaacgtt acggggtcac gtttccagta tcggtcgggc
     1561 gatttatgat caaagcgttg aaagtgactc aagccttatt ccggatgtga aacctaacgt
     1621 cccctgggtt cgtctcgccc aacgcgttcc cgtccgcttt gcgctggata aagttccagg
     1681 cgatgtcacg ctggtgtcgg gcaccacctg tagcattgcc gtaggtcaat aatgaacgca
     1741 tcgtcatggt ccttgcgcaa tttgccctgg ttcaaggcca cgctggcgca atggcgttat
     1801 gcgttacgca ataccattgc catgtgtctg gcgctgacgg ttgcctatta tttgaatctg
     1861 gatgaaccct attgggcgat gacctcggct gcagtggtta gctttcccac cgttggcggt
     1921 gtcatcagca aaagcctcgg acgcatcgct ggcagtttac tgggagccat tgcggcactg
     1981 cttcttgccg ggcatacgct caatgagccg tggttttttc tattgagcat gtcggcgtgg
     2041 cttggctttt gtacctgggc ctgtgcgcac ttcacgaata acgtcgcgta tgcatttcaa
     2101 ctggcgggct acacggctgc catcatcgcc tttccgatgg ttaatattac tgaggccagc
     2161 cagctgtggg atatcgctca ggcgcgcgtt tgcgaggtga tagtcggtat tttgtgcggc
     2221 ggcatgatga tgatgatcct gcctagcagt tccgatgcta cagccctttt aaccgcattg
     2281 aaaaacatgc acgcccgatt actggaacat gccagtttac tctggcagcc tgaaacaaac
     2341 gatgccattc gtgcagcaca tgagggggtg attgggcaga tactgaccat gaatttgctg
     2401 cgtatccagg ctttctggag ccactatcgt tttcgccagc aaaacgcgcg ccttaatgcg
     2461 ctactccacc agcaattacg tatgaccagt gtcatctcca gcctgcgacg tatgttgctc
     2521 aactggccct caccgccagg tgccacgcga gaaattctcg aacaattgct gacggcgctc
     2581 gccagttcgc aaacagatgc ttacaccgtc gcacgtatta tcgccccgct acgcccgacc
     2641 aacgtcgccg actaccggca cgtcgccttc tggcagcgac tacgttattt ttgccgcctt
     2701 tatctgcaaa gtagtcagga attacatcgt ctgcaaagcg gtgtagatga tcatgccaga
     2761 ctcgcacgga catccgctct ggctcgtcat accgacaacg ccgaagctat gtggagcggg
     2821 ctgcgtacat tttgtacgtt gatgatgatt ggcgcatgga gtattgcatc gcaatgggat
     2881 gtcggtgcca atgcattaac gctggcagca attagctgcg tactctactc cgccgtcgca
     2941 gcaccgttta agtcgttgtc actgctgatg cgcacgctgg tgttactttc gctattcagc
     3001 tttgtggtca aatttggcct gatggtccag attagcgatc tgtggcaatt tttactgttt
     3061 ctctttccac tgctggcgac aatgcagctt cttaaatcgc agatgcccaa atttgccgca
     3121 ctgtgggggc aactgattgt ttttatgggt tcgtttatcg ctgtcactaa tcccccggtg
     3181 tatgattttg ctgattttct taacgataat ctggcaaaaa tcgttggcgt tgcgctggct
     3241 tggctagcgt tcgccattct gcgtccagga tcggatgctc gtaaaagccg ccgccatatt
     3301 cgcgcgctgc gccgggattt tgtcgatcag ctaagccgcc atccaacact gagtgaaagc
     3361 gaatttgaat cgctcactta tcatcacgtc agtcagttga gtaacagcca ggatgcgctg
     3421 gctcgccgtt ggttattacg ctggggtgta gtgctgctga actgttctca tgttgtctgg
     3481 caattgcgcg actgggaatc gcgttccgat ccgttatcgc gagtacggga taactgtatt
     3541 tcactgttgc ggggagtgat gagtgagcgt ggcgttcagc aaaaatcact ggcggccaca
     3601 cttgaagaat tacagcggat ttgcaacagc cttgcccgtc atcatcaacc tgccgcccgt
     3661 gagctggcgg caattgtctg gcggctgtac tgctcgcttt cgcaacttga gcaagcacca
     3721 ccgcaaggta cgctggcctc gtaattactt aattacacca caggcatagc gttcaccgcc
     3781 accgcccagc ggtttaggtt gatcggacat attatcgccg ccaacgtgga ccatcagcgc
     3841 tttgtctttg atttcatcca gtgatttcag acgaggcgcg atgacggcat cggtagcttt
     3901 gccgtcatta ttgacgacca gtgcaggcag atcgcctaaa tgcccggcac cttctggccc
     3961 ttcatgttta ccggtatttt gtggatcaag atgcccgcct gcggattccg cggcgctggc
     4021 tttgccatct ttggtggctg gctggcagct tcctttggca tgaatatgga agccatgttc
     4081 accggggggt aatgctgtca gatcgggcga aaactccaga cctttatcgg tttcagtaat
     4141 ggtgacgcta ccaattgact gccctacccc ttgcgacgtg acgaggttca tctcgacttt
     4201 ttcactggca gcttgtgcgc cggttgcaac aaccagcgcc agaatagcca gactaaaacg
     4261 tttcatagga cctccgttca ttgtgcattc ctgaataagt gtaaaccagt gacacatttt
     4321 tgaacggcgg ctattcctaa aagtgcttac ggtacgtcgt accccagtgc cgctttacgg
     4381 atacgaaacc attgttgacg ggtcattttc agtgtttctg cttcgacagc tgcccgtacg
     4441 cgctcaattt tacctgaacc gataattggc agcggctgcg atggtaaacg taatacccag
     4501 gcgtaaacca cctgttcaat cgagcccgcg tttaactcct ctgccaccac agccagttca
     4561 tcacgcagcg gctggaaata atcatcatta aacagacgac caccaccaag gcaggaccac
     4621 gccatcggac gaacacgcag ttgttgtagt tggtcgagcg tgccatccag cagtaacggc
     4681 tgatgcaccg gggatatttc cacctgatta gtggcaaggg taaacggcag acgtgattgc
     4741 aacagggcaa attgcgcagg cgtaaagttc gatacgccaa aatgacgcac tttgccgctc
     4801 tgatgcagat gtttgaacgc gtccgccact tcatcggcat ccattaacgg gtctggtcgg
     4861 tggattaaca gcagatccag atgatcggtc gcgagattaa ttagcgactg ttcggcgctc
     4921 ttaatgatgt gatcgcggtc agtgatgtaa tgaccaatga cgttttcttc acgcgcggtc
     4981 gtcgcgatac cgcatttact gacgatttcc atccgttcac gcaggtgagg tgccagtttc
     5041 agtgcctcgc caaacgccgc ttcgcactga tagccaccat aaatatcagc atggtccacg
     5101 gtggtcacgc cgagatccag atgctcttca ataaaactga ccagctggcg ggcggacata
     5161 ttccagtcca tcaatcgcca gtagcccatc acaaaacggg aaaactccgg gccttgcggc
     5221 gcaatagtaa tacgctgaac cataatcgct tcctcttatc agatatgaga ggagtatacg
     5281 caagattagg ttcaaaagag tgatggttgc tccggttcgt ctgatgacgc tggcttattt
     5341 gcgcgtaatt tgcgcattaa tcgctgccga caaaggcgca gcacctcttg tttttcgcca
     5401 tcgctcattt tattccagtt aaaacgctca tcacgactac gaaaacagcc gcgacaaaac
     5461 ccgcgttcgt cagactggca aattccccgg cacgggctct ggacgggaaa gaactctaat
     5521 tgctccgcca cttcgccctc ctcagataag attattacca ttattgaagc tgttaatgtc
     5581 caaagtagca actttgcttg cactagaccg actggtctac tacactccaa cgcatgaaca
     5641 aacacaccga acatgatact cgcgaacatc tcctggcgac gggcgagcaa ctttgcctgc
     5701 aacgtggatt caccgggatg gggctaagcg aattactaaa aaccgctgaa gtgccgaaag
     5761 ggtccttcta tcactacttt cgctctaaag aagcgtttgg cgttgccatg cttgagcgtc
     5821 attacgccgc atatcaccag cgactgactg agttgctgca atccggcgaa ggtaactacc
     5881 gcgaccgcat actggcttat taccagcaaa cactgaacca gttttgccaa catggaacca
     5941 tcagtggttg cctgacagta aaactctctg ccgaagtgtg cgatctgtca gaagatatgc
     6001 gcagcgcgat ggataaaggt gctcgcggcg tgatcgccct gctctctcag gcgctggaaa
     6061 atggccgtga gaaccattgt ttaacctttt gtggcgaacc gctacaacag gcacaagtgc
     6121 tttacgcact atggctgggt gcgaatctgc aggccaaaat ttcgcgcagt ttcgagccac
     6181 tggaaaacgc gctggcccat gtaaaaaaca ttattgcgac gcctgccgtt tagcaggcat
     6241 tttttatcac cagacgaccg ggagccttta tgtcatctga aaaactgtat tccccactga
     6301 aagtgggcgc gatcacggcg gcaaaccgta tttttatggc accgctgacg cgtctgcgca
     6361 gtattgaacc gggtgacatt cctaccccgt tgatggcgga atactatcgc caacgtgcca
     6421 gtgccggttt gattattagt gaagccacgc aaatttctgc ccaggcaaaa ggatatgcag
     6481 gtgcgcctgg catccatagt ccggagcaaa ttgccgcatg gaaaaaaatc accgctggcg
     6541 ttcatgctga aaatggtcat atggccgtgc agctgtggca caccggacgc atttctcacg
     6601 ccagcctgca acctggcggt caggcaccgg tagcgccttc agcacttagc gcgggaacac
     6661 gtacttctct gcgcgatgaa aatggtcagg cgatccgtgt tgaaacatcc atgccgcgtg
     6721 cgcttgaact ggaagagatt ccaggtatcg tcaatgattt ccgtcaggcc attgctaacg
     6781 cgcgtgaagc cggttttgat ctggtagagc tccactctgc tcacggttat ttgctgcatc
     6841 agttcctttc tccttcttca aaccatcgta ccgatcagta cggcggcagc gtggaaaatc
     6901 gcgcacgtct ggtactggaa gtggtcgatg ccgggattga agaatggggt gccgatcgca
     6961 ttggcattcg catttcgcca atcggtactt tccagaacac ggataacggc ccgaatgaag
     7021 aagccgatgc actgtatctg attgaacaac tgggtaaacg cggcattgct tatctgcata
     7081 tgtcagaacc agattgggcg gggggtgaac cgtatactga tgcgttccgc gaaaaagtac
     7141 gcgcccgttt ccacggtccg attatcggcg caggtgcata cacagtagaa aaagctgaaa
     7201 cgctgatcgg caaagggtta attgatgcgg tggcatttgg tcgtgactgg attgcgaacc
     7261 cggatctggt cgcccgcttg cagcgcaaag ctgagcttaa cccacagcgt gccgaaagtt
     7321 tctacggtgg cggcgcggaa ggctataccg attacccgac gttgtaatcc aacattgcga
     7381 gcggcgtaaa gccgccgcta tactaaaaca acattttgaa tctgttagcc attttgagga
     7441 taaaaagatg cgtcttcttc ataccatgct gcgcgttggc gatctgcaac gctccatcga
     7501 tttttatacc aaagtgctgg gcatgaaact gctgcgtacc agcgaaaacc cggaatacaa
     7561 atactcactg gcgtttgttg gctacggccc ggaaaccgaa gaagcggtga ttgaactgac
     7621 ctacaactgg ggcgtggata aatacgaact cggcactgct tatggtcaca tcgcgcttag
     7681 cgtagataac gccgctgaag cgtgcgaaaa aatccgtcaa aacgggggta acgtgacccg
     7741 tgaagcgggt ccggtaaaag gcggtactac ggttatcgcg tttgtggaag atccggacgg
     7801 ttacaaaatt gagttaatcg aagagaaaga cgccggtcgc ggtctgggca actaatctcc
     7861 tgccgggcgt gaactcatcg cgcccgcatc tttactgcat cgacaagtaa tatttgtcat
     7921 aatgcgcgct gcaatttatc cgtattaaga gaatcagatg tccgataacg ctcaacttac
     7981 cggtctgtgc gaccgttttc gtggttttta tcctgttgtg atcgatgttg aaacagccgg
     8041 atttaacgcc aaaaccgatg cgctgcttga gattgccgcc atcaccctga aaatggatga
     8101 acaaggctgg ctgatgccgg acaccacatt acatttccac gtcgaaccat ttgtcggcgc
     8161 aaatttgcaa ccagaagccc tcgccttcaa cggcattgac ccgaacgatc ccgatcgcgg
     8221 cgcggtcagc gaatacgagg cgctgcacga aatttttaaa gttgtacgta aaggtattaa
     8281 agcgagcggc tgtaaccgcg ccattatggt ggcgcacaat gccaattttg atcacagctt
     8341 tatgatggcc gccgcagaac gcgcctcact gaaacgtaac ccgttccacc ctttcgccac
     8401 ttttgacact gctgcactgg ccgggctggc actcggacaa accgtattgt caaaggcttg
     8461 ccagaccgct ggcatggact tcgacagcac ccaggcgcac tccgcgctgt acgacaccga
     8521 acgcactgct gtgctgtttt gtgaaatcgt caaccgctgg aaacgtctgg gaggctggcc
     8581 gctacctgcc gccgaagagg tgtaatcgag tcgatgcctg atgacatgca atgattcagg
     8641 catctatagt gaggctattc cacgcatcct gcatgatatt cacggggaat agcgttaatg
     8701 gcagataatc cagactcttc atcgctcctg ccggacgtgt tttcaccggc gacccgcgac
     8761 tggtttcttc gcgcctttaa acagccgacc gctgtccagt cgcaaacctg gcatgtggcg
     8821 gcgcgaagcg aacatgcgct ggtgattgca ccgaccggct ccgggaaaac gctggcagca
     8881 tttctctacg ccctcgatcg gctcttccgc gaaggcggcg aagatacccg cgaggcgcat
     8941 aagcgtaaaa cctcacgcat cctctatatt tcaccgataa aagctctggg caccgacgtt
     9001 cagcgcaact tgcagctccc gttgaagggt attgccgatg aacggcggcg gcgcggcgaa
     9061 acggaagtca atcttcgcgt agggatccgt actggcgata cgcctgcaca ggaacgcagc
     9121 aaactcaccc gtaatccgcc ggatattctg atcaccacac ccgaatcact ctatctgatg
     9181 ctgacctccc gcgcgcgcga aacgctacgc ggcgtcgaaa cggtaattat tgatgaagtc
     9241 cacgcggtag cgggcagtaa acgtggtgcg catctggcgt taagtctgga gcggctcgat
     9301 gcgctgctcc acacctcagc acagcgaatt ggcctttctg ccactgtgcg ctcagccagc
     9361 gatgtggcag catttcttgg tggcgatcgc ccggttacgg tagtcaaccc gcccgcaatg
     9421 cgccatccgc agatacgaat tgtcgtaccg gtcgccaata tggatgatgt ctcatcggtc
     9481 gccagcagca ccggcgaaga cagccatgcc ggccgggaag gctccatctg gccatatatt
     9541 gaaacgggta tccttgatga agtgttgcgc catcgctcga ccattgtctt tactaattca
     9601 cgtgggctgg cggaaaaact gacggcacga ttaaatgagc tttacgccgc acgattacag
     9661 cgttccccgt ctatcgccgt tgatgcggcc catttcgagt cgacctccgg cgcaacctct
     9721 aaccgtgtac aaagtagcga cgtttttatt gcccgctcac accacggctc cgtctctaaa
     9781 gaacaacgag caatcaccga gcaggcgctg aaatcgggtg agttacgctg cgtggtcgca
     9841 acctccagtc tcgaactggg gattgatatg ggcgcagtgg atctggtgat tcaggtggca
     9901 acgccgcttt ctgttgccag tgggttacaa cgcattggtc gcgccggaca tcaggttggc
     9961 ggtgtatcta aagggctgtt tttccctcgt acccggcgtg acttagtcga ttccgcagtc
    10021 attgtagagt gtatgttcgc aggcaggctg gaaaacctga caccgccgca taatccactc
    10081 gacgtccttg cgcagcaaac cgttgccgcc gcggcgatgg aggcattaca ggtagacgaa
    10141 tggtactccc gcgtacgccg tgccgcaccg tggaaagatc tgccaagacg tgtttttgac
    10201 gccacgctgg atatgctttc cgggcgctat ccctctggcg atttttctgc ttttcgcccc
    10261 aaactggtct ggaacaggga gaccgggata ttgaccgccc gacctggcgc tcaattgttg
    10321 gcggttacca gcggcggcac cattccggat cgtggcatgt atagcgtgtt attacccgaa
    10381 ggtgaagaac aggccggttc gcggcgggtg ggtgaactgg atgaggagat ggtatatgag
    10441 tcgcgggtga acgacattat cactctcggc gctacctcat ggcggatcca gcaaatcacc
    10501 cgcgatcagg tgattgtgac tcctgctccg ggtcgttctg cccggctccc cttctggcgt
    10561 ggtgaaggta acggacgtcc ggctgaatta ggcgagatga tcggcgattt tcttcatttg
    10621 ctggcggatg gcgcgttctt ttccgggact attcccccgt ggctggcaga agaaaatacg
    10681 atcgccaata ttcaggggtt gattgacgag caacgcaacg cgacgggcat cgttccgggg
    10741 agtcgccatc tggtcctcga acggtgccgt gatgaaattg gcgactggcg tattattttg
    10801 cactctccct atggaagacg ggtgcatgaa ccctgggcgc tggcgattgc cgggcgaata
    10861 catgccctat ggggcgctga cgcgtctgtg gtcgccagtg atgacggcat tgttgcgcgc
    10921 attcctgaca ctgatggtaa attgcccgac gccgcgattt ttttgtttga accagaaaag
    10981 ttgctgcaaa ttgtccgcga ggcggtaggc agctcggcac ttttcgccgc ccgttttcgc
    11041 gaatgcgccg cgcgggcatt attaatgccg gggcgcactc cgggccatcg caccccgctt
    11101 tggcaacaac ggctgcgcgc cagtcagttg ctggaaatcg ctcagggata tccggatttt
    11161 ccggtcattc tcgaaaccct acgcgaatgt ctgcaagatg tttatgatct tcccgcactg
    11221 gaacgtttga tgcgtcgcct gaacggtggc gaaattcaaa tatccgatgt aacgaccact
    11281 acgccctcgc ctttcgccac aagtttattg ttcggctatg tcgcggaatt tatgtaccag
    11341 agcgacgccc cgctggcaga gcgccgggca tccgtactgt cgctggacag cgagttactg
    11401 cgcaatctac tcggacaggt cgatccgggg gaattactcg acccgcaggt cattcgccag
    11461 gtggaagaag agttgcaacg actggctcct ggcagaagag cgaaaggtga agaaggattg
    11521 ttcgacctgc tgcgcgaact ggggccaatg accgttgaag acctggcgca acggcataca
    11581 ggcagcagtg aagaggttgc gtcgtatctg gaaaatcttc ttgcagtaaa acgaatcttc
    11641 ccagcgatga ttagcggaca ggagcgtctt gcctgtatgg atgatgccgc caggctgcgt
    11701 gatgccctcg gcgtacgact accagagtca ttgccagaga tttatttaca tagagtcagt
    11761 tacccgcttc gcgatctctt tctgcgctat ctccgggctc atgctctggt cacggctgaa
    11821 caactggctc atgagtttag tctcggtatt gccattgtcg aagagcagct tcagcaactg
    11881 cgtgaacagg gtctggtgat gaatctgcaa caagacatct gggtgagcga tgaagtattt
    11941 cgtcgtctgc gtttgcgctc gctgcaagcc gccagagaag cgacgcgtcc cgttgcagcc
    12001 acgacctatg cgcgattgct gctggaacgt cagggcgtat tacccgccac cgatggtagc
    12061 ccggcgctct ttgcctcaac atcgccaggc gtttatgagg gcgtagatgg cgtgatgcgg
    12121 gtgatcgaac agcttgccgg agtcggttta cccgcctcac tctgggaaag ccagatcctg
    12181 cctgcccgcg tacgcgacta ttcgtcagaa atgctcgatg aattactggc aaccggtgcg
    12241 gttatctggt cggggcaaaa aaaactgggt gaagatgacg gcctggtggc actgcatcta
    12301 caggaatatg ctgcagaatc gttcactccc gccgaagcgg atcaggcgaa tcgttcggcg
    12361 ctgcaacaag cgatagtcgc tgttctggct gacggaggag cctggtttgc acaacaaatc
    12421 agccaacgga tacgcgacaa aatcggcgaa tcggttgatc tctctgccct gcaagaggcg
    12481 ttatgggcgc tggtctggca aggcgtcatc accagcgaca tttgggcacc gttacgcgcc
    12541 ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc gcagtcacca ggctcgtcgt
    12601 ggacgtcctg tctatgcgca acccgtctcg ccgctggtat cttacaacac accaaatctg
    12661 gctggacgct ggtcgttatt gcaggtggag ccactaaacg ataccgaaag gatgctggcg
    12721 ctggcggaaa atatgctcga ccgctacggc atcatcagtc gtcaggcggt gatagccgaa
    12781 aatatccctg gcgggtttcc atcgatgcaa acgctttgtc gaagtatgga agactccggg
    12841 cgaattatgc gaggtcgttt tgtagaaggt ctgggtggcg cgcaattcgc tgaacgtctg
    12901 actattgacc gattgcgcga tctggcgaca caagccacgc aaacgcgcca ctatacacca
    12961 gtggcgctct ctgccaacga tccggctaat gtgtggggaa atcttctgcc ctggcctgca
    13021 catccggcaa cgctggttcc aacgcgtcgg gcgggtgcgc tggtggtcgt ttctggcggc
    13081 aaattgttac tctatctggc gcaaggtggc aaaaaaatgc tggtctggca ggaaaaagag
    13141 gaattactcg ccccagaggt tttccacgcg ctgactaccg cactgcgtcg cgaaccacgg
    13201 ctgcgcttta cgctaacaga agtgaatgat ctaccggtcc ggcaaacgcc gatgtttacg
    13261 ctgctgcgtg aggcgggatt ttcaagttcg ccacaagggc tggattgggg atagagaaag
    13321 gactgacgga tgcccgttcg catccgtcag tattgcagga cggattattc cgcgtccggc
    13381 tcttcagact tgtatttagc ggcagtttct ttgatcagct gctgcagttc gccacgctga
    13441 tacatttcga tcacgatatc acaaccgccg accagctcgc cgtcaaccca cagttgcggg
    13501 aaggtcggcc agttagcata tttcggcagt tccgcacgaa tgtccggatt ctgcagaata
    13561 tcaacatagg caaaacgttc gccacatgcg gcaagcgcct ggactgcctg ggcagagaaa
    13621 ccgcagctcg gcagtttcgg tgaacctttc atgtacagca ggatcgggtt ttcagcaatc
    13681 tggcgttgga ttttttcgat agtggtgctc attgtcttgc ttcctcaaac ttctttacgg
    13741 cagtaatctg acattgtagc gggtcagtgc ggcatcggaa aataacattt tcatcacgct
    13801 tttgctattt tatccctttg ctctatttgt tgcatttcaa atattcgttt cttctctgat
    13861 gcgcggcaaa ctggcgtatt tataaccatt tttagctttc actgctgcat ttttttgcgc
    13921 tcgccaacga aacgtatttt ttaacaataa aagctattaa ctttctcttc ttctatgcat
    13981 tagaatcatc aagttttgta aatcagacgc aggcatgata gacctgcctt aacagaggga
    14041 cgctcagtgg cgcggataaa ccgtatttcg atcacgctct gtgctttact ttttaccacc
    14101 ctgcctttaa cgcctatggc ccatgcttca aagcaagcca gggagagttc tgctaccact
    14161 catatcacca aaaaagcaga taaaaagaaa agcacggcaa ccaccaaaaa gacccagaaa
    14221 acagcgaaaa aagccgccag taaaagtacg accacaagca aaaccgcttc ttccgttaaa
    14281 aaatcttcca ttaccgcttc taaaaacgcc aaaactcgca gcaaacacgc cgtcaataaa
    14341 acggcctcag ccagctttac cgaaaagtgt accaaacgta agggctataa atcgcattgt
    14401 gtgaaagtca aaaatgccgc gtcaggaact cttgccgacg cgcacaaagc gaaggtgcaa
    14461 aaagctacga aagtggcaat gaataaactg atgcagcaaa ttggtaagcc atatcgttgg
    14521 ggtggcagct caccgcgtac cggttttgat tgcagcggcc tggtttatta cgcttataaa
    14581 gatttggtga aaattcgtat tccgcgtacg gcgaatgaaa tgtatcacct gcgtgatgca
    14641 gcgccaatcg aacgtagtga actgaaaaac ggcgacctgg tctttttccg tactcaggga
    14701 cgcggcacag ccgatcatgt cggcgtgtat gtcggcaacg gcaaatttat tcagtcaccg
    14761 cgcacaggtc aggaaattca aatcacttct ctcagtgaag actactggca gcgccactat
    14821 gttggcgctc gtcgggtaat gaccccaaaa acacttcgct aaaactttac cctgttgtta
    14881 cggcaacagg gtaagttcat cttttgtctc accttttaat ttgctaccct atccatacgc
    14941 acaataaggc tattgtacgt atgcaaatta ataataaagg agagtagcaa tgtcattcga
    15001 attacctgca ctaccatatg ctaaagatgc tctggcaccg cacatttctg cggaaactat
    15061 cgagtatcac tacggcaagc atcatcagac ttatgtcact aacctgaaca acctgattaa
    15121 aggtaccgcg tttgaaggta aatcactgga agagattatt cgcagctctg aaggtggcgt
    15181 attcaacaac gcagctcagg tctggaacca tactttctac tggaactgcc tggcaccgaa
    15241 cgccggtggc gaaccgactg gaaaagtcgc tgaagctatc gccgcatctt ttggcagctt
    15301 tgccgatttc aaagcgcagt ttactgatgc agcgatcaaa aactttggtt ctggctggac
    15361 ctggctggtg aaaaacagcg atggcaaact ggctatcgtt tcaacctcta acgcgggtac
    15421 tccgctgacc accgatgcga ctccgctgct gaccgttgat gtctgggaac acgcttatta
    15481 catcgactat cgcaatgcac gtcctggcta tctggagcac ttctgggcgc tggtgaactg
    15541 ggagttcgta gcgaaaaatc tcgctgcata ataactgatg gcaaatgcag cattgcctga
    15601 tgcgcttcgc ttaccatgcc aacgcggatg aggcgttaac agcgcattcg gcaagataaa
    15661 tcgcacgttg tcagcaactg taacgcagaa ggttatcctt ctgcgttttt gtttaattag
    15721 ctgttagcaa cgcaaactgt ttcaggttgt tttctggctg acataaacac cagcaataat
    15781 gccagtcccg cgacaatcgc tcccatcacc ggcacaaagc tgtatcccag cccagcggaa
    15841 attaccgcac caccagcagc tgctcccagc gcatttccaa gattaaaggc accaatattg
    15901 actgatgaag acagacccgg cgcttcactg gcgacacgca tcacgcgcat ctgtaacggc
    15961 ggtacgaccg caaaggttgc tgcgccccac accaccatgc taatagctgc gccgaactca
    16021 ttgcgggcca ggaacgggat tgccagcata atcaccatca gcagcaacaa aaagcctttc
    16081 aacgtgccgt taactgaacg atctgccagt ttgccgccga gatagttacc gatagagaat
    16141 ccgacaccaa tcagcaccag cattgccgtg acgaacactg gtgttgcgtg ggtaatactt
    16201 tgtagtaccg gagagatata ggtgtagaga gtaaacattg caccagctcc cagtaccgtc
    16261 gtcagcaatg cagacagcac ctgcggacgc attaataccg ccagctcttt tttcacttca
    16321 ggtcgtgccc ctgcaccacc tttaggtaat gagaagaaca gacttaccat tgaaatcact
    16381 cccagccccg ccgttgccag aaatgacatc cgccagccga tggtttcacc caaccaggtc
    16441 gccgccggca cgccaccgat atttgccagg gttaacccca taaacatagt ggcaactgcg
    16501 ctggcctgtt tatgttttgg caccacgctt gcggccacga ctgaacccaa accaaaaaat
    16561 gctccgtgat tcaggctggt caaaatgcgt gaaagcatca gggtcatata atccggcgcg
    16621 atggcggaaa gtacgttgcc gagcgtgaaa attgccatca ggaaaatcaa cgcactgcgg
    16681 cgggcacgat gagaaagtag aagcgtcatc agcggcgcgc caaccattac gccaactgca
    16741 taggcactga ttaacattcc ggcagcggga atcgagacat ccacaccgcg cgcaatgacg
    16801 ggcaacaagc ccattggcga gaactccgtt gtcccgatac caaacgcgcc aatcgccagc
    16861 gccagcaacg gatagttaat tttcatgcct tatctccacc tcttcgcgtc attacgcgat
    16921 attcattaaa gtggcgaaag catgacagca atcacaaaaa aatgaaaata acaaaaagag
    16981 aaaacacttt tgccattttg ctaacaaaca ggaaggagat gcgagggaga acgcgctccc
    17041 tcgagaggaa atcagtgcag cgcggcagtc aaacccacgg ccacgatcaa accgaggacg
    17101 ataatcgttg ttaccagtga aaatttaagg tcggtgctca tcaagttttc tcctttttta
    17161 ttaccacaca aatagtgata ttacgcattt ttacacactg tgatgaaaaa atctcccgtc
    17221 atttataatg ataagtgttt ttaccacttc cccttttcgt caagatcggc caaaattcca
    17281 cgcttacact atttgcgtac tggccattga ccccttcctg acgctccgtg tcgtttttcc
    17341 ggcgtaccgc aacacttttg ttgtgcgtaa ggtgtgtaaa ggcaaacgtt taccttgcga
    17401 ttttgcagga gctgaagtta gggtctggag tgaaatggaa tggcaacaat aaaagatgta
    17461 gcgaaacgag caaacgtttc cactacaact gtgtcacacg taatcaacaa aacacgtttc
    17521 gtcgctgaag aaacgcgcaa cgccgtgtgg gcagcgatta aagaattaca ctactcccct
    17581 agcgcggtgg cgcgtagcct gaaggttaac cacaccaagt ctatcggttt gctggcgacc
    17641 agcagcgaag cggcctattt tgccgagatc attgaagcgg ttgaaaaaaa ttgcttccag
    17701 aaaggttaca ccctgattct gggtaatgcg tggaacaatc ttgagaaaca gcgggcttat
    17761 ctgtcgatga tggcgcaaaa acgcgtcgat ggtctgctgg tgatgtgttc tgagtaccca
    17821 gagccgttgc tggcgatgct ggaagagtat cgccatatcc caatggtggt gatggactgg
    17881 ggtgaagcaa aagctgactt caccgatgcg gtcattgata acgcgttcga aggcggctac
    17941 atggccgggc gttatctgat tgaacgcggt caccgcgaaa tcggcgtcat ccccggcccg
    18001 ctggaacgta acaccggcgc aggccgcctt gccggtttta tgaaggcgat ggaagaagcg
    18061 atgatcaagg tgccggaaag ctggattgtt cagggtgact ttgaacccga atctggttat
    18121 cgcgccatgc agcaaatact gtcgcagccg catcgcccta ctgccgtctt ctgtggtggc
    18181 gatatcatgg caatgggcgc actttgtgct gctgatgaaa tgggtctgcg cgtcccgcag
    18241 gatgtttcgc tgatcggtta tgataacgtg cgcaacgccc gctattttac gccggcgctg
    18301 accacgatcc accagccaaa agattcgctg ggtgaaacag cgttcaacat gctgttggat
    18361 cgtatcgtca acaaacgtga agaaccgcag tccattgaag tgcatccgcg cttgattgaa
    18421 cgccgctccg tggctgacgg cccgttccgc gactatcgtc gttaatcacc cgttgcggga
    18481 gtctcttccg gctcccgcag ccactcctta ttcagcgtct cactatcgcc gagatactca
    18541 agcagccagg ttaacgcagg cgacatatca ttttgctgcc atgtcagaca acatgccgaa
    18601 tccgggaagg ggttttccaa ttcaagtgct acccacttcc cctcattaag ccacggtttg
    18661 gcgaaatgtg ttggcaccat ccctatgcat aatcctgccg agatacaggt tgccgatgat
    18721 tcccagtcag gcacgacaac tcttttttgg ttatccagca accaggtaat acgtttaggt
    18781 agcgttcgcg aggtgtcttc gcgcaccaac gacggccagt tgcgcaacgt atcatcgctg
    18841 aatgggccat tcatcaacgc cagcgggtgg tggctggcaa caacgcaact ccagcttagc
    18901 atccccatat cccggaaggc ataacgaccg cctaccggaa tcgcgcgtgt tgcgccaatc
    18961 gccagttcca cgcgcccgtc ggaaagcgca tcccagacac cgttgaacac ttcctgaaag
    19021 acaagaagtt cgacatcatc aaaatggcga taaaaatcaa cgatcatctg ccgtgtacgt
    19081 tctggcctga caatattatc cactgcgata gctaactgac cgcgccagcc gttcgctatc
    19141 tgctgacatt gctggcgggt gatctgcatt tttttgacaa cagagcgccc ttctttgaga
    19201 aaccacgctc cagcagcggt cagctccaca tcacggtgcc gtcgttcaaa gagcggcacc
    19261 gccagccact cttccagctg acgcacggta tagctgaccg cagaaggaac gcgatgcagc
    19321 tcctgtgccg cagcgctaaa actaccatta cgcgctaccg catcaacaac ttcgagtgaa
    19381 tattctgacc acatagtctg cctgcaaaat ttttgaaacc agtcatcaaa tattaccgtt
    19441 tcacaacact aatttcactc cctacacttt gcggcggtgt ttaattgaga gatttagaga
    19501 atatacatgc aacctgggaa aagattttta gtctggctgg cgggtttgag cgtactcggt
    19561 tttctggcaa ccgatatgta tctgcctgct ttcgccgcca tacaggccga cctgcaaacg
    19621 cctgcgtctg ctgtcagtgc cagccttagt ctgttccttg ccggttttgc cgcagcccag
    19681 cttctgtggg ggccactctc cgaccgttat ggtcgtaaac cggtgttatt aatcggcctg
    19741 acaatttttg cgttaggtag tctggggatg ctgtgggtag aaaacgccgc tacgctgctg
    19801 gtattgcgtt ttgtacaggc tgtgggtgtc tgcgccgcgg cggttatctg gcaagcgtta
    19861 gtgacggatt attatccttc acagaaagtt aaccgaattt ttgcgaccat catgccgctg
    19921 gtgggtctat ctccggccct ggctcctctg ttaggaagct ggctgctggt ccatttttcc
    19981 tggcaggcga ttttcgccac cctgtttgcc attaccgtgg tgctgattct gcctattttc
    20041 tggctcaaac ccacgacgaa ggcccgtaac aatagtcagg atggtctgac ctttaccgac
    20101 ctgctacgtt ctaaaaccta tcgcggcaac gtgctgatat acgcggcctg ttcagccagt
    20161 ttttttgcat ggctgaccgg ttcaccgttc atccttagtg aaatgggcta cagcccggca
    20221 gttattggtt taagttatgt cccgcaaact atcgcgtttc tgattggtgg ttatggctgt
    20281 cgcgccgcgc tgcagaaatg gcaaggcaag cagttattac cgtggttgct ggtgctgttt
    20341 gcagtcagcg tcattgcgac ctgggctgca ggcttcatta gccatgtgtc gctggtcgaa
    20401 atcctgatcc cattctgtgt gatggcgatt gccaatggcg cgatctaccc gattgttgtc
    20461 gcccaggcgc tgcgtccctt cccacacgca actggtcgcg ccgcagcgtt gcagaacact
    20521 ctacaactgg gtctgtgctt cctcgcaagt ctggtagttt cctggctgat cagtatcagc
    20581 acgccattgc tcaccaccac cagcgtgatg ttatcaacag tagtgctggt cgctctgggt
    20641 tacatgatgc aacgttgtga agaagttggc tgccagaatc atggcaatgc cgaagtcgct
    20701 catagcgaat cacactgatc tatatcgata tacttatact taggctgcta acaaaatttt
    20761 gttgtatgat tgaaattagc ggcctatact aatttcgagt tgttaaagct acgataaata
    20821 ttatgttttt acggggacag gatcgttccc gactcactat ggatggtcat ttcggcaaag
    20881 gttcctcctt tccctctgtt ctacgtcgga ttatagactc gcggtttttt ctgcgagatt
    20941 tctcacaaag cccaaaaagc gtctacgctg ttttaaggtt ctgatcaccg accagtgatg
    21001 gagaaactat gagttcatcg tgtatagaag aagtcagtgt accggatgac aactggtacc
    21061 gtatcgccaa cgaattactt agccgtgccg gtatagccat taacggttct gccccggcgg
    21121 atattcgtgt gaaaaacccc gattttttta aacgcgttct gcaagaaggc tctttggggt
    21181 taggcgaaag ttatatggat ggctggtggg aatgtgaccg actggatatg ttttttagca
    21241 aagtcttacg cgcaggtctc gagaaccaac tcccccatca tttcaaagac acgctgcgta
    21301 ttgccagcgc tcgtctcttc aatctgcaga gtaaaaaacg tgcctggata gtcggcaagg
    21361 agcattacga tttgggtaat gacttgttca gccgcatgct tgatcccttc atgcaatatt
    21421 cctgcgctta ctggaaagat gccgataatc tggaatctgc ccagcaggcg aagctcaaaa
    21481 tgatttgtga aaaattgcag ttaaaaccag ggatgcgcgt actggatatt ggctgcggct
    21541 ggggcggact ggcgcactac atggcatcta actatgacgt aagcgtggtc ggcgtcacca
    21601 tttctgccga acagcaaaaa atggctcagg aacgctgtga aggcctggat gtcaccattt
    21661 tgctgcaaga ttatcgtgac ctgaacgacc agtttgatcg tattgtttct gtggggatgt
    21721 tcgagcacgt cggaccgaaa aattacgata cctattttgc ggtggtggat cgtaatttga
    21781 aaccggaagg tatattcctg ctccatacta tcggttcgaa aaaaaccgat ctgaatgttg
    21841 atccctggat taataaatat atttttccga acggttgcct gccctctgta cgccagattg
    21901 ctcaatccag cgaaccccac ttcgtgatgg aagactggca taacttcggt gctgattacg
    21961 atactacgtt gatggcgtgg tatgaacgat tcctcgccgc atggccagaa attgcggata
    22021 actacagtga acgctttaaa cgaatgttta cctattatct gaatgcctgt gcaggtgctt
    22081 tccgcgcccg tgatattcaa ctctggcagg tcgtgttctc acgcggtgtt gaaaacggcc
    22141 ttcgagtcgc tcgctaaagg ctattctatc gccccctctc cgggggcgat ttcagatcag
    22201 gcttctgtac ctggttgatt catggcattt tctcgtgccg ccagcacacg ttctaccgta
    22261 tctaccactg cctgagtttg tggatcaatt tcaatgttga cgcgtgcgcc gagttttttc
    22321 ttcccaagag tcgtgcgttc cagagtttcc ggaattaaat ggacgcaaaa acgcgttggc
    22381 gtgacttcgc cgacggtcag gctaataccg tcgatgccaa taaatccttt gtacaggata
    22441 tatttcatca actgactatc ctggacttta aaccagatct ggcgattatt ttccgaggtt
    22501 aatattttcg ccacttcagc ggtggtcata atatgacctg acattaagtg tccgccaatt
    22561 tcatcactga atttcgccgc acgctcaacg tttacccaat cccccacttt taaatcgcca
    22621 agattggtaa tgcgtaacgt ttctttcatc aggtcaaaac tgacatggtt gccgttaatt
    22681 tccgtcacgg tcaggcagca accgttatgc gccacggaag cacctgtttc caggccgtcc
    22741 agcatgtggt cgggtaactc caccacatgc gtacgaaaat ttggtttctc gtcaatcgac
    22801 accagttttg cggtgccctg tacaatcccc gtaaacatac ttacaactcc tgaaatcagt
    22861 taagacattc tgttcagcac aatagcaggt ggcaaacgcc cttaccagtg aaggggtaag
    22921 aatgactatt ttttcactgg agaattaata aatcctcgct acaatagact gaatttcccc
    22981 tgcttcttct ttttgctgcc cattcaggcg gctttttagt ctctcatata actacaaata
    23041 aaaggtgttc acgtgcagaa gtatatcagt gaagcgcgtc tgttattagc attagcaatc
    23101 ccggtgattc tcgcgcaaat cgcccaaact gcgatgggtt ttgtcgatac cgtgatggcg
    23161 ggcggctata gtgccaccga catggcggcg gtcgctatcg gtacttctat ctggcttccg
    23221 gcgatcctct ttggtcacgg actgctgctg gcattaacgc cggttatcgc gcaattaaat
    23281 ggttccggtc gacgtgagcg cattgcgcat caggtgcgac aaggtttctg gctggcaggt
    23341 tttgtttccg ttctcattat gcttgtgctg tggaatgcag gttacattat ccgctccatg
    23401 gaaaacatcg atccggctct ggcggacaaa gccgtgggtt atctgcgtgc gttgttgtgg
    23461 ggcgcgccgg gctatctgtt cttccaggtt gcccgtaacc aatgtgaagg tctggcaaaa
    23521 accaagccgg gtatggtaat gggctttatc ggcctgctgg tgaacattcc ggtgaactat
    23581 atctttattt atggtcattt cggtatgcct gagctcggtg gcgttggttg tggcgtggct
    23641 actgcggcgg tgtattgggt catgttcctt gccatggttt cttacattaa acgcgcccgc
    23701 tccatgcgcg atattcgtaa cgaaaaaggc accgcgaaac ccgatcctgc ggttatgaaa
    23761 cgactgattc aactcggttt gccgattgcg ctggcactat tctttgaagt gacactgttt
    23821 gccgtcgtgg ctctgttagt gtctccgctc ggtattgttg atgtcgcagg acaccagatt
    23881 gccctgaact ttagttcact gatgtttgtg cttccaatgt cgctggcggc agcggtaact
    23941 atccgcgtag gttatcgtct gggtcagggc tcaacgctgg atgcgcaaac cgctgcgcgg
    24001 accgggctta tggtgggtgt ctgtatggca accctgacgg ccatattcac ggtttcactg
    24061 cgggagcaaa tcgccctgtt gtacaacgac aatcccgagg ttgtaacgct ggctgcgcat
    24121 ttgatgttgc tggcggcggt atatcagatt tctgactcaa tccaggtgat tggcagtggg
    24181 attttgcgtg gttataaaga tacgcgttcc attttctata tcacctttac ggcttactgg
    24241 gtgctgggct tgccaagcgg ctatattctg gcactgaccg atctggtcgt tgaacctatg
    24301 gggccagcag gcttctggat aggctttatt attggcctga cgtcggcagc cattatgatg
    24361 atgctgcgta tgcggttcct gcaacgtctg ccgtcagtta tcattctgca acgagcatcc
    24421 cgctaataaa gacaaggcgc aaccttcacg ggttgcgcct gtgtttttac gcaggctgga
    24481 gcgttgcgcc aatcccgtct tcgtctggct gtaatttcag agcgttgcac agagtttgcc
    24541 gactggcatc tgttatctca acgtgtatat ccccggtaac ttcccctttc acattgatga
    24601 agtcaggtga gatttctttc gcactggttt gcaggcaaat cgctccctct ccgcgcaggc
    24661 gtttcacttt cagttgcaca tgcgttgccg caccagtgat cagccacagt tttccgccat
    24721 tcagattgat gttaccaaca ataaaagtga caccgtcacc ttttaccgta ctgccgtcga
    24781 gaatcaacac accgccctgc tgtacagtaa tcgttgattg ttgcagatct gcgccatagc
    24841 caataatcat tgaagcgccg tccagtaatg caacactccc ggtcagttca accggtgcgc
    24901 gatcttcctc tacgacaaaa ttttcgtccg cgctggtggg tgacaacagg atcactgttc
    24961 caccttgtat taccgcattt tccagcgtac cggcatgcac ttcagcccga ccacccacat
    25021 tcacggacag gttttgcgtc ttaccggaac tgtagagctg aaggccatcc gtccccagac
    25081 gataaatgga tccattctca acaatagtgt cagagtcttc accaccgaga ttattcatgg
    25141 tgccaccagc aagcaacgta tcactggctt tcccggccag cataaaaacg ccgccctgat
    25201 taatctcaca attgctggct tccccacctg gatacaccaa caattcacca ccttcatcta
    25261 ccacgaccgc tgaggttgtc ccattaacca acaggccgcc ctcttgatcg agaatgattt
    25321 tttccgcgcg atgtccttcc agtacacgca ggttaccgcc attttccagc aacaaaccgc
    25381 aggcataacc ctgatcaacg ctgaattcac cttcgggatg gcggccatta accgtagcga
    25441 gcgtagagag actaattgcg cctccctcgg actgaacaat atctgtggca atggcaccgt
    25501 cgctgaccga tagcgtgcca ttgttaccgt actgtgtgca tttggcgatc ccaccggttg
    25561 ccactttcaa ccagccagcc cccgtgacca cggtattttc ggctcttccg ccagcttcaa
    25621 caatcaactc gccttcagta agttgcgtcg cgccaatgac ctcgccggtc ttaacaattt
    25681 gcacattccc gtcgctcatc aagtttttcg catcagatcc catgatttat tcctttgctg
    25741 catctgtgtg cctttattgc tacctaagtg taaaggctac ggaggattta tccacgacag
    25801 atttgagatg gtggcaaaca actctgttta aactctgata cacgaattat tgggttgtat
    25861 cagatgtaaa tgcgatcctg aataaaaatc gcccttgcaa atcaacaaaa tatagccaat
    25921 ttgcttaaaa gctctgcaaa cggtcgaaat agcgcagaaa attacgtttt gcctcttgcc
    25981 accttcccac tctgccgcta atattcgtcc ccgttgtcac ctacaacgtt gcgttcatag
    26041 ctcagttggt tagagcacca ccttgacatg gtgggggtcg ttggttcgag tccaattgaa
    26101 cgcaccatcc tgcgtccgta gctcagttgg ttagagcacc accttgacat ggtgggggtc
    26161 ggtggttcga gtccactcgg acgcaccaga ttttcttaat ctggtcttct cctttttccc
    26221 tctgtttctt ctctgtatcc aatacgttaa aagatttaca ctgtcttcgt atgcgttatc
    26281 agaaggagaa tcgctatggc aactttgtta caacttcatt ttgcttttaa tggcccattt
    26341 ggcgacgcaa tggctgagca gcttaaaccg cttgctgagt cgattaatca ggaacctggt
    26401 tttctgtgga aggtatggac agaaagtgaa aagaaccacg aagccggtgg gatctatctt
    26461 tttactgatg aaaaaagcgc ccttgcctat ctggaaaaac atactgcccg acttaaaaat
    26521 ctcggcgttg aggaagttgt cgccaaagtt ttcgatgtca atgagccact gagtcaaatc
    26581 aatcaggcaa aactcgcctg acagaattta atcaagggcg gttagcgccc ttttcatccc
    26641 tgtctgaaat ttctcaaatt ctaaaaatct caaccaaact tatctgataa cactaaattc
    26701 gaaagaatgc gtacaggtaa gtaacaatga aaaaaattgc tattgtgggt gccgggccta
    26761 cggggatcta caccttattc tcgcttctac agcaacaaac tccactttct atttctatct
    26821 tcgagcaggc tgacgaggcc ggtgtcggga tgccatacag tgatgaggaa aactcaaaaa
    26881 tgatgctggc aaatattgcc agtattgaaa taccgccgat taattgtacg tatctcgaat
    26941 ggctacaaaa gcaagaagcc agccatctcc agcgttatgg cgttaaaaaa gaaaccttgc
    27001 acgatcgtca gtttttaccg cgaattctgc tgggcgaata tttccgcgat caatttttac
    27061 gattagtaga ccaggcacga cagcaaaaat ttgcagtggc tgtttatgaa tcatgccagg
    27121 ttaccgatct gcaaattaca aatgctggcg tcatgctcgc tacaaatcag gatttaccca
    27181 gcgagacgtt tgatttagcg gtgattgcca cgggtcacgt ctggcctgat gaagaagaag
    27241 caaccagaac gtattttccc agtccgtggt caggcctgat ggaagcaaag gtcgatgcgt
    27301 gtaacgtggg tattatggga acatccttga gcggactgga tgcggcaatg gcagtggcta
    27361 ttcagcatgg ttcgttcatt gaagatgata aacaacacgt cgtttttaac cgcgataacg
    27421 caagtgaaaa gctaaatatt acgttgatgt cgcgcacggg tattttaccc gaagccgatt
    27481 tctattgccc tattccctac gagcccttac acatcgtcac cgatcaggca ttaaatgctg
    27541 agattcaaaa aggtgaagag ggccttttgg atcgggtatt tagattgata gtagaggaaa
    27601 tcaagtttgc tgatccagac tggagccaac gcatagcctt agagagcctg aatgtcgatt
    27661 cctttgctca agcctggttt gccgagcgca aacaacgcga cccatttgac tgggcagaaa
    27721 aaaatctcca ggaagtcgaa cgcaataaac gagaaaaaca tactgttccc tggcgttatg
    27781 tcattctgcg cctgcatgaa gccgtacagg aaattgttcc tcatctgaat gaacacgacc
    27841 ataaacggtt cagtaaaggc cttgccaggg ttttcatcga taattatgcg gcaatccctt
    27901 cagagtctat tcgtcgccta cttgccttac gtgaagcggg aatcattcat attctcgccc
    27961 tcggtgaaga ctacaaaatg gaaattaatg agtcgcgcac cgtcctgaaa acggaagaca
    28021 acagctactc gtttgacgtt tttattgatg cccgcggaca gcgtccgctt aaagtgaaag
    28081 atattccttt ccctgggcta cgcgagcaat tacagaaaac aggggatgaa atccctgatg
    28141 ttggcgaaga ttatacgtta cagcaacccg aagatattcg tggacgcgta gcgttcggcg
    28201 cgttgccctg gttgatgcac gaccagcctt tcgttcaggg acttacggca tgtgcagaaa
    28261 ttggtgaggc gatggctcgg gcggtcgtaa agcctgcatc ccgtgctcgt cggcgtcttt
    28321 cgtttgatta aaggtaaagc tatcagcact caacaaactg atgctgttca ttaagacgat
    28381 agcggactcc ggttcgaata ttgttttcac cttcaatggc gacggcgaag cgaactcttt
    28441 cgccgtcatg atatgccagt gcggcgcttc cccctggccc gagaataatt gagtcgacca
    28501 cgcccgtgct cactaccgtt gaattttcac cgctggcaat gatgtgtaca ttgtcgccgc
    28561 tgttggcaat tctggcgttt gcgccaaagc tggcgatttg caccaggtca ccattactgg
    28621 cgacatgaca ccgttcaccc aatgtacaaa cgcgtactcg cattccggta ttcgcaatcc
    28681 gactactatc tccagcgctg cttatccgcg aagaattacc ggttacagca acccgtgcgc
    28741 gttcaccgga gctgccaatg tgactgttaa agccgacact tccaatccgc acgctataac
    28801 ccatactggc aatttgcgcg gcatacccag cggttgcgat tcgggcgtta tcctcacaac
    28861 agccgatgcg agcatgatcg tcgctgcggg caaactgact gcaaaatggt atctctccgg
    28921 ggtctgtggc cagtttttgc atagaggaaa cttcctgatg cgcgaagttt tcatccgcca
    28981 gccatttttt ccacgcatac tcaaccagac tctccgccca gtccgtatat ccttcctcaa
    29041 tcagtgcatg atgaatatcg gcataactac cgccgcgtgg gaaatggcgc aggaaccagc
    29101 gatacatcac cgctccgata cgccattctc gcaggtctgc tcgtgtaatg atcattcgtt
    29161 agtgccggtg atagccatcg accatgcttt taaaggtttc gtgtggcgta cgccccgtgg
    29221 tgcaaagata aagatgaccg aagataaaaa agaggcttat aaatgccaga gcaaaatgcg
    29281 cctgcaataa ccagtatctt acgccaggaa acacatctcc cacggcttgc ggatagagac
    29341 acagcagccc cgtcaatagt aacaacggca gcaatccata cataacacca acataggcga
    29401 cctgctgtaa gggattaaat ttagactggg ttgttgccgg gaaaggatgt tcttccccct
    29461 gcataatgcc aaacaaataa aatcgcgttt gttttgccgc tcgctccagc cacccctgac
    29521 gacgaatgcg atagtggtga ccattacccc caacggcatt gatcagcaca aagccgagcc
    29581 agcatgcgag taacaaaaat ccgcaaactt catgcaccgc aaccagactt ttaaccgcag
    29641 ttgcgcccac cagcgcaaag tgatttatca gcccacttgc cagcaacaat acaaagagta
    29701 acgcattcga ccagtgccat aatctgaccg ctttgcagta aagataaatc ttctcaccat
    29761 aatcggtgac tgatttcttc acgccacgag cacgaagcaa ggcatgcagc cccagcacca
    29821 accacatccc aaccagcaat actccggcaa tgatcaacca caccggccaa aattcgggag
    29881 tgaactgtgg cacatagttc gctaactggc tctgaaactg ttcagcatgt tgcgacgggt
    29941 tcatacattt tcctttttaa tcaaatgttg accgaaccgg cgatacagat gcggtttccc
    30001 ggcaccaggt agctgatatt gatagtattt attttgctgc aaccacgcct gaatttctgg
    30061 gctatcttcg cgaccaaaga tcaatgcgtg ttccgggcag gcgctgacgc aaatgggcgg
    30121 aaagcctttt gccagtcggg actcagcgca gaaatcacat ttatctgcca ctttagtgac
    30181 agggttaagg tagcgaacct ggtacggaca agcgccaatg caataactgc agccgatgca
    30241 ctgcgatttt tctacccgca cgatcccctg ctcatcacgc cacgacgccc ctgtcgggca
    30301 tacgtcgatg cacggagcat cttcgcagtg ctgacatgac tggcgaaaaa agtgatactg
    30361 cgtttcgtta tcgttatcgg tgactggaat gtgtgctatc gataaacggc ttccctgagc
    30421 ggggacgtgg ttagttttac ggcatgcgcg ggcgcaaata ttacagccat tgcaccgtga
    30481 ctcgtcatga atcatggcat accgaacttc cttttcttgc ctcgtgttcg ctaacagaga
    30541 agacgcagaa ccagtaaaaa agattactgt tcccatcccc agaacaaatt tgcgtcgagt
    30601 gaaggacatc tgattatccc ctaatgcagt ggctgcaact gtccacccta tccatgacat
    30661 ctccctgaaa tttcaggggt tctgaaactg ctctacctcg aaccattcgc ataagccata
    30721 agccttgcca taggtcataa atcgctcgct aaacaagtac aacaccgccg tcgatgactc
    30781 cacctgccgt atatcggcat attcaggtgc aacatccatc gcggcgatcg cggcttcaat
    30841 ctgcgcttcc tggaagtaat atggcgcttg catcagcatg gcgactttgt aaggacgagg
    30901 atacgtctgg cactcaaaac gaacggcgtg tgcaatggcg cgacaaatgt cctgctccac
    30961 aacttgcaaa gacatcgccg cgtagttttc actcatggcc tgcgttgagt aatagtaatc
    31021 atcctgactt cccgtcatac aagcgatatc cgcgtagtct ccgttctgct taatgctttc
    31081 cagcaaaact gacagttcct cttctgcaac cgaatagggt ggcgagagaa acacggcgcg
    31141 cgccaccagc tgcccagcca cagaatgctg gcgaatgaac tctgccagga tttcagccgg
    31201 ttctggttcg gttaccgtat caacggggtg acgcatcccc tgtaaccatt gccgcttcgc
    31261 ctcgtcaact tcgctgactt tcgctaacgg caattcatcc cgatggttca tctcacccat
    31321 tccttacgca ggcagtagat tgtgtgctgc cagatcggcg gcaatatctt ccagccccag
    31381 acgctgcaat gtttcgtggg tcggacaacc aagctgtggg tcccagccca tttctttgta
    31441 gaacatcgtg agcgaagcat gcatatcgtc acgatccatt ttgtcagtac cttcagtaaa
    31501 gaccgggatc tgcggatcct tgtcgaatac ccaggaacag ataagatcgt gttcattacg
    31561 catatctttg gtttgcatca gttttaccgt gtaggcacga tgcaacgtaa agatacgctc
    31621 tgcagctaaa tctaattttt cctgagtcat ctcttcgccg gtgatcgctt tgaagaattt
    31681 ggcttcaagc gccagatcgc cccggtaatt acggcttttc agcggcgaaa cggtcattgg
    31741 ccagacccag ttgcacaggg tgacggcgtt atgcaaacag acccgcaaca gcgaccactt
    31801 tgcatattta atttttgcgt cattgattgg cgtgtagttt ttggtttcat cgtaggcatc
    31861 ttcagaacca aaaagctctt tcgccacttc acgctgcagt ttcaatggca aacccgaacc
    31921 aataaaattg atatgggtat gcgtcatgca gtcacggttg aacatacagt taacaatgga
    31981 accgacctgc gctgacgctt cattggcatg gtgaaccgga tagccaaacg gcgaccagag
    32041 tttatttttt gcgtagcccc agtactcttc acccaaattc cagcgttctg cgatggcata
    32101 tgaaccatca gccaggtgac tcagctcacc cacacgatgc gccagaaggt agtaaaaatc
    32161 tttaatgaaa ttaacgtcac ccgcttccag ttgatcccag tgaatttccg catactcttc
    32221 agctggcaga acacgcttga acaccccttt gctgtaacaa taagtaaagt cgcgatgcag
    32281 ctgcccgtag ttacaccata ggccgtagtc atcgaacaaa ttcagaccaa ccaggttacc
    32341 aatcacacga ccatcgtctt tatcttcaaa atctttcggc ccgttcggga agatggtggt
    32401 atggacaaag ttggctacac aagtgttacc acctgtgctg ggcacgccaa actctttcac
    32461 ccgaggaata ttcatttggg tcatacaacg gatcgggcaa gagtggcagc cactcatttt
    32521 tactgtgtat ttctctgccg ccggacctaa gtcaaaaacg gatttatagg tacgaaagcc
    32581 gaccgtgttc tgattgcctg gcggaatttc ccccgtttca atcggaccac cttcagccgc
    32641 gccccaaaac agccctttac gtgctgtcca acgtgacttg ggatctgaat actctgccca
    32701 cgattgcggc gtacttggca cgacatggtt gttattcgca ccaataagtt cagtcatcat
    32761 gtaatcgttg agacgcttca tctcctgacg atcggcaatg ttgacccctt tcgtcccttc
    32821 aaccgcaatc gctttcaggt ttttcgaacc cataattgcg ccagttcccg caccgccgct
    32881 gtggttacgg ctattcaaca tgccagagag aggaacaagg ttttccccag cctgaccaat
    32941 agccgccaca caggtttccg gactggtcaa tcgacaaatt tcttccgtcg ttgcgcgcgt
    33001 cccttttccc cataagaaat cggctttttc caggctaact ttgtcatctt taatcttcag
    33061 ccataccggt gatttcgcct tcccttcgat aataatgacg tcgtatccag cgaatttcat
    33121 ttgcgctgca aaaaagccac ccatatgggc atcgacaact aaatttcctt tggtaaaagt
    33181 agaaagtgag gtgatattta cgcgagaact acagggggca ccagatccag ttaatgggcc
    33241 ggtagcaaag actaatttat tcgcttcatc gaaaggtttc gtgcctggcg gtacttcgtc
    33301 atacataatt ttgtagccga agcccatgcc accgacaaaa cttttaaact tactggaatc
    33361 ttcgagggta atatttcctg tcgtgagatt gactcttaat atattacctg tccaaccgtt
    33421 agccatgatt ttttcctttg caagattcac acagtaatat ctttccactc gataattttt
    33481 aacgcccctg ttgggcaggc gtttgcgcat tcaccgcata acacacattt cgaggatttt
    33541 ttactttcgg tatttacggt ggccatcatc cacgggcatg ccgtagtaca ggcgctacag
    33601 ccaatacagc gtttatgatc gacggtaata cagccttctt tctgctgcca ggtaatcgca
    33661 ccaatcgggc agacgttcat gcattgcggc tctttgcatt gacggcaggt gtccgcggta
    33721 tagttgagat cgccatacag gccgccgcca gagccaaccc cgttgtcgcc aaagaaataa
    33781 ttgcggtgga ttttgatacg ggagaagaat gtccctactg agccatcgtt gaagttggta
    33841 caagagattt cacagcggtg gcaacccgtg catcgcgctc gctgggtcac cagcacccct
    33901 ttgggtgtat ttatcaaccc tactgtgccg ctatcaatat cttcctgttt gcaacccaaa
    33961 agtgacagta acgcaggggc aattgttaga ccagcaaggc cttttcctga tatacgcaag
    34021 aattctaatc gcgttaagcc aatatctaat agtggacgat caaccgggtt catttatttc
    34081 gcatcctctt cgcaaacaca gtgctgacaa ttcagcagcc tgatattaag cattcaacta
    34141 atacgctgct gactctgtcc ccaacaccag atattttccc taacccgata gtgtatgaga
    34201 aaagatttaa cgccgataaa ctaatccttt atggttagga gaaaaaataa tgtgattatt
    34261 ctgagccctt aacattgatc gttatcaatt aaaattacaa agcaagaaat tataagtgaa
    34321 ctgatattta ttatcatttg aaataaattt aacttaagcg acaattattt gtgacgttca
    34381 ttgcagaaaa taaaaataac attatcgcta taaattaaaa tacataccga aattcgtata
    34441 atttcagcag ttgttattta gaatgattaa ctggaccgca actgaagcaa gaatatcagg
    34501 atgaagctta ccgcctcatc ctgaatggtc attttaccag tgcgttaatg acggtttcca
    34561 tcgcctgctt ttccagctca ctgcgctgaa tgcgtttatt ggctaatgct gtacgtaata
    34621 cgcctgcgat aacaatatgc tttggttcct gccccagatc gcgcatttcc agcacaactt
    34681 taccgacaac tctgcacatt tctcggtaca gttcatcctc ttttgtccga tttcccattc
    34741 tttataccta tttatcatgc caactatcag catatatcaa tctaaccaat taaacaaaaa
    34801 tcaaacaaaa tcagacaaat aacgcgataa attattttaa ctgtagcaat tgagcgatga
    34861 tatatttata caccggatga actttcactt atcctcacac tgacaacttc ggcaccagac
    34921 gttgcgcaaa cagtgaagtt tttgcgtaac cttttccctg gaacgttaaa tctttgataa
    34981 caatttattg tctaacaagt tgtatatttt ttgaaacgct gtttttgttt tccttttgga
    35041 ttaatttcag cgtataatgc gcgccaattg actcttgaat ggtttcagca ctttggactg
    35101 tagaactcaa cgactcaaaa acaggcactc acgttgggct gagacacaag cacacattcc
    35161 tctgcacgct ttttcgatgt cacctatcct tagagcgagg caccaccact ttcgtaatac
    35221 cggattcgct ttccggcagt gcgcccagaa agcaagtttc tcccatcctt ctcaacttaa
    35281 agactaagac tgtcatgaaa aagaccaaaa ttgtttgcac catcggaccg aaaaccgaat
    35341 ctgaagagat gttagctaaa atgctggacg ctggcatgaa cgttatgcgt ctgaacttct
    35401 ctcatggtga ctatgcagaa cacggtcagc gcattcagaa tctgcgcaac gtgatgagca
    35461 aaactggtaa aaccgccgct atcctgcttg ataccaaagg tccggaaatc cgcaccatga
    35521 aactggaagg cggtaacgac gtttctctga aagctggtca gacctttact ttcaccactg
    35581 ataaatctgt tatcggcaac agcgaaatgg ttgcggtaac gtatgaaggt ttcactactg
    35641 acctgtctgt tggcaacacc gtactggttg acgatggtct gatcggtatg gaagttaccg
    35701 ccattgaagg taacaaagtt atctgtaaag tgctgaacaa cggtgacctg ggcgaaaaca
    35761 aaggtgtgaa cctgcctggc gtttccattg ctctgccagc actggctgaa aaagacaaac
    35821 aggacctgat ctttggttgc gaacaaggcg tagactttgt tgctgcttcc tttattcgta
    35881 agcgttctga cgttatcgaa atccgtgagc acctgaaagc gcacggcggc gaaaacatcc
    35941 acatcatctc caaaatcgaa aaccaggaag gcctcaacaa cttcgacgaa atcctcgaag
    36001 cgtctgacgg tatcatggtt gcgcgtggcg acctgggtgt tgaaatcccg gttgaagaag
    36061 ttatcttcgc tcagaagatg atgatcgaaa aatgtatccg tgcacgtaaa gtcgttatta
    36121 ctgcgactca aatgctggat tccatgatca aaaacccacg tccaacccgc gcagaagcag
    36181 gtgacgttgc taacgccatt ctggatggta ctgacgcagt tatgctgtct ggtgaatccg
    36241 caaaaggtaa gtatccgctg gaagctgttt ctattatggc gaccatttgc gaacgtactg
    36301 accgtgtaat gaccagccgt ctggatttca acaacgacag ccgtaaactg cgtatcactg
    36361 aagcagtttg ccgtggtgca gttgaaactg cagaaaaact ggatgctccg ctgatcgttg
    36421 ttgcgaccca gggcggtaaa tctgcacgtg cggtgcgtaa atacttcccg gatgcaacca
    36481 tcctggcgct gaccaccaac gaaaaaactg cacatcagct ggtgctgagc aaaggcgttg
    36541 tgccgcagtt ggtgaaagaa atcacctcta ctgatgactt ctaccgtctg ggtaaagaac
    36601 tagctttgca gagcggtctg gctcagaaag gtgatgttgt ggtcatggtt tctggcgcac
    36661 tggtaccgag cggcactact aacaccgcat ctgttcacgt cctgtaatat tgcttttgtg
    36721 aatt
//
LOCUS       DAZFXT010000039        36680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.039, whole genome shotgun sequence.
ACCESSION   DAZFXT010000039 DAZFXT010000000
VERSION     DAZFXT010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.039"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            393..1610
                     /gene="alaA"
                     /locus_tag="ACHKW0_002029"
     CDS             393..1610
                     /gene="alaA"
                     /locus_tag="ACHKW0_002029"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HFO7474863.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1694..2293
                     /gene="yfbR"
                     /locus_tag="ACHKW0_002030"
     CDS             1694..2293
                     /gene="yfbR"
                     /locus_tag="ACHKW0_002030"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002953 - 5'-deoxynucleotidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7474864.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELQDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2352..4184)
                     /locus_tag="ACHKW0_002031"
     CDS             complement(2352..4184)
                     /locus_tag="ACHKW0_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HFO7474865.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4271..4921)
                     /gene="hxpA"
                     /locus_tag="ACHKW0_002032"
     CDS             complement(4271..4921)
                     /gene="hxpA"
                     /locus_tag="ACHKW0_002032"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HFO7474866.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4932..5426)
                     /locus_tag="ACHKW0_002033"
     CDS             complement(4932..5426)
                     /locus_tag="ACHKW0_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HFO7474867.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5509..5964)
                     /gene="yfbV"
                     /locus_tag="ACHKW0_002034"
     CDS             complement(5509..5964)
                     /gene="yfbV"
                     /locus_tag="ACHKW0_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminus macrodomain insulation protein YfbV"
                     /protein_id="HFO7474868.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6302..7504
                     /gene="ackA"
                     /locus_tag="ACHKW0_002035"
     CDS             6302..7504
                     /gene="ackA"
                     /locus_tag="ACHKW0_002035"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HFO7474869.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7579..9723
                     /gene="pta"
                     /locus_tag="ACHKW0_002036"
     CDS             7579..9723
                     /gene="pta"
                     /locus_tag="ACHKW0_002036"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7474870.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9913..11433
                     /gene="yfcC"
                     /locus_tag="ACHKW0_002037"
     CDS             9913..11433
                     /gene="yfcC"
                     /locus_tag="ACHKW0_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HFO7474871.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11466..12008)
                     /gene="yfcD"
                     /locus_tag="ACHKW0_002038"
     CDS             complement(11466..12008)
                     /gene="yfcD"
                     /locus_tag="ACHKW0_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HFO7474872.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12066..12617)
                     /gene="yfcE"
                     /locus_tag="ACHKW0_002039"
     CDS             complement(12066..12617)
                     /gene="yfcE"
                     /locus_tag="ACHKW0_002039"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HFO7474873.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12673..13317)
                     /gene="yfcF"
                     /locus_tag="ACHKW0_002040"
     CDS             complement(12673..13317)
                     /gene="yfcF"
                     /locus_tag="ACHKW0_002040"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HFO7474874.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13453..14100
                     /gene="yfcG"
                     /locus_tag="ACHKW0_002041"
     CDS             13453..14100
                     /gene="yfcG"
                     /locus_tag="ACHKW0_002041"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HFO7474875.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14157..14519
                     /gene="folX"
                     /locus_tag="ACHKW0_002042"
     CDS             14157..14519
                     /gene="folX"
                     /locus_tag="ACHKW0_002042"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HFO7474876.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14540..15433
                     /locus_tag="ACHKW0_002043"
     CDS             14540..15433
                     /locus_tag="ACHKW0_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HFO7474877.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15481..16371)
                     /gene="rpnB"
                     /locus_tag="ACHKW0_002044"
     CDS             complement(15481..16371)
                     /gene="rpnB"
                     /locus_tag="ACHKW0_002044"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HFO7474878.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIAHKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(16568..17341)
                     /gene="hisP"
                     /locus_tag="ACHKW0_002045"
     CDS             complement(16568..17341)
                     /gene="hisP"
                     /locus_tag="ACHKW0_002045"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HFO7474879.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17349..18065)
                     /locus_tag="ACHKW0_002046"
     CDS             complement(17349..18065)
                     /locus_tag="ACHKW0_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7474880.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(18062..18748)
                     /gene="hisQ"
                     /locus_tag="ACHKW0_002047"
     CDS             complement(18062..18748)
                     /gene="hisQ"
                     /locus_tag="ACHKW0_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HFO7474881.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18838..19620)
                     /gene="hisJ"
                     /locus_tag="ACHKW0_002048"
     CDS             complement(18838..19620)
                     /gene="hisJ"
                     /locus_tag="ACHKW0_002048"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HFO7474882.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19841..20623)
                     /gene="argT"
                     /locus_tag="ACHKW0_002049"
     CDS             complement(19841..20623)
                     /gene="argT"
                     /locus_tag="ACHKW0_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HFO7474883.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20889..21458)
                     /gene="ubiX"
                     /locus_tag="ACHKW0_002050"
     CDS             complement(20889..21458)
                     /gene="ubiX"
                     /locus_tag="ACHKW0_002050"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HFO7474884.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21553..23070)
                     /gene="purF"
                     /locus_tag="ACHKW0_002051"
     CDS             complement(21553..23070)
                     /gene="purF"
                     /locus_tag="ACHKW0_002051"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HFO7474885.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(23107..23595)
                     /gene="cvpA"
                     /locus_tag="ACHKW0_002052"
     CDS             complement(23107..23595)
                     /gene="cvpA"
                     /locus_tag="ACHKW0_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HFO7474886.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23854..24516)
                     /gene="dedD"
                     /locus_tag="ACHKW0_002053"
     CDS             complement(23854..24516)
                     /gene="dedD"
                     /locus_tag="ACHKW0_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_process: GO:0032506 - cytokinetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HFO7474887.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24506..25774)
                     /gene="folC"
                     /locus_tag="ACHKW0_002054"
     CDS             complement(24506..25774)
                     /gene="folC"
                     /locus_tag="ACHKW0_002054"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HFO7474888.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25844..26758)
                     /gene="accD"
                     /locus_tag="ACHKW0_002055"
     CDS             complement(25844..26758)
                     /gene="accD"
                     /locus_tag="ACHKW0_002055"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HFO7474889.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26914..27573)
                     /locus_tag="ACHKW0_002056"
     CDS             complement(26914..27573)
                     /locus_tag="ACHKW0_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7474890.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            complement(27656..28468)
                     /gene="truA"
                     /locus_tag="ACHKW0_002057"
     CDS             complement(27656..28468)
                     /gene="truA"
                     /locus_tag="ACHKW0_002057"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HFO7474891.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28468..29481)
                     /locus_tag="ACHKW0_002058"
     CDS             complement(28468..29481)
                     /locus_tag="ACHKW0_002058"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HFO7474892.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29547..30683)
                     /gene="pdxB"
                     /locus_tag="ACHKW0_002059"
     CDS             complement(29547..30683)
                     /gene="pdxB"
                     /locus_tag="ACHKW0_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033711 - 4-phosphoerythronate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HFO7474893.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30782..31777
                     /gene="flk"
                     /locus_tag="ACHKW0_002060"
     CDS             30782..31777
                     /gene="flk"
                     /locus_tag="ACHKW0_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HFO7474894.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31774..32952)
                     /locus_tag="ACHKW0_002061"
     CDS             complement(31774..32952)
                     /locus_tag="ACHKW0_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474895.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33236..34456)
                     /gene="fabB"
                     /locus_tag="ACHKW0_002062"
     CDS             complement(33236..34456)
                     /gene="fabB"
                     /locus_tag="ACHKW0_002062"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HFO7474896.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFSADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34615..36621
                     /gene="mnmC"
                     /locus_tag="ACHKW0_002063"
     CDS             34615..36621
                     /gene="mnmC"
                     /locus_tag="ACHKW0_002063"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HFO7474897.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT         9084 a         9979 c         9098 g         8519 t
ORIGIN      
        1 ttcattaatt ccgcttaaca atagtttcac accttgcccc cattgcgaca cctcggaaat
       61 catcacgctg ttaaactctc cgtaatgcgg aaatatcatc atcgcgtttc attagtgaat
      121 tcttaaatga ggcattttca cacaattatc ttacagataa aaaaaccaga cttacaatta
      181 agaatcagaa caattcacta tataacattg catgtaaagc atatacacct cattattttg
      241 tcattattaa gttattaaca gcacaatcga gccttccact ctggcaaaat cttattctgc
      301 agaccttcaa aacaccgtcc tgggggagta cattgttcta agctgacttc cacggcaggg
      361 agtggcgata acagcaaaaa aggtcaagat tcatgtcccc cattgaaaaa tccagcaaat
      421 tagagaatgt ctgttatgac atccgtggtc cggtgctgaa agaagcaaaa cgcctggaag
      481 aagaaggtaa caaggtactg aaactgaaca tcggcaaccc agccccgttc ggttttgacg
      541 cgccagatga aatcctcgtt gacgtgatac gcaacctgcc taccgctcaa gggtattgcg
      601 attccaaagg gctttactcc gcgcgtaaag ccatcatgca gcactaccag gctcgtggca
      661 tgcgtgatgt taccgtggaa gatatttaca tcggcaatgg tgtatcggag cttatcgttc
      721 aggcaatgca ggcattgctg aacagcgggg acgaaatgtt ggttcctgca ccagattacc
      781 cactatggac cgcggcggtt tcgctttcca gcggtaaagc ggtgcattat ctttgcgatg
      841 aatcctctga ctggttcccg gacctcgatg atattcgcgc taaaattacg cctcgtacgc
      901 gtgggatcgt tattatcaac ccaaataacc caaccggcgc ggtatattcc aaagagcttt
      961 taatggagat tgtggagatt gcacgtcagc ataatctcat tatcttcgcc gatgaaattt
     1021 atgacaaaat tctctacgac gacgctgagc atcactcaat tgcgccgctg gcacctgacc
     1081 tgctgaccat tacctttaac ggactgtcga aaacgtaccg cgttgcaggc ttccgtcagg
     1141 ggtggatggt gttgaacggg ccgaaaaaac acgccaaagg ctacatcgaa ggtctggaaa
     1201 tgctggcttc aatgcgcctg tgtgctaacg ttcctgcgca acacgccatt cagaccgcgc
     1261 tgggtggtta tcagagcatc agtgaattta ttacccctgg cggtcgtctt tatgagcagc
     1321 gtaaccgcgc gtgggaactg atcaacgata ttccgggcgt ttcctgcgtg aaacctcgtg
     1381 gtgcgctgta tatgttcccg aaaatcgacg ccaaacgctt taacattcac gacgatcaga
     1441 aaatggtgct ggatttcctg ttgcaggaaa aagtcctgtt ggtgcaaggg acggcattca
     1501 actggccgtg gccggatcac ttccgcattg tcactctgcc gcgtgtcgat gatatcgagc
     1561 tgtctttgag taagttcgcg cgtttccttt ctggttatca tcagctgtaa tcttaatttc
     1621 actgccggag attgcatccg gcagtgttat cccgccacaa tgacctgatg atgtcatcat
     1681 acgtaaggtc actatgaaac agagccattt ttttgcccat ctctcacgcc tgaaactcat
     1741 taaccgctgg ccgctaatgc gcaacgtgcg gacggaaaat gtgtccgaac acagtttgca
     1801 ggtagcgatg gtcgcccatg cgctggcagc tatcaaaaat cgtaaatttg gcggtaatgt
     1861 caacgccgaa cgtatcgctt tactggcgat gtaccacgat gcctcagaag tgctcaccgg
     1921 cgatctccct actccggtga aatacttcaa ttcgcaaatc gctcaggagt acaaggctat
     1981 tgaaaaaatc gctcagcaaa aactggtcga tatggttccg gaagagctgc aggatatctt
     2041 tgcgccgtta attgacgagc atgcatatag cgatgaagaa aaatcgttgg tgaaacaggc
     2101 agatgcactg tgtgcatatc tgaaatgtct ggaagaactc gcggccggaa ataatgaatt
     2161 cttgctggca aaaacgcgac tggaagcgac gcttgaagcg cgtcgcagcc aggagatgga
     2221 ctacttcatg gaagtatttg ttcccagctt ccatctttcg ctcgatgaga ttagccagga
     2281 ttcaccgctg taagcagccg gagtctgcgt cgcatcaggc aataagcgcc ggatgcgaca
     2341 tcaggctctt gtcaaaacgg aaacagcatc gggatcatca ccacacaaac cgccatcacg
     2401 ataatggtga acggtacccc caacttcaca aagtcactga agctgtaatt tcccggacct
     2461 aaaaccagtg tgttaaccgg tgaagaaacc ggtgtcataa aggcggcaga tgctgccatc
     2521 gccacgacca tcgcgaatgg ataaggcgac acccccatcg ttttggcagc agccagcgca
     2581 atcggagcca tcaacaccgc cgtcgcggta ttagagataa acagcccaat aaccgccgac
     2641 aaaacaaaca aacaccccag catcatatgt ggcccgtaac cgccgccaat gtccatcagc
     2701 cctttcaccg ccagcgcgac acctcccgtt ttctgtaatg ccacagcaaa cggcatcatc
     2761 ccaacgatca aaataatgct cggccagtga atggatttat aggcgctttc ggcatcgata
     2821 cagcggaatt tccccatcag caggcaggcg atgatagcgg caacaggatt aggaatttca
     2881 tcggtcagca ttaacgccac cattagcacc agacagaaaa tggcatgggg tgcctggctg
     2941 tgcgcgggcg atgcttcact cacctcttcc ggtaagttca gcgctacgaa gtcgcggccc
     3001 tgttttgcca gcataccgat cagtttccag ttaccaacaa ccaggatgat atcgcccagc
     3061 agcagaggct catccgccag cgaaccttcc agcgccacgc cattgcgctt tagccccacc
     3121 acattcagtc cgtagcgggt acgaaaacca atttcgcgca ccgatttacc aatcagttct
     3181 gactcaggaa ttaatgaaat ctctgccatg cccacatcaa gggcctggtc agaaaaatac
     3241 tcgccgcgca gtaccatcgg ctccagcaat tgctcactac aaaattgccg gagatcgaca
     3301 tcagccgcag acatatcaat aagcaaaacg tcacgcgcgc gaaattcaga aaccccatta
     3361 acgttcacga taacgcgacg aaaacgccgc cagcgttcaa caccgatgac gttagcgcca
     3421 taacgctcac gtaatttgag atcgtccagc cgttgaccaa tcattggcga tccggggcga
     3481 atagccagac gtcgcgcacg cccggtcagt cgatattcac ggataagatc gcgaaaggtt
     3541 cgacgcgtcc agccttcgcg ctgcggggtc tgggtatccc ctttcagcat gaaacgcatc
     3601 actaacatat acaaaatacc cagcaccagc acgaccaggc caataggtgt tacgctaaag
     3661 aaactgaagc cgtgatagcc ttcacgcagc aattcactgt tgactaccag gttcggcggc
     3721 gtcgccacca gcgtcatcat gccgctaatc agcccggcaa aacttaacgg catcatcaga
     3781 cgtgacggcg aagtttgcat acgcatggca acgcttaaca ccacgggaat aaagatagcg
     3841 acaacgccgg ttgaactcat aaacgcgcca agccccgcga cggtcagcat caacaaaacc
     3901 aacattttga tttcactgtt gcccgccact ttgaccagcc atgttcccat tacggtggca
     3961 acaccggtac ggaccaaacc atcgccaata ataaacaagg cggcaatcag gacaacgtta
     4021 ggatcagaaa agccggaaaa tacttcgggg accgtcagcg ttccgcttaa tgcaaacgcg
     4081 acaataacaa acaaagcgac cgcatccata cgcactctgc ccgtcgcaaa caagacgatg
     4141 gcaacggcca gtaatgaaag aacccaaatc aattcaccgt tcacaactta tccttgttaa
     4201 ttgaggggga tgacttgatt ctgccataaa aaagccccga cgagacgggg ctaaatcatg
     4261 atcaggtgtt tcactgaata ataacatcgc catttggctg tttggtcaca gtaatttgct
     4321 ccagactgtg gaggaccaaa tcgacctcat tcaggcgcgg ggtatctgcc ggagcgttaa
     4381 ccgcaatgac atggcaaccc gccgccaggc cagaaagcac gccagcggga gcatcttcca
     4441 ccaccacaca ctcctgcggc gcaagcccca gcagctgcgc acctaacaga tacgcatcag
     4501 gttctggttt tccgcgcttc actcgctcag cggttacaaa cacctctggt gcgggaagcc
     4561 cagctatttt atggcgcgct cgcgctaccg gcatggagcc agaagtcaca atggcccacg
     4621 gaatacctgc tttattcaaa tgattgagta aggcgattgc ccccggaagc gcggtaatac
     4681 cttcggtttc cgtggcctcg atgtgctcca gacgcgtaaa ctcggcggca atatcagcct
     4741 cggatttgcc cgccataaaa tggcgcaaag aggtgatcgc ctgtttaccg tgaatgaaag
     4801 ccagcacctc ttccggtgct aacccatgac gtctggccca gttgctccac gcccgttcta
     4861 ccgcaggcag ggaatccacc agcgttccat caagatcaaa cagaaaacct ttgcaccgca
     4921 cacgggcctc ctcaggcgtt gataatttga ttaatttcgt tggcgctcaa atggtactga
     4981 cgcgggcagg catgccacac attaagcatg cgctgatatt tttcccacat tggcgtctgg
     5041 gcgttaaaac cgtgagttcc ggcgtcaaaa tgggtatagc gcccttccac attaaccata
     5101 aagcggacat aaccgaggta acgtgcttca gtggcggcgt caaagccgag gaaggtgaca
     5161 cgacgttcat cgatggattg ctggtcctgc aaattagacc aggaaacatg caacgcatga
     5221 tacatctcca taatgtcgat gatagtgcgg caggtttctt ctttcagctc gccaaactcg
     5281 cgatccaatt cacgcatctg taatccgtaa ccacgctcaa taattgtttg caggcgacgg
     5341 taacgttcag catttgccgg atcgagcata gtcatcatct tgtactgatt agacaaaata
     5401 agacgttgcg cgttggtcat ttccattgtt gactcctgta tcactatact acggtgaaaa
     5461 aaaagaaggc tgagtatgcc ttcttttata tgcgtaatca ggggtcaatt acaaatcatc
     5521 aaggaaagtt ttatccagtt gtttgaaggc gcgcttaagc gtgtcagcta atgcctggta
     5581 atcaggcttg ccttcaacgg gtgccaacac ctgtccagac tcctgcaatt taccgcgaac
     5641 ttcataaaac cagttaagga ttgcaggggg taatggcgtt acagaacgct tgcccagcca
     5701 ccacaatccc tgcatgggta aacttaaggc gaacagcgca gtggcaactg ccggcccaag
     5761 ctgaccgccc agggcaatct gccagcagag agtaaatacg gcgatcggcg gcataaaacg
     5821 gatcgcataa cgcgtcatct tgataacgcg attttcgaca aagaccgggg caaggcgttt
     5881 ttccagcggc cacgtctttg agtaatgctg tccccggcga aacaagctaa aaaaattaac
     5941 agaacgatta tccggcgttg acatgcttca cctcaacttc acatataaag attcaaaaat
     6001 ttgtgcaaat tcacaactca gcgggacaac gttcaaaaca ttttgtcttc catacccact
     6061 atcaggtatc ctttagcagc ctgaaggcct aagtagtaca tattcattga gtcgtcaaat
     6121 tcatatacat tatgccattg gctgaaaatt acgcaaaatg gcatagactc aagatatttc
     6181 ttccatcatg caaaaaaaat ttgcagtgca tgatgttaat cataaatgtc ggtgtcatca
     6241 tgcgctacgc tctatggctc cctgacgttt ttttagccac gtatcaatta taggtacttc
     6301 catgtcgagt aagttagtac tggttctgaa ctgcggtagt tcttcactga aatttgccat
     6361 catcgatgca gtaaatggtg aagagtacct ttctggttta gccgaatgtt tccacctgcc
     6421 cgaagcacgt atcaaatgga aaatggacgg caataaacag gaagcggctt taggtgcagg
     6481 cgccgctcac agcgaagcgc tcaactttat cgttaatact attctggcac aaaaaccaga
     6541 actgtctgcg cagctgactg ctatcggtca ccgtatcgta cacggcggcg aaaagtatac
     6601 cagctccgta gtgatcgatg agtctgttat tcagggtatc aaagatgcag cttcttttgc
     6661 accgctgcac aacccggctc acctgatcgg tatcgaagaa gctctgaaat ctttcccaca
     6721 gctgaaagac aaaaacgttg ctgtatttga caccgcgttc caccagacta tgccggaaga
     6781 gtcttacctc tacgccctgc cgtacaacct gtacaaagag cacggcatcc gtcgttacgg
     6841 cgcgcacggc accagccact tctatgtaac ccaggaagcg gcaaaaatgc tgaacaaacc
     6901 ggtagaagaa ctgaacatca tcacctgcca cctgggcaac ggtggttccg tttctgctat
     6961 ccgcaacggt aaatgcgttg acacctctat gggcctgacc ccgctggaag gtctggtcat
     7021 gggtacccgt tctggtgata tcgatccggc gatcatcttc cacctgcacg acaccctggg
     7081 catgagcgtt gacgcaatca acaaactgct gaccaaagag tctggcctgc tgggtctgac
     7141 cgaagtgacc agcgactgcc gctatgttga agacaactac gcgacgaaag aagacgcgaa
     7201 gcgcgcaatg gacgtttact gccaccgcct ggcgaaatac atcggtgcct acactgcgct
     7261 gatggatggt cgtctggacg ctgttgtatt cactggtggt atcggtgaaa atgccgcgat
     7321 ggttcgtgaa ctgtctctgg gcaaactggg cgtgctgggc tttgaagttg atcatgaacg
     7381 caacctggct gcacgtttcg gcaaatctgg tttcatcaac aaagaaggta cccgtcctgc
     7441 ggtggttatc ccaaccaacg aagaactggt tatcgcgcaa gacgcgagcc gcctgactgc
     7501 ctgatttcac accgccagct cagctggcgg tgctgttttg taacccgcca aatcggcggt
     7561 aacgaaagag gataaaccgt gtcccgtatt attatgctga tccctaccgg aaccagcgtc
     7621 ggtctgacca gcgtcagcct tggcgtgatc cgtgcaatgg aacgcaaagg cgttcgtctg
     7681 agcgttttca aacctatcgc tcagccgcgt accggtggcg atgcgcccga tcagactacg
     7741 actatcgtgc gtgcgaactc ttccaccacg acggccgctg aaccgctgaa aatgagctac
     7801 gttgaaggtc tgctttccag caatcagaaa gatgtgctga tggaagagat cgtcgcaaac
     7861 taccacgcta acaccaaaga cgctgaagtc gttctggttg aaggtctggt cccgacacgt
     7921 aagcaccagt ttgcccagtc tctgaactac gaaatcgcta aaacgctgaa tgcggaaatc
     7981 gtcttcgtta tgtctcaggg cactgacacc ccggaacagc tgaaagagcg tatcgaactg
     8041 acccgcaaca gcttcggcgg tgccaaaaac accaacatca ccggcgttat cgttaacaaa
     8101 ctgaacgcac cggttgatga acagggtcgt actcgcccgg atctgtccga gattttcgac
     8161 gactcttcca aagctaaagt aaacaatgtt gatccggcga agctgcaaga atccagcccg
     8221 ctgccggttc tcggcgctgt gccgtggagc tttgacctga tcgcgactcg tgcgatcgat
     8281 atggctcgcc acctgaatgc gaccatcatc aacgaaggcg acatcaatac tcgccgcgtt
     8341 aaatccgtca ctttctgcgc acgcagcatt ccgcacatgc tggagcactt ccgtgccggt
     8401 tctctgctgg tgacttccgc agaccgtcct gacgtgctgg tggccgcttg cctggcagcc
     8461 atgaacggcg tagaaatcgg tgccctgctg ctgactggcg gctacgaaat ggacgcgcgc
     8521 atttctaaac tgtgcgaacg tgctttcgct accggcctgc cggtatttat ggtgaacacc
     8581 aacacctggc agacctctct gagcctgcag agcttcaacc tggaagttcc ggttgacgat
     8641 cacgagcgta tcgagaaagt tcaggaatac gttgctaact acatcaacgc tgactggatc
     8701 gaatctctga ctgccacttc tgagcgcagc cgtcgtctgt ctccgcctgc gttccgttat
     8761 cagctgactg aacttgcgcg caaagcgggc aaacgtatcg tactgccgga aggtgacgaa
     8821 ccgcgtaccg ttaaagcagc cgctatctgt gctgaacgtg gtatcgcaac ttgcgtactg
     8881 ctgggtaatc cggcagagat caaccgtgtt gcagcgtctc agggtgtaga actgggtgca
     8941 gggattgaaa tcgttgatcc agaagtggtt cgcgaaagct atgttggtcg tctggtcgaa
     9001 ctgcgtaaga acaaaggcat gaccgaaacc gttgcccgcg aacagttgga agacaacgtg
     9061 gtgctcggta cgctgatgct ggaacaggat gaagttgatg gtctggtttc cggtgctgtt
     9121 cacactaccg caaacaccat ccgtccgccg ctgcagctga tcaaaactgc accgggcagc
     9181 tccctggtat cttccgtgtt cttcatgctg ctgccggaac aggtttacgt ttacggtgac
     9241 tgtgcgatca acccggatcc gaccgctgaa cagctggcag aaatcgcgat tcagtccgct
     9301 gattccgctg cggccttcgg tatcgaaccg cgcgttgcta tgctctccta ctccaccggt
     9361 acttctggtg caggtagcga cgtagaaaaa gttcgcgaag caactcgtct ggcgcaggaa
     9421 aaacgtcctg acctgatgat cgacggtccg ctgcagtacg acgctgcggt aatggctgac
     9481 gttgcgaaat ccaaagcgcc gaactctccg gttgcaggtc gcgctaccgt gttcatcttc
     9541 ccggatctga acaccggtaa caccacctac aaagcggtac agcgttctgc cgacctgatc
     9601 tccatcgggc cgatgctgca gggtatgcgc aagccggtta acgacctgtc ccgtggcgca
     9661 ctggttgacg atatcgtcta caccatcgcg ctgactgcga ttcagtctgc acagcagcag
     9721 taatctcgtc atcatccgca gctttgcgct gcggatatct gaaccggaaa taatcactat
     9781 ttccggtttt ttattctctt aatttgcatt aatcctttct gattatcttg cttaactgcg
     9841 ctgcatcaat gaattgcgcc atctcacttt gcatacttac cactttgttt tgtgcaaggg
     9901 aatatttgcg ctatgtccgc aatcactgaa tccaaaccaa caagaagatg ggcaatgccc
     9961 gatacgttgg tgattatctt ttttgttgct attttaacca gccttgccac ctgggtagtt
    10021 ccggtgggga tgtttgacag tcaggaagtg cagtatcagg ttgatggtca aacaaaaaca
    10081 cgcaaagtcg tagatccaca ctcatttcgc attctgacta acgaagcagg cgaacctgag
    10141 tatcaccgcg tacagctgtt cacgacgggc gatgaacgcc cgggcctgat gaacttcccg
    10201 tttgaaggat taacctcagg atcgaaatac gggacagccg ttggcatcat catgtttatg
    10261 ctggtgattg gcggcgcgtt tggcattgtg atgcgtacag gaaccattga taacggtatc
    10321 ctggcgctta ttcgccatac ccgcgggaat gaaattctct ttattcctgc gctgtttatt
    10381 ctgttttcac ttggcggcgc ggtatttggt atgggagaag aggccgtcgc ctttgccatt
    10441 atcatcgcac cgctaatggt ccggctgggc tatgacagta ttaccaccgt cctggtgacc
    10501 tatattgcca cgcaaatcgg ttttgccagt tcgtggatga acccgttttg tgtggtcgtt
    10561 gctcagggga ttgccggcgt tccggtgctt tctggctccg ggttgcgcat cgtggtgtgg
    10621 gttatcgcca ctctgattgg cctgatcttt accatggtgt acgcctcacg agtgaaaaag
    10681 aatcctcttc tgtcacgcgt gcatgagtcc gaccgcttct ttcgtgaaaa gcaggctgat
    10741 gttgaacaac gtccgtttac ctttggtgac tggctggtat tgattgtcct gaccgccgta
    10801 atggtctggg tgatttgggg cgtgatcgtt aatgcctggt ttattccaga aattgccagc
    10861 cagttcttca ccatgggtct ggtgattggc atcatcggcg ttgttttccg ccttaacggc
    10921 atgacggtta ataccatggc ttcatccttt accgaagggg cgcgaatgat gatcgcccct
    10981 gccctgctgg tgggtttcgc caaagggatt ttgctgctgg tcggtaatgg tgaagcgggt
    11041 gatgccagcg tgttaaatac catcctcaac agcattgcca atgccattag cggtctggac
    11101 aacgcggtcg cggcctggtt tatgttgctc ttccaggcgg tatttaattt cttcgtgacg
    11161 tccggttctg gtcaggcggc gttaaccatg ccgttactgg caccgcttgg cgatctggtc
    11221 ggtgttaacc gccaggttac cgtgctggca ttccagtttg gtgatggctt cagtcacatc
    11281 atttacccaa cctcagcttc gttaatggca actctcggtg tatgcagggt ggacttccgt
    11341 aactggctga aggtgggcgc gaccctactt ggactgctgt ttattatgtc cagcgtcgta
    11401 gtgatcggcg ctcagttgat gggctaccac taaaaatgtt aagagccgca attgcggctc
    11461 ttttttcatt ctgccgtttc agtctctacg gcttcatttt tggcattgcg cttcatccac
    11521 aacgccagcg ctttcagcga gtctggagtg aactcatcgc agcgtgcggt gatttcttcc
    11581 ggcgtcagcc agcaaacttc actgacttca tcttcctgta gggcgaaggg accgtgagag
    11641 acgcagctga acaacgcgcc ccagacacgg caatttttat cttcgaaata gaactgcccg
    11701 tgctcggcaa aggggacacc ggcaatgccc agctcttctt ccgcttcgcg acgcgcggat
    11761 tccagcagtt gctcatcggc ctgaaccaca ccgcctgcgg tcgcatctaa catgccgggt
    11821 aaaaagtctt ttgtctcggt acgacgctgg accagaattt tgcccatgcc atcatgcacg
    11881 acgatgtaag ttgcacgatg acgcagacac tgtgcccgca tttgttcccg gctggcttgt
    11941 gcaatgactt cgttctcttc attgacaata tccacccatt cagtacttgc caaacgacgc
    12001 tgttccatta tcaggaaacc ttctttttct ggcgctctta cggcgcattt tgagttgtgg
    12061 gtaaattacg gattaatcgc gacctgcgca atgatacttt gatcattgag tgcgattaca
    12121 ctaagtacgt cattatccag catgccataa ctcgccggat taccgccttt cggaatactc
    12181 accgagccgg ggttgaagtg gaaaatctcc ccccgctgtt ctgccactgg cagatgggta
    12241 tgaccgtaca ccagcacatc gttctggttt aaagcaggta gattttccgg gccaaaaaga
    12301 tgaccatgcg tcaaaaacag acgttgtttt tccagtaata cctgttgcca cggcgcggtt
    12361 atcgggaaat gcagcagcat ttgatccact tcgctgtcgc agttgccgcg cacagcgata
    12421 accttatgtg ccacttcatt aagccgttca gcgactttgg ctggcgcgta accctccggt
    12481 aaagcattac gcgggccgtg attcaacacg tcgccaagga tcaccagcca ctgggcaccg
    12541 ctttgggcaa acaactccag aacacgttcc gtcgccggta acgacccatg aatgtccgat
    12601 gcaaacatca gtttcatcac tcactcctcg tcgaaaaaac gtaagctatg atactggatt
    12661 ctgtcgttgc tatcagccag attgcttcgc cgaaagtgca ataaaacgct ggacagacgc
    12721 tcgctgccac tggaatgtcg cataatccac caggcgttct ggcacctcat cgccatgtag
    12781 taccaggcgg ttaatcatta gcgccagatc agtatcagca atgcaccatt caccaaataa
    12841 attcggctga cccagtacta acaaatgttc tgccatcgca aacagtttct ctgcactggc
    12901 ttttccctcg gccgttagtg gcgctttttt cgcccccgca aagacaacat ccgtcggacg
    12961 ctcttcgcgg atgggcatca gatcgctgcg cagccaggcc tgaatctgtc gtgcacgcgc
    13021 acgattttct aaatcaagcg gataaatacg ttcccaggtc ggtggcgcaa atcgatcttc
    13081 cagatactcc gcaatggcag aagattcact caactcaaaa tcatcgattt gtaataacgg
    13141 cacacggcgt gtctgaccgt aaccttgcca cgtcggctgc aaatgttcac cgctgtcgag
    13201 gtcgatggtc ttgatatgaa acgacaggcc tttttcctgc aacgccaccc aggcggataa
    13261 cacataaggg gagaaaaagt gggcatctga ccaaagcgtg atagcgggtt tactcattat
    13321 gtcctcggca ataaaggggg ctggctatca aactatagag cctttttgtc actgtcacgt
    13381 gacgaatact cacgtaattc gccgcgcttt ctatacttgt ggtgttcaga tgattaaaca
    13441 accggagctg caatgatcga tctctatttc gccccgacac ccaatggtca caaaattacg
    13501 ctgtttctcg aagaagcaga gcttgattat cgcttgatta aggtagacct ggggaaaggc
    13561 ggtcagtttc gtccggaatt tttgcgcatt tcgcccaaca acaaaattcc ggcaattgtt
    13621 gatcattctc ctgccgatgg cggcgaaccg ttaagcctct ttgagtctgg tgccattttg
    13681 ttgtatctgg ctgagaaaac aggactcttt ttgagtcatg aaacgcgtga gcgcgccgcc
    13741 acattacagt ggttattctg gcaggtaggc ggactggggc cgatgcttgg gcaaaatcat
    13801 cattttaatc acgcagcccc ccaaaccatt ccttacgcta ttgaacgtta tcaggttgaa
    13861 actcagcgtc tttaccatgt actgaacaag cggctggaaa actcgccctg gctgggaggc
    13921 gagaactaca gcattgcgga tattgcctgc tggccgtggg ttaatgcctg gactcgccag
    13981 cgaattgacc tcgcaatgta tccggcagtc aagaactggc atgagcggat ccgttcgcgc
    14041 cctgccaccg ggcaggcact gctaaaagca caactcggtg atgagcgttc ggatagttaa
    14101 cagaaacagg ttctcgtgta ttatttcatc ctaagtaaaa caacggagaa cctgcaatgg
    14161 cacaacctgc cgctattatt cgtataaaga accttcgttt acgtacgttt atcggaatta
    14221 aggaagaaga aattaacaac cgtcaggata ttgttatcaa tgtgacgatc cactaccccg
    14281 ccgataaagc gcgcactagc gaagatatca acgatgcgct gaattatcgc accgtaacga
    14341 aaaacattat tcagcatgta gagaataacc gtttctcttt gctggaaaaa ttaactcagg
    14401 atgtgctcga tatcgcacgt gaacatcact gggtgacgta tgctgaagtg gagatcgata
    14461 aactgcacgc gctgcgctac gccgattcgg tatccatgac cttaagctgg cagcgttaac
    14521 cgccatatcg ggaggctgca tgaacatagt gatcaccgga gggacgggat taattggtcg
    14581 ccatttgatt ccgcgtttgc tggagctggg ccatcaaatc acggtagtga cgcgtaaccc
    14641 acagaaagcc agttccgttc tcggccctcg ggtgacgcta tggcaagggc tagccgatca
    14701 aagcaacctc aacggcgttg atgcggtaat caacctggcc ggagaaccga ttgctgataa
    14761 acgctggact cacgagcaaa aagagcgtct ctgccaaagc cgctggaata tcacgcaaaa
    14821 actggtcgat ttgattaatg ccagcgacac gccgccgtcg gtactcattt ccggctcggc
    14881 aacgggctat tatggcgact taggtgaagt ggtggttacc gaagaggaac cgccgcataa
    14941 cgaatttacc cataaactct gcgcccgctg ggaagaaatt gcctgccggg cgcaaagtga
    15001 caaaacgcga gtgtgcctgc tgcgtaccgg tgtagtgctg gcaccggatg gcggtattct
    15061 cggtaaaatg ctgccaccgt ttcgtcttgg cctgggcggg ccgattggtt ccggtcggca
    15121 gtatcttgcc tggattcata tcgatgatat ggtcaacggc attctctggc tgctggataa
    15181 cgagctgcgc gggccattta atatggtttc gccctacccg gtacgcaatg aacaatttgc
    15241 ccatgcgctc ggtcatgcgc tgcatcgccc ggccattttg cgcgtccccg cgaccgccat
    15301 tcgactgtta atgggcgaat cttcagtact ggtattaggt ggacaacgcg cgctgcctaa
    15361 acggctggaa gaagcgggtt ttgcgtttcg ctggtacgat ttagaagagg cgctggcgga
    15421 tgtcgttcgc tgatgtggtt tacagcaaac atccgccagt taactcccgg tgttacagga
    15481 ttagtggctt tgcgcgataa gatcgtctgg tgaaagtcgg gtcaccatca taactaactc
    15541 tctgtctaaa cctctatcca gcatctcctg agcaatacgc agggcttctt cgtgtttgcc
    15601 ctgcattgcg ccttcttcac gtaatctgtc agcaatggtc atcagtttct ccttttcttg
    15661 tggtgcgcgt tccgctatct caccaataaa tgcacgaaaa cgctgggcat cccctgtttg
    15721 taatacgtaa ttaaacaggg cttttagctg tctgtcatta gtgttccctg taactagcag
    15781 cgaaacaatt tggtcgacta atcccaacag atcgcgctga cgaatatgtt tctgaattaa
    15841 ctccaacagc gccattttgc ggtgttgcat aatctcgtca tccggcacca cggtaatatc
    15901 caccaacgga aacgctgatg aatatatttt gtgggctata gcaggctcgg caaattcatc
    15961 aagccagcag agtgaataag gataaggact tctgcaacca tgataaaaca gcatcgggat
    16021 caccaatgga agctctttat agcccgcatc aagatggttt tgcattgccg caatggaata
    16081 acgcatcatg cgaaaagcca ttaattcttc cggcttactt tggtgctcta tcactacata
    16141 aatataaccc actccctcct gcgttttcac agaccacaag aggtcggaat aatattgccg
    16201 caggtcgtca tcaataaaac tgtttggttc cagtttaagc gtcgttaaat cacacagttt
    16261 gcgcagcggc gcgggaagat gaatatcaat aaaatcccgc gcggtgtctg gatggcgtaa
    16321 aaaagattta aataccgcat catgcggcgt ggaagttgtc gatattgtca tggcgttccg
    16381 tcacccctca aatttaagat gacgcgacaa taaccacgat taacacccaa tgcagcaggc
    16441 agtttttatc tttggacagc gaattcagaa gtatttttag cggactgcaa cttttacatt
    16501 tcagcggaag ctgactcgca aattccccat gaaaatactc cggcagcgtt agccaccgga
    16561 gaggaaatta tttcagcgat cccttaagga actgttgcag acgagggctt tgcgggttgc
    16621 caaataactg ctccggcgcg ccctcttctt ctattttccc ctgatggagg aaaatgacat
    16681 gagtagaaac atggcgagca aagcccattt cgtgagtcac taccaccatg gttttcccct
    16741 cttctgccag ttgctgcata atacgcaaca cttcgcctac cagttcagga tcgagcgccg
    16801 aggtaggttc atcaaacagt aaaacttccg gttccatcgc cagcgcccgc gcgatagaaa
    16861 cacgctgttg ctgaccgccg gaaagatgca ccggatattt cccctgcgca cgttcgtcta
    16921 tcccgacttt tgccagatac ttgaccgccc gctcgcgcgc ttcctgcttg ctcaggccca
    16981 acacctgaat cggcgcttcc atgacgtttt ccagcaccgt catatggctc cagagattga
    17041 agtgctggaa taccatcgtc aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga
    17101 ctttgagttg accgtctttg tcgcgcacca gattgatcgt ctggccattg accacgatcg
    17161 acccttcact cggtttttcg aggaagttaa tgcagcgcag aaaggtactt ttccccgatc
    17221 ccgacgatcc gatgatgctt attacatctc cggcattcgc ttgcagtgag acccctttca
    17281 gcacttcatg ttcgccgtag cgtttgtgca aatcgataac gtttaattta ttctcggaca
    17341 tcgtattctc agtgcgttga agaaggtttc acatgctgca accagcgttt ttccgctctg
    17401 cgaaagaggc tgatcaggac ataagagatg attaaataga gcaccgcggc aatgccgaag
    17461 gcggtaaaag gttgatacgt ggcggcgtta atatcgcggg ctattttcag cagatccggc
    17521 accgtggccg taaacgccag cgcagtagag tgcagcatca ggatcacttc gttgctgtat
    17581 gccggtaacg caatacgcag cgcagaaggc aaaataatgc aacgatacat tttaaaagtc
    17641 gagaagccat aggctctggc ggcttcaatt tccccatgcg gaaccgaacg gattgcccca
    17701 gcaaaaatct cggtagtgta agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc
    17761 aggccactgc ggaagaaagc gttaaggaat tcggttccct taacaatctc aagcgtgtac
    17821 atgccggaat agaacaccag caactgaaca tacagcggcg taccgcgaaa aatataggta
    17881 aataaccaga ttggaaactg gatgtattta ttactggaga cacgaccaat cgccagaaac
    17941 agcgccagga ctccgcctat cactaccgac aaaataagca gccacagagt gatcgccaca
    18001 ccagtaaagc gataaccgtc ggtccacagc agcggtttcc agtattcatg taagatttcg
    18061 atcacaggtc agccctcttc acacccacgg agtagcggcg ctcaaggaac agcagcacac
    18121 cattggaaac agtggtgaaa accaggtaaa tcacgccaca gacgatggcg aaatagaacg
    18181 gttcccaggt acttttgcct gccagttggg tggctttgac cacatcttcc aggccgagta
    18241 acgaaaccag tgcggtagat ttgaggatca cctgccagtt gttgccaatg cctggcaggg
    18301 cgtaacgcat catcgccgga aacatgatcc gccgaaacac ttgcccacga gtaaaaccga
    18361 acgccgtcgc cgcctctata tgccctttcg gcactgccat aaacgcgcca cggaacgttt
    18421 cggtaaaata agcaccgtag ataaaaccga gagtaataat accagcgacc atcggatcga
    18481 tatcaatctg cccgacgccc atcgcctccg tcaccgtgtt tagcgcaatc tgcaaaccgt
    18541 agaaaatcag cagcatcaac accaagtccg gcacgccacg aatcagcgtg gtgtaccctt
    18601 cgaaaatcag ccccgaaagc cgattttgcg agagcttacc gccagcgcca attaaaccga
    18661 tgattacagc gagcactaca gaactgatag ccagctccag cgtgacgagc gcaccctgta
    18721 aaataacacc tgaaaaccca tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc
    18781 gcctcttgtc tccctccggt tacccgaagg gagcgggctt tctttcaccg atggcgatta
    18841 accgccataa acatcaaaat cgaagtactt tttcgctaat ttctcgtaag taccgtcagc
    18901 gcgcatttcg gcaaaggctt tgttcagtgc ctcgcgcagt tcgttatctt ctttacgcag
    18961 gcccatgccg gtccctacgc caaacagttt ttcatcttta acagacgggc caccgaattt
    19021 gtaatcttta ccgacgggtt gtttgaggaa accttcactg gcagcgacct catcctggaa
    19081 cgcggcatca atacgtccgg cagtcaggtc agaataaatg ttgtcctgcc cctgatacga
    19141 gacgatttca atgccttttg gtgcccaatg ttcattaccg aacgtctcct gggtggtgcc
    19201 ctgcaatacg cctacccgtt tgcctttcag cgactcgact gtcggctgaa tgtcagaatt
    19261 tttcgccacc accaaacgag aatcggcagc gtacagtttg tcggtgaagg ctatttcttg
    19321 ctgacgtttt tccgtaatgg aaagcgatga catgatggcg tcaatcttct tcgcttttaa
    19381 ggacgggatt aacgcatcca gcggattttc gacaaacgta cattgcgtat tgatgcgttt
    19441 gcataattcc tttgccagat cgatatcgaa gccaaccagt tcgccttgtg aattttttga
    19501 ttcaaatggc gcataggtcg ggtcggtacc gatgcggatg ttttgcggaa tcgcagcaaa
    19561 cgccgcagtt gcgctggaga aggccagaac cagagagagc gatagcacca gttttttcat
    19621 aatgtcctca actgacagtc tttttatagg gaatatttac aggtttgtta cacttatcgt
    19681 gccataaaat tgaccatcaa ggcaaaatat cccgccccgt tagacgtatt atttttcatt
    19741 aaaaatgctt aagtatgcat ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc
    19801 atgatgatca ttccatcagg tacagcttcc cagcgacgta tcagtcaccg tagacattaa
    19861 agtcgaaata ctttttcgcc atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg
    19921 ccttattgaa ggcagccgtc agttcagcat catctttacg tagccctaca ccggtgccat
    19981 caccgaagta ttttttgtct tttactgatg agccagcaaa ggcgaaatct ttaccagcag
    20041 gttgcttgag gaatccttcg ctggcagcaa cttcatcttg taacgcagca tccagacgtc
    20101 ctgcagccag atcggaatag accaaatcct ggttggcata ggccaccaca tccacgcctt
    20161 tgctacgcca ggtctcgtta gcgtaagctt cctgggttga tccctgcagc acaccaacat
    20221 gtttaccttt cagtgaatcc agcgttggct gaatcggtga acctttggca gcaatcaaac
    20281 gagaatctgc ggcgtacagc ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa
    20341 tggaaagcga cgaaataatg gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt
    20401 caaagtcact ggcaacccag gtacatttca cctgcatccg tttgcacatc tcgttaccga
    20461 gatcgatatc aaagccaaca aaatcacctt tagcatcttt cgacgagaac ggcgcatagg
    20521 tggtatcggt tccgatacgt accgtctccg gtagcgccgc atagctggaa gccgctgcgg
    20581 agagaccgac taacaaagac agagcgagaa tcgacttctt catacataac cctcaagtga
    20641 aaatggcttt tttatgttgt gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct
    20701 tatgccatct tgacgacaac agtaacattc aacgttaaat atgttaataa gacgttgcat
    20761 tattgtcctg aagttgaaga tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat
    20821 ggcagaaaca aaatgtcgag gatttgatcg cggttgcaaa attgccctga aacagggcaa
    20881 cagcggagtt atgcgccctg ccagcgggca aagagatctt caggaagggt tatcgcaaac
    20941 tggtcaagaa cacgattaac cgtctgattt atcacatcat caagggattg cgggcgatga
    21001 taaaacgccg gaacgggagg cataatcacc gcaccgattt ctgccgcctg agtcattaaa
    21061 cgcagatggc ctaagtgcaa tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct
    21121 ttcagcacca catctgccgc acgggtcagt aagccatcag tatagctatg gacaatgccg
    21181 gaaagggttt tgattgaaca gggtaaaatc accatcccta gcgtctggaa agaaccggaa
    21241 gagatgctgg cggcaatatc gcgcgcatcg tgcgtgacat cggctaatgc ctgcacttcg
    21301 cgcagagaaa aatccgtttc gagggataag gtctggcgcg ctgcctggct catcaccaga
    21361 tgcgtttcga tatctgtgac atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg
    21421 ccgctggcgc cgctgatgcc tacaatgagt cgtttcataa atagttgccc tgtcagactt
    21481 gcaggcagac tttgcaggat ttcggcggga gttgcaagtc agggtgccag accggcaccc
    21541 tcagcgaagg catcatcctt cgttatgcat ttcgagattt tccacttcgt tctgacgttg
    21601 cactgctttg gcgtcatcat tacgtaacgt atcgaggaaa tcgaggtagc cctgatcaac
    21661 atctttggtg acgtagacgc cgttgaacac cgagcattca aactgctgga tatccggatt
    21721 ttcagcgcga acggcgtcga tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc
    21781 gatgatctgg cgaatttcat caacttcgcg accgtgagcg atcagttccg tggcgctcgg
    21841 catatcaata ccataaacgt tcgggaagcg aatttccggt gccgcagaag cgaggtacac
    21901 tttcttcgct ccggcttcgc gtgccatctc gataatctgc tcagaagtgg tgccacggac
    21961 gatggagtcg tcgaccagca ggacgttttt atcgcggaac tcggcgcggt tggcattcag
    22021 tttacggcgc acggacttac gacgcagctg ctggcccggc atgataaagg tgcggccaac
    22081 atagcggttt ttaacgaagc cctggcggta cggtttgccc agaatacgag caatttccag
    22141 cgcgatatca cacgaggttt ctgggatcgg gatcaccacg tcgatatcca gatcttccca
    22201 ttcgcgggca attttctcgc ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga
    22261 aattttgtcg atgaacgagt ccgggcgagc aaagtataca tactcaaaca ggcacggatt
    22321 gctgaccgga ttgtcagcac attgacgggt aaacaactgc ccttcttcag tgatgtaaat
    22381 cgcttcgccc ggcgcgacgt cacgcaggaa atcaaagccc agcgtatcga gcgctacgct
    22441 ttcggaagcg accatatatt ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag
    22501 cggacgaatc ccgtttggat cgcggaaagc aaccataccg tggccgataa tcatcgccac
    22561 acaggcatat gcgccgcgga ttaagcggtt tgtggcagca atggcagcga aaatattgtc
    22621 ggcttccagc gggtagtggc ggaagttgtc cagctcgcta gcgaagatat taagcagaat
    22681 ttccgagtcg gaagtggtgt tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa
    22741 ctcgtgagcg ttggtcagat tgccgttgtg ggcaagcgta atgccatacg gagagttaac
    22801 gtaaaacggc tgcgcttcag aggcgctgga gctgccagcc gtagggtaac gcacatgacc
    22861 aatgcccata ttgccctgca aacgctgcat atggcgagct tcaaatacat cgctcaccag
    22921 cccgttcgct ttacgcaaac ggaagcagtt attggcatct atggtgatga tgccggcggc
    22981 atcctgaccg cgatgctgaa gcaccgttaa ggcatcataa atcgactggt taaccggcat
    23041 aacaccggcg ataccgacaa taccgcacat acgtcttttt cctcgttaag ccacatctca
    23101 gagcacttac gctctgggca agaaacttga cgagctttgc agataatcaa aaaaccatct
    23161 gatgataaaa ctgaactgcg ggatcagctg tgatttgctc cagtcttcgc ttttcgacac
    23221 cccggtaaag gagtcgagaa agaagagaat ggcggcaaca atcaacacac cgcgcaacgc
    23281 accgaaacag acgcccagca cccgatcggt gcctgacaac cccgttttct ccaccaactg
    23341 gcctatcacg aagttcacga tagcaccaac gatcagggta gcgataaaca gtaccgcgat
    23401 ggcaattcca tttcgaacca gttcgtcttc aaagcccgta aaccagactg acaggtaagt
    23461 gtagtaatga ctggcaacaa agaaagcaca accccatgtc accagcgata acgcttcacg
    23521 aacaaagccg cggatcaggc taaccagaga ggaaaaagca atcaccgcga ttatggcgta
    23581 atcaatccag accatatgtg tcccacgatt ttacgccctg tcatcctgtt cggggcgcat
    23641 tctaacagaa aaagaaaacg tttgcgtagg gatttccttc ccgcgcatca ataaaaatgg
    23701 cgctgaaaaa atattcaacg ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt
    23761 gtcggatgcg gcgtaaacgc cttatccgac ctacggttct aacactgccc aggcctgata
    23821 agacgcgcca gcgtcgcatc aggcaagact gtattaattc ggcgtatagc ccattaccac
    23881 gccacttaag ccagaaagtt gcttcaactc acccagcgaa cctttcagct tatctttcga
    23941 ggcatccggc ccaaccagaa tacgggtaat tttaccctgc actggcgtgg atggcgacgt
    24001 ataaacccga taaccggcac cgcgcagctt accgacaatc tcattcactt tatcggcatt
    24061 tttcagcgca cccagttgca caacataggc tttacccgtt ggcgctgctt tttcttccac
    24121 gaccggcttc ggttctggct tcggcgcagg tggtgcttca accttcggtt taggcggctc
    24181 caccggtttc ggcttcggtg gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt
    24241 ggctgcaata gtggtcggat cgagcgacgg tgctgcggca tcacctgccc gcacctcttc
    24301 cgctgcgcct tccggcggct gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg
    24361 ctcatcgcga tcgcccgctt tcggcaccag cgggatagcc gcgaactcat cctgataatg
    24421 ttttttctgc ccgtccagca gccctggaag tacaatcacc cccagcgcca ccagcacgat
    24481 cgtgcccact aaccgattct gaaacttact tgccaccgct tctcctcgcg tcaatcactt
    24541 ccatgacatg tgcgacagtg tggaaagaac cacacaccag cacggtgtct tccgctttag
    24601 cgtccgccat tgcggcatcc catgcctgcg caacgctatc aaatgatttg ccgttaccca
    24661 aatgctcaag cagttgttct gccgtggcac cgcgcggccc ttccagtggc gcacaatacc
    24721 agtcatcaac cacgcttttc aaccaggcca gagttccggc aatatcttta tcatgtagca
    24781 taccgataac cgccagcacg cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat
    24841 attccgccgc atgtggatta tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa
    24901 tctggaaacg tcccggcaaa attgcgctgg caatcccgtc gcgaatggca ttttcactga
    24961 cttccagccc gctggcacgc agtgccgcca gcgccgttgc ggcattcggt tgcgggacaa
    25021 gcggcaacgg cagattttcc agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg
    25081 tgacggaata gttccactca acgccccgac gttgtaacag tgcacctttt tcctgtgcca
    25141 catcagcaat ggtagaaggc atttccggct caccgacaat tgccggtttt tcgctgcgga
    25201 agatgcctgc tttctcgcga ccaatacttt cgcgatctgg acccagccag tcggtatgat
    25261 ccagcgcaat actggttact accgcgacat cggcatcgac aatattggtt gcgtccagac
    25321 gaccgcccag ccctacttcc agaatcacca cgtcaagttg tgcctgcttg aacagccaca
    25381 acgccgacag cgtaccgtac tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa
    25441 tctccgcaaa agaggcggtg tgggccgatt ccggcaattc ctggccctgc acacgtacgc
    25501 gctcggtata acgcaccaga tgcggtgaac tgtagacgcc cactttgtac cctgccgcca
    25561 tcagaatcga ctccagcgta cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa
    25621 acacaaatgg cgctggtttc agaacgccaa gacgcgccgc gacctggctc acgcgctcaa
    25681 ggccgagatc gatagtttta ctgtgcaggt tttccagata agaaagccac gaagccagag
    25741 gcgacgcggc ttgaggagtg cgtttgataa tcatggtatc cgctgattcg ttacggtgag
    25801 aatagcaaaa gggcagagcc agtggccctg cccttatcag ttatcaggcc tcaggttcct
    25861 gatccggtac cgggggtacc actacgcctt cacgcggcgc ttcaggattc ggcgctggca
    25921 gattcatcaa cttcgccaga atgctcgcca gtttcaggcg catttccgga cgacggacga
    25981 tcatgtcgat ggcgcctttc tcgatcagga attcactgcg ctggaatcct ggcggcagtt
    26041 tttcgcgaac ggtctgttcg ataacacgcg gaccggcaaa gccaattaac gctttcggtt
    26101 cagcgatgtt gagatcgccc agcatggcga aacttgcaga aacaccgccc atcgtcgggt
    26161 cggtcagcac ggagatgtac ggcaagccgc gctcctgcat ttttgccagt gccgcagagg
    26221 ttttcgccat ctgcatcagc gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg
    26281 agaagcagat cagcgggcag ttatcttcca gcgcctgctc aacggcacgc acgaaacgtg
    26341 cacccacaac agaccccatt gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa
    26401 ccggcattcc atacagagtg cctttcatca ccaccagcgc atctttttcg ccagtttctt
    26461 tctgcgcaga tgccagacgg tctttatact tcttggagtc acgaaacttc agcacatctt
    26521 tcggctcaag ctcgctaccc agctccacaa ggcttccttc atctaacagg ctatgcaggc
    26581 gattacgcgc tgtcatacgc atgtgatggt cacacttcgg acagacctca agattacgtt
    26641 ccagctcagc gcggtataaa acctgaccgc agctatcaca cttagtccac accccttcag
    26701 gaatgctcgc cttgcgggtg ggagtaatgt tgcttttaat tcgttcaatc cagctcatta
    26761 gggacctttc tgtctgaacc tggttcgatg ccagttttat ctttggggac gcataatgcc
    26821 atttttgccc ccaacagacc atgaatgttg cacattaaaa cataacagcc cgaaactttg
    26881 gataaaaaag tggtcgaacc gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg
    26941 tttgtgacga ataaattcga aaaccccagg caatattgaa acaaaaataa tgccaacaat
    27001 catcagctta aggttgctct gaatgaaggg gagcgtgccg aagaaataac ccgcgtaggt
    27061 aaaaatcagt acccacaaca gtgcgccgat cacgttataa gcggcgaaat gacggtacga
    27121 catgtggccc attcccgcaa caaacggcgc gaacgttctg acgatcggta caaaacgggc
    27181 gagaataatc gttttgccgc catgtctctc gtaaaactga tgagtcttat caagataact
    27241 gcgacgaaag attttcgaat ccgggttact gaacagtttg tcaccaaata accgtccaat
    27301 ggtatagttc actgcatcac cgacaatagc ggcaatcaac ataagcacca ccatcatatg
    27361 gacattgaga tcgttagttt ccagcgacgc caatgcacca gcaacaaaca gcagcgaatc
    27421 accaggcagg aatggcgtta ccaccagccc cgtttcgcag aacagaatca aaaacagaat
    27481 ggcataaacc cagacgccgt actccgcgac cagctctgcc agatgcacgt caatgtgcag
    27541 gataaaatcg atgaggaaat aaatcaggtc catatttgcc tatgccttgt actcgtcatc
    27601 tttcaggctg taactacgtt ggctacagcc tgaaatgttc cgagcattat tctcgttagt
    27661 ccgccagaaa tagcgggccc attggcggtt ttggaagatc ataccggtca gggtaatcca
    27721 ccgcgaccag atacagccct tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg
    27781 ccgccagcaa ctctgctatc cagctctccg gctggttgtg ggcaccgact tccatcaggc
    27841 tgccgacaat attcctgacc atatgatgta caaaggcatt cgctttgata tctaccacca
    27901 cataaggacc gtgacgcgtg acattaatat gcataacgtt acgccacggg gttcgggact
    27961 ggcactgcac cgcacggaac gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc
    28021 gatgcatccg ttcagcgtcc agcggttcgt aaaaatgggt taccccttta ctcagtaccg
    28081 ccgggcgcag ccgatgattg tagatgatgt agcgataacg gcgagccgtg gcgctaaatc
    28141 gggcatgaaa atcatcaggt acagctttaa cccaacgcac ggcgatgtca ccaggtaaat
    28201 tcgcatttac gcccaaggtc cacgccgcgt ctttgcgctg cgcggttgtt tcgaaatgca
    28261 ccacttgccc ggtaccgtgt acccctgcgt cagtacgccc ggcgcagaag acggtgatgg
    28321 gttcgttcgc cacctgggag agcgcctttt ccagcttctc ctgcacactg cggacttcat
    28381 tctgccgttg ccagccgtaa tacttactgc cgtcgtactc aatgcccagc gcaattttat
    28441 aaactggcag ttgttgctgg tcggacatta gtacagatac tcctgcacca gtttctcggc
    28501 gatttttact gccatcagcg cgccgccaaa gcgaacgtta tcggccaccg accagaactg
    28561 gacttgctcc ggcataccgt agtcattacg cacgcagcca acagaaagat gcggcgtacc
    28621 cgaagcatcc cctacctgag tcgggaattc gttctcttca gagagcacaa tatcttcgcc
    28681 ctgagcaaac gcatcacgcg cttcttctgc cgccagcgga cgcagtgctt caaagttgac
    28741 catctgggca tgaccgtaga ataccggtgc ctggacgacg cttgccgaaa tcatcagccc
    28801 ttcgtcctgc aggattttgc gtacttcgtc aacgatacga cgttcttcac gcacgctacc
    28861 ttcgctatcc ggcaacaacg gcagcatatt gaacgccagt tgacgaccga agaaatcttc
    28921 ttcgtcaatc ggaatgccgt tgagcaattt cgcactctgc cccgctaacg catcgaccgc
    28981 ttttttgccc tgggcggagg ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc
    29041 ctgatcgatt aacggtttca gtgccgccag cagctggctg gtcagactgt ctggtacggc
    29101 gatgacattc cggttgcggt aatcggtcag tacaaacggg tttacttccg gcaccaccag
    29161 cggtacgtcg ggttcgagag caaacagtcc gctgctgtcg atcaccaggc aacctgagtt
    29221 ggtcgcttct tcaacccagg cagcggtagc ttctttgcct gcgacaaaaa atgccagctg
    29281 cgcctgcgtc cagtcgaatt cagcggcatc ctgcacggtg attgtcttac caccaaagcg
    29341 cagttgttcg cctgcgcttt cgttacgtgc cagtgcataa atttccccaa ccgggaactg
    29401 acgttcagcc agcgtttcaa gcagggcttc gcccacagcg ccagttgcgc ccaggacggc
    29461 aatgttccag ccttcagaca tggtggttta ctccagaaat agcaaagctc cctgccaatg
    29521 ttacagcaga gagcatgaag aagagattaa cgtgccggat gatgaacggc gttaaaaccc
    29581 agtttacaca gcaatgatgc cgcactggcg tcatcacaaa ttacatacag agatgaccat
    29641 tcacggcgct caagatagtt tttgcgcagt ttatcgaact cacccggtat cccggcgact
    29701 ttacgcagcg gtgcgtcatc gcggcgcaca tcatacacca aatgcaccag ccttttcagc
    29761 gtcggttgat cgagcgggcc atgcagcgta atgcgaccaa actctggcgc gggcagtaat
    29821 gtatccagcg caacgtgctg ttcatgccca ataaacttgc tataggcttc aaacacttgc
    29881 gtagtaccgc gtgctttacc ttccagggta taacctgcga tatgcggcgt gccgatatcc
    29941 acttttgtca gcaactccac gttaagttcc ggttcgcctt cccagacatc cagcactacg
    30001 cttaacttct ggccttcatt caggcaggtc agcaacgcag tattatcgac gactgcgcca
    30061 cggcaggcgt taatcagaat cgctcccggc ttcaggctac ggatcagttt ttcatccgcc
    30121 agatgcagcg ttttgtacgg cccatctttg aagagtggcg tatggaaagt cagaatatcc
    30181 gcatgctgaa ctaattcatc cagcgagcgg aaatcaccct catccccacg gtcagcgcga
    30241 ggcggatcgc aaagcaatgt tttaatccct aacgcttcca gtcgcgcctg caatcgacgt
    30301 ccaacgttac caacgcccac gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt
    30361 tcggcaagca tcagtagtga ggagaaaaca tattccacca ccgcaatcgc attacagcca
    30421 ggtgcagcgg aaaaaccaat tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc
    30481 cccgcagtgg cagtgccaac aaatttaatg ggttttcctg ccagcaaaga ttcattcact
    30541 ttcgtgaccg aacgcaccat cagcgcatcc gcgtctgcca gttgagcaac ggggattgga
    30601 cgcccgggaa ccgcggtcac ctcacccaaa cggctaaata agtcgcgggc ataaggcata
    30661 ttttcatcaa caaggatttt cacgtttgtg ttacctgtat gagacgagag ttaaccggac
    30721 aagtgtgcca taatctcgcg gccaggcata cttgcgaaga tttcaggtat aaggatacgt
    30781 aatgatacaa cctatttccg gccctcctcc tgggcaacca ccaggtcagg gagataacct
    30841 gccgtctggc gcgggcaatc agcctttatc cagtcagcaa cgtacttcgc tggaaagctt
    30901 aatgacgaaa gtgacctcac tgacgcaaca acaaagagca gaactgtggg cgggtatcag
    30961 gcacgatatt ggtctgtcgg gagattcacc gctgctttcg cgtcacttcc ctgccgctga
    31021 gcataatctg gcgcaacgtc tgctggccgc gcaaaaaagc cattctgccc gccagctttt
    31081 agcgcaatta ggggagtatt tacgtctggg gaataatcgt caggcggtca cggattatat
    31141 ccgtcataac tttggtcaga cgccgctgaa tcagctctca ccggagcaat taaaaaccat
    31201 tctcaccctg ttgcaggaag ggaagatggt tattccgcaa ccacagcagc gcgaggcgac
    31261 cgaccgtcct ttattaccgg cggagcacaa tgcgctaaaa cagctggtga ccaaacttgc
    31321 ggcggcaacg ggggaaccca gcaaacagat ctggcaatcg atgctggaac tttccggggt
    31381 gaaagatggc gagttaattc cagcgaaact gtttaaccat ctggtgacct ggttgcaggc
    31441 gcgccagacg ttaagccagc aaaatacgcc gacgctggaa tcactacaga tggcgctaaa
    31501 acaaccttta gatgccagtg aactggcggc gttatcggca tatatccagc aaaaatatgg
    31561 cctttctgcg caatcatcgc tttcttctgc ccaggccgag gatattctta atcagcttta
    31621 tcaacggcgg gttaaaggga ttgatccgcg tgatatgcaa ccgctgctta atccttttcc
    31681 accgatgatg gacacgttgc aaaatatggc aacgcgtccc gcgctgtgga tactgttagt
    31741 cgcgattatc ctgatgctgg tctggctggt tcgttagccc cgacgaaatg acagtatcgt
    31801 gacaataatt cccagcaccg cagagatcgc cccggcaaga aataccgaag agtaaccaaa
    31861 cgtggtcgcc agcattcccg ccagcggccc ggagacgccg agggcgatat cctgaaacgc
    31921 ggcgtaaccg cccagtgcgg tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac
    31981 gcccagcgca ggaaagataa gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc
    32041 gacccacgca cctggggctt gccagagcag caacaagccc accgtttcta caagcagaga
    32101 gacaatcgcc actttcacgc cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat
    32161 cacgacaaat gcgccgccaa acgcggtaag agtaaagccc gccatcgccc atcctttgct
    32221 ggcaaagtag agcgaaacga aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag
    32281 ccctaaccct ggtttccaga taaacccgac aacgctccac agcgatggac gttctcccgc
    32341 cagggccggt actttgcgca ctgtgccgtt acaggcccac gccagtaagg gtaataccat
    32401 tgtggtgatc gccagtgcgg caaaaccgta atggctatga atcaacaggc caagcggagc
    32461 accaacagcg agggcaccgt aaatcgccat tccattccat gacatcactt tgccagagtg
    32521 ttttggccct acgatgccta acccccaggt cagagcgcct gtcagtaact ggctttcacc
    32581 aaacccaaga atcaaacgcc cgatgaccaa cagggcaaat ttgaacggtg cggagacagg
    32641 caaaatcgcc gccagcagca acgcgccgcc agccagacca caagctaaca tcccctgaag
    32701 cgccgaacgt tttgcaccat attgatcggc cagtcgcccg gcatagccac gcgtcagcac
    32761 cgtagccaga aactgaatcc cgacggcaat cccgaccatg gtattgccat agcccagatc
    32821 atgatgaaca aacagcggga taaccggcaa cggcagccct acggtcatgt aggtgagaaa
    32881 aaccgcaaaa gcgatgcgga agagcgaaaa attggcagaa gatcgtgttt cggtttggct
    32941 tacagcagtc atgcattact ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc
    33001 tctctggttt cagggttaca gtgcgttggc aggatttaac gcgtacgtct tttcagaagg
    33061 aaatcgacaa agcgggaagt ttgcctggaa ctggcggcga ttgtcaatga tgtgaaaaag
    33121 ggaaccatca ggttcccttt tgcgttagtg ccggaggccg cattggcgcg taacgtcgga
    33181 tgcgacgctg gcgcgtctta tccgacctac tccgactact ccgacctact gcgaattaat
    33241 ctttcagctt gcgcattacc agcgtggcgt tggtgccgcc gaagccgaag ctgttagaca
    33301 taacggtagt cagttcgcga tcggtcgttt cggtcacgat gttcagaccc gcagcctgct
    33361 cgtccagctc ttcaatgttg atgctcgggg cgataaagcc gtgttccagc atcagcagag
    33421 agtagatagc ttcctgtacg ccagcagcgc ccagagagtg accggtcatg gctttggttg
    33481 cagaaatcgc cgggctctta tcgccgaaca cttcacggat agctgccagt tctttcacgt
    33541 cgccaaccgg agtcgaagta ccatgagagt tcaggtaatc gattggggta tcaacgccgt
    33601 gcatcgccat cttcatgcag cgtactgcgc cttcgccaga cggagcaacc atgtctgcac
    33661 catcagaggt tgcgccgtag ccaacgattt cagcatagat gtgagcacca cgcgccagcg
    33721 cgtgttccag ctcttcaacc actaccatac cgccgccgcc agcgataacg aaaccgtcac
    33781 ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc gttgtattta gtagacagcg
    33841 cacccatcgc gtcgaattcg caggccattt cccagcacag ctcttcgccg ccgccagcaa
    33901 acacgatgtc ctgtttgccc agttggatct gctctactgc gttaccgata cagtgtgcgg
    33961 aagtcgcaca cgcggagctg atggagtagt taacgccatg aattttaaac ggcgtggcga
    34021 ggcaggcaga aacgccggat gccatcgctt tggtgaccac atacgggcca accgctttca
    34081 ggccgcgcgg gccgcgcatt gcgtcagcgc tgaacacctg gaaacgcggg gaaccgccgc
    34141 cagaacctgc aatcaggcca acgcgcgggt tattctggta agcttccgga gaaaggcccg
    34201 catctgcgat tgcctgctcc atagaaagga atgcataaat ggatgcgtcg ctcataaagc
    34261 gcacaacttt gcggtcaatg aggccagtgg tatccagttt tacgttgccc cagacgtggc
    34321 tacgcatgcc ggaatccttc agctcctgag agaaagtgat ccctgaacgt ccttcacgca
    34381 gagatgccag gacttcctgc tggttattac cgatgctgga aacaatgccc aggccagtaa
    34441 tcactgcacg tttcattcaa tacctctgta agtcgcacat agagtaagtt tcgaatgcac
    34501 aatagcgtac acttgtacgc cgaacaagtc cgatcagcca tttaatagag aaatttgcgc
    34561 agccttacac acatcgctaa gatcgagcca ccgcctgtaa gacgagtaac ttacgtgaaa
    34621 cactactcca tacaacctgc caacctcgaa tttaatgctg agggtacacc tgtttcccga
    34681 gattttgacg atgtctattt ttccaacgat aacgggctgg aagagacgcg ttatgttttt
    34741 ctgggaggca accaattaga ggtacgcttt cctgagcatc cacatcctct gtttgtggta
    34801 gcagagagcg gcttcggcac cggattaaac ttcctgacgc tatggcaggc atttgatcag
    34861 tttcgcgaag cgcatccgca agcgcaatta caacgcttac atttcattag ttttgagaaa
    34921 tttcccctca cccgtgcgga tttagcctta gcgcatcaac actggccgga actggctccg
    34981 tgggcagaac aacttcaggc gcagtggcca atgcccttgc ccggttgcca tcgtttattg
    35041 ctcgatgaag gccgcgtgac gctggattta tggtttggcg atattaacga actgaccagc
    35101 caactggacg attcgctaaa tcaaaaagta gatgcctggt ttctggacgg ctttgcgcca
    35161 gcgaaaaacc cggatatgtg gacgcaaaat ctgtttaacg ccatggcaag gttggcgcgt
    35221 ccgggcggca cgctggcgac atttacgtct gccggttttg tccgccgcgg tttgcaggac
    35281 gccggattca cgatgcaaaa acgtaagggc tttgggcgca aacgggaaat gctttgcggg
    35341 gtgatggaac agacattacc gctcccctgc tccgcgccgt ggtttaaccg cacgggcagc
    35401 agcaaacggg aagcggcgat tatcggcggt ggtattgcca gcgcgttgtt gtcgctggcg
    35461 ctattacggc gcggctggca ggtaacgctt tattgcgcgg atgaggcccc cgcactgggt
    35521 gcttccggca atcgccaggg ggcgctgtat ccgttattaa gcaaacacga tgaggcgcta
    35581 aaccgctttt tctctaatgc gtttactttt gctcgtcggt tttacgacca attacccgtt
    35641 aaatttgatc atgactggtg cggcgtcacg cagttaggct gggatgagaa aagccagcat
    35701 aaaatcgcac agatgttgtc aatggattta cccgcagaac tggctgtagc cgttgaggca
    35761 aatgcggttg aacaaattac gggcgttgcg acaaattgca gcggcattac ttatccgcaa
    35821 ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc tggaactggc gcaacagcag
    35881 ggtttgcaga tttattatca atatcagtta cagaatttat cccgtaagga tgactgttgg
    35941 ttgttgaatt ttgcaggaga tcagcaagca acacacagcg tagtggtact ggcgaacggg
    36001 catcaaatca gccgattcag ccaaacgtcg actctcccgg tgtattcggt tgccgggcag
    36061 gtcagccata ttccgacaac gccggaactg gcagagctga agcaggtgct gtgctatgac
    36121 ggttatctca cgccacaaaa tccggcgaat caacatcatt gtattggtgc cagttatcat
    36181 cgcggcagcg aagatacggc gtacagtgag gacgatcagc agcagaatcg ccagcggttg
    36241 attgattgtt tcccgcaggc acagtgggca aaagaggttg atgtcagtga taaagaggcg
    36301 cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa tggtaggcaa tgttcccgat
    36361 tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc agaaagatga ggcggtaagc
    36421 gcgccggttt ttgacgatct ctttatgttt gcggctttag gttctcgcgg tttgtgttct
    36481 gccccgctgt gtgccgagat tctggcggcg cagatgagcg acgaaccgat tccgatggat
    36541 gccagtacgc tggcggcgtt aaacccgaat cggttatggg tgcggaaatt gttgaagggt
    36601 aaagcggtta aggcggggta atctgctctc gcagtgtttg ccggatgcgg cgtgaacgcc
    36661 ttatccgacc tacgtgtgac
//
LOCUS       DAZFXT010000040        36024 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.040, whole genome shotgun sequence.
ACCESSION   DAZFXT010000040 DAZFXT010000000
VERSION     DAZFXT010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.040"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(88..369)
                     /locus_tag="ACHKW0_002064"
     CDS             complement(88..369)
                     /locus_tag="ACHKW0_002064"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7474898.1"
                     /translation="MSQTILFVCATGIATSTAVTEKVMEYCKEHGLNVNYSQTNVASL
                     PGNTDGVALVVSTTKVPYELDVPVVNGLPIITGIGEEKVLAQIVSILKN"
     gene            complement(366..839)
                     /locus_tag="ACHKW0_002065"
     CDS             complement(366..839)
                     /locus_tag="ACHKW0_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7474899.1"
                     /translation="MQGIHFQENYIQRLPAGLSVEQIIRQLAQPLVAAELVVPDFADH
                     VLKREATYPTGLPTEPPCVAIPHTDHKHVRHNAIAVGILPEPVEFADMGGDPDPVPVR
                     VIFLLALSESNKQLNALGWIMEMIQDTPFMQALLAMETTEIHTVILNKMKERGEI"
     gene            complement(864..1610)
                     /locus_tag="ACHKW0_002066"
     CDS             complement(864..1610)
                     /locus_tag="ACHKW0_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HFO7474900.1"
                     /translation="MKSLSKSSQIPLYQQVVEWIRESIYTGDLVEDDRIPSEYQIMDM
                     LEVSRGTVKKAVAQLVKEGVLIQVQGKGTFVKKENVAYPLGEGLLSFAESLESQKIHF
                     TTEVITSRIESANRYVAEKLRITPGQDILYLERLRSIGDEKAMLIENRINIELCPGIA
                     EIDFNQHNLFPTIESLSQRKIRYSESRYAARLIGNERGHFLDISEDAPVLHLEQLVFF
                     SRGLPIEFGNVWLKGNKYYLGTVLQRREVS"
     gene            complement(1809..2210)
                     /gene="nikR"
                     /locus_tag="ACHKW0_002067"
     CDS             complement(1809..2210)
                     /gene="nikR"
                     /locus_tag="ACHKW0_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HFO7474901.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(2216..3022)
                     /gene="nikE"
                     /locus_tag="ACHKW0_002068"
     CDS             complement(2216..3022)
                     /gene="nikE"
                     /locus_tag="ACHKW0_002068"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HFO7474902.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(3019..3783)
                     /gene="nikD"
                     /locus_tag="ACHKW0_002069"
     CDS             complement(3019..3783)
                     /gene="nikD"
                     /locus_tag="ACHKW0_002069"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HFO7474903.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(3783..4616)
                     /gene="nikC"
                     /locus_tag="ACHKW0_002070"
     CDS             complement(3783..4616)
                     /gene="nikC"
                     /locus_tag="ACHKW0_002070"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015099 - nickel cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HFO7474904.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(4613..5557)
                     /gene="nikB"
                     /locus_tag="ACHKW0_002071"
     CDS             complement(4613..5557)
                     /gene="nikB"
                     /locus_tag="ACHKW0_002071"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HFO7474905.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(5557..7131)
                     /gene="nikA"
                     /locus_tag="ACHKW0_002072"
     CDS             complement(5557..7131)
                     /gene="nikA"
                     /locus_tag="ACHKW0_002072"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015413 - ABC-type nickel transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0015675 - nickel cation transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7474906.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKALLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(7242..7829)
                     /gene="acpT"
                     /locus_tag="ACHKW0_002073"
     CDS             complement(7242..7829)
                     /gene="acpT"
                     /locus_tag="ACHKW0_002073"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HFO7474907.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(7884..8933)
                     /locus_tag="ACHKW0_002074"
     CDS             complement(7884..8933)
                     /locus_tag="ACHKW0_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7474908.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            9065..10282
                     /locus_tag="ACHKW0_002075"
     CDS             9065..10282
                     /locus_tag="ACHKW0_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7474909.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     SVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(10286..10843)
                     /gene="dcrB"
                     /locus_tag="ACHKW0_002076"
     CDS             complement(10286..10843)
                     /gene="dcrB"
                     /locus_tag="ACHKW0_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HFO7474910.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(10916..11581)
                     /locus_tag="ACHKW0_002077"
     CDS             complement(10916..11581)
                     /locus_tag="ACHKW0_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HFO7474911.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            11802..12047
                     /gene="tusA"
                     /locus_tag="ACHKW0_002078"
     CDS             11802..12047
                     /gene="tusA"
                     /locus_tag="ACHKW0_002078"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HFO7474912.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(12149..14347)
                     /gene="zntA"
                     /locus_tag="ACHKW0_002079"
     CDS             complement(12149..14347)
                     /gene="zntA"
                     /locus_tag="ACHKW0_002079"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HFO7474913.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(14421..15047)
                     /locus_tag="ACHKW0_002080"
     CDS             complement(14421..15047)
                     /locus_tag="ACHKW0_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HFO7474914.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            15188..15547
                     /locus_tag="ACHKW0_002081"
     CDS             15188..15547
                     /locus_tag="ACHKW0_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HFO7474915.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(15550..15819)
                     /locus_tag="ACHKW0_002082"
     CDS             complement(15550..15819)
                     /locus_tag="ACHKW0_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HFO7474916.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(15809..16405)
                     /gene="rsmD"
                     /locus_tag="ACHKW0_002083"
     CDS             complement(15809..16405)
                     /gene="rsmD"
                     /locus_tag="ACHKW0_002083"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HFO7474917.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            16555..18048
                     /gene="ftsY"
                     /locus_tag="ACHKW0_002084"
     CDS             16555..18048
                     /gene="ftsY"
                     /locus_tag="ACHKW0_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HFO7474918.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPVEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLF
                     RGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMG
                     EILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFRA
                     AAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSH
                     LMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDG
                     TAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            18051..18719
                     /gene="ftsE"
                     /locus_tag="ACHKW0_002085"
     CDS             18051..18719
                     /gene="ftsE"
                     /locus_tag="ACHKW0_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HFO7474919.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            18712..19770
                     /gene="ftsX"
                     /locus_tag="ACHKW0_002086"
     CDS             18712..19770
                     /gene="ftsX"
                     /locus_tag="ACHKW0_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HFO7474920.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            20015..20869
                     /gene="rpoH"
                     /locus_tag="ACHKW0_002087"
     CDS             20015..20869
                     /gene="rpoH"
                     /locus_tag="ACHKW0_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HFO7474921.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            21140..22243
                     /gene="livJ"
                     /locus_tag="ACHKW0_002088"
     CDS             21140..22243
                     /gene="livJ"
                     /locus_tag="ACHKW0_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HFO7474922.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(22442..22825)
                     /gene="panM"
                     /locus_tag="ACHKW0_002089"
     CDS             complement(22442..22825)
                     /gene="panM"
                     /locus_tag="ACHKW0_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0031638 - zymogen activation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HFO7474923.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            23249..24358
                     /gene="livK"
                     /locus_tag="ACHKW0_002090"
     CDS             23249..24358
                     /gene="livK"
                     /locus_tag="ACHKW0_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HFO7474924.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            24406..25332
                     /gene="livH"
                     /locus_tag="ACHKW0_002091"
     CDS             24406..25332
                     /gene="livH"
                     /locus_tag="ACHKW0_002091"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HFO7474925.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            25329..26606
                     /gene="livM"
                     /locus_tag="ACHKW0_002092"
     CDS             25329..26606
                     /gene="livM"
                     /locus_tag="ACHKW0_002092"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HFO7474926.1"
                     /translation="MKPMHIAMALLSAAMFFILAGVFMGVQLELDGTKLVVDTASDVR
                     WQWVFIGTAVVFFFQILRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            26603..27370
                     /gene="livG"
                     /locus_tag="ACHKW0_002093"
     CDS             26603..27370
                     /gene="livG"
                     /locus_tag="ACHKW0_002093"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HFO7474927.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            27372..28085
                     /gene="livF"
                     /locus_tag="ACHKW0_002094"
     CDS             27372..28085
                     /gene="livF"
                     /locus_tag="ACHKW0_002094"
                     /EC_number="7.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HFO7474928.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            28481..29797
                     /gene="ugpB"
                     /locus_tag="ACHKW0_002095"
     CDS             28481..29797
                     /gene="ugpB"
                     /locus_tag="ACHKW0_002095"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HFO7474929.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            29895..30782
                     /gene="ugpA"
                     /locus_tag="ACHKW0_002096"
     CDS             29895..30782
                     /gene="ugpA"
                     /locus_tag="ACHKW0_002096"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HFO7474930.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            30779..31624
                     /gene="ugpE"
                     /locus_tag="ACHKW0_002097"
     CDS             30779..31624
                     /gene="ugpE"
                     /locus_tag="ACHKW0_002097"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015794 - glycerol-3-phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HFO7474931.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            31626..32696
                     /gene="ugpC"
                     /locus_tag="ACHKW0_002098"
     CDS             31626..32696
                     /gene="ugpC"
                     /locus_tag="ACHKW0_002098"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HFO7474932.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKCRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            32693..33436
                     /gene="ugpQ"
                     /locus_tag="ACHKW0_002099"
     CDS             32693..33436
                     /gene="ugpQ"
                     /locus_tag="ACHKW0_002099"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HFO7474933.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(33423..33863)
                     /locus_tag="ACHKW0_002100"
     CDS             complement(33423..33863)
                     /locus_tag="ACHKW0_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HFO7474934.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            33983..35725
                     /gene="ggt"
                     /locus_tag="ACHKW0_002101"
     CDS             33983..35725
                     /gene="ggt"
                     /locus_tag="ACHKW0_002101"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HFO7474935.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAINFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIAEQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(35763..>36024)
                     /locus_tag="ACHKW0_002102"
     CDS             complement(35763..>36024)
                     /locus_tag="ACHKW0_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YrhB family protein"
                     /protein_id="HFO7474936.1"
                     /translation="AKANHYLDDADLPVVITLHGRFSQGWYFCFEAREFLETGDEAAR
                     LAGNAPFIIDKDSGEIHSLGTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         8372 a         9347 c        10004 g         8301 t
ORIGIN      
        1 atatcgttca taataaacct ctttttcagg cgagagcgca cctcgcgtta gcgcctgatg
       61 gagtgggttg aaattaatta ttattaatta atttttaagg atcgaaacaa tttgcgccag
      121 tactttctct tcgccaatac cggtaataat cggcaagccg ttaactaccg ggacgtcgag
      181 ttcataaggc actttagtcg ttgagactac cagtgctacg ccatcagtat ttccaggtaa
      241 agaggcaacg ttggtttggg aataattgac gttaagaccg tgctctttac aatattccat
      301 gactttttct gttaccgccg tggatgtggc aatacccgtg gcgcagacaa ataaaatggt
      361 ttgactcata tttcacctcg ttctttcatt ttgtttaaga ttactgtgtg tatttctgtt
      421 gtttccatcg caagcagggc ttgcataaag ggcgtatcct gaatcatttc cataatccag
      481 cccagggcat ttaattgttt attactttcg cttaaggcga gcaaaaaaat caccctgaca
      541 ggtacggggt caggatcgcc gcccatatcg gcaaactcta ccggttccgg caggatgccg
      601 acggcaattg cattgtgcct gacatgttta tggtccgtgt gcggaatagc gacgcacggt
      661 ggttcggtag gcagccccgt tgggtacgtc gcctcacgtt tcagtacgtg atcggcaaaa
      721 tcagggacga ccagctcggc ggcaaccaac ggttgcgcta actgacggat gatctgttca
      781 acgcttaacc ctgccggaag tcgttgaata taattttcct gaaaatgtat gccttgcatt
      841 ttactcctct taaaaaagtg tacttaactc acttcccgcc gttgcagtac ggtgccaaga
      901 taatatttat ttccttttaa ccagacgttg ccaaactcaa tgggtaatcc tcgggagaaa
      961 aagactaact gctccagatg caaaacgggt gcgtcttcac tgatatctaa aaaatgacca
     1021 cgttcattac caattaatcg cgcggcatag cgactttccg agtaacgaat ttttctttgc
     1081 gacaaacttt ctattgttgg aaataaattg tgttgattaa aatcgatttc cgcaataccg
     1141 gggcatagct caatattgat acggttctct atcagcatcg ctttttcatc accaattgag
     1201 cgtaagcgtt caaggtaaag aatatcctga ccgggcgtta ttcttaattt ctctgccaca
     1261 taacgattag ccgattcaat ccgtgacgta ataacttcag tggtaaaatg tattttctgg
     1321 ctttccagcg attccgcgaa tgacaataat ccttcaccta acggatatgc cacgttctct
     1381 tttttgacaa atgttccctt accctggacc tgtattaaca cgccttcttt taccagttgg
     1441 gcgaccgctt ttttaacggt tccccgactc acttccagca tatccataat ctggtattcc
     1501 gaaggaatgc gatcgtcttc caccagatct ccggtataaa tactttctct tatccactcc
     1561 accacttgtt gatagagcgg tatttgtgac gacttactta atgatttcat ggcgcgcacc
     1621 tgaacattta cagttgtcta tggacaatca tggacaactt caagcgtata gtaggtgaac
     1681 gggagaaaaa gaggatcgaa gcgtctcaca ttttgaggga ccaaatcgtg atcaaaaaaa
     1741 tgtgacgtga gtcgatattt gtcagaagtt tactgcgccg taaggaaata acggcgcgtt
     1801 ggtcggtgtt aatcttcctt cggcaagcac tgcaaatgcc cgtgccgcac gccgcgctgg
     1861 gcgataacgt catcggcaaa atgctgcacg tcacccatgt cacctttcaa cacggcgatt
     1921 tccagacagt cgtcgtggtt gatgtgcaca tgcagcgtgg cgacggagag gtcgtggtga
     1981 tgatgctggg tggaaacaat acggctggct aagtcgcgtt tttcgtgttc atacacatac
     2041 gacagcaccg cgaaaccttg tgtgccgtgc tgctgggtag cttcttgcgc cagggcgcta
     2101 cgcagaatat cgcggatagc ttcggaacgg ttgttataac cacgacgctg gctcaggctg
     2161 tccagcgtct ccagtaaatc gtcatcaagc gtgatggtga ctcgttgcat ttgcgttaaa
     2221 ccttttctgt ggtgcgacgg cgcacgggga atgcgggtaa taccgcgttt tgtagcacac
     2281 gtccggcgtc agaggaaaag gttaatttat ctcccaccac ctgggtttcg acgatttgtc
     2341 cgttgtccat caccatcacc cgctgacaga agcgttccac caggcgtaag tcgtgggtga
     2401 tgaacaggca ggcggtgcca aactgttgtt gtagcttttt cagcaggcga atgacgcccg
     2461 cctgtaacac gagatcaagg ttagaaacgg cttcatccag aatcagtagt ttcggttcga
     2521 ccgccagcgc gcgagccagg cagacgcgct ggagctggcc gccgcttaac tgcggtgggc
     2581 gtttgtcgag aacgctgtca tcgagatcga ccgccttcag catttcgctg gcgcgcgcca
     2641 gttgttcgga ttttttcagt gatagcaggt ggcgcatcgg ttcacgcagg atctcgcgca
     2701 cggttttgcg cggattcacg gcgctgatgg agtcctgaaa taccatctga atatcgcggc
     2761 ggaacgcttt acgctgggcg cgattgagtt tcgccagcgg ttcgccgcgc cagctaatat
     2821 tcccttgtga gggagactct aagcctacca gcagccgcgc gagggtgctt tttccgcagc
     2881 cgctgcgtcc cagcagggcg acggtttcgc cgcttttcag ggtcagggaa acgttattca
     2941 gcaccgcctg atgttgatgt tttccgctaa atccaccgtg ggcatagtga tgggaaaggc
     3001 cgcagacgtt aagtaaggtc atgatgccag ctccataccg tagagggcga gatgagcgga
     3061 aaccaggctg cgcgtcaccg tatgtttggg ggcgttaaac agcgtttcta catcgccctg
     3121 ttcgacaatt ttaccttgtg acatcaccgc cacgtcatcc gccagacgcg ccacaacgcc
     3181 catatcatgg gtgaccagca gcattcccgg cgcttgtttt tgcataatgc tttccagcag
     3241 atcgaggatg cgcgcctgtg ctaccacgtc gaggtcggtg gtcggttcat cggcgatgat
     3301 aaacggtgat tcacacagca ccgccatcgc aatcatcatg cgctgcaaca tgccgccgct
     3361 catctcgaac gggtacagct tcagcacgcg cgcggcgttt tccagcccca ccgcttctat
     3421 ggcagcggta agcgtagcgt catcggcggg tttccctaac gccaggcagg tttcccgcgc
     3481 gtgggtgtgc atggtgtgca gtggattaaa ggcgctgcgc gggttctgca tgatggtggc
     3541 aattttgatg ccgcgcaggg cgcaaggcga aaccggtttg ccatcggcta aaatttcccc
     3601 cgccgtctgg cgaacgccag cgggcaaaat gcccagcgtc gcggcgcagg ttaatgattt
     3661 cccgctgccg ctaccgccga ctaacgccag cacgcgcccg cgttgcaggg ttaacgatac
     3721 accgtgtacc agcggctgcg cggcctgtag cgcgatatta cgtagttcaa tctgttgcgg
     3781 cattagtgtg cgtgctccgt caccagatga gggtccagat gatcgcgcag tgcgtcaccc
     3841 accaggttaa aggccatcac gctgataaac agcgccagcc ccggccagaa catttgcagc
     3901 ggctgggtcc agatatactg gcgcgcgtcg ttaatcatca cgccccattc ggcggtcggc
     3961 gcggtcacac cgaggccaag gaaagacatc cccgcgacgt gcagcatcat atggccgata
     4021 tccagcgttg ccagcaccag cagcgaaggg atcaccgcgc ctgccagatg atcgacaaac
     4081 acccgcacat ggcccgcgcc agaaagccgt gacgccagca caaactcgcg ttggcgtagt
     4141 gaaatcacca ggctgcgcac catgcgtgca taccacgccc agtgcgacag ggcgatggcg
     4201 ataattacgt tggtcagccc ggtgccgagc acgccaacca taaagaacga cagaatcgag
     4261 gtcgggaagg tcataaacat atcggcgacg cgcatggtgg cctgatcaac gcgcccgcca
     4321 atcaacccgg cgctgccgcc aataaccagc cctaatgtca gcaccagcag caggcaggcc
     4381 attaccgaac cgagcgacac gcgggtcgct gccatcagcc gcgagaaaat atcgcgacct
     4441 aagtgatcgg tgcccagcca gtgctgcgca tccggcgaaa gcaggcgcga cggcaaatca
     4501 atcgcctggg ggtcatacgg cagccaccac tggctggtga gcgcaatcag cgccagcagg
     4561 gcgataatga tcagcgccag gcgtaccgac cagcgggaag agaggaaaaa gttcacgcgt
     4621 gcgctccttc atgacgacga atgcgcgggt ccagcgcggc gttgagcaaa tcgacaatca
     4681 aattacagac cacaaaaacc accaccatca tcagcgtaaa gcactggatc accggatagt
     4741 cacggttaaa aatcgccgac accgcatagc gcccgacgcc cggccaggca aagatgtttt
     4801 cgataatcat cgtcccgcca atcagttcgc cgatgtgcat ccccacggcg gtgatcatcg
     4861 gcagcgaggc attgcgcagg atgtggcgac gttcggtctg tttgtcgttc aggccgcgca
     4921 gacgcgccca ggtgacgtga cgctgaccgg cgacgtccag catactggcg cgcagtaaac
     4981 gcgcgttaat cgccagcgac ataaaggcaa tggaaaccgc aggcaaaatg atgtgctgcc
     5041 agccgccgta acccatcgcg ggtagccatt gcagatacac cgaaaacgcc atcaccagta
     5101 aaaacgccag ccagaagtta ggcatcgaca cgccaagaaa cgcgatgaaa cgtacggcga
     5161 aatccggcag acggtcgcga tggcgcgccg cccagatacc gagcggtacg gaagtgagca
     5221 gaattaatac cagcgccgca cctgcaagtt ccagcgtggc gggcaggaag ttcagcatat
     5281 cgtccagcac cgggcgttgg ctggcgaatg agataccaaa gtcaagatgc agcgccttcc
     5341 acaaccaggt gccgtactgg acgtacagcg gctgatccag cccgagcatg gtgcgggtag
     5401 aggccagcat ctccggcgtc ggcggcaggt tagataaacg cagataatcg agcgccgggt
     5461 cgccggtacc gaggcgcagc atcagaaaaa tgatcaccga ggcggcgagc accatcggga
     5521 tcagcagcag aaagcggcgt aatacgtaac gcaacattaa ggtttcaccg gtttaatctg
     5581 ttcgaacgga atttcagtgg cgatcggcgc gtaggggatg ttacccagct ccggttttga
     5641 taccaccatc attgagatgt aactgatagg cagataaacc gcctcgtcat gcagacgggt
     5701 cagaatgtcg cgatacagcg cctgacgttg cgtttcgtca tgggtcgcca gcacttcgcc
     5761 gatctcttta tcaatcagcg gtttgtcggc taatccttgc tgtgcctgga agtcagcgtg
     5821 tgacggtacg cgcattgaac tgaggaaggc gtgtggatcg tatggcgcgc cccaggtgcg
     5881 gtggaaaatc atgccaaaac gaccgtcgcg ctgacgagca tagatactgc tctcttcttc
     5941 gccaatcagc gagacatctg cgccaatctg gcgcatatca gcctgaatga tttccgccat
     6001 cgatttgctt aacgcatcgg tgccgatgaa cgacagttca atgcgcagcg gctgaccatt
     6061 tttctcgcgg atgtctttgc ccgcaggcag cgtccaaccg gctttttcca gcaacgcttt
     6121 cgctttctgc ggatcgtact ggcgcggttt caggccgagg ttggcgtagg gcacagaagg
     6181 ggcaaacagg gtgtcggcga cctgctgggt gccatacaac gcgttatcaa tcagcgattt
     6241 tttgtttacc gcgtaattaa gagcttcacg tactgccagc tcattggtgg gggctttggc
     6301 ggtattgagc gccagcatca cggtttcgat cggctgtgac agttgggtgt ggtaagccgg
     6361 attctggcta aagcgggcga aggtatcgag cggtaataac ccttcgtttc cgtacagcag
     6421 gtcgatatcg ccagtttcaa acgccaccgc gcgggtagtc gggtccggga tgacgttaaa
     6481 ggtgatcttt ttaatcgctg gcttttcgcc ccagtagttt tcgttacgga cgaagacatc
     6541 gtactgattc agtttcgatt cctgcaaaat ccatggtccg gtgccaatcg gcgctttaat
     6601 tccgttcatg gtttcatggt ttttaaactg cgagggagcg ataaaacgga aaggacgggg
     6661 cagggccagt tcttgcagga aaggatagta ggcgcttttc agggtaattt gcagctctgt
     6721 tttactgagt gctttaacat caacaatctg gtttgccagc tccagccagg cgtgacgttg
     6781 acggttatcg agcactgcgc ggaagttttc tgccgccgcc tcggcatcga acggttcacc
     6841 gttggagaat ttcacgtcat cacgcagggt gaaggtccag gttttaccat cttctgaatg
     6901 agtccagctt tttgccagcc acgggatcac cgaaccgtct gcctgatatt tcaccaatgg
     6961 ttcataaacc atgctctggg cgaacatctg gttaggcgtg taaaggtgcg ggtttagtgg
     7021 cccgacattc accggccagg cggtagtgat ttcatctggt gcagcggcat ggacgataaa
     7081 agacgcacaa gccagcagcg caaatagagt gcggcggagt gtggagagca tggtaacccc
     7141 aatggattaa aatagatggc ggaaataagt atgacgattt taagtattcg tcatactgat
     7201 tacctgttct ggatcaatag atgggcagtc gggtgggcgg atcagttaac tgaatcgatc
     7261 cattgcacac tgtcggcggt gagcgtaaag ggggtggggg tgcagatcgc caggcttaaa
     7321 ttttcaagct gacaatggct gactgacagc gaggagtgat aggtgctgtc tacgctgacg
     7381 atttgccagg cgctgccgcc acgctgttta acgatggctt ctttgcgcgt ccagatgcgc
     7441 cagaacattt ccagttgctg atcaggatgc acggcgtcca tctcagcgtg ttccccgagg
     7501 ctgaatacgg cgttcgccag ccagcgccag ttggcgcgcg ggcgaatcac ttcgatatcg
     7561 cagccgactt cgccttcatc actcaacagc agagcgatat cgtcaccgct atggcttaag
     7621 ttgaaccata gcggcgtttc cggcgcgaat gcaggtttgc cttgttcgcc atagatgatc
     7681 tccggtagcg gggaaagcgt gtgcgaaagc aatgcacgcc ccgccagcca gcgttcgcgt
     7741 cgtggacctt gcggtgcttg ctcgcgtaaa cccggtggca gtggagctgc gcttaaggtc
     7801 gaaactttcc ccagaactat ccgatacata tcagggccaa cgtttaatgg aaaatgaaag
     7861 tgcgtatcgt atcacttgtc gcctcatccc ggtaaccgac ttttcggtct gcccggcccc
     7921 agtaaaatcg ccagtttgct gccacctttc gtcgtttcca tccagatttt acacacgctg
     7981 gttaatggca ccgaaagtag catccctacc gggccgagca gccatcccca gattaacaat
     8041 gaaagaaata ccaccatggt ggacatcccc aggcgatggc ccatcatccg tggctctaaa
     8101 atattgccga tgaccatatg gacaactaaa aacaatgcgc cgaccagaat acattcgtaa
     8161 ataccattaa acagcagcac ctgaatcatt ggcggtacgg cggaaattac cgcgccgata
     8221 ttgggcacgt agttaagcaa aaacgccagt actgcccaca tcagcgcaaa ctgtaccccc
     8281 atcagctcca gccccagcca gacgatgaca cctgtccata aactgagtag cgtcttcaat
     8341 gcaagatagt gcgaaacgcc tttaagtgcg cggtgtaatc ccgcgatgtg aatctgtggg
     8401 ttattcagcg caaaacgcat tttgtaaggg acgtggcgca cttcaaacag cataaaaact
     8461 acggtcatca ccagcaaaag cacgctcgcc attgccccgg aaagcccggt cattagcgct
     8521 gtggtgaagg taaccacttt ttccgagtcc atccgctgca gcattcgctc cggcgacata
     8581 tgcaaattaa gaaaaggcaa catctcctgc aatttaaaaa gtttgcgcgt caactcctta
     8641 ttaaacttcg gcagcataga gataaattcg ttaaacgatg ccgccagtac gccgaccagc
     8701 gcagttagtg cgatcagcat caccaccacc acaatcgtaa tggcaatggg gcgttgtact
     8761 ccccgacgaa taaaccaggt gacgagcggg ttgaggacga tggcaaaaaa cagcgccagt
     8821 aacagctgca caatgatatc tgccgctgcg tgaatgcccg cgaggatcac taccagcgag
     8881 gccagcttga gcagaatgtg catgcccgtt ttatcgggtt gaggggtttc cattggggct
     8941 tccttgtgac tttttgtatt aagtgtagcg ggagtcacgc cagcaatatt ctgattctta
     9001 ctgctgattt tcacgtcagc acgtggtaaa agtgaaacac tgttgtaaaa atgtggtgat
     9061 cctcatgccc gaacccgtag ccgaacccgc gctaaacgga ttgcgcctga atttgcgcat
     9121 tgtctctata gtcatgttta acttcgccag ctacctcacc atcgggttgc cgctcgctgt
     9181 attaccgggc tatgtccatg atgtgatggg ctttagcgct ttctgggcag gattggttat
     9241 cagcctgcaa tatttcgcca ccttgctgag ccgtcctcat gccggacgtt acgccgattt
     9301 gctgggcccc aaaaagattg tcgtcttcgg tttatgcggc tgctttttga gcggtctggg
     9361 atatctgacg gcaggattaa ccgccagtct gcccgtcatc agcctgttat tactttgcct
     9421 gggacgcgtg atccttggga ttgggcaaag ttttgccgga acgggatcga ccctgtgggg
     9481 cgttggcgtg gttggctcgc tgcatatcgg gcgggtgatt tcgtggaacg gcattgtcac
     9541 ttacggggcg atggcgatgg gtgcgccgtt aggcgtcgtg ttttatcact ggggcggctt
     9601 gcaggcgtta gcgttaatca ttatgggcgt ggcgctggtg gccattttgt tggcgatccc
     9661 gcgtccgacg gtaaaagcca gtaaaggcaa accgctgccg tttcgcgcgg tgcttgggag
     9721 cgtctggctg tacggtatgg cgctggcact ggcttccgcc ggatttggcg tcatcgccac
     9781 ctttatcacg ctgttttatg acgctaaagg ttgggacggt gcggctttcg cgctgacgct
     9841 gtttagctgt gcgtttgtcg gtacgcgttt gttattccct aacggcatta accgtatcgg
     9901 tggcttaaac gtagcgatga tttgctttag cgttgagata atcggcctgc tactggttgg
     9961 cgtggcgact atgccgtgga tggcgaaaat cggcgtctta ctggcggggg ccgggttttc
    10021 gctggtgttc ccggcattgg gtgtagtggc ggtaaaagcg gttccgcagc aaaatcaggg
    10081 ggcggcgctg gcaacttaca ccgtatttat ggatttatcg cttggcgtga ctggaccact
    10141 ggctgggctg gtgatgagct gggcgggcgt accggtgatt tatctggcgg cggcgggact
    10201 ggtcgcaatc gcgttattac tgacgtggcg attaaaaaaa cggcctccgg aacacgtccc
    10261 tgaggccgcc tcatcatctt aaaacttact gaataaccag cgtattaatg atgttttctg
    10321 cggtggtctg cgctttttgc tgatcgtcag cgggcagcgt aatttgcatg gtcagcagtt
    10381 gattacccac gttacccaga ataacggaag agtacgccgt ctggcctttc gcggagataa
    10441 tactgtctaa ctgctgcatt ttgtgacctt tcagctcaat ggctttattg gttaccactt
    10501 gcagctgcgg atcgcggcta cgttgctgat cttccagacg cttcgccagc accgccagat
    10561 cttctttcgg atcatcgccc atgatgacga tgactgcttt ctgcccggtg gcgtcggacc
    10621 agacatgcat gttattggcc tgcgttccca gcttaccgct ctggtcggtc atatccgctg
    10681 gcagcgagaa acttaacttg ccatcaagca ggttgacggg attcccggta gcgttacttt
    10741 ccgcgaccga accctgcgcc gtagcgttag tgtctttatc atcacaggcc gcaagcccca
    10801 taaccagcag gccaattccg acatatttaa ccagattgcg cattgacttc ttcctttcga
    10861 taaacggcca taacggctca ttcatccatc ttatcacaac tctgataacg aacctttaac
    10921 tcgcctgcaa agcgttgatt tcggatttat ctgccagtct tttcaacagc atattgagta
    10981 atacgccata cattggcagg aagaaaacga tactgattaa cactttgaaa cagtaatcga
    11041 ccagcgcgat ttccatccag tgttcagcca taaaggcatc cgggctacgc cagaaggcaa
    11101 tgaagaaaaa ggccagcgtg tcactgacgt taccgaacag tgtggacgct gtcggtgcca
    11161 gccaccagcg gcgactttga cgcaggcggt taaaaacgtg cacgtcgagg atttgcccca
    11221 gcgcgtaggc catgaaactg gcggtggcga tacgggcgac aaacaggttg aagtgggcga
    11281 gtgcgccgaa tccctgccat gaacccatat agaacagcga cgagatgaca taggagatta
    11341 ataacgcagg gatcattacc gcgaagataa tgcgtcgggc cagcggtgcg ccaaaaatac
    11401 gcacggtcag gtcggtcgcg agaaagataa acggaaaact aaacgcgccc caggtggtat
    11461 gaaaaccaaa aatggagacc ggaagctgga ccagatagtt actggaggtg atcaccagca
    11521 gatgaaataa cgataaccag aacaacgcct tatagcgttg agtttgcgag aaaacgttca
    11581 tattgtacct ttttgattaa ccattggggt gagggaaccc aatacgtacg acacgtctgt
    11641 tatcttaaca taataacgtg cttacccttt tttcgagccg ccgcatgata ctgctttacg
    11701 ttgacaatgc aatggttaat tttcacgcaa tcgttaacct ggtttgctta cggactcaca
    11761 gggcgtaaac tagcgccgtt tttttaagtg atgagaagaa aatgaccgat ctcttttcca
    11821 gccctgacca cacactcgac gcgcttggcc tgcgctgccc ggaaccggtg atgatggtgc
    11881 gcaaaaccgt gcgcaatatg cagcctggcg aaactttact gattatcgcc gacgatccgg
    11941 cgacgacccg cgatattcct gggttttgta cctttatgga acacgaactg gttgcgaaag
    12001 agacggatgg actgccttat cgttatttga ttcgtaaagg cggttgatgg ggaaagattg
    12061 gcttcgatgc cgccttttcc cctcacccta accctctccc cagaggagcg aggggaccga
    12121 tcgcgctcaa tgttgcgatc ggtttgcctt atctcctgcg caacaatctt aacgcattcg
    12181 ctgttaccag cacggtcgca ccggtatctg ccagcaccgc cagccatagt ccggtcatcc
    12241 ctagcagcgt ggtgacgagg aagatccctt tcagccccag cgcaatagtg atgttctggc
    12301 ggatattggc gtgagtggcg cgtgccagtt caatcatttg caccaggccg cgcaggtggt
    12361 tatgggttaa tgctgcgtcg gcggtttcca gcgccacgtc tgtgccactg cccattgcaa
    12421 tcccaatggc ggcagctttc atcgctggcg cgtcgttaat accatcaccg accatcgcca
    12481 ggggcgcatg ttgattcagc ttggtcaccg ctttgacttt atcttccggc aataggcccg
    12541 ctttaaactc cagccccagc tccccggcaa ttgccgccgc tgcgcgtgga ttatcgccgg
    12601 tgaggatcac tcctttaacc cccagcgcgt tcagttcact gatggcagtt gctgcgtcgg
    12661 cgcgcagggt atcctgcaat gcaatgatac ccagcacgtc atcattacgt actaccagca
    12721 caaccgtttg cccggcgctt tccagttcat taatcaaacc agcaaatgca tcagcgggat
    12781 gtttcccggc agcgcaaatc aatacgcgct caccgttaac ctgcgcttca atgccagacc
    12841 cgaccagcgc ccgctgtgat tcggcggtgg gaatggcgag ttcagcaacc tgtgcttcgc
    12901 gtacgatggc ctgcgccagt ggatgcgtcg cgccttgctc gaccgccgcc gccagtgtca
    12961 gcagttcaga ttcactaata cccgttgccg gatgaatcgc ggtaacgcgc ggtttaccga
    13021 cggtcagcgt accggtttta tcaaacgcca cctgagtaac acgacccagc tgttccagcg
    13081 ccgctccgcc tttaatcaac gccccacgac gcgctgccgc cgccagcccg gaggtaatcg
    13141 ccgcaggcgt ggagataact aacgcacacg ggcagccaat cagcagcagc gtcagccctt
    13201 tataaatcca ctcctgccag ctggcggcaa acagcagcgg tggcaccagc gtcaccagca
    13261 gggcgacggc cataatcgct ggcgtataaa tacggctgaa acggtcgata aaccgctcaa
    13321 tgggggcgcg gcgctcttcg gcttcttcga tcagtttcag aatccggtca atggcgctgg
    13381 cacccggttc tgacagcact tccagcgtta ccagacggtc tacgctggtg gctcctgcag
    13441 gaactttatc gcccgtcgcg cgctccaccg gaatggattc tccggtcagg gcgctttcat
    13501 caaaactggc aaacggtgag agcagtttac cgtcggcagg caaacgccca cctgcggcga
    13561 cttcaatcac atcgccaggc cgcaggctgt taatcgccac ctcttcccgc tcaccgttac
    13621 gcaggcgcgt ggcggtttct ggtttcagcg ccattaacgc gctaacgccc tgacgcgcgc
    13681 ggctggcggc ccagccttcc agtcgttcac caatcaaaaa cagcagcaac accatcgcag
    13741 cttcagccgt tgcgccaata aacagtgcac caatagcggc tacgctcatt aaggtttcaa
    13801 tggcgaagta gctgccggat ttgatcagcc gtaatgcctg acgagcaatc gggtacagcc
    13861 caaccagcgt ggtcgcgata aacgccagtt gcccaaacgg atgattaaat tgctccagac
    13921 cccagctgat tgccatcatc acgattagcg taatcagcgg cagattctct ttcaggcgcg
    13981 atgcttgtgg ctcgtcggcg gcctgctcat cgcgcaggga atagcccgct ttttgcaccg
    14041 cagattcaac ctgggcacgg atgtcattgt cggcatcgac caccagtttt tcggtggcga
    14101 acagtacctg cacctgattc acacctgcaa gctggcgcac ggcattttct accttgcgcg
    14161 cacaggcggc gcagtccatg ccgctgactt tccagctata gcgggtgccg gagacgtttt
    14221 cagagagcgt tggcgagctg gaacatgcgc cgtcgcagca acagtcgttg gcgttctgta
    14281 ccgtggttag cggtttgaac gcagcaaatt gaggggcttt cttgccgtga ttgtcaggag
    14341 tcgacatggc atcctccggt taagtttttt ctcattaacc gaaggataca ctctggagtc
    14401 gactccagag tcaagtttta tcagagatac agcgagcgga cgatgaggaa atgaccggca
    14461 aagtagcagg ccgcagcaat cgcgttatct gcccggaagc ggcggcgata gtggctaccc
    14521 agccagacaa agttactgat aaacagcaat gaagcgccaa caaacgcaga gagtgccgga
    14581 gcggtcggac ggaagaacca cagttcacct gccagccaca ccatcaccag cgtcatgccg
    14641 ataaacgtgc agataggcca acgatactct tccagtcgcg tccagataat cgccagtaac
    14701 agcgcgccca gaaccagcaa caccagtggc agaggccaga agaaagagag cgtcatctga
    14761 ctggcgaaat agatggtgta cagcaggtgc gagaggaaaa acgcgccgat ggcgtacatc
    14821 agacgttgac gtggcaacag ggttagcgca tcgcccagca gtgaggcgca cagccctgcc
    14881 agcaccagat agctaatggc gtcgaacatc ggcgcttgcc aggccagtaa cagcaggaga
    14941 agaagggtta acggtttaaa cacccagcgt tgccaggttg gcccacgata cgatgcatcc
    15001 acagatagcc atgcggaaag acagacagcg ataaacgacc aaagcatctt agttccttga
    15061 attcgttatt ttttcgtaag cggaatgcct acggtcttgc ttccagttta gtggcgaggg
    15121 tggacggatg acaacaccat aactggcagg gtatgcttat ttccgcattt tccgatgagg
    15181 gataaagatg agcaaaccac ctcttttctt tattgttatc attggcttaa ttgtcgtcgc
    15241 cgcatcgttt cgttttatgc aacagcgacg ggaaaaagct gataatgata tggctccgct
    15301 ccagcaaaag ctggtggtgg tgagcaacaa gcgggaaaaa ccgattaacg atcgccgttc
    15361 gcgccagcag gaagtgactc cggcaggcac cagtatgcgc tatgaggcaa gcttcaaacc
    15421 gcaaagcgga ggaatggagc agacgtttcg cctcgacgcc cagcagtacc acgccctgac
    15481 agtgggcgat aaaggtacgc tgagctataa aggaacgcgc tttgtcagct ttgtaggcga
    15541 acaataaggt tattttttaa ctttaaattt cttctgccag accagcagtt caaacacgcc
    15601 gaaaaggaaa atccgcacct tttcggcggt ggtcatctgc gggccatctt tcggtaaagt
    15661 ggatttcaat agcgccagtt gcataccatg catcagcacg gtaaaaatca gcgccacgtt
    15721 aacgaaaata ttcagcgggc gcgggaaggg atgcaccaga ttgaggatta aaaatcccca
    15781 aacgcagagc attaacaaac gaccaatatt aatcagcatc actttctcct tgtgcttcgc
    15841 gttgatacag ccgataagcc acctgacccg ccactttttc ccgatgtaat gaccagtttg
    15901 ccggaacagt gggcagaccg ttttcgactt cgctttcgac ataaatcagg gcttcgtcag
    15961 ccagccagcc gttatcttcc agtaaattta tcgtctcttc taacaagcca cggcggaacg
    16021 gtggatcgac aaacacgata ttatgcggtg tgcctttttg cgccaggaat gacattgcgt
    16081 tgctgttcac cacgcgtgca ttgcctgctt ttagtgtcgc cagattctta attaactgct
    16141 gagaaactgc gcgatccatc tcaatcaacg tggcccccgc agcgtagcgc gataacgctt
    16201 ccagccccag cgcgccgctc ccggcgaagc aatccagaca ttgggcgtca acaatgaccg
    16261 gagccagcca gttaaacaac gtttcgcgta cgcggtcggt ggtggggcgc agacctgggc
    16321 tatcaggaac cgggagttta cggcctcgcc actgcccgcc aataatgcgg atttggccgc
    16381 tgccggaatg attcggtttt ttcatgataa attgctcaat ccgccagata acacatactt
    16441 gcaggcggtg atgtgaatta agttaagtga tagactattt catcattttt ttagctgcta
    16501 tgtacatagc gttaacgctg tgccatgaag caacagcgag gagtgtagtc gcaaatggcg
    16561 aaagaaaaaa aacgtggctt tttttcctgg ctgggctttg gtcaaaaaga gcagaccccg
    16621 gaaaaagaga cagaagttca gaatgaacaa ccggttgtag aagaaatcgt tcaggcgcaa
    16681 gagcctgtga aggcttctga acaagccgtc gaagagcagc cgcaggcgca tactgaagcc
    16741 gaggcggaaa cttttgctgc cgacgttgtg gaagtcactg aacaggttgc tgaaagtgaa
    16801 aaagcgcagc ctgaagcgga agtcgttgca cagccggaac cggtcgtaga agaaacgccg
    16861 gagccagtgg ctatcgagcg tgaagagctg ccgttgccgg aagacgtcaa cgccgaagag
    16921 gtttcgccag aagagtggca ggctgaagcg gaaaccgtag agattgtcga agcggcggaa
    16981 gaagaagcgg ctaaagaaga aatcaccgac gaagagctgg aagcacaggc gctggctgcc
    17041 gaagcggcag aagaggcggt gatggtggtt cctccggtag aagagcagcc ggtggaagaa
    17101 atcgctcagg agcaggaaaa accgaccaaa gaaggtttct tcgcgcgcct gaaacgcagc
    17161 ctgttaaaaa ccaaagaaaa tctcggttcc ggatttatca gcctgttccg cggtaaaaaa
    17221 atcgacgatg atctgtttga ggagctggaa gaacagctgt tgatcgccga tgtgggcgtg
    17281 gaaaccacac gtaaaattat caccaatctg acggaaggcg catcccgcaa gcagcttcgt
    17341 gatgccgagg cgctctatgg cctgctgaaa gaagagatgg gcgagattct ggcgaaagtc
    17401 gatgagccgc tgaatgttga aggtaaaacg ccgttcgtga tcctgatggt gggcgtcaac
    17461 ggtgtgggta aaaccacgac gattggtaag ctggcgcgtc agtttgagca gcagggtaaa
    17521 tcggtgatgc tggcggcggg cgatactttc cgtgcagcag cggttgaaca gcttcaggtc
    17581 tggggtcagc gcaacaatat tccggtgatt gcccagcata ctggtgcgga ttccgcctct
    17641 gttatcttcg acgccattca ggcagctaaa gcgcgtaata tcgacgtcct gattgccgat
    17701 accgccggac gtctgcagaa caaatcgcac ctgatggaag agttgaagaa aatcgtccgc
    17761 gtgatgaaga aactcgacgt tgaagcgccg catgaagtta tgctgactat cgatgccagc
    17821 accgggcaga acgcggtaag ccaggccaaa ctgttccatg aagctgttgg cttaaccggc
    17881 atcacgctaa cgaaactgga cggcacggcg aaaggcgggg taattttctc ggtggctgac
    17941 cagtttggta tccctatccg ctacattggt gtcggcgaac gtattgagga tttgcgtccg
    18001 tttaaggcgg acgactttat agaggcactt tttgcccgag aggattaaca atgattcgct
    18061 ttgaacatgt cagcaaggct tatctcggtg ggagacaggc gctgcagggc gttacgttcc
    18121 atatgcagcc gggtgagatg gcgtttctga ccggtcattc cggcgcaggg aaaagtaccc
    18181 tcctgaagct gatctgtggg attgagcggc ccagcgccgg gaaaatctgg tttagcggcc
    18241 atgacatcac gcgtctgaaa aaccgtgaag ttccgtttct gcgccgccag attggcatga
    18301 ttttccagga tcaccatcta ctgatggacc gtactgtcta cgataacgtg gcaatcccgc
    18361 tgattatcgc cggtgccagc ggtgacgata ttcgtcgccg ggtgtcggcg gcactggata
    18421 aagtcgggct actggacaaa gcgaagaact tccctattca gctttctggc ggtgaacaac
    18481 agcgtgttgg cattgcccgc gcggtggtga acaagcccgc ggtactgctg gcggacgaac
    18541 cgactggtaa cctggacgac gcgttgtcgg aagggattct acgtctgttt gaagagttta
    18601 accgcgttgg ggtaaccgta ttgatggcaa cgcacgacat caacctgatc tcgcggcgtt
    18661 cctatcgcat gctcaccctg agcgatggtc acttgcatgg aggcgtgggc catgaataag
    18721 cgcgatgcaa tcaatcatat tcggcagttt ggcgggcgtc tggatcgctt ccgtaaatcg
    18781 gtcggcggct ctggcgacgg cggtcgtaac gcgccaaaac gcgcgaaatc ctcgccaaaa
    18841 ccggtaaatc gcaaaaccaa cgttttcaac gaacaggtgc gctatgcctt ccacggcgca
    18901 ttgcaggatc tgaaaagcaa accgttcgcc acgtttttaa cggtgatggt tatcgccatc
    18961 tccctgacgc tgcccagcgt ctgttatatg gtgtacaaaa acgttaacca ggcggcgacg
    19021 caatattatc cgtcaccgca aatcactgtt tacttgcaaa aaacgctgga cgatgacgcc
    19081 gcagcaggcg tggtagcaca gctacaggcc gagcagggcg tggagaaagt gaactatctt
    19141 tctcgtgaag acgcactggg cgagttccgt aactggtctg gttttggtgg tgcgctggat
    19201 atgctggaag aaaacccgct tccggcagtg gcggtggtga tcccgaaact cgattttcag
    19261 gggacggaat cactgaatac gctgcgtgat cgtatcaccc agattaacgg cattgacgaa
    19321 gtgcggatgg atgacagctg gtttgcccgt ctggcggcgt tgaccgggct ggtcgggcgc
    19381 gtttccgcga tgatcggcgt gttgatggtg gcagcagtgt tcctcgtcat cggcaacagt
    19441 gtgcgtctga gcatctttgc tcgccgtgac tccattaacg tgcagaaact gattggtgcg
    19501 acggatggtt tcatcctgcg cccgttcctg tacggaggcg cattgctggg attttctggc
    19561 gcattgctgt cattaatttt gtcagaaatt ctggtactgc gtttgtcatc ggcggttgca
    19621 gaagtggcac aggttttcgg aacgaagttt gatatcaatg gtttatcgtt cgatgaatgc
    19681 ctgctattgc tgctggtatg ctcgatgatt ggctgggtgg cagcgtggct tgccacggta
    19741 caacatttac gccactttac gcctgaataa taaaagcgtg ttatactctt tccctgcaat
    19801 gggttccgta gcagggaaag agatcccgtt gtctcttccc ggtatttcat ctctatgtca
    19861 cattttgtgc gtaatttatt cacaagcttg cattgaactt gtggataaaa tcacggtctg
    19921 ataaaacagt gaatgataac ctcgttgctc ttaagctctg gcacagttgt tgctaccact
    19981 gaagcgccag aagatatcga ttgagaggat ttgaatgact gacaaaatgc aaagtttagc
    20041 tttagcccca gttggcaacc tggattccta catccgggca gctaacgcgt ggccgatgtt
    20101 gtcggctgac gaggagcggg cgctggctga aaagctgcat taccatggcg atctggaagc
    20161 agctaaaacg ctgatcctgt ctcacctgcg gtttgttgtt catattgctc gtaattatgc
    20221 gggctatggc ctgccacagg cggatttgat tcaggaaggt aacatcggcc tgatgaaagc
    20281 agtgcgccgt ttcaacccgg aagtgggtgt gcgcctggtc tccttcgccg ttcactggat
    20341 caaagcagag atccacgaat acgttctgcg taactggcgt atcgtcaaag ttgcgaccac
    20401 caaagcgcag cgcaaactgt tcttcaacct gcgtaaaact aagcagcgtc tgggctggtt
    20461 taaccaggat gaagtcgaaa tggtggcccg tgaactgggc gtaaccagca aagacgttcg
    20521 tgagatggaa tcacgtatgg cggcacagga catgaccttt gacctgtctt ccgacgacga
    20581 ttccgacagc cagccgatgg caccggtgct ctatctgcag gataaatcat ctaactttgc
    20641 cgacggcatc gaagatgata actgggaaga gcaggcggca aaccgtctga ccgacgcgat
    20701 gcaaggtctg gacgaacgca gccaggacat catccgcgcg cgctggctgg acgaagacaa
    20761 caagtccacg ttgcaggaac tggctgaccg ttacggtgtt tccgctgaac gtgtgcgcca
    20821 actggaaaag aacgcgatga aaaaacttcg cgccgctatt gaagcgtaat ttccgctatt
    20881 aagcagagaa ccctggatga gagtccgggg tttttgtttt ttgggcctct acaataatca
    20941 attccccctc cggcaaaacg tcaatcccca cgcagattgt taataaactg tcaaaatagc
    21001 tattccaata tcataaaaat ggggtatgtt ttagcagagt attctgctaa agcacgggta
    21061 gtcatgcata aaacgaaata aagtgctgaa tcacaacacc acaacacacg taataaccag
    21121 aagaatgggg attctcagga tgaacataaa gggtaaagcg ttactggcag gatgtatcgc
    21181 gctggcattc agcaatatgg ctctggcaga agatattaaa gtcgcggtcg tgggcgcaat
    21241 gtccggtccg gttgcgcagt acggtgacca ggagtttacc ggcgcagagc aggcggttgc
    21301 ggatatcaac gctaaaggcg gcattaaagg caacaaactg caaatcgtaa aatatgacga
    21361 tgcctgtgac ccgaaacagg cggttgcggt ggcgaacaaa gtcgttaacg acggcattaa
    21421 atatgtgatt ggtcacctct gttcttcatc aacgcagcct gcgtctgaca tctacgaaga
    21481 cgaaggcatt ttaatgatca ccccagcggc aaccgcgccg gagctgaccg cccgtggcta
    21541 tcagctgatc ctgcgcacca ccggcctgga ctccgaccag gggccgacgg cggcgaaata
    21601 tattcttgag aaagtgaaac cgcagcgtat tgctatcgtt cacgacaaac agcaatacgg
    21661 cgaaggtctg gcgcgagcgg tgcaggacgg cctgaagaaa ggcaatgcta acgtggtgtt
    21721 ctttgatggt atcaccgccg gggaaaaaga tttctcaacg ctggtggccc gtctgaaaaa
    21781 agagaatatc gacttcgttt actacggcgg ttatcacccg gaaatggggc aaatcctgcg
    21841 tcaggcacgc gcggcagggc tgaaaactca gtttatgggg ccggaaggtg tggctaacgt
    21901 ttcgctgtct aacattgcgg gcgaatcagc ggaagggctg ctggtgacca agccgaagaa
    21961 ctacgatcag gttccggcga acaaacccat tgttgacgcc atcaaagcga aaaaacagga
    22021 cccaagcggc gcattcgttt ggaccaccta cgccgcgctg caatctttgc aggcgggcct
    22081 gaatcagtct gacgatccgg ctgagatcgc caaatacctg aaagcgaact ccgtggatac
    22141 cgtgatgggt ccgctgacgt gggatgagaa aggtgatctg aaaggctttg agttcggcgt
    22201 atttgactgg cacgccaacg gcacggcgac cgatgcgaag taatcattaa tcggcaactt
    22261 tgggttgccg ccaaattgct aatatcgagt acgttgcttc atgccggatg cggcgtaaac
    22321 gccttatccg gcctacaaga tccaaagaaa tcagtaaatt gcaacacaca ttgtaggcct
    22381 gataagcgta gcgcatcagg caatttctcg ttggtcatca cttttgaaat caacttgacg
    22441 attaacgctt ctcccagccg ccctgttgcg ccgtaaaccc cagcgcctgc ataaacgccg
    22501 tcatcacacc gcgatcttcc acgcctgcat ccgccatcca ccagcatgaa acggcaggat
    22561 tgttacgcaa aacctcttcc agcagatatt gccccacacc gcgacggcgg gtgacttccc
    22621 gcacgcgcag ggaatccagt gctccctcgg tgccgcttaa ggttacccgc acggccgcta
    22681 gcaggcgctc gttaaaacgc gcggcgtaga tgcggtggtt atcgtcaacc tgtaaagagg
    22741 aaggggaata ctccggccag atcttttgca ggtcaatccg gtcctggtcg ctaaagtttt
    22801 ctaatcgaat gatggtcagc ttcatgggta acccgtgtaa atcacaaaag tggaaccagt
    22861 gtagcgaaat aatttaatcg gaggctttct cttttttatt tcttttggca ggtgattaat
    22921 tttttaacag caataattac aaagttaaaa cattatagaa tgaaaaatac ccagcataat
    22981 cccctgaatg atagtgaatt attccgccct ttgtgccgtt attttatgct gacaaaggca
    23041 cttttttctg tttatctatc aataaattca gaatattatc tgttcttaat cgactgaaaa
    23101 atagggattt taatcactat tatcacaaaa tactgcgcta aacccttaat cagacaggca
    23161 aaaacagtgc agtataaaaa aagaacagtc tgatttgtta acacataaaa acaaagcaac
    23221 acaacatcac gaatggggat ttttgactat gaaacggaat gcgaaaacta tcatcgcagg
    23281 gatgattgca ctggcaattt cacacaccgc tatggctgac gatattaaag ttgccgttgt
    23341 cggcgcgatg tccggcccga ttgcccagtg gggcgatatg gaatttaacg gcgcgcgtca
    23401 ggcgattaaa gacattaatg ccaaaggggg aattaaaggc gataagctgg tgggcgtgga
    23461 atatgacgac gcctgcgacc cgaaacaagc cgttgcggtc gccaacaaaa tcgttaacga
    23521 cggcattaaa tacgttattg gtcatctgtg ttcttcttct acccaacctg cgtcagatat
    23581 ctacgaagac gaaggtattc tgatgatctc gccgggagcg accaacccgg agctgaccca
    23641 acgcggttat caacacatta tgcgtactgc cgggctggac tcttcccagg ggcctacggc
    23701 ggcaaaatac attcttgaga cggtgaagcc ccagcgcatc gccatcattc acgacaaaca
    23761 acagtatggc gaagggctgg cgcgttcggt gcaggacggg ctaaaagcgg ctaacgccaa
    23821 cgtcgtcttc ttcgacggta ttaccgccgg ggagaaagat ttctccgcgc tgatcgcccg
    23881 cctgaaaaaa gaaaacatcg acttcgttta ctacggcggt tactacccgg aaatggggca
    23941 gatgctgcgc caggcccgtt ccgttggcct gaaaactcag tttatggggc cggaaggtgt
    24001 gggtaacgca tcattgtcga atattgccgg tgatgctgcc gaaggcatgt tggtcactat
    24061 gccaaaacgc tatgaccagg atccggcaaa ccagggcatc gttgatgcgc tgaaagcaga
    24121 caagaaagat ccgtccgggc cttatgtctg gatcacctac gcggcggtgc aatctctggc
    24181 gactgccctt gagcgtactg gcagcgatga gccgctggcg ctggtgaaag atttaaaagc
    24241 taacggtgca aacaccgtga ttgggccgct gaactgggat gaaaaaggcg atcttaaggg
    24301 atttgatttt ggtgtcttcc agtggcacgc cgacggttca tccacggcag ccaagtgatc
    24361 atcccaccgc ccgtaaaatg cgggcgggtt tagaaaggtt accttatgtc tgagcagttt
    24421 ttgtatttct tgcagcagat gtttaacggc gtcacgctgg gcagtaccta cgcgctgata
    24481 gccatcggct acaccatggt ttacggcatt atcggcatga tcaacttcgc ccacggcgag
    24541 gtttatatga ttggcagcta cgtctcattt atgatcatcg ccgcgctgat gatgatgggc
    24601 attgataccg gctggctgct ggtggcggcg ggattcgtcg gcgcaatcgt cattgccagc
    24661 gcctacggct ggagtatcga acgggtagcc tatcgcccgg tgcgtaactc taagcgcctg
    24721 attgcgctca tctcggcaat cggcatgtcc atcttcctgc aaaactacgt tagcctgacc
    24781 gaaggttcgc gtgacgtggc gctgcccagt ctgtttaacg ggcagtgggt ggtgggccat
    24841 agcgaaaact tctctgcctc tattactacc atgcaggcgg tgatttggat tgttaccttc
    24901 ctcgccatgc tggcgctgac gattttcatt cgctattccc gtatggggcg cgcctgtcgt
    24961 gcctgcgcgg aagatctgaa aatggcgagc ctgcttggca tcaacaccga ccgggtgatt
    25021 gcgctgacct ttgtgattgg cgcggcgatg gcggcggtgg cgggtgtgct gctcggtcag
    25081 ttctacggtg tgattaaccc ctacatcggc tttatggccg ggatgaaagc ctttaccgcg
    25141 gcggtgctcg gcgggattgg cagcattccg ggggcgatga ttggcgggct gatcctgggg
    25201 attgcggagg cgctctcttc tgcctatctg agtacggaat ataaagatgt ggtctcattt
    25261 gccctgctga tcctggtact gctggtgatg ccgaccggta ttctggggcg cccggaggta
    25321 gagaaagtat gaaaccgatg catattgcaa tggcgctgct ctctgccgcg atgttcttta
    25381 tcctcgctgg cgtctttatg ggcgtacagc tggagctgga cggcaccaaa ctggtggtcg
    25441 acacggcttc ggatgtccgt tggcagtggg tgtttatcgg cacggcggtg gtctttttct
    25501 tccagatttt gcgacctgct ttccagaaag ggttgaaaag cgtttccggg ccgaagttta
    25561 ttctgcccgc gattgatggc tccacggtga agcagaaact gttcctcgtg gcgctgttgg
    25621 tgcttgcggt ggcgtggccg tttatggttt cacgcgggac ggtggatatc gccaccctga
    25681 ccatgatcta cattatcctc ggcctcgggc tgaacgtggt ggtagggctt tccggcctgc
    25741 tggtgctggg gtacggcggt ttttacgcca tcggcgctta cacttttgcg ctgctcaatc
    25801 actattacgg cttgggcttc tggacctgtc tgccgattgc cgggttaatg gcagcggcgg
    25861 cgggcttcct gctcggcttt ccggtgctgc gtctgcgtgg tgattatctg gcgatcgtta
    25921 ccctcggctt cggcgaaatt gtgcgcatat tgctgctcaa taacaccgaa attaccggcg
    25981 gcccgaacgg aatcagtcag atcccgaaac cgactttctt cggccttgag ttcagccgta
    26041 ccgcccgtga aggcggttgg gacacgttca gtaatttctt tggcctgaaa tacgatccct
    26101 ccgatcgcgt catcttcctg tatctggtgg cgttgctgct ggtggtgcta agcctgtttg
    26161 tcattaaccg cctgctgcgg atgccgctgg ggcgtgcgtg ggaagcgttg cgtgaagatg
    26221 agatcgcctg ccgttcgctg ggcttaagcc cgcgtcgtat caagctgacc gcttttacca
    26281 tcagtgccgc gtttgccggt tttgccggaa cgctgtttgc tgcgcgccag ggctttgtca
    26341 gcccggaatc cttcaccttt gctgaatcgg cgtttgtact ggcgatagtg gtgctcggcg
    26401 gtatgggatc gcagtttgcg gtgattctgg cggcaatttt gctggtggtg tcgcgcgagt
    26461 tgatgcgtga tttcaacgaa tacagcatgt taatgctcgg tggtttgatg gtgctgatga
    26521 tgatctggcg tccgcagggc ttgctgccca tgacgcgccc gcaactgaag ctgaaaaacg
    26581 gcgcagcgaa aggagagcag gcatgagtca gccattatta tctgtgaacg gcctgatgat
    26641 gcgcttcggc ggcctgctgg cggtgaacaa cgtcaatctt gaactgtacc cgcaggagat
    26701 cgtctcgtta atcggcccta acggtgccgg aaaaaccacg gtttttaact gcctgaccgg
    26761 tttctacaaa cccaccggcg gcaccatttt actgcgcgat cagcatcttg aaggtttacc
    26821 ggggcagcaa attgcccgca tgggcgtggt gcgcaccttc cagcatgtgc gtctgttccg
    26881 tgaaatgacg gtaattgaaa acctgctggt ggcgcagcat cagcaactga aaaccgggct
    26941 gttctctggc ctgttgaaaa cgccttcttt ccgtcgcgcc cagagcgaag cgctcgaccg
    27001 cgccgccacc tggcttgagc gcattggttt gctggaacac gccaaccgtc aggcgagtaa
    27061 cctggcctat ggtgaccaac gccgtctgga gattgcccgc tgcatggtga cgcagccgga
    27121 gattttaatg ctcgacgaac cggcggcagg tcttaaccca aaagaaacca aagagctgga
    27181 tgagctgatt gccgaactgc gcaatcatca caacaccact atcttgttga ttgaacacga
    27241 tatgaagctg gtgatgggga tttcagaccg aatttatgtg gtcaatcagg ggacgccact
    27301 ggcaaacggt acgccggagc aaatccgtaa taacccggac gtgatccgtg cctatttagg
    27361 tgaggcataa gatggaaaaa gtcatgttgt cctttgacaa agtcagcgcc cactacggca
    27421 aaatccaggc gctgcatgag gtgagcctgc atatcaatca gggcgagatt gtcaccctca
    27481 ttggcgcgaa cggggcgggg aaaaccacct tgctcggcac gttatgcggc gatccgcgcg
    27541 ctaccagcgg gcgaattgtg tttgatgata aagacattac cgactggcag acggcgaaaa
    27601 tcatgcgcga agcggtagcg attgtcccgg aagggcgtcg cgtcttctcg cggatgacgg
    27661 tggaagagaa cctggcgatg ggcggctttt ttgccgaacg cgaccagttc caggagcgca
    27721 taaagtgggt gtatgaactg tttccacgtc tgcatgagcg ccgtgttcag cgggcaggca
    27781 ccatgtccgg cggtgagcaa caaatgctgg cgattggtcg cgcgctgatg agcaacccgc
    27841 gtttgctgct acttgatgag ccatcgctcg gtcttgcgcc gattatcatc cagcaaattt
    27901 tcgacaccat cgagcagctg cgcgagcagg ggatgactat cttcctcgtc gagcagaacg
    27961 ccaaccaggc gctaaagctg gcggatcgcg gctacgtgct ggaaaacggc catgtggtgc
    28021 tctccgatac cggcgacgcg ctgctggcga acgaagcagt gagaagtgcg tatttaggcg
    28081 ggtaataaca cgttgattga taaggagtca aaagactcct ttgagacagg tgacaaatgt
    28141 aaaattgcct gatgcgctgc gcttatcagg cctactgggt gagttgcaat atattgaatt
    28201 tgcacgatct tgtaggccgg ataaggcgtt tacgccgcat ccggcatgaa acgatgagta
    28261 atctgtagag tttgattcag accttctatt ttccgcttat ccgtgcccca tctcccattt
    28321 tccctcaccc acgccgtcac cgccttgtca tctttctgac accttactat cttacaaatg
    28381 taacaaaaaa gttatttttc tgtaattcga gcatgtcatg ttaccccgcg agcataaaac
    28441 gcgtgaattc gcgcattcgg tacaacaaga gagataaacg atgaaaccgt tacattatac
    28501 agcttcagca ctggcgctcg gactggcgtt aatggggaat gcacaggcag tgacgaccat
    28561 tccgttctgg cattctatgg aaggggaact gggtaaagag gtggattctc tggcccaacg
    28621 ttttaacgcc gaaaatccgg attacaaaat tgtaccgacc tataaaggca actacgaaca
    28681 gaatttaagc gcggggattg ccgcatttcg taccggcaac gctccggcta ttttgcaggt
    28741 ttatgaagtt ggcaccgcca ccatgatggc atcgaaagcc attaaaccgg tatatgacgt
    28801 gtttaaagag gcggggattc aattcgatga gtcgcagttt gtgccgacgg tttccggtta
    28861 ctactccgac agcaaaacgg gccacttact ctcccagcca ttcaacagct cgacccccgt
    28921 tctctattac aacaaagacg ccttcaagaa agcaggatta gacccggaac aaccgccgaa
    28981 aacctggcag gatctggcgg actatgccgc gaaactgaaa gcctccggta tgaagtgcgg
    29041 ctacgccagc ggctggcagg gctggatcca actggaaaac tttagcgcct ggaacggtct
    29101 gccgtttgcc agcaaaaaca acggctttga cggcacggac gcggtgctgg agttcaacaa
    29161 gccggagcag gtgaaacaca tcgccatgct cgaggagatg aacaagaagg gcgacttcag
    29221 ctacgtcggt cgtaaggatg aatccaccga gaagttctat aacggtgatt gcgcgatgac
    29281 caccgcctct tccggttctc tcgctaatat tcgtgagtac gccaaattta actatggcgt
    29341 aggcatgatg ccttacgacg ccgatgcgaa agatgcaccg caaaacgcca ttatcggcgg
    29401 ggccagcctg tgggtgatgc agggcaaaaa taaagaaacg tataccggcg tggcgaagtt
    29461 cctcgacttc ctcgcgaagc cagaaaacgc tgccgagtgg catcagaaaa ccggttatct
    29521 gccaatcacc aaagcagcgt atgacctgac ccgtgagcag ggcttttacg agaaaaaccc
    29581 aggggcggat accgcgacgc gtcagatgct gaataagccg ccgttgccgt tcaccaaagg
    29641 gctgcgtctg ggcaacatgc cgcagatccg cgtgattgtg gatgaagagc tggagagcgt
    29701 gtggaccggt aagaagacac cacagcaggc actggatacc gccgttgagc gtggaaatca
    29761 gttgctgcgc cgctttgaga aatcgacgaa gtcttaatca gtgtaatgtc ggatgcgttt
    29821 cgcttatctg acctggcatc gcgtgtaggc cggataagcg aagcgcatcc ggcacagttc
    29881 aggaattaac cgtaatgtca tcatcccgtc cggtgttccg ctcgcgctgg ctgccttatc
    29941 tgctggtcgc gccgcagctc atcatcaccg ttatcttttt tatctggcct gcgggcgaag
    30001 cgttgtggta ctcgctacaa agcgtcgatc cgtttggttt ctccagccag tttgtcggcc
    30061 tggataactt cgtcacgctg ttccatgaca gctactatct cgactccttc tggacgacga
    30121 taaaattcag cacctttgtc accgtcagcg gtttgctggt atcgctgttc tttgcggcgc
    30181 tggtggagta catcgtgcgc ggcagccgtt tctatcaaac cttaatgttg ctgccttacg
    30241 ccgtggctcc cgccgttgcc gccgtattgt ggatcttcct gtttaacccc ggtcgcgggc
    30301 tgatcaccca ttttctcgcg gagttcggct acgactggaa ccacgcgcaa aacagcggtc
    30361 aggcaatgtt tctggtggtg tttgcctcag tatggaagca aatcagctac aacttcctgt
    30421 tcttctatgc cgcgctgcaa tccattcccc gttcgttgat cgaagccgca gccatcgacg
    30481 gtgcagggcc gattcgccgc ttctttaaga ttgcgctgcc gcttatcgcc ccggtgagtt
    30541 tcttcctgct ggtagtgaac ctggtgtatg ccttcttcga caccttcccg gtgatcgacg
    30601 ccgccacgtc cggcgggccg gttcaggcca ccacgacgct gatttataag atctaccgcg
    30661 aaggttttac cggactggat ctggcttcgt ctgccgcaca gtcggtggtg ttgatgttcc
    30721 tcgtcatcgt gctgacggtg gtgcagttcc gctatgttga aagcaaggtg cgttaccaat
    30781 gattgagaac cgtccgtggc tgacgatatt cagccatacc atgctgatcc tcgggatcgc
    30841 ggtgatcctc ttcccgctgt acgtggcgtt tgtcgcggcg acgctggata aacaggccgt
    30901 ctatgccgcg ccgatgacgc tcatccccgg cacacatctg ctggaaaaca tccacaacat
    30961 ctgggtgaac ggggtaggca cgaatagcgc gccgttctgg cggatgttgc ttaacagctt
    31021 tgtgatggcg ttcagcatta cgctcggcaa aattaccgtc tcgatgctct cggcatttgc
    31081 cattgtctgg tttcgttttc cgctacgtaa cctcttcttc tggatgattt ttatcaccct
    31141 gatgctgccg gttgaagtac gtatcttccc gacggtggaa gtcatcgcca acctgcagat
    31201 gctcgacagc tacgccggtt taacgctgcc gctgatggcc tcggcgaccg ctactttcct
    31261 gttccgccag ttctttatga cgctgccgga tgagctggtg gaagccgcgc ggatcgacgg
    31321 cgcatcgcca atgcgcttct tttgcgacat cgtttttccg ctctccaaaa ctaatctggc
    31381 ggcgctgttt gtgatcacct ttatctacgg ctggaatcag tatttgtggc cgttgttgat
    31441 tattaccgat gtggatctcg gcaccaccgt ggcagggatc aaagggatga tcgctacagg
    31501 cgaaggcacc acggaatgga actcagtgat ggcggcgatg ttgttaacgc ttatccctcc
    31561 ggtggtgatt gttttagtga tgcagcgtgc cttcgtgcgc ggcctggtcg atagtgagaa
    31621 ataagatggc aggactgaaa ttacaggcag taaccaaaag ctgggatggt aaaacccagg
    31681 tcattaaacc gctgaccctt gatgtggcgg atggcgaatt tatcgtgatg gtcgggccgt
    31741 ctggctgcgg gaaatcgacg ctgctgcgca tggttgccgg gctggagcgg gtgacagaag
    31801 gcgatatctg gatcaacgac cagcgcgtga ctgaaatgga gccaaaagat cgcgggattg
    31861 cgatggtgtt ccagaactac gcgctttatc cgcatatgag tgtcgaagaa aacatggcgt
    31921 gggggctgaa aattcgcggc atgggcaagc agcaaattgc cgagcgcgtt aaagaagcgg
    31981 cgcgcattct ggagctggac ggtctgctca aatgtcgccc gcgcgagctt tccggcggtc
    32041 agcgccagcg tgtggcgatg ggccgcgcga ttgtgcgcga tccggcggtg ttcctgtttg
    32101 atgagccgct ctctaacctc gatgccaagc tgcgcgtgca gatgcgtctt gaactgcaac
    32161 agttgcaccg tcgcctgaaa acgacttcac tctacgttac tcacgatcag gttgaagcga
    32221 tgacgctcgc ccagcgagta atggtgatga acggcggcgt tgccgaacag attggcacac
    32281 cagttgaagt ctacgaaaag cccgccagcc tgtttgtagc gagttttatc ggcagtccgg
    32341 cgatgaacct gctgacaggc cgcgtgaata ccgaaggcac gcatttcgaa ctggacggcg
    32401 gtatcgtgct accgctaaac ggtggctacc gtcagtatgc cgggcgtaaa atgactctcg
    32461 gcattcgccc ggaacatatt gcgctaagct cgcaagcaga aggtggcgta ccgctggtga
    32521 tggatacgct ggagatcctc ggcgcagata acctggcgca cggacgctgg ggcgagcaga
    32581 agctggtggt gcgactggcg catcaggagc gcccgacggc aggcagcacg ctgtggctgc
    32641 atctggcgga aaatcagctg catctttttg atggtgaaac aggacaacga gtatgagtaa
    32701 ctggccttat ccccgcatcg tcgctcatcg tggcggcggt aagctggccc ccgaaaacac
    32761 cctggcggca atcgacgttg gggcaaaata cggtcataag atgatcgaat ttgacgcgaa
    32821 gttatcgaaa gatggcgaga tcttcctgct ccatgacgac aatctcgaac gcaccagcaa
    32881 cggctggggc gtcgcgggtg aactgaactg gcaggattta ctgcgcgtgg atgcgggcag
    32941 ttggtacagc aaagcgttta aaggtgagcc gctgccgttg ctttcgcagg tggcggaacg
    33001 ctgtcgcgaa cacgggatga tggcgaatat cgaaatcaaa cccaccactg gcactggacc
    33061 attaacgggc aaaatggtgg cgctggcggc acgccaactg tgggccggta tgacgccgcc
    33121 gctgctgtca tcgtttgaga ttgatgcttt agaagctgca cagctggcgg caccggaact
    33181 gccgcgtggt ttattgcttg atgagtggcg cgacgactgg cgcgaactga ccgcgcggct
    33241 gggctgcgtc tctattcatc tcaatcataa gttactcgat aaagtgcgag tgatgcagtt
    33301 gaaagacgcc ggactgcgga ttctggttta taccgtcaat aaaccccagc gcgcggcaga
    33361 gttgctgcgc tggggcgtgg attgcatctg taccgatgcg attgacgtga ttggtccgaa
    33421 ctttacggcc caatagtttt caacggaatg tcaggctgcg gcgtgctggg ctgattcagc
    33481 atgtcgccgt ttctcctctc cggcagcata tgctgctgat tcaacgaact atccggatta
    33541 cggttgctgt ttaacatccc gccgttggtg ttgggcagca tttgctgccg ggcgggattt
    33601 cgctcccccg gctgtgactg caacacccgc tgagaattat tgtttatctg gttttctaaa
    33661 tgctgctgtt gcaactgagt ttgcgttttc agttgctgat tcagcatccc tttttgctgg
    33721 atttgctgag tctgcatctg ggtttgcatc cgctgctggc tgggtatctg ataccccggc
    33781 tggttagggt tgttcagagt attaatgggc tgtgcaaagc cgacaaacgg caggagtgcc
    33841 gtaagaatca gaagtcgttt catcgcgtat cctcctctga agatatcctt taagtttact
    33901 cgcttcccga caaaacgatg attaattcag agttatatac caggcttagc tggggttgcc
    33961 ccttaatctc tggagaataa cgatgataaa accgacgttt ttacgccggg tggccattgc
    34021 tgctctgctc tcaggaagtt gttttagcgc cgccgccgcg cctcctgcgc cgcccgtctc
    34081 gtatggtgtg gaggaagatg tcttccaccc ggtacgcgcg aaacagggaa tggtagcgtc
    34141 tgtggacgcc actgccactc aggtgggggt ggatattctc aaggagggcg ggaatgccgt
    34201 tgatgccgcc gtggcggtgg gctacgcgct ggcggtaacg catccgcagg cagggaatct
    34261 gggcggtggt ggttttatgt taatccgctc gaaaaatggc aataccacgg ctatcaattt
    34321 ccgcgaaatg gcacccgcca aagcgacccg cgatatgttc ctcgatgatc agggcaaccc
    34381 ggacagcaaa aaatcactca cttcgcatct ggcttccggc acaccgggta cggtagcagg
    34441 tttctcgctg gcgctggata aatacggcac catgccgctg aacaaagtcg tgcagcccgc
    34501 gtttaaactg gcacgcgatg gttttatcgt taacgacgcg ctggctgacg atctcaaaac
    34561 ctacggtagc gaagtgttgc cgaatcacga aaacagtaaa gctatcttct ggaaagaggg
    34621 cgagccgctg aaaaagggcg acacgctggt gcaggcgaac ctggcaaaga gcctggagat
    34681 gattgctgaa aacggcccgg acgaattcta taaaggcacg attgcggaac agatcgccca
    34741 ggagatgcag aaaaacggtg gcttgatcac taaagaagat ttagcggcct ataaagcggt
    34801 cgaacgcact ccgataagcg gcgattatcg cgggtatcag gtttactcca tgccaccgcc
    34861 atcctccggc gggatccata tcgtacaaat cctcaatatt ctggaaaact tcgatatgaa
    34921 gaaatacggc tttggcagcg ccgatgcgat gcaaatcatg gcagaggctg agaaatatgc
    34981 ctacgccgac cgctcggaat atcttggcga cccggatttt gtcaaagtcc cgtggcaggc
    35041 gctgaccaat aaagcctatg ccaaatctat tgccgagcaa atcgatatca ataaagcgaa
    35101 accgtccagc gagattcgcc ctggcaagct tgcgccttat gagagtaatc aaactaccca
    35161 ttactcagtg gtggataaag atggtaacgc ggtggcggtg acctatacgc tgaacaccac
    35221 cttcggtacg ggtattgtcg cgggcgagag cggtattctg cttaataacc agatggatga
    35281 tttctccgcc aaaccgggcg taccgaacgt ttacgggctg gtgggcggtg atgccaacgc
    35341 ggtcgggccg aataaacgcc cgctgtcgtc gatgtcacca accattgtgg tgaaagacgg
    35401 taaaacctgg ctggttaccg gtagccctgg cgggagtcgg attattacca ccgtgctgca
    35461 aatggtggtg aatagcatcg attatggcat gaatgtcgct gaagcgacca atgcaccgcg
    35521 cttccaccat caatggttgc cggacgagct gcgtgtcgaa aaagggttta gcccggatac
    35581 gctcaagctg ctggaagcaa aaggtcagaa agtggcgctg aaagaggcga tgggcagtac
    35641 acaaagcatt atggttgggc cggacggtga gttgtacggc gcatccgacc cgcgctcggt
    35701 ggatgattta acggcggggt actaaggtta gcggccctct tcgtgggaag agggctattt
    35761 tgtcagggca agccgaaggt agcctttttt atttcgtaat cctgtagata ttcttccagc
    35821 ggctttgccg ttcccagaga atgaatttca ccactgtctt tatcaataat aaaaggtgcg
    35881 ttaccagcta agcgcgcggc ctcatctcca gtttcgagaa attctcgtgc ttcgaaacag
    35941 aaataccagc cctggctaaa gcgtccatgt agagtaatga cgaccgggag atctgcatca
    36001 tcaaggtaat ggttcgcttt cgcg
//
LOCUS       DAZFXT010000041        35547 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.041, whole genome shotgun sequence.
ACCESSION   DAZFXT010000041 DAZFXT010000000
VERSION     DAZFXT010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35547
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.041"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            133..675
                     /gene="zapC"
                     /locus_tag="ACHKW0_002103"
     CDS             133..675
                     /gene="zapC"
                     /locus_tag="ACHKW0_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HFO7474937.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(672..1781)
                     /gene="ycbX"
                     /locus_tag="ACHKW0_002104"
     CDS             complement(672..1781)
                     /gene="ycbX"
                     /locus_tag="ACHKW0_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HFO7474938.1"
                     /translation="MVTLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            2025..4133
                     /gene="rlmKL"
                     /locus_tag="ACHKW0_002105"
     CDS             2025..4133
                     /gene="rlmKL"
                     /locus_tag="ACHKW0_002105"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HFO7474939.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEDIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLLVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            4144..6051
                     /gene="uup"
                     /locus_tag="ACHKW0_002106"
     CDS             4144..6051
                     /gene="uup"
                     /locus_tag="ACHKW0_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7474940.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQSVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            6181..7434
                     /gene="pqiA"
                     /locus_tag="ACHKW0_002107"
     CDS             6181..7434
                     /gene="pqiA"
                     /locus_tag="ACHKW0_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HFO7474941.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VADLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            7439..9079
                     /gene="pqiB"
                     /locus_tag="ACHKW0_002108"
     CDS             7439..9079
                     /gene="pqiB"
                     /locus_tag="ACHKW0_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HFO7474942.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            9076..9639
                     /gene="pqiC"
                     /locus_tag="ACHKW0_002109"
     CDS             9076..9639
                     /gene="pqiC"
                     /locus_tag="ACHKW0_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HFO7474943.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQITVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            9895..10062
                     /gene="rmf"
                     /locus_tag="ACHKW0_002110"
     CDS             9895..10062
                     /gene="rmf"
                     /locus_tag="ACHKW0_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0141014 - ribosome hibernation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HFO7474944.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(10132..10650)
                     /gene="fabA"
                     /locus_tag="ACHKW0_002111"
     CDS             complement(10132..10650)
                     /gene="fabA"
                     /locus_tag="ACHKW0_002111"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008693 -
                     (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HFO7474945.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(10719..12479)
                     /gene="ycbZ"
                     /locus_tag="ACHKW0_002112"
     CDS             complement(10719..12479)
                     /gene="ycbZ"
                     /locus_tag="ACHKW0_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7474946.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            12665..13117
                     /gene="matP"
                     /locus_tag="ACHKW0_002113"
     CDS             12665..13117
                     /gene="matP"
                     /locus_tag="ACHKW0_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HFO7474947.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(13193..14233)
                     /gene="ompA"
                     /locus_tag="ACHKW0_002114"
     CDS             complement(13193..14233)
                     /gene="ompA"
                     /locus_tag="ACHKW0_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HFO7474948.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(14590..15099)
                     /gene="sulA"
                     /locus_tag="ACHKW0_002115"
     CDS             complement(14590..15099)
                     /gene="sulA"
                     /locus_tag="ACHKW0_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS-induced cell division inhibitor SulA"
                     /protein_id="HFO7474949.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            15318..15947
                     /gene="sxy"
                     /locus_tag="ACHKW0_002116"
     CDS             15318..15947
                     /gene="sxy"
                     /locus_tag="ACHKW0_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HFO7474950.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(15910..18072)
                     /gene="yccS"
                     /locus_tag="ACHKW0_002117"
     CDS             complement(15910..18072)
                     /gene="yccS"
                     /locus_tag="ACHKW0_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YccS family putative transporter"
                     /protein_id="HFO7474951.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(18082..18528)
                     /gene="yccF"
                     /locus_tag="ACHKW0_002118"
     CDS             complement(18082..18528)
                     /gene="yccF"
                     /locus_tag="ACHKW0_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HFO7474952.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            18651..20705
                     /gene="helD"
                     /locus_tag="ACHKW0_002119"
     CDS             18651..20705
                     /gene="helD"
                     /locus_tag="ACHKW0_002119"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HFO7474953.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(20737..21195)
                     /gene="mgsA"
                     /locus_tag="ACHKW0_002120"
     CDS             complement(20737..21195)
                     /gene="mgsA"
                     /locus_tag="ACHKW0_002120"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HFO7474954.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(21291..21953)
                     /gene="yccT"
                     /locus_tag="ACHKW0_002121"
     CDS             complement(21291..21953)
                     /gene="yccT"
                     /locus_tag="ACHKW0_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HFO7474955.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            22126..22539
                     /gene="yccU"
                     /locus_tag="ACHKW0_002122"
     CDS             22126..22539
                     /gene="yccU"
                     /locus_tag="ACHKW0_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HFO7474956.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(22584..22901)
                     /gene="hspQ"
                     /locus_tag="ACHKW0_002123"
     CDS             complement(22584..22901)
                     /gene="hspQ"
                     /locus_tag="ACHKW0_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HFO7474957.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(22959..24149)
                     /gene="rlmI"
                     /locus_tag="ACHKW0_002124"
     CDS             complement(22959..24149)
                     /gene="rlmI"
                     /locus_tag="ACHKW0_002124"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HFO7474958.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEDGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            24244..24522
                     /gene="yccX"
                     /locus_tag="ACHKW0_002125"
     CDS             24244..24522
                     /gene="yccX"
                     /locus_tag="ACHKW0_002125"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HFO7474959.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLVQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(24519..24848)
                     /gene="tusE"
                     /locus_tag="ACHKW0_002126"
     CDS             complement(24519..24848)
                     /gene="tusE"
                     /locus_tag="ACHKW0_002126"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HFO7474960.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAKNEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(24939..25598)
                     /gene="yccA"
                     /locus_tag="ACHKW0_002127"
     CDS             complement(24939..25598)
                     /gene="yccA"
                     /locus_tag="ACHKW0_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HFO7474961.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(26006..27025)
                     /locus_tag="ACHKW0_002128"
     CDS             complement(26006..27025)
                     /locus_tag="ACHKW0_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7474962.1"
                     /translation="MGRPRKNKKDNVLPPRVRSNGYSYVWKPEGSTRSIGLGRVRKTS
                     VAKVWQNYELEKAKLHNIMTVAKLWHMFMDSPAFTELAPRTQKDYRQHQKALLMVFGK
                     VLADNVKTEQVRIFMDKRGLESKTQANHELASLSRVYGWGYERGYVKNNPCKGVRKFS
                     LKARTVYITDEQYAAIYAEAIPQLRIAMEISYLCAARLGDVLELKWQDIMDKGIYIEQ
                     NKTGTKQIKEWSPRLRTAIQLARNVSSCTCEYVINTTKGGKVIAKTLNNWWNQAKRAA
                     EQKVGVPFGCNFHDIKAKGISDYEGSSRDKQIFSGHKTENQVLIYDRKTKITPTLDLP
                     LVVSK"
     gene            complement(27003..27245)
                     /locus_tag="ACHKW0_002129"
     CDS             complement(27003..27245)
                     /locus_tag="ACHKW0_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO7474963.1"
                     /translation="MYELTLSPAEIKEITKYERYTKQQHQLRLHGIPFVIGPKNEPIV
                     LRRDIPHGLTTMPKTSELVSAEPDFEALNNGKTKKK"
     gene            complement(27313..29784)
                     /locus_tag="ACHKW0_002130"
     CDS             complement(27313..29784)
                     /locus_tag="ACHKW0_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7474964.1"
                     /translation="MSKIFICAAIPDEQAIKEDSAVAVATAIEAGDERRARAKFHWQF
                     LEQFPAAQDCAYKFIVCEDKPGIPRPALDSWDTEYMQENRWDEESASFVPVEPESDPM
                     NVNFDKLSPEVQNAVLVKFDTCENITVDMVISAQELLQEDMATFGGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCEPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLTRA
                     RVNQQNTENAAEKTGAVTVAIRREYKQTWKTLDNELACALWPGDVDAGNIDGTIHRWA
                     TNEVIDKDREDWKRISASMRKQPEALGYDRQTIFGLVRERPIDIHKDPVALNKYISEY
                     LTTKGVFEHEETDQSSTDALQPSAAQTAPVETAESDTQKNEILVEAEPSVEREGPFYF
                     VFTDKGGEKYGRANKLSGLDKALAAGGTEISKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     SEPVKVTADEVNKIMQAANISQPDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDAE
                     DQNQPNVKQNEPEAEQNSPDTQQNGPEEQQPEPAVQQELEKVCTACGQTGGGNCPDCG
                     AVMGNATYLETFDEENQAEAQKNDPEEMEGTEHLHKENTGSDQYHASDNKTGETANPL
                     IKVNGHHEISSTSRLWHHLMIDLETMGKNPDAPIISIGAIFFDPQTGEMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAILTDEIPLDDALLQLREFIDENSGEFFVQVWG
                     NGATFDNVILRRSYERQGIPCPWRYTNDRDVRTMVALGLVMDFDARTTIPFEGERHNA
                     LNDARYQAKYVSAIWQKLLPSQADF"
     gene            complement(29878..30069)
                     /locus_tag="ACHKW0_002131"
     CDS             complement(29878..30069)
                     /locus_tag="ACHKW0_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7474965.1"
                     /translation="MNTTFALVLTVYLVSGESLELVTGLYGSMKECMAAAAEQKIPGN
                     CYPVDKTTHTNNNEIPAGL"
     gene            complement(30066..30254)
                     /gene="dicB"
                     /locus_tag="ACHKW0_002132"
     CDS             complement(30066..30254)
                     /gene="dicB"
                     /locus_tag="ACHKW0_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7474966.1"
                     /translation="MEKLSCNASTSELRFEIGVITGDKTFIEDAIKQRKLEQDLLNEV
                     CIPSMLARLDLLQKGYKQ"
     gene            30828..31013
                     /locus_tag="ACHKW0_002133"
     CDS             30828..31013
                     /locus_tag="ACHKW0_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474967.1"
                     /translation="MPNKPCPACNALSGFLEKGGYYIFNCPEHVEFHISKLDSIITKP
                     NQYQSDLLNKELNAARD"
     gene            complement(31200..31589)
                     /locus_tag="ACHKW0_002134"
     CDS             complement(31200..31589)
                     /locus_tag="ACHKW0_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474968.1"
                     /translation="MEFKDLLKEIQEIAEHALHQRLNEVELESATKKYIDNMARNVRD
                     AFTGLYSVSVTNNQNTEETAKRIASVMGFHVEEKYSKKEFWKTTKKLQSENCHLLRQS
                     LLSMRKVIQMTQDYRNCSHYLKNSELS"
     gene            complement(31601..31729)
                     /gene="ydfB"
                     /locus_tag="ACHKW0_002135"
     CDS             complement(31601..31729)
                     /gene="ydfB"
                     /locus_tag="ACHKW0_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfB"
                     /protein_id="HFO7474969.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEALSFSEFKQALSSSAKSND"
     gene            complement(31731..31886)
                     /gene="ydfA"
                     /locus_tag="ACHKW0_002136"
     CDS             complement(31731..31886)
                     /gene="ydfA"
                     /locus_tag="ACHKW0_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7474970.1"
                     /translation="MDTIDLGNNESLVYGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            complement(32163..32450)
                     /locus_tag="ACHKW0_002137"
     CDS             complement(32163..32450)
                     /locus_tag="ACHKW0_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7474971.1"
                     /translation="MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLS
                     EHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIEIVSVTHSRRQFPFSI"
     gene            complement(32450..32641)
                     /locus_tag="ACHKW0_002138"
     CDS             complement(32450..32641)
                     /locus_tag="ACHKW0_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HFO7474972.1"
                     /translation="MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDE
                     VMAEMENLIAQIAVTNKSE"
     gene            complement(32669..33070)
                     /locus_tag="ACHKW0_002139"
     CDS             complement(32669..33070)
                     /locus_tag="ACHKW0_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7474973.1"
                     /translation="MMSALEVSMYRISKLLQETGWSQAELARRIGVTQQTVQQWVSGK
                     ATPKASSLDKLVEVSGHPLHWFLLPPEECEQIFTPDTMKIGPRQRELLQAFSAFPEED
                     QEKMLQEIKDKKKSMEETIARWLAAQKSRRA"
     gene            33179..33451
                     /locus_tag="ACHKW0_002140"
     CDS             33179..33451
                     /locus_tag="ACHKW0_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7474974.1"
                     /translation="MLDSTREKIRQKYTQAEIGRYMGVAQQTVWQWFSFGVPPKQVIP
                     LCQLMKWEVTPHEIRPDIYPNPTDGLPVGCKVNTSNAPELIHENQA"
     gene            33435..33860
                     /locus_tag="ACHKW0_002141"
     CDS             33435..33860
                     /locus_tag="ACHKW0_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7474975.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAKITKAYFELGMTFPELYD
                     DSHPEAMARNTQKIFRWVEKDTPDAVKKIQALLPAIEKAMPPPLVARMRSHSSAYFRE
                     LVETKERLVKDIDDFVASAIVLFDQMNRGGPAGNTLAVH"
     gene            complement(34067..34522)
                     /locus_tag="ACHKW0_002142"
     CDS             complement(34067..34522)
                     /locus_tag="ACHKW0_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7474976.1"
                     /translation="MYFLKSLYQAHVLNVAATNRWCNSPEMLPDYRAWLRAETYLRLD
                     ILISELQKETASIHNLQGIDAVRILVSRHSALSIIEVRHLSFSELIFLLQPALESANI
                     PPEVIQYPPHVDEQLQDVPYNQRAGLTPCSEAEWDHSLLKKYQDLYNPQ"
     gene            34601..>35547
                     /locus_tag="ACHKW0_002143"
     CDS             34601..>35547
                     /locus_tag="ACHKW0_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7474977.1"
                     /translation="MRDYAKVSPRFWLGETGRELRKAGAEAQVVAFYLMTSPHANMLG
                     LYYLPVLYLAHETGLGLEGASKGLKRAVEAGFCSYDHDAEMVWVHEMAAWQVGETLKP
                     GDNRCAGVRNEYASLPENAFLSVFYDRYKTDFHLDVRRNNSRNSVRGFEGAFKGLRSQ
                     EQEQEQEKEQEQDKNTMVHGKKNTTNQAGDVQTVNPGQPAGTTPEADSAYALKADSGA
                     VQQVMTAGSEQSHQLQQPEADSAIQREADRVVPESTGQSVGRVDYPDVFEQVWREYPL
                     RAGANPKKSAFSAWKARLREGVPPETMLDGVRRYARYLAA"
BASE COUNT         8949 a         8501 c         9126 g         8971 t
ORIGIN      
        1 attttcagcc ctggttgttt tatattcatc gctgttgctt atttagacat tttgtacttt
       61 tattgttgag gttattaagc gaagcgacaa tggattgtgt tgctgcggtt ttatagggta
      121 ggggagaggc agatgcgaat taaaccagac gataactggc gttggtatta cgatgaagag
      181 cacgatcgaa tgatgctcga tttagccaat ggtatgctat ttcgctcacg ttttgcgcgc
      241 aagatgttga ctccagatgc cttttctccc gcaggctttt gcgttgacga cgccgcgctc
      301 tatttctctt ttgaagaaaa gtgccgcgat tttaatttat ctaaagaaca aaaagccgaa
      361 ctggtgctga atgcactggt agcaattcgc tatctcaaac cgcaaatgcc gaaaagctgg
      421 cattttgttt cccatggtga aatgtgggtg cccatgccgg gcgatgcggc ctgcgtttgg
      481 ttgagcgata ctcacgagca ggtcaatttg ctggtcgttg aatctggcga aaatgccgca
      541 ctatgcctgc tggcacaacc ctgcgttgtt attgcgggtc gcgcgatgca gttgggcgac
      601 gccattaaaa tcatgaacga caggctgaaa ccgcaggtta atgttgacag cttcagcctc
      661 gaacaggcag tctaacgcgc caacttaagt gcagtcttcg gtacacagct acagcaaaga
      721 atggtgccat catcgcccat tgctgatttt tttagcggcg tgacttcgcc ttctaaaagc
      781 tgaacacggc aacttccaca aatgcccgcg cggcaagaat aagggatacg aattccctga
      841 ttttctaatt gctccagcaa cacctgttgg ttatttccac gaaatgcctg tccctgccag
      901 tcaatatcta catttgcgtc cggttgttgc gtgatgttgg cggtatcatc agcggcagct
      961 gcgccgtaaa tttttgccgg agccgttgcc agaatttcca cctcatcgcc aacgcgaatc
     1021 acgccgctat tacgggcaat taaattctga ccaaaatcga catcgccgtt atcctgggca
     1081 gtgcggaaag attgcaatgt ttttaatggt tcgcctgccg gatgtttttg ccctttttct
     1141 gggctgacgg tggtgaaaat acagcggcta caaggtttaa ccacatcaaa caccacatca
     1201 ccaatgcgaa tcactttcca gctatcttct tcccaggctg acgcgccgga aaccaccaga
     1261 ttggggcgga attgctccat ttttacactg gccggacaac gttgttggag atcacgtaac
     1321 gaggcttcgt tagcaagaag gtaaggatag ccatcagcaa atgacagggg tacagtgttg
     1381 tggcgtttca cgcgccgggt catttgtggc cccacccagc gtaattgcac ttcgcgggag
     1441 aaaaatccac ttagccattt gttgatcgcg tctggcgcaa ttcgcgcggt aaaatgtgtg
     1501 ccccaaactt cggttggtgc gtcttgtgtg gcgaaatcag caaaacgaac atatgcgcta
     1561 ctgccatctg gtgcggtgag atgcaagcca tcatgcacag gtgaaggagt aaaccgtacc
     1621 atctggggaa actggcgagc ggtaataaac gtaccgtcag gttccgtgat cataaagatg
     1681 cgatcgaagg ccagaccact gacatcagcc agagcatgtg taagaccaat gccgcgcatc
     1741 gatttaacag gatgaataaa aagccgggtt aatgtcacca cggtgcggtc ctcaaatgaa
     1801 aataagccct caactttatg acatgacgcg cttattagct ataatgcgca acaattttct
     1861 tagcgcctgt cccattaggc tattttattt gtcagtttgg ccctgggttg tgctcgaaat
     1921 cctcacgtac ttgtgtacgc tccggtttct ccgcgctgtc catgtccaga ctgactgcaa
     1981 caattacgcc tactgcggta ggctcttaga gtaaaagtga cgatatgaat tctctgtttg
     2041 ccagtacggc ccgtgggctg gaagagctgt taaaaactga actggaaaac ctgggggccg
     2101 ttgaatgcca ggtggttcag ggtggggtcc atttcaaggg cgacacacgg cttgtttacc
     2161 agagcctgat gtggagccgc ctggcctcgc gtattatgtt gccgctgggc gagtgtaagg
     2221 tttacagcga tttagacctc tatctcggtg ttcaggcgat caactggaca gagatgttta
     2281 atcctggcgc gaccttcgct gtccacttca gtggtttgaa tgacaccatc cgcaacagtc
     2341 agtacggcgc gatgaaagtg aaagacgcga tcgtcgatgc tttcacgcgg aaaaatctgc
     2401 cgcgtccaaa tgttgatcgc gatgcgccgg atatccgcgt taacgtctgg ctgcataaag
     2461 aaaccgccag tatcgctctt gatctgagtg gtgatggttt acatctgcgt ggctatcgcg
     2521 atcgtgctgg tattgcgccg atcaaagaaa ccctggcagc cgctattgtg atgcgatccg
     2581 gctggcagcc aggaacaccg ctgctcgatc cgatgtgtgg ttccggtacg ttgctgattg
     2641 aagcggcgat gctagcgacc gatcgcgcac caggcttgca ccgtgggcgt tggggcttta
     2701 gcggctgggt gcagcatgat gaagatatct ggcaggaagt gaaagcggaa gcgcaaactc
     2761 gtgcccgtaa aggcctggct gagtatagct ctcattttta cggttcggac agcgacgcac
     2821 gggtgattca acgtgcgcgc actaacgccc gtcttgcggg gattggtgaa ctgatcacct
     2881 ttgaggtgaa agatgtcgcg caactgacca atccgctgcc gaaagggccg tacggcacag
     2941 tgttgagcaa cccgccatac ggggaacgtc tggacagcga accggcgctg attgcgctgc
     3001 atagcctgct gggtcggatc atgaaaaacc agtttggtgg ctggaatctc tctttgttta
     3061 gtgcctcgcc ggatctgcta agctgcttgc agctgcgtgc agacaaacag tacaaggcga
     3121 aaaacggccc gctggactgc gtacagaaaa attaccacgt tgccgaaagc actccagaca
     3181 gcaaaccggc gatggtagcg gaagactacg ccaaccgcct gcgtaagaac ctcaaaaaat
     3241 tcgagaagtg ggctcgccag gaaggaattg aatgttaccg cctgtatgac gccgatctac
     3301 cggaatataa cgttgccgtt gaccgttatg ccgactgggt ggtggtgcag gagtatgcgc
     3361 cgccaaaaac tattgatgct cacaaagcgc gtcagcgtct gttcgatatt atcgctgcaa
     3421 ccatttcggt actggggatt gcgccaaaca aactggtgct gaaaacccgt gaacgccaga
     3481 agggcaaaaa tcagtaccag aaactgggcg agaagggcga gtttcttgaa gttaccgaat
     3541 ataacgctca cttgttggtg aacctgacgg attacctcga taccggtctg tttctcgatc
     3601 accgtatcgc ccgtcgtatg ctcggtcaga tgagcaaagg caaagatttc ctcaacctgt
     3661 tctcttatac cggcagcgcc accgtgcatg cgggattagg cggtgcacgc agcaccacca
     3721 ccgtggatat gtcgcgtact tatctggagt gggcagaacg caacctgcgt ttgaatggcc
     3781 tgaccgggcg tgcgcatcgc ctgattcagg ccgattgcct ggcgtggctg cgtgaggcaa
     3841 atgaacagtt cgatctgatc tttatcgatc cgccaacctt ctctaactca aaacgaatgg
     3901 aagatgcgtt tgatgttcag cgcgatcatc tggcgctgat gaaagatttg aaacgtctgc
     3961 tgcgtgcagg tgggacgatt atgttctcga acaataaacg tggcttccgt atggatctcg
     4021 acggcctggc aaaactggga ctgaaagcac aagaaattac gcaaaaaacg ctctcccagg
     4081 atttcgcccg taaccgccag atccacaact gctggctgat taccgcagcc taaaggaata
     4141 gtaatgtcat taatcagtat gcatggcgca tggctgtcgt tcagcgacgc gccgcttctc
     4201 gataacgcag aactgcatat cgaagataac gaacgtgttt gtctggtggg ccgcaacggc
     4261 gcgggcaaat cgacgttaat gaaaatcctc aaccgtgaac aagggctgga tgacggtcgc
     4321 attatttacg agcaagattt gattgtagct cgtctgcaac aggatccgcc gcgtaacgtt
     4381 gagggtagcg tttatgattt cgttgccgaa ggcattgaag aacaagcgga atatctgaaa
     4441 cgctatcacg atatttcgcg cctggtgatg aacgacccga gcgagaaaaa tctcaacgaa
     4501 ctggcgaagg ttcaggaaca gctggatcac cacaacctgt ggcagctgga aaaccgcatc
     4561 aacgaagtgc tggcgcaact ggggttagat cctaacgttg cgctgtcgtc gctttccggc
     4621 ggctggttgc gtaaagcggc attaggacgc gcgctggtga gtaatccgcg cgtgctgttg
     4681 cttgatgaac cgacaaacca cctggatatt gaaaccatcg actggctgga agggtttttg
     4741 aaaactttca acgggacgat tattttcatc tcccacgacc gttcgtttat ccgcaatatg
     4801 gcgacgcgca ttgttgatct cgatcgcggc aagctggtga cctatccagg gaattacgac
     4861 cagtacctgc tggaaaaaga agaagccctg cgcgtggaag aattacaaaa tgccgagttc
     4921 gatcgcaaac tggcgcagga agaggtgtgg atccgccagg ggatcaaagc acgccgtacc
     4981 cgtaatgaag gccgcgtacg cgccctgaaa gcgatgcgtc gcgagcgtgg tgaacgtcgc
     5041 gaagtgatgg gtaccgcaaa gatgcaggtg gaagaggcca gccgctccgg taagatcgtt
     5101 ttcgaaatgg aagacgtttg ctaccaggtt gacggtaagc aactggtgaa agatttttcg
     5161 gcccaggttc tacgtggcga caaaattgcc ctgattggtc cgaatgggtg cggcaaaacc
     5221 acgctgctga aactgatgct cggtcagctt caagcggaca gcgggcgtat tcacgttggc
     5281 accaaactgg aagtggctta tttcgatcag caccgcgcgg aactggatcc cgataaaacg
     5341 gtgatggata accttgccga aggtaagcaa gaggtgatgg ttaacggcaa gccacgccac
     5401 gtattgggct atttgcagga ctttctgttc catccgaaac gggcgatgac gccggtacgt
     5461 gcgctttctg gcggtgagcg gaaccgcttg ctgctggcgc gtttgttcct caaaccaagc
     5521 aacttattga ttcttgacga accgaccaac gatcttgatg tcgaaacgct ggaactgctg
     5581 gaagaactga tcgacagcta tcagggcacg gtattgctgg ttagccacga tcgtcagttt
     5641 gtcgataaca ccgttacaga atgttggatc ttcgaaggcg gcggtaaaat tggtcgttat
     5701 gtcggcggtt atcatgatgc ccgtggtcag caagagcagt ctgtggcgct caaacagcct
     5761 gcggtgaaaa aaaccgaaga agccgccgcg gcaaaagcag aaactgtaaa acgcagcagt
     5821 agcaaactaa gctataaatt gcagcgcgaa ctggagcagc taccgcaatt gctcgaagat
     5881 ctggaggcga agctggaagc cctacagacg caggtggcgg atgcttcctt cttcagtcag
     5941 ccacatgagc agacacaaaa agtgctcgcc gatatggctg ctgcagagca ggagctggag
     6001 caagcctttg aacgctggga gtatcttgaa gcgttaaaaa atggtggctg atcgcaaaat
     6061 agtcgattta aacagagaca gctgacaacg gtaaaattgc ctgatgaact gggtttatca
     6121 ggcatgaaaa ccaacacttt gttagtcatc tgtaaaatag cgcatcatta aggagtacca
     6181 atgtgcgaac atcatcatgc cgcgaagcac atcctgtgct cgcagtgtga catgctggtg
     6241 gcgttaccgc gccttgagca tggtcagaaa gcggcatgtc cccggtgtgg cacaacgtta
     6301 accgtggcgt gggatgcccc tcggcagcgt cccaccgcct atgcgttggc tgcactgttc
     6361 atgctgttgc tgtccaactt gtttcctttt gtgaatatga acgttgcagg agttaccagt
     6421 gaaattacat tactggaaat tcccggcgtg cttttttctg aggactacgc cagcctcggc
     6481 acctttttcc tgttgtttgt gcaactggtt cccgcgtttt gtctgataac cattctgtta
     6541 ctggtgaatc gcgcggaatt accggtccgt ttaaaagagc aactggcacg ggtgcttttt
     6601 caactcaaaa cctggggaat ggcggagatt ttcctcgctg gtgtgctggt cagtttcgtt
     6661 aaactgatgg cttacggcag cattggggta ggcagcagct ttctcccctg gtgtttattt
     6721 tgtgtcctgc aactgcgcgc ttttcagtgc gttgatcgtc gctggttatg ggacgacatc
     6781 gccccgatgc cagaactgcg ccagccgcta aaaccaggcg tcacggggat acgtcagggg
     6841 ctgcgttcgt gctcctgttg tacggcaatc cttcctgctg atgaacccgt gtgcccgcgt
     6901 tgtagtacca aagggtacgt tcggcgtaga aacagcctgc agtggacact cgcgctgctt
     6961 gtaacgtcca tcatgctgta tcttccggct aatattttgc ccatcatggt ggcggattta
     7021 ttaggctcga agatgccgtc gacgattctc gctggggtca ttctgttatg gagcgaaggc
     7081 tcttatcccg tcgctgcggt gatctttctg gccagtatta tggtgccaac gttaaagatg
     7141 atcgccatcg cgtggctgtg ttgggatgcc aaagggcatg gcaagcgcga cagtgaaaga
     7201 atgcatttga tttatgaagt tgttgagttt gtaggccgct ggtcgatgat tgacgttttc
     7261 gttatcgcgg tgctctcggc gctggtgcgt atgggaggtt taatgagtat ttatccggca
     7321 atgggtgcat taatgtttgc tttagtcgtc ataatgacaa tgttttctgc tatgacgttt
     7381 gacccgcgtt tgtcgtggga tcgtcaacct gaatcagagc atgaggagtc ctgacagtat
     7441 ggaatctaat aatggggaag ccaaaatcca gaaagtgaag aactggtctc ccgtgtggat
     7501 atttcctatc gtcacggcgc tcattggggc ctgggttctt ttttatcatt acagccatca
     7561 gggaccggaa gtgaccctga tcaccgcgaa tgcggaagga attgaaggtg gcaaaaccac
     7621 cattaaaagc cgtagcgttg acgtcggcgt ggttgaaagc gccacactgg ctgatgattt
     7681 gacgcacgtt gaaatcaaag cgcggctgaa ttccggtatg gaaaaattgc tgcataaaga
     7741 caccgtcttt tgggtggtga aaccgcagat tggtcgcgaa gggattagcg gcctgggaac
     7801 gctgctgtct ggagtttata tcgaactgca gccaggcgcg aaaggcagca aaatggataa
     7861 atacgatttg ctggactcgc caccgttggc cccgcctgat gcgaaaggta tccgtgtgat
     7921 tctcgatagc aaaaaagccg ggcagctctc gccaggagat ccggtgctgt tccgtggcta
     7981 tcgggtaggt tcggttgaaa ccagcacctt cgatacacaa aaacgcaata tcagctatca
     8041 actgttcatc aatgcacctt atgaccgact ggtgaccaac aatgttcgct tctggaaaga
     8101 tagtggcatt gcggttgatc tgacgtcagc agggatgcgt gtggagatgg gctcattgac
     8161 aacgctgctg agtggcggtg tcagctttga tgtgccggaa ggtctggatt tagggcagcc
     8221 agtggcaccg aaaacagctt tcgttttgta tgatgatcag aagagcattc aggattcgtt
     8281 gtacaccgat cacattgatt atctgatgtt ctttaaagat tcggtacgcg gtctgcaacc
     8341 gggagctccg gtagagttcc ggggtattcg cctgggtacc gtaagcaaag tgccattctt
     8401 tgcgccgaat atgcgtcaga catttaacga tgattaccgt attccggtac tgattcgtat
     8461 cgagccagag cggctgaaaa tgcagcttgg cgaaaatgcg gatgttgttg agcaccttgg
     8521 cgaattgttg aaacgtggtt tacgcggatc gctgaaaacc ggaaacctgg tcactggtgc
     8581 actgtatgtt gatctcgatt tctatccaaa tacgcctgca ataaccggta ttcgtgaatt
     8641 taatggttat cagattatcc cgaccgttag cggcggcctg gcgcaaatcc agcaacgact
     8701 gatggaagcg ttggataaga tcaacaaact gccattgaat ccgatgattg aacaggcaac
     8761 cagtacgctt tctgaaagtc agcgcacaat gaaaaacctg caaacgacgc tggatagcat
     8821 gaacaagatc ctcgctagcc agtcgatgca gcagttgccg acggatatgc agtcaacgtt
     8881 gcgtgaattg aatcgcagca tgcagggctt ccagcctggc tccgcagcct acaacaagat
     8941 ggtggcggat atgcagcgcc ttgatcaggt gttgcgagaa ctgcaaccgg tgctgaaaac
     9001 gctcaatgag aagagtaacg cgctggtatt tgaagcgaag gacaaaaaag atccagagcc
     9061 gaagagggcg aaacaatgaa aaagtggcta gtgacgattg cagcactgtg gctggccgga
     9121 tgcagctccg gcgaaattaa taaaaactat taccagttac ctgtggtgca gagcggtaca
     9181 caaagtaccg ccagccaggg caatcgtctg ttatgggtag agcagatcac tgttcctgac
     9241 tatctggcgg ggaatggtgt ggtttatcaa accagtgatg tgaagtatgt gattgccaac
     9301 aacaacttgt gggccagccc gttggatcaa cagttgcgca acaccctggt tgccaacctg
     9361 agtacgcaac tgcccggctg ggtggttgcc tcccagcctc tgggaagcgc ccaggacacg
     9421 ctcaatgtta ccgtaacgga gtttaacggt cgctatgatg gcaaggtcat tgtcagtggt
     9481 gagtggctgt tgaaccacca gggacaactg atcaaacgtc cgttccgtct ggaaggagtg
     9541 caaactcagg atggttacga tgagatggtt aaagtgctgg ccggtgtctg gagtcaggaa
     9601 gccgcttcta ttgcacaaga gataaagcgt ctaccttaat tataaagatt tgtaaatata
     9661 accgtctccg gtatgttgcc tgaggcggtt tttttgtctc taacgtgcgg aaaaatttgt
     9721 tcctcttcac attttttgta caaccgacat gcccgtgtag ctcacaaata tgacagtggc
     9781 gtgaattttg cgcattgacg gcagttatga ttcgcggtat tgcttaactg tgattgcaca
     9841 tttagtaatc actgttttct tttccaccag aaaccagtat gagggaaacg aggcatgaag
     9901 agacaaaaac gagatcgcct ggaacgggca catcaacgtg gttatcaggc cggcatcgcc
     9961 ggacgctcaa aagaaatgtg tccctatcag acgctgaatc aaaggtcaca atggctggga
    10021 ggctggcgag aagccatggc ggacagggta gtaatggcct gattctgtct ctttaaaaag
    10081 aaacctccgc attgcggagg tttcgccttt tgatactctg tctgattata atcagaaggc
    10141 agacgtatcc tggaacagac cgactttcag gtcgctggcg gtatagatca gacgaccatc
    10201 aaccagcact tcgccatccg ccaggcccat aatcagacga cggttaacaa tgcgtttaaa
    10261 gtgaatacgg taggtcactt ttttcgctgt cggcagtacc tgaccagtga atttcacttc
    10321 gccaacgccc aacgcgcggc ctttaccttc gccgcccagc cagccgaggt agaaccctac
    10381 cagctgccac attgcgtcca ggcccaggca tcccggcata accggatcgc caataaagtg
    10441 gcatccgaag aaccacagat ccggattgat atccagttct gcttcaacat accctttgtc
    10501 gaagttacca cccgtttcgg tcattttgac cacacggtcc atcatcagca tgttcggtgc
    10561 tggcaattgc gggcctttag cgccaaacag ttcaccgcga ccagaggcaa gaaggtcttc
    10621 ttttgtatag gattcgcgtt tatctaccat gttctctgta agccttattt tattgaagca
    10681 cgcaggatag ctaacacgtg tacgctgaac aagtccgatc agttcggaat aaaccagttc
    10741 agccaacgta atggccatgg aaaacggtga cgtccttcct gttgcgatgc ttgcgcgata
    10801 cgttcctgga tggtttgcat cagcgtcgtt tggccttcgc catcccacac cagatttaat
    10861 aataacggca gtgcgtcagt cacatcgtct actgcccaga tggtgaattt gccttcttct
    10921 accgctttca ccagttcact gtgaagactt aaatggcgaa cgttagctgt ggggataatg
    10981 acaccttgtt tcccggttaa ctcacgttgc tggcaaatag caaagaagcc ttcgattttc
    11041 tcatttaaac caccgaccgg ctgggcgcga ccgaactgat cgactgaacc tgtgatagcg
    11101 atactctgat tcaccggcac atcggcgagg gcgcttatca gggcgcagag ttcagccatc
    11161 gaggcactat ctccatcaac ttcactgtat gactgctcaa atgtcagcga tgctgagaag
    11221 gggatctgtt gctcaagctg tagttccgac atcaggaacg cttgcatgat catcatccct
    11281 ttcgcatgga tattgccgcc aagctccgct ttgcgttcga tgtcggtgaa ttcaccatcg
    11341 ccaatatgca caacgcagct aatgcgagaa ggttcgccaa aagcgcgtgg atgacccgga
    11401 aattcaatga ccgaaagggc gttaatttgc ccgatgcgtt cgccttcggt ttcaatcagg
    11461 atttgctcct gaaggatctc atcctgcata cgttcagcga ggaaaccttc gcgccattca
    11521 cgctgctgca gcattaagtt tagctgctcg ccggagaagg tgtcgccatc acacagggag
    11581 gcgacctctt tacactggcg gaggatccac tgcgggctaa gcggaagtgt ttcttgttca
    11641 ccggtgtagc gtgctgcttc gcggataagt accggccagg catccgctcc cggtgcaggt
    11701 aagtgattat gtctggcggt aaatgtcacc cagcgacacc actgggttac tgactccgca
    11761 tcgacaatct gcagagtatc ttcaaattcg ctataaatag cctgctctga aagctctggc
    11821 tccatctcct ggaaatcagc caatgattcg cgttcgccta ccagaatgac tttcagcttc
    11881 aatggcatcg aaggcacaga gacggggaga gggcgcgact catcaaacgc aacccagtca
    11941 aaacgctcgc ggttaacgat atttttcagc cgcatccaca gcagaggttg cgccagcagt
    12001 gtacgcaaag agatgatgag aataccgcca tttgcctgat gcaccagacc aggctgcagg
    12061 gtaatgtcgc cattaaactg gcgcaggcag ccaaagagtt gctccgcttc tacccagtcg
    12121 gcagcgacaa cttgcgttaa agtcgcaaaa ttatcatctg cactcactgc gtgacgtaag
    12181 cggatggagt ggccggaaac ctcatagtga ccgcccacca gttggcctgc atcgctttgt
    12241 agcgtacgcg cggcattggc aataagattc agatactcag actcttccgg ggccttcgcc
    12301 agcataaaag aggaggatgc tcgcgtatgc agcaactgct ccagtgcgaa ttgcagtcgc
    12361 ggttgagtat cactgaataa aggatcgttt tcgtcaatca aatgtggctg agcaaatatt
    12421 tcctgatagc tatcggtatc aggaaccagg tcacgccatg caagtttcgt aatggtcaaa
    12481 gttgatgttt tttagtctgt tgtcaaagcc gcgattatac cgtaaccggc actacagcac
    12541 acgtagaaag caccgacaat actcctggca tgggcgttaa agctcacagg atggagattc
    12601 ttttcttcac tggcctaaaa agctgatatt ctgtaaagag ttacacggta acattgagat
    12661 cgctatgaaa tatcaacaac ttgaaaatct tgaaagcggt tggaaatgga agtatctggt
    12721 gaagaagcat cgcgaagggg agttaatcac ccgttacata gaagccagtg ccgcccagga
    12781 agccgtcgat gtgttgctct cgctcgaaaa tgaacccgtg ctggtaaatg gctggattga
    12841 caagcatatg aatccggaac tggtcaatcg catgaagcaa accattcggg caagacgaaa
    12901 gcgccatttt aatgcggaac accagcatac gcgcaaaaaa tcgatcgatc tggaatttat
    12961 tgtctggcaa cgtctggctg gtcttgcgca gcgtcgcggt aaaacgcttt ctgaaacgat
    13021 tgttcagctg attgaagatg cggaaaacaa agagaaatac gcgaataaaa tgtcttctct
    13081 gaagcaggat ctgcaggcat tgctgggtaa ggaataactg acgaaagtca gttcaattta
    13141 ctaaaggcaa aaaaaacccc gcagcagcgg ggtttttcta ccagacgaga acttaagctt
    13201 gcggctgagt tacaacgtct ttgatacctt taacttcgat ctctacgcga cgatccggag
    13261 ccaggcagtc gatcagtgca gcacgctgtt tcacgttgtc acaggtgttg ccagtaaccg
    13321 ggttggattc gcccatacca cgtgcggaga tcttgtctgc cgggatacct ttggagatca
    13381 ggtaatcaac aacagactga gcacggcgct cggacagacc ctggttgtaa gcgtcagaac
    13441 cgatgcggtc ggtgtaaccc agaacaacta cggaaccgtc tttcggatcc aggttgctca
    13501 gctggctgta cagctgatcc agagcagcct gaccttccgg tttcagggtt gctttgttga
    13561 agttgaacag aacgtcagac ttcagagtga agtgcttggt ctgtacttcc ggtgccggag
    13621 ctggagccgg agcaactact ggagctgctt cgccctgacc gaaacggtag gaaacaccca
    13681 ggctcagcat gccgttgtcc ggacgagtgc cgatggtgtg tgcgtcaccg atgttgttgg
    13741 tccactggta ttccagacgg gtagcgattt caggagtgat cgcgtactca acaccgccag
    13801 cgaagaccgg agaaacgccg gtgtcgtggt ttttaccata aacgttggat ttagtgtctg
    13861 cacgccatac cataccaccc agacgagtgt agatgtccag gtcgtcagtg attgggtaac
    13921 ccagtttagc ggtcagttga acgccctgag ctttgtatgc accgttttca acgctgcctt
    13981 tgtacggcat acgacctaac cagtcgtaac ccatttcaaa gccaacatac gggttaacct
    14041 ggtaaccacc aaaagcacca gcgcccagtt ggttttcatg ggtcgggcca ttgttgttga
    14101 tgaaaccagt gtcatggtac tgggaccagc ccagtttagc accagtgtac caggtgttat
    14161 ctttcggagc ggcctgcgct acggtagcga aaccagccag tgccactgca atcgcgatag
    14221 ctgtcttttt cattttttgc gcctcgttat catccaaaat acgccatgaa tatctccaac
    14281 gagataacac ggttaaatcc ttcaccgggg gatctgctca atattaactc taccgatatc
    14341 ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg aaaacttaca
    14401 agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt ttaatctttt
    14461 ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag cctccgcaag
    14521 gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca ggattaatcc
    14581 taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt gtctcgtggc
    14641 gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt catttgccgc
    14701 atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac cgatcaccac
    14761 actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc aaggggagag
    14821 ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc attcccgact
    14881 cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga gttgctgtaa
    14941 caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga caacttcact
    15001 gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg ctgcggatga
    15061 gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag cccctgtgag
    15121 ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat tttcggaaag
    15181 agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc cccttaagaa
    15241 taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt ttatgttatt
    15301 attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa tcacaagaat
    15361 acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac agcctgaccg
    15421 ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg gcttgtgagc
    15481 aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa aagtgtggcc
    15541 gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat caactgaagc
    15601 tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc acgcgcaata
    15661 cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg attctcgggg
    15721 aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg tgttggttgc
    15781 gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt gaaggtgcca
    15841 ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt gcagaatggg
    15901 ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct ggcgttgcag
    15961 acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga ccagaggttc
    16021 tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag ctaatgcctc
    16081 attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat agcaaactgc
    16141 gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag caccgagggc
    16201 tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg cttcgcgaat
    16261 ttgcggggta acgttcggtt cgctggacat atttgatacc accgacgcca gttcagcatc
    16321 acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac gcccctgatg
    16381 gtattgctcc agtatggcat cgagataccg gcagttggcc tctgtggcgc gttcgagcat
    16441 tcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg cccacgcaat
    16501 ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac cttcacccag
    16561 caagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatggg catattgcac
    16621 gttacggaag gcaaaaaaga gcacgccggt aataaccagt agcaccagct gcccttccag
    16681 tgacggtaca aaccacagca caggaatgcc aatggcgata ccgaccagcg taccaataat
    16741 ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa acaaacttgt
    16801 cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt agccgaagca
    16861 caacaccagc gacattctta ccgcatgacg gaagagggcg gattccggcg tgaagtgacg
    16921 gctaagacgc agccagatat cactcaatcc gtgcgggcta tcatcagcga gctcattttc
    16981 gtcattatta tggggcagtg cctgggcctg ttctgattca attgttgcca gttgggcatc
    17041 aatggcgcgt aaattgttca gcaaaaatcc cagtgtttta agtaaatcgg caggcgcgcc
    17101 gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct caaaatgcgg
    17161 atcatgttga taaggctgac gcagcaaaat acagcgtgac agttgctggc acgcctggcc
    17221 ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa aatgttcacg
    17281 caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct gtgcgacaaa
    17341 gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta agcgggtcag
    17401 cagcgagagt ttcgtctgat tcaatgtcgc catcagctga ccgttggcga gagccaaatc
    17461 gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg acttgagctc
    17521 aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg ggcggaccgg
    17581 gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg ccagcagata
    17641 catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt aaatggcgat
    17701 cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga gcaaaatgaa
    17761 gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa acagcaattc
    17821 tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac gcaaacgtcc
    17881 cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg ttagcggaat
    17941 cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa gcgcaataaa
    18001 aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca aaggacttag
    18061 cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt cacgcgcagc
    18121 ttgcgccgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa tagcggcaat
    18181 tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg cgatgcccgt
    18241 tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa tattcaatac
    18301 cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat cgacatgaat
    18361 agcttcattg ccataaggca ccagagacag tttagtgatc tcccagcagg atcgtgtcag
    18421 cggtaaggta aaaatcagca taatactgac cagagtcgcc aacagccagc ccagagtggt
    18481 ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa aacctcgctt
    18541 tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg agcacgtttt
    18601 ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc atggaactga
    18661 aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg gtgatcctca
    18721 atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc aatcaattac
    18781 tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta ctgccggacg
    18841 aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt taccatcatc
    18901 ttgatgctca ctggcagcgg tggagtggcg agatgagcga aattgcgtct ggtgttttac
    18961 gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg acgcgtgagc
    19021 aaacctctgg cgttcagcaa caaatccgcc aggctatgtc ggcgttgccg ttgccggtta
    19081 accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag gcctggttga
    19141 aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc atgcttaccg
    19201 agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg caggcccggg
    19261 cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc ggaaaaacgt
    19321 cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc cctgagcaaa
    19381 ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgt attcgcgaac
    19441 ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg catattattc
    19501 agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc gctgcccgtc
    19561 atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg caggcgaagg
    19621 gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt aacttctggg
    19681 atgatgaaaa attacagcgt cgtcttgcat cacgcctcga tcgttgggta agtctgatgc
    19741 ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag attcgcgatc
    19801 tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa ggtgcgctga
    19861 aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg attctggaga
    19921 aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag gatatctcgc
    19981 cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg acgttgttcg
    20041 ccgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg tcgctcacca
    20101 ccgctttcca tgaaaacttt ggtgaaggcg atcgctgtga tttagacacg acttaccgtt
    20161 ttaacagtcg tatcggtgag gtggcaaacc ggtttattca gcagaaccca ggccagttga
    20221 aaaagccgct aaacagctta accaatggag acgaaaaagc cgtcacgtta ttggatgaga
    20281 gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa gagcgcattc
    20341 ttatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg gcaacacgct
    20401 ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa caggcggatt
    20461 acgtcatcat cgttggcttg caggagggga gtgatggttt tccggctgcg gcgcgggagt
    20521 cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct gaagaacggc
    20581 ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg tttaacaaag
    20641 agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggta gcgagaaaac
    20701 cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt ccgcgagata
    20761 acgctgataa tcggggatca gaatatcgac cgcgtcgttg aaatgcggcg actggattat
    20821 gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg ccagacgcaa
    20881 caaggctttc acgtcaggat cgtgcggcac ggcatttagt gggtcccaga agaaaatcaa
    20941 tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc ccattgggcc
    21001 actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac cggtagtgcc
    21061 tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc agctcatcag
    21121 catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag gtaaagtgcg
    21181 agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac tgtaagcact
    21241 tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc tcaggaagat
    21301 ggttgttttt ccgcccattg taggaaagta tttcgcgttt gtgggtcagc cagtttaaac
    21361 caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc atccagctcg
    21421 gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg cggtagtgaa
    21481 gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat cgtttttgcc
    21541 gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc gccatccaac
    21601 aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc caggcgaggc
    21661 aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag cggtggggag
    21721 atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg aaacactaac
    21781 tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga gctggaaacc
    21841 ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt ggtggcaaaa
    21901 acggataccg gcaaacataa tgcaatcaag gtggtcacga tgccggtttt cataggtagc
    21961 tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat catacttact
    22021 aacatataga catatttccc gcttttgctc tcaatcattc gtattagctg catggttggc
    22081 atgtcggctt tgtcgtacac tttgttaaac agtctggagg aaataatgaa agaaaccgat
    22141 attgcaggta ttttaacgtc cactcacacc attgcgctgg tgggggcaag cgacaaaccc
    22201 gatcgtccca gctatcgcgt gatgaaatat ctactcgacc aggggtatca cgtcattccg
    22261 gtttcgccaa aagttgccgg caaaacgctg ctgggccagc aggggtatgg cacgctggca
    22321 gacgttccgg aaaaagtcga tatggtcgat gttttccgta attcagaagc ggcgtggggc
    22381 gtggcgcagg aagctatcgc cattggcgcg aaaaccctct ggatgcaact gggggtgatt
    22441 aatgagcaag cggcagtact ggcacgggat gccgggttaa atgtggtgat ggatcgctgc
    22501 ccggctattg agatccctcg cctgggcctg gccaaataaa aaatccccgg aaggcaaaaa
    22561 ccttccgggg atttgttcag ggattagtta cgcagacgcg gggcctggag ttgtttgcgg
    22621 atggtctgcg ccagctcatc catggatggc tgttcaggat gttcatcctg caattcgcta
    22681 cttagctggg cttcagccag gtaggtatga accggtaggc cgttatcgtc ctccatcacc
    22741 acgtgatacc acggagcggc gcgaagctcg tcattcaccg ccagttcatc aggcgatggt
    22801 tcagagagcg aataaaccgg gtcgatatcc acgaccactc cgagataacc taacagggaa
    22861 tggcggacct gctggccgat accgaatttg ctggcaatca tagtcacctc ccgggaaatc
    22921 tttacccttg atataagggt aaaattccac atttcaagtt acatgacgcg acaggcaaac
    22981 cctttcagat atagcccttc cgggtaggta gcgatcaccg gatgatcggc tgcctgacgg
    23041 aactgctcta taaattgtac atcacggccg gcatcaattg cggcatccgc gatgattttc
    23101 tgaaataaat cgctggtcat cagaccggaa caggagaaag tcaggagaat accgtcttca
    23161 ttcagcaact gaatcgccag catgttgata tctttatagc cacgacacgc gcccatcaac
    23221 tggcttttat tctcaacaaa cttcggcggg tccatcacga taacgtcaaa tttttcaccg
    23281 cgatcgcgat aagtacgcag caatttaaag acatcatcac ggacaaactc agccttgctc
    23341 agatccagtt tgttcagctc aacgttctgc cgtgcaatat ccagcgcttc ctgggaggta
    23401 tcaacgctga caacctggct gcaaccgccc atcagtgccg atacggcgaa accaccggta
    23461 taggagaaac agttcagcac acgtttattt tcaacgtagc ggcgggtagc caggcggctg
    23521 tctcgctggt ccaggtagta gcccgttttg tgtccgtgct gaatatccac cagcagcttc
    23581 atgccgtgtt cttcaatcgg cagcagggca ggcggcaact cgccggtgac ggggccttgg
    23641 gtcagctcca tcccttcttt tttacgtacc gcaacatcgc tgcgatcgta aatcgcacat
    23701 tccgggtaca gcgtttgcag ggcactaatt aatgccgcgc gctgatactc tgccccagca
    23761 ctcagcagtt gcagcaccag aaaattaccg aaacgatcga tagtaatacc cggcaggcca
    23821 tcagattctc cggcgattaa acgatagctg tcgaggccat ctttttgcgc cagccagtca
    23881 cgccattttt gtgcttgttg caaacggcgg gaaaaaaaag caatgtcgat agactcagac
    23941 gggtcaaacg tccagacgcg cgcccggatt tgcgaagctg gcgaataagc gccgcgtgct
    24001 aaccattttc cctgatgatc aacaatatcg atggtttcac cgaggctggc tttaccttcc
    24061 atgcgggcaa cggccccgga aaagacccac ggatggcgac gaagtaatga tttttcgcgc
    24121 cctttggcta acactaaacg tacactcata atttactttt ctgtcgatgc aaagaaatgg
    24181 gcgtcattgt ccggagtttt ccatggattt gcaacgcgtc cattaaggat aaggagagca
    24241 gcaatgtcga aagtctgcat aattgcctgg gtttacgggc gagttcaggg cgtaggattt
    24301 cgctacacca cacagtacga agcgaaaaga ctgggtttaa ccgggtacgc caaaaatctt
    24361 gacgatggca gcgtggaagt ggttgcttgc ggtgaagaag ggcaggtaga aaaattagtg
    24421 cagtggttaa agagtggcgg cccgcgttct gcgcgtgtag aacgggtgct cagcgagccg
    24481 catcatccct cgggggaatt aaccgatttt cgtatccgtt aaatacattt taccggtttt
    24541 ggcaggccag caattttggt ggcttgctta gccggacctt tcggaaacag tcggtataaa
    24601 tagcggctgt tacctttttc ttcgccaaat ttattcgcca tcgcttttac cagcatacga
    24661 atcgccggag aagtattgaa ttccagatag aaatcacgca caaaacgcac cacttcccag
    24721 tgttctggcg acagcgaaat cccttcgttc tttgcaatca ccactgccag cggttcactc
    24781 cactggctgc tttctttgag atagccttcg gtatccgttt ctatctcttt accttcgaag
    24841 atcagcataa ttattactac cttaatcaga ctggcggcag tgtaacaaaa aacaaagccc
    24901 cgcataaagc gaggctatga aagtgttagc gggtgagatt aatcgcggct ggcgaagccc
    24961 agaatgctca gcaggctgac gaagatgttg tacagcgaaa catacaggct aaccgtggca
    25021 cgaatatagt tcgtttcacc gccatgaatg atgttgctgg tttcaaacaa aatagcgcca
    25081 gaggagatca gaatgaagac cgcgctgatc gccagatgca gagcaggcag ttgcaggaag
    25141 atattagcaa ccataccaat cagtaccacc acaatacccg ccatcagcat accgccgagg
    25201 aacgacatat ctttgcgggt ggtcagcaca tatgcagagc agcagaagaa cactaacgcc
    25261 gttccgccca gtgccatagc gattacgtca cccattccgg cagacagata ggtgttcaga
    25321 attggtccga ggatataacc cagaaaaccg gtaaaggcga atgcggagat aatcccggtc
    25381 ggcttattcg ccgttttata ggtcaggaac atcaaaccat acatacccac cagcgtcaga
    25441 atcagacccg gagatggcag catcagcacc gtactggcag ttgcggtaat cgccgaaaag
    25501 gccagcgtca ggctcagcag aaaataggta ttacgcagca ccttatgggt gctaagcagt
    25561 gatgtacggt catgtgaaga actaacaata cgatccattg agtcactctc tatgacagat
    25621 gtaattaatt aagcagcata atgataatgc gtaagggcac ccagaagttt tacccatctt
    25681 tacgcatttg atctggaaca ggtttaacag cggattatca ggtcattaag caaatataac
    25741 gccctgagaa tttcgacagg caaaagaaaa aggggttagc atttagctaa ccccttatct
    25801 tatctggcgg aagcgcagag attcgaactc tggaaccctt tcgggtcgcc ggttttcaag
    25861 accgaagaaa aacaaataaa aatcagtgta atacgtgaaa ttgctgaaaa gttaatcaaa
    25921 tttaacacca agaaaaatca aggtattagg aagttgctca tagctagtat accaagtctt
    25981 tttgccagaa atttttctgg attgtctact tgctaaccac gagcggcaaa tccagtgttg
    26041 gtgtgatttt tgttttacga tcgtaaatca acacctgatt ttctgtttta tgcccgctga
    26101 aaatttgttt gtcgcgactg ctgccttcgt aatctgagat ccccttggct tttatgtcgt
    26161 gaaaattgca cccgaacgga acgccaactt tttgctcggc tgcgcgttta gcctgattcc
    26221 accagttatt cagcgtttta gctatgactt tcccgccttt ggttgtattg atcacatatt
    26281 cgcatgtaca ggaagataca tttcgggcta actggatcgc tgtacgtaat cgcggtgacc
    26341 attccttgat ttgtttggtg ccggttttgt tttgctcaat gtagatccct ttatccataa
    26401 tatcctgcca tttcaactca agcacatcac cgagtcttgc cgcacagaga taggaaatct
    26461 ccattgcaat gcgtaactgt ggaattgctt ccgcatatat cgccgcatac tgttcatcgg
    26521 tgatgtaaac agtgcgggct ttaagagaga attttctgac tcctttgcat gggttattct
    26581 tcacataccc acgctcatat ccccacccgt atactcgact caggcttgcc agttcatgat
    26641 ttgcctgggt cttgctctca agccctcgtt tatccatgaa aattcttacc tgctcagttt
    26701 tgacattatc agcaagcact tttccgaata ccatcagcaa cgccttctga tgttgtcgat
    26761 aatctttttg ggttcggggg gccagttctg taaatgcagg ggagtccata aacatgtgcc
    26821 acaatttagc tacggtcatt atgttgtgga gttttgcttt ttccagttca taattttgcc
    26881 agactttagc tacgctggtt ttccgcactc ttcctagccc tatacttctt gtacttcctt
    26941 cgggtttcca cacgtaactg taaccattcg atctaacccg cggtggcagt acattatctt
    27001 ttttattttt tcttggtctt cccattgttc agcgcctcaa aatcgggttc agcagaaacc
    27061 agttcagatg tttttggcat cgttgtcaga ccgtgtggaa tatccctgcg gagaactatg
    27121 ggttcgtttt tagggccgat tacaaatggg atgccgtgca gtcttaactg gtgttgctgt
    27181 tttgtgtatc gctcgtattt cgtgatctct ttaatctctg ctggagatag agttaattcg
    27241 tacatgtggt cacgtgcctt tacagcatga ccgccgccac tatagctggt gggcggcgat
    27301 atgggttgaa catcaaaaat cagcctgact cgggagcagt ttttgccaga tggctgaaac
    27361 gtatttcgcc tggtaacgtg catcgttcag agcgttatgg cgttcacctt cgaatggaat
    27421 agtcgttctt gcgtcgaaat ccatcaccag tcccagagca accatcgttc ttacatcgcg
    27481 atcattggtg taacgccatg ggcaggggat cccctgccgt tcatatgaac ggcgtaaaat
    27541 cacgttgtcg aaagttgcac cgttacccca gacctgaaca aaaaattcac cggagttttc
    27601 gtcgataaat tcccgtaatt gcagcagtgc atcatctaac gggatttcat cggtaagaat
    27661 ggcggattgc gcttcgcgtg actgtttcag ccaccactta atggtgtccc gatcaatgac
    27721 tccgccagca gtttccagat cgatagtttt gctgaattct ggccccatct ctccggtttg
    27781 cggatcgaaa aatattgcac ctattgagat aatcggggca tcaggatttt ttcccattgt
    27841 ttcaaggtca atcatcagat ggtgccacaa cctgctggtg gatgagattt catgatgacc
    27901 gttcacttta attaagggat ttgctgtttc gccagtttta ttatcgctgg cgtgatactg
    27961 atcgctgcca gtgttctcct tgtgcagatg ttcagtgcct tccatttcct ccggatcatt
    28021 tttctgagct tcagcctgat tctcttcatc gaatgtttcc aggtaggttg cgttccccat
    28081 caccgcacca cagtcaggac agttgccgcc accggtctga ccgcatgcgg tgcaaacttt
    28141 ttccagttcc tgttgcactg ctggttctgg ttgttgttct tctggcccgt tttgttgcgt
    28201 atccgggctg ttttgttccg cttctggctc attttgtttc acgttgggct gattctggtc
    28261 ctcagcgtcg cgactctgga tccctttcac ccatttcgga tcattcgggt cgctaatccc
    28321 ttcaacaaat tcaccacgtg atgcagcaag caatttatcg gcgtcaggct ggctgatatt
    28381 ggctgcctgc ataattttgt ttacttcgtc agcggtaact tttaccggct ctgattgttc
    28441 ggaatcttca gcggtatcca cattttgcgg taagcccgtg tatgtgccat tttttcgggc
    28501 aaaatattct tcttttgaga tttcggtacc gccggcagcc agcgccttgt ccagaccaga
    28561 aagtttgttt gccctgccgt atttttcccc gcccttatcg gtaaagacga aataaaatgg
    28621 tccttcacgc tctacagatg gttcagcttc caccaggatt tcattttttt gagtatcgga
    28681 ttctgccgtc tccactggag cagtttgtgc tgctgacggc tggagagcat cagtagagct
    28741 ctggtctgtt tcttcatgtt caaacacgcc ctttgtcgtc aggtattcac tgatatattt
    28801 gttcagtgca acgggatctt tgtgaatatc gatcggacgt tcgcgaacaa ggccaaaaat
    28861 agtctgacgg tcatagccaa gtgcttcggg ctgtttgcgc attgatgctg agatacgctt
    28921 ccagtcttcg cgatccttgt cgataacctc atttgtcgcc cagcgatgga tggtaccgtc
    28981 aatgtttcct gcatccacat caccgggcca gagggcgcag gccagttcat tgtcgagtgt
    29041 tttccatgtc tgtttgtatt cgcggcgaat ggcaacagtg acagccccgg ttttttcagc
    29101 agcgttttca gtgttctgtt ggttgactct ggcgcgggtg agatcaacaa cagacgtgta
    29161 ttttccggtt tccttgcgtt caccttcgcg acgttttttc cagatgcgca tctctgcctg
    29221 aatttcaggc catttagcgc caggctcaca tttatgctta acccacccga tggcatgcag
    29281 tttaagttca ggatacatgg cgttaacttc tggcattttc atcaacgctt caacgatatg
    29341 tccgccgaat gttgccatgt cttcctgtag taattcctgt gcgctaatca ccatatcaac
    29401 ggtgatgttt tcgcatgtgt cgaatttaac caggactgcg ttctgtactt caggggacag
    29461 cttgtcaaaa ttgacgttca tcggatcgga ttctggttcg accggtacaa aggaagctga
    29521 ctcctcatcc cagcggtttt cctgcatata ttcggtatcc caggaatcga gggcagggcg
    29581 gggtatgccg ggtttatcct cgcagacaat aaatttataa gcgcagtcct gagctgccgg
    29641 gaattgctcc agaaattgcc agtgaaattt tgcgcgtgcg cgacgctcat caccggcttc
    29701 aatggcagtg gccaccgcaa cagcgctatc ttcttttatg gcctgttcgt caggaatagc
    29761 agcgcaaata aagattttac tcattgtgtt ttaacctcat tacagattta agggtgaaca
    29821 aatccctgcc attgctggca tataaaaatg aaaccggata ttaattacgg tgctgtttta
    29881 aagtcctgcc ggtatttcgt tattattagt gtgagtagtt ttatctaccg gataacagtt
    29941 accgggaatt ttttgttctg ctgctgcagc catgcattct ttcattgaac cgtataagcc
    30001 agtcaccagc tcaagagatt cgccggaaac aagataaact gtcagaacga gtgcaaatgt
    30061 tgtattcatt gtttatatcc tttttgcagc aggtccagac gagccagcat tgaaggaatg
    30121 catacttcat ttaacaggtc ctgctcgagt tttctctgct taatggcgtc ttcaataaat
    30181 gttttgtctc cagtgataac gccaatttcg aaacgaagtt cagacgtgct ggcattacat
    30241 gataactttt ccattatcgc gtcctcaaca atgaattttg tgatgcggtg cctggtgcct
    30301 ccaggtgacg ttaaccagtt aacaattaac gccggataca gagcattctc gttactcagc
    30361 aaatgaccgc tttaccgatt taactgttcc gcgtgcgctg agccgcattc accgcatcac
    30421 aaaattcact tttaaaaaag ggcggcagag cagtcacgga gtaaaactga taccgccaaa
    30481 tgtcaccaga atattgataa cagagggcgt tgcagcgggg ttgtcactta agcgtatggt
    30541 caacctgaca acccggtgtc ctcaacgggg aaagaataac cccgccatac ttaccgccgc
    30601 gccatttcgc ggattgccac aaccggaagc gcacggtcga attaaattta acgacggctc
    30661 taagggagaa ggacttcgcc gtgcactttc gtgttatgcc ctgacttttc agggatatat
    30721 ccttttagta aactgtcagt gccggattct tatctgtgtc cggcgcacga ccacacgtag
    30781 cagcgtgttg gtctccattt ttaattcagc ccttaatgga ggataaaatg ccaaacaaac
    30841 catgccctgc ctgtaacgct ctttctggct ttttagagaa gggcggttat tacattttta
    30901 actgtccaga acatgttgaa tttcatatat caaaacttga cagtatcatt acaaagccaa
    30961 atcagtatca gtctgattta ctgaataaag agttaaacgc cgcccgagat taagggcgta
    31021 gtggcccatt aaaaatattg caaagaccaa ctattgttga actggattaa accagtaatt
    31081 ctctattgct cttattgtta ccatcggatg attgccgggg gttattttaa gttcgggagg
    31141 aaaaggctct ccatcagcag ccatcattga gtatgtgaaa tccgccgacg cagtcccttt
    31201 catgataatt ccgaattttt caagtaatga gagcagttcc ggtaatcctg tgtcatctga
    31261 ataactttcc tcatagaaag aagcgattgc cgcaggagat gacagttctc gctctgcaat
    31321 ttttttgtag ttttccagaa ttcttttttt gaatactttt cctcgacatg aaaacccatc
    31381 accgaggcaa tccgctttgc agtttcttca gtattctggt tgtttgttac cgaaacagag
    31441 tacaatccgg taaacgcatc gcgcacatta cgagccatat tatcaatgta tttcttcgtt
    31501 gcagattcta attcaacttc gttcagacgt tggtgaagtg catgttctgc aatttcctgg
    31561 atttctttta gtaaatcttt aaattccatc tttaactccg ttagtcgtta gattttgccg
    31621 aactggaaag cgcctgttta aactcactga agctgagagc ttcctcgcct tcggcaaggc
    31681 cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc gaaatccata tcactcacct
    31741 gagtttcttt ccagccagcg acgggcacca ttttcggttt taaacgtttt gcttttggta
    31801 tacgtcatcg cggtgaatgt accgtcctgg ttgggaaaca cgccgtacac cagagattcg
    31861 ttgttgccaa gatcgatagt atccatgttg acctcatttc cccttaacgc cgggtagcgg
    31921 aacaaaaacc tgctgcatag ttattaaagt tgaaccctgc cgtcatgttc atacgcctcg
    31981 ggctggctac ttaccccctg accactgctt ggtaactcga agtattgccc ggcgttctgt
    32041 ggggcggggt gggtggttgg tgtatgtaat ctacaattaa aaactgtttt gtgtcaacag
    32101 tttttaattg ttgttttggg caaaaaaatc cctcgaaaga gggagtatga aaattgttca
    32161 actcagatag agaagggaaa ttgtcgccga gaatgtgtca cgcttacaat ctcaatgctt
    32221 gaagcagcta ctctgtacag gattatgtag ttagggtggg tcacgatctc tctcaatcca
    32281 gaaacccgtt cgcttggtgg atataagtac ggatgctcag atagaggtaa taccgatgtt
    32341 tcaatgcgta tttttagtct acgtgctgcc ggtgggttct ccttggcgat gtaagttatg
    32401 atctggcgca aatcatcgcg agcagacggt agccataaaa tgggtaacat tactcgctct
    32461 tgttagttac agcaatttga gcaataagat tttccatttc agccattacc tcgtcatgtg
    32521 gaattgcggg gcgagtgtct gcaaggcttg acgttacttt agtgcgcaac cattcgttgt
    32581 aactgttttc ttgttcggta gtttcgaatt ctgaaactat cggagaaagg gctgtaccca
    32641 tggcataact cctcttcttg tactgtggtc acgcccggcg gcttttttgt gccgccagcc
    32701 acctagcaat ggtttcttcc attgattttt tcttgtcttt gatttcttga agcatttttt
    32761 cttggtcttc ctcaggaaac gcactaaaag cttggagcag ttcgcgttgg cgaggaccaa
    32821 ttttcatcgt gtcaggggtg aaaatttgct cacactcttc aggaggcaat aaaaaccaat
    32881 gcaatggatg ccctgaaacc tcaaccagtt tatccaaact tgaggcttta ggtgtagcct
    32941 taccgctgac ccattgttga acagtttgtt gtgtcacacc aattctacgg gcaagctcag
    33001 cctggctcca tccagtttcc tgaagaagct tgctgattct gtacatagat acttctaggg
    33061 cgctcatcat tattcaattt tacaggtaaa tactgttaaa agcatcacaa taaaaaactg
    33121 ttgattgcat acagtttttt attgtaggct ttgcttatag tttttagagg agggcaaaat
    33181 gctagatagc actcgcgaaa aaattaggca gaaatacact caggctgaaa taggtcgtta
    33241 tatgggggtc gctcaacaga ctgtttggca atggtttagc tttggcgttc ccccaaagca
    33301 ggtaattccg ttatgccaac taatgaagtg ggaagttacc ccgcatgaaa tccgcccaga
    33361 tatttatcct aacccaaccg acggtttacc tgttggatgt aaggttaaca catcaaatgc
    33421 gccggagttg attcatgaaa atcaagcatg aacacatccg catggcgatg aatgcctggg
    33481 cgcatccgga cggcgaaaaa gtaccggctg cgaaaattac caaagcgtat ttcgagctgg
    33541 gaatgacgtt cccggaactg tatgacgaca gccatccgga agccatggct cgcaatactc
    33601 agaaaatttt ccgctgggtg gagaaagaca ctcctgatgc ggttaaaaaa attcaggcgt
    33661 tgttaccagc gatcgaaaaa gcgatgccgc ctccgctggt ggcccgaatg cgcagccaca
    33721 gttccgctta ttttcgggag ttggtagaga cgaaggaacg gctggtgaaa gatattgatg
    33781 atttcgttgc atcagcgatc gttctgttcg atcagatgaa tcgtggtggc ccggcaggaa
    33841 acactctggc tgtgcattaa ttgggtaata aatatgagta atgacaaaaa attgacactg
    33901 agcgtttacg aaaacagtcc gcacatctgg cgtggcggtt tatctgatgt ggagctggca
    33961 gagtggttga tacataaagc taatgcgctg ctctggcgtt tgtcagccag agaacagcgc
    34021 aaggaaacca gaataaagct ggctgatgca gaagcgtgtg ccgggcttat tgaggattat
    34081 acaaatcttg gtatttcttc agcagagagt gatcccattc agcctctgag cagggagtca
    34141 atccagcacg ctggttgtat ggcacatctt gtaactgctc gtcaacatga ggtgggtatt
    34201 ggatcacttc cggtgggata ttcgctgatt ccagagctgg ttgaagcaag aaaatcagtt
    34261 cagaaaaaga gagatgacgc acttcaatta ttgagagagc actatggcgc gataccagaa
    34321 tgcgaacagc atcgataccc tgaaggttat gaatggatgc agtctctttt tgaagttcgc
    34381 taatcaatat gtcgagacga aggtatgttt cggcgcgcag ccaggctctg taatccggga
    34441 gcatttcggg gctgttacac cagcggtttg ttgctgcaac atttaataca tgagcctgat
    34501 aaaggctttt caaaaaatac atgtcgaacc tcctctggtt ctgtcgattg ggaaccacag
    34561 attatatccg gaggaaggtt cggcaccaga tgaggtagcc atgcgtgatt acgcaaaagt
    34621 ttctccgcga ttctggctgg gagaaacggg gagagaactt agaaaggcgg gtgcagaagc
    34681 gcaagttgtt gctttttacc tgatgacatc ccctcacgca aatatgctgg gtttgtatta
    34741 cctgccagtt ttataccttg ctcatgaaac cgggcttggt ctggaagggg cttcaaaggg
    34801 gcttaaaagg gctgttgaag ctggtttttg tagctatgac catgatgcag agatggtctg
    34861 ggtccatgaa atggcagcct ggcaggttgg ggaaacgttg aagcctggcg ataaccgttg
    34921 tgcaggtgtc aggaatgagt atgcatcatt acctgaaaac gcttttctgt cagtgtttta
    34981 cgacagatat aaaacggatt tccatctgga tgtgaggcgg aataatagcc gaaattcggt
    35041 aaggggcttc gaaggggctt ttaaggggct tcgaagccaa gaacaggaac aggagcagga
    35101 gaaagaacag gaacaggaca aaaacactat ggttcatggc aaaaaaaaca ccacgaacca
    35161 ggcaggggat gttcagaccg tcaatcctgg tcagccagca ggcacgacac cggaagccga
    35221 ttcagcgtat gcgctgaaag ccgattcggg cgctgtgcag caggtgatga ccgcagggtc
    35281 ggagcaatca caccaactgc agcagcctga agccgattcc gccattcagc gggaagccga
    35341 tcgggtagtc ccggaaagca ccgggcagtc tgtgggacga gtggattatc cggatgtgtt
    35401 cgaacaggtc tggcgggaat acccgttgcg tgctggggca aacccgaaga aatccgcttt
    35461 cagtgcctgg aaggccagat tgcgcgaggg ggtgccacca gagaccatgc tggatggtgt
    35521 gaggcgttac gcgagatacc tggcggc
//
LOCUS       DAZFXT010000042        34689 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.042, whole genome shotgun sequence.
ACCESSION   DAZFXT010000042 DAZFXT010000000
VERSION     DAZFXT010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.042"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(126..878)
                     /gene="tcyN"
                     /locus_tag="ACHKW0_002144"
     CDS             complement(126..878)
                     /gene="tcyN"
                     /locus_tag="ACHKW0_002144"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     TcyN"
                     /protein_id="HFO7474978.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(875..1543)
                     /gene="tcyL"
                     /locus_tag="ACHKW0_002145"
     CDS             complement(875..1543)
                     /gene="tcyL"
                     /locus_tag="ACHKW0_002145"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HFO7474979.1"
                     /translation="MQESIQLVIDSLPFLLKGAEYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1558..2544)
                     /gene="dcyD"
                     /locus_tag="ACHKW0_002146"
     CDS             complement(1558..2544)
                     /gene="dcyD"
                     /locus_tag="ACHKW0_002146"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019148 - D-cysteine desulfhydrase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HFO7474980.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2649..3449)
                     /gene="tcyJ"
                     /locus_tag="ACHKW0_002147"
     CDS             complement(2649..3449)
                     /gene="tcyJ"
                     /locus_tag="ACHKW0_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015276 - ligand-gated monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7474981.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3537..4088)
                     /gene="fliZ"
                     /locus_tag="ACHKW0_002148"
     CDS             complement(3537..4088)
                     /gene="fliZ"
                     /locus_tag="ACHKW0_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HFO7474982.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTAREYVVRLRRLGNHLHEQNISLDPLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4134..4853)
                     /gene="fliA"
                     /locus_tag="ACHKW0_002149"
     CDS             complement(4134..4853)
                     /gene="fliA"
                     /locus_tag="ACHKW0_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HFO7474983.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5018..6511)
                     /locus_tag="ACHKW0_002150"
     CDS             complement(5018..6511)
                     /locus_tag="ACHKW0_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin"
                     /protein_id="HFO7474984.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLTSIQSEIQQRLSEIDRVSGQTQFNGVKVLASDQDMTIQVGANDGETITIKL
                     QEINSDTLGLSGFGIKDPTKLKAATAETTYFGSTVKLADANTLDADITATVKGTTTPG
                     QRDGNIMSDANGKLYVKVAGSDKPAENGYYEVTVEDDPTSPDAGKLKLGALAGTQPQA
                     GNLKEVTTVKGKGAIDVQLGTDTATASITGAKLFKLEDANGKDTGSFALIGDDGKQYA
                     ANVDQKTGAVSVKTMSYTDADGVKHDNVKVELGGSDGKTEVVTATDGKTYSVSDLQGK
                     SLKTDSIAAISTQKTEDPLAAIDKALSQVDSLRSNLGAIQNRFDSAITNLGNTVNNLS
                     SARSRIEDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6766..8178
                     /gene="fliD"
                     /locus_tag="ACHKW0_002151"
     CDS             6766..8178
                     /gene="fliD"
                     /locus_tag="ACHKW0_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0009421 - bacterial-type flagellum
                     filament cap [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HFO7474985.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPTIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGNYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8193..8603
                     /gene="fliS"
                     /locus_tag="ACHKW0_002152"
     CDS             8193..8603
                     /gene="fliS"
                     /locus_tag="ACHKW0_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HFO7474986.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8603..8968
                     /gene="fliT"
                     /locus_tag="ACHKW0_002153"
     CDS             8603..8968
                     /gene="fliT"
                     /locus_tag="ACHKW0_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HFO7474987.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNFS"
     gene            9046..10533
                     /gene="amyA"
                     /locus_tag="ACHKW0_002154"
     CDS             9046..10533
                     /gene="amyA"
                     /locus_tag="ACHKW0_002154"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7474988.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSKFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTINLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10567..10980)
                     /gene="yedD"
                     /locus_tag="ACHKW0_002155"
     CDS             complement(10567..10980)
                     /gene="yedD"
                     /locus_tag="ACHKW0_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YedD"
                     /protein_id="HFO7474989.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11167..12372
                     /gene="yedE"
                     /locus_tag="ACHKW0_002156"
     CDS             11167..12372
                     /gene="yedE"
                     /locus_tag="ACHKW0_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HFO7474990.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12369..12602
                     /gene="yedF"
                     /locus_tag="ACHKW0_002157"
     CDS             12369..12602
                     /gene="yedF"
                     /locus_tag="ACHKW0_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HFO7474991.1"
                     /translation="MKNIIPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12711..13379
                     /gene="yedK"
                     /locus_tag="ACHKW0_002158"
     CDS             12711..13379
                     /gene="yedK"
                     /locus_tag="ACHKW0_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HFO7474992.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAREWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPV"
     gene            complement(13772..14086)
                     /gene="fliE"
                     /locus_tag="ACHKW0_002159"
     CDS             complement(13772..14086)
                     /gene="fliE"
                     /locus_tag="ACHKW0_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HFO7474993.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            14301..15959
                     /gene="fliF"
                     /locus_tag="ACHKW0_002160"
     CDS             14301..15959
                     /gene="fliF"
                     /locus_tag="ACHKW0_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009431 - bacterial-type flagellum basal
                     body, MS ring [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body MS-ring/collar protein
                     FliF"
                     /protein_id="HFO7474994.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMMQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            15952..16947
                     /gene="fliG"
                     /locus_tag="ACHKW0_002161"
     CDS             15952..16947
                     /gene="fliG"
                     /locus_tag="ACHKW0_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HFO7474995.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            16940..17626
                     /gene="fliH"
                     /locus_tag="ACHKW0_002162"
     CDS             16940..17626
                     /gene="fliH"
                     /locus_tag="ACHKW0_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HFO7474996.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            17626..18999
                     /gene="fliI"
                     /locus_tag="ACHKW0_002163"
     CDS             17626..18999
                     /gene="fliI"
                     /locus_tag="ACHKW0_002163"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HFO7474997.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            19018..19461
                     /gene="fliJ"
                     /locus_tag="ACHKW0_002164"
     CDS             19018..19461
                     /gene="fliJ"
                     /locus_tag="ACHKW0_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export protein FliJ"
                     /protein_id="HFO7474998.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            19458..20585
                     /gene="fliK"
                     /locus_tag="ACHKW0_002165"
     CDS             19458..20585
                     /gene="fliK"
                     /locus_tag="ACHKW0_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HFO7474999.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSIQGRVTGNSGVDIFA"
     gene            20690..21154
                     /gene="fliL"
                     /locus_tag="ACHKW0_002166"
     CDS             20690..21154
                     /gene="fliL"
                     /locus_tag="ACHKW0_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009425 - bacterial-type flagellum basal
                     body [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0071973 - bacterial-type
                     flagellum-dependent cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HFO7475000.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGVTLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            21159..22163
                     /gene="fliM"
                     /locus_tag="ACHKW0_002167"
     CDS             21159..22163
                     /gene="fliM"
                     /locus_tag="ACHKW0_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HFO7475001.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            22160..22573
                     /gene="fliN"
                     /locus_tag="ACHKW0_002168"
     CDS             22160..22573
                     /gene="fliN"
                     /locus_tag="ACHKW0_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HFO7475002.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            22576..22941
                     /gene="fliO"
                     /locus_tag="ACHKW0_002169"
     CDS             22576..22941
                     /gene="fliO"
                     /locus_tag="ACHKW0_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliO"
                     /protein_id="HFO7475003.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     VPKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQPDFQSVMKNLLKRSGRS"
     gene            22941..23678
                     /gene="fliP"
                     /locus_tag="ACHKW0_002170"
     CDS             22941..23678
                     /gene="fliP"
                     /locus_tag="ACHKW0_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HFO7475004.1"
                     /translation="MRRLLSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            23688..23957
                     /gene="fliQ"
                     /locus_tag="ACHKW0_002171"
     CDS             23688..23957
                     /gene="fliQ"
                     /locus_tag="ACHKW0_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HFO7475005.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            23966..24751
                     /gene="fliR"
                     /locus_tag="ACHKW0_002172"
     CDS             23966..24751
                     /gene="fliR"
                     /locus_tag="ACHKW0_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HFO7475006.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            25041..25664
                     /gene="rcsA"
                     /locus_tag="ACHKW0_002173"
     CDS             25041..25664
                     /gene="rcsA"
                     /locus_tag="ACHKW0_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HFO7475007.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(25708..25896)
                     /gene="dsrB"
                     /locus_tag="ACHKW0_002174"
     CDS             complement(25708..25896)
                     /gene="dsrB"
                     /locus_tag="ACHKW0_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HFO7475008.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            26059..26286
                     /gene="yodD"
                     /locus_tag="ACHKW0_002175"
     CDS             26059..26286
                     /gene="yodD"
                     /locus_tag="ACHKW0_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HFO7475009.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            26584..27399
                     /gene="yedP"
                     /locus_tag="ACHKW0_002176"
     CDS             26584..27399
                     /gene="yedP"
                     /locus_tag="ACHKW0_002176"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050531 - mannosyl-3-phosphoglycerate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0051479 - mannosylglycerate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HFO7475010.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(27396..29090)
                     /gene="dgcQ"
                     /locus_tag="ACHKW0_002177"
     CDS             complement(27396..29090)
                     /gene="dgcQ"
                     /locus_tag="ACHKW0_002177"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator diguanylate
                     cyclase DgcQ"
                     /protein_id="HFO7475011.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPFNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(29261..29443)
                     /gene="yodC"
                     /locus_tag="ACHKW0_002178"
     CDS             complement(29261..29443)
                     /gene="yodC"
                     /locus_tag="ACHKW0_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HFO7475012.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(29522..30439)
                     /gene="yedI"
                     /locus_tag="ACHKW0_002179"
     CDS             complement(29522..30439)
                     /gene="yedI"
                     /locus_tag="ACHKW0_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HFO7475013.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            30612..31532
                     /gene="yedA"
                     /locus_tag="ACHKW0_002180"
     CDS             30612..31532
                     /gene="yedA"
                     /locus_tag="ACHKW0_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HFO7475014.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLMAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(31521..31991)
                     /gene="vsr"
                     /locus_tag="ACHKW0_002181"
     CDS             complement(31521..31991)
                     /gene="vsr"
                     /locus_tag="ACHKW0_002181"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HFO7475015.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(31972..33390)
                     /gene="dcm"
                     /locus_tag="ACHKW0_002182"
     CDS             complement(31972..33390)
                     /gene="dcm"
                     /locus_tag="ACHKW0_002182"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HFO7475016.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDSRRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDEADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMAKGEKDFDDP
                     QNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(33457..34152)
                     /gene="yedJ"
                     /locus_tag="ACHKW0_002183"
     CDS             complement(33457..34152)
                     /gene="yedJ"
                     /locus_tag="ACHKW0_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HFO7475017.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQGAAHDVCHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(34192..34473)
                     /gene="drpB"
                     /locus_tag="ACHKW0_002184"
     CDS             complement(34192..34473)
                     /gene="drpB"
                     /locus_tag="ACHKW0_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DrpB"
                     /protein_id="HFO7475018.1"
                     /translation="MEERLSRSPGGKLALWAFYTWCGYFVWAMARYIWVMSRIPDAPV
                     SGFESDLGSTAGKWLGALVGFLFMALVGALLGSIAWYTRPRPARSRRYE"
BASE COUNT         8487 a         9023 c         8783 g         8396 t
ORIGIN      
        1 acttttgcaa gccatcgcat tctcttatgt tattaatgag ttatgctgat ttgttaagca
       61 gttttatcac gcttgaaatg gcgtcccgcc ccgacaggtg aatcgtcggg gctgattttt
      121 tcttattatt gcagcagaaa cttctcgagg aactggcggg tgcgaggctg ctggggatcg
      181 gcaaataacg cttttgctgc cccctgctcg actatccgcc cctggtccat aaagatcgcc
      241 cggtccgcaa catcccgggc aaagctcatt tcgtgtgtca caatcaccat cgtgcgcttt
      301 tcctgcgcca gctggcggat ggtgttcagg acttcgccca ccagctctgg atccagcgcc
      361 gacgttggct cgtcaaacaa aatcacctca ggacgcattg ccagtgcacg cgcaatcgca
      421 acacgctgct gttgaccgcc agacaaacga cgtggatagc tggtttcttt accggccagc
      481 ccaactttcg ccagcagctc gcgagcgcgc gccgtggcct cttctttcgg ttcgcctttg
      541 acgatcaccg gcccttcaat aatgttttcc agcaccgtac gatgcggaaa caaattaaag
      601 ttctggaaga caaacccgac gtgctgacgc aactggcgaa tcagcgattt ttgctgactt
      661 aatgaacgtg cagtatcaat agtgatatcg ccaacggtga tcgttcccgc ttccggttgt
      721 tccagcagat ttatgctgcg tagcaacgtg gttttgccgg aaccactcgg accgataatt
      781 gccaccactt cgccaggctt tacctcgagg tcgataccgt gcagcaccgt ctgaccgtga
      841 aattttttca ccaggttctt aacttcaatg gcactcattt tggttctctc tcctggcgat
      901 taagttgatt ctcaaaatgg ttctgcaacg tcgataatac tgtcgccatg atccagtaga
      961 tgagcgaagc cgccaggtac atggtgaaca cctccagcgt gcgcgaggta atcaactgcg
     1021 cctgacggaa cagctccggc acctggattg tcgcggccag cgacgtatct tttaccagac
     1081 tgataaagct gttcgacagc ggcggcaacg ccacccgcgc cgcctgtggc aaaatcgcgc
     1141 gacgcatggt ctgccacggc gtcataccaa tactggcagc ggcttcccac tgacctttat
     1201 caatagaaga aatggcggcg cgcagcgttt cggcggcata ggcggcagtg ttcagcgaca
     1261 gaccaatcat tgctgacgga atgggatcta attcgatacc aaactgcggc aggccgtaat
     1321 agatcataaa cagttgtgcg atgagcggcg taccacgaaa aatagagata taaaaacgcg
     1381 ccagccagcg cacgggccag attggcgaca ggcgcatcag cgcgagaata aacccgagta
     1441 gtaagccaaa aaacatgccg ccgatactga gttgcagggt atactcagcc cctttcaaca
     1501 ggaacggcaa ggaatcaata accagttgta tactttcttg catgagcgtt cccggagcta
     1561 aacgtgggga tgataggcga acagcgcagg cgcgccgccg gtatgaataa acagaatcgg
     1621 cccttcatct ttgaagcgtt tctgactgat accgtcaatc agccccgcca tcgcttttcc
     1681 ggtatacaca ggatcaagca gaatgccttc cagacgcgcc agcaatttca ctgcttccat
     1741 gccttcgtcg ttcggcacgc cgtagccagg tgcaaaatag tcatcccaga gtaaaatttc
     1801 cgctgatgcg gtcagctcca gttctttcgc aatcgcctgt tgtaggttaa ccactttcgg
     1861 caattgatcg gcaacggaac gcgacacggt cacgccaatc agttcgcttt caggcatcag
     1921 gtgttccagc ccaacagcca atccggcgtg agttccggca ctgcccgatg cgaccaccac
     1981 cgacgaaata ttaaccgccc cttcacactg ttgcgcgatt tccagcgcac tctccacata
     2041 acccagcgcg cccagagcat tagaaccgcc aaccggaatg acatacgggc gaaagccttg
     2101 tgcttcgact cgcgtcgcca gctcttccag ttgggcattg ggatcggtca gtgcgtcgca
     2161 catttcaatc tgggtattga acagatccag caacaaacga ttgccgttgg ttaaatagtt
     2221 ttctgcggtt gtgccaatag gattttccag cagcgccacg cagtgcagac cgagtttcgc
     2281 cgcgactgcg gcagtctggc gcacatggtt agactggatc gcgccggcag taatcagcgt
     2341 atcggcacct tcgcgcaggg catctgccgc gagaaattcc agcttacgta atttattgcc
     2401 gcccattgcc atgggtgtga cgtcatcccg tttgatgaaa atttcccgtc caagataatc
     2461 agaaaagcgc ggcagatatt cgagcggcgt tggcgcgccg ataaactcca accgtggaaa
     2521 acgggttaaa ttatgcagtg gcataacagc ctccgatgtg tgttgttgtg attttcttat
     2581 tatgcacgct gaaaacgcgt aaataaaaaa ggcgctagtg aaagcgccct tttttgtcat
     2641 tatgctgatt atttggtcac atcagcacca aaccattttt cggaaagagc ttgcagagtg
     2701 ccgtcttttt gcatttccgc aattgcatca ttcaccgctt tcagcaggtc ttcatttcct
     2761 ttacgcagcg ccacgccaga ctcctgacgg gagaatgctt caccggttac tgccagcgta
     2821 tcgttggttt tcttcaccag atccagcgcc gccagacgat caacgaggat cgcatcgata
     2881 cggcctacgc gcagatcctg atatttggtc gggtcatcat cataggtacg cacatcgacg
     2941 ccctgaacat tctgccgcag ccactcttca tagttggtgc ccagaccgac acccactttt
     3001 ttgcctttca gatcagcagc cgttttaatg gtgccttcgt tacctttttt caccagcgcc
     3061 tgaataccgg aaatggtgta aggggtagag aaatcgtatt ttttcttgcg ctcgtcagaa
     3121 atggtgacct gattaatcac cacatcaatg cgtttagagt ccagcgacgc cagcataccg
     3181 tcccatttgg tcggttttag tgacgcctca acgccaagat gttttgccag ctgttgggca
     3241 aattccactt caaaaccggt taatttgccg tcatctccct gaaaactgaa cggcggataa
     3301 gtgccttcca gccctaccag cagcgtgccg cgctctttaa ctttattaag cagaccttca
     3361 tctgcaaaac ttttaacgct catgcccgca accagcgcta cggccatcac acccatcaat
     3421 gcctgacgtc ccagatgtgc taatttcata tttaccccga atgttgttat gtctgtttgc
     3481 agtgtagagc cattgtttgt aaacacaaaa acaactccgc tacatcttat tcttatttaa
     3541 tatatatcag aagcaggcag gctggaggat ttctgcacaa gtccggtaca cgtttgacgc
     3601 tgatagtgtt gatacttccg taatgcgatg cggtaattgt tggtgctggt ggtgggcagc
     3661 cacggggcga ggatttcatc aaggaaaccg tcctgcagcg gatcgaggga aatattttgc
     3721 tcgtgtagat gattccccaa acggcgcaga cgaacgacat attcacgcgc cgtaccaggg
     3781 ctcatttcag tttgctcaaa aagaaattgc ttaaagccga taatatcgaa aaaatcactc
     3841 tgcgttttgc aatgtaaatc accgcagaat cggcacaatg ccgcccatga tttttgttcc
     3901 acttgccagc cattttcatc aagcagcatg tccaggcggg aaatctcgat tttattcact
     3961 attttgccgt cgcgaacgag ggtaatgcgg tcgagtaatt tacggcaatg cgcgcaatgg
     4021 gtctggctgt gtttaaagtc tttaagatag cggcttaatg gccgtctttt caggtgctgc
     4081 accatcatta agaactcctg gtagtcaaaa ttaaagtgcg gcatttactg acgttataac
     4141 ttacccagtt tagtgcgtaa ccgtttaatg gcctggctgt gtaactgact gacccgcgat
     4201 tccccgacct ccagcaccgc gccaatctct ttgagattca gctcttcctg gtaatagagg
     4261 gttaatacca gtttttcgcg ctccggcaac gtttcgatgg cttccatcac ccgctggcgc
     4321 agattactgt ccagcagttg ttgtagcggg ttttctcgct gatgatcatc agtaaccagt
     4381 tcgatgctat cgccgtgctc ttcgcgccac tcatcgtagg agaagagctg gctgttattg
     4441 gtgtcgagca acatttggcg ataatcggca atatcgatcc ctaaacgttc cgctacctca
     4501 gtttccgtgg cgttgcggcc aagttcctgc tccagttgcc ctattgcctg tgccacttca
     4561 cgcgcgttgc gtcgcacgct gcgcggcacc cagtcacggc tgcgaagttc atccagcata
     4621 gcgccacgga tacgctgcac tgcgtaagtt gtaaatgccg ttccttgtag ggcgtcatag
     4681 cgttcgacgg cattaagtaa cccaatgccg cccgcctgta gcagatcgtc aagttccacg
     4741 ctcgcgggca gtcgaacctg caggcgcaat gcttcgtgac gcaccagcgg gacataacgc
     4801 tgccacagcg agtgtttatc cattacacct tcagcggtat agagtgaatt cacgataaac
     4861 agccctgcgt tatatgagtt atcggcatga ttatccgttt ctgcagggtt tttaatcgga
     4921 cgattagtgg gtgaaatgag gggttatttg ggggttaccg gtaaattcca tgcagaaaaa
     4981 accccgccgg tggcggggtt tgagcgataa gtgtaaatta acgcagcaga gacagtacgt
     5041 tctgcgtggt ctggttagcc tgtgccagaa cagaagtacc tgcttgttgc aggatctgcg
     5101 cacgagacat gttagacact tcggtcgcgt agtcagcatc ttcgatacgg ctacgggcag
     5161 aagacaggtt gtttacggtg ttgccaaggt tggtgatggc agagtcgaaa cgattttgaa
     5221 ttgcacctag gttagaacgc aacgagtcaa cctgagacag tgctttatcg atagcagcca
     5281 aaggatcttc tgttttctgc gtagaaattg ctgcaataga atcagttttc aggctcttac
     5341 cttgtaaatc actaacactg taagttttgc catcggttgc agttacaact tcggttttgc
     5401 catcgcttcc acccagttca actttaacat tgtcgtgttt gacaccgtca gcatcagtgt
     5461 aagacattgt tttaacggaa actgctcctg ttttctgatc aacattcgct gcatactgtt
     5521 taccgtcatc accaatcaac gcaaatgaac cagtatcttt gccattggcg tcttctaact
     5581 taaagagttt tgcacctgtg atagaagcgg ttgcggtatc agtacccaac tgaacatcaa
     5641 tagccccctt ccctttcacc gttgtgactt cctttaaatt accagcttga ggctgggtac
     5701 ccgctagagc ccccagcttc agcttacctg catcaggaga tgtcggatca tcctccacag
     5761 taacttcata ataaccattt tcagcgggtt tatctgaacc ggcaacttta acgtacaact
     5821 taccgttagc atcagacata atattaccgt cacgttggcc cggagtcgta gtgcctttaa
     5881 ctgtagctgt aatatctgca tcaagtgtat tagcgtcagc aagcttaact gtcgatccaa
     5941 aataggttgt ttcagccgtt gcggctttta atttagtagg atctttaata ccaaaaccag
     6001 ataatcccag tgtgtcggaa ttaatttcct gcagtttaat agtaattgtt tcgccgtcgt
     6061 ttgcaccaac ctgaatagtc atatcctgat cagaagccag cactttaacg ccgttaaact
     6121 gagtctgacc agaaacacgg tcaatttcac tcagacgctg ctggatttcg gactggatgg
     6181 aggtcaggtc actgtcagag ttagtaccgt tagttgcctg aacagaaagt tcacgaatac
     6241 gctgcaggtt gttgttaatt tcattcagcg caccttcagt ggtctgcgca acagaaatac
     6301 catcattcgc gttacgggaa gcctgagtca gacctttaat atttgctgta aaacggttag
     6361 caatcgcctg acctgctgcg tcatctttag cactgttaat acgcaggcca gaagagagac
     6421 gttcaatggc ggagctcaga gaagactgag atttgttcag gttattctgg gtcaacagcg
     6481 acaggctgtt tgtattaatg acttgtgcca tgatcttttc cttatcaatt acaacttgat
     6541 gttattgggc tgttgcccac ggtttctcac cgtacccctt gtatcggcac ctgaatttcg
     6601 aactttagaa aattttagtt tctctcctgt tttttcttaa cggctgaaat atccgtaata
     6661 tacaactcta ctctgcgcat catcttcgtg aatcaactat taaactttgc gcacaaattg
     6721 ccgataaccc gcttatctac tgtttgcaat caaaaggaat taggtatggc ttcatttaca
     6781 tctcttggcg ttggttcgaa tctgccgctg gatacattgc taaacaacct gaccatcgct
     6841 gaaaaaaaac gcttaaaccc aattactcag caacagagtg acaataccgc acgtctgaca
     6901 gcctatggca cattaaaaag tgcattagaa aaattccaga ctgcaaatac tgcgttaaat
     6961 aaagccgatc tgttcaaaag cactaccgtt accagtagca atgaagacct taaagtttcc
     7021 accgaagcag gtgctgctcc cggtatctat accatcagcg taacccaact ggcgcaggcg
     7081 caatctttac gaactgattc cccgacaatc attgcatcaa caaaagatgc actgggtgat
     7141 gagtcatcag atacacgcac aataaaaatt acccaagacg gtcgtaaaga gccgctggaa
     7201 atcaagctca ataaagatca gacgtcattg gatgagatca gcaaagccat taacgatgcc
     7261 gatagtggaa tttccgctag tatcgttaaa gtcaaagatg gtaactatca attggtattg
     7321 accgccagtg aaggtctggc caataaaatg accatatctg ttgagggtga cagtaaatta
     7381 aatgatctgc tggcttatga cagcaagacc aacaccggga atatgaagga actggttaac
     7441 gcgcaaaatg cacagctaaa cgttaacggt atcgatattg aacgttcaag taacaaaatt
     7501 accgatgctc ctcagggagt gactctcgat ttgaccaaaa aagtcactga cgtccgggtc
     7561 acggtgacaa aaagcaacga taaagcaacc gaagcaatta aaggctgggt ggattcatat
     7621 aactccctca ttgacacctt taacaccctg acaaaatata aagaagtaga cccaggtgct
     7681 gaagcacaag ataaaaataa tggtgcactt ctcggagaca gcgttgtacg tacaattcaa
     7741 agcggcatcc gcgcacaatt tgcaaacggt gccagcgacg gtgccttcaa aacattaaac
     7801 gagatcggga ttaagcagga cggcacgacg ggtaagctga aaattgatga tgataaactc
     7861 aaaaaagtcc tgaatgaaaa tacagcatcc gtacgtgaat tgctggtagg cgatggtaaa
     7921 gaaacaggaa ttaccaccaa aattgccacc gaggtaaaag gttaccttgc cgatgatggc
     7981 attattgata gcgcacagga cagtattaac gccacactga aaaaactgac caaacagtat
     8041 ctatccgtca gtgccagcat tgatgacacc gtggcgcgtt acactgcaca gtttacgcaa
     8101 ttagatacca tgatgagcaa actgaataac accagtacct atctcagcca acaatttact
     8161 gcaatgagta attcctgatc acggaggtca gcatgtatac cgcgagcggc tcaaaagcct
     8221 atgcacaaat tggcgtcgaa agcgccgtaa tgagcgccag ccagcagcag ctggtcacca
     8281 tgctatttga tggagtgctg agcgcactgg ttagagcgcg cctgtttatg caggacaaca
     8341 atcagcaagg caaaggcgtc tctttgtcaa aagcgatcaa catcattgag aacggacttc
     8401 gggtgagtct tgatgaagag agcaaagacg aattaaccca aaacttgatt gctctttata
     8461 gctatatggt caggcgcttg ctgcaagcca atttacgcaa cgatgtctcc gcagtcgaag
     8521 aagtggaagc attaatgcgt aatattgccg atgcctggaa agagtcgtta ctctcccctt
     8581 ctttgattca ggacccagtc tgatgaacca tgcaccgcat ttgtatttcg cctggcaaca
     8641 actcgtcgaa aaaagccagc tcatgttacg cctggcaacg gaagaacaat gggacgaact
     8701 catcgccagc gaaatggcgt atgtgaatgc ggtgcaggag atagcacacc tgactgaaga
     8761 ggttgccccg tccaccacga tgcaggagca gctccgcccg atgctgcgcc tgattctcga
     8821 caacgaaagc aaggtaaagc agttattaca gattcgtatg gatgaactgg cgaaactggt
     8881 cggtcagtca tcggtgcaaa aatcggtgtt aagtgcctat ggcgatcagg gcggctttgt
     8941 gctggctccg caagataact tctcgtgaat cttaacgagc taatggttcg cgaataatcc
     9001 gattacggct acgcttctaa tgttcccctt gaatggagtc gaagaatgcg taatcccacg
     9061 ctgttacaat gttttcactg gtattacccg gaaggcggta agctctggcc tgaactggcc
     9121 gagcgcgccg acggttttaa tgatattggt atcaatatgg tctggttgcc gcccgcctat
     9181 aaaggcgcat cgggcgggta ttcggtcggc tacgactcct atgatttatt tgatttaggc
     9241 gagtttgatc agaaaggcag catccctact aaatatggcg ataaagcaca actgctggcc
     9301 gccattgacg ccctgaaacg taatgacatt gcggtgctgt tggacgtggt agtcaaccac
     9361 aaaatgggcg cggatgaaaa agaagctatt cgcgtgcagc gtgtaaatgc tgatgaccgt
     9421 acgcaaattg acgaagaaat cattgagtgt gaaggctgga cgcgttacac cttccccgcg
     9481 cgtgccgggc aatactcgaa gtttatctgg gatttcaaat gttttagcgg tatcgaccat
     9541 atcgaaaacc ctgatgaaga tggcatattt aaaattgtta acgactacac cggcgaaggc
     9601 tggaacgatc aggttgatga tgaattaggt aatttcgatt atctgatggg cgagaatatc
     9661 gattttcgca atcatgccgt gacggaagag attaaatact gggcgcgctg ggtgatggaa
     9721 caaacgcaat gcgacggttt tcgtcttgat gcggtcaaac atattccggc gtggttttat
     9781 aaagagtgga tcgaacacgt acaggaagtt gcgccaaagc cgctgtttat tgtggcggag
     9841 tactggtcgc atgaagtcga taaactgcaa acgtatattg atcaggtgga aggcaaaacc
     9901 atgctgttcg atgcgccgct gcagatgaaa ttccatgaag catcgcgcat ggggcgtgac
     9961 tacgacatga cgcagatttt cacgggtaca ttagtggaag ccgatccttt ccacgccgtg
    10021 acgctcgttg ccaatcacga cacccaaccg ttgcaagccc tcgaagcgcc ggtcgaaccg
    10081 tggtttaaac cgctggcata tgccttaatt ttgttgcggg aaaatggcgt tccttcggta
    10141 ttctatccgg acctctacgg tgcgcattac gaagatgtcg gcggtgacgg gcaaacctat
    10201 ccgatagata tgccaataat cgaacagctt gatgagttaa ttctcgcccg tcagcgtttc
    10261 gcccacggtg tacagacgtt atttttcgac catccgaact gcattgcctt tagccgcagt
    10321 ggcactgacg aatatcccgg ctgcgtggtg gtcatgtcga acggggatga tggcgaaaaa
    10381 accattaatc tgggagagaa ttacggcaat aaaacctggc gtgattttct ggggaaccgg
    10441 caagagagcg tagtaaccga cgaaaacggt gaagcgacct tcttttgcaa cggcggcagc
    10501 gtcagcgtgt gggttatcga agaggtgatt taaattcatc cccggcggca agccggggag
    10561 atttcattac ggcagtggcg tcggtaacgg cgctttatcc agcgcggcgg cacactctgc
    10621 cgtcgggcga tccacacgct ccatcgtcat accatcgtac tcaatggtgt taccgtcacg
    10681 ctcaacctcg tacagctcac gttttacggt aacgttagtg agatcatcag acatcaatgt
    10741 caacttgccc ggcacggcaa tcacccgctg ccactgacgg caatccaggg tatcgccctc
    10801 tttagtgaca atcaggctgc ctatcgcttc cggactgacc agcgcacgtt gcggcccttt
    10861 ggtctgccag tagcccgcca gccagtccgg cgcgggggtt ttcacgacat tgttatagtt
    10921 ttcgacttcg gcacagcccg ccagcaacat gagcgcgcct gcaattgcga gttttttcat
    10981 cattcgtcct gcatgagaag aaaagaggat tgtggcatta aagccctgat gtcgccagcc
    11041 tttccaggtt cagagagaaa tttgatctaa ctattatttt tgtgaattat taccccgaaa
    11101 ataaatttgc atgatgtatg attcgcgatt cttgtttcaa accttctgag ttcagaggct
    11161 acattcatgt catggcagca attcaaacac gcctggttga ttaaattctg ggcacccatc
    11221 cctgcggtca tcgcggcggg tattctctcc acttactatt ttggcattac tggcaccttt
    11281 tgggctgtca cgggtgaatt tacccgttgg ggcggtcagc tcctgcagct gtttggcgtc
    11341 catgctgaag agtggggtta ttttaaaatt atccatctgg aaggatcgcc attaacccgc
    11401 atcgacggga tgatgatcct cggcatgttt ggcggctgct ttgccgcagc gctgtgggcc
    11461 aacaatgtca aactgcgcat gccacgcagc cgtatccgca ttatgcaggc cattattggc
    11521 ggtattatcg ctggttttgg cgcgcgtctg gcaatgggct gtaacctggc ggcgttcttt
    11581 accggtattc ctcagttctc gctgcatgcc tggttctttg ccatcgccac tgccattggt
    11641 tcatggtttg gcgcacgctt taccctgctg cccatcttcc gtattcccgt gaaaatgcag
    11701 aaagtttctg ccgcctcacc gctgacgcaa aaaccggatc aggcgcggcg tcgttttcgt
    11761 ctcgggatgc tggtcttttt cggtatgctg ggctgggcgc tgctcacggc gatgaaccaa
    11821 ccaaaactgg ggctggcaat gctgtttggc gtcggctttg gtttactgat tgaacgtgcg
    11881 caaatctgct ttacttcggc gttccgcgat atgtggatca ccggacgtac ccatatggcg
    11941 aaagcaatca ttatcggtat ggcggtgagt gccatcggga tcttcagtta cgtacagtta
    12001 ggcgttgaac ccaaaatcat gtgggcggga ccaaacgcgg taattggtgg tttactgttt
    12061 ggttttggca tcgtgctggc aggcggctgc gaaaccggtt ggatgtaccg cgcggtagaa
    12121 ggtcaggtgc actactggtg ggtcggtctg ggcaatgtga tcggctcaac cattctggcg
    12181 tattactggg atgatttcgc tccggcgctg gccaccgact gggacaaaat caacctgctg
    12241 aaaacctttg gcccgatggg tggcctgctg gtgacatatt tgctgttgtt tactgcgctg
    12301 atgttgatta tcggctggga aaaacgcttc ttccgccgtg cggcaccgca gactgctaag
    12361 gagatcgcat gaaaaatatc attcctgatt accgtcttga tatggtgggt gaaccctgcc
    12421 cttatccggc agtcgcaacc cttgaggcga tgccgcagtt aaaaaaaggt gagatcctgg
    12481 aagtggtgag cgactgtccg cagtcgatca ataatattcc actggatgct cgtaatcacg
    12541 gctatacggt gctggatatt cagcaagacg ggccgaccat tcgttattta attcagaagt
    12601 aatccctcat tcccgctgtg gccaacagcg ggaatatcgt tgcctctgta ccacatagat
    12661 tttacccgcc ccctctccta ttcttttcag tgtgctctca ggaggttgct atgtgtggac
    12721 gctttgccca atcccaaacg cgtgaagatt accttgcgct tctcgcggaa gatattgaac
    12781 gcgatattcc ctatgatccc gaacccattg gcagatacaa cgtcgcgccg ggaaccaaag
    12841 ttcttctgct cagtgaacgt gatgaacacc ttcatctgga tccggttttc tggggatatg
    12901 ctcccggatg gtgggataaa ccgccgctga ttaacgcccg cgtagaaact gcggccacca
    12961 gtcgtatgtt taaaccgctc tggcaacatg gtcgggcaat ctgttttgcc gatggctggt
    13021 ttgagtggaa aaaagaaggc gacaaaaaac agccttattt tatctatcgt gctgacggac
    13081 aacctgtttt tatggccgcg ataggtagca caccgtttga gcgcggtgac gaagccgaag
    13141 gatttttaat tgtcactgca gcagcagacc agggtctggt agatattcat gaccgccgcc
    13201 cgctggtact gtcgccagaa gctgcgcggg aatggatgcg gcaagagatt ggcggtaaag
    13261 aagcatcaga aattgctacc aacggctgtg tgccagcaaa ccagttcacc tggcacccgg
    13321 tatcgcgcgc ggtaggtaat gtcaaaaacc agggcgcgga gttaattcaa ccggtttgat
    13381 aagccaggag actattgatc aataatactg cgtcataaga aatctctatt agacaaagta
    13441 gacgctcctt ccacatgcgc cacaagaaac ctctattcca gtgacacaat tacgcctgat
    13501 tatttcccta gcccggaata tgaataaaaa catcaacaaa gattaaatta ttcaaacgtt
    13561 actgttgcgt ctgcggtcgt aacaacatcc gacgtggtga tgtgtatcaa atgaggcctc
    13621 tgatgatcag agggtttatt gcgtatacgg atcgtggatg gcagaaaact ttcaggatca
    13681 ggtatccatt ttaaaccaga aatagggtga gtttgcccca tccatgcccc acgcgggcag
    13741 atgggatgta attaacaaca ggttacaaaa cctacacttg catggacatc acttcctgat
    13801 acgccgccac cagcttatta cgcacctgaa tccccatttg catagaaact gaggcttttt
    13861 gcatatcggt catcacatcg tttaacgcca cgccgggttc accgagggtg aatttttctg
    13921 cctgcgtgcg ggcagctgtt tgtgtatcgc taatgcgatc gagcgcggcg tgcagctgcc
    13981 cggcaaaact aatggtcggt tgcggcagtg aatcctgcgc gcgcgcactc atcgccgtag
    14041 cctgtaactg gctgataacc ccttcaatcc cctgtatcgc tgacattttc atctcctgga
    14101 taatttctgg tagcaaagcc taccagtaag tcaataagac aaaggcgcta aatagcaaca
    14161 aaaaaacggg tttattggcg gatagaaaaa aacgaaagca caaataatgg gagcgtcaat
    14221 ttttcgagtt tgctgacccg ggagtgagtc ttgttccact ttgccaataa cgccgtccat
    14281 aatcagccac gaggtgcgcg atgaatgcga ctgcagccca gacaaaatct cttgagtggc
    14341 ttaatcgcct gcgtgcgaat ccgaaaattc cattgattgt tgccggttcc gcggcagtgg
    14401 cggtcatggt cgcactgatc ctgtgggcga aagcccccga ctaccgcaca ttattcagca
    14461 atctttccga tcaggatggt ggcgcaattg tcagccaact gacgcaaatg aatattcctt
    14521 accgcttcag cgaagccagc ggcgctattg aagttccggc agataaagtt cacgaactgc
    14581 gtctgcgcct ggcacaacaa ggtttgccca aaggcggcgc ggtcggtttc gaactactcg
    14641 atcaggaaaa gtttggtatc agccagttca gcgaacaggt gaattatcag cgggcgctgg
    14701 aaggcgagct ttctcgtacc atcgcaacta tcggcccggt aaaaggggcg cgcgtacatc
    14761 tggcaatgcc gaaaccgtct ttattcgtcc gtgaacaaaa atccccttct gcatcggtga
    14821 cggtaaatct gttacccggc cgcgcactcg atgaagggca aattagcgcc attgtgcatc
    14881 tggtttccag cgccgttgct ggtctgccgc cgggaaacgt cacgctggtg gatcagggcg
    14941 gacatctgtt aacccagtcc aataccagcg ggcgcgatct taatgatgct cagttgaaat
    15001 atgccagcga tgtcgaaggc cgtattcagc ggcgtattga agcaatcctg tcgcctattg
    15061 ttggtaacgg taatattcac gcccaggtca cggcgcagct ggacttcgcc agtaaagaac
    15121 aaacggaaga acagtatcgc cctaacggtg atgaatctca tgcggcgctt cgttcacgcc
    15181 agcttaatga gagcgagcaa agcggttccg gttatccggg cggcgtaccg ggggcgttgt
    15241 cgaatcaacc ggcacctgcg aataacgcgc caatcagcac gcctccagca aatcaaaata
    15301 accgccagca gcaggcgagc accaccagca atagcgggcc gcgtagcaca cagcggaatg
    15361 aaaccagtaa ctacgaagtc gatcgcacca ttcgtcatac caaaatgaac gtgggcgatg
    15421 tgcaacgtct gtcagtcgcg gtagtggtga attacaaaac cttgccagac ggtaaaccat
    15481 tgcctctcag caacgaacag atgatgcaaa ttgaagatct gacccgcgag gcgatgggct
    15541 tttctgaaaa acgcggtgac tcgctcaatg tcgttaactc gccgttcaat agcagtgacg
    15601 aaagcggcgg agaactgccg ttctggcaac agcaagcgtt tatcgatcag ttgctggctg
    15661 ccggtcgctg gttgctggta ctgctggtgg cgtggctgct gtggcggaaa gcggtacgcc
    15721 cgcagttaac acgtcgcgcc gaggcgatga aagctgtaca gcaacaggcg caggcccgtg
    15781 aggaagtgga agatgcggtg gaagtccgcc tgagcaaaga cgaacaactc caacaacgac
    15841 gcgctaacca acgtctgggg gcagaagtca tgagccagcg tatccgtgaa atgtctgata
    15901 acgatccgcg cgtggtggcg ctggtcattc gccagtggat aaataacgat catgagtaac
    15961 ctgacaggca ccgataaaag cgtcatcctg ctgatgacca ttggcgaaga ccgggcggca
    16021 gaggtgttca agcacctctc ccagcgcgaa gtgcaaaccc tgagcgctgc aatggcgaac
    16081 gtcacgcaga tctccaacaa gcagctaacc gatgtgctgg cggagtttga gcaagaagct
    16141 gaacagtttg ccgcactgaa tatcaacgcc aacgattatc tgcgttcggt attggtcaaa
    16201 gctctgggtg aggaacgtgc cgccagcctg ctggaagata ttctcgaaac tcgcgatacc
    16261 gccagcggta ttgaaacgct caactttatg gagccgcaga gtgccgccga tctgattcgc
    16321 gatgagcatc cgcaaattat cgccaccatt ctggtgcatc tgaagcgcgc ccaggccgcc
    16381 gatattctgg cgttgttcga tgaacgtctg cgccacgacg tgatgttgcg tatcgccacc
    16441 tttggcggcg tgcagccagc cgcgctggcg gaactgaccg aagtactgaa tggcttgctc
    16501 gacggtcaga atctcaagcg cagcaaaatg ggcggcgtga gaacggcagc cgaaattatc
    16561 aacctgatga aaactcagca ggaagaagcc gttattaccg ccgtgcgcga attcgacggc
    16621 gagctggcgc aaaaaattat cgacgagatg ttcctgttcg aaaatctggt ggatgtcgac
    16681 gatcgcagca ttcagcgtct gttgcaggaa gtggattccg aatcgctgtt gatcgcgctg
    16741 aaaggggccg agcagccact gcgcgagaaa ttcctgcgca atatgtcgca gcgtgccgcc
    16801 gatattctgc gcgacgatct cgccaaccgt ggtccggtgc gtctgtcgca ggtggaaaac
    16861 gaacagaaag cgattctgct gattgtgcgc cgccttgccg aaactggcga gatggtaatt
    16921 ggcagcggcg aggataccta tgtctgataa tctgccgtgg aaaacctgga cgccggacga
    16981 tctcgcgcca ccacaggcag agtttgtgcc catggtcgag ccggaagaaa ccatcattga
    17041 agaggccgaa cccagccttg agcagcaact ggcgcaactg caaatgcagg cccatgagca
    17101 aggttatcag gcggggattg ccgaaggtcg ccagcaaggt catgagcagg gctatcagga
    17161 aggactggcc cgggggctgg agcaaggtct ggcagaggcg aagtctcaac aagcgccaat
    17221 tcatgcccgg atgcagcaac tggtcagcga atttcaaact acccttgatg cgcttgatag
    17281 tgtgattgct tcgcgcctga tgcagatggc gctggaggcg gcacgtcagg tcatcggtca
    17341 gacgccaaca gtggataact cggcactgat caaacagatc caacagttgt tgcagcaaga
    17401 accgttattc agcggtaaac cacagctgcg cgtgcacccg gatgatctgc aacgtgtgga
    17461 tgatatgctc ggcgcaacct taagtttgca tggctggcgc ttgcggggcg atcccacact
    17521 ccatcctggc ggctgtaaag tctccgccga tgaaggcgat ctcgacgcca gtgtcgccac
    17581 tcgctggcaa gaactctgcc gtctggcagc accaggagtg gtgtaatgac cacgcgcctg
    17641 actcgctggc taaccacgct ggataacttt gaagccaaaa tggcgcagtt gcctgcggta
    17701 cgtcgctatg ggcgattaac ccgcgctacc gggctggtgc tggaagccac cggattacaa
    17761 ttgccgctcg gcgcaacctg tgtcattgaa cgccagaacg gcagcgaaac gcacgaagta
    17821 gaaagcgaag tcgttggctt taacggtcaa cggctgtttt taatgccgct ggaggaagtc
    17881 gaaggtgtcc tgcccggcgc gcgggtttat gccaaaaaca tttcggctga aggactgcaa
    17941 agcggcaagc agttgccgct cggtccggcg ttattaggtc gcgttctgga cggcagcggt
    18001 aagccgctcg atggcctgcc ctcccccgat acgacggaaa ccggtgcgct gattaccccg
    18061 ccatttaacc cgttgcaacg tacaccgatt gaacatgtgc tggacaccgg cgtgcgccca
    18121 atcaatgccc tgcttaccgt tgggcgtggg cagcgtatgg gactgtttgc cgggtccggc
    18181 gttggtaaaa gtgtgctgct ggggatgatg gcacgttaca cccgcgccga tgtcattgtc
    18241 gtgggtttga ttggtgaacg cggacgcgaa gtaaaagatt ttattgagaa tatcctcggt
    18301 gccgaagggc gtgcacgctc cgtggtgatt gccgctccgg cggatgtttc tcctctcctg
    18361 cgaatgcagg gtgccgccta tgccacgcgc attgccgaag attttcgcga tcgtggtcag
    18421 catgtgttgc tgattatgga ctccctcacc cgctacgcga tggcccagcg tgaaattgcg
    18481 ctggcgattg gcgaaccacc cgccactaaa ggttatcccc cgtcggtgtt tgccaaatta
    18541 ccggcactgg tcgagcgtgc cggaaatggc attagcggcg gcggatcgat tactgcattt
    18601 tataccgtgc tcactgaagg cgatgaccag caggacccca ttgccgactc tgcgcgggca
    18661 atcctcgacg gtcacattgt gctgtctcgc cgactggcgg aagccgggca ctatccggct
    18721 atcgatattg aagcgtcgat cagccgcgca atgacggcgt tgatcagtga gcaacattac
    18781 gcgcgagtgc gcaccttcaa acagctgttg tcgagttttc agcgtaaccg cgatctggtt
    18841 agcgtcggcg cgtatgccaa aggcagcgat ccgatgctcg acaaagccat cgccctgtgg
    18901 ccgcagctgg agggctattt gcaacaaggc atttttgaac gcgcggactg ggaagcgtct
    18961 ctccaggggc tggagcgtat tttcccgaca gtgtcataac ccaggagata acggcagatg
    19021 gcagaacatg gcgcgctggc gaccctgaaa gatctggcag aaaaagaggt agaggatgcc
    19081 gcgcgcctgc tgggtgaaat gcgtcgcggg tgtcagcagg cggaagaaca gctcaaaatg
    19141 ctgatcgatt atcagaatga atatcgcaat aacctcaaca gcgatatgag tgccgggatg
    19201 accagcaacc gctggatcaa ctatcagcag tttatccaga cgctggaaaa agccattact
    19261 cagcatcgcc agcaacttaa tcagtggacg cagaaagttg acattgccct gaacagctgg
    19321 cgagaaaaga aacaacgttt gcaggcctgg cagacactgc aggaacggca atccacggcg
    19381 gcactgcttg cagaaaaccg cctcgatcag aaaaagatgg atgagttcgc ccagcgcgcc
    19441 gccatgagga aacctgaatg attcgcttag cgcccttaat taccgccgac gttgacacca
    19501 ccacattgcc tggcggcaaa gccagcgatg ctgcacaaga ttttctcgcg ttgttgagcg
    19561 aagcattagc aggcgagaca actaccgaca aagcggcccc ccagttgctg gtggcaacag
    19621 ataagcccac gacaaaaggc gagctgctga tcagcgatat tgtttccgac gcgcaacaag
    19681 cggatttact gatccctgtg gatgaaacac tgcctgtcat caacgtcgaa caatccacat
    19741 caacaccgtt aaccaccgct cagacgatga cgttggctgc ggtggctgac aaaaatacga
    19801 caaaagacga aaaagcggat gatctgaatg aagacgtcac cgcaagcctg agcgcccttt
    19861 ttgcgatgtt gccgggtttt gacaatacgc ccaaagtgac tgatgtgccg tcaaccgtgt
    19921 taccggcaga gaaaccaacg ctcttcacaa aactgacttc tgcgcaactc acaacagcac
    19981 agcctgatga tgcccccggc acgccagctc agccattaac accgctggta gcagaagccc
    20041 agagtaaagc ggaagtcatc agcacaccgt caccggtgac cgctgccgcc agcccgctaa
    20101 ttactccaca ccagacacag ccactgccca ccgtcgccgc gcctgttttg agtgcaccgc
    20161 tgggttctca cgaatggcaa caatcattaa gccagcatat ttcgctattc acccgccagg
    20221 ggcaacaaag tgcagagttg cgtctgcacc cgcaggattt aggtgaagtg caaatctccc
    20281 tcaaagtgga tgataaccag gcacaaatcc agatggtttc accgcatcag catgtacgcg
    20341 ccgcgctgga agcagcgctt ccggtactgc gtacgcagct ggccgaaagt ggcattcagt
    20401 tagggcaaag caacatcagt ggcgaaagct ttagtggtca gcagcaggcc gcttcccaac
    20461 aacagcaaag ccaacgcaca gtaaaccatg aacctctggc gggggaagac gacgatacgc
    20521 ttccggttcc cgtctctata caagggcgcg taacaggcaa cagcggcgtt gatattttcg
    20581 cctaacgtca gaggtagcac cgtaatccgc gtcttttccc cgctttgttg cgctcaagac
    20641 gcaggataat tagccgataa gcagtagcga cacaggaaga ccgcaacaca tgactgatta
    20701 cgcgataagc aagaaaagca agcgatcgct ttggatcccg attctggtat tcattaccct
    20761 cgcggcctgt gccagcgcag gttacagcta ctggcattcg catcaggttg ccgctgacga
    20821 caaagcgcag caacgcgtcg taccctcccc ggtcttctac gcgctggata ccttcacggt
    20881 caatctgggc gatgcggatc gcgtgcttta tatcggcgta accctgcgcc tgaaagatga
    20941 agctacccgc tcgcggctga gtgagtattt gccggaagtc cgtagccgtt tgctgttact
    21001 gttttcgcgt caggatgctg ccgtactggc gacagaagaa ggcaagaaaa acctgattgc
    21061 cgagattaaa accacgcttt ccaccccgct tgttgccggg caaccgaaac aggatgtcac
    21121 cgacgtgctg tataccgctt ttattctgcg ataacgacat gggcgatagt attctttctc
    21181 aagctgaaat tgatgcgctg ttgaatggtg acagcgaagt caaagacgaa ccgacagcca
    21241 gtgttagcgg cgaaagtgac attcgtccgt acgatccgaa tacccaacga cgggttgtgc
    21301 gcgaacgttt gcaggcgctg gaaatcatta atgagcgctt tgcccgccat tttcgtatgg
    21361 ggctgttcaa cctgctgcgt cgtagcccgg atataaccgt cggggccatc cgcattcagc
    21421 cgtaccatga atttgcccgc aacctgccgg tgccgaccaa cctgaacctt atccatctga
    21481 aaccgctgcg cggcactggg ctggtggtgt tctcaccgag tctggtgttt atcgccgtgg
    21541 ataacctgtt tggcggcgat ggacgcttcc cgaccaaagt ggaaggtcgc gagtttaccc
    21601 ataccgaaca gcgcgtcatc aaccgaatgt tgaaactggc gcttgaaggc tatagcgacg
    21661 catggaaggc gattaatccg ctggaagttg agtacgtgcg ttcggaaatg caggtgaaat
    21721 ttaccaatat caccacctcg ccgaacgaca ttgtggttaa cacgccgttc catgtggaga
    21781 ttggcaacct gaccggcgaa tttaatatct gcctgccatt cagcatgatc gagccgctac
    21841 gggaattgtt ggttaacccg ccgctggaaa actcgcgtaa tgaagatcag aactggcgcg
    21901 ataacctggt gcgccaggtg cagcattcac agctggagct ggtcgccaac tttgccgata
    21961 tctcgctacg cctgtcgcag attttaaaac tgaaacccgg cgacgtcctg ccgatagaaa
    22021 aacccgatcg catcatcgcc catgttgacg gcgtcccggt gctgaccagt cagtacggca
    22081 ctctcaacgg tcaatatgcg ttacggatag aacatttgat taacccgatt ttaaattctc
    22141 tgaacgagga acagcccaaa tgagtgacat gaataatccg gccgatgaca acaacggcgc
    22201 aatggacgat ctgtgggctg aagcgttgag cgaacaaaaa tcaaccagca gcaaaagcgc
    22261 tgccgacgcg gtgttccagc aatttggcgg tggtgatgtc agcggaacgt tgcaggatat
    22321 cgacctgata atggatattc cggtcaagct gaccgtcgag ctgggccgca cacgaatgac
    22381 catcaaagag ctgttgcgtc tgacgcaagg gtccgtcgtg gcgctggacg gtctggcggg
    22441 cgaaccactg gatattctga tcaacggtta tttaattgcc cagggcgaag tggtggtcgt
    22501 tgccgataaa tatggcgtgc ggatcaccga tatcattact ccgtctgagc gaatgcgccg
    22561 cctgagccgt tagtgatgaa taaccacgct actgtgcaat cttccgcgcc ggtttctgct
    22621 gcgccactgc tgcaggtgag cggcgcactc atcgccatta ttgccctgat cctcgctgct
    22681 gcctggctgg taaaacggtt gggatttgtc cctaaacgca ctggcgttaa cggtctgaaa
    22741 attagcgcca gtgcttcgct gggcgcgcgt gaacgggttg tggtggtcga tgtggaagat
    22801 gcacggctgg tgctcggcgt taccgcaggg caaatcaatc tgctgcataa acttccccct
    22861 tcagcaccaa cggaagagat accgcagccc gattttcagt cggtcatgaa aaacttgctt
    22921 aagcgtagcg ggagatcctg atgcgtcgtt tgttgtctgt cgcccctgtc cttctctggc
    22981 tgattacgcc cctcgccttc gcgcaactgc cgggtatcac cagccagccg ctgcctggcg
    23041 gtggacaaag ctggtcgctc ccggtgcaga cgctggtgtt catcacctcg ttgacgttta
    23101 ttccggcaat tttactgatg atgaccagtt tcacccgcat cattattgtt tttggtttat
    23161 tgcgtaacgc gctgggaaca ccctccgcgc cacctaacca ggtattgctg gggttggcgc
    23221 tgtttttgac cttttttatt atgtcgccgg tgatcgacaa aatttatgtc gatgcgtacc
    23281 agccattcag cgaagagaaa atatcaatgc aggaggcgct ggaaaaaggg gcgcagccgc
    23341 tgcgtgagtt tatgctgcgt cagacccgtg aggcggattt agggttgttt gccagactgg
    23401 cgaatactgg cccgttgcag ggacctgaag ccgtgccgat gcgcattttg ctcccggcct
    23461 acgtgaccag cgagttgaaa accgcatttc agataggctt cacgattttc atcccttttt
    23521 tgattatcga cctggtgatt gccagcgtgt tgatggcatt ggggatgatg atggtccccc
    23581 ccgccaccat tgctctgccc tttaaactga tgctgtttgt actggtggat ggctggcaat
    23641 tgctggtcgg ttcgctggcg cagagctttt acagctagag aggcaaaatg acacctgaat
    23701 cggtcatgat gatggggact gaagcgatga aagtcgcgct ggcactggct gccccgctgt
    23761 tgctggtggc gctggtcacg ggccttatca tcagtatttt gcaggccgcc acgcagatca
    23821 acgaaatgac gctgtcgttt attccaaaaa tcatcgccgt atttattgcc attattattg
    23881 ccggaccgtg gatgctcaat ctgttgctgg attacgtccg caccttgttc actaacctgc
    23941 cgtatatcat cgggtagccc gtacaatgat gcaggtgaca agcgatcaat ggctttcctg
    24001 gctaagtctg tacttctggc cgttactgcg cgtgctggcg ctcatttcta ccgcgccaat
    24061 tctgagcgaa cgcagcgtgc cgaaacgggt aaaactgggt ctggcaatga tgatcacgtt
    24121 cgctattgcc ccatcattac ctgccaacga tgttcctgtt ttttcgttct ttgctctgtg
    24181 gctggccgtg cagcagatcc tgatcggcat tgcgcttggt ttcaccatgc aatttgcctt
    24241 tgccgctgtg cgaaccgctg gcgaaattat cggtctgcaa atggggctgt catttgcgac
    24301 gtttgtcgat ccggccagcc atctcaacat gcccgttcta gcgcgtatca tggatatgct
    24361 ggcgttactg ctgttcctga catttaacgg tcatttatgg ttgatttcac tgctggtcga
    24421 tacctttcac accctgccga ttggtggcga accgttgaac agcaatgcgt ttctggcact
    24481 caccaaagca gggagtttga ttttccttaa cgggctgatg ctggcgttac cgctcattac
    24541 tctgctgctg accctgaatc tggcattagg tttactgaat cgtatggccc cgcaattatc
    24601 catttttgtt attggatttc cattaactct gactgtcggt atctctttaa tggcggcatt
    24661 aatgccgtta attgcgcctt tttgcgaaca tttattcagt gagatgttta atttgctggc
    24721 tgatattatt agtgaattgc cattaatata attccgtaac gtttatcatg ttatcctaag
    24781 gattatccga aaaataatac ctacgaacat cttccaggat actcctgcgg cgaaatattt
    24841 gttttaagct cactcacata tcgcaacatt tactttactt taagacaatt ccaggcaaat
    24901 tatacaacac tttacgggat agtaagtccg cctgaaaaat cgcgagagtg gcgcattagg
    24961 tgacccatgt tgttccgttt agtcatgatg aaatattcag gtaaggggaa ttatcgttac
    25021 gcattgagtg agggtatgcc atgtcaacga ttattatgga tttatgtagt tacacccgac
    25081 taggtttaac cggatatctg ttgagtagag gggttaaaaa aagagaaatc aacgacattg
    25141 aaaccgttga tgaccttgcc atagcttgtg attcacagcg cccttcagtg gtgtttatta
    25201 atgaggactg tttcatccat gatgcttcta acagtcagca catcaagcac atcattaatc
    25261 aacatcccaa tacgttattt atcgttttta tggcaattgc caatgttcat tttgatgaat
    25321 atctattggt cagaaaaaat ttattgataa gttctaaatc gattaaaccg gaatctctcg
    25381 acgatatcct tggcgatatt ctgaaaaaag agacaacgat aacctctttt ttaaatatgc
    25441 cgacgttatc attgagccga accgaatcga gtatgttgcg aatgtggatg gcaggtcagg
    25501 gaacgattca aatctctgac caaatgaata tcaaagccaa gaccgtttca tcgcataaag
    25561 gtaatattaa acgtaagatc aaaacgcata ataaacaggt tatctaccat gtcgtccgac
    25621 tgacggataa tgtgactaat ggtatttttg tcaacatgcg ctaacacatt ctgactggtg
    25681 gtttcccacc agtcaggctg aataagatta ctctgctttc tccacaaaga taccgtcctg
    25741 atgccctgct tcattaaaga accagatgcc gagcgggtag tcttccagcg aaaccaggta
    25801 cattgtgcct tcactaaact cctcaactgc cagtaccacg ccaggacgac gcggaccgcc
    25861 atccgttttg actgttaccc gatcattcac cttcatcgtt ttcctcctgt ggctttgtgc
    25921 cagtgtagaa caatttcgtt tttctggcag cgccaggcgc gcgcgagtgc tgattttctc
    25981 gacggtctat acttaagaga tgccaggcgg acttaacgac tggcggcaac aacagagtaa
    26041 cggttgcgag gaaagatgat gaaaaccgca aaagagtaca gcgataccgc aaaacgtgag
    26101 gtcagcgtcg atgtcgatgc cctgctggcg gcgatcaatg aaattagcga aagcgaagtt
    26161 catcgcagcc agaacgattc tgaacacgtt agcgtcgatg gacgtgaata tcatacatgg
    26221 cgtgaattgg cggatgcctt cgaactggat attcatgact tcagcgtctc tgaagtgaat
    26281 cgttgaatgc acaataaaaa aatcccgacc ctgagggggt cgggatgaaa cttgcttaag
    26341 caagaagcac ttaaaaaatt cgttacacca ggaaatctga tgtgttcatc accttatccg
    26401 caattttttt cgctgacaag aaaatattcg cgatatgaat gattaacgct acttatgaat
    26461 agaaatgtga cgtgcgtcac cccttggtgg ctctggcgtc aacgggatcg ctcccgaaaa
    26521 aatcatcttt gattatttgt gctgttttga gaatcatcct ggtaagggga gaactgcgcg
    26581 accatgtttt caattcaaca accactactg gtttttagcg atcttgatgg caccctgctg
    26641 gacagtcata gttatgactg gcaaccagct gccccctggc tcagccgttt acgcgaagcg
    26701 aatgttcccg tcattctctg tagcagtaag acatcagcgg aaatgttgta cttgcaaaaa
    26761 acgttggggc tacaaggttt accgctgatt gccgaaaatg gcgcagtgat ccagcttgct
    26821 gagcaatggc aggatataga cggttttcca cgcatcatct caggtattag ccatggcgaa
    26881 atcagccagg tattaaatac gctacgcgag aaggaacatt ttaaattcac gacttttgat
    26941 gatgtcgacg atgcaaccat cgccgaatgg acgggattaa gccgtagcca ggcggcgctg
    27001 acgcagctgc atgaggcgtc ggtaacgcta atctggcgcg acagtgacga gcgtatggca
    27061 caatttaccg ctcgtctgaa cgaactgggc ttacagttta tgcagggtgc gcgcttctgg
    27121 cacgtcctgg atgcctctgc cggaaaagat caggccgcca actggattat cgcgacctat
    27181 caacaattat caggcaaacg cccaaccaca cttggcctgg gcgatgggcc aaacgatgcg
    27241 cccttactgg aggtaatgga ttacgcggtg attgtgaaag ggctaaaccg tgaaggggtg
    27301 catctgcatg atgaggatcc gacccgcgtc tggcgaacgc agcgtgaagg accggaaggc
    27361 tggcgtgaag ggctggacca ttttttctcc gctcgttaag cgttatcgct cgcgcatacc
    27421 cgattacgcc cggcctgttt agcgagataa agccgacggt cagccagtga ctggagttgt
    27481 tcaaaatcat aatcaccggt ttcctcgctg ctactaaccc ccaacgaggc actgatgcgt
    27541 atcgtcgtac tcttagcaat caacatctct ttttcattta acttcaggcg aatacgttct
    27601 gcgacttccg cagcctccgt cagactcgcg cctggcagaa tcacacaaaa ctcctcacca
    27661 ccgacccgcc cggcaacgtc ctgcgcacgc aaggaactgc taattaatcc ggcagcatga
    27721 gaaagaacac ggtcgcccgc ctgatgacca aagcggtcat taatcgcttt aaaatggtca
    27781 agatcgacct gaatgacaga aaaaggatgt tggtgcgtct gacacaattt agcgagcgga
    27841 cgggcttttt cgaacagtgc gccacgatta tataaacggg ttaaggtgtc gtgccacgcc
    27901 tgccactgca acgagctttg cagaacatac atgttgctga ccatccggcg aatcacatac
    27961 caggagatga gtaacatggt ggtaaagagc gcccacagca gggttaatgc aatgctgata
    28021 ctaccgaaat cgccgcgcac gccttcgctt agcgtatgga cgcgcaccag cacaccgtcg
    28081 aaatggtcca gacgttccca gctaacatag cgactgtcca tacgaatgcc gccccgcgtg
    28141 tcatgttcca tcgcctgcgc cagcaaggcc agttcacgag gatcaaaaat attccctgtt
    28201 ggatggtcag gattggaaga ggtcaaaaat ctcagcttac tgtcatagag ctgatactca
    28261 ccatcgaggt ttttatcgat ggcgtttctt aaaaattgct gcatagtacg cacaggaata
    28321 ctcatcccca gcacgccata ccagtagtta ttactgtcta ccggaacact gacggtaacc
    28381 tgcggttcag tattgctggc gtgttccggt tgcgaagtaa accagcgtac cgcgcggtga
    28441 cgattttctc gttgcgaatg gccgataaac cagggttggg tgacatagcc gtaataacgc
    28501 gttggaacat tgcgcgtaaa caaggtcggc tgcgtcgaaa cgtaaaatcc ggcacgcgag
    28561 acatacatcg cctgttcaac catcgacgag gagttgtgcg ccagtcgcag caagtaacca
    28621 acttccagtg cagcggtaat ttcattgtcg aggctttcat tttcgcgaga caggagattc
    28681 ccctcgctga ctaatgcatc cgacacacca ttaaatggca gagtgcgccg tcggttgagt
    28741 tcaatttgcc aggcgtgctc gtcgcgatgc tgttcgaact cggttacagc attacgcaga
    28801 gaggtgaaat ccagtggcgc tacgagagct tcgcgcatgc cattacgcaa aaagatcagt
    28861 ttgtcgacgt tatactgcaa atgcttatcg agcgcgttgg ctacgttttc cagatgatta
    28921 cgctggctgg agatataggc atcttccagc accactactt cacgccaggt gagcaaggtg
    28981 gaaaaaagca ataccacgat aaagcagaga ttaacgacat gaccaggccc caggcggcgt
    29041 gcgagttttt tcaaccagct ctggttttcc atttttgtct cgtgctgcac cctgacgact
    29101 cccaacctgc ttttttatga ttctggcata acctggcgat agcgtaccgc aaaacagcga
    29161 taattttata atggtggcat gataagacgc gactatcatt cattgcaggc attggcacac
    29221 caggccggat gtgatccggc ctggtgttaa tgataccctt tcaaacttct tccgcagaac
    29281 gactcccctc ccccggaaca agctcggttt catgaaaagc ttcccgcttg accccgtaac
    29341 cgtcatacca acgacactca accataccgc tggagtatcc ggtgacaatc atccgcgggc
    29401 caccctcttt aaccgtaact tcctcactaa ccataaagct catactcgcc tccttttttc
    29461 gagtgaaact tgttcacctt agttgaagat ggcgaatttt gcataatagc ctgtgcgagt
    29521 attaatgtgc ctgaccgcgc attttcgcta cggctttcac tcccagcacc acgatgccgc
    29581 cgatgataaa tccaagaatc agatttaaaa cagtcggtaa tatcatcgcc accactgcac
    29641 tttgctgccc ggcgaaatgt tcaatggcgt gatgcagcgg cgcaatacca tgtaccacaa
    29701 tcccgccgcc gacgaggaac atcgccagcg tgccgacaat cgataacgcc ttcatcaacc
    29761 agggcgcaat gatcaataac cctttaccta atgcctgcat cagcgcgctg gatttttccg
    29821 ccagccaata ccccaggtca tcaatcttaa cgataacccc taccagaccg tagacgccca
    29881 cggtcactac cagcgcgatg cctgaaagca ccagcacctg attaagcaac ggcgcttcgg
    29941 ccacaatccc cagcgtgatg gcgacgattt cggcagacaa tataaaatcg gtacgaatcg
    30001 ccccttttat tttgtccttt tcaaacttca gcggatcctg cgccgccagc ttctccagac
    30061 gctgctggct ctgcgccgga tcttctttat gtttacgcgc ctccagcata tgcagcactt
    30121 tctctactcc ttcaaagcag agaaacgcgc caccaatcat caacagaggc gtaatcgccc
    30181 acgggataaa cgcactgatg atcagcgcca gcggcaccag aatcacttta ttaatcagcg
    30241 atcctttcgc cacgccccag accacgggaa gttcccggtt ggcccgcacg cctgaaactt
    30301 gttgcgcatt gagcgataag tcatccccta atacaccggc ggttttcttc gccgccagtt
    30361 tgcccatcac ggagatatcg tccagtagtg tggcgatatc atcgagcaac gtcagtaaac
    30421 tactcccggc caaaagcatt ccctctcatt tttattgttg aagggtgtaa gtatgaagca
    30481 aaaacggcag ctctgatact tgcttcacag gtcaacattt ttttaacaat taagtgcaat
    30541 tatatctctc gccagcagaa tgattttcac gtttactatg aaccaccttt ttatttccac
    30601 cgtgagggat tatgcgtttc cgccagttgt taccgctttt tggcgcgctg tttgcgttgt
    30661 atatcatttg gggctcaacc tattttgtca ttcggattgg cgtggaaagc tggcctccgt
    30721 taatgatggc aggcgttcga ttcctggcag ccggtatttt attgatggca tttttgctac
    30781 tgcgcggaca caagctcccc ccgctacgtc cgctgctcaa tgccgcgctg attggcctgt
    30841 tattgctggc tgtcggtaat ggcatggtga cggttgccga gcatcaaaat gttccttccg
    30901 gcatcgccgc cgttgtggtt gcaaccgtgc ccctctttac cctgtgcttc agccgcctgt
    30961 ttggcattaa aacgcgcaaa ctggaatggg tgggtatcgc cattgggctt gccggaatca
    31021 tcatgctcaa tagcggtgga aatttaagcg gcaatccgtg gggcgcgatt ctgattttaa
    31081 tcggctcgat tagctgggcg tttggctcgg tttatggctc gcgcattacc ttacctgtag
    31141 ggatgatggc gggtgctatt gagatgctgg cggcaggcgt ggtgttaatg atcgcgtcga
    31201 tgattgcagg tgaaaaactg acggcgctcc cttccctttc aggcttcctt gcggtcggct
    31261 atctggcgct gtttggttcg attatcgcca tcaacgctta tatgtattta atccgtaatg
    31321 tcagtccggc tctcgccacc agctacgcct acgttaaccc ggtggtcgcg gtcttgctgg
    31381 gtacgggact gggcggagag acgctgtcga agattgaatg gctggcgctc ggcgtaattg
    31441 tcttcgcggt ggtgctggtc acgttgggaa aatatctctt tccggcaaaa cccgtagttg
    31501 cgccagttat tcaggacgca tcaagcgagt aaatgaatcc cctgcgtgtc gatctgcgcg
    31561 ctggcacctt cgccgcagat ccactcttcc agacgctcgc taagcgcttc atccgtcagc
    31621 ttctcgcgcc cacgtaacgc gcactcccag acaatcaata cgcgccagcc gagttcctgc
    31681 aagcgactga tatcgcggcg atcgcgctca acatttttac ctatcttctc cagccagaac
    31741 tcggttcgag tcgcaggcac tttaaacaga tagcagtgat gatgatgcca gaagcagccg
    31801 tgggtaaata tcacgcagcg atattcatca acgacaaaat ccgggcgtcc gggcagactg
    31861 gcgtcctgaa cgcgaaacgc caggccctgc ccggttaaca gactggcgag gcgcttctct
    31921 atcgccgtat cacgcgtggc aatcgcgcgc atatttttgc tgcgagtggc cttatcgtga
    31981 acgtcggcca tgttgtgcct cttgctgacg caacgccacc gcctgtttga tttttggctc
    32041 aagcagtttt gccaccgcgg caaagaccgg cacgaccacc gagttaccga actggcgata
    32101 ggcctgagtg tccgaaaccg gaatgcggaa tttcgcttct cccggcgctt caaaacccat
    32161 taagcgcgcg cattcacgag gcgttaaccg acgtggacga tgttgctgat tctgcggatc
    32221 gtcaaagtct ttctcacctt tggccatatc ccagccgcga tcgattaaaa tttccgcgcc
    32281 atctttgtaa taacgcgcag acagcgtacg cgtgacgctt tgcggattgt tcggataaac
    32341 cattccataa ccgaagccgt taccgcgcgc ctgatgtttt ttcgcatatc gatagaggta
    32401 cttccacagc accggcgtca ggatatattt cgcctcgacc atcgggtcca gcagctgcgc
    32461 cagcgtcact cgctgcgcag ggaaacattc gctgatatca cgcagggtaa aatcggcttt
    32521 cagattcaga tcgcgacgaa aacccaccag cacgatgcgt tcacggtgct gtggcagaaa
    32581 atgtttgccg tcgatgattt tcggatcgtc tggcccgtta tcttctgcat cagcctcgtc
    32641 atagcccagt tcgtccagcg tctgcatgat gatgcggaac gttttaccct ggtcatggct
    32701 tttcaggttt ttgacgtttt cgagcacaaa catcgccgga cgacgcgagt cgataatgcg
    32761 caccacatca aaaaacagcg tgccctgggt atcgcaggca aaaccgtgcg cccgcccgag
    32821 cgagttcttt ttcgatacgc cagccagcga aaatggctga caagggaaac cggccagtaa
    32881 aacatcgtgt tcaggaatgt gttgacgaat atgttccgcc gccgcctcat cactcacgcc
    32941 ttctttatgg ctgagggtga tgtcgcggat atcttcatta aaatgatgcg tcgccgggtc
    33001 gcaataatga ttggctttat aagtgcgtac cgcatgtttg ttccattcgc tggtaaacac
    33061 gcactgtccg ccaatcgatt caaaaccgcg acggatgccg ccaattccgg caaatagatc
    33121 gataaagcga aacgcataat gcggatgatg tgccggtggt ttgggtaaca acgtttgcag
    33181 atgggcgaac tctttctcac ttaaacggtg ccatgccgag tcattcgcca gcgcacgttt
    33241 taaaattgcc gcgctccagt gattctcacc cacaccatta agctgcgcca ccagcgtttt
    33301 aacatcataa atttgcagca gtttctccag cattgcctgt gcggcattcc cggtgctgta
    33361 tgaatcggtt actgatatat tttcctgcat agatttaacc ggccatctga gatggccgac
    33421 aggttaacat aattacagcc atttagggcc acgcgcttat cccgcgggtg aaaacgcatc
    33481 tatcaccttg tgatcgacac cttcattctc ccccgccagt tcggcactga gcttcgccat
    33541 aaactcgact aaaaaatgcg cgttgtgctg cgccagctgc ttgcctcttg cggtttgcat
    33601 ggtttgcggt agcttcagca acttagtctg gaaatgatcc agcgcgtagc gtttatcatc
    33661 aagcgggcga tgctgtgcga acggatcttc accatcaaac agcgccacgc ccaatgcccc
    33721 tgaaacggca aacacgcgcg ccaggccaat cgcccccagg gcttccagcc gatccgcatc
    33781 ctggacgatt ttagcttccg ttgttaaggg ggcgatttgc gcgctgaaac tgtgagcggc
    33841 aatggcatga caaacggcct cgattttctc cgccggaaat tgctcgaact cttcacggag
    33901 caggcgacgc gtctcttctg ccgccaggat tgaagaacgc tgcctttgcg gatgattttt
    33961 cgccaggctc acaatatcgt gaaaataaca ggcggttaaa atcaccagca tatcgacatc
    34021 gtcatctgcc gccagcttct gtgccgtagc ccagacgcgg cgaaaatgac acacgtcatg
    34081 ggccgcgccc tggtgctgat gatggttttt caaccagttt tcgaactgtg cctgccagtg
    34141 ttgtaagtcc atacgcgctc cgttgccaaa gagtcgccac cttagcagtt tttattcata
    34201 gcgtctgcta cgtgcgggac gaggtcgggt gtaccaggca atgctgccta acaatgcccc
    34261 gaccagagcc ataaataaaa atccgaccag tgcccctaac cactttcccg cggtagagcc
    34321 taaatcgctt tcaaatcccg acaccggggc atcggggatc cggctcatca cccagatgta
    34381 acgcgccatt gcccagacga aatagccaca ccatgtatag aaagcccaca aagccagttt
    34441 gccaccaggg ctgcgagaga gtctctcttc catctttgtc gaaccgtatt ccattttgag
    34501 ataacccgga aaattcagac ttagcactat tatgtgataa aagtcacatt tttccacatt
    34561 ggatgagcca gtcaagactc actcattttt tgaacaaata gataattttt gcgcaacggc
    34621 agtgaataca ggaaaaatcg ttttgagcga ttttatttat ctttgagatc ggctttatta
    34681 attcattta
//
LOCUS       DAZFXT010000043        34326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.043, whole genome shotgun sequence.
ACCESSION   DAZFXT010000043 DAZFXT010000000
VERSION     DAZFXT010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.043"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..790
                     /locus_tag="ACHKW0_002185"
                     /pseudo
     CDS             1..790
                     /locus_tag="ACHKW0_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(794..2185)
                     /gene="tcyP"
                     /locus_tag="ACHKW0_002186"
     CDS             complement(794..2185)
                     /gene="tcyP"
                     /locus_tag="ACHKW0_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HFO7475019.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2318..2908)
                     /gene="ydjM"
                     /locus_tag="ACHKW0_002187"
     CDS             complement(2318..2908)
                     /gene="ydjM"
                     /locus_tag="ACHKW0_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7475020.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(3071..3739)
                     /gene="hxpB"
                     /locus_tag="ACHKW0_002188"
     CDS             complement(3071..3739)
                     /gene="hxpB"
                     /locus_tag="ACHKW0_002188"
                     /EC_number="3.1.3.22"
                     /EC_number="3.1.3.50"
                     /EC_number="3.1.3.68"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HFO7475021.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3886..4422
                     /gene="yniB"
                     /locus_tag="ACHKW0_002189"
     CDS             3886..4422
                     /gene="yniB"
                     /locus_tag="ACHKW0_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HFO7475022.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4463..5323)
                     /gene="yniA"
                     /locus_tag="ACHKW0_002190"
     CDS             complement(4463..5323)
                     /gene="yniA"
                     /locus_tag="ACHKW0_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HFO7475023.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5429..5719)
                     /gene="ghoS"
                     /locus_tag="ACHKW0_002191"
     CDS             complement(5429..5719)
                     /gene="ghoS"
                     /locus_tag="ACHKW0_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7475024.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5820..6749)
                     /gene="pfkB"
                     /locus_tag="ACHKW0_002192"
     CDS             complement(5820..6749)
                     /gene="pfkB"
                     /locus_tag="ACHKW0_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HFO7475025.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            7036..7794
                     /gene="ydiY"
                     /locus_tag="ACHKW0_002193"
     CDS             7036..7794
                     /gene="ydiY"
                     /locus_tag="ACHKW0_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HFO7475026.1"
                     /translation="MKLLKTVPAIVMLARGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRITLSLGYSM"
     gene            complement(7847..7954)
                     /gene="yniD"
                     /locus_tag="ACHKW0_002194"
     CDS             complement(7847..7954)
                     /gene="yniD"
                     /locus_tag="ACHKW0_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YniD"
                     /protein_id="HFO7475027.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(8130..10028)
                     /gene="espL1"
                     /locus_tag="ACHKW0_002195"
                     /pseudo
     CDS             complement(8130..10028)
                     /gene="espL1"
                     /locus_tag="ACHKW0_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            10552..12480
                     /gene="thrS"
                     /locus_tag="ACHKW0_002196"
     CDS             10552..12480
                     /gene="thrS"
                     /locus_tag="ACHKW0_002196"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HFO7475028.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEMFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            12484..13026
                     /gene="infC"
                     /locus_tag="ACHKW0_002197"
     CDS             12484..13026
                     /gene="infC"
                     /locus_tag="ACHKW0_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HFO7475029.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            13123..13320
                     /gene="rpmI"
                     /locus_tag="ACHKW0_002198"
     CDS             13123..13320
                     /gene="rpmI"
                     /locus_tag="ACHKW0_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HFO7475030.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            13373..13729
                     /gene="rplT"
                     /locus_tag="ACHKW0_002199"
     CDS             13373..13729
                     /gene="rplT"
                     /locus_tag="ACHKW0_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HFO7475031.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    13846..13970
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            13852..13896
                     /gene="pheM"
                     /locus_tag="ACHKW0_002200"
     CDS             13852..13896
                     /gene="pheM"
                     /locus_tag="ACHKW0_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HFO7475032.1"
                     /translation="MNAAIFRFFFYFST"
     gene            14180..15163
                     /gene="pheS"
                     /locus_tag="ACHKW0_002201"
     CDS             14180..15163
                     /gene="pheS"
                     /locus_tag="ACHKW0_002201"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HFO7475033.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            15178..17565
                     /gene="pheT"
                     /locus_tag="ACHKW0_002202"
     CDS             15178..17565
                     /gene="pheT"
                     /locus_tag="ACHKW0_002202"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HFO7475034.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHSLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGKRIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            17570..17869
                     /gene="ihfA"
                     /locus_tag="ACHKW0_002203"
     CDS             17570..17869
                     /gene="ihfA"
                     /locus_tag="ACHKW0_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HFO7475035.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            17970..18950
                     /gene="btuC"
                     /locus_tag="ACHKW0_002204"
     CDS             17970..18950
                     /gene="btuC"
                     /locus_tag="ACHKW0_002204"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HFO7475036.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            19013..19564
                     /gene="btuE"
                     /locus_tag="ACHKW0_002205"
     CDS             19013..19564
                     /gene="btuE"
                     /locus_tag="ACHKW0_002205"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HFO7475037.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            19564..20313
                     /gene="btuD"
                     /locus_tag="ACHKW0_002206"
     CDS             19564..20313
                     /gene="btuD"
                     /locus_tag="ACHKW0_002206"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015889 - cobalamin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HFO7475038.1"
                     /translation="MSIVMQLQDVAETTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            20391..20855
                     /gene="nlpC"
                     /locus_tag="ACHKW0_002207"
     CDS             20391..20855
                     /gene="nlpC"
                     /locus_tag="ACHKW0_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO7475039.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            21102..21815
                     /gene="ydiV"
                     /locus_tag="ACHKW0_002208"
     CDS             21102..21815
                     /gene="ydiV"
                     /locus_tag="ACHKW0_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HFO7475040.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQSQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            21878..23314
                     /gene="selO"
                     /locus_tag="ACHKW0_002209"
     CDS             21878..23314
                     /gene="selO"
                     /locus_tag="ACHKW0_002209"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0070733 - AMPylase activity [Evidence
                     IEA];
                     GO_process: GO:0018117 - protein adenylylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein adenylyltransferase SelO"
                     /protein_id="HFO7475041.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(23318..23509)
                     /gene="hemP"
                     /locus_tag="ACHKW0_002210"
     CDS             complement(23318..23509)
                     /gene="hemP"
                     /locus_tag="ACHKW0_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HFO7475042.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(23641..24687)
                     /gene="aroH"
                     /locus_tag="ACHKW0_002211"
     CDS             complement(23641..24687)
                     /gene="aroH"
                     /locus_tag="ACHKW0_002211"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HFO7475043.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(24844..25677)
                     /gene="ppsR"
                     /locus_tag="ACHKW0_002212"
     CDS             complement(24844..25677)
                     /gene="ppsR"
                     /locus_tag="ACHKW0_002212"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HFO7475044.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            26010..28388
                     /gene="ppsA"
                     /locus_tag="ACHKW0_002213"
     CDS             26010..28388
                     /gene="ppsA"
                     /locus_tag="ACHKW0_002213"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HFO7475045.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGEKV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(28445..30091)
                     /gene="fadK"
                     /locus_tag="ACHKW0_002214"
     CDS             complement(28445..30091)
                     /gene="fadK"
                     /locus_tag="ACHKW0_002214"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HFO7475046.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCVRLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQLGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(30148..30441)
                     /gene="ydiT"
                     /locus_tag="ACHKW0_002215"
     CDS             complement(30148..30441)
                     /gene="ydiT"
                     /locus_tag="ACHKW0_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HFO7475047.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(30438..31727)
                     /gene="ydiS"
                     /locus_tag="ACHKW0_002216"
     CDS             complement(30438..31727)
                     /gene="ydiS"
                     /locus_tag="ACHKW0_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HFO7475048.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SGHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(31783..32721)
                     /gene="ydiR"
                     /locus_tag="ACHKW0_002217"
     CDS             complement(31783..32721)
                     /gene="ydiR"
                     /locus_tag="ACHKW0_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HFO7475049.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIIDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGAKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(32741..33505)
                     /gene="ydiQ"
                     /locus_tag="ACHKW0_002218"
     CDS             complement(32741..33505)
                     /gene="ydiQ"
                     /locus_tag="ACHKW0_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HFO7475050.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAVEKIGFDLLIFGEGSGDLYAQQAGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASGIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            33821..34267
                     /locus_tag="ACHKW0_002219"
                     /pseudo
     CDS             33821..34267
                     /locus_tag="ACHKW0_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
BASE COUNT         8700 a         8169 c         8734 g         8723 t
ORIGIN      
        1 cttaatacag agtaattatg gtgatttaaa tattaaaagc ctggctttcg actcttttaa
       61 agaaaggctt gagtctacca tggcggcact cactttcttt atatcaacgg gtcaatgtga
      121 ctgcgatgaa atagcagagt cgaacttcaa tcgtatgatt gcctatatgt ctaacattaa
      181 ctatgatgcc tcaaagccag gagcgccagc acttagtttt gatacgtatc tgcaggacaa
      241 tgttaaatat agagtgataa ttaataatct ttatggcagt gagtttcgga taagagatat
      301 taatgagaat tttgttggga tggattttac ctgtgaattc tgacccgaaa aaatgacgaa
      361 tttgatcaat ataaaaatag gttggttatt cattatttaa ggactatata ctatgaaaaa
      421 aattatatac atcctgccgg gagcattttt gctgcgattc aaaaaaaatg agagtctcct
      481 aaagtttcct tcgataatat caatgcttaa tatcaatctt ctttttaatc cactaaatct
      541 tccgggaatg ggaagtggtg tgcttgaaga tattatgagt attccagata gttcacttag
      601 aaaacgtctc gggtatgagg ttttaagctt ctcactacag gcacattcat tgagccagga
      661 atgtattgat aagttggaca tttttttcgc tgacgatctg tttaaatatg agtcagtatg
      721 catcgccgcg atggaacatt taaaaagtaa agcaacggcc cctatacaaa acggaccgtt
      781 gccagcataa gaattaacgg tgtgccagtt cggcgtcgtc ttcgctatcc agaatggctt
      841 tatcggtttg cttcagccac tggctggtca gcgtgccagc tgtcatcgag ccactaacgt
      901 ttagcgccgt acggcccatg tcgataagcg gttcaacgga gattaacagc gccaccaggg
      961 ttactggcag gcccatcgca ggcagcacaa tcagtgcggc gaaagttgca ccaccaccga
     1021 caccggcaac gcctgcggaa ctaacggtaa caataccgac cagcgtcgca atccacatcg
     1081 ggtccagcgg gttaatgcca accgtaggcg caaccatcac cgccagcatt gccggataca
     1141 aaccggcgca gccgttctga ccaatggttg caccgaaaga ggcggcgaaa ctggcgatgg
     1201 attcaggaac gcccagacga cgcgtttgtg cttccacatt cagtgggata gacgcagcac
     1261 tggaacggct ggtaaaggca aacgtcagca caggccatac cttacggaag tacttcagcg
     1321 gactcacgcc attaatgccc agcagaatgc catgcactgc aaacataatc agcagaccga
     1381 ggtaggacgc gacaacgaaa cttcccagtt tgatgatgtc ttgcaagtta gaacctgcaa
     1441 ccactttggt cattagagcc agaacgccgt aaggggtcaa ttgcatgaca aggcgaacca
     1501 gtttcatcac ccagctttgt agggtatcga tagcggctaa gacgcgttca cctttcggcg
     1561 catcatcctt cagcagtttc aatgcagcta cgccgaggaa tgcggcaaaa attaccacgc
     1621 tgataattga cgtcggattg gctccggtaa gatcggcaaa cgggtttttc gggataaagg
     1681 acaagaccag ctgcggaacg ctcagatcag agactttacc aacatagtta ctttcaatgg
     1741 cgttcagacg tgcagtttct gcaccaccct gaaccagacc ttcagccgtc aaaccaaaca
     1801 ggttggtgac cagcacaccg accagcgccg caatcagcgt ggtaaacaaa agcgtaccga
     1861 tggtcagaaa actgattttg cctaactgag atgcgttatg cagacgggca accgcgctca
     1921 gaatagaggc gaagactaac ggcataacga tcatttgcag cagttgaaca tagccgttac
     1981 caacgatgtt aaaccactgt acagaatctt taagtacctg gctgtcagaa ccataaatgg
     2041 tatgcagggc aaggccaaaa accacaccca ttaccagacc caccagcact tttttcgcca
     2101 gactccactg tttatggcgg gtctgagcca gcgcaaacag cagtacaacg aacaccacga
     2161 tgttcgcaat taatggaaag ttcatccccg ttctcctgat cttattattt ctatcactgc
     2221 gtagcagtca tagtgttggc gcaagattaa cagaactgtg agctggctct tatattcaaa
     2281 tggaattgct tattccagaa aggtgatttt tgtcttttta gtattcaacc tgatgcttaa
     2341 taagccgatg aaactggatc tgcaaggtat tgatcatttg cgatgaccaa cgaacagcgc
     2401 tgttctcggg taatgaatgg ggcatccata ccgaccagac aaataatgcc atgcagataa
     2461 aacgttccag ttggttgccc ttttggggaa ccaggatagg caagcggaaa cgccagcggc
     2521 atggccagag caggggaaca ccggcgggtg tcagcatatc ggcaagtatg tggctcaaat
     2581 aacccagcac cattccttgt agcgcatcag ccggaatgaa ccagccttcc ggaaccttaa
     2641 ggtaaaaggt tgccagcagc gcaaataccg ccagcagact gtgggtaaaa ccacgatgcc
     2701 caaaagcgcg ggcgatcggt tttgatatcc attttaatcg ctgcccaaga aacgactttg
     2761 ggtgatcgat gtccggtaac aaacacgtca ggattgcgga agggacaata tgccaccagt
     2821 caccctgtgc cagcacgggc gtcagctcgg catttttggc aaataccgca caagcaatag
     2881 aaaagagaag gtgaccttcc gccgtcatga taaaactcac taaactgtcg atacgtacag
     2941 tataggattt ttatacagtg cacggaagtg gtgacggtgt aactctttct cttaaattga
     3001 cgattaatcc gtcagttgaa tgcaaattca gataaagaac caggggcagc gagatcactg
     3061 cccctggtca ttaaccgaga aggtcttttg cggtgagttc tgtcagcgat gagagtttga
     3121 cgtttgctaa tacaaaacgt ggatcatttt gcgcttctgg cgcaggaacg acaatggaac
     3181 gcatgcgggc tgctttagag gcgatcatgc catttactga atcttccagc gctacgcagg
     3241 tcagtgggtc aacgcccagt tttgctgcgc agtcgagata tacttgcgga tgcggcttgc
     3301 tgtaaggcag tttttcagcc gaggcgaggg catcgaaact gtcgcgtaag tcaaacatgg
     3361 tcaacacttt ttccagcata tgtagtggtg acgcggaggc cagtcccacc aataaacctt
     3421 gttctttgca taacgcaacg gcttcgcgca cgcctggtaa taatggacgt gtctcttcaa
     3481 ccagtgaaat ggcacgggca ataacccgtt ctactacttc ctgacggctt ggcccattcc
     3541 acggttgccg ggcgtaccaa agatcgacca ccatatcgat gcgtaaacct aaggtgtccg
     3601 gcagctcgtt acgacgggag atatccaccc ccaggcttgc catcacatcc agttcggctc
     3661 gatcccataa aggttctgag tcgataagta atccatccat atcaaaaatt gcagcaagaa
     3721 tctgacgcgg ggttgacatt acaacctctc ctttagagca ttatgtgtaa ggtattaagc
     3781 cgtgcttatg attaagcgcc tatcatatcg cctttaaaga atgggcgaaa acagatatct
     3841 acaggtaaac taagtgaaac gacgcgtctt aatcaaggag aactcatgac gtatcaacaa
     3901 gctggacgca ttgctgtttt gaaaaggatt ttgggttggg tgatttttat acctgcattg
     3961 atctctacat tgatttcttt gctgaaattt atgaataccc ggcaggaaaa ccaggaaggc
     4021 attaatgcgg tcatgctcga ctttactcat gtcatgatcg atatgatgca ggcgaatacg
     4081 ccttttttga atctgttctg gtataactcc ccgaccccta attttaatgg tggcgtgaac
     4141 gttatgttct gggtgatttt catcctgatt tttgtcggac tggcgttgca ggattccggt
     4201 gcccggatga gtcgccaggc gcgcttcttg cgggaaggtg ttgaagatca actcattctg
     4261 gaaaaagcca aaggggaaga ggggctaacg cgtgaacaaa ttgagtcccg tattgttgtt
     4321 ccgcaccata ctattttcct gcagtttttc tcactatata tcctgccggt tatttgtatt
     4381 gctgcaggtt atgtgttctt ttctctgctt gggtttattt aatcaaaagg ccgcacggag
     4441 cggccattat cctcaaccca tatcatgctg ctaataatct atccaatgac tgctgagcaa
     4501 taaccaaatg ctgaccgcca aataatcttg cacgatttaa cagcgtgtag agttggtaaa
     4561 ccggttgacg ttcaaggaaa tcggcaggta gcggggatac tgactgatag ccgtcataga
     4621 tttgtggcgg ctgttcagta tgcagcggta acatcgccag gtcgcactct cggtcacccc
     4681 agtagcaggc agggtcgaaa atgtacgggc catccggacc cagcgcacag ttgccggacc
     4741 ataaatcgcc gtgcaacaga gaaggctgcg gttgatgcga ggccagacgc tgctgaatat
     4801 gctcgacgat ggcgtcgata ttgccgaaag cgatcccttt ctctgctgcc agttccaact
     4861 gccagccaat ccgttgttca gcaaaaaacg ttgaccagcg acgttgccag gtgttgggct
     4921 gtggagttgt ggagagcgag ttatcaaaat cgaggccaaa ttgtggttgg tcactccatt
     4981 gatgtaaacg cgcaatttgc tgaccaagaa taaatgcgct atgcgcatcc agcggacgag
     5041 gtgggagata atccatcacc agaaaactgt agtcacggtc agcgcctact gcccaaacct
     5101 taggcacggt gacggtttta ctacgcgaca gtaactccag ttggtcggct tcggcggtaa
     5161 aaccgggaag cagttccctt tcatcacatt tgacgaaaaa gtcatgtcct gcatagcgca
     5221 aatgccatgc ggcatggact tctccgccag gcagttcatt acgcagttcg atttcgcctt
     5281 cacctaactg ctcgctcaaa agacgactga ttgcctgcca catgatgtct ctcccatgtt
     5341 gtctgccaga tcataaggtt agcgcaagaa tgcggtgtaa aaacacgagc taacgcacac
     5401 cacagactga ttattgccca aaaaccattt attttctgtt gctattccat tcctcccagg
     5461 tcgtgatggt gatttcagga tctttgccgg ttgcactttg ggtaagcccc gaaaccagtt
     5521 ctctaatttc ttcttcactt tgggtactga taagtccaaa agtgttagtc cccagctcat
     5581 ggatatttcc ctcatcatcc gtcatggtga gcaaaaaacc gtcgcgagtc aggtaattat
     5641 tcaactcgtt aatttcagtc aatgtatcct catgcaacat gacggttatg acataacgga
     5701 caagatcgcc actggccata gttcacccct ttgttatcac atgacttttg agcatagtcg
     5761 gagaaacgcg ttgccgacag gttggtggtg attcccccaa tgctggggga atgtttttgt
     5821 tagcgggaaa ggtaagcgta aattttttgc gtatcgtcat gggagcacag acgtgttccc
     5881 tgattgagtg tggctgcact ccccgcagct acgccaaaac ggaccatctc ttcaagagag
     5941 gcattttctg ccagtttcag tgtcatcgcg ccgaccatgc tgtcaccagc gccaacggta
     6001 ctctggcttt tcaccggtgg tggcaccacc tgaatgcagt tttcactatc aacacccagc
     6061 gctccttgtg gaccaaggga aacgacaacc cgtttggcct tgccgctatt aacgatttcc
     6121 tgcgcggctt tgcggacatc gtctggctgg gtgagttcgc gattcaccag cgcactgagt
     6181 tctttttggt taggcttaac caactcgatg ttaccaatcg ccagtgctgc acttaacgct
     6241 tcgccagaac tgtcgatgat gcagcggatc ccttggtttt gcgcagcgga aatcagttgg
     6301 gttaattttt ccagcttcac acctggcggc aggcttccgc ttatgaccag gatggccccg
     6361 gattcaattt ccagaacttg ctcttcaagc tggcgaaact catcttcatt taatgccgcg
     6421 cctggcataa caaaacgata ctgctcaccg cttgcttcca catgtacgtg taaattctgc
     6481 cgggtccagt ctttggcttc tacagtagcg acggggacat tttcatccgc caacagagaa
     6541 accaggtgtt cgccggtcgc gccacccgcc gggaagatcg ctgtggcact gcctccaaga
     6601 tgggcaatgg cgcgggcgac gttgatgccg ccgcccccgg gttcgaacac cggtgcggta
     6661 cagcgcagtt ttccttcggg ataaatttgc ggggtaattg ttgcgctatc gagagagggc
     6721 gcaagtgtca acgtatagat acgtaccatc atttcctcct ataggctgat ttcagtctgg
     6781 caccgaatca gccgaaagtg aagaaattaa caatatgatt tattagagct ttatttaaaa
     6841 ttttgcagat aaatatatat aaataaaaat ctgttttcgc cacatgctta ttcaaaaaat
     6901 gaaataagaa tacattgcta agttattcgc gcctttttat aattcgttac ctttccaggg
     6961 acgctaatct ttgatcctta agaaagtttc cgggaccgcg atggtctctt tttttgttgt
     7021 acggactctt gataaatgaa gcttttgaag acagttcctg ccatcgttat gttggcgagg
     7081 ggcatgtttg cgtcactgaa tgcagctgcc gatgattccg tttttactgt catggacgat
     7141 cctgccagcg ctaaaaaacc tttcgaaggt aatctgaacg caggatatct tgcacaatca
     7201 ggcaatacga aaagctcctc acttacggct gacaccacca tgacctggta tggacaaacc
     7261 actgcctggt cgctgtgggg gaacgccagt aatacttctt ctaatgatga gcgttcttca
     7321 gaaaaatatg cggcgggcgg gcgtagccgt tttaatttaa ctgattatga ctatttattt
     7381 ggtcaggcaa gctggctgac agaccgttat aacggctatc gagagcgcga tgtgttgacc
     7441 gcgggttatg gtcgtcagtt tttgaatggc ccggtacaca gtttccgctt tgaattcggt
     7501 ccaggtgtgc gttacgacaa atataccgac aatgccagtg aaactcagcc gctgggctat
     7561 gcctccggtg cttatgcatg gcagttgact gacaacgcta aatttacgca gggtgtttcc
     7621 gtctttggcg cggaagacac aacgctgaac tccgaaagcg cattaaatgt tgctattaat
     7681 gaacattttg gactgaaagt ggcttacaac gtcacatgga actctgaacc tccagagtct
     7741 gcaccggaac ataccgatcg tcggataacc ttatcgctgg gctacagcat gtaaaattat
     7801 ctgccggacc gtgttatgcg gtctggctgt cttcgtttgt cactgctcag ccccaaatca
     7861 ttgctgccca acgtagcaac aacatagcgc cacagattgc cagtagcacc accaccatct
     7921 tccagtgttt tttatcaaag cgtttagtcg gcataccagt gtcctgcata tctcttgtgc
     7981 gtgataacgg agtgtaacaa aagggacagg catcaaggga ttttttgtta ttacagccgt
     8041 cgggtggcgt tgtacacctg atcactgttt ctgcgtttta tgaaaaaaat aaaattatgt
     8101 atcgatatac aaatatcata aatagctggt tataaatacg tttttagttc atcagcacta
     8161 aaactcaggc tttggctgat tgcattaata gcagcataat atgtttctac agttttataa
     8221 tgattatgat gaatggctat atgaacagct gacatgtttt catgattttt agcggccaac
     8281 agtgtgaata attgacgttg actaaaagaa tatttttttg caaaagtact cagtgttgat
     8341 atataagata acacgacgtc tttattaccc ttgctcatgg cgatgtataa agcaggggtt
     8401 ccatgtgctg tcgcgccttt taataagtcc atgatgttga ttttgctgag tttatattta
     8461 acggcgatcc cataaacttt agagaggaac cgtgtggcac acaaagggtg attattttcc
     8521 attgcagcaa ataaccctgg ttcaccatca cgtgttttca tggctaaaat atccatcacc
     8581 atttttttgt ttaaaagttc ttgtgctatt ggagtggtta atgcattcag aaagatatcg
     8641 attatttcat cgaatccata caacatggag atatagagtc caggtgtttc gttagcactt
     8701 ttagtttgca gtaatttaac cagattatct tcatgaatga tatgattgta taccagtgac
     8761 tggatttctt gcataaatat ttttacgttg tcagtttgtc cgttcatcat agctagaaat
     8821 aaagcaggtg taccaacgct attctgcgca gttattattt caatgatttc tttttcaggg
     8881 agtatgggca actgtttgaa tagtgtaata agctattgag tctggcctgt ggccattatt
     8941 aacaacattg ccgatggatg tataggggca aatggcattc gtgtcgtgat acccgctaaa
     9001 atagcaactg gaactccctc aggtaataga ggaagaatag caattgcaca gttgcgaaca
     9061 tacctatcag cccaggtctg agcatcggga gctgaactga tgaacttatc aagcgagaga
     9121 aagcgccgtg ctgtcctgtg ggtgcctctg acggcaacgt tggtatcgtt cgggtcgtaa
     9181 acagacacaa cataatttaa ttgtccatac actgactgct ttattcgtag ccttactgac
     9241 aaagtatgat tgtctatata taaaatcgcg gacatatgac tgacattgtt tgccatcatt
     9301 cgacaaaaga aaatatctaa agcgaagcca aaatccttac tcgcgataac gcgactccct
     9361 gcaggaggat ggcgaagtaa tgctctgaac tccgcatccc ggactaaggg gatatgctgc
     9421 tgtatggatt caacggagga gtaatgttta atcaggttga ctttgccttt tccatttctc
     9481 aatgaatcaa gggcgtattg caatgacaga tggcgacaaa aaatagcgtc agaaggagat
     9541 tgcaaaaaac atgcactacc attgagattt actgttgggt gtatagtttt ttctctatgg
     9601 atttttttag acagtttcat acaatggtta cgatcttcag cagttaactg ttcctgttca
     9661 atataatcca gtatgcttcg caaatggtta gctttaatag gcgtggtttc cagtaataag
     9721 gaaatgaaat cttttttata gtgtaatgta atgagtatat tttctgcatg gatgggtgaa
     9781 gtggcaaagt taatggtaaa aagggcagtg gaataacaat tttgtgatat ttttcgacag
     9841 tttgcggtaa attcctgaat aacacagtat gacagcatcg aacttaagtc agaggtatca
     9901 agtagggtga tgagctggcg cgcaaattta ttaataggct ctgatgaagg tgttggtatg
     9961 tcgctatcga tgtgcaatct atgaggagac ttataatgag ttctgataat gatatcgttt
    10021 tgactcataa tctattcgcc ttatatatat ctggaggttt tgaggatgat gattaattat
    10081 tatcgccaga acgaaatgtg cacgaaaaat tgttataaga attgtgtcac ttttatcatt
    10141 gtgatatgac cacttcgtca ttaatctcat gagcgtttaa aatactaccg ccaaatcacg
    10201 caacgaactt tgctggattc agtttatgct gctgtaaatc cgctcgcagt aaacctttca
    10261 gacgcaccgt gatgttatca gtggttcttt aagcgttttg ctggtgtact cactacaaac
    10321 gaattgcgaa tcgaatcaat gtgaaacgga aaggtacaat ctcccttttt ttagtgttaa
    10381 catcgctcaa ccgggtggaa ggtgagaggt taatgtcttt catttgtgta cctgattgcg
    10441 aaccaattta gcatttgttg gctagatggt ttcgcaatga actgttaata aacaaatttt
    10501 tctttgtatg tgatctttcg tgtgggtcac cactgcaaat aaggatataa aatgcctgtt
    10561 ataactcttc ctgatggcag ccaacgccat tacgaccacg ctgtaagccc catggatgtt
    10621 gcgctggaca ttggtccagg tctggcgaaa gcctgtatcg cagggcgcgt taatggcgaa
    10681 ctggttgatg cttgcgatct gattgaaaac gacgcacaac tgtccatcat taccgccaaa
    10741 gacgaagacg gtctggagat cattcgtcac tcctgtgcgc acctgttagg gcacgcgatt
    10801 aaacaacttt ggccgcatac caaaatggca atcggcccgg ttattgacaa cggtttttat
    10861 tacgacgttg atcttgaccg cacgttaacc caggaagatg tcgaagcact cgagaagcgg
    10921 atgcatgagc ttgctgagaa aaactacgac gtcattaaga agaaagtcag ctggcacgaa
    10981 gcgcgtgaaa ctttcgccaa ccgtggggag agctacaaag tctccattct tgacgaaaac
    11041 atcgcccatg atgacaagcc aggtctgtac ttccatgaag aatatgtcga tatgtgccgc
    11101 ggtccgcacg taccgaacat gcgtttctgc catcatttca aactaatgaa aacggcaggg
    11161 gcttactggc gtggcgacag caacaacaaa atgttgcaac gtatttacgg tacggcgtgg
    11221 gcagacaaaa aagcgcttaa cgcttacctg cagcgcctgg aagaagccgc gaaacgcgac
    11281 caccgtaaaa tcggtaaaca gctcgacctg taccatatgc aggaagaagc gccgggtatg
    11341 gtattctggc acaacgacgg ctggaccatc ttccgtgaac tagaaatgtt tgttcgttct
    11401 aaactgaaag agtaccagta tcaggaagtt aaaggtccgt tcatgatgga ccgtgtcctg
    11461 tgggaaaaaa ccggtcactg ggacaactac aaagatgcaa tgttcaccac gtcttctgag
    11521 aaccgtgaat actgcattaa gccgatgaac tgcccgggtc acgtacaaat tttcaaccag
    11581 gggctgaagt cttatcgcga tctgccgctg cgtatggccg agtttggtag ctgccaccgt
    11641 aatgagccgt caggttcgct gcatggcctg atgcgcgtgc gtggatttac ccaggatgac
    11701 gcgcatatct tctgtactga agaacaaatt cgcgatgaag ttaacggatg tatccgttta
    11761 gtctatgata tgtacagcac ttttggcttc gagaagatcg tcgtcaaact ctccactcgt
    11821 cctgaaaaac gtattggcag tgatgaaatg tgggatcgtg ctgaggcgga cctggcggtt
    11881 gcgctggaag aaaacaacat cccgtttgaa tatcaactgg gtgaaggcgc tttctacggt
    11941 ccgaaaattg aatttaccct gtatgactgc ctcgatcgtg catggcagtg cggtacagta
    12001 cagctggact tctctttgcc ttctcgtctg agcgcttctt atgtaggcga agacaatgaa
    12061 cgtaaagtac cggtaatgat tcaccgcgca attctgggat cgatggaacg tttcatcggt
    12121 atcctgaccg aagaattcgc tggtttcttc ccgacctggc ttgcgccagt tcaggttgtt
    12181 atcatgaata ttaccgattc acagtctgaa tacgttaacg aattgacgca aaaactatca
    12241 aatgcgggca ttcgtgttaa agcagacttg agaaatgaga agattggctt taaaatccgc
    12301 gagcacactt tgcgtcgcgt cccatatatg ctggtctgtg gtgataaaga ggtggaatca
    12361 ggcaaagttg ccgttcgcac ccgccgtggt aaagacctgg gaagcatgga cgtaaatgaa
    12421 gtgatcgaga agctgcaaca agagattcgc agccgcagtc ttaaacaatt ggaggaataa
    12481 ggtattaaag gcggaaaacg agttcaaacg gcgcgcccta accgtatcaa tggcgaaatt
    12541 cgcgcccagg aagttcgctt aacaggtctg gaaggcgagc agcttggtat tgtgagtctg
    12601 agagaagctc tggagaaagc agaagaagcc ggagtagact tagtcgagat cagccctaac
    12661 gccgagccgc cggtttgtcg tataatggat tacggcaaat tcctctatga aaagagcaag
    12721 tcttctaagg aacagaagaa aaagcaaaaa gttatccagg ttaaggaaat taaattccgt
    12781 cctggtacag atgaaggcga ctatcaggta aaactccgca gcctgattcg ctttctcgaa
    12841 gagggtgata aagccaaaat cacgctgcgt ttccgcggtc gtgagatggc gcaccagcaa
    12901 atcggtatgg aagtgcttaa tcgcgtgaaa gacgatttgc aagaactggc agtggtcgaa
    12961 tccttcccaa cgaagatcga aggccgccag atgatcatgg tgctcgctcc taagaagaaa
    13021 cagtaaggcc ttcaagtagc actgtctgtg gagccttcgg gcttcacagg ctttgttcgc
    13081 ctttgtttcg tttattaaca atgcgaagtg gaagttatta aaatgccaaa aattaagacc
    13141 gtacgcggtg ctgctaagcg cttcaaaaaa accggtaaag gtggttttaa gcacaagcac
    13201 gctaacctgc gtcacattct gaccaaaaaa gcgaccaaac gtaaacgtca cctgcgtccg
    13261 aaagccatgg tttccaaagg cgatctgggc ctggtaatcg cgtgcctgcc gtacgcataa
    13321 gccgttaacg tttttaactt tttaattaga atatagatac aggagagcac atatggctcg
    13381 cgtaaaacgt ggtgttattg cacgcgcacg tcacaagaaa attttgaaac aagctaaagg
    13441 ctactacggt gcgcgttctc gcgtataccg cgttgccttc caggctgtta tcaaagctgg
    13501 tcagtatgct taccgtgacc gtcgtcaacg taagcgtcag ttccgtcaac tgtggattgc
    13561 gcgtatcaac gcagcagcac gtcagaacgg tatttcttac agcaaattca tcaatggcct
    13621 gaaaaaagcc tctgttgaaa tcgaccgtaa gatcctggct gatatcgcag tattcgacaa
    13681 agtagcgttc accgctctgg ttgaaaaagc gaaagcagct ctggcataag ccagttgaaa
    13741 gagggagcta gtctccctct tttcgtttca acgccatcaa aacattgact tttatcgccg
    13801 tagccttttc aataaaggtc ttttgaagag taccaaaagg taacgcaagc aatgaatgct
    13861 gctattttcc gcttcttttt ttactttagc acctgaatcc aggaggctag cgcgtgagaa
    13921 gagaaacgga aaacagcgcc tgaaagcctc ccagtggagg ctttttttgt atgcgcgttt
    13981 tgaaaaataa aaagcctgtc tcatcaggtg taattgtcgc agccagtctg gacacggcca
    14041 gcgcggagaa accggagcgt atttaagtac gtgagaattt cgagcactgc ccgggaccaa
    14101 aatggcaagt aaaatagcct gatgggatag gctctaagtc caacgaacca gtgtcaccac
    14161 tgacacaatg aggaaaacca tgtcacatct cgcagaactg gttgccagtg cgaaggcggc
    14221 cattagccag gcgtcagatg ttgccgcgtt agataatgtg cgcgtcgaat atttgggtaa
    14281 aaaagggcac ttaacccttc agatgacgac cctgcgtgag ctgccgccag aagagcgtcc
    14341 ggcagctggt gcggttatca acgaagcgaa agagcaggtt cagcaggcgc tgaatgcgcg
    14401 taaagcggaa ctggaaagcg ctgcactgaa tgcgcgtctg gcggcggaaa cgattgatgt
    14461 ctctctgcca ggtcgtcgca ttgaaaacgg cggtctgcat ccggttaccc gtactatcga
    14521 ccgtatcgaa agtttcttcg gtgagcttgg ctttaccgtg gcaaccgggc cggaaatcga
    14581 agacgattat cataacttcg atgctctgaa cattcctggt caccacccgg cgcgcgctga
    14641 ccacgacact ttctggtttg acactacccg cctgctgcgt acccagacct ctggcgtaca
    14701 gatccgcacc atgaaagccc agcagccacc gattcgtatc attgcgcctg gccgcgttta
    14761 tcgtaacgac tacgaccaga ctcacacgcc gatgttccat cagatggaag gtctgattgt
    14821 tgataccaac atcagcttta ccaacctgaa aggcactctg cacgatttcc tgcgtaactt
    14881 ctttgaggaa gatttgcaga ttcgcttccg tccttcctac ttcccgttca ccgagccttc
    14941 tgcagaagtt gacgtcatgg gtaaaaacgg taaatggctg gaagtgctgg gttgcgggat
    15001 ggtgcatccg aacgtgctgc gtaacgttgg catcgacccg gaagtttact ctggtttcgc
    15061 cttcgggatg gggatggagc gtctgactat gttgcgttac ggcgtcaccg acctgcgttc
    15121 attcttcgaa aatgatctgc gtttcctcaa acagtttaaa taaggcagga atagattatg
    15181 aaattcagtg aactgtggtt acgcgaatgg gtgaacccgg cgattgatag cgatgcgctg
    15241 gcgaatcaaa tcactatggc gggcctggaa gttgacggtg tagaaccggt tgccggtagc
    15301 ttccacggcg tggtcgttgg tgaagtggtt gagtgtgcgc agcatccgaa cgctgacaaa
    15361 ctgcgtgtga caaaagtgaa tgttggcggc gatcgcctgc tggacatcgt ctgcggtgcg
    15421 ccaaactgcc gtcagggcct gcgtgtggcg gtagctacca ttggtgctgt tctgccgggt
    15481 gatttcaaaa ttaaagcggc gaaactgcgt ggtgaaccgt ctgaagggat gctgtgctcc
    15541 ttttctgagc tgggaatttc tgacgatcat agcggcatta tcgagctgcc tgcggatgcg
    15601 ccgattggca ccgacatccg tgaatacctg aaacttgatg acaacaccat cgaaatcagc
    15661 gtgacgccaa accgtgccga ctgtttaggt atcattggtg ttgcgcgtga cgttgccgtg
    15721 ctgaatcagc tgccgctggt tgaaccggaa atcgttccgg ttggtgcgac catcgacgac
    15781 acgctgccga ttacagtcga agcgccggaa gcctgcccgc gttatcttgg ccgtgtggta
    15841 aaaggcatta acgttaaagc gccaactccg ctgtggatga aagaaaaact gcgtcgttgc
    15901 gggatccgtt ctatcgatgc agttgttgac gtcaccaact atgtactgct cgaactgggc
    15961 cagccgatgc acgctttcga taaagatcgc attgaaggcg gcattgtggt gcggatggcg
    16021 aaagagggcg aaacgttggt actgcttgat ggcactgaag cgaagctgaa tgctgacact
    16081 ctggtcatcg ccgaccacaa caaggcgctg gcgatgggcg gcatctttgg tggcgaacac
    16141 tctggcgtga atgacgaaac gcaaaacgtc ttgctggaat gcgctttctt cagcccgctg
    16201 tctatcaccg gtcgtgctcg tcgtcatagc ctgcatactg atgcgtctca ccgttatgag
    16261 cgtggcgttg atccggcact gcagcacaaa gcgatggaac gtgcgacccg tctgctgatc
    16321 gacatctgcg gtggtgaggc tggtccggta attgatatca ccaacgaagc aacgctgccg
    16381 aagcgtgcaa ccatcacttt acgtcgtagc aaactggatc gcctgatcgg ccatcatatt
    16441 gcggatgagc aggtaactga cattctgcgt cgtctcggct gcgaagtgac cgaaggcaaa
    16501 gacgagtggc aggcagttgc gccgagctgg cgtttcgata tggagattga agaagatctg
    16561 gtcgaagaag tcgcgcgtgt ttacggctac aacaacatcc cggatgagcc ggtacaggca
    16621 agcctgatta tgggtactca ccgtgaagct gacctgtcgc tcaagcgcgt gaaaacgctg
    16681 ctcaacgaca aaggctatca ggaagtgatc acctacagct tcgttgatcc gaaagtgcag
    16741 cagatgatcc atccaggcgt tgaagcctta ctgctgccaa gcccgatctc tgttgaaatg
    16801 tcagcaatgc gtctttctct gtggaccggc ctgctggcaa ccgtggtgta caaccagaac
    16861 cgtcagcaga accgtgtgcg cattttcgaa agcggtctgc gtttcgtacc agatactcag
    16921 gcaccgttgg gcattcgtca ggatctgatg ttagccggtg tgatttgcgg taaccgttac
    16981 gaagagcact ggaacctggc aaaagagacc gttgatttct atgatttgaa aggcgatctt
    17041 gaatccgttc tcgacctgac cggtaaactg aatgaggttg agttccgtgc agaagcgaat
    17101 ccggcactgc atccggggca atccgcagcg atttatctga aaggtaaacg tattggtttt
    17161 gttggggttg ttcatcctga actggaacgt aaactggatc ttaacggtcg cactctggtg
    17221 ttcgaactgg agtggaacaa gctcgcagac cgcgtggtgc ctcaggcgcg cgagatttct
    17281 cgcttcccgg cgaaccgtcg tgacatcgcg gtggtggtcg cagaaaacgt tcccgcagcg
    17341 gatattttat ccgaatgtaa gaaagttggc gtaaatcagg tagttggcgt aaacttattt
    17401 gacgtgtacc gcggtaaggg tgttgcggag gggtataaga gcctcgccat aagcctgatc
    17461 ctgcaagata ccagccgtac actcgaagaa gaggagattg ccgctaccgt cgccaaatgt
    17521 gtagaggcat taaaagagcg attccaggca tcattgaggg attgaaccta tggcgcttac
    17581 aaaagctgaa atgtcagaat atctgtttga taagcttggg cttagcaagc gggatgccaa
    17641 agaactggtt gaactgtttt tcgaagagat ccgtcgcgct ctggaaaacg gcgaacaggt
    17701 gaaactctct ggttttggta acttcgatct gcgtgataag aatcaacgcc cgggacgtaa
    17761 cccgaaaacg ggcgaggata ttcccattac agcacggcgc gtggtgacct tcagacccgg
    17821 gcagaagtta aaaagccggg tcgaaaacgc ttcgcccaaa gacgagtaat ctgatctaac
    17881 taaaaaggcc gctctgcggc cttttttctt ttcactgtcg aagagtcacc gtaaaatcaa
    17941 cgccatgaca cttcagcaga acggatacca tgctgacact tgcccgccaa caacagcgac
    18001 aaaatattcg ctggttatta tgcctgtcag ttttgatgct gctggcgctt ctcttaagcc
    18061 tttgcgccgg tgaacaatgg atctcgccag gtgactggtt tactcctcgt ggcgaactgt
    18121 tcgtctggca aattcgcctg ccacgtacgc tggctgtatt gctggttggt gcggcgctgg
    18181 ctatatccgg cgctgtaatg caggcgttgt ttgaaaatcc tctggcagaa cctggactac
    18241 ttggcgtctc taacggcgca ggcgtggggc ttatcgccgc ggtattgctt gggcaagggc
    18301 aactccccaa ctgggcgcta gggctgtgtg cgattgctgg cgcgcttatc atcactttaa
    18361 tactcttacg tttcgcccgt cgtcatcttt cgaccagtcg gttattgctg gctggcgttg
    18421 cattagggat tatctgtagc gcactaatga cgtgggctat ctacttttcc acctctgttg
    18481 atttacgtca gctgatgtac tggatgatgg gcggttttgg cggcgtagac tggcggcaaa
    18541 gctggctgat gctggcattg atccccgtgt tgttgtggat ctgttgtcag tccaggccga
    18601 tgaatatgtt agcacttggc gagatctcgg cgcggcaact gggtttaccc ctgtggttct
    18661 ggcgcaatgt gctggtggcc gcgaccggct ggatggttgg cgtcagtgtg gcgctggcgg
    18721 gtgctatcgg ctttattggt ctggtgatcc cacatattct tcggttgtgt ggtttaaccg
    18781 atcatcgcgt attacttccc ggctgcgcgc tggcaggggc gagcgcattg ctgctggccg
    18841 atattgtagc gcgcctggca ttagctgccg cagagctgcc tattggcgtg gtcaccgcaa
    18901 cgttgggtgc gccggtgttt atctggttat tgttaaaagc aagacgttag ccgcaaaaag
    18961 acggtctatg attaaaagct aagattttta ctgaccacac ccaggagaaa cgatgcaaga
    19021 ttccattctg acgaccgtag tgaaagatat cgacggtgaa gtgaccacgc tggagaagta
    19081 cgctggtaat gtgctgttga ttgtcaatgt cgcctcaaag tgtggcttaa cgccgcaata
    19141 tgagcagttg gagaatattc agaaagcctg ggccgatcga ggttttgtgg tgctgggatt
    19201 cccgtgcaac cagtttctgg aacaagaacc gggcagcgat gaagagatta aaacttactg
    19261 taccaccaca tggggggtga cgttcccgat gttcagtaag attgaagtta atggcgaagg
    19321 acgccatccg ctgtatcaaa aattgattgc cgcagcgccg accgcagtcg cgccggaaga
    19381 gagcggattc tatgcccgta tggtcagcaa aggtcgtgca ccgctgtacc ctgatgatat
    19441 tttatggaat tttgagaaat ttttagttgg cagggacggg ttggtcatcc agcgtttttc
    19501 cccggatatg acgcctgaag atcccattgt gatggaaagc attaaactgg cgttggcaaa
    19561 ataatgtcta ttgtgatgca gttacaggat gttgcggaaa ctacccgcct ggggccgctt
    19621 tctggcgagg ttcgggctgg ggagatcctg cacctggtgg ggccgaatgg cgcgggtaag
    19681 agtaccttgc ttgcacgaat ggccggaatg accagcggta agggaagcat tcagttcgcg
    19741 gggcaaccac tggaagcatg gtccgcaaca aaactcgcgc tgcatcgcgc ctacctttca
    19801 caacagcaga cgccgccgtt tgcaatgccg gtctggcact acctgacact gcatcagcac
    19861 gataaaacgc gtaccgaact actgaatgat gtcgcagggg cgctggctct tgatgacaaa
    19921 ctcggacgta gcaccaatca actttccggc ggtgaatggc aacgcgtacg tcttgctgcg
    19981 gtggtgctgc aaatcacacc acaagccaat cccgcaggtc aattgctgct tcttgatgag
    20041 ccgatgaaca gtcttgatgt tgcgcaacaa agtgcgttag acaaaattct gagcgctctg
    20101 tgtcagcaag gactggcgat tgtgatgagc agtcacgatc tcaaccacac attgcgtcat
    20161 gcgcatcggg cgtggttgct aaaaggtgga aaaatgctgg ccagtggacg cagagaagag
    20221 gtgctcacgc cgccaaatct ggcgcaggcc tatgggatga attttcgccg tctggatatc
    20281 gaaggtcaca gaatgctgat ttcgaccatc tgatttcatt agtatcttga aaatcgggta
    20341 attacgacgc taaattaatg caagaataaa aaacagagga accgtacgga atgcgtttct
    20401 gccttatttt gatcacagca ctgtttctgg ccgggtgtag ccaccataaa gcaccgccgc
    20461 caaatgccag actttctgat tcgattaccg ttattgccgg tttgaacgac cagctacaaa
    20521 gctggcatgg cacgccgtat cgttatggtg gcatgacgcg gcgcggtgtg gactgttcgg
    20581 gatttgtggt tgtaacgatg cgcgatcgtt tcgatttgca gctgccccga gaaaccaaag
    20641 aacaagcctc tatcggcacg caaattgata aagacgagtt gctgcctggt gacctggtct
    20701 ttttcaaaac gggttccgga caaaatggtt tgcatgtagg tatttacgat accaacaacc
    20761 aatttatcca cgcctctacc agcaagggag tgatgcgttc ctcacttgat aatgtctatt
    20821 ggcagaaaaa tttctggcag gcgagacgaa tttagtatgt cgaaaaatgg acggcgatga
    20881 tacatcgccg tccatttact cctgaataca gtagtatttt gcagacaaaa attccgtaaa
    20941 tggattattc gtttataatc aggataatct aaaaaaagga cccctgaaaa agtggttaac
    21001 atggaacaaa tgaaagttac tccttctgta attgttatgt cttatggcct catccctttt
    21061 agccggatac tgaaaaacat ccttcgagag ggacggttac catgaagatt tttttggaga
    21121 atctttatca ttctgattgc tatttcctgc cgataagaga taaccagcaa ctccttgtcg
    21181 gcgttgagtt aattacccat ttctccagtg aagatggcac ggtgcgtatc ccgacgagtc
    21241 gggttatagc gcaacttacc gaggagcaac attggcaatt attttcagag cagttggaat
    21301 tactaaaatc atgccaacat ttttttattc aacataaact ctttgcctgg cttaatttaa
    21361 cgccacaagt ggctacatta ttattagaga gggataatta cgcaggcgaa ctattaaaat
    21421 atccgtttat tgaactgttg attaatgaga attacccgca tctcaatgaa ggaaaagata
    21481 atcgcggtct tttatcctta tcgcaagtgt atccccttgt gttaggtaat ttgggagcag
    21541 gcaatagcac aatgaaagcg gtttttgatg gtcttttcac ccgcgtcatg ctggataaaa
    21601 gttttattca gcaacaaatc acgcaccgct ccttcgaacc gtttattcgt gcgatccagt
    21661 cgcaaatttc cccttgttgc aattgcatca ttgcgggagg cattgatacg gcagaaatat
    21721 tggcacagat tacccccttc gatttccatg ctctccaggg atgcctgtgg cctgccgttc
    21781 ccataaatca gataacgacg ttggttcagc gataaccctt ctgtttgctg gtgtttaaga
    21841 cgagagtaac cgtctacact atcaaacagg aggatctatg accctgtctt ttattacccg
    21901 ctggcgcgat gaattgccag aaacctatac agcactttcc cctacgcctt taaataatgc
    21961 ccggctgatt tggcataata ccgaactcgc taacacgctg agtattccat cgtcgctgtt
    22021 taaaaatgct gcaggtgtct ggggcggcga aaccctactg cctggtatgt caccactggc
    22081 gcaggtttac agtggtcatc agttcggcgt ctgggcgggc caactgggtg atgggcgcgg
    22141 cattttactt ggcgaacaac tgcttgctga tggcactaca atggactggc atctgaaagg
    22201 tgctggcctg acgccttatt cgcgaatggg tgatggacgg gcggttttac gttcgacgat
    22261 acgagaaagt ctcgccagtg aggcgatgca ttatctgggc attccgacga cccgcgcgtt
    22321 aagtatcgtc accagcgatt cgccagtgta tcgggaaacg gtggagccag gcgcgatgct
    22381 gatacgtgtg gcaccaagtc atctgcgctt tggtcatttc gaacattttt actatcgccg
    22441 cgagccggaa aaggttcgtc agttagctga ctttgccatt cgtcattact ggtcacatct
    22501 tgaagatgat gaggacaaat accgtctctg gtttaacgat gttgtcgcac gtaccgcatc
    22561 gttaattgcc caatggcaga cggtcggctt tgctcatggg gtgatgaata ccgacaacat
    22621 gtcgctgctg gggctgacgc ttgattacgg gccgtttggt tttcttgatg attacgaacc
    22681 cggttttatt tgtaatcact cggatcatca agggcgttac agctttgata atcaacctgc
    22741 cgtcgcgttg tggaatttac agcgtctggc gcagacattg tcaccatttg ttgccgtaga
    22801 tgccctgaat gaggccctgg acagctatca gcaggttttg ttgacgcatt atggacaacg
    22861 gatgcggcag aaactgggct tcatgacgga gcaaaaagag gataacgcgc tactgaatga
    22921 attattcagt ctgatggcgc gagagcgcag cgattatacc cgcacattcc gcatgctgag
    22981 tctgaccgag cagcacagcg cggcgtcacc gctacgtgat gagtttattg atcgtgcggc
    23041 atttgatgac tggtttgccc gttatcgggg gcgtttgcaa caagacgagg ttagcgatag
    23101 tgagcgtcag cagctgatgc aaagcgttaa cccagctctg gtgttgcgca actggttggc
    23161 gcaacgggcg attgaagcgg cagaaaaggg tgatatgacg gaattgcacc gcctgcatga
    23221 ggcgttgcga aatcctttca gcgacagagc tgatgactat gtcagtcgtc cacctgactg
    23281 gggtaaacgg ctggaagtca gctgctcgag ttaaaggcta cttggtcaac agcagcttgc
    23341 cagcctgagt tttacggagc agatattctt gcccgtcatg atcgataatc agtttgccat
    23401 ccggacctaa cagtgtctgg ctggaaattc gccgaatagg ctgcggggaa gcggtcttgt
    23461 gattggcatc cgtttcaggc gtgagttttc tgctatccgt ataacgcata aaaattaata
    23521 ataattgata taacaatgat tcttattatc aatattatgg ctgaccagag caagcagtaa
    23581 tgtgctgaga agacaacaag ttgcgcagaa atgcccatcc catcaggaat gggcacgcat
    23641 tcagaagcgg gtatctaccg cagaggcaag tttttcgacc aggcgttcgg tatcttccca
    23701 gcccagacac gggtcggtaa tggattgacc gtaagtgagc ggctgaccgc cgacgatttt
    23761 ttgcgttcct tcgcgcagga aactttccgc cataattcca gcaatcgccg tagagccatt
    23821 gcggatttgc tgacaaatat cctcacaaac ttctaactgg cgacggtgct gcttctggca
    23881 gttaccgtgg ctgaaatcca ccaccagatg ttcaggtaaa tcaaactcgt gcagcgtatc
    23941 gcaggctgcg gcgatatcat cggcatgata attcggtttt ttgccgccac gcataataat
    24001 gtggccatac gggttgccgc tggtctgata gatggtcatc tgaccatttt tgtctggcga
    24061 gagaaacata tggctggcgc gggctgcgcg gatagcatcc acggcaatcc gcgtgttgcc
    24121 atcggtacca tttttaaaac ctaccggaca ggagagtgcc gaagccattt cacggtggat
    24181 ctgactttcg gtagtacgtg cgccaatcgc gccccaactg attaaatcag caataaactg
    24241 accggtcacc atatcgagga actcagtcgc ggtagggaca cccagctcat ttacctgtaa
    24301 aagtaatttg cgcgccagct ccagaccgtg atttacccga tagctgccgt ttaaatccgg
    24361 atcggagatt agccctttcc agccgacaac agttcgtggt ttttcaaaat aggtgcgcat
    24421 tacgatttcc agccgtgact ggtactggtt gcgcagcgac tgcagacggg tggcgtactc
    24481 cattgcagcg gtgagatcgt ggatcgagca ggggccaata atgaccaaca gtcgcttatc
    24541 ttcaccattc agtatttttt caattctgcg gcgggagtcg gtgacatggg tggcgacgcc
    24601 aggcgttacg ggataccgta gcgcgagttc ggcgggcgtt accaggctct caatacgcgc
    24661 agtacggagt tcgtcagttc tgttcattac aagtctcaga aatttgttgc ttctacggca
    24721 ggactaccgg gaagtgatgc gcatcacgat aaaccaatcg cagctgattt caagtgcgag
    24781 gtgtgtcaca cctcgccggg tggttagcat gataacaaaa aaataagcta atgcactagt
    24841 tctctagtac attcggcggc taagccccat gatatcgagg atcttggtgg caatctcttc
    24901 taccgaataa ttggtactgt taatccacgg gatctgattt ttacggtaca acgcttctac
    24961 ttccgcgact tccatcctgc actgacgaag cgaggcatag cggctgttct cccgacgttc
    25021 ctcgcgaatc gccgccagac gttccgggtc gatagtcagg ccgaacaatt tatgctgaag
    25081 cggtttgagc gacgcgggta gcaccagatt gtccatatcg tcggcaataa aggggtagtt
    25141 tgccgcgcgg ataccaaact gcattgccag atacagactg gtgggggttt taccacagcg
    25201 agaaacaccg agcaggatca cctgagcctg gtccagattg cgcaacgaaa tgccgtcatc
    25261 gtgggcgagg gtgtaatcaa tcgccgcaat gcgcgcatca tatttattga gattattagg
    25321 gttaaggcca tgggtacgat gagcaatcgg cgttggatcc agtttcatct cttgttgtag
    25381 cggggcaacc agcgcctgaa caatatcctg gcaaaagcct tcactttgca agatgatggc
    25441 gcgaatctcc ggcaacacga tggagtagaa gaccagcggg cgcacacctg tctggtgata
    25501 aattgcgtca atctgatcct tcactgcccg tgcacggctc tcattttcga caaacggcag
    25561 cgtaatgctg ctgatagtga cgggaaattg tgacattact gcgtgtccta atacctccgc
    25621 agttattgcc gtaccatcag aaatataaaa aacgtggcga tcaacagcat tatccatttt
    25681 gttcttcccg tgatgcagac ataattctat gaaagcataa attaaaaacg cacagaagcg
    25741 taggacgtaa tgtctggttt ataaaatgaa ccttcaattt tattttttat gaaaacagca
    25801 tttcattttt atggtttcgt ttataccgat ggtttatgtg gaaattgtcg aatagagcag
    25861 gtttgcgcaa cgctgggatc agtcttaaaa agtaaaaaaa tatatttgct tgaacgattc
    25921 accgtttttt tcatccggtt aaatatgcaa agataaatgc gcagaaatgt gtttctcaaa
    25981 ccgttcattt atcacaaaag gattgttcga tgtccaacaa tggctcgtca ccgctggtgc
    26041 tttggtataa ccaactcggc atgaatgatg tagacagggt tgggggcaaa aatgcctccc
    26101 tgggtgaaat gattactaac ctttccggaa tgggtgtttc cgttccgaat ggtttcgcca
    26161 caaccgccga cgcgtttaac cagtttctgg accaaagcgg cgtaaaccag cgcatttatg
    26221 aactgctgga taaaacggat attgacgatg ttacccagct tgcgaaagcg ggcgcgcaaa
    26281 tccgccagtg gattatcgac actcccttcc agcctgagct ggaaaacgcc atccgcgaag
    26341 cctatgcaca gctttccgcc gatgacgaaa acgcctcttt tgcggtgcgc tcctccgcca
    26401 ccgcagaaga tatgccggac gcttcctttg ccggtcagca ggaaaccttc ctcaacgttc
    26461 aggggtttga cgccgttctc gtggcagtga aacatgtatt tgcttctctg tttaacgatc
    26521 gcgccatctc ttatcgtgtg caccagggtt acgatcaccg tggcgtggcg ctctccgccg
    26581 gtgttcaacg gatggtgcgc tctgacctcg catcatctgg cgttatgttc tccattgata
    26641 ccgaatccgg ctttgaccag gtggtgttta tcacttccgc atggggcctt ggggaaatgg
    26701 tcgtgcaggg tgcggttaac ccggatgagt tttacgtcca taaaccgaca ctggcggcga
    26761 atcgcccggc tatcgtgcgc cgcaccatgg ggtcgaaaaa aatccgcatg gtttacgcgc
    26821 cgacccagga gcacggcaag caggttaaaa tcgaagacgt accgcaggaa cagcgtgaca
    26881 tcttctcgct gaccaacgaa gaagtgcagg aactggcaaa acaggccgta caaattgaga
    26941 aacactacgg tcgcccaatg gatattgagt gggcgaaaga tggccacacc ggtaaactgt
    27001 tcattgtgca ggcgcgtccg gaaaccgtgc gctcacgcgg tcaggtcatg gagcgttata
    27061 cgctgcattc acagggtaag attatcgccg aaggccgtgc tatcggtcat cgcatcggcg
    27121 cgggtccggt gaaagtcatc catgacatca gcgaaatgaa ccgcatcgaa cctggcgacg
    27181 tgctggttac tgacatgacc gacccggact gggaaccgat catgaagaaa gcatctgcca
    27241 tcgtcaccaa ccgtggcggt cgtacctgtc acgcggcgat catcgctcgt gaactgggca
    27301 ttccggcggt agtgggctgt ggagatgcaa cagaacggat gaaagacggt gagaaagtca
    27361 ctgtttcttg tgccgaaggt gataccggtt acgtctatgc ggagttgctg gaatttagcg
    27421 tgaaaagctc cagcgtagaa acgatgccgg atctgccgtt gaaagtgatg atgaacgtcg
    27481 gtaacccgga ccgtgctttc gacttcgcct gcctgccgaa cgaaggcgtg ggccttgcgc
    27541 gtctggaatt tatcatcaac cgtatgattg gcgtccaccc acgcgcactg cttgagtttg
    27601 acgatcagga accgcagttg caaaacgaaa tccgcgagat gatgaaaggt tttgattctc
    27661 cgcgtgaatt ttacgttggt cgtctgactg aagggatcgc gacgctgggt gccgcgtttt
    27721 atccgaagcg cgtcattgtc cgtctctctg attttaaatc gaacgaatat gccaacctgg
    27781 tcggtggtga gcgttacgag ccagatgaag agaacccgat gctcggcttc cgtggcgcgg
    27841 gccgctatgt ttccgacagc ttccgcgact gcttcgcgct ggagtgtgaa gcagtgaaac
    27901 gagtgcgcaa cgacatgggg ctgaccaacg ttgagatcat gatcccgttc gtgcgtaccg
    27961 tagatcaggc gaaagcggtg gttgaagaac tggcgcgtca ggggctgaaa cgtggcgaga
    28021 acgggctgaa aatcatcatg atgtgtgaaa ttccgtccaa cgccttgctg gcagagcagt
    28081 tcctcgaata tttcgacggc ttctcaattg gctcaaacga tatgacacag ctggcgctcg
    28141 gtctggatcg tgactccggc gtggtgtctg aattgttcga tgagcgcaac gatgcggtga
    28201 aagcattgct gtcgatggct atccgtgccg cgaagaaaca gggcaaatat gtcgggattt
    28261 gcggtcaggg tccgtccgac catgaagact ttgccgcatg gttgatggaa gaggggatcg
    28321 atagcctgtc tctgaacccg gacaccgtgg tgcaaacctg gttaagcctg gctgaactga
    28381 agaaataaaa taaatccccg gcggcgttca gtcgccgggt ttatgtgatc cccgaagatg
    28441 aaacttattc aatctcttca cagacatcct gcgttaatcg ccgcataata tcttttctta
    28501 acaaaaactt ttgtatttta cctgaggcag tgcgcggtag tttttcgatt accacgatat
    28561 gttcaggata tttatatttt gcgacccgtt tacggctaaa aaaagccacc acctcttcca
    28621 gcgataatga atgatgcggc gctttcagca cgacataagc gcatgatcgt tcacctaaac
    28681 gttcatcggg cattgcaacc acacaggcat cgtgaatttt aggatgctgc aataaaatat
    28741 cttccacttc acggctgcta atattttcgc cgccgcggac aataatatct tttttgcgtc
    28801 cggtaatttt tatatagcca gcctcatcca tacggcagag atcgccgctg taataccagc
    28861 cttcttcatc cagcgcacgg gcggttaatt caggttcatc aaaatatccc ataaacacat
    28921 tggggccacg cgaggcttct tcaccttcgc aacctggcgg taaggtcttg cgtgcgtcat
    28981 cgaccacttt aatctctaca cctgcggcag cgtaaccatc ggtgtgcata aagcgcgaca
    29041 aaggatcatc gagattcacc accgcatgcg gcgaactttc tgtggaacca taaacactta
    29101 ataatttaat gccgagctgc tggcattcac gcgccacttt tttggggatt gtggttccgc
    29161 cgcaaagaaa gaaacgcagc gctgacaggt ccgcgggttg tttctctagt acattcaaaa
    29221 gatcatagac aaacggcgtt gcgcctagca tacaggtgca acgctgctgc tcaagcagcg
    29281 cgagacacgc atcaggagtg aaaatatcta acaacacgct gcgagcacca attaaaaatg
    29341 gtgctgttac gccatgcaga aatcccgttg cgtgaccaag tggcgcaggc atcataaaga
    29401 catcctgcca ggtcagattc agccgcacgc aataagcccg ctcactggcg agaatattgt
    29461 tatgcgttag cattacgccc tttggcagac cctcggttcc ggaggtaaac agcaccgcag
    29521 ctaattcatc gccgtgggtc gttatcgccg tggtcagtga ggtattgtcg gcgataatct
    29581 gacttaatga gagggaagag gtggcgggag ccagtttgtc cacgccgaca atttgttgta
    29641 gttgtggaag ctgattttgc agcggcagga ttaaatctac cgggcgcgtt tgtttaaaca
    29701 acgtcggtgc aaagaacatt tttgcctgac acttattgag cacccacacc agttctgctt
    29761 cccgccagga aggcaacagc ggcacggaaa ccgcaccgat tttcaggcag gcaagataga
    29821 taacggtaaa ttcacaccag ccaggcagtt gaaatgcgat gcgatcgcct gattcaatac
    29881 ccttcgctaa catccagttt gccagacagc tcgcggcgtg atcgagcgcg ctataggtgt
    29941 acgatgcacc atgattatcg accacggcaa ttttgtctgg catcgcacga gcggtctgct
    30001 gccagtaatc ggccagcgaa gcatcgcccc atagcccttg ctgacgatac gccgcacgac
    30061 gttgttcgtt gaacgttaat gtcactttca tgttggactc tcgaaacaga acatcaggcc
    30121 cgagatgcgg gcctgtggga tgcatgatta gccgtagcga aaatcaatac cgaaggtgcc
    30181 tgcgggatat tgccactgtt cgagaatagt gttaccgcac agcacccgac aggtgccgca
    30241 ctccagacaa ccggcagaat caaaatgaat gttgccggcg tcatcctgct tataaagtcc
    30301 ggcagggcag gctttcatta atttacggaa ttcattgata tcgggatttg ccgccagaat
    30361 gatatgcggg tggccctcat caacatggaa tttattgacg cctaatttga tgtcgacgtt
    30421 aaccgtagcg ttctggctca tagcgcggtt gctcccttaa tgccatcttt cagcaagttg
    30481 atcagcccaa ttttcttcgc atgtcccatg atcattttgc gtaccggctg gtttggtttg
    30541 ccgtcaatgg tgaacatctc gttcatgatg tcggcgacca ttcgtgggta ttggctaaac
    30601 aggcgcgggt tttccatcag cgccgggatc ttgcggaaat gctgcatatc gcgcatgacg
    30661 cagctttgtt ccagctcacg tttgtattgc gccagactgc tggcggagaa atcctcgcgt
    30721 tctttggcgg cgatcaccgt tgtggcggca gcctgagccg atgcaatggc taaatccatg
    30781 ccacgaactg taaaacccaa attcaggcag aagcctgcgg cgtcaccaac gatcatcacg
    30841 ccatcgttaa ccatctgcgg caccattgcc agaccgcctt ctggcaccat atgcccggaa
    30901 tattcaagca gtttgccgcc gctaattagt gggcgaatgg cggggtgttg tttaaaatct
    30961 tccagcattt gcggcacgct tttttgcgca tgggcgatat cacccaatcc acaaaccagc
    31021 cccaaggata tggaatcctt gttggtatag agaaatcccc cgcccatcag gccgtcagaa
    31081 ggggaaccgg caaacagcca ggcggcacct tcctcgcccg taatattaaa gcgatcgttg
    31141 atctgttctg gtgtgaggcc aataacctct ttaacaccaa cagcgtaatg atgcggatcg
    31201 gaagcgggaa ccattcccag cgagcggcca agcatcgagt taacgccatc agccagaatc
    31261 accacattcg cttcgagaat atcatcccca gcctgcacgc cagtgacctt gtttccttca
    31321 cgaaccagcg catcgacgcg aacgcctggg ataaactgcg cgcccgcctg ctcggcttgt
    31381 tccatcaacc acgggtccag acgattacgc aatacggtat aagatgcgtg ttgcggaaca
    31441 tctggttgct cgcggtgaaa atcgagggta acggcgcttt cttcggttaa gaaggagatt
    31501 ttctcgcgtg tgaccttgcg ttctaccggc gctgatactg caaagcctgg aatgattgct
    31561 tcaagtgtgt gggcataaag acgcccgccg gtcatgtttt tacatccggc actgtcgccg
    31621 cgttctatca ccaggacatc cagcccggct cgcgccatga ccagtgcggc aacgctacca
    31681 gcaacgcccg caccgaccac aatggcatca aatttgtctt ccgacatgcg ttatgctccc
    31741 ttcccgctgc gtttacagcg ggagatgtgg ttgaaagggg aattagcggc tcaactggct
    31801 aatcagggca gggacgactt tgtagatatc gcccaccaga ccgtagtcgg cataattgaa
    31861 gattggcgca tttttatctt tattgatggc gacaatcact tttgcgccgt tgccgccaac
    31921 catatgctgg atctgcccgg agatccccag cgtcaggtag agatcggatt tcagcaacac
    31981 gccggagaca ccgatataac gttcacgctc catccagttc tcgccttcgg caattggacg
    32041 tgaacagccg acttcagcat tcagcaccgc cgccagttcg tggaccattt tcaggtcatc
    32101 ctgcgccgcc agaccacgac cgacgccaac cacacgtttt gctttgctca ggtccacgct
    32161 gcttgcggct ttcgcacggc gttcgcgaca gagaatttca tgacgcggag caacataagg
    32221 taccgtttct gtcgggcact gatgagaggt atcactggtg cacggttcct gaacaccggg
    32281 tgcaagggta atgatcgcca gcgggctgtt gatcttttcc tgagcgaacg ctaacccgcc
    32341 atacatccgg tgttcggcgc aaatgtgacc atcgataata tccaccgccg tggcatcgtt
    32401 caccagcgcc gcattcagtt gcacacttaa ccgtgctgcc agagctttac cacgtttcgt
    32461 cgcggccaac agcaacatag cggggtgttt atctttcagc agggcagcaa tgctttcggc
    32521 gtaattttca gtgcgttgca gcgcgtcgtt ttgctcaaga acataaatac attttggacc
    32581 ataaggcata actgcctgcg cctggtcggt attttgtaca atggcataca cctgttggcc
    32641 ccattgctga gcgccgccaa acagttcagc ataacgttca ggattatcgc taaagaccca
    32701 gacgctgttt aattgactca tcgtttcttc tccgtaggct tcagttcagg gctttcttca
    32761 gatgttccgc cagctcggca atggcctccg gcgaatcgtt atcgataatg atgtgcttac
    32821 gttctgtttg cggcggtacg cgaatgccga ccagttcagc aagtggcgcg ctctggctcc
    32881 agtcaatacc acttgcctgc cactgattta ccggtttttt acccgcgccg agaatggctt
    32941 tcatcgaagg aatgcgtggc acgttaatat cggaggtgac gcagagcacg gctggaacag
    33001 agagttcaat aacttcaaca tcatcttcaa gcgtgcgttc aatcaccagt gtattgccct
    33061 gacgctgaat agcactcact gcattaatca ccggaagttg cagaatttct ccgaccagca
    33121 agccagcctg ctgggcataa aggtcgccgg aaccttcacc aaagatcagt aaatcgaagc
    33181 cgatcttttc aactgctgct gccagcgctt ttgcggtatc gagaggcagt gcatgttcaa
    33241 gttgcgcatc ctgcaccaaa tacaggctgt gcggcccgcg ggatagcacg tctttgcgca
    33301 ctttcgagtt ctgcaacaat gagccgccaa cggtcagcgc ggctatctca tcgtcatctg
    33361 ttgcaagctg gcttgcagct tcaatggcat tgagatcgaa ctggctgatt ttggcgtcgg
    33421 cattgtcgaa attcagggtg tattctggag tgacaacaat gtcctgttct tcaggcacca
    33481 gcttaaagca ggttattatt ttcatgattt ctcctgtgag tcgcatacgg aaccatctcc
    33541 gatggaaggt aaaaatggcc tgaaaatagt gtgcgatagc ggtgcggggt tttcgacgga
    33601 gcaagctgtt ttccttgcca acaatcttgc aaaaaaaatg cgatcgctaa aacaaaagtg
    33661 caatttgcgg caatatagtt attccatgac cgtacaatct tgcattcagt gtaaataatc
    33721 ttgtttagct ctcgctaaac gcggttgtat aaccgcaata cacgccgtat tttatccggc
    33781 atatattgtt tttgcttgct catggaatag ggaggttgtc atgtatcaac gctgttttga
    33841 taatgccagt gaaacgctgt ttgtcgccgg taaaacgcca cggctttcac gttttgcaat
    33901 tagcgatgat ccaaaatggg agtctggaca tcacgttcat gacaatgaaa ccgagctgat
    33961 ttacgtcaag aaaggggtag caaggtttac catcgattct tcgttatatg tcgcacatgc
    34021 ggatgacatt gtggtgatag aacgcggcag gctgcatgcg gtggcctctg acgttaacga
    34081 tccggcaacg acgtgtacct gtgcgctgta cggctttcag tttcaggggg ctgaggaaaa
    34141 tcagctactg caaccgcatt cttgtccggt aattgccgcg gggcagggaa aagaagtcat
    34201 aaaaacctta tttaatgagc taagtgtgat tttgccgcaa agtaaaaata gccaaacatc
    34261 ttcgttatag gatgcatttg cctatacgtt agccattctt tactacgaaa attttaaaaa
    34321 tgctta
//
LOCUS       DAZFXT010000044        34273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.044, whole genome shotgun sequence.
ACCESSION   DAZFXT010000044 DAZFXT010000000
VERSION     DAZFXT010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.044"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(312..524)
                     /gene="rpmE"
                     /locus_tag="ACHKW0_002220"
     CDS             complement(312..524)
                     /gene="rpmE"
                     /locus_tag="ACHKW0_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HFO7475051.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            727..2925
                     /gene="priA"
                     /locus_tag="ACHKW0_002221"
     CDS             727..2925
                     /gene="priA"
                     /locus_tag="ACHKW0_002221"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HFO7475052.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKEQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWQTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3081..4106
                     /gene="cytR"
                     /locus_tag="ACHKW0_002222"
     CDS             3081..4106
                     /gene="cytR"
                     /locus_tag="ACHKW0_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HFO7475053.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4198..5157
                     /gene="ftsN"
                     /locus_tag="ACHKW0_002223"
     CDS             4198..5157
                     /gene="ftsN"
                     /locus_tag="ACHKW0_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HFO7475054.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQADTVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5250..5780
                     /gene="hslV"
                     /locus_tag="ACHKW0_002224"
     CDS             5250..5780
                     /gene="hslV"
                     /locus_tag="ACHKW0_002224"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HFO7475055.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5790..7121
                     /gene="hslU"
                     /locus_tag="ACHKW0_002225"
     CDS             5790..7121
                     /gene="hslU"
                     /locus_tag="ACHKW0_002225"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HFO7475056.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7188..8114
                     /gene="menA"
                     /locus_tag="ACHKW0_002226"
     CDS             7188..8114
                     /gene="menA"
                     /locus_tag="ACHKW0_002226"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HFO7475057.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8207..8692
                     /gene="rraA"
                     /locus_tag="ACHKW0_002227"
     CDS             8207..8692
                     /gene="rraA"
                     /locus_tag="ACHKW0_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008428 - ribonuclease inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0051252 - regulation of RNA metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HFO7475058.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="ACHKW0_002228"
     CDS             complement(8777..9022)
                     /gene="zapB"
                     /locus_tag="ACHKW0_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HFO7475059.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9447..10292
                     /gene="glpF"
                     /locus_tag="ACHKW0_002229"
     CDS             9447..10292
                     /gene="glpF"
                     /locus_tag="ACHKW0_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HFO7475060.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10315..11823
                     /gene="glpK"
                     /locus_tag="ACHKW0_002230"
     CDS             10315..11823
                     /gene="glpK"
                     /locus_tag="ACHKW0_002230"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HFO7475061.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            11959..12969
                     /gene="glpX"
                     /locus_tag="ACHKW0_002231"
     CDS             11959..12969
                     /gene="glpX"
                     /locus_tag="ACHKW0_002231"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HFO7475062.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13066..13812
                     /gene="fpr"
                     /locus_tag="ACHKW0_002232"
     CDS             13066..13812
                     /gene="fpr"
                     /locus_tag="ACHKW0_002232"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004324 - ferredoxin-NADP+ reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HFO7475063.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="ACHKW0_002233"
     CDS             complement(13817..14245)
                     /gene="uspD"
                     /locus_tag="ACHKW0_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HFO7475064.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14272..14571)
                     /gene="yiiS"
                     /locus_tag="ACHKW0_002234"
     CDS             complement(14272..14571)
                     /gene="yiiS"
                     /locus_tag="ACHKW0_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HFO7475065.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14783..15223)
                     /gene="yiiR"
                     /locus_tag="ACHKW0_002235"
     CDS             complement(14783..15223)
                     /gene="yiiR"
                     /locus_tag="ACHKW0_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7475066.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15324..15923
                     /gene="yiiQ"
                     /locus_tag="ACHKW0_002236"
     CDS             15324..15923
                     /gene="yiiQ"
                     /locus_tag="ACHKW0_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HFO7475067.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16031..16798
                     /gene="tpiA"
                     /locus_tag="ACHKW0_002237"
     CDS             16031..16798
                     /gene="tpiA"
                     /locus_tag="ACHKW0_002237"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HFO7475068.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="ACHKW0_002238"
     CDS             complement(16853..17608)
                     /gene="cdh"
                     /locus_tag="ACHKW0_002238"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008715 - CDP-diacylglycerol diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HFO7475069.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="ACHKW0_002239"
     CDS             complement(17715..18704)
                     /gene="sbp"
                     /locus_tag="ACHKW0_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HFO7475070.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="ACHKW0_002240"
     CDS             complement(19024..19986)
                     /gene="pfkA"
                     /locus_tag="ACHKW0_002240"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HFO7475071.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="ACHKW0_002241"
     CDS             complement(20167..21069)
                     /gene="fieF"
                     /locus_tag="ACHKW0_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HFO7475072.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21218..21718)
                     /gene="cpxP"
                     /locus_tag="ACHKW0_002242"
     CDS             complement(21218..21718)
                     /gene="cpxP"
                     /locus_tag="ACHKW0_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HFO7475073.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21868..22566
                     /gene="cpxR"
                     /locus_tag="ACHKW0_002243"
     CDS             21868..22566
                     /gene="cpxR"
                     /locus_tag="ACHKW0_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HFO7475074.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22563..23936
                     /gene="cpxA"
                     /locus_tag="ACHKW0_002244"
     CDS             22563..23936
                     /gene="cpxA"
                     /locus_tag="ACHKW0_002244"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HFO7475075.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24042..24716)
                     /gene="yiiM"
                     /locus_tag="ACHKW0_002245"
     CDS             complement(24042..24716)
                     /gene="yiiM"
                     /locus_tag="ACHKW0_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxyaminopurine reductase"
                     /protein_id="HFO7475076.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24865..25848)
                     /gene="kdgT"
                     /locus_tag="ACHKW0_002246"
     CDS             complement(24865..25848)
                     /gene="kdgT"
                     /locus_tag="ACHKW0_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015649 - 2-keto-3-deoxygluconate:proton
                     symporter activity [Evidence IEA];
                     GO_process: GO:0046411 - 2-keto-3-deoxygluconate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HFO7475077.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26108..26728)
                     /gene="sodA"
                     /locus_tag="ACHKW0_002247"
     CDS             complement(26108..26728)
                     /gene="sodA"
                     /locus_tag="ACHKW0_002247"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HFO7475078.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27013..28047
                     /gene="rhaT"
                     /locus_tag="ACHKW0_002248"
     CDS             27013..28047
                     /gene="rhaT"
                     /locus_tag="ACHKW0_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015153 - rhamnose transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008645 - hexose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HFO7475079.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWAISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGLSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     IIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNAGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28044..28892)
                     /gene="rhaR"
                     /locus_tag="ACHKW0_002249"
     CDS             complement(28044..28892)
                     /gene="rhaR"
                     /locus_tag="ACHKW0_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HFO7475080.1"
                     /translation="MAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDFCELVIV
                     WRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKLNLDWQG
                     AIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVSFANEMAELLFGQLVMLLN
                     RHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQFRQQTG
                     MTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMTPSQWRH
                     LNSQKD"
     gene            complement(28966..29802)
                     /gene="rhaS"
                     /locus_tag="ACHKW0_002250"
     CDS             complement(28966..29802)
                     /gene="rhaS"
                     /locus_tag="ACHKW0_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HFO7475081.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVAEQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30090..31559
                     /gene="rhaB"
                     /locus_tag="ACHKW0_002251"
     CDS             30090..31559
                     /gene="rhaB"
                     /locus_tag="ACHKW0_002251"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008993 - rhamnulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HFO7475082.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            31556..32815
                     /gene="rhaA"
                     /locus_tag="ACHKW0_002252"
     CDS             31556..32815
                     /gene="rhaA"
                     /locus_tag="ACHKW0_002252"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008740 - L-rhamnose isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HFO7475083.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33057..33881
                     /gene="rhaD"
                     /locus_tag="ACHKW0_002253"
     CDS             33057..33881
                     /gene="rhaD"
                     /locus_tag="ACHKW0_002253"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HFO7475084.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            33891..34205
                     /gene="rhaM"
                     /locus_tag="ACHKW0_002254"
     CDS             33891..34205
                     /gene="rhaM"
                     /locus_tag="ACHKW0_002254"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019299 - rhamnose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HFO7475085.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         8378 a         8932 c         8904 g         8059 t
ORIGIN      
        1 ggattgaccg gatggccgga cgtcaccccg ccggggcaca ccgaacaggc cacgccggtg
       61 ggggcaccga tgcgcacccc ggctgaaccc tggacaatgc taccgccata ctgcgtcatg
      121 tcgccctgac gcgccgccgg ttttccgctc atcgtaaaat ccttttgaaa taaataagaa
      181 ccagaagcct agcgccagtc aacaacagga aatgataatg agttaacacc ccggaatttt
      241 tcagaattta taaaaggcaa attacaggca caaaaaaagc gccgtgcggc gcttttttcg
      301 gaaatccggt cttatttgct gcccgggatg ttgaaacgct tgttgaagcg gtcaacacgg
      361 ccaccggtag caacatcacg ctgtttgcca gtgaagaacg ggtggcactt gctgcacacg
      421 tcgaggttca ggtcatgacc aacggtggag cggattttca ttacgttacc gcaagagcag
      481 ctagcagtaa tttcttcgta tttcgggtga atatcttttt tcatgggaaa acctcggttt
      541 aaggccgcgt cgctcttcca gccctaacgc cagacaccac gcgatgttaa aagtatagct
      601 tcaatacgat catttcgtac gaaggcgcga aatcatacag aaattaacca gcgtatgcaa
      661 actgatccgc actcttctac ggcaatgtgt atactaaccc accgaatttc aagtcaggat
      721 gatgctatgc ccgttgccca cgttgccttg cccgttccgc ttcctcgtac ctttgactat
      781 ctgctgccag aaggcatgac ggttaaagcc gggtgtcgcg tgcgcgtgcc gtttggcaaa
      841 gagcaggagc gcatcggggt tgtggtgtca gttagcgatg tcagtgagct gccgcttaac
      901 gagctgaaag cggtagtgga agtgctggac gttgagccgg tatttactca ctccgtctgg
      961 cggttactgc tctgggcggc agattactat catcatccga ttggcgatgt gctgtttcat
     1021 gccttgccga ttttactgcg ccaggggcgg cctgcggcga acgcgccgat gtggtactgg
     1081 tttgccactg aacaaggcca ggcggtggat ctgaacagcc tgaaacgctc ccccaagcaa
     1141 caacaggcgc tggcggcgtt acggcaaggc aaaatctggc gcgaccaggt cgccacgctc
     1201 gaatttaatg atgccgcgtt gcaggcgcta cgcaaaaaag gtctgtgtga tttagcaagt
     1261 gaaacaccag agtttagcga ctggcaaacg aactatgccg tttctggtga gcggttgcga
     1321 ttgaataccg aacaggccac cgccgttggc gcaattcata gcgcggcaga tactttttct
     1381 gcctggctgc tggcgggcgt taccggttcc ggtaaaacgg aggtttatct cagtgtactg
     1441 gaaaacgtgc tcgctcaggg caaacaggcg ctggtgatgg tgccggaaat cggcctgaca
     1501 ccgcaaacta tcgcccgttt tcgtgaacgt tttaatgccc ccgtggaagt tctgcattcc
     1561 ggcctgaacg acagcgagcg gctttcggcg tggttgaaag cgaaaaatgg cgaggcggcg
     1621 attgtgatcg gcacccgctc cgcgctgttt acgccgttta aaaatctcgg cgtgattgtc
     1681 attgatgaag agcacgacag ctcctacaag cagcaggaag gctggcgcta tcatgcccgc
     1741 gacctggcgg tgtatcgtgc gcatagcgag caaatcccga ttattcttgg ctccgcaacg
     1801 cccgcgctgg aaacgttgtg caacgtccag cagaaaaaat accgcctgct gcgcctgacg
     1861 cgccgggcgg ggaatgcgcg tccggcaatt caacacgtgc tggatttaaa aggtcagaag
     1921 gtgcaggcag gtctggctcc ggcgttaatc acccgtatgc gccagcattt acaggccaac
     1981 aatcaggtta tcctctttct taatcgtcgg ggttttgcgc ctgcgctact gtgccacgac
     2041 tgcggctgga ttgccgaatg cccacgttgc gatcactact acacgctgca tcaggcgcaa
     2101 cagcatttgc gctgtcacca ctgtgacagt cagcgtccgg tgccgcgcca gtgcccttcc
     2161 tgcggttcca cgcacctggt acccgtgggg ctgggcaccg aacagcttga acagacgctt
     2221 gcgccgctgt tccctgatgt gcccatttct cgtatcgacc gcgataccac cagccgcaaa
     2281 ggggcgctgg aacagcaact ggcggaagta catcgcggcg gtgcgaggat tttgatcggc
     2341 acacaaatgc tggcgaaagg tcaccacttt ccggatgtga cgctggtggc gttactggat
     2401 gtggacggcg cgctgttttc tgccgatttt cgctcggcag agcgtttcgc tcagctttac
     2461 acccaggtcg ctggtcgtgc cgggcgtgcg ggtaaacaag gcgaagtggt gctgcaaacg
     2521 caccatccag aacatcctct gttgcaaacg ttgctctata aaggctacga cgcctttgcc
     2581 gaacaggcgc tggctgagcg acggatgatg caactgccgc catggaccag ccatgtgatt
     2641 gtgcgtgcgg aagatcataa caatcagcat gcgccgctgt tcctgcaaca actgcgtaat
     2701 ctgatcctct ccagcccgct ggcagacgac aaactgtggg ttctcggtcc ggttccggca
     2761 ctggcaccta aacgtggcgg tcgctggcgc tggcagatat tgttgcagca cccttcccgc
     2821 gtgcgcttgc aacacatcat tagtggtacg ctggcgctca tcaatacaat accggattcc
     2881 cgcaaggtga aatgggtact ggatgttgat ccgattgagg gttaaaccgc ttacgatgcg
     2941 aggcggatcg aaaaattcaa tattcatcac acttttcatg aaaattctgt aaccgttttc
     3001 acgcgctatc tgctaaaaat gttgccgatg tgaagtaaac atggatgtag tacgcctgac
     3061 gtgccaggcg aggagtgagt gtgaaagcga agaagcagga aactgccgcg accatgaaag
     3121 acgttgccct caaggcaaaa gtctctacag cgactgtctc ccgagcatta atgaatcccg
     3181 ataaagtctc ccaggccacc cgtaatcggg ttgaaaaagc ggcccgggaa gtgggttatt
     3241 taccgcagcc tatggggcgc aacgtcaagc gtaatgaatc ccgcaccatt ctggtgattg
     3301 tcccggatat ctgcgatccc ttctttagcg aaattattcg cggtatcgaa gttacggcgg
     3361 caaatcacgg atatctggtg ctgattggcg actgtgcgca tcaaaatcag caggaaaaaa
     3421 cctttatcga tttgatcatc accaagcaaa ttgatggcat gttgctgctg ggttcaaggc
     3481 tgccgtttga tgccagcatt gaggaacagc gtaatctgcc gccgatggtg atggcgaacg
     3541 aattcgcacc ggaactggag ctacctacag ttcatatcga caatctgacc gccgcatttg
     3601 atgcagtaaa ttatctatat gagcaagggc ataaacggat tggctgtata gccggtcccg
     3661 aagagatgcc gctgtgtcac taccgcctgc aaggttacgt tcaggcgctg cgtcgctgcg
     3721 gcattatggt tgatccgcaa tacatcgccc gtggcgactt caccttcgaa gccggaagca
     3781 aagcgatgca gcagctgctt gatcttccac aaccgcctac tgctgtcttc tgccatagcg
     3841 atgtgatggc gctcggcgca ctttctcagg caaaacgcca ggggctgaaa gtcccggaag
     3901 acctttccat aatcggtttt gataacatcg acctgacgca attttgtgat ccgccgctga
     3961 caaccatcgc gcagccgcgt tacgaaatcg gtcgggaagc tatgctgtta ttgcttgatc
     4021 aaatgcaggg gcaacacgtt ggcagtggct ctcgtttaat ggactgcgaa cttatcatcc
     4081 ggggatcaac acgcgcgtta ccttaaagta aacctttaag actgccgtga ctggtcaaag
     4141 gcccgccgct taagtaacat ggcgggctga cgaacgaata aatacagcga aacgatagtg
     4201 gcacaacgag attatgtacg ccgcagccaa ccggcacctt cgcggcgaaa aaagagcacc
     4261 tcacggaaaa agcaacgaaa tctgcctgcg gtttctcccg ctatggtcgc tattgctgcc
     4321 gccgttcttg tgacctttat cggtggtctg tacttcatta cgcatcacaa gaaagaagag
     4381 tccgagacgc tgcaaagcca gaaagtgacc ggtaacggac taccaccaaa accagaagaa
     4441 cgctggcgct acattaaaga gctggaaagt cgccagccgg gtgtgcgtgc gcccacagaa
     4501 ccttctgccg gtggtgaagt gaaaacgccg gagcaactga caccagaaca acgtcagctt
     4561 cttgaacaaa tgcaggctga tatgcgccag cagccaacgc agctggttga agttccgtgg
     4621 aatgaacaga cgccggaaca gcgtcagcaa acgctacaac gccagcgtca ggcgcagcaa
     4681 ttagcggagc agcaacgcct ggctcagcag tcgcgtacga ctgaacaaag ctggcagcag
     4741 cagacgcgta cgtcgcaagc cgcgccggta caggcgcaac cgcgccagtc taaaccggct
     4801 tctactcagc agccgtacca ggatctgctg caaacccctg cgcacacgac cgcgcagtcg
     4861 aagccgcagc aagctgcgcc ggttgctcgt gccgctgacg cgccaaaacc gacggcggag
     4921 aaaaaagacg aacgccgctg gatggtgcag tgcggttcgt tcagaggcgc ggaacaggca
     4981 gatacggtac gtgctcagct ggcgttcgaa ggatttgact cgaaaatcac caccaacaat
     5041 ggctggaatc gtgtggtcat tggcccggtg aaaggcaaag agaacgcaga cagcaccctc
     5101 aatcggttga agatggcggg tcatacaaac tgcattcggc tcgccgccgg gggttgaaac
     5161 cctcaaaatc ccccccatct ataattgcat tatgccccgt acttttgtac ggggtttgta
     5221 ctctgtattc gtaaccaagg ggtcagctcg tgacaactat agtaagcgta cgccgtaacg
     5281 gccatgtggt catcgctggt gatggtcagg ccacgttggg caataccgta atgaaaggca
     5341 acgtgaaaaa ggtccgccgt ctgtacaacg acaaagtcat cgcgggcttt gcgggcggta
     5401 ctgcggatgc ttttacgctg ttcgaactgt ttgaacgtaa actggaaatg catcagggcc
     5461 atctggtcaa agccgccgtt gagctggcga aagactggcg taccgatcgc atgctgcgca
     5521 aacttgaagc actgctggca gtcgcggatg aaactgcatc gcttatcatc accggtaacg
     5581 gtgacgtggt gcagccagaa aacgatctta ttgctatcgg ctccggcggc ccttacgccc
     5641 aggctgcggc gcgtgcactg ttagaaaata ctgaactgag tgctcgtgaa attgctgaaa
     5701 aggcgttgga tattgcaggc gacatttgca tctataccaa ccatttccac accatcgaag
     5761 aattaagcta caaagcgtaa ggatctccca tgtctgaaat gaccccacgc gaaatcgtca
     5821 gcgaactgga taagcacatc atcggccagg acaacgccaa gcgttctgtg gcgattgctc
     5881 tgcgtaaccg ctggcgtcgc atgcagctca acgaagagct gcgccatgaa gtgaccccga
     5941 aaaatatcct gatgatcggc ccgaccggtg tcggtaaaac tgaaatcgcc cgtcgtctgg
     6001 ctaagctggc gaatgcgccg ttcatcaaag ttgaagcgac caaattcacc gaagtgggct
     6061 acgtcggtaa ggaagtggat tctattattc gcgatctgac cgatgccgcc gtgaaaatgg
     6121 tacgcgtcca ggctatcgag aaaaaccgtt atcgcgctga agaactggca gaagaacgta
     6181 ttctcgacgt gctgatccca cctgctaaaa acaactgggg acagaccgaa cagcagcagg
     6241 aaccgtccgc tgctcgtcag gcattccgca aaaaactgcg tgaaggccag cttgatgaca
     6301 aagaaatcga gatcgatctt gccgcagcac cgatgggcgt tgaaattatg gctcctccag
     6361 gcatggaaga gatgaccagc cagctgcagt ccatgttcca gaacctgggc ggccagaagc
     6421 aaaaagcgcg taagctgaaa atcaaagacg ccatgaagct gctgattgaa gaagaagcgg
     6481 cgaaactggt gaacccggaa gagctgaagc aagacgctat cgacgctgtt gagcagcacg
     6541 ggatcgtgtt tatcgacgaa atcgacaaaa tctgtaagcg cggcgagtct tccggtccgg
     6601 atgtttctcg tgaaggcgtt cagcgtgacc tgctgccgct ggtagaaggt tgcaccgttt
     6661 ccaccaaaca cgggatggtc aaaactgacc acattctgtt tatcgcttct ggcgcgttcc
     6721 agattgcgaa accgtctgac ctgatcccgg aactgcaagg tcgtctgcca atccgcgttg
     6781 aactgcaggc gctgaccacc agcgacttcg agcgtattct gaccgagccg aatgcctcta
     6841 tcaccgtgca gtacaaagca ctgatggcga ctgaaggcgt aaatatcgag tttaccgact
     6901 ccggtattaa gcgcatcgcg gaagcggcat ggcaggtgaa cgaatctacc gaaaacatcg
     6961 gtgctcgtcg tttacacact gttctggagc gtttaatgga agagatttcc tacgacgcca
     7021 gcgatttaag cggtcaaact atcatcattg acgcagatta tgtgagcaaa catctggatg
     7081 cgttggtggc agatgaagat ctgagccgtt ttatcctata atcgcgttca atcattttca
     7141 tcattgtttg atggggctga aaggccccat ttttattggc gcgtattatg actgaacaac
     7201 aaattagccg aactcaggcg tggctggaaa gtttacgacc taaaaccctc cccctcgcct
     7261 ttgctgcaat tatcgtcggg acagcgctgg catggtggca aggtcacttc gatccgctgg
     7321 tcgccctgct ggcactgatt accgccgggc tattacagat cctttctaac ctcgccaatg
     7381 attacggcga tgcggtaaaa ggcagcgata aacctgaccg cattgggccg ctacgcggca
     7441 tgcaaaaagg ggtcattacc cagcaagaga tgaaacgggc gctcattatt accgtcgtgc
     7501 tcatctgtct ctccgggctg gcactggttg cagtggcatg ccatacgctg gccgattttg
     7561 tcggtttcct gattcttggc gggttgtcga tcattgccgc tatcacctac accgtgggca
     7621 atcgtcctta tggttatatc ggtctgggtg atatttccgt actggttttc tttggctggt
     7681 tgagtgtcat ggggagctgg tatttacagg ctcatacatt gattccggca ctgatccttc
     7741 cggcgaccgc atgcggcctg ctggcaacgg cagtactgaa tattaataac ctgcgtgata
     7801 tcaatagcga ccgcgaaaat ggcaaaaaca cgctggtggt gcgcttaggt gaagtgaacg
     7861 cgcgtcgtta tcatgcctgc ctgctgatgg gctcgctggt gtgtctggcg ctgtttaatc
     7921 tcttttcgct gcatagcctg tggggctggc tgttcctgct ggcggcacca ttactggtga
     7981 agcaagcccg ttatgtgatg cgggaaatgg acccggtggc gatgcgacca atgctggaac
     8041 gtactgtcaa gggagcgtta ctgactaacc tgctgtttgt tttagggata ttcctaagcc
     8101 agtgggcagc ataactgaca aatatcaatt aacaattgat gattttgcca acagcccaca
     8161 tagcgcgata tactgaaaat tctcgcagca actgaatgtt aagcctatga aatacgatac
     8221 ttccgagctt tgtgacatct atcaagaaga tgttaacgtc gtggaaccgc tgttctccaa
     8281 ctttggcgga cgggcgtcgt ttggcggaca aataatcacg gtaaaatgtt tcgaggacaa
     8341 cgggttgctg tacgatctgc tcgaacagaa tggccgtggt cgtgttcttg tcgtcgatgg
     8401 cggtggttct gttcgtcgcg cactggtcga tgctgaactg gcgcgtctgg cagtacaaaa
     8461 tgaatgggaa ggtctggtca tttacggcgc ggtgcgtcag gtagatgacc tggaagagtt
     8521 ggatatcggc atccaggcga tggcggcaat tccggttggt gccgctggcg aaggcattgg
     8581 cgaaagcgat gtccgcgtca attttggcgg tgtcaccttc ttctccggcg accatcttta
     8641 tgccgacaat accgggatta ttctttctga agatccgctg gatattgaat gatagaaaag
     8701 gcaccgcaag gtgccttttt tctgcgttac ctgttggcct acacagtaaa gaaattacgc
     8761 ggaagatgaa gcgtaatcag acctcttcca tgcgacccag cagggcctgc agacgttcct
     8821 gccagccgtt ctgctgttct ttcagatggt tgttctcacg ctccagctct tcgcgctgat
     8881 gctgggcatt ttgaacttcc tgcgacagtg agttgttttt ttctttcagc tcttcgattt
     8941 ccatctgcaa cagagtgatg gtatcaatcg cctgctgtac ttttgcttcc agtttctcaa
     9001 acacttctaa tgacattgtc atacctctcc tgaattgcaa ggcgttgatg gataaaaatc
     9061 ctcgtcccga ttaccggtga cgccttaata aatacgagcg cactttagtt agctccgatt
     9121 gtatgaagcc gcgccatcgc tgtccagcgg cacgccttgc agattacggt ttgccacact
     9181 tttcatcctt ctcctggtga cataatccac atcaatcgaa aatgttaata aatttgttgc
     9241 gcgaatgatc taacaaacat gcatcatgta caatcagatg gaataaatgg cgcgataacg
     9301 ctcattttat gacgaagcac acacatttta agttcgatat ttctcgtttt tgctcgttaa
     9361 cgataagttt acagcatgcc tacaagcatc gtggaggtcc gtgactttca cgcatacaac
     9421 aaacattaac tcttcaggat ccgattatga gtcaaacatc aaccttgaaa ggccagtgca
     9481 ttgctgaatt cctcggtacc gggttgttga ttttcttcgg tgtgggttgc gttgcagcac
     9541 taaaagtcgc tggtgcgtct tttggtcagt gggaaatcag tgtcatttgg ggactggggg
     9601 tggcaatggc catctacctg accgcagggg tttccggcgc gcatcttaat cccgctgtta
     9661 ccattgcatt gtggctgttt gcctgtttcg acaagcgcaa agttattcct tttatcgttt
     9721 cacaagttgc cggcgctttc tgtgctgcgg ctttagttta cgggctttac tacaatttat
     9781 ttttcgactt cgagcagact catcacattg ttcgcggcag cgttgaaagt gttgatctgg
     9841 ctggcacttt ctctacttac cctaatcctc atatcaattt tgtgcaggct ttcgcagttg
     9901 agatggtgat taccgctatt ctgatggggc tgatcctggc gttaacggac gatggcaacg
     9961 gtgtaccacg cggccctttg gctcccttgc tgattggtct actgattgcg gtcattggcg
    10021 catctatggg cccattgaca ggttttgcca tgaacccagc gcgtgacttc ggtccgaaag
    10081 tctttgcctg gctggcgggc tggggcaatg tcgcctttac cggcggcaga gacattcctt
    10141 acttcctggt gccgcttttc ggccctatcg ttggcgcgat tgtaggtgca tttgcctacc
    10201 gcaaactgat tggtcgccat ttgccttgcg atatctgtgt tgtggaagaa aaggaaacca
    10261 caactccttc agaacaaaaa gcttcgctgt aatatgacta cgggacaatt aaacatgact
    10321 gaaaaaaaat atatcgttgc gctcgaccag ggcaccacca gctcccgcgc ggtcgtaatg
    10381 gatcacgatg ccaatatcat tagcgtgtcg cagcgcgaat ttgagcaaat ctacccaaaa
    10441 ccaggttggg tagaacacga cccaatggaa atctgggcca cccaaagctc cacgctggta
    10501 gaagtgctgg cgaaagccga tatcagttcc gatcaaattg cagctatcgg tattacgaac
    10561 cagcgtgaaa ccactattgt ctgggaaaaa gaaaccggca agcctatcta taacgccatt
    10621 gtctggcagt gccgtcgtac cgcagaaatc tgcgagcatt taaaacgtga cggtttagaa
    10681 gattatatcc gcagcaatac cggtctggtg attgacccgt acttttctgg caccaaagtg
    10741 aagtggatcc tcgaccatgt ggaaggctct cgcgagcgtg cacgtcgtgg tgaattgctg
    10801 tttggtacgg ttgatacgtg gcttatctgg aaaatgactc agggccgtgt ccatgtgacc
    10861 gattacacca acgcctctcg taccatgttg ttcaacatcc ataccctgga ctgggacgac
    10921 aaaatgctgg aagtgctgga tattccgcgc gagatgctgc cagaagtgcg tcgttcttcc
    10981 gaagtatacg gtcagactaa cattggcggc aaaggcggca cgcgtattcc aatctccggg
    11041 atcgccggtg accagcaggc cgcgctgttt ggtcagttgt gcgtgaaaga agggatggcg
    11101 aagaacacct atggcactgg ctgctttatg ctgatgaaca ctggcgagaa agcggtgaaa
    11161 tcagaaaacg gcctgctgac caccatcgcc tgcggcccga ctggcgaagt gaactatgcg
    11221 ttggaaggtg cggtgtttat ggcaggcgca tccattcagt ggctgcgcga tgagatgaag
    11281 ttgattaacg acgcctacga ttccgaatat ttcgccacca aagtgcaaaa caccaatggt
    11341 gtgtatgtgg ttccggcatt taccgggctg ggtgcgccgt actgggaccc gtatgcgcgc
    11401 ggggcgattt tcggtctgac tcgtggggtg aacgctaacc acattatacg cgcgacgctg
    11461 gagtctattg cttatcagac gcgtgacgtg ctggaagcga tgcaggccga ctctggtatc
    11521 cgtctgcacg ccctgcgcgt ggatggtggc gcagtagcaa acaatttcct gatgcagttc
    11581 cagtccgata ttctcggcac ccgcgttgag cgcccggaag ttcgcgaagt gactgcattg
    11641 ggtgcggcct atcttgctgg tctggcggtc ggtttctggc agaacctcga cgagctgcaa
    11701 gagaaagcgg tgattgaacg cgagttccgt ccaggcatcg aaaccactga gcgtaattac
    11761 cgttacgcag gctggaaaaa agcggtcaaa cgcgcgatgg cgtgggaaga acacgacgag
    11821 taatgtaaat gccggatgaa gcgtttatgc cgcatccggt agtcctgaaa ccgtgcgggg
    11881 gcaaccccgc acacatcaat aatcccaccc ttcccctgtg ctacacttcg cgccattcct
    11941 tactgcttag agtttgctat gagacgagaa cttgccatcg aattttcccg cgtcaccgaa
    12001 tctgcggcgc tggctggcta caaatggtta ggacgcggcg ataaaaacac cgcggacggc
    12061 gcggcggtaa acgccatgcg tattatgctc aaccaggtca acattgacgg caccatcgtc
    12121 attggtgaag gtgaaatcga cgaagcaccg atgctctaca ttggtgaaaa agtcggtact
    12181 ggtcgcggcg acgcggtaga tattgctgtt gatccgattg aaggcacgcg catgacggcg
    12241 atgggccagg ctaacgcgct ggcggtgctg gcagtaggcg ataaaggctg cttcctcaat
    12301 gcgccggata tgtatatgga gaagctgatt gtcgggccgg gagccaaagg caccattgat
    12361 ctgaacctgc cgctggcgga taacctgcgc aatgtagcgg cggcgctcgg caaaccgttg
    12421 agcgaactga cggtaacgat tctagctaaa ccacgccacg atgccgttat cgctgaaatg
    12481 cagcaactcg gcgtacgcgt atttgctatt ccggatggcg acgttgcggc ctcaattctc
    12541 acctgtatgc cagacagcga agttgacgtg ctgtacggta ttggtggcgc gccggaaggc
    12601 gtagtttctg cggcggtgat ccgcgcatta gatggcgaca tgaacggtcg tctgctggcg
    12661 cgtcatgacg tcaaaggcga caacgaagag aatcgtcgca ttggcgagca ggagctggca
    12721 cgctgcaaag cgatgggcat cgaagccggt aaagtattgc gcctgggcga tatggcgcgc
    12781 agcgataacg tcatcttctc tgccaccggt attaccaaag gcgatctgct ggaaggcatt
    12841 agccgcaaag gcaatatcgc gactaccgaa acgctgctga tccgcggcaa gtcacgcacc
    12901 attcgccgca ttcagtccat ccactatctg gatcgcaaag acccggaaat gcaggtgcac
    12961 atcctctgat tgatttgatc gattgagcct tccagtcctt cgggactgga atttttttgt
    13021 tcggagaacg aagataaggc aagttaatca aaacaggaga aaaacatggc tgattgggta
    13081 acaggcaaag tcactaaagt gcagaactgg accgacgccc tgtttagtct caccgttcac
    13141 gcccccgtgc ttccgtttac cgccgggcaa ttcaccaagc ttggccttga aatcgacggc
    13201 gaacgcgtcc agcgcgccta ctcctatgtt aactcgcccg ataatcccga tctggagttt
    13261 tacctggtca ccgtccccga tggcaaatta agcccacgat tggcggcact gaaaccaggc
    13321 gatgaagtgc aggtggttag cgaagcagct ggcttctttg ttctggatga agtaccggac
    13381 tgcgaaacgc tatggatgct ggcaaccggt acagcgattg gcccttattt atcgattctg
    13441 caactaggca aagatttaga tcgcttcaaa aatctggtcc tggttcacgc cgcacgttat
    13501 gccgccgact taagctattt gccactgatg caagaactgg aaaaacgcta cgaagggaaa
    13561 ctgcgcattc agacggtggt cagtcgggaa acggcagcgg ggtcgctcac cgggcggata
    13621 ccggcgttaa ttgaaagtgg ggaactggaa agcgcgattg gcctgccgat gaataaagaa
    13681 accagccatg tgatgctgtg cggcaatcca cagatggtgc gcgatacaca acagttgctg
    13741 aaagagaccc ggcagatgac gaaacattta cgtcgccgac cgggccatat gacagcggag
    13801 cattactggt aagcggttac ttatcgataa acggcacgat gagcaaatcc gcactcatct
    13861 tattgatcat cccgcgatat gccggcatca aacggttgat aaatgagtga tgatgaccac
    13921 agacaaggag gtcgcactgc tctttttgca taatttccag cagtgtttcc ggcatttctc
    13981 cgcgttcaat acgcagtttt gtcttcggcc attgaatatt tttcgtcagt ttatacagct
    14041 tgttatccga cttattcttc aacaattgaa gaatatcttc tgttgcaggg aagtagatac
    14101 ccggatacaa ctcgcttaag ccatcatcaa tatgaattaa cgtcaggtga gcgtcattat
    14161 gtctggcgag ctccagagct ttattcacca gtaaggcatc ttcttcattc ccggaaattg
    14221 ccacgccaat gtgtttataa gccatgttta actccttcta aagcaactcc atcaagctaa
    14281 cgaacgcagt gataactcaa aaataatcat ctcggcctgg catgagaaag tgaaggccgc
    14341 atcaagctca acttgcccct ccagatcctt aatcttgtag gccacgctgg ccccttcatc
    14401 tgcagaggat gcagcggcgg caagctcttt cagtttggtc ataaaagact ctgcttcttc
    14461 gcgggtggca aaaaagcgcg aaaacttact ggtacagttg tcgttatcaa tcaccgtacc
    14521 gatatcaatc gcacatcctt tagtactgca tttatctacg acatctttca tagggcacct
    14581 ctgtatattc gcctctctgt cgattacgac ttaagaataa tgcgccgtta agggaagaac
    14641 aaacaagtaa tgatcttatg gattaccatt ttttttgata ttgcttaagg tgaagagttc
    14701 agaaggattt tccgggcatt gtcatttgca tcgtggaaat aacaatcccg gaaaaaaata
    14761 gcagtaagag atatattaaa ggttaattac ttgatttatt gtcggcttta tacttcacat
    14821 cctgggtatc tttaccatat ttattttcgc cttgtgtgcc aatgaacgct ccaagatcga
    14881 taagcatcat caccagaatc aacgtcggga caaaacgccc caccgcccat tgccagacac
    14941 ccggtaaaat cgcccagtta cccgccagca gcatccacgc cacaatcatc agaaatgccc
    15001 atgcgccgga acgcccgcga tcatgcaagc gcttaacggt tactgccgct gttggccaaa
    15061 gcaagcacac aaggcaaaac gccgcggtct gaatatcgag taaattttta cccgccagtg
    15121 agaaaagcac cagcatgcct gcgaaccaca ggcctatcca aatccagaaa tcacggcgtc
    15181 caatacgccc tttaaatgag aataaccatt gctgtatggt catgtaagtt ccttgatggt
    15241 tgtcttttcc aggattctac ccttttgaca agggcgacag atatcgtttt aatcggagcc
    15301 agtcataaca aaaggtactg tcaatgaagc cagggtgtac gctgtttttt ctcttatgtt
    15361 ctgcattaac cgttacaaca acggcgcatg cacaaacacc agatacggca acgaccgcgc
    15421 cttatctgct ggctggagcc cctactttcg atctctccat cagccagttt cgagaagact
    15481 ttaacagcca gaatcccagc ctgccactga acgaatttcg tgccatcgac agcagtcccg
    15541 acaaagccaa tctcactcgt gctgcaagta aaattaacga gaacttgtat gcttctacag
    15601 cgctggagcg cggtacctta aaaatcaaaa gcattcaaat gacctggcta cccatccagg
    15661 ggccagagca aaaagcagcg aaagcgaaag ctcaggaata catggcagca gtgatccgca
    15721 cactcacccc attaatgacc aaaacacaaa gccagaaaaa actgcagtcg ctgctaacgg
    15781 cggggaaaaa caaacgttat tacaccgaga cagaaggtgc actgcgttat gttgtcgcgg
    15841 acaacggcga aaaggggctg accttcgctg ttgaaccgat taagctggcg ctatctgaat
    15901 cgcttgaagg tttgaataaa tgacaaaaag caaagccttt gtgccgatga atctctatac
    15961 tgtttcacag acctgctgcc ctgcggggcg gccatcttcc tttattcgct tataagcgtg
    16021 gagaattaaa atgcgacatc ctttagtgat gggtaactgg aaactgaacg gcagccgcca
    16081 catggttcac gagctggttt ctaacctgcg taaagagctg gcaggtgttg ctggctgtgc
    16141 ggttgcaatc gcaccaccgg aaatgtacat cgatatggcg aagcgcgaag ctgaaggcag
    16201 ccacatcatg ctgggtgcgc aaaacgtgga cctgaacctg tccggcgcat tcaccggtga
    16261 aacttctgcc gctatgctga aagacatcgg cgctcagtac atcatcatcg gccactctga
    16321 acgtcgtact taccacaaag agtctgacga actgatcgcg aaaaaattcg cggtgctgaa
    16381 agagcagggc ctgactccgg ttctgtgcat cggtgaaacc gaagctgaaa acgaagcggg
    16441 caaaactgaa gaagtttgcg cacgtcagat cgacgcggta ctgaaaactc agggtgctgc
    16501 ggcattcgaa ggtgcggtta tcgcttacga acctgtatgg gcaatcggta ctggcaaatc
    16561 tgcaactccg gctcaggcac aggctgttca caaattcatc cgtgaccaca tcgctaaagt
    16621 tgacgctaac atcgctgaac aagtgatcat tcagtacggc ggctctgtaa acgcgtctaa
    16681 cgctgcagaa ctgtttgctc agccagatat cgacggcgcg ctggttggcg gtgcttctct
    16741 gaaagctgac gctttcgcag taatcgttaa agctgcagaa gcggctaaac aggcttaagt
    16801 ctgacaggtg ccggatttca tatccggcac ttactttcct taactcttcg ccttaacgca
    16861 aaatctcaca ctgatgatcc tgaatttcct cggctgaagc acggttaagc gtcagtagat
    16921 ttcgttgtgt cgccagcaat acaaatgagt tatcactctg ccgtaccatc gccagcccgt
    16981 agcttcccat atgttcccgc gcctcaggta cttcttctgc cagcatcata aatgggctgc
    17041 gttgtaccag ttcgctttcc gttacccgac gcgccaggta ttcatgcccg cgcaaaccac
    17101 ctggcagtgg caaccagcgg ctgctgatgt tcgccagatt gttatccagt tgttcgcgca
    17161 catcaggacg aatacaagag atatgaatat gaaaatggtt ttgcgtacgc ccggtgcggg
    17221 agttgatcgc caaagaaacc gcgcgatcgg gaaccggctg gccgtatttt ttgctcataa
    17281 aatcacgcgc ctgccaggcc aaccaaaaga agttcggcgt tgaaggatcg gtcaacaaag
    17341 gactttcagt accgttaata cgatacgttg gcatcaacag atattgcagt ggaccattaa
    17401 gatcttttaa aaccacgtat ccggcattgg gtttgacttc cgcacatggc gaaggatttt
    17461 gattttgctg ctgattgggc aaacattcct caaggacaat cttacgtaat gtatccgact
    17521 cttcaccggt taatttccag taaccaatac cggcagccac aacggcgata actatcatca
    17581 ccaaaaaaag aagacccgct tttttcatct ttttttccct gtacctcaaa gagatgcaag
    17641 ggtaacgcaa aatcgtgaca aataaaaaac ccggtcacga gaaaccaccg ggtttgataa
    17701 ttatcctggg gaaatcagcg tttgctgatc tgatcgaacg taccgccgtt agcaaaatgc
    17761 tctttttgcg ctttcgtcca gccgccgaac tcttcatcaa tggtgaataa cttcagcttt
    17821 ggaaacgcat tttcgtactt tttcgccacc tcagcgtcgc gcggacggta gtagtttttc
    17881 gcggcaattt cctgaccttc tggcgagtag agatatttca ggtaggcttc cgccacctct
    17941 ttggtgcctt ttttctcgac cactttatcg accaccgaca cggttggctc tgcgaggata
    18001 gactcactcg gcgtgacgat ttcgaattta tctttcccca gttcattcgc tgccagcaga
    18061 gcttcgtttt cccaggcgat cagtacatcg ccaatcccgc gctcgacaaa ggtgttagtg
    18121 gagccgcgcg cgccagaatc cagaacttcg acgtttttat acagtgcccg aacaaaatcc
    18181 tgtgcttttg cctgatcgtt gttgttgtga tgcagcgcgt agccccaggc agccaggtag
    18241 ttccagcgtg cgccaccaga gcttttcgga ttaggcgtga tcaccgaaac acccggttta
    18301 atcagatcgt tccagtcatg gatctgcttc ggattgccct tacgcaccag gaaaacaatg
    18361 gtggaagtgt acggtgcgga gttatccggc agacgtttga tccactcttt atcaatccgc
    18421 ccgcgttccg caattgcgtc cacgtcatag gccagagcca gcgtgacaac atcagcttca
    18481 ataccgttga ttaccgacgt cgcttgttta cctgaaccac cgtgtgactg acgaatcacc
    18541 acgttatcac cagtttgctg tttccagtgg gcgctgaatg ccttgttgta ctgttcgtac
    18601 aattcgcgcg ttggatcata tgaaacgtta agaagctgaa tatcctttgc cataacgctg
    18661 gttgccgcca gcaaaaatgt taaccctacg ccccacttgt tcatcgcccg actctcttat
    18721 gttgtgttgt gatgagcaaa gcgtgccaga aggttaacca aacattaaag aataaaaaaa
    18781 gattggctat aacttgtggg tatatgttga gggattaaaa aggcggaaga aatccgcctc
    18841 atattgctga caaagtgcgc tttgtccatg ccggatgcgg cgcgaacgcc ttatccggcc
    18901 tacaaaagtt tgcaaattca ataaattgca gaattcatgt aggcctgata agcgaagcgc
    18961 atcaggcatt tttgcttctg tcatcggttt cagggtaaag gaatctgcct ttttccgaaa
    19021 tcattaatac agttttttcg cgcagtccag ccagtcacct ttgaacggac gcttcatgtt
    19081 ttcgatagcg tcgatgatgt cgtggtgaac cagctgttcg ttctggatac ctacacaacg
    19141 accgccgtaa cctgccagca gcagatcgat agcgtaagcg cccatacggg aagccagaat
    19201 acggtcgtaa ggcaccggag aaccaccgcg ctggatgtgg cccagcacag ttgcgcgggt
    19261 ttcacgaccg gtttctttct cgatgaaatg cgccagttcg tcaacatcac acatatgttc
    19321 ggtaatcgcc acgatcgcgt gttttttacc tttcgcgata cccgctttga tttcgtttac
    19381 caggtcttca cggctgaatt caacttccgg aaccacaacg aattcacagc caccggcaat
    19441 ggccgcagcc aacgtcagat cgccacaata acggcccatc acttccacca cggaaatacg
    19501 ctggtgagaa gaagaggtgt cacgcagacg gtcgatcgct tctacaacgg tgctcagcgc
    19561 agtgaagaaa ccgatagtgt agtcagtgcc tttgatgtcg ttgtcgatag tgcccggcag
    19621 accgatgcac gggaagccca tttcggtcag acgcattgca cccatgtagg aaccgtcacc
    19681 gccgataacc accagcgcgt cgatcccacg ttttttcagg ttttcgatag ccacggcgcg
    19741 gatgttctcg tcgcggaatt ccgggaagcg cgcagaaccg aggaacgtac cgccacggtt
    19801 gatcatgtca gaaacgctgt aacggtctag ctgtaccata cggtcttcat acagacccag
    19861 atagccgtca taaatgccca ttacttccag accttctgtc agcgcagaac gaacaacccc
    19921 gcgaattgcg gcgttcatgc ctggcgcatc accgccgctt gtcaacacac cgattttctt
    19981 aatcatgact acctctgaac tttggaatgc aaaataaaat ctgttgccgg aagtcttctt
    20041 gcacatcgaa gtgatccaac gaatgtgcaa atagtataac aatcacttcc tgctgaattg
    20101 attcaggtca ggccaaatgg cggtatttta tacacaaaat gcgggtctgg ctctctttta
    20161 tactgattat gaaagcatag accgtttacc ctccctgggt acgacggaac aggggtcctg
    20221 atggataatt acatccgatc ccggaaaacg ccgtaaaata gcctgctcta cctgatccgc
    20281 caccatatgt gcctgaacca aaggcagaga gtcttccatt tccaaatgaa tctgaataaa
    20341 gcgggtcggc cctgactgcc gcgtgcgaag atcgtgagcg ccgctaacac ccggccagga
    20401 agtcacgata tcaataattt cttgccgttc ctcatcaggc aatgcgcgat ccagtaatga
    20461 ctgtaccgcc tcatatccca tgcgtaacgc gctatataaa atatagatgc cgattcccaa
    20521 tgcaaacaga gcatcggcgc gatgccagcc gtaccaggac aaccccagcg ccagcagaat
    20581 tgcgccgttc atcataacat cagactggta atgtagcata tcagcccgca ccgcctggct
    20641 ttgcgtccgg cgcaccaccc aacgctgaaa cgagacaagg ataatcgtac aaattagcgc
    20701 cacaattgtc acgataaccc cgacgcctgg atctgtcatc ggtgttggag atatcagatg
    20761 ttgaataccc gtcaaaaaca ggaatagtgc cgaaccggag ataaacatac tttgcgccag
    20821 cgccgcgagg gactctgctt taccgtgacc aaacgagtga ttatcgtcgg caggttgcag
    20881 ggaatatcgc accaccagta aattcgtcaa cgacgcgccg atatccacca gcgaatccac
    20941 cagcgcggcg agaatactca ccgacccggt ataccaccat gcaaaaattt taatcagcaa
    21001 tagcaacgaa gccatcgccg tcgcagcaat cgccgcccga ctgaccagcc gtccataaga
    21061 ttgattcata aatactcccg ctatcaactg acgctagtat aacggaagca aatcatctgc
    21121 aatgcattaa gcagcaggca aattgaggat aaaaaaaacc cccacatcat gtgggggaag
    21181 acagggatgg tgtctatggc aaggaaaaca gggtttacta ctgggaacgt gagttgctac
    21241 tactcaatag cttcaacgat gaactttttt gccattgcgt cacgtcacgc aactgctcca
    21301 ttcgttgttg atgtttctcg tttaaaaccg cttgctgctc cggcgttaac aggcgataca
    21361 tttggttgcg gacttttgcc atctcaacct gacgagcaat ttgctcattc gccatttttt
    21421 ctgcctgtgc gcgcacagcg ttttcatcaa aattttctgc ggtgacaagg cgatgcattg
    21481 tctccagttc gctaacatta acaggaggct gttcgtgccg ggcctgttgc ataagatctc
    21541 gcatctgctg acgctgatgt tcggttaaac ttatgccgtc gaacatatgg ctctgcgtac
    21601 tgcgctgcgt aagttcttca cccggatgcc agttatcgcc tgaaccgact tcagcagcgt
    21661 ggcttaatga actgactgcc agcgttgagg ccatgacggc agcggtaact atgcgcatca
    21721 tttgctccca aaatctttct gtcgcgattc aacgatagag agtttacgat tcaggctgca
    21781 aacatgcgtc agggggtgta aaacaacgta aagtcatgga ttagcgacgt ctgatgacgt
    21841 aatttctgcc tcggaggtat ttaaacaatg aataaaatcc tgttagttga tgatgaccga
    21901 gagctgactt ccctattaaa ggagctgctc gagatggaag gcttcaacgt gattgttgcc
    21961 cacgatgggg aacaggcgct tgatcttctg gacgacagca ttgatttact tttgcttgac
    22021 gtaatgatgc cgaagaaaaa tggtatcgac acattaaaag cacttcgcca gacacaccag
    22081 acgcctgtca ttatgttgac ggcgcgcggc agtgaacttg atcgcgttct cggccttgag
    22141 ctgggcgcag atgactatct cccgaaacca tttaatgatc gtgagctggt ggcacgtatt
    22201 cgcgcgatcc tgcgccgttc gcactggagc gagcaacagc aaaacaacga caacggttca
    22261 ccgacactgg aagttgatgc cttagtgctg aatccaggcc gtcaggaagc cagcttcgac
    22321 gggcaaacgc tggagttaac cggcactgag tttaccctgc tctatttgct ggcacagcat
    22381 ctgggtcagg tggtttcccg tgaacattta agccaggaag tgctgggcaa acgcctgacg
    22441 cctttcgacc gcgctatcga tatgcacatt tccaacctgc gtcgtaaact gccggatcgt
    22501 aaagatggtc acccgtggtt taaaaccttg cgtggtcgcg gctatctgat ggtttctgct
    22561 tcatgatagg cagcttaacc gcgcgcatct tcgccatctt ctggctgacg ctggcgctgg
    22621 tgttgatgtt ggttttgatg ttacccaagc tcgattcacg ccagatgacc gagcttctgg
    22681 atagcgaaca gcgtcagggg ctgatgattg agcagcatgt tgaagcggaa ctggcgaacg
    22741 atccgcccaa cgatttaatg tggtggcgac gtctgttccg ggcgattgat aagtgggcac
    22801 cgccaggaca gcgtttgtta ttggtgacca ccgaaggccg cgtgatcggc gctgaacgca
    22861 gcgaaatgca gatcattcgt aactttattg gtcaggccga taacgccgat catccgcaga
    22921 agaaaaagta tggccgcgtg gaactggtcg gtccgttctc cgtgcgtgat ggcgaagata
    22981 attaccaact ttatctgatt cgtccggcca gcagttctca atccgatttc attaacttac
    23041 tgtttgaccg cccgctatta ctgctgattg tcaccatgtt ggtcagtacg ccgctgctgt
    23101 tgtggttggc ctggagtctg gcaaaaccgg cgcgtaagct gaaaaacgct gccgatgaag
    23161 ttgcccaggg aaacttacgc cagcacccgg aacttgaagc ggggccacag gaattccttg
    23221 ccgctggtgc cagttttaac cagatggtca ccgcgctgga gcgcatgatg acctctcagc
    23281 agcgtctgct ttctgatatc tctcacgagc tgcgcacccc gctgacgcgt ctgcaactgg
    23341 gtacggcgtt actgcgccgt cgtagtggtg aaagcaagga actggagcgt attgaaaccg
    23401 aagcgcaacg tctggacagc atgatcaacg atctgttggt gatgtcccgt aatcagcaaa
    23461 aaaacgcgct ggttagcgaa accatcaaag ccaaccagtt gtggagtgaa gtgctggata
    23521 acgcggcgtt cgaagccgag cagatgggca agtcgttgac ggttaacttc ccgcctgggc
    23581 cgtggccgct gtacggcaat ccgaacgccc tggaaagtgc gctggaaaat attgttcgta
    23641 atgctctgcg ttattcccat acgaagattg aagtgggctt tgcggtagat aaagacggta
    23701 tcaccattac ggtggacgac gatggtcctg gcgttagccc ggaagatcgc gaacagattt
    23761 tccgtccgtt ctatcggacc gatgaagcac gcgatcgtga atctggcggt acaggattgg
    23821 ggctggcgat tgttgaaacc gccattcagc agcatcgtgg ctgggtgaag gcagaagaca
    23881 gcccgctggg cggtttacgg ctggtgattt ggttgccgct gtataagcgg agttaaactc
    23941 cgcatttgta ggcaggataa ggcgtttacg ccgcatccgg catttgagca ggatgcctga
    24001 tgcgacgctg atagcgtctt atcaggccta cactccgggg gttattttcc ccacaaccgg
    24061 cgggaaaaat cttcgatctt gccgctcagt cggcgctttt gcatcgtccg cgtccagctt
    24121 ttcgataacc cggcggcgga gagtaaacgg tgatattgat cgtcatcaaa cggcatatgc
    24181 catgcgatgg cggcagcctc ctgcacggtc acatcgctga cacgggaaac caactccagc
    24241 ggcgcgtccg cagacacttt ccctggcgct atcacgctat acaaccaccc caccttacct
    24301 gtgttttgca tcaactgcgc tatatcgctg atatcaaaat ggtaattgag tttatagcaa
    24361 ggcgagcgcg gctggctgac ctgaattaat gcctctcccc agcggaaaat atcgcccata
    24421 taaacattac tttccgtcag gccgtcggtt gagaggtttt caccaaacgc aggcgcaaca
    24481 aacaactccg cctgttccgg aaattcccgc gcccagtaga gataatgctc acgaggataa
    24541 tgacacagcg ctctgtctgg cccgccgtga acctttttct ccgcctgctc gtcaccttcc
    24601 agccccagct ccgtcagcat caactcacca tcaacctgga ttttggcaat tgcgctgggt
    24661 ttgccttcgg gataagcctg aatcttgcct gtgtatacat caaccggata tcgcatctgt
    24721 tccctctcca cgagaaattt ccccattaga ccatatttat tttatgtcag gcgtgctgtt
    24781 ttcgcggtaa gtatccaggc aggaactcaa tagcaaaccg gacgaacgtt agttcatccg
    24841 gctgtgggga ttaaggaagc aggtttattt caccgtacct aaaatttcga ttttcgctgc
    24901 tctggctttg actttacgtg accagataga ggtaagaatt ggcaccagaa tcgaagtcac
    24961 aatgaccgcc gtcgctacca gcgaagttgc tgccggagcc atcggtttaa acgcaggcac
    25021 catttctgca atcagcacag gtgtcgctac cgcggcccct gcggaactgg aagcggcaat
    25081 tccggcagtg ccatcgccac cgccaatcaa tttatcggcg ataatcaacg gaataccggt
    25141 cacgataatt actgccacac ccaacaggat ccccagcaaa ccagtctggg caatcacagt
    25201 caaatcaatg gtattgccca gcgcgaaggc aaagaatgga atcagcgttt gcaccgcttt
    25261 gctgaaaaat tctcgtaatt cagggtcaag gttccccagg gcaaagccca ccaggaacgg
    25321 taatactgcg ccgacgaaaa catgcggttc aaacgaggca atccctgcag tgcccagaat
    25381 aatcatcgtc atgagcggcc cggactccaa cgacatcaac acaaatgccc cagcttcttc
    25441 ttttgtgccg tactgctgca tgatggaagc gtaaagtccg ccattggtca tatccatcgc
    25501 cgccaccagc gccagcgttg aaagtccggc aaagaatcca acttcaacac catgttccgg
    25561 aataatgcgc gaggcaattg ccgcaaccac ccacgcgacg gcaatttttg ttaccaccag
    25621 cgtaccggat ttacgcaata ccgttcccgt cgcgcttaat tttattgacg cccccatgca
    25681 aaaaaaccac accgccagaa tgggcaccgt accggtaatc atcccgttgg tgaatgatcc
    25741 aaaatatttc cccgcccccg gcgagaaggt gtggcacagt gcgccaagga atagcgggac
    25801 gagcatcatc ccccccggga ttttctcaat cgagcgtttt atctgcattt ccatcacctg
    25861 tcggcatatt tttaagggct gatgcgtcat tcctcaatgg agcatcagtg ttgatggaaa
    25921 cgaagataga cattcattta atcaataaaa gtgatcaaaa ccacatcaat tgaaacactg
    25981 ttttattttt atcggatcat tgtttttatt ttgaaattga tcacaaaaaa accaccgccg
    26041 ttggcgatgg ttcatttttt aagctgatat gcggcctata cgcctcattg cagcaggcgg
    26101 caaatgatta ttttttcgcc gcgaaacgcg ccgcagcttc gtcccagttc accacgttcc
    26161 agaactcttt gatgtagtcc gggcggcggt tctggaattt caggtagtaa gcatgttccc
    26221 acacatccag gcccagaatc gggaagccgg aagcgccaga aatagcttca cccatcagcg
    26281 gagaatcctg gttagcagta gaaaccaccg ccagtttatc gcctttcagc accagccatg
    26341 cccagccgga accaaagcgg gaagctgccg ctttttcaaa ttctgctttg aagttatcaa
    26401 cggagccgaa gtcacgttcg atagccgctt tcaggtcacc ctgcagggtg gtgccttttt
    26461 tcagaccttt ccagaacagg ctgtggttag cgtgaccgcc agcgttgttg cgcagtacgg
    26521 ttttcttgtc tgctggcagc tggtccagtt tggtgatcag ctcttcaacc ggcaggttgg
    26581 caaattctgg caggctttcc agcgccgcgt tggcgttgtt tacgtaggtc tgatggtgtt
    26641 tggtgtggtg gatttccatg gtctgcttat cgaagtgcgg ttccagggca tcgtaagcat
    26701 acggcaggga tggcagggta tagctcatat tcatctccag tattgtcggg cggccgattg
    26761 ttaatgccgc gtaagcagtt ggttcattat agttaattaa atgatattga aaatgattat
    26821 caatgccgta cttttcgtaa gggtatggtt ttgcaggaaa atgcccgaga tgtgaagcaa
    26881 atcacccact taatgccgtg attgccagta aatcgacaac ggcggcaaca ggcgaaaggt
    26941 taatcgacag cacgattttt acactcatct cgtcggagat gtgaagcgac gaaaaatgat
    27001 gaggataaga agatgagtaa cgcgattacg atggggatat tttggcattt gatcggcgcg
    27061 gccagtgcag cctgttttta cgctccgttc aaaaaagtaa aaaaatggtc atgggaaacc
    27121 atgtggtcag tcggtgggat tgtttcgtgg attattctgc cgtgggccat cagcgccctg
    27181 ttactaccgg atttctgggc gtattacagc tcgtttagtc tctctacgct gctgcctgtt
    27241 tttctgttcg gcgctatgtg ggggatcggt aatatcaact atggcctgac catgcgttat
    27301 ctcggcctgt cgatgggaat tggcatcgcc attggcatta cgttgattgt cggtacgctg
    27361 atgacgccaa ttatcaacgg caatttcgat gtgctgatta acaccgaagg cggacgcatg
    27421 acgttgctcg gcgttctggt ggcgctgatt ggcgtaggga ttgtgactcg cgccgggcag
    27481 ttgaaagagc gcaaaatggg cattaaagcc gaagagttca acctgaaaaa agggctggtg
    27541 ctggcggtga tgtgcggcat tttctctgcc ggtatgtctt ttgcgatgaa cgccgcaaaa
    27601 ccgatgcatg aagccgctgc cgcactgggc gtcgatccac tgtatgtcgc tctgccaagc
    27661 tatgtcatca tcatgggcgg cggcgcgatc atcaacctcg gtttctgctt cattcgtctg
    27721 gcaaaagtga aggatttgtc gctaaaagcc gacttctcgc tggcaaaacc gctgatcatt
    27781 cacaatgtgt tactctcagc actgggcggg ttgatgtggt atctgcaatt ctttttctat
    27841 gcctggggcc acgcccgcat tccggcgcag tatgactaca tcagttggat gctgcatatg
    27901 agtttctatg tattgtgcgg cggtatcgtc gggctggtgc tgaaagagtg gaacaatgca
    27961 ggtcgccgtc cggtaacggt gttgagcctc ggttgtgtgg tgattattgt cgccgccaac
    28021 atcgtcggca tcggcatggc gaattaatct ttctgcgaat tgagatgacg ccactggctg
    28081 ggcgtcatcc cggtttcccg ggtaaacacc accgaaaaat agttactatc ttcaaagcca
    28141 cattcggtcg aaatatcact gattaacagg cggctatgct ggagaagata ttgcgcatga
    28201 cacactctga cctgccgcag atattgattg atggtcattc cagtctgctg gcgaaattgc
    28261 tgacgcaaaa cgcgctcact gcacgatgcc tcatcacaaa atttatccag cgcaaaggga
    28321 cttttcaggc tagccgccag ccgggtaatc agcttatcca gcaacgtttc gctggatgtt
    28381 ggcggcaacg aatcactggt gtaacgatgg cgattcagca acatcaccaa ctgcccgaac
    28441 agcaactcag ccatttcgtt agcaaacgac acatgctgac tactttcatg ctcaagctga
    28501 ccgataacct gccgcgcctg cgccatcccc acgctaccta agcgccagtg tggttgccct
    28561 gcgctggcgc taaatcccgg aatcgctccc tgccagtcaa gattcagctt cagccgctcc
    28621 gggcaataaa taatattctg caaaaccaga tcgttaacgg aagcgtagga gtgtttatcg
    28681 tcagcatgaa tgtaaaagag atcgccacgg gtaatgcgat aagggcgatc gttgagtaca
    28741 tgcagaccat taccgcgcca gacaatcacc agctcacaaa aatcatgtgt atgttcggca
    28801 aaaacatctt gcggataacg gtcagccaca gcgactgcct gctggtcgct ggcaaaaaaa
    28861 tcatctttga gaagttttaa ctgatgcgcc accgtggcta cctcggccag agaacgaagt
    28921 tgattattcg caatatggcg tacaaatacg ttgagaagat tcgcgttatt gcagaaaacc
    28981 atcccgtccc tgacgaatat cacgcggtga ccagttaaac tctcggcgaa aaagcgtcga
    29041 aaagtggtta ctgtcgctga atccacagcg ataggcgata tcagtaacgc tggcctcgct
    29101 gtggcgtagt agatgtcggg ctttcatcag tcgcaggcgg ttcaggtaac gctgaggcgt
    29161 cagtcccgtt tgctgcttaa gctgccgatg tagcgtacgc agcgaaagag aaaattgctc
    29221 cgccacggca tcccaattca cctcatcggc aaaatggtcc tccagccagg ccagaagcaa
    29281 gttgagacgt gatgcgctgt tttccaggtt ctcctgcaaa ctgcttttac gcagcaagag
    29341 cagtaattgc ataaacaaga tctcgcgact ggcggtcgag ggtaaatcat tttccccttc
    29401 ctgctgttcc atctgtgcaa ccagctgtcg cacctgctgc aatacgctgt ggttaacgcg
    29461 ccagtgggaa ggatactgcc catccagctc ttgtggcagc aactgattca gcccggcgag
    29521 aaactgaaat cgatccggcg agcgatacag cacattagtc agacacagat tatcggtatg
    29581 ttcatacaga tgccgatcat ggtcgcgcac aaaacagacc gtgccaccgg tgatggtata
    29641 gggctgccca ttaaacacat gaatacccgt gccatgttcg acaatcacaa tttcatgaaa
    29701 atcatgatga tgttcaggaa aatccgcctg cgggagccgg ggttctatcg ccacggacgc
    29761 gttaccagac ggaaaaaaat ccacactatg taatacggtc atactggcct cctgatgtcg
    29821 tcaacacggc gaaatagtaa tcacgaggtc aggttcttac cttaaatttt cgacggaaaa
    29881 ccacgtaaaa aacgtcgatt tttcaagata cagcgtgaat tttcaggaaa tgcggtgagc
    29941 atcacatcac cacaattcag caaattgtga acatcatcac gttcatcttt ccctggttgc
    30001 caatggccca ttttcctgtc agtaacgaga aggtcgcgaa ttcaggcgct ttttagactg
    30061 gtcgtaatga aattcagcag gatcacatta tgacctttcg caattgtgtc gccgtcgatc
    30121 tcggcgcatc cagtgggcgc gtgatgctgg cgcgttacga gcgtgaatgc cgcagcctga
    30181 cgctgcgcga aatccatcgt tttaacaatg ggctgcatag tcagaacggc tatgtcacct
    30241 gggatgtgga tagcctggaa agtgccattc gccttggatt aaacaaggtg tgcgaggaag
    30301 ggattcgtat cgatagcatt gggattgata cctggggcgt ggactttgtg ctgctcgacc
    30361 aacagggtca gcgtgtgggc ctgcccgttg cttatcgcga tagccgcacc aatggcctga
    30421 tggcgcaggc acaacaacaa ctcggcaaac gcgatattta tcaacgtagc ggcatccagt
    30481 ttctgccctt caatacgctt tatcagttac gggcgctgac ggagcaacaa cctgaactta
    30541 ttccacacat tgctcacgct ctgctaatgc cggattattt tagttatcgc cttaccggca
    30601 agatgaactg ggagtacacc aacgccacaa ccacgcaact ggtcaatatc aatagcgacg
    30661 actgggacga gtcgctactg gcgtggagcg gtgccaacaa agcctggttt ggtcgcccga
    30721 cgcatccggg taatgtcata ggtcactgga tttgcccgca gggtaatgag attccggtgg
    30781 tcgccgttgc cagccatgat accgccagcg cggttatcgc ctcgccgtta aacggttcac
    30841 gcgccgctta tctctcttct ggtacctggt cattgatggg cttcgaaagc cagacgccat
    30901 ttaccaatga cacggcgctg gcagccaaca tcaccaatga aggcggggcg gaaggtcgct
    30961 atcgggtgct gaaaaatatt atgggcttat ggctgcttca gcgggtgctt caggaacggc
    31021 aaatcaacga tctcccggcg cttatcgccg cgacacaggc acttccggcc tgtcgcttca
    31081 tcatcaatcc caatgacgat cgctttatta atcctgaagc gatgtgcagc gaaattcagg
    31141 ctgcgtgtcg ggaaacggcg caaccgatcc cggaaagtga tgctgaactg gcgcgctgta
    31201 ttttcgacag tctggcgctg ctgtatgccg atgtgttgca tgagctggcg cagctgcgcg
    31261 gtgaagattt ctcgcaactg catattgtcg gcggcggctg ccagaacacg ctgctcaacc
    31321 agctatgcgc cgatgcctgc ggtattcggg tgatcgccgg gcctgttgaa gcctcaacgc
    31381 tcggcaatat cggcatccag ttaatgacgc tggatgaact caacaatgtg gatgatttcc
    31441 gtcaggtcgt cagcaccacc gcgaatctga ccacctttac ccctaatcct gacagtgaaa
    31501 ttgcccacta tgtggcgcag attcactcta cacgacagac aaaggagctt tgcgcatgac
    31561 cactcaactg gaacaggcct gggagctggc gaaacagcgt ttcgcggcgg tggggattga
    31621 tgtcgaggag gcgctgcgcc aacttgatcg tttacccgtt tcaatgcact gctggcaggg
    31681 cgatgatgtt tccggttttg aaaacccgga aggttcgctg accggtggga ttcaagctac
    31741 tggtaattat ccgggcaaag cgcgtaatgc cagtgagcta cgtaccgatc tggaacaggc
    31801 tatgcggctg attccggggc cgaaacggct taatttacat gccatctatc tggaatcaga
    31861 tacgccagtc tcgcgcgatc agatcaaacc agagcacttc aaaaactggg ttgaatgggc
    31921 gaaagccaat cagctcggtc tggattttaa cccctcctgc ttttcgcatc cgctaagcgc
    31981 cgatggcttt acgctttccc atgccgacga cagcattcgc cagttctgga ttgatcactg
    32041 caaagccagc cgtcgcgttt cggcctattt tggcgagcaa ctcggcacac catcggtgat
    32101 gaacatctgg atcccggatg gtatgaaaga tatcaccgtt gaccgtctcg ccccgcgtca
    32161 gcgtctgctg gcagcactgg atgaggtgat cagcgagaag ctaaaccctg cgcaccatat
    32221 cgacgccgtt gagagcaaat tgtttggcat tggcgcagag agctacacgg ttggctccaa
    32281 tgagttttac atggggtatg ccaccagccg ccagactgcg ctgtgcctgg acgccgggca
    32341 cttccacccg actgaagtga tttccgacaa gatttccgcc gccatgctgt atgtgccgca
    32401 gttgctgctg cacgtcagcc gtccggttcg ctgggacagc gatcacgtag tgctgctgga
    32461 tgatgaaacc caggcaattg ccagtgagat tgtgcgtcac gatctgtttg accgggtgca
    32521 tatcggcctt gacttcttcg atgcctctat caaccgcatt gccgcgtggg tcattggtac
    32581 acgcaatatg aaaaaagccc tgctgcgtgc gttgctggaa cctaccgctg agctgcgcaa
    32641 gctggaagcg gcgggcgatt acactgcgcg tctggcactg ctggaagagc agaaatcgtt
    32701 gccgtggcag gcggtctggg aaatgtattg ccaacgtcac gatacgccag caggtagcga
    32761 atggctggag agcgtgcggg cttatgagaa agcgattttg agccagcgtg ggtaaacact
    32821 gccggatgcg gcgcgagcgc cttatccggc ctacaggtcg aatgtccatg cgggctgtca
    32881 ccctcacccc ggccctctcc ctgaaagggc gagggagaaa aacatagcct cgtgcggcaa
    32941 cggagctgaa cactgctccg gacagtcccc tcgcccctac ggggagaggg ttagggtgaa
    33001 gggaacaagc agcaccatgt ctaaacaagg caacctaatt tacaggaaca cagaacatgc
    33061 aaaacattac ccagtcctgg tttgtccagg gaatgatcaa agccaccacc gatgcctggc
    33121 tgaaaggctg ggacgagcgc aacggcggta acctgacgct gcgcctggat gacgccgata
    33181 tcgcgcctta taaagacaac ttccatgcac aaccgcgcta tatcccgctc agccaaccca
    33241 tgcctttact ggcaaataca ccgtttattg tcaccggctc cggcaaattc ttccgtaacg
    33301 tccagcttga tcctgcagct aacttaggcg tcgtaaaagt cgacagcgac ggcgcgggct
    33361 accatattct ctgggggtta accaacgaag ccgttccaac ttctgaactt ccggctcact
    33421 ttctctccca ctgcgagcgc attaaagcca ccaacggtaa agatcgggtg atcatgcact
    33481 gccacgccac caacctgatc gccctcacct atgtactgga aaacgacacc gcggtcttta
    33541 ctcgccaact gtgggaaggc agcaccgagt gtctggtagt gttcccggat ggcgttggca
    33601 ttttgccgtg gatggtgccc ggcacggacg aaatcggcca ggcgaccgca caagagatgc
    33661 aaaaacattc gctggtgttg tggcccttcc acggcgtctt cggcagcggg tcgacgttgg
    33721 atgaaacatt cggtttaatc gacaccgcag aaaaatcagc acaagtatta gtgaaggttt
    33781 attcgatggg cggcatgaaa cagaccatca gccgtgaaga gttgatagcg ctcggcaagc
    33841 gcttcggcgt tacaccacta gctagtgcgc tggcgctgta aggagcaaac atgatccgca
    33901 aagcctttgt catgcaggtt aaccccgacg cccacgaaga gtatcagcgt cggcataacc
    33961 ccatctggcc ggaactggaa gcagtgttga aatctcacgg tgcgcataac tacgccattt
    34021 acctcgacaa agcgcgtaat ctgctgtttg ccacggtaga gattgaatct gaagaacggt
    34081 ggaatgcggt agccagcact gatgtttgcc aacgttggtg gaaatatatg accgatgtta
    34141 tgcccgctaa cccggataac agcccggtga gtagcgagct gcaagaagtg ttttacctgc
    34201 cgtaattgcc caatgtgcca gagatgcctc cggcacaata ttttaaattt cgatttgaaa
    34261 aattaaacat att
//
LOCUS       DAZFXT010000045        32887 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.045, whole genome shotgun sequence.
ACCESSION   DAZFXT010000045 DAZFXT010000000
VERSION     DAZFXT010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32887
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.045"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..180)
                     /locus_tag="ACHKW0_002255"
     CDS             complement(<1..180)
                     /locus_tag="ACHKW0_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7475086.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGE"
     gene            1412..2896
                     /locus_tag="ACHKW0_002256"
     CDS             1412..2896
                     /locus_tag="ACHKW0_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO7475087.1"
                     /translation="MSFGQPCDEFPLSSLPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSDEAGIIFDGYTLSELPFINKMWDGSVLSVDRKNE
                     PEQMIENARMTLSLMVQPGLFDRYMERKGSVARDSGFLARCLISKPATTQGKRFINGA
                     VIPGGSLTAFHERLMELARGSIEKSSEDERYCLHFSPEAQKIFIEHYNVLEQDLSPSG
                     PLSPFRGHVSKKTENIARIAALFQYFSYGEGKISADIMTSAVVISSWYTDEYKKLFAL
                     PDESELQQKDAEELFDWLIEECRGECPPRVRKNYILQCGPGRFRNRKKLNALLNILES
                     QFRLSVVPEGKTMYVLLPQIASLKLSDVSGIFTSGYHYNKLRAK"
     gene            complement(3218..3820)
                     /locus_tag="ACHKW0_002257"
     CDS             complement(3218..3820)
                     /locus_tag="ACHKW0_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475088.1"
                     /translation="MKPQYQTRYELLHESYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRTTPPLDDDLIVNLCTSEHLLVHHPMLEG
                     ILLSECERLRQRSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNCLDDWTENLKRK
                     SEKELEEWIINRQKQSAALTDLMDQYVLLAYRTTVDDKRT"
     gene            complement(3907..4113)
                     /locus_tag="ACHKW0_002258"
     CDS             complement(3907..4113)
                     /locus_tag="ACHKW0_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7475089.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            4872..5021
                     /locus_tag="ACHKW0_002259"
     CDS             4872..5021
                     /locus_tag="ACHKW0_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha/YmoA family nucleoid-associated regulatory
                     protein"
                     /protein_id="HFO7475090.1"
                     /translation="MEKVISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
     gene            complement(5436..5636)
                     /locus_tag="ACHKW0_002260"
     CDS             complement(5436..5636)
                     /locus_tag="ACHKW0_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475091.1"
                     /translation="MVIRKKKCWDCGNAITHNTVCCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            5841..6410
                     /locus_tag="ACHKW0_002261"
     CDS             5841..6410
                     /locus_tag="ACHKW0_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7475092.1"
                     /translation="MTYKYNPFWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            6577..6978
                     /locus_tag="ACHKW0_002262"
     CDS             6577..6978
                     /locus_tag="ACHKW0_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7475093.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTKHGHECPYVRPERREVRLIKRLQQFVPDALPVVRKASWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            6966..7400
                     /locus_tag="ACHKW0_002263"
     CDS             6966..7400
                     /locus_tag="ACHKW0_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HFO7475094.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRRDVLCCVSCGCPLTLQR
                     GNDGQQPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVLDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVLSEDARADNPLQRL"
     gene            8163..8389
                     /locus_tag="ACHKW0_002264"
                     /pseudo
     CDS             8163..8389
                     /locus_tag="ACHKW0_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013364090.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
     gene            complement(8391..9173)
                     /locus_tag="ACHKW0_002265"
     CDS             complement(8391..9173)
                     /locus_tag="ACHKW0_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HFO7475095.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            9476..10396
                     /gene="rbsK"
                     /locus_tag="ACHKW0_002266"
     CDS             9476..10396
                     /gene="rbsK"
                     /locus_tag="ACHKW0_002266"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO7475096.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
     gene            10424..11740
                     /gene="fucP"
                     /locus_tag="ACHKW0_002267"
     CDS             10424..11740
                     /gene="fucP"
                     /locus_tag="ACHKW0_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015150 - fucose transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015535 - fucose:proton symporter activity
                     [Evidence IEA];
                     GO_process: GO:0015756 - fucose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HFO7475097.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            11752..12765
                     /locus_tag="ACHKW0_002268"
     CDS             11752..12765
                     /locus_tag="ACHKW0_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HFO7475098.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SVEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTDQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            13187..13387
                     /locus_tag="ACHKW0_002269"
                     /pseudo
     CDS             13187..13387
                     /locus_tag="ACHKW0_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(13687..14943)
                     /locus_tag="ACHKW0_002270"
     CDS             complement(13687..14943)
                     /locus_tag="ACHKW0_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HFO7475099.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(14956..15243)
                     /locus_tag="ACHKW0_002271"
     CDS             complement(14956..15243)
                     /locus_tag="ACHKW0_002271"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HFO7475100.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            complement(15259..15702)
                     /locus_tag="ACHKW0_002272"
     CDS             complement(15259..15702)
                     /locus_tag="ACHKW0_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011192680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HFO7475101.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            15973..17004
                     /locus_tag="ACHKW0_002273"
     CDS             15973..17004
                     /locus_tag="ACHKW0_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7475102.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            17401..17670
                     /locus_tag="ACHKW0_002274"
                     /pseudo
     CDS             17401..17670
                     /locus_tag="ACHKW0_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167450.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17844..19073)
                     /locus_tag="ACHKW0_002275"
     CDS             complement(17844..19073)
                     /locus_tag="ACHKW0_002275"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HFO7475103.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(19070..19801)
                     /locus_tag="ACHKW0_002276"
     CDS             complement(19070..19801)
                     /locus_tag="ACHKW0_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase FabG2"
                     /protein_id="HFO7475104.1"
                     /translation="MSRSVLVTGASKGIGRAIACKLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGARQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            complement(19801..20265)
                     /locus_tag="ACHKW0_002277"
     CDS             complement(19801..20265)
                     /locus_tag="ACHKW0_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HFO7475105.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCTTGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            complement(20262..21431)
                     /locus_tag="ACHKW0_002278"
     CDS             complement(20262..21431)
                     /locus_tag="ACHKW0_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HFO7475106.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPNTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAMISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFSRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            complement(21433..22017)
                     /locus_tag="ACHKW0_002279"
     CDS             complement(21433..22017)
                     /locus_tag="ACHKW0_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HFO7475107.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWEAQLPAGWTLRDNGDKRELRNTSGKL
                     VTEITYLNRQGKRVPIRIEQHVFKYHITIQYLGD"
     gene            complement(22014..24332)
                     /locus_tag="ACHKW0_002280"
     CDS             complement(22014..24332)
                     /locus_tag="ACHKW0_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HFO7475108.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGHYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKALKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIASYYPCGIAWVDRKSSF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            complement(24301..24906)
                     /locus_tag="ACHKW0_002281"
     CDS             complement(24301..24906)
                     /locus_tag="ACHKW0_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HFO7475109.1"
                     /translation="MKFLPLLALLISPFVSALTRDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDRTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            complement(24903..25325)
                     /locus_tag="ACHKW0_002282"
     CDS             complement(24903..25325)
                     /locus_tag="ACHKW0_002282"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HFO7475110.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVDEQSRELCFVSPDILFERMGVKP"
     gene            complement(25329..27005)
                     /locus_tag="ACHKW0_002283"
     CDS             complement(25329..27005)
                     /locus_tag="ACHKW0_002283"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7475111.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVSEQPGVTLIRLAENAGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKRATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPLDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMCHSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FTALLYPVVGVYWLTAARARKASQDWLARVRQQQPQAAKINSYQHFLRFGNAMLDKIA
                     SWRGELQLGRDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIEGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQHGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            complement(26996..27349)
                     /locus_tag="ACHKW0_002284"
     CDS             complement(26996..27349)
                     /locus_tag="ACHKW0_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HFO7475112.1"
                     /translation="MIRHEIERHLVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            complement(27336..28694)
                     /locus_tag="ACHKW0_002285"
     CDS             complement(27336..28694)
                     /locus_tag="ACHKW0_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HFO7475113.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNSMNKRVYAQLQELFHD
                     TP"
     gene            complement(28691..29272)
                     /locus_tag="ACHKW0_002286"
     CDS             complement(28691..29272)
                     /locus_tag="ACHKW0_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475114.1"
                     /translation="MSGIRSLPMIKLLTGLLLLVWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTYQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRRKMIKRKTP"
     gene            complement(29277..29528)
                     /locus_tag="ACHKW0_002287"
     CDS             complement(29277..29528)
                     /locus_tag="ACHKW0_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7475115.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            complement(29540..29797)
                     /locus_tag="ACHKW0_002288"
     CDS             complement(29540..29797)
                     /locus_tag="ACHKW0_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HFO7475116.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            complement(29772..30593)
                     /locus_tag="ACHKW0_002289"
     CDS             complement(29772..30593)
                     /locus_tag="ACHKW0_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HFO7475117.1"
                     /translation="MNKLMSRLGWAWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVRGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            complement(30590..31312)
                     /locus_tag="ACHKW0_002290"
     CDS             complement(30590..31312)
                     /locus_tag="ACHKW0_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HFO7475118.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSILFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            complement(31353..32411)
                     /locus_tag="ACHKW0_002291"
     CDS             complement(31353..32411)
                     /locus_tag="ACHKW0_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HFO7475119.1"
                     /translation="MYEQDTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDVARDSWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKK
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKLCIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            complement(32476..32865)
                     /locus_tag="ACHKW0_002292"
     CDS             complement(32476..32865)
                     /locus_tag="ACHKW0_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475120.1"
                     /translation="MKRYIKWFAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
BASE COUNT         8248 a         8010 c         8437 g         8192 t
ORIGIN      
        1 ctccccctga aacagtgcat tacagagtga ggatttaccg gccccgcttt tacccataat
       61 gccaatcacg ggttcgtgac tggtgagttt gcgcagatgt tccaggatgt gacgggaaag
      121 tgagtaaggc agggaggaga gcggtttttc aattgcctca atggcatcag acggattcat
      181 acatgtattc ccggtgaaaa aagacaaaac cccgcgcacc ggaaaggtgg cgggggattc
      241 acggagataa tatctgtctg aaattgtttt gaatcgatgg gattgttgcg tattaacatg
      301 aaggagatca ggacttatcc gttactgggt gatctccttc catgaaaata cgccgttatt
      361 gttaaacagc aggctgattt ctccgtaaag gggaaaatat ttatcggctg tatgattatc
      421 aggtgtgtct gtgtttactg ctgtgagtat tttttagtgt attgtggggg agatgtcagt
      481 gggagatatt aaatttacgt ttataattat cttacttctt ctgtgtgttt tttataagag
      541 tgcacacgta atgatatctg ttgataccat gtggttaagt gtttcccttc ctggtcatgc
      601 gtaatgatta aatgcttttg tgacaccatt cttccggtct gaatatttat cccgttcttt
      661 tttatttgtg cagtgatact gtcggaagag aattcagtaa tgtgaggtgt cctggtcagg
      721 ataatatctg ctgagatatt attagttgag tgtgttttta ctgagcaaaa gctggctttt
      781 aattcctgta ctgaaacact gtcgctcgtc cggggttaac tgaaactgga tgatgatgta
      841 aaatgaaggc tgtttttgtg tttgtcgaag aagtgttatt ttaatccatt ctcatcattg
      901 gctggcgatt atacagaagt atttgaagaa aaaatggtgc attttatctg tgtcagcagg
      961 gaagaagtat tttattgatg tatcagtatt aaatggtttt tgttatttat aaaaatgtca
     1021 tcctgtccat agaatattgt actcagcatt cagtgcagga actgtagtct tctttatttt
     1081 attcttcttc aggagggggg gtgattcctg aaggcgttat tcccggtgtt ctgtttatga
     1141 ttcttcccgg caggaaaccg gtatctgcaa tacccgggat gatgcttttt gttcgtacgt
     1201 ggttgtgtaa aatttgaata gtggatgtgc tccgaaggga aggaggggac acacagattc
     1261 attttgtctt tcttcagcag caaaatacac tgtggttatt atttaaccat taaatcaaca
     1321 aaaattttta cctcatattg atttgactgc tatttttgct attaatagcc agaattgcag
     1381 tgcttttata tctgtgtgta gtgaggttac tatgtcattc ggtcagcctt gtgatgagtt
     1441 tcccctgagt tcgctgcctc cactcattcg tgatgccgtt attgaagctc agcaaattac
     1501 gcaggctccc cttgggcttg tggcagcgtc tgccctgggc gctgtttcac ttgtctgcca
     1561 gaatctgatt gatgtctgca gactgaatac actgcggggg ccggtctctc tttttttcct
     1621 gacgcttgcc gagtccggag aacgaaaaac cgcagtggac aaattattaa tgaagcccct
     1681 gtaccagcag gagatgcagt tatattccag atacaaaagt gaactggctg tctggaaaaa
     1741 caaggaagag cttttaaagg ctcagaaaaa agcattgttg tcaaaactga ataaagaact
     1801 ccgcaaaggc gcggatgagt cagaaaccct cagacaactg gaagtacttc agaaaaacag
     1861 tgcggaagaa cctgtgaggt ataaattcat ttttaatgat gccacgactg cggcaattaa
     1921 gaatcagctt tgtggtaaat ggcgttcagt gggtattatg tcagatgaag ccggtattat
     1981 atttgatgga tataccctgt ctgagttgcc ttttattaac aaaatgtggg atggctctgt
     2041 attgtctgtg gacagaaaga atgaacccga acagatgatt gaaaatgcca gaatgacgtt
     2101 atccctcatg gtgcaacccg gtctttttga ccgatatatg gagcgaaaag gttctgttgc
     2161 cagggacagt ggttttcttg cccgttgtct tatcagtaaa cctgcaacca cgcaggggaa
     2221 acgctttatt aacggagctg tgattccggg aggatcatta acggcatttc atgaacgatt
     2281 aatggaactg gccagaggca gtattgaaaa atccagtgag gatgaacggt attgtctgca
     2341 tttttcgcct gaagcgcaaa aaatatttat tgagcactat aatgttctgg agcaggattt
     2401 atccccttcc ggacccttaa gcccgtttcg gggacatgtt tcaaaaaaaa cagaaaacat
     2461 tgcaagaatt gcagcgttat ttcagtattt cagctatggt gaagggaaaa tctctgccga
     2521 tatcatgacg tctgctgtcg taataagttc gtggtacaca gatgaatata aaaaattatt
     2581 tgcgttacct gatgaatctg aactgcagca gaaggacgcc gaggagctgt tcgactggct
     2641 cattgaggag tgccgcggag aatgcccgcc ccgtgtcagg aaaaattata ttctccagtg
     2701 tggcccgggc cggttcagaa acaggaaaaa actgaacgca ttgctgaaca ttcttgaatc
     2761 tcagttcagg ctttctgtcg tgccggaagg aaaaaccatg tatgttctgc ttccccagat
     2821 agccagttta aagttgtctg atgtctctgg tatttttacc tcgggttatc actacaataa
     2881 gctgagagcg aagtaaccgg tgagcagttt ttctccatta tgcttcagac ttagatgatt
     2941 gattattatg gggatttcat caccgatatt cagatgtatg aatatcggtg atgaactaaa
     3001 gctcaggagc tgcgtggtgg tgatgaatca acaggcatat aactgatacc cgccggtcag
     3061 aagttccagc ctgcgacttt cctgtctgcg tgcccgcagt ccggcgttca ggtgcagcgt
     3121 gtgacagaac gcacggagag cgaaacggca ggcagacagg ccgttcagtc agccctggca
     3181 ccctgcccgg ataccagctt ccctgaaaca actggcatca ggtgcgctta tcgtcaaccg
     3241 ttgtgcggta tgccaggagc acgtactgat ccatcagatc cgtcagtgct gcgctctgct
     3301 tctgacggtt gatgatccat tcctccagct ctttttctga tttccgtttc agattctccg
     3361 tccagtcgtc aaggcagttt ccggtcatta aatcaagcca gcaaagccag accagtttga
     3421 ggcgcaactc cgtgccacca aactgacgga agaggctgat cagtttattt gccagcgaac
     3481 gctgcctcag acgttcacat tcagacagga gaatgccttc cagcatcgga tgatgaacca
     3541 gcagatgttc actggtgcat aaattcacta tcagatcatc atccaggggc ggcgtggtcc
     3601 gtttgtccgg accataaatc agccggtgca gactctgggg tacgatggca atctccggtt
     3661 tttcaccatt gaatgacacc cacccgggcg tcagattatg gaagtagtag atattcggat
     3721 gacacacgcc ccaggatacg gcgtgccggg tgaagccggt cagccatttc tggtagcttt
     3781 cgtggagcag ctcgtaacgg gtctgatact gtggtttcat tttcatattc ctcacggggg
     3841 ataacaggca gcagaacgcc gccggcaggc cggagactgc gtctgctgca gatgaacggt
     3901 taagaattac ggacgggact gtgcaatacg cgcctgcagc caggcttcca cttcgctttt
     3961 cagccatcgg gagctgcggc ccattttgat gggcgcagga aagtcaccat ccttgatgag
     4021 tttgtaaaac cacttatcgg tcaggccggt cagttgagtg ataaatgcca tgtcgaccat
     4081 ctggtcatcc atcagtgaaa ctggggtgat catgagaatg ttcctccgga tgttgttaaa
     4141 ccagccggtg tggtcatgaa aacaacgcac caccttcacc ggcataacaa ccggggagaa
     4201 acgtaaaaaa taatcgggaa tgtgaccgcc aggagataaa cggggaggtt acggtggctg
     4261 agccgtaccg gtatgccggg tggttgttac gaaacgatgc ttccgcacgc gtgcgcgata
     4321 aagaagggat gattttcgtg aggaagaggg gaaaactggc gttctgataa ttgcagagtt
     4381 atttacgggg cggttacgat ggggcagacc gtgtccggtg ggcaggcgac aggtatcacc
     4441 gatgggataa gactgtctga ttggtattat aaatatataa gagagatcaa acacatgaga
     4501 tcaaatacat gagttcatat atgtgaaata aacacggtga gtgtgatcag tgggcatgat
     4561 gagaacgcag tagcaacaga ctgcgctatc cctgatagct ggcgtataac ccgcgtatat
     4621 aaaacgtatc tgcaatgtac gtgaacagag tgcattaagc atccgtctga gcggtaaaca
     4681 tatccgacct ggtgagtgac agtctgagag accggttaag tggtggctcg ggagtaatga
     4741 gtttggggtg ggtaccagaa tgccagtctg gcggtatgct gacagtatca cgttaattgc
     4801 agcataaagt gatgagaggc taatggaagt taaaaccaaa gcagttccgt cgttgttcat
     4861 cccgggagac gctggaaaaa gtgatttccc acacgcgtta taaacttacc tctgcggaac
     4921 tggaagcctt caactctgtg gttgatcact ggctggcaga actgacaatg aacaagcttt
     4981 acgatcgtgt tcccgcttca gtctggaaat atgtcatctg agcgttcggt aatcacaact
     5041 gcatcgcgcc aggtgttatc cgctttgtga ttcttattgg ctcttctcag gttattaaaa
     5101 gctgaatatg ttcctgttgt cgggaaccag gataataccg ggaacgttaa gcggtatagc
     5161 gacaacaaag aattcgaaat caaaagggaa tggtgccgga ggcgtgggcg gtaatgggct
     5221 gattttcagg tattaccggc ttacagcgtg tggttgatat tgaggggctg tgagtttcac
     5281 gtatttgtca gaaactcaga cgagttttcc tgtatgggac gaaataaggc agcggcaata
     5341 catggaaaaa tttaatatgg tatgaaggag aacctgtcgg ttacgcttaa tataaccggt
     5401 acactgtcag ctcaggggat acatgaaccg ttatctcacc agctacacag tatatacaca
     5461 ctgacgctga cagtagtcag caggaccaca ataatcagtg ccagcagtat ggtaacgata
     5521 cggtctgtat tccggtaata gccgaagggg tcgacagaac cacaatacgg gcaacacaca
     5581 gtattatgag taatcgcgtt tccacagtcc cagcacttct ttttgcgtat caccataact
     5641 ccctcctcac agccttaccc gtaaccggtt ttttacatta aaaaaacaac gctgaaaatt
     5701 caaccgtcag tccggagacg accgttcggg ttatctcaga gtgcctgaga tattgcctgg
     5761 ttggagatca cttcagggac tctgcgcatt ttttacggcg tggttaatcc gtatgaacga
     5821 aaccatacgg agatttaacc atgacctaca aatacaaccc cttctggcag caacgtattc
     5881 gtgagacggt gcggcacgca ctggatgttc atccccgtct gacggcattg cgggttgatc
     5941 tgcgtctccc ggatgtaccg gcagcaacgg acgcggctgt gatatcccgc ttcatcaatg
     6001 ccctgaaagc ccgaatcgac gcttaccaga aacgtaagca tcgggaaggt aaacgcgtgc
     6061 attcctcgac cctgcattac gcctgggccc gggagttcgg ggagcttaaa ggcaaaaaac
     6121 actatcacct gctgctgctg gtcaaccggg atacctggtg tcgtgccggc gattaccgcg
     6181 ctccggggtc actggccgga atgattaaac aggcgtggtg cagtgccctg ggagtggatg
     6241 tcgggtgcca tgccacgctg gtgcattttc cggcctggcc ggcggtgtgg ctggaacgcg
     6301 atgatgacac tggctttcag caggtgctgg aacgtgctgg ctatctggcg aaggagcata
     6361 ccaaagctcg cggcaccggt gagcgcaact ttggctgcag tcgtggctga gcccgacaga
     6421 cgacttatct gaccttagtg tgatgacgcg ttcacagcga ccacactgat tttcccttct
     6481 gagactgaac tgaaaaccgc tattcacacc tgaccacgct gcccgtaccg ggacggctgt
     6541 tgcctgcgtg tcgtctggcg gctgaggaat atgacgatgt acgcaaaatc ctttctcgca
     6601 cttgatggca acgggcgtct gactggtgcc cgtaccgcac agactgcacc ttacgatcgc
     6661 tacacttgcc acctttgcgg cagtgcactc agataccacc cgcaatacga cactgaacgt
     6721 ccctggtttg aacacactga cgacgggctg actaagcacg gtcatgagtg cccttatgtc
     6781 agaccggaac gcagggaggt acgcttgatt aagcgtctgc agcaattcgt accggatgcc
     6841 ttacccgtgg tgcgtaaagc cagctggcac tgcagacaat gtcaccacga ttattatggg
     6901 gagcagtact gcacaaactg ccagaccgga ggattcagta ttccccggac aactcaggag
     6961 gaaatatgcg aattctgaac tgttatatgg cgaatgacag caaggggcat tttgttacgg
     7021 cgaaagaagc agcgaagcac aaccgccggg acgttttgtg ctgtgtgtcc tgtggatgcc
     7081 cgttaacact tcagcggggc aatgacggac aacaaccgtg gtttgaacat gaccagatga
     7141 ctgtcgctga gaaaatcctg ctgcgatgca cctggcttga cccggcagag aaagaggccc
     7201 gtcgtttgca tctgcagggt atgacggttc tggattatac ggtgaaggtg agaaagtggt
     7261 tttgtgtgat gtgtgacgaa gattatgaag gagaaaagtg ctgcccgcgt tgtggtaccg
     7321 gggtatacag cagggcgtgg gggcggcagg aggtgctgtc ggaagatgcc agggctgata
     7381 atccgttaca gaggttgtaa tggttgcctc tggcgcagtt tgcggtgatg ccgttcccgg
     7441 gaatggattg ccagtgcggg gactgtggag cttaattccg gtgttggtgg cctttccgtt
     7501 gattttatgc caacagcccc cctgtgactg acaggctgcc tcgtcattcc attcgtgtct
     7561 ccagtaacag gattttattg tgccggttca ggcgtgcgtt tcccggcgcg tctgcaccgg
     7621 cttaaccaaa ttcaacaggg ataaaatagt gtaaataacc gaagtattac ttgcagaatc
     7681 gggggaagca tccggggttt tattcaacag gcatgggagg ctccggctct gacagacatt
     7741 gtactgtgga atgttgttac aggttggggc ggcaaccgtg ccaggagacc atgagccgtt
     7801 cacggcatta aaggccacac ttcagtcgtt tacattccct gtggtggcag gcatcttccg
     7861 ttaaggcgtc agagcgttac caacgttatg ttgtcatggc tcacctttca tagtcgatgc
     7921 ttatctggtg actgaaacta gccgcagata aagagtgtga agttgatacc atggagtgga
     7981 gtgacgtgca ggcaggagat tcgctctatg cagggttgtg agatgaattc aaaccggctg
     8041 tcagcgccag taatatttga agattcgtcc ggttgttatc cagtgtgcat taaaaaccca
     8101 gacattatgg acaatacaac ggcttccata acagggagga gcctgtttat tatccgggcg
     8161 taatgactaa cctttgtggg ctttttcccg tcgtgctttt aacgttgaag gcattttctg
     8221 cctgccgatt caggacagcg acaaaagcca tatcagacta ttggtcaatg atgaccagcg
     8281 tctggagtag atgataagcc aaattgataa actggaatat gtgatgaaag tgcaacgtaa
     8341 tcagtccaat ccgacgatgt ttaacaagag tacggtgttt tttcagtaag tcattcgtct
     8401 tctgaatcag gtgtaatgat ctttacagaa gcattccggc agtgcgtaac ataatctgcc
     8461 ggtaacggac gctcgctaat cagaacatca aatgcagaga gcggtgcgag gctggcagag
     8521 gctacttcat cgaacaaggc gtggcgggcg agcaaaattt tccttagtcc acgggccatc
     8581 gctttacgct tagtggcaag atcttcggga ttaaaccagc tgacgccaaa gtgatcatgt
     8641 acaccgctgg cggaaataaa tatttttcgc ggattgagag agtctaatgg cgaagagtta
     8701 ctggcatcgt aaaaagcatc acttctggca cgatacgtac caccacaaag tattgctgtc
     8761 acattaggtt tttcattcaa cgcaacaaag acacgatgtg agtaacagat gccagtgaag
     8821 gtgattgcat ccgggatcat gcttataacg agcggtatct cctggccatt atcaaagaag
     8881 atcagatcat tttcattaac cattccggcg gccagaattg caataggtaa gtcatcacga
     8941 tgatttctcg gaacgtcctg gattactggc atggatggtg cgggtttatg caccattaca
     9001 atatagccac ccagtagggt cagtggcaga ggttcatctt cctgatggag atcgcgacga
     9061 atagtcatta cagaaacttc cagcattcgt gccgcgtctt tcaaatggat tctgtcggtt
     9121 ttcttaagta tttcaatcaa acgtcggata cgctcttttt gcttcgtttc catcaactct
     9181 ctccgtaaaa tgttataaaa ataacattat ggcattagta aacattgttt tagttaccat
     9241 actatacgac cgaccctttg aaatgaaaag ggtatagact gctgaatttt cggtattaat
     9301 cctgttaggt tgcttatgat tagatagtgt tggcctgagt gataagactg gaagtctatt
     9361 aaataaacat cgggttgatg tgctaatcgc gatctcagtc acaaatgatg caaaagtaac
     9421 atgttaatgt tatttaaata ccattgttat gcgaaagttt ataagaggta gtgaaatgga
     9481 cattgcagtt attggttcca atatggtgga tctcattact tacactaatc agatgcctaa
     9541 agaaggagaa actctggaag cacctgcctt caaaatcgga tgtggtggga aaggtgctaa
     9601 tcaagcggtg gcggcggcta aattgaattc aaaagtcctg atgctgacta aagtaggtga
     9661 tgatattttt gcggataata ctattcgtaa tctcgaatca tggggaatta ataccactta
     9721 tgttgagaaa gtcccctgta ccagcagtgg tgttgcaccg atctttgtta atgccaactc
     9781 gagcaatagt atattaataa ttaagggagc gaataaattt ctttccccgg aagatatcga
     9841 tcgagctgca gaagatctga aaaaatgcaa attgatcgtt ttacaactgg aagttcaact
     9901 ggaaacagtt taccacgcga ttgagtttgg taagaaaaat ggtattgaag tgttattaaa
     9961 tccggctccg gcattgcgtg agttagatat gtcttatgcc tgcaaatgtg atttctttat
    10021 cccgaatgaa acagaactgg aaatattaac cggtatgtcg gttgatactt atgatcatat
    10081 tcgtttggcc gcacgttcgt tagttgataa agggctgaac aatattattg ttaccatgag
    10141 cgaaaaaggc gcattatgga tgacccgcga tcaggaagtc catgttcctg cattcaaagt
    10201 aaatgctgtt gatactagtg gcgctggtga cgcatttatt ggttgctttt cccattatta
    10261 tgttcaaagc ggtgatgttg aagccgcttt gaaaaaagcc gctctctttg ccgctttcag
    10321 cgtcacaggg aaaggtacgc aatcatctta tccgagtatt gaacaattta atgaattttt
    10381 aaccttaaac gaataatcat cctgcacagt taaaggtagc actatgaacg ataaaaacat
    10441 cattcagatg cctgatgggt atctgaataa gactcccttg ttccagttta ttttgttatc
    10501 gtgtttattc ccgctatggg gatgcgcagc tgcattaaat gacatattaa tcactcaatt
    10561 caaaagtgta ttttcgctaa gtaactttgc ttcagcattg gtgcagagcg cattttatgg
    10621 aggttatttt ttaattgcaa taccagcatc ccttgtgatt aaaaagacca gttataaggt
    10681 ggcaatttta attggcctga cgctgtatat tggtggttgt acactttttt tccctgcctc
    10741 gcatatggca acctacacta tgttccttgc agctattttt gcgattgcga ttggcctgag
    10801 tttccttgaa actgcagcga acacatacag ttcaatgatc ggtccaaaag cctatgccac
    10861 attgcggctc aatatcagcc agacctttta tccgattggt gctgcgtctg gtattttatt
    10921 gggcaaatat ctggttttct ccgaggggga aagccttgaa aagcagatgt caggaatgaa
    10981 cgccgagcaa attcacaact ttaaggtgct gatgttagag aacacccttg aaccgtataa
    11041 gtatatgatt atgattctgg ttgtggtcat ggtgttgttc cttttaaccc gcttcccaac
    11101 ctgtaaagtg gcacagacaa gtcaccataa acgcccgtca gcaatggata cgctgcgcta
    11161 tctggctaga aatccgcgct ttcgtcgcgg tattgtggcg cagttccttt atgtgggtat
    11221 gcaggtcgca gtatggtcat ttaccatccg tctggcgctg gagttgggcg atatcaacga
    11281 acgtgatgct tcaaacttca tggtctatag ctttgcctgc ttctttattg gcaagtttat
    11341 agccaatatc cttatgacgc gttttaatcc ggaaaaagta ttgattctct attccgttat
    11401 aggggcgctg ttcctggcat atgttgcact ggctccaagt ttcagcgcgg tctatgtcgc
    11461 cgtcctggta agtgttttgt ttggcccttg ctgggcgacg atctatgcag gaacactgga
    11521 tacagttgat aacgaacata cagaaatggc aggagcagtc attgttatgg ctattgtcgg
    11581 tgccgcggtg gttccggcaa ttcagggata tattgcagat atgttccact cactacagct
    11641 ttcattcctg gtttctatgc tctgctttgt ctatgtcgga gtctacttct ggcgtgaaag
    11701 caaagtacgt accgcactgg ctgaagtcac agcgtcctga ggagaatcgt tatgagtacg
    11761 cgtattaatt tgtggcgggc gctttttggc gagaaacctc gtatcttgct ggaaaatagt
    11821 gattttactg tcacgtcttt tcgatatgac agcggtgtcg aggggcttaa aattgcaaac
    11881 agtcgtggac atttgattat tcttccctgg atggggcaga tgatttggga tgcgcaattt
    11941 gatggacacg gcctcaccat gtgcaatatg tttcgccagc ctaaaccggc gacagaggtg
    12001 atagagacct atggctgttt tgcttttcat tcaggtttgc tggctaatgg ctgcccatct
    12061 gtggaagata ctcatctgct acacggtgaa atggcctgtg ccgcgatgga tgaagcatgg
    12121 ctggaattag acggagatat gttacgcctt aacgggcgtt acgagtatgt aatgggtttc
    12181 ggtcatcatt atctggctca gccaacagtg gtccttcaca aatcctctac actgttcgat
    12241 attaagatgg ccgtaaccaa tctggcttct gtagatatgc cactacaata tatgtgccac
    12301 atgaattatg catatatacc aaatgctaca ttcagccaga atatcccgga cgaaatatta
    12361 agattgcgtg aatcagtgcc ttcgcatgtt aatcccaccg ctcagtggct ggcgtttaat
    12421 caacggatta tgcagggaga agcctctctg tcgacgctga gtcaaccgga attttatgat
    12481 ccagaaattg tcttctttgc agacaaactg gacgcttata cagatcagcc agagtttcgc
    12541 atgatctctc ccgatggcac tacgtttgtt actcgtttct acagtgctga actaaattat
    12601 gtgacacgct ggatccttta taacggagag caacaggtag ccgcctttgc attacctgca
    12661 acctgtcggc ctgaggggta tcttgccgcg caacgtaatg gcacgctgat tcaggttgca
    12721 ccgcagcaaa cgcgcacttt tacagtcacc acaggaattg aataaccact caaggcctga
    12781 acagcggcgc gcttatcgtt aaggtaagcg cgttttttta cccgccagga caagactatg
    12841 atagcgttgc ccttatagac gatcacatta tcgtccactc caaatttgcg caactactgt
    12901 agagtggtgt actccacagc ttctttgagg agcttaacct ccattgtttt cttaccgagc
    12961 aagcactgca actcttttat ttatttgtag tggtcaacaa aaactggtca caatttcagt
    13021 ttttttccag tatctatttt ccgattcgtt cggcggtaat ccaccgttat attcatgagg
    13081 ccggagtgca ctataaaagt gtatgcgtaa accgactgct gtatgtagcc attaagccag
    13141 tattggtaac ataggtaccc accttttcaa ctagtggaca cctgttatgg aacaaaaaac
    13201 attatcagtt gaaccccgca ggtcattttc aaatgagttt aaacttcaaa tggttaaact
    13261 ggctttacaa ccaaggggtt ctgttgcccg tattgcccgg gagcatgata tcaatgataa
    13321 tcttctgttc aaatggctca ggctctcgct gatcaaaggg cgtatatcac ggcgtcttcc
    13381 ggtaacgaac tcttctggca ttggcgttga attattacct gtggagatga cgctggatga
    13441 gcggccttac cccgtttttt acgcgaggta attatgaaga tcaatgacta gccgtcgaac
    13501 cgtgtgcatg aactgctgca ctgaaacttc tcagctgtaa aataatcctt acgctacgta
    13561 cttctcggag cgcttacgat taatcggatg cagtcctgac tggaagaaag aaaaatggag
    13621 aaaataagag gaattaatat cctccacgtt cctgaaatcc tggaggatat taatgtcatc
    13681 aaaatattag cgaacgataa tccctaacaa aatacccagt gcgccaaagt cagcatcact
    13741 gaaggtggtg ttagcaaaac caatatcacc cagcaccggc aataagaata ctggcaggaa
    13801 ggtaataagc aagccctgag cgaaggcacc caaaatcgca ccacgacgtc cacccgttgc
    13861 attaccgaat acccctgcag ctgcacctac gaagaagtgc ggcaccacac caggaataat
    13921 cacgatcatg ttcagcagat acaacgtgaa cataccgatt aaacctgcag caaagctact
    13981 caggaaacca accagtacag cgttaggtgc gtaagggaag acgaccgggc aatcgagagc
    14041 aggtctggca ttcggaacca gcttgtctga aatacctttg aatgccggga caatctccgc
    14101 aatcaccatg cgcacaccct gcagaatgat gtacacgcca gctgcgaagg taatggattg
    14161 catgatagag aacatgaacc agtttttacc accgctaagc tctttcaccg catctgcacc
    14221 cgcaaacagg cacgtcacca ggaaaattat gctcatggta aaagagatag caaccggcgt
    14281 gtcgcgcaag aacagcagac ttttcggcac attcatgtct tcagtcgagt gctctttatt
    14341 accaaacttg ctgccgatga aaccggccaa tacataagat aatgttgaga agtggccgat
    14401 ggcaacgtca tcagaaccgg tcactttctt catgtacgga tgcgcaatag ccgggaaaaa
    14461 gaccattgcc acgccaacaa ccaaagagcc aacggctata agcgtaatgc cggtcatgcc
    14521 tgctgttgcc agaattaccg ctaccatcat cgacataaac aacgtgtgat gaccggtcag
    14581 gaagataaat ttccacggtg tgaaacgggc aatcataatg ttgataacca tcgcaaagaa
    14641 cataatcatc gccatttctt tcccgaagct tttctgcgct acggaaacaa tggcttcatt
    14701 gttcggcact acaccctgaa taccaaaggc atgctggaaa atgtttgcga agtcacccag
    14761 tgacgacaca actaaacccg cccctgcgcc taaaatcaca aaccccataa tggtcttgac
    14821 ggtaccttta atgcattcgg ttaccggttt tttctgtgca atcaggccaa tcagggcgat
    14881 taaaccgacc agcactgcgg gttcggaaag tacatcgctc atcaaaaaac gaaagaaatc
    14941 catatcgccc ccagcttaca gtgcgcctaa ttcggttaat accacggaca aacgttctct
    15001 catggctttc ttatcaatca tattttccag tgcgacgatt ttgcctgcga cagactgcgc
    15061 ggctaactgc tcggcaatgt ctttggtgcc gacatagata tcactcggcg tccctttcgc
    15121 ggagcccagg tcgacgtgat caacatcagc gctgacggac aaatccttca aaatgctttt
    15181 aatgctcatt tccatcatca ggctggtgcc taagccgtta ccgcaaacca ctgtaatttt
    15241 cataaatgtt cctctggatt aatagcgcgc aataacagac aaaatgtcct gcgtcgtttt
    15301 ggccgtcaaa atggcctgta tgtctttttc gttatcgaat aacttggcga gttgagaaat
    15361 cgcttcaatg tggctggtac tgtcggttgc agccagcaca atcagtaatt tcactggatc
    15421 gttttcatcg gcatcaaaat tcacacctga agggatcagg gtcagtgcta atgacagctt
    15481 gttagccccc tcttccgggc gcgcatgagg catcgcaata ccagggccaa ccacatagta
    15541 cggaccgata gtgtcgtgcg agtgatagat cgcatcaaca taacgcggtt cgatagagcc
    15601 gttttcaatc aacggtcggc atgaaatcgc cacggcttcc cgccagtctt tgacctgttc
    15661 cacgacctga acgacctctg tatttaatag attttttaac atgctactga taactcccga
    15721 tgataattaa caggagagta gatattttac gcattaatta atgtgatgat tgtcgcattt
    15781 gatagcgcta tctgataacg ctaccaatct ttgtgatagc gctatcattt ctctgccgcc
    15841 atgctttggc tatattcggt tcctgcatat acaatgcaac aggatatttg ctaccgtggg
    15901 caaaaactgg aaagagtaat ggtgaaataa aagtaatgaa aacagctggt tgtaattatc
    15961 catggagtcg aaatggaaaa aacacgtaag cgccgtggta ccggacgggt gacgctgcag
    16021 gaggtcgcta actttgcagg tgtcggaact atgaccgttt cccgcgcgct gagaacaccg
    16081 gaacaggttt ctgacaagct gagggaaaag atagaacagg ccgtagaaga gttgggttat
    16141 attcccaaca gaactgctgg cgcccttgca tctggtcaca gtcatacagt cgccgtactg
    16201 gttccttccc tgacagataa agccagctcc cgctttatgc agtcattaca gcaggtgctt
    16261 aataaaaatg aattccaact actgcttggg tgtcatgagt ataatcagct caaagaagct
    16321 gaaattctga tgacgcttct tcaaggcaac ccggcggcgt tagttatttt tggttctcag
    16381 ttggctgata aaactcacca gatactcgag aaaacaaata tacctaccat taacgttatc
    16441 ggttcccctt tcagtccggc tcaaattacc atcgaaacag cattttttga agcatcacat
    16501 aaactgaccg aacatctgct ggagcagggt tataagaata tcggatttat cggtgcgcat
    16561 atggacaacc gcctgcaacg tcagcaactg aacggctggc ataaagcaat gctgagccat
    16621 taccagaaca ctgatcttgt tatcaccatg ccggatgccg caagtctgca attggggcgt
    16681 tacgcattaa acgagatatt actgcgacaa ccagagctgg atgctgtgat ttgcagccac
    16741 gaagagattg cgctgggtat aatgtttgag tgccagcggc gtttgttaaa aatccctgga
    16801 aatatcgctg tagcttgtct tgacggttca gacaattgcg atcaaacaca cccaactttg
    16861 acatccatcc gtattgacta caaaaaaatg gggatagaga caggtaagtt actgattgga
    16921 ctattgaata ataatcatga tgaatcagaa gagtcgcgta tcgtgcaatt caactaccag
    16981 atagaattac gtcagagtac atgagcctaa aagagcgcta ttacaaagtt agctattgtg
    17041 gtagggatag ccgtttccgg ctattccccg cgcagttccg gatgtatgct gctaacgcat
    17101 ccattcctac ttcaggtatt tggcgtagaa gcggatgcta aaaacttgtc gcaaaaccgc
    17161 aatatcagcc tgcagtatcg ctctccagat tatccattcc tatattgatg acaacgacag
    17221 gtaagactgt gactcaccaa acttggatgt ttttctgagc ttaaagctcg ttgcgtgaat
    17281 aaggcgagag tcagcgaatc tcattgaggc ccgcagcacc ggctgcaata aggatataga
    17341 gcagcaatct accgtcaacg tcaaaaacac ggaagccttg caaacgggat gcgttcatat
    17401 aaacaacatg gagcagcaac agtacgatgc ctgttgttgc tcagtcagga ccttttctgc
    17461 catgcgcgag ccagcgtcac gattgataaa taatgttcgc aactgattac tttactgcaa
    17521 cagacgtggt tggtaataag cactggcgga caatgcagga tggaaggctg ttttcaggtt
    17581 gtccgttcag gtacgttaga tcagttcgtt gcgctaaaga caactgttca gtcccttatg
    17641 ttacccgtta tggcaaccat tgcccggtga taaccgggcg tgtcatctgt gcatccgttg
    17701 attcgtgtgg actgataaag ttttgtctgc tgaatcgtta tacggaatgc tcttcagtat
    17761 gcgtaagaga atttgaggtt catattcatt gattcagatt ttttgaagaa tcaccacatg
    17821 ggatgtccat gtggttgtat aactcagggc cagcgcttaa taatgatgga tgtattaatg
    17881 ccgccaaagg caaagttgtt actctgcaaa aactcacaat caaccttgcg ggcttcatgc
    17941 atgatgtaat ccagcgcgcc acaattcggg tctggtttat tcaggtttag cgtaggcgca
    18001 aaccaacctt cgcgcatcat ttgcagactc atccaggctt cgagcgcgcc acaggcacca
    18061 agcgtatgac caaaataact tttcagcgag gagagcggca cattatcacc gtaaatggtt
    18121 gctgtcgcca gactttctgc catatcaccg cgatcggtcg ccgtaccatg cgctgaaata
    18181 tagccgatat cttgtgcgct taaccctgcg attttcagcg attgctccat acaatactgc
    18241 atggtttcgc gctgcggctg ggtaatatgt gccgcgtcgc agttcgtcgc aaagccgacg
    18301 atttcaccgt aaatggtcgc gccgcgcgct ttggcatgct ccagttcttc gagaatgagc
    18361 gtacctgcgc cttcaccaat caccagtccg tcgcggtttt catcgaacgg tgaaggtgtg
    18421 gttttcggcg catcattgtg ctggctggtg gcaaaaaggg tatcaaacac tgctgcttct
    18481 gaagggcata actcttcggc accaccggca accatcaccg tttgataacc gtggcgaatg
    18541 gcttcccacg cataaccgat cgcctggctg ccggaggtac aggcgctgga ggttgggatc
    18601 acccgcccgc gcaggccgaa aaagaggcca gtattaacag cggtggtgtg cggcatcatc
    18661 tgcacatagg tggtgccagt gatgttgttc gtgtgctttt cagtcagcat ggtggcaaat
    18721 tcgctcaccg gaccggtact gccggtcgat gaaccgtacg caatgcccgt ttcgccgctg
    18781 gttaatatcg gatcgccaat caaccctgcc tgctctaacg ccagctcgct ggcacgggtc
    18841 gacatttgcg acacgcggcc catcgcacgg atacgcttgc gggtgtagtg ctccggtagc
    18901 gtgaaatcat caacaggcgc gccaagcagg gtgtgcagcc catcgtagac ctgccactcc
    18961 ggcattttgc gcaccgcatt ttcatacgcc agtaagcgtg cagaaacgtc ctgccagttt
    19021 tcaccaaagg cagtcacgcc gcccattcct gtaatcacta cgcgacgtgt cataacatcc
    19081 ctccgttgat ggaaataacc tggcgggtga cgtaacccgc aatatcagac attaaataac
    19141 tggcaagccc ggcaacttct tccgcctggc ccatacgttt catggggatc atcgacattg
    19201 cctctttcag agcgctttct tccatttcga tcatcccggt atcaataagc cctggcgcga
    19261 tgcaattgac ggtgattttg cgctttgcga gttcgatagc cagtgctttt gttgcgccga
    19321 taattccggc tttggctgcg ctgtaattca cctggccgcg attgcccatt acgccggaaa
    19381 ccgaggacag cgtgatgatg cgcccaccct ggcgcgcgcc gatcattggc ataatgcacg
    19441 gctgaatgac gttgtagaaa ctgtcgaggt tggtgtggat caccgcatcc cagtcgtcat
    19501 cgcttaaagc cggaaaagcg gcatcgcggg cgatcccggc gttactgacc acgccgtacc
    19561 aggcaccgtg ttgcgcgatc tcatgctcca gcacttcccg acactgttcg cggtttgcga
    19621 cgtcgaaact gagcaaacgc ccgttacccc cgttggcgac aatggcattc agcgtttcct
    19681 gagcgcctgt ggcgtcacgg tgatagtgaa cgccaatgtt aaagccgtct gccgcaagct
    19741 tgcaggcgat ggcgcgacca atgcctttgc tggcaccagt aaccagaact gaacgactca
    19801 tcaggacgct ccttgttgaa acagggtggt aagttcttcc gcggtcggct ggaaggtgtt
    19861 aatgcggccc gtcgcctcgc cgtcgttgat gctgcactca aagctgccga agcgatcgtc
    19921 ctgcatcagc agtttgacgg taatggtcag cgtctggcct gcgggcagaa tgccagtggt
    19981 gcaaagcaat tcacgcgcgc ccagtatcat ccctaactca atactgtttt tgccctgctg
    20041 gtggcgatgc cagccagacc agacgccgac ggtttgcgcc atcagttcaa gggcaaacca
    20101 gccagggaga ttgccgtccg ggtcgagaaa cggtgccagt acgccactgg gcgaaaccgt
    20161 cacgcggcac accgcgctgt catcgctgac gctcaccacg tcttccagca gtaacatggg
    20221 cgcgtcgtgg ggcagataag cgccggggga taaatagtgg ctcacgaaac cctcccgagc
    20281 agaatgctgg cgttattgcc gccaaaggcg aaggaattgg acaggatcac cggacgtgcc
    20341 agcggctgcg gtttttcgat aatgccacac ggtggcagcg tgggatcgcg cggcgacagg
    20401 ctgaagtcct gagcgggcag cggtaaatca cgctgtaaaa tcaacatact aattgccgcc
    20461 tcggtaatgc cagccgcgcc gagcgtgtgt ccggtcaaat gtttggtgga gctgcacggt
    20521 acgcgttcgc caaacagggc gttaaccacc attgattcaa tctggtcgtt gagctgtgta
    20581 gcggtgccgt gcaggttgat atagccgacg tcatccggcg taagctgcgc atcggtcagc
    20641 gcctgattaa tagcgcggat cgctccttcg ccctgcggat gcggggctga aatatggtaa
    20701 gcgtcgctcg attcaccaac gcccagcaac gcgatgggct gcggttcccg ggtcagcagc
    20761 attagccccg ccccttcacc aatggtgata cctgcgcggt cacgactaaa cggctgacat
    20821 aaggttggcg agagcgattc gaggctgtga aaaccgttaa tcggcattcg gctcagggtg
    20881 tctgcgccac ccacaatggc ggcatcgacc agccccgctt cgataagcct gcgcccgctg
    20941 atcattgctc tggcactgga ggagcaggcg gttgaaaggg tataagccgg gccgtcgagc
    21001 gccagccagt ggctgagaaa acgcgacgga tcgccgagtt cttgttgtgc atagtgccac
    21061 gtggtgttcg gctgcccgtg ggtgtgcagg ttaacgtgcg tgtcgccttc atgcaggccg
    21121 gaggtgctgg tgccaagcac aatcgcgatg cgctcgcagc catatttcgc gatagcgtca
    21181 tcaacctgcg gctggagctg cgccagcgcc gccagcagga tctgattgtt gcgcgagcga
    21241 tgcgcagcga atttttccgg gataagcgga agctcgccat ccacgccagc caggaccgcc
    21301 tgcggatgcc cctgcaacca gcctgcgcgt gggcgcatgc cgggggcgac accgcgagcc
    21361 aagttggctg cgatttcgtc aaggttattt cccagggcgt tgatcatgcc gacggcggaa
    21421 atataaatca tatcagtcac ctaagtattg aatggtgatg tggtatttaa agacatgctg
    21481 ctcaattctg attggcacgc gttttccctg gcgattcaga taggtgattt ccgtgaccag
    21541 tttgccgctg gtgttacgca gctcgcgttt gtcaccgttg tcgcgaagtg tccatcctgc
    21601 gggaagttgc gcctcccagg cgctaatcgg ccagtggctg agcatcacgt cagccagtac
    21661 ctgacttgcg ggcggtaact gtgggacgac gatggattgc tcggcgcgta gcccttgcgc
    21721 atcgtaggtc accagaaaca ggcgaatgcc gaccgacgac agcccggcaa gggtgatttt
    21781 ctgatcgtcg gcgttaagca tcactaacag cgactgggtt ttgccgttga agctgccggt
    21841 caacagttgc tgcgaattga ctgcgggtga aatccctggt gccggcagcg tgatgcgcgt
    21901 gccaggttgc agccacgcct gcgggcggcc ttcctgttct ggttgcgagt ggctacagcc
    21961 ggtgaggata agcgtagcgg tcagggcgaa cgctcgccag aatgtggatt tgatcatttt
    22021 tttgttctct ttttatcggg catagccagc ggcgaaagca ggaaggcggt gaaaataccg
    22081 ctcaccagca caatgccaaa actgctgatg gcctgggtgg cgctgaatac cagcattccc
    22141 agcgtcagca aggtggtgag cattgccagc gcgatcgcca gtagcgaagt taacggtgta
    22201 ccgcgcggat tactgaaaaa cagcgtgtag ttgataccga tgccaagcac cagcaccagc
    22261 gccagcagcg aaaagagatt caccgcctgc ccgctcatcg ccagcaccgc cagaccacag
    22321 cccagcgaaa gcaccgaagg caccaggctg ataagccctt tgcgccagcc gagacgggcc
    22381 actgcgccgc aggcaatcac tgccagcgcc accagcaaca agccggttaa gacgtagcgg
    22441 taaagtgcga acaattcatc aaaggagctt ttgcgatcaa cccaggcaat gccgcaaggg
    22501 taatagctgg cgatttcctg caacaatgcg ctacttttaa ccccttcaac cggcaccagt
    22561 acaccgcttt cgccgttttc cagcgtcagc cacagcagac gccagccttc actggcgggg
    22621 cttgccagcc attcatcgac cttcaccggc atggcgttga gatctggttt caccgccgtc
    22681 agccctgcat tttgcagtgc ttttgttacc gtcggggcag ccgttttcag caggtctaag
    22741 tcttcctcct gccgcgccag ggagttcagc ggaatagtgc ggtagttgct gataagcccc
    22801 tcttttttcg catactcaag tgaggcggta tatttctcca gtcgccgcaa tgtttgctgt
    22861 ggggagtcgc cgtaaactac aaaccatttt tgatcgacgc tttgtccggt cagggcggta
    22921 atggctttct cctgcgccag aatgtgctgc ggcagcgcct gtaactgcga gatatcgtca
    22981 tcgacgcgca gcattgacat ccccgccagc gaaaacagcg ccagcgcaac gggcagaccc
    23041 cgcgacagtt ttttattgcg ccgccacgct gccagccagc gtagcatcag cgccatcgcc
    23101 ggaaccgggc gcaccggcag accacggcac agccacggat gccagaacag gacggtcaga
    23161 caagaggcgc tcaacccgac ggcggcaaaa atcgccatct ggcgaatgcc ggggaagggg
    23221 gcgagcatca taatcagata tgccgccacg gttgtgagca gcgccagcag cagggcattg
    23281 cgtactttcg ccaggctttg ccacggcgaa acgtcgttgc cgtgcaccat ccgctcggtg
    23341 agataataga gcgtgtagtc agcggaaatg ccgataacgc tcatgctcat caccagcgtc
    23401 atcaggtgta attcaccgaa aatcaacaat gtggcggccg ttcccgccag cgcgccgatg
    23461 ccgatggaaa tcacgcaaag cagcaacggg cgcaaagagc ggaacaccgc cacaatcagc
    23521 aatatcaccc ccagcagcgt tgccacgccc agggtggaga tatcctgctt cgcctgttgg
    23581 ctggcgtaat cgctgtaaaa caccgtgccg cgtgagagca actgcgcctg cgggtaacgc
    23641 gttttcagag ccttttccag cgtattcagg gtcgtgatga gctggtgggt ttgctgcata
    23701 tcaaacgacg atcccgccag ttcgccgtgc agcagatacc agtaatggcc ctggggatcc
    23761 tgcgtcacca gccagccgtc cattagtcgt aaacgctggc catttttcgc catcgccagc
    23821 tgcgagccgc gcattaacat cagcggatcg ttttgcagct ccttgccgct taccccggag
    23881 aacgcagaat aaagctggga gaggatccac ttcgcctgcg cttcgccgcc attttgcagg
    23941 cgggcgcggg tgtcggggtc aatcaggccg ttgcggtgct gccagaaaaa cgctccccac
    24001 gcctgctggc tggcggcatc cattggccct ttaatgtcgc cgagcgcagc ggatttttgc
    24061 agcagcgtca gccactcctg agccaccctg ggattagcct ctttaccggg gctgaccagc
    24121 cacaccagtt ggcggtccag acgctgcata aagccgtcat tcagcgccgg aggaatatcg
    24181 cccatcgcct gtttgggtaa catagccagc acgctactgt tcagtcgtga ttgtggcagc
    24241 aggatcagca acgccgccgc catgaccagg cagactagcc cccataacag cgcggggcgt
    24301 ttactgggtg gcaaagcgtt ggcgttcgtc atcggtcagt tgcgctggtg tcagttgatg
    24361 ttgggtaaga gcgatatcgg tgcgatcgcc ctgtttatca ttaagctgaa tgctctccag
    24421 ataggtttgc ccggcgagat cgatggtgtt gaaaattttg tccagcggcg tggtggtcgg
    24481 cgtcaggcgc agcgtccagc ggccttcgcc tttgtcggca aattcgacgc ggaagttttg
    24541 ttccagcact ttgcgatcgg cctggaacag cgcgcgcagc aggtggttaa actggaacat
    24601 ctgtgggttg ttttctgccg tgatgatttg cggcggctga ccgttgatca cctgcaccat
    24661 gcgtttatcg tccagtaata gttgcatggg gaacggtgag gtttgatccc acaataaccc
    24721 ctggtcgcgg gcgatcaaca tctgaccctg agatcgcagc ggctgcggca gatctttaat
    24781 cgtccgggtt tgatcgaaat gggcgcggat caccggttgt tcggtaaagc gttgctgaag
    24841 atcgtcccgg gtcagggcgc tcacaaacgg gctaatcagc agcgccagca gcggtaaaaa
    24901 tttcatggtt tgactcccat gcgttcaaac agaatatcgg ggctgacaaa gcacagttcg
    24961 cggctttgct cgtcgactgc gacctgaatg gtgtatccgg tggtggcgcg tttgcccgtt
    25021 tcggcatcga aaatctgata gccaatccgc agacggtttt cgaactcttc gatatgcgca
    25081 cgcacgcgga tgcgttgctc aaaggtgagg gcgtggcgat atttcacccg cgcatcgacc
    25141 actggccaca aataaccgga ctctttcatc tgccgatagc cgtaattgaa ctggttgagc
    25201 agcgcctcgc gggccacttc aaagtagcga aaatagttac cgtgccaggc cacgcccatc
    25261 atatcgacgt cgtgaaaggg aatggtcagc tcgacttcag cggtaaatcg gggatcgtta
    25321 agcacccttt actccttgtc ctgaatttcc ggcagttgcc agaaatcgaa aaaattaaac
    25381 cagtcgaggg gcgactggag cgcgtaatgt tccagacgcg cggcgtagtg gtcgatagcg
    25441 ttttgcagcg cctgttggcg ttcgccacgc ggcaacagca gcgggtcggc aaaggtttcg
    25501 cagtgaatat gcagcttgcc gtgctggcgt agggcgaaaa tcagattcac cgggcagcgc
    25561 aaaatagagg cgagaataaa cggcccctgt gggaagggcg caggctggcc cataaacgga
    25621 ctccagcaga cgcgccagtc gccgccgcgt tgcggggtga cggcgatgcg gtcaccgaca
    25681 atcgccaccc attcgccgtt atccagcttc tctttcagca ggatggcggt ttccgggccg
    25741 atatctgtta ccggcatcag gttaatgcct gcctggggag ccatctcctg cattatctgt
    25801 ttaaagcgtt gggcgttttc gctaaacacc agcgcgttaa tggttttgta gccctcaatt
    25861 tttgccagcg cccggcacac ttccacatcg ccaagatgtg aggccagcag caatttgccg
    25921 cgcggatcgc ggacgtcaag cgccgcttct gcgcctggcg caaacagcac atcacgcccg
    25981 agttgcagct cgccgcgcca gctggcgatt ttgtcgagca tggcgttgcc gaaacgtaga
    26041 aagtgctgat agctgttgat ttttgctgcc tgtggttgtt gttggcgcac acgggcgagc
    26101 cagtcttgcg acgctttacg cgcacgagca gcggtgagcc agtagacgcc aaccaccggg
    26161 taaagcagcg cggtaaacgc cgttcttccc agcagacgcc agaccagcag catcaggcgc
    26221 attccccata accctttaac ttcactctgc cgcgcccagt ggctggaaga gtggcacatc
    26281 agcagtgaag gaatacgcgg caacatgccg aaaaacagac gcgtgtgcat gagcgagatg
    26341 cggacgttat ctttcagggc atcaaaatgc gaaagcccgt ccagtggata ggtgacgcgg
    26401 gtcggcacga aatagctggt atttccctgc cagtagaggc gcaccatcac ttcggtgtcg
    26461 aaatccatcc gcttgccgat ggtggcgcgt tttgccagtt gcagcgtcgg cgcaaccgga
    26521 taaacgcgaa aaccgcacat gctgtctttc agttgcaggg agagcgtttc gatccatacc
    26581 cagacgtggg tgacccagcg cccgtacagc cgtgagcggg gaatggagtc atcgtaaatt
    26641 ggctgaccgg agatcagcgc cacagggtgt tgttcagcga gtgccagcag tttggggata
    26701 tcttcaatcg catgttgacc gtcagcatcc acctgcaccg catggctgaa ccctgcgtct
    26761 gccgctgcct gcaagccacg catcaccgct gcgcctttac ctgcgttttc tgccaggcga
    26821 attaaggtca cgccaggctg ttcgctaacc agattatcca gttgctgttg cgtggcggca
    26881 tcgctgccgt catccaccac aatacagggc agattaaatg gcttaagacg cgccagcacg
    26941 cccggcatca tcgcgccgtg gttgtagcag gggatcaaca cgcagggaga aaagtttacc
    27001 gacatagacg gatcttcccg ctactggcgg tgtggcgggc gtcaccgtcg tgacgctgat
    27061 agcagaaagt gagtacctga cttgtttcct gccatgttaa ttgcagggta acagtcgtct
    27121 caggtataag tggcgccaga aatttaacat tctggatact acgaaaacgc cagcccggag
    27181 caagcagcgt ggtagcataa tgcattaccc agtccaactg ggccacgcct ggtaacagag
    27241 gctgtacggc aaaatgacca ttaaaccagt agagcatcgg gtcaaggtat agaacgattt
    27301 ccatctcctg cggttgaacc agatggcgct caatttcatg gcgtatcatg aaataactcc
    27361 tgtaattgcg catagacacg cttgttcata ctgtttactg gtatttcatc aataatgcgc
    27421 cagtaacgag gtactgcaac aggttcaagc catggtaata gtgctttgcg ccaggacaac
    27481 tccagagttt tccctccgga gcattgccat atttgacggg cctctttatt tggtacaacc
    27541 acgacaccga tagcctggcg gccattgcgt attaatggta ttgcagcgac atccataata
    27601 ccgtcaagtg ccagaagtcg ttgttcgatt tcaaccagtg atatccgctt ctcctctatt
    27661 tttaccactc gaccacgtcg tccgatcagg tggaactgac cgttttcatt gaattgcaac
    27721 atatcgtcaa gcaccaatcc ttcagcaggg attagtggcg aaaagacacg aaccccctca
    27781 ctttcctgtg aaagatgaac atcagaaaaa gtaaaccagg ggatattatc ctgttgacgg
    27841 taacgccatg cgagaatacc tgtttcggta ctaccataga tttcatcggg ccatatattc
    27901 agccaggtgg aagttttctc tacatcctgc catggtagca taccgccggc agagagaatc
    27961 atttccactg ggggaggcgt cagatgaagg tcgagtcgct taaggaacgc cgggctgctg
    28021 acaaaggcat agcggtaggt atggcttaat gcggcaagtt gctcagaata ccagagcatt
    28081 gcggcatgca atggcaggcc aagtgccatg ggtaaaaaaa tacggaaagt tagcccatac
    28141 aggtgctgag gcatcacgga tgctacaaaa cgacagccca gtagtcggtc tgcaaagtga
    28201 gttgccagca tgtttgcttc ttgatcaagg ctgataacag gttttgcaat ttgttttggt
    28261 tgtccggttg aacccgatgt gaaaagttca ataaacgcgt cagaagcaat atcatcaaag
    28321 gctatcgtgc tggtcgtcat ctggtgagat gtgcttacaa caaactgtgg accgttccag
    28381 cccagccttt tatcactcag gacgcaatca aacaaggagc gttgtacatt taacaaagcg
    28441 gcgcgattat ggcccgggat taccgggatt ttacctgagt gtagcgtggc caacaacgct
    28501 acaataaaaa gatagctgtt ttcaaaacaa agtgcccagc gattgcctgg ctggtcgcgt
    28561 aaatggtcga tcaaaagcgc tacatcatgc cgcagatgcc ccagtgtcca ggtatattcc
    28621 ccaagccagg caacaggagt tgttacaggg cgagaggctg tcaaccactg tgaaagtgag
    28681 agagtctgat tcatggtgtt ttccgtttga tcatcttacg acgtatcagc cattcccctg
    28741 cagtcaggaa acctatcaga aggtaagcaa tgataccgtt ccaggctgtc cacaagggca
    28801 tgttgccata caaggtggta aataacgcaa tactgccatt gaaaataaaa aacaggcacc
    28861 agacctgcgt tacgcggcgg gtatatcgca cggccttttc cgggaggtct ggctcctgca
    28921 aacgtgccag ccgctcaact atcggcattg tcgaccgcag cgaaccgcca aaaaccgcca
    28981 gcatgactgc gttaaccact accgggtaaa acagcagtaa ctggtacgtt ttcaacagaa
    29041 aactggagac acacaataca atgcccacaa cagcaacgat ttttgtgacg acctggagcg
    29101 gccccgtctg gcgacggggc tggcgcaggc gcaataaaag caacagcgcc atcagcggca
    29161 acagccagtg caggctgtta tgcaccaaac ctagccagat taaaaaaggc cagacgagca
    29221 gcaataatcc ggtcagcaac ttaatcatcg gtagcgagcg aatgcccgac atcaaattag
    29281 ccttcttgta gcaggcgttc tacagcctcg acaacatcct ggactgtacg cactgcttta
    29341 aactcttccg gcttgatttt cttaccggtt ttcttttgca ggtgcacaat catgtcaacg
    29401 gcgtcgatgc tgtccagttc cagatcttcg tacagtcgcg cttcaggttt gatgtcctgc
    29461 ggatcgattt caaacagctt aaccagcagg gctgagactt cctgataaat ggtttgttgg
    29521 tctgtcatga tggttttcct caggcacgtt gcgcagcaat gaaagaggcc agagtggcga
    29581 cggagaaaaa gtgctgacgc atttcttcgc tttccgcaga gagcacgatg ccgtactcat
    29641 tttttactgc cagtcccagt tccagtgcat cgatagagtc cagccctaaa ccgtcgccaa
    29701 acagtggcgc attgatatca atatcatcag gagtcaagtc gtccagattg agggtgctga
    29761 tgatgaggtt tttaatttcc agataaagcg cttgcattat ttattcctga tagaggtaaa
    29821 caaccttgct gtaattgaag caaaagatgc cggtttaact gtcttgcagc cagcgtcggc
    29881 tcttgtgaag ttgcatcgta aaatttattg atttctaccc gcccacggac ttcaaccgta
    29941 aaaaacggcc tggcaggcgg gacgtcatac cacttacttt gtttatccag cagatgctcg
    30001 ctacagccga tcgtcacgat gcgtaaatcg ctggtgcagc gcacggcaat attcgctgca
    30061 ccgcgctgta acgtcatttt ttcaccgggc ttcgttcgtg tgccttcggg gaaaatcagg
    30121 atagtgtcac cctgtgccag tcgctgttgg cagcggggta acagggtttc tgcttcgctg
    30181 ttgattaaat aatctgccgc tcgcacgacg ccgccaagaa aaggattttt taacagcgcg
    30241 cttttcacca ggcagtcagt ttctggcatg actgatgcca gcagcacgta atcgatcagc
    30301 gtgggatgat tggctacgac gaggcaaccg cgttcctgac gtaaaatttc agcgccatca
    30361 atacgataat ccagcacgcc aagccctttc gcgacagtta aaaacaggcg aaaactggcg
    30421 gcaatgctgc gacgtgccaa acgacggcgg cgggaagtat cccacaccaa aactaacaat
    30481 atgttaaacc acacgactga aagcagcagg ccgccgagac caaacaggac aaaacataac
    30541 ccggtcatga ccagtcgcca tgcccatccc aggcgcgaca tcagcttatt catctgcggc
    30601 tccagcgcca ttgcacgcgc tcgccaggca gtgaaaaagc atctgcattt tgtaaatagt
    30661 gctgtaaaaa caagatgctt tgcggcagtg ttgtttcatt taccgcaatg gctggctgcg
    30721 tttcacattg ccagtcatcg ccagcttcaa tcaccagcgc gacggcgtac ggccaggtag
    30781 gcatttctgc gggcagttgc gggtgataaa actcaggtag aaagccatca aaatcgacca
    30841 tcaacacgcg ctggtatccc gcttgtagca ggctaagcac ttcacacaaa ccttgctgga
    30901 aactgtcgcg accagccgaa agcgatgacg aaacgatcgg ctgtttggcc acaatggtca
    30961 ggttacccac ggaagagttg tgtacggaaa gcgcgaagtc cgtcggagaa agcgcctgtt
    31021 cggttgccag tgcatgaaca atgcggtaat tgcgctccag ttcgccatga cgactggtat
    31081 acaaaacagc gtctggctga tggcggcgta gcattgtcag accgcattca acagccagtt
    31141 tgctgcctga actgagacga cgagcggtca tcattggcag ttcacttaat tttgccaacg
    31201 gcgcggcagg gtcaatcgcc cacggctggc gcgaccatgc ttgccattgc tgcacatcgc
    31261 taagtccagg cgccagtgcc tgccagttgg taatatttaa tgcaaatttc atcaacgcgc
    31321 gcccgcggta aaccaaaatt attaagtgac gattatttct tttggcatat caaaagtgta
    31381 tgcccaacgc ctaaattatc gtgacgctct gctacctgga atcctgcttt attcagatag
    31441 tcgtaaaatt tctctgcact atagaaacgg ctgttaccgt ttgccatgca ggtaaaataa
    31501 agtgaggatg cattcaggct aaacgatgtc gcttcaaacc gctgtgcatc ccagaacaac
    31561 tccatgatgc aaagttttgc gccaggcttc attacgctgg caattttgct gagtatgcta
    31621 attatttgtt cgggtgaaaa acagtctaaa aattggctca tccaccagat atcagcctcg
    31681 cctggtaatg gggcatcgct tagcatatcc accgcatgga aatctatgcg atcagaaaaa
    31741 cctgcattcg cgatattttt tttcgccagt acgatttgtt gtggcagatc taataacgta
    31801 acggcgatgt tttcattgaa tttgcagcag cgcaaagccc acttacccgt attgccaccc
    31861 acatcgtaca aggttgtggg gttgttggcg aatacataag gcagtgcggc gttaaatgcg
    31921 ccatctgaat agtaatgatc aaacgcaaac cagctatcac gtgcgacgtc tggtaattgc
    31981 gaaagtgccg gatatatcgt cggccagtcg ccaaatactt ttaacccgga aggttttcct
    32041 tcattgagtg aatcggcaag gaagaagagc ccctggtaac agacatcctg ggtgaaatcc
    32101 atattcacgc gagtcatagt gtcatgcagg aggtaatgac cgattttcgc caggtaataa
    32161 attccctctt tacaggtaat aattcgcccg cttaagccca tatccagtaa cacacccacg
    32221 gcatattcat tcaggactgt gtgctcagta atcgcattaa ggggagctcc ctgtttaccc
    32281 tgttgatcca aataatctaa tattcctgca ttgcgcaggc acaaagctgc ctggaaaagc
    32341 ataggagcaa aggcaatccg ctgtgcttcg gtgatggcat ccagtgcgct gagcgtgtcc
    32401 tgttcgtaca tatataacca ttcttataga gaaagttaaa tactatcggg ccattccagc
    32461 ccgatatcag catcactata acgttgctcc tgtagataag ttaacctgta tatctttatc
    32521 taggttacgc acccagcggt tatagttttt atgaattttt ccatcagaag cctgcagatt
    32581 caggctactg tcatatttga tgttatagta ggtagctgta tatgtaatac gaacctctgc
    32641 aacgtgattt cgtgtttgat agcgagcttt aataactcca ggggacactt gcgtcataat
    32701 ccacttgcgc tgcgcacctg ctttcaaaat ggcattttta acctgctcct gagtatgacc
    32761 cacactgaca gtggtgctta tctgttgcac tggggccgta cgggcacagg cactcaacat
    32821 actgataaaa attgtgatgg caaaccattt tatataacgt ttcattagtg cctcttatgt
    32881 atgcaat
//
LOCUS       DAZFXT010000046        32546 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.046, whole genome shotgun sequence.
ACCESSION   DAZFXT010000046 DAZFXT010000000
VERSION     DAZFXT010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32546
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.046"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            53..655
                     /gene="yebB"
                     /locus_tag="ACHKW0_002293"
                     /pseudo
     CDS             53..655
                     /gene="yebB"
                     /locus_tag="ACHKW0_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
     gene            complement(657..1178)
                     /gene="ruvC"
                     /locus_tag="ACHKW0_002294"
     CDS             complement(657..1178)
                     /gene="ruvC"
                     /locus_tag="ACHKW0_002294"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HFO7475121.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1213..1953)
                     /gene="yebC"
                     /locus_tag="ACHKW0_002295"
     CDS             complement(1213..1953)
                     /gene="yebC"
                     /locus_tag="ACHKW0_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7475122.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1982..2434)
                     /gene="nudB"
                     /locus_tag="ACHKW0_002296"
     CDS             complement(1982..2434)
                     /gene="nudB"
                     /locus_tag="ACHKW0_002296"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008828 - dATP diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0019177 - dihydroneopterin triphosphate
                     pyrophosphohydrolase activity [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HFO7475123.1"
                     /translation="MEDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2552..4324)
                     /gene="aspS"
                     /locus_tag="ACHKW0_002297"
     CDS             complement(2552..4324)
                     /gene="aspS"
                     /locus_tag="ACHKW0_002297"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HFO7475124.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4634..5200
                     /gene="yecD"
                     /locus_tag="ACHKW0_002298"
     CDS             4634..5200
                     /gene="yecD"
                     /locus_tag="ACHKW0_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7475125.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5197..6015
                     /gene="yecE"
                     /locus_tag="ACHKW0_002299"
     CDS             5197..6015
                     /gene="yecE"
                     /locus_tag="ACHKW0_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HFO7475126.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DGRPVHAACPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            6068..6463
                     /gene="yecN"
                     /locus_tag="ACHKW0_002300"
     CDS             6068..6463
                     /gene="yecN"
                     /locus_tag="ACHKW0_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HFO7475127.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6504..7247
                     /gene="cmoA"
                     /locus_tag="ACHKW0_002301"
     CDS             6504..7247
                     /gene="cmoA"
                     /locus_tag="ACHKW0_002301"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HFO7475128.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7244..8215
                     /gene="cmoB"
                     /locus_tag="ACHKW0_002302"
     CDS             7244..8215
                     /gene="cmoB"
                     /locus_tag="ACHKW0_002302"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HFO7475129.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRVVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8380..10809)
                     /gene="torZ"
                     /locus_tag="ACHKW0_002303"
     CDS             complement(8380..10809)
                     /gene="torZ"
                     /locus_tag="ACHKW0_002303"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HFO7475130.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(10834..11934)
                     /gene="torY"
                     /locus_tag="ACHKW0_002304"
     CDS             complement(10834..11934)
                     /gene="torY"
                     /locus_tag="ACHKW0_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HFO7475131.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            complement(12322..13068)
                     /gene="cutC"
                     /locus_tag="ACHKW0_002305"
     CDS             complement(12322..13068)
                     /gene="cutC"
                     /locus_tag="ACHKW0_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HFO7475132.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(13082..13648)
                     /gene="yecM"
                     /locus_tag="ACHKW0_002306"
     CDS             complement(13082..13648)
                     /gene="yecM"
                     /locus_tag="ACHKW0_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HFO7475133.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            13864..15597
                     /gene="argS"
                     /locus_tag="ACHKW0_002307"
     CDS             13864..15597
                     /gene="argS"
                     /locus_tag="ACHKW0_002307"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HFO7475134.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYESLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            complement(15639..15683)
                     /gene="yecV"
                     /locus_tag="ACHKW0_002308"
     CDS             complement(15639..15683)
                     /gene="yecV"
                     /locus_tag="ACHKW0_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecV"
                     /protein_id="HFO7475135.1"
                     /translation="MSIFRIHLDGNKKA"
     gene            15774..16262
                     /gene="yecT"
                     /locus_tag="ACHKW0_002309"
     CDS             15774..16262
                     /gene="yecT"
                     /locus_tag="ACHKW0_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HFO7475136.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
     gene            complement(16382..16774)
                     /gene="flhe"
                     /locus_tag="ACHKW0_002310"
     CDS             complement(16382..16774)
                     /gene="flhe"
                     /locus_tag="ACHKW0_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HFO7475137.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(16774..18852)
                     /gene="flhA"
                     /locus_tag="ACHKW0_002311"
     CDS             complement(16774..18852)
                     /gene="flhA"
                     /locus_tag="ACHKW0_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HFO7475138.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PVEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLESLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(18845..19993)
                     /gene="flhB"
                     /locus_tag="ACHKW0_002312"
     CDS             complement(18845..19993)
                     /gene="flhB"
                     /locus_tag="ACHKW0_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhB"
                     /protein_id="HFO7475139.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(20195..20839)
                     /gene="cheZ"
                     /locus_tag="ACHKW0_002313"
     CDS             complement(20195..20839)
                     /gene="cheZ"
                     /locus_tag="ACHKW0_002313"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0050920 - regulation of chemotaxis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HFO7475140.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(20850..21239)
                     /gene="cheY"
                     /locus_tag="ACHKW0_002314"
     CDS             complement(20850..21239)
                     /gene="cheY"
                     /locus_tag="ACHKW0_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HFO7475141.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(21254..22303)
                     /gene="cheB"
                     /locus_tag="ACHKW0_002315"
     CDS             complement(21254..22303)
                     /gene="cheB"
                     /locus_tag="ACHKW0_002315"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HFO7475142.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(22306..23166)
                     /gene="cheR"
                     /locus_tag="ACHKW0_002316"
     CDS             complement(22306..23166)
                     /gene="cheR"
                     /locus_tag="ACHKW0_002316"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HFO7475143.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLEKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(23185..24786)
                     /gene="tap"
                     /locus_tag="ACHKW0_002317"
     CDS             complement(23185..24786)
                     /gene="tap"
                     /locus_tag="ACHKW0_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HFO7475144.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(24832..26493)
                     /gene="tar"
                     /locus_tag="ACHKW0_002318"
     CDS             complement(24832..26493)
                     /gene="tar"
                     /locus_tag="ACHKW0_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HFO7475145.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYHTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVSRVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSTFRLAASPLTNKPQTPSRPASEQPPTQPRLRIAEQDPNWETF"
     gene            complement(26638..27141)
                     /gene="cheW"
                     /locus_tag="ACHKW0_002319"
     CDS             complement(26638..27141)
                     /gene="cheW"
                     /locus_tag="ACHKW0_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HFO7475146.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(27162..29126)
                     /gene="cheA"
                     /locus_tag="ACHKW0_002320"
     CDS             complement(27162..29126)
                     /gene="cheA"
                     /locus_tag="ACHKW0_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010283911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HFO7475147.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(29131..30057)
                     /gene="motB"
                     /locus_tag="ACHKW0_002321"
     CDS             complement(29131..30057)
                     /gene="motB"
                     /locus_tag="ACHKW0_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HFO7475148.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(30054..30941)
                     /gene="motA"
                     /locus_tag="ACHKW0_002322"
     CDS             complement(30054..30941)
                     /gene="motA"
                     /locus_tag="ACHKW0_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HFO7475149.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(31068..31646)
                     /gene="flhC"
                     /locus_tag="ACHKW0_002323"
     CDS             complement(31068..31646)
                     /gene="flhC"
                     /locus_tag="ACHKW0_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HFO7475150.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(31649..31999)
                     /gene="flhD"
                     /locus_tag="ACHKW0_002324"
     CDS             complement(31649..31999)
                     /gene="flhD"
                     /locus_tag="ACHKW0_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:1902208 - regulation of bacterial-type
                     flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HFO7475151.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         7716 a         8815 c         7879 g         8136 t
ORIGIN      
        1 aaatttttta tgctattacg tatattcata tctatttcaa tggaatgaca acgtgaatat
       61 taattaccct gctgaatatg aaattggaga tatcgtcttt acatgtatag gtgctgcctt
      121 atttggtcaa atatcagctg catcaaattg ctggagtaat cacgtcggga tcattatcgg
      181 tcataacggt gaagacttcc tggttgcaga aagccgggtt cccctttcaa ccatcaccac
      241 gctatctcgt tttattaaac gttcgtctaa tcaacgctat gctataaagc gattagacgc
      301 tgggctgaca gaacaacaaa aacaacgaat tgttgaacag gttccctccc ggctacgcaa
      361 actttaccac accggtttta aatacgaatc ttcgcgccag ttctgttcaa aatttgtttt
      421 tgatatttat aaagaagcgc tatgcattcc ggtgggtgaa atagaaacgt ttggagaact
      481 gttaaatagc aatccgaatg caaaactcac tttctgaaaa ttctggttct taggttctat
      541 tccgtgggag cgtaaaaccg tcacgccagc cagtttgtgg catcatccgg gtttggtgtt
      601 gattcacgcg gagggagttg aaacgcctca gcctgaactg accgaggcgg tataacttaa
      661 cgcagtcgcc ctctcgccag gttcagccgc gattcgctca tctgcatcgc attctgacta
      721 acgtggcagt gggtgatggc aatcgccagt gcatcggcgg catccgcttg tggattagcg
      781 ggcagtttca gcaaggtgcg gaccatatgc tgcacctggc ttttctcggc gctaccaatg
      841 cctaccaccg tttgctttac ctgacgtgcc gcgtattcaa atactggcaa ctcctgattc
      901 accgccgcca caatcgccac gccgcgcgcc tggcccagtt tcagggctga gtcagcgttc
      961 ttcgccataa agacttgttc aatggcgaaa taatcaggct ggaactgggt gatgatttcc
     1021 gtcacgcccg catagatgag cttcagacga gacggtaaat catccacttt ggtgcgtatg
     1081 catccgctac ccaggtagga cagttgcctg cctacctggc gaattacgcc atagccggtc
     1141 acgcgcgaac ccggatcaat gccgagaata atagccatca cgcgtctccg ttttgctgtt
     1201 tagcaggcct catcagagag tcgctgcgac ctcatcagag atttcaccgt tatggtaaac
     1261 ttcctgcacg tcgtcgcaat cttccagcat atcgatcaga cgcatcagtt tcggtgcggt
     1321 ttctgcatcc atatcagctt tggtggacgg gatcatggaa acttccgcgc tgtctgcttt
     1381 cagacctgcc gcttccagag cgtcgcgcac tttacccatt tcttcccatg cagtgtagac
     1441 atcaatcgcg ccgtcatcat aggtcacaac gtcttcagca cctgcttcca gagctgcttc
     1501 catgatggtg tcttcatcgc ctttctcgaa ggagatcacg ccttttttgc tgaacagata
     1561 ggctacggaa ccatctgtac cgaggttacc gccacatttg ctgaatgcat gacgcacttc
     1621 agcaacggta cggttgcggt tgtcagacag acattcaatc atgattgccg tgccgccagg
     1681 accgtaacct tcgtagatga tggtttccat gtttgcatca tcatcaccgc ccacaccacg
     1741 tgcaattgcg cggttcagtg tgtcacgggt catgttgtta gacagtgctt tatccaccgc
     1801 cgcacgcaga cgcgggttag cgtccggatc gccaccgccc agcttagccg cggttaccag
     1861 ctcacgaatg attttagtga agattttacc gcgcttagca tcctgcgcag ctttacgatg
     1921 tctggtgttg gcccatttac tatgacctgc cataaaaata tctccagata gccctgcctg
     1981 ttcaggcagc gttaattaca aactgttcaa tcgcctgccg gttgctccag gacttagtga
     2041 gcgccgccgc agcaggcgca tcaagccact tgtaagccag atgttcagtg aaaacgatct
     2101 gccgctcgtg cggaagcgca agacagaacc atgattccgt attacgcgtc acgcccggcg
     2161 catagcgatg acgtaaatgt gaaaaaattt caaactctac cgtgcgctga cagtcaatta
     2221 aggtcagttg ttcagcgaca acatcaatgg tgacctcttc ctttacttcg cgcatggcag
     2281 cttgcggcgc ggtttcaccc tcttccacgc tgccggttac cgactgccag aaatcgggat
     2341 cgtcacgccg ctgcaacatc agcacccgtt tcgtatcttg tgcatagatg accactaaga
     2401 tcgaaacggg acgcttatac actttatcct ccacgctgcc tcttcgttga ctgccttcgc
     2461 tcatcccatt cacatagtta tctatgctca tgggagttca ctcagttgcc gccttgatgc
     2521 aacgcgaatg atttcgtgta tttgagtcat atcagttatt ctcagccttc ttcacaacct
     2581 gaatgctcag ctcagccagt gcagtcgggt tagcaaagct cggtgcttca gtcatcagac
     2641 acgctgccgc cgtggttttc gggaaggcga taacgtcacg gatattgtcg gtgccggtca
     2701 gcagcatggt cagacggtca agaccgaatg ccagacctgc gtgcggcgga gtaccgtatt
     2761 tcagagcgtc gagcaggaag ccgaatttct cgcgctgttc ctcttcgttg atacccagaa
     2821 taccaaacac cgtctgctgc atatcaccat tatggatacg tactgaacca ccgcccactt
     2881 cgtaaccatt gatgaccata tcgtaagcgt tcgccaccgc attttccggt gcagctttca
     2941 gttctgcagc agtcatgtct ttcggtgagg tgaacggatg gtgcattgcc gtcaggccgc
     3001 cttcaccgtc gtcttcaaac atcgggaagt cgataaccca cagcggtgcc catttgcttt
     3061 cgtcggtcag accaaggtct ttacccactt tcaggcgcag tgcgcccatc gcgtcggcaa
     3121 caattttctt gttgtcggca ccgaagaaaa tcatatcgcc atcttgcgcg gcagtacgat
     3181 ccaggatggc ttcgatgatt tctgcattaa ggaacttcgc taccgggctg ttgatacctt
     3241 ccagaccttt cgcgcgttcg ttaactttga tgtaagccag acctttcgcg ccgtagattt
     3301 taacgaagtt accgtattcg tcgatctgct tacgggtcag cgatgcgccg cccggaacgc
     3361 gcagagcggc aacgcgacct ttcggatcgt tcgccggacc tgcaaatact gcaaactcaa
     3421 cagatttcag cagatcagca acgtcggtca gttccatcgg gttacgcaga tccggtttat
     3481 cagaaccata acggcgttct gcttccgcaa aggtcattac cgggaaatcg cccagatcca
     3541 cgcccttcac ttccagccac agatggcgca ccagcgcttc catcacttca cgcacttgcg
     3601 gcgcggtcat gaaagaagtt tccacatcga tctgagtaaa ttcaggctga cgatcagcgc
     3661 gcaggtcttc gtcacggaag catttaacga tctgatagta gcggtcaaag ccggacatca
     3721 tcagcagctg tttgaacaac tgcggggatt gtggcagcgc gtagaattta cctttgtgca
     3781 cacgagaagg caccaggtag tcacgcgcgc cttccggcgt ggctttggtc agcatcggag
     3841 tttcgatgtc gaggaagccg tggtcatcca taaaacggcg caccaggctg gtgattttag
     3901 cgcgggtttt caggcgctga gccatttccg ggcgacgcag gtcgaggtag cggtatttca
     3961 gacgcgcttc ttcggtgttg acgtggttag agtcaagcgg cagaacatct gcgcggttga
     4021 tgatagtcag cgaggacgcc agcacttcga tttcgccggt cgccatatcg cggttaatat
     4081 ttttttcgtc acgcgcacgt acggtgcccg tgacctgaat gcagaactca ttacgcagtt
     4141 cagaggccag ctttaacgcg tccgcacgat ccggatcgaa aaatacctgc acgatacctt
     4201 cgcggtcgcg catatcgatg aagatcaggc taccaagatc acgacgacgg ttgacccaac
     4261 cacacagagt cacctgctgc cccacgtggg acaaacggag ctgtccacaa tattctgtac
     4321 gcatgagata tcccttaact tagctgccgg cggatgcccc ctgctgcgca ggtgaccaag
     4381 tcgcagcgtt agctgtatgt cacaactgaa tgaaaaaagg cggctattat actggaaatt
     4441 ctgccgcacc gtaagagcct ggcccgcgct ggaacgcctc gttaccactt tatatcgggc
     4501 ctgaaatcag actctacgcc agtttgctat aaaggtgttg cccgaactca taaaaattaa
     4561 caaaatttgt cgttccgcca tcggctaatc gcattaaggt gtgaggcacg attttgtttt
     4621 ttcaggagtc attatgcttg aacttaatgc taaaaccact gcgctggtgg tgatcgattt
     4681 acaagaaggc atcttacctt ttgccggtgg tccacatact gccgatgagg tagttaatcg
     4741 cgccgggaag ctggcggcga aatttcgcgc cagcggtcag cccgtgtttc tggtgcgcgt
     4801 tggctggtct gccgattacg ccgaagcatt aaaacagccg gttgatgccc cctcccccgc
     4861 taaagtgttg cccgaaaact ggtggcaaca tccagctgca ttaggtgcaa ctgacagtga
     4921 tatcgaaatc atcaaacgtc agtggggtgc gttttacggt acggatctgg agttgcaact
     4981 gcgccgccga ggtatcgata caatagtgtt atgtgggatc tcgaccaata tcggtgttga
     5041 atccaccgcc cgcaatgcct gggaacttgg ctttaatctg gtgattgccg aagacgcctg
     5101 tagtgccgct agcgccgagc agcacaataa cagcatcaac catatctacc cacgcatcgc
     5161 ccgtgtgcgt agcgtggaag agatccttca cgcgttatga tttacatcgg tctaccgcaa
     5221 tggtcgcatc ctaaatgggt gcggttgggg atcaccagcc ttgaagagta tgcccgccac
     5281 tttaactgcg tggagggcaa caccacgctt tacgccctgc cgaaacccga ggttgtcctg
     5341 cgctggcgtg aacagaccac agatgacttc cgcttctgtt ttaagtttcc ggcgaccatt
     5401 tcgcatcagg cagcattacg acattgcgat gatttagtga ctgaattttt gacccgcatg
     5461 tcaccgttgg ctccgcgcat tgggcaatac tggctgcaac tgcctgccac attcggccca
     5521 cgggagctgc ctgcgctttg gcattttctc gattctctcc ctggcgaatt taattatggt
     5581 gtggaagtcc gccatccaca gtttttcgcc aaaggggaag aggaacaaac tcttaatcgc
     5641 ggtttacatc agcgcggcgt taatcgggtg attttagacg gccgcccggt tcatgcagca
     5701 tgtccacaca gtgaagctat tcgcgacgct caacgaaaaa aacctaaagt tccggtacat
     5761 gctgtactga cggcgacaaa tccgctgatc cgttttatcg gtagtgatga tatgacgcaa
     5821 aaccgggaat tatttcaggt ctggttacag aaattagcgc agtggcatca gaccactacg
     5881 ccttatcttt ttttacatac gccagacatt gcccaggccc cggaactggt acataccctg
     5941 tgggaagact tacgtaaaac gcttccagag atcggagcag ttccggctat tccacagcaa
     6001 tcttctcttt tctgaatttg ccacctatca tagacaggtg ccatcggcca ttttaaaggg
     6061 agtttgtatg gtaagcgcgc tatatgccgt tttaagtgcg ttgttattaa tgaagttctc
     6121 ttttgatgtc gttcgcttgc gtttgcagta ccgcgttgcc tatggtgacg gtggttttag
     6181 cgaactgcaa agcgctattc gcattcatgg taacgcggtg gaatatattc ccatcgcgat
     6241 tgtgctgatg ctgtttatgg aaatgaatgg cgcagaaacc tggatggtgc atatttgcgg
     6301 catcgttttg cttgctggtc gtctgatgca ttattacggt tttcatcacc gtctgtttcg
     6361 ctggcgacgt tccggcatga gcgccacctg gtgtgcgctg ttgctgatgg tgctggcgaa
     6421 tctttggtat atgccctggg agttggtttt ctccctgcgt tagcgcacaa tacgccactt
     6481 tctttttccc ggatttttac gttatgtctc accgcgacac gctattttct gcccctatcg
     6541 ccagactggg cgactggacc tttgatgaac gggtagctga agtcttcccg gatatgatcc
     6601 agcgttccgt tcccggctat tccaatatta tttccatgat tggtatgtta gccgagcgct
     6661 tcgttcaacc tggtacgcag gtttacgatc tgggttgttc tctgggcgcg gcgacgctct
     6721 cggtgcgtcg caacattcat catgataatt gcaaaattat tgccatcgac aactccccgg
     6781 cgatgattga acgctgccgt cgtcatattg acgcctataa agcccctacg ccagtagacg
     6841 tcattgaagg tgatattcgc gatatcgcca ttgaaaacgc atcgatggtg gtgctgaatt
     6901 ttaccctgca attcctggaa ccttccgagc gccaggcatt actggataaa atttatcaag
     6961 gactgaaccc aggcggcgcg ctggtgcttt cggaaaaatt cagtttcgaa gatgccaaag
     7021 ttggtgaact gttgttcaac atgcaccacg actttaaacg tgccaacggt tacagcgaac
     7081 tggagatcag ccagaagcgc agcatgctgg aaaacgtgat gctgaccgat tccgtggaaa
     7141 cccataaagc acgcctgcat aaagccggtt ttgagcatag cgagctgtgg ttccagtgct
     7201 ttaactttgg ttcactggtg gcattaaaag cagaggacgc tgcatgatcg actttggtaa
     7261 cttttattct ctgattgcca aaaatcatct ttcacactgg ctcgaaacgc tgcccgcgca
     7321 gattgctaac tggcagcgcg agcagcagca cgggctgttt aagcagtggt ccaacgcggt
     7381 ggaatttctg cctgaaatta aaccgtatcg tctggattta ttgcatagcg taaccgccga
     7441 aagcgaagag ccactgagca ccgggcaaat taaacgcatt gaaacgctta tgcggaacct
     7501 gatgccatgg cgcaaagggc cgttctcact gtatggcgtc aacatcgata ccgaatggcg
     7561 ttccgactgg aaatgggatc gtgttctgcc ccatctttct gatttaaccg gacgcaccat
     7621 tctggatgtc ggctgcggca gcggttatca catgtggcgc atgattggcg caggcgcgca
     7681 cctcgcggtg gggatcgacc ctacgcaact gtttctgtgc cagtttgaag cggtgcgtaa
     7741 actactgggt aacgatcagc gcgcacattt gttaccgtta ggtattgaac agcttccggc
     7801 actgaaagcc tttgataccg tcttttcgat gggcgtgctt taccatcgcc gttcgccgct
     7861 ggagcatctc tggcagttga aagatcaact ggtgaatgag ggtgaactgg tgctggaaac
     7921 gctggttatt gatggcgatg agaacacggt gctggtacca ggcgatcgtt acgcgcaaat
     7981 gcgtaatgtc tatttcatcc cttccgcgct ggcgctgaaa aactggctga agaagtgtgg
     8041 ttttgtagat atccgcgttg tggatgtgtg cgttaccact actgaagagc agcgacgcac
     8101 tgaatggatg gttaccgaat ctctgtccga tttcctcgac ccacatgatc cgagtaagac
     8161 agttgaaggt tatcctgcgc ctaaacgcgc ggtgctgatt gcgcgcaagc cgtaaaggtc
     8221 tggcattact gccggatgcg gcttgaatgc cttatccggc ctacaaaccc ttgctaattc
     8281 aatctattgc agggactatg taggcctgat aagcatagcg catcaggcag ttttacgttt
     8341 gcataacctc agcgcccgtt tccgggcgct attcacttct tatgcctgaa tagctggctg
     8401 atcaaacgcc gttaacttcg gcgcattgcc cgtatatttt tcgatataca ccagcgcaga
     8461 gttaccggca caggcatttg ccagctgcga gctggggata tccgccgtta acacgttcgc
     8521 actgccgttt ttacacaagc cattttccag atctggccat gcaccttcat gcacgcatac
     8581 cacgcctttt ttgatcccgt cagtcaccac cgcgcctgtc agaatctgac cacgtttgtt
     8641 ccacacacgc accagatcac cattcgcaat accaaagcga gcagcatctt cggtgtgaat
     8701 agtgattggt tcacgatctg caaccgcata ttttttacgc agttccgcat agttaagctg
     8761 actatgtaaa cggtgtgccg gatgtgcggt cagaagctgc aactgcttct cgtcggcggt
     8821 acccttccac tcatcaggcg caagccaggt tgggtgtgcc gggcaatcct tatagccaaa
     8881 tttttccagc gttttggagt aaatctcaat tttgccgctt ggcgtaccca gcgcattttt
     8941 caccggatcg gcgcggaaat cagcataacg aacgtactgt tcgttcttct cgctgcggcg
     9001 catttcgatc agtttatttt gctgccagaa ggcattaaac atcggcatag tcacgcgttg
     9061 cgcacgagca cctttctgag cggcatcata gaaaaatttc agccacgcca tttcatcttt
     9121 accttcggta tagatctctt ttccgccagg tttcagtaat tcagcaagat cggcaaatac
     9181 gtcaaaatcg ttacgcgctt caaattgcgg agcaacagcc tgcttcatcg gcacaatatg
     9241 ctggttgctg taatcaccgg tcatcgtcaa gtcattgcgc tcaaacgatg tggtgatcgg
     9301 taatacgata tctgcatgtt tagcagctgc ggtccagtag cattcagaaa cgacgatcat
     9361 ctccggtttc tgccatgctt tgatcagacg attagtatcc tggtgatggg taaagttacc
     9421 gccacccgcc caccagatca ttttgatatt cggatacgtt tgttctttac cgttatgctg
     9481 atatttgcca ccaggatttt ccagcgcatc gacgatacgg gcaacaggaa tcgccgtcat
     9541 tccgccatca tcagcggcct cgctggcctg tccggcaatc gccgcagaca tttccggcag
     9601 cacgccgcca acacgcgtcg gattgccacc gttggagtaa tgataagaga aaccaaaacc
     9661 gccgcccggt gtaccaatct gccctaacat cgccgccagc gtcaccagca tccagtgttt
     9721 ctgttcacca tattgctggc gctgaattcc ccagccagcc atcagcattg tgcgattcgc
     9781 cgccatcagt tcagcgagtt tgactatctg cgcttccggt acgccagtaa tttccgccgc
     9841 ccaggccgcg ctcttcggag tattatcgct cttacctgtc agatactctt caaactgcgg
     9901 atacccggtg gtgtattttt caaggaacac tttatcgtgt ttaccttgtg tcatcagggt
     9961 atgcgcaatc cctaacatca gtgccacgtc ggtgcccata ttcggcgcga tccaggtagc
    10021 attatcacca aagaactcga tggtttcgga gcggatagga tcaatggcaa tcactggttt
    10081 gccagatttt ttcagctgat ggaagtattc cagcccttgc tcatcggtac tgctccaggc
    10141 aatttttaag gtattcagcg ggttcatccc ccacagcacc acagcctggc tgttttccag
    10201 aatcagcggc caggaggtct gctgttcata cacctctaca gaaccgacca catgcggcat
    10261 gatcacctgt gcagccccgg ttgaataatc gccgctatgc ccggaataac cgcccgccag
    10321 gttcatataa cgttgcagta aggtttgcgc tttatgcaac acgccagaag agcgccagcc
    10381 gtatgatccg gcaaaaatag cagatggtcc gttagctttg cgaatgcgat cgtgttgctc
    10441 atgaatcagt tttaatgctt gctcccagct cacctgtaca taagtatctt cgccacgacc
    10501 tttcgccggt tgcagtggat tatcgagata gctttttctt accatcggat gctgaatacg
    10561 tgccgtggtg tgtacctgat ccgccgccgt agactgtaag gagttcggta tggttttcgc
    10621 cagcgcgcct gttgaagaaa caatcttgcc gtccttcact tctacgttca tcgctcccca
    10681 acgtcccgcg gtgaggattt taccgccctt ctcttctgcc catgcgggga gcggtgctgc
    10741 cgatgtcacc accagcgctc cagcggcaat accgctgtgt ttaataaatt cacgtcttgt
    10801 taatgtcata acttcctccc tgatcaacga ggatcactgt ttctcggtaa tatctttggc
    10861 gttgtactgg aaataccgcg ttaaaatgtc cagttcgttt tcgctcatgc tggttcgcgc
    10921 acccattcct ttggcaatgg acggccatgc attgacggtg taatggtcag cggcaatagg
    10981 cgcatggcaa ccagcgcaat aggtatcgtc aagtttttca gcgtattgcc atagcggttt
    11041 acggtccgct aatgcgggat cggtaagcgc accctgtaaa gacgcctgac gccattgatt
    11101 gccgtattcg tcagcctgcc attccccgtt taccgtgagc gccttgatac cttcttcact
    11161 taatgtggct agcgccagcc gttgacctgc cgccaggtag agcgtgtttt cactgccctg
    11221 catttgataa ccctgcaaca gaacgatcgg ctgtttgcca ctggcatcaa cgacggtgag
    11281 atcggtacca ggattcacgg tagccagctc gcctatgcga gaagttttga aaggataaat
    11341 atgtgcgcca ttagtcactg aagtagcggc ctgactttcc agctcatgcg ccgcgttgtc
    11401 atccattttt atttctggcg gaaaatgggc aatgccttta tgacaatcga tacaggtttc
    11461 gccgcccttt tgtgctttgt tatgcatttt ctgcgcagat tcactttgcg aggcaatatc
    11521 catggcatca aaagaatggc aactacggca cgttgcagag tcagttgctt ttaattcttt
    11581 ccatactgtt tcggccattt cctggcgatg agtttcgaac ttatcgtcac tgtctatttt
    11641 gccgctaaca aattcatgat aaatatcttt agatgctttt aatttagcaa ataaataatc
    11701 catccctgac tttggaatat ggcaatctgc acattccgca cgtatccctt tctggttcga
    11761 aaagtggaca gttccctgat attcctcaaa aggtttactc atcgagtggc aggaaaggca
    11821 aaatgctgta tccgacgttt tatgtaaggc tttttgcgcc agcaataacc cgccgccacc
    11881 aaccacaacc gctatcagca aaaacaataa cccaatgcgt tttttccctc gcatagattc
    11941 ctctaaataa attccagaac accgaaatca ttgtataatt aaatacacaa cgaattaacc
    12001 gcttgcataa ctcggcacaa cactgcctga aataatcgat aaagaatatg atttattaca
    12061 atgtaatcat taattgctaa ggaataaccc agttgctatt tagaatatat atttatcagt
    12121 tttagcaatt taaatcccat aatcaatgtg aatataaaaa atattattag cacaacgcta
    12181 tgttcactgt cacccgctca acagatcacg gttatcgttc gttttttata ctgctcaggg
    12241 ataaaaaaag gcccctgttg aaattgcagg ggcctggtac gagcaagcat catattgggc
    12301 gacatgatgc aacggtaaaa atcatttggc ctgatggcgt tcaatgattc ctttcatttc
    12361 agcaaccgcc gccccgtcta cgatataacg cgaatactcg tccgcgtgtt catctgatga
    12421 catggacaat ccttgattac gataacgcat cggtgaggct tgccacgctc ccgcggagct
    12481 atggacttcc agcactccgg catcgaggaa gtggtgcagg ttttctgcac ggactcctgc
    12541 tccggccata atgattggag catcacgatg ggcaataagt tccataattt ttgataaacc
    12601 ttgcagtgcg tctgattttt gccctgatgt cagtacccgg gcaatgccca attccgcaag
    12661 attattgagt gtatataaag ggttagcgca catatcgaag gcgcgatgaa aagtcactgc
    12721 cagcggaccg gcagcagcca ttattttttc cattcgtggc atatcgacat tcccgtcaac
    12781 atcgagaacg cccgtcacca gtccaggaaa acctaattcg cggaccgtgc gcacatcctc
    12841 aagaatggcg gcaaactcac cgtcgctgta gcaaaaatca ccaccgcgtg ggcgaattat
    12901 cggatgcaca gggatcgtca cccgctggcg cacggatttc agtaccccca acgacggcgt
    12961 taagcccccc tcttttgggg ctgcgcataa ttcaactctg tctgcgccgt tttgctgcgc
    13021 cgttagtgca cattccatgc tgtaacagca aatttccagt aatgccattt ttgctcctta
    13081 attacgctga ctgctcgctg gcaacgatct cttcaatgga ccacggatga aacttaatag
    13141 ttgttttgcc atcggtgacc gccagcgtcg gattcggtaa tcgctcatgc tcaccttttg
    13201 gtgaactggt tttaactgag atccccggta ggctgagtcc ttcatcagaa agcaatgcca
    13261 gcgcacgggc attcagcgtt tctggatcac ccggcagaac gatttcaata tgttcccaac
    13321 cttcgtgtgg gtaacgtttt tccccgggcc acggtagctc cacaatagaa aactgccagt
    13381 gcgcaacctg taccggttca tgcaatttaa acagacaaat cggtctgcca ttgatcatat
    13441 tttctgacaa aagctcgcca cactgttcaa acccgcgacg ccagcgttca gcagtggcgt
    13501 tttgatggca acgcaaagaa atgtgatcgg cagtcagcgg agtgatattc aaccccagac
    13561 ggagggaaag ttcatctaat gcgtgggtaa atcgcggtaa atccgatgca atatcctgca
    13621 gctcgtcgat agattgccag ttcgccataa tcactcttcg tctttcagta aaagcgttaa
    13681 tttaccctgt tgccctgcgc caaccaaccg ctgatttcac gccgcttctg atgcaatagt
    13741 gaaaacggca atacgccacg cgcacgttgc tgacgaaaac agccatttgc agtatactcc
    13801 cgccctaatt tctttaactg gtgcgggcaa tttttgctcg cttcatcaat gtaaggtatt
    13861 ccggtgaata ttcaggctct tctctcagaa aaagtccgtc aggccatgat tgcggcaggc
    13921 gcgcctgcgg attgcgaacc gcaggttcgt cagtcagcaa aagttcagtt cggcgactat
    13981 caggctaacg gcatgatggc agttgctaaa aaactgggta tggcaccgcg acaattagca
    14041 gagcaggtgc tgactcatct ggatcttaac ggtatcgcca gcaaagttga gatcgccggt
    14101 ccaggcttta tcaacatttt ccttgatccg gcattcctgg ctgaacatgt tcagcaggcg
    14161 ctggcgtccg atcgtctcgg tgtttctacg ccagaaaaac agaccattgt ggttgactac
    14221 tctgcgccaa acgtggcgaa agagatgcat gtcggtcacc tgcgctctac cattattggt
    14281 gacgcagcag tgcgtactct ggagttcctc ggtcacaaag tgattcgcgc aaaccacgtc
    14341 ggcgactggg gcactcagtt cggtatgctg attgcatggc tggaaaagca gcagcaggaa
    14401 aacgccggtg aaatggagct ggctgacctt gaaggtttct accgcgatgc gaaaaagcat
    14461 tacgacgaag atgaagagtt cgccgagcgt gcacgtaact acgtggtaaa actgcaaagc
    14521 ggtgacgaat atttccgcga gatgtggcgc aaactggtcg acatcaccat gacgcagaac
    14581 cagatcactt acgatcgtct caacgtgacg ctgacccgtg atgacgtgat gggcgaaagc
    14641 ctctacaacc caatgctgcc aggaattgtg gcggatctca aagccaaagg tctggcagta
    14701 gaaagcgaag gggcgaccgt cgtattcctt gatgagttta aaaacaagga aggcgaaccg
    14761 atgggcgtga tcattcagaa gaaagatggc ggctatctct acaccaccac tgatatcgcc
    14821 tgtgcgaaat atcgttatga atcactgcat gccgatcgcg tgctgtatta catcgactcc
    14881 cgtcagcatc aacacctaat gcaggcatgg gcgatcgtcc gtaaagcagg ctatgtaccg
    14941 gaatccgtac cgctggaaca ccacatgttc ggcatgatgc tgggtaaaga cggcaaaccg
    15001 ttcaaaaccc gcgcgggtgg tacagtgaaa ctggccgatc tgctggatga agccctggaa
    15061 cgtgcacgcc gtctggtggc agaaaagaac ccggatatgc cagctgacga gctggaaaaa
    15121 ctggctaacg cggttggtat tggtgcggtg aaatatgcgg atctctccaa aaaccgcacc
    15181 acggactaca tcttcgactg ggacaacatg ctggcgtttg agggtaatac cgcgccatac
    15241 atgcagtatg cttacacgcg tgtattgtcc gtgttccgta aggcagaaat taacgaagag
    15301 caactggctg cagctccggt aatcatccgt gaagatcgtg aagcgcaact ggcagctcgc
    15361 ctgctgcagt ttgaagaaac cctcaccgtg gttgcccgtg aaggcacgcc gcatgtgatg
    15421 tgtgcttacc tgtacgatct ggctggtctg ttctctggct tctacgagca ctgcccgatc
    15481 ctcagcgcag aaaacgaaga agtgcgtaac agccgtctga aactggcaca actgacagcg
    15541 aagacgctaa agctgggtct ggatacgctg ggtattgaga cagtagagcg tatgtaatcg
    15601 atttttcgtg agagtaaagc ctgatcaggg ttacctgatc aggctttttt attgccatct
    15661 aaatgtattc tgaaaatgga catgccattg ttttctgact gttggataag aggccagaag
    15721 cgtaatatcc ggccccaggg aaacgataac ggttgaattt aaggaatacc gcagtgttta
    15781 aatttcttgt attaacatta ggcattatct cttgccaggc ttacgcagaa gatacggtta
    15841 tagtaaacga ccatgacatt tcagccatca aagattgttg gcaaaaaaat tcagatgatg
    15901 atactgacat taacgtgatc aaatcatgcc tgcgacaaga atacaatctc gtcgatgcgc
    15961 aattaaataa agcctatggt gaagcttatc gttatataga acaagtgcca cgcacaggtg
    16021 taaaaaaacc tgataccgaa caacttaact tgcttaaaaa atcacagcga gcctggctgg
    16081 attttaggga caaagaatgt gaattaatcc tttcaaatga ggacgttcag gatttaagtg
    16141 acccttattc tgaatcagaa tggctctcat gtatgatcat acagaccaat acgcgaactc
    16201 gccagttgca gctataccgt aactctgaag atttttatcc aagccctttg acaagaggat
    16261 aattcacatc tttttggcat gttttgttgc aagctattcc tgataaataa ttgcaacaag
    16321 acatcgagcc tttttcactg agttattaaa catactcgcg agcgcgtaat ttttttgtcc
    16381 ttcagcgata attcacaatc acttcattac gttgtacctt tagcggtgga attaaccgcc
    16441 caccgcctgg cacttcccag ataaatcgca acggttctgc tgccgctata ccggaaaagg
    16501 ccacggtggt tccgctctgc ccctctaatt cgacacaacg agattgcgaa cacaagcgaa
    16561 cccgcagtcc tgaaggtgtc gggccgataa gctgataacg ccacgctacc agcgtcatca
    16621 atcctgaagc aggttgtcgc gtagaaagag gcgcagacga catcgactca ccgcgatgat
    16681 ttagcgtaat tcctacacta cttgcctgcc acatcccctc cccggcggct tgcaccagca
    16741 gcggaaacaa taatattgct aataaggttc tcattatttg ccgccaattg tcgccgtcat
    16801 gcggatatgt cggttatcag acagttccag attcgacagg accactaact gcggcaagct
    16861 gcggcgcagg aagcgagaca ataatggtcg cagcgcgtgg ttcaccaaca atactggcgg
    16921 cgcacccagc atctcctgac gggatagcgc ttcctgagtt tgcgccagta aacgatccgc
    16981 cagccctggc tccagtcctc ccccgccctg cagcgcctgt agtaacaaac tttccagcgg
    17041 tgtatcgagg ccaataacat ggacttcatc tttgccagga aaccactgct gggtaatcgc
    17101 ccgtcccaac gccacgcgca cgacggcggt taattcatgt ggatcgcttt ggatgggcgc
    17161 atgttccgcc agcgtttcga gaatggtgcg catatcgcga atcggtactt tttcatcgag
    17221 gagattttgc agcactttat gcagtgtggt gagcgtgacg acgccaggaa cgagatcttc
    17281 cgtcagcttt ggcatctcct gggcgacgcg atccaacagc tgttgcgcct cctgacgacc
    17341 aaacagctct gcggcatgct ggctaatgag gtggttaaga tgcgttgcta ccaccgtgct
    17401 ggcctcaacc actgtgtacc cctgaatctg cgcctgttct tttagcgcac tttcaatcca
    17461 gatagcattc aggccaaatg ccggatcgac ggtcgcctca ccaggtaacg tcccggcagc
    17521 ggttccaggg ttaatcgcca gccagcgccc cggataagca tcaccactgc caatctccac
    17581 gcctttcatc aaaatgcgat agcgggcagg ttgcagatcc atattgtcgc gaatgtgcac
    17641 cactggcggc agaaatccca tctcctgggc aaatttcttg cggatactgc gtatacggcc
    17701 caacaactca ccatcctgct ggaaatcgac catcgggatc agtcgataac ccacttccat
    17761 tcccagagaa tcttccagtt gtacatcgtt ccacgtcgct tcgacaacgg tattattctc
    17821 tgccattttt accggtttgg gttcgacagg cgctttttgt tcgcgtccgc gtatccacca
    17881 ggccagcccc agcaatccgg cagtgaacag caaaaatacc aggttcggca ttccaggcac
    17941 caggccgagt aaaccgagca cggcggcgct taacaacata acgcttgggt tactgaaaag
    18001 ctgattcacc atctgctcgc caacatcctg atcggtgctg acacgcgtaa cgatgacccc
    18061 cgcggcggta gaaatcacca gcgccggaat ttgtgccacc agaccgtcgc caatggtcaa
    18121 tagtgtataa ctttccgccg cgtgtcccat gctcatgcca tgttgcagca cgccgaccag
    18181 caacccgccg acaacgttaa tgaccatgat gaggatcccg gcgatggcat cgccgcgaac
    18241 aaacttactt gccccgtcca ttgagccgta aaaatcggct tcctgagtca cttcggagcg
    18301 gcgttttttc gcctcatctt caccaatcaa tccagcgtta aggtcggcgt caatcgccat
    18361 ctgcttaccc ggcataccat cgagaacaaa gcgcgcaccc acttctgcga tacgcccggc
    18421 acctttggta atgaccataa agttgatgat cacgagaatg acaaacacta cgataccgat
    18481 agcgaaattg ccaccaacga ggaagtgacc gaatgcttcg accaccttcc cagccgccgc
    18541 cgcgccggta tgcccttcca tcaaaatgat acgggttgaa gccacgttaa gtgccagacg
    18601 caacaacgta gtaaacaaca gaatggtcgg aaacgcagca aactcaagcg tgcgctgggt
    18661 aaacatcgcc accagcaaca ccatgatcga caaggcaata ttgaaggtaa acaacaggtc
    18721 gagtatgaat gcgggcagtg gcagcaccat catcgacaag atcaacagga tcaaaatcgg
    18781 tccggcaagg atctgccatt gtgtcgattt caggtttgcg ggcaggcgca gcatcgcggc
    18841 cagattactc atgggtcggt ttctcgttaa taaaatccag ggcttccggc accggaagat
    18901 gagtaggttg tacagggcgc tgtccaccag ccagacgcca gcgtttcagt tgccagaccc
    18961 aggccagcac ttccgccacc gcggcgtaca gttgacccgg gatttgttga ccaatctccg
    19021 catgtcgata cagcgctcgc gccagcggcg gcgcttcaag cgtcgggacg ttattttcag
    19081 cgccaatttc acgaatgcgc agcgcgacca gccctgcacc tttagcgacc actttcggtg
    19141 cgctcatttt gttttcgtca tactgcaacg ctaccgaata gtgggtcggg ttattgacaa
    19201 tgacatccgc tttcggcaca tcggccatca tccgacgccg tgcagcagct cgctgcatct
    19261 gacggatccg ccctttaaca tgagggtcac cttcgctttg tttgaactca tcacgaatat
    19321 cctgccgtga catacgcagc tttttcaggt ggctgaatat ttggaaaaag acgtcaaatc
    19381 ccaccattgg aatgacacca agcaccacca gcagtgcgca tagccctacc aaatccatcg
    19441 cattacccat ggcggtaatc ggagactcgg ccatcaagcg catcatctgc ggccagtgat
    19501 gccagagaaa aaaccccgtc acgctgccaa ccaggatggt tttcaaaatt gctttaagca
    19561 actccgcgcc agtctgagcc gagaacatcc gtttaatgcc cggtagcggg ttgagtttgg
    19621 aaaacttcgg ctgcaaggat ttgccgctaa ataccagccc tcccagcatg accggagaaa
    19681 tgagcgccac cagcaccacg ccgctaatca gcggcagcag cgccagcatg gcttctctga
    19741 tcagcagaat aatctgcccg aggatcagat tcgggtcatt gataatactg tgatcaaaat
    19801 gcagcccagc ggagagcatg cccgacaatc gacgggccag cgacacaccg ccaaaccaga
    19861 taacactaac gcccaccagc aaaatcagca gtgaggtcag ttcacgggaa cgcgggattt
    19921 gcccctcttc ccgcgctttt tctagtcggt gaggtgtggg ggcttctgtt ttgtcgtcgc
    19981 tctcgtcaga cacgtcgcca atcctggaaa gagtattaag cgtgaatgat gccagagcgc
    20041 aaagcgttca atggtttgag taaggggcaa aacaggcggg atttagggct tttgctgcca
    20101 cacatcaagc atagtgtgcg tttgtcggat gcggcgtcat cgccttatca gaccgcctga
    20161 tatgacgtgg tcacgccaca tcaggcaata caaatcaaaa tccaagacta tccaacaaat
    20221 cgtccacctg atcctgactg gctaccacgc cggctttgct ggtatcgacc tgaggtccat
    20281 taagcaaact ctggttttca cgttttggac gcgactcctg ttccgggatg ttttccaaca
    20341 gcaccatcag caactggcgt tcgatctcct gaatgacatc catcatccgc ttaatgacct
    20401 gcccggtgag atcctgaaaa tcctgcgcca tcatgatttc cagcagttgc gcgttagtaa
    20461 agctggtatg cgcgggtaca tctgccagaa attgtcgtgt atctgttacc agttcacggg
    20521 cgtcggcaag gtcaatcgga tcggcaaacc agtcatccca acgttgggtt aacgcttttg
    20581 ctgatttctc catttgatcc tgatgcggtt gtgacgcctc aacactgttc agcgcccgct
    20641 ccgcagcctg ggcggtcatc tgcacaacat agtacaaacg atcgcgcgca tcggggatgg
    20701 cttccgccgc ttcggcaatg gcctgatcca gccccagttc ccgcaaactg tcgcgcagca
    20761 tacgcgtcag gctgccgatg cgcgcaatga tatcgccagc tgaatgctcg tcagcaggtt
    20821 tgattgatgg ttgcatcata gtcgcatcct cacatgccca gtttctcaaa gattttgttg
    20881 agtttttcct ccagcgtcgc ggcggtaaat ggcttcacca catagccact ggcccccgct
    20941 tgcgccgcag caatgatgtt ctctttcttc gcttctgcag tcaccattaa cactggcaat
    21001 gccgacatcg cgccatccgc acgaattgtt ttcagcaatt ccaggccatc catattgggc
    21061 atgttccagt cggagataac aaatccataa ccgcctgcct gcaacttatt gagagcgtcg
    21121 acgccatctt ccgcttcctc aacattattg aatcccagct ctttaagcag gttacgcact
    21181 atgcgtcgca tggtggaaaa gtcatccaca accaaaaatt taagttcttt atccgccatt
    21241 tcacactcct gatttaaata cgtatcgcct gtccggcact aatttttgcc aacatttgct
    21301 ggcttacctg gctaagatcg accacttcgc agacaccacc catattgatg gcctcgcgcg
    21361 gcatgccgaa caccacgcaa cttgcttcgt tttgcgcaag ggtccatgcc cccgcctgac
    21421 gcatcgccaa cattcccgcc gcgccgtcgt tgcccatacc ggtcaggatc accccaaccg
    21481 cattacgccc cgcctgtttg gcgacagaat ggaacaacac atctaccgaa ggccgatgac
    21541 ggttaaccgc cgggccatcg tgaattttga tttggtaatt tgcgccacta cgcgacagct
    21601 ccatatgccg atcgcccggc gcaatatagg catgccccgg caagacacgt tctccgtctt
    21661 cggcttcttt aaccccgatc tggcaaagct tattaagtct gtcggcaaaa gagcgggtga
    21721 aaccgggcgg catatgttgg gtaattaaca atgccgggct ggaaagcggc aacggttgca
    21781 gtacgtgacg aattgcctca gttccacccg ttgaagcacc aatcgcaatc agtttttcag
    21841 aactcaacaa cggacccgcc ttcagcgttg tcggtgctga caatggctta tgtgctgcaa
    21901 ggctcgcctt tgctgccgta cgcacctttt cagcaatcat ttcgctatac gccagcattc
    21961 cttcgcgaat acccagttgc ggtttggtga caaaatctat tgcccccagc tccagcgcgc
    22021 gcagcgtgac ttctgatcct ttgccggtca aggaagaaac catcacaacg ggcattggac
    22081 gcaaacgcat taatttttcg aggaaatcca gtccgtccat ccgcggcatt tcaacatcca
    22141 gcgtcagcac atcgggattg aatttcttaa tcaagtcacg cgcgaccagc ggatcaggcg
    22201 cggtcgccac catttccatg tcgctatggc tgttgatgat ttctgtcatg atctggcgca
    22261 tcagtgccga atcatcgaca gataacaccc tgattttgct catcgttaat ccttacttag
    22321 cgcatacacc gtctgaccac gcagcgtgaa gcggcgctca aggtggctaa agttttcaga
    22381 gtgacccgca aacagcaatc cgtcgggttt aaggagcgga acaaagcggc gcaaaatctc
    22441 ctgctgggta ttttgatcga agtagatcat gacgttacga cagaagatcg catcaaacgg
    22501 ccccggcacg gtgtactgtt tctccagtag attcagcggg gcaaaatcaa catagttcgc
    22561 caactcctga cgcacgcgta ccagcccttc atgcggtccc gtccctcgca tgaaataccg
    22621 ttgcagttgc tgcggcgtca ggtttttcaa ctcttcatgg cgataaatac cgcttctggc
    22681 tttttccagc acttcggtgt cgatatcact ggcaaacact ttccagcgtc cgggcgcggt
    22741 gcccaatgtg tcagccagcg tcatcgcaat gctgtacggc tcttcgccgg tcgaagccgc
    22801 cgcgctccat acgcgatact cgccagaacg gcgacgtgcg tgatccgcga gcagagggaa
    22861 atggtgtgcc tcacggaaaa atgccgtcag attcgtggtc agcgaattga taaacgcctg
    22921 ccactcaccg ctgtgctgat tagattccag caagttcaga taatgaccga aatccgttag
    22981 tcccagcgaa cgcaaacgac gaaccagtcg gttgtaaacc atgtcgcgtt tatggtcagc
    23041 cagaacgatc ccggctcgtt gatagatcaa ttgacttatc cgccgaaaat gcgcgtcgga
    23101 aagcgccagg cgctcggtca tctgtaacaa taaagacgtt tgcccacagg gcagagatga
    23161 agtcatagcg ccttctcaat cacttcagga taccactggc gcaatttgta actgcgccga
    23221 ctcatgtcgt gctacttcat gttcttcaag ggtaaatacc gccacacgcg acgaaagatg
    23281 gtcggcctga ttcgccagtt gttccgttgc caccgccgct tcttctacca gcgaggcgtt
    23341 ctgctgagtc acctgatcca tctggctgac agcctgtgca acctgctcaa tcccccgctg
    23401 ttgttcttcc gacgcagagg caatttctcc cataatgtcg ttcacgcggg tcaccgaact
    23461 gacaatatcg atcatggtcg cggcggcgtt attcaccagt ttcgaaccct gctggacacg
    23521 attgactgac tcttcgatca gccctttgat ctcttttgct gcctgcgcgc tacggctggc
    23581 aagattgcgc acttcacctg ccactaccgc aaaaccacgc ccctgctctc cggcgcgagc
    23641 cgcttccact gccgcattca gggccagaat attggtctgg aaagcaattc cgtcgataac
    23701 gctgataatg tcgccaattt tttgcgagct ggtggcgatc tcctgcatgg tgtgagtcat
    23761 ggtactgacc tgaacaccgc ccgcctgcgc cgttgtcgcg gcattttttg ccagttccga
    23821 cgcctgtcgt gcgttatcgg cgttttgccc taccgtggcg gttaattgct ccatactggc
    23881 ggccgtttgt gccagcgatg ccgcctgctg ttcggtacga cttgagagat cgttattgcc
    23941 tgcgacaatc tccgcgatcc caatgtgcat ctcatggctt cccttacgca catcactcac
    24001 cgtcccacgc aaagcctgct gcatggtctt caggctggca aaaatggcgg tgatctcatt
    24061 acgaccatat accgcaatcg gacgcgccag attacccgca gcaatgctgt caaaatggct
    24121 accaataatg gccagtggtt gaacaatcat cttgcgcgtc caccacagcg cactgctgat
    24181 gtagattgcc gcaacaataa tcatgctgat aaacaccagc gccgaaatct gatagttacg
    24241 ctgactttgc gcactggcgg cttccagcac atggttgatc tccagctgcc aggcctcaaa
    24301 gttaacgtca aacgcattct gtgatccctg caccggcgca gtgaggaaat ccgaaagttg
    24361 attactttca agccaggtcg cctgatgttc gagatcgttg tgccagcggg caaaactttt
    24421 ctccgtctct ttttgcaatg ccctgacgtg ctcgttgccc gcagtcatcg ccataaaact
    24481 tttaaacagc gtggtggatt gcgtcagact ggcgcgcgcc gtcgtcatca acgttttaat
    24541 gtcatccgcc ggatagctaa gtgccgtcag agtgcccgct ttgttcagcg cggtactggc
    24601 ctgtaacatt accgcccgag tttgcgctaa tgccgcacgt tgctgattgc tctgctcgac
    24661 ctgattcagt cgttgcaaat cgtcgcgaaa tgcccaaaaa gacatgccgt tactgccaat
    24721 ctgcaagatc ccgcagagaa tcaaaattaa aaacagcgtg gtcgaaattc gaatacgatt
    24781 aaacatcaac gctccccatc aggcggcaat gaccgcgtta gtaaatactc gtcaaaatgt
    24841 ttcccagttt ggatcttgtt cagcaattcg cagtcgtggc tgtgtcggtg gttgctcact
    24901 ggcaggacgg gatggtgttt gcggtttatt ggtgagtggg ctggctgcca gacggaacgt
    24961 ggaaaccgct tgcgttaaac gactcgcctg ttcttccagc gcagcggcgg cggcagctga
    25021 ttcctgcacc agcgatgcgt tctgttgcgt gacgcgatcc atttccgaaa ccgccaatgc
    25081 gacttgatcg atgccacggc tctgttcatc cgatgccgat gcaatctcgc ccataatgtc
    25141 agtcacgcga gtgacagcat tgacgatatt gttcattgtt tccccggcgc tttcgaccag
    25201 caccgaaccg gtatcaacgc gtgagacgga gtcttcaatg agggctttga tctcttttgc
    25261 cgcctgggcg ctacgactgg caagattacg cacttcaccc gccaccacgg caaaaccacg
    25321 gccctgttca cccgcacgcg cggcttcaac cgcggcattc agcgcgagga tattagtctg
    25381 gaaggcaata ccgtcgataa cgctgataat gtcggcaatt ttcttcgaac tatcggcgat
    25441 ctcatgcatc gttttcacta cgccatccac cactttgccg ccgtgctggg cggtgtcgga
    25501 ggcactttgc gccagttgcg aggcctggcg ggcgttatcg gcgttttgct tcactgtcgc
    25561 ggtgagctgc tccatgctgg cggcagtttc ttccagcgcg gatgcctgct gttcagtacg
    25621 ggaggaaaga tcggtgttgc ccgccgcaat ttcacgggta ccggcataga tggcatctga
    25681 accttcgcgg acatgagtga cggtgtcagt caaagagcgt tgcatatgtg aaacgctctg
    25741 cgccaggtcg cccatttcac tgcgcccgtc aatggtcagg gtattcgcca ggttaccacc
    25801 ggcgatttcg cgaatgtgag caataatttt tgccagcgga gtaagcaaca tacggcgaat
    25861 gccgtaccac gccaccagca gaatcaatac caccaccagc gcgataaccg ccagttgcca
    25921 ctgggcaaat cggtaatcat ctgcgttgtc agtgacgata tcgcgataca gtttttcact
    25981 gctgagggcg tactgagcaa acgcttcgcc cattgcattt tgcattccct gggttggctg
    26041 agcgaaataa gctccagtat tgccataatc aagataatca atcagttcag ttaacgctgt
    26101 gtgatagttt ttatattttt catcaatatt acgactggta gcgaccattt caggtaacgg
    26161 tgccatgctt ttgaattttt tataatgagt cgcggcctgg gccaatgttt tcctggcgct
    26221 atcgagcaat tcaactttgg cgttactttg ttgattggag gaatccatca tcatccgcac
    26281 cgctgaacga ctcaggttaa tgcgcgtttg cagcattaaa tcccaggttg acgtcagctc
    26341 gccctgctgt tcccgtaatt gattggaaac cacaaagctc ttctggctat ggtgaaggga
    26401 agaaaaaaac agactgccgg aaataagctg taacagtgcg aataccccca gcaccattac
    26461 caacagcgtg actacgcgga tacggttaat cataaggcac cttcctgaaa acaagttaat
    26521 ctcgttatcg gcaagaaggg gggaaacttt attgctgatg ccacccgccg cgaaattgaa
    26581 ataaaaaacc cgatgcgcag atcatcgggg tcatttcaat tgaggaaatc gggagaatta
    26641 cgccacttct gacgccgcgc tatctaacag cgccatctct tcgctgttca gcagtttttc
    26701 gatgttcacc agaatcaaca tccggtcgcc cagtgcgccc agtccagtga gatattctgt
    26761 tgaaagcgtc acggcaaact ctggtgccgg acgaatttgc tccgccgtca atgaaagcac
    26821 gtctgaaacg ccgtcaacca cgatgccgac cacccgctgt ccgagattca ggacgataac
    26881 taccgtgttg tcgttatagt ccacatccac ctggctgaac ttaattcgta agtcaacaat
    26941 cggcacaata acgccgcgca gattcgtgac gcctttgata aacgctggcg tgttcgcaat
    27001 ccgtgttacc tgatcgtagc cacggatctc ctgcactttc aggatatcaa taccgtactc
    27061 ttcatcacca agggtaaata ccagaaattc ctggcctgac ggctcgctgg ccagctttgt
    27121 tacattcgtc ataccggtca tattgttacc tttttactca ttcaggcggc ggtgttcgcc
    27181 atacgttgtt cgcggtttat cgcctgcaag gcggagacat caacaatcag tgccacgctg
    27241 ccgtcgccaa gaatggtcgc agcagaaatg ccggggactt tgcgatagtt actttcaagg
    27301 tttttaacca caacctggtg ctgaccaatt aattgatcca ccagcaaggc gtagcggcga
    27361 ccgccacttt gtaagatcac cacaattccc tgggtggctt cggttttcgc gcccgcgacg
    27421 ttgaacactt tccacagttc gacgatgggc agatattcac cccgcacttc cagcacccgc
    27481 tcgccgccgg ccagtggatg cagatcggct tcacggggtt gcagtgattc cataacagca
    27541 ttcagcggca gaatgaaaac ttcatccgca acgcgtacgg acatgccgtc gaggatggcc
    27601 agcgtcagcg gcagtaaaat gcggatcgta gtgccagtac cctgcttcga ctggatttcg
    27661 acatgaccgc ccatctcctg gatattacgt ttaacgacgt ccatgccgac gccgcgcccg
    27721 gagacgtcgg tgacctgctc tgccgtggag aagcctggtg caaatatcag catcgcgact
    27781 tcgtcgtcgc tcatgttttc gctgacagtc aaaccttgcg aggccgcttt tgccagaatt
    27841 cgctcacggt ttagccccgc cccatcgtcg gtcacttcaa tgcaaatgtt gccgccctga
    27901 tgttcggcag acagaattaa atttccgacg ctgtttttac ctgcggcgag ccgtttttct
    27961 ggcagttcaa taccgtgatc gaggctattg cgtaccaggt gggtcagcgg gtcgataatg
    28021 cgttctatca ggcttttgtc gagttcagta gaactgccca ccagcgtcag ttctacctgc
    28081 ttgccgagtt ttcccgccag atcacgcacc agccggggat agcgactaaa aacatattcc
    28141 atcggcatca tgcgaatcga catcactgat tcctgcaaat cacgggcgtt acgttgtaac
    28201 tgccccatgc tggttatcaa atcaccatga ttaaccgggt ccagttcgct ggaacgctgg
    28261 gcaagcatgg actgggtgat aaccagctcg ccgacgaggt taattaattg atcaaccttt
    28321 tctaccgcta cacggatgct ggtggattca ttgctgcgcg tcgttttttc ccgctccacg
    28381 cggccggttg gcgcttgttc ggctgccagt ttaagcactg gtggggtgga tatttttggc
    28441 gagacttcta ctgtttcaaa ggtaatctga tcggcttcaa tcacaaaaca gagtaccgct
    28501 gtgatgtcat cttcggcgat gtcgcccggt aatattgccg agagcgaatc cgcccctttc
    28561 accacgtcag ttaacgttgt cagatgtccc agttcttctt ccagcaggtc gacttccccg
    28621 gccttcaggc gcgaaaggat aattcgtcgc ggcgactgac tgcgactctg ctcatcttgc
    28681 ggttcacttt tggcaaccac acttaatcgg gtcactgcgg atggcgtttc gcctttcgct
    28741 tctaatgcca gttgacgcaa ggcctggcag atataatcga agctggcggc atccggctct
    28801 tgcgactgtt tataagcgtc gagctgttct tgcatgatgt ccttcgtttc caaaaacaga
    28861 ttgataatgt cggtgttgag ttgcatctca cctcgtctgg cttcatcgag caggttttcc
    28921 atcagatgcg tggtttcctg caaaacgctg aagccaaaag ttcctgcccc tcctttgatc
    28981 gagtgggcag cccgaaagat ggcattcaat tgttcggcat ctggcgcttc cggctgcaaa
    29041 accagcagat gctgctccat gtcagccaac agttcgtccg cttcatcaaa aaatgtctga
    29101 taaaaatcgc ttatatccat gctcacgctg tcacctcggt tcggctgatg gcattgtggg
    29161 aacactgacc tgtggtgcaa cctcaggttt ttccagggcg cttactggct cattctggct
    29221 ttcggcgttt tcatgcaaaa tggcctgttc ggcttgtttg ttcagtacca gcaggctgat
    29281 gcgacggttg acggcatcat caggtccgcg atcgcttaag cgcatcgttg ccgccatgcc
    29341 gacgacacgt aacactttgc cgctatccaa ccctccgacc atcagttcgc ggcgggatgc
    29401 attggcccga tcggcagaaa gctcccagtt gctatatcct ttctcaccgc tggcgtaggg
    29461 gaaatcatcg gtatgacctg aaaggctaat acggttggga ataccgttca gtacaggcgc
    29521 aatggcacgc agaatgtcgc gcatataggg ttcgacatcg gcactgccgg ttctaaacat
    29581 cgggcgattc tggctatcga tgatctgaat acgtagacct tcctggacca gatcgatttt
    29641 gagatgggga cgtaacgccc gcagtttcgg atcggactct atcaactggt cgagatcacc
    29701 ccgcaatttc cgcaatcgac tttgctccat gcgttttttc agctcttcga tgttcggctg
    29761 cttattcact tccccctggc tttgggtgta atcatcacca ccgccgggaa ttgggctttc
    29821 actattagaa atgcgatcgc cgcccgtaac cgcagtcgcc agtggagtcc ggaagtactc
    29881 cgcaatctga atcagctctt ttgggctgga gatggagatc agccacatca ccagaaaaaa
    29941 ggccatcatc gcagtcataa agtcggcata agcaatcttc cacgatccat gtgctgcccc
    30001 gtggcttttg gctttgcgtc gtttgacgac aataatcgga tgcgcttgat tcttcatgct
    30061 tcctcggttg tcgtctgttg ttgcggattt ttcaccgcac gcacatgctc ttccagttca
    30121 ataaacgacg gacgttcgct ggaatagagc gttttgcgac caaactcaac ggcgataggc
    30181 ggtgcgtaac cgttcagatt agaaagcaga gtgactttga cgcactgcat cattttgctg
    30241 gtttcggcgc ttttctgacg taaaacactc gctaatgggg aaataaatcc gtaagccaat
    30301 aaaatgccga ggaaagtccc caccatcgca tgtgcgataa gcgcacccag ctcggcggca
    30361 ggacgatcgg ctgaacctaa cgcgtgaacg acccccatta cagccgcgac aataccaaac
    30421 gccggaagtg agtccccgac cagcgccaga ctgttcgccg ggacttctgc ctcgctttcg
    30481 tgcgtctcaa tctcttcatc catcagggct tcgatttcga aggtgttcat atgaccgctg
    30541 ataatcagac gcagataatc gacgataaaa tcaagcatga cgctatccgc gaggatgcgt
    30601 gggtagctgg cgaagatctc gctctcacgg ggattttcaa tatcacgttc cagcgaaaac
    30661 atccccatct gccgcgattt cgccatcaac cgataaagca gagccagcag atccatatac
    30721 attgctttgg tgtatttgga gcgacgaaac agcaacggca gcgccttcag cgtgccttta
    30781 atcgctttgc cattattgcc gacgataaac gacccaatcc ctgcaccggc aataatcacc
    30841 agttcagcgg gttgatagag tgctccaagg cttccaccgg tcatcaaata accgccgaaa
    30901 actgtaccga gaacaaccag gtaacctaat aagataagca cgacatcatc cttccactgt
    30961 tgaccatgac aggatgttca gtcgtcaggc gttaacgcgc gattggggca aaaaaaagca
    31021 gcggtacgtc gttaccgctg ctggaatgtt gcgcctcacc gtatcagtta aacagcctgt
    31081 actctctgtt catccagcag ttgtgggata atatcggcag gattctggga aagtttacgt
    31141 ctttttactg cccgggatgg cggttgacat aagctgcagg caaagctgcc aacaggctgg
    31201 tgagcgtggg taataaaatt gccgccgcag cagttgcagc tggaaagttg cagtaatcca
    31261 ctttcaacaa accgcaccaa tgtccaggca cgggttaatg ccagcagtgg tccttcttct
    31321 gcttgtgggc actgttcaag gtataaacgg taggctttga tcaccgcatc gacgccatta
    31381 cacaaaccgg ttttcagtaa aaactgccat gcattacaga acatcgaagc atgaacgttt
    31441 tgttcccagg tcataaacca gtcggttgag aatggcagca tgcctttcgg cggtgggctt
    31501 ccgcgcagtt ctttataaag ttttatcagg cgtccgcgac ttaactgtgt ttcgctttcc
    31561 agcatctgca aacgagcgcc cagggtgatc aattccattg ccagctgaat atcccgcgct
    31621 tcctgaacaa tgcttttttc actcatgatc aggccctttt cttgcgcagc gcttcttcag
    31681 gctgattaac atcattcagc aagcgtgttg agagcatgat gccggtatga atttgctgga
    31741 gatcgtcaac gcgggaatct tgcgtcaact gagtaatcgt ctggtggctg tcaaaacgga
    31801 agtgacaaac cagttgattg gtttctgcca gcttaaccat ttgcggaaga gtcagtgccg
    31861 ctaacgttgt cgccatttct tcatttatgc cgagacgaaa catagcggac gctttgtcct
    31921 gaacaatcaa acgctgtgca agtagtaaat atgacaagtt gatgtcataa atgtgtttca
    31981 gcaactcgga ggtatgcatt attcccaccc agaataacca actttatttt tatgcggttt
    32041 caccgcaccc cgtgatgtcg ccgggaagcc ccggtaaaaa ataattagca ttagaatagt
    32101 tgcgataagc tgcaataagc agaaccacct ttttggttta atatgtcctt acaaatagaa
    32161 atgggtcttt acacttatct aagatttttc ctaaatcgac gcaactgtac tcgtcactac
    32221 acgcacatac aacggagggg ggctgcgatt ttcaataatg tgtgatgcag atcacacaaa
    32281 acactcaatt acttaacata aatgtataag tcatactttt gttttgggtg tatttcaatc
    32341 tgttaaaaag tttttcgcta cgctagcaag caaaaatgaa acaggaataa tcgaaatggg
    32401 atgttgcgca cacttaaaat aactcaccgt aaataatcat ctgctataaa taatcacttt
    32461 catgcaatac cagataagct atttttaaac agacacttac cgcacaacaa actaattaaa
    32521 tacaaagaaa ggcaacaatc aactta
//
LOCUS       DAZFXT010000047        32504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.047, whole genome shotgun sequence.
ACCESSION   DAZFXT010000047 DAZFXT010000000
VERSION     DAZFXT010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.047"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            17..1306
                     /gene="hpaG"
                     /locus_tag="ACHKW0_002325"
     CDS             17..1306
                     /gene="hpaG"
                     /locus_tag="ACHKW0_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008704 -
                     5-carboxymethyl-2-hydroxymuconate delta-isomerase activity
                     [Evidence IEA];
                     GO_function: GO:0018800 -
                     5-oxopent-3-ene-1,2,5-tricarboxylate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HFO7475152.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARSVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1303..2769
                     /gene="hpaE"
                     /locus_tag="ACHKW0_002326"
     CDS             1303..2769
                     /gene="hpaE"
                     /locus_tag="ACHKW0_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7475153.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2771..3622
                     /gene="hpaD"
                     /locus_tag="ACHKW0_002327"
     CDS             2771..3622
                     /gene="hpaD"
                     /locus_tag="ACHKW0_002327"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HFO7475154.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3632..4012
                     /locus_tag="ACHKW0_002328"
     CDS             3632..4012
                     /locus_tag="ACHKW0_002328"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HFO7475155.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4123..4926
                     /gene="hpaH"
                     /locus_tag="ACHKW0_002329"
     CDS             4123..4926
                     /gene="hpaH"
                     /locus_tag="ACHKW0_002329"
                     /EC_number="4.2.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA];
                     GO_process: GO:0009056 - catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hept-4-ene-1,7-dioate hydratase"
                     /protein_id="HFO7475156.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4937..5725
                     /gene="hpaI"
                     /locus_tag="ACHKW0_002330"
     CDS             4937..5725
                     /gene="hpaI"
                     /locus_tag="ACHKW0_002330"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018802 -
                     2,4-dihydroxyhept-2-ene-1,7-dioate aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HFO7475157.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            5900..7276
                     /gene="hpaX"
                     /locus_tag="ACHKW0_002331"
     CDS             5900..7276
                     /gene="hpaX"
                     /locus_tag="ACHKW0_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901241 - 4-hydroxyphenylacetate
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1900754 - 4-hydroxyphenylacetate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HFO7475158.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7286..8176
                     /gene="hpaA"
                     /locus_tag="ACHKW0_002332"
     CDS             7286..8176
                     /gene="hpaA"
                     /locus_tag="ACHKW0_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HFO7475159.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8427..9989
                     /gene="hpaB"
                     /locus_tag="ACHKW0_002333"
     CDS             8427..9989
                     /gene="hpaB"
                     /locus_tag="ACHKW0_002333"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016712 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, reduced flavin or flavoprotein as one
                     donor, and incorporation of one atom of oxygen [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HFO7475160.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10007..10519
                     /locus_tag="ACHKW0_002334"
     CDS             10007..10519
                     /locus_tag="ACHKW0_002334"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HFO7475161.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10912..13062
                     /gene="btsT"
                     /locus_tag="ACHKW0_002335"
     CDS             10912..13062
                     /gene="btsT"
                     /locus_tag="ACHKW0_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HFO7475162.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13112..13315
                     /gene="yjiX"
                     /locus_tag="ACHKW0_002336"
     CDS             13112..13315
                     /gene="yjiX"
                     /locus_tag="ACHKW0_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HFO7475163.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13326..14282
                     /gene="yjiA"
                     /locus_tag="ACHKW0_002337"
     CDS             13326..14282
                     /gene="yjiA"
                     /locus_tag="ACHKW0_002337"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HFO7475164.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(14360..16900)
                     /locus_tag="ACHKW0_002338"
     CDS             complement(14360..16900)
                     /locus_tag="ACHKW0_002338"
                     /EC_number="3.5.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008953 - penicillin amidase activity
                     [Evidence IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin G acylase"
                     /protein_id="HFO7475165.1"
                     /translation="MKNRNRMIVNCVTASLMYYWSLPALAEQSSSEIKIVRDEYGMPH
                     IYANDTWHLFYGYGYVVAQDRLFQMEMARRSTQGTVAEVLGKDFVKFDKDIRRNYWPD
                     AIRAQIAALSPEDMSILQGYADGMNAWIDKVNTNPETLLPKQFNTFGFTPKRWEPFDV
                     AMIFVGTMANRFSDSTSEIDNLALLTALKDKYGVSQGMAVFNQLKWLVNPSAPTTIAV
                     QESNYPLKFNQQNSQTAALLPRYDLPAPMLDRPAKGADGALLALTAGKNRETIAAQFA
                     QGGANGLAGYPTTSNMWVIGKSKAQDAKAIMVNGPQFGWYAPAYTYGIGLHGAGYDVT
                     GNTPFAYPGLVFGHNGVISWGSTAGFGDDVDIFAERLSAEKPGYYLHNGKWVKMLSRE
                     ETITVKNGQAETFTVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAK
                     NWQEWTQQAAKQALTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKG
                     LLPFEMNPKVYNPQSGYIANWNNSPQKDYPASDLFAFLWGGADRVTEIDRLLEQKPRL
                     TADQAWDVIRQTSRQDLNLRLFLPTLQAATSGLTQSDPRRQLVETLTRWDGINLLNDD
                     GKTWQQPGSAILNVWLTSMLKRTVVAAVPMPFDKWYSASGYETTQDGPTGSLNISVGA
                     KILYEAVQGDKSPIPQAVDLFAGKPQQEVVLAALEDTWETLSKRYGNNVSNWKTPAMA
                     LTFRANNFFGVPQAAAEETRHQAEYQNRGTENDMIVFSPTTSDRPVLAWDVVAPGQSG
                     FIAPDGTVDKHYEDQLKMYENFGRKSLWLTKQDVEAHKESQEVLHVQR"
     gene            complement(17252..17416)
                     /gene="yjiS"
                     /locus_tag="ACHKW0_002339"
     CDS             complement(17252..17416)
                     /gene="yjiS"
                     /locus_tag="ACHKW0_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008786105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HFO7475166.1"
                     /translation="MEFHENKAKTPFIRLVQLWHAVRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            17593..19005
                     /gene="yjiR"
                     /locus_tag="ACHKW0_002340"
     CDS             17593..19005
                     /gene="yjiR"
                     /locus_tag="ACHKW0_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HFO7475167.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMRLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DNSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSLALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLM
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWIELPEQVDMVCVARQLCRMNIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYRAME"
     gene            complement(19247..20191)
                     /locus_tag="ACHKW0_002341"
     CDS             complement(19247..20191)
                     /locus_tag="ACHKW0_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7475168.1"
                     /translation="MTNFSTSTPHDALFKSFLTHPGTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFAAPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTECANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPQHRERIMTIAERIYNDGWLLGMEKGKEEGEQRLLRLLL
                     QNGADPEWIQKITGLSTEQMQALEQPLPEIKRDPWIEY"
     gene            20658..21890
                     /gene="mdtM"
                     /locus_tag="ACHKW0_002342"
     CDS             20658..21890
                     /gene="mdtM"
                     /locus_tag="ACHKW0_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HFO7475169.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVGDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTESRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            21931..23211
                     /gene="yjiN"
                     /locus_tag="ACHKW0_002343"
     CDS             21931..23211
                     /gene="yjiN"
                     /locus_tag="ACHKW0_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HFO7475170.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIIPRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRAHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            23327..24478
                     /gene="yjiM"
                     /locus_tag="ACHKW0_002344"
     CDS             23327..24478
                     /gene="yjiM"
                     /locus_tag="ACHKW0_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double-cubane-cluster-containing anaerobic
                     reductase"
                     /protein_id="HFO7475171.1"
                     /translation="MSLITDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDASRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLALRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            24488..25255
                     /gene="yjiL"
                     /locus_tag="ACHKW0_002345"
     CDS             24488..25255
                     /gene="yjiL"
                     /locus_tag="ACHKW0_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="YjiL, as found in Escherichia coli, is a homolog of
                     the activator ATPases of enzymes such as lactoyl-CoA
                     dehydratase, (R)-phenyllactate dehydratase, and
                     2-hydroxyisocaproyl-CoA dehydratase. The typical substrate
                     of those enzymes is acyl-CoA with an OH at the
                     beta-position. YjiL is the putative activator for the
                     cognate protein YjiM, a putative 2-hydroxyacyl-CoA
                     dehydratase with unknown specificity, encoded by the
                     adjacent gene; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-hydroxyacyl-CoA dehydratase activator
                     YjiL"
                     /protein_id="HFO7475172.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            25252..25509
                     /gene="ytiA"
                     /locus_tag="ACHKW0_002346"
     CDS             25252..25509
                     /gene="ytiA"
                     /locus_tag="ACHKW0_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HFO7475173.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGQTESIYCQQDGVWRCIAHYGVSPR"
     gene            25574..26434
                     /gene="yjiK"
                     /locus_tag="ACHKW0_002347"
     CDS             25574..26434
                     /gene="yjiK"
                     /locus_tag="ACHKW0_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdiA-regulated domain-containing protein"
                     /protein_id="HFO7475174.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            26502..27680
                     /gene="yjiJ"
                     /locus_tag="ACHKW0_002348"
     CDS             26502..27680
                     /gene="yjiJ"
                     /locus_tag="ACHKW0_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7475175.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPLLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(27693..28247)
                     /gene="kptA"
                     /locus_tag="ACHKW0_002349"
     CDS             complement(27693..28247)
                     /gene="kptA"
                     /locus_tag="ACHKW0_002349"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HFO7475176.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            28497..29180
                     /gene="yjiH"
                     /locus_tag="ACHKW0_002350"
     CDS             28497..29180
                     /gene="yjiH"
                     /locus_tag="ACHKW0_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HFO7475177.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEMTERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            29177..29638
                     /gene="yjiG"
                     /locus_tag="ACHKW0_002351"
     CDS             29177..29638
                     /gene="yjiG"
                     /locus_tag="ACHKW0_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HFO7475178.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLIGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            29651..30823
                     /gene="iadA"
                     /locus_tag="ACHKW0_002352"
     CDS             29651..30823
                     /gene="iadA"
                     /locus_tag="ACHKW0_002352"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008798 - beta-aspartyl-peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7475179.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            30888..31799
                     /gene="hypT"
                     /locus_tag="ACHKW0_002353"
                     /gene_synonym="qseD"
     CDS             30888..31799
                     /gene="hypT"
                     /locus_tag="ACHKW0_002353"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HFO7475180.1"
                     /translation="MDDCGAILHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(31792..32184)
                     /gene="iraD"
                     /locus_tag="ACHKW0_002354"
     CDS             complement(31792..32184)
                     /gene="iraD"
                     /locus_tag="ACHKW0_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HFO7475181.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNPSDKHLQSWYCRQLRSALLFHEPRIAALQVNLKEAYCHTLAISLEIM
                     LYHDDEPLTFDLVWDNGGWRSATLENVS"
     gene            complement(32181..32264)
                     /gene="idlP"
                     /locus_tag="ACHKW0_002355"
     CDS             complement(32181..32264)
                     /gene="idlP"
                     /locus_tag="ACHKW0_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HFO7475182.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
     gene            complement(32380..>32504)
                     /locus_tag="ACHKW0_002356"
     CDS             complement(32380..>32504)
                     /locus_tag="ACHKW0_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475183.1"
                     /translation="NNFPPELSSGGDKYFHLHNYSEYSEYTSGFFLSLMIFIKS"
BASE COUNT         7452 a         8598 c         8540 g         7914 t
ORIGIN      
        1 aatccgagga gtggtaatga aaggcactat cttcgccgta gcattgaacc atcgcagcca
       61 gcttgatgca tggcaggaag cgttccagca atccccctac aaagccccgc ctaaaactgc
      121 ggtctggttt attaaaccgc gcaatacggt gattggttgc ggtgaaccga ttccctttcc
      181 acagggtgaa aaggtactga gcggtgcgac agttgcgctg attgtgggaa aaacggcgac
      241 gaaagtacgt gaagaagatg cggcagagta catcgccgga tatgcgctgg ctaacgacgt
      301 cagcctgccg gaagagagct tttaccgtcc ggcaatcaaa gcaaaatgtc gtgatggatt
      361 ctgccccatt ggcgaaaccg tggctctcag caatgtcgat aatctgacca tctataccga
      421 gatcaacggg cgtcctgccg atcactggaa caccgccgat ttacaacgta acgccgcgca
      481 gttgctgagc gccctgagcg aatttgccac actgaatcca ggcgatgcca ttctgctcgg
      541 cacgccacag gcgcgcgtgg aaatacagcc aggcgatcgc gttcgtgttc tcgcagaagg
      601 tttcccgccg ctggaaaatc cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt
      661 cccaacgcag ccacacccgc acggcacgct gtttgccctc ggcctgaact acgccgacca
      721 cgccagcgaa ctggaattta agccaccgga agaaccgctg gtgttcctga aagcgccaaa
      781 taccctcact ggcgataacc agacctccgt gcgtccaaac aatattgaat acatgcacta
      841 tgaagcggag ctggtggtag ttattggcaa acaggcgcgt agcgtcagcg aagccgatgc
      901 catggattat gtcgcgggct acaccgtgtg taacgactac gccattcgcg actatctgga
      961 aaactactac cgccctaacc tgcgggtaaa aagccgcgac ggactgacgc cgatgctttc
     1021 aaccatcgtg ccgaaagagg cgatcccgga cccgcataat ctgacccttc gcaccttcgt
     1081 caacggcgag ttacgccagc aaggcaccac cgccgatctg atcttcagcg tgcccttcct
     1141 gatcgcctac ttaagcgaat ttatgaccct gaatccgggc gacatgatcg ccaccggcac
     1201 accaaaaggc ttatctgacg tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt
     1261 gggccgcctg gtgaaccgaa ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat
     1321 tggatcaacg gcaaaaatgt tgcaggtaac gactacttcc agaccaccaa tccggcaacg
     1381 ggtgaagtgc tggcggatgt ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg
     1441 gcagcgaaag aggcgttccc gaaatgggcc aatctgccaa tgaaagagcg tgcgcgcctg
     1501 atgcgccgtc tgggcgatct gatcgaccag aacgtgccag agatcgccgc gatggaaacc
     1561 gcggacaccg gcctgccgat ccatcagacc aaaaatgtgt tgatcccacg cgcttcccac
     1621 aactttgaat ttttcgcgga agtctgccag cagatgaacg gcaagactta tccggtcgac
     1681 gacaagatgc tcaactacac gctggtgcag ccggtaggcg tttgtgcact ggtgtcaccg
     1741 tggaacgtgc cgtttatgac cgccacctgg aaggtcgcgc cgtgtctggc gctgggcaat
     1801 accgcggtac tgaaaatgtc ggaactctcc ccgctgaccg ccgaccgcct gggtgagctg
     1861 gcgctggaag ctggtattcc ggcgggcgtg ctgaacgtgg tacagggcta cggcgcaacc
     1921 gcaggcgatg cgctggtccg tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacg
     1981 gcgaccgggc gcaacatcat gaaaaacgcc gggctgaaaa aatactccat ggaactgggc
     2041 ggtaaatcgc cggtgctgat ttttgaagat gctgatattg aacgcgcgct ggacgccgcc
     2101 ctgttcacca tcttctcgat caacggcgaa cgctgcaccg ccggttcgcg catctttatt
     2161 cagcaaagca tctacccgga attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc
     2221 gtgggcgatc cgaacgatcc gaatacccag gttggcgcgc ttatcagcca gcagcactgg
     2281 gaaaaagtct ccggctatat ccgtctcggc attgaagaag gcgcaaccct gctggcgggc
     2341 ggcccggata aaccgtccga cctgcctgcg cacctgaaag gcggcaactt cctgcgccca
     2401 accgtgctgg cagacgttga taaccgtatg cgagtcgccc aggaagagat tttcgggccg
     2461 gtcgcctgcc tgctgccgtt taaagacgaa gccgaaggct tacgtctggc aaacgacgtg
     2521 gagtacggcc tcgcgtcgta catctggaca caggatgtca gcaaagtgtt acgcctggcg
     2581 cgtggcattg aagctggcat ggtgttcgtc aacacccaga acgtgcgtga cctgcgccag
     2641 ccatttggcg gcgtaaaagc ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa
     2701 gtgttcgcgg aaatgaagaa cgtctgcatt tccatgggcg accatccaat tccgaaatgg
     2761 ggagtctgat atgggtaagt tagcgttagc cgccaagatc acgcacgtac cgtcgatgta
     2821 tctctctgaa ctgccaggga aaaaccacgg ttgccgccag ggcgcgatcg acgggcataa
     2881 agagatcagc aagcgttgcc gggaaatggg cgtcgatacc attatcgttt tcgataccca
     2941 ctggctggtc aacagtgctt atcacatcaa ctgtgcagac cattttgaag gcgtctacac
     3001 cagcaacgag ctgccgcatt ttatccgcga tatgacctac aactacgagg gcaacccgga
     3061 gttggggcag cttattgccg atgaagcctt aaagctcggc gtgcgggcaa aagcgcacaa
     3121 cattcccagc ctgaaactgg aatacggcac gctggtgcca atgcgctaca tgaatgaaga
     3181 caagcacttc aaagtggttt ccatttcagc tttctgcact gttcacgatt ttgccgacag
     3241 ccgcaagctg ggcgaggcga ttctgaaagc gatcgaacag tacgacggca ccgtggcggt
     3301 ccttgccagc ggttcgttat cgcaccgctt tattgacgat cagcgagcgg aagaagggat
     3361 gaacagctac acccgcgagt tcgaccgcca gatggacgag cgtgtggtga agctgtggcg
     3421 cgaaggccag ttcaaagagt tctgcaatat gctgccggag tacgccgact actgctacgg
     3481 cgaaggcaat atgcacgaca cggtgatgct gctggggatg ctcggctggg ataaatacga
     3541 cggcaaggtg gagtttatca ccgagctatt cccaagctct ggcaccggtc aggttaacgc
     3601 tgttttcccg cttcccgcgt aaggacgttt tatgccgcac tttatcgttg aatgcagtga
     3661 caacatccgc gaagaagccg acctgccggg gttgttcgcc aaagtgaatc cgacgctggc
     3721 agccacgggt atttttccgc tggcgggtat tcgcagccgc gtgcattggg tcgatacctg
     3781 gcagatggcc gacgggcagc gtgattatgc cttcgtgcat atgacgttga aaatcggcgc
     3841 aggtcgcagc ctggaaagcc gtcagcaggc gggtgaaatg ctgtttgaac tgattaaaac
     3901 gcacttcgcc gccctgatgg agagccgcct gctggcgttg tcgtttgaga ttgaagagct
     3961 gcatccgacg ctgaatttta aacaaaacaa cgtgcacgca ttgtttaagt agcgcgcaga
     4021 ttgcccggtg gcgctgcgct taccgggcct acaaaacccc aaaccgtaca ccgtaggccg
     4081 gataaggcgc agccgcatcc ggcaatgcca caggatatcg ctatgttcga taaacatacc
     4141 cacaccctga tcgcccagcg tctggatcag gcagaaaaac agcgcgaaca gatccgcgcg
     4201 atctcactgg attacccgga gatcaccatc gaagacgctt acgcggtgca gcgcgaatgg
     4261 gttcgcctga aaatcgccga aggtcgcacg ctgaaaggcc acaaaatcgg cctgacctcg
     4321 aaagcgatgc aggcaagctc gcagatcagc gaaccggatt acggcgcgct gctggatgac
     4381 atgttcttcc acgatggcag cgatatcccg accgatcgct ttatcgtgcc gcgcattgaa
     4441 gtggagctgg ctttcgtgct ggcaaaaccg ctgcgtgggc caaactgcac gctgttcgac
     4501 gtctacaacg ccacggacta cgtgatcccg gcgctggagc tgatcgacgc tcgctgccac
     4561 aacatcgatc cggaaaccca gcgcccgcgc aaagtgttcg acaccatttc tgataacgcc
     4621 gccaacgccg gggtgatcct cggtggtcgt cccattaagc ccgatgagtt ggatctgcgt
     4681 tggatctccg ccctgatgta tcgcaatggc gtgattgaag aaaccggcgt cgccgctggt
     4741 gtgctgaatc atccggcaaa cggcgtggcc tggctggcga acaaactcgc accgtatgac
     4801 gtacaactgg aagccgggca aatcattctc ggcggttcgt tcacccgccc ggttccggcg
     4861 cgtaagggcg acaccttcca cgtcgattac ggcaacatgg gctccattag ctgccgcttt
     4921 gtttaaggag agaacgatgg aaaacagttt taaagcggcg ctgaaagcag gccgcccgca
     4981 gatcggatta tggctggggc tgagtagcag ctacagcgca gagttactgg ccggagcagg
     5041 attcgactgg ttattgatcg acggtgagca cgcgccgaat aacgtgcaaa ccgtgctcac
     5101 ccagctacag gcgattgcgc cctaccccag ccagccggtg gtacgtccgt cgtggaacga
     5161 tccggtgcaa atcaaacaac tgctggacgt cggcacacaa accttgctgg tgccgatggt
     5221 acaaaacgct gacgaagccc gtgaagcggt acgcgccacc cgttatcccc ccgccggtat
     5281 tcgcggtgtg ggcagtgcgc tggcccgagc ctcgcgctgg aatcgtattc ctgattacct
     5341 gcaaaaagcc aacgatcaaa tgtgcgtgct ggtgcagatc gaaacgcgtg aggcaatgaa
     5401 gaacttaccg cagattctgg acgtggaagg ggtcgacggc gtgtttatcg gcccggcgga
     5461 tctgagcgcc gatatgggtt atgccggtaa tccacagcac ccggaagtac aggccgcgat
     5521 tgagcaggcg atcgtgcaga tccgcgaagc gggcaaagcg ccggggatcc tgatcgccaa
     5581 tgagcaactg gcaaaacgct atctggaact gggcgcgctg tttgtcgctg tcggcgttga
     5641 caccaccctg ctcgcccgcg ccgctgaagc gctggcagca cggtttggcg cacaggccac
     5701 gaccgttaag cccggcgtgt attaatgccg gatgcgctgc acttatccgg cctacactcg
     5761 caccaaaccg taggccggat aaggcgttta cgccgcatcc ggcaaaaagc cgtaccaaat
     5821 cgcggaccgg ataaggcgtt tgtgcgcatc cgccataaac cttgtcgtac cctacaaaaa
     5881 tcccattaga ggaagaaaaa tgagcgacac ctcacctgcc ataccggaga gtatcgatcc
     5941 ggcgaatcag cataaagcgc tgactgccgg acaacaggcg gttattaaga agctgtttcg
     6001 ccgcctgatc gtctttctgt tcgtgctgtt tatcttctcg ttccttgatc gcatcaacat
     6061 cggctttgcc ggactcacga tgggacgcga cctcggtctg agcgccacca tgtttggcct
     6121 cgctaccacc ctgttctatg ccgcttatgt catcttcggc attcccagca acattatgct
     6181 gagtattgtc ggtgcgcggc gctggatcgc caccatcatg gtgctttggg gcatcgcctc
     6241 tactgccacc atgtttgcca ctggccccac cagcttgtac gtactgcgta tactggttgg
     6301 cattaccgaa gccggctttc tgcccggcat tctgctgtat ttaaccttct ggtttccggc
     6361 ctatttccgc gcccgtgcca acgccttgtt tatggtagca atgccggtaa cgacagcgtt
     6421 gggctcgatc gtttccggct acattttgtc gctggatggc gtcatggcat taaaaggctg
     6481 gcagtggctg tttttgctgg aaggcttccc gtcggtatta ctcggcgtca tggtgtggtt
     6541 ctggcttgat gactcaccgg acaaagctaa gtggctgacg aaagaagaca aaaaatgcct
     6601 gcaagagatg atggataacg atcgtctgac gctggttcag ccagagggag ccatcagcca
     6661 ccacgccatg caacaacgca gtatgtggcg ggagatcttc actccggtgg tgatgatgta
     6721 taccctggcg tatttctgcc tgaccaacac acttagtgcg atcagcatct ggacaccgca
     6781 gatcctgcaa agttttaatc agggcagcag taatatcacc atcggcctgc tggccgccgt
     6841 accgcagatt tgtaccattc tcgggatggt ctactggagc cgtcactcag atcgccgcca
     6901 ggaacgaagg catcacaccg cccttcctta tttgttcgct gccgcaggtt ggttactggc
     6961 ttcggcaact gatcacaaca tgatccagat gctggggatc attatggctt cgaccggatc
     7021 attcagcgca atggcgattt tctggacaac accggatcag tccatcagcc tgcgggcacg
     7081 agcgatcggt attgcggtga tcaacgccac tggcaacatt ggttcagcgt taagtccgtt
     7141 tatgatcggc tggttgaaag atttgaccgg cagctttaac agtggattgt ggtttgttgc
     7201 cgcgctgctg gtgattggtg cggggattat ctgggcgatt ccaatgcagt cctcccgtcc
     7261 gcgagcgacc ccgtaaggaa cgacgatgtg tgaccgtcag attgccaata ttgatatcag
     7321 caaagagtac gatgaaagcc tgggcacgga cgatgtgcat tatcagtcct tcgcccgcat
     7381 ggcggccttt tttggccgcc atatgctgcc acatcgccac gaacagtact ttcagatgca
     7441 tttcctcaat agcggacaga ttgagctaca acttgacgat catcgctact cggtggaagc
     7501 gcccctgttt gtcctgacgc cgccgtcagt acctcatgcg tttattacgg agtctgacgc
     7561 cgacggtcat gtgttgacgg tacgggaaga tctgatctgg cccctgctgg aagttcttta
     7621 tccgggcact agggaaacct tcggcctgcc ggggatttgc ctgtcactgg cagataaacc
     7681 cgacgaactg gcggcgctgg aacactattg gcaactgata gagcgggaat cggtggaaca
     7741 actgcctgga cgggaacaca ccctgacgtt actggcacag gcagtgttca ccctactgct
     7801 gcgtaacgca aaactcgacg accatgccgc cagcgggatg cgcggagaat taaaactgtt
     7861 ccagcgtttt aatatgctga ttgaaagcca ttttcatcag cactggacag taccggatta
     7921 cgctaacgaa ctgcatatca ccgaatcacg actcacggac atctgccgtc gctttgccaa
     7981 ccgtccgcca aaacggttga ttttcgacag gcagctgcga gaagccaagc ggctgctgct
     8041 gttttctgat aacgccgtga acaatattgc ctggcaactc ggttttaagg atccggctta
     8101 ttttgcgcgc ttttttaatc gcttagtcgg ttgctcgccc agtgcttatc gcgccaaaaa
     8161 agtacctgtg acgtgaaaat cccttagcct taacgggaaa ccaggcacca cctgctattc
     8221 ccctttctcc gcctggagca aggggattta gtaacgtcaa aaatcgctaa aagcgacctc
     8281 gatcacaaaa ccgtacccga cctgaaaagt accagccatt gtcccaaaag tctcttcccg
     8341 taatcgcccc tgcaagcttc aattgaataa caaaaacaac acacaaaatt aacaatcgta
     8401 ttccgattaa tactgtagag gtcgacatga aaccagaaga tttccgcgcc agtacccaac
     8461 gtccgttcac cggggaagag tatctgaaaa gcctgcagga tggtcgcgag atctatatct
     8521 atggcgagcg agtgaaagac gtcactactc atccggcatt tcgtaatgcg gcagcgtctg
     8581 ttgcccaact gtacgacgcg ctacacaaac cggagatgca ggactctctg tgctggaaca
     8641 ccgacaccgg cagcggcggc tatacccata aattcttccg cgtggcgaaa agtgccgacg
     8701 acctgcgcca gcaacgcgat gccatcgctg agtggtcacg cctgagctat ggctggatgg
     8761 gccgtacccc agactacaaa gctgctttcg gttgcgcact gggcgcgaat ccgggctttt
     8821 acggtcagtt cgagcagaac gcccgtaact ggtacacccg tattcaggaa actggcctct
     8881 actttaacca cgcgattgtt aacccaccga tcgatcgtca tttgccgacc gataaagtga
     8941 aagacgttta catcaagctg gaaaaagaga ctgacgccgg gattatcgtt agcggtgcga
     9001 aagtggttgc caccaactcg gcgctgactc actacaacat gattggcttc ggctcggcac
     9061 aagtaatggg cgaaaacccg gacttcgcac tgatgttcgt tgcgccaatg gatgccaatg
     9121 gcgtcaaatt aatctcccgc gcctcttatg agatggtcgc gggtgctacc ggctcgccgt
     9181 acgactaccc gctctccagc cgcttcgatg agaacgatgc gattctggtg atggataacg
     9241 tgctgatccc atgggaaaac gtactgatct accgcgattt cgatcgctgc cgtcgctgga
     9301 cgatggaagg cggttttgcc cgtatgtatc cgctgcaagc ctgtgtgcgc ctggcagtga
     9361 aactagactt cattacggca ctgctgaaaa aatcactcga atgtaccggc accctggagt
     9421 tccgtggtgt gcaggccgat ctcggtgaag tggtggcgtg gcgcaacacc ttctgggcat
     9481 tgagtgactc gatgtgttct gaagcgacgc cgtgggtcaa cggggcttat ttaccggatc
     9541 atgccgcact gcaaacctat cgcgtactgg caccaatggc ctacgcgaag atcaaaaaca
     9601 ttatcgaacg caacgttacc agtggcctga tctatctccc ttccagtgcc cgtgacctga
     9661 acaatccgca gatcgaccag tatctggcga agtatgtgcg cggttcgaat ggtatggatc
     9721 acgtccagcg catcaagatc ctcaaactga tgtgggacgc cattggcagc gagtttggtg
     9781 gtcgccacga actgtatgaa atcaactact ccggtagcca ggatgagatt cgcctgcagt
     9841 gtctgcgcca ggcacaaagc tccggcaata tggacaagat gatggcgatg gttgatcgct
     9901 gcctgtcgga atacgaccag aacggctgga ctgtgccgca cctgcacaac aacgacgata
     9961 tcaacatgct ggataagctg ctgaaataac gcagcaggag gttaagatgc aattagatga
    10021 acagcgcctg cgctttcgtg acgcgatggc cagcctgtcg gcagcggtaa atattatcac
    10081 caccgagggc gacgccggac aatgcgggat tacggcaacg gccgtctgct cggtcacgga
    10141 tacaccacca tcgctgatgg tgtgcattaa cgccaacagt gcgatgaacc cggtttttca
    10201 gggcaacggc aagttgtgcg tcaacgtcct caaccatgag caggaactga tggcacgcca
    10261 cttcgcgggc atgacgggca tggcgatgga agagcgtttt agcctctcat gctggcaaaa
    10321 aggtccgctg gcgcagccgg tgctaaaagg ttcgctggcc agtcttgaag gtgagatccg
    10381 cgatgtgcag gcaattggca cacatctggt gtatctggtg gagattaaaa acatcatcct
    10441 cagtgcagaa ggtcacggac ttatctactt taaacgccgt ttccatccgg tgatgctgga
    10501 aatggaagct gcgatttaag ctccagcccc ttcacgcatt cgtcgaaggg gcatttttat
    10561 tgtgatctca atcacatatc cacccgatga ataattcttg cccaaccgct caccgccatt
    10621 cctgaccgct tacatcccta aaataaccac tcagttattt accttacttt acgcgcgcgt
    10681 aactctggca acatcactgc aggatagcgg tcaatttacc tcctcaaacg caacgcaaac
    10741 ctagaacggc ttcggcctat gattaacaaa ttactctatg aattttggtt tgcaggaagg
    10801 cagcaagtga gtgaatcccc gggagcttac aacagtaagt gacaggggtg aacgaacgca
    10861 actgccgcac ctgtaagcca aaagacgacg agtaaaatgc caggttttac tatggatacg
    10921 aaaaaactat tcaagcacat accctgggtg attctcggaa tcatcggtgc attctgcctc
    10981 gcggtagttg cattacgtcg gggggagcac gtcagcgccc tgtggatcgt ggtcgcctct
    11041 gtgtcggtgt atctggtggc gtatcgctac tacagtctgt acatcgccca gaaggtgatg
    11101 aaactcgacc ccacgcgcgc gacgcctgcg gttattaaca acgacggtct gaactacgtt
    11161 ccgaccaacc gttacgtgtt gtttggtcac cacttcgccg ctatcgccgg tgctggtccg
    11221 ctggtcggtc cggttctcgc cgcacagatg ggctacctgc ctggcacgct gtggctgctg
    11281 gcgggggtag tactggccgg tgcggttcag gactttatgg tgctgtttat ctcctctcgc
    11341 cgtaacggcg catctctcgg tgagatgatc aaagaagaga tgggaccagt accggggact
    11401 atcgcgctgt ttggctgttt cttaatcatg atcatcatcc tcgccgtcct ggcgctgatt
    11461 gtggttaaag ccctggccga aagtccgtgg ggtgtcttca ccgtttgctc aaccgtaccg
    11521 attgcgctgt ttatgggtat ctacatgcgc tttatccgtc cggggcgtgt gggtgaagtc
    11581 tctgtcattg gtatcgtgct gctggttgcc tctatctact tcggtggcgt gattgctcac
    11641 gatccgtact ggggtccggc actgaccttt aaagacacca ccattacctt cgcgctgatt
    11701 ggctatgcgt ttgtttccgc actgctgccg gtgtggctga tcctcgcacc gcgcgactat
    11761 ctggcaacct tcctgaaaat cggcgttatc gtcggcctgg cgctgggtat cgtggtgctg
    11821 aacccggaac tgaaaatgcc tgcgatgacc cagtacattg acggtactgg cccgctgtgg
    11881 aaaggcgctc tgttcccgtt cctgttcatc accatcgcct gtggtgcggt atctggcttc
    11941 cacgcgctga tctcttccgg tacgacgcca aaactgctgg ctaacgaaac cgacgcgcgt
    12001 ttcattggct acggcgcaat gctgatggag tccttcgtgg cgattatggc gctggttgca
    12061 gcgtccatca tcgaaccggg tctttacttc gcgatgaata ccccgcctgc tggccttggc
    12121 atcaccatgc ctaacctgca tgaaatgggt ggcgagaacg cgccgatcat catggcgcag
    12181 ctgaaagacg ttaccgcaca cgcggcagcg accgtcagct cctggggctt cgtgatttcg
    12241 ccagagcaga tcctgcaaac cgcgaaagac attggtgagc cttctgtcct gaaccgtgca
    12301 ggtggtgcgc caacgctggc ggtaggtatc gctcacgtgt tccacaaagt gctgccgatg
    12361 gctgacatgg gcttctggta tcacttcggt attctgttcg aagccctgtt catcctgacc
    12421 gcgctggatg cgggtactcg ttctggccgc tttatgctgc aagacctgct gggtaacttc
    12481 atcccgttcc tgaaaaaaac cgactctctg gttgccggta tcatcggtac tgcgggctgt
    12541 gtgggtctgt ggggctacct gctgtatcag ggcgtggtcg atccgctggg cggcgttaag
    12601 agcctgtggc cgctgttcgg tatttccaac cagatgctgg cagccgtagc gctggtactg
    12661 ggcaccgttg tgctgattaa gatgaagcgc acccaataca tctgggtaac tgttgttccg
    12721 gctgtatggc tgcttatctg caccacctgg gcgctgggtc tgaaactgtt cagcaccaac
    12781 ccgcagatgg aaggcttctt ctacatggca agccagtaca aagagaagat tgctaacggt
    12841 actgacctga cggcgcagca gattgccaac atgaaccaca tcgttgtgaa caactacacc
    12901 aacgcgggcc tgagtattct gttcctgatt gtggtgtaca gcatcatctt ctacggtttc
    12961 aaaacctggc ttgcggtgcg taacagcgac aaacgtactg acaaagaaac accgtacgtt
    13021 ccaatcccgg aaggcggcgt gaagatctct tcgcaccact aaccgtattt agccccgctt
    13081 cggcggggct ttgttcttat cagagtgaac tatgtttggt aacttaggac aggcaaaaaa
    13141 atatctcggc caggcggcga agatgttgat tggcattcca gactatgaca actacgtcga
    13201 gcatatgaag accaaccatc ccgacaagcc gtacatgagc tatgaagaat tcttccgcga
    13261 acgccagaac gcacgctacg gcggcgatgg taaaggcggt atgcgctgtt gttaatggag
    13321 agagcatgaa cccgattgca gttaccctac tcaccggttt tttaggcgcg ggcaaaacca
    13381 ccctgctgcg ccatattctt aatgaacaac acggctacaa gattgccgtg attgaaaacg
    13441 aattcggcga agtctccgtt gatgatcaat tgattggtga tcgcgcaacg cagatcaaaa
    13501 cgctgaccaa cggctgcatc tgctgttcgc gctccaacga gctggaggac gcgctactcg
    13561 acctgctgga taatctcgac aagggcaata ttcagttcga tcgtctggtc attgaatgca
    13621 ccggcatggc cgatcctggc ccgattattc aaaccttttt ctcccatgag attttatgcc
    13681 agcgctacct gctggacggc gtgattgcgc tggtggatgc ggtacatgcc gatgagcaga
    13741 tgaaccagtt caccatcgcc cagtcccagg tgggctacgc cgaccgtatt ctgctgacta
    13801 aaaccgacgt cgcaggcgaa gcagaaaaac tgcgtgaacg cctggcgcgc atcaacgccc
    13861 gtgcaccggt ctacaccgtc acccacggcg acatcgacct gggcctgctg ttcaacacca
    13921 acggttttat gctggaagaa aacgtcgtca gcaccaaacc gcgtttccac tttatcgcgg
    13981 ataaacaaaa cgatatttcg tcgattgtgg tggaactgga ttacccggta gatatcagcg
    14041 aagtttcccg ggtgatggaa aacctgctgc tggaatcggc ggataaactg ctgcgttaca
    14101 aagggatgct gtggattgac ggcgaaccta accgcctgct gttccagggc gtccagcgcc
    14161 tctacagcgc cgactgggac aggccatggg gcgatgaaac accgcatagc acgatggtgt
    14221 ttatcgggat tcagttgccg gaggatgaga ttcgggcggc gtttgcggga ctcaaaaagt
    14281 aaccagccct ccaacatcac aatacctgag actttatcac cctcatattc ctccagaata
    14341 tgagggcttt cgggcttaat tatctctgaa cgtgcaacac ttcctgcgac tccttatgcg
    14401 cctccacatc ctgcttcgtt aaccagagcg acttacggcc aaaattttcg tacattttca
    14461 gctgatcttc atagtgctta tcaactgttc catcgggagc aataaaccca ctctgaccgg
    14521 gtgcgaccac atcccaggca agcacaggac gatcgcttgt cgttggtgag aaaacaatca
    14581 tatcgttttc tgttccacgg ttttgatact ccgcctgatg acgcgtttct tccgctgcgg
    14641 cctgcggtac accaaagaaa ttatttgccc ggaacgttaa ggccattgca ggtgttttcc
    14701 agttactcac attattgcca tagcgtttgg aaagagtctc ccaggtatct tccagcgcag
    14761 ccaacacaac ctcctgctgt ggtttcccag caaacagatc aaccgcctgt gggattggtg
    14821 atttgtctcc ctgcaccgcc tcatacaaaa tttttgctcc aacacttata ttcagcgaac
    14881 cagttgggcc gtcctgggtt gtttcgtagc cactggcgct gtaccactta tcaaatggca
    14941 taggtacggc agccactacg gtacgcttca acatactggt cagccaaacg ttcaggatgg
    15001 cagagcctgg ctgctgccag gttttaccat catcattaag caaattgatg ccatcccaac
    15061 gtgttaatgt ttctaccaac tgacgacgcg gatcgctctg tgtcaaacca gatgtcgctg
    15121 cttgcagagt aggtaaaaaa agcctcaggt taagatcctg acgactggtt tggcgaataa
    15181 catcccatgc ctgatcagca gttaagcgtg gcttttgctc aagcagtcgg tcgatctccg
    15241 taacgcgatc tgcaccaccc cacaaaaagg caaacagatc tgaagcggga taatcttttt
    15301 ggggagaatt gttccagtta gcaatatatc ccgactgggg gttatacacc ttagggttca
    15361 tttcaaaagg caatagccct ttccagtccc attttcccgt accaggaacg ggtaatcgcg
    15421 gatcatggcc tgattgacga tctggataag caccagtatg aacataacca atattgccgt
    15481 ttacatcagc atagtaccag ttgatggtca gtgcttgttt cgctgcctgc tgtgtccact
    15541 cctgccaatt tttggccttc atctgatgag tccaggccag caaagacgcc acctctttac
    15601 catcccatgc gcgggattta gcgtaagccg tttgtgtcgt ctggtcagtt tggagaatgt
    15661 tgccatgcac cgtacgccag acagtaaagg tctctgcctg accatttttc accgtaatgg
    15721 tttcctcacg gcttaacatt ttcacccact taccattatg caagtagtag cctggtttct
    15781 ctgccgacag ccgttcagca aaaatatcga catcatcgcc gaaacctgcc gttgatcccc
    15841 aggaaatcac accattatga ccaaaaacca gcccaggata ggcaaatggt gtattgccag
    15901 tgacatcata accagcaccg tgcagaccaa taccataagt atacgcaggc gcataccagc
    15961 caaactgcgg accatttacc atgattgctt tcgcatcctg ggctttgctt ttgccgatca
    16021 cccacatatt gctggtcgtt ggataccccg ccagaccatt ggcaccaccc tgtgcaaatt
    16081 gtgcagcaat agtttcccgg ttcttccctg ctgttaacgc cagcagtgcg ccatccgccc
    16141 cttttgctgg tcggtcaagc attggtgcag gtaaatcgta gcgtggcaac agagctgctg
    16201 tttgcgagtt ttgctgatta aatttaagtg ggtagttact ctcttgtacg gcaatagtgg
    16261 ttggcgctga tgggtttacc agccatttca actgattaaa taccgccatg ccttgtgata
    16321 caccatattt atcttttaaa gccgttagca gtgccagatt atcaatttcg ctagtgctat
    16381 cagagaagcg gtttgccatg gtgcccacaa atatcatcgc gacatcaaac ggttcccagc
    16441 gcttaggagt aaagccaaat gtattaaact gttttggtaa gagcgtctct ggattggtat
    16501 ttaccttatc aatccaggca ttcattccat cagcgtagcc ttgcagaatg gacatatcct
    16561 ctggggaaag ggcagcaatt tgcgcccgga tagcatccgg ccagtagtta cgacggatat
    16621 ctttatcaaa tttcacaaaa tctttgccaa gcacttccgc gacagtccct tgagtactgc
    16681 gacgtgccat ttccatctga aaaaggcgat cttgtgctac tacatagcca tagccataaa
    16741 ataggtgcca tgtatcattg gcataaatat gcggcatgcc gtattcatcg cgaacaatct
    16801 ttatctcact tgacgactgc tcagccagtg caggtaagct ccaataatac atcagggaag
    16861 cagtaacaca gttcacgatc atacgatttc tatttttcat tgtatcctct ggcaggtgta
    16921 taattagcga attgatacta gcaacgaagc ttcggttatc tatttgcaga gaattgtttc
    16981 attatgaact gtgagcgcga taactaattt ttgatacaaa aatttcatta atgtgatcta
    17041 aaacgaatcg ctttattcac cgctgatgat tttgttctat tcctttttgt gacagatccc
    17101 ccggacgcat agatgatgac ccccagagag acagctcagg tcattatctg tatgtaaatc
    17161 agaaaaatat gttgtaaacg acttttatgg ttggattttg tagcaatttt gaatgcagac
    17221 aggcagcatt tgccaacacg ccgcccatag ttcactccac atcctccctg cgtaacccaa
    17281 tatccctcaa ccgttcatca ctcatctgct gtaacactcg tcgggtctgc atttgccgcc
    17341 gccaacgcct caccgcatgc cagagttgca caagtctgat aaacggcgtt ttggctttgt
    17401 tttcgtgaaa ttccatctgc tgcctcctca tctttcagag gcttcatcat ccgccttctg
    17461 gcagttcaca atacagatgc aaatattagt tttatttaac atacagacag gttaaaacag
    17521 catctgcatc cggtttttta gcctcatctg taccggtttt tctttctgta tgggccaaat
    17581 aaggaataca gcatgacgcg ttatcaacat ctggcgactc tacttgccga gcggattgag
    17641 caagggctgt atcgtcacgg ggagaaattg ccgtcggtgc gcagcttaag tcaggagcac
    17701 ggcgtcagca tcagcaccgt gcaacaggcg tatcagacgc tggaaacgat gaggctcatc
    17761 actccgcagc cgcgttcggg ttattttgtc gcacaacgta aagcccagcc gcctgtaccg
    17821 ccgatgacgc gtccggtgca gcgcccggtg gaaattaccc agtgggatca ggtgctggat
    17881 atgctggagg cgcatagcga caattccatt gttccgttaa gcaaaagcac gccggatgtc
    17941 gaaacgccca gcctgaaacc gctgtggcgt gagctaagcc gggtggtgca gcataatctg
    18001 caaaccgttc tcggttatga cttgttagcc ggtcagcgag tgttgcggga gcagattgcc
    18061 cgcctgatgc tcgacagcgg ctcggtggtc accgccgatg acatcatcat caccagcggc
    18121 tgccataact cgatgtcgct ggcgttaatg gcggtgtgta aaccgggcga tattgtcgcg
    18181 gtcgaatctc cctgttatta cggttcgatg cagatgctgc gaggcatggg cgtgaaagtg
    18241 attgaaatcc caaccgatcc agaaactggc atcagcgttg aagcactgga actggcgctg
    18301 gaacagtggc cgattaaagg catcattctg gtgccaaact gtaataatcc gctgggattt
    18361 attatgccgg acgcacgcaa acgggccgtt ctctctctcg ctcagcgtca tgatattgtg
    18421 atttttgaag atgatgtcta cggcgaactg gcaacggagt atccgcgccc gcggaccatc
    18481 cattcatggg atatcgacgg gcgagtgctg ttgtgcagct cgttcagtaa aagtattgct
    18541 ccaggcctgc gcgtgggttg ggtcgcaccg gggcgttatc acgataaact gatgcatatg
    18601 aaatacgcca tcagcagctt taatgtgccg tccacgcaaa tggcggcggc aacgtttgtg
    18661 ttggaaggcc actatcatcg ccatatccgg cggatgcggc agacttatca gcgcaatctg
    18721 gcgctttata cctgctggat acgggaatat tttccctgcg aaatctgtat tacgcgcccg
    18781 aaaggcggat ttttactgtg gatagaattg cctgaacagg tcgatatggt ctgcgttgcg
    18841 cggcagctgt gccgcatgaa tatccaggtg gcagcaggct cgattttctc ggcttccggc
    18901 aaataccgta attgtctacg catcaactgc gctttgccgc tcagcgaaac ctatcgcgaa
    18961 gcactaaagc aaattggcga ggccgtgtat cgggcaatgg aataaattcg aattagtggc
    19021 gaagcacctg atttcctctc acgaatcgcc tgtaactcgt tgtatctttg cagggtcatc
    19081 gccacgttcc agcatggctt aggcaaagtg tgcttcgttc ataccggatg cggcgtgaac
    19141 gccttatccg gcctacaaac cgcgcaaatt caatagattg cagaggaaat gtaggtctga
    19201 taagcgtagc gcatcaggca gctttgcgtt tgtcatccgt ctctgattag tattcgatcc
    19261 atggatcgcg cttgatttca ggcaagggct gctctaatgc ctgcatttgc tctgtcgaaa
    19321 gtccggtaat cttttgtatc cattcaggat ctgccccatt ctgcaacaac aatctaagga
    19381 ggcgttgttc cccttcttct ttcccctttt ccatccccaa cagccatcca tcattataaa
    19441 ttcgctctgc aatcgtcatt attcgctccc tgtgttgtgg cattcgacgg gtaagttcgc
    19501 tgataaacgc cttaaaacgc gcttcatcgc cagtcagtaa aatgtaattt aacagcgcag
    19561 ttatctggct gtcattagca cactctgtaa ccagtaatac taccagttgg tcaataagcc
    19621 ccatcagatc gcgctggcga atatgctttt ggatcaactc caacagggcg actctgcgat
    19681 gctgcacaat ctcgtcgtct ggcacgacag tgacatccac cagcgggaac gctgcgctat
    19741 aaagtttccg tgcggtagtc ggggcggcaa attcgtccag ccagcacagg gaccaggggt
    19801 aaggactacg gctaccgtga taaaacagca tcgggataac cagcggtagc ggccggcgtt
    19861 tatcatgctc gatatggcgc tgcatcaccg ccatggaata tcgcatcagg cgaaacgcca
    19921 tatgaatatc ctcgcggctc tgatgttcaa tcactacgta aatataacca tcaccttcac
    19981 gggtctttac cgaccacaga atatcggagt gtagcgcccg caatttttca tcgacaaagc
    20041 tggcggattc cagttttaag ctgtcgagat cgcacagttc acgtaaatct ttaggtaagt
    20101 ggatctccat aaaatcccgc gcggtgccag ggtgcgtgag aaaggactta aataatgcgt
    20161 catgcggcgt gctggtcgag aagtttgtca tggcgatccg tcacctgtag ataagatggc
    20221 ccgactttag cggctaacaa aaccattctc acccggcaat ttcccgttcc ccgaggcgct
    20281 ttccaggcta tttttattct tctgcaaacg atacattttt tcggaaacag gctcgcgttt
    20341 tacatttcct gtacccggca gatattcccc tttccaccgc gctcttttga ttaaccgcaa
    20401 attttaatta cacttaaggt gtatattttc tatgcaaccc atcaattaaa gaggtgtaat
    20461 gtgctgatga ctatttgtaa tcgttataca ttctgacctg gagtcagaag gtatttctct
    20521 gtctgtgtgt tcacaggcag tgtggttgat tacatgaaat tcagtacatt tgtccttctc
    20581 gcctgccttt cgtcattacc gacggtattg aatttcgttt tccccgttgg ggttctccgg
    20641 acaaggagtt gtttgttatg ccacgttttt ttgcccgcca tgccgccacg ctgtttttcc
    20701 cgatggcgtt gattttgtat gactttgccg cgtacctgtc gacggatctg atccagccgg
    20761 ggatcattaa tgtggtcggt gattttaatg ccgatgtcag tctggccccg gcagccgtca
    20821 gtctctatct cgcgggcggt atggcgttac agtggctgct ggggccgctt tccgacagga
    20881 ttggccgcag gccggtgctg attaccgggg cgctaatatt tacccttgcc tgcgctgcga
    20941 caatgttcac aacgtctatg acacagtttc ttatcgcgcg tgcaattcag ggcaccagta
    21001 tctgttttat tgccaccgtt ggttatgtca cggtgcagga ggcgttcggc cagacaaaag
    21061 ggatcaagtt gatggcgatt atcacctcca tcgtactgat tgcgccgatt attggcccgc
    21121 tttccggcgc agctctgatg cactttgtgc actggaaagt cctttttgcc atcattgcag
    21181 ttatgggttt tatctcattt gttggcttac tgctggcgat gccagagacg gtgaagcgcg
    21241 gcgcggttcc atttagcgcc aaaagcgtct ttcgcgattt tcgtaatgtc ttttgcaatc
    21301 ggctgttcct ctttggcgca gcaaccatct ctttaagcta tatcccaatg atgagttggg
    21361 tggctgtctc gccggtgatc ctgatcgacg cgggcggctt aacaacttcg cagttcgcct
    21421 ggacacaggt tccggtgttc ggcgcggtga ttgtcgcgaa tgccatcgtg gcgcgtttcg
    21481 ttaaagatcc gaccgaatcg cgctttatct ggcgtgctgt acccattcaa ctggtcggcc
    21541 tcgcactgtt gattatcggc aatctgctgt caccgcacgt ctggctgtgg tcggtgctgg
    21601 gcaccagttt gtatgctttc gggattggtt tgatcttccc gaccttattc cgctttacgc
    21661 ttttttccaa taacttaccg aaagggaccg tctccgcatc gctgaatatg gtgatcctga
    21721 tggtgatgtc tgtctcggtc gaaatcggcc gctggctgtg gtttaacggc ggtcgcttgc
    21781 cgtttcatct gttagccgtc gtggcgggcg ttatcgtcgt tttcaccctg gcaggattgc
    21841 ttaatcgcgt gcgccaacat caggctgccg agctggcgga ggagcggtga tttttgcgcg
    21901 atcctgccgt caggctctat tcttaacgtt atgaataaac tcattgaact cagacgcgcc
    21961 aaaatgttgg cgctctcttt actgcttatc gccgctgcta cctttgtcgt tacgctgttt
    22021 ttgccgccca atttttgggt gagcggcgtg aaggcgattg ctgaagcggc gatggtcggc
    22081 gcgctggcgg actggtttgc ggtggtggcg ctgtttcgcc gcgtgccgat tccgatcatt
    22141 tctcgtcata cggcgattat cccgcgtaat aaagaccgga ttggcgaaaa tctcggccag
    22201 ttcgtgcagg aaaaatttct cgatacccag tcgctggtgg cattgattcg acgtcacgaa
    22261 ccggcgttgc tgattggcaa ctggtttagc cagccagaaa acgcccgccg cgttggtcag
    22321 catctgttgc agatcatgag tggttttctt gaactgaccg atgatgcgcg tattcagcgc
    22381 ctgcttaagc gcgcggtcca tcgggcgatt gataaggtcg atctttccgg aaccagtgcg
    22441 ttgatgctgg agagtatgac caaaaacgat cgtcatcagg tgctgctcga tacgctgatc
    22501 gcacagttga tcgcccttct ccagcgcgat aaatcgcgca agtttattgc ccagcagatt
    22561 gttcgctggc tggagagtga gcatccactg aaagccaaaa ttctccccac cgaatggttg
    22621 ggcgaacata gcgcggagtt ggtttctgac gcggtgaatt ctttacttga tgatatcagc
    22681 cgtgatcgtg cgcatcagat ccgtcatgcg tttgatcgcg ccacttttgc cctgatcgac
    22741 aagttgaaaa acgatccgga aatggcagcg cgagccgatg ccgtaaaaag ttatctgaaa
    22801 gaagatgaag cttttaaccg ctatctcagt gaattgtggg gggatttacg ggagtggctg
    22861 aaagcggata tcaacagtga agattctcgt gtgaaagaac gtatcgcgcg ggcgggtcaa
    22921 tggtttggcg aaacgttaat tgccgatgat gccttgcggg cgtcgttaaa tggtcacctg
    22981 gaacaagccg cgcaccgcgt cgcgcctgag ttttccgcat tcctgacgcg ccacatcagc
    23041 gatacagtaa aaagctggga tgcgcgggat atgtcgcggc aaatcgagtt aaatatcggc
    23101 aaagatctgc agtttatccg tgtcaacggt acgctggttg gcggttgtat tgggctaatt
    23161 ttatatttgt tgtcgcagct cccggccttg ttccccctcg gcaattttta gaaatccatg
    23221 aacaactaag tggctaaccg cgagagagat caaataaacc acactaaaag aatggtaata
    23281 atgtggcctc ttttcattat ctcccgtggt acggggaagg aaaatcatgt cacttatcac
    23341 cgatctaccc gccattttcg atcagttctc tgaagctcgc cagaaaggct ttctcaccgt
    23401 catggatctc aaggagcgcg gcattccgct ggttggcact tactgcacct ttatgccgca
    23461 agagatcccg atggcagccg gtgcggttgt ggtttcgctc tgttctacct ctgatgaaac
    23521 cattgaagaa gcggagaaag atctgccgcg caacctctgc ccgctgatta aaagcagcta
    23581 cggcttcggc aaaaccgata aatgccccta cttctacttt tcggatctgg tggtcggtga
    23641 aaccacctgc gacggcaaaa agaaaatgta tgaatacatg gcggagttta agcccgttca
    23701 tgtgatgcag ttgccgaaca gcgttaagga cgatgcctcg cgtgcgttat ggaaagccga
    23761 gatgctgcgc ttgcaaaaaa cggtagaaga acgttttggg cacgagatta gcgaagatgc
    23821 tctgcgcgat gccattgcgc tgaaaaaccg cgaacgtcgc gcactggcta atttttatca
    23881 tcttgggcag ttaaatcctc cggcgcttag cggcagcgac attctgaaag tggtctacgg
    23941 cgcaaccttc cggttcgata aagaggcgtt gatcaatgaa ctggacgcga tgacagcccg
    24001 cgttcgtcag cagtgggaag aaggccagcg gctggccctg cgtccacgca ttttaatcac
    24061 cggctgcccg attggcggcg cagcagaaaa agtggtgcgc gcgattgaag agaatggcgg
    24121 ctgggttgtc ggttatgaaa actgcaccgg ggcgaaagcg accgagcaat gcgtggcaga
    24181 aacgggcgat gtctacgacg cactggcgga taaatatctg gcgattggct gctcctgtgt
    24241 ttcgccgaac gatcagcgcc tgcaaatgct cagccagatg gtggaagaat atcaggtcga
    24301 tggcgtagtt gatgtgattt tgcaggcgtg ccatacctac gcggtggaat cgctggcgat
    24361 taaacgtcat gtgcgtcagc agcacaacat tccctatatc gctattgaaa cagactactc
    24421 cacctcagat gtcgggcagc tcagtacccg tgtcgcggcc tttattgaga tgctgtaagg
    24481 agtggcagtg acatattcga ttggcattga ttctggttct accgccacca aagggatctt
    24541 actggcagac ggcgtgatta cgcgccgttt cctcgttcca acaccctttc gtccggcaac
    24601 agcaattact gaagcctggg aaactctgcg cgaagggtta gagacaacgc cgtttctgac
    24661 gctcaccggc tatggacggc aactggtgga ttttgccgat aaacaagtga cggaaatctc
    24721 ctgtcatggg ctgggcgcac ggtttcttgc gccagcaacg cgcgcggtaa tcgacatcgg
    24781 tggtcaggac agcaaagtga ttcagcttga tgatgacggt aacctgtgcg atttcctgat
    24841 gaatgacaaa tgcgcggcgg gcaccgggcg tttcctggag gtgatctcgc gcacgcttgg
    24901 caccagcgtc gagcaactcg acagcattac cgaaaatgtc acgccgcacg ccatcacgag
    24961 tatgtgcaca gtgtttgccg aatcagaagt gatcagcctg cgctcagcgg gcgtcgcgcc
    25021 agaagcgatt ctcgccggag tgattaacgc gatggcgcgg aggagtgcca atttcattgc
    25081 tcgtctctcc tgtgaagcgc cgattctgtt tactggtggc gttagtcatt gccagacgtt
    25141 tgcccggatg ctggaatctc atctgggaat gccggtaaat acccatcctg atgcgcaatt
    25201 tgctggcgca attggcgcgg cggtaattgg tcaacgagtg aggacacgcc gatgaaagag
    25261 tttttatttt tatttcactc cacggtcggc gtcatacaaa cccgcaaggc gttgcaggca
    25321 gcgggcatga cttttcgcgt cagcgatatt ccccgtgatt tacgcggcgg ctgcgggtta
    25381 tgcatttggc tgacctgtcc ccccggcgag gaaatacaat gggtgatccc tgggcagact
    25441 gagtcaattt attgccagca ggatggtgtc tggcgctgca ttgcacatta cggggtttct
    25501 ccgcgttaag gattcattaa ggtcgctacg tatacttctc gatatcttat tgatttgcct
    25561 taaagggatt accgtgacaa aaagtatttc gttaagtaaa cgcatctccg ttattgtgat
    25621 tctttttgcc attgtggcag tgtgtacttt cttcgtgcaa tcttgcgccc gtaaaagcaa
    25681 tcatgcagca tcatttcaga attaccacgc caccattgac ggcaaagaaa ttgcaggcat
    25741 aacgaataat atctcttcgc tgacctggtc ggcacaaagc aatactcttt ttagcactat
    25801 caacaaacct gccgccattg ttgaaatgac caccaatggt gattttatcc gcacaattcc
    25861 tctggatttt gttaaggatc tcgagacgat cgaatacatt ggcgataatc agtttgtcat
    25921 tagcgatgag cgcgattacg ccatttatgt gatttcactg accccaaact cggaagtaaa
    25981 aatcctcaaa aaaataaaaa tcccgctgca agactcccca accaattgcg gttttgaagg
    26041 gttggcttat tctcgccagg atcacacttt ctggtttttt aaagagaaaa atccgattga
    26101 ggtctacaaa gttaacgggc tgttaagtag caatgaatta cacatcagca aagacgaggc
    26161 actacaacgg caatttactt tagatgatgt gtccggcgca gaatttaacc agcaaaaaaa
    26221 tacgcttctg gtgctatctc atgaatctcg cgcactccag gaagtaaccc tggttggcga
    26281 ggttatcggt gaaatatcac taaccaaagg tagtcgaggt ttgtcgcata acatcaaaca
    26341 ggccgaaggg attgcgatgg atgcctctgg taatatttat atcgttagcg aaccaaatcg
    26401 tttttatcgc tttaccccta aatcatcgca ttaacaggct ggcatatttc acctttttgt
    26461 tattaacttg ttaacaacaa aaatgacata caaggttaaa catgccttcg tcctcgcatc
    26521 ccgtagaacg cttttctttc agcaccgcgc ttttcgggat gctggttctg accttaggta
    26581 tgggattagg tcgcttcctt tatacgccta tgttgcccgt catgatggcg gaaggggcgt
    26641 tttcatttag ccagctctcg tggattgcca gcggcaacta tgcggggtat ctggctggca
    26701 gtctgctatt ttcgtttggc gcatttcacc agccgtcgcg cctgcgccca ttactgttgg
    26761 tttctgccct ggcgagcggg ttgttgatcc tcgcaatggc atggttgccg ccatttattc
    26821 tggtgttatt gattcgcttc ctggcgggtg tcgccagcgc cggtatgctg atttttggtt
    26881 cgacgctgat tatgcagcac acgcgccatc cttttgtgct ggcagctttg ttttctggcg
    26941 ttggcattgg cattgcactg ggcaacgaat atgttctggc aggcctgcat tttgccttct
    27001 cctcgcaaac gttatggcaa ggcgccgggg cgctttctgg catgatgctg attgcactta
    27061 cgcttttaat gccctcgaaa aaacacgcca tcgcaccaat gccattggca aaaacggagc
    27121 aacagataat gagctggtgg ttactggcta ttctgtacgg ccttgcggga tttggttata
    27181 tcatcgtcgc gacttacctg ccgctcatgg cgaaagatgc aggctcacca ttgttaaccg
    27241 cccatctctg gacgttagtc ggcttatcaa ttgtgccagg ctgctttggc tggctatggg
    27301 cagcaaaacg ttggggggct ttgccttgcc tgacggcgaa tttgctggtg caggctatca
    27361 gtgtgctgct tactctcgcc agcgactctc ctctcttgct tatcattagt agtatcggtt
    27421 ttggcggtac ctttatgggc acgacatctc tggtgatgac tatcgcccgc caactgagcg
    27481 ttccgggaaa tctcaatctt ttaggctttg tgacgctcat ttacggcatt ggacaaatcc
    27541 tcggcccggc gctgaccagt atgctcggca atggaacatc ggcgcttgcc ggcgccactc
    27601 tctgcggcgc ggcggcgttg tttattgctg cgcttatcag cacagtacaa ttatttaagc
    27661 tgcaagtggt cacttcatga ggagtgtttc ccttaccact caagaaactc aacagcaacc
    27721 gttgatgtca gccagacccc gttttccgct tgccagaaag ggatgcctcg tttcgccatt
    27781 tcctgtgctt tgacggttaa gataaccggc gatccgtgtc ttgctcccac tttgcgtgcc
    27841 gttgcttcat cagcagagag atgtacgtaa tgacgctccc ctgcaatcag cccctgtttt
    27901 tttatctcgt caaggaaacg gcttgccgta ccgtggtaga gaaactgtgg cggtgttttt
    27961 tcggcaaagg aaatggcgac ctgcgaagtc gaatgcccct gaaccgcgcg gatgcaccta
    28021 ccatcactgg aataactaaa acgcttttta tcgctggtgg caaccacagt atccagcaat
    28081 gcacgggtta accgcttgcc cgctttttgg gcacaaagta tgagcttatc gatatccgcc
    28141 catccttcac ggtcaagtac gataccaatc gcctccggtt tgtgtcggag gacaaaactt
    28201 aaaaacttgc tcgtatccgc caactctttt tcgttgtact ttgccatcgc gttttcgtcc
    28261 ctggattttc tgtttttatt tactgatgtc attgtcactg ggtttatgaa ttttctgcaa
    28321 ctcaatcctg cgcagagcct tttctacaca gcgtaatttt tccgctatgc aaaaaacgca
    28381 tagcaagaaa catacttgca ttttccccat acccacatgg ctgataaggt ttttcagctc
    28441 caggaatttt cctggctcaa gcaacacaca aacacaaaaa aacaatgggg attgttatga
    28501 cacaacaagg ggatgctgta gccggggagc tggcgacaga aaaagtgggc attaaagggt
    28561 atctggcttt ttttctcacc ataatctttt tttccggagt tttttccggg acagacagct
    28621 ggtggcgcgt ttttgatttt tccgttctga acggatcgtt tgggcaactg cctggtgcga
    28681 atggagccac cacctcattt cgcggggcgg gtggcgcggg ggctaaagat ggctttctct
    28741 ttgcgctgga gctggcaccg tcagtcattc tctctctggg gattatttca attaccgatg
    28801 gtcttggtgg attacgcgcc gcgcagcagt taatgacgcc tgtgctaaaa ccgttgttgg
    28861 gcattccagg gatctgctcg ctggcgctga tcgctaacct gcaaaacact gatgccgccg
    28921 ccggtatgac caaagagctg gcgcaggaag gggaaatgac cgaacgcgat aaagtgattt
    28981 tcgccgcata tcaaaccagc ggtagcgcca tcatcaccaa ctacttctct tccggcgtcg
    29041 cggtgttcgc ctttctgggc acgtccgtaa tcgtaccttt agccgtcatc ctggtgttta
    29101 aatttgtcgg agccaacatt ttgcgcgtct ggcttaactt cgaagaacgt cgcaacccaa
    29161 cgcaaggagc gcaagcatga caactcaggt ccgcaaaaac gtcatggata tgtttatcga
    29221 tggcgcgcgc cgtggcttta ctatcgccac gaccaacctg ctgccaaacg tggtgatggc
    29281 gtttgtcatc attcaggcgc tgaaaattac cggcctgctc gactgggtgg ggcatatctg
    29341 tgaacccgta atggcgctgt ggggattacc cggtgaagcg gcaaccgtcc ttttagccgc
    29401 attaatgagt atgggcggcg cagtcggtgt tgccgccagt ctggcgaccg ccggcgcatt
    29461 aaccggtcac gatgtcaccg ttttactgcc cgcgatgtat ttaataggca acccggtgca
    29521 aaatgtcggt cgctgcctgg ggacagccga agtgaatgcc aaatattacc cgcatatcat
    29581 tacggtctgc gtgattaacg cattgctgtc gatctgggtt atgcagctta ttgtttaaca
    29641 aggagttacc atgattgatt ataccgcagc cggttttacc ctgctgcagg gagcgcattt
    29701 gtatgcgccg gaagatcggg gaatttgcga tgtcctcgtc gctaacggca aaattatcgc
    29761 cgttgccagc aatatccctt ctgacattgt accggactgc acggttgtcg atctcagcgg
    29821 gcagatcctc tgtcctggtt ttattgatca acacgtccat ttgattggtg gtggcggcga
    29881 agcaggtccc acgacgcgca cgccggaagt ggcgctaagt cgcctgacgg aagcgggcgt
    29941 cacgtcagtg gttggtctgc tgggcaccga ctctatctct cgccacccgg aatccctgct
    30001 cgccaagacc cgtgcgctca atgaagaagg catcagcgcc tggatgctga ccggcgctta
    30061 tcatgtccct tcccgcacca ttacgggttc cgtggaaaaa gacgtggcga ttatcgatcg
    30121 tgtgattggc gtgaaatgcg ccatctctga tcaccgttct gccgcaccgg acgtttatca
    30181 cctggccaat atggcggcag aatcccgcgt tggcggtttg ctcggcggta aacctggcgt
    30241 caccgtgttc cacatgggcg acagtaaaaa ggcgttacag cctatttatg acctgctgga
    30301 aaactgcgat gtgccgatca gcaagctgct gccgacccac gttaaccgca acgtaccgtt
    30361 gtttgagcag gcgctggagt tcgcgcgcaa aggcggcacc atcgatatca ccagcagcat
    30421 tgacgaaccg gtcgcccctg ccgaaggtat tgcccgcgcc gttcaggcgg gtattccgct
    30481 ggcacgcgtc accctcagct ccgacggcaa cggtagccag ccgttcttcg atgacgaagg
    30541 gaatttaacc catatcggtg ttgcaggttt tgaaacgttg ctggaaaccg tgcaggtgct
    30601 ggtcaaagac tatgatttca gtatcagcga tgccctgcgc ccgctcacca gtagcgtagc
    30661 cggtttcctt aacctgaccg ggaaaggtga aattctgcca ggcaatgatg ctgacttgct
    30721 ggtcatgacg ccagaactgc gcattgagca ggtatacgct cgcggcaaac tgatggtcaa
    30781 agacggcaaa gcctgcgtga aaggaacgtt tgaaacggct taaacggttg tcagccgccc
    30841 gaatggacgt agaatgcccg caagggcggc tgacagagta aaacgtaatg gatgactgtg
    30901 gtgcgatttt gcataatatt gaaaccaaat ggctttatga ttttctgacc ctggaaaaat
    30961 gccgcaattt ttcccaggcg gcagtcagtc gcaacgtctc gcaaccggca ttcagccgcc
    31021 gcatccgtgc gctggaacag gcgattggtg ttgaattgtt taaccgccag gtaacgccgc
    31081 tgcaactctc agaacaaggg aaaatcttcc attcgcagat ccgccatctg ttgcaacagt
    31141 tagagagcaa tctggcagag ctgcgtggcg gcagcgatta cgcgcaacgt aaaatcaaga
    31201 tagccgctgc acactctctt tccctcggtc tgttaccgtc cattatcagc cagatgccgc
    31261 cgctctttac ctgggcaatt gaagccattg atgtcgatga agcggtcgat aaactgcgtg
    31321 aagggcaaag tgactgtatt ttttcctttc acgacgaaga tttgctggaa gcgccgtttg
    31381 accacattcg cttatttgaa tctcaattgt tccctgtctg cgccagtgac gaacacggag
    31441 aagcactttt taacctcgcg cagccacact ttccgttact gaattacagc cgcaactcct
    31501 acatggggcg attgattaat cgcaccctga cgcgccacag tgagttaagt ttcagcacct
    31561 tttttgtctc ttcgatgagc gagcttttaa agcaggttgc cctcgacggc tgtgggattg
    31621 cctggctgcc ggagtacgcc atccaacaag aaattcgcag cgggaagctc gttgtactga
    31681 atcgggacga actggtgatc ccgattcagg cctatgcata ccggatgaat acccgcatga
    31741 atcccgttgc cgaacgcttc tggcgtgaac tgcgcgagct ggagattgtg cttagctgac
    31801 attctccagc gtcgcactgc gccaaccgcc attatcccag accagatcaa acgttagcgg
    31861 ttcatcgtcg tggtacagca ttatctcaag gctgatcgcc agagtgtggc agtacgcttc
    31921 tttcagatta acctgcaacg ccgcaatccg tggctcatga aataacagtg cagaacgcag
    31981 ctgacggcaa taccagctct ggagatgttt gtcgctcggg ttatgcactt gccactcggg
    32041 catgcctgaa acaagtaatg acggtaatgc cgaatgagga gaattaaaaa gagtgaggag
    32101 atcgctgcat accgatttac gcagcaatga cgttttattg cctgaaattt caggtaaaac
    32161 agcctgaagt gattggcgca tcattttgcg cactcctgac gtttagcaac atatgcggct
    32221 tgccctgaac accgggcacc actgtattga tgctcatttt ccatagccat tcccttttgt
    32281 tcattacatc catgtcagtc tgagacaggc ctgaatcctg tcagcaaagt ataaaacctt
    32341 acttgcctat gaatatctac tgacttttca ggaaaaaagt catgatttga taaaaatcat
    32401 caaactcaaa aaaaaaccgc tagtatattc tgaatattcc gaatagttat gtagatgaaa
    32461 atacttgtca ccgccgctac ttaattccgg tgggaaatta ttta
//
LOCUS       DAZFXT010000048        31994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.048, whole genome shotgun sequence.
ACCESSION   DAZFXT010000048 DAZFXT010000000
VERSION     DAZFXT010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.048"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..118
                     /locus_tag="ACHKW0_002357"
     CDS             <1..118
                     /locus_tag="ACHKW0_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7475184.1"
                     /translation="RTAIPFEGERHNALDDARYQAKYVSAIWKKLIPSQADF"
     gene            190..441
                     /locus_tag="ACHKW0_002358"
     CDS             190..441
                     /locus_tag="ACHKW0_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HFO7475185.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLA"
     gene            461..1756
                     /locus_tag="ACHKW0_002359"
     CDS             461..1756
                     /locus_tag="ACHKW0_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO7475186.1"
                     /translation="MREAEMKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRR
                     IAGELRASVCYAIKTGAFDYAKQFPASHNLEKFGEARQDLTIKELAEKFLALKETEVA
                     KTSLNTYRAVIKNILSIIGEKNLASSINKEKLLAVRKELLTGYQIPKSNYIVTQPGRS
                     AVTVNNYMTNLYAVFQFGVDNGYLANNPFKGISPLKESRTIPDPLSREEFIRLIDACR
                     NQQAKNLWCVSVYTGIRPGELCALGWEDIDLKNGTMIIRRNLAKDRFTVPKTQAGTNR
                     VIHLINPAIDALRSQMALTRLSKEHIIDVHLREFGRTEKQKCTFVFQPEVSAKVKNYG
                     DHFTVDSIRQMWDAAVKRAGIRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQM
                     VFQVYGKWMSENNNAQVTLLNTQLSEFAPTMPHNEAMKS"
     gene            complement(1944..2963)
                     /gene="rspB"
                     /locus_tag="ACHKW0_002360"
     CDS             complement(1944..2963)
                     /gene="rspB"
                     /locus_tag="ACHKW0_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HFO7475187.1"
                     /translation="MKSILIEKPNQLAIVEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFTEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(2975..4189)
                     /gene="rspA"
                     /locus_tag="ACHKW0_002361"
     CDS             complement(2975..4189)
                     /gene="rspA"
                     /locus_tag="ACHKW0_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HFO7475188.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(4395..4721)
                     /gene="ynfA"
                     /locus_tag="ACHKW0_002362"
     CDS             complement(4395..4721)
                     /gene="ynfA"
                     /locus_tag="ACHKW0_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HFO7475189.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            4856..5197
                     /gene="ynfB"
                     /locus_tag="ACHKW0_002363"
     CDS             4856..5197
                     /gene="ynfB"
                     /locus_tag="ACHKW0_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HFO7475190.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            5232..5792
                     /gene="speG"
                     /locus_tag="ACHKW0_002364"
     CDS             5232..5792
                     /gene="speG"
                     /locus_tag="ACHKW0_002364"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HFO7475191.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(5795..6505)
                     /gene="ynfC"
                     /locus_tag="ACHKW0_002365"
     CDS             complement(5795..6505)
                     /gene="ynfC"
                     /locus_tag="ACHKW0_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HFO7475192.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VNPAVERVYTIKNTIDYY"
     gene            6613..6918
                     /gene="ynfD"
                     /locus_tag="ACHKW0_002366"
     CDS             6613..6918
                     /gene="ynfD"
                     /locus_tag="ACHKW0_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HFO7475193.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            7117..9543
                     /gene="ynfE"
                     /locus_tag="ACHKW0_002367"
     CDS             7117..9543
                     /gene="ynfE"
                     /locus_tag="ACHKW0_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfE"
                     /protein_id="HFO7475194.1"
                     /translation="MSKNERMVGISRRTLVKSTAIGSLALAAGGFSLPFTLRSAAAAV
                     QQAREKVVWGACSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQVRACLRGRSI
                     RRRINHPDRLNYPMKRVGKRGEGKFERISWDEALDTIASSLKKTVEQYGNEAVYIQYS
                     SGIVGGNMTRSSPSASAVKRLMNCYGGSLNQYGSYSTAQISCAMPYTYGSNDGNSTTD
                     IENSKLVVMFGNNPAETRMSGGGITYLLEKAREKSNAKMIVIDPRYTDTAAGREDEWL
                     PIRPGTDAALVAGIAWVLINENLVDQPFLDKYCVGYDEKTLPADAPKNGHYKAYILGE
                     GDDNTAKTPQWASQITGIPVDRIIKLAREIGTAKPAYICQGWGPQRQANGELTARAIA
                     MLPILTGNVGISGGNSGARESTYTITIERLPVLDNPVKTSISCFSWTDAIDHGPQMTA
                     IRDGVRGKDKLDVPIKFIWNYAGNTLVNQHSDINKTHEILQDESKCEMIVVIENFMTS
                     SAKYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKR
                     LGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYK
                     AFRDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWDSPER
                     STFPLQLFGFHYKSRTHSTYGNIDVLKAACRQEVWINPIDAQKRGIANGDMVRVFNHR
                     GEVRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQ
                     HTNLVEIEKI"
     gene            9604..12027
                     /gene="ynfF"
                     /locus_tag="ACHKW0_002368"
     CDS             9604..12027
                     /gene="ynfF"
                     /locus_tag="ACHKW0_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate/tellurate reductase subunit YnfF"
                     /protein_id="HFO7475195.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGKEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            12038..12655
                     /gene="ynfG"
                     /locus_tag="ACHKW0_002369"
     CDS             12038..12655
                     /gene="ynfG"
                     /locus_tag="ACHKW0_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMSO reductase like molybdoenzyme iron-sulfur
                     subunit YnfG"
                     /protein_id="HFO7475196.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            12657..13511
                     /gene="ynfH"
                     /locus_tag="ACHKW0_002370"
     CDS             12657..13511
                     /gene="ynfH"
                     /locus_tag="ACHKW0_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenate reductase membrane anchor subunit YnfH"
                     /protein_id="HFO7475197.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLACIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWVCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            13554..14168
                     /gene="dmsD"
                     /locus_tag="ACHKW0_002371"
     CDS             13554..14168
                     /gene="dmsD"
                     /locus_tag="ACHKW0_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HFO7475198.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            14327..15619
                     /gene="clcB"
                     /locus_tag="ACHKW0_002372"
     CDS             14327..15619
                     /gene="clcB"
                     /locus_tag="ACHKW0_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HFO7475199.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(15572..16267)
                     /gene="bioD"
                     /locus_tag="ACHKW0_002373"
     CDS             complement(15572..16267)
                     /gene="bioD"
                     /locus_tag="ACHKW0_002373"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HFO7475200.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(16392..17612)
                     /gene="mlc"
                     /locus_tag="ACHKW0_002374"
                     /gene_synonym="dgsA"
     CDS             complement(16392..17612)
                     /gene="mlc"
                     /locus_tag="ACHKW0_002374"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar metabolism global transcriptional
                     regulator Mlc"
                     /protein_id="HFO7475201.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLLGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            complement(17747..18640)
                     /gene="ynfL"
                     /locus_tag="ACHKW0_002375"
     CDS             complement(17747..18640)
                     /gene="ynfL"
                     /locus_tag="ACHKW0_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7475202.1"
                     /translation="MNIELRHLRYFVAIAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYEDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            18747..20000
                     /gene="ynfM"
                     /locus_tag="ACHKW0_002376"
     CDS             18747..20000
                     /gene="ynfM"
                     /locus_tag="ACHKW0_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7475203.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            complement(20049..20168)
                     /locus_tag="ACHKW0_002377"
     CDS             complement(20049..20168)
                     /locus_tag="ACHKW0_002377"
                     /inference="COORDINATES: protein motif:HMM:NF033695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_01571 family protein"
                     /protein_id="HFO7475204.1"
                     /translation="MTHSKMNPFIWVLIILMTLDALRELAGASSILGWLLTLV"
     gene            20424..20732
                     /gene="asr"
                     /locus_tag="ACHKW0_002378"
     CDS             20424..20732
                     /gene="asr"
                     /locus_tag="ACHKW0_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance repetitive basic protein Asr"
                     /protein_id="HFO7475205.1"
                     /translation="MKKVLALVVAAAMGLSSAAFAAETANTPAPTATTTKAAPAKTTH
                     HKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA
                     "
     gene            20825..20908
                     /locus_tag="ACHKW0_002379"
     CDS             20825..20908
                     /locus_tag="ACHKW0_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475206.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            21008..21829
                     /gene="ydgD"
                     /locus_tag="ACHKW0_002380"
     CDS             21008..21829
                     /gene="ydgD"
                     /locus_tag="ACHKW0_002380"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HFO7475207.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYDIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(21868..22197)
                     /gene="mdtI"
                     /locus_tag="ACHKW0_002381"
     CDS             complement(21868..22197)
                     /gene="mdtI"
                     /locus_tag="ACHKW0_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HFO7475208.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(22184..22549)
                     /gene="mdtJ"
                     /locus_tag="ACHKW0_002382"
     CDS             complement(22184..22549)
                     /gene="mdtJ"
                     /locus_tag="ACHKW0_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015606 - spermidine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015848 - spermidine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HFO7475209.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVMLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            22961..23104
                     /locus_tag="ACHKW0_002383"
                     /pseudo
     CDS             22961..23104
                     /locus_tag="ACHKW0_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pheromone autoinducer 2 transporter"
     gene            complement(23615..23896)
                     /locus_tag="ACHKW0_002384"
     CDS             complement(23615..23896)
                     /locus_tag="ACHKW0_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475210.1"
                     /translation="MTEAEILRLIRRVSGISQQHDEQATQPDSVTAENYARVVAEVMR
                     RDGIQLNDVDMRNIRIRVLEMLAYRRRVQTYREKAKITYHWKKPERLRT"
     gene            complement(23910..25571)
                     /locus_tag="ACHKW0_002385"
     CDS             complement(23910..25571)
                     /locus_tag="ACHKW0_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7475211.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLENSLYMFDREV
                     VERFIVFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDDARQMCLLSRPLRKRVNI
                     QAHKVMHPKSNSLLKPLAAKAATIEGTNPSLAIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPEMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGATPWMGNGAWAECAGK
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKNIMLVSRHYLPEFQLQNPANKNRA
                     IYRQWARAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(25555..25911)
                     /locus_tag="ACHKW0_002386"
     CDS             complement(25555..25911)
                     /locus_tag="ACHKW0_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HFO7475212.1"
                     /translation="MARPPKAPAYLDDIAVKQWREKSRQLAERGDLTPADWSNLELYC
                     VNYSIYRKAVADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRR
                     KNPPETEEEDELDRLE"
     gene            complement(26035..26217)
                     /locus_tag="ACHKW0_002387"
     CDS             complement(26035..26217)
                     /locus_tag="ACHKW0_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475213.1"
                     /translation="MALMNEREQNRLIRGLIRQRDAWKAQEAGHKDKASGRAERITAT
                     RLNDRDREVMECFRNR"
     gene            complement(26201..26641)
                     /locus_tag="ACHKW0_002388"
     CDS             complement(26201..26641)
                     /locus_tag="ACHKW0_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HFO7475214.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRSARQQQDSRRGSSRER
                     GYTRQWEKYRAMYLSKNPLCAHCLEKGIYTPAVVVDHIIPIDGGNDVLFWPEWNHQPL
                     CQACHNQKTKWLDPSTKNKRAAGGFREEEERGANRNNWMYGADE"
     gene            complement(26641..26934)
                     /locus_tag="ACHKW0_002389"
     CDS             complement(26641..26934)
                     /locus_tag="ACHKW0_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO7475215.1"
                     /translation="MTELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRRF
                     DDGLEFTPAIKIGCMMFIAHLYENRQIVADNAKTHVPMTIGALWTAYRDVGVY"
     gene            complement(26931..27269)
                     /locus_tag="ACHKW0_002390"
     CDS             complement(26931..27269)
                     /locus_tag="ACHKW0_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7475216.1"
                     /translation="MNIGRLRDRVTIQTLKQTRAMTGEILETWVDGHTLWASVNMISS
                     KEAISSGAELATGTVRIWIRYRKDINATSRIKVNTGPLAGRVLNIIGQPLPDAARTRL
                     EILCSEGAEK"
     gene            complement(27266..28477)
                     /locus_tag="ACHKW0_002391"
     CDS             complement(27266..28477)
                     /locus_tag="ACHKW0_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7475217.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISEAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRHCLLNGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGEHNWRYCITDAYTGNTRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSGVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(28479..29036)
                     /locus_tag="ACHKW0_002392"
     CDS             complement(28479..29036)
                     /locus_tag="ACHKW0_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO7475218.1"
                     /translation="MKSMEIRSSEITTSASNTLTGYVVRWDNLSELLWGEFYEKFQRG
                     AFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDDTGLRFELTPPDTSTGRDVIE
                     LVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGVELARR
                     SLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(29092..30249)
                     /locus_tag="ACHKW0_002393"
     CDS             complement(29092..30249)
                     /locus_tag="ACHKW0_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7475219.1"
                     /translation="MPRIIELRQQKTAIKNQMRDMLENAEKENRSLNDAEGAKFDELR
                     AKAESLDKDISRLEAIADEERSKPGKSSQTTDPAELRNYILTGETRALSTGVPADGGY
                     TVIPELNTEIMRMLADESTMRRICTVKKISSNEFKQLVSAGGATVNHGEEGKAREQTS
                     TPQINEVSIKLYPVYAYPRTTQEIVDFSDVDILSWLTGEIGDTFTETEESDLVVGDGD
                     KKAKGFLSVPRAEKNDKERDFGTLQVIKPSESLAWTSADPLIDLKFALRKKYRKNAVW
                     VVNSTTAAKLQKVKNANGDYIWRDRLQAGDPDTLLGLPVEYLEFMPDNVIALGDFKRG
                     YYIVDHETGVRTRPDNLTEPGFIKIFTQKYLGGGVVDSNAIKILELPQDDD"
     gene            complement(30541..30765)
                     /locus_tag="ACHKW0_002394"
     CDS             complement(30541..30765)
                     /locus_tag="ACHKW0_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309616.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475220.1"
                     /translation="MPMKFDEILKQRDKYHADNMETMNINDYRAFLETGALIEKDRHG
                     FVRCALSGEMLAVNPEQIDALIEFLKGIRD"
     gene            complement(30891..31163)
                     /locus_tag="ACHKW0_002395"
     CDS             complement(30891..31163)
                     /locus_tag="ACHKW0_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7475221.1"
                     /translation="MVHDRIAEELEAKGFYRRAAARWGEVMMLVETDKERHHITMRRL
                     ECSRKAQRPPEPPDNFGDLRNAVNRTCAEMGIDGAGDEIRRNYQDS"
     gene            complement(31174..31584)
                     /locus_tag="ACHKW0_002396"
     CDS             complement(31174..31584)
                     /locus_tag="ACHKW0_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7475222.1"
                     /translation="MTAQIAAYGRLVDDPQVKQTSKGTPMTLARMAVSLPCSQAQDGQ
                     ATLWLSVLAFGKQADQLAGHEKGNVISVSGTMQISQWTGQNGETRQGYQVIADSVISA
                     KTVRPGGSRRKTTGTQGNQPPAGGDDPYGDDIPF"
     gene            complement(31594..31899)
                     /locus_tag="ACHKW0_002397"
     CDS             complement(31594..31899)
                     /locus_tag="ACHKW0_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475223.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWA
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(31896..>31994)
                     /locus_tag="ACHKW0_002398"
     CDS             complement(31896..>31994)
                     /locus_tag="ACHKW0_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475224.1"
                     /translation="TAAANFATLTEREIAEAEQVTTINVKTGEVES"
BASE COUNT         7822 a         8278 c         7712 g         8182 t
ORIGIN      
        1 cagaactgct atcccattcg aaggtgagcg ccataatgca cttgatgacg cccgttatca
       61 ggcaaaatac gtttcagcta tctggaaaaa actgatcccg agtcaggctg atttttaatg
      121 ttcaaccgtc gtcggttgca attggtattc tgcaaccggc gttcatctga tgtaagagat
      181 aaagaatcga tgagcgaagt aatcatgatc gtctctcctg gcaaatgggt atccgaagag
      241 cagttaattg cacttaaagg aatcaaaaag ggaacgctaa aaaaagccag ggaaaaatcg
      301 tttatggaag gaagggaata taagcatgtc gctcatgacg gtatgccatg ggataacagt
      361 ccatgctttt acaacctgga agaaattgat cgctggattg agcgccaggc atctgcaaga
      421 ccaagacgtc atcttgcttg actaaaagca acacgaacca atgagagaag ctgaaatgaa
      481 atatccgaca ggcgtggaaa accatggagg gaaattacgt atctggtttg tttataaagg
      541 tgtaagagtc cgggaaaatc ttggggttcc tgacacagca aaaaacaggc gcattgcagg
      601 tgagctacgc gcctctgttt gttacgcaat aaaaactggc gctttcgact atgcaaaaca
      661 gtttcccgcc tcacacaatc tggaaaaatt tggtgaggcc cgacaagatt taaccataaa
      721 agaactggct gaaaaatttc tggcactgaa agaaactgaa gtcgcaaaaa cgtcactcaa
      781 cacgtaccgt gccgtcatca aaaatattct gagcataatc ggtgaaaaaa atcttgcatc
      841 atcgattaat aaagaaaaat tgctggcggt acgtaaagag ttactgactg gataccagat
      901 ccccaaaagt aactatattg ttacacaacc agggagatcg gctgttaccg taaataatta
      961 catgacaaat ctttacgccg tgttccagtt tggtgttgat aacggttatc tggcaaacaa
     1021 tccatttaag gggatctcac cattaaagga gtcgagaacc atcccggatc cactttcgcg
     1081 ggaggagttt atccgcctta ttgacgcgtg cagaaatcag caagccaaaa atttatggtg
     1141 tgtttccgtt tatactggga ttcgccctgg tgaactgtgt gcgcttggat gggaagatat
     1201 agatctgaaa aatggaacaa tgataatcag aagaaatctg gcaaaagacc gttttacagt
     1261 accaaaaaca caggcgggaa ccaatcgtgt gattcacctt attaatcccg caatcgacgc
     1321 tctccggagt cagatggcac taacgagact gagcaaagag catatcatcg atgttcacct
     1381 cagagaattc ggcagaacag agaaacaaaa atgtaccttt gtttttcaac ctgaagtgtc
     1441 agcgaaagta aaaaattatg gtgaccattt taccgttgac tcaataaggc agatgtggga
     1501 cgcagcggta aagcgtgccg gaatccgcca tcgcaaatcg tatcagtcga gacacactta
     1561 tgcctgctgg tcgttaacag caggagctaa cccggcattc attgcaaacc agatgggcca
     1621 tgcagatgcg caaatggtgt ttcaggtgta cgggaaatgg atgtcagaaa ataataatgc
     1681 gcaggtaacg ctgttaaata cacagttaag cgagtttgcc ccaaccatgc cccataacga
     1741 agcgatgaaa agttaatttt ttatttatca attagttaga ttgaatgact cttgaaatcc
     1801 ataaattcaa gcgcagtgcc cagccatccc gatactgctg ctttcaccaa atccttagtg
     1861 cttctttcgt gtttttctat tgtcataatg gttatctcta aaaaagaggt aagatgcgta
     1921 ctacttactc gccgttattg gtattattca gaaaaagtga gtaagacttt gcagcaatgt
     1981 ttttgatcct gttcaaataa actaatggca tcagcaacat gctggaaatc aaacgtatgg
     2041 gtaattaatt tttctggttt aattaaccct ttacttaacc agtcgataac gaccgggaat
     2101 ttatttgcat ttaagcgtga agaaaaaata gagagttctt ttccggtaat tccttgctga
     2161 atcacttcag acggttcact ggagaagccc atcaatacaa tacgtgccgc tggagaagcc
     2221 agcgttacgg cctctttcag gatagaagga tgacaagccg catcgataat taatgtcggc
     2281 ttgatgcctt tttcagtgaa aatctcgcca agcggtgtct ggctgttatt aatcgcccag
     2341 tcagccccgc tctctttcgc tttttccagt cgttcatcaa tgcgatcggc aacaatcaca
     2401 tttttaacgt tatagacgcc ttttaatacc tgaacgatcg tcaggccgat tggaccggca
     2461 ccataaacca gaacggtatc attttcagtc ggttgaccat gtccggttac gttagccgca
     2521 atggtaaaag gttcgatcat taccgcatat tgatcggcca ctgcttcagg aattttccac
     2581 gcattttttg ccggaaccac ggcatattca ctgaaaccac cgtcagcgtg cacacctaat
     2641 acagccagtg tcgtacaaac gttcggttta cctatagagc acggatagca atgcccacag
     2701 ctgaccaccg gatcgacagc aacacgttca ccgactctgg cgctttccac gccttcaccc
     2761 actgcatcaa tgacgccaaa gaattcatga ccaatgacgc gcggatattt cgcaaaagga
     2821 ttatgcccac gataaatatg gctatctgaa ccacaaattc cggcaagttt cacttttact
     2881 cgcacttcac ccgctgacgg ggtgggtatt tcacgttcga caatcgccag ttgattcggt
     2941 ttttcaatta atatgctttt cattatctta ctccttacca gttccacagc gtgccatctt
     3001 ccagacgtgc gactggtaga taagcaggtt catagggata tttcgccgcc agcttttcat
     3061 cgaattcgat accaagaccc ggtttgtctc ccggatgcat atagccgtta tcgaaagtcc
     3121 agttgtgcgg gaagacttcg agcatttgtt cggaataacc catgtattcc tggacaccga
     3181 aattggggac ccacagatca aagtgcagcg ccgcagccat gcagactggt gacaaatcgg
     3241 aaggaccgtg tgagccagta cgtacctgat acagcgaagc aaaatcggca atccggcgca
     3301 taccggtaat tccgcctgca tgggtcagcg tggtgcggat ataatcgatg agttgctctt
     3361 caatcagttg tttgcagtcc cagatgctgt tgaagacttc acccactgcg atgggtgtga
     3421 cggtatgttg gcgaatgaga cggaagcatt cctggttttc cgcaggtgtc gggtcttcca
     3481 tccagaacat gcgataatct tcaatgcttt taccaaagcg cgccgcttca ataggcgtta
     3541 agcgatggtg catgtcatgc agcaaatgtt cattaaaacc aaacttgtta cgtaccgcgt
     3601 caaacaattt cggcatgaaa tcgaggtatt tctccgtcga ccacagctgc tcttccggcc
     3661 actgtccttt ggttgcgggt tcataagcca gacctttacc tttcgacatg ccgtaggtgg
     3721 ttttcatacc agggattccg cactgcacgc ggatggcttt gaatcccagc tcctgatgac
     3781 gggcataatc atccagagct tcatcaatac tgtgaccggt ggtatggcaa taaaccatca
     3841 ccccttcacg agacgcgccg ccgagtaact ggtaaagcgg catgttggcc gctttggctt
     3901 taatatccca cagcgccata tcaaccgctg aaatggccga catcgtaacc ggaccgcgac
     3961 gccagtaagc acctttatag aaaaactgcc agatatcttc gatacggtgc gcatcgcgac
     4021 caataagctg cggacaaagg tgatcctgca aataagaggc cacggaaagc tcacgtccat
     4081 tgagagtggc atccccaagg cccgtaatac cgtcctcagt ggtgattttt aatgtgacga
     4141 aattacgccc cggacaggta acaaaaactt cagcctttac gatcttcatg ttcgattcct
     4201 tgcatcgctt gtcgtgatgc atgaaatcta cgcaactgag ctactaccat acaagtatga
     4261 ggatcgaaaa aagccggagt gatcacaaaa aaaggcgtat atttgcgctg tgaatggttg
     4321 acaaaagatg aaatagaata ccttttgtca gctgacactt cctcgtatct tattgataaa
     4381 atggatttat gttcctacgt gcgcccccag cccgcaacaa tgatcaacat gccgcaaagc
     4441 gcaatcaacg cacccgtcca gtcataaaga gtcagtttca cgccatccac aacgcgcagc
     4501 cacatcaacg ccgtgcagac ataaacgcca ccataagccg cgtaaacacg cccactcgcc
     4561 gcaggatgca acgttaacaa ccagacaaac agcgccagtg aaatccccgc cggaagcaac
     4621 agccagatac tggcgtttcg ttttaaccac aaccagggca gaaagcatcc aataatttca
     4681 cacagcgcag tagcaaaaaa tagtaacgtt gttttaatca tctttgtcac ttattgacat
     4741 catgtatagt tatagggcca cataatatca ccaatataaa caccctcctg atacgttttg
     4801 cgttcgcagt ggatggtgta gaatcacctt taatcattca tacagggaat gaattatgaa
     4861 aatcactctc agcaaacgaa tcggcctgct cgcttttctg ctaccttgcg cactggcatt
     4921 gagcacaact gttcatgccg aaactaacaa actggtgatt gagtctggcg acagtgcaca
     4981 aagccgccag cacgccgcta tggaaaaaga gcaatggaat gacacgcgca atctgcgcca
     5041 gaaagtgaat aaacgcactg aaaaagagtg ggataaagcc gacgccgctt ttgataaccg
     5101 cgataaatgt gagcaaagcg ccaacatcaa tgcctactgg gagccaaata ctttgcgctg
     5161 cctggaccgt cgaactggcc gcgttattac cccctaacct gttattgatt taaggaatgt
     5221 aaggacacgt tatgccaagc gcccacagtg ttaagctacg cccgctggag cgtgaagatt
     5281 tacgctatgt acatcaactc gacaataacg ccagtgtgat gcgttactgg tttgaggaac
     5341 cctacgaagc ctttgttgaa ctctctgatc tgtatgataa gcatattcac gatcagagcg
     5401 aacggcgctt tgtggtggaa tgtgacggcg aaaaagccgg tctggtggag ctggtggaaa
     5461 ttaaccatgt tcatcgccgc gcagaatttc agataattat ctccccggag tatcagggga
     5521 aaggtctggc aacccgtgcc gcaaaattag caatggacta tggctttacc gttctcaatc
     5581 tctataagct gtatctgatc gttgataaag agaatgaaaa agcgattcat atttaccgca
     5641 agcttggctt ttcggttgaa ggtgaattga tgcacgagtt ctttattaat ggtcaatatc
     5701 gtaatgccat tcgcatgtgt atattccagc atcagtatct ggcagagcac aaaacaccgg
     5761 gtcagactct cctgaagccg accgcacaat agcattaata ataatcgatc gtatttttga
     5821 tggtgtaaac ccgttcgacg gcggggttta ctccttcatc aacaatgatc agctgacagt
     5881 ccaccggatt ggcgtgactg tcatattcac agctctgttt tacattacca actcgttgat
     5941 tattcagtaa agtaactgct gtataatcta attttttgat cgggtccgtt gatggcgtgg
     6001 cgctgacaga taatgttttg tcgttacttt tcgtggtttt acccagcgga tacccctgat
     6061 catcatagcg atattccatc tgcatttgtt tgctgctggc tttaatcacg aagccattat
     6121 catcggtttc ccaactcacc ccggcagaag gtaattctgc tagctggcat tttccctgta
     6181 aacgtactct tttctccagc gtctcagcat cacggtaata attggcgtcc agtaccagag
     6241 cgaccacggt attattttcc agatccagta attcgagtga atcaaaacag ccttcttccg
     6301 acaaagtccc agaaacacgt ttcgtcactt caccttgctc atccattaat gtctgagtga
     6361 aatcttttac cggaccacgc agcggatcaa aatcgaattc attagagaaa ctggccatct
     6421 caggggtaaa tgaaagtgtt acctctgtgc ggtcacatcc tgtgaggaat atcgcgagta
     6481 agcatggtag taatttgtat ttcacaacag tcaccagaga gtagagatga ttctcaatca
     6541 tagtagcaaa tacagtactt tacacgttaa atgctatgct taaagaagtt atcttcgcgt
     6601 aaggagctta tgatgaaact ctcaacctgc tgtgccgcgc ttctgctcgc cctcgcctca
     6661 cccgcggtac tcgccgcgcc gggatcctgt gagcgcatac agagcgatat atcacagcgc
     6721 attatcaata atggcgtacc cgaaagcagc ttcacgttaa gtattgtacc caatgaccag
     6781 gttgatcagc ctgattccca ggtcgtcggg cattgtgcta atgatacgca taaaattctc
     6841 tacacccgca ccaccagcgg taacgtctct gctcccgcgc agtctaccca ggatggcgcg
     6901 cctgccgaac cgcagtaaca cattatcgac tgaacgccgg atacgacaca tccggcgatt
     6961 tgaacataca acataatccc accttattac tcataccctt ctattgatat ggattaataa
     7021 ttcttaaccc aaaatgggta gactccctct attgttagcg cgctaaatat tcaatatata
     7081 aacttttata taacgataaa gaacagggag tgagttatgt ccaaaaatga acgaatggtg
     7141 ggcatcagcc gcagaacact cgttaaatct accgcgatag gttctctggc gctggctgca
     7201 ggcggttttt ctttgccgtt taccctgcgc agtgcagcag cagcggtaca acaggcccgc
     7261 gaaaaagtgg tctggggtgc ctgttccgtc aactgtggta gccgctgtgc acttcgtcta
     7321 catgttaaag ataatgaagt gacctgggtg gaaactgaca ataccggcag cgatgagtac
     7381 ggcaaccatc aggtacgcgc ctgtttgcgc ggtcgctcca tccgtcggcg tattaatcat
     7441 cccgatcgct tgaattatcc aatgaaacgc gtgggcaaac gcggcgaagg caaattcgaa
     7501 cggattagct gggatgaagc cctggatact atcgccagta gcctgaaaaa aaccgtcgaa
     7561 caatatggca atgaggctgt atatattcag tactcttcgg ggattgttgg cggcaatatg
     7621 acccgctctt cgccatcagc ctcggcggtc aaacgcctga tgaactgcta cggcggttca
     7681 ctcaatcagt atggctccta cagcaccgcg caaatttcct gtgccatgcc ctacacctac
     7741 ggcagtaatg atggcaacag caccacggat attgaaaaca gcaagctggt cgtgatgttt
     7801 ggtaacaacc cggcagaaac ccgcatgagc ggcggtggca tcacttatct tcttgaaaaa
     7861 gcgcgcgaga aatcgaacgc caaaatgatt gttatcgatc cgcgatatac cgatacggct
     7921 gctggtcgtg aagatgaatg gcttcctatt cgcccgggca ccgatgccgc gctggttgcg
     7981 ggtattgcct gggtattgat taacgaaaat ctcgttgatc aaccttttct cgataaatac
     8041 tgcgtcggct atgacgaaaa aaccttaccc gcagatgcac caaaaaatgg tcactataaa
     8101 gcctatattc ttggtgaagg tgatgataac acagcgaaaa cgccgcagtg ggcttcgcaa
     8161 attaccggta tcccggtgga ccgtatcatc aaactggcgc gtgaaattgg cacagcaaaa
     8221 cccgcttata tctgccaggg ctgggggccg caacgccagg caaacggcga gctgactgca
     8281 cgcgctattg ctatgttgcc tattttgacg ggcaatgtcg gcatcagcgg cggaaatagt
     8341 ggcgcgcgag aatcgaccta taccattacc atagaacgcc tgccggtgct ggataatccg
     8401 gtcaaaacgt caatctcctg cttcagctgg acggatgcta tcgatcatgg tccgcaaatg
     8461 acggcaatcc gcgacggcgt ccgcggcaaa gataaactgg atgtgcccat taagttcatc
     8521 tggaactacg cgggaaatac cctggttaat cagcattctg acatcaacaa aacgcatgaa
     8581 attctgcaag acgaatcgaa atgcgaaatg attgtggtca tcgaaaactt tatgacctca
     8641 tcggcaaaat atgccgacat tctgctgcca gacctgatga ccgttgagca ggaagatatt
     8701 attcctaacg actacgccgg taacatggga tatctcattt tcctccagcc agtcaccagc
     8761 gaaaaattcg aacgtaaacc gatttactgg atcctgagtg aagtcgcgaa acgtcttgga
     8821 cctgacgtct atcaaaagtt cacagaaggt cgcacgcagg aacaatggtt acaacatctg
     8881 tacgccaaaa tgcttgccaa agatccagcg ttaccgtctt acgacgaact gaaaaaaatg
     8941 ggtatctata agcgtaaaga tcccaatggt cattttgtcg cctacaaagc atttcgtgac
     9001 gaccccgagg caaatccact taaaacgcct tccggtaaga ttgaaattta ttccagcagg
     9061 ctggcggaaa ttgcccgtac ctgggaactg gaaaaagatg aagtgataag cccattgcca
     9121 gtttatgcct caacctttga aggctgggac tcccctgaac gtagtacctt cccactgcaa
     9181 ctgtttggtt tccattacaa atcccgtact cactcgacct acggcaacat tgatgtcctg
     9241 aaggctgcct gccgtcagga ggtgtggatc aaccctatag atgcgcagaa acgtgggatt
     9301 gccaacggcg atatggtgcg ggtgtttaac catcgtggcg aagttcggct accagccaaa
     9361 gtgacaccac gcattctccc tggggttagc gctatgggcc agggagcctg gcacgaggcc
     9421 aatatgtctg gcgataaaat cgaccatggc ggctgtgtga atacgctaac cactctgcgc
     9481 ccttcaccac tggcgaaggg aaacccgcag cacactaatc tggtcgagat cgaaaaaata
     9541 taacccacta caaccataaa ttctggcatg acattttgtt tgaaaagcaa taagtgagta
     9601 atgatgaaaa tccataccac agaggcgctc atgaaggctg aaatcagccg tagaagtctg
     9661 atgaaaacct ccgcacttgg cagtcttgcg ctggcaagca gtgctttcac tctgccattt
     9721 tcccaaatgg tccgggcggc agaggctccg gtagaagaga aagcggtctg gagttcctgc
     9781 accgttaact gcgggagccg ctgtctgtta cgtttgcatg tgaaagatga caccgtgtac
     9841 tgggtggagt ctgatacgac aggtgacgac gtctacggta atcatcaggt tcgcgcgtgt
     9901 ttacgcgggc gctctatccg ccgacggatg aatcatcctg ataggttgaa atatcccatg
     9961 aagcgcgtcg gcaagcgcgg tgaaggtaaa tttgaacgga taagttggga cgaagccctg
    10021 gataccatca gtgataatct tcggcggatc ctaaaagatt acggcaatga ggctgtacat
    10081 gtcctgtacg gaacaggcgt agatggcgga aacatcacca actcaaacgt cccgtaccgt
    10141 ctgatgaact cttgcggtgg ttttctcagt cgctatggca gctacagtac cgcacagatc
    10201 agtgccgcaa tgagttatat gttcggtgcc aatgatggca acagcccgga tgatatcgcc
    10261 aatacgaaac tggtcgttat gttcggaaat aacccggcag aaacgcggat gagcggcggt
    10321 ggtgtcactt actacgtcga gcaagcccgc gaacgttcaa acgcacgcat gatcgtcatc
    10381 gatccacgtt ataacgacac agctgccggg cgtgaagatg aatggctgcc cattcgccca
    10441 ggcaccgatg gcgcactggc ctgtgcgatt gcctgggtac tgattactga aaacatggtc
    10501 gatcagccat ttctcgacaa atattgtgtt ggttacgatg aaaaaacgct gcccgccaac
    10561 gcaccacgta acgcgcatta taaagcctat attctgggcg aagggcctga cggcatagct
    10621 aaaacgccgg aatgggcagc aaaaatcacc agcatcccgg cagaaaaaat tatccagttg
    10681 gcacgagaga tcggttcagc aaaacctgct tatatttgtc agggttgggg gccacaacga
    10741 cattccaacg gcgagcaaac gtcccgcgct attgccatgc tttccgttct caccggtaac
    10801 gtcggcataa acggcggcaa ctcaggtgta cgcgaaggta gctgggatct gggggtagaa
    10861 tggttcccga tgcttgagaa ccctgttaaa acgcagattt ccgtctttac atggacagat
    10921 gccatcgacc atggtaagga aatgaccgcg acccgtgatg gtgttcgtgg aaaagaaaaa
    10981 ctggatgtcc ccatcaagtt tttatggtgc tacgccagta acacattgat caatcaacat
    11041 ggcgacatca atcacaccca tgaggtgctt caggatgaca gcaagtgcga gatgattgtt
    11101 ggcattgacc acttcatgac ggcctcggct aagtattgcg atatcctgtt gcccgacctg
    11161 atgccaacag agcaagaaga ccttatctct catgaatctg cagggaatat gggctatgtg
    11221 atcctcgccc aacccgcaac ctctgcaaaa tttgaacgca aacccatcta ctggatgctg
    11281 agtgaagtcg ccaaacgctt aggaccagac gtttatcaaa cctttactga aggtcgcagt
    11341 cagcatgaat ggatcaaata tctccatgcg aaaacgaagg aacgtaaccc tgagatgccc
    11401 gactacgagg agatgaaaac gaccgggatc tttaagaaaa aatgcccgga agaacactac
    11461 gtcgctttcc gcgcattccg tgaagatccc caggcaaacc cgttgaaaac accttcgggg
    11521 aaaatcgaaa tttattctga acgactggcg aagattgcgg atacctggga attgaaaaaa
    11581 gatgaaatta ttcatcccct tccagcttat acgccaggtt ttgatggctg ggacgatccc
    11641 ctgcggaaaa cctatccact gcagttaacg ggcttccact acaaggcgcg tacccactcc
    11701 agctacggca atattgatgt gttacagcag gcctgcccac aagaggtgtg gatcaacccc
    11761 attgatgctc aggctcgcgg tatccgtcat ggcgataccg tgcgggtatt taacaataat
    11821 ggagaaatgc tgattgccgc aaaagtgact ccgcgtattc tgcctggcgt taccgccatc
    11881 gggcaaggtg cgtggcttaa agcggatatg tttggtgacc gggtcgatca cggcggcagt
    11941 atcaatattc tgacctctca ccgcccttca ccgctggcaa agggaaaccc gtcgcacagc
    12001 aatcttgtcc agatcgaaaa ggtttaaaga gtagcccatg accacacaat atggattttt
    12061 tatcgattcc agccgctgta ctggctgtaa aacctgcgaa ctggcgtgca aagattttaa
    12121 agatcttggc ccggaagtca gtttccgccg tatttatgaa tatgctggcg gcgactggca
    12181 ggaagataat ggcgtctggc accagaacgt gttcgcttat tatctctcca tctcctgtaa
    12241 ccattgcgat gacccagcat gtacaaaagt ctgccccagc ggcgcaatgc ataagcgtga
    12301 agatggcttt gtggtggttg atgaagatgt ttgtatcggc tgccgctact gccacatggc
    12361 ttgtccgtac ggcgcaccac agtacaatgc tgaaaaaggg cacatgacga agtgcgatgg
    12421 ttgttattcg cgcgtcgccg aggggaaaca acccatatgc gtcgaatcct gcccgctgcg
    12481 tgcacttgag ttcggtccaa ttgaagagct gcgccagaag cacggcactc tggctgccgt
    12541 cgcgccactg ccgcgcgcgc atttcacaaa acccaatatc gttatcaaac ctaacgccaa
    12601 cagccgcccg accggcgata ccactggtta tctggctaat ccggaggaag tgtaaaatgg
    12661 gaaatggatg gcatgaatgg ccactggtta tctttaccgt attgggtcaa tgcgtagtag
    12721 gcgcgctgat tgttagcgga attggctggt tcgccgccaa aaatgacgca gaccgtcagc
    12781 gtattgtccg tggcatgttt ttcctctggc tgttgatggg catcgggttt attgcctctg
    12841 tcatgcacct gggatcgcca ctgcgagcgt ttaactcgct gaatcgaatc ggagcttctg
    12901 gtctgagtaa cgagattgct gccggttcca tattttttgc cgttggcgga ctatggtggc
    12961 tggtggcggt catcggtaaa atgccgcaag cgttggggaa actctggtta cttgtcagca
    13021 tggcgctcgg cgtcattttc gtctggatga tgacctgcgt gtatcaaatc gacaccgtgc
    13081 caacctggca taacggttac actacgctgg ctttcttcct gacggtattg ttgagcggtc
    13141 caatacttgc tgccgcaatt ctgcgggcag ctcgcgttac atttaatacc acaccatttg
    13201 ccatcattag cgttctggcg ctgatcgctt gtgccggggt gattgtgctg caaggtttgt
    13261 ctctggcgtg tattcactca tccgtacaac aagccagcgc actggtacca gattacgcct
    13321 ccttacaggt ctggcgtgtg gtattgttat gcgcaggtct aggatgctgg gtttgtccgc
    13381 tcattcgtcg ccgtgaacct cacgttgccg gacttattct ggggttaatc ctcattcttg
    13441 gcggtgagat gatcgggcgt gtgctctttt atggcctgca catgacggta ggaatggcta
    13501 ttgcaggtta acaaattggt gcggggcaac ccgcacattt caggatgtta ggaatgaccc
    13561 atttttcaca gcaagataat ttttctgtcg cggcgcgcgt gttgggcgcg ctgttttatt
    13621 acgctccaga gagtgcggaa gccgcacctc tggttgcggt actcaccagt gacggctggg
    13681 aaactcagtg gcctctacca gaagcgtcat tagcgccgct ggtgacggct tttcagacac
    13741 agtgtgaaga gactcacgcc caggcctggc agcgtttgtt tgtcggcccg tgggcactgc
    13801 cgtctccgcc gtgggggtcg gtctggctgg atcgcgaatc tgtgctgttt ggcgattcaa
    13861 cattggcact tcgtcagtgg atgcgcgaga aaggcattca gtttgaaatg aagcaaaacg
    13921 aaccggaaga tcattttggt tcactgctgt tgatggctgc gtggttggcg gagaatggtc
    13981 gccagacaga atgcgaagaa ttgctggcat ggcacctttt tccgtggtca acacgttttc
    14041 ttgatgtttt tatcgaaaaa gcagaacacc ctttctaccg tgcactgggt gaactggctc
    14101 gcttaacgct ggcgcaatgg caatcacaac tgttgattcc tgtcgcggtt aaaccgctgt
    14161 ttcgatagga tcaccgtaat attgccggat ggtgacgatt acgcgcaatc cggcaataat
    14221 aggttacagt gtcacgtttt tttatctctt aaagcacgca ctgcttttgc ggctggcctc
    14281 ttttgtcgca aaatagtcgc ccgtgtttca ttgcccattt ctgctcatgc atcatctaca
    14341 catctatccg gatctgcgca ctatgttccg ccgtctgctt atcgcaacag tcgtcggtat
    14401 tctcgcggcc tttgccgttg ccgggtttcg tcatgcgatg ctgctactgg agtggttgtt
    14461 cctcaataat gactccggca gtctggtcaa tgcagcgaca aacctttccc cctggcgacg
    14521 gttgctaact ccggcgctcg gcggactggc ggcgggtttg ttgctgatgg gctggcagaa
    14581 atttacccaa caacgccctc atgcgccgac cgattacatg gaagcgttgc aaaccgatgg
    14641 acagttcgat tacgcagcaa gcctggttaa atcgcttgcc tcgctgctgg tagtaaccag
    14701 cggcagtgca attggtcgcg aaggtgcgat gattctttta gctgcccttg ccgcctcctg
    14761 ttttgcccaa cgttttacgc cacgccagga gtggaaatta tggatcgcct gtggggccgc
    14821 ggcgggaatg gctgcggcct atcgtgcccc gcttgctggc agtttattta tagccgaagt
    14881 gctgtttggc actatgatgt tggcctctct cggcccggtg attatttccg ccgtcgtggc
    14941 attgctggtt agcaatctga ttaatcatag cgacgcgtta ctctacaacg tacaactctc
    15001 agtgacggtt caggctcgtg actatgcgct gattatcagt acaggtgtgc tggcaggtct
    15061 gtgcggacca ctgttgttaa cgttaatgaa cgcctgtcat cgtggatttg tgagtctcaa
    15121 acttgcgccg ccctggcaac tggcactagg cgggttgatc gtgggtctgc tttccctgtt
    15181 cacacctgca gtgtggggca acggctatag caccgtacaa tcctttttaa ccgccccacc
    15241 actgttaatg atcattgccg ggatcttcct ttgtaaactg tgtgccgtgc tggcgagtag
    15301 cggttccggc gcacccggtg gggtctttac accgacgcta tttatcggtc ttgccattgg
    15361 catgttgtat ggtcgtagcc tgggattatg gttccctgat ggcgaagaaa ttacactttt
    15421 actcggattg accgggatgg cgacactgtt ggcggcgacc acgcacgcgc cgattatgtc
    15481 gacgttgatg atatgtgaaa tgaccgggga gtatcagcta ctccccggtt tattgattgc
    15541 ctgcgtaatt gcgtcggtaa tttcgcggac gttacaccgt gactctatct accgccagca
    15601 cactgcgcag catagctaag cggatgtatt gccccagttc acgctgttcg gcgcgcggca
    15661 gataaggcag ttcaccaatg agcggtgccg gaagtttttt acccagcaca tcaatgattt
    15721 ccgcataatg cgccagtcct gggttgattc ggttagccac ccagccaatg agcggcagcc
    15781 cgtcgttggc gatcgcctga gctgttagca gtgcatggtt aatgcaacct tcctgaatac
    15841 cgacaaccat caacaccggc agttgttcct gcactaccca ttcagagagt ggacgcaaat
    15901 cattcatcag actgcgccag ccgccagtcc cttctaccac gacatgatcg actttttcgg
    15961 tcaggtttgc caggccgttt gaaatgaggg tgtaattgat tgggcaactg tgcgccacgc
    16021 tactttcttc ttcgcttaac gcgataggat taactgcttc ataaggcagt tcgatggttg
    16081 aaacactctg caacaccagg gcatctttat tacgcagccc ttcgggtgtc tctttgctcc
    16141 ccttcgctac gggtttatat cccgcaaccg tttttccctg ggaggctaac gcttgtagca
    16201 atgcgcggga aaccaccgtt ttccctacag aagtgtctgt accggtaata aagaaacgct
    16261 tcagcatcac taactccacc gttatgcttc acaaatataa accaggaaaa taattaacct
    16321 tgaaagtcta agttatgctt tcctggccca aattgagata gcgcaaattt tggtagaaca
    16381 gttaaaaaat gttaaccctg caacagacga atcaacaaag aaccgttata catcgcgtct
    16441 tttaccagtg cagcccctgc catcgtgccc tggttagaaa actgagtact ctcaacgctg
    16501 atgtgctgac tatacgcagg aagggcctgc tgacggatgc tgtctgagat gaccgggaag
    16561 aggatatctg ccgctttact taacggtgag ccaatcagta ttttttgtgg gttaaataaa
    16621 ttcaccatga tggcaagaat gcgccctaca tgcgcgccca ccccggtaat gatgtctttt
    16681 gccagcagat cgccgcgcaa tgccgcctga cacaatgagt ccacggttaa cggttgtcca
    16741 tgtaacatcg agctcatgga ttgattaaga cgcagctgtg ccagctcaag aatactgtcc
    16801 acactggcga tggtttcgag gcagccgtga ttcccgcaat aacagcgttt cccatacggg
    16861 tcgacctgtg tgtggcctat ttccacgaga ctactgctgc ctgcgtgtag cagatgacca
    16921 tcggtaatga cgcccgcccc cacgttgtga tcgataacca cctgaatcac atcgcgcgcc
    16981 ccgcgtgagg caccaaacaa ggcctctgcc atcgtccatg cgctgatatc atgctgaata
    17041 taaaccggta cgccggtatg ctgctccagc gcctcgccga gcggcatctc ttttacatcc
    17101 tcgtagaacg gcatgcgatg tacaatacca ttttccgtat caataattcc cagcaaggtt
    17161 atggcaatcg aagttagacg ctcaagtttt ttctggtggc gaataaaaaa ctgatcgata
    17221 tgggaaatga tacgatccag caatggcgag tcatctttta acgccagttc ctgcgactct
    17281 tccaccacca gtttgctgct cagatcgcgc agagcaagga aaatctcccc gcgactaatg
    17341 cgcagagaaa gatagtgcca ggcttcagtt tcaaccacca gccccaccgc cggacggcca
    17401 cggttccccg cttctttgat ttccagctct tgcaccaggt gtgcttcgag catctcacgg
    17461 acaattttag tgatactggc aggagccagc tgcgccagac gggaaagatc gatacgcgag
    17521 actggaccaa gctgatcaat caggcgataa accgcgcccg cgttggtctg ctttatttga
    17581 tcaatgtgcc caggctggtt ttcagcaacc accgcatact ccctatattt tcgcgctccg
    17641 aaataatctg taggctatgg tgaagcactt caatacgtgt cgtcaaattt ttacttaggc
    17701 atgtgattaa cagcacattt ttcgggcttt ttcgctgaaa tttccctcac ctgagagcat
    17761 tcagcagatg aatacgaaag tttcgcgcag ctggactttg ttcatgatgt ttcggccaga
    17821 ccaaccacat ttcagaaacc gcatcctctt ctgcaatcgg cacccagcgc atttcgttga
    17881 gctgaacacg tttaaatgac gcaggcaaaa ttgaaacacc cagaccggcg gaaaccagac
    17941 cgatgatggt cattgcctcg cccacctcct gagtgatgac gggcgtcaaa tggtaacgtc
    18001 gcatcagccc gagaatatct tcatacagcc ctgtcccgac gtgcggatca aaaaagacga
    18061 agggttcttt cgccagttca gccagcgtta cattcgggtt atttgccagg ggatgatcgt
    18121 gcgggatcat cgccataagc ggttcatgga cgatgactgc gtgttcaagc gtctccggta
    18181 acgctgtgtt acgcagcaat cccatatcca gcgttccttc aatgagcgga gcgatttgct
    18241 cacgagtgtt catttcgcgg gtttgtaaat ggacatcggg ataatcacgg cgaaacagcg
    18301 ataacgtatc ggacaccgcc cgaataaaag gagcggacga agtaaaacca atgcgcaact
    18361 cccccgcttc cccctgatgc agtctttcag cgcgagcggc agcgtcatct accatagaca
    18421 ggatttgccg actatctgca agaaactgtt ttcctgctgc cgtcagcaat acactgcgat
    18481 tagttcgtgc cagcagtcgg gcaccaattt gttgctccag tgcctgaatc tgctgactta
    18541 gcggtggttg cgaaatattc aggcgggcag cggcgcgccc gaaatgcagc tcttccgcaa
    18601 tagcaacaaa gtaacgcaga tgacgaagtt caatattcat atttaaaaca tcttatttga
    18661 gattattaat atattagaca gaacaattcg attttcctaa cctatgtata agcctgatcc
    18721 acaggcatat ttagcaagga tttcaagtga gccgtactac aactgttgat ggcgctccgg
    18781 caagcgacac tgacaagcaa agcatttctc agccaaatca atttattaaa cgcggtacgc
    18841 cgcaatttat gcgcgtcacc ctggcgctgt tctctgccgg actggcaaca tttgcacttc
    18901 tctattgtgt gcagcctatc cttccggtgc tttcgcagga gtttggctta acgcccgcga
    18961 acagtagtat ttcactgtcc atttccacgg cgatgttggc tattggtttg ctgtttactg
    19021 gccccctatc cgatgccatt ggtcgcaaac cagtgatggt cacggcgcta ctgttggcct
    19081 ccatttgtac gttactttcg acaatgatga ccagctggca cggcattttg attatgcgcg
    19141 ccttgattgg gctttcgtta agtggcgtgg cagctgttgg catgacttat cttagcgagg
    19201 aaatccaccc cagtttcgtg gccttttcga tggggttgta tatcagcggc aactcaattg
    19261 gcggcatgag cggacgctta attagtggtg tcttcacgga ctttttcaac tggcgaattg
    19321 ctctggcggc aatcggttgt ttcgcgctgg cctcggcgtt gatgttctgg aaaatccttc
    19381 cggaatcacg ccattttcgc ccgacttcgc tgcgccctaa gacgttattt atcaactttc
    19441 gtctgcactg gcgtgaccgg ggattaccgt tattgttcgc agaaggcttt ttgctgatgg
    19501 ggtcgttcgt cacgctgttt aattacatcg gctatcggtt gatgctttcc ccctggcatg
    19561 tcagtcaggc cgtggttggc ttattatcgc tggcttattt gaccggtaca tggagctcac
    19621 ccaaagccgg aaccatgacc acccgctacg ggcgcggtcc ggtgatgttg ttttcgacgg
    19681 gggttatgct gtttggttta ctgatgacct tattcagctc gctgtggctg atctttgccg
    19741 gaatgttact cttctcagca ggattcttcg ctgcccactc agttgccagc agctggattg
    19801 gcccccgcgc aaaacgcgct aaaggccagg cctcctcgct gtatctgttc agttactatc
    19861 tggggtcgag tattgccggg acgctgggtg gtgttttctg gcataactat ggctggaacg
    19921 gcgtcggcgc atttattgct ctgatgctgg tcattgctct gctggtcggg acgcgtttgc
    19981 atcgtcgtct gcacgcctga aaaataagcc cggactgcgg taactacccg tccggactta
    20041 ttgcttgctc aaaccaacgt taatagccat cctaaaatag acgaagcgcc agccaattcc
    20101 cgcagcgcgt ctagcgtcat caggattata agtacccaaa taaacggatt cattttgctg
    20161 tgtgtcattt attactgatg cgcagttatt ctaccgcttt gtaagtagta aaatagttaa
    20221 cccgatcaag actactatta ttggtagcta aatttccctt aagtcacaat acgttattat
    20281 caacgctgta atttattcag cgtttgtaca tatcgttaca cgctgaaacc aaccactcac
    20341 ggaagtctgc cattcccagg gatatagtta tttcaacggc cccgcagtgg ggttaaatga
    20401 aaaaacaaat tgagggtatg acaatgaaaa aagtattagc tctggttgtt gccgctgcta
    20461 tgggtctgtc ttctgccgcc tttgctgcag agactgcgaa cacacctgct ccgactgcga
    20521 cgaccaccaa agcagcgccg gcgaaaacta cacatcataa aaaacagcat aaagcagcac
    20581 ctgcccagaa agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag
    20641 cccctgaaca aaaagcgcag gcagcgaaga aacacgccgg gaaacacagc catcagcaac
    20701 cggcaaaacc tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc
    20761 ctcgcgcctg aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg
    20821 tagtatggtg ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact
    20881 gattgccgcc cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat
    20941 cccgtcccgt ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga
    21001 gtgtgatatg cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt
    21061 tgtgtttgca gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt
    21121 ttttggtcat gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt
    21181 tgggcaactg gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct
    21241 ggcattaacg gcaggacact gtttattgac gccgcctaag ggtaaagcgg ataaggcggt
    21301 agcgctgcgt tttgtgtcaa ataaaggtct ttggcgctat gacatccacg acatagaagg
    21361 ccgcgttgat ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgttcc
    21421 tcccgcagct gcaccatggg acttcggctt gattgtgctg cgtaatcccc cttctggcat
    21481 tacaccgttg ccgttatttg agggagataa agccgcgctt actgccgcat taaaatcggc
    21541 tggtcgtaaa gtgactcagg cgggatatcc tgaagatcat ctcgatacgt tatacagtca
    21601 tcaaaactgt gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac
    21661 cttgccgggt gacagcggtt cgcctctgat gttgcatacc gatgacggct ggcaattaat
    21721 tggggtgcaa agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat
    21781 ttctgttacc ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc
    21841 tgcccgacag tgcgggcagc gtgttcatca ggcaagtttc accatgatca ttccagccaa
    21901 cagcaagacc aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc
    21961 tgcggctaac gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc
    22021 gatccctttt acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag
    22081 caagccaaat attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat
    22141 ttccagcacg attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt
    22201 tcacctccag ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg
    22261 cgaccagggt ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacataa
    22321 cgctaaacaa ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta
    22381 aggcgatttt tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca
    22441 ttaaaataaa gccgccattt ccctcactga cgctcgccca tttcatggac agcgtaccgg
    22501 taatttctgt agcaatagcc agacctaata aaatccaata aatatacatt gtccttctcc
    22561 tgcaagagaa ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc
    22621 cccgcgcata gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc
    22681 tcacctacga gcaagatagt ggatgaggta cgaagatggg taaatgtaga aaataacgtc
    22741 ctgaacaaat tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat
    22801 gataattgtc ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca
    22861 aaatattttc acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa
    22921 gacattatta ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac
    22981 gctcaatggc ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat
    23041 ccgttttgcc gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttatata
    23101 taacttattt cacttaacgc attgattcaa ttaaataatt tacacaacaa aaagtgccaa
    23161 aaatcgcaaa atgactacac cagtgactac accgttcggt gcactgtatg aaacaacgtg
    23221 gaacaaatag acacaagaaa tataaaggcg ggtcatcttt ccagggggaa gcgcaccaat
    23281 tcatgagggg cgttaatgtc gatatgggga tccccaccta ccggaatttt tgccaggtta
    23341 aatgttaatc aggctggtgg gttttacata cccttgatca cggtaatgat gattcaggtg
    23401 cgtacctttt ccccagtggg gtaaaagctc caaccgtaac gatgttcgtt gtagttgcct
    23461 tgatggtctg cgtggtcatt atgaccattc agccaatgac cttaatcaga atttttcgct
    23521 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatggat
    23581 ggttaaaccg tctttatctc gatatccgta cgtattacgt acgcagtcgt tccggcttct
    23641 tccagtggta cgtaattttt gctttttccc tgtacgtctg cacgcggcga cggtaggcca
    23701 gcatttcaag aacgcggatc cgtatgttgc gcatatccac atcattaagc tggataccat
    23761 cacggcgcat cacctccgcg acaacacgcg cataattttc ggcggtcacg ctgtccggct
    23821 gcgtggcctg ttcgtcatgc tgctggctga ttccagagac gcggcggatt aatcgcagta
    23881 tttcggcttc tgtcatgctg ctgacctcat tacaccccgc taaattcttc cagtttctgg
    23941 cggtggctgt cgcttatatc aaaagcaaaa tcctcatgct ctgcctggaa tgtaccaaac
    24001 gccatcagtg ccgccacgct cgggtctatc ttgttcggtg attttttctt gttcggcttg
    24061 atattggcgt tcgcgtcact ctgcatcacg acgttactca tcgaccagga caacaccgga
    24121 tcaccacgat gcacgataac cctgcggtta acaaaaactt cgaacgattt cgccgccgga
    24181 ctgaatcgaa ggtaggtttg cgggaacggc tccacctcaa agcctgcccc ctgtaattgc
    24241 gtcctgagat gcgttgcgtt ccacgtatcg aagcccacca gcctgatatt aaatttttcc
    24301 gcgtcctgca tgatgtcatc acgtatccgg tcgtaatcaa tgcagtcccc tggcgttgtg
    24361 cgtatccatc ccgcccgtgc ccactggcga tagatagcgc gatttttatt ggctgggttc
    24421 tgtaactgga actccggcag ataatgacgg gaaaccagca tgatattttt accgaccgga
    24481 aaggcatagc acacgctgga aatgtcgctt gtggatgata aatccagccc cgcgtaacat
    24541 tcctgaccgt gtaaatcttc ctccgtgaac tttccggcgc actcagccca tgctccgtta
    24601 cccatccacg gggtagcccc ctgacaccag atattaaatc gctttgtcat catctccacc
    24661 cattgcgacg gtatgccccg cgctttctgg atggttgagg ccagtttttc acgatccacg
    24721 gaaacatcaa tattagggtt cgcctttatc cacatctccg gatcatcaac ctcgttttcg
    24781 tcgtccagtt cgtaaatcag tacaaaaatt gaatcgttga cctcttcgcc gtccaggatc
    24841 tggcagcaat aatcatagtg ctgtttacag gctgaaacga cgttgctgcc tgatgtggta
    24901 atggcaaata acagcccttc gggacgtgcc cccatcccca gttcaagcgc agaataaacg
    24961 ccgttatcgg ggtgtaagtg gtattcgtcc acaatggcaa ggctggggtt agtaccctcg
    25021 atggttgccg cttttgctgc cagcggcttt aacaggctgt tgcttttcgg gtgcatcacc
    25081 ttgtgggcct gaatattcac tctcttgcgt aacggtcggg ataaaaggca catctgacgc
    25141 gcatcatcaa aaacgatccg cgcctggtcg cggctcaccg ctgcggtgta aatatcctgc
    25201 tgcccgttct ccataatcag aaaccagtta gccagaatcg cggctgtcgt ggatttcgca
    25261 tttttgcgcg gtacttcgat aaaggcgctc gtgtatttgc gccttccggt ggccttaacc
    25321 ttaaagccga ggatgcacgc aaaggcaaac tgctgccacg gttccagctc aatgggtctt
    25381 ccacgcatcg gcccttttac gtgcgggcac accctggaaa agacaataaa ccgctcaaca
    25441 acctcacgat cgaacatgta aagggagttt tcaaggtccg aaaagtatcg tttaacggcc
    25501 tgtttcagcc gtttacaggc cggaatcttg cccgttttta cgtcttctgc gtacttattc
    25561 caggcggtca agctcgtcct cttcttctgt ttccggtgga tttttacggc ggcttatcgg
    25621 gtcaaaaccg agcaaagagg ccatttttat catcactctt tccgcgtcgg atttcgcgct
    25681 taatgcgggg tttctgctct ctccgccctg actgttaaca atgctgaacc cgcgcgccgc
    25741 aaggtctgcg acggctttcc ggtatatcga gtaattgacg caatacagtt ccagattact
    25801 ccagtcggca ggggtcaggt ctccccgttc cgcaagctgc cgcgattttt cccgccactg
    25861 cttcacggcg atatcatcca ggtaggcggg ggctttcggt ggtcttgcca tgcttatttt
    25921 ttcgccagat tatttttcaa aaaattaccg tgcataaaaa tttggggagg cgttcggttc
    25981 tgggcggggt cgggttcgtc ctgaaaactc cccccacccc gtccggctgc ctcatcagcg
    26041 attgcggaaa cattccataa cctcgcggtc acggtcgttt aatcgcgtcg ctgtgatgcg
    26101 ttctgcgcgt cctgatgctt tatctttatg ccctgcttcc tgtgccttcc atgcgtcacg
    26161 ctgccttata agtccacgga taaggcggtt ttgttcccgc tcattcatca gcgccataca
    26221 tccagttatt gcggttagcg ccccgttctt cctcctcacg gaatccaccc gcagcacgct
    26281 tgttttttgt ggacggatca agccatttcg ttttctggtt atgacacgcc tggcacaatg
    26341 gctgatgatt ccactcaggc cagaagagaa catcattacc gccatcgatt gggataatgt
    26401 gatccaccac cacggcgggc gtgtatattc ctttttccag gcaatgcgca cataacgggt
    26461 ttttactcag atacatggcg cggtatttct cccactgcct cgtataccct cgctccctgc
    26521 tgctgcctct ccggctgtcc tgctgctggc gtgcgctgcg tctgtgctcc tcacacttgc
    26581 cggacttcac gcgcctgttg cagcctggtt ctgtacatcg tcttaatggt tgccacggca
    26641 tcagtacacc cccacatcac ggtaagccgt ccagagtgcg ccaatcgtca tgggtacgtg
    26701 tgtttttgcg ttatccgcga caatctggcg attttcatac aggtgagcga taaacatcat
    26761 gcagccaatc tttatggctg gcgtgaactc cagcccgtca tcaaagcgcc tgcctatgtg
    26821 tatctggcac gcctcaagcg atgcggcaat gtatccgctg attaactggt cttcatcgtc
    26881 gccatcgatg cggcaatgga gtttcacttc ttccagggtg ataagttctg tcatttttcc
    26941 gcgccctcac tacaaagaat ttcaaggcgt gtcctggcgg catctggcag cggttgcccg
    27001 atgatattca gtacacgccc cgccagcggc cccgtattga cctttatccg actggtggcg
    27061 ttgatgtcct tccggtagcg tatccatatc cttacagttc cagtcgccag ctctgcacct
    27121 gatgaaatgg cctccttgct gctgatcatg ttcacgcttg cccagagtgt gtgaccgtcc
    27181 acccacgttt cgagtatttc gcccgtcatg gctctggtct gtttcagggt ctgaatcgtt
    27241 accctgtcac gcagtcgccc tatgttcatt ccggtttttc tccctcgctg atttttactt
    27301 cctgtttcca tgcctggctg aactcatcac caccatcacg cggtgacagt ccttcacgtt
    27361 cgcgggcttc gttcggacac ataacgccgg atttgatgcc gcgctcatag gtggcaaagc
    27421 gttcgccagg tgtggcccgt aacaggtccg cgctgtcaaa ctccacctga taccggatac
    27481 caggtacagg cgaggccacc agcagggcgt tttttatctg ctgctcaaag ttcgccagcc
    27541 acgggcgcat cgtcatggtg agaaatgcgc ggcttgcctc gctgaaattg ctgtaggtgc
    27601 tgttgctgta ttcctgcaaa aaaatcggcg agacgttgaa cattcgggcg atgtcttcaa
    27661 tggtgaagcg acgggaggcc agccattcag catcctgatt actcatgccc agctgctgat
    27721 agctcatacc cccttcaagg atgggcgttt ttccggcgtt tctggcccct ttgtagcgtt
    27781 ccagtgcggc taatgcctgt ttgcccttca cgccgtccag ccattcgcct gacgtgataa
    27841 cccctgccgc catcatgcca tcacgcatca cgctcgcgcc gtggcgttgt tgggccagcc
    27901 caagccccag cgattcgcgg caaatggtta caggtgagcg ccccataaaa ccgtcatccg
    27961 tggagtaacg aaggtggaga acctcccacg gtaaatagtt gcgggtgttt ccggtgtaag
    28021 cgtcagtgat gcagtagcgc cagttgtgtt ctcctgtctg ctccacgttc accgactgcg
    28081 gcgggtaagg atgtaaagcc gccggaaaac catcacgccc ccactgaatc accgcgtaag
    28141 cattaccgtt taacaggcag tggcggatca tcattcgctt aaactggtag ggagtctgcc
    28201 acgcgttcgg gcgctcgttg aggatgtgat cgaccggatg agaatcaagc cactcgcggg
    28261 cctccttccc cttctcattg cgtaccaggt acaggtagca tggcatagta gccaccgcct
    28321 cagagatgac cgtgacggcg ttcatgacgg cggggagtga ttccgctgtc cccgatgata
    28381 cgtactcacc cgcccccgtg ttcgatgtgc cagccagcgc cataaattca tcaagcgtca
    28441 tgctgcgctg ctcttttttt cttctgaaag gccacatatc acacccccgc taaatccgcc
    28501 caccagcggc gattatccgc acgcggcatt ttttcggggt gctgctcata cagggaacgg
    28561 cgggccagct ccacgccgga atcggggtaa gccggtacgg atgtaacggt aatttcgtac
    28621 agttccgcca ccagcacggt gcgcacgcat ggatctgttg tggtatccca tacatcctta
    28681 cgggaacgaa agccaaagct catgccggat atatcaccac gtttaaccag ttcgataacg
    28741 tcacgccctg tgctggtatc cggtggggta agttcaaagc gtaaccctgt gtcgtcctct
    28801 tccagtttca gcgtgccaga acgggtgcgc cccagtaaca tgctgtggtc atgctcatac
    28861 aggccgcgaa cgtcattacc cgccgcaagc cactcagtaa acgccccccg ctggaatttt
    28921 tcgtaaaact ccccccataa cagttccgaa aggttatccc agcgaacaac gtaacccgtc
    28981 agcgtgttgc tggcgctggt ggtgatttcc gatgaccgga tttccatact cttcataatt
    29041 tttttcaccc ataaaacact aaaggggctt tttagcccct tctgtatgct gttaatcgtc
    29101 gtcctgtggc agttccagaa tcttgatcgc gttcgaatcc accacgccac cgcctaaata
    29161 tttctgcgtg aaaattttga tgaatcctgg ttctgtcagg ttgtccggtc tggtgcgaac
    29221 acccgtttcg tgatcaacaa tgtagtaacc gcgtttgaag tcacccaggg caataacgtt
    29281 atccggcata aactccagat attcgaccgg aaggcccagc aacgtatcag gatcaccagc
    29341 ctgtaaacgg tcgcgccaga tgtaatcacc gttcgcgttc ttcaccttct gaagttttgc
    29401 cgccgtcgtg gagttaacca cccagaccgc gtttttgcgg tattttttac gtaatgcaaa
    29461 tttcaggtcg atcagcgggt ccgcagatgt ccacgcaaga gactcagaag gcttaattac
    29521 ctgcaacgta ccaaaatcac gttctttgtc gttcttctct gcacggggta cggataaaaa
    29581 gccttttgct tttttgtcac cgtcgcccac aaccagatcg ctttcttcgg tttccgtgaa
    29641 ggtgtcgcca atctcacccg tcagccatga aaggatgtcc acatcggaaa aatccacgat
    29701 ttcctgtgtg gtgcgcgggt acgcatagac cggatacagc ttaatgctca cctcgttaat
    29761 ctgcggggtg ctggtctgtt cgcgtgcctt accctcttca ccgtggttaa cggtcgcacc
    29821 gccagcggaa acaagctgct taaactcgtt gctgctgatt ttcttcacgg tacagatgcg
    29881 gcgcatggtg gattcatccg ccagcattcg catgatttcg gtgttcagct cggggataac
    29941 ggtataacca ccatcagcgg gaacgcctgt acttaatgcg cgggtttcac ccgtcagaat
    30001 gtagtttcgt agttctgcgg ggtcagtggt ctggctgctt ttacctggct tgctgcgctc
    30061 ttcgtctgca atggcttcaa ggcgggaaat gtctttatcg agggattcag ctttagcgcg
    30121 taattcgtca aattttgcgc cctctgcatc gttaaggctg cggttttctt tttccgcgtt
    30181 ctccagcatg tcgcgcatct gatttttaat ggcggttttc tgctggcgta attcgattat
    30241 tctcggcata aaaaaagtcc tggggttaag taaggaactc caggacgcgg caaaaactca
    30301 accgtttttc ataaggaaat cagcaatcgc accgatcgtt tttccgcctg gtaatgaata
    30361 ttggtgagca cattaacagg cgggaaagtg gccccagcgt cctggcacca ctggcgagaa
    30421 taatcatgat tcagggcggg taaaatatgc cgatccggtc aatgaacaac gtggaacgac
    30481 cacgaacaaa taatttacaa aaaatgacaa aaagccggat tgctccggct gtatgggtgc
    30541 tcagtccctg atccctttca gaaattctat caatgcatct atctgttcag gatttactgc
    30601 cagcatttca ccggatagcg cacatctgac aaatccatgc cgatcctttt cgattagcgc
    30661 gcccgtttcc aggaatgcgc ggtaatcatt gatgttcatc gtctccatgt tgtcagcatg
    30721 gtatttatca cgctgtttta atatctcatc aaatttcatc ggcattgttt tttcctctgt
    30781 tgtgtctgtt tgtttcagat agtaactatg ccggaccgtg acgaaaagcc ggtaatgcgc
    30841 cataccgttt caactggcgc aaaaaaagcc ggatttctcc ggctgtgtga ttagctgtcc
    30901 tggtaattgc gccgtatttc atcaccagca ccatctatac ccatttcggc acaagtgcga
    30961 ttaactgcat ttctcaggtc tccgaaatta tcaggcggct ccggtggcct ctgtgccttc
    31021 ctggaacatt ccagccgtcg catcgtgatg tgatgccgtt ccttgtctgt ctccaccagc
    31081 atcatgactt caccccatcg cgccgccgcc ctccggtaaa atcctttcgc ttcgagttct
    31141 tccgctatgc ggtcatgtac catcgtcacc ccctcagaac ggaatatcat caccgtaagg
    31201 gtcatcgcct cccgctggtg gctgattacc ctgtgtgcct gtggttttgc gtctgctgcc
    31261 gcctggtcgc accgttttcg cactgattac gctgtcggca atgacctgat aaccctgacg
    31321 cgtctccccg ttctgtccgg tccactggct tatctgcatc gtgccggata cgctgattac
    31381 gttgcctttc tcatgtcctg ccagttggtc ggcctgtttg ccaaatgcca ggaccgacag
    31441 ccataacgta gcctgcccgt cctgcgcctg actgcatggc agtgataccg ccatacgcgc
    31501 cagcgtcatc ggtgtgccct tgctggtctg ttttacctgc gggtcgtcca ccagccgccc
    31561 gtaagctgca atttgtgctg tcatattgcc acctcagtga agcgatccgg cgttgttctt
    31621 tttcatctct tccatcattt tttcagtgag cattacgcac tgaaaaccca tgtttttgat
    31681 gtctacgggt ttcatgcctt tgtcagtgga tatcacaggc agaatatcct gatacacatc
    31741 gcggtcaggg taactaagca cacaaagcgc ccatgatgcc acgggtgagg atccttcgtc
    31801 tgtttcaacc caccagcctg gtgtagctgc gcttatttgc agaatcgtga tccgtccgtt
    31861 gtgcttgtaa aatttttcac caaattttgg ttcgctcatg attccacctc tccggtttta
    31921 acgttgatgg ttgttacctg ttccgcttcg gcaatctccc gttctgtcag cgtggcaaag
    31981 tttgcagctg ccgt
//
LOCUS       DAZFXT010000049        31673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.049, whole genome shotgun sequence.
ACCESSION   DAZFXT010000049 DAZFXT010000000
VERSION     DAZFXT010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.049"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(72..1736)
                     /gene="glnS"
                     /locus_tag="ACHKW0_002399"
     CDS             complement(72..1736)
                     /gene="glnS"
                     /locus_tag="ACHKW0_002399"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HFO7475225.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1940..3886)
                     /gene="nagE"
                     /locus_tag="ACHKW0_002400"
     CDS             complement(1940..3886)
                     /gene="nagE"
                     /locus_tag="ACHKW0_002400"
                     /EC_number="2.7.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_function: GO:0015572 - N-acetylglucosamine
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HFO7475226.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDY
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4219..5019
                     /gene="nagB"
                     /locus_tag="ACHKW0_002401"
     CDS             4219..5019
                     /gene="nagB"
                     /locus_tag="ACHKW0_002401"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7475227.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKICSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5079..6227
                     /gene="nagA"
                     /locus_tag="ACHKW0_002402"
     CDS             5079..6227
                     /gene="nagA"
                     /locus_tag="ACHKW0_002402"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7475228.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6236..7456
                     /gene="nagC"
                     /locus_tag="ACHKW0_002403"
                     /gene_synonym="nagR"
     CDS             6236..7456
                     /gene="nagC"
                     /locus_tag="ACHKW0_002403"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HFO7475229.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7504..8256
                     /gene="nagD"
                     /locus_tag="ACHKW0_002404"
     CDS             7504..8256
                     /gene="nagD"
                     /locus_tag="ACHKW0_002404"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HFO7475230.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8513..10177
                     /gene="asnB"
                     /locus_tag="ACHKW0_002405"
     CDS             8513..10177
                     /gene="asnB"
                     /locus_tag="ACHKW0_002405"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HFO7475231.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKALVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10558..10634
                     /locus_tag="ACHKW0_002406"
     tRNA            10558..10634
                     /locus_tag="ACHKW0_002406"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10592..10594,aa:Met,seq:cat)
     gene            10643..10727
                     /locus_tag="ACHKW0_002407"
     tRNA            10643..10727
                     /locus_tag="ACHKW0_002407"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10677..10679,aa:Leu,seq:tag)
     gene            10751..10825
                     /locus_tag="ACHKW0_002408"
     tRNA            10751..10825
                     /locus_tag="ACHKW0_002408"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10783..10785,aa:Gln,seq:ttg)
     gene            10841..10917
                     /locus_tag="ACHKW0_002409"
     tRNA            10841..10917
                     /locus_tag="ACHKW0_002409"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10875..10877,aa:Met,seq:cat)
     gene            10966..11040
                     /locus_tag="ACHKW0_002410"
     tRNA            10966..11040
                     /locus_tag="ACHKW0_002410"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10998..11000,aa:Gln,seq:ctg)
     gene            11078..11152
                     /locus_tag="ACHKW0_002411"
     tRNA            11078..11152
                     /locus_tag="ACHKW0_002411"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11110..11112,aa:Gln,seq:ctg)
     gene            complement(11306..12481)
                     /gene="ubiF"
                     /locus_tag="ACHKW0_002412"
     CDS             complement(11306..12481)
                     /gene="ubiF"
                     /locus_tag="ACHKW0_002412"
                     /EC_number="1.14.99.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-demethoxyubiquinol 3-hydroxylase"
                     /protein_id="HFO7475232.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            12627..14051
                     /gene="miaB"
                     /locus_tag="ACHKW0_002413"
     CDS             12627..14051
                     /gene="miaB"
                     /locus_tag="ACHKW0_002413"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HFO7475233.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            14204..15244
                     /locus_tag="ACHKW0_002414"
     CDS             14204..15244
                     /locus_tag="ACHKW0_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HFO7475234.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            15241..15708
                     /gene="ybeY"
                     /locus_tag="ACHKW0_002415"
     CDS             15241..15708
                     /gene="ybeY"
                     /locus_tag="ACHKW0_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HFO7475235.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            15798..16676
                     /gene="corC"
                     /locus_tag="ACHKW0_002416"
     CDS             15798..16676
                     /gene="corC"
                     /locus_tag="ACHKW0_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HFO7475236.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            16701..18239
                     /gene="lnt"
                     /locus_tag="ACHKW0_002417"
     CDS             16701..18239
                     /gene="lnt"
                     /locus_tag="ACHKW0_002417"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HFO7475237.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYKSADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(18317..20194)
                     /locus_tag="ACHKW0_002418"
     CDS             complement(18317..20194)
                     /locus_tag="ACHKW0_002418"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HFO7475238.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNTLYAAIEDTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVAYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGEGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISIGDQQTSIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            20649..21557
                     /gene="gltI"
                     /locus_tag="ACHKW0_002419"
     CDS             20649..21557
                     /gene="gltI"
                     /locus_tag="ACHKW0_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HFO7475239.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPVAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            21727..22467
                     /gene="gltJ"
                     /locus_tag="ACHKW0_002420"
     CDS             21727..22467
                     /gene="gltJ"
                     /locus_tag="ACHKW0_002420"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HFO7475240.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            22467..23141
                     /gene="gltK"
                     /locus_tag="ACHKW0_002421"
     CDS             22467..23141
                     /gene="gltK"
                     /locus_tag="ACHKW0_002421"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HFO7475241.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            23141..23866
                     /gene="gltL"
                     /locus_tag="ACHKW0_002422"
     CDS             23141..23866
                     /gene="gltL"
                     /locus_tag="ACHKW0_002422"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP-binding
                     protein GltL"
                     /protein_id="HFO7475242.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            23984..24919
                     /gene="rihA"
                     /locus_tag="ACHKW0_002423"
     CDS             23984..24919
                     /gene="rihA"
                     /locus_tag="ACHKW0_002423"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050263 - ribosylpyrimidine nucleosidase
                     activity [Evidence IEA];
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HFO7475243.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPAFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            25003..26673
                     /gene="hscC"
                     /locus_tag="ACHKW0_002424"
     CDS             25003..26673
                     /gene="hscC"
                     /locus_tag="ACHKW0_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HFO7475244.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(26733..28184)
                     /gene="djlC"
                     /locus_tag="ACHKW0_002425"
     CDS             complement(26733..28184)
                     /gene="djlC"
                     /locus_tag="ACHKW0_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HFO7475245.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQMEEPESEDARYYLCAQRLYCGEGESLLADLCA
                     YRESYPSTQADNLLLQWSKSHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(28181..28888)
                     /locus_tag="ACHKW0_002426"
     CDS             complement(28181..28888)
                     /locus_tag="ACHKW0_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7475246.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEDSLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            28989..29543
                     /locus_tag="ACHKW0_002427"
     CDS             28989..29543
                     /locus_tag="ACHKW0_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7475247.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(29553..30980)
                     /locus_tag="ACHKW0_002428"
     CDS             complement(29553..30980)
                     /locus_tag="ACHKW0_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HFO7475248.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLGIPELLDYARSWREAESDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKPLLYIPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQANQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTEKQTVEPEPQPQPMPVDDGGPGC
                     LGIIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(30977..>31673)
                     /locus_tag="ACHKW0_002429"
     CDS             complement(30977..>31673)
                     /locus_tag="ACHKW0_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HFO7475249.1"
                     /translation="SQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWGID
                     ARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVASTA
                     ECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYFAG
                     YTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQPLAYY
                     SACPETLKDMSDL"
BASE COUNT         7904 a         8163 c         7914 g         7692 t
ORIGIN      
        1 cgtttgggat tgtttgctat ttcttgccgg atgcgacgta aatgcctcac ccggcctggt
       61 agaacttaaa attactcgcc tactttcgcc caggtatcac gcagcccaac ggtgcggtta
      121 aataccggtt tttccgctgt ggaatggcgg ctgtcgaggc agaagtaacc ttcacgctca
      181 aactggaatg ctttacccgc aaccgcatct ttcagcgacg gttcagcaaa gccctgtttg
      241 atcaccagcg attccgggtt aatcaccgac aggaaatcat ccgcagcacc agggttcggc
      301 acgctgaaca gacggtcgta cagacggatt tcaaccggca gcgcatgtgc cgcgctcacc
      361 cagtgaataa cacctttgac tttacgacca tctgccggat ctttgcttaa ggtgtcggcg
      421 tcataagtac agaagatggt ggtgatattg ccttcggcat ctttctccac gcgttcagcc
      481 ttaatgacgt aagcattacg cagacgcact tctttaccca gcaccagacg tttgtactgc
      541 ttgttagctt cttcgcggaa atcggcgcga tcgatccaaa tctcaccgct aaacggcacc
      601 tgacgactcc ccatttccgg tttgttcgga tggttcggca tggtgaccat ttcgccttcg
      661 ccctgatagt tttcgataac cagtttcacc ggatcgataa ccgccattgc gcgcggcgca
      721 ttttcgttga gatcttcacg gatgcaggat tccagcgatg ccatctcaat ggtgttgtcc
      781 tgcttggtca cgccgatgcg tttgcagaac tcacgaatag aagcagcagt gtaaccacga
      841 cgacgcagac cggaaatggt cggcatacgc gggtcatccc agccttcaac gtgcttgtcg
      901 gtcaccagca ggttcagctt acgcttggac atcacagtgt attccagatt caggcgcgag
      961 aattcgtact ggcgcgggtg aacaggaatg gtgatgttgt ccagcaccca gtcgtacagg
     1021 cgacggttgt cctgaaactc aagcgtacac agagagtgcg taataccttc cagggcatcg
     1081 ctgatgcagt gggtgaagtc gtacatcggg tagatgcacc acttgttgcc agtctggtgg
     1141 tgttcagcaa atttaatacg gtacagcacc ggatcgcgca tcacgataaa cggtgaagcc
     1201 atgtcgattt tcgcacgcag gcaggcttta ccttcttcaa aaccaccggc acgcattttt
     1261 tcgaacagcg ccaggttctc ttcaacgctg cggtcgcggt acgggctgtt tttacccggt
     1321 tgcgtcaggg tgccgcggta ttcgcggatc tgttccggcg tcagttcatc aacgtacgcc
     1381 aggcctttat tgatcagttc gatcgcatag gcgtggagct gatcaaaata atcggaggag
     1441 taacggacgt taccagacca gtgaaaacct aaccactcta cgtcgttttt gatcgactca
     1501 acatactcga tatcttcttt taccgggtta gtgtcgtcga aacgcaggtt gcactggcct
     1561 ttatagtcct gggcgatccc gaagttcagg cagatagatt tcgcatggcc aatatgcaga
     1621 tagccattcg gctccggcgg gaaacgggtg tgtactgtgg tgtgcttacc actggccaga
     1681 tcttcatcga tgatctgacg gataaagtta gtcgggcggg cttctgcctc actcatcgtg
     1741 gattcctcaa agcgtaaaca acgtataacg gcgtatgatc ttataagcgg gacaggctga
     1801 caactgttag ttttttaaaa ttgataatct aacaaggatt atggggatga ttgatgaaaa
     1861 aaaaaagcgg cagagaagat ctccaccgct tgtcattgtt ggatgcgacg ctcaagcgtc
     1921 gcatcaggca taaagcagat tactttttga tttcatacag cggtgtttga cccgccacaa
     1981 catggccctg agctttaatg atcaagccac tgaaatagtc gatattgctg caaaccaccg
     2041 ggctaatcat cgagcgggcg ttagcgttca ggtaatccag atccatttcc agaatcggtt
     2101 gccctgcgct tacctgcgca ccctcttcca ccagacgttt aaagccttta ccttccagcg
     2161 ctacggtgtc gatacccata tggacgacga tctccgcgcc tttttcggtt tccaggcaga
     2221 acgcgtggtt ggtgttgaag attttcacga ttgtccctgc ggctggtgat acgacgattt
     2281 tatctgtcgg tttcaccgcc acaccgtcac ccaccgcttt gctggcgaat gcttcgtcag
     2341 gaacctgatc cagtgccacg acatcaccgg taatcggcga taccagctcc gcgatagata
     2401 ccgcgtttgg tacagcctgc ggttttgcta caggcgcggc agttgccgga gttgcttcag
     2461 ctgacgcagc ggctaccgga ccacgggcaa cgactttctt catcgcatcg ccgatggatt
     2521 ctgctttcgc gccaacaatc acctgaatag tctgtttgtt cagtttcact acaccagaag
     2581 cacccagacg tttacacatc gtatcgttga cgcgggcaga gtcagccacg gtcaggcgca
     2641 gacgggtgat acaggcgtca attgctttca ggttgtcagt gccgccaacc gcagcaatat
     2701 agttggtcgc cagttgattg aggccttctt cagtgttgct gttggcttct tcagtaacga
     2761 tctcgtcttc tttatcttca cgacccggcg ttttcaggtt gaacatgcgg ataaccaaac
     2821 tgaacaccac gaagtagata gcgaagaaga caacacccat aaccagcagc atccagacgt
     2881 tctggctggc ggccggcagg ttatacatca acgcgtagtc gatagccccc gcagagaaag
     2941 agaagccagc atggataccc agcagtgttg ccacaaacag gctgataccg gtcagcagtg
     3001 cgtgcaggag gtacagcagc ggtgcaagga acatgaacag gaattccagc ggctcagtca
     3061 caccggtcag gaacgcagta acagcaacag aaagcagcat cccgccaacc atcggacgac
     3121 gctctttcgg tgctgcgaag tacatcgcca gcgccgcacc cggcagaccg aacatcataa
     3181 tcgggaagaa gccggacatg aacatccccg cggtgccgtc accggcatag aagcggttga
     3241 tatcaccgtg gaaaaccgta cccgccgcgt tggtgaattc accaatctgg aaccaggcga
     3301 tggtgttcag cacctgatgc agaccggttg ggatcagcag acggttgatg aaaccaaaga
     3361 taccggaacc cagcgcgccc gcagaaacga tccactcgcc tcctgcatgg atagcgtgct
     3421 gtaccggcgg ccagacgtaa ccaaaaatgg ccgccagcac caggcagaag aagccggtgg
     3481 caatcggcac aaagcgtttg ccgccgaaga aactcaggaa gtccggcagt ttaatatcgg
     3541 accaacggtt ataggctgcg ccaccaacca gaccggtaat gatacccgcc agtacaccca
     3601 tgttaatttc tgggttgatg gtcaccatcg ctttggttaa cacaaagtaa cctaccgcac
     3661 ccgccagcgc cgccgcacct gcgctgtctt tcgaccagct ggatgccaca ccgatggcga
     3721 agattaatgc gaggttatca aaaatcgcac cgcccgcctg ggcaataaac gcaacgttaa
     3781 gtaaatctgg ctgaccgaat cgcagcaaca gtgccgccac cggcagcacc gcgataggga
     3841 gctgtaacgc cctaccgagt cgctggaaaa aacctaaaat attcatctta ttccccctac
     3901 gagaacccta tttggctcgt ttaaagccgt atttttattt tgctgcaaat tgtactgccg
     3961 atgttctgta atcagattgt tagatcatct gctacagagt gtgtgaaaat ttaattcgta
     4021 tcgcaaatta aacgcgtgtc ttttgtgagt tttgtcacca aatatcgtta tcatcactcc
     4081 cttttactgg ctaaagcaga aaacttattt tatcattcaa aaaatcaggt cggattgacg
     4141 cctgtctgcg caaatccagg ttacgcttaa agatgcctaa tccgccaacg gcttacattt
     4201 tacttattga ggtgaataat gagactgatc cccctgacta ccgctgaaca ggtcggcaaa
     4261 tgggctgctc gccatatcgt caatcgtatc aatgcgttca aaccgactgc cgatcgtccg
     4321 tttgttctgg gcctgccgac tggcggcacg ccgatgacca cctataaagc gttagtcgaa
     4381 atgcataaag caggccaggt cagctttaag cacgttgtca ccttcaacat ggacgaatat
     4441 gtcggtctgc cgaaagagca tccggaaagc tactacagct ttatgcaccg taatttcttc
     4501 gatcacgttg atattccagc agaaaacatc aaccttctca acggcaacgc cccggatatc
     4561 gacgccgaat gccgccagta tgaagaaaaa atctgttctt acggaaaaat tcatctgttt
     4621 atgggcggtg taggtaacga cggtcatatt gcatttaacg aaccggcgtc ttctctggct
     4681 tctcgtactc gtatcaaaac cttgactcat gacactcgcg tcgcgaactc tcgtttcttt
     4741 gataacgatg tcaatcaggt gccaaaatat gccctgactg tcggtgttgg tacattgctg
     4801 gatgccgaag aagtgatgat tctggtgctg ggtagccaga aagcactggc actgcaggct
     4861 gcagttgaag gttgcgtgaa ccacatgtgg accatcagct gtctgcaact gcatccaaaa
     4921 gcgatcatgg tgtgcgatga accttccacc atggagctga aagttaagac tttaagatat
     4981 ttcaatgaat tagaagcaga aaatatcaaa ggtctgtaat tgttatccct gccctggctc
     5041 cttgctcagg gcaatatttt ttaaaatcgg gggtcagaat gtatgcatta acccagggcc
     5101 ggatctttac cggccacgaa tttcttgatg accacgcggt tgttatcgct gatggcctga
     5161 ttaaaagcgt ctgtccggta gcggaactgc cgccagagat cgaacaacgt tcactgaacg
     5221 gggccattct ctcccccggt tttatcgatg tgcagttaaa cggctgcggc ggcgtacagt
     5281 ttaacgacac cgctgaagcg gtcagcgtgg aaacgctgga aatcatgcag aaagccaatg
     5341 agaaatcagg ctgtactaac tatctgccga cgcttatcac caccagcgat gagctgatga
     5401 aacagggcgt gcgcgttatg cgcgagtacc tggcaaaaca tccgaatcag gcgttaggtc
     5461 tgcatctgga aggtccgtgg ctgaatctgg taaaaaaagg cacccataat ccgaattttg
     5521 tgcgtaagcc tgatgccgcg ctggtcgatt tcctgtgtga aaacgccgac gtcattacca
     5581 aagtgaccct ggcaccggaa atggttcctg cggaagtcat cagcaaactg gcaaatgccg
     5641 ggattgtggt ttctgccggt cactccaacg cgacgttgaa agaagcaaaa gccggtttcc
     5701 gcgcggggat tacctttgcc acccatctgt acaacgcgat gccgtatatt accggtcgtg
     5761 aaccgggcct ggcgggcgcg atcctcgacg aagctgacat ttattgcggt attattgctg
     5821 atggcctgca tgttgattac gccaacattc gtaacgctaa acgtctgaaa ggcgacaaac
     5881 tgtgtctggt taccgacgcc accgcgccag caggtgccaa cattgaacag ttcatttttg
     5941 cgggtaaaac aatatactac cgtaacggac tttgtgtgga tgagaacggt acgttaagcg
     6001 gttcatcctt aaccatgatt gaaggcgtgc gtaatctggt cgaacattgc ggtatcgcac
     6061 tggatgaagt gctgcgtatg gcgacgctct atccggcgcg tgcgattggc gttgagaaac
     6121 gtctcggcac actcgccgca ggtaaagtag ccaacctgac cgcattcaca cctgatttta
     6181 aaatcaccaa gaccatcgtt aacggtaacg aggtcgtaac tcaataagag aaagtatgac
     6241 accaggcgga caagctcaga taggtaatgt tgatctcgta aaacagctta acagcgcggc
     6301 ggtttatcgc ctgattgacc agtacgggcc aatctcgcgg attcagattg ccgagcaaag
     6361 ccagcttgcc cccgccagcg taaccaaaat tacgcgtcag cttatcgaac gcgggctgat
     6421 caaagaagtt gatcagcagg cctccaccgg gggccgccgc gctatctcca tcgtcaccga
     6481 aacccgcaat ttccacgcaa tcggcgtacg gcttggtcgt cacgacgcca ccatcaccct
     6541 gtttgatctc agcagcaaag tgctggcaga agaacattac ccgctgccgg aacgtaccca
     6601 gcaaacgctg gaacatgccc tgttgaatgc cattgctcag tttattgata gctaccagcg
     6661 caaactgcgc gagctgatcg cgatttcggt gatcctgcca gggcttgttg acccggacag
     6721 cggcaaaatt cattacatgc cgcatattca ggtagaaaac tgggggctgg tagaagcact
     6781 ggaagaacgt tttaaagtga cctgtttcgt tggtcacgat atccgtagtc tggcgctggc
     6841 ggagcactac ttcggtgcaa gtcaggattg cgaagactcc attctggtgc gtgtccatcg
     6901 cggaaccggg gccgggatta tctctaacgg gcgcattttt attggccgca acggcaacgt
     6961 cggtgaaatt ggccatattc aggtcgaacc gctgggtgaa cgctgccact gcggcaactt
     7021 tggctgcctg gaaactatcg ctgccaacgc cgctattgaa caacgggtgt tgaatctgtt
     7081 aaagcagggc taccagagcc gcgtgccgct ggacgactgc accatcaaaa ctatctgcaa
     7141 agccgcgaac aaaggcgata gcctggcctc ggaagtgatt gagtatgtcg gtcgtcatct
     7201 gggcaaaacc atcgccattg ctatcaactt atttaatccg caaaaaattg ttattgccgg
     7261 tgaaatcacc gaagccgata aagtgctgct ccctgctatt gaaagctgca ttaataccca
     7321 ggcgctgaag gcgtttcgca ctaatctgcc ggtggtacgt tctgagctgg atcaccgctc
     7381 ggcaatcggc gcttttgcgc tggtaaaacg cgccatgctc aacggtattt tgctccagca
     7441 tttgctggaa aattaatgtg cttttatagt ggcgcttatt gttgtcaata ttctgggtag
     7501 tccatgacca ttaaaaatgt aatttgcgat atcgacggcg tgctgatgca cgataacgtc
     7561 gccgtaccgg gtgcagcgga atttttgcac gggattatgg ataaaggcct gccgctggtg
     7621 ttgctgacca actatccttc gcagaccggg caagatctgg cgaaccgctt tgccactgcc
     7681 ggtgtcgatg tgccggatag cgtgttttat acctctgcga tggcgactgc cgatttcctg
     7741 cgtcgccagg aaggcaagaa agcgtatgtg gtgggcgaag gcgcactgat tcatgaactg
     7801 tacaaagccg gtttcactat taccgatgtg aaccctgatt ttgtgattgt tggcgaaacg
     7861 cgttcctaca actgggacat gatgcataaa gcggcctatt tcgtcgctaa cggtgcacgc
     7921 tttatcgcca ccaacccgga cacccacggg cgcggttttt atcccgcttg tggcgcgttg
     7981 tgtgcaggga ttgagaaaat ctccgggcgc aaaccgttct atgttggtaa gcccagcccg
     8041 tggatcatcc gcgcggcatt aaacaaaatg caggcgcatt cggaagaaac ggtgattgtc
     8101 ggcgataact tgcgcaccga cattctggcg ggcttccagg cgggtctgga gacgattctg
     8161 gtgctttctg gtgtttcatc gctcgacgat atcgacagta tgccgttccg cccaagctgg
     8221 atttacccgt cggtcgctga aatcgacgtt atctgaatag caaccacgtc tcagggcgtg
     8281 gtttcttatt tttacccatc attaaaatca tcccattcaa taaaactcgc caaaaattat
     8341 cgatccatca ataaatacat caccacaccc tccccttttt cacaaatcag caatcaccat
     8401 tacgtttttt atttttgcac tgccaaatcg cttgcgcacg gtgctgcttt acggcatttt
     8461 tttaaacaag caaacacaac aagcaacaaa taccaggtta acggagaagg ttatgtgttc
     8521 aatttttggc gtattcgata tcaaaacaga cgcagttgag ctgcgtaaga aagcactcga
     8581 gctgtcacgc ctgatgcgtc atcgtggccc ggactggtcc ggtatttatg ccagcgataa
     8641 cgccattctc gcccacgaac gtctgtcaat tgttgacgtt aacgcggggg cgcaacctct
     8701 ctacaaccaa caaaaaactc atgtgctggc ggtaaacggt gaaatctaca accaccaggc
     8761 actgcgcgcc gaatatggcg atcgttatca gttccagacc ggatctgact gcgaagtgat
     8821 cctcgcgctg tatcaggaaa aagggccgga atttctcgac gacttgcagg gcatgtttgc
     8881 ctttgccctg tacgacagcg aaaaagatgc atatctgatt ggtcgcgacc atctggggat
     8941 catcccactg tatatgggct atgacgagca cggtcagctg tatgtggcct cagaaatgaa
     9001 agccctggtg ccagtttgcc gcacgattaa agagttcccg gcggggagct atttgtggag
     9061 tcaggacggc gaaatccgtt cttattatca tcgcgactgg ttcgactttg atgcagtgaa
     9121 agataacgtg accgacaaaa acgagctgcg tcaggcactg gaagattccg ttaaaagcca
     9181 tctgatgtct gacgttcctt acggtgtgct gctctcgggt ggtctggatt cctcaattat
     9241 ttccgcgatc accaagaaat acgccgcccg tcgcgtggaa gatcaggaac gctctgaagc
     9301 ctggtggccg caattacact cctttgctgt aggtctgccg ggttcaccgg atctgaaagc
     9361 agcccaggaa gtggcaaacc atctgggcac ggtgcatcac gaaattcact tcactgtaca
     9421 ggaaggtctg gatgccatcc gcgacgtgat ttaccacatc gaaacctatg atgtgacaac
     9481 tattcgcgct tcaacaccga tgtatttaat gtcgcgtaag atcaaggcaa tgggcattaa
     9541 aatggtgctg tccggtgaag gttctgatga agtgtttggc ggttatcttt acttccacaa
     9601 agcaccgaat gccaaagaac tgcatgaaga gacggtgcgt aaactgctgg ccctgcatat
     9661 gtatgactgc gcgcgcgcca acaaagcgat gtcagcctgg ggcgtggaag cacgcgttcc
     9721 gttcctcgac aaaaaattcc ttgatgtggc gatgcgcatt aacccgcagg ataaaatgtg
     9781 cggtaacggc aaaatggaaa aacacatcct gcgtgaatgt tttgagtcat acctgcccgc
     9841 aagcgtggcc tggcggcaga aagagcagtt ctccgatggc gtcggttaca gttggatcga
     9901 caccctgaaa gaagtggcgg cgcagcaggt ttctgatcag caactggaaa ctgcccgctt
     9961 ccgcttcccg tacaacacgc caacctcaaa agaagcgtat ctgtaccggg agatctttga
    10021 agaactgttc ccgcttccga gcgccgctga gtgcgtgcct ggcggtcctt ccgtcgcgtg
    10081 ttcttccgct aaagcgattg agtgggatga ggcgttcaag aaaatggacg atccgtctgg
    10141 tcgtgcggtt ggcgttcacc agtcggcgta taagtaagaa atgcgaaaca gccccggtga
    10201 aatccggggc tgtttcagtt attgaggtgc ttgttaattc tgatatgcag taaggaaatg
    10261 ttccgggttt gccacatcac tgtttgacaa ctcatcactg taggccagta tttcatagat
    10321 taatttccca ccactattgt tgaattccga atttcatcct gtacgacaaa agaatggctg
    10381 tagggtgggg aaaaaacaca aagtaaacaa taattgacga atatagcgcc acgctgttcg
    10441 caacctaacc aaacagtcac tttcgagcaa ttttccttga aaaagaggtt gacgctgcaa
    10501 ggctctatac gcataatgcg ccccgcaacg ccgataaggt atcgcgaaaa aaaagatggc
    10561 tacgtagctc agttggttag agcacatcac tcataatgat ggggtcacag gttcgaatcc
    10621 cgtcgtagcc accatctttt ttgcgggagt ggcgaaattg gtagacgcac cagatttagg
    10681 ttctggcgcc gcaaggtgtg cgagttcaag tctcgcctcc cgcaccattc accagaaagc
    10741 gttgtacgga tggggtatcg ccaagcggta aggcaccggt ttttgatacc ggcattccct
    10801 ggttcgaatc caggtacccc agccatcgaa gaaacaatct ggctacgtag ctcagttggt
    10861 tagagcacat cactcataat gatggggtca caggttcgaa tcccgtcgta gccaccaaat
    10921 tctgaatgtg tcgaatacgt tcggcaaatt caaaaaccaa tttgttgggg tatcgccaag
    10981 cggtaaggca ccggattctg attccggcat tccgaggttc gaatcctcgt accccagcca
    11041 atttattcaa gacgcttaca tcgtaagtgc gcccagttgg ggtatcgcca agcggtaagg
    11101 caccggattc tgattccggc attccgaggt tcgaatcctc gtaccccagc cacattaaaa
    11161 aagctcgctt cggcgagctt tttgcttttc tgcgttcatt caatgtcgaa tgcgatgttg
    11221 acacgtctta tccttcaatg tcggatgcga cgctgccgcg tcttatccga cctacggttg
    11281 gcacgcatcc ggcaatgttg taaggctaca accctaacgc atatttcagc gcctgacgtt
    11341 tcaacacgcc agcacgctct gccgccatta acccgagatt acgcacaaaa cgcagcggcg
    11401 gcagattatt actgaatccg gcataaaaca gatccatacc actttgcata atgaagttat
    11461 ccgccatgcg ccgcatctgg taacgcttga ggacaggata actggcccac gcttcaccgt
    11521 agctgcgggc attcaccaga acatcaatca gggcatcgac atcacgataa ccaagattca
    11581 ccccctgccc cgccagcgga tggatggtat gcgcggcatc gcccaccagc gcaagccccg
    11641 gctgcacata ctgcaacgca tgtcggcgcg tcagcggaaa cgcaccagcg gcaagcggtg
    11701 taacgtaacc cagacgcgac gggaaatgct tcgcgatttc cgcctggagt tgcgccatat
    11761 tcatattctg caactggcga atacgtaccg gagagtcata ccacaccagc gatgcccagt
    11821 tatcaaacaa cggcagaaac gcacgcggtc cgtccggggt aaattgctgc caggtgctgt
    11881 cgccgggatc gttctcacac tgtacgctaa tcaacataca cgactgcgca tactgccagg
    11941 catgaacgcc aattcccgcc atctgccgca cctgcgaatt tgcgccgtca gcaccaatca
    12001 ccagcttcgc gcgaatcact tcaccgccct tcagttccag ctcctgaaga tcattatctc
    12061 gatgtaatgc aatcagcgag cctggcacac gtaacgttac tttcggatgc gcttccagcg
    12121 cctgccacaa tgcctgttgc aggacggtgt tttccaccat atagccaagc agcggcagct
    12181 taagttcagc ggcgtcaaac atcacatgcg ccgtttccca ctcccacgtt tccagtctgc
    12241 ggtaaggatg gcaacgcata gcctgtactg catcccagac ccctaaccct ttaagcaatg
    12301 ataccgatgc ggcgctgatc gccgagatcc gcacgtccgg ttggctatca gcgacaaacg
    12361 gtgcaggttc tgcgtgctcg atcaccgtta ccgaaaatcc gtgctgtgcc agccccagcg
    12421 ccagtgcgcc gccgaccatt cctccgccga caatggcaat ttccgttggt tgatttgtca
    12481 tggtcaatca tcctgttagc aatatgcttt aagtttacag gatttttacc cccaagggct
    12541 gataacgctc atactggtca caacggcagc aaagcattac actatgcgcc ctgcattcct
    12601 ggctactatt tcgcaagagc aagtcgatga ccaaaaaact ccatattaaa acctggggct
    12661 gtcagatgaa cgagtacgat tcatcgaaga tggccgatct gctggatgcc acccacggct
    12721 atcaactgac cgacgtggcg gaagaagcgg atgtgctgct gctgaacacc tgctcaatcc
    12781 gcgagaaggc tcaggaaaaa gtcttccatc agttgggtcg ctggaaactg ttaaaagaga
    12841 agaatccaga cctgattatc ggcgtcggtg gctgcgtggc atcgcaagaa ggcgagcaca
    12901 ttcgccagcg cgcccactat gtcgatatta tttttgggcc gcaaacgctg caccgtctgc
    12961 cggagatgat caactccgtg cgcggcgacc gcagcccggt tgtagatatc agcttcccgg
    13021 aaatcgagaa gtttgaccgt ctgccggaac cgcgcgccga agggccgacc gcgtttgtct
    13081 ccatcatgga aggctgcaat aaatattgca cctactgcgt ggtgccttac acccgtggtg
    13141 aagaggtaag ccgtccgtcc gacgatattc tgtttgagat cgcccagctt gcggctcagg
    13201 gcgtgcgtga agtcaacctg ctcggtcaga atgtgaatgc ctggcgtggt gagaactacg
    13261 acggcaccac cggatcgttt gccgatctgt tgcgtctggt tgctgcgatc gacgggatcg
    13321 atcgtattcg ctttaccacc agccatccga tcgaattcac cgacgatatc atcgaagtgt
    13381 atcgcgacac gccggagctg gtgagcttcc tgcatctgcc ggtacagagc ggttccgatc
    13441 gcattctgaa cctgatgggc cgtactcata cggcactgga gtacaaagcg atcatccgta
    13501 aactgcgtgc ggcgcgtccg gatattcaga tcagttctga cttcattgtt ggcttccctg
    13561 gcgaaaccac cgaagacttc gagaaaacga tgaagctgat tgccgacgtc aatttcgaca
    13621 tgagctacag ctttatcttc tctgcacgtc caggaacgcc tgccgctgat atggttgatg
    13681 atgttccgga agaagagaag aagcagcgtc tgtatattct gcaagaacgt atcaatcagc
    13741 aagcgatggc atggagccgt cggatgctcg gcaccaccca gcgtattctg gtagaaggta
    13801 catcgcgtaa gagcatcatg gagctttccg ggcgtaccga aaataaccgt gtggtcaact
    13861 tcgagggcac accggatatg atcggtaaat tcgtcgatgt agaaattacc gacgtctacc
    13921 cgaactctct gcgcggtaaa gtggtgcgta ctgaagatga aatgggtctg cgcgtggcag
    13981 aaacacccga atcggtgatt gcccgtactc gcaaagaaaa tgaccttggc gtgggttatt
    14041 atcagccgta attctcaggc ccgccgttcc ggtgggcctt gttttttctc tctttatccc
    14101 catctttatc gcaatgcttg cgcctttttc ctgtggcgtg aataatttcc ttataggccg
    14161 gtgaatgttc agcttccggc ggcatacaga taagaggaac ggtttgaaca tagacactcg
    14221 cgaaatcacc ctggagccag cagacaatgc gcgtctgttg agcctgtgcg gcccgtttga
    14281 tgacaacatc aagcagctcg aacgccgtct cggcatcgag atcaatcgcc gcgacaacca
    14341 ctttaaactg accggccgtc cgatttgcgt caccgctgcg gcagacattc tgcgtagcct
    14401 gtatgtcgat acagccccga tgcgcggtca gattcaggat atcgaaccgg aacagatcca
    14461 ccttgcgatt aaagaagcgc gagtactgga gcaaagtgcg gagagcgtgc cggagtacgg
    14521 caaagcggtc aatatcaaaa ccaaacgcgg cgtaattaag ccgcgcacgc caaaccaggc
    14581 gcagtacatc gccaatattc tcgaccatga cattaccttc ggcgttggcc cggcgggtac
    14641 gggtaaaacc tacctggcag tggctgcggc agttgatgcc ctggagcgtc aggagattcg
    14701 ccgtattctg ctgactcgtc cggcggtcga agccggtgag aaactgggct tcctgcctgg
    14761 cgatttaagc cagaaagtag acccgtatct gcgcccgctg tacgacgcgc tgtttgaaat
    14821 gctgggcttt gagaaagttg agaaactgat tgagcgcaac gttattgaag tcgcgccgct
    14881 ggcctatatg cgtggtcgta cgctgaacga cgcgtttatc attctcgatg agagccagaa
    14941 caccaccatc gaacagatga agatgttcct gacccgtatc ggttttaact caaaagcggt
    15001 tatcaccggc gacgtcacac agatcgacct gccgcgtaat actaaatcag gcttacgtca
    15061 cgccatcgaa gtgctggccg atgtcgaaga gatcagcttt aacttcttcc acagcgaaga
    15121 cgtggttcgt cacccggtgg tggcgcgtat cgttaacgcc tatgaagcct gggaagaagc
    15181 cgaacaaaaa cgaaaagcgg cgctggcggc agaacgcaag cgcgaagaac aggaacaaaa
    15241 atgagtcagg tgatcctcga tttacaactg gcatgtgaag ataattctgg gttaccggaa
    15301 gagagccagt ttcaaacatg gctgaatgcg gtgatcccgc agtttcagga agaatcggaa
    15361 gtgactattc gcgtggtcga taccgccgaa agccacagtc tgaatctgac ctatcgcggt
    15421 aaggataagc cgaccaacgt gctctccttc ccgtttgaag tgccgcctgg catggaaatg
    15481 tcgctactgg gcgatctggt tatctgccgt caggtggttg agaaggaagc tcaggagcaa
    15541 ggcaaaccac tggaggcgca ctgggcgcat atggtggtgc acggcagtct gcatttgtta
    15601 ggttacgatc acatcgaaga tgacgaagca gaagaaatgg aagccctcga aacagagatt
    15661 atgcttgctc tgggctatga ggatccgtac attgccgaga aagaataatt tgccaggcat
    15721 ttgactggca gacgtccccg ccgttggtga ttaacgacgg cgttttgatt aactaacatg
    15781 agaacccata cgacgccatg agcgacgaca attcacacag tagtgacacg ataagcaaca
    15841 agaagggatt tttctccctg ttactcagcc aacttttcca cggtgaaccg aaaaaccgtg
    15901 acgaactgct ggcgctgatc cgtgattccg ggcagaacga ccttatcgac gaagatacgc
    15961 gcgatatgct cgaaggggtg atggacatcg cagaccaacg cgttcgcgac atcatgatcc
    16021 cccgctccca gatgattacc ctgaaacgca accagacgct ggacgaatgc cttgatgtca
    16081 tcatcgagtc cgcccactca cgtttcccgg tgattagcga agacaaagat cacattgaag
    16141 ggattctgat ggcgaaagac ttgctgccgt ttatgcgcag cgatgctgaa gccttcagca
    16201 tggacaaagt gttacgtcag gcggttgtcg ttcctgaaag taagcgcgta gaccggatgc
    16261 tgaaagagtt tcgctctcag cgttaccaca tggcgatcgt tattgacgaa ttcggtgggg
    16321 tttccggtct ggtgaccatt gaagacatcc tggaactaat tgttggtgag attgaagacg
    16381 agtatgacga agaagatgat atcgacttcc gtcagctgag tcgtcatacc tggaccgtgc
    16441 gcgcactggc ttccattgaa gacttcaacg aagcgttcgg cacccacttt agcgatgaag
    16501 aggttgacac tatcggtggt ctggtgatgc aggcatttgg gcatcttccg gcgcgtggcg
    16561 aaactatcga catcgacggt taccagttca aagtggcgat ggccgacagt cggcgtatta
    16621 ttcaggttca tgtcaaaatc ccggatgact caccccagcc gaagctggat gaataaaacc
    16681 gaaactggat agataactac atggcttttg cctcattaat tgaacgccag cgcattcgcc
    16741 tgctgctggc gttattattc ggtgcctgcg gaacgctggc cttctctcct tacgatgtct
    16801 ggcctgcggc gattatttcg ctgatggggc ttcaggcgtt gacctttaac cgccgtccac
    16861 tccagtctgc cgctattggc ttttgctggg gatttggcct ctttggcagc ggtattaact
    16921 gggtctatgt cagcatcgcg acctttggcg ggatgcctgg cccggttaac atcttcctgg
    16981 tggtgctgct ggcggcgtat ttgtcgctgt ataccggact gtttgctggc gtgctgtcgc
    17041 gtctgtggcc gaaaaccacc tggctgcgcg tggcgattgc cgccccttct ctctggcaag
    17101 tgaccgagtt tctgcgcggt tgggtactga caggcttccc gtggttacag ttcggctata
    17161 gccagattga tggcccgtta aaagggctgg caccgataat gggcgtggaa gccattaact
    17221 tcctgctgat gatggttagt ggcctgctgg cactggcgtt ggtcaaacgc aactggcgtc
    17281 cgctggtggt ggccgtcgtg ctgtttgccc tacccttccc gctgcgttac atccaatggt
    17341 ttaccccaca accggagaaa accattcagg tttcgatggt tcagggcgat attccgcaat
    17401 cactgaaatg ggatggagat cagcttctta atacgctgaa gatttactac aacgcaacgg
    17461 ctccgctgat gggcaaatca tcgttgatta tctggccgga gtcggcgata accgatctgg
    17521 aaattaatca gcaaccgttc ctcaaagcac tggacggtga gttgcgcgat aaaggtagct
    17581 cgctggtaac cgggattgtc gacgcgcgtc tcaataagca gaaccgctac gatacctaca
    17641 acaccatcat cacgctgggt aaaggtgcac cgtacagcta caaatcagcc gatcgctata
    17701 acaaaaacca tctggtgccg tttggcgagt ttgtcccgct ggagtcgatt ctgcgtccgt
    17761 tagcaccgtt ctttgatctg ccgatgtcgt cgttcagccg tgggccatat atccagccgc
    17821 cgctgtcggc aaatggtatt gagcttactg cggctatttg ctacgagatc attctcggcg
    17881 agcaagtgcg cgataacttc cgcccggata ccgactatct gctgactatc tccaacgatg
    17941 cgtggtttgg taagtctatt ggtccatggc agcacttcca gatggcgcga atgcgtgcgc
    18001 tggagctggc gcgcccactg ttgcgcagca ccaacaacgg cattacggcg gtgattggcc
    18061 cgcagggtga gattcaggcg atgatcccgc agttcacccg cgaggtgttg accactaatg
    18121 tgacaccgac caccggactc acaccgtatg cacgtaccgg caactggccg ctgtgggtgc
    18181 tgacggcgct gtttggtttt gccgctgtgc tgatgagtct gcgagcacgt aaacgttgat
    18241 gtgcctgatg tatcctgcat gagcaaaacg tactttctca ttaagctttg gggcatggtt
    18301 atcatgcccc tttttcttac ggttcaggga tacaagccac attgccatat tggggatgac
    18361 gaataattgc ctgcggatca agcgagcgcg tcctgtccat ttggatcaac ccgttcttcg
    18421 ccatgacaaa tatttttcta ccatccgctg aaaaacgaag gtgattaacg tagctggcag
    18481 gataaacgcc aataatctgc ccggcattaa tattgagcag gatgacataa ccttgcagtc
    18541 catatgacac caacgctaaa tattgaccgt ccgggctaat accctcaacg gaataaacgc
    18601 tcctgctaca ccattgcgtc ataatgctgg tttgttgatc ccctatgctt atacgtgaaa
    18661 tcccctgact atcggcaaaa ataacctcgc cgttttcagg tcgaaaacgc atcgccgata
    18721 tcgttccacg cgcatcacct gcgggaaagt cttctttctg atgcgtgatg gtattgatcc
    18781 gcacgatgtt acctctgcct tctcccggaa ccagtaatgt tctttcatcc ggggaaagtg
    18841 ccataaccca ggcgcctggt ttaccgctcg tccccgtccc gtcgtatttc tccagcggga
    18901 tatccgccac atgctgataa gccaccagat caatggctga cacactatta ctggtgcgat
    18961 tcatcaccca ggcttttgtg ccatcacgcg agaggataag actgtcagga tgtgcaccgg
    19021 tggtaatcga ctgaataatc tcccctgtcg ccacgttaat gaccgataac gcattcgctt
    19081 caaatgaagt cgcataaatc aacttttcat ccgggcttaa tgccagcgat ctgacgccct
    19141 gatctttaca gccaccacaa tgtttgactg acttttcttt cgctatgggc gctgtaaagg
    19201 tatgcaggat tttcttttgt ccgaggtcga aaacaacaat gctgttggtg tcttcaattg
    19261 cggcatacaa cgtattacca tcccgactga tcgccatatc atggatatcg gcaatctcag
    19321 cgagcaaatc accactggcc tgttcacgac ttaccggagc ggataatgtt tccctgaggg
    19381 cgttgcgttc ctccgctaac tgctgctttt tttgccgtcg ctcttgttca atctcctgtg
    19441 gataaaacgc ttcacgcagg ctttgcctga cttgtaaaac tgactcatcc atctgctgct
    19501 gcgcaagcca gatagccccg gtcagaagaa gactgcccgc aacaataatc ccgccttccg
    19561 taacgagtcg gttttgccca gataaacgcg cgctcagcca acccaacgcg ccacccgcaa
    19621 tcaggccgcc aatgtggcaa gcgttatcta ttcctgtttg ccgggcaccg tacaacagtg
    19681 tcagcgccac catacccaac agaggaaata ctcgccgctg gtttttatgg tatgtgcccg
    19741 tacctgaagc aaactgtgtt gctatcgctg caccagctat tcccagaatc gccccggatg
    19801 ccccaacgct gatgactatc tgctcacttt cgcgtaacgc ataacaggcg ctgatgagcc
    19861 caccaaagac gccggagaat aaccaggtaa tcaggagtcg ccatttcccc agaatctgtt
    19921 ccgccacgcc tcccacgacc aggaatgcaa acatattcat cagcagatga gaaacactac
    19981 tgtgcagaaa gatactgctc aataagcgcc agggttcacc cgacaaagta agaggcgcaa
    20041 cgtttgcgcc gagatggata agtgcggatt gatcgacctc ttcataactc atccattgtg
    20101 cgagataaca aaatatccca atacagagtg caaaaaggat aagggttatt gcgggtaatt
    20161 gaacatttaa cggcttaacc gaagatgcag acataaggtg tttcttcctt gaaaagacag
    20221 gtaaaaagca tttttcccgt gtgcagttat catcccggca aacgtcccat gccgaaaaga
    20281 taattctgtc cgaaatatga ctttttcagc ctaaaattac aatcgtaatt aattcgcccc
    20341 taagtatttt ttggcatgta agttgcttta tttactgtgt aagcgatgaa atcagccgcg
    20401 acgcaaccag gttgcaccac cacaaatcag cggtagcacc aaaaaggtgc ggtttaagaa
    20461 ttttgcttcg gaatggtgcg gcgcatctcg caaaaacaaa caataaaaca gcaatatctt
    20521 tacatttagc caaactaaat gttacctaac aggcacaaca ctgcacaata aagttgcaga
    20581 cgataacaac acaaacactc acaacgggta tccatgcgtt cttaacgcag aagataaagg
    20641 agttggatat gcaattacgt aaacctgcca cagcaatcct cgccctggcg ctttccgcag
    20701 gactggcaca ggcagatgac gccgccccgg tagcgggcag tacgctggac aaaatcgcca
    20761 aaaacggtgt gattgtcgtc ggtcatcgtg aatcttcagt acctttctct tattacgaca
    20821 atcagcaaaa agtggtgggt tactcgcagg attactccaa cgccattgtt gaagcagtga
    20881 aaaagaaact caacaaaccg gacttgcagg taaaactgat tccgattacc tcacaaaacc
    20941 gtattccgct gctgcaaaac ggcacttttg attttgaatg tggttctacc accaacaacg
    21001 tcgaacgcca aaaacaggcg gctttctctg acactatttt cgtggtcggt acgcgcctgc
    21061 tgaccaaaaa gggcggcgat atcaaagatt ttgccgacct gaaagacaaa gccgtagtcg
    21121 tcacttccgg cactacctct gaagttttgc tcaacaaact gaatgaagag caaaaaatga
    21181 atatgcgcat catcagcgcc aaagatcacg gtgactcttt ccgcaccctg gaaagcggtc
    21241 gtgccgttgc ctttatgatg gatgatgccc tgctggccgg tgaacgtgcg aaagcgaaga
    21301 aaccagacaa ctgggaaatc gtcggcaagc cgcagtctca ggaggcctac ggttgtatgt
    21361 tgcgtaaaga tgatccgcag ttcaaaaagc tgatggatga caccatcgct caggtgcaga
    21421 cctccggtga agcggaaaaa tggtttgata aatggttcaa aaatccaatt ccgccgaaaa
    21481 acctgaacat gaatttcgaa ctgtcagacg aaatgaaagc actgttcaaa gaaccgaatg
    21541 acaaggcact gaactaatta caagaaccag gggcggaaat tccagccctc tcgattgtta
    21601 cgtagcacgg acagactata cgcctgatgg tcgttcccca tcgggcctga aaaccgcaat
    21661 acgctgggta acaatcttcg agggtagcag ttaacgctgc taccctcttt tttctggagt
    21721 agatttatgt ctatagactg gaactggggt atttttttac aacaagcccc gttcggcaac
    21781 accacctatc ttggttggat ctggagtggt tttcaggtga cgatcgcttt gtcgatctgc
    21841 gcctggatta tcgctttcct cgtcggttca tttttcggca ttttacgtac cgttccaaac
    21901 cgttttctct ccggtctggg tacgttgtat gtcgaactgt tccgcaacgt gccgttgatt
    21961 gtgcaattct ttacctggta tctggtgatc ccggagctgt tgccggagaa aatcggcatg
    22021 tggtttaagg ccgagctgga tcccaatatt cagtttttcc tttcatccat gctctgcctg
    22081 gggctgttta ccgccgcccg tgtttgcgaa caggtacgcg ccgctattca gtcgctcccg
    22141 cgtgggcaga agaatgccgc gctggcgatg ggcctgacgc taccgcaggc ttaccgctat
    22201 gtactgctgc ctaatgctta tcgcgttatc gtcccgccga tgacctccga gatgatgaac
    22261 ctggtgaaaa actccgccat cgcctcgact atcggtctgg tggatatggc agcgcaggcg
    22321 ggtaaattgc tcgactactc ggctcacgcc tgggagtcgt ttaccgccat tacgctggcg
    22381 tatgtattga ttaacgcggt tatcatgctg gtgatgacgc tggttgaacg taaagttcgc
    22441 ctgcccggca acatgggggg caaataatgt acgagtttga ctggagttcc attgttcctt
    22501 ccctgccata tctgctcgac gggctggtga tcacgctgaa aatcaccgtc acggcagtcg
    22561 tgattggtat tttgtggggc accatgctgg cggtgatgcg cttgtccagc tttgcgcccg
    22621 tagcgtggtt tgccaaagcc tacgttaacg tattccgctc gattccttta gttatggttt
    22681 tgctgtggtt ttacctgatc gtgccgggtt ttctgcaaaa cgtgctggga ttatcgccaa
    22741 aaaatgatat ccgtttaatt tcggcgatgg tggcgttttc gatgtttgaa gcggcctact
    22801 attcagagat tatccgtgct ggtattcaaa gtatttctcg tggtcagtcg agcgccgcgc
    22861 tggcgttggg aatgactcac tggcagtcga tgaaactgat tattctgccg caggcgttcc
    22921 gcgcgatggt gccgcttctg ctcactcagg gcatcgtact gttccaggat acctcactgg
    22981 tgtatgtgtt atctctggcc gatttcttcc gtaccgcctc aaccattggt gagcgtgatg
    23041 gtacgcaggt tgagatgatc ctgtttgccg gatttgttta tttcgttatt agtcttagcg
    23101 cgtcgttgtt ggtcagctac ttgaaaagaa ggacagcata atgattaccc tgaaaaatgt
    23161 ttcaaaatgg tatggtcact ttcaggtgct gaccgactgc tcaaccgaag tgaaaaaagg
    23221 cgaagtggtg gtggtttgcg gcccgtctgg ctccggcaaa tcaacgctga ttaaaaccgt
    23281 caacggcctc gaaccagtgc agcaaggtga aatcaccgtc gatggtatcg tggttaatga
    23341 caagaaaacc gatctggcaa agctgcgttc ccgcgtcggg atggtgttcc agcattttga
    23401 gttgttccct catctgtcga ttatcgaaaa cctgaccctg gcgcaggtga aagtgcttaa
    23461 acgcgataaa gcgccggctc gtgaaaaagc attgaaattg ctggagcgcg tgggactttc
    23521 cgcacatgcc aataaatttc cggcgcagct ttccggcggt cagcagcagc gtgtggcgat
    23581 cgctcgcgca ttgtgtatgg atcctattgc gatgctgttc gacgaaccga cgtcggcgct
    23641 ggatccggag atgatcaacg aagtgctgga cgtgatggtg gaactggcga acgaaggaat
    23701 gaccatgatg gtggtgaccc acgaaatggg ctttgcccgc aaagtggcga atcgggtgat
    23761 ctttatggat gagggtaaaa ttgtcgaaga ctcgccgaaa gacgctttct tcgatgatcc
    23821 gaaatcggac cgcgcaaaag acttcctcgc gaaaatcctg cattaatcac tctggcgcgc
    23881 atacccaagt gcgcgccatc tcacgcttta tcgtttcgcc cctcgtatct gttacatcct
    23941 ggcgttaccc ttgtcgcaaa gaagcacaca acaaggagca acaatggcac tgccaattct
    24001 gttagattgc gacccaggtc atgacgacgc tatcgcaata gttctcgccc tcgcctcacc
    24061 agagcttgat gtcaaagcaa ttacgtcttc cgccggaaac cagacaccag aaaaaacctt
    24121 acgcaatgtt cttcgtatgc tgaccttgct caatcgcacc gatattccgg tagcaggcgg
    24181 cgcggtaaaa ccgttaatgc gtgagttgat tatcgcggac aatgtgcacg gcgaaagcgg
    24241 tctcgacggc ccggcattac cggaaccggc gttcgcaccg caaaactgta cggcggtaga
    24301 gctaatggcg aaaacgctgc gtgaaagtgc ggaacctgtc acgattgtgt ctaccggacc
    24361 gcaaactaac gttgccttgc tgctcaatag ccatccggaa ctgcatagca aaattgcccg
    24421 tatcgtgatt atgggcggcg caatggggct tggtaactgg acgcctgcgg ctgaatttaa
    24481 tatttacgtt gacccggaag cggcagaaat tgtcttccag tcagggatcc cggtggtgat
    24541 ggccggtctg gatgttactc ataaagcaca aatccacgtt gaagacaccg agcgtttccg
    24601 cgcgattggc aaccctgttt caaccattgt tgccgaactg ctggatttct tcctcgaata
    24661 tcataaagac gaaaaatggg gctttgtcgg tgcaccgctg catgacccat gcaccatcgc
    24721 ctggctgttg aaacccgagt tgtttaccac tgttgagcgc tgggttggcg tggaaacaca
    24781 ggggaaatat agccagggta tgacggttgt tgattattat tatctgactg gcaataaacc
    24841 gaatgccact gtaatggtcg atgttgatcg tcagggcttt gttgatttac tggctgatcg
    24901 tctgaaattt tacgcttaac acacgtcatt gattaagatc accacgcgtt acctgggtgg
    24961 tcttaatctg tttatctctt tttctttcaa gatataaatg caatggataa tgcagaactc
    25021 gccattggta tcgatctcgg cactaccaat agtttaattg ccgtctggaa agacggtgcc
    25081 gcgcaattaa ttccaaataa gttcggtgaa tatttaacac catccataat tagcatggat
    25141 gaaaataatc atattttagt cggaaaaccg gctgtatcac ggcgtacttc gcatccggat
    25201 aaaacggcag cgttatttaa acgtgcaatg ggcagtaata ccaactggcg gttaggcagc
    25261 gacacattta acgcgccaga actgtcctct ttggtattac gctcattaaa agaagatgcc
    25321 gaagaatttc tgcaacgtcc gattaaagat gtggtgatct ccgttccggc ttatttcagc
    25381 gatgaacaac gcaagcatac ccgtttagca gcggagttag ccgggttaaa tgcggtacgc
    25441 ttaattaatg aacccacagc agctgcgatg gcatatggcc tgcatacgca acaaaatacc
    25501 cgctcgctgg tttttgatct cggtggcggc acgtttgacg tcacggtgct cgaatacgcc
    25561 acgccggtga ttgaagttca cgcctccgct ggcgataact tcctcggtgg cgaagatttt
    25621 acccatatgt tggtcgatga cgttttaaag cgcgcggatg tcgccaggac cacgcttaac
    25681 gagagtgaac tggcagcctt gtacgcctgt gtggaagcgg caaaatgtag caatcaatcg
    25741 ccattgcaca ttcgctggca gtaccaggat gaaacgcggg agtgcgaatt ttacgagaac
    25801 gaactggaag atttgtggtt gccgctgctc aatcgcttgc gagtgccgat tgaacaggcg
    25861 ttgcgcgatg cacgtctgaa gccgagtcaa atcgacagtc tggtgctggt tggcggcgcg
    25921 tcacaaatgc cgctggtgca gcgaatcgcc gtgcgtctgt ttggcaaatt accgtatcaa
    25981 agttacgacc cgagcaccat tgtcgcgctg ggcgcagcaa tccaggccgc ctgccgctta
    26041 cgcagtgaag atattgaaga ggtgatcctc actgatattt gcccttactc gttgggcgtt
    26101 gaagttaacc gccagggcgt ttccggcatt ttctcgccaa ttattgaacg aaacaccaca
    26161 gtacccgtgt cgcgtgtaga aacttactcg accatgcacc cggaacagga ttcaattacg
    26221 gttaacgtct atcagggaga aaaccacaaa gttaaaaata acattctggt tgaatccttc
    26281 gatgtgccgt tgaagaaaac cggggcttat cagtcgattg atattcgctt tagttatgat
    26341 atcaacggct tgcttgaagt tgacgtgctt ctggaagatg gcagcgttaa gtccagagtc
    26401 attaatcata gcccggtaac attgagcgcg cagcagattg aagagagtcg gacgcggtta
    26461 tccgcattga aaatttatcc gcgcgatatg ctcatcaacc gcacctttaa ggccaaactg
    26521 gaagagttgt gggcgcgggc gctgggtgac gagcgagaag agattggccg ggtgatcacc
    26581 gattttgatg cggcgttgca gtcaaacgat atggcccgcg tcgatgaggt caggcggcgg
    26641 gcgagcgttt atttagccat tgagacctca taatcacggc tgccggatgc ggcgtaaatg
    26701 ctgcatccgg catcggcgca aaaaacacat cgtcaactaa acaacatcgc gattttgccg
    26761 agagcataac ctataaccag caccgttaaa ctgattttga taatgctggt aatgctctgt
    26821 ggtttttcac cctcatcatt tttttcttca tggttttcag gtgttggctc atcgacatct
    26881 tcatcctccg gctcgagttt tactttcgtc aacagcatcc gggtgataat ggcctctaat
    26941 ggatgagcat ctccgccttc cagccttgta ttaagacggg ccagcatctc ttgtttcgga
    27001 aacgcttcaa gtactacctg ttccgctacc cgatacaaat ccagtaacgg tgaatcttct
    27061 tctgtctcgc taccgactct cgatttccac cacgcccatg ctttgcgttt gaagaataac
    27121 gactcaataa acgattgacc taacggcgat aattttccgc tatgtaaaat ttccgcccat
    27181 aacagccgcg tccgggcgct ctcaccagga acatatttca gcggttgacc ttgcgcatct
    27241 accatgctcc gcgcttcgat aaccatcact aataacgcga aataatccgg gcaatgactc
    27301 ttgctccact gcaacaacaa attatcagct tgtgtagatg ggtaactttc ccggtacgcg
    27361 cagagatcgg caagcaggct ttccccctcg ccgcagtaca acctttgagc acacaagtaa
    27421 tatcgggcat cttcgctttc tggttcttcc atttgccact gtttggctac aggaatgagt
    27481 tccgccatac ctgtttgcac cgagctgtac cagcgcaaca gtttgcgatg taactttgca
    27541 tcatcaggaa tgaaccacgg accttccatc gccaaaaacg cagtgacgta gtcaggatgg
    27601 taacggcagc aacgttccag cgcgaagtaa tactcaacag tctggttctg caacgcaacg
    27661 ggatattcca gaaggctaag gaaatcgaaa caatcaccag cctgaatggc ctcaagaaaa
    27721 gcctcccttt cttcctcatc cattccttcg ctgtcattaa acgaatgcca gttcaaacgc
    27781 tctgccagca aagaagcaca atttaatgaa agatatcgcg cttctatagc gattgcacac
    27841 agcggccagc gtaattgatc gacatcctcc atgttccagg tatttaattg ctgaataaat
    27901 ttctgccagg cggagggctg aaaacgatca ctttctgaat ccagtaatgt cctgaatgca
    27961 cgcagtattt catgttcagc ggcggcatct ttttcttcat catctgcttc ctcgacagga
    28021 tttaccgcca gtcgcagcgc ctcttcatag gcctggcgta atgctttaaa cccctgcgga
    28081 tcggtttcgg gatgacataa cggtaagcga gcaagataag cctggcggat aatgtctatt
    28141 tgcgtcgtgc tttcaatttc gaggatttgc caacatgttt tcataggtcg ctcacatcct
    28201 taagcgttac cgggcattca ggctggacag ttaatggctg ccagggaagc gtggcataga
    28261 aacgatcgct tcccgctgcc agctcttcga acatccgact cccggcgcta tcgtatttgt
    28321 acagggttag cgcctcgcgt agcgaagtgt tgtcttcgca ctgagaagat tgccagtaga
    28381 gacgtcctaa cgaataggcg gaaaaatagt gcatccagct gtgatagtaa tggtgagctc
    28441 gtacatagac gcgcgattgc agccataaac tatcttcttc cgtcagccag ttgttgagca
    28501 caccgacccg tgacagaaac cccattcgaa cataatccca ggctttgatt cccccttctc
    28561 cacagcacat agcggtttgg gcaacgaatt gggtataaat caaccctctc tccgagccac
    28621 cagcaattaa tgctttccac tcttcagggg aagagcgatg ccatgtcaga tataagccag
    28681 ctaaatcatt ggcatgacca tcatcagtca tctgataaat agtctgaatt aaatcatcac
    28741 gattatttat tccccaggat gatgagagat cgaattcttt tataccggta tacatttttg
    28801 gcgagtcatg cgcgggtttg caaccctgat taagtatttc catgggcgca gaaagcgcaa
    28861 aaagaagata ttgttcttcc ttattcattt gttaataatc ccttaagtgc attaactgac
    28921 tactttttat tattgatgag tataaacttg acgctttcaa aataaaaaga aaatcgaagt
    28981 attcacatat gaataaaaaa ttaatgtata tattcgcaat atttatagtt gcagcaatta
    29041 cctgtattag ccaacccaag aaaacaacgt tgcgtgataa agccatggtg aattatgcct
    29101 ttgattattt aagctcacca ggcagtctac cgttaaccac ggcagccacg gagctttccg
    29161 cgattcatgg tcactcaaca tcgcaatatc gccttggcga attttatctt catggtagcg
    29221 acggtaaacc actggattat acacaggcga gatactggta tgagcaatca gcagaacagg
    29281 aaaatccgcg cgcacaaagc aaactggggt ggatctacct gaaaggcctg ggagtcaaac
    29341 ccgatacccg taaagcaatt ctctggtata aggaagcagc tgaacaaggg tatgctcatg
    29401 ctcaatatac tttgggtttg atctacagaa atggcactgg tattaatgtt aaccattatg
    29461 aatctcaaaa atggttaaaa ctggccgcca aacaacatta caaaaatgcg gaaagattac
    29521 ttgccgggct tcccgcacat taacgtcgac tgtcaaccga acagatggag tattttccct
    29581 atcaaaccag caaagataaa aatatagaaa ataattttaa tgatgcccag acagcctggc
    29641 ccaccatcgt caacgggcat tggctgcggt tgcggctccg gttcaacagt ttgcttctcg
    29701 gtaaaattcg ccgcctgaat taataatcga cgaataatta atgcttccag tggacaacga
    29761 tcttctgcat caaggcggct cacaatcggc attaatgatt cttcggttgg ttggttcgcc
    29821 tgcactaatt gtttggcaac taaatataat ggccataagg gatgttctgt atcacgcgtg
    29881 agtgagatcc gcggcttacg tcctttgtgc aaaaccatct cgaccagtga acgccccaga
    29941 gcagagagtt tttcatcgct gagtatttcg tataaatgaa aacgcgtacg ggcgctgtcg
    30001 ccggggatat aaagtagcgg ttttccctga tcgttaacca gttcacgagc ttcaatcatc
    30061 atcaccacta acgggtaata atcgacccga tgctgacggc accattgcaa tattaaagca
    30121 tccgcctggg tggagggata ctcgcgccag tagtcacaaa gttctgccag caagctttcg
    30181 ccttctccac aatagacgcg ttgtgcgtat tcataataac gcgcatcttc attatcagat
    30241 tcagcttccc gccacgaccg ggcgtaatcc aggagttcgg gaattcccag gcgacctgcg
    30301 ctataccagc ggagtaaatt tctgtgcagt tttgcatcat cggggataat cactgcgcga
    30361 tgttgcgcta aatacaatgt cagccattct ggatgcgatg accaaatccg agcgaacatt
    30421 tggtaaaaat cgatggtgtc attttgcacg gcaacgggca gatgcagaat ggtctggaag
    30481 ttaaaaacat tccccttcgc aatggcatat aaaaaggatt ccagttcttc ttcgtctatt
    30541 tccccgacgt tttcctcctg caaccaccgc aatctttctg ctaataacac cacgcactcg
    30601 aaggataaat gggcagtgtt catggcgatt gtgcacagcg accagcgtaa ttcatcaatc
    30661 tcttccatcg agcaataatt taattgctga atgaatcgct gccaggcgga gggcagaaaa
    30721 cgttcgctat cagaggcaag taacgcacga aaggcgagca gaatttcatg ttctgctacc
    30781 tcatattctt ctggttgcca aacagattta gcaggcgact gcgcaatccg tagcgcctcc
    30841 tcatacgcct gacgaagttg tttaaaaccc tgcggatcgg tttctggatg aaaggacggt
    30901 aacagcgcca gataagcgcg gcggataata tcgacgtcag tcgtttcctc tatttcgaga
    30961 atcttccagc aatttttcat aggtcgctca tatccttaag cgtttccggg catgcagaat
    31021 agtaagccaa tggttgccag gacaaggtag ggtaatacgc atcatcactg gcaaataatt
    31081 gataaaacat atcattgccg gaatcgtcgt attctttacg cgctaagaat tcgcgtaata
    31141 actgcagatg atcatcttcg ggagactgcc aatattgccg accaaaggta taaccggcaa
    31201 agtattgccg ccagttacga taataacgta gagcccgtag atgaatgcgc gactggatcc
    31261 atagactttc ttcttcgctc aaccagttat taagtacgcc cattcggctt aaaaaaccca
    31321 tccggacata atcccaggct ttaattcctc cctcaccaca gcattcggcg gtgctggcga
    31381 caaattgcgc ataagcctga ccttgctcat ttagttcagc aagatagtca cgccattcac
    31441 atggcgaata acgaaaccag cggtgataaa accctgccaa ccgagcggca tgaccgttat
    31501 cgatcattcg gtgaattgtg cgcagcaggt catcacgggc atcaattccc caggaggaag
    31561 aaaggtcaaa aaagcatgta cctaaataca ttttgggtga tgaatgcgaa ggtaaacagc
    31621 attcattgcg tatttccatc ggagtggaaa gtgcaaacaa tattttttgc gac
//
LOCUS       DAZFXT010000050        30966 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.050, whole genome shotgun sequence.
ACCESSION   DAZFXT010000050 DAZFXT010000000
VERSION     DAZFXT010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30966
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.050"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(14..1900)
                     /gene="prpE"
                     /locus_tag="ACHKW0_002430"
     CDS             complement(14..1900)
                     /gene="prpE"
                     /locus_tag="ACHKW0_002430"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050218 - propionate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HFO7475250.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRHQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(1940..3391)
                     /gene="prpD"
                     /locus_tag="ACHKW0_002431"
     CDS             complement(1940..3391)
                     /gene="prpD"
                     /locus_tag="ACHKW0_002431"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047547 - 2-methylcitrate dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019679 - propionate metabolic process,
                     methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HFO7475251.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTAMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(3425..4594)
                     /gene="prpC"
                     /locus_tag="ACHKW0_002432"
     CDS             complement(3425..4594)
                     /gene="prpC"
                     /locus_tag="ACHKW0_002432"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050440 - 2-methylcitrate synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HFO7475252.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVENKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRPFVALDKRQ"
     gene            complement(4639..5529)
                     /gene="prpB"
                     /locus_tag="ACHKW0_002433"
     CDS             complement(4639..5529)
                     /gene="prpB"
                     /locus_tag="ACHKW0_002433"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046421 - methylisocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HFO7475253.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            5768..7354
                     /gene="prpR"
                     /locus_tag="ACHKW0_002434"
     CDS             5768..7354
                     /gene="prpR"
                     /locus_tag="ACHKW0_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HFO7475254.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(7452..7727)
                     /gene="yahO"
                     /locus_tag="ACHKW0_002435"
     CDS             complement(7452..7727)
                     /gene="yahO"
                     /locus_tag="ACHKW0_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family periplasmic protein YahO"
                     /protein_id="HFO7475255.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            7874..8545
                     /gene="yahN"
                     /locus_tag="ACHKW0_002436"
     CDS             7874..8545
                     /gene="yahN"
                     /locus_tag="ACHKW0_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HFO7475256.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(8562..8807)
                     /gene="yahM"
                     /locus_tag="ACHKW0_002437"
     CDS             complement(8562..8807)
                     /gene="yahM"
                     /locus_tag="ACHKW0_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahM"
                     /protein_id="HFO7475257.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(9220..10035)
                     /gene="yahL"
                     /locus_tag="ACHKW0_002438"
     CDS             complement(9220..10035)
                     /gene="yahL"
                     /locus_tag="ACHKW0_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YahL"
                     /protein_id="HFO7475258.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNQWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(10278..11327)
                     /gene="yahK"
                     /locus_tag="ACHKW0_002439"
     CDS             complement(10278..11327)
                     /gene="yahK"
                     /locus_tag="ACHKW0_002439"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HFO7475259.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKYAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(11414..12370)
                     /locus_tag="ACHKW0_002440"
     CDS             complement(11414..12370)
                     /locus_tag="ACHKW0_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7475260.1"
                     /translation="MKKSWRNNVEFYLIGLLALTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQARGMLGLDRVV"
     gene            complement(12367..13338)
                     /locus_tag="ACHKW0_002441"
     CDS             complement(12367..13338)
                     /locus_tag="ACHKW0_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7475261.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(13331..14815)
                     /locus_tag="ACHKW0_002442"
     CDS             complement(13331..14815)
                     /locus_tag="ACHKW0_002442"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HFO7475262.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(14864..15847)
                     /locus_tag="ACHKW0_002443"
     CDS             complement(14864..15847)
                     /locus_tag="ACHKW0_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7475263.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(16102..16512)
                     /locus_tag="ACHKW0_002444"
     CDS             complement(16102..16512)
                     /locus_tag="ACHKW0_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HFO7475264.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTASCGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(16811..18193)
                     /gene="yahJ"
                     /locus_tag="ACHKW0_002445"
     CDS             complement(16811..18193)
                     /gene="yahJ"
                     /locus_tag="ACHKW0_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HFO7475265.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVTAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(18203..19153)
                     /gene="yahI"
                     /locus_tag="ACHKW0_002446"
     CDS             complement(18203..19153)
                     /gene="yahI"
                     /locus_tag="ACHKW0_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HFO7475266.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(19296..20714)
                     /gene="yahG"
                     /locus_tag="ACHKW0_002447"
     CDS             complement(19296..20714)
                     /gene="yahG"
                     /locus_tag="ACHKW0_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7475267.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(20714..22261)
                     /gene="fdrA"
                     /locus_tag="ACHKW0_002448"
     CDS             complement(20714..22261)
                     /gene="fdrA"
                     /locus_tag="ACHKW0_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7475268.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIVLISKPPAPAVARKVLERARACRK
                     PVVVCFLGRGETPVDEQGLQFARGSKEAALKAVMLSGVKQEHLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(22251..23114)
                     /gene="yahE"
                     /locus_tag="ACHKW0_002449"
     CDS             complement(22251..23114)
                     /gene="yahE"
                     /locus_tag="ACHKW0_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7475269.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWQQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(23154..23759)
                     /gene="yahD"
                     /locus_tag="ACHKW0_002450"
     CDS             complement(23154..23759)
                     /gene="yahD"
                     /locus_tag="ACHKW0_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HFO7475270.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            24017..24514
                     /gene="yahC"
                     /locus_tag="ACHKW0_002451"
     CDS             24017..24514
                     /gene="yahC"
                     /locus_tag="ACHKW0_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7475271.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGSKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            24606..25538
                     /gene="yahB"
                     /locus_tag="ACHKW0_002452"
     CDS             24606..25538
                     /gene="yahB"
                     /locus_tag="ACHKW0_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7475272.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFYAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(25580..26668)
                     /gene="pdeL"
                     /locus_tag="ACHKW0_002453"
     CDS             complement(25580..26668)
                     /gene="pdeL"
                     /locus_tag="ACHKW0_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7475273.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(26810..30793)
                     /gene="ehaA"
                     /locus_tag="ACHKW0_002454"
     CDS             complement(26810..30793)
                     /gene="ehaA"
                     /locus_tag="ACHKW0_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HFO7475274.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKAIDFGKENQSIDYRITVTDNANLVINTTDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDQGYFNVSNGGKIH
                     VKGTSRLTYTQGNVSGEGSQVNAGTFFMGVYGGYGGNQFLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITKNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVAVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSLVTVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKDQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNGDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANRGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTTMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGNQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT         7382 a         8374 c         7439 g         7771 t
ORIGIN      
        1 atccgacaat accttactct tccatcgcct ggcggatctg atccaatgac gcaggatcat
       61 caatggtcgt cagatcccca ggatcgcgtc cttcgcaaat tgcctggatc gtgcggcgca
      121 gcatttttcc ggatcgcgtt ttcggcaatt gcgagacaaa ccagacgtgc gccgggcgac
      181 caaagttgcc aatctggctg tccaccagcg ccataatcgc cttctcttgc gaatgcgcca
      241 catcacgatc ttccagacta tcgctctctt tcggaatgac aaacgccacc gccacctgcc
      301 ctttcagcgc atctttcacc ccaaccaccg ccacttcggc aacgcccgga tggctggaga
      361 tactctcttc aatctcgcgc gtgcccagac gatgcccggc aacgttaatt acatcgtcag
      421 tgcgcccgag aataaagtga taaccgtcag cgtcacggat gccccagtca aaggtggcgt
      481 acaccgggcg ggaaaacagc gaccagtaag tcttcacaaa gcggtcgtcg tcgccccaga
      541 tggtctgaat acagcccggc ggcagcggcc cttccaccac cagcatccct ttctcgttga
      601 cgccacacgg ttcgccggtg acttcattaa gcaactgcac gttatagcca tacatcggca
      661 cgccggggct tcccagacgc gtcggcctgt cgtccagacc gcgagcaatc gccataatcg
      721 gccagccgga ttcggtctgc cagtagttgt cgatgaccgg cacatccagc gtattgctca
      781 cccaactggc ggtcggctcg tccagcggtt ctccagccag atagagcact tccagcgacg
      841 agagatcgtg tttgcgaatt tcagcggtag ggaatttttt cagcacgcga atggcggtcg
      901 gcgctgagaa catccggcta acctgatatt tctcgacgat tttccaccac acgccgcagt
      961 ccggccaggt cggcaatcct tcgtaaacga tagtcgccat ccccgccagc agcggcgcgt
     1021 aaacgatata cgaatgcccc accacccagc cgatatccga tgcgcaaaag aacacgctgc
     1081 ccgctttgcc gccaaaaatg gtgtccatcg aggtcgccag cgccaccgca tatccgccga
     1141 cgtcacgctg cacgccttta ggtttgccgg tcgtgccgga agtgtagaga atgcaggagg
     1201 tttcgttgga ttccagccac gccaccggta cccgcgcgcc gatgtgttga tggcgcaacg
     1261 acgcgaaatc gacatcccgc ccgctgacgc gcgccatttt cgccagcccg cgatccacca
     1321 gcaaaacatg gcgtggctgg tgctgcgcct gacttatcgc atcgtcgagc aattttttat
     1381 agggaatgat tttgccaccg cgcgctccgg catcagccga gacaatcagc accggtttag
     1441 cgtcatcaat tcgcgccgcc acgctgtgcg aggcaaaacc accaaatacc accgaatgaa
     1501 tagcaccaat gcgcgcgcag gccagcagag taatatgcgc ttcggcaatc atcggcatat
     1561 acaccagcac ccgatcgccg cgctgcacgc ccagtgagcg caacattgag gccaccgcgt
     1621 tcacttcgtc atgcagctgg cgaaaggtaa aggtgcgctc ttcttctgtt tccgaagaga
     1681 cggcaatcaa tgccagtgcc tctggctgtt tctccagcca gcggtcgata gcgttgtggc
     1741 acaaattggt tcggccttca caaaaccaac gggcaaacgg cggattgctg tggtcgagcg
     1801 tttgcgtaaa gggcgtctgc cagtcaatac gccgggcctg ctcggcccag aactgctccg
     1861 gttcgttaat cgaacgctga taaaattcgc taaaagacat aatgctctcc tgttgaactt
     1921 acgccttacc gccgtttact taaatgacgt acaggtcgag atactcattg accggcatct
     1981 gttccaggcg agttctgtcg agagaaacct ccagaatgcg ctgctgttgg cgagtcggga
     2041 actggcgcgc gagattgatt ttgaatttat cgaccagttt cggaatacca tcctgacggc
     2101 ggcgagcatg accaatgggg tactccacca ccacttcttc aaatcgtgtg ccgtcggtga
     2161 actcaagggt aatggcattg gcgatggcgc gtttttccgg gtcgtggtag tcagcggtaa
     2221 atgccggatc ttcaaagcaa ttgatcttct cgcgcagggc gtcgatgcgt ttatcttgcg
     2281 caacgttgtc ctcgtaatct gccgccgtta agcgcccgaa taacagcggg atcgccacca
     2341 tgtactgaat gcagtggtcg cggtcagccg ggttattgag cggccccttt ttgtcgatga
     2401 tgcgaataca ggcttcgtgg gtgcgaatgg tcactttttc gatatccgcc gccgtcttgc
     2461 ctgctgcctg catctgttca tagagcgtca tcgccgcttc aactgccgtc tgggagtgga
     2521 actccgccgg gaaggagatt ttgaacagca cattctccat gacgtaagaa ccgtacggac
     2581 gctggaagcg gaacgattca cctttaaagg agacgtcgta gaagccccat accggcgcag
     2641 tcagggctga cgggtaaccc atttcgcccg ttttcgccat cagtgccaga cgtaccgcgc
     2701 gggaagtggc atcgcccgcc gcccaggatt tacgcgtgcc ggtgttcggc gcatggcgat
     2761 aggtgcgcag cgactgaccg tccacccacg ccagcgaaac ggcgttgaga atttcctcgc
     2821 gggtcaggcc gagcatttcg gcgaccacgg cggtggaagc cactttcact aacagaacgt
     2881 ggtcgaggcc gacgcggtta aaggagtttt ccagcgcgat gcagccctga atttcatggg
     2941 ctttgatcat tgcggtcagc acctgtttca tggtcaacgg cgctttgccg ctggcgaccg
     3001 cgttgcgcga aagccagtcc gccgttgcca gaatgccgcc gaggttgtcg gaaggatggc
     3061 cccactccgc cgccagccag gtatcgttga aatcgagcca gcggatcatc gcgccgatgt
     3121 taaatgccgc ctggacgggg tcgagctgga actgagttcc ggggacgcgc acgccgttgg
     3181 gtacgacggt gccgggaaca attggcccca gcagtttttt acaggccggg tattcgagag
     3241 cttccagacc gcagccgagc gtgtcgagca ggcagtaatg tgcggtgtcg taggccactt
     3301 tagagctgat ttcgtagttc atgacgtaat cgacgatatc aacgatttca cgatcaaatt
     3361 ccgggcggat gttgttgatt tgagctgaca ttgggtacgt ttccttattg ttattcgtag
     3421 aggtttactg gcgcttatcc agcgcgacaa acgggcggtc ttccggtcca acataattgg
     3481 cggaaggacg gataattttg ttgtcctgac gttgttcgat aatgtgcgcc gcccagccgg
     3541 tgacgcgggc gataacaaac agtggtgtga acatctcggt gggaacgccc atcatgttgt
     3601 aggaaacagc agagaaccag tcgagattgg ggaacatctt tttgctctcc cacatcaccg
     3661 tttccaggcg atcggcgatg ttgtacatct tcagcgagcc gccttcctgc gagagctgct
     3721 tcgccacacg tttaatcacc tggtggcgcg ggtcagcgat ggtgtaaacc ggatgaccaa
     3781 aaccaatgac cacttctttg ttttccacgc gcttgcggat atctgcttcg gcttcgtccg
     3841 gcgtttcgta gcgttgctgg atctccagcg acacttcatt cgccccgccg tgttttggcc
     3901 cgcgcagtgc gccaatcgcg ccaataatcg cggaatacat atcagagccg gtgcccgcaa
     3961 tcacccgact ggtaaaggtg gaggcgttaa actcgtgttc ggcgtacagc accagcgaga
     4021 tatgcatcgc cttttcccag ctttgcgatg gcttttcgcc gtgcagcaga tgcaggaagt
     4081 gaccgccgat ggagtcgtca tcggtttccg gctggatgcg ttcgccgttg tgactgtagt
     4141 gataccaata aaggagaatc gagctaagtg atgccagcag tttgtcggca atatcccgcg
     4201 cgccagagac agtgtgcccc tctttttctg gcagtgtgca gccgagcgcg gaaacgccgg
     4261 tgcgcatgac atccatcggg tgcgacgccg ccggtaaggc ttccagcacg gtacgcacat
     4321 tagctggtaa accacgcagg gctttcagtt tcgttttgta agcggcgagt tcgtcacggg
     4381 tcggcagttt gccgtggatc agcaggtgcg ccacttcttc aaattcgcaa tgttccgcca
     4441 gatcaagaat atcgtagcca cggtaatgca ggtcattgcc acttttaccc acggtgcaga
     4501 gcgccgtatt gcccgccgga acgccagaaa gtgctaccga ttttttcggt ttaatgacat
     4561 gggtactgtt ttgcaggatc gttgtgtcgc tcatattgtc ctcgtcattt gtagggtcga
     4621 gatatttttc gttgtgtttt atttcacctg gctacgggca aacaggtcgt cgagcttctc
     4681 ttcgtactgg tagtagttga tgctttcgta cagctcgttg cgggtttgca tggtatcgat
     4741 gacgcttttc tgcgtgcctt cctgacgcag gacgttgtag acatgttcag cggcgcggtt
     4801 catggcgcgg aacgctgaaa gcgggtacag cgccattgcg acatgggcgc tgcgtaattc
     4861 gtcggtggta aacagcggtg ttgcgccaaa ttcggtaatg ttggcgagga ttggcacctg
     4921 caccgcatcg gcaaactggc ggtacatggc gagttcggta atcgcctccg ggaacaacat
     4981 ctcggcaccc gcttcaacat aggcctgcgc acgctcgatc gccgcatcca gcccctctac
     5041 cgccagagca tcggtgcgcg ccatgatcac aaaatcagga tcggttttcg catccaccgc
     5101 cgcgcggatc cgatccacca tctcttcttt cgagacgatc gctttattcg gacgatgacc
     5161 gcagcgtttc gcaccaacct gatcttcaat atgcaatcct gccgcaccgg ctttaatcat
     5221 cgatttcacg gtgcgcgcca cgttaaaggc cgaagaacca aaaccgatat ccgcatccac
     5281 cagcagcggc agcgaacaaa cgtcggtgat acggcgaatg tcggtcagca catcatcaag
     5341 ggtagaaata ccgagatcgg gcagccccag cgaacctgcc gccacgccac cgccagaaag
     5401 ataaattgcc tgatatccgg cacgctgcgc caacagcgca tgattagcgt tgatggtgcc
     5461 aacaatctgc aatggatttt ctttagtcag tgcagcgcga aacgctttac ctggagagtg
     5521 tagagacata gcccatcctt tgttatcaac ttgttatttg cgttgataaa gacaaagcaa
     5581 ggggtgtgcc acaattgcct taataaaatt aaatgtttaa tttcaatttg ttagagataa
     5641 agtcatgaac taatccgcaa atatgcgttt cagttaacgt ttcaggcaat gtttcaccgc
     5701 gtttcattgc aacaattatg aaacaagact aaacccaata ttcggtttct taactttgcg
     5761 gtgcgctatg gcacatccac cacggcttaa tgacgacaaa ccggttatct ggacggtatc
     5821 tgtaacgcgc ctgttcgagc tgtttcgcga tatcagcctc gagtttgatc acctggcgaa
     5881 cattacccct attcagcttg gctttgaaaa agcagtgacc tacatccgca agaaactggc
     5941 aaacgaacgc tgtgacgcca tcatcgcggc tggctctaac ggcgcgtacc tgaaaagccg
     6001 cctgtcagtg ccagttattt tgattaaacc gagcggctac gatgtgttac aggcactggc
     6061 aaaagccgga aaactcacct cttctatcgg cgttgtcacc tatcaggaaa ccattccagc
     6121 gctggtggcg tttcaaaaaa cctttaattt gcgcctcgat caacgtagct acattaccga
     6181 agaagacgca cgcgggcaga ttaacgagct aaaagctaac ggcaccgaag cggtggtcgg
     6241 cgcggggctg attaccgatc tggcagaaga agccggaatg accggaattt ttatctattc
     6301 cgccgccacc gtgcgccagg cgttcagcga tgcgctggat atgacgcgca tgtcgttacg
     6361 ccataacact cacgatgcca cccgcaacgc cctgagaact cgttacgtgc tgggcgatat
     6421 gctcggtcaa tcaccacaga tggaacaagt acggcagact attttgctgt atgcccgctc
     6481 cagtgcagcg gtgttgattg agggggaaac ggggacgggc aaagagctgg cggcccaggc
     6541 gattcatcgg gaatattttg cccgccacga tgcgcgacag ggcaaaaagt cgcatccgtt
     6601 tgttgcagtc aactgcgggg cgattgccga atcgctgctg gaagcagaac tgtttggcta
     6661 tgaggaaggg gcgtttaccg gctcgcgacg cggcggtcgc gccgggctgt ttgaaatcgc
     6721 ccacggaggt acgctgtttc tcgatgagat tggcgaaatg ccgctgccgt tgcagacccg
     6781 gctgctgcgg gtgctggaag aaaaagaggt cacccgcgtc ggcgggcatc agcctgttcc
     6841 ggtggatgtg cgggtcatta gcgccactca ctgcaatctg gaagaagata tgcggcaagg
     6901 gcagtttcgc cgtgacctgt tttatcggct gagtattttg cgtctgcaat tgccaccact
     6961 gcgcgagcgg gtggcggata ttctgccact ggcggaaagc tttttgaaag tgtctctggc
     7021 ggcgctctcc gccccgtttt ctgccgcatt acgccaggga ttacaggcaa gcgaaaccgt
     7081 gctggtgcac tacgactggc cgggcaatat tcgtgaactg cgcaatatga tggagcgact
     7141 ggcgctattt ttaagtgtgg aaccgacgcc ggatttaacg ccgcaatttt tgcagctgct
     7201 actgccggaa ctggcgcgcg agtcggcgaa aactcccgct ccacgcttac tgacaccaca
     7261 acaggcactg gagaaattta atggcgataa aacagcagcg gcgaattatt taggcatcag
     7321 ccggacgacg ttctggcggc ggctgaaaag ctgaactgcc gaatgcagcg cgaacgcttt
     7381 atccggcata aatttcaata gatgatcacc gataaactta cgcatcgggc atgtttacat
     7441 agggttcaga attacttctt cttataaata tttgccgtgc cgtggatctt attgtcagtt
     7501 tgaccggagg tcagtaccaa cacatcagcc cctttttcat ccgctttttt gatcaaatct
     7561 tctttcgcgt ctgcagtcga catttcattg ctggttgaaa tatcacctat tttttcatac
     7621 tgcgattcaa ctttttcaaa ttccgctttg gtcatcaatt cagcggcata gacattggta
     7681 acggctaacg ctaacgcacc gactaacatt ttagagatta ttttcatatt acggctccag
     7741 agttgttatc acactatcac ccacccacat tattgggttg attaataatt agttcagtca
     7801 gggcataaac gccagtaacc ggaaataaga cactttgtga tctctctcgc acccgattat
     7861 ttttggtaga aatatgatga agttacttca cttatttatg gatgaaatca ctatggatcc
     7921 tttgcacgcc gtttacctga ccgtaggact gttcgtgatt acttttttta atccgggagc
     7981 caatctcttt gtggtagtac aaaccagcct ggcttccggt cgacgcgcag gggtgctgac
     8041 cgggctgggc gtggcgctgg gcgatgcatt ttattccggg ttgggtttgt ttggtcttgc
     8101 aacgctaatt acgcagtgtg aggagatttt ttcgcttatc agaatcgtcg gcggcgctta
     8161 tctcttatgg tttgcgtggt gcagcatgcg ccgccagtca acaccgcaaa tgagcacact
     8221 acaacaaccg attagcgccc cctggtatgt cttttttcgc cgtggattaa ttaccgatct
     8281 ctctaaccca caaaccgttt tattttttat tagtattttc tcagtaacat taaatgccga
     8341 aacaccaaca tgggcacgtt taatggcctg ggcggggatt gtgctcgcat cagttatctg
     8401 gcgagtcttt cttagtcagg cgttttcttt gcccgctgtg cgtcgtgctt atgggcgtat
     8461 gcaacgcgtt gccagtcggg ttattggtgc aattattggt gtattcgcgc tacgcctgat
     8521 ttacgaaggg gtgacgcagc ggtgagcaga taatttactc tctacgtaat caacctgatt
     8581 tgccgaatcc cggccatcgg cgatgatagc ccccgtaatg cgtggtcctt atttccgata
     8641 agtttacttg agtctttagc ttcccatttc agcccactct cccctgtgtg ggcttttttc
     8701 attggtctga cccgtcctga atagccaaac aatggcaatg ttccactttg cagggatgat
     8761 aataagggga tctgattcag aagagttttg aaaagttgga ccgccatcga ggcctcgaac
     8821 cccgtaccat acaacaatcc tggttgtcgt ctgctcttcc tgatgagcta atggcggttt
     8881 ttgatatgag ccttacagca tattttctgt caggcttgag tgcgatgcgt aagatacaga
     8941 aaagacccca cacaaacatg atggaattat gatttttttc atatgaatgt caacccacac
     9001 attcacaaaa catctgcatg agatcatttt ttatacaccg catggctgcg aaaccactac
     9061 tggtgctttt ttaagcgaag tgtcgccgat cacaacaaaa tatcggcgaa aaaagagatg
     9121 gaatgccagt tggcatacag atatacagat gaaatgatta cctgcattgc gattcactct
     9181 caggtggaat aacaacttgc aggtggaacc ctttctgaac taaagagtgt agcggatttt
     9241 aacgtgctta tttacaggat aatatctgtc acctaattcc tggactctat tagatatcca
     9301 ttgatttaat cgcgcatcat ctctgtatgt ttttacgtcc agaacagaga gcaataacat
     9361 caccagtgat tcaggaaaaa cagggtagat attttttaag tgtaataata atccatgcgc
     9421 aagcgcctgc ttaatgggcg cgtctgtctc cggaatagac gacatttcgc gaagccattt
     9481 ttccacatgc atacactctt tccctttagg gaaaattctc agcaaaggca tatggtgttc
     9541 aagaagaata catttgcaat ctgttcgcac tgaatcgata aactgcacta atttcttaag
     9601 cccggtaaag cgtccagcct gcacgtttgc tttaatatca agaaacgcat catcctggtc
     9661 atatacccaa actgtccctt tttgattttc aagtatgcga aaaacaaact taaccgggtg
     9721 atcagggtaa tccatccagt cataaaatcc agggaaatca ttaaactgca catttatggt
     9781 gagtgcgtta tcgtctgacc actcacagtc tcgtacggca gccatgagat gaatgagttt
     9841 gtcgtcatcg actaaatcat cacgacaatc ataaggataa tcataatatt gtttaatcaa
     9901 aaccttgaat atatcgacac aggaaaccag ggagcttttg atatcctctg gtaactgatt
     9961 gtttttaacc gtattaagta tgctttgttg cgtatatggc atcaaattat tgtgcggagc
    10021 ttttaatgat atcatcgcga tctctcttaa ataaaaataa acgtcgcaga taataaagct
    10081 ctgattaaac caggcactat cagaaatcgc tcataactaa cgtgtgaagt attgtgtact
    10141 ggagggagtt aatttaataa tttgaattta cgttataggt tttactgcta ctttgatatt
    10201 atttcgctct ccttaactaa aaagttaagt ggagagcgaa gttaatatgt taaaccacag
    10261 cgtatttatt aattttttca gtctgttagt gtgcgattat cgataacaaa acgatatttc
    10321 acatcaccgc gcagcattcg ctcataggct tcattaattt gatcggcccg aatcatctct
    10381 atatcagcca caatgccatg ttcggcgcaa aaatcgagca tctcctgagt ttctggaatg
    10441 ccgccaatca tagaaccggc tatcgcacgg cgtttcatga tcaggttgaa aacttccggc
    10501 gatttatgcg gtgtcgcagg cgcaccaacc agcgtcatgg tgccatcacg cttcagcaag
    10561 gtggtaaaat cgtcgagatt atgtggcgca gctactgtat tcaaaatgaa atcgaaactc
    10621 ttcagatgag ccgccatctc atcggcattg cgtgagttaa caacttcatc ggcccccagg
    10681 gcttttgccg cttcgcgttt tgcctcagaa gtggtaaatg ccaccacatg tgcccccatc
    10741 gcgtgggcca gcttaatccc catatgtccc agaccgccga tgccgaccac gcccactttt
    10801 ttacccggcc cggcctgcca gtgacgtagc ggcgaatacg tggtgatccc tgcacacaac
    10861 aaaggagcca ccgccgccag ctgctcttgc gggtgacgaa tacgcagaac atatcgctca
    10921 tgaacgacga tctgttgtga gtagccgccc agagtatggc ccggttcgtc cggcgtcggc
    10981 gagttatagg tgccggtcat gtgatcacag tagttttcca acccgtcttc acactcttcg
    11041 caatgtttac aactgtcgac aatgcagccg acaccgacca gatcgcccgg cgcatatttt
    11101 tctacctgat caccaacggc taccacacgc cccacaattt catgacccgg cacgcagggg
    11161 taaaccgtcc ccgcccactc ggaacggacc tggtggagat cggaatggca aacgccacag
    11221 taagcgattt cgattttgac atcattcggt cccggttcac gccgggtgat atccatcggt
    11281 tcaagtggtt gtttagcgga atatgcacca acagctttga tcttcattgt gtttactcct
    11341 gattagctat gtgtatttgg cgacgcctga tatgtcatcg cggcaaaacg cgtccattga
    11401 atatagccaa tatttaaacc acccgatcca gccccaacat gccgcgagcc tgaatgaaac
    11461 agagcagcaa cgttccccat aaggccatcg tcaggtaggg gcttataccg agaatattga
    11521 agccgctttc aataatttgc agaagaaaca gcgccagcac cataccaatt atccgcccgc
    11581 tgccgccgtc cggattaacc ccgccgagta ctgccgcgag gattgacacc agcaagtacg
    11641 attcgccata agaggcctta gccgagttga gtttcgacat catcaaaaat gccgctaccg
    11701 cacaaagcaa ggcggaaatg acataaaccc aaatcagcac ccgacgagtg ttaatgccgg
    11761 agtaaagtgt tgctcgttcg ttagacccaa tcaggtaaat ggctttaccg agcggcgttt
    11821 tctccagcaa tatccatagc cccagtgcca ctgcggcaaa caaccacatc ggtagcggaa
    11881 taccgaacca ttgcgcatga ttcaaccaca gcacccatga gggataattg gcaatggcgc
    11941 taccacctgt caccagaata tttatccctt tgagcaacgt catcatccca agcgttgcaa
    12001 gaattggcga cactcgaata ccagcaatca atacgccgtt acataaaccg attatcaccg
    12061 ccgcgccagc gcccgccagt aacgtagcga caaccgtagc aatacccggt ggatactgag
    12121 tcgccaccca cgccatcacc agtgaacagg cgttagcagt ggctataatt gacaagttaa
    12181 tgccaccgca aagcatggtc accgccatag ccaacgccag aatgcccagc acgggcattt
    12241 gcgaagcgac ggactggaaa ttacttatcg accagaaaat ctccggcata gtaatgctga
    12301 acgcggcaac agttagcgcc agcaggccga tcaagtaaaa ctcaacgtta ttacgccacg
    12361 attttttcat agcagactcc ggttctgatg ctgactccac gccgtggcac taatgctggc
    12421 aacgatgatg atgccggtga tcaatgtttg ccagtaagac gagactccca ataaatttag
    12481 cccgttttgc atcactgcca acaacaccac gccgagcaaa gtaccggtta acgtgccgcg
    12541 cccgccgagc aaactagtgc cgccaagcac caccgcagcc agtactgtca gctcataacc
    12601 cagaagagaa tcgggggcga cagtcatcac cgtccacgac tgcactacac ccgcagcgcc
    12661 agacatcaat cccatatagc catagacgaa aagttgcagt ttcagcacgc taaagccgat
    12721 gcgactggct gattcgcggt tcccgccaag ggcataaatt ttgcgcccta cactggtgaa
    12781 attcatgata aacgctgtta gcagcaccac cgtaatcatg gcgatcagcg gcagaccaag
    12841 gccatagtca tagccatcag catcggtgta cttgaacaac ataaccccct gctcaaacca
    12901 cggcggaaag tcgtacagcc acacaccttt actcaaccat aacagcaggc catagaaaat
    12961 attcagcgtc gagatagtga tgatgataga aggcacccgt aggcgattaa ccagtaaggc
    13021 gttaattaag ccaagtaaaa tgccgatgca gcccgccagt gcaaacgcga cagcgaagtt
    13081 gccaccaatt ttttgcagca acagcaccat gccgtattgc gcaatgatgg tcattgctgg
    13141 aaacgaaata tcaattccac cggaaatcaa caccacaaac aaaccacagg cgagaatagt
    13201 caacatggca tagttattgg cgagatcgta caaattaccg aatgtcagaa actcgtcgct
    13261 gcgccaggca aggccaacaa acagcaccac aatcagtaat gccagccaga attcatggcg
    13321 ttttttcagt tcagccattt accacctccg caatcctggc ctgagaactg ctgtcaggca
    13381 ggaagctatg ggtgatctga cctttttgca ttaccaaaat ccggtggctt tgataccacg
    13441 cttcttcaat ttcatcgcag atcatcaata ccgcaatgcc atgagccgcc agatcgctga
    13501 taatgccgta tattcctgct ttgttggcga tatcaacacc gactgtcggg ctatcgagaa
    13561 tcaacagtcg tggcccaatc gccagccatt tggcgatcga tactcgctgc gcgttaccac
    13621 cagaaagcgt attcaccgga agatgaggat cagagacttt gatggtcaat gccttgatta
    13681 gcctgtccac caggtcacga actttagttt cactcagaaa gccgaagcga tcttttacct
    13741 tgtgaaaaac agtgctaatg atgttgtctt caatagattg cgccatcacc aaaccgcgcg
    13801 acatgcgatc ttccgaaaca taaccaatac cgtggcggat agcgtcctgg ttactatgca
    13861 gtgtaaccgg ctggccgttg atgaggatct cgccagcatc cggtcgggtc ataccaaaca
    13921 gactcagaca taattccgtg cgccccgcac caagcaggcc aacaatcgat accacttcgc
    13981 ccgcttccac ccgcaagtta atatttagat attccccatg acgacttaaa ttacgcacct
    14041 ccagcaccgg ggtgctggat tttccctgcc acaactctcg cacctgatat tcaaactcct
    14101 ggccggtcat caggaaaccg agttgttttg tggtcatctc tgcagccgga aaagtcccga
    14161 ccagctcacc atctttcagc actgaaatac ggtcggaaac ttccatcact tcttccagac
    14221 gatgactgac gaagaccacg cagatcccgc gttcacgcaa ttgatgcacc acctgtaata
    14281 acccttgcac ttcctgatgg gtcagcgaag cggttggttc atccatcacg atcaaccgtg
    14341 cgtcctgcgc cagtgcacgg caaatagcca ccagctgaca gcgggcaatt gaaagctcag
    14401 aaacgagtgt atccagtggc aatgtgacgt taatactggt cattgccgcc tgcgccacct
    14461 cacgcagacg acggcggtta acaaacagac cgtggtgata gtgattcacg gcgatattct
    14521 cccagacact caggttagga aataaagaga ggtcctgata aatcacctga atcccctgcg
    14581 ccaccgaagc ggcgggtgtc agctttgacc agtttttccc accaagcgta atttccgctc
    14641 cctcatccgg gcgataaaca ccggacataa ttttaatcaa cgtcgattta ccacagccgt
    14701 tttgcccggc aaggcagtgc acttcacccg acataaagtc gagagaaaca tcgcgcagcg
    14761 ctcgcgtggc atgaaacgtt ttgttgatat gacgaaggga aaggaaagtt tccataacat
    14821 cccctggcaa taagaatcgg ggctttcgcc ccgaagaaat ggattaataa agtgagtcga
    14881 tattgtcttt gttgaccagt aataccttat ggaaacggat aattcgctta tccatatcta
    14941 cgtcggcctt acccagattc tgcatttcta atccaggttt gatctcttca cccttcagca
    15001 acgtgctggc gacggctgcg agtgcgtaac ctgcagaggc cggatcgtaa gtaatccctt
    15061 cggtgatatc accactttta atcagtgatg ccgcctgtga agggatcatc atgccataga
    15121 ctgcgacttt atttttcgcc cgtttctctt tcaccgcacg tcccgcgcca atcggaccgt
    15181 ttgaaccaaa ggagacaacc gctttcaagt caggataggt tttcatcagg tccagtgtgg
    15241 tacgacgtga gtcatccaca ctctcggcaa ccggcatgcg gcgggtaact tcatgcatat
    15301 ccgggtaatg ctctttctgg tatttcacca gcaagtcagc ccataagtta tgctgcggca
    15361 cggtcaaact acccacgtaa atcacatagc cgcccttgcc ccccatgcgt ttcgccatat
    15421 gctcaacata ttcagcggca aatttttcgt tatcaatgat ttcaatatcc cagttagcac
    15481 ttggctgacc gggggattcg ttggtcagaa ccacaattcc ggcatctcgc gcttttttga
    15541 ataccggttc cagcacgttg gcatcgtttg gcacgatagt aattgcatta accttacggg
    15601 cgattaaatc ctcaataatt ttaacttgtt gcggagcatc agtacttgaa ggccccacct
    15661 gtgaggcatt aacaccaaag gctttacccg cctcaaccac accttcgccc atgcgattaa
    15721 accacggcat accatcgact ttagaaatat tcaccacgac tttttccgct gcctggagcg
    15781 gcgcagaaat tagcgcagcg cctaataaca gcgaagacac catattgata acaaaacgtt
    15841 tattcatcat ttcagcctca ttgcagggta gtcaggaagt gttcgcccaa attagcgcgc
    15901 tggagagaat aagaattttc gaatattttt atgcagaata aacagcccta tttcaggcag
    15961 ctattaaaaa tatggttata aatatggaca aaacgggcag caacaataag tgtgacattc
    16021 atcacgtaaa aaaatagcaa aataaacaaa attagcaata ttttatgtgt gtgagtttat
    16081 ttcgcgtaga taaatagtta tctacacaac gtcaataagg gcatattctt caaatataac
    16141 gtccatttct ggtgaatagc ttccttctgc ctggaaaaaa cgacggtgtt cattacgcca
    16201 atattcaaga ctgaggtcac cttcaccttc cttacgggca agctccacgg tgacatcaga
    16261 aaagcgcagt aaatgcaggc caatcactct gataacacat acaggctcac cacggctatt
    16321 ttctacaatc ttatactcac caatcatcgg aaatgcgttg tcttcaatac atcccgccag
    16381 cgaaccacag gaagctgttt taacaccagt cgcaatcaac gttgcgaggt gatcggccaa
    16441 cgctgctgaa tcgccaaaag tacaaaagga agcatgggga tatttttgtg agaatttttc
    16501 ttttatggtc atggcaatat ttttgcgatt acggaaaaac aaatcacatc ttaattactg
    16561 ttggtaacca tcagcaagag tatcattggt ttttcgatac tgaatctttt ttatccgaag
    16621 caggtcacat ttatggcctg ataaatacct ttgttaaaac tcacttttgt gtatttctct
    16681 caaataatcc cctggaaatt atgccgcgag attataaagt tcaggatcat tcattagcaa
    16741 accattcccg ccccactccc tggggcttaa atctcttaca attcaccgtt gcaggcgttt
    16801 caaatgcgca tcaacctgcc acactccccc acaccagttg ccctttatgg aacgttgcgg
    16861 ttctcggcga gatacgcgcc accgcctcgg cggaacagga ggcgtccacc agcacaaagc
    16921 tggcatcatc ctgcgctttt ggccatacac gctcgccttt ttcattcagc ggcaatacat
    16981 cgccagtggc tagaaacagc gaacgggaga ggttctgttc gttaggacga atatagagct
    17041 gcgcgtagag attggctttt tccagcatgt cgcccagacc ataaggcgac cagtggtcga
    17101 taacgctgtc agtgccagtc atcactttta cgcctttgtc gtgcaactgt ttgagcggca
    17161 tatgcagcgt gccaatcggc accgtcgagg cgatagaaat ttgctgcgcc gccatccgat
    17221 tcgccagcgc atcgacctgt tgttcgttga gcgtagccag cgcaaaggcg tggctgatgg
    17281 tcagcttacc tttcagttgc ggcgttttct ctaccgtttc aaccatataa ttgatggctg
    17341 tcacgcccga cggagtggtt tcgtgcaggt gaatatcgac acctttgtcg tagtccagcg
    17401 caatctgaaa catggtgtcg agggattttt ccatcgcgcc atcgacactg gtcgggtcca
    17461 gcccgccgac gtaatgcgca cccgcctgca tcgcttcgcg cattaagggt tccgatttcg
    17521 acagcagcaa accgtgctgc gggaaggcga cgatttcaca ctcgaagccc gcctgacgtc
    17581 gcgccagcac cgcctgcaaa ttctgcagat ttttcaggcc ggaaaccggt tcgatattgc
    17641 agtggctgcg ggcaatggtg gtgcctttcg actgcaatag atcgatcagt ttttctgccc
    17701 gttcctgcgt gtacggttgc agttccggca gcattttctg ctcaagcttg atcatgtcct
    17761 ggatagtggt gcctgccgga cgattgagcg agcgccacgg cccgccgtag aaggttttgt
    17821 cgagatgaat atgcatgtcg cgagtggtgg gtagcatcaa cttaccgcca gcgtcatagt
    17881 gcggcagcgt ggcgtccgga tgctgcttgt tctcgcgcag ggcgacaatt ttgccgtcct
    17941 gaatctccac ggtctggcgc gccgtgcggg tctgcaccgc cacaccgttt tcgtagtcaa
    18001 aaccggtttc cagcagcacg ttatcgagat agtaatgcgg gtcagtgatt ttcatggtgt
    18061 tgttcgcttc gcagtttagg gtgccagcta ccgccgcatg ggcgagcggc acagaggtac
    18121 caaacagcgc ggcggcggtg accatcttac cgctctggct cagaaattca cggcggctat
    18181 tgctttcttt catcctacgt ccttacgttt taataatatg ggtgcccgtt tcaccgcgca
    18241 gcgccgcagg caggcattcc ggcgtggtga taatcacttc tttgccgccc tgctccagga
    18301 acgtcaggct ggcgatgatt tttggcaaca tgctgccggg cggaaaatgc ccttcctgca
    18361 tatagcgagt catggtggca atatccaccc gatccagcgc ctgctgctgc ggtttaccaa
    18421 agtggataca gactttttcg acgccggtgg tgatcaccag aatgtcggcg tgaatttcac
    18481 gggccagcag cgcggtagag agatctttgt caataaccgc gtccacgctt tggtaatccc
    18541 ccgcttcagt acgcactacc ggaattccac cgccgcccgc gccaattacg acaaaacctt
    18601 gttggatcag cgccttaatg gcgggtgctt cgacaatacg tttcggttcc ggcgaggcga
    18661 ccacgcggcg atagccccgt ccggcatctt caacaaaaca ccagtcaggg tttgcctttt
    18721 gtagtttgtc acgctggctt tcactgaaga atgcaccaat gggcttggtg ggatgggcaa
    18781 aacctggatc gtttttatcc acttccacct gagttaccac ggtgacggct tttttctcgc
    18841 cgtgacgcgc cagccggtta ttcagcgcct gttggatcag atagccgatg ccgccctgcg
    18901 tatctgccac acagttcgcc agcggcgtta agggcagccc ttcgcgctcg tgggcaatct
    18961 ccgcacggcg taaatccagc ccgacctgcg gcccgttacc gtgggtcagc acaatgtcgt
    19021 aatcggaagc cagcatttcc agcaccgtat cggccacggc tttcaccgcc tccgcctgat
    19081 gctcaatcga ctggctggcg ttatctttga taatgctgtt gccaccaatg gcaacgacca
    19141 caagctcttt catgttgtta tgtccgctac agagggtgat taggttatga ccaacgccag
    19201 actgcctgat gcgctacgct tatcaggcct acaattctcg ggccatccgt aggccggata
    19261 aggcgttcac accgcatccg gcataaacat gtaagtcaga cgccgtaacg ttcgcaccag
    19321 ccgagaatgg ctttctcgaa gcaggcgaat ggtggatgca caatgcccgc gccaatcatg
    19381 ccgacgcccg catctttatg ggcgatggcg gtgttgatca ccggcagaat gccgctgcta
    19441 cccacgcggg tgatgtcgat tgccgacggc acgcccataa aaccgagcag cggaatggtg
    19501 acgttggggt tttcgccaag ggtgatttcg cgcatctgac gggagaaatc gatagcctct
    19561 tccaccgtgc cgcccaccag tgcgacgatt gcaggcgcgg tcgccatggc aaacccgcca
    19621 ataccgtagg tttctgtgat ggcgctgtcg ccgatatcca gccccgaatc ttccggctta
    19681 taaccagcaa acatcgggcc gatgacctgc tgcgctgggc cggtaaacca ctgccccggc
    19741 aggccgctga cccgcaggcc gaactcgacg ccgttacgcg ccatggtggt gaccacggtg
    19801 ctgtattcga tgccgtgcgc cgcatccatc gccgctttac acatcgccat ccacgtcggg
    19861 ccggagaagt agtcgctgct ggcgacaaac tcgaacactt cgcgctgttg ctcgacggaa
    19921 tagcctgcct gaatgatccc cggcgtcagt gcctgaatca gcagcgtcgt cccggcgtta
    19981 ttgcggttat ggcactcatc gcccatatgc agcgcctgcg ccagcattaa gcgcagatcg
    20041 atctcaccga taattttcat cgcgtcgcgc agcattggcc cctgcacatc gcgcatccag
    20101 ttgaggcggt cgatcacgct ctgatcgttg gcacccatac gcaaaatctt cgccatctgc
    20161 tcgctcatat tggtataagc gatgttgccg taggttttgt ttttcacgat gtgcataaac
    20221 atcgaggccg aggtaacgcc cgccatcgat cccacgcagt cgtgttcgtg gcacggcgag
    20281 aaggtgatct ccccggaggc cgccagttca gtcgcctcat cgagatcttt cgccagccct
    20341 tcgaacacca acgccccggt gaccgcgcct ttcatcgcgc cgcacatttt ttcccaggtg
    20401 atcggcggcc cggcgtgaag aatggttttc ggcgtcatgc ccggcaccac gttaatcgcc
    20461 tgatcaaaac caatcagcac cggatgcgac tggataatgc gctccagcgc ctgctggtta
    20521 gcggcggcga ttttgtctgc cagcggcgaa tcggcaatgt tatccagcgc ctgcaccacc
    20581 tgcatattgc cctgccccgg cggcgtccag tcgagttgag tcacttctac atgctgcttt
    20641 ttcaggtcat cgctaaacat ggcgataccg acgttaataa cgttcaacgg ttggctaaac
    20701 agtgactggc tcatcaggct tcctcccctt tgcagataaa ttcacgcgcc agcaatccgg
    20761 tattggtgct gctgctcgcc agaatcactc cggcatcaag cagcatctgg ctttgttgtt
    20821 ctaatgatgg cgtatcgaga tcggtgccca gcacataggc gagaatgatc aactcgcgtc
    20881 cttcggcggc ggcgatcgct ttcgcttctt tgatcgcctc gatggtggag ccgaccggat
    20941 cttcatgcga tccaaatccg agcacaaaat ccatcacgat caccgccact tctggatcgc
    21001 gcgcctcttc gatcaagcga ctgatgcggt tggtggggtc aatcattgga tgcggcttgc
    21061 cgttggtgaa gtcgtcatcg ccaaagtcga ggaaggtgtg tttgatgcta cggttgatat
    21121 cttgcaggcg gaattccgga tcgggctgaa tgttgctgta gacatcgccg tgtttttcca
    21181 tcaccgcgaa catggtttcg tcacacagcg tgccgccaca gaacaggcca cgaatgtact
    21241 tctgctgcgg ttgcaggcga gcacgtacgt cagcaatcaa cggctggttc agcgtatgca
    21301 ggtcgagatg ttcctgtttc acgccggaga gcatcaccgc tttcaatgcc gcctctttgc
    21361 tgccgcgggc aaactgtagc ccctgctcat ccactggcgt ttcgccacga ccgaggaaac
    21421 agaccaccac cggcttgcgg caggcgcgcg cgcgttccag cactttgcgg gccaccgcag
    21481 gcgcgggcgg tttggagata agcacaatga tttcagtttg cggatcgttt tccagcatgc
    21541 cgatggcgtc gagcatcatc aggccgccga ttttctcgct caggtcgcgc ccgccggtgc
    21601 caatcagttg cgaaacgccg ccgccaaatt catgaatgcg gacgctcagc tcctggctgc
    21661 cggtgccgga tgcgccaaca ataccgatgt tgccgcgacg cacggcgtta ccaaaacaga
    21721 gcgccgcgcc gttaataatc gccgtgccac agtctggccc catcatcagc agcccttttt
    21781 cgtgggccag ttgcttgagc gccaattcat cttcaactga gacgttatcg gaaaacagca
    21841 tcacgttgag atcgttttgc agcgcctgac gcgcttcgcg agcggcaaac agaccgttga
    21901 ccgaaatcac cgccaggtta ctttccggga tatgcttttt ggcgctggca atagtggcgt
    21961 aacgcgcctc atgcgagccg ctttgcgctt tggtgttgaa cagttcttca atctccacca
    22021 gcaactgttc gttgtccgcg cccgctttgc cgttgatgac aatcatcagg tcgccgtttt
    22081 tcgcctgttc cagctccggc gtcagcagtc ctaaattttt cagtacacct ttgttcattt
    22141 cggtcgccat cgccacaaat gcctgctcga cgccgtcgag tttatttgca cgcgtggaga
    22201 tagacatcag cgagacagaa tcaaaatagg tattcggttt aatgactatt ttaactgaca
    22261 taattccctc cgtaggtttg gctcagcgca caaccatcgg ccatgccata cagcatgtcg
    22321 cagccggaac tggagccaat atgtttaatt ttttctattg cctgagttgc gttcccaggg
    22381 atgtcataaa taatgttgtg aataaaacga tgaatatttt cccggcaacg ttgttgtaat
    22441 gcggcttcca gcgttatagc acttaataat gtggtgttat ttctgccgcg ctgcataccg
    22501 agataaaatt cctctttgta tttttccgac ggatgcccgg gaataaataa aataagcgcc
    22561 agacctgtta aataatcgtc ggctgatggc gtaaggccaa tccctaaccc aatcatactg
    22621 gcgaccgctg cggcgatatt ttgtttttcg tttactgcct gaataactgc ccgtcgttta
    22681 atatgtagtt ctttatttaa cgcctgataa aacggattat cgccgcgata attaaacagg
    22741 gtttcatttt cccccaggtg ctgatgaata atgtcgtgcc actgttgcca ggcgataagg
    22801 tgaaaattca cagcggtcat ttgcaaggtt ggggacagcc aacgccgaca acgactcatc
    22861 tctatatgaa gatgttgacc aaccgtaata ccttgatcgt caaactgaac cttatcacca
    22921 tgccggaaca gatcatcaaa gtgagtgagt gccaaccgac aactgtttgg cgcattgtcg
    22981 tactcttcac aaagcagcgt cagcaactgc tggcgagccg ggagtgcgat atttaccgct
    23041 ctggcaaaga cctgttcaac ctgtccttgc ccccgctgcg ccagaaaatc cgcatccgca
    23101 gttagcgccc acacacaccg tctttctggt gtgttgatag caagcctccc ggtttatgca
    23161 cctgcggcaa tcagtaactg cgcaatctct tcaaagcccc gttcccgcgc cagttccagt
    23221 ggcgttttgc catatttatc ggtcagatgc gggctggcac cgtgttccag caataactgc
    23281 acaatcgcct gctgtttaat accaccatca ttaagcacaa tcgcttccag tagcggcgtc
    23341 cagcctacat ggttggtctg gttaacgtta atctccgtat gcgccaaaag ctcttttaca
    23401 atacttaaat ggcctttttc acaggcaggc gtcaggccga caccgccaaa acgggttacg
    23461 caattaagat cgggtttagc cggtaaaata attcgtagta gcgttaaatc atcgttcaga
    23521 cagctaatta aaaaaggatt taaacaggta tgatcttgct tattaatatc cgctccggca
    23581 tcaattaatg cctgaacgca agcatattgc tgatataaac ttgccagcgt aattgccgtt
    23641 ttcccctgac gatcgcaggt attaatatcg acacccagcg caaggcaggt ttttacttta
    23701 tcgatatcac cctgttgtgc agccaataaa tagtcggcag gtagattttt tatactcatt
    23761 cacattcccc tggtttttgt ggctgttttc gatgaattga gaatagcaac gataatctga
    23821 taaaagaacc cgcttaaatt taattcgctg tatttcgcct ttttgttgaa tgatgttcaa
    23881 caatcttttc gcagtgtgcg gtagtgcaat atttgtttct ttgacccgat accctttcac
    23941 tcaatcaggt tttatttctc tatagtctgc acatgtaaga gtagctttcg tccatgtcta
    24001 accagagggg aacaagatga acggactcac ggcaacgggt gtcaccgtcg gtatttgtgc
    24061 agggctttgg cagttagtgt cttcacatgt cgggctctct cagggatggg aattattagg
    24121 aaccattggc ttcgtcgcct tttgcagttt ttatgccgca ggcggcagta aatcggggtt
    24181 tatcagaagt ctggcggtta attattccgg gatggtgtgg gcatttttcg ccgcgctggc
    24241 tgccggttgg ctggcctctg taagtggact gtccgcgttc tgggcgagcg taattactac
    24301 cgtacccttc tcggcggtag tggtctggca agggcgtttc tggttactgt cctttattcc
    24361 cggtggcttt ctcggcatga cgctgttttt tgccagcggg atgaactgga cggtgacgtt
    24421 actgggtttt ctggcgggta actgcgtggg gataatttca gaatatggtg gacaaaaatt
    24481 aagcgaagcg acgactaaac gcgacgggta ttaaaagtta ttcaaaaatg attatctatc
    24541 tttaaccgat acctctacag tcatagctgg agaaattcat agtagataat ggcgagaatg
    24601 gtttaatgaa ctcaattttt accgaagaga atctgttggc atttactacc gctgcacgtt
    24661 ttggcagctt cagtaaagcc gcagaagagt tgggtttaac cacttccgcc attagctaca
    24721 ccatcaagcg tatggagacg gggctggatg tggtgctgtt cactcgcagt acccgcagca
    24781 ttgagttaac ggagtccggg cgttattttt tccgcaaagc taccgacctg ctgaatgatt
    24841 tttatgccat caaacgcagt atcgatacta tttcgcaggg catagaagcg cgtgtgcgca
    24901 tttgtattaa tcagctgctt tatacgccca aacacaccgc acgcttgtta caggtgctga
    24961 aaaagcagtt tcctacctgc cagattaccg tcaccaccga agtgtataac ggcgtctggg
    25021 atgcgattat taataatcag gccaatatcg ccatcggcgc acccgataca ctactggacg
    25081 gcggcggtat tgattacact gaaattggcg cgattcgctg ggcttttgct atcgcgccgg
    25141 atcacccgtt agcctttgtg ccggaaccca tcgccgaaag ccaactgcgt ctctacccta
    25201 atattatggt ggaggacacc gcgcatacga ttaataaaaa agtgggctgg ttgctgcacg
    25261 ggcaggagtc gattctggtg ccagatttca acaccaaatg tcagtgccag attttaggtg
    25321 aaggaattgg ttttttgccg gattacatgg tccgtgaggc gatgacgcaa tccctgctgg
    25381 ttacccgaca aatccataat ccgcgccagg attcgcgcat gttactggcg actcagcatt
    25441 ctgctacggg tcaggtgacg cagtggatta aaaaacaatt tgcgccaaat ggcatattaa
    25501 cggggatcta tcaggattta ctgcatcggg agaattaatc gctgccaacc tcccgatgcg
    25561 ctgtgaatag cggtttacat caaccacctg ctttcattac ccattcagag ataaatttat
    25621 tacccggtac tggcggagag tacaaatagc cctgcaaaaa gtgaacgcct ttaccgatca
    25681 ttaaatccgc ctgctcctgg gtttctaccc cttccgccac gatactcaga ccaggcttac
    25741 gcgctagttc gacaatattg tccacaatat gaccggagat ttcgtcgaca ctcgccattt
    25801 gcacaaatga cttatcgatc ttaataaaat cgaccgggaa cgcctgcaag taacgatagg
    25861 tcgcataacc cgtaccaaag tcatccagcg caaaggtaat gttgtgctga tgaaggctgt
    25921 caaatatcgc tctggcttct ggcgttaccg gaatagggtt acgttccgtt agctcgagaa
    25981 ccagcttgat tttatcgtta cctaatttct taaccaggtt cagacactct ttttcaaatc
    26041 ccgctgccaa aaaacaaccc gccgagacgt tgatgccaat atggaaattg tccggcagca
    26101 aatgttttac cggcatcaga atatccgcag tctgtttcat cagttggcgg gtcattatga
    26161 caataagacc ggatgactcc gccagaggaa taaactgatc cggtgggata attcccgttt
    26221 gtggatgttc ccagcggaca agtacctcac agcccgtcag taccccagtc tgcgcgcaga
    26281 aaaccggttg gatccacggt ttgaattcgt ggttttccaa cgccagactg atggcttcag
    26341 gcgtgacgat atggtgacaa cggttatcag ggatataaaa attattttta tttcccgtgc
    26401 cactgatgac ttgcggatga tattgaaaaa agatatcccg ccagaaggta atatcactct
    26461 ggatgcctag tttttcatac agctgcttct tttgatgtga aattgtcttt gcactgcgat
    26521 ttctgtactg tgagatttct gtaaccgaca ttccctccat tagcagcttg agtaaaagcc
    26581 gttctttttc ggacacgata ccaggcaacg tcaaattcac cgttgccccg aactcttcca
    26641 aaaaaacact caaatcatgc gaagccattg aacacacctt gattaaatct tgtgcgccag
    26701 ctaaatctgc gagtcttcac tctgactgat aaagctggct gcaagaaaat gacacagaga
    26761 gccgaattat ctcccgttcg gctctctctc cttttactca ttattcagat cagaattgcc
    26821 acttaatgcc aaccattgca gaggtgtcat tatatcccct atcggcaacc tgaacgccca
    26881 cattccccca gacattcagg ttggcattta gctgtccttc caccccggtt tttatctcag
    26941 caatatttcg ggctccatcc tgagtgacgg acacgccatc catactggtg ctgaaatcct
    27001 tactgttatg cagccagttc acttctacaa acggctggaa ctcgcgggat ttaccgtcat
    27061 ccattttatg gtggctcttc agccaggttt ttacgccaag tcgggtttga acattgccat
    27121 caccgttgct atgaacgagg gttccgttgc tttcgcggtg cttatcggct ttgactccca
    27181 tccaggtaac ctgtgcttgc ggctgaacat accattcatt acgcgttccc tggttgccat
    27241 taaattcagc taatttgtgt ttgtatccag cttccagtga agcggtaaat ccttttgatt
    27301 tataggattc actttgtaag tcatcccctt tcactgtgtt atcaaaccag ctgtactgcg
    27361 cccaactgtc gagatacgcg ccattacgcg actcgtcatc ggcataccag gtggcataga
    27421 ggccggtgct atatccgttc acactcgctt ttgcacgata accggttcgc gaggaaatgg
    27481 ttttgctgtc gctgttgcca tatcccgcca tgaccccaac atgccagctg tcgctgccgt
    27541 tttggctcca ctgcgcgaca tcgcctccca gttgcagaac atagcgattg ctttgggttt
    27601 tcagctggcc gctgccatca cgccatttat tatgaccacc ttcatggcgc atccacatag
    27661 tggtttgttt ctgctcaccc gtcaccatgt cggtatagta cgtattaccc agacgctcat
    27721 gcagacgcgt ggtgaacatt gtattcgctg ctgcaaggtt cgcaatgtag ctacccgcct
    27781 ccggtcgcag gtcattatcc acattcagat ccggacccgg cgtaggtgtc gggttagggt
    27841 tcggctctgg attcgggttt ggctccggat tcggcatcgg gtctggctcc ggctgcagtt
    27901 caggagaatc actgccgctg gtcagatacc agttaccacg atttgcccct tgtccacgcg
    27961 cgagagtgta gtcatacgcc cccgcaacga tacgaccagc ctgaacaaat tcgccctcag
    28021 acttaccgtc tacatggata agttcgatac cgttaagggt tttcgcacct gtaccgccag
    28081 cgttattcac cgtaacggca gtcgtgccgc tagtatcgcc ctcgacaacc aatttatcgg
    28141 ttactgagtc atcgccattc agtaccgtat tcattactag ttgaccgtta ttaccgtggt
    28201 agttcccctt caccactaac acattgccag cttgctgtcc tgacattccg gtcatcagcg
    28261 taccgttgtt ggtcagattg ccgcccaggg tgtaattcac agacgcagcc gctctttttt
    28321 cagcgctatt attcaacgcc agggtaccgt tgttctcaac gtttgcgcta cccaatccct
    28381 gcgcatgact taccaggaca ttagccgaat tagcaatgtt cacattcgcc gtcaggttgc
    28441 tgttatcgcc cgcgatgtcc agcgtgccgc cctgaatatt cagctctccg ctccccgtta
    28501 acgaaccggt tgaagtgccc ccgttggtaa ccgtcagact gccgccattt aagctcagca
    28561 gtgaatcggc gctgccattg agcgtgccca cggtctggct gtgaccattc atatccagtt
    28621 cagtctctgc cgccagacgc agttcatggg tattacccaa cacgttatcg ttattcaaca
    28681 aaagcgtccc actgcgcaga tcggtaacac cggtatagtc gttgtctttg ttagaaagcg
    28741 atacggtctg atccttctgg ctgctgaatg ccaggtcacc gctgccggta atctttgcgc
    28801 tgagatcggc ggcattctcg cttttaccgt tagagctcag caccagagca tcgctatcgg
    28861 tagcgtgtaa atcaagctgg gtcaggccgt aaccgatata cagaccgtca ttgtttaccc
    28921 cactcgtcag acgatagtcg taagtgccga cggccgcttt ttgcccattc tgctgaatat
    28981 cacgttgggc actgctggag aggatttgcc catttttatc ctgcaattgc agattgcccg
    29041 catcgcccag aacttccgca ccttgcgcat caaccaactt aatggtgctg ttaccatcat
    29101 cgacttcagt gagcgataac gcagagtcaa tatcacgaga tactgagctt acaacgtcac
    29161 tgtcagaaac ctgaatcaca ccctcgccgc gcaaatccag cgttttacta acgttgaccg
    29221 tcccttctgt catctgtgca cctgctgtca gggcaccaaa atcaacggtt ccgccggaga
    29281 atcccaaagt gctaatggag tgagcgccct tacccactgt taccaggctt ccctgacccg
    29341 cagtgatccc gcctctctgc agaaactcag cattcttttc caggttaaat gtcgtgttct
    29401 ggaacgccag tgaaccctgg aaccagtctg tataagcaaa ctcatcgaag gagaattcgt
    29461 gattccccat gtttatttca acaatacctc taccttgaag atcgttatca aatacccaat
    29521 ctttattggc attgatttgc agtttgccat caacatccag gtccgcacgg ccgatgttct
    29581 tgtcttcaga aataaccgcg acagcaccag aagcgacatc aatatatccg ctgaatgcat
    29641 agttattgcc ggtcagtgcc actgtcccac tgtcgatact tattgtacca ttgccggaaa
    29701 tcttattggc aaaggtcatg ctatcgtcag ccttcaggtg taatttcccg cggttgttga
    29761 tctctgctgt acctatattt ttggtgatcc ctaaagcgcc attttgctca attttgactt
    29821 taccactaaa ggcagagtta tcaccggtta agtaagtcgt gccattgagc gcattgatat
    29881 agcccaggcc ctcgctgcca ctgacaatat ccgcggaaat agtcgcgttt ttatccgtgt
    29941 ggtttaaggt gattttctta tcggatgtct ttgcccacac aaactcaatt ttttcggcat
    30001 caataatccc tgcagccttc gcagcacttc cttcctgtgc acctaacgct aactcagagt
    30061 tggtgcttaa actaattttc ggtgcggata ttttacctcc atctgatacg actaatgttg
    30121 tatcagaacg ttggtcgtaa taacccaggc taatatgttc acgagcatta acttcaccac
    30181 cgttattaac cgacaggaac tgattaccgc catatccgcc gtaaacgccc ataaagaagg
    30241 tccctgcatt tacctgacta ccttcgccgc ttacattccc ctgcgtataa gtcagacgac
    30301 tggtgccctt aacatgaatt ttgccgccat tggagacatt aaaatagcct tgatcatgtc
    30361 tcatgcctga gtttgactca tacaaatcat cagcatataa ctcgctgcca gcattggaga
    30421 cattcaggaa ccccgtacct ttcagcaaaa agttaagcgg gccattgatt gttacctttc
    30481 ctccggtaat atccaaccca ccaccagaag cgagagtcag acgcggacgg gaagtatctg
    30541 tcgtattgat taccagattg gcattgtcag tcaccgtaat acggtaatcg atgctctggt
    30601 tctctttgcc aaaatcaata gctttatcct ggtttacgat gacatcatcg gcgaatacca
    30661 gagatgaata taaacttatc gcgctaatta aaacagcccg ggactttttt ccttttactc
    30721 tgctccccag ctccgagcag acgctccaac agttacggga cctgttccac tttagccgat
    30781 atattttatt catcacaatc tccagcaata catccaaata aaaccaagat tgcataaaca
    30841 gcaacgcatt aaatgccatt ctggttactg ttcatcgcat ataaaaattc aaaaattttg
    30901 catagcattg gcaaccacat atctgagtat gtagttcaga gggtaataaa atagcaagtt
    30961 attact
//
LOCUS       DAZFXT010000051        30400 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.051, whole genome shotgun sequence.
ACCESSION   DAZFXT010000051 DAZFXT010000000
VERSION     DAZFXT010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.051"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(66..797)
                     /gene="rlmB"
                     /locus_tag="ACHKW0_002455"
     CDS             complement(66..797)
                     /gene="rlmB"
                     /locus_tag="ACHKW0_002455"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HFO7475275.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(977..3418)
                     /gene="rnr"
                     /locus_tag="ACHKW0_002456"
     CDS             complement(977..3418)
                     /gene="rnr"
                     /locus_tag="ACHKW0_002456"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HFO7475276.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLSKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3457..3882)
                     /gene="nsrR"
                     /locus_tag="ACHKW0_002457"
     CDS             complement(3457..3882)
                     /gene="nsrR"
                     /locus_tag="ACHKW0_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HFO7475277.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4087..5385)
                     /gene="purA"
                     /locus_tag="ACHKW0_002458"
     CDS             complement(4087..5385)
                     /gene="purA"
                     /locus_tag="ACHKW0_002458"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HFO7475278.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5489..5686)
                     /gene="yjeT"
                     /locus_tag="ACHKW0_002459"
     CDS             complement(5489..5686)
                     /gene="yjeT"
                     /locus_tag="ACHKW0_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HFO7475279.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5768..6772)
                     /gene="hflC"
                     /locus_tag="ACHKW0_002460"
     CDS             complement(5768..6772)
                     /gene="hflC"
                     /locus_tag="ACHKW0_002460"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HFO7475280.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6775..8034)
                     /gene="hflK"
                     /locus_tag="ACHKW0_002461"
     CDS             complement(6775..8034)
                     /gene="hflK"
                     /locus_tag="ACHKW0_002461"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HFO7475281.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8120..9400)
                     /gene="hflX"
                     /locus_tag="ACHKW0_002462"
     CDS             complement(8120..9400)
                     /gene="hflX"
                     /locus_tag="ACHKW0_002462"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome rescue GTPase HflX"
                     /protein_id="HFO7475282.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9477..9785)
                     /gene="hfq"
                     /locus_tag="ACHKW0_002463"
     CDS             complement(9477..9785)
                     /gene="hfq"
                     /locus_tag="ACHKW0_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HFO7475283.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9871..10821)
                     /gene="miaA"
                     /locus_tag="ACHKW0_002464"
     CDS             complement(9871..10821)
                     /gene="miaA"
                     /locus_tag="ACHKW0_002464"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HFO7475284.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10814..12661)
                     /gene="mutL"
                     /locus_tag="ACHKW0_002465"
     CDS             complement(10814..12661)
                     /gene="mutL"
                     /locus_tag="ACHKW0_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HFO7475285.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12671..14008)
                     /gene="amiB"
                     /locus_tag="ACHKW0_002466"
     CDS             complement(12671..14008)
                     /gene="amiB"
                     /locus_tag="ACHKW0_002466"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HFO7475286.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14027..14488)
                     /gene="tsaE"
                     /locus_tag="ACHKW0_002467"
     CDS             complement(14027..14488)
                     /gene="tsaE"
                     /locus_tag="ACHKW0_002467"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HFO7475287.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14460..16007)
                     /gene="nnr"
                     /locus_tag="ACHKW0_002468"
     CDS             complement(14460..16007)
                     /gene="nnr"
                     /locus_tag="ACHKW0_002468"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HFO7475288.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16006..17145
                     /gene="queG"
                     /locus_tag="ACHKW0_002469"
     CDS             16006..17145
                     /gene="queG"
                     /locus_tag="ACHKW0_002469"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HFO7475289.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17128..17181)
                     /locus_tag="ACHKW0_002470"
     CDS             complement(17128..17181)
                     /locus_tag="ACHKW0_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475290.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17415..17490)
                     /locus_tag="ACHKW0_002471"
     tRNA            complement(17415..17490)
                     /locus_tag="ACHKW0_002471"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17455..17457),aa:Gly,seq:gcc)
     gene            complement(17526..17601)
                     /locus_tag="ACHKW0_002472"
     tRNA            complement(17526..17601)
                     /locus_tag="ACHKW0_002472"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17566..17568),aa:Gly,seq:gcc)
     gene            complement(17812..18357)
                     /gene="orn"
                     /locus_tag="ACHKW0_002473"
     CDS             complement(17812..18357)
                     /gene="orn"
                     /locus_tag="ACHKW0_002473"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HFO7475291.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18452..19504
                     /gene="rsgA"
                     /locus_tag="ACHKW0_002474"
     CDS             18452..19504
                     /gene="rsgA"
                     /locus_tag="ACHKW0_002474"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HFO7475292.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19601..20569
                     /gene="asd"
                     /locus_tag="ACHKW0_002475"
     CDS             19601..20569
                     /gene="asd"
                     /locus_tag="ACHKW0_002475"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HFO7475293.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEEN
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20591..23914
                     /gene="mscM"
                     /locus_tag="ACHKW0_002476"
     CDS             20591..23914
                     /gene="mscM"
                     /locus_tag="ACHKW0_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HFO7475294.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPIRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24065..25567)
                     /gene="yjeM"
                     /locus_tag="ACHKW0_002477"
     CDS             complement(24065..25567)
                     /gene="yjeM"
                     /locus_tag="ACHKW0_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HFO7475295.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
     gene            complement(25786..26763)
                     /gene="epmA"
                     /locus_tag="ACHKW0_002478"
     CDS             complement(25786..26763)
                     /gene="epmA"
                     /locus_tag="ACHKW0_002478"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HFO7475296.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27088..28896
                     /gene="frdA"
                     /locus_tag="ACHKW0_002479"
     CDS             27088..28896
                     /gene="frdA"
                     /locus_tag="ACHKW0_002479"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HFO7475297.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGVVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            28889..29623
                     /gene="frdB"
                     /locus_tag="ACHKW0_002480"
     CDS             28889..29623
                     /gene="frdB"
                     /locus_tag="ACHKW0_002480"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HFO7475298.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            29634..30029
                     /gene="frdC"
                     /locus_tag="ACHKW0_002481"
     CDS             29634..30029
                     /gene="frdC"
                     /locus_tag="ACHKW0_002481"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HFO7475299.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30040..30399
                     /gene="frdD"
                     /locus_tag="ACHKW0_002482"
     CDS             30040..30399
                     /gene="frdD"
                     /locus_tag="ACHKW0_002482"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HFO7475300.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
BASE COUNT         7121 a         8430 c         7782 g         7067 t
ORIGIN      
        1 atcttaccag ctatagacaa aaaaaaacca tccaaatctg gatggctttt cataattcag
       61 acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc aaccgaaacg
      121 ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca atgttcacgg
      181 gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaggcg tccggtcatt
      241 ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca gatattctct
      301 tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg aacgctttct
      361 gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg atctttcggc
      421 acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc gaggttgtgc
      481 ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc gatcagatcc
      541 ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat gccctgatgc
      601 acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat aaccacgccc
      661 tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc ttttaaaata
      721 aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc gtggatgccg
      781 taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc actacgttta
      841 tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg ccggataagg
      901 cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa ccctcttctt
      961 ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc gctttggtcg
     1021 ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg tctttcttcg
     1081 ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca aagtttacct
     1141 ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt ttcgctttct
     1201 cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga ctaaagtcga
     1261 ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg cccaggcgat
     1321 aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag cgatagtagt
     1381 cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc agacggacga
     1441 agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct acctggtcga
     1501 gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg gcacgacgtt
     1561 ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa tggtagccgc
     1621 cggtttcagt ggtgttgccc tgatgcccct gctctttcga cagcagatat ttaatggcgc
     1681 ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca taggactgca
     1741 atgccagacc gaagtgacca cggttttctg gatcgtaaat cgcctgtttc atcgagcgta
     1801 gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc agcagttccg
     1861 cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc tccgccagca
     1921 ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg aacagtgccg
     1981 gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc atgcactctt
     2041 caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata cggcgttcag
     2101 cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt tcttcacggg
     2161 ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc agcggggcgt
     2221 actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg taggtcagac
     2281 gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg cctttcgacg
     2341 aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc gaacacaggc
     2401 cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac accgacgtgc
     2461 cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag ctgacgtcgg
     2521 caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa actgcatcgt
     2581 caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc aaatcgacac
     2641 ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc ggcaacctgt tgctcaacag
     2701 cctgcggcca gatgtacgga atttcatggg tacgcagagc gatatcaacc gccatgccgg
     2761 tgcccatatt atcgccaagg acttcgacga ttttacccac cgctttggtg cggcgagtcg
     2821 gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg atctgatcgg
     2881 gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag ccgacgcccg
     2941 cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg cggacaatac
     3001 gcgcttcacg acgaccttta cggtcagcgc ccagcggctg agccagcacc tgatcgccat
     3061 gaatgcaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc ccttcaaccc
     3121 gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg tcgaggcgtt
     3181 ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc tccatcgcgc
     3241 gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt tctaccgcca
     3301 gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata aattcccgac
     3361 tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct tgtgacatct
     3421 cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca ataatttata
     3481 aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt ccgtaagaaa
     3541 actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa tgtggcaaaa
     3601 ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat caccaatacg
     3661 tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa cagcagtcac
     3721 gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg agacgccgta
     3781 gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg ccatgtagat
     3841 cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct caaaagggaa
     3901 atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt cgctaattta
     3961 tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag cgtaatggct
     4021 taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct gccaggcgta
     4081 ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat ccggaccggt
     4141 agagatgata tcgatcggca caccagtcag ctcttcaata cgcttgatgt agttcagcgc
     4201 cgcctgcggc agaccgctac gatctttcac gccgaaggtg gattcagacc agcccggcat
     4261 ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag tggtcgtcac
     4321 ttcacgacca tccggcatac ggtaagccac acagagtttc acctctttca ggccatccag
     4381 aacgtccagt ttagtcaggc agaagccaga cagggagttc agctgtaccg cacgacgaac
     4441 ggcaacggtg tccagccagc cggtacgacg acgacgcccc gtagttgcgc cgaattcgtt
     4501 accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga acggacctgc
     4561 acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat aacgcgggcc
     4621 caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta cgtacggata
     4681 agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga cgaaatcgcc
     4741 acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag tcaggatgtc
     4801 ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag ctttgtagta
     4861 gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag cgaaggtttc
     4921 tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt cataagcagg
     4981 cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct cacgcgcgtt
     5041 atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca gcagacgctc
     5101 acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg cggccggaga
     5161 cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac ctgatggaat
     5221 aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt tgtgaccgcc
     5281 ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct tacctttacc
     5341 ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc aaaatcaccg
     5401 tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt ttgcactcaa
     5461 aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac atgtagtaga
     5521 ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta tcgggcaaat
     5581 tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc cctaaacctt
     5641 ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat tatccttata
     5701 gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct gtaccgcagt
     5761 cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa tcgctatccg
     5821 ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca cgcaggctac
     5881 ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt ttggctgctt
     5941 cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc agcgttctgg
     6001 tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct tgtgaacggt
     6061 gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct tcagacactt
     6121 cggtcggcag gttgatctgc ttaattcgca catcgacaac ctcaataccc agcgccgcca
     6181 tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg cgctctgccg
     6241 cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta cccgcagaac
     6301 cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg gtgacgatat
     6361 ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta cgtttcaaca
     6421 gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg ctgaaatcgc
     6481 tgatgcgcca tttgatgtaa gagtcgacga tcaggtcttt cttctctttg gtcacaaagc
     6541 ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc gtttcaatga
     6601 acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca tcgtcacgca
     6661 gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca aagacagaca
     6721 tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc atcgttattc
     6781 cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca taatatcgcc
     6841 ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg ctggcggcag
     6901 acgcagcaga ttgctggtac cgttatcgct cttcgccgca ggggcgttac cgcctttcag
     6961 catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca gcactttgcg
     7021 ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag taatttccgg
     7081 cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct gagcttccag
     7141 gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct gaccgttcgc
     7201 acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct gttcgttttc
     7261 acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac gagcagcctg
     7321 gaagttgacg tccagcagcg tgatacccat gtcatacgga cggatagtct cttccagttc
     7381 gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt ccatggtgta
     7441 tttaccgata accccgcgca gggcgctgtc ggtagcctga cgcaggctgt catccgggct
     7501 ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt tcatctcaac
     7561 gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt cacgcacggc
     7621 ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca gacccggctc
     7681 aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt ctttaatggt
     7741 atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg taacgacacg
     7801 accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc cggtgccttt
     7861 accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat ctaaatcagg
     7921 tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc caggtttgct
     7981 gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg ccatgtttgt
     8041 gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga tatgactcca
     8101 cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct tgtttacaga
     8161 ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg tcctcctcca
     8221 tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgc ccttcctgcg
     8281 gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc aaagcctgaa
     8341 aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc ggtttgttct
     8401 cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg ttcatcacca
     8461 gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc gcttcgatgt
     8521 tttcttgtac acgcacatcc gccgcatcaa tgacgtgcag cagtaatgtg gcttgccgcg
     8581 tctcttgtaa cgtggcttta aatgccgcca ccagatcgtg cggcaggtgg cgaataaacc
     8641 ctacggtatc tgcaagtacg gtttcaccga catctgcaac gtcaatacgc cgcaacgtcg
     8701 ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg atgcgattga
     8761 aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga acgtcggctt
     8821 tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttccagg cgtgactgta
     8881 tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt tcacccggac
     8941 cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc accaggcgcg
     9001 tagccagatg gcgcagctgc gccagctcaa cctgcaactt accctcatgg gtacgcgcac
     9061 gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac tcgcacaaac
     9121 gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg accgaagcac
     9181 ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca aaatactttg
     9241 ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg gcggaagaga
     9301 ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa tagatgtgta
     9361 ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc ctctaaatag
     9421 atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc gaaaccttat
     9481 tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgctgctacc atgatggtag
     9541 ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg agacgggaca
     9601 acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa caggatcacg
     9661 aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa ataaatagaa
     9721 actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga ttgcccctta
     9781 gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat tctgaaaaaa
     9841 ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa caacctgtaa
     9901 tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc cttcccaacc
     9961 acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac ctcgataaac
    10021 catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat aacccacgca
    10081 acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc ggacttctgc
    10141 ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat ggagcagttc
    10201 acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt ctcctgacgt
    10261 ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg cccgggaaag
    10321 cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct cctgaagttg
    10381 acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg cccgcacttc
    10441 cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat acaacatcgt
    10501 accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt ccgccagcgc
    10561 atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat ccagcaatcg
    10621 gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat ccatcccttt
    10681 gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac gcagctcaat
    10741 ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct taggcaggct
    10801 cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa tcaacagatt
    10861 gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc acgtccgcca
    10921 gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga tttcgtgcaa
    10981 tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag cctatcagtt
    11041 caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc ctgatggtca
    11101 catgctgtgc atctgactgg aaatcaatac ccaattccgc tagggcagac tgcgcttttt
    11161 ctaatgccga tttttcttcg gcagaaactt ttagccgcaa cggaatcagc agcggctggg
    11221 cgcaaacggg cgcttcaccc ggcgtcaatt gtgcctgacg tagccaacgt tctgccactg
    11281 gcaaggataa aagtgaaatg ttgccatcgc gctccagcaa cgcacagtcg gaatggacga
    11341 tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc tgcggttccg
    11401 gcgcttttgg tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac acttcacctt
    11461 gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga ctgcctgatg
    11521 ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct gcaaagtgat
    11581 tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg ggttcatcgt
    11641 ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc tgatagataa
    11701 aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg tgcacgttga
    11761 cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc tgatcggccc
    11821 ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg cgcatcatgc
    11881 gaccgttcac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga tttggatcgg
    11941 ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc gcttgttcaa
    12001 gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct tccggcactg
    12061 cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg tcgaaacgcg
    12121 ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc tcggtgcgca
    12181 ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc gtcgtcccca
    12241 caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca taggcctgcc
    12301 aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc gaactgatac
    12361 tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg tccagagagg
    12421 cgattttact ggtggcatga cgcgccagcg ccagcgccag ctcgtctttt ttgataccgc
    12481 agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca atatcgatac
    12541 gtgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac gcaggtcgct
    12601 cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc tgaattggca
    12661 tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg gccgtttgtg
    12721 ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg cgcaggcctt
    12781 tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa cgttcttcgc
    12841 tgttgttgct gataaaaccg gtttcgacca gtactgatgg gatatccggc gaacgcagaa
    12901 cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt tgcaactgac
    12961 tgatcatact ggtcgctaca tcatacccta cccgttggga atgaccgaac tgtaaatcca
    13021 gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatca cccgccccac
    13081 ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg ctgttggcgc
    13141 gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt gcggcatcag
    13201 cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcactg cgccccatca
    13261 ccgagataaa gtaatccccg tcacgggtta aaacgccctt aaacatcgga tcgtcattga
    13321 gcaaagtacg caatttacgc gcgatggcga tggtgacatt tttctcccgc gtaccaccgg
    13381 ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg ataattttat
    13441 cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg ctgataacac
    13501 ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg acgcgcggtg
    13561 cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggcggagga ggaggtggcg
    13621 gcgcatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc cgcttcaccg
    13681 cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt gcatctttag
    13741 gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac ggcagtccct
    13801 gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga tggctaaacg
    13861 cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta ccgttagaaa
    13921 cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc agcgtcgcta
    13981 ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa ccggctaaac
    14041 gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca cggccttgtg
    14101 cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacaccagta ccttgttgtg
    14161 gccactccac caggcagatg gcatcgttgg caaaataatc gcgaatcccc ataaactcca
    14221 gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa ttgtcgagcg
    14281 tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga cccagagctt
    14341 gtaaaaagcc ccggctaaag gtggttttac ctgcgcctaa atcgccatac agatagatta
    14401 cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt gcctgctcat
    14461 cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg gttaacaata
    14521 cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa acgcgccgcc
    14581 agtacgtcag ctgcagcacc gtgcgcgaca cagcccgcac aggcggcatc atacggcgac
    14641 agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat gccgccgctc
    14701 gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc ggcgaccacg
    14761 gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg tttggcgcaa
    14821 tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc ggcctcgcca
    14881 ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag caggttcaat
    14941 gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag tgcttttttc
    15001 ccccactctt gctggcccag accgggacca atcaccacca catcggccca ttccaggctt
    15061 tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc agtcagcagc
    15121 ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc acgtagcgcc
    15181 gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat aattaccagc
    15241 cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt ttagccagtg agaaagttgt
    15301 tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccaactatc cagccccagt
    15361 gagtcaaaat gcagttgtcc ggtaacatcc cgcgcttttc cagtgagcaa gcctggtttc
    15421 agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt agcgccggtt
    15481 tcagccagca ggccggaagg gatatcaacc gccgcaatcg gcgcaggatg ggaattagcg
    15541 tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt accgagcagc
    15601 gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg gatctcgcct
    15661 cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg tttgtcgctc
    15721 tcctgggcca gcagcgtgac ctcaatgccg accgctgtgg ccagccgcgc gaccacgtag
    15781 ccatcgccgc cgttattacc atgaccgcac agcaccagcc aatggcgggc gtcaggatac
    15841 gccgaacgac acacctggaa tgcggcttcg ccagcgcgaa gcatcagctc atagagtgta
    15901 agccccagcg catctgccgc ctcacgttct ccgcggcgga tatcgtcggc gtgccagacg
    15961 gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc agagcccctc
    16021 gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg ctttcagcag
    16081 gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc atggctggac
    16141 aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg cgctcgcccc
    16201 catgagttat tgcccggtac gctgcgcgtg atcagcgtgc ggatgaatta ccttcctgct
    16261 aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag ccgttatgcg
    16321 ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg cgagatgatt
    16381 cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc tattctcgag
    16441 cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact tatcctcaat
    16501 cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc gctgcccgtg
    16561 gatcaaccag tcgaggaagg atgcggtaaa tgcgtggcct gtatgacgat ttgcccgacc
    16621 ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta tctcaccatc
    16681 gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg tatttacggt
    16741 tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cacaactcac cacagaagag
    16801 gatttcagcc cgcgtaagcc gctacacgca ccggaactca ttgagttatt cgcctggagc
    16861 gaagagaagt ttttaaaagt cacggaagga tcggcgattc gtcgtattgg tcacctgcgt
    16921 tggctgcgta atatcgccgt agcattaggc aatgcccctt gggatgaaac gattttgaca
    16981 gcgctggaaa gtcgtaaagg tgagcaccca cttctcgatg agcacatagc gtgggcgatt
    17041 gcgcagcaaa tagagagacg aaatgcgtgc atagtcgaag tgcaattgcc gaaaaaacag
    17101 cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat ccacagcttg
    17161 tgaataaaaa taaaaacgca ttgcaattca agacgaataa aatcgtcaag tgatcgactc
    17221 gacaaaacgg aatcttaatt tttaacttat ctatcaatag gttaaaaaaa tactattcac
    17281 cgtgcgaagt ttctttgaaa ttaagattac agcctgaggc tgtggataac tctgtttaca
    17341 agagtttgtc agagacaacg aaaaccagca ataacgcatc gcatcgctgt ggatatttta
    17401 ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga ccttggcaag
    17461 gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac agcactttca
    17521 aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc
    17581 taccaactga gctattcccg cgtactactt aattttgctt tcgtaatttt cgcatttctg
    17641 cgtcgttacg ggaggcgcat tatacgagaa atctttccct ctgcaacccc ccaaaaaagt
    17701 tttttttgcg tttttatctc aagtgctgaa attatcagca agacgttcaa tttagcgtca
    17761 aaatcacgcc gccgcaagtg gattccggcg cttacgtgac caggaaaaat tttacagctt
    17821 gataaaatgc tcgcggtagt aagccagctc cgccaccgat tcacggatat catccatcgc
    17881 ctgatgcgtc ccctgcttgg taaaaccatc cagaatttcc ggcttccagc ggcgcgccag
    17941 ctctttcagg gtgctgacat cgagataacg gtagtggaag taggcttcca gctccggcat
    18001 gtatttaaac aggaaacgac ggtcctgacc gatgctgtta ccgcaaatcg gcgattttcc
    18061 cgcaggcacc cactgtttta aaaattcgag cgttgccagt tcagcttccc gatcgcccat
    18121 cgtgctcgct ttcacgcgct ctaccagccc gctggcggta tgggtgcgca cgttccagtc
    18181 atccatcagc gccagctgtt catcagactg gtgtactgca atggtcggcc cttctgccag
    18241 aatattcagg ttggcatcgg tcaccagcgt ggcaatctca ataatgcgat cgcgctcggg
    18301 atccagaccg gtcatctcaa gatcgatcca aatcaggttg ttttcattgg cactcatgct
    18361 attttccacc cattcacagt gactatattg atccacaatt gcgtgtatca tagatgtttt
    18421 gcccatcagg ggcgaccagg agtctgtacg attgagtaaa aataaactct ccaaaggcca
    18481 gcagcgccgc gtgaacgcca atcaccagcg tcgtcttaaa acgtctaagg agaagcccga
    18541 ctacgacgac aatctgtttg gcgagcctga tgaaggtatc gtcatcagcc gctttggtat
    18601 gcacgctgat gtggaatccg ccgatggcga cgttcaccgc tgcaatattc gccgtaccat
    18661 ccgttcgctg gtaaccggcg accgcgtagt ctggcgtccg ggtaaaccgg cggcggaagg
    18721 cgtaaatgtc aaagggatcg tggaagcggt gcatgagcgt acctcggtgt tgacgcgtcc
    18781 ggatttctac gacggcgtaa aacctattgc cgccaacatc gaccagattg tcattgtctc
    18841 cgccattttg ccggagctgt cgctcaatat tatcgaccgt tacctggtgg cctgcgaaac
    18901 cttgcagatt gagccgatta ttgtgctcaa caagatagac ctgctggacg acgaaggcat
    18961 ggcgttcgtc aacgagcaga tggatatcta ccgcaatatc ggttatcgcg tattgatggt
    19021 ttccagccat actcaggatg ggctaaaacc gctggaagag gcgttgaccg ggcgcatcag
    19081 catttttgcc gggcagtctg gcgtcggcaa atccagcctg ctgaatgcgt tgctggggct
    19141 gcaaaaagag atcctgacca acgatgtctc tgacaactcg gggctcggtc agcacaccac
    19201 gaccgccgct cggctgtatc acttcccgca cggcggtgat gtgattgatt cccctggtgt
    19261 gcgtgagttc ggcctctggc acctggagcc ggaacaaatc actcagggct ttgtcgaatt
    19321 ccatgactat ttaggtctgt gtaaatatcg cgattgcaaa cacgataccg atccgggctg
    19381 cgctatccgg gaagcggttg aggaagggaa aatcgcggaa acccgtttcg aaaactatca
    19441 ccgtattctg gaaagcatgg cgcaggtaaa aacgcgtaaa aacttttctg atacggatga
    19501 ctgacaatta agctaaccgt cgttagaatc gtcccctttt tttcaggatc cggcatgttg
    19561 gccggatcag gaacgacaaa acaatggcct ggaggctacc ttgttaaatt catttaaact
    19621 ttcgctacag tacattctgc cgaaactatg gcttactcgc ctggcgggtt ggggcgcaag
    19681 caagcgggca ggatggctga caaaactggt tatcgatctg ttcgttaaat actacaaggt
    19741 cgacatgaaa gaggcgcaaa agccggacac cgccagctac cgcaccttta acgaattctt
    19801 tgtccgtccg ctgcgtgacg aagtacgccc aatcgatacc gatccgaatg tcctggtcat
    19861 gcctgccgat ggcgttatca gccagttggg taaaatcgaa gaaaataaaa tcctgcaagc
    19921 caaaggccac aactacagcc tcgaagccct gctggcaggc aactatctga tggcggacct
    19981 gttccgcaac ggtacgtttg tgaccactta cctctccccg cgtgactacc atcgcgtaca
    20041 catgccgtgc aacggtattc tgcgtgagat gatctacgtg ccgggcgatc tcttctccgt
    20101 taaccatctc accgctcaga acgtgccgaa tctgtttgcc cgtaacgaac gcgtgatttg
    20161 cctgttcgat accgaatttg gcccgatggc gcagattctg gtcggggcga cgattgtagg
    20221 cagcattgag acggtctggg cgggcaccat tacgccgccg cgcgaaggta tcatcaagcg
    20281 ctggacctgg cctgccgggg aaaacgacgg ttctgtggca ctgctgaaag gccaggaaat
    20341 gggccgcttt aaactcggct ctaccgttat caacctgttt gcaccgggta aagtgaatct
    20401 ggttgagcaa ctggaaagcc tgtctgtcac gaaaattggc cagccgctgg cagtatctac
    20461 cgaaaccttt gttacgccag acgctgaacc cgccccactt cctgctgaag agatcgaagc
    20521 agaacacgac gccagcccat tggttgacga taaaaaagac caggtctaat ccatcaaagg
    20581 aaacgctgac gtgcgcctga ttatcacttt tctgatggcc tggtgcctca gttggggggc
    20641 gtacgccgcg acggcccccg atagcaaaca aatcactcag gaactggagc aggcaaaagc
    20701 ggcgaaaccc gcacagccgg aagtcgtaga ggcgctccag tctgccttaa atgcgcttga
    20761 ggaacgaaaa ggttcccttg agcgcatcaa acaatatcag caagttatcg ataattatcc
    20821 gaaactctcc gctactctgc gcgcacaatt aaacaacatg cgtgacgagc cgcgcagcgt
    20881 gtcgccggga atgtctaccg acgcgctgaa tcaggaaatt ctccaggtca gcagccagtt
    20941 gctggataaa agccgtcagg cccagcaaga gcaggagcgc gcccgcgaga ttgccgattc
    21001 gctgaatcaa ctgccgcaac agcaaaccga cgcccgccgt cagttaaatg agatcgagcg
    21061 ccgcctggga acgcttactg gcaatacccc gctcaatcag gcacaaaatt tcgcgttgca
    21121 gtctgactcc gcacgcctga aagcgctcgt tgatgaactg gaactggcgc agctgtctgc
    21181 caataaccgt caggaattag cgcgcttacg ctcagagctg gcggaaaaag agagccagca
    21241 actggatgcg tatttgcagg ccttacgtaa tcagttgaac agccaacgtc agcttgaggc
    21301 ggagcgggcg ctggaaagta ccgaattgct ggcagaaaac agcgccgatt tgccgaaaga
    21361 catcgtcgcg caattcaaaa ttaaccgcga actatcggcg gcactgaatc aacaggcgca
    21421 gcggatggat ctcgttgcct cgcaacagcg ccaggccacc agccagacgt tacaggttcg
    21481 gcaggcgctg aatacgctgc gtgaacagtc gcaatggctg ggatcgtcca acctgctcgg
    21541 cgaagcgttg cgggcgcagg tggcacggct gccggaaatg ccgaaaccac aacagcttga
    21601 taccgaaatg gcgcagttgc gtgtgcaacg gttacgttat gaggatctgc ttaataaaca
    21661 gccgctgcta cggcaaattc atcaggccga cggtcagccg ctgaccgccg agcaaaaccg
    21721 tattctggaa gcacaactgc gcactcagcg tgagttgctg aactcattgt tgcagggtgg
    21781 cgacacgcta ctactggaac tgaccaagct gaaagtctcc aacgggcaac tggaggatgc
    21841 gctgaaagag gtgaacgaag caacgcaccg ctatctgttc tggacctctg acgtgcgccc
    21901 gatgaccatc gcctggccgc tggaaatcgc ccaggatctg cgtcgtctca tttcgctgga
    21961 caccttcagt cagttgggca aagccagtgt gatgatgctg accagcaaag agacgatttt
    22021 gccgctgttt ggcgcgttga ttctggtcgg ttgcagtatt tactcgcgcc gctatttcac
    22081 ccgttttctt gaacgttcgg cggcgaaagt cggcaaagtg actcaggatc acttctggct
    22141 gacgttgcgc actcttttct ggtcgattct cgtcgcgtca ccgctgccgg tgctgtggat
    22201 gacgctgggt tacggcttgc gcgaggcgtg gccttatccg ctggcggtcg cgattggcga
    22261 tggcgtaacg gccaccgtgc cgctgctgtg ggtagtgatg atttgcgcca cctttgcccg
    22321 cccgaacggc ttgtttatcg ctcattttgg ctggccgcgc gaacgtgttt cccgtgggat
    22381 gcgctactac ctgatgagca tcgggcttat tgtgccgctg attatggcgc tgatgatgtt
    22441 cgataacctc gacgaccgtg aattctccgg ttcgctggga cggctttgct ttatcctcat
    22501 ttgcggtgcg ctggcggtgg tcaccctcag cctgaaaaag gccgggatcc cgctgtacct
    22561 caacaaagag ggcagcggcg acaacattac caaccatatg ctgtggaaca tgatgattgg
    22621 cgcaccactg gttgccattc tggcgtcagc ggtgggttat ctggcaacgg cgcaggcact
    22681 gttagcgagg cttgaaacct cggttgccat ctggttcctg ctactggtgg tttatcacgt
    22741 tatccgccgc tggatgctga tccagcgtcg caggctggcg tttgaccggg cgaagcatcg
    22801 ccgggcagag atgttagcgc aacgcgcgcg tggcgaagag gaagcgcatc atcacagtag
    22861 cccggaaggg gcaattgaag tcgatgaaag cgaagtcgat ctcgatgcca tcagtgcgca
    22921 atccttgcgg ctggtgcgct caattttgat gttgatcgcc ctgctttctg tcattgtgct
    22981 gtggtcagaa atccattccg ctttcggctt cctcgaaaat atttcgctgt gggatgtcac
    23041 ctccacggta cagggcgtag aaagtctgga gccaattacc ctcggtgcgg tgctgattgc
    23101 cattctggtg tttatcatca ccacgcagct ggtgcgcaac ctgcccgcgc tgctggaact
    23161 ggcgattttg cagcacctgg atttaacgcc gggtacgggc tacgccatca ccaccatcac
    23221 caaatatctg ctgatgctga ttggcgggct ggtcggcttc tcgatgattg gtattgagtg
    23281 gtcgaaattg cagtggctgg ttgccgcgct cggtgttggt ctcggttttg gtttgcagga
    23341 gattttcgcc aactttatct ctggcctgat tatcctgttc gaaaaaccga ttcgcattgg
    23401 cgatacggta acgatccgcg atcttaccgg tagcgtgacg aaaattaaca cccgcgccac
    23461 caccatcagc gactgggacc gtaaagagat aatcgtgccg aacaaggcgt ttattaccga
    23521 gcagtttatc aactggtcgc tctctgactc ggtcacgcgc gtggtgttga cgataccggc
    23581 ccctgccgat gccaatagcg aagaagtgac ggaaatcctg ctcaccgcag cgcgtcgctg
    23641 ctcgctggtg atcgacaacc cggcaccgga agtcttcctg gtggatctgc aacaggggat
    23701 tcagattttc gagctgcgta tttacgccgc tgagatgggt caccgtatgc cgatacgcca
    23761 tgagatccac cagctgattc tggcaggctt ccacgcccac ggtatcgata tgccattccc
    23821 gcccttccag atgcgtctgg aaagcctcaa cggtaagcaa acggggagaa cattaacttc
    23881 tgcgggcaaa ggtcgtcagg cgggaagttt gtaatgcatg aagctgccaa atgatgtcaa
    23941 cgtggctcat ttagcagctt aacatcatct tcagttatca ccggccctgg agagtatcat
    24001 ccatacgcat tacttcctga caactcatcg ccggagagac gatgagttgc aggatttcat
    24061 atgatcagtc gagagctaac tcaggtttat tcgccatgcg actgcaatag ttctggtaaa
    24121 tcgccatcgc taacagcgag aagaagacag ggccgccaat catccacaat gtgctgtccc
    24181 agtctccggc ttcaaccaca ggttgaatga tggtgaagac gttcgcaaat gtcaccacca
    24241 gtacgacaac caccgtcgca atcattgccg acatacgcgt tttgaaaatc acaaatggtc
    24301 tgtcgagatc ctgacgtgct ttaaagaatg ggaaagccag cgcgaggaac aggtaaggaa
    24361 gcgtcataga cacgttcgcc atcagcgtca gcttgttaaa gaacgccgat gcggtaccgc
    24421 caccaaacga aaccagcagg atgaagacag taaccaaccc gcactgcatc cacatggcga
    24481 tagaaggcat ccccatcgca ttcaggcgcg tcatcggttc cggccacaac gctttcggcg
    24541 tcccctggat gatggctttc agcggtgaat agcaaagcgt aaagaacgcc ccggtataag
    24601 cgaggaacat cgaaagtccg gtaatacgcg caaaccatac acccagcgac aatgacgctt
    24661 caggtgacaa atgcagtgcg ttacccagcg tcaccccgag gctcttcatc agcacatagg
    24721 taatattgcc gaggttaacg gaaccattac ttaatacctg ctgccagttt gtgctgacac
    24781 cccataaaaa tattgccagc gaataaccga ttgaaataac aatagcggca aaaacaatac
    24841 ctttggcaaa gttcttttct ggattttccg ttttatcgac cagaccacca accgcttcaa
    24901 ttccgccata ggcaaaaata gcaaatacca caaacgatag cattgccaga ccggactgat
    24961 aacctgggtt cggtgatgca aggaaattaa tatcctgcgc gaaatgccca ccatttaata
    25021 acaaaatagt aatgcttact aacagcaata ctaaattcag acacattact gcaataccgc
    25081 ccaccgcagt aatgcgggca attttattaa tcccctttga agcaacgacg gtgaccagaa
    25141 tcatccatgc tactgccagc agaccaacca cctgcgtagg ctccagtccg gcaatacgcc
    25201 agtgctgggt catgtcgcta ccatagagga atgttgagaa cggtacccaa actttcgcgg
    25261 aggtactcac catccagatg atataagagg aaaaccacat aaacgtacca atgaaggcaa
    25321 aacgtggtcc gacactatta ttcatccagg aatagatacc accttcttct ttgcgataag
    25381 cagctcccat ttcagccatc attaaggcga atggaataaa gaataacaat gcagaaaata
    25441 tataaaaggg aatcgcacta taacccatta agtaataagc cgatgggcta ttggcaaatc
    25501 caaatacgga agtaaagatc atcaatatga gtcctatcag actcatcttt tttatcgtgt
    25561 gaggcatgaa accatccttc atttcagcgc gtgtataaca cgtgcccact gaactgcact
    25621 gcgcaacatt gagcaggcag cgtaaaaacc aatacagaat tgatacctgg atgatatcaa
    25681 atatccagtc ttaaattgtg gcctttaacg gaaaaatgaa aacatatgct acagaatggc
    25741 gcttatcacg ccattcttcg ctgttaattc agtaattttt cagaattatg cccggtcaac
    25801 gctaaaggcg atgacttcag ccagtgtctc cgcgcccagc gccaacatca ccagacgatc
    25861 aacacctaat gccacgccgg aacagtcagg cataccgact ttcaaggctt caatcagatt
    25921 ctggtcaatg gggtgctgcg gcaaaccgcg cgccgcgcgc ttacggttat cttgttcaaa
    25981 gcgttgttgc tgctcacggg catccgtcaa ttcatggaaa ccattcgcca gctcaatacc
    26041 tttataataa acctcaaagc gttcagcgac ccgatgatct tcggtactga tttgcgccag
    26101 tgatgcctgg ctggctggaa agtggtacac aaaggtcggt ttttctttgc caatatttgg
    26161 ctctacgcca aaggtaaaca gcaattgtag cagcgtgtcg cggtcttctt cggtatcagc
    26221 aacattgctc aaatccagtt tcgctgcgac ttcccgcagt tgcgttttgt cggcagagag
    26281 cgggtcaatt tccagataac gcaagaaagc ttgttgataa gaaaggcttt ctgctgccgg
    26341 gcagtccagc acctgttgta agagatcgtc cacctcgttc atcaaccggt acatatcata
    26401 gtgcggtcga taccactcca gcatagtgaa ctcagggttg tgataacgcc ccatctcttc
    26461 attacggaag ctgcggcaca gctggaatac cggcccacaa ccggcaacca gcaggcgttt
    26521 catatggtat tccgggctgg tcattaacca gagattcatc ccctgcgaat gcccggggcc
    26581 aacgaaacgt gtctcaaacg ggaccaaatg aatatcggtt accgtcgcct ggctcataca
    26641 aggcgtctcc acctccagca ctccacgatc ggcaaagaaa cgacggatct ccgccataat
    26701 cgccgcgcgt tttaataagt taggaatgga tgcgctcggc tgccaggatg ccgtttcgct
    26761 catagttaaa tctccagttt ttgacaaggg cacgaagtct actcgcaacg cgacggcgag
    26821 acaaatttta cgcaggaatc aaacagcggt gggcagtgac taaaaaaagc acgatctgat
    26881 ggtttagtaa ttaaattaat catcttcagt gataatttag ccctcttgcg cactaaaaaa
    26941 atcgatctcg tcaaatttca gacttatcca tcagactata ctgttgtacc tataaaggag
    27001 cagtggaata gcgttcgcag accgtaactt tcaggcactt accctgaagt acggggctgt
    27061 gggataaaaa caatctggag gaatgtcgtg caaacctttc aagccgatct tgccattgta
    27121 ggcgccggtg gcgcgggatt acgtgctgca attgctgccg cgcaggcaaa tccaaatgca
    27181 aaaatcgcac taatctcaaa agtatacccg atgcgtagcc ataccgttgc tgcagaaggg
    27241 ggctccgccg ctgtcgcgca ggatcatgac agcttcgaat atcactttca cgatacagta
    27301 gcgggtggcg actggttgtg tgagcaggat gtcgtggatt atttcgtcca ccactgccca
    27361 accgaaatga cccaactgga actgtggggg tgcccatgga gccgtcgccc ggatggtagc
    27421 gtcaacgtac gtcgcttcgg cggcatgaaa atcgagcgca cctggttcgc cgccgataag
    27481 accggcttcc atatgctgca cacgctgttc cagacctctc tgcaattccc gcagatccag
    27541 cgttttgacg aacatttcgt gctggatatt ctggttgatg atggtcatgt tcgcggcgtg
    27601 gtagcaatga acatgatgga aggcacgctg gtgcagatcc gtgctaacgc ggtcgttatg
    27661 gctactggcg gtgcgggtcg cgtttatcgt tacaacacca acggcggcat cgttaccggt
    27721 gacggtatgg gtatggcgct aagccacggc gttccgctgc gtgacatgga attcgttcag
    27781 tatcacccaa ccggtctgcc aggttccggt atcctgatga ccgaaggctg ccgcggtgaa
    27841 ggcggtattc tggtcaacaa aaatggctac cgttatctgc aagattacgg catgggcccg
    27901 gaaactccgc tgggcgagcc gaaaaacaaa tatatggaac tgggtccacg cgacaaagtt
    27961 tctcaggcct tctggcacga atggcgtaaa ggcaacacca tctccacgcc gcgtggcgat
    28021 gtggtttatc tcgacttgcg tcacctcggc gagaaaaaac tgcatgaacg tctgccgttc
    28081 atctgcgaac tggcgaaagc gtacgttggc gtcgatccgg ttaaagaacc gattccggta
    28141 cgtccgaccg cacactacac catgggcggt atcgaaaccg atcagaactg tgaaacccgc
    28201 attaaaggtc tgttcgccgt gggtgaatgt tcctctgttg gtctgcacgg tgcaaaccgt
    28261 ctgggttcta actccctggc ggaactggtg gtcttcggcc gtctggccgg tgaacaagcg
    28321 acagagcgtg cagcaactgc cggtaatggc aacgaagcgg caattgaagc gcaggcagct
    28381 ggcgttgaac aacgtctgaa agatctggtt aaccaggatg gcggcgaaaa ctgggcgaag
    28441 atccgcgacg aaatgggcct ggcaatggaa gaaggttgcg gtatctaccg tacgccggaa
    28501 ctgatgcaga aaaccatcga caagctggca gagctgcagg aacgcttcaa gcgcgtgcgc
    28561 atcaccgaca cctccagcgt gttcaacacc gacctgctct acaccattga actgggccac
    28621 ggtctgaacg ttgctgaatg tatggcgcac tccgcaatgg cacgtaaaga gtcccgcggc
    28681 gcacaccagc gtctggacga aggttgcacc gagcgtgacg acgtcaactt cctcaaacac
    28741 accctcgcct tccgcgatgc tgatggcacg actcgcctgg agtacagcga cgtgaagatt
    28801 actacgctgc cgccagctaa acgcgtttac ggtggcgaag cggatgcagc cgataaggcg
    28861 gaagcagcca ataagaagga gaaggcgaat ggctgagatg aaaaacctga aaattgaggt
    28921 ggtgcgctat aacccggaag tcgataccgc accgcatagc gcattctatg aagtgcctta
    28981 tgacgcaact acctcattac tggatgcgct gggctacatc aaagacaacc tggcaccgga
    29041 cctgagttac cgctggtcct gccgtatggc gatttgtggt tcctgcggca tgatggttaa
    29101 caacgtgcca aaactggcat gtaaaacctt cctgcgtgat tacaccgacg gtatgaaggt
    29161 tgaagcgtta gctaacttcc cgattgaacg cgatctggtg gtcgatatga cccacttcat
    29221 cgaaagtctg gaagcgatca aaccgtacat catcggcaac tcccgcaccg cggatcaggg
    29281 tactaacatc cagaccccgg cgcagatggc aaagtatcac cagttctccg gttgcatcaa
    29341 ctgtggtctg tgctacgccg cgtgcccgca gtttggcctg aacccagagt tcatcggtcc
    29401 ggctgccatt acgctggcgc atcgttataa cgaagatagc cgcgaccacg gtaagaagga
    29461 gcgtatggcg cagttgaaca gccagaacgg cgtatggagc tgtactttcg tgggctattg
    29521 ctccgaagtc tgcccgaaac acgtcgatcc ggctgcggcc attcagcagg gcaaagtaga
    29581 aagttcgaaa gactttctta tcgcgaccct gaaaccacgc taaggagtgc aacatgacga
    29641 ctaaacgtaa accgtatgta cggccaatga cgtccacctg gtggaaaaaa ttgccgtttt
    29701 atcgctttta catgctgcgc gaaggcacgg cggttccggc tgtgtggttc agcattgaac
    29761 tgattttcgg gctgtttgcc ctgaaaaatg gcccggaagc ctgggcggga ttcgtcgact
    29821 ttttacaaaa cccggttatc gtgatcatta acctgatcac tctggcggca gctctgctgc
    29881 acaccaaaac ctggtttgaa ctggcaccga aagcggccaa tatcattgta aaagacgaaa
    29941 aaatgggacc agagccaatt atcaaaagtc tctgggcggt aactgtggtt gccaccatcg
    30001 taatcctgtt tgttgccctg tactggtaag gagcctgaga tgattaatcc aaatccaaag
    30061 cgttctgacg aaccggtatt ctggggcctc ttcggggccg gtggtatgtg gagcgcaatt
    30121 attgcgccgg tgatgatcct gctggtgggt attctgctgc cactggggct gtttccgggt
    30181 gatgcgctga gctacgagcg cgttctggcg ttcgcgcaga gcttcattgg tcgcgtattc
    30241 ctgttcctga tgatcgttct gccgctgtgg tgtggtttac accgtatgca ccacgcgatg
    30301 cacgatctga aaattcacgt gcctgcgggc aaatgggttt tctacggtct ggctgctatc
    30361 ctgacagttg tcacgctgat tggtatcgtt acaatctaac
//
LOCUS       DAZFXT010000052        30257 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.052, whole genome shotgun sequence.
ACCESSION   DAZFXT010000052 DAZFXT010000000
VERSION     DAZFXT010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30257
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.052"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKW0_002483"
     CDS             complement(11..259)
                     /gene="hybG"
                     /locus_tag="ACHKW0_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HFO7475301.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKW0_002484"
     CDS             complement(272..613)
                     /gene="hypA"
                     /locus_tag="ACHKW0_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HFO7475302.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKW0_002485"
     CDS             complement(606..1094)
                     /gene="hybE"
                     /locus_tag="ACHKW0_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HFO7475303.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(1087..1581)
                     /locus_tag="ACHKW0_002486"
     CDS             complement(1087..1581)
                     /locus_tag="ACHKW0_002486"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HFO7475304.1"
                     /translation="MRILVLGGGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKW0_002487"
     CDS             complement(1581..3284)
                     /gene="hybC"
                     /locus_tag="ACHKW0_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008901 - ferredoxin hydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HFO7475305.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKW0_002488"
     CDS             complement(3281..4459)
                     /gene="hybB"
                     /locus_tag="ACHKW0_002488"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HFO7475306.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKW0_002489"
     CDS             complement(4449..5435)
                     /gene="hybA"
                     /locus_tag="ACHKW0_002489"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HFO7475307.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSDTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKW0_002490"
     CDS             complement(5438..6556)
                     /gene="hybO"
                     /locus_tag="ACHKW0_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HFO7475308.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKW0_002491"
     CDS             complement(6745..7032)
                     /gene="yghW"
                     /locus_tag="ACHKW0_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 family protein YghW"
                     /protein_id="HFO7475309.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKW0_002492"
     CDS             complement(7151..8038)
                     /gene="yghX"
                     /locus_tag="ACHKW0_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YghX family hydrolase"
                     /protein_id="HFO7475310.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAVGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKW0_002493"
     CDS             8195..9235
                     /gene="gpr"
                     /locus_tag="ACHKW0_002493"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HFO7475311.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(9275..9769)
                     /locus_tag="ACHKW0_002494"
     CDS             complement(9275..9769)
                     /locus_tag="ACHKW0_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HFO7475312.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKW0_002495"
     CDS             9960..10844
                     /gene="yghA"
                     /locus_tag="ACHKW0_002495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7475313.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQQTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKW0_002496"
     CDS             complement(11116..11541)
                     /gene="exbD"
                     /locus_tag="ACHKW0_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HFO7475314.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKW0_002497"
     CDS             complement(11548..12282)
                     /gene="exbB"
                     /locus_tag="ACHKW0_002497"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HFO7475315.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            12534..13721
                     /gene="metC"
                     /locus_tag="ACHKW0_002498"
     CDS             12534..13721
                     /gene="metC"
                     /locus_tag="ACHKW0_002498"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0047804 - cysteine-S-conjugate beta-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HFO7475316.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKW0_002499"
     CDS             13861..14520
                     /gene="yghB"
                     /locus_tag="ACHKW0_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YghB"
                     /protein_id="HFO7475317.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKW0_002500"
     CDS             complement(14560..15459)
                     /gene="yqhC"
                     /locus_tag="ACHKW0_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HFO7475318.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQSSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKW0_002501"
     CDS             15653..16816
                     /gene="yqhD"
                     /locus_tag="ACHKW0_002501"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1990362 - butanol dehydrogenase (NAD+)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HFO7475319.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAETRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKW0_002502"
     CDS             16921..17748
                     /gene="dkgA"
                     /locus_tag="ACHKW0_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050580 - 2,5-didehydrogluconate reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HFO7475320.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIVENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            17951..18877
                     /locus_tag="ACHKW0_002503"
     CDS             17951..18877
                     /locus_tag="ACHKW0_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HFO7475321.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGEIGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            18928..19185
                     /gene="yqhH"
                     /locus_tag="ACHKW0_002504"
     CDS             18928..19185
                     /gene="yqhH"
                     /locus_tag="ACHKW0_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HFO7475322.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(19228..21447)
                     /locus_tag="ACHKW0_002505"
     CDS             complement(19228..21447)
                     /locus_tag="ACHKW0_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HFO7475323.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(21558..22970)
                     /gene="ftsP"
                     /locus_tag="ACHKW0_002506"
     CDS             complement(21558..22970)
                     /gene="ftsP"
                     /locus_tag="ACHKW0_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HFO7475324.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(23045..23782)
                     /gene="plsC"
                     /locus_tag="ACHKW0_002507"
                     /gene_synonym="parF"
     CDS             complement(23045..23782)
                     /gene="plsC"
                     /locus_tag="ACHKW0_002507"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HFO7475325.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(24015..26273)
                     /gene="parC"
                     /locus_tag="ACHKW0_002508"
     CDS             complement(24015..26273)
                     /gene="parC"
                     /locus_tag="ACHKW0_002508"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HFO7475326.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(26819..27301)
                     /locus_tag="ACHKW0_002509"
     CDS             complement(26819..27301)
                     /locus_tag="ACHKW0_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HFO7475327.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(27354..27746)
                     /gene="ygiW"
                     /locus_tag="ACHKW0_002510"
     CDS             complement(27354..27746)
                     /gene="ygiW"
                     /locus_tag="ACHKW0_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HFO7475328.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            27898..28557
                     /gene="qseB"
                     /locus_tag="ACHKW0_002511"
     CDS             27898..28557
                     /gene="qseB"
                     /locus_tag="ACHKW0_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing response regulator transcription
                     factor QseB"
                     /protein_id="HFO7475329.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            28554..29903
                     /gene="qseC"
                     /locus_tag="ACHKW0_002512"
     CDS             28554..29903
                     /gene="qseC"
                     /locus_tag="ACHKW0_002512"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quorum sensing histidine kinase QseC"
                     /protein_id="HFO7475330.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(29949..>30257)
                     /locus_tag="ACHKW0_002513"
     CDS             complement(29949..>30257)
                     /locus_tag="ACHKW0_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HFO7475331.1"
                     /translation="IFEALLYIMLTYWLIDAFFAFNKYDWMLESGDNICSIPSVSGED
                     RILQAMIAAFFLLTPLIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY
                     "
BASE COUNT         7466 a         8081 c         7643 g         7067 t
ORIGIN      
        1 aagctaatca tcacgcgctg gtaatgtcgt aatccatttg gcgcagtgcg tctaatgtgg
       61 ctttggcttc atcttcgtcg atgatgctca tggcaaatcc gacgtgtacc agcacccact
      121 ggcccagcag atcggcaggg ttaccttcac aaatcagggc gatattcacg tcgcgcttga
      181 taccacatac ttcaacctgc gcaagctggt gaatatcttc accgacagcc agcacctggc
      241 ctggaacgcc aatacacatt attaactccg gttattcaac ttcaatactt ttgacgatca
      301 gcgaatcgcc ggtatcgaca cgcaaccgct cgccatgaca gatcggacac tgcgcatcgt
      361 gctgatgaat ctccaccacc tggctgcaat cccagcacca ggcctgggcg ggtttataga
      421 cgatatgtaa atcgcaccct tgcgccaccg ttccgtggca gacaatttca aaactaaaac
      481 ggacggcgct ctcctcaacg caggagagcg cgccaatttc cagccacacg gcggtgacgc
      541 gcttaacatc gtgctgctcc gcctgccgtt ggataatttc aacggcgctc tggcaaagcg
      601 acaactcatg cattttcgcc actcctgcga ccaaacagca gggcgcgacg ccctgcgtgt
      661 ggtacatccg gattcgtgac tggcagcgaa aggatcattc gtgcgcagtc atccgtcagg
      721 cgttggccct cttcaatcga catactgtgc gaaagcggtg acatcagcga acaggagagg
      781 tattgcgaaa caccgtccag ttcgccaacg gtaaaggtca tggtgccata cggcagttgc
      841 agaccaattt tttcactcac tttgcgcagc ggccagagtt gatccgggcc ggggaagata
      901 actgcactca gcatccacgg ggtgatcaca caccccgtcc actgaccttc gaacagcgta
      961 aaatcagaaa catacaccgg cattgaagga tgcagaaaag agagatcgtg catcgaacgc
     1021 cgggcaattt cttcaaacgc tgcctgtact tgcgccttcg gggaggtctg gaaacctgct
     1081 atctcttcag tcatgaatcg cctcccgtgg gatggcttcc acgcctgatt cgcgcagcgc
     1141 agccagaacc tgctcaagcg caggttcaat cattgcttca accgtcggcg ttaagccgat
     1201 gtgtggctcc agcgattccg ggatcacgcc gaccagggtc agctttttcg gaaactcgcc
     1261 ggtgaagcgc agggccgaca agacgtcggc caggccaagc tgatgcggag agattttgtt
     1321 ggtaaacaac gccggaactt cttcatcccg caggatcatc atcgttcccg gcgcgctctt
     1381 cttcgacaca atggcatccg caataatcag atgatcgcga tttgccatgt cgccaagcag
     1441 ctccattccc gccgtgccgc catcgaggat ctcaacataa tccggcagaa tgtatcgttg
     1501 ctctaacgct tcaacaatcc gcacaccgat ggcttcatcg gtcagcaaaa tattgccgcc
     1561 ccctaagact aaaatacgca ttacagaacc ttcactgaaa ccacttcgtt gccgtcagca
     1621 tccactacgt gtaccgcaca ggccatgcac gggtcgaagg agtgaatggt acgcaccact
     1681 tccagcggtt tattcggatc agcaaccggt gtacccacca gcgactgctc gtaaggaccg
     1741 acgtcatcat tgaagttacg cggaccagag ttccaggttg atggaacaac tgcctggtag
     1801 ttgctgatga taccgtcttt gatcaccatc cagtgagaga gcataccgcg cggcgcttcg
     1861 aggaagccaa cacctttgaa ctcacccgtt gccggaatgt tcggtttcac aaaggtggta
     1921 tgatcgcctt tgccgatatt ggtgatcagt gcactgtatt ggttttgcag gatatcctgc
     1981 aattcgcagc agtgaacggt acgaccaata atacggccca gcgtagagtg cagttgcgcc
     2041 acttccagcg tgttaccagt cagtttctga taaatcgcaa cgatttcatt cagtttgttc
     2101 tgggtagatt ctcgacctgc cgccagtttc accagcatat tagccagcgg acccacttct
     2161 accgttttgc cgtagaaagt cggtgatttc acccaggaat atttcccgtc gtcagaccaa
     2221 ccatcataag ccggaatggt ggtaccttcc cacggcgcct gtggcgcttc gtctttatac
     2281 caggagtgct tcgcgctttc ctgaatgcct ttgatcaggt attcatcgga atgagaagtg
     2341 atcggacgat acgaggacag atccgcattc tcaatgtagc cgcccgggaa caggaagctg
     2401 ccgtttttgc tgtcggtcgg gaattccggc acgctcaggt agttcaccgc acctttaccg
     2461 cgcgtcagcc attccgggta gaacgcggcg ataaccgcgg tatcaacctt ataaacctgc
     2521 tcaacaaagt cgctcagttt gtcgatgaaa gacttgatgt acatcaggcg ctcaaggttc
     2581 aacacgccca acccgtcgag gttgattggg ttcgcgacac cacccactgc caggttctga
     2641 atgtgcggcg ttttaccgcc cagcagcgcc acgacgcggt tagcgtcacg ctggcactcc
     2701 agcgcctgca ggtagtgcgc taccgcaatc aggttcactt ccggcggcag tttcatcgct
     2761 gggtgacccc agtagccatt agcgaaaata cccaactgac cgctggcaac cagatctttg
     2821 atcttgttct gaactttggt gaactcttcc gggctgttca gatgccaggt cgaaacgcct
     2881 ttcagcattt cggaggcttt ggttgggtca gcttgcagtg cagaagtgat atccacccag
     2941 tccagcgccg aaagctgata gaaatgaacg atatggtcat gcgtggtgtg cgcagccaga
     3001 atgatgttac ggatgtattg cgcgttaacc ggaacgtcga tattcagcgc actttctgcc
     3061 gcacgaacgg aagacagcgc gtgagtggta gtacatacgc cacagatacg ttgcacaatc
     3121 atccatgcat cgcgcggatc gcggtttttc acgatctctt ccatgccgcg ccacatggta
     3181 ccggaagccc atgctttcga aacgacgcca ttttcgattt cgcaatcgat gcgtaaatgc
     3241 ccctcaatac gggttaccgg atcaatagta attctctggc tcatgctttg ctcgcctcat
     3301 gacgattatg atcgttttgt tttaaaggag gaagtatcgg cagtagacga atgagtacga
     3361 tgtaagcgca aatctcaata gccacaaaac caatagaaat caacagttct tcccaggtcg
     3421 ggaagtaggc gtaaccgccg cccgggttga atgccaccag cgaataggtc agacgccagg
     3481 ttgcacaacc taacagtgcg ctcagtgctg acaggaacag catgcgggaa tcattacgca
     3541 gcttcgccac acgcagaacc accagcggga agagcatcag caggacttca atccagaaca
     3601 tcacggagta gaagtcaccg gcaaacgcta acgacagctt gtcgcgatag ataagctcgc
     3661 caaagcgcag cacgatgaaa atcgccagca acacactgat ggtgttggtc agcttgacga
     3721 acagactctt ttcatccgga ccgttgccac gcagacccgc ctgcaccagc gaaccttcaa
     3781 agatgacaat cgagaagccc atgatgaacg ccgtcagcag cgagaacagc ggcaacattt
     3841 catagctctg ccacaacgga tgcaccttgt agcccgccga gatcatcagt gaccccattg
     3901 aagactggtg catggtcggc aacagcgcgc caagtgcaat gatgaagaac atcaccttgt
     3961 tcagacgctt gagagaaacc ttccagccca gacgttcaaa cagtgccgga gcaaactcca
     4021 gtgccatcac gccgatatag atggtcatac agaccgccgt ctcgaacagt accgagttca
     4081 cgttgaagtg acccggaatg tagaagtacg gcaggttcca gtagcgaccc acgtcgatag
     4141 tgatcgacaa gccccccagt gagtaaccaa acagactcgc caacagcgcc ggacgcacca
     4201 gcggatggta ttgcccacgg ttaaagacgt ataccgccca cgccagcgcc cagccgccac
     4261 aggcaaagcc ggtaccaatc agcaggtcaa acgcgatcca cacgccccac gggaagccgc
     4321 cgttcaggtc agagacagag cccagaccga acaccagacg cttcacaatc aggagcatac
     4381 agatgacgat taacggtcca aaaatcatga ccggtttact gatgattttg ccgcccagcg
     4441 gttgtggatc atgactcatg atcgtctcct ccgtcgtgat ggtcgttttt ggtgttgcga
     4501 cgaaccagca cggttaagcc cgccagcaca gccagtggta gcatcatgcc tttatacagg
     4561 gtgtgttgaa tattttcgga acgcgcaccg gtagaaagat cgtccagttt cggcaggtcg
     4621 agattttcat aaggcacacc cgtcagtacc agaacctgag taccgccgcc ctctttctcg
     4681 ccgtacagat gcggataata tttcggcacc gtatgcaggt aagtgtcgcc agatttcagc
     4741 gtctgacgcg gatagtggta ttcgctgcca ggcttcagcg ccagacgttt tttcgcctcc
     4801 gccatcagct cttcacgcgt accaaaaatt accgcgcccg ccgggcacac ttctacgcag
     4861 ccaggcaaac cgcctttatc gagacgttcc acacctttct ggttgcacaa ctcgcactta
     4921 tgcagcgcac caaacgggtt gttgtagtcg tacttcggca cgttgtatgg gcaggcgacc
     4981 atgcagtaac ggcaaccggt gcacacatct ttgtcgtaat ggacaatgcc ggttttcgga
     5041 tcttttttca gcgcagagac cgggcacaca gagacacagt tcggatcgac gcagtgcata
     5101 cactgtttct taatgtacgc gtagccgttc tcctcctggt ctttgttgac ccctgtgtcg
     5161 ctggtccaca cctgaatgat gttattggta tacggcgaca gtttgtcgtt gttcgaccag
     5221 gtctgttccc cttgcgggtt acgttcaggg aagttgatat cctgacactt ggtgacgcaa
     5281 gcctggcagc ctacgcacaa ggtcgagtcg tacaacatcc ccagcgatcc cggaattggc
     5341 gggcggtttt cagcagccgc atgactgaca gacggcagcg cgcccgtcag caatgccccg
     5401 caggaggctg ctttaataaa attacgtctg ttcacggtta ttctccccgt gagtcagcgt
     5461 tatctttctt ttgctgacga cccagttcac gcaccgccat cacgctgaca ccggcaacca
     5521 gcccaaccac accgccgagc aaaccaatag cgcctgcaga gacgttgccg ccctctttag
     5581 cgttaacatc cggtttctgt gaacgcggag tctgattttc gacgttggca agctgatgga
     5641 tgcctttatg gaagccgata ccttcttcgt tacagccata gcatgggtga ccaatcgcta
     5701 ccggccacac gccgccaaca tcgcagaatt gcagcgttga gcagttgccg taagtttctg
     5761 gccctttaca gccgaggtgg tacaggcacc agccttcacg gtggccttca tcaccgaact
     5821 ctttggcaaa acgaccagca tcgaagtgcg ggcgacgttc gcagtgttcg tgaatcagac
     5881 ggccataggc gaaggtcgga cgatttttgt catccagttt cggcggtttg ccgtaagtga
     5941 tgatgtgcgc aacggtcgcg aggaagttgt gcgggttcgg cgggcagccc ggaatattga
     6001 taacggtttt gcctggcaga acttcttgca ggctgactgc gccagttggg ttaactccag
     6061 ctgcggcaac accgccccac gcagagcagg aaccgatagc gataatcgct gctgcgcctt
     6121 ctgccgcttt gcggatgtga tccacaatcg gctcaccagc aaccatgcaa taaataccgt
     6181 tatcttttaa tgggatggaa ccatccacaa ccaacacata ctgccctttg tacttctcaa
     6241 gtgcgttatg tttgttctct tcgacctgat gaccgaaggc ggcggaaagc acttcgtgat
     6301 actccagaga gatagtctcc agcacgaggt tttctaccgt tggatgcgtt gcacgaagca
     6361 gagattccgt acaaccggtg cactcctgcg cgccaatcca gataactggc ggacgctgcg
     6421 ggttagtaac cgattcggcc atctctgcag cggctttgct acttaacccc atggtggcgg
     6481 ctaatgctgc acaaagcttc atgaaatcac gacggttaat gccgtgagaa tggatgaggg
     6541 tgttatctcc agtcatttat agttattccg ttgcgaagac ctggcatata ttttgcctca
     6601 atcgcaaaat caataatgcg atcgatgcgc catttaccac acatttatta tggttatcga
     6661 tatcatgata ctgcggcggg gcgctaaaac gaagggaaga agcattagtg taattaatta
     6721 aagcaagata atacgtatgt ttgatcaatt ttcgaggcga taaccggcca taaaaaagcg
     6781 caacgtagaa caggaattat tctcacgaaa gaaatccatt accatctctt tatccagtcc
     6841 atagcggcgc gtcagaatac ccgcttccca ggcgctaagc tggcgatctc cggtcttttc
     6901 gtacatccgg tgtgagtagc ctaatacaaa accgcgttta taatcggcac agaattttgt
     6961 cacattaacc gcactgtcgg catgcgttgc ttttaatccc gccattaagc ctttaccaaa
     7021 atggttattc atcgttcacc tcattcgcta tatatcgcca tatatagcga tattttaggc
     7081 aaagaacgac ttttgcgcac gctatttttt catgcttgtt taagacgtaa tccagtaaga
     7141 gataaaccta tcaggagaga tatttatcga accatttcag tgtcctttgc caggcaagat
     7201 cggcggcaga tttgtcataa cggggcgtgg aatcattatg gaatccgtga ttaacccccg
     7261 gatagatata cgcctcataa accttattat tggctttcaa cgccgcctcg taagcaggcc
     7321 agccctcgtt gattcgggtg tccagttccg cgaagtggag tagtaaaggc gcttcaatct
     7381 tcgccacatc ggcagtgggt gcctgacgac cataaaacgg caccgcgcag gccagttccg
     7441 gatacgcgac agccgccgcg ttcgatacgc caccgccata gcaaaatccg gtaataccca
     7501 ctttgcctac cgcttgcgga tagcgttgca taaactcaat tgcggcaaag aaatcattca
     7561 tcagtttggt tggatcaacc tgctgttgca gctcacgacc tttatcatca tttcccggat
     7621 aacctccaac ggaacttaag ccgtcaggtg ccagggcgat ataccccgcc ttcgccactc
     7681 gccgtgccac atcttcgata tacggattca gtccacgatt ctcatgcacc actaccacgg
     7741 ctggcgtttt gccgctcatt tttgcgggtt tcaccagata accccgtacc tcgccgtgac
     7801 catttggcga aggatacgtg atgtactcag caacaatctc cgggtcggta aactctacct
     7861 gagtcgccag cgcataattt ggcttgagca aatcaaacaa cgctaatgcc gtcatcccgc
     7921 ccaccgcata cttcgccgca aggttgagga actcacgttt cgagattttc ccgtgagcgt
     7981 aatagtcgta gtaatccaac aactcttgtg ggaaatcttt ggcggttaaa cgcggcatcg
     8041 ttgcactcct caactggtgt ttttttaagc aaagcataag cacgtgtttt tgcccagttt
     8101 ttcgtcactc tgtgagccag actacgggat acgcgctggc gaatcgctaa actagaaaca
     8161 ttgtttcgaa attgaacggt ggaaaggaga ggtcatggtc tggttagcga atcccgaacg
     8221 ttacgggcag atgcaatacc gctattgcgg aaaaagcggt ttacgcctgc ccgcgttatc
     8281 gctcggttta tggcacaatt tcggtcacgt taacgcgctg gaatcacagc gtgcgatcct
     8341 gcgtaaagcg tttgatttgg gcattacgca ctttgattta gccaacaatt acgggccgcc
     8401 tccaggaagc gcagaagaga actttggtcg cctgctgcgg gaggattttg ccgcttatcg
     8461 cgatgaactg attatctcta ccaaggctgg ctacgatatg tggcccggcc cttacggctc
     8521 tggcggttca cgtaaatacc tgctcgccag cctcgaccaa agcctgaagc gtatggggct
     8581 tgagtatgtc gatatctttt actctcatcg cgtcgatgaa aatacgccga tggaagaaac
     8641 cgcctctgcg ctggctcatg cggtacaaag cggtaaggcg ctgtatgtcg ggatctcctc
     8701 ttactcgcca gagcggacgc aaaaaatggt cgagttgctg cacgagtgga aaattccgct
     8761 tttaattcat caaccttcgt acaatttact gaaccgctgg gtggataaaa gcggcctgct
     8821 ggataccctg caaaataacg gcgtgggctg tattgccttt actcctctgg ctcagggatt
     8881 gctgaccgga aaatatctca acggcattcc gcaagattca cggatgtatc gtgaagggaa
     8941 taaagttcgt ggtctgacac cgaaaatgct taccgaagcc aacctcaaca gcctgcgctt
     9001 attgaatgaa atggcacagc agcgtggaca atcaatggcg caaatggcgt taagttggtt
     9061 gctgaaagat gagcgagtga cgtcggtatt gattggtgcc agccgcgcgg agcaacttga
     9121 ggagaacgtg caggcgctga ataatctgac atttagcacc gaggagctgg cgcagattga
     9181 tcagcatatc gccgatggcg agctgaatct gtggcaggcg tcgtccgata aatgacctgt
     9241 taataacggg cgcgcgaacc gcgcccgaat cagatcagtg attatgacga gtcagtcggt
     9301 caagatagcc catcacaaat gcagaaagca caaacgtcag atggataatg acgtaccaca
     9361 tcagtttgtt atccgggaca tttttcgcat ccataaagac gcgcagtagg tgaatggaag
     9421 aaattgccac aatcgacgct gctactttgt ttttcagcga cgttgcgtcc attttcccca
     9481 gccagttcag cttctctttg ttctcggaga tatccagctg tgagacgaaa ttctcataac
     9541 cggaaaacat caccatcacc agcaaaccgc caaccagcgt catatccacc agcgacagca
     9601 acacgaggat caaatctgat tccgccatcg agaagatatt cggcagtacg tgaataatct
     9661 cctggaagaa cttcagcgcc agggcaacta acgccagcga aaggccaaag tacacggggg
     9721 caagcagcca gcgagaagca tacattgcat tttcaagaaa acgttccata cattcctgtc
     9781 gtgtagttaa aacgggcaac agtatatcgc aattgagtaa acagcacgca acagcaaatc
     9841 aacatatacg ttaaaccttt gtcgcataat tctgaggttt agggatcttc ctggttccct
     9901 tatcaacgca tcgagagcgt ctacccttaa aggacgttga acctgaaggg agaaaaacga
     9961 tgtctcattt aaaagacccg accacgcagt attacactgg tgaatatccc aaacagaaac
    10021 aaccgacgcc aggcatccag gcgaagatga caccggtacc ggattgcggc gagaaaacct
    10081 atgttggtag cggtcgcctg aaagatcgta aagcactggt gacagggggc gattccggaa
    10141 taggtcgcgc tgccgccatc gcttacgcgc gtgaaggggc tgacgtggcg atcagttatc
    10201 ttcccgtgga agaagaagac gctcaggatg tgaaaaagat cattgaagaa tgcggacgca
    10261 aagccgttct gctgccaggc gatttaagcg atgagaaatt cgcccgttcg ctggttcacg
    10321 aagcgcataa ggcgttaggc gggctggata ttatggcgct ggtcgccggg aaacaggttg
    10381 ccattccgga tattgcagac ctcaccagcg aacagtttca aaagaccttt gccattaacg
    10441 ttttcgcgct gttctggcta acccaggaag cgatccccct gctaccgaaa ggtgcaagta
    10501 tcatcaccac ttcgtcaatc caggcatacc agccaagtcc gcatttactg gactatgcgg
    10561 ctacgaaggc ggcgattctg aactacagcc gtggcctggc aaaacaggtc gcggagaaag
    10621 gtattcgggt gaatattgtc gcgccaggcc cgatctggac agcactgcaa atttccggcg
    10681 gacaaacgca ggataagatc ccgcagtttg gtcagcaaac gccgatgaag cgtgcggggc
    10741 aaccggcgga actggcccct gtatatgttt atctggcaag tcaggagtcg agctacgtca
    10801 ccgcagaagt gcacggtgtg tgcggcggcg agcatttagg ttaaaaaaat gcccggttgt
    10861 gaaaagcaac cgggcatcat tgtgaattca ctcttacctg atgtggcgaa aacgccttat
    10921 ccgcctacaa acgcatgaag attcaacatg ttgcaataga ttttgtaggc ctgataagcg
    10981 tagcgcatca ggcattgagc acctaatgcc ggatgcgtcg tgaacgcctt atccggccta
    11041 caaacgcacg aagattcaac ctgttgcaat agattttgta ggcctgatga gcgtagcgca
    11101 tcaggcaatt ctactttact tcgctttggc ggtttcttcg ccgaccagac ctatcttcag
    11161 gtaacccgcc tgatgcagcg tatccattac cttcatcaac gtctcgtaat cgacggtttt
    11221 gtccgctcgg aagaagatgg tggtgtcttt cttgccttcg gttaacgcat tcaacgccgt
    11281 aatcattgtt tcatcggtga ccggatcgtt accgataaac atcgagttgt ctgccttcac
    11341 cgacagataa accggttttt ccgggcgcgg ctgcggcgtg ctggtagaag caggcaagtt
    11401 caccttcaca tctaccgtcg ctaacggtgc cgccaccata aagataatca gcagaaccaa
    11461 catcacgtcg ataaacggcg tcacgttgat atcatgcatt tcgccgttat cgtcgaggtt
    11521 ttcgttaaga tgcattgcca tcggatatta tcctgcgcgt aatttttgtg cgacacgaac
    11581 cggatgcgca gcggcgctgg cttccagatc caggtcacgg ctttgcagca acaatacctg
    11641 cgctgcaaca tcacccagca tcgctttaaa gccgccaatc tggcgtgcaa atacgttata
    11701 gataacgacc gcaggaatcg ctgccacgag gccgattgcc gttgctaaca gagcttctgc
    11761 gatacccggc gcaacgactg ccaggttagt ggtctgcgtt tgcgcgatac caataaagct
    11821 gttcatgatg ccccagaccg taccaaacag accaacgaac ggagaaatcg cgccgatggt
    11881 tgccagatag ccgttaccgc ggcccatttg acgacccact gcggcgaccc gacgctccag
    11941 acggaagctg gtacgttctt taataccttc gttatcgtcg ctgccttctg acagttccag
    12001 ctcattctgc gcttcattga gcaaatgcag gcttaagctt ttgctaccaa aatcagcggc
    12061 gatatcgttg gcctggttta aggaacgcgc ttcagccagc agttgctgct cgcgcttaag
    12121 gcgacgcttc tgattgaaga actctacgct cttactgaag aagattgccc aggtgactac
    12181 ggaggccaaa ataagcccaa tcatcacgca cttaacgaca atatcggcgt gctgatacat
    12241 accccagacg gaaaggtccg tctgcattaa attattaccc actgtgtatc tccaggacgc
    12301 aagtcacaaa atctgcgcat aataatatca aaacgacgtc gaattgatag tcgttctcat
    12361 tactatttgc atactgccgt acctttgctt tcttttcctt gcgtttacgc agtaaaaaag
    12421 tcaccagcac gccatttgcg aaaattttct gctttatgcc aattcttcag gatgcgcccg
    12481 cgaatattca tgctagttta gacatccaga cgtataaaaa caggaatccc gagatggcgg
    12541 acaaaaagct tgatactcaa ctggtgaatg caggacgcag caaaaaatac actctcggcg
    12601 cggtaaatag cgtgattcag cgcgcttctt cgctggtctt tgacagtgtg gaagccaaaa
    12661 agcacgcgac gcgcaatcgc gccaatggcg agttgttcta tggacggcgc ggaacgttaa
    12721 cccatttctc cttacaacaa gcgatgtgtg aactggaagg tggcgcaggc tgcgtgctat
    12781 ttccctgcgg ggcggcagcg gttgctaatt ccattcttgc ttttgtcgaa cagggcgatc
    12841 atgtgttgat gaccaacacc gcctatgaac cgagtcagga tttctgtagc aaaatcctca
    12901 gcaaactggg cgtaacgaca tcgtggtttg atccgctgat tggtgccgat atcgttaagc
    12961 atctgcagcc aaacactaaa atcgtgtttc tggaatcgcc aggctccatc accatggaag
    13021 tccacgacgt tccggcgatt gttgccgccg tgcgcagtgt ggtcccggat gccatcatta
    13081 tgatcgacaa cacctgggca gccggtgtgc tgtttaaggc gctggatttt ggcatcgatg
    13141 tttccattca ggccgctacc aaatatctgg ttgggcattc agatgcgatg attggcactg
    13201 ccgtgtgcaa tgcccgttgc tgggagcagc tgcgggagaa tgcctatctg atgggccaga
    13261 tggtcgatgc cgataccgcc tatataacca gccgtggcct gcgcacatta ggtgtgcgtt
    13321 tgcgtcaaca tcatgaaagc agtctgaaag tggctgaatg gctggcagaa catccgcaag
    13381 ttgcgcgagt taaccaccct gctctgcctg gcagtaaagg ccacgaattc tggaaacgag
    13441 actttacagg cagcagcggg ctattttcct ttgtgcttaa gaaaaaactc agtaatgaag
    13501 agctggcgaa ctatctggat aacttcagtt tattcagcat ggcctactcg tggggcgggt
    13561 atgaatcgtt gatcctggcg aatcaaccag aacatatcgc cgccattcgc ccacaaggcg
    13621 agatcgattt tagcgggacc ttgattcgcc tgcatattgg tctggaagat gtcgacgatc
    13681 tgattgccga tctggacgcc ggttttgcgc gaattgtata acattgccac ttttggacaa
    13741 ttttgcagac atttaattgt gaaaagtctt aaattgttgc gtccgggatc aaggcgtccc
    13801 ggacgaatca ggagtacaat aggcagataa aggcttaaac gctgttccac aggaaagtcc
    13861 atggctgtta ttcaagatat catcgctgcg ctctggcaac acgactttgc cgcgctggcg
    13921 gatcctcata ttgttagcgt tgtttacttt gtcatgtttg ccacgctgtt tttagaaaac
    13981 ggcctgctgc ccgcctcatt tttgccaggc gacagcttgt tgatactggc aggcgcattg
    14041 attgcccaag gggttatgga ttttctgcct acgattgcga ttctgaccgc cgcagcaagt
    14101 ctgggctgct ggctaagtta tattcagggg cgctggttag ggaataccaa aacggtgaaa
    14161 ggctggctgg cacagcttcc tgctaaatat caccagcgcg ccacctgcat gtttgaccgc
    14221 cacggtctgc tggcgctgct ggctggacgt tttcttgcat ttgtccgtac gctgctgcca
    14281 accatggcgg gaatttccgg tctgccaaac cgccgcttcc agtttttcaa ctggttaagt
    14341 ggattgctgt gggtcagcgt ggtaaccagt tttggctatg ccttaagtat gattccgttc
    14401 gttaaacgcc atgaagatca ggtaatgacg ttcctgatga tcctgccaat tgccttgtta
    14461 accgctggct tgttaggcac gctgtttgtg gtgattaaaa aaaaatactg taacgcctga
    14521 cgattttccc cgttcccggc tgctgtaccg ggaacgtatt taattcccct gcatcgcccg
    14581 cattcttgcc gcatcttccc ccggcgtcac accgaagtaa cgtttaaact cacggctaaa
    14641 ttgcgatgcg ctttcatagc cgacgcgcat cgctgctgcg ctagccttca tgccgtcatg
    14701 gatgatcatc atccgcgcct tatgcagacg gtaattcttc aaatactgca acggcgaggt
    14761 gctggtgaca gacttaaaat tatggtggaa cgccgatacg ctcatgttgg cttctgccgc
    14821 cagttgctcg acgctcaggt tttcggtgta tttattctca atccgtttca gcacgcggct
    14881 aatcagactg aagtgagtct ggcgactgac cagcgccagt aacgcgccgc cgcaaggtcc
    14941 ggtcagcacg tagtacagaa tttcgcggat gatctgtttg ccgagaatac gcgcatccag
    15001 tggtcgctcc atcacgtcga gtaaccgctc cgccgcgcat aaaatctctt ctgataacgt
    15061 ggcggagtta atcccgctgg ctgccatcga cgactggaaa tgctcatctt cgccaatgtc
    15121 catcaacagt tcctgtaact gcaaaatatc gacattgaga cgcaaccctg ccagcggcac
    15181 ctctgacgtg gcataggttt cgcactcaaa cggcaacggc accgtcagca gcaggtattc
    15241 attggcatca taacgaaaca cgcgttcatt gatataaccg attttatgcc cggaaaagag
    15301 aattatgatg ccaggctcgt acatcaccgg tgtacgtgcg aaaggcgtct cgccatacaa
    15361 caaacgcaca tcgggcaaca gttctgacaa actattttct ttatttttca gtttattaac
    15421 tttatccgcc agcaagcggc aaatctcttc acgtttcata tcgcgtaatt tcttaggaat
    15481 aatgcggcaa tttgattgtg cgcaattttg tagcatttct ccagcactct ggagaaatag
    15541 gcaagacatt ggcagaaatg agcattgaga gccagggcgc tggcgatcac aatgaaaaac
    15601 atcaggcaga tcgttctctg ccctcatatt ggcccagcaa agggagcaag taatgaacaa
    15661 ctttaatctg cacaccccaa cccgcattct gtttggtaaa ggcgcaatcg ctggtttacg
    15721 cgaacaaatt cctcacgatg ctcgcgtatt gattacctac ggtggcggca gcgtgaaaaa
    15781 aaccggcgtt ctcgatcaag ttctggatgc cctgaaaggc atggacgtgc tggaatttgg
    15841 cggtattgag ccaaacccgg cttatgaaac gctgatgaac gccgtgaaac tggttcgcga
    15901 acagaaagtg actttcctgc tggcggttgg cggcggttct gtactggacg gcaccaaatt
    15961 tatcgccgca gcggctaact atccggaaaa tatcgatccg tggcacattc tgcaaacggg
    16021 cggtaaagag attaaaagcg ccatcccgat gggctgtgtg ctgacgctgc cagcaaccgg
    16081 ttcagaatcc aacgcaggcg cggtgatctc ccgtaaaacc acaggcgaca agcaggcgtt
    16141 ccattctgcc catgttcagc cggtatttgc cgtgctcgat ccggtttata cctacaccct
    16201 gccgccgcgt caggtggcta acggcgtagt ggacgccttt gtacacaccg tggaacagta
    16261 tgttaccaaa ccggttgatg ccaaaattca ggaccgtttc gcagaaggca ttttgctgac
    16321 gctaatcgaa gatggtccga aagccctgaa agagccagaa aactacgatg tgcgcgccaa
    16381 cgtcatgtgg gcggcgactc aggcgctgaa cggtttgatt ggcgctggcg taccgcagga
    16441 ctgggcaacg catatgctgg gccacgaact gactgcgatg cacggtctgg atcacgcgca
    16501 aacactggct atcgtcctgc ctgcactgtg gaatgaaaaa cgcgagacca agcgcgctaa
    16561 gctgctgcaa tatgctgaac gcgtctggaa catcactgaa ggttccgatg atgagcgtat
    16621 tgacgccgcg attgccgaaa cccgcaattt ctttgagcaa ttaggcgtgc cgacccacct
    16681 ctccgactac ggtctggacg gcagctccat cccggctttg ctgaaaaaac tggaagagca
    16741 cggcatgacc caactgggcg aaaatcatga cattacgctg gatgtcagcc gccgtatata
    16801 cgaagccgcc cgctaagctt tttacgcctc aaactttcgt tttcgggcat ttcgtccaga
    16861 cttaagttca caacacctca ccggagcctg ctccggtgag ttcatataaa ggaggaacgt
    16921 atggctaatc caaccgttat taagctacag gatggcaatg tcatgcccca gttgggactg
    16981 ggcgtctggc aagcaagtaa tgaggaagta atcaccgcca ttcaaaaagc gttagaagtg
    17041 ggttatcgct cgattgatac cgccgcggcc tataagaacg aagaaggtgt cggcaaagcc
    17101 ctgaaaaatg cctcagtcaa cagagaagaa ctgttcatca ccactaagct gtggaacgac
    17161 gaccacaagc gcccccgcga agccctgctc gacagcctga aaaaactaca gcttgattat
    17221 atcgacctct acttaatgca ctggcccgtt cccgctatcg accattatgt cgaagcatgg
    17281 aaaggcatga tcgaattgca aaaagaggga ttaatcaaaa gcatcggcgt gtgcaacttc
    17341 cagatccatc acctgcaacg cctgattgat gaaactggcg tgacgcctgt gataaaccag
    17401 atcgaacttc acccgctgat gcaacaacgc cagctacatg cctggaacgc gacacacaaa
    17461 atccagaccg aatcctggag cccattagcg caaggaggga aaggcgtttt cgatcagaaa
    17521 gtcattcgcg atctggcaga taaatacggc aaaactccgg cgcagattgt tatccgctgg
    17581 catctggata gcggcctggt ggtgatcccg aaatcggtca caccttcacg cattgtcgaa
    17641 aactttgatg tctgggattt ccgtctcgac aaagacgaac tcggcgaaat tgcaaaactc
    17701 gatcagggca agcgtctcgg tcccgatcct gaccagttcg gcggctaaca tgcaaattct
    17761 cccggtggcg gtaatgttcc gctaccggac ttttcagaaa tcatttattc ccctcgcgtc
    17821 ccgccgcccg ttgttactct tccttgttca ggaatgccaa atataaggac atcatcatgc
    17881 agagccggaa gctcttaaaa gaacaactca tctatatccg ggataaacgc aacggagagg
    17941 tgaaaaacag atgaaaataa tacttctgtt tttagcagcc ctggcaagtt ttaccgtaca
    18001 cgcacagccc ccctcactga ccgtagaaca aacagtccgg catatttatc agaactataa
    18061 atcagatgcc actgcccctt attttggtga aatcggagag cgggcgataa cttctgcgcg
    18121 tattcaacag gcgcttaccc tgaacgacaa tcttacgctg ccgggcaata ttggctggct
    18181 ggattatgat ccggtttgtg attgtcagga ttttggcgat ctggtgctag aaagcgttgc
    18241 gataacccaa actgacgccg atcatgccga tgccgttgtg cgctttcgta tctttaaaga
    18301 tgataaagaa aagaccacgc agacactgaa aatggtggcg gaaaatggtc gttgggtcat
    18361 tgacgatatt gtcagcaatc atggcagcgt cttacaagca gttaatagcg agaatgaaaa
    18421 aacgctggcc gctttagctt cgttgcaaaa agaacagccg gaagcctttg ttgccgaact
    18481 ctttgaacat attgctgatt atagctggcc gtggacgtgg gtggtttccg actcttaccg
    18541 ccaggcggtt aatgccttct ataaaaccac cttcaagacg gccaataatc ccgatgaaga
    18601 tatgcaaata gaacggcaat ttatttacga caatccgatc tgttttggcg aagagtcgct
    18661 attttcacgc gttgatgaaa ttcgagtcct ggagaaaacc gccgattccg cccgtattca
    18721 tgttcgtttt acgctgacca atggcaacaa cgaagagcaa gaactggttt tacagcggcg
    18781 cgaaggcaag tgggaaatcg ctgattttat ccgcccgaac agcggcagcc tacttaagca
    18841 gattgaggca aaaaccgccg ccagattaaa gcaatgagct gaattaaata acaattagcc
    18901 ggaacaataa ataaaaggga acactatatg aaaacgattt tcaccgtggg agctgttgtt
    18961 ctggcaacct gcttgctcag tggctgcgtc aatgagcaaa aggtcaatca gctggcgagc
    19021 aatgtgcaaa cattaaatgc caaaatcgcc cggcttgagc aggatatgaa agcactacgc
    19081 ccacaaatct atgctgccaa atccgaagct aacagagcca atacgcgtct tgatgctcag
    19141 gactattttg attgcctgcg ctgcttgcgt atgtacgcag aatgataaaa aaatccccgg
    19201 cagcatgtca gttgccgggg atttttttta acgtccaacc gccgctttag ggcgtttctt
    19261 cgcaccagca ttcaccggac gagattgcgt agacgacgct ttttttgccg tagcaggcgt
    19321 ctgacgctgg gtcgccatcg gcgtatgttt cgtcaacgcc ggacgggtat tgcggttctg
    19381 gcgacgagct tcacgcatct cttcaatggt tggcgcaggc actaagcaat cgcgacggct
    19441 gccaatcaga tgctttttgc ccatcgcttc cagcgcctgg cggattaacg gccagtttgc
    19501 cggatcgtgg taacgcaaca acgctttatg caaacgacgc tgtttgtcgc ccttcggtac
    19561 gaagacgtct tcgctcttat aaccaatctt cgccagcggg tttttcccgg tgtaatacat
    19621 ggtggttgag ttcgccagcg gcgatgggta gaagttctgt acctggtcga gacggaaacg
    19681 atgctttttc agccacagcg ccagattcac catatcttca tcacgcgtac cggggtgcgc
    19741 ggagatgaaa taggggatca gatactgctc tttacctgcc tgttttgagt aagtatcgaa
    19801 cagctcttta aagcggtcat agctgcccat gcccggcttc atcatcttcg ataacggccc
    19861 ttcttcggta tgttccgggg caatcttcag ataaccgccg acgtgatggg tcgccaactc
    19921 tttgatatag cgcggatctt ccacggcgat gtcataacgt acgcctgagg cgatgaggat
    19981 ttttttaatg cctttcagat cacgcgcacg gcgatagagg ttgatcgtcg gttcgtggtt
    20041 agtgtccatg tgcggacaaa tatccggata aacgcacgac aaacggcgac aagtttgttc
    20101 agcgcgtggc gatttgcagc gcaacatata catgttggca gttggcccac cgagatcgga
    20161 aatcacgccc gtaaaacctg gaacggtgtc gcggatcgct tcgatctcat taatgatcga
    20221 atcttccgaa cggctctgaa taatgcgccc ttcgtgctcg gtaatagaac agaaagaaca
    20281 gccgccaaag cagccgcgca taatgttgac cgaaaaacgg atcatttcgt aagccggaat
    20341 acgggcattg ccataggccg gatgtggcac gcgcttgtac ggcagcgcaa aaacgctgtc
    20401 catctcttcg gtagaaagcg ggatagcagg cgggttgatc cacacatagc ggtcaccgtg
    20461 tttttgcatc aatgcgcggg cgcagcctgg gttggtttcg tggtgcagaa tgcgcgaagc
    20521 atgggcgtac agcactttat cgcccttcac tttctcgaaa gaaggcagca acacgtaggt
    20581 tttttcccac ggtttcgggc gcggtggctg cacggttacg gctttggctt cctgcttttt
    20641 cggtgccacc ggtttgttat ccgcgcacgg caaatcttca ccatacggat gcgggattgg
    20701 gtcgattttt ccaggggtat caagacgggt ggaatccacg ccgctccagc caggcagcgc
    20761 ctctttcacg ataatcgcgg tattacgcac atcgcggatt tcactaatcg gctcgcccat
    20821 cgccagacgg tgcgccacct ccaccagcgg acgctcaccg ttaccaaaca tcagcatgtc
    20881 ggctttcgaa tccaccagca cggaacggcg cacggtatcg gaccagtaat cataatgtgc
    20941 ggtacggcgc agactagcct caataccgcc gaggatcacc ggtacatctt tccacgcctc
    21001 tttacaacgc tgggtataaa ccagtgtggc gcgatccggg cgcttacccg cgacgttatc
    21061 cggcgtgtag gcatcgtcat gacgtaaacg gcgatcggcg gtataacggt tgatcatcga
    21121 atccatgttg ccagcagtaa caccgaaaaa cagattcggt ttacccagac gcatgaaatc
    21181 gtctttgctg ctccagtctg gctgggcgat gatcccgacg cgaaagccct gtgcttccag
    21241 catacgaccg caaatcgcca tcccgaagct tgggtgatcg acatacgcgt cgccagtaac
    21301 caaaatgatg tcgcagctat cccagccaag ttgatccatc tcttcacgag acatcggcaa
    21361 aaacggtgcc ggtccaaaac aggcggccca gtactgcggc caggagaaca ggtcgcgatc
    21421 cggttggatc agggagatag agctcatttt gcttccagaa atgataaaaa aataatcaaa
    21481 ggccggggat tataagccgg aacgaaagag aaatcgaaag gtattccata ctcgccctcc
    21541 tcgggcgagt atgaagatta cggtaccgga ttgaccaaaa gttgccctat cgagccacgg
    21601 tccgccattt ccagcgtctg actgttgaag tagaacggga agtgcgccca ggaaggctga
    21661 ccgaaataaa caagcagctc cacttgtccg tcaacccaaa cggtatcttt ccagcctctg
    21721 tcttccggga acggcatcgc gccattcacg ttacggatct ggaacattac gccttcaata
    21781 tggaacgctt gcggctcgtc cgcgcgtacc gtccagcgtt cccacgttcc ttgctgcgcg
    21841 gtgacatcaa tacggttgac gtcccacagc tgtccattaa tacccgggtc atcacccaga
    21901 ctgatatcgc gactgcgaat tggcgaacca gccatgattt cagttggcag caagcgcatc
    21961 ggaagactgt ctgtgaccag cggcagaagg ccggttgggc gcagcgttag caccagggta
    22021 gaaaccagaa tactggatgg ctcaaagaag ccgcgaatac gatcaacaat gctcgccgct
    22081 tcgccacagg tgatcgacac ttcatcgccg ttgctcatat ccaccagaat ctcgcggcgc
    22141 tcgcccggtg ccagcgaaag ttgcttcacc gatacaggag caggcaggaa tccctgatcg
    22201 ccagaaatca catgtaacgg gcgaccatcg ctcatctgta gttgatagcg acgagagttc
    22261 gacgcgttca gcagtcgcaa gcgcacccag ccacgcgaga cttcaacgta cgggctttgt
    22321 acaccgttaa ccagcagcgt atcaccaaca aagccgccgc ttcccggttc gttgtattct
    22381 ggcgtaccaa agttatccag ccgtttatcc tggataatga ccggaaaatc atccacacca
    22441 taatggttgg ggataggcag cgacttgctg acttcatctt ccaccagcca cattccggca
    22501 aggccgttat agacctgctg agccgtgcgg ttgggagtat tggcgtgata ccacagagta
    22561 gctgcgttct ggcgaatagg cagtacgggt gcccagtcag cgtttggcga catcatccgt
    22621 gccggaccgc ccatcagcgg gcctggtacc tgtagcccgg ccaccgtcat tgagacattt
    22681 tctgtcaggc ggttgctgta aataagctta acatcgtcgc ccttccagac gcggatagtc
    22741 ggccccaggt aacgaccatt gattccccag accgacgcgc gtgtccctgg cgtaaatgac
    22801 caatgcgcac gttgtacagt cataaacagc ggttgcccac ggcgagattc aagtagcggc
    22861 ggaacgggta gcggttgctg ttgcccggct gcgctggcct tcaggggaac agcgcctgca
    22921 caaagtgcaa tccccgatgc ctgaatgaac tgacgccgac tgagtgacat atttgctcca
    22981 tgtaaaactg gctaaagtaa taccgtgcag gaaagtgttc cccgcaaaaa tacagattgc
    23041 cgacttaaac ttttccggcg gcttcgcgtt ctgcgacttc tttatcgagc tcggcgattt
    23101 tttgttccat tatcgaacga caatgggcag ccagctcacg aacctgatct ttgccatact
    23161 gactgacgtc aattggcggc agcatttcga caatcaccag accgttgtgc agtcgattaa
    23221 gattaatctt attcgaagtt gtagagacgc acacgggaat aatcgggacg cccgccgcaa
    23281 ttgccgcgtg aaatgctcca gtcttgaacg gtagcaggcc gcgaccacgg ctgcgggttc
    23341 cttccgggaa catccagatg gaaatacggc gttttttgaa atgattcacg acttccgcaa
    23401 tggtgccgtg agctttagtg cgattgtttc tgtcgatcaa taagttgccg gttaaccagt
    23461 acaactgccc gaagaagggg atccacagca agctcttttt acctaccgtc accgtcggcg
    23521 gttgcacaat gttcgatgct gtcaccatgt catagttgtt ctggtggtta gcgatataga
    23581 tagcattgcc gtagctttca gcatctgcag gtttgcggca ctcaactttc aggccgaaca
    23641 gcggcgcaag acggccaaac atatgcccaa aggtggccac atgtttcggg ttacgcgggc
    23701 tgaaaaggca gtaaatggag ccgaatacac agactaagat gctgtaaatc acggtaataa
    23761 taagacgaaa gatatatagc ataacaccct ctgaaggtct gagagactgg cattgtacgt
    23821 cacctgaaat caggttaggg cattgttaaa agccgctctc tggaaaagcg tattgttaat
    23881 cgcaacgcat gaataaacaa cggtttcaag ggaattttta tcgtttccgg gggttgaata
    23941 cgctgccgga tgacgactta acgtttcatc cggcgttcct tgcaagcggg aggaaacagg
    24001 ccctccccgg catattactc ttcgctatca ccgctgctgg cacggcgagg agagtcgatc
    24061 tcaacacgat cgatacgctg caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca
    24121 gtgactttct gtaactcttc cgggcgcagt ttaattttgc gtttcccaac atgaatggtc
    24181 agcgtgcttt gcggcggcag aacgtacaat tgcgccagac catcttctcc acgcgcggct
    24241 tctgccgatg gaatgttgat aatcttgttg cctttgccct tcgacagctg cggcagatcg
    24301 cttaccggga acatcaacat acggcctgcc tgagtgattg ccagcagcat atcggaagca
    24361 tcttcaatca ccaccggcgg cataacatgg gcattttccg gtaaggtgat caaagcctta
    24421 cctgcacggt tacgcgccac cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg
    24481 gaagccatca gcagtttctg atcgtcgctt tccatcagca tatggtcaac ggtcgcccca
    24541 ggcggcaacg ttaatttgcc ggtgagtggt tcgccctgac cacgcgccga cggcagcgta
    24601 atcgggtcga tggcatagct acgaccggtg gaatcaacaa acactaccgg ttggttgctc
    24661 ttacctttca ccgccgcttt gaagctatca cccgctttat aattcaggcc cggcgcgtcg
    24721 atatcatggc ctttagcgct gcgtacccag cccatctgcg acagcacaat ggtgacaggt
    24781 tcagacggca gcatgtcgtg ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc
    24841 gaacgacgat cgtcaccgta ggcttgcgcg tctgcctgca gttctttctt cagcaggtta
    24901 ttcattttac gctcggaagc caaaatgccc tgcaactggt cgcgctcttt ttccagttca
    24961 ctctgctcac cgcgaatctt catctcttcc agtttggcaa gatgacgcag tttcagttcg
    25021 aggatcgctt ccgcctgggt ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt
    25081 tcatcttcgt tacgaatgat ctcaatcact tcgtcgatgt tgagaaacgc caccagcaaa
    25141 ccttccagga tatgcaggcg cttgaggact ttctccagac gatagttcag tcggcggcgc
    25201 acggtatcgc gacggaacac cagccattcg gagaggattt ccagcaggtt tttcaccgcc
    25261 ggacgaccat ccagaccgat catattgagg ttaatgcgat agctcttttc cagatcggtg
    25321 gtagcgaaga ggtggttcat cacctgatcc atatccacgc ggttggaacg cggcacaatc
    25381 accagacggg tcgggttctc gtggtcagat tcatcgcgca gatcgtcaac catcggcagc
    25441 tttttgttgc gcatttgcgc agcaatttgc tccagtacgc gcgcacctga aacctgatgc
    25501 ggtaatgcgc tgataaccac cgcgccatct tctttcttcc acaccgcgcg catacgcact
    25561 gaaccacgtc cgttctcgta gattttacgg atctcggcgc gcgaagtgat aatttccgct
    25621 tcagtcggat aatccggccc ctgcacgata tccagcagct gatcgagcgt ggttttcggc
    25681 tggtcgatta atgcgattgc cgcctgagct acttcacgca ggttatgcgg tggaatatcg
    25741 gtcgccatgc cgacggcaat accggtggtg ccgttaagca aaatgtttgg cagacgagca
    25801 ggcagcattt tcggctcctg caaagtgccg tcgaagtttg gcacccagtc agccgtcccc
    25861 tgccccagct cgctcaatag cagctcggaa tatttcgaca accgggattc ggtgtaacgc
    25921 attgccgcga acgatttcgg atcgtccggc gcgccccagt tcccctgacc atcaaccagc
    25981 ggataacggt aagagaacgg ctgcgccatc aggaccatcg cttcataaca ggcgctatcg
    26041 ccgtgcggat ggtatttacc cagtacgtca ccgacggtac gggccgattt tttaaatttg
    26101 gcgctggcat tcaggcccag ttcagacatc gcatacacaa tgcggcgctg aacaggttta
    26161 agaccatcgc caataaacgg caacgcacgg tccatgatca cgtacatgga gtagtttaag
    26221 taggcgtttt ccgtaaattc atgtagcgca aggcgctctg ccatatcgct cattaattct
    26281 gattcctcaa cttattcgcc agccttcaaa ggggcaatat tgccggagat actacattat
    26341 ctgccgcgtc gagtcacagg agatcagcgt gaatagcgcc gccggacgtg ctccgacggc
    26401 gataaaggca tcaatgtgcc ttgatataca actctttgct gtagacttga tcggcataga
    26461 gggcagcaca aggttgcgcc catgccacac cactcggcat gggggtccgc tccgggcgat
    26521 tcgagaagtt gaggttatgc gagtccaggg aagcctaaag catcaaagta gtcgactgta
    26581 agtatacggt tcagcccagt ttagctgtgt ctccaccagt gccccgcgcc tcctgccata
    26641 agtgcggctt gctcatggat acccccgaga cggcttacgg cgcaccggat aataaatttc
    26701 gcgtatacat ggtattcctt accggagaaa ttataagccc aacaacaata cgcctgtggc
    26761 attgttcgct caaacttatc gcgagtgatt tggctcacac tccggtaaag attccccatc
    26821 acgccaacgg cacataaaca tccgtttcca gtcgctgctc tgtcacccct tcggcaagat
    26881 tggtgtagtg aaacagaatc ggtgctttac gcattttctc gccgcttgca ggcagccagt
    26941 ggcgaataat gccccatatc gtgtgtgaaa tatcgtccag ctcgccaacg tggcgggcca
    27001 cggcataacg tccaccggta agttcaccat tgctcacacc ataacgatta tcgggaatcg
    27061 gttcgctaac gctgccgcag atatcaaagc gaaacgcttc cggtgcggtg gtggcagggt
    27121 cgtcccaggc gacgccaaaa gtctggcttt ggttaacagg agaaagcccc gtctctttac
    27181 gccacatgat aaatttcgcc acactgtagt tgagcaactc agggctacag cggtgcggca
    27241 acatcgccac agggattgat gggaaatcga taatgtttac atccagtgtc aggtttgtca
    27301 ttttgcctcc ctgcgaaatt attcccggga acgcgtcccg ggagcggtaa caattacgga
    27361 tttactttgc ggatctgttt gacgtcaatt tcaacagaat tccagtcttt atcgacttca
    27421 ccctgaatct caaccgtatc tttcggcgtc accgtcacac cgttccagcg tttgtggtcg
    27481 atatcaacat tgatagtacc gctggcatct ttaaacacgt agagatcgtc agagatgcgt
    27541 tcaacgatat tgccgcgcag ggttacccag gtgtcgtcac gcagggattt tgcgctttct
    27601 acggtcgtta cgctgccgtt cggcccctgg aatcctccgg cctgactttg cgttgccgat
    27661 gggccagaaa aaccgccctg ctctgctgcc atcaccggtg cgctgcacag ggccattact
    27721 gcgattactg ctgcgaattt tttcatgttt attactccct ttaatgtctg tttccgagca
    27781 tttaacaaga tagtccttaa caacttctta agggaaaaaa ataaaattta gtgctgtaca
    27841 gagcgcgtta caacacggtt tactggcagc aaatacggtt atcgcaggga tgaaaaaatg
    27901 cgaattttac tgatagaaga tgacatgctg attggcgacg gcatcaaaac gggccttagt
    27961 aaaatgggtt ttagcgtcga ctggtttaca caaggtcgtc agggaaaaga ggcgctttat
    28021 agcgcgcctt atgatgcggt gatcctggat ttaaccttac caggcatgga tggtcgcgat
    28081 attttgcgcg aatggcgaga aaaaggtcag cgtgagccgg tactgatcct gaccgcgcgc
    28141 gatgcgctgg aggaacgtgt agaagggctg cgtctgggag ctgacgatta tctgtgtaaa
    28201 ccttttgcgt tgatagaagt cgccgccagg ctggaagctc tgatgcgccg aaccaacggc
    28261 caggccagca acgagctgcg ccacggtaac gtcatgctcg accccggcaa acgtatcgcc
    28321 acgctggctg gcgaaccctt aacactgaaa ccaaaagaat ttgccctgct ggaattactg
    28381 atgcgtaacg ctggtcgggt actgccgcgc aaactgattg aagagaaact gtatacctgg
    28441 gacgaagagg tcaccagtaa tgccgttgaa gtgcatgtgc atcatctgcg acgcaaactc
    28501 ggtagtgatt ttattcgtac cgtgcatggt attggctaca cattaggtga gaaatgaaat
    28561 ttacccaacg tcttagtctg cgcgtcaggc tgacgctaat ctttttaatt ctggcctcgg
    28621 tgacctggct gctttccagc tttgtcgcct ggaaacaaac aacggataac gtcgatgaat
    28681 tgttcgacac ccaactgatg ctgtttgcca agcggttaag tacgctcgat ctcaacgaaa
    28741 tcaacgcggc ggatcgcatg gcacagacgc caaataaatt aaaacacggt catgttgatg
    28801 acgatgcgct gacctttgcc atctttaccc acgacggcag aatggtcctt aacgatggcg
    28861 ataacggaga agatattcct tatagctatc aacgggaagg ttttgctgac gggcaactgg
    28921 tgggcgataa agatcaatgg cgttttgtct ggatgacctc acctgatggc aaatatcgca
    28981 tcgttgttgg tcaggaatgg gaataccgtg aagacatggc gctggcgatt gttgccgggc
    29041 aattgatccc gtggctggtc gcactgccga ttatgttaat catcatgatg gtactattgg
    29101 gtcgtgaact cgcgccgctg aacaaactgg cgctggcgct gcgtatgcgt gaccctgact
    29161 cggaaaaacc actaaacgcg actggcgtac ccagcgaagt gcgtccgttg gttgagtcgc
    29221 taaatcaact gttcgcccgc acacatgcga tgatggttcg tgaacgacgc tttacctccg
    29281 acgcagctca cgaactacgt agcccgttaa cggcgctgaa agtgcaaacc gaagttgcgc
    29341 agctttctga cgatgatccg caagcgcgga aaaaagcgct gcttcaatta cattacggga
    29401 tcgatcgcgc tactcgtctg gttgatcaac tgctcactct atcgcggctg gactcactgg
    29461 ataaccttca ggacgtcgcg gagatcccgc ttgaagatct cctccaatcg tcggtgatgg
    29521 atatttacca cacggcgcag caggcgaaaa ttgacgtgcg actgacactc aatgcccacg
    29581 gcatcaaacg caccggacaa ccgctattgc taagtttgtt ggtgcgaaat ttgctggata
    29641 acgccgtgcg ctacagtcca cagggcagcg tgatagacgt cacgctgaat gctgataatt
    29701 tcatcgtgag ggataacggc cccggtgtga cgccagaggc tctggcgcga attggcgaac
    29761 gcttctatcg cccccccgga caaaccgcta ccggcagtgg acttgggcta tcgattgtcc
    29821 agcgaatcgc caaattgcat ggcatgaatg ttgagtttgg gaatgcggaa caaggtggat
    29881 ttgaggcgaa ggtaagctgg taacttctct gtctatgaga gccgttaaaa cgactctcat
    29941 agattttact aatagcaaaa tataaaccgt ccccaaaaaa gccaccaacc acaaaccaga
    30001 caaataccga gcgagaatac atatacccaa aactcgaaca tttcccgcat aaagagtttt
    30061 cttaagataa gaataataag tggcgtaaga agaaaaaatg ctgcaatcat tgcttgcaat
    30121 atacgatctt cgcctgaaac ggaaggtata ctgcaaatat tgtctcccga ctcaagcatc
    30181 cagtcatact tattgaaagc aaaaaaagca tctattaacc aataagtaag cattatgtat
    30241 aacagagctt caaatat
//
LOCUS       DAZFXT010000053        29863 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.053, whole genome shotgun sequence.
ACCESSION   DAZFXT010000053 DAZFXT010000000
VERSION     DAZFXT010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.053"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            248..631
                     /gene="secE"
                     /locus_tag="ACHKW0_002514"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="ACHKW0_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HFO7475332.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="ACHKW0_002515"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="ACHKW0_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HFO7475333.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKW0_002516"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="ACHKW0_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HFO7475334.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKW0_002517"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="ACHKW0_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HFO7475335.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKW0_002518"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="ACHKW0_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HFO7475336.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKW0_002519"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="ACHKW0_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HFO7475337.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKW0_002520"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="ACHKW0_002520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HFO7475338.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKW0_002521"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="ACHKW0_002521"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HFO7475339.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12704..13837)
                     /gene="thiH"
                     /locus_tag="ACHKW0_002522"
     CDS             complement(12704..13837)
                     /gene="thiH"
                     /locus_tag="ACHKW0_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HFO7475340.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGHASQRP"
     gene            complement(13834..14604)
                     /gene="thiG"
                     /locus_tag="ACHKW0_002523"
     CDS             complement(13834..14604)
                     /gene="thiG"
                     /locus_tag="ACHKW0_002523"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HFO7475341.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14606..14806)
                     /gene="thiS"
                     /locus_tag="ACHKW0_002524"
     CDS             complement(14606..14806)
                     /gene="thiS"
                     /locus_tag="ACHKW0_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HFO7475342.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWAQHIVQDGDQILLFQVIAGG"
     gene            complement(14790..15545)
                     /gene="thiF"
                     /locus_tag="ACHKW0_002525"
     CDS             complement(14790..15545)
                     /gene="thiF"
                     /locus_tag="ACHKW0_002525"
                     /EC_number="2.7.7.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HFO7475343.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15538..16173)
                     /gene="thiE"
                     /locus_tag="ACHKW0_002526"
     CDS             complement(15538..16173)
                     /gene="thiE"
                     /locus_tag="ACHKW0_002526"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HFO7475344.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16173..18068)
                     /gene="thiC"
                     /locus_tag="ACHKW0_002527"
     CDS             complement(16173..18068)
                     /gene="thiC"
                     /locus_tag="ACHKW0_002527"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HFO7475345.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18149..18249)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18301..18777)
                     /gene="rsd"
                     /locus_tag="ACHKW0_002528"
     CDS             complement(18301..18777)
                     /gene="rsd"
                     /locus_tag="ACHKW0_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HFO7475346.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18872..19645
                     /gene="nudC"
                     /locus_tag="ACHKW0_002529"
     CDS             18872..19645
                     /gene="nudC"
                     /locus_tag="ACHKW0_002529"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HFO7475347.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19685..20749
                     /gene="hemE"
                     /locus_tag="ACHKW0_002530"
     CDS             19685..20749
                     /gene="hemE"
                     /locus_tag="ACHKW0_002530"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HFO7475348.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20759..21430
                     /gene="nfi"
                     /locus_tag="ACHKW0_002531"
     CDS             20759..21430
                     /gene="nfi"
                     /locus_tag="ACHKW0_002531"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="cleaves DNA at apurinic or apyrimidinic sites;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HFO7475349.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYILGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21473..22063
                     /gene="yjaG"
                     /locus_tag="ACHKW0_002532"
     CDS             21473..22063
                     /gene="yjaG"
                     /locus_tag="ACHKW0_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HFO7475350.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22250..22522
                     /gene="hupA"
                     /locus_tag="ACHKW0_002533"
     CDS             22250..22522
                     /gene="hupA"
                     /locus_tag="ACHKW0_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein HU-alpha"
                     /protein_id="HFO7475351.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22535..23230
                     /gene="yjaH"
                     /locus_tag="ACHKW0_002534"
     CDS             22535..23230
                     /gene="yjaH"
                     /locus_tag="ACHKW0_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HFO7475352.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23232..23657)
                     /gene="zraP"
                     /locus_tag="ACHKW0_002535"
     CDS             complement(23232..23657)
                     /gene="zraP"
                     /locus_tag="ACHKW0_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HFO7475353.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGTGMGMGYGGCGGGGHMGMGHW"
     gene            23895..25271
                     /gene="zraS"
                     /locus_tag="ACHKW0_002536"
     CDS             23895..25271
                     /gene="zraS"
                     /locus_tag="ACHKW0_002536"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HFO7475354.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25268..26593
                     /gene="zraR"
                     /locus_tag="ACHKW0_002537"
     CDS             25268..26593
                     /gene="zraR"
                     /locus_tag="ACHKW0_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HFO7475355.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26590..27879)
                     /gene="purD"
                     /locus_tag="ACHKW0_002538"
     CDS             complement(26590..27879)
                     /gene="purD"
                     /locus_tag="ACHKW0_002538"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HFO7475356.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(27891..29480)
                     /gene="purH"
                     /locus_tag="ACHKW0_002539"
     CDS             complement(27891..29480)
                     /gene="purH"
                     /locus_tag="ACHKW0_002539"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HFO7475357.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         6996 a         7783 c         8219 g         6865 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggt ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cgttgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacctggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggctgg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgcgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgc ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccag gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttcccgt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgta tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgatcg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat cctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaacatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg ggccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctaggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcacc gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacatcgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgtt ctgggtctgt actatatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgcaaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgcagcgcg
    10141 ttctggtgca tctgttggta tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgtgatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcgaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag ggtggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatatcctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc ctggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacaccc tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccacgcg ttgcggacct gttcgaagca cgtcgtccga aagagccggc
    11701 aatccttgct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgttaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgccgaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaattttt aagaaaggca gggcgaaacg aggaagaagc ttttcgccct ggacaacatt
    12601 cctgctgacg gaactaccat aaaaaaacag aacgctttca acaaccttgc gtttttcatc
    12661 ttccgtagcg gctcggaata aatttacgtt gttacattct gtctcatggc ctttgcgagg
    12721 cgtgtcccag atagctgtcc cagtctttcc ataccggctg caaaccctga gcggttaacg
    12781 cggccgcaac tgcttctggt ctgcgatcgt cgtgcggtga gaactgttcc agctcggggt
    12841 gattgtcggc atagccacct ggctgcgttt tcgaaaaagc gctgacgtta ttaattgcca
    12901 gcggaatcac gcgatcgcga aaccacggtg attcccgcgt ggagagtgag agttcaatct
    12961 ccggtgcaag cagtcggaag gcgcagatgg tttgcactaa ctggcgttca tccataatcg
    13021 acgcaggctc aatgccgcca gtacacgggc gcaggcgcgg aaaggagaca gagtaacggc
    13081 tttgccagta atgctgttgc agccatagca aatgttcggc aaccatatag cagtcaacgc
    13141 gccagttgtc ggaaaggcca attagcgcgc cgaggcctat cttatcaatc cccgcacgcc
    13201 ccagccgatc cggcgtttcc agccgccaga agaagtcctg ttttttgcct ttcaaatgat
    13261 ggcgggcata agtcgcctcg tgatatgtct cctgataaac catcacgcca tccagaccaa
    13321 gctgctttaa ctcggcgtat tccgtctccg ccagcggttg cacttccatt tgtagtgaag
    13381 agaactgttc acgcagggcg gggagatgac gacgaaagta atccatcccc actttcgcct
    13441 gatgttcacc agtgactaat agcagatgtt caaagcccat ctcccgtata gcggcgcttt
    13501 ccctggcaat atccgcttca tccagcgttt tgcgtttgat gcgattactc atggaaaatc
    13561 cgcagtacgt gcagtcgtta gcgcaaagat tggaaagata aagcgggacg tagaaactaa
    13621 ctgtgttgcc aaatcgctga cgggtcagac gctgcgcccg ttgggccagt tgttccagat
    13681 agccactggc ggctggcgat aacagcgcca tcatatcgtc gcgggtgagt tgcgaggcat
    13741 ttagcgcccg ttctacgtca gcagccgttt tgccgttgat acgcaggcgg atgtcgtccc
    13801 agtccagttg tcgccagcga tcgctgaagg ttttcatgcc gatgcctcca gaaatccagt
    13861 cagcgggctg gtggcatgag caaaatgact gcggctgccc ggtccggact gacgtgccag
    13921 caggcctgct tctaccgcca gacgaaatgc cttcgccatg ttgacgggat cgtccgcgac
    13981 ggcaattgcc gtattcacta acaccgcgtc ggcccccatt tccagcgcct gcgcggcatg
    14041 gctgggaacg ccgatgccag catcgacaac caccggcact gtggcctgct ggataataat
    14101 ctccagcatg gcgcgggttt ccagtccctg attcgagcca atcggcgcgc cgagcggcat
    14161 caccgctgca cagccgactt cttccagacg tttacacaat accggatcgg ccccgcagta
    14221 aggcagcacg acaaatccct gttgtaccag cgtttcggcg gctttcaggg tttcgatggg
    14281 atcgggcaac agccagcggg cgtcagggtg aatctctaat tttaaccagt ttgtgcctaa
    14341 cgcttcacga gccagatggg cggcgaaaat ggcttcttcc gctgttttcg ccccggatgt
    14401 atttggcagc agggtcacac ccgccgcgat aagcggttcg aggatagcgt cgttgtgctg
    14461 gcgcaagtcg acacgtttca tcgccagtgt caccagctgg ctgccggaag cgcggatcgc
    14521 ctccaccatc agttgtgaag aagcgaattt ccctgtgccg gtaaacagat gtgaatcaaa
    14581 cgttttgtcc gcaatacgta acatttcaac cccctgcaat aacctgaaaa agcaggatct
    14641 ggtcgccatc ctgcacgata tgttgcgccc actgctcacg cgggacgatt tgctgattaa
    14701 tcgccagagc cgcgcccgct tgtcgttggt cgagttgctc cagtagttcg tgaacagttt
    14761 gcccggcggc gcactgcatc ggttgatcgt taaacaggat ctgcattgct tcctccgcat
    14821 accgggcaac cactggcgcg gcgcaacgcc aggctgcgcc actggctcga tttaccgtcg
    14881 aacagtcgga gttctcccgc aggtgtctct ataccgctta ataacttaat ggcttccagt
    14941 gcctgcaaag tgcccataac cccgaccacc gggccaacca cgcccgccgt gcggcagttg
    15001 cgttctggct cctggttatc tggccacagg cagcggtaac acccctgctc ccagggcggc
    15061 gtcagtacca tcaactgacc gccaaatccg accgcgctgg cggtgataag cggcgtgttg
    15121 agtgccacgc aggcggcatt aatctcctgg cgagtcgcca tattgtcggt acagtcgagc
    15181 accacatcgg cccgtgcaac cgcatctttt aacgcctcac ccgttaaccg ttgttgtaat
    15241 gctgtcagtt gaatgtcggg attcaactgt gtcagtcgct gttggctgac ctgcgatttc
    15301 gggctgttga tatcttcagt ggtaaagagg atttgtcgtt gcagattgct taaatgcaca
    15361 tcgtcgtcat ctgccagtac cagcgtcccg acgccagcgc ccgccaggta cagcgcagca
    15421 ggtgtaccca gcccgcccag accgataatc agcacctggc tgtcgagcag tttttgctgc
    15481 ccgtccagag cgatatcgtc gagcaggatt tggcggctat aacgcataaa gtcacggtca
    15541 ttcatcgcca actcctgcaa tttccagcaa ctgtgccgtt gccaaacgcc agtctgcagc
    15601 ttgagtaatg gcgctgacga cggcgatact gccgacaccc gttgctatca ccgcaggcgc
    15661 gcgtgccaga ctgataccgc caatcgccac ggtgggataa tccgccagtc gctcaacatg
    15721 ccgtgccagc tgttccagcc cctgcggtgc agaaggcatc tgtttggttt gcgtcgggaa
    15781 cacatgtccc agcgcgatat aagaggggcg tgctgccagc gcgacgtcga tttccatatc
    15841 gtcatgtgtc gaaacgccca gccgcaggcc tgccgcgcgg atggcattga gatcggtggc
    15901 ttgcaaatct tcctgcccca aatggacgcc atacgcctga tgcttgatcg ccagccgcca
    15961 gtaatcgttg ataaacaatc gcgcgttata gcggcggccc agcgcaattg ccgccacgac
    16021 atcggcttcc acctcttcat cgcgccgatc tttgatgcgt agctggagag tacgtacgcc
    16081 tgcatccaac agacgttcga tccactgtac gctgtccacc accgggtaca gtcctaaacg
    16141 aaaaggtaca ggaggaaaat caggctgata catcacgctt cctccttacg caggtagatt
    16201 tctccgcctc tggcacggaa gttctccgac atatccgcca ttcccatttc aatagtttgc
    16261 gtggcggcgt aatcacgcac ttcctggctg attttcatcg aacagaattt cggcccacac
    16321 atggagcaaa aatgggcgac tttacctgac tcttgcggca gggtttcatc gtgataagcg
    16381 cgggcggtaa acgggtcgag ggccagatta aactggtctt cccagcgaaa ttcgaagcgg
    16441 gctttcgaca tggcgttatc gcgaatttgc gcgcccggat gccctttcgc caggtcagcg
    16501 gcgtgggcgg caatcttata ggtgataagc ccctgcttaa catcttcttt attaggcaga
    16561 cccagatgct cttttggcgt tacgtaacag agcatcgcgc agccaaacca gccaatcatc
    16621 gccgcaccaa tccccgacgt gaagtggtca tagcccggcg caatatcggt agttagcggc
    16681 cccagagtgt aaaacggcgc ttcgtggcag tgctctaact cctcggtcat attgcggcgg
    16741 atcatctgca tcggcacgtg acctgggcct tcaatcatca cctgcacgtc atattcccag
    16801 gcaattttgg tcagttcgcc cagcgtatgc agctcggcaa actgcgcttc atcgttggcg
    16861 tcctgaatag aaccggggcg cagaccgtcg cccagcgaca gcgaaacgtc ataagcggca
    16921 cagatttcac aaatttcgcg gaagtgttga tagaggaaat tttcctgatg atgggagagg
    16981 caccatttcg ccataatcga accgccgcga gagacgatac cggtcaggcg tttcgcggtc
    17041 atcggcacat agcgcagcag tacgcccgca tggatagtga agtaatccac accttgctcg
    17101 gcctgttcca gcagcgtgtc gcggaacgct tcccaggtaa gatcttcggc gatcccgtta
    17161 accttctcca gcgcctggta gatcggcact gtaccgatcg gcaccgggct gttacgcaaa
    17221 atccactcgc gggtttcgtg aatatagcga ccggtggaga gatccatcac cgtatccgct
    17281 ccccagcgcg tggaccatac cagcttttcc acttcttctt cgatggaaga ggtgaccgcc
    17341 gagttgccga tattggcgtt aacttttacc aggaaattgc gaccaataat catcggctcc
    17401 gattccggat gattaatgtt ggccgggata atcgcacgtc cggcagcaac ttcatcacgg
    17461 acaaattccg cagtgatatt ttccggcaga tgtgcgccaa agctcattcc cggatgctgg
    17521 tggcgtaaaa cctcgctacg gatgcgctcg cggcccatat tctcgcggat ggcgatgaat
    17581 tccatttccg gcgtgatgat gccctggcgg gcgtagtgca gttgggtgac acggcgtcct
    17641 gctttggcgc gttttggtgt tagtacaccg ctaaaacgca gttcgtcgag gccatcatct
    17701 gccagccgcg ctttagtgta atcggaactg cgcacggtaa gttcttcggt atcgccgcgc
    17761 gcatcgatcc acggctggcg tagttttgcc agcccttgct gcacgttaat ggcaatctgc
    17821 ggatcaccat acgggccgga ggtgtcgtag accggaatcg cttcgttttc ttcgtactgc
    17881 ggctgttctt tgctaccgcc aattagcgtc gggctaagct ggatctcacg catcggcacg
    17941 cgcacgccgg ggtgtgtgcc agtgatataa atgcgttttg agttgggaaa ggcggtgcct
    18001 tccagggtgt cgataaaatg ttgggcccgg gcgcgttgtt cgcggcgggt cagttttgtt
    18061 gcagacatag ctcattccaa aaagttaagg acgtggcttg tcagacgacg gatgaagcaa
    18121 gagacgatcg ccgcaggggc gatgcgatag cagattaact cttgttccct tcgcaggtat
    18181 tagcctgatc aggttccgcg gatcccgaat aaacggtctc agccagttaa ggcactccga
    18241 caagaaatta gccccgcaaa tggggcattg aatgtaaatt acgcgttaac agcgcagaac
    18301 tcaagcagga tgtttgacgc gggcggcgtc aagcaccagc agaatcagct tatcttccag
    18361 cacaaagcgc gcttccagcg cctcgccgat gtcagataaa acctgttgaa actcaaggta
    18421 attatcatga tcgatggcgg tttccagact ggaatcgtag taatccataa tctgttgagt
    18481 attggcttcg agttgcggcc aaatcttcgc ggcgcgcgcg agttgcccgt tgccttccag
    18541 cttatgaaga atgcgctcat aaatactgaa atgtccggca gacaagtaat cgaccaggct
    18601 ctgacaaaaa tcatcaaggg ctttttcgtt tagcctcatg tacgattctt tgccaggctt
    18661 aatgccaacc agattgtagt aagccacgag cagatgctta cgtacatgta gccagcgatc
    18721 aaccagtttg ttacttcctc tgacgcgctc cgtcaggtta tcgagctggt taagcatgat
    18781 tgactccgca agtttgtatt caaaaactgc tcagtgagaa atgtaaaaac catgttaaac
    18841 atgccagtga tgcaaaggta gtgcaagagc tatggatcgt ataattgaaa aattagatca
    18901 cggctggtgg gtcgtcagcc atgaacaaaa attatggttg ccgaagggag aattgccata
    18961 tggcgaagcg gcaaatttcg atcttgtggg tcagcgcgca ctacaaatcg gcgaatggca
    19021 gggggaacct gtttggttag tacaacagca gcggcgtcac gatatggggt cggtacgtca
    19081 ggtcattgat ctcgatgttg ggctgtttca actggccgga cgaggcgtac aactggcgga
    19141 gttttaccga tcgcataaat actgtggtta ctgcgggcat gaaatgtatc cgagcaaaac
    19201 cgaatgggcg atgctgtgca gccattgccg tgagcgttac tacccgcaaa tcgccccctg
    19261 cattattgtt gccatccgtc gcgatgattc gatcctcctc gcccagcata cccgccatcg
    19321 taacggtgtc catacagtac ttgccggatt cgtcgaagtg ggcgaaaccc tcgagcaggc
    19381 agtcgcgcgg gaagtgatgg aagagagcgg aattaaagtt aaaaacttgc gttacgtgac
    19441 ttctcagccg tggccgtttc ctcagtcttt aatgaccgcg tttatggcgg aatatgacag
    19501 cggcgacatc gtgatcgacc cgaaagaatt gctcgaggcg aactggtatc gctatgacga
    19561 tttgccgtta ctcccgccgc ccggcaccgt agcgcgccgt ctgatagaag atacggtggc
    19621 gatgtgtcgg gcagagtatg agtgatgata cactgaccgc ctgacgcact aaggaacagc
    19681 caaaatgacc gaacttaaaa acgatcgtta tctgcgggcg ctgctgcgcc agcccgttga
    19741 tgtcactcca gtatggatga tgcgccaggc gggtcgctat ctaccggaat ataaagccac
    19801 gcgcgcccag gcgggcgatt ttatgtcgct gtgcaaaaac gccgagctgg cgtgcgaagt
    19861 gactttgcaa ccgctgcgtc gctacccgct ggatgcggcg atcctctttt ccgatatcct
    19921 caccgtgccg gacgcgatgg ggttagggct ctattttgaa gccggagaag gtccgcgttt
    19981 tacctcgcca gtcacctgca aagccgacgt cgataaactg ccaattccgg acccggaaga
    20041 tgagctgggt tacgtgatga acgcggtgcg taccattcgt cgcgaactga aaggcgaagt
    20101 gccgctgatt ggtttttccg gcagcccgtg gacgctggca acctacatgg tggaaggcgg
    20161 cagcagcaaa gccttcaccg tgatcaaaaa aatgatgtat gccgatccgc aggcgctgca
    20221 cgctctactc gataaactgg cgaaaagcgt cactttgtat ctgaatgcgc agattaaagc
    20281 cggtgctcag gctgtgatga ttttcgacac ctggggcggc gtgcttaccg ggcgcgatta
    20341 tcaacagttc tcgctttatt acatgcataa aattgttgat ggtttactgc gtgaaaacga
    20401 cggtcgccgc gtaccggtca cgctgtttac caaaggcggc ggacagtggc tggaagccat
    20461 ggcagaaacc ggttgcgatg cgctgggcct cgactggaca acggacatcg ccgatgcgcg
    20521 ccgccgtgtg ggcaataaag tcgcgttgca gggtaatatg gatccgtcga tgctgtacgc
    20581 gccgcctgcc cgcattgaag aagaagtagc gactatactt gcaggtttcg gtcacggcga
    20641 aggtcatgtc tttaaccttg gtcacggcat tcatcaggat gtgccgccag aacatgctgg
    20701 cgtgttcgtg gaggcagtgc atcgactgtc tgaacagtat caccgctaag gagtgattat
    20761 ggatctcgcg tcattacgcg ctcaacaaat cgaactggct tcttctgtga tccgcgagga
    20821 tcgactcgat aaagatccac cggatctgat cgccggagcc gatgtcgggt ttgagcaggg
    20881 cggagaagtg acgcgagcgg cgatggtgct gctgaaatat ccctcgcttg agctggtgga
    20941 gtataaagtt gcccgcatcg ccaccaccat gccttacatt ctaggttttc tttccttccg
    21001 cgaatatcct gcgctgctgg cagcgtggga gatgctgtcg caaaagccgg atttagtgtt
    21061 tgtcgatggt catgggatct cgcatcctcg ccgtcttggc gtcgccagcc attttggctt
    21121 attggtggat gtgccgacca ttggcgtggc gaaaaaacgg ctctgcggta aattcgaacc
    21181 gctctccagc gaaccgggcg cgctggcccc actgatggat aaaggcgagc agctggcctg
    21241 ggtctggcgc agcaaagcgc gctgtaaccc gttgtttatc gctaccggcc atcgggtcag
    21301 cgtggacagc gcgctggcgt gggtacaacg ctgcatgaaa ggctatcgtc tgccggagcc
    21361 aacgcgctgg gcggacgcgg tggcctcgga acgtccggcg ttcgtgcgct atacagcaaa
    21421 tcagccctaa ttcaggtaaa ctgcgggtaa tttccgtatt tgagaactca acatgttaca
    21481 aaacccaatt catttgcgtc tggagcgcct ggaaagctgg cagcacgtca ctttcatggc
    21541 ttgcttatgc gaacgcatgt accccaatta cgccatgttt tgccagcaaa ccggttttgg
    21601 tgatgggcaa atttaccgtc gtattctcga tctcatctgg gaaacgctga ccgttaaaga
    21661 cgcaaaagta aatttcgaca gccaactgga gaaatttgaa gaagcgattc cttcagccga
    21721 cgatttcgat ctgtacggcg tttatccggc aatcgatgcc tgcgtggcgt taagtgaact
    21781 ggtccattcg cgtttgagtg gtgaaacgct cgaacacgcg gtggaagtga gtaagacctc
    21841 catcacgacc gttgcgatgc tggaaatgac tcaggctggt cgtgaaatga gcgatgaaga
    21901 gcttaaagaa aacccagctg tagagcaaga atgggacatt cagtgggaaa tattccgact
    21961 tttagctgag tgcgaagaac gcgatatcga gctgataaaa ggccttaggg cagacctgcg
    22021 tgaggcgggt gagagcaata ttggtataat ttttcagcaa taagaccaga aaacgtgatt
    22081 taacgcctga tttgtcgtac ctggagtctt ccctttcgcc ccccgtctgg tctacatttg
    22141 gggggcgaaa aaaagtggct atcggtgcgt gtatgcagga gagtgctatt ctggcatttc
    22201 cgtcgcactc gatgcttagc aagcgataaa cacattgtaa ggataactta tgaacaagac
    22261 tcaactgatt gatgtaattg cagagaaagc agaactgtcc aaaacccagg ctaaagctgc
    22321 tctggagtcc actctggctg caattactga gtctctgaaa gaaggcgatg ctgtacaact
    22381 ggttggtttc ggtaccttca aagtgaacca ccgcgctgag cgtactggcc gcaacccgca
    22441 gaccggtaaa gaaatcaaaa ttgccgcagc taacgtaccg gcatttgttt ctggcaaggc
    22501 actgaaagac gcagttaagt aagattgcgt ggcagtgaac agttttaacg aaggggtggt
    22561 ttcacccctt ttgtctttct ggcgtcgatc attgatgctg gcaggcgctc tgtttctcac
    22621 tgcctgtagt cacaactctt cacttcctcc ctttaccgcc agtggatttg ctgaagacca
    22681 gggcgcggta cgcatctggc gaaaagacag cggcgataat gtgcatctgc ttgccgtgtt
    22741 tagcccgtgg cgcagtggcg ataccacgac gcgagagtat cgctggcagg gcgataacct
    22801 cacgctcatc aatatcaatg tttacagcaa accgccggtg aacattcgcg cgcgttttga
    22861 cgatcgcggc gatctgagct ttatgcaacg tgaatccgat ggggaaaagc agcagctttc
    22921 taacgaccaa atcgatttat accgttatcg tgctgatcag atccgccaga ttagcgatgc
    22981 cttacgtcag gggagagtcg tgctgcgcca ggggcgctgg catgcgatgg aacagaccgt
    23041 gaccacctgc gaagggcaaa ccattaaacc tgatttagat tcgcaggcga tagcgcatat
    23101 cgagcgccgc cagagccgct cttctgttga tgtcagcgtg gcatggctgg aagcgcccga
    23161 aggttcgcaa ttactgttag tggcaaactc tgatttctgt cgctggcaac ccaacgagaa
    23221 aacgttctga tttaccagtg gcccataccc atatgaccgc caccaccgca gccgccgtaa
    23281 cccatgccca ttccggtacc gcgcggaata cccgcttcag ccatcgcaat atctcgtttc
    23341 acccgtaact catctaacga ctgacgcaaa ttctccatct ctttggcgac cgcgttaatt
    23401 ttgctgctat ccggtgggtt cgcggctaac agggcattgt attcataacg cttcgtcacc
    23461 agttgctgtt gcagtgcgct gctttgagcg taaaagtcat tatggatttt ctgccacgct
    23521 gtctgttgtt cgctggtcaa aggcgcggca ttttgctgcc acataccgtg tccgccgtga
    23581 gcaaatgcag atgtcgatcc catcgccatt gctgaaagcg ccatcattac cagggcaatt
    23641 ttcgtgttcc gtttcatggt taatcctcca gtggttgtct tacttcgggt attgcatctt
    23701 ccgtgccaac gatgaaacgc tgatatgacg ggtaatctgg catgataaac gagtaaaaat
    23761 gactcgcctg ctgcgggtag cgagtcattt ttactcattg aaacttaagc ctttgtgtta
    23821 cagcgcaggg taagcgctga taaaagatgg catgatttct gctgtcagaa agggatgagc
    23881 aggcaaagaa gaagatgcgt tttatgcaac gttctaaaga ctccttagct aaatggttaa
    23941 gcgcgatcct ccccgtggtc attgttgggc tggtagggtt gtttgcggtg acggtgattc
    24001 gcgattatgg gcgcgagact gccgccgcca gacaaacgct gctggaaaaa ggcagtgtac
    24061 ttattcgcgc tcttgaatcc ggctcgcgcg tcggcatggg gatgcgcatg catcatgcgc
    24121 agcagcaggc cttactggaa gaaatggccg ggcagcctgg agtacgttgg tttgcggtca
    24181 cggatgaaca aggaacaatc gtgatgcata gcaactccgg catggtggga aaacagcttt
    24241 attccccgca ggaaatgcag cagttacatc ctggagatga agaagcgtgg cggcggatcg
    24301 atagcgcaga cggcgagcct gttctggaaa tttatcgcca gtttcaaccg atgtttgctg
    24361 ctggaatgca ccggatgcgc catatgcagc agtatgccgc aacaccacaa gcaattttca
    24421 tcgctttcga cgccagtaat attgtgagtg ccgaagatcg tgagcagaga aacaccctga
    24481 ttatcctctt cgccctggcg acggtcttgc tggcaagcgt attgtcattc ttctggtatc
    24541 gccgctatct gcgctcgcgc cagcttctac aagatgaaat gaagcgcaaa gagaagctgg
    24601 tggcgctggg gcatcttgcg gcaggcgttg cccacgaaat ccgtaaccca ctttcctcga
    24661 ttaaaggact ggcgaaatac tttgccgaac gcgcgccagc agggggagaa gcgcatcaat
    24721 tggcgcaggt gatggcgaaa gaagccgacc gtttaaaccg cgtggtaagc gagttgctgg
    24781 aactggttaa gccaacgcat ctggctttgc aggcggtgga tctcaacacg ctgattaacc
    24841 actcattaca gctggtaagc caggatgcaa acagccggga gatccagtta cgctttaccg
    24901 ccaacgacac attaccggaa attcaggccg atccggacag gctgactcag gtcctgttga
    24961 atctctatct caatgctatt caggcgattg gtcagcatgg cgtgattagc gtgacggtca
    25021 gcgaaagcgg cgcgggcgtg aaaatcagcg ttaccgacag cggtaaggga attgcggcag
    25081 atcagcttga agccatcttc actccgtact tcaccaccaa agccgaaggc accggattgg
    25141 ggctggcggt cgtgcataat attgttgaac aacacggtgg tacaattcag gtcgcaagcc
    25201 aggagggaaa aggctcaacg ttcaccctct ggcttccggt caatattacg cgtaaggacc
    25261 cacaaggatg acgcacgata atatcgatat tctggtggtg gatgatgaca ttagccactg
    25321 cactattttg caggctttac tgcgcggctg gggctataac gtcgcgctgg cgaacagcgg
    25381 gcgacaggcg ctggagcagg tgcgggaaca ggtttttgat cttgtgcttt gcgatgtgcg
    25441 aatggcggag atggacggca tcgccacgct gaaagagatc aaagcgttaa acccggcaat
    25501 tccggtgctg attatgactg cgtactccag cgtcgagacg gcggtagagg cactgaaaac
    25561 tggggcgctg gattatctca tcaagccgct ggatttcgat aacctacagg cgacgctgga
    25621 aaaggcgctc gcgcatacgc acagtattga tgctgaaaca cctgcggtga ctgccagcca
    25681 gttcggtatg gtcggtaaaa gctcggcgat gcaacacctg ctcagtgaaa tcgccctcgt
    25741 cgcgccatcg gaagccacgg tactgatcca cggcgattcc ggcaccggta aagagctggt
    25801 cgccagggcg attcacgcca gtagcgcacg tagcgaaaaa ccgctggtaa cgctcaactg
    25861 tgcggcactc aacgaatcct tgctggaatc tgaattgttc ggtcacgaaa aaggggcgtt
    25921 tactggagcc gataaacgcc gggaagggcg ctttgttgag gcggacggcg gcacgctgtt
    25981 tctcgatgaa attggcgata tctcgccgat gatgcaggtg cgtctgctgc gtgcgattca
    26041 ggagcgcgaa gttcagcgtg tcggtagcaa ccagactatc tcggttgatg tccggctgat
    26101 tgcggcgacc catcgcgatc ttgccgcaga ggtgaatgcc gggcgttttc gccaggatct
    26161 ctactatcgc ctgaatgtgg tggcgattga agtaccatcg ctgcggcaac ggcgggaaga
    26221 tatccctctg ctggctggcc attttctgca gcgctttgcc gagcgtaatc gcaaggcggt
    26281 aaaaggtttt acgccccagg cgatggatct gttgattcat tacgactggc cgggaaatat
    26341 tcgtgagctg gaaaacgcgg tggaacgggc agtggtgctg ctgaccgggg aatatatttc
    26401 cgaacgcgag ctgccgctgg cgattgccag tacgccgatc ccgctggggc aaagtcagga
    26461 tattcagccg ctggtggaag tggaaaaaga ggtgattctg gcggcactgg agaaaacggg
    26521 cggcaacaaa actgaagccg cccgtcagtt agggatcacg cgtaaaacgt tattggcaaa
    26581 actgtcgcgt tagttctgct cgcgttcgat agcacgccag ccgatatctt tccggcagaa
    26641 gcagtcgtcc cagtggatat cggtcattaa ggcataggcg cgtttctgcg cttctgccac
    26701 ggtatgaccc agcgcggtga cgcacagtac gcgtccgccg ttggttacca cctgctcgtc
    26761 atccgccagt tttgtgcccg cgtggaacac tttgccgtca gccacttctt ccagcggcag
    26821 gccgtggatc acatcgccgg tgcggtaatc gcccggatat ccacccgcag ccatcaccac
    26881 gccgagagaa gcgcgttcat cccactcgga cgttttctcg tccagcttgc cttcacaggc
    26941 cgccaggcag agctcaacca gatcggactt catgcgcagc ataatcggct gggtttccgg
    27001 atcgccaaag cggcagttaa attcaataac cttcggattg ccctgtttgt cgatcatcag
    27061 gcccgcatag agaaaaccgg tgtaggtgtt gccttccgcc gccatgcctt tcacggttgg
    27121 ccagataata cgttccatgg tgcgctgatg aacgtcatcg gttactaccg gcgcggggga
    27181 gtaagcgccc atcccgccgg tgtttggtcc ggtatcttta tcgcctacgc gtttgtgatc
    27241 ctggctggta gccatcggca gcacatgctc gccgtccacc atcacgataa agctcgcttc
    27301 ttcgccatcg aggaactctt cgataacgat gcgatgaccc gcgtcgccaa aagcgttgcc
    27361 cgccagcata tcgtgaacag ccgcttccgc ttcttccagc gtcatcgcca cgataacgcc
    27421 tttcccggca gccagaccgt ccgctttaat gacgattggc gcgcctttct cacgcagata
    27481 cgccagcgca ggttctacct cggtgaagtt ctggtattcc gccgtaggga tcttatggcg
    27541 ggccaggaaa tctttggtaa acgcttttga gccttccagt tgggccgcac ctgcggttgg
    27601 gccgaagatt ttcagcccgg cggcgcggaa ggtatcgacc acgcctttca ccagcggcgc
    27661 ttccgggccg acgatggtca gatcaatctt ttcgttttgt gcgaaatcca acagcgccgg
    27721 gatatcggtc acgccaatag caacgttttg cagcgcgggt tccagcgcag tgcctgcatt
    27781 acccggagca acaaaaacag tctcaaccag cggcgactgg gccgctttcc aggccagcgc
    27841 gtgctcgcgc ccgccgttac caatcactaa tactttcatc tattgctcca ttaatggcgg
    27901 aagtggcgca tgtcggtgaa gagcatcgca ataccgtgct cgtcggcggc ggcaatcact
    27961 tcgtcatcac ggatggaacc gccaggctgg attacgcagg tcacgcccgc agcggcggcg
    28021 gcatcaatac cgtcgcggaa cgggaagaac gcgtcagaag ccatcgagga acctttcact
    28081 tccaggcctt catcagcagc tttaataccg gcgattttcg cggagtacac gcggctcatc
    28141 tggcccgcgc caataccgat ggtcatattg tttttggcat agacgatagc attggatttc
    28201 acgaacttcg ccactttcca gcagaacagc gcatcgcgca gttcctgttc ggtcggctga
    28261 cgttgggtga cgacgcgcag ttcttccgca ccgaccatcc ccaggtcacg atcctgaacc
    28321 agcagaccgc cgttcacgcg tttgaaatca agacccggaa cacgctcgcc ccactgaccg
    28381 caggtcagaa cgcgtacgtt ctgcttggcg gcggtgattt tcagggcttc ttcgctggcg
    28441 gaaggcgcaa taatcacttc gacaaactga cgagaaatga tggcctgtgc ggtttccgca
    28501 tccagctcgc ggttaaaggc aatgatgccg ccgaatgcgg aggttgggtc ggttttgtac
    28561 gcgcgatcgt aagcatcaag aatggaattg ccgatagcta caccgcaagg gttggcgtgc
    28621 ttcacaatca cacatgccgg ctcggcgaac tctttcacgc actccagcgc cgcatcggta
    28681 tcggcgatgt tgttataaga gagggcttta ccctgaacct gggttgcggt agcaacggag
    28741 gcttctttca cattctcttc tatatagaag gcagcctgct ggtggctgtt ctcgccgtaa
    28801 cgcatatcct gcttcttaat gaagttcagg ttcagcgtgc gtgggaagcg accagcggct
    28861 tctttgcttt caccgtggta cgccggaacc atgctgccga agtagttggc aatcatgctg
    28921 tcgtaggcgg cagtgtgttc gaaggctttg atggcgaggt cgaaacgggt tgcaagcgtc
    28981 agcgatcctt cgttgtcatc catctcttta ataatggcgt cgtagtcgct gctcttcacc
    29041 acgattgcga catctttatg gttcttagcg gcggagcgca ccatggtcgg gccgccgata
    29101 tcgatgttct caaccgcatc ttccagcgag caaccttcac gggccacggt ctgggcgaac
    29161 ggatacaggt taacaaccac catatcgata ggctggatct gatgttcttc cataatggca
    29221 tcgtcctggc cgcgacggcc cagaatgcca ccatgtactt tcggatgcag ggtcttcacg
    29281 cgtccatcca tcatctccgg gaaaccggtg taatcggaaa cttcggttac cggcagacct
    29341 ttttctgcta acagacgggc agtgccccct gtagacagca gctccacacc gcgtgcggaa
    29401 agtgcctggg cgaattcgac gataccggct ttgtcagaaa cactgagcag agcgcggcgg
    29461 actggacgac gttgttgcat ggtaaatccc ctggatttga ctattacaga gagcgttagc
    29521 tgaatttttc gcgaaaaact cagctaacgc ccctaacggg gcatccttat ttttcgcccg
    29581 cattgtaacg aaaacgtttg cgcaacgctc gcgaattttt ctctttcaat ggtgatcaca
    29641 attttgacag tggttaccgt gggaaaaata cagaaattac attgatgatt gtggataact
    29701 ctgtgcgtaa aaaggtataa agcggggttt tgctggggaa tgcagcagtc agtcattttt
    29761 ctgcaatttt tctattgcgg cctgcggaga actccctata atgcgcctcc atcgacacgg
    29821 cggatgtgaa tcacttcaca caaacagccg gttcggttga aga
//
LOCUS       DAZFXT010000054        29651 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.054, whole genome shotgun sequence.
ACCESSION   DAZFXT010000054 DAZFXT010000000
VERSION     DAZFXT010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.054"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKW0_002540"
     CDS             complement(130..777)
                     /gene="gloC"
                     /locus_tag="ACHKW0_002540"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HFO7475358.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(804..1352)
                     /locus_tag="ACHKW0_002541"
     CDS             complement(804..1352)
                     /locus_tag="ACHKW0_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF882 domain-containing protein"
                     /protein_id="HFO7475359.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHKW0_002542"
     CDS             complement(1533..3380)
                     /gene="ldtD"
                     /locus_tag="ACHKW0_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7475360.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHKW0_002543"
     CDS             complement(3641..8101)
                     /gene="mukB"
                     /locus_tag="ACHKW0_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HFO7475361.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVLPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRAPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHKW0_002544"
     CDS             complement(8101..8805)
                     /gene="mukE"
                     /locus_tag="ACHKW0_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HFO7475362.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDENEENQPDSGEEE"
     gene            complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHKW0_002545"
     CDS             complement(8786..10108)
                     /gene="mukF"
                     /locus_tag="ACHKW0_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HFO7475363.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPPDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHKW0_002546"
     CDS             complement(10105..10890)
                     /gene="cmoM"
                     /locus_tag="ACHKW0_002546"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HFO7475364.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            11026..11805
                     /gene="elyC"
                     /locus_tag="ACHKW0_002547"
     CDS             11026..11805
                     /gene="elyC"
                     /locus_tag="ACHKW0_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HFO7475365.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(11782..12675)
                     /locus_tag="ACHKW0_002548"
     CDS             complement(11782..12675)
                     /locus_tag="ACHKW0_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HFO7475366.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYLQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHKW0_002549"
     CDS             complement(12829..13575)
                     /gene="kdsB"
                     /locus_tag="ACHKW0_002549"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HFO7475367.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAAPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAKDLETVGDNFLRHLGIYGYRAGFIRRYVTWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHKW0_002550"
     CDS             complement(13572..13754)
                     /gene="ycaR"
                     /locus_tag="ACHKW0_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HFO7475368.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHKW0_002551"
                     /pseudo
     CDS             complement(13806..15038)
                     /gene="ycaQ"
                     /locus_tag="ACHKW0_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ"
     gene            complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHKW0_002552"
     CDS             complement(15075..16061)
                     /gene="lpxK"
                     /locus_tag="ACHKW0_002552"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HFO7475369.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYSGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHKW0_002553"
     CDS             complement(16058..17806)
                     /gene="msbA"
                     /locus_tag="ACHKW0_002553"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0034040 - ATPase-coupled lipid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HFO7475370.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFDHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(17843..20107)
                     /locus_tag="ACHKW0_002554"
     CDS             complement(17843..20107)
                     /locus_tag="ACHKW0_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HFO7475371.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTIFACVLAFIPV
                     KTVRYIALTLLFFVWGILAAKQILWAGETLTGATQDAIVEITTTDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAIHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYMWNAVILGLGMGVRLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGRICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     FAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAGGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGMNSILLTGDIEVPAEQKMLSRYWQQVQATLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYTWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHKW0_002555"
     CDS             complement(20314..20598)
                     /gene="ihfB"
                     /locus_tag="ACHKW0_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA];
                     GO_process: GO:0075713 - establishment of integrated
                     proviral latency [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HFO7475372.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHKW0_002556"
     CDS             complement(20758..22431)
                     /gene="rpsA"
                     /locus_tag="ACHKW0_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HFO7475373.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHKW0_002557"
     CDS             complement(22542..23225)
                     /gene="cmk"
                     /locus_tag="ACHKW0_002557"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - (d)CMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HFO7475374.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHKW0_002558"
     CDS             complement(23398..24162)
                     /gene="ycaL"
                     /locus_tag="ACHKW0_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HFO7475375.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHKW0_002559"
     CDS             complement(24331..25614)
                     /gene="aroA"
                     /locus_tag="ACHKW0_002559"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HFO7475376.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHKW0_002560"
     CDS             complement(25685..26773)
                     /gene="serC"
                     /locus_tag="ACHKW0_002560"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HFO7475377.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(26972..27664)
                     /locus_tag="ACHKW0_002561"
     CDS             complement(26972..27664)
                     /locus_tag="ACHKW0_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HFO7475378.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHKW0_002562"
     CDS             27794..29554
                     /gene="ycaO"
                     /locus_tag="ACHKW0_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory factor YcaO"
                     /protein_id="HFO7475379.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         7198 a         8057 c         7206 g         7190 t
ORIGIN      
        1 tcccctgcaa tattttgaat ttgcacgatt ttgtaggccg gataaggcgc tcgtgccgca
       61 tccggcataa acaaagcgca ctttgccaga aatccaggcc cgcattaaaa gcgggctttt
      121 ttatgtgcct taccagacgg gcatttcgtc ttgcaggaag ggattatgca ggcgttcata
      181 accaagtgtg gataatggtc cgtgacccgg aataaatgta acgtcatccc ccagtggcag
      241 caatttatct ttgattgaag aaatcagttg attatgatcg ccacgcggga agtcactgcg
      301 ccctactccg cctttgaaaa taacatcgcc agaaatcagc agctttgccc gatcatcaaa
      361 aaacacgaca tgacccggcg tatgcccagg gcaatgtaac acctgtaaag tcacattccc
      421 tatgctgatg gtatcgcctt cgttcagcca acgatctggc gtcagcggct ggcactcttc
      481 cagaccaaac atacgacttt gcgcaggcaa gccttgcagc cagaactcat cttctttttc
      541 cgggccaaaa accggcacgc cgtaatgttg cgccagttcc gccgctgcgc caacgtggtc
      601 cagatgacca tgcgtcagca ggatctgcat cagtgtcagg ccgctgtcat caacttcctg
      661 tttgattttt tccgcatcgc cgccaggatc gaccagtgcg gccagacggg tttgttcaca
      721 ccagattaat gaacagttct gggagaatgc ggtgaccgga ataatacgat agttcatact
      781 gcccctgttt cgttaagcaa ttactaccag tgccgtgctg gcccggtatc aatatgcaca
      841 aagttactac gtggataata tcctacacca cctgcgcgca tagataacgc ggctttgcga
      901 atattgctta acgcgatacc ttcaatatgg aaatccatcg cctggccttt agtgtgatag
      961 cttttcttcg ctactccacg gctgcgggcg cgtagttcat tgttggtatc aatagaacga
     1021 taaccggaaa tgagttgcac cggtttgcgc gtgcctaaca acccttgcag gcgatacaac
     1081 tggtcgaata atcctgggtc gatggacttt attttgttcg cgcggtaatc gcggaaaaaa
     1141 tggttaagtt ttgccaattc ttcctgaata tagcctctgc catcgaaaaa ctccgctttg
     1201 attgactctc cggtatgaag attattgagt gtcaaaatgc gcgggcgtgg ggtagagagt
     1261 gttgcaaacg caggggtcgg caggatggcg gcaccgagtg caacgccacc aagcgccagc
     1321 aatttgcggc gattagcgtc gaatttgtcc atgataatca agtctacagg tcaatgttat
     1381 cgtttatatg cacttctggc gcacaagagg caccttaacc gcccgtaaag cagacgtcaa
     1441 gatgactcaa ccccagcagt gacgggggct gcagagaatc gcccccaatc atgctaatta
     1501 ttacgacaac tgatttcccc gaactacttc atttacctga ttaattgttc cgctttcgat
     1561 acgatttgcg agctggatcg cgcaggcaga tcataattgt aaatatctgt acgatactgg
     1621 gtacgaccat ctgcaccaac aaaggccgtc aggtagtaga gattcaccgg aatcgactgc
     1681 cgaatattga cgtaacgtgt atcaccctgc ttcagcgcat cagaaatacg tttgtcattc
     1741 cagcctgcat cctgcaacag catattcgcc agatcggaag ctttattcac tcgtacacag
     1801 cctgagctca atgcgcgtgt atcacgcttg aacagattgt ggttcggcgt gtcatgcaaa
     1861 taaatggcct ctgaactcgg catattgaat ttatagcgcc ccagcgagtt ccgtgggcct
     1921 ggagcctgct ggaagcggaa cggtaaattc gaggccgtga ttgtagacca gtcaacctgc
     1981 catgggtcaa tcgcttctct gctgttccag ccgcgcatca ccgtatagcc atggctttcg
     2041 agatatcccg gatcgttgcg cacttttggc agaatatctt tgcgtgccag agttggaggt
     2101 acgttccacg gcgggtttac cactacgttg ttaagggcac tgctcatcat cggcgttttg
     2161 cgatcggggc gaccgacaat gactcgcgaa tccagcacct gattgccgtt ctgatagtag
     2221 accagcgaat aggccggaat gttaaccatg atcccggtag aaagctctgt tggcagcaag
     2281 cgcaatcgct ggatgttgag agccaacacg ccagcacgct gggcgggcgt tacgtttaac
     2341 cagtcacgtg ttgccgggcc aatagcacca tctgccccca atccttgcca tgcctgaaaa
     2401 cgtttaacgg cttccaccag ttcattatcg taggcggcgc gaacggcagg cgcaggttta
     2461 ctacgagatg tcgtttgctt gtccatcggc ttagtttctg ctgtttcaac agtaacagcg
     2521 gatgggctga ctaccgcgtc agttggcgtg tcatcgccag gtagagtaat tttcggcccc
     2581 ccgtccaaca tgcctgtgcg ttgcaatatt tcgcgcaacg ccggtacgtc gttactccac
     2641 tgccctgggc gcaacgttgc tttgccggtc agttggggcc acggtttggt gtcactgagt
     2701 aaggccagta acgattcatg catcgccgca tattgcggat gctgcggtgc cagtccagca
     2761 acaaacgtag gcaattgacc tttatccagc gccagctgcc attggttaat caccgagagc
     2821 ggcggcgttg caagtgcata aggtttactg ctatatagcc agcgagtgcc tttgaccgga
     2881 atatttgcaa tgaaatggag atagcccatc atcgcatcag agagcactac gtcgcgtgcc
     2941 atcccgttaa caccaggatc ggtcagtaac tctacccatt tattaaactg cggctggaaa
     3001 ccggcaatcg ccacctctgc cagctgttgc tggaaggctt taacagcatc acggttttcc
     3061 cacatgggtt gcatatcgcg tgcggcatac aacagttgaa gctggttaag ataaaccggc
     3121 ttgtaacctg cgggtaattg agattcgatt tgcgtgcggg ctttttctgc cgcaccttca
     3181 ggcaatggct ggatccccgc cattattgcc gtggcttgcg cctgcggcag tgcctcgccc
     3241 tgttcactga cagccaccgg gctgtcgcca gggattactt caggctcatc ggcctgcgca
     3301 ttgaacagtg gagcgaatgt tacggccagg cacaaactga ttgccgacag ccgacgacca
     3361 cacatcatat taagcaacat cccttgcccc ctgtttttat catactctga atagccaggc
     3421 tgttatttta cgacttgcct gtacaacgcc aatactcacg aaagtatgac gagcaagcct
     3481 gttactcatc aggcttatct tcagtatata aacgccgata cgtttttaca taacctaatg
     3541 taaagaagtt taaagaaaag cgcgccgtaa tgtgccactt ttttagtgca gaaacggagt
     3601 tttcggaaaa agaaaaggcg gcattgctgc cgccttaatt ttaactcgcc tgagaaggcg
     3661 cttcgtcact tcctggaagc gtttcaggga gttgcggcgc aaatcctcgc aggccgacga
     3721 cgtgaacgtg ttcggtattc tggaagactt tacgcaccag tttataggtg gtgcctttct
     3781 ccgggctgat attttccggc gctgcgatga tgagctgcat ttgcaaacgt tcacacaatt
     3841 caaacagcgt ggcgatagaa cgagcatcca gtcgcgctgc ttcatcgagg aacagcaggc
     3901 ggcaaggaga gatatcttta ccgcgcaggc ggcgagattc atcttcccag ctttgtacca
     3961 ccatcaccag aatcgacata ccggtaccaa tcgcctcacc ggtcgacaat gcaccagact
     4021 ctgcgcgcaa ccagccatcg gaaccacggt taacctcaac ttccatttcc agatagttgc
     4081 ggtaatccag cagttcttcg ccaatggtct gcggcgcgcg ctgccccata tcaatctgcg
     4141 gattaagacg ctgatacaat ttcgccagcg cttccgagaa ggtcagacgg ttgctgttaa
     4201 acagatcctg atgctgctcg tgctgttcag agagcacatc cagcagcatg gcgtgcgttt
     4261 cacgcacgtt cacgttcaga cgtacgctgt tcacctgacc aaacgatacg ttctgcaacc
     4321 cctggttgag catacggata cggttctgct cgcgctgaat ggttttgcga atgatgttcg
     4381 ccacgctgcg ggaactgatc gccagtttct gttcgcggga ggttaactcc tcggtcagtc
     4441 ggctaagttc gatttccatc tgctcgatgg cttccaccgg atcatcggta cgaataatat
     4501 cctgacgaat acgttcacgc agatgctgat aaaccgccac gaagaactga attttacgtt
     4561 ccggacgttt tggatcttcc gacatgcgca gcacgtcgcg cagatgttcg ttatccgcca
     4621 ccgccagacg cagcgcaccc aatgccttat ccgacataga gcgcaaatca tcagcggaga
     4681 gataagccag ctcgcgacgg tgtaagcgcc gttcaacgcc gttatctttc accatgcgca
     4741 tcactgcaca ccagcccgct ttggcggtca ctacctgctc gcgcatctca aagtaatccc
     4801 gctccagctt gcgcagtttg cgggtcagat tgtccatctc cgcttcgcag aaagtcagtg
     4861 ctttttccag ctggttgcgg cgggaccggt tattactcag ttgcgcatgc agctcgtcac
     4921 ggcgaattcg cgcccgctct tctgccccgc tatcggcgcg cacgccgata tcctgcaatt
     4981 cacgttgcag atcgttgagt agctcttttt tggtgtcgta agaacttttc agcgaagcca
     5041 gcacctggtt gtactgactc aactgcgctg cgtgaccacg caacgcttca cgagcgcgag
     5101 tacgctccgc ttccgcctgt tccagacgtt cacgcagctt ttcgttgaga tcgctattac
     5161 cgctcagcat ttctgccgag tcagaatagc taaagtgcgc acgacgctgc accacttccg
     5221 tcagggcaaa cgcctgctga cgggcatcgc gctgcatctg ctgagagtac gcgtaatctt
     5281 cttttagctg ttcgaactgt tccgggtcgc tctgcaatac cgaaacgatc ggttccagtt
     5341 tcgccagttg attgccaaac tgctgaacaa aacgcgcggc ttcctgggct tcatccagac
     5401 gttcgcggat ttcatcgacg cgatccgcca ggctgtcatc agccaacagg ttgagacgcg
     5461 gcagaatgcg gttcagcgcc gtaacgcctt ctttcgcctg ctcaaactga atacgctgct
     5521 gctggttatc attttcatga ttactcaacg cccgctccag ttcgacgcga cggctgttca
     5581 gttgacggat ttctgcttcc gggtcagact caaacgcaac cgccagatga ctgccgataa
     5641 agcggctgaa cgcctgatgc agacgctgag ttttctgtac atcaaaggag agcgtggcga
     5701 agcgttcgga aagcacttca cgctcggcat ggaggctttc aatacggctt tcacgcgcag
     5761 cacgaccaaa cagcggcact tccgggaaac gtgaataacg ccactgacga tcggcgattt
     5821 taaccactac cgctttttcc agctcatcaa cgctgaacac gctgtcatcg aatgactgcg
     5881 gatccccttc gatcaaatag agatcttccg ggcaatcggt caagccttcc aggtgttcag
     5941 ttacctgtga cagatctggc accacgatgg cgtggcgtga cgggccatac agcgctgaga
     6001 agtacggcgc atcttccagg ctaacgtcgt cataaatttc tgacagcagc acgccgccaa
     6061 aacgctccgc cagcgcgttc agacgctgat cttcagagcc gccaggctgg cttaaacgtt
     6121 cgatctcttc atcgacagcg tttttgcgcg cgcccacttc atcgcgttca acaatcgcct
     6181 ctcgctcacg ctccagcaac tgttgcagat attcggtgac gtcctggctg gaggtaaact
     6241 cttcaccgca ctgctcgctc aactggttga gactgttttg cgctgccagc caaaccggcg
     6301 cacgctgcat caaactctga atgcgagact gcagctgttc ctgttcctgg cgcagtgcca
     6361 tgcgctcttc acgggcgtta gacacgctat cggaaagaga ggcaatgcgc gcttccagct
     6421 cctgatgcag agcttccagt tcatcaatat caaaattctt gccctgacgt ttgcagaagt
     6481 ctgccagcag gcgttcggct tcttgctgct cgcgcagacg ctgttccagt tcacttaagc
     6541 gcatccgcaa cggttgaacc tgctctgcca ggtgacgctg atcgacccct tcgcgcagca
     6601 gttcgcgggc gacatcccaa gcctcgttac gcgccagcgg gccgttgatt gccaccacca
     6661 gctgataagc ctgctcaaac tggctgtgcg cggtttgcgc catgctcatt ttctgctcaa
     6721 gagagagcat tttttcagtc gcttccagct ctttcgcctg gaaggtttcc agccattcgg
     6781 cggcgctgtc ggcggttaag tccggcagat ggcacagttc tttggcgcga ttaagcgcag
     6841 caatcgcctg gttatactgg atcgcgcgcg tttgctggac gtccagcgcc tgctggtagt
     6901 cagcaagctg gcttttcagc tcgtccactt ccagttcggc agcttccgca cgagcctcgt
     6961 tctcttcctg gcgttcgatg gcttctgcca ccacttcgtt ttgctcttcc agacggatct
     7021 gcagctcatc gagatccgct tcgtagcgtt cgatcttctc ctgctgacgc agtgcggttt
     7081 gcaccaggtt caggtgatcg ctggccgcct gataatccgc ttccagatcg ccttcggcac
     7141 cgttgtgctc tgccagctca cgcgccatat cgacgtgttt gtactgctca gccgccagtt
     7201 gctgacgcga agtatgtagc tcgcgacgaa actccagggc tttgtcgaga tggacacggc
     7261 gctcgttggc gtgacgcatg tagtccgccg ccacgtagtt ggtagcttcg ctgatcagat
     7321 gcttaaacag gtcacggtcc gactgggtga cacgaatcgc ttccagcgtc atgcggtttt
     7381 cacgcagcgc cgcttccatg tcctggaacg ctttacgcac gccgctgttt tccggcaaca
     7441 ggtagtcgcg cagagaacgg gtaatggcac tggagatccc gccatacagc gaagcttcga
     7501 tcagacgata gaatttgcta cggtcagatg cggagcgcag acgacgcgcg atgatgccca
     7561 gatcgaacat cagcgagtgg taatcggtaa tggagttgaa ctgtttaaac tgcacgcctt
     7621 ccatcgcctc gagcttgtct ttcagctcgt taagcggcag cacgcgcgcc tggcgttcgt
     7681 tcagggtttc ggtcaccagc tgcgtcggca gcaccgacat cggcagtccc tgaatggcaa
     7741 atggtttgat atccacttta cgatcgcgtc cggcaacctg ttgcagacgc acaccgacca
     7801 ccacgcgctg gtggcgcgag ttaatggtgt cgagcatcga ataacagaca cccgctttca
     7861 gcttaccgtg cagaccttta tcgcgcgaac cgctggtggc cccggcttcc gtagtgttac
     7921 ggaaatgcag cagggtcagg tcggggatca gcgccgtaac gaacgccgcc atagtggtgg
     7981 atttacccgc cccgttaccg ccagaaagcg tcgtgaccag ctcgtcaagg tcaaaagttc
     8041 gggcaaaaaa gccgttccag ttaatcagcg tcagtgagcg aaatttaccg cgttcaatca
     8101 ttattcttcc tctccgctat ctggctgatt ctcttcgttt tcatcgttga gttgcagatg
     8161 attttcaatc ggcattgctt cgccatcacg aatcaggcga cgctgtgctt cacggggatc
     8221 gtcgccagca cgcacatcgg ctccgaagcg gaacaccgat tcggtaatgc ggaacttgct
     8281 gctgtcgtgg cccataaacc acaccatgcc taaacgacgc agacggttga gcgaagaacg
     8341 tactttctcc tgcaacttct gacggtcaac gtctgaaccg gttgaacggt tgttcaccag
     8401 tttcagcagt ttcgcttcat cggccagggt gagcagttcg tcgtacagtt cctgctgggt
     8461 gaaaatcccc tcattcgcca gccgttccgg actgagatag agataacaga ggattttccc
     8521 gaccatcata tccagttccg acaagacgga acgagggatc agcgtggtgg aacgtgggcg
     8581 taaatagaag aacccttctg gtgcgcgaat aagctcaacg ttataacgcg cgtaaaactc
     8641 ttccagatat tcctgaaaat ccatcaggaa tgcatgatta tccagttcgt cgaggccaat
     8701 atggcggcct gaacgtaagg cgctgtccag cgccggaaat aacggattcg ccagcgcctg
     8761 cgccagctta accggcatca cttgttcaat atttgtcgat gacatgcgcc tgtaccttgg
     8821 ctccgtaatc attaatcggc tgccatttcg ctggcagccc ggtgaaatct gcttgcgcta
     8881 cgccaagacg taccgcctga tcaataacaa tgcgcgcaac gtcaaagtga cgtgcacgcg
     8941 gatactgtga cagatattcg cgtaccacca gaccaagatc cagcggcact tgtctggttt
     9001 tgtacacagc aagttgttct tcgatgatcg ccgccagctg ttcgcggatc tcgttaaact
     9061 cttcgtattc taaatctggc ggaagttccc cagtcacttc ttcatcgcgc agtgccatct
     9121 cttcgtcacg catatccagc agacgatcgg cattggcata agttagcgcc cacggctcat
     9181 caaaataggt ttgtaccgac tgacgtaaac gctgggcaaa gacgcggttt ttatccatat
     9241 cgatcgcagt acgaataaat ttgtgtacgt ggcgatcgta gccaatccac aagtcgatgg
     9301 attgctggcc ccagctgata atacggtcga gtttgctctg cagatcgaac accagacgat
     9361 cgacaaaatg caggtcgtca tgggtcatcg tcgcatcctg aatgcgcaac agattagcct
     9421 gcaacttgtc gcctgccgct tccagcgtat cctgcaattc acgcagcgtt ccggaagttt
     9481 ctgaaagcaa caattcacag ctggaaatcg ccgcccgcca gtctttgttc agcaactggg
     9541 caatatcgtc cttcacctgc tgctgctgtt cgtccatcag acgttgcgtc aggtcgatac
     9601 tgtcgaaaat ttctgctacc gaatatttca gtggcgcata gacattacgg tgccagtgaa
     9661 attcatcacc gccctcttcg gcggcatcag ctgcgcgttt gagttcaccc gccacaatcg
     9721 acaactgcat agaaagacgc agcgtagaaa actcgcgctg acggatgtag tagtcagtaa
     9781 tgccgatgcc gagcggcgtc agacggtaaa ttgcgttccc ttccgcctgc tcgctggtaa
     9841 agcggttcag cagacgttga cgcaccatgt cgttgatcgc gttattggcg cgcacgccga
     9901 tggtttcgct ggtttgctca aacgcatcac tcacatggcg gaatgcatcc accagctcgc
     9961 cttcactcat ctcaccatcc agacgctcgc cgttcagcgt ggcaaccgcc agcagaaaag
    10021 agagtcggtc taccggcagc gagatggaga agtcattttt tctggcccag gcaaccagtt
    10081 cggggactgt ctgggaaaat tcactcatac tttatccttg ctctgcggtt tgcgcgcggt
    10141 gacatgaata taacgcccca gggtaatata cggttcctga cggcaataac gcgtttctaa
    10201 ttcaagtaat gcttcatagc agtcgcgctg ctggtgcttc tcgcgcagat aatcatgaaa
    10261 cacgcgaacg cctgtcttac ccataatttg ccaaccagct tcttccagcc acagataaac
    10321 ctgcgccggg tcgcgtggat aatctggcga aagcgtccgt tttttctttt tcggcattcc
    10381 cgcctgcacg taatcaaaat tcccggcgac catgttatgc atcaacaaac catgcgcatt
    10441 gtagaacatt aacgacaaca cgccgcctgg acgcaacact gaccacaggg tctgcaatac
    10501 actgcgggga tcggccaccc actcgagcac cgcatggaac aatatcagat caacgggcgt
    10561 ttccaaatgc gaagcaacat cctgagcggc gcaatgtata aattgcatgt tgtcgctcac
    10621 accttttgct tctgccgcct gttttgcgcg gtcgatcatc tgcgcagaaa gatcgcataa
    10681 aatgacctga tgcccacgct cggccatttt gattgcggtc tgcccttctc caccgccagc
    10741 atccagcaca cgcagttttt gcgggcccat ttccgccagc acgcgatcga gatcctgcca
    10801 cagaatagcc tgtcgaagct gccctttggt ggtgccgtaa atgttacggg aaaacttttc
    10861 cgcaatatca tcaaaattgc gatcctgcat tgctttctcc accggttggc taagacgttt
    10921 tctgataaag acgctatttt gtcacagcca cggcgagaat gaacccgtct ggtgtaatat
    10981 cgctggcgat cccttgctat atggttaaaa aaggaaccag aaaggatgct ttttacactg
    11041 aaaaaagtga ttggcaatat gttgctgcca cttccgctga tgttgttaat tattggcgct
    11101 ggcctggcgc tgctgtggtt tagccgtttt caaaaaaccg ggaagatttt tattagcatt
    11161 gggtggctgg cactcttgtt attgagtttg caaccggtag ccgaccgcct gctgcgtccc
    11221 atcgaaagca cctatccgac atggaataat tcgcaaaaag tggactatat cgtggtgctc
    11281 ggaggtggtt atacctggaa cccgcagtgg gcaccgagct ctaatttaat caataacagt
    11341 ctgccacgcc tgaatgaagg tattcgctta tggcgcgaaa atccgggatc aaaactgatc
    11401 ttcacaggag gcgtagcaaa aaccaatacg gtgagtacag cggaagtagg tgccagagtt
    11461 gcgcaatcgc tgggcgtgcc gcgcgagcaa attatcaccc tggatttgcc aaaagatacc
    11521 gaagaagaag ctgcggcagt gaaacaggcg attggtgacg cccctttcct gctggtgacc
    11581 tccgcatcac atctgccgcg ggcgatgatc ttttttcagc aggaagggtt aaatccgcta
    11641 ccagccccgg cgaatcagtt ggccatcgac tcgccgctta acccgtggga acgggcaatc
    11701 ccttctccgg tctggctgat gcatagcgat cgtgttggct atgaaacgct tggccgcatc
    11761 tggcagtggc tgaaaggtcc gtcaggcgag ccacggcaag agtgattttg atgccagatc
    11821 gaagttgcgc cgactaaatc gtccggtatt aaccagttgc gcaacttcat cccataacac
    11881 atacaaccaa cgccgccaga tgaacgactc cgccactggc gcgcgttgca ggtaactcca
    11941 gagcaaatct tccgccagag aattatccat tagtcggaac agttcgtatt ctcgcggtgc
    12001 ccaaagcatt agtcccggcc cgaccatcgc cagtaactga tcgctgcgcg aatctttcag
    12061 catgctgcgt aaacagaagt taccgtgaat caacacacaa ttgtcgttaa agccttcaaa
    12121 taatgccggg agacattcgc gagtgcgaaa caggatccgc ttatcctgca tcgtcagacc
    12181 ggtattattg aactgattga gcgtggtcca tagcacttca acatgttgca ggtaccatga
    12241 gggccagaaa ttttcctgag tattgtcgac cgcgccgacg caaccgcgac tgtcctgacg
    12301 gtgccaggcc agtaaggctt caacgatttg gtctttgagc tgttcccagc gttctggtgt
    12361 tcgggctggt gcctccaccg aaacaccacg catccgctcc agtagcagga catcagggcc
    12421 ggggtgttct tcatgcgtca tcacaccata aatagtcggc atacggacag tcccacttct
    12481 tgccagcatg gtggttttcc atgccagttg tcgggctttt ccgggcgtac taaagcttct
    12541 tgccattaac ggcattgggt ttccctggct gtcatacaaa gcccacaacg ccgtatccgc
    12601 tttttcattg acgcactcaa tacgactgag tttttcgccc agtaaatggc ttaattcggc
    12661 acgcagctgt tccattcaga ttaccctcat aaaagctact gctttcataa tgagcgccaa
    12721 tataaaagat gtcacctgaa tgagatcaat tgttgaaaag aaaaattgag gaaaatcgta
    12781 acggtacgac actcctccca aaattggctg aagtgtcgtg aagtgaaatt agcgcatttc
    12841 agcgcgaacg cgctcaagat cttcaggggt atccacacct gtgccaggaa cttcctgagc
    12901 aacagcaaca tggatttttt cgccgtacca cagaacacga agctgctcta acatttcgat
    12961 gtgttctaac gggcttggct gccaggtgac gtaacgacgg ataaagcctg cacggtagcc
    13021 ataaatacca agatgacgca ggaagttatc gccaacggtt tcaaggtctt ttgcaaaacg
    13081 gtcacgatcc cacggaatgg tggcgcgtga gaagtacaga gcataccctt cagcgtcgag
    13141 aacgactttc accgcattcg ggttaaacgc ttcttccgca ttgtggattg gcgccgccag
    13201 agtcgccata cccacctgac gctgagcgag gttatcagca acctgacgaa tgatggtcgc
    13261 agggatcatc ggttcatcac cctgcacatt aacgatcacc gtgtcgtcgc tgaatgcgca
    13321 tttttcgaca acttccgcca gacgttctgt tcctgactga tgatcggcgc gcgtcataca
    13381 tacttcaccg ccagcggctt caacggcgcg ggcaacatcc tcatgatcgg ttgccacgat
    13441 gatgcgctcg gcacctgatt cacgcgcgcg ttcaagaaca tgaacaatca tgggtttgcc
    13501 gttaatatca accaatggtt taccgggcag acgcgtggac gcgtagcgcg cgggaataat
    13561 gaccacaaaa ctcatgattt actctcatca gcagtcagca cgcgggcttc cgtttccagc
    13621 aacactggaa tgccatcgcg gagcgggaaa gccaggttat ccagtttgca aataagctct
    13681 tgtttttctt ggttatacca aagttttccg ttgcaaaccg ggcaggcaat gatttcaagc
    13741 aaacgatgat ccatagttcc tccagatgga tcgggttatg aatgcataaa tcttatcata
    13801 atcatttatg cgacggggtc tatttcccag ccagtgcggc aatcagtaaa gagaccttgc
    13861 gggcagcgtc ctaatgtcac ccgcgttgcc tgctgccagc tcgcgaaatc agtaatcgcc
    13921 tgacgtaacc ctttttgcag cattgtcgtt ggtttaatgc cttcctgtaa ccacagagag
    13981 ataacttcaa ggatgcctgt ctggcgatgc attttggcat ccattcgccc aactaattgc
    14041 ccacgatgta ataatggcag aacaaaatag ccatactggc gtttcggcgc tggggtatag
    14101 cactccagcc ggtagctaaa atcaaaaagc tactctgcgc gtttgcgatc ccagacaaca
    14161 ggatcaaaag gcgaaagtac cgcgctgtgc gttgcagtga gctttccggc cagcgcacgc
    14221 tcgagtagcg gcagcaaatc agcatgcagc caaagattgc ccaatttttc aacatgcaca
    14281 gcaatgattt gctgctgttc agccctcgct tcgcgccacg ccgccagtgc cggacgtttc
    14341 agccgatagt aatctgccag ccactgttcg cggaatattc ccaggctacg cgcactgtta
    14401 tccagcatga tgatttctgc ttctgtttgc gaaacgagat cgcgctcatc atcccagtca
    14461 ggcatgacac ggtgggttaa atcataaacg cgctggaagt tgcgccgttc aatcaccatc
    14521 acctttccgg cagtaaataa accttccaga tgccgtttat gcggcttcca ttcccaccag
    14581 ccgcttgcac ctttacgagg atgctcaaaa tcggctgaac gtaccggccc cttatcatga
    14641 atatgctgaa ttaactgtgc aatttccgcc tcatgttcct gcatccaggc gtctttgtat
    14701 ttccagccca ttttttcagg tgccagcatg cggtggcgaa taagacgaaa gtcgctacgc
    14761 ggcataaagc aggcttcatg cgcccagtat tccattaatt cgccacgcgc cagagactca
    14821 tccagccact gggcaggata atttcccaga cgactgaaaa gcaccagata tggactacgg
    14881 gcaacaatat tgatggtatc gatttgcagc aaggacatgc gggagatcgt tgccggaata
    14941 tcctccaacg acgcttgacg gcggggtttg tttaacaggc cttgtgcggc aaggtgaaga
    15001 ttacgcgcat cagcaaggga gaggtgcggc agcgacattc atgactccat caatcgaacg
    15061 ctgccgcggc gtaactagtt gccagaagcc agcgaggtta gttgcgtaag cagtttcgct
    15121 ggttcatcac ctgaaagctg tgcgtctaca ggcaaatacc accaattttc ttctgcaaag
    15181 gcccggcatt tcaccgcatc tttttcagtc attaccagcg tttgcccggc gcttaccaac
    15241 gcactgacat ccgcatggtt caaagactga tgatcggcca gcggtacaca tttttccggt
    15301 tgtacgccac acatcttcag cgtggcaaaa aagcgcggcg gatgcccaat ccccgccatc
    15361 gccactacat gttcaagctg agcaacgtca caacgcgtac cggtacgtaa attcaccgcc
    15421 tgacccggca gcagatgcat ggggatttca ccgctgcgag ggacaccgcc gttgacgatt
    15481 accgcatcaa ccgactttaa gcgccccgct cgctcacgca ttggccccgc cggcaaccac
    15541 cagccattgc caaagcgacg cacaccatca ataacgacaa tttccacatc acgcgccaga
    15601 cggtaatgct gtaaaccgtc gtcggttacg atgatctgca catcagggtg ttgcgccaga
    15661 atggctttta ccgcatcaga acgaacggga gaaaccgcaa caggcgcatc agtgcgttga
    15721 taaatcaaca caggttcatc acccgcctgt gctgttgtgg tatctgccga caataacagc
    15781 ggataagatt cagccttacc actatatccc cgcgatacga cccccacgcg aataccgcgc
    15841 tgttgcaact gttccaccag ccagacaacg accggggttt ttccgttgcc gcctgcggtg
    15901 agattaccaa ccacgacaac cggtacgggg gcacgccagg cgcgcttcag ttttagttta
    15961 tagcaaagac ggatcgcgcc acttaccagg ccatacaacc aggagagtgg cagcaatagc
    16021 cgccacaaag gggattcacc agaccagatt ttttcgatca ttggccaaac tgcattttgt
    16081 gaagttgcgc gtaaacgcca cggtgctcaa gtaaatcgtt atgcgtaccg cgttccacaa
    16141 tgacaccatc ctcgacgacc acgatttcgt ctgccttttc aatggtagac aagcggtggg
    16201 caatcaccag agaggtacgg tttttctgca actcatccag tgccgcctga atcgcacgtt
    16261 cggattcggt atccagagcc gaggtagctt cgtccagaat cagaatcggg ctatcacgca
    16321 acaaggctcg agcgatagca atacgctgac gctgaccgcc agagagcagc acgccgtttt
    16381 caccaatcac tgtatcgaga ccgttatcca tcttattgat gaagtccata gcgtaggcca
    16441 tacgcgccgc ttcttcaatt tgctcacggc tgtactgttc agttcgtgcg taagcaatgt
    16501 tgttagcaac cgtatcgtta aacagatgga cattctgcga caccagagca acctggttac
    16561 gcaacgacgc cagggtatac tcgcgcagat cgtgaccatc catcaggatt tcgccttcat
    16621 caatatcgta aaaacgcgtg atcaggctgg cgatggttga tttacctgaa ccagagcgtc
    16681 caaccagagc aaccgtcttc cctgccggaa ttttcaggtt gatgttacgc aatgcaggta
    16741 cgtcacgtcc cggataagta aaggtgacgt tgcggaattc cacgtcacct gtcgcacgct
    16801 cgatcacgcg cttaccttcg tctttctcct gctcactgtc cagaatggta aacagcgtct
    16861 gacaagccgc cataccgcgc tggaactggg cgttaacgtt ggtcagcgat ttcagcggac
    16921 gcataagtgc aatcattgaa gagaaaacaa cggtaatcgt accggcagtc aggctatcca
    16981 tgacacttgg gaagcttgcc gcatacagaa caaacgccag cgccaaagag gcgatcagct
    17041 gaatgatcgg atcagagatg gaagaggctg aaaccatttt catcccctga agacgcattc
    17101 ggttgctgac tttatcgaag cgtttcgttt ccacttcctg accaccgaaa atcaatactt
    17161 ctttgtggcc ctttagcatt tgttcggcgc tggtggtcac ctgccccatg gtgttctgca
    17221 tgtttttact gatgttgcga aaacgcttcg atacaacgcg aatcgcaatc gaaacaatcg
    17281 gtgccagcac aatcaaaatg atcgacagtt gccaactgta atagaacatc atgatgaaca
    17341 ggccgatgat tgacgcacct tcacgcacaa ccgttatcag tgcgccggaa gaagaagaag
    17401 caacctgttc ggaatcgtag gtgatacgtg acaacagcgt acccgttgac tgtttgtcaa
    17461 aaaaggaaac cggcattccc atcatgtgat caaacaggcg gcgacgcatg gtcattacca
    17521 cctttcctga tacccaggag atacagtagc tggagacata gctggtgata ccacgtaaaa
    17581 tcatcagccc gatcaccacc agcggcatcc acaccagcac ggagcgatct gttttaccaa
    17641 agccatcatc aagaagtggc ttaaggagcg ataacatgaa ggtatcgctg gctgcgttga
    17701 ggattaacgc tacgcccgcc acgatcagac ccgctttaaa aggcgcaatg gttggccaca
    17761 gtcggcggaa cgtctgccac gtagagagat ctttgtcgtt atgcattcaa aaaaccagca
    17821 tttgttgaaa tagccgcata ttctacccgt tatccactgg cacgccaaac cactgatgat
    17881 accaacgagg taaaatttgc tctctgagac tgctaatccg ccagccttgc gctgaaaaat
    17941 tgaccgttac ttgcccctga tgaggagtat cgagccacgt ataaccctgt tgttgatagc
    18001 gatgcttaac tttgttagag ggcagtcgcc atgcgttata gcgcgatgcc gatgcgagtg
    18061 ccacttttcc attcactcgc tgaattaatg gcaacgatga tgaggtatta ctgccatggt
    18121 gaggtacctg aagcaatgtt gcctgcactt gctgccagta acggcttagc atcttttgtt
    18181 cagcggggac ttcaatatca ccggttagaa gaatgctatt catcccgtca tcaaccttaa
    18241 ccacacagga atggttattt cctttatcgt tgctagcttg taaaggccag tgcacgctga
    18301 aacgcaatcc ttgccattgc cacgcttcgc cacgcacaca gggctgatga tgttcccagt
    18361 ttaacggact tctgatccat aacatcggcc atgtatgcaa tattgagtcc agccctcccc
    18421 ggtgatccag atgttcatga ctcagaataa cgccttccgg ttcaagatta tgccagtgga
    18481 gccaggggat aatcagttgt tgcccactat ccccttcagg ccaggccagt cccgtgtcat
    18541 agagaatcgc tttgccgttt ctggctatca ccattgccag cccttgcccg acatcaagca
    18601 tgtacacttg ccactcgtca ggtcgaggtt tttgccacag cggccagcac atcagtaagc
    18661 ctccagccac acacattgct ggcaacgttc gccaggcgtt taatcgccat accaccagta
    18721 agaaccatgg ggaaaatgat agccattgcc aacgttcagc aatgttgatc cacccttccg
    18781 gcaaactctt taacccccag aaaagtaaag ccaaagaccg gtcggcaaga aaccataacc
    18841 cttgctccag gattaacggc ccgcttaaat gcacaaccat cgcggcgagg atcaacggaa
    18901 ccgtgataaa tgtcaccaag ggaattgcaa atagatttgc aataaacgag gtcagactaa
    18961 tgccatgaaa tatgacgatt tgcacgggca taagcaggag tgtgattccc agttgcaaat
    19021 gaatgaggga aacaactgca cgcaataccg gcggcagttg ccactcagga cagggaaacc
    19081 actggtacca aaaaatcagt gccgcgaccg cagcggcaga gagccataaa ctttgcgaga
    19141 gaatggcaac aggatccatc agcaaaattg ccgccagaca acatatccat acatcccagc
    19201 cactccactg tcgcccactt aacttaagca ttccccacgt agcaagcgcc accacggtac
    19261 gcaatgcagg aggttgcatt cccgtcaacc aggcataaaa agcagcacag cagattctgc
    19321 caattaatgg tatttgccag tggatccagc gcccaggcag aaaaatttgt ccactgcgaa
    19381 tgagtccggc agccagcaac gccgcaaaag cgatgtgcaa tcccgatatc gccattaaat
    19441 gcgccgttcc agtatcacgc atgatatttt tgatttcttt agggacggat aaccgtaccc
    19501 ccatacctaa accaagaata accgcattcc acatataggg ttgcagcgtt gtttgtagtg
    19561 acgccagata ctgtgcccgc aggctacaat tcggttcaat gacacttgcc tgcagaaagc
    19621 ggccggtgag cggctgatgc tgggcaatgg cataacgctg gctatcaaag ccgccatcat
    19681 taagttgacc atgaatggca cgaactttga gtttcattga ccacagttgt ccagcacaaa
    19741 ccgcttgcgg aagatattcg ccatacagca cgaggcctgg cgcagggaat atacgtcgac
    19801 cttgtagatg agtgatttga ccgtaatgag tggtcatgcc gtcagtagta gtgatctcaa
    19861 caattgcatc ctgcgtcgca ccagttaagg tttctcctgc ccacaaaatt tgctttgccg
    19921 ctaatatacc ccaaacgaaa aacagcaacg tcagcgcgat ataacgtacg gttttaacag
    19981 ggataaatgc cagtacgcag gcgaagatag tcagaaacgc aagggttaat gtcccaggca
    20041 attggggcaa aatcagcaac ggaaaaattc cgcaaattat gcatacgccg actgtcgtta
    20101 ttttcatgca cccctccctg tttatggaaa gtgtgcaact ttgttgagcg gttgtctggt
    20161 gggaattttg cgttccccga ggcacgttca gaagaattta acgagagggt gaaaactgct
    20221 caaaactctg cgaagcagct tccagttcca gattagagaa atgaaaaaag cacccgacag
    20281 gtgcttttct ctcgttcaag tttgagtaaa aacttaaccg taaatattgg cgcgatcgcg
    20341 cagttcttta ccaggtttaa agtgaggaac gtattttcct tccagttcta ctttatcgcc
    20401 agtcttcgga ttacgtccgg tacgtggtgc gcggtagtgc aaagagaaac tgccgaaacc
    20461 gcggatttca atacgctcgc cctgcgcaag agtcgaggcc atatgctcca gcatctcttt
    20521 tactgcatct tcaaccgtct tggcgggaat gtgcgattgc tgggtggcaa gtctttctat
    20581 caattctgac ttggtcatga ttcctccggt tccttaaagg caaattaagc ggctgctgtt
    20641 gctgcattga ttaagggcgg ccgtagccgc ccttagtgct tgattacagg acgaaacctg
    20701 caatctgtca agtaaactca acaaacttcg gaataaaaat cccgaagagt cagagaatta
    20761 ctcgccttta gctgctttga aagcttcagc cattgcgttg ttggagaagt ttgcatcttc
    20821 ctgtttgtta acagttgcga ttgcatcttt ctcgtcagct tcgtctttcg cacgaacaga
    20881 caggctgatt gcgcggtttt tacgatcaac gccggtgaat ttagcttcaa cttcgtcgcc
    20941 aacgctcaga accagggtag cgtcttcaac gcggtcacgg gatgcttcag aagcacgcag
    21001 gtaaccttca acgccgtcag ccagttctac ggttgcgcct ttagcgtcaa ctgcagttac
    21061 tttaccggtt acgatagcgc ctttcttgtt cagagcaacc cagttgttga acggatcttc
    21121 tgcgagctgt ttaacgccca gggagatacg ttcacgttct gcgtcaacct gcagaacaac
    21181 tgcagcgatt tcgtcgcctt ttttgtattc acgaactgct tcttcgcctg caacgttcca
    21241 ggagatgtca gacaggtgaa ccaggccgtc gatgccgccg tccaggccga tgaagatacc
    21301 gaagtcagtg atagacttga ttttaccttc aacacggtcg cccttgttgt gggtttccgc
    21361 gaactgctgc cacgggttag ctttgcactg tttcagaccc agggagatac gacgacgttc
    21421 ttcgtcgata tccagaacca taacttccac tacatcgcca acgttaacaa ctttggacgg
    21481 gtggatgttt ttgttggtcc aatccatttc ggaaacgtgt accaggcctt caacgccttc
    21541 ttcgatttca acgaagcagc cgtagtcggt caggttggtc acgcgaccag tcagtttggt
    21601 accttccgga taacgtttag cgatagctac ccacggatct tcgcccagct gtttcaggcc
    21661 cagggataca cgggtacgtt cgcggtcgaa cttcagcact ttaacagtga tttcgtcgcc
    21721 cacgttgacg atttcgctcg gatgcttaac gcgtttccag gccatgtcag tgatgtgcag
    21781 caggccgtca acgccgccca gatcaacgaa tgcaccgtag tcagtgaggt tcttaacgat
    21841 acctttaact tccatgcctt cctgcaggtt ttccagcagc tgatcgcgct ctgcgctgtt
    21901 ttcggattcg ataacggcac gacgagaaac aacaacgttg ttgcgcttct gatccagctt
    21961 gattactttg aattcaagct ctttgccttc caggtgcaga gtgtcacgca ccggacgaac
    22021 gtctaccaga gaacctggca ggaacgcacg aataccgttc agctcaacag tgaagccgcc
    22081 cttaactttg ccgttgataa caccggtaac agtttcagca tcttcgtaag ctttttccag
    22141 cgtgatccag gcttcgtgac gtttagcttt ctcacgggac agcagagttt caccgaagcc
    22201 gtcttctact gcgtccagag caacgtcaac ttcgtcacct acctggattt ccagctcgcc
    22261 ctgggcgttt ttgaactgct cagccgggat ggcggactca gatttcagac cagcgtcaac
    22321 cagtactacg tctttgtcga tagcaacaac aacgccacga acgatagaac ccgggcgggt
    22381 ttcgatttct tttaaggact cttcaaagag ttgagcaaaa gattcagtca tgtttaatct
    22441 tcaggtttat atttaacgtc cacctggctc catgccggat ggggttgttt aacatacccg
    22501 ctgtcattcc attgcaacgg gggtactgca aattcggtcg cttatgcgag agccaatttc
    22561 tggcgcgcgt attgtagcgc tttttcaatc acttgctcaa tgcttaaggt ggtggaatcc
    22621 aacactaaag catcggctgc cggaaccagt ggcgctaccg ctcggttacg atcgcggtcg
    22681 tcgcgttctt tgatctcggc caaaaggcgc tcaaagttaa cactaaagcc cttctcctgc
    22741 aactgtagca tgcggcgatg cgcacgttct tccgaggagg cgtcaaggaa aattttcact
    22801 ggcgcatcag ggaataccac cgttcccatg tcgcggccat cggcaatcag gcctggtaat
    22861 tcgcgaaacg cgcgttggcg acgcaataat gcttcacgaa cgcgtgggaa tgccgcgact
    22921 tgtgaagctg cattcgccac ttcctgagta cgaatttcgc cgctgacatc ttccccttcg
    22981 aggatcactt ccagattgcc attggttgac acaaaacgta catccaggtg ggatgccagc
    23041 ggtaccagcg catcttccga tgcaacatca acatggtgat gtaatgccgc tagtgccagt
    23101 acgcgataaa ttgcacccga atccagcaga tgccattgca atgcttccgc catagcctta
    23161 cacaaggtgc ctttccctgc accgcttggg ccatcaatgg taataaccgg ggcaattgcc
    23221 gtcatcttta tctccttaaa acaggcgtac cgttaacata accgcgcgca ttatacgcgc
    23281 cgacgccaca aatcgttaac gttgtgtgca aaaaccagtt ttgtctgtat tcagatgggg
    23341 atttattgcg caattatagc gggctggcag aagaccagcc cgaaagatga caatgattta
    23401 cttaccagag gcgatacgat cacggatgtg ttgcgcacgc tctgttgatg gtgggtgaga
    23461 gtcaaacatg gactgggtgc gaccgccatc cagactagcc agtttttcaa agctgccaac
    23521 cagcccctgg gtgctgatgc cacgtttctt caacagatca aaggagaaat catctgcttc
    23581 ggactcttta tcacgggagt atttagcatt gatagcgcct tctgcgatat cacccaattg
    23641 tgagcgggaa agctgggaag ccacaccgct ggtagctgaa atggcatcgc gtgcggcaac
    23701 gatcgcataa gaagctttca tttcagccag cgagtgcccc aacgcgacgt ggcccagttc
    23761 atggcccaga acaccttcaa tttcgttgtc attcatcatg tccatcaggc cactgtagac
    23821 acgaacacag ccgttcgcca tcgcccatgc gttgacgtcg ctggtcatat aaaccttata
    23881 gttgaccggc gtgccattaa tattgttacc caatgctttg gcgatttttg ccagacgttt
    23941 accgtacttg ctggatttac ttgcgacttg attgccgctg tccatttcgg cacagccctg
    24001 attggcaatc gcttttgcat cggcatcgct taatgttgct gctttatagg catttaaacc
    24061 ggagctgata gccatattgg tatcaatacc gtgggtattt tgacaccctg tcagtaatgc
    24121 tgcagaggtc gcaatcgcca gcagtaattt agtattcttc atgtgaatca tccgttgatt
    24181 aatcatatat aaatattatg tatcaacaac ttacattgac aagcgacgct accattaatc
    24241 aaaatgactc acaaggtccg aaaaaaaacg ccgacaatgt gccgacgttt tgataaagaa
    24301 agatttggct atttattgcc cgttgttcat tcaggctgcc tggctaatcc gcgccaactg
    24361 ctcgaaataa tccggaaatg ttttggccgt acatttggga tcaagaatcg tcactggtgt
    24421 atctgacaac gccaccagcg agaaacacat cgccatccgg tgatcattgt atgtcgcgat
    24481 ctcggcaaag ttcagttttt ccggtggagt gatacgaatg taatcgtgcc cctcttctac
    24541 ttccgcaccg actttacgca gttctgttgc catcgcaaac aggcgatcgg tttctttaac
    24601 acgccagtta tagatattgc gcagcgtggt ggtgcctttt gcaaataacg ccgccgtggc
    24661 aatggtcatc gccgcatcgg gaatatggtt catatccata tcaatagcgt tcagttcacc
    24721 acgcgtgcag gaaatataat catcgcccca gcaaatggtc gcgcccattt tttccagcac
    24781 atcagcaaag cgaatatcac cctgcatact gttacgtcca ataccggtca cttttacagt
    24841 gccgcctttg attgctgctg ctgccagaaa gtaagaagcc gaagatgcat cgccttcgac
    24901 caaataagta cccggagact gataagactg cccgcctttt acgacaaatt gttgatagtg
    24961 ctgattttca atttcaacac caaacgtctt catcagattg agtgtgatgt cgatataagg
    25021 tttagaaacc agatcgcctt taatacgaat caccgtatct tccggcgcaa gaggcgcagt
    25081 cattaacagt gcggtgagga attggctgga aacggagcca tcaacgtcaa cgttgccgcc
    25141 agtaaagccg ccctgtaaac gcaacggcgg ataattttct tgttccaggt aagtgatctt
    25201 cgccccgccc aggcgcagcg catccaccag atgaccaatc gggcgttctt tcatacgcgg
    25261 ctcaccggtc agcacaatat cattgctacc cagacaaaga gctgccgcca gcggacgcat
    25321 tgccgttccg gcgttaccga ggaacaactc cagggcacct tctgcgtgta atggaccgcc
    25381 gttaccgata atttcgcaac gcgtacgatc ggctgaaagc gtatagctta cccctaacgc
    25441 tgttaatgca ttcagcatat ggcgcacgtc atcgctatcc agcagattgg ttaatactgt
    25501 tttgccgtgt gctaatgccg ccagcaataa agcgcggtta gaaacgctct tggaaccggg
    25561 cagattaata gtgccatcga cacgagcgat gggttgtaac gtcagggatt ccatgaactc
    25621 aactctctac aacagaaata aaaaccccac aggctggctg tggggattaa gcaaaatttc
    25681 ggcattaacc gtgacggcgt tcgaactcaa ccatgaagtc tgtcagcgct ttaacgcctt
    25741 ccagcggcat ggcgttataa atagaagcgc gcattccgcc gaccacacgg tgacctttca
    25801 gtgcatgaag gccagcagca aaagactctt caaggaacaa tttgtcaagc gcactgtccg
    25861 ccaactggaa cggcacgttc atccgcgaac ggttagcttt cgccacgtca ttgcggtaga
    25921 aatcgctgtt atcaatcacc ccatatagca gttctgcttt ttgctgattg attttatcca
    25981 tttcagctac accgccgttc gctttcagcc atttaaagac cagaccagat agataccagg
    26041 caaatgtcgg cggcgtgtta aacatggagc cgttatcgtt gaggatggaa taatccagaa
    26101 tcgacggaca cgcgatattc gctttgccca gcaaatcttc acgaacgatg acgattgtca
    26161 ggccagccgg gccgatattt ttctgcgcgc cagcgtaaat cacgccataa cggctgacgt
    26221 caatcggacg ggaaagaatg gttgaagaga agtcggcggc gaccaccaca tctttgccga
    26281 agtctggcgt ttcgtcgatg gcgataccgt cgatggtttc attcgggcag taatgcatat
    26341 aagcagcatt atcagagagt tgccattcac gcattggctt aaccgcgcgc agaccatcaa
    26401 cagtcacttt ggcgtcaaag acattaggcg tgcagtattt tttcgcttct ttaatggcac
    26461 ttgccgccca gtaaccggca tcaacataat ctgcggtggt tttatcaccg agaatattca
    26521 gcggtaccgc agcaaactga ccgcgaccac cgccatggca gaataatacc ttgtagttgg
    26581 aggggacatt aagaagatcg cgaaaatcct tctcggcttc ctctgcaacc tgaatgaact
    26641 ctttgccacg gtgactcact tccatcaccg acgtaccaag accgttccag tcgcgcagtt
    26701 cctgttgagc ctgtttaagc acctctgccg gtagcattgc cggaccagaa ctaaaattga
    26761 agatttgagc catttcccct caccacgttg cgttgctatt aacccgcggt caatcgattg
    26821 cgaccacagg taatacatta ttcctgtggc tatcggtttt atcattcagt gacacacatc
    26881 gcaatgagta aaaccgttga gggcaaaaat gtggcttgca tcacacaaaa gaatctctcg
    26941 gattattagc aaaaagccga tgtttttact gtcaggtcac ccgttttgcc cgacttgcct
    27001 tcgtccattc tggatttgca cagcgcggac ataattgttt ttcccccgct ttcatatgaa
    27061 tgatttcact acaacgaata caggcatagt ccgccgactc cggcaccact ttgtaacgct
    27121 gcgtacaatc actgggtaaa ataagtaaac ccggtttcac cttgtcatct tcaaagaaat
    27181 agacactgat ttgcccgttg gtttcgagaa tcgccagacg tacctgcccc agctgctcca
    27241 cgccacgcaa tcgtagctcc ataaagaact caaattccgt catgttggag ttattgagtt
    27301 tcgaccaggc cagctcgcca tcttcaataa taacaaccgg cttaccttcc agtagatctt
    27361 ccagtttttc actgtgcgcc atcaaccaca ttaccaggcg gtataacaac gccagagtga
    27421 taaagacgat aagtaccggc accatcggca catcatcata aaacgccaca tctcccgccg
    27481 ctgaacccag cgtgagaatg attaacactt caaacagcga catctgtcgc acaccgcgtc
    27541 ttccggtcat tttgaggaac aaaaaaacca gtacaaaggt ataaagacta cgtagtgcaa
    27601 cttcgccaag gaaatcaaaa ggcactttat caaatgccat acggtggaga tcgaacgcct
    27661 tcatttttat gctcttatcg tggtttatcc gctaaaagca tagtcaacag ttcatcggca
    27721 tggcggcggt gatagcgcct ttgagataaa acccgtatta ttgcgcgctt tccgtacgac
    27781 taaagtgatt ttcatgacgc aaacatttat ccccggcaaa gatgccgctc tggaagattc
    27841 catcgctcgc ttccagcaaa aactttcaga cctcggcttt cagattgaag aggcctcctg
    27901 gctgaatcct gtgcctaacg tctggtctgt acatattcgc gacaaagagt gcgcactgtg
    27961 ttttaccaac ggaaaaggcg caaccaaaaa agcggcgctg gcttctgcac ttggtgaata
    28021 tttcgagcgt ctctcaacca actacttttt tgctgatttc tggctgggcg aaaccatcgc
    28081 caacggtccg ttcgtgcatt atcccaacga aaaatggttc ccactgaccg aaaatgacga
    28141 tgtgccagaa ggactactcg atgaccgtct gcgcgcgttt tatgatccgg agaatgaact
    28201 gaccggcagc atgctgattg acctacaatc cggtaacgaa gatcgtggta tttgcggcct
    28261 gccgtttacg cgtcagtccg acaatcagac cgtttatatt ccgatgaata tcattggtaa
    28321 cctgtacgtc tccaacggta tgtccgcagg taatacccgc aacgaagcac gcgttcaggg
    28381 attgtctgaa gttttcgaac gctacgtgaa aaaccgcatt attgctgaaa gcatcagcct
    28441 gccagagatc ccggcagacg tgctggcgcg ttacccagca gtagttgaag ccatcgaaac
    28501 gctggaagcg gagggtttcc caatcttcgc ttatgacggt tcgcttggcg gccagtatcc
    28561 ggtgatttgc gtggtactgt tcaatcctgc taacggtacc tgctttgcct ctttcggtgc
    28621 gcatcctgat tttggcgtag cactggaacg taccgtgacc gagctgctgc aaggtcgtgg
    28681 cctgaaagat ttggatgtgt ttactccgcc aaccttcgat gatgaagaag tcgctgaaca
    28741 taccaacctc gaaacgcact ttatcgattc cagcggttta atctcctggg acctgttcaa
    28801 gcaggatgcc gattatccgt ttgtggactg gaatttctcc ggcaccacgg aagaagagtt
    28861 cgccacgctg atggctatct tcaaaaagga agataaagaa gtttatattg ccgattacga
    28921 gcatctgggt gtctacgctt gccgcattat cgtgcctggc atgtccgata tttatccggc
    28981 tgaagatctg tggctcgcga ataacagtat gggcagccat ttacgtgaaa cgattctttc
    29041 gctaccaggc agcgagtggg aaaaagaaga ttacctgaac ctcatcgagc aactggatga
    29101 agaaggtttt gatgacttta cccgcgtgcg tgagctgttg ggtctggcga ccgggtcgga
    29161 taacggttgg tacaccctgc gtatcggtga attaaaagcc atgctggcgc tggctggtgg
    29221 cgatctggaa caggctctgg tctggaccga atggacgatg gagtttaact catcagtatt
    29281 tagtccggaa cgcgccaact attatcgctg cctgcaaacg ttgttattac tggcacagga
    29341 agaagatcgc cagccgctgc aatatctgaa tgcgtttgtt cgcatgtacg gcgcagatgc
    29401 cgtagaagcc gccagtgcgg caatgagcgg cgaagcggcg ttttacggcc tgcaaccggt
    29461 agatagcgat ctgcacgcgt ttgctgcaca tcagtcgttg ttgaaggcct acgaaaagct
    29521 gcagcgcgcc aaagcagcat tctgggcaaa ataaaatcaa atagcctacg caatgtaggc
    29581 ttaatgatta gtctgagtta tattacgggg cgttttttta atgccccgct ttacatatat
    29641 ttgcattaat a
//
LOCUS       DAZFXT010000055        29600 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.055, whole genome shotgun sequence.
ACCESSION   DAZFXT010000055 DAZFXT010000000
VERSION     DAZFXT010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29600
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.055"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            60..1349
                     /gene="uraA"
                     /locus_tag="ACHKW0_002563"
     CDS             60..1349
                     /gene="uraA"
                     /locus_tag="ACHKW0_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HFO7475380.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            1444..2145
                     /gene="hda"
                     /locus_tag="ACHKW0_002564"
     CDS             1444..2145
                     /gene="hda"
                     /locus_tag="ACHKW0_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HFO7475381.1"
                     /translation="MNTPAQLSLPLYLPDDETFASFWPGDNSSLLAALQNVLRQEHSG
                     YIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVPEVLDGMEHLSLVCID
                     NIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNLGLPDLASRLDWGQIY
                     KLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRTLFMTLDQLDRASITA
                     QRKLTIPFVKEILKL"
     gene            complement(2283..2642)
                     /gene="arsC"
                     /locus_tag="ACHKW0_002565"
     CDS             complement(2283..2642)
                     /gene="arsC"
                     /locus_tag="ACHKW0_002565"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HFO7475382.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(2663..4126)
                     /gene="bepA"
                     /locus_tag="ACHKW0_002566"
     CDS             complement(2663..4126)
                     /gene="bepA"
                     /locus_tag="ACHKW0_002566"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HFO7475383.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            4339..5400
                     /gene="yfgO"
                     /locus_tag="ACHKW0_002567"
     CDS             4339..5400
                     /gene="yfgO"
                     /locus_tag="ACHKW0_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HFO7475384.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(5438..6289)
                     /gene="focB"
                     /locus_tag="ACHKW0_002568"
     CDS             complement(5438..6289)
                     /gene="focB"
                     /locus_tag="ACHKW0_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocB"
                     /protein_id="HFO7475385.1"
                     /translation="MHEKQTLFDLQLSARKAAIAERIAAHKIARSKVSVFLMAMSAGV
                     FMAIGFTFYLSVIADTPSSQALTHLVGGLCFTLGFILLAVCGTNLFTSSVMTVMAKSR
                     GVISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHT
                     FTESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIP
                     FAIAIRHFAPTSFWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCYRA
                     IYLRQES"
     gene            complement(6307..8319)
                     /gene="hyfR"
                     /locus_tag="ACHKW0_002569"
     CDS             complement(6307..8319)
                     /gene="hyfR"
                     /locus_tag="ACHKW0_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HFO7475386.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPM
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHFDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRSMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGIGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(8349..8762)
                     /gene="hyfJ"
                     /locus_tag="ACHKW0_002570"
     CDS             complement(8349..8762)
                     /gene="hyfJ"
                     /locus_tag="ACHKW0_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HFO7475387.1"
                     /translation="MAEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(8755..9512)
                     /gene="hyfI"
                     /locus_tag="ACHKW0_002571"
                     /pseudo
     CDS             complement(8755..9512)
                     /gene="hyfI"
                     /locus_tag="ACHKW0_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
     gene            complement(9509..10047)
                     /gene="hyfH"
                     /locus_tag="ACHKW0_002572"
                     /pseudo
     CDS             complement(9509..10047)
                     /gene="hyfH"
                     /locus_tag="ACHKW0_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
     gene            complement(10057..11724)
                     /gene="hyfG"
                     /locus_tag="ACHKW0_002573"
     CDS             complement(10057..11724)
                     /gene="hyfG"
                     /locus_tag="ACHKW0_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HFO7475388.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYPFINEGNSDAQVIPVGPLHITSDELGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLIGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDLRFD
                     HPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFALDNMPDTPLLTEGFSY
                     KPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANWPVLRYMLRGNTVSDAP
                     LIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRNRSPLK"
     gene            complement(11714..13300)
                     /gene="hyfF"
                     /locus_tag="ACHKW0_002574"
     CDS             complement(11714..13300)
                     /gene="hyfF"
                     /locus_tag="ACHKW0_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HFO7475389.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLEAAWKYIIICTVGVAFGLFGTVLVYANVASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            complement(13305..13955)
                     /gene="hyfE"
                     /locus_tag="ACHKW0_002575"
     CDS             complement(13305..13955)
                     /gene="hyfE"
                     /locus_tag="ACHKW0_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HFO7475390.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(13967..15197)
                     /locus_tag="ACHKW0_002576"
                     /pseudo
     CDS             complement(13967..15197)
                     /locus_tag="ACHKW0_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(15214..16161)
                     /gene="hyfC"
                     /locus_tag="ACHKW0_002577"
     CDS             complement(15214..16161)
                     /gene="hyfC"
                     /locus_tag="ACHKW0_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HFO7475391.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(16172..18190)
                     /gene="hyfB"
                     /locus_tag="ACHKW0_002578"
     CDS             complement(16172..18190)
                     /gene="hyfB"
                     /locus_tag="ACHKW0_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HFO7475392.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFDFADFTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNLFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTQVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQSLRVVFSALFRLRQQFDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(18190..18807)
                     /gene="hyfA"
                     /locus_tag="ACHKW0_002579"
     CDS             complement(18190..18807)
                     /gene="hyfA"
                     /locus_tag="ACHKW0_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HFO7475393.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(19060..19530)
                     /gene="bcp"
                     /locus_tag="ACHKW0_002580"
     CDS             complement(19060..19530)
                     /gene="bcp"
                     /locus_tag="ACHKW0_002580"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HFO7475394.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(19530..20102)
                     /gene="gcvR"
                     /locus_tag="ACHKW0_002581"
     CDS             complement(19530..20102)
                     /gene="gcvR"
                     /locus_tag="ACHKW0_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HFO7475395.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            20248..21126
                     /gene="dapA"
                     /locus_tag="ACHKW0_002582"
     CDS             20248..21126
                     /gene="dapA"
                     /locus_tag="ACHKW0_002582"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HFO7475396.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            21143..22177
                     /gene="bamC"
                     /locus_tag="ACHKW0_002583"
     CDS             21143..22177
                     /gene="bamC"
                     /locus_tag="ACHKW0_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HFO7475397.1"
                     /translation="MAYSVQKSRLAKIAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            22390..23103
                     /gene="purC"
                     /locus_tag="ACHKW0_002584"
     CDS             22390..23103
                     /gene="purC"
                     /locus_tag="ACHKW0_002584"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HFO7475398.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            23258..23485
                     /gene="vapB"
                     /locus_tag="ACHKW0_002585"
     CDS             23258..23485
                     /gene="vapB"
                     /locus_tag="ACHKW0_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HFO7475399.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            23485..23883
                     /locus_tag="ACHKW0_002586"
     CDS             23485..23883
                     /locus_tag="ACHKW0_002586"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HFO7475400.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            24030..24893
                     /gene="ypfJ"
                     /locus_tag="ACHKW0_002587"
     CDS             24030..24893
                     /gene="ypfJ"
                     /locus_tag="ACHKW0_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HFO7475401.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLLVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            24908..26923
                     /gene="tmcA"
                     /locus_tag="ACHKW0_002588"
     CDS             24908..26923
                     /gene="tmcA"
                     /locus_tag="ACHKW0_002588"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HFO7475402.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            26997..27695
                     /gene="ypfH"
                     /locus_tag="ACHKW0_002589"
     CDS             26997..27695
                     /gene="ypfH"
                     /locus_tag="ACHKW0_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7475403.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(27805..28005)
                     /gene="ypfN"
                     /locus_tag="ACHKW0_002590"
     CDS             complement(27805..28005)
                     /gene="ypfN"
                     /locus_tag="ACHKW0_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HFO7475404.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(28033..29160)
                     /gene="dapE"
                     /locus_tag="ACHKW0_002591"
     CDS             complement(28033..29160)
                     /gene="dapE"
                     /locus_tag="ACHKW0_002591"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HFO7475405.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(29164..29520)
                     /gene="yffB"
                     /locus_tag="ACHKW0_002592"
     CDS             complement(29164..29520)
                     /gene="yffB"
                     /locus_tag="ACHKW0_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HFO7475406.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         7157 a         7711 c         7939 g         6793 t
ORIGIN      
        1 taagagtcgg cttttttttg agtaaagcgc ctataacaca taatacagag gataatacta
       61 tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact tttacagaca atcccgctta
      121 gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc ttatttcata
      181 ttaacccggc gactgtactg ttatttaacg gtattggaac gctgctgtat ctcttcatct
      241 gtaaagggaa aattccggct tatcttggtt ccagctttgc ctttatttca ccggtattgt
      301 tactgttgcc gttagggtat gaagtcgcgc tgggcggctt tattatgtgc ggcgtgctgt
      361 tctgcctggt ttcttttatc gtgaagaaag cggggaccgg ctggctggac gtgctgtttc
      421 cacctgcggc aatgggcgca atcgttgccg tcatcggtct ggagctggcg ggcgtagctg
      481 ccggtatggc gggtttactc ccggctgaag ggcaaacgcc agactccaaa accatcatca
      541 tctctattac caccctggcg gtcacggttt taggttccgt gctgtttcgt ggtttcctgg
      601 caattatccc gattttaatt ggcgtgctgg tgggctacgc gctctctttc gcaatgggaa
      661 ttgtcgatac cacgccgatt attaatgctc actggtttgc gctgccaacc ctctatacgc
      721 cgcgcttcga gtggtttgcc attctgacta ttctgccagc ggcgttagtg gttattgccg
      781 aacacgtagg gcacctggta gtaacggcta atatcgtcaa aaaagatctg ctgcgcgatc
      841 caggtctgca ccgttcgatg tttgctaacg gcctgtcgac cgtgatctcc ggcttctttg
      901 gctctacgcc aaataccact tacggagaaa acattggcgt gatggcgatc acccgtgttt
      961 acagtacctg ggttatcggt ggggcggcga ttttcgctat cctgctttcc tgcgtcggta
     1021 aactggctgc cgctatccag atgatcccat tgccggtgat gggcggcgtt tcgctgctgc
     1081 tttatggtgt catcggtgct tccggtattc gtgttttgat cgaatcgaaa gtggattaca
     1141 acaaagcaca gaacctgatc ctgacttccg tgattttgat catcggcgtc agtggcgcga
     1201 aggtaaacat cggcgcggcg gagttaaaag gtatggcgct ggcgaccatc gtcggtatcg
     1261 gcttaagttt gattttcaaa ctgatcagtg tgctgcgccc tgaagaagtg gtgctggacg
     1321 cagaagacgc ggatataaca gacaaataat gtaccgaccg ggcagtgttg ctgcccggtt
     1381 caaacatcat gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct ggttgaggta
     1441 tctctgaaca caccggcaca gctctctttg ccactttatc ttcctgacga cgaaaccttt
     1501 gcaagtttct ggccggggga taactcctct ttactggccg cgctgcaaaa cgtgctgcgt
     1561 caggaacata gcggttacat ctatctctgg gcacgcgaag gcgcggggcg cagccatctg
     1621 ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta tgtcccgctg
     1681 gataaacgca cctggtttgt tccggaagtg ctcgacggta tggagcattt gtcgctggtc
     1741 tgtatcgaca acattgagtg tattgcaggc gatgagttgt gggagatggc gattttcgat
     1801 ctctacaatc gaattctgga atcgggcaaa acacggttgt tgatcaccgg cgatcgtcca
     1861 ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc gactcgactg ggggcagatc
     1921 tacaaattgc agccactttc tgatgaagat aagttgcagg cgctacagtt acgcgcgcgt
     1981 ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg gctcgacaga
     2041 gaaatgcgca cgctatttat gacgttggat cagttggatc gtgcgtcgat taccgcgcaa
     2101 cgtaagctga ccattccgtt tgtgaaagaa attctgaagt tgtagataag gtgtttattg
     2161 tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt taatccatag tattcgtagg
     2221 ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg atggagcaca
     2281 tcttaaccca cgatttccag tacctgctcc ggtggacgac caatccgcgc tttgccattc
     2341 gcgaccacaa tcgggcgctc cattagcttc ggattatcca ccattgcctg aatcagcgcc
     2401 tcttcgctaa gcgaactgtc agccaggttc agttctttat aaagatcctc tttctggcgc
     2461 atcaattcac gggcgctatt catccccagc attttcagca aatcgcgcag cgttgccgca
     2521 tcggcgggtg tctcaaggta gagtaccact tctggctcca cgccgttttc tttcagcagg
     2581 ttcagcgttt cccggctctt tgaacagcgt gggttatggt agatttttac ctgtttggtc
     2641 attgttcttc ctttaatgcg aattacatct tggtataagg cttaaagcgt tcctgcagct
     2701 ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct aatttcacct
     2761 gcgaactggc gctactcaac agcgaaatgg cctgatcgag tcgtccggcg agcgcataac
     2821 cttctgctcg cgcagccagc tcctgatcgc ggttatttag cgcggcttcc gcctgtgcca
     2881 gtaaatccca gccgttgctg tcatctttat tattaaaggt gtagcgattc agaatattgg
     2941 ccgcttcttg tggttgaccg ccttgcagat aagcgttcgc caggttgagc tgcaacaccg
     3001 gattggtgcg taaatcgcgg gcatttttca gacgattgat cgcctcattg gctttgtttt
     3061 gcccaagatc gatatcagta gccagatcga gataccatgc gttgccaggt tctgccgcca
     3121 gtaacggttg cagcgtttta cgcgcctcgt cgtatttatt ggcttccatc gcctgtaaag
     3181 cacgaccata ttgtgctgct cgttgctgac gaacgtttcc tttcgcccat tcatccagca
     3241 aatcactggt gagctggtta cgtccggaat tatacatccc cagtgtgcgc gctttcgcca
     3301 gatagaaatc ttccgacgac tgcaccacca tcgggcgcat ctgattagca cggttgcggg
     3361 catctgccag acgactttcc ggcaacgggt gagtcaataa aatttccggc ggacgcgagg
     3421 agtaacgcgc ctgatcgagt aatttttcga ggaaggttgg catcgcctgc ggatcgaatc
     3481 ccgagcgttg cagcacctga ataccaatgc ggtccgcttc ctgttcattt tgctgggtga
     3541 aactgatcat cccctgacgc gttcccgcca gtgtaccggt cagcgccgcc atccccgcct
     3601 gcggactggc catcgccagt aaaatagaac ctaacgcgcc gacccaggtc agcggcgcgc
     3661 tgcgctgctg atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg gagatttcgt
     3721 gcgccataac tgaagccagt tgactttcgt tatcggaata acggaacagg gcagagtgca
     3781 gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg atcagaaaga
     3841 aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag acgcatcccc agcgaattaa
     3901 tatattgcgt taacagcggg tcattaatta acggcgcgct gccgcgtagc tggcggacat
     3961 aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg gaggttccca
     4021 tatccggcaa ggtgtctgcg ctgtcggcaa acgccggggc tacctgacca atggtcatag
     4081 cagcaatgag ggttgcaacc aggttttttt tcaactgcct gaacataacc tctatcctgt
     4141 atttctgaag atagtcattt gaccgatact gttaaatatc gttcccaccg cgccgccttt
     4201 gagtgtaacg gcgaagatta gcccactcaa atgacttttc agtttcctga atccgtcata
     4261 aaaagcgaag tcttttttgt gacatttcga tacaattgcg gatcgcaatc ccgctcttga
     4321 ggttcaggga aggtctttat gctcgaaatg ttgatgcaat ggtatcgccg ccgttttagc
     4381 gacccggaag cgattgcctt gctggttatt ttagttgccg gatttggcat tatctttttc
     4441 tttagtggcc tgcttgctcc gttgctggtg gctattgtgc tggcctattt gctggaatgg
     4501 ccaactgtgc gcctgcaatc tattggctgc tcccgccgct gggcgacgtc gattgtattg
     4561 gtggtttttg tcggtatatt gctactgatg gcgttcgtgg tactgcctat cgcctggcaa
     4621 cagggcatct acttaattcg cgatatgccg gggatgctca ataagctttc tgactttgcc
     4681 gccacgttgc cgcgccgcta tccggcgtta atggacgcgg gcattattga tgcaatggcc
     4741 gaaaatatgc gcagtcggat gctgaccatg ggcgattcgg tggtgaaaat ttccctcgcc
     4801 tcgctggtcg gtttgctgac cattgccgtc tatctggtgc tggtgccatt gatggtcttc
     4861 ttcctgctga aagacaaaga gcagatgctg aacgccgttc gtcgggtgct gccgcgcaac
     4921 cgtggactgg caggacaggt gtggaaggag atgaatcaac aaatcaccaa ctatatccgc
     4981 ggcaaagtgc tggagatgat cgtggtgggg atcgccacct ggctggggtt cttgctcttt
     5041 gggctgaact attcgctgct gctggcggtg ctggtcggct tctcggttct tattccgtac
     5101 attggcgcat ttgtggtgac cattccggtg gttggcgtgg cgctattcca gtttggcgca
     5161 ggcacggaat tctggagctg cttcgcggtg tatctgatta ttcaggcgct ggacggcaac
     5221 ctgctagtac cggtgttgtt ctccgaagcg gttaacctgc atccgctggt gattatttta
     5281 tcggtggtga tcttcggtgg tttgtgggga ttctggggcg tattcttcgc cattccattg
     5341 gcgacgctga tcaaagccgt gatccacgcc tggcccgatg ggcaaatcgc gcaagaataa
     5401 tcttcagccc gacgaactgt ttcgccgggc tgatttttca ggattcctga cgtaaataaa
     5461 tagcccgata acacatactc accagcactg caccgccgat aatattaccc agcatcaccg
     5521 ggagcagatt ggcggtaata aaatggctga ccgtcagtgc cggaaaattg tctgcgctac
     5581 tgtgcgccag ttgccagaag gaggtagggg cgaaatggcg aatggcaatg gcgaacggaa
     5641 tcacaaacaa attggcgata cagtgctcaa agccactggc gacaaacagg gtgatgggca
     5701 aaatcatggc gacgattttg tcgcataacg aacgcccgca ataactcatc cacagcgcca
     5761 ggcaaaccat cagattgcac ataatgccga ggctgacaga ttcagtaaat gtatgatgca
     5821 ttttgccctc ggcgcagtgt aaaaccgcga ctccccacat cgcgttttca ctcatcacca
     5881 gcccggaaaa ccagattaac aaactgaaac aggcaatacc tgccagatta ccgcaggcca
     5941 ccagaagtgc gttaatcagc caagttcgcc aactaataac gccccgactt tttgccatta
     6001 ccgtcattac cgacgaggtg aacaggttgg tgccgcaaac cgccagcaaa ataaagccaa
     6061 gtgtaaagca aaggccgccc accagatggg ttaatgcctg tgaagacggg gtatcggcga
     6121 taacggaaag gtaaaaagta aatccgatcg ccataaacac gccagcggac atcgccatta
     6181 aaaagaccga cactttactg cgggcaattt tatgggcggc aatccgttca gcgattgccg
     6241 cttttctggc gctcaactgc aagtcgaaga gagtttgttt ctcatgcata ctcctgcaaa
     6301 agcagattac aacacctcgc gaaccgagat tcccagacgc tgcattcgtg acagcagcgt
     6361 ggtgcgcttc atccctaatc gtgtcgccgc gccacggggc ccggcaacaa tgccattggt
     6421 ttctcgcaat acctgaacaa tgcgctgacg ttcttcttcg tcattctctg gcgtcgtcgg
     6481 gtgcagcaac tgcgccattg aactgcgaag cgctgaatct tcatttagcg tcggcagtaa
     6541 acggctttgt cggacattaa gatgtaaatt cagactgtta ccgcgagtca gcagtaccgc
     6601 tcgctcaatc acgttttcca gctcgcgcac gttgcccggc cagtcccacg acatcaactg
     6661 gcgtagcgcc tcggtcggga tggcgtcaat tgaacgattc atatggcgcg ccattttttg
     6721 cgtgaaatgc tttgctaaaa gagggatatc ttccggacgg tcgcgcagcg gcggcaattc
     6781 cagtgggaag acattcaggc gataaaagag atcgctgcga aactggcgat cttcaaccat
     6841 ttgccacaaa tcacggttgg tggcggcaat gactctgaca tttaccggga tcgttctact
     6901 cccgccgaga cgctcaatct cccgttcctg caatacgcgc agcagtttag gctgaagttc
     6961 taacggcaga tcgccaattt catcgagaaa caacgtgccg ccatcggcaa tttcaaaacg
     7021 accacgatgg gtattaatcg caccagtaaa cgcccctttg tcatgaccga ataactcact
     7081 ttccagaaga ctggcgggga tggcagcgca gttgattttg accagcggct tgtcgcgtcg
     7141 cgggctaagt tgatggatcg ctctggcgat cacctctttg ccggttccgg tttcaccgca
     7201 aatcaacacc gtactgtcgc tcttcgccac aatatctacc tgctggagta ggtcttccat
     7261 tgcctggctt tgatagataa tgtcaccgat gcccagattc gaaaggagct gctcgctaag
     7321 ctggtggttt tcttgctgca aactttcctg caaatcggtc atgctacgcc aggcatcggc
     7381 attgccaacg gcaatagcga tgcgatcggc tatgtgttgt agtagctggc agttttcctc
     7441 actaaagaga gtggaagagg tatgcgccag caacaatgca cccggtgtat ggttgccaaa
     7501 ggtaagcggt ataaggagcg cagattcgca gccgttagat gcaagtaaga gtaataacgg
     7561 gtcgcgttgc cagagaaaca gatcgtctgc acggtgcgtc aaggtcggtt gtcgggtttg
     7621 tagcgtttgt gtcagcaata cactgtcgcc aggcatactg cgtggcagac acgcacaatg
     7681 tgaggcagaa agatcgctgc accacaggct aaacttctcg ttctttcgat gatcgcccag
     7741 taccatactg actgaagcca gaccgaaaaa atgatggatc tcacgagcga cgtcagcgat
     7801 cagatcgtcg agatcaaaat gtgacagcac tgcattagta atatccacca gtacccgatg
     7861 atgatcgcga tctttctcca gcaaacgcag cgccacctgc tgttcttcat cattgagcca
     7921 tgattgcaga accggggtga ttatccgcgc cagccagtta aacagtgtgc aatcctgggg
     7981 agtaaaacgg ctgccgtggg tacgaataaa gcgtagctcc gccattccac tactgccgag
     8041 aaggacggcg tgacgcgaat aatcgaccat ctcgccctga tactctgttg ttgccgtcac
     8101 atcgctgcca tcatcatggt agcggtaata gaagttgctt ttgagaacat gcagttcaat
     8161 aagttgtacc gggctgaacg gcggcggcat cgggatgaag gcgcgtaata aagacgcctt
     8221 accgtgtttc tgtgctaacc gctccgcgag cattccgttt accgcttcaa ttgttattcc
     8281 ttgtggcggg gcaaacatcg cctcgtctga catagccatc aacagtcctt attaatgaga
     8341 aatccttctc acgcaatttt tctggccatc agatagatgg ccggttcctg cacaatttca
     8401 tccaacagat gaatgaattg aatgctacgc gcctgccaca cagacgtcgc gtcatctgcc
     8461 agtggcgcga atgcccgggt caacagtgcg gtgtggctgg cgtcaataac aatctcacca
     8521 aaagtcatca cccccagcat cttacgtcgg gcttgctcct cttcgaccag tgcaagccaa
     8581 tcttcgtatt ccgtcagtgg gcagcggaag gcgacattca gacaatcaat cacgccaacg
     8641 tgatgaccga tagccagcga gtaatacatt acctgggagc tgtgttccgg catctcgtcg
     8701 ctactggcga caaacttttt tcgcagcgtc cagaaaacaa tttccccgca ctcctcagcc
     8761 atgtaacccc cggagaacgc gaaagagttg ctgaacgata ctattgagac gcggatcgtc
     8821 agcttcgcgc aaccaggtgt taacccgatt tcctgtagga tcgtcgctta aatggcgcag
     8881 gagccgatcg caaatttctc gcccctgacg atagcccgcc agctgccgcg cttctcgctc
     8941 aatggcgata cgctgtgatg gcgggatctg cggccacagc ggttgcatgg tcaccccggt
     9001 gggatcgcga taatccacag cgtgaatctt ctgttgcagc aaaccgagcg ccacggcgaa
     9061 accgtgaatg gtggcggccg gtgttggcgg gcagccgggg atccaaacat caatggggac
     9121 aatggtgtcg ctgccgcccc agacgctgta gagatcgtgg aaaatccgcc gccggcaccg
     9181 cacgcgccgt aggaaacaca gattttatga tcgggggcag actcatacgc ccgaagtgca
     9241 ggcatacgca tcgcccgggt gagtgcgcca gtaaataaca aaatatcggc gtgacgcggt
     9301 gatgaaacaa ccttaatgcc aaaacgttct gcgtcgaata ctggtgtaat ggcggcaaaa
     9361 atttcgattt cacaggcgtt gcagccaccg caatcgacgc gataaacgta ggctgagcga
     9421 cggatatcct gtagcaaatg ccgcttcatc ttttgcgttt gctcgtccag cgtgatgggc
     9481 tggctgacat attgcgtaag cactggactc atagctgctc cttagccacc agcggtacat
     9541 ctgtatcgtc gttgagcagc gtcgcgcgtt gtttgcactc cgggcagacg ctcgcttgcg
     9601 cccataacat ttcgcggttt tgtggcgcat tttgttgctg tgccaacaat tcagcagcca
     9661 gtgcgacggt tttttgctgg gcaaacgggc gttcgcaacg gctgcaacgt tgtagatgga
     9721 acgtcgcgcg ggtatagaga tcggctttat tggtgacggt cagttcaaag ttattggtaa
     9781 gctgatggct ctggtcgggc acacttcttc acaacgtccg cagtaaatac aacgcctcag
     9841 atagagctgc caggtgcgcg aattttgctg gtcgtcggtc tggatagtca gcgcatttgc
     9901 cggacaagca caggcgccgc aggcaataca ttggctgggc atcaggtccg gtttcccgcg
     9961 aaagcctggg ctgacctcca gtggcgcgaa gggatatttc accgtcgcgg ttccggcgcg
    10021 cataatagtt ttcagtaact tcagcatctt ctgtccttac ttcagcggcg aacggttacg
    10081 atcaatgccg tagcgttcga tctctttata cggcacggtt tttgactggc gcttgcgcac
    10141 atctaccagc gtcacacggt cggtacagga gtagcaggga tcaaggctac cgataatcag
    10201 cggtgcgtca gaaacggtat tgccgcgcag catgtaacgc aacaccggcc agttggcgta
    10261 ggtggcggca cggcagcgcc agcggaacaa tttttggtta tcaccgagca tgctccagtg
    10321 cacgtcttca ccgcgtggcg cttcaacaaa gcccagcgcg aatgcgtgag gtttatagct
    10381 aaagccttcg gtcagcagtg gggtatccgg catgttgtcg agagcaaatt ctagcattgc
    10441 cagcgaatca aacgtctctt tgacacggac catcacgcgg gagaaaacat cgccgccggt
    10501 aaaggtaaac agtgtttttg gaatattgcc gtagtcggcg taggggtgat caaagcgcaa
    10561 atcacgggcg atttgtcggt cgagaatgcc aatgccctga gtgcgttgtt ccatattcgg
    10621 cgtagcaagc agcatctcta ccagctccga cacgtcggcg cgcatctcgc gcaccagttt
    10681 cagcgtttgc agacgttgct ctttgagaat atcgcggcga acaccaccaa tcagattcag
    10741 accgtaggtt ttacgcgacc cgatcagcaa ttccgccatc gtcatcgact tttcccgcac
    10801 gcggaaaaat tgcataaagc cggtatcaaa accaacgaaa tggcaggaga ggccaaggtt
    10861 aagcaaatga ctgtgtagcc gttcgacttc cagcagaatc gagcgaatag tatgtgctcg
    10921 ttgcggcacc tcaatcccca gtgcattttc aaccgagttg gtataggcca cactgtgggc
    10981 aaaaccgcaa atcccacaca cgcggtccga taagaaggtc acttcgttgt agcccatccg
    11041 cgtttctgcc agtttctcca tgccgcgatg gacataaaac aggcggtaat cagcatcgac
    11101 aatttgctcg ccatccacaa acaagcggaa gtgacccagt tcatcggagg tgatatgcag
    11161 cgggccgaca gggatcacct gcgcatcgct gttgccctca ttgataaacg gatacgtttc
    11221 ggaatcagtc gtcggttcag ggcgcagtcg ataatccatc gcatctttgc gcagcggatg
    11281 catatcttcc ggccagtcat cgggcaacac caggcgacgc tgatccggca ggccaaccgg
    11341 aatcagcccg tacatatcgc gaatttctcg ctcgccccag accgcggcag ggacgcgcgg
    11401 tgtgactgac ggaaactccc gactgtcggc atcgaccagt gccttcacca cgatccagca
    11461 tttttcggcc ccttccattg aaagggcata ataaaccgcg taatgaccgt taagtgtccg
    11521 ctcgtcgttg ccaaacagga ctggaagcca gccatcatgt tgataataaa gataatgtac
    11581 aacgtcaggc agcagattga ttttcaccgt aatggtgacc tgttcaggcg tttgtcgctc
    11641 ttcatccagc accgcgccgg ggaactgcgt tttcagggcg gcgagaatcg cttcgccacg
    11701 atttgacgat gaattaacgt tcactgtgtt tctccgaaac agatgcggtg cctgaaggca
    11761 aaaaatcatg ccaggtgcta cgttgtgcag gcaggtcgtg cgtccctgag aggactatag
    11821 tggaagcgcc cgccaggatc ctgatgacag gttgtggaat atgcgttccc atcgccagca
    11881 tcatgaccag cagaatcacc attggcgagg tggtcaacca gccgagatca ccccggttaa
    11941 cggcctgcgg cggtttcgcc attaacaccc gcgctgccat ccgtaccagg cccgccagca
    12001 ccagcgttaa cagcaataac agcaggacga taatcagcag gtgattacgt gccagtccgg
    12061 cggtaatggt cataaattcg ctaagaaaaa tgttgaaggg cggcatccct gccagcgcca
    12121 gcgccagcgc accgccgcca aacagcacgg cggtaaatgg catgattttg agcatcccac
    12181 agacgacgtt gagatcgcgc gtgccgtact tgagcagtac attgccggaa ccgcagaaca
    12241 gcagcgtttt tgccagactg tggtttaagg tgtgcagcag cgcggcaaaa attcccagtg
    12301 gcccgccaat gcctagcgcc accgcgacca gtcccatgtt ctccacgctg gagtacgcca
    12361 gcagacgctt aatgtcccgc tgtaccagaa tgaaaaatgc cgccacggca accgacaaca
    12421 tgccgaagat gagcaacaac cggttgggga aatcgctgcc gatggcctgg cagataatga
    12481 tgtaatagcg aattagcacc aacagcgcgc agttcagcaa tacggcggag agcagggcgc
    12541 tgaccggact cggtgcttca ctgtgagcat ccggcagcca ggcgtgcatg ggaaatagcc
    12601 cggttttggt gccaaagcca attagcacaa acacaaaggc caacagcatt aatgttgggt
    12661 caagcaagga cgattgctta agaacctcgc tccagaatat cgccatttct gcctgcggca
    12721 taacgctggc gacgttggcg tataccagca cggtaccgaa cagaccaaaa gcgacaccaa
    12781 cagtacaaat aatgatgtac ttccatgctg cttccagcga tgaacgctga ccgtaaatgc
    12841 ctaccagaaa cgccgagctt aaggtggtgg cttcgatcgc cgcccacatc acaatcaggt
    12901 tattgctggt aacaaccagc agcatggtaa acaaaaacag atggaagaaa ccgtagtaat
    12961 cgcacagcgt aacgggggaa agctcgccgt gtgccacttc atgacgcatg tagccaatcg
    13021 agtaaacacc ggtgagaaag ccaatcacac caagaatggc gaggaacaaa ccgcccagac
    13081 catcaatatg aagccacagt cccgcagcga atatttctcc tgcatcagcg gcagtttgga
    13141 ccacccagag tgccagaatc agcagcagtg tgatccctaa gctatgtaat acagtcaccg
    13201 tacaagtcgc agagagcccc cgtttccggc aggcaaaaca gagcagcgaa aaaagcagcg
    13261 gcgtgagcag gagtaaagcg aacatcacag aataactcat ctcattatcc cttcagcgcg
    13321 gtcaagttgt tcacgtccag cgtgccgtgg gtacgccata tttttcttgc cagtaacacc
    13381 atcacaatga cggcgaagat ggcgtcggta gcgataccta tttccaccag ttccggtgct
    13441 cgccaggcaa gaagcgccag caccagatgg gagccgtttt ccatcaggca gtaaccaaaa
    13501 atttgccgca ggatattgcg ctggctgaca atgcacagca ggccaagcag aaaatgacct
    13561 aacgctaccg ccagcgccgg tttcagcccg gtagccatcg gtagcttcac gggctgaacg
    13621 acaaatgcgc aaagcaggac aattaacgcc gcgagcagtg ccatcattgc cggaccgaat
    13681 aacgcttttt ccgggatgtt ctggggaata tttcgtgcag cgtaagtcat gattaacggt
    13741 accagcagca ctttggtgat aaaggcgctg gcggaccaga tcagcagttg ctctgcggcg
    13801 aacaggcacg agagagtggc gaaaatagac accagcacca gcgactggca ggcgtaaaat
    13861 ccgcatgaca ggcgatagct tttgacgcta atcacaaaca gcgatgtcag catcatcagt
    13921 cccgccagat tatttacgat catagaaccg gtcattcatc atctccttac tgcaaccagg
    13981 tcgcggcgat tacgctggaa atcagcaaca tcacaatcag tacaatcaac accaggcgca
    14041 ttgaccctgg cagcggtgcg gcatcggcga cggcctcgct cggtttgcca ggcacaacgc
    14101 gaccaaacca gcgaataaac caggcgaaac tggcgaccga ttcaatcatc agcagaatca
    14161 tggcgggcag caggatccag tactccactg acaacgcaaa accggcagca aacagcggga
    14221 atttactgaa gaagccgttg aacggcggca cgccggtaat cgccagcgct gccacgcaga
    14281 aacccacgcc tggcaacggc agggtgtgca atacgccacg cagacgcggc aacaagcgcg
    14341 tgccgcagct gtaactcagc gcacctgcta caaggaaaaa caggctttta gcgaacgcgt
    14401 ggttgacgat gtaggcgatg ctgccctcca gtgccagccg cccactttca ccatcgatgc
    14461 ggcgtggaga taggcgctga tcggtgttgg cgcttccatt gcatccggta gccacgcttg
    14521 catcggtagc tgggccgatt tcccccacgc ggcaaacagg atgccgccat aaaccagata
    14581 acgtgcgtcg ccatgtaact cgctcatcgc gctaagcaca aacgttccgg tctgcaaaaa
    14641 cagcgtggcg gcggcaagat acaaccccaa cgaaccgata tgggtgataa gcaacgcttt
    14701 tagtgctgaa cgctgcgctt tatcgctctg gtaataactg atcaacgccc aggagcagcc
    14761 gcccgtaatt tcaaaaaaca acaactgacc gagcagcgtc gaggagagta ccagtcccgc
    14821 catcgcgccg ataaacacca gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg
    14881 ttcgcgattt ttatccgtca gataacccgt ggagtagatc gtgaccagca aaccgagaaa
    14941 caccaccaca aacagaatca gcgtactcac gcgatcaatg accaggccaa acaatgccac
    15001 atcccccacg ttgaccaacg tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga
    15061 gatcagcgac aacatgcaca gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc
    15121 cgacgttgtg gcgaaaacga aacgaccagt gcgccgataa aaggcagcat taacgtcgtc
    15181 agagcaagat tttccatatt ccgtcagtgc tccttacaga ccggttaacc agaagaccca
    15241 tgcaagcgca gcaaggctga agccaagcca ggtcacatgg tgaatgagta aaaaacgccc
    15301 gcgtgccagc gtgttttctg cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag
    15361 cgtaacgaca agtgaagtca gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg
    15421 cagaaacagg gccacaaaca gtgctgccat cacgacctgt ttcagcccca gcccccattt
    15481 cgctagcgcc agcccggcac cggaatattc ggtcagcggg ccttcctgta attcctgttc
    15541 tgcttcagca acatcaaagg gaatttttcc catctcaatg aagcaggcaa aaccacaggc
    15601 cagtaacgcc agtacggtgg ttagcggcga gttccagccc gttgccagcg tattgctgat
    15661 catctcgata tgcgtggaac ctgctatcag cgccaatacc agcagtgaga gaatgagcat
    15721 gggttcgacc agaatgccga gcgtcaactc gcgactggca ccgactcccg caaacgggct
    15781 tccggtatcc agcccggaaa gagcaaagaa aaaacgaaac agggcaagaa gatagataag
    15841 ggtgatcaga tcgccgccgc ccgcaaaagg ggaaacggta ataaaaagtg gtagggccat
    15901 cgccagcacc agcatgctgc tgattaatac ccacggcatc aggcggaaca tcagacctga
    15961 agatataggc gcaacttcct ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca
    16021 gatccccggc ccgcggcggg agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa
    16081 aagcggggtt agcatcagca gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc
    16141 gtcgtaaaga gtttgtctca tggtgatttc cttagacggc aatagcgatt agcagcacaa
    16201 ccagtgcggc gaccacatac aggcaataga gacgaaagtc gccgctttgc agatgctgta
    16261 tttctttggc cagccgttgg gtggcgctca cgatggggcg gatcacccgc tcatcccaga
    16321 agggttctgt gctctgagcc ctggcggtga cgtgcgcaag acctttattt agcctcagcg
    16381 tagggtcgaa ctgttgtcgt agacgaaata gcgcagaaaa gaccacacgc agcgactgca
    16441 tcacgccatt gcctgacggg gccatcgcat tttcccagcc gtagccgcat gcccaggcat
    16501 ctcctgtgcg acgaaacgct gcgcggcgcg aacgacacca cagccagtaa aggccaggca
    16561 gcaaaggcat cgccagtagt aacagcaaca gtaatgaggg ggtgacctgt gtatgaaacg
    16621 tgccgggtac aagtgctatt ccgctggcga cagtgacggg aggggtattg gtaaacgcat
    16681 gggcaatatg cattatcttc ggtgccagcc aactggcact aagcgcaatt aatacgcaga
    16741 gtgccgcgag cagtagcatg gcgacgatca tcgtatttgg cacttcctgt gcctcttcag
    16801 cgtgtgtact gcgcggcgca ccacagaaag taataccgta catttttacg aagcacatta
    16861 ctgccagccc accagtgact gccagcatta ccatagcaat aggacccgca agttgtagcg
    16921 ctacggcttc cacacgactt agtgagaaca gcgactgcca ggtgtaccat tcgctgataa
    16981 aaccattcag cggaggaatg gctgatatcg cgaggcaacc aatcaggcat gctgcggctg
    17041 tccacggcat ccgtttcgcc agtgccccca ttttttccat gtcgtgggta tgcaaacgcg
    17101 aaataatcgc gcccgcgccg agaaatagca gccctttgaa cagcgcatgg ttgagcagat
    17161 gaaacagtgc gccgagcagt ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga
    17221 ccatcgccac accgactgcc agcaaaataa tgccgacgtt ttcgacggta ctccaggcca
    17281 gcagccgttt gatatcctgt tccgccagcg catacagcac gcccaggagc gcggagattg
    17341 cgccgatcgc catcaccaga atgccccacc acagcggcaa acccgtttgc gccagcaggt
    17401 ccatcgctac tttcaggatg ccgaaaatac cgattttgac cattacgcca gacatcaacg
    17461 ccgaagcgtg cgatggtgcg gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca
    17521 tcccggcttt cgcgccaaaa ccgaaaaagg ccagcaggaa caccgccgac gccagccccg
    17581 gagaaagtga aagcgtgcgg aaactggcaa aatcgaggct gccgctttcg cgccccatca
    17641 gcaagaaggc gatcataatc agcaccgatc cggcgtgggc gataaaaaag tagagcatgc
    17701 cagcgttgat cgacgtttta tcctgcctgg caatgaccag aaaccaggaa gacagcgaca
    17761 tcatttcaaa cagcacgatg aaccaaaaag cgttgtccat caccagcagg gcaaccatcg
    17821 atgcgatgaa aagattcata aagaagccca tcgccgccgc gcctttgccc tcgtattcgc
    17881 gcatataagt caatgaatag agcgaacaaa ccaccaccag caaggagata accagcacca
    17941 taaatgccga gaggctatcc attcgcaggg taaaatcggc aaagtcaaaa ggggtggcaa
    18001 aacgggcgac taaagttaca ccagcatgta attgcgtaat tccgctgatg atgccaatca
    18061 cgccgcccac cagcgatgtg atgccggaaa gcttaatagc cagtctgtcc agaccgagta
    18121 aaaacagcga agccagactg gcaaacagat agaggatcag cgaccaggtt aataattgca
    18181 gggcatccat tagcgttgct cctgagtgag ggaaagggga tcctccccgc cattggcaaa
    18241 ccagcttgcg gcaaggcgct gtttttcttt catctgtcgt tgcaggctat cgtcggtgat
    18301 cagccgtaac gcctgattcg ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc
    18361 acacaggtcg catttcaccg cgacggtctg gacacccggt tcccagcgca gcaatgcatg
    18421 ttggggcggc acgttttctt cgccgtcttt gagtgagcct tcagcctgaa aaacatattg
    18481 cgcaggggca ttcaccggac ggcttcctga agcgctgatt gcgccaaatg ggcagaccac
    18541 ggcgcaaagc ttgcagccaa tacagaggct ttcgttgagt tggatcgcat catccctctg
    18601 ggagatggca ttaaccgggc agacctgcag gcaaggggct tcctcacagt gatgacacac
    18661 gacaggggca gtgagtgttg acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc
    18721 ttgcgtttta tgcacgtccg aacaggcagc gagacaggta ttacatcctg tacaccacag
    18781 tggttcggcc accacaaagc ggttcatgtc ggctccttga aaatgatatg aaacattgca
    18841 tttcatattg cataaaggat gccattctga ttaactcaat atattcaata agttatgtat
    18901 tgactggatt ctgaacggta caaaaatgat ggtcattaac gttcagcaaa aaatggattc
    18961 gacacccgtg tcgcagggga aagcgagaat gtgatcttga tcacgagaag tgaggtatgc
    19021 tggccgcaag cgcagccagc acggaatgga gcaaagtaat caggcgtgtt ctttcagcca
    19081 gttcagcaca acgtcgtggt gattgctggt tttgaaatcg tcaaagacat gttcgatttt
    19141 gccatcagcg tcaatcagga agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa
    19201 ggacttttca ccccagacgc cgaattgttc gcacacctgg tggtcctcat cagataggag
    19261 cgtaaagtta agcagctctt tttccgcaaa acgggagagt ttttcgggtt tatcggtgct
    19321 gatacccagc acatcaacgc ccgctttttt caactcatcc atgttatcgc gtaagccgca
    19381 ggcctgtacg gtacagccgg gggtcatggc tttcgggtag aaataaacca gaacacgctg
    19441 tccctggaag tcggtcaaat taacttgttc tccgtcttga tccggcaagc taaatttcgg
    19501 tgcgatatca ccggctttca gtggattcat tacttaactc catcctgttc atcatgttgg
    19561 gaataattga cgacgttaat actgccttgt gcattgagtt ctgtacatag ggctttgaac
    19621 gcttgctcaa tatttgctgc gtccgcagat gcggggctgt gggcggttat ctgaatatgc
    19681 aactgcgcag cccgttcatt ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc
    19741 atatgatgcg cgtcgaaaag tgctgtgaag cgttcaatta aatgcgggga gtctgccaca
    19801 tcgacctgaa cccagacaga tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc
    19861 atcacgatta aaagatccag ttcggcacct ttcaacggta acgttgattc aatcagagta
    19921 atggcattcc atgaaccgga aagcagcata ataaacgtga actcttctcc cagcatcgcc
    19981 aggcgactgt cttcaatatt acagccgcaa ctactgacat gacgggtgat ggtgttcaca
    20041 attccagggc gatcggcacc caacgcagtg atcaccagat aatgttgcga tgacagtgtc
    20101 aaactggtta ttcctttaag gggtgagttg ttcttaagga aagcataaaa aaaacatgca
    20161 tacaacaatc agaacggttc tgtctgcttg cttttaatgc cataccaaac gtaccattga
    20221 gacacttgtt tgcacagagg atggcccatg ttcacgggaa gtattgtcgc gattgttact
    20281 ccgatggatg aaaaaggtaa tgtctgtcgg gctagcttga aaaaactgat tgattatcat
    20341 gtcgccagcg gtacttcggc gatcgtttct gttggcacca ctggcgagtc cgctacctta
    20401 aatcatgacg aacatgctga tgtggtgatg atgacgctgg agctggctga tgggcgcatt
    20461 ccggtaattg ccgggaccgg cgctaacgct actgcggaag ccattagcct gacgcagcgc
    20521 ttcaatgaca gtggtatcgt cggctgcctg acggtaaccc cttattacaa tcgtccgtcg
    20581 caagaaggtt tgtatcagca tttcaaagcc atcgctgagc atactgacct gccgcaaatt
    20641 ctgtataatg tgccgtcccg tactggctgc gatctgctcc cggaaacggt gggccgtctg
    20701 gcgaaagtaa aaaatattat cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac
    20761 cagatcaaag agctggtttc agatgatttt gttctgctga gcggcgatga tgcgagcgcg
    20821 ctggacttca tgcaattggg cggtcatggg gttatttccg ttacggctaa cgtcgcagcg
    20881 cgtgatatgg cccagatgtg caaactggca gcagaagggc attttgccga ggcacgcgtt
    20941 attaatcagc gtctgatgcc attacacaac aaactatttg tcgaacccaa tccaatcccg
    21001 gtgaaatggg catgtaagga actgggtctt gtggcgaccg atacgctgcg cctgccaatg
    21061 acaccaatca ccgacagtgg tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg
    21121 ctgtaaagtt tagggagatt tgatggctta ctctgttcaa aagtcgcgcc tggcaaagat
    21181 tgcgggtgtt tcgcttgttt tattactcgc tgcctgtagt tctgactcac gctataagcg
    21241 tcaggtcagt ggtgatgaag cctacctgga agcggcaccg cttgcggagc ttcatgcccc
    21301 ggctggaatg attttgccgg tgacctccgg tgattatgca atcccggtga ccaacggtag
    21361 tggtgctgtc ggtaaggcgc tggacattcg tccaccagcc cagccgctgg cactggtttc
    21421 tggcgcgcgt acccagttca cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg
    21481 caatactctg tggccgcagg tggttagcgt gctgcaggcg aaaaactaca ccatcaccca
    21541 acgtgatgat gctggtcaga cactgaccac cgattgggta caatggaacc gtctggacga
    21601 agacgagcag tatcgtggtc gttatcaaat ctctgttaag ccgcagggtt atcagcaggc
    21661 ggttacggtt aaactgctga acctggaaca ggcgggcaaa ccggttgcag acgcggcttc
    21721 catgcagcgt tacagcacgg agatgatgaa cgttatttcc gccggtctgg ataaatctgc
    21781 cactgacgcc gcgaacgctg cgcaaaatcg tgcctccacc actatggacg tacaaagtgc
    21841 agctgatgac accggtttac caatgctggt cgtacgcggg ccgttcaatg tggtttggca
    21901 acgtctgcca gcggcgctgg aaaaagtggg catgaaagtg accgacagca cccgttcgca
    21961 gggcaacatg gccgtaactt ataagccgct gtctgacagc gactggcagg aactgggcgc
    22021 gagcgatcca ggcctggcat ccggtgacta taaactgcag gttggcgatt tagataaccg
    22081 cagcagccta cagttcatcg atccgaaagg tcatactctg actcagagtc agaacgacgc
    22141 gctggtagct gtcttccagg ctgcgtttag caagtaaaaa tacagggctg gaatcatccg
    22201 gccctttttt ctgatatgat acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg
    22261 gtaattcgca cgaaatttgt ttgtcggacg tagttcggat aaggcgttca cgccgcatcc
    22321 gacaaaacat ccggcacacc agacagcaaa agattttaaa acgttaattc acacccagga
    22381 gtgataaaga tgcaaaagca agctgagttg tatcgtggta aagcgaaaac cgtatacagc
    22441 acggaaaacc cggacctgtt ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc
    22501 gcgcgcattg agcagtttga tcgcaaaggt atggtgaaca acaagttcaa ttacttcatt
    22561 atgagcaaac tggctgaagc gggtatcccg actcaaatgg agcgtctgct ctccgatacc
    22621 gaatgtctgg tgaaaaagct ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct
    22681 gctggctctc tggtgaaacg tcttggaatc gaagaaggta ttgagctgaa cccgccgctg
    22741 ttcgatctgt tcctgaaaaa cgacgccatg cacgatccga tggtcaacga atcttactgc
    22801 gaaacctttg gctgggtgag caaagagaac ctggcgcgta tgaaagagct gacctacaaa
    22861 gcgaacgacg tgctgaaaaa actgttcgat gatgctggtc tgattctggt cgacttcaag
    22921 ctggagtttg gcctgtacaa aggtgaagtg gtactgggcg atgagttctc cccggacggc
    22981 agccgcctgt gggacaaaga aacactggag aaaatggaca aagaccgttt ccgccagagc
    23041 ctcggtggcc tgatcgaagc ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac
    23101 tgatttttct ggttgcttgt ttctttagcc ggagggtact ccggctaaat gtctttgata
    23161 cccccgctac aatacttctc attatctccc tttgacagga aagtcatacc aggccaatat
    23221 gtatatacat aacgtatatc ccaaaaaggg gggggctatg gaaactactg tatttttaag
    23281 taatcgcagt caggccgtca gattaccgaa agcggtcgct ctgccagaag acgttaagaa
    23341 agtagaaatt attgcgattg gtcgtacaag aatcatcact cccgccggtg agtcctggga
    23401 cagttggttt gatggcgaaa atgtgagtgc ggattttatg gatatccgtg atcaacccgc
    23461 aatgcaggaa agggaatcat tttgatgctg aaattcatgc tggataccaa tatctgtatt
    23521 ttcactatta agaataaacc agtacatgtc agagagcggt ttaacctgaa ttctggccgg
    23581 atgtgtataa gttctgtaac attgatggag cttatctatg gggcagaaaa aagtcagatg
    23641 ccagaacgca atttggcggt tattgaaggt ttcgtatctc gtttggtagt gcttgattat
    23701 gatagtgctg ctgcgacgca taccgggcaa atcagggccg agcttgcccg gcagggaagg
    23761 cctgttggac cttttgacca gatgatagcg ggacatgctc gcagtcgcgg tttgattgtg
    23821 gttaccaaca atacccgaga gtttgagcga gtggcgggaa tcagactaga agactggagc
    23881 taacattctc ttttacttct aacattcgta aattatataa cgtggcatga agccttaatt
    23941 cctccgccat ttcatcccgc tttcctgtgg taatcttcct ctgaaccacc tacgatcatt
    24001 tctataatga atatattgtt gaggtatcta tgcgttggca agggcgacgt gaaagtgaca
    24061 atgttgaaga caggcgcaac agctccggtg gtccgtctat gggcggtccc ggttttcgcc
    24121 tgccaagcgg taaaggcggg ctgattttac tgttagtcgt gctggttgca ggctactatg
    24181 gtgttgattt aaccgggtta atgactggac agccggtttc ccaacaacaa tcaacgcggt
    24241 ccattagccc taatgaagac gaagctgcaa aattcacctc ggtgattctg gcaaccacgg
    24301 aagacacctg gggacaacag ttcgagaaga tgggtaagac ctatcagcaa ccgaagctgg
    24361 tcatgtaccg tggaatgacg cgcaccggct gcggggctgg tcagtccatt atggggccgt
    24421 tctattgccc ggcggatggc acagtttata tcgatctctc cttctatgat gacatgaaag
    24481 acaaacttgg cgcggatggc gattttgccc aggggtacgt tatcgcccat gaagtcggtc
    24541 atcatgtgca gaaactgtta ggcatcgagc cgaaagttcg tcaactgcaa caaaacgcga
    24601 cgcaggcgga agtgaaccgc ttatctgtgc gtatggaact ccaggccgac tgttttgccg
    24661 gtgtctgggg gcatagtatg cagcagcaag gcgttctgga aaccggcgat ctggaagagg
    24721 cgctgaacgc ggcgcaggcc atcggcgatg accgtttaca acagcaaagt caggggcgag
    24781 tagtaccaga tagtttcaca catggcactt ctcagcaacg ctacagctgg tttaaacgtg
    24841 gtttcgacag cggcgatccg gcacaatgca atacttttgg taaaagcatt taacttccgg
    24901 ggcagggatg gctgaactga ctgcgcttca cacattaaca gcgcaaatga aacgtgaagg
    24961 gatccgccgc ttgctggtgt tgagcgggga agagggttgg tgttttgatc atgcgcttaa
    25021 gttgcgtgat gccttacctg gcgactggct gtggatttcg ccgcagccag atgctgaaaa
    25081 ccactgttca ccctcggcgc tacaaacttt acttgggcgc gagttccggc atgcggtatt
    25141 cgacgcccgc cacggctttg atgccgctgc ctttgcagca cttagcggaa cgttgaaagc
    25201 gggaagctgg ctggttttgt tactccctgt atgggaagag tgggaaaacc aacctgatgc
    25261 cgattcgctg cgctggagtg attgccctga ccctattgcg acgccgcatt ttgtccagca
    25321 tctcaaacgc gtacttacgg cggataacga tgctatcctc tggcggcaaa accagccgtt
    25381 ctcgttggcg cattttactc cccgtactga ctggcacccc gcgaccggcg caccacaacc
    25441 agaacaacag caactcttac agcagctact gaccatgcct ccgggcgtgg cagcggtaac
    25501 ggctgcgcgt gggcgcggta agtcggcgtt ggcagggcaa ctcatttctc gtattgcggg
    25561 cagtgcgatt gtcaccgcgc ccgcaaaagc ggcaacggat gtactggcac aatttgcggg
    25621 cgagaagttt cgctttattg caccggatgc cttgttagcc agcgatgagc aagccgactg
    25681 gctggtggtc gacgaagccg cagccatacc tgcgccgttg ttgcatcaac tggtatcgcg
    25741 ttttcctcga acgttgttaa ccactacggt gcagggctac gaaggcaccg gacgtggttt
    25801 tttgctgaaa ttttgcgctc gctttccgca tttacaccgt tttgaactgc aacagccgat
    25861 ccgctgggca cagggatgcc cgctggaaaa aatggttagt gaggcactgg tttttgacga
    25921 tgaaaacttc acccatacac cacaaggcaa tatcgtcatt tccgcatttg aacaaacgtt
    25981 atggcgaagc gagccagaaa cgccgttaaa ggtttatcag ttattgtctg gtgcgcacta
    26041 ccggacttcg ccgctggatt tacgccggat gatggatgca ccagggcaac attttttaca
    26101 ggcggctggc gaaaacgaga ttgccggagc gctgtggctg gtggatgagg ggggattatc
    26161 tcaacaactc agtcaggcgg tatgggcagg ttttcgtcgc ccgcggggta atctggtggc
    26221 ccagtcgctg gcggcgcacg gtagcaatcc actggcagcg acattgcgtg gacggcgggt
    26281 cagccggata gcagttcatc cggcgcgtca gcgggaaggc acagggcagc aacttattgc
    26341 tggtgctttg caatatacgc gtgacctcga ctatctttcg gtgagttttg gttacaccgg
    26401 ggagttatgg cgtttctggc accgctgcgg ttttgtgctg gtgcggatgg gtaatcatcg
    26461 tgaagccagc agcggttgct atacggcgat ggcgctgtta ccgatgagta atgcgggtaa
    26521 acaactggct gaacgtgagc attaccgttt acgtcgcgat gcgcaagctc tcgcaaagtg
    26581 gaatggcgaa acgcttcctg ttgatccact aaacgatgcc gtcctttctg acgacgactg
    26641 gcttgaactg gccggttttg ctttcgctca tcgtccgcta ttaacatcgt taggttgctt
    26701 attgcgtctg ctacaaacca gtgaactggc attaccggcg ctgcgtgggc gtttacagaa
    26761 aaacgtcagc gacgcgcagt tatgtaccac acttaaactt tcaggccgca agatgttact
    26821 ggtccgtcag cgggaagagg ccgcgcaggc gctgttcgca cttaatgatg ttcgcactga
    26881 gcgtctgcgc gatcgcataa cgcaatggca attttttcac tgactccttc aattttctcc
    26941 catggtcatt gtgagtcagc ggcgtaaaat taatgacatc agttaaggag ttcgccatga
    27001 aacatgacca ttttgttgtt caaagcccgg ataaacctgc gcaacagttg ttactgcttt
    27061 tccacggtgt cggggataac ccggtggcaa tgggggaaat aggtagctgg tttgcaccgc
    27121 tgtttcctga tgcgctggtg gtgagtgtcg gtggcgcgga gccgagcggt aatccggcgg
    27181 ggcgtcagtg gttttcagtg cagggtatta cggaagataa tcgccaggcg cgtgtggatg
    27241 ccatcatgcc gacgtttatt gaaacggtac gctactggca gaaacagagc ggagtagggg
    27301 ctaacgccac ggcgctaatt ggtttctcac aaggcgcgat tatggcgctg gagagcatta
    27361 aagccgaacc gggccttgct tcgcgcgtca ttgcctttaa cggacgctat gcgagcctgc
    27421 cggagaccgc gtctacagcc acgacaattc acttgattca tggtggtgaa gatccggtta
    27481 tcgatctggc gcatgcagtt gcggcgcagg aagcgttaat cagtgccggt ggtgacgtga
    27541 cgctggatat cgtggaggat ttaggccatg cgatcgacaa tcgcagtatg caatttgccc
    27601 tggatcactt gcgttatacc attccgaaac attatttcga cgaagcgcta agtggcggta
    27661 agcctggcga tgatgatgtg attgaaatga tgtaaaagac agattacctg gcctgataag
    27721 acgcgtgagc gtcgcatcag gcatcgttca ccattgccgg atgtggcgtt aacgccttat
    27781 ccggcctacg gttgagcact cagcttactt cttcggttga tcctttttcg gccagtcgtc
    27841 gtcatcgtcc cacttatcgt tgaaatcacg atgcggggga agctccggct tgttggcgag
    27901 aaatttcttg tggtcgacgc gcttgagatc tttaatcacg ttcagcagga cgcctaccaa
    27961 aaagacaatc accagaatcc accaatattt agccagccag tccatgctta tttcctcttg
    28021 cagaaccact catcaggcga cgagctgttc catgatacgt tgatacatac gggcaagtag
    28081 ctgcaggtcg gcagcgttca cacattcatt aattttatga atagtggcat tgaccggccc
    28141 gagttccacc acctgcgccc ccatgcgagc aataaagcgc ccgtcggacg ttccgcctgt
    28201 ggtcagtagc tgcggtttaa tttcattata gtgctcaacc gcgttaacga ccgcatccac
    28261 cagtttaccg cgcgcggtca aaaatggctg cccggaaagc caccagtcca ccgtataacg
    28321 cagttgatgt ttttcaagca gggcaagcac ctgtgctttg atcatctcat cagtcagttc
    28381 ggtgctgaag cggaagttaa actgcacaaa cagttcaccc ggaataacat tgttactgcc
    28441 cgttcccgcc tgaatattag caatctgcat actggtcgcc gggaaaaatt cattgccctg
    28501 atcccactca atagccacta attcattaag gaaaggtgct gcgcgatgta ctggattgtc
    28561 agccagatgc gggtaggcca catgcccctg aacgccatga atggtaaggt tgcaggttaa
    28621 cgatccgcga cgaccatttt tcaccacatc accaaccact tcgatactcg acggttcgcc
    28681 gaccaggcag taatcgagac gctcattacg tgccatcaac gcttcgacga cttttaccgt
    28741 accgttgtgg gcactggctt cttcatcaga ggtgatcaga aatgccagtc gccccgtatg
    28801 gttgggatgt tgtgcgacaa agcgttctgc cgctaccacc atcgccgcca gcgagccttt
    28861 catatctgcc gcaccgcgtc cgaataacat gccatcacga atggtgggtt caaacggcgg
    28921 attgatccaa cgatcggcgt cgccaggcgg caccacgtcg gtatgcccgg caaaggctaa
    28981 cgtttcacct tgcccacgcc atgcccaaaa attctgcgta tcggcaaagt ccatgcgttc
    29041 aacggtaaaa ccgatcgcct gcaaacgttc aatcaacaaa gcctggcatc ctgcgtcatc
    29101 aggactcagg gaagggcggc gaataagctg ttgtgtcagc tcaataaccg ggcacgacat
    29161 agactacacc tcatggaaaa attgctgata actggaatca ctgaaaccca gcagcatagg
    29221 cttaccaggc gcgcagagca atggacgttt gataattgca ggcatttcag tcattaatgc
    29281 cgccgcagag gccgcatcgg tgattttatt gcgggtggtt tcgtccagtt tacgccaggt
    29341 tgtaccacgg gtgttcagta acgcttccca gcctaattcg ttgataaaac cgttcaataa
    29401 ttcgctgtcc agcccatcga cgcggtaatc atgaaaacga tagtcgatgt tattggcttc
    29461 cagccaacgg cgagcctttt taatggtgtc acaatttttg atgccgtaaa gtgtaaccat
    29521 aatgaatcct ttttcgattt atgagaataa atactcattt aagggtgaaa acattttgcc
    29581 tgcaaatgat tatcaggaaa
//
LOCUS       DAZFXT010000056        29146 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.056, whole genome shotgun sequence.
ACCESSION   DAZFXT010000056 DAZFXT010000000
VERSION     DAZFXT010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29146
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.056"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            375..1241
                     /gene="folD"
                     /locus_tag="ACHKW0_002593"
     CDS             375..1241
                     /gene="folD"
                     /locus_tag="ACHKW0_002593"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HFO7475407.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            1243..1455
                     /gene="ybcJ"
                     /locus_tag="ACHKW0_002594"
     CDS             1243..1455
                     /gene="ybcJ"
                     /locus_tag="ACHKW0_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HFO7475408.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1563..2084
                     /gene="ybcI"
                     /locus_tag="ACHKW0_002595"
     CDS             1563..2084
                     /gene="ybcI"
                     /locus_tag="ACHKW0_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HFO7475409.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(2120..3505)
                     /gene="cysS"
                     /locus_tag="ACHKW0_002596"
     CDS             complement(2120..3505)
                     /gene="cysS"
                     /locus_tag="ACHKW0_002596"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HFO7475410.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3679..4173
                     /gene="ppiB"
                     /locus_tag="ACHKW0_002597"
     CDS             3679..4173
                     /gene="ppiB"
                     /locus_tag="ACHKW0_002597"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HFO7475411.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4176..4898
                     /gene="lpxH"
                     /locus_tag="ACHKW0_002598"
     CDS             4176..4898
                     /gene="lpxH"
                     /locus_tag="ACHKW0_002598"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HFO7475412.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKAEALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            5016..5525
                     /gene="purE"
                     /locus_tag="ACHKW0_002599"
     CDS             5016..5525
                     /gene="purE"
                     /locus_tag="ACHKW0_002599"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HFO7475413.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            5522..6589
                     /gene="purK"
                     /locus_tag="ACHKW0_002600"
     CDS             5522..6589
                     /gene="purK"
                     /locus_tag="ACHKW0_002600"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HFO7475414.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVMWAQSKFS"
     gene            complement(6726..7619)
                     /gene="ybcF"
                     /locus_tag="ACHKW0_002601"
     CDS             complement(6726..7619)
                     /gene="ybcF"
                     /locus_tag="ACHKW0_002601"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7475415.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVDPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQKALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKAYGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7616..8431)
                     /gene="ylbF"
                     /locus_tag="ACHKW0_002602"
     CDS             complement(7616..8431)
                     /gene="ylbF"
                     /locus_tag="ACHKW0_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HFO7475416.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     SHPQPLAAAWMQRAEETGLFGPLAMAASDPLTAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGTP"
     gene            complement(8442..9701)
                     /gene="ylbE"
                     /locus_tag="ACHKW0_002603"
     CDS             complement(8442..9701)
                     /gene="ylbE"
                     /locus_tag="ACHKW0_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HFO7475417.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(9711..11378)
                     /gene="fdrA"
                     /locus_tag="ACHKW0_002604"
     CDS             complement(9711..11378)
                     /gene="fdrA"
                     /locus_tag="ACHKW0_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HFO7475418.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDASLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAGTPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEMLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVACDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDNQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIRPLSPATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            11695..12744
                     /gene="allD"
                     /locus_tag="ACHKW0_002605"
     CDS             11695..12744
                     /gene="allD"
                     /locus_tag="ACHKW0_002605"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HFO7475419.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGFIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            12766..14001
                     /gene="allC"
                     /locus_tag="ACHKW0_002606"
     CDS             12766..14001
                     /gene="allC"
                     /locus_tag="ACHKW0_002606"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047652 - allantoate deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA];
                     GO_process: GO:0009442 - allantoin assimilation pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HFO7475420.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLNGTEYPQEVILSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDICIDIDLWMDEEPVPMNKDL
                     VATLTELCESEKLNYWVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            14012..14797
                     /gene="allE"
                     /locus_tag="ACHKW0_002607"
     CDS             14012..14797
                     /gene="allE"
                     /locus_tag="ACHKW0_002607"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071522 - ureidoglycine aminohydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0010136 - ureide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HFO7475421.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(14926..16071)
                     /gene="glxK"
                     /locus_tag="ACHKW0_002608"
     CDS             complement(14926..16071)
                     /gene="glxK"
                     /locus_tag="ACHKW0_002608"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HFO7475422.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LAANGGNLARVASIEMEECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(16093..17394)
                     /gene="ybbY"
                     /locus_tag="ACHKW0_002609"
     CDS             complement(16093..17394)
                     /gene="ybbY"
                     /locus_tag="ACHKW0_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HFO7475423.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(17451..18752)
                     /gene="allB"
                     /locus_tag="ACHKW0_002610"
     CDS             complement(17451..18752)
                     /gene="allB"
                     /locus_tag="ACHKW0_002610"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HFO7475424.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGEEAKREGRVTAHDYVASRPVFTEVEAIRRVLYLAKVAGCRL
                     HVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTDQFEEIGTLAKCSPPIRDLEN
                     QKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGGIAGLQNCMDVMFDEAVQKRG
                     MSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQPNSSYVLTNDDLEYRHKVSP
                     YVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILKHQQ"
     gene            complement(18812..20266)
                     /gene="ybbW"
                     /locus_tag="ACHKW0_002611"
     CDS             complement(18812..20266)
                     /gene="ybbW"
                     /locus_tag="ACHKW0_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HFO7475425.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGLGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(20436..21314)
                     /gene="glxR"
                     /locus_tag="ACHKW0_002612"
     CDS             complement(20436..21314)
                     /gene="glxR"
                     /locus_tag="ACHKW0_002612"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7475426.1"
                     /translation="MKLGFIGLGIMGTPMAINLARAGHQLHVTTIGPVADELLSLGAV
                     SVETARQVTEASDIIFIMVPDTPQVEEVLFGENGCTKASLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(21414..22190)
                     /gene="hyi"
                     /locus_tag="ACHKW0_002613"
     CDS             complement(21414..22190)
                     /gene="hyi"
                     /locus_tag="ACHKW0_002613"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HFO7475427.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYTPKTTTEAGLRWMDPYR"
     gene            complement(22203..23984)
                     /gene="gcl"
                     /locus_tag="ACHKW0_002614"
     CDS             complement(22203..23984)
                     /gene="gcl"
                     /locus_tag="ACHKW0_002614"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HFO7475428.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAKRPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVAECQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            complement(24074..24889)
                     /gene="allR"
                     /locus_tag="ACHKW0_002615"
     CDS             complement(24074..24889)
                     /gene="allR"
                     /locus_tag="ACHKW0_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HFO7475429.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(24967..25449)
                     /gene="allA"
                     /locus_tag="ACHKW0_002616"
     CDS             complement(24967..25449)
                     /gene="allA"
                     /locus_tag="ACHKW0_002616"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HFO7475430.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            25679..26605
                     /gene="allS"
                     /locus_tag="ACHKW0_002617"
     CDS             25679..26605
                     /gene="allS"
                     /locus_tag="ACHKW0_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HFO7475431.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YHPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPFGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            26674..27768
                     /gene="mnmH"
                     /locus_tag="ACHKW0_002618"
     CDS             26674..27768
                     /gene="mnmH"
                     /locus_tag="ACHKW0_002618"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043828 - tRNA 2-selenouridine synthase
                     activity [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HFO7475432.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            27884..28249
                     /gene="ylbG"
                     /locus_tag="ACHKW0_002619"
                     /pseudo
     CDS             27884..28249
                     /gene="ylbG"
                     /locus_tag="ACHKW0_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(29016..29146)
                     /locus_tag="ACHKW0_002620"
                     /pseudo
     CDS             complement(29016..29146)
                     /locus_tag="ACHKW0_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308588.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7222 a         7851 c         7197 g         6876 t
ORIGIN      
        1 ttaattgaaa atatgcactc tattctaaaa aatagagagc cccgttagat gaatacttcc
       61 gcgcaaaata tattcaacaa aaatatagac ctgaagcggt aaattaccag gctgaaaatt
      121 ctttttatat tgtcaggtat ttcttaaatt atcttaatcc ttaaacaagg aaataaatca
      181 gttccagatt tacaacgcca tcatggacga aaaatgaagc tttcaatctc agcgacctca
      241 gcgacggtgc gcctaacctt cgcaagaggt cgcttcacgc gataaatctg aaacgaaacc
      301 tgacagcgcg ccccgcttct gacaaaatag acgcatcccc ttcgacctac gtaacagatg
      361 gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg cagcaggtgc
      421 gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg gcaccaggac
      481 tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca agcaaacgca
      541 aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa accaccagcg
      601 aagcggagct gctggagctt atcgatacgc tgaatgccga caacaccatc gatggcattc
      661 tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa cgtattcatc
      721 cggacaaaga cgtggacggt ttccatcctt acaacgtcgg tcgtctgtgc cagcgcgcgc
      781 cgcgtctgcg tccctgcacc ccgcgcggta tcgtcacgct gcttgagcgt tacaacattg
      841 ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt cgcccaatga
      901 gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc actaaaaatc
      961 tgcgtcatca cgtagaaaat gccgatctgt tgatcgttgc cgttggcaag ccaggcttta
     1021 ttcccggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc aaccgtctgg
     1081 aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc gcctcataca
     1141 ttacgcctgt tcccggcggc gttggcccga tgacggttgc cacgctgatt gaaaacacgc
     1201 tacaggcgtg cgttgaatat catgatccac aggatgagta acatggcgac attttcttta
     1261 ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg gagcgaaagc
     1321 ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagtcgacgg tgcggttgaa
     1381 acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg tcacagcgta
     1441 caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc ttttcagact
     1501 tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc gaagcgacca
     1561 tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta gggttgggtt
     1621 cgaaagtcat ccctccgcgt ctgttatttg ccggaatcat cctggcgatg ctgcccgacg
     1681 ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt catcgcgggt
     1741 ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg attggacgcc
     1801 gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg ttgctatcgc
     1861 acagcttgct ggattcggta accactggcg gtaagggcgt tggctggctg tggccgtggt
     1921 cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt gcattgtctc
     1981 gttacaccac gccgtacggg catcaggtga taatttctga attgatgtgg gtgtggttgc
     2041 cggggatggt gcttatggga atgttgtggt ggcgcaggcg ataaccggat gcgaaaactc
     2101 gcatccggca acagcgcaat tacttacgac gccaggtggt cccttgcggg ccatcttcca
     2161 gcacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc cagtctttcg
     2221 ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact tcgctgtcgt
     2281 ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg cccaatacag
     2341 cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct tctgctttca
     2401 gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg gtgttgaaat
     2461 cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca ggcgcaacgg
     2521 ttttatctgt gccgcgcagc gcagtgtaga gacgctccag cgccgcacgc gcctgcttca
     2581 ggttctcttc gctgtagttc aactggctgc gatagtggcc cgacatcagg aagtaacgca
     2641 cggtttccgc gtcgtagtat ttcagcacat cgcgcacggt aaagaagtta cccagcgatt
     2701 tggacatctt ctcgcggtca accatcacca tccccgagtg catccagtag ttcacatact
     2761 ggccatcatg ggcacaggtg gactgcgcga tttcgttctc gtggtgcggg aacatcaggt
     2821 ctgaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc attgccgaac
     2881 attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc ggttcgccct
     2941 ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg accacgtcaa
     3001 cgcgcgcacc tgcctgcagc tggtcgagat cctgacgcga cagcacgcca tatgtcggat
     3061 cggtcgggac gtcgaacatc acgtcgccgt tgtccgccac ataagcgtga cctttggcga
     3121 tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc ggctccatat
     3181 ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc atgcgatcca
     3241 ccagcgccac aaagctttcg ccattttcat tggcgcgttt gatgattttg tcgtcgatat
     3301 cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag cgcgcaacca
     3361 cgtcaaaagc aacaaaggta cgcccgtgac cgatatgaca gagatcgtaa acggtgattc
     3421 cacacacata catgccgact tccccggcgt gaataggctt aaattcctct ttttggcgtg
     3481 tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg ggtaattgag
     3541 ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat gatccgatcg
     3601 tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag caccaatcaa
     3661 acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat tgtcatcaaa
     3721 acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg ccgcgaaggt
     3781 ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca gggcggcggt
     3841 tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga agccaacaac
     3901 ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc gcactctgca
     3961 actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc tggcgaaagc
     4021 ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga cgtggtagac
     4081 aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc aaaagaagac
     4141 gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt attgcagatc
     4201 ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt ttagcggggg
     4261 aagcccgcaa ggccgaagcg ctgtatattc ttggcgatct gtttgaagca tggattggcg
     4321 acgacgatcc caacccactc catcgccaga tggcggcagc gatcaaagcg gtgtccgatt
     4381 ccggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc aaacgctttg
     4441 cccgtgaaag cggcatgaca ttattgccgg aagaaaaggt gctcgaactt tatggtcgcc
     4501 gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat caggcttttc
     4561 gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg ttgtttgtgc
     4621 gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc agtaaatcgc
     4681 tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa catcaggtgc
     4741 aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc gccaaccagc
     4801 aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg gtgaaagtca
     4861 cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac tttgctgatt
     4921 tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt gccctctaaa
     4981 gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat aatccggcgc
     5041 gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc gccgccgaaa
     5101 tcttcgaaat cctgaatgtc ccgcaccacg ttgaagtggt ttctgctcac cgcacccccg
     5161 ataagctgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg attattgcgg
     5221 gcgcaggcgg cgcagcgcac ctgcctggta tgattgccgc caaaacgctg gtgccagtgc
     5281 tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac tccatcgtac
     5341 aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc gcggcaaacg
     5401 cggcgttact ggcagcacaa attcttgcga ctcatgataa agaactgcac cagcgtctga
     5461 atgactggcg caaagcccag accgacgaag tgctggaaaa cccggacccg cgaggtgcgg
     5521 catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc gccaggcagg
     5581 tgaaccgtta ggcattgctg tctggccggt cgggctggac gctgaaccgg cggcggtgcc
     5641 ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg cattaacccg
     5701 cgagctggcg cgtcatccgg cctttgtgaa ccgcgatgtg ttcccgatta ttgccgaccg
     5761 tctgactcag aagcagcttt tcgataagct ccacctgccg accgcaccgt ggcagttact
     5821 tgccgatcgc agcgagtggc ctgcggtgtt tgagcgttta ggtgaactgg cgattgttaa
     5881 gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgtgcca atgaaaccga
     5941 acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta acttctctgg
     6001 tgaagtgtcg ctggttggcg cacgcggatt tgatggcagc accgtgtttt atccgctgac
     6061 gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc aggccaatgc
     6121 gcagcagcaa gcgcaagccg aagagatgct gtcggcgatt atgcaggagc tgggctatgt
     6181 gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca acgaactggc
     6241 accgcgtgtg cataacagcg gtcactggac acaaaacggt gccagcatca gccagtttga
     6301 gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccggtagtga atagtccgtc
     6361 ggtgatgatc aacctgattg gtagcgatgt gaattatgac tggctgaaac tgccgctggt
     6421 gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc atctgaattt
     6481 gaccgacagc gacacatcgc gtctgaccgc gacgctggaa gccttgatcc cgctgctgcc
     6541 gccggagtat gccagcggcg tgatgtgggc gcagagtaag ttcagttaag caggtgctca
     6601 tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt acccattgta
     6661 ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc cggatgctct
     6721 catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc aattcgcgat
     6781 aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccacttac cgccgttacc
     6841 ttcggcccca tcgaaccata ggctttggca aatggcgcta actcatccgg tgtggcatgg
     6901 cgaatggcgc gttgctgcgg cgttccccag ttttcataaa ccgcgtcagc atcggtgagg
     6961 atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag atctttatca
     7021 atcaccgctt cactccctgc cccatcttcc gtcacaggca caccgccgcc gccactgcaa
     7081 atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag aattttacgc
     7141 ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat ctgccagccg
     7201 taagccgctt ccagtgcctt ttgttcttct ggctgataaa ccggaccaat aaatttctct
     7261 ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac cgtcgtgaca
     7321 ggcggcatct gcggctgtgc gctcaaactc tgcgccagca tatagccaat catcccctgg
     7381 ctttccgcca ccagcacatc cagcggatac ggatctacct ctttccacgc cagattctgc
     7441 aatgccagca gccccacctg cggcccattg ccgtgaacaa tcgccagccg ataagaacgg
     7501 gccaggcgcg ccagcgcggg tacagcactg gcgatattgc gatattgatt ttctgccgtc
     7561 agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag tgttttcatg
     7621 gtgttccttg taataattgt tgaccaagcc agaaccccag cagcgtatct gcccctgacg
     7681 tgtgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg caactcagag
     7741 catgaacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcgtag cgtaaataac
     7801 tgacgctcac cgctgtggta acgagttgaa gattgtcgga gcaggcaaaa aactggcgac
     7861 cggagcgcgc atctaaagcg ccataatacc aggccgccag cagcattccg ctcaacgtgt
     7921 catcatgact tggcgttaat ccggggcctt tacccagcca gtgccgccag tcggtcttaa
     7981 cgccattgag cgcggcctga aaacagtgac gaaactggcg taattcagcg gtcagcggat
     8041 cgcttgccgc catcgccagt ggtccgaaaa gtccggtttc ctccgcgcgt tgcatccatg
     8101 cagctgcaag cggttgagga tgtgaaggcg gcgtaatacg cagcaaacaa taacgctgtg
     8161 gctgtttaac cgtgaaacgc ccgaggcgaa tcccttgagc cacaacctgt ggtcgttcat
     8221 tgccgcataa tccaccgcat aacgcatcga attgcgcgcg gcgaagcacc catcctccgg
     8281 ggccgaaacc actaccctga cgatgcagcg ttaacagttc gccgctttcc gtcatcaggt
     8341 taatcgcccg tcgccacact cccgctaacc gccaggactg gcgataattc ggtgcactac
     8401 ggctggcaag cagaggatgg atgatcgtca ttgcgcgttc ctcaaccaat ccccatgctt
     8461 tcagccagtg cttccagcgc ctgttcaaaa cacgccagcg gtgcccgcac agtgcctgcg
     8521 ccaatctgcc cgatccccgc ctctttatgg gcgataccgg tattgatgag tggcgtaatg
     8581 ccggtttcta ccacgcgacg aatgtccagt cccaggcacg cgccctgaaa atcccagcct
     8641 gggatctgca actgcatatt gcgctcaagg taaatttccg ccatctcttc agataccgct
     8701 ctggccgctt ccatgccacc cgcaccgaca aagcgcgtta cgccaggcgc tgcgatcatt
     8761 gccgcacctc cgataccaaa ggtttcggta atcgcgctat cgcccatatc cgggttcgcc
     8821 tgctcctgcg agaagccggt gaaaaacaga ccttgcggag tgtttacagg cgcagtaaac
     8881 cagcgttcgc ccagcccgct tacccgaatc ccgaacatat tgccgttgcg ggtcattgcc
     8941 gtgacgatgc tgcctgcacg gatcatcgcg ccagcatcca tcgccgcctt gcagtaagcc
     9001 atcgcgaggt tgaggaagaa ctgatcggtc acgctgagga aatccatcac ttcggcgatg
     9061 tgctgtttat catgatcgag gcgagcaatt tgtggggcca gcgcacgcat taacagtgca
     9121 gaggaagcaa tattgcgttg atggaactca tcgcccatcg taatgccctg cgccatcatc
     9181 gccgtgagat cgataccgcg ctccatgcgc cccagcgccg cgcttaatac tggcattaac
     9241 acatcgcgca tccagcgatg gcgagtcagg acatcttcgc cgtaagcgcc aaaacgcatc
     9301 actttgccga taccttcgtt gaggttgcag tacgcccggt tgccgtcggt cacgttctcg
     9361 accaccagca tcggcatact ggcagaagta atgccgccca ttggcccgac ggcattcaca
     9421 tggtgacaag gaatgaagtt cacttccccc tgctccagta ttgccagcgc ctgcgcttca
     9481 tctttcgccc agccttcgaa cagacatgcg cccacgcacg cccctttcat gggtccggtc
     9541 atctcctgcc agcgcattgg cggcccggcg tgaagcagtg ttttgccctc gtttagttcg
     9601 ctgataagtg aagaagccgg ttgcacatcc agccagtgtg gacgagcgcg acgaatttgt
     9661 tcgataaccg cagcattggc ttgcgccact gatgtaaaca tgggaacccc ttattgcaaa
     9721 cgttctaata aacgagccag ttttttattg ccgccggcga ctggcgacca ttggtaatgc
     9781 acaaccggtt tgctggcgct ttgtagctcc agcgcaaagc tgcgtaatcc gatgttaatc
     9841 acggcgacgt tttccagtaa tgacggtgtg tgttgctgag tggcaggcga aagcggacga
     9901 atcagtgccg ccgccagcaa ggtggcttcc ggcagcgaac tcactaccgc gatccctgca
     9961 tcttccagcg tggcgatttg ctgcgagcgg cattgcgggt cacgttcagt gcctgtcacc
    10021 gtggcaatgg catacagtgg ttgattgtct gaacgcgcgg cacaggcttt ttgccaggcg
    10081 cttaccagcg aggcggcagg atcggcggtc gcaccgaagc caatcacgac atcaagcagc
    10141 aatacgcgca cttgcggttt agcgccgaga tcggcaatta actgattgcg taaggctggg
    10201 tcgatcatcg gatggggacg cccgacggtg tagaaatcat cgccgaggtc aataatctgg
    10261 tgaccatcgg cgtcgagcat catgccgtga tgatgggtat cgtcggcttc cacgccaagg
    10321 tgcccggcaa gtaatcccgc cgcttcggca gccagcgtac cgccggtata caaaccgcaa
    10381 ataaatccgc tgctggcagg cgttatcgcg ttacgtcgcg ccgtaacgcg tgaaagcagg
    10441 caagccaggc gtgcggcctc atccagcgag gaggcaaacc agacattctc gtcgcaggcc
    10501 accgccgggg tataacctaa aaacagcgcc accgtcggtt tgccggttgc tttcatggca
    10561 ttaacaattt tcagacgcac agcttcggca ggcggttttg aaacaaatgc cagcatttcg
    10621 cttttctcgt ctgcactgag catttccagc gccgtcagcg cactgatgcc gcccacttca
    10681 cggctgaggt cgcgcccgcc aaggccaatc gcgtgagtaa ttccctcccc tgccagcgca
    10741 atctgtgagc acagctcctg aatcccggta ccggaagcgc caatgacgcc aatgttgcct
    10801 tccggcatca cgttagcaaa agccagcggc gtgccggcaa tcatcgacgt accgcagtcc
    10861 ggccccatca ccagcaagcc tttttctcgc gcgcgggttt taagttggat ttcatcttcc
    10921 agcgtgacgt tatcggagaa catcatcacg ttgaggttgc gatccagcgc ctgattcgcc
    10981 agctccgccg catactcgcc agccactgaa atcagcgcca gactggcatc ggataatttc
    11041 tgacaggcac tgtcccagcg acgcacctgc gtcaaggact ggctgctgcc tgacccctgc
    11101 gccagttgtt ttagcgcctc ttcaagctgc tgcataatcg cctgcgcgat ccccgcatcc
    11161 gccgcttcgc tacgaattgc cacgcaaata tcgttcggcg tggcgttatt aaaatcgtca
    11221 tgccagaaac ctgtggtatc taataacgct ttattggcgg gcgtacccat cattacggaa
    11281 acatcatcaa cattttctga ttcgctgagt tttcgtgaaa taatcattaa actgaccgaa
    11341 tcctgaaaac accctttttt aataaaggcg tggatcataa caactcctta aaaattgcac
    11401 ccatggcaat tgcacgagca gtgttaatgt ggcgattacg gtatcgatga caacgggtaa
    11461 ataaggtgat gaatatcaca cagggattat taacaaaaga attcaaataa ccgccatgct
    11521 attacgtgtt attttttaat aagcgtggtt gttttaaaaa ccaggttaaa agcgagcgga
    11581 ttatcaaata aattctaact atattttttt gcctgtctgg atcacataat ccagatattt
    11641 tccctgtatg ttaattgcag tcatgcttca caccgtcgtt aaaaaggaag acagatgaaa
    11701 atcagtcggg aaacactcca ccagctaatt gagaataaac tctgccaggc tgggttaaaa
    11761 cgtgagcacg ctgcaaccgt ggctgaagta ttggtttacg ccgatgccag agggatccac
    11821 tctcatggcg cggtgcgcgt ggaatactac gcggaacgca tttcaaaagg cggcaccaac
    11881 cgcgaaccgg agtttcgtct tgaggaaacc gggccatgct cggcaatttt acatgccgac
    11941 aatgccgccg gacaggtcgc ggcgaaaatg ggtatggaac atgccatcaa aaccgcccag
    12001 caaaatggcg ttgcggtggt cggtatcagc cggatgggtc acagcggcgc aatctcttat
    12061 tttgtgcagc aggcagcccg cgccggattc attggcattt cgatgtgcca gtccgatcca
    12121 atggtggtgc cgtttggcgg cgcggaaatt tactacggta ctaaccccct ggcctttgcc
    12181 gcgccgggag aaggcgacga gatccttacc tttgatatgg cgactaccgt acaggcatgg
    12241 ggaaaagtgc tcgacgcccg ctcgcgtaat atgtctatcc cggatacctg ggcggtcgat
    12301 aaaaacggtg caccaacaac cgatccgttc gcggtacatg ctctgctccc cgccgccggg
    12361 ccaaaagggt atggcctgat gatgatgatt gacgtcctct caggcgtctt actcggctta
    12421 ccgttcgggc gacaggttag ttcgatgtat gacgatttac acgcagggcg taatttgggg
    12481 caattacata tagttattaa tccgaacttt ttctcctcca gcgaattatt ccgtcaacat
    12541 cttagccaga ccatgcgcga attaaatgcc attacccccg cgcccggttt taaccaagtt
    12601 tattatcccg gacaggatca ggatattaaa caacgcaaag ccgccgtcga aggcatcgaa
    12661 attgttgatg atatttacca gtatttaatt tccgacgcgc tttataacac gtcatacgaa
    12721 acgaaaaatc cctttgcgca ataattattg agacaggact ttcttatgat tacacatttt
    12781 cgtcaagcca tagaagaaac gctgccctgg ctttcctctt ttggcgctga cccaacgggt
    12841 gggatgaccc gtttacttta ttcgccggaa tggctggaaa cccagcagca atttaaaaaa
    12901 agaatggcag caagtgggct ggaaacacgt ttcgatgaag tagggaattt atacggtcgc
    12961 ctgaatggta ccgaatatcc acaggaagtg attctgagcg gttcgcatat cgataccgtg
    13021 gttaacggcg gtaacctcga cgggcaattc ggcgcgctgg cggcgtggct ggcaattgac
    13081 tggttgaaaa cgcaatacgg cgcgccgcta cgtacggtcg aagtggtggc gatggcagaa
    13141 gaagaaggca gccgcttccc gtatgtcttc tggggcagta aaaatatctt tgggctggcg
    13201 aatcctgacg acgtgcggaa tatctgtgat gccaaaggaa atagttttgt cgatgcaatg
    13261 aaggcttgcg gatttactct tccgaacgcc ccgctaactc cgcgtcagga tattaaagcc
    13321 tttgtcgaac tgcatatcga acagggctgt gtgctggaaa gtaatgggca gtcaattggc
    13381 gtggtgaatg caattgtcgg gcaacgtcgc tatacggtga cgctgaacgg cgaatcaaac
    13441 catgcaggca ccacgccgat gggttatcgt cgtgatacgg tttacgcttt cagtcgcatt
    13501 tgccatcagt cgatcgaaaa agcgaaaaag atgggcgatc cgctggttct gacctttggc
    13561 aaagtagagc cgcgcccgaa tacggtaaat gtggtgccgg gtaaaaccac gttcaccatt
    13621 gattgtcgtc ataccgacgc tgccgtgctg cgtgatttca cccaacagtt agaaaacgac
    13681 atgcgggcga tttgcgatga aatggacatt tgtattgata tcgatttatg gatggacgaa
    13741 gaacccgtgc cgatgaataa ggacctggtc gccaccctga cagaattgtg tgaaagtgaa
    13801 aaactgaatt actgggtgat gcacagtggt gccgggcacg acgcgcaaat tttcgcgcct
    13861 cgcgtaccaa cctgcatgat ttttatcccc agcatcaacg ggatcagcca taacccggcg
    13921 gaacgcacca atattaccga ccttgccgaa ggggtcaaaa cgttggcact catgctttat
    13981 caacttgcct ggcagaaata aggagtcata aatgggatat ttaaataacg tcaccggtta
    14041 ccgcgatgat ttactggcta accgtgcgat tgttaaacac ggtaatttcg cactgttaac
    14101 cccggatggt ctggtaaaaa atattattcc gggctttgaa aattgtgacg cgacaatcct
    14161 ctccacgcca aagctgggtg cctcttttgt tgattatctg gtcacactgc atcaaaacgg
    14221 tggcaaccaa cagggcttcg gtggcgaagg cattgaaacg ttcctgtatg tgatctctgg
    14281 aaatatcact gccaaagccg aaggcaaaac atttgcctta agcgaaggtg gctatcttta
    14341 ttgcccacca ggctccttaa tgacgtttgt taacgcccag gccgaagaca gccaaatctt
    14401 tttatataag cgccgctatg ttccggtaga aggccatgca ccgtggctgg tttctggcaa
    14461 tgccagcgaa ctggaacgca ttcactatga aggtatggat gatgtgatcc tgctggattt
    14521 tctgcccaaa gagttaggtt ttgatatgaa catgcatatc ctctcttttg caccaggtgc
    14581 cagccacggt tatatcgaaa cacatgttca ggaacacggt gcctatattc tttccggtca
    14641 gggggtttat aacctcgaca ataactggat cccggtgaaa aaaggcgatt acatctttat
    14701 gggcgcttat tctttacagg ctggttatgg cgtagggcgt ggtgaagcgt tcagctatat
    14761 ttactcgaaa gattgtaacc gcgacgtcga gatttaagtc ataccactcg cctgattatt
    14821 tttagctatg ttggattttg ccacggttta taccgtggct ttttttgtag taaattcagg
    14881 taaaaaaaga tgcgatataa atcgcatccg ctttaaaggt aagggtcagt ttttaattcc
    14941 ctgacctatt ttaatggcgc aggcaatatt tcgtgcgctg ttgaagagat tggtttcacc
    15001 gctggcgagc acttcggcta aaggtgccag acgtggcaaa atgctgaata ccgcatcaat
    15061 gccgtactgg tgcaccactt ccacgccatc acccaatacg ccagcaatcc caatcaccgg
    15121 gacattaaac tgcttcgcca ccgacgccac acccagcggc gctttaccgc ctgccgtttg
    15181 cgagtcgatg cgcccttccc cggtaatcac cagtgctgcg ccctgcactg cctgcgcaag
    15241 attgaccgca ttcaacacaa tctcaatacc tggtttaata tccgcattga gaaataccgc
    15301 cgccgcaata cccataccgc ccgcagcgcc gccgcccgcc atctggcaga cgttgatttc
    15361 agtttgctgt tgtaaaacac gggcgtaatt ttgcagcccc tgttccagct cttcgaccat
    15421 ttccggcgtt gccccttttt gcgggccaaa caccgccgcc gcgccgcgtg cccctaccag
    15481 cgggttatca acgtcacatg ctacttcaat atggcagttc gccagacgcg gatcgcattc
    15541 ctccatctca atgcttgcca cgcgcgctaa attgccgccg ttagctgcca gcacctgacc
    15601 gtcggcatca aggaaacgca caccgagcgc ctgcgccatg cccataccac cgtcgaccgt
    15661 cgcactgccg ccaatgccga gaataatatg gcgaatgccg ttatccagcg catgacgaat
    15721 taactccccc gtaccaaaac tggaggccag caacggatta cgcttttcag gcgcgaccag
    15781 catcaggcca cttgctgccg ccatctcaat caccgccgtt ttcccgtcgc cggtaaggcc
    15841 ataaaaagca ttcacttttt cgcccatcgg cccgcagact tcaagcgtca cgatgttgcc
    15901 gcccgtcgcg gcgaccatcg catccaccgt tccttcgccg ccatccgcta tcggcaaaca
    15961 gatatagttc gcatcgggaa agagggtcga aaacccggct ttaattgcct gacaacattt
    16021 ttctgcactt aagctctctt taaaagagtc tggcgcaatg acaatcttca ttatcaatcc
    16081 tttttcaaca aattattcga tacgttccca cggaataagg ttatccataa gaacagccag
    16141 taaaatcccg accaataagc cgttactgag cagaggacga agcgttaatg gaaggtcttg
    16201 cagatacaca ggcggtaatg ccattaaaaa tatgccgaca aataacggca atgcgagtcg
    16261 ataaatattg cgagcggtaa acgttatttg ctggctaaac accagcgcgg aaaagagtaa
    16321 aggcagataa gaaaccagca tgaccgcgct actcacgggt aaagggatac tgcaaaacag
    16381 tcgcgtgagt gccggaacca gcgccaccag caggcaaata acgctgccat aaataaacga
    16441 acgccgcgtg taatcgcctg tctgggttaa taaaccaatg gatgaaacaa acggtgaaaa
    16501 tggaattacc gccagcggta cggttatcag cgtcataaat ccggtcgcca caaagctgcg
    16561 acgataacgc gtatttcccg cgccctgctg cggataaaaa acatccgtgc cccgaatcgc
    16621 accgtaggta ttgctgatat tcaccagacc tgtaatcacc gccgtcagaa taattcccgg
    16681 agacaaagca cctccactgc cgagcgggaa ccactgccag tgcaactcac cggagagcga
    16741 gtgcgaagaa ggaaagcaaa agtaccacaa caaccagccg gttatggtgc cgaccagcag
    16801 gccataacgg gcaaaacgtt gcggcaggaa gataatcatc cccagtacca ggcacatcac
    16861 cgccaccgag agcgcgaacg gcggtaactg aattttaaaa tttgggtcgg ctatgccaaa
    16921 cggcagcccg agcatacctt tgaaaaagat agtggtcagc tgcgcgccca gcatcaacat
    16981 aaacaagacc atcaccgacg gcgtaaataa ccgtgccagg cgatggccta atccgctaaa
    17041 accaatcaac atcgtcagca cgccggagag cgcaatcccc accgccaggc tggtagcgat
    17101 atcgttgatc ggtgtcccgc gcgatgcttc gccaagggtg atagtaagga tggttcccca
    17161 ccacaggcca cccggccctt ccataatagc gcgacgatga ccgcagaacg cctgagcgaa
    17221 gcaggccagt gcggtagcaa gaaaagcata ttgcgtgagc gtaagcaggc tactttgcgg
    17281 caactgaaaa gcagaaagta gcgtaggagg aaccacaacc gtgttgcaaa aaataaagaa
    17341 aaaccactga aatcctgata acaggctttc gcggctcact gcaaaattga acataaacac
    17401 accttaaccg gagaatgccc gccgcaagga cgggcattgc aggggccaga ttactgctga
    17461 tgtttaagga taaattgacc tttcggcgca acagggaagc cctgttcaat gtcgtaaatc
    17521 acatcaccac gtaagatggt tttcgtgata cgcgcgccaa tggtacggcc aacatacggg
    17581 ctgactttgt ggcgatattc cagatcgtca ttggtaagaa catagctgct attcggctga
    17641 atgaagacga agtcggcatc ttttcctggg gcgatacggc ctttttgctg cagaccgaaa
    17701 atatctgctg cgttagtcgc cattaatttg ccgaacattg gcagagacat tccgcgtttc
    17761 tgtaccgctt catcgaacat cacgtccata cagttttgca gaccggcaat accgccccat
    17821 gcttccatga tgttgccggc tttcatttcc ggagggcatg gtgagtggtc ggaaaccagg
    17881 cagtcgattt caccgttaaa cagtttttcc cacatgcctt tctggttttc cagatcgcgg
    17941 atcggcggtg aacacttcgc cagagtgccg atttcttcga actgatcggt atccagtaca
    18001 aagtaatgcg ggcaggattc acaagtaacg tcctgacctt cctgacgtgc acgagtcact
    18061 tcttcaacgc cttccgggct gctgatatgg caaacgtgca gacggcaacc ggcaactttc
    18121 gccagataca gcacgcggcg aatcgcttcc acttcggtaa ataccggacg cgaagccaca
    18181 tagtcatggg cagttacgcg accttcacgt ttcgcttctt cgcccagctt ctgcgcacct
    18241 ttgaagaact gccagtcgtt tacatcacgg aagtcgttgt cgataccgcg atcgccacag
    18301 gtcgcaacga agcatttgaa gccgacaacg cccacttcat ccagctcatg cagacggtcg
    18361 atgttgtaag acaccaggcc accgagttgt gccgcatcga tagtcagctt gcctttagcg
    18421 gcatcgaact tcagttcaat tgaagcgcgg tcaaccgttg caggcagctg gttgagcggc
    18481 atttcgatca tggtggtgat accacctttt gccgctgcgc gagtaccggt ttcataacct
    18541 tcccagtggc tacgacccgg ttcagaaata tgggtgtgcg catcaaccat gcccggcgaa
    18601 accaccagac cagacgcatc cataacttct tttgcatcgc ccagatcctg accgatagca
    18661 gcaattttcc cgcctttaac ggcgatatct acaacgcgag cttcgttttc taaaataacg
    18721 gtgccgtttt taatgattaa atcaaaagac ataacaaact ccttgtaaaa tacagattgg
    18781 tttttaactc aatgttcaat atcgggatta attaacctat ggttttttgc tctcctgttt
    18841 tttctgctgt tgtacgtttc tttaataagg cgtaggccgc aaaggcgacg atgacgccga
    18901 caaaccatga aacacgcgat aacggttcca taaagggaat aaacttaccg ccaagagata
    18961 aaataacggc caccagagtt actgaaaacg cggtgaggtt aaaaccgtta tcgtaatatt
    19021 tataatcgcc aggtgcggtg tacagttcat caagattaat ttgcccacgc atcaccacaa
    19081 aataatgtgc catcatgaca ccaattaccg gacccagcat tccgccgatg atatcgagga
    19141 acagataaat actgtcctga ttttccatta atttccacgg gcagatcagc aggctgataa
    19201 tactggcaat cagtacgccg tttttatagg tcagttttgt cggtgcaatg gcagcaatct
    19261 gataaccggc cggaataata ttaccggtgg cgttagtgga aatagtcgtc atcagaataa
    19321 ccagtaccgc aaagaacgag gcaaacaggc tgtcccaacg ctgaacaata tccagcacgt
    19381 tccaggtgtc agcgccgtag tgaatactgg ctccggcaat aatacatacc ccagcgaccg
    19441 caaacagaat ataggccaca actaaaccca gcgtttgccc cagcgcttgc tcacgaaacg
    19501 agtgggcgtt ttgcgtaaag tcggatgcgc tcaccgccgg tgccgcccag accgcaacta
    19561 ccgcgttaat caccaccagg aacaagaatc cactgttttc tgctttctga ataccgctcg
    19621 gaatgtagtc aaagattgga ccgagcccga ccagcgaaat cgcccaaatc gccataccgc
    19681 cgaaaacgat atagatgcac gggttaagaa tggcagtgaa tttatttaaa actttgccac
    19741 cgccaaaacc tataccaacg ttgaccagcc agaagagtaa gaaagtaatt aagcccggta
    19801 gagaaagccc taacagcgtg aaatcaccac cgagagttaa aaatcccggc cagattttgc
    19861 caatcagaat caagcaggcc agtgaccccg cataacattg caggccaaac cacataatgg
    19921 cagcaatacc gcctcttaat aatccgggaa acagtgcgcc acgtacgcca taagaagcac
    19981 gcaggatcat ggcaaacggt acgccgtatt tactgcccgc agcgccgttt aataccatta
    20041 ccgcggcaat gaaaaaggcg ctgaggataa ttgccagcat aatactaaag gttgacaagc
    20101 cgagaataaa aaagccgccg accatcacat aattgggaac gttatgaacc gaacccatcc
    20161 ataaggtaaa atagttaaat gttttccagg ttcgctggct ttgcgttttc ggcaatagat
    20221 cttcgctata gccgcgttgc tggaatagtt ttctctgatg ttccataaat ccctcttaat
    20281 ttcacgtatc gaataacgtt gttttaatct agctatgagg aacaaataaa ataaaaatat
    20341 tatttttaat gctgaagcaa catctttggc tgctttcttt ttttaatatt gctggccgat
    20401 tcattttctg atatcggcca tttttattgc gggtatcagg ccagtttatg gttagccatt
    20461 aattccagcg cctgcactaa cgcagagtga tccaactggc tgccaccgtt tgccgcgcag
    20521 gtattaaata actcctggca ggtcgcagtg tttggcaggt tcagcgctag cgctttcgca
    20581 ctttgcagtg ccaggttgag atctttctgg tgcagcgcaa ttttgaaacc tggattaaaa
    20641 gtgcgtttaa tcatgcgctc accgtgaact tccagaatac gtgaggaagc aaagccgccc
    20701 atcagcgcct ggcgcacacg taccgggtcc gcaccggctt ttgaagcaaa tagcagggct
    20761 tcagaaaccg cttcaatatt gagcgccaca ataatctgat ttgccacttt gcaggtttga
    20821 ccatcgccgt taccgcccac gagggtgata tttttaccga gcagttcaaa cagcggttta
    20881 acacgttcaa ataccgcttc atcaccgcca accataatcg acaacgtccc ttcacgcgca
    20941 ccgatttcac cgccggagac tggcgcatcg agataatcgc cgcccagttc attcacctga
    21001 cgagcgaaac gcttagtttc aatcggggaa atggagctca tatcaacaat ggttttgccc
    21061 ttcagcgagg ctttggtaca accattttca ccgaacagaa cttcttcaac ctgtggtgta
    21121 tctggcacca taataaaaat gatgtccgat gcttccgtta cctggcgcgc agtttcaaca
    21181 ctgacggcac ccagtgacag taattcatca gcaaccggac caatggttgt gacatgcagt
    21241 tgatgaccgg cacgcgccag attaatggcc atcggtgtac ccataatgcc taagccaata
    21301 aatcccagtt tcataattaa cctcttttaa atttcgcttt tcctgaattc agacaacacg
    21361 atcccctgcc aacttagcgg cctaaaaaag cattgcctga acagcgttac gttttaacgg
    21421 tacggatcca tccagcgtaa accggcttcc gtggtggttt tgggtgtata ttcacaccca
    21481 acccaaccgt tgtagtcaga attttcgatt accttaaaga gataatcata attaatttct
    21541 ccggtccccg gttcgccgcg atgcggatta tcagcaattt gcaggtgacc aattttatcg
    21601 gcccactgag tcatggtgtt ggttaattca ccttccatcc gctgcatgtg ataaatgtca
    21661 tactgaattt tcaaattgca gcaaccaaca tcatcaatca atttcagcgc ctgccgagtt
    21721 ccggtgagat ggaaaccagg aatatcaaaa tggttaatag gttcaatcag taataaaata
    21781 tcttctttca tcagcatatt cgcggcataa cgcaggtttt ctacaagcgt tgcgtgaatc
    21841 tgttcactgc tgaaaccagc cggcgtttta ccgaccagac agttaatttt tttattaccc
    21901 agcgcacggg cataacgaat cgctgctgct acgccatccc gaaactcttc ttcacggcca
    21961 ggaatacagg caataccgcg ctcccccgcc gcccagtcac ctgccggtaa attgtgcagc
    22021 gtatgttcga gtttattact cgccagcacc tgttttaatt cttcaatgtc gtagtcataa
    22081 ggaaacataa attcaacgcc gcgaaaacca cactgcgcag ctttctcaaa acgggcgaga
    22141 aaatcatatt caccaaataa catcgataaa ttagcagaga aacgtaacat tatttatctc
    22201 ccttattcat agtgcatgaa gcaggtttca gtcggtgcat ccgctgcgtt atcggcgata
    22261 tcttcaaatt ccatgacgtt atccagttcg ctgcccatcg aaatattggt cacacgctcg
    22321 agaataactt ccacgactac cggtacccga tattgcgcca ttaaggcttt cgcctgttca
    22381 aaggctggcg caatatcttc cggtttgaag acgcgaatag ctttacaacc taaaccttcc
    22441 gctactttta cgtggtcaac accgtagcca ttcacttcac tggagttgat attctcgaaa
    22501 gcgagttgca cgcagtagtc catatcaaac gcgcgctgcg actggcgaat cagccccaga
    22561 taagcgttat tgaccagtac atggatgtac ggaatgttga actgtgcgcc aacggccaat
    22621 tcttcaatca ggaactggaa gtcgaagtca ccggaaatcg ccaccacttt gcgctctgga
    22681 tcagcggcac aaacccccag cgccgccgga atggtccagc ccagcgggcc tgcctgccca
    22741 cagttgatcc agtggcggtc tttaaagaca tgcagcattt gcgcagcggc aatttgcgac
    22801 agaccaatgg tggtgacata gcaaacatca cgtccaaaag ctttgttcat ctcttcatac
    22861 acacgctgcg gtttaaccgg aacgttgtcg aagtgtgttt tacgcaacaa agtacgtttg
    22921 cgctgctggc actcagcaac ccactcttta cggcatggca gacgccctgc tttttgcatt
    22981 tcctgcgcca cttcaaccag cagtgtcagc gccgctttag catcagagac aatccccaga
    23041 tccggacaca gcacgcggcc aatttgcgtc ggctcaatat cgatatgaac gattttgcgc
    23101 ccttcggtgt atttctctac cgaaccggta tgacggttag caaaacggtt accgatacca
    23161 aacaccatgt cggacgccag caacgttgcg ttaccgtaac gatgcgcggt ttgcagaccc
    23221 accatcccgg ccatcagttc atgatcgtcc gggatacagc cccagcccat cagcgttggg
    23281 atcaccggaa cgctggtcag ttcagcaaac tgttgtaaca gtgcagctgc gtcagcatta
    23341 attaccccgc ccccggcaac aatcaccgga cgtttggcct ggattaacat ttctacagct
    23401 ttttcgatct gcatacggct ggcagcaggt ttgtagaccg gcagcggttc gtacatatca
    23461 ggatcaaact cgatttccgc aacctgaacg tcgaacggta aatccaccag taccggtccc
    23521 ggacgaccag aacgcatcag gtgaaatgcc tgttgcagca cacgaggcac cagcgccgct
    23581 tcacgtactg taaccgccat tttgctgacc ggtttagcaa ttgcttcaat atctacggcc
    23641 tgaaaatctt ctttatgcag acgggcgcgc ggtgcctggc cggtaatgca cagaatagga
    23701 atggaatcag cagaagcgga atagagcgcg gtgatcatgt ccgtgcccgc aggaccggaa
    23761 gtccccagac atacgccgat attccctgcc gttgcgcggg tataaccttc cgccatgtgc
    23821 gaagcacctt ccacatgacg cgccagaatg tgacgaatac cgccgtgctt acgcatcgct
    23881 gagtagaacg gattgattgc agctcccgga acaccgaagg cggtggtgat accttctttc
    23941 tccagcacat acattgccgc gtcaacggct ctcatttttg ccattttatt cctacctcta
    24001 tttattggaa aatttttcca actttcaaat tttgtcaaaa aaacccgctc cgcagagcgg
    24061 gatgcgacag acattatgga tgtgctttca gtcccaacgc cgtgctgata tcgcgggcgg
    24121 tgtctctgac cagctcaccc tgactgacaa aacgatcttc tgtcagtctt gatgaaggcc
    24181 cggagatgga gatagcggca acaacgctac cgacatcatc gtaaattgct gaagctatgc
    24241 aattcagacc tacaacatgc tcttctttat ctacggtata gcccagttca cgcgcttgtt
    24301 ccaggtcctt cagcaaggtg ggcatatcca caagcgtagt tggcgtaaac tgctgcaaac
    24361 cggtttgcag aatgatgctc atcaactcct cttccgccag cggataaagc aacgccttgc
    24421 ccgcaccgga agcatgcagc ggcagacgac tgcccagtgg cgcacacatc ctgaccattg
    24481 atttacactc taactgacca attaatactg cttcattgcc gttacggatc gcgacattga
    24541 ccgtttcgcc ggaaagtaac atcaggcggc gcataaacgg cccggcgacg gagaggacat
    24601 cgcggttatg gatgtacgcc gcaccgacgt taaagacacc taatcctata tgccaccagc
    24661 ctaattgact gtcctgatag acaaaatccg ctgcttgtaa aaccttcagc aagcgaaagg
    24721 tcgtggagag cggcaaatcc agattgagag aaatatcgct aaccgacgaa cttcccccac
    24781 ttttttccag atattgcaga atcgcaattc cccgctctaa cgcctgtgcg cccttctgtg
    24841 ccacaggctc cgcctgtcct ggcctgccgc gccgtctaac ttccgtcata cctacgttaa
    24901 ctccgaccta caagtgagca taaacaatga acaattagtt tatcttatgc aagtcggttg
    24961 caggcgtcac gcaaaacaga gttcctgttc aggaatactt tcaacatcac agttgtcact
    25021 gccgccgcga tcgatggtca gaaaatcggt gacgcgctgc caggcgaaaa gtgggtgatg
    25081 ccagacgtta cgatggtaat tcactccctg ttcgccgttg gtgataaacg cccgcagcgt
    25141 tgacaggtct ggtttgtcgt cgcctaacgc cacgaccacc acaaacactt cacctttcat
    25201 cgggataaag gcctgagtgc ccagcggatg acgttcgagt tcgtaaatgg tcagcggcag
    25261 attcgccggt tgcgcgcggt taatgctgat aagcgtacgg tcttgctcaa gaatctcaac
    25321 cagcgccaaa tcgtggtaac gctccaccag gccattgtta atatggaaaa aatcccgttg
    25381 ctgagtttcg attacgtcgc cataagcact aaaggcttcc tgacttaacg gtaatacctg
    25441 aagtttcatc tttttgtcct tgtaacataa ttccgttcaa ccatcgccga ccggtcagct
    25501 tggtttcaat aacaggcaat ctaaaacctt aagagaacaa catcaataca aaagttggaa
    25561 tatttttcca aaactagatt tagtccacaa attgaacagg gttatcatgt aaaaatactg
    25621 ataaatcaga ggataagaaa atcacgcaac ttgcggattt aaaaggggat acctgaagat
    25681 gttcgatcca gaaaccttgc ggactttcat tgcggttgct gaaacaggaa gtttttcaaa
    25741 agcggcagaa cgattatgta aaaccacggc gacgatcagt tatcgcatta aacttctgga
    25801 agagaatacc ggagtagcgc tgtttttccg cacgactcgc agcgtgacgt tgacagcggc
    25861 tggcgagcat ctactttccc aggccagaga ctggctgagc tggctggaaa gtatgccaag
    25921 cgagctgcaa caggtgaatg atggcgtgga acgccaggtg aatattgtca tcaacaacct
    25981 gctctaccac ccccaggccg tcgcccagtt gctggcgtgg ctgaatgaac gttacccctt
    26041 tacccagttt cacatctccc gacaaatcta tatgggcgtc tgggattcgc tattatacga
    26101 aggtttttca ctggctatcg gcgtcacggg aaccgaggcg ctggcaaata cctttagtct
    26161 tgatcccttc ggatcggtgc aatggcgctt tgtcatggcg gcggatcatc cgctggcgaa
    26221 cgttgaagag ccgctaacag aagcgcagtt gcggcgcttt ccggcggtca atattgaaga
    26281 cagcgcccgc accttaacca aacgcgtcgc ctggcgattg cctgggcaaa aagagattat
    26341 tgttcctgat atggaaacga aaatcgccgc ccatcttgcg ggcgttggca ttggtttttt
    26401 gccaaaatcg ctttgccagt caatgatcga taatcaacaa ctagttagcc gggtaatccc
    26461 aacgatgcgc cctccttcgc cattgagtct ggcatggcgc aaatttggca gcggcaaagc
    26521 ggtagaagat attgtgacct tgtttaccca gcgcaggccg gaaatcagcg gatttttaga
    26581 aattttcggc aacccacgca gttaaaaatc aagtttcctt gcgcgttggc gcgtaaaata
    26641 gcgcgcaagc tgatttgagg ttcactttta actatgcaag agagacacac ggaacaggac
    26701 tatcgtgccc tgctgattgc tgatacgccc attattgatg ttcgcgcccc tatcgagttt
    26761 gagcaaggcg caatgcccgc cgctatcaat ctgccgttaa tgaataacga tgaacgcgcc
    26821 gccgttggca cctgctataa acagcaaggc tcagacgcag cgctggcgct ggggcataaa
    26881 ctggtggcgg gtgaaattcg tcagcagcgc atggacgcct ggcgggcagc gtgcctgcaa
    26941 aatccgcaag gtattctctg ctgcgcccgt ggcggtcagc gctcacatat tgtgcaaagc
    27001 tggttgtatg cagcggggat tgattatccg ctggtggaag gcggttataa ggcactgcgc
    27061 cagaccgcga ttcaggcgac tattgaactg gcacaaaaac cgatagtgct gattggcggt
    27121 tgtactggca gcggtaaaac gctgttagtg cagcaacagc cgaacggtgt tgatctggaa
    27181 gggctggcgc gccatcgcgg ttcggcgttt ggtcgcacgt tacaaccaca acttagccag
    27241 gcgagttttg aaaacctgct ggctgccgaa atgctgaaaa ccgacgcccg tcaggatttg
    27301 cgcctgtggg tgctggaaga cgaaagccgg atgatcggtt cgaatcacct gccggaatgc
    27361 ctgcgcgagc gaatgactca ggcggcgatt gcggtggtag aagatccgtt tgagatccgt
    27421 cttgagcgcc tgaacgaaga gtatttcttg cgtatgcatc atgattttac ccacgcttat
    27481 ggcgacgaac agggctggca ggaatattgc gaatacctgc atcacggact ttcggcgatt
    27541 aagcgtcggc tggggttaca gcgctataac gaactcgctg cccagctgga tacggcgctg
    27601 acaacgcaac tcaccaccgg cagcaccgac ggtcatctgg cctggctggt gccgttactt
    27661 aaagaatatt acgacccaat gtatcgctat cagcttgaga aaaaagcgga aaaagttgtc
    27721 tttcgcggtg agtgggcgga agtggcggaa tgggttaagg cgcggtaatg agggacgtat
    27781 ttgtattagt cgcgacttga tctgacacat ggccttgaaa ggttgagagt taccggtttt
    27841 gatatgagtg tcgaatcctt atccaaaaca agaggtaact ctcatgcttc atactgccaa
    27901 tcccgtcatc aaacacaaag ccggtttgct caatctggct gaagaactca gcaacgtatc
    27961 aaaagcctgt aaaatcatgg gcgtttcgcg tgatacgttt tatcgttacc gcgaactggt
    28021 cgctgaaggc ggtgtggatg cgcagattaa tcgtagtcgc cgcgctccta accttaagaa
    28081 ccgtaccgat gaggcaactg aacaggctgt tgttgattac gccgtcgctt tcccgacaca
    28141 tggtcagcac cgggccagca acgagctgcg taaacagggg gtttttatct ccgacagtgg
    28201 tgtccgttct gtctggttac tccataacct tgagaatctc aaaagacgat actgaacaaa
    28261 ttttatcagg tgacgttccg taaaaagttg tatggagttc tcgatgcagt cggcgcgact
    28321 atcagtaccc ccgctgaacc ctggactatc gggccgccgt actggggcat gtcaccctgg
    28381 cgtgccgctg gttttccgct aatggtttat ttcctctttc tttcccggca agaatttatt
    28441 ttaatccgga aggattattt attttcagat agggactcac attaaacacg taacatttta
    28501 attaatgcat ggctgaatat gtattacaca atgtcagata tcgtaaaaga ccagtgcaat
    28561 tctatgtaaa ctctctgact gttcatttta tttgttgttt cagggtcgga actcaccctc
    28621 aataaacaac tgaatacgag tagatattca tcgtctgagc tatatggctt tacacaatag
    28681 ccgacatgtc ttttctcagt caggaatttt acttttcctc actacgaagt tgacgacttc
    28741 gatatgggat agactcttaa ttcaagcaat tactctgcac aagcatcagg caatccagct
    28801 ccaatatttg ttataaaata atgagtaaca ttaatttcac caacaaaaaa aaggtttaca
    28861 ccatcccctt gccctggtat cttaccatat ttacaccaca ctttttttgt atctggatca
    28921 ttctttgata cgattttcgc cacttgttcc ctggccctca ctgtcaatgt tttcttaaac
    28981 ctacccccat catcatttgt tagggaaaaa taagaaaaaa aataatcatc ttctacattt
    29041 gtgtatattg atatatccac cgctacgtgc ggaaaaaaat ctgggagcca tgtaaaaact
    29101 ccatcatttt ctggtattgc catatagttt atgtaatgtt tactta
//
LOCUS       DAZFXT010000057        28577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.057, whole genome shotgun sequence.
ACCESSION   DAZFXT010000057 DAZFXT010000000
VERSION     DAZFXT010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.057"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            526..2949
                     /gene="pgaA"
                     /locus_tag="ACHKW0_002621"
     CDS             526..2949
                     /gene="pgaA"
                     /locus_tag="ACHKW0_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1901515 -
                     poly-beta-1,6-N-acetyl-D-glucosamine transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HFO7475433.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGALTVTQHTINTSPPFLRLMGTP
                     TSIPNDTWLQGHSFLSTVAKYSNDLPQAEMTARELAYNAPGNQGLRIDYASVLQARGW
                     PRAAENELKKAEVIEPRNINLEVEQAWTALTLQEWQQAAVLTHDVVEREPQDPGVVRL
                     KRAVDVHNLAELRIAGSTGIDAEGPDSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADG
                     QFSEGKGIVRDWLAGVEWRSRNIWLEAEYAERVFNHEHKPGARLSGWYDFNDNWRIGS
                     QLERLSHRVPLRAMKNGVTGNSAQAYVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLE
                     GQERIWSSPYLIVDFLPSLYYEQNTEHDTPYYNPIKTFDIVPAFEASHLLWRSYENSW
                     EQIFSAGVGASWQKHYGTDVVTQLGYGQRISWNDVIDAGATLRWEKRPYDGDREHNLY
                     VEFDMTFRF"
     gene            2958..4976
                     /gene="pgaB"
                     /locus_tag="ACHKW0_002622"
     CDS             2958..4976
                     /gene="pgaB"
                     /locus_tag="ACHKW0_002622"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HFO7475434.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            4969..6294
                     /gene="pgaC"
                     /locus_tag="ACHKW0_002623"
     CDS             4969..6294
                     /gene="pgaC"
                     /locus_tag="ACHKW0_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008375 - acetylglucosaminyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042710 - biofilm formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine synthase"
                     /protein_id="HFO7475435.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            6296..6709
                     /gene="pgaD"
                     /locus_tag="ACHKW0_002624"
     CDS             6296..6709
                     /gene="pgaD"
                     /locus_tag="ACHKW0_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HFO7475436.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(6759..7547)
                     /gene="phoH"
                     /locus_tag="ACHKW0_002625"
                     /pseudo
     CDS             complement(6759..7547)
                     /gene="phoH"
                     /locus_tag="ACHKW0_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
     gene            complement(8166..9437)
                     /gene="efeB"
                     /locus_tag="ACHKW0_002626"
     CDS             complement(8166..9437)
                     /gene="efeB"
                     /locus_tag="ACHKW0_002626"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0033212 - iron import into cell [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter
                     deferrochelatase/peroxidase subunit"
                     /protein_id="HFO7475437.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKPDLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(9443..10570)
                     /gene="efeO"
                     /locus_tag="ACHKW0_002627"
     CDS             complement(9443..10570)
                     /gene="efeO"
                     /locus_tag="ACHKW0_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HFO7475438.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAANVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(10628..11458)
                     /gene="efeU"
                     /locus_tag="ACHKW0_002628"
     CDS             complement(10628..11458)
                     /gene="efeU"
                     /locus_tag="ACHKW0_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake transporter permease EfeU"
                     /protein_id="HFO7475439.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(12001..13509)
                     /gene="putP"
                     /locus_tag="ACHKW0_002629"
     CDS             complement(12001..13509)
                     /gene="putP"
                     /locus_tag="ACHKW0_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005298 - proline:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0015824 - proline transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HFO7475440.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(13668..13877)
                     /locus_tag="ACHKW0_002630"
     CDS             complement(13668..13877)
                     /locus_tag="ACHKW0_002630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475441.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKINQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            13932..17894
                     /gene="putA"
                     /locus_tag="ACHKW0_002631"
     CDS             13932..17894
                     /gene="putA"
                     /locus_tag="ACHKW0_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7475442.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAAQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEAILSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLARRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPVERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(17934..18572)
                     /gene="rutR"
                     /locus_tag="ACHKW0_002632"
     CDS             complement(17934..18572)
                     /gene="rutR"
                     /locus_tag="ACHKW0_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HFO7475443.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            18860..19951
                     /gene="rutA"
                     /locus_tag="ACHKW0_002633"
     CDS             18860..19951
                     /gene="rutA"
                     /locus_tag="ACHKW0_002633"
                     /EC_number="1.14.99.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HFO7475444.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            19951..20643
                     /gene="rutB"
                     /locus_tag="ACHKW0_002634"
     CDS             19951..20643
                     /gene="rutB"
                     /locus_tag="ACHKW0_002634"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HFO7475445.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRNQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            20655..21041
                     /gene="rutC"
                     /locus_tag="ACHKW0_002635"
     CDS             20655..21041
                     /gene="rutC"
                     /locus_tag="ACHKW0_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-aminoacrylate deaminase"
                     /protein_id="HFO7475446.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            21049..21849
                     /gene="rutD"
                     /locus_tag="ACHKW0_002636"
     CDS             21049..21849
                     /gene="rutD"
                     /locus_tag="ACHKW0_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HFO7475447.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLQQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            21859..22449
                     /gene="rutE"
                     /locus_tag="ACHKW0_002637"
     CDS             21859..22449
                     /gene="rutE"
                     /locus_tag="ACHKW0_002637"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HFO7475448.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HSDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            22460..22954
                     /gene="rutF"
                     /locus_tag="ACHKW0_002638"
     CDS             22460..22954
                     /gene="rutF"
                     /locus_tag="ACHKW0_002638"
                     /EC_number="1.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent FMN reductase RutF"
                     /protein_id="HFO7475449.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            22975..24303
                     /gene="rutG"
                     /locus_tag="ACHKW0_002639"
     CDS             22975..24303
                     /gene="rutG"
                     /locus_tag="ACHKW0_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015218 - pyrimidine nucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006864 - pyrimidine nucleotide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HFO7475450.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGMNPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRKLVDVPPPEVVHQEP"
     gene            complement(24386..24559)
                     /gene="ymdF"
                     /locus_tag="ACHKW0_002640"
     CDS             complement(24386..24559)
                     /gene="ymdF"
                     /locus_tag="ACHKW0_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7475451.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            24932..25528
                     /gene="wrbA"
                     /locus_tag="ACHKW0_002641"
     CDS             24932..25528
                     /gene="wrbA"
                     /locus_tag="ACHKW0_002641"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HFO7475452.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPIATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            25549..25776
                     /gene="yccJ"
                     /locus_tag="ACHKW0_002642"
     CDS             25549..25776
                     /gene="yccJ"
                     /locus_tag="ACHKW0_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HFO7475453.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(25814..27055)
                     /gene="agp"
                     /locus_tag="ACHKW0_002643"
     CDS             complement(25814..27055)
                     /gene="agp"
                     /locus_tag="ACHKW0_002643"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HFO7475454.1"
                     /translation="MNKTLIAAAVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPYTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(27348..28577)
                     /gene="yccE"
                     /locus_tag="ACHKW0_002644"
                     /pseudo
     CDS             complement(27348..28577)
                     /gene="yccE"
                     /locus_tag="ACHKW0_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415521.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
BASE COUNT         7082 a         7320 c         7095 g         7080 t
ORIGIN      
        1 actgttgtca atatgaaatt ccgcccacaa acaataccga tgatgatagt tgctgaatgt
       61 aattattatt gcctaagtgc tttatacatt gtttgcaaca agccaggctt gaaaatactt
      121 ataaatggtt atagaaaatc cttataaaac attaattcat agatacatta ggaataacaa
      181 ttaaatccgt gagtgccgta gcgcagcctt tcatcaggac tttcgaaaaa tccgaaatca
      241 tgcatcggaa tttactgatt taattatttt aatcctaatt tattttgaaa aaggcattgg
      301 gatttatgcc gtattcctga agatcctcat cattggaatg gattttcggg cgagaaaagg
      361 attttatatg gacactctgc tcatcatttc ttcttctcat catcaacaat tcacgtctct
      421 cttccgcgtt taataacgga ttatgaggtg caaaaatatc tttcttttca gttacctgta
      481 attagataca gagagagatt ttggcaatac atggagtaat acaggatgta ttcaagtagc
      541 agaaaaaggt gcccgaaaac caaatgggct ttgaaacttc ttactgccgc atttttagca
      601 gcgagtcccg cggcgaagag tgctgttaat aacgcctatg atgcattgat tattgaagct
      661 cgcaagggta atactcagcc agctttgtca tggtttgcac taaaatcagc actcagcaat
      721 aaccaaattg ctgactggtt acagattgcc ttatgggccg ggcaagataa acaggttatt
      781 accgtttaca accgctaccg tcatcagcaa ttaccagcgc gtggttatgc agctgtcgcc
      841 gtcgcttatc gtaacctgca acaatggcaa aactcgctta cactgtggca aaaggcgctc
      901 tctctggagc cgcaaaataa ggattatcaa cggggacaaa ttttaaccct ggcagatgct
      961 ggtcactatg atactgcgct ggttaaactt aagcagctta actctggagc accggacaaa
     1021 gccaatttac tcgcagaagc ctatatctat aaactggcgg ggcgtcatca ggatgaatta
     1081 cgggcgatga cagagtcatt acctgaaaat gcatctacgc aacaatatcc cacagaatac
     1141 gtgcaggcat tacgtaataa tcaacttgct gccgcgattg acgatgccaa tttaacgcca
     1201 gatattcgcg ctgatattca tgccgaactg gtcagactgt cgtttatgcc tacgcgcagt
     1261 gaaagtgaac gttatgccat tgccgatcgc gccctcgccc aatacgctgc attagaaatt
     1321 ctgtggcacg ataacccaga ccgtactgcc cagtaccagc gtattcaggt tgatcatctt
     1381 ggcgcgttat taactcgcga tcgttataaa gacgttattt ctcactatca gcgattaaaa
     1441 aagacggggc aaattattcc gccctggggg caatattggg ttgcatcggc ttatctcaaa
     1501 gatcatcagc cgaaaaaagc acagtcaata atgaccgagc tcttttatca caaggagacc
     1561 attgccccgg atttatccga tgaagaactt gcggatctct tttacagcca cctggagagt
     1621 gaaaattatc cgggcgcgct aactgtcacc caacatacca ttaatacttc gccgcctttc
     1681 cttcggttaa tgggcacgcc tacgagcatc ccgaatgata cctggttaca ggggcattcg
     1741 tttctctcaa ccgtagcaaa atatagtaat gatcttcctc aggctgaaat gacagccaga
     1801 gagcttgctt ataacgcacc aggaaatcag ggactgcgca ttgattacgc gagtgtgtta
     1861 caagcccgcg gttggcctcg tgcagcagaa aatgaattaa aaaaagcaga agtgatcgag
     1921 ccacgtaata ttaatctgga ggttgaacaa gcctggacag cattaacgtt acaagaatgg
     1981 cagcaggcag ctgtcttaac gcacgatgtt gtcgaacgtg aaccgcaaga tcccggcgtt
     2041 gtacgattaa aacgtgcggt tgatgtacat aatcttgcag agcttcgtat cgctggctca
     2101 acaggaattg atgccgaagg cccggatagt ggtaaacatg atgtcgactt aaccaccatc
     2161 gtttattcac caccgctgaa ggataactgg cgcggttttg ctggattcgg ttatgccgat
     2221 ggacaattta gcgaaggaaa agggattgtt cgcgactggc ttgcgggtgt tgagtggcgg
     2281 tcacgtaata tctggctcga ggcagagtac gctgaacgcg ttttcaatca tgagcataaa
     2341 cccggcgcgc gcctgtctgg ctggtatgat tttaatgata actggcgtat tggttcgcaa
     2401 ctggaacgcc tctctcaccg cgttccatta cgggcaatga aaaatggtgt tacaggcaac
     2461 agtgctcagg cttatgttcg ctggtatcaa aatgagcggc gtaagtacgg tgtctcctgg
     2521 gctttcactg atttttccga cagtaaccag cgtcatgaag tctcacttga gggtcaggaa
     2581 cgcatctggt cttcaccata tttgattgtc gatttcctac ccagtctgta ttacgaacaa
     2641 aatacagaac acgatacccc atactacaac cctataaaaa cgttcgatat tgttccggca
     2701 tttgaggcaa gccatttgtt atggcgaagc tatgaaaata gctgggagca aatattcagc
     2761 gcaggtgttg gtgcctcctg gcaaaaacat tatggcacgg atgtcgtcac ccaactcggc
     2821 tacgggcaac gcattagctg gaatgacgtg attgatgctg gcgcaacgct acgctgggaa
     2881 aaacgacctt atgacggtga cagagaacac aacttatacg ttgaattcga tatgacattc
     2941 agattttaag gataaatatg ttacgtaatg gaaataaata tctcctgatg ctggtgagta
     3001 taattatgct caccgcgtgc attagccagt caagaacatc atttataccg ccacaggatc
     3061 gcgaatcttt actcgccgag caaccgtggc cgcataatgg ttttgtagcg atttcatggc
     3121 ataacgttga agacgaagct gccgaccagc gttttatgtc agtgcggaca tcagcactgc
     3181 gtgaacaatt tgcctggctg cgcgagaacg gttatcaacc ggtcagtatt gctcaaattc
     3241 gtgaagcaca tcgaggagga aaaccgctac cggaaaaagc tgtagtgctg acttttgatg
     3301 acggctacca gagtttttat acccgcgtct tcccaattct tcaggccttc cagtggcctg
     3361 ctgtatgggc ccccgtcggc agttgggtcg atacgccagc ggataaacaa gtaaaatttg
     3421 gcgatgagtt ggtcgatcga gaatattttg ccacgtggca acaagtgcga gaagttgcgc
     3481 gttcccgact cgttgagctc gcttctcata catggaattc tcactacggt attcaggcta
     3541 atgccaccgg cagcttattg cctgtatatg taaatcgtgc atattttact gaccacgcac
     3601 ggtatgaaac cgcagcagaa taccgggaaa gaattcgtct ggatgctgta aaaatgacgg
     3661 aatacctgcg tacaaaggtt gaggtaaatc cacacgtttt tgtttggcct tatggcgaag
     3721 cgaatggcat agcgatagag gaattaaaaa aactcggtta tgacatgttc ttcacccttg
     3781 aatcaggttt ggcaaatgcg tcgcaattgg attccattcc gcgggtatta atcgccaata
     3841 atccctcatt aaaagagttt gcccagcaaa ttattaccgt acaggaaaaa tcaccacaac
     3901 ggataatgca tatcgatctt gattacgttt atgacgaaaa cctccagcaa atggatcgca
     3961 atattgatgt gctaattcag cgggtgaaag atatgcaaat atcaaccgtg tatttgcagg
     4021 catttgctga tcccgatggt gatgggctgg tcaaagaggt ctggtttcca aatcgtttgc
     4081 taccaatgaa agcagatatt tttagtcggg ttgcctggca attacgtacc cgctcaggtg
     4141 taaacatcta tgcgtggatg ccggtattaa gctgggattt agatcccaca ttaacgcgag
     4201 taaaatactt accaacaggg gagaaaaaag cacaaattca tcctgaacaa tatcaccgtc
     4261 tctctccttt cgatgacaga gtcagagcac aagttggcat gttatatgaa gatcttgccg
     4321 gacatgctgc ttttgatggc atattgttcc acgatgatgc tttgctttca gattatgaag
     4381 atgccagtgc accggctatc acggcttatc agcaagcagg ctttagcggg agtctgagcg
     4441 aaattcgaca aaacccggag caatttaaac agtgggcccg ctttaaaagt cgtgcgttaa
     4501 ctgacttcac tttagaactt agtgcgcgcg taaaagccat tcgcggtcca catattaaaa
     4561 ctgcacgaaa tatttttgca cttccggtaa tacaacctga aagtgaagcc tggtttgcac
     4621 agaattatgc tgatttccta aaaagctatg actggaccgc tattatggct atgccttatc
     4681 tggaaggtgt cgcagaaaaa tcggctgacc aatggttaat acaattgacc aatcaaatta
     4741 aaaacatccc tcaggctaaa gacaaatcta ttttagaatt acaggcacaa aactggcaga
     4801 aaaatggtca gcatcaggct atttcttcgc aacaactcgc tcactggatg agcctattac
     4861 aactgaatgg agtgaaaaac tatggttatt atcccgacaa ttttctgcat aaccaacctg
     4921 aaatagacct tattcgtcct gagttttcaa cagcctggta tccgaaaaat gattaatcgc
     4981 atcgtatcgt tttttatatt atgtctggtg ttatgcatac ccctatgcgt agcgtacttt
     5041 cactctggtg aactgatgat gaggttcgtt ttcttctggc cgttttttat gtccattatg
     5101 tggattgttg gcggcgtcta tttctgggtc tatcgtgaac gccactggcc gtggggagaa
     5161 aacgcaccag ctccccagtt gaaagataat ccgtctatct ccattatcat tccctgtttt
     5221 aatgaggaga aaaacgttga ggaaaccata cacgccgctt tagcacagcg ttatgagaac
     5281 attgaagtta ttgccgtaaa tgacggttca acagataaaa cccgtgccat cctggatcgc
     5341 atggctgcac aaattcccca tttgcgggtc attcatctgg cgcaaaacca ggggaaagcc
     5401 attgcgctta aaaccggagc tgccgcggcg aaaagtgaat atctggtgtg cattgatggc
     5461 gatgcgttat tagaccgcga tgcggcggca tatattgtgg aaccgatgtt gtacaacccg
     5521 cgtgtgggtg ccgtaaccgg taatcctcgt attcgaacac gttctaccct ggtgggtaaa
     5581 attcaggttg gcgagtattc ctcaattatt ggtttgatca agcgaaccca gcgtatctat
     5641 ggaaacgtat ttaccgtttc cggtgttatt gccgcatttc gtcgcagcgc cctggcagaa
     5701 gtgggttact ggagtgacga tatgatcacc gaagatattg atattagctg gaagctgcag
     5761 ttgaatcagt ggacgatttt ttacgagcca cgggcgctgt gctggatatt aatgcctgaa
     5821 acgttaaaag ggctgtggaa acagcgcctg cgctgggctc agggcggtgc agaagtattc
     5881 ctcaaaaata tgacaaggtt gtggcgcaaa gaaaactttc gaatgtggcc gctgtttttt
     5941 gaatactgcc tgacgacaat atgggccttc acctgcctgg tcggtttcat tatttacgca
     6001 gtccaacttg ccggtgtacc gttaaatatt gaattgacac atatcgctgc gacacatact
     6061 gccggaatat tattgtgtac gttatgttta ctgcaattta ttgtcagcct gatgatcgag
     6121 aatcgctatg agcataatct gacttcatcg cttttctgga ttatttggtt cccggttatt
     6181 ttctggatgc tgagcctggc aacgacattg gtatcattta cacgagtcat gttgatgcct
     6241 aaaaagcaac gcgcccgttg ggtaagtccc gatcgcggga ttctgagagg ttaatatgaa
     6301 caatttaatt attacgaccc gacaatcacc agtacgttta ctggttgatt atgttgccac
     6361 aaccatcttg tggacattat ttgcgttgtt catattctta ttcgccatgg atctgctgac
     6421 gggttattac tggcaaagcg aggccagaag ccgacttcag ttctattttt tgctggcagt
     6481 ggcgaatgcc gtcgtgttaa ttgtctgggc gctgtacaat aagctgcgtt ttcaaaaaca
     6541 gcagcatcat gcagcctacc aatatacgcc gcaagaatat gcagagagct tagcaatacc
     6601 tgatgagctc tatcagcaac tacaaaaaag ccacaggatg agcgtacact tcaccagcca
     6661 ggggcaaata aaaatggttg tttcagaaaa agcgctagtc cgggcataaa cacccaaaac
     6721 aaagcccggt tcgcccgggc tctgcaccga taatacactt aactgtatgc atgcagcgta
     6781 cgttggcaaa gtgccgaacg tacgcagtcc tctttaccga accggacgat cccaaccatt
     6841 tcatcttctt cgaaacgttc cagcgcgtca cttaatccgg agcacacgcc gcgaggcaaa
     6901 tcgcattgcg tgatatcacc gttgacgata accgtcacgt tctccccgag gcgggttaaa
     6961 aacattttca tttgcgcggc agtcacattc tgcgcctcgt caagaatgac gactgcattt
     7021 tcaaaggtac gtccacgcat ataggcgaac ggcgcaattt ccaccttccc tatttccggt
     7081 cgcaggcagt actgcataaa ggaagcccct aagcgccgga ccagcacgtc gtagaccggg
     7141 cgaaaatagg gagcaaactt ttctgcgata tctccaggta agaagccaag atcttcatcg
     7201 gcttgcagaa ctggacgggt gacgataatc ctgtcgacat ccttatgtat cagggcctct
     7261 gccgcttttg ctgcgctgat ccaggttttt ccgcacccgg cttcgcccgt ggcgaatatc
     7321 aactgcttac tctcaatagc cttcagatag tgcaattgcg cttcatttcg cgcgaggatg
     7381 ggcgaagtat cgcgactgtc gcgggccata ccaatggctt ctacgccgcc catctgcaca
     7441 agcgaggtga cctattcttc ttcacgctgc ttatggctgc gcgaatcccg tctcagcaca
     7501 cgttttgcct cacgacgagc tttgatcact gctttttgtc ttcccatgga gagcaccttg
     7561 agttgtttgt attcatcaca cgcgccgttg gcagcgcgat tatgcgcacg aacatcagag
     7621 ggttggcttc cttgtaagcc atagtttgct tttggataaa atgccgaaaa acggctacgc
     7681 acaccgtttg cggcgtcggt aacacgagta aagagaggaa gtgagaaaaa tacagagtga
     7741 cgaagttgct accggaggag aaatctccgc aagtggtgga gcgtagctta acaagacatt
     7801 gtccagaaca ggacgttacc atccgcgatc tccatagtga ctgactatca ctgccgggaa
     7861 cttccgctgc tacttaataa ttacaacaga tctcgcattc attgcaacaa tatattcact
     7921 tatatttaac cataaaacac catttcagtg acattagttt ctactggaaa gatgacagag
     7981 tgatgacaga tatgaaaaaa gctgtgtgct ttcagcagga ttcgcatcag gcatctgcgc
     8041 acgaacgccg gatgcggcgt gaacgcctta tccgcctacc gaacccgcac cgattgtagg
     8101 cataagaaga cgcgcaagca tcgcatcagg catctgcgca agaacgcctt atccgtctat
     8161 aaacattaaa cccgcaataa cgcgcttccg agataatcgt tcgcgtcctt cactcccggc
     8221 agcgcaaaaa aataaccgcc gccgataggt ttaacgtatt cctccagcgc ttcgccattg
     8281 agcctttttt gtacagtcag gaagcctttt tccagatcgt gttggtaaca gacaaacagt
     8341 agccccatat ccagttgccc ggagttggtg acgcccagtg aataactgta gccacgacgc
     8401 agcatcaggc tggactcact ctccgccgtg cggggattcg ccagccggat atggctgtcc
     8461 agcgcaatca ccttcccttc cggatcgctg gcgtaatcgg gaacatcatg ctcatgctgc
     8521 attcccagcg gcgcaccggt ttgcttatca cgaccaaaaa tcgtctgctg ttctttcagc
     8581 ggagttctgt cccaaaattc cactcgaaac tgaatcaagc gtactgcctg atagctgcca
     8641 ccgattgtcc acgctggctc ctgctgatct gccgttaccc acaccacttt ttgcatcaac
     8701 ttatcattct ggctatcggg attggcagtg ccgtctttga aacccagcaa attaatcggc
     8761 gtctctttgc ctttactacg cgccgcgtga tcggaaataa acccttcccg cttccagcgc
     8821 acgctgagca aatctggcgt atgtttgatg atatcgcgca gcgcatggat caccgtgtcc
     8881 tgggtgttgg cgcaaatttg tagcaacaca tcaccatgac ataacgccgc atccagcgag
     8941 tcgttgggaa aacgcgtcat cttctgcaac tttttcggca tctgtggcgc aaggccaaag
     9001 cgctcatcaa acaatgagtg acccaccgat aacgtgatgg tgagattatc gggcgcaatg
     9061 tagccgccaa gaatgccgga atcgagtggt ggcaggcgcg gatttggcgt ttctggtgcc
     9121 gctccgccct gagtcagaaa agcaaaacgc tgagtcaaca agcgaaacaa ccgttcaaga
     9181 tcgggtttat cgctggcaag cacatcaaac gccaccagca tcattgcggc ctgttgtggc
     9241 gtcaggatcc ctgcctgatg ctcaccataa aacggctgtt tctcattgcg tgcatccggt
     9301 gaaagcgtac ccggcgcact ttgcgttttt tgtgcatgag cgaccggaca acttcccgcc
     9361 agcgccagcg caccaatgcc tttcagtaaa cgtcggcgtg acggttcatt cacgccgttt
     9421 ttatcttcat actgcataat gcttaatcca atcccagcac accgcgaagt tgcgccagat
     9481 cttccgccag cgcagtaatc ggtcctttca gtgcattccg gtcggcatcg gtcaatttgt
     9541 cgtaggtttc aaaaccgtct ttagtacggt atttcgccag aatggtatcg acctttttaa
     9601 agttggcatc gacttttgcc aacagttccg ggttggcttt ttgcagttgt ggacgcagca
     9661 gatcgacaat tttctgcgag ccttcaacgt tagcctggaa atcccacaga tcggtgtggc
     9721 tgtagcgatc ttcttcaccg ctgattttgc tggctgccac ttcctcaatc agtccggctg
     9781 caccgccgac cacttttgaa ggtgggaaag ccagttcact gatgcgtttt tgcaaatcga
     9841 ccacatcggt ataaagctgg tcagcgtact gatccatgcc tttggtggtg ttgtcgccaa
     9901 acaatgcttt ttccagacgg tggaaaccgg tgaatttcgg atcggcggct ttttgctcgt
     9961 aatcatcttc acgggcgtca atgctgccat ccagatcgga gaacagttca gcaatcggtt
    10021 cgatgcgctc atagtgctgg cgcgtcggcg catacagtgc tttcgctttt tcgatatcgc
    10081 ctgctttaat agcgtcagta aaggctttcg tgtcggtcac cagctgcgtg gtttccgcca
    10141 tgacatacgc tttatatgca gtaattgcac caccaagact taacagcgca tcactttgcg
    10201 ccgcatccgc cgttgcctca cctttgacga tcaacttccc tttcgggtta gtcagcagac
    10261 cgcaggtcat atcgtattcg ccaggctgta aattcgccgt cattttctgg ctaaagccag
    10321 gggcgatatt ttcccgctct tccaccacca tcacgccttt gaggatctcc cactccagcg
    10381 ccttctggct gtggttctga ataatgaact gtgttttccc ggcgttaacc gtaatggtca
    10441 tcggttcgca ctgcttatcg gtcacggtca ccttgacctg cggcacatta gcggcgttag
    10501 ccataaaagc agaagagaac agcgcagcca cgctcaactg caatgcgtta cggcggaagt
    10561 taatggtcat gacgagacta tccctttaaa gtatgttgta actaagcaag agtttgcgtc
    10621 gtatttgcta cgcggagcga gacgctgtcg cccctgcgcg tggtggcagg gcaaatgcca
    10681 ccagcgccgg gatgagataa ataaaccaga cggcgacttc gctgacgctc ggtgcttcct
    10741 gatagccaaa aatcccttcc atcagcgtgc caaacagcga gtgagttgag aggaccgcgc
    10801 tcatatcgaa ggcgatttcc tgaaagtggt tccacaatcc ggcttcatga aatgcgcgaa
    10861 tggcaccagc cgccagccct gcggcgacga agagaataaa caggctggtc catttaaaaa
    10921 atgcaccaag attgaggcga ataccgcccc agtagagcag gaagccgagc accacggcag
    10981 tagcaagacc gagcattgca cccagcggcg gccagatccc gacatcttgt tgaaatgccg
    11041 ccagcaggaa aaagaccgac tccagccctt cccttgcaac ggcaaaaaag accatcatca
    11101 ccagcgccca gccatgatga tttccacgct gcaatgcgct atcgactgcc tgttccagtt
    11161 gcactttgac gttgcgcgac actttgcgca tccagaaaac catccaggta aggatcacca
    11221 cggcgatcac cgccacgata ccttcaaaca gttcctgttc tttttgcgga aattcgccgg
    11281 tggtttcgtt aatgaagata cccaggccca ggcacaacgc agcggcaagc aacacgccaa
    11341 tccacatcac accaatccat cggcctcgct gggtacgctt aaggtagctg gcaatcaaac
    11401 tgacaatcag cgcggcttca agtccttcgc gcaacataat gagaaacgga acaaacatgc
    11461 cacccaccct taaacgtttt tctgagtcaa tctatgcaaa gaaaagtaaa tcgcagtgat
    11521 agcgattatc attacggtaa agaaaaatac aagcggaatg tttataaaat gatgttggaa
    11581 tgtaaacaaa gagaggtaat gccaagggat ataaacgatt attccagggt agaaagatgg
    11641 gattgtttgc aaagaaccat gtgttcttgc cggatgccgc gtaaacgccg tatccagccg
    11701 acaaaactgt ccagactcaa tgcatcagag cttccatgta aaactgataa gcgggtgtat
    11761 caagcaattt cgtccatccg cgattatcaa atctaacttt ttgcccggaa cataataaca
    11821 gctcgggtgt tggtggcgtc aaaatcgctg aagcgttccc cgaaggccgg gacagcaggg
    11881 ggcggcggcg attggccgcc ccctgcgcgc tccctgcacc attcaattta tatggcgagg
    11941 cacattaagg gagcgcaacc cgctgctgaa gccaaacaaa accgccgcag gctaagtccc
    12001 ttagctttcc tgcaaccgtg acggcggagc cgaatgatag tgcgcatcgg cctcggcaaa
    12061 gcgtttttgc atcgccgctg acggcgcttt acccagcaaa ctaaacacta caatcccaat
    12121 actgccgaag ataaagcccg gaataatttc gtagagtccc agccagccga actgcttcca
    12181 gacgataacc gtcagcgcac cgatgatcat ccccgccagc gcaccgttgc gcgtcatgcg
    12241 tgaccacatc accgagaaca gcaccaccgg accaaacgcc gcgccaaagc ctgcccacgc
    12301 gtagctcact aagcccagca cgcggttttc cgggtttgcc gccagcgcaa tcgccaccag
    12361 cgccaccacc agcaccatca cacgccctac ccacaccagc tctttctggc tggcatgttt
    12421 acgcagaaac gctttgtata aatcttcggt aatcgcactg gagcacacca gcagctggca
    12481 gcttaacgtt gacattaccg ccgccagaat tgccgacagc agaatcccgg caatccacgg
    12541 gttaaacaga atttgcgcca gttcgataaa cacacgttca gcgttctggt ttaccgcacc
    12601 tgctaccgcc gggtgctcat taaagtaagc aatcccaaag aagccgacag ccaccgcccc
    12661 tgccaggcag aggatcatcc aggtcatact gatacgacgc gcatggacaa tgctgtggtg
    12721 agaatccgcc gccataaaac gcgccagaat atgaggctga ccgaagtaac ccagccccca
    12781 acccatcaat gagataatgg caacaaagtt cagccctttg agcatatcca cgttttcgat
    12841 gctcttttgt ttgatcactt ccagcgagtc accaaagcca ccgacactga taatgacgat
    12901 aaccggtgtc aggatcaggg caaaaatcat caggctggcc tgtacagtat cagtccagct
    12961 caccgcgagg aaaccgccaa taaaggtgta aaggatcgtc gccgcagctc ccgcccacag
    13021 agccgtttcg tagctcatgc caaaggtact ttcaaacaga cgcgcgcctg ccacaatgcc
    13081 cgaagcgcaa taaatggtga agaacagcaa aataaccagc gcggagataa tgcgcaaaat
    13141 gcggctttta tcttcaaagc gcccggtgaa ataatccggc agggttaagg cgttattgtt
    13201 gtattcggta tgcacacgca accgcccggc caccagcttc cagttgatcc acgcgcctaa
    13261 tgtcaggcca atggcgatcc agctttcgga aatcccggaa agaaaaaccg cgcccggcaa
    13321 ccccattaac agccagccgc tcatatccga cgcacccgcc gataatgccg tcacgaatgg
    13381 cccaagacta cgaccgccca gaatatagtc gtcaaagttt ttcgttgatc gccaggcgat
    13441 aaacccaatc aatatcatgc caaagatata gacacaaaat gtcaccaaca tcggtgtgct
    13501 aatagccatc taaagtctcc aaaaaattat tatcggcaat gtcgaaactt gccgttatat
    13561 ctgccaccgg aacggggtaa cagagtttat gttttaccag ggcgaccgta tcctgccgga
    13621 agcgctggtt attcacaatc gatttaacac accatttaca ttaaatttta gtgctcagcg
    13681 acactatttt tcatcaggtt gcactctctc acattttttg cggttgcacc tttcaaaaat
    13741 gttaactgcc gcagagaaaa agtctgattt atttttttaa tccctgtcat atcgatttct
    13801 tttattaaca tttcattcat ttttaagctt gctacgcagg tcacatttaa catggttgca
    13861 caaagttgca acatcatgga tatttcacga taacgttaag ttgcaccttt cagaacaaca
    13921 ggagtaatgg catgggaacc accaccatgg gggttaagct ggacgacgcg acgcgtgagc
    13981 gtattaagtc tgccgcgaca cgtatcgatc gcacaccaca ctggttaatt aagcaggcga
    14041 ttttttctta tctcgaacaa ctggaaaaca gcgatactct gccagagcta cctgcgctgc
    14101 tttctggcgc tgccaatgag agcgatgaag caccgactcc ggcagaggaa ccacaccagc
    14161 cattcctcga ctttgccgag caaattttgc cccagtcggt ttcccgcgcc gcgatcaccg
    14221 cggcctatcg tcgcccggaa accgaagcgg tttctatgct gctggaacaa gcccgcctgc
    14281 cgcagccagt tgctgcacag gcgcacaaac tggcgtatca actggccgat aaactgcgta
    14341 atcaaaaaaa tgccagtggt cgcgcaggta tggtccaggg gttattgcag gagttttcgc
    14401 tgtcatcgca ggaaggcgtg gcgctgatgt gtctggcaga agcgttgttg cgtattcccg
    14461 acaaagccac ccgcgacgcg ttaattcgcg acaaaatcag caacggtaac tggcagtcac
    14521 acattggtcg tagcccgtca ctgtttgtta atgccgccac ctgggggctg ctgtttactg
    14581 gcaaactggt ttccacccat aacgaagcca tcctctcccg ctcgctgaac cgcattatcg
    14641 gtaaaagcgg tgaaccgctg atccgcaaag gtgtggatat ggcgatgcgc ctgatgggcg
    14701 agcagttcgt cacgggtgaa accatcgcgg aagcgttagc caatgcccgc aagctggaag
    14761 agaaaggttt ccgttactct tacgatatgc tgggcgaagc tgcactgact gccgcagacg
    14821 cacaggcgta tatggtttcc tatcagcagg cgattcacgc catcggtaaa gcgtctaacg
    14881 gtcgtggcat ctatgaaggg ccgggcattt caatcaaact gtcggcgctg catccgcgtt
    14941 atagccgcgc ccagtatgac cgggtaatgg aagaacttta cccgcgtctg aaatcactca
    15001 ccctgctggc gcgtcagtat gatattggta ttaacatcga tgccgaagag gccgatcgtc
    15061 tggagatctc cctcgatctg ctggaaaaac tctgtttcga gccggaactg gcaggctgga
    15121 acggcatcgg ttttgttatt caggcttatc aaaaacgctg cccgttggtg attgattacc
    15181 tgattgatct cgccacccgt agccgtcgcc gtctgatgat tcgtctggtg aaaggcgcgt
    15241 actgggatag tgaaattaag cgtgcgcaga tggacggcct tgaaggttat ccggtttata
    15301 cccgcaaggt gtataccgac gtttcttatc tcgcctgtgc gaaaaaactg ctggcggtgc
    15361 cgaatctaat ctacccgcag ttcgcgacgc acaacgccca tacgctggca gcgatttatc
    15421 aactggcagg tcagaactac tatccgggtc agtacgagtt ccagtgcctg catggtatgg
    15481 gcgagccact gtacgagcag gtcaccggga aagttgccga cggtaaactt aaccgaccgt
    15541 gtcgtattta tgccccggtt ggcacacatg aaacgctgct ggcgtatctg gcgcgtcgcc
    15601 tgctggaaaa cggtgctaac acctcgtttg ttaaccgtat tgccgacacc tctttgccac
    15661 tggatgaact ggtcgccgat ccggtcactg ctgtagaaaa actggcgcaa caggaagggc
    15721 aaactggatt accgcatccg aaaattcccc tgccgcgcga tctttacggt cacgggcgcg
    15781 acaactcggc agggctggat ctcgctaacg aacaccggct ggcctcgctc tcttctgccc
    15841 tgctcaatag tgcactgcaa aaatggcagg ccttgccaat gctggaacaa ccggtagcgg
    15901 caggtgagat gtcgcccgtt attaaccctg cggaaccgaa agatattgtg ggctttgtgc
    15961 gtgaagccac gccgcgtgag gtagaacagg cgctggaaag tgcggttaat aacgcgccaa
    16021 tctggtttgc cactcctccg gttgaacgcg cggcgatttt gcatcgcgct gccgtgctaa
    16081 tggaaagcca gatgcagcaa ctgattggta ttctggtgcg tgaagccgga aaaaccttca
    16141 gtaacgccat tgccgaagtg cgcgaagcgg tcgattttct ccactactat gccggacagg
    16201 tgcgggatga tttcgctaac gaaacccacc gtccattagg gcctgtggtg tgtatcagtc
    16261 cgtggaactt cccgctggct attttcaccg ggcagattgc tgccgcactg gcggcaggta
    16321 acagcgtgct ggcaaaaccg gcagaacaaa cgccgctgat tgccgcgcaa gggatcgcca
    16381 ttttgctgga agcgggtgta ccgccaggcg tggtgcagtt gctgccaggt cagggggaaa
    16441 ccgtgggcgc gcaactgacg ggtgatgatc gcgtacgcgg ggtgatgttt accggttcaa
    16501 ccgaagtcgc tacgttactg cagcgcaata tcgccagccg cctggacgct cagggtcgcc
    16561 ctattccgct catcgctgaa accggcggca tgaacgcgat gattgtcgat tcttcagcac
    16621 tgaccgaaca ggtcgtcgtg gatgtactgg cctcggcgtt cgacagtgcg ggtcagcgtt
    16681 gttcggcgct gcgcgtgctg tgcctgcaag atgagattgc cgaccacacg ttgaaaatgc
    16741 tgcgcggcgc aatggccgaa tgccggatgg gtaatccggg tcgcctgacc accgatatcg
    16801 gtccggtgat tgatagcgaa gcgaaagcca atattgagcg ccatattcag accatgcgta
    16861 gcaaaggccg tccggtgttc caggcggtgc gggaaaacag cgaagatgcc cgtgaatggc
    16921 aaagcggcac ctttgtcgcc ccgacgctga tcgaactgga tgactttgcc gaattgcaaa
    16981 aagaggtctt tggtccggtg ctgcatgtgg tgcgttacaa ccgtaaccag ctaccagagc
    17041 tgatcgagca gattaacgct tccggctatg gtctgacgct tggtgtccat acgcgtattg
    17101 atgaaaccat cgcccaggtc actggctcgg cccatgttgg taacctgtat gttaaccgta
    17161 atatggtggg cgcagtggtg ggtgtgcagc cattcggcgg cgaagggttg tccggtaccg
    17221 ggccgaaagc aggcggtccg ctctatctct accgtctgct ggcgaatcgc ccggaaagtg
    17281 cgctggcagt gacgctcgcg cgtcaggatg cagagtatcc ggtcgatgca cagttgaaag
    17341 ccgcattgac tcagccgcta aatgcactgc gggaatgggc agcaaatcgt ccagaattgc
    17401 aggcgttatg tacgcaatat ggcgagctgg cgcaggcagg aacacaacga ttgctgccgg
    17461 ggccgacggg tgaacgcaac acctggacgc tgctgccgcg tgagcgcgtg ttgtgtattg
    17521 ccgatgatga gcaggatgcg ctgactcagc tcgccgccgt gctggcggtg ggcagccagg
    17581 tactgtggcc ggatgacgcg ctgcatcgtc agctagtaaa ggcattgcca tcggcagtca
    17641 gcgaacgtat tcaactggcg aaagcggaaa atataaccgc tcaacccttt gatgcggtga
    17701 tcttccacgg tgattcggat cagcttcgcg cattgtgtga agccgttgct gcgcgggatg
    17761 gcgcaattgt ttcggtgcag ggttttgccc gtggcgaaag caatatcctt ctggaacggc
    17821 tgtatatcga acgttcgctg agtgtgaata ccgctgccgc tggcggtaac gccagtttaa
    17881 tgactatagg ttaaacaggt agccggagga tgttacaacc tcctccggca tctttaacgt
    17941 ggtcgaatcc cctcaataat aatccgctgc acgttttcaa ccgtttgatt gaaaaatatc
    18001 tcatcgcgca acgtcgcgcc tgtcaccgcc tccacctgag gggcgaaatc ggcgtaatgt
    18061 tgagtggaag cccaaatcat gaaaatcaaa tgctgcggat caatcggcgc gagtttgccg
    18121 cttttgaccc aaccggcaat cagcgccgat ttctcatcaa ttaatgcctt caaatcgccc
    18181 gtcagttcat ccattaacag cggcgcgcct gccagcatct ccatacagaa caggcgcgaa
    18241 gcctgcggat aatcgcggga gacttccagc ttcagacgga tgtactcttt tatcgccgcc
    18301 agcggagcga aatcttcacg aaacgctttt aacggtgcca gccagatatc gagaatctgc
    18361 cgcagcacgg caatatacag cgcctctttt gacggaaagt aatacagcag attggttttt
    18421 gaaacacccg ccaactctgc gatctgctcc agccttgtgc cgtgaaaacc gaattgtgaa
    18481 aaagtgtcca gtgctgcgct aagaatcgct tttttcttcg cgcttactgc gcgcgaacgt
    18541 ttacccgttg ttttcactgc gccttgcgtc atgcgctctc ccctcttgta gattggcaac
    18601 aggatagcaa aacccccgcc cggcctcgac cttctgcact ctcatcgcgc acagatgcat
    18661 gttttatgtg caactgtttt gaccgtttag tccacttttt accagatatt ttagataacg
    18721 cattgttaac atattaacaa cattaacttt ttaaaaactg gcatccgctt tgcaaacaag
    18781 ccattacgcg gctgaaaagg atatgcagga tgcagcgccc cgtctgactt tcactttacg
    18841 agacgaagag aggttgatga tgaaaattgg cgtattcgta cctattggca acaacggctg
    18901 gctcatttcg acccacgcgc cgcagtacat gccgaccttt gaactgaata aagccattgt
    18961 gcaaaaagcg gagcactacc atttcgattt cgccctgtcg atgatcaaac tgcgtggctt
    19021 tggcggcaaa actgagttct gggatcacaa ccttgagtcg ttcaccttga tggcggggct
    19081 ggcggccgtc acctcgcgca ttcagattta cgccactgct gccaccttaa cgttacctcc
    19141 ggcaatcgtc gcccgtatgg ccgcaaccat cgactccatc tctggcgggc gttttggcgt
    19201 caacctcgtg actggctggc aaaagcccga gtatgagcag atgggcatct ggcctggcga
    19261 tgactatttc tcccgtcgtt acgactatct caccgaatat gttcaggtgc tgcgcgacct
    19321 gtggggcacg gggaaaagcg attttaaagg cgattttttc accatgaatg attgtcgcgt
    19381 cagtccgcaa ccgagtgtcc ccatgaaagt gatctgcgcc gggcaaagcg acgctggcat
    19441 ggcgttctcc gcccagtatg ccgatttcaa cttctgtttc ggcaaaggcg taaatacgcc
    19501 cacggctttc gccccgaccg ctgcgcggat gaaacaggcc gcagagcaaa ccgggcgcga
    19561 cgttggttct tatgtgttgt ttatggtgat tgccgatgaa accgacgatg ccgctcgcgc
    19621 caaatgggaa cactacaaag cgggcgcgga tgaagaggcg ttaagctggc taaccgaaca
    19681 aagtcagaaa gatacccgct ccggtactga taccaacgtc cgtcagatgg ccgatcccac
    19741 ttcggcggta aacatcaata tggggacgtt agtcggttct tacgccagtg tcgcgcgcat
    19801 gttagatgaa gtcgcaagcg tgcctggtgc cgaaggcgtg ctgttgacct tcgatgattt
    19861 tctgtcggga atcgaaacct tcggcgagcg cattcaacca ctgatgcagt gccgcgccca
    19921 tctacctgcg ctgactcagg aggtggcatg atgacaacct taaccgctcg accggaagcc
    19981 attaccttcg atccgcagca aagtgcgctg atcgtggtgg atatgcaaaa cgcctatgcc
    20041 acgccaggcg gctacttaga tctcgccggg tttgatgtct caaccactcg cccggtcatt
    20101 gccaacattc aaaccgctgt gaccgcagcg cgagcggcag ggatgttgat catctggttt
    20161 caaaatggct gggatgaaca gtatgtcgag gctggcggcc ccggctcacc gaattttcat
    20221 aaatcgaacg ccctgaaaac catgcgtaat cagccgcagc tgcaggggaa attgctggcg
    20281 aaaggctcct gggattatca actggtggat gaactggtgc cgcagcctgg cgatattgtg
    20341 ctgccgaagc cgcgctacag cggtttcttc aatacaccgc tggacagcat tttgcgcagc
    20401 cgtggaatac gccatctggt tttcaccggt atcgctacca acgtctgcgt cgaatcgacg
    20461 ctacgcgatg gcttttttct ggagtatttc ggcgtggtgc tggaagacgc aacacaccag
    20521 gcggggccgg aatttgcaca gaaagccgcg ttgttcaata tcgaaacctt ttttggctgg
    20581 gtcagcgacg tcgaaacgtt ctgcgacgcg ctttctccca catcctttgc tcgtatcgct
    20641 taaggagttt aacgatgcca aaatccgtaa ttattcccgc tggcagcagc gcaccgctgg
    20701 cccccttcgt tcccggcacg ctggctgatg gcgtggtgta tgtctccggt acgctggctt
    20761 ttgatcaaca taataacgtg ctgtttgccg atgacccaaa ggcgcaaacc cgccacgttc
    20821 tggaaactat ccgcaaggtg atcgagacgg cgggtggcac gatggcggat gtgaccttca
    20881 acagcatctt tattaccgac tggaaaaatt acgccgcgat taacgaaatc tacgccgagt
    20941 tttttccggg tgataaaccg gcgcgattct gcattcagtg cggactggta aaacctgacg
    21001 cgctggtgga aatcgccaca attgcgcata tcgccaagtg aggccgcgat gaaactttca
    21061 ctctcacctc ccccttatgc tgatgcgccc gtagtggtgt tgatttcggg tcttggcggt
    21121 agcggcagtt actggttaca gcaactggcg gtgctggagc aggagtatca ggtagtctgt
    21181 tacgaccagc gcggcaccgg caataatccc gacacactgg cagaagatta cagtatcgcc
    21241 cagatggcag cggaactgca tcaggcgctg gtagccgcag ggattgagca ttacgcggtg
    21301 gtcggccatg cgctcggtgc gctggtggga atgcagctgg cgctggatta tcccgcgtcg
    21361 gtaactgtgc tgatcagcgt taacggctgg ctacgaataa acgcccatac gcgccgctgt
    21421 tttcaggttc gcgaacgatt actgtatagc ggcggcgcgc aggcatgggt ggaagcgcag
    21481 ccgttgttcc tctatcccgc cgactggatg gcggcccgcg cacctcgcct tgaggcagaa
    21541 gacgcgctgg cactggcgca ttttcagggc aaaaataatt tactgcgtcg acttaacgcc
    21601 ctcaaacgcg ctgactttag tcaccatgcg gatcgcatcc gctgcccggt gcaaatcatc
    21661 tgcgccagtg atgatctgct ggtgccaaca gcatgttcca gtgaacttca tgccgccctg
    21721 cccgatagcc agaaaatggt gatgccctat ggcggacacg cctgcaacgt gaccgatccc
    21781 gaaacgttta atgctctgtt actcaacggg cttgccagcc tgttacatca ccgtgaagcc
    21841 gccctgtaag gaattgctat gaacgaagcc gttagcccag gtgcgcttag caccctgttc
    21901 accgatgccc gcactcacaa cggctggcgg gagacacccg tcagcgatga gacgttacgg
    21961 gagctttatg ccctgatgaa atgggggccg acatcagcta actgttctcc ggcacggatc
    22021 gtgtttatcc gcactgcaga aggaaaagaa cgtctgcgcc cggcgctttc cagcggcaat
    22081 ctacaaaaaa ccctgaccgc gcccgtcacc gctatcgtcg cctgggacag tgaattttat
    22141 gaacggttac cactactgtt tccccacagt gatgcccgca gttggtttac ctccagccca
    22201 caacttgccg aagaaacagc gtttcgcaac agttccatgc aggcggccta tctgatcgtc
    22261 gcttgccgtg cgctgggact ggataccggc ccgatgtcgg gttttgaccg tcaacacgtt
    22321 gacgacgcct tttttgcggg cagcacgctg aagagcaatc tgctgattaa tatcggctat
    22381 ggcgatagca gcaaactttt tgcgcgcctg ccacgtctgt cctttgaaga agcctgcggg
    22441 ctgttgtaag gagcaaacta tgaacatagt tgatcaacaa acctttcgcg atgcgatgtc
    22501 ctgtatgggc gcggcggtca atatcatcac cacggacggt ccagccgggc gcgccgggtt
    22561 caccgccagc gccgtctgca gcgtgaccga tacgccgccc acattactgg tgtgcctgaa
    22621 tcgtggggcg tccgtctggc cggtattcaa tgaaaatcga acgctgtgtg taaatacact
    22681 tagcgccggg caggagccgc tttccaacct ttttggcggc aaaacgccca tggaacaccg
    22741 ctttgccgcc gcccgctggc agacgggtgt gaccggatgc ccgcaactgg aagaggccct
    22801 ggtttcgttt gactgccgta tcagccaggt ggtgagcgtc ggcacccacg acattctgtt
    22861 ttgcgccatc gaagcgattc atcgtcacgc cacaccctac gggctggtgt ggttcgatcg
    22921 cagttatcac gcgctgatgc gccctgcttg ttaacctcct taaggagaca gctgatggca
    22981 atgttcggtt ttcctcactg gcagttaaaa tcgacctcta cagagagcgg cgtggtcgcg
    23041 ccggatgaac ggttgccgtt tgctcagacg gcagtaatgg gcgttcaaca cgcggtggcg
    23101 atgtttggcg cgacggtgtt aatgccgatt ctgatggggc tggatcccaa tctttccatt
    23161 ttaatgtcgg ggatcggcac gttactgttt ttctttatta ccggcgggcg cgtccccagt
    23221 tatctcggct ccagcgcggc ttttgtcggc gtggtgatcg ccgcgacggg ctttaacggt
    23281 cagggcatga acccgaatat cagcatcgcg ctgggcggga ttatcgcctg tgggctggtt
    23341 tataccgtca ttggcctggt ggtgatgaaa atcggtacac gctggattga acggctaatg
    23401 ccgccggtgg tgacgggtgc ggtggtgatg gcgattggcc tgaacttagc gcctatcgca
    23461 gtgaaaagtg tatcggcttc ggcctttgat agctggatgg cggtaatgac ggtgctgtgt
    23521 attgggctgg tggcggtgtt tacccgaggc atgatccagc ggttattgat tctcgtagga
    23581 ttaattgtcg cctgtctgct gtatggcgtg atgaccaacg ttttagggct gggaaaagcc
    23641 gtcgatttta ctctcgtcag ccatgctgcc tggttcggtt tgccacattt ttccactcct
    23701 gcttttaatg gtcaggcaat gatgctgatt gcgcccgtgg cggtgattct ggtggcggaa
    23761 aacttaggtc atctcaaggc ggtcgccggg atgaccggac gcaatatgga cccgtatatg
    23821 gggcgagcat tcgtcggtga tggtctggca acgatgcttt ccggctctgt cggcggtagc
    23881 ggcgtcacca cctatgctga aaatatcggt gtgatggccg tgacaaaagt ctactcgacg
    23941 ctggtgtttg ttgctgctgc cgtcatcgcc atgctgttgg gcttttcgcc gaaatttggc
    24001 gcgctgatcc ataccattcc ggccgcggtg attggtggtg catcaattgt tgtgttcggg
    24061 ctgattgccg tcgcaggcgc aaggatatgg gtacaaaacc gtgtcgattt aagccagaac
    24121 ggtaatttga ttatggtcgc agtaacctta gtgctgggcg cgggtgattt tgctctcacg
    24181 ctgggcggtt ttacgttggg agggattggt acagcaacct ttggcgcgat tttactcaat
    24241 gcgttgttaa gcagaaagtt ggttgacgtt ccaccgccag aagttgttca tcaggagcca
    24301 taaacagaaa accgccggga ttaccggcgg tgatgacttt ttgacgagcc taagaaaaaa
    24361 atgcattcgt cattgattag cccggctagt tgtcgctttt gccgtgactg ctcttgccac
    24421 cttttttacc tgcttcagat gcgcgctgcg ggtcattctt gaaattaccc ccgctgtgct
    24481 gtccaccttt tttacctgct tctgatgctc tttcgcggtc ttctgcaaaa ttgccggaac
    24541 cgcctcgatg gtttgccatt actgacctcc gtctgggtat agacatcata ttgcatagaa
    24601 ttaacgcggc gtatggcggc tgccatattt tcgctacgtg ggactaagct tagtgaactg
    24661 acgcgggcag acccgcgttt ggtaatatcc tgcaacagct tcttagcgag aatagatatc
    24721 atctcaaata attatccata agccgctctt atgattaaaa aaacgcgatg agataaaaat
    24781 gtgtcatttt gcgacaaaat tacgtgcttg tgaaagttgt tataaatcaa ataagtggtt
    24841 gtgaaatttg cactctgaaa aggacgtctt atctttaaat aagtggtagc gaatcgctac
    24901 ggaatagaga taacacgagg agtggttaga aatggctaaa gttctggtgc tttattattc
    24961 catgtacgga catattgaaa cgatggcacg cgcagtcgct gagggtgcaa gcaaagtcga
    25021 tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat tatttgaaaa
    25081 agcaggcggt aaaacgcaaa ctgcaccgat tgcaaccccg caagaactgg ccgattacga
    25141 cgccattatt tttggtacac ctacccgctt tggcaacatg tccggtcaaa tgcgtacctt
    25201 cctcgaccag acgggcggcc tgtgggcttc cggcgcacta tacggaaaac tggcgagcgt
    25261 ctttagttcc accggtactg gcggcggtca ggaacaaact attacttcaa cctggacgac
    25321 ccttgcgcat cacggcatgg taattgtccc cattggctac gcagcgcagg aattatttga
    25381 cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc accatcgcag gcggtgacgg
    25441 ctcacgccag ccaagccagg aagaactgtc tattgctcgt tatcaagggg aatatgtcgc
    25501 aggtctggca gttaaactta acggctaatc ttcaacagga ggatacgcat gccaactcaa
    25561 gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc gccggaaata
    25621 gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa tggcggaaaa gatttgggaa
    25681 gaaggtagcg atgaagtgtt agtcaaagcc tttgctaaga ccgataaaga ttcgcttttt
    25741 tggggcgaac agaccatcga acgtaaaaac gtttaaccag cgactccccc gcttctcgcg
    25801 ggggagtttt ctgttatttc accgcttcat tcaacacgct atcaaattta tccatcgggc
    25861 agaaaccgtt ggcatctatc gggcaaccgc ttaattccag cgtcacacgc tgagcaggtg
    25921 cctgcagggt taacgcatcg gcattacgta actgttccgc actctgatac acatattcaa
    25981 ttttcatcaa atcgcgattg gctttgctgt catgccaacg ctggaaaacg attttgccgc
    26041 caatcggcgt gcgttcgttc tggtcatgca actgatacgg tttgaaatcc agcgccgtta
    26101 acagagaggc aatgttggag tcgtgcccaa ccaacactgt aattttcggt gcgctggtgc
    26161 gatcggtgac cagagctttg tcgatataac tgaccagcgg tttcgcaaca ttgcgcgcca
    26221 cttccggtga ggtaaacagg ctgtcctggt agccgttttt aagcttcgac aacaccttcc
    26281 actgctggtc agatttgatt tctccccagg ccacctgatc catcggaaaa ccttcgtaat
    26341 attgcaaagt aaacgcatct accagcgagt tgccgacttt cagcggcccg gaaacacctg
    26401 gttcttgttg atacttggcg ctaaaggtat ttttgccatc caccagcgaa cactgttgtt
    26461 tctctttaca ggcaggggaa tctttatagt taacgatttt ttccagtagc tggtagctgt
    26521 cggtaagctg gagtttgctg agctctttct ccattgccgc caccgcctgt tcactgaatg
    26581 cggcggaatc atcggtgatc accgggttaa aggttgggtc catagtgccc attttttcct
    26641 ggtgatgcac aggaatatca caccccggga atgcgccggt aataaagaac tgtgcggtcg
    26701 caacggtacg ttgcagacta ttggcatagg cataaacggt gtacggcggc gggcattccc
    26761 ccgatttcac catcccctgc tctgccagcc attcacgcat gtaatggccc atatacactt
    26821 cgagcacgcc acctttggtg gtgagttgcc caccggggac gtcccattct ggccatttat
    26881 tcggcgtcga ctgctccagc acactgccat tgttcgccag cggcgcacgt aagttatggc
    26941 ggctcatcat gagcacttgc tgtagctgat agccttccgg tacggtttgt gcctgagcgt
    27001 ttgaagcgag taaaactatc cctgccacag ctgcggcgat tagcgttttg ttcattcctg
    27061 gcacctcttt tgttatcaat cactaaagag tgtgacagaa atatgacttt tttccgggaa
    27121 caggttcgca aaatggcttg ttgaacgcct gtttttctat atacatcttc aaaaacaggc
    27181 aaggtttgac gcaaactatg cctgattagc ccctgcctcg cgtacgccgc tgataattgc
    27241 acctgtggct aaacagaata tctgcggcgt taccaataca gagacaatat ttaccaataa
    27301 aaaagtgtga gactaataac aatggttttt agcatatgcc tttgccatta aattgcataa
    27361 cgatttaaat agtcgagttg cgcattcatt tccttatttt ttaacttgta agcctgagca
    27421 aaaagttcag gagggaggta aacatcataa tgattgccag tgtttattaa ataaccatac
    27481 tcaagatctt catctgttga aaaggattca ttccctaagg agattgatgc cacattacta
    27541 ttaacaataa ataatgagca aatgctatct gaaagagcag tgaggaaatt tttcacctca
    27601 ttaatcctct tctctgggtc ggtgtctaaa acaaggcgtg ccttgttatc attaaatata
    27661 aactcgggta aatgcagtat gcattttttg tcattcaagc aactatcaat aaattatttt
    27721 ttaaaaaaca gaaagttcca tgacataaga ctaccctccc cctgaatttt gttgtcagat
    27781 tgtagatcaa cgccaaataa cgtctttaca tcttccctga gattatagaa tgccgtatca
    27841 acaagagcct cgatcgtttg tttatcaaca attttaataa actcttcgta tacttgcttt
    27901 tgcgtcttat ggctgcccag cgcccatgaa tactcatgtt cttttaaaac cagtatggcc
    27961 cttaacagac aatagccatc gccatctact ggagcgtatt ttaatccata agttttatta
    28021 atttcactct gaataaaaga tataccagac aaatcaatac cagaatctaa atcagatatt
    28081 cccggcgtat ttatctgttc tggctggata ctcatatttt catcatctaa acaatactca
    28141 gaaaaaacat catcacacgc agtaatgttt tcaatatatt ccataaagcc ataccaataa
    28201 taacctttaa aatctgtgat agactctgaa cctctgcccg gcaaggacac tctaacaata
    28261 tcaacatttt gaacatattg tatagtatat gtaatacctc ctgaaacccc tgaataaatt
    28321 tgtgtatcat tagtacgcaa tgttctcata aataatgata gatcattatc attctgcatt
    28381 tcctcaaatt ctttttctag tgattcacat tccagacatg gagtatcctc ttcatttact
    28441 ttacgtctga gttcattgta taaccgcata aaacacacca gtgtgttttc aaaaagcgtg
    28501 attgaatatg tttgattttt ttcgtactca ttttttatat cgttccaggc ctgtttcaaa
    28561 taattatttg cagtgca
//
LOCUS       DAZFXT010000058        27503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.058, whole genome shotgun sequence.
ACCESSION   DAZFXT010000058 DAZFXT010000000
VERSION     DAZFXT010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.058"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..2213
                     /locus_tag="ACHKW0_002645"
                     /pseudo
     CDS             1..2213
                     /locus_tag="ACHKW0_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            2221..3549
                     /locus_tag="ACHKW0_002646"
     CDS             2221..3549
                     /locus_tag="ACHKW0_002646"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HFO7475455.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECPTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(3596..4921)
                     /gene="rimO"
                     /locus_tag="ACHKW0_002647"
     CDS             complement(3596..4921)
                     /gene="rimO"
                     /locus_tag="ACHKW0_002647"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HFO7475456.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            5134..5517
                     /gene="bssR"
                     /locus_tag="ACHKW0_002648"
     CDS             5134..5517
                     /gene="bssR"
                     /locus_tag="ACHKW0_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HFO7475457.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            5628..6743
                     /gene="yliI"
                     /locus_tag="ACHKW0_002649"
     CDS             5628..6743
                     /gene="yliI"
                     /locus_tag="ACHKW0_002649"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HFO7475458.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQVGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRSGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(6740..7366)
                     /gene="gstB"
                     /locus_tag="ACHKW0_002650"
     CDS             complement(6740..7366)
                     /gene="gstB"
                     /locus_tag="ACHKW0_002650"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HFO7475459.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            7613..8815
                     /gene="dacC"
                     /locus_tag="ACHKW0_002651"
     CDS             7613..8815
                     /gene="dacC"
                     /locus_tag="ACHKW0_002651"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HFO7475460.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            complement(8862..9620)
                     /gene="deoR"
                     /locus_tag="ACHKW0_002652"
     CDS             complement(8862..9620)
                     /gene="deoR"
                     /locus_tag="ACHKW0_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HFO7475461.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKMAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(9678..10274)
                     /gene="ybjG"
                     /locus_tag="ACHKW0_002653"
     CDS             complement(9678..10274)
                     /gene="ybjG"
                     /locus_tag="ACHKW0_002653"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HFO7475462.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            10559..11791
                     /gene="mdfA"
                     /locus_tag="ACHKW0_002654"
     CDS             10559..11791
                     /gene="mdfA"
                     /locus_tag="ACHKW0_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HFO7475463.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            complement(11832..12116)
                     /gene="ybjH"
                     /locus_tag="ACHKW0_002655"
     CDS             complement(11832..12116)
                     /gene="ybjH"
                     /locus_tag="ACHKW0_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbjH"
                     /protein_id="HFO7475464.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(12202..13017)
                     /gene="ybjI"
                     /locus_tag="ACHKW0_002656"
     CDS             complement(12202..13017)
                     /gene="ybjI"
                     /locus_tag="ACHKW0_002656"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HFO7475465.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(13017..14225)
                     /locus_tag="ACHKW0_002657"
     CDS             complement(13017..14225)
                     /locus_tag="ACHKW0_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7475466.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALVGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGVPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            14309..14845
                     /gene="rcdA"
                     /locus_tag="ACHKW0_002658"
     CDS             14309..14845
                     /gene="rcdA"
                     /locus_tag="ACHKW0_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HFO7475467.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAPGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(15020..16705)
                     /locus_tag="ACHKW0_002659"
     CDS             complement(15020..16705)
                     /locus_tag="ACHKW0_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HFO7475468.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            16975..17352
                     /locus_tag="ACHKW0_002660"
     CDS             16975..17352
                     /locus_tag="ACHKW0_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HFO7475469.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(17382..17639)
                     /gene="grxA"
                     /locus_tag="ACHKW0_002661"
     CDS             complement(17382..17639)
                     /gene="grxA"
                     /locus_tag="ACHKW0_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HFO7475470.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            17799..18086
                     /locus_tag="ACHKW0_002662"
     CDS             17799..18086
                     /locus_tag="ACHKW0_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjC family protein"
                     /protein_id="HFO7475471.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDTLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            18070..18792
                     /gene="nfsA"
                     /locus_tag="ACHKW0_002663"
     CDS             18070..18792
                     /gene="nfsA"
                     /locus_tag="ACHKW0_002663"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HFO7475472.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRQ
                     TIIKESRPFILDYLHKQGWATR"
     gene            18853..19755
                     /gene="rimK"
                     /locus_tag="ACHKW0_002664"
     CDS             18853..19755
                     /gene="rimK"
                     /locus_tag="ACHKW0_002664"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HFO7475473.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            19843..20319
                     /locus_tag="ACHKW0_002665"
     CDS             19843..20319
                     /locus_tag="ACHKW0_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HFO7475474.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            20671..21783
                     /gene="potF"
                     /locus_tag="ACHKW0_002666"
     CDS             20671..21783
                     /gene="potF"
                     /locus_tag="ACHKW0_002666"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HFO7475475.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKKKDEAYQFLNYLLRPDVVAHISDNVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            21878..23011
                     /gene="potG"
                     /locus_tag="ACHKW0_002667"
     CDS             21878..23011
                     /gene="potG"
                     /locus_tag="ACHKW0_002667"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HFO7475476.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIATRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            23021..23974
                     /gene="potH"
                     /locus_tag="ACHKW0_002668"
     CDS             23021..23974
                     /gene="potH"
                     /locus_tag="ACHKW0_002668"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HFO7475477.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            23971..24816
                     /gene="potI"
                     /locus_tag="ACHKW0_002669"
     CDS             23971..24816
                     /gene="potI"
                     /locus_tag="ACHKW0_002669"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HFO7475478.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            24876..25364
                     /locus_tag="ACHKW0_002670"
     CDS             24876..25364
                     /locus_tag="ACHKW0_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HFO7475479.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            25405..26532
                     /gene="rlmC"
                     /locus_tag="ACHKW0_002671"
     CDS             25405..26532
                     /gene="rlmC"
                     /locus_tag="ACHKW0_002671"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016070 - RNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HFO7475480.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(26707..27438)
                     /gene="artJ"
                     /locus_tag="ACHKW0_002672"
     CDS             complement(26707..27438)
                     /gene="artJ"
                     /locus_tag="ACHKW0_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtJ"
                     /protein_id="HFO7475481.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
BASE COUNT         6810 a         6575 c         7094 g         7024 t
ORIGIN      
        1 gctttgaact tattcagaaa aacgttggct atttctttaa agatattgaa cgttcggccc
       61 tgacattaaa ggactcactg tatttattaa aaaatacaga ggagattcaa cgcgccgtga
      121 ttctgaaaat ggaaatgatg ccatttttag actcggtggg actggtactt gatgataata
      181 aatattatct cttttcgcgg agggcgaatg ataaaatcgt tgtttatcat caggaacaag
      241 taaatggacc gcttgtcgac gagtcagggc gggttatttt tgccgatttt aacccatcga
      301 aacgaccgtg gtcggtggct tcagatgact ctaacaacag ctggaatccg gcatacaatt
      361 gctttgatcg tccgggtaaa aaatgtatct cttttacgct acgtatcaac ggcaaagatc
      421 acgatttgtt agcggtagat aaaattcatg tcgatttaaa ctgacggtat ctgaacgagt
      481 atcttgatca aatcagcgct aatgatgaag ttctattttt gaaacaaggt catgagatca
      541 ttgccaagaa tcaactcgct cgtgaaaaac tgattattta taatagcgaa ggtaattata
      601 atattattga ttctgtcgat acggagtata ttgcaaagac atcagcagtg ccaaataatg
      661 cattgttcga aatctatttt tattatcctg gcggtaattt attgaacgca tcagataaac
      721 ttttttatct accgtttgct ttcattatta tcgtgttgct ggtggtatat ttcatgacca
      781 ctcgtgtgtt ccgtcggcaa ttttctgaaa tgaccgagct ggttaatacg ctggcgtttt
      841 tgcccgactc aacggatcaa atcgaggctc tgaaaattcg cgaaggcgat gcgaaagaga
      901 ttatcagcat caaaaattcg atcgcggaaa tgaaagatgc cgaaattgaa cggtcaaata
      961 aattgctctc actgatctct tacgatcagg aaagcggttt tattaaaaat atggcgatta
     1021 ttgagtccaa caataatcag tatctggctg tggggatcat caaactgtgt ggtctggaag
     1081 ccgtggaagc ggtgtttggt gttgatgaac gcaataaaat cgtcaggaaa ttgtgtcagc
     1141 gaattgccga gaaatatgcg caatgctgcg atatcgtgac attcaatgcc gatctctatt
     1201 tacttctgtg tcgggaaaat gtacagacat ttacccgtaa aatagcgatg gtaaacgatt
     1261 ttgacagcag ctttggctac cgcaatctgc gcatccataa gtctgccatt tgtgaacctt
     1321 tgcaggggga aaacgcctgg agttacgcag aaaaactgaa actggcgatt tccagtatcc
     1381 gtgaccatat gttctccgag tttatttttt gtgatgacgc gaaactcaac gaaatagaag
     1441 agaatatctg gattgcgcgt aatattcgcc atgcaatgga aattggcgaa ctattcctcg
     1501 tctatcaacc gatcgttgat attaacaccc gcgccattct gggcgcggag gcgttgtgcc
     1561 gttgggtgtc tgcggagcgg gggatcattt caccgctgaa gttcattacc attgctgaag
     1621 atatcgggtt tatcaatgag ctgggttatc agattattaa aacggcaatg ggtgaattta
     1681 gacattttag tcagcgtgcg tcgctgaagg atgatttctt actgcatatt aatgtttcgc
     1741 cctggcagtt aaacgaaccg cactttcatg agcgttttac caccatcatg aaagaaaatg
     1801 gcctgaaggc gaacagcctc tgtgttgaga tcactgaaac cgtgatcgag cgaattaatg
     1861 aacattttta tctcaatatt gaacaactgc gtaaacaagg ggtacggata tcgattgatg
     1921 actttggcac cggtttgtca aacctgaaac gtttttatga aattaatcca gatagcataa
     1981 aagtggactc gcaatttacc ggcgatattt tcggtactgc gggaaaaatt gtgcgcatta
     2041 ttttcgatct ggcgcgctat aaccggatcc cggtgattgc ggaaggcgta gagagcgaag
     2101 acgttgcgcg cgaattaatc aaattaggat gtgttcaggc ccaagggtat ctgtaccaga
     2161 aacccatgcc attctccgcc tgggataaaa gtggaaaatt agtaaaagag tagtttacgt
     2221 atgtccagaa tcaataagtt cgtacttaca gtcagtctgc tgatttttat catgatttca
     2281 gcagttgcct gcgggatcta cactcaaatg gtaaaggaac gggtgtatag cctgaaacag
     2341 tccgttattg atactgcttt tgcggtggca aatattgctg aatatcggcg tagcgtggca
     2401 attgatctta tcaatacgct aaatcccacg gaggaacagc tgttggttgg cttacgcaca
     2461 gcttacgctg actcggtttc accctcttac ttgtacgatg tcggtcctta tctgatttcc
     2521 agtgacgaat gtattcaggt aaaggagttc gagaaaaatt attgtgcaga tattatgcag
     2581 gttgtgaagt atcgacatgt caaaaataca gggtttatct cttttgacgg taaaaccttc
     2641 gtctattacc tctatccggt aactcacaat cgtagtctga tatttttgct tggtctggag
     2701 cgtttttctt tactgtcaaa atcgctggcg atggacagcg agaacctgat gttctctctg
     2761 tttaagaacg gtaaaccggt gaccggtgat gaatataatg ctaaaaacgc catcttcacc
     2821 gtttcggaag cgatggagca cttcgcctat ttgccgaccg gattgtatgt atttgcgtat
     2881 aaaaaagatg tttatttgcg ggtttgtaca ttgattattt tctttgccgc attggtggca
     2941 gtgatatcgg gtgccagttg cctctatctg gtacgcagag tgattaatcg tggtattgtg
     3001 gagaaagaag ccatcattaa taaccatttt gaacgcgtac tggatggcgg gcttttcttt
     3061 tcggctgccg atgtcaaaaa actctacagt atgtataact cggcgttcct ggacgacctg
     3121 accaaagcaa tgggcagaaa atcctttgac gaagatttaa aagcgctgcc ggaaaaaggc
     3181 ggttatttgt gcctgtttga cgtcgataaa ttcaaaaaca ttaacgacac cttcggtcat
     3241 ttgctgggcg atgaagtgtt gatgaaagtg gtgaaaatcc ttaaatcaca gatcccggta
     3301 gataaaggta aagtctaccg cttcggcggt gacgaatttg cggtgattta tacgggtgga
     3361 acgctggaag agttgctatc gattctaaaa gaaatcgttc atttccaggt gggaagcatt
     3421 aatttaagta ccagtatcgg tgtagcacat tcaaatgaat gtcctaccgt cgaacgcttg
     3481 aaaatgctgg cggatgagcg gctgtataag agtaagaaaa acggcagggc acagattagc
     3541 tggcagtaat cattattcgc aggccggaca aatgattttg cccggcctga attaattaaa
     3601 cccggctacc ccacaaatcg tactcatcgg cgtgctcgac tttcacacgc aggatatcac
     3661 ccggcttaac gttggtttca ccattgagat aaaccgcgcc gtcgatttcc ggtgcatctg
     3721 ccatgctgcg accaattgcg ccttcttcgt ccacttcgtc gataatcacc agaatttcac
     3781 ggcccacttt ctcttgcagg cgctcggcgg aaatctgctg ctgcagctgc atgaaacggt
     3841 tccagcgttc ttctttcact tcttccggaa cctggtcagg cagggcattg gcgtctgcac
     3901 cttcaaccgg gctgtattta aagcagccaa cgcgatccag acgcgcttct ttcaggaagt
     3961 cgagtagcat ctggaaatct tcttctgtct cgccagggaa gccgacaata aaggttgagc
     4021 gtagggtcag ttccgggcag atttcgcgcc actgtttgat gcgcgccagt tggcgatcta
     4081 cagaacccgg acgcttcatc agtttgagaa tgcgcgggct ggcgtgctgc aacggaatgt
     4141 ccagatacgg caggattttg ccttctgcca tcagtgggat gacgtcgtcc acatgcggat
     4201 aagggtaaac gtagtgcaga cgtgtccaga tccccagttt cgataactgt tcgcacaggc
     4261 tgaccatgct ggtttttacc ggctcgccgt tgtggaagcc agtacgatgt ttaacatcaa
     4321 cgccataggc ggaagtatcc tgcgagatca ccagaatctc tttaacgccc gcatctacca
     4381 gacgtttcgc ttcacttaac acttcgccaa tcggacggct caccaggtcg ccgcgcatag
     4441 acggaataat gcagaaggtg cagcggtgat tacagccttc tgaaattttc agataggcat
     4501 aatgacgcgg tgtcagtttc acaccttgtt ctggcaccag gctcaggaat gggttgtgtt
     4561 tcggttttgg cacgtagtga tgaacgtgct ccagaacctg ctcatagcta tgaggcccgg
     4621 tgatttccag cactttcggg tggacttcgc ggatctgatc ttcttttgcc cccagacaac
     4681 cggtcacaat taccttgccg ttttcattca acgcttcacc aatggcttcc agtgattctt
     4741 gtaccgcgct gtcaataaag ccgcaggtgt tgacgatcac catgtccgca tcgtcatagc
     4801 tcggtaccac gtcataacct tcagtgcgta gttcggtgag aatacgctct gaatcgacaa
     4861 ggtttttcgg acagccaagg gaaacaaagc cgattttcgg ctggggagtt actttgctca
     4921 tagcttaaaa aatattcagt tacaggaaag gtcagggcag ggattctaca gagttctgga
     4981 taaaatttgt atcgcaatct cattcgctgg cggaggcgaa ggaaatgtaa attttgttaa
     5041 tttggcgtga agaattgatc ctggacagca ttttgctcaa aaaatagcca tactatttaa
     5101 ttgcaacaag gctggaaaga ggaggatcga agtatgttcg ttgacagaca gcgaatcgat
     5161 ctgctgaacc ggttgatcga cgcacgcgtt gacctcgccg catacgtgca actgaggaag
     5221 gcaaaaggat acatgtccgt cagcgaaagc aatcatctac gagataactt ttttaaactg
     5281 aatcgcgaac tgcacgataa atcgctgcgg ttgaatcttc atctggatca ggaagagtgg
     5341 agtgctcttc atcatgctga agaagcatta gcgacagccg cagtatgttt gatgagtggg
     5401 caccatgatt gcccgactgt tattaccgtc aacgccgata agcttgaaaa ttgtctgatg
     5461 agcttaacgc tgagtatcca gagcctgcag aagcacgcca tgcttgagaa ggcctgaaaa
     5521 ctaaggggga gaaagcgtct cccccttcat gtttaagttt ttgtaaaaat gaatttgtta
     5581 tctcctccac tgactacgct ttaagccaga gtcaatccgg aggcgttatg catcgacaat
     5641 cctttttcct tgtgcccctt atttgtcttt cttccgctct ctgggcggct cctgcaacgg
     5701 taaatgtcga agtactgcaa gacaaactcg accatccctg ggcactggcc tttttacccg
     5761 ataatcacgg tatgttaatc actctgcgcg gcggcgagtt gcgtcactgg caagtaggaa
     5821 aaggattatc tgcgccgctt tccggagttc cggacgtttg ggcgcacggg cagggcggcc
     5881 tgctggacgt ggttttagcg cctgattttg ctcagtctcg ccgcatctgg ttaagttatt
     5941 ccgaagttgg cgatgatggc aaagccggaa ctgctgtggg ttatggccgc ttaagtgatg
     6001 atctctcaaa agtgaccgac ttccgcaccg tcttccgcca gatgccaaaa ctgtctaccg
     6061 gcaaccattt tggcgggcgg ctggtattcg acggtaaagg ttatcttttt attgctctgg
     6121 gcgaaaacaa tcagcgcccg acggcgcagg atctggataa attacagggc aaactggtgc
     6181 gtctgaccga ccagggcgaa atcccggatg ataatccttt tataaaggaa tccggtgcgc
     6241 gcgccgagat ctggtcttat ggcattcgta atccgcaagg aatggcgatg aatccgtgga
     6301 gtaatgcact gtggctgaat gaacatggcc cgcgcggtgg tgatgaaatt aatatcccgc
     6361 aaaaaggcaa aaactacggc tggccgctgg caacctgggg aatcaactat tcaggcttta
     6421 agataccgga agcgaaaggg gagatcgtcg ccgggaccga gcaacctgtt ttttactgga
     6481 aagattcgcc cgctgtgagc ggcatggcct tctataacag cgataaattc ccccagtggc
     6541 agcaaaaatt atttataggc gcgctgaaag ataaagatgt cattgtgatg agcgtcaacg
     6601 gcgacaaagt gacagaagat ggccgtattt taacggacag agggcagcga attcgtgatg
     6661 ttcgctctgg acccgacggt tatttatacg ttctcaccga cgagtccagt ggggaattac
     6721 ttaaagttag cccacgcaat tagctaacgg gaatcatcac cactttgcgc actgcggggc
     6781 gttcagtgag ttgctgatac cagcgttgca gatttggacg cggtgtccag gtcaggccaa
     6841 cgttaaacaa attgtagatg aacggtgcga tagcgatatc gcccacacca aactcgtcgc
     6901 cagagaacca ttttactttt gccagttccg catcgagcag ggcaaacagg gcgtcgcact
     6961 ctttgcaact ggcatcaatg gcggcctgat cgcgctcttc gggtggtgtt ctgactaatc
     7021 ccatcaggat cccgcgatga gcattactga gtgtctggtt tgcccagtcc atccattttt
     7081 ccgcttccgc acgacgtgcc ggtgagtcga tccacaggcg tttttgcccg tactgtgccg
     7141 ccagatagcg gacaatggcg tttgattccc aaagaatgag atcactttcg tcgtcacgca
     7201 acaacggcac cagcccgtta gggttcatcg ccagaaaatc agcatcgtga tttatcccaa
     7261 actcacggcc cgcgagaatt tgctcataag gtagttccag ttcttcgagc gtcagcaata
     7321 ctttttttac gttagttgaa ttattccgac cccacagcgt aatcatactc accccttttc
     7381 cgacaggcag ccacaggctg aacaggactc cgatggtgaa gtaaagttaa cttttaagca
     7441 acagctggca aaaaaatgac accagaagca gcgaagcggc aggatattgc ataaacttta
     7501 aaaactttac caacttacgg tttctttaag tttgtgtgtg cgttattaat caccgaactt
     7561 atcatacggc gatataacgt attttttttg aatggatact cgggtggcat ttatgacgca
     7621 atactcctct ctccttcgtg gtcttgcagc gggttctgca tttttattcc tttttgcccc
     7681 aacggcattc gcggcggaac aaaccgttga agcgccgagc gtggatgcgc gtgcatggat
     7741 tttaatggat tacgccagcg gtaaagtgct ggcagaaggc aacgcggatg agaaactgga
     7801 tcccgcgagc ctgactaaaa tcatgaccag ctatgtggtt gggcaggcgc ttaaggccga
     7861 taagattaaa ctcaccgata tggtgacggt cggtaaagat gcctgggcga cgggaaatcc
     7921 ggcactgcgt ggttcatcgg taatgttcct caaaccgggc gatcaggttt cggtggcaga
     7981 cttgaacaaa ggtgtgatta tccagtccgg taatgacgcc tgtattgcgc tggccgatta
     8041 cgttgccggg agccaggagt catttattgg tttgatgaat ggttatgcca aaaaactggg
     8101 tctgaccaac actaccttcc agacggtgca cggtctggat gcgccggggc agttcagtac
     8161 cgcgcgcgat atggcattgc tgggtaaagc attgatccac gatgtgccgg aagagtacgc
     8221 cattcataaa gagaaagagt tcaccttcaa caaaattcgt cagcctaacc gtaaccgtct
     8281 gctgtggagc agcaatctga atgttgatgg catgaagaca ggaaccacgg caggcgcggg
     8341 atataatctg gttgcttcgg ctacccaggg cgatatgcgt ttaatctccg tagtgctggg
     8401 ggcgaaaact gaccgtatcc gttttaatga gtctgagaaa ttattgacct ggggtttccg
     8461 cttctttgaa accgtgacgc caattaaacc tgatgccacc tttgtgactc agcgcgtctg
     8521 gtttggtgat aagagcgaag tgaatcttgg ggcaggtgaa gcgggctcag tgaccatacc
     8581 gcgtgggcag ctgaaaaacc tgaaggcgag ctacacgtta acggaaccgc agcttaccgc
     8641 accgctgaaa aaaggtcagg ttgtcgggac cattgatttc cagcttaacg gtaaatccat
     8701 tgagcagcgt ccgctgatcg tgatggaaaa tgtggaagag ggcggattct ttggtcggat
     8761 gtgggatttc gtgatgatga aattccatca gtggttcggc agctggttct cttaatcttc
     8821 tgataaccgg atggcgcgaa acgtcatccg gttatacgtc attaatacat caacttaata
     8881 cggtgcgtct gcgcgtactt cacatactca tcttccgggc aacaatcact caccacaata
     8941 tcaaagcgtt tcaggtcacc catgcgcgcc ggacgcacct tgccaaattt actgtggtcg
     9001 acaaccagca catgcttttg cgccatcgac atggcccagt gttttaccgg caactcttca
     9061 agattaaaac aggtagcgcc tttactgaca tgcacgcccg ccgcggaata aaaagcgata
     9121 tccgggcaaa aattattcaa cgtttgctga aaatcgatgg gtttgaaaat ggcgttgctg
     9181 gcgtgaaatt caccaccgca cagaaatgcg cggcaatggg gtttctcttt cagcgccaga
     9241 aaggtattta gcgaataaca aacggcggta aaagggattt cattatcaat cgcttcaata
     9301 atccacggcg tggtggtgcc acagtcaaaa aagagagtct gatcgggttc taccagtgtc
     9361 gcagccattt ttgccgcccg gcgtttttct tccaccaggc gggatttttg atcgcttaac
     9421 aggtaatggc tggcactgcg cggttccaga acaatatagc cgccgagcaa aacgacgggc
     9481 gcactgtggt tgttcagatc gcgacgaatc gtcatctccg aaaccccaag cagggcggcg
     9541 gcgtctttaa gatgtaactt atcgctgcgt tttaattctt gcagcagctg cccgatacgc
     9601 tcttcgcgac gtgtttccat aatccctctg aatagttatt gaagcgagcc gctcaatact
     9661 acacttttta gcagagatca gtcacgcacc cagcctttgc ggatcggtaa tgcaaaacag
     9721 aagcgatacc acgattgcag acgttgatag agtttatgcc ccatcgcttg ccagataatc
     9781 tgagcgctaa ggcagccaat catacctgcc agcaatccac cgagcatatc cagcggccag
     9841 tgcacgccaa gataaacgcg cgaccaggca atgacgacgg ccagcaccat taaaagtgag
     9901 ccggaccaca ggcgatgcca gcataaaaat gccagtgcaa aggtgaaaat caccgtaccg
     9961 tggtcgcttg ggaatgagtc atccgccgca tgatgcagga agttatagcc gatattttcg
    10021 acaaagggtc ggtcgtgcgg aaaaagatgt cccatcgtcc aggacacaaa caggctgacc
    10081 gccagcgcga tagcgatttt tatcaccagt tgccgttgtg ctgtaagccc ccacaaccaa
    10141 agcaccgcgg ccagcagcgg taccacggta atcaaatctt tagcaataaa aatcgccaac
    10201 gagatcatcc acggagccga gtctggcgtc gcgttaataa gagagaatag agagagattc
    10261 aaattttcca gcataacttc ccgacgcaaa gtgattaaaa gggagccaat acaggcaagt
    10321 cgttgagaat aaagtgcagg ttaaactggg taaagcggca tcgtcttatt tccctcaagc
    10381 ggcctgttta cggtgggtaa ttgtaacggg cataggttaa ataaaactta aagaaagcgt
    10441 agctatactc gcaataatgt aagaatgtgc ttaaccgtgg tttcagctac aaaattcgct
    10501 ttctcgttag ctgtgctttt attaaactct gcgcgattat tattggcgaa gaaattgcat
    10561 gcaaaataaa ttagcttccg gtgccaggct tggacgtcag gcgttacttt tccctctctg
    10621 tctggtgctt tacgaatttt caacctatat cggcaacgat atgattcaac ccggtatgtt
    10681 ggccgtggtg gaacaatatc aggcgggcat tgattgggtt cctacttcaa tgaccgcata
    10741 tctggcgggc gggatgtttt tacaatggct cctggggccg ctgtcggatc gtattggtcg
    10801 ccgtccggtg atgctggcgg gggtggtgtg gtttatcatc acctgtctgg caatattact
    10861 ggcgcaaaac attgaacaat tcactctgtt gcgcttcttg cagggcataa gcctctgttt
    10921 cattggcgct gtgggatacg ccgcaattca ggaatccttc gaagaggcgg tttgtatcaa
    10981 gatcaccgcg ctgatggcga acgtggcgct gattgctccg ctacttggtc cgctggtggg
    11041 cgcggcgtgg atccatgtgc tgccctggga agggatgttt gtcttgtttg ccgcattggc
    11101 agcgatctcc tttttcggtc tgcaacaagc catgcctgaa accgccacgc gtataggcga
    11161 gaaactgtca ctgaaagaac tcggtcgtga ctataagctg gtgctgaaga acggccgctt
    11221 tgtggcgggg gcgctggcgc tgggattcgt tagcctgcca ttgctggcgt ggatcgccca
    11281 gtcgccgatt atcatcatta ccggcgagca gttgagcagc tatgaatatg gcttgctgca
    11341 agtgcctatt ttcggggcgt taattgcggg taacttgctg ttagcgcgtc tgacctcgcg
    11401 ccgcaccgta cgttcgctga ttattatggg cggctggccg attattattg gtctgttggt
    11461 cgctgctgcg gcaacggtta tctcatcgca cgcgtattta tggatgaccg ccgggttaag
    11521 tatttatgct ttcggcattg gtctggcgaa tgcgggactg gtgcgattaa ccctgtttgc
    11581 cagcgatatg agtaaaggta cggtttctgc ggcgatggga atgctgcaaa tgctgatctt
    11641 taccgtcggt attgaaatca gcaaacatgc ctggctgaac gggggcaacg gactgtttaa
    11701 tctcttcaac cttgtcaacg gaattttgtg gctgtcgctg atggttatct ttttaaaaga
    11761 taaacagatg ggaaattctc acgaagggta aaaaaatgcc tgattgcttt gtgcgatcag
    11821 gcattctcga attaatggtg atggtcgtca atctggtgtt cgataaccat cccttcacct
    11881 acgctggcaa gatggcgaac ataaggatgc gggcggtaag ccggagctgg cgcaggagcc
    11941 acatagacgg tttgcggcac agtcgcgacg ctgaccgctt gtggaacgct gactgaatca
    12001 ggaaccacaa ccactttgta tccactcggc acatcgacgg tgacactttg cgccatcgcc
    12061 acaccagtaa agcccattaa aagcgcgccc aacagtagac aatttttcat aaacatttcg
    12121 cctgaaataa tgcagcgggt taatttccgc gaattatgca gccttcattc aggtattgtg
    12181 ttgccgggcg tgctgccgtt tttattggtc aaatggcgct tcgtgtttaa gcactttatc
    12241 gatcacatcc agtacgcctt cacggttatt ggagcctgcc cggtattttg ccgctgcgac
    12301 gaccgcattg ccggcatttt ccattgcaaa actaaagcct gcctgacgca gcatctcaat
    12361 atcgttaccg ccatcgccaa agaccaccac ttcgctgtcg tctattcccc ataatttctg
    12421 cagttggcga aggccattgg ctttatgtac gccggggata atcagatcga tgctgccgtt
    12481 gccggtgtgg accggcacca taatatcgcc gatggcctca tgtaatgctt tttgtacttg
    12541 tggaatcagt tcatcggaaa gattcaggcc aaacttaaag aagatatcct ctaagttgtc
    12601 aaagttatcg acgtattcca gacggtgata atacatttcc gccaccgttt tcatggcatc
    12661 gtcatatttt ttgagcgtat aggcgctatt ttttccgcag gcaataattt ccacttccgg
    12721 gcgcgtcagc aaatgttcca cgacagtagt aaacgcatcc ttcgacagct cgccattaaa
    12781 aacatctttg ccttcgctca ctacccagcc gccgttttca gccacaaagg caatttcatt
    12841 agcaatttca gggaagaaag agatcaactg ataatattga ttcccgctgg cgactacaaa
    12901 gcgaattcct tgtgctttca tttgctgata ctgagccata aaccgctcac ggttataggt
    12961 tttttgatcg cttaagaaag taccatccat gtctaccgca attaatttaa tgctcatcaa
    13021 ctattctcca tcgccgtctg cgttttggta tcgggtttgg cgacggcttt cgcgacaata
    13081 gccgcgagaa taaccagcgc cagtacaacc agcattgcac tacgtaatcc ataatgttcg
    13141 ccgagatagc ccagcagcgg cggcccgacg aggaaagcca gataaccggt cgttgctacc
    13201 acactgacgc gggttggtgc atcggggccg gtatcgctgg cggcagaaat ggtcagcggg
    13261 aagcccagcg aggcacccag tccccagagt acaacagaca ccccagcgac ccaggcgcta
    13321 tcgacaaaaa taatcagccc aatacccaac gcccccatta gcgcactggc ccgaaccacg
    13381 gcaacgcgac tgtaacggtc gatgaaccaa ccgccagtga agcgtccaac ggtcatcccc
    13441 agggtaaaac cggcataaat cagcgagccg gaagtggggc taaaaccgtg accgtcaacc
    13501 attaataagg gtaaccagtc gttggcagaa ccttcggcaa aggccatcgc cagcaccaca
    13561 acaccaatca gcagcaactg gatatcgcga taaaaaggta cgcctttttc gccatgctgg
    13621 gtgccatcgg cagcattttt gcccgtaccg tcagggattg cctgaatggc gatatatata
    13681 ggcgcgatgc ctaccagcgc cgccaataaa atgtgcaccg ttgccggaac gccaaaggcc
    13741 gtcagtgcca tcccgacacc agcgcctgcc agcgtgccca ggctataaaa accgtgcatc
    13801 atcggcaaaa ccgttttatt catttctcgc tcaacggcgg caccttcaac gtttatcgcc
    13861 acttccgcag aaccaaaact tgccccaaag acgccgagac caacggcaaa gagcaggggc
    13921 gatgtcagcc agagtgccag acttaatatc atcatcccga ccaatgcgca ggacatggtg
    13981 accaggatga cattacgtgt cccaaagcgt ttcactaacc acgccgagca gagaataccg
    14041 ctcatcgaac cgatcgacag accaaagaga acaccgccca tttcagcgat cgagacagag
    14101 agaatatcgc ggatagcagg cgtacgggtt gcccaggacg ccattaacag gcctggcaaa
    14161 aagaagaaca taaacagcgc ccaggttcgg cgtttcaatg cattacgtga agaatttacg
    14221 gtcatagatc acgtcaaaat aagaagaggg aagacaacat tagcaaggtt gtgtacattt
    14281 gtacacaatt gcagagagag gaaatgacat gcgtcgcgct aacgatccgc aacggcgaga
    14341 aaaaattatc caggccacgc tggaggcggt gaaactttac ggaatacatg ctgttacgca
    14401 ccgcaaaatt gctacccttg ccggggtacc gttgggatcg atgacctact atttttcagg
    14461 aattgatgag ttgttgctgg aggcgttcag cagttttact gagatcatgt cccggcaata
    14521 tcaggcattt tttagcgatg ttagtgatgc tccaggcgca tgccaggcta tcaccgatat
    14581 gatctacagc tcacaggttg caacgccgga taatatggag ctgatgtacc agctctacgc
    14641 gctggctagc cgaaaaccgc tattaaaaac ggtaatgcaa aactggatgc agcgcagtca
    14701 gcaaacgctc gaacaatggt ttgaacccgg aaccgcccgc gcgcttgatg cgtttattga
    14761 ggggatgacg ctgcattttg tcaccgaccg taagccgcta tcgcgcgagg agattttgag
    14821 gatggttgag agggttgcag ggtagtagat aaattttaga taacaaaaaa cccaccaacc
    14881 ttgaaccgaa atggcggggt tgatgggctc cacaaaatgg ggacatcaaa gaaaagcagt
    14941 ggcactaatt aagactgatg ccctgcggaa aagttctgcg gttgtgcaaa aaaatttcat
    15001 tttcagggca acttcagttt tatcctaatc ctggccatac catgacgatg attgtccctg
    15061 ccagcgtcag caggacgttg gcgattgcat aggtgcccgc atagcccagc gccgggatgt
    15121 tactgcgagc tgtatcactg atgatctcca ttgctggcgc gcaggtgcgt gcgcccatca
    15181 ttgcgccgaa caacagtgcg cggttcattc gcaatacata agcaccgaac aagaaacaga
    15241 taaccacggg cacaagactg acaatcaatc cggcaatcaa catctgaccg ccaatcgcgc
    15301 ccagaccgtt attaataccg ctaccggcgc tcagaccaac gcctgccata aacaccatta
    15361 agccgaactc tttcaccatg cttaatgcac cctgaggaat gtaaccgaag gtcgggtggt
    15421 tagcacgcat aaagcccagc ataattccgg cgaataacaa cccggcagcg ttccccatgc
    15481 cgaaactgaa tgtgctgaac tggaatgtga tcatcccgat catcagccca ataacaaaga
    15541 aggcgcagaa tgccagcaag tcagtgacct ggctgtgaat cgagataaag ccgatgcgat
    15601 cggcgatggt ttttacgcgg cgggcatcgc cgctgacttg taaaacgtca cctttgttaa
    15661 gcacgacgtt gtcatctatc ggcatctcaa tctggctacg aatgacgcgg ttaaggaagc
    15721 aaccgtgatc ggtcaacttc agttgtgcga gacgtttgcc aacggcgtta tggtttttaa
    15781 cgaccacttc ttcagtgacg atacgcatgt cgagaaggtc acgatcgaaa acttctttgc
    15841 cgttacggaa gctgggatcg agtcgggcat gggcgtcggg atagcctacc aacgctattt
    15901 catcgcccat ttgtagcacg gcatcaccgt ctggatttgc cagaatcccg ttacgtcgaa
    15961 tacgttcaat gtagcagccg gtttgtcgat aaatacccag ttcacgcaga tttttgccgt
    16021 cggtccaggc caccagctcc gggccgacgc gataggcgcg gatcaccggt aaataaacct
    16081 tacggttggc atcagtgtcc aggccacgtt cgcgggcgat ttgctgggcg ctggtctgta
    16141 agtcctgatg ctgcaatttc ggcaagtaac gcgcaccaac aatcaaactc accagaccga
    16201 ttaaataggt taaggcatac ccgaggctca gattatccag tgccagtgag agctgcctgc
    16261 tttccatgcc ggaatgacgc agtgtatcgc cagcaccgac cagaaccggt gtcgacgtca
    16321 tagagcctgc taacataccg gccgtcaggc caatatccca gccaaacagc ttacctaacc
    16381 ctaaggcgat caccagcgca ctgccaacca tcaccagtgc taacattagg taatttttcc
    16441 catcgcgaaa aaaaatggaa aaaaagttcg gtccggcttc gaccccgacg cagaaaataa
    16501 acagcataaa gccaagatta agcgcatcgg tgttaatgct gaaatgttgt tggcctaata
    16561 acagtgatac gactaaaacg ccaatggaat tacccagttg gatcgaacca agtcgtaact
    16621 ttccgagaca tagcccaagc gcgaggacca caaataataa cagaatgtaa ttcccattta
    16681 acaattcggc gacgtttata ttcacggagg ctaacttctt gtttactagt aagctgttga
    16741 aagaaatggt aatttacgat aatgtttttt accagaattc agggcgcaga ttcattcagc
    16801 gcacctaaac gatagtaaag taacaatata ttttactagt gtaatcacat taggtatcaa
    16861 cggctatatg aattgcgttg gcctatatta gcatggaatg cgaagcggct ttatcttact
    16921 gaacgccaca ctggcgaaaa atgtgttcga tagacgcagt gtcaggagga acgagtgaaa
    16981 cataaacaac gttgggcggg ggcaatctgc tgttttgtcc tcttcattgt ggtgtgcctt
    17041 tttctggcga cgcacatgaa aggcgctttt cgggctgccg ggcatcctga aatcggcttg
    17101 ctatttttca ttcttcctgg agcagtcgcc agcttctttt cacagcgtag agaagtcctg
    17161 aaacctctgt ttggcgcaat gctggcggca ccctgttcaa tgcttattat gcggctgttt
    17221 ttttcaccga cccgctcatt ctggcaagag ctggcatggt tactaagcgc ggtgttctgg
    17281 tgtgcgctgg gggcactgtg tttcttattt atcagtagtt tgtttaaacc acagcacaga
    17341 aaaaatcagt aaagccctca acgcgagggc ttgtcagacg atcaggcgtc cagattttct
    17401 ttcacccatg cagcaaaatc ggtatagccg ccgatatgtt gctgatcgac aaaaatctgc
    17461 ggcacggttt ctacgggttt acctgccttt tgttgtagat cttctttagt gatcccttcc
    17521 gcacgaatat ctacatactg atactgaaaa tcatcgcgtt cattgctcaa tttctcagcc
    17581 agatcttttg cacgcacaca gtaagggcaa cccggacgac caaaaataac ggtttgcatt
    17641 atttctctcc tcatagattt atgcctgtaa tgatcacgct aaaatgtatt cgctgaaagt
    17701 aggtttaacc tgttgcatta attgctaaaa gctataactg ttaaacacaa tacagtgaaa
    17761 agttttagac tgaaggctca ctttgcagag ggaagcgtat gcgcgcgatc ggtaaattgc
    17821 ctaaaagcgt gttgatactg gaatttatcg gaatgatgtt gctggcggtg gcgctgctgt
    17881 cggtaagcga caccctgtcg ctgcctgagc cattttctcg gccagaagtg cagattctga
    17941 tgatttttct cggtgttttg ctcatgcttc ccgctgcggt ggtggttatt cttcaggtgg
    18001 caaaacgtct tgccccacag ctgatgaacc gtccaccgca atattcacgt tcagaaagag
    18061 aaaaagataa tgacgccaac cattgaactt atttgtggtc atcgctccat tcgccatttc
    18121 actgatgaac ccatttccga agcgcagcgt gaggcgatta ttaacagcgc ccgtgcgacg
    18181 tccagttcca gttttttgca gtgcagtagc attattcgca ttactgacaa agcgttacgt
    18241 gaagagctgg tgacgctgac cggcgggcaa aaacacgtag cgcaagcggc ggagttctgg
    18301 gtgttctgtg ccgactttaa ccgccattta cagatctgtc cggatgctca gctcggcctg
    18361 gcggaacaac tgttgctcgg tgtcgttgat acggcaatga tggcgcagaa tgcattaacc
    18421 gcagcggaat cgctgggatt gggtggggta tatatcggcg gcctgcgcaa taatattgaa
    18481 gcggtgacga aactgcttaa attaccgcag catgttctgc cgctgtttgg gctgtgcctt
    18541 ggctggcctg cggataatcc ggatcttaag ccgcgtttac cggcctccat tttggtgcat
    18601 gaaaacagct atcaaccgct ggataaaggc gcactggcgc agtatgacga gcaactggcg
    18661 gaatattacc tcacccgtgg cagcaataat cgccgggata cctggagcga tcatatccgc
    18721 caaacaatca ttaaagaaag ccgcccattt attctggatt atttgcacaa acagggttgg
    18781 gcgacgcgct aaaaccgcca cgtcgatgta tgatacgcgg gcttttgacc aggtctgaca
    18841 gagaggtgca gggtgaaaat tgccatattg tcccgggatg gaacgctcta ttcgtgtaag
    18901 cggctgcgtg aagccgctat acagcgcggt cacctggttg aaattcttga tccgctttct
    18961 tgctacatga acataaatcc tgcggcgtct tctattcact acaaaggccg caagttaccc
    19021 cattttgacg cagtgatccc gcgtattggc accgccatta ccttttatgg gacggcggca
    19081 ctgcgccagt tcgagatgct ggggagctat ccgctcaatg agtcggtcgc cattgcccgg
    19141 gcgcgtgaca aattgcgttc catgcaactg ctggcgcgtc agggcatcga cctgcctgtc
    19201 acgggcattg cgcattcgcc ggatgatacc agcgatttaa tcgacatggt cggtggtgcg
    19261 ccgctggtgg tcaagttggt tgaaggcacg cagggaattg gcgtcgtgct ggcggagacg
    19321 cgtcaggcgg cggaaagcgt gattgacgct ttccgcggtc tgaacgcgca tattctggtg
    19381 caggaatata tcaaagaggc gcaagggtgc gatatccgct gtctggttgt tggcgatgaa
    19441 gtggtcgctg cgattgaacg gcgggcgaaa gagggcgatt ttcgttccaa tttgcatcgt
    19501 ggcggcgcgg caagcgtcgc cagtatcaca ccacaggagc gtgaaatcgc gataaaagcc
    19561 gcgcgaacga tggcgctgga cgttgctggt gtggatattc tgcgtgctaa tcgcgggccg
    19621 ttggtgatgg aggtgaatgc gtcgccgggg ctggaaggaa tagaaaaaac caccggtatc
    19681 gacatcgcgg gtaaaatgat ccgctggatc gaacgccacg ctacgacaga atattgcctg
    19741 aaaacgggtg gttagtcgca atcacattac tgatcatggt tttgcctgcg ctttttgcgt
    19801 aagctgtgcc ggtcttttta tcgaaagagg ttgtacaaaa ttatgacatc gctggtcgtt
    19861 cctggtctgg atacgctgcg tcaatggctc gatgacctgg ggatgagttt ttttgaatgt
    19921 gataactgtc aggctctgca tctgccccat atgcagaatt tcgacggtgt ctttgatgcc
    19981 aaaatcgatc tgatcgataa cacgatcctg ttttctgcca tggcggaagt ccgaccttca
    20041 gccgtattgc cgctggcggc ggatttatct gccatcaatg ccagttcgct gaccgtgaaa
    20101 gcatttcttg atatgcagga tgataatctg ccaaagctgg tggtttgcca gtctttatcc
    20161 gttatgcagg gcgtaaccta tgagcagttt gcatggttcg tgcgtcagag cgaagagcag
    20221 atttcgatgg tcattcttga agctaatgcc catcaactgc tgttaccgac tgacgatgaa
    20281 gggcaaaaca acgttaccga aaactatttt ctccactgat aattcctttc gagcacgcag
    20341 tcgctgccgc agtgactgcg cgctgcaaaa ttatctgctg tttttaacct tttcttaaag
    20401 attatttcac ttatcttgtg tcgatttggc tttatcacat agagcaaata cgcataaaaa
    20461 tttgttaaat accgtttttt aatccgagct atagtctcaa accctggcta aagttattct
    20521 tgcgatgctt ttatatagcg agcagtgctg gccgggagag agttctcttt tcttacaccg
    20581 cgccgataaa aaaatatgca cgtttattgc atatctttca gtgtgacaac ttttgttcgt
    20641 ttgttaacga actttcagaa ggaaagagat atgaccgcct taaataaaaa atggctatcg
    20701 ggtctggttg cgggtgctct gatggccgtc tctgtcggca cgctcgcggc tgaacaaaaa
    20761 acactccaca tttataactg gtctgattat atcgcccctg acacggtggc caattttgaa
    20821 aaagaaaccg gtattaaagt cgtctacgat gttttcgact ctaacgaagt actggaaggc
    20881 aaattaatgg cgggtagcac cggctttgac ttggtggttc catctgccag ctttcttgaa
    20941 cgccagctga cagcgggagt tttccagccg ctggacaaaa gcaaattgcc ggagtggaag
    21001 aatctcgatc cggagctgct gaagttggtc gccaaacacg atcccgacaa taaatttgct
    21061 atgccctata tgtgggcgac gaccgggatt ggctataacg ttgataaagt taaagctgtg
    21121 ctgggcgaaa acgcgcccgt cgatagctgg gacttgatcc tcaagcctga aaatctggaa
    21181 aaactgaaaa gctgcggtgt ctctttcctg gatgcgccag aagaagtttt tgctaccgtg
    21241 ttgaattatc tcggcaaaga tcccaacagc actaaagcgg atgattacac cggaccggca
    21301 accgatctgc tgttaaagct gcgcccgaac attcgttatt tccattcatc tcaatacatt
    21361 aacgacctgg caaacggcga tatttgcgtc gctatcggct gggcaggtga tgtctggcag
    21421 gcgtcaaacc gcgcgaagga agcgaagaat ggagtgaatg tctcgttctc gattccaaaa
    21481 gaaggggcga tggcgttctt tgatgtattc gccatgcctg cggatgccaa aaaaaaagac
    21541 gaagcctatc agttcctgaa ttacctgctg cgcccggatg tagtagcgca tatttccgac
    21601 aatgtgttct atgccaacgc caataaagca gccacgccgc tggtgagtgc ggaagtccgt
    21661 gagaacccgg gtatttatcc gcctgcggat gttcgtgcga agctgttcac tctgaaagtg
    21721 caggacccga aaatcgaccg tgtgcgcacc cgcgcgtgga ccaaagtgaa gagcggaaaa
    21781 taatccgcag ccttagatgc cgggcgggcg caccacaccc gccggcaatt cgcaccatca
    21841 tggtgcgctt gcacacattc aatgccggag agcagccgtg aatgacgcta tccctcgccc
    21901 gcaggcgaaa acccgtaagg cgctgacgcc gctattagaa atccgcaacc tcaccaaatc
    21961 ctacgatggt caacatgcgg tggatgatgt cagcctgacc atctacaaag gtgaaatctt
    22021 cgcgctgctg ggcgcatccg gctgtggcaa gtccacgctg ctgcgtatgc tggcaggttt
    22081 cgaacaacct tctgccggac agataatgct tgatggcgtc gatttgtcac aggtgccgcc
    22141 ttacctgcgc cccatcaata tgatgtttca gtcttacgcg ctgtttcccc atatgactgt
    22201 ggagcagaac atcgcctttg gcctgaaaca ggacaaacta ccgaaagcgg aaattgccac
    22261 ccgggtcaac gagatgcttg ggctggtgca catgcaggag ttcgccaaac gcaaaccgca
    22321 tcagctttcc ggtggtcagc gacagcgtgt ggccctggcc cgaagccttg ccaaacgccc
    22381 gaaactatta ctgctcgatg agccgatggg cgcgctggat aaaaagctgc gtgaccggat
    22441 gcagcttgaa gtggtggata ttctggagcg cgtcggtgtg acttgtgtga tggtcaccca
    22501 cgatcaggaa gaggcgatga ccatggcggg gcgtatcgcc attatgaatc gcgggaaatt
    22561 tgtccagatt ggcgagccgg aagagatcta cgagcatcca actacccgct acagcgctga
    22621 atttatcggt tcggttaatg tctttgaagg tgtactcaaa gagcgtcagg aagatggcct
    22681 ggtgcttgat tcgccggggc tggtgcatcc actgaaagtc gatgcggatg cttcagtggt
    22741 cgataacgtg ccggtacacg tagcgctgcg cccggaaaaa atcatgcttt gcgaagagcc
    22801 gcccgccaat ggttgtaact tcgcggtggg ggaggtgata cacattgcct atctcggcga
    22861 tctttcggtg tatcacgttc gtctgaaaag tgggcagatg attagcgccc agctacaaaa
    22921 cgcccatcgt catcgtaaag ggttaccgac ctggggcgac gaagtgcgtt tgtgctggga
    22981 agtggacagc tgtgtggtgc tgacggttta aggagcaaag atgaatacac ttgaacctgc
    23041 tgcccagtcg aaaccgccgg gcggatttaa gctgtggttg tcgcagctgc aaatgaagca
    23101 tgggcgcaaa ctggtcattg cattgccata tatctggttg atcttgctgt ttctgctgcc
    23161 atttctgatt gtctttaaaa taagcctggc ggagatggcg cgcgctattc caccttatac
    23221 cgaactgatg gagtgggctg acgggcaact gtccatcact cttaatctcg gtaattttct
    23281 gcaactgacc gacgatccgc tctatttcga tgcttatctc cagtcgctac aggtggctgc
    23341 gatttcgaca ttttgctgtt tactgatcgg ctatccgctg gcgtgggcgg tggcgcacag
    23401 taagccttcg acccgtaata ttttattact actggtgatc ctgccgtcgt ggacctcgtt
    23461 tctgatccgc gtttatgcct ggatgggaat attaaaaaac aacggtgtgc tgaataattt
    23521 tctgctgtgg ctgggggtta tagaccagcc gctgaccatt ctgcatacca atctggccgt
    23581 ttatatcggc attgtttacg cttacgtgcc gtttatggta ctgccgattt ataccgcgtt
    23641 gattcgtatt gattattcgc tggtggaagc agcgctggat ctcggtgcac gaccgctgaa
    23701 aacgttcttt actgtgatcg tgccgctgac taaaggtggg attattgccg gatcgatgct
    23761 ggtgtttatc ccggctgtgg gcgagtttgt gatcccggaa ctgctcggtg gcccggacag
    23821 catcatgatc gggcgcgtgc tatggcaaga gtttttcaat aaccgcgact ggccggtggc
    23881 ctcggcggta gcgatcatca tgttgctgct gctaattgtg ccgataatgt ggttccacaa
    23941 acaccagcaa aaaagcgtgg gagaacacgg atgaataatt taccggtagt tcgttcaccc
    24001 tggcggattg tgattttgct gctgggcttt acctttctct acgcgccaat gctgatgctg
    24061 gttatctatt cgtttaacag ctcgaagctg gtgacggtgt gggccggctg gtcaacgcgc
    24121 tggtatggtg agttattgcg cgatgacgcg atgatgagtg cggttggttt aagcctgaca
    24181 attgctgcct gtgcggcaac ggcggcggcg atcctcggga ctattgcggc ggtggtgctg
    24241 gtgcgctttg gcaggtttcg cggatcaaat ggctttgcct ttatgatcac cgcgccgctg
    24301 gtgatgccag atgtcatcac gggcttgtcg ctgttgttat tattcgtcgc gcttgctcat
    24361 gccattggct ggcctgcgga ccgcggtatg ctcaccatct ggctggcgca tgtcactttt
    24421 tgtacggctt atgtggcggt cgttatttct tcaagattgc gggaactgga tcgctcgata
    24481 gaagaagcag cgatggatct cggtgcgacg ccgctgaaag tgtttttcgt cattacgcta
    24541 ccgatgatca tgcccgcgat catttctggc tggttactgg cttttacttt gtcgcttgat
    24601 gatctggtga tcgccagctt tgtttccggg ccgggagcca ccacgttacc gatgctggtc
    24661 ttttccagcg tgcggatggg ggtgaatccg gaaatcaacg cccttgcaac gttaattctc
    24721 ggtgcggtcg gaattgtcgg atttatcgcc tggtatctga tggctcgcgc agaaaaacag
    24781 cggatacgcg atatccagcg tgcaagacat ggctgaagac actaaaattt gccaacctgg
    24841 ctacataatg ccgcgcatgc cgcggcattg ttttcatgga agacgaaacg ttgggatttt
    24901 ttaagaaaac atcttcatct catgctcgcc tgaatgtgcc tgcgctggtg caggtggcgg
    24961 cgctcgccat tattatgatc cgtggcctcg acgtgctgat gattttcaat acgctgggcg
    25021 tgcgcggtat tggcgagttc attcatcgca gcgtacaaac ctggagttta acgctggtct
    25081 ttttaagcag tctggtgctg gttttcattg agatctggtg tgcgttttca ctggtgaaag
    25141 ggcgtcgctg ggcgcgctgg ctatatctgc tgacacaaat caccgccgca agttacttgt
    25201 gggcggcttc gctggggtac ggttatccgg agctgttcag cattcccggt gaatcaaaac
    25261 gtgaaatctt ccatagcctg atgctgcaga agctgccgga tatgctcatc ctgatgctgc
    25321 tgttcgttcc ctcgaccagt cggcggttct tccagttgca ataatgtgta taatcgtcgc
    25381 ccctgatgat gtgaaggtca atgtatgcag tgcgcacttt acgacgcggg tcgctgtcgt
    25441 tcctgtcagt ggataacgca gccgattcca gagcaactct ccgctaaaac cgccgatctt
    25501 aaaaatctgc tcgccgattt tccggttgag gaatggtgcg cgccggtgtc aggcccggaa
    25561 caagggtttc gtaataaagc caaaatggtg gtgagtggta gcgttgaaaa accactgctc
    25621 ggtatgctgc atcgcgacgg cacaccagaa gacctttgtg actgcccgct ttatcctgcc
    25681 tcatttgcgc ccgtttttgc ggcgctaaaa ccgtttatcg cccgtgcggg gctgacaccc
    25741 tacaacgtgg cgcgtaagcg tggcgaactg aaatacattc tgctgactga aagccagagc
    25801 gatggcggca tgatgctgcg ctttgtactg cgttctgaaa ccaaactggc acaactgcgt
    25861 aaggcgctgc cgtggttaca ggaacaacta ccgcaactga aagttattac cgtcaatatt
    25921 cagccggtac atatggcgat tatggaaggg gagacggaga tctaccttac tgaacaacag
    25981 gcgctggcag agcgttttaa tgacgtaccg ctgtggatcc gtccgcaaag tttcttccag
    26041 actaatccgg cggtcgccag ccagctgtac gctaccgcgc gcgactgggt acggcagctg
    26101 ccggttaaac atatgtggga tctgttctgc ggtgtgggtg gctttggttt acactgcgcg
    26161 acgcctgaca tgcagttaac cgggatcgaa attgcatcag aggccattgc ctgtgcaaag
    26221 cagtcagccg ctgaactggg cttaacgcgt ttgcaatttc aggcgctgga ctccactcag
    26281 tttgccaccg ctcaggggga ggtgccggag ctggtgctgg ttaatccgcc gcgccgcggc
    26341 attggtaaac cgctatgtga ttatctctca acaatggcac cgcgttttat tatctactcc
    26401 agctgtaacg cccaaaccat ggcgaaagat atccgcgaac tgccaggtta ccgcatcgaa
    26461 cgggtacagc tttttgatat gttcccgcac accgcgcact atgaagtgct gacgctgctg
    26521 gtgaagcaat aaaaaagccg caggtgcggc ttcagattgc tgatcaggtg cgctttgttc
    26581 atgccggatg cggcgtgtac gccttatccg gcctacaaaa tcatgctaaa tcaatacatt
    26641 gcagagttga tgtgggccgg ataagcgcag cgcatcaggc aatcttactt ttgtcatcag
    26701 tctcaattac tgcggaaacc actggtcatt gattttctga tatgtaccgt cagctttaat
    26761 tgctgccagc gcgttattca gtttttccag cagagctttg ttatccggac gtacagcgat
    26821 gcccagaccg gtgccgaagt attgcggatc ggtcactttt tcagtagcaa cgcccagttg
    26881 cggattggtt ttcagccatt cgtttaccac cgctgtgtca ccaaataccc catcaatacg
    26941 accatttttc agatcgataa aggcattctg ataactgtca taggagacag ttttcacttc
    27001 ggggtgctga tcctgaatat atttctggtg cgtggtgccg ttttccatcc caatacgttt
    27061 gcctttcaga tcggcaaacg ttttgtaggt atcttttttg gcaatcacga cggctgagtt
    27121 ttcatagtag ggcgtggtaa acgatacctg cttgctacgc tcgggcgtga tatccatacc
    27181 ggagattacg gcgtcatatt ttctgaattt cagtgacggg atcaggctgt cgaacgcgtg
    27241 attagtaaaa gtacattctg cctgcatttg tttgcacaag gcttttgcca gatcgatatc
    27301 aaagccgaca atctcattat tagcacctat agattcaaag ggtggatagg tggctgaaac
    27361 gccaaaattg attttctctg cggcagaagc accgaaagta aaggaagcaa gtaaagcggc
    27421 aagaactaac tttttcatga tggaactccc gtctgtcaat cttatgattt ttggccgtgt
    27481 ctgcggcatg ggataacaat gcc
//
LOCUS       DAZFXT010000059        26599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.059, whole genome shotgun sequence.
ACCESSION   DAZFXT010000059 DAZFXT010000000
VERSION     DAZFXT010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.059"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..111
                     /locus_tag="ACHKW0_002673"
     CDS             <1..111
                     /locus_tag="ACHKW0_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7475482.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSAIWQKLIPNQADF"
     gene            176..424
                     /locus_tag="ACHKW0_002674"
     CDS             176..424
                     /locus_tag="ACHKW0_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7475483.1"
                     /translation="MARLILLTEWAKEEFSEPVPTPSTLSKYAKAGMIFPLPKKVGRR
                     WRVDPQARFVGMVNKPEVIATDHPALKRILEDGAPAKI"
     gene            402..1532
                     /locus_tag="ACHKW0_002675"
     CDS             402..1532
                     /locus_tag="ACHKW0_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO7475484.1"
                     /translation="MARPRKYKTDVPGLSPYFDKRNNKVYWRYRHPITGKNHGLGSID
                     QKLAETIAAEANSRLARQQMEQMLSLQEKIISDTGGSSTVTIFLNNYRKIQQERYENG
                     EIKLNTLKQKAAPLRVFDERFGTRPLDAITVKDVVSVLEEYKARGHNRMGQIFRKVLI
                     DVFREAQQTGDVPPGFNPAESAKKPQVRISRQRLTFDEWMMIYNAAEKDGYFLQRGML
                     LALMTGQRLSDICKMQFSDIRDGYLHVEQQKTGTRIAIPLALRCDKLNLTLDDVVSSC
                     RDCVLSPWLLHHHHAKGTAKRGGMVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQR
                     SLSERLFREQGVDTKILLGHSNQKMTDIYNDARGKEWKKLVI"
     gene            complement(1578..2216)
                     /gene="ompW"
                     /locus_tag="ACHKW0_002676"
     CDS             complement(1578..2216)
                     /gene="ompW"
                     /locus_tag="ACHKW0_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HFO7475485.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            2456..2545
                     /locus_tag="ACHKW0_002677"
                     /pseudo
     CDS             2456..2545
                     /locus_tag="ACHKW0_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            2573..3316
                     /gene="yciC"
                     /locus_tag="ACHKW0_002678"
     CDS             2573..3316
                     /gene="yciC"
                     /locus_tag="ACHKW0_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciC family protein"
                     /protein_id="HFO7475486.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            3346..3885
                     /gene="yciB"
                     /locus_tag="ACHKW0_002679"
     CDS             3346..3885
                     /gene="yciB"
                     /locus_tag="ACHKW0_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HFO7475487.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3990..4388
                     /gene="yciA"
                     /locus_tag="ACHKW0_002680"
     CDS             3990..4388
                     /gene="yciA"
                     /locus_tag="ACHKW0_002680"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HFO7475488.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(4428..5147)
                     /gene="tonB"
                     /locus_tag="ACHKW0_002681"
     CDS             complement(4428..5147)
                     /gene="tonB"
                     /locus_tag="ACHKW0_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HFO7475489.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPAVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARLTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            5371..5667
                     /gene="yciI"
                     /locus_tag="ACHKW0_002682"
     CDS             5371..5667
                     /gene="yciI"
                     /locus_tag="ACHKW0_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HFO7475490.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            5967..7220
                     /gene="kch"
                     /locus_tag="ACHKW0_002683"
     CDS             5967..7220
                     /gene="kch"
                     /locus_tag="ACHKW0_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HFO7475491.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESAQK"
     gene            complement(7275..7448)
                     /gene="yciY"
                     /locus_tag="ACHKW0_002684"
     CDS             complement(7275..7448)
                     /gene="yciY"
                     /locus_tag="ACHKW0_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HFO7475492.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            7591..9051
                     /gene="cls"
                     /locus_tag="ACHKW0_002685"
     CDS             7591..9051
                     /gene="cls"
                     /locus_tag="ACHKW0_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008808 - cardiolipin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HFO7475493.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            9086..9415
                     /gene="yciU"
                     /locus_tag="ACHKW0_002686"
     CDS             9086..9415
                     /gene="yciU"
                     /locus_tag="ACHKW0_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HI1450 family dsDNA-mimic protein"
                     /protein_id="HFO7475494.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(9468..10472)
                     /gene="oppF"
                     /locus_tag="ACHKW0_002687"
     CDS             complement(9468..10472)
                     /gene="oppF"
                     /locus_tag="ACHKW0_002687"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppF"
                     /protein_id="HFO7475495.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(10469..11482)
                     /gene="oppD"
                     /locus_tag="ACHKW0_002688"
     CDS             complement(10469..11482)
                     /gene="oppD"
                     /locus_tag="ACHKW0_002688"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP-binding protein OppD"
                     /protein_id="HFO7475496.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(11494..12402)
                     /gene="oppC"
                     /locus_tag="ACHKW0_002689"
     CDS             complement(11494..12402)
                     /gene="oppC"
                     /locus_tag="ACHKW0_002689"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HFO7475497.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(12417..13337)
                     /gene="oppB"
                     /locus_tag="ACHKW0_002690"
     CDS             complement(12417..13337)
                     /gene="oppB"
                     /locus_tag="ACHKW0_002690"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HFO7475498.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(13423..15054)
                     /gene="oppA"
                     /locus_tag="ACHKW0_002691"
     CDS             complement(13423..15054)
                     /gene="oppA"
                     /locus_tag="ACHKW0_002691"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HFO7475499.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEARRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            15338..15634
                     /locus_tag="ACHKW0_002692"
     CDS             15338..15634
                     /locus_tag="ACHKW0_002692"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475500.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(15792..16439)
                     /gene="ychE"
                     /locus_tag="ACHKW0_002693"
     CDS             complement(15792..16439)
                     /gene="ychE"
                     /locus_tag="ACHKW0_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HFO7475501.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            complement(16470..16523)
                     /gene="ychE"
                     /locus_tag="ACHKW0_002694"
     CDS             complement(16470..16523)
                     /gene="ychE"
                     /locus_tag="ACHKW0_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YchT"
                     /protein_id="HFO7475502.1"
                     /translation="MLSKGVLSARLFNLIYG"
     gene            16916..19591
                     /gene="adhE"
                     /locus_tag="ACHKW0_002695"
                     /gene_synonym="adhC"
     CDS             16916..19591
                     /gene="adhE"
                     /locus_tag="ACHKW0_002695"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004022 - alcohol dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006066 - alcohol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HFO7475503.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            complement(19893..20510)
                     /gene="tdk"
                     /locus_tag="ACHKW0_002696"
     CDS             complement(19893..20510)
                     /gene="tdk"
                     /locus_tag="ACHKW0_002696"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HFO7475504.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            21115..21528
                     /gene="hns"
                     /locus_tag="ACHKW0_002697"
     CDS             21115..21528
                     /gene="hns"
                     /locus_tag="ACHKW0_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0071824 - protein-DNA complex organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone-like nucleoid-structuring protein H-NS"
                     /protein_id="HFO7475505.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(21672..22580)
                     /gene="galU"
                     /locus_tag="ACHKW0_002698"
     CDS             complement(21672..22580)
                     /gene="galU"
                     /locus_tag="ACHKW0_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HFO7475506.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(22782..23795)
                     /gene="rssB"
                     /locus_tag="ACHKW0_002699"
     CDS             complement(22782..23795)
                     /gene="rssB"
                     /locus_tag="ACHKW0_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HFO7475507.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(23887..24792)
                     /gene="rssA"
                     /locus_tag="ACHKW0_002700"
     CDS             complement(23887..24792)
                     /gene="rssA"
                     /locus_tag="ACHKW0_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004622 - lysophospholipase activity
                     [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HFO7475508.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            24905..25363
                     /gene="ychJ"
                     /locus_tag="ACHKW0_002701"
     CDS             24905..25363
                     /gene="ychJ"
                     /locus_tag="ACHKW0_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HFO7475509.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            25413..26255
                     /gene="purU"
                     /locus_tag="ACHKW0_002702"
     CDS             25413..26255
                     /gene="purU"
                     /locus_tag="ACHKW0_002702"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HFO7475510.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            26415..26499
                     /locus_tag="ACHKW0_002703"
     tRNA            26415..26499
                     /locus_tag="ACHKW0_002703"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26449..26451,aa:Tyr,seq:gta)
     gene            complement(26481..26599)
                     /locus_tag="ACHKW0_002704"
                     /pseudo
     CDS             complement(26481..26599)
                     /locus_tag="ACHKW0_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518769.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7023 a         6489 c         6138 g         6949 t
ORIGIN      
        1 gctatcccat tcgaaggtga gcgccacaat gcgctggatg acgctcgtta ccaggcaaaa
       61 tacgtttcag ctatctggca aaaactgatc ccgaatcagg ctgattttta atgttcaacc
      121 ccggtcgttg cccaccagct atagtggcgg cgaccatgat tagcgaacga cgctcatggc
      181 aagacttatt ctgctcactg agtgggcaaa agaggaattc agtgaaccgg tccctactcc
      241 gagtacgtta agtaaatacg ctaaagccgg aatgatattt cctctcccca aaaaagttgg
      301 aagacgctgg cgagtggatc cacaagctcg ctttgtcgga atggtaaaca agccggaggt
      361 gatcgccaca gatcaccctg ctttgaagag gatactggaa gatggcgcgc ccgcgaaaat
      421 ataaaaccga tgttccggga ttatctccgt attttgacaa aagaaataac aaagtttact
      481 ggcgttacag gcatcccata acaggcaaaa atcacggtct cggcagtatt gaccagaaac
      541 tggcagaaac tattgcagca gaagcgaaca gccgtcttgc ccggcagcaa atggaacaaa
      601 tgctcagtct gcaggagaaa attattagtg ataccggcgg ttcatcaacc gttaccattt
      661 ttctgaataa ttacagaaaa attcaacagg aaagatatga aaacggcgag atcaaactca
      721 acacgctgaa acagaaagcg gcccctctca gggtatttga tgaacgtttt ggcaccagac
      781 cgttagatgc cataaccgta aaagatgtgg tatcagtact ggaagagtac aaggccagag
      841 gacataacag aatgggacaa attttcagga aagtactgat cgatgttttc cgggaagctc
      901 agcaaacggg cgatgtcccg ccaggcttta accctgcaga atctgcaaaa aaaccgcagg
      961 tgcggatatc aagacagcga ctgacttttg atgagtggat gatgatttat aacgcagcgg
     1021 aaaaggatgg ttacttttta cagcgcggta tgctgctggc actgatgaca ggccagcgcc
     1081 tttcagatat ttgcaaaatg caattttcgg atatccggga tggttatctt catgtcgaac
     1141 agcaaaaaac aggaacccgg attgccatcc ctctggctct gcgttgcgat aaattaaatc
     1201 tcaccctgga tgatgtggtg tcatcctgcc gcgattgcgt tcttagtccg tggctattgc
     1261 accaccatca cgcgaaaggg acagctaagc gcggcgggat ggttaagcca gcaacattaa
     1321 ccgttgcatt taaaaaagcc cgggattctg tggattacaa ctggcgtgct aatggcaccc
     1381 caccctcttt ccatgagcag agatctttat cagagcgatt gttcagagag cagggggttg
     1441 ataccaaaat tttgctaggc cattcgaatc aaaaaatgac cgatatttac aacgacgcac
     1501 gcggtaagga atggaaaaaa ctggtcattt gatgaccagt tttgcagagg ggttttgcag
     1561 aggttttgca gagaaaatta aaaacgatat cctgcggaga acataaacac ccacggatcc
     1621 agtcgtaccg agtctttcac ggtagtaaca ccagatttat acttagccgt ggtatcgata
     1681 tccatgtacc acactgacat gttaaccaac cagtcacggt taatcagata atcaaccccc
     1741 acctgcccgg cagctcccca ggaatctttc agactgagat cggaaagccc cgcctctttg
     1801 ccatgatcgt taaatccatt atcaaagaag gtggtgtagt taataccggc cccaacgtaa
     1861 ggacggaatt tgctgctggc atcaccaaaa taccactgcg ccatcagtgt tggcggcaga
     1921 tggtgaacgg ttgcaatatc gccggtcgcc cgggtgccga ttttatggcg gaacggcgtc
     1981 gctgccagta attccacacc aatgttgtcg gtcgccatat aagtaaacgt caagcccagt
     2041 tgagtgttat tggtcacgct gaatccaccc agacttccta acgtaccacc agcaccttct
     2101 gttggacgta cggttgcaga acctgcacgc ataaaaaatt cgcctgcttc atgcgcaaag
     2161 gcactgccag aaagaagagt tgttactgcc aaagccgcca ctgttaactt tttcatatcc
     2221 gctccgtcgt tatggttata aaaacgtgac gaatatacct acattggagt aacaaatgat
     2281 cctacataga tcacattaca tatgataatt caccatttat tgatctggat taatttagaa
     2341 gttgcattga gaatcctggt taatgtgatt taaatcaatt tttaaatatt ttctctacgc
     2401 aaatattaac tcgatcgcca cttgccgcgg tttgcccacg cctgaaggcc agcaattacc
     2461 tccgctttac aaagatatgc ttttccatac aagacctgat gctgccaccc gaggcttaac
     2521 cagggtacaa ttgcccgcta attaacacct gcataaaact caaggagagt gcatgtctat
     2581 cacggcgcag tccgtatacc gtgacaccgg aaatttcttc cgtaatcaat ttatgaccat
     2641 tctgttggta tcgttgctat gtgcgtttat cacagtggtg ttagggcatg ttttctcacc
     2701 cagtgatgca cagcttgcgc agctcaatga cggcgtgccc gttagcggca gtagtgggtt
     2761 gttcgacctg gttcagaata tgtcaccgga acagcaacaa attttgctgc aggcttcagc
     2821 ggcgtccact ttttcaggat taatcggtaa cgccattctc gccggaggcg taatattaat
     2881 tatccagctg gtgtctgcgg gtcagagagt cagtgcgctg cgagctattg gtgccagtgc
     2941 gccgatattg cccaagttat ttattctgat ttttctgact acccttttag tacagattgg
     3001 catcatgctg gtggtcgttc cgggaattat catggccatt ttactggccc tggcaccggt
     3061 gatgctggta caggacaaaa tgggcatttt tgcctcgatg cgtagcagta tgcggctgac
     3121 ttgggcgaat atgcgtctgg tggcacccgc agtactgagc tggttgctgg caaaaacact
     3181 gttgctgctt tttgcctctt cttttgccgc attaaccccg gaaattggtg ccgtactggc
     3241 gaacaccttg agcaacctga tttctgccat attgctcatc tatctgttcc gcctgtatat
     3301 gttgattcgc caataacctt aagcatttga ttacggaatc gtaaaatgaa gcagtttctt
     3361 gattttttac cgctggttgt ctttttcgcg ttttacaaga tttatgacat ctatgcggct
     3421 actgcggcgc tgatcgtcgc cacggcgatt gtgcttatct atagctgggt tcgctttcgt
     3481 aaggttgaga agatggccct gatcactttt gttctggtgg tcgtcttcgg tggcttgacg
     3541 ctgttcttcc acaatgatga gtttattaaa tggaaggtta cagtcattta tgctctgttt
     3601 gcgggagccc tgttagtcag ccaatgggtg atgaaaaagc cgctaattca gcggatgctg
     3661 ggtaaagaac tcacgctgcc gcaaccggta tggtcgaagc taaatctggc ttgggctgtt
     3721 ttctttatcc tttgcggtct ggcaaacatc tacatcgcat tctggctgcc gcaaaatatt
     3781 tgggtcaact ttaaagtctt tggcctgacc gcccttacct taatcttcac attgttaagc
     3841 ggtatctata tctaccgcca catgccgcag gaagataaat cctaaccaga ctgccagtcc
     3901 ttcggggctg gcgctgtatc tcctctgttc tcctcaggaa aatcatagta gcatcgcgcc
     3961 tgtgattttc cttttaagtc ggttttacca tgtctacaac acataacgtc cctcagggcg
     4021 atcttgtttt acgtacttta gccatgcccg ccgataccaa tgccaatggt gacatctttg
     4081 gtggttggtt aatgtcacaa atggatattg gcggcgctat tctggcgaaa gaaattgccc
     4141 acggtcgcgt agtgactgtg cgggttgaag gaatgacttt cttacggccg gttgcggtcg
     4201 gcgatgtggt gtgctgctat gcacgctgtg tccagaaagg gacgacatcg gtcagcatta
     4261 atattgaagt gtgggtgaaa aaagtagcgt ctgaaccaat cgggcaacgc tataaagcga
     4321 cagaagcatt atttaagtat gtcgcggttg atcctgaagg aaaacctcgc gccttacctg
     4381 ttgagtaata gtcaaaagcc tccggtcgga ggcttttgac tttctgctta ctgaatttcg
     4441 gtggtgccgt taattttaaa caggatattc accacaatcc cactgcctgg cttacccggc
     4501 tcataacgcc atctgcgcat cgcatttttc acctcacgct caaacatgtt cgcaggcttg
     4561 gctgagagga tttgtacgtt atccacgcga ccatccggcg tgacgtcaaa tttaacttta
     4621 acctgccctt caatgcgcaa tgcctgtgct cgtgccggat actgcggctg attacggctt
     4681 aatgcgcgtg gtcctgaagc cacactggta accggcttgc tggttgcagc cgttgccgta
     4741 cttgatgtca ggcgtgcggg tgccgtattt tcaaacggtg atgccggacg cgactctacg
     4801 ggtttgacat cgcgttttgg ctgctcctgt acctttttca ccggcttcgg ttttggctta
     4861 ggtttcggct tcggcttttc aatgaccgcc ggtgcttctt ttggtggttc ggggatcggc
     4921 tcaggttccg gttctggctc taccaccggc tccggtggcg gctgaacggc ttgtggcggt
     4981 tcgagatcag caggcgcaac catcgtgaca gaaattggct gcgcaggcgc aggtagttca
     5041 ataacctgat gtaccgaggt atagagcaga cccgccacaa cagcaccatg aatgcagacc
     5101 gaaagtaacg tcggccaggg gaagcggcga ggtaaatcaa gggtcattga agtcataatc
     5161 atttcagtag aaaaaccagg tctcgatttt aaatgcaaat agcaatcata ttcaataagg
     5221 caagacgatc aggatcagtg aaaatgtcat ttttggcgta attgacctta atttatgcat
     5281 ggtcttaaca aagacatcat ctttccgttg cagatcttcg ccctttcaaa taacgtactt
     5341 tacaactttc ccgaacaagg agttgtgccc gtgttgtatg ttatttacgc tcaagacaaa
     5401 gctgatagcc tcgaaaaacg cctttccgtt cgtccggcac atttagcacg tttacagtta
     5461 ctgcatgatg aaggtcgttt gttgacagct ggtccaatgc cagcggtaga cagtaacgat
     5521 ccaggggctg cgggatttac tggctcaacc gttattgctg aatttgaatc gctggaagca
     5581 gctcaggcct gggccgatgc tgacccgtac gtagcggcag gagtctatga acacgtttca
     5641 gtgaaaccat ttaagaaagt gttctgaata gagattcaat atttaagggc acggtttttg
     5701 caaccgtgtt cttgtttggc ctttccttat agccggtcag tgcattcact accacaagca
     5761 gataataact ttatctgtat ttctttatat gatgtgagca tattcaggaa aaatgacctt
     5821 tcctgaggta cagttgctcg ctcaaatcct ttgattactg cggataaagt catctactat
     5881 tgtttaacac attcacgcgc tgcatgtatg ttcagaaaat gtgcacgcta agataattaa
     5941 acaaaattgt ggttacagag gtatcagtga gtcactgggc tacattcaaa caaacagcaa
     6001 caaatttatg ggtgaccctt cggcacgaca ttctcgcgct ggccgtcttt ttaaatggat
     6061 tgcttatttt taaaacaatc tatggtatgt cggtcaattt gcttgatatt ttccatatca
     6121 aagcattttc agagttggat ctctccttgc tggcaaacgc cccactattt atgctcggcg
     6181 tctttcttgt cctgaactcc attggcttac tgttccgggc aaagctcgca tgggcaatca
     6241 gtatcatttt gttgttgata gcgctaattt acaccctgca tttttatccc tggctgaaat
     6301 ttagtattgg attttgcatt tttacgctgg tgtttttgct gatactgcgc aaagacttct
     6361 cccacagtag cgccgcagcc gggacaattt ttgcatttat tagtttcacg acattactgt
     6421 tttactcaac ctacggtgcg ctttatttaa gcgaaggttt taatccgcga atagaaagtt
     6481 tgatgacggc gttctatttt tcgatagaaa ccatgtcaac cgtcggctat ggcgatattg
     6541 tccctgtttc tgaatcagca cgattgttca ctatttcggt cattatttcc ggcattaccg
     6601 tttttgccac atctatgacc tcaatttttg gtccgcttat ccgcggggga ttcaacaaac
     6661 ttgtaaaagg aaacaatcat acaatgcatc gtaaagatca ttttattgtt tgcggacatt
     6721 cgattctcgc catcaatacg atcctgcaac tgaatcaacg cggacaaaac gtaacggtta
     6781 tcagcaactt gcctgaagat gatatcaagc aacttgagca acgcttaggc gataacgctg
     6841 atgttatccc cggtgacagt aatgacagtt cagtattaaa gaaagcggga atcgatcgtt
     6901 gccgggccat tctggcgctg agtgataacg atgcagataa cgcgtttgtt gtactctcgg
     6961 caaaagatat gagcagtgat gtcaaaacag ttctcgccgt cagtgatagc aaaaacctga
     7021 ataagattaa gatggtacat ccggatatca ttctctcacc gcaactgttt ggcagcgaaa
     7081 ttctggcgcg agttttaaat ggtgaagaga ttaataatga tatgctcgtt tcaatgttgt
     7141 tgaactcagg tcatggtatt ttcagcgata acgatgaaca agaaacgaaa gctgacagta
     7201 aagaatcggc gcaaaaatag agtttaaaga cggaagagta aacttattgg gcaccgcaat
     7261 ggtgcccagt acatttagat attgtagatc gcaaacaata acgagttacg ctgtttgttt
     7321 agaatgcact tcctgatgct gtggatacgc atatttcggc gcgattgccc ctcaagccaa
     7381 cgcgatttac gtcggctagc ctgacgctgc atgcgccagc gccccacttc cgttctactc
     7441 cgcttcatgt taactactct atgcaataac agaatggtca ttataccccc acccccgacg
     7501 ctgaccagtg cttttcccgc gtttttattt gccagatgaa tccatatgcg taaactcata
     7561 acaatgcgct ttcaaaagga tttctaatct atgacaaccg tttatacgtt ggtgagttgg
     7621 ttggccattc tgggatactg gttgctcatt gcaggcgtaa ctttacgcat tctaatgaaa
     7681 cgacgcgcag ttccctccgc gatggcctgg ctgttgatta tttacattct gccgttagtc
     7741 ggaattattg cctatcttgc cgttggcgag ctccatttag gcaaacgccg cgctgagcgc
     7801 gccagagcga tgtggccttc caccgcaaaa tggcttaacg accttaaagc ctgtaagcat
     7861 atcttcgccg aagaaaatag cagtgtcgct gcgccattat tcaagctttg cgagcgtcgt
     7921 caggggatcg ctggggtcaa agggaatcag ctacaactga tgaccgagtc agatgatgtg
     7981 atgcaggcgt taatccgcga catccagctc gcgcgccata atattgagat ggtgttttat
     8041 atctggcagc ccggcggcat ggcggaccag gtggctgaat cattaatggc ggctgcacgg
     8101 cgtggtattc attgccgatt gatgctcgac tccgccggga gtgtggcttt tttccgcagc
     8161 ccgtggcccg agctaatgcg taatgccggt attgaagtgg tcgaagcctt aaaggtcaat
     8221 ctgatgcgtg tgtttttacg ccgtatggac ctgcgccaac atcgcaagat gatcatgatc
     8281 gataattaca tcgcgtacac cggcagcatg aatatggtcg atcctcgcta cttcaaacaa
     8341 gatgcgggcg tagggcaatg gattgatctg atggcgcgta tggaaggccc catcgccacc
     8401 gcgatgggga ttatttattc ctgcgactgg gagattgaaa ccggaaaacg tattctgccg
     8461 ccaccaccag atgtcaatat tatgccgttt gaacaggcca gcggtcacac cattcacaca
     8521 attgcttctg gccccggctt tccggaagat ctcattcacc aggcattatt gactgcggct
     8581 tattcggcgc gtgaatattt gatcatgacc acgccctact ttgtgccaag cgatgattta
     8641 cttcatgcga tttgcacggc ggcgcagcgc ggggtggatg tcagtattat ccttccgcga
     8701 aaaaatgact cgatgctggt cggctgggcc agtcgcgcat tctttacgga acttcttgct
     8761 gctggggtta aaatttatca gtttgaaggc gggttactgc ataccaagag cgtgctggtc
     8821 gatggcgaac taagtctggt tggcaccgtt aaccttgata tgcgtagtct gtggctgaat
     8881 ttcgagatta ccctggcaat cgacgataaa ggttttggtg cagaccttgc cgccgttcag
     8941 gacgattata tttcgcgttc acgtctgctc gatgcccgtt tatggctaaa acgtccatta
     9001 tggcaacgtg tcgccgagcg actgttttac ttcttcagtc cgttgctgta aaacgtcgcc
     9061 aacagaggtt aaacaggtag tgactatgga tatggatcta aacaatcgcc tgactgaaga
     9121 tgaaacgctt gagcaggcct acgatatttt tctcgaactg gctgccgaca atctcgatcc
     9181 agctgatgtt ctgctgttca atcttcagtt tgaagagcgc ggcggcgcgg agttattcga
     9241 tccggcggaa gactggcagg aacatgttga tttcgatttg aacccagact tttttgccga
     9301 agtggtgatt ggtctggcgg acagtgaaga tggtgaaatc aacgatgttt tcgcccgcat
     9361 tctgttatgc cgcgaaaaag atcacaaact ttgccatatc atctggcggg aataaataat
     9421 cgcttttagc gtgaaaaagg gctgacaact gtcagccctt attgtgttta aagcggatcg
     9481 actttcaggc aagaaacggc gtgtctgaaa ctcccctcca gaacaggacg tgttttggcg
     9541 cactccggac cggcaatcgg gcaacgggta cggaaaacac aaccggaagg cgggttgatc
     9601 ggcgacggta attccccttc cagtaactgg atggttttgt tcttctccag atccggatca
     9661 ggtatgggga ctgccgacat caatgccctg gtgtaaggat gtagtggatt gtggtagacc
     9721 tcatcatagg tccccagttc taccgcatgg ccgagataca tcaccaacac acgatcggaa
     9781 atgtgtttta ccacggccag gtcatgagcg ataaaaatta atgacaatcc catctcacgt
     9841 tgcagctgct ggagcaggtt gaccacctgc gcctgaattg acacgtccag cgccgaaacc
     9901 ggctcatcgc agataatcag cttcggttca agaataagag cacgagcaat cccgatacgc
     9961 tggcactgcc caccggagaa ctcatgcgga tagcggttaa tcaggttagg caataacccg
    10021 actttcagca tcatcgcctt cacgcgctcg cgaacttcct ggcgtgacat tttcggatga
    10081 taagtacgca gtggttcagc gatgatctcg ccgatggtca tacgcgggtt tagcgatgcc
    10141 aacggatcct ggaaaatcat ctgaatatca ctgcgaacgg cacgccattc atcgggcttc
    10201 atgcccagca actctttacc taaccaggca acatgaccgt cggtcgcttt aaccaagcca
    10261 atgatggcgc gagcaaaggt ggacttaccg catcccgatt cccctaccac acctaatgtt
    10321 tccccttcat acaggcgaag agtgacacca tcgacggctt tgagcgtttt cggcggttgc
    10381 cagaaccact gtttgccatc tttgatttca aagtgcactt taagatcggc gatttcaagg
    10441 aggacttttc ttccttcagt tacagcattc ataacagttc ctccaccggt ttaaagcaag
    10501 cacgcagacg gccaggcgta aactcttcca gcggcggtgc gctactacaa atttccatcg
    10561 catgtggaca acgtggctgg aacgggcaac ctttcggtaa tcgcagcaag tttggcggat
    10621 tacccgggat ggtcaacatt gtttcacctt ccgcatcaag acgcggcacc gcgttgagca
    10681 aaccgataga ataaggatga acgggttgat aaaagacatc gcgcgcattg ccatattcca
    10741 tcgtgcgccc ggcgtacatt accagcactt tgtcgcagat ccccgccacc acgccaagat
    10801 cgtgggtgat cataatgatg gcggtattaa attcccgctt cagttcattt aatagcgtca
    10861 tgatctgcgc ctgtacggtg acgtccagcg cagtagtggg ttcatccgca atcagtagct
    10921 taggtcgaca tagcaatgcc atcgcaatca tgactcgctg acgcatgccg ccagaaaatt
    10981 cgtgcgggta cattttcatg cgtttacgcg cttccggcat ttttaccgca tcgagcatcc
    11041 gcaccgactc ttcaaacgcc tcagctttgc tcatgttctt atgcagcatc agcacttcca
    11101 ttaactgctc accgacgcgc atatagggat tcaacgaagt cattgggtcc tgaaaaatca
    11161 ttgagatttg ttcagcgcgc agtttattga gttcatgctc tggcaaattg aggatttcac
    11221 gcccattgaa ggttgccgat ccgccaatac gtccgttggc agccaacagg cccatcaacg
    11281 caaatgcagt ttgcgattta cccgaaccag actcacctac aatacccagc gtttctccgg
    11341 cacgtaggga aaaattcaaa tcattgaccg ccgtgacgtc gccgtccggg gtactaaagg
    11401 tgacacgcaa atctttcacg ttcagcagtg cgtcagcctg ttgttgtgcg agcggcacag
    11461 ttgcagtttc aattacgctc atggctgcac tccttaacga tctttcgggt cgagggcatc
    11521 acgcaagcca tcgccgataa agttgaaaca aaacagcgtc accacgagga atcccgctgg
    11581 gaacaacaat aaccatggag agacttccat cgagttcgcg ccatcactca gcaatgcacc
    11641 ccagctgctt aacggctctt gcgtacccaa ccccaggaag ctaaggaaag attcaaagag
    11701 gatcatgctg ggcaccagaa gtgatgcgta gaccaccacc acaccgagta cattcggcac
    11761 aatgtggcga ataacaatgc ccgatgtcga tacaccgcca acttgtgccg cctcaataaa
    11821 ctctttgcgc ttcagactca gggtttgccc acgcacaata cgagccatat ccagccagga
    11881 aaccatgcca atcgccacga aaatcagcag gatgttttga ccgaaaaagg tcacaagcaa
    11941 aatgacgaag aacatgaatg ggaaggagtt gaggatttcc agcagacgca tcattaccga
    12001 atccacttta ccgcccagat aaccggaaag cgaaccataa agtgtcccga cgaccacagc
    12061 caccagtgcc gcagcaacac cgaccatgag tgagatacgc ccgccaatcg caacgcgcac
    12121 aagcaggtcg cgaccggatg agtcagtacc aaagtagtga ccggactcca tatccggggc
    12181 gctggacatc atcgcccagt cagtatcgtc ataggcaaac tgggaaagca tcggtgccag
    12241 gattacaaat aacgcgatca gcaccagcac tatcagactg gcaaccgccg cacggttatg
    12301 cataaaacgt cgacgtgcgt cctgccacaa gctgcgccct tcgacctcca gcttttcact
    12361 gaaattttcc agcgtctcgc tgtttttctt acttaacatc atcgcgagct ccagtatcag
    12421 taacgaattt tcgggtcgat aaccgcatat agcacatcga caatggcatt aaacaaaatg
    12481 gttaaagcac caaccaggat ggtcaggctt aacactaagg aatagtcacg gttcaatgcg
    12541 ccattaacga acagttgtcc aatccccggc aaaccataaa tggtttcgat aaccatagaa
    12601 ccggtaataa tgccgacaaa tgcggggccc atataagaga gcacgggtaa cagagcaggt
    12661 tttaatgcgt gacgtaaaat gatccgccgc ataggtaacc ctttcgcccg ggcagtacga
    12721 ataaaattgg agtgtaatac ttcaatcata gagccacggg taatacgcgc aatactggcg
    12781 atataagcga gtgacaacgc caccatcggc aatatcatga atttaagcgc cccaccattc
    12841 cagccaccgc ccggcagcca atgcaaaatg atcgcaaata tcatgactaa taatggtgca
    12901 accacaaaac tggggataac aaccccggtc attgccagcc ccatcacggt atagtcccat
    12961 ttggtgtttt gttttaatgc ggcaataacg ccagcactaa cacccagtat taccgcaagg
    13021 aaaaatgctg cggctcccag tttggcagaa acgggaaaac tggatgcaac caagtcattg
    13081 accgaataat ctttatattt aaacgatgga ccgaaatcac cgtgcgccag ttgtttcagg
    13141 tagctgaaat actgtgtcat gatcggatca ttaagatgat atttcgcttc gatattggcc
    13201 atcacttctg gcggtaaagt acgttcgccg gtaaaagggc ttcccggcgc gaggcgcatc
    13261 ataaagaacg aaatagttat aagaataaat agcgtcggaa tcgcttccag acagcgacgt
    13321 agaataaatt ttaacattgc cctaccttct ggcctgtgcc ttctgtaatg cgataaaaat
    13381 cagacaccgt ggagcaggac actcctgccc cacgtattgc cattagtgct tcacaatgta
    13441 catattccgg gtataggtat tatccagcgg atctttgccg gtatagccac caacccacgg
    13501 tttcaccaga cgcgcattca cgtagtaata aacaggaaca atggccgaat ccttatccag
    13561 ctgttgttct gctttagtgt acagagctgt gcgccgcgcc tcgtcagtca ctttcagcgt
    13621 ttccgccata atgctgtcaa aggccgggct cttataatgc gcggtattca tcgagctgtt
    13681 cgaaagcatg gtgttcagga aggaagttgg ttcgttgtag tcagcacacc agcctgcacg
    13741 ggccacatca aaagtaccct ggtgacgggt gtcgaggaac gttttccact cctggttaac
    13801 cagtttgacg tttacaccaa tgtttttctt ccacaatgaa gaggcagcaa tcgccagctt
    13861 tttatgcaga tcggaggtgt tatacaacag gttgatggtc aacggtttgt ctgcggtata
    13921 acccgcttca gccagcagtt ttttcgcttc ttcgttacgt ttttcctggc tccagccaaa
    13981 ccattccggc tgagtcaatt ttgcgccatc agtatacggt ggagtgtaac cataggcggg
    14041 catgtcgccc tgcgctttca ctttattaac aatgatatcg cggtccatac ctagtttcag
    14101 tgcggtacgc acacgcacat cgttgaatgg cggtttctgg ttgttaattt cgtaatagta
    14161 agtgcacagg tatggatcaa cgtgaacttc gtccgggatc tcttttttca gcttctggaa
    14221 caattcgatc ggcatgttgt tataagtcat gtcgatttca ccactacggt agcggttgac
    14281 atcggtaact tcagaagcaa taggcaaata ggttacctgg ttaataacgg ttttcgcgtt
    14341 gttccagtag gtcgggctgc gttcaagaac gattcgttcg tttacgaccc aatcttttaa
    14401 ggtataggca ccgttggtga cgatattacc aggctgggtc catttttcgc cgaatttctc
    14461 gatagcggct tttggcaccg gtgaagttga tgggtgaaca agtaatttat agaagtacgg
    14521 aacgggttca cttaaggtga cttctaatgt gtgatcatca atagctttca cgccgagatc
    14581 ggtaatcggt tttttccctt caagaatttc atcaataccg gcgatatgcc catattgcag
    14641 ataactggca tacggagaag cagtgttcgg atcaacagaa cgttgccagc tatacacaaa
    14701 gtcttgtgct gtgactggcg tgccatcaga ccatttcgca tctttacgca aatggaaggt
    14761 ccagactttc gcgtctttat tatcccagga ttcagcgacg ccaggtgctg gatgaccgtc
    14821 aagatcgctg accagtaagc cttcaaacag gtctcggctg atattagact ccggaacacc
    14881 ttcaattttg tgcggatcta atgactgaac ttctgaacca ttgttacgta ccagtgtttg
    14941 tttttccgcc agtgtgacgc ctgcgggtac atcagctgcc agcgcgacat tccctgccat
    15001 tagcgcagcc agaacgccag ctgctactaa acttctcttg gtgatgttgg tcattgtttt
    15061 ttggactccc tcattataat tactggtatt aaccagcatg tgtaatcccg gattgggacc
    15121 ctgcacaggc tcggagactt tctgctgtca ggttacttta tcgttacgta ttactatcac
    15181 cgactttatt tattctggtg attcagacga tcaccttatg tcgattcgta ctgtgtctcc
    15241 cctgtcgaga cacattattc tttcgcgacg ctctttaatt gttaattatt ctcattacca
    15301 ataaagaatc gtctggcggt tgcccggaaa gtaccaaatg cggttcttac tcgccaatac
    15361 attttgcaaa attgttaagc aattctcttt tattgaccgt atcagaatct atagcttgca
    15421 tccatgcctt aacaaacatc tttcaattca atcaaataga gattggctaa gcatgtctat
    15481 cttttctgat tacagttcca gtagtgaaat gcacaataat ttaacaattg attattattt
    15541 agcactttcc tctaccaaag gcagtggaat tactaatatc atttcaatta ttcttcagca
    15601 agcccaggat tatgatgtgg cgaaaataac atgatggtga ggagaagcag ttaaggaaat
    15661 ccttataact aactgtaatt tgagaagaat atttttacct attccttttg cttttgataa
    15721 ctgcgcggga acaaagtaaa aataatgacg aagtaaacgg agcatggcag ctccgtttca
    15781 tttgaaagga attaattaag caggccgggg aaaatcccct taataccagt aacgataaat
    15841 tcaatcccca atgccatcag caatagcccc ataatacgcg taatcacgtt gatgccggtc
    15901 tggcgtaaaa cccgtaccag ccacggtgcc atgcggaaca atccccagca acataaagcg
    15961 aacaatgcaa tagccacaaa gaaaccaaac agatagctaa tgctgtgata acgcgtaccc
    16021 cagacgatgg tagaactgat cgcccctggc cccgccatca acggcaacgc caatggcacc
    16081 acaccaatgc tttcacgtac cgcggtttct gatttttctt gcttgttctg tttatcctcg
    16141 ccaagcttgc cgctgatcat cgacatcgct atcgtcacca ccaggatgcc cccggcgata
    16201 cggaacgaat caattgatat accaaaaagt tgcagaatcg tgtcgccgag aaaaagcgag
    16261 atccacaaga taatggccac agacaggttg gctgtaaggt tagttttgtt tcgcgctgcc
    16321 gctgtctgat aactggtcat gctgataaag acgggaataa tccctaccgg gttgaccagc
    16381 gcaaataacc cgatgaaaaa tttgaagtaa acgggaaaat caaaaaaggt ctgaatcacg
    16441 gttagctccg aagcaaaagc cggataatgt tagccataaa taaggttgaa aagacgcgct
    16501 gacaatacgc cttttgacag catttttcac ctcctaacta cttaaaattg ctatcattcg
    16561 ttattgttat ctagttgtgc aaaacatgct aatgtagcca ccaaatcata ctacaattta
    16621 ttaactgtta gctataatgg cgaaaagcga tgctgaaagg tgtcagcttt gcaaaaattt
    16681 gatttggatc acgtaatcag tacccagaag tgagtaatct tgcttacgcc acctggaagt
    16741 gacgcattag agataataac tctaatgttt aaactctttt agtaaatcac agtgagtgtg
    16801 agcgcgagta agcttttgat tttcataggt taagcaaatc atcaccgcac tgactatact
    16861 ctcgtattcg agcagatgat ttactaaaaa agtttaacat tatcaggaga gcattatggc
    16921 tgttactaat gtcgctgaac ttaacgcact cgtagagcgt gtaaaaaaag cccagcgtga
    16981 atatgccagt ttcactcaag agcaagtaga caaaatcttc cgcgccgccg ctctggctgc
    17041 tgcagatgct cgaatcccac tcgcgaaaat ggccgttgcc gaatccggca tgggtatcgt
    17101 cgaagataaa gtgatcaaaa accactttgc ttctgaatat atctacaacg cctataaaga
    17161 tgaaaaaacc tgtggtgttc tgtctgaaga cgacactttt ggtaccatca ctatcgctga
    17221 accaatcggt attatttgcg gtatcgttcc gaccactaac ccgacttcaa ctgctatctt
    17281 caaatcgctg atcagtctga agacccgtaa cgccattatc ttctccccgc acccgcgtgc
    17341 aaaagatgcc accaacaaag cggctgatat cgttctgcag gctgctatcg ctgccggtgc
    17401 tccgaaagat ctgatcggct ggatcgatca accttctgtt gaactgtcta acgcactgat
    17461 gcaccaccca gacatcaacc tgatcctcgc aactggtggt ccgggcatgg ttaaagccgc
    17521 atacagctcc ggtaaaccag ctatcggtgt aggcgcgggc aacactccag ttgttatcga
    17581 tgaaactgct gatatcaaac gtgcagttgc atctgtactg atgtccaaaa ccttcgacaa
    17641 cggcgtaatc tgtgcttctg aacagtctgt tgttgttgtt gactctgttt atgacgctgt
    17701 acgtgaacgt tttgcaaccc acggcggcta tctgttgcag ggtaaagagc tgaaagctgt
    17761 tcaggatgtt atcctgaaaa acggtgcgct gaacgcggct atcgttggtc agccagccta
    17821 taaaatagct gaactggcag gcttctctgt accagaaaac accaagattc tgatcggtga
    17881 agtgaccgtt gttgatgaaa gcgaaccgtt cgcacatgaa aaactgtccc cgactctggc
    17941 aatgtaccgc gctaaagatt tcgaagacgc ggtagaaaaa gcagagaaac tggttgctat
    18001 gggcggtatc ggtcatacct cttgcctgta cactgaccag gataaccaac cggctcgcgt
    18061 ttcttacttc ggtcaaaaaa tgaaaacggc tcgtatcctg attaacaccc cagcgtctca
    18121 gggtggtatc ggtgacctgt ataacttcaa actcgcacct tccctgactc tgggttgtgg
    18181 ttcttggggt ggtaactcca tctctgaaaa cgttggtccg aaacacctga tcaacaagaa
    18241 aaccgttgct aagcgagctg aaaacatgtt gtggcacaaa cttccgaaat ctatctactt
    18301 ccgccgtggc tccctgccaa tcgcgctgga tgaagtgatt actgatggcc acaaacgtgc
    18361 gctcatcgtg actgaccgct tcctgttcaa caatggttat gctgatcaga tcacttccgt
    18421 actgaaagca gcaggcgttg aaactgaagt cttcttcgaa gtagaagcgg acccgaccct
    18481 gagcatcgtt cgtaaaggtg cagaactggc aaactccttc aaaccagacg tgattatcgc
    18541 gctgggtggt ggttccccga tggacgccgc gaagatcatg tgggttatgt acgaacatcc
    18601 ggaaactcac ttcgaagagc tggcgctgcg ctttatggat atccgtaaac gtatctacaa
    18661 gttcccgaaa atgggcgtga aagcgaaaat gatcgctgtc accaccactt ctggtacagg
    18721 ttctgaagtc actccgtttg cggttgtaac tgacgacgct actggtcaga aatatccgct
    18781 ggcagactat gcgctgactc cggatatggc gattgtcgac gccaacctgg ttatggacat
    18841 gccgaagtcc ctgtgtgctt tcggtggtct ggacgcagta actcacgcca tggaagctta
    18901 tgtttctgta ctggcatctg agttctctga tggtcaggct ctgcaggcac tgaaactgct
    18961 gaaagaatat ctgccagcgt cctaccacga agggtctaaa aatccggtag cgcgtgaacg
    19021 tgttcacagt gcagcgacta tcgcgggtat cgcgtttgcg aacgccttcc tgggtgtatg
    19081 tcactcaatg gcgcacaaac tgggttccca gttccatatt ccgcacggtc tggcaaacgc
    19141 cctgctgatt tgtaacgtta ttcgctacaa tgcgaacgac aacccgacca agcagactgc
    19201 attcagccag tatgaccgtc cgcaggctcg ccgtcgttat gctgaaattg ccgaccactt
    19261 gggtctgagc gcaccgggcg accgtactgc tgctaagatc gagaaactgc tggcatggct
    19321 ggaaacgctg aaagctgaac tgggtattcc gaaatctatc cgtgaagctg gcgttcagga
    19381 agcagacttc ctggcgaacg tggataaact gtctgaagat gcgttcgatg accagtgcac
    19441 cggcgctaac ccgcgttacc cgctgatctc cgagctgaaa cagatcctgc tggataccta
    19501 ctacggtcgt gattatgtag aaggtgaaac tgcagcgaaa aaagaagccg ctccggctaa
    19561 agctgagaaa aaagcgaaaa aatccgctta atcagtagcg ctgtctggca atataaacgg
    19621 ccccttctgg ggccgttttt ttgtttaccc aaagcaactt ttccataaac cgacagcatt
    19681 agccttcatc atatttgcga cgatgtataa cgcctaaaca cagggatatt gtactttata
    19741 ggtcacaagt caacgtcggt gcttaagagc cctgtgaggc gtatagcggc gttaaaaaac
    19801 tgccgagaag ggtatatagc ccggaagaag tgcgtaaaac gaactgacag gataaaagtg
    19861 ccctgctcac cctgtcagta aagaaattct tattaatcgt ggcgatgcct ttcctgaata
    19921 gccgttaatg agccgacttg taacgcctct ttatagtgtt tacggcatac agaaacgtat
    19981 cgttcattac caccaattac cacctgctca ccttcgttat aaggtctgcc tgcttgatca
    20041 agacgcagca ccatgcttgc tttacggcca caaaaacaga tggtttttaa ttcaaccagt
    20101 ttgtcggacc atgccagtaa gtattggctg ccaataaata attcacctcg aaaatcggta
    20161 cgtaaaccat aacaaagtac gggtatatcg agttgatcga caacctccga taattcatat
    20221 acttgttgtc tggttaaaaa ctggcattca tcaaccagta cgcaatgaat tgcctgctgt
    20281 tcatgttccg cacgaatctc atcaaataat gatgaatttt ggttaaataa ttttgcaggc
    20341 gatgacaaac ctatacgcga actgactttc ccggcaccaa agcgatcatc aatttctgcc
    20401 gtatatacga cagtgcgcat gccgcgttcc tggtaattgt atgaagattg caacaatgct
    20461 gtagacttac ccgcattcat tgcggaatag tagaaatata gctgtgccat cagccacagg
    20521 ccctcaatga tatgaaataa agttggctgg agtttatcat aattcgtcag tttttcagct
    20581 caacagtatg cacagaagtg ggtatatgcg ttctccctta cgaagccttg cataatcctt
    20641 ctgagctatc attacaactg cctcgcttgt tataagcggg taaatgactg ctggtaacta
    20701 ttcacaatct ttaacctgtt gcgcaagtaa tagccctctg ttgacctcca ggagatagtg
    20761 caatactaag tccatgctct tattgcgact gttctacttt tcatcattcg cttaataggg
    20821 aattctcgta aacacaacta atacagaaga ctgaaaggtc gtcagcctac gataatctcc
    20881 ccataaaatg tgacatgaat caggaagttt taacctcacg tgctgcgaaa tcatcggtgt
    20941 aaatagggct atatgccgcg tcttttctgg ctaattttat gaaaagatat ttattggcgg
    21001 cacaaaataa agaacaattt tgaattcctt acattcctgg ctattgcaca actgaattta
    21061 aggctctatt attacctcaa caaaccaccc caatataagt ttgagattac tacaatgagc
    21121 gaagcactta aaattctgaa caacatccgt actcttcgtg cgcaggcaag agaatgtaca
    21181 cttgaaacgc tggaagaaat gctggaaaaa ttagaagttg ttgttaacga acgtcgcgaa
    21241 gaagaaagcg cggctgctgc tgaagttgaa gagcgcactc gtaaactgca gcaatatcgc
    21301 gaaatgctga tcgctgacgg tattgacccg aacgaactgc tgaatagcct tgctgccgtt
    21361 aaatctggca ccaaagctaa acgtgctcag cgtccggcaa aatatagcta cgttgacgaa
    21421 aacggcgaaa ctaaaacctg gactggccaa ggccgtactc cagctgtaat caaaaaagca
    21481 atggatgagc aaggtaaatc cctcgacgat ttcctgatca agcaataatc ttttgtagat
    21541 tgcacttgct taaaatcccg ccagcggcgg gattttttat tgtccggttt aagacaattt
    21601 aataagaata agctataaaa aaacggcgtc gattgctcaa cgccgtttcg tggataacac
    21661 cgatacggat gttacttctt aatgcccatc tcttcttcaa gccaggcttt aaattccgtg
    21721 ccaagggtgt tatgacgaat accgtattca acgaaggcct gcatgtaacc taatttatta
    21781 ccgcagtcat ggctcttccc tttcatatga taggcttcca ccgtttcttt ttcgatcagc
    21841 atatcaattg cgtcggtgag ctgaatttca tcaccagctc ccggaggggt ttttgccagc
    21901 aacggccaaa tatccgcgct aagtacgtaa cgacccacaa tagcgagatt agacggcgca
    21961 acatccgctt tcggtttttc taccacacca accatcggta cgctttcacc cggcgctaat
    22021 tcaacgcctt tgcaatccac aacgccatat gcggtcacat cagcaaccgg ttcaaccatg
    22081 atctggctat gacccgtttc atcaaagcgg cggatcatct ctgccaggtt atcctgtgac
    22141 aaatcggatt catattcatc cagaataaca tcaggcaaaa taacagctac cggttcatca
    22201 cccactaccg ggtgagcaca caataccgcg tgtcccaggc ctttcgccag cccctgacga
    22261 acttgcataa tagttacgtg cggcggacaa atagactgca cttcatcaag cagttgacgt
    22321 tttacacgtt tttccagcat tgcttccagt tcaaaactgg tatcaaagtg gttttcaata
    22381 gagtttttag atgagtgagt aaccagcaca atttcagtaa tgccagccgc aatacattca
    22441 ttcacgacgt attgaattaa tggcttatcg acaagtggca gcatctcttt cgggatggct
    22501 ttcgtcgccg gcaacatcct ggttcctaat cccgcaacgg ggataacggc ttttttgact
    22561 ttcgtattaa tggcagccat ttaaattctc ctggactgtt cgtgttttga acgtgttcat
    22621 atttctgtat cgcatcccag tatatcagca ccccgaaggg gctaagttct gaaaaggagg
    22681 cgagttagca taattaagac aattacttat acgaatggcg acgatagtac caccagccga
    22741 cattagcagg taatgcaaat ttagcccgcg ttatcgtttg ctcattctgc agacaacatc
    22801 aagcgcagtc gaccaccggt tccccatatt tggcattgcc aggcatcgca tcgctggctc
    22861 aattgattca aataagcgtt acctaaagtg cctaacggaa caccattact gatttgcacc
    22921 tggtgttcgc cggtatttaa cgtcgcattc agacccgcag aaaccagaat gagatttttc
    22981 agttcgcgat gataatagcc aactaatagc ggaaactgcc ccggcagatt ggcctgacgg
    23041 agtaaatggt ttacctgctt caataacgcg cccaactctg gcaaccgttg attttggtgt
    23101 gcaagctgtt cctgtaataa tccgttaaac aatgcgcgta ataacaaggc agcaagtacg
    23161 ccattatgtc cagctcgagt gacatcaagg caataaaatg ccagatcgtt ttccgaaagt
    23221 gcggcaatat caagcaccag gccgggtttg tccgcggcaa ccaattgacg ataattaacc
    23281 cggcaatggg aaatcacctg ctgaaccggc ggttgtagtt cctgtaataa tttcgccgct
    23341 gcggcagggt tatcaaccat tgcatcccag tcgcgaaaaa gcctttcctc ttcctcaacg
    23401 cgcgaattaa acatgctggg atagagacag gcaaaaacca tctcgcgcaa gcgattcaga
    23461 tctttaactg gtttcagcaa aacatcttca acgcccagac gtaacgcttt ggcaatatct
    23521 gccatatttt cagtggcaga tatcaccaga actggggtct ggtcgcctct gttacgtata
    23581 tgctccagca gtttaagccc gttcattcgt ggcatcgcga tatcacatat catcaggtct
    23641 ggagtgaaac ctcccagcaa ctcaagggca tccaccccat cagccgccag taccgttgtc
    23701 gctcccaatg aggaaaacca tgaatccaga agcgagcgaa atacctgctc atcttcaaca
    23761 atgagaatct gttttccgac caatggctgc gtcatgttct ctcccctgac tggctttact
    23821 caatagtggc atgctattgc cacctgcgcc tgtcagaatt aacttaagtg tagataaaaa
    23881 ttctggtcaa atgttggtgc gtaccaacgg caaaagttcg tccattttcc tttccactgc
    23941 cagctgtccg gccgcaatgg cagcgtgcgc gcgatggaaa tcaagcgtag atatttgcgg
    24001 gcaaacaggt tgaatcagaa tatcgggcgg atcacctgcc atgcggttcc ttttaaggcg
    24061 gttctccagc acctggatag aagtggtcat aatctctgtt gccgttggcg ctgtcaccgc
    24121 ccgacgtgtc gttatgctgc ctaacctttc tttcagacgc gcatgccacg gcagagaatc
    24181 accattctcg ctattttctt cactgacatt aaaggagagc aaatcttgtt gcatcaaatg
    24241 agcatcgtgc tgcaggtcaa ccgctatcac aatatcagcc cccaatgcac gcgtgaggga
    24301 aataggaatt gggttaacga ctgctccatc aaccagccag tagccgttat gtgcaacagg
    24361 tgccatgagt cctggaatac tgcatgatgc gcgaatagca agatggagat cgccttcagt
    24421 aaaccataat tcacgtcccg tacttaaatt ggtggcaaca gccgcaaagc gacgggaaca
    24481 attttcgatc tctgtttccg gcattatttc gcggtattga ttgaagacac gctcgccgcg
    24541 cagtaaccca ccgcgctgcc aggagagatc catcaggcgt aaaacatccc aataactgaa
    24601 agaggtcacc caatcttcca gcgcagataa tcgatcgcat gcataggcag cgcccaccag
    24661 cgaaccaatt gaacatcctg caacgatatc aatttcaata cccacttttt ttagcgcatt
    24721 aataacgcca atatgcgacc aacctctcgc cgcgccagat cccagcgcca gccctatttt
    24781 tatctttctc attattcccg caagattccc ctggaatgca atcgtagcca catcgctact
    24841 gctcggttaa catagtgcta ctcaggcggt taatacgccg ttattgtttc cagggagatt
    24901 atttgtgtct cagctttgtc cctgtggtag tgctgtcgag tatagcctat gttgccaccc
    24961 ttatgtgtct ggtgaaaagg ttgcacctga tccagaacat ctcatgcgtt cgcgttactg
    25021 cgcttttgtg atgcaagacg cagattattt aataaagacc tggcatccct cttgtggagc
    25081 agcggcgtta cgtgccgaat tgatggccgg atttgcgcat actgaatggt tgggattgac
    25141 cgtatttgaa cattgttggc aggacgccga caatatcgga tttgtcagtt ttgttgctcg
    25201 atttaccgaa ggtggtaaaa caggtgcaat aattgaacgt tcgcgatttt tgaaagaaaa
    25261 cggtcaatgg tactatattg acggtacacg tccgcagttt ggtcgcaacg atccctgccc
    25321 ttgtggttca ggtaaaaaat ttaaaaagtg ctgcggccaa taatggttga cggtacggtt
    25381 tagcaaacac tctcaacaag gtttttccag caatgcattc actccaacgt aaagttctgc
    25441 gtactatttg tccggaccaa aaaggtctga tcgcacgtat taccaatatt tgctacaagc
    25501 acgagttaaa tatcgtacag aacaatgaat ttgttgatca ccgtaccggg cgctttttta
    25561 tgcgcacgga actggaaggg atttttaatg attccaccct gctggcggat ctcgatagcg
    25621 cattgccaga aggctccgtg cgtgagctga atcctgccgg tcgtcgccgg atagtgattc
    25681 tggtcactaa agaagcgcat tgccttggcg atttgttgat gaaagccaat tacggcggcc
    25741 tggatgtcga aatcgcggca gttattggta accacgatac tttacgttct ctggttgagc
    25801 gttttgatat tccgtttgag ctggtaagcc atgaagggtt aagccgcaac gagcacgatc
    25861 aaaagatggc ggacgccatt gatgcttatc aacctgacta tgtggtgctg gcgaagtata
    25921 tgcgggtatt aacgccggaa tttgtggcac gcttcccgaa taaaatcatc aatattcacc
    25981 attcattcct gcctgcgttt atcggcgcac gtccttatca ccaggcctat gaacgtggtg
    26041 tgaagattat tggcgcaacc gctcactatg tgaatgacaa tctggacgaa ggcccaatca
    26101 tcatgcagga cgttattcat gtcgatcata cctacacagc tgaagatatg atgcgcgcag
    26161 gtcgtgacgt cgagaaaaac gtcttaagtc gtgcgctata caaagtactg gcacagcgcg
    26221 tctttgttta cggtaatcgg acgattattc tttaatcgcc agcaaaaata actggttacc
    26281 tttaatccgt tacggatgaa aattacgcaa ccagttcatt tttctcaacg taacacttta
    26341 cagcggcgcg tcatttgata tgatgcgccc cgcttcccga taagggagca ggccagtaaa
    26401 aagcattacc ccgtggtggg gttcccgagc ggccaaaggg agcagactgt aaatctgccg
    26461 tcatcgactt cgaaggttcg aatccttccc ccaccaccat cacttttcaa aagtccgtaa
    26521 actctcaaac gaatccgcaa ttaaatattc tgcccaagcg gggaaggatg agaagcttcg
    26581 accaaggttc gacttgagc
//
LOCUS       DAZFXT010000060        26496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.060, whole genome shotgun sequence.
ACCESSION   DAZFXT010000060 DAZFXT010000000
VERSION     DAZFXT010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.060"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(26..1006)
                     /gene="ygfZ"
                     /locus_tag="ACHKW0_002705"
     CDS             complement(26..1006)
                     /gene="ygfZ"
                     /locus_tag="ACHKW0_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HFO7475511.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            1249..1515
                     /gene="sdhE"
                     /locus_tag="ACHKW0_002706"
     CDS             1249..1515
                     /gene="sdhE"
                     /locus_tag="ACHKW0_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HFO7475512.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            1496..1903
                     /gene="ygfX"
                     /locus_tag="ACHKW0_002707"
     CDS             1496..1903
                     /gene="ygfX"
                     /locus_tag="ACHKW0_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HFO7475513.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(1943..2464)
                     /gene="fldB"
                     /locus_tag="ACHKW0_002708"
     CDS             complement(1943..2464)
                     /gene="fldB"
                     /locus_tag="ACHKW0_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HFO7475514.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            2576..3472
                     /gene="xerD"
                     /locus_tag="ACHKW0_002709"
     CDS             2576..3472
                     /gene="xerD"
                     /locus_tag="ACHKW0_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HFO7475515.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            3497..4207
                     /gene="dsbC"
                     /locus_tag="ACHKW0_002710"
     CDS             3497..4207
                     /gene="dsbC"
                     /locus_tag="ACHKW0_002710"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HFO7475516.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSAAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            4213..5946
                     /gene="recJ"
                     /locus_tag="ACHKW0_002711"
     CDS             4213..5946
                     /gene="recJ"
                     /locus_tag="ACHKW0_002711"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HFO7475517.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVVHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            6037..7135
                     /gene="prfB"
                     /locus_tag="ACHKW0_002712"
     CDS             join(6037..6111,6113..7135)
                     /gene="prfB"
                     /locus_tag="ACHKW0_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HFO7475518.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            7145..8662
                     /gene="lysS"
                     /locus_tag="ACHKW0_002713"
     CDS             7145..8662
                     /gene="lysS"
                     /locus_tag="ACHKW0_002713"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7475519.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(8705..9253)
                     /gene="idi"
                     /locus_tag="ACHKW0_002714"
     CDS             complement(8705..9253)
                     /gene="idi"
                     /locus_tag="ACHKW0_002714"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HFO7475520.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            9308..9379
                     /locus_tag="ACHKW0_002715"
     CDS             9308..9379
                     /locus_tag="ACHKW0_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475521.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(9376..9501)
                     /gene="yqfG"
                     /locus_tag="ACHKW0_002716"
     CDS             complement(9376..9501)
                     /gene="yqfG"
                     /locus_tag="ACHKW0_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqfG"
                     /protein_id="HFO7475522.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(9503..10951)
                     /gene="uacT"
                     /locus_tag="ACHKW0_002717"
     CDS             complement(9503..10951)
                     /gene="uacT"
                     /locus_tag="ACHKW0_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HFO7475523.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            11387..13306
                     /gene="ygfT"
                     /locus_tag="ACHKW0_002718"
     CDS             11387..13306
                     /gene="ygfT"
                     /locus_tag="ACHKW0_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HFO7475524.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            13306..13794
                     /gene="ygfS"
                     /locus_tag="ACHKW0_002719"
     CDS             13306..13794
                     /gene="ygfS"
                     /locus_tag="ACHKW0_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HFO7475525.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(13830..15197)
                     /gene="ghxQ"
                     /locus_tag="ACHKW0_002720"
                     /gene_synonym="ygfQ"
     CDS             complement(13830..15197)
                     /gene="ghxQ"
                     /locus_tag="ACHKW0_002720"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HFO7475526.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(15233..16549)
                     /gene="guaD"
                     /locus_tag="ACHKW0_002721"
     CDS             complement(15233..16549)
                     /gene="guaD"
                     /locus_tag="ACHKW0_002721"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HFO7475527.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(16567..17967)
                     /gene="xanQ"
                     /locus_tag="ACHKW0_002722"
     CDS             complement(16567..17967)
                     /gene="xanQ"
                     /locus_tag="ACHKW0_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HFO7475528.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(18132..21002)
                     /gene="xdhD"
                     /locus_tag="ACHKW0_002723"
     CDS             complement(18132..21002)
                     /gene="xdhD"
                     /locus_tag="ACHKW0_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HFO7475529.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNSDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(20999..21778)
                     /gene="ygfM"
                     /locus_tag="ACHKW0_002724"
     CDS             complement(20999..21778)
                     /gene="ygfM"
                     /locus_tag="ACHKW0_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HFO7475530.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            complement(21829..23157)
                     /gene="ssnA"
                     /locus_tag="ACHKW0_002725"
     CDS             complement(21829..23157)
                     /gene="ssnA"
                     /locus_tag="ACHKW0_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HFO7475531.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLHEAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(23160..26258)
                     /gene="ygfK"
                     /locus_tag="ACHKW0_002726"
     CDS             complement(23160..26258)
                     /gene="ygfK"
                     /locus_tag="ACHKW0_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HFO7475532.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVQGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVADHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ERFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNDKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLAQDFDNSS
                     NPGFLVEDCRVRVRLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
BASE COUNT         6577 a         6704 c         6954 g         6261 t
ORIGIN      
        1 taaatgcctg atgcgataaa gagatttact cttcgagcga atacggcagc ggctcgatat
       61 gcagtgtatt cgcatcgtcg cgtacgcgga agatgctatc cggttccata tcgttattca
      121 tgacgacctg taccacgacc tgaccatctt ccagttttac cgcagccagc accgtaccgg
      181 tacgacgcca gttctcgccc attttcagct ctaagtcttc accagcttcc ggcagtcggc
      241 tggcgctacc tgccagcaac cagagcgcac gtttattggc accacggaat tttgctcgcg
      301 ccaccatctc ttgtccggta tagcagcctt ttttaaagct gatgccgccc agcgcctgga
      361 ggttggtcgc ctgtgggata aactgcccgc tgttggcggc atcaatcacc gggaaacccg
      421 cttcaatgtt taatgccagc cactgttggc tattgttcag ttccgcttca ccgcgcagtt
      481 tatcggtcag catattagca gtagcttcat cggttacgat caggaaacgt tctgccgggt
      541 gttcaaacca tagcaaagtg gtcgcgcctt ctttaactac ctgtttttct ttcgaaggca
      601 gttcactaaa gagatttgcc agcgcggcgc gcgcctgaaa accggcaaca ccaagcagca
      661 cacgctcgtc gtctggcgcg atggtcactt tagagaatac cgcatatttt ttcagttcag
      721 tcagctgcgg ttcacgcacg ctgcgccgtt caatccatgc aaagccatcg ccgtcgcgga
      781 acagacgtaa attgctccac attttacctt tggcgtcgca atgggcggcg agcaggtgct
      841 gatcttctgc catctggctg acatctgctg tcacctgacc ctgcatatat ttttcgctgt
      901 ccgcgccagt aatggtggca agcgcccagt catcaagcgt catcagcgtc agcggtaaac
      961 gggcagaagc cgtaggctga cggggaggaa aaggtgtaaa agccataata gagtcctgag
     1021 ttgcttaacg gcgtattaat gactaatggt aaaagagcta tgaggcaatg caagtgcttt
     1081 cactacgcga tctgtgcctt ctgcgaggcg acttccaaaa aaatgaagcc attgaattgt
     1141 tgtgaattaa tttcgcgatc gcgatggtgt ttactgatat tcccgtaaaa gaaggcgaaa
     1201 aacacgttac aatagccggg tactggattt tcgtgagaca ggaagaagat ggacattaac
     1261 aacaaagccc gcattcattg ggcatgccgc cgtggtatgc gcgaactcga tatttcaatc
     1321 atgccgtttt tcgaacatga gtacgacagc ttaagcgatg acgaaaaacg catctttatt
     1381 cgtctgctgg aatgtgacga tccggacctg tttaactggc tgatgaatca cggtaaaccg
     1441 gccgatgcag aactggaaat gatggtccga ctcatccaga cacggaaccg ggaacgtggt
     1501 cctgtggcaa tctgatttgc gcgtctcctg gcgcgcacag tggctttcct tgctgattca
     1561 tgggctggtt gccgctgtta ttttactcat gccctggcca ctcagttaca ccccgttatg
     1621 gatggtgtta ctttcgctgg tggtgtttga ttgcgttcgc agccagcggc gtattaatgc
     1681 tcgccagggg gaaattcgct tgttgatgga cgggcgtttg cgttggcaag ggcaggagtg
     1741 gtgcatcgtc aaagcaccgt ggatgattaa gagcggcatg atgctgcgtt tacgttctga
     1801 tggcggtaaa cggcaacatt tatggctggc agccgacagc atggacgaag ccgaatggcg
     1861 ggatttacgg cggattttgt tgcaacaaga gacgcaaaga taaagatgac ggcaaagcat
     1921 atgcgctaag ccgtcagtga gatcaggcgt aatgctctgc catttcgttg aggatttgct
     1981 cgcaccagct ctgaatacgc tcgtcgctaa ggtcatactg gttagtttca tccagcgcca
     2041 gacccacgaa cagttgcccg tcagcaatca ccggtttcgg gctggtaaat tcatatcctt
     2101 ccgttggcca gtagccgacg aacttcacgc ctttggtcga gagtttgtca tgcagcatac
     2161 cgagcgcatc gaggaaccac tcgccgtatc ccagttgatc gccaagccca tacagcgcaa
     2221 caattttacc ttcaaggttc aggtcgtcga gctgatccca gacggcttcc cagtcttcct
     2281 ggatttcacc aaaatcccag gtcgggatac ccagaatgag cacatcgtac tgctccatta
     2341 atttcgggga gtcgtccttg aggttatgta aggtcaccag ttctgggccg ataatatcgc
     2401 ggattttttc tgccgccatt tcggtgtaac aggtgctgga accgtaaaaa agacccatat
     2461 tcataacgta agcttctcga ttctgctgtg gcttttgcag ctagtgtatc agaatcgctt
     2521 tagtcacggc acaataaggc ataatggatc aaatgagtga ccataagggg cgcaagtgaa
     2581 acaggatctg gcacgcatcg agcagtttct tgatgctctg tggctggaaa aaaatctggc
     2641 tgaaaatacg ttgaacgctt accgtcgcga tctgtcaatg atggtggagt ggttgcatca
     2701 ccgcgggttg acgctggcga cggcgcaaag tgacgatttg caggcattac tggcagaacg
     2761 gctggagggc gggtataaag ccaccagctc agcgcgtttg ctgagtgcag tgcgccgatt
     2821 gttccagtat ctttatcgcg aaaagtttcg tgaagacgat cccagtgcgc atctcgcttc
     2881 accgaaattg ccccagcgtt tgccaaaaga tttaagcgaa gcgcaggtcg aacgtttatt
     2941 acaggcacca ttaattgatc agccactgga gctacgcgat aaagccatgc ttgaagtgtt
     3001 gtatgctacc gggctgcgtg tctctgaact ggtcggactg acaatgagtg atatcagcct
     3061 gcgtcagggc gtagtgcggg tcattggtaa aggcaacaaa gagcgcctgg taccgttagg
     3121 tgaagaggcg gtttactggc tggaaaccta tctggaacat gggcgtccgt ggctgctgaa
     3181 tggtgtgtca attgacgtgt tgtttcccag ccagcgtgcg cagcagatga cgcgacagac
     3241 cttctggcac cgcattaaac attatgctgt gctggcgggt atcgacagcg aaaagctgtc
     3301 accgcatgta ttgcgtcacg cttttgccac ccatttatta aatcatggtg cggatttacg
     3361 cgtggtgcag atgctactgg gccacagcga cctctccacc acgcaaattt atacgcatgt
     3421 cgctaccgag cgtctgcggc aacttcatca acagcatcac ccgcgggcgt gatgtctgaa
     3481 aagaacggga agatttatga agaaaggttt tatgttgttt actttgttag cggcgttttc
     3541 aggttttgct caggctgatg acgcggcaat tcaacaaacg ttagccaaaa tgggcatcaa
     3601 aagcagcgat attcagcccg cgcccgtagc tggcatgaag acagttctga ctaacagcgg
     3661 cgtgttgtac atcaccgatg atggtaaaca tatcattcag gggccaatgt atgacgttag
     3721 cggcacggct ccggtcaatg tcaccaataa gatgctgtta aagcagttga atgcgcttga
     3781 aaaagagatg atcgtttata aagcgccgca ggaaaaacac gtcatcaccg tgtttactga
     3841 tattacctgt ggttactgcc acaaactgca tgagcaaatg gcagactata acgcgctggg
     3901 gatcaccgtg cgttatcttg ctttcccgcg ccaggggctg gacagcgatg cagagaaaga
     3961 aatgaaagct atctggtgtg cgaaagataa aaacaaagcg tttgatgatg tgatggcagg
     4021 taaaagtgcc gcaccagcca gctgcgacgt ggatattgcc gaccattacg cacttggcgt
     4081 ccagcttggc gttagcggta ctccggcagt tgtgctgagc aatggcacac ttgttccggg
     4141 ttaccagccg ccgaaagaga tgaaagaatt cctcgacgaa caccaaaaaa tgaccagcgg
     4201 taaataattc gcgtgaaaca acagatacaa cttcgtcgcc gtgaagtcga tgaaacggca
     4261 gacttgcccg ctgaattgcc tcccttgctg cgccgtttat acgccagccg gggcgtgcgc
     4321 agtgcgcaag aactggaacg cagtgttaaa ggtatgttgc cctggcagca actgagcggt
     4381 gtcgaaaagg ccgttgagat cctttacaac gcttttcgcg aaggaacgcg gattattgtg
     4441 gtcggtgatt tcgacgccga cggcgcgacc agcacggctc taagcgtgct ggcgatgcgc
     4501 tcgcttggtt gcagcaatat cgactacctg gtaccaaacc gtttcgaaga cggttacggc
     4561 ttaagcccgg aagtggtcga tcaggcccat gcccgtggcg cgcagttaat tgtcacggtg
     4621 gataacggta tttcctccca tgcgggggtt gtacacgctc gctcgttggg cattccggtt
     4681 attgttaccg atcaccattt gccgggcgaa acattacccg cagcggaagc gatcattaac
     4741 cctaacttgc gcgactgtaa tttcccgtcg aaatcactgg caggcgtggg tgtggcgttt
     4801 tatctgatgc tggcgctgcg cacctttttg cgcgatcagg gctggtttga tgagcgtggc
     4861 atcgcaattc ctaacctggc agaactgctg gatctggtcg cgctgggaac agtggcggac
     4921 gtcgtgccgc tggacgctaa taatcgcatt ctgacctggc aggggatgag tcgcatccgt
     4981 gccggaaagt gccgtccagg gattaaagcg ctgctggaag tggcaaaccg tgatgcacaa
     5041 aaactcgccg ccagcgattt aggttttgcg ctggggccac gtctcaatgc tgccggacga
     5101 ctggacgata tgtccgtcgg tgtggcgctc ttgctgtgcg acaacatcgg cgaagcgcgc
     5161 gtgctggcaa atgaactcga tgcgctaaac cagacgcgaa aagagatcga acaaggaatg
     5221 caagttgaag ccctgaccct gtgcgagaaa ctggagcgaa gtcgcgacac gctacccggc
     5281 gggctggcaa tgtatcaccc cgaatggcat cagggcgttg tcggtattct ggcttcgcgc
     5341 atcaaagagc gttttcaccg tccggttatc gcctttgcgc cagcaggtga tggtacgctg
     5401 aaaggttcag gtcgctccat tcaggggctg catatgcgtg atgcactgga gcgattagac
     5461 acactctacc ctggcatgat gctgaagttt ggcggtcatg cgatggcggc gggtttgtcg
     5521 ctggaagagg ataaattcga actctttcaa caacggtttg gcgaactggt tactgagtgg
     5581 ctggaccctt cgctattgca aggcgaagtg gtgtcagacg gtccgttaag cccggccgaa
     5641 atgaccatgg aagtggcgca gctgctgcgc gatgctggcc cgtgggggca gatgttcccg
     5701 gagccgctgt ttgacggtca tttccgtctg ctgcaacagc ggctggtggg cgaacgtcat
     5761 ttgaaggtga tggtcgaacc ggtcggcggc ggtccactgc tggatggtat tgcttttaat
     5821 gtcgataccg ctctctggcc ggataacggc gtgcgcgaag tgcaactggc ttacaagctc
     5881 gatatcaacg agtttcgcgg caaccgcagc ctgcaaatta tcatcgacaa tatctggcca
     5941 atttagcgtc atcttctcta taaaaaagag cgtggattgg gtacaatccc gctcttatca
     6001 ccgcattttg actagctcaa taaaagaaat cagaccatgt ttgaaattaa tccggtaaat
     6061 aatcgcattc aggacctcac ggaacgctcc gacgttctta gggggtatct ttgactacga
     6121 cgccaagaaa gagcgtctgg aagaagtaaa cgccgagctg gaacagccgg atgtctggaa
     6181 cgaacccgaa cgcgcacagg cgctgggtaa agagcgttcc tccctcgaag ccgttgtcga
     6241 caccctcgac caaatgaaac aggggctgga agatgtttct ggtctgctgg aactggctgt
     6301 agaagctgac gacgaagaaa cctttaacga agccgttgct gaactcgacg ccctggaaga
     6361 aaaactggcg cagcttgagt tccgccgtat gttctctggc gaatatgaca gcgccgactg
     6421 ctacctcgat attcaggcgg ggtctggcgg tacggaagca caggactggg cgagcatgct
     6481 tgagcgtatg tatctgcgct gggcagaatc gcgtggtttc aaaactgaaa tcatcgaaga
     6541 gtcggaaggt gaagtggcgg gtattaaatc cgtgacgatc aaaatctccg gcgattacgc
     6601 ttacggctgg ctgcgtacag aaactggcgt tcaccgcctg gtgcgtaaga gcccgtttga
     6661 ctccggcggt cgtcgccaca cgtcgttcag ctctgcgttt gtttacccgg aagttgatga
     6721 tgatattgat atcgaaatca acccggcgga tttgcgcatt gacgtttatc gcgcgtccgg
     6781 cgcgggcggt cagcacgtta accgtaccga atctgcggtg cgtattaccc acatcccgac
     6841 cgggatcgtg acccagtgcc agaacgaccg ttcccagcac aagaacaaag accaggccat
     6901 gaagcagatg aaagcgaagc tttatgaact ggagatgcag aagaaaaatg ccgagaaaca
     6961 ggcgatggaa gataacaaat ccgacatcgg ctggggcagc cagattcgtt cttatgtcct
     7021 tgatgactcc cgcattaaag atctgcgtac cggggtagaa acccgcaaca cgcaggccgt
     7081 gctggacggc agcctggatc aatttatcga agcaagtttg aaagcagggt tatgaggaac
     7141 caacatgtct gaacaacacg cacagggcgc tgacgcggta gtcgatctta acaatgaact
     7201 gaaaacgcgt cgtgagaagc tggcgaacct gcgcgagcag gggattgcct tcccgaacga
     7261 tttccgtcgc gatcatacct ctgaccaatt gcacgcagaa ttcgacggta aagagaacga
     7321 agaactggaa gcgctgaaca tcgaagtcgc cgttgctggc cgcatgatga cccgccgtat
     7381 tatgggtaaa gcgtctttcg ttaccctgca ggatgtgggt ggccgcattc agctgtacgt
     7441 tgcccgtgac gatctgccgg aaggcgttta caacgagcag ttcaaaaaat gggacctcgg
     7501 cgacatcctc ggcgcgaaag gtaaactgtt caaaaccaaa accggcgaac tgtctatcca
     7561 ctgtaccgag ctgcgtctgc tgaccaaagc actgcgtccg ctgccggata aattccacgg
     7621 cttgcaggat caggaagcgc gctatcgtca gcgttatctg gatctcatct ctaacgatga
     7681 atcccgtaac acctttaaag tacgctcgca gatcctctct ggtattcgcc agttcatggt
     7741 gaaccgcggc tttatggaag ttgaaacgcc gatgatgcag gtgataccag gcggtgccgc
     7801 tgcgcgtccg tttatcaccc accataacgc gctggatctc gacatgtacc tgcgtatcgc
     7861 gccggaactg tacctcaagc gtctggtggt cggtggcttc gagcgtgtat tcgaaatcaa
     7921 ccgtaacttc cgtaacgaag gtatttccgt acgtcataac ccagagttca ccatgatgga
     7981 actctacatg gcttacgcag attacaaaga tctgatcgag ctgaccgaat cgctgttccg
     8041 tactctggca caggatattc tcggtaagac ggaagtgacc tacggcgacg tgacgctgga
     8101 cttcggtaaa ccgttcgaaa aactgaccat gcgtgaagcg atcaagaaat atcgcccgga
     8161 aaccgacatg gcggatctgg ataacttcga ctctgcgaaa gcgattgctg aatctatcgg
     8221 tatccacgtt gagaagagct ggggtctggg ccgtatcgtt accgagatct tcgaagaagt
     8281 ggcagaagca catctgattc agccgacctt cattactgaa tatccggcag aagtttctcc
     8341 gctggcgcgt cgtaacgaca ttaacccgga aatcacagac cgctttgagt tcttcatcgg
     8401 tggtcgtgaa atcggtaacg gctttagcga gctgaatgac gcggaagatc aggcgcaacg
     8461 cttcctggat caggttgccg cgaaagacgc aggtgacgac gaagcgatgt tctacgatga
     8521 agattacgtt accgcactgg aacatggctt accgccgaca gcaggtctgg ggattggcat
     8581 cgaccgtatg gtaatgctgt tcaccaacag ccataccatc cgcgacgtta ttctgttccc
     8641 ggcgatgcgt ccggtaaaat aagcattacg ttatgctcac aaccccggca aatgtcgggg
     8701 ttttttaatt gtgctgcgcg aaagcagaca acaattttct tgcttcacta ttggctgcct
     8761 gcatcaccat ccacggactg aacgcccacg gtgtggcatc aataccgtgt aatacatctg
     8821 ctaaatcaca ccattgataa tccatcactt catcatcgtt gatctgtaac gcactggtgg
     8881 tgcgtgcggc aaataccgga cacacttcat tttccacaat gccattcgga tcggtggcgc
     8941 gatagcgaaa gtcaggatag atagattcag gaggcgtaat ttccacgcca agctcataac
     9001 ggcaacggcg gatcaccgcg tcttcgttgc tttctcccag ttgtgggtgc ccacaaaccg
     9061 agttagtcca cacgccaggc cacgcttttt tgctcagtgc gcggcgggta actaataatt
     9121 gtcctttggc attaaacagc caactggaga acgcgagatg taagcgggtg tctgccgtgt
     9181 gtgcggcata cttttccagc gtaccagtgg gaactccctg tgcattcaat aaaatgacgt
     9241 gttccgtttg cataatttct cacatgtaat tctgatcact catcattata gataattgaa
     9301 gagtgaaatg attaaccaag tgagcgttta tcgacaaccg cccgttttga gcggatgccg
     9361 acaggtaaaa accatttaaa aaagtagcat catatatttg aagcgacttt caatggccat
     9421 tgcaacacag tcagaaatga caggaataga gagagtaaaa agcaacagca gactgaatat
     9481 agcgcgcatt aaaaaattca tattattctc catgctcatt tttcttcagc ttgcgcatca
     9541 gaagccacat acgtactgtc ctgactttta aatctttatc agagacggat tccttcacca
     9601 ggtcagcatg atgctgatag ccattaaaga agacattcag cacaacggcg ctaagggttg
     9661 cgagcataat gccgctatgc agcaacggtt gcagtacggc cggtaactta gaaaagaaat
     9721 cgtgagagag cgtcggagtc atgccaacgc cgagactgat cgccacaata tagaggttgt
     9781 aacggttggt ggtgtagtta cagcgcgaca gaattcgaat ccctgtcgcc agtaccatgc
     9841 cgaacatcac cagcccagcg ccgcccagca caaattgcgg aatggaggct accagcaccg
     9901 ccatttttgg caccatgcca aacaggatta atataattcc cgaagaaata cacacccagc
     9961 ggctatgaac gcgcgtcacg ctaaccaggc caacgttttg agaaaatgac gtgtggggga
    10021 agctgttaaa cgtgccgcct atcattgtcc ctacgccatc gacacgcagc ccgcgaataa
    10081 tatcgtgtga agagagttta cgaccgacta tttcacccag tgccaggaac atccccatcg
    10141 actcgataaa cacgatgatt aacacggcag tcatggtcag aatggaaacg ggatcgaaaa
    10201 tcggcatacc aaacgacatc ggggtgacaa tcgcaaacca cgaggcatca tgtagcccgg
    10261 ataaattgac ttcattcatc atccacgaaa gtaaaaagcc aaatacaatc cccagtaata
    10321 cggcgacgtt ggacataaat cctttcgcat agcgagtaat gagcaagata aaaattaaga
    10381 cggcaaagga gatacctaaa taaacgggat taccatattg cggattccct ttacctccgg
    10441 cggcccagtc aatacccacc tgaatgatgc taagcccaat agaagtaata accacaccgg
    10501 taaccagtgg cgggaataaa ggcatcaagc gaccaataag tggcgctaat aatgtggtga
    10561 taaaacccgc ggcgatagtg gcaccaaata tccccagcag gccgatatcc gggttcatcc
    10621 caatggctat cattggtgtt acagcagcaa aggtcaccga cataatcacc ggcaggcgga
    10681 tccccataaa gcggccgata ccgatacatt gcaataatgt gacgatcccg cagcaaaaga
    10741 gatccgagct aatgagcatc gcaatagctt cttttgagag gcccagtcgg tcaccaatca
    10801 taagaggaac agcgactgca cctgcgtaca tgacaaggac gtgttgcaga ccgagtatga
    10861 tcagctttcc tggtgataat atgcggtcaa cctcatcagt tgggcggtct tgcccagaag
    10921 atgagggaag ttgggaatct atggcgctca tcagtgtttc tcctttcgcc cgccagagta
    10981 aaaagcaacc gtttacgtta atgaaactgt ctaaacacat gctcgtggag tgccagtttt
    11041 tgcgaggaag gggagttaga gacaggaatt gcggaggcaa tcacataatc gtgcttaaac
    11101 cacgaaaatg agaagagaac ctgacaatga aattatctgt aattttctca tattgatatt
    11161 tcattaatca ttctggcatg tcgcactctc gcatttaatc gtttttatct ggatagcgct
    11221 cttttggtcg gcaatgttga aataatgaaa gcggcataga ttgaggggca ataatttgaa
    11281 ctcagtcgca aaattataat taaaacggtc atgctaccta atatatgccc gtttattgca
    11341 tcgcgaaatt tgagaagaca taactcatta aatgaaaggg atgcaaatga ataagtttat
    11401 cgctgctgaa gctgcggaat gtataggctg tcatgcttgt gaaattgcct gtgcggtggc
    11461 acacaatcaa gaaaactggc cgctgagtca cagtgacttt cgaccgcgta tccacgttgt
    11521 agggaaaggc caggctgcga atccggtggc ctgccatcac tgcaacaatg ccccttgcgt
    11581 tacggcttgt ccggttaatg ctctgacttt ccagtccgat agcgtacaac tggacgagca
    11641 aaaatgtatt ggctgtaaaa gatgtgcaat cgcttgcccc tttggcgtcg ttgagatggt
    11701 cgatacgatt gcgcagaaat gcgacctttg taaccagcgc agttccggca cgcaagcctg
    11761 tattgaagtc tgcccaacac aggcgttacg actgatggac gataaagggt tacagtggat
    11821 aaaggtggct cgccagcgca aaacggcagc aggaaaagcg tcatcagatg ctcagccatc
    11881 tcgcagtgca gcgttgctcc ccgttaactc gcgtaaaggc gcagataaaa tttcagcgag
    11941 tgaacggaaa acccactttg gcgaaatata ttgcgggctg gatccactgc aagcgactta
    12001 tgagagtgac cgctgtgttt attgtgccga aaaagctaac tgcaactggc attgcccgct
    12061 gcataacgct attccggatt acatccgtct ggtacaggaa ggaaagatta ttgaagcggc
    12121 agaactttgc caccagacca gttccttacc agaaatttgc ggcagggtat gtccacagga
    12181 ccgtctttgt gaaggcgcat gtactttgaa agatcactcg ggcgcagtct ctatcggtaa
    12241 tctggaacgc tacatcaccg ataccgcgct ggcgatgggc tggcgtcccg atgtcagcaa
    12301 agttgttccc cgtagcgaaa aagtggcggt gattggcgct gggccagcag ggttagggtg
    12361 tgctgatatt ctggcgcgcg cgggagttca ggttgatgtc tttgatcgcc atccagaaat
    12421 tggcggtatg ctgacttttg gcattcctcc tttcaaactc gataaaacgg tattaagcca
    12481 gcggcgagag atattcaccg caatgggaat cgactttcat cttaactgtg aaattggccg
    12541 cgatattacc tttagcgatt taacttctga atatgatgca gttttcatcg gcgtggggac
    12601 ttacgggatg atgcgagcag atctgccgca tgaagatgcg cccggtgtca ttcaggctct
    12661 accgttcctg actgcccata cccgccagct catgggattg ccggagtctg aagagtatcc
    12721 gctgacggac gtggaaggta agcgagtcgt ggtattgggc ggtggcgata cgacaatgga
    12781 ttgtttgcgg acttccatcc gcctcaatgc cgccagcgtg acctgcgcgt atcgtcgtga
    12841 tgaagtcagt atgccgggct cgcgcaaaga ggtggttaat gcgcgcgagg aaggtgtcga
    12901 gtttcagttc aatgttcaac cgcaatatat cgcttgtgac gaagatgggc gtttaactgc
    12961 ggtgggcctg attcgtaccg ccatgggtga gccggggccg gatggtcgcc gtcgtcctcg
    13021 tccggtagcg ggttcagagt ttgaattgcc cgccgatgtt ctgattatgg cctttggttt
    13081 ccaggcgcat gccatgccgt ggttgcaggg cagcggaatt aaactcgata aatggggcct
    13141 gattcaaacc ggtgacgtcg ggtatttacc tacccagacg catctgaaaa aagtctttgc
    13201 tggtggtgat gcagttcatg gcgcggatct ggttgtcact gcaatggccg caggaaggca
    13261 ggcggcgcgc gatatgttaa ctctgtttga tacgaaggca tcgtgatgaa atcgttaatt
    13321 atcgttaatc cggctgactg tattggctgc cgtacttgtg aagtagcctg tgtggtcgct
    13381 cacccttcag aacaggagtt gaatgccgat gtctttttac cccggctgaa ggtacagcga
    13441 ctggacagca tcagtgcgcc ggtgatgtgc catcagtgtg aaaacgcccc ttgtgttggc
    13501 gcttgccccg tgggggcgct gacgatgggc gagcaggtgg tgcagaccaa ttctgcccgt
    13561 tgtattggct gtcagagttg cgtcagtgcg tgcccgtttg gaatgatcac cattcagtcg
    13621 ttgccggggg atacccgaca acaaattgtg aaatgcgatc tctgtgaaca gcgggaagag
    13681 gggccagcct gtgttgaatc ttgcccgaca caggcgctgc agttgcttac tgaaagagaa
    13741 ctcaggcgag tccgccagca gcgtattgtt gccagcggtg agaatcctct gtgaaagaaa
    13801 tctccgcgct gaaaggcgcg gagaatctat tagattgccc aaccacccgc gtaaaatgcg
    13861 accagtgcgg cagtaatgat caccgtacca atattcaact tttgccattc gcgagcaaag
    13921 acacggccta cgaccagtgt cacaaagccc agcataatac cggtaacgat attacaagtc
    13981 agaacgatga acacggcaca caccaggcca gccattgcgt caataaaatc gttgaaatcc
    14041 agcttcgaga cgttacttaa catcagcaaa cccacgtaca tcagagcggg tgcagtggcg
    14101 taaccaggga tcaaaaatga cagcggtgat aagaacagaa tcagcaggaa taacgccccc
    14161 actacggttg cggtcaaccc tgttttaccc ccggcggcgg ttcctgccgc tgattcgata
    14221 taaaccgctg cgggcgctgc gcccaccaga ccggagaata ttgaacttac tgagtcactg
    14281 gtcagggctt tgccgccatt gatgatctgg ttgtctttat ccaacaaatt cgcctgaccg
    14341 gcgacggcac ggatggtgcc agtggcgtcg aacactgcgg tcatcaccaa tgccagtaca
    14401 ctcggaagta cggttggctg gagtgcgccc ataatatcga ggctgaaaat cagagactta
    14461 ccatcttcgc cagtcagact tggcatcgcc accagaccgt ggtatttcac cgctgggtca
    14521 aagattaagc cgataatcga aattgcgata atcaccaaca agatcccgcc gggtacgcgg
    14581 cacttctcca gaccgaagat gaccgccagc cccagcaagc tcatcatcac cgggaaggag
    14641 gtaaacgcac cgagcgccac tggcaagcct tcaatcgggt ttttgataac catacccaca
    14701 ccgttagcag caatcagcag cagaaacagg ccgataccga tacctgtacc gtgagcgata
    14761 cccatcggta aattacgtaa gatccaggta cgcacaccgg ttacggaaat agcggtgaag
    14821 atgacgccca tcagaaagac cgcgcccagt gcgacaggaa cgctaatttg ttgcccgagt
    14881 accagactga atgcggtaaa tgccgtcaag gaaatcgcac aaccaatcgc cattggcaaa
    14941 ttggcccaca accccatcag caacgagccg aagcccgcga ccagacaggt ggcaacaaac
    15001 acagctgcgg gaggaaaacc tgctttgccc agcattcccg gaacgacgat aacggaataa
    15061 accatggcca gaaaggtcgt taagccagcc agtacttcct gacgaacggt actgccacga
    15121 gcggtaattt taaaataatt atccagcgcg ccctgtggct ttggtgcgtc cggtgtttgt
    15181 aggatgtctc cagacataat gatgtcctca cgaggatgtt aaagttttat tattagttgc
    15241 gttcgtacac cagacgacca tcaacgtagg tgcggtagat cgaacggtca tcgcccaacg
    15301 tcatcatcac gaacaatttg tcgactaaag aaacagagtt gtcatagcgc agctgctgta
    15361 gcggagtggc ggtgggttcc atcaccacga aatcagcctc tttgccaggt aaaaagttgc
    15421 caatcaaatc gtcaaggccc agagatttcg ctccgccgag cgtggccagg taaaacgctt
    15481 catatgccga gaggcgatag ccttgtaatt gcaatacttt gtaggcttcg ttcagcgttt
    15541 gcagcatgtt gaaagtggtt ccggcaccga tatccgttcc catgcccact ttaactttct
    15601 tctgccatgc ttttttcaag ttgaataagc cgctgccgag gtaaaggttg gaggtcggac
    15661 agaaagcaat gctggatttg gtttcgctga gacgatccca ctctttttct tcgagatgga
    15721 cgcagtgagc aaagacacag tttttaccgg tcaggccgta ctgatggtaa acatccagat
    15781 aaccatcatg gtcaggataa agcgatttca cccaggcaat ttcatctttg ttttcacaga
    15841 gatgggtatg tacccacgta tccggatatt cttctttcag gcgttgcgcc atcgccatct
    15901 gttcaggaga tgaggtcggg gcgaagcgtg gcgtaatcgc atatagcaga cgaccatttt
    15961 tgtgccagcg ttcaatcagt tctttgcttt ggtgatagct gctttcggca gtgtcgagca
    16021 gataatccgg tgcgttgcgg tccatcatca ccttaccggc aatcatacgc atattgatat
    16081 gactggcggc ttcaaacagc gcatcaacag attgcggatg aacagtgcca aacaccagcg
    16141 ccgtggtggt tccgttacgt aaaagctgct tgatgaagaa tgccgacatt tcgcgggcgt
    16201 actctaaatc ctcataacga cgttcagtag ggaaggtgtg tttattcaac cactccagca
    16261 attgttcacc ataggccccc accatttcac tttgcggata atggatatgt gtatcgacaa
    16321 agcccggtac tatcagtttg ccgcgatagt cgcgcacgcg aatagtgtca ggaatttgat
    16381 gctttccgtc ttcccattcg ccaaaccatt ccacttttcc ctgtttaatg agtaataaac
    16441 catcctcaat aaaccgcagc gcagaggcaa tctcttccgg gttatcgacc gtacgggtga
    16501 catcaataaa actgcctcgt accgctttca acgtgtgttc tcctgacatc attgactcct
    16561 ttactgttaa tccatctctt ccgctgaggt aataccaggc agaacgtttt cctgtcggta
    16621 gccaccaggg aggataatat ttaataaaat cgcagttaac ccgccagcac aaatagggtt
    16681 ttcaactagt acataaatag aggctggcaa tattttaaaa atttcgggat cgtaggagac
    16741 gccaagtcct aaacctaaag aagtggcgac aataagtgtt tctcggcgct ttaaaccgtt
    16801 ggtgatgatt atgcgaatcc cagcgatggc aatcatggaa aacatcaacg tcattgcgcc
    16861 tcccagaact gccgagggaa tggtcgtgaa gaagccgcca atcatcggaa acaaaccgag
    16921 gataaccagc attaccgcga tggttcgccc gacataacgt gaagcgacgc cagtcatctg
    16981 aataacccca ttattttgcg caaacgtggt taagggtaat gacccgacag cggaggcgat
    17041 aacagaaacc agaccgtctg ccagcacgcc gcctttcagt cgggactgat actcttctcc
    17101 ctgaatgggt cggcgggaaa ccattgccgt ggcggtgata tcgccgacag cttccagcac
    17161 gctaagcaga taaatcgtgc caaccaccag gaactgatgg aagctaaaac taaagccgta
    17221 tttgaacgga tgcgggatgg tgattaacgg caaattgcgc atactgctga aatccaccat
    17281 gcccaggcat aacgatgcga tatagccgac acatagccca atggcgatcc ctcccatgcg
    17341 tagcaacgga ctgcgacagc agttaaagcc gatcaccaca attaaaacca ataaaccaac
    17401 gccgagatgt tcgtaattgc cgaatgtacc gctgcttttg gctgcaaatc ctccaccaaa
    17461 atcgataatg ccgactttaa tcaggcttaa gccgatcatc agtaccacaa taccgctgac
    17521 ggtaggcgta ataacccgac gtaaataggg caagataaat gaagatccga caaccagaaa
    17581 tgcgccaacg aaggagacgc cgagaagcga cgacatgatt aactcttcgt gaaaaccgtc
    17641 gcttttcatg ctgctgccca gcgcaatcat gaccgtaaca aatgaaaaat tgactgactg
    17701 aattgagagt aggccagaac cgacgatgcc gtagcggttt acttgtaacc aggtaccaat
    17761 accagaggcg atcatcgcca tagaaacaag ataggcagtt gtttcagcgg aaagctgtaa
    17821 ggccgccccc acgattaacg cgggggttac catcggaacg aaaattgcca acagatgggt
    17881 aatggcacca acgagagcct gatgaaaggg agggcgatcc tccagttcaa atataaggtc
    17941 agaacctgca ttgtttatat cagacatccc ttcactcctt ttaaagtaaa tttgatcatc
    18001 tgaattagta aaataaattc ccacgaattt ggggcgcagg acgccttgag aacggcttat
    18061 ctcattttcg cagtcactat ttagtcatcg tccaaacaga gaaacaggcc gccgaagcgg
    18121 ccatttatta tttatatttt ttccagcgca gtgagtattt tctccggtgt gaaatgccat
    18181 tcgcgtaacc agatgccgca tgcatcgtga attgcggtag caatcgccgg agctgcgcca
    18241 tttacaccga tttccgagat cgatttcgcc ccgaacgggc cgactttatc gtcgctcggc
    18301 accagcacag cgcggaaatc acgcggaatg tcaccaattt tcggtgcgcc gtaactgcgt
    18361 aaatcacgcg ttaacgggtg accttcggcg tcataaatga tctcttcgct catactgtgg
    18421 ccgatagctc gcagggtagc accgtagatt tgtcccaatg ctaattccgg gttgaccggt
    18481 gtaccgcagt ccagcaaggc gtagaattta tccaggcgga tttcacccgt acgcgtgttg
    18541 acggcaactt cagcgaagtt tgcgccatac gggaaggcga aatcaggcgt gatataactg
    18601 ccagttccca ccagtgaacc aaagccggtg ccggtttcgc ctttatgagc aatatccccg
    18661 aagctgactt cgcctttctt gccgcgcacg acgcccggcg ttgccagttg aacatctgcc
    18721 actgcctcac ccaacatttg cgcgccgtgg aacagaattt tctcccgcag attttccgct
    18781 gccaaacgcg ccgcgttacc cgagaagcaa gtaccggacg aggcatatgc gcctttatca
    18841 aacaacgcat gatcggtatc accggagata acatgcacgt cctgcggtgg gcagtgcagc
    18901 acttctgctg ccagtttcgt cactacagta tccagaccag taccaatatc cgcaccgcca
    18961 gaatgaacga taaaggtgcc gtccgattcc agtttgatca tgcagttagc ctgatcgata
    19021 tccgggatac ccgatttctg catgataatg gcgacgccgc gaccgatgtg ccagtcacta
    19081 ttttgcggtt tcggtgaaga ccattggatc atctcgcgac cctgacgcag gatctcttcc
    19141 agtgcgcagc tggcggcgga aggaacggag gtcggcgctt taccttcacc gattgcaccg
    19201 agaattttca gctcttgccc ttcgtgtacc cggttacgtt cgataatttc cagttggtcg
    19261 atctgtaact gttcagccag ttccgctaat gccatggtga tagcgaagtt acctttcggt
    19321 gcgccataac cctgataagc accatttggg caaatgttgc tgtagtaggt ggtgacctgg
    19381 aaatcgacgt tatcgcacgg atataacggc agcgacagcg ccggtccgtt acacggtacg
    19441 gtgagtgagt ggttgccgta agggccagtg ttggcgcgga aatccatctt cactgccgtc
    19501 aggcgaccat cttttttcgc tcccagtttg acggtgactt tcgcgacgtg acgagaggtg
    19561 ttagcaataa actcttcttc acgggtgtag cggaacagta ccggacgccc ggtcacgcag
    19621 gttgcccatg cgcacacttc ttccagcagg atgtcctgtt tggagccaaa accgccgcca
    19681 actcgctctt taatgacatg aactttatgc tgtttcatgc ctacgaggcg cgcgacctgg
    19741 cggcgtaagt gccatggtac ctgggtggag gcgtggataa ccagacgatc gccgtccatc
    19801 cgggtaaagc agatatgtgt ttcagtcggg cactgctgtg cctgcgttga gttataggtt
    19861 cgctcaatga tcacatcggc atcggcaaag cctttgtcca tatcgccaat atgaccatga
    19921 atactggcgg cgatattttt gcgagggcga gaaccgatcg ggaagttgat gatcatatgc
    19981 tcgccgcgct gggctgcatg gctgttatcg tcttccagag tatctggcgc accagcaaca
    20041 tacaccaccg gttcatcgtg cacgacaggc gcatcttccg ccattgcttc gtcgatcgac
    20101 attaccggct taagcacttc atattcaacg tcgatgagct tcaatgcttc gagcgcaatt
    20161 tcttcacttt ctgcgacgac cgcagcaaca cgatcgccga cgtgacgcat tttcttgccg
    20221 aacatacggc ggtcaagcgg tgacggttcc ggtgcgctct gaccacccgg ggtatagtag
    20281 atatccgggc aattcaggtg agtaataacg tgaacgacgc ccggtaaggc ttcagctttg
    20341 ctgacatcca gatgagtaat cagtgcgtga gcgtgtgggc tacgtaacat tttaatgacg
    20401 caggcgtcag ccgttacgcg gtcttcaaca tagcagggtt tcgcctgcac cattttcgcg
    20461 gcatcagttt taggataatg cttgccaatg acttcaaggt cgtcacggaa agtcggagcg
    20521 atatcaatgg tggcctgcgg attattttta cgtgcaaccg ccagttcaat gacctgatag
    20581 tattgttgcc agcctgcatc gcggctgaac aaaccagaaa gcgcgtcgtc gatctcttcg
    20641 cgggtaggtg cggcgatgcg atcgagaaga tcggtgataa tcagagctgc agctggatcg
    20701 ttataaccag actgcaccac gccaacatca accattgcct gttgaaccag acttaactcg
    20761 ttccatttgc ccaaagattc tgcggtacga atatctgcct tctctaactg tgcggcaata
    20821 agcaaggacg cgttaacgat attaccgtta aagattattg cgtcagaacc ggcaaacccg
    20881 aagccatcat cactgttgcg tacagagtgc attcccatgt taaacaacag cttttgcacg
    20941 ttttcgcctg gattaacggt tagctcctga ggcgcgccat ttaaagtaaa gtggatgatc
    21001 atacggcttc ctccccagcc tgttggcagt cggcatacag gtcggctacc agaactcccg
    21061 tgatatagcg tttataggcc acgctgcccc gcaaatcttc ctgcgggaaa atggcgttgg
    21121 cgacagcctg ttcaagtgca ttgccttcca gattttgttt ttcgacatca tgcagacgca
    21181 gtgctttact ggcgacgcca tccagcgcaa ttcgcatacc gtcgtggtct gtcattgcaa
    21241 cggctgccgt cacgacggtt aaacctgcct gagaacggct aattttgcgg gtcgcacagg
    21301 tgcgatacgg atctttaatg ataatttcgg ttagcaggcg atcgcatggg caggccaggt
    21361 agtcctcgat tgacagcgtt tcgccgttgc caaaaaccag ttcagcatcc agtgccaaca
    21421 ggacgggaag cagcaccgac tcttcctggc gggcggcgat ttcaccacca atggtcgact
    21481 gattacgaac atggcgtgag taaacaaaac cgagggcttc acgcagcgct gcaggaataa
    21541 atcgcgcatc acgcagtggc tgcaagcgag acattgcgcc aatccgcagt gcaccgttat
    21601 cccagtcaac ccagtccagt tccagatcct gtaaggaaat ggcaatcttt ttatcggtac
    21661 gggttggtgt agcattgagt ttgctgcccc cggcgaacca gacggcttca tcctggtagc
    21721 ggcgcttcag ttccagcgcc tgttcgacag agtcgggcct gaaaaattgt tcaatcatct
    21781 tctttcctta tttaaccctc ttccctgcgg gaagagggca tctgtcattt atgccagcgc
    21841 atccatccga cgccacatac tggcagcggc ttttctggct tgtgcataaa tggaatcgca
    21901 atcgaagtta aactgacggt cttcatagac catcacacca ttgaccatca cgctgtgaac
    21961 gctgcctgag cccataccga aagcgatatg cccggcgata ttgtctgcca gcagcggcgt
    22021 cggtgagttg taatcgcaaa tggttaaatc agctttgtaa ccggcttcca gaagtccaaa
    22081 tttcgcgcca aagttgcggc tcatcagttc gttaccgtta gtcagggctt tggcaaaact
    22141 gtcaggccac agcggaccac ccgcatcgcg atgtttaaag aaggcaaatt tcatctcttc
    22201 aaacatgtcc gaaccaatgc cgtccgttcc caacgccaga ttgcggatgt cgctaaggtg
    22261 atggttgtag ccgacatggt tgttcatgtt tgaacgggcg ttatgcacca ggaacgcatc
    22321 gcgctgattg agtagggcga tgtcatcttt cgacaagtac agcccatgag cgaccagcgt
    22381 tttgctatcg atgagatcga attgcgccag tcgtgccagc aggtctttgc cgtaccagtg
    22441 gtgactgtag gaaacgtcgt aaaggtcttc cgcagcgtga atatgcaaac cacggcctgt
    22501 ggctttcacg gcttcatgta gcatctccag accggcatct ggcacggtaa acggcgcgtg
    22561 agcaccgata tgtgcttcca ccagatacgg ctcggttgct gctttcttcg cctcatcaat
    22621 ctgacgggcg aagcggatgt tttcttctac accttcctgc aactctttga tgccgttgtt
    22681 acggtcagta gtttcaaaac aggtcatcgc gcgcaggcca acttttaaaa atgcgtcgcg
    22741 caatgtggag agcgacccgc cgatatacgc cggagaggca tggtgatcga taaccgatgt
    22801 acatccgctc ttaatcgctt ccagggaaca aatcagcccg ctgtaataga gcgactcttc
    22861 atcaagggcg cgatcgagcc gccaccagag atttttcagc gttgagatga aatccgggca
    22921 gggggcgatg tttgccataa ttccgcggga aagccccgag taaaaatggt tgtgcgagca
    22981 gacaattccc ggcatcacaa tccgaccatg catctctttg tagctggcat cggggtagcg
    23041 ttgcgtcagg gcatcgccga tagcgacaat cacatcattt tcgatagcga tatcaacgcc
    23101 ttcctgcact ttcgccgggt gtaactgcac tgcggtgaca ttcttcagaa tcaacatgat
    23161 tacacctcca cgcggcccag cagataatga tgatgctggt ggacatggct gatgatgcgg
    23221 cacatatcgt tcagctccgg tggtacgttg ttgaactgac cttcgctgtc gatgtttaac
    23281 acccagcttt ggttattcag tcgtactcgt acccggcaat cttccacaag gaagcctggg
    23341 ttgctgctgt tatcaaagtc ttgcgccagg ctgaaaacgg tgattttgtc tttgtatggt
    23401 ttaccgttcc acggacagaa ctgagcgcag ttgccgcatt cgttacagta agcgtcgagg
    23461 tgcagcgtct ggaatctatt ctggaagcct gggaccgcaa tggagacgtt ggcgcggttc
    23521 gggcagacat ccacacactt gctgcaaacg tagttacatt cgaggcagcg agcggcttcc
    23581 tgggcgacaa acgcgtcacg atcgtcactg ttcaccagag tgatagagat atcgccttta
    23641 cgttgataga tttccgccgg attgacgttg ttccagtatt tatcgttctg gtgggaacgg
    23701 atattttccc gactaaggat ggcatcggtc gcccgacgcg cggttccgac agcagcgaca
    23761 atggaggatg gtccgcgctg tacgtcgccg atcataaaga cgtcagtcag acgagtttcg
    23821 ccattgtggt cgacgtctgg ccagccgttt ttgtccagcg gcacgcccat cgcattcagg
    23881 gcttcagtat cctgctgttc accaatggcg gtgatcaggc tgtctacatg cagtgttact
    23941 gtttcgttgg tttcaaccgg acggcgacgg cctttctcat cgggttcgcc aagcgacata
    24001 acgcgcaagg ttaaggtgcc atcagcatcg aaacgttccg gattattcag gaaacggaac
    24061 tccacgccgt cgtgcaacgc ttcttcatac tcttcgcgcc atgcgggcat ctcttgcagt
    24121 gaacgacggt aaacgatcgt tgctttttct acgcctggaa cgcgtaacgc cgcacgagcg
    24181 cagtccattg cggtgttacc cgccccgaca acgaccacat gtttgcccag cttgagcgct
    24241 gtacccttgt tgtattcacg caggaagggg agtgatttcc aaacattttg gttgtcaccc
    24301 gccagtttca caccgctatt tttatcagtg ccggtggcaa tcagaacata gtggaagccc
    24361 tgatttttta actgttcaac ggtcaaatcg ggtgagcagc catactcaaa tttcacgccg
    24421 tgatcggcaa caaaatcgat atcgtgctga attaactcag ccggaattcg gaactgagga
    24481 atgatatttt tcaccacgcc gcccgcattg gcttcgcgtt caaacagcgt aaccggatgg
    24541 cccgctctgg caaggaagta acctgctgcc agacccgccg gacctgcacc aatcacggca
    24601 accggatggc gtgaaccaga accggctggt ttgtgccagc gttgcttata ttcatcccaa
    24661 cctttttcca gcgcgacttt tttcagttcg cggatattca gcgcactgtc gtaatccagg
    24721 cgggtacagt tgtattggca ctggtgatcg caaatatgac cggtaatggc gggcagagcg
    24781 ttgcgttggt agatgagttc cagcgcgtcg gcatagcggt gttcgccaag cagacggatg
    24841 tattccggaa tatcttgctt gatagcgcag gcagtaacac agggggcaac gtagcaatcg
    24901 gtcagcggca ggtcttctgc cacttcaata cgctcttctg gcttccagtg tttctgggtg
    24961 tattccatgg ttaacgcatc tgctgccagt ctgttcagtc gttcgacgtc aacatggtca
    25021 agtccccagg cgtcggagcc ttccagctcg cgcatgcagg cacttaagcg caaatagccg
    25081 ccaggtttca gcaggtcggt tgccatagta atagggcgaa tacctgtatc aaaaatatcg
    25141 cggatagtca gctgactggc accaccggaa taagaaatgg gcagtttgcc gtcaaaggcg
    25201 cgagagagaa ctgctgcaac attgatggag agcgggaaca gcgcacggcc tgacatatac
    25261 atctcttcac caggcagtgc gcctttattg ttgatggtgc cgagagtgtt agtcagtttt
    25321 acgccaaagc cgagtgattt ttcttttgcc agtgccatca ggcgttccag catttccagt
    25381 gcttgcgtca gcttgaggtc gtgatcaaat gactcttctt ttaagcctat gtaaccgaaa
    25441 ccgcagacat cgaggatctc acgaacacgc gcgtatccca gtaaggtcgg gttaagtttc
    25501 acaaaggtgt tgagcccttt ttcttccagc atgtagcggc aaatggcttc gatttcatgc
    25561 ggaggacagc cgtgcatggt ggagagggtg acgccctgca ccatactggt ggggatgcga
    25621 gcgggtaagg cttgcaagct ttcgcgtttt tcctgcaatc cgtggcgggc taaaaatgcg
    25681 tcatcctgga gtaatttatt cagcgtatcg cgatattgcg cgaatttcgg atggtcagat
    25741 gcgtccatca tattgtcgat gaactgttgc attggcggtt gcttaatacc ttcgaggttg
    25801 taaccgacgc tcatattaaa gatgaacgat ttaccggaat cagaaggctg gaacatcgct
    25861 tcgagaaggt gcagggcaaa ccaggctttg aggtattcat cccaggcttt aagcagggta
    25921 aattcggtag accattcggt gttaaagcac tcgtcttcgg catcgataca gggcttttcc
    25981 agctccaggc ggtcaagaat ttggacggtt tttagttcga tgaagcgtcc gccagtcagc
    26041 caggaagtga caatattttg cgcgagctgc gtgtgcgggc cagcggcagg gccgacggga
    26101 gtggcacagg tttcaccgaa gacgctaaca gttttacctt ttacaggtga gtaaaactgt
    26161 tgctcgggaa taccaaagat tgagcgttgt tgttggtatt catcaaatat gcgcgtcaaa
    26221 agttcctcaa acggaatggg acgcataata tcccccataa ccctctcctt agcttataca
    26281 gatatcattg agtgaccggt gttgagaaat ccggtggttt agggggatag agcaacaatc
    26341 gcaccactct tgccaggttt ggcgggaaat ataaaaaaat gtgaggcggc tcgaaactta
    26401 aaggcttttt gtgaatgaac tcacaggtgc caaatgagaa ttaaaatcac tcctgaaaat
    26461 tattattgtc agcccattta tcactttttc tctttt
//
LOCUS       DAZFXT010000061        26060 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.061, whole genome shotgun sequence.
ACCESSION   DAZFXT010000061 DAZFXT010000000
VERSION     DAZFXT010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26060
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.061"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="ACHKW0_002727"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="ACHKW0_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HFO7475533.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="ACHKW0_002728"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="ACHKW0_002728"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HFO7475534.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="ACHKW0_002729"
     CDS             complement(941..1408)
                     /locus_tag="ACHKW0_002729"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO7475535.1"
                     /translation="MDANLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKW0_002730"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="ACHKW0_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HFO7475536.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKW0_002731"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="ACHKW0_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HFO7475537.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKW0_002732"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="ACHKW0_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HFO7475538.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKW0_002733"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="ACHKW0_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HFO7475539.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKW0_002734"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="ACHKW0_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HFO7475540.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKW0_002735"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="ACHKW0_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HFO7475541.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKW0_002736"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="ACHKW0_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HFO7475542.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKW0_002737"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="ACHKW0_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HFO7475543.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKW0_002738"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="ACHKW0_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HFO7475544.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKW0_002739"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="ACHKW0_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HFO7475545.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKW0_002740"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="ACHKW0_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HFO7475546.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKW0_002741"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="ACHKW0_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HFO7475547.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKW0_002742"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="ACHKW0_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HFO7475548.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKW0_002743"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="ACHKW0_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HFO7475549.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKW0_002744"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="ACHKW0_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HFO7475550.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKW0_002745"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="ACHKW0_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HFO7475551.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKW0_002746"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="ACHKW0_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HFO7475552.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKW0_002747"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="ACHKW0_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HFO7475553.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKW0_002748"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="ACHKW0_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HFO7475554.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKW0_002749"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="ACHKW0_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HFO7475555.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKW0_002750"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="ACHKW0_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HFO7475556.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="ACHKW0_002751"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="ACHKW0_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HFO7475557.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKW0_002752"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="ACHKW0_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HFO7475558.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKW0_002753"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="ACHKW0_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HFO7475559.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKW0_002754"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="ACHKW0_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HFO7475560.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKW0_002755"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="ACHKW0_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HFO7475561.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKW0_002756"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="ACHKW0_002756"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HFO7475562.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKW0_002757"
     CDS             15058..15441
                     /gene="rplQ"
                     /locus_tag="ACHKW0_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HFO7475563.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15548..15916
                     /locus_tag="ACHKW0_002758"
     CDS             15548..15916
                     /locus_tag="ACHKW0_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HFO7475564.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKW0_002759"
     CDS             15927..16352
                     /gene="zntR"
                     /locus_tag="ACHKW0_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HFO7475565.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKW0_002760"
     CDS             16408..16626
                     /gene="arfA"
                     /locus_tag="ACHKW0_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HFO7475566.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKW0_002761"
     CDS             complement(16623..17033)
                     /gene="mscL"
                     /locus_tag="ACHKW0_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HFO7475567.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKW0_002762"
     CDS             complement(17163..18539)
                     /gene="trkA"
                     /locus_tag="ACHKW0_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HFO7475568.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKW0_002763"
     CDS             complement(18561..19850)
                     /gene="rsmB"
                     /locus_tag="ACHKW0_002763"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HFO7475569.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKW0_002764"
     CDS             complement(19896..20843)
                     /gene="fmt"
                     /locus_tag="ACHKW0_002764"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HFO7475570.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKW0_002765"
     CDS             complement(20858..21367)
                     /gene="def"
                     /locus_tag="ACHKW0_002765"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HFO7475571.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKW0_002766"
     CDS             21497..22621
                     /gene="dprA"
                     /locus_tag="ACHKW0_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HFO7475572.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22593..23066
                     /gene="smg"
                     /locus_tag="ACHKW0_002767"
     CDS             22593..23066
                     /gene="smg"
                     /locus_tag="ACHKW0_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 family protein Smg"
                     /protein_id="HFO7475573.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23095..23637
                     /locus_tag="ACHKW0_002768"
     CDS             23095..23637
                     /locus_tag="ACHKW0_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HFO7475574.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGENLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHKW0_002769"
     CDS             23642..24214
                     /gene="tsaC"
                     /locus_tag="ACHKW0_002769"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HFO7475575.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24219..25037
                     /gene="aroE"
                     /locus_tag="ACHKW0_002770"
     CDS             24219..25037
                     /gene="aroE"
                     /locus_tag="ACHKW0_002770"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HFO7475576.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFSAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLQGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25034..25291
                     /locus_tag="ACHKW0_002771"
     CDS             25034..25291
                     /locus_tag="ACHKW0_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HFO7475577.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25267..25821)
                     /locus_tag="ACHKW0_002772"
     CDS             complement(25267..25821)
                     /locus_tag="ACHKW0_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HFO7475578.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         6697 a         6180 c         6753 g         6430 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgcgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagacc acgcttattt tttgacatcc
      421 cgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atccgacctg cgattagaac tggaaggcgc gaaggatctc cgtgaggcta
      781 tcgcgtatgc cgacagcgtt catgactatg ttagccgtga tatgatgatc gagatccttg
      841 ccgatgaaga aggtcatatt gactggctcg aaactgagct ggatctcatc ggtaaaatcg
      901 gtttacaaaa ttacctgcaa tcacaaataa aagttaaaga ttaacggcct gccagcaaac
      961 tatcccaacc tacaacgaaa cccgcagccg ccagaaatgg cccaaacggc agcgggtttt
     1021 ttaatgactc ttttcctctc atgagcagac caacaactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggcaagaagg tccaggtatg ccaggcacca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgacg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaataa tcgcccccca taacgcatcg gctaaaccgt
     1261 caggattaca aacctgagag aacaaaagac ctgaccagag taacgggcag gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccata aaaaaagtag gacagataaa caagcgtaca
     1381 aaatcagaaa gggaaggtta gcgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagaa atcgctgatt tgttaacctg gttccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatac agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctgaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcacaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctatcgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtact ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcctgg gtattgtaaa
     5221 accatggaac tctacctggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcgtt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggtaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattgttgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttataaa ggcaagggtg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactctg caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcctgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaaatctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctgtctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccaggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag attactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtatttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cctggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtgcgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaatattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaaaccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacctg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtc gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgcct ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcgtattct gaagtgtggc
    15361 ttccgtgcag gcgacaacgc gccgatggct tacatcgagc tggttgatcg ttcagagaaa
    15421 gcagaagctg ctgcagagta atctgaagca acgtaaaaaa acccgccccg gcgggttttt
    15481 ttatacccgt agcatcccca cttatctaca atagctgtac tctttttgtt catcccctgg
    15541 agtatatatg tggttacttg accagtgggc agagcgtcat atagcagaag cgcaagcgaa
    15601 aggtgagttt gataacctgg caggtagcgg cgaaccattg atactggatg atgattctca
    15661 cgtgccaccg gaattacgtg cggggtatcg cttgttgaag aatgccggtt gcttaccgcc
    15721 agaacttgag caacggaggg aagcaattca gcttctggat attctcaaag gtatccgtca
    15781 cgatgatccg caatatcaag aggttagccg tcgattgtca ttactggaat tgaagctgcg
    15841 acaagctgga ctgagtaccg attttttacg cggcgattat gctgacaagt tgttgaataa
    15901 aatcaacgat aactagtgga gtatgtatgt atcgcattgg tgagctggca aaaatggcgg
    15961 aagtaacacc cgacacgatt cgttattacg aaaaacagca gatgatggag catgaagtgc
    16021 gtactgaagg tgggtttcgc ctgtataccg aaagcgatct ccagcgattg aaatttatcc
    16081 gccatgccag acaactaggt ttcagtctgg agtcgatccg cgagttgctg tcgatccgta
    16141 tcgatcctga acaccatacc tgtcaggagt caaaaggcat tgtgcaggaa agattgcagg
    16201 aagtcgaagc acggatagcc gagttgcaga gtatgcagcg ttccttgcaa cgccttaacg
    16261 atgcctgttg tgggaccgcc catagcagtg tttattgctc gattcttgaa gctcttgaac
    16321 aaggggcgag tggcgttaag agtggctgtt gattttttgc actgacagga ttacactcgc
    16381 gccgttaaat aaccaactgg agtttttatg agccgatatc agcatactaa agggcagata
    16441 aaggataatg cgatagaagc attactacat gatcccttat tccgacagcg agtagagaaa
    16501 aataagaaag ggaaaggcag ttacatgcga aaaggaaaac atggcaatcg ggggaactgg
    16561 gaggccagtg gcaagaaagt aaatcacttt tttaccactg gccttctgct ttcaggcgtt
    16621 tgttaagagc ggttattctg ctctttcagc aaatcacgaa tttctgtcag taatacttct
    16681 tctttagttg gtgctggtgc ggctgctggt tcttcttttt tccgattcag tttgttgatt
    16741 agcttaatcg ccataaagat ggcaaaggcc acaatcagaa aatcaaagac gttttgaatg
    16801 aagacaccgt aatgcatcac aacagcaggg atatccccct gcgcatcgcg tagcgtgaca
    16861 gcaaactgtt taaagtcgat cccgccaatt aataagccca gaggtggcat gatgatatcg
    16921 gcaaccagtg aagatacaat cttcccgaat gccgcaccga taatgacacc caccgccaaa
    16981 tccaccacgt tcccgcgcat cgcaaattcg cgaaattctt taataatgct catgttattc
    17041 tccctatgaa gccgacaacc ataagtctaa caaatgttaa gccattttcc tgctaggaat
    17101 gaatttaatt aatccttgat aatcaaaggg aaataataag gcgtcattag acgccttatt
    17161 aattacaaga agaaagggct tggctggaag agtctttcga cgtcggtaat aaacttttta
    17221 tctgtgagga acataattac gtgatcgcct tgctcaatgc gcagattgtc attggcaatc
    17281 atcacgtcgt ttccacgtac caccgctcca ataatcgttc ctggcggtag cttgatttcg
    17341 tcaatgactc tgccgacaac gcgtgaggtg ctttcatcac cgtgagcaac ggcttcaata
    17401 gcttctgcta cgccgcggcg caatgaggaa acaccaacaa tatctgcttt tcgcacatgg
    17461 ctaagcaacg cagaaatagt tgcttgttgt ggtgaaatcg caatatcgat cacgctcccc
    17521 tgaaccagat ccacataagc gcgacgctgg atcaatacca tcaccttttt cgcacccata
    17581 cgtttggcaa gcatggcgga catgatattg gcctcgtcat cgttggtgac ggcaataaac
    17641 agatcaactt gatcgatatg ttcttcggcc agcagttctt gatccgacgc atcaccaaaa
    17701 aagacgatcg tattctgcaa cttttccgcc agttcggcag cgcgctgctg attacgttcg
    17761 atgagtttaa cgctgtaatc tttttccaga cgacgcgcca gccctgcacc gatattaccg
    17821 ccaccaacca gcatgatccg cttatacggt ttttccagtc gctgtaattc actcatcacc
    17881 gcacggatat gctgtgaagc ggcaataaag aacacttcat caccagcttc cacaatggtc
    17941 gaaccttgtg gacgaatggg gcgatcgtgg cggaaaattg ctgccacacg agtatcgata
    18001 tgtggcatat gttcgcgcat ggtcgaaagt gcgttaccaa tcagcgggcc gccataatag
    18061 gctttaacca cagcgaggct gactttaccc tcagcgaagt tcaccacctg caatgcgccg
    18121 ggatactcaa tcagtcggta aatattatcg ataaccaact gctctggtgc gatcagatga
    18181 tcaatcggta cagcatctga atgaaatagc ttatcggcat cgcgcacgta gtctggtgag
    18241 cggatgcgag cgatgcgatt aggggtgttg aaaagtgagt aggctacctg gcaggcaacc
    18301 atattggttt catctgaact ggttacagca accagcatat cggcgtcgtc ggcacctgcc
    18361 tcccgcaata cgcgtggatg agagccatgc ccctgcacga ctcgcaggtc aaatttatcc
    18421 tgcaaggtcc gcagacgctc accgttggta tcgacaacag tgatatcgtt gttctcgcca
    18481 accaggtttt ccgccagtgt gccgccaacc tggccggcac ccagaatgat aattttcatc
    18541 agtcgcgacc cgttatctca tcactttttg attagcttag cgtaaaagaa gccgtcgccc
    18601 tcttcggcac caggcagatt ttgtttaccc ggttgctctg gtgttcctgt ttcgcaaagt
    18661 tcggcatcag cggtacgttg caaaaaggct ttaatctgca ggctattctc ttccggtaac
    18721 accgaacagg tggcatagac cagggttcca ccggatttta aatgcggcca aatggcgtcg
    18781 agaatttcag actgcaattg cgcgagttcc gggatatcgc gatcgcgacg taaccattta
    18841 atatccggat ggcgacgaat cacaccggtt gctgaacaag gcgcatctaa taaaatgcga
    18901 tcaaactgtt gctcgccaca ccattgggaa gggtagcggc catcaccttg tttcacggtc
    18961 gctttcatac caaggcgttt taaattgtcg taaacgcgag agaggcgctg ttcgtcaata
    19021 tcaaccgcaa caacctgcgc ttctggtgcc acctcaagga tatgcgttgt tttaccgccg
    19081 ggggcggcac aaagatccag aatgtgttca ccgttttgtg gcgcaagcca ggtcatgcaa
    19141 ccttgtgctg atgcatcctg aacggtaacc catccgtctt caaaaccagg tagcgcatga
    19201 acaggtgcag gtgtttccag ccgtacagca tcagggtaat ccgcatgcgg gaaacctttc
    19261 attcctgctt catccaacaa tgcaagccag ctgtcgcggg aatgatgcgt acgattaacg
    19321 cgcagccaca ttggcggacg ctggttattg gcttcgacga tggattgcca ctgctctgga
    19381 tacgcttttt gcagacgctt cagcaaccag gaaggatgca gataacgtgc atcactggca
    19441 ttaaactcgg ctaataactc ttcttgctga cgctggaact ggcgtaatac accgtttatc
    19501 aaccctttaa gttgcggacg cttaattgcg atagcgcctt caaccgtttc agccagcgca
    19561 gcatgaggtg gaatgcgggt ataaagcagt tgatacaaac caaccataat caggtaatgc
    19621 acggtccgct gtttgccggt catcggacgg gccattaact tattaatcag ccagtctaac
    19681 tgcgaaagcg tacgcagtac gccaaagcac aactcttgaa gaagtgcttt gtctttatcg
    19741 gaaacttttt gctggagcgg tggcagaatg ttgcttaatg attgcccttg ctcgacgact
    19801 tgttcaacgg cctgggccgc catgctacgt aaattacgtt gttttttcat aagtataaaa
    19861 acgcccggca agaccgggct tagaagagtg gactatcaga ccagacggtt gcccggaaca
    19921 aaccattccc gacgagagtt caggaggtct tgcgcgctca tcgctttctt acccgcaggt
    19981 tgtaacgaga gcaggttcag gatgccatca ccagtcgcaa cctgaatgcc ttgtttgttg
    20041 gcttcaagga tcgttcctgg tgcagcgttg gttgccgtat caatgaccga tgctttccag
    20101 actttaaccg gctgtccttc aatttccagc cagctcattg gccatggatt gaaagcgcga
    20161 atgcagcgtt caagctgtgc tgccgaaagt gaccagtcaa tacgcgcttc ttctttactc
    20221 aacttttcgg cgtaagtgac aagagtttcg tcctgaactt ctggtttcgc cgtgccgtct
    20281 gccagttgtt tcaacgtggt gataagccct tgtgggccaa gctctgccag cttgtcgtac
    20341 agcgtaccac tggtatcttc tgcagtaatc gggcaggaga gcttatagag catgtcaccg
    20401 gtgtctaaac cgacatccat ttgcataatg gtcacaccag tttctgcatc acccgcccat
    20461 agtgagcgtt ggattggtgc agcaccgcgc cagcgtggca gcagtgaacc atgaacgttg
    20521 atacagccaa gacgcggcat ctccagcact gctttaggca gaattaagcc ataggcgacg
    20581 acgaccataa catcagcctg cagatcggcg accagttgct ggttttcttg tggacgcagg
    20641 gaaacaggtt gaaaaacggg cagacctttt tcctcagcca gaactttaac cgggctgggc
    20701 atcagttttt taccgcgtcc tgccggtcgg tctggctggg tgaacacgcc aacgacgtta
    20761 tgaccagaag acaacagcgc gtcgagatga cgcgctgcaa agtcaggtgt acccgcaaaa
    20821 ataatacgta gtgattctga cacgttagtt cttatcctta agcccgggct ttcagacgat
    20881 ccagtttttc aactttctga cgaatacgtt gttgtttcaa cggtgacaga taatccataa
    20941 acagtttgcc gaccaggtga tccatctcat gctgaataca gatggctaac agaccgtctg
    21001 cttccaattc aaatggttta ccgtcgcggt ccagggcgcg aattttaact ttctctgcgc
    21061 gtggcactaa agcacgttgt tcagggatcg acaggcaacc ttcttcaatg cctgtttcgc
    21121 cgcttttttc taaaagctcc ggattgatta acaccagccg ttcgtcacgg ttttccgaaa
    21181 catcaataac aatgatacgt tgatggatat caacctgggt tgccgccagg ccaatacctt
    21241 cttctgcgta catcgtctcg aacatatcat cgacgatacg ctgaatttct gcattcactt
    21301 cttctaccgg tttagcaact ttgcgaagcc gctcgtccgg aatatgtaac acttgcaaaa
    21361 ctgacataaa tctccagaga tgtgttcagg agttagaaag attatttctt ctattctaga
    21421 caaatccccc tctgattgac agcatcactg accaatcgca aagattgcta aggctgctta
    21481 tggcagggag ataaggatgg tcgatacaga aatttggctg cgtttaatga gtatcagcag
    21541 cttgtacggc gatgatatgg tccgtatagc tcactggctg gcaaaacagt cgcaaattga
    21601 tgcggttgga ttgcagcaaa cagggcttac attgcggcag gcacaacgct ttctttcatt
    21661 tccgcgaaag agtatcgaaa gctcactttg ttggttggag caacccaacc atcatttaat
    21721 ccctgcggac agcgaatttt atcctcctca acttcaggcg acgacagatt accccggcgc
    21781 actgtttgtt gaaggagaac tgcacgcgct gcattcattt cagcttgcca tagtggggag
    21841 tcgggcgcat tcatggtatg gcgagcgatg gggacgatta ttttgcgaaa ctctggcgac
    21901 gcgtggagtg acaattacaa gtggactggc gcgtggaatc gatggtgtgg cgcataaagc
    21961 ggccttacag gtaaatggcg tcagcattgc tgtattgggg aatggactta ataccattca
    22021 tccccgccgc catgcccgac tggctgccag tttgcttgaa catggcggag ctctcgtctc
    22081 ggaatttccc ctcgatgttc caccccttgc ttacaatttc ccacgaagaa atcgcattat
    22141 cagtggtcta agtaaaggtg tactggtggt ggaagcggct ttgcgcagtg gttcgctagt
    22201 gacagcacgt tgtgcgcttg agcaggggcg tgaagttttt gccttgccag gtccaatagg
    22261 gaatccggga agcgaagggc ctcactggtt aataaaacaa ggtgcgattc ttgtgacgga
    22321 accggaagaa attctggaaa acttgcaatt tggattgcac tggttgccag acgcccctga
    22381 aaattcattt tattcaccag atcagcaaga cgtggcattg ccatttcctg agctcctggc
    22441 taacgtagga gatgaggtaa cacctgttga cgtcgtcgct gaacgtgccg gccaacctgt
    22501 gccagaggta gttactcaac tactcgaact ggagttagca ggatggatcg cagctgtacc
    22561 cggcggctat gtccgattga ggagggcatg ccatgttcga cgtactaatg tatttgtttg
    22621 aaacctatat tcacacagaa gctgagttgc gtgtggatca agacaaactt gaacaggatc
    22681 ttaccgacgc aggatttgat cgagaagata tttacaatgc cctgctttgg ctggaaaagc
    22741 ttgcggatta tcaggaagga ctggcagaac cgatgcaact ggcctctgat ccactctcca
    22801 tgcgtattta tacccctgag gagtgtgaaa gactggatgc cagctgtcgt ggttttctgc
    22861 ttttccttga gcagattcag gtgctcaacc ttgaaactcg tgaaatggtg atagagcgag
    22921 tgctcgcgct ggataacgca gagttcgaac tggatgatct gaaatgggta atcctgatgg
    22981 tgttgttcaa tattccgggc tgcgaaaatg cgtaccagca aatggaagaa ttactctttg
    23041 aagtgaatga aggtatgctg cattaatcat ttctttaatt cagcataagt tgttatggcg
    23101 aaatcagcac tgttcacggt gcgtaataat gagtcctgcc caaagtgcgg ggctgaactg
    23161 gttattcgat ccgggaaaca cggtccgttt cttggatgct cacagtatcc ggcgtgtgac
    23221 tacgtccgtc ctctgaaatc ttcagcggat ggacatattg tcaaagttct ggaggggcag
    23281 gtttgccctg catgtggcga aaatctggta ttacgccagg gacgctttgg tatgtttatt
    23341 ggttgcagta actaccctga atgcgaacat accgaactta tcgataaacc ggacgaaaca
    23401 gcaattacat gcccccaatg ccggacgggc catctggtcc agcgccgctc ccgttatggc
    23461 aaaacatttc actcctgtga tcgctacccg gagtgtcaat ttgccattaa cttcaaaccc
    23521 atagctggag aatgccctga gtgtcattat ccactactca tcgaaaagaa aaccgcgcag
    23581 ggtgtaaaac acttttgtgc cagtaaacaa tgtggaaagc cggtttcggc ggaataataa
    23641 cgtgaataat aacctgcaag gagacgctat cgcagctgcg atagatgtcc tcaatgaaga
    23701 acgtgtcatc gcctatccaa cggaagccgt tttcggtgtc gggtgcgatc ctgatagcga
    23761 aacagcagtg atgcgactgt tggagttaaa acagcgtccg gttgataagg ggctgatttt
    23821 aatcgcggca aattacgagc agcttaaacc ctatattgat gacaccatgc tgactgatgc
    23881 gcagcgtgaa accatttttt cccgctggcc aggtcctgtc acctttgtct ttcccgcgcc
    23941 tgcgacaaca ccgcgctggt tgacgggccg ctttgattcg cttgctgtac gagtcaccga
    24001 ccatccgttg gtggttgctt tgtgccaggc ttatggtaaa ccgctggttt ctaccagtgc
    24061 caacttgagt ggattgccac cttgtcgaac agtagacgaa gttcgcgcac aatttggcgc
    24121 ggcgttcccg gttgtgcctg gtgaaacggg ggggcgttta aatccttcag aaatccgcga
    24181 tgccctgacg ggtgaactgt ttcgacaggg gtaacataat ggaaacctat gctgtttttg
    24241 gtaatccgat agcccacagc aaatcgccat tcattcatca gcaatttgct caacaactga
    24301 atattgaaca tccctatggg cgcgtgttgg cacccatcaa tgatttcatc aacacactga
    24361 acgctttctt tagtgctggt ggtaaaggtg cgaatgtgac ggtacctttt aaagaagagg
    24421 cttttgccag agcggatgag cttactgaac gggcagcgtt ggctggtgct gttaataccc
    24481 tcaagcggtt agaagatgga cgcttgcagg gtgacaatac cgatggtata ggtttgttaa
    24541 gcgatctgga acgtctgtct tttatccgtc cgggtttacg tattctgctt atcggcgctg
    24601 gtggagcatc tcgcggcgta ctactaccac tcctttccct ggactgtgcg gtgacaatca
    24661 ctaatcggac ggtatcccgc gcggaagagt tggctaaatt gtttgcgcac actggcagta
    24721 ttcaggcgtt gggtatggac gaactggaag gtcatgagtt tgatctcatt attaatgcaa
    24781 catccagtgg catcagtggt gatattccgg cgatcccgtc atctctcatt catccaggca
    24841 tttattgcta tgacatgttc tatcagaaag gaaaaactcc ttttctggca tggtgtgagc
    24901 agcgaggctc aaagcgtaat gctgatggtt taggaatgct ggtggcacag gcggctcatg
    24961 cctttcttct ctggcacggt gttctgcctg acgtagaacc agttataaag caattgcagg
    25021 aggaattgtc cgcgtgaatc aggccatcca gtttccggac agagaagagt gggacgagaa
    25081 taaaaaatgt gtatgttttc ccgctctcgt gaatggcatg caactgacat gtgcgatctc
    25141 tggcgagagt ctggcgtatc gttttactgg agatacgcca gaacagtggt tagcgagttt
    25201 tcgtcagcat cgctgggatc tggaagaaga agcggaaaac ttaattcagg aacaaagtga
    25261 agatgatcaa ggctgggtct ggttaccctg atccagatat tcgtccttcc atttcacgta
    25321 attattcgcg gaatagcgta acccagcctt ctcttcatca cttaacgggc ggatctgttt
    25381 gacggggcta ccgagataca gatatccgct ctccagccgt ttattttgtg ggaccagact
    25441 acccgcacca atcatcacat catcttctac tattgcgcca tcaagtaaaa ttgagcccat
    25501 cccaaccaaa actcgattgc caatggtgca gccgtggagc atcaccttgt gaccaacagt
    25561 gacatcttcg ccaatggtta atgggttgcc atctgggttg tacgaggatt tatgagtgac
    25621 atgcaacata ctgccatcct ggatattggt gcgtgctccg atctgtacat aatgtacatc
    25681 tccacgaatc acaacgagcg gccagatccc cacatcatca gccagacgaa cgtcaccaat
    25741 cacgacactg ctatcgtcga tcattacgcg ctgaccgatt tgtggaaaaa gatcgcggta
    25801 tgggcgtaaa acatcagaca tacttacctc agcaataaat gatttactaa tgactttggg
    25861 ggcattattg gccttgtgca agtcttttag tatgcaaaaa agcaccgttt tgtgtgcgat
    25921 tgcagcaaaa agggtgaaaa aacaacaaac agaaaaaaag atcaaaaaaa tacttgtgca
    25981 aaaaattggg atccctataa tgcgcctccg ttgagacgac aacgtgaaac acttcacagg
    26041 atggtcggaa caacgaagag
//
LOCUS       DAZFXT010000062        25732 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.062, whole genome shotgun sequence.
ACCESSION   DAZFXT010000062 DAZFXT010000000
VERSION     DAZFXT010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.062"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            323..742
                     /locus_tag="ACHKW0_002773"
     CDS             323..742
                     /locus_tag="ACHKW0_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7475579.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            742..2010
                     /gene="umuC"
                     /locus_tag="ACHKW0_002774"
     CDS             742..2010
                     /gene="umuC"
                     /locus_tag="ACHKW0_002774"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HFO7475580.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(2056..2586)
                     /gene="dsbB"
                     /locus_tag="ACHKW0_002775"
     CDS             complement(2056..2586)
                     /gene="dsbB"
                     /locus_tag="ACHKW0_002775"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HFO7475581.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(2732..4273)
                     /gene="nhaB"
                     /locus_tag="ACHKW0_002776"
     CDS             complement(2732..4273)
                     /gene="nhaB"
                     /locus_tag="ACHKW0_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HFO7475582.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4495..5214
                     /gene="fadR"
                     /locus_tag="ACHKW0_002777"
     CDS             4495..5214
                     /gene="fadR"
                     /locus_tag="ACHKW0_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HFO7475583.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(5266..6798)
                     /locus_tag="ACHKW0_002778"
     CDS             complement(5266..6798)
                     /locus_tag="ACHKW0_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HFO7475584.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            7128..8426
                     /gene="dadA"
                     /locus_tag="ACHKW0_002779"
     CDS             7128..8426
                     /gene="dadA"
                     /locus_tag="ACHKW0_002779"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HFO7475585.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            8436..9506
                     /gene="dadX"
                     /locus_tag="ACHKW0_002780"
     CDS             8436..9506
                     /gene="dadX"
                     /locus_tag="ACHKW0_002780"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HFO7475586.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(9892..11628)
                     /locus_tag="ACHKW0_002781"
     CDS             complement(9892..11628)
                     /locus_tag="ACHKW0_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006884 - cell volume homeostasis [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HFO7475587.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(11723..12637)
                     /gene="ldcA"
                     /locus_tag="ACHKW0_002782"
     CDS             complement(11723..12637)
                     /gene="ldcA"
                     /locus_tag="ACHKW0_002782"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HFO7475588.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            12737..13348
                     /gene="emtA"
                     /locus_tag="ACHKW0_002783"
     CDS             12737..13348
                     /gene="emtA"
                     /locus_tag="ACHKW0_002783"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HFO7475589.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(13350..14084)
                     /locus_tag="ACHKW0_002784"
     CDS             complement(13350..14084)
                     /locus_tag="ACHKW0_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HFO7475590.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKLL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            14285..14539
                     /locus_tag="ACHKW0_002785"
     CDS             14285..14539
                     /locus_tag="ACHKW0_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HFO7475591.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGIFRLLRRE"
     gene            14717..15157
                     /gene="ycgY"
                     /locus_tag="ACHKW0_002786"
     CDS             14717..15157
                     /gene="ycgY"
                     /locus_tag="ACHKW0_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcgY"
                     /protein_id="HFO7475592.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     SQRRRKSPGDESETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(15236..16933)
                     /gene="treA"
                     /locus_tag="ACHKW0_002787"
     CDS             complement(15236..16933)
                     /gene="treA"
                     /locus_tag="ACHKW0_002787"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7475593.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPHQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYMWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(17253..18671)
                     /gene="dhaM"
                     /locus_tag="ACHKW0_002788"
     CDS             complement(17253..18671)
                     /gene="dhaM"
                     /locus_tag="ACHKW0_002788"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA];
                     GO_process: GO:0019563 - glycerol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase phosphoryl donor subunit
                     DhaM"
                     /protein_id="HFO7475594.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVAPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKRQRFNRQG"
     gene            complement(18682..19314)
                     /gene="dhaL"
                     /locus_tag="ACHKW0_002789"
     CDS             complement(18682..19314)
                     /gene="dhaL"
                     /locus_tag="ACHKW0_002789"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047324 - phosphoenolpyruvate-glycerone
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HFO7475595.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(19325..20395)
                     /gene="dhaK"
                     /locus_tag="ACHKW0_002790"
     CDS             complement(19325..20395)
                     /gene="dhaK"
                     /locus_tag="ACHKW0_002790"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HFO7475596.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECAMQIDGGEGVLMI
                     IKNYTGDILNFETATELLHDSRVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            20623..22542
                     /gene="dhaR"
                     /locus_tag="ACHKW0_002791"
     CDS             20623..22542
                     /gene="dhaR"
                     /locus_tag="ACHKW0_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase operon transcriptional
                     regulator DhaR"
                     /protein_id="HFO7475597.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALLILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(22642..25508)
                     /locus_tag="ACHKW0_002792"
                     /pseudo
     CDS             complement(22642..25508)
                     /locus_tag="ACHKW0_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT         6371 a         6382 c         6609 g         6370 t
ORIGIN      
        1 aactattaat aaattttact tcatctaatt catagttagc cgggcgggat gcgtcaatgt
       61 ctttatttct attaatatga taaatatcaa acaatgttta atgtcattat ggcgaatgct
      121 tctattctat ttttttgccg ggtgatattt ttcatttctg ctggatgagc gtcgtcgcca
      181 gaaggccacg tgagcacagg ataagagaac gaaaaatcag cagtctatgc agcgacaaat
      241 attgatagcc tgaatcagta ttgatctgct ggcaagaaca gactactgta tataaaaaca
      301 gtataacttc aggcagatta ttatgttgtt tatcaagcct gcggatctcc gcgaaattgt
      361 gacttttccg ctatttagcg atcttgttca gtgtggcttt ccttcaccgg cagcagatta
      421 cgttgaacag cgcatcgatc tgaatcaact gttgatccag catcccagcg cgacttactt
      481 cgtcaaagca agtggtgatt ctatgattga tggtggaatt agtgacggtg atttactgat
      541 tgtcgatagc gctattaccg ccagccatgg tgatattgtc atcgctgctg ttgacggcga
      601 gtttacggtg aaaaaattgc aactacgccc gacggtacag cttattccca tgaacagtgc
      661 gtactcgccc attaccatca gtagcgaaga tacgctggat gtctttggtg tggtgatcca
      721 cgtcgttaag gcgatgcgct gatgtttgct ctctgtgatg taaacgcgtt ttatgccagc
      781 tgtgagacgg tgtttcgccc tgatttatgg ggtaaaccgg tggttgtgct atcgaataat
      841 gacggttgcg ttatcgcccg aaacgctgag gcaaaggcgc tgggtgttaa aatgggcgat
      901 ccctggttca aacaaaaaga tctgtttcgt cgctgtggcg tggtttgctt tagcagcaat
      961 tatgagcttt acgcagacat gagcaatcgg gtgatgtcga cgctggaaga actatcgccc
     1021 cgcgtcgaga tttacagtat tgatgaggca ttctgcgatc tgacaggtgt gcgtaattgt
     1081 cgcgatctga ctgattttgg cagagaaatt cgcgcaacgg tgctacaacg tacccatctt
     1141 actgttggtg tggggatcgc ccagaccaaa acgttggcta agcttgccaa tcatgcggca
     1201 aaaaaatggc agcggcagac gggtggggtg gtggatttat caaatctgga acgccagcgt
     1261 aaattaatgt ctgctctccc cgtggatgac gtctggggga ttggacggcg gatcagcaaa
     1321 aaactggacg cgatggggat caaaaccgtt ctcgatttgg cggatacaga tatccggttt
     1381 atccgtaaac attttaatgt cgtgctcgaa agaacggtgc gtgaactgcg cggcgaaccc
     1441 tgtttgcaac tggaagagtt tgcaccgacg aagcaggaaa ttatctgttc ccgctcgttt
     1501 ggtgaacgca tcacggatta tccatcgatg cggcaggcca tttgtagtta cgctgcccgg
     1561 gcggcggaaa aacttcgcag cgagcatcaa tattgtcggt ttatctccac atttattaag
     1621 acgtcaccat ttgcgctcaa tgaaccttat tacggcaata gcgcgtcggt aaaactgctg
     1681 acgcccactc aggacagcag ggatatcatt aacgctgcta cgcgatctct ggatgccatc
     1741 tggcaagcgg gccatcgcta ccaaaaagcg ggcgtgatgc tgggggattt cttcagtcag
     1801 ggagtcgcgc agctcaattt attcgatgac aacgcaccgc gccccgggag tgagcaattg
     1861 atgacggtaa tggatacact gaatgctaaa gagggcagag gaacactcta ttttgccggg
     1921 caggggatcc agcaacaatg gcagatgaag cgtgccatgc tttcaccacg ttatacaacg
     1981 cgaagttctg atttactgcg ggtcaaataa atatagcggc aggaaaaaag cgctcccgca
     2041 ggagcgctga agggattagc gaccgaacag gtcacgtttt ttcgctttaa acggctggga
     2101 aatcaccacc agcaccgcga caatcaggta agcgataaaa ataccgagca gccactgcgg
     2161 catttccaga cctaaaaaat cccactgacg ctcggcacaa tcgccagagg cgacaaacac
     2221 ttgcggcacc catttatcca gcggcagcca ttccgggaaa cgaaccataa aatcacaggt
     2281 ggcaaacggc gaaggataga gctgaagcat ggtgtgctcg taagttaact gcacaccgcg
     2341 gaacgcacta tacaaccaga taaccatcgc tacataacgc agcggagttt tcggggcgat
     2401 cgcgccaatc agcgcagcac ccagaacgcc gaataacgca caacgttcat aaatacagag
     2461 cacgcaaggt ttcagtaaca tcacatgctg gaaccacagc gccgtcagtt ccagtgccag
     2521 agcagtaaac gccatcaaca gccacgcgcc ccggccttgt gaacattggt tcaaaaatcg
     2581 caacataatc atttccctgc aatatgcata gagtgcgcag tttaaaccaa ttcattcgct
     2641 gcgccaccag gaggcagcaa aaataggaag caattggaca attatcgggc gaaaaggcaa
     2701 accgggcatt tatatgcccg gtaagttgtt atcaaagcgt tgctatccag cccatttgca
     2761 taaaccattc ggttacaggg gcaagcgtaa actcgacgca gagcaagccg acgagtgtca
     2821 gtacgagggt gtaaggcagg gccatccaca ccatgcggcc ataagagagg cgaatcaatg
     2881 gcgcgagtgc agaggtcagc aggaacagga acgcagcctg accgttcggc gtagcgacgg
     2941 agggcagatt ggtaccggta ttaatggcga ccgccagcag ctcgtattgc ttcaacgtga
     3001 tagcgccact ttccattgcc gctttcgctt cgttgatata aatcgtcccc acgaagacgt
     3061 tatccgaaat ggatgacagc agaccgttga aaatatagaa cagcgacagc tgagcatgtt
     3121 ccgatgcctg caacacaaac tgaataattg gcgaaaacag ttgttggtcg ataatcaccg
     3181 cgactaccga gaaaaacacc gtcaacagtg cggtgaatgg cagagattcg gtgaaggctt
     3241 taccgatagc atgttcatcg gtgacaccgg tcaatgatgt cgccagaata atgactgaca
     3301 aaccaatcaa gccaacttcc gccagatgca acgccagcgc agtcaccagc cagacgccaa
     3361 taatcgcctg gacaatcaga cggattttat cctgacgggt acgctggtgg cggctttgat
     3421 cgtcaaactg ttgcaacact tcgcggactt tctccggcag cgtttcaccg tagccaaacc
     3481 aacgcagctt ctctaccagc aggcaggtta acaggccaca aatcagaacc ggaacggtca
     3541 ccggcgacat gcgcaggaag aaatcgccaa aatgccagcc agccgcttta gcgatgatca
     3601 ggttctgtgg ttcgcccacc atggtcatca cgccgcctaa tgcagtgccg acaccggcat
     3661 gcatcatcag gctacgcaga aagccacgga actgttccag aaccactttg taatgcttgt
     3721 cgatatgact atcgtcttgc aggtcggtat cttcggtacg ggaagaggct acgcggtgat
     3781 aaataccata aaaaccgaca gcaacgctga tcaccaccgc cacgacggtt aaggcatcga
     3841 ggaacgcgga gaggaacgct gccgccacgc aaaaagagag cgacagcagc attttggagc
     3901 gaatgcttaa cagcaaacgg gtaaatatga acagcaacag ctgtttcata aaatagatac
     3961 ccgccaccat aaacatcaat aacagcaaga cttcaagatt tgccgccacc tcttcacgga
     4021 cgtgttccgc gctggtcatg ccgatgaata ccgcttcgat agccaacaga ccaccgggga
     4081 gcagcgggta gcatttcagg gccatcgcca gagtgaaaat aaattccgct accagcaacc
     4141 agcccgcgac gaaagggctg atgaggaaaa ttaacgggtt tacgattaag aaaatgatga
     4201 gggcgagttt gtaccagtcg ggggactggc ccaaaaaatt gcgccatagc gcgcggcccc
     4261 aggagatctc catgatggtt tcccttacct tacaaataat caatgatgtt tttatgttta
     4321 aacgcaaagc ttaacggtca ggcaggagtg aggcaagtct tgatagtcaa ggggaaagag
     4381 atgcggaaaa tgaagccttg atcccttttt tcttcttttt gtctgctatc agcgtagtta
     4441 gccctctggt atgatgagtc caactttgtt ttgctgtgtt atggaaatct cactatggtc
     4501 attaaggcgc aaagcccggc gggtttcgcg gaagagtaca ttattgaaag tatctggaat
     4561 aaccgcttcc ctcccgggac tattttgccc gcagaacgtg aactttcaga attaattggc
     4621 gtaacgcgta ctacgttacg tgaagtgtta cagcgtctgg cacgagatgg ctggttgacc
     4681 attcaacatg gcaagccgac gaaggtgaat aatttctggg aaacctctgg tttaaatatc
     4741 cttgaaacac tggcgcgact ggatcacgaa agtgtgccgc agcttattga taatttgctg
     4801 tcggtgcgta ccaatatttc cactattttt attcgcaccg cgtttcgtca gcatcccgat
     4861 aaagcgcagg aagtgctggc taccgctaat gaagtggccg atcacgccga tgcctttgcc
     4921 gagctggatt acaacatatt ccgcggcctg gcgtttgctt ccggcaaccc gatttacggt
     4981 ctgatcctta acgggatgaa agggctgtat acgcgcattg gtcgtcacta tttcgccaat
     5041 ccggaagcgc gcagtctggc gctgggcttc taccacaaac tgtcggcgtt gtgcagtgaa
     5101 ggcgcgcacg atcaggtgta cgaaacagtg cgtcgctatg ggcatgagag tggcgagatt
     5161 tggcaccgga tgcagaaaaa tctgccgggt gatttagcca ttcaggggcg ataatccctt
     5221 ccgtttaaag agcaaacccc tcaaacgagg ggttttttgt tgtttttaca gatttcccat
     5281 tcttggcggg caacgttcca gcaactcgat gctgccgtct tcgttttgct gttcgagcat
     5341 cacatcaaat ccccacaggc gatgcacatg cttcagcact tctttgcgcc cccgatccag
     5401 cggtgcgcga ttatgtggaa tgtaacgcag cgtcagcgaa cggtcgccgc gcaaatccac
     5461 gttccagatc tgaatattcg gctccagatt acttaagtta tattgcgacg ataaccggtt
     5521 acggatctcc cgataacctt cttcattatg aatagcggaa atctccagat aattatgccg
     5581 atcatcatcc agcacggtga agaagcggaa atcacgcatc actttcggtg acagaaactg
     5641 gctgataaaa ctctcatctt tgaaatcacg catcgcgaaa tgcagcgttt ccagccagtc
     5701 ggaaccggcg atatccggga accagtattt gtcttcttcc gttggcgact gacaaatccg
     5761 tttaatatcc tggaacatgg cgaacccgag ggcatatggg ttgatgccgc tgtaccacgg
     5821 gctgttatag gggggctgga agaccacatt ggtgtggctg tgcaaaaact ccagcataaa
     5881 acgttccgtt actttccctt catcatacag atggttaagg atggtatagt gccagaaggt
     5941 cgcccagcct tcgttcatca cctgagtctg tttttgcgga taaaaatact ggctcacctt
     6001 acgcacaata cgcaggattt cacgctgcca tgattccagc agtggcgcat ttttctccat
     6061 aaaatagagc aggttttctt gtggttcgga cggatagcgg cgcgcttcag caaccgtttt
     6121 ctcttcctcg cgcttcggca gggtacgcca gagcatattg acctgacttt gtagatactc
     6181 ttcgcgactt ttctgccggg ctttctcttc ttgcagcgag attttttgcg ggcgtttgta
     6241 ccggtccacg ccgtagttca tcagcgcgtg gcacgagtcc agaagccgtt ctacttcatc
     6301 aacgccataa cgttcttcgc actcggtaat atatttacgg gcaaaaatca gataatcgac
     6361 aatcgaactg gcgtcggtcc aactgcggaa taagtaattg tttttaaaga aagagttatg
     6421 cccatagcag gcgtgtgcca tcaccagcgc ctgcatggta atggtgttct cttccatcag
     6481 gtaagcgata cacgggttag agttaatgac gatttcatag gccagtcctt gctgaccgtg
     6541 cttatacagc cgttcagtct cgataaactt tttaccgaat gaccagtgcg gatagttaat
     6601 tggcatgccg acgctggagt aggcatccat catctgttct gaggttatca cttcaatctg
     6661 gtgcgggtag gtatccagcc ggtagagttt cgccacccgg tctatctctg ccagataaac
     6721 atccagcagg tcgaacgtcc agtcgggtcc atcgctcaaa cgtgtggtgt ccttattcat
     6781 agaatcgatc gtcgccatac gcgcacctca ttgttgtcgg cgctctctgt gtggagcacc
     6841 tcatttcaag catagaacac ctgttaaaaa ccgcgtcgcc ggagaatttt tttctttgcg
     6901 atttcttatt atcagagtgc cactaatccg cttctgaacg gaattttatg ctggataaaa
     6961 agggcgttca gcaggagata ctaaagacgc catattgccg cagagtcagg gagatgtgag
     7021 ccagctcacc ataaaaaagc cgtatgttga ataatatttt caactgagtt atcaagatgt
     7081 aattagatta ttattctttt actgtatcta ccgttatcgg agtggctatg cgagttgtca
     7141 tactgggaag tggtgtggta ggcgttgcca gcgcctggta cttaaatcag gcaggacatg
     7201 aggtcaccgt cattgatcgg gagccggggg ccgccctgga aaccagcgcc gcgaatgccg
     7261 ggcaaatctc ccccggatat gctgcaccgt gggcggcacc aggtgtgcct ttaaaagcga
     7321 tcaaatggat gttccagcgc catgcgccgc tggcggtccg tctcgacggt acgcagtttc
     7381 agttgaaatg gatgtggcaa atgttacgta actgcgacac cagccactac atggaaaaca
     7441 aagggcggat ggtgcgtctg gcggaataca gccgtgattg cctgaaagca ttgcgcgccg
     7501 aaaccaatat tcagtatgaa gggcgtcagg gtgggacgct gcaactgttc cgtaccgaac
     7561 aacagtatga aaatgcgacc cgcgatatcg ccgtgctgga agatgccggc gtaccgtatc
     7621 agctgctgga atccagccgc ctggcggaag tggagcccgc gctggcagaa gtagcgcaca
     7681 aactgacggg tggtctgcaa ctgcccaatg atgaaaccgg agactgccag ctgtttaccc
     7741 agaatctggc gcggatggca gagcaggcgg gggttaaatt ccgctttaat acgcccgttg
     7801 accaactgct ttgcgacggc gagcaaatct acggcgtgaa gtgtggcgat gaagtgatta
     7861 aggccgatgc gtatgtgatg gcgtttggtt cttactcgac ggcgatgctc aaaggcattg
     7921 ttgatattcc ggtgtatccg ctgaaaggct actcgctgac tattccaatt gcgcaggaag
     7981 atggtgcgcc agtatccact attcttgatg aaacctacaa aatcgccatt acccgtttcg
     8041 ataaccgcat tcgtgttggc ggaatggcgg aaattgttgg ttttaacacc gagctgttgc
     8101 aaccgcgtcg tgaaacgctg gaaatggtgg ttcgcgatct ctatccacgc ggcggtcatg
     8161 tcgagcaggc gactttctgg acgggtttgc gcccgatgac gccagacggt acgccggttg
     8221 tcgggcgtac acgctttaaa aatctgtggc tgaataccgg tcacggcacg ctcggctgga
     8281 cgatggcttg cggttccggt cagttgttaa gcgatctgct ctctggtcgc acgccagcga
     8341 tcccatatga ggatctaagc gtagcgcgct acagccgtgg atttacgcca tcacgtccgg
     8401 gccatttaca tggcgcacac agctaaggaa acgagatgac ccgtccgata caggccagcc
     8461 tcgatctgca ggcattaaaa cagaatctgt ccattgtccg ccaggccgcg ccgcacgcgc
     8521 gcgtctggtc ggtggtaaaa gcgaacgctt acgggcatgg tattgagcgt atctggagcg
     8581 cgctcggggc caccgatggc tttgcattac ttaacctgga agaggcaata acgttacgtg
     8641 agcgcggctg gaaggggccg atcctgatgc tggaaggatt tttccatgct caggatctgg
     8701 agatttatga ccagcaccgc ctgaccacct gcgtacacag caactggcag ctcaaagcac
     8761 tgcaaaatgc gcggctaaaa gcaccgttgg atatttatct taaagtgaac agtgggatga
     8821 atcggttggg cttccagccc gatcgcgtgc ttaccgtctg gcagcagttg cgggcaatgg
     8881 cgaatgttgg cgagatgacc ctgatgtcgc attttgccga agcggaacat cctgatggaa
     8941 tttccagcgc gatggcgcgt attgagcagg cggcggaagg gctggagtgt cggcgttcgt
     9001 tgtccaattc ggcggcgact ctgtggcacc cggaagcgca ttttgactgg gttcggcctg
     9061 gcattatttt gtatggcgct tcgccgtccg gtcagtggcg tgatatcgcc aataccggat
     9121 tacgtccggt gatgacgcta agcagtgaga ttattggtgt ccagacgcta aaagcgggtg
     9181 agcgtgtggg ctacggcggt cgctatactg cgcgcgatga acagcgaatc ggcattgtcg
     9241 ccgcagggta cgccgacggt tatccgcgcc acgcgcctac cggtacccct gttttagtgg
     9301 acggcgtgcg caccatgacg gtggggaccg tctcgatgga tatgctggcg gtcgatttaa
     9361 cgccttgccc gcaggcgggg attggtacgc cggttgagct gtggggcaag gagatcaaaa
     9421 ttgatgatgt cgccgccgct gccggaacgg tgggctatga gttgatgtgc gcgctggcgt
     9481 tacgcgtccc ggttgtgaca gtgtaacttg ttgtaagccg gatcggagac agcgtcttct
     9541 ggttgcgaaa aaatcatcca tccggctggt cagcaactgt agttgttaat gtgacagagc
     9601 cattgcccat gatagtgtcc attaaaagaa tggacactat ttccccggaa cctgaactca
     9661 ccgcacaggc gttctacata aaacgcttac gcttcattgt tgactcgaac tcgacttcag
     9721 ataaatcacg ctttaccctt gatggagcct gtacatagat ttgtgtaatt gcctgatttt
     9781 gatatgttca attcaacatc aaatgaaggt taaattatgg acgacaaaca attgcaggct
     9841 caggctgcgt tcagcaaagc atcgcaaccg gcgatagatg cttcattaaa tttaagattc
     9901 agcttcctct tcagccaccc gtacgccaat cttcaacact tcattatctt ctttctcggc
     9961 caccgtccag atcatcccgg caaactctac ctggtcaccg acaaccggtg ccgcgcctaa
    10021 caactgctgg acaatttcac ccagcgtttg ctgcttatca cgatattctc gcccgtcttc
    10081 caggccatat atcagcgcaa catcagcata tttggcgctg gcttcgagaa tgaagtcacc
    10141 aaagaagcgt tgatccagcg cgaccggcgg cgactggctg aacagtttac cgagcgccgg
    10201 aagatcgcgt tcccgaccaa ttacacacaa cacatcgcct tcgcgcagtc gggtgctgcc
    10261 ggtaggatga agcaactggt tatcacgaaa cagtgccgca atacgcgtct cttttggcat
    10321 atgcaaatcg cgcagtgccg cgcccacgca ccatttatcg gcactcaatt gataaacaaa
    10381 ctgctcccac ggattttccg gatgaatatc caggccaacg cgtgacaccg gacgtcccac
    10441 tggcggaacc accactttgg cttttttagc cgcccacgag agtgatgttc cctgcaagag
    10501 cagtgaaacc agaaccacaa agaaggcgac attaaagaac agacgtgcat tctccagccc
    10561 cgccatcatc gggaacacgg ccaggatgat tggcaccgcg ccgcgtaatc ctacccagct
    10621 gataaacacg cgctcacgca gattgaaacc gcggaagggg agcaatccag caaataccga
    10681 aaggggacgg gcgaagaata tcatccatgc cgacaaaatg agcgccggaa tggcaatagg
    10741 cagcagatcg cttgggttaa ccaatagccc cagcaccagg aacatggcga tttgcgccag
    10801 ccaggcgagg ccgtcgaaat tttgcaggat gccgtagcgg ttgcgaatcg gccgattacc
    10861 cagcagaaaa ccgcacagat aaacagccag aatgccgctg ccttccagcg ccgtggttaa
    10921 agcaaaaatt aaaataccgc cgcttaatgc cagcaatgga tataatccgg caggcagggc
    10981 gatgcgatta atcatttgca gcagtaaata accaccgcca agtccgatga caatcccgag
    11041 gccaaattgc tgcagaatat cgaccacgaa catccaactg acactgcttt catgttgctg
    11101 gatcatcgca atcagggtaa tcgtcagaaa gaccgccatt ggatcattac tgccggattc
    11161 aatttccagc gtcgagccaa cacgttcgtt aagcccctta ccacccagca gagaaaagac
    11221 cgctgcagca tcggtagagc cgacgatagc gccgattaat aagccttcaa tcaaatcaag
    11281 attaaacagc cacgccgcca tcatgccggt taaaccagag gtgataagca cgcccagcgt
    11341 cgccagcgac agtgccggtc ctaacgccac acgaaaggaa ctggcctgag tgcgcatccc
    11401 gccgtcgagc aaaataatcg ccagtgccag gttacttacc atgtaggcga aggggtaatt
    11461 atcaaacggg ataccgccga cgccatcgac tcctgccagc atgccgatcg ccagaaaaat
    11521 aaccagaata ggaatgccaa gacgggaaga aaatgaacta agtaatatac tgctggtgac
    11581 aaggatggat cctaagatga aaaggctaat tattgttgtg gcatccaacg gtcggttact
    11641 cctgattacg ctgtctctta tataaaccct accatattag cggcagagac agcgttttac
    11701 ttagccctga agcgtgattt ttttacattt taagaacagg atgaccgcta atcgtcaact
    11761 gagtgccttc ccgggtgtta ttcagaatgg catgtgcgcc caacggcagc gtaaccgtgc
    11821 gttgttcatg accaaaatcg aggccggtaa tgagtggaat cgacaggcgg gaacgcaaaa
    11881 acgcgtacac tgactccagg ttgtaacctg cgtcataatc attgggcgtg ctgccgctaa
    11941 agctaccgag aataatcgcc ttctgacgtg gcaaaattcc cgcatgatag agctgcaaca
    12001 gcatacgttc gacgcggaaa gggtgctcgt taatatcttc cagcaccaga ataccgttct
    12061 caatttttgg catccacggt gtaccaatca gtgaaatcag catcgcaaga ttgcctcccc
    12121 acaacgtgcc ttcggtctga catgtcgggc cttccccttg ccattcaatg gtgaaggttt
    12181 tattgcgtaa cgccagccag aaatggtgct cggtaaaggc gttcagttca tccgcaccaa
    12241 agtttgctac cagcatgggg ccgctaaagg tgatgacatt gccttgcgcc agaagaccgc
    12301 actgaatggc ggtaaaatcg ctatgtccgc aaatgagtaa cgggtcatgt tgttggcgag
    12361 ccaccagtgc ctgccagtca atatctgcca gtaaacgact ggcaccgtaa ccgccgcgta
    12421 cagccagcac gatggtgttg ggggtagtca gtctggcaag ggaattgaga tcttccaggc
    12481 gctccgtttc cgtacccgca aaacgctcac aacgacgggc aataacctcg acgttattga
    12541 cctgatgccc ctcgtcggta aggcgatgga taccacgcag cgcggcgtgc tgtttaatgc
    12601 agtaacccga tggggcaatt aagtgaaaca gagacatggc aattccttgc tgacaacaga
    12661 aacgaaatgt atatcatgcc gcttaggtgt gccgttgtca cctcaacggc gattccaggc
    12721 tataaggata gaagaagtga aattgagatg gtttgccttt ttgattgtgt tattagcggg
    12781 ttgttcatca aagcatgact atacgaaccc gccgtggaac gcgaaagttc cggtgcaacg
    12841 tgcgatgcag tggatgccaa taagccagaa agccggtgca gcctggggcg tcgatccaca
    12901 attgatcacg gcgattatcg ctattgaatc gggtggtaat ccgaacgcgg tgagtaaatc
    12961 gaatgccatt ggtttgatgc agttaaaagc ttcaacctcc ggacgtgatg tttatcgccg
    13021 tatgggctgg agtggtgagc cgacgaccag cgagctgaaa aatccagagc gtaatatttc
    13081 aatgggggcg gcttacctga atattctgga aaccggcccg ctggcaggca ttgaagatcc
    13141 gaaggtactg caatatgcgc tggtggtgtc atacgctaac ggggcaggtg cgctgctacg
    13201 gactttctcg tcagatcgga aaaaggcgat cagcaaaatc aacgatttgg atgctgacga
    13261 gttcctcgat cacgtagcgc gaaatcatcc tgcgccgcag gctccgcgct atatctacaa
    13321 acttgagcag gcactggacg cgatgtaaat cagtcgcgca ctttgtccgc tttttctcgg
    13381 gcttctcgct cgagagagaa aataatccgc tgtaattgcc gctccaccgt cgggctgacg
    13441 ttaagaaaac ggaagctcag acggggagtg gtgatggttt cattcttgcc atcaatcact
    13501 ttgcgctcgc tgatggagat taactgggcg tcaaagtgaa aaacacccca ttgccccatg
    13561 ttgacttcaa tctgagcgaa gcgcatgcct tcttgtaatt cggcaggctt tgctgtttcc
    13621 agtaatgcgc ccatgccgcc taacgacaaa tcatacaggc ggaaacgtaa cgtactgtta
    13681 tccgccagtt tggtctggca aaaataaggc ggatggagtg gggcggagat gcggaaatat
    13741 cggcgtcgtt gtacaaacca taaggtggga ggcggtacgg tgataaatgc cggaagctgc
    13801 aagtattcac tctgctgtag ttgttcaaca gtaaactcga ctttcgcacc ctgagtttcg
    13861 gcggtaatgg taatgtgctg tgcctttagc acggcgatgt tgtcttcggc ttgactgccg
    13921 aaatccagca ccagtttatc cggggttatt gccagtaatt tgctgatcag ctgcccgcca
    13981 ttccaactga gacgcaaagg aattgcggct ttgtgcaaat cgcgtaacac gcccaggacg
    14041 gctaacggat tttgtttcag gaactgctca tggtaatgac tcacgcggaa aaactcctga
    14101 ctgacaaact gtctttggtt tatcggtcac agttaacaaa acttaataca aatgcgtgaa
    14161 tattttttac atgttgttct taaatcagcc gacatacgcc cagcatcgat cccgcccttg
    14221 cctatactta gagcgttgat gtaagcattt cttgcgtccg attcatcgaa acgagggcct
    14281 gaacatggga attattgcct ggattatttt tggcctgata gccggcatta tcgccaagct
    14341 aatcatgccg gggcgtgatg gtggtggatt tttcctgacc tgtattctcg ggatagtcgg
    14401 tgcggtggtc ggcggctggc tggcgaccat gtttggcatt ggcggctcca tcagtggttt
    14461 taatctgcac agcttcctgg tggcggtggt gggagctatt ctcgttctgg gcatattccg
    14521 cctcctgcga agagaataag attttcataa ggcggatagc gatacagatg ccgctatccg
    14581 ctttcacatc agaacgtata ctcgacacct gctttacggg tgaaaaaaat caatatagca
    14641 cttcgatatc cgataaagtc ggttcccggt aacaacaaca gaatcctttt ttaattattg
    14701 tttcgttgtt tttggtgtga ttcagaaaat aaatatttta gacagtagcc agtcaggcgt
    14761 taaaaatggg gcggatagtg aggcggtttt ggctgcgctt attgaacatt tagaattgat
    14821 caatccttct ggcagattaa gccaaaatac ccgttcggca atgcttcagt tacgcgaaga
    14881 ggaatggtct gaattctttt tttggttgct aaattctctg gagtgtctgg attatgtgat
    14941 tattaattta actccagaaa gcaaaaaaac gttgatgagt gaacaccgta acaatattca
    15001 ggtagcaatt gatgctttat atagccaacg tcggcgtaaa tcgccgggtg atgaaagtga
    15061 aacacttacc cgtagaaatg acgctatctt cggcaaccac gtgtggcaaa catttgcgca
    15121 atacttcccg ccaggacttg aaaaaccgtc tgtgtagaaa aaaccatagc tatggtgtta
    15181 tctgccgaca acgccatccg gagaagccga atgacgttgt cggagtaagc gctggttaag
    15241 gtgtgggttg tgcctctttg gttgagggtt gcgtcgttgc tgacttaacg gtcggacgcg
    15301 tcgccggaac attgtcacac ggttgctctt tcgggcagat caaatccagc attttcagcg
    15361 tcacgccatt ggtccagcca aagccatcct gtaatggata ttcgccaccg ccgccccccg
    15421 ttccggtggt gctgacatca tatttttcca ccagcttttt ctcccggtca taggtgtgct
    15481 gaacattggt caggaagtgc cagctaatgt ccatcgccac ctctttttgc ccgtagtttt
    15541 gtaatccttc tgtcgcgacc cactgtaacg gtgcccagcc atttggcgca tcccattgtt
    15601 gcccactttt caccgacgtg gtgttcaggc cgccgggttg cagcagatgt gttttcgtcg
    15661 ccgtcgccat tttgttggcg cgatctttcg ctgccgcatt gacgtacagc gggaacaggg
    15721 cggccgcggt taactgattg cgcactttat gacttttcag gtcgtaatcg gcataccagc
    15781 cttgttgatc gttccacatg tatttttcga tccctttttg acgggcattt gccagcgttt
    15841 cgtactggtt tgccatcgcg ttatctccgg cagctttgct ggcgcgggcg aggatttttt
    15901 ccattttaaa catcaggctg ttcagatcga ccggtacgat gctggtggtg cgtaaggtat
    15961 ttagttgatg cgggttgtcc atccagcgcg agctgaaatc ccagccagac gcagcggcag
    16021 agcgcaggtc gcggtaaatt tcagtggcag gtcgattcgg attgcttttg gcggtggcaa
    16081 tatcttccac ccatgactct ggtcgtggcg tatcgcgatc gtcccagtag cggttgagaa
    16141 gggtaccatc ctgaagtttt acaacgcgtt tttcctgttg tccggcttgc aggttttcaa
    16201 caccgtccat ccagtaagca tattcttttt gcatttgcgg caggtattgc ttcaacgcgg
    16261 catcgccttc atgctgcgcc agtaactcta ccatcagggc aaagaagggc ggttgcgagc
    16321 ggcttaaata gtaactgcgg ttgccgttgg gaatatgacc gtaagtgtct atttcatgag
    16381 caaaattggc caccatatcc gcgactttat cccagtgacc gctttcggca agtcctaaca
    16441 tggtgaagta actgtcccag taatatacct cgcgaaaacg tccgcccggc acgacataag
    16501 gttccggcag cggtaacaga gaatcccatt tttcggtgtt ttcggtagaa cgcgttaata
    16561 ccggccaaag tccgtcaata tgttcgcgca gtgactgccc ctctggcgga acatatttct
    16621 cgccttcttt cggcagggtg aaattgacgt taacgaaatg gcgcagatca aatccgctct
    16681 ggttttgctg catccgataa tcagcaagga tcatcagcgg atcgctgttc ggcacggcat
    16741 cggcaaaggt tttttggtcc ggaaaaagtt tggcgttttg cacatcatta aacagcggcc
    16801 ctaataaaat atcaggcggc tgtggtgtta ccggtgtttc ttctgcctgc accgatagcg
    16861 cagcgaaaca caaaaagata caggctggaa ttaacgccat tttttgcggg cgagaaggtg
    16921 cgggggattt catcaatcat tctcctttgg cgaaaccgaa taaaacggta ttcaagtatc
    16981 agaaaacctt agttcaggat cgcactagct gcatgatcga aatctcattc tgcgaactgt
    17041 caggcatttt agaaaaggga attaaaggga taaataacgg cgaaatagag tgatcaaata
    17101 acgtctgttt attagccagc cagcggcgtt gggttcgtga ttagcgaaga aaaatcatca
    17161 taaattggtg aaaaatataa caggtagtta aaaaccatta gtgctgagta aattgccgga
    17221 tgacatcaga acgatgccat ccgaacagtg gcttaaccct gacggttgaa acgttgcctt
    17281 ttaacgtcca gcgttagcgt ttcttctggt tgtatcgcat acagtttctc accctgctgg
    17341 caaatccagc caatccccag ttcacgggcg attagggcgc tgtgggatac cggacttccg
    17401 gcgctaaggc agataccttt tacaaccgcc ggatccagtt gcagtactgt ggaaggataa
    17461 atgttctccg ccagtagaat agttggcgag ttaaactgcg ggagttcttc tttcgtttgg
    17521 gtcaggtgga ccagggtgcg atgcagaaga tcgtccacat caatatagcg agcttgtaga
    17581 tattcatcat ccagttgctg gtattgctgg ctaagttctt taagaacttg ctgccaggca
    17641 tattctgccg tgcaatgttc atgctgaagg agttcgcttg ccgccgccag cagttccgga
    17701 tcatctaaca gtgtatggtg accagaaaag attgcggcaa tatcgtcaag cccgctggct
    17761 tctgcttttg ctgttaacgt catcagatct aataacgtga agtcaatagc ctggcgtaat
    17821 cgatcttgtt cttcttccac ggtcagggtt gattttgcct gtaccgtaca taaaactggt
    17881 tgataataaa aggctttacc cgaaacaggc ggaacgggac gcagagtagg tggagcgact
    17941 tcctccgttt caccaaagtt atcttcagcc agctgacgga aagcgatcag tgcctcttca
    18001 gcttctggcc ctttcgcaat caggcgcagc gtatcgttat agcgaacttg tagtaacgca
    18061 atctggttaa tactctctgg tgtgacgcat ttgccgtttt tttccagcaa catatcggca
    18121 ttaaatgtcg ataaggtata aaccagccgg gaggccggac gtacatgcag gccgttacgg
    18181 ttttttatga ccaccgccag agaacgggct tcttcatcgt acgcaggaca tgtgtcagag
    18241 attttagtgt cggaggacgg taaacccagt tgttcacgtt tggcttccag cgcatgcata
    18301 gcgtcaaaga taactttgtc gatatccgct cccgaggccg cgctgaccgt tgctgccagt
    18361 gtaccttcga ccaacggcgc agcacacaaa cgtacttttg cggcgatctt gggagccagc
    18421 aattccagcg cagtttcagc actcaataat gcgctaccca tatccatcat gaccagcaca
    18481 tggtcggcat cagcaacaga ttcgatggcc tccatcactt tgacggcatc ggtaccaata
    18541 ggattttgtg gatcgtcaat tcccgcggca atggcgattt tacaactatc actcattaac
    18601 atctgacggg ctaattcacc gacaccttct cccagtcggc tgctatgtga aactatgacc
    18661 aggtttacca tcaccaattc cttactcttt tgcggctaac gccaacattt gcatcataaa
    18721 catcaccgag gtcgcgccgg gatcctggtg accaatactg cgttcaccga gataactggc
    18781 gcggcctttg cgggcttgca tcgtaatcgt actttgtgca gcggattcgg cgattctact
    18841 ggcagcttcg agcgccaccg gaacagagag attttgctcg ctggactgac gtaacgattc
    18901 caccaccggc acccacacat cacacatggt tttatcgcca ggttcggctt tcccgcgact
    18961 gattacgccg tccacgccat cgcggaacat ctgataaagc tcttccagtg tcaggctttg
    19021 ccgtgcctgg gtcgcctgtg cggcgcggat aaagaaggta ccgaacagcg gaccactggc
    19081 accaccgacg ctggaaagca gcgtcatacc ggtattcttg agaatgaaac cgatatcttt
    19141 atctgcgata gcagggagtt tttccaccac tttgctaaag cctcggttca tatttagccc
    19201 gtggtcagcg tcgccaattt cgcgatccag tccggtaaga tactcgctct cggtgctgaa
    19261 aatatcgcca caacgagtga gccagttaac aatttgagtt ctgctcagtg acattgcttt
    19321 ctccttattt accccagtta agggccgggg tgtggaccgg ggcgtcccag agtgccagcg
    19381 tttcgtcatc aactttcagt aaggtgattg agaaaccggt catatccagt gaggtgcagt
    19441 acgcgccaat taaattacgt tcgatagtca atcccgcttg ctggcaacgt gtggtcaggc
    19501 ggttatagac gccgtacagc tcagaaagcg gagttgcgcc aagattgtta accagcgcaa
    19561 tcacccgatc gccagactgg agcggttgtt tggtttgttg ttcttcctgc caactgcctt
    19621 gttgataatc ccagaaacgc aaagtgcgat ggtatgagcc attttccagc agggtgtcga
    19681 acatttcatc gacggtttga tcaagggaag agaaggagcg gcggtcaata cccggctcac
    19741 catgaatgcc gacgccaaac tccatctcat tatccgccag ggtaaaagaa ggtttgcccg
    19801 cggcaggaac ggtacaggca ccgagagcga tacctattga gtggccttga ttattcaact
    19861 tacgccccag ttccgcacag gcgtccagtg agtcgccacg ctccgccgct gcgcctacga
    19921 gtttttcaat taatacggtg ttggcaacgc cgcgtcgccc ggcagtataa agactgtctt
    19981 ttaccgcaac gtcgtcatca atgaccacag tggtcacttt tacgcggcta tcgtgcagta
    20041 actcggtcgc tgtttcaaag ttaagaatat cgccggtgta atttttgata atcatcagta
    20101 caccttcgcc gccatcaatt tgcatggcgc attcaaagat tttatcgggc gtcggtgagg
    20161 tgaaaatttc gcccggacag gcccccgaaa gcatcccctg accgatataa ccacagtgca
    20221 tcggctcgtg tccgctgccg ccacccgaca gcagggcgac ttttcctgca acgggggcat
    20281 cagctcgggt gacatacacc ggatcctgat gcagtgtcag agatggatgc gctttcgcca
    20341 gtcctgccag ttgttcgtcc agtacgtctt gcacatcatt gatcaatttt ttcattattt
    20401 tgctccagca attacggtag ggcatggatg atgttcaacg acacggcgac cactgactgc
    20461 cgatgaatcc attgtgcatc aggcaaggga aaagaaaatt agcccaaatt atgtttcata
    20521 gtgaaacata tgctttaatg aatgttccat attgaaactt ttacgtgtat taatacttaa
    20581 aattgcgagc cggaacacct tttgtcataa gggatgcggg atatgagtgg cgcttttaac
    20641 aacgatggtc ggggcatatc tcccttaatt gcaacctcct gggagcgatg caataagctg
    20701 atgaaacggg agacatggaa cgtaccacat caggcccagg gcgtgacatt tgcttctatt
    20761 tatcggcgta agaaagcgat gctgacgctc ggtcaggctg cgctggaaga tgcctgggaa
    20821 tatatggcac cgcgagagtg tgcgctgctt atcctcgatg aaaccgcctg cattctcagc
    20881 cgtaatggcg atccgcaaac cttgcagcag ctaagtgcac tgggattcaa tgacggcacg
    20941 tattgcgccg agggaattat tggtacttgt gcgctatcgt tagcggctat ctctggtcag
    21001 gccgtgaaaa cgatggccga tcaacatttc aaacaggcac tctggaactg ggccttttgt
    21061 gcaacgccgt tgtttgacag caagggccga ttgacgggaa caatagcgct ggcgtgtccg
    21121 gttgagcaaa ctaccgcagc tgatttgccg ttgacgttgg caatcgcccg cgaggtcggt
    21181 aatttactgc tgacggacag tttgctcgct gaaactaacc gtcatttaaa tcaacttaat
    21241 gccctgttag aaagtatgga tgatggcgtg attagctggg acgagcaggg taatttgcaa
    21301 tttattaatg cccaggcggc gcgggtcttg cgccttgacg cgacggcaag tcagggacgg
    21361 gcaatcactg aactcttaac gttacccgcc gtattgcaac aagcaataaa acaggcacat
    21421 ccgctcaaac acgtagaagc aacctttgaa agccagcacc agtttattga tgcggtgata
    21481 acccttaaac cgataataga aacgcaggga accagcttta ttttgttgct ccatcctgtg
    21541 gaacagatgc ggcagttgat gaccagtcaa ttaggaaaag tcagccatac ctttgctcat
    21601 atgccacagg acgatccgca aacccgccgc ttgattcatt ttggtcgcca ggcggcgcgc
    21661 agtagctttc ctgtcctgct ttgtggagaa gagggcgtgg gcaaggcact gctaagtcag
    21721 gcaattcata atgaaagcga gcgtgctgca ggtccttata tcgccgtcaa ttgtgagtta
    21781 tatggtgatg ctgcgctggc ggaagaattt attggtggcg atcgcacgga caatgaaaat
    21841 ggccgtctga gtcggctgga actggcacac ggcggcacgc tgtttcttga aaagattgaa
    21901 tatctggcgg tggagttaca gtctgctttg cttcaggtta tcaagcaggg ggttatcacg
    21961 cgactggatg cgcggcgttt aataccaatt gatgtcaaag tgattgcaac aacgaccgcg
    22021 gacctcgcaa tgctggtgga acaaaatcgt tttagtcgcc agctgtatta cgcgctgcat
    22081 gcatttgaaa ttaccatccc gcctctgcgt atgcggcgtg gcagcattcc ggcgctggtg
    22141 aataacaaat tacgcagtct tgaaaaacgc ttctctacgc ggctgaaaat tgatgacgat
    22201 gccctcgctc gcctggtttc ttgtgcatgg ccaggcaacg attttgaact ttacagcgtc
    22261 atcgagaatc ttgctctgag tagtgataac gggcgcattc gcgtcagtga tttacctgaa
    22321 catctgttta ccgagcaggc gacagatgat gtcagcgcca cccgcctttc caccagtctg
    22381 tcatttgcgg aagttgaaaa agaggcaatt attaacgcag cccaggtcac aggcggtcgc
    22441 attcaggaaa tgtcggcttt acttgggatc ggccgcacta cgctgtggcg gaaaatgaag
    22501 caacatggca ttgatgcagg gcagtttaag cgccgggtat gaaagacaga aacgatttct
    22561 gatacatcag agtgatctgt atttcattcc ggcgcacgct aacaattttc agcatcgttt
    22621 aagggcttgt ctatcccgca cttaaaagct gaagcgatat cctccgttga cttgcttctg
    22681 atcaaagagg ttaccctgcg tgtaatccgc ttcgagatag aatgtgtgct gtttgttata
    22741 ctgtgcactg actcccacgc cgttattcca gccattacct ttgaaactgt acttctcccg
    22801 ggagttgttc acagatattc ggtatcccct gaaaactcgc ggatagcgcc agtcttcaca
    22861 tagacattca gctggctgtt gcctgcggtg atgtcatacc ccagtatcat gctggcacgc
    22921 cccagcagcg attcgtagtg attcagatgt atattgaggc cattactcgc cttcatagcc
    22981 atctcattct ggtggctgta tgtaagctgg gtttgcggct ctatatagaa cccataacca
    23041 gtaggggaca ggttgaacct ctgcccggct tccagggaga tgctcattcc attcgcagtg
    23101 ccgttggcgt taacgccgtt gttctgactg tccagtacgt ggaaactatt tttctggcgc
    23161 gatgctttta taacgagatc gctgtaaaaa ccgttttgtg ccatgtaact ggcgtacatt
    23221 cccatgtagt ctgaacgtgc ggtaccgtcg cctccgctat agtccggcga tgcatgtgtt
    23281 gagccaatat acagaccgac atacaacggc attacatcag agagacgttt atccccacca
    23341 aactggatac cgctgtaacc catgtcaaag ccgctcagtt tgccactggc aaaggagtcc
    23401 aggcttcccc cataactgcg caaccagata ttaccgtctt tactctgatt tcgcaggtca
    23461 cccatccgtt gcatcagcgt acggttttca acatagttca ataagtaacc gacattgaga
    23521 taattgccgc cagcatctgc ggtcgtggtg ggtttaggcg tgggagcggg ttcaggcgta
    23581 ggatcggggt tgactatggg aggctgagcg ggagctggtg tgggttcagg attaggagtc
    23641 ggttccggaa ctgtcccgga agcgtaaagc tcccagttag tgccattttt acgcacatcg
    23701 tacagatatc cccccaactc aacctgagaa gacgcgctga acgaggccgc accgtcagtg
    23761 gtttttacca ctgtcagaac ttcatttccc gtagtggcct cgctgccctg gttgcggata
    23821 gccaatacgt gattaccagc actacttccg ctgatattca ataaatcccc tttattatta
    23881 acgccattac cctcgccaac aacatcagca cgcataataa aggtactgtt accgctcagg
    23941 ttctctacgt ttaatgtggc aaatgtgccg gcagttgacc cgtggctggc aaaatcgaca
    24001 gttgaatggc tcagcgccag cgtgtcgaga ttagaattac ttgttacgtt ccagacgctg
    24061 ttattcattg caacgtccag tttcccgcca ttgacattat cgctgaggga ggaacctgtc
    24121 caaaccgacc ctgggtgcat atctagattg attagcccac ctttggaaag aacgctaccg
    24181 ttgataagca tacgacccga ggcgttgatg cggctggcgg cataaccatc gtcatgttgc
    24241 gttgcaatgg ccatctggtc gggtgtactc atatcaatga ccagatcgct cgtgaggtcg
    24301 atctggctgt tggtcatggc ataaattccc ctggctcctg ccgcgccggt gatagccaaa
    24361 ctgtctccgg ttattctacc gccgctgagc gcccacaaac ccagcgccag actgccatta
    24421 cgatcaactg taatatcggt attttgcatg ttgacaacag ccgttgccgt ctgggccgag
    24481 gcaccataag aaccgccgga aaagatgctg tttcgttgcg ctgccgtgcc agaaaaattg
    24541 attgtcgcgt ctgaaccact ggtgacgagg ccaccgccct gcgcggaaga aatatggcta
    24601 tctgcaccga tagtggttgt accaccacga acttcgacgc cattggccgc ggctccagtt
    24661 acatcaacag tgagtgcatt cgcgctaacc tggccaaagc tccagaggcc gtgtgcatta
    24721 tcgccattgg ttttaatggt actgtttgtt ccgagatcga caacagagtt tttctgtacg
    24781 tttatcccca tggcgctgta gccctgaaca tcgattgtca ggtctgtcgc cgtaaaacgc
    24841 gcagcaccat tggcgttatt gccgttgaga ccaccgatat aaacacctgt acttccatcg
    24901 gtcgtgattt tacttccgct tccaagatcg acactggtgc catagtcatt gatggttagg
    24961 cctataccgt tcgagttttc aatggtgaat tgagtggctg ttagtgttga gctatgccca
    25021 ataatgatgc cgtcatcgtt actcttaatg gtgctgcctg tgcctaagtc agcatgggta
    25081 tagtcaccaa ttaagttaat gccgatggca gaggtttgac caacaacatc tactgtgagt
    25141 cggttagctg tcagggagct gtttttgccc tgaataatga ttccttttgc atatccagag
    25201 gcgtcgttga cattgacggt gacatcatta cccaggttga tattgccagg cgtgtttcct
    25261 gctggggtca ttacaccata gaggtttcct gatggatcgg cggtatctcc tgtaattctg
    25321 tcaccatcat taatagtaat attggtgcca tattgacttc ctgtatcagc agctatcagc
    25381 aggggggaaa agataattgc attcaaacca atagcgccaa attttatcag ttgaattgag
    25441 ggcgaacgga tttttaattc agcgagacga tctgctttgg tattcccatt gtgttgtttg
    25501 atgcccatac cactcctata tagtacccag actatgggtc tatggatttt gtcattttgt
    25561 ttaaatcaat gaataatatc ctcttatcat catgatattt gtatggtgca atcatgatga
    25621 gcttctcaga gaataattct ctattaccgg gcaaaatcag gaacacagga attaatatat
    25681 atttaacatc ccccttacaa aggtgggttt atatcgagtt attccctaag tt
//
LOCUS       DAZFXT010000063        25730 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.063, whole genome shotgun sequence.
ACCESSION   DAZFXT010000063 DAZFXT010000000
VERSION     DAZFXT010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25730
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.063"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..773
                     /gene="adk"
                     /locus_tag="ACHKW0_002793"
     CDS             129..773
                     /gene="adk"
                     /locus_tag="ACHKW0_002793"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004017 - adenylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HFO7475598.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            1009..1971
                     /gene="hemH"
                     /locus_tag="ACHKW0_002794"
     CDS             1009..1971
                     /gene="hemH"
                     /locus_tag="ACHKW0_002794"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HFO7475599.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(1968..2927)
                     /gene="aes"
                     /locus_tag="ACHKW0_002795"
     CDS             complement(1968..2927)
                     /gene="aes"
                     /locus_tag="ACHKW0_002795"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HFO7475600.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            3079..4383
                     /gene="gsk"
                     /locus_tag="ACHKW0_002796"
     CDS             3079..4383
                     /gene="gsk"
                     /locus_tag="ACHKW0_002796"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HFO7475601.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(4516..6192)
                     /gene="ybaL"
                     /locus_tag="ACHKW0_002797"
     CDS             complement(4516..6192)
                     /gene="ybaL"
                     /locus_tag="ACHKW0_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaL family putative K(+) efflux transporter"
                     /protein_id="HFO7475602.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGLVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(6430..7650)
                     /gene="fsr"
                     /locus_tag="ACHKW0_002798"
     CDS             complement(6430..7650)
                     /gene="fsr"
                     /locus_tag="ACHKW0_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HFO7475603.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKSKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            7868..9520
                     /gene="ushA"
                     /locus_tag="ACHKW0_002799"
     CDS             7868..9520
                     /gene="ushA"
                     /locus_tag="ACHKW0_002799"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HFO7475604.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDVEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(9557..10036)
                     /gene="ybaK"
                     /locus_tag="ACHKW0_002800"
     CDS             complement(9557..10036)
                     /gene="ybaK"
                     /locus_tag="ACHKW0_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HFO7475605.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(10240..11034)
                     /gene="ybaP"
                     /locus_tag="ACHKW0_002801"
     CDS             complement(10240..11034)
                     /gene="ybaP"
                     /locus_tag="ACHKW0_002801"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HFO7475606.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            11172..11513
                     /gene="ybaQ"
                     /locus_tag="ACHKW0_002802"
     CDS             11172..11513
                     /gene="ybaQ"
                     /locus_tag="ACHKW0_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7475607.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(11728..14232)
                     /gene="copA"
                     /locus_tag="ACHKW0_002803"
     CDS             complement(11728..14232)
                     /gene="copA"
                     /locus_tag="ACHKW0_002803"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HFO7475608.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAVDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            14494..15426
                     /gene="glsA"
                     /locus_tag="ACHKW0_002804"
     CDS             14494..15426
                     /gene="glsA"
                     /locus_tag="ACHKW0_002804"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HFO7475609.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPSQLAAVA
                     IVTCDGNIYRAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHERVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVANQLGYNVFKG"
     gene            15429..16721
                     /gene="ybaT"
                     /locus_tag="ACHKW0_002805"
     CDS             15429..16721
                     /gene="ybaT"
                     /locus_tag="ACHKW0_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HFO7475610.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            16752..16849
                     /locus_tag="ACHKW0_002806"
                     /pseudo
     CDS             16752..16849
                     /locus_tag="ACHKW0_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308571.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            16846..17253
                     /gene="cueR"
                     /locus_tag="ACHKW0_002807"
     CDS             16846..17253
                     /gene="cueR"
                     /locus_tag="ACHKW0_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HFO7475611.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(17254..17712)
                     /gene="ybbJ"
                     /locus_tag="ACHKW0_002808"
     CDS             complement(17254..17712)
                     /gene="ybbJ"
                     /locus_tag="ACHKW0_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HFO7475612.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(17709..18626)
                     /gene="qmcA"
                     /locus_tag="ACHKW0_002809"
     CDS             complement(17709..18626)
                     /gene="qmcA"
                     /locus_tag="ACHKW0_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HFO7475613.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            18772..19449
                     /gene="fetA"
                     /locus_tag="ACHKW0_002810"
     CDS             18772..19449
                     /gene="fetA"
                     /locus_tag="ACHKW0_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HFO7475614.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            19436..20215
                     /gene="fetB"
                     /locus_tag="ACHKW0_002811"
     CDS             19436..20215
                     /gene="fetB"
                     /locus_tag="ACHKW0_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HFO7475615.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDIFWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(20278..21132)
                     /gene="cnoX"
                     /locus_tag="ACHKW0_002812"
     CDS             complement(20278..21132)
                     /gene="cnoX"
                     /locus_tag="ACHKW0_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HFO7475616.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(21193..22002)
                     /gene="ybbO"
                     /locus_tag="ACHKW0_002813"
     CDS             complement(21193..22002)
                     /gene="ybbO"
                     /locus_tag="ACHKW0_002813"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HFO7475617.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNNMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(21992..22615)
                     /gene="tesA"
                     /locus_tag="ACHKW0_002814"
     CDS             complement(21992..22615)
                     /gene="tesA"
                     /locus_tag="ACHKW0_002814"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016298 - lipase activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HFO7475618.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            22586..23272
                     /gene="ybbA"
                     /locus_tag="ACHKW0_002815"
     CDS             22586..23272
                     /gene="ybbA"
                     /locus_tag="ACHKW0_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter ATP-binding protein
                     YbbA"
                     /protein_id="HFO7475619.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLGHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            23269..25683
                     /gene="ybbP"
                     /locus_tag="ACHKW0_002816"
     CDS             23269..25683
                     /gene="ybbP"
                     /locus_tag="ACHKW0_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit YbbP"
                     /protein_id="HFO7475620.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGSENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLLLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHQIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHLELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         6100 a         6776 c         6893 g         5961 t
ORIGIN      
        1 cactttccgc aaattatctc gccattaacc gtttcagcct caggtgcctt tcttgaggca
       61 atcgcctgtt ggtggtatcg tttatcgctt tttcaaaaaa ttcgacacat tttaagggga
      121 ttttcgcaat gcgtatcatt ctgcttggcg ctccgggcgc ggggaaaggg actcaggctc
      181 agttcatcat ggagaaatat ggtattccgc aaatctccac tggcgatatg ctgcgtgctg
      241 cggtcaaatc tggctccgag ctgggtaaac aagcaaaaga cattatggat gctggcaaac
      301 tggtcaccga cgaactggtg atcgcgctgg ttaaagagcg cattgctcag gaagactgcc
      361 gtaatggttt cctgttggac ggcttcccgc gtaccattcc gcaggcagac gcgatgaaag
      421 aagcgggcat caatgttgat tacgttctgg aattcgacgt accggacgaa ctgattgttg
      481 atcgtatcgt aggccgccgc gttcatgcgc cgtctggtcg tgtttatcac gttaaattca
      541 atccgccgaa agtagaaggc aaagacgacg ttaccggtga agaactgact acccgtaaag
      601 acgatcagga agaaaccgtg cgtaaacgtc tggttgaata ccatcagatg actgcaccgc
      661 tgatcggcta ctactccaaa gaagcggaag cgggtaacac caaatacgcg aaagttgacg
      721 gcaccaagcc ggttgctgaa gttcgcgctg atctggaaaa aatcctcggc taattcgaaa
      781 gcgcgcacgg acagtcccct cgccccctcg gggagagggt tagggtgagg ggaacaggcc
      841 cgcacaagca aacttatcag caatctcagg ccggatattc attcggcctt ttacaaaaac
      901 ctcaatctgc gctattcctt tttctgattt gacctctcac agcaattagt tcttcttcct
      961 cacttttccg ctacaattat caacaagttg aatcgataag aggtggtaat gcgtcagact
     1021 aaaaccggta tcctgctggc aaacctgggt acgcccgatg cccccacacc tgaagcggta
     1081 aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc acggttgtta
     1141 tggtggccgt tgctgcgcgg cgtgattttg ccgctgcgct cgccgcgtgt ggcgaagctg
     1201 tacgcctcgg tctggatgga agacggctcg ccgctgatgg tttacagccg ccagcaacag
     1261 caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggcatgag ctacggctcg
     1321 ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca tattgtggtg
     1381 ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga tgaactggca
     1441 cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga ttacgccgat
     1501 aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc caaacatggc
     1561 gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc agatgaaggc
     1621 gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc actggggatg
     1681 gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc ctggctgatg
     1741 ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca tatacaggtg
     1801 atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc cgagcaaaac
     1861 cgtgaggtct tcctcggtgc cggcgggaaa aaatatgaat atattccagc gcttaatgcc
     1921 acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta aagctgagcg
     1981 gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat tcgtgaataa
     2041 tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg ctgatgcgcc
     2101 gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc cgccccggca
     2161 ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta atacggtgac
     2221 tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc ctgttgcgtt
     2281 aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa tccgtaaagc
     2341 ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc acgcaaccac
     2401 aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc aatgcgggac
     2461 atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc cacaatttcc
     2521 tctatcgctt gcggaaaacg cgcttcaggt gaaagggggt aatcaatacc aatcaccgta
     2581 cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag attgccgaga
     2641 ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg cggacaaaag
     2701 agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt tgccatttct
     2761 ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg ctcagcaatc
     2821 gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac ggtcttcatt
     2881 tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca aaaactcctt
     2941 tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc tgctgatatt
     3001 gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc gctcagttaa
     3061 ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca ttacttccct
     3121 gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac cagcgccgcc
     3181 tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga tgatgaattc
     3241 attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga cgtagccgaa
     3301 gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc gggtggcacc
     3361 attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt gctgctgggc
     3421 gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg taacacttcc
     3481 agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg ttgctttacg
     3541 ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat gaaccagctg
     3601 cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt tctcacctct
     3661 tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa agccattgag
     3721 tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt tgtcattgcc
     3781 gagaatccgc agtggtggca gcaattcctc aaagaccacg tttctatcct tgcgatgaac
     3841 gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc tgacaaggcg
     3901 ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta tatggcgggc
     3961 tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg cgctatagcg
     4021 gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca gaatccgctg
     4081 cgtgtatatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat gaacactaat
     4141 ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa cagctaccat
     4201 cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta ttcatcgtta
     4261 gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca gcattcacct
     4321 cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta ctgggatcgt
     4381 taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg catttggctc
     4441 tcgatgcccg atgcgacgct ggcgcgtctt atcatgccta catatttttc atattttaca
     4501 tccggcaacc accgtttacc ccgtcaccac ttcacctgcc ggtggcgttt ccagcagttc
     4561 cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc cacgttcggt
     4621 gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat ccgggttttt
     4681 cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg tcaggatcag
     4741 ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg cattgcccaa
     4801 tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg tctcaatcac
     4861 caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta cacgaccgta
     4921 acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt cttcgattgc
     4981 ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct ccagtagtgc
     5041 gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca ggttttgtcc
     5101 ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg cgaactcacc
     5161 aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac caaacagtcg
     5221 caccaggaaa aatgcggcta acgacttacc aaacagaata atcgccagcg tcgccagcac
     5281 tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg agacaaaaaa
     5341 cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt gactcagttc
     5401 agactcgttc agtaccatcc cggcaaagaa cgcaccgagt gcaaaggaga catcaaacag
     5461 ctctaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg taaacagctc
     5521 gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc gacctaccag
     5581 catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat cgactgcaag
     5641 agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca gcaacaccag
     5701 cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt gcccacgctg
     5761 actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac tggcggtgga
     5821 aagacataaa ccgaacacga gaccggtcat taacgaccag cccagcacgg cagagagcgc
     5881 catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg cgatggcctt
     5941 taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca acagaatgac
     6001 gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg gagtgaatgg
     6061 tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta gtttattggc
     6121 cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga taagcggggt
     6181 ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg ccgggaaaac
     6241 caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa taattattga
     6301 attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac agaaggcggt
     6361 ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt gcctttggct
     6421 gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca tccccaatag
     6481 tggcaggaaa gcacagattt tatagactaa ctcgatgctg gtgtgatcgg cgataagccc
     6541 cagaactgcc gctcccagac ctcccatgcc aaaagcaaaa ccgaaaaaga gtccagaaac
     6601 cataccgata cgtcccggaa gtagctcctg agcgtagacc agaatggcag agaatgccga
     6661 agcgaggata aatccaataa tcaccgttaa aacccccgtc cagtgcaggc tggcgtaggg
     6721 taaaatcagc gtaaacggcg caacgccgag gatagagccc caaatcacat atttccgccc
     6781 aattttatcc cctacaggcc cgccgatcac cgtacctgcc gcaacggcaa acaggaaggc
     6841 aaacagatga agctgggcat tctggataga taatccgaat ttttgcatca gataaaaggt
     6901 gtaatagctg ctgatgctcg ccatatagaa atatttcgag aaaatgagga ttaacagaat
     6961 gctgactgcc agtaccactt tgttgcgcgg cagtggattg attatcgtcg ctttggattt
     7021 tcctttattc attcggtgct gtgccgagta ccaacggcta atttgcgcca acaccacgat
     7081 cgccagcagt gccgcaagca caaaccaggc aacgttgcct ttgccataag gcgcgataat
     7141 caccgccgcc agcaagggtc ccagggaact gccaaagttg ccgccgacct gaaagataga
     7201 ttgcgccagg ccatgccgcc cgccggaagc catacgggct acgcgagaag attccggatg
     7261 aaagaccgat gaaccggtac cgaccagcgc cgccgccagc agaactgcgc caaaactgcc
     7321 cgccagcgca agcagcacca gaccacttaa ggtaaagcac atgccaattg gcaacgacca
     7381 cggcatcgga tatttatcgg tccagtagcc gaccactggt tgcagtagcg aagaggcgag
     7441 ctggaaggtg agggttatca tgccaatctg cataaatgtc agagaaaatt ctgactgaag
     7501 cagcggataa atcgccagaa tcagcgattg gatcatgtcg ttcagcagat gtgagaggct
     7561 gatagcacct aaaataccaa acgatgttcg ggccttggtc gttgacgcag ccgcgcccgc
     7621 cacaggctgg ggttgttcac tcattgccat aggaaagtca ctttttcagg gttgcgatgt
     7681 aaagaatgat cttatttgtg attattaccc gactaacata cctgtatgcg tcgcctgaag
     7741 gaagtctcaa cgccgaatac agaatttcta atctggatgc agatttatct tcaccggacg
     7801 cagacttgtc tatgatgtcg cgtcatacta tttttcaaca cgttgaaatc aggtcaggga
     7861 gagaagtatg aaattattgc agcggggcgt ggcgttagcg ctgttaacca catttacact
     7921 ggcgagtgaa actgctctgg cgtatgagca ggataaaacc tacaaaatta cagttctgca
     7981 taccaatgat catcatgggc atttttggcg caatgaatat ggcgaatatg gtctggcggc
     8041 gcaaaaaacg ctggtggatg gtatccgcaa agaggttgcg gctgaaggcg gtagcgtgct
     8101 gctactttcc ggtggcgaca ttaacactgg cgtgcccgag tctgacttac aggatgccga
     8161 acctgatttt cgcggtatga atctggtggg ctatgacgcg atggcgatcg gtaatcatga
     8221 atttgataat ccgctcaccg tattacgcca gcaggaaaag tgggccaagt tcccgttgct
     8281 ttccgcgaat atctaccaga aaagtactgg cgagcgcctg tttaaaccgt gggcgctgtt
     8341 taagcgtcag gatctgaaaa ttgccgtcat tgggctgaca accgatgaca cagcaaaaat
     8401 tggtaacccg gaatacttca ctgatgtcga atttcgtaag cccgccgatg aagcgaagct
     8461 ggtgattcag gagctgcaac agacagaaaa gccagacatt attatcgcgg cgacccatat
     8521 ggggcattac gataatggtg agcacggctc taacgcaccg ggcgatgtgg agatggcacg
     8581 cgcgctgcct gccggatcgc tggcgatgat cgtcggtggt cactcgcaag atccggtctg
     8641 catggcggca gaaaacaaaa aacaggtcga ttacgtgccg ggtacgccat gcaagccaga
     8701 tcaacaaaac ggcatctgga ttgtgcaggc gcatgagtgg ggcaaatacg tgggacgggc
     8761 tgattttgag tttcgtaatg gcgaaatgaa aatggttaac taccagctga ttccggtgaa
     8821 cctgaagaag aaagtgacct gggaagacgg gaaaagcgag cgcgtgcttt acactcctga
     8881 aatcgctgaa aaccagcaaa tgatctcgct gttatcaccg ttccagaaca aaggcaaagc
     8941 gcagctggaa gtgaaaatag gcgaaaccaa tggtcgtctg gaaggcgatc gtgacaaagt
     9001 gcgttttgta cagaccaata tggggcggtt gattctggca gcccaaatgg atcgcactgg
     9061 tgccgacttt gcggtgatga gcggaggcgg aattcgtgat tctatcgaag caggcgatat
     9121 cagctataaa aacgtgctga aagtgcagcc attcggcaat gtggtggtgt atgccgacat
     9181 gaccggtaaa gaggtgattg attacctgac cgccgtcgcg cagatgaagc cagattcagg
     9241 tgcctacccg caatttgcca acgttagctt tgtggcgaaa gacggcaaac tgaacgacct
     9301 taaaatcaaa ggcgaaccgg tcgatccggc gaaaacttac cgtatggcga cattaaactt
     9361 caatgccacc ggcggtgatg gatatccgcg ccttgataac aaaccgggct atgtgaatac
     9421 cggctttatt gatgccgaag tgctgaaagc gtatatccag aaaagctcgc cgctggatgt
     9481 gagtgtttat gaaccgaaag gtgaggtgag ctggcagtaa tccgaaagtg ccggatgttt
     9541 gcatccggca caatgcttaa tcgcggcggg cgatatcagc aaatttggca tcgaggatct
     9601 ttgccagatc gcctgccgcc agttcgatat ccagtccgcg cttgccgccg gaaacataaa
     9661 tagtggcaaa ttcttgtgcg ggggcgtcga taatcgttgg cagacgtttt ttctgcccca
     9721 gtgggctaat ccccccaacc aggtatcccg tcgaacgctg cgcgaccatc ggatcggcca
     9781 tctcaacttt cttggcaccc agcgcttttg ctactttttt aagatccagt tgacctgcga
     9841 ccggcgtaac ggccacggca aggtgtttca tatcaccgtt cactgccacc agcagcgttt
     9901 tgtagacctg atccggattc aaacctaatt ttttgacgac ttcatcgcca aaattggttt
     9961 cagccggatc gtgctcgtag gtatggattt gaaacgaaat cttgtttttt tcgagtaatt
    10021 taactgcggg tgtcatggta attttcctgc cttaaacaaa aaaacgaagc aagcatacgc
    10081 ctgatggcgg tccaaaaaaa agagtcatct tgcctaagag tattggcagg atggtgagat
    10141 tgagcgacaa tcgagttaca ccgtcgctta aagtgacggc ataataataa aaaaatgaaa
    10201 ttcctctttg acgggccaat agcgatattg gccatttttt tagcgcaaca tttgcggcaa
    10261 attcccttct ccatacaggt gtagtgcacc gaccgcgacc acatatcgcc ccggcggcat
    10321 ggcgcgtaat ttatcccgcc aggcgagatt tcgctgatgc atcagcacat cgtacagcga
    10381 ctgactgaac gtattgggca gcgttatatc attattttgc ggcggtgcat tcagccacca
    10441 gctcatcatt tgttgcagca accgtgcgtt ggtatgccag tgggtcagcg tatcgtccag
    10501 cagcgccagt cctttgtcag ggagctggag caacatggca atctggtttt cagccccttc
    10561 cagttcaatc acgggtttat gttgttgctt cgccgcctgc aataactggt aatcgatacc
    10621 gtattctgcc cgcagcccca gtttttgtgc ctgcgtcgcc tgaagaacca tcgcgatttg
    10681 ccacagcggt tgagtagaaa agagcgacgg agaaatgccc atctcctggc taatgtgctg
    10741 cagattttgg agttgctcct cgttaatgcg ctcttccagc gcctcgcaag taggcaaatt
    10801 agcaaaaggc gtatcgctgg tggaaacatc cgcctcgacg atcagcgcat cggcgttttt
    10861 gagctttttg agcaaacggg tgggcagggg agccatatcg tggctaccca tatgaatact
    10921 gccaatcaga tgaaaatggc gattgccggg aagggtgata tcgatggcag gccaggtgta
    10981 atgattaccg cgcagcgcgg cccaaagcgt ttttacccgg tacaacagat ccatacgacc
    11041 tccctttgtg aaatatcatg ctagcgcgcg gtgagggatg gcgcaaatgg agttggggac
    11101 ttgtttaatg tgtttgtatg attcagtatg ttcttgcatc gctattcaca gggaagcaac
    11161 agttaaaaac catgaaacag gcaacaagaa aaccgacgac acccggagat attcttctct
    11221 atgaatatct ggaaccgctc gatttgaaaa tcaatgagtt agcagagttg ctgcatgttc
    11281 atcgtaatag cgtcagtgca ctgatcaata acaatcgtaa actcaccact gagatggcat
    11341 tccgtctggc gaaagttttt gataccacag tcgattttag gctaaacctc caggcggcag
    11401 ttgatctttg ggaagttgaa aacaacatgc gcacccagga agaattggga cggattgaaa
    11461 cagtggctga atatttggca cgccgtgaag agcgtgcaaa aaaggtcgcg taaggccaaa
    11521 acatcgcttc atctcgtagg ccggataagg cgttcacgcc gcatccggca tccgagcacc
    11581 attgcctgat gcgacgctta cccgtcttat caggcctaca aacctgtgcc tgaaccgtag
    11641 gccggataag gcgtttacgc cgcatccggc agtcatgcgt cgatgccaaa tgcgccgccc
    11701 taaagcagcg catccgcaat gacgtactta ttccttcggt ttaaaccgca gcaaccggtt
    11761 ggcgttactc actacggtaa tcgacgagag cgccattgcc gctccggcaa ctaccgggtt
    11821 aagcagtgtt ccagtaaacg gccacaaaat accggcggcg accggaatac cgatgctgtt
    11881 gtagataaag gcaccgagca ggttctgctt catgttgcgc agcgttgcgc gggaaatcgc
    11941 gagcgcatcc gcaacgccca tcaggctatg gcgcatcagg gtaatcgccg cggtttcaat
    12001 ggcaacatca ctgccgccac ccatcgcaat gccgacatcc gcctgggcca gcgctggcgc
    12061 gtcgttaatg ccgtcgccca ccattgccac ctgacgtcct tcactttgca ggcgtttaat
    12121 cgcttcggct ttaccgtccg gcagcacccc ggcgatcact tcatcaatcc cggcttcttt
    12181 ggcgatcgca ttggcagtgg ttgggttatc cccggtcaac atcaccagac gatatcccgc
    12241 tttatgcagg cgttgcagcg ccgccacgct atcactacgc aatggatcgc gtactgccag
    12301 cagggctacc gctttcccgt caaccgccag cagcacaggc gttgcccctt gcgatgcctg
    12361 agcagtaatc tccgcttcga tagctttggt accaacctgt tgctcattta acagcgcctg
    12421 attgcccagc aataacgcat gaccttcagc ttcaccgctc acgcccagcc cgcgcaatgt
    12481 gcggaaaccg ttgacctgcg gcagctgcat atcacctgct ttatcgagga tcgcccgtgc
    12541 cagcggatgg ctggaacctt gctccagtgc cgccgccaga cgcaatgcct gcgcttcatc
    12601 aacatcagca aacgttttca ctgcgacaac ctgcggcttc ccttcggtca gcgtcccggt
    12661 tttatcgaac acgacggtat cgagtgtact ggcgcgttgc agcgcgtcgg cgtcccgcac
    12721 cagcacgcca aactcagccg cccgcccgac gccggaaata atcgacatcg gcgtcgccag
    12781 ccccagcgca cacggacagg cgataatcag taccgtggtg gcaatcacca gggtataaac
    12841 aatttgcggt gccggaccaa agaagtacca gattgccgca ctgaccagcg caatcaccac
    12901 caccacaggt acaaatacgg ctgagatttt atccgccagc tgaccgattt ctggcttgct
    12961 gctctgggcc tggcgcacca tgcgaatgat ccgtgacagc gtggtatggc tgccaaccgc
    13021 actggcacga aacagcacgc taccgtcctg taccactgtc ccggcatgga cgctttcgcc
    13081 ttcgcctttt tgctgcggga ttggttcgcc cgtcagcatc gcttcatcca gccatgcttc
    13141 gccctgggta atctcgccat ctaccggcac gcgatcgccg gtcgtcaggc gcagcaacat
    13201 acctggctgc acttccgcca gaggcacgct tttttcacct tcgtcagtaa ccaggcgcgc
    13261 cgtcggtggg gttaaatcga gtaatttctc cagcgcctta gaagaacgct gacgtgcgcg
    13321 tgcttccagc atatggccga gattgatcag accgataatc atcgcgctgg cttcgtaata
    13381 aagatgtcgc gcttccatcg ggaaccactg cggccacagg ttgacgctca tcgaatagag
    13441 ccacgccacg ccagtaccca gcgccaccag cgtatccatc gtcgctgcac cgttcagcag
    13501 gcttttccat gcactgcggt aaaaatggcc gccggcgaaa accatcaccg ccagggttat
    13561 caggccgata accaaccaca ggctgcggtt gtcagcggtg accatcatgt tatcgccaat
    13621 catcccccag accatcaccg ggatacccac cgccagtgcg acaattgcct gccagcggaa
    13681 gcgcttcatc gtagcaacgg cggtttcctg ctggcgctca cggcgtttcg cgtcatcttc
    13741 aatcgcttcc gcgccgtagc ccgctttttc caccgcctgc actaaatctt gtggggaggc
    13801 actgcccatc accagcgcag tacgctccgc caggtttacc cgtgcctgag tgacgcccgg
    13861 tacgctttgc agcgcatttt gtacgcgggt gacacagctg gcgcagctca tgccgctcag
    13921 cagcaactgc tggctgtcat cgtcatcggc ggtcgctgcc ggaagcgcct cagaaaccgc
    13981 tgtcagtgct tccgacggga ttgatgactc cgccagcggt ttagcctttg ggtggcttac
    14041 agatgcgtca taacccgctt gtttgatggt ttcgatcagc tgttctgcac tggcagtccc
    14101 ggtaacgtgc gcttcagtga tagacacatc cgcttgttcc acatccgggc gctgttcgag
    14161 actttctttc acgcgtttaa cgcagtgacc gcaggacagg ccgtccaggg tcaggtcgat
    14221 agtttgtgac ataaaacact cctttaagac agttttgact ggctgtgata aaggttaaac
    14281 cttccagcaa ggggaaggtc aagaaattaa taaaccaggc gagtaaaagt ccgtaaagat
    14341 taaaaaattg gctcaatttg catcaggatt agacatttat ctctttgttt tcctgtagtt
    14401 aagttgcggg tgctaagtta aatcaagatg cctgaaaatc ggcaccgggg tgaggaatta
    14461 cctcccgcat ctataaaaag gagttaacaa aagatgttag atgcaaacaa attacagcag
    14521 gcagtggatc aggcttacac ccaatttcac tcacttaacg gcggacaaaa tgccgactac
    14581 attccctttc tggcgaatgt acctagtcaa ctggcagcag tggctattgt gacctgcgat
    14641 ggcaacatct atcgcgcggg tgatagtgat taccgctttg cgctggaatc catctcgaaa
    14701 gtctgtacgt tagcccttgc gttagaagat gtcggcccgc aggcggtaca ggacaaaatt
    14761 ggcgctgacc cgaccggatt gccctttaac tcagttatcg ccttagagtt gcatggcggc
    14821 aaaccactgt cgccactggt aaatgctggc gccattgcca ccaccagcct gattaacgct
    14881 gaaaatgttg aacaacgctg gcagcgaatt ttacatatcc aacagcaact ggctggtgag
    14941 caggtagcgc tctctgacga agtcaaccag tcggaacaaa caaccaactt ccataaccgg
    15001 gccattgcct ggctgctgta ctccgccgga tatctctatt gtgatgcaat ggaagcctgt
    15061 gacgtgtaca cccgtcagtg ctccacgctc ctcaatactg ttgaactggc aacacttggc
    15121 gcgacgctgg cggcgggggg tgtgaatccg ctgacgcatg aacgcgttct tcaggccgac
    15181 aacgtgccgt acattctggc cgaaatgatg atggaagggc tgtatggtcg ctccggtgac
    15241 tgggcgtatc gcgttggttt accgggcaaa agcggtgtag gtggcggtat tctggcggtc
    15301 gtccctggcg tgatgggaat tgccgcgttc tcaccaccgc tggacgaaga aggcaacagt
    15361 gttcgcggtc aaaaaatggt ggcatcggtc gctaatcaac tcggctataa cgtgtttaag
    15421 ggctgatcat gatgaacacg gaaggtaata acggtaacaa acccctcggt ctatggaatg
    15481 tcgtttccat cggtattggg gcaatggtgg gggcggggat cttcgcgctg ctggggcagg
    15541 ctgcgttgct aatggaagcc tcgacctggg tcgcctttgc ttttggcggt attgtggcga
    15601 tgttttccgg ttatgcctat gcgcgtctgg gggcgagcta tcccagcaat ggcggcatta
    15661 tcgacttctt tcgtcgcgga ttaggcaacg gcgtcttttc gctggcgctc tcgttactgt
    15721 acctgttgac gctggcggtg agcatcgcca tggtcgcccg tgcttttggc gcttatgccg
    15781 tgcagttttt gcatgaaggc agccaggagg agcaccttat tttgctctac gcgttgggga
    15841 tcattgcggt gatgacgctt ttcaactcct taagcaacca tgcggtaggg cggctggaag
    15901 tgatcctcgt cggcattaaa atgatgatcc tgttattgct gattattgcc ggtgtctggt
    15961 cgctgcaacc ggcgcatatt tccgtctctg cgccccccag ctccggtgcg ttcttctcct
    16021 gtatcgggat aaccttcctt gcctatgcgg gctttggcat gatggcgaac gcggcggata
    16081 aagtgaaaga tccgcaggtc attatgccgc gggcgtttct ggtggcgatt ggcgttacca
    16141 cgttgcttta tatctcgctg gcactggttt tgcttagcga tgtatcggca ttagagttag
    16201 aaaaatatgc cgataccgcc gttgcgcagg ctgcttctcc gctgctcgga catgtgggtt
    16261 atgtgatcgt cgtcatcggc gctttactgg cgacggcttc agccattaac gcgaacctgt
    16321 tcgccgtgtt taacatcatg gacaacatgg gcagcgaacg cgaactgccg aagctaatga
    16381 ataaatccct gtggcggcag agtacctggg gcaacatcat tgtcgtggtg ttgattatgc
    16441 tgatgacggc ggcgctaaat ttaggctcac tcgccagtgt tgccagcgcc acctttttga
    16501 tttgctacct ggcggtgttt gtggtggcga tccgcctgcg tcatgatatt cacgcctcgt
    16561 tgccgattct tatcgttggt acgttggtga tgttgttggt gatcgttggc tttatctaca
    16621 gtctgtggtc ccagggtagc cgtgcgttga tatggattat tggctcactc ttactcagcc
    16681 ttattgtggc aatggtcatg aagcgcaata aaaccgtata acatctctct gtgcgcagta
    16741 cttcctgtat tattgtggtg gcggtcgata ttcgcactgg caaaaaaacg tgcttgaata
    16801 tctgttgaaa ccctttaaca aagcacagga ggcgttgcgc gaacgatgaa catcagcgat
    16861 gtagcaaaaa ttaccggcct gaccagcaaa gccattcgct tctatgaaga gaaggggctg
    16921 gtgacgccgc cgatgcgcag cgaaaacggt tatcgcacct acacgcagca gcatctcaac
    16981 gaactgacct tactgcgcca ggcacggcag gtgggcttta acctggaaga gagcggcgag
    17041 ctggtgaatc tgtttaacga cccgcagcgg cacagcgccg acgtcaaacg gcgcacgctg
    17101 gagaaggtgg cggagatcga acgacacatt gaggagctgc aatccatgcg cgaccagctg
    17161 ctggcactgg cgaatgcctg ccctggcgat gacagcgccg actgcccgat tatcgaaaat
    17221 ctctccggct gctgtcatca tcgggcaggg tgattaagac gagacggctc ggatatgtag
    17281 ggttatccct tctatcgcaa tgacttcaac atgcgtacct gcaccgagat cctcgctggc
    17341 gctgacaggc catgaactgt caccgacgcg catatgaccg cgcccgttga ccagcggaga
    17401 ttccagcaca aaacgtcggc caatcagctg ctgcccgcgc tggtttaaat gactatcgct
    17461 gtgcttttgt tcgcgcaccc gccgcgacaa ccatttccac cacagccagg cggcgagcag
    17521 cgtcaggatg gcaaacatta ccccttgcca ctcccaaccc agcggcacca gccagaccac
    17581 caggccggta atcaccgctg ccacgccgct ccacaacaaa taaccatttc cgcccagcat
    17641 ctcggctgcc agcagcaaac cgccgagact gagccagaaa atatgtggat gaacgaccat
    17701 taactccatc atggctgagt ccgtttgttg gcgctgtctt tcaccagctc ggcaatcccg
    17761 gcaatcgacc ccatcaggct gctggcctct aatggcatca tcactacttt gctgttactg
    17821 gaggaaccga tctgctgtaa cgcttcggtg tatttctgcg ctacgaagta gttcaccgcc
    17881 tgaatatcac cggaggcgat agcttcagac accattttgg tggcgcgggc ttctgcttcg
    17941 gcggaacgtt cacgcgcttc agcctgtaaa aacgccgact gacgttcgcc ttccgctttc
    18001 aggatttgcg actgtttttc accttcggct ttgaggattt ccgcctgacg gatcccttcc
    18061 gcttcaagaa tgtaagcgcg tttggtacgt tccgctttca tctgcgcgtt cattgaagag
    18121 ataagctctg ccggtgggcg cacgtcgcga atttcaatac gggtgacttt aatcccccac
    18181 gggttggtgg cttcatcgac aatacgcagc aggcgtgagt tgatgctgtc gcgctgagag
    18241 agcatttcgt caagttccat tgaacccaac acggtacgga tgttagtcat ggtcaggttg
    18301 atgatcgcca gctccagatt gctgacttca taagccgcgc gcggcgcgtc aatcacctga
    18361 ataaaacaga ctgcgtcgat ggtaacgttg gcgttatctt tcgagataac ttcctgggaa
    18421 gggatatcga gcacttgctc catcatattg atcttgcgac caatgcgatc cataaacggc
    18481 accaccagac tgagccccgg ctgtaacgtt ttggtatagc gaccaaaacg ttctaccgtc
    18541 cattgatagc cctgcggcac gattttgacg cccgcgccga caatgaccag cgcgacaaaa
    18601 atgagaatcg ggataaagat aagcatcgga aaaacctcct gttgtaccgt ccataatcag
    18661 caaaattgct gcttgattaa acaaattata cctgattact gaaagagagt tcccccttat
    18721 tcctgcgaag gataaactgt ttttagtaaa aatcagaaaa agggaacagt gatgcaggaa
    18781 aatagtcctt tgcttcagct acaaaacgta ggatatctgg cgggtgatgc gaagattctt
    18841 aataacatca atttttcgct gcgtgctggc gaatttaagt taattaccgg tccttccggt
    18901 tgtggcaaaa gtacgctgct aaaaatagtt gcttcattga tcagcccaac cagcggaacg
    18961 ttactgtttg aaggtgagga tgtcagcaca ctaaagccag aaatctaccg ccagcaagtc
    19021 tcttactgcg cccagacgcc gacgctgttt ggcgatacgg tatacgataa tctgatcttt
    19081 ccctggcaga tccgtaacca gcagcctgac ccggccattt ttctcgattt tctcgaacgc
    19141 ttcgctttgc cggacagcat tttgacgaaa aatatcgccg agctatctgg tggtgaaaaa
    19201 caacgcatct cactgattcg taacctgcaa tttatgccga aggttttatt gctggatgaa
    19261 atcaccagtg cgctggatga aagtaataaa cataacgtta atgagatgat ccatcgttat
    19321 gtgcgcgagc aaaatattgc cgtgctgtgg gtgacacacg ataaagacga aattaatcat
    19381 gcggataaag tgattacact gcaaccgcat gccggagaaa tgcaggaagc acgctatgaa
    19441 ctcgcataat attactaacg aatcattggc actggcatta atgctggtgg tggtggcaat
    19501 cttaattagc cataaagaaa aactggcgct ggagaaagat attttctgga gcgtcgggcg
    19561 agcgataatt cagctgatta ttgtcggcta tgtgctgaag tatattttca gcgtggatga
    19621 tgccagcctg acattattga tggtgttatt tatctgcttt aatgcggcgt ggaacgcgca
    19681 aaaacgcagt aaatatattg ctaaagcttt tatctcatcg tttattgcca ttacggtcgg
    19741 ggcgggaatt accctggcgg tgctgattct ctctgggtcg attgaattta tcccgatgca
    19801 ggtgatccct atcgccggga tgattgccgg taacgccatg gtagcggtgg ggttgtgtta
    19861 caacaattta gggcaacggg tcattagcga acagcaacag attcaggaga aactgagtct
    19921 tggtgcgacg ccgaagcagg cttcagcgat attgattcgc gacagtattc gcgcggcttt
    19981 aattccgacg gtcgattcag caaaaacggt tggcttagtg agtttaccag gaatgatgtc
    20041 cgggctgata tttgccggga ttgatccggt gaaggcgatt aaatatcaga ttatggtgac
    20101 ctttatgctg ctctcaaccg ccagcttgtc gaccattatt gcctgctatt taacctatcg
    20161 taagttttat aattcgcgcc accagttggt ggtgacgcaa ttgaagaaga aatgatgcga
    20221 tgccggatgc gcagcatcgc atccggcgtt gtggttcatg tgccgatcaa cggaatatca
    20281 atacaacaat gcatacagct ggcggcgata cttcgacgcc agtgcatcac ccgtacccag
    20341 cgcagcgagg atctcctgga acgttttacg tgtctgaccg tctgcggcgg tgagatcttt
    20401 acgcagatgc ccgaacagca actccagcgc ctcttcattg cgcccaacct ggtgcagttg
    20461 cagagccagt tgcgtcgcca gtgcggcatc ttctggattc tccgccacct gctgttgcaa
    20521 ctgttgaatt tccggcgtat cagccgcctg cttcagcagt tcgatttgcg ccaccagccc
    20581 ctggtagcgg gtgtcctgat cctgcaacgg aatggttttc agcaccgctt ccgcatcttc
    20641 agaacggttc agcgcaatca gcgtttctgc cagcagcagg ccgatctccc cgttctgatt
    20701 cgacaactgc caggcgtctt tcagcaatgg cagggcatcg gtgtaattgc cttcctgcat
    20761 cagttgcatc gcctgctgcg ctttcagctc ttcttcgcgc ggcagcactt tatccagcag
    20821 ggcgcggatc gcctcttccg gttgcggccc ctggaagcca tctaccggtt gcccgttctg
    20881 gaacagatac acggtcggaa tcgcgcgcag accaaactgg gcggcaatca tctgctccgc
    20941 gtcacagtcc agcttcgcca gaataaattg cccgttgtac tgcgccgcga ggctttccag
    21001 gatcggggtt aactgcaaac agtgctggct acgttcagac caaaaataga acagcaccgg
    21061 agtggtcatc gactgttcaa gaacctgttg caggttagat tcgttaatgt tgacaatatt
    21121 ttctacggac atggagtcgc tctctgttgt cgatattttc tttgacatgg gggcttaagc
    21181 gcgcgcttca actcacccct gcaatatttt gtccatcacg cgccccggca gcaggcgctt
    21241 gagcaccatt actgcccagg tcaccagcgt taccggatag cgcatcttcg gcttctcgct
    21301 aataaaagca tggcgtactt tgtccaccac cgcttccggt cccaacgtaa agcgggcggc
    21361 gatgccggga ttttcgactg gtttatcact ttgcgtctgg ttgacgttgt cggtgaagcg
    21421 agtacgaatg ggaccgggtt cgatcaggct gactttaatt ccgctgtggc gcagctccat
    21481 gcgcagtgcg tctgaccacg cctccagcgc atatttactg gccgcgtaag cgccacgacc
    21541 cggcgtggag attaatccca tcaccgatga tgtcatcaca atacgccctt caccgtgcgg
    21601 taacatcgcg ggtaacaggc gcatggtgag ctggtgtgcg ccgaaaaagt tggcggaaaa
    21661 ctgctgttcc atctgcgcac ggctgatggt ggaaaggggg ccatacatgc cgaatccggc
    21721 attgttaaag atcccataca gacaattatc ggtcagggcg attacctcgt ctgctgcgcg
    21781 atcaacactt tctggtgaat ccagatcgat caacacgccg gtaaatccca tgttgttcat
    21841 acgctcaaca tcatccggtt ttcggcaacc tgccagcaca tgaaaaccct ggcgttttaa
    21901 ttcgagcgcg ctttccaggc caattccact ggaacatccg gtaattaaga ccgatttttg
    21961 cataacttta cctgtcagga tctccgttgc tttatgagtc atgatttact aaaggctgca
    22021 actgcttcgc catccagtcg gcaataaacg gctgggcgtc gcggttggga tgaataccgt
    22081 catcctgcat ccattgtggc ttgaggtaga cctcttccat aaaaaagggc agcagcggaa
    22141 catcaaactc tttggcgagt ttggggtaaa tggcgctaaa ggcttcatta taacggcgac
    22201 catagtttgc aggcagacgt atttgcatta acaatggttc agcgttggcg gctttgacat
    22261 cctgcaaaat ctggcgcagc gtttgctcgg tttgctgtgg ctgaaaacca cgcaaaccgt
    22321 cattgccgcc cagttcaacc agcacccaac gcggctgatg ctgtttcagc agagccggaa
    22381 ggcgcgccag tccttgttgc gaggtgtcgc cgctgatgct ggcattaact accgacgttt
    22441 tactctgcca cttatcattc aacaaggcag gccaggccgc gctggcagac attcgatacc
    22501 cggcgctcag gctatcaccc agaatcaata acgtgtccgc tgcggcggca cggaaggtta
    22561 acaggaccag gaacaggaag ggcaaatgcc agcggaaaac attgttgaag ttcatcatct
    22621 taagaagtcc gtcggtcagg gggagcatga actctccatc ctcaccggag ttgagctggt
    22681 tgtcaaacgt ggcgagacca tcgcactggt gggcgagtcg ggatcgggta agtcaacctt
    22741 gctggcgatc ctcgccgggc ttgatgacgg cagcagtggc gaagtgagtc tggtgggaca
    22801 accgctacat aatatggacg aagaagcgcg ggcaaagttg cgcgcgaagc acgtcggctt
    22861 tgtttttcag tcatttatgt taattcctac ccttaacgcg ctggaaaacg tcgagcttcc
    22921 ggctctgctg cgcggtgaga gtagcgcgga aagtcgtaac ggggcgaaag cgttgctcga
    22981 acagttaggg ctgggtaaac gtctgggtca tcttccggca cagctttccg gcggtgaaca
    23041 gcaacgagtg gcgctggcac gagcctttaa tggtcgacct gatgtgctgt ttgccgacga
    23101 acccaccggc aaccttgacc gccagacggg cgataaaatt gccgacctgc tgttttccct
    23161 caaccgtgaa catggcacca cgttgattat ggtgacccac gacctgcaac tggcggcacg
    23221 ctgcgaccgc tgcttacggc tggtgaacgg gcagttgcag gaggaagcat gattgcacgt
    23281 tggttctggc gcgaatggcg ttcgccgtcg ctattaattg tctggctggc gctaagcctg
    23341 gcggtggcct gcgtgctggc gctgggcaat atcagcgatc gcatggagaa gggcttaagc
    23401 caacaaagcc gggagtttat ggcgggcgat cgggcgttgc gcagttcgcg cgaagtgccg
    23461 caagcatggc tggaggaagc gcaaaagcgc ggcctgaaag tcggcaagca gctgactttc
    23521 gccaccatga catttgcagg cgacacaccg cagctggcga acgtcaaagc ggtggatgat
    23581 atctacccga tgtatggcga tctgcaaact aatccccctg gcctgaaacc gcaggcgggc
    23641 agcgtattgc tggccccacg cctgatggcg ctgcttaacc tgaaaacggg cgacaccatt
    23701 gacgtgggcg atgccacctt gcggattgcc ggagaagtga ttcaggaacc ggattccggt
    23761 tttaacccct tccagatggc tccgcgtctg atgatgaatc tggcggatgt cgataaaacc
    23821 ggagccgtgc agccggggag tcgggtcacc tggcgctata aattcggcgg cagtgagaac
    23881 cagctcgacg gctatgagaa atggttgtta cctcagctta aacccgaaca acgctggtac
    23941 ggtctggaac aggacgaagg cgcgctgggg cgatcgatgg aacgctcgca acagttcctg
    24001 ctgctttcgg cgcttctgac cttgctgctg gcagtggcag cagtggcggt agcgatgaat
    24061 cattactgtc gcagtcgcta cgatctggtg gcgatcctca aaacgctggg ggcagggcga
    24121 gcgcaactgc gtaagctaat cgtcggtcag tggttgatgg tgctgttgct ttcggccgtt
    24181 accggtgggg cgataggcct gttgttcgaa aacgtgttga tggtgctgct caagcccgtt
    24241 ctgcctgctg cactaccgcc agccagcctc tggccgtggc tgtgggcgct cggcaccatg
    24301 acagtcatct cgctgctggt ggggctacga ccgtaccgct tgttgctggc aacgcagcct
    24361 ttacgcgtat tacgtaatga tgtggtagcg aacgtctggc cgctgaagtt ttatctgccg
    24421 attgtcagtg tggtggttgt gctgctgctc gccggattaa tgggcggcag tatgctgctc
    24481 tgggcggtgc tggcgggcgc ggtagtactg gctttgctgt gcggtgttct aggctggatg
    24541 ctgctgaatg tacttcgccg catgacgctg aaatcgctgc ctctgcgcct ggcggttagc
    24601 cgcctgttac gtcagccgtg gtcaacgtta agccagcttt cggcattttc gctctccttt
    24661 atgctgttgg cactgctgct ggtgttgcgt ggtgatctgc tcgaccgctg gcagcagcag
    24721 ctacctccag aaagcccgaa ctacttttta attaacatcg ccacagaaca ggtagcaccg
    24781 ctaaaagcgt tcctcgcgga acatcagata gtcccggaat cgttttatcc ggtggtgcgg
    24841 gcgcggctga cggcgattaa cgataagccg acagaaggta atgaagatga ggcgcttaac
    24901 cgcgaactca atcttacctg gcaaaatacg cggcccgatc ataatccgat tgtcgccggt
    24961 aactggccgc caaaagccga tgaagtgtcg atggaagagg ggctggcgaa acgcttaaac
    25021 gttgccctcg gtgataccgt gacttttatg ggcgataccc aggagttccg cgctaaagtg
    25081 accagcctgc gcaaagtgga ctgggaaagt ctgcggccta atttctattt tattttccct
    25141 gaaggggcat tagacgggca accgcagagc tggcttacca gtttccgctg ggagaatggc
    25201 aacggcatgt tgacacaact caaccgccag ttcccgacca ttagtctgtt agatattggc
    25261 gcgattttaa aacaggtcgg tcaggtgctg gagcaggtaa gtcgggcgct ggaagtgatg
    25321 gtggtactgg tcaccgcctg cggtatgttg ctgttgctgg ctcaggtgca ggtgggaatg
    25381 cgtcagcgtc atctggagct ggtggtgtgg cgcacactcg gtgcggggaa aaagctgctg
    25441 cgcacgacat tgtggtgtga gttcgccatg cttgggtttg tttctggcct ggtagccgca
    25501 attggtgcgg aaacggcgct ggcagtgttg caggcgaaag tgtttgattt tccgtgggag
    25561 ccagactggc gattgtggat tgttctgccg tgcagcggtg cgctgctgct gtcgcttttc
    25621 ggcggctggc tgggtgcgcg actggttaag ggtaaggcgc tgttcaggca gtttgcgggg
    25681 tgaagagagt aaataagggg cgttctggtt gaatggaacg cctagtttaa
//
LOCUS       DAZFXT010000064        25351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.064, whole genome shotgun sequence.
ACCESSION   DAZFXT010000064 DAZFXT010000000
VERSION     DAZFXT010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.064"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            90..221
                     /locus_tag="ACHKW0_002817"
                     /pseudo
     CDS             90..221
                     /locus_tag="ACHKW0_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutE"
     gene            complement(536..688)
                     /locus_tag="ACHKW0_002818"
     CDS             complement(536..688)
                     /locus_tag="ACHKW0_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7475621.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRVEQGNTVLVATL
                     AYEVKR"
     gene            1020..1262
                     /locus_tag="ACHKW0_002819"
     CDS             1020..1262
                     /locus_tag="ACHKW0_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001317518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475622.1"
                     /translation="MSNEYQSTSINPDVFEQFLTLLNTHCKSSETALAMLSTLCYELM
                     RRSGIHSLDSLMLNGEGILIQLQGADIREKTGQHIH"
     gene            complement(1355..2890)
                     /locus_tag="ACHKW0_002820"
     CDS             complement(1355..2890)
                     /locus_tag="ACHKW0_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7475623.1"
                     /translation="MSCIKEKSKYAFERAKQFYFMLWCKESLIYLLFSVLVTWCCGYT
                     VKNTLGNVLILFLMLLLGRQNVISATCVCIFLLLSACYAPVGMIYGKINNSFIASVMQ
                     TNINEAGEFITGIPVLYFIVSLVIAAFTFWFWRKRDKSHRGFIFLLFFIVLSVNAWPK
                     RMVTNVMTGIAETREEMMRYESLKNNQKDSWDIVSVEKKYKTIIIVIGESVRRDYLSV
                     YGYPLPTTPWLNSAPGIFINGYFSAAPNTIGSLSRTLTLDYTETGNPGNNIVTLARKA
                     GYETWWISNQGSLGQHDTLISVIAANADKKHFLKEGSSSSLRIDDEDMLQYVREAVNS
                     SSPKVIFIHMLGSHTNPCDRLFNDAQPFRELFGNKVSCYLSTLLKLDRFLQAIYNMTT
                     EHGDDFAMLYFSDHGQSVSSDSTPVHHAPGLRSGYDVPLMMMASDITEHHLNHVHISA
                     RRFPEIFQWITGIQTKNLPLSFSLLQNDFAESVSVFNGERDVLVMSLPEEPILTGNAE
                     SEK"
     gene            complement(3090..3545)
                     /locus_tag="ACHKW0_002821"
     CDS             complement(3090..3545)
                     /locus_tag="ACHKW0_002821"
                     /inference="COORDINATES: protein motif:HMM:NF022214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HFO7475624.1"
                     /translation="MRDKSEVLTGAQKNRFGLILYIILLSVVYILITRLNLTTTYYSL
                     YSSLGENSQVTEYFIISIATFLFLSGLCLLFIYSFFKRKRVVGKLLLAIHLLYISHYA
                     ITFVYCLYVVGAECMTTGKIEYILIDIIIAMLSLSYVCLSGRARHIFVN"
     gene            complement(3633..4256)
                     /locus_tag="ACHKW0_002822"
     CDS             complement(3633..4256)
                     /locus_tag="ACHKW0_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7475625.1"
                     /translation="MQERIDYHIEKYQFRARNESPRLMRQWADVMRECRETGAGARER
                     LRTALLNVDYVTSFELPFRLLLTRTPQMIDTLRREWLLSQKKALFNGRQRGCVYSLQN
                     DLSGVPDCFSYSLATRIRRMDCYGMTTRCFTDIAGQVKVPTARLKLALESGLLVTALD
                     GLFWLGTQRIAADVQRLRQSGMVIVTREVEVSDSLTGTTRLVSAYTL"
     gene            4415..4918
                     /locus_tag="ACHKW0_002823"
     CDS             4415..4918
                     /locus_tag="ACHKW0_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO7475626.1"
                     /translation="MIKYIFALTLLLSGSVCAVQIQGLVIRVLDGDTLEILQARHPVR
                     IRLVNIDAPEKKQPFGRWSMNQLKSLVANTPVTVTYTQTDRYGRILGRVYTEDGMEVN
                     RWLVQHGAAWVYPDFNTDYTLPAYQREARTMKRGLWADKKPVPPWLWRKENKNDHTEL
                     QINNILP"
     gene            5283..9374
                     /locus_tag="ACHKW0_002824"
                     /pseudo
     CDS             5283..9374
                     /locus_tag="ACHKW0_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001653304.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S6 family peptidase"
     gene            complement(9537..9746)
                     /locus_tag="ACHKW0_002825"
     CDS             complement(9537..9746)
                     /locus_tag="ACHKW0_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475627.1"
                     /translation="MKTNAPRLFFVDKESGVSFDVFHVLSVSFPVTAVPHMTGECNLR
                     YLTENVPFAGTGGQSCMQDLYHSSG"
     gene            9880..10242
                     /locus_tag="ACHKW0_002826"
     CDS             9880..10242
                     /locus_tag="ACHKW0_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475628.1"
                     /translation="MNDGRQTPPPPEAWKIRFLANGTPGTWFYTDDAAVTKEWLRAHC
                     DDVEVIPLCRMHDGPVFREHHINELSAALARMLHMHHILLERTRYRENIHDAECIREI
                     HEAPLQAIGILEKYKRTK"
     gene            10255..10446
                     /locus_tag="ACHKW0_002827"
     CDS             10255..10446
                     /locus_tag="ACHKW0_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000264906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475629.1"
                     /translation="MWSLIFWLSSPFNVSSGDARQPTLAVHSQAFRTEKACRDAFTAL
                     KKENVGEFALRGVCVPAGE"
     gene            10474..10785
                     /locus_tag="ACHKW0_002828"
     CDS             10474..10785
                     /locus_tag="ACHKW0_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlgM"
                     /protein_id="HFO7475630.1"
                     /translation="MTSPDLIQADLHRMSWSQLAKAAEESTVHHDYARALILWRHAYH
                     AATLTINKNLATAKINFCAKRILMRNQMSKIIRHTDTDERLFRLSKHHHLYEKKKTKE
                     G"
     gene            complement(10928..11023)
                     /gene="dqlB"
                     /locus_tag="ACHKW0_002829"
     CDS             complement(10928..11023)
                     /gene="dqlB"
                     /locus_tag="ACHKW0_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinQ-like type I toxin DqlB"
                     /protein_id="HFO7475631.1"
                     /translation="MINLLIIVLRAVVAVVNAVIALLELIRELID"
     gene            11522..12820
                     /gene="trbA"
                     /locus_tag="ACHKW0_002830"
     CDS             11522..12820
                     /gene="trbA"
                     /locus_tag="ACHKW0_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HFO7475632.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHIFSALSPAIAPVLADGDNNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVASTRQCFMAQLGQPLTSWKDMAPHEKALFAIFGLQ
                     FFLGDRKAAVALMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQKWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEACRLGLPCPEPCVEEAIEGLRQDMLRLGLIWDEPQPDRDRRRQIRTRWSLTDDVI
                     PRRHDNDEGSDTGETTETTETTETTETTETRHPADKEKAQ"
     gene            12817..13935
                     /locus_tag="ACHKW0_002831"
     CDS             12817..13935
                     /locus_tag="ACHKW0_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HFO7475633.1"
                     /translation="MTIRYTATLNNKHLSLTALYPDGGQEVIAILPVTELGALINRNN
                     RLMHLSFSTPFREITETLLVDTVDTERPGHHIGRMLSAIDSCLSAAYAELPARKTSFM
                     RGLIYGAGLVLALLTAVTAGYILLKPDSDSPLRVPVTESVTETAPLHIDKEIVRSVAP
                     SETKNEIVHPAPQEMQETSATPAITADKKTLVAEARRNLATVLKRNADRGMFTINLST
                     GHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGEASTNRTAPLLCEPD
                     AQKRATGWHQLYSADGGMAPTGDAPAAPDEKCLTAARAAIDVNNQAFRQFGFGGTPWV
                     LSDTGWHLPSGILKDATTVSLFLKATDTEDNHNEPASR"
     gene            13916..16219
                     /gene="trbC"
                     /locus_tag="ACHKW0_002832"
     CDS             13916..16219
                     /gene="trbC"
                     /locus_tag="ACHKW0_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugative transfer protein TrbC"
                     /protein_id="HFO7475634.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNIIGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFTLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETSPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LSWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVRERK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMILARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMMAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRRLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            16698..16928
                     /locus_tag="ACHKW0_002833"
     CDS             16698..16928
                     /locus_tag="ACHKW0_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475635.1"
                     /translation="MKTNMGSTKNGARVPHVLNRFTSMDIDMNSQILPDMLINFALIN
                     ITDRKNEGTNTIDGNWQADEGRRYRDNVRIYF"
     gene            complement(17026..17457)
                     /locus_tag="ACHKW0_002834"
     CDS             complement(17026..17457)
                     /locus_tag="ACHKW0_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0019534 - toxin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0043213 - bacteriocin transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity TolA C-terminal
                     domain-containing protein"
                     /protein_id="HFO7475636.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(17525..20236)
                     /locus_tag="ACHKW0_002835"
     CDS             complement(17525..20236)
                     /locus_tag="ACHKW0_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HFO7475637.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRMRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVEPGFRRDPVLRRERREARAAAREDLRARYLVW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPGMGFEPEGSYQQFNDVFAEMVAWHNAAGITGNGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(20248..20580)
                     /gene="mobA"
                     /locus_tag="ACHKW0_002836"
     CDS             complement(20248..20580)
                     /gene="mobA"
                     /locus_tag="ACHKW0_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization protein MobA"
                     /protein_id="HFO7475638.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            20813..21148
                     /locus_tag="ACHKW0_002837"
     CDS             20813..21148
                     /locus_tag="ACHKW0_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HFO7475639.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     VLVRTKGYTLADVRDTLKNAGYEVGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     NDGIDMNNN"
     gene            complement(21232..22080)
                     /locus_tag="ACHKW0_002838"
     CDS             complement(21232..22080)
                     /locus_tag="ACHKW0_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7475640.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARRILLDASGDIFLYGFEDCVTDSVRCMDKPEEAKKNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVWRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLIRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGELFEGEYFTVRGYQKGTVHIVFKRPDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            22262..22549
                     /locus_tag="ACHKW0_002839"
     CDS             22262..22549
                     /locus_tag="ACHKW0_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001677520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475641.1"
                     /translation="MAKNFVAGASHPGQMEREGVTSAPRKVHSGNRPQRWRQEGRLLR
                     HQAQFPHTPSGVQGAQVTARLKKNDGTTMACRGHWRTTPAQVKTEKTARHL"
     gene            22658..22909
                     /locus_tag="ACHKW0_002840"
     CDS             22658..22909
                     /locus_tag="ACHKW0_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7475642.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAEDV"
     gene            complement(22940..23161)
                     /locus_tag="ACHKW0_002841"
     CDS             complement(22940..23161)
                     /locus_tag="ACHKW0_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475643.1"
                     /translation="MRTAKPGKIPGFFASRSRASSMTIAERLIQKGALEVEREIAWRL
                     RDMGWTPERIQEATGLSGEELKKLFPDEQ"
     gene            complement(23161..24051)
                     /locus_tag="ACHKW0_002842"
     CDS             complement(23161..24051)
                     /locus_tag="ACHKW0_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HFO7475644.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLGCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGALEKALAIACQLQKMGMT
                     PEQIKQATGLSDDELKKITH"
     gene            complement(24116..24382)
                     /locus_tag="ACHKW0_002843"
     CDS             complement(24116..24382)
                     /locus_tag="ACHKW0_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475645.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(24475..24909)
                     /locus_tag="ACHKW0_002844"
     CDS             complement(24475..24909)
                     /locus_tag="ACHKW0_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HFO7475646.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
BASE COUNT         6816 a         6517 c         5858 g         6160 t
ORIGIN      
        1 tttataaaat taacaaagat actcatttag atgtataaac tataaacacc gtataataaa
       61 ataattcttg ctaaacaaga aattaaaatc ttgcatccac agtctcgcac accatgaaga
      121 cactcggata tgagtacaaa gcacgtttcc cgcagatgat ccggtatcgg gttaaagagt
      181 gggaaaaagt tgccggtgtg cctgttgcag aagtatttta aggtagcgac caactgttag
      241 acggtaatcc gtcagccggt tttctctgaa ccggcttttt cttttcaggc aaaatgcctg
      301 tctgccgtcc ctgtatcaga ctgtaattag cacaatacgg caatttgccg ttcagtatat
      361 tctaggaggt acaaatcatg ccatctgcaa cacaaactgc ccgactggaa gcaaggagca
      421 gcgcagaact gcatgctgtg ctgaaacgcg ctgcagaaat ccaggtaaaa aagggcgcat
      481 tgaggtttgc tttcacaaca atccggcggc agatttcctg ccgccgtgtt gcccgttaac
      541 gtttaacttc gtaggctaac gttgccacaa gcactgtgtt tccctgctcg acacggaatc
      601 cgcaaagcga atccctcacc atccagacaa aacacagtat cgtcacacag acgacgatta
      661 acatcattaa aaacgttcgt tgtggcatcg cagcccctcc attgcctttc ggccgtcatc
      721 gggctactat aatgttgtct aggcattaca gtgggccctg ggttgattga taaaaaatcg
      781 cctggggctt tctgctacat gccgtccaca aaacgctttc ggcatacagc ctcaagcgcc
      841 cgccgctatt ctaatcgatt ttcagtctgc cacaatcgtt ttccacctaa tgacatgtta
      901 tgcacaacag tatttttacg ggaaactaca cggcaaggat tcccggaaca cgttcattat
      961 ttgatggtta cagacatcat gttaaatccg gaagtaataa tgacctgaaa ggaaatacta
     1021 tgagtaacga atatcaaagc accagtataa accctgatgt ttttgaacaa tttcttaccc
     1081 tcctgaatac acactgtaaa tcatcagaaa ctgcacttgc catgctgagt acactctgct
     1141 acgaactgat gaggaggagt gggatccatt cactggacag cctgatgctc aacggagaag
     1201 gtatattgat acaattacag ggggctgata ttcgcgaaaa aacaggtcag catattcact
     1261 gatccaggga tgtgctgaaa acagtgtcag cctgaaataa acgttcaatg cctcatacac
     1321 atgaggcatt gagcacggtc ataaataatc tttattattt ttctgattca gcatttccgg
     1381 tcagtatcgg ctcttctggc aaagacatta ccagaacgtc acgttcacca ttgaatacag
     1441 aaacactttc agcgaaatca ttctgtaaca aagaaaaact taacggcaga tttttggttt
     1501 gtatccctgt gatccattga aaaatctcgg gaaaacgacg tgcactgata tgcacatgat
     1561 tgaggtggtg ctcagtaata tcactggcca tcatcatgag aggtacatcg tatcccgaac
     1621 gcaaacctgg cgcatgatgc acaggggtgc tgtctgaact gacggattgc ccatgatcag
     1681 aaaaatacag catggcaaaa tcatcaccat gctctgtcgt catgttataa attgcctgaa
     1741 gaaaccgatc cagcttaagc aatgttgata aataacagga tactttattt ccgaataatt
     1801 ccctgaatgg ctgtgcatca ttaaaaagtc ggtcacaggg attagtgtga gaccccagca
     1861 tatgaataaa aatcactttt ggagaagacg agttaacagc ttccctgaca tactgaagca
     1921 tatcctcatc atcaatgcga agggaagaag agctcccttc cttaagaaaa tgttttttat
     1981 ccgcattggc ggcgatgaca gaaatcagcg tatcatgttg ccctaaagag ccctgatttg
     2041 atatccacca cgtctcatac ccggcttttc tggcaagcgt aacaatatta ttacccggat
     2101 tccctgtttc agtgtaatca agcgtcagtg ttctgctgag tgaacctatc gtattcggcg
     2161 ctgctgagaa atacccatta ataaatatac ccggagcaga attcagccat ggtgttgtcg
     2221 gcaaaggata gccataaaca gaaagataat cccgtctgac actttcacca attacgataa
     2281 ttatggtttt atatttcttc tccacactca caatatccca tgagtctttc tgattgtttt
     2341 tcagcgattc atatcgcatc atttcttctc tggtttcagc tatccccgtc attacattcg
     2401 taaccatacg ttttggccat gcatttactg acaatacgat aaagaacaac agaaagataa
     2461 atccccggtg gcttttatca cgcttacgcc agaaccagaa tgtaaaagca gcaatgacca
     2521 gcgaaacaat aaaataaaga acaggtatgc ctgtgataaa ctcacctgcc tcatttatat
     2581 ttgtctgcat tacagacgca ataaaactgt tgtttatttt cccgtaaatc atcccaaccg
     2641 gagcatagca tgcacttaac aaaagaaaga tacagacaca cgttgctgaa ataacattct
     2701 gccgccccaa caacaacatc aggaacaata taagcacatt ccccagggtg tttttcactg
     2761 tatatccgca gcaccatgta acaagcacag aaaacaacag ataaatcagt gactctttgc
     2821 accacaacat aaaataaaac tgttttgctc tctcaaaagc atattttgat ttttctttaa
     2881 tacaagacac gccagtcatt ttcctgtaca aacacagaca atcatttcaa cgcatggata
     2941 tttatatgaa acagctcaca aaaacatcct gttctgtcca gcaagattga catattatca
     3001 ggttctgtgt ttacagcaac tgctccccga accagattat tcgcataacc cgatgaaggc
     3061 ctcataacca taaaacactc agaatacatt tagttaacaa aaatatgtct tgccctcccg
     3121 gaaaggcaaa catacgaaag agaaagcatg gctataataa tatcaatcaa aatatactca
     3181 atctttccgg ttgtcataca ctcagcaccg acaacataaa gacaataaac aaaagtgata
     3241 gcatagtgcg atatataaag gagatgaata gccagaagta attttcctac cactcttttt
     3301 cgtttaaaga aagaataaat gaatagcaaa cataaccctg acagaaaaag aaaggtagca
     3361 atggagatta taaaatactc cgtcacctgt gaattctcac ccagagatga atacagcgaa
     3421 taatatgtgg ttgtgagatt aagtcttgtt atcaatatat aaacaactga taatagtata
     3481 atataaagaa tcagaccaaa tctgtttttc tgcgctccgg ttaatacttc tgatttatct
     3541 cgcataaaat atcctgtctg taatctgtcc cggtaaacaa tggtaaattt cagaatcagt
     3601 gcgaatacgg taacaaagat tcctcctgtg ctttaaagtg tgtaggcaga aaccagtctg
     3661 gtggttccgg tcagggagtc tgatacctca acttcccggg tgactatcac catacctgac
     3721 tgacgcagtc tctgcacatc agcggcaatt cgctgagttc ccagccagaa caggccatcc
     3781 agtgccgtta ccagcaaccc gctttccagc gccagtttca gccgtgccgt cgggacttta
     3841 acctgcccgg cgatatcagt aaaacaacgc gtcgtcatgc catagcagtc catccgccgt
     3901 attcgcgtcg caagactgta gctgaaacaa tccggcacgc cggataagtc attctgcaga
     3961 ctgtatacac atccccgctg ccgcccgttg aacagggctt ttttctgact gagcaaccat
     4021 tcccgacgca gagtgtcaat catctgcggt gtccgggtta acagcagacg aaacggcagc
     4081 tcaaaactgg tcacataatc aacattcagt aatgctgtcc gcagtcgctc cctggcacct
     4141 gccccggtct cccggcattc acgcatgaca tcagcccact gacgcatcag tcgtggagac
     4201 tcattcctgg ccctgaactg atatttctca atatgataat caatacgttc ctgcatatcg
     4261 ccaccagata tctgttgttt acccagacac agtgattagc ctacaggaat aaatatcctc
     4321 cgtgaagcca ggtgcctcag aagataaaaa catgtatttt ttcgacgcgt aatattaacg
     4381 tcatccccat aaaaataaat caggaaaata taaaatgata aaatatatat tcgcactcac
     4441 tcttctgctt tccggttctg tctgtgccgt tcaaattcag ggactcgtta ttcgtgtgct
     4501 ggacggtgat actctggaga tattacaggc ccggcacccc gtgaggattc gtcttgtgaa
     4561 tattgatgcg ccggaaaaga aacagccttt tggacgctgg tcaatgaatc aacttaaaag
     4621 ccttgtggca aatacgcccg taaccgtgac atacacacag acagaccgct acgggcgtat
     4681 tctgggacgg gtatataccg aagacggcat ggaagttaac cgctggctgg tacagcatgg
     4741 tgcagcctgg gtatatcctg atttcaacac agattacacc ctgccggcat accagagaga
     4801 ggccagaaca atgaagcggg gtctgtgggc agataaaaaa cctgttcctc catggctgtg
     4861 gcgaaaagaa aataaaaatg accacacaga attacaaata aacaacatct taccgtaata
     4921 tgtatttttt ctgctgcatt atcttccctg tttacagtca gcaaaaaaac gtccgaagca
     4981 aaacaaacac gaataataaa cctgcccgat gcatccatta caaaaatgaa tccttcctct
     5041 tatggaaaat acagtaacca caatgaaaaa taaacagata aagatgatac ttatctcaca
     5101 aggaaaagcc ccggcaaaaa cataagattc acttaacttt ttcaccttgt agaatcttct
     5161 tttttcatgg acatgttatt ttgttgattt taaaatatca aaatgggtga atgtatcaca
     5221 tcactcataa agtggatatt atcacgaaac tatctattaa acagcagatt agcagataca
     5281 acatgaataa aatttactca ctaaaatata gtagccttac tggtgggctt atagctgtat
     5341 cagagttaag taaaaaagtt aaaggaaaaa caggcagaaa attaatgact gcttccgttg
     5401 ctttatctgt gagtctttca gccttacccg cagaggcatc aacggtcagc gctgaaatac
     5461 catatcagac tttccgtgat tttgctgaaa ataaaggcgt atttacacct ggtgcaaccg
     5521 gaattgaaat aaaagacaaa aatggaaatg cagttggtac gcttgatgtg ccaatgattg
     5581 acttcagcag cgtgtctcgg cggggctcac tgaccctgtt gtcgcaggga tatggtgtat
     5641 cagcaaaaca cggaggcctt ggtgatgtaa acaatgccag ttttggctat gataaaaata
     5701 attatactgt agtaaagaac aacaaacatt cgggccttga tttttctctg catcggttca
     5761 gcaaactcat cacagaggct gcacctgctg atataaatat atccggacaa cttagtgatt
     5821 cgtcacagta tactgctttt tacagagcag gcgctggcac acagtatatt aaagaacgct
     5881 caggtaaaca gactcatatc ccgggaacat ttttaactgg tggaacagta ggcaccccat
     5941 agtattcagg aaataattta atatcaagct ctccaggtga cacctataat aaatcacagg
     6001 gaccattagc cagttatggg cagatgggtg acagcgggtc gccattattt gcttatgatt
     6061 ctctcagtga aaaatggtct ctggctggag tcaccctgca taataatggt gttaatggcc
     6121 aaaaaaacaa ctggttgtta ttacctgaag attatattaa aaatattatt acagccgatt
     6181 tcgatcccat tatttcattt aataaaaaca gtaaagaaca tatgtcatgg acatatgatg
     6241 cagccaaagg cgtgggccgt attcagcagg atgaccaaca gtttgtgatg catggtaacc
     6301 tgaatggtaa tctgaatgca ggtaaaaatc tttattttac aggcgaaaat ggcattattg
     6361 atttaaaaga taatgttaat cagggggccg gatatcttca gtttgctgat gattatacag
     6421 taacaacgag caatgacagt tcatggtccg gaggaggcat tattgtcaat tatggtacaa
     6481 ctgtaaaatg gggtataaac ggggtttccg gagatgacct tcataaagtt ggggatggaa
     6541 cgcttattat taatggcaca gggaaaaatg aagggggact gaagattggg gctggtactg
     6601 taatccttga acaaaaagcg aaaaacaacg attcaacggc tttttcttcc attaacatca
     6661 gtgggggaaa ctcgcgtgtt aaactctcag gagataatca gattatacct gataatgttt
     6721 cctggggatt ccggggtgga tatttagata taaacggaaa aaacacagag ttctcccgct
     6781 tacaggctgt tgattatggg gctgcaatta ttaacagcag cacagacaag tcattattga
     6841 ctttaaatct gtcaccattg aaaaaagatg aaatagctgt aagcgtgaag gcgttagata
     6901 tgaacgccat ctttcagggg ggacacggga cagccggaga tttgtataaa acaacttttt
     6961 acggtccgac acagtattat ctgctgaaaa agcccaaatt tggcagtgtg ctgatggggt
     7021 cactgaaaaa caccagcgaa tggcaatttg caggaacaga cctgaatcag gccgttgata
     7081 tggcaaagaa taataaactg acatcaagtg cacaggcttc atatctttat cacggtaagc
     7141 tcctggggaa tatggatatt gtcattccgg agcttacagg caatgatatt ttaacgcttg
     7201 atggttctgt cagtatatca ggagatatgt caaaacagga cggtgctctt atcttccagg
     7261 gacacccggt tattcatgca gggcaaactg tttctgcatc gcagagtgac tgggagaaca
     7321 gggagttctc actcaacaat ctgaatctta ataatgcgga cttcagtctg tcccgtaatg
     7381 catttatgaa cgggaatatc agggccgtta accagagcac tgttattatc ggcggagata
     7441 cagtctttac tgataaaaat gacggaacag gtaatgatgt catcagtgtt gaagggaaat
     7501 ctgctgccgc aggaacatcc tcctatacag ggcatatcac tctggagcaa aaatcagcac
     7561 tggatatccg cgataatttt cgtggcgggg ttacgtctga agacagtcat atcaatgttt
     7621 cttcatcttc agtcctgttc tcagatgcat cgtcatttat aaacagctcc ctgaatattc
     7681 ataaaggagg tgcgctgacc gctcagggag ggctgtttac aagtggaagc attgatattg
     7741 gtgacgcttc ccttctgctt accggtacac cagtgaattc agatgatgct gcttttttac
     7801 cgaccatcaa tatggctgat ggcggattta aactgatgtc tgattcatca gtactgaaag
     7861 ccagagacca ggcatctgtt gttggtgata ttatttctga taaacaggcc acaatcagct
     7921 tcggaactga atcaggtaaa gagggcatat tgtctgagaa ggcatcccgg ggactcgcgg
     7981 taggattact gagtggtttt aatacggcat accgcggtgc aattcatgcc ccgtctgcat
     8041 ctgccactgt gaacaatacc tggtggcaac tgacaggaga ctcctcactt cgctcgttaa
     8101 aaaataccgg aagcatgaca tattttacag gaagtgcagc gaataaagca ttccatacac
     8161 tgacggttga tgagctgacg acgaatggca ctgcgtatgc catgcgtacg gacctgaaaa
     8221 atgcggataa gctggtagta aacaaaaagc tgtcaggtaa ggacaatatt ctgttggttg
     8281 attttctgaa caaaccctcc ggagaaaaac tggatattga actggtgagt gcaccgggga
     8341 acagcagtaa agatgtcttt aaaggaagtg aacaggcgat aggtttcagt aatgtaacgc
     8401 cggtcattac aacccgggaa accgatgaca aaataacatg gtcactgaca ggctataaca
     8461 cggttgcaaa caaggaagca acccggaatg ccgccgccct gttctctgtt gactataaag
     8521 cgtttctgaa cgaggtcaac aacctgaaca aacgtatggg ggacctgcgt gacatcaacg
     8581 gcgaagccgg tgcatgggca cgcatcatga gcggtaccgg ctctgccagt ggtggtttca
     8641 gtgacaacta cacgcacgtt caggtcgggg tcgacaaaaa acatgagctg gacggactgg
     8701 atttgtttac cggtttcact gtcacacaca ctgacagcag tgcctccgcc gatgttttca
     8761 gcggtaaaac gaagtctgtg ggggctggcc tgtatgcttc cgccatgttt gattccggtg
     8821 cctatatcga cctgattggc aagtatgttc accatgataa tgagtacact gcaacctttg
     8881 ccggactcgg aacccgtgat tacagcacgc attcatggta tgccggtgca gaagcgggct
     8941 accgctatca tgtcactgag gatgcctgga ttgagccaca ggctgagctg gtttacggtt
     9001 ctgtatccgg taaacagttt gcatggaagg accagggaat gcatctgtcc atgaaggaca
     9061 aggactacaa tccgctgatt ggccgaacgg gtgtggatgt gggtaaatcc ttctctggta
     9121 aggactggaa agtgacagcc cgtgccggtc tgggctacca gttcgacctg ctggctaacg
     9181 gcgaaaccgt attgcgggat gcatctggtg aaaaacgcat caaaggtgaa aaggacagcc
     9241 gtatgctgat gtccgttggc ctgaatgcag aaatcaggga taacgtccgc tttggactgg
     9301 agtttgagaa atccgccttt ggtaagtaca acgttgataa tgcagtcaac gctaacttcc
     9361 gttactcgtt ctgatactcc gtattcaaaa gggcctgcgg gccctttacg ctcagtgcct
     9421 gccgccctga tatgtctgag agacatttcc gggatgtctg tcgactggtt tacttaactg
     9481 cgatggttaa agctggcagt aatgccggct tttttacggt cgcacacgtc ttcctctcat
     9541 ccggaactgt gatacaaatc ctgcatacag gattgaccgc cggttcctgc aaatggcaca
     9601 ttttcagtaa gatagcgaag gttacattcc cccgtcatgt gtggcacagc tgtcaccggg
     9661 aaagaaacgg aaagcacatg aaaaacatca aaactcacac cggactcctt atccacaaag
     9721 aacaaacgcg gcgcgttcgt cttcatgaaa cacccactgc ctggtgccac acgcaccggg
     9781 aatgctacag caaaacgacc ggtcgccgct gcggcagccc tgacagtctc agcaggctga
     9841 ttctgagcag cattcagccc atagaatgag ataaccccta tgaatgatgg cagacagacg
     9901 cctccgcccc ccgaggcatg gaaaatccgg tttctggcga acggtacacc cggcacatgg
     9961 ttttacaccg atgacgcagc cgtcacaaaa gaatggctgc gggcacattg tgacgatgtg
    10021 gaagtgatcc ccctgtgccg tatgcatgac ggtcccgttt tccgggaaca ccatataaat
    10081 gaactcagcg ccgcactggc cagaatgctc catatgcacc acatactgct ggaacgaacc
    10141 cggtacagag aaaatatcca tgatgcagaa tgtattcgtg aaatccacga agcccccctg
    10201 caggctatcg gcatcctgga aaaatacaaa cgcacaaaat aataaggctg ttctatgtgg
    10261 agcctgattt tctggctctc atcccccttt aatgtctcct ccggcgatgc ccggcaaccc
    10321 acactggctg ttcacagtca ggcgttccgt actgaaaaag cctgtcggga tgccttcacc
    10381 gcgctgaaaa aagaaaacgt gggggaattt gctctccgtg gcgtctgcgt ccccgccgga
    10441 gaataaacaa gacatgcgca accggagtcc gctatgacat ccccggacct tatacaagct
    10501 gatctgcacc gaatgtcctg gtctcagctg gcaaaagccg cggaagagtc gacagtacat
    10561 catgattatg cccgggcgtt aatattgtgg cggcatgcat atcatgccgc cacgctgact
    10621 attaataaaa acctggcaac cgctaaaatc aacttctgtg ctaagcgcat tctgatgcgt
    10681 aatcaaatga gcaaaataat cagacataca gacacagacg aaagactctt ccgtctgagt
    10741 aaacaccatc atttatatga gaaaaagaaa acaaaagagg gatgacaatc ccatgatatg
    10801 ggaaaaactt gccatgctta ctgcaggtgc atttttgata ctggcagtcc tgtgggggta
    10861 ataatatcct gtttattaaa gggcagaagg cggtgcccct ctgcccttaa gtacgtttcc
    10921 gggattatca gtcaataagt tcccggatca gttccagaag agctatcact gcattgacca
    10981 cagcaacaac tgccctgaga acgatgatta acaggttaat catccgtttc tccgtcaatg
    11041 gaggaaatca ctgttgttta ctcttacatt gcctgtcaca acagctcttt cattgttaga
    11101 tgtaaagtga tttctctcca gccagagcac tgaacacagc accacctgtt cgatcagcca
    11161 ggacttaaaa tctcgcgttc atcgtcccat tggagatcac ggcagaagca tcaccagaca
    11221 gagctaacgg agcgattata gtcatttttc tgattgcaaa aaagagaagc gcagcctact
    11281 atgatggtgt tagatgtaga gtgatttccc tttcggtcag agtcaccaac tccgccccgg
    11341 aatcgcaaaa aaaccgacct caccagtcgg tttttttgtg cctgtctctc actgccttct
    11401 gctgttcctg tgaaaatacc ctggttattt catccctgcc cggtcattac cggcgattgt
    11461 tcgcattaat tccactcagc ctcatcccga aatttatcta tttatcgatg gaggaatccc
    11521 cgtgtcttac caacagacgt ctgccgccga agatccaatg gctatatggt atatcgtcgg
    11581 tgccatatgc ctgctctttg cgattatcat ctggcgcttt ttacctgaaa tcgtcttcgc
    11641 ctcctgcctg atcctgcaca cactgtgggg catgattgac tgggggccct ttcacaactt
    11701 tgccgccccc cgttacaacc tgctggccat cactgctaac aacgcggcaa ccatcacttt
    11761 cagccagtgg ctggatgtta tgtcccggac agtggggatc ctctggttga ttctgcttcc
    11821 catgacattc ggctttctgt ggatgtggtt tcaccatccg gcccagcccc gttttacccg
    11881 caggccactg aatatccaca cactgcccca tattttttca gctctgtcac cggcgatcgc
    11941 ccccgttctg gctgatggag acaacaacag actcttccac ggacaaaaac gtcccgagcg
    12001 ccgtgtcgca ctaacccccg aagcctttgt ggagcagaac aaccttatcc gcaacatgca
    12061 gctcgatgtt gcgtccacac gccagtgctt catggcgcaa ctggggcaac cactgaccag
    12121 ctggaaagac atggccccac atgaaaaagc gctctttgcc atctttggcc tgcagttctt
    12181 cctcggtgac cggaaagcag cggtagccct gatgaacaac ctcaatctct cctgcaggct
    12241 caaaagtaaa cgggaccagg ggcgtttcag cacgccggtg tattcactgg ccagaaacgc
    12301 ctttatccgg gtcatcaaaa cagaaggcgc acaaaagtgg ctcaggcagc accgttacgt
    12361 tcgcagcggt ctggtctggc tttacgcaca tgatttgcgc ctgacgcctc cgaactggct
    12421 gtggctcaag ggcgttgacc ggacgctctt ctatgccctt caccgggcaa acaccacaaa
    12481 aggcttcatc gaaggggccg gcgtggtggc cgttgcccgg gcagaaaatg aagcgtgtcg
    12541 tctcggcctg ccctgcccgg aaccctgtgt ggaagaagcc attgaagggc tgcggcagga
    12601 catgctgaga ctggggttga tatgggacga acctcagcca gaccgcgatc gcagacgtca
    12661 gataaggacc cgctggtcac tcacggatga cgtgatacca cgccgccatg ataacgatga
    12721 aggctcagat actggtgaaa ccaccgaaac cactgaaacc actgaaacca ctgaaaccac
    12781 tgaaacccgg catcccgccg acaaggaaaa agcacaatga ccatccgcta cactgcaaca
    12841 ctgaacaata aacacctgtc cctgaccgcc ctgtaccctg acggtggtca ggaggttatt
    12901 gccatcctgc cggttacaga actgggcgca ctgattaacc gtaataacag gctgatgcac
    12961 ctgtccttca gcaccccttt tcgtgaaata accgaaacac tgcttgttga taccgttgat
    13021 actgaacgtc ccggacatca catcggcaga atgctctccg cgattgacag ctgtctgagc
    13081 gcggcatatg cagaacttcc tgcccggaaa accagcttca tgagagggct gatatacggt
    13141 gcaggattag tgctggcact tctgacagcg gtaacagccg gatatatttt actgaaaccg
    13201 gacagcgatt cccctctcag ggtacctgtc acagaatccg taacagaaac agcaccatta
    13261 cacatcgaca aggaaattgt ccggtcagta gccccgtcag agacgaaaaa tgaaatcgtt
    13321 cacccggcac cacaggaaat gcaggagaca tccgctaccc cggcgataac agcagacaaa
    13381 aagactctgg tcgctgaagc ccgccgtaat ctggccactg tacttaaacg caacgcggac
    13441 cggggtatgt tcaccattaa tctctccact ggtcatgaac gcacactcta cgccttcctt
    13501 gaccccgcat gcatcaactg ccgtcttctg gagcccgcgc ttaagcaact ggcccgggaa
    13561 ttcagcgtgg tgatttatcc ggtctccgta atcggcggag aggccagcac aaaccggact
    13621 gcgccactgc tgtgtgaacc ggacgcacaa aaacgggcga ccggatggca tcagctgtat
    13681 tccgcagacg gtggtatggc acccaccggc gatgcaccgg ctgcaccgga tgaaaagtgt
    13741 ctgaccgctg ctcgtgctgc catcgatgtc aataaccagg ctttccgcca gttcggtttt
    13801 ggcggtaccc catgggtgtt atccgatacc ggctggcacc tgccgtccgg catcctgaaa
    13861 gacgccacta ccgtcagcct gttcctgaaa gccacagata ccgaggataa tcacaatgag
    13921 ccagcatcac gttaatcctg acctcataca ccgcacggcc tggggaaacc cgctgtggaa
    13981 tgcactgcac aacctgaaca tcattggtct gtgtctggcc ggcagtatca ttacggccct
    14041 gatatggcca ctggcccttc ctgtctgtct gctgttcacc ctggtcacct ccgtgatatt
    14101 cacgctgcaa cgctggcgat gccccctgcg tatgcccatg acgctcagcc ttgatgaccc
    14161 ctcccaggac agaaaggtca ggcgaagcct cttcagcttc tggcccaccc tgtttcagta
    14221 tgaagcagat gaaacatccc ccgcacgggg gattttttat gtcggttacc gccgtatcaa
    14281 tgatatcggt cgtgagctct ggctgagcat ggatgacctc acccggcatg taatgttttt
    14341 tgccaccacc ggtggcggta aaacagaaac cacctttgcc tggctgctca acccgctcag
    14401 ctggggaaga ggctttacct ttgttgacgg aaaggcacag aacgatacca cgaggacaat
    14461 ctggtatctc tcccgacgtt ttggccggga ggatgatatc gaggtcatca acttcatgaa
    14521 cggcggaaaa tcccgcagtg aaatcattca gagcggcgaa aaatcccgcc cacagtcaaa
    14581 cacctggaac ccgttcgcat tcagcacaga agccttcacc gccgaaacca tgcagtccat
    14641 gcttccccag aacgttcagg ggggagaatg gcagtcacgg gcgatagcca tgaacaaagc
    14701 cctggtcttt ggcacaaaat tctggtgcgt ccgggaaaga aaaaccatgt ccctgcagat
    14761 gttacgggag catatgacgc tggaaggtat ggcaaaactc tattgccgtg ggcttgatga
    14821 ccagtggccg gaagaagcca tcgcaccact gagaaattac ctgcaggacg ttccgggatt
    14881 cgatatgtca ctggtgagaa cgccgtcagc atggactgag gagccccgta aacagcatgc
    14941 ttatctctcc ggtcagttct cggaaacgtt cacaaccttt gccgaaacct tcggggacgt
    15001 ctttgcggca gatgccggcg acatcgatat tcgcgacagt attcacagcg accgcattct
    15061 gattgtgatg atccctgcac tggacacatc ggcccatacc acgtcggcac tcggtcgtat
    15121 gttcgtcacc cagcagagca tgatcctcgc ccgtgacctg ggctaccgcc tggaaggcac
    15181 agatgcacag accctggaag tcaaaaaata caaaggcagc ttcccctata tctgtatcct
    15241 tgacgaagtc ggtgcctact acacagaccg cattgctgtg gaggccaccc aggtacgttc
    15301 actggagttt tccctgataa tgatggcgca ggatcaggag cgaattgaag ggcagacctc
    15361 cgccaccaac accgccacac tgatgcagaa cgccggcacc aaattcgccg gacgcattgt
    15421 cagtgacgac aaaacggcaa gaacggtgaa aaatgccgcc ggtgaagagg ctcgggccag
    15481 aatgggcagc ctgcagcgcc acgatggtgt gatgggtgag tcctgggtgg acggtaacca
    15541 gatcaccatc cagatggaaa gcaaaatcga cgtgcaggat ttgatcagac tgaatgccgg
    15601 tgaattcttt accgttttcc agggggatgt ggtgccgtca gcctctgtgt atattccgga
    15661 cagcgagaaa agttgcgaca gtgatccggt ggtcatcaac cgctatattt ccgttgaagc
    15721 cccccggctg gagcgtctcc gccgtctcgt ccccaggaca gtgcagcgca ggctccccac
    15781 accggagcat gtcagcagca tcatcggtgt actgacagca aaaccatccc gtaaacgtcg
    15841 taaaaaccgt acagagcctt accgtatcat tgatacattc cagcgccggt tggcaacagc
    15901 gcagacatca ctggatctgc ttccgcaata tgatacagat atcgaatccc gggcaaacga
    15961 actgtggaaa aaggctgtgc acactatcaa taacacaacc agggaagaac gccgggtatg
    16021 ttatatcaca ctgaatcgtc ctgaagagga acactccggt cctgaagata taccttcagt
    16081 gaaggcgatt ctgaatactc tgctccccct ggagatgctg ttacctgtac cggatctaac
    16141 tgcatccccg ccccataaaa aaaatgtcgc tcagacacca tcaggtggaa aacaagagag
    16201 tcgtaaaaaa agattctgat aacacctgta tggctgattt taataacaac cttaataaca
    16261 acctttacgc gattattaat ctcctccgct caccatagta agcggaggat tcaggatttc
    16321 aatccatcat cagaggtaca ataaatgtgt attagtagcc cggaaacaaa ctcatggtct
    16381 gttatttatc gtaaaaattc cggagaagat atcaatatca cttccctgac gtttaaaaat
    16441 agtcttctgg cagcccggat actgatggtg cccaaaaact atatgatatg catactcaga
    16501 aacggggaga gggtcagacg atgggacaga gaaatacttg caggctcaaa cagatggtac
    16561 aagtgttctc ccgataattt cgaaatactg ggtaagctcc ccataataaa taaagtcaca
    16621 acactgataa aatcataatc tactgatttt actcagttaa gctaaatcag gatgaacgct
    16681 ttgattatga attatacatg aaaacaaata tgggcagcac aaaaaatggt gccagggttc
    16741 cccacgttct taatagattc acttctatgg atattgatat gaattcccag atcctgccag
    16801 acatgctgat taattttgcc cttattaaca tcacagacag aaagaacgag ggtaccaata
    16861 ccattgatgg taactggcag gccgatgaag gacgccgtta ccgggataat gtaagaatct
    16921 atttttgagt atgtcaacat aacctgaggc aaagtacctc aggttccagt gtagtaatca
    16981 gccaacctga cagtcttctg ttagccctac agatatgtcg tggagctaac cgcgcatgtc
    17041 aaagccggat ttttgaaaat ctctataaac ttccggattg ttgaaggccg ggaatttagc
    17101 tttatgggct gctgacttta tcgcttcgca ataggcttta tcgccattac tggttgatat
    17161 ttttaacgcc gtgccatccc gggagaattc catatgcaac ctgcattttt tccttttcca
    17221 tttttgcgga tcatcaagtt tagcattaat tgcatccctg attccccgtg cctgctcccc
    17281 ccattcatcc tgatcatccc aacgtcctga actgcaacta cccagggctg tagttttatg
    17341 gcaatttgaa ggatgtaatg gcgcacatcc cgccacaaag ctgactaaaa tagccagcat
    17401 aacgattttc tttaccccca tatattgctc ctcaatccat atagatctca gtaccatagc
    17461 tctgtcagag caaagaaaat cttaactaat acaagttaag atttttaata tccactcctc
    17521 atccctacat tccaggtgat ggtggcgtcc actcattatc ctgaactgga gcataagatg
    17581 cgccatgccc cccggatatg cttttctgtt gcctgatata ttcgtggtag tactgttcac
    17641 tacgctgacg atgtaaatcc acatccgggc ggctgattac aaagtgtcca tttccggtta
    17701 ttccagctgc attatgccag gccaccattt ccgcaaatac atcattaaac tgttgatatg
    17761 agccttccgg ttcaaaaccc attcctggct cacgatcaaa caatacagga gcaatcagat
    17821 tcagcttatc caccagttca tttctgtcct gatgatggta gaacaccaca cggtccccat
    17881 aatccacaca gactaactcg ccattcctcc gatcacgaaa acgcacactg ccatctttct
    17941 gcagacgggc ttcgagaacg ccagtgtctt cataaagtgg agttcctccc ggttcacaga
    18001 gaatcacgca gcggtcggca ttcgttgttc gtcgcttgtc tttgcgacga tcacgataat
    18061 cccagccacg taactgtgat aatgcagctc tgtctccctg taccgcctgt tgctccaccc
    18121 actgccggta ggacaaagga taccacttac cctccgcaat cagtgacaac cgctcttcct
    18181 tcacactctc cttcagcctg atcagcgcct gcatacgctg cacttccgca atatggtaat
    18241 gcagcttacg caattgtgga tcccggaact gcacacggat atacgccttg cgccgacggc
    18301 aggcagcatg aatttcccgt aaccgttctc cgtaacgtaa gtcgggttta cgccagtgct
    18361 ccttccatac aagataacgc gcgcgcaaat cttcccgcgc agctgcacgg gcttcacggc
    18421 gttcacgacg cagtacagga tcgcgccgga agccgggctc cacttcatcg ctggcagcaa
    18481 gttccggatt gtagcgacat tcaggcttca cccgttcaaa tatatctgca cctgcaatct
    18541 caaatggccc tgcctgcggc tcagccctgg ataacgtcaa atccgggtgg atactgctgg
    18601 ccttcactgg tgtgagctca tgattaaacg catccacaat cacaagacca tgatgctgtt
    18661 tctgcaacat aagcccttta cgcgcaaaca tctcatggca cgcctgccag ctgaaattcc
    18721 cgcccgcgat catatcttca atcaacgact cacactgaac agtcgtgtac ccccacagac
    18781 tttccttatc agaaaacagt tcgcgcttta ttgctccctg tggacctgtc cccatccgca
    18841 tacgatacca ttcgtaatca ctgacagcct gagtaccaag attacgtccc cggaatcgtt
    18901 ctccgggtat aacctgagta aaaatatcct tcggtacagg ctgccatcca ccatccagat
    18961 cctgcatata atccagcgtc attaacggcc acacactgtc tgcacgtact ggtgtctgtt
    19021 gtttatcaaa tccatcatgc agtaccagat gcccgtgctg gatccccatc cataatccag
    19081 cctttgccag agttctgtgc acatccaggc aactttccag ctcaccattt ctcgccattt
    19141 ccggtaagcg ttcggcaaaa tgacggcagg cgtactgttt cagactctcc gtttctgcca
    19201 ctttttccgg ccggatatca acgtttatgg cttccggatc ataacgaccg ggtgtcccca
    19261 cctgactgaa aatatctgtc ggtacaggct gatattcccc cagtttccgc cccagttttt
    19321 cagctgtcca tgacggtccg aacgaactga gtgcaacacc ttcccgcgct ctgtcccagc
    19381 catccttcac caccagttcc ccttcctgca tcgtgatata cagaccatca ctggccagcc
    19441 gtttatgcaa agacaaccag tcttgtgcgg gttcttcacg cagcccggca atcgacatct
    19501 gcgcaatata ttccctgaac gtctgctttt tcccgcgtcg ccaggcatta cggcgctcac
    19561 gtaccagtgc tgttttgcgc acaatacggt tgccgggtgc atgcacaaag cagccattat
    19621 ccggcgcgaa tccgtgctta agttcaagtt cacgacatgc acggctgagt ttttcctggc
    19681 tccaggaaag ccagttaata taaccagttt caggatgtac acgattcacg gcaacatgca
    19741 catgcagatt atccgtgtcc gtatgaacgg cagccacgta ctgatgtttt gagagttcaa
    19801 gagatttcag tgtgtgacgc acacagtcaa aaagctgctc aggacgcggg ctttcatgcg
    19861 caggccagga aagaatgtag tggaaaacgg ggtctgtatc attacgggaa aaaacagctt
    19921 tgtccgcctt gtactgcatc tcttcagcag ctgtttccag tgaattgcag ttatggaaac
    19981 aggttacgcc atagaaattt acccactgac tgccgtcttt catcacatca atcagggaga
    20041 caaatttttc gttacgcagg cgtgttgcat aatcaacaag ccgtctgaaa cggttgcggt
    20101 gagggacatc actcttgatt tcgctgtctt ctgtgagctc attttcctgc acatcatccc
    20161 gcacagacgt gtacgcaatc aggtcttcaa aggaagacct gccgtcacgt cttttgtgcg
    20221 gaataatggc attcatcagc agttatctca gcgtcccatt aagtctcttc gcagagcatt
    20281 gacatattca gtaatggcaa caagaacctc tgcatactcc ttatcaccag tgcgttttcc
    20341 ctccacaaac agatgcttct gaaggcggcc aagacgcata atctcatcaa tcatacggtc
    20401 gtcagtacgg cagttaacca ccttatccag tgaagagctt cgtataaatg ttgaaaccgt
    20461 ctgtccactt gactccgctt tttctcttac gatttcagcc tcatcctccg tgaatctcgc
    20521 cgtgaatttt acaattctct tcctgttctc actgcccgtt cgggttttct tctcactcat
    20581 ggtctgctcc ttaccggggt gtcggggcaa agccctgacc aggtggggaa tgtctgagtg
    20641 cgcgtgcgcg gtccgacatt cccacatcct gtcccgtttt tcaggccatt atagcctgtt
    20701 atttttgtgc atcatagtct tatgcagacg gcagaggcag ggcgcagccc tgccaggagt
    20761 cagtttttga tgataaaatc agacttcaac atgaaacgga gagaaagtgc caatggcttc
    20821 taaaaaattt tactcagatg atgacatcca gctggcaaaa gctgctttat cagaattgcc
    20881 ggacctcacc gctcaacgga aaacattgcg tgattttctt gatgccattc gtgatgacat
    20941 tattgtcctg gtaaggacaa agggttatac actggcagat gttcgtgaca cgctcaaaaa
    21001 tgcagggtat gaagtaggag agaaggcact tcgtgacatt attcgggaag cagaatcgaa
    21061 aaaaccatcc cgccgttctt cctctaaaac agcacctaaa aagacagatt caggcaggaa
    21121 tgatggcatc gatatgaaca acaattgatg tgacaatgtc acatcgtgtt ctgagattat
    21181 gatgattaca gaaaaaagcc ccggcataaa taccagggct tttctgttta atcagactcg
    21241 tggcggcaat gcaccaggat aatgccgtgc aataatatca ttcagttttt caacaaggtc
    21301 aggacgctta aagacaatgt gtacggtccc tttctggtag ccacgcaccg taaaatattc
    21361 cccttcaaac aactcaccga cattgtcgcc gttcccgaga aattcaccat actgcgcccc
    21421 gatgctgttc ctgaagtcag gaacgttgcg cccctccagc agataaaacg gacgtatcag
    21481 gtcatccagt gcctcccgtc cgctgcagtc cagcgtcaca cgcccgttac tccagcggta
    21541 cagcaagttt tcaataatga ttttctttcc caggcggcag ggattattgg ttctgtaatc
    21601 ccatgacaat ttccgccaga catcaatcag tccctgttca aatgtctgca tcttactggc
    21661 attcagatgg cgaaaagttg ccagtacatt gtccagggtg atttcaggac aggtgtcgct
    21721 catcagctgg ctgttccact catcacgctg gcatgaactc atgaatgtgt acatgccagt
    21781 atccgtcatc aggcgatccc agattttccg gtcggcaaga cgggtgatat tcttttttgc
    21841 ttcttccggt ttatccatgc atcgaacgga gtcagtcacg cagtcttcaa acccgtagag
    21901 aaaaatgtca ccggaagcat cgagcagaat cctgcgcgct gtcgccaggc attccatcgc
    21961 ctgcatgaac agtgcaatac cttcgttacg ctgcgcgatg atgcggtcga tagccacaga
    22021 cggaataaca tctgtcgtgc cggcagtatt caggtcagca gtgggtaaag tctgattcat
    22081 cacattgtct cctcatcgtc acgactgccg ggtcagccag gtcatttccc ggaggagcga
    22141 agctccttct gccgtccggg tcttcgggga tttccccgca gtggaccttt ccgatggggg
    22201 cccgtttacc gcctcacggg acagctgtcc gggggcggac gcaaaatttt ttgccgggtt
    22261 catggctaaa aattttgtgg ccggtgcatc tcaccctgga cagatggaac gggagggggt
    22321 tacgtctgcc ccccggaaag tccacagcgg aaacaggccg cagaggtggc ggcaggaggg
    22381 aagactgctg cgccatcagg cgcagtttcc tcacacaccg tcaggtgtgc agggagcgca
    22441 ggtcacggcg cgactgaaga aaaacgacgg cacaacaatg gcatgtcgtg gtcactggcg
    22501 caccactccc gcacaggtga aaacagagaa aaccgcacga catctataac taaaaaaagt
    22561 gaaatttctt cactttttta cacttattta tcgcgctgat ttggcaattg tgatgataaa
    22621 ataaagtgaa gttttttcac tttttcaaag gtattttatg accggatggg aactgcgaat
    22681 ctggcgaaaa agcatgctct ggtcacgcga aaaagcggcc agagaatttg gcgtgacgca
    22741 gagaacatgg cacgcctggg aaaatgcgga acaggtggat gtcaccgtct ggaggaccac
    22801 ccaggcactt tcagtccgtg acctgctgcc tcacatgcag gggatgcgca aggcagatat
    22861 catcaggcga ctggaaaatg aactggggga aacggcagag gatgtgtgag cgtgattcac
    22921 agcctggctg aatgccaggc tactgctcat caggaaacag ctttttcagt tcttcaccgg
    22981 aaagtccggt cgcctcctga atccgttccg gcgtccagcc catatcccga agccgccagg
    23041 ctatttcccg ctccacttca agagcgcctt tttgaataag acgttctgca atcgtcatgc
    23101 tgcttgctct gctcctgctg gcaaaaaagc ccgggatttt gccgggcttt gcggttctca
    23161 tcagtgagtg attttcttca gttcgtcatc ggaaagtccg gtagcctgct taatctgctc
    23221 cggcgtcatc cccattttct gaagctggca cgcaatagcc agggcttttt caagcgctcc
    23281 ttcctggcga cctttttgct caagacgttc tgcgatagtc atcagtttat cctcatgctg
    23341 cggcagacgc tcagccagcg cccgcataaa ttccggtgat acagagtccc ctgcgttaac
    23401 gatgtaatga atcagaacac gcacctgctc gtctgaaatt tccaccatga cctgcgggag
    23461 tttgtccagc agctccatca tgtcacgatg gcggatatgc ttcatcagca gcgtcagtgc
    23521 ggccatccgg cgatggttca tgatttcatt gtcatcaacg acagtgatat caaccagcgg
    23581 aaacggcttt gtgtatattt tagccgcaag agccggattc tcaaaacagc ccagccagtt
    23641 catgctgtac gggtacgggc tgcgctcacc gtgatagaac agcaccggaa tgaccagtgg
    23701 caatgttttg tgctgctcca gatgtctctg catcgccgcc acactgtaac gtaacatgcg
    23761 aaaaggcatg tacagatttt cggtgctctg gtgctcgatg agcgtataaa catagccgtc
    23821 ttcacccccg gtggttttca cgctccagag gatatcgctg gcgtactgat gcaggtccgg
    23881 ctctacaaag gtggcgggtt ccagcttcag cgtggacagg tcgcacaact gccggtactc
    23941 cgcaggaaga tgcagttcca gaaaatctct ggcgacgtcg ggattcgcca ggaacgaccg
    24001 gaacgcggca tcatgcggcg tgggcgttgt ggtgtttttc ttcttactca cggttcctcc
    24061 tggtgagatg tctgaacgta tcctgccgcc tgcacaggca gacggcaggg gttaatcagt
    24121 ggcagacccg ctcaatttca agcgcgcgga gcaggtggcc ccattcgctg actttcatgg
    24181 cggggatacg tacccgcttc tgctcactgc agcgtctggc gatatgaccg cccatcgcca
    24241 ggacgtccgg gttaatctca tccggtgcca gttcctggcg tctgaagcgg gtatggcggt
    24301 gcaggggttt gttctcccgc aggcactcct cacgcagacg ctttacatac tcggcggttg
    24361 cgaagataat tgatggtctc atggcataat ctccatagtt agtgaatgag atgccggact
    24421 tccgtacggg gaagcgccag cggagaaagg cagggtttat cgccctgctt tttttcattc
    24481 cggacagcga cgcagtgcca gcgcgagcgc tttcatcttc cagccttccg gcgtgcggta
    24541 catcaccttc cagtagccgt cgatgaacac ataatggaag cgcacctctt cagtgctttc
    24601 cgtggcgatg gcctccctga aagaacggta ctcacgcgcc tcactgtcca tccggaaacc
    24661 actgtcacgt ccgtaataag ccgtcacacc gtccaccggg ttctgaagcg tatgcccggc
    24721 gggtttgtcg caacggggac cgaggcaacg gatatcaccg tgttcaacca gggcttcggc
    24781 ctgctgaccg gtggcatagt gggtatgcag gatgcgaccg ttccaggtcg gctcaccgtg
    24841 caggtggcag taaacggtac ggaacacccc gtcgcggcaa aaaacggaaa tcgtgctgct
    24901 gactgtcatg aaaacgtctc cttctgctga atcaggtcgc cgggccgacc ggttgctccc
    24961 ccggaggggc gaaagcccct tctgccgtcc acttcttcgg ggtttctccc cgcagtggac
    25021 ctttccgatg gggcagcgac agccacggca cggggcagtt gtccgggggc agacgcaaaa
    25081 gtttttgacg cttgcggcaa aaagttttgt ggctggtaca tctcccccct ggacggctga
    25141 cacgtgttgt ggccgcttca gcccccggaa agtccacagc gggaaacagg ccgcaggggt
    25201 ggcggcggaa gggcagacaa ctgcgccatc aggcgcagtt tccttacaca ccgtcaggtg
    25261 tgcagggagc gcaggtcacg gcgcgacaga ggcggcgcga ccggaacggt cgctgcaagc
    25321 ctggtttttc agacatcgga ccgtagcggc a
//
LOCUS       DAZFXT010000065        25278 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.065, whole genome shotgun sequence.
ACCESSION   DAZFXT010000065 DAZFXT010000000
VERSION     DAZFXT010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 25278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25278
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.065"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..269)
                     /locus_tag="ACHKW0_002845"
     CDS             complement(<1..269)
                     /locus_tag="ACHKW0_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475647.1"
                     /translation="MKIKQHKEKLEIIRDIIRETLLGNAALIVLAAMVPVVLVKPWDS
                     MTFIFMGAVCVSFAVILFWLVYIVCLYIGTLVKNIFIRIFAFSIWA"
     gene            529..1350
                     /locus_tag="ACHKW0_002846"
     CDS             529..1350
                     /locus_tag="ACHKW0_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HFO7475648.1"
                     /translation="MKPNSTGIAARMMLSIDRARISEALLNNRQLQSTPLQIRYPQGV
                     REALGIMSEQLSLSVSDLTRILVEDALTEMFLPAENIVHRLICRTEHIMQAHDVCVTT
                     MTTLLAPWNIRPAVFRDPDKLTDYLTGEILTALADWFYLNPEWLNGRVRYPLSRAGEW
                     PATQDAFCQLISASDNVDIIIWHSFPFAGVHSQEYCGVLFRKKKEINKAIIYPVLSLS
                     PVRMNKEKENWFHTARNLYAKTSVRTVTLRPAQAEYLMTGKILPAELFSIPLLPW"
     gene            1440..2642
                     /locus_tag="ACHKW0_002847"
     CDS             1440..2642
                     /locus_tag="ACHKW0_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001532112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HFO7475649.1"
                     /translation="MMMNMHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKAGPEDDFSLLIPSVGGQLSDPGNISDNADKISDDWKAFDRAID
                     SQSNVSQTAARLKTRLQDFRHTHADAQVGTSAVATLPGDTLAAALMLKSYGTVSVDGK
                     VSDADLNYLESIANSNGQDVDKDRLTSQAFARAALVTDVGIALATELETAGQKWSLGF
                     TPKFQRVDLFNYNVLVKNYDSSAFKGDRYHNTRNGINADIGASMDLDDNWTLGLVAQN
                     LLPRSIETKEVNGVTETFRIRPQVTAGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AIGAEFNAWKWAQLRAGYRQNLASNDGSAFTAGLGISPFDVVHIDVAGIAGTDNNYGA
                     VAQLQFTF"
     gene            2782..2952
                     /locus_tag="ACHKW0_002848"
     CDS             2782..2952
                     /locus_tag="ACHKW0_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475650.1"
                     /translation="MFNKNKTHIENKAFMVEPFRDPTANECNVVIANDICIRVALSPS
                     EVLQPFSKNTNQ"
     gene            3001..3453
                     /locus_tag="ACHKW0_002849"
     CDS             3001..3453
                     /locus_tag="ACHKW0_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081199.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HFO7475651.1"
                     /translation="MNRMIPAGCLAVLCLNGCHLPPDNTAPTRKYGAASDVAVARHAQ
                     YQLWGQGIYSAALPERQSGGSLKAHSDRVSFDWEGDALELLNELARLRGQQFSYSGVR
                     LPLPVSLHVRDMTFADVLRLTEAQTAWRATLSQSAGRLHLSFMTPEKK"
     gene            3450..4286
                     /locus_tag="ACHKW0_002850"
     CDS             3450..4286
                     /locus_tag="ACHKW0_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HFO7475652.1"
                     /translation="MKTHLCLLLSALLLTGCAGQHHRSSAVMTENAPPPDIRAWLTPS
                     GNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISK
                     EGWLPPVIDEAQDVAHIRPDQIRTASRVWTILRPERFVSNPPGWRDWLLKGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRLNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQMQTVTGNGQKLITGDRVRRLKTHAEFTLQKSRWRPLVSTEGEKNDI
                     TR"
     gene            4270..5418
                     /gene="traJ"
                     /locus_tag="ACHKW0_002851"
     CDS             4270..5418
                     /gene="traJ"
                     /locus_tag="ACHKW0_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HFO7475653.1"
                     /translation="MISPDDFGTFPFSRFTAEEFRHFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSVNPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKAISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRHILKNFPDRKIVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRNTLSGQS
                     HATWGHYIDEIIRQEKRRIRDQVLTMYQNDRIEKSEAALFIPAGEFPR"
     gene            5415..5705
                     /locus_tag="ACHKW0_002852"
     CDS             5415..5705
                     /locus_tag="ACHKW0_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7475654.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFMLLGYSGWTVRTLCIRLYSLLQGKQASGRPWWYRHRTELPREWHGPE"
     gene            5769..9785
                     /locus_tag="ACHKW0_002853"
     CDS             5769..9785
                     /locus_tag="ACHKW0_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPD7 domain-containing protein"
                     /protein_id="HFO7475655.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVATRDD
                     KKGAKSGAYKAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQARYARSYINQLPQATEHEYLTRKGILAAPGVRLNNKNELVIPFS
                     NSRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDAGNMIDVAENARQIWTDSPFVFCADNDHQRKINKGVSSAQKAAASTH
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDAFQRAMNAQLEYIGIPTPNNDTTDRREA
                     IVIGNLIFTPVKDEKPQVYPENRQSTAPEPAVSEQNHPYDIPAEETDNQAYEDDYAAY
                     ASLQSVTAGTASPNEPQVNAVSAIDSNAKNTSAATTDDRNVLAQEPNADATVTTTAHD
                     TGTAETDSPSEPAEIQPVQQASPEPSAKDKPQENVVSATDNDAENTSSATTALRSYDT
                     PETSVSDATGTQHTATTQPEEPQQVSMGSADDWSAFAEDLNADAPGNATVHSTDNAET
                     KTLSEPAGMQPVQQATPEPTEENKPQEEGVMSATDTGEESAAAAIDNDLNTVAEDLNA
                     DVTVMTTAHHTDTAETASPAEPAEIQPVQQATPEPTAEDKPKEERPVSATETSTANET
                     ARPETPSLFSRLRRFLTGGKSRTVEPPVIQENTLTEAETATEAAAPPSPPETSPEDTS
                     SAEPDTIIFAPRRPNSPQRHNLDEIIKNLEHEELPDNTALYRVDGEPAFLDKLNRLEM
                     VDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIVEYDIKVRMKLPAQRA
                     ALEKLRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVSPKAEAEQTAGSTAET
                     PHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDTPEEEPPGKLRPGESV
                     TAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVKNHQKGDPVTLTLQAR
                     ETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFPMATFSQVMSQMQQHW
                     PQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAPNELIPAIASADKDNR
                     QLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINALTDNGLQFAGYGEAVN
                     HDENTHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSLGFEQTWKEWSDSQKA
                     EDRQEKTLQQAQSHSPGL"
     gene            9802..10152
                     /locus_tag="ACHKW0_002854"
     CDS             9802..10152
                     /locus_tag="ACHKW0_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HFO7475656.1"
                     /translation="MNMKKTLLVILTSISLASPAAVAATCRAGAAAEAGSKAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDVSITLPQFPSLDKILRDLENQVCDAVVDRVNE
                     HLPGNIDPWKDYNL"
     gene            10164..10859
                     /locus_tag="ACHKW0_002855"
     CDS             10164..10859
                     /locus_tag="ACHKW0_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HFO7475657.1"
                     /translation="MSEAHPTSPVSPETDNSGDIYKQTLSTIAQSEQRAALVPGLVRA
                     LLCSSAALVISLTGNIIQYWHNSNVTREYFATDNGRLVRLAPTSQPAWSQSDVMTFGS
                     MTLSEAFNLDFVHYRKQITTLAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTATIG
                     AGTLVNQGQMENGIWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAASGT"
     gene            10870..11844
                     /locus_tag="ACHKW0_002856"
     CDS             10870..11844
                     /locus_tag="ACHKW0_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotH/IcmK family type IV secretion protein"
                     /protein_id="HFO7475658.1"
                     /translation="MQIHHLLGALLIVCGTVTAADNPGWQNARTPQAGGKTGQTPGSA
                     PAGGPPPATQLTGPLPPPASPLVQEAASLDSPLNAGEIRELRGRMADNERAISAPVTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDINGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTNTLSQEIDSRLDLRIPR
                     QGPASPVVAVPSEKIALHDATLQAFLDGVPPDDPTVRRLKFSGNVPGTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSIHVTPELE"
     gene            11848..13179
                     /gene="traO"
                     /locus_tag="ACHKW0_002857"
     CDS             11848..13179
                     /gene="traO"
                     /locus_tag="ACHKW0_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HFO7475659.1"
                     /translation="MSAEQDTAAKSGKKLSALLVLGGVIIIGGGYLLLTWLSPENSAS
                     LSSVNINSTTTGSGRTVVESQHYRDLLRADNERGAAEALRNNQTFIASLPRGLDTDHG
                     NNQRRDTQPADQAEGSGQPSTPPQADQTAREKQREALQKLLARINTQHPDARPPVVAA
                     AMWGTTGAPGISSHASPPGTGTSPQPRLQEASLATPAARTGIQLIPALTRVPAFIDTE
                     VDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGKGMDIHFDRMMWRGMFLKVNAYALN
                     EHTGMSSVASDVNHRWFKHIVLPSVLGGVGSVGSLYKDANTQVIQGNYGSITGRVGMP
                     DGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDISPPS
                     SGTAPSVPPPSGRPLTQEEAERQASDRIQAAVMRKQNEMRQRYGIQQESHP"
     gene            13176..13889
                     /gene="traP"
                     /locus_tag="ACHKW0_002858"
     CDS             13176..13889
                     /gene="traP"
                     /locus_tag="ACHKW0_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HFO7475660.1"
                     /translation="MKNEHEDPGSFGETTTPASGKSFLKRQYMGYSLPVWAGCVLIIL
                     AAVWYKFLRTPSEPPADAGFAVTPPAAESWAEPMPMTETGSGNTMSAPPVSPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKESITTLGDTIRKDEAWAQETRQLLDETRKQVHAL
                     QMQGPLQIPTGNNGQSTKTPPSRLQGMQIMSVEKGMAWIRWQGSAWAVREGDRLGNIT
                     ITRIEPSSRSVITSAGTLR"
     gene            13886..14416
                     /gene="traQ"
                     /locus_tag="ACHKW0_002859"
     CDS             13886..14416
                     /gene="traQ"
                     /locus_tag="ACHKW0_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HFO7475661.1"
                     /translation="MMSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRA
                     RAEGRHSMGPGKFIAGIVICGMLVALPSFINAGGTQMGFRADSFGPIAYVQPTTFGAA
                     AGAANAMLSLVKLAGVGFAMNGISIWRKSLLDGHTALSSSESISKGNVKFVAGVLLVF
                     IDRVIDASLASLGLAF"
     gene            14465..14863
                     /locus_tag="ACHKW0_002860"
     CDS             14465..14863
                     /locus_tag="ACHKW0_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6750 family protein"
                     /protein_id="HFO7475662.1"
                     /translation="MSRFRAMAARLCAAFIYVQLCLVACRDRLIRLLLLLPVILLPHT
                     AMANTLFDMIKTVGDGAKGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGLCLAVVPELMGSGQKTLGLSPVSIS"
     gene            14920..15171
                     /locus_tag="ACHKW0_002861"
     CDS             14920..15171
                     /locus_tag="ACHKW0_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HFO7475663.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKTYEQNTATHRPGPGTLAFPAPATGHGIQR"
     gene            15086..15904
                     /locus_tag="ACHKW0_002862"
     CDS             15086..15904
                     /locus_tag="ACHKW0_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HFO7475664.1"
                     /translation="MNKTPPLIAPVRERWLFPLPQPDTVFSGESLLPPPVLSTPGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTVVLLEKNLTRVMRQKKHPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESELLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALKEIFSDERKRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            15963..16322
                     /locus_tag="ACHKW0_002863"
     CDS             15963..16322
                     /locus_tag="ACHKW0_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475665.1"
                     /translation="MDTHSAGYQFTADSFRRFIIRTLTDNIPPDGDEKEIARFILRHP
                     AFNGGWLLFLAAEIHERSRTHKSITVTVSVDNYLSWMDIAVSGLPELPDMPFCDFFIR
                     RQQFIYPGVPPTKSGEE"
     gene            16322..16702
                     /locus_tag="ACHKW0_002864"
     CDS             16322..16702
                     /locus_tag="ACHKW0_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475666.1"
                     /translation="MKIHRISPETLITLIHAHLAGKTDSTAKEEHRLLRRFLRDDDGR
                     LAGVLLNIAGILQFNRELSARHNYPATPLTEFSLRKRGKQLHLCLCSLRFFYIPPVFI
                     QNKRRKSIVVHLNKITYKQTHSIR"
     gene            complement(16675..17220)
                     /locus_tag="ACHKW0_002865"
     CDS             complement(16675..17220)
                     /locus_tag="ACHKW0_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475667.1"
                     /translation="MKAENKILNLFHATTMSRGLQIIKDGKIRTDAPSVICWSLETTP
                     GWIYLSDQLDNAVHWGNKIACLSKDELFFYIFRVQVQEENCYPDTDDLQYVSWLSPEE
                     AENTDLYTCLSLCHSCRTDKELYTGCEISDYAVLPATTLYTDNSGHPQYDIVNTLKKH
                     IGKDEYNQYESLRQRIEWVCL"
     gene            17445..20489
                     /locus_tag="ACHKW0_002866"
     CDS             17445..20489
                     /locus_tag="ACHKW0_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7475668.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDLTAPGTLESLITALAERMNTAY
                     KNTGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKDHQTRIRKLAEHAPPARFAQDPWRWTLSALKIRHDAFLD
                     TLEQALTHDSDGLLVRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGED
                     TSVFHAPSLNLQLFTTQPETHGSLIKAGDLWHGMVAITLPPQNLRTFNQLVRDVPHAI
                     PWRIRMDLMPGGMKALGVKKTLLTYSSFISPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHGSGPVPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PEHTRNLFDVMYGAKKPATPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISNLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHMAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSAELLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQDYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQELGSLRARQI
                     LAENFPRGSATSLIEHRARTHDSENVIHDLAAELIRKQGYHL"
     gene            20489..21109
                     /locus_tag="ACHKW0_002867"
     CDS             20489..21109
                     /locus_tag="ACHKW0_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HFO7475669.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGVRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     SPLTALMSDNTWLWPNSPGTPALTAGDWPVMETDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESHSHRVPDILYPLKTEKKECKDNQQPHCLHSW"
     gene            21067..22272
                     /gene="traW"
                     /locus_tag="ACHKW0_002868"
     CDS             21067..22272
                     /gene="traW"
                     /locus_tag="ACHKW0_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HFO7475670.1"
                     /translation="MQRQPATTLLALLVGCAVIPSTHAYSVTVVASQPVTTQIMPELA
                     AIQGVMAEMVRMQTATGTAINQSSEKLASVIAQDGQATRQQMIFSNETRRLEEARKSF
                     SVPDSICSESASGVAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPTREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGELPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMSQGSVR"
     gene            22269..22838
                     /gene="traX"
                     /locus_tag="ACHKW0_002869"
     CDS             22269..22838
                     /gene="traX"
                     /locus_tag="ACHKW0_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HFO7475671.1"
                     /translation="MKDDNSTDKKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     GQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVNELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPAITLIRILACLLLMSAIAALCASKTLIVSYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            22911..25076
                     /locus_tag="ACHKW0_002870"
     CDS             22911..25076
                     /locus_tag="ACHKW0_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HFO7475672.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIAIMGIVWFLFIGIRHVVRTGHQGNVFNN
                     GRDVVGMLSIVSGFMMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMALCKHAMNAGLDDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLTVKGNGSNKQSALDYFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSQGEAGDTEFLKEVKGAYQ
                     AQLQNSPYTPALGNLTSSDEIYAANTNDPQSAIIKLMSPDFTKLTYKIATVYSSTGTT
                     SYQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAIFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNIRRTPGVREEKLKNINRDDKDGIKQ"
     gene            25163..>25278
                     /locus_tag="ACHKW0_002871"
     CDS             25163..>25278
                     /locus_tag="ACHKW0_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutE"
                     /protein_id="HFO7475673.1"
                     /translation="MNEQRSRYPSWWAYYYLVRNAYFLFGVPFLIFCAIAFTA"
BASE COUNT         6259 a         7306 c         6432 g         5281 t
ORIGIN      
        1 gcccaaatag aaaaagcaaa aatccttata aaaatattct ttacaagcgt tcctatatac
       61 aaacaaacta tataaaccag ccagaacaga atgactgcaa aagaaacaca cacggccccc
      121 ataaaaataa atgtcatcga atcccatggt ttcaccagta caaccggaac catggctgcc
      181 agaacaataa gagcagcatt acccaacagc gtctctcgaa taatatccct tataatctcc
      241 agtttttctt tatgctgttt aattttcata cgaacctcgt tgatttcatc ccgaaaagga
      301 aaagattata taacgaaata ctttcaatct cctgactccg tctgctctgc agcataatgc
      361 cagaccagcg gcatgctaaa tcacgtcctg tccgaatcct tttcattcac gcttaaaaca
      421 gagttaaaag cactcttgaa acacactcga tcataacgat cgaaataact cgtttccgtt
      481 cagtaaacac gctctttaga cttaacatat gaaccgcacg gaggtgaaat gaaacccaac
      541 agcacaggca ttgctgcccg catgatgctc tccattgaca gggcccggat tagcgaagct
      601 ctgctcaata accgccagtt gcagtccact cccctgcaga tccgttatcc gcagggcgtc
      661 agagaagcac tcggaataat gagcgagcaa ctctcccttt ctgtatccga cctgacgcgt
      721 atcctggtcg aagatgcatt aactgaaatg tttctcccgg ctgaaaacat cgttcaccgt
      781 cttatctgcc ggacagaaca catcatgcag gcacatgatg tctgcgtaac aacgatgaca
      841 acgttactgg ctccctggaa tatccgtcct gccgttttcc gtgacccgga caaactcaca
      901 gattatctga caggagagat tctgactgct ctggctgact ggttttatct caacccggaa
      961 tggctgaacg gcagggttcg ttatccttta agccgtgccg gtgagtggcc agccacacag
     1021 gatgcgttct gccagcttat ttcagccagc gataatgtgg atattattat ctggcacagt
     1081 tttccttttg ccggtgttca ttcacaggaa tattgcggtg tactgttccg caagaaaaaa
     1141 gaaattaata aagccatcat ttatcctgta ctttctttat cacctgtaag aatgaataag
     1201 gaaaaagaaa actggtttca cactgcccgc aatttatatg caaagacatc cgtccggacc
     1261 gtaacgcttc gtccggcaca ggcagaatat ctgatgacag gaaaaatatt acctgctgag
     1321 ttattcagta taccgttatt accctggtaa taacgcagag aaaaaaccag taaattttta
     1381 atggcacagt cgtgtcatac cgttgctgta cccaaaacac ctcaaaccag gaaactgaaa
     1441 tgatgatgaa tatgcacatt acccgaactc tcatcgcatc tgctgtttta ttttccttta
     1501 attcaacagc cgcaacttca tattttgaag cccgcaatga tgcaatgggg ggaaccgggg
     1561 tagcctcctc tcactacggt gtggcacctc tggcaaaccc ggccctgctg acaaaagccg
     1621 gtccggaaga tgacttcagt cttctgattc cgtccgttgg tgggcaattg tccgatccgg
     1681 gtaatatcag tgacaacgca gacaaaatca gtgatgactg gaaagccttt gatcgtgcca
     1741 tcgacagtca gagcaacgtg tcgcagactg ctgcacgtct gaagacgcga cttcaggact
     1801 tccgtcacac gcatgctgac gcacaggtgg gaacatctgc tgtggcgact cttcccggtg
     1861 acaccctcgc tgccgctctg atgctgaaaa gctacggtac ggtcagtgtc gacggtaaag
     1921 tcagtgatgc cgacctgaac tatctggaaa gcattgcaaa cagtaacggt caggatgtgg
     1981 ataaagacag actgacctct caggcttttg cccgtgccgc actggtcacc gatgtgggta
     2041 ttgctctggc tactgaactg gaaacggccg gacaaaagtg gtctctgggc ttcacaccaa
     2101 aattccagcg tgttgacctg tttaactaca acgtgctggt taaaaattac gacagcagcg
     2161 ctttcaaagg cgaccgttac cacaacacca gaaacggcat taacgctgat atcggggcca
     2221 gcatggacct ggatgacaac tggacactgg gtctggtcgc acagaacctg ctcccccgca
     2281 gtattgagac aaaagaagtg aacggtgtca ctgaaacttt caggatccgc ccgcaggtga
     2341 ctgccggtgt ctcctggcac aacgacatgt tcactacagc actggatgtt gacctgaccc
     2401 cggccagtgg cttcacatca gacagtaaac gtcagtttgc tgccattggt gctgaattta
     2461 atgcctggaa atgggcacag ttacgtgcag gctaccgcca gaatctggcc agcaatgatg
     2521 gcagtgcgtt caccgccggg ctgggtatct caccatttga tgtcgtccat attgatgtgg
     2581 cgggtatagc aggcacagac aacaactacg gtgccgttgc acaactgcag ttcacattct
     2641 gatcatctct gccccggtac tgaccggggt atccagacct gcatcctcgc gttttccgac
     2701 catcaaacga cacagccaga cctgtagtgc tgcctctgat ctggctgtta tctgacatat
     2761 cattttcgca tgaggaaatg catgtttaac aaaaataaaa cgcatatcga aaacaaagcc
     2821 tttatggtgg aaccgttccg ggatccgaca gctaacgaat gtaatgtcgt gatagcaaac
     2881 gatatttgta tccgggtagc actgtcaccc tctgaggtgc tccagccatt cagcaaaaac
     2941 acgaaccagt aaatcctccc cacaacgctt tcttctttca ctgctcagga aaaatacacc
     3001 atgaaccgta tgatacctgc gggatgtctc gccgtactct gcctgaacgg ctgccatctc
     3061 cccccggata atacagcgcc gaccagaaag tatggtgctg cctctgatgt cgccgtcgcc
     3121 cggcatgcgc agtatcagct atgggggcag ggaatttact ctgcagccct tccggagcgt
     3181 cagtccggcg gcagtctgaa ggctcacagc gaccgtgttt cctttgactg ggaaggcgat
     3241 gccctggaat tgctcaacga actggcccgt ctgcgcggcc agcagttcag ctacagcggc
     3301 gtccgtctcc ccttacccgt ctcactgcac gtccgcgaca tgacctttgc tgatgtcctt
     3361 cgtctcactg aagcccagac tgcctggcgg gcaacactct cgcagtcagc cgggcgttta
     3421 catctgtctt ttatgacgcc ggagaaaaaa tgaaaacaca cctgtgtctc ctgctgtctg
     3481 ctctcctgct tacaggctgt gccggacagc atcaccgttc ttctgccgtc atgacagaaa
     3541 acgcaccgcc cccggatatc cgtgcatggc tgacgccctc cggcaaccag gatgatggta
     3601 tttcagatac ccgtcgtcag atgctcacgg atgccggcag aacgctggga ttccgtggtg
     3661 gaaaagcaca gcgctcatgg gaactgattc aggcactgaa tgcccgggaa agtaccctga
     3721 atgccctgta tgatttccgg cctctcatca gcaaggaagg ctggctgcct cctgttatcg
     3781 atgaagctca ggatgttgca cacatcagac cggaccagat aagaacagcc tcccgtgtct
     3841 ggactatcct ccgcccggaa cgctttgtca gtaatccacc tggatggcgt gactggctgc
     3901 tgaaaggact ctcaaccacc gccacaccag gcactgaagg ccgggttgtg ccggaagacc
     3961 gggcacaacg cagactatgg gaaaacgcct tacgtcaggg atggcaggaa gggcgcctca
     4021 acgcagatct cacactggaa gctaaccaga agcgcctgac ccgtgattac cggggcatga
     4081 tgctttacgc ccttctgtgg cgtcagggca tgatcacacg tccagatgtc acagagcaga
     4141 tgcagacggt taccggcaac gggcaaaaac tcatcactgg cgatcgcgtc cgccgcctga
     4201 aaacacatgc tgaatttacg ctgcagaaat cgcgctggcg ccctcttgtc agcaccgaag
     4261 gagagaaaaa tgatatcacc agatgacttc ggtaccttcc ccttctcccg ttttacggca
     4321 gaagagtttc gccatttttt tgcctggtgt gcccgccacc gcgtcagtga tattgatctg
     4381 accggcggtt caccggtatc cgtcagtcgc tttggacggc gggttcgctc atccgtaaat
     4441 ccccttccgt cttccatcat gatggcactg agtgatgagc tgtttgggcg ggaggttctt
     4501 ccccgggttc tgtcaggtca tcccgtggat cgtaccattc aggtcagcgg agatgcctcc
     4561 ggccgctatg gcctgcagcg aggtcagcgt gttcgcctgc gctgccacct gatacaggga
     4621 acatcggcaa cagaagaaaa agccatttcc gtcaccatgc gggtgatccc gtcagatatt
     4681 ccggacatcc tcactatgaa tattgaaccg gatctgctgg atgccatgct cagaaaaacc
     4741 ggcctgggct tcatctgcgg agaaaccggc tccgggaaat ccacactggc ggcggcactg
     4801 tatcgccaca ttctgaaaaa tttccccgat cgcaaaatag tgacctatga agatccggtg
     4861 gaatacattc tgggcggaga gaatgacctt ctgcctcccc atcaggccga aatcgggcgc
     4921 gatgtggaaa gttttgctgc aggacttcgc tctgccgttc gccgtaatcc ggacatcatt
     4981 ggtatcggtg aaatcaggga ttatgaaaca gcagatgccg cagtccaggc gggtaatacc
     5041 ggacactttt gtatcggcac catgcacaca aaatcacccg gagaaacctt tgcccgcctg
     5101 ctgggattgt ttcctcctga aatcagggat tcgatggctg cagccacgct ttccctcgta
     5161 cagttcattc tggtgcaggt actggtcaga acaaatgacg gcggcaggca ggctgtccgg
     5221 gaatacatcg tcataactga cgaactgcgc aatacgctct ccggacaatc ccatgcaaca
     5281 tggggacatt acattgacga aatcattcgt caggaaaaac ggcggataag ggatcaggtt
     5341 ctgaccatgt atcagaacga ccgcattgag aaatcagaag cagcgctctt catcccggcc
     5401 ggagagttcc cccgatgaaa cgcgaaggct tctggcggga tgtaacacca ccgctcaaca
     5461 tttatggtgt ccccctgccg tttaccatga tttacctgct gttgtttccg tatccgtcaa
     5521 aaacgacatt ctgggtgtgc agcggcattc tgctgttttt tatgctcctg ggatattccg
     5581 gctggacagt ccgcaccctc tgtatacgtc tgtacagcct gcttcagggt aagcaggcat
     5641 cgggacgtcc ctggtggtac cgacaccgca cagaattacc ccgtgaatgg cacggaccag
     5701 aatagccctc ttttgtccat catcccggcg tatcacaacg ccctcatatt taatccccgg
     5761 agaacgacat gaagtccaga tatcgtacct ggcttgctgt cccgcctgag gagacagaag
     5821 ccgtaaaaaa tgcagtcccc ccgttaaatg gccgcaaagc tgtcgcatgg gatccagaaa
     5881 aaaaactctg gtatgcccgg gcgggtaccg aactttcact gctggagcgc tggttacccc
     5941 ggccgcagga actgtcgatg gatgccggcg atccggtcac cgaatttgct caggtcctgg
     6001 aaaacgccgg actggttatt cagggattgc cacagatgga tggcgccata cacagagtcg
     6061 ccacccggga tgacaaaaaa ggggccaaaa gtggcgcata caaggcctat cttgatgggc
     6121 gccccgccgg ctggtaccgc gactaccgga gcgccgatga cagtcccaca aactgggtat
     6181 tttccggcgg agaacaacac gatcctctgg ccagactgca tctgcgggca ttcgctcagc
     6241 aacagcgcga tgataacgca cgtaaactgc agcaacagta caacaaacag gcccggtatg
     6301 cccgctccta tattaaccag ttaccacagg caacagagca tgaatacctg acccgaaaag
     6361 ggatccttgc cgcaccgggc gtccgtctga ataacaagaa tgaactcgtc atccctttca
     6421 gtaacagccg gggagaaata cgttcatacc agcgtattcc tgtcaccggc gggaaggatg
     6481 cccgcattct gaaagactca gagaaaaccg gtaactggtt caccttcgga accccggaaa
     6541 atggccgtcc cctgttattt gcggaaggtt acgcaactgc agcctccctg cacgaagcca
     6601 ccggccttcc ggttctgatg acagttgatg ccggcaatat gattgacgtg gcagaaaatg
     6661 cccgccaaat ctggactgac agcccctttg tcttctgtgc cgacaacgac catcaacgga
     6721 aaattaacaa aggtgtttca agtgcccaaa aagctgccgc aagcacgcat ggcgaagtca
     6781 ttattccggc ctttacggag gcggaaaaag cacagggact gaccgatttc aatgacctgg
     6841 acgccagcag gggacgagac gcctttcagc gagccatgaa cgcacagtta gaatatatcg
     6901 gtatccccac gcccaacaat gacactactg accgccgtga agctatcgtg atcggcaacc
     6961 tgatattcac accggtaaaa gatgagaagc cacaagtgta ccctgaaaac agacagagca
     7021 cagcccctga accggcagtg tcggaacaga atcatcctta tgacatccct gcagaagaga
     7081 cagacaatca ggcatatgaa gatgactatg cagcatatgc atccctgcag tcggtaactg
     7141 cagggacagc ctccccgaat gaaccgcagg tgaatgctgt ttctgccatt gacagcaacg
     7201 caaagaatac ctctgccgcc accactgacg accgaaatgt attagcacaa gaacccaatg
     7261 ctgacgccac agttacgacc acggctcacg acaccggcac agcagaaaca gattcacctt
     7321 ctgaacctgc agagattcag ccagtgcagc aggccagtcc tgaaccatcg gctaaagata
     7381 aaccacagga gaacgtagtg tctgctactg ataatgacgc agagaacacc tcttccgcca
     7441 ccactgccct ccggagttac gacacaccgg aaacatccgt ctccgacgca accgggacac
     7501 agcacaccgc cacaacgcaa cctgaagaac cacagcaggt ctccatgggc agcgctgacg
     7561 actggagcgc atttgcggaa gacctcaatg ctgatgctcc ggggaatgcc actgttcaca
     7621 gcaccgacaa cgcggagaca aagacacttt ctgaacctgc cgggatgcag cctgttcaac
     7681 aggcaacccc tgaaccaacg gaagaaaata aacctcagga agagggcgtt atgtctgcca
     7741 ctgatacagg tgaagagagt gccgctgccg ctatcgataa cgacctgaac acagttgcgg
     7801 aagacctcaa tgctgatgtt actgtgatga ccacagctca ccacaccgat acagcagaaa
     7861 cagcctcacc tgctgaacct gcagaaattc agcccgtaca gcaggcaacc cctgaaccaa
     7921 cggcagaaga taaaccgaag gaagagcgtc ctgtgtctgc cactgagacc agcacagcga
     7981 atgaaacagc ccgccctgaa acaccatcgc ttttcagccg cctgcgccgt tttctgaccg
     8041 gtggaaaatc acgtaccgtc gaacctcccg ttattcagga gaacacgctt acggaggccg
     8101 aaactgcgac agaggccgca gcaccaccgt caccacctga aaccagcccg gaagacacat
     8161 cgtcagcaga accggacacg attatctttg cgccccgccg gcccaacagt ccgcagcggc
     8221 acaacctgga cgagattatt aaaaatcttg agcacgaaga gttacccgac aatactgcgc
     8281 tctaccgcgt cgacggcgaa ccggcattcc tggataaact caaccgtctg gaaatggtgg
     8341 atggtgccag tgctgatgac aaaaaggtcc tggctgccct tgctgtggcc accaacttct
     8401 atggcggcgt tatcgagctg accggcagtg atgcctttaa acaaaaagcc atgcagctga
     8461 ttgtcgaata tgacatcaag gttcgcatga aactcccggc ccagcgggca gcactggaaa
     8521 aactccgcaa ggaaaaagag atggcgcatg atgccattgt cacccacaga cccacgccgg
     8581 aactcaaccg ccagtcaccg gaagagacaa caatggccca accggctccc cctgtgtcac
     8641 cgaaggcaga ggctgaacaa acagcaggca gtacggctga aacaccgcat ccggaagcgc
     8701 cggtcacggc aaaagctgaa gccccccggg ccgacagacc ggaacaaccc gccgccccgt
     8761 cgtctgcgcc agaaaaagcc cctgtcagtg cacagccgca gcaacccgac acaccggagg
     8821 aagagccgcc cggcaagctc agacccggtg aaagtgtgac tgccgtcctg acaaactacg
     8881 gagaaaaaga gtatgcgccc ggaaaagggc gctgcttttt tgtggagctc accaaccgca
     8941 gtggcaaacg ggaatactgg ggaaacggcc tggaagaact ggtcaaaaac catcagaagg
     9001 gtgaccccgt cacactgaca ctgcaggcga gagaaacatt ccggctgtcc ggtgaaacgc
     9061 gggatcgtat ccgtaatatc tgggccataa ccccggtcag taacggcatt accgttaata
     9121 atgaccaacc tcatgcgggt cagcataccc tggagtttcc gatggcaaca ttcagccagg
     9181 tgatgtcaca aatgcaacaa cactggccac agataatgca ggaagttcgc tcccccaaaa
     9241 atcctcccaa taatctgtac attggtgaag acaggcaacc tgcatcagca cctcaggatc
     9301 aatcaatgcg actccccctc gctggtaatg cccccaatga actgatacca gccatcgcct
     9361 ctgccgataa ggacaatcgt cagttgaatc ttctgctggt ccacagcgca gatgatcatc
     9421 tgcagggcgt ggtccgcctg aacggcaccc tttacccggc actggccacc ccgtcagctg
     9481 acaaccgtca gctggtcatc aatgccctga cggataacgg tctgcagttt gccggttacg
     9541 gtgaagctgt taaccatgat gagaatactc atcaacgtcc gtccccacaa ataatgcagt
     9601 ttcacctgaa gcaacaggac tccccgttgt ttgccgccat ccacaaaccg gaagagcaac
     9661 cggacaagct tttccgctct ctgggttttg agcagacctg gaaagagtgg tctgacagcc
     9721 aaaaggcgga ggacaggcag gaaaaaactc tgcaacaggc acaatcccac agccccggcc
     9781 tgtgagccgg taaggacgac catgaacatg aaaaaaacgc tgctggtcat actgaccagc
     9841 atctcgctgg cttctccggc tgccgtggca gcaacctgtc gggcaggtgc tgccgcagaa
     9901 gccggcagca aagccgcctg ggagcgggcg cgcacagcca gccaggcatg gtcacagcga
     9961 gaaaacgacg tcgcctcctc cctgcagtcc tgcctgtccc gcattaagga cgtcagcatc
    10021 actcttccac aattccccag cctggacaaa atccttcgcg atctggaaaa ccaggtctgt
    10081 gatgccgtcg tggatcgggt gaatgaacac ctgccgggga atatcgaccc ctggaaagac
    10141 tataacctct gaacaggagc cccatgagcg aagcacaccc cacatcccct gtctctccgg
    10201 aaacagataa cagcggagat atttataaac aaacgctcag taccattgca cagtcagaac
    10261 aacgggccgc actggttccc ggactggtca gggccttgct gtgtagcagc gcagctctcg
    10321 tcatcagtct gaccggcaat atcatacagt actggcataa cagcaacgta acgcgtgaat
    10381 actttgccac cgacaatggc cggctggtcc gcctggcccc caccagtcag ccagcctgga
    10441 gtcagagcga cgtcatgacg tttggcagca tgacgctgag cgaagctttt aatctggatt
    10501 ttgtccacta caggaaacaa atcacgacac tggccccccg cttttccgaa gccggttttg
    10561 ccggctatgt gaatgctctt caggcctcca acattctgga caccattaaa aaggagaaat
    10621 ttaacctcac ggccaccatc ggggccggca cgctggtgaa tcagggccag atggaaaacg
    10681 gcatctggtt ctggaaattc cagtatccgg tcaggatgcg tctggtcggt cagaccagca
    10741 cccgccccga gcaggccttc acctttgaaa tcactgtccg gcgcgttgac ccacgaatca
    10801 aacccggcgg catggaaatc agccagatgg tttcccgtaa cgccgcatca ggaacctgag
    10861 actcatatca tgcaaataca tcatcttctt ggcgcactgc ttattgtgtg cggcactgtc
    10921 acggctgcag ataacccggg ctggcagaac gcacgaaccc cacaggctgg cggaaagaca
    10981 ggacagactc ccgggagcgc ccccgcaggc ggccccccgc cagccacaca actcacaggc
    11041 cccctgccgc cacctgcctc tcctcttgtt caagaagccg catcactgga ttcccccctg
    11101 aatgcaggtg aaattcggga actgcgggga cgaatggccg ataacgaacg ggccatcagc
    11161 gcacctgtca ccagtgtggt gccccgtatc agctccctca cggtaaatct gtcacccggg
    11221 gccagtctgc cgcttgtccg aaccgcactg cataacgtca gcgtggtaac gttcacggac
    11281 atcaacggct ccccgtggcc tcagtcggat ccgccttata acgcggcacc cggtcacttt
    11341 gatgtgcagt tcaacggcaa tatggtctcc gtcactcccc tgcgcgcctg ggctgcaggg
    11401 aatgtaacgg tctacctcaa aggactgtct gtaccggtca ttctgaatat caccagtggt
    11461 gaaaccgaca ccaataccct gagtcaggag atcgacagcc gtctggatct gcgcattccc
    11521 cgtcagggac ctgccagtcc tgtggtagcc gtaccatcag agaagattgc cctgcatgat
    11581 gccaccctgc aggcctttct ggacggtgtt cctcccgatg atcccaccgt gcgtcggctg
    11641 aaattctccg gaaatgttcc gggcaccacc atctggcagc acggcgatga tctgctcgtg
    11701 cgatcgcggg ccatgctgcg ggatgaattt gaacagacgc tggcatctgc tgacggtacg
    11761 cgtctgtgga aactgccggt gactccctta ctcaccttct ccgtgaatgg tcagtccatt
    11821 cacgtcaccc ctgaactgga gtaaatcatg agcgcagaac aggacacagc cgcaaagtcc
    11881 ggcaaaaaac tgtcagccct gctggttctc gggggcgtga ttatcatcgg gggcggatac
    11941 ctgctgctca cctggctttc ccccgaaaac agcgcatcgc tctcgtcagt caatatcaac
    12001 agcacgacaa caggcagcgg gcggaccgtg gtggagtcac agcactaccg ggatttactg
    12061 cgtgctgaca atgagcgcgg ggccgcagaa gcgttacgta ataaccagac gttcatcgcc
    12121 agtctgccca ggggactgga tactgatcac ggaaataatc aaagaaggga tactcagcct
    12181 gctgaccagg ccgagggttc tggtcaacca tcaacgcccc ctcaggcaga tcaaacagcc
    12241 cgggaaaaac agcgggaagc gttacaaaaa ctgcttgccc gcatcaacac acagcatcct
    12301 gacgcccgcc ccccggtagt ggcggctgca atgtggggta ccactggtgc tcccgggata
    12361 agctcacacg cctcaccacc cggtaccggc acatcaccac aaccacgtct gcaggaggcc
    12421 agtctggcaa ccccggcagc tcgtacaggg atacagctga taccggcact gacccgcgta
    12481 cctgccttca ttgatacgga agtggactct gacaatgctg tctcccgcgt ggtggcctcc
    12541 gtacctgccg gcccatgggc cggggccgtc ctctacagcc aggacgccag actcgccggc
    12601 aagggaatgg atatccactt tgaccggatg atgtggcggg gcatgtttct gaaagtcaat
    12661 gcatatgccc tgaatgaaca taccggcatg tcatccgtgg ccagcgacgt gaaccaccgc
    12721 tggtttaagc atatcgttct gccgtctgtg ctcggtgggg tgggcagtgt cggaagtctt
    12781 tacaaagacg ccaacacaca ggtcattcag ggaaactacg gcagcattac cggcagggtg
    12841 ggaatgcctg atggcgaggc gctggctggc gtcattgcag gcggcatggc cgaacggggc
    12901 agtcagttgc tgacccgcca ggcggagagc gaaccttacc ggcaggtgac agtgtcacgc
    12961 ggagaggttg tctccattct gtttgttgag ccggtcatga gcaacgatat cagtcccccc
    13021 tcctccggca ctgccccttc tgtgccaccg ccctccggac gcccgctcac ccaggaagaa
    13081 gcagagcgtc aggcgtctga caggatccag gcagctgtca tgcgtaaaca gaacgaaatg
    13141 cggcaacgtt acggaataca acaggaaagt catccatgaa aaacgaacat gaagatcccg
    13201 gcagttttgg cgaaacaacg acaccggcat ccggcaaatc cttcctgaaa cgccagtata
    13261 tgggatacag cctgccagtc tgggccggct gtgtcctcat catcctggcc gcagtctggt
    13321 acaagtttct gcggacaccc tcagagcccc ctgcagatgc aggcttcgct gtcacaccgc
    13381 ccgcagccga aagctgggca gagcccatgc ccatgacgga gaccgggagc ggaaacacca
    13441 tgtccgcgcc tcctgtctcc ccacaggcag acatgcagca gcttgcccgt gacctgaaag
    13501 cagagctgga caggcgggat gaggaaatcc gacgtaccct aaccatgctg aaagagagca
    13561 tcacaaccct tggcgacacc atacggaaag atgaggcctg ggcacaggaa acccgtcagt
    13621 tgcttgatga aacccgtaag caggtgcatg ccctgcagat gcagggacca ctgcaaatcc
    13681 cgaccgggaa taacggccag tcaacgaaaa ccccgccttc ccgcctgcag ggcatgcaaa
    13741 tcatgtcggt ggaaaaagga atggcctgga tccgctggca gggtagcgcc tgggcagtac
    13801 gggaaggtga caggctgggg aatatcacca tcacccggat tgaaccgtcc agccgctcag
    13861 tcatcacctc tgccggtaca ttacgatgat gagcattgat gccctgacag ccatagagaa
    13921 ctttgccagc ggtatattca gtgcaggcat ggagtttctc ttcacctggg gagagctgct
    13981 ggggattatc ggcctgattg gtcatctgat gcgtgcccgg gctgaagggc gtcacagtat
    14041 gggaccggga aaatttatcg ccggtatcgt catctgcggc atgctggtcg ccctgccatc
    14101 cttcatcaat gccgggggta cccagatggg attcagggca gattccttcg gccctatcgc
    14161 ttatgtacaa ccaaccacct ttggtgccgc tgccggtgcc gccaatgcca tgctctcact
    14221 ggtaaaactg gccggagtgg ggtttgccat gaacggcatt tccatctggc gtaaatccct
    14281 gctggacggg cataccgccc tgagcagcag tgagtccatc agcaaaggaa acgtcaagtt
    14341 tgttgccggc gttctgctgg tttttatcga tcgcgtcatc gatgcctctc tggcgagtct
    14401 tggtctcgcc ttctgatcca cattcatttt cagtcattca gtctgttagt taaggagaca
    14461 ctatatgtca cgattccgtg ccatggcggc gcgtctgtgc gctgcattta tttatgttca
    14521 gttatgcctg gtggcctgcc gtgatcggct gatacgcctg ttacttctgc ttcccgtgat
    14581 actgctgccc cacacagcga tggcaaacac cctattcgat atgatcaaaa ccgttggcga
    14641 tggtgcaaaa ggctccagca gttcatttgt ggctatcggt caggttattg gcctggtgct
    14701 ctttatcggc ggcctgcttg gcctcaaaca aatcggtaaa aaacccggtg ccgggctggc
    14761 ctgcctgctg tcaattctgg tcggcctctg ccttgccgtc gtaccggaac tgatgggtag
    14821 cggacagaaa acgctcggtc tttctccggt ttcaatcagt taatccgcaa aggcaccggg
    14881 aaaccggtgc cgtcttcaca caatgaggag gaccagacca tgtcctgtga gctgccccct
    14941 ttcaccctgc tcagaacaga gcacggcaac gtgattgccc gcgacaaccg ggataatcag
    15001 gagtttgtgt ttgcagaatg ttattccacc gccatcgcag cactgattct ggctgccgtc
    15061 accctgtata cagagagaaa aacgtatgaa caaaacaccg ccactcatcg ccccggtccg
    15121 ggaacgctgg cttttcccgc tcccgcaacc ggacacggta ttcagcggtg aatccctgct
    15181 gccaccacca gtgctctcaa cacccggacg ctgtccctgc tgtcgcagga ccgtcactca
    15241 ccgcttcatt ctggaggatt catggccatt acagcagatg gctgacacct gccgggatac
    15301 ggtcgtcctg ctggaaaaaa acctcacccg ggtgatgcgc cagaaaaaac atccggtacc
    15361 ggaaaatgct gacgaaaaaa agaaacacac ccgaaccctg caggatgcgg agcggtccct
    15421 ggcacaggcc cgcctgtcgg ccaggcgact ggccctgcgt catgtggaaa agtcacaaat
    15481 cgtcacaaca gatgcactca gcgaaaatga aagtgaactg ctgcagccgg aaggcccgcc
    15541 ctttcatctc tgtgccttct gccacgcctg gcactgcctg aacggctacg ctgccgcaca
    15601 gggggtgatg gtctggctcc ctgatttgca tccggcctcg gtagttgctc tcaatgcacg
    15661 ggcactgaag gaaattttca gcgatgagcg taagcgagtc agacagggac gggccgtact
    15721 gaatgccctg gtacagaacc gcctggccgt tgaggagaaa tttcgcacct ggcgtccggc
    15781 ggattttgct gatgcactgc gccgctggcc gccggcacag cgtaaaacgt tgagggagaa
    15841 aatggacggg gtggcgctca ttctgctgcc ggattcgttt cctgacaaaa agtacgtcat
    15901 gtgacacaca ccggtaccga tgacattctg ccggcacaca ttcaacttac agaggaacaa
    15961 aaatggatac acactctgcc ggatatcagt tcactgcaga ttcattccgg cgttttatta
    16021 tacggacgct gactgacaat attccgcctg acggagatga aaaagaaatt gcccggttca
    16081 ttctccgcca cccggcattt aacggcggct ggctgctctt tctggctgca gaaattcatg
    16141 aaagatcccg tacccataaa agcatcaccg tcacagtgag cgtcgataac tatctctcat
    16201 ggatggatat tgccgtatcc ggtctcccgg aattaccgga tatgcccttc tgtgattttt
    16261 tcattcgccg ccagcagttt atctatccgg gtgtgccccc gacaaaatca ggagaagaat
    16321 gatgaaaatt caccgcatat ccccggagac gcttataaca ctcatccacg cacacctggc
    16381 cggaaaaact gacagtaccg ccaaagaaga gcaccggcta ctccgtcgtt tcctgcggga
    16441 tgatgacggc aggctggccg gcgtactgct gaatattgcc ggaatacttc agttcaacag
    16501 ggaattaagt gcgcggcata attatccggc cacacccctg actgaatttt cattaaggaa
    16561 acggggaaaa caacttcatc tctgtctctg cagtctccgt ttcttttata ttcctccggt
    16621 atttatacaa aacaaaagaa gaaaatctat tgttgtacat cttaataaga tcacttataa
    16681 acaaacccat tcaatacgct gacgaagtga ttcatactga ttatactcat ctttgcctat
    16741 atgtttttta agcgtattaa caatatcata ttgaggatgt cctgaattgt cagtatacag
    16801 tgttgttgca ggaaggactg cataatctga tatttcacaa ccagtataca gttctttgtc
    16861 agttcggcag gaatggcaaa gagaaagaca tgtatacagg tctgtgtttt ctgcctcctc
    16921 aggagataac cacgacacat attgcaaatc atctgtatca ggataacaat tttcttcctg
    16981 aacctgaact ctgaaaatat agaaaaataa ttcatctttt gaaaggcatg ctattttatt
    17041 cccccaatgc accgcattat caagttgatc cgataaataa atccatcctg gtgtcgtctc
    17101 taacgaccag catataacag aaggagcatc agttcttatt ttcccatctt ttattatttg
    17161 cagacctctg ctcattgttg tcgcatgaaa aagatttaat attttatttt cagccttcac
    17221 tctatttcct catttcccaa gaaacatatt aaaaacggaa aacattacac ataacctgga
    17281 ggacaaatca tgactttaaa tttccctgcc ggggaaacta cactattctc agcaaaaaac
    17341 atcctgacag caatactcac tctgcacaac ttacacgcat agcgttccca tttgaataca
    17401 gttgcagccc gcgatttatt ttgtcttccg gagccacctc tgccatgaat atcaaccgca
    17461 ttatttttgc cattgaagac tgtatcagta ccctgtcacg gtattccgtt tccgcattct
    17521 tccccgatta ctgtgatctg cacaccgtca tcggtctgga ccagcaggac cgcgaacgcc
    17581 gcccctggct gaaagccccc tacattgcca ccacaaaaca ggggtattac ctttccgtct
    17641 ttgaaatcag cggtgccctg aaagaaatgg atgaaaaccc ggacctgaca gcccccggca
    17701 cactggaatc actgattaca gcgctggcag agcgaatgaa cactgcctat aaaaacaccg
    17761 gtcacaaaat cagttttgtt ttcgaacgcg atccggagcg tggaagggat gaaatcgaag
    17821 ccatgctctt accccagcga aaatccatcg cacgcacggg gatcctgctt caggacgttc
    17881 tggatgaaaa agtcaccacg ctcaccccct ggcttgtccg ggaacgctgc tggctgaccg
    17941 tctggagctc tgaagaactg ctgagccgga ccgatcgcaa ggaccaccag acccgtatca
    18001 ggaaactcgc tgaacacgct ccgccagccc gatttgcgca ggatccatgg cgctggacac
    18061 tttccgcact caaaatacgg catgacgcct ttctggacac gctggagcag gccctgacac
    18121 atgattctga tggtctgctg gttcgcctga tggatattca tgaagtggga cgggaaatcc
    18181 gccgtcaact ggagcgcaac agcacacctg cagtatggca gcctcatctg cctgaagatg
    18241 cccgtcctgc cggctggcga cagggcgagg atacctctgt attccacgca ccgtcgctga
    18301 atctgcagtt attcaccaca cagcctgaaa cacacggcag cctgataaag gccggtgacc
    18361 tctggcacgg tatggtcgcc attaccctgc caccccagaa tctgcggaca ttcaaccagc
    18421 tggtcaggga tgtgccccac gcaatcccct ggcgtatccg gatggatctg atgcccggcg
    18481 gtatgaaggc gcttggggtc aagaaaaccc tgctgaccta cagcagcttt atttcccctc
    18541 tccggcccat gtatgactcg gtgatgatgc tgaatgacat caacaaacat gatccggtct
    18601 gtatcatgac cattgttgcc accacatggg gaaacagccg ggaagtctgt acccgcaacc
    18661 aggcgctcct gaaatctgcg ctggaaggct ggggagtatg cggcaccacc accaccttcg
    18721 gtgatccgag acgagcctgg gtgaacacgg ttctggcggc ctcccacggt tctggtccag
    18781 tcccccttta cccgcccctt tcacacgccc tttccctgtt tcccctgaac agagccggct
    18841 ctgtctggcg gggacaggga aatctgatga tgcacaccga ggacgggtct gcatgggagg
    18901 tcgcgctggc atcgtcacaa cagaacaagc acacggaact gacgcccggc gcacccggtc
    18961 tgggcaaatc ggtactcatc aacgcactgt cggagatcca gatagcctca gcccagaaga
    19021 acctgccttt cattgcctat atcgacaagg gattcagtgc acagggactg gtacagctta
    19081 tccgtgacag cctgccggag cagagaaagg acgaagccgt ggggatcatt ctgagcaatg
    19141 atccggaaca cacccgtaac ctgtttgatg tgatgtacgg tgctaaaaaa cccgccaccc
    19201 cggaaaagaa cttcatggta tctgtactct gcgcactgtg cgtggatacc ggcaccggtc
    19261 agccctgtaa tcccggtgac acccggcaaa tcataagcaa cctggtggat ctggccttca
    19321 gggagtatgg cgaaaacaac cccagactgt accgtgcagg aacagagccc cttgtggatc
    19381 tcgccctgga agagagtggc atcgctgaac aacatgacgc aggatggtgg aatgctgcca
    19441 cctggtttga aatcagggac atgctgcata tggctggcaa tatcccagcc gcccagcggg
    19501 cacactacca ggccatgccc ctgctggctg aaatgtccgc cctgctggga cagccatcca
    19561 tccgggatgt gttcggcaca gttcagcgcg ataactcagc agaattgtta ctggactaca
    19621 tccgccgtgc cctcgatcag ggacacagtg actacccgat gatgtccggc tgtacccggt
    19681 ttatgctcag ccccgacacc cgcgttgtgg ccgttgatct gaacaatgtg gccggtgaca
    19741 agacacctgc agggcgtctg cgcaccggca tcatgtacct gctggcagga caaatcgccg
    19801 gcggggattt tgtcctgccg cagtatcagg aggaaatacg gaaaaatctc gacccacgct
    19861 accatgaggt gattttccgc cgcattgaac agcttgatca ggaggtcaag accaaagtct
    19921 atgacgaact gcacaatgca aaagggatca atttcatctg ggaagccctg gacacccagg
    19981 agctggagca gcgtaagttc gggatacgca cagtgctgag cacccagcta ctgcaggact
    20041 atccaccggc agttctcaaa tccgccaaca ccctctggtt gctgcgttac cgccctgacg
    20101 aaattccgtt tctgcgggat aattttggtg tccctgaagt caccctgcgg cgcttcctga
    20161 aaatgccgga aggggctgca ccggatggca gcggtgtccc ggtactggct gtgttccgtg
    20221 taaaaaacgg tacgctggcc cggatcctga aattcactct cggtccactg gagctgtggg
    20281 cactgaattc ctcacccaaa gacagcgccc tgagacgtgc gctgacgcag gagctcgggt
    20341 cactgcgggc acgccagatc ctggcagaaa actttccccg tggttcagcc acctcactga
    20401 tagaacaccg cgcccgtaca catgacagtg aaaacgtcat ccatgatctg gctgcagaac
    20461 tcatccgtaa gcagggatat cacctctgat gtacagcata cacatcactc cccgctgccg
    20521 gcgtcctgca cggggacagg tcttcctgaa aacgctgcgc gtggacggtg acagtttcag
    20581 cttcaccctg ccgtccgcct gcttcacgct gcgtccctcc cccaatcccc gggtcatttt
    20641 cgccagtgac agacgggctg acggggaaat gagtggcgtt cgcctggctc tgcccggaga
    20701 gacaggcaca ctgataacaa cggccacctg gcaggtcaac gtgccggagc ggcctccttt
    20761 tgatatacag catgtcattc actggctcct gccctcgcct ctcacggcgc tgatgtccga
    20821 caacacctgg ctgtggccga atagccccgg cacaccggca ctgacggcgg gagactggcc
    20881 ggtaatggaa accgataccc cgacgctgaa acgcgtgaag cgtcgtacct tcacgctcat
    20941 cgataaccgc aaccgtcagg gatggatcac ccgtcgcttc cagtgcttcc cgctggctcc
    21001 gctgccggaa tcacacagtc accgcgtacc agacatcctg tatcccctga aaacggagaa
    21061 aaaagaatgc aaagacaacc agcaaccaca ctgcttgcac tcctggtagg ctgtgcggtt
    21121 atcccgtcca cccacgccta ttctgtgacc gtagtggcca gccagcctgt caccacacaa
    21181 atcatgccgg agctggccgc cattcagggc gtgatggcag agatggtccg tatgcagaca
    21241 gccaccggca cagccatcaa ccagagttca gagaaactgg cctctgtcat tgcgcaggat
    21301 gggcaggcca cccgtcagca gatgatattc agtaatgaaa cccgccggct tgaagaagcc
    21361 cggaagagct tcagcgtgcc ggactccatc tgcagtgaat ccgcctccgg agttgcagct
    21421 gaaagtcgcc gtgcagcagc gtctgcggct gcacgtctga gtcagggagg aggcgtcagc
    21481 agtaaaccca tccgtgaacg tctctcccgg gcagcagact caccaacccg cgaggcatat
    21541 gacagtgcag gcattcatgc cggttactgc acggaagctg aatatgcccg ctttggcgga
    21601 accgatgtct gccctgccgt gggcgagctg ccgggcggag acagccaggt gcgatcgctc
    21661 tatcagggtg caggcacagc agatacccct gccgccctca cctgggatca gaaacagatt
    21721 gatgctgcca cggcttacat gaaaaacacc gccagaccgt cagcgggtcg tgccccgggg
    21781 aaaggtgaag tgggaacaca gaccgggcgc acatatgtgg gcctgcagaa tgaatacaac
    21841 ggcatcatcg atgccgcttc tcatccgcaa ctgtcactga tagctgacag tacccctaat
    21901 gaggccaccc gtggagcgct gactgaagcc ctgcagtcac catccgctgc ggcatatttt
    21961 gaccggaccg catcatcaga ggcccgcacc cgtggtcaca tgagccagcg ggagtttgag
    22021 gcctttgaag ctggcagacg ctatgcaaac accgactggc agcaggatct gcagggcatg
    22081 gaaggcgata accttctccg tgaactgctg cgcaccaccg ccctgctgaa ctggcagatg
    22141 aacgacctga aagagcagat acgccagggt aatgttatcg ccgggcagca gctggcactg
    22201 gcggcacgac agtattacgg acagcgtctc ggtgaactgt cacaggccat gagccagggg
    22261 agcgtaagat gaaagacgac aacagcaccg acaaaaaaaa accggccccc cgtctccgcc
    22321 gcatcggtga catcgggata aacctgttcg caccactggc agaaacccgg tggatgctgc
    22381 gcagctgccg ccatatgctg ggccagcata aaacgcgcct gaagcgcctc atgcccgacc
    22441 ggcaaccgga gagtccggtc tgtatgacct gggaagaagc cgtcagggcc agcggcctta
    22501 cggtcaacga actggatcgg cgtctgcgac gtcgcaggat gttctggcgg acaggattct
    22561 ggctgatgat gataccggta cttctgctgt gcggcatggc ccttgctgcc acagcgcttc
    22621 cggccatcac cctgatacgt atactggcct gcctgttact gatgagtgcc attgcagcac
    22681 tgtgtgcctc aaaaaccctg attgtgtcat accgcctctg gcaactgaat gaacggcggg
    22741 tcagcgagga cgaacagggc accttccccg attacctgca ggcccggcac ggctggaaac
    22801 aggccacatg gctggccatt acccaccggc agtactgacc gtccggtctg caattccttt
    22861 tcctcattcc ggaggctgat gccctgcatc ccttcggttc cggagttatc gtgaaaatca
    22921 tacccagagc actgagtgcg ggtctggcgc tggctgccgt acctgcacag gcattcgtca
    22981 cctatcagga cattgtgagc gccgccacca atccggatga cctgtcgcgc caggcactgg
    23041 tcaccatttt tggcgacgtg gtcacaaatc ccctgacagc cagcagcaac accattatcg
    23101 gcaacctgtt catggtgttt aacgcaatta tcgccattat ggggatagtc tggttcctgt
    23161 ttattggaat ccgccatgtt gtccgcaccg gccaccaggg caatgtcttt aacaacgggc
    23221 gggacgtcgt cggaatgctc agtattgtga gcggttttat gatgattgtg cccaccgctt
    23281 ccggctggag tctggcacaa ctggtcatgc tctggggcgc atccatcatg ggtattggtt
    23341 ccgccaatat catggttcag acggctgcag aaaacatcgc tgaaggctat tcaatgaccg
    23401 tacagccagt tcatgtctcc acccgcacgg cagcacgggg aatactggag atggcgctgt
    23461 gcaaacacgc catgaatgca ggcctcgatg actttaaccg tactgcccgt tcatcaacgc
    23521 cccccatgag cgaatcctca cggacagaga acggcaggta taccgtcacc gtttcaaatg
    23581 gcagtgccac ctgcgggagt gtgagtctca ccgtgaaagg gaatggcagc aacaagcagt
    23641 ccgcgctgga ctattttttc aatcccttca ttgaaacgga gtatcaggga gtcctgacgg
    23701 cacaacggtc tgccatggat accatgctcc gtgacctgga cagcgcagca caggaatttg
    23761 tgtcagcgta ccggacccga cgcgacacgg gtaacggcaa cctgccggat attgaaagcc
    23821 gtattcagaa ggctgccaac cggtatgaag aaacggtgca gaaggcgttg ccaacagata
    23881 acgcagaaac tgagcgcagg gagcagctga aatcctacct gaccacctac ggctgggttg
    23941 ccctgggggc ctggtaccag acctttgcca ccgccaacca gcgactggca gaactggccg
    24001 accgtgcccc cactgtgagt ggagtttcat cgcagggcga agccggagat actgagttcc
    24061 tgaaggaagt caaaggagct taccaggcac agttacagaa ctcaccatat actcctgcgc
    24121 tgggaaatct aacatcttct gatgagatat atgctgccaa tacaaatgat ccacaaagtg
    24181 caattataaa gttaatgtca ccggacttta cgaaactgac atataaaatc gcaactgtat
    24241 attctagtac aggaactaca tcctatcagg caaatcccct gctgaagatg aaggatatcg
    24301 gtgattacac catggtcaca acagaaacca tctgggcggg ttataccacc gtcagggtgc
    24361 tttctactgc cggcagcaac tccgtttttg gccgactggt caatgccgtc agcggcttat
    24421 ctgatgcagc aaaggctttt ctggaagcct ccgcaccacc actctacttc ctgctgtttt
    24481 tgctgttctg tgccggtttt tcgttatcta tttacctgcc gtttatcccg tttatcttct
    24541 ggatgaccgg catcggcaac tggatagtca gcgtactgat tggatgtgct gctggccccc
    24601 tgtgggccgc cacccatctg ggaacaagtc aggaccgggg aagccgggcc gcttacggtt
    24661 acatctacct gatagacagc atgatcagac cgctgctgat ggtgttcgga ttcttttttg
    24721 catcggtcgc catcgtggcc ggcggcacaa tcctgaatgc gctgtttgct gctgcgctga
    24781 tgaatgtcca gataaattct gtgacgggcg tgttcagcat ggcgggattt ttgatgattt
    24841 atgcccgagc ctgtaccacg ctggttgcgg ccatttttgc acttcaggct tatctgcctg
    24901 accatgtaat taattttctg ggcgggcgtg acggtgttaa tatgctgggg aatatggttt
    24961 cttccgtcaa agatattatt gccggcggaa acaccaatat acgcagaaca cctggcgttc
    25021 gtgaagagaa actaaaaaat attaataggg atgataagga tggaataaaa caatagagta
    25081 aaaacatcaa aaacaaataa aaagcattgc catattcgcc tggtgatatt aaaatacaac
    25141 caggtgaata tggaggttta ttatgaatga acaaagaagc agatatcctt cctggtgggc
    25201 atattattac cttgtccgca acgcttattt tttatttgga gttccatttt taattttttg
    25261 tgccattgct tttacagc
//
LOCUS       DAZFXT010000066        24539 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.066, whole genome shotgun sequence.
ACCESSION   DAZFXT010000066 DAZFXT010000000
VERSION     DAZFXT010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.066"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            135..1340
                     /gene="gltS"
                     /locus_tag="ACHKW0_002872"
     CDS             135..1340
                     /gene="gltS"
                     /locus_tag="ACHKW0_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015501 - glutamate:sodium symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015813 - L-glutamate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HFO7475674.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            1343..2209
                     /locus_tag="ACHKW0_002873"
     CDS             1343..2209
                     /locus_tag="ACHKW0_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475675.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISEWDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(2194..4275)
                     /gene="recG"
                     /locus_tag="ACHKW0_002874"
     CDS             complement(2194..4275)
                     /gene="recG"
                     /locus_tag="ACHKW0_002874"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HFO7475676.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKQTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(4281..4970)
                     /gene="trmH"
                     /locus_tag="ACHKW0_002875"
     CDS             complement(4281..4970)
                     /gene="trmH"
                     /locus_tag="ACHKW0_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0141100 - tRNA
                     (guanine(18)-2'-O)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HFO7475677.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(4977..7085)
                     /gene="spoT"
                     /locus_tag="ACHKW0_002876"
     CDS             complement(4977..7085)
                     /gene="spoT"
                     /locus_tag="ACHKW0_002876"
                     /EC_number="2.7.6.5"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HFO7475678.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(7104..7379)
                     /gene="rpoZ"
                     /locus_tag="ACHKW0_002877"
     CDS             complement(7104..7379)
                     /gene="rpoZ"
                     /locus_tag="ACHKW0_002877"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HFO7475679.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(7434..8057)
                     /gene="gmk"
                     /locus_tag="ACHKW0_002878"
     CDS             complement(7434..8057)
                     /gene="gmk"
                     /locus_tag="ACHKW0_002878"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HFO7475680.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            8315..9997
                     /gene="ligB"
                     /locus_tag="ACHKW0_002879"
     CDS             8315..9997
                     /gene="ligB"
                     /locus_tag="ACHKW0_002879"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HFO7475681.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHRFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVTEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(9994..10611)
                     /locus_tag="ACHKW0_002880"
     CDS             complement(9994..10611)
                     /locus_tag="ACHKW0_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HFO7475682.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(10903..11727)
                     /gene="dinD"
                     /locus_tag="ACHKW0_002881"
                     /pseudo
     CDS             complement(10903..11727)
                     /gene="dinD"
                     /locus_tag="ACHKW0_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
     gene            complement(11948..12811)
                     /locus_tag="ACHKW0_002882"
     CDS             complement(11948..12811)
                     /locus_tag="ACHKW0_002882"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HFO7475683.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            12938..13654
                     /gene="rph"
                     /locus_tag="ACHKW0_002883"
     CDS             12938..13654
                     /gene="rph"
                     /locus_tag="ACHKW0_002883"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HFO7475684.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            13720..14361
                     /gene="pyrE"
                     /locus_tag="ACHKW0_002884"
     CDS             13720..14361
                     /gene="pyrE"
                     /locus_tag="ACHKW0_002884"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HFO7475685.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(14398..14994)
                     /gene="slmA"
                     /locus_tag="ACHKW0_002885"
     CDS             complement(14398..14994)
                     /gene="slmA"
                     /locus_tag="ACHKW0_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0010974 - negative regulation of division
                     septum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HFO7475686.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(15101..15559)
                     /gene="dut"
                     /locus_tag="ACHKW0_002886"
     CDS             complement(15101..15559)
                     /gene="dut"
                     /locus_tag="ACHKW0_002886"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HFO7475687.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRVCLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(15537..16757)
                     /gene="coaBC"
                     /locus_tag="ACHKW0_002887"
     CDS             complement(15537..16757)
                     /gene="coaBC"
                     /locus_tag="ACHKW0_002887"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HFO7475688.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARIAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGMLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            16929..17597
                     /gene="radC"
                     /locus_tag="ACHKW0_002888"
     CDS             16929..17597
                     /gene="radC"
                     /locus_tag="ACHKW0_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7475689.1"
                     /translation="MKNNSQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            17814..18050
                     /gene="rpmB"
                     /locus_tag="ACHKW0_002889"
     CDS             17814..18050
                     /gene="rpmB"
                     /locus_tag="ACHKW0_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HFO7475690.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            18071..18238
                     /gene="rpmG"
                     /locus_tag="ACHKW0_002890"
     CDS             18071..18238
                     /gene="rpmG"
                     /locus_tag="ACHKW0_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HFO7475691.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            18336..19145
                     /gene="mutM"
                     /locus_tag="ACHKW0_002891"
     CDS             18336..19145
                     /gene="mutM"
                     /locus_tag="ACHKW0_002891"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HFO7475692.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVELVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(19184..19663)
                     /gene="coaD"
                     /locus_tag="ACHKW0_002892"
     CDS             complement(19184..19663)
                     /gene="coaD"
                     /locus_tag="ACHKW0_002892"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HFO7475693.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(19671..20948)
                     /gene="waaA"
                     /locus_tag="ACHKW0_002893"
     CDS             complement(19671..20948)
                     /gene="waaA"
                     /locus_tag="ACHKW0_002893"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HFO7475694.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            21361..22419
                     /gene="rfaQ"
                     /locus_tag="ACHKW0_002894"
     CDS             21361..22419
                     /gene="rfaQ"
                     /locus_tag="ACHKW0_002894"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HFO7475695.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            22416..23540
                     /locus_tag="ACHKW0_002895"
     CDS             22416..23540
                     /locus_tag="ACHKW0_002895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7475696.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            23533..24330
                     /gene="rfaP"
                     /locus_tag="ACHKW0_002896"
     CDS             23533..24330
                     /gene="rfaP"
                     /locus_tag="ACHKW0_002896"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HFO7475697.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         5764 a         6048 c         6598 g         6129 t
ORIGIN      
        1 gccatttgtt cattaacagc tgagaatttg acccaggaaa gcataactcg atacccgtca
       61 tagttcatga agttgccact gcgcgatgaa gtatgacgag tatgaaagag tgatgcggat
      121 acaaaggagt aactatgttt catctcgata ctttagcaac gcttgttgcc gcaacgctga
      181 cgttgctgct cgggcgtaag ttggtccatt ccgtctcctt tttgaagaaa tacaccatac
      241 cggaacctgt tgcgggtggt ttgttggtgg cgctggcgct actagtactg aaaaaaagca
      301 tgggctggga agtcaacttt gatatgtccc tgcgcgatcc gttaatgctg gctttcttcg
      361 ccaccattgg cctgaacgcc aacattgcca gtttgcgtgc cggtgggcgt gtggttggca
      421 tcttcttgat tgtggttgtt ggtctgttgg tgatgcaaaa tgccattggc attggtatgg
      481 ctagcctgtt agggcttgat ccgctgatgg ggctgttggc cggttctatt acgctttccg
      541 gcggtcacgg tacgggcgct gcgtggagta aattgttcat tgaacgttat ggcttcacca
      601 atgcgacgga agtggcgatg gcctgtgcaa cgttcggtct ggtgctgggc ggcttgattg
      661 gcggtccggt ggcgcgctat ctggtgaaac actccaccac gccgaacggt attccggatg
      721 accaggaagt cccgacggcg tttgaaaagc cggatgtggg acgcatgatc acctcgctgg
      781 tgctgattga aactatcgcg ctgattgcta tctgcctgac ggtggggaaa attgttgcgc
      841 aacttttggc tggcactgct tttgaactgc cgaccttcgt ctgtgtactg tttgttggcg
      901 tgattctgag caacggtctg tcaatgatgg gcttttaccg cgtctttgag cgtgcggtat
      961 ccgtgctggg taacgtaagc ctgtcgttgt tcctggcgat ggcgctgatg gggctgaaac
     1021 tgtgggagct ggcttcgctg gcgctgccga tgctggcgat tctggtggta cagaccatct
     1081 tcatggcgtt gtatgccatc ttcgttacct ggcgcatgat gggcaaaaac tacgatgcgg
     1141 cagtgctggc tgcgggtcac tgtggttttg gcctcggtgc aacgccaacg gcaatcgcca
     1201 acatgcaggc gatcactgaa cgctttggcc cgtcgcacat ggcgtttttg gtggtgccga
     1261 tggtcggtgc gttctttatc gatatcgtca atgcgctggt gattaagctg tatttaatgt
     1321 tgccgatttt tgccggttaa aaatgaaaaa gcgtcggaag gaaccggaaa cgctacgtga
     1381 acactgtcgc catatttttg gtgatgagcc gccagtgctc tgtgtatggg aaacggaatt
     1441 tgattacgca gatgccgaac tcaaggcgct ggcggctaaa gagtggcagc agatcagcga
     1501 atgggatttg agtgcttact atgtactcaa tctcgtttat aacgaaccga tgcaaataga
     1561 gttgtttcgc tatctgtttc cgctctgcct ggcgcaatgg catgaaaccg ttttagctgg
     1621 cggttatggt gatcacttcg aagaatcctt aatgaaggca ttgtgtcggc cctatctctg
     1681 gcaggagatg atgaatgctt cccaacgcca gcaggtacgg caatttttac tggacaccgc
     1741 gttgcagcgt atggataacg agcgtggttt caataacgtt ctttgctggc tggcggtgtt
     1801 caatactctg ggtggggccg cgccgttaat tcgttctctc tggtcgcgct ggtgggcgct
     1861 ggatacgcca ggaaaagcgg tgtgtgctat tcagtatgcc gcgcatctaa tttatcccat
     1921 agaagccaat ccgttatggt cgcaagaatg gataggctgg gggcatccat taggccataa
     1981 agatggctgg tcctctgata atcgcgcttt tcttcgccaa atgttaacac ctgaaatgat
     2041 tgtggctggc gtacaggcag cggctgaaat tctgcgtggt gaaccggaag gcgcaatggc
     2101 ggcgcgaatt gcacaggatg cgtatgaggc aatggatatc ctcactatcc agattgaaga
     2161 tttactgcgt gacttgtctt gtgatgagtc tggtcatgcg ttggagtaac gttccgtctc
     2221 cggcatccag cgttctatca gggcttttgc ttgttgtggg taacgttcgt gaatatgtcg
     2281 cgccaggcgc tgaacttccg ggatcatcgc ctgatcgcgc agtaaatccg ccactttaaa
     2341 ctcagcatta cccgtctgac gcgtacctaa cagttcaccc ggtccgcgaa tctctaagtc
     2401 tttctgtgca atcacaaaac cgtcgttgct gtcgcgcagc acttgcagcc gaatttgcgc
     2461 ggttttggag agcggcgttt tgtagagcag cacgcagtga gaagccaccg caccacgacc
     2521 tacacgcccg cgaagctggt gtaactgcgc cagacccaga cgctccgggt tttcgataat
     2581 catcagactg gcgttaggca catcaacgcc gacttcaata acggttgtgg caaccagcag
     2641 gtgtagctca ccttgtttaa acgacgccat caccgcctgt ttctcggcag gtttcatccg
     2701 cccgtgtacc aggccaacgt tcaactctgg tagcgccagt ttcaactctt cccaggtagc
     2761 ttccgccgcc tgcgcttcca gcaattccga ctcttcaatc aacgtacaaa cccagtatgc
     2821 ctgacgacct tcagttatgc aggcgtggcg cacgcggtca atgatgtcgg tacggcgggt
     2881 atcaggaata gcgaccgtag tcactggcgt gcggcctggc ggcagctcat ctatcaccga
     2941 ggtatcgaga tcggcatacg cagtcattgc cagcgtgcgg gggatcggcg tggcggtcat
     3001 gatcaactga tgcggatgga agccctgctg ctggcctttc tcccacaatg ccagacgctg
     3061 atgtacgcca aaacgatgct gttcgtcgat aatcaccagc gccaggccgt taaactgcac
     3121 ctgttcctgg aagatggcgt gtgtaccgac aatcatctgc acctgaccgc tggcgatggc
     3181 ttcctgctgt gccagccgtg ctttaccttt ctgcttaccg gcgagccagc ccacttcgat
     3241 accgagcggt gcaaaccagt tgcggaagtt attggcgtgc tgctcggcga gtaattcggt
     3301 tggtgccatc aatgctacct gtttgccgtg ggcaatcgca cgcaacgcgg cgagggcggc
     3361 gaccagcgtt ttaccggaac ctacatcgcc ctgcaccaga cgcatcatcg gcacatccag
     3421 cgccatatcg cgctcgatct ccgccactac gcgtgcctgt gcgcccgttt gcttgaacgg
     3481 taaggcggcg aggagtttat ttttcagcgt gtcattggcg ctcagcggct gggcatgaaa
     3541 acgctgtgct ccggcacgta aggctaacat gctgaggttg tgcgccagca gttcttccag
     3601 aatcagacga cgttgcgccg gatgctgccc ggtttccaga tcgctaagct gtagcgtcgg
     3661 tggcgggcgg tgcaaagtgc gcaacgcttc cggtagcgtc atcattcctt gtgacagttc
     3721 cggcggcagg agttcttcaa tggcgcaggt gtcgagcaga tccagcgcct ggtcggttaa
     3781 tttacgcagc gtggcctgct ttacgccttc cgttgttgga taaaccggcg tgagcgtttc
     3841 ctgtaattct ggcgtgctga gatcgccctg cacgcggtat tccgggtgga tcatctccgc
     3901 accatattta ccgcgctttg cttcgccata agccagtaca cggcggcccg tcgccaggct
     3961 atttttcatt gccgcgctga aattgaaaaa gcgcatggtg aggatgccgg aaccgtcgct
     4021 gatctggcag gtcatcatcc gccgaccgcc gaaggagata ttgcagttca gcacttcgcc
     4081 ttccaccgtg gcataaacgc ccggcagtag ttctccgatg gggtagagat gggtgcgatc
     4141 ttcgtagcgc aggggaaggt gtaagagtag atcctgcacg gtatgcaggt tgattttcgc
     4201 cagtttgtta ctaagtgctg cgccaacgcc cgttagggaa ctgagtggga cagcatctaa
     4261 caggcgacct ttcatggcac ttaccctgca gcctgcatag tagcccacca gtcggcatca
     4321 gcttcgatct cgccttgctg attgacgtgg ggataaggca ggcctttgcg ttttgcgact
     4381 ttcgccagca ccggatagcc gccttcaaac aacaggcgtt gttgctctgc ttccggcaac
     4441 atgctgtttt cacgcaggta catgcctgca ttttgccgct gacgctgggc ttcgtaaaga
     4501 atgagggctg aggcaacgga aacattcagc gactgcacca tgccgatcat cggaatgatg
     4561 atgtcctgat ccgccagggc caatgcttcc tgcgtgatgc ccgttttctc ctgtcccatc
     4621 aaaatgcagg tcgggcgagt gtaatcaatt tcgcggaaat cgacagcgtt atcagaaaga
     4681 tgggttgcca gaatctgcat gccctggcct ttgagatgag cgacggcatc gccaatggtg
     4741 cggtgtgttt tcacctgtac ccagctgtta ctacccgccg ctgccgaagc catggtgcgc
     4801 atgcggctac caggccagac ggcgtgaact tcatgtacgc caacggcatc tgcggtacga
     4861 ataatcgcag aaacgttatg aggtttgtgg acctgctcca tgcagacggt cagatcaggc
     4921 tgccgcctgg cgagcatttc gcagatgcgt gcataacgtg ttgggttcat aaaacattaa
     4981 tttcggtttc gggtgacttt aatcacgtct ggcatcacgc ggattttgcg catgatattc
     5041 gccagatgca cacggtcacg ggcggtcaga cgaataaagg cgctgtagac gcgaccatct
     5101 ttctcttccg tattcaaact ttgaatattc gaagtcgtgg tgttaattgc cgccgtcagg
     5161 tttgccagcg cgccctgatg attgaacatc tccaccttga tttcggtgat gaactcctgc
     5221 gccgtctctt tatcccattc cacagccata aacttctctg gctctttctg gtagccacgg
     5281 atattacggc aggattcatg gtggatcacc agacctttac cggggctgac gtgggcgata
     5341 atcgggtcgc caggaatagg gcggcagcat ttcgcaaagg tgatcagcac gccatcggca
     5401 cctttaatgg gcagatgtcc gtggctttgg gttgccggtg gaatggaggc gtccccatgt
     5461 tgcagatttt tcgcgaccac cacgctcatt gcgttaccaa gtccgatttc tgccagcaga
     5521 tcgtcaagcg ttgccagctt catgcgatcc agctcgcgct gaatattttc ctgcgggatt
     5581 tcattgagct tacggctacc acccaaagca tggttgagca gacgacggcc caggcttaca
     5641 gaatcatcac gcttgaggtt tttcagcaac tgacgaattt tggcgcgcgc tttcgagcta
     5701 acgacaaagt tcagccaagc ggcattcggg cgagcgcccg gagcggtaat gatttcaacg
     5761 gtctgaccgc tagtaagcgg ctgcgacagc gggtaaggct ggcggtcgac gcgtgcgccc
     5821 acgcaggcat ggccgatatc ggtatgcact gcataagcga agtcgacggg cgttgcaccg
     5881 gcaggcagct cgacaatgcg cccttccggt gtgaaaacgt aaatctcatc cgggaagaga
     5941 tcggatttaa cgctctcgat aaattcaaac gaactaccgg cgctctgttg cagctccagc
     6001 aggctttgca tccagcgctg ggcgcggatt tgtgcggtag tactggtttc gccgtgctct
     6061 ttataagccc agtgcgcggc aacacccatc tccgccatct gatccatatc ttcggtacgg
     6121 atctggacct caaccggcac gccgtgcggg ccaatcatcg aggtgtgcaa cgactgatag
     6181 ccgttcgctt taggaatggc gatatagtct ttcacgcgac ccggacgcgg cttgtacagg
     6241 ctgtgcatct ggcccagcac gcgataacag gtgtcagaat cattgacgat cacgcggaaa
     6301 gcgtagatgt ccatgattga gtgaaaacgc tgctctttga gcaccatttt gcagtaaatc
     6361 gaataaagat gcttttcgcg accactgacg cggcacggta ttcccgcttc ctgcaaacgc
     6421 ccttcgattt cagagaggat tttttggatc atctctttac ggttgccgcg cgcggctttc
     6481 accacttctt taattacgcg gtaacgattg ggatacagcg cctcaaaacc cagctcttcg
     6541 agttcggttt taatgtggtg gatacctaaa cggtgcgcca gcgggctata aatttcgaga
     6601 gtttcacggg cgatgcggcg acgtttgtcc gggcgaagtg agcccagcgt gcgcatgttg
     6661 tgggtacggt cggcaagttt gatgaggatg acgcggatat cctgcaccat cgccataatc
     6721 atcttgcgaa agttttcggc ctgcgcctct ttcttatcgc ggaacttgag tttatcaagt
     6781 ttcgacaccc cctctaccag ctcggcgacg cttttaccaa aaagctgttc catatcctgg
     6841 taggtggcgg gagtatcttc aatcacgtca tgcagcagcg ccgccatcag cgtttcatag
     6901 tcgagtttca tctcggccag aatgcaggca accgctaccg ggtgcgtgat atagggttca
     6961 ccgcttgaac gtgtttgccc ctcgtgagca tcacgtgcaa cgagatacgc ctgccgcaga
     7021 cgcttgattt ggtcttccgg caggtaggtt tgaatcagtt gattcaggct ttcaaacaga
     7081 tacaagggcg acccgctttg tgattaacga cgaccttcag caatagcggt aacggcttgt
     7141 aattcagcgg cttcctgctc ttgctgttcc tggcgttcgc gaacgtcgag gatctggttg
     7201 ttgatcagac cttcttcgat ttcgcgcagc gcgattacag tggttttatc gttttcttcc
     7261 ggtaccagcg gatcctttcc gcctacctgc atctgacgag cgcgacgcgc ggcgaccagt
     7321 accaggtcaa aacggttacc aattttctct acagcgtcct gaacagttac gcgtgccata
     7381 cttaaaaagc tccacaggtg aagaaatgac tgggcatgat actgaaatca ggttcagtct
     7441 gccaacaatt tgctgattaa agcgtcatga cgctgctttt ggcggctcat gcgcagacgt
     7501 tcggcgcgaa taatggtctt caaatcggtc aacgcggtat cgaagtcatc attcacaatc
     7561 agataatcat attcggcgta atggctcatt tctgcaacag cttgcgccat acgctttgca
     7621 atgacctctt cgctgtcctg accgcgaccg cgtagacggc ggtccagttc aattttggac
     7681 ggcggtaaaa taaagatact ccgcgcgtgc ggcatcttct ggcgaatttg ctgcgcgccc
     7741 tgccagtcga tatcgagaaa aacatcgaca ccggtcgcca gtacttgctc aatggcctca
     7801 cgcgaagtgc catagtaatt accaaaaact tctgcgtgtt cgaggaacgc atctctgcta
     7861 atcatttctt taaattcatc atgattaaca aagaaataat gttcaccgtg gacttcacca
     7921 ggacgcggtt ggcgtgtggt gtgtgaaaca gaaacctggg tgtcatacaa cggttgggtt
     7981 tttaataaag cctgaatcag gctggattta cccgcgccac tgggggcaga aacaatataa
     8041 agcgtgcctt gagccatgag tatctttcgt atgtgattag cgaaataaag cctacatacg
     8101 agcttattat acacggcgcg gccatgtgac gtagcccttg ctcacacttt ttgcgcaaag
     8161 ttgttgcgtt ttgcgtagct gtttccgcat ttgcctgtga tttactgcaa taaagacgat
     8221 tctcaggaag cgtcctcgca gactccgttt ttgccactcg gctttgcctt caccacgttg
     8281 tatatctgcc agcctgacta cagcggaggt gaagatgaaa gtatggatgg cgatattaat
     8341 aagtatcttg tgctggcaat catctgtgtg ggcggtctgt ccggcctggt cgccagccag
     8401 agcacaggaa gaaatttcac gcctgcaaca gcaaataaaa cagtgggacg atgactactg
     8461 gaaggaagga aaaagtgagg tggaagacgg tgtttacgat cagttaagcg cccgtcttac
     8521 gcagtggcaa cgctgctttg tgagcgagcc ccgcgacgtg atgatgccgc ctttaaatgg
     8581 tgcggttatg catccggttg cccatacggg tgtacgcaaa atggcggata aaaacgcatt
     8641 aagtctgtgg atgcgagaac gtagcgatct ttgggtgcag ccaaaagttg atggcgttgc
     8701 ggtaaccctg gtttatcggg acgggaaact gaacaaagca atcagtcgcg gtaatggcct
     8761 gaaaggcgag gactggacgc agaaagttag cttaatttcc gctgtgccgc aaaccgttag
     8821 cgggccttta gccaacagta cgcttcaggg ggaaatattt ctccagcgcg aggggcatat
     8881 ccaacaacaa atggggggaa taaatgcccg cgcaaaagtt gctggcctga tgatgcgcca
     8941 ggacgatagc gacacgctga attctctggg cgtttttgtc tgggcatggc cggatggacc
     9001 gcagttaatg accgatcgtt taaaggagct ggctaccgca ggttttactc taacgcagag
     9061 gtatacccgt gcggttaaaa atgctgatga ggttgcgcgc gtacgcaatg agtggtggaa
     9121 agcgaaatta cccttcgtca ccgatggcgt ggttgtgcga ggggcgaaag aacccgaatc
     9181 ccgccattgg ctaccaggcc aggcagaatg gctggtggcc tggaaatatc aacctgtagc
     9241 tcaggttgcc gaagtgaagg cgattcagtt tgcggtgggt aagagcggta aaatatcggt
     9301 ggttgcgtca ctcgcacctg tcatgctgga tgataaaaaa gtccagcggg tgaatattgg
     9361 ttccgtcagg cgctggcagg agtgggatat tgcgcctggt gatcagatcc tcgtcagcct
     9421 tgccggtcag ggtattcctc gcattgatga tgtggtctgg cgcggtgcag aacgtacaaa
     9481 accgacacca ccagaaaacc gctttaactc gttgacctgc tactttgctt ctgatgtttg
     9541 tcaggaacag ttcatttcac gcttagtctg gctggggtca aaacaggttc tggggctgga
     9601 tggtattggt gaggccggct ggcgcgcgct gcatcagact catcgctttg agcatatctt
     9661 ttcctggctt ttattaacgc cagagcaatt acagaacacg ccggggatcg cgaaaagtaa
     9721 aagtgcgcag ctatggcatc ggtttaatct ggctcgtaag cagcctttta ctcgctgggt
     9781 gatggcaatg ggaataccgc taacccgggc ggcgcttaat gccagtgatg aacggtcctg
     9841 gtcgcaactt ttatttagca cggagcagtt ctggcagcag ctgccgggga ctggatcggg
     9901 gcgcgccaga caggttactg aatggaagga aaatgcgcaa atcaagaagc tgggcagttg
     9961 gctggctgcc cagcagatca caggttttga accttagtgt ccttcgtggc tgtagtaaaa
    10021 gaccggtaat cccagcttaa ggcgtagcgc cagtaagcgg gcaaaaaagc cgaataccag
    10081 ggtggagata attaccacat catggttaga aacatagtgt tgcagcgcga tgtacagcac
    10141 ggcggaggca aatgagacac cggcatacaa ctctttctgg aataccagtg ggatacgttt
    10201 acagaacata tcgcgcaaaa cgccgccaaa cacgccggtg gttaccgccg cgacaacggc
    10261 aataattggg ccgtgcccca tatccagtgc aacctgtgcg ccgatgatag aaaagaccac
    10321 cagtccaagc gcatcgagca ccagaaacac tttgcgcagg taaggcatta caggagcaac
    10381 gatagtggtg agtactgcgg cggtagcgac gataatcaca tattccgggt gtttgaccca
    10441 gccgagcgga tagtggccca gcagaatatc gcgcactgac cctccgccaa ttgcggtggc
    10501 ggtcgcaata ataattacgc caaatgtatc catgcggcgt cgcccggccg ccagagcccc
    10561 cgtcatggct tcggcagtaa tgccaaccaa atacaaaatg tgtaacagca tggttctctc
    10621 cctaaaaatc tgtctggcag ggtagcgatt tgtgcgtatt gtcacgattg agatttttta
    10681 aggtgagagg aaattgataa cttgtagtta tgtgaaatcg ctattttctg tagcgtagat
    10741 gcattcttct gacttcttgt ctgagaaaat gcataagaaa aaataggtta aaattgaggg
    10801 gtggaaaaga aaggtgcaat gtttagccat tgtgttcacc gcacctggac tttggcttac
    10861 gcatcgggta tctcaatcaa caaaaagagt ttttagtttc tgctatttct tctctgtaat
    10921 ttttacactg ttttccaact gcttgatgct gacctgcggg gtcggcaact cctcaggcat
    10981 ggttccgcca agttcctgaa tggtttgcct caccttgcta cccacgtcaa agtgtgtggt
    11041 attagcttgc tgttttgaat tcacgccatc ccgcttgagt ttttcttctg tttgggtagc
    11101 tcgaaagaga ttagccgcca gttctgttga acccatatga tccaggatct tctgactctt
    11161 tttcagcccc ttcagttggt ggatggcttt ctgatctaat ccaccataca gcccctggta
    11221 accatgattc tgaaatatgg cgaaatcagt agctgtcgct accccagctt actgtgctgc
    11281 ctcaaccaat tgtttgttat gttctttcaa ctcatttctc agaaacagac gcttttcgtc
    11341 ttcacgaagt tgcttaaatg cttcatcgtc agcaagctcc tgccgtcggg tctggatagc
    11401 aaaataagtc tgccctgccg caatgaccgg tttcgcaggg tcgccgtttt gtactaccag
    11461 gtaacaggca tagcgggaga gatgaacatc ttcaagttct cgttgagccc ctgaacctaa
    11521 tacaaccatt ttggtggttt caacgaaatg gtcagatgca gcttgattgc tggcttcgca
    11581 cgcttgagtc gcgcgggcca gtaccttttg aaaattgcgc cagtcccgat aatccagtaa
    11641 cggtgccaat tctctagctg accaaaattc ttgcccttca gtgccataat gccgtatttc
    11701 ttcaaaaggt tgatgatgtt cgttcattgc ccactccatg tgatatcgat tgcaaagaaa
    11761 gtaaatctgt aactgtattt atatacagtt gtgagtatct cacgttctca gtgtgctggg
    11821 caaaaaaaca tcgcaaaatc tggagcaggc acgcaaaaca gaattgaagt tgcggggaag
    11881 aaaaatgccg ctctgtttga gtgggcggca tttgtgaagg ctgaaaagta agaattggcg
    11941 ttacgagtta ttcaatgttc tgaatctgct cgcgcatctg ctcaatcaac actttcagct
    12001 caatcgcgga gtttgtcact tcggcattga tagacttcga cgcaagagtg ttcgactcgc
    12061 ggttgaattc ctgcatcata aagtccagac gacgaccaac ggcttctttt ttcttcagaa
    12121 tgttgtaggt ctctttgaca tgcgcttcca ggcggtccag ttcttcggca acgtcaattc
    12181 gttgtgccag cagaaccagc tcctgctcca gacggttgtt ttccaactga acctgagcat
    12241 cttccagctt cgcgaccaga cgctcacgct gccattgcag gatttccggc atatgggcgc
    12301 ggactttgac cacttcggcg gtgacgcctt ccagacgctg ctcgatcaat gctttcagtg
    12361 cctgaccttc ggtttcgcgc gcgacaataa agtcgtccag cgtaccatcc agcgccgcga
    12421 gaatttcagc ggcaatggcg tcaagatcct gctcctgggc tgccatcacg cccggccagc
    12481 gtagaatatc aaccgggttg atttcccctt cgtcactctg catttttacc cagttcgcgg
    12541 cagttaccag ctgtttagcc agtttttcgt tgaggatcag ctcaccttgc gcgctaacat
    12601 ctggctcata gcgcagggta cattccactt taccgcgcgt caggcgagaa cgaatacgct
    12661 cgcgaacgac aggttcaagg ctacggaact gctccggcag acgaaagtaa gtttccagat
    12721 aacgctggtt taccgagcgc atttcccagg ttgcgctccc ccattcaccc ttgatttcac
    12781 gccgggcgta ggcggtcata ctgcggatca tagacgttcc tgtttataaa aggagaggtg
    12841 gaaggattat agccatcgat gccttgtcag gataggaata accgccggaa gtccgtataa
    12901 tgcgcagcca catttgtttc aagccggaga tttcaatatg cgtccagcag gccgtagcaa
    12961 taatcaggtg cgtcccgtta ccctgactcg taactataca aaacatgcag aaggctcggt
    13021 gctggtcgaa tttggcgata ccaaagtgtt gtgtaccgcc tctattgaag aaggcgtgcc
    13081 gcgcttcctg aaaggtcagg gccagggctg gatcaccgca gagtacggca tgctgccacg
    13141 ttctacccac acccgtaacg ctcgtgaagc ggcgaaaggt aagcagggtg gacgcacaat
    13201 ggaaatccag cgtctgatcg cccgtgctct tcgcgcggca gtagatttga aagcgctggg
    13261 tgagttcacc attacgctgg actgcgacgt gcttcaggct gatggtggca cgcgtaccgc
    13321 gtcgattacg ggtgcctgcg tggcgctggc agatgcgcta cagaagctgg tggaaaacgg
    13381 caagctgaaa accaatccga tgaaagggat ggtagccgca gtttctgtcg gaattgtgaa
    13441 cggcgaagcg gtttgcgatc tggaatacgt tgaagactct gccgcagaga ccgacatgaa
    13501 cgtagtgatg accgaagacg ggcgcatcat tgaagtgcag gggacggcag aaggcgagcc
    13561 gttcacccat gaagagctac tcaccttgtt ggctctggcc cgagggggaa tcgaatccat
    13621 tgtagcgacg cagaaggcgg cgctggcaaa ctgattttta aggcgactga tgagtcgcct
    13681 tttttttgtc tgtagaaaag taagatgagg agcgaaggca tgaaaccata tcagcgccag
    13741 tttattgaat ttgcgcttgg caagcaggtg ttaaagtttg gcgagtttac gttgaagtcc
    13801 gggcgcaaaa gcccctattt cttcaacgcc gggctgttta ataccgggcg cgatctggca
    13861 ctgttaggcc gtttttacgc tgaagcgttg gtggattccg gccttgagtt cgatctgctg
    13921 tttggccctg cttacaaagg gatcccgatt gccaccacta ccgccgtggc gctggcggag
    13981 catcatgacc ttgacctgcc gtactgcttt aaccgcaaag aggcaaaaga ccacggtgaa
    14041 ggcggcaatc tggttggtag cgcgttacaa ggacgcgtaa tgctggtaga tgatgtgatc
    14101 accgccggaa cggcgattcg cgaatcgatg gagattattc aggctaatgg cgcgacgctt
    14161 gctggcgtgt tgatttcgct tgatcgtcag gaacgggggc gcggcgagat ttcggcgatt
    14221 caggaagttg agcgtgatta caactgcaaa gtgatctcta tcatcaccct gaaagacctg
    14281 attgcttacc tggaagagaa gccggaaatg gcggaacatc tggcggcggt taaggcctat
    14341 cgcgaagagt ttggcgttta aagaaactcg ccggatgaaa agtcatccgg cgtcatatta
    14401 ctgcaactgt gccgcaatta gcggccagcg ggcgtcaaaa tcatccgtcg ggcggtattt
    14461 aaattcgctg cggacaaaac gtgacagcat accttcacag aaggccagga tctggcttgc
    14521 cagcagggtt tcatcggtgg tgtaaccttc accctcacgc attctctttt cacgcaatac
    14581 ctgacgcagc tgcgcttcaa tacgctcgaa cagctggttg atgcgccctt gcaggcgatc
    14641 ctgttcaaac attagcgcat gaccagtgag gatgcgggtc aggccaggat tacgctcacc
    14701 aaaaccgaga agcagcaaca caatcagacg caggcgcgct gtggtgtctt tctcatcttt
    14761 cagaatcaga ttgatgcgag taatcaggct atcttcgata aactcaatca ggctatcgaa
    14821 catgcgggtc ttactgggga agtggcgata cagtgccgct tcggaaacgc cgacagaggc
    14881 ggccagtttt gccgtcgtga tacgttggct tccatcgctg gattccagca tcagcgccag
    14941 agactgaagt atttcctcgc gacggttcct tttcgcagtt tgtttttctg ccatgttaca
    15001 aaatacccct gaaaataagc acttgccagg cggcacccac gctatgaccg caaacgaaat
    15061 gtttgcggct atgttatgac gttattcgga tgcgtatgtg ttactgacga ccagagtgac
    15121 caaagccgcc ttcaccgcgg tcggtggcgt cgaaatcttc caccagatta aattcagcct
    15181 gtactaccgg aacaaaaatc atctgggcga tgcgttcgcc aggttgaatg gtgaagctgt
    15241 cctgaccacg gttccacacg gaaatcatca actggccctg atagtcggaa tcgatcaatc
    15301 ctaccaggtt accaagcacg ataccgtgct tatgtcccaa tccggagcgc ggcagcatca
    15361 ttgccgccag tgaaggatcg gcaatatgaa tcgccagccc ggtcggaacc agcgtagtgt
    15421 cacccggagc cagttctacg gcgtcgtcga gacagacacg caggtcaagt ccggcagagc
    15481 cagaggtggc ataagtcggg agcggaaatt ccttcccaac gcgcgggtcc agaatcttaa
    15541 cgtcgatttt tttcatcata acgggtcacg atctcgtcga gtaataattg gccaaggagc
    15601 tctttgcgct caagcggtaa gactttatct ccgtcctgcc agaaaaggtg taatgcgttg
    15661 ttgtcgctgt taaatccttg agttggctgg gaaacatcgt tcgcgcagat cagatcaagg
    15721 tttttacgga tacgtttttg ccgggcgtat tcttccacat tatttgtttc ggcggcaaat
    15781 ccaacgacgt agggtcgatg gtcttttagt gcggcaacgc ctgcgacgat atcggggttt
    15841 ttaaccattt ttattgttaa ttcatcaccc tgcgtggcct gctttttgat tttctctggg
    15901 gccacggtag ctgcgcgata atccgccacg gcggcgcaac cgataaaaat attttgctgc
    15961 tgtacagaag cattcacggc ggcttccatt tccagcgcag tcatcacatc aacacgttta
    16021 acaaacggtg gcgtcggtag tgaaaccgga cctgatacca gcgtgacgtt cgcgccacga
    16081 cgggcagcgg cggcggcgat agcaaaaccc atcttgccgg agctgtgatt agagatataa
    16141 cgcaccggat cgagcggttc acgcgtcggg ccggcggtaa tcataatgtt cagatgtttc
    16201 aggtcgttga cgggcgaaaa atgcgccacc gccatatcca caatggttaa cggatcgagc
    16261 attcgcccag gaccgatatc accacaagcc tgactgccac tgtctggccc ccagatgagc
    16321 ataccacggg aagcaagcac ctctaaatta tgctgcgtgg cagcggcacg gtacatctgc
    16381 tggttcatgg cggggagcac ggctacaggc gcaggtgtag ccagacaaat cgtcgatacc
    16441 aggtcattcg ccattccggc agcaatacgg gcaatcaaat ctgccgtggc aggggcgaga
    16501 atcactaaat cagcccattt acccagctca atatggccca tagcggcttc ggctgccggg
    16561 tccagcagac tgtcggaaac gggataacca gaaaccgcct gcaagctaag tggggtgata
    16621 aaggcttttg ccgcttcggt catggctacg cggacgtcgg ccccgcgatc gcgcaaacga
    16681 cgcaccagtt caggggtttt ataggcagca ataccgccgc taacgccgag aacgattttt
    16741 ttaccggcca ggctcatgat gatttttcct gtagggtgac accagaagtt ggcgatttta
    16801 tcacaatact tttgttgtcg tgccttcacc tgagattcac tttgcgaggc gctttccagg
    16861 attgaaaact ggccgtcgat ttaacgaaac ggctatgaca ggatgcgagc accacaaagg
    16921 aggtgaaggt gaaaaacaat tcacagctgt tgatgccgcg cgaaaaaatg ctgaagtttg
    16981 gtattagcgc cttaacggat gtcgagctgc tggcgctatt tctgcgtacc ggaacgcgcg
    17041 gtaaagatgt attaaccctg gcaaaagaga tgctggagaa tttcggctct ctttatggct
    17101 tgttaacctc tgaatatgaa caatttagtg gcgttcatgg aattggcgtg gcgaaatttg
    17161 cccagttaaa ggggattgct gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag
    17221 aaagcccttt actcagcccg gagatgacga gggaattttt acaaagccag ctcacgggtg
    17281 aggagcggga gatctttatg gtgatctttc tcgactccca acaccgggtt ataacgcata
    17341 gccgtctttt ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa attatccgcg
    17401 aagcgataaa aataaacgcc tcggcgctga tccttgcaca taatcaccct tcgggttgtg
    17461 ctgaacccag taaagcggat aaactcatta ctgaacggat aataaagagt tgtcagttca
    17521 tggatttacg cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt tcttttgccg
    17581 aacgcggctg gatttaaccc gctatgcgcg atccttcggg atctttgtct gttcgggact
    17641 tgagcacatc gctgagtcag cgtatactac gccacctttg agaatctcgg gtttggcatt
    17701 tgggcctggc aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa agcctgacga
    17761 ggcgccaata ccccatacga agctcgagct aatttgattt ttggagaata gacatgtccc
    17821 gagtctgcca agttactggc aagcgtccgg tgaccggtaa caaccgttcc cacgcactga
    17881 acgcgactaa acgccgtttc ctgccgaacc tgcactctca ccgtttctgg gttgagagcg
    17941 agaagcgttt tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag
    18001 gcatcgatac agttctggct gaactgcgtg cccgtggcga aaagtactaa gtacttagag
    18061 gaaataaatc atggctaaag gtattcgtga gaaaatcaag ctggtttctt ctgctggtac
    18121 tggtcacttc tataccacta cgaagaacaa acgtactaag ccggaaaaac tggaactgaa
    18181 aaaattcgat ccagttgttc gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt
    18241 ctcgctttga tgtaacaaaa aacctcgctc cggcggggtt ttttgttatc tgcttgcccc
    18301 catattgact gcatctgttc attcctggag atgctatgcc tgaattaccc gaagttgaaa
    18361 ccagccgccg cggcatagaa ccgcatctcg ttggtgcaac cattcttcat gcagtggtgc
    18421 gcaacggacg cttgcgctgg ccggtttcag aagagatcta ccgtttaagc gaccaaccag
    18481 tgcttagcgt gcagcggcgg gctaaatatc tgctgctgga gctgcctgag ggctggatta
    18541 tcattcattt agggatgtct ggcagcctgc gcatccttcc agaagaactt ccccctgaaa
    18601 agcatgacca tgtggaattg gtgatgagca acggcaaagt gctgcgctac accgatccgc
    18661 gccgctttgg tgcctggctg tggaccaaag agctggaagg gcataatgtg ctgacccatc
    18721 ttggaccgga gccgcttagc gacgatttca atggtgagta tctgcatcag aagtgcgcga
    18781 agaaaaaaac ggcgattaaa ccgtggctga tggataacaa gctggtggta ggggtaggga
    18841 atatctatgc cagcgaatca ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat
    18901 cactgtcgct ggcagagtgt gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt
    18961 cgattgagca gggtggtaca acgctgaaag attttctgca aagtgatggt aaaccgggct
    19021 atttcgctca ggaattgcag gtttacgggc gaaaaggcga gccgtgtcgg gtgtgcggta
    19081 cgccgattgt ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga
    19141 agtaattcat gcgcgccgga tggcatacca tccggcataa acgctacgct aacttcgcca
    19201 tcagcgcctg atggacattc tccggcagga aatgggtgac atcgccctga tggcgcgcca
    19261 cctctttcac caacgatgaa gagataaacg accactcttt cgacggcatc agaaacacac
    19321 tttccagttc cggcattaag tggcgattca tatgcgccag ctgcatttca tattcaaaat
    19381 ccgccaccgc acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg
    19441 ccatcaaatc actaaacccg accacttcca cgttccccag atgcgcggtt gcctgctgtg
    19501 ccagcgccac acgctcttcc agggtaaaca tcggtttttt actggggctg gcggcaatcg
    19561 ccagaataac gtgatcgaac atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg
    19621 taatgggatc gaaagtaccc ggataaatcg cccgtttttg cataacaacc tcaatgcgtt
    19681 ttcggtggca ggtaaggttc cagcagttga agcagacgct gtagcgcgcc ctggttttga
    19741 tacagtactt caacggcatg acggccatag aaactacggt aatcggcgtc ggtgagtaaa
    19801 gaggacacct cttttgcaag cgtagtggca tcggtaacgg taatcagccc gcttgcctgc
    19861 tccagccgcg cgcaaatgtc tttaaagtta aaagtatgcg gccccatcaa taccggaata
    19921 gcgtgtgcgg cagcttccag cggattatgc ccaccacgtt caaccagtga accgccaaca
    19981 aaggcgagat cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc gccaactaca
    20041 acctgcgtgc tggtggaggg gacttcccct gaagagcgtg tgatatagct tagtccagcc
    20101 tggcggacaa ggttaatcgc atccgggaag cgttctggat gacggggtac caggatgagc
    20161 aataaattcg ggaattgctg taacaatgcc tgatgtgcgg caatgaccac gctctcttca
    20221 ccttcgtgag tgctggtggc aatccatacc gggcggtgtg gtgcccattg gcggcgtagc
    20281 gtcacggctt tagcagccaa ctgcggcgtt acagaaatat cgaatttcag gctaccggta
    20341 acggttacct ggttattttt tgcgcccagc gccacaaaac gtgcaccatc ttcttcattt
    20401 tgcgcagcaa tcagcgtaat acgacgcagc aagcggcgga cgaatttacc cagtttggca
    20461 taacctgcgg ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg aattttacgt
    20521 ttatgtagcg ccgcaatcag gttaggccat agttcggttt ccataatcaa caccagttta
    20581 gggtcgactt tattcaggaa acggttgagt gcatcgggca ggtcatacgg cagataaacg
    20641 tgctgaacat ccttcccgaa agccgattgt acgcgctccg aaccggttgg cgtcatggtt
    20701 gtaacggtaa tcggtaaatc aggataacga tgacgcagcg cgcgcaccaa cgggatcgcc
    20761 gccagagttt caccgacgga gacggagtgc agcataatgc cgcctggttt tagcggatgg
    20821 cggtaaaaac cgtaacgttc accccagcgt tttcgatagg ccggagcctt acgtccgcgc
    20881 acccagagcc gtatccagat cagcggctga ataaggtaga gaagggcggt gtaaagcaat
    20941 tcgagcatag taaatagctg acttatggat gtgctgggga ttctatgtat ttagctgtgg
    21001 ctttaccatt acttttcccg tttttgactt aaatagcaat ttacccgcca attcttgctg
    21061 aacatctata ctttttttgt atttttatgc gattcattga tttctgctgt acgtgcagga
    21121 aggaatcgca gagcaacagt gaaagaatat ggttgctaat gattttattt gattgatttc
    21181 tttattgaaa atcaacaatg tgcttaaaaa tcattcgggt cggattgccg cgaaagaagt
    21241 gatacactag cgcgtcaata tcagtgcggt atcagtattc aggtagctgt tgagcctggg
    21301 gcggtagcgt gctttttctg cttcacctta atcggataat ctcaacaaaa agagttactt
    21361 gtggataagc catttcgaag aattttgctc attaagatgc gttttcatgg ggatatgtta
    21421 ttaactactc ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg
    21481 ctgctttatc aggacaccat cccgatcctg tctgaaaatc cagagattaa cgcgctctac
    21541 ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt tcatctcatc
    21601 aaggtattac gtgccaataa gtatgacctt atcgtcaatc ttaccgatca atggatggtt
    21661 gctatactgg ttcgcttatt aaatgctcgt gtgaaaattt cccaggatta tcatcatcgg
    21721 cagtctgctt tttggcgtaa aagtttcacc catttggtgc cgttgcaggg tggaaatgtg
    21781 gtggaaagta acttatccgt gctgacacca ttgggacttg attcgttggt gaagcagaca
    21841 accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt
    21901 gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa atgctgggac
    21961 aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga agtcgttctg
    22021 acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca gggatgccag
    22081 acgccaccag taacggcgct ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc
    22141 gatcatgcgc agctgtttat tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt
    22201 aatacgccgc tgatatcgct gtttggtgcg acagatcata ttttctggcg tccctggtca
    22261 aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt
    22321 gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc tgctgtcgat
    22381 aaattactgc cctcctccac gacaggtacg tcgttatgat cgttgctttt tgtttatata
    22441 aatattttcc ctttggcggt ttgcagcgtg attttatgcg tattgctcag acagtcgccg
    22501 cccgaggtca tcatgttcgg gtttataccc agtcgtggga aggcgaatgc cctgatgtat
    22561 ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat gcggagtatt
    22621 ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca
    22681 aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag aaagtagcgc
    22741 aggaaaaagg ctttttctat cgcctgacgt cacgttatcg ccattatgcc gcctttgagc
    22801 gggcaacctt cgaacagggt aagccgacac agttgctgat gctgacagat aagcaaatcg
    22861 ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg ccaccgggga
    22921 tttatcctga tcgtaaatat agccagcagc cagccaatag ccgtgaaatc ttccgtaaga
    22981 agaatggaat aaccgaacaa caatatttat tgttgcaggt cggttcagac ttcacgcgta
    23041 aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg cgccacaaca
    23101 cattgctata tgttgttggg caggataaac cgcgaaaatt tgaggcgctg gcagaaaaac
    23161 gcggcgtgcg cagtaatgtt cactttttct cggggcgcaa tgatgtctcg gaattaatgg
    23221 cggcggcgga tttattactg catcctgcct accaggaagc ggcgggaatt gtgctgctgg
    23281 aagcgattac tgcaggatta ccggtactaa caacagccgt ttgtggctat gcgcattata
    23341 ttgtcgacgc taattgcggc gaggctattg ctgagccatt ccgccaggaa acattgaatg
    23401 agattttacg caaagcgtta acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc
    23461 gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg gatatcataa
    23521 cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg cggcaaagat
    23581 ccttttgagg aagttaaaac cttgcagggt gaggtatttc gtgaactgga aactcgccgt
    23641 actctgcgct ttgaaatggc gggcaaaagc tattttctca aatggcatcg cggcacgacc
    23701 ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg cgctgaccgc
    23761 gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta tggggtggca
    23821 tttggcgaaa aaggcatgaa tccgctgacc agaacttcat ttattattac cgaagatctg
    23881 acaccaacca taagtcttga agattacagt gctgactggg cgactaaccc tccagatgtt
    23941 cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgcg
    24001 ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc tttttccggt
    24061 aaggaagagg agttaaaaat ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc
    24121 gttccacgtc gttggcggga taaagatctt attgggcttt atttttcttc gatgaatatc
    24181 ggcctgactc agcgggatat ctggcggttt atgaaagtgt attttgccgc cccgcttaaa
    24241 gacattctca agcaggaaca aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc
    24301 agggaaagaa cgattcgaaa atcgttgtaa cccgtaactt atttttgcca aaattttgga
    24361 tacagaataa atatgtctca actcaatgat agtgacatca tcctttttga gtataatttt
    24421 cattatcaaa atataagatc taaaaatact cttgatatag catttggtat tgacagaaat
    24481 tttctttttg gatgtggtgt agccatcgca tctattctat taaacaatag agaaatctc
//
LOCUS       DAZFXT010000067        24404 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.067, whole genome shotgun sequence.
ACCESSION   DAZFXT010000067 DAZFXT010000000
VERSION     DAZFXT010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24404
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.067"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(172..1053)
                     /gene="prmA"
                     /locus_tag="ACHKW0_002897"
     CDS             complement(172..1053)
                     /gene="prmA"
                     /locus_tag="ACHKW0_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HFO7475698.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(1065..2516)
                     /gene="panF"
                     /locus_tag="ACHKW0_002898"
     CDS             complement(1065..2516)
                     /gene="panF"
                     /locus_tag="ACHKW0_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015233 - pantothenate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015887 - pantothenate transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HFO7475699.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(2506..2748)
                     /locus_tag="ACHKW0_002899"
     CDS             complement(2506..2748)
                     /locus_tag="ACHKW0_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HFO7475700.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(2857..4206)
                     /gene="accC"
                     /locus_tag="ACHKW0_002900"
     CDS             complement(2857..4206)
                     /gene="accC"
                     /locus_tag="ACHKW0_002900"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HFO7475701.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(4217..4687)
                     /gene="accB"
                     /locus_tag="ACHKW0_002901"
     CDS             complement(4217..4687)
                     /gene="accB"
                     /locus_tag="ACHKW0_002901"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HFO7475702.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(5665..6639)
                     /locus_tag="ACHKW0_002902"
     CDS             complement(5665..6639)
                     /locus_tag="ACHKW0_002902"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HFO7475703.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            6791..8731
                     /gene="csrD"
                     /locus_tag="ACHKW0_002903"
     CDS             6791..8731
                     /gene="csrD"
                     /locus_tag="ACHKW0_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HFO7475704.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            9036..10079
                     /gene="mreB"
                     /locus_tag="ACHKW0_002904"
     CDS             9036..10079
                     /gene="mreB"
                     /locus_tag="ACHKW0_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HFO7475705.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            10145..11248
                     /gene="mreC"
                     /locus_tag="ACHKW0_002905"
     CDS             10145..11248
                     /gene="mreC"
                     /locus_tag="ACHKW0_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HFO7475706.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPVNTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            11248..11736
                     /gene="mreD"
                     /locus_tag="ACHKW0_002906"
     CDS             11248..11736
                     /gene="mreD"
                     /locus_tag="ACHKW0_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HFO7475707.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            11745..12338
                     /gene="yhdE"
                     /locus_tag="ACHKW0_002907"
     CDS             11745..12338
                     /gene="yhdE"
                     /locus_tag="ACHKW0_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HFO7475708.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            12328..13797
                     /gene="rng"
                     /locus_tag="ACHKW0_002908"
     CDS             12328..13797
                     /gene="rng"
                     /locus_tag="ACHKW0_002908"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HFO7475709.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            13865..17665
                     /gene="yhdP"
                     /locus_tag="ACHKW0_002909"
     CDS             13865..17665
                     /gene="yhdP"
                     /locus_tag="ACHKW0_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HFO7475710.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKET
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADNTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            17821..19266
                     /gene="tldD"
                     /locus_tag="ACHKW0_002910"
     CDS             17821..19266
                     /gene="tldD"
                     /locus_tag="ACHKW0_002910"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HFO7475711.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(19394..20323)
                     /gene="aaeR"
                     /locus_tag="ACHKW0_002911"
     CDS             complement(19394..20323)
                     /gene="aaeR"
                     /locus_tag="ACHKW0_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HFO7475712.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            20506..20709
                     /gene="aaeX"
                     /locus_tag="ACHKW0_002912"
     CDS             20506..20709
                     /gene="aaeX"
                     /locus_tag="ACHKW0_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump operon protein
                     AaeX"
                     /protein_id="HFO7475713.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            20717..21649
                     /gene="aaeA"
                     /locus_tag="ACHKW0_002913"
     CDS             20717..21649
                     /gene="aaeA"
                     /locus_tag="ACHKW0_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HFO7475714.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            21655..23622
                     /gene="aaeB"
                     /locus_tag="ACHKW0_002914"
     CDS             21655..23622
                     /gene="aaeB"
                     /locus_tag="ACHKW0_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HFO7475715.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            23714..23986
                     /locus_tag="ACHKW0_002915"
     CDS             23714..23986
                     /locus_tag="ACHKW0_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HFO7475716.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(24041..24304)
                     /gene="yhcN"
                     /locus_tag="ACHKW0_002916"
     CDS             complement(24041..24304)
                     /gene="yhcN"
                     /locus_tag="ACHKW0_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HFO7475717.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         5798 a         6152 c         6605 g         5849 t
ORIGIN      
        1 ttagaaaata gtgactaaaa tttacactca aagaaatttg agtaagttct caatttttct
       61 ttatgaatga aaattttggc acgcaaattt gcgtaaataa ccggcaaatt tacgtgcctt
      121 ttgtgagctt gctcgcactt cgccccgcgt caccctacgg cgatgcgaag gttaattctt
      181 acgaccggta atacggcacc actcttcttt ttccacgacc gggtccagtg cgaagctatc
      241 ggcataagct tcacaaacgc tctctgcctg gcttgccaga ataccggaaa ggcccagcaa
      301 accgcctgaa accggcagga cgctgattaa cggtgccagt tcacgtaatg ggcctgcaag
      361 gatgttagcg accaccacgt cggctttcat ttcttctggc tgatctttcg gtaagtagag
      421 ttccagacgg tcagaaacgc cattacgttc ggcgttgtcg cggctggcct gaatcgcctg
      481 cggatcgata tcaataccaa tggcttttgc tgcacccagt ttcagcgccg cgatcgccag
      541 aatgccggaa ccacagccaa agtcgatgac tgttttaccg gttaaatcga ggctgtcgag
      601 ccattgcagg cacagagagg tggttggatg ggtacccgta ccaaacgcca gccctggatc
      661 taacatcacg ttgacggcgt tttcgtccgg cacatcacgc cagctagggc agatccacag
      721 tcgttcacca aagcgcatcg ggtggaaatt atccatccat tcgcgctccc agtctttatc
      781 ttctagttgt tcgattttat gcgcgaagcc tgcgccgagc agcggatggt tttccagaat
      841 cgccaccacg tcgttcatat cggtttcagc gtcgaacaga ccaatcacat cggtgtcgcc
      901 ccacaggcgc gtttcgcccg gcagcggctc aaatactggc gtatcgtggg tatcctgaaa
      961 agtgatagaa acggcacccg cttccatcag cgcatcgcta agatcttccg cgttcgcgcc
     1021 ggtggtgttc agtttcagtt ggatccaagg catggcaaaa ctctttattt atcagtagtc
     1081 aaaacggtag cttgcgggac ggatgtaccg aaacggtttc cgaccaggaa agccagcaaa
     1141 cttagtagta acgagggcac aatagggtgg aagcccaggt actgaatatt cagcgtcgcg
     1201 agtacggcat acagcacgcc gccaacgatc atcgcactta gcgcgccttt ggcgttggcg
     1261 cgttcccagt aaagacccag caccagcggc cacaggaaaa cggcttccag cccaccgaag
     1321 gccagcaaat tcagccagat gatcatttct ggcggcttcc aggcggcaag cagcagcaac
     1381 gcgccgagaa ctaacgtaat taccgccgac atccgcttca gacgcgtctc gttttgcatt
     1441 tgatccggac ggatattcag atagagatct ttaatgatcg tagcggaact ttgcagcaac
     1501 tgggcattga tcgtcgacat gatcgcagcc ataggtgcag ccaggaagat tccggcagca
     1561 aacggtggca gcacttttac cattaacgtc gggatcacca gatccggtac ggtgagatcg
     1621 gggatcactg cacgacctaa cgctccggcc aggtgcatac cgaacatcag gattgcgact
     1681 acaatcgtac cgatgatgat cccccggtgt actgctttgc tgtctttata agagatacag
     1741 cgcaccgcag tatgcggcag gccaatcacg ccaaagcaca ccagtaccca gaacgacgtc
     1801 ataaaggcag gcgacagaat atcgtcagcg ccttgtggcg taaccagttg cggatcgatg
     1861 gtttgcaagg tctgtactgc gttgcttaag ccgccagcgg catgtaccac gccaataagc
     1921 agcacaacgg tgccaatcag catcacaagc ccttgcatgg tgtcgttcag cacgctggcg
     1981 cgaaagccac caaaggcggt atataacgca atgctgatac caaaaatcag cagcccggtt
     2041 tcataaggaa tacccgccgc ggtttccagc aggcgcgcac cgccgataaa ctgcacggtc
     2101 atcgcaccaa cgaacgcaac cagcaaactc aaactcgcca gccacaccag aagacgactc
     2161 tggtagcggg caaacagcat atcgttcagc gtcactgcat tgtagcggcg cgcaagaatc
     2221 gcaaacttct tgccgagaat accgagtgaa agccagactg caggaagctg aatcatcgcc
     2281 agcaataccc agcccagccc gtatttataa gcagctcctg gcccgccgat aaacgaactg
     2341 gcactgatat aggtcgcggt gagcgtcatc gccagcacaa taccgcccat agagcggctg
     2401 ccgaggaaat actcattaag gaaggtgccg gtgctccgtt tacgcatcgc ataaaccgag
     2461 ataccgaaca ccaccaccag ataggcgacc agcggtagaa ttacttcaag ctgcatcgtc
     2521 atcctccagt gggatatcgc gatagataaa tttcaccatc gcccagcaca gtccaataaa
     2581 cagtagcggc gtcaggatgc aggccatctc aaaccagcgc ggaaagccgg taaaaccggg
     2641 ggcaacgcca ggtaagtaag cggctactaa ccaaactgcc agatacaaaa gggtcagccc
     2701 cagcgcccag cgcgcctctt tatgggcctg aacaaaacga gtgtccattt tttgtccctg
     2761 atgggtgaag aaagcgagga ttgtacctta tgggggttgt cgatccccag taataaaaaa
     2821 ggccggaaaa tccggccttt tgacgctttt gcagtcttat ttttcctgaa gaccgagttt
     2881 tttctccaga tagtggatgt tagtgccacc atgctggaag ttctcgtcat tcatgatgcg
     2941 gatctgcaga tcaacgttgg ttttgatacc gtcgatgatc agctcctgca gcgcattctt
     3001 catgcgagca atcgccacgt cacggttttc accgtagcaa atcagcttac cgatcattga
     3061 gtcatagtac ggcggtacgg tgtagcccgc gtagatatga gactcccaac gcactccaaa
     3121 accgccaggt gcgtggaaac gggtgatttt gcctgggctt ggcaggaagg tgttcggatc
     3181 ttcggcgttg atacgacatt ccaccgcatg gccgcgaacg tgaacttctt cttgcttgat
     3241 cgacagcggt tgaccggcag cgatacgcag ctgttctttg atcaggtcaa cgccggtgat
     3301 catttctgta accgggtgtt ctacctgaat acgggtgttc atttcgatga aatagaactc
     3361 gccgttttcg aacaggaact cgaaagtacc tgcaccgcga tagccgatat caacacacgc
     3421 tttagcacaa cgttcgccga tgtagcgacg cagttccggt gtaattcccg gtgctggtgc
     3481 ttcttcgacc actttctggt gacgacgctg catagagcag tcacgttccg ccagatagat
     3541 agcgttgccc tgaccgtcag ccagtacctg aatctcgacg tggcgaggat tttccaggta
     3601 tttctccatg taaaccatat cgttattgaa tgcggctttc gcttccgcac gggtcatgga
     3661 gatggattgt gccagttcag cgtcgccgcg cactacacgc ataccacgac cgccgccgcc
     3721 gccggaggct ttgataatca ccggataacc tatgcgttta gcaatggcac ggtttttatc
     3781 catatcgtcg cccagcgggc cgtcagaacc tggtacgcaa ggaacgcccg cttttttcat
     3841 cgccgcgatt gccgacactt tgtcacccat caggcgaata gtgtctgctt tcgggccaat
     3901 gaagataaag ccggagcgtt caacctgctc ggcaaagttg gcgttctcgg agaggaagcc
     3961 gtaacccgga tggattgcta ctgcgccggt gatttcagcg gcgcttatga ttgccgggat
     4021 gttcagataa ctttttactg acggagcagg gccaatacag accgtttcat ctgccagtaa
     4081 tacgtgtttt agatcgcgat ccgcgctgga gtgcacagcg acagtcttga tgcccagttc
     4141 tttacaggca cgaagaatac gcaatgcaat ctcgccgcgg ttggcaataa caattttatc
     4201 cagcatgttc gcctcgttac tcgatgacga ccagcggctc gtcaaattct accggttgtc
     4261 cactttcgac cagaattgct ttcacggtac cggatttgtc cgcttcgatc tggttcatca
     4321 ttttcatggc ttcaacgatg cacagggtat cgcccacgtt gactttctga cccacttcga
     4381 tgaacgcttt tgcgtccggg cttggggtgc ggtagaaagt accaaccatc ggggaacgta
     4441 cgatgtgacc actgatttcc gctgctgctg gcgcttccat ggaaggaacg gtcgccggag
     4501 cggctgcgtt agattgagct ggctgctgca tcattggtgc agcgtaagct tgttgcatca
     4561 cagggaaact tgcggcagga gctgcacggc tgatgcgtac tgactcttcg ccttcggaga
     4621 tttccagttc ggagatgcct gattcttcaa ccagctcgat cagtttttta atcttacgaa
     4681 tatccatgag tgggttccgt actctttgtt tagtgtgatt gtgacaggcg tttcaccgcc
     4741 gtctgtaaag cgtatgcccc gacagctgta tgcatagcga taaattccag caggccggga
     4801 cgccggtcta ttttgcccca gagtgtcaat gttagacttg acggacattg tgcagcgatc
     4861 tgctgctatc ctccggcaaa aaacaaaata taccttcgtt gcgccaatgt caccttttcc
     4921 gcagcgcaaa aactgtgtca gggaggacga ggtcgcacat tataacgatt tcgtagcaat
     4981 tggcagctaa atactggtct tatcagggaa gataatcaac agctaacatg taaataacct
     5041 tcaacaccgt gtaatttgca acaagccgca caattcacga aattagcgcc atcatcgacg
     5101 gaacttctta taacgtaagg tgaaaagctg taaagccagc cctgcgaaga tgaggggctg
     5161 caatgagata atcttcacag accacaaata atgtagggat gccaggatcg cgacaagata
     5221 gacgacatta cgcagcggtt gctggggatt acccaatttt cactgcatcg tctggataga
     5281 tgtgaatgct aaagcacgca aaatcaaccg actgacaatg ctcagcatta aataggttcg
     5341 agtggttaat ttgtagtcaa ataaaatcag attattcacc ccatgggacc agaaatcagt
     5401 aataggttaa gcgtagtgtc gcccaggcaa agcaccataa acctaacagg cgacggaggc
     5461 atatcaataa tggctgttta gaggagtatc aatgcgcatc aatttgtggt cagtcgaaat
     5521 catgactttc cagtttggat gatgttgtac aacttcctga ccaaggtgtt cgacgaaagc
     5581 tgtccggcta accgcttttc ccgttaaccg attgaataat gatgtcaaaa tgagttccct
     5641 ctcttattat tccttgctaa atggttagtt aaccttcacc agcgtgcgac cctggatctg
     5701 gttattaatg atggcctcgg caaagttcgg tgcctctgac agagatatct cttttgccgc
     5761 ctgggtatag aatgattccg gtaaatcggc gaccagtcgc tgccaggctt gtgcgcggcg
     5821 ttctggtggc gtcattactg aatccacccc ttgcaaacgg acattacgca gaataaatgg
     5881 catgaccgtg gttggcagag taaaaccacc cgccagacca caggccgcca cgcagccgcc
     5941 gtaattcatt tgcgccagca cttttgccag cactttgtcg ccaacagtgt caattgcccc
     6001 agcccagact tgtttttcca gaggacggga ttcggcaaac tcatcacgag ggagaatacg
     6061 actggcacct aaacttttca ggtattcatg ggtactttcg cgaccggaaa cggcaacgac
     6121 ctgataaccc aacttatgca gcagcgccac ggcggtactg ccgacgccac cactggcacc
     6181 cgtcacgaca atctccccgt cctgcgggcg aacaccggca tcttccagcg ccatcacaca
     6241 cagcatggcg gtaaaaccgg cagtaccgat aatcattgct ttacgcgcgt ccagcccttg
     6301 cggcatggca accagccagt cacctttcac tcgcgcctgc tccgccagcc caccccagtg
     6361 gttttcacca acgccccagc cagtgagtaa cacctcctga ccggcatgaa aacgcggatc
     6421 ttcgctggtg cgtacagttc cggcaaaatc gatcccagga atcatcggaa aattacggat
     6481 gatttttccc ttaccggtaa tcgccagcgc atctttatag ttcaggctcg accagtgaac
     6541 atcaaccgtg acatcgccct ccggcaggcg actttcgtca agtgtctgta ccgatgcgag
     6601 agttttgccg tcctgctgtt ctaaaagtaa cgcctgcata agtggtcctc atgtgcatga
     6661 tggattggaa aataatttct gaagactata ctcgctaatg gaaaagcaaa gccgatgaag
     6721 cgcaataaat tgcgagataa atctgatttg ctagtatgcc cgcttcctca ctatcggagt
     6781 taacacaagg atgagattaa cgacgaaatt ttcggccttt gttacgctgc tcaccgggtt
     6841 aacaattttt gtgactttgc tgggctgttc gctaagtttc tacaacgcaa ttcagtataa
     6901 gtttagtcat cgcgttcagg cggtggcgac ggcgattgat acccaccttg tgtcgaatga
     6961 cttcagcaca ttaaggccac aaattaccga attaatgatg tcggcagata tcgttcgtgt
     7021 agacctgctc catggtgata aacaggttta taccctggcc agaaatggta gttatcgtcc
     7081 ggttggctcc agcgatctgt ttcgcgaact gagtgttccg ttgataaagc atccggggat
     7141 gtcgctgcgt ctggtttatc aggatccgat gggcaactat ttccattctt taatgactac
     7201 cgcgccgctc acgggggcga ttggctttat cattgttatg ctcttcctgg cggtacgctg
     7261 gttacaacgg caacttgccg ggcaagaatt gctggaaacc cgggctactc gtatcttaaa
     7321 cggtgagcgt ggctctaatg tgttgggaac catctatgaa tggccgccca gaaccagcag
     7381 tgcgctagat acgctgcttc gtgaaattca gaacgcacgc gaacaacaca gccgtcttga
     7441 tacgctgatc cgctcttatg ccgcccagga cgtgaaaacc ggcctcaata accgactctt
     7501 tttcgataat cagttagcaa cgttactgga agatcaggag aaagtaggta cccacgggat
     7561 cgtgatgatg attcgtctgc cggatttcaa tatgttgagc gatacctggg ggcacagcca
     7621 ggttgaagaa cagttcttca ctctgacgaa tctgctgtcg acatttatga tgcgctaccc
     7681 tggcgcactg ctggcgcgtt accaccgcag tgattttgct gcgctgttac cgcaccggac
     7741 gttaaaagag gcagagagca tcgccggtca gttaatcaaa gccgtcgata ccttgccgaa
     7801 caataaaatg ctcgatcgcg acgatatgat ccacattggt atctgcgcct ggcgtagtgg
     7861 tcaggatacc gagcaggtaa tggaacatgc agagtctgcc acgcgtaatg cgggattgca
     7921 gggcggcaat agctgggcta tttacgatga ctcgttgcct gaaaaaggac gcggtaatgt
     7981 tcgctggcgt acgcttatcg agcaaatgct cagtcgcggc ggcccgcgcc tttatcaaaa
     8041 accggcggtt actcgcgaag gtcaggttca tcatcgcgaa ctcatgtgcc gcatcttcga
     8101 tggtaatgaa gaggttagct cggcggagta tatgccgatg gtcttgcagt ttggcttatc
     8161 ggaagagtat gaccgtctgc aaatcagccg tcttattcca ctattgcgtt actggccaga
     8221 ggaaaatctg gcgattcagg ttaccgttga gtcgctgatt cgcccgcgtt ttcagcgttg
     8281 gctgcgcgat acgttaatgc aatgtgaaaa atcacaacga aaacgcataa ttattgaact
     8341 tgcagaggcc gatgtaggtc aacatatcag tcgtttacaa cctgttattc gtttagtgaa
     8401 tgctttaggg gtacgggtag ccgtcaacca ggctggtttg acgctggtaa gcaccagttg
     8461 gatcaaagaa cttaatgttg agttactcaa gctccatccg gggctggtca gaaacattga
     8521 gaagcgaacg gagaaccagc tgctggttca aagcctggtg gaagcctgct ccgggaccag
     8581 cacccaagtt tacgccaccg gtgtgcgttc gcgaagcgag tggcagaccc tgattcagcg
     8641 cggtgttaca ggcgggcaag gggatttttt cgcgtcctca cagccacttg atactaacgt
     8701 gaaaaaatat tcacaaagat actcggttta acctgccgtt taatctgttt tcacgtagaa
     8761 taacgcgcgc tgcgtctcat gggagtgtgc ttgtctgctc gccagattgt tgcagcacat
     8821 atgcagatga atgaccttac gcggttgcaa acaggcgagg aatgctgctg atgcattaag
     8881 cctttctgga ctcaggcaga gatttgtaac aaaggaaacg aactgcacta attttcaccg
     8941 tagcagatga tttttgcgcc ttgtcgctgc tgcgtgtggt tggtaaagta agcggatttt
     9001 cttttccgcc ccagctttca ggattatccc ttagtatgtt gaaaaaattt cgtggcatgt
     9061 tttccaatga cttgtccatt gacctgggta ctgcgaatac cctcatttat gtaaaaggac
     9121 aaggcatcgt attgaatgag ccttccgtgg tggccattcg tcaggatcgt gccggttcac
     9181 cgaaaagcgt agctgcagta ggtcatgacg cgaagcagat gctgggccgt acgccgggca
     9241 atattgctgc cattcgccca atgaaagacg gcgttatcgc cgacttcttc gtgactgaaa
     9301 aaatgctcca gcacttcatc aaacaagtgc acagcaacag ctttatgcgt ccaagcccgc
     9361 gcgttctggt ttgtgtaccg gttggcgcga cccaggttga acgccgcgca attcgtgaat
     9421 ccgcgcaggg cgctggtgcc cgtgaagtct tcctgattga agaaccgatg gctgccgcaa
     9481 ttggtgctgg cctgccggtt tctgaagcga ccggttctat ggtggttgat atcggtggtg
     9541 gtaccactga agttgctgtt atctccttga acggcgtggt ttactcctct tccgtgcgca
     9601 ttggtggtga tcgtttcgac gaagctatca tcaactatgt gcgtcgtaat tacggttctc
     9661 tgatcggtga agccaccgca gaacgtatca agcacgaaat cggttcggct tacccgggcg
     9721 atgaagtccg tgaaatcgaa gttcgtggcc gtaacctggc agaaggtgtt ccacgcggtt
     9781 ttaccctgaa ctccaacgaa atcctcgaag cactgcagga accgctgacc ggtattgtga
     9841 gcgcggtaat ggttgcactg gaacagtgcc cgccggaact ggcttccgac atctccgagc
     9901 gcggcatggt gctcaccggt ggtggcgcac tgctgcgtaa ccttgaccgt ttgttaatgg
     9961 aagaaaccgg cattccagtc gttgttgctg aagacccgct gacctgtgtg gcgcgcggtg
    10021 gcggcaaagc gctggaaatg atcgacatgc acggcggcga cctgttcagc gaagagtaat
    10081 cggatgcagg caggggaaat gtctgtttac cctgcctggt ctgatacgag aatacgcata
    10141 acttatgaag ccaattttta gccgtggccc gtcgctacag attcgcctta ttctggcggt
    10201 gctggtggcg ctcggcatta ttattgccga cagccgcctg gggacgttca gtcaaatccg
    10261 tacttatatg gataccgccg tcagtccttt ctactttgtt tccaatgctc ctcgtgaatt
    10321 gctggatggc gtatcgcaga cgctggcctc gcgtgaccaa ctagaacttg aaaaccgggc
    10381 gttacgtcag gaactgttgc tgaaaaacag tgaactgctg atgcttggac aatacaaaca
    10441 ggagaacgcg cgtctgcgcg agctgctggg ttccccgctg cgtcaggatg agcagaaaat
    10501 ggtgactcag gttatctcca cggttaacga tccttatagc gatcaagttg ttatcgataa
    10561 aggtagcgtt aatggcgttt atgaaggcca gccggtcatc agcgacaaag gtgttgttgg
    10621 tcaggtggtg gccgtcgcta aactgaccag tcgcgtgctg ctgatttgtg atgcgaccca
    10681 cgcgctgcca atccaggtgc tgcgcaacga tatccgcgta attgcagccg gtaacggttg
    10741 tacggatgat ttgcagcttg agcatctacc agtgaatacg gatattcgtg ttggtgatgt
    10801 gctggtgact tccggtctgg gcggtcgttt cccggaaggc tatccggtcg cggttgtctc
    10861 ttccgtaaaa ctcgataccc agcgcgctta tactgtgatt caggcgcgtc cgacagcagg
    10921 gctgcaacgt ttgcgttatc tgctgctgct gtggggcgcg gatcgtaacg gcgctaaccc
    10981 gatgacgccg gaagaggtgc atcgtgttgc taatgaacgt ctgatgcaga tgatgccgca
    11041 ggtattgcct tcgccagacg cgatggggcc aaagttacct gaaccggcaa cggggatcgc
    11101 tcagccgact ccgcagcaac cgacgacagg aaatgcagct actgcgcctg ctgcgccgac
    11161 acagcctgct gctaaccgct ctccacaaag ggctacgccg ccgcaaagtg gtgctcaacc
    11221 gcctgcgcgt gcgccgggag ggcaatagtg gcgagctatc gtagccaggg acgttgggta
    11281 atctggctct ctttcctcat tgcgctgttg ctgcaaatca tgccctggcc ggataacctg
    11341 attgttttcc ggccaaactg ggtgttactc atcttgttgt attggatcct ggccttgcct
    11401 catcgcgtaa atgtgggcac aggttttgtg atgggtgcca tactggatct gatcagcggc
    11461 tcgacgcttg gcgtacgcgt attggcgatg agcatcattg cttacctggt ggcgctgaaa
    11521 taccagcttt tccgcaacct cgcattatgg cagcaggcgc tggtcgtcat gttgctttcg
    11581 ctggtggtgg atattattgt tttctgggca gagtttttag tgattaacgt ctctttcaga
    11641 ccggaagtgt tctggagtag tgtagtcaat ggggtgctct ggccgtggat tttcttgctg
    11701 atgcgtaaag tccgtcagca gtttgcagtg caataaaggt ttctatgact tctctgtatt
    11761 tagcttccgg ttctccgcgt cgtcaggagt tacttgcgca acttggcgtg acctttgaac
    11821 gtattgttac gggcattgag gagcagcgtc agccgcagga gagcgcgcag cagtatgttg
    11881 tgcgtctggc gcgcgagaaa gcacaggcgg gtgtcgcgca aacggcgcag gatctcccgg
    11941 tgctgggtgc ggatactatc gttatcctga atggagaagt gctggagaaa ccgcgcgacg
    12001 cagagcatgc ggcgcagatg ttgcgcaaat tatcgggtca gacccatcag gtgatgacgg
    12061 cagtggcgtt ggccgacagc cagcacattc tcgattgcct ggtggtcacc gatgtgactt
    12121 tcagaacgtt aacagacgaa gacatcgcgg gctatgtcgc cagcggtgaa ccgttagata
    12181 aagcaggtgc atacggtatt caggggctgg gtggctgttt tgtcaggaag ataaatggca
    12241 gctatcacgc cgtagtcggc ttaccgctgg ttgaaacgta tgaattatta agtaatttta
    12301 acgcactgcg tgagaaaagg gataaacatg acggctgaat tgttagtaaa cgtaacgcct
    12361 tcggaaacgc gagtggcgta tattgatggc ggtattctgc aggaaattca tattgaacgt
    12421 gaggcgcgac gcggaatagt aggcaatatc tacaagggtc gtgtaagtcg tgtacttccg
    12481 ggtatgcagg cggcttttgt agatattggg ctggataaag ccgcgtttct tcatgcatcc
    12541 gatatcatgc cgcacaccga atgtgtggcg ggtgaagaac aaaagcaatt cacggtgcgc
    12601 gacatctcgg aactggttcg tcaggggcaa gatctgatgg tgcaggtggt gaaagatccg
    12661 cttggcacta agggggcgcg cctgaccacc gatatcacgc tgccttctcg ctatctggtc
    12721 tttatgccag gggcttctca cgttggtgtt tcccaacgta ttgaaagcga atcagaacgt
    12781 gaacgcctga aaaaagtggt cgcagagtat tgcgacgagc agggcgggtt tatcatccgt
    12841 accgcagcgg aaggggttgg cgaggctgaa ctggcctccg atgccgctta tctgaaacgc
    12901 gtctggacca aagttatgga gcgtaaaaaa cgcccgcaga cccgttatca gctgtacggc
    12961 gaactggcgc tggcgcagcg tgttctgcgt gatttcgccg acgccgaact ggaccgcatt
    13021 cgcgttgact cacgcctgac ttacgaagca ttgctggagt ttacctcgga gtacattccc
    13081 gagatgacca gcaagctgga gcattacacc ggacgccagc cgattttcga tctctttgat
    13141 gtcgaaaacg aaatccagcg agcgctggaa cgcaaagtag aactgaaatc cggtggttat
    13201 ctgattatcg accagaccga agcgatgacc accgtggaca tcaataccgg agcgtttgtc
    13261 ggtcatcgca atctggacga caccattttc aataccaata ttgaagcgac gcaggctatc
    13321 gctcgccagt tacggttgcg taatctgggt gggattatca ttattgattt catcgatatg
    13381 aataatgaag atcaccgccg ccgcgtgctg cactcgctgg agcaggcgtt gagcaaagac
    13441 cgggtgaaaa ccagcgttaa tggtttttcg gcgctggggc tggtggagat gacgcgtaaa
    13501 cgcacccgcg aaagcattga gcacgtactg tgtaacgaat gcccaacctg ccacggtcgc
    13561 ggaacggtga aaaccgtgga aacggtatgc tatgaaatca tgcgcgagat tgttcgtgtc
    13621 caccatgctt acgactccga ccgtttcctg gtctatgctt ctccggcagt agctgaagcc
    13681 ttgaaaggcg aagagtcaca ctcgctggcg gaagtggaaa ttttcgttgg taaacaggtt
    13741 aaagtacaaa ttgaaccgct ctataaccag gagcagtttg acgtcgtaat gatgtaaaca
    13801 gatgctgggc cgccatccgg caaagggttt ttgagtcaca tttttagcag acaaggagtg
    13861 acgggtgagg cgattgccgg ggattttact gcttactgga gccgcgctcg ttgtgatcgc
    13921 agccctgctg gttagcggcc tgcgtattgc tttaccgcat cttgacgcct ggcgtccgga
    13981 aatcctcaac aaaatagaat ccgcgactgg catgccggta gaagccagtc agctctcagc
    14041 cagctggcag aattttggcc cgacgcttga agcacacgac atccgtgcag aactaaaaga
    14101 tggcggcgaa ttttcggtta aacgcgttac tctggcgctg gatgtctggc agagcctgtt
    14161 acatatgcgc tggcagtttc gcgacctcac tttctggcag ctgcgctttc gcaccaacac
    14221 tcctatcacc agcggtggta gcgatgacag tctggaagcc agtcacatca gcgatctgtt
    14281 tcttcgtcaa tttgaccatt tcgatctccg cgacagtgaa gtcagttttc tgacgccatc
    14341 cggtcagcgc gccgagctgg cgatcccaca actcacctgg ctgaacgatc cacgtcgaca
    14401 ccgtgcggaa ggcctggtaa gcctctccag ccttaccgga cagcacggcg tgatgcaggt
    14461 gcgcatggat ttgcgcgatg atgaggggtt gttaagcaat ggtcgcgtct ggctacaggc
    14521 ggatgacatc gacctgaagc cgtggctcgg taaatggatg caggacaata ttgctctgga
    14581 aacggcacag ttctcccttg aaggctggat gacgatcgac aaaggcgatg taaccggcgg
    14641 tgacgtctgg ctgaaacagg gcggtgccag ctggttgggc gagaaggaaa cgcatacgct
    14701 gtcggtggat aatctgaccg cgcatattac gcgtgaaaat ccgggctggc agttctctat
    14761 tcccgataca cggatcacga tggacggtaa accctggccg agcggagcat tgacgctggc
    14821 ctggataccg gaacaggacg ttggcggcaa agacaataaa cgcagtgacg aactccggat
    14881 tcgcgccagt aatctggagc tggcaggcct ggagggcata cgcccgctgg ccgcgaaact
    14941 ttcacctgca ctgggtgatg tttggcgctc cacacaaccg agcggcaaga ttaacactct
    15001 ggcgctggat atcccgcttc aggcggcaga caatacccgt tttcaggcat cgtggagcga
    15061 tctggcctgg aagcaatgga aattattacc gggtgcggaa cacttctccg ggacgctttc
    15121 cggcagcgtt gaaaatggtt tgcttaccgc gtcgatgaag caggcaaaga tgccttacga
    15181 aacggtattc cgtgcgccac tggaaatcgc cgacggccag gcaactataa gctggctgaa
    15241 caatgacaaa ggtttccagc tggatgggcg taatattgac gttaaagcca aagccgtcca
    15301 tgcgcgcggc ggttttcgtt acctgcaacc tgctaacgat gaaccctggc tgggtattct
    15361 ggctggcatc agtaccgatg atggttcaca agcctggcgc tatttcccgg aaaacttgat
    15421 gggtaaagac ctggttgatt acttaagtgg cgcgattcag ggcggtgaag cggataacgc
    15481 gacgctggtt tatggtggca atccgcaact cttcccctat aaacacaacg aaggtcagtt
    15541 tgaagtgctg gtgccgctgc gcaacgcgaa gtttgccttc cagccggact ggcctgcatt
    15601 aactaacctt gatattgaac tggactttat taacgacggt ttatggatga aaaccgatgg
    15661 cgttaatctg ggcggcgtgc gcgcgagtaa tctcaccgca gtgatccctg actactcaaa
    15721 agaaaaactg ctgattgacg ctgacattaa aggtccgggt aaagccgtag gcccttactt
    15781 tgatgagaca ccgctgaaag attccctggg tgcgaccctg caagaactcc agctcgacgg
    15841 cgatgtgaat gctcgcttac atcttgatat cccgctgaac ggcgagttgg taaccgcgaa
    15901 aggtgaagtg acgctgcgta ataacagtct gtttatcaaa ccactcgaca gcaccctgaa
    15961 aaatttgagc ggtaaattca gctttatcaa tggcgatctg caaagtgaac cactgacagc
    16021 aagctggttt aatcagccgt tgaacgtgga tttttccacc aaagaagggg caaaagccta
    16081 ccaggtagcg gtaaacctca acggtaactg gcaaccggcg aaaaccggcg ttctgcctga
    16141 agcggtgaac gaagcattga gtggcagcgt ggcgtgggat ggtaaagtgg gcattgatct
    16201 gccttatcat gctggcgcga cctataacgt agagatgaac ggcgatctga ataatgtgag
    16261 tagtcactta ccttcaccgt tagccaaacc tgcgggtgaa ccactagcgg taaacgttaa
    16321 ggttgatggc aatctcaaca gctttgaatt aaccggacag gctggtgcgg ataaccattt
    16381 caatagccgc tggttgcttg gtcaaaagct gacgctcgat cgtgctattt gggcggcaga
    16441 cagtaaaacg ctcccgccgt tgccggaaca aagtggcgtt gaactcaata tgccgccgat
    16501 gaatggtgcc gagtggctgg ccctgttcca gaaaggcgct gcggagagtg tcggtggtgc
    16561 agcgagtttc ccacaacaca taacgttacg tacgcctatg ttgtcactgg gaaatcagca
    16621 atggaataac ctgagtattg tttcgcaacc gacggcaaat ggcaccctgg ttgaggcgca
    16681 agggcgtgaa atcaacgcca cgctagcgat gcgtaataac gcgccgtggc tggcgaatat
    16741 caaatatctt tattacaacc cgagcgtggc gaaaactcgt ggtgattcaa cgccgtcatc
    16801 acctttcccg acaacggagc gcattaactt ccgtggctgg ccggacgcac aaatacgatg
    16861 cacagagtgc tggttctggg ggcaaaaatt cggtcgcatt gacagtgata tcaccatttc
    16921 tggcgatacg ttaacgctga ccaatggact gattgatact ggtttctcgc ggcttactgc
    16981 cgatggtgaa tgggttaata atccggagaa tgaacgtacc tcgctgaaag gaaaactgcg
    17041 cgggcagaaa attgatgccg ccgcagaatt ttttggtgtc acgacgccca tacgccagtc
    17101 gtcatttaat gtggattacg atttacactg gcgcaaagca ccctggcaac cagatgaggc
    17161 gacgttgaat ggcatcattc atactcaact gggtaaaggc gaaattaccg aaatcaatac
    17221 cggacatgcc gggcaattgc tgcgcttatt gagcgttgat gccctgatgc gtaagctgcg
    17281 ttttgatttc agagacactt ttggcgaagg gttctatttt gactccattc gcagcaccgc
    17341 gtggattaaa gacggggtta tgcacaccga cgacacgctg gtggatggcc tggaggcgga
    17401 tatcgccatg aaagggtcgg taaatctggt acgtcgcgac ctgaatatgg aagcggttgt
    17461 cgcaccagag atttctgcga cggtgggcgt ggctgcggct tttgcggtta accccattgt
    17521 tggcgcggca gtatttgcag ccagtaaagt gctggggccg ctgtggagca aagtctccat
    17581 tttgcgctat cacatttcgg gtccgctgga cgatccgcaa atcaacgaag tgttgcgcca
    17641 accgcgtaaa gaaaaagcgc aatgatttga cgagggcgcg taattgcccc aatctcatat
    17701 gataatcgtt gccaaaggcc aacgagccgg aacataactg taggccggat agggcgttca
    17761 cgccgcatcc ggcagccgta aaaaatcctc tactgcagta actaacgagt agcaaaaacg
    17821 atgagtctga acctggtaag tgaacaattg ctagcggcga acggcctgaa acatcaggac
    17881 ttgttcgcga tcctcggtca actggccgaa cgtcgccttg attatggcga tctctatttt
    17941 cagtcgagct atcacgaatc ctgggtttta gaagaccgca ttattaaaga tggttcttac
    18001 aacatcgatc agggcgttgg tgtgcgtgca atcagtggtg aaaaaaccgg atttgcttac
    18061 gctgaccaaa tcagcctgct ggcgctggaa cagagtgcgc aagcggcgcg caccatcgtc
    18121 cgtgatagtg gtgatggcaa agtacagacg ctgggcgcgg tagagcatag cccgttgtat
    18181 acctcggtag atccgctgca aagcatgagc cgtgaagaga agctggatat cctgcgtcgc
    18241 gtcgataagg ttgcccgcga agcggacaag cgcgtacaag aagtgactgc cagcctcagc
    18301 ggcgtttatg aattaattct ggttgcggcc accgacggca cgctggcggc ggatgttcgt
    18361 ccgctggtgc gtctttccgt gagcgttctg gtcgaagaag atggcaaacg cgaacgcggt
    18421 gccagtggcg gcggcggtcg ttttggttat gagttcttcc ttgccgatct tgacggcgaa
    18481 gtccgtgcgg atgcatgggc aaaagaagca gtgcgtatgg cgctggtcaa tctttctgcc
    18541 gttgctgcac cagcgggcac catgccggta gtacttggcg caggttggcc gggcgtgctg
    18601 ttgcatgaag cggtaggtca cggtctggaa ggcgacttca accgccgcgg cacttcagta
    18661 tttagtgggc aggtcgggga gctggtggct tcagaactgt gtaccgtggt tgatgacggc
    18721 acgatggtcg atcgccgtgg ttcggtggcg attgatgacg aaggtacgcc aggccagtac
    18781 aacgtgctga ttgagaacgg cattctgaaa ggctacatgc aggataaact caacgcgcgt
    18841 ttgatgggga tgacgccgac tggcaacggt cgccgtgaat cctacgccca tctgcccatg
    18901 ccgcgtatga ccaacaccta tatgctgccg ggtaaatcga ccccgcagga aattattgaa
    18961 tccgttgagt acggtatcta tgcaccgaac tttggtggcg gtcaggtgga tattacatcc
    19021 ggcaaattcg ttttctccac ttcagaagcg tatctgattg aaaacggtaa agtaacgaag
    19081 ccggtgaaag gcgcgacgtt gattggttcc ggtatcgaaa ccatgcagca gatttcgatg
    19141 gttggcaacg acctgaaact ggataacggc gtgggtgtct gcggtaaaga agggcaaagt
    19201 ttgccggttg gcgtgggcca gccaacgctg aaagttgata acctgactgt tggcggtact
    19261 gcgtaataat ttcattattt tcagaaggat aattaatctt tctacgtgca cgaacggtcc
    19321 cctcgctcct ctggggttag ggtgagggga acccgttggc acaggtttgt agaacgtaac
    19381 agtacaatat gaattacttc tctttcccgc gcccgtgcat ctcctgaaac aatttaccga
    19441 cctcaacaaa ataatccgtc agcgagttga tcacgacctg taccttcagc ggcagcttat
    19501 ctttttcggt atataacgca taaaccgggc gtggatctga ctgataacgc ggcagcagga
    19561 tctccagctc accgcgattg atctcgttga tcacccacat cagcggcacg taggcgatcc
    19621 cggcacccgc cgttagccag cgcaccagcg tcatcggatc attagtcaca aatctccctt
    19681 gcgggatcag gcgagtcgag atcccttccg gtgcaatcag ttcaaattca ttgtcgggcc
    19741 gcacgctgta ttcaagccat gaatggctac ttaaatcggc gggtttttcc ggtatgccgt
    19801 attgtgtgag atagcttttc gcggcgcaca ccaccattgg catcgcgcca agacggcggg
    19861 aaaacaggct ggaatcctgc aacgcgccga cgcggatcac cacatccaga ccgtcggcaa
    19921 tcaggtcggg ggctggaatt ccggtaacca gattgacgct caaacctggg tattctttca
    19981 gcattttggc tgtcagcccg gcgagaacat tttgtgccat agttgaagaa cagccaatgc
    20041 gtagcgtccc gatgggggtg ttattgaagg catacagttg ctcatgaaca tcctgcactt
    20101 catgaagcat acgacggcag ccctggtagt aaattctacc ggcttcggtc aggccaatgc
    20161 tgcgtgtgct acggtttaac agctttacct gcaactcatc ttccagtttt gacaccgtct
    20221 gactgatgga cgaaacgctc atctgtagct gtctggcggc ggcggtaaaa gagccaaatt
    20281 caactacttt ggcaaacacc gacatgcgtt ttagtcgttc cattattcac tctgacttaa
    20341 aagtgattta gatcacataa tatagataac agcataacag ttacgctaat atattaaata
    20401 tcaatctaca gcaatgttgc tctcgcccgg ctttccatgc ccttctctgc ggcgacagat
    20461 gctgaaaata ataacgcctg ctctctctat acaaccaagg tcaacatgag tctgtttccc
    20521 gttatcgtgg tgtttgggct gtccttccca ccgatatttt ttgaattgct tttatcactg
    20581 gcgattttct ggctggtgcg ccgggtactt gtgccaacag gtatctacga ctttgtctgg
    20641 catccggcgt tgttcaacac cgcgctctat tgctgcttgt tttatttgat atcgcgactg
    20701 ttcgtttgag gttgaagtga aaacactaat aagaaaattc tcccgtacgg ccatcacggt
    20761 cgtattagtc attctggcct tcatcgcaat ttttaatgcc tgggtctatt acaccgaatc
    20821 cccctggacg cgtgacgcgc gctttagcgc tgacgtcgtt gcgatcgcgc cggacgtttc
    20881 tggactcatt acccaggtga atgttcatga taaccagctg gtgaaaaaag gacagatact
    20941 gttcaccatc gaccagccgc gctatcaaaa ggcgcttgag gaagcgcaag ccgatgttgc
    21001 ttattatcag gtactggcac aggagaaacg ccaggaggcc ggacgtcgta accgtctcgg
    21061 tgtgcaggcg atgtctcgcg aagagatcga ccaggctaac aacgtactac aaacggttct
    21121 gcatcagtta gcgaaagcgc aggcgacccg cgatctggca aaactggatc ttgaacgcac
    21181 ggtgatccgc gcgccagcag atggctgggt gaccaacctc aacgtctata ccggtgagtt
    21241 tattactcga ggatcaacgg cggttgcgct ggtgaaacag aactccttct atgtactggc
    21301 ctatatggaa gaaactaagc tggaaggggt gcgtccgggg tatcgtgcag agatcacgcc
    21361 gcttggcagt aacaaagtgc tgaaagggac tgttgatagt gttgccgctg gggtcaccaa
    21421 cgccagcagc acgcgtgacg acaaagggat ggcgaccatc gactctaacc ttgaatgggt
    21481 gcgtttggcg caacgtgttc cggttcgtat tcgtctcgac aaccagcaag agaacatctg
    21541 gcctgcgggc accactgcta cagtggtggt cactggcaaa caagatcgcg acgaaagcca
    21601 ggattcgttc ttccgtaaaa tggcccatcg cctgcgtgag tttggttaat cacgatgggt
    21661 attttctcca ttgctaacca acatattcgc tttgcggtaa aactggcgac cgccattgta
    21721 ctggcgctgt ttgttggctt tcacttccag ctggaaacgc cacgctgggc ggtactgaca
    21781 gcggcgattg ttgccgccgg tccggccttt gctgcgggag gtgaaccgta ttctggcgct
    21841 attcgctatc gtggcttttt gcgcatcatc ggcacattta ttggctgtat tgccggactg
    21901 gtgatcatca ttgcgatgat ccgcgcacca ttattgatga ttctggtgtg ctgtatctgg
    21961 gccggttttt gtacctggat atcctcgctg gtacgaatag aaaactcgta cgcgtggggg
    22021 ctggccggtt ataccgcgct gatcattgtg atcaccattc agccggaacc attgcttacg
    22081 ccgcagtttg ccgtcgaacg ttgtagcgag atcgttatcg gtattgtgtg tgcgattatg
    22141 gcggatttgc tcttttctcc gcgatcgatc aaacaagaag tggatcgaga gctggaaagt
    22201 ttgctggtcg cgcaatatca attaatgcaa ctctgtatca agcatggcga tggtgaagtt
    22261 gtcgataaag cctggggcga cctggtgcga cgcaccacgg cgctacaagg catgcgcagc
    22321 aacctgaata tggaatcttc ccgctgggcg cgggccaatc gacgtttaaa agcgatcaat
    22381 acgctatcgc tgacgctgat tacccaatcc tgcgaaactt atcttattca gaatacgcgc
    22441 ccggaattga tcactgatac tttccgcgaa ttttttgaca cgccggtaga aaccgcgcag
    22501 gacgtccaca agcagctcaa acgcctgcgg agagttatcg cctggaccgg ggaacgggaa
    22561 acgcctgtca ccatttatag ctgggtcgcg gcggcaacgc gttatcagct tctcaagcgc
    22621 ggcgttatca gtaacacaaa aatcaacgcc accgaagaag agatcctgca aggcgaaccg
    22681 gaagtcaaag tagagtcagc cgaacgtcat catgcgatgg ttaacttctg gcgaaccaca
    22741 ctttcctgca ttctgggaac gcttttctgg ctgtggacgg gctggacttc cggcagtggt
    22801 gcaatggtga tgattgcggt cgtgacgtca ctggcaatgc gtttgccgaa tccacgcatg
    22861 gtggcgatcg actttatcta cgggacgctg gccgcgctgc cgttagggct gctctacttt
    22921 ttggtgatta tccctaatac ccaacagagc atgttgctgc tgtgtattag cctggcagtg
    22981 ctgggattct tcctcggaat agaagtacag aaacggcgac tgggctcgat gggggcgctg
    23041 gccagcacca taaatattat cgtgctggat aacccgatga ctttccattt cagtcagttt
    23101 ctcgacagcg cattagggca aatcgtcggc tgtgtgctcg cgttcaccgt tattttgctg
    23161 gtgcgggata aatcgcgcga caggactgga cgtgtactgc ttaatcagtt tgtttctgcc
    23221 gctgtttccg cgatgactac caatgtggca cgtcgtaaag agaaccacct cccggcactt
    23281 tatcagcagc tgtttttgct gatgaataag ttcccagggg atttgccgaa atttcgcctg
    23341 gcgctgacga tgattatcgc gcaccagcgc ctgcgtgatg cgccgatccc ggttaacgag
    23401 gatttatcgg cgtttcaccg acaaatgcgc cgcacagcag accatgtgat atctgcccgt
    23461 agcgatgata aacgtcgtcg gtactttggc cagttgctgg aagaactgga aatctaccag
    23521 gaaaagctac gcatctggca agcgccaccg caggtgacgg aaccggttca tcggctggcg
    23581 gggatgctcc ataagtatca acatgcgttg accgatagtt aagtcaaaac cgacgccaaa
    23641 agcgtcggtt ttttcatggc tatacttagc gatgcacggc agaactcgcc gcgaaacgtg
    23701 acggtggcaa cagatgaata tttatacctt tgattttgat gagattgaga gtcaggagga
    23761 tttttatcgt gactttagcc aaacctttgg tctggcgaaa gataaggtac gcgatctcga
    23821 ctcactatgg gatgtgttaa tgaacgatgt cctgccgcta ccacttgaga ttgaatttgt
    23881 tcatctggga gagaaaacgc gtcgccgttt tggcgcgtta atattgctgt ttgatgaggc
    23941 agaggaagag ctggaagggc atttgcgttt taatgttcgt cattagcgca aaaaaagccc
    24001 ccgaaccggg ggcaatatcg tcggacaaga cgatgagggt ttatttgtac agttcagccg
    24061 tagcgtgcca ggtgtcaccg ctacgagctt cagtaatctg gtaggccgtt gcgcctttct
    24121 cttccgcttt tttgttcagc atttcacgca tatccattgg cgaagacgcc acaccactta
    24181 cggatacggt cccgattgct tcacgatttt gtgcttgtgc agcatcaatg gagtcggcag
    24241 cgaatgcacc gaaagagaga acagaaagta cgcttaatgc agctacagtg gttttgattt
    24301 tcatgatttt tacctcgaca taatctttta gctgggcctt tgtttagtga cccatatcac
    24361 aaaatcaagt atacactaat tattgaacta attaatacca cgct
//
LOCUS       DAZFXT010000068        24383 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.068, whole genome shotgun sequence.
ACCESSION   DAZFXT010000068 DAZFXT010000000
VERSION     DAZFXT010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24383
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.068"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            116..415
                     /locus_tag="ACHKW0_002917"
     CDS             116..415
                     /locus_tag="ACHKW0_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475718.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILALFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVFPVTNIVLFLILIFFKKR"
     gene            complement(462..1349)
                     /gene="rarD"
                     /locus_tag="ACHKW0_002918"
     CDS             complement(462..1349)
                     /gene="rarD"
                     /locus_tag="ACHKW0_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HFO7475719.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1401..1868)
                     /gene="yigI"
                     /locus_tag="ACHKW0_002919"
     CDS             complement(1401..1868)
                     /gene="yigI"
                     /locus_tag="ACHKW0_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase YigI"
                     /protein_id="HFO7475720.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            2033..2902
                     /gene="pldA"
                     /locus_tag="ACHKW0_002920"
     CDS             2033..2902
                     /gene="pldA"
                     /locus_tag="ACHKW0_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004620 - phospholipase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HFO7475721.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            3035..4864
                     /gene="recQ"
                     /locus_tag="ACHKW0_002921"
     CDS             3035..4864
                     /gene="recQ"
                     /locus_tag="ACHKW0_002921"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HFO7475722.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFSEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4928..5548
                     /gene="rhtC"
                     /locus_tag="ACHKW0_002922"
     CDS             4928..5548
                     /gene="rhtC"
                     /locus_tag="ACHKW0_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HFO7475723.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5610..6230)
                     /gene="rhtB"
                     /locus_tag="ACHKW0_002923"
     CDS             complement(5610..6230)
                     /gene="rhtB"
                     /locus_tag="ACHKW0_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HFO7475724.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6341..7363
                     /gene="pldB"
                     /locus_tag="ACHKW0_002924"
     CDS             6341..7363
                     /gene="pldB"
                     /locus_tag="ACHKW0_002924"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HFO7475725.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7371..8171
                     /gene="yigL"
                     /locus_tag="ACHKW0_002925"
     CDS             7371..8171
                     /gene="yigL"
                     /locus_tag="ACHKW0_002925"
                     /EC_number="3.1.3.23"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HFO7475726.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8247..9146
                     /gene="bioP"
                     /locus_tag="ACHKW0_002926"
     CDS             8247..9146
                     /gene="bioP"
                     /locus_tag="ACHKW0_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HFO7475727.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(9034..9987)
                     /gene="metR"
                     /locus_tag="ACHKW0_002927"
     CDS             complement(9034..9987)
                     /gene="metR"
                     /locus_tag="ACHKW0_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HFO7475728.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10110..12371
                     /gene="metE"
                     /locus_tag="ACHKW0_002928"
     CDS             10110..12371
                     /gene="metE"
                     /locus_tag="ACHKW0_002928"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HFO7475729.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12410..13225)
                     /gene="ysgA"
                     /locus_tag="ACHKW0_002929"
     CDS             complement(12410..13225)
                     /gene="ysgA"
                     /locus_tag="ACHKW0_002929"
                     /EC_number="3.-.-.-"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HFO7475730.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13487..14248
                     /gene="udp"
                     /locus_tag="ACHKW0_002930"
     CDS             13487..14248
                     /gene="udp"
                     /locus_tag="ACHKW0_002930"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016763 - pentosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HFO7475731.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14389..15816
                     /gene="rmuC"
                     /locus_tag="ACHKW0_002931"
     CDS             14389..15816
                     /gene="rmuC"
                     /locus_tag="ACHKW0_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HFO7475732.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15911..16666
                     /gene="ubiE"
                     /locus_tag="ACHKW0_002932"
     CDS             15911..16666
                     /gene="ubiE"
                     /locus_tag="ACHKW0_002932"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HFO7475733.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16680..17285
                     /gene="ubiJ"
                     /locus_tag="ACHKW0_002933"
     CDS             16680..17285
                     /gene="ubiJ"
                     /locus_tag="ACHKW0_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HFO7475734.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17282..18922
                     /gene="ubiB"
                     /locus_tag="ACHKW0_002934"
     CDS             17282..18922
                     /gene="ubiB"
                     /locus_tag="ACHKW0_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HFO7475735.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            19001..19270
                     /gene="tatA"
                     /locus_tag="ACHKW0_002935"
     CDS             19001..19270
                     /gene="tatA"
                     /locus_tag="ACHKW0_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HFO7475736.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19274..19789
                     /gene="tatB"
                     /locus_tag="ACHKW0_002936"
     CDS             19274..19789
                     /gene="tatB"
                     /locus_tag="ACHKW0_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HFO7475737.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19792..20568
                     /gene="tatC"
                     /locus_tag="ACHKW0_002937"
     CDS             19792..20568
                     /gene="tatC"
                     /locus_tag="ACHKW0_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HFO7475738.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20610..21392
                     /gene="tatD"
                     /locus_tag="ACHKW0_002938"
     CDS             20610..21392
                     /gene="tatD"
                     /locus_tag="ACHKW0_002938"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HFO7475739.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21389..21877)
                     /gene="rfaH"
                     /locus_tag="ACHKW0_002939"
     CDS             complement(21389..21877)
                     /gene="rfaH"
                     /locus_tag="ACHKW0_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HFO7475740.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            22044..23537
                     /gene="ubiD"
                     /locus_tag="ACHKW0_002940"
     CDS             22044..23537
                     /gene="ubiD"
                     /locus_tag="ACHKW0_002940"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HFO7475741.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23583..24284
                     /gene="fre"
                     /locus_tag="ACHKW0_002941"
     CDS             23583..24284
                     /gene="fre"
                     /locus_tag="ACHKW0_002941"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HFO7475742.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
BASE COUNT         5750 a         6051 c         6669 g         5913 t
ORIGIN      
        1 ctatacagta gtaatttaga gtgtgtaagt ttttattgat ggggtatatt ttaattaaat
       61 ataattgaaa tgtattttta ttctataaac aatatcttgg gtgaatggat tctatatggg
      121 aaatggtaac ttttggcagt tgttcttggg taaagaacct tacagcaaaa aatctgattt
      181 tgttaaggca tggttattgt ggtgggctat taccatcctt gctctattta ttttaagcct
      241 gactacgaca ttaaatttaa gaagtatagg tatagggtta agttttgggt tttgtcaaat
      301 ccttggtaga tatacgatgt ctctaggatg gagtaagtac tggggagttt tgggggtatt
      361 tcctgtaaca aatattgttc tttttcttat cttaattttt ttcaaaaaaa gataaataag
      421 tcagtgggta ggctctttaa acggcctacc catgttaatt cttaactttt tctacgctgc
      481 gtataaatcg catccatcac aaaaattgcc agcgccaccc aaataaaggc gaaagtcacc
      541 atcttatcgg cacccggttt ttcaccataa aacgtcacag ccagcaggaa catcagcgtc
      601 gggccaatgt actggaaaaa gcctaacgtt gagagacgca agcgcgtggc agcggcggta
      661 aaacacaaca gcggtacggt agtgacaata ccggcggcga tcagcagtaa attcagcgac
      721 atcgggtttt gccccatatg gctggttgag ctgtcggcaa tagcaaacag gtaaattgcc
      781 gccacgggca gcagccacat ggtttcgatt aacatgccgg tttgcgcttc aacggcaatc
      841 ttcttgcgta ccagaccgta gaaggcaaaa ctaaatgcca gtcccagcgc gataataggt
      901 agcgaaccaa aagtccacag ctggactaac acgccacata tcgccagaat caccgccagc
      961 cattgcatcc ggcggaatcg ctcgccgagg aaaatcatcc ccagcacaat gttcaccagc
     1021 gggttaataa agtaaccaag gctcgcttcc agcatatggt gattgttcac cgcccagata
     1081 aacagtagcc agttgccacc aatcagcacg gcagagactg ccagcataaa aattttctgt
     1141 ggcgtctgaa tcagcgtttt taaataggac cactggcggc aaatgctcat cagcaccacc
     1201 ataaagaaaa acgaccagat cacgcgatgc gtcaggattt catcggcagg cacgtagtaa
     1261 atcaacttga agtacgctgg cgctataccc caaataaaat aagcggcaag agcgagtaat
     1321 acgccctgcc gcgtttgttt tgcatccatc gggaatactc atttttaatt ggtaacagca
     1381 gtttacctgc ttttatgtct tcaacctacc atataggtgg cggtggcact ggcaatataa
     1441 agctgttctt cattgtgtaa ttcaacgcgg gcgacggcga ctttattgcc tgcacgcaac
     1501 aggctactgg tagcagtaaa acgctcgccc ctgcctgggc gcagataatc aacgcgaaga
     1561 tcaatggtcc ccatccgcga tagccgctgg cgtagttcat cttcactgat ggtttcgtgg
     1621 cgggttaagg tacttcccac gcacaccaga ccggcggcga catccagcgc cgacgcaatg
     1681 accccgccgt gcaaaatgct ttgcgcccag ttgcccacca tcattggctg atttttaaag
     1741 gccagctgtg cgaactcttt ttcgtaacgc tccagttcca tccccaatgc gcggttaaat
     1801 ggcatgtgat aaacaaacat ctcacccact aatttcaggg cttgttcagc ggtcagtacg
     1861 gcagacatat catccttaca cttcattggt taatgaaatg ttgattttat gcttctttgt
     1921 tgttggtttc tactttagga agggataact aacggctatg gagttaagta tgtaaaatag
     1981 cccgcagaaa aatattcacc ttatcaataa ttcgttacgg agaacacgac cgatgcggac
     2041 tctgcagggc tggttgttgc cggtgtttat gttgcctatg gcagtatatg cacaagaggc
     2101 aacggtgaaa gaggtgcatg acgcgccagc ggtgcgtggc agtattatcg ccaatatgct
     2161 gcaggagcat gacaatccgt tcacgctcta tccttatgac accaactacc tcatttacac
     2221 ccaaaccagc gatctgaata aagaagcgat tgccagttac gactgggcgg aaaatgcgcg
     2281 taaggatgaa gtaaagtttc agttgagcct ggcatttccg ctgtggcgtg ggattttagg
     2341 cccgaactcg gtgttgggtg cgtcttatac gcaaaaatcc tggtggcaac tgtccaatag
     2401 cgaagagtct tcaccgtttc gtgaaaccaa ctacgaaccg caattgttcc tcggttttgc
     2461 caccgattac cgttttgcag gttggacgct gcgcgatgtg gagatggggt ataaccacga
     2521 ctctaacggg cgttccgacc cgacctcccg cagctggaac cgcctttata ctcgcctgat
     2581 ggcagaaaac ggtaactggc tggtagaagt gaagccgtgg tatgtggtgg gtaatactga
     2641 cgataacccg gatatcacca aatatatggg ttactaccag cttaaaatcg gctatcacct
     2701 cggtgatgcg gtgctcagtg cgaaaggaca gtacaactgg aacaccggct acggcggcgc
     2761 ggagttaggc ttaagttacc cgatcaccaa acatgtgcgc ctttatactc aggtttacag
     2821 cggctatggc gaatcgctca tcgactataa cttcaaccag acccgtgtcg gtgtgggggt
     2881 tatgctaaac gatttgtttt gatgaacggt tgagtggttg gcaaatctgg aatccagcat
     2941 ccaggattac cctctcagag actaaaagca ttgcagtttc tcgcgcaggc gctgaaaata
     3001 gcgcctgttt ttatttcagg caatcggggt gaatgtggcg caggcggaag tgttgaatct
     3061 ggagtccgga gctaaacagg ttttacaaga aacctttggc taccaacagt ttcgccccgg
     3121 ccaggaagaa attatcgaca ctgtgctttc cggtcgcgat tgcctggtcg tcatgcccac
     3181 cggtggcgga aaatcccttt gctatcaaat tcctgcctta ttgctaaacg gccttaccgt
     3241 ggttgtttca ccgctgattt cgttgatgaa agatcaggtg gatcaactgc aagccaacgg
     3301 cgtggcggcg gcgtgcctta actcgacgca aacccgcgaa cagcaacttg aagtgatgac
     3361 aggctgccgc accgggcaaa ttcgtctgct ttatatcgcc ccggaacgcc tgatgctgga
     3421 taactttctt gagcatctgg cgcactggaa tccggtgtta ttagccgttg atgaagcgca
     3481 ctgtatctcc caatggggcc acgatttccg cccggaatat gccgcgctcg gtcagttgcg
     3541 ccagcggttc ccgacgctgc cgtttatggc gctgaccgcc acagccgacg acaccacgcg
     3601 ccaggatatc gtgcgcctgc tggggctgaa cgatccgctg attcaaatca gcagttttga
     3661 ccgtccgaat attcgctaca tgctgatgga gaagttcaaa ccgctcgatc agttgatgcg
     3721 ctacgtgcag gaacagcgcg gtaagtccgg cattatctac tgcaacagcc gggcgaaagt
     3781 agaagacacc gctgcgcgcc tgcaaagcaa gggtattagc gcggcggcct atcatgccgg
     3841 gctggaaaat aatgtccgcg ccgacgtgca ggagaaattc cagcgcgatg acctgcaaat
     3901 tgtggtggcg acggtggcgt tcggcatggg catcaataaa ccaaacgttc gcttcgtggt
     3961 ccactttgat attccgcgca atatcgaatc ctattatcag gaaaccggtc gcgccgggcg
     4021 tgatggcctg cccgcggaag cgatgctgtt ttacgatccg gccgatatgg cgtggctgcg
     4081 ccgttgtctg gaagagaagc cgcaggggca gttgcaggat atcgaacgtc ataaactcaa
     4141 tgcgatgggc gcttttgccg aagcgcaaac ttgccgtcgt ctggtactgc tgaactattt
     4201 tagcgaaggg cgtcaggagc cgtgcgggaa ctgcgatatc tgcctcgatc cgccgaaaca
     4261 gtacgacggt tcaaccgatg ctcagattgc cctttccacc attggtcgtg tgaatcagcg
     4321 ttttggtatg ggctatgtgg tggaagtgat tcgcggcgct aataaccagc gtatccgcga
     4381 ctatggtcat gacaaactaa aagtctacgg tatgggccgt gataaaagtc atgaacattg
     4441 ggtgagcgtg atccgccagt tgattcacct cggcctggtg acgcaaaata ttgcccagca
     4501 ttctgccctc caactgacag aggccgcgcg tccggtgctg cgcggcgaat cctctttgca
     4561 acttgccgtg ccgcgtatcg tggcgctcaa accgaaagcg atgcagaaat cgttcggcgg
     4621 caactatgat cgcaaactgt tcgccaaatt acgcaaactg cgtaaatcga ttgccgatga
     4681 aagcaatgtc ccgccgtacg tggtgtttaa cgacgcaacg ttgattgaga tggctgaaca
     4741 gatgccgatc accgccagcg aaatgctcag cgttaacggc gttgggatgc gcaagctgga
     4801 acgctttggt aaaccgttta tggcgcttat ccgcgcgcat gttgacggcg acgacgaaga
     4861 gtagtcagcg gcataaaaaa gtgccaggat gaagactccg taaacgtttc ccccacgagt
     4921 caaatgtatg ttgatgttat ttctcaccgt cgccatggtg catattgtgg cgcttatgag
     4981 ccccggtccc gatttctttt ttgtctctca gaccgctgtc agtcgttccc gtaaagaagc
     5041 gatgatgggc gtgctgggca ttacctgcgg tgtaatggtt tgggctggga ttgcgttgct
     5101 tggcctgcat ttgattatcg aaaaaatggc ctggctgcat acgctgatta tggttggcgg
     5161 tggcctgtat ctctgctgga tgggttacca gatgctacgc ggtgcactga aaaaagaggc
     5221 ggtttctgca cctgcgccac aggtcgagct ggcgaaaagt gggcgcagtt tcctgaaagg
     5281 tttactgacc aatctcgcta atccgaaagc gattatctac tttggctcgg tgttctcatt
     5341 gtttgtcggt gataacgttg gcactaccgc gcgctggggc atttttgcgc tgatcattgt
     5401 cgaaacgctg gcgtggttta ccgtcgttgc cagcctgttt gccctgccgc aaatgcgccg
     5461 tggttatcaa cgtctggcga agtggattga tggttttgcc ggggcgttat ttgccggatt
     5521 tggcattcat ttgattattt cgcggtgatg ccagacgcgt cttcagagta agtcggataa
     5581 ggcgtttacg ccgcatccga cattattttt cacgcatgcc tcgccgatgc taacagcgct
     5641 cccaccagca taaacaacga gccgaaaatc ttattcagcg ccttcatctg ctttggtcct
     5701 ttaatccata gagcaatccg ttgagcaagg gtggcgtaac cgatcatcac aataatatcg
     5761 accacaatag tggtgacgcc gagcacgata tactgcatca gttgcggctg ttgcggcatg
     5821 atgaattgcg gaaatagcgc cgccagaaac acaatacttt tgggattggt gagattcaca
     5881 aaaactgcgc gctggaacaa atgtcgacgc gattgagtag aggccagcga tttaaggtca
     5941 attgcaccag cggcgcgcca ctgctggatt cccagccaaa tcaagtaagc cgcgcctgcc
     6001 cacttcaaca cttcaaacgc aatcactgag cgggaaaata gcgtccccaa ccccacgcca
     6061 accagcacaa tatgaatcgc cagtccggtc tgaagcccag caatagacgc caccgcgccg
     6121 cgataaccgt ggttgagcga ggtggtcata gtgttgattg caccagagcc tggcgacagg
     6181 cttaaaatga tcgatgtcag caggtaggca aaccaccatt ctaaggtcat gatgaactcc
     6241 cggtgtgtct atttttgtgc cacaatacgc tactgtcgca gcgttgtgtc aggcacgcta
     6301 aaaaaaacga ttttacgtgg tttaagaggc agattacccg atgtttcagc agcaaaaaga
     6361 ctgggaaaca agagaaaacg cgtttgctgc ttttaccatg ggaccgctga ctgatttctg
     6421 gcgtcagcgt gatgaagcag agtttactgg tgtggatgac attccggtgc gctttgtccg
     6481 ttttcgcgca cagcaccatg accgggtggt agtcatctgc ccggggcgta ttgagagcta
     6541 cgtaaaatat gcggaactgg cctacgattt gttccatttg gggtttgatg tcttaatcat
     6601 cgaccatcgc gggcagggac gttccggtcg cctgttagcc gatccgcatc tcgggcatgt
     6661 taatcgcttt aatgattatg ttgatgatct ggcggcattc tggcaacagg aggtccagcc
     6721 cggtccgtgg cgtaaacgct atatactggc acattcgatg ggcggtgcga tctccacatt
     6781 atttctgcaa cgccatccag gtgtatgtga cgccattgcg ctaactgcgc caatgtttgg
     6841 gatcgtgatt cgtatgccgt catttatggc acggcagatc ctcaactggg ccgaagcgca
     6901 tccacgtttc cgtgatggct atgcaatagg caccgggcgc tggcgcgcgt tgccgtttgc
     6961 tatcaacgta ctgacccaca gcagacagcg atatcgacgt aacttacgct tctatgctga
     7021 tgacccaacg attcgcgtcg gtgggccgac ctaccattgg gtacgcgaaa gtattctggc
     7081 tggcgaacag gtgttagccg gtgcgggtga tgacgccacg ccaacgcttc tcttgcaggc
     7141 tgaagaggaa cgcgtggtgg ataaccgcat gcatgaccgt ttttgtgaac tccgcaccgc
     7201 cgcgggccat cctgtcgaag gaggacggcc gttggtaatt aaaggtgctt accatgagat
     7261 cctttttgaa aaggacgcaa tgcgctcagt cgcgctccac gccatcgttg attttttcaa
     7321 caggcataac tcacccagcg gaaaccgctc tacagaggtt taaatttctt atgtaccagg
     7381 ttgttgcgtc tgatttagat ggcacgttac tttctcccga ccatacgtta tccccttacg
     7441 ccaaagaaac tctgaagctg ctcaccgcgc gcggcatcaa ctttgtgttt gcgaccggtc
     7501 gtcaccacgt tgatgtgggg caaattcgcg ataatctgga gattaagtct tacatgatta
     7561 cctccaatgg tgcgcgcgtt cacgatctgg atggtaatct gatttttgct cataacctgg
     7621 atcgcgacat tgccagcgat ctgtttggcg tagtcaacga caatccggac atcattacta
     7681 acgtttatcg cgacgacgaa tggtttatga atcgccatcg cccggaagag atgcgctttt
     7741 ttaaagaagc ggtgttcaaa tatgcgctgt atgagcctgg attactggag ccggaaggcg
     7801 tcagcaaagt gttcttcacc tgcgattccc atgaacaact gctgccgctg gagcaggcga
     7861 ttaacgctcg ttggggagat cgcgtcaacg tcagtttctc taccttaacc tgtctggaag
     7921 tgatggcggg cggcgtttca aaaggccatg cgctggaagc ggtggcgaag aaactgggct
     7981 acagcctgaa ggattgtatt gcgtttggtg acgggatgaa cgacgccgaa atgctgtcga
     8041 tggcggggaa aggctgcatt atgggcagtg cgcaccagcg tctgaaagac cttcatcccg
     8101 agctggaagt gattggtact aatgccgacg acgcggtgcc gcattatctg cgtaaactct
     8161 atttatcgta atcgttcttt atttggtcag ttgtcaacct gatacttcgc tacaatggat
     8221 actcgttaat caaagagttt tccattgtgg cgctacttat catcaccacg attctgtggg
     8281 ccttctcctt tagcttttat ggcgagtacc ttgcggggca cgtcgatagc tattttgcgg
     8341 tgctggtgcg cgttggcctg gcggcactcg tttttctgcc gtttctgcgt acccgtggca
     8401 atagcctgaa aacagtcggc ctgtatatgc tggtgggcgc gatgcagctt ggcgtgatgt
     8461 atatgctgag tttccgcgct tatctctatc tgacggtttc cgaactgctg ctgttcaccg
     8521 tactgacgcc gctctacatc acgctgattt atgacatcat gagtaagcgc cgtctgcgct
     8581 ggggatatgc ttttagtgcc ttgttggcgg tgattggtgc cgggattatt cgctatgatc
     8641 aggtcaccga ccatttctgg actggcttgt tgctggtgca actctccaat atcacttttg
     8701 ccatcggcat ggtgggttac aaacgcctga tggaaactcg cccgatgccg cagcataacg
     8761 cctttgcgtg gttctatctt ggcgcgttcc tggtggcggt aattgcatgg ttcttgctgg
     8821 gaaatgcgca gaaaatgccg caaaccacgt tgcaatgggg cattctggtg tttcttggcg
     8881 tggtggcttc cgggattggc tactttatgt ggaactacgg cgcgacgcag gtggacgccg
     8941 gaacgctggg aattatgaat aatatgcacg ttccggcagg gctgctggta aacctggcta
     9001 tctggcacca acagccgcac tggccaacgt ttattacagg cgcgctggtg atcctggcct
     9061 cactgtgggt gcatcgtaag tgggtcgctc cgcgctcttc acaaacggca gatgatcgca
     9121 ggcgtgattg cgcgctgagc gaataaacgc ttccgtaact ggctgacgct gctcgccatc
     9181 gcgcacggcg gcgtacagtc ggctccacaa gccttcgccc agggttttgg tcaccaccag
     9241 accctggcgc tcaaaactct ctactaccca atgcggtagc gcggcaatac ccatccgcgc
     9301 ggcaaccatc tgaatcaaca ataaggtgtt atcgacgctt ttcagtgacg ggctgacgcc
     9361 tgccggctga agaaaatgcc gccagacatc cagtcgacta cgctgcaccg gataaatcaa
     9421 cagcgtctcg ctggcgagat cttccggtgt aattcgcgtt ttcgccgcca gtggatggtc
     9481 aggtgctaac accagacgca cttcatagtc gaacatcggc gaataatgca ggccactgcg
     9541 cggcaggata tcggaggtca tcaccagatc cagctcgccc tgttgcaacg cgggctgcgg
     9601 gtcaaacgtc acgcccgatt taaaatccat ctctacctgc ggccagttct tatggaaatt
     9661 ttctaacgcg ggcgtcagcc actgaataca gctatggcac tcaatggcaa tgcgcagacg
     9721 cgtctgctgc ggttcattgc aggcttgcag tgcctggcta atttgcggca gcacctggtt
     9781 tgccagttgc aacaggattt ctccctgcgg tgtaaagcgt agcggctggc tcttacgcac
     9841 aaatagccgg aagccaaggc gttgttccag atcgctaaac tggtgagaca gggcggattg
     9901 cgtctgatgc aacgtcgccg cagcggctgc gagagagccg cagttccgca acgcttgtag
     9961 cgttttcagg tgttttactt cgatcatgaa agtccttcac ttcggcatga ataatttgcg
    10021 cttgaggaat atacagtaac cgccaattat ggatgtgtaa acatctggac ggctaaatac
    10081 taaaaattcc ataaggggca tatcatataa tgacaattct taatcacacc ctcggtttcc
    10141 ctcgcgttgg cctgcgtcgc gagctgaaaa aagcgcaaga aagttattgg gcggggaact
    10201 ccacgcgtga agaactgctg acagtagggc gtgaactgcg tgctcgtcac tgggatcaac
    10261 aaaagcaagc gggtatcgac ctgctgccgg tgggcgattt tgcctggtac gatcatgttc
    10321 tgaccaccag tctgctgctg ggtaatgttc cgccacgtca tcagaacaaa gacggttcgg
    10381 tagatatcga caccctgttc cgtattggtc gtggacgtgc gccgactggc gaacctgcgg
    10441 cggcagcgga aatgaccaaa tggtttaaca ccaactatca ctacatggtg ccggaattcg
    10501 ttaaaggcca acagttcaaa ctgacctgga cgcagctgct ggaggaagtg gacgaggcgc
    10561 tggcgctggg ccacaacgtt aagcctgtgc tgctggggcc ggttacttac ctgtggctgg
    10621 ggaaagtgaa aggtgaacag tttgaccgcc ttagcctgct gaacgacatt ctgccggttt
    10681 atcagcaagt gctggcagaa ctggcgaaac gcggcatcga gtgggtacag attgatgaac
    10741 ccgcgctggt actggaacta ccacaggcgt ggctggacgc atacaaaccc gcttacgacg
    10801 cgctccaggg acaggtgaaa ctgctgctga ccacctattt tgaaggcgta acgccaaatc
    10861 tcgacacgat tactgcgctg cctgttcagg gtctgcatgt tgacctcgta catggtaaag
    10921 atgacgttgc tgaactgcac aagcgcctgc cttctgactg gttgctgtct gcgggtctga
    10981 tcaatggtcg taacgtctgg cgcgccgatc ttaccgagaa atatgcgcaa attaaggaca
    11041 ttgtcggcaa acgtgatttg tgggtggcat cttcctgctc gttgctgcac agccccatcg
    11101 acctgagcgt ggaaacgcgt cttgatgcag aagtgaaaag ctggtttgcc ttcgccctac
    11161 aaaaatgcca tgaactggca ctgctgcgcg atgcgctgaa cagtggtgac acggcagctc
    11221 tggcagagtg gagcgccccg attcaggcac gtcgtcactc tacccgcgta cataatccgg
    11281 cggtagaaaa gcgtctggcg gcgatcaccg cccaggacag ccagcgtgcg aatgtctatg
    11341 aagtgcgtgc cgaagcccag cgtgcgcgtt ttaaactgcc agcgtggccg accaccacga
    11401 ttggttcttt cccgcaaacc acggaaattc gtaccctgcg tctggatttc aaaaagggca
    11461 atctcgacgc caataactac cgcacgggca ttgcggaaca tatcaggcag gccattgttg
    11521 aacaggaacg tttgggactg gatgtgctgg tacatggcga ggccgagcgt aatgacatgg
    11581 tggaatactt tggcgagcac ctcgacgggt ttgtctttac gcaaaacggt tgggtacaga
    11641 gctacggttc ccgctgcgtg aagccaccga ttgttattgg tgacgttagt cgcccggcac
    11701 cgattaccgt ggagtgggcg aagtatgcgc aatcgctgac cgacaaaccg gtgaaaggga
    11761 tgctgacggg gccggtgacc atactctgct ggtcgttccc gcgtgaagat gtcagccgtg
    11821 aaaccatcgc caaacagatt gcgctggcgc tgcgtgatga ggtggccgat ctggaagcgg
    11881 cagggattgg tatcatccag attgacgaac cggcgctgcg cgaaggttta ccactgcgtc
    11941 gtagcgactg ggacgcctat ctccagtggg gcgtagaggc cttccgtatc aacgccgccg
    12001 tggcgaaaga tgacacacaa atccacactc acatgtgtta ttgcgagttc aacgacatca
    12061 tggattccat tgcggcgctg gatgcagacg tcatcaccat cgaaacctcg cgttccgaca
    12121 tggagttgct ggagtcgttt gaagagtttg attatccgaa tgaaatcggt ccaggcgtct
    12181 atgacattca ctcgccaaac gtaccgagcg tggaatggat tgaagccctg ctgaagaaag
    12241 cggcaaaacg cattccggca gagcgcctgt gggtcaaccc agactgtggc ctgaaaacgc
    12301 gcggctggcc agaaacccgc gcggcactgg cgaacatggt gcaggcggcg cagaatttgc
    12361 gtctggggta aatccaaacc gggtggtaat accacccggt cttttctcat tacagcgact
    12421 tcttcccacc atactgctta aaccattcca gcatacgctg ccagccatct ttcgcagatt
    12481 cggcatgata gctcgggcga taatcagcgt tgaatgcatg cccggcatcc gggtagacga
    12541 taatctctgc tttcgcatta gcagcccgca gcgcctggcg catcgtttca acgctctcct
    12601 gcggaatgct gttatcctga ccgccatata agccgagaac cggcgcgtta agatcggttg
    12661 cgatatcaac aggttgtttc ggtgaattca gcgacttatc gcccgtcagt ttgccgtacc
    12721 acgccactgc ggcttttagc tgtggattat gcgcggcata cagccaggtg atacgtccac
    12781 cccagcagaa tccggtgatc attaaacgat gaacatcgcc gccgttgcgg gaagcccaac
    12841 tggcgacatg atcgagatcg gccagcacct gcgagtcagg cacttttgct accagaccgc
    12901 taagcaacgt ggggatatcg gcaaaatcat tcggatcgcc ttcgcggaag taaagttcag
    12961 gtgcgatagc cagatacccc tccagcgcca gacggcgaca aatgtcgcgg atatgttcat
    13021 gcacgccaaa aatttcctgc actacaatga ccactggcag tgggccatcg ctttgctttg
    13081 gtctggcatg gtaagcaggc atgttatccc cttgtgaagg gatagaggtg aagcccgcca
    13141 caatcgcgtc gtccggggtc tgaacgatgg tcgaagcgag aggcgatgca gcaggtgcaa
    13201 atccagattg ttgtgttgtt gccatggtat tctccgtacc cttataaaaa tgttgcgcaa
    13261 tgttaactat agtcagcatg caacaaatca cattgcctga atcggctcat cttttatgca
    13321 gtcctgcaga atgaagagtg atttatgtga tttgaatcac ttttggtggg taaatttatg
    13381 caacgcattt gcgtcatggt gatgagtatc acgaaaaaat gttaaaccct tcggtaaagt
    13441 gtctttttgc ttcttctgac taaaccgatt cacagaggag ttgtatatgt ccaagtctga
    13501 tgtttttcat ctcggcctca ctaaaaacga tttacaaggg gctacgcttg ccatcgtccc
    13561 tggcgacccg gatcgtgtgg aaaagatcgc cgcgctgatg gataagccgg ttaagctggc
    13621 atctcaccgc gaattcacta cctggcgtgc agagctggat ggtaaacctg ttatcgtctg
    13681 ctctaccggt atcggcggcc cgtctacctc tattgctgtt gaagagctgg cacagctggg
    13741 cattcgcacc ttcctgcgta tcggtacaac gggcgctatt cagccgcata ttaatgtggg
    13801 tgatgtcctg gttaccacgg cgtctgtccg tctggatggc gcgagcctgc acttcgcacc
    13861 gctggaattc ccggctgtcg ctgatttcga atgtacgact gcgctggttg aagctgcgaa
    13921 atccattggc gcgacaactc acgttggcgt gacagcttct tctgatacct tctacccagg
    13981 ccaggaacgt tacgatactt actctggtcg cgtagttcgt cactttaaag gttctatgga
    14041 agagtggcag gcgatgggcg taatgaacta tgaaatggaa tctgcaaccc tgctgaccat
    14101 gtgtgcaagt cagggcctgc gtgccggtat ggtagcgggt gttatcgtta accgcaccca
    14161 gcaagagatc ccgaatgctg agacgatgaa acaaactgaa agccaagcgg tgaaaatcgt
    14221 ggtggaagcg gcgcgtcgtc tgctgtaatt ctcttctcct gtctgaaggc cgacgcgttc
    14281 ggccttttgt atttttgcgt agcgcctcgc aggaaatgcc tttccaactg gacgtttgta
    14341 cagcacaatt ctattttgtg cgggtaagtt gttgcgtcag gaggcgttgt ggatttctca
    14401 atcatggttt acgcagttat tgcgttggtg ggtgtggcaa ttggctggct gtttgccagt
    14461 tatcaacatg cgcagcaaaa agccgagcaa ttagctgaac gtgaagagat ggtcgcggag
    14521 ttaagcgcgg caaaacaaca aattacccaa agcgagcact ggcgtgcaga gtgcgagtta
    14581 ctcaataacg aagtgcgcag cctgcaaagt attaacacct ctctggaggc cgatctgcgt
    14641 gaagtaacca cgcggatgga agccgcacag caacatgctg acgataaaat tcgccagatg
    14701 attaacagcg agcagcgcct cagtgagcag tttgaaaacc tcgccaaccg tatttttgag
    14761 cacagcaatc gccgggttga tgagcaaaac cgtcagagtc tgaacagcct gttgtcgccg
    14821 ctacgtgaac aactggacgg tttccgccgt caggttcagg acagcttcgg taaagaagca
    14881 caagaacgcc ataccctgac ccacgaaatt cgcaatctcc agcaactcaa cgcgcaaatg
    14941 gcccaggaag cgatcaacct gacgcgcgcg ctgaaaggcg acaataaaac ccagggcaac
    15001 tggggcgagg tagtattgac gcgggtgctg gaggcttccg gtctgcgtga agggtatgaa
    15061 tatgaaaccc aggtcagcat cgaaaatgac gcccgctcgc ggatgcagcc ggatgtcatc
    15121 gtgcggctgc cgcagggaaa agatgtggtg atcgacgcca aaatgacgct ggtcgcctat
    15181 gaacgctatt ttaacgccga agacgactac acccgcgaaa gcgcgctaca ggaacatatc
    15241 gcgtcggtgc gtaaccatat ccgtttgctg ggacgcaaag attatcaaca gctgccgggg
    15301 ctgcgaactc tggattacgt gctgatgttt attcccgttg aacccgcttt tttactggcg
    15361 cttgaccgcc agccggagct gatcaccgaa gcgttgaaaa acaatatcat gctggttagc
    15421 ccgactacgc tgctggtggc gttgcgtact attgccaacc tgtggcgtta tgagcatcaa
    15481 agccgcaacg cccagcaaat cgccgatcgt gccagcaagc tgtacgacaa gatgcgtttg
    15541 ttcatcgatg acatgtccgc gattggtcaa agtctcgaca aagcgcagga taactaccgc
    15601 caggcaatga aaaaactctc ttcaggtcgc ggaaatgtgc tggcgcaggc agaagcgttt
    15661 cgcggtttag gagtagaaat taaacgcgag attaatccgg atttggctga acaggcggtg
    15721 agccaggatg aagagtatcg acttcggtcg gttccggagc agccgaatga tgaagcttat
    15781 caacgcgatg atgaatataa tcagcagtcg cgctagccca ttaggggtag ttaagccggg
    15841 tagaaatcta gggcatcgac gcccaatctg ttacacttct ggaacaattt tttgatgagc
    15901 aggcattgag atggtggata agtcacaaga aacgacgcac tttggttttc agaccgtcgc
    15961 gaaggaacaa aaagcggata tggtcgccca cgttttccat tccgtggcat caaaatacga
    16021 tgtcatgaat gatttgatgt catttggtat tcatcgtttg tggaagcgat tcacgattga
    16081 ttgcagcggc gtacgccgtg ggcagaccgt gctggatctg gctggtggca ccggcgacct
    16141 gacagcgaaa ttctcccgcc tggtcggaga aactggcaaa gtggtccttg ctgatatcaa
    16201 tgaatccatg ctcaaaatgg gacgcgagaa gctgcgtaat atcggtgtga ttggcaacgt
    16261 tgagtatgtt caggcgaacg ctgaggcgct gccgttcccg gataacacct ttgattgcat
    16321 caccatttcg tttggtctgc gtaacgtcac cgacaaagat aaagcactgc gttcaatgta
    16381 tcgcgtgctg aaacccggcg gccgcctgct ggtgcttgag ttctcgaagc caattatcga
    16441 gccgctgagc aaagcctatg atgcatactc cttccatgtg ctgccgcgta ttggctcact
    16501 ggtcgcgaac gacgccgaca gctaccgtta tctggcagaa tccatccgta tgcatcccga
    16561 tcaggatacc ctgaaagcca tgatgcagga tgccggattc gaaagtgtcg actactacaa
    16621 tctgacggcg ggggttgtgg cgctgcatcg tggttataag ttctgacagg agaccggaaa
    16681 tgccttttaa acctttagtg acggcaggaa ttgaaagtct gctcaacacc ttcctgtatc
    16741 gctcacccgc gctgaaaaca gcccgctcgc gtctgctggg taaagtattg cgcgtggagg
    16801 taaaaggctt ttcgacgtca ttgattctgg tgttcagcga acgccaggtt gatgtactgg
    16861 gcgaatgggc aggcgatgct gactgcaccg ttatcgccta cgccagtgtg ttgccgaaac
    16921 ttcgcgatcg ccagcagctt accgcactga ttcgcagtgg tgagctggaa gtgcagggcg
    16981 atattcaggt ggtgcaaaac ttcgttgcgc tggcagatct ggcagagttc gaccctgcgg
    17041 aactgctggc cccttatacc ggtgatatcg ccgctgaagg aatcagcaaa gccatgcgcg
    17101 gaggcgcaaa gttcctgcat cacggcatta agcgccagca acgttatgtg gcggaagcca
    17161 ttactgaaga gtggcgtatg gcacccggtc cgcttgaagt ggcctggttt gcggaagaga
    17221 cggctgccgt cgagcgtgct gttgatgccc tgaccaaacg gctggaaaaa ctggaggcta
    17281 aatgacgcca ggtgaagtac ggcgcctata tttcatcatt cgcacttttt taagctacgg
    17341 acttgatgaa ctgatcccca aaatgcgtat caccctgccg ctacggctat ggcgatactc
    17401 attattctgg atgccaaatc ggcataaaga caaactttta ggtgagcgac tacgactggc
    17461 cctgcaagaa ctggggccgg tttggatcaa gttcgggcaa atgttatcaa cccgccgcga
    17521 tctttttcca ccgcatattg ccgatcagct ggcgttattg caggacaaag ttgctccgtt
    17581 tgatggcaag ctggcgaagc agcagattga agctgcaatg ggcggcttgc cggtagaagc
    17641 gtggtttgac gattttgaaa tcaagccgct ggcttctgct tctatcgccc aggttcatac
    17701 cgcgcgattg aaatcgaatg gtaaagaggt ggtgattaaa gtcatccgcc cggatatttt
    17761 gccggttatt aaagcggatc tgaaacttat ctaccgtctg gctcgctggg tgccgcgttt
    17821 gctgccggat ggtcgccgtc tgcgcccaac cgaagtggtg cgcgagtacg aaaagacatt
    17881 gattgatgaa ctgaatttgc tgcgggaatc tgccaacgcc attcagcttc ggcgcaattt
    17941 tgaagacagc ccgatgctct acatcccgga agtttaccct gactattgta gtgaagggat
    18001 gatggtgatg gagcgcattt acggcattcc ggtgtctgat gttgcggcgc tggagaaaaa
    18061 cggcaccaac atgaaattgc tggcggaacg cggcgtgcag gtgttcttca ctcaggtctt
    18121 tcgcgacagc tttttccatg ccgatatgca ccctggcaac atcttcgtaa gctatgaaca
    18181 cccggaaaac ccgaaatata tcggcattga ttgcgggatt gttggctcgc taaacaaaga
    18241 agataaacgc tatctggcag aaaactttat cgccttcttt aatcgcgact atcgcaaagt
    18301 ggcagagcta cacgtcgatt ctggctgggt gccaccagat accaacgttg aagagttcga
    18361 atttgccatt cgtacggtct gtgaacctat ctttgagaaa ccgctggccg aaatttcgtt
    18421 tggacatgta ctgttaaatc tgtttaatac ggcgcgtcgc ttcaatatgg aagtgcagcc
    18481 gcaactggtg ttactccaga aaaccctgct ctacgtcgaa ggggtaggac gccagcttta
    18541 tccgcaactc gatttatgga aaacggcgaa gcctttcctg gagtcgtgga ttaaagatca
    18601 ggtcggtatt cctgcgctgg tgagagcatt taaagaaaaa gcgccgttct gggtcgaaaa
    18661 aatgccagaa ctgcctgaac tggtttacga cagtttgcgc cagggcaagt atttacagca
    18721 cagtgttgat aagattgccc gcgagcttca gtcaaatcat gtacgtcagg gacaatcgcg
    18781 ttattttctc ggaattggcg ctacgttagt attaagtggc acattcttgt tggtcagccg
    18841 acctgagtgg gggctgatgc ccggctggtt aatggcaggt ggtctgatcg cctggtttgt
    18901 cggttggcgc aaaacacgct gattttttca tcgctcaagg cgggccgtgt aacgtataat
    18961 gcggctttgt ttaatcatca tctaccacag aggaacatgt atgggtggta tcagtatttg
    19021 gcagttattg attattgccg tcatcgttgt actgcttttt ggcaccaaaa agctcggctc
    19081 catcggttcc gatcttggtg cgtcgatcaa aggctttaaa aaagcaatga gcgatgatga
    19141 accaaagcag gataaaacca gtcaggatgc tgattttact gcgaaaacta tcgccgataa
    19201 gcaggcggat acgaatcagg aacaggctaa aacagaagac gcgaagcgcc acgataaaga
    19261 gcaggtgtaa tccgtgtttg atatcggttt tagcgaactg ctattggtgt tcatcatcgg
    19321 cctcgtcgtt ctggggccgc aacgactgcc tgtggcggta aaaacggtag cgggctggat
    19381 tcgcgcgttg cgttcactgg cgacaacggt gcagaacgaa ctgacccagg agttaaaact
    19441 ccaggagttt caggacagtc tgaaaaaggt tgaaaaggcg agcctcacta acctgacgcc
    19501 cgaactgaaa gcgtcgatgg atgaactacg ccaggccgcg gagtcgatga agcgttccta
    19561 cgttgcaaac gatcctgaaa aggcgagcga tgaagcgcac accatccata acccggtggt
    19621 gaaagataat gaagctgcgc atgagggcgt aacgcctgcc gctgcacaaa cgcaggccag
    19681 ttcgccggaa cagaagccag aaaccacgcc agagccggtg gtaaaacctg ctgcggacgc
    19741 tgaaccgaaa accgctgcac cttccccttc gtcgagtgat aaaccgtaaa catgtctgta
    19801 gaagatactc aaccgcttat cacgcatcta attgagctgc gtaagcgtct gctgaactgc
    19861 attatcgcgg tgatcgtgat attcctgtgt ctggtctatt tcgccaatga catctatcac
    19921 ctggtatccg cgccattgat caagcagttg ccgcaaggtt caacgatgat cgccaccgac
    19981 gtggcctcgc cgttctttac gccgatcaag ctgaccttta tggtgtcgct gattctgtca
    20041 gcgccggtga ttctctatca ggtgtgggca tttatcgccc cagcgctgta taagcatgaa
    20101 cgtcgcctgg tggtgccgct gctggtttcc agctctctgc tgttttatat cggcatggcg
    20161 ttcgcctact ttgtggtctt tccgctggca tttggcttcc ttgccaatac cgcgccggaa
    20221 ggggtacagg tatccaccga catcgccagc tatttaagct tcgttatggc gttgtttatg
    20281 gcgtttggtg tctcctttga agtgcctgtg gcaattgtgc tgctgtgctg gatggggatt
    20341 acctcgccag aagacttacg caaaaaacgc ccgtatgtgc tggttggtgc attcgttgtc
    20401 gggatgctgc tgacaccgcc ggatgtcttc tcgcaaacgc tgttggcgat cccgatgtac
    20461 tgtctgtttg aaatcggtgt cttcttctca cgcttttacg ttggtaaagg gcgaaatcgg
    20521 gaagaggaaa acgacgctga agcagaaagc gaaaaaactg aagaataaat tcaaccgccc
    20581 gtcagggcgg ttgtcatatg gagtacagga tgtttgatat cggcgttaat ttgaccagtt
    20641 cgcaatttgc gaaagaccgt gatgatgttg tagcgcgcgc ttttgacgcg ggagttaatg
    20701 ggctactcat caccggcact aacctgcgtg aaagccagca ggcgcaaaag ctggcgcgtc
    20761 agtattcgtc ctgttggtca acggcgggcg tacatcctca cgacagcagc cagtggcaag
    20821 ctgcgactga agaagcgatt attgagctgg ccgcgcagcc agaagtggtg gcgattggtg
    20881 aatgtggtct cgactttaac cgcaactttt cgacgccgga agagcaggaa cgcgcttttg
    20941 ttgcccagct acgcattgcc gcagatttaa acatgccggt atttatgcac tgtcgcgatg
    21001 cccacgagcg gtttatgaca ttgctggagc cgtggctgga taaactgcct ggtgcggttc
    21061 ttcattgctt taccggcaca cgcgaagaga tgcaggcgtg cgtggcgcgt ggaatttata
    21121 tcggcattac cggttgggtt tgcgatgaac gacgcgggct ggagctgcgg gaattgttgc
    21181 cgttgattcc ggcggagaaa ttgctgatcg aaactgatgc gccgtatctg ctcccgcgcg
    21241 atctcacgcc aaagccatca tcccggcgca acgagccagc ccatctgccc catattttgc
    21301 aacgtattgc gcactggcgt ggagaagatg ccgcatggct ggctgccacc acggatgcca
    21361 atgtcaaaac actgtttggg attgcgtttt agagtttgcg gaactcggtg ttcttcacac
    21421 tgtgcttaat ctctttatta ataagattaa gcaatagcat ggagcgagcc tcaccatcgg
    21481 gttcggtgaa aatggcctga aagccttcga acgcgccttc ggtaataatc accttatcac
    21541 ccggataagg ggttgccgga tcgacaatgt ctttcggttt atataccgat agctgatgaa
    21601 tcaccgccga tgggactatc gctggcgacg cgccaaagcg cacgaagtgg ctgacaccgc
    21661 gggtcgcgtt gatagtcgtg gtatgaatca cttctggatc aaattcgaca aacaggtagt
    21721 tgggaaacaa tggctcactg actgcagtac gttttccacg cacgattttt tccagggtga
    21781 tcatcggtgc caggcaattc acagcctgtc tttcgaggtg ttcctgggca cgttgaagtt
    21841 gcccgcgctt gcagtacagt aaataccagg attgcataat gactcttatc cgtttaatcg
    21901 gggcgcaagg atagcaaaag ctttacgcta agttaattat attccccggt ttgcgttata
    21961 ccgtcagagt tcacgctaat ttaacaaatt tacagcatcg caaagatgaa cgccgtataa
    22021 tgggcgtaga ttaagaggct acaatggacg ccatgaaata taacgattta cgcgacttcc
    22081 tgacactgct tgaacagcag ggtgagctaa aacgtatcac gctcccggtg gatccgcatc
    22141 tggaaatcac tgaaattgct gaccgcactt tgcgtgccgg tgggcctgcg ctgttgttcg
    22201 aaaaccctaa aggctactca atgccggtgc tgtgcaacct gttcggtacg ccaaagcgcg
    22261 tggcaatggg catggggcag gaagatgttt cggcgctacg tgaagttggt aaattattgg
    22321 cgtttctgaa agagccggag ccgccaaaag gtttccgcga cctgtttgat aaactgccgc
    22381 agtttaagca agtattgaac atgccgacaa agcggctgcg tggtgcgccc tgccaacaaa
    22441 aaatcgtctc tggcgatgac gtcgatctca atcgcattcc cattatgacc tgctggccgg
    22501 aagatgccgc gccgctgatt acctgggggc tgacagtgac gcgcggcccg cataaagagc
    22561 ggcagaatct gggcatttat cgccagcagc tgattggtaa aaacaaactg attatgcgct
    22621 ggctgtcgca tcgcggcggc gcgctggatt atcaggagtg gtgtgcggcg catccgggcg
    22681 aacgtttccc ggtttctgtg gcgctgggtg ccgatcccgc cacgattctc ggtgcagtca
    22741 cccccgttcc ggatacactt tcagagtatg cgtttgccgg attgctgcgc ggtaccaaga
    22801 ccgaagtggt gaagtgtatc tccaatgacc ttgaagtgcc cgccagtgcg gagattgtgc
    22861 tggaagggta tatcgaacaa ggtgaaacgg cgccggaagg gccgtatggc gaccacaccg
    22921 gttactataa cgaagtcgat agtttccctg tctttactgt gacgcatatt acccagcgtg
    22981 aagatgcgat ttaccattcc acctataccg ggcgtccgcc tgatgaacct gcggtactgg
    23041 gcgtggcgtt gaacgaagtg tttgtgccga ttctgcaaaa acagttcccg gaaattgtcg
    23101 atttttacct gccaccggaa ggctgttctt atcgtctggc ggtagtgacg atcaaaaaac
    23161 agtacgccgg acacgcgaag cgcgtcatga tgggcgtctg gtcgttctta cgccagttta
    23221 tgtacactaa atttgtgatc gtttgcgatg atgacgtcaa cgcacgcgac tggaacgatg
    23281 tgatttgggc gattaccacc cgtatggacc cggcgcggga tactgttctg gtagaaaata
    23341 cgcctattga ttatctggat tttgcctcgc ctgtctccgg gctgggttca aaaatggggc
    23401 tggatgccac gaataaatgg ccgggggaaa cccagcgtga atggggacgt cccatcaaaa
    23461 aagatccaga tgttgtcgcg catattgacg ccatctggga tgaactggct atttttaaca
    23521 acggtaaaag cgcctgatgc gcgtttgttt tgccctattt atcgatccga cagagaaagc
    23581 gcatgacaac cttaagctgt aaagtgacct cggtagaagc tatcacggat accgtatatc
    23641 gtgtccgcat cgtgccagat gcggcctttt cttttcgcgc tggtcagtat ttgatggtag
    23701 tgatggatga gcgcgacaaa cgtccgttct caatggcttc gacgccggat gaaaaagggt
    23761 ttatcgagct gcatattggc gcttctgaaa tcaaccttta cgcgaaagca gtcatggacc
    23821 gcatcctcaa agatcatcaa atcgtggtcg atattcccca cggagaagcg tggctgcgcg
    23881 atgatgaaga gcgtccgatg attttgattg cgggcggcac cgggttctct tatgcccgct
    23941 cgattttgct gacagcgttg gcgcgtaacc caaaccgtga tatcaccatt tactggggcg
    24001 ggcgtgaaga gcagcatctg tatgatctct gcgagcttga ggcgctttcg ttgaagcatc
    24061 ctggtctgca agtggtgccg gtggttgaac aaccggaagc gggctggcgt gggcgtactg
    24121 gcaccgtgtt aacggcggta ttgcaggatc acggtacgct ggcagagcat gatatctata
    24181 ttgccggacg ttttgagatg gcgaaaattg cccgcgatct gttttgcagt gagcgtaatg
    24241 cgcgggaaga tcgcctgttt ggcgatgcgt ttgcatttat ctgagatata aaaaaacccg
    24301 cccctgacag gcgggaagaa cggcaactaa aactttattc agtggcattt agatctatgt
    24361 cgcatccggc attcggcaca cga
//
LOCUS       DAZFXT010000069        24193 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.069, whole genome shotgun sequence.
ACCESSION   DAZFXT010000069 DAZFXT010000000
VERSION     DAZFXT010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.069"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..90
                     /locus_tag="ACHKW0_002942"
     CDS             <1..90
                     /locus_tag="ACHKW0_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475743.1"
                     /translation="LEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            246..1781
                     /gene="gadC"
                     /locus_tag="ACHKW0_002943"
     CDS             246..1781
                     /gene="gadC"
                     /locus_tag="ACHKW0_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HFO7475744.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1912..3231
                     /gene="digH"
                     /locus_tag="ACHKW0_002944"
     CDS             1912..3231
                     /gene="digH"
                     /locus_tag="ACHKW0_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 10 glycosylhydrolase"
                     /protein_id="HFO7475745.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISTPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSLLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3608..4990
                     /gene="dosC"
                     /locus_tag="ACHKW0_002945"
     CDS             3608..4990
                     /gene="dosC"
                     /locus_tag="ACHKW0_002945"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HFO7475746.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5015..7414
                     /gene="dosP"
                     /locus_tag="ACHKW0_002946"
                     /gene_synonym="pdeO"
     CDS             5015..7414
                     /gene="dosP"
                     /locus_tag="ACHKW0_002946"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase
                     DosP"
                     /protein_id="HFO7475747.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            7672..8253
                     /gene="ddpX"
                     /locus_tag="ACHKW0_002947"
     CDS             7672..8253
                     /gene="ddpX"
                     /locus_tag="ACHKW0_002947"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HFO7475748.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8267..9817
                     /gene="ddpA"
                     /locus_tag="ACHKW0_002948"
     CDS             8267..9817
                     /gene="ddpA"
                     /locus_tag="ACHKW0_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HFO7475749.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVESDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            9819..10841
                     /gene="ddpB"
                     /locus_tag="ACHKW0_002949"
     CDS             9819..10841
                     /gene="ddpB"
                     /locus_tag="ACHKW0_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7475750.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGYLGTSIRTGRPVLEELRIFFPATLE
                     LAFGALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            10838..11734
                     /gene="ddpC"
                     /locus_tag="ACHKW0_002950"
     CDS             10838..11734
                     /gene="ddpC"
                     /locus_tag="ACHKW0_002950"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HFO7475751.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            11731..12717
                     /gene="ddpD"
                     /locus_tag="ACHKW0_002951"
     CDS             11731..12717
                     /gene="ddpD"
                     /locus_tag="ACHKW0_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7475752.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLQTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQLLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDSVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            12710..13636
                     /gene="ddpF"
                     /locus_tag="ACHKW0_002952"
     CDS             12710..13636
                     /gene="ddpF"
                     /locus_tag="ACHKW0_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HFO7475753.1"
                     /translation="MSDTLLTLRDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATSGCEVRQSLAIREDGRELRCWRAL"
     gene            complement(13692..14123)
                     /gene="osmC"
                     /locus_tag="ACHKW0_002953"
     CDS             complement(13692..14123)
                     /gene="osmC"
                     /locus_tag="ACHKW0_002953"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HFO7475754.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
     gene            14469..14684
                     /gene="bdm"
                     /locus_tag="ACHKW0_002954"
     CDS             14469..14684
                     /gene="bdm"
                     /locus_tag="ACHKW0_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HFO7475755.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            14786..14923
                     /gene="sra"
                     /locus_tag="ACHKW0_002955"
     CDS             14786..14923
                     /gene="sra"
                     /locus_tag="ACHKW0_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HFO7475756.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            15080..16777
                     /gene="maeA"
                     /locus_tag="ACHKW0_002956"
     CDS             15080..16777
                     /gene="maeA"
                     /locus_tag="ACHKW0_002956"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7475757.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRMEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            16911..17921
                     /gene="adhP"
                     /locus_tag="ACHKW0_002957"
     CDS             16911..17921
                     /gene="adhP"
                     /locus_tag="ACHKW0_002957"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HFO7475758.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            18068..18352
                     /gene="yddM"
                     /locus_tag="ACHKW0_002958"
     CDS             18068..18352
                     /gene="yddM"
                     /locus_tag="ACHKW0_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HFO7475759.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(18403..19056)
                     /gene="fdnI"
                     /locus_tag="ACHKW0_002959"
     CDS             complement(18403..19056)
                     /gene="fdnI"
                     /locus_tag="ACHKW0_002959"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HFO7475760.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIIEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIILRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(19049..19933)
                     /gene="fdnH"
                     /locus_tag="ACHKW0_002960"
     CDS             complement(19049..19933)
                     /gene="fdnH"
                     /locus_tag="ACHKW0_002960"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HFO7475761.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(19946..22993)
                     /gene="fdnG"
                     /locus_tag="ACHKW0_002961"
     CDS             complement(19946..22993)
                     /gene="fdnG"
                     /locus_tag="ACHKW0_002961"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(22406..22408),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7475762.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKEHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLAGQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLLWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKLLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            23225..24106
                     /gene="yddG"
                     /locus_tag="ACHKW0_002962"
     CDS             23225..24106
                     /gene="yddG"
                     /locus_tag="ACHKW0_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HFO7475763.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         5932 a         6152 c         6107 g         6002 t
ORIGIN      
        1 ctggaagact acaaagcctc cctgaaatat ctcagcgatc acccgaaact gcagggtatt
       61 gcccagcaga acagctttaa acatacctga taacgtttaa cggtaacggt gtcccgaaac
      121 gaacccgttt cgggacaatt tccaaagtct gttcactggc attagcaacg gaaaatattg
      181 ttctgaatac gcttcagaac aaaacaggtg cggttccgac aggaataccg ttttaggggg
      241 ataatatggc tacatcagta cagacaggta aagctaagca gctcacatta ctcggattct
      301 ttgccataac ggcatcgatg gtaatggctg tttatgaata ccctaccttc gcaacatcgg
      361 gcttttcatt agtcttcttc ctgctattag gcgggatttt atggtttatt cccgtgggac
      421 tttgtgctgc ggaaatggcc accgtcgacg gctgggaaga aggtggtgtc ttcgcctggg
      481 tatcaaatac tctggggccg agatggggat ttgcagcgat ctcatttggc tatctgcaaa
      541 tcgccattgg ttttattccg atgctctatt tcgtgttagg ggcactctcc tacatcctga
      601 aatggccagc gctgaatgaa gaccccatta ccaaaactat tgcagcactc atcattcttt
      661 gggcgctggc attaacgcag tttggtggca cgaaatacac ggcgcgaatt gctaaagttg
      721 gcttcttcgc cggtatcctg ttacctgcat ttattttgat cgcattagcg gctatttatc
      781 tgcactccgg tgcccccgtt gctatcgaaa tggattcgaa gaccttcttc cctgacttct
      841 ctaaagtggg cacactggtt gtatttgttg ccttcatttt gagttatatg ggcgtagaag
      901 cttccgcaac tcacgtcaat gaaatgagca accccgggcg cgactatcca ctggctatgt
      961 tactgctgat ggtggcggca atctgcttaa gctctgttgg cggtttatct attgcgatgg
     1021 tcattccggg taatgaaatc aacctctccg caggtgtaat gcaaaccttt accgttctga
     1081 tgtcccatgt ggcaccggaa attgagtgga cggttcgcgt gatctccgca ctgctgttgc
     1141 tgggtgttct ggcggaaatc gcctcctgga ttgttggtcc ttctcgcggg atgtatgtca
     1201 cagcgcagaa aaacctgctg ccagcggcat tcgctaaaat gaacaaaaat ggcgtaccgg
     1261 taacgctggt catttcgcag ctggtgatta cttctatcgc gttgatcatc ctcaccaata
     1321 ccggtggcgg taacaacatg tccttcctga tcgcactggc gctgacggtg gtgatttatc
     1381 tgtgtgctta tttcatgctg tttattggct acatagtgtt ggttcttaaa catcctgact
     1441 taaaacgcac atttaatatc cctggtggta aaggggtgaa attggtcgtg gcaattgtcg
     1501 gtctgctgac ttcaattatg gcgtttattg tttccttcct gccgccggat aacatccagg
     1561 gtgattctac cgatatgtat gttgaattac tggttgttag tttcctggtg gtacttgccc
     1621 tgccctttat tctctatgct gttcatgatc gtaaaggcaa agcgaatacc ggcgtcactc
     1681 tggagccaat caacagtcag aacgcaccaa aaggtcactt cttcctgcac ccgcgtgcac
     1741 gttcaccaca ctatattgtg atgaatgaca agaaacacta accatgtctg aatggttata
     1801 agacaaggga gcgataagtc atcgctccct ttttcgtgct tgctgtcgta ttgacttaac
     1861 cgggataagt acgagaatga gcgcacatct gtttaccgga aaccagcaca tatggatatc
     1921 tgctcccgaa acaagaaatt aacgattaga agaccagcga tactagttgc actggcactt
     1981 ttactgtgta gttgtaaaag cacgcctcca gagtccatgg tgacaccacc agcaggttca
     2041 aagccaccag ccacgacgca acaatcgtca caaccgatgc gtggcatctg gctggccacg
     2101 gtttctcggc tcgactggcc accggtttcc tcggttaaca ttagtacccc caccagccgg
     2161 gcccgtgtac aacaacaggc gatgatcgac aaactggatc atctgcaacg tctcggcata
     2221 aacacggtct ttttccaggt caagccggac ggtaccgccc tgtggccatc gaaaattttg
     2281 ccgtggtccg atcttatgac cggtaagatt ggtgaaaatc cgggttacga tccgctgcaa
     2341 tttatgctcg acgaagccca caagcgtggg atgaaagtac acgcctggtt taacccctat
     2401 cgcgtatcgg ttaatacgaa gcccggtact atcagggaac tgaatagcac tctgtctcaa
     2461 caaccggcga gcgtctatgt gcaacaccgc gactggatca gaacgtctgg cgatcgcttt
     2521 gtcctcgacc cgggcatccc tgaggttcag gactggatca catcaatagt cgccgaagtg
     2581 gtttcccgct atccggtaga tggcgtgcag tttgacgact atttctatac ggagtcaccg
     2641 ggttcactgc taaatgataa cgaaacgtac cgtaaatacg gaggcgcatt tgcgtcaaaa
     2701 gcagactggc ggcgcaacaa tactcagcag ttaattgcaa aggtatcgca caccattaaa
     2761 agcattaagc cgggagtcga atttggtgtt agcccggcag gcgtgtggcg taaccgatca
     2821 cacgatccgc tcggttccga tacccgaggc gcggcagcct atgacgaatc ctacgctgac
     2881 acccgtcgat gggtggaaca aggattgctg gattacattg ctccccaaat ttactggccg
     2941 ttctcacgga gtgccgcgcg ttatgacgtg ttggcaaaat ggtgggcgga tgtcgttaaa
     3001 ccgaccagga cccgcctgta tatcggtatc gccttctata aagtgggtga accttcaaag
     3061 atagagccag actggatgat taacggcggc gtaccggaac tgaaaaagca gctcgatctt
     3121 aacgatgcgg taccagaaat tagcggcacc atcttgttcc gtgaggacta tctgaataaa
     3181 ccgcagactc aacaagcggt cagctatctg caaagtcgtt ggggcagtta aactcccatg
     3241 cttactttgt aacaagccgg tgctttaccc accggcgtgt tacaccttgt gaaatatccc
     3301 actagtaatc atgcttacat aagtcaaatt aaccacgcaa ttaaccacac ttagttaaac
     3361 agctataaat ccctaataaa aatgtatgaa tatgaaaaac catacgatat tcgctgtcac
     3421 cgaaaaataa ttacgatggg ctatactctt ctgaagaaca gtagcgtaga ttgaaactgt
     3481 cctaagtcgc ttatttcttt tcagtatatc ttcatatttc aggagaatat ggtgcagtta
     3541 gtcgatctcg cacgttgcgt ttcgttttga ttccgtctga tttcccagcc ttataagggt
     3601 gtgaattatg gagatgtatt ttaaaagaat gaaagatgag tggaccggac ttgtcgaaca
     3661 ggcagatcct ctcattcgtg ctaaagccgc agaaattgcc cttgcgcatg ctcattatct
     3721 gagtatcgag ttttatcgaa ttgtccgcat cgacccgcat gccgaagaat tcttgagtaa
     3781 tgaacaagtt gagcggcagt tgaaaagtgc gatggaacgc tggattatta acgtgctttc
     3841 tgcccaggtt gacgatgtcg aaaggctaat acaaatccag cataccgtcg cggaagtgca
     3901 tgcccgcata ggaattccgg tagaaattgt cgagatgggg tttcgggtgc tgaaaaaaat
     3961 cctctatccg gtaatcttct cttcggatta ttctgccgca gaaaaacttc aggtctacca
     4021 tttctcgatt aacagtattg atatcgctat ggaagtgatg acccgcgcgt ttacctttag
     4081 tgacagtagt gcctcaaagg aagatgaaaa ctatcgtatc ttctcgttac tggaaaacgc
     4141 cgaagaagaa aaagaacggc aaatagcctc aatactttca tgggaaatag atattatcta
     4201 taaaatcctg ctggattctg atttaggcag tagtttgcct ttaagccagg ctgattttgg
     4261 cctgtggttt aaccataaag gtcgacatta ttttagcggc attgccgaag taggccatat
     4321 ctcccgtctg attcaggatt tcgacggtat tttcaatcaa accatgcgta acaccaggaa
     4381 tttgaataac agaagtttgc gggtgaagtt tctattacag ataagaaata ccgtatcgca
     4441 aattattacc ttgctgcgtg aattgtttga agaagtatcg cgccacgaag tcggtatgga
     4501 tgtactgacg aaattactta accgccgttt cctgccgact atcttcaaac gcgaaattgc
     4561 ccatgccaac cggaccggta caccgctgtc agtgctgatt attgacgttg ataaattcaa
     4621 agagatcaac gatacgtggg gccataacac tggcgatgaa attctgcgta aagtctctca
     4681 ggccttttat gacaacgtcc gcagtagtga ttatgttttc cgctacgggg gcgatgaatt
     4741 tatcattgtt ttgactgaag cttctgaaaa cgaaacgtta cgtaccgcag aacgtattcg
     4801 cagtcgggtg gagaaaacca aactgaaagc cgcaaacggc gaggatattg ccctctcact
     4861 ttccatcggt gccgccatgt ttaatggtca tcctgactat gagcgcctca ttcaaatagc
     4921 cgatgaagct ctgtatatcg ccaaaagacg aggtagaaac cgtgttgaac tctggaaagc
     4981 cagtctttag atgcgccagg atgcagaggt aatcatgaag ctaaccgatg cggataatgc
     5041 cgccgatggc atttttttcc ccgcccttga gcaaaatatg atgggtgcgg tgttaattaa
     5101 cgaaaatgat gaagtgatgt ttttcaaccc cgccgcagag aagctctggg ggtacaaacg
     5161 tgaagaagtc attggcaata acattgatat gctgattccg cgggatttgc gtcctgcgca
     5221 tcctgaatac attcgtcata accgtgaagg cggtaaagcg cgtgtcgagg ggatgagtcg
     5281 ggagctgcag ctggagaaaa aagacggcag taaaatctgg acccgttttg cgctatcgaa
     5341 agtgagcgcc gaggggaaag tttattacct ggcgctggta cgggatgcca gcgtagaaat
     5401 ggcgcaaaaa gaacagactc gacaattgat tattgccgtt gaccatctcg accgaccggt
     5461 gattgtcctc gatccggaac gccatattgt gcagtgcaat cgcgcattta ccgaaatgtt
     5521 tggttactgc attagcgaag ccagcggtat gcagcccgat acactcctga acattcctga
     5581 attccctgcc gataaccgca ttcgtttaca acagttgcta tggaaaaccg cccgcgatca
     5641 ggatgaattt ctgctgttga cgcgcaccgg tgaaaaaatc tggattaaag cctctatcag
     5701 cccggtttat gacgtgctcg cgcatctgca gaacctggta atgaccttct cggatatcac
     5761 cgaagaacgg caaatccgcc agcttgaagg caatattctc gccgccatgt gcagcagccc
     5821 gccatttcat gaaatggggg aaatcatttg tcgtaacatc gaatctgttc tcaacgaatc
     5881 gcatgtttcg ctgttcgcac tgcgcaacgg gatgccgata cactgggcgt catcttcgca
     5941 cggtgcagaa gttcaaaatg cgcaaagctg gtcagcgacc attcgtcagc gtgatggcgc
     6001 gcctgcgggg atcctgcaaa ttaaaacctc gtcaggagca gaaaccagcg cctttatcga
     6061 acgcgtggca gatatcagtc agcatatggc cgcgctggcg ctggaacagg aaaaaagccg
     6121 ccagcatatt gaacaactca tccaatttga tccgatgacc ggtctgccaa atcgcaataa
     6181 cctgcacaat tacctcgatg acctggtcga caaagccgtc tctccggtgg tgtatctcat
     6241 cggtgttgac catattcagg atgtgattga tagccttggc tatgcgtggg ccgatcaggc
     6301 attgctggaa gtggtcaatc gctttcgtga aaaactcaaa ccggatcagt atctctgtcg
     6361 tatcgaaggt acgcagtttg tcctcgtgag cctggaaaac gacgtcagta acattaccca
     6421 aatcgccgat gagctacgga atgtggtcag caagccgata atgattgacg ataaaccctt
     6481 cccgcttacc ttgagtattg gcatcagcta cgacgtgggt aaaaaccgcg attacctgct
     6541 ctccactgct cacaatgcaa tggattatat tcgcaagaat ggcggtaacg gctggcagtt
     6601 cttcagcccg gcgatgaacg aaatggtaaa agagcgtttg gttttaggcg cagcgctgaa
     6661 agaagcgatt agcaataacc aactgaaact tgtttaccag ccgcaaatct tcgcagaaac
     6721 gggtgaactg tacggcatcg aagcccttgc gcgctggcac gatccccagc atggtcatgt
     6781 gcccccttca cggtttattc ctctcgcaga agagattggt gaaatcgaaa atattgggcg
     6841 ctgggtcatc gcggaagctt gccgtcagtt agcagaatgg cgtagccaga atattcatat
     6901 cccggcgctt tccgttaact tgtcggcact gcactttcgc agtaatcaac tgcctaatca
     6961 ggtgtctgat gcaatgcacg cctggggtat tgacggccac cagctgacgg tagaaatcac
     7021 ggaaagcatg atgatggaac acgataccga aatctttaag cgcattcaga tcctgcgtga
     7081 tatgggcgtg ggcttatcgg tagatgattt tggtacgggc ttttccggat tatcccgctt
     7141 agtcagtctt ccggtaacgg aaatcaaaat tgacaaaagt tttgtcgatc gttgtctgac
     7201 cgaaaaacgc atccttgcct tacttgaagc cattaccagc attgggcaaa gcctcaattt
     7261 aaccgtcgtg gcggaagggg tcgaaaccaa agagcaattt gagatgctac gcaagatcca
     7321 ctgtcgcgtt attcagggat atttcttttc ccgcccccta cccgccgaag aaattccagg
     7381 ctggatgagc agcgtgttac cgctgaaaat ctgacaaatt cctctcgccc gcactcgcgg
     7441 gttttcattt aacgtgacac tgtcacttaa atactgtgat ttcagctacg attccgggag
     7501 attccttcct taacttcccc tcttgcacca aaactgcact acgctgcaca aatgttgatc
     7561 aaaagttaaa aaatataact ttaagccagc attctggcgc ttatcccggc atggcatgag
     7621 atctgcataa gcggaaagcg cagcaatttt tgtcttatac aggaaccgtt tatgtcggat
     7681 accaccgaac tggttgattt agccgtaatc ttccctgatc tggagatcga attgaaatac
     7741 gcctgcgctg ataacatcac aggtaaagct atttatcagc aagcgcgttg tctgctacac
     7801 aaggatgcga ttaccgcgct ggcgaaaagt atcagtatcg cccagctgtc aggcttacaa
     7861 ctggtgattt acgatgcgta tcgcccacaa caagcacagg cgatgttgtg gcaagcctgc
     7921 ccagacccgc aatatgttgt tgatgtgacg gtcggttcta atcacagccg tggcacggcg
     7981 atcgacctga cgcttcgtga tgagcacggg aacatcctcg atatgggcgc agggttcgat
     8041 gaaatgcacg agcgctccca tgcctatcac ccttccgtcc cgcccgccgc tcagcgcaat
     8101 cggctgttgc tgaatgcgat aatgactggt ggcggttttg tcggtatctc cagcgaatgg
     8161 tggcacttcg aattacccca ggcagcgagt taccctctgc tcgctgatca attcacctgt
     8221 tttatatccc ctggcacaca acacgtcagt taatcaggaa actgtcatga agagatcgat
     8281 accgtttcgt cccacattgc tcgcgctcgt ccttgccaca aatttcccgg ttgcgcacgc
     8341 cgccgtaccg aaagatatgc tggtgattgg taaggccgcc gatccacaaa ccctcgaccc
     8401 ggcggtaaca atagataata acgactggac ggtgacctac ccgtcttatc agcggctggt
     8461 tcagtacaaa acggacggtg ataaaggctc aaccgacgtt gaaagcgatc tggcaagtag
     8521 ctggaaagcg tctgacgatc aaaaagagtg gacgttcacc ctgaaggaca atgctaaatt
     8581 tgccgatggc acaccggtca ctgccgaagc agtaaaactc tcttttgagc ggctactaaa
     8641 aatcggccaa gggccagcag aagcatttcc caaagattta aagattgatg ctcccgacga
     8701 acatacggtg aagtttaccc ttagccagcc attcgcaccg ttcctctaca cgctggcgaa
     8761 tgacggtgca tccattatca atccggcggt cttaaaggaa catgcagcgg atgatgctcg
     8821 cggcttcctc gcgcaaaata ccgccggttc cggaccattt atgctgaaaa gctggcaaaa
     8881 aggtcagcaa ttagttctgg tgccaaatcc gcattacccc ggcaataaac cgaactttaa
     8941 gcgagtatcg gtaaaaatta tcggtgaaag tgcctcccgt cgcctgcagc tctcccgtgg
     9001 cgacattgac attgccgatg cgctgccggt ggatcaactc aacgccctga agcaggaaaa
     9061 taaagtcaat gtggcagagt atccgtcact gcgcgttacc tatctgtatc tgaataacag
     9121 caaagcgcct cttaatcagg cggatctgcg tcgggccatt tcctggtcta ccgattatca
     9181 gggcatggtt aacggcattc tgagtggtaa cggaaaacag atgcgcggcc cgattccgga
     9241 aggcatgtgg ggctacgatg cgacggcaat gcaatacaac catgacgaaa cgaaagccaa
     9301 agccgaatgg gataaagtga cgagcaaacc caccagcctg acgtttctct actccgataa
     9361 cgatccgaac tgggagccta ttgctttggc gacacaatcc agtctcaaca agctgggcat
     9421 caatgtgaag ctggaaaagc tggcgaacgc caccatgcgc gacagagtgg gtaaaggtga
     9481 ttacgacatt gcgattggca actggagtcc ggattttgcc gacccgtata tgtttatgaa
     9541 ttactggttt gagtcagaca aaaaaggtct gccgggtaac cgctcgttct atgaaaacag
     9601 tgaggtcgat aagttactgc gcaatgcgct tgcgaccacc gaccagacgc agcgtacccg
     9661 ggactaccag caggcacaga aaatcgtcat tgatgacgct gcttatgtgt acctgttcca
     9721 gaaaaactac caactggcga tgaacaaaga ggtgaaaggc tttgtgttca atcccatgct
     9781 ggaacaggtc ttcaatatca ataccatgag taaataacgt gaccttctgg agtattttac
     9841 gccaacgctg ctgggggctg gtgctcgtgg tggcgggcgt ctgcgtgatt acgtttatta
     9901 tctcgcatct gatccctggc gatccggcgc ggttactggc gggtgaccgc gccagcgatg
     9961 ctatcgtgga aaatattcgc cagcaactgg gactggacca gccactgtat gtacagtttt
    10021 accgctacgt cagcgacctg tttcatggtt acctgggaac atccattcgt accgggcgtc
    10081 cggtgctgga agagttgcgt atatttttcc cggcgacgct ggagctggct tttggtgccc
    10141 tgctgctggc actcctgatt ggcatcccgc tgggcatact ctctgcagtc tggcgaaatc
    10201 gctggctgga tcatctggtg cgaataatgg ccattaccgg aatctccaca cctgcgttct
    10261 ggcttgggct gggcgtcatt gtgctgtttt atggtcatct gcaaattctt cccggcggcg
    10321 gaaggcttga tgactggctg gatccaccaa cgcacgttac cggcttttat ctgctcgatg
    10381 cgctgcttga aggcaacggt gaagtcttct tcaatgcgtt gcaacatctc atcttaccgg
    10441 cattaacgct ggcgttcgtt cacctgggaa ttgtcgctcg ccagatccgc tcagcgatgc
    10501 tggaacaatt gagtgaagac tacattcgta ccgcccgggc cagcggcttg cccggctggt
    10561 atatcgtttt atgttatgcg ctacccaatg cgttgatccc atcgattacc gtattgggtt
    10621 tggcgctggg cgatttgttg tatggcgcag tgctcaccga aaccgttttt gcctggcccg
    10681 gaatgggtgc atgggtagta acatcaatac aggcgctcga cttcccggca gtgatgggct
    10741 ttgccgtcgt ggtttcattt gcttatgtgc tggtcaacct ggtggtggat ttgctctatt
    10801 tgtggattga tccgcgtatc ggacgtggag gtggtgaatg atgctaagcg aggaaacgtc
    10861 cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca aaactggtat ggatgctgaa
    10921 aggcagtccg ctcaccgtga ccggcgcagt catcattgta ttaatgctat tgatgatgat
    10981 tttttcaccg tggctggcga cgcatgatcc caacgccatt gatttaaccg cccgcctttt
    11041 gccgccttct gcggcgcact ggtttggcac cgatgaagtg ggacgcgatc tgttcagccg
    11101 cgtactggtc ggcagtcagc aatcaattct cgccggatta gtcgtggttg ccattgcggg
    11161 tatgattggt tcgctactcg gatgtctatc cggtgtgctt ggcggacgcg cagacgccat
    11221 catcatgcgc atcatggaca ttatgctgtc gattccttcg ttggtactga caatggcact
    11281 ggctgccgct ctcgggccga gtttatttaa cgccatgctg gcgattgcta ttgtgcgaat
    11341 tcccttttat gtgcgcctgg cgcgggggca agcattagtt gtacgccagt atacctatgt
    11401 tcaggcggcg aaaacctttg gtgcgtcgcg ttggcatctg atcagctggc atattttacg
    11461 taactccctg ccgccgctga tcgtgcaggc atcgctggat atcggtagcg cgattttaat
    11521 ggccgccacg ttgggattta ttggcctggg tgctcaacaa ccgagtgctg aatggggcgc
    11581 gatggtagcg aatggtcgca actatgtact cgatcagtgg tggtattgcg catttccggg
    11641 ggcagcgatt ttgcttaccg ccgttgggtt taatctcttt ggtgatggta ttcgcgatct
    11701 gcttgacccg aaagcaggag gaaagcagtc atgacccaac ccgttctgga cattcaacag
    11761 ctgcatttga gtttccccgg ttttaacggt gatgttcacg cgctcaacaa tgtgtccttg
    11821 cagattaacc gcggcgaaat tgtcggtctg gtgggagaat ccggctcagg taaatcagtc
    11881 accgcaatgc tgattatgcg tctgctacag acgggcagtt attgcgtaca tcgggggcag
    11941 atttcactgc tgggagacga tgttcttaac gctcgggaaa aacagcttcg tcagtggcgc
    12001 ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg ccctcaatcc gacacgtcga
    12061 ataggtcttc agatgatgga cgtgatccgc catcatcaac caataagtcg tcgggaagcc
    12121 agagctaaag cgattgccct gctggaagag atgcaaatcc cggatgccgt ggaagttatg
    12181 tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc gggtaatgat tgcgctggca
    12241 ttctcctgcg agccgcaatt gattattgcc gacgaaccga ctacggcgct ggacgtcacg
    12301 ctacagttgc agctactgcg tttgcttaaa cataaagccc gcgccagtgg aactgcggta
    12361 ctgttcatca gccatgatat ggtcgtggtg tcgcaactgt gcgatagcgt ttacgtgatg
    12421 tatgccggaa gcgtaattga aagcggcgtg acggcagaca ttatccatca tccccggcat
    12481 ccgtatacca ttggtttgct gcaatgcgca ccagaacatg gagtaccacg ccagccatta
    12541 cccgccattc cagggacggt accaaacctc acccatttgc ctgaaggctg cgcttttcgc
    12601 gatcgttgct atgcggcagg tgcacagtgt gaaaacgtcc cggcgctgac agcgtgtggt
    12661 gacaacaacc agcgctgcgc ctgttggtat ccgcagcagg aggtcataag tgtctgacac
    12721 gttattaacg ttacgcgacg tccatatcaa tttcccggcc cgtaaaaact ggcttggtaa
    12781 aactacggaa catgttcatg ccattaatgg tattgattta cagatccgcc gtggtgaaac
    12841 cttagggatc gtcggcgagt caggctgcgg caaaagcacc ctcgcacagc ttttaatggg
    12901 catgctgcaa ccgagccacg ggcagtacat ccgttcaggc tcacaacgca ttatgcagat
    12961 ggtgtttcag gacccgctct cttcgcttaa tccgcgctta ccggtgtggc gcatcatcac
    13021 agaaccgctc tggatagcta agcgtagtag tgaacaacag cggcgagcgt tggcagagga
    13081 gctggctgtg caggtgggta ttcgtccgga gtatctcgac cgcctgcctc atgcgttctc
    13141 cggcgggcag cggcaacgta tcgccattgc cagagcactc tcttcgcagc ctgacgtgat
    13201 tgtgcttgat gagccaacct ctgcgctgga tatctccgtg caggcgcaga tcctcaattt
    13261 actggtaacg ctacaggaaa atcacgggct gacctatgtg ctgatttcac acaatgtctc
    13321 ggtgatacgt catatgagcg atcgggtggc ggtgatgtat ctcgggcaga ttgtagaact
    13381 gggcgacgcg caacaggtgc taacggctcc ggcacatcca tacacccgat tattgctgga
    13441 ttccctcccc gccattgata aaccgctgga ggaagaatgg gcattacgta aaacggatct
    13501 gccgggaaac cgcacgttgc cgcaaggctg ttttttctac gaacgttgcc cccttgcaac
    13561 cagcggatgt gaagtccggc aatcattagc aataagggag gacggacgtg agctccggtg
    13621 ctggcgggcg ctgtagagat gctaacaacg catcaggcat tcacgcgcct gacgcgtcat
    13681 ccggcaatgc tttacgattt caactggtaa tccagcgtaa tttccgcttt cagtacctga
    13741 gaaaccgggc atcctgcttt tgctttctgg atgatgccgt caaaggtaga ggcatcaata
    13801 cccggcaccg caacttcact cttcagtgcg attttcgtaa tcgcaaaacc ggcatccact
    13861 ttatccagcg acacatcggc ggtggtatca atcgatgttg gcgtaaatcc cgcttccccc
    13921 agcattaatg aaagcgccat tgagaaacat gcggcatgcg ctgcgccaat cagttcttca
    13981 gggttagttc ctttttcgcc ttcaaaacgc gtgttaaatc catacggctg ttggttcagc
    14041 acaccactct cggtggatac tgttcccttc ccgcgtttga tatcgccttc ccagtgtgcc
    14101 tgacctttct tatggattgt cattgttgct ctcctgtggg cttaaaaagg aaattataga
    14161 ctacgtgaga aacagcacga ggataagcag gatattccga ataaaaatcc ggcagaaata
    14221 ttacttctgc acttaaatta aatttccaca atagataacc gggaatatat aacccaagag
    14281 atattgcatt aatattaatc aactcaccac aataataata cctatcaata tcatgaggtt
    14341 aaaatataaa aatcatcaac caggactaat cttaacaacg aagcggcaaa tatttgccat
    14401 gctgaatgtg ctttataaat ctgcgatccg tagcagacac cataaataca cagacacgga
    14461 gaatcactat gtttacttat tatcaggcag aaaattcaac agcagaaccc gctctggtta
    14521 atgccattga gcaaggcctt cgggcagagc acggtgttgt cactgaagat gacattctca
    14581 tggagctgac caaatgggtg gaagcttctg ataacgacat cctcagtgat atctaccagc
    14641 aaacgatcaa ttatgtggtc agtggccagc accctacgct ttaaggtgct atgcttgatc
    14701 ggcaacctaa tttaggggtt tagcacgtgt ttcttcgcta cggcgatgtt gtccttaaaa
    14761 ctagcaacag gattgaggag ttaaaatgaa atcgaaccgt caggcacgtc atattcttgg
    14821 actggaccat aaaatttcta accagcgcaa aatagttacc gaaggtgaca aatccagcgt
    14881 ggtaaataat ccaaccggca gaaaacgccc cgctgaaaag taattcataa ccatcagtcc
    14941 tcaatgacga ttaaacacca ttgcctgcgc aatggtgttt ttgtttttat ctgctttata
    15001 cttggggccg acgccctggc ggtaaagcaa agacgataaa agcgtgccag ggatggatat
    15061 tcaaaaaaga gtgagtgaca tggaaccaaa aacaaaaaaa cagcgttcgc tttatatccc
    15121 ttacgctggc cctgtactgc tggaatttcc gttgttgaat aaaggcagtg ccttcagcat
    15181 ggaagaacgc cgtaacttca atctgctggg gttactgccg gaagtggtcg aaaccatcga
    15241 agaacaagcg gaacgagcat ggatccagta tcagggattc aaaaccgaaa tcgacaaaca
    15301 catctacctg cgtaacatcc aggacaccaa cgaaaccctc ttctaccgtc tggtaaacaa
    15361 tcatcttgat gagatgatgc ctgttattta taccccaacc gtcggcgcag cctgtgagcg
    15421 tttttctgag atctaccgcc gttcacgcgg cgtgtttatc tcttaccaga accgccacaa
    15481 tatggacgat attctgcaaa acgtaccaaa ccataatatc aaagtgattg tggtgactga
    15541 cggtgaacgt attctggggc ttggtgacca gggcatcggc gggatgggca ttccgatcgg
    15601 taaactgtcg ctctataccg cctgtggcgg catcagcccg gcgtataccc ttccggtggt
    15661 gctggatgtc ggaacgaaca accaacagtt gcttaacgat ccgctgtata tgggctggcg
    15721 taatccgcgt atcactgacg acgaatacta tgaattcgtt gatgaattta tccaggctgt
    15781 gaaacaacgc tggccggacg tgctgttgca gtttgaagac ttcgcacaaa aaaatgcgat
    15841 gccgttactt aaccgctatc gcaatgaaat ttgttccttt aacgatgaca ttcagggcac
    15901 tgcggcggta acagtcggca ccctgatcgc agcaagccgc gcggcaggtg gtcagttaag
    15961 cgagaaaaaa atcgtcttcc ttggcgcagg ttcagcggga tgcggcattg ccgaaatgat
    16021 catcgcccag acccagcgcg aaggattaag cgaggaagcg gcgcggcaga aagtctttat
    16081 ggtcgatcgc tttggcttgc tgaccgacaa gatgccgaac ctgctgcctt tccagaccaa
    16141 actggtgcag aagcgcgaaa acctcagtga ctgggatacc gacagcgatg tgctgtcact
    16201 gctggatgtg gtgcgcaatg taaaaccaga tattctgatt ggcgtctcag gacagaccgg
    16261 gctgtttacg gaagagatca tccgtgagat gcataaacac tgtccgcgtc cgatcgtgat
    16321 gccgctgtcc aacccgacgt cacgcatgga agccacaccg caggacatta tcgcctggac
    16381 tgaaggtaac gcgctggtcg ccactggcag tccgtttaat ccagtggtat ggaaagataa
    16441 aatctaccct atcgcccagt gtaacaacgc ctttattttc ccaggcatcg ggctgggtgt
    16501 tattgcttcc ggcgcgtcac gtatcaccga tgagatgctg atgtcggcaa gtgaaacgct
    16561 tgctcagtat tcgccgctgg tcctgaacgg cgaaggtctg gtactaccgg aactgaaaga
    16621 tattcagaaa gtctcccgcg caattgcgtt tgcggttggc aaaatggcgc agcagcaagg
    16681 cgtggcggtg aaaacctctg ccgaagccct gcaacaggcc attgacgata atttctggca
    16741 agccgaatac cgcgactacc gccgtacctc catctaagcc tgcgcccggt agtgaaggct
    16801 accgggctat ttccctctcc ctttttcaga tctcatccat actgggtagt ggcgaataaa
    16861 tctcatttgc ctcacctgct atgcagaaca tcatccgaaa aggaggaact atgaaggctg
    16921 cagttgttac gaaggatcat catgttgacg ttacggataa aacactgcgc tcactgaaac
    16981 atggcgaagc cctgctgaaa atggagtgtt gtggtgtatg tcataccgat cttcatgtta
    17041 agaatggcga ttttggtgac aaaaccggcg taattctggg ccatgaaggc atcggtgtgg
    17101 tggcagaagt tggcccaggt gtcacctcat taaaaccagg cgatcgtgcc agcgtggcgt
    17161 ggttctacga aggatgcggt cattgcgaat actgtaacag tggtaacgaa acgctctgcc
    17221 gttcagttaa aaatgccgga tacagcgttg atggcgggat ggcggaagag tgcatcgtgg
    17281 tcgccgatta cgcggtaaaa gtgccagatg gcctggactc ggcggcggcc agcagcatta
    17341 cctgtgcggg ggtcaccacc tacaaagccg ttaaactgtc aaaaattcgt cccggtcagt
    17401 ggattgctat ctacggtctt ggcggtctgg gtaacctcgc cctgcaatac gcgaagaatg
    17461 tctttaacgc gaaagtgatc gccattgatg tcaatgatga gcagttaaaa ctggcaaccg
    17521 aaatgggtgc agatttagcg attaactcac gcaccgaaga cgccgccaaa attgtgcagg
    17581 agaaaaccgg tggcgctcac gctgcggtgg taacagcagt tgctaaagct gcgtttaact
    17641 cggcagttga tgctgtccgt gcaggcggtc gtgttgtggc tgtcggtctg ccgccagagt
    17701 ctatgagcct ggatatccca cgccttgtgc tggatggcat tgaagtggtc ggttcgctgg
    17761 tcggcacgcg ccaggatcta accgaagcct tccagtttgc cgccgaaggc aaagtggtgc
    17821 cgaaagtcgc cctgcgtccg ttagcggaca tcaacaccat ctttaccgag atggaagaag
    17881 gcaaaatccg tggccgtatg gtgattgatt tccgccgtta agaggccttt gctgcgagtg
    17941 ccagtcctgg tgtcgcagca tcgcactctc tccgacatta aatggctaaa tggattattg
    18001 ttccttccta cgcaacaata atgatagtgt tcctgatgaa ggaacactac tgagagtaaa
    18061 gacactgatg aaaatggcca atcatccccg cccgggggac attattcagg aatcactgga
    18121 cgaacttaat gtcagcctgc gcgagtttgc cagagcaatg gaaattgctc cctcaacggc
    18181 aagccgattg ctgaccggaa aagcagcttt gacaccagaa atggcgatta aactttccgt
    18241 ggtgatcggc agttcgccgc aaatgtggct gaatctgcaa aacgcctgga gtctggcaga
    18301 ggcagaaaaa acggtggatg tgtcgcgact acgccgtctg gttacgcaat aaaaaaatgg
    18361 cgcttcttaa agcgccattt tcagcttaaa gttcaaaaga gattatatcc cttcttcact
    18421 ctcttttttc gcttctgcct tctcgatttc acgataccag cgcggatggt gtttcttcgc
    18481 ccagcgacgg ctcaccttcc cttcgatcat ccctttaatc gaccctttca cccagaatgc
    18541 catatacata tggatcagga tggcgtggat caggatgata cccgcagccg cgtggatcag
    18601 caggctgtag cgaacaacct gcatcgggaa gtactgcgca aagtacggac gcaagataat
    18661 caccccggtc accagcagca cgaaaatcat gctcatgatc gaccagaaca tcatcttttg
    18721 cccggcgttg tacttaccga catccgccac tttatgctca ttgcctttca atacttcgat
    18781 aatgttcaac agccacggaa tatctttctt atccgggatg ttgtgatgca caaaacgcac
    18841 aaacataaac atcagtgcga cgaaaatcgc aatgccgaaa aacgggtgca aaatgcgtcc
    18901 catctgcggc gtaccgaagg tttgcgtcag ccattgcagc gtcgggaaga agaacgaaat
    18961 cccggacagc gccaccagga agaagcaaat caccacggtc cagtgacagg cgcgatcaat
    19021 aaatttggtg cgcacaatca ttttcgactt actcatgatg atcctcctcg tcatcgtcca
    19081 cttccttatt cgggccaata ccgatgtagt ggaaaatcaa cccggcaaaa gtggcaataa
    19141 agccagccgc tgccagcggt ttcaacgcgc ctttccacag gcttaccgag gtgtcgatct
    19201 tcggatcttt cggcagaccg tgatacagct ccggctgatc ggcgtgatgc agcacgtaca
    19261 taacgtgcgt accaccgacc ccttccgggt tgtagacgcc agcatgttcg taaccacgcg
    19321 ctttcagttt cgccacgcgc tgttccgcca gctccagcat ctccttcttg gtgccgaagt
    19381 ggatagcccc ggtcggacag gttttcacac aagccggttc ctggccgacg ctgacgcgat
    19441 cgacgcagag cgtgcattta tatacccggt tatcctcttt gttgaggcgc ggaatattaa
    19501 acggacaccc ggcaatgcag taaccacagc cgatgcagtt ttccgactgg aaatcgacaa
    19561 tcccgttagc gtactgaatg attgcaccag cagacgggca cgccttcagg cagccgggat
    19621 cttcacagtg catacagccg tctttacgga tcagccactc cagcttgccg ttctgttcgg
    19681 tttcgctaaa gcgcatcacc gtccaggact tggcgctcag atcggcgggg ttatcgtaaa
    19741 ccccgacgca gtgccccact tcatcacgga tgtcgttcca ctccgaacac gccacctgac
    19801 aggctttaca gccgatacag gtggaaacgt cgataagttt tgcgacttct gctttgtaat
    19861 cacgcacctg agaaggcggc gtgatggagt tagttgcgga ccttttgata atgtcctgcg
    19921 tttccatagc catctgttcg cccccttacg ccttctcgat gttgactaag aacgctttat
    19981 attccggcgt ttgcgagttt gcatcaccga cattcggcgt cagagtgtta gcgatataac
    20041 ctttacgcgc gacaccctca aagccccagt ggattggaat acccaccgtt tcaacctgct
    20101 gaccatttac attcagtagt ttcagacgac gcgttaccac agccaccgcg cggataaagc
    20161 cacgcttgct tgagacagtg acacgatcgc cattattaat gcctttcgcc gccgccagcg
    20221 tttcgctgat ttccacaaac tgttccggct gagcaattgc gttgagcaat gcgtgcttgg
    20281 tccaggtgtg gaagtgctcg gtcagacgat aggtcgtacc cacatacggg aactgctctt
    20341 ttttacccat ccgcagtgcg tcttgttcat acagacgaac aaccgggtta gacaccacgt
    20401 tcggatgcag cgggttagtg cccagcggcg tttcaatcgg ctcgtagtgt tccgggaacg
    20461 gaccttccgc cattttgttg atggcaaaca ggcgtcccat cccttccggc tgcatgataa
    20521 acggcccggt tggcgtaccc ggtgcggcat tgccgaagtc aggaatatcg ttacccgtcc
    20581 acttgctgcc gttccactgg atcagcatcc gtttcggatc ccacggttta ccgttgatat
    20641 ccgccgaagc acggttgtac agcacgcgac ggttgagcgg ccacgcccag gcccatccca
    20701 gcgtattccc cagaccggac gggtcggagt tatcgcggtt agccatctgg ttgccctgct
    20761 ctgtccagct accggtgtag atccagcaag aagatgcggt tgtaccgtca tcacgcagat
    20821 gcgcaaagct actcagcaac tgacctttct tcgcaatcag cacgccatta gcatcataga
    20881 gatcttccag cgcatagccg ttgttctctt tagctacttc gtcagattgc ggttcgtgcg
    20941 gctgcttgta gttccagctc atcttcatca gcggttctac gcctttacca ccttcggcct
    21001 ggtacagctc gcgcaggtga tggtagatac ccgccagaat ttcaccgtcg ttacgcgctt
    21061 cgcccggcgc gtcctgacct ttccagtgcc acagcagcca gcgaccggag ttagcaatag
    21121 aaccatcttc ttcagcaaag caggtcgaag gcagacggaa tacttcagtc tgaatagacg
    21181 ccggatcgac atcgttcgac tcgccgtggt tctgccagaa ggttgaggtt tcagtcacca
    21241 gcggatcgat aaccaccatg tacttcagct tgcttaggca gctcaccact ttgtttttgt
    21301 ccgggaagga cgcaaccggg ttaaagccct ggcagaaata accggtgact ttgccttcat
    21361 ccatcatgtt gaaatacttg atgacgtcgt aggtctggtc ccacttcggc agccagtcat
    21421 agccccagtt gttctctttc tgcgcggcat cgccatagaa agatttcatc aggctaacga
    21481 agaacttcgg atagttgctc cagtagttca cctgaccagc cagcgtcgct ttcggcgtgt
    21541 tcgcttccag atacgactgc aaatcaacct gtttttctga cggcagcgtc agataacctg
    21601 gcaggctggt agagagcaga cctaagtcag tcagaccctg aatgttggag tgaccacgca
    21661 atgcattcac gccgccaccg gccataccca tgttaccgag cagcaactgg atcatcgcca
    21721 tagtacggat gttctgcgca cccacggtgt gctgcgtcca gcccagcgcg tacaggaagg
    21781 tggttgtgcg atccggtgcg ctggtggagg ccagcacttc acacactttc aggaagtcgg
    21841 cttttggcgt accgcagatg ttttcaacga cgtctggcgt gtagcgggaa acgtgctctt
    21901 tcagcaggtt ccacacacag cgcggatgag tcagtgtttc atcgcgtttc gcatagccgt
    21961 tttcatcgaa ctgatagttc caggacgatt tgtcgtactg gcgtttttca gcgtcataac
    22021 cgctgaacag gccatcttcg aaagcaaaat catcacgcac cagcaggctg gcgttggtgt
    22081 aatgtttaac gtattcggcg ttgattttgt tgttttcgat caggtagcgc aaaacgccag
    22141 acaggaacgt aatgtccgta ccggaacgaa taggtgcgta aatatccgcc acagaagcgg
    22201 tacgcgtaaa acgaggatcg acaacgatca aggttgcatc gttgttgttt ttcgcttcca
    22261 tcgcccagcg gaaaccgacg ggatgcgctt cagcagcgtt accgcccatt accattacga
    22321 cgttagcgtt tttgatatcc acccagtggt tggtcatcgc accgcgacca aatgttggag
    22381 caagacttgc taccgttggt ccgtgtcaga cgcgcgcctg gttgtctacc gccagcatcc
    22441 cgagggagcg ggcaaatttc tgtgtcagca tcccggtttc gttgctggca ccggaggcac
    22501 acagcatacc ggtagaaagc caacggttta ccgttacgcc ctgctcgttc ttttcaataa
    22561 agttagcgtc acggtcagct ttcatcagct ttgcaatacg ggagaatgct tcttcccagc
    22621 taatgcgctg ccatttgtca gaacctggcg cacgatattc cgggtagcgc agacggtttt
    22681 cgctgttgac gtaatccagc aaaccggccc cttttgggca cagcgcacca cggcttaccg
    22741 gatgatccgg gtcaccttca atgtgataaa tcgcttctct ggcgtttttc gcgccatcac
    22801 ccaggctata catcaatagc ccgcaaccta cggaacagta tgtgcaggtg ttacggatct
    22861 ctttagcgcg taataatttg tagtttcgcg cctgagccag tgcttgcttc ggggcaaagc
    22921 ccaatgccgc taccgttgtt ccagccatac cgcccgcgca gattttaaaa aattgtctgc
    22981 gactgacgtc cattgctttc ctcttttttc aggggtatta ctgcgcgagg aaactaacag
    23041 taaaattctg aatctttttg cgcgaaatca aaaagaacac gtgtttgcca caaaataatc
    23101 cgcctgcgca aattgaccta cctcaatagc ggtagaaaaa cgcaccactg cctgacaggc
    23161 cagttaaaaa aatgctataa aattcagctt aatttttaac ggcaagagag acaaaacagc
    23221 gagcatgaca cgacaaaaag caacgctcat agggctaata gcgatcgtcc tgtggagcac
    23281 gatggtagga ttgattcgcg gtgtcagcga ggggctcggc ccggtcggcg gcgcagctgc
    23341 tatctattca ttaagcgggc tgctgttaat cttcacggtt ggatttccgc gtattcggca
    23401 gattccgaaa ggctatttac tcgccgggag tctgttattc gtcagctatg aaatctgtct
    23461 ggcgctttcc ttagggtatg cggcgacccg tcatcaggcg attgaagtgg gtatggtgaa
    23521 ctatctatgg cccagcctga caattctctt tgccattctg tttaatggtc agaaaaccaa
    23581 ctggctgatt gtaccaggat tattattagc cctggtcggc gtctgctggg tgttaggcgg
    23641 tgataatggg ttacattatg atgaaatcat caataatatt accaccagcc cattgagtta
    23701 tttcctggcg tttattggtg cgtttatctg ggcagcctat tgcacagtaa cgaataaata
    23761 cgcacgcgga tttaacggaa ttaccgtttt tgtcctgcta acgggagcaa gtctgtgggt
    23821 ttactatttt ctcacgccac aaccagaaat ggtatttagc acgcccgtca tgattaaact
    23881 catctctgcg gcatttacct taggatttgc ttatgctgca tggaatgtcg gtatactgca
    23941 tggcaatgtc accataatgg cggtaggctc gtattttacg cccgtacttt cctcagcgct
    24001 tgcagccgtg ctgctcagcg ccccgctgtc gttctcattc tggcaaggcg cgctaatggt
    24061 ctgcggcggt tccctgctct gctggctggc gacacgtcgt ggttaaaata attgccgggt
    24121 ctacggcccg gcagttaatc agataaattc cgacaatatt aaaaaataaa tttcaaatac
    24181 cagtaattca cct
//
LOCUS       DAZFXT010000070        24118 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.070, whole genome shotgun sequence.
ACCESSION   DAZFXT010000070 DAZFXT010000000
VERSION     DAZFXT010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24118
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.070"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1032
                     /locus_tag="ACHKW0_002963"
     CDS             <1..1032
                     /locus_tag="ACHKW0_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HFO7475764.1"
                     /translation="ALRLSVMADIATMRMKALHFWDKHGISAASEAFGVSCRTLYWWR
                     QLLNKEGPEGLIPHSKAPLVRRKKHWHPDVLKEIRRLRTELPNLGKEQIFVRLKPWCE
                     QRYLACPSVSTIGRMIAAAHDKMRMIPVRLGSRGKALLVKKRSAKPRRPKHYRPVKTG
                     ELIGMDAIELRMGELRRYVITMIDECSNYALALAVPSLNSDIVNHFFSRAARLFPVGI
                     SQIITDNGKEFLGNFDKTLQEAAIKHLWTYPYTPKMNAICERFNRTLREQFIEFNEIL
                     LFEDLALFNQKLAEYLVLYNSKRPHKALALMTPVEYILKENKNCNMWWTHTSACSFVG
                     EALTYHLLG"
     gene            complement(1171..1431)
                     /locus_tag="ACHKW0_002964"
     CDS             complement(1171..1431)
                     /locus_tag="ACHKW0_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001512982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HFO7475765.1"
                     /translation="MFRCPLCGASARIRTSRPENDSNTVRQKYYQCNNLECGVCFSTL
                     EAFHKFTSKHASGVHSSEGIPWHELPASHRGNNQMSLPLPQN"
     gene            complement(1474..2274)
                     /locus_tag="ACHKW0_002965"
     CDS             complement(1474..2274)
                     /locus_tag="ACHKW0_002965"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO7475766.1"
                     /translation="MTVSARSADFRDEFNVKREVSWHDVTVERVVSAIAHRYGLKPQI
                     SEMLMDIEIDHADQTEESDMSFLTRMAEMLGAITTVKSGNLLFIMPGGGVNAQGQPLP
                     SFAITRSSGDRHQFRIADREAYTGVRAYWLDLNYGKKKKVSVKRRKPPKPKKEKSSSR
                     EGDYMEGAEGNVFVLRKTYQNEQAARRAAAAKWQQLQRGAASFSITLARGRAELYPEM
                     HGTVTGFKSEIDNQDWIIAKAEHTIDNSGFTTQLELEAKIPEWIAETE"
     gene            2235..2318
                     /locus_tag="ACHKW0_002966"
                     /pseudo
     CDS             2235..2318
                     /locus_tag="ACHKW0_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2741..3925
                     /locus_tag="ACHKW0_002967"
     CDS             2741..3925
                     /locus_tag="ACHKW0_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HFO7475767.1"
                     /translation="MSETRFHGARVTENTDLVTAINDVDSSVIGIVATADDADAKLFP
                     LNKPTLLTRVNDVLGKCGTTGTLYRALKAIADQVSTKVIVVRVAEHKEEDGKTQDQLV
                     IGGSEDDGSYTGMYALLVAEQDESIGYRPRILAAPELDTEAVTKSLCVIAGKLRAFVY
                     ATCHGCNTMAEAITYRQKFNEREVMLLWPDFIAYNPKSGKNETFPAPAYACGLRAYID
                     HEQGWHKSLSNVPVKNVLGMSRHVFWSLQAEDSDANSLNNKEITTIIRRNGFRFWGNR
                     TPETNAYIFEVYTRTAQVLADSIAEAQFETIDSPLTPANVKDVISAIRAKLDSLVTAG
                     KLIGAECWYDVVDNGTTDLRQGRVRIRYKYTPVPPLEDMELYQTFTDEFFGPAFAVLG
                     GA"
     gene            3925..4437
                     /locus_tag="ACHKW0_002968"
     CDS             3925..4437
                     /locus_tag="ACHKW0_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HFO7475768.1"
                     /translation="MAVPKHLRFFTLFVDGENEVGKVTSVTPPKLTRKTDSYRGGGMM
                     GAVSIDLGLDDSALDASFVMGGAVRALFLKYGGTIDGTLLRFAGEYYTDAESDLYEIE
                     MRGRVTEIDMGEAKQGEATSHTYAVKNTYYKLSVNDRPLWEIDLLNHIYRKDGKDIVP
                     DRIRSALGLG"
     gene            4493..4867
                     /locus_tag="ACHKW0_002969"
     CDS             4493..4867
                     /locus_tag="ACHKW0_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7475769.1"
                     /translation="MKDIDTETRNNTVADDVTAGEDMAVERGVKLTRPIERGGEKITY
                     VEITGAIEQAGSLRGLSLSDVLNLKADTMFMLLPRVTSPRLDEVMIKKMSSRDFIQLC
                     AVAVNFMSEPDSGAKSVQETAA"
     gene            4876..5031
                     /locus_tag="ACHKW0_002970"
     CDS             4876..5031
                     /locus_tag="ACHKW0_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HFO7475770.1"
                     /translation="MCFEHIEDLVADIAAIFNWSPAEIFMMTPGEVVSWRERAALRSG
                     NADNEDS"
     gene            5018..7825
                     /locus_tag="ACHKW0_002971"
     CDS             5018..7825
                     /locus_tag="ACHKW0_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000853438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7475771.1"
                     /translation="MKTLDIRVAFSAVDRLTRPAENARRLMGQFGDSIQRTQGAIKNL
                     ERQARSFERARDAVSKADAGIVKARRQLNALNQLQRTGTVLSEKQQKLMQQLSTRLER
                     LNESRTREIQKMRELGGELKRHGISLTGSDNTIQQAIRRTEQYNNQLERERQALARVT
                     RARERYSRAQETAGKLKTGGALAIGAAAAGGYAAGRFLQPAIGFGKEMSRVQALTRID
                     KNSPQFKALREQALKLGSETPFTASDAASGQSFLAMAGFTPQAIQAALPGVLNMALAG
                     GVELGETADIGSNILTQFNLTADQMDRVGDTLTAAFTRTNTDLRALGETMKYTGPVAA
                     KLGISLEEAAAMAGMLASNGLRGSDAGTAMRASLSRLASPPKAAADALKELGVSVADA
                     RGKMRPMEDVLLDLYKATQKYGQVDQVSFFKDIAGEEAFVGLQTLVAAAGSGELQKLT
                     RELQGARGEVDRVAKVMADNLDGDLKNLDSAWEGLRIRISDLVDGPLRSVTQWLTRVL
                     EKITSLAQAHPVLTRQLLIAGGALLAMTATIRSLSLVIGVLYGKLATLRLGFDILTRS
                     MNVVRVLPALWGMLTGSVSLLGGAIGALFSPVGLIVAALAGAAVLIWKYWDPIRAFFA
                     GVFSGIMERLTPLRETFERFGPVFDAIGSGISQVFNWFKSLLSPMESSKETLDKCTSA
                     GEIFGNVLGGALQLVLTPAKMLLDTLAWILEKLGVLPDEAERARKKIEDAQRAAILRD
                     KVALLQGDLAKINPPKPVENGNGTGGDKPKDNKPLTDSNTGTLRRLSKIADNTGKLVD
                     ETKKRIGPGDIVFKNLPRALAVRGEWQERKIAQVSKPAPAINITPVVPAPLPPALVPV
                     VAASSRPVAEAIRSPVASVPVTSRNREPVASGFGGEIHVHLHNVVTQNPRELAKLVGE
                     MVRAEMERRARAGRGSFYDKD"
     gene            7838..8326
                     /locus_tag="ACHKW0_002972"
     CDS             7838..8326
                     /locus_tag="ACHKW0_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310666.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7475772.1"
                     /translation="MMMIYGMFVFELRTLPHQQLQQNKSWRHVKNERVNRSASWQYIG
                     AGDDRIVLSGVLYPEITGGEVSLSLLTTQAYTGRPWPLIDGVGQIYGMYVLTETNTTR
                     SEFDRYGKAKKIEFSLTLERCDEDLRERLQSSSFSDMLSGFKDKVTSSLNSAASSVKG
                     LL"
     gene            8483..9055
                     /locus_tag="ACHKW0_002973"
     CDS             8483..9055
                     /locus_tag="ACHKW0_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO7475773.1"
                     /translation="MLSYLMAIHFVLFGNSTNLKNFWKHEVIRRKRMDIWRLLREKKQ
                     RNRNFLFWWRLANEMYINGNKLHKKAAKKLNSKIINKFGCEIGLGANIGKGLTIPHHA
                     GIVVHFAVDAGENLVLRQNTTIGQIDGDIPGSRVKIGSNVDIGANCCIIGLSRKIGDN
                     VKIGAMSFINKDIPSNCTYITKKSGVVLYK"
     gene            complement(9099..9677)
                     /locus_tag="ACHKW0_002974"
     CDS             complement(9099..9677)
                     /locus_tag="ACHKW0_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7475774.1"
                     /translation="MAFRMSKQSRTLKIYNLLAGTNEFIGDGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNTDEASWHLVEDHRGYTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTSWVKDAEAEKLFRSQEAEETKNSLMQVASEHIAPLQDAVDLDIATEEE
                     ASLLAAWKTYRVLLNRVDTTVAADIEWPVAPQ"
     gene            complement(9677..10582)
                     /locus_tag="ACHKW0_002975"
     CDS             complement(9677..10582)
                     /locus_tag="ACHKW0_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO7475775.1"
                     /translation="MASKGQVTALTDNTQGATGLELYEVYNNGYPTAYGNIIHLKGMT
                     AVGEGELLIGWSGTSGAHAPAFIRSRRDTTDANWSPWAQLYTSAHPPAEFYPVGAPIP
                     WPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSGVIPDMRGWTIKGKPASGRAVLSQEQ
                     DGIKSHTHSASVSSTDLGTKTTSSFDYGTKTTSLFDYGTKTTSNAGSHTHSIPTSDGA
                     NQWGKIPLTSGRLATQYTSATTGSSGSHSHTVGIGSHQHTVGIGAHTHSVAIGSHGHT
                     ITVNAAGNAENTVKNIAFNYIVRLA"
     gene            complement(11102..11947)
                     /locus_tag="ACHKW0_002976"
                     /pseudo
     CDS             complement(11102..11947)
                     /locus_tag="ACHKW0_002976"
                     /inference="COORDINATES: protein motif:HMM:NF023985.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            complement(11950..12480)
                     /locus_tag="ACHKW0_002977"
     CDS             complement(11950..12480)
                     /locus_tag="ACHKW0_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HFO7475776.1"
                     /translation="MSNSLLPPSASNFMRCAEAVGTRITDIPVDLNTLWSPDTCPVHL
                     LPYLAWAFSVDRWDRNWPEETKRQVIRDAWLIHRHKGTISALRRAIEPLGYLIRVSEW
                     WEFGGEPGTFTVEVGTLDSGVTEEMYLEMERLIADARPVSRHMTGLNIIQEIPGDIFA
                     AAATYDGEVITIYPDD"
     gene            complement(12473..13369)
                     /locus_tag="ACHKW0_002978"
     CDS             complement(12473..13369)
                     /locus_tag="ACHKW0_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HFO7475777.1"
                     /translation="MPAVDLSQLPEPAIIAEPDFEAILADTKAMMIASYPAEQREAVS
                     AALELESEPLNVIAQTMSFREMLLRQRVNEGARACMLSHGSGTNLDNLAGNMNTKRLV
                     ITPATDTTDAVMESDTSLRLRAQRAYDGLSVAGPSGAYEYFARSASGLVRDARAISPS
                     PACVTVSILSTEGDGTATEALLNTVRAVLNAEDTRPVADRLTVQSARIVTWRLNAKLY
                     FYPGPESEPILAAAESSFRKWLSEQGLIGQDVALSAIAAALHVHGVQRVEIIEPTQNM
                     AISDIQAARCESFTISEGGRNE"
     gene            complement(13373..13723)
                     /locus_tag="ACHKW0_002979"
     CDS             complement(13373..13723)
                     /locus_tag="ACHKW0_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HFO7475778.1"
                     /translation="MTTRYTGMNPDGTGNLNDMEHLKQSVRDILTTPLASRVMRREYG
                     SLVPDLIDEPMNNTTRLQCMSAAVIALTRWEPRIALDAIDVVWKAGGRAGVTLSGTVM
                     QTMQNVELTITLRE"
     gene            complement(13720..14286)
                     /locus_tag="ACHKW0_002980"
     CDS             complement(13720..14286)
                     /locus_tag="ACHKW0_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HFO7475779.1"
                     /translation="MRLLSNIIRTGIISEVDEKSWCVRVRSGELETGWLRWNTTRAGA
                     FNVWLPPSPGEQVVIACIGGNPETAMIIGSLWSDASPAPGKSLKEIVVSAPDGAVFRY
                     DADAGALSASGMKTATLQASVSVKLDTPVVECTNLLKTAEIDVTKGGKMSGNITHSGG
                     DFTSNGITVHTHKHGGVKGGSDSTGGPQ"
     gene            complement(14298..14933)
                     /locus_tag="ACHKW0_002981"
     CDS             complement(14298..14933)
                     /locus_tag="ACHKW0_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HFO7475780.1"
                     /translation="MDKAFTRVDETFEAIRDSLNQQAINNIARKLAQDLRRAQQARIR
                     SQKAPDGTAWTPRRRRVTRIQERIRFIWNNEARTLKNWHHDTGKYGRTITGWDEDKNN
                     IRTFYRDDIDRFLEIRTRRINQDSTKRVPMFVKLRTARYLKARADASGVTVGYSGVAA
                     RIARVHQFGERDQVAPGIFTDYPVRELLGISQADERLIYNTVLGRIAEAVR"
     gene            complement(14926..15393)
                     /locus_tag="ACHKW0_002982"
     CDS             complement(14926..15393)
                     /locus_tag="ACHKW0_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7475781.1"
                     /translation="MLKTDSLREAMTRSCRWCQANPEKFTIFVESGNIETTGETPSFV
                     YRYQMVMFVMDYAGELDDLTLPLLAWLSENQPQLLLNPERNQDIKFSAVINDDDSADL
                     LFTLPLRERVRITRNSQGTPQAEHLPEPKPRLPSSEGDWSHVFQDVTWGESDG"
     gene            complement(15531..15938)
                     /locus_tag="ACHKW0_002983"
     CDS             complement(15531..15938)
                     /locus_tag="ACHKW0_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HFO7475782.1"
                     /translation="MKLWPTLGVAFLLIAAWGTSMRLSWSLGRENARNEAQASALKST
                     ADTLNIISAGVQDMQQVLAQLRAENQQRNQDGEARREQLRNDIAKDECAHALPDARFT
                     DRLRRHAERATASAVSPAYTADADHTGNASPLP"
     gene            complement(15935..16327)
                     /locus_tag="ACHKW0_002984"
     CDS             complement(15935..16327)
                     /locus_tag="ACHKW0_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HFO7475783.1"
                     /translation="MSNTFKFSSRSEKNLQGVNPDLVKVTRRALEISEVDFGITEGVR
                     SRYRQKQLVATGKSQTMNSRHLTGHAVDVVAYVGSQVSWEWPQYEKIAAAFRQASREL
                     NIPVEWGGDWKTLKDGPHFQLPHGAYPA"
     gene            complement(16324..16647)
                     /locus_tag="ACHKW0_002985"
     CDS             complement(16324..16647)
                     /locus_tag="ACHKW0_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7475784.1"
                     /translation="MSTIQTGITEQVIAWLFDHLPTVYAVGAAVSISALMSLYDGRTL
                     VQTVTGSLACGVLAMAVAGSLRFFGFPEDAVTFIGASIGFMGAEKARDKVIAAFNRRV
                     KEKDE"
     gene            complement(16650..16850)
                     /locus_tag="ACHKW0_002986"
     CDS             complement(16650..16850)
                     /locus_tag="ACHKW0_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HFO7475785.1"
                     /translation="MKVKALEGDTVDSLCFRYYGTTQGVTEKVLDANPGLCQQVFLDA
                     GQEVEMPEPEKKKREMIQLWGE"
     gene            complement(16850..17344)
                     /locus_tag="ACHKW0_002987"
     CDS             complement(16850..17344)
                     /locus_tag="ACHKW0_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HFO7475786.1"
                     /translation="MSMVARTNPGPAEDDITDTDDGDTRISAGAFWPDIVLRELRLAI
                     RLPGRVTTSRLLHTATGAVAHVTRELEAWQQEQQAAGHQTLADVPAPVINGESVNLWH
                     WRNAVYTATRALILERYRDADTTDKGDRRADALDIQTSDLWRDVSWAISDILRRPRIF
                     AELC"
     gene            complement(17446..18246)
                     /gene="gpM"
                     /locus_tag="ACHKW0_002988"
     CDS             complement(17446..18246)
                     /gene="gpM"
                     /locus_tag="ACHKW0_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000632360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit"
                     /protein_id="HFO7475787.1"
                     /translation="MITPAQQHWQNVMAQRAGRANEGVDHAARTAHEEVLYRLRLAQA
                     RLKGVQARSAKAAIKKELLPDFSGWIEGTLEADGGQQDEVIATLMVWAIDCGDLPLAL
                     RIGAYVVRHNLIMPDNFGRTAATVLTEEICNPVLTQAGTDADADLSTFIEPLDTLWEI
                     VANQDMPDEVRAKLCKACAFARRGLSDADSMASSLKLLREAMHLNPNAGVKREIATLS
                     RALKKADSAAAPEDASTPQTQDESSKSKKTTRKPATRKTTATQKAKRG"
     gene            complement(18292..19344)
                     /locus_tag="ACHKW0_002989"
     CDS             complement(18292..19344)
                     /locus_tag="ACHKW0_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019028 - viral capsid [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HFO7475788.1"
                     /translation="MNLVMSDITRNKLGCYMAQQASLNNIPVSALVSRFTVEPSVQQR
                     FENASKESTEFTKRINVIGVTDQKGEKILLDTTGPIARTNTSYDGTKRRNPNNVVDLK
                     NRKYQCEQVNYDTFISYPQLDAWAAHPDFQSRISAQIARQVALDRIMIGFNGTSHADE
                     SNFSTNKLLQDVNVGWLEHIRTDASERVMNDVTLTSRNMDNTVAHAGKYANADALVQD
                     ARSSLLDEWHKEADDLVVIMGRNLFNSLRLPVLNSISGQNPNAELLAGQLILSSRTIG
                     GLGVFLAPFFPDATMLITSFNNLSIYWQKGSMRRLMKDEPEYNRIATYQSINDAYVVE
                     DYGKCAMVTGLKFADS"
     gene            complement(19368..20204)
                     /locus_tag="ACHKW0_002990"
     CDS             complement(19368..20204)
                     /locus_tag="ACHKW0_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001262634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019069 - viral capsid assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HFO7475789.1"
                     /translation="MANEKKTSRKKFRVAVSGSTVDGREISPVHLREAAENFNPDVYA
                     ARVNVEHYLSPCPSSEFSAMGDVTALSTEDITEGPLAGRTALYAEIEPTERMKQLVAD
                     GKKIYSSIELHPQFSVNGRAYLVGLAMTDTPASLGTERLKFTAQQRQAVMTFNSIQGE
                     APLISEAIESEIIEMAEQRQEEGTQWFNRVMGIIGRGRKADDASFSRIQEAVEGVATS
                     QADIIDRFNVLETRHQQDRQKITSLTTELAALKEKLRTQDGDPQNRFTATGAASDQLA
                     DF"
     gene            20359..22110
                     /locus_tag="ACHKW0_002991"
     CDS             20359..22110
                     /locus_tag="ACHKW0_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HFO7475790.1"
                     /translation="MIQDAFVRQRARQLYWQGYPPAEISRLMGINPNTIYAWKKRDQW
                     DETPPVQRVTQSIDARLIQLTEKQNKTGGDFKEIDLLTRQLKKLHDGQPDVMAAGKKG
                     RAKKLKNHFTPKQIAALREKIISRLEWHQRGWFDSLTLCREAGIRNRMILKSRQIGAT
                     WYFAQEALLMALRDDVAQPYQRNQIFLSASRRQAFQFKSIIQKAAAEVDVELKGGDKI
                     ILSNGAELHFLGTSAASAQSYTGNFYFDEFFWVSRFAELRKVAGAMATLSGLRRTYFS
                     TPSTETHEAYAYWNGDRWNEKKATHKRQRFSVDWKTLHNGLICPDRTWRQIVTLEDVV
                     NHGWKHTDIDEIRDENTEDEFLNLYMCEFVREGESAFNLNILIGCGVDGYDDWKDWKP
                     FAPRPMGNRPVWIGYDANGSSGNGDSGAVSVVVPPAVPGGRFRTVETRRVQGLEFEEQ
                     ARVIEEFTRRYNVEHIGIDVTGGNGEAVYQIVKRFFPAAIPYTFTLSSKRSLVLKMLQ
                     IMRAGRWEYDRAERELVAAFNAVRKVKTPGGFITYETDRARGISHGDLAWATMLAVIN
                     EPIGGEGENERFTVMEF"
     gene            22110..23156
                     /locus_tag="ACHKW0_002992"
     CDS             22110..23156
                     /locus_tag="ACHKW0_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7475791.1"
                     /translation="MSRKNKKVRMSSRIDLADALRKESSLSAFTFDGPYRLTGHDLLD
                     NMYCADNGRWYETPVDWYGLARAARQTSWHQSALYFKRNVLLGCYIPHPLLSRQDFSA
                     LALDWFVFGNAFLELRSNMLGEPLKLRHALAKYMRRGSDLESWWYVQDGKDAFQFRPG
                     KVCHLMNPDINQEIYGMPEYLGALLSASLSHSADMFRKLYYDNGSHAGCIIYIGAAQV
                     NRESMDSLKETLQGARGGGAFKNVLIHAPNGGKEGVQILPFQQITAKDEFMNVKAASR
                     DDVLAAHRVPPQLMGAMPGEKSAFGDVEKAARVYAINELMPVMEAMKHINDWLGEEVI
                     RFNPYALLDTQPTS"
     gene            23687..>24118
                     /locus_tag="ACHKW0_002993"
     CDS             23687..>24118
                     /locus_tag="ACHKW0_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001412162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475792.1"
                     /translation="MKKDSYPYLICMTVSVLGIIFLFYWWRADIYRITYLNSRISNYY
                     ILCSMGMSFFFSLFLVKRGVVKQSGWKGLSEYLKVYAGSCILTGIFLIIPLMMVTYFL
                     PGVTSSYVAPYEYTSGSSRSCSGAFVDDPELHKNILICYPYG"
BASE COUNT         5279 a         6269 c         6655 g         5915 t
ORIGIN      
        1 gcgttgaggc tttcagtgat ggctgatata gcaacaatga gaatgaaggc tctgcacttc
       61 tgggataaac acggtatttc tgcagcttct gaagcctttg gcgtgtcctg ccgcacgctt
      121 tactggtggc gtcagttact gaacaaggaa ggacctgagg ggctaatccc gcacagtaag
      181 gcacctctgg tgcgacgaaa aaaacactgg catcccgatg tgctgaaaga gattcgacgc
      241 ctcaggacag agctgccgaa cctcggtaaa gagcagattt ttgttcgcct gaagccctgg
      301 tgcgaacagc gctatctggc ctgtccgagt gtttccacca tcggcagaat gatcgctgcc
      361 gcacatgata aaatgcggat gatacctgtg cgtctgggat cgcgggggaa ggcactgctt
      421 gtcaaaaagc ggtctgccaa accccgcagg ccaaagcact accgcccggt aaagacaggt
      481 gagctcattg ggatggacgc cattgagctc agaatgggcg aactgcgtcg ctatgtcatc
      541 accatgatcg acgaatgcag caattacgcg ctggcgctgg ctgtgccgtc actcaacagt
      601 gatatcgtca atcacttctt cagccgcgca gcccggttgt tcccggtcgg tatcagccag
      661 ataatcacag ataacggaaa agagttcctg ggaaacttcg acaaaacgct gcaggaagcc
      721 gctatcaaac acctctggac ctatccctac acgccaaaaa tgaacgctat ctgtgaacgt
      781 tttaaccgga cgttaagaga gcagtttatt gaatttaatg agattttact ctttgaagat
      841 ctggcgctat ttaaccagaa gctggcggaa tatctggtgc tgtataacag caaaagaccc
      901 cacaaggcgc tcgcactaat gacgcccgtg gaatatattt taaaagagaa taaaaattgc
      961 aatatgtggt ggacccatac aagcgcttgc tcctttgttg gagaggcgct aacctatcac
     1021 ttgctaggtt gacgggttag ggcctcggtt aaacagaaat gttttccggg gcctttccac
     1081 atccggcctt cgggtattcc ctccgaccat cagccgaaag gcacccgcac gtaatctatc
     1141 gcttttttgt tactccggca attctgcctg ttaattctga ggtaaaggca aactcatctg
     1201 attgtttccc ctgtgtgaag ctggcagctc atgccacggg ataccttctg atgagtgaac
     1261 gccggaggcg tgtttcgatg tgaatttatg gaaagcttcc agtgttgaga agcatacgcc
     1321 gcattccagg ttgttacact ggtaatactt ttgccgcacg gtgtttgaat cattttccgg
     1381 acgactggtg cggatacggg cagatgcgcc acaaagcgga caacggaaca tagcgacctc
     1441 ctttaacgtg gtgctgccgc tattctaagt tgctcactct gtttccgcta tccattccgg
     1501 gatttttgcc tcaagctcaa gctgcgtggt aaagccgctg ttatcaatgg tgtgctcggc
     1561 ttttgcaata atccagtcct gattatcaat ctcgctttta aatcctgtta ccgtgccatg
     1621 catttcgggg tagagttctg ctcgtccacg tgccagcgtg atggagaatg atgcggctcc
     1681 gcgttgtagt tgttgccact ttgccgccgc tgcgcgtctt gctgcctgct cgttctgata
     1741 agtcttgcgt aacacaaaca cattgccttc cgcgccttcc atataatcac cttcacggct
     1801 gctgcttttc tcttttttcg gttttggcgg tttgcggcgt ttcacgctga cttttttctt
     1861 tttcccgtaa ttaagatcaa gccagtaagc gcgtaccccc gtatacgcct cgcggtcagc
     1921 aatgcggaac tgatggcgat cgccgctgct gcgtgtgatg gcgaacgatg gcaacggctg
     1981 gccctgtgcg ttcacgccac cgccgggcat gatgaataac agattaccgc tttttaccgt
     2041 ggtgattgcg cccagcattt ccgccatgcg cgtaaggaag gacatgtcac tttcttcggt
     2101 ctggtcggcg tggtcgattt cgatatccat cagcatttcg ctgatttgcg gtttcagacc
     2161 gtaccgatga gcgatggcgg ataccacacg ctcaacggtc acatcatgcc aggacacctc
     2221 acgtttaacg ttaaattcat cccgaaaatc tgcgcttctg gctgaaacag tcagcctgtc
     2281 cggcggtcct tcgtgtgcga tttcatcaac aatgtaagag cctttttctg tcagcggttc
     2341 gcctttccag ccaatgagaa ccgtcaggcg cgcgccccgt ggcggtagcc gcaactgacc
     2401 atccgcatca tccagcgtga tggtgagctg gtctgcctca aatccccggt tgtcggtcag
     2461 cgacaggctc atcaggcgct ctgccacgcc ggacagcgtt ttaccctccg cgagaatatc
     2521 aaaatccggc atttttacgg ggtctgtgcc ctgactgagc aattgcatgg tggtgtcggt
     2581 catctgttcc ctccctgtgc ggcatggtcg catgtgcgtg cggagggggt tactgctttt
     2641 tgttgtcgcc gggtcgggag aacggcgcag gggtgagatt acgcgcgtgg tgggtgatga
     2701 ttgttgccga atcatttaac ggatacaagg ggctgaagct atgagtgaaa ctcgttttca
     2761 tggtgcccgt gttacggaaa ataccgacct ggtaacagcg attaacgatg ttgattccag
     2821 cgttatcggt atcgtggcaa cggcggatga tgcggacgcg aagctgttcc cgctgaacaa
     2881 gcctacactg ctgacccgcg tcaatgacgt gctgggaaaa tgcggaacaa cggggacgct
     2941 ttatcgtgcg cttaaggcca tcgcagacca ggtgagcaca aaggtgatcg tcgttcgcgt
     3001 ggctgaacac aaagaagaag acggtaagac gcaggatcaa ctggttatcg gtggttctga
     3061 ggatgacggc agctatacgg ggatgtatgc gctgcttgtt gcagagcagg atgaaagcat
     3121 cggataccgt ccgcgtattc tggccgcgcc ggagctggac acggaggcgg tgacaaaatc
     3181 cctgtgcgtg attgcgggta aactgcgcgc gtttgtgtat gccacatgtc atggttgtaa
     3241 cacgatggct gaggcgatta cctaccgcca gaaattcaac gaacgtgagg tgatgctctt
     3301 atggcctgac ttcatcgcct acaacccgaa aagtggcaaa aacgaaacgt tccccgcgcc
     3361 tgcttatgcg tgcggccttc gtgcgtacat tgaccatgag cagggctggc acaaatcgct
     3421 gtccaatgtt ccggttaaaa atgtgctggg aatgtccagg catgtgttct ggtcgttgca
     3481 ggccgaagac agtgatgcca acagcctcaa taacaaagaa atcacgacca ttattcgtcg
     3541 caacgggttc cgcttctggg gcaaccgcac accggaaacg aacgcctaca tctttgaggt
     3601 gtatacccga accgcacagg tgctggctga ttcaattgcg gaagcgcagt ttgaaaccat
     3661 cgacagtcca ctgacgcctg cgaacgtgaa ggatgttatc agtgccatca gggcaaaact
     3721 ggattcgctg gttactgccg ggaaactgat tggtgcggag tgctggtatg acgtggtgga
     3781 taacggcacc acggatttac gtcagggacg tgtgcgtatt cgctacaaat atacgcccgt
     3841 tccgccactg gaagacatgg agctttacca gacgtttacc gatgaattct ttggtcccgc
     3901 atttgcggtg ctgggaggtg cctgatggct gtgccaaaac atcttcgctt ttttacgttg
     3961 tttgtggatg gtgaaaacga agtgggcaaa gtgacatccg tcacgccgcc caaactgacg
     4021 cgcaaaaccg acagctatcg tggtggtggc atgatggggg cggtaagtat tgatctcggt
     4081 ctggacgact ccgcgcttga tgcgagtttt gttatggggg gagctgttcg tgcgctgttc
     4141 cttaaatatg gcggcacgat tgacggcacg ctgctgcgtt ttgcgggtga atactacacc
     4201 gatgcagaaa gcgatctgta tgagatcgag atgcgcgggc gtgtgacgga aattgatatg
     4261 ggggaagcca aacaggggga agccacatca cacacttacg ctgtcaaaaa cacctactac
     4321 aagctgagcg ttaacgatcg cccgctgtgg gaaatcgacc tgttgaacca catctaccgg
     4381 aaggacggca aggacattgt gcctgaccgt atccgttccg cgcttgggct tggctgataa
     4441 gtaatatgca ggcggcgcag tgcgtcgcct ctgactgaaa aggagacaac tgatgaaaga
     4501 catcgatact gaaacccgga ataacaccgt ggcggatgat gtgacggcag gtgaggatat
     4561 ggctgtcgaa cgtggcgtaa aactgacccg accaattgag cgtggtggcg aaaaaatcac
     4621 gtatgtggag atcaccgggg ctattgagca ggctggatcc ctgcgtggtc tgtcgctgtc
     4681 tgatgtgctg aatctgaaag cggataccat gtttatgctg ttgcctcgcg tgacctcgcc
     4741 acgactggat gaagtgatga ttaaaaaaat gtcgtcacgc gattttattc agttgtgcgc
     4801 tgtggctgta aattttatga gcgagccaga ctctggcgcg aagagcgtgc aggagacggc
     4861 agcgtaatca ccctggtgtg ctttgagcac atcgaagatc tggtggcgga tattgccgcc
     4921 atttttaact ggtcgcccgc cgaaatcttc atgatgacgc ccggcgaagt ggttagctgg
     4981 cgcgagcggg cggcacttcg cagcgggaat gcagataatg aagactcttg atatccgggt
     5041 cgctttcagc gccgttgaca ggctgacccg gcctgccgaa aacgcccgcc gcctgatggg
     5101 gcagtttggt gactccatcc agcgaacgca gggggcgatt aaaaatctcg agcgtcaggc
     5161 gcgttcattt gagcgcgccc gtgacgctgt cagtaaagcg gatgctggca tcgtgaaagc
     5221 acgacgccag cttaacgccc ttaatcagtt acaacgtacg ggtacagtgc tcagcgaaaa
     5281 acaacaaaag ctgatgcagc agttaagcac ccggcttgaa cgcctgaatg aatcgcgcac
     5341 acgggaaatt caaaaaatgc gggagcttgg cggagagctg aaacgccacg gcatttccct
     5401 gacaggcagc gataacacca tccagcaggc catcagacgc accgaacagt acaacaacca
     5461 gcttgaacgc gaacggcagg cgcttgcgcg tgtaacgcgg gcgcgtgagc ggtattcgcg
     5521 cgcgcaggaa acagcgggaa aactgaaaac aggtggtgca ctggcaattg gtgcggcagc
     5581 ggcgggcggc tatgctgccg ggcgtttttt gcagcctgcg atcgggttcg gcaaagagat
     5641 gtcccgcgtt caggcactga cgcgaatcga caaaaacagc ccgcagttta aggcgctgcg
     5701 tgagcaggcg ttaaaacttg gatctgaaac accgtttact gcgagtgatg ccgccagtgg
     5761 gcagagcttt ctggcaatgg ctggttttac tccgcaggcc attcaggccg cattgcccgg
     5821 tgttcttaat atggcgctgg caggtggcgt cgaactcggc gagacggctg atataggctc
     5881 caatatcctc acacagttca acctgacagc cgatcaaatg gaccgggttg gcgatacgct
     5941 gacagcggca ttcacccgga ccaatactga tttacgcgcg ctgggcgaaa ccatgaagta
     6001 taccggtccg gttgccgcaa aacttggtat cagtcttgaa gaagcggcag ccatggccgg
     6061 gatgcttgcc agtaatggtc ttcgtggaag cgatgctggt acggccatgc gcgcaagtct
     6121 gtcccgcctt gcatcaccgc caaaagctgc ggctgatgcg ctgaaagagc tgggggtgtc
     6181 agttgctgac gccaggggca aaatgcgccc gatggaggat gtgctgcttg atctctataa
     6241 ggcgacacaa aaatacggac aggtggacca ggtttccttc ttcaaggaca tcgccggaga
     6301 agaggcgttc gttggtttgc agacgcttgt tgcggcggct ggttcaggag agctgcaaaa
     6361 actgaccaga gaattgcagg gggcaagggg agaggtcgat cgcgtcgcaa aagtaatggc
     6421 cgataatctt gatggggacc tgaaaaatct cgacagcgca tgggaaggtc ttcgtattcg
     6481 catcagtgat ctggttgacg gtccgctgcg ttctgtcacg cagtggctca cgcgggtgct
     6541 tgaaaaaatc acctcgctgg cgcaggccca tccggtactg acacgccagc tactgatagc
     6601 aggcggtgcg ttgctggcaa tgactgcaac gattcgctcg ttgtcgctgg ttattggggt
     6661 gctttacgga aagctggcca ccctgcgtct tggttttgac attcttaccc ggtcaatgaa
     6721 tgtcgtcagg gtgttgcctg cgctgtgggg aatgctgacg ggttccgttt ctttgctggg
     6781 aggcgctatc ggggcgctgt tcagtccggt tggtcttatc gtggctgcgc ttgccggagc
     6841 tgccgttctt atctggaaat actgggatcc catcagggca ttttttgccg gggtgttcag
     6901 cgggattatg gaaaggctga ccccgttgcg cgaaaccttt gaacggtttg gccctgtttt
     6961 tgacgcaatc ggaagcggga tcagccaggt gtttaactgg tttaaatcgc tgctgtcacc
     7021 gatggagtcc agcaaggaaa cgctggataa atgtaccagt gctggcgaga tattcggtaa
     7081 cgttcttggc ggtgcgttac aacttgttct gacacctgca aaaatgctac tggatacgct
     7141 ggcgtggata cttgaaaaac ttggcgtcct tccggatgaa gctgaaaggg cgcgcaagaa
     7201 aatcgaagac gcacagcgtg cggccattct tcgggacaag gttgccttac ttcaggggga
     7261 cctggcgaaa atcaatccgc cgaagcctgt ggaaaatggc aatggcaccg gaggtgataa
     7321 acccaaagac aacaaaccgc tcacagacag caataccggg acgctacgca gactcagcaa
     7381 aattgctgat aacacaggta agctggttga tgagacgaaa aaacgcattg gccccggcga
     7441 tattgtcttt aagaacctgc cccgcgcact tgctgttcgt ggggagtggc aggagcggaa
     7501 gattgcgcag gtcagtaagc ctgcccccgc aattaatatc acccccgtgg tcccggcacc
     7561 gctgcctccg gcgctggtcc ctgttgttgc ggccagctcc cgcccggtgg cggaggccat
     7621 acgatctcca gtggcatcag ttcctgtaac ttcccgtaac cgggagcctg ttgcctccgg
     7681 atttggtggt gaaattcatg ttcatctgca taacgttgtt acgcagaatc cccgcgaact
     7741 ggcgaaactg gtcggtgaaa tggtcagggc agaaatggaa cggcgtgccc gtgccgggcg
     7801 tggcagtttt tacgataaag attgaggagt catggccatg atgatgatct acggcatgtt
     7861 tgtttttgag ctgcgcacgc tgccgcatca gcagttacag caaaacaaaa gctggcggca
     7921 tgtgaaaaat gaacgcgtta accgttcagc aagctggcag tatatcggtg caggtgatga
     7981 tcgcatcgtt ctttctggtg tgctttatcc tgaaattaca ggtggcgaag tgtcgctgtc
     8041 gctgctgacc acgcaggcgt atacaggacg accctggcct ttgattgatg gcgtcgggca
     8101 gatttacggc atgtatgtcc tgactgaaac gaatacgacc cgctccgagt ttgatcgcta
     8161 cggtaaggcg aaaaagatag aattttcact gactcttgaa cgctgtgatg aggatttgcg
     8221 ggagcgcctg caatcctcat cgttcagcga tatgttgtcc ggcttcaaag ataaggtgac
     8281 atcatccctt aatagcgcgg ccagctcagt taaggggctg ctctgattta acgtatgtcg
     8341 ccaatttcct gatgaaggtg actggcgact tgctgttgta tgtccttctc agaaaattgt
     8401 ttttgaataa caaataacag gattttataa tctcttaacc ttataacatg tgtggtctga
     8461 aataataatt aaggagattt tcgtgctgtc ttacttaatg gcaattcact ttgttttatt
     8521 tgggaactct actaatttaa aaaacttctg gaaacatgaa gtaattcggc ggaaacgtat
     8581 ggatatctgg aggcttttaa gagagaaaaa gcagcgtaac cggaatttcc ttttctggtg
     8641 gcggttggct aacgaaatgt atattaatgg taataaatta cataaaaaag cagccaaaaa
     8701 gttaaacagt aaaataatta acaaatttgg ttgtgaaata ggattgggag caaatattgg
     8761 aaaagggtta acaattcccc atcatgctgg aattgttgtt cattttgctg ttgatgcagg
     8821 tgaaaatctg gtattacgac agaatactac gattggacag atagatggtg acatacctgg
     8881 ttcaagagta aaaattggta gtaacgttga tatcggagct aattgttgca tcattggatt
     8941 atcacgtaaa atcggggata atgtaaaaat aggcgcaatg tcttttataa ataaagatat
     9001 accatcgaac tgcacatata taactaaaaa gagcggtgtt gtattgtata aatagagcac
     9061 ataaagccat cgatatttct atcgatggct ttttcttttt attgtggggc gactggccac
     9121 tcaatatccg ctgctactgt tgtatcaaca cgattcaaca atacccgata tgtcttccat
     9181 gcagccagta acgatgcctc ttcttccgtc gcaatatcca aatctacagc atcctgaagt
     9241 ggcgcaatat gctcgctagc tacctgcatc aggctgtttt ttgtttcttc cgcctcctgg
     9301 ctccgaaaca gtttttctgc ttctgcatct ttcacccagg atgtaccgtt ccacttctga
     9361 aactcccctt ccggggataa ccaggtgaca ttttccggta atgggccaag ttcagaaata
     9421 aataacgcgt cgccggaagc cacgtcatag acggtataac cccgatggtc ttcaacgaga
     9481 tgccacgatg cctcatcagt gttgaaaaca gccacgaagc cagccggaat atctggcggt
     9541 gcaatatcgg tactgtttgc aggtagacct gtatgaggtg gaatatatgc gtcaccgtca
     9601 ccaataaatt cattagttcc ggccagcaga ttataaattt tgagggttcg tgattgttta
     9661 ctcattctga atgccattat gcaagcctca caatatagtt aaatgcgatg tttttgacgg
     9721 tgttttccgc gttaccagca gcgtttacgg tgatggtgtg tccatgtgag ccaatcgcaa
     9781 cggagtgcgt gtgtgcacca ataccgacag tgtgctgatg agaaccaata cctacagtat
     9841 gagaatgtga gccagaggaa cctgttgttg ctgatgtata ttgagttgcc agacgcccag
     9901 atgtcagtgg aattttcccc cactggtttg caccatctga tgttggtatt gaatgcgtgt
     9961 gactcccggc gttactggta gttttggtcc catagtcaaa tagtgaggtt gttttcgtcc
    10021 cgtaatcaaa cgacgatgtg gttttcgtac ccaaatccgt actggatacg ctggcgctgt
    10081 gggtgtgcga tttaatgccg tcctgttcct gagacaatac ggcacgacca ctggcgggct
    10141 tgcccttaat cgtccagcca cgcatatcag ggatcacgcc tgacggataa gcggctgcaa
    10201 gtttcgggta ggcagatttg tcaaaagtct gcccctgcat cagggcataa ccagacggaa
    10261 cggtatctga tggccacggg attggtgcac cgactggata aaactctgca ggaggatgag
    10321 ccgaggtgta aagctgcgcc cacggcgacc agtttgcgtc ggtcgtatcc cgtcgtgaac
    10381 gaataaatgc cggagcatga gcaccgcttg taccactcca gccgatgagt aactcacctt
    10441 cgccaacggc tgtcatccct ttcaggtgaa tgatatttcc atacgctgtt ggatatccgt
    10501 tgttatacac ctcgtataac tcaagacctg ttgccccctg cgtattgtct gtcagcgcgg
    10561 ttacctgacc ttttgaagcc agattaactg atgatactgc tgttccacct gacggtaacg
    10621 ccccgatctc tgatgccgtt ggcttatttc tggagttata gtcccttcgc cagccaggtg
    10681 aataatctgt tccgtgatta atataggtaa actgggcgtt agttgttcca ccaccagtgg
    10741 aggtggtcgg tgtggtaatg cggatcgtca tcgctgactt tatccccatt acttcaatga
    10801 cagctccggc gagatgaata ttaccgcagt cagtatcagt aatgatttta ttattgccat
    10861 aagaccagga acccttgcac atccagtatg gatggttaaa tgctccctga gaatccagcc
    10921 actcgataaa ctgtgcagtc gtccagtttc ctgttgttgt gcttactgac ccaccgaagg
    10981 cacggcaggc accaatattt ttcgtaaagg tgtctttgcc agggatatcc gcaccgttct
    11041 gatctttctg cagacgtttc tcagcattgt cataggctgt ttttactgcc tttggtgtcg
    11101 cggcaagcgt ttcagatgtg ctgttggtcg cgctgcttag ctggattatc cctttctgtg
    11161 ctgttgttgc atcctgtgcg gtgtatttcc cgttagccag gtcataggct gcctttaccg
    11221 cctttggtgt tgccgccagc gtttctgaat cgctgttggt ggcgctactg agctggacaa
    11281 tgccttttcg cgctgtagtg gcgtcctgtg cggtatattt cccgttagca aggtcatagg
    11341 ctgccttgac cgctttcggc gttgcggcca gtgcttcaga cgtgctgttg gtggcgctac
    11401 tgagttgaac aaagcctttt gcggtcagcg aggcgtccgg gtgacgtcgt gactgttcat
    11461 gttctttcag tttgtcattc acgtaatcca ctgtggccat aaccatggtg ttatccacgg
    11521 taagcgccac ggtggcagtg ctggatacgg tcagaatggt gcgaaatgtt tgtgcacgtc
    11581 cggatccttc ggcaacggct ggcttgtaac tttcggcagt attgcccacc gcgatcaaat
    11641 cgccgtgctc atcaaataca ccaatttccc ggatccagaa tccgcccgtt tcaggaggaa
    11701 taaccagctc cgcaataatg cggttctgat gtgttgcgtc caggatgacg cgattaacag
    11761 tatgtcgcca cacctcatgc accagacggg tctgcttact gtctggtgtg ggcaacgtgc
    11821 cgccaccgtc gcccacggcc atgtgagtca ggtggacagg attaccatct ggcgcggctg
    11881 cctgagctaa ttttttggca ccagtatcag tgataatggt tttaaatttt cgtgttgtgg
    11941 tactcatgct taatcgtccg gataaatggt aatgacttca ccgtcgtaag ttgctgccgc
    12001 cgcgaaaata tcccccggga tctcctgaat gatattcagc cctgtcatgt ggcggctgac
    12061 cgggcgggca tcagcaatca accgctccat ttccagatac atttcctccg tcacgccact
    12121 gtccagcgtg ccgacttcaa cggtaaatgt tcccggttct ccgccgaact cccaccactc
    12181 agacacacga atgaggtatc ccagcggctc aatggcccgg cgcagtgcgc tgatggtccc
    12241 tttgtgtcgg tgtatcagcc atgcatcacg aataacctgt cgctttgtct cttccggcca
    12301 gttgcgatcc cagcggtcaa cggaaaatgc ccaggcgaga taaggcagca gatgcaccgg
    12361 gcaggtgtcc ggcgaccaca gcgtgttgag gtctaccgga atgtctgtaa tgcgcgttcc
    12421 gacagcttcg gcacaacgca tgaaattgct ggctgatggc ggtaacagtg aattactcat
    12481 tacgcccacc ttcgctgatg gtgaatgact cacagcgcgc cgcctgtatg tcgctgatgg
    12541 ccatattctg tgtgggttcg attatctcca cgcgttgcac accgtgcaca tgcagtgcgg
    12601 cagcaatggc tgacaacgcc acgtcctgac cgataagccc ctgctcagac agccacttcc
    12661 tgaacgacga ttccgccgcg gccagaatag gttcggattc cgggccgggg taaaagtaca
    12721 gttttgcatt cagccgccat gtcacgattc tggcgctctg tacagtaagg cggtcggcca
    12781 ccgggcgggt atcctctgca ttcagaacag cgcgaacggt attaagcaac gcctccgttg
    12841 ctgtgccgtc gccctctgtg gacaggatgg aaaccgtcac acaggccgga gacgggctga
    12901 tggcccgcgc atcacgcacc agaccgctgg cgctgcgtgc aaaatactcg tatgcgcctg
    12961 acgggcctgc aacactcagg ccgtcatacg cccgctgcgc ccgcagtctc agcgaggtgt
    13021 cgctctccat caccgcgtcg gtggtatccg ttgccggagt gataaccagg cgctttgtgt
    13081 tcatattgcc cgcgaggttg tccaggtttg tccctgaacc gtggcttaac atgcaggcgc
    13141 gtgcgccctc gttaacccgc tggcgtaaca gcatttcacg aaacgacatg gtttgagcga
    13201 taacgttaag gggttccgat tccagctcca gcgcggcgga aacggcttca cgctgttcgg
    13261 cgggatagga cgcaatcatc atggcctttg tgtcagccag aattgcctca aagtcaggct
    13321 ccgcgatgat ggcgggttcc ggtaactggg aaaggtcaac ggcaggcatg atttactctc
    13381 tcagcgtgat ggttaattca acattctgca tggtctgcat gacagtgccc gacagcgtca
    13441 ccccggcgcg gcctcctgcc ttccagacaa cgtcgatggc gtccagggca atgcggggtt
    13501 cccatcgtgt cagtgcaatc acggcagcgc tcatgcattg cagacgcgtg gtgttattca
    13561 tgggttcgtc aatcaaatca ggcacaaggc tgccatattc ccgtcgcata acccggcttg
    13621 ccagcggggt ggtcaggatg tccctgactg actgtttcag gtgctccata tcgttcaggt
    13681 ttcccgttcc gtccggattc attcctgtgt agcgggttgt cactgcgggc ctcctgtcga
    13741 atcgctgcca cctttaacgc caccgtgctt atgcgtatgc actgtgatgc cgtttgaggt
    13801 gaaatcgccg ccactgtgcg tgatattgcc gctcatcttt cccccttttg tgacgtcaat
    13861 ctcggctgtt ttcagaaggt ttgtgcattc cacgacgggc gtatccagtt tcacgctgac
    13921 ggatgcctgc aaagtggccg ttttcatgcc gctggcactc agtgcgcctg cgtccgcgtc
    13981 gtagcggaac accgcgccgt ccggcgcgct gaccacgatt tctttcaggc ttttgccggg
    14041 tgccggactg gcatcactcc acaggctgcc aattatcatg gcggtttccg ggttgccgcc
    14101 aatgcaggca attaccacct gttcgccggg tgatggcggc agccacacat tgaaggctcc
    14161 cgcgcgcgtg gtgttccagc gcaaccagcc tgtttccagt tcgccgctgc gaacgcgcac
    14221 gcaccaggac ttctcatcaa cttcagagat aatcccggtg cggatgatgt tgctcagcag
    14281 tcgcatgagt tctgcgctca ccgtacagcc tccgcaatcc ggcccagcac cgtgttataa
    14341 atcaggcgtt catctgcctg gctgataccc agcagctcac gtaccgggta atcggtgaaa
    14401 atgcccggcg caacctgatc gcgctcaccg aactgatgaa cgcgtgcaat acgtgcagcc
    14461 acgccgctgt aacccaccgt cacaccggaa gcatctgcac gggctttcag gtagcgggcg
    14521 gtgcgcagtt ttacgaacat ggggacgcgc tttgtgctgt cctggttgat gcgccgggtg
    14581 cgtatttcca gaaaacggtc gatgtcatcc cggtaaaacg tgcggatatt gtttttatcc
    14641 tcatcccacc cggtaatggt tcgcccgtat ttccccgtgt cgtgatgcca gtttttcagc
    14701 gtgcgtgctt cgttattcca gataaagcga atgcgctcct gtatccgggt tacgcggcgt
    14761 ctgcgtggtg tccatgcggt cccgtccggc gctttctgtg accggatgcg cgcctgctgg
    14821 gcgcggcgta aatcctgtgc cagctttctg gcgatgttat tgatggcctg ctgattcagg
    14881 ctgtcgcgga tggcctcaaa ggtttcatcc acgcgggtga atgccttatc catcgctttc
    14941 accccacgtc acatcctgga atacatgcga ccagtcgcct tcggaagagg gcagacgggg
    15001 ttttggctcc ggcaggtgtt ctgcctgcgg tgtcccctga ctgttgcgcg taatgcgaac
    15061 gcgttcccgc agggggagcg taaacaggag atcggcgctg tcatcgtcat tgataacggc
    15121 ggagaatttg atgtcctgat tacgctcagg gttgagcaac aactgtggct gattttcgga
    15181 taaccacgcc agtagcggca gcgtgaggtc gtccagctcc ccggcgtaat ccatgacaaa
    15241 catcaccatc tgatagcggt aaacaaacga gggcgtttct ccggtcgttt caatgttgcc
    15301 gctctccacg aaaatggtga atttttccgg gttggcctga caccatcggc atgaacgggt
    15361 catggcttca cgcagggaat cagttttcag catggttgtt gtcctcgttg ttcagtcgtt
    15421 gcagccggcg ctgttccagt aattcaatgg cccgtttatc cgcgttacag gtttccagtg
    15481 catccagaag gcggtcgccc catataccga gatttcccca tgtgggagta tcagggaagg
    15541 ggggaggcat taccggtatg gtcagcgtct gcggtataag ccggactgac ggcgctggcc
    15601 gtggcgcgtt ctgcgtgcct gcgcaacctg tcagtaaaac gagcgtcagg caaagcgtgg
    15661 gcgcattcat cttttgcaat atcgttgcgt agctgttcac gtctggcctc tccgtcctga
    15721 ttgcgttgct gattttccgc gcggagttgt gccagcacct gctgcatatc ctgtaccccg
    15781 gcgctgatga tattcagggt gtcggcggta cttttcaggg cgctggcctg cgcttcgttt
    15841 ctggcgttct cccgacccag cgaccacgac agacgcatgg atgttcccca tgcggcaatc
    15901 agaaggaaag cgacacccag cgtgggccag agcttcatgc cggataggct ccgtgtggta
    15961 actgaaaatg cggtccgtct ttcagggtct tccagtctcc gccccattcc accggaatat
    16021 tcagttcccg gctggcctgt ctgaatgctg ctgcgatttt ttcgtactgc ggccattccc
    16081 atgacacctg gctgccgaca taagccacaa catccacggc atgccccgta aggtggcggc
    16141 tgttcatggt ctggctctta cccgtggcca caagttgctt ctggcggtaa cggctgcgca
    16201 ccccttcggt gataccaaaa tccacttccg agatttccag tgcccgtcgg gtcactttca
    16261 ccagatcagg atttacaccc tgcaaattct tttcgctccg gctgctgaat ttaaatgtgt
    16321 tgctcattcg tccttctcct tcaccctgcg attaaaggcc gcaataacct tgtcgcgtgc
    16381 tttctctgcg cccataaaac cgattgatgc gccgataaac gtcacggcat cttcaggaaa
    16441 cccgaagaag cgcaacgacc cggccacggc catggcaaga acgccgcacg ccagcgatcc
    16501 cgttacggtc tgaaccagtg ttcgtccgtc ataaagactc atcagcgcgg aaatgctgac
    16561 tgccgcgcct actgcataca ccgttggcag gtggtcaaag agccacgcaa taacctgctc
    16621 tgtgatccct gtttgaatgg tgctcactgc tactcccccc acaactgaat catttctcgt
    16681 ttcttcttct ccggctccgg catctccact tcctgcccgg cgtccagaaa tacctgctga
    16741 cagagtccgg ggttggcatc cagcaccttt tcggtgacgc cctgcgtcgt gccgtagtac
    16801 cggaaacaga gcgaatccac ggtgtcgcct tccagtgcct tcactttcat cagcacagct
    16861 ccgcaaagat tcgcgggcgg cgcagaatgt cagagatggc ccagctcaca tcgcgccaca
    16921 aatccgatgt ctgtatatcc agtgcgtccg cccggcggtc gcccttatcc gttgtgtccg
    16981 catcgcggta acgctccaga atcagggcgc gtgtggcggt ataaacagca ttgcgccagt
    17041 gccagagatt gacgctttct ccgttaatta cgggggccgg aacatcggcc agcgtctgat
    17101 ggccagccgc ctgctgttcc tgctgccacg cttccagctc gcgggtaacg tgtgccacgg
    17161 ccccggtggc ggtatgcagc aggcgggagg tggtcacgcg gcccggcagt cgtatcgcca
    17221 gacgcagctc acgcagcaca atatccggcc agaatgcacc cgctgaaata cgggtatcac
    17281 catcatcggt atcggtgatg tcgtcctctg cgggtccggg gttggttctg gcaaccatac
    17341 tcatggggtt cactcctgaa aaaatcgggc ggtgggtgcg cggtgtaaac ggtcacggag
    17401 tcaaaccgga acaccgcgca cgccgcccgc tgacggggtc agtcgttaac cgcgcttcgc
    17461 cttctgcgtc gcggtggttt ttcgtgttgc aggcttccgc gttgtctttt tacttttgct
    17521 gctttcgtcc tgcgtctgcg gtgtgctggc atcttctggt gcggctgcgg aatcagcttt
    17581 tttcagggcg cgggaaaggg ttgcaatctc gcgtttcaca cctgcgttcg ggttcaggtg
    17641 catcgcttcg cgcagcagct tcagtgatga ggccatgctg tccgcatcgc tcaggccacg
    17701 gcgggcaaag gcgcacgcct tgcataattt ggcgcgcact tcgtccggca tatcctggtt
    17761 ggcgacaatt tcccaaagtg tgtccagtgg ttcgataaag gtggacaaat ccgcgtcggc
    17821 atccgtcccg gcctgcgtca ataccgggtt acagatttct tcggtcagta ccgtggcagc
    17881 agtacggcca aagttatccg gcatgatgag gttgtgacgg accacatacg caccaatacg
    17941 cagcgcaagc ggaagatcgc cgcagtcaat cgcccacacc atcagcgtgg caatcacttc
    18001 atcctgctgc ccgccgtcag cctccagcgt tccctcaatc cagccggaaa agtccggcaa
    18061 caactctttt ttgatggcgg ctttcgcgct tctggcctgt acgcccttaa gccgggcctg
    18121 tgccagacgc agacgataca gcacctcttc atgcgcggta cgcgcggcgt ggtccacgcc
    18181 ttcattcgcc cggcctgcgc gctgtgccat cacgttctgc cagtgttgct gtgcaggagt
    18241 aatcattttt tctctccgtt acaggcgggc atgatgcccg ccgtgagttg attagctgtc
    18301 ggcgaacttc aggccagtga ccatcgcgca cttgccatag tcttcaacga cataagcgtc
    18361 attgatggac tggtaggtgg cgatgcggtt gtattccggt tcgtctttca tcagacgacg
    18421 cattgaacct ttctgccagt aaatcgacag gttgttgaac gaggtgatca gcatcgttgc
    18481 atccgggaag aacggcgcaa ggaacacgcc cagcccgcca atggtgcgcg atgacaggat
    18541 gagctgcccg gcaagtaatt ccgcatttgg attctggccg ctgatgctgt tcagcacggg
    18601 cagacgcagc gagttaaaca ggttgcgccc cataatcacc acaaggtcgt cagcttcctt
    18661 gtgccattca tccagcaggg atgaacgcgc gtcctgtacc agtgcatcag cgttcgcata
    18721 cttacccgcg tgcgccacgg tgttgtccat gttgcgggag gtcagcgtca cgtcattcat
    18781 aacgcgttcg ctggcgtcgg ttctgatgtg ctccagccat cccacgttaa cgtcctgaag
    18841 cagcttgttg gtgctgaagt tggactcatc cgcgtgagac gtgccgttga aaccgatcat
    18901 gatgcggtca agcgccacct gtcgggcaat ctgtgcgctg atgcgggact gaaaatcagg
    18961 atgtgccgcc caggcatcaa gctgcggata tgaaataaac gtgtcgtagt tcacctgttc
    19021 gcactggtat ttgcggtttt tcagatcaac cacgttattc gggttacggc gttttgtgcc
    19081 gtcataactg gtattcgtgc gtgcaattgg cccggtggtg tccaggagga ttttttcgcc
    19141 tttctggtcg gtcacgccga tcacgttaat tctttttgta aattcggtgc tttcctttga
    19201 ggcgttttca aaacgctgct gcaccgaggg ttccacggta aatcgcgata ccagtgcgga
    19261 aaccggaata ttattaagcg acgcctgctg cgccatatag caacccagct tgttgcgggt
    19321 aatatctgac atcaccagat tcataaaaaa tttgctcctt tgtcttatca gaagtcagcc
    19381 agctggtcgg aggctgcacc tgttgcggtg aaccggttct gcggatcgcc gtcctgcgtg
    19441 cgcagttttt ccttcagtgc tgccagctct gtggtcagtg acgtaatttt ctggcggtcc
    19501 tgctgatggc gggtttccag cacattaaaa cggtcgataa tgtcggcctg tgacgttgcg
    19561 acgccttcca ccgcttcctg aatacgggaa aaactggcgt catccgcttt gcggccacgg
    19621 ccaataatcc ccattacgcg gttaaaccac tgggtgcctt cttcctggcg ttgttctgcc
    19681 atttcgatga tttcagactc gatggcttcg gagataagcg gcgcttcacc ctggatactg
    19741 ttgaacgtca tcaccgcctg acgttgctgt gccgtgaatt tcaggcgctc agtgcccagg
    19801 cttgccgggg tgtcggtcat cgccagcccg accagatagg cgcgcccgtt aacggagaac
    19861 tgcgggtgca gttcgatact ggaatagatt ttcttgccgt ccgcgacaag ctgcttcatg
    19921 cgctcggtcg gttcgatttc tgcatacagc gcagtacgtc cggccagcgg accttccgta
    19981 atgtcttccg tactcagtgc ggtgacatcg cccattgcgg aaaattcgct tgacgggcat
    20041 ggcgagagat agtgctcaac gttcacgcgg gcagcgtaaa catccgggtt gaagttctcg
    20101 gcggcttcac gcagatgcac cggactgatt tcacggccat caacagttga tccggagaca
    20161 gccacgcgaa actttttgcg ggatgtcttt ttttcattag ccatagtttt tgcccctctg
    20221 actggttctt cagtcatgat ggcaaagcgt aacaggctga tacaaagggc ttttgttgta
    20281 agaaaacagt cagaacagag ggttaaggag aacagtttcg cgcgcgggta atcttcctgt
    20341 aattactcag ggggagcaat gattcaggac gcttttgtgc gccagcgtgc gcggcaactt
    20401 tactggcagg gttatccgcc cgcagaaata tcacgtctga tgggaataaa cccgaacacg
    20461 atttatgcgt ggaaaaaacg tgaccagtgg gatgaaacgc cacccgtgca gcgtgtcacg
    20521 cagtccatcg atgcgcgcct catccagctt actgaaaaac agaataaaac aggcggtgac
    20581 tttaaggaaa tagacctgct gacccggcag cttaaaaagc tgcatgatgg ccagccggat
    20641 gtgatggccg caggaaagaa aggccgggcg aaaaaactca aaaatcattt cacgccgaaa
    20701 cagattgccg cactgcggga aaaaatcatc agcaggctgg agtggcatca gcggggctgg
    20761 tttgactccc tgaccctttg cagggaagca gggatacgta acaggatgat cctgaaatcc
    20821 cgacagattg gggcgacctg gtattttgca caggaagctc tgctgatggc gctgcgtgac
    20881 gatgtggcgc aaccttacca gcgtaaccag atttttttgt ctgcgtcgcg tcgtcaggcg
    20941 ttccagttta aaagcattat tcagaaggcc gcggctgaag ttgatgtgga gctgaaaggg
    21001 ggcgataaaa tcatcctctc caacggcgca gagctgcatt ttctcggcac ttctgctgcg
    21061 tcggcacagt cctatacggg caatttttat tttgatgaat tcttctgggt cagtcgcttt
    21121 gctgaactgc gcaaggtggc tggcgctatg gcaaccctca gcggactgcg gcgcacctac
    21181 ttctccacgc catccaccga aacgcacgag gcatacgcct actggaacgg cgaccgctgg
    21241 aacgagaaaa aggccacgca taaacgccag cgtttttctg tggactggaa aacgctgcat
    21301 aacgggctta tctgccctga ccggacgtgg cggcaaattg tcacgctgga agatgtggtt
    21361 aatcacggct ggaaacacac cgatattgat gaaattcgtg atgaaaacac cgaagacgag
    21421 ttcctcaatc tctatatgtg tgagtttgtt cgcgaagggg aatcggcatt taacctgaat
    21481 atcctgattg gctgcggtgt tgacggatac gacgactgga aagactggaa accttttgct
    21541 ccccgcccga tggggaatcg tccggtatgg attgggtatg acgcaaacgg cagcagtggc
    21601 aacggcgaca gcggcgctgt gtccgtggtg gttcctccgg ctgttcctgg tggccgtttt
    21661 cgaacggtgg agacgcgacg cgttcagggg ctggagtttg aagaacaggc cagagtcatt
    21721 gaagagttca cgcgtcgcta caacgtggaa cacatcggca ttgatgtgac gggcgggaac
    21781 ggggaggctg tttatcagat agtgaagcgg tttttccctg ctgctattcc gtacaccttc
    21841 acgctgtcat ccaaacggtc gctggtactg aaaatgctgc aaataatgcg tgccggacgg
    21901 tgggaatacg atcgcgccga acgcgagctg gtcgcggcct ttaacgccgt gcgtaaggtg
    21961 aaaacaccgg gcggctttat cacttacgaa acggaccgcg cgagggggat cagccacggc
    22021 gaccttgcgt gggcaaccat gcttgctgtc attaacgaac cgattggcgg cgaaggagaa
    22081 aacgagcgtt tcacggttat ggagttctga tgagcagaaa aaataaaaaa gtgcgcatga
    22141 gttcacgcat tgatctcgct gatgcgctca ggaaagaatc atcgctcagt gcattcacat
    22201 ttgatggtcc ttatcgcctg accgggcatg acctgctgga caatatgtac tgtgctgata
    22261 acgggcggtg gtatgaaacc ccggtggact ggtacggtct ggcaagagca gcccggcaaa
    22321 cgtcctggca tcagtctgcg ctttacttta agcgcaatgt attactcggt tgctacatcc
    22381 cgcacccgct gctttcccgg caggatttct cggcgctggc gctggactgg tttgtgttcg
    22441 gtaacgcatt ccttgagctt agaagcaata tgctcggcga accgcttaaa ttacggcacg
    22501 cactggcgaa atacatgcga cgcggaagcg atcttgaatc atggtggtat gtgcaggatg
    22561 gcaaggacgc gttccagttt cgccctggca aagtgtgcca cctaatgaat ccggatatta
    22621 accaggaaat ttacggcatg ccggaatatc ttggcgcatt actctcggcc agcctgtctc
    22681 attcggcgga catgttcaga aaactgtatt acgacaatgg atcccacgcc gggtgcatca
    22741 tctacatcgg tgcagcgcag gtaaaccgcg aaagcatgga ctccctgaaa gaaacgttac
    22801 agggtgcgcg tggtggtggc gcgtttaaaa acgtgctcat ccatgcgccc aacgggggca
    22861 aagagggggt gcaaattttg ccgttccagc agatcaccgc aaaggatgag ttcatgaatg
    22921 ttaaggcggc atcccgtgat gatgtgctgg ctgcgcaccg cgttccgccg caactgatgg
    22981 gggcgatgcc gggcgaaaaa agtgcgtttg gtgatgtgga gaaggccgcg cgggtttacg
    23041 caattaacga gctgatgccc gtcatggagg ccatgaagca catcaatgac tggcttgggg
    23101 aagaggtgat ccgctttaac ccttacgcac tgttagacac ccagcccaca tcctgacgcg
    23161 cttcgcttgt ctgctgcttc gccggggcat aaaaaattta tgccccgact ctccagctcc
    23221 tgtatcagtc agataatttc acgacgcttt cctgctgatt gccatcatcg acagtcagac
    23281 tcttacgcaa tcccaccgcg ttgactgcat gttctcgccg tctcagtgcg attttgacgg
    23341 ccttaccttt caccccatca aatcaaaagc cctcacgtct ttttcacgct cagcgtgaga
    23401 aatacagcca ttctgttgta tctctgcgac atcgttcagg gaatgctatt taccccctga
    23461 aacgcgggct gttcccccgt cacctgcgcg cagaaaaaac gcgttttttt gtgcacgcac
    23521 ggatccttga cggatccagc cgccacgggg gccggaaggg caaaaagtcg ttcaaaaaaa
    23581 ttgtgcaaat ttgtgcacta tcgtgcagtg tgcatcgtgc tgttttctgc gcaaaaaacg
    23641 gatggtaaat ttgttcgtag cagggaaatt ttaatgggcg caagacatga agaaagattc
    23701 atatccttat ttgatttgca tgactgtttc agtgctaggg attattttcc tcttttactg
    23761 gtggagggca gatatctaca ggattacata tcttaattcg cgtatatcca attattacat
    23821 cttgtgtagt atgggtatgt cctttttctt ctccttgttt ctggtgaaga ggggggtagt
    23881 gaagcaaagt ggctggaaag gactgtcgga atacctgaag gtttatgctg gctcttgcat
    23941 tcttacaggg atattcctga ttatccctct tatgatggtg acttattttt taccaggagt
    24001 aacatcatca tatgttgcac cgtatgagta tacatctggt agttcaagaa gctgttccgg
    24061 agcgtttgtt gatgatcccg aactgcataa aaatattctt atttgctatc cgtatggt
//
LOCUS       DAZFXT010000071        23641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.071, whole genome shotgun sequence.
ACCESSION   DAZFXT010000071 DAZFXT010000000
VERSION     DAZFXT010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.071"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(84..2153)
                     /gene="glyS"
                     /locus_tag="ACHKW0_002994"
     CDS             complement(84..2153)
                     /gene="glyS"
                     /locus_tag="ACHKW0_002994"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HFO7475793.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2163..3074)
                     /gene="glyQ"
                     /locus_tag="ACHKW0_002995"
     CDS             complement(2163..3074)
                     /gene="glyQ"
                     /locus_tag="ACHKW0_002995"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HFO7475794.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3169..3468)
                     /gene="ysaB"
                     /locus_tag="ACHKW0_002996"
     CDS             complement(3169..3468)
                     /gene="ysaB"
                     /locus_tag="ACHKW0_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HFO7475795.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3643..4638
                     /gene="wecH"
                     /locus_tag="ACHKW0_002997"
     CDS             3643..4638
                     /gene="wecH"
                     /locus_tag="ACHKW0_002997"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HFO7475796.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4680..5117)
                     /gene="yiaA"
                     /locus_tag="ACHKW0_002998"
     CDS             complement(4680..5117)
                     /gene="yiaA"
                     /locus_tag="ACHKW0_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HFO7475797.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDTGINPPKETQVTQEEYSE"
     gene            complement(5163..5504)
                     /gene="yiaB"
                     /locus_tag="ACHKW0_002999"
     CDS             complement(5163..5504)
                     /gene="yiaB"
                     /locus_tag="ACHKW0_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaB"
                     /protein_id="HFO7475798.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5673..7127)
                     /gene="xylB"
                     /locus_tag="ACHKW0_003000"
     CDS             complement(5673..7127)
                     /gene="xylB"
                     /locus_tag="ACHKW0_003000"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HFO7475799.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7199..8521)
                     /gene="xylA"
                     /locus_tag="ACHKW0_003001"
     CDS             complement(7199..8521)
                     /gene="xylA"
                     /locus_tag="ACHKW0_003001"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HFO7475800.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAGKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8887..9879
                     /gene="xylF"
                     /locus_tag="ACHKW0_003002"
     CDS             8887..9879
                     /gene="xylF"
                     /locus_tag="ACHKW0_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7475801.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9957..11498
                     /gene="xylG"
                     /locus_tag="ACHKW0_003003"
     CDS             9957..11498
                     /gene="xylG"
                     /locus_tag="ACHKW0_003003"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015614 - ABC-type D-xylose transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015753 - D-xylose transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HFO7475802.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11476..12657
                     /gene="xylH"
                     /locus_tag="ACHKW0_003004"
     CDS             11476..12657
                     /gene="xylH"
                     /locus_tag="ACHKW0_003004"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HFO7475803.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12735..13913
                     /gene="xylR"
                     /locus_tag="ACHKW0_003005"
     CDS             12735..13913
                     /gene="xylR"
                     /locus_tag="ACHKW0_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="D-xylose enhances binding of XylR to the xyl
                     promoter and activates transcription; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose utilization transcriptional activator
                     XylR"
                     /protein_id="HFO7475804.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEIML
                     "
     gene            complement(14021..14845)
                     /gene="bax"
                     /locus_tag="ACHKW0_003006"
     CDS             complement(14021..14845)
                     /gene="bax"
                     /locus_tag="ACHKW0_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HFO7475805.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15165..17195
                     /gene="malS"
                     /locus_tag="ACHKW0_003007"
     CDS             15165..17195
                     /gene="malS"
                     /locus_tag="ACHKW0_003007"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HFO7475806.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17373..18626
                     /gene="avtA"
                     /locus_tag="ACHKW0_003008"
     CDS             17373..18626
                     /gene="avtA"
                     /locus_tag="ACHKW0_003008"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HFO7475807.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPEDRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18778..19251)
                     /gene="ysaA"
                     /locus_tag="ACHKW0_003009"
     CDS             complement(18778..19251)
                     /gene="ysaA"
                     /locus_tag="ACHKW0_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7475808.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVNAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19353..20201)
                     /gene="yiaJ"
                     /locus_tag="ACHKW0_003010"
     CDS             complement(19353..20201)
                     /gene="yiaJ"
                     /locus_tag="ACHKW0_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HFO7475809.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFNIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DQGAIT"
     gene            20402..21400
                     /gene="yiaK"
                     /locus_tag="ACHKW0_003011"
     CDS             20402..21400
                     /gene="yiaK"
                     /locus_tag="ACHKW0_003011"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047559 - 3-dehydro-L-gulonate
                     2-dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HFO7475810.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21412..21879
                     /gene="yiaL"
                     /locus_tag="ACHKW0_003012"
     CDS             21412..21879
                     /gene="yiaL"
                     /locus_tag="ACHKW0_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HFO7475811.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKALDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21997..22470
                     /gene="yiaM"
                     /locus_tag="ACHKW0_003013"
     CDS             21997..22470
                     /gene="yiaM"
                     /locus_tag="ACHKW0_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HFO7475812.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22473..>23641
                     /gene="yiaN"
                     /locus_tag="ACHKW0_003014"
     CDS             22473..>23641
                     /gene="yiaN"
                     /locus_tag="ACHKW0_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter large
                     permease YiaN"
                     /protein_id="HFO7475813.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKF"
BASE COUNT         5876 a         5986 c         5812 g         5967 t
ORIGIN      
        1 cagaagatta ttaaaggttg ctgtattatt tagcgataat aaaaaaagcc tgccagatgg
       61 caggcttttt aataacggcg ttattactgc aacagcgaaa tatccgcaac gcgcaggaac
      121 agttcgcgca gtttctccag catggtcaga cggttgagac gcaattcttt gtcatcaacc
      181 atgaccatca ctttatcgaa gaaagcatca accggctcac gcagctctgc cagttcgacc
      241 agcgcatcct ggtaacgacc ttccgcaaag tacggctcca gcttgtcacg tagcaccaca
      301 acctgcatcg ccagtttaat ttcttccggc tctttcaggg tagaggcatt cacgcggtcg
      361 ctcagcactt cgtcagattt cgccaaaatg ttggagacac gcttgttcgc cgccgccagt
      421 gcagcagctg cttcaagggt acggaagtgc gataccgctt tcatgcgggc atcgaaatca
      481 gccggacgag tcggacgacg cgccagtacc gcctggatgg tgtcaacggt gtaaccttcg
      541 tcctgatacc aggcgcggaa gcgaccgagc ataaagtcga taacatcatc aaccacgttg
      601 gcgttagtca gcttatcgcc atacagacgc actgcttctt cggtcagcgt ttgcagatca
      661 aggttgaggt tcttctcaac gataattcgc agcacgccaa gtgcggcacg acgcagcgca
      721 aacgggtctt tgtcgccttt cggatgctgg ccgataccga agatacccgc cagagtatcc
      781 atcttgtcag caatcgccag cgcacaggct accgggttag acggcaggtc atcaccggca
      841 aagcgcggct gatactgctc attcagcgcc accgcgacat cttccgcttc gccatcgtga
      901 cgcgcatagt gcatccccat aacgccctgg gtgtcggtga actcgaagac catgttggtc
      961 atcaggtcac acttagacag caggcccgca cgggttgcgt ggttaacgtc agcgccaatc
     1021 tgttcagcaa tccagccagc cagcgcctgg atgcggtcag ttttgtcgcg cagcgtaccc
     1081 agctgttgct ggaacagcac ggtttgcagg cgcggcaggt tatcttccag acgtttttta
     1141 cggtcggtat tgaagaagaa ctcggcatcc gccagacgtg gacgaacgac tttctcgtta
     1201 ccagagataa tttgctgcgg atctttcgat tcgatgttgg caacgaagat gaagttcggc
     1261 agcagtttgc catcgttcgc atacaccggg aagtatttct ggtcaccttt catggtgtaa
     1321 accagggctt cagccggcac cgcgaggaat ttctcttcga attttgcggt cagaacgacc
     1381 ggccactcca ccagcgaagc cacttcttcc agcaggcttt cgcttaagtc agcgttaccg
     1441 ccaatcttac gcgctgcttc ttcggcatcg gctttaatct tcgctttacg ttcttcgtaa
     1501 tcggcgatga ctttcccgcg ctcacgcaga atttccggat actgatcggc gttgtcgatg
     1561 gtgaattccg gttcgcccat aaagcggtgg ccgcgaatca cgcgatcgga ctgaatgccc
     1621 agaatggttg ccggaatgac tttgtcgccc agcagcaggg tcacggtgtg caccggacgc
     1681 acgaagtgca cgtcgcttgc gccccaacgc atcagtttcg ggatcggcag tttcgccaga
     1741 gaagtcgcaa ccatattcgg cagcagtgct tcggtgcttt cgcccttcac atgggcgcga
     1801 tacagcagcc attcgccttt atcggtagtc agacgctcag cctggtcaac ggtaataccg
     1861 caaccacgcg cccaaccttc tgccgctttg ctcggtttgc cttcagcgtc gaacgcctgg
     1921 gcaatcgccg ggccgcgttt ttcgatttca cgatccggtt gcgcttccgc caggttagct
     1981 actttcagcg ccagacgacg cggagcagca aaccattgaa cggtgccgtg tgcgaggcca
     2041 gcgttatcca gctccgcagt aaaattcgca gcaaaggact cagccaggct gcgcagtgct
     2101 tttggtggca gctcttcagt gccgatttcc accagaaaag ttttctcaga catagccgcc
     2161 tcttacttat ctttgttgca catcgggaag ccgagggctt cacgggaagc gtagtatgct
     2221 tctgccactg ctttggtcag ggtgcgaatg cgcagaatgt agcgctgacg ctcggtgacg
     2281 gagatggctt tacgcgcatc cagcaggttg aagctgtggg cggctttcag aatacgctcg
     2341 taggctggca gcggcagcgg attttccagc gccagcagct gctgcgcttc tttctcgtac
     2401 tgctcaaagc aggtgaacag gaagtccaca tccgcgtatt cgaagttgta agtggactgc
     2461 tccacttcgt tctgatggaa cacgtcgccg taggtggttt tacccagcgg gccgtcgctc
     2521 cagaccaggt cgtaaacgct gtctacgccc tgaatgtaca tggccagacg ttccagaccg
     2581 taggtgatct cgccggtaac cggtttacac tccagaccac caacctgctg gaagtaagtg
     2641 aactgcgtca cttccatgcc gtttagccac acttcccagc ccagtcccca ggcacccaga
     2701 gtcgggtttt cccagttatc ttccacgaaa cggatgtcgt gaatagtcgg gtccatgccc
     2761 agctctttca gagaaccgag gtacagctcc tgaatattgt ccggcgatgg cttaatgacc
     2821 acctggaact gatagtagtg ctgtaaacgg ttggggtttt cgccgtagcg accatcggtc
     2881 gggcgacgag aaggctgaac ataagcagcc gccatcggtt ctggccccag cgcgcgcaga
     2941 caggtcattg ggtgagaggt tcccgcgccg acttccatgt ccaatggttg aacaatggtg
     3001 cagccctggc gagcccagta atcctgtaag gtcaggatca agccctggaa ggtcctggta
     3061 tcaaactttt gcatattatt tcgtgctgga tacgtgtgga tttaaaggaa gggatcagta
     3121 tacccgctgg atggaagata tacagtacga aacgggaaaa agcagggctt aacgcatgga
     3181 aagatgcaaa aaatggccat ctgcgtcaaa agaacagaca aactgctctt tacggaaggt
     3241 ataaccgcgc atttcatagc ttccctggaa ctgttcgaag gcggtgacgt cgattttttg
     3301 ctcgccggtg ttataacgtt ttgccgcctg atccttgcat aacgcttcca tattcaacga
     3361 acgcagagga tcaaccttta cccgttgtgc tttctgcacg ggtgacggtg tagaacaacc
     3421 cactagcacc ataagcgcca ttgccggaaa gaaagcgttc atcatcatta aattaccgtc
     3481 tggtttgcag gtgagtctta ttatttatat cggtatagtg gctgatatct tacgaatttc
     3541 cttaagcctc tataagctgt agtgataaaa ctcagattat tccagtgcgc caactgaatg
     3601 atttatcggt cagacttttt ttaaccagat acagagacac agatgcagcc caaaatttac
     3661 tggattgata acctgcgagg gatagcgtgt ttaatggtgg tgatgattca caccactacc
     3721 tggtatgtga ccaatgctca tagtgttagc cccgtcacct gggatatcgc caatgttctg
     3781 aattctgcct ctcgtgtcag cgtgccgcta tttttcatga tttccggcta tctctttttt
     3841 ggcgaacgca gcgcccagcc gcgccatttc ttgcgtatcg gcttatgtct gattttttat
     3901 agcgcaatcg cactgctcta cattgcgctg tttacctcca tcaatatgga gttagcgctg
     3961 aaaaacctgc tgcaaaagcc agtgttttac cacttgtggt ttttcttcgc gattgcggtg
     4021 atttatctgg tttcaccgct gattcaggtg aagaacgtcg gcggaaaaat gttgctggtg
     4081 ctaatggtgg tgattggcat tatcgctaac ccaaacacag tgccgcagaa aattgacggt
     4141 tttgaatggc tgccaattaa cttatatatc aatggcgata ctttttacta cattctgtat
     4201 ggcatgttgg gccgcgctat agggatgatg gacacacagc ataaagcact gtcgtgggtg
     4261 agcgccgcgc tgtttgcgac gggggttttt attatctctc gcgggacatt atatgaattg
     4321 cagtggcgcg gaaattttgc cgatacctgg tatctttact gtgggccgat ggtttttatc
     4381 tgcgcaatcg cgctattgac tctggttaaa aacacgctgg atacgcgtac cattcgcgga
     4441 cttggcttaa tctcccgcca ttcattgggt atatacggat tccacgcctt gattatccat
     4501 gcgctgcgca cccggggaat tgagcttaaa aattggccaa tactggatat tatttggatc
     4561 ttttgcgcga cgttggcagc gagtttgtta ctttctatgc tggtacaacg aatcgacaga
     4621 aacagattag tgagttaagt aaaagcccgt tcacattgga ctgaccgggc ttacgtgaat
     4681 tattcgctgt attcttcctg ggtaacctgt gtttcttttg gcgggtttat tccggtatca
     4741 cgaatattct tctgcaccgc tactgcacca aacaagctta agaagaaagc cagtccataa
     4801 aaaccttttt cactgagtaa taatgtcgca ttccacagac ctaccatcag taatgcaaca
     4861 gagataataa agacagtcag gcaggtcata taataaatgg aagtggtcgg tatgccttca
     4921 tacttgtccc gaacggtctt ttgataagac gcagcagaaa acagtcctaa caccagtacg
     4981 gcaaaataat accctttttc atttaactgc atctctgcat tccatagccc taacagatag
     5041 gtaacgatac caccaacgag agctatccat gacacaatac taaaggccgg tgaataggtt
     5101 gatattttgt tgtccatcac agtattcctt tcattcctga atgtgtaaga gctttatgtt
     5161 gctcataccg agcgaaacaa acgcatttga ccaaacaagc agacaaaaaa ggcgatgggg
     5221 tagatcacca tttcataagc attgataggt gcatttaaaa caccgataaa caagagaccg
     5281 atagtgatta acgcaactaa ctggcagata tgggtaaaaa aattatccag ttgcccgagt
     5341 ttttctgcgc gaagatatgc atagttacca aaagttgctg tcattaacac gccaagaaaa
     5401 tagccttttc cacttagcaa tgggcatgag atccatagcc caaccagata aaccagcgcc
     5461 ccgacaacaa ataatagttt tgccacagtt tttgaggttt tcataacgat ctccatatct
     5521 accagcgata cattacgagt aaccaacgaa agacaaaact gaaaaatgcc attaacaaat
     5581 gattttcaga ataaattcat actaaatatt aattaattag tgagatatat agatgtgaat
     5641 tatcccccac ccggtcaggc aggggataac gtttacgcca ttaatggcag aagttgctga
     5701 tagaggcgac ggaacgtttc acgtcgtggc tgataagcgg cataacgctg cgcatctggt
     5761 agatgcgact gttctaacgg tagctgcggc aacaactcaa tgagcgattt ctctggattc
     5821 gccgcaatct gcgccagcct tgctgcgccc agtgctggcc ccacatcccc ccccgtacgg
     5881 taatcgagct gctgaccgct gatatccgcc agcatctgac gccagtactc actacgcgcc
     5941 ccgccaccaa tcaacgtaac actttgcggt ttaatgccgc aggcatgcac gacatccatg
     6001 ccatctgcca gcgcataacc cacgccttcc agcactgctc gcgccagttc attggggcca
     6061 tgttgatgag tcaaaccaaa gaaaaccccc ttcgcctggg gattattgtg tggtgtacgc
     6121 tcgccggaaa gataaggcag aaaccaaact ggctcggcac tttcatcagc ctgttgagct
     6181 gcagcgatta aagctgggac attgcacagg ccggttaatt tcgcgaccca atccagacac
     6241 gatgctgcac tcagcattac agacattaaa tgccaacgct gcggtagcgc atggcaaaag
     6301 ctgtgtacag cgctttctgg cttgcttaag aacccttcgc tgacagcaaa atagaccccc
     6361 gacgtcccca gcgataacat tgcctgatta gcatcaacca ttcccacacc aactgcacca
     6421 gctgcattgt cgccaccgcc tgcgacaact ggcaccgtcg ccatacccca cgctttcgca
     6481 acttcaggta acaaagcacc agtaatttcg ctgccttcat ataatgcggg catctggtca
     6541 cgagataagt cgcaagcctg cagcatgacg tcactccagt cacgctttgc gacatccagc
     6601 cacatggtgc cagctgcgtc agacatatcg ctggcaaact cccccgtcat acgcagacgc
     6661 aagtaatctt tcggtaataa tactttgtcg atttgacgga atatctccgg ctcatgccgc
     6721 tgaacccata gcaatttagg cgcagtaaat ccgggcatca tcaggttgcc ggtaatcact
     6781 cgtgattgcg gaactctcgc ttccagcaaa gtgcactctt gcgcacagcg cccgtcgttc
     6841 cacaaaatgg cagggcgcaa tacccgttgt tgagcatcca gtaaggttgc tccatgcatc
     6901 tggccggcaa tacccaatgc tttaacgtcc tgcagagaat gctgatcgcc cagagctttc
     6961 attgcgcgat cagttgcctg ccaccactgt tccgggtctt gttccgacca gagtggatgc
     7021 gggcgcgaaa cggtcagctt ttccgtttgc gaagcaacca cctcaccctg ctcgttgagc
     7081 aaaataactt ttacgcccga ggtgccaaga tctatcccga tatacatatc gatcgttcct
     7141 taaaaaaatg cccggtatcg ctaccgataa ccgggccaac ggactgcaca gttagccgtt
     7201 atttgtcgaa cagataatga tttaccagat tttccagttg ctcctggcga ccactctgat
     7261 gcaccggaga caaattatgt tcctgagcat atttggctaa atctgccagt gacatttggc
     7321 ctttcaggat ttgctggccc aattcgctat tccagccgga ataacgctgc gcgatgcgtt
     7381 tatccagctc gccatcttca atcatgcgcg ctgcaatttt cagcgccagt gccatcgtat
     7441 ccatcgcgcc gatatgaccg taaaacagat catatttatc agtactttga cgacgtactt
     7501 tggcatcgaa gttcagacca ccggtggtga aaccgcctgc tttgagaatt tcatacatca
     7561 ccagcgcatt ctcttccaca ctgttcggga actggtcggt gtcccagccc agttgcgcat
     7621 cgccacggtt ggcgtcgaca gaaccgaaca ggccaagcgc aatggcggtg gcaatttcat
     7681 gatggaaaga gtgacctgcc agcgtcgcgt ggttagcttc aatgttcagt ttaatctctt
     7741 tttccagacc aaactgtttc aggaagccat agaccgtcgc ggcatcgtaa tcatattgat
     7801 gtttggtcgg ttcttgcggt ttcggttcga taagcaacgt gccctggaaa ccgattttat
     7861 gtttatgctc aaccaccatc tgcataaagc ggcccagctg ttcacgctcc tgacgcaagt
     7921 cggtatttaa cagcgtttcg taaccttcac gaccgcccca caggacatag ttttcaccgc
     7981 ccaatttatg ggttgcttcc atcgctgtaa caacttgcgt tgccgcccaa ctgaagactt
     8041 caggatctgg gttcgtcgcc gcacccgcgc catagcgagg gtttgtaaag cagttagcgg
     8101 ttccccacag cagcttcacg ccgctctctt cttgcttgcc tgccaggaca tcaaccattt
     8161 gcgcaaaatt attgatgtac tcttttaacg acgcgccctc aggggaaaca tccacatcgt
     8221 ggaagcaata aaatggcaca tgtaacttgt ggaaaaactc aaatgcgaca tctgctttac
     8281 gcttcgccaa cgccagtgcc tcaccaggct gctgccacgg acgattaaac gcccccacac
     8341 caaacatgtc cgccccgttc cagcagaatg tgtgccagta gcaggcggca aaacgcaagt
     8401 gctcttccat acgcttgccc aacaccagtt cgtcgggatt gtagtgacgg aatgctaacg
     8461 ggtttgagga ttttgagcct tcataacgaa cgcgatcgag ctggtcaaaa taggcttgca
     8521 tattgaactc cataatcagg taatgccgcg ggtgatggat gatgtcgtaa tattgggcac
     8581 tccctttcag ttgctcaatt atgttatttc acactgctat tgagataatt cacaagtgtg
     8641 cgctcgctcg caaaataaaa tggaatgatg aaactgggta attcctcgaa gagaaaaatg
     8701 caataagtac aattgcgcaa caaaagtaag atctcagtca taaatcaaga aataaaccaa
     8761 aaatcgtaat cgaaagataa aaatctgtaa ttgttttccc ctgtttagtt gctaaaaatt
     8821 ggttacgttt atcgcggtga ttgttactta ttaaagctgt cctctaacta cagaaggccc
     8881 tacaccatga aaataaagaa cattctactc accctttgca cctcacttct gcttaccaac
     8941 gttgcggcac acgccaaaga agtcaaaata ggtatggcga ttgatgatct ccgtcttgaa
     9001 cgctggcaaa aagatcgaga tatttttgtg aaaaaggcag aatctctcgg cgcgaaagta
     9061 tttgtacagt ctgcaaatgg caatgaagaa acacaaatgt cgcagattga aaacatgata
     9121 aaccggggtg tcgatgttct tgtcattatt ccgtataacg gtcaggtatt aagtaacgtt
     9181 gtaaaagaag ccaaacaaga aggtattaaa gtattagctt acgatcgtat gattaacgat
     9241 gcggatatcg atttttatat ttctttcgat aacgaaaaag tcggtgaact gcaggcaaaa
     9301 gccctggtcg atattgttcc gcaaggtaat tacttcctga tgggcggctc gccggtagat
     9361 aacaacgcca agctgttccg cgccggacaa atgaaagtgt taaaacctta cgttgattcc
     9421 ggaaaaatta aagtcgttgg tgaccaatgg gttgatggct ggttaccgga aaacgcattg
     9481 aaaattatgg aaaacgcgct aaccgccaat aataacaaaa ttgatgctgt agttgcctca
     9541 aacgatgcca ccgcaggtgg ggcaattcag gcattaagcg cgcaaggttt atcagggaaa
     9601 gtagcaattt ccggccagga tgcggatctc gcaggtatta aacgtattgc tgccggtacg
     9661 caaactatga cggtgtataa acctattacg ttgttggcaa atactgccgc agaaattgcc
     9721 gttgagttgg gcaatggtca ggaaccaaaa gcagatacca cactgaataa tggcctgaaa
     9781 gatgtgccct cccgcctcct gacaccgatc gatgtgaata aaaacaacat caaagacacg
     9841 gtagttaaag acggattcca caaagagagc gagctgtaag cgttacgccc cggcgcggag
     9901 cgggggcgtg atttctctcc atgccgcgtg aatgaattgg cttaggtgga gtcgttatgc
     9961 cttatctact tgaaatgaag aatattacca aaaccttcgg cagtgtgaag gcgattgata
    10021 acgtctgctt gcggttgaat gctggcgaaa tcgtctcact ttgtggggaa aatgggtctg
    10081 gtaaatcaac actgatgaaa gtgctgtgtg gtatttatcc ccatggctcc tacgaaggcg
    10141 aaattatttt tgcgggagaa gagattcagg cgagtcacat ccgcgatacc gaacgcaaag
    10201 gtatcgccat cattcaccag gaattggccc tggtgaaaga attgaccgtg ctggaaaata
    10261 tcttcctggg taacgaaata acccacaatg gcattatgga ttatgacctg atgacgctac
    10321 gctgtcagaa gctgctcgca caggtcagtt tatccatttc acctgatacc cgcgttggcg
    10381 atttagggct tgggcaacaa caactggttg aaattgccaa ggcacttaat aaacaggtgc
    10441 gcttgttaat tctcgatgaa ccgacagcct cattaactga gcaggaaacg tcggttttac
    10501 tggatattat tcgcgatcta caacagcacg gtatcgcctg tatttatatt tcgcacaaac
    10561 tcaacgaagt caaagcgatt tccgatacga tttgcgttat tcgcgacgga cagcacattg
    10621 gtacgcgtga tgctgccgga atgagtgaag acgatattat caccatgatg gtcgggcgag
    10681 agttaaccgc gctttaccct aatgaaccac ataccaccgg agatgaaata ttacgtattg
    10741 aacatctgac ggcatggcat ccggtcaatc gtcatattaa acgagttaat gatgtctcgt
    10801 tttccctgaa acgtggcgaa atactgggta ttgccggact cgttggtgcc ggacgtaccg
    10861 agaccattca gtgcctgttt ggcgtgtggc ccggacaatg ggaaggaaaa atttatattg
    10921 atggcaaaca ggtagatatt cgtaactgtc agcaagccat cgcccagggg attgcgatgg
    10981 tacccgaaga cagaaagcgc gacggcatcg ttccggtaat ggcggttggt aaaaatatta
    11041 ccctcgccgc actcaataaa tttaccggtg gcattagcca gcttgatgac gcggcagagc
    11101 aaaaatgtat tctggaatca attcagcaac tcaaagttaa aacgtcgtcc cccgaccttg
    11161 ctattggacg tttgagcggc ggcaatcagc aaaaagcgat cctcgctcgc tgtctgttac
    11221 ttaacccgcg catcctcatt cttgatgaac ctaccagggg tatcgatatt ggcgcgaaat
    11281 acgagatcta caaattaatt aaccaactcg tccagcaggg tattgccgtt attgtcatct
    11341 cttccgaatt acctgaagtg ctcggcctta gcgatcgggt actggtgatg catgaaggga
    11401 aactaaaagc caacctgata aatcataacc tgactcagga gcaggtgatg gaagccgcat
    11461 tgaggagcga acatcatgtc gaaaagcaat ccgtctgaag tgaaattggc cgtaccgaca
    11521 tccggtggct tttccgggct gaaatcactg aatttgcagg tcttcgtgat gattgcagct
    11581 atcatcgcaa tcatgctgtt ctttacctgg accaccgatg gtgcctactt aagcgcccgt
    11641 aacgtctcca acctgttacg ccagaccgcg attaccggca tcctcgcggt aggaatggtg
    11701 ttcgtcataa tttctgctga aatcgacctt tccgtcggct caatgatggg gctattaggt
    11761 ggcgtcgcgg cgatttgtga cgtctggtta ggctggcctt tgccacttac catcattgtg
    11821 acgttggttc tgggactgct tctcggtgcc tggaacggat ggtgggtcgc gtaccgcaaa
    11881 gtcccttcat ttattgtcac cctcgcgggc atgttggcat ttcgcggcat actcattggc
    11941 atcaccaacg gcacgactgt ttcccccacc agcgccgcga tgtcacaaat tgggcaaagc
    12001 tatctacctg ccagcactgg cttcatcatt ggcgcgcttg gcttaatggc ttttgttggt
    12061 tggcaatggc gcggaagaat gcgccgtcag gctttgggtt tgcagtctcc ggcctctacc
    12121 gcagtagtcg gtcgccaggc tttaaccgct atcatcgtat taggcgcaat ctggctgttg
    12181 aatgattacc gtggcgttcc cactcctgtt ctgctgctga cgttgctgtt actcggcgga
    12241 atgtttatgg caacgcggac ggcatttgga cgacgcattt atgccatcgg cggcaatctg
    12301 gaagcagcac gtctctccgg gattaacgtt gaacgcacca aacttgccgt gttcgcgatt
    12361 aacggattaa tggtagccat cgccggatta atccttagtt ctcgacttgg cgctggttca
    12421 ccttctgcgg gaaatatcgc cgaactggac gcaattgcag catgcgtgat tggcggcacc
    12481 agcctggctg gcggtgtggg aagcgttgcc ggagcagtaa tgggggcatt tatcatggct
    12541 tcactggata acggcatgag tatgatggat gtaccgacct tctggcagta tatcgttaaa
    12601 ggtgcgattc tgttgctggc agtatggatg gactccgcaa ccaaacgccg ttcttgattt
    12661 tgataaaaat tttctcaaag ccggttacgt attaccggtt ttgagttttt gcatgattca
    12721 gcaggaaaag aaccatgttt actaaacgtc accgcatcac attactgttc aatgccaata
    12781 aagcctatga ccggcaggta gtagaaggcg taggggaata tttacaggca tcacaatcag
    12841 aatgggatat tttcattgaa gaagatttcc gcgcccgcat tgataaaatc aaggactggt
    12901 taggagatgg cgtcattgcc gacttcgacg acaaacagat cgagcaagcg ctggctgatg
    12961 tcgacgtccc cattgttggg gttggcggct cgtatcacct tgcagaaagt tacccacccg
    13021 ttcattacat tgccaccgat aactatgcgc tggttgaaag cgcatttttg catttaaaag
    13081 agaaaggcgt taaccgcttt gctttttatg gtcttccgga atcaagcggc aaacgttggg
    13141 ccactgaacg cgaatatgca tttcgtcagc ttgtcgccga agaaaagtat cgcggagtgg
    13201 tttatcaggg gttagaaacc gcgccagaga actggcaaca tgcgcaaaat cggctggcag
    13261 actggctaca aacgctgcca ccgcaaaccg ggattattgc cgttactgac gcccgggcgc
    13321 ggcatattct gcaagtatgt gaacatctac atattcccgt accggaaaaa ttatgcgtga
    13381 ttggcatcga taacgaagaa ctgacccgct atctgtcgcg tgtcgccctt tcttcggtcg
    13441 ctcagggcgc gcggcaaatg ggctatcagg cggcaaaact gttgcatcga ttattagata
    13501 aagaagaaat gccgctacag cgaattttgg tcccaccagt tcgcgtcatt gaacggcgct
    13561 caacagatta tcgctcgctg accgatcccg ccgttattca ggccatgcat tacattcgta
    13621 atcacgcctg taaagggatt aaagtggatc aggtactgga tgcggtcggg atctcgcgct
    13681 ccaatcttga gaagcgtttt aaagaagagg tgggtgaaac catccatgcc atgattcatg
    13741 ccgagaagct ggagaaagcg cgcagtctgc tgatttcaac caccttgtcg atcaatgaga
    13801 tatcgcaaat gtgcggttat ccatcgctgc aatatttcta ctctgttttt aaaaaagcat
    13861 atgacacaac gccaaaagag tatcgcgatg taaatagcga gatcatgttg tagcgatgat
    13921 gagaattgtc ggcgtcacat cagggaactc agcacagcca cctgatgcga gcaaaagcct
    13981 tatccgaccg acaaatcaga taaggctcaa ggaaatgcaa ttacatatgc gccgcgatta
    14041 accgttggtt atcctggtac attgcgaaca ggtagttatt ataactcttc cctttggtcg
    14101 agtagccctt cagcttgtga atcatcgctg tggcagtcac ttcctgatcc gctttacgca
    14161 gctgcgcacg cgatttacgg aacgaagagt aagccgggtg cgtattcagg ttagtgacat
    14221 aggcgctcac cgattctttg acagaactaa actgtgagta ccctttcact ttacccggcg
    14281 cattggtaca acgtcctttc atgcatttca tgccgaacag attgttattg ttgcgcgcca
    14341 gcttcgacgt tccccaaccg ctttctgctg cagccatcgt cgccaccata ctggtaggga
    14401 taatgtctac gcgttcaagc aaggtattcc acgggatttt tcgcgtatta ccagaccact
    14461 tcaccttgta gcgtttggcg atgtctttca gacgcgcacg ctcagcaggt gaccattggc
    14521 cctgatactg ttttgaaatc agccagttac gttctgcagt aatggctgca ttttggctgg
    14581 taatgtaagg cattacggtc cggagaaacg cctttttcct tggtgttccg gaagggtatt
    14641 ttcgcaaatc aggaagtgaa ctactctttg cactattgcg agaatactct tgtttactgc
    14701 ttacctgttt attactagct ttagttaagt gggacttttg actcgctgtt gttgtgtgcg
    14761 tcttcgctaa cacctcactc gaaaacacca gagtgagtaa cataagaatc atcgccccat
    14821 atcgtcgtat gggagtcaaa atcatcaggt ctcctggtcg gatttaatca ttccaacacc
    14881 ttatattttt cacaaatttg agagttgaat ctcaaatcat atcaaaaata gctgtcaaga
    14941 gcaccccaag gaatagtcca aatctgaaac tatgtcacgt gttaacgatt cagattggcg
    15001 ctaaatcgca gaaaatgtgg gggttatcgc aaaattcagc cgttttttgc gcgagatcgc
    15061 tcacccttgc ttctcatcct gtggacttac cgctcaggga tgagttttgt ctggcttatc
    15121 gctggcaaac tgtctgaaat cgcagcaata aggactcatc caccatgaaa ctcgccgcct
    15181 gttttctgac actccttcct ggcttcgccg ttgccgccag ctggacttct ccggggttcc
    15241 ctgcctttag cgaacaggga acgggaacat ttgtcagcca cgcgcagttg cccaaaggta
    15301 cgcgtccact cacgctaaat tttgaccaac agtgctggca gcctgcagat gcgataaaac
    15361 tcaatcagat gctttccctg caaccttgta gcaacacgcc gcctcaatgg cgattgttca
    15421 gagacggcaa atatacgctg caaatagaca cccgctccgg tacgccaaca ttgatgattt
    15481 ccatccagaa cgccgccgaa ccggtagcaa acctggtccg tgaatgcccg aaatgggatg
    15541 gattaccgct cacgctggat gtcagcgcca ctttcccgga aggagccgcc gtacgggatt
    15601 attacagcca gcaaattgcg atagtgaaga acggtcaaat aacgttacaa cccgctgcta
    15661 ccagcaacgg tttactcctg ctggaacggg cagaaactga cgcccctgcc cctttcgact
    15721 ggcataacgc cacggtttac tttgtgctga cagatcgttt cgaaaacggc gatcccagta
    15781 atgaccagag ttacggacgt cataaagacg gtatggcgga aattggcact tttcacggcg
    15841 gcgatttacg cggcctgacc aacaaactgg attacctcca gcagttgggc gttaatgctt
    15901 tatggataag cgccccattt gagcaaattc acggctgggt cggcggcggt acaaaaggcg
    15961 atttcccgca ttatgcctac cacggttatt acacacagga ctggacgaat cttgatgcca
    16021 atatgggcaa cgaagccgat ctacggacgc tggttgatag cgcacatcag cgcggtattc
    16081 gtattctctt tgatgtcgtg atgaaccaca ccggctatgc cacgctggcg gatatgcagg
    16141 agtatcagtt tggcgcgtta tatctttctg gtgacgaggt gaaaaaaacg ctgggtgaac
    16201 gctggagcga ctggaaacct gccgccgggc aaacctggca tagctttaac gattacatta
    16261 atttcagcga caaaacaggc tgggataaat ggtggggaaa aaactggatc agaacggata
    16321 tcggcgatta cgacaatcct ggattcgacg atctcactat gtcgctggcc tttttgccgg
    16381 atatcaaaac cgaatcaact accgcttctg gtctgccggt gttctataaa aacaaaacgg
    16441 atactcacgc taaagtcatc gaaggcttta cacctcgcga ttacttaacc cactggttaa
    16501 gtcagtgggt ccgcgactat gggattgatg gttttcgggt cgataccgcc aaacatgttg
    16561 agttgcccgc ctggcagcaa ctgaaaaccg aagccagcgc cgcgcttcgc gaatggaaaa
    16621 aagctaaccc cgacaaagca ttagatgaca aacctttctg gatgaccggt gaagcctggg
    16681 gccacggcgt gatgcaaagt gactactatc gccacggctt cgatgcgatg atcaatttcg
    16741 attatcagga gcaggcggcg aaagcagtcg attgtctggc gcagatggat acgacctggc
    16801 agcaaatggc ggagaaattg cagggtttca acgtgttgag ctacctctcg tcgcatgata
    16861 cccgtctgtt ccgtgaaggg ggcgacaaag cagcagagtt attactatta gcgccaggcg
    16921 cggtacaaat cttttatggc gatgaatcct cgcgtccgtt cggtcctaca ggttctgatc
    16981 cgctgcaagg tacacgttcg gatatgaact ggcaggatgt tagcggtaaa tctgccgcca
    17041 acgtcgcgca ctggcagaaa atcagccagt tccgcgcccg ccatcccgca attggcgcgg
    17101 gcaaacaaac gacactttcg ctgaagcagg gctacggctt tgttcgtgag catggcgacg
    17161 ataaagtgct ggtcatctgg gctgggcaac agtgactttt ccggcttccc gttcgtcagt
    17221 acctcgggaa gccgccaacc aggataaaat gtcagcccta atcagcgttg caggataaag
    17281 caccgctcac tctccaacag accgatttgc accccagcaa atgtagcgtt attgttacct
    17341 tccttgctac agagttcgac agatatcccg ctatgacatt ctcccttttt ggtgacaaat
    17401 ttacccgcca ctccggcatt acgctgctga tggaagatct gaacgacggt ttacgcacgc
    17461 ctggcgcgat tatgctcggt ggcggtaatc cggcgcagat cccggaaatg caggactact
    17521 tccagacgct actgaccgac atgctggaaa gtggcaaagc gactgatgca ctgtgcaatt
    17581 acgacggtcc acaggggaaa acggagctac tcacactgct tgccggaatg ctgcgcgaga
    17641 agttgggttg ggatatcgaa gcacagaata ttgcactaac aaacggcagc cagagcgcgt
    17701 ttttctactt atttaatctg tttgccggac gccgtgccga tggtcgggtc aaaaaagtgc
    17761 tgttcccgct ggcaccggaa tacattggct acgctgacgc cggactggaa gaagatctgt
    17821 ttgtctctgc gcgtccgaat attgaactgc tgccggaagg ccagtttaaa taccacgtcg
    17881 attttgagca tctgcatatt ggcgaagaaa ccgggatgat ttgcgtctcc cggccgacga
    17941 atccaacagg caatgtgatt actgacgaag agttgctgaa gcttgacgcg ctggcgaatc
    18001 aacacggcat tccgctggtg attgataacg cttatggcgt cccgttcccg ggtatcatct
    18061 tcagtgaagc gcgcccgcta tggaatccga atatcgtgct gtgcatgagt ctttccaagc
    18121 tggggctgcc tggctcccgc tgcggcatta tcatcgccaa tgaaaaaatc atcactgcca
    18181 tcaccaatat gaacggcatt atcagcctgg cacctggcgg tattggtccg gcgatgatgt
    18241 gtgaaatgat taagcgtaac gatctgctgc gcctgtctga aacagtcatc aaaccgtttt
    18301 actaccagcg tgttcaggaa actatcgcca tcattcgccg ctatttaccg gaagatcgct
    18361 gcctaattca taaaccagaa ggagccattt tcctctggct ttggtttaag gatttgccca
    18421 ttacgaccga gcagctctat cagcgcctga aagcacgcgg cgtgctgatg gtgccggggc
    18481 acaacttctt cccagggctg gacaaaccgt ggccgcatac gcatcaatgt atgcgcatga
    18541 actacgtacc agagccggag aaaattgagg cgggggtgaa gattctggcg gaagagatag
    18601 aaagagcctg ggctgaaagt cactaaaaat tgcctgatgc gctacgctta ttaggcctac
    18661 atgatttctg caatatattg aatttgcaag aatttgtggg ccggataagg cgtttacgcc
    18721 gcatctggca taaacaaagc gcactattcc ggcgttgaga aacgccggaa aaacgtttca
    18781 aacaggctgc tgccgtagcc gatgccgctg cactttctcg acatccatgc actgcaacgc
    18841 ctttgtcggg caggcttcaa cacaggccgg ccccgtctcc cgatgccagc acagatcgca
    18901 cttaatcgcc ctcgcctttt tgcgcgaaga aacgacctcc atcgcaccaa acgggcaagc
    18961 cagcatacag cttttacagc caatgcaacg tgtttgttca acgaaaatat gcccatgttc
    19021 gcggcttatc gcgttaacag ggcagacatt cgcgcacggt gcatcttcac actgatgaca
    19081 ggctactgcc gtggtccagc tgtggtcttt aatgacacga atacgggaaa taaactcgtc
    19141 tggtgacaac gcagcgcaat cctgattctc ctgatgcgac actgcgcaag ccacttcaca
    19201 ggtacggcaa ccgatacatt tcgtcgcatc cgcaataata aaccggttca tgatcactcc
    19261 tttgcactaa aacagcagag tgccagagag cgcgtcccta ctgctttgat ccaacgaagg
    19321 aaatgttaaa gttttggcac ttgtccaaag cgttatgtga tcgcgccctg gtcgtccctg
    19381 acagtaaatc ccagttcatt agaaatagcc tgcgcggttt cacgcagtgg tttcaggaga
    19441 tttttctccc ccacctgttt cagacgtgat gtcgaaagcg aaatcgacac ggcgtacggc
    19501 acccgcccat gaatattaaa caccggaaca gcaatacagg agacgccgag ttcgttttct
    19561 tctctgtcca tcgccgctcc gctttcacga atgtgcgcca gttcgtcgaa catcgcgggc
    19621 agctcggtaa tggtattgcg ggttaacggc tggatctcat gctgatggtt ttcccagtat
    19681 gacttcacgt agtccgggtg accaaacgcc atgtagatct tgcccattgc ggaacagtag
    19741 agcggcatat gctggccaat ataggcacgg gttcgcagca tcccggttgt gggttccagc
    19801 ttataaatca aaatagcgtg atcgtcttcg cggctggaga agttaatggt ttcaccagtg
    19861 gcgatgttca gtgcctcaag atgcggagcg gcgatatgaa tgatattcag cgaagacagc
    19921 gccttctgcc cgacggcaat aaatttggta gtcaggcggt aactccctgc ggcgggcgcg
    19981 gtggtcacat agccgcagga ctgtaatccc tgcaataagc gatggacggt actcttattt
    20041 aaaccagcca gctccgaaag atgcgccaac ggacaaccgt ttggatagtt gctcaaaatc
    20101 tcaatcagca tcaacccgcg aaacagactc tggcttccgg ctggacgctc tttttcctgc
    20161 gccatctcgt tctctttttt tcccatcact tctttcccca ttttgtcgcg tcctgatggt
    20221 agcgcaaagt gtgccgtagt tcacgatctc gacagataat ttataaccac ttgattttta
    20281 tgtcttttga aattcatcaa tcagattgct ttgttaaaaa gcgatcgata tatttgaaat
    20341 caagtttcgc atattgaaat tttaagccaa aaaagcgatc aaaaaaacaa ggaagcctgg
    20401 gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa tttcacgcgg
    20461 cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca ccgaatccgg
    20521 cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg aaaacggcga
    20581 tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg aacagtggga
    20641 tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg ccattgaact
    20701 ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact ggatgcgcgg
    20761 cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct ggaccaactc
    20821 catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc attggcacta acccgctgat
    20881 cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga tgttctctta
    20941 cggcatgtta gaagttaacc gcctggcggg ccgtcagctc ccggtcgatg gtggctttga
    21001 tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc gcattttgcc
    21061 gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg ctactctcct
    21121 ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat acggcatttc
    21181 acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc gcgatgccaa
    21241 gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa atcaggccat
    21301 ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta acggtattac
    21361 cgttgatgac agcgtgtggg ccaaaatcca ggcgttataa ggagataagt catgattttt
    21421 ggacatatcg cgcagcccaa tccgtgccgt ttgcccgccg ccattgaaaa ggcgctcgat
    21481 tttctgcgcg ctaccgattt caacgccctg gagccgggcg ttgtcgaaat cgacggcaaa
    21541 aatatttatg cgcaaattat cgatttaacc acacgcgaag cggtggaaaa ccgtccggaa
    21601 gtgcatcgtc ggtatatcga tattcagttt ctggcgtggg gtgaagagaa aatcggcatt
    21661 gctattgata cgggaaataa taaagtcagc gaatcactat tagagcagcg cgatattatt
    21721 ttttatcatg acagtgaaca tgaatcattt atagaaatga taccgggcag ctacgcaatt
    21781 ttctttccgc aggatgttca ccgaccaggt tgtattttgc aaacagcctc tgagatacga
    21841 aaaattgtgg ttaaagtcgc attaacggcg ctgaattaat cgaataatcg tctacgccag
    21901 aacgactggg taatgttatt gctctaacta tattaataat ttaggttgct gcacatgcaa
    21961 cttgaattat gacagacata gcctcaagga atagctatga aaaaaatact tgaagcaata
    22021 ctggcgatta atctcgccgt actttcctgt attgtgttta taaacattat tttaagatat
    22081 ggttttcaga caagcatttt atctgtagat gaattgtcac gttatttatt tgtctggtta
    22141 acatttattg gcgcgattgt ggcttttatg gataacgccc acgttcaggt cacctttctg
    22201 gtggagaaac tctcccccgc atggcaacga cgagtcgctc tggttaccca ttccttaatc
    22261 ttatttattt gtggcgcact ggcctggggc gcaacgctaa aaacaattca ggactggagc
    22321 gattattcac cgatcctcgg tttacccatc ggcctgatgt atgccgcttg tctgcccacc
    22381 agcctcgtta tcgcattctt tgaactgcgt catttatatc aactcatcac gcgtagcaat
    22441 tctctaacgt cgccaccgca aggagcttaa tcatggctgt gctgattttt ctgggctgtc
    22501 tgttgggtgg tattgctatc ggtttgccta ttgcctgggc actgttgttg tgcggggcgg
    22561 cgttaatgtt ctggctggac atgtttgatg tccagatcat ggcacaaacg ctggtgaacg
    22621 gagccgatag cttctccctg ctggcgattc cgttctttgt gctggcgggt gaaatcatga
    22681 atgcgggcgg cttgtcaaaa cgcattgttg acctgccgat gaaactggtg gggcataaac
    22741 caggcgggct gggctacgtc ggcgtgctgg cagctatgat tatggcgagc ctttctggtt
    22801 ctgccgttgc ggataccgcc gccgttgccg ctctactggt gccgatgatg cgcagcgcca
    22861 actacccggt caaccgggcg gcggggctga ttgcctctgg cggcattatc gcgccaatta
    22921 ttcccccttc cattccgttt attatcttcg gcgtttccag cggattatcc atcagtaagc
    22981 tgtttatggc gggcattgcc cctggcatga tgatgggcgc aacgctgatg cttacctggt
    23041 ggtggcaggc cagtcgcctt aatttacccc gccagcaaaa agcaacgatg caggaaatct
    23101 ggcactcttt tgtctccggt atctgggcgc tgtttcttcc agtcatcatt attggcgggt
    23161 ttcgctccgg tctgtttacc cctaccgaag ccggggccgt tgctgctttc tatgcgctgt
    23221 ttgtcgccac agttatttac cgtgaaatga ccttcgccac actttggcat gtactcattg
    23281 gcgcggcgaa aaccacctca gtggttatgt ttctggtggc ctcggcacaa gtttccgcct
    23341 ggctgattac cattgctgaa ctgccgatga tggtttctga tttactgcaa ccgttggtcg
    23401 attcaccgcg tctgctgttt atcgtcatta tggtggctat cctgattgtc ggcatggtca
    23461 tggatttaac gccgacggta ttaattctta ccccggtatt aatgccttta gttaaagagg
    23521 caggaatcga tccgatttat ttcggtgtca tgtttatcat taactgctca atcggcttaa
    23581 ttacaccgcc tatcggtaat gtacttaacg ttatttcagg ggtggcaaaa ctcaaattcg
    23641 a
//
LOCUS       DAZFXT010000072        23504 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.072, whole genome shotgun sequence.
ACCESSION   DAZFXT010000072 DAZFXT010000000
VERSION     DAZFXT010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23504
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.072"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(183..938)
                     /gene="mlaA"
                     /locus_tag="ACHKW0_003015"
     CDS             complement(183..938)
                     /gene="mlaA"
                     /locus_tag="ACHKW0_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HFO7475814.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            1232..2164
                     /locus_tag="ACHKW0_003016"
     CDS             1232..2164
                     /locus_tag="ACHKW0_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HFO7475815.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            2240..2314
                     /locus_tag="ACHKW0_003017"
     tRNA            2240..2314
                     /locus_tag="ACHKW0_003017"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2273..2275,aa:Arg,seq:cct)
     gene            complement(2756..4003)
                     /locus_tag="ACHKW0_003018"
     CDS             complement(2756..4003)
                     /locus_tag="ACHKW0_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HFO7475816.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(4075..4989)
                     /locus_tag="ACHKW0_003019"
     CDS             complement(4075..4989)
                     /locus_tag="ACHKW0_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HFO7475817.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            5205..6638
                     /gene="cscA"
                     /locus_tag="ACHKW0_003020"
     CDS             5205..6638
                     /gene="cscA"
                     /locus_tag="ACHKW0_003020"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HFO7475818.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(6646..7641)
                     /locus_tag="ACHKW0_003021"
     CDS             complement(6646..7641)
                     /locus_tag="ACHKW0_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005088955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7475819.1"
                     /translation="MASLKDVARLAGVSMMTVSRVMHNAESVRPATRDRVLQAIQTLN
                     YVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDILLAIEQTASEFGWNSFLINIFS
                     EDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYGENIVLANCVADDPALPSYIPD
                     DYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQGFEQAWRDAGRDLAEVKQFHM
                     ATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAFVAYQVLLAKGVRIPQDVAVMG
                     FDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGREGGRVTRIPCPLLIRCST"
     gene            7884..8102
                     /locus_tag="ACHKW0_003022"
                     /pseudo
     CDS             7884..8102
                     /locus_tag="ACHKW0_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            8120..9448
                     /gene="dsdA"
                     /locus_tag="ACHKW0_003023"
     CDS             8120..9448
                     /gene="dsdA"
                     /locus_tag="ACHKW0_003023"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008721 - D-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0046416 - D-amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HFO7475820.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(9556..11094)
                     /gene="emrY"
                     /locus_tag="ACHKW0_003024"
     CDS             complement(9556..11094)
                     /gene="emrY"
                     /locus_tag="ACHKW0_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HFO7475821.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(11094..12257)
                     /gene="emrK"
                     /locus_tag="ACHKW0_003025"
     CDS             complement(11094..12257)
                     /gene="emrK"
                     /locus_tag="ACHKW0_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HFO7475822.1"
                     /translation="MEQINSNKKHSNRRKYFSLLAVVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKETWLALKRTDIRSPVTGY
                     IAQRSVQVGETVSPGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKNEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            12673..13287
                     /gene="evgA"
                     /locus_tag="ACHKW0_003026"
     CDS             12673..13287
                     /gene="evgA"
                     /locus_tag="ACHKW0_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HFO7475823.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            13292..16885
                     /gene="evgS"
                     /locus_tag="ACHKW0_003027"
     CDS             13292..16885
                     /gene="evgS"
                     /locus_tag="ACHKW0_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0010447 - response to acidic pH [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HFO7475824.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNSDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(16941..18086)
                     /gene="yfdE"
                     /locus_tag="ACHKW0_003028"
     CDS             complement(16941..18086)
                     /gene="yfdE"
                     /locus_tag="ACHKW0_003028"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HFO7475825.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWETLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(18160..19104)
                     /gene="yfdV"
                     /locus_tag="ACHKW0_003029"
     CDS             complement(18160..19104)
                     /gene="yfdV"
                     /locus_tag="ACHKW0_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HFO7475826.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(19174..20868)
                     /gene="oxc"
                     /locus_tag="ACHKW0_003030"
     CDS             complement(19174..20868)
                     /gene="oxc"
                     /locus_tag="ACHKW0_003030"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008949 - oxalyl-CoA decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HFO7475827.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(20922..22172)
                     /gene="frc"
                     /locus_tag="ACHKW0_003031"
     CDS             complement(20922..22172)
                     /gene="frc"
                     /locus_tag="ACHKW0_003031"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033608 - formyl-CoA transferase activity
                     [Evidence IEA];
                     GO_process: GO:0033611 - oxalate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HFO7475828.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(22685..23317)
                     /locus_tag="ACHKW0_003032"
     CDS             complement(22685..23317)
                     /locus_tag="ACHKW0_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HFO7475829.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         6733 a         5488 c         5106 g         6177 t
ORIGIN      
        1 aaattatttt tccagttttt tgatttttca actcctgaat atcggaaata gtcagtgacg
       61 aacatcagta tttttacatc attacgtccg ggtcattgtc ggtttatctc cttttacttg
      121 tggtaattga gggataacaa caaaaaaggt gagtattgcg actcaccttt tttatttgtt
      181 tcttattcag aatcgatatc ttttaaatca tcctgaatcg cttgcgcgtt cggattttcc
      241 tgcggtttga gttcgccgcc attagcgatg aaatcatgac gctggaagta cgcttcgcgc
      301 accataatat aaggatcgga cgactgacgc agcagaccat cggaatccaa caactgcgca
      361 cgagtttcta tcccttcaag cgtccattta cccacagaca tcggccaggt cagccaggaa
      421 agaaccgggt aaagaccatc cgccatatca ccaccgtcat cacgcagcgt gaagctaccg
      481 tagaacggta actggacgta aggcccataa cccacgccat aatgaccaag cgtactaccg
      541 aagcggtgag gttcagtccg ttgcagtttc gggttcgcca tccctgcaac atcaataaaa
      601 ccgcccatcc ccaaaatggt gttcaggaaa aagcgggtaa agtggaccat cccctgataa
      661 gggtcgccct gcaagaagta gttaaccatc accgcaggtt cttcaaggtt gccagtaaag
      721 ttgctcaaac cgttacgcgc cggttgcgga acataatcac gccaggcgac agcgaccggt
      781 cgaacaatat acgggtctaa tacattgaag ttgaagttgt acatggtgcg gttgaaccct
      841 tctaacgggt cagaacgccc ttgctgatct gtaccggaac tcgcacaccc caccagaagc
      901 gtagttccca gagcaagcgc cgacaggcga agcttcataa atgtctccct gtttttttat
      961 ggcttatgca gtttgccatc catgacggaa cgataccgta tccgcctgtt taggtgtggg
     1021 cgattgtaac agcacgtcaa ctgatgtcca gacgccctga tttgctgatt tgatcatagc
     1081 ctggtaatcg ccgccctgta ggctacttga ttctatagaa acagaaaaag gcaaacgcag
     1141 ccttttctac attttcagag taactcccgc cagttgcgag caaaaaagcc gctacgcttt
     1201 agctatacgt gctaattcaa gagaagagac catggacaac gacaaaattg atcaacacag
     1261 cgacgaaatt gaagttgaga gcgaagaaaa agagcgcggc aaaaaaatag aaatagatga
     1321 agaccgactc ccctcccggg cgatggcaat tcatgagcat atccgccagg atggtgaaaa
     1381 agagctggaa cgcgacgcaa tggcgctact gtggtcagcc attgcggcgg gtctgtcgat
     1441 gggcgcttcg ctactggcaa aagggatatt tcatgtcgaa ctggaaggag tgccaggcag
     1501 cttcttactg gagaatctcg gttatacctt tggttttatt atcgtcatta tggcccgcca
     1561 gcaattattt accgaaaaca ccgtgactgc ggtactaccc gtcatgcaaa aaccgacaat
     1621 gagcaacgtc ggcttactta tgcgattatg gggcgtcgtg ctgctgggta atattctcgg
     1681 gacaggtatt gctgcatggg catttgaata tatgcctatc ttcaatgaag aaactcgcga
     1741 tgcatttgtc aaaatcggca tggatgtgat gaagaacacc cccagcgaga tgtttgccaa
     1801 cgcgatcatt tccggctggc tgatcgccac tatggtttgg atgtttcctg ctgcgggtgc
     1861 ggcaaagatt gtggtgatta tattgatgac ctggcttatt gcactgggtg acaccaccca
     1921 catcgttgtc ggttctgttg aaatcctcta tctggtgttt aacggcacgc tgcactggag
     1981 cgatttcatc tggcccttcg cactacctac tttagcgggg aacatctgcg gcggcacctt
     2041 tatcttcgcg ttaatgagtc atgcacagat tcgtaacgac atgagcaaca agcgtaaagc
     2101 agaagcacgc caaaaagcag aacgtgcgga aaacattaag aaaaatgata aaaacccagc
     2161 ataaatggcg agggtttaag caatcgagcg gcagcgtact taccccgcag tccattagcg
     2221 ggtatactca tgccgcattg tcctcttagt taaatggata taacgagccc ctcctaaggg
     2281 ctaattgcag gttcgattcc tgcaggggac accagatacc cttcaaacga aatctacctt
     2341 caccccgtaa aagatgggtt tggcagcaca cttgccttat atctactcat ttttactgca
     2401 acaggttgaa atctcagcac tgtcagaaag cgctgatgac taaacagccc tgggccgggc
     2461 gatgtaacca tcacacagaa tcctgatagc gaaatatggc gtgactcgat acttcactcc
     2521 gcaatgcatt ccttgatgaa ttcgcaggac cgtgatacac gggacaggtc actgaatgac
     2581 gacaatgtcc tggaaatcag cgaaccgcgc atctgaagta catttgagcg actgtaccag
     2641 aacatgaatg aggcgtttgg attaggcgat tattagcagg gctaagcatt ttactattat
     2701 tattttccgg ttgagggata tagagctatc gacaacaacc ggaaaaagtt tacgtctata
     2761 ttgctgaagg tacaggcgtt tccataacta tttgctcgcg ttttttactc aagaagaaaa
     2821 tgccaaatag caacatcagg cagacaatac ccgaaattgc gaagaaaact gtctggtagc
     2881 ctgcgtggtc aaagagtatc ccagtcggcg ttgaaagcag cacaatccca agcgaactgg
     2941 caatttgaaa accaatcaga aagatcgtcg acgacaggcg cttatcaaag tttgccacgc
     3001 tgtatttgaa gacggatatg acacaaagtg gaacctcaat ggcatgtaac aacttcacta
     3061 atgaaataat ccaggggtta acgaacagcg cgcaggaaag gatacgcaac gccataatca
     3121 caactccgat aagtaatgca ttttttggcc ctacccgatt cacaaagaaa ggaataatcg
     3181 ccatgcacag cgcttcgagt accacctgga atgagttgag ataaccatac aggcgcgttc
     3241 ctacatcgtg tgattcgaat aaacctgcat aaaagacagg aaaaagttgt tgatcaaaaa
     3301 tgttatagaa agaccacgtc cccacaataa atatgacgaa aacccagaag tttcgatcct
     3361 tgaaaactgc gataaaatcc tcttttttta cccctcccgc atctgccgct acgcactggt
     3421 gatccttatc tttaaaacgc atgttgatca tcataaatac agcgccaaat agcgagacca
     3481 accagaagtt gatatgggga ctgatactaa aaaatatgcc ggcaaagaac gcgccaatag
     3541 catagccaaa agatccccag gcgcgcgctg ttccatattc gaaatgaaaa tttcgcgcca
     3601 ttttttcggt gaagctatca agcaaaccgc atcccgccag ataccccaag ccaaaaaata
     3661 gcgcccccag aattagacct acagaaaaat tgctttgcag taacggttca taaacgtaaa
     3721 tcataaacgg tccggtcaag accaggatga aactcataca ccagatgagc ggtttcttca
     3781 gaccgagttt atcctgaacg atgccgtaga acatcataaa tagaatgctg gtaaactggt
     3841 tgaccgaata aagtgtacct aattccgtcc ctgtcaaccc tagatgtcct ttcagccaaa
     3901 tagcgtataa cgaccaccac agcgaccagg aaataaaaaa gagaaatgag taactggatg
     3961 caaaacgata gtacgcattt ctgaatggaa tattcagtgc cataattacc tgcctgtcgt
     4021 taaaaaattc acgtcctatt tagagataag agcgacttcg ccgtttactt ctcactattc
     4081 cagttcttgt cgacatggca gcgctgtcat tgcccctttc gccgttactg caagcgctcc
     4141 gcaacgttga gcgagatcga taattcgtcg catttctctc tcatctgtag ataatcccgt
     4201 agaggacaga cctgtgagta acccggcaac gaacgcatct cccgcccccg tgctatcgac
     4261 acaattcaca gacattccag caaaatggtg aacttgtcct cgataacaga ccaccacacc
     4321 ttctgcacct ttagtcacca acagcatggc gatctcatac tcttttgcca gggcgcatat
     4381 atcccgatcg ttctgtgttt ttccactgat aagtcgccat tcttcttccg agagcttgac
     4441 gacatccgcc agttgtagcg cctgccgcaa acacaagcgg agcaaatgct cgtcttgcca
     4501 tagatcttca cgaatattag gatcgaagct gacaaaacct ccggcatgcc ggatcgccgt
     4561 catcgcagta aatgcgctgg tacgcgaagg ctcggcagac aacgcaattg aacagagatg
     4621 taaccattcg ccatgtcgcc agcagggcaa gtctgtcgtc tctaaaaaaa gatcggcact
     4681 ggggcggacc ataaacgtaa atgaacgttc cccttgatcg ttcagatcga caagcaccgt
     4741 ggatgtccgg tgccattcat cttgcttcag atacgtgata tcgactccct cagttagcag
     4801 cgttctttgc attaacgcac caaaaggatc atcccccacc cgacctataa acccacttgt
     4861 tccgcctaat ctggcgattc ccaccgcaac gttagctggc gcgccgccag gacaaggcag
     4921 taggcgcccg tctgattctg gcaagagatc tacgaccgca tcccctaaaa cccatacttt
     4981 ggctgacatt tttttccctt aaattcatct gagttacgca tagtgataaa cctctttttc
     5041 gcaaaatcgt catggattta ctaaaacatg catattcgat cacaaaacgt catagttaac
     5101 gttaacattt gtgatattca tcgcatttat gaaagtaagg gactttattt ttataaaagt
     5161 taacgttaac aattcaccaa atttgcttaa ccaggatgat taaaatgacg caatctcgat
     5221 tgcatgcggc gcaaaacgcc ctagcaaaac ttcatgagca ccggggtaac actttctatc
     5281 cccattttca cctcgcgcct cctgccgggt ggatgaacga tccaaacggc ctgatctggt
     5341 ttaacgatcg ttatcacgcg ttttatcaac atcatccgat gagcgaacac tgggggccaa
     5401 tgcactgggg acatgccacc agcgacgata tgatccactg gcagcatgag cctattgcgc
     5461 tagcgccagg agacgataat gacaaagacg ggtgtttttc aggtagtgct gtcgatgaca
     5521 atggtgtcct ctcacttatc tacaccggac acgtctggct cgatggtgca ggtaatgacg
     5581 atgcaattcg cgaagtacaa tgtctggcta ccagtcggga tggtattcat ttcgagaaac
     5641 agggtgtgat cctcactcca ccagaaggaa tcatgcactt ccgcgatcct aaagtgtggc
     5701 gtgaagccga cacatggtgg atggtagtcg gggcgaaaga tccaggcaac acggggcaga
     5761 tcctgcttta tcgcggcagt tcattgcgtg aatggacctt cgatcgcgta ctggcccacg
     5821 ctgatgcggg tgaaagctat atgtgggaat gtccggactt tttcagcctt ggcgatcagc
     5881 attatctgat gttttccccg cagggaatga atgccgaggg atacagttac cgaaatcgct
     5941 ttcaaagtgg cgtaataccc ggaatgtggt cgccaggacg actttttgca caatccgggc
     6001 attttactga acttgataac gggcatgact tttatgcacc acaaagcttt ttagcgaagg
     6061 atggtcggcg tattgttatc ggctggatgg atatgtggga atcgccaatg ccctcaaaac
     6121 gtgaaggatg ggcaggctgc atgacgctgg cgcgcgagct atcagagagc aatggcaaac
     6181 ttctacaacg cccggttcac gaagctgagt cgttacgcca gcagcatcaa tctgtctctc
     6241 cccgcacaat cagcaataaa tatgttttgc aggaaaacgc gcaagcagtt gagattcagt
     6301 tgcagtgggc gctgaagaac agtgatgccg aacattacgg attacagctc ggcactggaa
     6361 tgcggctgta tattgataac caatctgagc gacttgtttt gtggcggtat tacccacacg
     6421 agaatttaga cggctaccgt agtattcccc tcccgcagcg tgacacgctc gccctaagga
     6481 tatttatcga tacatcatcc gtggaagtat ttattaacga cggggaagcg gtgatgagta
     6541 gtcgaatcta tccgcagcca gaagaacggg aactgtcgct ttatgcctcc cacggagtgg
     6601 ctgtgctgca acatggagca ctctggctac tgggttaaca taatatcagg tggaacaacg
     6661 gatcaacagc gggcaaggga tccgcgtcac tcttccccct tcacgacctt caataatatg
     6721 caatgcagct tcccgcccga taatgtcatg tggaagctga attgtggtca gcggcggtaa
     6781 aaacagatgc ccgacgccaa ccagattatc aaagcccatt acggcgacat cctgcgggat
     6841 acgtaccccc ttcgccagga gaacctgata agcgacaaag gctgcgcgat cgttaccaca
     6901 tatcagaaca tcaaaatctg gtttgcccgg tttgaagtgg gcattgagta aacttgcgag
     6961 atcggtgtag tgatcatcac ctgttgccat gtgaaattgt ttcacctcag ccagatctcg
     7021 tccagcatca cgccaggcct gctcaaatcc ctgccgacga taccctgttg ccaacgcact
     7081 ttccggtagc cagaagcata acggttgacg atagcccgcc gcgagcaaat gctgtgttga
     7141 ttcatattgt gcagtgtaat catcagggat ataactgggt aacgctgggt catccgccac
     7201 acagttcgcc aatacaatat tttcaccata cagagactca ggcagcgtga tatgtcgcag
     7261 ccccattgta gtatagataa tgccatccgg acggtgggca agcagctgac gtgccgcgcg
     7321 ggcagcgtca tcttcagaaa aaatattgat taaaaaacta ttccagccga actcgctggc
     7381 ggtttgctca atggcaagca gaatatcaac agagaaagga gtggtagccg tgtcctgcgc
     7441 cagcacggcg agagtcgacg gcttacgtcc ttgagcgcgc atcttacggg cggaaagatc
     7501 aggaacataa ttcagggtct ggattgcctg caatacgcgg tcacgcgttg caggacgcac
     7561 agattctgca ttatgcatca cccgggagac tgtcatcatc gacactcccg ccaggcgtgc
     7621 gacatccttt aatgaagcca tacccaagcc gtttgccgta aaacgggcac tgtagcagaa
     7681 acagacgtca ctggcgagat ccaacgatat tctcaacgcc ctatcaccca acacggcaat
     7741 acaataaaaa ataacaataa ttgccggata attgtactca attccgcctc tgttctcgcc
     7801 aacgagtaac catataggca agtaagtaaa acatgccaac tggcatcgcc aacctggaga
     7861 agaaaatata aatatggatc attgtgctgc cgctgtatcc cgacatcagc ccggaaatta
     7921 ttgcgattgc tatcggttca ggtgcaattg gctgcacgat cgttacggac tcgcttttct
     7981 ggctagtgaa gcaatattgc ggcgctacgc tcaatgaaac atttaaatac tatacgacag
     8041 cgacatttat cgcttcagtc gtcgctctgg cgggcacatt cctgctgtca tttatcatct
     8101 aagcgcaaag agacgtacta tggaaaacgc taaaatgaac tcgctcatcg cccagtatcc
     8161 gttggtaaag gatctggttg ctcttaaaga aaccacctgg tttaatcctg gcacgacctc
     8221 attggctgaa ggtttacctt atgttggcct gaccgaacag gatgttcagg acgcccatgc
     8281 gcgcttatcc cgttttgcac cctatctggc aaaagcattt cctgaaactg ctgccactgg
     8341 ggggattatt gaatcagaac tggttgccat tccagctatg caaaaacggc tggaaaaaga
     8401 atatcagcaa ccgatcagcg ggcaactgtt actgaaaaaa gatagccatt tgcccatttc
     8461 cggctccata aaagcacgcg gcgggattta tgaagtcctg gcacacgcag aaaaactggc
     8521 tctggaagcg gggttgctga cgcttgatga tgactacagc aaactgcttt ctccggagtt
     8581 taaacagttc tttagccaat acagcattgc tgtgggctca accggaaatc tggggttatc
     8641 aatcggcatt atgagcgccc gcattggctt taaggtgaca gttcatatgt ctgctgatgc
     8701 ccgggcatgg aaaaaagcga aactgcgcag ccatggcgtt acggtcgtgg aatatgagca
     8761 agattatggt gttgccgtcg aggaaggacg taaagcagcg cagtctgacc cgaactgttt
     8821 ctttattgat gacgaaaatt cccgcacgtt gttccttggg tattccgtcg ctggccagcg
     8881 tcttaaagcg caatttgccc agcaaggccg tatcgtcgat gctgataacc ctctgtttgt
     8941 ctatctgccg tgtggtgttg gcggtggtcc tggtggcgtc gcattcgggc ttaaactggc
     9001 gtttggcgat catgttcact gcttttttgc cgaaccaacg cactcccctt gtatgttgtt
     9061 aggcgtccat acaggattac acgatcagat ttctgttcag gatattggta tcgacaacct
     9121 taccgcagcg gatggccttg cagttggtcg cgcatcaggc tttgtcgggc gggcaatgga
     9181 gcgtctgctg gatggcttct atacccttag cgatcaaacc atgtatgaca tgcttggctg
     9241 gctggcgcag gaagaaggta ttcgtcttga accttcagca ctggcgggta tggccggacc
     9301 tcagcgcgtg tgtgcatcag taagttacca acagatgcac ggtttcagcg cagaacaatt
     9361 gcgtaatgcc actcatctgg tgtgggcgac gggaggtgga atggtgccgg aagaagagat
     9421 gaatcaatat ctggcaaaag gccgttaata acgtttcaac gcagcatcgc aatcctttcc
     9481 ctgggtgagc gatgctgccg atggcgcaga cttaagatcc ccggtcttac ccgctataac
     9541 cccctccttt tatcatcacc caacgccttt cgctgtaaac ggcggtttcg caaaccaaac
     9601 caaaaccgta agtaagataa aagcaatagc cgccatacgg aaaatctcat ttgcagaaat
     9661 agaaagtgac tgctgggtta tttcattatt aatttcatta agaactcctg aaagcgaacc
     9721 atagtattta tccataattt gtgacgatga attaaacacg gggttaaatt gatcgatggt
     9781 tgctgtcaac tgactatggt gtaacgattc gcgtcgtccc cacagcgtca ttgtcaacga
     9841 cgtaccaact gatcctgaca aggtacgaaa aaaattactc atactcgagg cattggcaaa
     9901 tttattatct ggcaagcctg aaaacgaaat cgttgttaag ggtaaaaaga aacaggcaac
     9961 ggcgaatccc tgaaaaaact gcggcaaaat gatgcctgta aaatcaatcg ttggcataaa
    10021 tgtcacagaa cgccagtaat agcaaaccgc atacatcaaa aaactaaatg tcactaacaa
    10081 ccgcatgtct attttgttgc cataacgtcc tatcaaaggt gaaattaata gtggcatgat
    10141 gccgatgggc gcataagcaa gtccggccca tatcgcatta taccccatcg tttcctggag
    10201 taactgcggc ataaggacga tcgctccaga gtaaaataaa tacgcgcatg tgatactcac
    10261 aataccaatg gtgaagttac gggacttaaa caaactgaga tcaagaatcg ggttctctga
    10321 ggtcgactcc caaatgacta aagagatcag agaaataact gatactactg ttaatattat
    10381 tattgtactc gagttgaacc aatccagatc gcgcccttta tcaagcataa tttgcaagcc
    10441 accaacaccg agcactaaca gggtcagtcc tggtagattc attttgaccg gtgaagtttc
    10501 agtttctctt cctttaagta aggttaagca taatgtcagg acgataatcc ccatagggac
    10561 attgattaaa aatatccaac cccagctaaa gttatcgcaa atataaccgc ccaatatcgg
    10621 cccacatatc ggagcgataa tcacggtcat tgaccataat gccagagcaa atgttctttt
    10681 ttctggtgga taattcctta ataataaact ctgtgacagt ggaattaacg gccccgccat
    10741 taatccctga acgactctaa aaaatatcag cacatcaaga ttggtcgata aactacacat
    10801 taaggaagac agcgaaaaaa aagtgactga aagtaaaaat aaccttaatt cgcctattct
    10861 ttgtgccagc ctgccggtaa cagggatcgc aatggcattt gctacgccaa acgaggtgat
    10921 aacccaggtg ccttcgtctg ttgatgctcc cagaaagcca gatattgtcg gtattgcgac
    10981 gttagaaata gtggaatcca acatttgcat aaacgtcgct aatgacaatg caatagtaac
    11041 gcaccataac gtcccaccgg ttaatggtgc cggagtcgat ttagtgattg ccattaaagt
    11101 tgtccattat gcgaaataat gttgctaatt tctttttcta tcggactggt atcgataact
    11161 aaagccttac tggtataagc cggcatggac gtcacggttg aagccagatc aggcatctca
    11221 gcaatgtctt cgttcttcgt atcaatagtt gcagtcatcg ataaaccaat gcgtaagggg
    11281 tgttccatga gttcttttgg atcaagagaa acttcaaccg gtacacgctg aacgattttg
    11341 atccagttcc ctgtcgcatt ttgtgcaggt aataaggaga acgcattgcc ggttcccata
    11401 ttgatccctg tcacccgacc atgaaacaca acattttcac cataaagatc gctgataata
    11461 ttgactgatt gaccaatccg tacatccgtg agttgtgttt ctttaaagtt ggcattaacc
    11521 cacatttgac gtgccggtac gacagccatt aacgattgtc cgggactcac tgtttcgccg
    11581 acctgaacac tccgctgggc aatatagcca gtaaccggac tccttatatc cgtacgttta
    11641 agcgccaacc aggtttcttt agttgcatcc gccgcttcca cgacttgtgg ctgacggttt
    11701 aatggtgtgt tcattactaa agctttattc gctttataag cctggatagc ggcattcagt
    11761 gccgctttgc tacttattaa cgtatctttg gtatgctcca gcgtttcttt tgaaataacc
    11821 ccctgcttcg ctaacggtac tcgacggtta taatcttcta aagattgttg atactgaata
    11881 cgtgctgaag cgacttcggc actgtattgt ttatcctgta agtacagttt attcgtttgc
    11941 cgaacaatat ttgccagatt atttttagct ttattgagtg cgatagtggc atcagtttta
    12001 tccagtgaaa ctaaaatgtc accttgtcga acgtagttcg tatctttatg attaacgaca
    12061 gtgacactac ctgagacttg tgcagaaatt ggatctgcat tccccgtgac ataggcgtca
    12121 tctgtactaa tcatgtcttc taattccatt gaccaatagg cataggcacc tgaaaacgca
    12181 ataaataaaa ctaccgccaa taaagaaaag tattttcttc tgttagaatg ttttttattt
    12241 gaattaatct gttccactat tatctctcat ttctcataga tgaaatttat gaattgaatg
    12301 aaaagagcat tcattcttca tatcaaacac agtgcatgtg ttgcacatta tcccaggaac
    12361 gttggaggcc taatttaaat gaggtgcaaa aaaaacaaca gaggactaaa ccgtggcttt
    12421 tgcaatacaa ttcttacgcc tgtaggatta gtgagaagac ttatagtgct aacttgaaac
    12481 tataaatcat cggtataatc cctgatttta ttgttgacat ttcatttatg ccgactattt
    12541 atatggtata cttgtcgaat tatcttaaag gaagctcaga ttttcttatt tttattgaga
    12601 aaatgagatg acgccttatg tctgtattac tacagggaga agggagatgc ttcattgcaa
    12661 agggaataat ctatgaacgc aataattatt gatgaccatc ctcttgctat cgcagcaatt
    12721 cgtaatttat tgatcaaaaa cgatattgaa atcttagcgg agttgactga agggggaagc
    12781 gccgttcagc gggtggaaac acttaagcct gatatcgtca tcattgatgt cgatatcccc
    12841 ggagttaacg gtatccaggt gttagaaacg ctgaggaagc gccaatatag cggaattatt
    12901 attattgtct ccgctaaaaa tgaccatttt tacgggaaac attgtgctga tgctggcgct
    12961 aatggattcg tgagtaaaaa agaaggcatg aacaatatca ttgcggctat tgaagctgca
    13021 aaaaatggct actgctattt ccccttctct ctcaaccggt ttgttggaag tttaacgtcc
    13081 gaccagcaaa aactcgactc cttatcgaaa caagaaatta gtgtcatgcg gtatattctt
    13141 gatggcaagg ataataatga cattgctgaa aaaatgttca tcagcaacaa aactgtcagc
    13201 acttataaaa gtcgcctgat ggaaaaatta gaatgtaaat cactgatgga tctttacaca
    13261 ttcgcgcaac gtaacaaaat cggctaatca catgaagttt ttaccctatt tttttcttct
    13321 ctgttgtggt ctttggtcga ccataagttt cgcagacgaa gattacatcg aatatcgtgg
    13381 catcagtagt aacaaccgtg tcacacttga tccagtacgt ctgagcaaca aggaattacg
    13441 ttggttagcg agcaaaaaaa atcttgtgat tgcagtacac aagtcccaaa cggctacctt
    13501 gttgcatacc gattcgcagc aacaggttcg tggtattaat gctgattatt taaatctttt
    13561 aaaaagagcg ttaaatatca aattaacact ccgggaatac gccgatcatc aaaaagcaat
    13621 ggacgcgctt gcagaaggtg aagtcgatat agtgttatca catttagtta cttcgccgcc
    13681 tcttaatagt gacattgctg caaccaaacc attgataatt acctttccgg cgctggtaac
    13741 cacccttcac gactcaatgc gaccgcttac ctcaccaaaa ccagtaaata ttgctcgggt
    13801 agcaaattac cccccagacg aggtaattca tcaatcgttt ccaaaagcaa caattatctc
    13861 ttttacaaat ttatatcagg cattagcatc cgtatcagcc ggacagaatg attactttat
    13921 tggtagtaac atcattacca gcagtatgat ttcccgctat ttcactcact ccttaaatgt
    13981 agtgaaatat tataactcgc cgcgtcaata taattttttc ttgaccagaa aagaatccgt
    14041 cattcttaat gaagtactca atagatttgt tgatgcttta acaaatgaag ttcgctatga
    14101 agtttcacaa aattggcttg atacaggaaa cctggccttt ctgaacaaac cattagaact
    14161 cactgaacat gaaaaacagt ggattaagca acatcccgat ttaaaggtgc tggaaaatcc
    14221 ttactctcct ccctattcta tgacggatga aaatggctcg gttcggggcg ttatggggga
    14281 tattcttaat attattacct tgcaaacagg tttaaatttt tctccgatca ccgtttcaca
    14341 caatatccat gccggaacac agcttaaccc cggtggatgg gatataatac ctggcgctat
    14401 ttatagtgaa gatcgagaaa ataatgtttc atttgcagaa gctttcataa caacgccata
    14461 cgtttttgtc atgcaaaaag cacctgacag tgaacaaaca ttaaaaaaag gaatgaaagt
    14521 tgccattcca tattattatg agctgcattc gcaattaaaa gagatgtatc cggaggttga
    14581 atggatacag gtcgataatg ccagcgctgc atttcacaag gttaaggaag gtgaacttga
    14641 tgctctggtc gcgacacagt taaattcgcg ttacatgatc gatcattact atcctaatga
    14701 actttatcat tttcttattc ctggcgttcc gaatgcatcg ctttcgttcg cttttcctcg
    14761 cggagaaccg gaacttaagg atattattaa taaagcactg aatgcaattc ccccaagcga
    14821 agttctgcgc ctgacggaaa aatggattaa aatgcccaat gtgaccattg acacatggga
    14881 cctatatagc gagcaatttt atattgttac gacattatcc gttttattag ttggcagtag
    14941 ccttttatgg ggattctacc tgttacgctc agttcgtcgt cgtaaagtca ttcagggtga
    15001 tttagaaaac caaatatcat tccgaaaagc actctcggat tccttaccga atccaactta
    15061 tgttgtaaac tggcaaggta atgtcattag tcataatagt gcttttgaac attatttcac
    15121 tgcggattac tacaaaaatg caatgttacc attagaaaac agtgactcac cctttaaaga
    15181 tgttttttct aatgcgcatg aagtcacagc agagacgaaa gaaaatcgaa caatatacac
    15241 acaggtattt gaaattgata atggcatcga gaaaagatgc attaatcact ggcatacatt
    15301 atgcaatctt cctgcaagtg acaatgcagt atatatttgt ggttggcaag atattactga
    15361 aacgcgtgat ctaattaatg cactcgaggt agaaaaaaat aaagcgataa aggctaccgt
    15421 agcaaaaagt cagtttctgg caacgatgag tcacgaaata agaacaccaa taagctctat
    15481 tatgggcttc ctggaacttc tgtcgggttc tggtcttagc aaggagcaac gggtggaggc
    15541 gatttcactt gcctacgcca ccggacaatc actcctcggc ttaattggtg aaatccttga
    15601 tgtcgacaaa attgaatcgg gtaactatca acttcaacca caatgggtcg atatccctac
    15661 tttagtccag aacacttgtc actctttcgg tgcgattgct gcaagcaaat cgatcgcatt
    15721 aagttgcagc agtacatttc ctgatcatta cctggttaag atcgaccctc aggcgtttaa
    15781 gcaggtctta tcaaatttac tgagcaatgc tctcaaattt accaccgagg gggcagtaaa
    15841 aattacgacc tccctggttc acattgatga caaccacgct gttatcaaaa tgacgattat
    15901 ggattctgga agtggattat cgcaggaaga acaacaacaa ctctttaaac gctacagcca
    15961 aacaagtgca ggtcgccagc aaacaggatc tggcttaggc ttaatgatct gcaaagaatt
    16021 aattaaaaat atgcagggcg atttgtcatt agaaagtcat ccaggcatag gaacaacatt
    16081 tacgatcaca atcccggtag aaattagcca gcaagtggcg actgtcgagg caaaagcaga
    16141 acaccccatc acactacctg aaaagttgag catattaatc gcggatgatc atccgaccaa
    16201 caggctatta ctcaaacgcc agctaaatct attaggatat gatgttgatg aagccactga
    16261 tggtgtgcaa gcgctacaca aagtcagtat gcaacattat gatctgctta ttactgacgt
    16321 taatatgccg aatatggatg gttttgagtt gactcgcaaa ctccgtgagc aaaattcttc
    16381 cttacccatc tgggggctta cagccaacgc acaggctaac gaacgtgaaa aagggttaag
    16441 ttgcggcatg aacttatgtt tgttcaaacc gttgaccctg gatgtactga aaacacattt
    16501 aagtcagtta caccaggttg cgcatattgc acctcagtat cgccaccttg atatcgaagc
    16561 cctgaaaaat aatacggcga acgatctaca actgatgcag gagattctca tgactttcca
    16621 gcatgaaacg cataaagatc tacccgctgc gtttcaagca ctagaagctg gcgataacag
    16681 aactttccat cagtgtattc atcgcatcca cggtgcggct aacatcctga atttgcaaaa
    16741 gttgattaat attagccatc agttagaaat aacacctgtt tcagatgaca gtaagcctga
    16801 aattcttcag ttgctgaact ctgtaaaaga acacattgca gagctggacc aggagattgc
    16861 tgttttctgt cagaaaaatg actaaatagc ggctcccaca atgttcaaat gtgggagcta
    16921 tttaccagca catcttacat ttatgatgag aactcctggc gaatttgttc cccatgctgg
    16981 tcgagcgttg ccgctcccgg cataacatgc gggtccgcgc agccgctgat ttttatcgga
    17041 ttacccggca tcattattcc cccggcttca atcaacatat ttctcgcctg agtttgtggc
    17101 aatttaatgg cctcagccac acttaataac ggcgcgacgg gtacaccaac ttcatgtatt
    17161 ctggctaacc aaacttcagc tgcctgcgtt tttaacgtcc gctcaatata ttgtttaaga
    17221 atagcctggt tttgtacgcg taaaatattg ctgctaaatc ggggatcatt aaccagttcc
    17281 gtaagctcca gtgcctggca taacgcagaa aaaagcttgt cattaccaca acaaatcgta
    17341 atcggcttat cctgagtatt gaaaacatca aaaggtgcca tgtagggatg gcgatttccc
    17401 agacgttgtg gtgacttccc agttgcgata tatgccatca gaccatgctc cagaaaactc
    17461 agcgtggcat caaacatcgc tatatcgaca tgcgcccctc tctggctctt ttcgcggcca
    17521 taaagtgcac tcactattcc gctgaataaa tagacaccgc cgcatagatc cgcaagagag
    17581 gtaccaacgc gcactggcgg agcatcagga tatcctgttt ccatcattat cccgctcatt
    17641 gcctgaatga tggtatcgta ggcaggagca tcttttagcg gaccggtatg tccgaaacct
    17701 gacgatgaag catatatgag gcgcgggttg atttcttgaa gcgtttccca tgaaaacccc
    17761 agtttttcca ttgtacctgg gcgaaaattc tcagctaata catcagcttg tttgagcata
    17821 tttataaata tacttttatc gtgatcattc tttaaatcaa gaaccacact ctctttgcca
    17881 tgattaataa aactgtaata gagtgactgt ccatccacat agggaccaaa tgtgcgggta
    17941 tcatcaccat gacccggcgg ctcaacttta attacccttg cgcccatatt acaaagaagt
    18001 tgagttccga aaggtccatt aaggacatgt gtcatatcga taactaataa gccttcaaac
    18061 ggccctttgc tttcattatt tgtcatttgc ttatgccttg aaaaaagccg aaggtgaaaa
    18121 gacacttcgg catttatgta aaatcaataa catgattaat tatgaaacca gtcgactgac
    18181 ataaatccac aagggagccg tgacgacaaa gcccagtacg ctcacagcca atgacgctgt
    18241 accagtacgg gtgtagacat taaaccggct ggcaataatg attccggaga atgccggcgg
    18301 taatgcgcct gccagtacca tcatctgcag atgttcgctg ttcaaatgac atgccatacc
    18361 aacgagaagc agtgccagtg gcatcagaat cagcttcagg aaggtgttat aagcaatttc
    18421 ggcactgaac tcgaatttat gtgcagccag agtcaacccc gcagcgaata ccgctacccc
    18481 tgagttagcc ttcgcaatca gattaaaggt tgggtcccat gctgccggaa tttttacccc
    18541 aaccaacacc aggatcgttg ccagaacagg tgcccatact actggctcct ttgccgcaga
    18601 aattaatgcg ctcagattac tattcttctt accatccgct cctgaggaag gattcagcaa
    18661 atacagacca ataggaatag taattgcgtt aacaataata gaaataattg ctaccactaa
    18721 acctgttgat acggaatcac cataaatagg atcgagaact gcaaacccca ggaatccaat
    18781 ggtaggtgaa cccgcaatta atgcacatac agctgcttct gcatgggtac gtttaaaaaa
    18841 tttgtagcaa ccaaaccagg agaaaaagaa acatccgaca ataaccacaa gtgatacaag
    18901 ggtcagacga gtgtccgcaa aaatcatttc cctgtttgcc cgggtaatag aaacaaatag
    18961 agccgcagga agagcgtagt ttaataccag tttattaaat gcgcgggctt gatcttctga
    19021 aaaagtttct cgtctgccgc taaaataacc caacagcatg attacgataa taggcaataa
    19081 atcgccaata aaaaatgtta gcataatagt atttccgata agggtcccct caccatcgca
    19141 taatgagttg gcaagaggag acgatgtcag tatttaatta ccagcgactt gttttgggtt
    19201 aagtttggta atatggccac tttcagttcc tgctgcaggg tcgatgacca cattaataat
    19261 ggtcggtttg cgcgactgaa taccggtggt taaagcatga cgaagttcat ctgtcgtggt
    19321 gacgttatag ccaacgccac gaaacgcatc cattaatttg tcataccttg catggtgcaa
    19381 cagatccgtt ggtgatggtg caccagcgcc actgagatca acaccgtctc ctctgtagat
    19441 gccgccatta ttaaaaataa cgatcgtcac cggcaggtta tatcgacaaa tcgtttcaat
    19501 ttccatccca ctgaaaccaa aagcactatc accttcaatg gcgacaaccg gagaaccaga
    19561 agtcacgcta gcaccgatgg catagcccat accgatgccc atgacacccc aggtaccaca
    19621 atccagacga cgacgtggtt tatacatatc aataatattt cgtgcattat ccagggtatt
    19681 tgcaccttca ttaactaaat aaatatcctg gttctcgcgc aatacgtcgc gcacagcact
    19741 taatgcatta aagtaattta atggttgagt atctgtactt aatttttcat gcattttttg
    19801 tgcattttgc tgcttgtgga tatttaaaat atcgcgccat accagtggag tcgtaaatgt
    19861 gttttgtttc agttctgcca gcataccttg catactggat gcaatatcac caacgactgg
    19921 cacagcaatg gggcggttgc tgtcaatttc ctgcggttca atatccagtt gaataaactg
    19981 tgtatctgcc gcccatcctt ttttaccgtg tgccaataac caattcagtc gtgcaccaac
    20041 aagcatgaca acgtcagcat ttgccagggc aaacgaacgc gcagctgccg cagaaagtgg
    20101 atgcgtatct tcaaggatcc ctttcgccat agacattggc aggaatggaa tctgagtact
    20161 ttcaataaat tcacgaagct gttcatcagc ttgtgaatac gccgcgcctt tgccaaggat
    20221 aattaatggc cgttcagctt ttgctaaaag cgaaattgcg ctagtgactg acttcgggca
    20281 tggcaataat gctggcgacg gattttcaac tttaacaatc gtggttaacg cttcgtcttt
    20341 ttccatcgtc gcggccagga catttgctgg caaatcaaga taaactccgc cagggcgacc
    20401 cgatacagag acccggatag cgcgtgccaa tgcaatgcca agatcctgcg gctgattaac
    20461 gcgaaatgct gctttggcat acggttttgc cgcattcatt tggtccagct cttcataatc
    20521 accttgctgt aggtcgacga tcgcgcggtc gctggagccg ctaatcatga tcatcggaaa
    20581 accatttacc gttgcgttgg ccaatgcggt caaaccattg aggaatcctg gcgcagaaac
    20641 tgtcaggcag atccccggtt tttgggtaag aaaaccgctt gccgcagcgg catagcctgc
    20701 cgactgctca tgacgaaaac caatataacg aatgccttcc gcctgggcat ggcgtgccat
    20761 atccgtcaca ggaataccta caacaccata aatagtgtca atattattct gttttaatgc
    20821 ttcaacgatg atatgcatac catctgtcat ttgaagttga tctgacatga ttaataacct
    20881 cgaaattttt ataaattggg agccagaagc ccccgttaat atcagatggc gtggttttgc
    20941 ttcattgcag caatttcatc gtcgctataa cccagctcct gcaatacagc agcggtatgt
    21001 tcacctaata gcggcgcagc tttaatatcc ggcgtaaagg cagagaattt cattggacaa
    21061 ccaacggtca gatattttcc acgcaacggt tgttccactt caacaacact gccactttgg
    21121 cgcaaagagg gatcaagtga aatttctttc atacttaaaa ccggtgcaca aggaatatca
    21181 aactgagtca aataggccac cgcttcatgt ttatcaatag tgacagtgta tttttcgatt
    21241 tcagcaaaaa tatcgaaaat atgtggctgt cgtgcatggg ctgtactgta tgccggatcg
    21301 gtaatccatt ctggtttgcc gatggcttta caggtgtttt cccagttttg ctcctgaata
    21361 gtgaaataaa tataggcgtt aggatcggtt tcccagcctt tacatttcag gatccagcca
    21421 ggctgaccgc caccacctgc attaccaccg cggggaactg catcaccaaa tgtaccattc
    21481 ggatactgcg ggtattcttc cagataaccc aatttatcga gacgctgctg gtcacgtaat
    21541 ttcacgcggc aaaggttcaa tacggcatcc tgcattgaca tggtgactcg ttgcccacgc
    21601 cccgtttttt cgcgatgcag caaagcagca agtaaaccga tcagcaaatg cattccggtg
    21661 ttgctgtcac ccaacgctgc agcgcttacc agcggcggac catcccaaaa acccgtagtg
    21721 gatgccgcgc cacccgctgc ctgagcaacg ttttcatagg cttttacatt cacataaggc
    21781 gaacactcat caaacccttt gatcgaacca aaaatcagac gtggattgat ttcttgaata
    21841 tgctcccagg tgaagcccat gtgatcaatg gcccctggat gaaagttctc gactaagata
    21901 tcagcttcgc ggatcagctt ttccattacc tctttgcctt ccgctgtttt ggtatttaac
    21961 tcaatagaac gtttgttact gttaagcatg gtgaagtaaa gcgcatcgat atcaggaata
    22021 tctcgcagct ggtgacgcgt tacgtcacca acgccgggac gttcaatttt aataacgtca
    22081 gcgccaaacc aggccagcat ttgagtacaa gatgggccag attgcacacc ggtgaaatcg
    22141 agaactttaa ttccttgaag tggagttgac atagcaatac ctattagtta attaaatatg
    22201 tgttaattta gcgtttattt aaggcgtaat atcttctata aataacccgc aaaatatcgt
    22261 cgtagctgca ctcgatattt ttaaaatcct gtatcaagtg ctgaaaatca tttcatcgcc
    22321 acttccaatt cctttctgca tggtaagtag tgaggacgaa aagaagaata tgataaatat
    22381 tgattcgtgg taatcagaga gagtcgcgac atttgtgatg ttattcttac aactgtaaga
    22441 agcgccggtg atttacaagt aagggtatga ttacgctttt taaagcgtac cataaaacat
    22501 aacataataa atgatttgtg atgacacggg attattggga tttactttga ggaagataaa
    22561 atgcgtgttt atagaatcgc gagcgccaaa taattttatg accacaacct gtcgtattgt
    22621 ggtcataaaa aattgtcggc tggagggagt taaatccttt acctcaatat actgcctgtc
    22681 agcattagtt cacaaacagt gcttcgctgt cgacgatgat tccatcttcc gcacctttaa
    22741 gcgccaggtt tgcctcgtag tattgctttt tatcaagcag tttctgcgca tcagcaagtg
    22801 cattacgagt ctgtttaagc ggcatcagat attggttttc cattacgcca acaccggcca
    22861 ggcgaagttc ttccattgcg ccctttttat cccctttcgc cattttttca ttggcaattt
    22921 ttatcgcggc ttctttttcg ggagttgcta cataactctc agaaattccg acagatgcat
    22981 taatgacaat atattggtca tcattgagat tggtcttttt acctggctta gcgaactttg
    23041 cccattcggt actgtcatct gacagcaaag cggaagcttc attggtcagt tcttttgcct
    23101 tttctggatc gccatgaaac agagccaggc gcgctacctg cacgtcacgc atcgcatata
    23161 aaccttgttc agagatacgc tctgctgctg ccgttttctg ggtttgctga acttgctgag
    23221 tttgctgttg tgccgctacc ggtgcattat cagcagccca tacagttgaa gatgccagaa
    23281 ttgctgtgac catcgtagcc ataattaaac gtttcatact tacctcattc tttaacagtt
    23341 ttagaaaacg ccgccatcat attgggtgag catgaaatga gaaatcagag agcaacggtc
    23401 attttgaaag ggttatctta cagttgtagt tattatcgca agaagtattt tagtcttaca
    23461 attgtaggaa tctgcttcct gtcgcagaga actattctta tgta
//
LOCUS       DAZFXT010000073        22366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.073, whole genome shotgun sequence.
ACCESSION   DAZFXT010000073 DAZFXT010000000
VERSION     DAZFXT010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.073"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            157..840
                     /locus_tag="ACHKW0_003033"
     CDS             157..840
                     /locus_tag="ACHKW0_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HFO7475830.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            884..5734
                     /gene="ehaG"
                     /locus_tag="ACHKW0_003034"
     CDS             884..5734
                     /gene="ehaG"
                     /locus_tag="ACHKW0_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HFO7475831.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTN
                     DDGLAFNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIA
                     KGSRDTSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANAL
                     NGIAIGSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSS
                     TNQRRITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            6102..7757
                     /gene="lldP"
                     /locus_tag="ACHKW0_003035"
     CDS             6102..7757
                     /gene="lldP"
                     /locus_tag="ACHKW0_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HFO7475832.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCIVGVITTLQAYVLTWMIP"
     gene            7757..8533
                     /gene="lldR"
                     /locus_tag="ACHKW0_003036"
     CDS             7757..8533
                     /gene="lldR"
                     /locus_tag="ACHKW0_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HFO7475833.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFSGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8530..9720
                     /gene="lldD"
                     /locus_tag="ACHKW0_003037"
     CDS             8530..9720
                     /gene="lldD"
                     /locus_tag="ACHKW0_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HFO7475834.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALGPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSINEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            9906..10379
                     /gene="trmL"
                     /locus_tag="ACHKW0_003038"
     CDS             9906..10379
                     /gene="trmL"
                     /locus_tag="ACHKW0_003038"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HFO7475835.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10432..11253)
                     /gene="cysE"
                     /locus_tag="ACHKW0_003039"
     CDS             complement(10432..11253)
                     /gene="cysE"
                     /locus_tag="ACHKW0_003039"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HFO7475836.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11333..12352)
                     /gene="gpsA"
                     /locus_tag="ACHKW0_003040"
     CDS             complement(11333..12352)
                     /gene="gpsA"
                     /locus_tag="ACHKW0_003040"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HFO7475837.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12352..12819)
                     /gene="secB"
                     /locus_tag="ACHKW0_003041"
     CDS             complement(12352..12819)
                     /gene="secB"
                     /locus_tag="ACHKW0_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HFO7475838.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12882..13133)
                     /gene="grxC"
                     /locus_tag="ACHKW0_003042"
     CDS             complement(12882..13133)
                     /gene="grxC"
                     /locus_tag="ACHKW0_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HFO7475839.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13275..13706)
                     /locus_tag="ACHKW0_003043"
     CDS             complement(13275..13706)
                     /locus_tag="ACHKW0_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HFO7475840.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            13951..15495
                     /gene="gpmM"
                     /locus_tag="ACHKW0_003044"
                     /gene_synonym="pgmI"
     CDS             13951..15495
                     /gene="gpmM"
                     /locus_tag="ACHKW0_003044"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006007 - glucose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HFO7475841.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVEGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15529..16788
                     /gene="envC"
                     /locus_tag="ACHKW0_003045"
     CDS             15529..16788
                     /gene="envC"
                     /locus_tag="ACHKW0_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418070.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HFO7475842.1"
                     /translation="MTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADERDQLKSIQ
                     ADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNKQIDEMNAS
                     IAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYFGYLNQARQ
                     ETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKKTLAGLESS
                     IQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEATRKGTTYK
                     PTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIGASEGTEVK
                     AIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQPIALVGSS
                     GGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16792..17751
                     /locus_tag="ACHKW0_003046"
     CDS             16792..17751
                     /locus_tag="ACHKW0_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HFO7475843.1"
                     /translation="MFPFRRNVLAFATLLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17738..18772)
                     /gene="waaH"
                     /locus_tag="ACHKW0_003047"
     CDS             complement(17738..18772)
                     /gene="waaH"
                     /locus_tag="ACHKW0_003047"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HFO7475844.1"
                     /translation="MMNSTSKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(19011..20036)
                     /gene="tdh"
                     /locus_tag="ACHKW0_003048"
     CDS             complement(19011..20036)
                     /gene="tdh"
                     /locus_tag="ACHKW0_003048"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HFO7475845.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(20046..21242)
                     /gene="kbl"
                     /locus_tag="ACHKW0_003049"
     CDS             complement(20046..21242)
                     /gene="kbl"
                     /locus_tag="ACHKW0_003049"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HFO7475846.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(21517..>22366)
                     /gene="yibB"
                     /locus_tag="ACHKW0_003050"
     CDS             complement(21517..>22366)
                     /gene="yibB"
                     /locus_tag="ACHKW0_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312523.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HFO7475847.1"
                     /translation="STTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLAALE
                     NEMIIFTSPDLKPRVEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRLEPR
                     QLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLKIWD
                     FPFDESKMHLFTIKKGLTVTSQQQVFDFMIGNHVYIIGGAIVGSQHKWKEFYKLVLES
                     QKITLNNNIVDDDQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQALR
                     IFLSRK"
BASE COUNT         5705 a         5758 c         5657 g         5246 t
ORIGIN      
        1 tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa tcgacacaca
       61 tcactgtcat taatgtaaag caacatagct caccaataat acaatggaca actctaccgg
      121 cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa gatttttttc
      181 agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat tgaagaaggc
      241 aagccagaaa gtagtttact gaaacaacaa gaagaacata ataataaaat tgtgctactt
      301 gaaaaacaac aagcacagct aaagagtcaa ctcgaaacta ttcaaaaaca acaaactggc
      361 ataataaaca gtacaaagac tttaactcac gtaattaaat ccgttaaaga ccaacaaaat
      421 acttttattt ttaccgagtt taacccagca aaaacgaagt atttcatcct gaacaacggt
      481 tcggtggctt tagcgggtcg agtgttatcc attgacgcca cagagaatgg tagtgttatt
      541 catatctcac tggtcaactt attaagtaca cctatctcaa acattggttt taatgcgaca
      601 tggggtggtg aaaaacctgt cgatgccaaa gagtttgccc ggtggcaaca attacttttc
      661 aacacatcaa tgaaatccac attgaaatta ttaccaggtc aatggcaaga cattaatttg
      721 accctgaagg gtgtatcgcc caataacctg ggatatctga agttagccat caacatggaa
      781 aatattcagt tcgacaatct tccctctgct gaaaatcggc agaaaagaag caaaaaataa
      841 atgcagttag tacgcttata acaaggaata gcgagcatca ttaatgaaca aaatatttaa
      901 agttatctgg aaccctgcga cagggaatta tactgttacc agcgaaacgg caaaaagccg
      961 tggcaagaaa tctgggcgca gtaagctgtt aatttctgcg ctggttgcgg gtggaatgtt
     1021 gtcgtcgttt ggggcattgg cgaatgccgg gaatgacaac ggtcagggtg ttgattacgg
     1081 tagtggatca gctggcgacg gctgggttgc tataggcaaa ggggcgaaag caaatacttt
     1141 tatgaacacc agtggttcca gtactgctgt gggttatgac gctatagctg aaggccaata
     1201 tagctctgcc atcgggtcaa aaacccatgc gattggcggt gcatcaatgg cctttggggt
     1261 tagtgcaata tcagaaggcg atagaagtat agcactgggt gcctcttcgt attcattggg
     1321 ccaatactca atggccctcg gccgttattc aaaagcattg ggtaaattgt ctattgctat
     1381 gggggactct tccaaagcgg aaggagcaaa cgccattgcc ctgggaaatg ccactaaagc
     1441 tactgagatt atgagtattg ctcttggcga caccgccaat gcgtcaaaag cgtattcaat
     1501 ggcgctggga gcaagtagcg tcgcatctga agaaaacgca attgccctgg ggcgtagcag
     1561 tgtagctagc ggtactgaca gcctcgcatt tggcagacaa tcacttgcca gcgcagcgaa
     1621 cgctattgcg ataggtgctg agaccgaagc cgctgaaaat gcaactgcta ttggcaataa
     1681 tgcgaaggca aaagggacta atagcatggc aatggggttc ggaagccttg ccgataaagt
     1741 caatactatc gcattaggaa atggcagcca ggctctggca gataatgcaa tcgccatagg
     1801 ccagggcaac aaagctgatg gcgtggatgc catcgctctg ggtaatggta gccagtcgag
     1861 aggcttaaac accattgcct taggcacagc cagtaatgca actggtgata agagtcttgc
     1921 gcttggtagt aatagcagtg ccaacggtat taactctgtc gcgctgggcg cagattccat
     1981 tgcggattta gacaataccg tctctgtcgg caatagttca ttaaaacgca agatcgttaa
     2041 tgtgaaaaat ggcgcgatca agtctgacag ttacgatgcc attaatggtt cacagcttta
     2101 tgccattagc gactcggtag caaaaaggct tggaggaggg gctgcagtag atgttgatga
     2161 cggtactgtt acagcaccaa cctacaattt aaaaaatggt agcaaaaata acgtaggggc
     2221 tgcgctcgct gtacttgatg aaaacaccct gcaatgggac caaaccaaag gcaaatacag
     2281 cgctgctcat ggtactagta gcccaactgc cagcgtaatc accgatgttg cggatggcac
     2341 gatttcagcc tccagtaagg atgcggttaa cggttcccaa ctgaaagcta ccaatgacga
     2401 tgtcgaagcc aacaccgcca atatcgctac taataccagc aacattgcca cgaatacggc
     2461 aaatattgcc accaatacca ccaatatcac caacctgacg gattccgttg gtgaccttca
     2521 ggctgatgcc ctgctctgga acgaaactaa aaaggcattc agtgcagctc acggccagga
     2581 taccaccagc aaaatcacca acgttaaaga tgccgacctg acggctgaca gcactgatgc
     2641 tgttaacggc tctcagctga aaaccaccaa cgatgctgtg gcgacgaata ccaccaatat
     2701 cgccaataac acttccaata ttgccactaa caccaccaac atctctaacc tgactgagac
     2761 ggtgactaat cttggtgagg atgcgctgaa atgggataag gacaatggtg tattcacggc
     2821 agctcatggc accgagacca ccagcaaaat caccaacgtt aaagatggcg acctgacgac
     2881 tggcagcacc gatgccgtta acggctctca gctgaaaacc accaacgatg ccgtggcgac
     2941 gaataccacc aatatcgcca ctaacaccac caacatctct aatctgactg agacggtgac
     3001 taatcttggt gaggatgcgc tgaaatggga taaggacaat ggtgtcttca ctgcagctca
     3061 tggcaacaat accgccagca aaatcaccaa tatcctggac ggcacagtca ctgcaaccag
     3121 ttccgatgcc attaacggta gccagcttta tgacttaagc agcaatatcg ccacctactt
     3181 cggcggcaat gcttctgtga atactgacgg tgtgtttacc ggtccaacct acaaaatcgg
     3241 tgaaacaaat tattataacg tcggcgatgc actggctgcg attaactcct catttagcac
     3301 gtctctcggc gatgctctgc tttgggatgc caccgcaggt aaattcagtg ccaaacacgg
     3361 tactaatggt gacgcaagcg tgatcactga tgtcgcagat ggtgaaattt cagactccag
     3421 ttctgacgca gtaaacggct cacaactcca cggcgtgagc agttatgttg ttgatgcgct
     3481 ggggggtggt gccgaagtca atgcagacgg caccatcact gcgccgacgt acaccattgc
     3541 taatgctgat tacgataatg tcggtgatgc cctgaatgct atcgatacca ctcttgacga
     3601 cgctctgctc tgggatgcgg acgccggtga aaatggtgca tttagcgccg ctcacggaaa
     3661 agataaaact gccagtgtaa tcactaacgt cgctaacggt gcaatctctg ctgccagcag
     3721 cgacgcgatt aacggctcac aactctatac caccaataag tacatcgctg atgcgctggg
     3781 tggtgacgca gaagtcaacg ctgacggcac catcaccgca ccgacttaca ccattgcgaa
     3841 cgccgagtac aacaacgtcg gtgacgccct ggatgcgctt gatgataacg ccctgctgtg
     3901 ggatgagact gccaatggcg gtgctggagc ctacaatgcc agccatgacg gtaaagccag
     3961 catcatcact aatgtcgcta atggcagtat tagtgaggac agtaccgatg cagtgaacgg
     4021 ttctcagttg aatgcgacga atatgatgat tgagcagaac acccaaatta tcaatcagct
     4081 cgctggtaac accgacgcaa cctatatcca agaaaacggt gcgggtatta actatgtgcg
     4141 tactaacgac gacggcttag cgttcaacga cgccagcgca cagggtgttg gcgctacagc
     4201 tataggttat aactctgtcg ccaaaggcga tagcagcgta gctattggtc agggcagcta
     4261 cagcgacgtt gatacgggta tcgccctggg tagcagctct gtttccagcc gagtgattgc
     4321 caaaggctcc cgtgacacca gcataacgga aaatggcgtt gttattggtt acgacaccac
     4381 ggatggcgaa ctgctcggtg cattgtctat cggtgatgac ggtaaatatc gtcaaatcat
     4441 caacgtagcc gatggttccg aagcccatga cgccgttacg gttcgtcaat tgcagaatgc
     4501 gattggtgcg gtcgcaacca cgccgactaa atacttccac gctaattcaa cggaagaaga
     4561 ttcactggca gtgggaactg actcgctggc aatgggtgcg aaaaccatcg tgaatggcga
     4621 taaaggtatt ggtatcggtt atggtgccta cgtggacgcg aatgcactta acggcattgc
     4681 cattggtagc aatgcgcaag tcattcatgt caacagtatt gcgataggta atggttctac
     4741 gaccactcgt ggcgctcaaa ccaattatac cgcctacaac atggacgcac cgcagaactc
     4801 tgtcggtgaa ttctcagtcg gtagtgcgga tggtcaacgt cagatcacta acgtcgcagc
     4861 aggttcggct gataccgatg cggtcaacgt gggtcagttg aaagtaacgg atgcgcaggt
     4921 ttcccagaat acccagagca ttactaacct ggataatcgg gtaacgaatc ttgattcacg
     4981 cgtcaccaat atcgaaaacg gtattggcga tatcgtcacc accggtagca ccaagtactt
     5041 caagaccaat accgatggtg tagatgccag cgcgcagggt aaagatagcg tcgcgattgg
     5101 ttctggctcc attgctgccg cggataacag cgtcgcactg ggtacagggt ctgtggcaac
     5161 cgaagaaaat acgatctctg taggctcatc tactaatcaa cgtcgtatca ccaacgtagc
     5221 cgcaggtaaa aacgacaccg atgctgttaa cgtggcacag ttgaagtctt ccgaagctgg
     5281 cggtgtgcgt tacgacacca aagctgatgg ttctatcgac tatagcaata tcaccctcgg
     5341 tggcggcaac ggcggtacga ctcgtatcag caacgtctcc gctggcgtca acaacaacga
     5401 cgcggtgaat tacgcgcagt tgaagcaaag cgtgcaggaa acgaagcaat acaccgatca
     5461 gcgaatggtt gagatggata acaaactgtc taaaactgaa agcaagttga gcggtggtat
     5521 cgcttctgca atggcaatga ccggtctgcc gcaggcttat acaccgggtg ccagcatggc
     5581 ttctattggt ggcggtactt acaacggtga atcggcagtt gctttaggtg tatcgatggt
     5641 gagcgccaat ggtcgttggg tctacaaatt acaaggtagt accaatagcc agggtgaata
     5701 ctccgccgca ctcggtgccg gtattcagtg gtaatgatcc attaagttag tgtgactaag
     5761 ggcaaacgcc aaattgcctg atgcgttacg cgcatcaggc aagataaggc gctccgggtc
     5821 aacaatctaa gggcaattct ctgatgagga ttgccctttt ctttaccaga catctccccc
     5881 cacaagaatt ggccctacca attcttcgct tatctgacct ctggttcaca attccccaat
     5941 taaaactcac atcaatattg ccaatacata acatttagtt aaccattcat tgtcattatc
     6001 cctacacaac acaattggca gtgccacttt tacacaacgt gtgacaagga gatgagcaac
     6061 agactcatta cacgatgtgc gtggactcca ggagacctgc aatgaatctc tggcaacaaa
     6121 actacgatcc cgccgggaat atctggcttt ccagtctgat agcatcgctt cccatcctgt
     6181 ttttcttctt tgcgctgatt aagctcaaac tgaaaggata cgtcgccgcc tcatggacgg
     6241 tagcaatcgc ccttgccgtg gctttgctgt tctataaaat gccggtcgct aacgcgctgg
     6301 cctcggtggt ttatggcttc ttctatggtt tgtggcccat cgcgtggatc attattgcag
     6361 cagtgttcgt ctataagatc tcggtgaaaa ccgggcagtt tgacatcatt cgctcgtcta
     6421 ttctttcgat aacccctgac cagcgtctgc aaatgctgat cgtcggtttc tgtttcggcg
     6481 ctttccttga aggagccgca ggctttggcg caccggtagc aattaccgcc gcattgctgg
     6541 tcggcctggg ttttaaaccg ctgtacgccg ccggactgtg cctgattgtt aacaccgcgc
     6601 cagtggcatt tggcgcgatg ggcattccaa tcctggttgc cggacaagta acaggcatcg
     6661 acagctttga gattggccag atggtggggc ggcagctacc gtttatgacc attatcgtgc
     6721 tgttctggat catggcgatt atggacggct ggcgcggtat caaggagacg tggcctgcgg
     6781 tcgtggttgc gggcggctcg tttgccatcg ctcagtacct tagctctaac ttcattgggc
     6841 cggagctgcc ggacattatc tcttcgctgg tatcactgct ctgcctgacg ctgttcctca
     6901 aacgctggca gccagtgcgc gtattccgtt ttggtgattt gggggcgtca caggttgata
     6961 tgacgctggc ccacaccggt tacactgcgg gtcaggtgtt acgtgcctgg acaccgttcc
     7021 tgttcctgac agctaccgta acactgtgga gtatcccgcc gtttaaagcc ctgttcgcat
     7081 cgggtggtgc gctgtatgag tgggtgatca acattccggt gccgtacctc gataaactgg
     7141 ttgcccgtat gccaccagtg gtcagcgagg ctacagccta tgccgccgtg tttaagtttg
     7201 actggttctc tgccaccggc accgccattc tgtttgctgc actgctctcg attgtctggc
     7261 tgaagatgaa accgtctgac gctatcagca ccttcggcag cacgctgaaa gaactggctc
     7321 tgcccatata ctccatcggt atggtgctgg cattcgcctt tatttcgaac tattccggac
     7381 tgtcatcaac actggcgctg gcactggcgc acaccggtca tgcattcacc ttcttctcgc
     7441 cgttcctcgg ctggctgggg gtattcctga ccgggtcgga tacctcatct aacgccctgt
     7501 tcgccgcgct gcaagccacc gcagcacaac aaattggcgt ctctgatctg ttgctggttg
     7561 ccgccaatac caccggtggc gtcaccggta agatgatctc cccgcaatct atcgctatcg
     7621 cctgtgcggc ggtaggcctg gtgggcaaag agtctgattt gttccgcttt actgtcaaac
     7681 acagcctgat cttcacctgt atagtgggcg tgatcaccac gcttcaggct tatgtcttaa
     7741 cgtggatgat tccttaatga ttgttttacc cagacgcctg tcagacgagg ttgccgatcg
     7801 tgtgcgggcg ctgattgatg aaaaaaacct ggaagcgggc atgaagttgc ccgctgagcg
     7861 ccaactggcg atgcaactcg gcgtatcacg taattcactg cgcgaggcgc tggcaaaact
     7921 ggtgagtgaa ggcgtgctgc tcagtcgacg cggcggcggg acgtttattc gctggcgtca
     7981 tgacacatgg tcggagcaaa acatcgtcca gccgctaaaa acactgatgg ccgatgatcc
     8041 ggattacagt ttcgatattc tggaagcccg ctacgccatt gaagccagca ccgcatggca
     8101 tgcggcaatg cgcgccacac ctggcgacaa agaaaagatt cagctttgct ttgaagcaac
     8161 gctaagtgaa gacccggata tcgcctcaca agcggacgtt cgttttcatc tggcgattgc
     8221 cgaagcctca cataacatcg tgctgctgca aaccatgcgc ggtttcttcg atgtcctgca
     8281 atcctcagtg aagcatagcc gtcagcggat gtatctggtg ccaccggttt tttcacaact
     8341 gaccgaacaa catcaggctg tcattgacgc cattttttcc ggtgatgctg acggggcgcg
     8401 taaagcaatg atggcgcacc ttagttttgt tcacaccacc atgaaacgat tcgatgaaga
     8461 tcaggctcgc cacgcacgga ttacccgcct gcccggtgag cataatgagc attcgaggga
     8521 gaaaaacgca tgattatttc cgcagccagc gattatcgcg ccgcagcgca acgcattctg
     8581 ccgccgttcc tgttccacta tatggatggt ggtgcatatt ctgaatacac gctgcgccgc
     8641 aacgtggaag atttgtcaga agtggcgctg cgccagcgta ttctgaaaaa catgtccgac
     8701 ttaagcctgg aaacgacgct gtttaatgag aaattgtcga tgccggtggc gctaggtccg
     8761 gtaggtttgt gtggcatgta tgcgcgacgc ggcgaagttc aggcagccaa agcggcggat
     8821 gcgcatggca ttccgtttac tctctcgacg gtttccgttt gcccgattga agaagtcgcg
     8881 ccagccatca agcgcccaat gtggttccag ctttatgtac tgcgcgatcg cggctttatg
     8941 cgtaacgcgc tggagcgagc aaaagcagcg ggttgttcga cgctggtttt caccgtggat
     9001 atgccgacac cgggagcgcg ttatcgtgat gcgcattctg gaatgagcgg cccgaacgcg
     9061 gcaatgcgcc gctatttgca ggcggtgacg catccgcaat gggcgtggga tgtgggcctg
     9121 aacggtcgtc cgcatgattt aggtaatatc tcagcttatc tcggcaaacc gaccggactg
     9181 gaagattaca tcggctggct gggaaataac ttcgatccgt ccatctcatg gaaagacctt
     9241 gagtggatcc gcgatttctg ggatggcccg atggtgatca aagggatcct cgatccggaa
     9301 gatgcgcgcg atgcagtacg ttttggtgct gatgggattg tggtttctaa ccacggtggc
     9361 cgccagctgg acggtgtact ctcttccgct cgtgcactgc ctgctattgc agatgcggtg
     9421 aaaggtgata tcgccattct ggcggatagc ggaattcgta acgggcttga tgtcgtgcgt
     9481 atgattgcgc tcggtgccga caccgtactg ctgggtcgtg ctttcttgta tgcgctggca
     9541 acagcgggcc aggcgggtgt agctaacctg ctaaatctga tcgaaaaaga gatgaaagtg
     9601 gcgatgacgc tgactggcgc gaaatcgatc aatgaaatta cgcaggattc gctggtgcag
     9661 gggctgggta aagagttgcc tacggcactg gctccgatgg cgaaagggaa tgcggcatag
     9721 tcgtttgagc cccctcaccc taaccctctc cctcagggag tggggaccgt tcggcgctgt
     9781 atgtactccc tcactctgaa acgacaccgc actctttttt tctccctcgc ccctccgggg
     9841 tgaggggaaa aggccgcact gctcaccaat tttctgctat cctgcccccg cactaagggg
     9901 gcagtatgct aaacatcgta ctttacgaac cagaaattcc gccaaatact ggcaatatca
     9961 tccgtctttg cgctaatacc ggctttcgtc tgcatatcat cgaaccgatg ggatttgcct
    10021 gggacgataa gcgcctgcgc cgcgcggggc tggactatca cgagtttacc gccgttacgc
    10081 gtcatcatga ctttcgcgcg ttcctcgaag cagaaaatcc ccagcgcctg tttgccctca
    10141 ccacgaaagg tacgcctgct cacagcgccg taaactatca ggatggcgat tatctgatgt
    10201 tcggcccgga aacacgcggc ctgccagcga gcattcttga tgccctgccc gctgaacaaa
    10261 aaattcgcat tccgatggtg ccggacagcc gcagcatgaa cctgtccaat gcggtgtcag
    10321 ttgtggtgta tgaagcctgg aggcagctgg gatatccggg agcgttgttg agagattagt
    10381 tactgtatgc cggatagatg attacatcgc atccggcacg atcacaggac atcagatccc
    10441 atccccatac tcaaatgtat ggttaatacc gttgaaatgc tggtccatat ccattgatgg
    10501 cttatcgctg tctggtttac cgacaatacg agccggaacg ccagcggcgg ttgtatgcgg
    10561 cggcaccggt tgcagcacca cggaacctgc gccaatcttc gcgccgcgac caacttcaat
    10621 attgccgagg attttcgcgc ccgcgccaat catcacacct tcacgaattt tcgggtgacg
    10681 gtcaccacca gatttacccg taccgccaag cgtcacagat tgcagaatcg atacgtcgtt
    10741 ttcaattacc gccgtttcac ccacaacgat gcccgtggcg tggtcgagca taatgccacg
    10801 gccaattttt gctgccgggt gaatatcgac ctggaacgtc acagaaacct ggttttgcag
    10861 aaagattgcc aatgcgcgac gcccctgatt ccacaaccag tgaccgatgc gataggcctg
    10921 caaggcatga aaacccttca ggtataacaa cggggttgag tatttatcga ctgccgggtc
    10981 gcgggtacgc accgcctgaa tatcacaggc cgcagaggcg atcatttccg ggtcagcggc
    11041 gtaggcttct tccaccactt cacggatagc aatagcaggc ataattggcg atgacagctt
    11101 gttcgccagc atgtagctca gtgcactacc aaggttttcg tgcttgagta gcgtcgcgtg
    11161 gtaaaaactg gccagcattg gctcacagtc cgccagcgtt ctggcttcgg ctttaatatt
    11221 gttccagaca atttccagtt cttcacacga cattgcttac tccacacgat gagataatga
    11281 ccggcccgtt ctgcgcgggc cgggtcatag cggtaacaaa ggttccctgg ggttagtggc
    11341 tgctgcgctc gtccttgcgt gcacgaccta ataacgtcaa tgctgcctcg cgcgcgtttt
    11401 ttccgcaata taatacttga taaatttcct cggttattgg catttcaacg ccgaagcgat
    11461 gcgccagttc gcggacttct ttcgtattgc ggtagccttc caccacctga ccaatcttct
    11521 cctgcgcgct ttgtacatcc atgccctgac cgagcatcat gccaaaacgg cggttacgcg
    11581 actggttgtc ggtacaggta agcaccagat cgccaagccc cgccatgccc ataaaggtgg
    11641 caggatcggc acccagcgcc gcaccaagac gcgacatttc agccagccca cgggtgatca
    11701 gcgccgtacg cgcattcgca ccaaaaccga taccgtcgga catccccgcg ccaatggcaa
    11761 tgacgttttt caccgcaccg ccaagctgca caccgatgaa atccgggttg ctgtaaacgc
    11821 ggaaactttt gccacagtgc agcaattgtt ggagatcatc ggcaaaggtc tggtcggtag
    11881 aggccaacga aatagccgtc ggtaaacctg ccgccagttc tttcgcaaac gttgggccag
    11941 agataaccgc cagcggaatt tgatcgccta aggcctcacg cgccacgtcc tgtaacagac
    12001 gtccggtttc cgcttccagc cctttggtcg cccacaccag acgcgcatca ggacgcatca
    12061 gcggtttaat ctggcgcagc acttcaccaa agacatggct gggtacgacg acgagaatat
    12121 tacggctggc tgccagcgca gtggcgagat cgctttcaag atggagcgta tcgggaaaag
    12181 gcacatcggg gagaaacgcg gcgttacagc ggtcgcgttc aagcgttgcg atatgttcag
    12241 ggtcatggcc ccagaggaca acctcgtggc catttcttgc cagggtaatg gcaagagcgg
    12301 tgccgtacga gccggcaccg atcacagtca ttgaagcatt acgttggttc atcaggcatc
    12361 ctgatgttct tcagtacctt cgccagcctg ctgctgcaaa tagttcatga acagcgcatc
    12421 gaagttaacc ggcgcaaggt tcagttgcgg gaatgtaccg cgggatacca tgctggtgat
    12481 gcactcacga gcatacggga acagaatgtt cgggcagtat gcgcccaggc aatgtgccat
    12541 ctgggtgcct tcgatacccg cgatggagaa aataccgccc tgctgaactt cacacaggaa
    12601 cgcggtttct tcgcccaggg aggccgttac ggtaacacgc agtaccactt cgtatacgtc
    12661 atctgccagt tgggaagatg ccgtatccag atcaagttta acttctggtt gccaatcttt
    12721 ctggaaaacg tgcggcgcgt tcggcgcttc gaaagagata tccttggtat aaatacgttg
    12781 gatctggaaa gtcatttcag tgttgttttg ttctgacatg tgtagaaaac ccttaagtgt
    12841 tgtccttaaa tactgcgcaa tgtaatgcca acgttcacac gttatttcag caggggatcc
    12901 agtccaccac gtgcatccaa tgcatacaag tcatcacagc cgccaatgtg ctgtgcgtca
    12961 ataaaaatct ggggaaccgt ggtgcgaccg ctgcgtttga tcatctcttc acgcttggcg
    13021 gcattgccat cgatcggcag ctcctggaaa ctcacgccct tgctgctcag cagtgctttt
    13081 gcacgatggc aatacgggca ggtttctttg gtatagattt caacattggc cataaattag
    13141 ctcctgtttt tttaccctga aatttcatgt tgcagggcgg cagcaaaatg gagtgtcccc
    13201 agaagcttat aacaataagt cgctgggggc ctaattgcag ctaacggcct gcatcatgaa
    13261 agacgacagg taaattattt gccgcgcacc agaggcaagt tttcgccagc ccagccagca
    13321 acgccctctt tcagcacgaa tacttgcgca aaaccagctt tcgtcagcgc gtttgcaggc
    13381 tcctggcact gcatgccaga accgtctacc acgataaccg gtttgtcttt gtgcttctca
    13441 agctcaccaa cattgttggc tttgatttca ctcggcaaca gattgataga acctgcgata
    13501 tggcctttac ggaagtcatc acgctgacgt aaatccacaa ccacagcgtc ttctttgttg
    13561 atcagacgcg tagcttcacc acgagtaatc accttcactt tcgaggtcag gctcttaaac
    13621 gtagtcacaa gaaccgccac cagtaacgcg atccaggcga tactcagtat gggatgacgg
    13681 ccaacaaatt gcataatttc ttgcatgggg ggtaacaact cccgacgtag tgattaaaaa
    13741 aaccaggaaa ggagtatacc tgcgcggtgc ggcaaataca gccagcgcgt taactggaat
    13801 gcaattttgc ggggcacgac gaaaaaaaag cgcaaatctg gcatcggctt gccgcgagcg
    13861 gtcgtatatt ttgatcttca gaggctattt tatcgattca gctgtagtaa aattacgcaa
    13921 attttgactc ttgagtatga ggttgtcgca atgtcggttt ctaaaaaacc tatggtactg
    13981 gtgattctgg atggctatgg ctatcgtgaa gaacagcagg ataacgccat ttttagtgct
    14041 aaaaccccgg taatggatgc actgtgggcc aatcgtccgc ataccctgat cgacgcttcc
    14101 ggtctggaag tcggtctgcc tgaccgtcag atgggtaact ccgaagtagg ccacgttaac
    14161 ctgggtgccg gccgcatcgt gtatcaggac ctgactcgtc tggacgttga aatcaaagat
    14221 cgcgctttct ttgctaatcc agtgctgact ggcgcggtag ataaagcgaa aaacgcaggt
    14281 aaagcagtac acattatggg tctgctctcc gtaggcggcg tacacagcca cgaagatcac
    14341 atcatggcga tggtagaact ggcagctgaa cgcggcgcag aaaaaatcta cctgcacgca
    14401 ttccttgacg gtcgcgacac cccgccgcgc agtgctgaat cctcgctgaa aaaatttgaa
    14461 gagaaatttg ctgcgctggg taaaggccgc gtagcgtcca tcattggtcg ttactacgcg
    14521 atggaccgcg ataaccgttg ggatcgcgtt gaaaaagcct atgacctgct gactctggcg
    14581 cagggtgagt tccaggccga tactgccgtt gctggtttgc aggcagctta tgctcgcgac
    14641 gaaaacgacg agttcgtgaa agcgaccgtt attcgtgcag aaggtcagcc agatgcggca
    14701 atggaagacg gcgatgcgct gattttcatg aacttccgtg ctgaccgcgc gcgtgaaatc
    14761 actcgtgcat tcgtgaacgc tgatttcgat ggcttcgcgc gtaagaaagt ggttaacgtc
    14821 gatttcgtga tgctgaccga atacgccgct gacatcaaaa ctgcggttgc ttacccaccc
    14881 gcttccctgg ttaacacctt cggcgagtgg atggcgaaaa acgacaaaac tcagttgcgt
    14941 atttccgaaa ccgaaaaata tgcccacgtt actttcttct tcaacggtgg cgtagaagag
    15001 tcgttcaaag gcgaagatcg cattctgatc aactcaccga aagtggctac ctacgatctg
    15061 caaccggaaa tgagctccgc agagctgacc gaaaaactgg ttgcggccat caagagcggc
    15121 aaatacgaca ccattatctg taactatccg aacggcgaca tggtaggtca caccggggtg
    15181 atggaagcgg cagttaaagc ggttgaagca ctggatcact gcgtggaaga agtcgcgaaa
    15241 gcggttgagt ccgtgggtgg acaactgctg atcaccgctg accacggtaa cgctgaacag
    15301 atgcgcgatc cggcaacggg tcaggcacac acggcacaca ccaacctgcc agttccgctg
    15361 atttatgttg gtgataagaa cgtgaaagcg gttgaaggcg gcaaactttc tgacatcgcg
    15421 ccgaccatgt tgtcgctgat gggtatggaa atcccgcaag agatgactgg taagccgctg
    15481 ttcatcgtgg aataatccct ccccatgagg ggaaaggcga ttaataccat gacacgggcc
    15541 gtgaaaccgc gcaggtttgc aatcaggccc atcatctacg ccagcgttct tagcgctggc
    15601 gtattgttgt gcgccttttc cgcccacgcg gatgagcgtg accaactcaa atctattcag
    15661 gccgatatcg ccgcaaaaga gcgcgcggta cgccaaaagc aacaacaacg cgcaagcctg
    15721 ctcgcacaat tgaaaaagca ggaagaagcg atctctgaag ccacccgtaa gctgcgcgaa
    15781 acgcaaaaca cgctcaatca actcaataaa cagattgatg agatgaacgc gtcgattgcc
    15841 aaactggagc agcaaaaagc cgcccaggag cgcagcctcg ccgcgcaact ggatgccgca
    15901 ttccgtcagg gcgagcatac cggtattcag ctaattctca gcggtgaaga aagccagcgt
    15961 ggacagcgtt tacaggctta tttcggctat ctcaaccagg cgcgacaaga aaccattgcc
    16021 cagttgaagc aaacgcgtga agaagtcgcc atgcagcgtg ctgaactgga agagaaacaa
    16081 agcgagcaac aaacgctgtt atatgaacag cgcgcccaac aggcgaaact gactcaggcg
    16141 ctgaacgagc gtaaaaagac gctggcaggg ctggagtctt ccatccagca aggtcagcaa
    16201 cagttgagcg agctgcgcgc caacgaatcc cgtctgcgta acagcattgc ccgtgcggaa
    16261 gccgcggcga aagcgcgtgc agaacgagaa gcacgtgagg cccaggcggt tcgcgaccgc
    16321 cagaaagaag cgacgcgtaa aggcaccacc tacaaaccga ctgaaagcga aaaatcgctg
    16381 atgtcccgta ccggtggtct gggcgcgccg cgtggtcagg cattctggcc ggttcgcggg
    16441 ccgacgctgc atcgctatgg cgaacagcta cagggtgaat tacgctggaa agggatggtg
    16501 attggtgcct ctgaaggtac tgaagttaaa gcgattgccg acggtcgggt gattctggct
    16561 gactggctgc aaggctacgg tctggtggtg gtggttgagc atggtaaagg cgacatgagt
    16621 ctttacggct ataatcagag cgcactggtg agcgttggtt cgcaggttcg cgcgggccag
    16681 ccaattgcac tggtgggcag cagtggcggt cagggtcggc cttcactcta tttcgaaatt
    16741 cgccgccagg gtcaggcggt caatccacag ccgtggttgg gaagataagt tttgtttcca
    16801 tttcgtcgta acgttcttgc atttgccact ctgttggcgc tctcctcccc cgtacttgct
    16861 ggcaaacttg ccatcgtcat tgatgatttt gggtatcgcc cgcacaacga aaaccaggtg
    16921 ctggcgatgc cttccgctat ctccgtcgct gtattacccg attcaccgca cgccagagag
    16981 atggcgacca aagcgcataa cagcggtcac gaagtgttga ttcatctgcc aatggcaccg
    17041 ttgagtaaac agccgctgga gaaaaatacg ctgcgcccgg agatgagcag cgacgaaatt
    17101 gagcgcatta ttcgtagtgc ggtcaataac gtgccctatg ccgtggggat caacaaccac
    17161 atgggtagca agatgacctc taacctgttt ggtatgcaga aagtgatgca ggcgctggag
    17221 cgttacaatc tttacttcct cgacagtgta accattggta atacccaggc gatgcgcgcc
    17281 gcgcaaggca ctggcgtgaa ggttattaaa cggaaggtgt tcctcgacga ttcacagaac
    17341 gaagcggaca tccgtgtgca gttcaatcgc gcaattgatc tggcgcgtcg caacggttca
    17401 accattgcca ttgggcatcc tcacccttca acggtacgcg tgctacaaca gatggtttat
    17461 aacctgccgc cagacattac gctggtgaaa gccagcagct tgctgaatga accgcaggtt
    17521 gatacgtcta caccgccgaa aaacgctgtg cctgatgcac cgcgtaatcc gttccgtggc
    17581 gtgaagctgt gcaaaccgaa gaaaccgata gaacctgttt atgctaatcg cttctttgaa
    17641 gtattaagcg aaagcatcag ccagagcacg ctgatcgttt acttccagca tcagtggcaa
    17701 ggctggggca aacagcccga agcggcgaag tttaacgcta gcgcaaatta aaggcgctac
    17761 gcgtaatgcg atggtgcgac cgcgttttgt cgcgccattg ccataagcgg aaagaccaca
    17821 gtaacgcctg gtaacccacc ttcacgctgc gcacattggt aatcaggcgc ttatacatac
    17881 cggaagtaaa tatctcggca atcatccgtt ggcgggtaag aatatccggc tctttgcgca
    17941 ccgcatggca aacacgcaat gcttcgtaag ttatttgctg atgaaattcc ggataaatcg
    18001 taattttgtc ggcataattt cgatttaatt tctccagcag gcgggtaatc ttaatatagt
    18061 gacgttgata attaaggttt ttattccctt gtctatgcaa ccgactcact gacgtattat
    18121 gcagataata tttatataat gattgctcgg tatatcgcgc acgcagcgca ttaaacatga
    18181 attctgttgt ccagacaata tcctgatgat gtaatccggc aataaattta atgttatttt
    18241 taacaataac atcgcggcga taaaccccca tccagacaac gtgagtccaa cggcgcgaag
    18301 aaagccccat ccgcagccag tccgggccgg ttaatacacc ggttgagcga aggcgatcgc
    18361 tggggatgga ttgccaggtt tctcccgttt cacgaaaaca ccagtcagcg ttgcactgcg
    18421 ccacgtcgag gtcgtcctct aacgccatgg tcatcagcgt ttcgtacatg gtgggataga
    18481 cctcatcgtc agcatcgaca aaagcgacat atttgcccgt cgccacctcg atcccacgat
    18541 tacgcgctac cgatgcgcca gcattcgcct gatgcaacaa acgaacgtgc ggatagtttt
    18601 ctgcgtaatg ctttgctatt tcaacagaat tatccgttga accatcgtta ataataatga
    18661 tttccagagc agtccaggtt tgcgtaatta aagattccat acaagtgcgg aaatcatcgc
    18721 ccgcattata taacggaata ataacactaa gtttactggt gctgttcatc ataaattacc
    18781 gacgacctct gaagttatcc tgacgtttta caggctttgt attaagaaac tattaagcct
    18841 gtgagatatt atttttacag gtgagagagt cacttcaggg tagagagata atgagaacag
    18901 ctatataaga gatcagcgca attacgaact ctttattaca ctgacaggat tttacatatt
    18961 taacggatta tcctcaggca aaagggctgg aataccagcc cttgttcgtg ttaatcccag
    19021 ctcagaatta ctttcccgga ctggcccgaa cgcatagcgt caaagccctt ctggaaatca
    19081 tcaatagaga aacgatgggt aatgatcggc gagagatcga ggccagactg aatcagcgcc
    19141 gccatcttgt accaggtttc aaacatctca cgaccgtaaa tacctttaat gaacaagcct
    19201 ttaaagatca ctttggtcca gtcgatagac atatcagacg gcggaatacc cagcatcgca
    19261 atacggccgc cgtggttcat ggtgtcaagc atggtacgaa acgctggcgg cgcaccggac
    19321 atttccagac cgacatcaaa gccttcggtc atgcccagtt ctgccatcac atcattaagg
    19381 ttttctttcg cgacgttaac cgcacgggtg atacccattt tgcgcgccag ctcaaggcgg
    19441 tattcgttaa catcggtgat caccacattg cgtgcaccaa cgtgtttcgc caccgccgca
    19501 gccataatac ctatcggacc tgcaccagaa accagcacat cttcgcccac cagatcgaac
    19561 gacagcgcgg tatgcacggc gttaccgaag ggatcaaaaa tggaagccag gtcgtcggag
    19621 atattgtctg ggattttgaa ggcgttgaac gccgggatca ccagatattc ggcgaagcag
    19681 cccggacggt taacgccgac gccgatcgtg ttgcggcaca gatgggtacg accgccacga
    19741 cagttgcgac agtgaccgca ggtgatatgg ccttcgccag aaacgcgatc gccgattttg
    19801 aagcctttca cttcctgacc aatacctacc acttcaccga catattcatg gcctacgacc
    19861 atcggcaccg ggatggtttt ttgcgaccac tcatcccagt tatagatgtg aacgtcagtc
    19921 ccgcagatgg ctgttttacg gattttaatc agcagatcgt tatgcccgag ttccggtaca
    19981 ggaacgtcgg tcatccagat gccctcttcc gctttcagtt tggataacgc tttcatctca
    20041 catcctcagg cgataacgcc cagttgttta ccaatacgcg tgaatgcttc tactgcacgc
    20101 gtaatttgct caggggtatg cgccgcagac atctgggtac gaatacgcgc ctgacctttc
    20161 ggaacgaccg gatagaagaa accggtgacg taaatgccct ctttttgcag ctcacgggca
    20221 aatttctgcg ccactactgc atcgccaagc atgaccggaa taatggcatg atcggctccc
    20281 gccagggtaa agcccgccgc cgacatttgc tcacggaact ggcgcgcgtt cgcccacaga
    20341 cggtcacgca gttcgctgcc cgcttcgacc atctccagta ctttgatgga cgcggcaaca
    20401 atggccggtg ccagcgagtt ggagaacagg tacggacgag aacgctggcg cagccactca
    20461 accacctctt tgcgcgccgc ggtataacca ccagaagccc cgcccagcgc tttaccaagc
    20521 gtaccggtga taatatcgac ccggcccatc acatcgcagt attcatggga accacgacca
    20581 ttttcaccga caaaaccgac cgcgtgggag tcgtctacca tcaccagggc atcgtattta
    20641 tctgccagat cgcaaacgcc cttcagattg gcaatcacgc cgtccatcga gaatacacca
    20701 tcggtggcaa tcagcacatg acgcgcaccg gcttcacgcg cttctttcag acgagcttca
    20761 agctcctgca tatcgttgtt ggcatagcga tagcgtttag ctttacacag acgcacacca
    20821 tcaataatag acgcgtggtt cagtgcgtcg gagataatgg cgtcttccgc gcccagcagc
    20881 gtttcaaaca ggccaccgtt agcatcaaag caggaagagt agagaatcgc atcttccatc
    20941 cccaggaagg ccgccagttt ttgttcaagc tctttatggc tgtcctgagt gccgcaaata
    21001 aaacgcaccg aagccatgcc gaaaccgtga gaatccattc ccgcctttgc cgccgcaatc
    21061 agatcaggat gattcgccag cccgagatag ttgttggcac aaaagttaat gacgtggctt
    21121 ccatcagcca cagtgatatc tgcttgctgc gcagacgtaa taatgcgctc ttctttaaac
    21181 aacccttccg cccgtgcggt ttccagatcg ttggttaact gctgataaaa ttctccacgc
    21241 attgcgattc tccagacagg gcaaattcca gcacatatta cccaaactta taggtcgcga
    21301 cgagatgacg cgttaacact tctgcaaaat tcaggataaa taacgtgtaa cttgatgtct
    21361 ctcaacacct acagatagta gaaaaactgt acagttttgg ctgggcataa tgatgattga
    21421 ttttactgca ttgatataaa aagaaaaaat gatgccaaag catcattttt cctatctcag
    21481 ttggtatcaa aacgctaccc actaatggag ggaaacctat tttcttgaaa ggaaaattct
    21541 cagcgcctgc atttttgccc ctaaagtgtt actcctgaag cagcgaaaca aatcgaacca
    21601 ttttcctctc cccagatagt ttaggttgaa gagatcgggg cgtttgtaat agcacatgac
    21661 aaaaatgccc tggtcatcat ccacaatgtt attatttagt gttatttttt gactttccag
    21721 aactaattta tagaattctt tccatttatg ctgtgaacca acaattgcgc ccccaataat
    21781 atatacatga ttgccaatca tgaaatcaaa aacctgttgt tgagatgtga cagtaagccc
    21841 tttttttata gtgaaaagat gcatcttact ttcatcaaat gggaaatccc aaattttcaa
    21901 tcctcgagtt acatttggct tacggcaata gccaaaatct atccacgcaa caagaggcgt
    21961 tttcactaag cccatgttga tagctttatt tacaaaatag gccttaaggt tacataccaa
    22021 aacatactct ggtgaccagt actctgggtt ttttaattgt cgaggctcaa gtctatttgt
    22081 aaacgactca tctttttgaa ttttttcgat acgacttctg atatatctaa atttcttttt
    22141 tatatcgata acgataaccg ttgttggttt tccgttgcga atcgcttcaa cccttggttt
    22201 caaatcggga gaagtgaaaa taatcatctc attttcaagt gcggccagac gctcaaagta
    22261 actaaaataa acatcaactg aacgagctag tttttcacgg aaccctttat tagcagtcca
    22321 atcccctcta ccaatgtcaa aatatgcagt gataattgtg gtggat
//
LOCUS       DAZFXT010000074        22288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.074, whole genome shotgun sequence.
ACCESSION   DAZFXT010000074 DAZFXT010000000
VERSION     DAZFXT010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.074"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..714)
                     /locus_tag="ACHKW0_003051"
                     /pseudo
     CDS             complement(1..714)
                     /locus_tag="ACHKW0_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545887.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
     gene            complement(1373..1555)
                     /locus_tag="ACHKW0_003052"
     CDS             complement(1373..1555)
                     /locus_tag="ACHKW0_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475848.1"
                     /translation="MLLAENPRQGMTTEARVVIRILLISDHVNRQDNTQIQTIMRIIY
                     ICLLQKIKIIDKTKYK"
     gene            1494..3584
                     /gene="iha"
                     /locus_tag="ACHKW0_003053"
     CDS             1494..3584
                     /gene="iha"
                     /locus_tag="ACHKW0_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional siderophore receptor/adhesin Iha"
                     /protein_id="HFO7475849.1"
                     /translation="MRITTLASVVIPCLGFSASSIAAAEDVMIVSASGYEKKLTNAAA
                     SVSVISQEELQSSQYHDLAEALRSVEGVDVESGTGKTGGLEISIRGMPASYTLILIDG
                     VRQGGSSDVTPNGFSAMNTGFMPPLAAIERIEVIRGPMSTLYGSDAMGGVVNIITRKN
                     ADKWLSSVNAGLNLQESNKWGNSSQFNFWSSGPLVDDSVSLQVRGSTQQRQGSSVTSL
                     SDTAATRIPYPTESQNYNLGARLDWKASEQDVLWFDMDTTRQRYDNRDGQLGSLTGGY
                     DRTLRYERNKISAGYDHTFTFGTWKSYLNWNETENKGRELVRSVLKRDKWGLAGQPRE
                     LKESNLILNSLLLTPLGESHLVTVGGEFQSSSMKDGVVLASTGETFRQKSWSVFAEDE
                     WHLTDALALTAGSRYEHHEQFGGHFSPRAYLVWDVADAWTLKGGVTTGYKAPRMGQLH
                     KGISGVSGQGKTNLLGNPDLKPEESVSYEAGVYYDNPAGLNANVTGFMTDFSNKIVSY
                     SINDNTNSYVNSGKARLHGVEFAGTLPLWSEDVTLSLNYTWTRSEQRDGDNKGAPLSY
                     TPEHMVNAKLNWQITEEVASWLGARYRGKTPRFTQNYSSLSAVQKKVYDEKGEYLKAW
                     TVVDAGLSWKMTDALTLNAAVNNLLNKDYSDVSLYSAGKSTLYAGDYFQTGSSTTGYV
                     IPERNYWMSLNYQF"
     gene            complement(3802..3961)
                     /locus_tag="ACHKW0_003054"
                     /pseudo
     CDS             complement(3802..3961)
                     /locus_tag="ACHKW0_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066419.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(4164..4484)
                     /locus_tag="ACHKW0_003055"
                     /pseudo
     CDS             complement(4164..4484)
                     /locus_tag="ACHKW0_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053346.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
     gene            complement(4907..5482)
                     /locus_tag="ACHKW0_003056"
     CDS             complement(4907..5482)
                     /locus_tag="ACHKW0_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7475850.1"
                     /translation="MAVSLVKGGNVSLTKEAPTMNVAMVGLGWDARVTDGQGFDLDAS
                     VFAVGEDGKVLSDAHFIFFNNKTSPDGAVEHQGDNRTGEGDGDDEQVKIDLTKVSADI
                     KKLVFAVTIYDAEARKQNFGMVSNSFMRVYNNDNGTEIARFDLSEDASTETAMVFGEL
                     YRHGTEWKFKAVGQGFAGGLSALASQHGVNV"
     gene            complement(5551..6129)
                     /gene="terD"
                     /locus_tag="ACHKW0_003057"
     CDS             complement(5551..6129)
                     /gene="terD"
                     /locus_tag="ACHKW0_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerD"
                     /protein_id="HFO7475851.1"
                     /translation="MSVSLSKGGNVSLSKAAPSMKNVLVGLGWDARSTDGQDFDLDAS
                     AFLLASNGKVRGDSDFIFYNNLTSSDGSVTHTGDNRTGEGDGDDESLKIKLDAVPSEV
                     DKIIFVVTIHDAQARRQSFGQVSGAFIRLVNDDNQTEVARYDLTEDASTETAMLFGEL
                     YRHNGEWKFRAVGQGYAGGLASVCAQYGINAS"
     gene            complement(6178..7218)
                     /gene="terC"
                     /locus_tag="ACHKW0_003058"
     CDS             complement(6178..7218)
                     /gene="terC"
                     /locus_tag="ACHKW0_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance membrane protein TerC"
                     /protein_id="HFO7475852.1"
                     /translation="MVSTHIGFPTETVIVFIALSVGAIFIDLFMHRDDKPISLKSAAL
                     WSVFWVVVAMAFAGFLYIHHGAEVASLFVTGYALEKVLSVDNLFVMMAIFSWFAVPDR
                     YRHRVLYWGIIGAIVFRGIFVAIGTSLLSLGPYVEVVFAIIVAWTAVMMLKSGDDDDE
                     IEDYSQHLAYRMVKRFFPIWPKLRGHAFLLNQKEVDAELAKPENSDVTIGRGKKAALY
                     ATPLFLCVAVVELSDVMFAFDSVPAIIAVSREPLIVYSAMMFAILGLRTLYFVLEALK
                     QYLVHLEKAVIVLLFFIAAKLGLNATDHIWHHGYSIAATTSLYVVLGVLALGILASVM
                     FPGKPESEEKGS"
     gene            complement(7241..7696)
                     /locus_tag="ACHKW0_003059"
     CDS             complement(7241..7696)
                     /locus_tag="ACHKW0_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HFO7475853.1"
                     /translation="MSFFDKVKGAINSGRDELTRQVGRFKNKKFMQGTVAVCARIAVS
                     SDGVSSEEKQKMMGFLRSSEELKVFDTNEVIEFFNKLVSSFDFDVEIGKGETMKYILA
                     LKDQPEAAQLALRVGIAVAKSDGNFDQDEKLASREIAIALGFDPAEFGL"
     gene            complement(7719..8876)
                     /locus_tag="ACHKW0_003060"
     CDS             complement(7719..8876)
                     /locus_tag="ACHKW0_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309379.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7475854.1"
                     /translation="MNLTPGGNAPVPAQELRVRITSGGQVDASAFRLYADGKVQGDAD
                     MVFYGQPRNDDGTVSLVSEGQYSTFTVALNRLKPDVQKIAFTVTCDGGQTVSGLRNLS
                     IDVEQGATGLVSGSVELSGRQEAALILGEFYRRNNDWKFRFVAQGFNGGLKPLAEHFG
                     VNIADEPAPAAPTPPVVTPPPVETKISLSKVSLTKEKPAISLTKRDNFGEIRINLNWH
                     RGSSKSGFAGMFGSKGIDLDLGAFVELQDGYKSVIQALGNAFGDYRDEPYVQLKGDDR
                     TGDVSDGEWLHINGREWKHIREVLIYAFIYEGVPSWDKTDGVVTIHVPDQPPIETRLT
                     EGENRRTLCAIARLVNENGAIKVERINQYFKGQDEMDRAFGWGFRWSAGSK"
     gene            complement(8876..9457)
                     /locus_tag="ACHKW0_003061"
     CDS             complement(8876..9457)
                     /locus_tag="ACHKW0_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerD family protein"
                     /protein_id="HFO7475855.1"
                     /translation="MVSLVKNQTVSLSKESSALSQLHFGLGWDPVKKKGLLGGLFGGN
                     DSIDLDAGCVLMDSTGKTIDTIWFRKLKSTCGAVVHSGDNLTGEGDGDDEVIKVNLSR
                     LPANVEYLAFTVNSFRGQSFNDVENAFCRVVDQTGKELARYKLTEQGSHTGIVISSLR
                     RNNGNWDFTALGHACRGRTIDDMHSDIVSAVIR"
     gene            9780..10838
                     /locus_tag="ACHKW0_003062"
     CDS             9780..10838
                     /locus_tag="ACHKW0_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HFO7475856.1"
                     /translation="MNKKIWFMEGLSSQRDIIQGVKSFAQKNNFAITVFASHRNERNE
                     ILSVADYSLTEPEDPQKRLQFIQETIQTYGIHHIHTGRNSQWFEEHRSAIEPTGATLT
                     TGATGVDWLTLADEKVTFAQFMEQKGLPVVPSWRVNTLAELKTHLAAPPFTDSPVCVK
                     PVTGIYGMGFWRFDDSASPMAVFNHPEHRLVSPQQYIAAASAAESFKPLVLMPYLPGP
                     EFSVDILADKGEILAAVGRRKEGAIQYLVNEGSAWELACDCARVMKADGLVNVQTRND
                     VNGNPVLLETNMRPSGGVGYTLHSGVNLPGLFAAFKLGLMSEDMVRQSAKNTFSPVAV
                     RSITDVIAYPESLSNLLN"
     gene            10848..11990
                     /locus_tag="ACHKW0_003063"
     CDS             10848..11990
                     /locus_tag="ACHKW0_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase domain-containing
                     protein"
                     /protein_id="HFO7475857.1"
                     /translation="MSDILEDMIYRRTLSCGTIQVTRDQGEVSLDDLFDIAERRNPKR
                     AFLFVSKVLGRHIPVPPSVMRQAYRQLASQFPSTLTGPVLFIGMAETAVGLGAGVFDE
                     VRHQHRESVYLTSTRHPVDGTLLCEFKEEHSHATDHLIYLPDDEEKRRRVTNARTLVL
                     IDDEATTGNTFINLLSALRNTGKLQHIEQVIAVTLTDWSGNALSERTPLPVTSVSLVS
                     GQWGWTPLPDAPVPDMPKVNVTSRGEWDIQGKQSWGRLGMLAPAADLGHEVSVHKGER
                     ILVLGTGEFVWEPFLLAERLEAAGAQAFYGSTTRSPVAVGYAIESAISFTDNYGLGIP
                     NFVYNVAHQQFDRILVCTETPAESIDTQLLKALAEVAPVVEIVTYE"
     gene            11983..12756
                     /locus_tag="ACHKW0_003064"
     CDS             11983..12756
                     /locus_tag="ACHKW0_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475858.1"
                     /translation="MNKPVVLSDLDDTLFQTRRKMVDELALEPFRTGAVDRTLNPRSF
                     MTEEQSMLVDWLLEQAELIPVTARGTEEISRVRIPFHSWAITTHGAVILTPEGKPDEE
                     WKAHMLGQLAPYQEKLTSMQRLITEMMDAKGINAWARLNFEYGETAVYMVMKHRDSTR
                     LDELNAIADEIETVFPTEGFYIHRNSNNVAWLPTPVEKGLAVSWLLEKLRAERGVFPV
                     IGLGDSLSDHRFMKLCSWFGIPRQSQFADAISQRIFGEN"
     gene            12758..13837
                     /locus_tag="ACHKW0_003065"
     CDS             12758..13837
                     /locus_tag="ACHKW0_003065"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease StiP family protein"
                     /protein_id="HFO7475859.1"
                     /translation="MQPHETFTGSYQPGDVEFLLKPVVIEMTPVEKKEELIQSGKKHY
                     SDMLSQEPAPTQWHLDLFHRALDRGAERLAKEVTQLAIALAERFGDEPIVLASLVRAG
                     VPLGVMLHQALRDMGKTSWHYGISIIRDRGIDGAALDVIEERHGTSGIVFVDGWTGKG
                     AITGELVRALKDRPGYPEQPRLVVLADPCGCSWLAASDDDWLIPFGIMGAPVSGLISR
                     SVWSSEGLHGCMVCEHLSEFECSRMLADTVAHFRKKLTPSSLAPLSWNTESARVLWQT
                     SRDVIAFLADEFKVDSVNRIKPGIAEATRAVLRRVPDHVFVRSIDDPDVALLVGLARE
                     KGIVVTEMGGTLGQYRAVTIIKKVL"
     gene            13837..14793
                     /locus_tag="ACHKW0_003066"
     CDS             13837..14793
                     /locus_tag="ACHKW0_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HpcH/HpaI aldolase/citrate lyase family protein"
                     /protein_id="HFO7475860.1"
                     /translation="MKNRLSPWNLGATLYMPATREDIADAVLHGKIPGLRSLVICLED
                     AVSEADIPVALKNLEHLLHELSNSMSSLGKNDWPLVFIRPRHAEMGRWLKAHYDLSAV
                     DGFVLPKFTLSSLAEWWDIMVGTHLCMMPTLETEDVFDVVQMRELATRLEEHPCHDRI
                     IALRIGGNDLMNVVSLRRPRDLTLYDSPMGYVIKMLVSVFGPRDFALTAPVCEHIDDH
                     AVMARELALDMAHGLVGKTAIHPGQIEVIQNALMVTQGEHSDALRILNSTQAVFKSQG
                     AMCEPATHRRWAAGILDRARFYGLQNEQSADGIRLLTVTQHH"
     gene            14804..16012
                     /locus_tag="ACHKW0_003067"
     CDS             14804..16012
                     /locus_tag="ACHKW0_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4236 domain-containing protein"
                     /protein_id="HFO7475861.1"
                     /translation="MGFRFRKSINIIPGVRLNLSNGAPSLSVGPRGASVSFGSRGTYA
                     NLGLPGTGLSYRTRLDRAARSRGENRTATDPGLRQALEQKAAELMSAVTAIRNIHELT
                     PDPKTGISWAELEAVYLHNRTSPFQVPAPVRPEKPDYLVLPEKPAESEGISFLGKWFE
                     SESAKAERHAENLRRWQQELIDVERENTLRQHRYQQQRTAWAEQYANWKFEAEEHEKR
                     LATAQADARQQFRTDAAFFESYLAGVLAETEWPRETLVAFEVKPELSAVLLDVDLAEI
                     EDFPDKIYGVNARGTELTEKAMTQKAVRENYAHHVHGCLFRLVGIVLHTLPFDNVIVS
                     GFTQRVSKRTGYLEDEYILSCKCTRSQMSSVNFAGIKHIDPVEALGDQPVIRKMSSTF
                     IFQPIEPLTL"
     gene            16030..16497
                     /gene="terW"
                     /locus_tag="ACHKW0_003068"
     CDS             16030..16497
                     /gene="terW"
                     /locus_tag="ACHKW0_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurium resistance protein TerW"
                     /protein_id="HFO7475862.1"
                     /translation="MQLNTRQARIFKLANLLGTGKPVSAADIITSLECSEPTLTRALK
                     ELRESYSAEIKYSKAGHSYHLVNPGQLDKKTLRRMNEALAQNAELKTGESTGKVVLDK
                     DKKTAVSLSLRMRILRKIDRLAALSDSTRSEAVEKLALHSVDELIKEYSAKKS"
     gene            16758..17087
                     /locus_tag="ACHKW0_003069"
     CDS             16758..17087
                     /locus_tag="ACHKW0_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7475863.1"
                     /translation="MSKPRWTLDQKKHHVAAWRASGLTREQYCELYDIPFKSLRQWPQ
                     DVAKAEKRARAPEIIPVSVSGSSGMTDGRPLSDEPVTLFLPGGIRMCCQPSQLTDVFR
                     ALRHADA"
     gene            17074..17454
                     /gene="tnpB"
                     /locus_tag="ACHKW0_003070"
     CDS             17074..17454
                     /gene="tnpB"
                     /locus_tag="ACHKW0_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7475864.1"
                     /translation="MLTPDNVFLVVKPVDMRRGIDTLTQYVQNELNAAWHDGAAFVFT
                     NKVRSRIKVLRWDKHGVWLCTRRLHRGSFRWPRKGDATWHLTQDEFHWLVFGVDWQQV
                     KGHDLAKWVYQEEPEDAQSNAITQ"
     gene            17497..18327
                     /locus_tag="ACHKW0_003071"
                     /pseudo
     CDS             17497..18327
                     /locus_tag="ACHKW0_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(18358..19971)
                     /locus_tag="ACHKW0_003072"
     CDS             complement(18358..19971)
                     /locus_tag="ACHKW0_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element IS682 family transposase"
                     /protein_id="HFO7475865.1"
                     /translation="MSRKYLIRITELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEEEREIEHLRAQIEKLRRMLFGTRSEKLQREVEQAEAQLKQREQESDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGSELDYLGEVSAEQLE
                     LVSSALKVIRTVRVKKACTKCDCIVEAPAPSRPIERGIAGSGLLARVLTGKYCEHLPL
                     YRQSEIFARQGIELSRALLSNWVDACCQLMTLLNDTLYRYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGRIWTYVRDDRNAGSSEPPAVWFAYSPDRQGKHPVQHLRPFRGILQADAF
                     SGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEACSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(20002..20352)
                     /gene="tnpB"
                     /locus_tag="ACHKW0_003073"
     CDS             complement(20002..20352)
                     /gene="tnpB"
                     /locus_tag="ACHKW0_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7475866.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLDENPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            complement(20349..20774)
                     /locus_tag="ACHKW0_003074"
     CDS             complement(20349..20774)
                     /locus_tag="ACHKW0_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7475867.1"
                     /translation="MEQKILSSEPRRSFSNEFKLQMVKLASQPGASVARIAREHDIND
                     NLLFKWLRLWQNERRISRRLPVTTSSGAGVELLPVEITPDEQKEPMAALTPLLSTPSQ
                     STVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            20828..21579
                     /locus_tag="ACHKW0_003075"
                     /pseudo
     CDS             20828..21579
                     /locus_tag="ACHKW0_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         5534 a         5704 c         5646 g         5404 t
ORIGIN      
        1 tcatcttctt ccgccatcaa catctctgca tacttaaaca tttcagaacg ttcctttagc
       61 acagaagagt aattatatgt ccagttccca ggcaataatg cttatggata tttaattcat
      121 aatttagaga atattttgca ataatatttg gcagtattca gaaatacctg aaaaatcata
      181 ctatacagcc ctagggaatg gataaagatt ctaacaaagc atttcaacaa tatatacttg
      241 ttaaaaatcc atatcgaaat gccgttgcag cattaatata tgcctcattc ataaaatgta
      301 aaagagcaag ctcgtaccag taggggggaa attcattgac atgtcctgtc aatacgtaca
      361 gagccctgcc atgcttgcca gcacgtaaca attccgcccc cagtaaccag cgtgttccct
      421 gattattctc agggttatat gttagtattt tatcaatgag tataacggca tcctgatgac
      481 gctgtaaatg aacattcgcc agaattgcag cataaagagc gcaaatgcat atgccagatg
      541 agcgtgaata tcaatgatgt caggagcatg tcaggcaaga cgcctgagtt tgttgacgta
      601 atttgtttcg tttatttcat cactatcgta tgcgtcaaga gttttttcaa actcatcccg
      661 aaatgggcca agcctggttt caggaaataa gaaataccct tggttattgt tcacttaaaa
      721 gtataatttg taattcaaat cctgagcctg taaagcgggg agtaacatat tctattctga
      781 agagaataaa agtcgtgatg cgatgtatca agcccggatt gtaatcccag attaacatag
      841 atcacaaaac aacttattct tcactaacgt caagataaat atcgttgtat gccttatcac
      901 gactacgaat accagcaaga tacaactgac atacggaaag ataccacgtt ttttactccc
      961 gaaaataacg ctaaaaagct acttccccat cgtttgtcct tagtattgcc agcgccaaca
     1021 atgtgggctg acatgataaa gctgtctagg aaattgttcg cctcctcagc ggacaatcca
     1081 aatggtgatt gtctctgtta aacgtttatt ttgaaggtcg actgaataag gtgatgacgc
     1141 tgtagaattt ttcacgtgcc acagaatttt gaacgctttc tcttacaata tttcaatgtt
     1201 tctatcagta ttcgccggaa gaagtcatcg accaaatcat cccagtcgtc tcgcatcact
     1261 gaccattcat ggttgacatg tggtggaaat ccgcttctac agtaaccatt ttttattcgc
     1321 aaaaccgaac acgccatacg gatagctgtt aactggcatg ccgaggcagt cgttatttat
     1381 atttggtttt gtcaataatc tttatttttt gtaaaaggca aatataaatt attctcatta
     1441 ttgtttgtat ttgtgtattg tcttgccggt taacatgatc ggagattagt aatatgcgaa
     1501 taaccactct ggcttccgta gtcattccct gtctcggatt ttcagccagc agcatagctg
     1561 ctgcagagga tgtgatgatt gtctcggcat ccggctatga gaaaaagctg actaacgcag
     1621 ccgccagtgt ttctgtgatt agccaggagg aattgcagtc cagccagtac cacgatctgg
     1681 cggaggctct gagatcagta gagggtgtgg atgttgaaag tggtacgggt aaaaccggag
     1741 ggctggaaat cagcatccga ggaatgccag ccagttacac gctgatactg attgatggtg
     1801 ttcgtcaggg cggaagcagt gacgtgactc ccaacggttt ttctgccatg aataccgggt
     1861 tcatgccccc tctggccgcc attgagcgta ttgaggttat cagggggccg atgtccacac
     1921 tgtatggctc tgatgcgatg ggcggtgtgg tgaatatcat taccagaaag aatgcagaca
     1981 aatggctctc ttccgtcaat gcagggctga atctgcagga aagcaacaaa tggggtaaca
     2041 gcagccagtt taatttctgg agcagtggtc cccttgtgga tgattctgtc agcctgcagg
     2101 tacgcggtag cacacaacag cgtcagggtt catcggtcac atcactgagc gatacagcag
     2161 ccacgcgtat tccttatccc acggagtcac agaattataa tcttggtgca cgtcttgact
     2221 ggaaggcgtc ggagcaggat gtgctctggt ttgatatgga taccacccgg cagcgttatg
     2281 ataaccggga tgggcaactg gggagtctga cggggggata tgaccggacc ctgcgctatg
     2341 agcgaaacaa aatttcagct ggctatgatc atactttcac cttcggaaca tggaaatcgt
     2401 atctgaactg gaacgagaca gaaaataaag gtcgtgagct tgtacgcagt gtactgaagc
     2461 gcgacaaatg ggggcttgcc ggtcagccgc gggagcttaa ggaatcgaac cttatcctga
     2521 attcattact gcttacccct ctgggagaat ctcatctggt tacggtgggg ggcgagtttc
     2581 agagctcgtc catgaaagac ggagttgtcc ttgccagcac aggtgaaact ttccggcaga
     2641 aaagctggtc ggtatttgct gaggatgagt ggcatctcac ggatgcactt gcgctgactg
     2701 cgggcagccg ctatgaacat catgagcaat tcgggggaca cttcagtccg cgtgcatatc
     2761 tggtctggga tgtggcagat gcctggacgc tgaaaggcgg tgtgaccacg ggatataagg
     2821 cacccagaat ggggcagcta cataaaggga ttagtggtgt gtccgggcag ggaaaaacaa
     2881 atctacttgg taaccccgac ctgaagccgg aagagagcgt cagttatgag gctggggtgt
     2941 attacgataa ccccgccggt ctgaatgcca atgtcacagg ttttatgact gacttctcca
     3001 acaagattgt ctcttattcc ataaatgata acaccaatag ctatgtaaac agcggaaagg
     3061 cccggttgca cggtgtggaa tttgccggca cattgccgct gtggtcagag gatgtcacgc
     3121 tgtcactgaa ttacacctgg acccgaagtg aacaacgtga tggtgataac aaaggtgcgc
     3181 cgctgagtta tacccctgaa cacatggtga atgcgaaact gaactggcag atcaccgaag
     3241 aggtggcatc atggctgggt gcccgttatc gcgggaaaac accacgtttc acccagaatt
     3301 attcgtcact gagcgctgta cagaagaaag tgtatgatga gaaaggagaa tacctgaaag
     3361 cctggacggt ggtggatgca ggtctgtcgt ggaagatgac ggatgccctg acgctgaatg
     3421 ctgcggtgaa taacctgctc aacaaggatt acagtgacgt gagcctgtac agtgccggta
     3481 agagtacgct gtatgccggt gattacttcc agacgggatc atcaacaaca ggatatgtga
     3541 tacctgagcg aaattactgg atgtcgctga actatcagtt ctgataataa caaaacgcta
     3601 tcactgacgg tagaatacgt tgccactgca actcctggcg gaacagtggc aacgtattag
     3661 gttaagtgca tttccgatcc gctaatgaga tttcgttacc aacaactaat atcgtcacag
     3721 gaaatgcacg gattattttt aacttatcat ttacatactt gtccagagtg taagcgcacc
     3781 gtgaaggacg tggggtaaaa attagtttac agagagagtg acgttccagg gcaacaactc
     3841 tttcatgcgg ttggcaggcc aggtgttgat tacactgatc acgtggcgta gccacgtttc
     3901 cggctcgatt ccgttaagtt ttgcagctac cgatcaggct gtacatcact gccgcactat
     3961 cgctcgtcat ctcaaagtcc ggtctcgtca gcaggaaggt atcattctct cccgccattt
     4021 ttccaggggc ccggtcagat aagtcccttt gtctatcgct gactcctgac tcataacccg
     4081 gttagcagaa tgcagggtca ccacctcgcc acgaccaaat ccaaataagt caattgcacc
     4141 ttctcaatcg ccattttgtc agtaagcgta cagcctgaac tgatggtatc ttcaccatca
     4201 atgacaacgg tgatcgcaat tttactgacg ttcgccggaa cacgatccag tgctatctca
     4261 atgctggcct gctgcgaacc ggtaacgagc ctgactgccc cctcaggaga agacaaatta
     4321 ttataaaaga taaagtcaga atcgccactg acctttccct gagcattaag catgaacagg
     4381 caggtatcgg gttcgccttt aaagccggat ttggctgggt actgaagatt cagcctgatc
     4441 gtagattgct gaaggggtat gttttgtccg gattgtaaat tcatattaac tctcctgatt
     4501 tttgattatt attaatgcgc agcgtttata tatgttccct aggcttagtt ctggacgctg
     4561 gatattcggt gaggcgtaaa tatggtatga caccattttt cataacgctg aagtttctat
     4621 acctgttgaa tttgaatttt cattgaccgg gtatcttatt ttccagggcc cgttccttca
     4681 taagtcgcaa aagtaacata tatccgaagg gcatgctgtt gatatcagac acggaatact
     4741 ggctttaacc agcaaccaca gataaacccg gggcctgcat aaaaggttca tgccagaatt
     4801 aacatagctc tgctttctgc cccccccctt tcccatattc acccgttgat agcggatcga
     4861 tacccaaaaa aaaccccggc attgccgggg tcagactcag cgtcttttag acattgacgc
     4921 cgtgctggga agcaagcgct gacagaccac cggcaaaacc ctggccaaca gctttaaact
     4981 tccactcagt accatggcga tacagttcac cgaagaccat tgcggtttcg gttgaggcgt
     5041 cttcagacag atcgaaacgg gcaatttccg tcccgttgtc gttgttgtaa acgcgcatga
     5101 agctgttgct caccatgccg aagttttgtt tacgcgcttc tgcatcatag atggtaacgg
     5161 caaataccag ttttttgatg tctgctgaga ctttggtcag atcgattttg acctgctcat
     5221 cgtcgccgtc gccttcaccg gtacggttgt cgccctggtg ctctactgcg ccatcagggc
     5281 tggttttatt attgaagaaa atgaaatggg catctgacag tactttaccg tcttcaccta
     5341 ctgcgaatac ggaagcgtcc agatcaaaac cctgaccatc ggttacacgg gcatcccagc
     5401 ccaggccaac catagcgaca ttcatggttg gtgcttcttt ggtcagagat acgttgccgc
     5461 cttttacgag agaaactgcc atttttagct cctgcaaaca gttgaatgag gctgaattaa
     5521 cagccccaga tatgaaaagt tacttttcga tcaggacgcg ttaatgccgt actgagcaca
     5581 tacagatgcc agaccaccag cataaccctg gcctactgcg cggaatttcc actcaccatt
     5641 gtggcgatac agctcgccga acagcatggc agtctcagtg gacgcatctt cggtcagatc
     5701 gtagcgagcg acttcagtct ggttatcgtc attaaccaga cgaataaacg caccggatac
     5761 ctgaccaaag ctctggcgac gagcctgagc atcgtggatg gtcacaacga agatgatctt
     5821 gtcaacttca gacgggacgg cgtccagttt aattttcagc gattcatcat caccatcgcc
     5881 ctcaccggtg cggttatcgc cggtgtgcgt tacggaaccg tcggatgacg tcaggttgtt
     5941 atagaagatg aaatctgaat cgccgcgcac tttgccgttt gaggccagca ggaatgctga
     6001 agcatccagg tcaaagtcct gaccgtctgt tgaacgcgca tcccagccaa ggcccaccag
     6061 gacatttttc attgacggag ctgctttact cagggagacg ttcccgcctt tggaaagaga
     6121 aacactcata aaataacctc ttcgattagt aattgttcag gttaacactt aaggggatta
     6181 actccccttt tcctcagatt caggtttgcc cgggaacatg acgcttgcga gaatgcccag
     6241 cgccagtaca cccagtacaa catacaggct ggttgttgcc gcgatgctgt aaccatgatg
     6301 ccagatgtga tcggtcgcat tcaggccgag ttttgccgcg atgaagaaca gcagcacgat
     6361 aacggccttc tccagatgaa ccaggtactg tttcagtgcc tcaaggacaa aatacagagt
     6421 acgcaggccc aggatagcaa acatcatggc actatagacg ataagcggtt cacgactgac
     6481 ggcaatgatt gccggtaccg agtcaaacgc gaacattacg tccgagagtt caaccacagc
     6541 cacacacagg aacagcgggg tcgcatacag cgccgctttt ttaccacggc caatggtgac
     6601 atcgctgttt tctggtttcg ccagttcagc atccacttcc ttctggttaa gtaggaaggc
     6661 atgccctctg agcttcggcc agatagggaa gaagcgttta accatacggt aagccagatg
     6721 ctgggagtaa tcctcaattt catcatcgtc atcaccgctt ttaagcatca tgaccgccgt
     6781 ccaggcaacg ataatagcga agacaacttc aacgtacggc cccagactca gcaggctcgt
     6841 accgatggcg acaaagatgc ccctgaagac gatggcacca atgatccccc agtagagaac
     6901 acggtggcga taacgatccg gaacggcgaa ccaggagaag attgccatca tgacgaacag
     6961 gttatcgacc gacagcactt tctccagcgc ataacccgtg acaaacagac tggcaacctc
     7021 agcaccgtgg tggatataga ggaaaccggc aaatgccatc gcaaccacaa cccagaatac
     7081 ggaccagagc gcagcactct tcagcgagat tggcttgtca tcacggtgca taaacaggtc
     7141 aataaagatg gcaccgactg aaagcgcaat aaaaacaatg accgtttcag tcgggaagcc
     7201 gatgtgtgtg gaaaccatat tttaccctta gggatgtgga tcagaggcca aattcagccg
     7261 ggtcgaagcc caacgcgata gcgatctcgc gggaggccag tttctcgtcc tgatcgaagt
     7321 taccgtcact tttcgcaacg gcaataccaa cacgtaaggc cagctgagcg gcctcaggct
     7381 gatctttcag cgccaggatg tatttcatgg tttcgccctt gccaatttca acatcgaaat
     7441 cgaagcttga aaccagttta ttgaagaact caatcacctc attggtatcg aagaccttca
     7501 gctcttctga agagcgcaga aagcccatca ttttctgctt ttcttccgaa cttacgccgt
     7561 cactcgatac ggcaatacgg gcacatacag caacggtgcc ctgcatgaat tttttgtttt
     7621 tgaaacggcc aacctggcgg gtcagttcgt cacggcctga gttaatggca cctttaactt
     7681 tgtcgaaaaa gctcatacct gtttcctttg ttgctgtgtt atttagaacc ggcgctccag
     7741 cgaaatcccc agccaaatgc ccggtccatt tcgtcctggc ctttgaagta ctggttaatt
     7801 cgctcgactt taattgcgcc gttttcgttt acgagtctgg caatggcgca caatgtgcga
     7861 cggttctcac cttcggtcag gcgggtctca atgggcggtt gatccggcac atgaatggtg
     7921 actacaccat cagtcttgtc ccagctggga acaccttcat aaataaaggc gtaaatcagc
     7981 acttcgcgga tatgcttcca ttcacgtcca ttgatatgca gccactcgcc gtctgataca
     8041 tcacccgtcc ggtcatcgcc cttgagctga acatacggtt catcgcgata atcaccgaat
     8101 gcattaccga gcgcctgaat gaccgattta tacccgtcct gaagctcaac aaaggccccc
     8161 agatccaggt cgattccttt ggagccgaac atacctgcaa agcctgattt gctactgccc
     8221 cggtgccagt tcaggttgat ccggatttca ccgaagttat cccgtttggt caggctgatt
     8281 gcaggcttct cttttgtcag cgataccttg ctcaggctga tttttgtctc aactggcgga
     8341 ggggttacaa ccggaggagt tggggctgcg ggagccggtt cgtcggcaat atttacgccg
     8401 aaatgctctg ccagcggttt aagtccaccg ttgaacccct gcgcaacgaa gcggaatttc
     8461 cagtcattat tacgacggta aaattccccc agaattaagg ctgcttcctg acggccgctc
     8521 agttcgacac tgccgctaac caggccggta gccccttgct ccacgtcgat ggaaagatta
     8581 cgaagaccgg acaccgtctg cccaccgtca caggtcacgg tgaacgcaat tttctggaca
     8641 tcaggtttca gccggttgag cgcgacagta aaggttgagt actggccttc actgaccagg
     8701 ctgacggtac catcatcgtt acgtggctga ccataaaaca ccatgtccgc atcaccctgc
     8761 accttcccgt cggcatacag gcgaaacgcc gaagcatcca cctggccgcc cgaggtgatc
     8821 cggacccgca actcctgggc tggaacgggg gcattgcccc ccggcgtcag gttcattagc
     8881 gaataaccgc tgaaacgata tcggagtgca tatcgtcaat tgtgcggccg cgacaggcat
     8941 ggccaagggc ggtaaagtcc cagttgccgt tattacggcg cagcgaagaa ataacgatcc
     9001 cggtatgtga gccctgctca gtcagcttgt agcgagccag ctctttgccg gtttgatcga
     9061 caacgcggca gaaagcgttt tcgacgtcat tgaacgactg accacggaag ctgtttaccg
     9121 taaacgccag gtattcaacg tttgcaggca ggcgggagag gttgacctta atcacttcat
     9181 cgtcaccgtc accctcaccg gtcaggttat cgccactgtg cacaacagcg ccgcaggtgg
     9241 atttcagttt gcggaaccag atggtgtcaa ttgttttacc tgtgctgtcc attaatacac
     9301 agccagcatc gagatcgatt gagtcgttgc ctccaaacaa tcctccaaga agaccttttt
     9361 tcttaaccgg atcccaaccg agaccgaaat gaagctggct taatgcagat gattctttgc
     9421 tgagtgatac cgtctggttc ttaactaatg aaaccatgtt ttattcctta aaatggtaat
     9481 gaccgagaca atcaaaggta tatttagtaa ctgacgaaga aaatcatatc atgatgaagt
     9541 tctctgacaa gggaaatttt tgtggttttc tgtaaaaaca caaaaagaaa tccttttcag
     9601 ggtgttagtt tttctatttt taatttaaat atcaatgtgt tataaggaat ccaaaaaaca
     9661 ctgacgcact ccataaccac aggagttatt aatcatatta tgattgacat gtagcttaga
     9721 cactcataga ctcacttctc aatcataaat ttgcatggcg ttccggataa ttaaaaaaaa
     9781 tgaacaagaa aatttggttt atggaaggtt tatcctccca gcgagatatt attcaggggg
     9841 taaaatcatt tgcacaaaaa aataattttg ccattaccgt ttttgcctcc caccgtaacg
     9901 aaagaaatga aatcctttcc gttgccgatt attctttgac tgaacctgaa gatcctcaaa
     9961 aacgtcttca gtttatccag gaaaccattc agacctacgg catccaccat attcatactg
    10021 gccgtaacag ccagtggttt gaagaacacc gttcagccat tgaaccgacc ggtgccaccc
    10081 ttactaccgg tgcaacgggc gtcgactggt taactctggc tgacgaaaaa gttacttttg
    10141 ctcagtttat ggagcaaaag ggtctcccgg tcgtaccatc ctggcgggtg aatacgctgg
    10201 cagaattaaa gacacacctc gcggccccgc cgttcactga cagcccggta tgcgtgaagc
    10261 cggtgacggg tatctatggc atgggattct ggcgctttga tgacagtgct tcgcctatgg
    10321 ccgtctttaa tcatcccgaa catcgtctgg tcagtccgca acagtatatt gcagcagcat
    10381 cagctgctga gtcgtttaaa ccccttgttt tgatgccgta cctgccaggc ccggaatttt
    10441 ccgtcgatat cctcgcggat aagggcgaaa tactcgcagc cgtgggacgc cgtaaggaag
    10501 gggctatcca gtatctggta aacgaaggaa gcgcctggga actggcgtgt gactgcgccc
    10561 gtgttatgaa ggccgacggg ctggtgaatg ttcagacgcg aaacgatgtg aatggcaacc
    10621 cggtgctgct tgaaaccaac atgcgtccgt cagggggggt gggttatacc cttcacagcg
    10681 gtgtgaacct tcctgggtta tttgctgcct ttaagctcgg tctgatgtct gaagatatgg
    10741 tacgccagag cgctaaaaac accttttctc cggttgcggt gagatccatt acggatgtaa
    10801 ttgcataccc ggaatcactc tctaaccttc tgaattaagg cgtatttatg tctgatatcc
    10861 tcgaagatat gatttatcgc cgtaccctct catgcggtac gattcaggta acccgtgacc
    10921 agggtgaagt ttcgctcgat gacctgtttg acatcgctga aagacgtaac ccgaaacgcg
    10981 cctttctgtt tgtcagcaaa gtactcggca ggcacattcc cgtgccaccc tcagtcatgc
    11041 gacaggccta cagacagctt gccagccagt tcccctcgac actgacagga cccgtactgt
    11101 ttatcggtat ggctgaaacc gccgttgggc ttggggccgg tgtatttgat gaagtgcgcc
    11161 accagcatcg tgaatctgtc tatctgacct ctacccgtca cccggttgat ggcacgttgc
    11221 tttgcgagtt caaggaagaa cacagccacg ccaccgatca tctgatctat ctgccagatg
    11281 atgaagagaa aagacgtcgt gttaccaacg cacgaacgct ggttttgatt gatgacgagg
    11341 caaccaccgg taataccttt attaacctgc tttctgccct gcgtaatacc ggcaagcttc
    11401 aacatattga acaggttatc gccgtaacgc tgaccgactg gagtggcaat gccctgtcgg
    11461 agcgcacccc cttgcccgtc acttcggttt cgcttgtaag cggtcagtgg ggatggaccc
    11521 cattacctga tgcacctgtc ccggacatgc cgaaagtcaa cgtaacttca cgaggagaat
    11581 gggacatcca ggggaaacag tcatggggcc gactggggat gctcgcacct gcggccgatc
    11641 tcggccatga ggtctccgtc cacaaggggg aacgtattct ggttctcggg accggggaat
    11701 tcgtctggga gccgttcctg cttgctgaac ggctcgaagc tgccggagca caggcatttt
    11761 atggatcgac cacccgctcc cctgtcgccg ttggttatgc cattgagtcc gccatttcct
    11821 ttacggataa ctacgggctg ggcatcccca attttgtcta taacgtcgcc caccagcagt
    11881 ttgaccgcat tcttgtgtgt actgagacac ccgcagaaag tattgacacg cagcttctta
    11941 aagcgctggc tgaggttgcg cccgtcgtgg agattgttac ctatgaataa acccgttgtt
    12001 ctgagcgacc ttgacgacac gctctttcag acccgtcgta aaatggtgga cgagctggcc
    12061 ctggagccat tccgtaccgg tgccgttgat cgcaccctga atccacgaag ctttatgacg
    12121 gaagaacagt ccatgttggt ggactggctc ctggagcagg ctgaactcat acccgttacc
    12181 gcacggggaa ctgaagaaat cagccgcgta cggatccctt tccactcctg ggcaatcaca
    12241 acgcacgggg ccgtcattct cacgcctgaa ggcaaacccg acgaggagtg gaaggcccat
    12301 atgctcggcc agctggctcc ctaccaggaa aagctgacat cgatgcagcg tctgatcact
    12361 gaaatgatgg acgcaaaagg aatcaatgcc tgggcaaggc tgaacttcga atacggtgaa
    12421 acggcggttt atatggtgat gaaacaccgc gacagcaccc gccttgacga gctcaatgcc
    12481 attgcagatg agatagaaac ggtgtttccg accgagggct tctacatcca ccgcaacagt
    12541 aataacgtgg cctggcttcc caccccggtt gagaaagggc tggcagtcag ctggctgctt
    12601 gaaaaacttc gggctgaacg aggagttttc cccgtaattg gtttgggcga cagcctgagc
    12661 gatcatcgtt ttatgaaact gtgcagctgg tttggcatcc cgcgtcagag tcagtttgca
    12721 gatgccattt cacagcgaat ttttggagaa aattaaaatg cagccacatg aaacatttac
    12781 cggctcatac cagcccggtg acgtggaatt tctgctaaag ccggttgtca ttgagatgac
    12841 gccggttgag aaaaaagaag aactgattca gtccgggaag aaacattatt cggacatgct
    12901 cagccaggag ccagcgccta cacaatggca tcttgatttg tttcaccggg cgctggatcg
    12961 gggcgcagag aggcttgcaa aagaagtcac acagctcgct attgctctgg ccgaacgctt
    13021 cggtgatgag cccattgtac tggccagtct tgtcagggct ggcgttccgc tcggcgttat
    13081 gctgcaccag gccctgcgtg acatggggaa aacctcatgg cattacggta tcagcattat
    13141 ccgggatcgt ggaattgacg gtgcagccct cgacgtcatt gaagagcggc atggtaccag
    13201 cggtattgtc tttgttgacg gatggacagg taaaggcgca attaccggag agcttgtacg
    13261 cgcactgaaa gatcgcccgg gctatcctga gcagccgcga ctggttgtcc tggccgatcc
    13321 ctgtggctgc tcctggcttg cagccagtga tgatgactgg ctcattcctt ttggcatcat
    13381 gggagcgccg gtatcaggcc tgatctcccg gtcagtatgg tcctctgaag gattacacgg
    13441 gtgcatggtt tgcgagcatc tcagtgaatt cgaatgcagc cggatgcttg ccgataccgt
    13501 cgctcatttc cgtaagaagt taacaccgtc atccctcgct cccctgagct ggaatacgga
    13561 atcagcccgg gtcttatggc agacaagtcg cgacgttatc gcgttcctgg ccgatgaatt
    13621 taaagtggac agcgtcaatc gtattaaacc cggtattgct gaagcaaccc gggctgtatt
    13681 acgtcgggta ccggaccatg tatttgtgcg ttctattgac gacccggacg ttgccttgct
    13741 tgtagggctt gctcgtgaaa agggaatagt tgttacagaa atggggggaa ccctcggcca
    13801 gtatcgggct gtaaccatta tcaagaaggt actctgatga aaaatcgtct ttctccctgg
    13861 aatctgggag ccacgctata catgcctgca acacgggaag atattgctga tgccgtcctg
    13921 cacgggaaaa tcccgggctt gcgctctctg gtgatttgcc tggaggatgc tgtcagtgaa
    13981 gcagacatcc ccgtcgccct caaaaatctg gagcacctgc tgcacgagct cagtaacagc
    14041 atgagtagcc tgggtaaaaa tgactggcct ctggtgttca ttcgcccccg gcatgccgaa
    14101 atgggcagat ggttaaaagc gcattatgat ctttccgctg tcgacgggtt tgtgctgcct
    14161 aaatttaccc tcagttcgtt agctgaatgg tgggatatca tggtcgggac tcacctgtgc
    14221 atgatgccaa cgctggaaac agaagatgtc tttgacgtgg ttcagatgcg cgagctggct
    14281 acccgtctgg aggaacatcc ctgccacgac cgcattattg cgcttcgaat cggcggcaac
    14341 gatcttatga acgttgtgtc gcttcgccgt ccccgggacc tgacgctgta tgacagcccg
    14401 atgggctacg tcatcaaaat gctcgtttcg gtcttcggcc cgcgtgattt tgccctgacc
    14461 gcgcctgtat gtgagcatat tgacgatcat gccgttatgg ccagagagct ggctcttgat
    14521 atggcacacg ggcttgttgg gaaaacggcc attcacccgg gtcagataga ggtcattcaa
    14581 aacgcgctga tggtcactca gggtgagcat tctgacgctc tgcggatcct gaactctacc
    14641 caggccgtgt ttaagtcgca gggagcaatg tgtgaacccg ccacacatcg ccgctgggcg
    14701 gctggcattc tggacagggc tcgtttttat gggttacaga acgagcaaag cgctgatgga
    14761 atcagattac ttaccgtgac ccagcatcat taaggaaatc ataatggggt tcaggttcag
    14821 aaagtcgatc aacattattc ctggcgttcg cctcaacctg agtaacggtg caccgagcct
    14881 gagtgtcggg ccgagaggtg cttccgtttc ttttggtagc cgggggacct atgccaatct
    14941 gggcttgccc ggtaccgggc tgagttaccg tacccggctt gaccgggccg cgcgttccag
    15001 aggtgaaaac cggacggcaa ccgacccggg gctcagacag gcgcttgagc agaaagccgc
    15061 tgaactcatg tcagcggtaa ccgcaatccg taatattcac gagctgacgc cggatccaaa
    15121 aacaggcatc agctgggcag agctggaagc agtatacctg cataacagaa cgtcgccttt
    15181 tcaggttccg gcaccggtgc gtccagaaaa gccagactac cttgtattgc cggaaaagcc
    15241 tgccgagagc gaaggcatta gttttttggg taaatggttt gaatcggaat cagctaaagc
    15301 tgagcgccac gccgaaaatc ttcgccggtg gcagcaggag ctgattgatg tggagcgtga
    15361 gaataccctt cgacagcacc ggtaccagca acaacggacg gcctgggccg aacagtatgc
    15421 aaactggaag tttgaagctg aagaacatga aaaacggctc gccacggctc aggcagatgc
    15481 ccggcagcag ttccggacag acgccgcgtt tttcgaatca tacctggcgg gtgtgctggc
    15541 agaaactgaa tggccgcgtg aaacgcttgt tgcatttgaa gtaaagccgg agctatcagc
    15601 agtcctgctg gacgttgatt tagctgagat tgaagatttc cctgataaga tttacggcgt
    15661 taatgcccgg ggaacggagc tgacggaaaa agccatgacg caaaaagccg tacgcgaaaa
    15721 ctatgcccac cacgtccatg gctgcttgtt ccgcctggtc ggtatcgttt tacatacgct
    15781 acctttcgac aacgtgattg tgtcaggctt tacgcaacgg gtcagtaagc ggaccggcta
    15841 tctggaggat gagtatatcc tgtcctgcaa atgcactcgc agccagatgt cgtcagtaaa
    15901 ttttgcaggc ataaaacaca ttgatccggt tgaagcgtta ggcgatcaac cggttattcg
    15961 aaagatgagc agtaccttca tttttcagcc tattgaacca ctaacccttt aagcaggcta
    16021 aatcttacga tgcaattaaa taccagacag gcccggattt ttaagcttgc caacttactg
    16081 ggcaccggca agcccgtttc cgctgccgac attataacca gtctggaatg ctctgagccg
    16141 acattaaccc gggccctcaa agagctacgc gagtcatatt ctgcggagat caaatacagc
    16201 aaggcaggtc attcctatca tctcgttaat ccgggccagt tggataaaaa gaccctccgc
    16261 cggatgaatg aagcgctcgc acagaatgct gaactgaaaa ccggtgagtc cacagggaag
    16321 gtcgtactcg ataaggataa aaaaacagcc gtgtcattat cgctacgcat gcggattcta
    16381 agaaaaattg accgtctggc tgcgctgagt gactcgaccc gaagtgaagc ggtagagaag
    16441 ctggctctgc actccgttga tgaactgata aaagaataca gcgctaaaaa gtcctgacgc
    16501 taaatctcag accggggtga aaactccggt tttgtcatat ttcacctctg tattggcgtg
    16561 atgcaaaatg gcatctgaat cgatcatggt ttggcatcca tttccgatca cagtttggca
    16621 tcacaatcga tcacgatttg gcatgcttcc gctcattgat tggcatcctg gtgtcgttac
    16681 ggggaagtca tcttcacagg gatcttcagt cagttagcat taccgacatc cattcaccaa
    16741 cttaccggag ataatcaatg tcaaaacccc gatggacgct tgatcagaaa aaacatcacg
    16801 ttgccgcatg gcgcgccagc ggccttaccc gtgaacagta ctgtgaactt tacgacattc
    16861 cgtttaaatc cctgcgccag tggccgcagg atgtcgcaaa agccgaaaaa cgagccagag
    16921 caccagaaat tattcctgtt tcagtatctg gctcttccgg aatgactgat ggcagaccat
    16981 tgtcggatga gcctgtcact ctcttcctgc ccggtggtat ccggatgtgc tgccagcctt
    17041 ctcagttaac cgatgttttc cgggctcttc gccatgctga cgcctgataa cgtattcctg
    17101 gtcgtaaaac ccgtcgatat gcgccggggt atcgacacac tgacacagta cgtccagaac
    17161 gaacttaacg ctgcatggca tgacggtgct gcttttgtct tcaccaataa agtccgctcc
    17221 cgcataaaag ttctccggtg ggacaaacat ggcgtatggc tgtgtacccg acgactgcac
    17281 cggggaagct tccgctggcc ccggaaaggt gatgccacat ggcatctcac acaggatgag
    17341 tttcactggc tggtctttgg ggttgactgg caacaggtga aagggcatga cctggcaaaa
    17401 tgggtatacc aggaagaacc cgaagatgct cagagcaacg caataactca atgaaatata
    17461 atgaatatcc atgcgtgatg tcatataatc caccgcatgg atatctcgct tctcagtacc
    17521 accagtgacc ctgaacagct tcgcgctctg gcaattgcca tggtgcaaaa agtcatggct
    17581 gagaatgcag aacttcagaa ccgtatccgc atcctggaag agcagatgaa gctcgcacgc
    17641 cagcagcgtt tcggtaaaaa atgcgaatcg ctggccggga tgcagcgttc cctgttcgaa
    17701 gaggatgttg acgcggatat cgcagaaatc tcggcgcatc tggacaaact gcttcctcag
    17761 accggggacg aagaaaaaac aacaacacgt cctgttcgta agcccttgcc ttcacctctt
    17821 ccccgtgctg aaaaggttat cccgccagct gaggaacgtt gtcctgactg tgatgccccc
    17881 ctgcacttca ttcgcgatga agtcagcgag aagctggaat atattccggc acaggtcgtg
    17941 gtcaaccgct atatccgccc gcagtacagc tgtccctgct gtgagaaagt gttcagcgga
    18001 aaaatgccgg cacatatcct tccgaaaagt gctgttgagc catcggtcat cgcacaggtt
    18061 gtcatcagca aatataccga ccatctgccc ctgtatcgcc agcagcacat tttctcccga
    18121 atgggggtgg agctccccgt cagtacaatg gcggatatgg tcggtgttgc tggcgctgca
    18181 ctggccccgc tggcaaaatt gttacgccac gagcttctga cacgggatgt tattcatgca
    18241 gatgaaacgt ccctgaggct ccttgatacc cggaaaggcg gtaaatcctg ctccggctgg
    18301 ctgtgggctt acgtcagcgg agaaagagta agcgtcaacg gagcaccgta ttgacgctta
    18361 tttattggtg agtactacgt tccatggcag gagttcatca actcggttgg aaggccattc
    18421 tggcagtacg ctcaggatat ggcgcagata cgcttccgga tcgataccgt tcagacggca
    18481 ggtgccgatc agcccgtaca acagtgctcc acgctcgccg ccgtgatcgc tgccaaagaa
    18541 cataaagttt ttctttccga gacagactgc acgaagcgct ctttccgcag cattattatc
    18601 cgcctccgcc agaccgtcat cactgtaata acagagggca tcccactgat tcagtacata
    18661 gctgcacgct tcgcccagtc tggatttttt cgacagcgta ccattcttct ccaccatcca
    18721 ttcatgcagt gacgtcagta acactttgct tcgctgctgc ctgacggcaa gacgctctga
    18781 ctccggtaat ccccgtattt catcctcgat ggcgtacagt tcactgattc gcttcagggc
    18841 ttcttctgcc gtcgcacttt tgctgctgat gtatacatcg tggatttttc gccgggcatg
    18901 ggcccagcac gcaacttctg tcagtgcacc accttcacgt tctgcactga acagccgatc
    18961 gtaaccgctg aacgcatccg cctgcaggat accccggaag ggacgaaggt gttgtaccgg
    19021 atgttttccc tgcctgtctg gtgagtaggc gaaccagacc gccggtggct ctgatgagcc
    19081 cgcattccgg tcatcccgga catacgtcca gatgcgtcct gtttttgcct tttttctgcc
    19141 cggtgccagc acttttactg gtgtgtcgtc agtgtgaacc ttgcgggtgt tcatcacgta
    19201 acggtacagg gtatcattca gcagagtcat taactgacaa catgcgtcaa cccagttgga
    19261 gagtaatgcc cggctcagtt cgatgccctg gcgggcaaag atttcactct gacgatacag
    19321 tggcaggtgt tcgcagtatt ttcccgttaa cacgcgggca agtaatccgg agcccgcgat
    19381 gccgcgctct atcgggcggg acggcgctgg cgcttcaact atacagtcac attttgtaca
    19441 ggcttttttt acccgaacag tgcggatcac tttcagggcg ctactcacca gttccagctg
    19501 ctcagcactg acttccccca gataatccag ctcactgcca cactccgggc aacaactttc
    19561 ttcaggctcc agacggtgta tttcacgggg aagatgtgcc ggtaacggac gacgatgacg
    19621 tgactgtcgc aactggcggg gaacctgcgg gtcatcctca cgcccactgt aacgatcgct
    19681 ttcctgttcg cgttgtttca gttgggcctc agcctgttca acctcacgct gcagtttttc
    19741 agaacgggta ccgaacaaca tccggcgcag tttttctatc tgggccctca gatgttctat
    19801 ttcccgctcc tcctcttcga tcttttcctc ggcacgtgcc agtgcagagc gcaggaaggc
    19861 ctccgtctct tcaaccagac tcagttgctg atctttctga cggagggctt cagcctgctc
    19921 agagagcagc ctttccagct cagtgatacg aatgaggtat ttccgactca tgaccgtttt
    19981 tataatccgg ccatgacatt tttacaacat tgtcagtgca ttaaggcggg atgttttggg
    20041 ctgacgccag tccagtttat cgaggagcat tgccagctgc gagcgggtaa tggatacctt
    20101 accgtcacgc accgcaggcc agataaactg gccttcctcc agacgtttgg tgaacaggca
    20161 cagaccatca gcatcagccc acagaatttt aatcgtgtca ccccgtcggc cgcgaaagat
    20221 aaacaggtga ccggagaagg ggttctcatc cagcacatgt tgtacctgtt ctcccagacc
    20281 gttgaaggat ttacgcatat cagtaacgcc ggcaaccagc cagattcgag tgcctgatgg
    20341 gagcgagatc atcgtcccct cccggtcagt tcacggatca acaccgtgag cagctctggt
    20401 gaaggatttt ccagcgtcat gttaccgtgg cggaactcaa ctttacagga actggcactg
    20461 actgtgcttt gtgaaggagt ggataaaagc ggagtaagag ccgccatagg ctctttctgc
    20521 tcatcaggcg ttatctcaac aggtaataat tcaacgccag cgccagaaga ggttgttacc
    20581 ggaagacgcc gcgatatacg cctttcgttc tgccagagcc tgagccattt gaacaggagg
    20641 ttatcattga tatcgtgttc ccgggcaata cgggcaacag aggctcctgg ttgtgaagcc
    20701 agtttaacca tttgaagttt aaactcattt gaaaatgatc tgcggggttc tgaggataat
    20761 attttctgtt ccataacagg tgtccactag ttgaaaaggt gggcacctac gttaccaata
    20821 ctggcttaat ggctacatac ggcggtcagt ttacgcttac gagaaagaag cggtccgccg
    20881 gtcgtctgtt ttgactcgca gaccggacgg gccctcagat atcctgaggc ctggctgcag
    20941 ggctggtgtg gcggtacgct ggtgagtgac ggatacagcg tgtacaaatc tctggcggat
    21001 aatgaccccg gtattacatc tgcctgttgc tggagccacg caaggcgcgg tttcgcaaat
    21061 ctgtataagg ccagcaggga gccccgtgca gccatggcgc tgagaaaaat cgcgggtctt
    21121 tatcgcattg agaagttcat ccgtgaacgt cctgtggaaa aaatacggca atggcgacaa
    21181 cggtattccc ggccgatagt gaatgacctg tttgcctggc ctgaagagca ggagccgtgc
    21241 tgtccgcccg acggaccact aaacaaagcg attaattata tcctgaaccg gcgcgatgaa
    21301 ctgagctgct tcctgagtga cggagccgtg cccctggaca acaatatctg cgagcgtgct
    21361 atccgcccgg tggtcatggg gcggaaagca tggttattcg ccggttcact gatggccggg
    21421 aaccgcgcgg cacagataat gagccttctg gaaacggcaa aacgtaatgg tctggagccg
    21481 catgcctggc tgacagacgt tctgacgcgt ctaccggagt ggccggaaga ccgactggaa
    21541 gagttgctgc cccttgaggg atttaccttc tccgggtgag tgatacctgc catcaggtgt
    21601 tcgtgcagcg ggccataacc cgcagtcggg agttgaacgc ctgacgacag gaaatgagcc
    21661 agagcagcga ccacgcgggc tgacagtgag tcagaacctg aatcagcccg gagccattcc
    21721 cggggctgta cttcatcacc tgccatcagt acccgtgtca gtacttcgcg acctgacgca
    21781 attacccgac tcagttcttc agaacccgcc tccgatacca gcctggcggt cgccccggcg
    21841 caacagaata aatcaaccat cgtcagactg cgatcattcg tatgcatcgc tgatgatgcg
    21901 cccgaaaaac gcgtcaatat gagttcgccg gaatattact gacgacacgg tttgtagtta
    21961 cgttccttcg gtgagtgagt acctgtgcgt cagggtaacg caatcagttc aggtttagcg
    22021 gggtgtcaag tcggtttggc taaccgaccg cagaattaac tgtaccttta aaggtcgccc
    22081 ccgatatatc tgcctgtcag acatatcgcc gattcggtat ctcactacat aaaactcctt
    22141 aaaaaacaat gcgattctgt tggtacgaag ctttcagtaa gtcagtccgg agaagcgcca
    22201 gtacgataca gaatgaggtt gaaaaaaacg ttcacgtctg gtcacttctt caaatgttaa
    22261 aaaacatctc gtaaagagcc agtttatg
//
LOCUS       DAZFXT010000075        21908 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.075, whole genome shotgun sequence.
ACCESSION   DAZFXT010000075 DAZFXT010000000
VERSION     DAZFXT010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.075"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            355..1026
                     /locus_tag="ACHKW0_003076"
     CDS             355..1026
                     /locus_tag="ACHKW0_003076"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HFO7475868.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            1036..2232
                     /gene="yaiP"
                     /locus_tag="ACHKW0_003077"
     CDS             1036..2232
                     /gene="yaiP"
                     /locus_tag="ACHKW0_003077"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO7475869.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2210..2791
                     /locus_tag="ACHKW0_003078"
     CDS             2210..2791
                     /locus_tag="ACHKW0_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7475870.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2793..3566
                     /gene="yaiO"
                     /locus_tag="ACHKW0_003079"
     CDS             2793..3566
                     /gene="yaiO"
                     /locus_tag="ACHKW0_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HFO7475871.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3751..4026
                     /gene="frmR"
                     /locus_tag="ACHKW0_003080"
     CDS             3751..4026
                     /gene="frmR"
                     /locus_tag="ACHKW0_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HFO7475872.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            4061..5170
                     /gene="frmA"
                     /locus_tag="ACHKW0_003081"
     CDS             4061..5170
                     /gene="frmA"
                     /locus_tag="ACHKW0_003081"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HFO7475873.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5263..6096
                     /gene="fghA"
                     /locus_tag="ACHKW0_003082"
     CDS             5263..6096
                     /gene="fghA"
                     /locus_tag="ACHKW0_003082"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018738 - S-formylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0046294 - formaldehyde catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7475874.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQDAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLNYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIHYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6221..6760)
                     /gene="yaiL"
                     /locus_tag="ACHKW0_003083"
     CDS             complement(6221..6760)
                     /gene="yaiL"
                     /locus_tag="ACHKW0_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HFO7475875.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6862..8073)
                     /gene="mhpT"
                     /locus_tag="ACHKW0_003084"
     CDS             complement(6862..8073)
                     /gene="mhpT"
                     /locus_tag="ACHKW0_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HFO7475876.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKSILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8348..9361)
                     /gene="mhpE"
                     /locus_tag="ACHKW0_003085"
     CDS             complement(8348..9361)
                     /gene="mhpE"
                     /locus_tag="ACHKW0_003085"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HFO7475877.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9358..10308)
                     /gene="mhpF"
                     /locus_tag="ACHKW0_003086"
     CDS             complement(9358..10308)
                     /gene="mhpF"
                     /locus_tag="ACHKW0_003086"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HFO7475878.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10305..11114)
                     /gene="mhpD"
                     /locus_tag="ACHKW0_003087"
     CDS             complement(10305..11114)
                     /gene="mhpD"
                     /locus_tag="ACHKW0_003087"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HFO7475879.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVAQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQA
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11124..11990)
                     /gene="mhpC"
                     /locus_tag="ACHKW0_003088"
     CDS             complement(11124..11990)
                     /gene="mhpC"
                     /locus_tag="ACHKW0_003088"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HFO7475880.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSMAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(12008..12952)
                     /gene="mhpB"
                     /locus_tag="ACHKW0_003089"
     CDS             complement(12008..12952)
                     /gene="mhpB"
                     /locus_tag="ACHKW0_003089"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydroxyphenylpropionate/2,
                     3-dihydroxicinnamic acid 1,2-dioxygenase"
                     /protein_id="HFO7475881.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12954..14618)
                     /gene="mhpA"
                     /locus_tag="ACHKW0_003090"
     CDS             complement(12954..14618)
                     /gene="mhpA"
                     /locus_tag="ACHKW0_003090"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008688 - 3-(3-hydroxyphenyl)propionate
                     hydroxylase activity [Evidence IEA];
                     GO_process: GO:0019622 - 3-(3-hydroxy)phenylpropionate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HFO7475882.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVDDVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14809..15642
                     /gene="mhpR"
                     /locus_tag="ACHKW0_003091"
     CDS             14809..15642
                     /gene="mhpR"
                     /locus_tag="ACHKW0_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HFO7475883.1"
                     /translation="MQNNEQTEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLH
                     RTTVRRLLETLQEEGYVRRSPSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLR
                     EVVWPTDVSTLDVDAMVVRETTHRFSRLSFHRPMVGRRLPLLKTASGLTWLAFCPEQD
                     RKELIEMLASRPGDDYQLAREPLKLEAILARARKEGYGQNYRGWDQEEKIASIAVPLR
                     SEQRVIGCLNLVYMASAMTIEQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHL
                     R"
     gene            15719..16801
                     /gene="lacI"
                     /locus_tag="ACHKW0_003092"
     CDS             15719..16801
                     /gene="lacI"
                     /locus_tag="ACHKW0_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HFO7475884.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDATAVEAACANVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16924..19998
                     /gene="lacZ"
                     /locus_tag="ACHKW0_003093"
     CDS             16924..19998
                     /gene="lacZ"
                     /locus_tag="ACHKW0_003093"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HFO7475885.1"
                     /translation="MTMITDSLVVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSTFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEASDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWNKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAANLSVTLPSAPHAIPQ
                     LTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDEKQLLTPLRDQFIRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTVHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGSHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            20050..21303
                     /gene="lacY"
                     /locus_tag="ACHKW0_003094"
     CDS             20050..21303
                     /gene="lacY"
                     /locus_tag="ACHKW0_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HFO7475886.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21369..>21908
                     /gene="lacA"
                     /locus_tag="ACHKW0_003095"
     CDS             21369..>21908
                     /gene="lacA"
                     /locus_tag="ACHKW0_003095"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HFO7475887.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCR"
BASE COUNT         5172 a         5896 c         5634 g         5206 t
ORIGIN      
        1 gcattgtata tatcactgta tcattctcaa tcactcgcat attacattca cataatgtat
       61 ataaacaatg cttgttcaca gaacatattt tttaaatgca attaattgac aaactaaatc
      121 acatctaaat acaatgtata aaacaaaata actatacata ccaaacatac aaacacttaa
      181 catatgaatt gcatagaaac attacccagt cgaacctccg ggtctgaaag cccttgcttt
      241 tcagttatta gcaacattta acatttataa attaacatct ggctgcgcca gagattacgt
      301 ctctgagtgc atcgttgtgc ttgtagtata ataaataaaa tggagtgtca ttaagtggat
      361 aaggttttag attcagccct cctttcttcg gcaaataaaa gaaagggtat ccttgctatt
      421 ggcgcacatc ctgacgatat agaattaggc tgtggcgcat cgcttgctcg tcttgcgcaa
      481 aaaggaattt atatcgcagc cgtggtgatg actaccggca actctggcac agatggaata
      541 atcgatcgcc atgaagaatc gcgcaacgcc ctaaagatat tagggtgcca ccaaactatt
      601 catcttaatt ttgctgacac ccgcgctcat ttacagctca atgatatgat ttccgccctg
      661 gaagacatca ttaaaaatca aattccttct gatgttgaaa tcatgcgggt atataccatg
      721 catgatgccg accgccatca ggatcatctc gctgtttatc aagcttcaat ggttgcctgc
      781 cgcactattc cacaaattct cggctacgaa accccaagta cctggctttc atttatgcct
      841 caggtttttg aatccgttaa agaagaatat ttcacagtca aacttgctgc attaaaaaaa
      901 cataaaagcc aggaacgacg cgattatatg cgtcatgatc gcctgcgtgc agttgcacaa
      961 tttcgcgggc aacaggtcaa tagcgatctg ggtgaaggct ttgtaattca taaaatgatt
     1021 ctttgagcaa attgcatgaa aacctggata tttatctgta tgtccatagc aatgttgcta
     1081 tggtttttaa gtacgctaag acgtaaaccc agtcaaaaga aaggctgtat tgacgccatt
     1141 atacctgcgt ataacgaagg cccgtgtctg gcgcagtcac tggataatct actgcgtaac
     1201 ccttattttt gccgggtaat ttgcgttaac gacggctcca cggacaatac cgaagcggtc
     1261 atggcggaag tcaaacgcaa atggggcgac cgctttgttg ccgtcacgca aaaaaatacc
     1321 ggtaaaggtg gtgcgctgat gaatggcctc aattatgcca cctgcgacca ggttttttta
     1381 agtgatgccg acacctatgt tccgcccgat caagacggaa tgggctatat gctggcagaa
     1441 attgagcgcg gtgctgatgc cgtaggcggc attccctcta ctgcgttgaa aggcgcgggg
     1501 ctgttaccgc acatccgcgc gaccgtaaag ttgccgatga ttgttatgaa gcgcacgcta
     1561 cagcagctcc tgggcggcgc accgtttatt atcagcggtg cctgcggaat gttccgcact
     1621 gatgtattgc gtaagttcgg tttctcagat cgtactaaag tcgaagacct tgatctcacc
     1681 tggacattag tggcaaacgg ctaccgtatt cggcaggcga atcgctgcat cgtataccca
     1741 caggaatgca acagcccgcg tgaggagtgg cgtcgctggc ggcgttggat tgtgggatac
     1801 gcggtctgta tgcgcctgca taaaagactt ttatttagcc gcttcggtat cttcagtata
     1861 tttcctatgc tgttggttgt gatttatggc gttgggattt atctcactac ctggtttaat
     1921 gaattcatca ccaccgggcc gcatggtgtg gtgttggcaa tgtttccgct tatctggatc
     1981 ggcgtagttt gtgttattgg tgcttttagc gcctggtttc atcgttgctg gttgttggtg
     2041 cctttagcgc cgctttccgt tgtgtatgta ttattagctt atgccatctg gattatttat
     2101 ggacttattg ccttttttac tggacgcgaa cctcagcgcg acaaacccac ccgctattcc
     2161 gcactggtgg aagcgtcaac cgcttattcc caaccttctg tcacaggaac tgaaaaacta
     2221 tctgaagctt aatgttaaag agaaaaatat tcagattgca gaccaggtaa ttattgatga
     2281 aactgcaggt gaagttgtta tcggcgcgaa tacccgtatt tgtcatggtg ccgttattca
     2341 gggtccggta gtgattggcg caaactgcct gataggtaat tatgcgttta ttcgtcctgg
     2401 cacaataatc agcaatggtg taaaaattgg ttttgccacc gaaattaaaa atgcggttat
     2461 tgaagcggaa gcaacgattg gtccgcaatg ttttattgcc gactcggtag ttgcaaacca
     2521 ggcatatttg ggcgcacaag tacgtaccag taatcatcgt ctggatgaac agcctgtgtc
     2581 tgttcgaact ccagagggaa ttatcgctac cggatgcgat aaattaggtt gttatatcgg
     2641 gcagcgttca cgccttggcg tacaagttat aattttgcct gggcgaatta tttccccgaa
     2701 cacgcaactt ggcccgcgcg tgattgtaga acgtaattta cctgccggaa cttattcact
     2761 ccgacaagaa cttatccgta caggagatta aaatgataaa acggacgtta ttagcggcgg
     2821 ccatttttag cgcattgccc gcttatgccg ggttaacttc cattaccgcg ggctacgatt
     2881 ttaccgatta ttctggcgat catggcaacc gtaatttagc gtatgctgaa ctggtggcga
     2941 aagttgaaaa cgcaacgctg ctttttaatc tttcccaggg gcgtcgtgat tatgaaactg
     3001 aacatttcaa tgccactcgc ggtcagggtg cggtctggta taaatggaat aactggctga
     3061 caacccgaac gggtattgcc tttgcggata atacgccggt ctttgcccgc caggattttc
     3121 gtcaggatat taacctggcc ctgttgccaa aaacgctttt cacgaccggt tatcgctaca
     3181 ctaaatatta cgatgatgtc gaagtcgatg cctggcaagg cggcgtatca ctctatactg
     3241 gcccggtgat caccagctac cgctataccc attatgactc cagcgatgca ggtggtagtt
     3301 atagcaatat gatttccgtg cgtctgaatg acccgcgcgg cactggttat acgcaactat
     3361 ggctaagccg cggaacaggc gcttacacct atgactggac gccagaaaca cgctacggca
     3421 gcatgaagag cgtcagtttg caacgtattc aaccgctgac tgagcaactt aatctcgggc
     3481 tgacggcagg taaagtgtgg tacgacaccc caaccgatga ttataacggt ctgcaacttg
     3541 cagcccatct gacctggaaa ttctgattcc ttctgccgcc cgctatccgg ggcggccttc
     3601 cctgccgatt agccccccct ttcctctttg ttttccgacc acattcaccg gataaatttt
     3661 attctccagt gttatatact ataggggggt atgcattgac atatagcata cccccctata
     3721 gtatattgcg tgcagatgat gaggtgcgaa atgcccagta ctccggaaga gaagaaaaag
     3781 gtccttactc gagttcgtcg tattcgaggg cagattgatg ctctggaacg gtcgctggag
     3841 ggtgatgccg aatgccgtgc catactccaa cagatcgctg ccgttcgggg cgcggctaac
     3901 gggctgatgg cagaagtgct tgaaagccat atccgggaaa cgtttgaccg aaatgactgc
     3961 tacagccgcg aagtcagcca atccgttgac gacactattg aactggttcg agcctatctt
     4021 aaatagctga atctattacc atattgagga agagcgagag atgaaatcac gtgctgccgt
     4081 tgcatttgct cccggtaaac cgctggaaat cgttgaaatt gacgttgcac caccgaaaaa
     4141 aggtgaagtg ctgattaaag tcacccatac cggcgtttgc cataccgacg catttaccct
     4201 ctccggggat gacccggaag gtgtattccc ggtggttctc ggtcacgaag gggccggcgt
     4261 tgtggttgaa gtcggtgaag gcgtaaccag cgtcaaacct ggcgaccatg tgatcccgct
     4321 ttacaccgcg gagtgcggcg agtgtgagtt ctgtcgttct ggcaaaacta acctctgtgt
     4381 tgcggttcgc gaaacccagg gtaaaggctt gatgccagac ggcaccaccc gtttttctta
     4441 caacgggcag ccgctttatc actacatggg gtgctctaca ttcagtgaat acaccgtggt
     4501 cgcggaagtg tctctggcca aaattaatcc agaagcaaac catgaacacg tctgtctgct
     4561 gggctgtggc gtgaccaccg gtattggcgc ggtgcacaac acagctaaag tccagccagg
     4621 tgattctgtt gccgtgtttg gtcttggcgc gattggtctg gcagtggttc agggcgcgcg
     4681 tcaggcgaaa gcgggacgga ttatcgctat cgataccaac ccgaagaaat tcgatctggc
     4741 gcgtcgcttc ggtgctaccg actgcattaa cccgaatgac tacgacaaac cgattaaaga
     4801 tgtcctgctg gatatcaaca aatggggtat cgaccatacc tttgaatgca tcggtaacgt
     4861 caacgtgatg cgtgcggcgc tggaaagtgc gcaccgcggc tggggtcagt cggtgatcat
     4921 cggggtggca ggttctggtc aggaaatctc cacccgtcca ttccagttgg tcactggtcg
     4981 cgtatggaaa ggttccgcgt ttggcggcgt gaaaggtcgt tcccagttac cgggtatggt
     5041 tgaagatgcg atgaaaggtg atatcgatct ggaaccgttt gtcacgcata ccatgagcct
     5101 tgatgaaatt aatgacgcct tcgacctgat gcatgaaggc aaatccattc gaaccgtaat
     5161 tcgttactga tttcccgcag gtataccccg tccacttcag acggggttct taatactctc
     5221 cccgggcagc cgtccggggg attaaccgtg agataatgac tgatggaact cattgaaaaa
     5281 catgccagct ttggcggctg gcaaaatgtg tatcggcatt attcccaatc actgaaatgt
     5341 gaaatgaatg tcggcgtcta tctcccacca aaagccgcga atgaaaaatt gccggtgctg
     5401 tactggcttt cgggcctgac ctgcaacgag cagaatttca ttactaaatc ggggatgcag
     5461 cgttacgcgg ctgaacacaa cattattgtt gttgcgccgg acaccagtcc gcgaggcagt
     5521 catgtcgcag atgccgaccg ttacgatctc gggcaagatg ccgggtttta cctgaacgca
     5581 acgcaagcgc cgtggaatga acattacaaa atgtatgact atatccgcaa cgagctgccg
     5641 gatttagtga tgcaacattt tccggcaacg accagaaagt ctatctctgg tcattctatg
     5701 ggcgggctgg gcgcgctggt gctggcgtta cgtaacccag atgaatatgt cagcgtctcg
     5761 gcgttttcgc ccattgtctc cccatcgcaa gtaccgtggg gacagcaagc ctttgccgca
     5821 tatcttggtg aaaataaaga tgcctggttg aattacgatc cggtgagtct tatttcacaa
     5881 ggtcaacgcg ttgcggaaat catggttgat caggggttga gtgatgattt ttacgcggaa
     5941 cagctgcgga ctccaaatct tgaaaagatc tgccaggaga tgaatatcaa gacgttaatc
     6001 cattatcacg agggttacga ccacagctat tattttgtct ccagttttat tggcgagcat
     6061 attgcctacc acgccaataa actgaatatg cgttgataat agtgcacgac tgccggatgc
     6121 ggcgtgaacg ccttatccgc ctacatttcg cccgcaggcc gtaggcatga taagacgcgc
     6181 atagcgtcgc atcaggcatc tgcgcacgac ttatctatgt ttaccacatc aaatcatcag
     6241 gcactttgaa gtcggcatat gggtcgtctt catcctgctc ttcggcactg agcgcgctgt
     6301 gcaacacaat actgctggca tcacgctgag caattttatc ggcaacgctg gcgggaataa
     6361 tggcgtattc accttcgcta ttgttatcga ccaacaagcg ggcaatcgcc agacgaccat
     6421 taatcaactg cgcctgtgtg agcttatcga caaaaatctt cttaatcaga ttgccgtcag
     6481 tgaagttaaa accaatatca ccattggcaa tggtgattcg gttcatttca ataagctgct
     6541 tcacctgggc tttatattct ttcgccaacg ccgcttgttt ttgctgttcg ctaagctgtt
     6601 tatcacgctc aagctgtgcc tttttatttt cttctaccgc cgcccgagct tcacgcgcct
     6661 gaacgcgcga ttttttcgcc gttctctcga ccttcgccgc ttttttgctg gtgactaatc
     6721 ctgctttgag caactgctct tgtaaggtaa gttttgccat ctttgtttcc aaacccgctg
     6781 aataattttt agcgagtata cctgtaaacg ccgtgggtgt attgggggca agtccctttc
     6841 gcccctgcta aataaggcgc gtcaggcatc ggcacacggc tgcattcttg atctccgact
     6901 catcaaaata aacaccgcca acccagcaac aagaataccc ggtgcagaag cggccattac
     6961 gccgaccgtg ccagtgccta atgccagcat tttcccggcc agtaacggac cgctcatagc
     7021 ccccagacgc cctaccgcca cggctgttcc cacacctgtt gcgcggatct gcgaactgta
     7081 aaacaacggt gccagggcat acaaaacgct ttgcccacct gtcgcaaaca accccgcgac
     7141 aaatcccgcc agcaacatac cgttaaatga cgacaccgtt ccaagcgcca gcagcgaagc
     7201 taacatgccg ctataaatca gtagcgacat ggttactgga cgcagcttat ccatcaatgc
     7261 gcccaacatt aacgtcccgc ttgccgcccc catttgcagg gcaaacatca cccctgccgc
     7321 ctgcgatggc tggaatcctt gctccaccaa aagtagcggt agccagttga tcaacatata
     7381 aaccaccagc agagtgaaga aataacacaa ccacagcagc agcgtcgcgg ttgccgtttc
     7441 tggcgcaaat aaggcacgca gtggtggcgc agcctgtttt tcgccagcga aaaccgctga
     7501 ctccggcagc cagcgcatta atagcggcac cagaatcaac ggcaccacac cacctaccca
     7561 aaacaccgtt tgccatgcta agtttgcccc cgcgaaaccc agtgtcgccg ccagcgccgc
     7621 gccaatggga acaccgcaat acatcaggct cactgccgtc ccacgaaaac gtggacccgc
     7681 ggcttcagac gtcagggcga taagattcgg caacgccgcc cccagcccga caccggtcat
     7741 cagccgcgca aagaccagtg aggggaaatc ccaggcaatc gccgttgcca gtgagaacaa
     7801 accaaacagc gcaactgagc caatcaaaat actcttacga ccataacggt ctgccagcat
     7861 cccgccaacc aacgcgccgg gtagcaaacc gagtattccg gcgctaaata tccagcccat
     7921 ttgcatttta tcgagtgcga aagcctgggc gatgccaccc gccgcaatgc cagccgcctg
     7981 aagatccagc ccttccatca gagcgaccaa aaaacaaagc ccgatggtca gcatcaggcg
     8041 ggatgaagat gatgaagggg tacgagtcga cataagatga acctcgttgc gagtgcaggt
     8101 gaatcgcgac tgatacattg ccagccgcgt aatcagaact atttaatgac tgcctacggg
     8161 cgaaatgtag gccggacaag acgtttatcg catccggcag tcgtgtgcag atgcctgatg
     8221 cgacgctaac gcgtcttatc aggcctacaa attctcacgc cacccgtagg ccggataagg
     8281 tgtttacgcc gcatccggca gtcgtgctgg gatgggcata caaaatgcac gttctcggca
     8341 gtcatcatta tttgttgttg cgcagatcca gcgccacgtc aacgatcata tcctcctggc
     8401 cgccaaccat ccggcgtttg cccagctcaa cgagaatatc caccgcactt aagccataac
     8461 gcgccgccgc cgtttcacag tgacgcagga agctcgagta aacaccagcg tatcccagcg
     8521 ccagcgtttc gcgatcgact cgtaccggtc gatcctgcaa cggacgcacc aggtcgtcag
     8581 cggcatccat taacgcatag agatcggtcc catgctgcca gcccagttta tccgcggcgg
     8641 caataaacac ttccagcggt gcgttacctg cgcccgcgcc cattcccgcg aggctggcgt
     8701 cgattcggtc gcagccctct tccaccgccg cgatagagtt cgccacgcca agactcaggt
     8761 tatggtgagc gtgcatgcca gtttgcgttt ctggtttcag ctctgctttc agggcgcgga
     8821 aacggtcacg gatatcgctc atgttcatcg caccgccaga atccaccaca taaatacagg
     8881 tcgcaccgta gccttccatc agctttgcct gctttgcgag attctccggc gtggtcatat
     8941 ggctcatcat cagaaaacca acggtgtcca ttccgagctc gcgggcatac tgaatatgct
     9001 gggcggaaac atcagcttcg gtacagtgcg ttgccacacg aaccacccgc gcgccagcct
     9061 gccaggcatt tttcagatcg tgaatagtgc cgattcctgg cagcaacaac gtcgcgattt
     9121 tggcgtgctt caccacatcc gccgctgctt caatccattc aaggtcgcta tgtgcgccga
     9181 aaccatagtt aaagctggaa ccctgcaaac cgtcgccatg ggccacttca atcgaatcca
     9241 cgcgggcatc gtccagtgct ttggcaatct ggcgaacgtt ttccagcgaa tactgatgac
     9301 gaatggcgtg cataccgtca cgcaacgtga cgtccgagat ataaagtttt ttaccgttca
     9361 tgccgcttct cctgccttgc gcgccagtga ctgggccatt ttttccgctg tcgccagcgc
     9421 actggaagtc ataatgtcga ggttgcccgc ataggcaggc agataatgcg ctgcgccttc
     9481 gacttccagc cagaccgctg ttttcagtcc ggagaattgc cccacgcccg gtaaattgac
     9541 cggtttatcc tgcgggataa cttcaaactg cacgcgctgt ttcaggcgat aacccggtac
     9601 gtaagcctgc accgcctcag ccatttcatt gattgaggct tcgatatcat cttgtgaagc
     9661 ttcgtcgctc aatacataca ccgtgtcacg catcatcaac ggtggctctg ctgggttaag
     9721 cacaataatc gccttccctt ttgccgcgcc gcccaccact tcaatggctc gggaagtggt
     9781 ttccgtaaat tcatcgatat tggcacgcgt gccaggtccg gcagatttac tggcgataga
     9841 agcgataatt tcggcgtaat gaacacgcgc cacgcgtgaa actgccgcca ccattggaat
     9901 ggtggcctgg ccgccgcagg tgaccatgtt gacgttcagt tgatcgacgt tcgcctcgag
     9961 gttaaccacc ggcacgcagt aagggccgat ggcagcaggc gtcaggtcaa ttaagcgaat
    10021 atccggtttc gcttcgcgta aagcggcatc gtttttcaca tgcgcgcccg cgctggtcgc
    10081 atcaaataca atgtcgatat cagcaaattc aggcatgttc atcagcccga tcaccccttc
    10141 atgggtggtg gcgacgccca tacgtctggc acgcgccaga ccgtcggact gaggatcaat
    10201 gccgaccatc accgccatct ccagatgctg accgtgacgc aaaattttaa tcatcagatc
    10261 ggtgccaatg ttgccagaac cgataatggc gactttacgc ttactcatga cagacttcct
    10321 tttggggctg cgcttgaaaa tgtcgcagca actgaaccta tgccttcaat atgggcttca
    10381 aaacgatcgc ccgcattcac tgccaccatc ggacctaatg ccccggtaag aatgatatct
    10441 ccggcgcgca gcggttcacc caggctggcc attttgcgtg ccagccagac ggcggcatta
    10501 agcggatgtc ccaggcattc gctgccgcgc ccgctggaaa cctcttcgtt attacgcgtc
    10561 atcttcatgg cgcagttttt caggtctaac cccgccggac gttgcgccgg accgccgatg
    10621 acatacaccc cgcaggaagc gttatctgcc acggtatcga caaactgaat cgaccagtcg
    10681 cgaatgcggc tacccaccac ctccagcgcc ggaagtaccc attcaatggc gttatacagt
    10741 tcgtcgaagg tgatatcggt tgcgggcaaa tcgcggttca acaccagtgc gatctccgct
    10801 tcaatgcggg cttgcagaac acgggaaaaa ggaatgattt cgttatcgcc ataacacatg
    10861 tcggcaaata atgtcccaaa atccggttga tcaacgccca gttgttgctg cactttcgga
    10921 tgcgtcaggc ccactttacg ccctaccacg cgacgcccct gcgcaacgtc atattgcaca
    10981 tttatgtgct gaatggcgta ggccgcttca gcgttatcga taccaatcag atcgcgcagc
    11041 ggcgcaatcg cttcgccctg ctctgcggcg cggcgtaaat ccgccgccag ttgctcaaga
    11101 gtatgcttcg tcatgaccat tccttaaggg cgtgcgagga aattcagcac caattgattg
    11161 aaagcgtcgg catgttccca ctgcgcccag tgaccacagt cgcggaagat atgcagttca
    11221 gaaccggcaa tgccggacag cagacgcaga cccgcatcca tcggcacaaa gcggtcgttg
    11281 cgcccccaga caatcagggt ttgcgctttg atttccgcca gacgtgggcc aaaatccggg
    11341 aactgtttcg gattagcttc caggctctta acgaagtttt ccaggtgatc gcggcgcgac
    11401 agcatattat tcaggcgcgc ttcaaacagg gcgtcggtca aatcgctggt atcgaaaacg
    11461 aagatatcca tcatcagctt caggttttcg atagtcggct gacgataaag ctgattcagt
    11521 cgcttaatac cttcggttgg catcggcgta aacaaactca tgccgcccgt accgccgccc
    11581 atcagcacca gtttgccgac gcgctccggc cagttaaggg tgaacgccat agaactatgg
    11641 ccgcccatcg agttgcccag caggtggatt ttggcgatat ccagttgatc caccacgctt
    11701 ttcaggattc gtgcattaag atccgatcgt gaaccactat taacgatcga atcgcttttg
    11761 ccccaacccg gacaatccag caggattacc cgatagcccg cctctaccag cggatcgata
    11821 ttgcggctga agttcgccca gccagtagca cccgggccgg aaccgtgcag caggacaacg
    11881 gtttcgtcgc cttgtccgca gtcattaaaa tggatgcgca gcgttttacc cgcttcttct
    11941 acattcagaa aacggctggt ggcggcttcg gtttgtggct gataactcat catcttctcc
    12001 tgcatattca gttctctgtt ctggcgctta acgagccaaa tccggcaatc cactccggga
    12061 ttgggcggta ataacgccct tcgctacgcc agttgccaaa tgcagaaata gcggcaaaag
    12121 cggcgaccca ggttttgatt tcatgtgtcg actttccggc aatggcggaa agctcttcgt
    12181 tactgacggc atccagttcc tgtatgcgtc cctgctccag caaagtcatg aactggttat
    12241 cccaaatcgg gttgagcgga tgcagagttc tctgatcctc aacaaacttc tcagcggcgc
    12301 taatcacccg ttgctgacgc aattcgcgct cactggcggg taaatctttc ccgctcccca
    12361 acagacggtc gcgcatatgg gcatcggctt tcgccagttc gggcaccggc ggctgatggg
    12421 aaagcccacc ggaacccagg aacagcacgc gtttattgag agtgctggtg aaacgtccaa
    12481 tggcttcacc caacatgcgg gtacgctgga aaccgggcag cggcgtggcg acaccgttga
    12541 tgaacacagg cagaactggc accttatcca gcccgccgag caggaactcc agtggctggg
    12601 cgaacccgtg gtccacctgc atacagtaag aaacggcaag atcgatcccg ctcttcatga
    12661 cggcatgcgc acaggcctcc gccagctcca caggcactgg cagctctcct gccgcactgc
    12721 cgaaatcacc aattgccgtc gctccaacgc ctaaacagaa cggtggcatc acgtcataga
    12781 aaaagccgtt gtagtgatct ggcgcaaaca gcaccaccag ttcaggggag aaagccgcaa
    12841 tacgctcgcg ggcgctggca atcacgccat tgacctcatc gagcacctct tgcgccgggt
    12901 cgacataccc caccagcggc gagtgggaaa gacagtgaag ataagcgtgc atatcaggct
    12961 accttttcga cagaaacgtc ggcatcaggg cgggtcagcg tcatcaccga cgccagttta
    13021 ttcagggtct tgcccagggt ttgcggaatg gcggtggcgg caacaaagcg atccgggcgc
    13081 atcaccacca gcgaagcatt gtgttgcgcg aaccagctac gcagacgacc ttgcgtatcg
    13141 cccacgcgta gcacgccgtc gtggttatcc tgtgcggtat gaatttgcac ttccggcacc
    13201 acctgaatga agcgtgtgcc caacgcgcgc cactgctgga tttgctcgtc gctcatcccc
    13261 cacagtggat tgcatcccca gccaattacc gcgaagttcg cgccaatcgc gttatcgagc
    13321 agcgtcacgt caccgttttc cagcgtgact ttcggctgaa taaacatctt gccgaccgga
    13381 gagtgcttcg cttcgccctc acgcaccagc gcaccgccgt aatattgcgg catcggcttg
    13441 aagcgcattt cgaggaaata gcgttttact ggcggcagat aattcaacag ccaggaaacg
    13501 ccgtcacgta acgtaccctg ccagcgtttc ggcggagcca gcacgttgcc cgccgtcacg
    13561 gacaggtcaa tcatcgcttt ggcgtgatcg cgacgttctt gttgataggt atcgagcagc
    13621 gcatcgcggg ctttcccctg gataaccaac gccagtttcc aggcgaggtt aaaggcgtcg
    13681 cgcataccac tgttatagcc ctgcccctgc cacaccggca tgatgtgcgc ggcatcgccc
    13741 gccagcagta cgcgatcaat acggaaacgt tgcgccagtc gcgcgttatg ggtgtagaca
    13801 cgctggcgaa tcaattcaac attgtccgga ttaggcagca ctttgcttaa cagcttgcgc
    13861 atattttgcg gctcacgcag ctgctcttcg gtttctcccg gcatcaccat aaattcaaag
    13921 cgacgtaccg catgaggcag cgcggcagaa acatacgggc gcaccggatc gcaacacaaa
    13981 tagatatgcg gcgtacttaa cggatcgttg gcgatatcta ccacaatcca ctgatttggc
    14041 gcagttttac cttcaaacgg cacattcaga gtgcgacgga caaaacttgc tccaccgtca
    14101 caggctacca gccactgggc tttgaccgtt tcccgctgcc cttctgccgt tttcaggtgc
    14161 aaggtcactt cgtcattttg ctggctgaag gcctccagct cgcgggaaaa caagcagcgc
    14221 acattcggaa aacgcgacaa cccttccagc atcaccgcat cgacctgagg ctgaataaag
    14281 gcgttacggc gcggccagcc aaattcatcg gtcattggct gaatatcagc aaaacagcgg
    14341 cctttcgggg tgagaaaacg catcgcgtgc cacggcgtag tgtgcggcag aacatcatcg
    14401 accaggccga ccgactgcat ggtgcgcagc gcctcgtcat caataccaat cgcacgcggg
    14461 tagtcgatca acttatcgag tttttccacc accagcacgt caatgcccat ctggccgaga
    14521 tagttcgcca tcatcagccc gaccggaccg gcaccagcga tcgccacctg aacgctatgg
    14581 ttaacagcag gctggatgtc agggtgttgt attgccattt cagtacctca cgactcggac
    14641 aaaatgccgt tgcgcgcaca gtacggcgca acttattttg ttaaaaacat gtaaatgatt
    14701 ttttattgtg cgctcagtat aggaagggtg ttttcggcta caatcaaaac atgcccgaat
    14761 gtgcaccagg tgcacaactt tgttttaact atagaaatgt caattaatat gcagaacaat
    14821 gagcagacgg aatacaaaac cgtgcgcggc ttaacccgcg gtctaatgtt attaaatatg
    14881 ttaaataaac ttgatggcgg tgccagcgtc gggctgctgg cggaactcag cggcctgcat
    14941 cgcaccactg tgcggcgact gctggagacg ctgcaggaag agggatatgt ccgccgtagc
    15001 ccctccgatg atagttttcg tctgaccatc aaagtgcggc aattaagcga aggatttcgt
    15061 gacgaacagt ggatttctgc actggcggcc ccactgctgg gcgatctgtt gcgcgaagtg
    15121 gtatggccga cagatgtgtc cacgctggat gttgatgcaa tggtggtacg cgaaaccact
    15181 caccgtttca gccgcttatc ctttcaccgg ccaatggtcg ggcgacgttt gccgcttctg
    15241 aaaaccgcct ctggcctgac ctggctggcc ttttgcccgg aacaagaccg caaggaatta
    15301 atcgaaatgt tagcctcccg ccccggtgat gactatcaac tggcacggga accgttaaag
    15361 ctggaagcca ttctggcgcg cgcgcgcaaa gagggttacg gacagaacta ccgcggctgg
    15421 gatcaggagg agaagatcgc ctctatcgcc gtaccgctgc gcagtgaaca acgggtgatt
    15481 ggctgtctga atctggtgta tatggcgagc gcaatgacca ttgaacaggc agcggaaaag
    15541 catcttccgg cgctacaacg ggtagcaaaa cagatcgaag aaggggttga atcgcaggct
    15601 attctggtgg ccggacggcg aagcggcgtg catttacgtt gacaccatcg aatggcgcaa
    15661 aacctttcgc ggtatggcat gatagcgtcc ggaagagagt caattcaggg tggtgaatgt
    15721 gaaaccagta acgctatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
    15781 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
    15841 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
    15901 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
    15961 ggcgattaaa tctcgcgctg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
    16021 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
    16081 gctgatcatt aactatccgc tggatgacca ggatgccact gctgtggaag cagcctgcgc
    16141 taatgttccg gcgttatttc ttgatgtctc tgaccagact cccatcaaca gtattatttt
    16201 ctcccatgaa gacggtacgc ggctgggcgt ggagcatctg gtcgcattgg gtcaccagca
    16261 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggcgggctg
    16321 gcataaatat ctcacacgca atcaaattca gccgatagcg gaacgggagg gcgactggag
    16381 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
    16441 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
    16501 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcgtg
    16561 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
    16621 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
    16681 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
    16741 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
    16801 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
    16861 tgcttccggc tcgtatgttg tgtgaaattg tgagcggata acaattttac acaggataca
    16921 gctatgacta tgattacgga ttcactggtc gtcgtattac aacgtcgtga ctgggaaaac
    16981 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
    17041 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
    17101 cgctttgcct ggtttccggc accagaagcg gtgccggaaa gctggctgga gtgcgatctt
    17161 cctgaggccg atactgtcgt cgtcccctca aactggcaga tgcacggtta cgatgcgccc
    17221 atctacacca acgtgaccta tcccattacg gtcaatccgc cttttgttcc cgcggagaat
    17281 ccgacgggtt gttactcgct cacatttaat attgatgaaa gctggctaca ggaaggccag
    17341 acgcgaatta tttttgatgg tgttaactcg gcgtttcatc tgtggtgcaa cgggcgctgg
    17401 gtcggttacg gccaggacag ccgtttgccg tctgaatttg acctgagcac atttttacgc
    17461 gccggagaaa accgcctcgc ggtgatggtg ctgcgctgga gtgacggcag ttatctggaa
    17521 gatcaggata tgtggcggat gagcggcatt ttccgtgacg tctcgttgct gcacaaaccg
    17581 agcacacaaa tcagcgattt ccatgttgcc actcacttta atgatgattt cagccgcgcg
    17641 gtactggaag cagaagttca gatgtatggc gagctgcgcg atgagctgcg ggtgacggtt
    17701 tctttgtggc agggtgaaac gcaggtcgcc agcggcaccg cgcctttcgg cggtgaaatt
    17761 atcgatgagc gtggtggtta tgccgatcgc gtcacactac gtctgaacgt cgaaaacccg
    17821 aaactgtgga gcgccgaaat cccgaatctc tatcgtgcgg tggttgaact gcacaccgcc
    17881 gacggcacgc tgattgaagc agaagccagc gatgtcggtt tccgcgaggt gcggattgaa
    17941 aatggtctgc tgctgctgaa cggcaagccg ttgctgattc gcggcgttaa ccgtcacgag
    18001 catcatcctc tgcatggtca ggtcatggat gagcagacga tggtgcagga tatcctgctg
    18061 atgaagcaga acaactttaa cgccgtgcgc tgttcgcatt atccgaacca tccgctgtgg
    18121 tacacgctgt gcgaccgcta cggcctgtat gtggtagatg aagccaacat tgaaacccac
    18181 ggcatggtgc caatgaatcg tctgaccgat gatccgcgct ggctaccggc gatgagcgaa
    18241 cgcgtaacgc gaatggtgca gcgcgatcgt aatcacccga gtgtgatcat ctggtcgctg
    18301 ggaaatgagt caggccacgg cgctaatcac gacgcactct atcgctggaa caaatctgtc
    18361 gatccatccc gcccggtgca gtatgaaggc ggcggagccg acaccacggc caccgatatt
    18421 atttgcccga tgtacgcgcg cgtggatgaa gaccagccct tcccggctgt gccgaaatgg
    18481 tccatcaaaa aatggctttc gctacctgga gagacgcgcc cgctgatcct ttgcgaatac
    18541 gcccacgcga tgggtaacag tcttggcggc ttcgctaaat actggcaggc gtttcgtcag
    18601 tatccccgtt tacagggcgg cttcgtctgg gactgggtgg atcagtcgct gattaaatat
    18661 gatgaaaacg gcaacccgtg gtcggcttac ggcggtgatt ttggcgatac gccgaacgat
    18721 cgccagttct gtatgaacgg tctggtcttt gccgaccgca cgccgcatcc agcgctgacg
    18781 gaagcaaaac accagcagca gtttttccag ttccgtttat ccgggcaaac catcgaagtg
    18841 accagcgaat acctgttccg tcatagcgat aacgagctcc tgcactggat ggtggcgctg
    18901 gatggtaagc cgctggcaag cggtgaagtg cctctggatg tcgctccaca aggtaaacag
    18961 ttgattgaac tgcctgaact accgcagccg gagagcgccg ggcaactctg gctcacagta
    19021 cgcgtagtgc aaccgaacgc gaccgcatgg tcagaagccg gacacatcag cgcctggcag
    19081 cagtggcgtc tggcggcaaa cctcagcgtg acactcccct ccgcgcccca cgccatcccg
    19141 caactgacca ccagcgaaac ggatttttgc atcgagctgg ataataagcg ttggcaattt
    19201 aaccgccagt caggctttct ttcacagatg tggattggcg atgaaaaaca actgctgacc
    19261 ccgctgcgcg atcagttcat ccgtgcaccg ctggataacg acattggcgt aagtgaagcg
    19321 acccgtattg acccgaacgc ctgggtcgaa cgctggaagg cggcgggcca ttaccaggcc
    19381 gaagcggcgt tgttgcagtg cacggcagat acacttgccg acgcggtgct gattaccact
    19441 gtccacgcgt ggcagcatca ggggaaaacc ttatttatca gccggaaaac ctaccggatt
    19501 gatggtagtg gtcaaatggc gattaccgtt gatgttgaag tagcgagcga tacaccgcat
    19561 ccggcgcgga ttggcctgac ctgccagctg gcgcaggtag cagagcgggt aaactggctc
    19621 ggattagggc cgcaagaaaa ctatcccgac cgccttactg ccgcctgttt tgaccgctgg
    19681 gatctgccat tgtcagacat gtataccccg tacgtcttcc cgagcgaaaa cggtctgcgc
    19741 tgcgggacgc gcgaattgaa ttatggctca caccagtggc gcggcgactt ccagttcaat
    19801 atcagtcgct acagccaaca acaactgatg gaaaccagcc atcgccatct gctgcacgcg
    19861 gaagaaggca catggctgaa tatcgacggt ttccatatgg ggattggtgg cgacgactcc
    19921 tggagcccgt cagtatcggc ggaattccag ctgagcgccg gtcgctacca ttaccaattg
    19981 gtctggtgtc aaaaataata ataaccgggc aggccatgtc tgcccgtatt tcgcgtaagg
    20041 aaatccatta tgtactattt aaaaaacaca aacttttgga tgttcggttt attctttttc
    20101 ttttactttt ttatcatggg agcctacttc ccgtttttcc cgatttggct acatgacatc
    20161 aaccatatca gcaaaagtga tacgggtatt atttttgccg ctatttctct gttctcgcta
    20221 ttattccaac cgctgtttgg tctgctttct gacaaactcg ggctgcgcaa atacctgctg
    20281 tggattatta ccggcatgtt agtgatgttt gcgccgttct ttatttttat cttcgggcca
    20341 ctgttacaat acaacatttt agtaggatcg attgttggtg gtatttatct aggcttttgt
    20401 tttaacgccg gtgcgccagc agtagaggca tttattgaga aagtcagccg tcgcagtaat
    20461 ttcgaatttg gtcgcgcgcg gatgtttggc tgtgttggct gggcgctgtg tgcctcgatt
    20521 gtcggcatca tgttcaccat caataatcag tttgttttct ggctgggttc tggctgtgca
    20581 ctcatcctcg ccgttttact ctttttcgcc aaaacggatg cgccctcttc cgccacggtt
    20641 gccaatgcgg taggtgccaa ccattcggca tttagcctta agctggcgct ggaactgttc
    20701 agacagccaa aactgtggtt tttgtcactg tatgttattg gcgtttcctg cacctacgat
    20761 gtttttgacc aacagtttgc taatttcttt acttcgttct ttgctaccgg tgaacagggt
    20821 acgcgggtat ttggctacgt aacgacaatg ggcgaattac ttaacgcctc gattatgttc
    20881 tttgcgccac tgatcattaa tcgcatcggt gggaaaaacg ccctgctgct ggctggcact
    20941 attatgtctg tacgtattat tggctcatcg ttcgccacct cagcgctgga agtggttatt
    21001 ctgaaaacgc tgcatatgtt tgaagtaccg ttcctgctgg tgggctgctt taaatatatt
    21061 accagccagt ttgaagtgcg tttttcagcg acgatttatc tggtctgttt ctgcttcttt
    21121 aagcaactgg cgatgatttt tatgtctgta ctggcgggta atatgtatga aagcatcggt
    21181 ttccagggcg cttatctggt gctgggtctg gtggcgctgg gcttcacctt aatttccgtg
    21241 ttcacgctta gcggccccgg cccgctttct ctactgcgtc gtcaggtgaa tgaagtcgct
    21301 taagcaatca atgtcggatg cggcgcgagc gccttatccg accaacatat caggacggaa
    21361 tgatcgcaat gaacatgtcg atgacagaaa gaataaaagc aggcaagcta tttaccgata
    21421 tgtgcgaagg cttaccggaa aaaagacttc gtgggaaaac gttaatgtat gagtttaatc
    21481 actcgcatcc atcagaagtt gaaaaaagag aaagcctgat taaagaaatg tttgccacgg
    21541 taggggaaaa cgcctggata gaaccacctg tctatttctc ttacggttcc aacatccata
    21601 taggccgcaa tttttatgca aatttcaatt taaccattgt cgacgactac acggtaacga
    21661 tcggtgataa cgtattgatt gcacccaatg ttactctttc cgttacggga caccctgtac
    21721 accatgaatt gagaaaaaac ggtgagatgt actcttttcc gataacgatt ggcaataacg
    21781 tctggatcgg aagtcatgtg gttattaatc caggcgtcac catcggggat aattctgtta
    21841 ttggcgcggg tagtgtcgtc ataaaagaca ttccaccaaa cgtcgtggct gctggcgttc
    21901 cttgccgg
//
LOCUS       DAZFXT010000076        21715 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.076, whole genome shotgun sequence.
ACCESSION   DAZFXT010000076 DAZFXT010000000
VERSION     DAZFXT010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.076"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            349..1863
                     /gene="caiT"
                     /locus_tag="ACHKW0_003096"
     CDS             349..1863
                     /gene="caiT"
                     /locus_tag="ACHKW0_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiporter"
                     /protein_id="HFO7475888.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGCNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1894..3036
                     /gene="caiA"
                     /locus_tag="ACHKW0_003097"
     CDS             1894..3036
                     /gene="caiA"
                     /locus_tag="ACHKW0_003097"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HFO7475889.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            3165..4382
                     /gene="caiB"
                     /locus_tag="ACHKW0_003098"
     CDS             3165..4382
                     /gene="caiB"
                     /locus_tag="ACHKW0_003098"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HFO7475890.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4456..5996
                     /gene="caiC"
                     /locus_tag="ACHKW0_003099"
                     /pseudo
     CDS             4456..5996
                     /gene="caiC"
                     /locus_tag="ACHKW0_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA];
                     GO_process: GO:0009437 - carnitine metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
     gene            6105..6890
                     /gene="caiD"
                     /locus_tag="ACHKW0_003100"
     CDS             6105..6890
                     /gene="caiD"
                     /locus_tag="ACHKW0_003100"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HFO7475891.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGTEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAVEGPLAFAEKRDPVWKGR"
     gene            6896..7486
                     /gene="caiE"
                     /locus_tag="ACHKW0_003101"
     CDS             6896..7486
                     /gene="caiE"
                     /locus_tag="ACHKW0_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HFO7475892.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7572..7967)
                     /gene="caiF"
                     /locus_tag="ACHKW0_003102"
     CDS             complement(7572..7967)
                     /gene="caiF"
                     /locus_tag="ACHKW0_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HFO7475893.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(8228..11449)
                     /gene="carB"
                     /locus_tag="ACHKW0_003103"
     CDS             complement(8228..11449)
                     /gene="carB"
                     /locus_tag="ACHKW0_003103"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HFO7475894.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11467..12615)
                     /gene="carA"
                     /locus_tag="ACHKW0_003104"
     CDS             complement(11467..12615)
                     /gene="carA"
                     /locus_tag="ACHKW0_003104"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HFO7475895.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
     gene            complement(13071..13892)
                     /gene="dapB"
                     /locus_tag="ACHKW0_003105"
     CDS             complement(13071..13892)
                     /gene="dapB"
                     /locus_tag="ACHKW0_003105"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HFO7475896.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESDLFDMRDVLDLNSL"
     gene            complement(14059..14973)
                     /gene="rihC"
                     /locus_tag="ACHKW0_003106"
     CDS             complement(14059..14973)
                     /gene="rihC"
                     /locus_tag="ACHKW0_003106"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HFO7475897.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLDVKGFQQWVAEVLALAS"
     gene            complement(15039..15989)
                     /gene="ispH"
                     /locus_tag="ACHKW0_003107"
                     /gene_synonym="lytB"
     CDS             complement(15039..15989)
                     /gene="ispH"
                     /locus_tag="ACHKW0_003107"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HFO7475898.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(15991..16440)
                     /gene="fkpB"
                     /locus_tag="ACHKW0_003108"
     CDS             complement(15991..16440)
                     /gene="fkpB"
                     /locus_tag="ACHKW0_003108"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7475899.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(16528..17022)
                     /gene="lspA"
                     /locus_tag="ACHKW0_003109"
     CDS             complement(16528..17022)
                     /gene="lspA"
                     /locus_tag="ACHKW0_003109"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HFO7475900.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(17022..19838)
                     /gene="ileS"
                     /locus_tag="ACHKW0_003110"
     CDS             complement(17022..19838)
                     /gene="ileS"
                     /locus_tag="ACHKW0_003110"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HFO7475901.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(19881..20822)
                     /gene="ribF"
                     /locus_tag="ACHKW0_003111"
     CDS             complement(19881..20822)
                     /gene="ribF"
                     /locus_tag="ACHKW0_003111"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HFO7475902.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLALGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(20830..21048)
                     /gene="yaaY"
                     /locus_tag="ACHKW0_003112"
     CDS             complement(20830..21048)
                     /gene="yaaY"
                     /locus_tag="ACHKW0_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HFO7475903.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            21151..21414
                     /gene="rpsT"
                     /locus_tag="ACHKW0_003113"
     CDS             21151..21414
                     /gene="rpsT"
                     /locus_tag="ACHKW0_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HFO7475904.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT         5200 a         5820 c         5559 g         5136 t
ORIGIN      
        1 ccaactttca atattgttaa gttcctcacc aatattgaaa acatggcgta gcaaaaagaa
       61 attttcaata ttgctttatg gatcaccaat attgaaagtc agatgctatt tttttactca
      121 caacacaaca taacaaactg attaataatc aaaattaacg agaaaagcgc cctctattca
      181 cccgaaacaa aaatgtgata acaatcacag aatgcagctt attgaatacc cattatgagt
      241 tagccattaa cgcgtccacg aggttaataa taattatatt aaatgttaac aaaaataaaa
      301 caaacgggaa cgcaaaataa atattcgttt tcacagtgga attaactcat gaagaatgaa
      361 aagagaaaaa cgggaataga accgaaggtt ttctttccgc cgttaataat cgtcggcata
      421 ctttgttggc ttacagtcag agatctggat gcagcgaatg tcgttattaa tgctgtattc
      481 agttacgtca ccaatgtatg gggatgggca tttgaatggt atatggtggt gatgcttttc
      541 ggttggttct ggctggtgtt tggcccgtat gccaaaaagc gtttaggtaa cgaaccacct
      601 gaatttagta ccgccagttg gatctttatg atgttcgcct cctgtacgtc tgctgccgta
      661 ctgttctggg gatcgattga gatctactac tacatctcca ccccgccgtt tggcttagaa
      721 ccgaactcga caggggcgaa agagttgggg ctggcttaca gcttgttcca ctggggacct
      781 ctgccgtggg ccacttacag cttcctttca gtcgccttcg cttacttctt ctttgtccgc
      841 aaaatggaag tgattcgccc cagctcgaca ctggtgccgc tggtaggtga aaaacacgcc
      901 aaagggttgt tcggcactat cgtcgacaac ttctatctcg tcgccttgat cttcgcgatg
      961 ggtaccagtc tgggccttgc cacgccgctg gtgaccgagt gtatgcaatg gttgtttggc
     1021 attccgcata ccctgcaact ggacgctatc atcattacct gctggattat cctcaacgcc
     1081 atttgcgtcg cttgcggtct gcaaaaaggg gtacgtatcg ccagtgacgt gcgtagttac
     1141 ctgagcttcc tgatgctggg ttgggtgttc attgtcagcg gtgccagctt catcatgaac
     1201 tacttcaccg attcggtggg gatgttgctg atgtatctgc cgcgcatgtt gttctatacc
     1261 gatcccatcg ctaaaggcgg cttcccgcag ggctggaccg tgttctactg ggcatggtgg
     1321 gtgatttatg ctatccagat gagtatcttc ctcgcccgca tctcccgtgg tcgtactgtg
     1381 cgtgaactgt gcttcggcat ggtgctgggg ctgacagcgt caacctggat cctgtggact
     1441 gtactcggtt gtaacactct gctgttgata gataaaaaca ttatcaacat tccaaatctg
     1501 atcgaacagt acggtgtggc gcgcgccatt atcgaaacct gggccgctct gccactcagc
     1561 accgccacca tgtggggctt cttcatcctc tgctttattg ccaccgttac gctggttaac
     1621 gcctgctctt ataccctggc gatgtccact tgccgcgaag tacgcgatgg tgaagaacca
     1681 ccgctgctgg tgcgtatcgg ttggtcaatt ctggttggca ttatcggtat tgttttgctg
     1741 gcgctcggcg gcctgaaacc gattcaaact gccattatcg ccggaggatg cccgctgttc
     1801 ttcgtcaaca ttatggtgac gctctccttt attaaagacg cgaaacagaa ctggaaagat
     1861 taattaaccc ccaaaatatc aagaggttga aagatggatt ttaatttaaa tgatgagcag
     1921 gaactgtttg tcgccggtat ccgcgaactg atggccagcg aaaactggga ggcctatttt
     1981 gccgagtgcg accgtgacag cgtctacccg gaacgttttg tcaaagcact ggcggatatg
     2041 ggtatcgaca gtctgctgat ccctgaagag cacggtggtc tggacgcggg gtttgttact
     2101 ctcgccgccg tgtggatgga gctgggacgt ctgggggcac caacctatgt actgtaccag
     2161 ttgccgggcg ggttcaacac cttcctgcgc gaaggcacac aagagcagat cgacaagatt
     2221 atggctttcc gcggcaccgg taagcagatg tggaactcag cgattaccga accgggcgcg
     2281 ggctccgacg tgggtagcct gaaaacgact tatacccgta gaaatggtaa gatttatctt
     2341 aatggtagta agtgttttat taccagcagc gcctacaccc cgtacatcgt ggtgatggcg
     2401 cgcgacgggg cttctccgga caaacctgtc tacaccgaat ggtttgttga tatgagcaaa
     2461 ccgggcatca aagtgaccaa acttgaaaag ctcggtctgc gtatggatag ctgctgtgaa
     2521 atcacctttg acgacgtgga actggacgag aaagacatgt tcggtcggga aggtaacggc
     2581 tttaaccgcg tcaaagaaga gttcgaccat gaacgtttcc tggtagccct gaccaactac
     2641 ggtacggcga tgtgcgcctt tgaagatgcg gcgcgctacg ccaaccagcg cgtgcagttt
     2701 ggcgaggcta ttggtcgttt ccagttgatt caggaaaaat tcgcccacat ggcgatcaaa
     2761 ttaaactcca tgaaaaacat gctgtatgaa gcagcgtgga aagcagacaa cggcaccatc
     2821 acctctggcg atgcagcgat gtgcaaatac ttctgcgcca atgcggcatt tgaagtggtg
     2881 gatagcgcaa tgcaggtgct gggcggtgtc gggattgcgg gcaaccaccg catcagccgc
     2941 ttctggcgtg acctgcgtgt agaccgcgtc tccgggggat ctgacgaaat gcagatcctg
     3001 acgctgggtc gtgcggtgct gaagcaatac cgctaagtca atgcgctgcc tgatgcgacg
     3061 ctgatgcgtc ttatcaggcc tacgggagca cgaatgtagg ccggataaag cgtttacgcc
     3121 gcatccggca gtcatgcgct acataaattt ttcaggagca aataatggat catctaccca
     3181 tgccgaaatt cgggccgttg gccggattgc gcgttgtctt ctccggtatc gaaatcgccg
     3241 gaccgtttgc cgggcaaatg ttcgcagaat ggggcgcgga agttatctgg atcgagaacg
     3301 tcgcctgggc cgacaccatt cgcgttcaac cgaactaccc gcaactctcc cgccgcaatt
     3361 tgcacgcgct gtcgttaaat attttcaaag atgaaggccg cgaagcgttt ctgaaattaa
     3421 tggaaaccac cgatatcttc atcgaagcca gtaaaggtcc ggcctttgcc cgtcgtggca
     3481 ttaccgatga agtactgtgg cagcacaacc cgaaactggt tatcgctcac ctgtccggtt
     3541 ttggtcagta cggcaccgag gagtacacca atcttccggc ctataacact atcgcccagg
     3601 cctttagtgg ttacctgatt cagaacggtg atgttgacca gccaatgcct gccttcccgt
     3661 ataccgccga ttacttttct ggcctgaccg ccaccacggc ggcgctggca gcactgcata
     3721 aagtgcgtga aaccggtaaa ggcgaaagta tcgacatcgc catgtatgaa gtgatgctgc
     3781 gtatgggcca gtacttcatg atggattact tcaacggcgg cgaaatgtgc ccgcgcatga
     3841 gcaaaggtaa agatccctac tacgccggtt gcggtctgta taaatgcgcc gacggctaca
     3901 tcgtgatgga actggtgggc attacccaaa ttgaagagtg ctttaaagat attggcctcg
     3961 cacatctact cggtacgccg gaaatcccgg aaggcactca gcttatccac cgtatcgaat
     4021 gcccttacgg cccactggta gaagaaaaac tcgatgcctg gctggcggca cataccatcg
     4081 cagaagttaa agaacgcttt gccgaactga atatcgcctg tgccaaagta ctgaccgtac
     4141 cggaactgga aagcaatcca cagtatgttg cccgtgaatc aatcactcag tggcaaacga
     4201 tggatggtcg cacctgcaaa gggccgaaca tcatgccgaa attcaaaaat aaccccggac
     4261 aaatctggcg cggaatgccc tcacatggca tggacacggc tgccattttg aaaaatatcg
     4321 gctacagcga aaacgacatt caggagttgg tcagcaaagg tctggccaaa gttgaggact
     4381 aaacgcgtgg cgtcagtcgg cttgcctgac tagcgcccgc ccaataacaa acgaacaaaa
     4441 atggatagag gtgcaatgga tatcattggc ggacaacatc tacgtcaaat gtgggacgat
     4501 cttgcggacg tttacggtca taaaacggcg ctgatttgtg aatccagcgg cggagtcgtt
     4561 aaccggtata gttatcttga gttaaatcag gagattaacc gcacggcaaa cctgttttat
     4621 acgctgggga ttcgcaaagg cgacaaggtt gcactacatc tcgacaactg cccggaattt
     4681 atcttttgct ggttcgggct ggcaaaaatt ggcgcgatta tggtgccgat taacgcccgc
     4741 ctgttacgcg aggaaagcgc gtggatcctg caaaatagcc aggcgtgcct gctggtgacc
     4801 agtgcgcaat tctatcctat gtatcaacag attcagcagg aagatgccac tcaattgcgg
     4861 cacatttgcc tgacagatgt ggcacttccc gctgatgatg gcgtgagttc gtttactcaa
     4921 ctgaaaaatc aacaacctgc caccttgtgc tatcgactga cgatacggcg gaaattctct
     4981 tcacctccgg caccacctcc cgaccgaaag gtgtggtgat tacccattac aacctgcgct
     5041 tcgctggata ttactccgcc tggcagtgtg cactgcgtga cgatgacgtc tacctgacgg
     5101 taatgcctgc gtttcatatc gattgccagt gtactgcggc gatggcggcg ttttctgccg
     5161 gggccacctt tgtgctggtc gagaaataca gcgcccgcgc cttctgggga caggtgcaga
     5221 agtaccgcgc caccattacc gaatgtattc cgatgatgat ccgtacgttg atggtgcagc
     5281 cgccttcagc gaacgatcgg caacaccgcc tgcgggaagt gatgttttat ctcaacttgt
     5341 cggagcagga aaaagatgcg ttttgtgaac gcttcggcgt tcgcttgctg acgtcttatg
     5401 ggatgacgga aaccattgtg ggcattatcg gcgatcgccc tggcgataaa cgacgctggc
     5461 cgtcgattgg tcgggcgggg ttttgctacg aagcggagat ccgcgacgat cacaatcgcc
     5521 cgctcccggc aggtgagatc ggtgaaatct gtattaaagg cgtaccaggg aaaaccatct
     5581 tcaaagagta ttttctcaac ccgaaagcca ctgcgaaagt gctggaagcc gacggctggc
     5641 tacataccgg cgataccgga tactgcgacg aagagggctt tttttatttc gtcgatcgcc
     5701 gctgcaatat gatcaaacgc ggtggcgaga atgtctcctg cgtggagctg gaaaatatca
     5761 tcgccaccca tccaaaaatt caggatatcg tggttgtggg tattaaagat tcgattcgcg
     5821 atgaagccat caaagcattt gtggtgctga atgaaggtga aacattgagc gaagaggaat
     5881 ttttccgctt ctgcgaacaa aatatggcga aatttaaagt gccctcttat ctggagatca
     5941 gaaaagatct gccacgtaat tgctcgggga aaataattag aaagaatctg aaataaatga
     6001 aacagcaggg aactaccctg ccagctaata atcacgctat aaaacaatat gcattttttg
     6061 cagggatgtt gtcgtccctg aaaaagcaaa aatggagaaa aggaatgagt gaatcattac
     6121 atctgacccg caatggatca attctggaaa ttacccttga tcgtccaaaa gcgaatgcta
     6181 ttgatgcaaa aaccagcttt gaaatgggcg aagtatttct aaatttccgt gacgatccgc
     6241 aattacgtgt cgccattatt accggtgccg gagagaagtt cttttccgcg ggctgggatt
     6301 taaaagcggc agcagaaggc gaagcaccgg atgctgactt tggcccgggt ggatttgcgg
     6361 gattaaccga aattttcaat ctcgacaaac cggttatcgc agctgtgaac ggctatgcct
     6421 ttggcggcgg ctttgaactg gcgctggcgg cagattttat tgtttgtgcc gataatgcca
     6481 gcttcgccct gccggaagcc aaactgggca tcgttcctga cagcggcggc gtgctgcgtc
     6541 tgccgaagat cctgccgcct gccatcgtca atgaaatggt gatgaccggc agacgaatgg
     6601 gcacagaaga ggcgctgcgt tgggggatag tcaaccgcgt ggttagccag gcggaactga
     6661 tggataacgc ccgcgaactg gcgcagcagc tggttaacag cgccccgctg gcgattgcgg
     6721 cgctgaaaga gatctaccgc accaccagcg aaatgccggt agaagaagcg tatcgctata
     6781 ttcgcagcgg cgtgttgaaa cactatccgt cggttctgca ttcggaagat gccgttgaag
     6841 ggccgctggc gtttgccgag aagcgcgatc cggtatggaa aggacgttaa caaccgtgag
     6901 ctattacgcc tttgaggggt taattccggt ggttcacccg acggcgtttg tccatcccag
     6961 tgccgtcttg attggcgatg tgattgtggg agccggtgtc tacatcggcc cactcgcctc
     7021 actgcgtggt gactacgggc ggttgatcgt gcaagcggga gccaatattc aggatggctg
     7081 cattatgcat ggctactgcg acactgacac catcgttggg gaaaacggcc atatcgggca
     7141 cggagcgatc ctgcatggtt gtgtgattgg tcgcgatgca ttggtcggga tgaacagcgt
     7201 gattatggat ggcgcggtca ttggcgaaga gagcattgtt gccgccatga gctttgtcaa
     7261 agcaggcttt cacggcgaga aacgccagtt gttgatgggt acgccagccc gcgctgtacg
     7321 cagtgttagt gacgacgagt tacactggaa acggttgaat accaaagagt atcaggatct
     7381 tgtcgggcgc tgtcatgcat cgttacatga aacacagcca ctgaggcaaa tggaggaaaa
     7441 tcgcccccgt ttgcagggga cgacggatgt gacgcctaaa cggtgatctt gccggatgcg
     7501 acctggttgc tggataagat gctacaacat cgcatctggc aagcaggacg cccggtatca
     7561 acgagataaa attaacgacg catactcttt gacaacatca tctgccactt ctgctcgcgg
     7621 ttaagttccg tgagcggaac ggcaggaata cgcggcgtct ttcttacgcc gactaattta
     7681 tcccggctgt tattgcgcag gcgcgcgtaa atttgctcat cgatatcgac cacttttacc
     7741 agtcgctggc actggcatcc ccgtccttcc agcttattag ggatcatctt aacttcgcag
     7801 cttatttctg cgacttccga cagaatataa gtcagggtat taaccgcctt gctgtgttca
     7861 atatcgaaat gaatagagat ctctcttgct atcacccacc gattttcagc catcatccat
     7921 tcggcgatta acaagtagag tggtttttca acatatcctt cacacatacg aactccagtt
     7981 tcacaaatta aaagtctggc tatgactcgt tttgaagcaa ttaaacaagg ccatattaaa
     8041 attgtggata tatcgatagt atgcaacttc accgtcgata agtgtgagct aaatcctgct
     8101 tatgtcatca aaaatatcga ttccccaaaa gatttactta atgggcaaaa aaaacggtcc
     8161 tcatcagaga accgtattac ttattcgatc aaattagcgg atgaaaaata tccgccatga
     8221 cacgctatta tttgatctgc gcgtgcattt cctgcaccga aattactttt tcggtcgcgt
     8281 cggcattcag cgccatcgcg gtagcgaaac caccgttcag ggtggtgtca taatgcactt
     8341 tatattgcag cgcactgcga cggatcacgc gggagtcttc aatcgcgcga cggcctgaag
     8401 tggtgttgat gatgtaggta tattcgccat tcttgatgcg gtcctgaatg tgcggacggc
     8461 cttcatgcac cttgttcacc agacgcggat taatgcccgc ttcgcccagc acaatcgccg
     8521 tgccgtgggt cgcatccagc tcgaagccct gtttcagcag ttttgccgcc aggtccacca
     8581 cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg accgtgtttc ttcatggtgg
     8641 agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa ggtgcggccc acgcccatca
     8701 cttccccggt ggagcgcatt tctggcccta acagcgggtc aacacccggg aatttattga
     8761 acggcagcac cacttctttc accgagtagt acggcgggat aacttctttg gttacgccct
     8821 gctcagccag cgatttgcca gccatcacac gcgccgccac ttttgccagc ggtacgccgg
     8881 tggctttgga gacgaacgga acggtacgcg ccgcacgcgg gttaacttca atcaggtaga
     8941 cttcgttgtt tttcaccgca aactgcacgt tcatcagacc gcgcacctgc aattcgaagg
     9001 ccagtttctg cacctgctgg cgcatcacat cctgaatttc ctgacttaag gtgtaggctg
     9061 gcagagaaca tgcggagtca ccggagtgca cgcccgcctg ctcgatgtgc tccatgatgc
     9121 cgccaatcag caccatttcg ccgtcgcaga tggcatccac gtcaacttct accgcgtcat
     9181 caaggaagtg atccagcaac actggcgcat cgttagacac gctgaccgcc gtctggaagt
     9241 agcgacgcag gtcagcttcg tcatagacga tttccatcgc ccgaccgccg agaacgtaag
     9301 acggacgtac caccagcggg tagccaatct ctttcgcctt ctctaccgcc atttcaatag
     9361 cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg ctcaaccgca tgctggaagc
     9421 gttcacggtc ttctgcacgg tcgatagcat ccgggctggt gccgataacc ggtacgccag
     9481 cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc gccgtactga acgataacgc
     9541 ctttcggctt ctcaatacgc acgatttcta gcacatcttc cagagttacc ggctcgaagt
     9601 agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc cgggttacag ttaaccataa
     9661 tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg tacacagcag tagtcgaatt
     9721 cgataccctg accgatacgg ttcgggccgc cgccgagaac catgattttt tcacggtcgg
     9781 tagacggatt cgcttcgcac tcttcttcat aagtggagta catgtaagcg gtgtcggtgg
     9841 cgaactctgc cgcacaggta tccacgcgct tataaaccgg gtgcaggtca tactggtcgc
     9901 gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt tgccagacgc gcatcggcaa
     9961 agcctttgcg tttcagctgg cgcaggaatt cagcgttcag gccagtgatg cccacttccg
    10021 ctactttctc ttccagacgc accagttctt caatctgtac caggaaccag cggtcaatat
    10081 tggtcaggtt gaacacgccg tccacagaca ggcccgcacg gaaggcatcg gcgatgtacc
    10141 agatacgctc tgcgccagca tctttcagtt cgcgacggat tttggttaac gcttccgggt
    10201 catcgaggct cactttcggg tcgaatccag tcgcaccgac ttccaggccg cgcagcgctt
    10261 tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac ttcgccaacc gatttcatct
    10321 gagtggtcag acggtcgtta gcaccagcga atttttcgaa gttgaagcga ggaattttag
    10381 taaccacata gtcgatggac ggctcgaagg aggccggagt acgtccgcca gtgatgtcgt
    10441 tcatcagttc gtcgagggtg taacccaccg ccagtttcgc cgccacttta gcaatcggga
    10501 aaccggtcgc tttcgacgcc agcgccgaag aacgggacac gcgtgggttc atttcgataa
    10561 caatcaggcg accgtttttc gggttcaccg caaactggac attggaacca ccggtttcaa
    10621 cgccgatttc acgcagcacc gccatcgagg cgttacgcat gatttgatat tctttgtcgg
    10681 tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt atggatgccc atcgcatcaa
    10741 agttttcgat agagcagacg atgatgcagt tgtcgttttt atcacgcacc acttccatct
    10801 cgtactcttt ccagccgatc agcgactcat caatcagcag ctctttggtt ggggagagat
    10861 ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt ataagcgata ccgccgccgc
    10921 taccgcccat ggtaaaggat gggcgaataa tgcacgggaa gcccacgtca gcggcaaccg
    10981 ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc ggtttccaga ccaattttct
    11041 tcatcgctac gtcgaaacga cggcggtctt ctgctttatc aatcgcatcg gcggtcgcac
    11101 caatcatagt cacgccgaac tcttccaaca cgccctgacg ctccagctcc agcgcgcagt
    11161 tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc cgggcgctct ttttcaataa
    11221 tcttgcgtac tacttcccag tgaatcggct cgatgtaggt cgcatcggcc atttccgggt
    11281 cggtcatgat ggtcgccggg ttggagttca ccagaatgac gcggtaaccc tcttcgcgca
    11341 gggctttaca tgcttgcgcg ccagagtagt caaactcaca cgcctgaccg ataacaatcg
    11401 ggcccgcacc cagaatcagg atacttttta tatctgtacg ttttggcatg gctcttttac
    11461 tcctgattac ttagcggttt tacggtactg ctcaattaac tcgataaagt ggtcgaacaa
    11521 cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga ccctggaagc tgaacgccgg
    11581 tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac agggatttat gcgtgacacg
    11641 caggtttgca ggtaatgttg cttcatccac cgcaaaaccg tggttctggg cggtgatcat
    11701 caccacgttt ttctccacat ctttaaccgg atggttgccg ccgtggtgac caaatttcat
    11761 tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga tgaccgaggc agatgccgaa
    11821 taccggaata tcggtttcga ggaatttctg gatggcggta atggcgtaat cgcacggggc
    11881 cgggtcgcca ggaccgttgg agaggaagat gccgtctgga ttcattttca gcacatcttc
    11941 cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct ctatccacca gcatccgcag
    12001 gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg aacggcagct cgtcttcttt
    12061 tttcgcttct ggcaggccgc cggtcaacgt ccagctccct tgtgtccagc tataggcttc
    12121 tgcggtggtc acttcttttg ccagatccat gccattcaga cctgggaacg cgcgggcttt
    12181 ttctaacgcc aacgccgcat ccgggttatc gcctgcgata atgcagccat tctgtgcgcc
    12241 tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata tcggcaatcg ccacgatgtt
    12301 atggcgcttc aggtaagaag agaggtcttc ggtattacgg aagttgctgg caatcagcgg
    12361 cagatcgcga atcaccagac cttgtgcatg tacctgagaa gattcttcat cggcgtcatt
    12421 agtgccgaca ttgccaatat ggggataagt aagagtaacg atttgacgag aataggaagg
    12481 atcagtgagg atttcttgat aaccggtcat tgaagtattg aaaacgactt ccccaaccgc
    12541 cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt ccgtcttcca gaaccaatag
    12601 cgctgactta atcaaaacac cctccagaga atattcactc actttatttg catattaatt
    12661 cacaatgatg acgtaaatca atgcaaatct gcttaccgac gaatttctgg caaacggcgg
    12721 cattctggag atatgggcgc taaaagtcaa cttaatggcg ggatttttta tattattttg
    12781 tggctcatgc atgttttaca ggcattaagc gacaaaaaga tcatataacc tgttctggaa
    12841 aacgcttgcg cagcaagaag attgcatgaa ataaaagcga gcagggaaaa gtggaccaaa
    12901 tggtcaaaat ttacattaaa acaacaaaaa ccgaagacaa aacatgtaat ttttggcact
    12961 tatagacaat ttaaaaacat aacattaaaa ataaaagggc caaaaattaa agccctttat
    13021 aatcaataaa ttaaatgtgt tatttttgca ccataacaaa tattttgtgg ttacaaacta
    13081 ttgagatcaa gcacatctcg catatcaaaa agatcacttt ccttaccact caaccacaaa
    13141 gccgatctta ccgcgccgtt agcaaatgtc atgcggctgg acgccttatg agtgatctcc
    13201 agacgctcgc caatatcggc aaacatcgcg gtatgttcac caacgatgtc acctgcacgc
    13261 acggtggcaa aaccaatggt gccaggcaca cgttcaccgg tgtggccttc acgactgtag
    13321 accgcgcaat ctttcagatc tttatcaagg gcgtgggcga tcgcctctcc cattgccagt
    13381 gcggtgcctg acggcgcatc aactttatgt ctatgatgtg cttcaataat ttcgatatcg
    13441 gtgtagtcac ccatcacttt ggctgctttc tccagcagct taagcatgac gttaacgcca
    13501 acgctaaaat tggcggcaaa gacaatcgca atatcggcgg cggcgtcacg aatcgcctgt
    13561 ttaccggctt cgtcaaaccc cgtagtgccg atcaccatcc ctttgccatg ctggcgacaa
    13621 aaagcgagat ggttcagcgt accttccgga cgggtaaaat cgataaacac atcaaaatca
    13681 tcttttaccg catcgaggct gctttgcacg gtaacgcctg ttttccctgc accagccagc
    13741 tcaccggcgt cgctgcccag taaagaagat ccttcgcgct ccagcgccgc gcccagctgc
    13801 acgccttcta atgccagcgc cgcctgaatc aactggcggc ccatacgccc cccggctccc
    13861 gcgatggcaa cgcggatgtt tgcatcatgc atagctattc tcttttgtta atttgcatag
    13921 accgttttca gagtaaccag cgcatcgcta cgccgccagc caaaaacgct gataattaga
    13981 aattaatgat aaacagggag ggatatcagt gaaaggcatg agtcgatgaa tgactgcatg
    14041 ccaataacat gtgacaggtt acgacgccag agccagcacc tcagccaccc actgctggaa
    14101 gcctttcaca tccagatcca atgccacctg tacattggct ggcttgccca ggcaaccgtc
    14161 gatatcaacc accgtcgtgc ctgaggtaaa ttcgccctga gtttccactg ccacaaaaca
    14221 gggtttgaga gtgaacaggt ccgggcgcac cagccaggcg atggcgcaga gatcgtgcat
    14281 tcgcagaccg ctttgcatac tgccgctacg gtagtggcta aacagtgcat gaagcatttt
    14341 tccggtgcga ttcaactccg gcaaagtagc gagatagtca ggggttaata ttgcctgatt
    14401 ggtgacatcc agaccgcaca tgacgatttc gattccgcta cggaagacac aggcagccgc
    14461 ttctggatcg gcagcgatat taaactcggc gtttggcgta cagttgccgc gtccggcaga
    14521 accacccatg atcaccagac ggcgaatata cggcttgcat tccgggcatt gtgaaagtaa
    14581 cagcgcaata ttggttaacg ggccgatggc caccagggta acaggctctg gtgcacgcat
    14641 cagggcatcc cgaatcgcca gaaacgccgg tatcccgagc ggctttcggt tgtgctcaac
    14701 aaagtcgtag ccagccattc ccgattcgcc gtgcacagat gccgcatcac gcggtgcgcg
    14761 taccagcggc acagcggccc cttgggcgag cggaatctcc gcattccaga aatgcagcag
    14821 ttgcaaggca ttacgggtag ttttctcaac cgagacatta cccgcgacgg tggtcatcag
    14881 ttgcaggtcg agttcggggg caaaaatcgc ggcggcaatg gcgacggcat cgtcaatgcc
    14941 ggggtcagta tcgaggaaga taggtaaacg catgttttct ccataaaaaa tgccggtaac
    15001 aagaccggca ttttcgcata acttaggctg ctaatgactt aatcgacttc acgaatatcg
    15061 acacgcagct ctttcggcac ttcgaaaaca atgttttctt cacggccttc cagcggaatg
    15121 gcttcaccac cgcccagctg ctgcaaacgt gccaccacat tctgcaccag aatatccgga
    15181 gccgatgcgc ccgcagtcac gccgacgcat ttaacctctt tcacccactc ttcctggatg
    15241 tctttcgcat cgtcaatcaa aaacgcgcgt ttgcccatac gctgggccag ctccgccaga
    15301 cggttggagt tggaggagtt tttcgaaccg accaccaaca caacttccgc ctgttctgcc
    15361 agggcgcgta ccgcttcctg acggttagtc gtggcgtagc agatgtcatc tttgcgcgga
    15421 ccgacaattt tcgggaagcg tttacgcagc gcgtcgatca catcagacgt gtcatccacc
    15481 gacagcgtgg tctgggtcat aaaggagagc ttctcttcgt ttttgaccgt cagtttccac
    15541 acatcgtccg gcgattcgac cagatacatt cccccttccg ggttactgta ctggcccatt
    15601 gtcccttcca cttccgggtg cccggcgtga ccgatgagaa tagattcttc gccacggcga
    15661 ctggcgcggg cgacttccat atgcactttg gtcaccagcg gacaggtcgc gtcaaatacc
    15721 gtcaaatcgc gactttttgc ttcgttacgt accgcctgag aaacaccgtg tgcggagaaa
    15781 atcaggatcg cgccgtccgg tacttcgcta atctgctcaa taaagatagc cccacgctca
    15841 cgcaagctat cgaccacata gcggttatgt accacttcgt gacggacata tatcggtgcg
    15901 ccgtaaatgg ccagcgcgtt ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa
    15961 ccacgcgggt tggccaacag gatctgcatg ttacgcctcc agtgccggat cgatttccag
    16021 cacttcaata tcaaaatgaa cggtctgccc ggccagcgga tggttgaaat caacggtaat
    16081 ggagtcgccg ttaatttcgc ggatcacgcc aggcatctca ctgccatcca ttgcggtaaa
    16141 aagcatgatt gcgccaattt ctggctcgcc tgcatccata aattcacggc gggagaagta
    16201 ctgaatcagg tccggtgacg gcacgccaaa cgccgcatct ggctccaacg agaaggtggt
    16261 tttatcgccc actttcagcc ccaacaggtg ttgctccagc ccttcagaaa gagaagcatc
    16321 acccaggcgg aacagcgccg gtttaccgtt gttgcgggta gactcggcgg tggtgccatc
    16381 gtcgagtttt agcgtgaagt gcaccaggac ggcgctattg ctctgtacag attcagacat
    16441 gcaggtttgc tcttatattt tgaggatttt tgccggatgc ggcctacgat ttcgcagcaa
    16501 tctgtaggcc ttatccggca gggtttatta ttgttttttc gctttagaag gcaaaaaacc
    16561 ttccagcaca atcagtgccg caccgacaca aatggcagta tcggcaaggt tgaaggtggc
    16621 gaagtgccag tcgccgacgt agaagtcgat catatcgaca acgaagccgt gccacaggcg
    16681 gtcgaacagg ttgcccagcg cgccgccaat aatcagcgca taagcgatat tgtttagctt
    16741 ctgcgtggcc ttcgagcgat acatcatcac tgccaggatc acgctaatac caatcgcaat
    16801 accggcaaag aaccaacgct gccagccgcc gctatcggca aggaaactaa acgccgcgcc
    16861 atagttacgc gcataatgca gattaagcga cgggaacagc gggaccgtat cccccagagc
    16921 aaagttctgg aggatcaggt atttgctgcc cagatcgata atcagcacga ctaccaccag
    16981 ccacagccag cgtagccctg ttgaacagat cgattgactc atcaggcaaa cttacgtttt
    17041 tcaccgtcac cggcgacgtt gctgacacag cggccgcaga tttctgcgtg ttccgccacc
    17101 ttgccgacat cctgggtgta gtgccagcag cgtgggcact tctcaccttc ggctttactc
    17161 aacgcgactt tcagcccttt gagtacttcg ctctgctgag catcagcagg tgcgtcgtta
    17221 tagtctgcaa cggtagcgcc ggaggtcaac aggacaaatc gtaattcatc gcccagcgcg
    17281 gtcagtttcg ccgacagttc cggttctgca tacaaggtta ctgccgcttc cagcgagcca
    17341 cccactttct tgtcggcacg cgcttgctca atgactttgt tcacttcgcc acgcactttc
    17401 aacagctcgt cccagaacgc atcgttcatc gcttcactgt ctgccaggcc aaacaggcct
    17461 tcgtaccact caccggtgaa gacgtatttt tcacgttcgc ccggcaggta gccccacact
    17521 tcatcagcgg tgaaggagag gattggtgcc atccagcgca ccagcgcttc tgcgatgtga
    17581 tacagcgcag tctggcagct acgacgcgcc acactgtccg ctttggcggt gtactgacgg
    17641 tctttgatga tgtcgaggta gaaggaaccc atctcaacgg agcagaagcg catcagacgc
    17701 tgtaccactt cgtggaaatc gtatgcttcg tacgccttga ggatgtcttc ctgtgccgct
    17761 ttcgcacaac ctacggccca gcgatccagt accaccatct cttccggttt caccatatct
    17821 tttgctggat caaaaccgtt caggtttgcc agcaggaagc gcgcggtgtt acggatacga
    17881 cgatagctat cggcagcacg tttcaggatc tcgtcagaaa cggccatttc accggtgtag
    17941 tcggttgatg ccacccacag tcgcagaata tccgcgccca gtttgttcat cacatcctgc
    18001 ggcgaaacgg tattgccgat ggatttagac atcttgcgac cctgaccatc cacggtaaag
    18061 ccgtgggtca gtacctgacg atacggcgct ttgcctttca tcgcggtgga gatcattagg
    18121 gaagacatga accaaccacg gtgctggtca gaaccttcca gatacatgtc cgctgcgtga
    18181 ccggcaaatt ccggacgcac gtcaacaaca gaagagtggg tagatccgga gtcaaaccat
    18241 acatccaatg tgtccggcac tttcacgtac tgatcagctt catcgccgag gatctctttc
    18301 gcatcaagat cccaccacgc ctggatgccg tcaacttcaa cgcgttttgc cacttcttcc
    18361 attagttcga gtgtacgcgg atgcagctct tccgtgtctt tgtgcacgaa cagtgacatc
    18421 ggtacgcccc aggtgcgctg acgggagata caccagtcag gacggttagc aaccatcgac
    18481 tcgatacgcg cctggcccca gtccgggatc cactgcacgc ctttgatctc tttcagtgac
    18541 tgcgcacgca gacctttctg atccatgctg acgaaccact gcggcgtcgc gcggaagatg
    18601 atcggcgttt tgtgacgcca gcagcacgga tagctgtgct gcattttctc aacgtgcagc
    18661 agcgcgcctt tttcctgcag cagcgcaacg acgatgtcgt tcgctttgaa gacgttcacg
    18721 ccatccagcg tcggataagt gcccggcaga taagtgccgt ccgggccaac cgggttagcg
    18781 gtttccaggc cgtatttctg accgatcaca tagtcgtccg ggccgtggcc aggcgcggtg
    18841 tgaacggcac cggtaccggc atccagggta acgtgatcgc cgaggattgc cggaacgtcg
    18901 aagcccataa acggatgggt aaagcgcagc aactcaagct ccgcaccttt taccgtgccg
    18961 agaatggtgt aatcggtcac gccgatacgc tgcattacgc tttcaaccag atctttcgcc
    19021 agaatcacgg cctgaccgtc gatctgcacc agcgcatagt cgaaatcagg tgcaatagag
    19081 attgcgcggt tcgcaggcag agtccacggc gtagtggtcc agatcaccag cgagattggg
    19141 ccgttaacgt tgcttacggc aaattttgct ttcagtgcat cctgatcgac cgcctgaaaa
    19201 gcaacgtcga tggacggaga agttttgtcg taatactcaa cttccgcttc cgccagcgca
    19261 gaacggcagt caacgcacca gtgaactggc ttcgcgcctt tgtgcaggtg accgttgccg
    19321 atgattttgc ccagcgcgcg gatgatgttg gcttcagttt tgaagtccat ggtcaggtac
    19381 gggtgcgacc agtcgcccag cacgcccaga cggataaagt ctttgcgttg accgtcaacc
    19441 tgggtcgccg cgtattcgcg gcacttggcg cggaactcgg cggcggtgaa tttctcaccc
    19501 ggcttaccgt attcttgctc tactttcagc tcgatcggca gaccgtggca gtcccagcca
    19561 ggcacatacg gcgagtcata accggaaagc cctttggact tcacgataat gtctttcaga
    19621 atcttgttaa ccgagtgacc aatatgaatg ctgccattcg cataaggagg gccatcatgc
    19681 agaatgaagg tttttttgcc ttttttagcc gcacggatga tgccgtacag atcatcatca
    19741 gtccaacgcg ccagcattcc cggttcgcgc ttggcgagat cgccacgcat cgggaaccct
    19801 gtttccggca aattcagggt tgatttatag tcactcatca gattctcggt tccgtgtttc
    19861 ggtttgatta cataacaggc ttaagccggt tttgttagcc caaaaaattc gcgggcggtt
    19921 aattcatcac gcgcaatctg cgctttcagt tcgtccagcg acgcaaatcg ctgctcattg
    19981 cgtatttttt tacgcagcac tacttgtata tggcgaccgt aaaggtccat tgcaacatct
    20041 aacaaatgca cttccagctg ctggcgaata ccggcaaccg ttgggcgtgt tccaatgttt
    20101 gccacgccgg gtaacggctt ttcaccgagg cccagcactt ctaccgcata aacccctttc
    20161 accggggaaa cctgacggcg tagcggtaca ttcgccgtcg ggaaacctat agtgcgccct
    20221 aattcatcac cgtggactac acgcccggag atggcaaacg ggtgccccag taaactttct
    20281 gccagagcca gattgtcatc cgccagcgcc tgacgcacag cggtgctgct gatacgcacg
    20341 ccaccttcgc aaaaagtttg cgtactggtg atatcgaagc cgtattccat gccagctttc
    20401 tgtaataaca agaaatcgcc ttcacgacca gcgccaaagc ggaaatcatc acctaaggca
    20461 agaaatttta cgcgcaaatg cttcaccaga agatcgctga tgaaattttg cgcggttaac
    20521 gccgcgaaac gcctgtcgaa acgcacgcac agcacgtaat caacgccaca ctctgcaagg
    20581 taacgcagtt tttcccgcag ccgggtcagc cgtgccgggg ctttatcggt agcaaacagt
    20641 tccagtggtt gaggttcaaa aagcatcacc atcaccggta agttgcgctt gcgcccttct
    20701 tcctgcaagc cctgtaacag cgcgcgatga ccgcgatgca cgccgtcgaa attaccaata
    20761 gtcagcacac acccttcttg cggggcctgg ctgagattat gtatgccgcg tatcagcttc
    20821 atgtctggct caaaacagtg aaaatcgtcc gagtatacct tgtacagcgg tcaaggttaa
    20881 ccggcgattg agtaccgaaa tcagaaagaa gtggcttttc attgccatgg cgcaaatcac
    20941 gggaagaaag tgaccgcctg ctgcaatttt tatcgcggaa aagctgtatt cacaccccgc
    21001 aagctggtag aatcctgcgc catcactacg taacgagtgc cggcacatta acggcgctta
    21061 tttgcacaaa tccattgaca aaagaaggct aaaagggcat attcctcggc ctttgaattg
    21121 tccatataga acacatttgg gagttggacc ttggctaata tcaaatcagc taagaagcgc
    21181 gccattcagt ctgaaaaggc tcgtaagcac aacgcaagcc gtcgctctat gatgcgtact
    21241 ttcatcaaga aagtatacgc agctatcgaa gctggcgaca aagctgctgc acagaaagca
    21301 tttaacgaaa tgcaaccgat cgtggaccgt caggctgcta aaggtctgat ccacaaaaac
    21361 aaagctgcac gtcataaggc taacctgact gcacagatca acaaactggc ttaatcgcca
    21421 atttgctgaa gctttgtgaa aaagcccgcg caagcgggtt tttttatgcc tgctgctttt
    21481 atgatgcgtg agagaacaca ctaccggttg ccagatagtc aaaagatgct ggcttttgca
    21541 gacatgccgt tacactttct gaactcttcg tggcttaaga atcgtagaac catcggctga
    21601 tacattctga acaagaatat ctactcaatc tccagactcc cctacaaaga atgtggatat
    21661 tgtgatacac attgaggtat taaacttaag accactctat ttttgaatta aattc
//
LOCUS       DAZFXT010000077        21525 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.077, whole genome shotgun sequence.
ACCESSION   DAZFXT010000077 DAZFXT010000000
VERSION     DAZFXT010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21525
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.077"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..373
                     /locus_tag="ACHKW0_003114"
     CDS             <1..373
                     /locus_tag="ACHKW0_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7475905.1"
                     /translation="LIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFTKEYRIS
                     PINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPSDYRRQF
                     KNCFAEQEILSEFPQPVSLAG"
     gene            complement(416..1567)
                     /gene="ydiO"
                     /locus_tag="ACHKW0_003115"
     CDS             complement(416..1567)
                     /gene="ydiO"
                     /locus_tag="ACHKW0_003115"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HFO7475906.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(1581..3176)
                     /gene="ydiF"
                     /locus_tag="ACHKW0_003116"
     CDS             complement(1581..3176)
                     /gene="ydiF"
                     /locus_tag="ACHKW0_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HFO7475907.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(3320..4078)
                     /gene="aroD"
                     /locus_tag="ACHKW0_003117"
     CDS             complement(3320..4078)
                     /gene="aroD"
                     /locus_tag="ACHKW0_003117"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HFO7475908.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(4109..4975)
                     /gene="ydiB"
                     /locus_tag="ACHKW0_003118"
     CDS             complement(4109..4975)
                     /gene="ydiB"
                     /locus_tag="ACHKW0_003118"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HFO7475909.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(4987..6252)
                     /gene="ydiN"
                     /locus_tag="ACHKW0_003119"
     CDS             complement(4987..6252)
                     /gene="ydiN"
                     /locus_tag="ACHKW0_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7475910.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(6479..7693)
                     /gene="ydiM"
                     /locus_tag="ACHKW0_003120"
     CDS             complement(6479..7693)
                     /gene="ydiM"
                     /locus_tag="ACHKW0_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7475911.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILHTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFFSFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(7793..8149)
                     /gene="ydiL"
                     /locus_tag="ACHKW0_003121"
     CDS             complement(7793..8149)
                     /gene="ydiL"
                     /locus_tag="ACHKW0_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HFO7475912.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(8285..8392)
                     /gene="rprA"
                     /locus_tag="ACHKW0_003122"
     ncRNA           complement(8285..8392)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="ACHKW0_003122"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(8578..9690)
                     /gene="ydiK"
                     /locus_tag="ACHKW0_003123"
     CDS             complement(8578..9690)
                     /gene="ydiK"
                     /locus_tag="ACHKW0_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HFO7475913.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            10079..13135
                     /gene="ydiJ"
                     /locus_tag="ACHKW0_003124"
     CDS             10079..13135
                     /gene="ydiJ"
                     /locus_tag="ACHKW0_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding and (Fe-S)-binding domain-containing
                     protein"
                     /protein_id="HFO7475914.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIVSQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQISI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHKNSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            13132..13542
                     /gene="menI"
                     /locus_tag="ACHKW0_003125"
     CDS             13132..13542
                     /gene="menI"
                     /locus_tag="ACHKW0_003125"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HFO7475915.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            13642..13830
                     /gene="ydiH"
                     /locus_tag="ACHKW0_003126"
     CDS             13642..13830
                     /gene="ydiH"
                     /locus_tag="ACHKW0_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HFO7475916.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            14378..14746
                     /gene="sufA"
                     /locus_tag="ACHKW0_003127"
     CDS             14378..14746
                     /gene="sufA"
                     /locus_tag="ACHKW0_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HFO7475917.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            14755..16242
                     /gene="sufB"
                     /locus_tag="ACHKW0_003128"
     CDS             14755..16242
                     /gene="sufB"
                     /locus_tag="ACHKW0_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HFO7475918.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            16252..16998
                     /gene="sufC"
                     /locus_tag="ACHKW0_003129"
     CDS             16252..16998
                     /gene="sufC"
                     /locus_tag="ACHKW0_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HFO7475919.1"
                     /translation="MLSIKDLHVSVEGKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            16973..18244
                     /gene="sufD"
                     /locus_tag="ACHKW0_003130"
     CDS             16973..18244
                     /gene="sufD"
                     /locus_tag="ACHKW0_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HFO7475920.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPFLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            18241..19461
                     /gene="sufS"
                     /locus_tag="ACHKW0_003131"
     CDS             18241..19461
                     /gene="sufS"
                     /locus_tag="ACHKW0_003131"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HFO7475921.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            19474..19890
                     /gene="sufE"
                     /locus_tag="ACHKW0_003132"
     CDS             19474..19890
                     /gene="sufE"
                     /locus_tag="ACHKW0_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HFO7475922.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            20039..21043
                     /gene="ldtE"
                     /locus_tag="ACHKW0_003133"
     CDS             20039..21043
                     /gene="ldtE"
                     /locus_tag="ACHKW0_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HFO7475923.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(21107..21343)
                     /gene="lpp"
                     /locus_tag="ACHKW0_003134"
     CDS             complement(21107..21343)
                     /gene="lpp"
                     /locus_tag="ACHKW0_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HFO7475924.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         5608 a         5234 c         5436 g         5247 t
ORIGIN      
        1 tctgataaaa gatattcttt tctatctgaa taataattat cgcgaaaaaa tcactttaga
       61 acagttatcg aaaaaatttc gtgccagcgt cagttatatt tgccatgaat ttaccaaaga
      121 gtatcgtatt tcccctatta actatgttat tcaacggcgt atgacggaag cgaaatggtc
      181 actcactaat actgaattat cacaggcaga gatctcctgg cgtgtgggtt atgaaaatgt
      241 cgatcacttt gccaaactgt ttttgcgcca tgtcggttgt tcgcccagcg attaccgcag
      301 gcaatttaaa aactgttttg cggaacaaga aatcctatct gaatttcctc aaccggtaag
      361 tcttgccggg taaatctggc ggcgcagttt gttaagaagc tgcgccgcct gcagattatt
      421 tgttctgata atctttcagg atctgccgac ccgcaacgta aatcataatt tcgtctgtac
      481 cgccgccgat acgttcacaa cggacatcac gccagaagcg ggagacgcgc gcctcatcgg
      541 tatagcccag accgcccatg atttgaatcg catcatcaat gacttccatt gcggtacgtg
      601 cacagtacag ttttgccagc gccgcgctgg tgcgcagtga ctgatgctga tcggcttgcc
      661 atgccacttt cagcaccatg ttgcgcatgt tgtcgatctt aatcgccatc agcgccagtt
      721 tttcctggat catctggtta tgaccaatgg gcttaccaaa agcgatacgt tggttggcat
      781 agcgggcggc atcttcaaag gcgcattcgg caaagccggt gctgcgcgcg gcgttgatca
      841 ggcgctccat ctcaaagttg tacatcacat tgaggaaacc cattccttct tcgcccacca
      901 tgtcgctctc ttcaacctcc acgttgtcga gatagacttc gcaggtgctg agcatatgcc
      961 agccgatttt atgcagcggg ttaatcttaa tgccgggctt actggagtcg acccaccaca
     1021 gggtgaaggc ttttttggga tctttcggtt gcggatcgcg cgccagcacc agcatatacg
     1081 ggtactcttt cgcaccggta ataaaggttt tttgtccgtt gatgtagacc ttgccgtttt
     1141 tacgcgtgta agtggtagtg gcactgttgt tatctgagcc agcacctggt tccgtcaacg
     1201 ccagggcata ggcgggatca cccgtttcca gggtgctctc tgccgtttta cgtagctgct
     1261 ctgcagaacc gaaacggcgc atactgtgaa tacattgacc gttggtaatt aaaaatgccg
     1321 gagcaccgca ttttgacact tccatcagcg ccagcatttg ggtgacgtaa tccgcaggga
     1381 taccaccaaa ttcttccggc acgccaagca tggaaatacc gttatccgcc agcgcccgca
     1441 taaactcacg cggatatgtc ccgttttgat cgcaggtgcg gaaatactct tccggaaagt
     1501 tagtcgtaat cagttcgcga atactggcca gcagcagttc ttgttcttca gttaaagaaa
     1561 aatccatcat tatactcctg ttaatgagcc gcttcaggca ggacaaaacc catcgccgca
     1621 tcgataaata atctttcgtc catcagtttg agttctggcg aaatcactgg ggtgaagtcc
     1681 attttgtcga gaatatcttt ttgtaaatcg acgccagggg cgatttcgat taaatgcagg
     1741 ccgtcttctt tcagcgtgaa tacagcacgt tcggtgatgt aacgaacatc cagtccccgt
     1801 tcgagcgcga tttttccgct aaaagtaatt tccggtagtt cccgaataaa tttatttacc
     1861 cgtccttcct ggacgatatg caatttgcca tcggcaattt ctgtttttaa actgcccgca
     1921 gttaacgtgc cgcagaaaat aattttcttc gaggtggcac tgatatcaat aaatccaccg
     1981 gtacccatga ttttgccatt gaatttatgc acgccgacgt taccgtgctg gtcgacttca
     2041 gcaaaactca aataacaaac atccagacca ccaccgtgat aaaaatcaaa ctgggacgtc
     2101 atatccagaa tggcgcgggt attcacgttc gcgccaaagg cgataccctg tgaagtaatt
     2161 ccgccaatcg gacctgtttc taccgtcaga ataaagtcat cagcacaacc ttcttcccgg
     2221 gcgaccaggc caataccgtc agcaataccg acgccgacat tccccaccgc gcctttacgc
     2281 atttcgaata atgcacgccg cgcaactaat ttacgttggt ttaggggcag gctaagtttg
     2341 gtgctgtcat caagggtgaa gtcaccagaa ataaaacggt taaccggcgc accgccatac
     2401 agttggcttt gatccggatc gaccaccaca atatccacca gataacccgg aatacggaca
     2461 gatttaggat gcagcgtggc tttcttaacc attttctgca cctgcatcat cacaataccg
     2521 ccgttattgt gcaccgcctg ggcaataacc aatgcgtcga gatacatcac ctcatcttca
     2581 aaagtggcgt agccttcact gtcgcaggtg gtagcgcgaa tgaaggcgat atctggcgca
     2641 atcgctttgt aatagagata ttctttgtta tcaaactcga ccagtttaat caggtcttct
     2701 ttcgtgactt cattcagttt gccgccttgc tggcgtggat cgacaaatgt cccgatgcca
     2761 atatcactaa taataccagg ctggtgggct gcggcggcgc gtaaggtttg tgtaagtaca
     2821 ccttgtggat agttataagc aataatttta ttttgttctg cgagttcaga aatacgcggc
     2881 gattgtcccc agtgaccaca taatgcccat ttcaccagac cttcttgcgc cagaggacta
     2941 ataccacggt cggcgcgatc gccaagccct gtcggactaa taatcgataa attacgtggc
     3001 gtttgagtct gtttatattt atcagcaaga gctgtaatta acgtggtggc ttccagaata
     3061 ccgccgccag cgcctaacac acaaagtgtt gcttcgtcgg gaatataatt caccgcttcc
     3121 tgtgccgaca ggaccggcac tcgtccatta atacgaggtg gttttacagg tttcattgtt
     3181 aattcctgat tcagatgtga ttttccctac gccagatgga gaattagcgc acagagactc
     3241 acgaaatatt tttattaaat acggttaaat atatttttta agttcggcgg ggagggtgtt
     3301 cccgccgaaa tattattgct tatgcctggt gtaaaatagt taataccgtg cgtaaatcat
     3361 ttaccgagat ttgccctggt gcagaggctt tttttaccgc accaaaagtt gccgccgagc
     3421 caaatacttc accagccaga cgagaaatta cgccagtttt tgccatcgac atcgtgatga
     3481 ttggacgatc ggcatactgc tcctgcatct ccagggtcgc ggtaagcaac gtcagcacat
     3541 cgctggtact ttgcggcatc agcgcaatct taggaatatc ggcgtcgaag gactgcattt
     3601 tgcgcagacg ggcaatgatt tcttcggctt ccggcgtttt atggaagtca tggttggaca
     3661 tgacaacttt cacatcatgc gcgtgggcgt aggcgacggt ttctttaacc tgatcatcgc
     3721 cggtaaataa ctccagatcg atcatatcaa ccaggccgct gtcgatggct gcacgattga
     3781 gagcgatata agcctcggtg gaaatcgcct gctcgccgcc ttctttggca ctgcggaagg
     3841 taaacagcag cggtttttct ggcatggttt cacggagaat ttttgccgcc gccatgacag
     3901 actccacatt ggagaggtcg gcaaagtggt ccacacgcca ttccagaata tcaaagtccg
     3961 cttcacgata ggcgagagct tcggatttca cgcgggcgat atctttcgcc atcagcgaga
     4021 cgatgatttt aggtgcgccc gtaccaatga cgagatcttt tacagttacg gttttcattt
     4081 tttacccttt ctgcacgcgg tcagcctgtc aggcaccgaa ccccatgacc tgtttaacat
     4141 attccagagg gaaatctttg ccagtccata acgtgaactg ttcagcccct tgccacaaca
     4201 acatgccgta tccatcaatc gttttgcaac cagcttgttg cgcctgctgc aataacttcg
     4261 tcatatgcgg gttatacacg cattcagtga ccagcagtcc cggatgtaac agactgatat
     4321 cattaactaa tgattcattc tcaaggggtt tcatacccac ttttgtgcca ttggttaaaa
     4381 tgtcggcgga agccagggct tcagcaaagg cttgctgatc ggcgagatcg gtaaccgtga
     4441 cgacacaatc ggtgttttcg ttaacccgct gcgcgaaggc gagggcttta tcgaagaact
     4501 catcccgacg gttaaagagt ttaatttctt ttaaaccttc aattgccccc tgcgcgccaa
     4561 tcgccgttga ggcacctccg gcccctaaca gcaccattgt tttgcctttg atatcaaaac
     4621 cgctctcttt aatggcgcga atatggcccg tgccgtcggt gttatagccg cgcaggtagc
     4681 catcatcatt aacgatggtg ttgatggcac ccaccagttt ggctgctggc gttaattcat
     4741 caacatattc acacgccagt tgtttgttcg gcattgatat accagttccg cgcattttga
     4801 gggcttttaa tccttcaatt gctccgggaa aactatcgtt atccacttca aaggccatat
     4861 aggtaaatgg caatcccgct ttttctaagg ctttattctg catttcaggc gataaactgt
     4921 ggcggatagg ataggccatc agcccaatca attcgtattt tgcggtaaca tccataatta
     4981 actcctttaa cctctatgct taattgtgtt taaccgggtg ctaaatttac gctcgccaaa
     5041 ccgcacatca ttctcaggaa taataaaaac gcggtaatag cggataaaaa caataattgc
     5101 ggtaatcagg gccagcagcg cgaaagtaaa atcgagaaca atgatatatt gaaggccgat
     5161 gttcgacaga taaccggtaa tcagcggaat aacaaagtta gccagtccac ccatcatcat
     5221 ataaatactg gtgactttgg ctttgctttt tgggaaaaac tctgacatga ccgaaacacc
     5281 gagctgtaaa atgccgccag ctgctgagaa accgataaca aaggcgccgg cattacacac
     5341 cagtggagaa gggtacaggt agataatggc tgctgttatt gttgccagtg cagagttaaa
     5401 tacattagcc cagatgggcc ggaccatttt tttcagtagt gcggcaaaaa taaagacaca
     5461 gaccaacgag cccatactgt aataagagat ggtttttaat gcctcagctt ctgacatacc
     5521 agcaaaagcc atcgcatatt tgggcatcca caccacaatc acataaaagg tcgagaatgc
     5581 ggctacaccg aacagtaccg atgaaacacc ttccagccag actaacggtt tgctgttcat
     5641 ttgcggtaat tcattagcta cgctggcgtc caccaactgg ctggggaatt tgcttttcaa
     5701 cagcatcagc gtgatcagta caaacagaat acccggaata atcagcccat agccgtacca
     5761 gatattattg agcaacatat agctcaccag cattgggtag aacatttgcc caaatgacac
     5821 catcgcttta accagtatga ccgccgaacc agaggctttc ggaaagcatt ccatgagcgc
     5881 ggggtagcca cccgtatcca gcgctgagtt agcgatacct acgcacactg ccagaccgta
     5941 ggcgagagtt aaattcgggc aagcgggaat accaaagaag aatagcagat acattattac
     6001 tgccattaat atcaccgccc gacgaccaaa cttatcggag atcacaccga agaataaaat
     6061 actgatcaat cgccccaaac cgataccgga aattaagtag gcaatgcccg cgttgtcagt
     6121 ggaaaacttt tctgccagag atgacatatt ttgggcaagc gtaataacac taatgccgtg
     6181 caggaaatag ctgaagtaaa tacaaagaac agccaggata aatggcgtgc tgaaagcctt
     6241 attttgagac attttcacag ctccttgctg aaagataatt tcgttaccgt acgtattgtt
     6301 ctccgacggt ttacataatt actatactga agatgatttt ccctgggtac atggcataaa
     6361 gtttatgttt ccaggaatat tgcatgtcat agtttgtttt tgatcgcgat tttccttagt
     6421 tagcgcagaa agagttattt atccgtaaga tcttgcatga cctgaaaaga atattgcatt
     6481 acccacccgg agcgacattt tcctttagcg agtgatgccg cgtttttttg cggctgcgta
     6541 agccgataaa cagtgccagt aaaaaaccga tggcagcgat ggcggtatcg aaccacataa
     6601 tatcggcaat acttctttgg gacagatgag ccgtaatcaa cggaatagta aaggtcgcaa
     6661 tactgcccgc actgtaatag atccctgtag ctttaccttt agcgtaaggg aaacgttcag
     6721 ccattaacgt caggccaatt tgcacaacac ctccagcaga ggtaaaacca atgacaaaag
     6781 caaatattat caccacataa aatgtgggat gcaggcagac ggtaaacaga gcaataaatg
     6841 agaaaaaggt gtacagcatc agtaatgttg ttgggcgaac ggtattacga atgagtggag
     6901 cggtaataaa tacacaaagc agcgaaccca cggtgtagat actgagtagt ttaatcgaca
     6961 tagtgtatga catgcctgca acaaattgtc cgtactgtgc cagccactgg ctaaccagat
     7021 aaaacgttgc cattgagata tagccatata aggtataact ggctaaatcg ataattgaac
     7081 agcgatgttc cgtagagctg gtggttttct ttatgacagg taagcgacga cccggatggg
     7141 gtgggaacgt acaacggtat aaaaacagag cgttaataaa cataatgcct gcagcaatca
     7201 taaaggacca accgaaccac agttcagccc acactaacag gctaatgatt agcggtaata
     7261 aaaattgtcc gctggaaaca aatgctttaa ttaaaatatt ggctgtccca ggtgagcgtg
     7321 gaaaagcttc catcaaactg ggataagtgc ctgcatcgag aaaactgttt gccattcccg
     7381 ccagaaagcc aaaaacatag gcgataatga tgttattggt gtgcaggatg ccaaaaaaga
     7441 aggccatata gcagcacatc ccgagcatga taaaagggcg gcgaccaaag cgatcggata
     7501 ataatcctgc aaaaagcaag acacttaatc gaccaatgcc cagcgatgag ataactatcg
     7561 agacacccgc ggcattagtc tgccaaagtg tctccagcga ggccatattc aggctcatca
     7621 aaatgacgcc cataccatgc accaggtaat taaaatacaa cccaagtgcg gtagggtaat
     7681 agggattttt cataggttct acttagcaaa ggtgctttaa aaagtgtagc gatgttgtgt
     7741 cagtatccgt tcattattgt tgtaacaacg acaattcctt tttgtggaga aattaacaaa
     7801 gccgctccag atcgtgtgca tataattcag cggctaccga ttgatagatc ttccagtcaa
     7861 taaaattgcc atcagggtag acttgttgaa acgcggttaa gtcggggaaa aaacgcatgg
     7921 tgttgttgcc gatgcggtta tttattttct ctatgatggc atgaagatgt ttttttcgtt
     7981 gctgacgcat agccaacagt tgctcgacta cacgatcagg aatggcgcat ttgccatttt
     8041 cccactgacg ccagctttca ctgtcaccgg tctgggcaat ccaggtcgcg cattcatcaa
     8101 tagtcatggc aaaaatatgg cgaagtgcct ggagttcata agcgttcatc gtgtaatggc
     8161 tgcatgaaat aaacaataaa atataaataa cattatcagc ctgctgacgg cttgaagaga
     8221 gtcacagtat cttgtgcaac attattggaa agagtgaggg gcgaggtagc gaagcggaaa
     8281 aatgttaaaa aaaagcccat cgtgggagat gggcaaagac tacacacagc aattcgttgt
     8341 ttcactcagg ggatttccat gcttataaat caatatgttg atttataacc gtaagctaat
     8401 agtaggcata cggacttttt gcgtcgatca gattcgtctc attagtttaa aaactgtaaa
     8461 gaatttgcga caggtactac cagctgaatg gcggtaagca ggaaaatatg gggaagagta
     8521 agcaaatatc aattagcggg ggttaacaat caggcggcag agccgcctga tgagaaatta
     8581 cttattcggt ttctcgattt cgccaagttc ttcgagaatt tcttccggtt ggtccgtcgg
     8641 cggcgggact tcttccaccc acgcggcaaa cagacgccag gaaacggcta acagaaccgg
     8701 accaataaac agaccgatca tcccgaaagc aatcaaacca ccaataacac cagagaggat
     8761 caggatcagc ggtaaatcgg cacccatgcg aattaacatt gggcggatga cgttatccag
     8821 cgtgccaacc acaccgctcc acactaacaa taccgttccc caggtggtat cgccagtcca
     8881 gtagagccag ataatcgccg gaatcagtac cggcaacggg ccaagctgga caaggcagga
     8941 gaggatcatt aacaccgtta gcaacgttgc ataaggtacg ccggatacgg cgaggccgat
     9001 accgccaagc actgcctgta ctaacgccgt caccaccaca cccagcgcca ccgcgcggat
     9061 agcctgtgcc gccagcagta cggcggcatc tccgcgaacg cctgccagac gggttgcaaa
     9121 atggcgaatg ccttgtgcca cctgttcacc gcgccagtac agcagggcac tgaagagaag
     9181 catcagcgca caatgcacca taaagcgccc gatatgcgcc gcctgcccaa cgaaccaggt
     9241 ggtggtggtg ccaatataag ggcggacttt cgccatgatc gccgtccccc ccatatccag
     9301 caagttgtgc cagcctgcat acagcttcgc gccaatcacc ggaatggtat taagccacgc
     9361 cagatcgggt aacgtcatgt cgccgctgga aatggcttta attagcgggc cgctgccgtc
     9421 gacgatactg ttaaccagca aagcaatagg gatgataaac accatcacta ataacagcgt
     9481 catcaccaga acggcgagag agcggcggcc aaacatgatc ttttgcaaac gtaacaatac
     9541 cggccaggtg gcgataacca ccgtaccggc ccatgcaaag ccgagaataa agggttgaac
     9601 aatccacaga catgccacaa tcatgatggc taaaaacagc accgaaagca gaatttgtgc
     9661 gacatccctg ggctgacgaa catttaccat aactactttt cacctttttt gtgcgtcaaa
     9721 tcgtcgacgc gacgtgaaca cgcgaaactc acctgcataa tgatgagcaa tttcagcggt
     9781 ttttaacagg ccgattctgc ccttaattct gatgggcgtg ttagaaaaaa atgtggtaca
     9841 acaactcagg caacacgcaa acgatttact cgtcgtattt caagttgcat gtgctgcggc
     9901 tacgttcgtt caccccagtc acttactatt gtaagctcct ggagttcact cgcttgccgc
     9961 cttcctgcaa ctcgaaatcc atagagtaaa taaacatcac ctttcagtgc gctattgcag
    10021 ccgcctgaaa gaatagtgta tcgacctaca tcacagaccg caggaaaggg tcaatataat
    10081 gattccacag atttcccagg cacccggcgt cgttcaactg gtgcttaatt ttttgcaaga
    10141 gctggagcaa caaggtttta ccggcgatac ggcgacaagt tatgccgatc gtctgacaat
    10201 gtcgaccgac aacagtattt accaacttct ccccgatgcg gtggtatttc cgcgttcaac
    10261 cgcagatgtg gcgctgatcg cccgtcttgc cgcgcaggaa cgctattcat cgctgatctt
    10321 taccccccgt ggcggcggca ccggcactaa cggtcaggcg ctcaaccagg ggattattgt
    10381 tgatatgtcc cgccatatga accgcatcat cgaaattaac cctgaagagg gctgggtgcg
    10441 cgttgaggcc ggggtgataa aagatcaact caatcagtac ctgaaaccgt tcggctactt
    10501 ttttgcgccg gaactttcga ccagcaaccg ggcaacgctc ggtgggatga tcaataccga
    10561 tgcttctggt cagggatcgc tggtctatgg caaaacgtcg gatcacgtac ttggcgtacg
    10621 tgcggtgttg ttggggggcg atattctcga tacgcaacct ttacccgtcg aattggcgga
    10681 aacgctgggt aaatccaata ccacaatcgg gcgaatttat aacacggttt atcaacgttg
    10741 ccgtcagcaa cgccagttaa tcatcgacaa cttccccaaa cttaaccgct ttcttaccgg
    10801 ttacgatctg cgtcatgtct ttaacgatga gatgaccgag ttcgacctga cgcgcattct
    10861 gacgggttca gaagggacgc tggcctttat taccgaagcg cggctggata ttacgcgctt
    10921 gcctaaagtg cgccgtctgg tgaatgtcaa atatgactct tttgactctg cgctgcgtaa
    10981 cgcgccgttt atggttgagg cgcgggcgct ttcggtagag acggtggact caaaagtgct
    11041 gaatctggcg cgggaagata ttgtctggca ttccgtcagc gagttgatta ccgatgtgcc
    11101 tgacaaagag atgctcggac tgaacattgt ggaatttgct ggtgatgatg aggcgctgat
    11161 tgacgagcga gttaatgcac tctgtgtgcg gcttgatgag ctgattgtca gccaacaggc
    11221 aggtgtgatt ggctggcagg tgtgccgcga gctggcgggt gttgaacgta tctatgcgat
    11281 gcgcaaaaaa gccgttggtc tgcttggcaa tgccaaaggt gccgctaagc caattccgtt
    11341 tgctgaggat acctgtgtac cgccggaaca tctggcggat tatattgctg aatttcgcgc
    11401 gctgctcgac agccacggct taagctacgg tatgttcggt cacgtcgacg caggtgtttt
    11461 gcacgtccgt ccagcgctgg atatgtgcga tcctcaacaa gagattttga tgaagcaaat
    11521 ctctgatgac gtggtggcgc tgactgcgaa atacggtggt ttgttgtggg gcgagcacgg
    11581 caaaggtttt cgcgctgaat acagcccggc gtttttcggt gaggaacttt ttgcagaact
    11641 gcgcaaagtg aaagcggcat ttgacccgca taaccgactc aacccaggga agatttgccc
    11701 gccagaaggt ctcgatgcgc cgatgatgaa agtggacgcg gtgaagcgcg gtacattcga
    11761 tcggcagatc tccattgcgg tacgccagca gtggcgcggt gcgatggagt gtaacggcaa
    11821 cggtttatgc ttcaactttg atgcccgtag tccgatgtgt ccgtcgatga agatcaccca
    11881 gaaccggatt cattcaccga aagggcgcgc aacgctggtg cgtgaatggc tgcgtttgtt
    11941 ggcggatcgc ggcgttgatc cactcaaact ggaacaagaa ctgcctgaat ccggcgtcag
    12001 tttgcggacg ttaattgccc gcacgcgcaa tagctggcat gcgaataaag gcgaatatga
    12061 cttctcacac gaagtcaaag aggcgatgtc gggctgtctg gcctgtaaag cgtgttcgac
    12121 ccagtgcccc atcaaaattg atgtgccgga gtttcgctct cgttttctgc agctctatca
    12181 cacccgttat ttacgcccgc tgcgcgacca cctcgtcgct acggtcgaga gctacgcgcc
    12241 gctgatggca cgcgcgccga aaacctttaa cttcttcatt aaccagccgc tggtgcgcaa
    12301 actctcggaa aaacatatcg gcatggttga tttgccgctg ttgtcggtcc cctcgctaca
    12361 acaacaaatg gtggggcatc gctcggcaaa catgacgctg gaacagcttg aatctctcaa
    12421 tgcagagcag aaagcgcgca cagtgttggt ggtgcaggac ccctttacca gctattacga
    12481 tgcgcaagtg gtggcggatt ttgtccgtct ggtcgaaaaa ttaggtttcc agcctgtgtt
    12541 actgccattt tcgccaaatg gcaaagccca gcatattaaa ggttttctta atcgttttgc
    12601 gaagacggcg aaaaagacgg cggatttcct caaccgtatg gcgaagctgg gtatgccaat
    12661 ggtgggcgtc gatccggcgc tggtactttg ttatcgcgat gaatataaac tggcgctggg
    12721 cgaggaacgt ggcgagttta acgtcttact ggcgaatgaa tggctggcaa gcgcacttga
    12781 gtcacagcca gtggctacag tcagcggtga atcatggtat ttctttggtc actgtaccga
    12841 agttaccgcc ttgccgggtg cgccagcaca atgggccgcg atatttgccc gttttggcgc
    12901 gaaactggaa aatgtcagcg tgggttgctg cggcatggca gggacttacg gacatgaagc
    12961 gaaaaaccat aaaaattcgc tcgggatcta tgagttatcc tggcatcagg ctatgcagcg
    13021 actgccgcgt aaccgctgtc tggcgaccgg atattcctgc cgtagccagg taaaacgggt
    13081 tgaaggcacg ggggtacgcc atcctgtgca ggctttactg gagattatta aatgatatgg
    13141 aaacggaaaa tcaccctgga agcactgaat gctatgggtg aaggaaacat ggtggggttc
    13201 ctggatattc gctttgaaca tattggtgat gacacccttg aagcgacaat gccagtagac
    13261 tcgcggacaa agcagccttt cgggttgctg catggaggag catccgtggt actggccgaa
    13321 agtatcggtt ccgttgccgg ttatttatgt accgaaggtg agcaaaaagt ggttggtctg
    13381 gaaatcaatg ctaaccacgt ccgctcggca cgagaagggc gggtgcgcgg ggtatgcaaa
    13441 ccgttgcatc tcggttcgcg tcaccaggtc tggcagattg aaatcttcga tgagaaaggg
    13501 cgtttgtgct gttcgtcacg attgacgacc gccattttgt gacgttgtca ccagaaaagt
    13561 gtgacgcata ttgcctttgg tcattatctg cacagtaaac ggtggtatac tgtgcaggtc
    13621 ttgtctaaaa gcgaggatgc tatgtctact caacttgatc ccacccagct ggccattgaa
    13681 tttttacgcc gtgaccagag caatctttca cctgcccaat atctcaaacg tctgaaacaa
    13741 ctagaacttg aatttgctga cctgcttact ctctcttctg ctgaattaaa agaagaaatc
    13801 tactttgcct ggcgtctggg cgtgcattga tcctgactta atgccgcaag ttcgccaaat
    13861 cgacctggat taacctccgt ccaggttcga tgaccttctt aatactgctt atctaccgcc
    13921 tgatattgtt caaaaataat actaatcgca gtttgtgtta aaacggcggg ttagctttat
    13981 gagattattc ttatcgcccc ttcaagagct aagccactga gagtgccgga gataagcgcc
    14041 ggatggggta gaaaccctta agcctgtgtt gcacagactt aagggttttc ttatttctga
    14101 acgctgcact tttttaccta tctcttaaat agtcattttc aatacaactt tttctttttc
    14161 atcacagcct taatgctcct tagataataa ccattatcta acaatgagat acctaattct
    14221 tagcgtgcct gttaacccac acatcagggt ctatgcttat taaaagcagg caaaaagcat
    14281 gttttacggt aaagcccctg cgtttgcttg gttgaactga taatcattat cactaacatg
    14341 ctgttatacg ctgaaagcga tgaagtgagg taaatcgatg gatatgcatt caggaacctt
    14401 taacccacaa gatttcgcct ggcaaggctt aacgctgaca cccgcagcgg cgatacacat
    14461 ccgtgagctg gtggcaaagc agccgggtat ggtcggcgtg cgcttaggcg tgaagcaaac
    14521 gggctgcgcg ggctttggct atgtgctcga cagtgttagc gagccggaca aagacgatct
    14581 gctgtttgaa cacgacggcg cgaagctgtt tgtcccgctg caagcgatgc cgtttattga
    14641 tggcacggaa gtcgatttcg ttcgtgaagg acttaatcag atattcaaat ttcacaaccc
    14701 taaagcccag aatgaatgtg gctgtggcga aagctttggg gtataggcgg tactatgtct
    14761 cgtaatactg aagcaactga cgatgtcaaa acctggaccg gcggcccgct gaattataaa
    14821 gaaggattct tcacccagtt agccaccgat gagctggcaa aggggataaa cgaagaggtg
    14881 gtgcgcgcaa tttcggcgaa gcgtaatgag ccggagtgga tgctggagtt tcgtctaaac
    14941 gcctatcgcg catggctgga gatggaagaa ccgcactggt tgaaagcgca ctacgacaag
    15001 ctgaattatc aggattacag ctactactca gcaccatcgt gcggtaattg tgacgacact
    15061 tgcgcgtctg aacctggcgc ggtgcagcaa actggcgcga acgccttttt aagtaaagag
    15121 gtggaggcgg cgtttgagca gttgggcgtt cccgtgcggg aaggcaaaga ggtggcggtg
    15181 gatgccattt tcgactcagt ttcggttgcc actacttatc gcgaaaaact ggcggagcag
    15241 ggaattattt tctgttcctt tggtgaggcg atccacgatc acccggaact ggtgcgtaaa
    15301 tatctcggca ccgtggtgcc ggggaatgac aacttctttg ccgcgcttaa tgcggcggta
    15361 gcctctgacg gtacgtttat ttatgtgcct aaaggcgtgc gctgcccgat ggaactttcc
    15421 acctattttc gcattaacgc ggaaaaaacc gggcagtttg agcgtaccat tctggtggcc
    15481 gacgaagaca gctacgtcag ctacattgaa ggctgttccg ctccggtgcg tgacagctat
    15541 cagttacacg cggcagtggt ggaagtcatc atccataaaa acgccgaggt gaaatattcc
    15601 acggtacaaa actggttccc tggcgataac aacaccggcg gtattctcaa cttcgtcacc
    15661 aagcgtgctt tgtgcgaagg cgaaaacagc aaaatgtcat ggacgcaatc agaaaccggg
    15721 tccgcgatta cgtggaaata tcccagctgc attttgcgcg gcgataactc cattggtgag
    15781 ttttactcag tggcgctgac cagcggtcat cagcaagcgg ataccggcac caagatgatc
    15841 cacatcggta aaaacaccaa atcgaccatt atctcgaaag ggatctctgc cggacatagt
    15901 cagaacagtt atcgcggctt agtgaaaatc atgccgacgg caaccaatgc gcgcaatttc
    15961 actcagtgcg actcaatgct gattggcgct aattgtgggg cgcatacctt cccgtatgtc
    16021 gaatgtcgca ataacagcgc acaactggag cacgaggcaa cgacatcacg tattggtgaa
    16081 gatcaactgt tttactgcct gcaacgcggg atcagcgaag aagacgccat ctcgatgatt
    16141 gttaacggtt tctgcaaaga cgtgttctca gaactgccgc tggaatttgc cgttgaagca
    16201 caaaaactcc tcgccatcag tcttgaacac agcgtcggat aaggaataaa catgttaagt
    16261 attaaagatt tacacgtcag cgtggaaggt aaagctatcc tgcgtggatt aagcctcgcc
    16321 gttcgtcccg gcgaagttca cgccattatg gggccaaacg gttcgggcaa aagtacctta
    16381 tcggcaacgc ttgccgggcg cgaagattat gaagtgacgg gcggcacggt tgagttcaaa
    16441 ggcaaagatt tgcttgcact gtcgccggaa gatcgtgcag gcgaaggcat ctttatggcc
    16501 ttccagtatc cggtggagat tccaggcgtc agtaaccagt ttttcctgca aacggcactt
    16561 aatgcagtgc gcagctatcg cggccaggaa acgctcgacc gctttgattt tcaggatttg
    16621 atggaagaga aaatcgctct cctgaagatg ccggaagatt tattaacccg ttcggtaaac
    16681 gttggttttt ccggcggcga gaaaaagcgc aacgatattt tgcaaatggc ggtgctggaa
    16741 ccggagttat gcattcttga tgagtcggac tccgggctgg atattgacgc attaaaagtg
    16801 gtcgccgatg gcgtgaactc gctgcgtgat ggcaagcgct cattcatcat tgttacgcac
    16861 taccaacgca ttctcgacta catcaagcct gattacgtcc atgtgctata tcagggacga
    16921 attgtgaaat ccggcgattt cacgttggtc aaacaactgg aggagcaggg ttatggctgg
    16981 cttaccgaac agcagtaacg cgctgcaaca gtggcatcac ttgtttgaag ctgaagggac
    17041 aaaacgctca ccgcaagcac agcagcattt acaacaattg ctgcgtaccg gactgccgac
    17101 acgtaaacat gaaaactgga aatatacgcc gctggaaggg ctgaccaata gccagtttgt
    17161 cagcattgcg ggagagatat ccccacagca gcgagatgcc ttagcgttaa cgttagacgc
    17221 tgtgcggctg gtatttgtcg atggacgtta cgtgtcagca ctgagcgatg cgactgaagg
    17281 cagcgggtat gaagtgagca ttaacgacga ccgtcagggg gtacccgacg ctattcaggc
    17341 ggaagtgttt ctgcatttga cggaaagcct ggcacaaagc gtgacgcata tcgccgtgaa
    17401 gcgcggtcaa cggccggcaa agccatttct gttaatgcat attacccagg gcgtggcagg
    17461 tgaagaggtc aacactgccc attaccgaca tcatctggat ctggcggaag gtgccgaagc
    17521 aacggtgatc gaacattttg tcagccttaa tgatgctcgt cacttcaccg gcgcacggtt
    17581 cactatcaat gtcgcagcga acgcccactt gcagcatatc aagctggcgt ttgaaaaccc
    17641 ggtcagtcac cactttgccc ataacgattt gttgctggct gacgatgcca ccgcatttag
    17701 ccatagtttc ctgctgggtg gcgcagtgtt acgacacaac accagtacgc aactcaatgg
    17761 cgaaaacagc acgctgcgga tcaatagcct ggcgatgccg gtgaaaaacg aggtgtgtga
    17821 tacccgtacc tggctggaac acaataaagg tttttgtaac agccgacagt tgcacaaaac
    17881 tatcgtcagc gacaaaggcc gcgcggtatt taacggtttg atcaacgtcg cgcagcacgc
    17941 catcaaaacg gatggtcaga tgaccaacaa taatctgctg atgggcaaac tggcagaagt
    18001 ggatacgaaa ccgcagctgg aaatctatgc agatgatgtg aaatgcagcc acggcgcgac
    18061 ggtggggcgt attgatgatg aacagatgtt ctatctgcgc tcgcgcggga tcaatcagca
    18121 ggatgcccag cagatgatca tttacgcctt tgctgccgaa ctgacggaag cactgcgtga
    18181 tgaggggctt aaacagcagg tgctggcccg aatcggtcaa cgactgccag gaggtgcaag
    18241 atgacttttt ccgtcgacaa agtgcgggcc gactttccgg tgctttcgcg tgaggtaaac
    18301 ggtttgccgc tggcttatct cgacagcgcc gccagtgcgc agaaaccaag ccaggtgatt
    18361 gactccgaag ccgagtttta tcgtcacggc tacgcggcgg tgcatcgcgg tattcatacc
    18421 ttaagcgccc aggcgaccga gaaaatggag aacgtgcgca agcgggcatc gctgtttatt
    18481 aatgcccgtt cggcggaaga gctggtgttc gtccgcggca cgacggaagg gatcaatctg
    18541 gtcgccaata gttggggcaa cagcaacgtg cgggcgggcg ataacatcat catcagtcag
    18601 atggagcacc acgctaacat tgttccctgg cagatgcttt gcgcacgcgt tggcgcagag
    18661 ctgcgtgtga tcccgctcaa tcccgacggt acgctgcaac tggagacgct acctaatctg
    18721 ttcgatgaga aaactcgcct gctggcaatt actcatgtct ccaacgtgct tggcacagaa
    18781 aatcccctgg cggaaatgat cacgcttgcg catcagcatg gcgcaaaagt gctggtggat
    18841 ggcgctcagg cggtgatgca tcatccggtg gacgttcagg cgctggattg cgatttttac
    18901 gtattctccg ggcataaact gtatggcccc accggaattg gcattcttta tgtgaaagaa
    18961 gccttgttgc aggagatgcc gccgtgggaa gggggcggtt ctatgatcgc caccgtcagc
    19021 ctgagtgaag gcactacctg gaccaaagca ccatggcggt ttgaagccgg tacacccaat
    19081 accgggggca tcattggtct tggcgcggcg ctggagtatg tttcggcgct ggggcttaat
    19141 agcatagccg agtatgaaca gaatctgatg cactacgcgc tatcacagct ggaatctgta
    19201 ccggatctca ctctctatgg cccgcggaac aggcttggcg ttattgcttt taatcttggt
    19261 aaacaccacg cctatgatgt tggcagtttt ctcgataact acggcattgc tgtgcgtacc
    19321 ggacatcact gcgctatgcc attaatggcc tattacaacg tccctgctat gtgtcgggcg
    19381 tcgctggcca tgtataacac ccatgaagaa gtggatcgtc tggtgaccgg actgcaacgt
    19441 attcaccgtc tgctgggata acagggaggc actatggctt tattgccgga taaagaaaaa
    19501 ttgctgcgta attttttacg ctgcgccaac tgggaagaga aatatctcta cattattgag
    19561 ctgggccagc gtctgccaga attacgcgcc gaagaccgaa gcccacaaaa tagcattcag
    19621 ggctgccaga gtcaggtgtg gattgtcatg cgccagaatg cgcagggaat tattgaattg
    19681 cagggcgaca gcgatgcggc gattgtgaaa gggcttattg cggtcgtctt tattctctac
    19741 gatcagatga cgccgcagga tattgtcaat ttcgatgtgc gtccgtggtt tgaaaaaatg
    19801 gcgctcaccc aacatctcac cccatctcgt tcacaaggtc tggaagcgat gattcgcgca
    19861 attcgcgcca aagccgctgc acttagctaa actaaaggga cagctttcat ccggttggct
    19921 cacattgccg gatggtcaat gtttacactg acctgcacag gagtgttttg gtttcaggtg
    19981 aacataagcg ccatccgacg tttcagcgtg agtctccggc aaatacagga ggtttacaat
    20041 gaaacgcgcg tctttgctta cactcacgct tatcggcgct tttagcgcca tccaggctgc
    20101 ctgggcggtt gattatccgc taccaccaac cggaagccga ctggttgggc aaaatcaaac
    20161 gtatacggtg caagaagggg ataaaaacct tcaggctatt gcccgacgtt ttgatactgc
    20221 ggcaatgttg atccttgaag ccaataacac tatcgccccg gtgccaaaac ctggtacgac
    20281 gataactatt ccttcacaac tgttattacc tgatgcaccg cgtcagggga ttatcgttaa
    20341 ccttgcagag ctgcgccttt attattatcc gccgggagaa aatattgtgc aggtttatcc
    20401 aataggtatt ggattgcagg ggctggaaac gccggtgatg gaaacgcgtg ttgggcagaa
    20461 aatccctaac ccaacctgga cgcctacggc aggcattcgt cagcgttcgc tggagcgtgg
    20521 cattaaatta ccgccagtcg ttcctgccgg accaaataac ccgctaggac gttacgcact
    20581 gcgcctcgcg catggtaatg gcgaatacct cattcatggc accagtgcgc cggacagcgt
    20641 tggtttgcgc gtcagttcag ggtgtattcg catgaatgca ccggatatta aagccttgtt
    20701 ctccagcgtg cggacgggaa cgccggtgaa agtgatcaac gaaccggtga aatattccgt
    20761 ggaacctaac gggatgcgtt atgttgaagt acatcgacca ctatcagcag aagaacagca
    20821 aaacgttcag acaatgccat acacactgcc agcaggcttt acgcaattta aagacaataa
    20881 ggctgtagat cagaagttag tcgataaagc gttgtatcgt cgggcagggt atccggtttc
    20941 ggtgagcagt ggagcaactc ccgcagccag caatgcgcct tcagtagagt cagcgcagaa
    21001 tggtgaacca gagcaaggga atatgttacg cgcgacgcag tagcggtaaa cggcagacaa
    21061 aaaaaatggc gcacaatgtg cgccattttt cacttcacag gtactattac ttgcggtatt
    21121 tagtagccat gttgtccaga cgctggttag cacgagctgc gtcatcttta gcagcctgaa
    21181 cgtcggaacg cattgcgttc acgtcgttgc tcagctggtc aactttagcg ttcagagtct
    21241 gaacgtcaga agacagctga tcgattttag cgttgctgga gcaacctgcc agcagagtag
    21301 aacccaggat taccgcgccc agtaccagtt tagtagcttt cattattaat accctctaga
    21361 ttgagttaat ctccatgtag cgttacaagt attacacaaa gttttttatg ttgagaatat
    21421 ttttttgatg ggaaggcact tatttttgat cgttcgctca aagaagcatc gaacgctggg
    21481 ttttagataa aaaaattgag agaaaacagg atgcttccat cggat
//
LOCUS       DAZFXT010000078        21063 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.078, whole genome shotgun sequence.
ACCESSION   DAZFXT010000078 DAZFXT010000000
VERSION     DAZFXT010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 21063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.078"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            218..718
                     /gene="tssB"
                     /locus_tag="ACHKW0_003135"
     CDS             218..718
                     /gene="tssB"
                     /locus_tag="ACHKW0_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7475925.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            753..977
                     /locus_tag="ACHKW0_003136"
     CDS             753..977
                     /locus_tag="ACHKW0_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475926.1"
                     /translation="MTAHKNISETYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1028..2503
                     /gene="tssC"
                     /locus_tag="ACHKW0_003137"
     CDS             1028..2503
                     /gene="tssC"
                     /locus_tag="ACHKW0_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7475927.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPWSPADNPVKSFNYHEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAR
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2510..2923
                     /gene="tssE"
                     /locus_tag="ACHKW0_003138"
     CDS             2510..2923
                     /gene="tssE"
                     /locus_tag="ACHKW0_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HFO7475928.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2927..4777
                     /gene="tssF"
                     /locus_tag="ACHKW0_003139"
     CDS             2927..4777
                     /gene="tssF"
                     /locus_tag="ACHKW0_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HFO7475929.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQMEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWSLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4741..5823
                     /gene="tssG"
                     /locus_tag="ACHKW0_003140"
     CDS             4741..5823
                     /gene="tssG"
                     /locus_tag="ACHKW0_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HFO7475930.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLDHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPELSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5848..7128
                     /gene="tagH"
                     /locus_tag="ACHKW0_003141"
     CDS             5848..7128
                     /gene="tagH"
                     /locus_tag="ACHKW0_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HFO7475931.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGTPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHLAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHKW0_003142"
     CDS             7125..7649
                     /gene="tssJ"
                     /locus_tag="ACHKW0_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HFO7475932.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGTGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7652..8983
                     /gene="tssK"
                     /locus_tag="ACHKW0_003143"
     CDS             7652..8983
                     /gene="tssK"
                     /locus_tag="ACHKW0_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7475933.1"
                     /translation="MATTRNKVMWQEGMLMRPHHFQQQQRYNDYLDNQRFRAMNDLSW
                     GFTELTLNNELLAQGKIMIDSASGTLPDGTVFSIPDQDALPDPLHPQNFPDERSRNIY
                     LALPVASDVRNEISDGRRIGRYRLNYADVRDLHSEEGDARTLTLGQLTPRIMSGAEDM
                     SAYITLPLCRISDRHADGSLTLDDDFIPSCQNIQVSKKLRVYLKEVQGAIGGRASDLA
                     NRIGSPAQSGIADVAEFMMLQLLNRNQTRFTHRARRSQLHPEDFYLDLAGLLGELMTF
                     TEPSRLPCPLDVYDHHDLTKTFKTLLPEVKRALHTVLSPRAVNLPLHLRDGIWQADIH
                     DTELLQSATFVLAVAANVPVDQIQRQFIQQSKISSPEKIRNMVSVQIPGIPLRAIMVA
                     PRQLPYHSGFSYFELDKSGQAWTEMAAAGAVALHVSGSFPDLNMQLWAIRG"
     gene            8988..9749
                     /gene="icmH"
                     /locus_tag="ACHKW0_003144"
     CDS             8988..9749
                     /gene="icmH"
                     /locus_tag="ACHKW0_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HFO7475934.1"
                     /translation="MDEGSLSLPPFTGYDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYIPCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9758..12513
                     /gene="tssH"
                     /locus_tag="ACHKW0_003145"
                     /gene_synonym="clpV"
                     /pseudo
     CDS             9758..12513
                     /gene="tssH"
                     /locus_tag="ACHKW0_003145"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12510..13253
                     /gene="vasI"
                     /locus_tag="ACHKW0_003146"
     CDS             12510..13253
                     /gene="vasI"
                     /locus_tag="ACHKW0_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     VasI"
                     /protein_id="HFO7475935.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKHRQGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13258..14670
                     /gene="tssA"
                     /locus_tag="ACHKW0_003147"
     CDS             13258..14670
                     /gene="tssA"
                     /locus_tag="ACHKW0_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7475936.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDGRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIR
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14779..18213
                     /gene="tssM"
                     /locus_tag="ACHKW0_003148"
     CDS             14779..18213
                     /gene="tssM"
                     /locus_tag="ACHKW0_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HFO7475937.1"
                     /translation="MLILAWIFLLVWIWWKGPTWTLYEEQWLKPLANRWLATAAWGII
                     ALMWLTVRVMKRLQQLEKQQKQQREEAIDPLSVELNAQQRYLDRWLLRLQRHLDNRRF
                     LWQLPWYMVIGPAGSGKTTLLREGFPSDIIYAPEGSRGAEQRLYLTPHVGKQAVIFDI
                     DGTLCAPADADILHRRLWEHTLGWLKEKRARQPLNGIILTLDLPDLLTADKRRREYLL
                     QTLRSRLQDIRQHLHCQLPVYVVLTRLDLLQGFAALFQSLNRQDRDAILGVTFTRRAH
                     ENDDWRTELNAFWQTWVDRMNLALPDLMVAQTHTRASLFSFSRQMQGSREPLVSLLEG
                     LLDGENMNVMLRGVYLTSSLQRGQMDDIFTQSAARQYRLGNNPLASWPLVDTAPYFTR
                     SLFPQALLAEPNLATESRDWLMRSRRRLTVFSATGGVAALLLITGWHHYYNGNYQSGI
                     TVLKQAKAFMDVPPPQGKDDYGNLQLPLLNPVRDATLAYGDWGDRSRLADMGLYQGRR
                     IGPYVEQTYLQLLEQRYLPSLFNGLVKAMNAAPPESEEKLAVLRVMRMLEDKSGRNNE
                     VVKQYMAKRWSEKFHGQRDIQAQLMSHLDYALAHTDWHAERQAGDGDAISRWMPYDKP
                     VVSAQKELSKLPVYQRVYQSLKTRALGVLPADLNLRDQVGPTFDQVFTSADDNKLVVP
                     QFLTRYGLQSYFVKQRDELVELTAMDSWVLNLTRSVKYSDADRAEIQRQLTEQYISDY
                     TATWRAGMDNLNIRNFESIGQLTGALEQVISGDQPLQRALTVLRDNTQPGVFSEKLSA
                     KERDEALAEPDYQLLTRLGHEFAPENSTLAVQKDKESTMQAVYQQLTELHRYLLAIQN
                     APVPGKSALKAVQLRLDQNSSDPIFATRQMAKTLPAPLNRWVGRLADQAWHVVMVEAV
                     HYMEVDWRDSVVKPFNEQLANNYPFNPRSAQDASLDAFERFFKPDGILDTFYQQNLKL
                     FIDNDLSLEDGDNSVIIREDIIAQLKTAQKIRDIFFSKQNGLGTSFAVETVSLSGNKR
                     RSVLNLDGQLVDYSQGRNYTAHLVWPNNMREGNESKLTLIGTSGNAPRSISFSGPWAQ
                     FRLFGAGQLTGVQDGNFTVRFSVDAGAMTYRVHTDTEDNPFSGGLFSQFGLSDTLY"
     gene            18224..19673
                     /locus_tag="ACHKW0_003149"
                     /pseudo
     CDS             18224..19673
                     /locus_tag="ACHKW0_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VasL domain-containing protein"
     gene            19600..20082
                     /locus_tag="ACHKW0_003150"
     CDS             19600..20082
                     /locus_tag="ACHKW0_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7475938.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            20126..21040
                     /locus_tag="ACHKW0_003151"
     CDS             20126..21040
                     /locus_tag="ACHKW0_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7475939.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT         5037 a         5685 c         5771 g         4570 t
ORIGIN      
        1 gcacaggttc acacaatcta tttaacgctt gcagaatacc cctctttacg atcgataaaa
       61 agtccttttc ttatttattt tgaagcgtaa aattcggggt gatgaaggag tggtgatttt
      121 attttcaccg ccattagggc ggcattgccg atattttttt gcggtacaaa tatgccgtgt
      181 tcatggcctg tacgagataa catgacggat cacttttatg agcaaaaaat ttgaaggcag
      241 cgtcgcgcct cgcgagcgta ttaatatatc gtatgtccca aaaacagatg gccagacggc
      301 tgaagtggaa cttccgctta acatgctcgt tgtcggcgat accggaaata cgcaggaaac
      361 atccccactg gatgaacgtc aggcagtttc cgttaacaag cacaatttcg gtgcagtgat
      421 ggctgaagca gccattggcc tcaattttac tgtccccgcc acgctgaagg gaagcacaac
      481 agacgatgaa ctgaatgtgg cgctgaatat caaatcactg gatgattttt caccagatag
      541 cgtggcccgg caggttccgg aagtgaataa attgctggaa ctgcgtgaag cactgactgc
      601 actgaagggg ccgatgggta accttccggc attccgtacc cagctccagg cattgctgga
      661 aaatgaagag tcccgcgagc agttgctcaa agaaatcggt ctggtgagca ataaataacc
      721 accactattt cattaaacag ggttatccag gcatgacggc acacaaaaat atctccgaga
      781 cataccacct ctctgttgca gacattctgc aggtggttta ccaggtatgt ttcagcccgt
      841 ccgttgaaat caaccaggat ggtgtggctg cgctgattac aacactggat cgccgtatca
      901 gtgatctatt ggatgagatt atccattttt gtgagtttca gcagagcgcg tcgcactggc
      961 agcgcgtact tcactgatgg cgaaaagccg gtttcagaaa cacagctaaa acagaaggat
     1021 tttcagtatg tctttacagg aagaggaact cgtttccagt catgccgggc agcctgagca
     1081 ggcatcgtcg ctgcttgatc aaatcatggc acagacccgt attcagcccg gttcggaagg
     1141 ttatgacgtt gcccgccagg gtgtgacagc ctttattgcc agcatcctgc aatccactgc
     1201 atctgccgaa ccggtcaata aactggccgt ggacagcatg attgcagata ttgatgaacg
     1261 catcagccgc cagatggatg tcatcatcca tgcgcctgcc tttcagcagg tcgaatcgtt
     1321 ctggcgctca ctgaaaacca tggtggatcg cgttgatttc cgtgaaaaca tcaaggtcaa
     1381 cgtgcttcat gtcaccaagc aggaacttct ggaagacttt gagtttgcgc cagaaattat
     1441 tcagtccgga ttttataagc acgtttattc gtccggattc ggacagtttg gtggtgaacc
     1501 catcgctgcc gtgctgggcg cttatgagtt caaaaatacc gcacctgaca tgaaactgct
     1561 gcaatacgtc agtgccgtgg gcgcaatggc gcacgcaccg ttcctgtcct ccgtttcccc
     1621 ggagtttatg gggctgaact cgtggaccga actgcctaat atcaaagatc tttatgccat
     1681 ctttgaaggt ccggcctaca ccaaatggcg cgccctgcgt gactcggaag attcgcgcta
     1741 tttagggctt actgcacccc gcttcctgct tcgccagccc tggtcaccag cagataaccc
     1801 tgttaaaagc tttaactatc acgaagatgt cagccagaac cacgaagatt atctgtgggg
     1861 taatacggcc tggatgctgg catgcaatat cgcagacagt tttgccaaat accgctggtg
     1921 tccaaacatt atcggcccgc aaagcggcgg cgcggtgaaa gatctgccgg tgcatctgtt
     1981 cgaaacgatg gggcagattc aggccaaaat cccaaccgag gtgctggtca ccgatcgccg
     2041 cgaatttgaa ctggctgaag agggttttat caccctgacc atgcgtaaag actctgataa
     2101 tgcagccttt ttctctgcaa actcggtaca aaaaccgaag cactttccgg gaaaagatgc
     2161 agaaaccaac tataagctgg gtacgcagct tccttatctc ttcatcatca accggttagc
     2221 gcactacatc aaggtgttgc agcgtgaaca actggggtca tggaaagaaa ggagcgactt
     2281 agagagggag cttaatacct ggatccgtca gtatgttgcc gaccaggaaa acccgcctgc
     2341 ggacgtgcgc agccgtaaac ctctgcgcgc agccagagtt gaggttatgg atgtggaagg
     2401 cgaacccggc tggtaccagg ttgcgctaag cgtgaggcct cattttaaat ttatgggggc
     2461 aaattttgag ctttccctgg ttggccggtt agacagggag taacacacca tgataccgga
     2521 tcgggaaaga accacaggtt ctctttttga gcgtatggaa gcctcttcgg ccagaaaccg
     2581 gcagggaggc agtattcatt cgctcaggca atccattcgc cagaacctac gaaacattct
     2641 taatacccgg tccggtagtt gccggggtgc gcctgagctg ggcattgatg aaccggaagg
     2701 ggctgaaaac ttccgcgaat ccatgagtcg ggccatcgag caatgtatag aacgctatga
     2761 accccggatc tcacacgctg aagtgcaggc agtggtttca tccgcctcca gcccgctgga
     2821 tatgacgttt catattacag cctgggtgac atttaacgaa acgcatgagg tgcttgaatt
     2881 tgatatggca ccgaatggca gccagcatta ccgggtggac tgacaaatgg aatttgaaga
     2941 acgctatttc cgggaagaac tcgattacct gcgccagctt agcaagctgc tggcaacgga
     3001 aaaaccccat ctggcccgct tcctggccga aaaagatgcg gatccggata ttgaacgcct
     3061 gctggaaggg gtggcttttc ttaccggcaa tctccgccag aaaattgagg atgaattccc
     3121 agaactgacg cacgggctta ttaagatgct atggcctaat tacctgcgtc cggttccggc
     3181 aatgaccctt attgaatata cgccggatat ggataagtct tctgtaccgg tgttaatccc
     3241 ccgtaatgag cagtttacaa ccaacgccgg ggaaatcaga gttgatgaag tgctgccctc
     3301 tgatgctaaa aaggaggagc cgcctccctg taccttcact ctctgccggg atatctggct
     3361 gctgcccgtt cgcctggagc agattgaaaa ccgcagtacg acccgtaatg gtgttatcaa
     3421 catcaccttt tcggtcgcac cgggaacgga cttccgcacg ctggatctga acaaacttcg
     3481 cttctggctc ggcaatgacg acaactatac ccgtgaccag ctttatttat ggttctgcga
     3541 atacttgcag ggtgccgacc tgactgtggg tgaacagcat attcgcctgc ctgagtttat
     3601 gctaaaagct gtcggttttg agccgcagga tgccatgctg ccctggccga aaaacgtcca
     3661 cagcggctac cggatccttc aggagtattt ctgttacccc gatgcgtttc tcttttttga
     3721 tctttgtggt tgtccggctt tgcctgacgg attgcaggcg gaattcttta ccctgcaact
     3781 gcgtttttcg cgccctttgc ccgtggacat ccggctgcgc cgcgattccc tgcgcctgta
     3841 ttgcgcacct gccattaatt tatttatcca ccatgcagaa gccatcacgc tggacaaccg
     3901 gcgggcagac tatccgctgg ttcccagccg ccattaccca caacattacg atgtattttc
     3961 cgttaacagt gtggtgagcc aggtccagga tatgttcagg aaaaaagatc tggggcgtcc
     4021 tgtttcgacg caggccgcgc gccagtggcc agcctttgaa agtttcagcc atcagatgga
     4081 atacagccgg aagcgggaag tggtgtactg gcatcaccgg accaaaacat ccctgttcca
     4141 tcgcggcttt gatcataccc ttgcctttat acatgctgat ggcagttatc cgtcagacga
     4201 atctctgctc agtaatgaag tggtttcggt atcgctgacc tgtaccaacc gtgagcttcc
     4261 gtcacaaata cgttccggcg atatcaccgg cacaaccggt aaaaatgcgg ctgttgcttc
     4321 atttcgcaac attacccgcc cgacgcaacc actctggccg gtcattgatg gcagcctgca
     4381 ctggtcccta ctctccgcca tgaacctgaa ttatctgtca ttactggata cggacgcgct
     4441 gaagcaggtc atcgccaact ttgatcgcca cgcaatccat catccgcaga cggcacggct
     4501 gtcacaacaa aagctggatg ccattgagcg tctggagacc cgccccgttg atcgcctgtt
     4561 tacgggtatt cccgtccggg gactggcctc cacgctgtat ctgcacccgg agccgtttgt
     4621 ctgtgaaggg gaaatgtatc tgctcggtac ggtgctttcg cattttctgt cgctgtacgc
     4681 cagcgttaac tcattccaca tgctgaccgt tgtgaacaca gaaagccagg agacatggaa
     4741 atggacggaa agaatcgggc agcatcctct tatctgagtc cgggaaatcc cccggcagat
     4801 aaagagcaga atgatccgct ggcacaggtt ttccataatg cctgctctta caattttttt
     4861 gcgatggcgg agctgttgca ccgcctggca aagggtgaaa aggggacgcc agaattatcc
     4921 ctgcgtgacg atccggcaca ggaaaccctg cgtttcagcg ccgatgccag ccttgccttc
     4981 ccgtgcagtg atatcagtgc actgaaacgg gatacgtcag gggcattcag gatgaccacc
     5041 acctttatgg ggttacaggg gagccagtcg cctctccccg gttattacct cgatcacctg
     5101 gcctggaaag cggtacatga acaaagtccg gtaggtgatt ttctggatat gttcagccac
     5161 cgcctgaccc agtttgtctg gcatatctgg cgtaagtacc gctatcacat cagttttcgt
     5221 aacggcggtg tggatgcgtt ctcgcagcgc atgtactccc ttgtcgggct gggccaccgc
     5281 cagcttcgcg ataagctggc gattaatcac agcaaaatgc tggcgtattc cgggatcctg
     5341 gcaaatccgg gacgatcccc ggaaataatc tgtggcctgg tgtcccactg ctttgatttg
     5401 tcagaagtga cgctgcaaaa ctggcaacgt cgcaaggtgg atattgaacc ggatcagcaa
     5461 aatagtctgg ggagctattc tcttaaaaat ggcgaaaaac tggcgggaag atcggtactg
     5521 ggcaactttg ttctgggtac acgagtgccc gagctcagcg gaaaattcca gctcagtatt
     5581 accagcctga cccgaaaaca gttcctctct tttctgccgt ccggcgaaaa cttcctgcca
     5641 ctgacgatgt tcgtgtcctt catcctgcgc gatcagcttg cctgggactt acatctgggg
     5701 ctggcgccgg agcaggtggg cgcaatgcgc ctgggcgata acaaaagtgc gctgctgggc
     5761 tggaccagct tcctcggcac accggaagaa cgaccctcag tcaccatcag ggtacggtcg
     5821 taaattatga taatcacgga gcagaatatg ccggaagaga aactacagac gctttcattg
     5881 caggtcatta acggcagtga gctggaaagc ggacgggcgg cgcgctgtct gttcacacag
     5941 cagggaaatg tcggccatgg ccccgaatgc cactggtcgg tacaggatcg tcagcagagc
     6001 attccggccc aggcttttac cgttatcctg cacgatggca cattttgtct gcgcccgcag
     6061 acggcacaac tgtggctgaa tcaggcaaaa gtcacagcaa catcagacct aatacagttg
     6121 cgccagggcg atgagatcca gatcggacgg ctgatggtga gggttcatct gaaccgggga
     6181 gatattcccc attacgatga ggaaatggcc actcccgaaa ccatcgttac caatcgcgat
     6241 atgctcacgg ataccctgct atcaacggag ggtacgccac actatccggg aatgactcac
     6301 cggcaccaac ttgcagacac cgtggtaaac ggtttttctg ccgatccact ccaggcactt
     6361 cagtccgaaa gcctgattac cacgggcgat ccgctttcag gcattgcggc tgtccggcca
     6421 tcggcaccgc tgtccgatcc ggcaagtaat ggggggatca atactccgtt tatggatctg
     6481 ccgcccattt atgccagccc cggcgatcgt aatgacgacg tctctgcggc agaaatggcg
     6541 caacgccacc ttgcggtcac ccccttactg cgcggtcttg gcggctcgct taccatgagc
     6601 aattccgacg atgcggatga ttttctggag gaggccggac gaacgttaca ggccgcaata
     6661 aaaggtctgc tcgatttgca gcagcagcgt aacagcctct cagacaaaca tttgcgcccg
     6721 ctggaagata acccgctgcg cctgaacatg gattacgcca ccgcgctcga cgtaatgttt
     6781 gccgaaggta aaagcccggt acatctggcg gctcccgccg ccgtcagtga aagcctgcgc
     6841 aatgtccgcc accacgaaga agctaaccgg gcagcgattg tggagtcgct tcgtgtcctg
     6901 ctggatgctt tctcaccaca aaatctgctg cgccgctttg tgcagtaccg ccgcagccat
     6961 gaactgcgcc agccgctgga tgatgccgga gcatggcaaa tgtacagcca ttattacgaa
     7021 gaactggcct ccgatcgcca gcaggggttt gagatgctgt ttaacgaggt ctacgcccag
     7081 gtctatgacc gggtgcttcg tgaaaaacag cgggagccgg aagcatgaac aggaaggcgt
     7141 ttctggcctg cgtgctgatg tgcgtcatat taaccggctg cgaaacggcg aaaaaaatca
     7201 gtgaggtgat caaaaacccg gatatccagg tcgggagcct gaaggagcaa ccttcggaaa
     7261 taaccgtcac cctgctgacc gaaccggaca ccaataccaa tgcggaaggt gaatcagccg
     7321 ccgtggatgt gcaactggtg tacctgactg acgactcaaa attgctggca gccgactatg
     7381 accagatagc cagtacgccg ctaccggatg tgctgggtaa aaactatatc gaccaccagg
     7441 atttcaacct gttgccggac accatcaaaa ccctgccgcc ggtaaagctg gatgagaaaa
     7501 cgcagtttat tggtgtcgtg gcctatttct ccgatgacca ggccactgaa tggaaacaaa
     7561 tcgagacggt ggaaggaacc ggccaccatt accgcctgct ggtgcatgta cgtcaaagca
     7621 gcatcgaaat gaaaaaagag gatgaataat tatggcaacc acccgcaaca aggtgatgtg
     7681 gcaggaaggg atgctgatgc gcccacacca tttccagcaa cagcagcgtt acaacgacta
     7741 cctggataac cagcgtttcc gggccatgaa tgatttatcc tggggattta ccgaactcac
     7801 cctcaacaat gaactgctgg cgcagggtaa gatcatgatt gacagcgcgt caggcacact
     7861 gcccgacggc accgtctttt ctatccccga ccaggacgca ctgcccgatc cgctgcaccc
     7921 gcaaaatttt ccggacgaaa gaagccgcaa tatctacctc gctctgccgg tcgccagtga
     7981 tgtgagaaat gaaatcagcg acggacggcg aatcgggcgt taccggctga attatgccga
     8041 tgtccgggat ttgcattcag aagaaggtga cgcgcgaaca ttgacgctgg gtcaactgac
     8101 gccgcgcatt atgagcggtg cagaagatat gagcgcctat attacgctcc cgctttgccg
     8161 tatcagcgat cgccatgccg atggctccct gacgttggat gacgatttta tcccctcctg
     8221 ccagaatatt caggtcagta agaaactgcg tgtttatctc aaagaggtac agggggccat
     8281 tggcggacgg gcaagcgatc tggcaaaccg tattggctct ccggcgcaga gcggcatcgc
     8341 ggatgtggcg gaatttatga tgttgcagtt acttaaccgt aaccagaccc ggtttaccca
     8401 tcgcgcccgc cgatcccagc tccacccgga agatttctac cttgatcttg ctgggttgct
     8461 gggtgaactg atgaccttta ccgagccgtc gcgcctgccc tgcccgcttg atgtgtatga
     8521 tcatcatgat ctgaccaaaa catttaaaac actgttaccg gaagtcaaac gggcgctaca
     8581 taccgtactg tcgccaagag cggtcaatct gccgctgcat ctgcgtgacg gtatctggca
     8641 ggccgatatc catgacacag aactgctgca atctgccacc tttgtgctgg ccgtggcggc
     8701 aaacgtgccg gtcgatcaga tccagcgtca gtttatccag cagtcgaaaa tttcctcgcc
     8761 ggaaaaaatc cgcaatatgg tcagtgtgca gatccccggt attccattgc gtgccattat
     8821 ggtggctccc cgccagcttc cttatcattc cgggttcagc tatttcgaac tcgacaagag
     8881 cggacaggcc tggacagaaa tggctgccgc cggggccgtt gcactgcatg tatccggcag
     8941 tttcccggat ctgaacatgc aactgtgggc gataagaggg taagacgatg gatgaggggt
     9001 cactgtctct gccaccattt accggatacg acgaaaaaag ccagcgcaac taccatctcg
     9061 cgcttcgtgg caacagcctg aatccgatga tcgacgcggc cacgccgcta ctcggtatgg
     9121 taatgcgcct ttcaaccatg aacagccaga cgatgccgga gcatcttttt gctcaggtgg
     9181 tgacggatgt tcaggccgtg gagcaactgc ttcaggagca gggctacgaa ccgggcgtga
     9241 ttatctcgtt tcgctacatc ccctgcacgt ttattgatga agcggcgctc ggtaacggct
     9301 ggtcaaacaa gaacgagtgg atcaagcagt cattgctggt ccatttccac aacgaagcct
     9361 ggggtggaga gaaagttttc atcctgctgg agcgcctgat ccgtgaaccc aaacgttacc
     9421 aggatctgct ggaatttctc tggatctgtt tttctctggg atttcgcggg cgctacaaag
     9481 tggcagcaca ggatcagggg gaatttgagc agatctaccg ccgtctgtac cacgttctgc
     9541 ataaactgcg cggcgatgcc cctttcccgc tgttacacca ggacaaaaaa acccagggcg
     9601 ggcgttatca gctcatcagt cgcctgactg ttaaacatat tttttgtggt ggcgttgtcg
     9661 tgctggcgtt gttttacctg ttctatctgt tgcggctgga cagccagacg caggacatcc
     9721 tgcaccagct aaacaaatta cttcgataag gacgtttatg atccagattg atcttcccac
     9781 gctggtaaaa cggctgaacc tgttctcccg ccaggcgctg gagatggccg cctctgaatg
     9841 tatgagtcag caggcagcgg aaattaccgt cagccatgtg ctcattcaga tgctcaccat
     9901 gccacgcagt gacctgcggg ttattacccg ccagggcgat attggcatgg aagagttgcg
     9961 ccaggcgctg acggtagaga actacacaac cgcccgttct gcggacagct acccggcgtt
    10021 ttccccgatg ctggttgagt ggctgaaaga gggctggctg ctggcgtcgg ctgagatgca
    10081 gcacagcgaa ctgcgcggcg gcgtgttgct gctggccctg ctgcattcgc cgctgcgtta
    10141 tataccgcct gctgccgccc ggctgttgac cgtgaccgtc tgcaacagga ctttgtgcag
    10201 tggacacagg agtcggcgga atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca
    10261 atgacggacg cctctgacac cctgcttgcc cgctatgcca aaaacatgac cgcagacgcc
    10321 cgtaacggca ggcttgaccc ggtactgtgc cgcgaccacg aaatcgacct gatgatcgac
    10381 attctctgcc gccgccgtaa aaacaacccg gtggtggtgg gcgaagcggg cgtgggcaaa
    10441 agcgcactga ttgaagggct ggcgctgcgc atcgtggcag gccaggtgcc ggacaagctg
    10501 aaaaacaccg atatcatgac ccttgacctg ggcgcattgc aggccggggc gtcggtgaag
    10561 ggtgaattcg aaaaacgttt caaagggctg atggcggagg tcatttcctc cccggtgccg
    10621 gtcattctgt ttatcgacga agcacatacc ctgattggcg cgggcaacca gcagggcggg
    10681 ctggatatct ccaacctgct caaaccggcg ctggcgcgcg gcgagctgaa aaccatcgcc
    10741 gccaccacct ggagcgagta caaaaaatac ttcgaaaaag atgccgccct gtcgcgccgc
    10801 ttccagttgg tgaaggtcag cgaacccaac gctgccgaag ccaccattat tctgcgcggt
    10861 ctgtcggcgg tctatgaaca gtctcacggc gtgctgattg atgatgacgc cttgcaggcc
    10921 gctgcgacat taagcgagcg ttatctctcc gggcgtcagt taccggacaa agcgattgat
    10981 gtgctggata ccgcctgcgc ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc
    11041 tcggcgctga ccactctgag ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag
    11101 cttcgcatcg gactgcgtac cgacacatca cggatgaccg aggtgctggt gcagtatgat
    11161 gaaacgctga cggcgctgga tgaactggaa gcggcctggc accagcagca gacgctggtc
    11221 cgggagatta ttgcgctgcg ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg
    11281 ttgccggacg cagataccgt ggaggatacg cagccagagt cagagtcaga acaggataat
    11341 accggtgccg taccggctga tgaggccgac agagaacagc cggaagagac cgctgaaaca
    11401 gtttccccgg tacaacgtct ggcacatctc actgccgaac tggacgccct gcataacgac
    11461 cggttgctgg tttccccgca cgtcgataaa aaacagattg cggcggtgat tgccgaatgg
    11521 accggcgtgc cgcttaaccg cctgtcgcag aatgaaatgt cggtcatcac cgacctgcca
    11581 aaatggctcg gcgacaccat caaaggccag gacctggcga ttgccagcct gcacaaacac
    11641 ctgctgaccg cacgcgccga cctgcgccgt ccgggacgcc cgctgggcgc gttcctgctg
    11701 gctggcccca gcggcgtggg taaaactgaa accgtcctgc aactggcaga gctgctctac
    11761 ggcggtcgcc agtacctgac caccatcaat atgtccgagt tccaggagaa acataccgtc
    11821 tcgcgactga ttggttcgcc tccgggctac gttggctacg gtgaaggcgg cgtactgacc
    11881 gaagcgattc gccagaaacc ctactcggta gtactgctcg atgaagtgga aaaagcgcac
    11941 ccggatgtgc tcaacctgtt ctaccaggcg ttcgataagg gcgaaatggc agacggtgaa
    12001 ggccgcctga ttgactgcaa aaatatcgtc ttcttcctga cgtccaacct cggctaccag
    12061 gtaatagtcg agcatgccga tgacccggaa accatgcagg aagtactgta tccggtgctg
    12121 gccgacttct tcaaacctgc cctgctggcg cgtatggaag tggtgccgta cctgccgctg
    12181 tcgaaagaga cgctcgccac catcattgcc gggaaactgg cccgcctgga taacgtgctg
    12241 cgcagccgct ttggcgcgga ggtgattata gaaccggaag tgacggacga aatcatgagc
    12301 cgcgtcaccc gcgcggaaaa cggcgcgagg atgctggaat cggtcatcga cggcaatatg
    12361 ctgccgccgc tctccctgct gctgttgcag aaaatggcgg cgaatacggc ggttgcccgg
    12421 attcggttgt cggcagtgga cggcgcattt acggcagacg tggaagatgc tcagaacgac
    12481 gagtccgtca caaaggatga aacggtttta tgagcaggtt gcgcgttttc cggcagacag
    12541 gatggtttat cgcgggcctg atgacgggct tacccgccac tgccgcgccc gcagaagcat
    12601 cgtcaatggc cggtgtggcg gtcgccgtcg ccaccacgac cccgccggat gcgacagcca
    12661 cgttacaggc catgcagtcc tgtcggcggg agtccgcggc gctggagcgg cttgactgct
    12721 atgaccgcct gctggcaccg ctgtccccgt cagggtttga cggggcgctg gtcaaagccg
    12781 gttttgtggg cgaagcctgg acccgcgcca cggaacagga aaaacaccgc cagggcaaca
    12841 ccacagagct gctggtgacg caggtgccgg gggagcgccc gacggtggtg ataaccaccc
    12901 cggctatcgg tcatgttccg ccacgcccgg tgctgatgtt cagttgcgtg gacaatatca
    12961 cccggatgca ggtggcgctg atgcaccctc tggatgtcca tgatatcgcc gtcactctga
    13021 atgccgacaa ccgggcattg cgcagccact ggtttgtgcg cgagaacggc accctgctgg
    13081 agtccagccg ggggttatcc gggattgatg aaattaagca actgttcggc gcaaaaacgc
    13141 tgacggtgga taccggcgca gacaatgccg ccggaaagct gacctttaac attgacgggc
    13201 tggcaagggc gattgctccc ctgcgcgatg cctgccactg ggcgggagaa taaaaatatg
    13261 gcaatggact tacgcgatcc gaatgtctgg atatcgcacc tgctggaaaa cctgccggaa
    13321 gagaaactgg catcggcgct gaaagatgac aacccgaact gggagtatat cgacggcgaa
    13381 atcgtcaagc tgggatcgct ggcccacagt caactggata tcccggaact ccagcgccgg
    13441 gggctggtca ttctggcttc ggagagtaag gattttcgtc tgctggcgca tctgctgaga
    13501 accctgcaac acgccggtga tcctcatctc gcattgcgcc tgctggcgct gtatgtggag
    13561 cattactggg cggtggctgc gccgcagaat atggcgcata aaaaacgctt tgccagccag
    13621 gtcattaaac gctttgaaac aggtattgaa gttttttcac agaacgccgc cacagcgcag
    13681 cgcgatgcgc tgtcagggga actggcaaaa ctggcgcaat gctggcagtc acataacgcc
    13741 ccggaactgg cacaggctac tgatgatctt tttgccctgt accagcgtgc gtttaatcgt
    13801 gcggcaccgg ctcctgtccc cactccggcg gcctccggta gttcaccaca gaccaccgcc
    13861 acgtctgaaa gcggcgtgac gcaacccagt gctccggctc cccaaatcgt catcgacagt
    13921 cacgacgaca aagcctggcg cgacacgctg ttaaaagtgg cggccatttt atgtgaacgc
    13981 cagccggatt caccgcaggg ctaccgtctg cgccgccatg cgctttggca gaacatcacc
    14041 agtacgcccc aggcggaaag cgatggccgt actccactgg ctgcggtctc cgccgatatg
    14101 gtggcagatt accatgctca gctcggtagt gccgatatgg cgctgtggca gcaggtggag
    14161 aaaagcgtac tgctggctcc ttactggctg gatggtcact gcctttctgc acagacggcg
    14221 ctgcgtctgg gttacaaaca ggtggcagac gccatccgcg atgaggtcat ccgcttcctt
    14281 gagcgtctgc cccagcttac cgggctgctg tttaatgacc acacaccgtt tatcagtgag
    14341 cagacgaaac aatggctggc agcttcgccc gacgccaaag ttgcaccggt tgcgcaaatc
    14401 ggtgaggaat cgaaggcagc cagagcctgt tttgctgagc agggtctgga ggcggcgctg
    14461 cgatatctgg acatgctacc cgaaggcgat ccccgcgatc agtttcaccg ccagtacctt
    14521 gccgcacagt tgacggaaga ggcggggctg gtacagcttg cgcagcagca gtaccggatg
    14581 ttgttcagga tgggattgca gatgatggtg gcagactggg agccgtccct gcttgagcag
    14641 ctagaacaaa aattcacggc agaacaataa aagagaacag gagtttttgt gttcagatta
    14701 ccaacacccc gactactcag cggactcaaa tcagccctgc gaccggcgat gccccggttt
    14761 aaagtctctg ctttctggct gctgatactg gcgtggattt ttctgctggt gtggatctgg
    14821 tggaaaggcc cgacgtggac gctgtatgaa gaacagtggc tcaaaccact ggcgaaccgc
    14881 tggctggcaa cggcggcgtg ggggattatt gccctgatgt ggcttaccgt ccgggtgatg
    14941 aagcgcctgc aacagcttga aaaacagcaa aaacaacagc gcgaggaagc cattgatccg
    15001 ctcagtgtgg aactgaacgc ccagcagcgt tacctcgacc gctggctgct gcgcctgcaa
    15061 cgccatctcg acaaccgccg tttcctgtgg caattgccgt ggtatatggt catcggcccg
    15121 gcgggcagtg gcaaaacgac actgctgcgc gaggggtttc cgtcagacat tatttatgcc
    15181 ccggagggct cgcgcggcgc agaacaacgc ctgtacctta cgccccatgt cggtaaacag
    15241 gcggtgatct ttgatatcga cggcacactc tgcgctcccg ctgatgcgga tatcctgcat
    15301 cgccgtctgt gggaacatac cctcggctgg ctgaaggaaa agcgcgcgcg ccagccgctg
    15361 aacgggatta ttctgacact cgatttaccc gatctgctga ccgcagacaa gcgccgccgt
    15421 gagtatctgc tacagacgct gcgcagccgc ttgcaggata tacgccagca tcttcactgc
    15481 cagctaccgg tttacgtggt actgacccgg cttgatttat tgcagggttt tgccgccctg
    15541 ttccagtccc tgaacagaca ggatcgtgat gcgattctgg gtgtcacgtt cacccgccgt
    15601 gcccatgaaa atgatgactg gcgaacagag ttgaatgctt tctggcagac atgggtggat
    15661 cgaatgaatc tggcgttgcc ggatctgatg gtcgctcaga ctcacacccg cgcgtcttta
    15721 ttcagttttt cccgccagat gcagggaagc cgtgaacccc tggtgtcact gcttgaaggt
    15781 ctgcttgacg gcgaaaatat gaacgtgatg ctgcgtggtg tctatctcac ctcgtcactg
    15841 caacgcggcc agatggatga catctttacc cagtccgccg cccgccagta ccggctgggc
    15901 aataacccac tggcgtcctg gcccctggtg gacaccgcgc cttatttcac ccgcagcctg
    15961 ttcccgcagg cattactcgc agagcctaat ctggcaacgg agagccgtga ctggctgatg
    16021 cgttcccgtc gccgcctgac ggttttctct gccacaggcg gcgtggcggc actgctgctc
    16081 atcaccggct ggcatcacta ttacaacggt aactatcagt ccggcatcac cgtgcttaag
    16141 caggccaaag cctttatgga cgtgccgcct ccgcagggga aagacgacta cggcaacctg
    16201 caactgccgc tgctgaaccc ggtacgcgat gccacactgg cctatggcga ctggggcgac
    16261 cgcagccgtc tggccgatat gggactgtac cagggacgac gtatcgggcc ttatgtggaa
    16321 cagacctatc tgcaactact agagcagcgt tacctgccct cgctgtttaa cgggctggtc
    16381 aaagcgatga acgccgcgcc gccggagagt gaggaaaaac tcgccgtgct gcgcgtgatg
    16441 cgaatgctgg aggacaaaag cggacgcaat aatgaggtgg tgaagcagta tatggcaaaa
    16501 cgctggagcg aaaagttcca cggccagcgt gatatccagg cacaactgat gtcccatctt
    16561 gactacgcgc tggctcatac tgactggcat gcagagcgtc aggcgggcga cggtgacgcc
    16621 atcagccgct ggatgccata tgacaagccc gtggtatcag cacagaaaga actgagcaaa
    16681 ctgcctgtct accagcgggt ttaccagagc ctgaaaacgc gggcgctggg cgttctacct
    16741 gctgacctca atctgcgtga ccaggtaggg ccaacctttg accaggtctt tacatctgcc
    16801 gatgacaaca aactggttgt tccacagttt cttacccgtt acggcctgca aagctatttt
    16861 gtaaaacagc gcgatgaact ggttgaactg acggcaatgg attcctgggt actcaacctc
    16921 acccgcagtg tgaaatacag tgacgccgac cgcgcggaaa tccagcgcca gttgaccgag
    16981 cagtatatca gcgactacac cgctacctgg cgagccggga tggacaatct gaatatccgc
    17041 aattttgagt ccattggaca actgaccggg gcgctggagc aggttatcag cggcgaccag
    17101 cctttgcagc gggcgctgac tgtgttgcgt gacaacacac agccaggcgt cttttctgaa
    17161 aaactttctg ccaaagaacg ggatgaggct ctggcagaac cggactacca gttgctcacc
    17221 cgcctcgggc atgaatttgc cccggaaaac agtactctgg cagtacagaa agacaaagaa
    17281 agcacgatgc aggccgtgta tcagcaactc accgagttgc accgctatct gctggcaatt
    17341 cagaacgcgc ctgtaccagg gaaatcggcg ctgaaagccg tgcagttgcg gcttgatcag
    17401 aacagcagcg atccgatatt cgccacccgc cagatggcaa agacactgcc tgctccgctc
    17461 aaccgctggg ttggcagact ggctgaccag gcctggcatg tggtgatggt ggaggccgtt
    17521 cactatatgg aagtggactg gcgcgacagc gtggtgaaac cgtttaacga gcaactggca
    17581 aataactatc cgtttaatcc gcgttctgca caggatgcct cactggatgc cttcgaacgc
    17641 ttctttaaac cggatggcat actggatacc ttctaccagc agaacctgaa gctgtttatc
    17701 gacaatgacc tgagtctgga ggatggcgat aacagcgtca ttattcgcga agatattatt
    17761 gcgcaactga aaaccgcgca gaaaatccgt gacatcttct tcagtaaaca gaacggtctg
    17821 ggaacatcct ttgccgtgga aacggtatcg ctctccggca acaaacgccg cagtgtactg
    17881 aaccttgacg gccagttagt cgattacagc caggggcgca actataccgc ccatctggtc
    17941 tggcctaaca atatgcgcga aggcaacgaa agcaaactga cgctcatcgg caccagcggc
    18001 aacgcaccgc gcagtatcag cttcagcggg ccgtgggcgc agttccgcct tttcggggcc
    18061 ggacaactga ccggagttca ggatggcaac tttaccgtgc gctttagcgt ggacgctggc
    18121 gcgatgacct accgtgtgca taccgacact gaggataacc cattcagcgg tgggttattc
    18181 agccagtttg gtctgtcaga cacactgtac tgaggaggca gggatggata gtaacggact
    18241 gacacaaact atcgttaccg gcagtgaccc gcgcgggctg ccggaattca gtgccatccg
    18301 cgaggaaata aacaaagcca gccacccgtc acagcctgag ctgaactgga aactggtgga
    18361 gtcgctggca ctggcgattt ttaaagccaa cggtgtggat ttacacaccg ccacctacta
    18421 tacgcttgcc cggacacgga cacagggact ggcgggattc tgcgaaggtg cggaactgct
    18481 ggcggcaatg gtaagccacg actgggataa gttctggccg cagggcggcc cggcgcgtac
    18541 tgaaatgctg gactggttta actcccgaac cggcaatatt ctgcgtcagc aaatctcctt
    18601 tgcggaatcc gacctaccac tgatctaccg cacagagcgg gcattgcagc ttatctgcga
    18661 taagctccag caggtggaac tgaagcgcgt tccgcgcgtg gagaatctgc tctattttat
    18721 gcagaacacg cgtaaacggc ttgaacccca gctgaagagt aacactgaga acgccgcaca
    18781 gaccacggtc agaacgctga tttatgcccc ggaaacacag gcatcttcca caccagaagc
    18841 ggtagtgcct cccctgcccg gcctgcctga gatgaaagtg gaagtgcgca gtctgacaga
    18901 gaatccccca caggccagtg tgataaagca aggcagtacg gtaagagggt ttatcgcagg
    18961 gatcgcctgt tcagtggctg tcgcctcagc attgtggtgg tggcaggtct atccggtgca
    19021 gcagcaactg ttacaggtta acgacaccgc tcagggcgca gcaacggtgt ggatggcctc
    19081 acctgaactc gaaaactatg agcgcaggct gcaacaactt cttgatacct ccccggtaca
    19141 gccgctggaa accgggatgc agatgatgcg tgttgccgac agtcgctggc cggaaagcct
    19201 gcaacagcaa caggcctcga cacaatggaa tgaggcactc aaaacccgcg cacagagtag
    19261 cccgcagttg cgtggctggt tgcagacccg ccaggactta catgcttttg cagatctagt
    19321 gatgcagcgc gagaaagagg gactaaccct ttcctatatc aaaaatgtca tctggcaggc
    19381 ggagcgggga ctggggcagg aaacacccgt tgagtctctg ttgacgcagt accaggatgc
    19441 ccgtgcgcag aagcagaata cagatgcgct ggaaaaacaa attaatgagc gactcgaagg
    19501 cgtgttaagc cgctggctgc tgctgaagaa taacgtcatg ccagaggcgg caaccggtac
    19561 cacggccgaa aaataattat ttaattaaag gagattatca tggcaaatat aagttattta
    19621 tctttgagtg gagagacaca agggctgatt tcagcaggtt gctcaacgct tgattctgtc
    19681 ggaaacaaag cgcagccaga acataaagat cagatcatgg tttatgcgtt aatgcattcc
    19741 atctcaagaa gtcaaaatgt caatcatcat gaattaatta tcaccaaacc ggttgataaa
    19801 tcctcaccat tgctggcaaa agcacttagt gacaatgaaa aaatggcaat atgtgaattt
    19861 atactctacc gaaccagtaa agcgggtatt taccagcctt attataaaat aaatctgtca
    19921 aaagcccgaa tatcaagtat tgattttgtc acaccgcatg ctgtcctgga aaaagaactg
    19981 gaaccacagg agagaattgc ctttatttat gaagatattt cctgggagca tacgttagcc
    20041 ggtacgaatg caatgagtaa atggcaggat cgtgtacagt aacttactgt aataacataa
    20101 taaattacta ttgcggagag ataaaatggc tggtttaagg aataacaaca acacacagaa
    20161 tgcacagtgg gctgattatg tgggtgacat tcttcggggg gctcagccga taaaccagct
    20221 agtacctcag cacccttatc ttaacgacgt cccactcatt gatgagttaa ggcatcagaa
    20281 cactcatcat gttattctct tgacacttga tgttgcaaaa aaaatactca gcccaataac
    20341 atcatttgat tacattcatt ttatcactac acatccttca ggtataaaag atacactcgc
    20401 ctggttagtt aacgcgggta aattaatgac tgagtttgat gacaatggga aaattatatt
    20461 taaccttaat gccttaaagt atacaaaagc aagttacttt gaaatacttg gagagaaata
    20521 tataaaaatc acaacatcca gcccctggct tttagaaaag ctcggtaaat atattttctc
    20581 ttcacgagcg cctcaagttc tggaactcgc tattggctgg cgaggagctt tatctgaatc
    20641 aataaaagga gttaagttct gtatctggtt ttcagtagcc tggagaacca tagaatttat
    20701 tatgagttca gagcgagatc tggttaattt tcttggcgat ttttcaatgg atgtggccaa
    20761 agcagttata gccggtggtg ttgcaaccgc tattggaagc ctggcttctt ttgcctgtgt
    20821 tagctttggc tttccagtaa ttcttgtcgg aggagcaatt ttactgacag ggatagtgtg
    20881 tacagttgtt ttaaatgaaa tcgatgctca atgccattta tcagaaaaat taaaatatgc
    20941 aattagagat ggactaaaac ggcaacagga acttgataaa tggaaaaggg aaaacatgac
    21001 tccatttatg tatgttctta acactccacc cgtgatatga ggattaacca tgcgtttaca
    21061 tgt
//
LOCUS       DAZFXT010000079        20819 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.079, whole genome shotgun sequence.
ACCESSION   DAZFXT010000079 DAZFXT010000000
VERSION     DAZFXT010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.079"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(124..1773)
                     /gene="actP"
                     /locus_tag="ACHKW0_003152"
     CDS             complement(124..1773)
                     /gene="actP"
                     /locus_tag="ACHKW0_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015123 - acetate transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015370 - solute:sodium symporter activity
                     [Evidence IEA];
                     GO_function: GO:0031402 - sodium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HFO7475940.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1770..2084)
                     /gene="yjcH"
                     /locus_tag="ACHKW0_003153"
     CDS             complement(1770..2084)
                     /gene="yjcH"
                     /locus_tag="ACHKW0_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HFO7475941.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2284..4242)
                     /gene="acs"
                     /locus_tag="ACHKW0_003154"
     CDS             complement(2284..4242)
                     /gene="acs"
                     /locus_tag="ACHKW0_003154"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0016208 - AMP binding [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HFO7475942.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4634..6070
                     /gene="nrfA"
                     /locus_tag="ACHKW0_003155"
     CDS             4634..6070
                     /gene="nrfA"
                     /locus_tag="ACHKW0_003155"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HFO7475943.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIEIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6115..6681
                     /gene="nrfB"
                     /locus_tag="ACHKW0_003156"
     CDS             6115..6681
                     /gene="nrfB"
                     /locus_tag="ACHKW0_003156"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HFO7475944.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASGDRYEVTQQRN
                     PDAACLDCHKPDIEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6678..7349
                     /gene="nrfC"
                     /locus_tag="ACHKW0_003157"
     CDS             6678..7349
                     /gene="nrfC"
                     /locus_tag="ACHKW0_003157"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HFO7475945.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLMIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7346..8302
                     /gene="nrfD"
                     /locus_tag="ACHKW0_003158"
     CDS             7346..8302
                     /gene="nrfD"
                     /locus_tag="ACHKW0_003158"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HFO7475946.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGVAVALIAMAIRQRKNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8322..10040
                     /gene="nrfE"
                     /locus_tag="ACHKW0_003159"
     CDS             8322..10040
                     /gene="nrfE"
                     /locus_tag="ACHKW0_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HFO7475947.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10033..10416
                     /gene="nrfF"
                     /locus_tag="ACHKW0_003160"
     CDS             10033..10416
                     /gene="nrfF"
                     /locus_tag="ACHKW0_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HFO7475948.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10413..11009
                     /gene="nrfG"
                     /locus_tag="ACHKW0_003161"
     CDS             10413..11009
                     /gene="nrfG"
                     /locus_tag="ACHKW0_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HFO7475949.1"
                     /translation="MKQSQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAQYQRQRD
                     PLHQFASQQTPEAQLQVLQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11351..12664
                     /gene="gltP"
                     /locus_tag="ACHKW0_003162"
     CDS             11351..12664
                     /gene="gltP"
                     /locus_tag="ACHKW0_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HFO7475950.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTIAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12742..13431)
                     /gene="yjcO"
                     /locus_tag="ACHKW0_003163"
     CDS             complement(12742..13431)
                     /gene="yjcO"
                     /locus_tag="ACHKW0_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YjcO"
                     /protein_id="HFO7475951.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13525..15672)
                     /gene="fdhF"
                     /locus_tag="ACHKW0_003164"
     CDS             complement(13525..15672)
                     /gene="fdhF"
                     /locus_tag="ACHKW0_003164"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(15253..15255),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HFO7475952.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15870..17336)
                     /gene="mdtP"
                     /locus_tag="ACHKW0_003165"
     CDS             complement(15870..17336)
                     /gene="mdtP"
                     /locus_tag="ACHKW0_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HFO7475953.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17333..19384)
                     /gene="mdtO"
                     /locus_tag="ACHKW0_003166"
     CDS             complement(17333..19384)
                     /gene="mdtO"
                     /locus_tag="ACHKW0_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HFO7475954.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEVRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19384..20415)
                     /gene="mdtN"
                     /locus_tag="ACHKW0_003167"
     CDS             complement(19384..20415)
                     /gene="mdtN"
                     /locus_tag="ACHKW0_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HFO7475955.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20434..20709)
                     /gene="ytcA"
                     /locus_tag="ACHKW0_003168"
     CDS             complement(20434..20709)
                     /gene="ytcA"
                     /locus_tag="ACHKW0_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtcA family lipoprotein"
                     /protein_id="HFO7475956.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4690 a         5512 c         5702 g         4915 t
ORIGIN      
        1 ttctacgact gtcaaaaatc gctcattttt taatgagttt atttgtttaa tattatggga
       61 aaaggtgatg catgttggag aggaagagta ttccccggtc aggcgaccgg ggaaggggag
      121 agattaatgt gcgcggcctt gctcaacgcc aaagccggtc tgggagcgga taaactgtgc
      181 acggaacagt tcacgctcgc gcgcaccttc cgctgagtta tcggttgccg agaagaacca
      241 gatgccgagg aatgccacgc tgatagagaa cagcgccggg tattcatacg ggaagatggc
      301 tttttcgtga ccaaggatct gtacccaaat cgtcgggccg aggatcatca gtaccactgc
      361 ggtaatcagc cccagccagc cacccatcat cgcgccacga gtggtcagtt tcgaccagta
      421 catagaaagc agaatgatcg ggaagttaca gctcgctgcg atggcaaacg ccaggcctac
      481 catgaaggcg atgttctgat tctcaaacag cacgccgagg ataatggcaa tcacacctaa
      541 aatcagcacg gtgattttcg atacccgcag ttcttcacgt tcggtcgcgc cttttttgaa
      601 gacgttagcg tacaggtcat gcgaaaccgc tgatgcgccc gccagcgtca gaccagcaac
      661 caccgcaaga atggtggcaa aagcaaccgc tgaaataaaa ccgaggaaca ggttgccgcc
      721 cactgcattc gccaggtgaa cggccgccat gttgttacca ccaatcagat ggcccgccgc
      781 gtctttatat tccggattcg caccaaccag catgatcgcg ccgaagccga taataaaggt
      841 cagaatatag aagtagccca taaacccggt ggcgtagaac acgctcttac gtgcttcgcg
      901 ggcatcgctg actgtaaaga agcgcatcag aatgtgcggc aagcccgccg taccaaacat
      961 cagtcccaga cccagagaga gcgcggagat cggatctttc accagcccgc ccggcttcat
     1021 gatgtcgaca cctttcgggt gtaccgccat cgcttcactg aacagattgt tgaagctaaa
     1081 gccgacgtgt ttcatcacca taaaggccat aaagctggca ccgaacagca acagcacggc
     1141 tttgataatc tgtacccaag tggtcgccag catgccgccg aacaggacgt acatcatcat
     1201 cagcacgccg accagcacca ccgcaatgtg atagttaagg ccaaacagca gctcgatcag
     1261 tttacctgcg cccaccatct gggcgataag gtaaagcgcc accaccacca gagaaccaca
     1321 ggccgaaaga atacgaatcg gcccttgttt cagacggtaa gaggccacat cggcaaaggt
     1381 gtagcgcccc aggttacgca gacgttcggc aatcaggaac aaaatgatcg gccagcccac
     1441 caggaagccc agcgagtaaa ttaagccgtc atagccggag gtaaacacca gcgcggagat
     1501 ccccaagaat gaggcggcgg acatatagtc cccggcaatc gccagcccgt tctggaagcc
     1561 agtgatattg ccgcctgcgg tgtagtagtc gctacgagaa cgtacgcgtt ttgatgccca
     1621 gtaggtaatg ccgagcgtaa acacgacgaa aatcaggaac ataataatcg cctgccagtt
     1681 cgttggctgg cgctctacgg ccccgctaat agcatccgcg gcgttagctg cgaaagggag
     1741 tgtggcggca agcgccgtca gaactctttt catgatgctt gtacctcatg caggacttca
     1801 ttattaagac ggtcgaattc gccgttcgcc cgccagatgt agataccggt gagaacaaag
     1861 gagatcacaa tcactccaac accaattgga ataccgcgtg tgacgctggt gttcggattc
     1921 agcggcgtgc ccagccagcc gggcgcgaag gcgatcagta aaataaagcc gatataaact
     1981 gccagcataa taatcgacag gatggtggca aaccgttgcc gtttttcgac taactccctg
     2041 aaatgcgcat tgtcttctat ccgctgataa atagtgccat tcatcacaga ttctccagag
     2101 gtaatgtagg gattgtttta attcccgctc ccttatggga gaaggttaac gctcgggtaa
     2161 cccttgccga atgtaggccg gataaggcgt ttacgccgca tccggcaatc aatgcctgat
     2221 gcgacgctgt cgcgtcttat caggcctaca aaccgttacc gactcgcatc cggcaattgt
     2281 gggttacgat ggcatcgcga tagcctgctt ctcttcaagc agcttctcga ctacgccagg
     2341 atcggcaagc gtcgaggtat cgcccaggtt gctggtatcg cccgccgcaa ttttgcgcag
     2401 aatacggcgc ataattttgc cggagcgggt tttaggcagg gagtcggtcc agtgcagcac
     2461 gtctggcgtc gccagcgggc caatctcttt acgcacccag ttgcggactt ctgcgtacag
     2521 ttctggtgac ggttcctccc cgtgattaag cgtgacgtag gcgtagatcg cctgaccttt
     2581 aatattgtgc ggaataccta ctacggcggc ttcggcaatc ttcggatgcg ccaccagcgc
     2641 cgactcaatc tctgccgtcc ccagacggtg accggagacg ttcagcacgt cgtccacacg
     2701 cccggtgatc cagtaatagc catcttcatc gcgacgcgcg ccgtcgccgc tgaaatacat
     2761 atttttgaag gtggagaagt aggtctgttc aaaacgttcg tgatcgccaa acagcgtacg
     2821 cgcctgaccc ggccaggagt cggtgatcac caggctgcct tcggtagcgc cttccagcgg
     2881 gttaccttcg ttatcgacca gcgccggttg cacgccgaag aacggacgtg ttgccgaacc
     2941 ggctttcagc tcggtagcgc caggcagcgg cgtgatcatg aaaccgccag tttcggtctg
     3001 ccaccaggta tcgaccaccg gacatttctc gttgccgatt tttttccagt accactccca
     3061 cgcttccggg ttaattggct cgcccacgga accgagaatg cgcagcgacg aacggtcggt
     3121 gccttcgatc gctttatcgc cttccgccat cagcgcgcgg atcgccgtgg gtgcggtata
     3181 gagaatattg acctgatgct tgtccaccac ctgcgccata cgggcaggcg tcggccagtt
     3241 gggtacgcct tcaaacatca gcgtggtcgc accgcaggcc agcgggccgt acagcaagta
     3301 actgtgtccg gtcacccagc ccacatcggc ggtgcaccag tagatatcac ccggatgata
     3361 atcaaagaca tatttaaagg tcagcgccgc gtacaccaga taaccgccgg tagtatgcag
     3421 cacacctttt ggcttaccgg tagaaccgga ggtgtagaga ataaacagcg gatcttcggc
     3481 gttcatctct tccgcctggt gctgatcgct cgcttgctca accaggtcgt gccaccacag
     3541 gtcgcgccct tcctgccagt caattttccc gccagtacgc ttcagtacca ccacatgctc
     3601 tacgctggtg acgttcgggt ttttcagcgc gtcatcaacg tttttcttca gcggaatact
     3661 gcgcccggca cgcacacctt cgtcggaagt gatcaccagt cgtgagttgg aatcaataat
     3721 gcgcccggca acggcttccg gcgagaagcc gccgaaaatc accgaatgca ccgcgccaat
     3781 gcgggcgcag gccagcatcg caaccgcggc ttccggcacc atcggcatat aaatcgccac
     3841 cacatcacct tttttaatgc ccagctcgag cagggtattg gcgaagcggc agacgtcgcg
     3901 gtgcagctct ttatagctga tatgtttgct ctggctggcg tcgtcgcctt cccagatgat
     3961 ggcggtgcga tcgccgtttt cttgcagatg gcggtcaagg cagtttgccg ccagattcag
     4021 cgtgccgtcc tcgtaccatt taatggacac attaccgggg gcaaaggagg tatttttcac
     4081 cttctggtac ggtttgatcc agtcgagaat ttttccctgt tcgccccaga aggtatcagg
     4141 tacgttaata gattgttgat acatcgcctc gtactgctga gggtttatca ggcaacggtc
     4201 tgcgatgttg gcaggaatgg tgtgtttgtg aatttggctc atgcttttgt tctccttgtg
     4261 ggatgttaat aatatgtggc ataagcgtta aatgtagggg tattggcagt tttgtttagt
     4321 atttgggcga cagatcacgc aaaagtagaa ttgtgcaaat aaacggcagg gtaatttttg
     4381 aaggtcacca acaaaagttg attaattctt tgaggaacat gcagttatgc atgctgttga
     4441 aaaagaggaa gatactgact aactctaaag tggtatttta catgcactta caattgatta
     4501 aagacaacat tcacagtgtg gttatttgtt acacataggg gcgagcaatg tcatgacagt
     4561 gtaggtgcgg ttactgtcgt gaaaacaata aaaaccgcca ttgcaacaat ggcgcaattc
     4621 ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc cgtatcttca gcttattgat
     4681 tccttttttc tttttcactt ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt
     4741 aactgtggaa gcgaagaatg aaacctttgc cccgcagcat cccgatcaat atctctcctg
     4801 gaaagccacc tcggaacagt cagagcgtgt tgacgccctg gcggaagatc cacggctggt
     4861 gatcctgtgg gcggggtatc ccttctcgcg cgattacaac aagccgcgtg gacatgcctt
     4921 tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg ccgaaaaacg ctgaagatgg
     4981 tccgctaccg atggcgtgct ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa
     5041 agacggcgaa gatggctact tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa
     5101 caacttaggt tgtgccgact gccataacac cgcctcacca gagttcgcca aaggcaaacc
     5161 ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt
     5221 tgagaaagcc ggacgtttcg accagcaatc gatggtttgc ggtcagtgcc atgtggagta
     5281 ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg gatgacggca tgaaagtcga
     5341 aaatatggag cagtattacg acaaaattgc cttctctgac tggactaact ccctgtcgaa
     5401 aacgccaatg ctgaaagcgc agcacccgga atatgaaacc tggacagcgg gcattcacgg
     5461 taaaaacaac gtgacctgta tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa
     5521 actctacacc gaccataaaa ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa
     5581 ctgccatacc caggacaaag ctgccttgca aaaagtggtc gcggaacgta agcagtcgat
     5641 taacgacctg aaaatcaagg ttgaagatca actggttcac gctcacttcg aagcgaaagc
     5701 agcgctggat gcaggcgcga cggaagccga aatgaagccg attcaggacg atatccgtca
     5761 tgcccagtgg cgttgggatc tggcgatcgc ttcccacggc attcatatgc acgcaccgga
     5821 agaaggttta cggatgctcg gtactgcgat ggataaagcg gcggatgcac gcaccaaact
     5881 ggcgcgcctg ctggcgacca aaggcatcac ccatgaaatc gagatcccgg atatctcgac
     5941 caaagagaaa gcccagcagg ccattggcct gaacatggaa caaatcaagg ccgagaagca
     6001 ggacttcatc aaaacggtga tcccgcagtg ggaagaacag gcacgtaaaa atggtctgtt
     6061 aagccaataa ccccgttccg actcgcaagg ggcggaaaac acaatggagt gaatatgagc
     6121 gtattacgtt cgttattaac tgctggggtg ctggcgtcgg gcctgttgtg gagcctgaac
     6181 ggaattaccg ctacccctgc ggcgcaggca tctggtgatc gctacgaagt tacccagcag
     6241 cgtaacccgg atgccgcctg tctggactgt cataagccag atatcgaagg tatgcatggc
     6301 aaacatgctt ccgttatcaa cccgaataac aaactgccgg tcacctgcac caactgtcac
     6361 ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg
     6421 atgtacaagg tcggagagca gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg
     6481 caaaaagcgt tctggccgca cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat
     6541 tccctgcatc cacagcaaga tacgatgcag acgttaagtg acaaaggacg gattaagatt
     6601 tgcgtcgatt gccacagcga tcagcgcacc aatccgaact ttaacccggc gtctgttccg
     6661 ttgcttaagg agcagccatg acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc
     6721 tggcagccgt cagcggcacc gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg
     6781 tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg
     6841 cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg cctgatgatt attcgtagcg
     6901 agccgcaggg cgaatttcct gatgtgaaat atcgcttctt ccgtaagtct tgccagcact
     6961 gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta
     7021 gcgggattgt cgatgttaat cctgatctct gcgtcggttg tcagtactgc atcgccgcct
     7081 gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac ggcggacaaa tgcgatttct
     7141 gccgtaagac caatttgcag gccggtaagc tgcccgcgtg cgttgaggct tgcccgacga
     7201 aggcgctgac gtttggcaat ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc
     7261 agaagcctac ttaccgctac aagctggcgc tgggaaccaa accgaagctg taccgcgtac
     7321 cgtttaaata cggggaggtg agccaatgac gcagacttcc gcatttcatt ttgaatcgct
     7381 ggtgtgggac tggccgattg ccatctacct gtttttgatt ggtatttctg ccggtctggt
     7441 gacgctggcc gtgctgttac gtcgcttcta cccgcaggcg ggcggtgcag acagcacgtt
     7501 gctgcgcacc acgctgattg tcggacctgg cgcggtgatc ctcggtctgt tgatcctcgt
     7561 cttccacctg acaagaccgt ggaccttctg gaagctgatg ttccactaca gttttacctc
     7621 ggtgatgtcg atgggggtga tgctgtttca gctctacatg gtggtgctgg tgctgtggct
     7681 ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa cgctggttgc cgaagctggg
     7741 gatcgtgcaa aaggttctga gcctgctgac gcccgttcat cgcggactgg aaacgctgat
     7801 gctggtgttg gcggtgctgc tgggggctta taccggcttt ctgctgtcgg cgctgaaatc
     7861 gtatccgttc ctcaataacc cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc
     7921 cggtgtggcg gtagcgctga tcgccatggc gatacgccaa cgcaaaaatc cgcattccac
     7981 ggaagcgcac tttgttcacc gtatggaaat ccccgtggta tggggtgaaa tcttcctgct
     8041 ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt aaagtgcgtg cgctggtggc
     8101 ggcattaggt ggcggtttct ggacgtggtg gttctggctt ggtgtcgccg ggctggggct
     8161 gattgtgcca atgttgctca aaccgtgggt caatcgcagt tccggcattc ctgccgtgtt
     8221 ggcggcgtgt ggggccagtc tggtcggcgt gttgatgctg cgctttttca ttctctacgc
     8281 cggacagttg acggtggcgt aagccagaaa agaggtggtt tctggacgta ttccttcctg
     8341 aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg
     8401 cctttgcggg agtgcggttg cgctggcctg ccatgatgcg actcacttgc atcggcattc
     8461 tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact gacgtattgt tttctcatca
     8521 gcgatttctc ggtcatttat gtcgcgcaac atagctatag cctgctgtcg tgggaactca
     8581 agctggcagc ggtgtggggc ggccatgaag gttcgctgct gctttgggtg ctgctgcttt
     8641 ccgcctggag cgcgctgttt gcctggcatt atcggcagca aaccgatccg ctatttccgc
     8701 tgacgttagc cgttttatct ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt
     8761 ccgatccctt cgtgcggata tttccaccag caatcgaagg ccgcgatctc aatccgatgc
     8821 tgcaacatcc cggtcttatc tttcatccac cgctgcttta ccttggctat ggcggtttga
     8881 tggtagcggc gagcgtggcg ctggcgagtt tactgcgcgg cgagtttgat ggtgcctgcg
     8941 ccagaatttg ttggcgctgg gcactacctg gctggagcgc attaacggcg gggatcatcc
     9001 tcggttcctg gtgggcctat tgcgaactgg gctggggcgg ctggtggttc tgggatccgg
     9061 tggaaaacgc ctctttatta ccctggcttt ctgccactgc gctgctgcac agtttgtccc
     9121 tgacacgcca gcgggagatt ttccgccact ggtcgctgtt gctggcgata gtaactctga
     9181 tgctgtcgct actgggtacc ttaattgtcc gttctggcat tctggtttcg gttcatgcgt
     9241 tcgcactgga taacgtccgc gccgtgccgt tgttcagcct gtttgcactg attagccttg
     9301 cgtctctggc tctgtatggc tggcgagcgc gggacggtgg cccggtggtg cgtttttcgg
     9361 ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct gttttgcgca gtgctactga
     9421 tcgtgctggt gggaacgctt tatccgatga tttacggtct gctgggctgg ggacgcctct
     9481 ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt tggcctgttg atgctggtgg
     9541 tgattgtgct ggcgacgttt gtctctggca aacgcgtgca gcttccggcg ctggtagctc
     9601 atgcgggcgt gctgttattt gccgctggga tcgtggtttc cagcgtcagc cgtcaggaga
     9661 tcagcctgaa tttacagccg ggtcagcagg tgacgctggc aggatacacc ttccgttttg
     9721 agcgcctcga tctgcaagcc aaaggcaatt acaccagcga aaaagcgata gtggcactgt
     9781 ttgaccatca gcaacgcatt ggtgaactga tgccggagcg gcgtttttac gaagcacgtc
     9841 gtcagcaaat gatggaaccg tcaattcgct ggaacggcat ccatgactgg tatgcggtca
     9901 tgggtgaaaa aaccggagcg gatcgttacg cttttcgctt gtatgtacaa agcggtgtgc
     9961 gctggatctg ggggggagga ttgttgatga ttgcgggcgc attattaagc ggatggcggg
    10021 ggaggaagcg cgatgaataa aggacttctc acgctactgc tgttatttac ctgttttgcg
    10081 cgcgctcagg ttgtagacac ctggcaattc gccaatccgc aacaacagca acaggcgtta
    10141 aatattgcca gccagttacg ttgtccgcag tgccagaatc agaacttact ggaatccaat
    10201 gcaccggtgg cggtcagtat gcgccatcag gtttacagca tggtggcgga ggggaagaac
    10261 gaagtcgaaa tcattggctg gatgaccgaa cgctacggag attttgttcg ctataaccca
    10321 ccgttaacgg gtcagacgct ggtgttatgg gcgctgccag tggtgttgtt actgctgatg
    10381 gcactgatcc tctggcgagt gagggcgaag cggtgaaaca gtcacaaata ccggtgaaaa
    10441 tgctgacaac actcacgatt ttgatggtat ttctctgtgt cggcagttat ctgttaagcc
    10501 caaaatggca ggcggtacgt gcgcaatatc agcgtcagcg cgatccgcta catcagtttg
    10561 ccagccagca aaccccggaa gcgcagcttc aggtattgca ggataaaatc cgcgctaatc
    10621 cacaaaacag cgaacagtgg gcgttactgg gcgagtacta tctgtggcaa aacgattaca
    10681 gtaattcgct gctggcgtat cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt
    10741 atgcggcgct ggcgacggtg ctttattacc aggccagcca gcatatgacc gcccagactc
    10801 gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga aatcaccgct ctgatgctgc
    10861 tggcttccga tgcgtttatg caggcgaatt acgcgcaagc catcgaacta tggcaaaaag
    10921 tgatggatct caactcaccc cggatcaacc ggacacagct ggtcgagtcg attaatatgg
    10981 cgaaattgtt gcagcggaga tcggattaat catctggtga tgaagattat gttattgagc
    11041 tgcataaaaa ataatcgaat gaacatatgc caaaaataat caccaatcag tattattgca
    11101 gattaaacaa ataaaaatct ttccataaca aatggttatt cattaatcct gccataaact
    11161 cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc
    11221 gcgcaaatga tactgatcat aagcgttaaa aaaatctaca aaccaacgca acacaattca
    11281 tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg gaacggcgct ccattgagga
    11341 agttattctg atgaaaaata taaaattcag cctggcctgg cagattctgt ttgctatggt
    11401 gctgggcatt ctcctgggaa gctacctgca ctaccatagc gacagccgcg actggctggt
    11461 cgtcaatttg ctctctccgg cgggtgatat cttcatccat ctgattaaaa tgatagttgt
    11521 gccgattgtg atctccacgc tggtggtggg tatcgcgggt gttggtgatg ccaaacagct
    11581 cgggcgtatt ggcgcgaaaa ccattatcta cttcgaggtg atcaccacca tcgctatcat
    11641 tttggggatc actctggcga acgtcttcca gcccggtgcc ggggtggata tgtcgcagct
    11701 ggcgaccgtc gatatctcga aatatcagag cactacggaa gcggtacaaa gcagttccca
    11761 cggcattatg ggcacgattt tgtcgctggt gccgacgaac attgtggcgt cgatggcgaa
    11821 aggcgaaatg ctgccgatca tcttcttctc ggtgctgttt ggtctggggc tttcctccct
    11881 gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc tccatctctg aaaccatgtt
    11941 taaagtgact cacatggtga tgcgttatgc accggtgggt gtgtttgcgc tgattgcggt
    12001 gacggtggct aactttggtt tctcttctct gtggccactg gcgaaactgg tgctgctggt
    12061 gcatttcgcc attctgttct tcgcgctggt agtgctggga attgtggcgc gcctgtgcgg
    12121 gttaagcgtc tggatcctga ttcgtattct gaaagatgag ctgattctgg cgtactccac
    12181 tgccagctct gaaagcgtac tgccgcgaat tattgagaag atggaagcct acggagcacc
    12241 ggcgtcgatc accagcttcg tggtgccgac cggttactct tttaaccttg atggttcgac
    12301 gctgtatcaa agtattgccg ctattttcat cgctcagctg tatggcattg acctgtccat
    12361 ctggcaggaa atcattctgg tgctgacgct gatggtgacc tcgaaaggga ttgctggcgt
    12421 gccgggcgtg tcgtttgtgg tgttgctggc aacgctgggt agcgtaggta ttccgctgga
    12481 aggcctggcg tttattgctg gtgttgaccg tatcctcgac atggcgcgta ctgcgctgaa
    12541 cgtggtgggt aatgcgctgg cggtgctggt gattgccaag tgggaacaca aatttgaccg
    12601 caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa tttgataaaa ctgcggatca
    12661 ataattgaag attgccgggg ttatccaccc ggcaatgtgt ggatgcctga tgcgacgctg
    12721 gtgcgtctta tcaggcctac cttaaccgtt ggttaatttt tcaaactctt cacacccggt
    12781 atcaaaccct tccatacagc tcaggttcaa ccagtgcagc gccttttgtt tgttcttctc
    12841 gataaaacct tcctcaccgt ttaagaacat cattcccgcc cagtactcgg aataaccggt
    12901 tcgggaaatt gcagagctgc gtttgaaata ccaggttgcc ttgtcatcgt cggccttaat
    12961 gcccacgccg ttggcgtaaa tcaaaccaag cagcatttgg gcatcgacgg cagagtcgtt
    13021 ctccagatct tccgaggcgt tttctaacag cgaaatcgcc tttggataat ccggtttacc
    13081 cgcttgagta tttaccagaa tatgcgccag cgttacttca ccttctttac tgcccgcttg
    13141 cgccgctttc tctgcaagaa cttttgcttg tggatagtcc agactgaccg gattggtgat
    13201 tttgatctgc gccagcagcg cgcaggcatc agcatcaccg ctgtcggcgg ctttctgtgc
    13261 ccaatattcg gctttgctca aatcgccgga gctaaaccag ctgtcggcaa gaaaatattg
    13321 tgcgcgtcgg tccccggcct ctgctgcctt taaatactgg ctgccaggct cggagtcgtt
    13381 cgcgtgggca aagaatgtca aaaacaacat caatgcgata atttttttca ttttgataat
    13441 acgattgggt agggagtaac cagtataaag agttcgaatg gataaaaaaa cagcctccga
    13501 aaggaggctg tagaaaggac tgtattacgc cagtgccgct tcgcgcaggc gagttttcaa
    13561 cttgttgtac tcgtcaatca cgtactgctc ggcggcgcgc tgatcggcga tcggctcgac
    13621 gcgcacggcg cagtatttgt attccggcgt tttcgtaatc gggcttaagt tttcggtaac
    13681 cagctcgtta caggcaccaa tccaccactg gtaggtcatg taaatcgccc ctttgttcgg
    13741 acgatcgctg acctgcgcac gggtgataat tttgccttta cgcgagtgca cccaaaccaa
    13801 tgcctcatct tcaataccca gacgtttggc gtcttcggta ttgatttgtg cgtagccagg
    13861 ttcatcagcc agcgccgcca gtgccgcaca gttaccggtc atcgaacggc aagagtagtg
    13921 accaacttca cgcaccgttg acagtaccat cgggtactcg tcggtgagtt tgtcgattgg
    13981 cgctacccag tcgcaggtga agaactgcgc cagaccgttc ggggtatcaa acttctcttt
    14041 aaacagataa gaagtcccct gatcggcatc tgaagtatcg cggcaaggcc actgaatgaa
    14101 gcccagttcg cccattttct cgtaagtcgc accgtagaaa tccgggcaca gatgacgcaa
    14161 ctcatcccag atctcctggg tgttgttgta gtgcatcgga taacccatac gggtggcgat
    14221 ttcactgatg atttgccagt ccgttttcag atcccatttc ggttcaactg ctttgaagaa
    14281 acgctggaag ccacggtcag ccgcagtaaa cacgccttca tgctcgcccc acgacgttga
    14341 cggtaaaata acatccgccg ccgacgcggt tttggtcata aagatgtcct gaacgataac
    14401 cagttccaga tcttcaaagg ctttacgtac tgccgacagc tccgcgtcag tttgcagcgg
    14461 atcttcgccc ataatgtacg cggcacgcac ttcgccatgc gctgcgcggt gcggcagctc
    14521 gctgatgcga taaccggtat gagcaggcag gctttccacg ccccaggctt tggcgaattt
    14581 ctcgcggtta gccggatctt tcacgtactg gtatcccgga tacgtatccg gcagcgcgcc
    14641 catatcgcag gcaccctgca cgttgttctg accacgaacc gggtttacac ccgcatgcgg
    14701 cttaccgagg ttaccggtca gcatcgcgag gctggtcaga gaacgcacgg tttccacgcc
    14761 ctggtagaac tgggttacac ccatgcccca caggatggcg gcgcttttcg cctgggcata
    14821 catccgtgcc gcctgacgaa tctcactggc gctgacgccg gtgatatctt caaccgactc
    14881 cggcgtgtag ccttcaacga ttttacgata ctcttcaaag ccttctgtac gggaagcgac
    14941 gaacgctttg tcgtacagat tttcttcaat aatgacatgg cccatcgcat tcaacagcgc
    15001 gatgttcgag ccgtttttca gtgcaatgtg catgtcagca atgcgcgcgg tttcaatttt
    15061 gcgcggatcg cagacgataa ttttcgcccc gttacgttta gcgttaatta cgtgattcgc
    15121 cacgattggg tgggaatccg ccgggttgta cccgaaaacg aacactaaat cggtattatc
    15181 aatttcgtta atagcattgc tcattgcgcc attaccgacc gattggtgca gacctgcaac
    15241 cgatgggccg tgtcagacac gagcgcagca gtcaacgtta ttggtaccaa taacggcgcg
    15301 cgcaaatttt tgcattacat agttggtttc gttacccgta ccacgcgagg agccggtcgt
    15361 ctggatggca tccggaccgt acttctcttt gatggcgctc aggcgctcgg caacgtaatt
    15421 cagtgcctca tcccaggaaa caggttcgag tttgccgcca cgctggcgac ggatcatggg
    15481 ggttttcagg cgcggggtca ggatctgggt atcgttaatg aaatcccagc cataataacc
    15541 cttcagacac agggtaccct ggttggtttt cccctgcgct gcctccgccc ggacgatttt
    15601 gccgttatcg acgaccaggt tgattttgca acctgatgcg caataggggc aaaccgtgac
    15661 gacttttttc atcggtctcg ctccagttaa tcaaatcacg catacgcgct ctcgactaca
    15721 gtatgcatct tttatgccac attttatttg gggtcattcc ctgatattac gggcactatt
    15781 tattcaaaac tctgacgaaa aacaggctgt cgtcagtttt gacgtgacga aacgaaatac
    15841 cgcgtgacag ccatcacgcg gcagacattt tattttttct cgacgacggg agccgcctga
    15901 tacccgccgc ccagcgattt catcaactga atgctttgga tcacccggcg gctgtccagc
    15961 atcagtaatg acatctcttc ggcaagtact ggcaaccggg cttcagtggc ctgtaagcgg
    16021 ctggttaagc cgcgctgata ggcggcctcg gcagcgcgct gggtgaagcg cgttgcttcc
    16081 acgcgttcag cctgcatttc tcgctcgtcg ttgagcgttt gcagacgcgt gccgttgacg
    16141 gcaacgtcac gcaccgcgtt cagtactgac tggttgtaac gttcaatcat catgttgctg
    16201 gcggcgcgcg tgccttcgag attggcattc aaccgtccac cgtcaaacag cggcaatttc
    16261 agacccggga tgaagttgaa ctggcgactg gtttttttga ataaggtatc caggtggatg
    16321 gcatcaaggc cgaaaaacgc tttgatatca aagctcgggt agaacagcgc ccgcgcggaa
    16381 tccacctgat ctaatgacgc ctgaacatac cagcgcatgg cttgcagatc cgggcgtctg
    16441 gcgagcaact cataagagag tgtcgccgga atgccggtct ggactcgtgg taatgccacc
    16501 ggtttgatct ccggcatatc gctggccccc gcgccaatca atgcacgcag agattctcgc
    16561 gtttcggtga tttgcccttt gacggcagca atttgtttat cgaccgccag aatctgtgcc
    16621 cgcgcgccgt ggaaaggcac ttgtgcttcc agaccgtgcg ccactttact ctggtgcgct
    16681 ttcaccgcgt aatcaatcac atcgcgagtt tgttctaaca gatcgagcat ctgatagctg
    16741 gcctgcatac tgtaataaag ctgcgctacg cccgtggtca gcgatagctc tactgctgcg
    16801 gtttctgcca gcgcggcatt atgcgcgcca atggcggcgg caaccgctga gcgatgcaca
    16861 ccccacaaat caagatccag tccggcaaac aaacctactg tggcttccgt atagtacggc
    16921 ccgtccatac ccagtgcggg cgcatccatc gcataagggc ttaaaaagcc gttcgccgag
    16981 acgcgttggc ggttgagcat ccctaacgct gcgacctgta attgtgaacc ggcatctaac
    17041 aaatcggcct gggactgcgc tttttcttcc cgcagtttcg cttcggcgag ggtgtgtgaa
    17101 ccacttagcg tccgttggat cagcgcatcc agctgcgggt cattgagttg tttccaccac
    17161 tgcgcctgcg gccagccgga gctggcaaga tgaatatcgt cggccagttt gatttgttcc
    17221 ggtttgagct gttgatgagg tgcagaatcc ttacgtacca gggcacagcc ggaaatcagc
    17281 gtcgtgctgc cgagaatgct gcacaacagc agacgtgaaa gttgacgatt gatcattgcg
    17341 tggctccttg tgcctgttcc gtggcgggcg ttaatgccgg ggctgtccag tctggcaagc
    17401 gggcgaaaag ctggcagacg tgttgctcct gttttaataa ggtgggcagg atggcctgcg
    17461 aggcgggtgt ctcttcaagc gttatttgag gagaacggct gagtgcggtt gccagaccgg
    17521 cagcgtattt ctccagggcg tcggcaaact gagctgctct gtccggctgt agtgcgttat
    17581 ccagcgcctg cgccgagttc caggtggcat cccaggcgtg aagaatatcg cggccctgac
    17641 gaataaccgt ttgcgaacgt tcaatcagta aggcgcgttc ttcgctgtcc agttgacgct
    17701 ccagcgccac gcgttggcac atctcttcac aggcattaaa cgccgcatgc agaccgatac
    17761 gaatttgcag ataagtgcgc agagccgtga cttcctgctg gcgtgggatc cgcattactt
    17821 tacttaacat acccagcgtg ccagccagtt tttgcggcag tgtgcgcgct tcactttcag
    17881 gccagacaaa ggtgtaaatc accgcggaca ccacggtacc aatgatgata cccatggcgc
    17941 gatcgcgaac ttccaccagg tcgtacaccg ggccaaaaac gttttcgagc gtggcgagcg
    18001 cgaaggtgac caccatctgt gtgccgatat aagaagagcg ttcagagctg gtggcaatcc
    18061 atgcgcccaa caggaaaatc ggtgccagca caaacagcaa ttcgacaata ttgtccagcc
    18121 agggcatgac cagtagcgtg aataacagcg ccagaatcgc gccgcaaaag gccccgccaa
    18181 aacgcagcac catcttctgg tacgacgaac cgacatttgg gttagcgacg atcacgcatg
    18241 tcagcataca ggtgtgaatg ccttcccagt ccacgccgct gtagaaggtg taacagatca
    18301 aacaggcgag cagtgttttt accgcgtagc gcatatagtc tggattggta aaagcatcgg
    18361 cgaccattga tggcggtttg gcggcgggcg ttggcggcgt attcgggtcc atctgaccca
    18421 gttgtaacaa cgtctggcag atattctcca ggttacattc ccgtgccgcc atcgcttcac
    18481 tttcagtgat ccgccagtcg ctttgccagc actgaccttc agcaacggca tgctgcagct
    18541 tattgatttc tgaggctaat ttttgccgaa attcaataat tgcctgagaa tcagcaaaag
    18601 aggtgggatc gtagcgattc agcgtcgagt aaatgtaggt taccgttgcc acgcagcttt
    18661 gccaccatgc gctttgagtt cgccagttgg catcgtccgc gagacaaaag acattcagtt
    18721 tttgcagcgc cagcgcctct ctttcaatcc gcgtttcggg tagcggtgcg aggctgtccg
    18781 tcaggtgact aatggcatca tcaagccgat cattaagcgc ttgatgcatt tgcgtaatgg
    18841 cacgactggg aaaccacagc acgccgatta acgtcatcag caaggttgga tagaggccaa
    18901 caacgataca ccacagcgtt aagcgcacga ccacttccgg atagtcgagc atggcgggga
    18961 aggtttgccc gtaaatggcg acaatggcga cagcgaaaaa gaccagtccc agccgatggg
    19021 tgcgcatcaa aaacatacag cccatcagga tcggcccggc gatgatcaat cggatcaacg
    19081 gttcgccgta tgaccatttg tagatcaaaa acaggctgcc gatctccagc accgtggcaa
    19141 ccacaaacaa gatcgcgaca aatttggtgt aaaacgcgtt cgactgaata ccgtaaaaca
    19201 gcactgccag cgataacgcc acaaaaggga tctcaaaagt catcgagatc agaatcacca
    19261 gcaggcagcc tacccagagt tgcacgatct gcggcacgcg gccgggtcgc cgctcgctta
    19321 actcttcatg aaagaacgcc agcagcctga ccaccggtaa tggcagggag ttgagcgcgc
    19381 tcattattgc ggctcaagat tagcgactgc cgaagcgccg atgcggaaca tttccgggtc
    19441 aggtttatcg accatgattt tgaccggaaa acgctgggca acgcggaccc agttaataga
    19501 gcgagaaact ttcggcaggc cgcccagcac caggccgccg tcatccggta gcacgccgta
    19561 gccaatcgaa tccactttac cctcgaaggt tttgccgctg tcactcatca ggcgaatcgt
    19621 tgcgggtgta cctgagcgaa tatttttcag atcggtttcg cggaagttgg cgatcacgta
    19681 ccagtgacga gtgtcgatta gggtaaaaat agggcgcatg gcagaagcaa attgcccgac
    19741 ggaggttttg agggaaatga cccggccatc aaacggcgcg cgaacggtcg ccatttccag
    19801 atgaagtttg gtcagggcaa tatccgcttc gaccgccgca cgttgcgcca ccagcgcatc
    19861 gacgccgctg acggcgctgg cggctgactg cgcctgtaac aatacggcat taagatcggc
    19921 ttctgcggcg cgctgcgcag ttcttgcacg gtcaacgtct tccgctgaga caaaaccctc
    19981 tttcagtaat ggctcggtgc ggcgtaatgt atctgtggcc tgtttcgcgg cggcacgggc
    20041 tttttctacc gtggcattaa tcgagtcggc accaaactgt tgcgcatcaa cgctacgctg
    20101 ggtgagcata atttgcttat ccagcgccgc gagggaggct tcagctttcg ccagattggc
    20161 ttcgtacggg cgcgggtcaa tgcggaacag caaatcgccc tgtttgactg cctggttgtc
    20221 ggtgaccgcc agttctacaa tgcggccgct gacttccggc accacatcaa tggtatctgc
    20281 tgacgcgtaa gcgtcattag ttgatggcgc actgtctacg cgccagataa cgaaaacaag
    20341 ggcaaccagc gccaatgcaa ccactaacag agcagggaat ttactgcgag gagctttttt
    20401 cggcgtactt tccatgagcg cgtctcaaat agattagaaa aatgccagcc agaacagcat
    20461 ggcgtagaga gcaaaaaggg cggtataaat aattccgaca aatgccagat tgatatttgc
    20521 ccgctgaata actcgcctcg tgatgagcat caaaataagg ctggcaatgg cgcagaaaaa
    20581 ccagctggga taataagcac cgatcaccgg gattgccgga gataatgagc atcccgaaac
    20641 catgaagacg ggaattatcc aggctgtttt tttgagttgc atcgccatgc gagagagaac
    20701 tgttggcata ttaaatacat cctgtctgct gcgaacaata tctgtagtga atatcaatat
    20761 ccattgaagg cgtcagtatg gctattttct cgatgaataa aaatagcttt attcagcca
//
LOCUS       DAZFXT010000080        20577 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.080, whole genome shotgun sequence.
ACCESSION   DAZFXT010000080 DAZFXT010000000
VERSION     DAZFXT010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.080"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1256)
                     /locus_tag="ACHKW0_003169"
     CDS             complement(<1..1256)
                     /locus_tag="ACHKW0_003169"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004029 - aldehyde dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0008774 - acetaldehyde dehydrogenase
                     (acetylating) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HFO7475957.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPSAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVK"
     gene            complement(1268..1555)
                     /gene="eutN"
                     /locus_tag="ACHKW0_003170"
     CDS             complement(1268..1555)
                     /gene="eutN"
                     /locus_tag="ACHKW0_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HFO7475958.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(1662..1955)
                     /gene="eutM"
                     /locus_tag="ACHKW0_003171"
     CDS             complement(1662..1955)
                     /gene="eutM"
                     /locus_tag="ACHKW0_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HFO7475959.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(1994..3010)
                     /gene="pta"
                     /locus_tag="ACHKW0_003172"
     CDS             complement(1994..3010)
                     /gene="pta"
                     /locus_tag="ACHKW0_003172"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008959 - phosphate acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HFO7475960.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     PLGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(3007..3810)
                     /gene="eutT"
                     /locus_tag="ACHKW0_003173"
     CDS             complement(3007..3810)
                     /gene="eutT"
                     /locus_tag="ACHKW0_003173"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006580 - ethanolamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HFO7475961.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(3807..4508)
                     /gene="eutQ"
                     /locus_tag="ACHKW0_003174"
     CDS             complement(3807..4508)
                     /gene="eutQ"
                     /locus_tag="ACHKW0_003174"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HFO7475962.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(4483..4962)
                     /gene="eutP"
                     /locus_tag="ACHKW0_003175"
     CDS             complement(4483..4962)
                     /gene="eutP"
                     /locus_tag="ACHKW0_003175"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HFO7475963.1"
                     /translation="MKRIAFVGTVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLMDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(4975..5310)
                     /gene="eutS"
                     /locus_tag="ACHKW0_003176"
     CDS             complement(4975..5310)
                     /gene="eutS"
                     /locus_tag="ACHKW0_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HFO7475964.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(5603..7882)
                     /gene="maeB"
                     /locus_tag="ACHKW0_003177"
     CDS             complement(5603..7882)
                     /gene="maeB"
                     /locus_tag="ACHKW0_003177"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HFO7475965.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            8171..9121
                     /gene="tal"
                     /locus_tag="ACHKW0_003178"
     CDS             8171..9121
                     /gene="tal"
                     /locus_tag="ACHKW0_003178"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7475966.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            9141..11144
                     /gene="tkt"
                     /locus_tag="ACHKW0_003179"
     CDS             9141..11144
                     /gene="tkt"
                     /locus_tag="ACHKW0_003179"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HFO7475967.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(11240..12283)
                     /gene="ypfG"
                     /locus_tag="ACHKW0_003180"
     CDS             complement(11240..12283)
                     /gene="ypfG"
                     /locus_tag="ACHKW0_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HFO7475968.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(12409..12984)
                     /gene="nudK"
                     /locus_tag="ACHKW0_003181"
     CDS             complement(12409..12984)
                     /gene="nudK"
                     /locus_tag="ACHKW0_003181"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HFO7475969.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(13052..15031)
                     /gene="aegA"
                     /locus_tag="ACHKW0_003182"
     CDS             complement(13052..15031)
                     /gene="aegA"
                     /locus_tag="ACHKW0_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HFO7475970.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            15237..16937
                     /gene="narQ"
                     /locus_tag="ACHKW0_003183"
     CDS             15237..16937
                     /gene="narQ"
                     /locus_tag="ACHKW0_003183"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HFO7475971.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            17101..20214
                     /gene="acrD"
                     /locus_tag="ACHKW0_003184"
     CDS             17101..20214
                     /gene="acrD"
                     /locus_tag="ACHKW0_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HFO7475972.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(20313..20372)
                     /gene="ypfM"
                     /locus_tag="ACHKW0_003185"
     CDS             complement(20313..20372)
                     /gene="ypfM"
                     /locus_tag="ACHKW0_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HFO7475973.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT         4706 a         5600 c         5360 g         4911 t
ORIGIN      
        1 ttcttaacga aaatgctggt atcaatagca ttcgccatct ggttcatgtt ttcgatgttg
       61 cgcgagtgca ttgccgccgt gtggtggcaa ccgccttcca gtttcaccgc cagcgcaatg
      121 gcatccgcca cgttggcgac gcgcacgacg ggcagcaccg gcatcatcag ttcggtcacg
      181 gcaaacggat gttctgcagt ggtttccaca aacagcaaac gcgtttcttg cggcacgttc
      241 aggccgattg ccgccgcgat tttcgccgca tcgcgaccaa cccagtcacg gctgacggtg
      301 ccttttccgc gctcgtcgat atttttcagc aacaccggtt gcagctgctg cgcctgttct
      361 gcggtcagtt tcaccgcgtg ctggccttcc atcagacgca tcagttcatc ggctacgcta
      421 tcaacaacaa tcagtacctt ttcgtcggca caaatgatgt tgttatcgaa agacgcgcct
      481 ttgacgatgg actgagcagc acgggcgagg tcagcggttt catccaccac taccggcggg
      541 ttgccagcgc ctgcggcaat cagacgttta ttggtgtgtt tacgcgccgc ttctactacc
      601 gcttcgccgc cggttaccac cagcaggccg atacccggaa acttgaacaa gcgttgcgcg
      661 gtttcgatat ccggatttgc cacagtaacc agtaagtttt ccggcccacc tgcggcaaca
      721 atcgcctggt tgagcagcgt aatcgcccgc tgggagactt ttttcgccgc cggatgcggg
      781 gcaaaaatga cgctgttgcc cgcggcaatc aggctgatgg cgttattaat tacggttgcc
      841 gccgggttag tggacggagt taccgaagcc accacgcccc agggcgcgtt ttcaattaaa
      901 gtcaggccgt tgtcgccggt cagcacctgc ggagagagac actcaacgcc tggcgtgccg
      961 cgcgcctgag cgacgttttt ggcaaattta tcttcaacgc ggcccatgcc ggtttcactg
     1021 acggcaagtt ccgctaaatc tctggcgtgt ttttcgcctg cctcacgaat ggcagcaatg
     1081 gctaactggc gcattgccac gctttttaac ccttgctggg cgactttggc tgccgcaacg
     1141 gcgtcatcca gggacgcgaa aacgcccatc tcatgaacgg cggcggacgg cgtgtcactg
     1201 ctttgcattt tcagcagtac cgctttcacc acctgttcaa tatcctgttg attcatgatg
     1261 ttctgcctta tttgtggaaa attacctgac cgccagacac cacctcatcg acaatgccaa
     1321 tcacgcacag atcgaccggt gacgtttcgc ttttatgcgc ctggcgggcg gaactgccac
     1381 tcaccagtaa cacccactcc ccggttcccg cgccaatatt gtcgatggcg acggcgcatt
     1441 gcccgtcagg attaccttgt ggatcaatca tttccaccat cagcaatttg tcatgcgcca
     1501 gtccgtgatg gcgtacggta caaacaattt gtccagtgac gactgccagt ttcatacccg
     1561 cctccgtggc gtatttcagg taaaagctcc ccctaccctc cgcagaaggt aaaatgaaaa
     1621 aggagagagc gtgacgcccg aatcgacgtc acacagggtg attacaggtt gctgctatcg
     1681 cctttcaggc cgatcgggaa gacttcttcc aggtcgccgt gcggacgcgg gataacgtgt
     1741 acagatacca gctcgccgat acgctgtgcc gctgcggcac ctgcatcggt cgcggcttta
     1801 caggctgcaa catcaccacg aaccatggca gtacacaggc cgccgccaat ctgcttaaca
     1861 ccaaccagct tgacgcgcgc agctttcacc atcgcgtcag aagcctcaat cagtgcaacc
     1921 aggccccggg tttcgatcat tcctaatgct tccattgtgc tttcctcttt atcagggtcc
     1981 agaacgggac cgttcattca accagtgttt gtaaactgct ttcgcggttc acttctgtct
     2041 gacgcggcac agctgccacc agcgccagct cgataatttc ctgcacgcta caaccacgag
     2101 agagatcgtg catcggcgcg gcaagtcctt gtatcagtgg cccgacggca cgatatccgc
     2161 cgagtcgttg tgcgattttg taaccaatat ttccggcttc cagcgacgga aaaaccatca
     2221 cattggcctt gccctgtagc gggctggcag gcgctttttg cgccgccact tccggcacga
     2281 aggcggcgtc aaactgtaac tcgccatcca ccaccagctt tggtgcgcgc tcacggacga
     2341 tttctgtcgc ctgctggacg ttagcaacac aggggtgacg ggcgctacca ttgctggaaa
     2401 acgacagcat cgccacgcgc ggctcttctc cggtgatggc gcgccaggtt tcggcactgg
     2461 caagcgcgat atccgccagc tgcgccgccg tcggctgtgg caccacgctg caatcggcaa
     2521 agcccaacgg cgggccgctg tactgtggca acatcaggaa aatggaagag agcgttttac
     2581 agcctggctg caagccgata atgcgtaatc cggcacgcag cacatttgcc gtggaagaga
     2641 ggttgcccgc gatacagaca tccgctttac cggcgctgac cattgctgcg gcgaacatca
     2701 gagggtcggt aagtttttcc agcgcatccg gcggcgtttt ttcgcccgcg cgggccagcc
     2761 agcgatgagc aaattcttcc cgcattgcga ggttgccatg cggatcaatc acctgtagcc
     2821 cgtccatcgc cacgccgtga ctgagcgcaa actgacgaag ttcaaacgga ttggcgacca
     2881 gaatgggcgt tgccagacct tgttgatgta aatattgcgc ggctttcagc acacgttggt
     2941 ctaacgcatc cggaaaaacc actctggcgg gcgctcgcaa cgccagttca cgacaacgtt
     3001 caataatcat tgcgtttccc ccagtcgttg ttggatctgc ttcaccgtta gcggctgctt
     3061 cgttacgctc aggatcatca tcacgtagac cgtgctggaa aggcggttca gcgcctgcaa
     3121 aatgtccggg cgtaatactt caaagctgcg ggtgataaac acctgcgccg ctacggtttc
     3181 ggtttcccgc actttggtac gtagcagatt gagcaacgcc gcatcgcgcc cgtggctggc
     3241 ttccggcacc agatgatcgt ggtcgagata gcgcagcggc tggtgagaaa gtcgatgcag
     3301 atcttcgtca gaaagcccga caatcgcctg gcagcccagc ggttcaccca gcgcatcggc
     3361 gcgcataatg ttgccaagac gcgaacggat atccgccagc cacggctgcc acggttccgc
     3421 cagttcaatt tgcagccaca ctgccagcgc aatggtgctg tcgagaaccg cgcgaaaacc
     3481 cagacgcgga tcgcttttgg cgaccatttt ttccgccgac aggtgagtca gcgtatccgg
     3541 cttttttgcc accggctggc ggcacagttc gcagcacgcc tgcggatgtt catcgctact
     3601 ggtcagccca tgaacgggct gcggctgctg ctgttcatcg tcaacaaaca ggcggccctg
     3661 ctcgtcaata aacttgatgc gcagatggcg gctttccagt aactcccggg cagagggcgt
     3721 caggcgactg tccgcaggga gatggatctc tgcgccttcg ctgagcgtat ggttcgctct
     3781 tagccatgct tcggtgatga aatctttcat agggattgcc agttagccgg ccaggcgaca
     3841 tacaggaatt tcacgctgga tgtcgtacca aattcgatac tggagccttt cgggataaac
     3901 atcacgtcac ccgctttggc gatcatggtt tcgccttcgt ggcgaacatg cagctcccct
     3961 tccagcacca tgtcgatttc gtcgtagttc agggtccacg ggaagaatgc gttctcccac
     4021 tgcataaaac cagcggccat gctgctgccg tcgtctccgg tcacaagatc agtcaagccc
     4081 acgcaatgcg gctctgcgcc gtcgaaacga ccaaacttga cgctgctgcc atcaatcact
     4141 ttgatgccgc ctttgccggt caccgatttg aagctcggct gcattgcgcc ctgctccagc
     4201 gactgttttt ccttcatcac tttttccatc agctgtgcga ccaggctttc ggtaaactgg
     4261 ccttccggca gctgggcaat gatggtttcg cggatgcgct ggctttcggt tttgtcggct
     4321 ggcacgctgg cgggcacgga cgccgttacc gggatcgatt catcacattc ggtgatggta
     4381 aaacccagta gatccgccac ttcacgggct tccggggtaa taatgctggc gcgcagaacc
     4441 acggacattg cctgttcgcc gcgcgcgtgt gcttcacgaa tatcattcgc tgtgatgagt
     4501 tttttcacct gcttcctcct gtttggtgag agatgccaga taatccacca gttgctgcac
     4561 gctttgcggg tcgtggctgt ttagctcgaa aatcggctct tcgaaacccg tctcaagcaa
     4621 taactttcgc gttgcggcga cgtcagcatc tggcatgtcc gttttactga tgacggcgat
     4681 ttgccgtttg ctgacgccaa tatccatcaa cccggcaggc aaccgacttt ccgggtcatt
     4741 tgcgccatgg acatagatca gcatgtctac atcctgtagc gtggtaatta aggcgtgata
     4801 ccagcgggga tggctaaaat attcacccgg cgtatcaata tcgcccttat cattaaattc
     4861 cacggcctgt gtttttctgg cgagggtata attcccctgc aacgcattaa ataacgttgt
     4921 ttttcctgcc ccgacggtac ccacaaacgc aattcgtttc atgaatgcct cgtattaact
     4981 tttggtcatt tcacaaagcg tataatttaa taagcgacct aaaccgctga cggtttgtga
     5041 taacgcttct tctactgcgc ccaccgagcc ataaatcacc aatgcaccgc tgaagcggtc
     5101 gagaaaaccg atatgcacat cagcggcttt cagcgctaaa tcaccggcga tcatcgccgt
     5161 ttcgccggga gttagcgtca taatgccaat tgcgcccgca tccggaacgc cgattttctt
     5221 cgccagttcc tcaccgggat gcgcgatgag atgcgccagt gtgacctgtt tgccgggcac
     5281 aaattcctga atgatgcgtt ctttatccat gagtcgccgc ctgtgaaaaa ctttcgtgta
     5341 tcgtgacaat ttttcgtcag agtaaataac aaaattcggc aagcgcgttt aaaaggtgag
     5401 gtagatcact aagaatggag agatattcgc tgtggtgcat aaactttcat atgcaacgca
     5461 tgaatataaa taaaataata tcgagatatt atatagactg ctttttttat tattcgcaaa
     5521 ctcagtaata agctttacta atatattgca gccaaaccgc ttaccctgaa tattcagggt
     5581 aagcgtgaga gttaaaaaag aattacagcg gttgggtttg cgcttcaacc acggccagcg
     5641 ccaccatgtt gacgatacga cgcaccgatg cgatcggcgt taacacgtga accggtttcg
     5701 ccacgcccat cagcaccgga ccgacagtca caccttccga gctggaaacg cgcagtaagt
     5761 tgtaactaat gcgggcagct tccatgttcg gcatcaccag aatattggca gaacctttca
     5821 aagggctgtc cggcatacgg tcgttgcgaa tcgcttccac cagcgctgca tcgccgtgca
     5881 tttcaccatc aatcatcaga tctggcgcac gatccctgac cagttccagc gcctgacgca
     5941 ttttgctcga cgacgggcag tcagaagaac caaagttgga gtgcgacaac agagcaacgc
     6001 gcggctcaat accaaaacga cggacagttt ctgccgccat caaggtgatc tccgccagct
     6061 cttctgcgtc tggttcgtca ttgacgtagg tatcggcaat aaaggtgtta ccactcggca
     6121 gcagcagcgc gttcattgca cctgcggtgt gaacgccatc gcgataacca aagacatttt
     6181 tcaccacgct aaaatgttca tgataatcgc ccaccgtacc gcagatcatt gcatcggctt
     6241 ccccacgctg aaccatgatc gcgccgatca ctgtcgggtt actgatcagc gcccgctgcg
     6301 cctgttcctg agtgacaccg cgacgcttca tgatctggaa gtattcggtc cagtactctt
     6361 taaagcgcgg atcggattcg ttattgacga tctcaaaatc aacgcccgct ttgatctgca
     6421 agcccagttt ctgaatgcgc atttcgatca cgttcggacg accgataagg atcggtttcg
     6481 ccagtcccag cgttaccagt tcctgagtgg catgcagaac gcgcgcctct tccccttctg
     6541 gcagaacaac gcgcttcggc gctttgcgag cctgggagaa aatcggcttc ataaacaggt
     6601 tggttttgta aacgaactca gtcagcttgt cgatgtagac gtcgaaatca gcaatcggac
     6661 gcgtcgccac gcccgactcc atcgcggctt tagcgaccgc aggagcgatc ttaacgatca
     6721 agcgcggatc aaacggtttt ggaatgatgt attccggacc aaagctcaga tcctgatcgc
     6781 catacgctga agccaccact tcgctctgtt cggcgtgggc cagttccgca atcgcgcgta
     6841 ccgccgccag tttcatctct tcattgatag ccgttgcgcc aacatccagc gcgccacgga
     6901 agataaacgg gaagcaaagg acgttgttga cctggtttgg atagtcagaa cgaccggtgc
     6961 aaatgatggc atccggacgc acttctttcg ccagcggcgg cagaatttcc ggttccgggt
     7021 tcgccagcgc caggatcatt ggcgcacgag ccattttctt caccatttcc tgggtcagca
     7081 ctttcgggcc ggaacatcca aggaaaatat ccgcgccttc aatcacatca tcgagggtac
     7141 gtttgccgtc atccaccacc gcatatgcgg ctttggtttc cgccatgttt ggctcacggc
     7201 cctgatagat aacgcctttt gaatcgcaaa ccacgatgtt atgtttttgc agacccagcg
     7261 ctaccagcag gttcatacag gcgattgctg cggcacccgc gccggaaacc accatccgca
     7321 cgtcggagat gtttttctcc accacgcgca agccgttgag gatggcggca gtgctgatga
     7381 ttgccgtgcc gtgctgatcg tcgtggaata ccggaatatt catccgctcg cgcagtttct
     7441 gttcaatata gaaacattct ggcgctttaa tgtcttcgag gttgattccg ccgaaggtcg
     7501 gttcgagcgc agcgacaact tcaataaatt tgtccgggtc gagttcatca acttcaatgt
     7561 caaatacatc aatcccggcg aatttcttaa acagaacgcc cttgccttcc atcaccggtt
     7621 tgccagccag cgcgccaatg ttgcctaacc ccagcaccgc cgtaccgtta gagatcaccg
     7681 ccaccaggtt accgcgcgcg gtatatttgt aggcttttaa cgggtctttt tcgatttcaa
     7741 gacaaggtgc ggcaacgcct ggtgagtagg ccagcgccag atcgcgctgc gttgccagag
     7801 gcttggttgg agaaacctgg attttccctg gaactggaaa ttcatggaaa tcaagtgcac
     7861 tttgttttaa ctggtcatcc atttggttgt tcctttcacg taacgttcac aaataaagtg
     7921 tgtgggcaac agcccctgcc cacaacgtgg cgcacattat taccctgccg gagtctacag
     7981 actttgagca agtccaaact ctcaccatta atataatgtt ttggtaataa tcctataaca
     8041 ctgatgttac ctgcttaatc cagcaatacc atgcctgtct gctatgcttt tttgatgcgt
     8101 ttagcgaaat ttctcagaag tgtgaattaa cgcactcatc taacacttta cttttcaagg
     8161 agtatttcct atgaacgagt tagacggcat caaacagttc accactgtcg tggcagacag
     8221 cggcgatatt gagtccattc gccattatca tccccaggat gccaccacca atccttcgct
     8281 gttactcaaa gctgccggat tatcacaata tgagcattta atagacgatg ctatcgcctg
     8341 gggtaaaaaa aatggcaaga cccaggaaca acaggtggtc gcagcgtgtg acaaactggc
     8401 agtcaatttc ggtgctgaaa ttctcaaaat cgtacccggt cgcgtgtcaa cagaagttga
     8461 tgcacgcctc tcttttgata aagaaaagag tattgagaag gcgcgccatc tggtggactt
     8521 gtatcagcaa caaggcgttg agaaatcacg cattttgatc aaactggctt cgacctggga
     8581 aggaattcgc gcggccgaag agctggaaaa agaaggtatt aactgcaacc tgacgctgct
     8641 gttttctttt gcccaggcgc gggcctgtgc agaagcgggc gtttttctga tttcgccgtt
     8701 tgtcgggcgt atttatgact ggtatcaggc acgcaagccg atggacccgt atgtggtgga
     8761 agaagatccg ggcgttaaat cggtgcgcaa tatctacgac tactataagc aacaccacta
     8821 tgaaaccatt gtgatgggcg cgagcttccg tcgcaccgaa caaatcctcg ccttaaccgg
     8881 ctgcgatcga ctgactatcg caccgaattt gctgaaggag ctgcaggaaa aagtttcgcc
     8941 agtggtacgt aaattaatcc caccttctca gacgttccca cgcccagctc ccatgagcga
     9001 agcggagttc cgttgggagc acaatcagga tgcgatggcg gtagaaaaac tgtctgaagg
     9061 cattcgtctg ttcgctgttg atcaacgcaa actggaagat cttcttgccg ccaaactata
     9121 aaccagccac ggagtgttat atgtcccgaa aagaccttgc caatgcgatt cgcgcactca
     9181 gtatggatgc ggtacaaaaa gccaattctg gtcatcccgg cgcgccgatg ggcatggctg
     9241 atattgccga agtgctgtgg aacgattttc ttaaacataa tcctaccgac ccaacctggt
     9301 atgatcgcga ccgctttatt ctttccaacg gtcacgcgtc gatgctgctc tacagtttgc
     9361 tacatctgac cggttacgac ctgccgctgg aagaactgaa gaacttccgt cagttgcatt
     9421 cgaaaacccc aggccacccg gagattggct atacgccagg tgttgaaacc accaccggtc
     9481 cgcttggaca aggtttggcg aacgccgtcg gactggcgat agcagagcgt acactggcgg
     9541 cgcagtttaa tcagccggat catgaaattg tcgatcactt cacctatgtg tttatgggcg
     9601 acggctgcct gatggaaggt atttcccacg aagtctgttc gctggcgggc acgctgggac
     9661 tgggcaagct gattggtttt tacgatcaca acggtatttc catcgacggt gaaacagaag
     9721 gctggtttac cgacgatacg gcaaaacgtt ttgaagccta tcactggcat gtgatccatg
     9781 aaatcgacgg tcacgatccg caggcggtga aggaagcgat ccttgaagcg caaagcgtga
     9841 aagataagcc gtcgctgatt atctgccgta cggtgattgg ctttggttcg ccgaataaag
     9901 caggtaagga agaggcgcac ggcgcacctc tgggggaaga agaagtggcg ctggcacggc
     9961 aaaaactggg ctggcaccat ccgccatttg agatccctaa agagatttat cacgcctggg
    10021 atgcccgcga aaaaggcgaa aaagcgcagc agagctggaa tgagaagttt gccgcctata
    10081 aaaaggctca tccgcaactg gcagaagagt ttacccgtcg gatgagcggt ggtttaccga
    10141 aggactggga gaaaacgact cagaaatata tcaatgagtt gcaggcgaat ccggcgaaaa
    10201 tcgccacccg taaggcttcg caaaatacgc ttaacgctta cggaccgatg ctaccggagc
    10261 tgctcggagg ttcagcggat ctggctccca gcaacctcac catctggaaa ggctctgtgt
    10321 cgctgaagga agatccggcg ggcaactaca ttcactacgg ggtgcgtgaa tttggcatga
    10381 ccgctatcgc caacggcatt gcgcaccacg gcggctttgt gccgtatacc gcgacgttcc
    10441 tgatgtttgt tgaatacgcc cgtaatgccg cgcggatggc ggcactgatg aaagcacggc
    10501 agattatggt ttatacccac gattctattg gcttagggga agatggtccg acgcaccagg
    10561 ccgttgagca actggccagt ctgcgcttaa cgccgaattt cagcacctgg cgaccgtgcg
    10621 atcaggtgga agcggcggtg ggctggaagc tggcggttga gcgccacaac gggccgacag
    10681 cactgatcct ctcaaggcag aatctggccc aggtggaacg tacgccggat caggttaaag
    10741 agattgctcg tggcggttat gtgctgaaag acagcggcgg taagccagat attattctga
    10801 ttgccaccgg ttcagagatg gaaatcaccc tgcaagcggc agagaaatta gcgggagaag
    10861 gtcgcaatgt acgcgtagtt tccctgccct cgaccgatat tttcgacgcc caggatgagg
    10921 aatatcggga gtcggtgttg ccttctaacg ttgcggctcg cgtggcggtg gaagcaggta
    10981 ttgccgatta ctggtacaag tatgttggtc tgaaaggggc aattgtcggg atgacgggtt
    11041 acggggaatc tgctccggcg gataagctgt tcccgttctt tggctttacc gccgagaata
    11101 ttgtggcaaa agcgcataag gtgctgggag tgaaaggtgc ctgatggtga tcgccggatg
    11161 ctgattgccg gatgcgacgc tgacgcgtct tatccggcct acagcgtctt atccggccta
    11221 catgtccccg caattttgtt taacgggtga tccacaacgt gggccaggcg tctggcccat
    11281 gccagttatc gcaggtgggt tctgcggcat aacgcaccag gcgaaaacgc tgaccgtcaa
    11341 agcgccagcg cgcctgaatg ccacaatcgc ttaatccgcg ccctttcgct aaggtcacca
    11401 gttcacgcga tttctcatca aatgttgcgt tcatcagttc cagttcattc gtctcctgac
    11461 cgttgttgaa tggcaaacgc aaccgaaccg ggcgcgaggc tagtggcttt ttacgcgaca
    11521 caatccatgc caaatcaatg gtgttatagg cccctgcctc acagctaatc atcatcagcg
    11581 ctttatcatc agtcagcgca gtgacattca cctcacgacg taacggatca agcgagcagc
    11641 gcagaccatt catccgccag tttccataat ccagcaaatc gttgcgttct tcgagtgaga
    11701 gtggcgtcgg cgttgggtta accaccgcga cctctttcag cgcaggcgcg ggcggtacgc
    11761 tgagcggcgg ttcgtcccct ttcttgatcc acgcggtttc actgccaacg cgcttttgct
    11821 gagcatcaat aaacaacaac gccgctttta agccactcag agaaatggtc tgattgccgt
    11881 cgcgtaaggt gatcgccttc ccttcctgaa tcatctgcaa aaacgcggtg atggttgccg
    11941 tatcatcggt tactaataac catggtgaaa tccgccactt gtcaccactt aacgccaacg
    12001 gctcaccatc taacagcagc cgtggcgcta tctctccttc agacgcatcc ggcgacttca
    12061 acccgccgcg ctcaatacgt aaaacggcat cggtatgcgc cccggcgctg cggctcaggg
    12121 tcatcaccag tccattatga tcgcccgtat tgcgcgccac gcagaagttt tggttattac
    12181 aggtgacctg ccagtcggaa aacgcccgtt gcgctggtgc cgcccacacc aaagacgtcg
    12241 gcaacaaagc gaaaaaaaag agaaggaaaa tgcgatagcg catggacggt acgaccccag
    12301 aagttcaaac aaacagcaca agtcgtatct tcgttgcgca ggtagcgcgg ctcaatcaga
    12361 tttgtcggat tgagccaata ataaacaacg aaatattatt gttatttttc agtccattaa
    12421 atgtgacgtt tgcaaatagt taagcaataa caccgtctta ccgtcacgta tctcgccggt
    12481 tttgatcatc tccagcgcct ggctgaacgg cagctcgagc acttcaatat cttcatcttc
    12541 aacaccgcca ccggcgttag cgcgctgatt gtcactgtat tcggcgataa aaaagtggat
    12601 tagctcagtc acaccgccag gcgacatata cagttcaaat aatttgcgca cttcgcccac
    12661 ttcatagccc gtctcttcaa tcgcttcttt acgaatgcat acttccggtt cgtcgttatc
    12721 cagcagcccg gcgcaggttt caatcagctg cccgctttca ttgccattaa cccaggtagc
    12781 gacacggaac tgacgaatca gaaccacgct ctttttcttc gcgttgtaca ggaggatcgt
    12841 cgcaccatta ccgcgatcgt aaacttcacg tttatggcgg ataacttcgc catctttgcg
    12901 cgtgagatcg taggtaatgt tgtgcagggt gaaatagtta tcggagagaa ttttgtcttt
    12961 aatgagggtg atttgttgcg tcataccgac tccacagcgc gaaatgaaca attatcttac
    13021 gctgtgaagt cgggtttgtc tgcgcaggct atcagtgaga tttgactgat tttaccccca
    13081 gccagtcaat aatcccctgt gccgcatgac gtccttctgc cattgcggta accaccagat
    13141 ccgcaccacg cacggcgtcg ccaccggcga agattttcgg attggtggtc tggtaacggt
    13201 actggctttc cacatccgcg atgatgcggc cccatttgtc taccgttaca ccgtgcgact
    13261 ccagccacgg catcccgtgt ggattgaagc caaacgccat aatcaccgca tcggctggca
    13321 tgacaaattc actgccctca atcggtacag gtcgccgacg cccctgggca tccggctcgc
    13381 caagacgcgt gcgcaggaaa cgaattccgc aaacgtgacc ttgttcattc agctcaagcg
    13441 ccaccggctg gacgttaaat tcgaagttgg ccccctcttc gcgggcgttc ttcacctctt
    13501 tcttcgagcc tggcatgtta gcttcatcac gacgataagc gcaggtgacg ttactcgcgc
    13561 cgtggcgcag cgcagtacgc acacagtcca tcgcggtgtc gccgccgccc agtaccacga
    13621 cgttaagtcc ggcggtattg ataaacggct cttccggtag ctcttcgagc cccatcacct
    13681 gtttagtgtt ggcaatgagg aacggcagcg cgtcataaac gcccggcgca tcttcattgg
    13741 gtaaacccgc tttcatggaa cggtaagtgc ctacgccaac gaagaccgcg tcgtattgtt
    13801 ccaaaagcga atccaaagag acatctttac ccacttcaca attgagttcg aagtgaatcc
    13861 ccatcgcgct gaagatttcc cgacggcgtg ccagcaggga tttatccagt ttgaaagaag
    13921 gaatgccgaa agtgagcaag ccaccaattt ctggatggcg atcgtacacc gtcaccgcca
    13981 cgccattgcg ggtcagaaca tccgcacagg ccagccctgc cgggcctgcc ccgataatcg
    14041 ccacccgctt gtccactttg gtgacatgac ttaagtcagg acgccaacct ttcgccaacg
    14101 cctgatctga aatatagcgt tcaatgttgc cgatagttac agcaccgtgc tcatcgcgaa
    14161 tagtacaggc accttcacac aaacggtctt gcggacaaac gcgtccggta atttccggca
    14221 gggtgttggt ctggtgagaa agctcgacgg cggcgtcgat gtttccggct ttcaccagtt
    14281 caatccactg cggaatatgg ttatgcagcg ggcaggtcca ttcacaaacg ctgtgctcgc
    14341 cgcacttaag gcagcgcgag gcttcccgtt gtgcctggtc ggcgcgaaat ggcagataaa
    14401 tttcatcaaa accggttttg cgcgcttcaa tcgccagttt atccggctcg ccacgcgcgg
    14461 gcgttgcctg catttgttcg actttactca ttaccggcat ttcttgcgcc gcggtactgg
    14521 catgccacgg ttgatgttcc tgacgcgcgg tgcgcaagcg gcgggatttc gccatgccgg
    14581 agagtgcgac gtcagtgacc agttgcagcg cgtccgccgg gcaattctca acacaggcag
    14641 gaccgttttc gcgccccgca caaaggtcgc atttatgcgc cgtggctttt acttttcctg
    14701 ccgcgacggg tgtcaggacg atttgcatcg taccaaaagg acaggccacc acgcaggatt
    14761 tacagccaat acacttttgc tgattgacct gaatgctgtc atcaacgtgg ctgattgcgc
    14821 cattagggca gctacgggcg cagggcgcat cttcacaatg gtgacaggtc actgcactac
    14881 gttgctgttg atgtttgata accgtaattc ggggatgaaa atggtgttgg ctcaggacat
    14941 gttgctcatc attgtgagcc atgacacagg cgatttcaca agcatgacaa cccagacatt
    15001 gctgactgtt ggccataata aaacgattca taacgacctt cttttttggt tgtaaaaacc
    15061 ttattcttta tatgagtgtt gttattaccc gacttacagg ggaatcggca atgttcatgt
    15121 gcccagaata agtaactatt tcgctataaa ctgtggcaga tcaaataatc ccatcactga
    15181 ctaaattgtg tttcagctaa ctggaacatt aatgattttt tgtggagaag acgcgtgtga
    15241 ttgttaaacg acccgtctcg gccagtctgg cccgggcctt tttttacatt gtgctgctgt
    15301 cgattctttc cacgggtatc gctctgctaa ctctggcgag cagtttgcgc gacgctgagg
    15361 ctatcaatat tgccggatcg ctacgtatgc agagttaccg cctgggctac gacttgcaaa
    15421 gtggcagtcc acaactcaat gcacatcgcc agttgtttca gcaggcactg cattcaccgg
    15481 tattaaccaa tctcaacgtc tggtatgtgc cagaagcagt aaaaactcgc tatgcgcatc
    15541 tgaatgccaa ctggctggag atgaataatc ggctcagcaa gggcgatttg ccgtggtatc
    15601 aggccaatat taataattat gttaatcaga tagacctgtt cgtgctggct ttacagcact
    15661 acgctgaacg caaaatgctg ctggtggtgg cgatttccct ggctggcggt atcggtattt
    15721 tcacgctggt cttttttact ttgcgccgta tacgccatca ggtggttgcc ccgctgaatc
    15781 agctggttac cgccagtcaa cgtattgaac acgggcagtt cgactcgccg ccgctggata
    15841 ccagtctgcc gaatgagctt ggtctgcttg caaaaacctt taaccagatg tcgagcgagc
    15901 tgcataaatt gtacctttcg ctggaggcgt cagtagaaga aaagacccgc gatctccacg
    15961 aggccaagcg tcgtctggag gtgttgtatc aatgttccca ggcgctgaac accagccaga
    16021 ttgatgtgca ttgtttccgc catattttgc agattgttcg cgacaatgaa gcggctgaat
    16081 atctggagtt aaatgtcggt gaaaactggc ggattagcga agggcaacca aacccggaat
    16141 tgccgatgca gattttaccg gtgacaatgc aagagacggt ttacggcgaa ctgcactggc
    16201 aaaatagtca cgtttcatca tcagaaccgc tgcttaacag cgtttcgtcg atgctgggac
    16261 gcggtttgta ctttaatcag gcgcagaagc attttcagca gttattgttg atggaagaac
    16321 gtgcaaccat cgcccgcgaa ttgcacgact cgctggctca ggtactttct tacttacgta
    16381 tccagttgac gttactgaag cgttcgatac cggaagataa cgccaccgca caaagtatca
    16441 tggccgattt ttcccaggcg ttgaatgatg cttatcggca gttacgcgag ctgttgacta
    16501 ctttccgcct gacgctgcag caggcggatc tcccctccgc gttgagggaa atgctggata
    16561 cgttacaaaa tcaaaccagc gccaaactga ccctcgactg ccgtctgcca accctggcgc
    16621 tggatgcgca aatgcaggtg catttgttgc aaattattcg cgaagcggtg ctgaatgcga
    16681 tgaagcacgc caacgccagc gaaatcgccg tcagttgcgt caccgcgccg gacggcaatc
    16741 acacggttta tatccgtgat aacgggattg gcatcggtga accgaaagag cccgaaggtc
    16801 attatggtct gaatatcatg cgcgaacgcg cggaacggct gggtgggacg ctgacttttt
    16861 cacaaccttc cggcggcggc acgttagtga gtattagctt tcgctccgcg gagggtgagg
    16921 aaagtcagct gatgtaatgc ctcctactga ccaaagaata cttgcactta aggttcagta
    16981 taaaagggca tgataattta cattaactcc tttttttctc cacgattggc tcgtaccttg
    17041 ccgctacagt gaagcaagtc aagcctacaa cgatacgcag aaacacgagg tcctctttta
    17101 atggcgaatt tctttattga tcgccccatt tttgcctggg tgctggcaat cctgttgtgt
    17161 ctgacaggta ccctggcgat tttctcattg cccgttgaac aataccccga tctcgcgcca
    17221 ccgaatgtgc gagtgaccgc taactatccc ggcgcatcgg cccagacgct ggaaaacacc
    17281 gtgacccagg ttatcgagca aaatatgacc ggcctcgata atctcatgta tatgtcatct
    17341 cagagcagtg gcaccggtca ggcatctgtc actttaagtt ttaaagcagg caccgatccg
    17401 gacgaagccg tgcagcaagt acaaaaccag ctgcaatcag ccatgcgaaa gttaccgcag
    17461 gcggtgcaaa atcagggcgt gacggtgcgt aaaaccggcg ataccaatat tctgaccatt
    17521 gctttcgtct ctaccgatgg ttcgatggat aaacaggata ttgccgatta tgttgccagt
    17581 aatattcagg acccgttaag ccgcgtgaat ggcgtcgggg atatcgatgc ctatggttcg
    17641 caatattcca tgcgtatctg gctggacccg gcgaaactca acagtttcca gatgacggct
    17701 aaagatgtca ctgatgccat tgagtcacag aacgcgcaga ttgcggttgg gcaacttggt
    17761 ggtacacctt ccgtcgataa gcaggcgctc aacgccacca ttaacgccca gtcactgctg
    17821 caaacaccag aacagttccg cgatatcacc ttgagggtga atcaagacgg ctcagaggta
    17881 aggctgggcg atgtcgccac cgtcgaaatg ggggcggaga aatacgatta tcttagccgc
    17941 ttcaatggta agccagcctc cgggctgggg gtaaaactgg cctccggcgc aaacgaaatg
    18001 gcgacagcgg agctggtgct caatcgtctc gacgagctgg cgcagtattt cccgcacgga
    18061 ctggaataca aggtggcgta tgaaaccacc tcgtttgtta aagcctccat tgaagacgtg
    18121 gtgaaaacgc tgctggaagc tatcgctctg gttttcctcg ttatgtatct gttcctgcaa
    18181 aacttccgcg ccacgctgat acccactatc gccgtgccgg tggtgttgat gggaaccttc
    18241 tccgtacttt acgccttcgg ttacagcgtc aacaccttaa ccatgttcgc gatggtgctg
    18301 gcgatcggtc tgctggtgga tgacgcaatc gtggtggtgg aaaacgtcga acgtattatg
    18361 agtgaggaag gactcactcc tcgcgaagcc acacgtaaat cgatggggca gatccagggg
    18421 gcactggtcg ggattgcgat ggttctttcg gcggtatttg taccaatggc cttcttcggc
    18481 ggcaccaccg gtgccatcta tcgccagttc tctattacca ttgttgcggc gatggtgctg
    18541 tcagtactgg tagcgatgat cctcactccg gctctgtgtg ccacactact taagccactg
    18601 aaaaaaggtg agcatcacgg gcaaaaaggc ttttttgcct ggtttaacca gatgtttaac
    18661 cgcaacgccg aacgctacga aaaaggggtg gcgaaaattc tccaccgtag cctgcgctgg
    18721 attgtgattt atgtcctgct gcttggcggc atggtgttcc tgttcctgcg tttgccgacg
    18781 tcgttcttac cgctggaaga ccgtggcatg tttactacct cggtacaatt gcccagcggt
    18841 tcaacccaac aacagaccct gaaagtcgtt gagcaaatcg agaaatatta cttcacccat
    18901 gaaaaagaca acatcatgtc ggtgtttgcc actgttggtt ctggccccgg gggtaacggg
    18961 caaaacgtgg cgcgaatgtt tatccgcctg aaagactgga gcgaacgcga cagtaagacc
    19021 ggcacctcat ttgccattat cgagcgtgca acgaaggcgt ttaacaagat taaagaagct
    19081 cgcgttatcg ccagcagccc gccagcaatt agcggtcttg gtagttctgc aggttttgat
    19141 atggagttgc aggaccacgc tggagcaggt cacgatgcgc tgatggcagc acgtaaccag
    19201 ttgctggcgc tggcggcgga aaacccggag ctaacccgtg tgcgccataa cggcctcgat
    19261 gacagtccgc agttgcagat tgatatcgac cagcgtaaag ctcaggcgct gggcgttgct
    19321 atcgacgata ttaacgacac actgcaaacc gcctggggtt cgagctatgt gaatgacttt
    19381 atggatcgcg gtcgcgtgaa gaaagtctat gtgcaggcag ctgcgccgta tcgcatgctg
    19441 ccagatgaca ttaatctctg gtatgtccga aataaagatg gcggcatggt gcccttctct
    19501 gctttcgcga cctcacgctg ggagacaggc tcgccgcgtc tggaacgcta taacggttat
    19561 tctgcggttg agattgttgg ggaagccgca ccgggggtca gtaccggtac ggcgatggat
    19621 attatggaat cgttagtgaa gcagttgcca aacggctttg gtctggagtg gacggcgatg
    19681 tcgtatcagg agcggctttc cggcgcgcag gctccggcgc tgtacgccat ttccttgctg
    19741 gtagtattcc tgtgtctggc ggcattgtat gaaagctggt cggtaccgtt ctcggtaatg
    19801 ctggtcgtgc cgctgggggt tatcggcgcg ctgctggcaa cctggatgcg cgggctggaa
    19861 aacgacgttt acttccaggt gggcctgtta acggtcattg gtttatcggc gaaaaacgcc
    19921 atcctgatcg tcgagtttgc taacgagatg aaccaaaaag gccacgacct gtttgaagcg
    19981 acgctccacg cctgccgtca gcgtttacgc ccgattctga tgacctcgct ggcatttatc
    20041 ttcggcgtat tgccaatggc aaccagcacg ggtgccggtt ccggtggtca gcatgcggtg
    20101 ggtactggcg taatgggcgg gatgatttca gctactattc tggctattta cttcgtgccg
    20161 ctgttctttg tgctggtgcg ccgccgcttc ccgctgaagc cgcgcccgga ataagcaata
    20221 aaaaaggcga catgccaatg tgtcgccttt ttcaactttc cgattaagaa cctgctcagc
    20281 gggttcttgc tgtttatact ttgtctcggg aattacttac gaagcataac ttcgataaag
    20341 tctttccagt tccccagttc acgttcaatc ataacaacct ctcttataat tatgggtatt
    20401 ctacggaaac aatataccgt ggtgaagcta atttactcga ttgctgcgat gactacctcc
    20461 gggggacaaa ccttatgtaa atactatggt cctgcggtga taatttgtat gtgatacaca
    20521 gcaacatttc gagatattca tacggcatct aatactgatt taattctggt taaaata
//
LOCUS       DAZFXT010000081        20569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.081, whole genome shotgun sequence.
ACCESSION   DAZFXT010000081 DAZFXT010000000
VERSION     DAZFXT010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.081"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHKW0_003186"
     CDS             complement(<1..209)
                     /locus_tag="ACHKW0_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7475974.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKW0_003187"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="ACHKW0_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HFO7475975.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKW0_003188"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="ACHKW0_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HFO7475976.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKW0_003189"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="ACHKW0_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HFO7475977.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKW0_003190"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="ACHKW0_003190"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HFO7475978.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKW0_003191"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="ACHKW0_003191"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HFO7475979.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKW0_003192"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="ACHKW0_003192"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HFO7475980.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTYDRVVQF"
     gene            complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKW0_003193"
     CDS             complement(4598..5320)
                     /gene="yheO"
                     /locus_tag="ACHKW0_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7475981.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKW0_003194"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="ACHKW0_003194"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HFO7475982.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKW0_003195"
     CDS             6520..6738
                     /gene="slyX"
                     /locus_tag="ACHKW0_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HFO7475983.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKW0_003196"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="ACHKW0_003196"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HFO7475984.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKW0_003197"
     CDS             complement(7472..7672)
                     /gene="yheV"
                     /locus_tag="ACHKW0_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative zinc ribbon protein"
                     /protein_id="HFO7475985.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKW0_003198"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="ACHKW0_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015503 - glutathione-regulated potassium
                     exporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HFO7475986.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKW0_003199"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="ACHKW0_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HFO7475987.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLVG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKW0_003200"
     CDS             10169..12082
                     /gene="yheS"
                     /locus_tag="ACHKW0_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HFO7475988.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKW0_003201"
     CDS             12082..13104
                     /gene="yheT"
                     /locus_tag="ACHKW0_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HFO7475989.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKW0_003202"
     CDS             13098..13316
                     /gene="yheU"
                     /locus_tag="ACHKW0_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HFO7475990.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKW0_003203"
     CDS             13370..14239
                     /gene="prkB"
                     /locus_tag="ACHKW0_003203"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HFO7475991.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKW0_003204"
     CDS             complement(14294..14698)
                     /gene="yhfA"
                     /locus_tag="ACHKW0_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HFO7475992.1"
                     /translation="MQARVKWVEGLIFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="ACHKW0_003205"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="ACHKW0_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HFO7475993.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKW0_003206"
     CDS             15683..17773
                     /gene="yhfK"
                     /locus_tag="ACHKW0_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccS/YhfK family putative transporter"
                     /protein_id="HFO7475994.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKW0_003207"
                     /gene_synonym="dapC"
     CDS             complement(17840..19060)
                     /gene="argD"
                     /locus_tag="ACHKW0_003207"
                     /gene_synonym="dapC"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HFO7475995.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKW0_003208"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="ACHKW0_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HFO7475996.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKW0_003209"
     CDS             complement(19741..20343)
                     /gene="fic"
                     /locus_tag="ACHKW0_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HFO7475997.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKW0_003210"
     CDS             complement(20333..20500)
                     /gene="yhfG"
                     /locus_tag="ACHKW0_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HFO7475998.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
BASE COUNT         5059 a         5625 c         5089 g         4796 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agaaaacggc tcgtcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatat tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcataggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacagc gccatctggt cgccctggaa taaacgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccgc cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata caacacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgagat
     8641 tgagcgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaacg
     8761 taccgagcgc catcgacagc cccagcgcgt ccataaacaa tgcggaaccc aacaccaaca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaaa cggaataccg
     8881 gacgcagtaa atagcgcccg ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg aacccgccaa caacggcact aacgccagtg
     9001 ccgggatcac cgccagatcc tgaaacagca gaaccgaaaa cccgagctgc ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaacgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgacaacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa gaccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccatggac
     9361 caattgcaat tcctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtgactcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaaggga cgcagaacat cgctcatcgg
     9661 gtagcgattc aatgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccacca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcataaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcgccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aaccctgggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgccgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttctc
    10621 cggtggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggcaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagtttgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgatggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc agaccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagctgcgt gcgcaaaccc agccgctgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc ggaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcgtggct ggaagcccag gagcagcttg agcagatgct
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaatttagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctaca aactatgctg
    12181 ccgcgtttgt ttcgtcgtca ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagaccccg cacaggcgaa acataaaccg
    12301 cgtttagtgg tgtttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaggctaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgacgaagc
    13681 cgtaccgtgg aatcaggtac cggggacatt caccccctgg caacctttac cggaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctcgatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg ggtctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatagaa accacatcga tggcactgca accccccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctgatgac
    14641 cagaggcgga ttcgcccaga aaaattaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg caggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgaccctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataat tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacaaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tacccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagc
//
LOCUS       DAZFXT010000082        20323 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.082, whole genome shotgun sequence.
ACCESSION   DAZFXT010000082 DAZFXT010000000
VERSION     DAZFXT010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20323
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.082"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..1099
                     /gene="ybeQ"
                     /locus_tag="ACHKW0_003211"
     CDS             122..1099
                     /gene="ybeQ"
                     /locus_tag="ACHKW0_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sel1 family TPR-like repeat protein YbeQ"
                     /protein_id="HFO7475999.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1169..1651)
                     /gene="ybeL"
                     /locus_tag="ACHKW0_003212"
     CDS             complement(1169..1651)
                     /gene="ybeL"
                     /locus_tag="ACHKW0_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HFO7476000.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFIRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1886..4468
                     /gene="leuS"
                     /locus_tag="ACHKW0_003213"
     CDS             1886..4468
                     /gene="leuS"
                     /locus_tag="ACHKW0_003213"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HFO7476001.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4483..5064
                     /gene="lptE"
                     /locus_tag="ACHKW0_003214"
     CDS             4483..5064
                     /gene="lptE"
                     /locus_tag="ACHKW0_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HFO7476002.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5064..6095
                     /gene="holA"
                     /locus_tag="ACHKW0_003215"
     CDS             5064..6095
                     /gene="holA"
                     /locus_tag="ACHKW0_003215"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HFO7476003.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6097..6738
                     /gene="nadD"
                     /locus_tag="ACHKW0_003216"
     CDS             6097..6738
                     /gene="nadD"
                     /locus_tag="ACHKW0_003216"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HFO7476004.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6762..7373
                     /gene="cobC"
                     /locus_tag="ACHKW0_003217"
     CDS             6762..7373
                     /gene="cobC"
                     /locus_tag="ACHKW0_003217"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043755 - alpha-ribazole phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HFO7476005.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            7633..7950
                     /gene="rsfS"
                     /locus_tag="ACHKW0_003218"
     CDS             7633..7950
                     /gene="rsfS"
                     /locus_tag="ACHKW0_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HFO7476006.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7954..8421
                     /gene="rlmH"
                     /locus_tag="ACHKW0_003219"
     CDS             7954..8421
                     /gene="rlmH"
                     /locus_tag="ACHKW0_003219"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HFO7476007.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8452..10353
                     /gene="mrdA"
                     /locus_tag="ACHKW0_003220"
     CDS             8452..10353
                     /gene="mrdA"
                     /locus_tag="ACHKW0_003220"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HFO7476008.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVV
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10356..11468
                     /gene="mrdB"
                     /locus_tag="ACHKW0_003221"
                     /gene_synonym="rodA"
     CDS             10356..11468
                     /gene="mrdB"
                     /locus_tag="ACHKW0_003221"
                     /gene_synonym="rodA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HFO7476009.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11479..12567
                     /gene="rlpA"
                     /locus_tag="ACHKW0_003222"
     CDS             11479..12567
                     /gene="rlpA"
                     /locus_tag="ACHKW0_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HFO7476010.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12707..13918
                     /gene="dacA"
                     /locus_tag="ACHKW0_003223"
     CDS             12707..13918
                     /gene="dacA"
                     /locus_tag="ACHKW0_003223"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HFO7476011.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            14029..14292
                     /gene="ybeD"
                     /locus_tag="ACHKW0_003224"
     CDS             14029..14292
                     /gene="ybeD"
                     /locus_tag="ACHKW0_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein YbeD"
                     /protein_id="HFO7476012.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14393..15034
                     /gene="lipB"
                     /locus_tag="ACHKW0_003225"
     CDS             14393..15034
                     /gene="lipB"
                     /locus_tag="ACHKW0_003225"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HFO7476013.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIVPRLLENILALLNNPDFEYITA"
     gene            15293..16246
                     /gene="ybeF"
                     /locus_tag="ACHKW0_003226"
     CDS             15293..16246
                     /gene="ybeF"
                     /locus_tag="ACHKW0_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbeF family transcriptional regulator"
                     /protein_id="HFO7476014.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINLTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16455..17420
                     /gene="lipA"
                     /locus_tag="ACHKW0_003227"
     CDS             16455..17420
                     /gene="lipA"
                     /locus_tag="ACHKW0_003227"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HFO7476015.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17521..17724)
                     /gene="tatE"
                     /locus_tag="ACHKW0_003228"
     CDS             complement(17521..17724)
                     /gene="tatE"
                     /locus_tag="ACHKW0_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HFO7476016.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17853..18641)
                     /locus_tag="ACHKW0_003229"
     CDS             complement(17853..18641)
                     /locus_tag="ACHKW0_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HFO7476017.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGELLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18734..19117
                     /gene="crcB"
                     /locus_tag="ACHKW0_003230"
     CDS             18734..19117
                     /gene="crcB"
                     /locus_tag="ACHKW0_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HFO7476018.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19172..19381)
                     /gene="cspE"
                     /locus_tag="ACHKW0_003231"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19172..19381)
                     /gene="cspE"
                     /locus_tag="ACHKW0_003231"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7476019.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19556..20116)
                     /gene="pagP"
                     /locus_tag="ACHKW0_003232"
     CDS             complement(19556..20116)
                     /gene="pagP"
                     /locus_tag="ACHKW0_003232"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HFO7476020.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5139 a         5122 c         5102 g         4960 t
ORIGIN      
        1 ttgcgaggct cgtttattta tacttttctg acgcgacata atacgcataa aataaggctt
       61 acgcaaaaat ggaacacata aaaatataat atgttttatc tgcaaccgta aggaaatgat
      121 aatgattttc acgtcaagtt gctgcgataa tttatcaata gatgagatta tcgaacgtgc
      181 tgaaaaaggc gattgcgagg ctcaatatat tgttgggttt tattataatc gcgatagcgc
      241 aattgattct ccggacgacg aaaaagcctt ttactggctg aagctggccg ctgagcaagg
      301 tcattgtgaa gcacagtatt ccttagggca aaagtatacc gaggataaaa gccgtcataa
      361 agataatgag caagccatct tctggctgaa aaaagctgcc ctacaaggcc atactttcgc
      421 ttccaacgcc cttggctgga cactggatcg tggagaagcc cccaattata aagaagcggt
      481 tgtctggtat cagatagccg cggagagcgg aatgtcttat gcgcaaaata atcttgggtg
      541 gatgtacaga aatggcaacg gagtcgcaaa agactatgcg ctggcatttt tctggtacaa
      601 acaagctgca ttacaaggcc atagtgacgc gcaaaacaat ctggccgatc tttatgaaga
      661 cggaaaaggc gttgctcaaa acaagacact cgccgcattc tggtatttga aaagcgcaca
      721 gcagggtaat cggcacgccc agtttcaaat tgcatgggat tataacgctg gcgaaggggt
      781 ggaccaggac tataagcaag cgatgtactg gtatctgaag gctgccgctc aggaaagcgt
      841 cggcgcttac gtcaacatcg gttatatgta taaacacgga caaggcgttg agaaagatta
      901 tcaggctgcc tttgaatggt ttacgaaagc cgctgaatgc aatgacgcca ctgcctggta
      961 taacctggcc attatgtatc actacggaga aggaagacct gtcgatctcc gacaggctct
     1021 cgacctgtat cgtaaagttc agtcatccgg aaccagagat gtcagtcagg aaattcgtga
     1081 gactgaagat ttactgtaga gataaaaaca agggccgggg aatttcattc ggcccatatt
     1141 ctctttattg ctgtgcgaaa tcagactatt acggctcaaa cgggcgtctc tggaactggt
     1201 catgaccaca tttcgggcat agcgtcagca cttccggtgt gtagatcggg agatggaagt
     1261 gacatttctc gcagaccaga tttcccagcc cgaccacttc tccgctgtga taaaccccat
     1321 gatgattgag gtcctggaaa acttcgcgcc attcaagctg cgttttatcg gtgatatctg
     1381 ccagctcctg ccataagctt tctttaatca cccgtataaa gacgctgtca gattcttctt
     1441 tcaggctctc ttcatagctc atggcgaact cttccaggtc acgtcttaca gctcgcgtca
     1501 gctcatcgac ctcggttcgc gttaactccc ctgtttttat tacgcgctcg cgcgcctgtt
     1561 ccaccagtgc gtcgatatca cgttcgccat tgcgcaggcg ttcgctcagt gacgcgacca
     1621 gttcacggta atattgagca accttgttca tcgtttcatc tcccgggtcg ttaactgact
     1681 ataataatag acgttctttg ttccttgctt tgctaatacg cccacaaact ctgtaaatta
     1741 atgtatgaat aatccgggca tcatttcaac gtcgagtgcg cgaaaggctg ttttgacgcg
     1801 gacgtttggg ctatgctatg cggatctgaa aaaccacctc aacgctacat ttgtagccgt
     1861 attgaaaaca ggaccactgg ctgccatgca agagcaatac cgcccggaag agatagaatc
     1921 caaagtacag cttcattggg atgagaagcg cacatttgaa gtaaccgaag acgagagcaa
     1981 agagaagtat tactgcctgt ctatgcttcc ctatccttct ggtcgactac acatgggcca
     2041 cgtacgtaac tacaccatcg gtgacgtgat cgcccgctac cagcgtatgc tgggcaaaaa
     2101 cgtcctgcag ccgatcggct gggacgcgtt tggtctgcct gcggaaggcg cggcggtgaa
     2161 aaacaacacc gctccggcac cgtggacgta cgacaacatc gcgtatatga aaaaccagct
     2221 caaaatgctg ggctttggtt atgactggag ccgcgagctg gcaacctgta cgccggaata
     2281 ctaccgttgg gaacagaaat tcttcaccga gctgtataaa aaaggcctgg tatataagaa
     2341 gacttctgcg gtcaactggt gcccgaacga ccagaccgta ctggcgaacg aacaagttat
     2401 cgacggctgc tgctggcgct gcgataccaa agttgaacgt aaagagatcc cgcagtggtt
     2461 tatcaaaatc actgcttacg ctgacgagct gctcaacgat ctggataaac tggatcactg
     2521 gccagacacc gttaaaacca tgcagcgtaa ctggatcggt cgttccgaag gcgtggagat
     2581 caccttcaac gttaacgact atgacaacac gctgaccgtt tacactaccc gcccggacac
     2641 ctttatgggt tgtacctacc tggcggtagc tgcgggtcat ccgctggcgc agaaagcggc
     2701 ggaaaataat cctgaactgg cggcctttat tgacgaatgc cgtaacacca aagttgccga
     2761 agctgaaatg gcgacgatgg agaaaaaagg cgtcgatact ggctttaaag ctgttcaccc
     2821 attaacgggc gaagaaattc ccgtttgggc agcaaacttc gtattgatgg agtacggcac
     2881 gggcgcagtt atggcggttc cgggtcacga ccagcgcgac tacgagtttg cctctaaata
     2941 cggcctgaac atcaaaccag ttatcctggc tgctgacggc tctgagccag atctttctca
     3001 gcaagccctg actgaaaaag gcgtgctgtt caactctggc gaattcaacg gtcttgacca
     3061 tgaagcggcc ttcaacgcca tcgccgataa actgactgcg atgggcgttg gcgagcgtaa
     3121 agtgaactac cgcctgcgcg actggggtgt ttctcgtcag cgttactggg gcgcgccgat
     3181 tccgatggtg acgctggaag acggtaccgt aatgccgacc ccggacgacc agctgccggt
     3241 gatcctgccg gaagatgtgg tgatggacgg cattaccagc ccgattaaag cagatccgga
     3301 gtgggcaaaa actaccgtta acggtatgcc agcgctgcgt gaaaccgaca ctttcgacac
     3361 ctttatggag tcctcctggt actatgcgcg ctacacttgc ccagagtaca aagaaggtat
     3421 gctggattcc gaagcggcta actactggct gccggtggat atctacattg gtggtatcga
     3481 acacgccatt atgcacctgc tctacttccg cttcttccac aaactgatgc gtgatgcagg
     3541 catggtgaac tctgacgaac cagcgaaaca gttgctgtgt cagggtatgg tgctggcaga
     3601 tgccttctac tatgttggcg aaaacggcga acgtaactgg gtttccccgg ttgatgctat
     3661 cgttgaacgt gacgagaaag gccgtatcgt gaaagcgaaa gatgcggcag gccatgaact
     3721 ggtttatacc ggcatgagca aaatgtccaa gtcgaagaac aacggtatcg acccgcaggt
     3781 gatggttgaa cgttacggcg cggacaccgt tcgtctgttt atgatgtttg cttctccggc
     3841 tgatatgact ctcgaatggc aggaatccgg tgtggaaggg gctaaccgct tcctgaaacg
     3901 tgtctggaaa ctggtttacg agcacacagc aaaaggtgat gttgcggcac tgaacgttga
     3961 tgcgctgact gaagatcaga aagcgctgcg tcgcgatgtg cataaaacga tcgctaaagt
     4021 gaccgatgat atcggccgtc gtcagacctt caacaccgca attgcggcga ttatggagct
     4081 gatgaacaaa ctggcgaaag caccaaccga tggcgagcag gatcgcgctc tgatgcagga
     4141 agcgctgctg gccgttgtcc gtatgcttaa cccgttcacc ccgcacatct gcttcacgct
     4201 gtggcaggaa ctgaaaggcg aaggcgatat cgacaacgcg ccgtggccgg ttgctgacga
     4261 aaaagcgatg gtggaagact ccacgctggt cgtggtgcag gttaacggta aagtccgtgc
     4321 caaaatcacc gttccggtgg acgcaacgga agaacaggtt cgcgaacgtg ctggccagga
     4381 acatctggta gcaaaatatc ttgatggcgt taccgtgcgt aaagtgattt acgtaccagg
     4441 taaactcctc aatctggtcg ttggctaagc gcgggaggaa gcgtgcgata tctggcaaca
     4501 ttgttgttat ctctggcggt gttaatcacc gccgggtgtg gctggcatct gcgtgatacc
     4561 acgcaggttc cttccactat gaaggtcatg atcctggact caggcgatcc gaacgggcca
     4621 ttaagccgtg cggtgcgtaa ccagttacgt ctgaatggtg tcgagttgct tgataaagaa
     4681 accacgcgta aggacgttcc atccctgcgt ttgggtaaag tgagcatcgc gaaagatacc
     4741 gcatcggtat tccgtaacgg tcaaacagca gagtatcaga tgatcatgac ggtgaatgcg
     4801 accgtgttga tccccggccg tgatatctac ccgattagtg ccaaagtctt ccgttcgttc
     4861 tttgataacc cgcaaatggc gttagcgaaa gataacgaac aagacatgat cgtaaaagag
     4921 atgtacgacc gtgctgccga acagctgatt cgtaagctgc caagcatccg tgctgcggat
     4981 attcgttccg acgaagaaca gacgtcgacc acaacggata ctccggcaac gcctgcacgc
     5041 gtctccacca cgctgggtaa ctgatgattc ggttgtaccc ggaacaactc cgcgcgcagc
     5101 tcaatgaagg gctgcgcgcg gcgtatcttt tacttggtaa cgatcctctg ttattgcagg
     5161 aaagccagga cgctgttcgt caggtagctg cggcacaagg attcgaagaa caccacactt
     5221 tttccattga tcccaacact gactggaatg cgatcttttc gttatgccag gctatgagtc
     5281 tgtttgccag tcgacaaacg ctattgctgt tgttaccaga aaacggaccg aatgcggcga
     5341 tcaatgaaca acttctcaca ctcaccggac ttctgcatga cgacctgctg ttgatcgtcc
     5401 gcggtaataa attaagcaaa gcgcaagaaa atgccgcctg gtttactgct cttgcgaatc
     5461 gcagcgtgca ggtgacctgt cagacaccgg agcaggctca gcttccccgc tgggttgctg
     5521 cgcgcgcaaa acagctcaac ttagaactgg atgacgctgc aaatcaggtg ctctgctact
     5581 gttatgaagg taacctgctg gcgctggctc aggcactgga gcgtttatcg ctgctctggc
     5641 cagacggcaa attgacatta ccgcgcgttg aacaggcggt gaatgatgcc gcgcatttca
     5701 ccccttttca ttgggttgat gctttgttga tgggaaaaag taagcgcgcg ttgcatattc
     5761 ttcagcaact gcgtctggaa ggcagcgaac cggttatttt gttgcgcaca ttacaacgtg
     5821 aactgttgtt actggttaac ctgaaacgcc agtctgccca tacgccactg cgtgcgttgt
     5881 ttgataagca tcgggtatgg cagaaccgcc ggggcatgat gggcgaggcg ttaaatcgct
     5941 taagtcagac gcagttacgt caggccgtgc aactcctgac acgaacggaa ctcaccctca
     6001 aacaagatta cggtcagtca gtgtgggcag agctggaagg gttatctctt ctgttgtgcc
     6061 ataaacccct ggcggacgta tttatcgacg gttgatatga aatctttaca ggctctgttt
     6121 ggcggcacct ttgatccggt gcactatggt catcttaaac ccgtggaaac gctggcgaat
     6181 ttgattggtc tgacgcgggt cacaatcatc cctaataatg ttcctccgca tcgtccccag
     6241 ccggaagcga acagcgtgca gcgtaaacac atgcttgaac tggcgattgc cgacaagcca
     6301 ttatttactc ttgatgaacg cgagctaaag cgcaatgccc cctcttacac tgcgcaaaca
     6361 ctgaaagagt ggcggcagga acaagggccg gacgtgccgc tggcgtttat tattggtcag
     6421 gattcattgc tgacctttcc gacctggtac gaatacgaaa cgatactcga caatgcacat
     6481 ttgatcgtct gtcggcgtcc aggttaccca cttgaaatgg cgcaaccgca ataccagcaa
     6541 tggctggaag atcatttgac acataacccg gaagatcttc accttcagcc tgccggtaaa
     6601 atttatctgg ctgaaacgcc gtggtttaac atctcggcga ccatcatccg cgaacgtttg
     6661 caaaacggtg aatcgtgtga ggatttattg ccggaaccgg tactgactta cattaaccaa
     6721 caaggcttgt atcgctgata cctgtcgtta cggagggcat gatgcgactg tggttaattc
     6781 gtcatggcga aacgcaagcg aatgtcgatg gtctgtacag cggtcatgcg cccacacccc
     6841 tgaccgcacg cggtattgag caagcgcaaa atctgcatac gctgctacat ggtgtttcct
     6901 ttgatctggt tttatgcagt gaactggaac gggcacagca taccgcgcga ctggttctca
     6961 gtgaccgcca gctccccgtt catatcatcc ctgaactcaa cgaaatgttt tttggcgact
     7021 gggagatgcg acatcatcgc gacctcatgc aagaagatgc cgaaaactat agcgcgtggt
     7081 gcaatgactg gcagcatgca atccccacga acggtgaagg atttcaggca ttttcgcaac
     7141 gtgtggaacg ctttatcgca aggcttagtg aatttcagca ctatcagaat attttagtcg
     7201 tcagccatca gggtgtactg agtctgttaa tcgcccgttt aattggcatg cctgccgagg
     7261 ctatgtggca ttttcgcgtt gaccaggggt gttggagcac cattgatatc aaccaaaaat
     7321 tcgcgacgct acgcgtcctc aatagccgtg ccatcggggt cgagaatgca tgacttttct
     7381 gtttttttac gggtaagccg caacgaccat tgacagaccc gggcaggctg atattctccg
     7441 cagccagact tttttcgcca gacacgactt tgtagaaatt gttttacaaa aatggcgatg
     7501 caatctgcgg cgcggggtgg gatgatagcc cactttcgaa agccgattcg gcgacaattg
     7561 tcccgaaatc gcctctggtt caggtatact gacagaccat ttttatctat ttgattcacc
     7621 cagggggaaa acttgcaggg taaagcactc caggattttg ttatcgacaa aattgatgac
     7681 ctcaaaggtc aggacatcat cgccttagac gttcaaggca aatccagcat caccgactgc
     7741 atgatcatct gtacgggtac gtccagccgt catgttatgt ccattgctga ccacgttgtg
     7801 caggagtctc gcgcagcggg cctgttaccg ctcggcgtag aaggtgaaaa cagcgccgac
     7861 tggattgtcg tggatttggg cgatgtgatt gtccatgtca tgcaggaaga gagccgtcgc
     7921 ctgtatgaac tggaaaaact ctggagttaa tgcgtgaagc tgcaacttgt cgccgtggga
     7981 acgaaaatgc cggactgggt acaaaccggt tttaccgagt acctgcgtcg ttttccgaaa
     8041 gatatgccct tcgagctgat tgaaattccg gccggaaaac gcggcaagaa tgcggacatc
     8101 aagcgcatac tcgacaaaga gggtgagcag atgttggcgg ccgcaggcaa aaaccgcatt
     8161 gtcaccctcg atattccagg caagccctgg gatacgccgc agttagccgc tgagctggaa
     8221 cgctggaagc tggatggtcg cgacgtcagt ctactgattg gcgggcctga agggttgtcg
     8281 cctgcctgta aagcggcggc ggagcagagc tggtcgctgt cgacgcttac cctcccccat
     8341 ccgctggttc gcgtgctggt cgcagagagt ctgtaccggg cgtggagcat caccaccaac
     8401 catccttatc accgtgagtg ataagggagc tttgagtaga aaacgcagcg gatgaaacta
     8461 cagaactctt ttcgcgacta tacggcagag tccgcgctgt ttgtgcgccg ggcgctggtc
     8521 gcctttttgg ggattttgct gctgaccggc gtgcttatcg ccaacctgta taatctgcaa
     8581 attgttcgct ttaccgacta ccagacccgc tctaatgaaa accgcattaa gctggtgcct
     8641 atcgcgccca gccgcggcat tatctacgac cgtaatggta tccctctggc cctcaaccgc
     8701 actatctacc agatagaaat gatgccagag aaagtcgata acgtgcagca aacgctggac
     8761 gctttgcgca gcgtggttga tctgaccgat gacgatattg ctgcattccg aaaagagcgc
     8821 gcacgttcac accgtttcac ctctattccg gtgaaaacta acctgaccga agtacaagta
     8881 gctcgctttg ccgtcaatca gtaccgtttt ccgggtgtcg aagttaaagg ctataaacgt
     8941 cgttactatc cttacggttc ggcgttgacc cacgtcatcg gctatgtgtc gaaaatcaac
     9001 gataaagacg tcgaacgcct gaataatgac ggcaaactgg ccaactatgc ggcaacgcat
     9061 gatatcggta agctgggcat tgagcgttac tatgaagacg tggtgcacgg tcagaccggt
     9121 tatgaagagg ttgaagttaa caaccgtggg cgtgttatcc gccagttaaa agaagtacca
     9181 ccgcaagccg gacacgatat ttacctgacg ctggatctca aactccagca atatattgaa
     9241 acgctgctgg cgggtagccg cgcagctgtg gttgtcaccg atccgcgtac aggtggggtg
     9301 ctggcgctgg tttccacgcc tagttatgac ccaaacctgt ttgttgacgg tatctccagc
     9361 aaagattatt ccgccttgtt gaacgacccg aatacaccgc tggtgaaccg cgccacacag
     9421 ggggtttatc ctcccgcgtc tacagttaaa ccctatgtgg cggtttcggc attgagcgcc
     9481 ggggtgatca cgcgcaatac gacgctgttt gacccaggct ggtggcaact gccaggttcg
     9541 gaaaaacgtt atcgtgactg gaaaaaatgg ggccacgggc gtctgaatgt cacaagatcg
     9601 ctggaagaat ctgcggatac cttcttctat caggtggcct acgatatggg gatcgatcgc
     9661 ctctccgaat ggatgggtaa attcggttat ggtcattaca ccggtatcga cctggcggaa
     9721 gaacgttccg gcaacatgcc tacccgcgaa tggaaacaga aacgctttaa aaaaccgtgg
     9781 tatcagggtg acaccattcc ggttggtatc ggtcagggtt actggacagc gaccccaatc
     9841 cagatgagta aggcactgat gatcctgatt aatgacggta tcgtgaaggt tccgcatttg
     9901 ctgatgagca ccgccgaaga cggcaaacag gtgccatggg tacaaccgca tgaaccgccc
     9961 gtcggcgata ttcattccgg ttactgggag ctggcgaaag acggtatgta cggtgttgct
    10021 aaccgcccta acggtacggc gcataaatac tttgccagcg caccgtacaa aattgcggcg
    10081 aaatctggta ccgctcaggt cttcggtctg aaagcgaacg aaacctataa tgcgcacaaa
    10141 attgccgagc gtttacgtga ccacaaactg atgaccgcct ttgcgccata caacaatccg
    10201 caagtggctg tcgccatgat tctggagaac ggcggcgcgg gtccggcggt tggtacgctg
    10261 atgcgccaga tcctcgacca cattatgctg ggtgataaca acaccgatct gcctgcggaa
    10321 aatccagcgg ttgccgcagc ggaggaccat taatcatgac ggataatccg aataaaaaaa
    10381 cattctggga taaagtccat ctcgatccca caatgctgct gattttactg gcattgctgg
    10441 tttacagcgc cctggttatc tggagcgcca gcggtcagga tattggcatg atggagcgca
    10501 aaatcggcca aatcgcgatg ggtctggtca tcatggtggt gatggcgcaa attcctccac
    10561 gcgtttatga aggctgggcc ccctatctct atatcatctg tattattttg ctggtggcgg
    10621 tagatgcttt cggtgccatc tctaaaggtg ctcaacgctg gctggacctc ggtattgttc
    10681 gttttcagcc gtcggaaatt gccaaaatag ccgtaccact gatggttgcg cgctttatca
    10741 accgcgacgt ttgcccgcca tcgttgaaga acactggcat cgcgctggtg ctgatattta
    10801 tgcccacgct gctggtggct gcacagcctg acctgggaac atcaatcctc gttgcgcttt
    10861 ccggtctgtt tgtactgttc ctctctggcc ttagctggcg tctgattggc gtcgcagtag
    10921 tgctggtggc ggcgttcatt ccgattctgt ggttcttcct gatgcatgat taccagcgcc
    10981 agcgcgtaat gatgctcctg gacccggaat cagacccact cggcgcgggc tatcacatta
    11041 ttcagtctaa aattgctatt ggctccggcg gattacgcgg caaaggctgg ctgcacggca
    11101 ctcagtcaca gcttgaattt ctccccgaac gccataccga ctttatcttc gcggtactgg
    11161 cggaagagct gggattagtg ggcattttga ttctgctcgc tctctacatt ctgctgatca
    11221 tgcgcgggct gtggatagcc gccagagcgc aaaccacctt tggtcgcgtc atggctggtg
    11281 gcttaatgct gatattattc gtttatgtct tcgtaaatat tggtatggta agcggtattc
    11341 tgccggttgt aggggttccg ctcccactgg tcagttatgg aggatcggcg ctaattgtgc
    11401 tgatggctgg gttcgggatt gtaatgtcaa tccacaccca caggaaaatg ttgtcgaaaa
    11461 gcgtgtaaga ggtgcgcaat gcgtaagcag tggctcggga tctgcatcgc ggcaggaatg
    11521 ctcgcggcat gtacaagcga tgatggtcag caacagacgg taagtgtacc gcagcctgcg
    11581 gtatgtaacg gccccatagt tgaaattagc ggggcggatc cgcgtttcga accactgaac
    11641 gcgacggcaa atcaggatta ccagcgcgac ggtaaaagct acaaaatcgt gcaggatccg
    11701 tctcgattta gccaggcggg actggcggca atctatgatg ccgaaccagg cagtaacctg
    11761 acggcctctg gcgaagcttt cgatccgaca cagctgacgg cggcccatcc aacgcttccg
    11821 atccccagct acgccagaat cactaacctg gctaacgggc gaatgatcgt ggtgcgcatt
    11881 aatgatcgcg gcccttacgg caacgaccgc gttatttcgc tttctcgcgc ggcagctgac
    11941 cgtcttaaca cgtcaaacaa caccaaagtt cgtatcgatc cgattattgt cgcccaggat
    12001 ggttcgcttt ctggtcctgg tatggcttgt accacagtcg ccaaacagac ttacgccctg
    12061 ccagcacctc ccgatttaag cggtggcgcg ggaacaagtt cagtgtctgg cccgcagggt
    12121 gacattcttc cggtcagtaa ttcgacgcta aaaagcgaag atccgaccgg cgcgccggta
    12181 accagtagcg gtttcctcgg cgctccaacg accttagcgc ctggtgtact ggaaggcagc
    12241 gaaccgacgc ctgcgccaca gcccgttgtt acagctccgt cgacaacgcc tgcaacctcg
    12301 cctgcaatgg tgacaccgca agccgcctcg caaagcgcca gcggcaactt tatggtgcaa
    12361 gtcggggccg ttagcgatca ggctcgtgcg caacagtacc aacagcaact gggacagaag
    12421 ttcggcgtcc ccggtcgcgt aactcaaaat ggcgcggtct ggcggatcca gcttggccca
    12481 ttcgccagca aagccgaagc cagcaccttg cagcaacgtt tgcaaaccga agcccaatta
    12541 cagtcattta ttaccaccgc gcagtagcgt aaagcaggca tctgatgtgt caatttctgt
    12601 aaatgacgtt aacaaagtca cgcggaaagt cagatgcctg ccggtagtgc atttgctata
    12661 gtagggcact ttttttaatt ccatcacgga tgtcgtagtt cagaccatga ataccatttt
    12721 ttccgctcgt atcatgaagc gcctggcgct caccacggct ctttgcacag cctttatctc
    12781 tgctgcacat gccgatgacc tgaatatcaa aactatgatc ccgggtgtac cgcagatcga
    12841 tgcggagtcc tacatcctga ttgactataa ctccggcaaa gtgctcgccg aacagaacgc
    12901 agatgtccgc cgcgatcctg ccagcctgac caaaatgatg accagttacg ttatcggcca
    12961 ggcaatgaaa gccggtaaat ttaaagaaac tgatttagtc actatcggca acgacgcatg
    13021 ggccaccggt aacccggtgt ttaaaggttc ttcgctgatg ttcctcaaac cgggcatgca
    13081 ggttccggtt tctcagctga tccgcggtat taacctgcaa tcgggtaacg atgcttgtgt
    13141 cgccatggcc gattttgccg ctggtagcca ggacgctttt gttggcttga tgaacagcta
    13201 cgttaacgca ctgggcctga aaaataccca cttccagacg gtacatggtc tggatgctga
    13261 tggtcagtac agctccgcgc gagatatggc gctgatcggc caggcattga tccgtgacgt
    13321 accgaatgaa tactcgatct ataaagaaaa agaatttacg tttaacggta ttcgccagct
    13381 gaaccgtaac ggcctgttat gggataacag cctgaatgtc gacggcatca aaaccggaca
    13441 cactgacaaa gcaggttaca accttgttgc ttctgcgact gaaggccaga tgcgcttgat
    13501 ttctgcggtg atgggcggac gtacttttaa aggccgtgaa gccgaaagta aaaaactgct
    13561 aacctggggc ttccgtttct tcgaaaccgt taacccactg aaagtaggta aagagttcgc
    13621 ctctgaaccg gtttggtttg gtgattctga tcgcgcttcg ttaggggttg ataaagacgt
    13681 gtacctgacc attccacgtg gtcgcatgaa agatctgaaa gccagctatg tgctgaacag
    13741 cagtgaattg catgcgccgc tgcaaaagaa tcaggtcgtc ggaactatca acttccagct
    13801 tgatggcaaa acgatcgagc aacgcccgct ggttgtgttg caagaaatcc cggaaggtaa
    13861 cttcttcggc aaaatcattg attacattaa attaatgttc catcactggt ttggttaaaa
    13921 attgaacact tgaaagtgta atttccgtcc ccatatacta agcatcagta aaaaaactcc
    13981 cgccttctgg cgggagttgc tatttaatta cgttacgccg gagctgacat gaaaaccaaa
    14041 cttaacgaac tgcttgaatt ccctactcct tttacttaca aagttatggg gcaggcgtta
    14101 cctgagctgg ttgatcaggt ggttgaagtg gtacagcgcc atgcgccagg tgactacacc
    14161 ccaacggtaa aaccaagcag caaaggcaac taccactcgg tatctatcac tatcaacgcc
    14221 actcatatcg agcaggttga aacactgtat gaagaactgg gcaaaatcga tattgtccgc
    14281 atggttctgt aactcgcttc tccgttaccc ggctcctggt cgggtaactc ccctctcctc
    14341 gctgtgatat acttttcccc cacttttact cattctccac ggagatgccg ttttgtatca
    14401 ggataaaatt cttgtccgcc agctcggtct tcagccttac gagccaatct cccaggctat
    14461 gcatgaattc accgataccc gcgatgatag tacccttgat gaaatctggc tggtcgagca
    14521 ctatccggta ttcacccaag gtcaggcagg aaaagcggag cacattttaa tgccgggtga
    14581 tattccggtg atccagagcg atcgcggtgg gcaggtgact tatcacgggc cgggacaaca
    14641 ggtgatgtat gtgttgctta acctgaaacg ccgtaaactc ggtgtgcgtg aactggtgac
    14701 cttgcttgag caaacagtgg tgaataccct ggctgaactg ggtatagaag cgcatcctcg
    14761 ggctgacgcg cctggtgtct atgtcgggga aaagaaaatt tgctcactgg gtttacgaat
    14821 tcgacgcggt tgttcattcc acggtctggc attaaacgtc aatatggatc tttcaccatt
    14881 tttacgtatt aatccttgtg ggtatgccgg aatggaaatg gctaaaatat cacaatggaa
    14941 acccgaagcg acgactaata atattgttcc acgtttactg gaaaatattt tagcgctact
    15001 aaacaatccg gacttcgaat atattaccgc ttaattccat acctcaatgg cccaatttac
    15061 actgggtcat tactcataat acctgctaat gctttacatc tccttcgcgt ttgtatattt
    15121 tctttatcac ttgcagaaat gcggtgccac gccttgctcc gtaactcacc ccattgatta
    15181 acctattgaa atagttgatt atggtgagca aaagcgttcc gttccaatta gacataagat
    15241 tattgtaatt ttttaaatat tatttttcca tgaataaaat tggagtaagt gcgtggatag
    15301 taataatcaa attgaaccct gtttaagcag aaagtcgtca gaaggtaaac cacaaatatt
    15361 tacaacctta cgaaatattg atcttaacct tctgactatt tttgaagctg tatatgtaca
    15421 taaagggatc gttaatgcag cgaaagtgct taatctgacc ccctcggcaa tcagtcagtc
    15481 tattcagaaa ctgcgcgtta tattccctga cccattgttt attcgcaaag gccagggtgt
    15541 cactcctacc gcatttgcga tgcatctaca tgagtatatc agtcagggcc ttgagtccat
    15601 tcttggcgcg ctggatatcg aaggaagcta tgataagcaa cgaacgataa ctattgcgac
    15661 cactccctcg gtcggagccc tggtccttcc tgtcatctat cgggcgatta aaactcacta
    15721 tccgcagctt ttactgcgca acccgcccat cagcgacgcg gaaaaccaac tcagtcagtt
    15781 tcaaaccgat ctcatcatcg ataacatgtt ttgcaccaat cgtacggtgc aacatcatgt
    15841 tctgttcacc gacaatatgg tgttaatttg ccgcgaggga aatccactac tctctttaga
    15901 agatgacaga gagactatcg acaacgctgc gcatgtactt ctgttaccgg aagagcaaaa
    15961 tttcagcggt ctgcggcaga gagttcaaga aatgtttccg gaccggcaaa tcaatctcac
    16021 cagctacaac attttgacaa tcgctgcact ggttgccaac agtgacatgt tagcgattat
    16081 tcccagccgt ttttataacc tgtttagccg ctgctggccg ctggaaaaac tgccttttcc
    16141 gtccttaaat gaggagcaaa tagatttctc tatccactac aacaaattca gcctgcgtga
    16201 tcccatcctg cacggagtaa tagatgttat ccgtaatgca ttttgaaaaa gtattgttac
    16261 agccagatca cagcgcagaa cgataaaatg gcacaatcca acaacaattt tacattttcg
    16321 cgaccgcttt ggctgctttc aggtccgttt caatgatata ctgccagtcg ttaattcaaa
    16381 aatagttgat aattacaaca atctattgaa ttgaaacgct ttccttcgta attcgcaact
    16441 ggaacacgca cgctatgagt aaacccattg tgatggaacg cggtgttaaa taccgcgatg
    16501 ccgataagat ggcccttatc ccggttaaaa acgtggcaac agagcgcgaa gccctgctgc
    16561 gcaagccgga atggatgaaa atcaagcttc cagcggactc tacacgtatc cagggcatca
    16621 aagccgcaat gcgcaaaaat ggcctgcatt ctgtctgcga ggaagcctcc tgccctaacc
    16681 tggcggaatg cttcaaccac ggcacagcaa cgtttatgat cctcggcgct atttgtaccc
    16741 gccgttgtcc gttctgtgat gttgcccacg gtcgcccggt agctcctgat gccaatgaac
    16801 cagtgaaact ggcgcagacc attgccgata tggcgctgcg ttatgtggtt atcacctccg
    16861 ttgaccgtga tgacctgcgc gatggcggtg cccagcactt tgcggattgc attactgcca
    16921 ttcgggaaaa aagcccgcaa atcaaaattg aaactctggt gccggatttc cgtggtcgta
    16981 tggatcgtgc tctggatatt ctgactgcaa cgccaccaga tgtgttcaac cataacctgg
    17041 aaaacgtacc gcgtatttac cgtcaggtac ggcctggtgc agattacaac tggtcgctga
    17101 agctgctgga acgctttaaa gaagcgcatc cggaaatccc gaccaagtct ggtctgatgg
    17161 tgggactggg tgaaaccaat gaagaaatta ttgaggtaat gcgcgacctg cgccgtcatg
    17221 gtgtgacgat gttaacgctg gggcaatatt tgcagccaag ccgccatcac ctgccggttc
    17281 aacgttacgt tagcccggat gagttcgacg aaatgaaagc cgaagcgctg gcgatgggct
    17341 ttacccatgc tgcatgcggt ccgtttgtcc gctcttctta ccacgccgat ttgcaggcga
    17401 aagggatgga agttaagtaa tcctgtaata attacttaca tctgtctgat aaaaaacccg
    17461 ctttttgaag cgggtttttt gtatcgaaca agatattgag ggagcgtcct gctcgccacg
    17521 tcactcttta tgagagagct tttcagcctg aagatcaacg tctgcgcctt ttttcgccgc
    17581 agcatcgtca tcattcatcg ccttcttgaa ccctttaatg gccgctccaa ggtctccgcc
    17641 cagcgtacgt aacttcttag tcccaaacag cagaacgacc agcgccgcaa ctactagcag
    17701 tttggtaata ctaatctcac ccatagatac cttcttgaca tataaccggc aacagaacgc
    17761 gccttaaaaa ccagagaata ttaacggtca ttcgacgcgc gcacacaata gcaatctgta
    17821 acaaggtgaa tcaagcgccg agtaaaaaaa catcataata attgcggcgg cgcaaagcga
    17881 cggttgttta agacgggcag ttgcgcgcgc acctgacgca cacgttcggg cgtcacttcc
    17941 gccataatga gtgcaggcat ttctgacgct gccgcaatgg tgacgccaaa gggatctata
    18001 atccggcttt gaccgatatt tttgttcccg cactcccccg ccgccaccat ataacaggtg
    18061 gtatccagcg cacgagcggc aagcaacgtt gaccaatgat gctctttgag cggcccgcga
    18121 acccaggcgg caggaagtac caggatttca gctccctgta atgcctgtgc cagcgccagc
    18181 tctggaaagc gtaagtcata acaggtcatc agaccgacct tcatcccctc cacctcgagt
    18241 aacggagcga tttcattacc agcatcaaca cggcgtgatt cctgaatggc aaatgcatca
    18301 tagagatgca atttggcata acgggcgacg atgtttcctg cctgtagcgc caccagcata
    18361 ttccatgcgc gccccggcgt tgaaggaaca tgaatcgtca gaattgtcgt catcatgtta
    18421 cgtttacttt ctcgccgtaa acgtccgagg agttcgcctt ccagcagctg tgctgattta
    18481 accgatagat ctgcatcatg atcatcgcgc gccagcaatg cttccggcag gacgaacagc
    18541 gatacgtcgt tttccgccgc ctgcgccatc aacgaggcac aaatctcagc gttcttttcc
    18601 cacacagatg taacagcaaa ctgtcctgcg gcaaccagca ttgccacctc ctgtcagagt
    18661 cgtaaaattc gccaacgacg attattcggc gttacactta tcactcatac aaatcaaata
    18721 gcaggatttt gcagtgttac aacttctttt agcagttttt attggcggtg gtacgggaag
    18781 cgtggcgaga tggctgttaa gtatgcgatt taacccgctg catcaggcga ttccgttggg
    18841 gacgctggca gcaaacctga ttggggcatt catcatagga atgggattcg cctggttcag
    18901 caggatgacg aacattgatc cagtgtggaa agtattaatc accaccggat tttgtggcgg
    18961 tctaacaacc ttctcaacat tttcggcaga agtggtgttt ttgttacaag agggccgctt
    19021 tggctgggcg ttactgaacg ttttcgtcaa ccttctgggg tcttttgcca tgacagcact
    19081 ggctttctgg ctgttttcgg cctcaaccgc acactaaaga ccaaaaaaaa acccgctgat
    19141 taagcgggtt ttgaattctt gctgacgtat cttacagagc gattacgttt gcagcagaag
    19201 ggcctttggc accgttagtg atttcgaact ctacgcgctg accttcagca agagttttaa
    19261 aaccattagt ctggattgca gagaagtgta cgaacacgtc tttgctgccg tcttccggag
    19321 taatgaaacc gaatcctttg gactcattaa accacttaac gttaccttta atcttagaca
    19381 tcaaaattac ctttacatga aaaatagaca caaatgctgt gtcggttacc agtacaccaa
    19441 ttgtggtacg ctttgtccag tgcaacttta ctaaaaagtg ataaaagtcg cgttattttt
    19501 gatgttactt attttagcta ttgattttaa agaagttact aaaacttcat ttgtctcaaa
    19561 actgaaagcg catccaggca aagtacacat tgccattgtt gtaggtaccc ggaatgtagg
    19621 tcatctgaaa agtcactggg ccataaccca cggaggccaa tggcagtaga accgggagag
    19681 ggatgtaatt ccagttatcg cgtgccgtta cgccagcggt gaatcccaga cctaaatgaa
    19741 aattttcatc cgccagcggt cgccaggtac tttcccatcc gtatccggca atcggttccc
    19801 atttgttcca cgagtcctta aatgccatgg catacaggcc atgccagttt cctttttcat
    19861 cccaacgcga caggccaaaa ccgccacccc acggtcgctc gttatagcga tcggtttttt
    19921 ctttgtcgta agcgaaacgt gcatgccagg tgatggcagg aatatataaa tcataatgtt
    19981 caggctgttg ccaggtttgt gcaatatttt ctctaaacgt tgtcatccac tcatctgcgt
    20041 tagcaaaaac tttaccaacg ctgattaact gaataaaaac aaaggaaaag atagcgacat
    20101 atttactcac gttcatttgt gaccataaaa catttatcaa aaatctacta ctagcatagc
    20161 aaagctacaa ttaacataac cttaatagac ccaacataaa gaataatctg aatagctgct
    20221 ctatttattt ccgcttgttc aagttaaata tttccgcgca tcgattaaag atgatgcgtc
    20281 gttttctgcg atgggaatag tcaaaaaaga aaaaccaagt ctt
//
LOCUS       DAZFXT010000083        20217 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.083, whole genome shotgun sequence.
ACCESSION   DAZFXT010000083 DAZFXT010000000
VERSION     DAZFXT010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20217
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.083"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..889
                     /gene="yieE"
                     /locus_tag="ACHKW0_003233"
     CDS             8..889
                     /gene="yieE"
                     /locus_tag="ACHKW0_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YeiE"
                     /protein_id="HFO7476021.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            1094..2563
                     /gene="lysP"
                     /locus_tag="ACHKW0_003234"
     CDS             1094..2563
                     /gene="lysP"
                     /locus_tag="ACHKW0_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HFO7476022.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPH
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            2856..4847
                     /gene="cirA"
                     /locus_tag="ACHKW0_003235"
     CDS             2856..4847
                     /gene="cirA"
                     /locus_tag="ACHKW0_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HFO7476023.1"
                     /translation="MFRLNPFVRVGLCLSAISCAWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(4879..5715)
                     /gene="yeiG"
                     /locus_tag="ACHKW0_003236"
     CDS             complement(4879..5715)
                     /gene="yeiG"
                     /locus_tag="ACHKW0_003236"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HFO7476024.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSRYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            5973..6641
                     /gene="folE"
                     /locus_tag="ACHKW0_003237"
     CDS             5973..6641
                     /gene="folE"
                     /locus_tag="ACHKW0_003237"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HFO7476025.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            6658..7815
                     /gene="yeiB"
                     /locus_tag="ACHKW0_003238"
     CDS             6658..7815
                     /gene="yeiB"
                     /locus_tag="ACHKW0_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein YeiB"
                     /protein_id="HFO7476026.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGPAISKTSR"
     gene            7957..8997
                     /gene="galS"
                     /locus_tag="ACHKW0_003239"
     CDS             7957..8997
                     /gene="galS"
                     /locus_tag="ACHKW0_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HFO7476027.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMTKLATELALQGAAGNIDSRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            9277..10275
                     /gene="mglB"
                     /locus_tag="ACHKW0_003240"
     CDS             9277..10275
                     /gene="mglB"
                     /locus_tag="ACHKW0_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HFO7476028.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            10336..11856
                     /gene="mglA"
                     /locus_tag="ACHKW0_003241"
     CDS             10336..11856
                     /gene="mglA"
                     /locus_tag="ACHKW0_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043211 - ABC-type carbohydrate
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HFO7476029.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            11872..12882
                     /gene="mglC"
                     /locus_tag="ACHKW0_003242"
     CDS             11872..12882
                     /gene="mglC"
                     /locus_tag="ACHKW0_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HFO7476030.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(12953..14188)
                     /gene="preA"
                     /locus_tag="ACHKW0_003243"
     CDS             complement(12953..14188)
                     /gene="preA"
                     /locus_tag="ACHKW0_003243"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HFO7476031.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(14182..15420)
                     /gene="preT"
                     /locus_tag="ACHKW0_003244"
     CDS             complement(14182..15420)
                     /gene="preT"
                     /locus_tag="ACHKW0_003244"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HFO7476032.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLLLFEHSDVEIAVDFLQRARQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTINTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(15739..15978)
                     /gene="yeiS"
                     /locus_tag="ACHKW0_003245"
     CDS             complement(15739..15978)
                     /gene="yeiS"
                     /locus_tag="ACHKW0_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HFO7476033.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(15981..16700)
                     /gene="sanA"
                     /locus_tag="ACHKW0_003246"
     CDS             complement(15981..16700)
                     /gene="sanA"
                     /locus_tag="ACHKW0_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HFO7476034.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(16850..17734)
                     /gene="cdd"
                     /locus_tag="ACHKW0_003247"
     CDS             complement(16850..17734)
                     /gene="cdd"
                     /locus_tag="ACHKW0_003247"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HFO7476035.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEEALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(17864..18559)
                     /gene="yohK"
                     /locus_tag="ACHKW0_003248"
     CDS             complement(17864..18559)
                     /gene="yohK"
                     /locus_tag="ACHKW0_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HFO7476036.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(18556..18954)
                     /gene="yohJ"
                     /locus_tag="ACHKW0_003249"
     CDS             complement(18556..18954)
                     /gene="yohJ"
                     /locus_tag="ACHKW0_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HFO7476037.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            19193..20143
                     /gene="dusC"
                     /locus_tag="ACHKW0_003250"
     CDS             19193..20143
                     /gene="dusC"
                     /locus_tag="ACHKW0_003250"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HFO7476038.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGDIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIDKLR"
BASE COUNT         4929 a         5027 c         5275 g         4986 t
ORIGIN      
        1 agcgactatg cacatcaccc tccggcagtt ggaagttttt gcagaagtat tgaaaagtgg
       61 atcaaccacc caggcgtcgg tgatgctggc gttgtcgcaa tcagcagtga gcgcagcctt
      121 gaccgacctg gaagggcagc ttggcgtgca actgtttgat cgcgtgggga aaagactggt
      181 tgttaatgaa cacgggcggc tgctctatcc gcgtgcgttg gcattgcttg aacaggcggt
      241 tgaaatcgaa caactgtttc gcgaagacaa cggcgcgatt cgtatctatg ccagtagtac
      301 catcggtaac tacattctgc ctgcagttat cgcccgttat cgccatgatt atccgcagtt
      361 gccgattgaa cttagcgttg ggaacagcca ggacgtgatg caagcggtgc tggatttccg
      421 cgttgatatt ggttttattg aaggaccgtg ccacagcact gaaattattt ctgaaccgtg
      481 gctggaagac gagctggtgg ttttcgccgc gccgacttcg ccgttggccc gtggtccggt
      541 caccttagag cagctggccg ctgcgccgtg gatcctgcgt gaacgcggtt ccggcacgcg
      601 ggagattgtc gattatctat tgctgtcaca tttaccgaag tttgagatgg cgatggaatt
      661 aggtaactcc gaggcaatca aacatgcggt gcgtcatggg ttgggaatta gttgcctgtc
      721 gcgacgggtg attgaagatc aattgcaggc aggcacatta agtgaagttg cggtccctct
      781 gccgcgcctg atgcgtacgt tgtggcgtat acatcatcgg caaaaacacc tttccaacgc
      841 gctacggcgc tttctggact attgcgatcc cgcgaatgta ccgcgttaag ttgctgtaca
      901 agaacatgct ggtgctgtgt cgatttcgtg acgcagcgcc ctcagcatgc attcgccaga
      961 aaagagattg gctgctttac ttataatccc tgggcgatca tgaaggtgtc ttataaccgt
     1021 gtatttctgc cggaaggatt gccaatcgtc tgctacaatc gcgcctcatt tttaagatgg
     1081 atagcatttt tgtatggttt ccgaaactaa aaccacagaa gcgccgggct tacgccgtga
     1141 attaaaggcg cgtcacctga cgatgattgc cattggcggt tccatcggta caggtctttt
     1201 tgttgcctct ggcgcaacga tttctcaggc aggtccgggc ggggcattgc tctcgtatat
     1261 gctgattggc ctgatggttt acttcctgat gaccagtctc ggtgaactgg ctgcatatat
     1321 gccggtttcc ggttcgtttg ccacttacgg tcagaactat gttgaagaag gctttggctt
     1381 cgcgctgggc tggaactact ggtacaactg ggcggtgact atcgccgttg acctggttgc
     1441 agctcagctg gtcatgagct ggtggttccc ggatacaccg ggctggatct ggagtgcgtt
     1501 gttcctcggc gttatcttcc tgctgaacta catctcagtt cgtggctttg gtgaagcgga
     1561 atactggttc tcactgatca aagtcacgac agttattgtc tttatcatcg ttggcgtgct
     1621 gatgattatc ggtatcttca aaggcgcgca gcctgcgggc tggagcaact ggacaatcgg
     1681 cgaagcgccg tttgctggtg gttttgcggc gatgatcggc gtagctatga ttgtcggctt
     1741 ctctttccag ggaaccgagc tgatcggtat tgctgcaggc gagtccgaag atccggcgaa
     1801 aaacattcca cgcgcggtac gtcaggtgtt ctggcgaatc ctgttgttct atgtgttcgc
     1861 gatcctgatt atcagcctga ttattccgta caccgatccg agcctgctgc gtaacgatgt
     1921 taaagacatc agcgttagtc cgttcaccct ggtgttccag cacgcgggtc tgctctctgc
     1981 ggcggcggtg atgaacgcag ttattctgac ggcggtgctg tcagcgggta actccggtat
     2041 gtatgcgtct actcgtatgc tgtacaccct ggcgtgtgac ggtaaagcgc cgcacatttt
     2101 cgctaaactg tcgcgtggtg gcgtgccgcg taatgcgctg tatgcgacga cggtgattgc
     2161 cggtctgtgc ttcctgacct ccatgtttgg caaccagacg gtatacctgt ggctgctgaa
     2221 cacctccggg atgacgggtt ttatcgcctg gctggggatt gccattagcc actatcgctt
     2281 ccgtcgcggt tacgtattgc agggacacga cattaacgat ctgccgtacc gttcaggttt
     2341 cttcccactg gggccgatct tcgcattcat tctgtgtctg attatcactt tgggccagaa
     2401 ctacgaagcg ttcctgaaag ataccattga ctggggcggc gtagcggcaa cgtatattgg
     2461 tatcccgctg ttcctgatta tttggttcgg ctacaagctg attaaaggaa ctcacttcgt
     2521 acgttacagc gaaatgaagt tcccgcagaa cgataagaaa taagtttcct cccttccttg
     2581 ctaagccctc tcaaccgaga gggctttttc aattccattt ccctgacaaa tcatgcggat
     2641 ataaaattta acatttgggt tgataattgt tatcgtttgc attatcgtta cgccgcaatc
     2701 aaaaaaggct gacaaatcag aggctgttcc ggctttctgg gatgatcacc tgcatacaaa
     2761 taagtccacc gcgatgctgc cgtacgcaag gggacgtgaa gaagatgtga gcgataaccc
     2821 attttatttt cgtagttacc tcatggagat atggaatgtt taggttgaac cctttcgtac
     2881 gggtcgggct gtgtttgtcc gctatttctt gtgcatggcc tgtgttagcg gtcgatgatg
     2941 atggcgaaac gatggttgtc actgcatctt ccgttgaaca aaacctcaaa gatgcacccg
     3001 ccagtatcag cgtcattacc caggaagacc tgcagcgaaa accggtacag aatctgaagg
     3061 atgtcctcaa agaagtgcct ggcgtacaac tgacgaacga aggggataac cgtaagggcg
     3121 ttagtattcg tggtctggac agcagctaca ccctgattct cgtcgacggt aaacgcgtta
     3181 actcccgcaa tgccgtcttc cgccacaatg atttcgatct gaactggatc ccggtcgatt
     3241 ccatcgaacg tattgaagtg gttcgtggcc cgatgtcgtc gctatacggc tccgatgcgc
     3301 tcggcggtgt agtgaatatc atcaccaaaa aaatcggtca gaaatggtcg ggtaccgtta
     3361 ccgtcgatac caccattcag gaacatcgcg atcgcggtga tacctataac ggtcagttct
     3421 ttaccagtgg accattaatt gatggtgtgc tgggaatgaa agcttacggc agcctggcaa
     3481 aacgtgaaaa ggatgacccg caaaactcaa cgaccaccga taccggagaa acgccgcgta
     3541 ttgaaggatt ctccagccgc gacggcaatg tcgaatttgc ctggacaccg aatcaaaatc
     3601 acgattttac tgccggatac ggtttcgacc gtcaggatcg tgattccgac tcgctggaca
     3661 aaaaccgcct ggaacgccag aactactccg tcagccataa tgggcgttgg gattacggca
     3721 ccagcgaact gaaatactac ggtgagaaag tcgagaacaa aaaccctggc aacagcagcc
     3781 cgataacttc cgaaagcaat acggtcgacg gcaaatacac gttgccgctg acggcgatta
     3841 atcagtttct cacggttggc ggtgaatggc gtcacgacaa acttagcgat gcggtgaacc
     3901 tgaccggggg aaccagctcc aaaacgtctg ccagccagta cgcgctgttt gtggaagatg
     3961 aatggcggat cttcgagccg ctggcgctga cgaccggcgt gcgtatggac gatcacgaaa
     4021 cctacggtga acactggagt ccgcgtgcct acctggttta taacgccacc gacaccgtaa
     4081 cggtgaaagg gggctgggcg acggcattta aagcaccttc tctgttgcaa cttagccctg
     4141 actggacgag caattcctgc cgtggcgcat gtaagattgt gggtagcccg gatctgaaac
     4201 cagaaaccag cgaaagttgg gagctggggc tttactacat gggtgaagaa ggctggctgg
     4261 aaggggttga atccagcgtt accgttttcc gtaacgatgt gaaagatcgc atcagcatca
     4321 gccgtacgtc tgacgtcaac gctgcaccgg gctaccaaaa ctttgttggt tttgagacgg
     4381 gcgctaacgg acggcgcata ccggtattta gctactacaa cgttaacaaa gctcgtattc
     4441 agggcgtgga aaccgaactg aaaattccgt tcaacgatga atggaaactg tcgatcaact
     4501 acacctacaa cgatggtcgt gatgtcagca acggtgaaaa caaaccgcta tccgatctgc
     4561 cgttccatac tgctaacggt acgctggact ggaaaccgct ggcgctggaa gactggtcat
     4621 tctatgtttc tggtcactat accgggcaga aacgcgccga cagcgcgacg gctaaaacac
     4681 cgggcggtta taccatctgg aataccggcg cggcctggca ggtgactaaa gacgtcaaac
     4741 tgcgcgcagg cgtgctgaac cttggcgaca aggatctcag tcgtgacgac tacagctata
     4801 acgaagacgg acgtcgttac tttatggcag tggattatcg cttctgatga gaagatgccc
     4861 ggcgaaccgg gcggactttc acttcagtaa atactgcgca tggaagcgca ggtgatcctc
     4921 tataaaagag gcgatgaagt agtaactgtg atcatatccc ggctgaatac gcagcgtcat
     4981 cggccacgct ttctggcgcg cggcttctgc cagtaccgca ggttgcaact ggtcggcaag
     5041 aaactgatca ttatcgccct gatcgataag cgtcgggatc gcatcctgcg cgttactggc
     5101 atacatcagc gcgcaactgt cccattccag ccatgcattt ttgtcctcac ctaaatagcg
     5161 gctaaacgct ttgattcccc acgggacgct gcacggattc acaattggcg caaaggccga
     5221 aacgctggtg tatttacccg gatttttcag cgccataatc agcgcaccgt gaccacccat
     5281 tgagtgaccg ctaatggcgc agcggtcgct gacattaaat tgcgactgaa ccagcgccgg
     5341 taattcatcg cgcagataat catacatccg gtaatgcgtc gcccacggcg gttgcgtggc
     5401 attaagataa aagcctgcgc cctggcctaa atcgtagcca tcgtcgttgg caaccttttc
     5461 gccgcgcggg ctggtgtctg gcatcaccag tacaatcccc agttctgccg ccacgcgctg
     5521 ggcacccgcc ttggtggtga agttctcgtc attgcaggtt aatccggaaa gccagtacag
     5581 cactggtggc ggggtgtgat cacgaggggg tgggagaaag atactgaacg tcatcgggca
     5641 gtttaaggta ctggagtcgt gtcgccagcg ttgctgccag ccttcaaaac agcggtgctc
     5701 ttcgagcatt tccatgcgtg gctccttgtt gtgttgtttg caatttcttc atcataccgt
     5761 ttattcaatg tgctgtgagt aactttcact tccgtatttg cataacgatg ttttaacatc
     5821 tgctgatgaa aggcagcggc aattacaata attatcgctg tgaatactgg attatgtgcg
     5881 ccgcctcacg cacaataatc aggctgtaaa tcagcttaat aactttgccc ccacgcaggg
     5941 cggaggcgtc acacctgcag gagaaatcat aaatgccatc actcagtaaa gaagcggccc
     6001 tggttcatga agcgttagtt gcgcgaggac tggaaacacc gctgcgcccg cccgtgcatg
     6061 aaatggataa cgaaacgcgc aaaagcctta ttgctggtca tatgaccgaa atcatgcagc
     6121 tgctgaatct cgacctggct gatgacagtt tgatggaaac gccgcatcgc atcgctaaaa
     6181 tgtatgtcga tgaaattttc tccggtctgg attacgccaa cttcccgaaa atcaccctca
     6241 ttgaaaacaa aatgaaggtc gatgaaatgg tcaccgtgcg cgatatcact ctgaccagta
     6301 cctgtgaaca ccattttgtt accatcgatg gcaaagcgac ggtggcttat atcccgaaag
     6361 attcggtgat cggtctgtca aaaattaacc gtatcgtgca gttctttgcc cagcgtccgc
     6421 aggtgcagga acgtctgacg cagcaaattc ttattgcgct acaaacgctg ctgggcacca
     6481 ataacgtggc tgtctcgatc gacgcggtgc attactgcgt gaaggcgcgt ggcatccgcg
     6541 atgcaaccag tgccacaaca acgacctctc ttggtggatt gttcaaatcc agtcagaata
     6601 cgcgccacga gtttctgcgc gctgtgcgtc atcacaactg attaaaaggc aggaaccatg
     6661 gagcgcaacg tcacgctcga ttttgttcgc ggcgtcgcca ttctggggat cctgctatta
     6721 aacatcagcg cctttgggct accaaaggcg gcttatctca atcccgcctg gtacggtgct
     6781 attacgccgc aggatgcatg gacctgggca tttcttgatc tcatcggcca ggtgaaattc
     6841 ctcacgcttt ttgcgctgct gtttggtgct ggactgcaaa tgttgctgcc ccgtggcaga
     6901 cgctggatcc agtcgcggtt aacgctgtta gtcttattgg gctttattca cggtttattg
     6961 ttctgggacg gcgatattct gctggcttac gggctggtgg gcttaatctg ctggcggctg
     7021 gtgcgcgatg cgccatcggt aaaaagcctg tttaataccg gcgtcatgct ttatctggtg
     7081 gggcttggcg ttttgctgtt attggggctg atttccgaca gccagaccag ccgcgcctgg
     7141 acgccggatg catcggctat tttgtatgaa aaatactgga agcttcacgg cggcgttgat
     7201 gcgatcagta atcgtgccga tggtgttggc aacagtttac tggcactggg cgcacagtat
     7261 ggctggcaac tggctgggat gatgctcatt ggtgcggcgc tgatgcgcag cggctggctg
     7321 aaagggcagt tcagcttacg tcactatcgt cgtactggtt ttgtgctggt ggcgattggg
     7381 gtgaccatta accttcctgc catcgccctg caatggcagc tggactgggc gtatcgctgg
     7441 tgtgccttct tacttcaaat gccgcgggaa ctgagtgcgc cgtttcaggc gattggctat
     7501 gcgtcgctgt tttatggctt ctggccgcaa ttaagccgct ttaagctggt gctggcaatc
     7561 gcctgcgtcg gacggatggc gctgaccaac tatctattgc aaacgctgat ttgtaccacg
     7621 cttttttacc acctcggttt gtttatgcat tttgaccgcc tggagctgct ggcgtttgtt
     7681 attccggtat ggctggcgaa tatcctcttc tctgttatct ggctgcgtta cttccgccag
     7741 gggccggtgg aatggctctg gcgtcagtta actttgcgtg ctgccggacc ggcaatatct
     7801 aaaacatcaa gataacgatc tggatcacat tcgttaacaa aacggctgta accgtttcca
     7861 ttgctgtgac tcgattcacg aagtcctgta ttcagtgctg acaaaatagc cgccagcaag
     7921 cagtcattta ctgcaatctc ataacaggta gtgaatatga tcaccattcg tgatgtagcg
     7981 cgtcaggctg gcgtctctgt ggcaacggtt tcccgggtgc tcaataacag cacgctggtc
     8041 agtgccgaca cgcgtgaagc agtaatgaaa gccgtgagtg agctggatta tcggccaaac
     8101 gccaatgccc aggcgctggc aactcaggtt agcgacacca ttggcgtggt ggtgatggac
     8161 gtttctgatg cgtttttcgg tgcgctggta aaagcggtgg atctggtcgc ccagcagcat
     8221 cagaaatacg tgctaatcgg caatagctat catgaagcgg aaaaagagcg tcacgccatt
     8281 gaggtgttaa ttcgccagcg ttgtaatgcg ttgattgttc actcaaaagc attgagtgac
     8341 gatgaactgg cgcaatttat ggataacatt cccggtatgg tgttaatcaa ccgcgttgtg
     8401 ccggggtacg cccatcgttg cgtttgcctg gataatctca gcggtgcccg aatggcgacg
     8461 cgcatgttgc tgaataacgg tcatcaacgt attggttatc tttcttccag ccacggcatt
     8521 gaagatgacg ccatgcgtaa agcaggctgg atgagtgcgt tgaaagagca ggatattatt
     8581 ccgccggaaa gctggattgg cactggtacg ccggacatgc cgggcggtga ggcggcgatg
     8641 gttgaactgc tggggcgcaa tctacaactt accgctgtat ttgcttataa cgacaatatg
     8701 gccgctggcg cactgacagc attaaaagat aatggcattg cgattccgtt acatctctca
     8761 atcatcggtt tcgatgatat tcccatcgcc cgttacaccg acccgcaatt aacgaccgtg
     8821 cgttatccca ttgcttcaat gactaaatta gccaccgaac tggccttgca gggggcagca
     8881 ggcaatattg attctcgtgc cagccactgt tttatgccga cgttagtgcg tcgtcattct
     8941 gtcgcaacgc gccagaatgc ggcggcgatc actaactcaa caaatcaggc gatgtaaccg
     9001 ctttcaatct gtgagtgatt tcacagtatc ttaacaatgt gatagctatg attgcaccgt
     9061 tttaacgttg taacccgtat gtaacagtga ataatcactt tcaccgaggt aatagcgtca
     9121 taacaacaat taaagccgtt ttctggagcg ttaccgggca tggaagaacg aattttaaaa
     9181 agtgagcttc ggcgttcagt aacacttcat taactctact gccccgccga gcatttatct
     9241 caagcactac cctgcataag aaaaaccgga gataccatga ataagaaggt gttaaccctg
     9301 tctgctgtga tggccagcat gttattcggt gccgctgcac acgctgctga tactcgcatt
     9361 ggtgtaacaa tctataagta cgacgataac tttatgtctg tagtgcgcaa agctattgag
     9421 caagatgcga aagccgcgcc agatgttcag ctgctgatga atgattctca gaatgaccag
     9481 tccaagcaga acgatcagat cgacgtattg ctggcgaaag gggtgaaggc actggcaatc
     9541 aacctggttg acccggcagc tgcgggtacg gtgattgaga aagcgcgtgg gcaaaacgtg
     9601 ccggtggttt tcttcaacaa agaaccgtct cgtaaggcgc tggatagcta cgacaaagcc
     9661 tactacgttg gcactgattc caaagagtcc ggcattattc agggcgattt gattgctaaa
     9721 cactgggcgg cgaatcaggg ttgggatctg aacaaagacg gtcagattca gttcgtacta
     9781 ctgaaaggtg agccgggcca tccggatgca gaagcacgta ccacttacgt gattaaagag
     9841 ctgaacgata aaggcatcaa aactgaacag ttacagttag ataccgcaat gtgggacacc
     9901 gctcaggcga aagataagat ggacgcctgg ctgtctggcc cgaacgccaa caaaatcgaa
     9961 gtggttattg ctaacaacga tgcgatggca atgggcgcgg ttgaagcgct gaaagcacac
    10021 aacaagtcca gcattccggt gtttggcgtc gatgcgctgc cagaagctct ggcgctggtg
    10081 aaatccggtg cactggcggg caccgtactg aacgatgcta acaaccaggc gaaagcgacc
    10141 tttgatctgg cgaaaaacct ggccgatggc aaaggtgcgg ctgatggcac caactggaaa
    10201 atcgacaaca aagtggtccg cgtaccttat gttggcgtag ataaagacaa cctggctgaa
    10261 ttcagcaaga aataagactg attgggcgca gtcgtttact gcgcccttat tgtatggcca
    10321 acaaggtaaa attatatggt cagctcaacg actccgtctt ccggggaata cttgttggaa
    10381 atgagcggta tcaacaagtc ctttcctggt gttaaggcac ttgataacgt taatttaaaa
    10441 gtccggccac attctatcca tgcattaatg ggggaaaacg gtgcaggaaa atcgacatta
    10501 ttaaaatgcc tgtttggtat ttatcaaaaa gactccggca ccattttatt ccagggtaaa
    10561 gagatcgatt tccattccgc caaagaggca ctggaaaatg gtatttcgat ggtacaccag
    10621 gagttaaacc tggtattaca acgttcggtg atggataaca tgtggctggg gcgatatccc
    10681 accaaaggca tgtttgtcga tcaggacaaa atgtaccgcg aaaccaaagc gatttttgat
    10741 gaactggata ttgatatcga tccgcgtgcg cgcgtcggca cattatccgt ttcgcaaatg
    10801 cagatgatcg aaatcgccaa agcgttttcc tataacgcga aaattgtgat tatggatgaa
    10861 ccgacttctt cgttaaccga aaaagaggtc aatcatctgt tcactattat tcgtaaatta
    10921 aaagagcgcg gctgcggtat tgtttatatc tcgcataaaa tggaagaaat cttccagtta
    10981 tgtgatgaag ttaccgtatt gcgcgacggt cagtggatcg ccaccgaacc gctggcagga
    11041 ctgacgatgg acaagatcat cgccatgatg gttgggcgtt ctcttaatca gcgttttcct
    11101 gacaaagaaa acaagccggg cgaagtcatc ctcgaggtgc gtaacctaac gtcactgcgc
    11161 cagccgtcga ttcgcgatgt ctcgtttgat ctgcataaag gggagatcct cggtattgcc
    11221 gggctggtgg gggcgaaacg taccgatatt gttgagacgt tatttggtat tcgcgagaaa
    11281 tcggctggca ccattacgtt gcacggcaaa cagatcaata accataatgc caacgaagcc
    11341 ataaaccacg gatttgcact ggtaactgag gagcgccgct caacgggaat ttatgcctat
    11401 ctggatattg gttttaactc gttaatttcc aatattcgca actacaaaaa taaagttggt
    11461 ttactggata actcgcggat gaaaagcgat acccagtggg tgattgattc gatgcgggta
    11521 aaaacgccgg gtcatcggac gcaaattggt tcgctctccg gtggtaatca gcaaaaggtg
    11581 attattggtc gctggctact aacgcaacca gaaatattaa tgctcgatga accgacgcgc
    11641 ggtattgatg tcggggcgaa gtttgaaatt tatcagttaa ttgccgaact ggcgaagaaa
    11701 ggcaagggga ttattattat ctcctctgaa atgcctgagt tgttagggat aacagaccgt
    11761 attctggtca tgagcaatgg tctcgtttcc ggaattgtcg atacaaaaac aacaacgcaa
    11821 aacgaaattc tgcgtcttgc gtctttgcac ctttaagatt aggggcttcc catgagtgcg
    11881 ttaaataaga aaagttttct tacttacctg aaagagggcg gtatttacgt cgttctttta
    11941 gttttgctgg cgattattat tttccaggac ccaacatttt taagtctgtt gaacttaagt
    12001 aatattctca cccagtcatc ggtgcgtatt attatcgcgc tcggtgtggc agggttaatt
    12061 gtcacccagg ggaccgatct ttctgctggt cgtcaggtag ggctggcggc agtggtggct
    12121 gcgacattat tgcagtccat ggataacgcc aacaaagtgt tcccggaaat ggcgacgatg
    12181 ccgattgcgc tggttattct gattgtctgt gccattggtg cggtgatcgg tttgatcaac
    12241 ggtctgatta tcgcttatct caacgtgacg ccgttcatta ccacgctcgg cacgatgatc
    12301 atcgtctatg gcatcaactc gctctattac gactttgtcg gggcgtcgcc aatttctggt
    12361 tttgacagtg gcttctctac ctttgctcag ggctttgtcg cgctggggag tttccgtctc
    12421 tcttacatca ccttctacgc gttgattgcg gtggcgttcg tctgggtgtt gtggaacaaa
    12481 acccgcttcg gtaagaacat ttttgccatt ggcggtaacc cggaagcggc aaaagtatct
    12541 ggtgtcaacg tcggcctgaa cctgctgatg atctacgcgt tgtctggcgt gttctatgcc
    12601 tttggcggga tgttagaagc cggacgtatc ggctctgcca ccaacaacct cggctttatg
    12661 tatgagctgg atgctatcgc ggcgtgcgtg gtaggcggtg tatcgttcag cggcggtgtg
    12721 gggacggtga ttggcgtggt gaccggggta attattttta ccgtcatcaa ctatggcctg
    12781 acgtatatcg gcgttaaccc atactggcag tacatcatca aaggggcgat tattatcttc
    12841 gccgtagcgc tggattcact gaaatacgcg cgtaagaagt gatgtgattg tggatattgc
    12901 cggatgcgct gcatccggca atcggtgcca aatgccggat gcgtctctgg ttttacaacg
    12961 ttaccgggtg ttctttctcg cctttcttaa acttcacttc cccgagctca atacaaccca
    13021 ccgggcagac gtgaccacaa agcagacaac ccacacattt ctcggtatta caatgcggtg
    13081 tgcgggtttt ctcgctccat tccatcgcct ggtgaccgcc gtcgtaacag gaaatataac
    13141 agcgtccaca gccaacacat ttatcaagat tgatacgggg atagacaata taactgcggt
    13201 ctaaatcttc cgccgggaca atattgttat tcgccagacc taccatctcc tgcagcgaat
    13261 caaatccctg atcggcgaga taatgcgaca acccgcttgc catatcttcc actatccgat
    13321 acccgtactg catgatgccg gtggtcacct gtaacgttgc tgcgccgagc aataaaaact
    13381 cagccgcatc ctcccaggtt tcaatgccgc cgataccgct gattgggaaa tcgcgcagtt
    13441 ctggatgggt gcgcatctgc tgaatgaagc gcagggcgat cggttttacc gctttaccgg
    13501 aatatccgga aatactcgat tttccgttaa cgatcggcat accgattttc tgattaagat
    13561 cgatattggt gatggattta acggtgttaa tcgccgcaat gccatctgcg ccgccgcgct
    13621 tcgccgccag cgccacttcg cacatatcac cgatattcgg cgtcatcttc gccagcattg
    13681 gcagcgtgga accccgtttc actgcccgac aatatttttc taccagctcc gggctttgcc
    13741 cgacatcgct acccatcgca tgagaagtca tttgcggaca ggagaagtta cactcgatca
    13801 tatccgcgcc agcttcttgc accaggcgcg ccagctcctc ccattgctgc tcattttccc
    13861 ccatgatcga agcgatcaat actttgtccg ggtaatcttc cttcagccga cgcagggcgg
    13921 ccagattctc ttccaacgga tgttcagcaa tctgctccat atttttgaag ccgataaaac
    13981 cggtatcttc tttcaccaga tgatcaaaac gcggcgagac ttcgttggcg ataaaaaagc
    14041 cgatcgtttt aaacaccaca ccgccccaac ctgtgtcgta ggctttggca cacatctcat
    14101 agcagttgcc taccggcgaa gaagagaggc agaacgggtt gggaaacttc acgccgcaaa
    14161 aagtaatcga aagatctttc gttaacatga gcaagctccc tctaaatagt gatgaatcgc
    14221 ctcggcggct tctttcccgg ttttcacggc atagaccacg gttttgtcac cctcaacaat
    14281 atcgcccgca gcaaagactt gcggatcgcg ggtctgataa ttttgtgtat tgatagtgtt
    14341 acgctgcggc tctaactccg caaaggcatc cagtctggca tgctgaccga cggcgagaat
    14401 aattttatct gccgccatcg tcagttcgcc cggtaaccgt acatgcttaa acgtgacttt
    14461 attgccttcc acggctactg gcgtgaatcc atcgatgatc gaaacgccca gctcccgggc
    14521 gctggcaaac tctttttcgc ttgccggaaa ctcatctaac tcttcacgcg ctacgcaggt
    14581 taccgcctga cagccgagaa ctttcagcgt gctggctacg tccatcgcaa catcaccgcc
    14641 gccgataatt aatgcgcttt gtggaatgct gatatcgcct tgcgcctgtc gtgcacgttg
    14701 caggaagtcc acggcaatct caacgtcgct atgctcaaac agcaataacc cggaaccgct
    14761 cgataacccc acggtgacca gtaccgcgcg gttttctgct ttcagctgct caagggtgag
    14821 tgtgttaccg acttcgttgt tgcacttaat ggtcacgccc attttttcaa tacgggcgat
    14881 ctctgcatcc agcactgatt gcggtaaccg gaattgcgga ataccgttac gcaaccagcc
    14941 accggggtgc gcttctttct cataaatcgt gacgtcataa ccctggtttg tcagtgttac
    15001 actggcctgc aatccggcag gacctgcgcc aataatcgcg actttgccga gcgttttagt
    15061 accgggctga taaatttcca tcccggtttg ttgttcaaaa tcagtaacaa aacgctgtaa
    15121 gcggccgata tcaatgggag catcaacacc ggcacgggta caaccgcttt gacataattt
    15181 ctccgtcggg cacactctgg cacaaacggc accgagggca ttattttcgc gaattgtctc
    15241 ggcagcgcct ttaaaattac gaaagtagat tgagcgaata aatttccccg gatcggtctg
    15301 tgccgggcaa gcctgactac agggagcgtc atgacataat aaacagcgag aggcttcttt
    15361 aatcgccagt aaaggcgtaa aagccggagt gagttcatcc agataatttt gttgcggcat
    15421 gtgatcttcc ttaatgctat gagtaattaa ggcgaacgct aaatatactc gtcatacttc
    15481 aagttgcatg tgctgcgtct gcgttcgctc accccagtca cttacttatg taagctcctg
    15541 gggactcact cacttgtcgc cttcctgcaa ctcgaattat ttagagtata aatattgcgt
    15601 gctgatatta aggctatgcg cgtggggaaa tttatactgt gaaaggattc acaaatagct
    15661 gcggttcaga atgttatatc cgcagcttat tgagtgaagt aataattatt ctcaataatt
    15721 aacagaaaag agaaaatatt aacgatagaa aataagataa acaatcatgc cgatagataa
    15781 aattccgaag ccgatatatg ccaccatata agcgaaaaag agtccgggat tttttgctcg
    15841 ccagacatac accggttccg gtgcattttt aacccgttta tcaatcgcgc ctgcgcgcca
    15901 gcctttccat gcttcgcgga aacaaaatat cgggataagg aaaaaaacgg caacgacaaa
    15961 aaactgctgt acatccataa ctactttcct tgtttctttt gtaattcaag taactgttcg
    16021 ggtgtgacgg cgggataccc ctgcgcatct tccggcacct ggtgcatagc cggaataggg
    16081 accagcggcc ctaaaaaacg cggttcacgt ttaaaaatat aaaggtcagc cagcgctccg
    16141 aaacgggcgg caaattcgcg aatacgtact gacagcatat ctttcggtga cggtacggca
    16201 taacactgag cctgaatccc catatgcagc gcaataaata atgcccgctc acagtggaaa
    16261 cgttgggtga taataatgaa atcattagta tcgaaaactt tgcgtgtacg cactatggaa
    16321 tccagcgtac gaaagcctgc gtaatcgaga acaatatctg atgggtcgac accagcagcg
    16381 attaaatctt tgcgcatggt catcggctca ttataacttt gcaatgcgtt atcgccgctc
    16441 agtaatagat aatttacctt accgctgtta taggcattaa tcgctccttg aatgcggtag
    16501 cgataatact gattaattac gccagtacga taatattttg ctgttccgag caccacaccg
    16561 acctggcggt aggggagatc ctgcaattcg tcgtagatat aaggcgcggt tttccagctc
    16621 atccagcgat cgaggccgag cacagtcaac agcagcaagc cgatcaggac taacaggctg
    16681 aggaacacgc gctttaacat gcaggagact cgatctaagg gaaatttcca tcaggctact
    16741 ttacccgctg gttttgcgca agaaacggca aactgtttgc cgggattttc gacaccagga
    16801 gagggctggc aagccggaca aggcgttcac gccgcatccg gcaccaggct taagcgagaa
    16861 gcacgcggtc gatactgtga cagccgagcg ctttcagcgt tgcggaggtg gcatcccact
    16921 gaatcaacgg cgcatcggct ttttctgcca gaaccgcgcg ctggatatcc gggtaatcat
    16981 aacccttgag attcaacaga attaacgctc cctgcaacgg tggcagagtc gggttgaatg
    17041 cggcgttttc agcgtagctg ccactgaaaa tacgaccgtc tttacattcc agcgcgacac
    17101 cgcttggcga cttactgtaa ggcatgtgcg aacggtttgc cgctgcaatc gccgcctgag
    17161 aaagcgcatc accagtcagc gcatagccgt gatcctgctc gtccatcagc agcgttttaa
    17221 tctccagatc tttcggccca aaggcatccg gcagatagtc acgcagcgcg tgtgcctcgc
    17281 ggcccggcag atgaatacgc agatccagac cgctgttcag ttcattcata aactgacggc
    17341 agtgaccaca aggcgtgtag ttaacggtga tggctgcaag cgcttcttca ccactcaacc
    17401 aggcgtggct gatcgcgctt tgttcggcat gaacggtttg ctgcattgtc gcaccaataa
    17461 attccatatt ggcaccgaaa taccaggttc cgctcacacc gcgcgcaatt gcgccaacat
    17521 taaaattcga caatggcgta cgcgcacagg ccgccgccag cggaagtagt gcgaatgcca
    17581 gcgcgtcttc gtccagcccc gttgcgctct tcagcgatga gacttgctcc ccggtcaaca
    17641 aagcggggaa gtacttgtct gccagaatag gttccagtgc agattgcaag ttatccgcaa
    17701 gttgggcaaa agcggtttga aaacgtggat gcatggcgtt gcctcataat catgtaatgg
    17761 gataacagtt tacgaacccg ttatggcttt atatgtgatc tgaatctcat taattatgca
    17821 acgcgtgaaa cttacatcaa aaatgcgcga cgcatcgcaa attttagccc attactgcca
    17881 gaataatcgg gaaaaggaac ggtgcgatca gcgaagtaat tatcccgcat aacaccagcg
    17941 ctagcgaact aaatgcacct tcctgataat ccagctcggc gcagcgcgcc gtaccgaggg
    18001 cgtgcgaggc agtccccatt gccagaccgc gcgcagcttt ggtacgaata cgcatcgcat
    18061 taagcaatgt atggccaaat accgcgccga ggatgccgac gaaaatcacg caaactgcgc
    18121 tgattgccgg aataccgcca atactgccgc caaccgccat tgcaataggc gtggtgactg
    18181 atttcggcag gattgacgcg gcgatttccg gtgaagcgcc catcaacaat gccacggaag
    18241 tccccgtcac catcgcaacc acgctgccga taaaacagat ggtgatgatc gatttccagc
    18301 gcgcgcggat ctggtgtagc tgctcatata aaggataggc cagcgcgacg accgccggtt
    18361 gcagcagatc gtttagcact tcgctacctt taaagtagct gtcgtaagag atgccggtca
    18421 gcattaaaaa aggaatgatc accaccattg ctaccagcaa cgggttaagc aggggaaact
    18481 tataccgtgc cgccagtttg cgggcggcaa aaaagacaat caacgtcagc ggtaatgacc
    18541 accagatatt cgccatcatt cttctgatcc tttctgacct acgactttac gttcaccgtg
    18601 taccagttgt gaactccagc tcaccaccag aaaaaccacc agcgtactga cagcacagga
    18661 aaccactacc gggccaaact gcgcgcggag caaatcaaaa tattgcatga cgcctacgcc
    18721 aatcggcaca aacaatagcg ccatatagcg aatcagtacg tagcaccccg gattgaccca
    18781 tttcgccgga agaatttgca aggccagcag gacaaacagg atcagcatcc cgatgatgct
    18841 gcccggaatg gttaccggta gcagggaagc aatgaaaatg cctgcataca ggcaggcata
    18901 aatcaggacg aaagcgcgta aatattgcca gataatgttc agtgtcttac tcataatcaa
    18961 caccccagat gagatggatt catcatacaa ttaattcgta aaacgtgcta ccgatcacat
    19021 taagaaatca gcgcttattt tttcgctccc cggcattacg caatgcgcaa cgaactcagg
    19081 catacatcaa gcatagagag ggattgcagt ttgcagcaat ccctctttgt agacctttaa
    19141 tccgctgacc ataggcgcta acatagcgcc tcattatttt gcgggtgatg atatgcgtgt
    19201 gttactggca ccgatggagg gagtgcttga ctctctggtg cgtgaattgc tgaccgaagt
    19261 taacgactac gatctgtgca tcaccgagtt tgtccgcgtg gtggatcaac tgctgccggt
    19321 aaaagtcttt catcgcattt gccctgagct acaaaacgcc agccggacac catctggtac
    19381 gctggtgcgc gtgcagttgt taggtcagtt cccacaatgg ctggcagaga acgccgcccg
    19441 tgcggtggag ttaggttcct ggggcgtgga tctcaattgc ggctgcccgt cgaaaacggt
    19501 taacggtagc ggcggcggcg cgacgttact caaagatcct gaactcatct accagggtgc
    19561 aaaagcgatg cgtgaagctg taccggcgca tttgcccgtc agcgtgaaag tgcgtctggg
    19621 ctgggacagc ggtgagaaga aatttgaaat cgccgatgcg gttcaacagg ctggcgctac
    19681 ggagctggtg gtgcatgggc ggacgaaaga gcagggttac cgcgcggagc atattgactg
    19741 gcaggcgatt ggcgatattc gccagcggct gaatattccg gtgattgcca acggtgaaat
    19801 ctgggactgg cagagcgcgc aacaatgcat ggcgatcagc ggctgcgacg cagtgatgat
    19861 tggtcgcggg gcgctcaata ttcccaacct gagccgggta gtaaaatata acgaaccgcg
    19921 aatgccgtgg ccggaggtgg ttgctttgct gcaaaaatat acccgtctgg aaaagcaggg
    19981 cgataccggg ttatatcacg ttgcgcggat taaacagtgg ttgagttatt tgcgtaaaga
    20041 atacgatgaa gcaacgggat tatttcagca tgttcgggtg ttgaataatt cccctgatat
    20101 tgcaagggct attcaggcaa ttgatatcga taagctgcga taatagtgga atcgcagctt
    20161 atcagagaca atgaatcgct tattcagctt catcatcttg ttgcgctcct ttcatga
//
LOCUS       DAZFXT010000084        19671 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.084, whole genome shotgun sequence.
ACCESSION   DAZFXT010000084 DAZFXT010000000
VERSION     DAZFXT010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19671
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.084"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            272..607
                     /gene="yjdM"
                     /locus_tag="ACHKW0_003251"
     CDS             272..607
                     /gene="yjdM"
                     /locus_tag="ACHKW0_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein YjdM"
                     /protein_id="HFO7476039.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            767..1210
                     /gene="yjdN"
                     /locus_tag="ACHKW0_003252"
     CDS             767..1210
                     /gene="yjdN"
                     /locus_tag="ACHKW0_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HFO7476040.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            1343..2131
                     /gene="phnC"
                     /locus_tag="ACHKW0_003253"
     CDS             1343..2131
                     /gene="phnC"
                     /locus_tag="ACHKW0_003253"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HFO7476041.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2156..3172
                     /gene="phnD"
                     /locus_tag="ACHKW0_003254"
     CDS             2156..3172
                     /gene="phnD"
                     /locus_tag="ACHKW0_003254"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7476042.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3278..4057
                     /gene="phnE"
                     /locus_tag="ACHKW0_003255"
     CDS             3278..4057
                     /gene="phnE"
                     /locus_tag="ACHKW0_003255"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HFO7476043.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4078..4803
                     /gene="phnF"
                     /locus_tag="ACHKW0_003256"
     CDS             4078..4803
                     /gene="phnF"
                     /locus_tag="ACHKW0_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HFO7476044.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4804..5256
                     /gene="phnG"
                     /locus_tag="ACHKW0_003257"
     CDS             4804..5256
                     /gene="phnG"
                     /locus_tag="ACHKW0_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HFO7476045.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5253..5837
                     /gene="phnH"
                     /locus_tag="ACHKW0_003258"
     CDS             5253..5837
                     /gene="phnH"
                     /locus_tag="ACHKW0_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HFO7476046.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLAAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5837..6901
                     /gene="phnI"
                     /locus_tag="ACHKW0_003259"
     CDS             5837..6901
                     /gene="phnI"
                     /locus_tag="ACHKW0_003259"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HFO7476047.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6894..7739
                     /gene="phnJ"
                     /locus_tag="ACHKW0_003260"
     CDS             6894..7739
                     /gene="phnJ"
                     /locus_tag="ACHKW0_003260"
                     /EC_number="4.7.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HFO7476048.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7736..8494
                     /gene="phnK"
                     /locus_tag="ACHKW0_003261"
     CDS             7736..8494
                     /gene="phnK"
                     /locus_tag="ACHKW0_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HFO7476049.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8605..9285
                     /gene="phnL"
                     /locus_tag="ACHKW0_003262"
     CDS             8605..9285
                     /gene="phnL"
                     /locus_tag="ACHKW0_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018835 - carbon phosphorus lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HFO7476050.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASS"
     gene            9282..10418
                     /gene="phnM"
                     /locus_tag="ACHKW0_003263"
     CDS             9282..10418
                     /gene="phnM"
                     /locus_tag="ACHKW0_003263"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HFO7476051.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRETIAAL
                     CRVRQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10418..10975
                     /gene="phnN"
                     /locus_tag="ACHKW0_003264"
     CDS             10418..10975
                     /gene="phnN"
                     /locus_tag="ACHKW0_003264"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033863 - ribose 1,5-bisphosphate
                     phosphokinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HFO7476052.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPICLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10962..11396
                     /gene="phnO"
                     /locus_tag="ACHKW0_003265"
     CDS             10962..11396
                     /gene="phnO"
                     /locus_tag="ACHKW0_003265"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HFO7476053.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11398..12156
                     /gene="phnP"
                     /locus_tag="ACHKW0_003266"
     CDS             11398..12156
                     /gene="phnP"
                     /locus_tag="ACHKW0_003266"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HFO7476054.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNDAITLIDAGLHDLADRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPVYGPP
                     DDQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTQKFLLNNHPQVMVIDCSHPPREDAPRNHCDLNTVLALNQVIHSPRV
                     ILTHISHQFDAWLMENALPPGFEAGFDGMEIGVA"
     gene            complement(12303..12632)
                     /gene="yjdP"
                     /locus_tag="ACHKW0_003267"
     CDS             complement(12303..12632)
                     /gene="yjdP"
                     /locus_tag="ACHKW0_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDRRRQL repeat protein YjdP"
                     /protein_id="HFO7476055.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12701..13150)
                     /gene="rpiB"
                     /locus_tag="ACHKW0_003268"
                     /gene_synonym="alsI"
     CDS             complement(12701..13150)
                     /gene="rpiB"
                     /locus_tag="ACHKW0_003268"
                     /gene_synonym="alsI"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional allose-6-phosphate
                     isomerase/ribose-5-phosphate isomerase RpiB"
                     /protein_id="HFO7476056.1"
                     /translation="MKKIAFGCDHVGFILKHEIVAHLVERGVEVIDKGTWSSERTDYP
                     HYASQVALAVAGGEVDGGILICGTGVGISIAANKFAGIRAVVCSEPYSAQLSRQHNDT
                     NVLAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQWRN"
     gene            13509..14399
                     /gene="alsR"
                     /locus_tag="ACHKW0_003269"
     CDS             13509..14399
                     /gene="alsR"
                     /locus_tag="ACHKW0_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose utilization transcriptional regulator
                     AlsR"
                     /protein_id="HFO7476057.1"
                     /translation="MSQSEFDSALPNGIGLAPYLRMKQEGMTENESRIVEWLLKPGNL
                     SCAPAIKDVAEALSVSEAMIVKVSKLLGFSGFRNLRSALEDYFSQSEQVLPSELAFDE
                     APQDVVNKVFNITLRTIMEGQSIVNVDEIHRAARFFYQARQRDLYGAGGSNAICADVQ
                     HKFLRIGVRCQAYPDAHIMMMSASLLQEGDVVLVVTHSGRTSDVKAAVELAKKNGAKI
                     ICITHSYHSPIAKLADYIICSPAPETPLLGRNASARILQLTLLDAFFVSVAQLNIEQA
                     NINMQKTGAIVDFFSPGALK"
     gene            14458..15393
                     /gene="alsB"
                     /locus_tag="ACHKW0_003270"
     CDS             14458..15393
                     /gene="alsB"
                     /locus_tag="ACHKW0_003270"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose transporter substrate-binding protein"
                     /protein_id="HFO7476058.1"
                     /translation="MNKYLKYFSGTLVGLMLSTSAFAAAEYAVVLKTLSNPFWVDMKK
                     GIEDEAKTLGVSVDIFASPSEGDFQSQLQLFEDLSNKNYKGIAFAPLSSVNLVMPVAR
                     AWKKGIYLVNLDEKIDMDNLKKAGGNVEAFVTTDNVAVGAKGASFIIDKLGAEGGEVA
                     IIEGKAGNASGEARRNGATEAFKKASQIKLVASQPADWDRIKALDVATNVLQRNPNIK
                     AIYCANDTMAMGVAQAVANAGKTGKILVVGTDGIPEARKMVEAGQMTATVAQNPADIG
                     ATGLKLMVNAEKSGKVIPLDKAPEFKLVDSILVTQ"
     gene            15520..17051
                     /gene="alsA"
                     /locus_tag="ACHKW0_003271"
                     /pseudo
     CDS             15520..17051
                     /gene="alsA"
                     /locus_tag="ACHKW0_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418511.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter ATP-binding protein
                     AlsA"
     gene            17030..18010
                     /gene="alsC"
                     /locus_tag="ACHKW0_003272"
     CDS             17030..18010
                     /gene="alsC"
                     /locus_tag="ACHKW0_003272"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allose ABC transporter permease"
                     /protein_id="HFO7476059.1"
                     /translation="MGFTTRVKSEASEKKPFNFALFWDKYGTFFILAIIVAIFGSLSP
                     EYFLTTNNITQIFVQSSVTVLIGMGEFFAILVAGIDLSVGAILALSGMVTAKLMLAGV
                     DPFLAAMIGGVLVGGALGAINGCLVNWTGLHPFIITLGTNAIFRGITLVISDANSVYG
                     FSFDFVNFFAASVIGIPVPVIFSLIVALILWFLTTRMRLGRNIYALGGNKNSAFYSGI
                     DVKFHILVVFIISGVCAGLAGVVSTARLGAAEPLAGMGFETYAIASAIIGGTSFFGGK
                     GRIFSVVIGGLIIGTINNGLNILQVQTYYQLVVMGGLIIAAVALDRLISK"
     gene            18021..18716
                     /gene="alsE"
                     /locus_tag="ACHKW0_003273"
     CDS             18021..18716
                     /gene="alsE"
                     /locus_tag="ACHKW0_003273"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-allulose-6-phosphate 3-epimerase"
                     /protein_id="HFO7476060.1"
                     /translation="MKISPSLMCMDLLKFKEQIEFIDSHADYFHIDIMDGHFVPNLTL
                     SPFFVSQVKKLATKPLDCHLMVTRPQDYIAQLARAGADFITLHPETINGQAFRLIDEI
                     RCHDMKVGLILNPETPVEAMKYYIHKADKITVMTVDPGFAGQPFIPEMLDKLAELKAW
                     REREGLEYEIEVDGSCNKATYEKLMAAGADVFIVGTSGLFNHAENIDEAWRIMTTQIL
                     AAKSEVQPHAKTA"
     gene            18700..19629
                     /gene="alsK"
                     /locus_tag="ACHKW0_003274"
     CDS             18700..19629
                     /gene="alsK"
                     /locus_tag="ACHKW0_003274"
                     /EC_number="2.7.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0019316 - D-allose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allose kinase"
                     /protein_id="HFO7476061.1"
                     /translation="MQKQHNVVAGVDMGATHIRFCLRTAEGETLHCEKKRTAEVIAPG
                     LVSGIGEMIDEQLRRFNARCHGLVMGFPALVSKDKRTIISTPNLPLTAADLYDLADKL
                     ENTLNCPVEFSRDVNLQLSWDVVENRLTQQLVLAAYLGTGMGFAVWMNGAPWTGAHGV
                     AGELGHIPLGDMTQHCACGNPGCLETNCSGMALRRWYEQQPRNYPLSDLFVHAENAPF
                     VQSLLENAARAIATSINLFDPDAVILGGGVMDMPAFPRETLIAMTQKYLRRPLPHQVV
                     RFIAASSSDFNGAQGAAILAHQHFLPQSCAKAP"
BASE COUNT         4592 a         5490 c         5334 g         4255 t
ORIGIN      
        1 ataacgcctt ctgatacgtt taaaacgtca gaaagataaa aatatcatgt gaataaaaaa
       61 aagaacaagt agagcattaa cattatctta aataataaat agaggcaaaa agattatttt
      121 ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta aaatcagcag tacccccgac
      181 aaactcaggg attttgtgta taattgcggc ctttttcggc aatctgccgt tttttggcgc
      241 ttttgccctg ctgacttttg aggaaatcca catgtcatta ccacactgcc caaaatgcaa
      301 ctccgaatac acttacgaag ataacggcat gtacatctgc ccggaatgtg cctacgaatg
      361 gaacgacgca gaacctgcac aggaaagcga cgagctgatc gttaaagatg ctaacggcaa
      421 tctgctggct gacggcgaca gcgttaccat cattaaagat ctgaaggtga aaggtagctc
      481 ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc ctggttgaag gcgaccataa
      541 catcgattgc aaaatcgacg gttttggtcc gatgaaactg aaatctgagt ttgtgaaaaa
      601 gaactgattg tattgtgatc ggtaagccgg ataaggcgct cgcgccgcat ccggcaacga
      661 tgccgactgc ctgatgcgac gctgaccgcg tcttatcagg cttctctctc atctgtataa
      721 atttcgaact acacttaact ggcttctctt aactgaggtc accatcatgc cgttaagtcc
      781 ctacctctct tttgccggta actgtgccga cgcaattgct tattatcaac gtacgttggg
      841 cgctgaactg ctctataaaa tcagcttcgg cgaaatgcca aaatcagcgc aggacagcgc
      901 cgagaactgc ccttccggaa tgcaatttcc cgataccgcc atcgcccacg ccaacgtgcg
      961 cattgccgga agcgacatca tgatgagcga tgctataccg tcaggaaaag ccagctactc
     1021 cggctttacg ctggtgctcg attcgcaaca ggtcgaagaa ggaaaacgct ggtttgacaa
     1081 tcttgccgct aacggaaaaa tcgaaatggc ctggcaggaa actttctggg cgcatggctt
     1141 tggcaaagtc accgataaat ttggcgtacc gtggatgatt aatgtcgtca aacaacaacc
     1201 aacgcaataa cccgccggga ggcccgccct cccgcactgt catcgaattc ccgttaactc
     1261 ttcatctgtt agtcactttt aattaaccaa atcgtcacaa taatccgcca cgatggagcc
     1321 acttttttag ggaggctgca tcatgcaaac gattatccgt gtcgagaagc tcgccaaaac
     1381 cttcaatcag catcaggcgc tgcatgcggt tgatctgaac attcatcacg gtgaaatggt
     1441 ggctctgctt gggccgtcgg gttccggcaa atccaccctt ttacgtcact taagcggttt
     1501 gattaccggc gataaatccg ccggcagcca tatcgagctg ctgggccgca cagtccagcg
     1561 cgaaggccgt ctggcgcgcg atatccgcaa aagccgcgcc aacaccggct acatcttcca
     1621 acaattcaac ctggtgaacc gcctgagcgt actggagaac gtgctgattg gcgcgctcgg
     1681 cagcacgccg ttctggcgca cctgttttag ctggttcacc ggcgagcaga aacagcgcgc
     1741 gttacaggcg ctgacccgcg ttggcatggt gcattttgcc catcagcgcg tttccaccct
     1801 ctccggcgga cagcagcagc gtgtggcgat tgcccgcgcg ctgatgcagc aggcgaaggt
     1861 gattctggcc gatgaaccca tcgcctcgct ggacccggaa tccgcccgca tcgtgatgga
     1921 caccctgcgc gacatcaatc agaacgacgg catcaccgtg gtcgtcacgc tgcatcaggt
     1981 ggattacgcc ctgcgctact gcgaacgcat cgtcgccctg cgccaggggc acgttttcta
     2041 cgacggcagc agccaacagt ttgataacga acgttttgac catctctacc gcagcattaa
     2101 tcgcatcgaa gagaacgcga aagctgcctg acatccccat cattgaggaa aacgaatgaa
     2161 cgctaagata attgcctcgc tggccttcac cagcatgttc agcctcagca ccctgttaag
     2221 cccggcacac gccgaagagc aggaaaaggc gctgaatttc ggcattattt caacggaatc
     2281 acagcaaaac ctgaaaccgc aatggacgcc attcttacag gatatggaga agaagctggg
     2341 cgtgaaggtg aacgccttct ttgccccaga ctacgcaggc attatccagg gaatgcgctt
     2401 caataaagtg gatatcgcct ggtacggcaa cctgtcggca atggaagcgg tggatcgcgc
     2461 caacggccag gtcttcgccc agacggtcgc ggcggatgga tcgccaggtt actggagcgt
     2521 gttgatcgtc aacaaagata gtccgatcaa caacctgaac gatctgctgg cgaagcggaa
     2581 agatctcacc ttcggcaatg gcgatcctaa ctccacctct ggcttcctcg tccccggtta
     2641 ctacgtcttt gccaaaaaca atatctccgc cagcgacttt aagcgcaccg tcaacgctgg
     2701 gcatgaaacc aacgcgctgg ccgtcgccaa caagcaggtg gatgttgcca ccaacaacac
     2761 cgaaaacctc gacaagctga aaacctccgc gccagagaag ctgaaagaac tgaaggtgat
     2821 ctggaagtcg ccgctgatcc caggcgatcc gatcgtctgg cgtaaaaacc tttccgaaac
     2881 caccaaagac aagatctacg acttctttat gaactacggc aaaacgccgg aagaaaaagc
     2941 ggtgctggaa cgcctgggct gggcgccgtt ccgcgcttcc agcgacctgc aactggtgcc
     3001 gattcgccag ctcgcgctgt ttaaagagat gcagggcgtg aaaagcaata aaggactgaa
     3061 tgagcaggac aagctggcaa aaaccaccga gattcaggcg cagctggatg acctggaccg
     3121 cctgaacaac gcgttaagcg cgatgagttc ggtgagtaaa gcggtgcagt aaatcgtagg
     3181 tcggataaga cgccccggcg tcgcatccga caatgtgcag gcgttgatgc cggatgcggt
     3241 gcaagcacct tatccggcct acagaccgga gccaaacatg caaaccatca ccatcgcccc
     3301 acccaagcgc agctggttct cgcttctgag ctgggccgtt gttctcgccg tgttggtcgt
     3361 ctcgtggcag ggcgcggaaa tggccccgct cacgctgatt aaagacggcg gcaacatggc
     3421 gacgttcgcc gccgacttct tcccgcccga tttcagccag tggcaggatt acctcaccga
     3481 aatggccgtc acgctgcaaa tcgccgtctg gggcacggcg ctggcggtgg ttctctccat
     3541 ccccttcggc ctgatgagcg ccgaaaacct ggtgccgtgg tgggtttacc agcccgttcg
     3601 ccgcctgatg gacgcctgcc gcgccattaa cgaaatggtc ttcgccatgc tgttcgtggt
     3661 cgccgtcggc ctcggcccgt tcgctggcgt gctggcgctg tttatccaca ccaccggcgt
     3721 gctctccaaa ctgctttccg aagcggtgga agcgattgaa cccggcccgg tggaaggcat
     3781 tcgcgccacc ggcgccaaca agctcgaaga gatcctctac ggcgtgctgc cgcaggtgat
     3841 gccgctgctg atctcctact ccctctatcg cttcgaatcc aacgtccgct cggcgaccgt
     3901 cgtcggcatg gtcggtgcag gcgggatcgg cgtcaccctg tgggaagcga ttcgcggttt
     3961 ccagttccaa caaacctgcg ccctgatggt gcttatcatc gtcacggtca gcctgctgga
     4021 tttcctctct caacggttgc gtaagcactt tatctgataa gcgaggcatt gatatctatg
     4081 cacttgtcta cacatccgac cagctaccca acacgctatc aggagatagc cgcaaaactt
     4141 gagcaggagc ttcgtcaaca ctaccgctgc ggcgactatc ttcccgccga gcagcaactg
     4201 gcggcgcgct ttgaggtgaa ccgccacacc ctgcgccgcg ccatcgacca actggtggaa
     4261 aaaggctggg tacagcgccg tcagggcgtc ggcgtgctgg tgctgatgcg cccgttcgat
     4321 tacccgctca acgcccaggc gcgttttagc cagaacctgc tggaccaggg tagccatccc
     4381 accagcgaaa aactgctgtc ggtattgcgc cccgcgtccg gtcacgtcgc tgacgcgctg
     4441 gggattaccg agggcgagaa cgttatccat ttgcgcaccc tgcgtcgcgt caacggcgtc
     4501 gcgctctgtt taatcgacca ctactttgcg gacctcaccc tttggccgac gctgcaacgc
     4561 ttcgacagcg gttcgctgca cgattttctg cgcgagcaaa ccggaattgc gctgcgccgc
     4621 agccagacgc ggatcagcgc ccgtcgcgcc caggccaaag agtgccagcg tcttgaaatt
     4681 ccgaatatgt cgccgctgct gtgcgtgcgc acccttaacc accgtgacgg tgaaagcagc
     4741 ccggcggagt actccgtcag cctgacgcgc gccgacatga ttgaattcac tatggagcac
     4801 tgaatgcacg cagataccgc gacccgccag cactggatgt ccgtactggc gcacagccaa
     4861 ccggctgaac tggcagcccg cctgaaagca ctgaacatca ccgccgacta tgaggttatc
     4921 cgcgccgccg aaaccggcct ggtgcagatt caggcgcgga tgggcggcac cggcgaacgc
     4981 ttttttgccg gcgacgccac gctcacccgc gccgccgtac gcctgactga cggcacgctc
     5041 ggctacagct gggtgctggg gcgcgacaaa cagcacgccg aacgctgcgc gctgattgac
     5101 gcgctgatgc agcaatctcg ctattttcaa aacttatcag aaacccttat tgccccgctg
     5161 gacgctgacc gtatggcacg cattgccgca cgccaggccg aagtgaacgc cagccgggtc
     5221 gacttcttta cgatggttcg cggagacaac gcatgaccct ggaaaccgct tttatgcttc
     5281 ccgtgcagga tgcccagcac agttttcgtc gcctgttaaa ggccatgagc gagccgggcg
     5341 tgattgtcgc cctgcatcag ctcaagcgcg gctggcagcc gctgaatatc gccaccacca
     5401 gcgtgctgct gacgctggcc gataacgaca cgccggtgtg gcttgccgcg ccattgagta
     5461 acgatatcgt cagccagagc ctgcgttttc ataccaacgc gccgctggtc agccagccgg
     5521 aacaggcgac cttcgcggtg acggatgagg cgatttccag cgaacagctt aacgcccttt
     5581 ccaccggcac cgccgttgcg ccggaagcgg gtgcaacgct gattttacag gtcgccagcc
     5641 tgagcggcgg gcgcatgtta cgtctcaccg gcgcgggcat tgccgaagaa cgaatgatcg
     5701 ctccgcagct gccggagtgc attctgcacg aactcaccga gcgcccgcac ccgttcccgc
     5761 tcggcatcga cctgatcctg acctgcggcg agcgcctgct ggctattccg cgaaccactc
     5821 atgtggaggt gtgctgatgt acgttgccgt gaaagggggc gagaaggcga tcgacgccgc
     5881 tcacgccctg caagagagcc gacgccgggg cgataccgat ttgcctgaac tgagcgtcgc
     5941 ccagattgaa cagcagctta atcttgcggt ggatcgcgtg atgaccgaag gcggcattgc
     6001 cgaccgcgaa ctggcggcgc tggcgctgaa acaggccagc ggcgataacg tcgaagcaat
     6061 tttcctgctg cgcgcctacc gcaccacgct ggcgaagctc gcggtaagcg agccgctcga
     6121 caccacagag atgcgtctcg aacggcgtat ttcggcggtt tataaagaca ttcccggcgg
     6181 ccagctgctt ggcccaacct acgactacac ccatcgcctg ctcgatttca ccctgctggc
     6241 aaacggtgaa gcgccgacgc tgaccaccgc cgacagcgaa caacagccgt cgccgcacgt
     6301 tttcagcctg ctggcgcgtc aggggctggc gaagtttgaa gaggatagcg gcgcacagcc
     6361 ggatgatatc acccgcacgc cgccggttta cccctgctca cgttcttccc gtttgcagca
     6421 gttgatgcgc ggcgacgaag gctatttgct ggcgctggcc tactccaccc aacgcggtta
     6481 cgggcgcaat cacccgttcg cgggcgagat ccgcagtggt tacatcgacg tgtcgattgt
     6541 gccggaagag ctgggatttg cggtgaacgt cggcgaactg ctgatgaccg aatgtgaaat
     6601 ggtcaacggt tttatcgacc cgccggatga gccgccgcac ttcacgcgcg gctacgggct
     6661 ggtattcggc atgagcgagc gcaaagcgat ggcaatggcg ctggttgacc gtgcgttgca
     6721 agccccggaa tacggcgagc acgcgacagg cccggcgcag gatgaagagt tcgtgctggc
     6781 gcatgccgac aacgtcgaag ccgcaggctt tgtctcgcac ctcaaactcc cgcactacgt
     6841 cgatttccag gccgaactgg agctactcaa acgtctgcaa caggagcaga accatggcta
     6901 atctgagcgg ctacaacttt gcctacctcg acgagcagac caaacgcatg atccgccgcg
     6961 ccatcttaaa agcggtggct atccccggtt atcaggtgcc gtttggcggg cgcgagatgc
     7021 cgatgcccta cggctgggga accggcggca ttcagctcac cgccagcgtg attggcgaaa
     7081 gcgacgtgtt gaaggtgatt gaccagggtg cggatgacac caccaacgcc gtgtcgattc
     7141 gcaacttctt taagcgcgtg accggggtaa acaccactga acgtacggac gatgcgacgc
     7201 ttatccagac gcgtcaccgc atccccgaaa cgccgctgac cgaagatcag atcattatct
     7261 tccaggtgcc aatcccggaa ccgctgcgct ttatcgagcc gcgcgaaacg gagacccgca
     7321 ctatgcacgc gctggaggag tacggcgtga tgcaggtgaa actgtatgaa gatatcgccc
     7381 gcttcggtca tatcgccacc acctatgctt atccggtgaa ggtgaacggg cgctacgtga
     7441 tggacccgtc gccgatcccg aaattcgata acccgaaaat ggacatgatg cccgccctgc
     7501 agctgttcgg agcagggcgc gagaagcgca tctatgcggt gccgccgttt acccgcgtgg
     7561 aaagtctcga tttcgacgat cacccgttca ccgttcagca gtgggatgag ccatgcgcca
     7621 tctgcggatc gacccacagc tatcttgatg aagtggtgct ggatgacgcc ggaaaccgca
     7681 tgtttgtctg ctccgatacc gattattgcc gccaacagag cgaggcgaaa agccaatgaa
     7741 taaaccgtta ctttcggtca ataacctgac ccacctttac gcgccgggca aaggctttag
     7801 cgatgtctct tttgatttat ggccgggaga agtgctgggc attgtcggag aatccggctc
     7861 cgggaagacc acgctgctga agtcgatctc cgcgcgtctg acgccgcagc agggggagat
     7921 tcactacgag aaccgttcgc tgtatgcaat gagcgaggcc gaccgccgtc gcctgctgcg
     7981 caccgaatgg ggcgtagtgc atcagcatcc actcgacggc ctgcgccgtc aggtctcggc
     8041 aggcggcaat atcggcgagc ggttgatggc gaccggggcg cgtcattacg gcgacattcg
     8101 cgccaccgcg cagaagtggc tggaagaggt ggagattccc gccaaccgga tcgacgacct
     8161 gccgaccacc ttttccggcg gtatgcagca gcgtttgcag attgcccgca acctggtgac
     8221 gcatccgaag ctggtgttta tggatgaacc gaccggcggg ctggatgtgt cggtgcaggc
     8281 ccgcctgctc gacctgctgc gcggcctggt ggtggagctg aacctcgcgg tggtgattgt
     8341 cacccatgat ttaggcgtcg cccgcctgct ggcggaccgt ttgctggtga tgaagcaggg
     8401 gcaagtggtg gagagtgggt taaccgaccg cgtgctcgac gacccgcatc atccgtatac
     8461 acagctgctg gtgtcatcgg ttttgcagaa ttgagccggt gccggatgcg gcgtaaacgc
     8521 cttatccggc ctacaaatgc gctccccgta ggtcggataa gacgcgtcag cgtcgcatcc
     8581 gacacccgaa ccacgaggcg aaaaatgatt aacgtacaaa acgtcagtaa aaccttcatc
     8641 ctgcaccagc aaaacggcgt gcgcctgccc gtcctcaatc gcgcctcgct caccgtcaac
     8701 gcgggcgaat gcgtggtgct ccacggccat tccggcagcg gcaaatcaac tctgctacgc
     8761 tcgctgtacg ccaactatct acccgacgaa ggtcaaatcc agatcaaaca cggtgacgag
     8821 tgggtagacc tggtcaccgc gccagcgcgc aaagtggtgg aaatccgcaa aaccaccgtc
     8881 ggctgggtga gccagtttct gcgcgtcatc ccgcgtatct cagcactgga agtggtgatg
     8941 cagccgctgc tcgataccgg cgttccgcgt gaagcctgcg ccgctaaagc cgcgcgtctt
     9001 ctcacccgcc tgaacgtgcc ggaacgcctg tggcacctgg caccatcgac attttccggt
     9061 ggcgaacagc agcgcgtcaa catcgcccgc ggctttatcg tcgactaccc cattctgctg
     9121 cttgacgaac ctaccgcctc gctggacgcc aaaaacagcg ccgcggtggt ggaactgatt
     9181 cgcgaagcca aaacccgtgg cgcagccatc gtaggcatct tccatgacga agctgtacgt
     9241 aatgacgtcg ccgaccgcct gcacccaatg ggagcctctt catgattatc aataacgtta
     9301 agctggtgct ggaaaacgag gtggtgcacg gttcgctgga ggtgcaggat ggcgaaatcc
     9361 gcgcctttgc cgaaagccag agccgcctgc cagaagcgat ggacggcgaa ggtggctggc
     9421 tactacccgg cctgattgag ctgcataccg acaacctcga taaattcttc accccgcgcc
     9481 cgaaagtcga ctggcccgcc cattcggcga tgagcagcca cgacgcgctg atggtggcga
     9541 gcggcatcac caccgtactg gatgccgtag caattggcga cgtgcgcgac ggcggcgatc
     9601 ggctggaaaa tctggagaag atgatcaacg ccatcgaaga gacgcagaaa cgcggcgtca
     9661 accgcgccga gcaccgcctg catctgcgct gcgaactgcc gcatcacact acgctgccgc
     9721 tgtttgaaaa actggtgcag cgcgagccgg tgacgctggt gtcgctgatg gatcactcgc
     9781 cgggccagcg ccagttcgcc aaccgcgaga agtatcgcga gtattatcag ggcaaatact
     9841 cgctcaccga tgcgcagatg cagcagtacg aagaagagca actggcgctc gccgcacgct
     9901 ggtcgcagcc gaatcgcgaa actatcgccg ccctgtgccg cgtaagacaa attgccctcg
     9961 ccagccacga tgacgccacg cacgcccacg ttgccgaatc ccaccagctt ggcagcgtga
    10021 tcgccgaatt tcccactacg ttcgaagcag cggaagcctc gcgcaagcat ggcatgaacg
    10081 tgctgatggg cgcgccaaat atcgtgcgtg gcggttcgca ctccggcaac gtggcggcca
    10141 gtgaactggc gcagcttggc ctgctggata tcctctcttc cgactactac cccgccagcc
    10201 tgctggatgc ggcgttccgc gtcgccgatg acgagagcaa ccgctttacg ctgccgcagg
    10261 cggtgaagct ggtgaccaaa aatccggcgc aagcgcttaa tctccaggat cgcggggtga
    10321 ttggcgaggg taaacgcgcc gacctggtgc tggcgcatcg caagggcaac cacattcata
    10381 tcgaccacgt ctggcgtcag ggtaaaaggg tgttctgatg atgggaaaac tgatttggtt
    10441 aatggggccg tccggctccg ggaaagatag cctgctggcg gaactccgcc tgcgggaaca
    10501 aactcagcta ctggtggcgc atcgctacat cacgcgcgac gccagcgctg gaagcgaaaa
    10561 ccatatcgcc ctgagcgagc aggagttttt tacccgcgcg gggcaaaatc tgctggcctt
    10621 aagctggcac gctaacggtc tgtattatgg cgtcggcgtc gagattgacc tctggctgca
    10681 cgctggattc gacgtgctgg tcaacggctc acgcgcccat ctgccgcagg cgcgggcgcg
    10741 ctatcaatcg gcgctgctgc ccatttgctt acaggtttcg ccagagatcc tgcgccagcg
    10801 cctggaaaac cgtggccgcg aaaacgccag tgaaattaac gcccggctgg cgcgcgccgc
    10861 ccgctatact ccacaggatt gccatacgct caataatgac ggcagcctgc gccagtcggt
    10921 cgacacgctg ctgacgctaa tccatcagaa ggagaaacac catgcctgct tgtgagcttc
    10981 gcccggccac gcagtacgac accgacgcgg tttacgcgtt gatttgcgag ctaaaacagg
    11041 cggagtttga ccaccacgcg tttcgcgtgg gctttaacgc caacctgcgc gacccgaaca
    11101 tgcgctacca tctggcgctg ctggatggcg aagttgtcgg tatgatcggc ctgcatttgc
    11161 agtttcatct gcatcatgtc aactggatcg gcgaaattca ggagttggtg gtaatgccgc
    11221 aggcgcgcgg tctgaacgtc ggcagtaagt tactggcgtg ggcagaagaa gaagcccgcc
    11281 aggccggggc cgaaatgacc gaactttcga ccaacgtgaa gcgccacgac gcgcaccgtt
    11341 tctatctgcg agaaggctac gagcagagtc acttccgctt caccaaggcg ctgtaacatg
    11401 agtctgaccc tcacgctcac cggcaccggc ggcgcacagg gcgttccggc atggggctgc
    11461 gagtgcgcgg cctgcgaccg agcgcggcgc tcaccgcagt atcgccgcca gccatgcagc
    11521 ggcgtagtga agtttaacga cgcaatcacc ctgatcgacg ccgggctgca cgatctcgcc
    11581 gatcgctggt cgcccggatc gttccagcag tttttgctga cgcattatca tatggatcac
    11641 gtccaggggc tgtttccgct gcgctggggc gttggcgatc cgatcccggt ttacggcccg
    11701 ccggatgacc agggttgcga cgatctgttt aaacatccgg gcctgcttga tttcagccac
    11761 acagtggaac cgtttgtggt atttgatttg caggggttgc aggtcacgcc gctgccgctt
    11821 aaccactcaa aactgacctt cggttatctg ctggaaacgg cacatagccg ggtggcgtgg
    11881 ctatctgaca ccgctggcct gccggaaaaa acacagaaat ttttactcaa taatcatccg
    11941 caggtgatgg tgattgattg cagccacccg ccgcgcgagg atgcgccgcg taatcactgt
    12001 gatttaaata ccgtgcttgc gctcaatcag gttatccact cgccacgggt gattctgacc
    12061 catatcagcc accagtttga tgcgtggctg atggagaatg cgctgccgcc gggttttgag
    12121 gcggggtttg atgggatgga gattggggtg gcgtgatgag agggaatgtg cgcgctggcc
    12181 ccctcaccct aaccctctcc ccagaggggc gaggggaccg attgtgctcg atattgaata
    12241 ttgcgctggt tttctccctc tccccattgg ggtgaggggc gatgcctgct ccatacccaa
    12301 cctcatcgcc catactcatc ttccattctc cgctcttcat cttctagctg ccgacgctcc
    12361 tgatcaagct ggcgctggcg atcgtccagc tgcctgcggc gatcttcaaa ctggcggcgg
    12421 cggtcgtcat attgtctgcg ccgatcgtcg ctcacttcac gctgccagcc gtcgtcgcgc
    12481 gaatcttcat agtctcgccc acggtcaggg ttataagcgt cgttaatcgc ctgctgaata
    12541 ttgccaatgg tgtcgtcgat aatatcggcc tgggccggaa cggtggacag cgtgagcagg
    12601 gtgaataaaa gaaatagcgg aaagcgtttc attagccaac ctcaaaaaga aactctatcc
    12661 acattagtca ttactcatcc atgcaagtag tggatgaatc tcaatttctc cactgctcta
    12721 ttgccgtaat cgcctccacg cgttgttgat gacgaccgcc ttcgtactgt gcgcccagcc
    12781 acgcatccac aatcattttt gccagttcga ggccaaccac tcgtgaacca aaagccagca
    12841 cgttggtgtc gttatgctgc cgcgaaagtt gcgcggaata aggttcgcta cagacgaccg
    12901 cgcgaattcc ggcaaacttg ttcgccgcta tcgaaatacc gacgccagta ccacaaatca
    12961 aaatcccgcc atcaacctct ccgccagcaa cagccagtgc gacttgactg gcgtaatgtg
    13021 gataatcagt acgctctgac gaccaggttc ctttatcaat cacttcaacg ccacgctcaa
    13081 ctaaatgtgc cactatttca tgttttaaaa tgaaaccgac atgatcacag ccaaatgcaa
    13141 tctttttcat tcatcttccc cgtccgtgca aaacttcaca atcaaaaaac atttgtgatg
    13201 ttaatgaatt aaaaacaccc aaaatcgatg aattacgcct tcctgatctg ttttacagag
    13261 ttacatctga cagcgcaata gcgttaaaga cactcaccat ccagcaacat ctatcatcta
    13321 aaaaaccaga aaaacaaata acatcatgtt tttaaactaa ttaaatgaaa taaaatttta
    13381 agccactcgc cattgttcac aataaaataa actttataaa ttttattttt ttgtgaagtc
    13441 gccagcatct tttctgttct tgctgtggtg atatagtggc gtcttcaatt caaggacaag
    13501 agaacgtgat gagccagtca gagtttgatt cagcgcttcc gaacggtata gggttagcgc
    13561 cttacctgcg aatgaagcag gaaggaatga cagaaaatga aagccgcatc gtagagtggt
    13621 tactcaaacc cggtaacctg agttgtgcac ccgcaattaa agatgtcgca gaagctctgt
    13681 cggtatctga agcgatgata gttaaggtat caaagctgct ggggtttagc ggctttcgta
    13741 acttacgcag tgcgctggaa gattattttt ctcagtcaga acaggtattg ccttccgagt
    13801 tggcttttga tgaagcgccg caggatgtgg tgaataaggt atttaacatc actttacgca
    13861 ccattatgga aggtcagtcg atcgtcaacg ttgatgagat ccaccgtgcc gcccgctttt
    13921 tctatcaggc cagacagcgg gatttgtacg gtgccggagg atcaaatgct atctgtgctg
    13981 atgtacagca caagttcttg cgcattggcg tacgctgtca ggcctatcct gatgctcaca
    14041 tcatgatgat gtccgcttcg ttgttacagg aaggagatgt tgtgctggta gtgacccatt
    14101 ccgggcgaac cagtgatgta aaagcggccg tagaactggc aaaaaagaac ggggcaaaga
    14161 ttatttgtat aacccatagc taccattcac cgatagcgaa actggccgat tatattattt
    14221 gctcaccagc cccggaaacg ccgttattag gtcgtaatgc ctcggcaaga atattacaac
    14281 taactttgct ggacgctttt tttgtctctg tcgcccagct caacattgaa caagctaata
    14341 ttaatatgca aaaaaccggc gcaattgttg atttcttctc accaggcgcg ctgaaataag
    14401 aaaaaggtaa ttccgccatc gcggagttac ctgtcccctt accgtgagaa cattattatg
    14461 aataaatatc tgaaatattt cagcggcaca ctcgtgggct taatgttgtc aaccagcgct
    14521 tttgctgccg ccgaatatgc tgtcgtattg aaaaccctct ccaacccatt ttgggtagat
    14581 atgaaaaaag gcattgaaga tgaagcaaaa acactgggcg tcagcgttga tatttttgcc
    14641 tctccttcag aaggcgattt tcaatctcaa ttgcagttat ttgaagatct cagtaataaa
    14701 aattacaaag gtatcgcctt cgctccatta tcctcagtga atctggtcat gcctgtcgcc
    14761 cgcgcatgga aaaaaggcat ttatctggtt aatctcgatg aaaaaatcga catggataat
    14821 ctgaaaaaag ctggcggcaa tgtggaagct tttgtcacca ccgataacgt tgctgtcggg
    14881 gcgaaaggcg cgtcgttcat tattgacaaa ttgggcgctg aaggtggtga agtcgcaatc
    14941 attgagggta aagccggcaa cgcctccggt gaagcgcgtc gtaatggtgc caccgaagcc
    15001 ttcaaaaaag caagccagat caagcttgtc gccagccagc ccgctgactg ggaccgcatt
    15061 aaagcactgg atgtcgccac taacgtgttg caacgtaatc cgaatattaa agcgatctat
    15121 tgcgcgaatg acacgatggc aatgggtgtt gctcaggcag tcgcaaacgc cggaaaaacg
    15181 ggcaaaatat tggtcgtcgg tacagatgga attccggaag cccgcaaaat ggtggaagcc
    15241 ggacaaatga ccgcgacggt tgcgcagaac ccggcagata ttggcgcaac gggtctgaag
    15301 ctgatggtta acgctgagaa atccggcaag gttatcccgc tggataaagc accggaattt
    15361 aaactggtcg attcaatcct ggtcactcaa taaaactgaa tgatgccgga tgatgatgct
    15421 aatgaagtgt cttatccggc caacaaaata acctgaacgt agacctgata aatgtagcca
    15481 tcaggcaata atacgatatt tcgtgcagag aggttattta tggccacgcc atatatatcg
    15541 atggcgggga tcggcaagtc ctttggtccg gttcacgcat taaagtcggt taatttaacg
    15601 gtttatcctg gtgaaataca tgcattacta ggagaaaatg gcgcgggtaa atccacgcta
    15661 atgaaggttt tatccggaat acatgagccg accaaaggca ccattaccat taataacatt
    15721 agctataaca agctggatca taaattagcg gcacaactcg gtatcgggat tatttatcag
    15781 gaactcagcg ttattgatga attaaccgta ctggaaaatt tatatattgg tcgtcatctg
    15841 acgaaaaaaa tctgtggcgt caatattatc gactggcgag aaatgcgtgt ccgcgccgcc
    15901 atgatgttat tacgcgtggg cttgaaagtt gatttagatg agaaagtggc gaatctatct
    15961 atcagccaca agcagatgct ggaaattgcc aaaacgctga tgctcgatgc caaagtcatc
    16021 atcatggatg aacccacctc ctcactcacc aataaagagg tggactatct gtttctgatc
    16081 atgaatcagt tgcgtaaaga gggtacggcc atcgtctata tctcgcataa gttggcggaa
    16141 attcgccgta tttgcgaccg ctatacggtg atgaaagacg gcagcagcgt ttgcagcggc
    16201 atggtaagcg atgtgtcaaa tgacgatatc gtccgtctga tggtaggccg cgaactgcaa
    16261 aaccgtttta acgcgatgaa ggagaatgtc agcaaccttg tgcacgatac ggtttttgag
    16321 gtgcgcaacg tcaccagccg tgacagaaaa aaggtccggg atatctcatt tagcgtctgc
    16381 cggggagaat attaggcttt gccgggctgg tcggttccgg acgtactgaa ctgatgaatt
    16441 gtctgtttgg cgttgataaa cgcgctggcg gagaaatccg tcttaatggc aaagatatct
    16501 ctccacgttc ccccctggat gcagtgaaaa aagggatggc ttacatcact gaaagccgcc
    16561 gggataacgg tttttttccc aacttttcca tcgctcagaa catggcgatc agccgcagtc
    16621 tgaaagacgg cggctataaa ggcgcgatgg gcttgtttca tgaagttgac gagcaacgta
    16681 ccgctgaaaa tcaacgcgaa ctgctggcgc tgaaatgtca ttcggtaaac cagaatatca
    16741 ccgaactatc cgggggaaat cagcagaaag tcctgatctc caaatggctg tgctgttgcc
    16801 cggaagtgat tattttcgat gaacctaccc gcggcatcga cgttggcgcg aaagccgaaa
    16861 tttacaaagt gatgcgccaa ctggcggacg acggaaaagt catcctgatg gtgtcatctg
    16921 aactacctga aattatcacc gtctgcgacc gcatcgccgt gttctgcgaa ggacgactga
    16981 cgcaaatcct gacgaatcgc gatgacatga gcgaagagga gattatggca tgggctttac
    17041 cacaagagta aaaagcgaag cgagcgagaa gaaaccgttc aactttgcgc tgttctggga
    17101 taaatacggc acctttttta tcctggcgat catcgtcgcc atttttggtt cgctgtcgcc
    17161 agaatatttt ctgaccacca ataatattac ccagattttt gttcaaagct ccgtgacggt
    17221 attgatcggc atgggcgagt ttttcgctat cctggtcgct ggtatcgacc tctcggttgg
    17281 cgcgattctg gcgctttccg gtatggtgac cgccaaactg atgttggcag gtgttgaccc
    17341 gtttctcgca gcgatgattg gcggtgtact ggttggcggc gcactggggg cgatcaacgg
    17401 ctgcctggtc aactggacgg ggctacaccc gttcatcatc acccttggca ccaacgcgat
    17461 tttccgtggg atcacgctgg tgatctccga tgccaactcg gtatacggct tctcctttga
    17521 cttcgtgaac ttctttgccg ccagcgtaat tgggatacct gtccccgtta tcttctcgct
    17581 aattgtcgcg ctcatccttt ggtttctgac aacgcgtatg cggctcgggc gcaacatcta
    17641 cgcactgggc ggcaacaaaa attcggcgtt ctattccggg attgacgtga aattccacat
    17701 cctggtggtg tttatcatct ctggtgtttg tgcaggtctg gcaggcgtcg tctcaactgc
    17761 acgactcggt gccgcagaac cgcttgccgg tatgggtttt gaaacctatg ccattgccag
    17821 cgccatcatt ggcggcacca gtttcttcgg cggcaagggg cgcattttct ctgtggtgat
    17881 tggcgggttg atcatcggca ccatcaacaa cggtctgaat attttgcagg tacaaaccta
    17941 ttaccaactg gtggtgatgg gcggattaat tatcgcggct gtcgcccttg accgtcttat
    18001 cagtaagtaa ggaattgaac atgaaaatct ccccctcgtt aatgtgtatg gatctgctga
    18061 aatttaaaga acagatcgaa tttatcgaca gccatgccga ttactttcac atcgatatca
    18121 tggatggtca ctttgtcccc aatctgacac tctcaccgtt cttcgtaagt caggttaaaa
    18181 aactggcaac taaaccgctc gactgtcatc tgatggtgac gcggccgcag gattacattg
    18241 ctcaactggc gcgtgcggga gctgatttca tcactttgca tccggaaact atcaacggtc
    18301 aggcgttccg cctgattgat gaaatccgct gtcatgacat gaaagtgggg ctgatcctta
    18361 acccggagac accagttgag gccatgaaat actatatcca taaggccgat aaaattacgg
    18421 tcatgactgt cgatcccggc tttgccggac aaccgttcat tcctgaaatg ctggataaac
    18481 ttgccgaact gaaggcatgg cgtgaacgag aaggtctgga gtacgaaatt gaggtggacg
    18541 gttcctgcaa caaggcaact tacgaaaaac tgatggcggc aggggcggat gtctttatcg
    18601 tcggcacttc cggcctgttt aatcatgcgg aaaatatcga cgaagcatgg agaattatga
    18661 ccacgcagat tctggctgca aaaagcgagg tacagcctca tgcaaaaaca gcataacgtc
    18721 gtagcgggcg tggatatggg ggcaacgcat atccgctttt gtctgcggac agcagaaggt
    18781 gaaacgctac actgcgaaaa aaagcggacc gcagaagtca ttgctcccgg cctggtgtcg
    18841 ggtatcggcg aaatgattga cgagcaactc aggcgcttta acgctcgctg tcatggtctg
    18901 gtgatgggat ttccggcgct ggtcagtaaa gataaacgca ccataatttc tacgcctaac
    18961 ctgccgttaa cagcggcgga tttatatgat ctcgccgata agctcgaaaa tacgctgaat
    19021 tgtccggttg agttttcccg cgacgttaac ctgcaactct cctgggacgt agtagaaaac
    19081 cgccttacgc aacaactggt tctggcggcc tatctcggta cggggatggg gttcgcagtg
    19141 tggatgaacg gtgcgccgtg gacgggtgca cacggtgtgg caggcgaact gggtcatatc
    19201 cccctgggag atatgaccca acactgcgcg tgtggcaatc ctgggtgcct ggaaaccaat
    19261 tgctctggaa tggcgctaag acgctggtac gaacaacagc cccgaaatta cccattgagc
    19321 gaccttttcg tccatgcgga aaacgcccct ttcgtccaga gtctgcttga aaacgcggca
    19381 cgggccattg ccaccagcat taatctgttc gatcccgatg cggtgattct gggcggtggc
    19441 gtgatggata tgcccgcctt cccacgcgag actctcattg ccatgaccca aaagtacctg
    19501 cgccgtccac tgccgcatca ggtcgtgcgc tttattgccg cctcatcttc tgactttaat
    19561 ggcgctcagg gtgcagcaat attggcgcat caacattttt tgccacagtc ctgtgctaaa
    19621 gccccatgag taaagcgttg gcacagtagc gcaatgtccc gctgtgccaa c
//
LOCUS       DAZFXT010000085        19483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.085, whole genome shotgun sequence.
ACCESSION   DAZFXT010000085 DAZFXT010000000
VERSION     DAZFXT010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.085"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..135
                     /locus_tag="ACHKW0_003275"
     CDS             <1..135
                     /locus_tag="ACHKW0_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO7476062.1"
                     /translation="RWHGSAEAHTCREVQDIPGVLAVFAERRKDSFGLYVRLMSVTLN
                     "
     gene            298..666
                     /locus_tag="ACHKW0_003276"
     CDS             298..666
                     /locus_tag="ACHKW0_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7476063.1"
                     /translation="MSDTLSGTTHPDDNHDHPWWGLPCTVTPCFGVCLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELSPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPEMKN"
     gene            756..1082
                     /locus_tag="ACHKW0_003277"
     CDS             756..1082
                     /locus_tag="ACHKW0_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7476064.1"
                     /translation="MKTLPDTHVREASRCPSPVIIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGFSACTHSQLINSIDILRARRATG
                     LMTRDN"
     gene            1130..1618
                     /locus_tag="ACHKW0_003278"
     CDS             1130..1618
                     /locus_tag="ACHKW0_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7476065.1"
                     /translation="MKLALTPEADSVNVQALNMGRIVVDVDGIELAELINVVCDNGHS
                     LRVVDESDRASTDCTPSFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRQGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPTF
                     DW"
     gene            1630..1827
                     /locus_tag="ACHKW0_003279"
     CDS             1630..1827
                     /locus_tag="ACHKW0_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7476066.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIHTLRQLQLLQQNR"
     gene            1912..2754
                     /locus_tag="ACHKW0_003280"
     CDS             1912..2754
                     /locus_tag="ACHKW0_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7476067.1"
                     /translation="MSDITISRPELVNRHTDVICSTSIRHILAVRKSALLQIDTLIRQ
                     LAEISAMTESIGGKTAPAWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDAQARDTWYQSLEYDNFPEISEANILSTFEQLHQNKDEVFERGMINVFRGLS
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIHLDDHIQSVQCKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPG
                     ALAM"
     gene            complement(3097..3633)
                     /gene="yghD"
                     /locus_tag="ACHKW0_003281"
     CDS             complement(3097..3633)
                     /gene="yghD"
                     /locus_tag="ACHKW0_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HFO7476068.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLKNAAPDIIAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIADRGENIQVWIDPVVFNDLLKWLNALDEKYALRVTQIDAS
                     AAEKPGMVNVQRLEFGRG"
     gene            complement(3635..4813)
                     /gene="gspL"
                     /locus_tag="ACHKW0_003282"
     CDS             complement(3635..4813)
                     /gene="gspL"
                     /locus_tag="ACHKW0_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7476069.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPDVARKQIPTILPALLEEELNQDIDELHFAPLNIDQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMACDAQRVICRIDECRGWS
                     AGLALAPVMFRAQLNEQDLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAVTTYGKP
                     EGNLLTGPWQPRVSYRKQWARWRVMILQILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQILHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(4810..5787)
                     /gene="gspK"
                     /locus_tag="ACHKW0_003283"
     CDS             complement(4810..5787)
                     /gene="gspK"
                     /locus_tag="ACHKW0_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7476070.1"
                     /translation="MITSPPKRGMALVVVLVLLAVMMLVTITLSGRMQQQLGRTRSQQ
                     EYQQALWYSASAESLALSALSLSLKNEKRVHLAQPWASGPRFFPLPQGQIAVTLRDAQ
                     ACFNLNALAQPTTASRPLAVQQLIALISRLDVPAYRAELIAESLWEFIDEDRSVQTRL
                     GREDSEYLARSVPFYAANQPLADISEMRVVQGMDAGLYQKLKPLVCALPMIRQQININ
                     TLDVTQSVILEALFDPWLSPVQARALLQQRPAKGWEDVDQFLAQPLLADVDERTKKQL
                     KTVLSVDSNYFWLRSDITVNETELTMNSLIVRMGPQHFSVLWHQTGESE"
     gene            complement(5790..6389)
                     /gene="gspJ"
                     /locus_tag="ACHKW0_003284"
     CDS             complement(5790..6389)
                     /gene="gspJ"
                     /locus_tag="ACHKW0_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001691118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7476071.1"
                     /translation="MRRTRAGFTLLEMLVAIAIFASLALMAQQVTNGVTRVNSAVAGH
                     DQKLNLMQQTMSFLTHDLTQMMPRPVRGDQGQREPALLAGAGVLASESEGMRFVRGGV
                     VNPLMRLPRSNLLTVGYRIHDGYLERLAWPLTDAAGSVKPTMQKLIPADSLRLQFYDG
                     TRWQESWSSVQAIPVAVRMTLHSPQWGEIERIWLLRGPQ"
     gene            complement(6386..6757)
                     /gene="gspI"
                     /locus_tag="ACHKW0_003285"
     CDS             complement(6386..6757)
                     /gene="gspI"
                     /locus_tag="ACHKW0_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001709020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7476072.1"
                     /translation="MKRGFTLLEVMLALAIFALAATAVLQIASGALSNQHVLEEKTVA
                     GWVAENQTALLYLMTREQRAVRHQGESDMAGSRWYWRTIPLNTGNALLQAVDIEVSRH
                     EDFSSVIQSRRAWFSAVGGQQ"
     gene            complement(6754..7317)
                     /gene="gspH"
                     /locus_tag="ACHKW0_003286"
     CDS             complement(6754..7317)
                     /gene="gspH"
                     /locus_tag="ACHKW0_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7476073.1"
                     /translation="MPERGFTLLEIMLVIFLIGLASAGVVQTFATDSESPAKKAAQDF
                     LTRFAQFKDRAVIEGKTLGVLIDPPGYQFMQRRQGQWSPVSSTRLSAQVTVPKQVQML
                     LQPGSDIWQKEYALELQRRRLTLHDIELELQKEAKKKTPQIRFSPFEPATPFTLRFYS
                     AAQNACWAVKLAHDGALSLNQCDERMP"
     gene            complement(7321..7776)
                     /gene="gspG"
                     /locus_tag="ACHKW0_003287"
     CDS             complement(7321..7776)
                     /gene="gspG"
                     /locus_tag="ACHKW0_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003912123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7476074.1"
                     /translation="MNSLSRTQKPRAGFTLLEVMVVIVILGVLASLVVPNLLGNKEKA
                     DRQKAISDIVALENALDMYRLDNGRYPTTEQGLEALIQQPANMADSRNYRTGGYIKRL
                     PKDPWGNDYQYLSPGEKGLFDVYTLGADGQENGEGAGADIGNWNLQEFQ"
     gene            complement(7793..9016)
                     /gene="gspF"
                     /locus_tag="ACHKW0_003288"
     CDS             complement(7793..9016)
                     /gene="gspF"
                     /locus_tag="ACHKW0_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7476075.1"
                     /translation="MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEA
                     RMNTSSGGMLQRRRHAHRRVAAADLALFTRQLATLVQAAMPLETCLQAVSEQSEKLHV
                     KSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
                     RQRLKSRLLQAMLYPLVLLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRTLIAM
                     SDALQASGVYWLAGLLALLVLGQRLLKNPAMRLRWDKTVLRLPVTGRVARGLNTARFS
                     RTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSSLRAALAELRLFSP
                     MMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVI
                     AILEPMLQLNNMVGM"
     gene            complement(9016..10509)
                     /gene="gspE"
                     /locus_tag="ACHKW0_003289"
     CDS             complement(9016..10509)
                     /gene="gspE"
                     /locus_tag="ACHKW0_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7476076.1"
                     /translation="MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLS
                     VLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSSEARQLMEDLGSAEDFFTLAEEL
                     PETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
                     PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVL
                     RLLDKNQARLTLERLGLSQQLTAQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELN
                     NHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQDPDVVMVGEIRDTET
                     AEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCP
                     DCRQPAPATDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRD
                     LIHRQAGELELERYVRQHSAGIRSNGIEKVLAGETSLDEVLRVTMEA"
     gene            complement(10509..12569)
                     /gene="gspD"
                     /locus_tag="ACHKW0_003290"
     CDS             complement(10509..12569)
                     /gene="gspD"
                     /locus_tag="ACHKW0_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system secretin GspD"
                     /protein_id="HFO7476077.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGPGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPATRDKMRRLIRRLDS
                     EMERSGNSQVFYLKYSKAEDLVDVLKQVSGTLTAAKEEAEGTVGSGREVVSIAASKHS
                     NALIVTAPQDIMQSLQSVIEQLDIRRAQVHVEALIVEVAEGSNINFGVQWASKDAGLM
                     QFANGTQIPIGTLGAAISQAKPQKGSTVISENGATTINPDTNGDLSTLAQLLSGFSGT
                     AVGVVKGDWMALVQAVKNDSSSNVLSTPSITTLDNQEAFFMVGQDVPVLTGSTVGSNN
                     SNPFNTVERKKVGIMLKVTPQINEGNAVQMVIEQEVSKVEGQTSLDVVFGERKLKTTV
                     LANDGELIVLGGLMDDQAGESVAKVPLLGDIPLIGNLFKSTADKKEKRNLMVFIRPTI
                     LRDGMAADGVSQRKYNYMRAEQIYRDEQGLSLMPHTAQPVLPAQNQALPPEVRAFLNA
                     GRTR"
     gene            complement(12599..13558)
                     /gene="gspC"
                     /locus_tag="ACHKW0_003291"
     CDS             complement(12599..13558)
                     /gene="gspC"
                     /locus_tag="ACHKW0_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7476078.1"
                     /translation="MARVVFRDARIYLIQWLTKIRHTLNQRQSFNTDKEHLRKIVRGM
                     FWLMLLIISAKVAHSLWRYFSFSAEYTAVSPSANKPPRADAKTFDKNDVQLISQQNWF
                     GKYQPVATPVKQPEPVPVAETRLNVVLRGIAFGARPGAVIEEGGKQQVYLQGETLGSH
                     NAVIEEINRDHVMLRYQGKIERLSLAEEGHSTVAVTNKKAVSDEAKQAVAEPAVSAPV
                     EIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDIAIA
                     LNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(13576..13986)
                     /gene="gspS2"
                     /locus_tag="ACHKW0_003292"
     CDS             complement(13576..13986)
                     /gene="gspS2"
                     /locus_tag="ACHKW0_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system pilot lipoprotein
                     GspS-beta"
                     /protein_id="HFO7476079.1"
                     /translation="MSIKQMPGRVLISLLLSVTGLLSGCASHNENASLLAKKQAQNIS
                     QNLPIKSAGYTLVLAQSSGTTVKMTIISEAGTQTTQTPDAFLTSYQRQMCADPTVKLM
                     ITEGINYSITINDTRTGNQYQRKLDRTTCGIVKA"
     gene            complement(14052..14861)
                     /gene="pppA"
                     /locus_tag="ACHKW0_003293"
     CDS             complement(14052..14861)
                     /gene="pppA"
                     /locus_tag="ACHKW0_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417446.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase PppA"
                     /protein_id="HFO7476080.1"
                     /translation="MLFDVFQQYPAAMPILATVGGLIIGSFLNVVIWRYPIMLRQQMA
                     EFHGEMSSAQSKISLALPRSHCPHCQQTIRVRDNIPLLSWLMLKGRCRDCQAKISKRY
                     PLVELLTALAFLLASLVWPESGWALAVMILSAWLIAASIIDLDHQWLPDVFTQGVLWT
                     GLIAAWAQQSPLTLQDAVTGVLVGFITFYSLRWIAGIVLRKEALGMGDVLLFAALGGW
                     VGPLSLPNVALIASCCGLIYAIITKRGSTTLPFGPCLSLGGIATLYLQALF"
     gene            complement(15009..>19483)
                     /gene="sslE"
                     /locus_tag="ACHKW0_003294"
     CDS             complement(15009..>19483)
                     /gene="sslE"
                     /locus_tag="ACHKW0_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipoprotein metalloprotease SslE"
                     /protein_id="HFO7476081.1"
                     /translation="SSSDTPPVDSGTGSLPEVKPDPTPNPEPTPEPTPDPEPTPEPTP
                     DPEPTPEPEPEPVPTKTGYLTLGGSLRVTGDITCNDESSDGFTFTPGDKVTCVAGNNT
                     TIATFDTQSEAARSLRAVEKVSFSLEDAQELAGSDNKKSNALSLVTSMNSCPANTEQV
                     CLEFSSVIESKRFDSLYKQIDLAPEEFKKLVNEEVENNAATDKAPSTHTSPVVPVTTP
                     GTKPDLNASFVSANAEQFYQYQPSEIILSEGRLVDSQGYGVAGVNYYTNSGRGVTGEN
                     GEFSFSWGETISFGIDTFELGSVRGNKSTIALTELGDEVRGANIDQLIHRYSTTGQNN
                     TRVVPDDVRKVFAEYPNVINEIINLSLSNGATLDEGEQVVNLPNEFIEQFKTGQAKEI
                     DTAICAKTDGCNEARWFSLTTRNVNDGQIQGVINKLWGVDTNYKSVSKFHVFHDSTNF
                     YGSTGNARGQAVVNISNAAFPILMARNDKNYWLAFGEKRAWDKNELAYITEAPSIVRP
                     ENVTRETATFNLPFISLGQVGDGKLMVIGNPHYNSILRCPNGYSWNGGVNKDGQCTLN
                     SDPDDMKNFMENVLRYLSNDRWLPDAKSSMTVGTNLDTVYFKKHGQVLGNSAPFAFHK
                     DFTGITVKPMTSYGNLNPDEVPLLILNGFEYVTQWGSDPYSIPLRADTSKPKLTQQDV
                     TDLIAYMNKGGSVLIMENVMSNLKEESASGFVRLLDAAGLSMALNKSVVNNDPQGYPD
                     RVRQRRSTPIWVYERYPAVDGKPPYTIDDTTKEVIWKYQQENKPDDKPKLEVASWQEE
                     VEGKQVTQFAFIDEADHKTPESLAAAKQRILDAFPGLEVCKDSDYHYEVNCLEYRPGT
                     GVPVTGGMYVPQYTQLDLGADTAKAMLQAADLGTNIQRLYQHELYFRTNGLQGERLNS
                     VDLERLYQNMSVWLWNETKYRYEEGKEDELGFKTFTEFLNCYTNNAYVGTQCSAELKK
                     SLIDNKMIYGEESSKAGMMNPSYPLNYMEKPLTRLMLGRSWWDLNIKVDVEKYPGAVS
                     EEGQNVTETISLYSNPTKWFAGNMQSTGLWAPAQKEVTIKSNANVPVTVTVALADDLT
                     GREKHEVALNRPPRVTKTYSLDASGTVKFKVPYGGLIYIKGDSKDNESASFTFTGVVK
                     APFYKDGAWKNDLNSPAPLGELESDAFVYTAPKKNLNASNYTGGLKQFANDLDTFASS
                     MNDFYGRNDEDGKHRMFTYKNLTGHKHRFANDVQISIGDAHSGYPVMNSSFSTNSTTL
                     PTTPLNDWLIWHEVGHNAAETPLTVPGATEVANNVLALYMQDRYLGKMNRVADDITVA
                     PEYLEESNGQAWARGGAGDRLLMYAQLKEWAEKNFDIKKWYPEGSLPAFYSEREGMKG
                     WNLFQLMHRKARGDEVGKTKFGERNYCAESNGNAADTLMLCASWVAQTDLSEFFKKWN
                     PGANAYQLPGASEMSFEGGVGQSAYNTLASLKLPKPEQGPETINQVTEHKMSVE"
BASE COUNT         4394 a         5501 c         4917 g         4671 t
ORIGIN      
        1 cgctggcacg gcagcgctga agcacatacc tgtcgtgaag tgcaggatat tcccggcgtg
       61 ctggctgtgt ttgccgaacg ccgtaaggac agttttggcc tgtatgtccg gctgatgagc
      121 gtcaccctga actgatgttc cgtcacccgg aaaacgagta cgactggctg agctcagacg
      181 ccgctgtgga tgaggtcctg ctcatcaacg aatacacctt taccgaagcc ggtctgcgcg
      241 ccggctgacc cgtcatcgtt aaactatcat tcaccgattt tctgtgagga ttttatcgtg
      301 tcagacacac tctccgggac aacgcatccc gatgacaatc acgaccatcc ctggtgggga
      361 ctaccctgca cagtgacgcc ctgtttcggc gtctgtctgg tgcaggaggg taaccggttg
      421 cattaccttg ccgaccgcgc cggtatcaga ggcctgttca gcgatgcaga tgcgtaccac
      481 ctggaccagg cctttccgct gctgatgaaa caactggagc tcatgctcac cagtggtgaa
      541 ctcagccccc gctatcagca taccgtcacg ctgtatgcga aaggactgac ctgcgaagcc
      601 gataccctgg gctcctgtgg ttacgtttat ctggctgttt atccgacgcc cgaaatgaaa
      661 aattaactct ccagaatagc cttctgctac ggcctggtgt tttcaccacg ccactttccc
      721 atttctcatc tctgcatatc aggaaaacct tcagtatgaa aacattaccc gatacacacg
      781 tacgggaggc atcgcgctgc ccgtctcccg tcatcatctg gcagacactg ctcacccgac
      841 tgctggacca gcactacggc ctcacgctga atgacacacc gttcgctgat gaacgtgtga
      901 ttgagcagca tattgaggca ggcatttcac tgtgtgacgc ggtgaacttt ctcgttgaaa
      961 aatacgcgct ggtacgtacc gaccagtcgg gattcagcgc ctgtacccac tctcagttaa
     1021 taaacagcat cgatatcctc cgggctcgcc gggcaacagg cctgatgact cgcgacaatt
     1081 aaagaacggt aaataacatt accctgggta aacatccagg ggcgaaacaa tgaaactggc
     1141 cctgacaccg gaagccgaca gcgttaacgt acaggcactg aacatggggc gcattgtcgt
     1201 tgacgtcgat ggtattgagc ttgctgaact gattaacgtg gtctgcgata acggtcactc
     1261 ccttcgtgtt gttgatgaat ctgaccgagc ctcaacagac tgcacgccat catttgctgc
     1321 cctgactggc atacgctgca gtaccgcgca tatcacggca aaagacaacg cctggctgta
     1381 ctcgctgtcg catcagacga atgacaccgg cgaatcagaa tggattcatt ttacaggcag
     1441 cggatatctg ttacgtaccg atgcgtggtc atacccggtt ctgcggctta agcgccaggg
     1501 cctgtcaaaa acgttccgcc gtctggttgt cacactcatc cgacgttatg gcgtcagtct
     1561 cattcatctg gatgccagcg ctgaatgcct gccgggttta cccactttcg actggtaacc
     1621 aggaacaaca tgaaatcatt aaccacggaa accgcactgg atattctgat tgcgtggctg
     1681 caggacaata tcgactgcga atcgggaatt atctttgaca acgatgagga taaaacagat
     1741 tcggcagcac tgctgccctg cattgaacag gcgagggagg atatccatac cctgcgccaa
     1801 ctgcagcttc tgcaacagaa ccggtgagtc ttactcacca gacttcattc cactgacgcc
     1861 agcctgaata cggctggcgt tttcgtttat aaaccaaaag gaaaaacgat gatgtcagat
     1921 atcacaatct cccgcccgga actggtcaac agacatacgg acgttatctg ctcaaccagt
     1981 atccgccaca ttttggctgt acgaaagagt gcgctgctgc aaatcgacac acttatccgg
     2041 caactggctg aaatctcagc aatgacagaa agtattggcg gtaaaaccgc cccggcctgg
     2101 gccatgaaac aggattttcg ctgcggctgc tggctgatgg agaaaccgga aaccgcaatg
     2161 aaagccatca cccggaatct tgaccgcgaa atctggcgtg acctgatgca acgttcaggg
     2221 atgctttcct taatggatgc acaggcccgt gatacatggt accagtcact ggagtacgat
     2281 aattttccgg aaatcagtga agcgaacatt ctgagcacat ttgaacaact gcaccagaac
     2341 aaagatgaag tgtttgagcg ggggatgatc aacgtcttca ggggactgag ctggaattac
     2401 aaaaccaatt gcccctgcaa gtttggcagt aaaattatcg tcaacaacct ggtaaggtgg
     2461 gacagatggg ggtttcatct tatcaccggg caacaggccg atcgacttgc cgatctggaa
     2521 agaatgctgc acctgttcag cggcaaaccg atccccgaca accgggaaaa catcaccatt
     2581 catctggatg accacatcca gtctgttcag tgtaaagagg actatgaaga tgagatgttc
     2641 agcatcagat actttaagaa gggctccgca cacatcacgt tcaggaaacc agaactggtt
     2701 gacaggctta atgatattat tgccaagcac tatcccggag cgttagcgat gtaaaaagga
     2761 ataaaactca ggaataaaac ccacaaaaat acacgtgttc tgatgacaaa aatcttgatc
     2821 tacttgttct cataaaaacc accagaaggg accgagggta caagagaggg tacatttttc
     2881 atcaaaccaa ataaacgaga ttaatatcaa catgataaca accatattcg attccgagtc
     2941 cggcaccact aattcttaag aacccgccca caaggcgggt ttttgctttt ggatctgaca
     3001 ataaccttca cgaaaaaaat tagcttataa agtctggggg aattactctc gccacgttaa
     3061 cgagagtaat tttattgata ttaatctcct gatactttac ccccgtccaa actccagccg
     3121 ctgcacattc accatcccag gcttctcagc agcactggca tcaatttgtg tcacccgcag
     3181 cgcatacttt tcatccagcg cgtttaacca tttcagcagg tcattaaaca ccaccggatc
     3241 tatccagacc tgaatattct ccccacgatc ggctatccgc ttgatgacta ccgagtgcgc
     3301 ggaagcactg tcactgatga cccgcgatac ctgcgcaggc gttgttgtgg cagattttcg
     3361 cgctgcaata atatccggcg cggcgttctt cagtcgggtg ttcatcgcca ccagttgctg
     3421 caacattgtc tcctgttgct caatccgttc gctcaaaggc tgccagatga ggacgtaata
     3481 tccgacgcta aacaggaaca ccaccgctgc cagtaacatg cccttttcac gcggagaacg
     3541 ccccgccagg tgttgtgcca gccagtgttc gccacggctt aactggcgtt cacgccattg
     3601 ctgaaaatag tgaataaatt tatcgcgtaa catgttattt cctccgcaac gttacgccgc
     3661 cggaaaccgc atcaccctct ttctgtaacg cgtcctgttg cacaacataa tcggttgcca
     3721 gtgcactacg cagtttgtcg aaactggcaa agttcgcggc ccgtagctgg aggtgaagta
     3781 tctggcgttt ttgatcaaag gtaaaaccac gcatttcgat gtcggaaagt gacgctgatt
     3841 tcagggtgct ggctatcgct gacaactcgg cgagcagccg ggtatcgtcg gcctgtgggc
     3901 gatatttttt cagcgccatc gtcacctgag agcgtaaatt cacaatccgc ttctgctccg
     3961 ggaacaacgt taagaactgt tcctccgcct gggtgcggct ttgcgccacc tgttcgctga
     4021 cgctccataa cgtcacgccc cgctccactg ccagcgcaac cagaatcagc aatatctgca
     4081 gaatcatcac ccgccagcgc gcccactgtt ttcggtagct gacgcgaggc tgccacggcc
     4141 ctgtcagcag gttcccttcc ggtttgccat aagtggtaac ggcgggcaga gctgtaacgg
     4201 tcaggcgttc agcgtctgcg ccagcccatg ctgatagctt ttccggtgca atgccgacta
     4261 cggttagcga aagcggtaaa tcctgctcat tgagctgggc gcggaacatg accggagcca
     4321 gcgccagccc ggcgctccat ccccggcatt catcgatgcg gcagataacc cgttgcgcat
     4381 cgcaagccat aaacccacaa ggaatggaca tccagtccgg cgcgacggta gcgcgggtga
     4441 tgccgttttc ctgcaaccac tgcgcaatgt tgcgcatatg ctgttggtga atcactgcta
     4501 cggttgccag ttgctggtcg atattcaacg gggcgaaatg cagttcatcg atatcctggt
     4561 tcagctcttc ttccagtaag gcgggcagaa tggtcggtat ctgcttacgg gcgacatcag
     4621 gcagttcaac ctgccagacg ctgatccatt cgccgggaat gtagagtcga atcgcatcgg
     4681 tttgcagcca ttgctggagc tgttcgttag caacgtcaga ccagataccg tgctccacat
     4741 cgggtgtacg gcgctgccaa tggatgggat cagcggcgca aagcgggaaa aaaatctcaa
     4801 ggatggaact cactcacttt ctcctgtctg atgccagaga accgaaaagt gttgtgggcc
     4861 catgcggaca attaacgagt tcatcgtcag ttcagtctca ttcacggtga tatctgaacg
     4921 cagccagaag taattgctgt ccacgctcag gacggttttt agctgttttt tagtacgctc
     4981 atcgacgtca gcaagtagcg gctgtgcaag aaactgatcg acatcttccc agcccttcgc
     5041 cggacgttgt tgtaataacg cccgcgcctg aacggggctt aaccacgggt caaacagcgc
     5101 ctcaagaatc acactttgcg tgacgtctaa agtattgatg ttgatttgct ggcggatcat
     5161 cggcagcgca cagaccagcg gtttcagttt ttgataaagc ccggcgtcca ttccctgtac
     5221 cacgcgcatc tcactgatat cagccagcgg ttgattcgcg gcgtagaaag gcaccgaacg
     5281 ggcgagatac tcgctgtctt cacggcccag acgtgtctgc acgctgcggt cctcgtcaat
     5341 aaactcccac aggctttcgg ctatcagttc ggcccgataa gcaggcacat ccaggcgcga
     5401 gatcagggca atcaactgtt gtaccgcaag cggacgcgac gccgttgtcg gctgagcaag
     5461 ggcattcagg ttaaagcaag cctgtgcgtc acgcagggtg accgcaattt gcccctgcgg
     5521 cagagggaag aaacgagggc cggatgccca cggctgtgcc agatgcacgc gcttttcatt
     5581 tttcaggctc agactgagcg cgctcagcgc caggctttcc gcactggcgc tgtaccacag
     5641 cgcctgctgg tactcctgct ggctgcgcgt tcgcccaagt tgttgctgca tccgcccgga
     5701 aagcgtgatg gttaccagca tcataaccgc cagcaatacc agcaccacga ccagtgccat
     5761 tccgcgtttt ggtggtgagg tgatcatgat tattgcggcc cgcgtaacaa ccagatgcgt
     5821 tcaatctcgc cccattgcgg cgaatgcagg gtcatgcgta ccgccacagg gatcgcctgt
     5881 actgacgacc agctctcctg ccagcgcgtg ccgtcgtaga actgcaaacg gagcgaatcg
     5941 gccggaatca acttttgcat tgtcggcttc acgctgcctg cggcatcggt cagcggccag
     6001 gctaaccgtt cgagataacc gtcatgaatg cggtaaccga cggtgagcag attactgcgc
     6061 ggcagacgca tcaacgggtt aaccacaccg ccgcgcacaa aacgcattcc ttcactctca
     6121 gacgccagca cgccagcgcc agccagtaac gctggttcac gctggccctg atcgcctctt
     6181 accggacgcg gcatcatttg ggtcagatcg tgggtcagaa aactcatcgt ttgctgcatg
     6241 aggtttagtt tttgatcgtg tccggcgacg gcgctattca cgcgcgtgac gccgttagtc
     6301 acctgctgcg ccatcagtgc cagcgaggca aaaatggcaa ttgccaccag catttccagt
     6361 aacgtgaaac cagcgcgagt ccttctcact gttggcctcc cacggcgcta aaccaggcgc
     6421 gtcgtgactg aatcaccgac gaaaagtctt cgtgacggct gacttcaata tccaccgcct
     6481 ggagcagcgc gttgcctgta ttcaatggga ttgttcgcca gtaccagcgg cttcctgcca
     6541 tatcgctctc accctgatgc ctgaccgccc gctgctcacg ggtcatcagg tagagcagcg
     6601 cagtctggtt ttcagctacc cagcccgcta ccgttttttc ctcaagaacg tgctgattac
     6661 tcagcgcacc gctggcaatc tgtaacaccg ccgtggcagc cagcgcaaaa atcgccagcg
     6721 cgagcatcac ttccagcaag gtaaatccgc gcttcatggc atcctctcat cacattgatt
     6781 gagggataac gcgccatcgt gtgccagctt taccgcccaa catgcgtttt gcgccgccga
     6841 gtagaagcgc agcgtaaacg gcgtggcggg ttcaaaaggc gaaaaacgga tctgcggcgt
     6901 cttctttttc gcctcttttt gcagctcaag ttcaatatcg tgcagcgtca gtcgacgacg
     6961 ttgcagctcc agcgcatatt ccttctgcca gatatcactt ccgggttgta acagcatctg
     7021 cacctgtttc ggcaccgtaa cctgtgccga taaccgggtc gaggaaacgg gcgaccactg
     7081 cccctgacga cgctgcataa actggtagcc aggcgggtcg attagcacac cgagtgtttt
     7141 cccttcgatc actgccctgt ccttaaactg cgcaaagcga gtcagaaaat cctgcgccgc
     7201 ttttttcgcg ggcgattcag aatcggtcgc aaacgtctgt actacgcccg cactggcaag
     7261 gccgataagg aaaatcacca gcatgatttc cagaagtgtg aatccgcgtt caggcactgc
     7321 ttactgaaac tcctgcaaat tccagttacc gatatctgcg ccagcgcctt ccccgttttc
     7381 ctgaccatcc gcccccaggg tataaacatc aaacagccct ttttcacccg gactgagata
     7441 ctgataatca ttgccccacg gatcctttgg cagtcgttta atgtatccac cagtacggta
     7501 gttgcgggag tccgccatat tggctggctg ctggatcagc gcctcaagcc cctgttctgt
     7561 ggtcggataa cgcccgttat ccagtcggta catatccagt gcgttttcca gcgccacgat
     7621 atcgctgatg gctttttgcc gatcggcttt ctctttgttg cccaacaggt taggcaccac
     7681 cagacttgcc aggacgccaa gaataacaat caccaccatc acttccagca gggtaaaacc
     7741 tgcccgtggt ttttgtgtgc gggataacga attcatgtga taactccgta aattacattc
     7801 caaccatatt gttcagttgc agcatcggct cgaggatggc gatgacaata aacagcacca
     7861 cgcccgccat catcaccacc agcgcaggct caaacagccc caacgccagc cccacctggg
     7921 tatcaaattc ccgttcctgg ttgactgcgg cctgctcaag catggtttcc agttcgccgc
     7981 tctgttcgcc ggaggcgatc atgtacagca tcattggcga gaacagccgc aactccgcca
     8041 gcgcggcgcg cagactgctt ccttcgcgga cgcgatctgc cgccagcagt agttgttgtt
     8101 cgacatagcg atttgccgac acggcagcag cggtctgaat gccttccagt agcggaacac
     8161 tgctggcggt gaggatgctt aaggtgcggg aaaaacgcgc cgtattcagc ccgcgcgcaa
     8221 cacgccccgt cacaggcagg cgcagcacgg ttttgtccca gcgcaggcgc atagcaggat
     8281 ttttaagtag ccgttgcccc agcaccagaa gcgccagcaa tcccgccagc cagtaaacgc
     8341 cgctggcctg taacgcatca ctcatggcaa taagcgtgcg ggtggaggag ggtagcgcgt
     8401 gtccgagatg atcaaactgt tcgatgattt tcggcaccac tgccgtcagc aaaatagtga
     8461 ctacgcccgt tgccaccacc agcagaacca gcggatagag catggcctgc aatagacgtg
     8521 atttcaggcg ctgtcgctgt tcggtgtaat ctgccaggcg attgagcacc acgtcgagat
     8581 gcccggattt ttctccggca gcaaccatcg aacaaaacag agaatcaaag acgcggggat
     8641 gttcgcgcag gctgtccgac agggtgtaac cttcctgaat ccggctgcgc agcgccattc
     8701 cgaggctttt tacatgcagt ttttcacttt gctcactgac cgcctgtaag caggtttcca
     8761 gcggcattgc tgcctgtacc agcgttgcca gttggcgcgt gaacagcgca agatctgccg
     8821 ccgccacgcg acgatgtgcg tgccgccgac gctgcaacat cccccctgac gaagtattca
     8881 tccgggcttc aatatgcacg gggataagct ctttaccgcg caacaactgg cgggcatgac
     8941 gcgcggaatc cgcctcaatc atacctttgg ttttgcgacc attacgctcc agcgcctgat
     9001 agtaaaacag tgccattacg cctccatggt tacccgcaga acttcatcga gagaggtttc
     9061 tccggcgagc actttctcaa tgccgttgct gcggataccc gcagagtgtt gtcggacata
     9121 acgttccagc tccagctccc cggcctgacg gtggatcaaa tcacgcaatg tggcatccac
     9181 cacgatcagc tcatggatgg cagtccgtcc gcgaaaacct ttgtgattac aggcgggaca
     9241 gccctgtgga tggtacagag tgacggtacg ggcgtcggta attcccagca ggcgtttttc
     9301 ttcgtcagtg gctggtgcgg gctggcggca gtcgggacac agcgtgcgaa ccagtcgctg
     9361 cgccatcacg cccgtcagac tggaagagag caggaaaggc tccacgccca tatcctgcaa
     9421 acgtgtgatc gcccccaccg ctgtgttggt atgcagcgtg gaaaggacca ggtgtccggt
     9481 aagtgaagcc tggacggcga tttctgcggt ttcggtatcg cggatttcac cgaccatcac
     9541 cacatccggg tcctgacgca aaatcgcgcg cagcccacgg gcaaaggtca tgccgacgcg
     9601 ggtgttaacc tgcgtctgac cgatcccttc aatcatgtat tcgatgggat cttcaaccgt
     9661 gagaatgttg cgcgaatggt tgttcagctc ctgcaatccg gcgtacagcg tggtgctttt
     9721 gccggaaccc gtcggccccg tcaccagaaa gatgccgtgc ggtttgtgta acagctggcg
     9781 caactgcgcg gtcagttgct ggctaagccc cagacgctcc agcgtcaggc gggcctggtt
     9841 tttgtccagc agtcgcagca ccacgcgctc gccccaggca gaaggcatgg tggagacacg
     9901 cacgtcaatc gcccggccgc ccagcaacag cgcaatacgt ccatcctgcg gcacgcgctt
     9961 ttcggcaatg tccagccgcg ccatcacctt gatacgcgac accagcagcg aggccagttt
    10021 gcgccccgga cgcagcattt catgtaatgt gccgtcaaca cgaaaacgga tcaccagact
    10081 cttttcaaac gtctcaatgt ggatatccga agcgccttct ttgattgcct ctgccagcat
    10141 ggcgttgatc agtttgatga tcggcgcatc gtcgtcgctt tccagcagat cttccgtttc
    10201 cggcagttct tccgcgaggg taaaaaagtc ctcggcagaa ccgagatctt ccatcagctg
    10261 gcgagcctcg gaagagtccc gctgccagac cgcattcagc cgctgttcaa attcggcctc
    10321 gtcgatttgc cgcagcgtaa agggcgcgtt cagcccccgc tgcagctcct gcaaaacaga
    10381 gagcgatagc ggatgaacgt ggaggatctc cagcgacgct tcgcaccatg ccaccaggct
    10441 aaaccgacgg ctgaaactgt agggcagacg cacggtgttg gcggtggttt cctgtgctac
    10501 aggcaccatt aacgcgttct cccggcattg aggaacgcgc gaacttccgg cggtaaggcc
    10561 tggttttgcg ctggcagtac cggctgtgcg gtgtgcggca tcaggcttaa accttgctca
    10621 tcacgataga tctgttcggc gcgcatatag ttatatttgc gctgcgacac gccgtctgcc
    10681 gccataccgt cacgcagaat ggtcggacgg ataaacacca tcaggttacg tttttctttt
    10741 ttatccgccg tcgatttaaa caggttacca atcaatggga tatcgcccag cagcggcact
    10801 ttcgccacgc tttctcccgc ctgatcgtcc atcagaccac caagcacaat cagctcacca
    10861 tcgttagcca gcacggtggt tttcagtttg cgctcaccaa acaccacgtc gaggctggtc
    10921 tgtccttcca ccttcgagac ttcctgctca atcaccatct gtaccgcgtt accttcgtta
    10981 atctgcggcg tgactttcag catgatgccg acttttttcc tttctactgt attgaaagga
    11041 ttgctgttat tggagccaac ggtagagccg gttaataccg gaacgtcctg gcccaccatg
    11101 aagaaggctt cctggttgtc cagcgtggtg atgctcggcg tggagagcac gttcgagctg
    11161 gagtcgtttt taaccgcctg caccagcgcc atccagtcgc ctttcaccac accaaccgcc
    11221 gtaccgctaa agccggaaag aagctgggcg agtgtggaga gatcgccgtt ggtatccgga
    11281 ttaatggtgg tagcgccgtt ttcactgatc accgtcgagc ctttctgcgg tttcgcctga
    11341 gaaatggctg cccccagcgt accgataggg atctgcgtac cattagcaaa ctgcattaat
    11401 ccggcatctt tcgacgccca ctgcacgccg aagttgatat tgctaccttc ggcaacttcc
    11461 acgatcaacg cctcgacgtg cacctgagca cggcgaatat ccagttgttc aattacgctt
    11521 tgcagcgact gcatgatgtc ctgcggcgcg gttacaatca gggcattact gtgtttgctg
    11581 gcggcgatgg atacaacctc acgcccgcta ccgaccgtgc cttccgcctc ttctttagcc
    11641 gccgtgagcg tgccgctgac ctgtttcagc acatcgacca gatcttcggc tttgctgtat
    11701 ttgagataga aaacctggct gttgccgctg cgttccattt ctgagtccag ccgacggatc
    11761 aggcggcgca ttttgtcccg cgtggccggg tcaccactga caatcacact gttggtgcgt
    11821 tcgtcggcga caatttgaga tttcagcgtc gcaggctggt tctcgccgct gtttttcgtc
    11881 aggctttcca gcacgcgggc gatttccgaa gccgaggcgt tatccagcgg gatcacctct
    11941 tcggtgcgat tacccgcgtg atccacgcgc tggatcactt ccgtcaggcg ctccacaacg
    12001 gaggcgcgtc cggtgagcat aatcacgttg gagggatcgt aattaacaac gttgcctgag
    12061 cctgcgctgt cgatcatctg gcgcagaatc ggtgccagct cgcgtaccga cacattacgt
    12121 accggcacta ctttggtgac catttcatcg cccgcgtagt tgtcgctgcc ttcaccgacc
    12181 agcggcagcg gctcgacttt cgcagcgctg gatttcacca ccttcagcac gtcgttttcc
    12241 atcggtacga cggcataccc ctgtgcttcc agcaggttaa ggaatagctg gtaatactgg
    12301 cgttcattga gcggggtcat ggtgcgaata ctcactttcc cctgtacgcc cggccccata
    12361 atgatggttt tattaaggtt agcgccgacg gtttcgatga acgatttcag gtcggtatct
    12421 ttaaaattag cggtgaaagt ggcttcttcc gcccagaccg gtgaactgca taatgccgct
    12481 gccagcacca gcggcagtaa acgctttttt gttttgaggc cagttgtctt cttacgccag
    12541 accgacaacg taatatcacg ccaaaacacg atgaatgatt ctcctggatt aaatgcggtt
    12601 agcgcagcgc gatggaaatg tcgtggcgcg cacccttgcg taaaaccgta agttgaatgg
    12661 aatccattga aggtaactgc cgcatcagag caatcattgc tcgtggatca gtgaaatcct
    12721 gctgatttag cgcaatggcg atatcgcctt ccttgaaacc gctggcatcg aacagagaac
    12781 gatctgcccc cggcttcact gcataaccga caattccctc cttacgcaca ggcgtaagct
    12841 ggatatagtt aaaaattttc tgcggatctt tcgccagtgc ctgacgcacg gcagtcggga
    12901 tctccactgg cgcactgaca gcaggttcag caacagcttg ctttgcttcg tcactgacgg
    12961 cttttttgtt ggtcacggct acggtggaat gcccctcttc tgccaggctc aggcgctcta
    13021 tttttccctg atagcgcagc atcacatggt cgcgattgat ttcctcaatc accgcattgt
    13081 gcgagccaag cgtttcaccc tgcaaataga cctgctgttt accgccttct tcaataaccg
    13141 cgccgggtct ggcaccaaag gcaatcccgc gcagcaccac attaagacgc gtttctgcca
    13201 caggcacagg ttcgggttgt tttaccggcg tggcgacggg ctgatatttg ccaaaccagt
    13261 tttgctggct gattaattgc acgtcatttt tatcgaacgt ttttgcatcc gcacggggcg
    13321 gtttattcgc cgatggggaa acagccgtat attccgcaga aaaggagaaa tagcgccaga
    13381 gtgaatgcgc cacttttgca gaaataataa gcagcatcag ccagaacatc ccgcgtacaa
    13441 tttttcgcag atgctctttg tctgtattaa aagattgtct ctggttaaga gtgtgacgaa
    13501 tttttgtcag ccattgaatg agataaattc gtgcgtcacg aaaaacaacc cgcgccaatt
    13561 tatatctacc cgacgttatg ctttgactat tccacaggtg gtacgatcca gtttccgctg
    13621 atactggtta cctgtacgtg tatcattaat cgttatgctg taattaattc cctcggtaat
    13681 cattaatttc accgttgggt cagcgcacat ttgtcgttga tagctggtta aaaaggcgtc
    13741 aggcgtctgc gtggtctgag tccccgcttc gctgataatg gtcattttta ccgtcgtgcc
    13801 gctactttgt gccagcacta aggtatatcc cgcagattta atcggcaggt tttggctgat
    13861 attttgcgcc tgttttttcg ccagtaaact ggcattttca ttatggctgg cacagccact
    13921 taataatcct gtaacgctca acaatagcga tattaatacc ctccctggca tttgttttat
    13981 cgacatagta aataatctct aaggttatta ataagagtta aaatgtcact ttgataatga
    14041 cgtggttatc attaaaacaa tgcctgtaga taaagtgttg ctataccgcc cagacttaaa
    14101 cacggtccaa aaggcagtgt ggttgatcct ctttttgtaa taatggcata tatcaggccg
    14161 cagcatgatg cgattaaagc aacattgggc agtgataacg gccccaccca gccacctaac
    14221 gcagcgaaca ataatacatc gcccatgcct aatgcttctt tacgcagaac tattccggct
    14281 atccagcgca gggagtaaaa agtgataaac cccaccagga cgccggtaac tgcgtcttgt
    14341 agcgttaacg gactctgttg cgcccatgcc gcaatcagtc ctgtccacaa tacgccctga
    14401 gtaaaaacat cgggcagcca ttggtgatcg aggtcgatga tgctcgcggc aatcagccag
    14461 gcggataata tcatcaccgc cagcgcccat ccactttccg gccagaccag actcgccagc
    14521 aaaaaagcga gtgccgtcaa taactccacc agcggataac gcttgctgat tttcgcctga
    14581 cagtcgcggc agcgcccttt gagcatcaac caggagagca gcggaatatt gtcacgaacg
    14641 cggatggtct gctgacaatg tgggcagtgc gaacgcggta gcgcaaggct tatttttgac
    14701 tgcgcactcg acatttcacc gtgaaactcc gccatttgtt ggcgcagcat gatggggtaa
    14761 cgccaaatca ccacattcaa aaaactgccg atgatcaatc ctccgacggt tgccagtatg
    14821 ggcatcgccg cggggtattg ctgaaaaaca tcaaaaagca tggttaaagg ttgtttgttg
    14881 taacttgctg gatgcgggcc tgcaagtgta tgccatacgg ctttcctcca ggcccgataa
    14941 gctggcgcaa cccggtacgc cttatttcat gccggatgcg gcgtgaacgc cttatccggc
    15001 atacaggatt actcgacaga catcttatgc tcggtaacct gattaatggt ttccggtccc
    15061 tgttccggtt tcggcagctt gagcgacgcc agcgtgttgt atgccgactg gcccacaccg
    15121 ccctcgaagc tcatctcgct cgcccccggc agctggtaag cattcgcacc cggattccat
    15181 ttcttaaaga actccgaaag atccgtctgg gcgacccagg aggcacacag catcagcgtg
    15241 tccgcagcgt taccgtttga ctcagcacag taatttcttt cgccaaactt ggttttacca
    15301 acctcatcgc cgcgtgcttt acgatgcatc aactggaaca gattccagcc tttcatccct
    15361 tcacgctcgc tgtagaacgc tggcagagag ccttctggat accatttctt gatatcaaag
    15421 tttttctctg cccattcctt cagttgtgcg tacatcagca gacggtcacc cgcaccgccg
    15481 cgcgcccatg cctgaccgtt gctctcctcc agatattccg gtgcgacggt aatatcgtca
    15541 gcgacacggt tcatcttgcc gagataacga tcctgcatgt acagcgccag cacgttgttc
    15601 gccacttcgg tcgcgcctgg aacagtcagc ggcgtttctg cggcgttgtg ccctacttcg
    15661 tgccagatca gccagtcgtt cagcggcgtc gtcggcagcg tggtgctgtt cgtcgagaag
    15721 ctgctgttca ttaccggata acccgaatgc gcatcaccga tggagatctg cacatcgttg
    15781 gcgaaacgat gcttgtggcc cgtcaagttt ttataggtaa acatccggtg cttaccgtct
    15841 tcatcattac gaccgtagaa atcattcatc gagctggcaa aggtatccag atcgttagcg
    15901 aattgcttca gtccgccagt gtaattgctg gcattcaggt tcttcttcgg tgcggtataa
    15961 acgaaagcgt ctgattccag ctcacccagc ggagccggtg agttcagatc gtttttccat
    16021 gcgccgtctt tatagaacgg tgcttttacc acaccagtaa aggtgaagct ggcagattca
    16081 ttgtctttgc tgtcgccttt gatataaatc agaccaccgt aaggcacctt gaatttcacc
    16141 gtaccactag cgtccagaga gtacgttttg gttactcttg gcggacggtt cagcgcgact
    16201 tcatgcttct ctcttccggt caggtcgtca gccagcgcca cggtgacggt cacaggaacg
    16261 ttcgcattgg acttaatggt gacctctttc tgagccggtg cccacaggcc agttgactgc
    16321 atgttacctg caaaccattt ggtcggattc gagtacaggc tgatggtttc agtaacgttc
    16381 tgtccctctt ccgatactgc tcccggatac ttctcgacat caactttgat gttcagatcc
    16441 caccaggaac ggcccagcat caggcgtgtc agcggttttt ccatatagtt gagcgggtag
    16501 ctcgggttca tcatgcccgc tttgctgctt tcttcaccgt aaatcatctt gttatcgatc
    16561 agcgattttt tcagctcagc ggaacactgc gtgccaacgt atgcattgtt ggtgtagcag
    16621 ttcagaaact cagtgaacgt tttaaagccc agctcgtctt ctttaccctc ttcataacga
    16681 tatttcgtct cgttccacag ccagacggac atgttttggt ataaacgttc cagatcgacg
    16741 ctgttgagac gctcaccctg gaggccattg gtacggaaat aaagctcatg ctgatacagg
    16801 cgctgaatat tggtgcctaa atccgcagcc tgcagcatcg ctttcgcagt gtcagctcca
    16861 agatccagct gcgtatactg cggaacatac atgccaccgg ttaccggcac acccgtgcct
    16921 gggcggtatt ccagacagtt gacctcatag tgatagtcag aatccttaca cacttccagc
    16981 cctgggaacg cgtccagaat tctctgtttt gccgcagcca gtgactcagg cgttttgtgg
    17041 tcggcttcat caataaaggc gaattgggtt acctgtttac cctcaacttc ttcctgccag
    17101 ctggcaactt ccagcttcgg tttgtcatca ggtttgtttt cttgctgata tttccagata
    17161 acttctttcg tggtgtcatc aatggtatac ggtggtttac catcgacagc cggataacgc
    17221 tcatagaccc aaattggcgt tgaacgtcgt tgacgaacac ggtcagggta gccttgcgga
    17281 tcgttattta ctaccgactt gttaagcgcc atcgacaaac ctgcggcatc aagcagacgt
    17341 acaaagccag atgcgctctc ttccttaaga ttgctcatca cgttttccat gatcagcacc
    17401 gatccacctt tgttcatata ggcgatcaaa tcggtcacat cctgctgggt cagcttcggt
    17461 ttgctggtat ctgcgcgcag aggaatggag taaggatcgc taccccattg tgtgacgtat
    17521 tcaaagccat tgaggatcaa cagaggaact tcatctggat tcagattgcc atagctggtc
    17581 attggtttga ccgtgatgcc agtgaaatcc ttgtggaacg caaacggtgc gctatttccc
    17641 agcacctgac catgtttttt gaaatatacc gtgtccaggt tagtacccac ggtcatactg
    17701 gattttgcat ccggcaacca gcgatcattt gacagatagc gcagcacgtt ctccatgaag
    17761 ttcttcatgt catccgggtc gctgttgagc gtacactgtc cgtctttatt aacgcccccg
    17821 ttccagctgt aaccgttcgg gcaacgcagg atgctgttgt agtgtgggtt accgataacc
    17881 atcagcttgc catcgcccac ttgccccagc gagataaacg gcaggttgaa ggtggcggtt
    17941 tcgcgtgtca cgttctctgg tcgcacaatg gaaggcgctt cagtaatgta cgccagctca
    18001 tttttatccc aggcgcgttt ttcgccaaac gccagccagt agtttttatc attacgcgcc
    18061 atcagaatcg ggaaggccgc gttggagata ttcaccaccg cctgaccgcg cgcattaccc
    18121 gtgctgccat agaagttagt agagtcatgg aatacatgga acttgctgac agatttgtag
    18181 ttcgtatcca cgccccacag cttgttgata acgccctgaa tctggccgtc attaacattg
    18241 cgcgtcgtca gcgagaacca gcgagcctcg ttacaaccgt cggttttcgc acaaatcgcg
    18301 gtatcgatct ctttggcctg acccgtctta aactgctcga taaattcgtt tggcagatta
    18361 acaacttgct caccttcatc cagcgtcgca ccgttggata acgagagatt gataatctcg
    18421 ttgatcacgt tgggatattc ggcaaagacc ttgcgtacat catccggaac aacacgggta
    18481 ttattttgcc cggtcgtcga atagcgatga ataagctgat cgatattcgc cccgcgaact
    18541 tcatcaccca gttcagtcag cgcaatggtc gacttattgc cgcgcactga acccagttca
    18601 aaggtatcga taccaaagga gatggtttcg ccccagctaa aagaaaattc accattttcc
    18661 cctgtcacgc cacggcctga attggtgtag tagttgacgc cagcaacacc atatccctgg
    18721 ctatccacca ggcgaccttc tgagagaatg atttccgaag gttgatactg ataaaactgt
    18781 tccgcgttag ccgacacgaa ggaggcgttc agatccggtt ttgttcccgg agtcgtgacg
    18841 gggactaccg gtgaagtatg agtggatggc gctttatcag tcgcggcatt gttttccacc
    18901 tcttcattga ccagcttttt gaactcttcc ggtgccagat cgatttgctt atacagcgag
    18961 tcgaaacgtt tactctcgat caccgaggag aactccaggc acacctgttc tgtattcgcc
    19021 gggcaactgt tcatggaggt gaccagcgag agcgcattgc ttttcttgtt gtcggaaccc
    19081 gccagttctt gcgcgtcctc aagactaaac gacacttttt caaccgcacg caggctacgc
    19141 gcagcttctg actgggtgtc gaaggtagca attgtcgtgt tgttccctgc cacacaggtg
    19201 actttgtcgc ctggtgtaaa ggtaaagcca tcgctggatt catcattaca ggtgatatca
    19261 ccagttaccc gcaggcttcc gcccagggtc agataacccg ttttcgtagg aacaggttct
    19321 ggctccggtt ctggtgttgg ctcaggatca ggtgtcggtt ccggcgtagg ttctgggtcc
    19381 ggcgttggct caggcgtcgg ctccgggttt ggtgttggat caggtttcac ttccggcaaa
    19441 gaccctgttc cagaatctac aggcggcgta tcggaggaag atc
//
LOCUS       DAZFXT010000086        19365 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.086, whole genome shotgun sequence.
ACCESSION   DAZFXT010000086 DAZFXT010000000
VERSION     DAZFXT010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.086"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            443..1582
                     /gene="wza"
                     /locus_tag="ACHKW0_003295"
     CDS             443..1582
                     /gene="wza"
                     /locus_tag="ACHKW0_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HFO7476082.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKIHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1588..2031
                     /gene="wzb"
                     /locus_tag="ACHKW0_003296"
     CDS             1588..2031
                     /gene="wzb"
                     /locus_tag="ACHKW0_003296"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HFO7476083.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2034..4196
                     /gene="wzc"
                     /locus_tag="ACHKW0_003297"
     CDS             2034..4196
                     /gene="wzc"
                     /locus_tag="ACHKW0_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310892.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HFO7476084.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQSSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4374..5213
                     /gene="wcaA"
                     /locus_tag="ACHKW0_003298"
     CDS             4374..5213
                     /gene="wcaA"
                     /locus_tag="ACHKW0_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HFO7476085.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5216..5704
                     /gene="wcaB"
                     /locus_tag="ACHKW0_003299"
     CDS             5216..5704
                     /gene="wcaB"
                     /locus_tag="ACHKW0_003299"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HFO7476086.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGDNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5701..6918
                     /gene="wcaC"
                     /locus_tag="ACHKW0_003300"
     CDS             5701..6918
                     /gene="wcaC"
                     /locus_tag="ACHKW0_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HFO7476087.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDITLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            6893..8110
                     /gene="wcaD"
                     /locus_tag="ACHKW0_003301"
     CDS             6893..8110
                     /gene="wcaD"
                     /locus_tag="ACHKW0_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid polymerase WcaD"
                     /protein_id="HFO7476088.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWSAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8121..8867
                     /gene="wcaE"
                     /locus_tag="ACHKW0_003302"
     CDS             8121..8867
                     /gene="wcaE"
                     /locus_tag="ACHKW0_003302"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HFO7476089.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8883..9431
                     /gene="wcaF"
                     /locus_tag="ACHKW0_003303"
     CDS             8883..9431
                     /gene="wcaF"
                     /locus_tag="ACHKW0_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HFO7476090.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKT
                     LPANVVCRGNPAVVIRERVETE"
     gene            9458..10579
                     /gene="gmd"
                     /locus_tag="ACHKW0_003304"
     CDS             9458..10579
                     /gene="gmd"
                     /locus_tag="ACHKW0_003304"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HFO7476091.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10582..11547
                     /gene="fcl"
                     /locus_tag="ACHKW0_003305"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10582..11547
                     /gene="fcl"
                     /locus_tag="ACHKW0_003305"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HFO7476092.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11550..12029
                     /gene="gmm"
                     /locus_tag="ACHKW0_003306"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11550..12029
                     /gene="gmm"
                     /locus_tag="ACHKW0_003306"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HFO7476093.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEDLILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            12026..13249
                     /gene="wcaI"
                     /locus_tag="ACHKW0_003307"
     CDS             12026..13249
                     /gene="wcaI"
                     /locus_tag="ACHKW0_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HFO7476094.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13252..14688
                     /gene="cpsB"
                     /locus_tag="ACHKW0_003308"
     CDS             13252..14688
                     /gene="cpsB"
                     /locus_tag="ACHKW0_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HFO7476095.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     TRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14881..16251
                     /gene="cpsG"
                     /locus_tag="ACHKW0_003309"
                     /gene_synonym="manB"
     CDS             14881..16251
                     /gene="cpsG"
                     /locus_tag="ACHKW0_003309"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HFO7476096.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16306..17700
                     /gene="wcaJ"
                     /locus_tag="ACHKW0_003310"
     CDS             16306..17700
                     /gene="wcaJ"
                     /locus_tag="ACHKW0_003310"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HFO7476097.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSMIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17702..19180
                     /gene="wzxC"
                     /locus_tag="ACHKW0_003311"
     CDS             17702..19180
                     /gene="wzxC"
                     /locus_tag="ACHKW0_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HFO7476098.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYRPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLGVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
BASE COUNT         4654 a         4855 c         5337 g         4519 t
ORIGIN      
        1 cctaaaaacc atattgaatg acacttaata taattcttaa aaatagccaa ttgccgaatt
       61 gttaccttgc ctgctattcc gttagctgta acacttcctc ctgcattatt ggagagccaa
      121 tattcaatta ccataatgtc accttatatt cggaagttaa tttccgcaat aaccctttct
      181 catcaacgat tgtatgcaga gagggtttga aaaattaagc taaatcagtg tattggtagc
      241 taaaaagcca ggggcggtag cgtgtctgga tgcctgaaag accagtctga attattctgt
      301 caatagtctg cggatgaaat caattttttt aggactgatg ccagttaaat ttttattcaa
      361 ttaactgcaa ggtaaatgtg catggcgaca ttgctattaa tagtgcacag gataattact
      421 ctgccaaagt gataaataaa caatgatgaa atccaaaatg aaattgatgc cattattggt
      481 gtcagtaacc ttgataagcg gttgcacagt acttccgggc agcaatatgt cgacgatggg
      541 caaagacgtc atcaaacagc aggacgctga tttcgatctc gacaaaatgg tgaatgttta
      601 tccgctgacc ccgcgcctga ttgaccaatt acgcccacgc ccgaatgtag cgcgccccaa
      661 tatgacgctg gaaagtgaga tcgcgaatta ccagtatcgc gtcgggccgg gagacgttct
      721 taatgtcacc gtctgggatc acccggaact caccacgcca gctggtcagt accgcagctc
      781 cagcgacacc ggcaactggg tacagcctga cggcactatg ttctacccgt atatcggcaa
      841 gattcacgtc gtggggaaaa cgctcgctga aatccgcagt gatattaccg ggcgcttagc
      901 gacgtacatc gctgacccgc aggtggacgt taatatcgcc gccttccgct cacaaaaggc
      961 ctatatctcc ggtcaggtga ataaatccgg tcaacaggcg atcaccaacg taccactgac
     1021 cattctcgat gccatcaacg ccgcaggtgg cctgaccgac accgctgact ggcgcaacgt
     1081 ggtgctaaca cacaatggtc gtgaagaacg catttctttg caggcgctga tgcaaaacgg
     1141 cgacctcaat cagaaccgac tgctttaccc cggcgatatt ctctacgtgc cgcgtaatga
     1201 tgatctgaaa gtatttgtga tgggtgaagt gaagaaacag agcaccctga aaatggactt
     1261 tagcggcatg accctgacgg aagccttggg taatgctgaa ggcatcgaca tgaccacctc
     1321 caacgccagc ggcatctttg tcattcgtcc gctgaaaggc gagggcgggc gtaacggcaa
     1381 gattgccaat atctaccagc tggatatgtc cgatgcgacg tcgctggtga tggcgacaga
     1441 attccgcctg caaccttatg acgtggtgta tgtcaccacc gccccggttt cccgctggaa
     1501 ccgtctgatc aatcagttgc tgccaactat tagcggtgtc cgttacatga cggatacagc
     1561 cagcgacatt cataactggt aatcgtcatg tttaacaaca ttttagttgt ctgtgtcggc
     1621 aatatttgcc gttccccgac ggcggaacgc ttactgcaac gttatcaccc ggaactgaaa
     1681 gtggagtccg ctggactcgg cgcgctggtc ggtaagggcg ctgaccctac tgctatcagc
     1741 gtcgccgcag aacatcaact gtctctggaa ggtcactgtg cccgtcaaat cagccgccgc
     1801 ctgtgtcgca actacgacct gattttgacc atggaaaagc gccatatcga acgcttatgc
     1861 gagatggcac ccgagatgcg cggcaaagtg atgctgtttg gtcactggga taacgaatgt
     1921 gaaatccccg atccgtatcg caaaagccgg gaaacgtttg cagcggtgta cacattactt
     1981 gaacggtctg cccgccagtg ggcgcaggca ttgaacgcag agcaggtata agaatgacag
     2041 aaaaagtaaa acaacatgcc gctccggtaa cgggcagtga tgaaatcgat attggtcgcc
     2101 tggtcggcac cgtcattgaa gcgcgctggt gggtgattgg catcaccgcc gtatttgccc
     2161 tctgtgccgt ggtttacact ttcttcgcca cgccgattta tagtgccgac gcactggtac
     2221 aaatcgagca aagcagcggc aattcgttag tgcaggacat tggttcggca ttagccaaca
     2281 aaccgcctgc atcggacgcc gagatccagt tgattcgttc gcgtctggtg cttggtaaaa
     2341 cggtggacga tctcgacctc gatattgcag tgagcaaaaa cacgttcccg attttcgggg
     2401 cgggctggga tcgcctgatg ggacgccaga acgagacggt gaaagtgact acctttaacc
     2461 gcccgaaaga gatggcggat caggtgttta cgcttaatgt gctggatgac aaaaactaca
     2521 ccctgagcag tgatggcggc tttagcgccc gtggacaagc gggccagatg ctgaaaaaag
     2581 aaggcgtcac gctgatggtt gaagccattc acgcccgccc gggcagtgag tttaccgtca
     2641 ccaaatactc cacgctgggg atgatcaatc aactgcaaaa cagcctgacg gtaacggaga
     2701 acggcaaaga cgcaggcgta ctgagcctga cttataccgg tgaagatcgc gaacagatcc
     2761 gcgacattct taatagcatc gcccgtaact atcaggaaca aaatattgag cgcaaatcgg
     2821 cggaagcgtc gaaaagcctc gctttcctcg cgcaacagtt accggaagta cgtagccgcc
     2881 ttgatgttgc cgaaaacaaa ctgaatgcct tccgtcagga taaagattct gttgatctgc
     2941 cgctggaagc gaaagcggtg ctcgattcga tggtgaacat cgacgcccag ttgaacgaac
     3001 tgacctttaa agaggcggaa atctccaagc tgtacaccaa agttcacccg gcgtatcgca
     3061 cgctgctgga gaaacggcag gcgctggaag acgaaaaagc caaacttaac ggtcgcgtaa
     3121 cggcgatgcc gaaaacccag caggaaattg tccgtctgac ccgcgatgtc gagtctgggc
     3181 agcaggtcta tatgcaactg ctgaataaag agcaggagct gaaaatcacc gaagccagca
     3241 ccgtcggcga tgtgcgcatt gttgacccgg caatcaccca gcctggcgtg ctgaaaccga
     3301 agaaaggact gattatcctc ggcgcaatta tcctcggcct gatgctctct atcgtgggtg
     3361 tgctgctgcg ctcgttgttt aatcgcggca tcgaaagccc gcaggtgctg gaagaacatg
     3421 gtatcagcgt ctatgccagc atcccgttgt cggaatggca gaaagcgcgc gatagcgtca
     3481 aaaccatcaa agggattaaa cgctataaac agagccagct actggcggtg gggaatccaa
     3541 ccgatctggc gattgaagcc atccgcagtc tgcgtaccag tctgcacttc gcgatgatgc
     3601 aggcgcagaa caatgtgttg atgatgacag gggtgagccc gtcaatcggt aaaacctttg
     3661 tctgcgccaa cctggcggcg gtgatcagtc agaccaataa acgggtgttg ttgatcgact
     3721 gcgatatgcg caaaggctac acccacgagc tgttgggtac caataacgtc aatggcctgt
     3781 cggaaattct gattggccag ggcgatatta ctaccgcagc taaaccgacc tctattgcca
     3841 aatttgacct gatcccgcgc ggtcaggtac cgccaaatcc ttctgaactg ttgatgagcg
     3901 aacgctttgc cgaactggtg aactgggcga gtaaaaacta tgacctggtg ttgattgata
     3961 cgccgccgat tctggcagtg accgatgcgg caattgttgg tcgtcatgtt ggaaccacgt
     4021 taatggtggc gcgttatgcg gtcaacacat tgaaagaagt ggaaaccagt ctgagccgct
     4081 ttgagcaaaa tggtattccg gtgaaagggg tgattctgaa ctccatcttc cgccgcgcca
     4141 gcgcgtatca ggattatggc tattacgaat acgaatataa gtcggatgcg aaataacgac
     4201 atccgctgtc ggatgcgatg ctgacgcatc ttatccgacc taccttcacg ccgcatccgg
     4261 caagcaaacc agctccgtag gccggataag gcgctcacgc cgcatccggc aacagaaccc
     4321 gtaaacaagc ttataaaccg gcagaacatc tcgacactcc gggagaacaa cctatgaaag
     4381 acaatccgct gatttcaatc tatatgccga cctggaaccg ccaacaactg gcgattcggg
     4441 cgataaaatc ggtcctgcgc caggactaca gcaactggga gatgatcatc gtggatgatt
     4501 gctccacctc ctgggaacaa ctgcaacagt acgtcactgc cctcaacgat ccgcgtatta
     4561 cctacattca caacgacatt aacagcgggg cgtgcgcggt acgtaaccag gcgattatgc
     4621 tggcgcaagg ggaatacatt accgggatcg atgacgatga tgaatggacg cccaaccgcc
     4681 tgagcgtctt cctcgcccat aaacagcaac tggtcactca cgcctttttg tacgctaacg
     4741 actacgtttg ccagggcgaa gtctattccc aaccagcaag cttaccactg tatcccaaat
     4801 cgccgtactc acgccgcctg ttctataaac gcaatatcat tggcaatcag gtttttacct
     4861 gggcgtggcg tttcaaagag tgcttgttcg ataccgaact gaaagcagca caggattacg
     4921 acatcttcct gcggatggtg gtggagtatg gcgaaccgtg gaaagtggaa gaggcgacgc
     4981 agatcctgca tatcaatcac ggtgaaatgc agatcacctc gtcaccgaaa aaattctccg
     5041 ggtacttcca cttttaccgc aagcacaaag acaaattcga ccgtgccagt aaaaaatatc
     5101 agctgtttac cctctaccag atccgcaata aacgcatgac ctggcgcact ttgctgacgc
     5161 tgctgtcggt gcgtaacggc aaacgtctgg ctgatggtat tcgggggcgc taaagatgct
     5221 ggaagatctg cgcgccaaca gctggagttt acgcccgtgc tgcatggttc ttgcctatcg
     5281 cgttgctcat ttttgttcgg tgtggcgcaa aaagaacgtc ctcaacaatc tgtgggcggc
     5341 cccgctgctg gtgctgtatc gcattatcac cgaatgcttt ttcggttatg aaatccaggc
     5401 agccgcaacc attggccgcc gctttactat ccatcacggt tacgccgtag tgatcaataa
     5461 aaacgtggtt gcgggggatg atttcaccat tcgccacggc gtcactatcg gcaatcgggg
     5521 tgccgataac atggcatgtc cacacattgg caacggcgtc gaactcggtg ccaacgtcat
     5581 tattcttggt gatatcacgc ttggtgacaa cgtcaccgtg ggcgcgggca gcgtggtgct
     5641 cgactctgtc ccggacaacg cgctggtggt gggagaaaaa gcgcgagtga aggtaattaa
     5701 atgaatattt tgcaatttaa tgtgcgactg gcggaaggcg gggcagcagg tgtggcgtta
     5761 gatctccacc agcgtgcgct gcaacagggg ctggcgtcac attttgtcta cggttacggc
     5821 aaaggcggca aagagagcgt cagccatcag aactatccgc aggtcatcaa acatacgccg
     5881 cggatgaccg cgatggcgaa tattgccctg tttcgtctgt ttaatcgcga tctgtttggc
     5941 aatttcaatg agttatatcg caccattact cgcacaccgg gtccggtggt cctgcatttt
     6001 catgtgctgc acagctactg gctaaatctt aagagcgtgg tgcgcttttg cgaaaaagtg
     6061 aaaaaccaca aaccggatat cactctggtc tggacgctgc acgatcactg gagcgttacc
     6121 ggacgctgcg cctttaccga cggttgcgaa ggctggaaaa cgggctgcca gaaatgcccg
     6181 accttaaata actatccgcc ggtgaagatt gatcgcgcgc atcagctggt ggcgggcaaa
     6241 cgccagttat tccgcgagat gctggcgctg ggctgtcagt ttatttcccc cagccagcat
     6301 gtggctgacg ctttcaatag tctgtacggg ccagggcgtt gccggattat caataatggc
     6361 attgatatgg caaccgaagc gattctggca gatctgcctc cggtgcgtga aacccagggc
     6421 aagccgaaaa tcgcggtggt ggcgcatgac ttgcgttacg acggcaagac taaccagcaa
     6481 ctggtgcgtg agatgatggc gctgggcgac aaaatcgaac tgcatacctt tggtaagttc
     6541 tcgccgttca ccgctggcaa cgtggttaat cacggctttg aaactgacaa gcgcaagttg
     6601 atgagcgcgc tcaatcagat ggatgcgctg gtattcagtt ctcgcgtcga taactacccg
     6661 ctgattttgt gtgaggcgtt atcaattggc gtgccggtga ttgccaccca tagcgacgcg
     6721 gcacgggaag tgttgcaaaa atccggcggt aaaaccgtcg acgaagaaga ggtgctgcaa
     6781 ctggtccagt taagcaaacc ggaaatcgcg caggcgatat ttggtaccac gctggctgag
     6841 ttcagccaac gcagccgcgc cacctacagt ggacaacaga tgctggagga gtatgtcaac
     6901 ttctatcaga atctgtagct acctgctgct gccgctgatc tacctgctgg ttaacgtcaa
     6961 aatcgcccag cttggcgaaa gtttccccat cactatcgtc acttttttac ctgtcttgtt
     7021 actgctgttt ttagaacgca tcagcgttaa aaaattgatg attgccttag ggattggtgc
     7081 gggactcacg gcgttcaact atctgtttgg tcagtcgttg gatgccagta aatacgtcac
     7141 atcaaccatg ctgtttgtct atattgtgat cattattggc atggtgtgga gtattcgctt
     7201 taaaacgatt tcgccacaca accatcgcaa gatattacgt ttcttttatc tggtggtcgg
     7261 gctggtggtg gcgctggcgg cggtggagat ggcgcaaatt atcctcaccg gtggcagcag
     7321 tattatggag tcgatttcga aatatctgat ttacagcaac agctatgtac tgaatttcat
     7381 taaattcggc ggcaagcgca cgacagcact ttatttcgaa ccggcatttt tcgctctggc
     7441 attaatctca atttggctca gcatcaaaca gtttggtatc aaaacgccta aaaccgatgc
     7501 tatgattctc gcagggataa tattatccgg atcgttttca ggggttatga cctttatcct
     7561 gttttatttg ctggagtggg catttcaata tctgaataaa gaggcgatta agaaaaagtt
     7621 accgttggca ttgatttctc tggctgtatt cctggttggt gtggtaatcg cgtttcctta
     7681 tatttccacc cgtctgggcg atttaggtac ggaaggatcg tcatcgtatt atcgtattgt
     7741 cggtccgctg gtgatggtcg gttattcttt gacccatatt gacggtgtag tcagatttgg
     7801 ctcactttat gaatacgtcg catcattcgg aatatttaac ggtgcggatg tcggaaaaac
     7861 catagacaat ggtttgtatc tgctgattat ttatttttcc tggtccgcag tgtttttatc
     7921 gctgtggtac atggggaaag tgataaaaat gatgatcaac gcttttggtg ataaccgcaa
     7981 ttttcgcgtg caattatatc tttttactcc ggtatcgctg ttttttaccg gttcgatatt
     8041 tagcccggaa tatgcatttt taatcgtctg tccgtttatt ttgcgaaaag cgttaaatat
     8101 tacgaggtaa gaataagaac atgttgctta gcataatcac tgtcgcgttt cgtaacctcg
     8161 aagggatagt caaaacacat gcctcgctgg cgcatctggc gcaggcggaa gatatcagct
     8221 tcgaatggat tgttgtcgat ggcggttcca atgacggcac ccgtgagtat ctggaaaatc
     8281 tcaatggtat ctataaccta cgctttgtca gcgagccaga taacggtatc tacgacgcca
     8341 tgaacaaagg cattacgatg gcacaaggca agttcgcgtt gtttctcaat tcgggcgata
     8401 tttttcatca ggatgccgca aattttgtcc gtaagttaaa aatgcaaaaa gataacatga
     8461 tgatcaccgg cgatgcgctg ctggattttg gcgacgggca taaaattaaa cgtagcgcca
     8521 aaccgggctg gtatatttat cacagcctgc ccgccagtca tcaggcgata tttttccctg
     8581 tatccggctt gaaaaaatgg cgttatgacc tggaatataa agtttcttcc gactacgcgc
     8641 tggcagccaa aatgtataaa gccggttatg catttaaaaa actcaatggc ctggtgtctg
     8701 aattttccat gggtggggta tctaccacca ataatatgga attgtgtgct gacgcgaaaa
     8761 aagtccaacg acaaatatta catgtacctg gcttttgggc tgaattatcc tggcatttac
     8821 gccaacgtac tacctcaaag acgaaagcct tatataacaa agtctgaata taaggagaac
     8881 caatgcaaga tttaagcggt ttctcggtgc cgaaagggtt ccggggcggc aacgctatta
     8941 aagtgcaatt atggtgggca gtacaggcaa caatatttgc ctggtcgcca caagtattgt
     9001 atcgctggcg ggcattttta ttacgtttat tcggcgcaaa aataggaaaa aacgtagtta
     9061 ttcgtccgtc agtaaaaatt acctatccgt ggaaattaac cttaggtgat tacgcgtggg
     9121 tcggcgatga cgtcaattta tataccctcg gtgaaataac cattggcgca cattcggtga
     9181 tatcgcaaaa aagttattta tgcaccggta gccacgacca tgcaagtcaa catttcacca
     9241 ttaatgctac gcctattgtg attggcgaga aatgctggct ggcaaccgat gtttttgttg
     9301 cccctggcgt cacgattggc gacggcaccg tcgtgggtgc acgaagcagt gtttttaaaa
     9361 cgcttccggc aaatgtggtt tgccggggga atcccgcagt ggtgatacgc gaacgcgttg
     9421 aaactgaata aattcaaaaa atacagagga ataagacatg tcaaaagtcg ctctcatcac
     9481 cggtgtaacc ggacaagacg gttcttacct ggcagagttt ctgctggaaa aaggttacga
     9541 ggtgcatggt attaagcgtc gtgcatcgtc attcaacacc gagcgcgtgg atcacattta
     9601 tcaggatccg cacacctgca acccgaaatt ccatctgcat tatggcgacc tgagtgatac
     9661 ctccaacctg acacgcattt tgcgtgaagt gcagccggat gaagtttaca acctgggcgc
     9721 aatgagccac gttgcggttt cttttgagtc accggaatat accgcagacg tcgatgcgat
     9781 gggtacgctg cgcctgctcg aggcgatccg cttcctcggt ctggaaaaga aaacccgttt
     9841 ctatcaggct tccacctctg aactgtatgg tctggtgcag gaaattccgc agaaagaaac
     9901 cacgccgttc tacccacgct ctccgtatgc tgtcgccaaa ctgtacgcct actggatcac
     9961 cgttaactac cgcgaatcct acggcatgta cgcctgtaac ggtattctct tcaaccatga
    10021 atccccgcgc cgcggcgaaa ccttcgttac ccgcaaaatc acccgcgcaa tcgccaatat
    10081 cgcccagggg ctggagtcgt gcctgtacct cggcaatatg gattccctgc gtgactgggg
    10141 ccatgccaaa gactacgtaa aaatgcagtg gatgatgctg caacaggaac agccggaaga
    10201 tttcgttatc gcgaccggcg ttcagtactc cgtgcgtcag ttcgtggaaa tggcggcagc
    10261 acagctgggc atcaaactgc gctttgaagg cacgggcgtt gaagagaagg gcattgtggt
    10321 ttccgtcacc gggcatgacg cgccgggcgt taaaccgggc gatgtgatta tcgccgttga
    10381 cccgcgttac ttccgtccgg cagaagttga aacgctgctc ggcgacccga ccaaagcgca
    10441 cgaaaaactg ggctggaaac cggaaatcac cctcagagag atggtgtctg aaatggtggc
    10501 taatgacctc gaagcggcga aaaaacactc tctgctgaaa tctcacggct acgacgtggc
    10561 gatcgcgctg gagtcataag catgagtaaa caacgcattt ttatcgctgg ccatcgtggg
    10621 atggtcggtt ctgccatcag gcggcagctc gaacagcgcg gtgatgtgga actggtatta
    10681 cgcacccgcg acgagctgaa cctgctggac agccgcgcgg tgcatgattt ctttgccagc
    10741 gaacgcattg accaggtcta tctggcggcg gcgaaagtgg gcggcattgt tgccaacaac
    10801 acctatccgg cggatttcat ctaccagaac atgatgattg agagcaacat cattcacgcc
    10861 gcgcatcaga acgacgtgaa caaactgctg tttcttggat cgtcctgtat ctacccgaaa
    10921 ctggcaaaac agccgatggc agaaagcgag ttattgcagg gcacgctgga gccgaccaac
    10981 gagccttacg ccattgccaa aatcgccggg atcaaactgt gcgaatcata caaccgccag
    11041 tacggacgcg attaccgctc agtcatgccg accaacctgt atggcccgca tgacaacttc
    11101 cacccgagta attcgcatgt gatccctgca ttgctgcgtc gcttccacga ggcgacggca
    11161 cagaatgcac cggacgtggt ggtatggggc agcggtacac cgatgcgtga attcctgcac
    11221 gtcgatgata tggcggcggc gagcattcat gtcatggagc tggcgcacga agtctggctg
    11281 gagaacaccc agccgatgct gtcgcacatt aacgtcggca cgggcgttga ctgcactatc
    11341 cgcgagctgg cgcaaaccat cgccaaagtg gtgggttaca aaggtcgggt ggtttttgat
    11401 gccagcaaac cggatggtac gccgcgcaaa ctgctggatg tgacgcgcct gcatcagctt
    11461 ggctggtatc acgaaatctc actggaagcg gggcttgcca gcacttacca gtggttcctt
    11521 gagaatcaag accgctttcg ggggtaatga tgtttttacg tcaggaagac tttgccacgg
    11581 tagtgcgctc caccccgctt gtctctctcg actttattgt cgagaacagt cgcggcgagt
    11641 ttctgcttgg caaaagaacc aaccgcccgg cgcagggtta ctggtttgtg ccgggagggc
    11701 gcgtgcagaa agacgaaacg ctggaagccg cattcgagcg gctgacgatg gcggaactgg
    11761 ggctgcgtct gccgataaca gcaggccagt tttacggtgt ctggcagcac ttttatgacg
    11821 ataacttctc cggcacggat ttcaccactc actatgtggt gctcggtttt cgcttcagag
    11881 tcgctgaaga agatctgata ctgccggatg agcagcatga cgattaccgc tggctgacgc
    11941 cggacgcgct gctcgccagt aacgatgttc acgctaacag ccgcgcctat tttctcgctg
    12001 agaagcgtgc cggagtaccc ggattatgaa aatactggtc tacggcatta actactcgcc
    12061 ggagttaacc ggtatcggca aatacaccgg cgagatggtg gaatggctgg cggcacaagg
    12121 tcatgaggtg cgggttatta ccgcaccgcc ttactacccg cagtggcagg tgggcgagaa
    12181 ctattccgcc tggcggtaca aacgagaaga gggggccgcc acggtgtggc gctgcccgct
    12241 gtacgtgcca aaacagccga gcaccctgaa acgcctgtta catctcggca gttttgccgt
    12301 cagcagtttc tttcctctga tggcgcaacg tcgctggaag ccggatcgca ttatcggcgt
    12361 ggtgccaacg ctgttttgca cgccgggaat gcgcctgctg gcgaaacttt ctggtgcgcg
    12421 taccgtgctg catattcagg attacgaagt ggacgccatg ctggggctgg gccttgccgg
    12481 aaaaggcaaa ggcagcaaag tggcacagct ggcaacggcg ttcgaacgta gcggactgca
    12541 taacgtcgat aacgtctcca cgatttcgcg ttcgatgatg aataaagcca tcgaaaaagg
    12601 cgtggcggcg gaaaacgtca tcttcttccc caactggtcg gaaattgccc gttttcagca
    12661 tgttgcagat gccgatgttg atgcccttcg taaccagctt gacctgccgg ataacaaaaa
    12721 aatcattctt tactccggca atattggtga aaagcagggg ctggaaaacg ttattgaagc
    12781 tgccgatcgt ctgcgcgatg aaccgctgat ttttgccatt gtcgggcagg gcggcggcaa
    12841 agcgcggctg gaaaaaatgg cgcagcagcg tggactgcgc aacatgcaat ttttcccgct
    12901 gcaatcgtat gacgctttac ccgcactgct gaagatgggc gattgccatc tggtggtgca
    12961 aaaacgcggc gcggcagatg ccgtattgcc gtcgaaactg accaatattc tggcggtagg
    13021 cggtaacgcg gtgattaccg cggaggccca cacagaactg gggcagcttt gcgaaacctt
    13081 tccgggcatt gcggtttgcg tagaaccgga atcggtcgag gcgctggtgg cggggatccg
    13141 tcaggcgctc ctgctgccca aacacaacac ggtggcacgt gaatatgccg aacgcacact
    13201 cgataaagag aacgtgttac gtcaatttat aaatgatatt cggggataat tatggcgcag
    13261 tcgaaactct atccagttgt gatggcaggt ggctctggta gccgcttatg gccgctttcc
    13321 cgcgtacttt accccaagca gtttttatgc ctgaaaggcg atctcaccat gctgcaaacc
    13381 accatctgcc gcctgaacgg cgtggagtgc gaaagcccgg tggtgatttg caatgagcag
    13441 caccgcttta ttgtcgcgga acagctgcgt caactgaaca aacttaccga gaacattatt
    13501 ctcgaaccgg cagggcgaaa cacggcacct gccattgcgc tggcggcgct ggcggcaaca
    13561 cgtcatagcc cggagagcga cccgttaatg ctggtattgg cggcggatca tgtgattgcc
    13621 gatgaagacg cgttccgtgc cgccgtacgt aatgccatgc cgtatgccga agcgggcaag
    13681 ctggtgacct tcggcattgt gccggatcta ccagaaaccg gttatggcta tattcgtcgc
    13741 ggtgaagtgt ctgcgggtga gcaggatacg gtggcctttg aagtggcgca gtttgtcgaa
    13801 aaaccgaatc tggaaaccgc ccaggcctat gtggcaagcg gcgaatatta ctggaacagc
    13861 ggcatgttcc tgttccgcgc cggacgctat ctcgaagaac tgaaaaaata tcgtccggat
    13921 attctcgatg cctgtgaaaa agcgatgagc gccgtcgatc cggatctcga ttttattcgt
    13981 gtggatgaag aagcgtttct cgcctgcccg gaagagtcgg tggattacgc ggtcatggaa
    14041 cgtacggcag atgccgttgt ggtgccgatg gatgcgggct ggagcgatgt cggctcctgg
    14101 tcctcattat gggaaattag cgcccacacc gccgagggca acgtttgcca cggcgatgtg
    14161 attaatcaca aaactgaaaa cagctatgtg tacgccgaat ctggcctcgt aaccaccgtc
    14221 ggggtgaaag atctggtagt ggtgcagacc aaagatgcgg tgctgattgc cgaccgtaac
    14281 gcggtgcagg atgtgaaaaa agtggtcgag cagatcaaag ccgatggtcg ccatgagcat
    14341 cgggtgcatc gcgaggtcta tcgcccgtgg ggcaaatatg actctatcga cgcgggcgac
    14401 cgctaccagg tgaaacgcat caccgtgaaa ccgggcgagg gcttgtcggt acagatgcac
    14461 catcaccgcg cggaacactg ggtagtggtc gcgggaacgg caaaagtcac tattgacggt
    14521 gatatcaaac tgcttggtga aaacgagtcc atttatattc cgctgggggc gacgcactgc
    14581 ctggaaaacc cggggaaaat tccgctcgat ttaattgaag tgcgctccgg ctcttatctc
    14641 gaagaggatg atgtggtgcg cttcgcggat cgctacggac gggtgtaaac gtcgcatcag
    14701 gcgctgtttg tcggatgcgg cgtgaacgcc ttatccgacc tacggttcgg atttgtaggc
    14761 ctgataagac gcggcagcgt cgcatcaggc aatgaatgcg aaaccgagat gtaaataacg
    14821 acaaaaacaa aattggccgt ttcggtcagg gccaactatt gcctgaaaaa gggtaacgac
    14881 atgaaaaaat taacctgctt taaagcctat gatattcgcg ggaaattagg cgaagaactg
    14941 aatgaagata ttgcctggcg cattgggcgt gcctatggcg aatttctcaa accgaaaacc
    15001 attgtgttag gcggtgatgt ccgtctcacc agcgaaacct taaaactggc gctggcaaaa
    15061 ggtttacagg atgcgggcgt tgacgtgctg gatatcggta tgtccggcac cgaagagatc
    15121 tatttcgcca cgttccatct cggcgtggat ggcggcattg aagttaccgc cagccataat
    15181 ccgatggatt ataacggcat gaagctggtt cgcgaggggg ctcgcccgat cagcggagat
    15241 accggactgc gcgacgtcca gcgtctggct gaagccaacg actttcctcc cgtcgatgaa
    15301 accaaacgcg gtcgctatca gcaaatcaac ctgcgtgacg cttacgttga tcacctgttc
    15361 ggttatatca atgtcaaaaa cctcacgccg ctcaagctgg tgatcaactc cgggaacggc
    15421 gcagcgggtc cggtggtgga cgccattgaa gcccgcttta aagcccttgg cgcacccgtg
    15481 gaattaatca aagtgcacaa cacgccggac ggcaatttcc ccaacggtat tcctaacccg
    15541 ctactgccgg aatgccgcga cgacacccgc aatgcggtca tcaaacacgg cgcggatatg
    15601 ggcattgcct ttgatggcga ttttgaccgc tgcttcctgt ttgacgaaaa agggcagttt
    15661 atcgaaggct actacattgt cggcctgctg gcagaagcgt tcctcgaaaa aaatcccggc
    15721 gcgaagatca tccacgatcc acgtctctcc tggaataccg ttgatgtggt gactgccgct
    15781 ggcggcacgc cggtgatgtc gaaaaccgga cacgccttta ttaaagaacg tatgcgcaag
    15841 gaagacgcca tctacggtgg cgaaatgagc gctcaccatt acttccgtga tttcgcttac
    15901 tgcgacagcg gcatgatccc gtggctgctg gtcgccgaac tggtgtgcct gaaaggaaaa
    15961 acgctgggcg aactggtgcg cgaccggatg gctgcgtttc cggcaagcgg tgagatcaac
    16021 agcaaactgg cgcaacccgt tgaggcgatt aatcgcgtcg aacagcattt tagccgtgag
    16081 gcgctggcgg tggatcgtac cgatggcatc agcatgacct ttgccgactg gcgctttaac
    16141 ctgcgcacct ccaacaccga accggtggtg cggttgaatg tggaatcgcg cggtgatgtg
    16201 ccgctgatgg aagcgcgaac gcgaactctg ctgacgttgc tgaatgagta atgtcggatc
    16261 ttcccttacc ccactgcggg taaggggcta ataacaggaa caacgatgac aaatctaaaa
    16321 aagcgcgagc gagcgaaaac caatgcatcg ttaatctcta tggtgcaacg cttttcagat
    16381 atcaccatca tgtttgccgg actatggctg gtttgcgaag tcagcggact gtcattcctc
    16441 tacatgcacc tgttggtggc gctgattacg ctggtggtgt tccagatgct gggcggcatc
    16501 accgattttt atcgctcatg gcgcggtgtt cgggcagcga cagaatttgc cctgttgcta
    16561 caaaactgga ccttaagcat gattttcagc gccggactgg tggcgttcaa caatgatttc
    16621 gacacgcaac tgaaaatctg gctggcgtgg tatgggctga ccagtatcgg actggtggtt
    16681 tgccgttcat gtattcgtat tggggcgggc tggctgcgta atcatggcta taacaagcgc
    16741 atggtcgccg tagcggggga tttagccgcc ggacaaatgc tgatggagag cttccgtaat
    16801 cagccgtggt tagggtttga agtggtgggc gtttaccacg acccgaaacc gggcggcgtt
    16861 tctaacgact gggcgggcaa tctgcaacag ctggtcgagg atgctaaagc gggcaagatt
    16921 cataacgtct atatcgcgat gcaaatgtgc gacggcgcgc gagtgaaaaa actggtccat
    16981 caactggcgg acaccacctg ttcggtgctg ctgatccccg atgtctttac cttcaatatt
    17041 ctccattcac gtctcgaaga gatgaacggc gttccggtgg tgccgctgta tgacacgccg
    17101 ctttccgggg ttaaccgcct gctaaaacgt gcggaagaca ttgtgctggc gacgcttatc
    17161 ctgctgctga tctccccggt gctgtgctgc attgcgctgg cggtgaaact cagttcacct
    17221 gggccggtta ttttccgcca gactcgctac ggcatggatg gcaagccgat caaagtgtgg
    17281 aagttccgtt ccatgaaagt gatggagaac gacaaagtgg tgacccaggc gacgcagaac
    17341 gatccgcgcg tcactaaagt ggggaacttt ctgcgccgca cctcgctgga cgaattgccg
    17401 cagtttatca atgtgctgac cggggggatg tcgattgtcg gtccacgtcc acacgcggtg
    17461 gcgcataacg aacagtatcg ccagctcatt gaaggctaca tgctgcgtca taaagtgaaa
    17521 cccggcatta ccggctgggc gcagattaat ggctggcgcg gcgaaaccga cacgctggag
    17581 aaaatggaaa aacgcgtcga gttcgacctt gagtacatcc gcgaatggag cgtctggttc
    17641 gatatcaaaa tcgttttcct gacggtattc aaaggcttcg ttaacaaagc ggcatattga
    17701 tatgagctta cgtgaaaaaa ccatcagcgg cgcgaagtgg tcggcgatag ccacggtgat
    17761 catcatcggc ctcgggctgg tgcagatgac cgtgctggcg cggattatcg acaaccacca
    17821 gttcggtttg cttaccgtgt cgctggtgat catcgcgctg gcagatacgc tttctgactt
    17881 cggtatcgct aactcgatta ttcagcgaaa agaaatcagc caccttgaac tcaccacgct
    17941 gtactggctg aacgtcgggc tggggcttgt ggtgtgcgtg gcggtgtttt tgttgagtga
    18001 tgccattggc gacgtgctga ataacccgga cctggcaccg ttgattaaaa cattgtcgct
    18061 ggcgtttgtg gtgatccccc acgggcaaca gttccgcgcg ttgatgcaga aggagctgga
    18121 gttcaacaaa atcggcatga tcgaaaccag cgcggtactg gcgggcttca cttttacggt
    18181 ggttagcgcc catttctggc cgctggcgat gaccgcgatc ctcggttatc tggtcaatag
    18241 tgcagtaaga acgctgctgt ttggttactt tggccgcaaa atttatcgcc ccggtctgca
    18301 tttctcgctg gcgtcggtgg caccgaactt acgttttggt gcctggctga cggcggacag
    18361 catcatcaac tatctcaata ccaacctttc aacgctggtg ctggcgcgta ttctcggcgc
    18421 gggcgtggca gggggataca acctggcgta caacgtggcc gttgtgccgc cgatgaagct
    18481 gaacccaatc attacccgcg tgttgtttcc ggcatttgcc aaaattcagg acgacaccga
    18541 aaagctgcgc gttaacttct acaagctgtt gtcggtagtg gggattatca actttccggc
    18601 gctgctgggg ctgatggtgg tgtcgaataa ctttgtaccg ctggtctttg gtgagaagtg
    18661 gaacagcatt attccggtgc tgcaattgct gtgtgtggtg ggtctgctgc gctccgtagg
    18721 taacccgatt ggttcgctgc tgatggcgaa agcgcgggtc gatatcagct ttaaattcaa
    18781 cgtattcaaa acatttctgt ttattccggc gattgttata ggtgggcaga tggcgggcgc
    18841 gatcggcgtc acgcttggct tcctgctggt gcaaattatc aacaccattc tgagttactt
    18901 cgtgatgatt aaaccggttc ttggttccag ttatcgtcag tacatcctga gtttgtggct
    18961 gccgttttac ctctcgctgc cgacgctggg ggtcagttat gcgctgggca ttgtgctgaa
    19021 agggcaattg gcgctgggga tgctgctggc ggtgcaaata gccgcgggcg tgctggcgtt
    19081 tgtggtgatg attgtgctgt cgcgccatcc gctggtggtg gaagtgaagc gtcagttttg
    19141 tcgcagcgaa aaaatgaaaa tgcttttacg ggcggggtga gttgctgttt tcggttccga
    19201 caacggttgc cggatgcggc gtaaaacgcc ttatccgtcc tacggttctg tgctcgggac
    19261 ctgtaggcct gataagacgc gtcagcgtcg catcaggcac tgaatgccgg atgcggcgtg
    19321 aacgccttat ccggcctacg gttcagggct tggatcttgt aggcc
//
LOCUS       DAZFXT010000087        19148 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.087, whole genome shotgun sequence.
ACCESSION   DAZFXT010000087 DAZFXT010000000
VERSION     DAZFXT010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.087"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            42..235
                     /locus_tag="ACHKW0_003312"
                     /pseudo
     CDS             42..235
                     /locus_tag="ACHKW0_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            345..608
                     /locus_tag="ACHKW0_003313"
                     /pseudo
     CDS             345..608
                     /locus_tag="ACHKW0_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001165851.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
     gene            832..957
                     /locus_tag="ACHKW0_003314"
                     /pseudo
     CDS             832..957
                     /locus_tag="ACHKW0_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
     gene            complement(1017..1523)
                     /gene="yciE"
                     /locus_tag="ACHKW0_003315"
     CDS             complement(1017..1523)
                     /gene="yciE"
                     /locus_tag="ACHKW0_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7476099.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKIAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(1569..2069)
                     /gene="yciF"
                     /locus_tag="ACHKW0_003316"
     CDS             complement(1569..2069)
                     /gene="yciF"
                     /locus_tag="ACHKW0_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HFO7476100.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(2155..2334)
                     /gene="yciG"
                     /locus_tag="ACHKW0_003317"
     CDS             complement(2155..2334)
                     /gene="yciG"
                     /locus_tag="ACHKW0_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HFO7476101.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            complement(2715..3521)
                     /gene="trpA"
                     /locus_tag="ACHKW0_003318"
     CDS             complement(2715..3521)
                     /gene="trpA"
                     /locus_tag="ACHKW0_003318"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HFO7476102.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(3521..4714)
                     /gene="trpB"
                     /locus_tag="ACHKW0_003319"
     CDS             complement(3521..4714)
                     /gene="trpB"
                     /locus_tag="ACHKW0_003319"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HFO7476103.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     AREEI"
     gene            complement(4726..6087)
                     /gene="trpCF"
                     /locus_tag="ACHKW0_003320"
     CDS             complement(4726..6087)
                     /gene="trpCF"
                     /locus_tag="ACHKW0_003320"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HFO7476104.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(6088..7683)
                     /gene="trpD"
                     /locus_tag="ACHKW0_003321"
     CDS             complement(6088..7683)
                     /gene="trpD"
                     /locus_tag="ACHKW0_003321"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HFO7476105.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(7683..9245)
                     /gene="trpE"
                     /locus_tag="ACHKW0_003322"
     CDS             complement(7683..9245)
                     /gene="trpE"
                     /locus_tag="ACHKW0_003322"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HFO7476106.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(9267..9360)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(9337..9381)
                     /gene="trpL"
                     /locus_tag="ACHKW0_003323"
     CDS             complement(9337..9381)
                     /gene="trpL"
                     /locus_tag="ACHKW0_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HFO7476107.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            9519..10400
                     /gene="rnm"
                     /locus_tag="ACHKW0_003324"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
     CDS             9519..10400
                     /gene="rnm"
                     /locus_tag="ACHKW0_003324"
                     /gene_synonym="trpH"
                     /gene_synonym="yciV"
                     /EC_number="3.1.13.-"
                     /EC_number="3.1.3.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase AM"
                     /protein_id="HFO7476108.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            10397..11017
                     /gene="yciO"
                     /locus_tag="ACHKW0_003325"
     CDS             10397..11017
                     /gene="yciO"
                     /locus_tag="ACHKW0_003325"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HFO7476109.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            11118..11990
                     /gene="rluB"
                     /locus_tag="ACHKW0_003326"
     CDS             11118..11990
                     /gene="rluB"
                     /locus_tag="ACHKW0_003326"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HFO7476110.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(12030..12620)
                     /gene="cobO"
                     /locus_tag="ACHKW0_003327"
     CDS             complement(12030..12620)
                     /gene="cobO"
                     /locus_tag="ACHKW0_003327"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HFO7476111.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(12617..13375)
                     /gene="yciK"
                     /locus_tag="ACHKW0_003328"
     CDS             complement(12617..13375)
                     /gene="yciK"
                     /locus_tag="ACHKW0_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HFO7476112.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRITVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVIQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            13595..14644
                     /gene="sohB"
                     /locus_tag="ACHKW0_003329"
     CDS             13595..14644
                     /gene="sohB"
                     /locus_tag="ACHKW0_003329"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HFO7476113.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(14680..14931)
                     /gene="yciN"
                     /locus_tag="ACHKW0_003330"
     CDS             complement(14680..14931)
                     /gene="yciN"
                     /locus_tag="ACHKW0_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HFO7476114.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            15311..17908
                     /gene="topA"
                     /locus_tag="ACHKW0_003331"
     CDS             15311..17908
                     /gene="topA"
                     /locus_tag="ACHKW0_003331"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HFO7476115.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGGALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            18118..19092
                     /gene="cysB"
                     /locus_tag="ACHKW0_003332"
     CDS             18118..19092
                     /gene="cysB"
                     /locus_tag="ACHKW0_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HFO7476116.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4717 a         4732 c         4967 g         4732 t
ORIGIN      
        1 aatagctata tgcgtacatt ctcaacctgc ccgtcacgta gctggttgaa tcactttttc
       61 ttgataaagg gctggtcctg ccaccgttgt tgttgggtgc gttaaaaata cactactaat
      121 atgcccggca tgcgggcaac agagtcctgt tcacgatcgc cgccaccgga aatggcgctg
      181 cctcgatacc tgccagttca tggcactatt cgaagccgat gttccccacg ttatatggag
      241 taatcattgg ggttatgccg cagcacgttg tatgcaagaa tgtgctgcgg ctggtttgtg
      301 agctttcgat agtggttgtt atttttgccc ttatttgttc cggaggccct ggttcaatgg
      361 tccgtctgcc ccctgtggtg atgtcagcaa aatcagccac tgcgcgaacc acaatagccc
      421 gggaagatgc tgaagatcac caggtaaagc tgtcagcgca gaaactggaa gaactgctcg
      481 catcaatggt taaggatgag gttgatcgca atgatgggat ttattgacgt cagcgagagc
      541 agaaggaaga actgaataac ctgaatgatt tacgctcaat cagagcgatg atgattggca
      601 gtaattagcg gtcaatttcg ggttttttgg ctgtgcgctc cccgcccagc attgcgcgtc
      661 aattagcccc aaaaaaaacg aaaaagttac aatcaagtaa aaaattcctt ctgcaaaact
      721 aaaaaagatt tcaataaaat caggcagtta aaaacataaa aaaaaggtac aaaaaagcat
      781 ctcaaagtct tgttttttgc ttcaaataca attggttatt gtgttttttg aaacctacca
      841 gtgtggtaca tggatatcga taccaccgcc aattataagc taggcggtgc acagcaacac
      901 gatagcgtac gcctcgatcc gtgggtgttt atgttctcag caggatatcg tttttaattc
      961 cacaaaaaaa cgaccccgta atatacgggg tcaataagga catggcataa agtgtattat
     1021 ttcttcgctt ctacgccgtc agtttcagag cgaattaaaa atttctcagt tgtttgcgga
     1081 atatgctgaa tcagccagtc ggccatttgc ttttcctcat ttaaaatcgc ttcgatggtt
     1141 ggaattgaag ctgtatcacc ggcatttttt gctgctgcta atagtgaggt gtaacaggcg
     1201 atttcaaatt gctcgaagac atatccgcta atagagcctt tgactatttc atcagaaggg
     1261 aatataccac cgattgactg cccaagcgca gctattttac tcatggaatc tttaatgact
     1321 gaacgtgaaa tgtcattacg atcaagaata gtttccagtt gaacaatctg gtttttggtt
     1381 tcactaagat gttgttcaat acgagcgcgt agttcaggat aattatctat acggctggcc
     1441 atggattcaa gcatagattc ggcttgcttt tccattgcgt gggcgtcacg taaccagtca
     1501 tgataatgtt caatacgatt catgaaaatg tcctctttat aaaatgacta aaagttaaag
     1561 ttcatatttc aggctttatt ttcggctttc ttatttacgt tattaagggc cagatcagtc
     1621 agtttgatgt cggtggcctt ttcttcttcc agggtctctt tcagaagctt cgctgcttta
     1681 cgataaccta attgttcagc cagcgtcgct aatgtcccgt aactggcaat ctcataatgc
     1741 tcgactttct gtgctgcggc aatcagtgcg gcatcacgca cttcgttttt ctcggtactt
     1801 tcgatgacct cattagcttc ttcaataaga ccttccattg ccacacattt catgcgctta
     1861 attttcagat tcgattctga ttccacaact tgatcaatac gttcaatctg tccatgtgtt
     1921 tcctcgaggt gcgcatgaaa agcctgactt aatttttcgt ttgatgttgc tcttgcgagt
     1981 tttgccagtg cccgggttaa ttgtttttct gcgctgtagg tatctgaaag caggtgaata
     2041 aatacatctt caatggtctt catattcata tttttctcca gtgaaatcac tgcgagccat
     2101 acggcccgca gatgcaatta tctttatcag ctgatattaa taattaacgg tgaatcagga
     2161 tttgcctgat ttattaccac cgctttgttg accgcctttt ttacccgctt cagatgcgcg
     2221 ttgcggatca tttttaaaat taccgccgct atgctgaccg cctttacggc ctgcgtcgga
     2281 tgccttctca cggtcttcgg cgaaatttcc tgaaccacca cgatgttcgg ccatgttatt
     2341 tctcccgttg cgttgcattg tttcattaat atgagtgttg tgtgtcgaca ctcattaaaa
     2401 ttagtcgcta atgagaatta gtcaaattaa gcgcaacgag aagatagagg gaaaatatat
     2461 tttgaggaac attctggata tattaacaat tacctgagga ataagtgact tagagaaaat
     2521 tattgatttt actggtgtta tgttgcggga ttaatttgtt tgattatatt actgttgggc
     2581 ggaaaaatga cgtaagttga cgttcgaccg gggtaagcga aacggtaaaa agataaatat
     2641 taaatgaatt taggattttt ccggcttcat taaagaaagt taaaatgccg ccagcggaac
     2701 tggcggctgt gggattaact gcgcgtcgcc gctttcatcg gttgtacaaa aactttcagt
     2761 gccgccagca ttttctctgg ctcattaata tgttgctcga tgattttaac aatggccgaa
     2821 ccagaaatcg cgcccgcagc tcctgcatca atcgctgctt ttacctgatc cggggcggaa
     2881 ataccaaacc cctgcaatgg aggtgcagcg ttgtactctt tcagcttcgc aaccagatga
     2941 ttgaggggta acgcggcgcg gttttctgcg ccggtcacgc ccgctcgtga cagcaaatag
     3001 gtgtaaccac gaccgtaaga ggctatctgg cgcagcaggt cgtcatcggc atttggcggg
     3061 cagatgaaga taggtgcgac attatggcgc aacgcggcct ggcggaaggg cgcggactct
     3121 tcaattggca catcggcaac cagcaccgaa tcgacgccga ctttctcgca ctgggcataa
     3181 aactcatcaa tgcctttgtt aaacaccaga ttggcataca tcaacaggcc aatgggaatg
     3241 gtcgggtgtt tctggcgaat cagtgccagc atttcaaaac attgtgccgg agtcacacct
     3301 gccgcaaagg cgcgcagagt ggcgttttga atggtcgggc catccgctag agggtcggag
     3361 aaggggatac ctaactccag cgcgtcagca ccggcttcaa ttagcgtatc gataattttc
     3421 aatgactgct caatgcccgg atcaccgagc gtgacgaaag gaacgaatgc gccttctttg
     3481 cgctccttca actgggcaaa cagagattcg tagcgttcca tcagatttcc tctcgtgctt
     3541 tcaaaatatc gtgaacggtg aagatgtctt tatcgccgcg accggaaagg ttaaccacca
     3601 gtagctgctc tttatccggg ttttcgcgca tcattttcaa cgcatgggcc agggcgtggg
     3661 aggattccag cgccgggatg atcccttcgt gcaggcacag cgttttgaag gcttcaaggg
     3721 cttcatcatc ggtaatagac acgtaatcag cgcgtccagt gctgttaaga tacgcgtgtt
     3781 gtgggccgac agacgggaaa tccagtccgg cggagatgga gtaagattct tcaatctgcc
     3841 cgtcttcggt ttgcatcatc ggcgctttca taccgaaata gatgcccacg cgaccatgtt
     3901 ttaacggtgc gccgtgctcg ccagtttcga taccgtgacc acctggctcc acaccaatca
     3961 ggccgacgtt ggtttcattg atgaaatcag caaacatgcc gatggcattc gaaccgccgc
     4021 caacacaggc gataacggca tccggcaggc gaccttctct ttccagaatc tgcgctttgg
     4081 tttcttcgcc aatcatccgc tgaaactcac gcacaatggt cggataagga tgcgggccag
     4141 ctgcggtgcc cagcatatag tgcgcggttt cgtaactacc ggaccagtcg cgcagcgcct
     4201 cgttacaggc atctttcagc gtcgcggaac cgctatgcac cgggatcact tccgcaccca
     4261 ttaagcgcat acgaaaaacg ttaggcgact ggcgttctac gtctttggca cccatataaa
     4321 tacggcattt caggccgagc agggcgctgg caagggccga cgccacgcca tgctgaccgg
     4381 caccggtttc ggcgatgatt tcggttttac ccatccgctt cgccagtaaa gcctgaccga
     4441 gcacctggtt agttttatgc gcgccgccgt gcagcaaatc ttcacgcttg agatacagag
     4501 tggtgttcgt cccggctgta atgttctggc atttggtcag cgcggttgga cgcccggcat
     4561 agtttttcag caggtcgttg aactgagcct gaaattcagg atctttttgc gcactgacaa
     4621 aagcttcttc cagctggcgc agagcaggca tcaggatttg tggcacgtac atgccgccaa
     4681 actcaccaaa ataggggtta agtaatgttg tcattgttcc tttccttaat atgcgcgcag
     4741 cgtctggaaa accgaggcca aaagacgtgc gtctttgatg cccggttgcg actctacagc
     4801 agaattaaaa tcaagtccgg cgcagccggt ttgtgccgct tccacgcagt tatctgcgcc
     4861 taagcccccc gccagcagaa cgttgccaag cgattgacca tttaatagtg accagtcgaa
     4921 acgttgcccg cttccaccct ggccgttgtc taaaacatat ttatcaacgt gctgaagctc
     4981 gcgggcgggc agggtttcac cgacgcttaa tgctttccag atggcaacgt gtgctggcag
     5041 agcttcacgc agcgtatcga tatacagctg atcttcatta ccatgcagtt gcactgccgc
     5101 cagcgataac accttagctt tgtccaccac atcggcaata tcgtgattgc ggaacacgcc
     5161 aacatactgc aacggtgccg cagccatcac ttcctgcgcc tgttcaacgt tgacgcaacg
     5221 cggtgatgtc gcaacaaaaa tcaacccacc gtaaatcgcg cccgcgtcat aagctgcttt
     5281 agcgtcttgc ccacgcgtca ggccacatac tttattctca cccagcaaca cccggcgcac
     5341 ggcggcgtgc aaatcgtcat gggccatcaa cgccgaacca atcagaaaac cgttagcgaa
     5401 gtggcttaac tcgcgcacct gagcgtaagt attgatgccg gattcgctga ttaccgttac
     5461 gttgtgcccc agtttcggcg caagctcgcg ggtacggttg agatcaatcg acaaatcacg
     5521 cagatcgcgg ttgttgatgc caacgacctt tgcccccaat gcaatggcac gctccagttc
     5581 ctcttcatta ctgacttcgg tcagcacacc catctccaga ctgtgggcga cggcggcaag
     5641 ctggcgatat tgttcgtcat ccagtactga aagcattaat aagcaggcat cagcctggta
     5701 atagcgcgcc agatagatct ggtaaggatc gataatgaag tctttacata aaatcggctg
     5761 cggggcgatt tggctgacga tggggaggaa atcaaagctc ccctgaaaat atttctcatc
     5821 agtcagcacc gaaattgccg aagcgtaatg tttataaatg gcggcaatgc gtgctggatc
     5881 gaaatcatca cggatcacgc cttttgacgg cgacgctttc ttgcactcca gaataaacgc
     5941 cgtgcgcgca ccctgtagcg catcataaaa atgtcgcgtg ctcggctgaa cctcattctg
     6001 aaaactggcc agcggttgct gctgtttgcg ggtttctacc caaatcgcct tgtctgcgac
     6061 gattttcgct aaaacggttt gcatcattta ccctcgtgcc gccagtgcgg taactctgtc
     6121 gtaagcggaa ccactgcgca gtacctcaag aacggtttgc gcattggctt gcagatcttc
     6181 atggccatgc aggcgcatta acatggcgac gttcgcagcg acggctgctt catgggcggc
     6241 gtcgccttta ccttgtaaca agcgtgttaa aatgtcacgg ttttcttccg gtgttccgcc
     6301 tgccagttgc tcctggtggt agggagtcag gccaaaatct tcagcggtca attgatagct
     6361 cttaatttcg ccgtcatgca gctcggcaac gattgtcggc gcgtgtaatg aaacttcatc
     6421 catcccgccg ctgtgcacca ccgccgcgcg ttgatacccc agcacgcgca aggtttcggc
     6481 aatcggcagc accagttccg gactataaac accaattaac gccagcggcg gatgcgccgg
     6541 gttaatcaat ggccccagca cattgaacag ggtgcgggtt ttcagttgct ggcgaaccgg
     6601 catcgcgtgg cggaatccgg tgtgatactt cggcgcaaag aggaaacata cacctaactc
     6661 atccagcgcc tggcgcgatt tatcggcgtt catatcaaga ttaataccga acgccgccag
     6721 cagatcggac gaaccagatt tactggagac gctacggttg ccgtgtttcg ccactttcag
     6781 cccacaggcc gcggcgacaa acgcactggc ggtagaaata ttgatactgt tgctgccgtc
     6841 accgccagta ccgacgatat cagcaaacag ataatccggg cgcgggaacg gcgcggcgtt
     6901 ttccagtagc gcggttgctg ctccggcgat ctcgttcggg tgctcaccgc gaattttcat
     6961 gctcaccagc gccgccgcca gttgttccgg cttcagctcg ccacgcacca ccgctgaaaa
     7021 cagctggtgg ctttcttgtt ggctaagcgt ctgcgcctga tacagttttt ccagaatcgg
     7081 ttgcagcgtg ttggctggct ctagtttctg ctgcgcccag gccagcgttt gttccagcag
     7141 gcgagcgccc tgggtggtga gaatggattc cggatggaac tggaatccac aaacgcgatc
     7201 cgcatcgtga cgtactgcca tcaccatgcc attaaaatgg gcgttgatgg ttaaaccggc
     7261 cggaatgtta ctgccaacca gcgagtgata acgcgccacc ggcagcgggt ttgttaatcc
     7321 ggcaaacatc gcctgaccgt catgttcaat gctcgacgct ttaccgtgaa gaatttcgcc
     7381 cgcctgaccg acatagcccc cgtaagcttc gacaattgcc tgatgtccga ggcaaatgcc
     7441 aataatgggc agcttgccac gcaagcgggt gaggagttcc ggcatacaac cggcttcgct
     7501 cggcacaccg gggccaggag aaagcatcag taccggattg ctcatcgtcg ccaggcgttc
     7561 aattaaggtc tgcgccggaa tatggttgcg gtaaatcacc acattatgcc cattgctgcg
     7621 caactgatct gccaggttgt acgtaaaaga gtcgatatta tcgagcagca gaatgtcagc
     7681 catcagaaag tctcctgtgc atgatgcgcg gtggcaatag cgcgcagtac agcgcgggct
     7741 ttattacggg tttcgtcggc ttccgactgc ggaacagaat caaggactac gccagcaccg
     7801 gcttgcacgg tggcgatacc gttttccacc agcgccgagc ggatcacaat gcaggtgtcg
     7861 agatcgccat gcgcggtgaa ataacctacc gcgccgccgt agctgccgcg gcggcgacct
     7921 tccgcctcgg caattaactg catagcgcgt actttcggcg caccacttaa cgttcccata
     7981 ttcatacagg cgcggtaagc gtgcagggcg tcgagatcgt ggcgcagctc accgaccacg
     8041 cgggagacca agtgcatcac gtaagagtaa cggtcaactt tggtgaggtc ggcgacgtag
     8101 cggctgccgg gggtgcaaat gcgtgccaga tcattacggg cgagatccac cagcatcaga
     8161 tgttcagaaa gctctttatg atcggtacgc atttccagtt cgatgcggct gtcgaggtct
     8221 ctgtccagcg agccatcggc acgacgaccg cgtggacgtg ttccggcaat cgggtagatc
     8281 tcaatctggc ggctggtggc gtcatacttg agcgaacttt ccggcgacgc gccaaacagg
     8341 gtgaaatcat tatcctgcat aaaaaacatg tacgggctgg gattactctt tttcagcacg
     8401 taataggccg ccagcggtga cgggcagggc agagagaaac ggcgagatgg caccacctgg
     8461 aaaatttctc cagcgcgaat cgctttttgc aacaaacgca ctacgccacc gaactcttca
     8521 tcgctctgat tacattcaca acgcatatgc ggcacggaaa ccactggcag cggcggcgcg
     8581 gcttcggtca gttgctgacg tagttcgttc aggcgagcag tgagacgttg tttttcttct
     8641 tcattcggag caaacaggct ggcctgaata cgggtgcttt ttttctgatg gtcaatcacc
     8701 atcagagttt cagcgagata aaaacagaaa tcagggcagt tattttccgc tgacagttgc
     8761 ggtaaatctt caaatcccgc cacaaggtca taagagaaca ggccgccgaa gaacattgct
     8821 tctcgttctt ccttcggtac attcaacaga ttctgcaata aacggaaagc gtcaaaaacc
     8881 gaaagggagc ataagcgagc gtcttcatcc agcagtggac tgacaggggg gaagcgcagc
     8941 acacggcagt ttggtaattg ttcattttcc acacccgcag gcagggcgtt atccagtagt
     9001 gtcagcaggg cttcgccgtt gccggaaagt gcctggattg tgacagtgtc acctaaagct
     9061 gtaatgcgca gcgcactgtc taccagcagc aggcttttta aatcatcttt gctgtcgata
     9121 tctgcggatt ccagcagcag cgttgccgga cgatccccac acaactggtg aaaaagcgcg
     9181 gtgggattgt cgcgataagc gccttcgcag gttagcagtt cgagagtcgg tttttgtgtt
     9241 tgcattgtta ttctctaatt ttgttcaaaa aaaagcccgc tcattaggcg ggctgggtat
     9301 ctgattgctt tacgcatggt gaatacactg cccgtttcag gaagtgcgcc accaaccttt
     9361 cagtacgaaa attgctttca ttgtcgatac cctttttacg tgaacttgcg tactagttaa
     9421 ctagttcgat gattaattgt caacagctca tttcagaata tttgccagaa ccgttatgat
     9481 gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg aattatgcag
     9541 tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg ccagaagcat
     9601 tggtgcaccg tgcagtcgag atgcgcgtcg gcaccctggc gatcaccgac catgacacca
     9661 cagctgcaat cgcgccagca agagaggaaa tttcacgttc gggactggcg ttgaatctta
     9721 ttcccggcgt ggaaatttcc acggtctggg aaaatcatga aattcatatt gtcgggctga
     9781 atattgatat tactcatccg ctaatgtgtg agttcctcgc gcagcagaca gaacggcgca
     9841 atcagcgggc gcagctgatt gccgaaagac ttgaaaaagc gcaaatacct ggggcgctgg
     9901 aaggcgcaca acgactggcg cagggcggcg cagtgacgcg cggtcatttt gcgcgtttcc
     9961 ttgttgagtg cggcaaagcc agttcaatgg cggatgtctt taaaaagtat ctggcgcgcg
    10021 ggaaaaccgg atacgttccg ccacagtggt gtacaataga acaagctatt gatgtcattc
    10081 atcattctgg cggtaaggcg gtgctagctc atcctgggcg gtacaatctt agtgctaaat
    10141 ggctgaaaag attggttgcg cattttgccg aacaccacgg tgacgcgatg gaagtcgcgc
    10201 agtgccagca atcgcccaat gaacgtaccc agctggcggc ccttgcgcgt cagcatcatt
    10261 tatgggcatc acaaggatct gattttcatc agccatgccc gtggatcgaa ctgggtcgta
    10321 aactctggct gcccgcaggc gttgaaggcg tctggcagct atgggaacag ccgcagaaca
    10381 ccacagagag ggaattatga gccagttttt ttatattcat cctgataacc cacagcaacg
    10441 tctgatcaac caggcggtgg agatcgtgcg taaaggcggg gtgattgttt atccaactga
    10501 ttccggctat gcgctcggct gtaaaattga agataaaaac gcgatggagc gtatttgtcg
    10561 tattcgccag ctgccggacg gtcacaactt taccctgatg tgtcgcgatc tttctgaact
    10621 ctcgacctat tcatttgttg ataacgttgc gtttcgttta atgaaaaaca acacgccggg
    10681 caactatacc ttcatcttga aggggacgaa ggaagtgcca cgccgtctgt tgcaggaaaa
    10741 acgcaaaacc atcggtatgc gtgtgccttc taacccgatt gcgcaggcgt tgcttgaagc
    10801 actgggcgaa ccgatgcttt ccacttcgct aatgctgcca ggcagcgaat ttaccgaatc
    10861 cgatcccgaa gaaattaaag accgtctgga aaaacaggtg gatttgatta tccacggcgg
    10921 ctatctcgga cagaaaccga caacggttat tgatcttacc gatgatacgc cggtcgtggt
    10981 gcgtgaaggc gtaggtgatg tgaagccttt cttataagtg cgtaactctg tatactacgc
    11041 ggccttaaaa tggcgctata cggcgccatt gattctcctg ttcgacgcct gtgaaggcga
    11101 caccaaaagg aagctctatg agcgaaaaat tacagaaagt gctggcccgc gctggccacg
    11161 gctcccgccg tgaaatcgaa tctattattg aagccggtcg tgtgagtgtt gatggcaaaa
    11221 ttgctaaact cggcgatcgc gttgaagtta cccctgggct gaaaattcgt atcgatggtc
    11281 atctgatttc ggtacgtgag tccgctgaac aaatttgtcg cgttctggcc tattacaaac
    11341 cggaaggtga gttgtgtacc cgtaacgatc cggaagggcg tccgacggtg tttgaccgtc
    11401 tgccaaagct gcgtggcgca cgctggattg ccgtaggtcg tctggacgtt aacacctgtg
    11461 gtctgctgct gttcaccacc gatggtgaac tggcaaaccg tttaatgcat ccaagccgtg
    11521 aagttgaacg tgaatacgcc gtgcgtgtat ttggtcaggt tgacgacgcg aaactgcgtg
    11581 atttgagtcg tggcgtgcaa ctggaagatg gtccggcagc ttttaaaacc atcaagttca
    11641 gcggcggcga agggatcaac cagtggtaca acgtgactct gaccgaaggc cgtaaccgtg
    11701 aagttcgtcg tctgtgggaa gcggttggtg tgcaggtgag ccgcctgatc cgtgttcgtt
    11761 acggtgatat cccactgcca aaaggtctgc cacgcggtgg ctggacggag ctggacctcg
    11821 cccagactaa ctatctgcgc gaactggtgg agcttccgcc agaaaccagc tctaaagtcg
    11881 ctgtagaaaa agaccgtcgt cgcatgaagg ctaatcagat tcgtcgtgcg gtgaaacgtc
    11941 acagtcaggt gagcggcggt cgtcgttctg gcgggcgtaa taacggttaa tcaaagtatg
    12001 ccggacgtca tatccggcat ttttacagat taataatcaa tccctatttg cgccttaaca
    12061 ccggcatcaa acgcatgttt gatggggcgc agctcactta ccgtatccgc cagttcaaga
    12121 atatcccgat gacagcctcg acccgtgata atcaccgtct gttgatgtgg acgttcattt
    12181 aacgcctgca ccacttcttc cagtggcaaa tagtcatacg ccaccatata cgtcagttca
    12241 tcaagcaaaa ccatatccag tgaggaatca gcaagcatcc gctttgcatg ttgccagact
    12301 tcgcggcagg cggcggtatc agactcgcgg ttttgtgtat cccaggtaaa gcccgttgcc
    12361 atcacctgaa actcaacgcc atgtggctcc agcagattgc gttcgccatt aggccaggtg
    12421 cctttaataa actgcacgac gcctactttt tttccgtgac caactgcgcg tgttgccgta
    12481 ccaaatgccg cggtggtttt gccttttcca ttgccggtaa agacgatgat aataccgcgt
    12541 tcatcctggg cctgggccac acgagcatct actttttctt tcactcgctg ctgacgctgt
    12601 tggtagcgtt catcactcat tgggaaattc ctggtttacg gcccggttgg gcgtcaaagg
    12661 tcatgccggt tttacggcgg ctgtcatcgc ccatcagcca gaggtagagc ggcatgatat
    12721 cagcgggtgt tttaagtttc tgtggatctt cggtcgggaa ggcgctggca cgcattgcgg
    12781 tgcgcgtacc gcctgggtta atgcagttga cgcgcaggcg ctgctgatat tcatcggcca
    12841 gtacctgcat catcccttcg gtggcaaatt tcgacgctgc atatgcgccc cagttggctc
    12901 gtccctgacg tccaacgctt gatgaagtaa agaccagtga accggcgtcc gatttgagta
    12961 ataaaggaag cagtgcctgg gtgagcataa aggtggcatt aacgttgacc tgtatgacgt
    13021 cctgccagac ctgtggattt tgttcgctca ttgggcaaac atcgccgagc aatccggcat
    13081 tatgcaaaac accatccaga cgcggataat taacggtaat gcgctgtgcc agttgttggc
    13141 aatcttcgga cgtgcaggtc agcaaatcga gaataaacca ctgtggctga cgtccagttt
    13201 cttcgtttat gtggctggct acctgacgta atttttcttc attacggccc aacagaatca
    13261 ctgtcgcacc atagcgtgca tacgtcatcg cggcttcacg gccaatccca tcgctggctc
    13321 ccgtcaccag gataatgcga tcattgagta aatcttgttt tggctggtaa tgcatggcta
    13381 ctcctcaacg acgttgtctg tcgtgctgcc agtgtacttt atgactttgg ggctttatgc
    13441 ctgaaacaga agggcatttc aatcagcctg gggcgataac accgcaaaat ggatactttg
    13501 tcatactttc gctgcaataa catctctgcg agacggctta acatgcctgt tgtaaactgt
    13561 gagccaaagc gttgtttaac caaggtgggg actcgtggaa ttgttgtctg aatatggttt
    13621 gtttttggcg aaaatcgtta ccgttgtgct agcgattgcg gcgattgccg ccattattgt
    13681 caatgttgct caacgtaata aacgccagcg tggcgagtta cgggtcaaca atctcagcga
    13741 acagtataag gagatgaaag aagaactggc cgcggcgctg atggactcac atcagcaaaa
    13801 acagtggcac aaagcgcaga agaaaaagca caagcaagaa gcgaaagcag caaaagcgaa
    13861 agccaaactg ggcgaggtgg caactgacag taaacctcgc gtctgggtgc tggattttaa
    13921 aggcagcatg gacgcccatg aagtgaactc gctacgtgaa gagataacgg cggtactcgc
    13981 agcattcaaa ccgcaggatc aggttgtgct ccgtctggaa agccctggtg gcatggtgca
    14041 tggttacggg ttggcggctt cgcagctgca gcgtctgcgt gataaaaaca ttcctttaac
    14101 tgttacggta gacaaagtcg ctgccagcgg cggttacatg atggcctgtg tggcggacaa
    14161 aattgtttcc gcaccgtttg ctattgtggg ttccattggg gtggtggcgc aaatgcccaa
    14221 ctttaaccgc ttcctgaaaa gcaaagatat tgatatcgaa ctgcacactg ccgggcagta
    14281 taagcgtact ctgacgttgc tgggtgaaaa taccgaagaa gggcgggaga aattccgcga
    14341 agagttgaac gaaacgcatc agttgtttaa agattttgtg aagcgtatgc gtccgtctct
    14401 ggatattgaa caggtggcaa cgggtgaaca ctggtacgga caacaggcgg tagagaaagg
    14461 cctggttgat gaaatcaaca ccagtgatga agttattctt agcctgatgg aaggccgtga
    14521 agtggttaat gtacgctata tgcagcgtaa acgactcatt gaccgattca ccggcagcgc
    14581 ggcagagagc gccgatcgat tgttgctacg ctggtggcag cggggtcaaa agccattgat
    14641 gtaaaagaca aacgcgaggc taagacctcg cgttttgctt taatcaacca gatgatattt
    14701 ttctgaaagc acatgggcca ggtgtttgaa catattaaac accgcggtgc ttttggctgt
    14761 tggcaatcct tgttcatcta aaaagtagtc gccggtaaat accagcacgc cattacgctg
    14821 cgtgacgccg gtggcttcaa tccctgcgag cgtatcctca tgctcacgaa tgattttgtt
    14881 ggcctctttc aacagcgttt cgcggtcgat gggttgtgtc tctttgttca ttatttactc
    14941 cttaaacaag gacattagtc tacgccaggc atggcttgca gacaaatata ccacgctggt
    15001 ggcaagagcg ccttactggt aactttggat tttgcatgct aataaagttg cgtatcggat
    15061 tttatcaggt acagtgtgac gctttcgtca atctggcaat agatttgctt gacattcgac
    15121 caaaattccg tcgtgctata gcgcctgtag gccaagacct gttaactcag tcacctgaat
    15181 tttcgtgaac agagtcacga caaggggttg atatccgcag agaacgagtc catatcggta
    15241 actcgttgcc agtggaaggt ttatcaacgt gcgacgcatt cctggaagaa tcaaattagg
    15301 taaggtgaat atgggtaaag ctcttgtcat cgttgagtcc ccggcaaaag ccaaaacgat
    15361 caacaagtat ctgggtagtg actacgtggt gaaatccagc gtcggtcaca tccgcgattt
    15421 gccgaccagt ggctcagctg ccaaaaagag tgccgactct acctccacca agacggctaa
    15481 aaagcctaaa aaggatgaac gtggcgctct cgtcaaccgt atgggggttg acccgtggca
    15541 caattgggag gcgcactatg aagtgttgcc tggtaaagag aaggtcgtct ctgaactgaa
    15601 acaactggct gaaaaagccg accacatcta tctcgcaacc gaccttgacc gcgaagggga
    15661 agccattgca tggcacctgc gggaagtgat tggtggtgat gatgcgcgct atagccgagt
    15721 ggtgtttaac gaaattacta aaaacgcgat ccgccaggca tttaacaaac cgggtgagct
    15781 gaatattgat cgtgttaatg cccagcaggc gcgtcgcttt atggaccgcg tggttgggta
    15841 tatggtttcg ccgctgctat ggaaaaagat cgctcgtggt ctgtctgccg gtcgtgtgca
    15901 gtcggtggcg gttcgcctgg tggtcgagcg tgagcgtgaa attaaagcgt tcgtgccgga
    15961 agagttctgg gaagtcgatg ccagcacgac cacgccatct ggtggagcgt tggcgttgca
    16021 ggtgactcat cagaacgaca aaccgttccg tccggtcaac aaagaacaaa ctcaggctgc
    16081 ggtaagtctg ctggaaaaag cgcgctacag cgtgctggaa cgtgaagaca aaccgacaac
    16141 cagtaaacct ggcgctcctt ttattacctc tacgctgcaa caagctgcca gcacccgtct
    16201 tggatttggc gtgaaaaaaa ccatgatgat ggcgcagcgt ttgtatgaag caggctatat
    16261 cacttacatg cgtaccgact ccactaacct gagtcaggac gcggtaaata tggttcgcgg
    16321 ttatatcagc gataattttg gtaagaaata tctgccggaa agtccgaatc agtacgccag
    16381 caaagaaaac tcacaggaag cgcatgaagc gattcgtcct tctgacgtca atgtgatggc
    16441 ggaatcgctg aaggatatgg aagcagatgc gcagaaactg taccagttaa tctggcgtca
    16501 gttcgtagcc tgccagatga ccccagcgaa atatgactcc acgacgctga ccgttggtgc
    16561 gggcgatttc cgcctgaaag cacgcggtcg tattttgcgt tttgatggct ggacaaaagt
    16621 gatgcctgcg ttgcgtaaag gcgatgaaga tcgcatctta ccagcagtta ataaaggcga
    16681 tgctctgacg ctcgttgaac ttacaccagc ccagcacttt accaagccgc cagcccgttt
    16741 cagtgaagca tcgctggtta aagagctgga aaaacgtggt atcggtcgtc cgtctaccta
    16801 tgcgtcgatc atttcgacca ttcaggatcg tggctacgtg cgagtagaaa atcgtcgttt
    16861 ctatgcggaa aaaatgggcg aaatcgtcac cgatcgcctt gaagaaaatt tccgcgagtt
    16921 aatgaactac gactttaccg cgcagatgga aaacagcctc gaccaggtgg caaatcacga
    16981 agcagagtgg aaagctgtac tggatcactt cttctcggat ttcacccagc agttagataa
    17041 agctgaaaaa gatccggaag agggtggtat gcgcccgaac cagatggttc tgaccagcat
    17101 tgactgcccg acttgtggtc gcaaaatggg gattcgcaca gcgagcaccg gggtattcct
    17161 tggctgttct ggctatgcgc tgccgccgaa agagcgttgc aaaaccacca ttaacctggt
    17221 gccggaaaac gaagtgctga acgtgctgga aggcgaagat gctgaaacca acgcgctgcg
    17281 cgcaaaacgt cgttgcccga aatgcggcac ggcgatggac agctatctca tcgatccgaa
    17341 acgtaagttg catgtctgtg gtaataaccc aacctgcgac ggttacgaga tcgaagaggg
    17401 cgaattccgc attaaaggtt atgacggccc gatcgttgag tgtgaaaaat gtggctctga
    17461 aatgcacctg aaaatggggc gtttcggtaa atacatggcc tgcaccaacg aagagtgtaa
    17521 aaacacacgt aagattttac gtaacggcga agtggcacca ccgaaagaag atccggtgcc
    17581 attacctgag ctgccgtgcg aaaaatcaga tgcttatttc gtgctgcgtg acggtgctgc
    17641 cggtgtgttc ctggctgcca acactttccc gaaatcgcgt gaaacgcgtg cgccactggt
    17701 ggaagagctt tatcgcttcc gcgaccgtct gccggaaaaa ctgcgttatc tggccgatgc
    17761 gccacagcag gatccggaag gtaataagac catggttcgc tttagccgta aaaccaaaca
    17821 gcaatatgtc tcttcggaaa aagacggaaa ggcgactggc tggtcagcat tttatgttga
    17881 tggcaaatgg gttgaaggaa aaaaataacc tttaattctg tcaggttttt ataaacaaag
    17941 ggtcgcgaaa gcggcccttt tttattgcat attatttttt cttcacacct atacactaag
    18001 gctataaatg atatagtggt tatagttagc acctttttta ttattaaatc gtattagtca
    18061 cccgccaggt gtgacgaaaa aacgatgttc tgatggcgtc taagtggatg gtttaacatg
    18121 aaattacaac aacttcgcta tattgttgag gtggtcaatc ataacctgaa tgtctcatca
    18181 acagcggaag gactttacac atcacaaccc gggatcagta aacaagtcag aatgctggaa
    18241 gacgagctag gcattcaaat tttttcccga agcggcaagc acctgacgca ggtaacgcca
    18301 gcaggacaag aaataattcg tatcgctcgc gaagtcctgt cgaaagtcga tgccataaaa
    18361 tcggttgccg gtgagcacac ctggccggat aaaggctcgc tgtatatcgc caccacgcat
    18421 acccaggcac gctacgcgtt accaaacgtt atcaaaggct ttattgagcg ttatccccgc
    18481 gtttctttgc atatgcacca gggctcgccg acacaaattg ctgatgccgt ctctaaaggc
    18541 aatgctgatt tcgctatcgc cacagaagcg ctgcatctgt atgaagattt agtgatgtta
    18601 ccgtgctacc actggaatcg ggctattgta gtcactccgg atcacccgct ggcaggcaaa
    18661 aaagccatta ccattgaaga actggcgcaa tatccgttgg tgacatatac cttcggcttt
    18721 accggacgtt cagaactgga tactgccttt aaccgcgcag ggttaacgcc gcgtatcgtc
    18781 ttcacggcaa cggatgctga cgtcattaaa acttacgtcc ggttagggct gggggtaggg
    18841 gtcattgcca gcatggcggt ggatccggtc gccgatcccg accttgtgcg tgttgatgct
    18901 cacgatatct tcagccacag tacaaccaaa attggttttc gccgtagtac tttcttgcgc
    18961 agttatatgt atgatttcat tcagcgtttt gcaccgcatt taacgcgtga tgtcgttgat
    19021 gcggctgtcg cattgcgctc taatgaagaa attgaggtca tgtttaaaga tataaaactg
    19081 ccggaaaaat aatttcttgc gttattttcg gcacctttta tgtagcgaag gtgccggaat
    19141 atattctc
//
LOCUS       DAZFXT010000088        19092 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.088, whole genome shotgun sequence.
ACCESSION   DAZFXT010000088 DAZFXT010000000
VERSION     DAZFXT010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19092)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19092)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19092
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.088"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(45..623)
                     /gene="mocA"
                     /locus_tag="ACHKW0_003333"
     CDS             complement(45..623)
                     /gene="mocA"
                     /locus_tag="ACHKW0_003333"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HFO7476117.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWQQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     AHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            726..1496
                     /gene="yqeC"
                     /locus_tag="ACHKW0_003334"
     CDS             726..1496
                     /gene="yqeC"
                     /locus_tag="ACHKW0_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HFO7476118.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            1544..3169
                     /gene="yqeB"
                     /locus_tag="ACHKW0_003335"
     CDS             1544..3169
                     /gene="yqeB"
                     /locus_tag="ACHKW0_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HFO7476119.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFSSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(3210..4142)
                     /gene="arcC"
                     /locus_tag="ACHKW0_003336"
     CDS             complement(3210..4142)
                     /gene="arcC"
                     /locus_tag="ACHKW0_003336"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HFO7476120.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(4190..5575)
                     /gene="hyuA"
                     /locus_tag="ACHKW0_003337"
     CDS             complement(4190..5575)
                     /gene="hyuA"
                     /locus_tag="ACHKW0_003337"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HFO7476121.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(5628..6839)
                     /gene="ygeY"
                     /locus_tag="ACHKW0_003338"
     CDS             complement(5628..6839)
                     /gene="ygeY"
                     /locus_tag="ACHKW0_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HFO7476122.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQVYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(6897..8093)
                     /gene="dpaL"
                     /locus_tag="ACHKW0_003339"
     CDS             complement(6897..8093)
                     /gene="dpaL"
                     /locus_tag="ACHKW0_003339"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008838 - diaminopropionate ammonia-lyase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HFO7476123.1"
                     /translation="MSVFSLKIDIADNKFFNGETSSLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(8151..9338)
                     /gene="ygeW"
                     /locus_tag="ACHKW0_003340"
     CDS             complement(8151..9338)
                     /gene="ygeW"
                     /locus_tag="ACHKW0_003340"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HFO7476124.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9817..11589
                     /gene="ygeV"
                     /locus_tag="ACHKW0_003341"
     CDS             9817..11589
                     /gene="ygeV"
                     /locus_tag="ACHKW0_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HFO7476125.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQVEIVDENLCRVAGTG
                     AYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTPVI
                     LQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISKIF
                     ATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTHGH
                     MQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGECRV
                     MRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPEQL
                     LESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAREI
                     LPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQEDIE
                     LLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGEVI
                     DSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSKKQ
                     VADELGIGIATLYRKIKKYELLNT"
     gene            complement(11629..12108)
                     /gene="xdhC"
                     /locus_tag="ACHKW0_003342"
     CDS             complement(11629..12108)
                     /gene="xdhC"
                     /locus_tag="ACHKW0_003342"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HFO7476126.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(12105..12983)
                     /gene="xdhB"
                     /locus_tag="ACHKW0_003343"
     CDS             complement(12105..12983)
                     /gene="xdhB"
                     /locus_tag="ACHKW0_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HFO7476127.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(12994..15291)
                     /gene="xdhA"
                     /locus_tag="ACHKW0_003344"
     CDS             complement(12994..15291)
                     /gene="xdhA"
                     /locus_tag="ACHKW0_003344"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HFO7476128.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            15706..16461
                     /gene="actS"
                     /locus_tag="ACHKW0_003345"
     CDS             15706..16461
                     /gene="actS"
                     /locus_tag="ACHKW0_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase activator ActS"
                     /protein_id="HFO7476129.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            16540..16613
                     /locus_tag="ACHKW0_003346"
     tRNA            16540..16613
                     /locus_tag="ACHKW0_003346"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16572..16574,aa:Gly,seq:ccc)
     gene            16847..18457
                     /locus_tag="ACHKW0_003347"
     CDS             16847..18457
                     /locus_tag="ACHKW0_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HFO7476130.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            18470..18589
                     /locus_tag="ACHKW0_003348"
                     /pseudo
     CDS             18470..18589
                     /locus_tag="ACHKW0_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344669.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            18549..19092
                     /locus_tag="ACHKW0_003349"
                     /pseudo
     CDS             18549..19092
                     /locus_tag="ACHKW0_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YscQ/HrcQ family type III secretion apparatus
                     protein"
BASE COUNT         4826 a         4465 c         4840 g         4961 t
ORIGIN      
        1 ctggagagat taaatatttg aacgcaatcg tttaactaca ttttctaaat tttagtatac
       61 cttttttgcg ctgtaataaa gtcgtccgga gtatcaatat ctaaaattat ttctgcattt
      121 tctatctcga cggaataatg agctcccata aggagtgcct gacgcatatt ggtgacattg
      181 ggtcgttgga ttgcctgcat caagcatggt tttgacacta aaatcggatg gccggggatg
      241 ccattatgga gtggcagtat tgcgccatca tttcgtaacg accagatttt tctaaaaata
      301 tcgatggtga gggttggcat gtcaccgtgg gtgagaaaac aatgttctgt ttgcactgcg
      361 ggtactgcgg cctttactga tgttagtaaa ccctgcgcat aatctgggtt gtggataata
      421 gtgatattgc tctggttcgc ataacgttcg tgcagttcat taccacgata gccggtgact
      481 aaaataattc ggctacaaaa ctgcaacgca tttttgatac ttgtatcaag aattgtcccc
      541 tgttgccagg gtaacatcat tttccattgc cccattcttg atgataatcc ggcagcggta
      601 attatacagt cgatggctga catagtggtt ccattattta taggggctaa taacgcctga
      661 taattgcgtt acttcctgat ggattaagtt ggggataatt tatcccagag aggtcataaa
      721 gactcgtgaa aagtatagtt gacccatcgg cattagtcat tgatttaggt gcgcagaaac
      781 gtcccactgt gatttctgtt gttggtgctg gggggaaaac cagcttgctt ttttggctgg
      841 cagaactgct ccaggctagc ggcaggcgtg tgttaatcac gacgacgaca catatgttta
      901 tgccaacgtc tcactggccc gtggttttct gtcgtgatcc cgccatgctt cctcatgcgt
      961 ctcttacatc ccccatttca ttttgttttc acagctggaa agcgaaccag ggaaaagtgc
     1021 agggatttac gccagaagcg attgatgcac tggtgcaacg accagagtgt gacgtaattc
     1081 tcattgaagc agatggctcg cgtggaatgc cgttaaaagc gcctgatgag cacgaacctt
     1141 gcatacccaa aagcagttgc tgcgtgattg ctgtgatggg aggacatact ttgggcgcga
     1201 aagtgagcac agaaaatgtc catcgctggt cgcagtttgc tgatattacc gggttaacac
     1261 ctgatgcaac cttgcaactg agcgatctcg ttgcgctggt tcgccaccct cagggggcgt
     1321 ttaaaaacgt accgcaaggt tgtcggcgag tctggttcat taaccgtttt tctcaatgtg
     1381 agaatgcgat tgcacaaagc gagctccttc aaccgctgca acaacacgac gtagaggcaa
     1441 tctggctggg cgatatacaa gaacatcctg caatcgcgcg cagatttgtg aattagcggc
     1501 ccgggtattc cgggttactg aaaggatatc cattgaggtt ggtatgaata ttttcacaga
     1561 ggctgcaaaa ctcgaagagc aaaattgtcc gtttgcgatg gcgcaaattg ttgatagccg
     1621 aggctcgact ccccgccatt ctgcacaaat gttagtgcgc gccgatggtt ctatcgtcgg
     1681 tacaattggt ggcggaatgg ttgagcggaa ggtgattgaa gagtcacttc aggcattgca
     1741 ggaacgtaag ccgcgattat tccatggacg tatggctcgt aacggtgcgg atgctgtcgg
     1801 gtcagattgt ggaggtgcaa tgtcagtatt tatcagcgtc catggtatgc gtccacgtct
     1861 ggtgttgatt ggcgcggggc atgtcaaccg ggcgatagcc cagagtgcgg cgctattagg
     1921 atttgatatt gccgttgccg atatttatcg cgaaagcctc aatcctgaac tattccctcc
     1981 atcaaccacg cttctccatg ctgagtcgtt tggtgcagca gtggaagcac tggatattcg
     2041 ccctgataat tttgtcctga ttgccacgaa taatcaggat cgtgaagccc tcgacaaact
     2101 cattgaacag cccattgcat ggttggggtt gctggcaagt cgccgcaagg ttcagctttt
     2161 cctgcgtcaa ttgcgtgaga aaggcgtggc tgaagaacat attgcccgtt tacatgcgcc
     2221 cgttggttac aacataggtg cggaaacgcc gcaggagatc gccatcagcg tgttggcaga
     2281 aatattacag gtgaaaaata acgcgccggg tgggctgatg atgaaacctt ctcatccttc
     2341 cggacaccag ctggtggtga ttcgcggtgc gggagatatc gccagtggtg tggcgctacg
     2401 tctgtatcat gcgggtttta aagtgatcat gctggaagtg gaaaaaccga cagtgattcg
     2461 ttgtacggtg gcgtttgccc aggccgtgtt cgatggcgaa atgacggtcg aaggcgtcac
     2521 tgctcgcctg gcaaccagct ctgcggaagc gatgaaactt accgaacgcg gattcatccc
     2581 tgtgatggta gatcccgcct gttcattgct tgatgaactg aaaccgcttt gcgtggtgga
     2641 cgctattctg gcgaaacaga atttgggaac gcgggcagat atggcaccag taacaatcgc
     2701 gcttgggccg ggctttactg cagggaagga ttgtcatgcg gtaattgaaa caaatcgcgg
     2761 gcactggctc ggtcaggtga tttactctgg ttgtgcgcag gagaataccg gtgttcctgg
     2821 caatattatg gggcatacca cccgacgggt gatccgtgct cctgctgcag gcattatgcg
     2881 atccaacgtg aaattaggcg atctggtgaa agagggcgat gtgattgcct ggattggtga
     2941 gcatgaaatt aaagcaccgt tgacggggat ggtgcgtggc ttgttgaacg acggcttggc
     3001 agtggttggt ggttttaaaa ttggtgatat cgatcctcgt ggtgaaaccg ctgatttctc
     3061 cagcgtttct gataaagccc gggcgattgg cggcggcgta cttgaggcgt taatgatgtt
     3121 gatgcatcag ggcgtgaaag cgacaaaaga agtgctggaa gtggcttaaa tgaaaatggc
     3181 cggatggtat tacccacccg gccttaaaat tactgacaaa tcaccgttcc ggttttgcct
     3241 tcaatccctt ctttcgcttt gctcaatacg gtaatcagtg cttcacgacc tgcgcgagag
     3301 cgagcaaacg aggcggcagc ttccacttta ggcagcatag aacctttcgc aaaatgacct
     3361 tcttcgataa agcgttcagc atcacttaat gacaggcgat cgagccactg ttcattctct
     3421 ttaccgaagt taatggccac tttttctacc gcagtcagaa tgatcagcat atcggcatca
     3481 atcatttctg ctaaacgggc gctggcccag tctttatcga taaccgcgct ggcaccacgc
     3541 agatggttac cttcacgaat aacaggaata ccaccaccgc caacggtgat caccacctgg
     3601 cctgcatcta ccagagcttt aaccgtttct ttttcaataa tatcaaccgg ttttggcgag
     3661 gcaacaacac ggcgatagcc gcgaccagca tcttctttca gagtgtaacc ctgttttgtc
     3721 agttgctctg cttcttgctc ggtaaagaac gaaccgatcg gcttggtcgg gttgaggaat
     3781 gccggatcgt tagcgtcgac ttcgacctga gtaaccagcg ttgctaccgg tttattaatg
     3841 ccccgagaaa gcagttcttc tcttaaggcg ttttgtagat cgtaaccaat ataaccctgg
     3901 cttaaggcaa cgcagacaga catcggcagc attggcgagt gcgcttcagt ttttgcagca
     3961 gcttcaaagg cctggttaat catgcccacc tgcggcccgt taccatgagt gacgataact
     4021 tcatgtcctt gggcaattaa atcaacaatt gcctgagaag taatttttac cgctttcatt
     4081 tgtccggcca ggtcatcgcc cagcgcattc ccgcccaggg cgagaacaat tttcttactc
     4141 atttttcctc actcatcatt cttgattgtg tatagatccc tgacagtggt tagagcacgg
     4201 gagggacaaa cggtttgcgt cgcaggaaac gacctcgccc ggctttgcct gtaaaggtgc
     4261 cgtcacagaa aatcgtttca ccacgggata aggttctgac aatcgcgccc tgacaggtaa
     4321 aaccctccca tggcgagtag tcggcgttgt cgtggagatg gcgatgctga atttgttggc
     4381 tctgacgtgg gtcgataatc accacgtcgc catcggaacc gggcgctaat aatccttttt
     4441 gcggccacag gccaaacagc ctggcgggca ttgcgctggt taattcaaca aagcgttccg
     4501 gtgttatacg tcccgtcatc acgccactgg aaaacagtaa ctgcatgcgg ttttccacac
     4561 cgggtaagcc atttgggcag cgactgaaat cgcctttaga aatttgcagg cgttgagcca
     4621 tcgagaaggt gcagtgatcg gtcgccacca cgtcaatcgc accatcgctg atgccacacc
     4681 acagtttgtc ctgctcgcgt acgttacgca gcggtgggct aagaatgaac ttcatgccgt
     4741 cttctgtatc gtaactgcgt tcgtccaaca ggagatattg tgggcaggtt tcaacccaga
     4801 ctggctggtg attcgcacgg gcaagacgca gataatccag acctaagccg ttagacaggt
     4861 gcacgatata gagcggggcg ttaccggcaa tttgtgccag gttaatcatg cgggcgatgg
     4921 cttccgcttc gcattccaga gggcgactca aggcatgata gcgcggcgcg gttaaccctg
     4981 cggcgataaa ttccgcccgc ttgctggcga tagccgcatc attttccggg tgcacggtgg
     5041 tcagcgcgcc ggattcatgc agacggcgta atgcctgcaa aacctcgtca tcgttgagtt
     5101 tgtattgata ggttaaatag agtttaaaac tgctcagtcc ttcctcgacc atcatcggga
     5161 tttcgtcgag gattgcgtga ttaatgtgct ggatcacacc gtgaaagctg taatcgatga
     5221 ccgctttatg ggcggcataa ccacgataaa cctccagttg atggcgtaac cgacagccgt
     5281 ttgggccaaa tcccatatgg tcaataatgg ttgttgtacc gccacacgca gctgcgcggg
     5341 taccggtaaa aaaatcatca caactgcgcg cgatgccgac atcaatattg aaatgcgtat
     5401 ggacatccac gccgccaggg aaaacgtaac agccagtggc atcaatttct tcatacggga
     5461 gctgcggcga aatattgttg cccaactggc gaacaatccc gctttcaatc agcaaatcct
     5521 gtttggcttg tccatctgcg ttaacgacag tgccgttttt gatcaatacg cgcatagcaa
     5581 actccaaaaa ccctccggcg aaccggaggg gaataagtga agagtaatta ctctgttgcc
     5641 agccagctta acgggattgc agcgtacatc gcggcacagg tcaccaggtg agatttccag
     5701 gttttttcgt taggtgcatg cgcttcaggt tctttacccg ggccaaagcc gatcaccgga
     5761 atgccgtgac ggcccatgat agatacgccg ttagttgaga aggtccactt atcaacaacc
     5821 ggcgctttgc caaacagccc ttcgtaggca ttcaccagtg ctttaacggt gaagtgatct
     5881 tcttccactt tccaggtcgg gaagtagcat tcggttgggt aaaccaggcc agtccaggac
     5941 ggacggtcgt agttgtacat agaaacaacc gcgttagctt tctgtactgc aggcagggcg
     6001 cggatttcgt ccagcgcgcc ttcccaggtt tcgccccagg tcagacggcg gtcaatagag
     6061 acggcgcagc tatctgctac agcgcaacgg cttggggatg tgaagaagat ttcagaaacg
     6121 gtgagggtgc ctttgccgag gaattcgtca taacccagac gttgggagag ttcttgtaat
     6181 tcgccaagaa tcggacccat tttgaaaatg gcgttgtcac cgcgttctgg cgcagaaccg
     6241 tggcagctaa caccctgaac atcaatacga atttccatac gaccgcgctg accacggtat
     6301 acctggcagt cggttggttc ggtactgacc acaaattccg ggcgaatgcc ggattgttca
     6361 ataatgtact gccagcacag accgtcgcag tcttcttcct gcacagtacc ggtaaccagc
     6421 agggtatatt catcttccag accgaggtct ttaatgattt taccggcata aaccatagat
     6481 gccatgccgc cttcctggtc ggaagtaccg cgaccaccga tcagctcatc agtttccatg
     6541 ccttcgtacg gatcgaagtc ccagtttttg atgttgccaa tgccgacggt atcgatatga
     6601 gcgtccattg ccaccagacg cgggccgtgg ccgatataac cgagaacgtt gcccatcggg
     6661 tcgatttcaa ctttatcgaa gccgactttt tccatctctt ctttaatacg atgtactacg
     6721 cgtttttcgt cgcagctttc actgggaata gcaaccatgt cgcgcaggaa gcgagtcatg
     6781 tccgcctggt aatcttttgc tttttcaaga atcagtttga atggaatatt cttagccatt
     6841 atttttctcc agatttttta ctccccgttt tgcagggagt agttccaata gggtgattaa
     6901 ggtgctacag cgtgtttgcc ttcccagaca acttcgcggt agtgcttcac gtcggtgtcg
     6961 ccttcagtgc tgataaccag caccacggca tctttgttca gcgccagttt ttccatcagg
     7021 ctttgacgtt gcgggtgata atgaaccgct gcgagaacgc ccaaaccgac agcgccggat
     7081 tcaccggaga tgatgcgcgg gtcgttgccg tacggattac ccagcacgcg catacctaat
     7141 gcggcaacgc tgtcctggca ggagatgaat tgggtggcgc agttacgtag gatttcccag
     7201 cccagcgggt taggttcgcc acaggccagg cccgccatga tggtggccat atcaccgcca
     7261 acgttgacga tgtcgccttt gacgccggag cgataaatac agtcagcttt gtcaggttca
     7321 acaataatgc tgtgcagatt ttgcgggctg tagacgtcga ccagataacc cagcacacca
     7381 ccggccattg ctccgacacc ggcttgcagc agaacgtgcg tcggggttac gcccatttca
     7441 cgcatttgct cgacggcttc atctgccagg gttgcgtagc cttgcatgat ccaggttggg
     7501 attttggtgt aaccttccca tgccgtgtcc tgtaccactt cccagccgtg ctgctgcgcg
     7561 tgttgcatgg tcaggcgaac ggtatcgtca tagttcatat ccgtgacgat gcactcggca
     7621 ccgaggttca gaatggcgtc aacgcgttcc tgagcagaac ctttcggcat gtaaatcacc
     7681 gcattctgtc cgagttgctg tgctgcccac gccacaccgc gcccgtggtt gccgtcggtg
     7741 gtggtcgcga aagtcatttt ttcgccgatg gcatttttca ggtgctcaaa tgacagcgtt
     7801 tcgatatcaa gatgatattt ttcacacaat aattgagcga tggcgtacgc accgccaagc
     7861 attttgaagg cgttcagacc gaatcgtttt gattcgtctt tgacgagaat tttcttcaca
     7921 ccaaaaaggt ttgcgagatc gtccagcgca caaagcggtg ttgggcgata accagctatt
     7981 ttctggtgga actggcgcgc cagtttggcc tggctttgcg aaaagagcga tgatgtttcg
     8041 ccgttgaaaa atttgttatc ggcgatatca atcttcaatg agaaaacgga catatcctat
     8101 ccttttggcc tctggaaata gagggccgac cagctgatcg gcccgagggg ttatttcacg
     8161 cgttcttgcg cttctttcag cagttgctcg agcagtgcac ctggtttggc gtatttacgg
     8221 gacaggatca tcgcggcgat gatataaggc ttccagctgg cttctttgta ggtagcgata
     8281 cggtattttt cgaatacgcc ttcagtcact tccccttctt tgcaggatac gccgctgata
     8341 tcagctggca ggcagtgcat gtacagggct tcgccatcac gggtcagttc catcatctct
     8401 tcagtacaat gccagtcttt gtgttgcgcg ttctgtgcca gacactgttt ttccagtgct
     8461 tttaagcctt cgtgatcgtt agcgcgcagc aattcggtac gctgttccat cactttgtaa
     8521 ggtgcccatg acttcggata aacgatgtct gcatctttga atgcttcttc catgctggtg
     8581 acctgacgga agctaccgcc agatgcttta gcattgtttt tcgcgacttc tactacatcc
     8641 gggatcaggt cgtagccttc cggatgggcc agggtgacat ccataccgaa gcgagtcatc
     8701 agaccgatga tgccttgtgg tacagagagc ggtttgccat agcttggaga gtaggcccag
     8761 gtcatggcga tttttttacc tttcaggttt tccagtgaac caaagtgttc acgtaaccac
     8821 gcgaggtcag ccattgactg agtcgggtgg tcaatatcgc attgcaggtt cactaaagcc
     8881 ggacgctgtg gcagtacacc ctgcttgtaa ccgtcatcaa gtgcagcgcc aacttcacgc
     8941 atataggcgt tgcctgcgcc cagatacata tcgtcgcgaa taccaatagc gtcggcgcag
     9001 aaggagatca tattggcggt ttcacgcacg gtttcgccgt gagcgatttg tgatttgcct
     9061 tcgtcgagat cttgttgtgc aaggccgagc aggttaagcg cggaagcata agagaagcgg
     9121 gtacgagtgg agttgtcgcg gaatacggaa atacctaatc cactattaaa gactttggtt
     9181 gagatgtttt cagcacgcag tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt
     9241 tcatctggcg tctgttccca cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc
     9301 gaattgatat ccttaatcag ctcattaaca gttttcatca taaagtcccg gtagtggaat
     9361 gacaggcaaa gtggtttatt tcactgtgac ttgccaaagt gattggtgtt ttcacagcta
     9421 tacctgggac taacaataag tgtgccagaa ttatccacgg ggctgtaaaa gactaatttg
     9481 acggataatg tgtggggttg atcacaaatt gatgccagct tcgggttgtt tgtaaaatac
     9541 gaaaacgatt ggcttttctt atcgcgttat ttatttactg ctaagtatca gaaataagat
     9601 aaagggacag gctatctctc ttcagtttga taaactcacc gccgtaattc tcatattgat
     9661 atagaaatgt gatatctctt ccataatttc attagaaagt gataaaaatc ttatgttttt
     9721 atcaaagcca atttcgcctg agttaatatc atcatgatct gtgaaccatc aacgtcttca
     9781 atgatcgact tttaagtaag tctggaaggt aaagctatgg agcttgctac tacgcagtca
     9841 gtattgatgc aaattcaacc gacaattcag cgttttgcca gaatgcttgc cagcgttttg
     9901 caggttgaga tcgttgatga aaacttgtgt cgcgtcgccg gaactggcgc gtatgggaag
     9961 tttcttggcc gccagttgag cggcaactca cgcctgctcc gccacgtcct ggaaacgaaa
    10021 actgaaaaag ttgtgacaca gtctcgcttc gatccccttt gcgaaggttg cgatagtaaa
    10081 gaaaattgcc gcgaaaaagc atttctgggt acgcctgtca ttttacagga tcgttgtgtt
    10141 ggggtgataa gtttgattgc cgttacccac gagcaacaag agcatatcag tgataattta
    10201 cgcgaatttt ctgattatgt tcgccatata tccaccattt ttgtttcgaa acttctggag
    10261 gatcaggggc caggagataa catcagtaaa atattcgcga ccatgatcga taatatggat
    10321 cagggcgtat tagttgttga tgatgaaagt cgggttcagt ttgttaatca gactgcctta
    10381 aaaacacttg gtgttgtaca aaataatatt attgggaaac ctatccgttt cagaccatta
    10441 acatttgaga gtaattttac tcatggacat atgcagcata ttgtttcgtg ggacgataaa
    10501 agtgaattaa tcattggtca attgcataac attcagggcc gacaattatt tttaatggca
    10561 tttcaccaat cgcataccag tttttctgta gcaaatgcac ctgatgaacc acatattgaa
    10621 caattggttg gcgagtgccg tgttatgcgg caattaaaac gactcattag ccgtattgca
    10681 cccagcccat ccagcgttat ggtggttggt gaaagcggca cgggtaaaga agtcgtcgcc
    10741 cgagcaatcc ataagttgag cggaagacgg aataaaccct ttattgctat caactgtgcc
    10801 gcgattccgg agcagcttct ggaaagcgaa ctgttcggtt atgttaaagg cgcatttact
    10861 ggcgcttctg ccaacggtaa aacagggttg attcaggcgg cgaatacggg cacgctgttt
    10921 ctcgatgaaa taggtgatat gccattaatg ttgcaggcta aattactgcg cgctattgag
    10981 gcgcgtgaaa ttctgccgat tggtgccagt agcccaatac aagtcgacat tcgcatcatt
    11041 tctgcaacta atcagaattt ggcccagttc attgccgaag gtaaattccg cgaagatctc
    11101 ttctaccgac ttaatgttat cccgataact ctgccaccgc tgcgtgaacg tcaggaagat
    11161 attgaactat tggtgcatta ctttttacat ctgcataccc gtcgtctggg atcggtttat
    11221 cctggcattg ctcccgatgt cgtcgaaata ttgcgtaagc atcgttggcc cggaaacctg
    11281 cgcgagttaa gcaatttgat ggaatatctg gttaacgtag ttccttcagg tgaagttatc
    11341 gacagcacgc tattgccgcc aaatctgctg aataatggca caacggagca aagtgatgta
    11401 acagaggtca gtgaggcgca cctgtcactc gatgatgcgg gcggcacggc gctggaggag
    11461 atggaaaagc aaatgatccg cgaggcgctt tcacgtcata acagcaagaa gcaagttgct
    11521 gatgaactgg gcatcggcat tgctacgctc tatcgcaaga ttaagaaata tgagttgtta
    11581 aacacataac ctcagtgaat ccggcctgaa ttcaggccgg atatcctttt acttcgtttt
    11641 ctcgcaatcc agaactgtat ttacaatcat ctgatacccc gtgcagcgac aaagatttcc
    11701 cgccagtccg cgacgaattt ccgtaatggt taatggcttc tcgcgtggtt tcgccagcat
    11761 tgccgtggta gccataatca ggccaggcgt acaaaacccg cactgcactg cgccggattt
    11821 cgcataagcc tgctgaacat gagaaagttt tccgcctttc gcttcacctt ccagcgtgcg
    11881 gatctctttt ccttcagccc aggcggcaag gtataagcaa ctgtctattg ctgtgccgtc
    11941 gaccagcacc gtacaggcac cacattcacc cacgcagcac ccttgtttga cacttagcag
    12001 tccttgttcg cggagtaatt ccgagagcgg cgtgcctggt gcggcgtgaa gctgaaaagg
    12061 catcccgtta atggtgcatt cgatggtaat tgtttcgctg tgattcattg caattttccc
    12121 cccgccgcgg cgacggcttc gctaatcact tttttggtca tcgtctggat gagatgcaga
    12181 cgaaactctt tactggcccg ccatgaagaa cgcggggcga catcttgcag gacagattcg
    12241 ctgatagctt ccagcgtttg caggtttaat ggcgcatttt gtgcagtctg ttcggcatgt
    12301 tggcagcgaa tcggcgttgg cgcggcaaca ccaaatgcca ggcgtaattc gctgaaattg
    12361 ccgttatcca gtcggcaatg tgcggcgcag ccaatcgttg aaatatccat tgcgtcgcgc
    12421 atggcatatt taaaatgcgc gctgcccgcg tgttctttcg gctgtggcgg aaaatgaaag
    12481 gcgacgagga tttcgtcatg ctcaagagac actttgcccg gcccggtgtg aaagccatta
    12541 atcgggacga aacgaacacc gcgcggggag tggatctcca gtttcgcgtc ataaattagc
    12601 gttggcgtgg cagaatctgc gctggtggca ccgttgcaaa tatttccacc gtaggtagcg
    12661 acgttacgga tctgcggtcc agcaatggac gtggccgcag cacataacgc cgggagatga
    12721 cgttgagtta taggatcttc tattagctgg gtaaatgtcg ttgcagagcc gatacgtagc
    12781 gagccatctt ccgccagcgt aattccccgc agctccgcca gattatgaat atcaacaata
    12841 tggcgataac ggtcattgtg atggtggagc tgaatcagta cgtcagtgcc accggcgagc
    12901 agtttggcct gcgggttgtc agccagcagg ttgatggcat cggcaagggt tgctgcgcga
    12961 tggtaagaag caaaatcaaa catgatgtta tcctcaaatc aatcctgcca gatggaactc
    13021 ttcatataac cgttttggcg tcagcggcag tgtattgatt gcaacaccgg tagccatctt
    13081 caccgcgtta cgaatagcag cggcaacagg aattattggt ggctcaccca gtgacttatg
    13141 tccgtatgcg gattgcggct cattgatttc gacgaacgcg ctttccagtt gtggcagatc
    13201 cggcatggtc ggcattttgt aatccagcag attggggtta cggaccacgc cgcttttagc
    13261 atcgatgatc atctcttcaa atagcgccca gccaatgccc attcccattc cgccgtgtac
    13321 ctgaccttct gccagcagtg gattaagaat atgccctgaa tcatgaacgt tgaggatgcg
    13381 gttgatggtg actttgcaca gcgcaatatc gaccgtcaga tcaacaaagg tacagccaaa
    13441 cgccggtggg ttagtggtgg ttttgatgga gctttcagca gagagctgcc cgccgcgttc
    13501 agggtggtag aaagcgtcca tcgccaaatc ttttaacgac attaacggct cttccggtcg
    13561 ttcaaccagc acgatatggc cttttatcag ggtcagattc atcgctgact gatgtagcat
    13621 gactgcggcg tgagcgatga ttttctcttt taataatagt gccgcactgc gcagcgcagg
    13681 cgcggcaaca tagctctggc gtgaggcaaa tgcgccggga tcgaacggcg taacgtcggt
    13741 atcttgagtt gaaataacgc gaacgtcgct gaccggaacc cccacggttt ctgccaccat
    13801 ttgcgagaag acggtgtcgg caccctgacc gatttccgtc gcgccgcttt gcacgttgat
    13861 ggttccatcc tgattcatca gaaggcgcgc gcctgctatt tctacgccga caggccaggt
    13921 gttagaggtg tagctaaaac aggcgacgcc aacgccgcgg cgcaaattgc cttgctggtt
    13981 ctggcattct gcacggcgtt tttcccattc aaagattttc cggccttttt caagacactc
    14041 cggcaaccct gcgctgtaaa tacgtttgcc cgtgagcgga ttagcatctc cttcgcgggc
    14101 ggcgttgcgt aaacgaattt caacaggatc aatacctaac gctgtcgcgg cgtcatcaag
    14161 catagactca acggcaaata cgacttgtgg cgcgccataa ccacgcatcg caccagccga
    14221 ggggaggttg gtatagcagg tctttgaact gtaagcgtag gcacaacgag gataaaggta
    14281 agcgacttta ttcccccccg cagaagcgat ggagtgcccg tgagatgcat aagcgccggt
    14341 gttagacaga acatccagac tataaccttt caatgttccg tcgcggttca cgcccatttg
    14401 cccgtcaatg gtaaaagcgt ggcgggtacg ggttgcgagg aaacactctt cacggctaag
    14461 ggaaactttc accggaatgc cgccaagctt gctggtcagg aatgccgcca ttggctcttc
    14521 cagtacatcc tgtttattac caaaaccgcc accgacaaac ggtttgatga ctcgtacgca
    14581 tgaccaggga atatccagcg cctgaccaac cacgcggcga acaatgtgcg ggatctgggt
    14641 gctggaaacg atggtaattc gcgagtcatc ctccatccac gccagcgatg ttacgctttc
    14701 catgtgacaa tgttgaataa cgggggtctg atagtgcccc tgtacctggt agtcggcggc
    14761 atcgattgtt tgttggacat tacccgtcga catcgtgctt tgtttcagta aattgccacc
    14821 gttatggatt ggtgcagcgt cttctgccag cgccgcttct ggcgtggtga taacgggtaa
    14881 ttcttgccac tcaatgctga ccaattgcgc cgctttttct gccgtgagtt catcgcgggc
    14941 cacgacgatg gcaacggcgt caccatgatg acgaacatgg cgagttagca gtgcgcgatc
    15001 ggcggtatcg cgcttgtttt cgtcaagtgt ccaggcatgc ccagcagtag caaatggaat
    15061 atcaggcaca tcttcccagg taaaaatcgc cagtacgccc ggcaaacttc tggcttgttc
    15121 atcattaata cttacggcat aaccatgtgc gatagggcta cgtacatatt tcgcataaca
    15181 catgcccgcc ataacataat cgtcagtata tcgtgcccgc ccggtgacct tagcaatggc
    15241 atcgacgcgc atgcatgatt cacccgtagc ggttgcttcc cgcgcttcca tagaaatccc
    15301 ctcgatttga acatttggtt cttatggcaa agatttagtc taagcaggca agatttacgc
    15361 cagaaacgac gataagcgtt aggggaggta ggaagtgtga tgacgttaaa aaaacacaga
    15421 tatgatacga aacaggactc tgaatagggg cagtgatacg tatcaatatg agtggcgata
    15481 aaatcaatgt gataaattgg gattattacg catcaggatg ggaaataggg taaaaagtca
    15541 ggaagttcga tacctctaca gcgaatattt tgctgatgat acttcccttt gccaaatgaa
    15601 attaacctct ttctctatta ccacgttttt ccagaagcaa gagattgggt atcaagaggc
    15661 tggctgctat gataaggcgt cttgtttttt aagcgaggaa agattttgag tgcgggacgc
    15721 ctgaataaaa aatctctggg tatcgtgatg ttgttatcgg ttggactgct tttggcgggc
    15781 tgttcgggta gcaaatcatc cgatacagga acgtattccg gctccgttta caccgtgaaa
    15841 cggggggata cgctatatcg tatttcgcgc accacgggaa ccagcgtaaa agaactggcg
    15901 cgactgaacg gcatttcccc cccttacacc attgaagttg gtcagaaact aaaactgggt
    15961 ggggcgaaaa gtagcagtag tacacgtaaa tcaaccgcca aatcaacgac caaaaccgca
    16021 tcggttacac cgtcatcagc ggtaccgaaa tcttcctggc cgccagtagg gcaacgttgt
    16081 tggttatggc caacgacagg gaaagttatc atgccgtatt cgacagcaga tggcggcaat
    16141 aaagggattg atatctcagc tccacggggc acacctattt acgccgcggg tgcaggaaag
    16201 gtggtgtatg tgggcaacca gctgcgtggc tacggtaatc tcatcatgat taaacacagt
    16261 gaagattaca ttacggctta cgcccataat gacacgatgc tggtaaataa tgggcaaagc
    16321 gtgaaggctg ggcaaaaaat cgccactatg gggagcacgg atgcggcatc tgttcgcctg
    16381 catttccaga ttcgttaccg tgcaacggca attgatccgc tacgttactt gccgccgcag
    16441 ggcagcaagc caaaatgctg atggcgaatt aatcagcagt cagcagcgcg gcacttgcta
    16501 aggagagcgt aaggtttata atgccttacg catctcgaag cgggcgtagt tcaatggtag
    16561 aacgagagct tcccaagctc tatacgaggg ttcgattccc ttcgcccgct ccaatttatc
    16621 ttcacctgat caatctgcca tttttgctgt atctgatttt ctatattatc ttaattcaat
    16681 cgcttcagac tattcgcaat cgccgtaata cttccccatt ttgttgtaaa taaaaagcgt
    16741 ttacgcaatg caatcctttt gtaacaaccc tggtaaaaca ccttattttc aagttgttaa
    16801 ataagtggtt atctacgctt ttaacaaacg attgatggaa tattttatga aagggaaatc
    16861 tgcattaact cttctgctcg ctggtatatt ttcgtgcggc acatgccagg cgacaggggc
    16921 agaagtgacg agtgaatctg tatttaacat tcttaattcc acgggggcag caacagataa
    16981 aagttattta tcgttaaatc cagataagta tcctaattat cgtcttttga ttcactcagc
    17041 caaattacaa aatgaaataa agagccacta taccaaagac gaaattcaag gattgctaac
    17101 attaacagaa aacacacgaa agttaacgtt gacggagaaa ccctggggaa cgtttattct
    17161 tgcttctact ttcgaagatg acaaaacagc ggcagaaacc cactatgatg ctgtctggct
    17221 gagggatagt ttatggggct atatggcatt agtatcagat cagggaaata gtgtagccgc
    17281 aaaaaaggtt ctgctcaccc tgtgggatta tatgtctacg cccgaccaga ttaagcggat
    17341 gcaggacgtc attagcaacc ccaaacgact ggatggagtt cccgggcaaa tgaatgttgt
    17401 gcatatccga tttgacagca actcccccgt gatggcagat gtgcaggaag aaggcaagcc
    17461 gcagctgtgg aaccataaac aaaatgatgc gttaggtctt tatcttgatc tccttataca
    17521 agcaatcgac actgatacaa ttaacgctga agattggcaa aaaggtgacc gattaaaatc
    17581 ggttgcgctg ctgattgctt atctggataa agccaatttc tatgttatgg aagattccgg
    17641 agcatgggaa gaagatgccc gattaaacac atcttccgtt gcactggtga cctctggact
    17701 ggaacgtttg tcgaacttac tctcgaagaa agactctgtt tttgtctctg atttactacg
    17761 cgaagctaaa gccaatgaac tggatgaacc tctttcaaca acgaggttga accacctcat
    17821 agataaaggt tatgaaagaa taacgttaca gcttgattta gggggagaat ccccaggtta
    17881 tctggaaaaa gataaacatt acagagaagc cgatgcggct ttacttaatg ttatttatcc
    17941 tgcaaatctt gcaaagataa acaccagaag aaaagagcag gttctaaaaa tagttaaaaa
    18001 actagcaggt ccgtatggta taaaaagata cgagaaggat aattatcagt cggcaaattt
    18061 ttggttcaat gatattaaaa ccgataccga tcaaaattca catgcgaaaa gagaaaagag
    18121 cttcattcca tcaacagaag cagaatggtt ttttgactca tggtatgcaa aatcagcagc
    18181 aattgtttat aaagaatcaa gaaaggaaga gtatttgaat gactcagtgc aatttatgaa
    18241 tagatcattg gcccaaataa caggcgagaa tatgattggt gcaaatggca ggagtgtacc
    18301 tgaaatggca cttcccgaaa gctataacta tatccacaag tcgggaactt tgcatgaagc
    18361 gccaagccct atcattccgc taaattggtc gaaagcttca atgactctca tgttgaaaga
    18421 gatgtccaac cttatcaatg atgagggaat taaatagttt tcataatgag ttacagcaca
    18481 acgttgggta ttgactgagc aagatgaacg acaaggacaa cgacatcagc cttatgagga
    18541 gcaagaaaat gaaagcaaat tcgaaaacga tcagaaggat gaaagttaat gttcggtctg
    18601 aggaaagtaa atcgaaacac tcatacgttt gaaaccactt ttcaaaattg gaaagaaaat
    18661 ggcgaagatg ttgcattatt aatgcccgaa tttagtgcta aatggttacc gatagcagaa
    18721 gagagtggca gctggtctgg atgggtattg cttggagaaa tatttccctt aatttcatct
    18781 gagctcgctg ggatggcttt aatgccagaa acggaaaggt taatagggga atggctcagt
    18841 ttatccagtt ccccgttaaa tctaaagcat cctgaactaa aatataatcg tttgcacgta
    18901 ggtaaagtat ttgatggagt attgaaccct gctcaaccac taataagaat atgaacaggg
    18961 gaattgaatc tttggttaga taaagtcaca gtctgccaat acggaaacgc tccagtgtta
    19021 gacaaaaaat cattatattg gtccattcat tttgtaattg gatttagtaa aacatgttac
    19081 agaagtcttg tt
//
LOCUS       DAZFXT010000089        18723 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.089, whole genome shotgun sequence.
ACCESSION   DAZFXT010000089 DAZFXT010000000
VERSION     DAZFXT010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18723)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18723)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18723
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.089"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            249..941
                     /gene="yieP"
                     /locus_tag="ACHKW0_003350"
     CDS             249..941
                     /gene="yieP"
                     /locus_tag="ACHKW0_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HFO7476131.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
     gene            964..2391
                     /gene="mdtD"
                     /locus_tag="ACHKW0_003351"
     CDS             964..2391
                     /gene="mdtD"
                     /locus_tag="ACHKW0_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HFO7476132.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GERIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(2357..3349)
                     /gene="rbsR"
                     /locus_tag="ACHKW0_003352"
     CDS             complement(2357..3349)
                     /gene="rbsR"
                     /locus_tag="ACHKW0_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HFO7476133.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(3353..4282)
                     /gene="rbsK"
                     /locus_tag="ACHKW0_003353"
     CDS             complement(3353..4282)
                     /gene="rbsK"
                     /locus_tag="ACHKW0_003353"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004747 - ribokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006014 - D-ribose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HFO7476134.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALTPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            complement(4408..5298)
                     /gene="rbsB"
                     /locus_tag="ACHKW0_003354"
     CDS             complement(4408..5298)
                     /gene="rbsB"
                     /locus_tag="ACHKW0_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HFO7476135.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            complement(5323..6288)
                     /gene="rbsC"
                     /locus_tag="ACHKW0_003355"
     CDS             complement(5323..6288)
                     /gene="rbsC"
                     /locus_tag="ACHKW0_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HFO7476136.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            complement(6293..7798)
                     /gene="rbsA"
                     /locus_tag="ACHKW0_003356"
     CDS             complement(6293..7798)
                     /gene="rbsA"
                     /locus_tag="ACHKW0_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HFO7476137.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGLGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            complement(7806..8225)
                     /gene="rbsD"
                     /locus_tag="ACHKW0_003357"
     CDS             complement(7806..8225)
                     /gene="rbsD"
                     /locus_tag="ACHKW0_003357"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HFO7476138.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            complement(8392..10260)
                     /gene="kup"
                     /locus_tag="ACHKW0_003358"
     CDS             complement(8392..10260)
                     /gene="kup"
                     /locus_tag="ACHKW0_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HFO7476139.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10483..11979
                     /gene="ravA"
                     /locus_tag="ACHKW0_003359"
     CDS             10483..11979
                     /gene="ravA"
                     /locus_tag="ACHKW0_003359"
                     /EC_number="3.6.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HFO7476140.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEIDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            11973..13424
                     /gene="viaA"
                     /locus_tag="ACHKW0_003360"
     CDS             11973..13424
                     /gene="viaA"
                     /locus_tag="ACHKW0_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HFO7476141.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(13429..14421)
                     /gene="asnA"
                     /locus_tag="ACHKW0_003361"
     CDS             complement(13429..14421)
                     /gene="asnA"
                     /locus_tag="ACHKW0_003361"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004071 - aspartate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HFO7476142.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EERLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            14573..15031
                     /gene="asnC"
                     /locus_tag="ACHKW0_003362"
     CDS             14573..15031
                     /gene="asnC"
                     /locus_tag="ACHKW0_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HFO7476143.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            15121..15564
                     /gene="mioC"
                     /locus_tag="ACHKW0_003363"
     CDS             15121..15564
                     /gene="mioC"
                     /locus_tag="ACHKW0_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HFO7476144.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            15943..17832
                     /gene="mnmG"
                     /locus_tag="ACHKW0_003364"
     CDS             15943..17832
                     /gene="mnmG"
                     /locus_tag="ACHKW0_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HFO7476145.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            17896..18519
                     /gene="rsmG"
                     /locus_tag="ACHKW0_003365"
     CDS             17896..18519
                     /gene="rsmG"
                     /locus_tag="ACHKW0_003365"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HFO7476146.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         4697 a         4870 c         4629 g         4527 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagagg aaatttaaaa taattttctg
      121 accgcgcatt ttttcagcaa aagccccgtt aatgtgccat aaaacaagca atttgccgtt
      181 tttgcaaccg taatcacact tcctggtggc atactaagga ggaataaaaa gaaaaaggaa
      241 gattagtaat gcctttaagc gcacaacagt tagctgcaca gaaaaacctt tcgtatgttc
      301 tggctgagaa gctggcgcaa cgcatcttaa aaggtgaata tgaacccggc accattttgc
      361 ctggtgagat tgagctgggc gagcaatttg gagtgagtcg tacagcggta cgcgaagcgg
      421 tcaaaacgtt aacggcaaaa gggatggttt taccgcgacc gcgaattggt acccgggtca
      481 tgccacaatc aaactggaat tttcttgatc aggaattgct tacctggtgg atgacagaag
      541 agaactttca tcaggtcatc gatcactttc tggttatgcg tatctgtctg gagccgcaag
      601 cttgcctact ggctgcaacg gttggcaccg cggaacagaa ggcgcatctc aatacgttaa
      661 tggccgaaat ggcggcatta aaagagaact ttcgccgtga acgctggatc gaagtcgata
      721 tggcctggca tgagcatatc tatgaaatga gcgccaatcc gtttttgacc tcattcgcct
      781 cgctattcca ttcggtttat cacacttact tcacgtcaat taccagcgac acagtgataa
      841 agctggatct gcaccaggcg attgtcgatg cgattgtcca aagcgatggc gacgcggcat
      901 ttaaagcttg tcaggcgctg ctacgctcac ctgataagtg acaacccgat aacaggacat
      961 tgaatgagcg ataaaaagaa gcgcagtatg gcgggtttgc cgtggatcgc ggcgatggcc
     1021 ttcttcatgc aggcacttga tgccactatt ctgaataccg ccttacccgc aatcgctcat
     1081 agccttaatc gttctcctct cgcgatgcaa tcagccatca tcagttatac gctgacggtg
     1141 gcgatgctta ttccggtaag cggatggcta gccgatcgct tcggtacgcg tcgcattttt
     1201 acccttgccg tgagtctgtt cacattgggt tctctggcct gcgcactttc taattcgcta
     1261 ccacagctgg ttgtcttccg ggttattcag gggataggcg gcgcaatgat gatgcctgtt
     1321 gctcggctgg ccttactgcg cgcttatcct cgtaatgaac ttcttccagt attgaatttt
     1381 gtcgccatgc cgggtctggt ggggccaatt ttaggccccg ttcttggcgg cgtgctggtc
     1441 acctgggcaa cctggcactg gatattttta atcaatatcc ccataggtat tgcgggcctt
     1501 ctttacgcgc gcaaacatat gcccaatttc accaccgcac gacgcagatt cgatatcact
     1561 ggctttttgc tgtttggcct cagccttgtt ctcttctcaa gcggaataga gctattcggg
     1621 gaaaggattg tcgccagctg gattgccttg acggtaattg tcaccagcat cgggttactg
     1681 cttctctata ttctccatgc gcgacacacg ccaaacccat taatttcatt agatttattt
     1741 aaaacccgca ctttctcgat cggtatcgta ggcaatattg caacccgtct ggggaccggt
     1801 tgtgtaccgt tccttatgcc attgatgtta caggtaggat ttggttatca ggcgtttatt
     1861 gccggctgta tgatggcacc gacagcgtta ggttccatta ttgcaaaatc gatggttacc
     1921 caagtcttac gtcgtctggg ctatcgccat acgttagtgg ggatcacggt gattattggg
     1981 ctaatgatcg ctcagttctc tttgcaatca ccggcaatgg cgatatggat gctgatcttg
     2041 ccgttgttta tattagggat ggctatgtcg acgcaattta ccgcgatgaa taccatcaca
     2101 cttgccgatc tgaccgatga caacgccagc agcggtaaca gtgttctggc ggtcacgcag
     2161 caactgtcga ttagtttagg cgttgctgta agtgcggccg tccttcgcgt ttatgaaggg
     2221 atggaaggca caacgactgt cgaacaattc cactatacgt ttatcacgat gggcattatt
     2281 actgttgctt cagcagcaat gttcatgctt ctgaaaacaa ccgatggtaa taatttgatc
     2341 aaaagacagc gtaaatctaa gccgaaccgc gttccatcag aatcggagta agttgtaatc
     2401 gttgttgctg aagggtcggc tgggttatcc gatggatgag tacatcaatc gccagctccc
     2461 ccagttcatc tttcggttgg tggatagtgg ttaatggtgg cgtcataaag cttgccagtt
     2521 cgatatcgtc atagccaatc accgcgatat cctgcggaac ctgtaactct gcctgatata
     2581 acgcctggta aacgccaaca gccatagcgt catttccggt aaagacggcc tgaggacgca
     2641 gcggatgtga taacagttgg cgcatggcgt caaacccgcc gttaaattca aaatcaccag
     2701 tgacttcata gccatcagga atgttgagac ccgcacgttt catcgccgcc cgataacctt
     2761 ccaaccgcag gcgcgccgga gttttatcca gcggaccggt aatacaggcg atacgggtat
     2821 gacctttatc gatcagatat tgcgttgcta agtctccgcc cagcaacgag ttatcctgaa
     2881 taagatcgct gtcgccatcg aacggagccc agtccatcat cacagtaggc actgtcggat
     2941 aacgttgcat gatttcacgc gaaggttgat gcgtttcggt gcacagtaac agcaagccat
     3001 caacgcgttt ttgcatcagc gtttccagat tgcgattcat ccgctgttca tcgccttcgg
     3061 tattgcaaag gacgagacta taaccgcgtt cgaagcagct gcgttcaacg ccacgcacca
     3121 gttctgaata gaaaggattg gtactggcag tgatcaacat gccaatggta tgtgtttgat
     3181 tgagtttgag gctacgcgcc agggctgatg gcgcgtaatt gagttcttta atcgccgctt
     3241 caactttggc ggtaatcgct tcactgacga agcgatcttt attgataacg tgagaaactg
     3301 ttgaggtaga aacgcccgcc aggcgggcaa catctttcat tgtagccaag cgtcacctct
     3361 gcctgtctaa aaatgcgtcg atctcttcac gccacggtac ggaaggttgt gcgcctttac
     3421 gtgttacggc aatcgcagcg gcagcatggg caaaccgaat cgcctctggc aatggttttt
     3481 cttccagcaa cgccgtgatt aacgcgccgt taaaggtatc tccggcagca atggtatcga
     3541 cagcctgcac ccggaatcca ggaacgcgct gaccttcacc attcacgcta gcccatacac
     3601 cacgacttcc taaagtaatc agtacagtac ggataccttt ttcatgcagt acctgcgccg
     3661 ccttcgctgc atcttcatca ttttcaacac gaataccggt gagcttttct gcttccgttt
     3721 cgtttggcgt aataatgtcc accagcgcca gcagttcgtc aggaagttcg cgagccggag
     3781 ccgggttaag cgcaacgata gtcttatttt gatgggcgat tttcgccgct gccatcacac
     3841 tttcgagtgg tgattccagc tgcattaata atgctgacgc gttggcaata cgctcacgtt
     3901 gcgcttccac cagcgccggg gtaagggcag cattagcgcc ggcatgaata ccgatgacat
     3961 tctcaccttc gccattaaca aaaatcagcg ccacacctgt tgattcgcct ttgatcacgc
     4021 tgaccggagt aatatcaatg ttatcagtgg cgagctgctg gcgaacgctc tcaccaatgc
     4081 tgtcatcacc cgtacaggca ataaacgcga tattcgcacc gctacgccca gcagccacag
     4141 cctgattcgc gcctttgccg ccaaatgcaa cctgatagtg gttaccggtt acggtttcgc
     4201 ctggagtagg aaaagattga agattaagaa tgtggtcagc attaatgctg ccaagaacaa
     4261 cgaggctgcc tgcgttttgc atattcggga tgtccataaa atgcgccacc gtgttagggt
     4321 ggcgcgtgcc ctgcttttct ttatattaca cgttcatctg tcgatgacgt atttatgtca
     4381 ccatcaggtc atacaacctg attaaaacta ctgcttaaca accagtttca gatcaaccgg
     4441 atacttagcc tgaactttct cgcctttcag cactttatct gcggtttcga cgcctttcgc
     4501 gccaatctga tcgggtagct gagcgatagt cgctgctagt ttgccatcat tcaccgcttt
     4561 ttcgccatcc ggtgtaccgt caaatccgac gaccatcaca tccgatttac cggcagtttg
     4621 cagtgcgcgc agcgcgccca gcgccatttc atcattctgc gcgaatacag cctgaacatc
     4681 cggatgagcg gtcaacaggt tctgcattac gttcaaacct ttagtgcgat caaaatctgc
     4741 tggctggctg gcaagaacat taaacttgtg agcagcaacg gcctgctgga agccttcgcc
     4801 acgttcacgg gctgcggatg taccagcaat gccttgcagc tcgataactt tggcaccttc
     4861 acccgctttc ttcgcgatgt aatcaccggc gattttgcca cccagtacgt tatcagaagc
     4921 aatgtggctc accacttcac ctttcgttgc ctggcggtca agagtgataa ccgggatgtt
     4981 cgcctggtta gccatcttca cagcattacc tactgcgtcg gagtcggtcg ggttaatcag
     5041 cagaattttg gtgccgcgaa cggttaagtc ctgcacgttc gccagctctt tcgccgggtt
     5101 gttctgggag tccagcacca ccaggttata gccaagctta tccgcctctt tctgcgcgcc
     5161 atctttcagc gatacaaaga acgggttgtt aagcgtggag accaccagcg cgatggtgtc
     5221 ttttgccatc gcattcgcac tgacggtggc gcttagcgca acagcggaaa ccagggtagc
     5281 cagttttttc atgttcatat tcaagatgtc ctgtagtcgt tattactgct ttttgttgtc
     5341 taccagcacc gccagcaaaa tcaccaccgc tttgacgatc atctggtaat aggaggaaac
     5401 acctaacaaa ttcaatccat tattaaggaa gccaagaatt aatgcgccga tcaacgtccc
     5461 aacaatgcga ccttttccgc ccgccagact cgtaccgccc agaaccaccg cagcaatagc
     5521 atccagctca tagccagtcc ccgccgtggg ttgtgcggag gagagacgcg ccacttcaat
     5581 gatcccggcc agcgatgcca gcagaccaca aagagaatag acgatgattt tgattttatt
     5641 gacgttgata ccagaaagac gcgttgctgc ttcgttgccg cccagcgcgt agatgtaacg
     5701 ccccagacgc gtgtgatgca gcatgtacca ggccgcgagg aagacaatcc ccatgatcca
     5761 gactggcgtc ggtacgccca gcggacgacc aataccaaac cagccaaaca gatcggcgtt
     5821 ctcagtaaag ccggtattca ctgggctacc gttggtataa accatggtca cgccgcgcag
     5881 taaaagcatc ataaccagcg tagcgataaa cgcctgaacg cgacctttcg ctacaatcac
     5941 cccggttacc gcaccaattg cggcacctaa cgcgagagca gcagcgacag ccaccagcgc
     6001 attgacttca atgccgacga tagatgcagc aactgcgccg gttagcgcca acagagaacc
     6061 tacagacaag tcgatgcccg acgtcaggat caccagcgtc atcccgaccg ccataatggc
     6121 gttcaccgag gtttgctgga gaatattgaa taagttattg atggtgaaaa agttcgggct
     6181 taacgtcgag acaatcgcga tcagcaccag cagagcgata agcgatttct gctccatcag
     6241 ccacgctttc gtgaaataac ggcgaccaga gacagtctgg gttgtcattt ttttactcct
     6301 gattcacgcg attaagcttg cccacagcgg cagccattaa cacttcctgg gtggcctgct
     6361 cacgagtaaa ttccccgctg agatgccctt catgcatgac gatgatgcga tcgctcatgc
     6421 ctaatacttc tggcatctcc gatgacacca gaatgatgct caagccatcg gctttgaact
     6481 ggttaatcag ttgatagatc tcttttttcg cgccgacatc tacgccacgg gtaggttcat
     6541 caaggatcaa cactttgggg cgtgtcatca gaccgcgggc aatcgccact ttttgctgat
     6601 tgccaccgga aagcagacca attgcctgtt ccatcgacgg tgttttcaca ttaaacagac
     6661 gaatgaaatc actcacagcc tgctgttcat cggcatgctt caaactgccg ccagcgcggc
     6721 tgaagtagcg cagcgctgtc agcgacatgt tctcttttac tgacatgccc aacactaaac
     6781 cgtcacgttt acggtcttcg gagatataca caatgccgct tgccaggcca tcctgcggtg
     6841 aacgggtaac gacttcatgc ccatccaggg tgacgtaacc gctggtgcgc ggtagtgcgc
     6901 cgtagagcac tttcatcagt tcggtacgac ctgcgcccat caaaccagag acgccaagaa
     6961 tttcgccttt gcgtaaagta aaagagacat cgttaacgcc aagtccgcag agattatcga
     7021 ctttcaggcg gatatctccc ggcgctttgt ccaggtgtgg atattgatct tccagcttgc
     7081 ggcccaccat catctcaatc agcgaatctt cggtcagtga tgccacttcg cgctcagcaa
     7141 taaattgccc gtcacgaaaa acggtaacgt catcgcaaat ctcgaagatt tctttcatgc
     7201 ggtgggagat atagacaata ccgcggcctt gcgatttcag ctcgcgtatg acgcggaaca
     7261 gggattcggt ttcggtatcg gtcagcgcat cggtcggttc atccataatg atgactttcg
     7321 actcaaagct cagcactttg gcgatttcaa ccatttgctg gtcaccgatg gaaagatcgc
     7381 ccaccagctt gtcgctttta aagcgcaggt taagtttagc cagcaattta tccgcttcgg
     7441 catacatggt tttccagtca attttgccaa agcgattaac aaactcacga ccgaggaaaa
     7501 tgttttcggc aatggtcaac tgcgggatca ggttcagttc ctgatggata atcccaatcc
     7561 cggcttcctg ggaagatttc ggcccggtaa atgtcgtttc tttccccagc cataaaagcg
     7621 tgccggcatc gcgagcatag ataccagtaa gcactttcat catggtggat ttacccgcgc
     7681 cgttttcgcc caccagcgcc atcacgcggc ccggatagac atttaacgct gcgcccgaga
     7741 gggcttttac gcccgggaag gctttatcga tgcctttaag ctgaagtaat gcttccatga
     7801 cggcctcaga acgtcacgcc agcacagaga atgatattcg catacggaga acattctccg
     7861 ctgcgaatta ccgcctgact ttctgcggtt tgttgtttga attgttcatg cgtggtgtaa
     7921 cgaatttcaa tggtatttcc ctggtgtttt tgcagctgct caaggtgagt gagcaacgtt
     7981 tcgtggagtt gcggattatg ttgtttgatc tcttccgcga taatggcagc ctcgacctgc
     8041 atttcatttg tgacgacgcc cagcacctgc ataaaagaag gtacaccctg ggttaatgcc
     8101 atatcgatac gcgttgtact tttggggatg ggtaaaccag catcacacac caccagcgta
     8161 tcggtatgtc ccagacggga gatcaccgat gaaatatcag aattaagaac ggtgcctttt
     8221 ttcatttttt tctccatcag cgaaacgttt cgctgacccg cagtttaatc ctgactgagt
     8281 tgggaaaacc atcgtgacgt tacggaaatg tgatcaacct cgaaacgttt tacatggtga
     8341 ttaaccatga aaacaaaaac gcccccttat ggagaaagga ggcgtctggc gttagatttc
     8401 gacctgagta cccagttcga taaccctgtt tggcggaatt tcaaattgat ctggtgcacg
     8461 cagcgcatta cgttgcagca gcaagtacag cttgccgcgc agacgcaaat accacgggcg
     8521 tttgccgagg atcaacgact catgcgacat aaagaaggag gtttccatca tccggcaact
     8581 taatccttcc agaccgcagc ggtggaaaac ttcttccacg tttggcgttt ctcgccaacc
     8641 ataacttgcc accacgcgcc agaaagtggg cgacagttgt tcaatctgta cccgacggac
     8701 attatggaca tatggcgcgt cttcggtacg cagagttaac agaatcaccc gctcatgcaa
     8761 taccttgtta tgtttaaggt tatgcatcag cgcaaaggga atgacgttga ttgcacgcga
     8821 catatacacc gcggtcccgg gcacgcgaac aggcggtgat ttctccagcg aagcaatcat
     8881 cgcttccaga gagttaccat gttcatgcat ccgccgcagc aagcggaaac gctcgctctt
     8941 ccaggtggtc atcacgataa acatcacagt accgaggctc aatggcaacc agccgccgga
     9001 gagcagttta tcgaggttag cggtgaacaa tggaatatcg acacaaagga aagcaatcag
     9061 gatcagcgca acaaaatact tattccagtg ccagttctga cgtgccacgg tagtcgagag
     9121 aatagacgtc agcaccatgg ttccggtcac cgcaatcccg tacgccgccg ccaggttgct
     9181 ggagtgctca aagctgacaa tcacaatcac gaccgcgaca tagagcatcc agttcacaaa
     9241 cggaatatag atttgccctg actccatttc ggaggtgtga ataatgcgca tcggcgacaa
     9301 atatcccaga cgtaccgcct gacgcgtcaa tgagaagacg ccagagataa ccgcctgcga
     9361 ggcaattacc gtcgccagtg cggcgatgat cagcagcggg atcagcgccc agtccggtgc
     9421 caacaggaag aacgggttct taatcgcttc cgggttcttt aacaacagcg ctccctgacc
     9481 gaagtaatta agggtcaagg aaggtaatac gacggtgaac cacgccaggc gaatagggaa
     9541 cttaccaaag tgccccatat cagcatacag cgcctcgacc cccgtaatcg acaacaccac
     9601 tgcccctaat gcaataaaag aaaccgtttt gtattcaagg aagaaatgca ccgcccacat
     9661 tggattcagc gcatgcagca cttccgggtt agcaataatg ctacgtaacc ccagtcctgc
     9721 cagaatcaaa aaccaggtca gcatgatcgg cgcaaacagc ttaccgacca tagccgtgcc
     9781 atgtttttga atcataaata ataaagtgag aacgataatt gagaggggaa ctatccaggt
     9841 atccagctgc ggggcgacga tttccagacc ttcaatggct gacatcaccg aaatagcggg
     9901 tgttatgacg acttcaccat agaaaaagct gccgccgatt agccccataa tcaccagcat
     9961 tgatgtggtt cgcgccgacg tattacgccc ggcaagcgac atcaacgtca ggatcccccc
    10021 ctcaccggcg ttatctgccc gcatcacgaa ggtgagatat ttaatggaaa ccacaaagat
    10081 tagcagccag aagatcagcg ataaaaagcc aaacacggca tcgcgttcaa cgccaaaacc
    10141 aaactggccg gacaaacatt cacgaagtgt atataacggg ctggtaccaa tatcgccgta
    10201 gacaactcca atcgccgcga gggtaatcgc gggcaatgat tgcttattat cagtgctcat
    10261 agactagtct ttcgttgaaa tatgaaatgt gtgcttagtc ccttggccca caaaaaagcg
    10321 cacagtatgc acgattaacg gcaaaatcgt actcctaaat gcggccacat taacctggct
    10381 caaagaaaaa taccccccct ttgagactat tttagccacc agcaagaaac gtctatactc
    10441 gcaatttacg cagaactttt gacgaaagga cgccacttca ttatggctca ccctcattta
    10501 ttagcggaaa gaatttcccg cctgagcagt tcgttggaaa aggggcttta tgaacgtagc
    10561 cacgccatcc gcttgtgttt attagcggca ttaagtggtg aaagtgtgtt cctccttggc
    10621 ccgccaggta ttgccaaaag tttgatcgcc cggcgcttaa aattcgcctt tcagaatgcc
    10681 cgcgcgtttg aatatctgat gacccgcttc tccacgccgg aagaagtttt tggtcccctt
    10741 tctattcagg cgctaaaaga tgaagggcgc tatgaacgtt taaccagcgg ttacctgccg
    10801 gaagcagaaa ttgtctttct ggatgagatc tggaaagcgg gcccggcaat tcttaatacc
    10861 ttgctcaccg ccattaacga gcgccagttc cgcaacggtg cacacgtaga aaaaatcccg
    10921 atgcgtctgc tggtggcggc ctccaacgag ctgccggaag cagacagcag tctggaagcg
    10981 ttatatgacc gcatgctgat tcgtctgtgg ttagataaag tgcaggataa agcgaatttc
    11041 cgctccatgc tgaccagtca acaggatgaa aacgacaatc cggttcctga tgccttgcag
    11101 gtcacagatg aagaatatga acgctggcag aaagagattg gtgaaattac gctgcccgat
    11161 catgtatttg agctgatttt tatgctgcgc cagcaactgg ataaattacc ggatgcgcct
    11221 tatgtctcgg atcgtcgctg gaaaaaagcg atccgattat tgcaggccag cgcctttttt
    11281 agcggtcgca gtgctgttgc cccggtagat ctcattttgc tgaaagattg cctgtggtat
    11341 gacgcgcaaa gcctgaattt gatacaacaa caaattgatg tattgatgac cggtcacgcc
    11401 tggcaacagc aagggatgtt gactcgcctt ggcgcgattg tgcaacgtca cctgcaactc
    11461 cagcagcaac aaagcgataa aacagcctta acggtaattc gtctgggcgg cattttcagc
    11521 cgtcgtcagc agtatcaact tcctgttaac gttactgctt ccactctgac tctactgctg
    11581 caaaaaccgt taaaactgca tgatatggaa gtggttcata tctcctttga gcgtagcgcg
    11641 ctggaacagt ggctgagcaa aggtggtgaa attcgcggta aactgaacgg tatcggcttt
    11701 gcccagaaac tgaatctgga aattgatagc gcccaacatc ttgtcgttcg cgatgtaagt
    11761 ttacaaggca gtacgctggc acttcccggt tcatcggctg aaggtctgcc tggtgaaata
    11821 aaacaacaac tggaagagct tgaaagcgac tggcgcaagc aacacgcttt attcagcgaa
    11881 cagcaaaaat gtctgtttat ccctggcgac tggttaggtc gcattgaagc cagcctacag
    11941 gatgtcggtg cacagattcg ccaggcacaa caatgctaac gctggatacg cttaatgtga
    12001 tgctggccgt cagcgaagag ggattgatcg aagagatgat catcgcactg ctggcctcac
    12061 cgcagctggc ggtcttcttt gaaaaattcc cacgcctgaa ggcggcaatc actgatgatg
    12121 ttccccgctg gcgtgaagcg ctgcgcagtc ggctgaaaga tgcccgagtc ccgccggaac
    12181 tcaccgaaga ggtgatgtgc tatcagcaaa gccagctcct ctccacgcca cagtttattg
    12241 tgcagctacc acagatcctg gacttactgc atcgtctgaa ttctccatgg gcagaacaag
    12301 cccgacagtt ggttgatgct aacagcacga tcacttcagc gttacacacg ctttttctcc
    12361 agcgttggcg tttaagtctg atcgtgcaag caacgacgtt aaatcaacag ctattagaag
    12421 aagaacgcga acaactgttg agtgaagttc aggaacgcat gacgctgagc ggacaacttg
    12481 aaccgattct cgcagataac aatactgcag ctggtcgtct gtgggatatg agcgccggtc
    12541 agcttaaacg tggcgactat cagttgattg tgaaatacgg tgaatttctt aacgaacagc
    12601 cggaactgaa acgcctggca gagcagctgg ggcgttctcg ggaagccaaa tcaataccgc
    12661 gcaacgatgc gcagatggaa accttccgca ccatggtgcg cgaaccggcg acggttcctg
    12721 agcaggttga tggtctgcaa caaagcgatg atattttacg tcttctgccg ccagaactgg
    12781 cgacactagg gataacggaa ctggagtatg agttttaccg tcggctggtg gaaaaacagt
    12841 tgctcaccta tcgcctgcac ggtgagtcgt ggcgtgaaaa agtgatcgaa cgtccggtgg
    12901 tacataaaga ttacgatgaa cagccgcgcg ggccgtttat tgtctgtgtg gatacttccg
    12961 gctcaatggg cggctttaat gaacagtgtg cgaaagcgtt ctgcctggcc ttgatgcgca
    13021 ttgctctcgc agaaaaccgg cgctgctata ttatgctatt ttccaccgag atcgtccgtt
    13081 atgagctttc aggcccacaa ggcatcgaac aagcaatccg ttttttaagc cagcagtttc
    13141 gtggcggcac cgatcttgcc agttgttttc gcgccattat ggaacgcttg caaagcaggg
    13201 aatggtttga tgccgatgcg gtggtgattt ctgattttat cgctcagcgg ttgcctgacg
    13261 acgtgacgag taaagtgaaa gagctgcagc gggtacatca gcatcgcttt catgccgtgg
    13321 cgatgtcggc acacggcaaa cccggcatca tgcgcatttt cgatcatatc tggcgctttg
    13381 ataccgggat gcgaagccgc ctgctcagac gctggcggcg ataaattatt acagcagaga
    13441 agggacgctc tcgcgaacag cagctggcca tactccacac tgaacctggc cgatatgcgg
    13501 cagttgcagc agcagcatgg tcaaacgaga ctggccgata ccgccgccga tggtctgcgg
    13561 catttcaccg cgcagcagcg cctgatgcca ttccagctgc agacgatctt catcaccggt
    13621 cagcgccaac tgatgcttca acgtgtcggc atccacgcgg atccccatgg aagaaagctc
    13681 aaacgcatct tccagtacag ggttccatac cagaatatcg ccgttcagac ccgcatggcc
    13741 cagctctgac ggggtgctcc agtcatcata atccggtgcg cgcacgtcgt gacgatgacc
    13801 atcactcagc ttgccgccaa tcccgacgag gaataccgcg ccaagatctt tcgcaatcgc
    13861 ccgctcacgc cctttggcat cgagatccgg ataacgagac agtaactcct ggctgtgtac
    13921 gaagtggatc tgatccggca ggaacggtgc caaaccaaac tcttcgctaa ctgcagcttc
    13981 ggttgcttta attcccgccc agatcgcctc taccgtgctt ttcagagtcg agaattgacg
    14041 ctcaccgtcg cccattacgc gttcccagtc ccactggtca acatagaccg agtgcaacgg
    14101 agaaagacgc tcttcatcgg ggcgaagggc tttcatgtgc gtgtacagcc cttcgcccgc
    14161 gctgaagtcg tgttgcccta aggtctgacg tttccacttc gccagtgaat gaaccacttc
    14221 gaactgggca tcaggcagag ctttcacttt tacctgcacc gctttttcac agcccgacaa
    14281 gttatcctgc gtgccatccc ccacacggct aagaatcggc gcctggactt cgatcagccc
    14341 cagacgttct tccagttggc gagaaaagtg agatttcacg aagctaattt ggcgttgttt
    14401 ggcaatgtaa gcggttttca ttttttatac tcctgcgtcc tgttgcttat gattaagcaa
    14461 caaaaaccga cacacatgca ataatcattc aataaaaagc ctaacacact tttgattcat
    14521 taaaaaagaa ggctaaaata gaatgaatca tcaatctgca taagaaaatc ctatggaaaa
    14581 ttatctgatc gacaatctgg accgtggcat cctggaagca ttaatgggca atgcgcgcac
    14641 cgcttacgcc gaactggcga aacaatttgg cgtcagtccg gggacgattc acgttcgagt
    14701 agagaaaatg aagcaggcgg ggatcattac cggggcgcgt attgatgtca gcccgaagca
    14761 gctcggttat gacgtaggct gctttatcgg cattatatta aagagcgcca aagactaccc
    14821 ttccgcgctg gcaaagctgg aaagccttga tgaagtcact gaagcctatt acacaaccgg
    14881 ccactacagc atctttataa aagtaatgtg ccgttcgatc gacgctctcc agcatgtact
    14941 tatcaacaag atccaaacaa ttgatgaaat tcagtccacc gagacattga tcgtcctgca
    15001 aaacccgatc atgcgtacta tcaagccctg atcggctttt ttaatcccat acttttccac
    15061 aggtagatcc caacgcgttc acagcgtaca atacgccact cttaataaag gtggcggttt
    15121 atggcagata tcactcttat cagcggcagc accctcggcg gtgccgaata tgtagcagaa
    15181 cacctggctg aaaagctgga agaggcgggt tttaccaccg aaacgctgca cggtccgctg
    15241 ttggaagatt tatctgcctc ggggatctgg ctgattatca gctccaccca cggtgccgga
    15301 gatattccgg acaacctttc tcctttctat gaagcattgc aggaacagaa gcccgatctt
    15361 tctgcggtcc gctttggcgc aatcggtatt ggtagtcgtg aatatgacac cttttgtggg
    15421 gctatcgata aactcgaggc agaactcaaa aattccggtg caaaacagac aggcgaaaca
    15481 ctgaagatca acattcttga tcacgacatt ccggaagatc cggcagaaga atggctggga
    15541 tcgtggatta atttactcaa ataagtatac agatcgtgcg atctactgtg gataactctg
    15601 tcaggaagct tggatcaacc ggtagttatc caaggaacaa cggttgttca gtttttgagt
    15661 tgtgtataac ccctcattct gatcccagct tatacggtcc aggatcaccg atcattcaca
    15721 gttaatgatc ctttccaggt tgttgatctt aaaagccgga tccttgttat ccacagggca
    15781 gtgcgatcct aataagagat cacaatagaa cagatctcta aataaataga tcttcttttt
    15841 aatacccagg atcccaggtc tttctcaagc cgacaaagtt gagtagaatc cacggcccgg
    15901 gcttcaatcc attttcatac cgcgttatgc gaggcaatca ccatgtttta tccggatcct
    15961 tttgacgtca tcatcattgg cgggggtcat gcaggcaccg aggccgcgat ggccgcggcg
    16021 cgtatgggtc aacagactct gcttttgaca cacaatatcg acactctggg gcagatgagc
    16081 tgcaacccgg cgatcggcgg tattgggaag ggacatctgg taaaagaagt ggatgcactc
    16141 ggcggtctga tggcgaaagc gatcgatcaa gcgggtatcc agtttaggat actaaacgca
    16201 agcaaaggac cggcggttcg cgctacccga gctcaggcgg atcgtgtgct ctaccgtcag
    16261 gcggtacgta cggcgctgga gaaccaaccg aacctgatga tcttccagca ggcggttgaa
    16321 gatcttattg tcgaaaacga tcgcgtggtc ggtgctgtta cccaaatggg actgaagttc
    16381 cgtgccaaag ccgtcgtgct caccgttggg acgttcctcg acggtaaaat tcatatcggt
    16441 ctggataatt acagcggtgg ccgtgctggt gatccgccgt ccattccgct ttctcgccgt
    16501 ttgcgtgaac tgccgctgcg cgttggtcgt ctgaaaaccg ggacaccacc gcgtattgat
    16561 gctcgaacta tcgactttag tgtgctggcg caacagcatg gcgataaccc aatgccggta
    16621 ttctcgttta tgggcaatgc gtcccagcat ccccagcagg tgccgtgtta tatcactcat
    16681 accaacgaga aaacccatga tgtgatccgc agtaacctcg atcgtagccc aatgtatgca
    16741 ggggtgatcg aaggtgtcgg cccacgctac tgcccgtcga tcgaagacaa agtcatgcgc
    16801 ttcgccgaca gaaatcagca tcagatcttc cttgaaccgg aagggctgac ttctaacgaa
    16861 atttatccga acggtatctc caccagcctg ccgttcgatg tgcagatgca aatcgtccgc
    16921 tctatgcagg ggatggaaaa cgcgaagatc gtgcgtccgg gttatgccat tgagtatgac
    16981 ttcttcgatc ctcgcgacct gaaaccgacg ctggagagca agtttatcca ggggctgttc
    17041 tttgctggtc agattaacgg cactaccggt tacgaagaag ccgctgcgca aggtttgctg
    17101 gccggtctta acgctgcccg tctgtctgct gacaaagaag gttgggctcc ggcgcgttct
    17161 caggcctatc tcggcgtact ggttgatgac ctgtgcactt taggaaccaa agaaccgtat
    17221 cgtatgttta cttcgcgcgc agaatatcgt ctgatgctgc gcgaagataa tgcggatctg
    17281 cgtttgactg aaatcggtcg tgaactgggc ctggtggatg acgaacgttg ggcgcgcttt
    17341 aacgagaaac ttgagaatat cgagcgtgag cgtcagcgtc tgaaatcgac ctgggtaacc
    17401 ccgtcggcgg aagctgcagc cgaagtgaat gctcacctga ctgcgccgct ttcccgtgaa
    17461 gccagtggtg aagatctgct gcgtcgtccg gaaatgactt atgaaaaatt aaccacgctg
    17521 acgccgtttg cccctgcgtt gacagacgaa caggcggcgg aacaggttga gattcaggtt
    17581 aaatacgaag gttatatcgc gcgccagcaa gatgagatcg aaaagcagct gcgtaacgag
    17641 aacaccctgc taccagcgac actggattac cgccaggtat ccggtctttc taacgaagtg
    17701 atcgccaaac ttaacgatca caaaccggcc tctatcggcc aggcttcacg tatttctggc
    17761 gtcacgcctg cggccatctc cattctgctg gtgtggctga aaaaacaggg tatgctgcgt
    17821 cgtagcgcat aacgcattaa aaatgcctgg taagcacccg cttaccaggc aacgcatcaa
    17881 gaacaggtaa tcaccgtgct caacaaactc tccttactgc tgaaagacgc aggtatttcg
    17941 cttaccgatc accagaaaaa ccagcttatt gcctacgtga atatgctgca taaatggaac
    18001 aaagcgtaca acctgacttc ggtccgcgat cctaatgaga tgctggtacg ccatattctc
    18061 gatagcattg tggtggcacc gtatctgcaa ggtgaacggt ttatcgatgt cggcaccggt
    18121 cctggactgc ctggcattcc actctctatc gtgcgtcctg aagcccattt cactctgttg
    18181 gatagccttg gtaaacgcgt gcgtttcctt cgtcaggtgc aacatgagct taaactggag
    18241 aatattgagc cagtacagag cagggtagaa gagtttcctt cagagccgcc atttgatggc
    18301 gtaattagcc gcgcttttgc ctctctgaac gatatggtga gctggtgtca ccatcttcct
    18361 ggtgagcaag gccgtttcta cgcgctgaaa gggcaaatgc cggaagatga aatcgctttg
    18421 ttgcccgaag aatatcaggt tgaatcagtg gttaaacttc aggttcctgc cctggatggc
    18481 gaacgccatc tggtggtgat taaagcaaat aaaatttaat ttttatcaaa aaaatcataa
    18541 aaaattgacc ggttagactg ttaacaacaa ccaggttttc tactgatata actggttaca
    18601 tttaacgcca cgttcactct tttgcatcaa caagataacg tggctttttt tggtaagcag
    18661 aaaataagtc attagtgaaa atatcagtct gctaaaaatc ggcgctaaga atcatcattg
    18721 gct
//
LOCUS       DAZFXT010000090        18549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.090, whole genome shotgun sequence.
ACCESSION   DAZFXT010000090 DAZFXT010000000
VERSION     DAZFXT010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.090"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..171
                     /locus_tag="ACHKW0_003366"
     CDS             <1..171
                     /locus_tag="ACHKW0_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476147.1"
                     /translation="TTTAATTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGG
                     SHNTSTTTSTTR"
     gene            278..922
                     /gene="gfcB"
                     /locus_tag="ACHKW0_003367"
     CDS             278..922
                     /gene="gfcB"
                     /locus_tag="ACHKW0_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HFO7476148.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLHLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRDV
                     LCNVWQEEVTSTLPEKQWQNIFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            919..1665
                     /gene="gfcC"
                     /locus_tag="ACHKW0_003368"
     CDS             919..1665
                     /gene="gfcC"
                     /locus_tag="ACHKW0_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC D2 domain-containing
                     protein"
                     /protein_id="HFO7476149.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLHITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGTG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            1665..3761
                     /gene="gfcD"
                     /locus_tag="ACHKW0_003369"
     CDS             1665..3761
                     /gene="gfcD"
                     /locus_tag="ACHKW0_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7476150.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEPLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            3807..4946
                     /gene="gfcE"
                     /locus_tag="ACHKW0_003370"
     CDS             3807..4946
                     /gene="gfcE"
                     /locus_tag="ACHKW0_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HFO7476151.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTANADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKIFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            4934..5380
                     /gene="etp"
                     /locus_tag="ACHKW0_003371"
                     /gene_synonym="yccY"
     CDS             4934..5380
                     /gene="etp"
                     /locus_tag="ACHKW0_003371"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HFO7476152.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            5400..7580
                     /gene="etk"
                     /locus_tag="ACHKW0_003372"
     CDS             5400..7580
                     /gene="etk"
                     /locus_tag="ACHKW0_003372"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HFO7476153.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDMVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLDWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(7700..8998)
                     /gene="appA"
                     /locus_tag="ACHKW0_003373"
     CDS             complement(7700..8998)
                     /gene="appA"
                     /locus_tag="ACHKW0_003373"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HFO7476154.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSESELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQP
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSKLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9074..9166)
                     /gene="cbdX"
                     /locus_tag="ACHKW0_003374"
     CDS             complement(9074..9166)
                     /gene="cbdX"
                     /locus_tag="ACHKW0_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HFO7476155.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(9179..10315)
                     /gene="appB"
                     /locus_tag="ACHKW0_003375"
     CDS             complement(9179..10315)
                     /gene="appB"
                     /locus_tag="ACHKW0_003375"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HFO7476156.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(10327..11871)
                     /gene="appC"
                     /locus_tag="ACHKW0_003376"
     CDS             complement(10327..11871)
                     /gene="appC"
                     /locus_tag="ACHKW0_003376"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HFO7476157.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12005..12862)
                     /gene="hyaF"
                     /locus_tag="ACHKW0_003377"
     CDS             complement(12005..12862)
                     /gene="hyaF"
                     /locus_tag="ACHKW0_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HFO7476158.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQV
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(12859..13257)
                     /gene="hyaE"
                     /locus_tag="ACHKW0_003378"
     CDS             complement(12859..13257)
                     /gene="hyaE"
                     /locus_tag="ACHKW0_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HFO7476159.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMISELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            complement(13254..13841)
                     /gene="hyaD"
                     /locus_tag="ACHKW0_003379"
     CDS             complement(13254..13841)
                     /gene="hyaD"
                     /locus_tag="ACHKW0_003379"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HFO7476160.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            complement(13838..14545)
                     /gene="hyaC"
                     /locus_tag="ACHKW0_003380"
     CDS             complement(13838..14545)
                     /gene="hyaC"
                     /locus_tag="ACHKW0_003380"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HFO7476161.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLLLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            complement(14564..16357)
                     /gene="hyaB"
                     /locus_tag="ACHKW0_003381"
     CDS             complement(14564..16357)
                     /gene="hyaB"
                     /locus_tag="ACHKW0_003381"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HFO7476162.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(16354..17472)
                     /gene="hyaA"
                     /locus_tag="ACHKW0_003382"
     CDS             complement(16354..17472)
                     /gene="hyaA"
                     /locus_tag="ACHKW0_003382"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HFO7476163.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            17899..17986
                     /locus_tag="ACHKW0_003383"
     tRNA            17899..17986
                     /locus_tag="ACHKW0_003383"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17933..17935,aa:Ser,seq:tga)
     gene            18075..18264
                     /locus_tag="ACHKW0_003384"
                     /pseudo
     CDS             18075..18264
                     /locus_tag="ACHKW0_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346438.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspV"
BASE COUNT         4539 a         4929 c         4792 g         4289 t
ORIGIN      
        1 accacgacgg ctgctaccac tggtggcgtg gctgctggcg ctgtcggcac ggcgacagtt
       61 gtcggtgtgg caacggctgt tggtgtggcg acgttggcag tagttgcagc aaatgacagt
      121 ggcgatggcg gttctcataa cacctcaacc accacgtcca ctacgcgcta attctgctta
      181 aacgcgtcga aattaaagtt aaataatacc cggttgcgat attcgtaccg ggctttagag
      241 atttgcattt ttttaattaa cctttcaagg attaaaggtg cgccctctta ttttatcgat
      301 tttcgcacta tttcttgcgg gatgtacgca cagccagcaa agtatggtcg atacatttcg
      361 cgccagcctt ttcgataatc aggatatcac cgtagcggat cagcagatcc aggcgttgcc
      421 ttattccacg atgtatttac accttaatga agggcaacga atctttgtgg tactgggata
      481 tatagaacaa gaacaaagca aatggttatc ccaggataac gccatgctgg ttacccataa
      541 tggacgtctt ttaaagaccg tcaaacttaa taataatctg ctggaagtga caaattccgg
      601 gcaggaccct ctgcgtaacg cgctggcaat aaaagacggc agccgctgga cgcgcgatat
      661 tctctggagt gaagacaacc attttcgctc tgcgaccctg agttccactt tttcctttgc
      721 tggattagag acgctgaata ttgcgggtcg cgatgtgctg tgtaatgtct ggcaggaaga
      781 ggtgacttcc acgctgccag aaaaacagtg gcaaaacata ttctgggtcg attcggctac
      841 tggccaggtt cgtcaaagtc gacaaatgtt aggcgcaggg gttattcccg tagaaatgac
      901 gtttcttaaa cccgcaccat gaataaatta cagtcgtatt tcattgccag cgtactttac
      961 gtaatgacac cccatgcctt tgcgcaagga acggtgacta tttatctgcc tggcgaacaa
     1021 cagacacttt ccgttggccc cgtggaaaat gttgtccagt tggtgacaca accgcaactg
     1081 cgcgatcgcc tctggtggcc tggtgccttg ctgaccgact ctgcggcgaa agctaaagcg
     1141 cttaaagact accaacatgt catggcacag cttgcttcct gggaagcaga agctgacgac
     1201 gatgtggcag caacgattaa atccgtgcgc cagcagttac tcaatctcca tatcactggt
     1261 cgcttaccgg tcaaactgga ccctgatttt gtacgagtgg atgagaacag caatcccccg
     1321 ctggtcgggg actatacgct ctataccgta cagcgtccgg tgactattac tctgctgggt
     1381 gcggtttctg ggactgggca attgccctgg caggcaggcc gtagcgttac cgattatctg
     1441 caagatcacc cccgccttgc aggcgcggac aagaataacg ttatggtgat tacgcctgag
     1501 ggtgagactg tcgtcgcccc tgttgccctg tggaataaaa gacatgtgga accaccgcct
     1561 ggcagccagc tttggttggg cttttctgcg catgtactgc ccgaaaaata tgctgattta
     1621 aatgaccaga ttgtgtctgt tttaacgcaa cgtgttcctg actgatgaag aagaattctt
     1681 atcttttaag ctgcctggcc attgccgtct ccagtgcctg tcatgctgaa gtattaacct
     1741 acccggatcc gctgggttcg tcgcaatcag actttggcgg cacaggattg ttgcagatgc
     1801 caaatgcgcg catcgcaccg gaaggtgaat tcagcgtcaa ctaccgggat aacgatcaat
     1861 accgtttcta ctccacctcc gtggcgctgt tcccatggct ggaaggcacc attcgttata
     1921 cggatgtgcg cacacgcaaa tatagccagt gggaagattt cagcggcgat cagtcataca
     1981 aagacaaatc tttcgatttt aaacttcgcc tatgggaaga aggttactgg ctaccgcaag
     2041 tagcgtttgg taaacgtgat attgctggta cgggtctgtt tgacggtgag tatctggtgg
     2101 ccagcaagca agcggggcca tttgatttca ccctcgggat ggcatggggc tacgccggta
     2161 atgcgggcaa tattaccaac ccgttttgcc gggtgagcga taaatattgt catcgcgcag
     2221 agtctcacga tgcgggcgat atcagcttta gcgatatctt tcgtggcccg gcttccatct
     2281 ttggcggcat tgagtatcaa acgccgtgga atcccctgcg tctgaaactc gaatacgacg
     2341 gcaacaatta ccagaatgat ttcgctggca aactgcctca ggcaagccat ttcaacgtcg
     2401 gtgcagttta tcgcgctgcc agctgggcag acctcaacct gagttatgaa cgcggtaaca
     2461 cgttgatgtt tggtttcact ttacggacca atttcaacga tctgcgccct gccctgcgcg
     2521 atacgccaaa accggcatat caacctgcgc ctgaatctga aggattgcag tacaccacgg
     2581 tagcaaacca acttaccgcc ctgaagtata acgcgggctt tgacgcgcca gaaattcagc
     2641 tacgcgataa gacgctgtat atgtctggtc agcaatacaa ataccgtgac tctcgtgaag
     2701 cggtcgatcg tgccaaccgg attctggtga ataacctgcc gcaaggcgtt gagaagatta
     2761 gcgtgacgca aaagcgcgag catatggcta tggtgactac cgaaaccgac gtagccagcc
     2821 tgcgcaaaca gctggccggt acagcgcctg gtcaatcaga gccactgcaa caacaacgtg
     2881 ttgaagctga agatctttct gcctttggtc ggggctaccg tattcgtgaa gatcgcttta
     2941 gctactcttt caacccaaca ctttcacagt cgctgggcgg cccggaagat ttctatatgt
     3001 tccagttggg gctgatgtcc agcgcccgct actggtttac tgaccacctg ctgcttgatg
     3061 gcggtatttt caccaatatt tacaataact acgacaagtt taagtcttcg ctattacccg
     3121 cggactctac cctgccccgc gtgcgcacgc atatccgtga ttacgttcgc aatgacgttt
     3181 atctcaacaa cttgcaggcg aactactttg ccgacttagg caatggtttc tatggccagg
     3241 tgtatggcgg ttatctggaa acgatgtacg ccggtgtcgg ttccgagctg ctttatcgcc
     3301 cgctagatgc cagctgggcg ctgggtgtgg acgttaacta cgtgaagcaa cgtgactggg
     3361 acaacatgat gcgcttcacc gattattcca cgccaacagg tttcgtgacg gcttactgga
     3421 acccgccgac gctcaatggc gtactgatga aacttagcgt tgggcaatat ctggcaaaag
     3481 ataaaggggc aacgatcgac gtcgccaaac gctttgacag cggcgtggcg gtaggggtat
     3541 gggcggcaat cagtaacgta tctaaagatg actacggcga aggcggcttt agtaaaggtt
     3601 tttatatctc gattccgttc gacttgatga ccattggacc taaccgcaac cgcgcggtgg
     3661 tttcgtggac accattgacg cgtgatggtg gacaaatgct gtcacgcaaa taccagctct
     3721 atccaatgac ggcagagcga gaagtaccgg ttggacaata acctgacgta attcataaca
     3781 ttacattgac tgataaatat aaaccaatga aaaagaacat ttttaagttt tcggtactga
     3841 cattggctgt tctgtctttg acagcatgta cccttgttcc tggtcagaat ttatcgacca
     3901 gtaacaagga tgtcattgaa ctacctgaca atcagtatga cctggacaaa atggtcaata
     3961 tctatcccgt cacgccagga ttgattgatc agctgcgcgc caaaccgatt atgtcgcagg
     4021 ctaacccgga gctggagcag caaatcgcga actatgaata tcgtatcggc attggtgatg
     4081 tgttgatggt tacggtctgg gatcacccgg aactaaccac ccccgcaggg caataccgta
     4141 gcgccagtga taccggcaac tgggttaacg ccgatggtgc gattttctac ccctatattg
     4201 gtcgcctgaa agtggccggg aaaacactta cccaggtacg caacgagatt acagcgcgtc
     4261 tcgattcagt cattgaaagc ccgcaggtcg atgtcagcgt cgcagcgttc cgatcgcaaa
     4321 aagcgtacgt caccggcgaa gtcagcaaat cgggtcagca gccgattacc aatattcctc
     4381 tgaccatcat ggatgcgatt aacgccgcgg gcggtctgac cgcaaatgca gactggcgca
     4441 atgtggtgct cacccagaat ggcgtaaaga ccaaagtgaa cctctatgcg ctgatgcagc
     4501 gtggcgactt gcgtcagaac aaactgctgc acccgggcga tattttattt attccgcgca
     4561 acgatgatct gaaaatcttc gtgatgggtg aagtgggtaa acagagcacg ctgaaaatgg
     4621 accgcagcgg tatgaccctg gcggaagcgc tgggtaacgc tgagggtatg aatcaggatg
     4681 tcgctgatgc gacggggatt tttgttattc gtgcaacgca aaacaagcag aatggaaaaa
     4741 tcgccaatat ttatcagctg aatgcgaagg acgcatcggc aatgatactc ggaacggaat
     4801 tccagcttga accttacgac attgtttatg tcactaccgc gccgctggct cgttggaacc
     4861 gtgtgatttc tctgctggta ccgacgattt ccggggtgca tgacctgaca gagacttcac
     4921 ggtggattca aacatggccc aactaaaatt taactcaatc ctggtggttt gtaccggcaa
     4981 catttgccgt tcgcccattg gcgagcgctt gctgcgtaaa cgtctgccgg gcgtaaaagt
     5041 caaatcggca ggtgttcatg gtctggtaaa acaccctgca gatgcgacag cggcagatgt
     5101 tgccgctaat catggcgtat ctcttgaagg acatgccgga cgtaagctca ctgctgagat
     5161 ggcacgaaat tacgatctga ttctggcaat ggagtcggaa catattgctc aggttaccgc
     5221 aatcgctccc gaggtccgcg ggaagacaat gctgtttggg caatggctgg aacagaaaga
     5281 gatcccggat ccctatcgta aaagtcagga cgcatttgaa catgtctacg gtatgttgga
     5341 gcgcgccagt caggaatggg cgaagcgcct cagccggtaa tttgagttta taaaatacga
     5401 tgacaactaa aaatatgaat acgccaccag gcagcactca ggaaaatgag atcgatctgc
     5461 ttcgtctggt cggcgagtta tgggatcacc gtaagtttat tatcagcgtg accgcgttat
     5521 tcacgctgat cgctgtcgct tactcgctgt taagcacacc aatttatcag gcagatactc
     5581 tggtccaggt tgagcaaaaa cagggcaacg ccattctcag cggcctgagc gatatgatcc
     5641 ctaactcatc gcccgagtct gcaccggaga tccaactgct gcaatcgcgc atgattctcg
     5701 gtaaaaccat tgctgaactg aatctgcgtg acatggttga gcaaaagtat tttccgattg
     5761 tgggtcgcgg ctgggcgaga ttaaccaaag agaaaccagg tgagctggcg atcagctgga
     5821 tgcatattcc acaactgaat ggtcaggatc agcaactgac actcacggtt ggggaaaacg
     5881 gccactatac gctggaaggt gaagagttca ccgtcaatgg tatggtcggc cagcgtctgg
     5941 aaaaagatgg cgttgcgctg actatcgcgg acattaaggc caaaccagga acacagtttg
     6001 tcctgagcca acgtaccgaa ctggaagcga ttaacgcgtt gcaggaaacc tttaccgtta
     6061 gcgaacgcag taaagaaagc gggatgctgg aacttaccat gaccggtgat gatccccagt
     6121 tgattactcg tattctgaac agcatcgcta acaactattt gcaacagaat atcgctcgcc
     6181 aggcggcgca ggattcacaa agccttgaat tcttacagcg ccagttgcct gaagtgcgca
     6241 gcgagctgga ccaggcggaa gaaaaactca acgtttatcg ccagcagcgc gattcggttg
     6301 accttaacct ggaagccaaa gccgttcttg agcagattgt gaacgttgat aatcaactca
     6361 atgagctgac tttccgcgag gcagagatct cccagctgta taagaaagat cacccaactt
     6421 atcgtgcgct gctggaaaaa cgccagacgc tggagcaaga acgcaaacgc ctgaataagc
     6481 gggtatcggc tatgccttcc acccaacagg aagtgttgcg tttaagtcgt gacgtagaag
     6541 cgggccgtgc ggtatatctg caattactta accgccagca ggagttgagt atttcgaaat
     6601 ccagtgccat tggtaacgtg cggattatcg acccggcagt cactcagccg cagccagtga
     6661 aaccgaaaaa agcgttgaat gtggtgcttg gttttattct tggcctgttt atttcggtgg
     6721 gtgccgtgct ggcgcgtgcg atgttgcgtc gtggtgtaga agccccggaa caactggaag
     6781 agcacggcat cagcgtttat gccactatcc caatgtccga gtggctggat aaacgtaccc
     6841 gtctgcgtaa gaaaaattta ttttctaatc agcagcgcca tcgtactaaa aatatcccct
     6901 tcctggctgt ggataacccg gcggattctg ctgtggaagc cgtacgtgcg ctacgaacca
     6961 gtctgcactt cgctatgatg gagacggaga ataacattct gatgatcacc ggtgcgacgc
     7021 cagacagtgg taaaacgttt gtcagttcaa ctctggcagc ggtgatcgcc cagtccgatc
     7081 aaaaagtgtt atttattgat gccgacttac gccgcggtta ttcgcataac ctgtttacag
     7141 tgagtaatga acatggcttg tcggaatatc tggcaggtaa agatgagctc aacaaagtga
     7201 tccagcattt tggcaaagga ggctttgatg tgattactcg cggtcaggtg ccacctaacc
     7261 cgtctgaact gctgatgcgc gatcggatgc gtcaattact ggattgggcg aacgaccatt
     7321 acgatctggt gattgtcgat acgccgccga tgctggcggt gagcgatgct gcggtcgtgg
     7381 gacgctctgt cggcaccagc ctgctggttg cgcgttttgg cctgaacacc gccaaagagg
     7441 tgagcttgtc aatgcagcgt ctggaacagg ctggcgttaa tattaaaggc gcaatcctca
     7501 atggtgtgat taaacgcgcc agcaccgctt acagttacgg ctataactat tacggttata
     7561 gttactccga gaaagagtaa aactgaaggt aaagtgaccg gatgcggcgt gaacgccttt
     7621 tccggtctac gcattagaca gttcttcgtt tgtcatcagc ctcagagcat tcaggtaact
     7681 gaatgctctt ttttatgcat tacaaactgc acgccggtat gcgtgcttca ttcacgattt
     7741 gcgtaaaacc ggccaacgaa cacatgccct gcgcatttcg ctcttcacat cctgccaggg
     7801 tcagtttcac ctctccgggc ggcgtattta atgacagcgg cgttttatca cgcatctgct
     7861 gtaaagtctg gaagaccagc gaaacctgaa tccactggct gttatcgctt agccgacgcc
     7921 agcgttcaaa caccagttca ccacctggcg gcgtgttatc cggctgacct ggaagcgtcc
     7981 agttgagctc cagtgcgccg ccgagatttg ccagattagt atcgtgtccg gcaataaaca
     8041 gcactgaagt gggtaatgtc acaccatacg cctgtttttg cggtggatgg ggcgtcaacg
     8101 ctgtcatgat caaatccaac aacggggtgg cgcgactgcg ggcaacctct ggtgtgcgtt
     8161 gcagcaaata aaactgcgcg ttatgcaggc ttagcaaggt gttccactgg tgtgaatcgg
     8221 tgatccttcc ccaccccggc tccggcattc cttgtgcctg ttgcaggaga aatatctccg
     8281 tcagcattga tgcgaggctt accgcaccgg ttaatgagac attgtcggcg ctcaccttga
     8341 gttccgatgg taatgcctgc gttaatgaac agctttcgtc ctgtttctca cggttaaggc
     8401 acaattttga ttgcggaaaa ttaagcaccc gttccagttc gcgaaacgcc gtttgccgat
     8461 gcccggtaaa gtcagcaatt gaccctcctg ccctgctgag gatcgcgtca gtcacgttcg
     8521 cgttatccag ttggcaaacg ccagttttta gaggattaaa taacggatcg ggactggacg
     8581 tatctgcctg ggtatgtacg gttattgcac agtcaggtgc cagcccggcg gcgaaggctt
     8641 cgcctgtttt acgggtacgc tcgtcgacat cagcaataat cgcgacctga ccaggctgcg
     8701 ggcagccctt tttcgccagc aatccgtcag ccaccagacg ctggcgttgg taatgtccga
     8761 gataagcgat tagctcacca ccgcgcggtg tcagccaacc cagttttacc ggccaggttg
     8821 gccatgcgtc tggggtgaca tcctgcatca gttgcgtggc cttggttggg gcacgcacac
     8881 catgacgact gacaatcacc acactttcca gcttcagctc cgactcactc tgagcgaatg
     8941 cagattgcgg ggttaacgga atcaaaagag ataaaaatgg gattaagatc gctttcatcg
     9001 atatgtttcc gcttttcata tccgacatta ttgcctgatg cgacgctaat gcgccgcatc
     9061 aggcttacgt tcgttaactt ttcaatcgcg ggtccagcca taccaacaca agggtggaga
     9121 gcgaacacat caacaaaatg ccgacgaacc aaagtaaata ccacattgtc cctgctcctt
     9181 agtacagctc gttttcgtta cggcggagag tttctgtcgt catgcgtccc cacattttgt
     9241 agtagctcca gagagtgtag agcaacacaa tgggcaaaaa tatcagcacg attaccaaca
     9301 taatgctcag cgtcagctga ctggaagtgc tgtcccacaa cgtcaggctg gagattggac
     9361 tcacgcttga cggcatgaca aaggggaaca gcgtgatgcc tgccgtgaaa atcacgccaa
     9421 attgcatcaa tgacgccatt aaaaatcccc aacccgggcg accacgataa atcgccatca
     9481 ccgtcagcaa tgggcagaag aaccccagca gcgggaagat ccacaaaacg ggcgattcga
     9541 cgaaattatt catccaggca ccaggtagca ctgctaccag tttcattaac ggattggaag
     9601 gaccgttagc atcctgggcg agcagtacaa agccatcaat accgacccac agccagtaac
     9661 ccgccagcaa aaagcacagc atcaccagta gtgcggcgcg tttagtcgcc agctgtgaac
     9721 gcagatgaat cacaccaaca gttttcagtt gtaaccagac gccaccttgc aaaatcacca
     9781 tcccaaggct gagtaatccg cacaataaag ggaatggcgt cagcagttgc cagaagctgc
     9841 cgagatactc cacgcgtaat tgcggtgtga aggcaaacgg cacgccgagc agcaagttgc
     9901 cgaaggcgat accgaagact accggcggca ccagactgcc gatgaccaga ccggcatccc
     9961 acattttacg ccagcgtgca tcggcgattt ttccgcgata atcaaaggcc agcgggcgga
    10021 agaacagtga gcacagcacc aggatcatcg ccacataaaa gccggaaaac gccgctgcat
    10081 acactctggg ccaggcggca aataatgctc cacctgcgag gatcaaccag acctgattgc
    10141 cttcccagtg cgcaccaacg ctattaatca ctatccggcg ttcatcatca ttacgcgcca
    10201 ccagcggcag caaacagccg atccccatgt caaatccgtc ggagatcata aagaccacca
    10261 ggatcacgcc aatcagcagc caccagatga agcgcaatgt ttcataatca aacatgattt
    10321 tctcctttac ccctgttgct gcgtcggttg ttcactctgc atcgcgctcg gccccagacg
    10381 ggcatatttc tgcatcaggt agacttcggc gattaagaac agggtgtaaa gccctacgat
    10441 catgatcagt gagaaagcca gttgtcctgt ggttaaagcg gagtgcgcgg agtatgtcgg
    10501 taagatgtcc tgtatcgccc acggctgacg accaaactcg gtcataaacc acccggcttc
    10561 aatcgcaatc cacggcaacg gcaaactcca gagcgccatt ttcagcaccc agcgatgctg
    10621 gtcgattttg ccacgcagcg tctggacaag cgcaatcagc atcaccagta gcagcaggga
    10681 accacagccc accatgatgc ggaaactcca gaataccggc gcaacctgag gtatcgcgcc
    10741 acgcatcgcc gcctggtact gtgcggctgt gacatgattc atatccggcg catagcggga
    10801 gagcaacatg ccgtaaccaa ggtcgccttc cagctcccgg aatgcctgca acacatgcgg
    10861 ctcacgattg ccttgcgata tttcctgcat taacagccag gccatacgtc cgcgttgcaa
    10921 gcgtgggtag gtttcagcca tcaaattctt cagacccggc acgggtttat ctaatgagtg
    10981 agtggcgagg atccctagca gcgcgggaat tttgatagca aaggcgttac gctcttgatc
    11041 ctgttccggc caggcaacca catggaacgg tgcaggtgca ggttccgttt gccactcccc
    11101 ttccatcgcc gccagtttta ccggttgtac ttgcgcgact tcatacgcag aactgtctcc
    11161 gagttgcagg gtaccgataa tcgccagagt accgaagacg gaaccgatgg caaacgagcg
    11221 taatgcgaca tcgcgctccc gtccgcgcag taaataccag gcgctgatcg ccataataaa
    11281 catggccccg gtcacgtagc ccgccattac ggtgtgcaca aatttcacct ggctgaccgg
    11341 gttaaagacc aactcgctga aactggtcat ctccatacgc agggtgtcga tatcaaaatg
    11401 cgcaccggtc gggtattgca tccaaccgtt ggcattcaat atccacaacg cagagagatt
    11461 tgaaccgaac gccaccagcc acgtcaccag caggtgctgg tatttattca gccgttgcca
    11521 gccgaagaag aacagcccga caaaggtgga ttcgaggaag aaggccatta atgcttccat
    11581 cgccagtggt gcgccaaaaa tatcgcccac atagttggaa tagaatgacc agttagtacc
    11641 aaactgaaac tccatcgtca ggccggtagc cacgccaaga gcaaagttga taccgaagag
    11701 cttaccccag aagcgcgtca tatcgcggta gatcgttttg ccggtgacca cgtaaatggt
    11761 ttccatgata gccagcaaaa aaatcagccc cagggtaagg ggtacaaata aaaagtgata
    11821 cagcgcggtc agagcaaact gccagcgcga taaatcaatg acatcccaca tgcgcactcc
    11881 tgtaggcgaa gagagaaaaa taacccgata atggtagatc tccctcttta tcctgaaact
    11941 ctacgaccag aggtattcgc cgattgcgat attcataagc ctgaaagcga tacttaccgt
    12001 ctttttacgt cggtgcagct tccgccagcc actgacatac ctcgctaagc cgctgcgcag
    12061 agtcgaccaa atcttctggc gctgccagca ccacttccgg tattgggcag atttcataac
    12121 tttccagtaa cgggcctttt aaggtgtccg tacagcgtaa atgccagaca tggcgtaacc
    12181 ccgtggcgtt gatatagctc tcgccatagc caatggtacg aatctgaata tttcccggcc
    12241 cacagagacg tgagagaaaa agccgatcag cctcgctgat gggtaattgc gtcagattaa
    12301 tgctgtgcgg ctgcgcgtcg gggttacgta catgtgccag taactcatgc gccaacggta
    12361 ggccattcat caggccatca atgggcggcg gtaacagcga atctgtcggc aagagatttt
    12421 gcgttgccgc ctgccacaac gccagcggcg cgcagccagc ctccagtttg tcctccagca
    12481 acttttcgcc gcgacgtctg cgcacccgcc ataatccgca gaagatcgcc tcctgtattt
    12541 cactttcact gtcgtcaacc tgctgaatgc gcactgacac ttccccttcc ccgagaagcg
    12601 tattgataaa gtgacgatcg tcggcgttga gatttaacag atcgactgcc agcacctcgc
    12661 ccaacggcgg ctggcgttcg cttagttgat gttgtaactc gtttaacagc gcaatcacct
    12721 gcgggctgtg agcacattgc tccagggccg ccatactcgg ttcatcattc acctgacagg
    12781 tgatcggcag tggattcatg ctgaaactgt catcgttcgg ttgcgttcct ggccccagca
    12841 gatggaaaaa agtttcgctc atgaggcacg ctcctgctgc ggttcgacaa gcccgcgcat
    12901 caggtttatc agttccgccc acgggtgaat accattcagc acaccgcgat agtttccgcc
    12961 ggtaaacact aaagtggcgg gaaagcgaaa gacgccaaag cgatcgccga tggcttcgct
    13021 ctgctcaagg tcagcaatcg ccacctgcca tgtatagtcg ggaaactcgc gcagtaattc
    13081 gctaatcatt accggattat cgctgacctc tggcgtacgt ttcgggtcac tgcttaataa
    13141 caccacgccg tctggcgctt gcgtaagcca gtcgtcaaga cgggattcac tgaccggcgt
    13201 ccagccgcgc gccagcattc gttgccacaa cgcatcaaat ggcgtgtcgt tgctcatccc
    13261 tgctcctcct gtgtcatccg gtactggcgc aagcgaacgc cttcgtaatt ttccatcgac
    13321 agacagtcat aattgagaca gcgcgattca ttagccggtt gcggcacaat tccccacgca
    13381 gccagttgcg ccagcgccga ttgttccgca gcgggcagtt gctcccgcgc cagttcgctc
    13441 aggctgccgc cgtagtcgtc gagcattgcg ggttgcagac cgacgagggc aatatgtgct
    13501 ggcagatgtc cgcggatatc tgccagcgcc agcacttcgg agaaactgtt ctggtgcagg
    13561 ctcattttct tcgcgctgag ataagccgga atgcgttctc cggcataggt tcgcagcgtt
    13621 ccaggttcca gcccgtagtc aatggcatcg agaatcaaca gatggctggc gctttcgaca
    13681 taccccagca agttcagtcc ctgagtaccg ccatcgacaa tctccacata ctcgggccag
    13741 tggtaatggg catacagccg ttccgccacc cgcacgccga agccttcatc ggcccacagc
    13801 aggttgccca gccccattac caccacgcgt tgctcgctca tgaacgctcc ttgttactta
    13861 ttttgccaaa tttgtggcta cggtagccgt tgaccatagt ggagatcacc gtgtcgtcgg
    13921 acatgatgtc ttcacgcagc gccatgtaga catgaccgat cacaaatgcg ccaatcagcc
    13981 acatccccag ccgatgccag ctgtgaatgt ccattgagtt gccgcccgtc cagtagaaaa
    14041 attccaccac ataacggaac ggcgcaaaaa tagcgtactg gctgtgttcg ctgtacagcg
    14101 caaagccagt gatgatcata aagaccgaca tcaggaaata gccgaacatc gccgcctggg
    14161 cgatggggtt atggcctata tcggcactcg gacgttttgc cagaagcaga taccagcgga
    14221 tttcatacca cacgccctgc caccagcttt tacgccatac cggcacgata aacagctcgc
    14281 gggagtatcg attgccaaca aaagcccagt agatccgcat cagcaagacc acggtaaaaa
    14341 tcatcccggc gctgaagtga attaacctga tgtagcccat atagaacaga tacgtcgcct
    14401 cgccgctgac ggaaggtagc ggcttgccga taaagtaacc ggtgaccatc aacaccgcca
    14461 tgcataacac cgtcagccag tgccagatac gcactggcgc ttcaaagaca tagtggctga
    14521 caacgttgtc gcttttctgt tgcatgatga ttctccttcg ctgttaacgc acctgcacgg
    14581 agatcagctc gctaccgtcg tcgcccagca cgtgtgttga acaggcgagg cacggatcaa
    14641 agctgtgcag agtacgcagg atctccagcg gttgctcggg gatcgccatt ttggtgttca
    14701 tcagcgctgc ttcataagcg ccaatctgcc ctttgggatc gcgcgggctg gcgttccagg
    14761 tggtcggcac cacgcactgg tagagatcaa tcttgccatc gcgaatggcg gcccagtggc
    14821 ctaacgcccc gcgcggcgct tcggtaaaac cgacaccacg gcactctgtc ggccaagttg
    14881 caggttccca tttttccgtg gaagcagtgg cgagattgcc gtttttcagg ttggtcatca
    14941 gcttgttgaa gaaatactgc aacttacctg cggcccactg tgcttcgtgc gcgcggcaca
    15001 aaatgcggcc caacgttgac tggataccgg aaagcggcag gttcagcgcc gacatcatgc
    15061 gatcgaccga ctcaacggtc gcagcatcgc ctttgtgata agcgattaag gtgcgtgcca
    15121 gcggccccac ttccatcgcg ttaccgcgcc agcgcggcgc tttgatccac gagtagcgtt
    15181 cctgttcatt cagctgctga atgttggtat cgctgccttt gacatcgccg gggttgtacc
    15241 acgggtcggt aatgccatcg aatggatgac gcccgacctg atcgttggga taacgatacc
    15301 aggcgtggtc gacaaactcc tgcacctgct gcggatcaac caaatccact ggcagcacat
    15361 tgttgaagtc gccgttaatc accgcgccgc caggcatcag cagacttttc tcaccaaagt
    15421 cgttggcaat atccgggaat gcgccgtagc tgaggacaca tttatcagaa agacccgtgc
    15481 cgatttcgct ccacggtttg ttaaactgac cgatggctaa ggcgtcgggg atcatcacgt
    15541 tgttaatgaa gtctgccgtg cgggtgataa tcgactgcac caggttcagg cgttccatat
    15601 tgaccgcccc gaccgcgccg ctttcgtcaa tgttaatggc gcaaggcatc ccgccgacaa
    15661 tccagtttgg atgcgggttt ttaccgccaa agaccgcgtg gattttgaca atttcacgct
    15721 ggaaatcgag agcttctaga tagtgggcaa agcccatcag gttggcttct ggcggcagtt
    15781 tgtactgcgg gtgcccccag tagccattgc ggaagatccc caactgcccg ccttcaacaa
    15841 attttttcag gcggttttgt acgtcgaaga aatagccagg ggatgatttc ggccatgagg
    15901 agagactttg cgccagttcg gaggttttcc gcgggtcggc tttcagcgca tctaacacat
    15961 cgatccagtc catcccggcg agctgataga agtgcaccag atgatcgtgg caccagagcg
    16021 ttgccagcat aatgttgcgg atgatattgg cgttgtccgg cactttaata ccgatggcat
    16081 cttcgatggc gtaaaccgaa gccagggcgt gtacgccagt acagacgccg cagatacgtt
    16141 caacgaacgc ccacgcatcg cgcgggtcgc gcccttgcag gatgatctcc agcccgcgaa
    16201 acatggtgcc gcaggagacg gcattggtga tcacattctg atcgttaata ttcacttcgc
    16261 agcgcatgtg gccttcaatg cgcgtaatcg ggtcgaccac caggcggcgt ccggcattat
    16321 tgatggtgta tccctgagtt tcgtactgag tgctcatgcc tgtttatcct cattgcctgg
    16381 ctgatgttca gtttctgtag gttgctggtt atgacgtctg cgctggtcaa cggcgctggc
    16441 gactgcgtgc acaccaacag ccgctgccac cacgccaagc gcggttaaac cgacggtatc
    16501 ggcggtggaa tgagtaccca tttgcggaat atcgaccacg cggctgtaga acgaaccgcg
    16561 atcccagaaa ccattttccg cacaacccag gcagccgtga ccagactgga ttgggaaaga
    16621 aacgccatca ttccagcgtg tggaggaaca ggcgttatag gtggtaggcc ctttgcagcc
    16681 cattttgtac agacagtaac ctttgcgggc agcgtcatca tcccaactct ggacgaactc
    16741 tccggcgtcg aagtgggcgc ggcgatagca tttatcgtgg attcgctgac catagaacat
    16801 caatggacgg cccattctgt cgacatctgg caagcgatca aaggtcacca tgtaagtaat
    16861 gatggcgctc atcacatccg ggatcggcgg gcagccaggt actttgataa tgggtttgtc
    16921 ggtgatgact ttgtcgatag gcgttgcctg cgtcggatta ggtcgcgcgg cctgcacgca
    16981 gccccaggac gcgcaggttc cccaggcgat aatcgcgctg gctccggcag cggcacgttt
    17041 gagtttctca ataaacggtc gaccgctgct gatacagaac atcccctgct cgcccagcgg
    17101 cggattacct tctactgcga ggatatattt gccattgtat tgcgcgatga tgtcttcaaa
    17161 gacttcttcc gcctgggttc cggcggcagc catcaaagta tcgtcgtaat cgagggaaat
    17221 cagggaaagg atgacgtcct ttgccagtgg gtgagcggag cggataaaag attcggtaca
    17281 gcaggtgcat tccagaccgt ggatccatac caccggaatg cgcggtttgt tctccagcgc
    17341 ccaggcaatc tttggtgcca ttcccgcgcc taatcccagc gacgtggcag ccagactgca
    17401 atatttgaga aagctgcgtc gggtaacgcc ctgacgccgc atggcctggt aaaatgtttc
    17461 ctcgttattc atatcgcacg tctcttctcc ttgcgacacc ggcaggacac cgggcgctta
    17521 tggtcggcaa gcgacgtccc tgtagcaagg caaaccatcg cttcacgtga gatgctgaaa
    17581 acgaaagctc atccttctgc actggcgcac gtcgccagaa agtattgtta ataaagcgta
    17641 gtgaaacttt tgcacaaaac aatacaaact gtgtggattt atcttttagc gataaaaatg
    17701 gacctatttt tctttttgcc gggcggtggg gatgtttagc cagttgctaa acgagtaaaa
    17761 gagaaggaat tcatggtgat gaaatgatca atctgctgtt atttcacgct atcaatgcac
    17821 ttttggctgt ttttcaggca aacaaacaaa ttaggggttt acacgccgca tcgggatgtt
    17881 tatagtgcgc gtcattccgg aagtgtggcc gagcggttga aggcaccggt cttgaaaacc
    17941 ggcgacccga aagggttcca gagttcgaat ctctgcgctt ccgccaaata atcaaggggt
    18001 taccaaatgg tagccccttt gcttttttgg ctcttggtat aaacttggta taccaattgg
    18061 tatattttta ttcggatgag attatcggtc atgagcggaa cctcaggttc ctcgtctgat
    18121 gccgctcttg caacacgtta cgctgccgag tatttttgta aaacatggag tgctctaggc
    18181 ctgaaccagg cagaaggata caaagctatc tcagatctgt cgcatcacta ttttcgtgca
    18241 gaaggctcta gcccccccca atcttttttg tgacaccctg tttcactgaa catgaagaca
    18301 ctcaccttgt ccctgatatt cattagcatc atcctgatta tttaacttct ctatttaggg
    18361 aagcccgtct gatacaaagc ttctattcct cgttaaatat tctccattct ggcgccaagc
    18421 cctatctata gctgattgaa aacaacccta aaaacgtaat aatatcaaat agtttattcc
    18481 tacagccgat cattttgagt aaaatattga cgagaatttc acaaaaggta gatttcatgt
    18541 aaacggata
//
LOCUS       DAZFXT010000091        17669 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.091, whole genome shotgun sequence.
ACCESSION   DAZFXT010000091 DAZFXT010000000
VERSION     DAZFXT010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17669
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.091"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            18..734
                     /locus_tag="ACHKW0_003385"
     CDS             18..734
                     /locus_tag="ACHKW0_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7476164.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINNQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            764..1264
                     /locus_tag="ACHKW0_003386"
     CDS             764..1264
                     /locus_tag="ACHKW0_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CS1 type fimbrial major subunit"
                     /protein_id="HFO7476165.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1356..4040
                     /locus_tag="ACHKW0_003387"
     CDS             1356..4040
                     /locus_tag="ACHKW0_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HFO7476166.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEDSQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4037..5125
                     /locus_tag="ACHKW0_003388"
     CDS             4037..5125
                     /locus_tag="ACHKW0_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CfaE/CblD family pilus tip adhesin"
                     /protein_id="HFO7476167.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5213..6511)
                     /gene="exuT"
                     /locus_tag="ACHKW0_003389"
     CDS             complement(5213..6511)
                     /gene="exuT"
                     /locus_tag="ACHKW0_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HFO7476168.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            6994..8406
                     /gene="uxaC"
                     /locus_tag="ACHKW0_003390"
     CDS             6994..8406
                     /gene="uxaC"
                     /locus_tag="ACHKW0_003390"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HFO7476169.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8421..9908
                     /gene="uxaA"
                     /locus_tag="ACHKW0_003391"
     CDS             8421..9908
                     /gene="uxaA"
                     /locus_tag="ACHKW0_003391"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HFO7476170.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVVFANGKQTCNERNDFRELAIFKSGVTL"
     gene            9991..10542
                     /gene="ygjV"
                     /locus_tag="ACHKW0_003392"
     CDS             9991..10542
                     /gene="ygjV"
                     /locus_tag="ACHKW0_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HFO7476171.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10547..11791)
                     /gene="sstT"
                     /locus_tag="ACHKW0_003393"
     CDS             complement(10547..11791)
                     /gene="sstT"
                     /locus_tag="ACHKW0_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0015826 - threonine transport [Evidence
                     IEA];
                     GO_process: GO:0032329 - serine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HFO7476172.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(12190..13155)
                     /gene="alx"
                     /locus_tag="ACHKW0_003394"
     CDS             complement(12190..13155)
                     /gene="alx"
                     /locus_tag="ACHKW0_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HFO7476173.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGG"
     gene            complement(13438..14424)
                     /gene="ygjR"
                     /locus_tag="ACHKW0_003395"
     CDS             complement(13438..14424)
                     /gene="ygjR"
                     /locus_tag="ACHKW0_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HFO7476174.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VGAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            complement(14503..15186)
                     /gene="ygjQ"
                     /locus_tag="ACHKW0_003396"
     CDS             complement(14503..15186)
                     /gene="ygjQ"
                     /locus_tag="ACHKW0_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HFO7476175.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPERNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYSIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            complement(15263..15766)
                     /gene="ygjP"
                     /locus_tag="ACHKW0_003397"
     CDS             complement(15263..15766)
                     /gene="ygjP"
                     /locus_tag="ACHKW0_003397"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HFO7476176.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            15851..16987
                     /gene="rlmG"
                     /locus_tag="ACHKW0_003398"
     CDS             15851..16987
                     /gene="rlmG"
                     /locus_tag="ACHKW0_003398"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HFO7476177.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            17178..17261
                     /locus_tag="ACHKW0_003399"
                     /pseudo
     CDS             17178..17261
                     /locus_tag="ACHKW0_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
     gene            17258..>17669
                     /gene="higA"
                     /locus_tag="ACHKW0_003400"
     CDS             17258..>17669
                     /gene="higA"
                     /locus_tag="ACHKW0_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HFO7476178.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYAQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFI"
BASE COUNT         4683 a         4368 c         4491 g         4127 t
ORIGIN      
        1 tgtaagatca aatttatatg gtggcgaaaa ttaatctctt atcacggctt acgccgcttt
       61 tattcgtgtt cgctcctttt ttggcacagt caaatatgac tgtttatcct atggctgttt
      121 ccattaataa tcagggtgaa ggcaacgttc gcgtaatatc taaaagtaat gaagttcagt
      181 acatcaaggc gacagtattc cgtatcgata atccatcgac gcctcaggaa aatgaagttg
      241 agattaaatc cggtgatgca aatcatctgg ttgttatgcc acctaaattc gctttaccag
      301 ccggtagtag caaaaccgta cgttttgttg cgatggaacc agagcaaaaa gagaaaaatt
      361 atcgcgttaa atttgaagcg gttcccagta ttgatgacgt tgccacagat aaaaaagatc
      421 tctctatgca gttaacagtt aacttaattt gggggattgt tgttagtgtt ccacctcagc
      481 aacctattgc taagttagaa gtaaatgctg cccaaaaatt agttaatgca gggaatcaac
      541 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
      601 agaccgtaaa taaaaacatc ttccctggtc aggaaagaaa tcttgaaagc atatctggct
      661 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      721 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      781 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      841 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      901 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      961 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
     1021 cccggcgcaa ctggttcaaa gcctggatgc tagcaagatt gttccgctga cagtaacctg
     1081 gggcggtgaa gaaattaaag ctgatgcggc aaccactttt accgcaacca aaatctttgc
     1141 ttccgatgcg ttaactaacg gttctctggc taaaaacctg atgtttgctc aaaccaccaa
     1201 aggtgttctg gagacaggca tctaccgtgg tgtagtaagc atttatctgt ctcaggatat
     1261 ctaatccaga taacagaatt aacacgtaac aaagggaggg gtagccctcc cttattttaa
     1321 ctgttttagt atttgttatg gatttcagga ttgccatgga taaaaaatta ctggctcttt
     1381 tgatcctggc gagtctcagc ccggcagagg cgacattaac caaaattccc gcagggtttg
     1441 aggttattgc tcagggacag caggagtata tcgaggttta tttttcaggg aaaagtctcg
     1501 gtaaatatta tgcaatggtt aatcttgata ccgtaacatt tcttgatcca gcaagtctat
     1561 ataacaagct ggaactggat gtagacgatc agaaaatcgc gcatatagtg aaagaaaaat
     1621 tatcgcagcc gctagctcgc cacggtgaat tggcttgcgg ttatgtacgt actgactcag
     1681 ggtgtggttt tctgaatacc gatacgctgg aaataatcta taatgatgaa gaaagttcgg
     1741 caacgttgtt tattaatccg caatggaatt cagctttcga tgcgaagtca ttatatttaa
     1801 atccagacaa aaatactgtt aatgctttta tacatcagca agacatcaat gttctggcac
     1861 aggatgatta ccaatcgttg tctattcagg gaaacggtgc gctgggaata acagaaaata
     1921 gttatattgg tgcacactgg aatttcaacg gttatgatgc agatgatgtc agtgacagta
     1981 atgctgacgt cagcgatctc tattatcgtt atgatttttt acgtcgttat tatgtgcagg
     2041 cgggtcgcat ggacaaccgc acactattta atgcacaagg cgggaacttt acctttaact
     2101 ttctgccact cggtgcaatt gatgggatgc gtatcgggtc gacactgagc tatttaaacc
     2161 aggcgcaaag ccagcaagga accccagtaa tggttctgct ttcgcgcaat tctcgtgttg
     2221 acgcttatcg taatgagcaa cttttgggat cgttttatct caatagtggt tcgcaattta
     2281 ttgataccag ttcctttccg ccaggcagct acagcgtggc attaaaagtc tacgaaaata
     2341 accaactcac ccgcaccgag cttgtgccgt ttaccaaaac cggcggtctg actgacggaa
     2401 atgcgcaatg gttcttacag gcaggtaaaa ctacatcaca ggtttctgat gatgaaagtt
     2461 cagcttatca gctaggagta cgcctgccat tacatccgca atatgagctc tacgcagggc
     2521 tggcgaatgc cgatgatgtg agtgctttcg agttaggtaa taactggacg gcagatttag
     2581 gcggggtggg gaatcttgca atcagcgcca gcgtgttccg taacgatgac ggcggcaaag
     2641 gtgatatgca acaggccaac tggagtaatc cgggatggcc gacgttgggc ttttatcgga
     2701 ccaactctga cggcgatgct tgtacaaccg acagcagaga gagctataac gccttaagct
     2761 gttatgaaag tatttccgcg acggtttcac agaattttgt cggctggaat atgatgctgg
     2821 gttatacccg cacacaaaat aacactgatg atagtttgcg ttgggataaa cagcagagct
     2881 ttgaaaataa ctatcttcgc cagacaacag cgcaaagtat ctctgaaact gtacaactta
     2941 gcgcttcccg cgcttttgtg atgcgtgact ggattttgag tacgtcagtc ggtgttttcc
     3001 atcgtaatga caacggtggc gataacgacg acaacggctt gtacttatcg ttttcgttat
     3061 ctgacacgcc aacgatggat agcaataaca acagccattc aacaaatgtg tctacggatt
     3121 atcgttatag cgaacaggat ggcgatcaaa cgtcatggca gttatcgcat accttttata
     3181 acgattcatt cagccataaa gaacttggcg taaccgtcgg aggcctgaac accgatacca
     3241 taaacagcgc ggttaacggg cgttgggatg ggcaatacgg aaatgtctac gctaccgtat
     3301 ctgatagtta tgaccgtaag aatcatgatc atctttcggc ctttacgggc acttatagct
     3361 ctacgctggc tgtcagtcgc tatggcgtta atttgggagc cagtggtaca gacgatttgc
     3421 tgggtgcggt actggtggat gtgaaaggct tctctgaaca ggatgaagat agtcaggatc
     3481 tgcaactcga agcgcgggtg gcaggcagcc gaactttgca gcttggtcaa agtgacagtg
     3541 tgttgttccc ttatcctgga tttcagtctg gttttgttga ggttaacgac agtagccagg
     3601 gcaatcagca ggggacaaca aacatcatta acggtgcagg gaatcgtgaa ttaatgttgt
     3661 tgcctggcaa gctgcgctat cgcgaagtgt ctgccagctt taactacaac tatatcggtc
     3721 gtttattact gccggcagcg gtgaaaaaat tcccgattgt ggggctgaac agcgccatgt
     3781 tactggtagc tgaagatggc ggatttacac ttgaaattaa cggcagcgaa aaagagctct
     3841 atctgctttc cgggcagcaa ttccttaagt gtccactgag tgttgtaaag aaacgcgcca
     3901 gcattcgtta cagcggagat gttacttgta gtgtggtgac ttattcacaa ttaccggagt
     3961 cgattcaggt tcaggcacag ttgaaacagc ctaaattacg tggaaacgtt cagacggcgc
     4021 aaagggaggt tgcaccatga gaaatcgatt gattgcggcg atattgggct tgtttggcac
     4081 gctcactggc gttcaggcag ctcctgacgt gacaagtgaa attacgtatg atttggcatc
     4141 tggtagagcg gattattact tctggaagga tgaagcgtcg gcaggaaata atggatatat
     4201 gtggtatgaa tgttcgtatc ctgacctcca acaaacctgt acagctaatg gaaatatatc
     4261 gacagtacaa atctatttaa ctgaacaacg cagtgggatg cgttggccgg taaaactcaa
     4321 aggatttaaa acagccattg taagtagcga tgaagcgccg ccaggatgca aggggggcaa
     4381 agggcttcag acgaatctta aggattctaa tagatcttca tgtacagaag atggtcaaca
     4441 ttattatata tacgatacaa agtttcttac gctctacctt gagcagacag agatgaagaa
     4501 tttgccgatt ggtggcgtct ggaaggggaa agttaaatta cattcgaaca gcccggccca
     4561 ggactatttc gcaaatatta ccctgaatac gctcgacccc aaccatattg acgtgttctt
     4621 cccggagttc gcccacgcca cgccaagggt gcagttagac ttgcatccaa caggaagcgt
     4681 taacggcagc aactacgcgc aagatctgac catgttggac atgtgcctgt acgatggttt
     4741 taacggtaat gccatcagtt atgaaatcat gctcaaagat gaagggcgac cagctgcagg
     4801 gcgcagagac ggttacttct ctatctatcg tcagggaggg accaccaccg acgagggaga
     4861 acgcattgat taccgggtca aaatgtacaa cccggaaacc ggtgggcaaa ttgatgtgcg
     4921 caataatgaa aatatggtct ggaacagcat taacctgaaa cgtgtgcgtc cggtggtact
     4981 gcccggtatt cgctatgccg tgatgtgtgt gccgacaccc ttgactctgg cagtcgataa
     5041 attcagcgtg atggataaac aggccgggta ctacatgggc aaattgtcag taatctttac
     5101 gccttccttg ccaaccatca attaaccctg caaaccggac aaacgctttt ttctatataa
     5161 acgacgaagc cgtctgataa ggcggcttcg tcattacaac ttaagttaaa cgttaatgtt
     5221 gcggtgcggg atcgttatgt gtttcctgcg ccacctcaat cgccggtttg ttctgcaaca
     5281 cggtccagat aaccagcgca cctaacaggt caaatactgc cagaactgcg aacagcgggc
     5341 tgaagccgat ggtgtcagcc agtgcaccga caaccagcgc aaacagcgta cttgccagcc
     5401 atgcggacat cccggttaaa ccgttcgccg tcgccacttc gttacgaccg aacacgtcag
     5461 aagagagcgt aatcagcgcg ccagacagtg cctggtgggc aaaaccaccg atacacagca
     5521 gcataattgc gacatacggg ttggtgaaca gaccgatcat acccgggcca atcatcagca
     5581 cagcacccag cgttacgacc atcttacggg aaacgatcag gttcacacca aaccaacgct
     5641 ggaacagcgg cggcaggtaa ccaccgagga tacaaccgag gtcagcaaac agcatcggca
     5701 tccaggcgaa catcgcgatc tctttcaggt taaagccgta aactttaaac atgaacagcg
     5761 ggatccacgc gttaaaagta ccccaggccg gttctgccag gaaacgcggc agcgcgatac
     5821 cccagaactg acggttacgc aggatctgac caacggacat tttcttcgcc gtgctcacct
     5881 ggtgctgggc ttcctgacca ttaataatat agtcgcgttc ttcatcggtc agatgcttct
     5941 ggtcgcgtgg atgtttatag aaaatcagcc atgccatcgc ccagataaag ctcaatgcac
     6001 cggagatgat aaatgccatc tgccagctgt gcattacgat tgcccatacc accagcggcg
     6061 gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta acctaccgcg atggaacgct
     6121 ctttcgccgg gaaccattcg gagctggctt tcagacccgc cgggatcatc gctgcttccg
     6181 cggcaccgac tgcaccacga gcaatcgcca ggccacccca gctaccagcc agcgcggttg
     6241 caccacagaa cacggcccac agcacagcaa acattgcata accgattttc gtacccagca
     6301 catccagtac atagcccgct accggttgca tgaccgtata agcagcagaa taggctgcga
     6361 tgatatagga atactgttgg gtggagatgt ttaactcttc catcagagtt ggcgcagctg
     6421 ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc aagcgtcacc agtgcgatca
     6481 tataccaacg taacccttta attttacgca tgtaaaacct catcgttttg ttatatccgc
     6541 aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt agttacgacc gggtaaccga
     6601 aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc gtgctaattc ggcttccgta
     6661 ccggttaacc cgattcatct aacaaatgaa tgaatcggca atttgcattc cgtcgactta
     6721 tgatttgcga cggcagaaag ataacttgtc atacaacttt aaaaggtgag agccatcaca
     6781 aatgtgcgaa tatttgtagg gacattacct gacgacagcg aggccagtac tggcgcggcc
     6841 tgcagagaga tttaccactt tgagagtaat ttttttaact acgtttattg atctaactca
     6901 cgaaaatatc ttcggactct ggaaattggt gtgataactt tgtcagcatc gcaccataag
     6961 caagctagct cactcgttga gaggaagacg aaaatgactc cgtttatgac tgaagatttc
     7021 ctgttagata ccgaatttgc ccgccgtctg tatcacgact acgcaaaaga ccagccgatt
     7081 ttcgattacc attgccattt gccgccgcag cagattgcgg aagactatcg ttttaaaaac
     7141 ctgtatgaca tctggctgaa aggcgatcac tacaaatggc gcgctatgcg taccaacggt
     7201 gtggccgagc gtctgtgtac cggtgatgcg tctgaccgtg aaaaatttga cgcctgggcg
     7261 gcgactgttc cgcacactat cggcaacccg ttataccact ggacgcacct cgaactgcgt
     7321 cgtccgtttg gtatcactgg caaattgctt tctccgtcaa ctgccgatga aatctggaac
     7381 gaatgtaacg aactgctggc gcaggataac ttctccgcgc gcggcatcat gcagcagatg
     7441 aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt ctctggagca tcacgcagag
     7501 atcgccaaag acggctcttt caccatcaaa gtgctgccga gctggcgtcc ggacaaagcc
     7561 ttcaacatcg aacaggcgac ctttaacgac tacatggcga agctgggcga agtttccgat
     7621 accgacattc gccgctttgc tgacctgcaa actgccttga ccaaacgtct ggatcacttc
     7681 gccgctcacg gctgtaaagt gtctgaccat gcgctggacg tggtgatgtt tgctgaagcg
     7741 aacgaagcgg aactggacag cattctggcg cgccgtctgg ctggcgaacc cctgagcgag
     7801 cacgaagtgg cacagttcaa aactgcggta ctggtgttcc ttggtgccga atatgcacgt
     7861 cgcggctggg tacagcagta ccacattggc gcgctgcgta ataacaacct gcgtcagttc
     7921 aaactgctgg ggccggatgt aggctttgac tccatcaacg accgtccgat ggcggaagag
     7981 ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc tgccgaaaac cattctctac
     8041 tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga tcggtaactt ccagggcgaa
     8101 ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt ttaacgatca gaaagacggt
     8161 atggaacgtc agatgaccca actggcgcag ctcggtctgc tgagccgctt tgtcggtatg
     8221 ctgactgaca gccgtagctt cctgtcatac acccgtcacg aatacttccg ccgcattctg
     8281 tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac cggcggacat caacctgctg
     8341 ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg actacttcgc cattgaactg
     8401 aactaaggtc tggggttgat atgcaataca tcaagatcca tgcgctggat aacgtcgcgg
     8461 tcgctttagc agatttggct gaaggcacag aagtcagtgt cgataaccag actgttacgc
     8521 tgcgccagga tgttgctcgt ggacataaat ttgcgttaac ggatatcgca aaaggggcca
     8581 acgtcatcaa atatggcctg ccgattggtt atgcattggc ggatattgcg gcgggggaac
     8641 acgtccacgc ccacaatacg cgcacgaatc tgagcgatct ggatcagtat cgctatcaac
     8701 ctgattttca ggatctgcct gcgcaagcgg cagatcgtga agtgcagatc tatcgtcgcg
     8761 ctaacggcga tgtcggggtg cgtaatgagc tgtggatcct gccaaccgtg ggctgtgtca
     8821 acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga gaccaacaac gccgaaggta
     8881 ccgacggcgt gttcctcttc agccacacct acggctgctc acaactgggc gacgatcaca
     8941 tcaacacccg caccatgctg caaaacatgg tgcgccaccc gaacgcgggc gcagtgctgg
     9001 tgattggtct gggctgtgaa aacaaccagg ttgccgcatt ccgtgaaacg ttgggcgata
     9061 tcgatcctga acgcgttcat ttcatgatct gccaacagca ggatgatgag atcgaagccg
     9121 ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa cgataaacgc gagccaggca
     9181 aactcagcga actgaagttt ggtctggagt gcggtggttc tgacggtctt tctggtatta
     9241 ctgctaaccc gatgctgggg cgtttctctg actacgtgat tgctaacggc ggtactaccg
     9301 tactgaccga agtgccggag atgtttggcg cagagcagtt gctgatggac cattgccgcg
     9361 acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga cttcaaacag tactttattg
     9421 cccatgacca gccgatctat gaaaacccat cgccggggaa caaagcgggc ggtatcacca
     9481 cgctggaaga taaatccctt ggctgtaccc agaaagcggg ttccagcgtc gtggttgacg
     9541 tgctgcgtta cggcgagcgt ctgaaaacgc cggggctgaa cttgttaagt gcgccgggta
     9601 acgatgccgt agcgaccagc gccctggcgg gtgcgggctg ccatatggtg ctgttcagta
     9661 ctggtcgtgg cacgccatac ggcggctttg taccgacggt gaaaatcgct accaacagtg
     9721 aactggcggc gaagaaaaaa cactggatcg actttgacgc gggtcagctg atccacggta
     9781 aagcgatgcc gcagttgctg gaagaattta tcgacaccat cgttgtgttt gccaacggta
     9841 aacaaacctg caacgagcgt aacgacttcc gcgaactggc gatcttcaaa agcggcgtaa
     9901 cgctataaag tcgaaatgag ttgttaataa aaaacggcgt ttcataatgt gaagcgccgt
     9961 ttgcttttcc atacctgata aggatttatc atgaccgcgt attggctggc ccagggcgtg
    10021 ggtgtcatcg cctttctgat tggtatcaca acatttttca atcgtgacga acgtcgcttc
    10081 aaaaagcagc tttcggtcta tagcgccgtt attggcgtac atttttttct tctgggcacc
    10141 tatcccgctg gtgccagtgc catccttaat gccattcgta cattgattac cttacgcacg
    10201 cgcagcttat gggtaatggc gatttttatt gtgctgactg gcggaattgg cctcgcgaag
    10261 ttccatcatc ctgtcgaact attgccggtt atcggtacga ttgtcagtac ctgggcgctg
    10321 ttccgctgta aagggctcac catgcgctgc gtgatgtggt tttcaacgtg ttgctgggtg
    10381 attcacaact tctgggcggg gtcgataggc ggcacaatga ttgagggtag ttttctgctt
    10441 atgaatggct tgaatatcat tcgtttctgg cggatgcaga aaaggggaat tgatccgttt
    10501 aaagtagaga aaacccctcc cgccgtagac gaaaggggtt aaacaattaa ttacgcaggg
    10561 cgctatttgc cagacgatcg tcttccgcct ggcaagctgc cgcagtgaac agcacgtcag
    10621 ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac gccgatgata aagccgacgg
    10681 caaccacctg catggcgata tcgttcgaaa taccgaacat attacaggcc agcgggatca
    10741 gcagcagaga accccccgcc acgccggatg cgccacaggc acacagagaa gccaccacgc
    10801 tcaacagcag cgccgtgggc agatcgaccg ggatacccag cgtattaacc gcagccagcg
    10861 tcaacacggt aatggtgatt gctgcgccag ccatattgat ggtggcaccc agcggaatag
    10921 aaacggaata ggtatcgcga tccagattca gcttttcaca cagtgccata ttcaccggaa
    10981 tgttcgccgc agagctgcgg gtgaagaagg catacacgcc gctttcgcgc aggcacagca
    11041 gcaccagcgg gaacgggtta cgacgaattt tccaccacac cagcaatggg ttaaccacca
    11101 gcgccaccag taacatacag ccaaccagca cgaccagcag ttgcgcgtag ccccacagtg
    11161 tggagaaacc ggtggttgcc agggtagaag aaaccagacc aaaaatacca atcggtgcga
    11221 agcgaatgac cagtttcacc ataaaggtga cggcattcga catatcgttg accaggtttt
    11281 tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc aattgcccac accaggatcc
    11341 cgatgtagtt acctttcagc agcgcgtcga tggggttgga aaccatgctc attaccagcc
    11401 cgcgcatcac ttcgacaatg cctgacggcg gcgaaatatc acccgcgcta ctggataaat
    11461 gcagggtaga agggaaggca aagctgaaga ctactgcggc cagagcagca gagaaggtac
    11521 ccagcagata gaggaacaaa atagggcgga tattggtttt ctgcccgtgc tggtggttag
    11581 caatagatgc catcaccagc atcaacacca ggatgggggc aacggctttc agtgcgccga
    11641 cgaacaaagt acctaacaga ccaacagctt ccgccgcggg ttttgagatc catgccagaa
    11701 gaatccccag aacaaggccg accaggattt gttttaccag gctgccatga gccagacgcc
    11761 ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc ctttcattgt gttgtcgcac
    11821 atccctggtg cgttctgtcg acgcatcttt caggatgtaa caaagtgttt gcaaggtcga
    11881 gtataaggaa agttacagct tcgggaagcg aaaaatgctg gattttaaag agaggtcata
    11941 ttttgcaact taatggttac atttatttga cataaaaata aagccaacgt gcggcttcca
    12001 gactgatgac aacatcaaaa aaggactcgg tcagtcccct cgccccgttt ggggagaggg
    12061 ttagggtgag gggaacaggc cagcatcggt gcgaaaaatt accctcaccc cgaccctctc
    12121 cctggaaggg agagggagaa aacggtgctc atgttaatga cagggtacac ggcttaggca
    12181 gattaaaaat tatccacccc gctgcttatc atgccgataa ttcacccagg cgttgataat
    12241 aaacgtcatc accagaatgc caaacaccac gcccagcgag acggcgattg gaatatggta
    12301 gaagtcgaca atcagcatct tgataccgat aaacaccaga atcaccgcca ggccatattt
    12361 gagcatcgag aaacgctctg ctacgcccgc cagcaggaaa tacatcgcac gcaggccgag
    12421 gatcgcaaac aggtttgagg tcagcacaat gaacgggtca gtggtcacgg cgaagatagc
    12481 cggaatgcta tccacggcga aaatcacgtc gctcaattcc accagaatca gtaccagcat
    12541 cagcggtgtg gcgtacagca atccattctt acgcacaaag aaatgctcgt tgtcgatggt
    12601 gtcggtcatg cgcaaatgac cgcgtagcca gcgcaccagc ggcttgtctc caatgcctga
    12661 ttcatcttca tgggcgagcg ccatcttcac gccggtaaac agcaggaagg caccgaagat
    12721 atacaggatc cagtcgaact gcgaaattag ccagctgcca gtgaagatca tgatggtacg
    12781 cagaacaatc gccccgagca cgccatacac cagcacgcgg cgttgtaatg ccgccggaac
    12841 agagaaatag ctgaacaaca tcagccagac aaagacgtta tcgaccgcca gcgatttctc
    12901 aatcagataa cctgtgagaa aggccagtgc ctgtggatcg gcaacggcgc gaccttcggt
    12961 ttgcaccaga taccaccaga aagcggcgtt aaacagtaac gacagcgtca cccagaccag
    13021 cgaccaggcc gcagcctgtt tcatggtcat ggcatgtgcc ccacgacgcc cctgcaacaa
    13081 caggtcgata gccagcataa tggcgacaac aacagcgaat ccgccccata gcaacggcgt
    13141 gccgacagta ttcatagaag ttccttacac ataaaaaaac ggccaacgtc ggaagacgtc
    13201 agccgctgct ttttatgcat agacctcgcc ttccggcaag gtctcactta caacaaaaaa
    13261 ggaatacgtc tgcgtattcc tttcgggttg cccggtgacc ggatgtggtt tttcacacat
    13321 cgtaatgacg atcgaccggc aatgaagtta ctcccctttg caggtaacaa agtatgacag
    13381 accattcagc cagtcaatgg tctgttacat accttttaca ctgttttact ttggcaatta
    13441 tagttttacg ctatctgccg gaaaaatcac cccagtctgg cggcggatct cggtcagcag
    13501 tttggcggtg atgcgactga ccgccagccc cggatgatcc accagatgct catccaccag
    13561 ggtagcgaac agctctgctt cgtagagcat ggtattaata tgctgcggct gggtgagatc
    13621 ctgcatttgg ctgccacgcg gcacgaagca cactttctgg cattcagaca gtttttcaat
    13681 caccagcgat cctgcttcgc cctgaatctc gctcgccagg acagaatcac tgacttttga
    13741 gtgctgcaag gtgacgctga aatcaccgta atccatcacc accacgccgt gggcatctac
    13801 accgcttgcc agcaaactgg cggttgcctg cacgcttttc ggctcaccaa ataacgccac
    13861 cgccgacgcc aggcagtaaa agccgatatc cataattgaa ccgttagaga atgacggatt
    13921 aaaggtgttg ggattttcac cgtccaggta acgttgatag cgcgaggaat actggcaata
    13981 gttgaaaaag actttacgca atttgccgac tttcggcagc gcctggcgca gcaaatgaaa
    14041 gttcggcagg caggcggttt taaatgcctc aaacagcacc acctgatttt cccgcgcaca
    14101 ggcaatggcg gcacccactt ccgccagatt cgacgccagt ggtttctcgc aaatcacatg
    14161 aattttatgg ctaaggaaaa gttgtgtctg ggaaaaatgc agggcattcg ggctggcaat
    14221 atacaccgcg tcaatggcat cgctttccgc catcgcttcc agcgaggtaa acagatgctc
    14281 gacagaaaaa tcattggcga agtgctgagc ctgctcaagg ctgcgggaat atacggcggt
    14341 taacttgtat ttaccgctct catgggcggc ctcgacgaac tggcgagtga tccagttcgt
    14401 accaatcaca gcgaaacgta tcataacgcg tggatactcc ataaagggat tctgtcgtca
    14461 atgtagcacg cctttttcat cttcgacgtg cgcagatacg cattattctt ttgccggaca
    14521 gccatgctca ctaaacggcc caatcatgac cgatggcccg agatatttcg gctggcgatg
    14581 gaggattttg gcgtcaatca cggcgctgac acgagcgagt ttctcccgca gttgagtata
    14641 aaaaccgtgc ttcatattga ggtcaggcgc gttaaaaccg atagcgtcga tgctgtactg
    14701 ttttgccagc cagatggcgc gttggttatg aaattcttgc gagataatgg tgatatggtt
    14761 ttcaccaaaa acctttttgg cacgcactac cgaatccagc gttgagaatc cggcatagtc
    14821 gcagaagatc actttcgccg gtacgccttt agcgatcaaa gcctgctgca tacctgaggc
    14881 ttcatcgtaa tttttacgtc cgttatcgcc acttaccagt agccatttca cttttccggc
    14941 gtggtacaac gctgctgcgg tatcaatacg tcgggtaaag tagcgattac ccggcctggc
    15001 ccccagcaat aagcccacgt tacgctccgg aactgcgttg acatcgctcc aggtcagctg
    15061 cttgctggcg ttgaccataa cgcgatcggc aataaaaatg gtcgctcctg ctataagcaa
    15121 caacaaacag ccaatcttaa gagtccggcg agaaaaacaa atacggagaa gaaggcgggg
    15181 ctgcatggtc gttatcctta acgaaagggg ggaggtcgat tttaggcgct aaagaaagaa
    15241 aagataacgc ctgacatcat tttcagattt tgtcctgccc aagcgacaac tgcgttagcc
    15301 acagccgggt gtcgaactca agctggtggt actgcggctc catatggcag cacaactggt
    15361 aaaacgcttt gttatgctct ttctccttca ggtgcgccag ttcgtgcacc acgatcatac
    15421 gtaaaaacgg ttccggcgcg ttgcgaaaca cagtagcgac gcggatctct gcttttgctt
    15481 tcagcttgcc gccctgcaca cgagaaacag cggtatgtaa cccgagcgcg ttttttagca
    15541 catggatctt attgtcatac atcactttat tgatcggcgg ggcattacgc agaaactgat
    15601 ttttcagatc ctgagtatat tgccagaggg ctttatcggt ggcgtagtcg tgtgttcccg
    15661 gatagcgttt tgccagcaca tcgcccagac gctgttcgtt aatcagcgtt cgcacctggg
    15721 aaagtagctg ctcgggataa ccctgaagat aagtaagatt gttcataacc gcccacgaaa
    15781 aagagaaaaa gggtatactc acgcaccctt ttcaggggca acgtcgaaat tttatcattc
    15841 aggagggccg atgagccact tagacaacgg tttccgttca ctgacactac aacgttttcc
    15901 ggcgacggat gacgttaacc cgctacaggc gtgggaagcg gcggatgaat atttgctgca
    15961 acagttggac gacacagaaa tccgcggccc ggtgttgatc ctgaatgatg cctttggtgc
    16021 gttaagctgc gcgctggcgg aacataagcc gtacagcatt ggcgactcat acatcagtga
    16081 actggcgacg cgcgagaatt tacgcctcaa cgggattgat gagtcgagcg tgaagtttct
    16141 cgacagcacc gccgactacc cgcaacagcc gggtgtggtg ctgatcaaag tgccgaaaac
    16201 actggcattg ctggaacagc aactgcgtgc gctgcgcaaa gtggtcacgt cggatacacg
    16261 tattattgcc ggtgccaaag cccgtgacat tcacacttcc acgctggagc tgttcgagaa
    16321 agtgctcggc ccgaccacca ctacgctggc atggaaaaaa gcgcgcctga ttaattgcac
    16381 tttcaatgaa ccgccgctgg ccgatgcgcc gcagaccgtt agctggaagc tggaaggtac
    16441 tgactggact atccacaacc atgcgaatgt cttctcccgc accgggctgg atattggcgc
    16501 acgcttcttt atgcaacatc tgccagagaa tctcgaaggt gagattgtcg atctcggctg
    16561 tggtaacggc gttattggcc tgacgctgct tgataaaaac ccgcaggcga aagtggtgtt
    16621 tgtcgatgaa tcgccgatgg cggtcgcttc cagccgaatg aatgttgaaa ccaacatgcc
    16681 agaggcgttg gatcgctgcg agtttatgat caacaacgcg ctctccggag tggagccttt
    16741 ccgctttaat gctgtgctct gcaacccgcc gtttcatcag caacatgcgc tgaccgataa
    16801 cgtcgcctgg gagatgttcc accacgcccg ccgctgcctg aaaatcaacg gcgaactgta
    16861 tatcgttgcc aaccgtcacc tggattactt ccataaactg aagaagattt tcggcaactg
    16921 caccaccatc gccacgaata ataaatttgt ggtgctgaaa acagtgaagc tggggcgtcg
    16981 tcggtaagat tgggagttac ctgccggatg cggcgttaac gccttatccg gcctacgttc
    17041 agcacgttac tcccaggccg gataagacgc ggcaagcgtc gcatccggca atgtctgccc
    17101 agttctggaa tcgcgcttcc ataaaacaaa aaatccctgt caccatcctc tacattctct
    17161 tgtttagcgt tgttcaacgt ttatgctaca tacgtgaagt tatgacgcat aaagaatacg
    17221 atttctttac cgctgttcat cgtactaagg ggaaaaaatg attgcgattg ccgacatctt
    17281 gcaagcagga gaaaagctaa ctgcggtggc accttttctg gcgggtattc agaacgagga
    17341 acaatacgcc caggcgctgg aactggtaga tcatctgctg ctcaacgatc ctgaaaaccc
    17401 cttgctggat ctggtgtgtg ccaaaataac cgcgtgggaa gaatcagcgc ccgaatttgc
    17461 ggaatttaat gccatggctc aagccatgcc tggcggtata gccgtgattc gtacccttat
    17521 ggatcaatat ggtttaaccc tttccgatct gccggaaatt ggcagtaaat ctatggtgtc
    17581 acgcgttttg agcgggaaga ggaaattaac gctggaacac gctaaaaaat tggcaacgcg
    17641 attcggcatt tctcccgcct tgtttattg
//
LOCUS       DAZFXT010000092        17635 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.092, whole genome shotgun sequence.
ACCESSION   DAZFXT010000092 DAZFXT010000000
VERSION     DAZFXT010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.092"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            198..452
                     /gene="bssS"
                     /locus_tag="ACHKW0_003401"
     CDS             198..452
                     /gene="bssS"
                     /locus_tag="ACHKW0_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HFO7476179.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            567..1685
                     /gene="solA"
                     /locus_tag="ACHKW0_003402"
     CDS             567..1685
                     /gene="solA"
                     /locus_tag="ACHKW0_003402"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HFO7476180.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1733..1846
                     /locus_tag="ACHKW0_003403"
     CDS             1733..1846
                     /locus_tag="ACHKW0_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HFO7476181.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            2107..2673
                     /gene="yceJ"
                     /locus_tag="ACHKW0_003404"
     CDS             2107..2673
                     /gene="yceJ"
                     /locus_tag="ACHKW0_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO7476182.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            2677..3252
                     /gene="yceI"
                     /locus_tag="ACHKW0_003405"
     CDS             2677..3252
                     /gene="yceI"
                     /locus_tag="ACHKW0_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HFO7476183.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(3294..4346)
                     /gene="trhO"
                     /locus_tag="ACHKW0_003406"
     CDS             complement(3294..4346)
                     /gene="trhO"
                     /locus_tag="ACHKW0_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HFO7476184.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            4571..5491
                     /gene="lpxL"
                     /locus_tag="ACHKW0_003407"
     CDS             4571..5491
                     /gene="lpxL"
                     /locus_tag="ACHKW0_003407"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HFO7476185.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            5663..6889
                     /gene="mdtG"
                     /locus_tag="ACHKW0_003408"
     CDS             5663..6889
                     /gene="mdtG"
                     /locus_tag="ACHKW0_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HFO7476186.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            6972..7346
                     /gene="msyB"
                     /locus_tag="ACHKW0_003409"
     CDS             6972..7346
                     /gene="msyB"
                     /locus_tag="ACHKW0_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7476187.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(7347..7574)
                     /gene="yceK"
                     /locus_tag="ACHKW0_003410"
     CDS             complement(7347..7574)
                     /gene="yceK"
                     /locus_tag="ACHKW0_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HFO7476188.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(7747..10290)
                     /gene="mdoH"
                     /locus_tag="ACHKW0_003411"
     CDS             complement(7747..10290)
                     /gene="mdoH"
                     /locus_tag="ACHKW0_003411"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HFO7476189.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(10283..11818)
                     /gene="mdoG"
                     /locus_tag="ACHKW0_003412"
     CDS             complement(10283..11818)
                     /gene="mdoG"
                     /locus_tag="ACHKW0_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HFO7476190.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            12212..13369
                     /gene="mdoC"
                     /locus_tag="ACHKW0_003413"
     CDS             12212..13369
                     /gene="mdoC"
                     /locus_tag="ACHKW0_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HFO7476191.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(13377..14858)
                     /gene="clsC"
                     /locus_tag="ACHKW0_003414"
     CDS             complement(13377..14858)
                     /gene="clsC"
                     /locus_tag="ACHKW0_003414"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HFO7476192.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(14800..15333)
                     /gene="ymdB"
                     /locus_tag="ACHKW0_003415"
     CDS             complement(14800..15333)
                     /gene="ymdB"
                     /locus_tag="ACHKW0_003415"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HFO7476193.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(15428..15739)
                     /gene="ymdA"
                     /locus_tag="ACHKW0_003416"
     CDS             complement(15428..15739)
                     /gene="ymdA"
                     /locus_tag="ACHKW0_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmdA"
                     /protein_id="HFO7476194.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(15860..16192)
                     /gene="csgC"
                     /locus_tag="ACHKW0_003417"
     CDS             complement(15860..16192)
                     /gene="csgC"
                     /locus_tag="ACHKW0_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly chaperone CsgC"
                     /protein_id="HFO7476195.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(16251..16706)
                     /gene="csgA"
                     /locus_tag="ACHKW0_003418"
     CDS             complement(16251..16706)
                     /gene="csgA"
                     /locus_tag="ACHKW0_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli major subunit CsgA"
                     /protein_id="HFO7476196.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(16747..17202)
                     /gene="csgB"
                     /locus_tag="ACHKW0_003419"
     CDS             complement(16747..17202)
                     /gene="csgB"
                     /locus_tag="ACHKW0_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli minor subunit CsgB"
                     /protein_id="HFO7476197.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         4427 a         4468 c         4170 g         4570 t
ORIGIN      
        1 attgcacaat atgctaaaaa aatatgaatg acatgctgtt ttattgttca tataaatctt
       61 aaaagcagaa atttatctgt tccccccttt aaaaattcgg tgaataccct tacttattgg
      121 tatactgaag ttgcttcaga taaaaacgca ttgaacctcg aataacgttg tctagtaaca
      181 cgaattaggg ggccatgatg gaaaaaaata atgaagtcat tcagactcat ccgctcgtag
      241 ggtgggacat cagcaccgtt gatagctatg atgcgctgat gttgcgtttg cactaccaga
      301 ccccaaataa gtccgagcag gaagggactg aagttggtca gacgctctgg ttaaccactg
      361 atgttgccag acaatttatt tcgatattag aagcaggaat cgccaaaatt gaatccggtg
      421 atttcccggt aaacgagtat cggcgtcatt aacatgacat gaccatccac ccaaaaggca
      481 cctcaccggt gcctttacca tttctgggtt gtatatctgc attcagttaa ttactctgct
      541 taaaccatga gagcagagag aaaaaaatga aatacgatct catcattatt ggcagcggtt
      601 ccgtaggcgc tgccgccggg tattatgcaa cccgcgccgg tttaaacgtg ctaatgaccg
      661 acgcccatat gccaccgcat caacacggca gccaccatgg cgatacgcga ttaattcgcc
      721 atgcttatgg tgaaggcgaa aagtatgtcc cgctggtcct ccgcgcgcaa acgctgtggg
      781 atgaactttc ccgccacaac gaagatgatc ccatttttgt acgctctggt gtcattaacc
      841 tcggcccggc tgactccgca tttctcgcca acgtcgccca cagcgccgaa cagtggcaac
      901 tcaacgttga aaagctcgat gcgcaaggga ttatggcccg ctggccagaa atacgcgtcc
      961 cggacaacta catcggctta tttgagactg attccggttt tttgcgcagc gaactggcga
     1021 ttaaaacctg gatccaactg gcgaaggaag cgggctgtgc gcaactgttc aactgcccgg
     1081 tcaccgcaat tcgtcatgac gatgatggcg taactattga aaccgttgac ggtgagtatc
     1141 aggcgaaaaa agcgattgtc tgcgcgggaa catgggtaaa agacctgctc ccggagctgc
     1201 ctgtccagcc cgtacgtaaa gtatttgcct ggtatcaggc cgatggccgc tatagcgtga
     1261 agaataaatt cccggcgttt accggtgaac tgcccaatgg cgatcaatat tatggttttc
     1321 cggcagaaaa cgacgcgttg aagattggca aacataacgg aggccaggtt atccattcag
     1381 cggatgaacg tgttccgttt gcggaagtgg tcagcgatgg ttcggaagcc ttcccgttct
     1441 tgcgcaatgt attgccgggt atcggttgct gcctgtacgg cgctgcctgc acctatgata
     1501 attcgcctga cgaagatttt attatcgata ccctacccgg ccacgataat acactgctca
     1561 ttaccggcct gagtgggcac ggttttaaat ttgcgtcagt tttaggggaa atagctgccg
     1621 attttgcgca agacaaaaaa agcgattttg atttgacgcc attcaggctt tcccgcttcc
     1681 aataatcatc attacggcct ccagaatgag gccgttttta caggagtatt taatgcgtcg
     1741 gctgttgcac tatctcatca ataatattcg cgagcatctg atgctttatc tttttctctg
     1801 gggattgttg gcaattatgg atctgatcta tgtgttttat ttttagtttt ccgcactgac
     1861 ataaactgac aaatatttaa tctccctttc atttagcgtg aaaaattgaa tgagattatt
     1921 cctgcttcta cagcactcac cagacatctc tgatgctgaa cccactaaac ttatgaagat
     1981 tctttcatta ttaattcaaa attattgaaa taaaaataaa tatcattcaa tttgttattt
     2041 ttgtgttgca attcattctc cgacacgcca ttttctttct ctcgcaaatt cgcattggat
     2101 gcacttatgt cattcacaaa tacccctgaa cgctatggtg ttatatctgc ggccttccat
     2161 tggctaagcg caattattgt ctacggcatg tttgctttag gtctgtggat ggtcacgctc
     2221 agttattacg atggctggta tcacaaagca cccgaactgc ataaaagcat cggtattttg
     2281 ttaatgatgg ggctggttat tcgcgttctg tggcgtgtca tatctccacc gccgggtccg
     2341 ctgccgagtt attcgcccat gactcgtctt gccgcaaagg ctggacatct cgcattgtac
     2401 ctattgctgt tcgccatcgg catcagcggc tatctgatct caactgccga tggtaaaccg
     2461 atcagcgttt ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca
     2521 gactttgccg gtgccctgca tttttggctc gcgtggagcg tcgtagtact gtccgttatg
     2581 cacggattta tggccctgaa gcatcatttc atcgataaag acgacactct gaagcgcatg
     2641 ctgggaaagt cgtcatctga ctatggagta taaaaaatga aaaaaagcct gcttggttta
     2701 accttcgcgt ccctgatgtt ctctgccggt tcagcggttg ccgccgatta caaaattgac
     2761 aaagaaggtc agcacgcctt tgttaatttc cgcatccagc accttggcta tagctggtta
     2821 tacggcacct ttaaagattt cgacggtact tttacctttg acgaaaaaaa tccggctgcc
     2881 gataaagtga atgtgacaat taacaccacc agcgtcgata ctaatcacgc cgaacgcgat
     2941 aaacatcttc gcagtgcaga tttcctcaat accgcaaaat atccacaggc aacattcacc
     3001 tccaccagcg tgaagaaaga cggtgacgaa ctggatatta ccggcgatct gacgctgaat
     3061 ggcgtaacca aacccgtcac gctggaagcg aaattaattg gtcagggcga cgacccatgg
     3121 ggtggtaaac gtgcaggctt cgaggccgaa ggcaaaatta agctcaagga cttcaatatc
     3181 aagacagatt taggtccagc ttctcaggaa gtggatctga ttatttcagt ggaaggcgta
     3241 cagcagaagt aagagtgaaa tgttgccgga tggcacgcat ccggcaatga tatttattcc
     3301 gttggatcag gaatgcccag tgttgtattc agacgtccac gagacttatt aaagatctta
     3361 ttgccatttt cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct
     3421 tcgcagcaaa tctcactaca acaacctttg tatttttccg cacatactgg acactgaata
     3481 aacagcaggt ggcagccatc atttttacag ttggtatggc tgtcgcacgg cgcgccgcac
     3541 tggtggcaat gcgcgataat ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca
     3601 aaatttttgc caataaaacg caccggcaag ccctgctcgc gcgccttacg ggcgtattca
     3661 ataattccgc cctcgatatg ccagactttg ttgaatccgt tatgtttcat ccaggcactg
     3721 gccttttcac aacgaatgcc gccggtgcag tacatgacga tttttttatc tttatgtgcc
     3781 tgcatcatct cgactgcttt tggcagctgc tcacggaagg tatctgccgg aatttcgagc
     3841 gcgttttcaa agtgccccac ttcatattca tagtggttac gcatgtcgat aaatagtgca
     3901 tcgggatcgt caagcatggc gttcacttcc gccgcttgca gatactcacc aacattgctg
     3961 gcatcaaagt gaggatcgtc aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc
     4021 agtacccaga aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa
     4081 gccgggtcga aggcatagag ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg
     4141 atttgcgcgt taatgccctc atgcgccaga tacactcgcc caaaaacatt cagcgcggta
     4201 aacagctgat ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa
     4261 aacgaaatgg tggttcgcgg ttcgctctca gccaacattt tggcttttag cgcgtcgttg
     4321 gaaatgcggt tgtgtaacac tggcatggtg tacggttcct gcgagatggg aaagtaaaaa
     4381 tccgcggcat gatatagcaa ttatcgataa ttaacatcca cacattttac gctacatttg
     4441 cgcattaaaa attatttgtt atttacaagc gcggcaattt cgcccagtct tcagccacaa
     4501 attttggttg cgggcgaaaa aatgcgacaa tacatacaat tgcccgaata ggttgaaaaa
     4561 caggattgat atgacgaatc tacccaagtt ctccactgca ctgcttcatc cgcgttattg
     4621 gttaacctgg ttgggtattg gcgtactttg gttagtcgtg caattgccct acccggttat
     4681 ctaccgcctc ggttgtggat taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa
     4741 aattgtgcat cgcaacctgg aactgtgctt cccggaaatg agcgaacaag aacgccgtaa
     4801 aatggtggtg aagaatttcg aatccgttgg catgggcctg atggaaaccg gcatggcgtg
     4861 gttctggccg gaccgccgaa tcgcccgctg gacggaagtg atcggcatgg aacacattcg
     4921 tgacgtgcag gcgcaaaaac gcggcatcct gttagttggc atccattttc tgacactgga
     4981 gctgggtgcg cggcagtttg gtatgcagga accggggatt ggcgtttacc gcccgaacga
     5041 taatccactg attgactggc tacaaacctg gggccgtttg cgctcaaata aatcgatgct
     5101 cgaccgcaaa gatttaaaag gcatgattaa agccctgaaa aaaggcgaag tggtctggta
     5161 cgcaccggat catgattacg gcccgcgctc aagcgttttc gtcccgttat ttgccgttga
     5221 gcaggctgcg accacgaccg gaacctggat gctggcacgg atgtccggcg catgtctggt
     5281 gcccttcgtt ccacgccgta agccagatgg caaagggtat caattgatta tgctgccgcc
     5341 agagtgttct ccgccactgg atgatgccga aactaccgcc gcgtggatga acaaagtggt
     5401 cgaaaaatgc atcatgatgg caccagagca gtatatgtgg ttacaccgtc gctttaaaac
     5461 acgcccggaa ggcgttcctt cacgctatta aatctcccct gccggatgct tcagaatggc
     5521 atccggcatt accacagcaa atccccctga tttagcgata aaagctctct ggattgcgcc
     5581 ccctggaagt cgggcgcata attagtgtgc ttatcttttc ttcttatgtt caccgcgcct
     5641 ggcgcaccaa cagcggattg ctatgtcacc ctgtgaaaat gacaccccta taaactggaa
     5701 acgaaacctg atcgtcgcct ggctaggctg ttttcttacc ggtgccgcct tcagtctggt
     5761 aatgcccttc ttacccctct acgttgagca gcttggcgtt accggtcact ccgccctgaa
     5821 tatgtggtcc ggtattgtct tcagcattac atttttattt tcggccatcg cctcaccgtt
     5881 ttggggtgga ctcgccgacc gtaaaggccg aaaactcatg ctattacgct ctgccctcgg
     5941 catgggcatc gtgatggtgt tgatgggact ggcacaaaat atctggcagt ttttgatcct
     6001 acgggcgctt cttgggttac ttggcggatt tgtccccaac gctaatgctc ttatcgccac
     6061 acaagtaccg cgtaataaaa gcggctgggc gctgggtacg ctctccacag gcggcgttag
     6121 tggtgcgttg ctcggcccaa tggctggcgg cctgctcgcc gatagctacg gcttacgtcc
     6181 ggtattcttt attaccgcca gtgtgctcat actctgcttt ttcgtcaccc tgttctgcat
     6241 cagagaaaaa ttccagccgg tcagcaaaaa agagatgctg cacatgcggg aagtggtgac
     6301 atcacttaaa aacccgaaac tggtactcag cctgtttgtc accacgttaa tcatccaggt
     6361 ggcgacaggc tcaattgccc ccattctgac gctgtatgtc cgcgaactgg cgggtaacgt
     6421 cagtaacgtc gcctttatca gtggcatgat cgcctcggtg ccaggcgtgg cggctctgct
     6481 aagcgcacca cgactcggca aacttggcga tcgaatcgga cccgaaaaga tcctgattac
     6541 agcgctgatc ttttctgtac tgctgttgat cccaatgtct tacgttcaga cgccattgca
     6601 acttgggatt ttacgttttt tgctcggtgc cgccgatggt gcactactcc ccgccgtaca
     6661 gacactgttg gtttacaact cgagcaacca gatcgccggg cgtatcttca gctataacca
     6721 atcgtttcgt gatattggca acgttaccgg accattgatg ggagcagcga tttcagcgaa
     6781 ctacggtttc agagcggtat ttctcgtcac cgctggcgta gtgttattca acgcagtcta
     6841 ttcatggaac agtctacgtc gtcgtcgaat accccaggta tcgaactgat ttttcgcctt
     6901 tcatacttgc aaaagcggag aatcagctat ccttttccct gaaacctcat caactcaaag
     6961 ggagaatcgt gatgaccatg tacgcaacgc ttgaagaagc cattgacgct gcacgcgaag
     7021 aatttcttgc agacaacccc ggcatcgacg ccgaagatgc gaatgtgcaa cagttcaatg
     7081 cccaaaaata cgttttgcag gacggcgaca tcatgtggca agttgagttt tttgccgacg
     7141 aaggggaaga aggtgaatgt ttacctatgc ttagcggtga agccgcgcaa agtgtttttg
     7201 atggcgacta tgatgagata gagatacgcc aggagtggca ggaagagaat acattacatg
     7261 aatgggacga gggggaattt cagcttgagc caccgctgga taccgaggaa ggacgcgcag
     7321 cagctgatga gtgggatgaa cgttaatcac tcatacgggc catgatgaat gtcgatcggc
     7381 agcagtaaag tatcaaaaac cagagagaat ggcagatcaa ggatcgtgac ataacgccag
     7441 gcggagtcac gcacatccca ttgcacaccg ggataatatt ggttgccatg cccctgcccc
     7501 ggtatagtgc gactaataat gctgccacag ccactcagca aggtcaccat gatgctcacc
     7561 acaattaaac gcatttgata cctctttcgt aaaaaaatac cggcataacg ccggtatttt
     7621 tattggctcg ttgtttttgc cggatgcggc gtgaacgcct tatccgaccc acaaaatctt
     7681 gcaaaatcaa taaattgcag gaacgatgta ggcctgataa gcgtagcgca tcaggcaact
     7741 acgtttttat tgcgaagccg catccggttt acgcaatgcc agtggattga gctttatccc
     7801 ttcgtaataa ctcacccatg aagaatatct ctccggggaa ttccagacgc ggaaatgcag
     7861 acgggccatc gtcaccggat cgcttagcag caccaggcga cgatcgcgat tcagcttctc
     7921 tggcgtctcg ttcagcgcct gttcaacgtg gcggtcacgg gcgatttcca gcaccttact
     7981 ggcgcggtga cgcgcggtcg ccattgcggt tgccagagcg ttaaatgacg ggttaaacac
     8041 tgcgtgcata aagccatcat caagggagcg ttgacgattc atctcaagga accgatcggt
     8101 atcaaccagc acctgcggcg gcgaatactc ttccgggatc aggaacagtt tccagcgttt
     8161 ggtgcgcaga ccaacggtgg cacggctgga aatcaccgaa acaaacggtg acaggatcaa
     8221 cgagaagaca atcggtgcca gccagaacag gaaacgcaga tccaaccacg ccataccaac
     8281 agcccacact aaccctaaca gcagctgtga gccgtggcgt ttgaacgctt caccccagga
     8341 agtggagtca tcatcacgct gcggtgaatt ccacaccact tcccagccaa ggaacgcgct
     8401 gacgacgaag accgtatgga acagcatgcg taccggagcc agcagcacgg aaaacagcac
     8461 ttccagcagc aacgataatg taacgcgcca gaagccgccg tattctttcg ttcctttgca
     8521 ccagataagc aaaatgctca ataacttcgg caggaacaac agcaccatgg tcgaagcaaa
     8581 aagtgcaatc gccagctcag gacgccactg cggccacacc gggaacaact gccgtggttg
     8641 caggaagtat tgcggttcgg tcaacgcatg tactacctgc aatgcagtag agagcgcgag
     8701 gaacataaac cacagcggag cggagagata agacatcacg cccgtcagga ataccgcacg
     8761 gtgaaccggg tgcataccct tcaccaggaa cagacggaag ttcatcaggt taccgtggca
     8821 ccagcggcgg tcacgtttta gctcatcaag caagttaggc ggcaattctt cataagaacc
     8881 cgggagatcg taagcaatcc agacccccca acctgcacgg cgcatcaacg ccgcttccac
     8941 gaagtcatgt gacaggattg aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc
     9001 gcagtgctcg ataaacggtt tcacgcggat aatcgcgtta tgtccccagt agtgcgactc
     9061 gccaagttgc cagaagtgca aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa
     9121 ctgctgacag cgcgcataca gcgtatccat accggacgct ttcggcgacg actgaatgat
     9181 cccggcgttc gggttggctt ccatcaggcg caccaggccg cataaacaat caccggtcat
     9241 taccgagtca gcatccagca ccaccatgta gctgtactgg ctgccccagc gacggcagaa
     9301 gtcatcgata ttaccgcttt tacgcttcac gcggcgacgg cggcggcgat agaaaatctg
     9361 accttcgcca ccgacttcag cgataagctc catccaggct ttttgctctg cgacgcagat
     9421 atccgggtta taactgtcac taagaatgta gacatcaaag tgtttggcat tcccggtggc
     9481 ttttactgat tcccacgttg cacgcaggcc agcaaaaaca cggttcacgt cttcgttaca
     9541 gataggcatg atcaacgccg tgcgatgctc cgggtttaat ggttcatcgc caactgttga
     9601 cgcagatata ctgtatttat cgcgaccaat aagcagttgc aggaagccca ttaacgccgt
     9661 ccagaatccg gcggacaccc aacagaacag taccgcaaag aggatcagga taccggtttg
     9721 cagcatataa ggcagaagct gcataaagga aacccacaaa tcctgaccaa ccatatccat
     9781 aggattaatc agcgcccaac cctgataagg aagaatggtc ttcatatacc aggtcgcgac
     9841 gacagtttgc gcgagcgtca ggatcaacag aatgtaacgg cggatggtac cgacggtacg
     9901 ccacttttgc tcgctctcct gctcttcttt ggtcaaacga gccagatagc gcggcgtgac
     9961 atcgcgtcca cgcaggcgat cccagaaacg gcctaccggg ttggtacgcc acgggtcggg
    10021 aaacatcgag gagcgttttg cttctggcat cgccttcagc tgatcgcgcc cttcgtcgtc
    10081 tttaattaac tgtccatcag caagtgaatc tggccaggct tgttccagac gcgcctttac
    10141 cgagccttgc ggggaatcat cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg
    10201 atgaacggcg cggatatcag tcttcggcaa tgccgctttc tcgcttgcgg cgatgggcat
    10261 tgcgtcaatg tactcagttg tcttattcat tggcaggtaa ctggtagctc caggtttcac
    10321 tcaacgtctg atcggcattc accagcgcag cacgcatttc agtggttttc ttggcatctt
    10381 tcactttcac acgcatcacc agacgccagc ctttggtaac cgggttatag cgcaccgtgc
    10441 tttcaactat ctcaccatta tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg
    10501 gcagtttttt catctctgcg ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct
    10561 ggcgaatcag gttcgactgc ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg
    10621 cgttatctgg cgcatgcagt ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt
    10681 tcatctcttt acccggctcc ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt
    10741 tggtttcatc gttggttgga atttctacca gctcaacgct gcctttaccc cactcccctt
    10801 tcggagtcac ccatgcgctt ggacgaagat cgtaacgatc atcgagatct tcaaagcggg
    10861 agaaatcacg accgcgctgc aacagaccga agccttgcgg gttttccata gagaagctgc
    10921 tgaccgctaa atgtttcggg ttattcaacg gacgccagat ccattcgcca ttaccggcat
    10981 ggatagagag accgttagag tcgtgcaact ccggacgata gttatttgca ggcgacggtt
    11041 ggttcggccc aaacaggaac atactggtta acggtgcaac ccccagtttg ccgactttat
    11101 cgcgcagata gattttcgac tgcacatcca caaccgtgtc acgccctggc ataactacga
    11161 atttgtaagc acctgtcgcg cgcggcgagt caagcaatgc ataaatggtt aaacgtttat
    11221 cagtcggttt tggacgctcg atccagaact ctttgaagcg tggaaattct tcacccgatg
    11281 gcaaggcggt atcaattgcc aggccgcggg cagaaaggcc ataaacctga cctgcaccaa
    11341 tcacgcggaa atagctggcc ccgagcatgc tgacgatttc atcgttttta tctttgctgt
    11401 tgatcgggta aagcacttta aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat
    11461 gctgaacatc gccgaaagtg aaataatccg ggctgtattt gattcgtttg actgcggtgg
    11521 cagtcacttc atttattttg accggggtat cgaagtacat accctgatgg tagaactcga
    11581 gtttgaatgg ggtcttcaga ttgttccagt acgctttgtc atgattaaac tggatctgct
    11641 gatagtccgc gtatttcata tcgcggaaaa cggagggcaa gttgcttttg ggcgcctcat
    11701 agcctttccc ggctaaggat tgagcttgct ttgcgacatc atcaatactg aaagcccagc
    11761 ttgaagatgt atacagggtt aacattactg cagcactcaa ccaacgcatt ttcatcattt
    11821 gtagtttatg tttcataatt agtaagcact tccccctttg tgtgcttata tcgatccgat
    11881 ctattttaat ggataaccag ccattccgac aacagaatcc cggtatcgtt cagcacgctg
    11941 gctcatggat taagcacctg attgcaaccg ttttcacttt ccgtgcttct cctaaagaca
    12001 gaagcatcga cttcgtgtag ggtttgccag caattttatt tatcaaccgt cttacagacg
    12061 cttccattct ggttagtggt agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct
    12121 ggcgcggtca ggtcggtgtc gctatggcaa caagcctaaa ggcataaccc gacagggata
    12181 agacgaaaaa tccgagatta gttaaccata tatgaatcca gtacccgcgc aacgtgaata
    12241 tttcctcgac tccatccgcg cctggctgat gttgttaggg atcccttttc atatttcttt
    12301 aatctattcg agccatacat ggcatgtgaa tagcgccgaa ccgtcattgt ggctgaccct
    12361 ttttaatgac ttcatccact cgttccgcat gcaggtattt ttcgttatat ccggctactt
    12421 ttcctacatg ctttttttac gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg
    12481 tgtaggtatc ccgatgttaa cagccatccc cttactgaca ttgccgcaat ttattatgct
    12541 gcaatacgtc aaagggaaag cggaaagttg gcctgggctg tcattgtatg acaaatataa
    12601 tacgttggcc tgggaattaa tatcacatct gtggttttta ctggtgttag tggtcatgac
    12661 gacgctgtgc gtatggatat ttaaacgcat cagaaataat ttagaaaatt ctgataaaac
    12721 gaataaaaaa ttctcgatgg taaaactatc ggtgattttt ttatgcctcg gcatcggtta
    12781 tgcggtaata agaagaacga tttttattgt gtatccgccc attctgagta atggcatgtt
    12841 caattttatt gtcatgcaaa cgctgtttta tttgccgttc tttatcctcg gcgcactggc
    12901 tttcattttc cctcatctta aagccttgtt taccacgccg tctcgtggct gtacccttgc
    12961 agcagcattg gcgtttgtcg cttatttact caaccagcgc tatggcagtg gcgatgcctg
    13021 gatgtacgaa accgagtcgg tgatcaccat ggtcctcggt ctgtggatgg tgaatgtggt
    13081 cttctccttt ggccaccgtt tgcttaactt ccagtcagcg cgggtgactt attttgttaa
    13141 cgcatcgctg tttatctatc tggttcacca cccgttaacg ctgtttttcg gcgcatacat
    13201 tacaccgcac atcacctcca actggcttgg ttttctctgt ggcctgatat ttgtagtagg
    13261 gattgcgata attctgtatg aaattcattt gcgcatcccg ttactgaagt ttttgttctc
    13321 tggtaaaccg gttgttaagc gtgagaacga taaagcacca gcccgttaag ccacatttac
    13381 aataaccatt ccacgggcaa tatcgacgcc agtctgacca taacccgctt ccagaaactg
    13441 gtggcgggtt cttttttgag aataatctct ttctttgcat gacgatcaac ccagttgatc
    13501 cgtccccacc tgtccagacg gagctgccag gccgcatcat actggctctg aataaagcgt
    13561 ttatcaatta actgtgccag cgtttcgctc tctatcacga agcccatttc agtattgagc
    13621 aatgttgaac gcggatcgaa attgaaagag ccgataaaca ccgttttacc atcgatgcta
    13681 aaggttttag catgcaggct ggctccggaa ttaccggtta tgccgcgatc gtgtaacgta
    13741 ctactttgtt cacgcgtcgg cttgagttca tataattcca cgccatagcg gagcaatttt
    13801 ttgcgccagc gcgcgtatcc ggcatggacg acagcaacat cgttagcggc aagagaattg
    13861 gttaggatcg caatctttac cccttttctc accatccgta agagttgcgc cacacctgcg
    13921 cgtgtcggta caaaatagga agagataata tcgatgcgtt cactgggtga gcccatgata
    13981 tcgaacaggc gctgcggtaa cagtgaatgc cgttttgcct tgccctcccc tttcgccgga
    14041 tcatcactta ataaacgtgt tttcgcccag ataagcggca acgttccatc aaccagatga
    14101 tttataaacg gactggattc cattttgcgt aagtaacgat gcgtcatagc atcgttatgc
    14161 caggaggcgg gtaactcgat gcgatccgcc atttcacctt ccgggacatc cagcacttgc
    14221 tgtaagggtg aaaccgattt gcaataccag tagcgggcga aatcatcggc aacgtcctct
    14281 accacgggcc ctattgccat aacatctaaa tccgaaaaaa gtggctcttc ccctgcccca
    14341 aaataggcat caccaatatt tcgcccccct accagcgtca ccacgccatc gacagtgaaa
    14401 cttttattgt gcatacggcg attgagacgg gaaaagtcgg tgatataacc aagcggacgc
    14461 agcaagcgaa acgagaaagg attaaaaagc cggacttcaa tgcggggatg gctgtcaagc
    14521 aagcgtaaaa tgtcgtcaag tccgggcgta ttgttgtcgt ccagcaacaa acggacgcga
    14581 acgccacgct ttgctgcggc taacagggcg gaaaacagta accgacccga catatcgtcc
    14641 tgccagatgt aatactgaac atccagcgta tgctctgcca tttcggccag acgataccgg
    14701 gcggcaaacg catccagact tttctccaga gggaaaagac cacactgacc gggatgttgc
    14761 gaacacagtg gcagcaccgc gctcgccagc cggggcaaat cattcatctc cttgttgggt
    14821 aaggagtctt tcgtagaggt gggcgttttc ttcatcataa cagacaaagt atacctgttc
    14881 gggtaaagcg tgacgggtaa taaattctga aacggtttta actgcgattt cagccgctgc
    14941 cgcacgaggg taaccataaa ccccagtact gattgcagga aaagccactg acgtatagct
    15001 gtttgccgcc accagtcgta ggctattgag ataggcatcc tgcaaaagct ggtcttcgtt
    15061 ttgttcacca ccacgccaga ctggcccgac ggtgtgcact acggctttag cgggaagagc
    15121 gcctgcaagc gtaataacgg catgccccgt agggcaatcg ccctgctgtt gcctgacttt
    15181 taaacaggca tccagcaggg ccggacccgc tgcgcgatga atggccccat cgacgccgcc
    15241 gcctcccatt aatgacggat tagccgcatt cacaatcaca tcaacggcca gtttggtaat
    15301 atcaccctgc acaacatgaa tacgcgtttt cataacgcct ccttacacct tcttgtttct
    15361 taagtgtaca gcaggctttt tggttaaatc gcatttttct gctcagcaac gtgcgttgtt
    15421 cagaaactta gttatattcg atgttcatta cagccagctt tttctgttta tcaagatact
    15481 gcacttttac tgaggctatt tgcgcgtttt taacattacc gctcattaac gctttgctgg
    15541 aataggtttt gcccggaaca gaaccattct gtgggcagtt catttctacg ggtgatgact
    15601 gggtgctgac gtcacatgcc ggttccacaa tttggccata aaaatggatc accccccctt
    15661 gcgcggtgct tcctgcgatg gcaataaaag gaaaaagcaa actgccgagc ataagagagt
    15721 ttaaaaaagg tctgaacatt tattctattt cctcaatgat tagtcatcct tgagggttgt
    15781 gttatccata ctttcaggtt ggctgattcg cagcagacca ttctctccag attcatctta
    15841 tgctcgatat ttcaacaaat taagactttt ctgaagaggg cggccattgt tgtgataaat
    15901 gaagtgactg tccatcagaa acagtaacaa ctattttcac ccgatcttcc ggggaaatat
    15961 ttaaactcaa cttcgtcaaa gcaataggtt gattagcagg caatgagagg gtcttttctt
    16021 gcttcgtctg actttgccct gaactgcctt cgcgcaggga caatatttgt actctgcaca
    16081 gacaagattg agtaagagtg acttcaggaa taatggtata cacatcccct tgctgggtcg
    16141 tattaaaggt tatctggctg gaaagtgccg caaggagtaa taacgcattc atattcttct
    16201 cccgaaaaaa aacagggctt gcgccctgtt tctgtaatac aaatgatgta ttagtactga
    16261 tgagcggtcg cgttgttacc aaagccaacc tgagtcacgt tgacggagga gttagatgca
    16321 gtctggtcaa ctgcagcacc gttgccacca ccgaactgtt taacagtcat ttcagaattt
    16381 ttgccgttcc actgatcaag agtggcgctg ttaccgaagc cacgttgggt cagatcgatt
    16441 gagctgtcat ctgagccctg acctacatct gcaccattac caccgccatg ctgggtaata
    16501 gtcaagtcag agttacgggc atcagtttgc agagcaagtg cagagttacc gccaccgtac
    16561 tggtaaatgt tcagctctga atttgggccg ctattattac cgccaccacc gtggttaccg
    16621 ccgccgccgt actgaggaac aacacctgcc agagcgctac cggagaatac gattgctgca
    16681 attgctgcta cttttaaaag tttcatgtaa aacccccatc ggattgattt aaaagtcgaa
    16741 tggaaattaa cgttgtgtca cgcgaatagc catttgcgac tgtctctgca ctacaattgc
    16801 cgttttttga gtaccatact gtgtaatatt tgctttatta ccagaacctt tctggataat
    16861 catcgcagta ttaccataag caccttgcga aatactggca tcgttggcac tgcccgcctg
    16921 atcaatatat gcaaggttat aatctcctgt ctggtcaatc tttgcccggt tgctactacc
    16981 ttcttgcgca acaaccgcca aaagttttga gcctccctgc cgtaactgag cactattatt
    17041 cgtcccagct tgaccaatta tggctgcctg attaaatgaa gacttactca attcatttac
    17101 cgcgaagtta tattctgaat tagctaaatc ataacctgct gcggctgcaa tcccaggcgc
    17161 acccagtatt gttaacatca taaataacaa tttgtttttc atgttgtcac cctggacctg
    17221 gtcgtacatt taagaaatta aatcatttca acttggttgt taacgcaacc tgtattttgt
    17281 taacgctgcg ttacgatgga aagtatgtct gcggaaatat ttttaataac tcacccgcgc
    17341 gttgtatttt tctttttttg ttcataccaa agtattaaaa cccacgtaaa tacggtgtta
    17401 tctacgcaaa aaatattttg ttttctttta aatctccgtt ttccgctatc aaaaatcacc
    17461 agacagtcat ttttcttgcc cgtcgctgat tgctgcgata tgtcttgcgc acaagccgtg
    17521 acaaacctcg tctttttgca gtaaaaaatt gtccacggag gtgcggagaa aaaacaagaa
    17581 cgttttacat gacgaaagga ctacaccgaa atatttttta tatgtattat tagta
//
LOCUS       DAZFXT010000093        17595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.093, whole genome shotgun sequence.
ACCESSION   DAZFXT010000093 DAZFXT010000000
VERSION     DAZFXT010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.093"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..185)
                     /locus_tag="ACHKW0_003420"
     CDS             complement(<1..185)
                     /locus_tag="ACHKW0_003420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476198.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGF"
     gene            complement(210..2843)
                     /gene="mngB"
                     /locus_tag="ACHKW0_003421"
     CDS             complement(210..2843)
                     /gene="mngB"
                     /locus_tag="ACHKW0_003421"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HFO7476199.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMDKLREIYPQRKFVMSRFEEVFEKIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(2861..4776)
                     /gene="mngA"
                     /locus_tag="ACHKW0_003422"
                     /pseudo
     CDS             complement(2861..4776)
                     /gene="mngA"
                     /locus_tag="ACHKW0_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
     gene            4945..5667
                     /gene="mngR"
                     /locus_tag="ACHKW0_003423"
     CDS             4945..5667
                     /gene="mngR"
                     /locus_tag="ACHKW0_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HFO7476200.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(5771..6640)
                     /gene="sucD"
                     /locus_tag="ACHKW0_003424"
     CDS             complement(5771..6640)
                     /gene="sucD"
                     /locus_tag="ACHKW0_003424"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HFO7476201.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(6640..7806)
                     /gene="sucC"
                     /locus_tag="ACHKW0_003425"
     CDS             complement(6640..7806)
                     /gene="sucC"
                     /locus_tag="ACHKW0_003425"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HFO7476202.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(7900..9117)
                     /gene="odhB"
                     /locus_tag="ACHKW0_003426"
     CDS             complement(7900..9117)
                     /gene="odhB"
                     /locus_tag="ACHKW0_003426"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HFO7476203.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQIIGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            complement(9132..11933)
                     /gene="sucA"
                     /locus_tag="ACHKW0_003427"
     CDS             complement(9132..11933)
                     /gene="sucA"
                     /locus_tag="ACHKW0_003427"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HFO7476204.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(12134..12850)
                     /gene="sdhB"
                     /locus_tag="ACHKW0_003428"
     CDS             complement(12134..12850)
                     /gene="sdhB"
                     /locus_tag="ACHKW0_003428"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HFO7476205.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(12866..14632)
                     /gene="sdhA"
                     /locus_tag="ACHKW0_003429"
     CDS             complement(12866..14632)
                     /gene="sdhA"
                     /locus_tag="ACHKW0_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HFO7476206.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(14632..14979)
                     /gene="sdhD"
                     /locus_tag="ACHKW0_003430"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(14632..14979)
                     /gene="sdhD"
                     /locus_tag="ACHKW0_003430"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HFO7476207.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(14973..15377)
                     /gene="sdhC"
                     /locus_tag="ACHKW0_003431"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(14973..15377)
                     /gene="sdhC"
                     /locus_tag="ACHKW0_003431"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0045273 - respiratory chain complex II
                     (succinate dehydrogenase) [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7476208.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFI
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            15758..15865
                     /locus_tag="ACHKW0_003432"
     CDS             15758..15865
                     /locus_tag="ACHKW0_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476209.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            16071..17354
                     /gene="gltA"
                     /locus_tag="ACHKW0_003433"
     CDS             16071..17354
                     /gene="gltA"
                     /locus_tag="ACHKW0_003433"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004108 - citrate (Si)-synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HFO7476210.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
BASE COUNT         4202 a         4769 c         4354 g         4270 t
ORIGIN      
        1 ataaagccac atatacgtgg cttgagagtg aagataaacg caaaattacc tgatgcgcta
       61 cgcttgccag tgtctacatc atctctgcaa tatattgaat ttgcctgctt ttgtagaccg
      121 aataaagcgt tcatgccgta tccggcatga acaaagcgcc ctttgtcaac aatctggccg
      181 cgcatatgtg gcttcggttt gattgctatt caggcaagcc ggtaactgaa cgtccgtgac
      241 tggccgggta aaaaaggccc cgatagattt gaaccttgat tttcctcggt ggtaatgtgt
      301 tcatccatca tcgtttctga gcaagaaatc acctcgcgac tgaaagcaac agtcgcatca
      361 caggttgctg attcagccgg attaaacagc cgtaaaatca cttcttgtcg gtcttcagct
      421 ttcttaagtg cgcttatcag gcatcccact gggggcattt tcaacaaact ataactttcc
      481 ggcacgttga atccggcttt gttgagcttc atcgcatccc atgggatttt gttgtaacac
      541 tgtactggag tcagccatgc tcgcgcctgc tgagctacac cagcggcggt tggcgtaccg
      601 gtataactca ataaacttag gcgacaagaa agcagaccac gtagttgtga gtccgggact
      661 ggcattttaa ttcccgaagg ccgcccaggc cttaaaaaca gatcttcttt gcccagtaag
      721 cccacgccac gcagcaacgt aatggcaaag gttttcttct cttcaccgat gacttcaaat
      781 tcacgtagcc cttcgctaaa gacagccatg ccgttacgcc cttcctgtaa ggctgcatag
      841 ttgagcatat tccataccgg aaccggcgct tctttccagc cttcttgctg ccagttgttc
      901 attgcactgt cgttcacggg gcgcgttagc gaaccaaact gggtatctgc cagaacacta
      961 tcggtgttaa aagatgtagg gatcaggacg cgaaggcgat gatcgtcagc ctggttatca
     1021 aggttgatat ccacatcaat acgtctgctg ttatgactca gagtgacaac catctccacc
     1081 cctactctgc ccgtggattg ccggacgctt cgttccaaca aattgagcgg caccgccatg
     1141 tcatagcgga taacagccct gctctgccag gcctcatgag taatctcgca ttgcggtttc
     1201 gctgttgcag aagtcattac ccactcttct tttgcgggtg aatagtcata ttcatcacca
     1261 tcatcagagc tttcttcaat ttgcaacacc cggtcatagc gcacaccgct atctttatct
     1321 accagttgta gcgttccatc ctcattgagc gcaatttgcc agaaagcatt ttccagtatc
     1381 ccttcagcgt cactttttgc tgcaatcacg ttgccaggct gattcgcttc gatataaagc
     1441 gtgcgatagc ccatagaagg gacaatctgg ttgatctgta tatcaaactc cataaaggga
     1501 tcgtaattac cgtaatgaac tatttgccga tcgattaggc ctggatcgat ctcacgggca
     1561 tggcgaataa aatacggtac aggctgaccg cgatcgtctc gtagattaaa ctggctggcg
     1621 cgcaggcgca cagtggtgtt aataacttct tcccgcggcc agggcatcag gttaaacagc
     1681 acgagtttgt cggcatcact ctgtggcatg ttatcggcaa ttttgcgcat gtagaaacgc
     1741 agcagattat ccgccatgtc ttcagccagt tcgaagcggg cgacaatttc gcgatgaact
     1801 ttgtcactgc aacaacaacc gatactgtcg tgggcatgat tttttaagat ctctttccac
     1861 attttctcca gcaagccgtg gtggtattca aaacccaacg tccaggccag cgttgccagc
     1921 ggttccagca gattaacaat cttattttca atacgtgcgt gggcaatttt gatatccatg
     1981 cgcgtggaac cgatggtgcg atgtacgcgc atatatttgc catcgataaa ttcacctttc
     2041 agggtggcca gactttcacg ctgcgcttcg atcttctcaa atacctcttc aaagcggctc
     2101 atcacaaact tacgttgagg gtagatctca cgtagcttat ccatcacttc gaagatattt
     2161 tgctgcaatg gcatctggtc atgcccattc ggcagcaaaa tctctttggt tacagacgct
     2221 ttttccagca cgtcaaaata actgtcgagg cgtttacgta atccgttttc gtcggcaggt
     2281 aagtacttac cgatggcgta gcccagcggc agcacctgcg ccgtcacttc gctaccgtca
     2341 ctgctttgcc acaaaaactc ggttttatca gtaccgtggc gctccgaaca tccgcgccag
     2401 aacatggtgc gggtaatgcc aaatccattg tagatatgcg gcagttgccc ggacatgcca
     2461 aaggaatctg gtaaataacc tattttcatc ggctcgccaa acgcgaggca gtcacgcatt
     2521 ccgtacatca gattacggac gatggattcc gcagaaacaa tcgtggtatc ggtctgggta
     2581 taccaggggc cgataatcaa cttgccggct tctacctgtt tcttcacgcg gtctttgttt
     2641 tccggtttca ccgcgaaata atcttcgagg atcgccgttt gcccgtcgag tacgtaatat
     2701 ttgtattcgt tgtcctgttc cagtcggcac aggatctctt ccatattatt gaccagcaga
     2761 atgcgtgact cttcggtggt gaaataccac tcccgatccc aatgcatatg cggggtgatg
     2821 tgaacgcgag ataccgcttt catcgtcgtt tcctttttgt ttatggcatt acgccatcag
     2881 tcagatagtt gccatgctta accgcgtgac gccgccagat caggagaatt gcagtcgaga
     2941 ttgcagcccc caccaatgcc gcaccaaacc agccaattgc cgccataaca ccgcccgcgc
     3001 cattatcatg aagtaaaaag agcgagaaaa tgccggcacc gggtgtcgaa aggccgatat
     3061 tcatcgcacc gacaatagcg cccgttacca tagagcccag cacaaacgaa ccaataaccc
     3121 gcagcggatc ttcaatcgcc atcgggatcg ccccttcggt aatacctgcc agccctaaca
     3181 gccaggtgga tttcccggtc tcaatttcaa actctttaaa caggcgcggt gcgagcatcg
     3241 tggaagcggt tacggtaaat gccgaaacca ttttgacgga ggcgaaaatg gcatacgggc
     3301 cgtaaacgcc gttcgccatt gcgcccaggc agaatgcata agcggctttg ttcactggcc
     3361 ctccaaggtc aaaggaacac ataaaaccga gaatggcacc cagcaacagc gcgttacttc
     3421 ctgacagacc gttcagccag gcggtaagcg agttattgat ccaggcgaca ggttctccca
     3481 ccacaaacag catcagactg cccgctccca acgtaccgag caccgggtag agataaaaag
     3541 tcaggaatcc attgaattta ctgctaagac gcaagtgatt tttcacccag cgcatcaagt
     3601 aaccggctat caatccgcca acgaccgcgc cgagaaaccc ggagccgatc atgttggcgg
     3661 caagtccagc cgcaaagcct ggcgctaacg ccggtttatc tgccagagaa taggcggtat
     3721 aggccgcgag caccggtacc atcaaaattc cgagcagccc gccgcccagc ttgcggtaca
     3781 tccacagcca ggagttttct tcattaaaca gatcttgtag cccgaagatt tgcgacagta
     3841 ataccgcgac cgccagcacc gtgccccccg cgacaatcaa cggtacggca aaagagattc
     3901 cgctcaacag tgcctgtttc agctccgttt tgacactttt caccggttgc gtatcttgct
     3961 gtacggtacg cgtctcatcg ctacgcttga gggtaagcgc ttgttggatc aacgcttctg
     4021 catggcgaat cggctcggca acaggcactg aaagcgcggg atgccattaa aacgctcact
     4081 ctccttgatg gcgacttcag ccgcaaaaat acaggcggtt gcactattga gttgatccgc
     4141 cgttaaacgc ccttcaatgc cgttagcgcc ttgtttttca acgtaaacat ttacgccgag
     4201 tttgcgtccg gctttttcca gatattccgc agccatatag gtgtgagcaa tacccgccgg
     4261 acaggccgtt acgcagacga tagttggcgc gttggaaaaa gaggcagaag gttgcgtgcc
     4321 tcccttgtca tccagcgccg agagcaactc atcaggcgtc gtcgccgact gtatacgcgc
     4381 ccgaatctca tcatccgcca ggcgcgtggt cagcgctgtc agcagttgca tatgcgtagt
     4441 acccgcttca ttggggggaa tcgccagcag caccactaaa tcaaccgctt ccgggccatc
     4501 aacgccttcc cactgaagcg gctcgctgag tgtggcgacc gcaaacgccg cttcttttac
     4561 cgcagcagtt ttgccatgcg gcacagccaa cccttcacct aaggcggtcg ggccaaggct
     4621 ttcacggcga tacacttctt ccagaaattg ctcagtactg gaaattttcc ccagagcagc
     4681 aagacgttga gtcaacgcgt ggatggcctc ttcacggctg gtaaagcgcg cattcagaca
     4741 caacgcatcg cggtgggtca gagtcgtcag attcatcatg atcctcactt gatcgttttt
     4801 atagtcgcgc cagtgtgccc gataaaacaa taccattatg tgatcaattt cacttgattg
     4861 aaattatttg tattgtattt gtattattta caggatgaat ttatcgggca taatggctaa
     4921 attcgtatta atgagatact aaaaatggga cacaagccct tataccggca gattgccgat
     4981 cgcattcgtg aacaaattgc gcgtggcgag cttaaacctg gcgatgcgtt acctaccgaa
     5041 tcggcattac aaacagagtt tggcgtcagc cgggttacgg tgcgtcaggc tttgcgccag
     5101 ttagtcgagc aacagatcct cgaaagcatt cagggcagcg ggacttacgt caaagaagag
     5161 cgggtcaatt acgatatttt tcagttaacc agttttgatg aaaaactgtc ggaccgtcac
     5221 gtcgatacac acagtgaagt cctgatattt gaagtgattc cggctgacga ttttcttcag
     5281 caacagctac aaatcaccgc gcaggatcgt gtgtggcatg tgaagcgcgt gcgctatcgc
     5341 aagcaaaagc caatggcgct ggaagaaacc tggatgccgc ttgcgttgtt cccggatctc
     5401 acctggcagg tcatggaaaa ttcaaaatat cactttattg aagaagtgaa gaaaatggtt
     5461 atcgatcgta gcgaacagga aatcattcct ctgatgccaa ccgaagagat gagccgtctg
     5521 cttaatatca gccagacaaa gccgattctt gaaaaagtat cgcgtggata tttagttgat
     5581 ggtcgggtgt ttgaatatag ccgcaacgct tttaataccg atgactataa atttaccctg
     5641 atagcgcaac gaaaatcatc gcgataagca caaaaaaggc catcatacga tggccaacca
     5701 tgtcgaaacc gacagccctt cgccattcct gtagagggaa gcggcgaggg ctatttctta
     5761 ttacagatat ttatttcaga acagttttca gtgcttcacc gatatccgcc aggctgcgaa
     5821 cggttttcac gcctgcggct tccagagcag cgaatttctc atccgcagtc cctttcccac
     5881 cggcaatgat ggcacccgcg tggcccatac gtttgccttt cggcgcagtc acaccagcga
     5941 tgtaacccac aactggcttg gtaacatgct ctttgatgta cgctgccgct tcttcttcag
     6001 cgctaccgcc gatctcaccg atcattacga tcgcttcggt ctgcggatct ttttcgaaca
     6061 tttcgagaat gtcgataaag ttagagccag ggatcgggtc accgccgata ccaacacagg
     6121 tcgactgacc gaaaccgtaa tccgtggtct gtttaaccgc ttcataggtc agtgtaccgg
     6181 aacgggaaac gatacccact ttacccggtt tgtgaatgtg acccggctgg ataccgattt
     6241 tgcattcacc cggagtgata acgcccgggc agttcgggcc gatcatacga acgccggctt
     6301 cgtccagttt cactttcaca gtcagcatat ccagcgtcgg gatgccttca gtgatggtga
     6361 taatcagttt gatgcctgca tcaatagctt ccagaatgga gtctttgcag aacggtgctg
     6421 gtacgtagat aacagaagcg gtagcgccag tagcagcaac agcttcacgc acggtgttga
     6481 acaccggcag gccgaggtgg gtagtaccgc ctttacctgg ggttacgccg ccaaccattt
     6541 tagtgccgta tgcaatggcc tgttctgagt ggaaagtccc ctggctacca gtaaagccct
     6601 ggcagataac cttggtgttt ttatcaatta aaatggacat tatttcccct ccactgcggc
     6661 aacaacctgc tgagctgcat ccgtcagacc ttttgctgca ataatattca ggccgctgtc
     6721 agccagtttc ttcgcgccga gttcggcgtt gttaccttcc agacgtacca cgaccggtac
     6781 gttaacaccc acttctgcta ccgcgccgat gataccgtca gcgatcaggt cgcaacgaac
     6841 gataccgccg aagatgttaa ccagaacggc tttcactttg tcgtcagaga ggatgatttt
     6901 gaacgcttcg gttacacgtt ctttggttgc gccgccgcca acgtcaagga agttagccgg
     6961 ttcgccgccg tgcagtttaa cgatgtccat cgtacccatc gccagacctg cgccgttaac
     7021 catacaaccg atgttaccgt ccagcgcaac gtagttcagt tcccactgtg cagcctgtgc
     7081 ttcacgcgga tcttcctgcg actggtcacg catttcgcgc agatcaggct ggcggaacag
     7141 tgcgttgccg tcagcgccca gtttgccgtc gaggcaaatc agatcgccct gtttggtgat
     7201 gaccagcggg ttgatttcga tcaacgccag gtcgcgctcc aggaaaatgg tcgccaggcc
     7261 catgaagatt ttggtgaact gctgaaccag tttaccttcc agacccagtt tgaacgccag
     7321 ctcgcgtccc tgatacggca tcgggcctgt cagcggatca agcgcaactt tatggatcag
     7381 gtgcggagtt tcttccgcca ctttttcgat ttccacgccg ccttcggtgg aggccataaa
     7441 gaccacgcga cgggaactac ggtctacaac ggcaccgaga tacagctctt tagcgatatc
     7501 ggtcgctgct tcaaccagaa tctggttaac cggttggcca ttggcatctg tttgatacgt
     7561 taccagacgc ttgccgagcc agttttctgc aaaagcacgg atgtcttctt tgctgtttac
     7621 aactttcaca ccgcccgctt taccgcggcc accagcgtga acctgacatt tcactaccca
     7681 cgggccggca ccgatttttg aagcggcttc ttctgcttcg cgcggagtag tacaggcata
     7741 acccaccggt gctggtaagc catagcgggc aaaaagttgt tttgcctgat attcatgtaa
     7801 gttcatgtgt tctgtccatc cttcagtaat cgttatcttt taaaccgtag accgacaccc
     7861 tatccacgtc ggtctacagt gcaggtgaag cttaaactac tacacgtcca gcagcagacg
     7921 cgtcggatct tccagcaact ctttgatcgt taccaggaag cccacggatt cgcgaccatc
     7981 gatcagacgg tgatcgtagg acagcgccag gtacatcatc ggcaggatct caacctgacc
     8041 attcaccgcc atcggacgat ctttgatagc gtgcataccc agaattgcgc tctgcggcgg
     8101 gttgatgatc ggcgtagaca tcagggaacc gaacacacca ccgttggtga tggtgaagtt
     8161 accaccggtc agatcttcaa cggtcagctt gccgtcacgg cctttgactg ccagctcttt
     8221 gattttcttc tcgatgtctg ccatgccgag ggtatcgaca tcacgcagta ccggtgtcac
     8281 caggccgcgc ggcgtagaaa ccgccatgct gacgtcgaaa tagttgtggt aaaccacgtc
     8341 atcgccgtcg atagacgcgt tcacttccgg gtaacgtttc agggcttcaa ccaccgcttt
     8401 cacgtagaag gacataaagc ccagacggat gccgtggcgt ttttcaaacg cttcaccgta
     8461 ctgcttacgc agatccataa tcggcttcat gttgacttcg ttgaacgtgg tcagcatggc
     8521 ggtggagttt ttcgcttcca gcagacgctc tgccacacgc ttacgcaggc gagtcatcgg
     8581 gacacgtttt tcactacgtg cagccagagc cggttgcgcc gccggatcag ccgctgccgg
     8641 agcagactct ttcgccgggg ctttcgccag atgtttttcc acatcttcac gagtcagacg
     8701 accacccaca ccggtgcctt taatggcgct ggcgtcgaga ttgtgttcag ccagcaggcg
     8761 acggatcgcc gggcttaacg catcgttgtt ttgctcttcc agagacgcct gctggcgttg
     8821 cgccggagtg gacgctttct cttcagattt ggcgctggtt tctttaccgg cgctgttgcc
     8881 ttcacgcagg cgaccaatga tctgacgaga cgttaccgtt gtaccttcat cttccagaac
     8941 cgcatccaga atgccgtctg ctgatgccgg tacttccagt accactttgt cagtttcgat
     9001 ttctaccagc acttcatcac gtacgactgc gtcgccgggt tttttatgcc aggttgcgac
     9061 ggtggcatcg gctacggatt caggcaggtc agggaccaga atatctacgc tactcattgt
     9121 gtatccttta tttattcgac gttcagcgcg tcattaacca gatcttgttg ctgtttctgg
     9181 tgaacggaca tataccctac cgccggagag gcggaggccg ggcggcctgc ataacgcaga
     9241 gaagccccaa acggaatcac ttcacggaaa tgatgctggc tgcagtacca tgcgccctgg
     9301 ttgagcggct cttcctggca ccagacaaaa tccttgacgt gagcaaactg ctgcaacact
     9361 tcctgcatcg ctttatgcgg gaacgggtag agttgctcga tacgcacaat ggcgacatcg
     9421 tgttgattgt tcttacgacg ctgttccagc aggtcgtaat aaaccttacc agaacacatt
     9481 actacgcgct tcacgccctt cggatcaagc tcgtcgattt caccgatggc tggcaggaag
     9541 gtgccgttcg ccagttcttc gaggctggaa accgccagcg gatgacgcag cagggatttc
     9601 ggcgacatca cgaccagcgg acgacgcatc ccgcgcagcg cctgacgacg cagcatgtgg
     9661 taaacctgtg ccggggtaga cggtacgcaa acctgcatgt tttgctcagc acaaagttgc
     9721 agataacgtt ccagacgcgc ggaggagtgc tccggcccct gcccttcgta accgtgcggc
     9781 agcaacatca ccagaccaca catccggccc catttctgct cgccagagga gatgaactgg
     9841 tcgataacca cctgcgcacc gttggcgaag tcaccgaact gcgcttccca gatggtcagg
     9901 gtgcgtggtt ctgcggtggc ataaccgtat tcaaacgcca gcactgcttc ttcagacagt
     9961 acggagtccc aaacacggaa cgcgccctgg ccgttatgga tgtgttgcag cggcgtgtaa
    10021 gtggaaccgt tagactggtt gtggatcacc gcgtggcggt ggaagaaggt accgcgaccg
    10081 gagtcttcac ccgacaggcg aaccggaatg ccttcgtcaa ccagcgtggc gtaagcgagg
    10141 ttttccgcgc cgccccagtc gaacagtttc tcaccggcag ccatcgcctg gcgatcgcca
    10201 taaatcttgg caacgcgaga ctgcatttca accgcttccg gcaccgtgct gatgcgttta
    10261 gccagttcct gcaggcgctt catctcaact ttgttcgggt actcttcgtc ccattcgtgg
    10321 ttgaggtacg gcgaccaggt gaaagagtgc atgttcatcg gacgccactc tgctaccacg
    10381 caatcgccag catccagcgc atcgcggtac aggttaacca tctcggtggc atcttccagc
    10441 gtcgccactt tttcctgctc cagcttgtca gcgtagattt tgcgcggtgt cggatgtttt
    10501 ttgattttct gatacatcag cggctgggtt gcgctcggct cgtcggcttc gttgtggccg
    10561 tgacggcggt agcataccag gtcgatgaag acatcacgtt taaaggtgtt acggaaatcg
    10621 agcgccagac gggtcacaaa ggcaacggct tccggatcgt ccgcgttaac gtggaaaatc
    10681 ggtgcctgaa ccatcttgcc gatatcagta cagtacggcg tagaacgggc atccagcgga
    10741 ttagaggtgg tgaaaccaac ctggttgttg ataacgatac gtaccgtacc gccaacttca
    10801 taaccacgcg ctttcgacat gttcagggtt tcctgaacca cgccctgtcc ggtcactgcg
    10861 gcgtcaccgt ggatggtaat tggcagcact ttgttgctgc tcggctcatc aagtctgtcc
    10921 agacgggcac gaacagagcc gataacgacc gggcttacaa tctcaaggtg agacgggtta
    10981 aacgccagcg ccaggtgcac caggccgcca tcggtctgga agtcagacga gaagcccatg
    11041 tggtatttca cgtcacccgt gccgaggtgt tctttatgtt taccggcaaa ctcgtcgaac
    11101 aagtcttgcg gttttttacc cagcacgttc accaacacgt tcagacgacc acggtgcgcc
    11161 atcccgagaa ccacttcccg ggtgccgctg ttgccagcgt ggcggatcat ctctttaagc
    11221 atcgggatta acgcgtcacc gccttccagc gagaagcgtt ttgcgccagg gaattttgcg
    11281 ccgaggtaac gttcaaggcc ttcagcggcg gtcagttcgc ttaagaagcg ttttttctct
    11341 tcgctattga aagtcgcgcg accagactcg atacgctgtt ggatccagcg tttttcttcg
    11401 gtgctggtaa tgtgcatata ctcggcacca atcgggccgc agtaggtttg cttgagggct
    11461 tccagcagct cgccgagttt catggtttct ttgccgctgg caaatgaacc gacgttgaag
    11521 gtctcctgga agtctgcttc ggtcagatcg tggaaagacg gatccagatc ggccacttta
    11581 tcttgctgcc acagtcccag cggatcgaga ttcgcatgct ggtgaccacg gaagcggtat
    11641 gcgttaatga gctgcaggac tttaacctgc tttacattgg tgtcagggtc ggagatcgtt
    11701 gaagagtaac gtgaagcgtc tttcgccagg cggcggaaat attcacgcgt ttgagagtgg
    11761 aattgatccg gtttgactcc cgtaccaggt aactgctgga acgtcgaacg ccagttagcg
    11821 tcaaccgagt caggatcggt taagaagtct tcatagagct gttctatcca gctctggttt
    11881 gcgccagaga ggtaagaaga gtccaaccag gctttcaaag cgctgttctg catcgtgatc
    11941 ccttaagcat cttttttatg cttacttcgc cgtggatact accacgcaca gttatgtgcg
    12001 tgccggggtt cacttgcgag ctcttgtcta cgtagtggct cgcgaaggaa cctttaaaaa
    12061 ctgtcttagt gtcaggggca gtttttaaag atttcctgaa tagccggggg atttccccgg
    12121 catcaacatt taattacgca ttacgttgca acaacatcga cttgatatgg ccgatggcgc
    12181 gcgtcgggtt cagccccttc ggacatacac tgacgcagtt catgatgctg tgacagcgga
    12241 atacactgaa tgcatcgctc aaaccgtcga ggcggctgtc agtctcggta tcacggctat
    12301 cgatcaggaa acgatatgcc gctaacaagc ctgccgggcc gataaactta tcgggattcc
    12361 accagaaaga cggacaagag gttgaacaac atgcgcagag aatacattca tacagcccgt
    12421 cgagtttttc gcgctgctct ggcatctgta aatgctcgcg agctggcgga ttttgtccat
    12481 tattcaacag gtaaggctta attttctcat attgcgcata gaattgtccc atgtctacca
    12541 ccaaatcgcg gatcaccggt aaacctggca gcgggcgaat cacaatcttc ttgcccggct
    12601 ggttgagtgc cgaaatcggg gtaatacagg ccagaccatt cttgccgttc atgttcagac
    12661 cgtcggaacc gcacacacct tcacggcagg agcggcggaa cgacaggctg ggatctttct
    12721 ctttcagctg gataagcgca tccagcaaca tcatgtcgcg accttcttcc gcttccaggg
    12781 tgtaatcctg catacgcgga gcatcatcaa catccgggtt atagcgataa attgaaaact
    12841 cgagtctcat tttcctgtct ccgcattagt aagtacgaat cttcggcggg aatgccgggc
    12901 gcagtttcgg ttccatgttg acgcttcggc gcgtcatgga ttccgactct ggcagataca
    12961 gggagtggca cagccagttt tcatcatcac gatccgggaa gtcgaagcgg ctatgcgcgc
    13021 cacggctttc ggtacggaag ttggcagaaa cagccgttgc atacgccgtt tccatcaggt
    13081 tatccagttc caggcactca acgcgctggg tgttgaactc gctggaagtg tcatccagac
    13141 gggcattttt cagacgctcg cggataactt tcaactgctc aagccctttc gccatcgcat
    13201 caccttcacg gaagaccgag aagttatgct gcatacattc ttgcagcgct ttacgaatcg
    13261 ccaccggatc ttcaccgtta cgattattgt tccagcggtt cagacgatcc agagatgctt
    13321 caacatcaga ctcgctggca tcgcgcaatg cgccctgctc ggcgatagac tcttgcagat
    13381 gcagacccgc tgcgcgacca aagaccacca ggtccagcag cgagttgccg cccagacggt
    13441 tagcgccgtg taccgataca caagcgattt caccaacggc aaacagcccc ggaacaacca
    13501 catcttcgcc tttctcgttc accgtcagcg cctgaccggt cactttggtc ggaataccac
    13561 ccatcatgta gtgacaggtt gggataaccg gaatcggctc tttcaccgga tcaacgtgag
    13621 cgaaggtacg ggagagttcc agaatacccg gcagacggga ttcgagaact tctttaccca
    13681 ggtggtcgag tttcagtttc gcatgtggcc cccacggacc atcacagccg cgaccttcac
    13741 ggatttcaat catgatggaa cgcgccacca cgtcacggcc cgccaggtct ttggcgttcg
    13801 gcgcataacg ctccataaag cgttcgccat gtttgttcag cagataaccg ccttcaccac
    13861 ggcaaccttc ggtgaccagt acgcccgcac cggcaatacc ggtcgggtgg aactgccaca
    13921 tttccatatc ctgcaccggt acgcctgcgc ggatagccat gccgacgccg tcgccagtgt
    13981 taatgtgggc gttagtggtg gactgataaa tacgccctgc cccgccagtc gccagcacgg
    14041 tagcgcgggc tttgaaataa accacttcac cagtttcgat gcacagtgca gtacaaccca
    14101 ccaccgcgcc atcctggttt ttcaccagat ccagcgcata ccactcggag aaaatagtgg
    14161 tgtggttttt caggttctgc tgataaaggg tatgcaacag tgcgtgaccg gtacggtcag
    14221 ctgctgccgc agtgcgtgcc gcctgctcgc cgccgaagtt tttcgactga ccgccaaacg
    14281 gacgttgata gatacgacca tcatcaagac gcgagaacgg caggcccatg tgttcgagtt
    14341 ccagaatcgc ttccggcccg gttttacaca tatattcaat cgcgtcctgg tcaccgatat
    14401 agtccgaacc ttttacggtg tcgtacatat gccattccca gttatcttca tgggtattac
    14461 ccagcgcaac ggtaatgccg ccttgcgcag aaacggtatg ggaacgggtc gggaagactt
    14521 tagagagcag cgcacaggtt tgaccgctct gggaaatttg cagcgccgcg cgcatacctg
    14581 cgccaccggc accaatcaca actgcatcaa attctctgac tggcaatttc atcacacacc
    14641 ccacaccaca acgaatccat aaatcacgta aaccaccaga gcaacgacaa tcaccagttg
    14701 cagcatcagg cgcaaggcca gcggtttaac gtagtcggtc aacacctgcc acatgccgat
    14761 ccaggcatgg atcaagatag aaaacagcgc cagcagggtg aacactttgg tgaacgcaga
    14821 ggcgaagaaa ccgatccaga cttcatatgt cagctcgcca ctggtagcga aaaaaccgac
    14881 catataaatg atgtagagcg tcaggacgat agcggtagcg cgaacgagga tgaaatcatg
    14941 tacgccattg cgtcctaatg cggaggcgtt gcttaccata cgaggactcc tgcgagaagt
    15001 gaaagcacga cagtaataac aaaggagatt ttggcggagc gtttacccgc ttcgaatgtt
    15061 tcttccagat agccaaaatc catcatcatg tggcgaatac ctacgacgac gtgatacgcc
    15121 agagcggtaa ggatgcccca catgataaat ttgacgaaga agctgcccat aatcgcggaa
    15181 gcttgctcga aaccttcagg ggaagagagg ctggtaccca gaagccacag caggataccc
    15241 actgcaataa aggtgatcac accggaaacg cgatggagaa tggacgctat cgccgtgatg
    15301 gggaaccgga tggtctgtag gtccagatta acaggtcttt gttttttcac atttcttatc
    15361 atgaataacg cccacatgct gttcttatta ttccctgggg actacgggca cagaggttaa
    15421 ctttctgtta cctggagacg tcgggatttc cttcctccgg tctgcttgcg ggtcagacag
    15481 cgtcctttct ataactgcgc gtcatgcaaa acactgcttc cagatgcgaa aacgacacgt
    15541 tacaacgctg ggtggctcgg gattgcaggg tgttccggag acctggcggc agtataggcc
    15601 gttcacaaaa tcattacaat taacctacat atagtttgtc gggttttatc ctgaacagtg
    15661 atccaggtca cgataacaac atttatttaa tttttaatca tctaatttga caatcattca
    15721 acaaagttgt tacaaacatt accaggaaaa gcatataatg cgtaaaagtt atgaagtcgg
    15781 tatttcacct aagattaact tatgtaacag tgtggaagta ttgaccaatt cattcgggac
    15841 agttattagt ggtagacaag tttaataatt cggattgcta agtacttgat tcgccattta
    15901 ttcgtcatca atggatcctt tacctgcaag cgcccagagc tctgtaccca ggttttcccc
    15961 tctttcacag agcggcgagc caaataacaa acgggtaaag ccaggttgat gtgcgaaggc
    16021 aaatttaagt tccggcagtc ttacgtaata aggcgctaag gagaccttaa atggctgata
    16081 caaaagcaaa actcaccctc aacggggaca cagctgttga actggatgtg ctgaaaggca
    16141 cgctgggtca agatgttatt gatatccgta ctctcggttc aaaaggtgtg ttcacctttg
    16201 acccaggctt cacttcaacc gcatcctgcg aatctaaaat tacttttatt gatggtgatg
    16261 aaggtatttt gctgcaccgc ggtttcccga tcgatcagct ggcgaccgat tctaactacc
    16321 tggaagtttg ttacatcctg ctgaatggtg aaaaaccgac tcaggaacag tatgacgaat
    16381 ttaaaactac ggtgacccgt cataccatga tccacgagca gattacccgt ctgttccacg
    16441 ctttccgtcg cgactcacat ccaatggcag tcatgtgtgg tattaccggc gcgctggcgg
    16501 cgttctatca cgactcgctg gatgttaaca atcctcgtca tcgtgaaatt gccgcgttcc
    16561 gcctgctgtc gaaaatgccg accatggccg cgatgtgtta caagtattcc attggtcagc
    16621 catttgttta tccgcgcaac gatctctcct atgccggtaa cttcctgaat atgatgttct
    16681 ccacgccgtg cgaaccgtat gaagttaatc cgattctgga acgtgctatg gaccgtattc
    16741 tgatcctgca cgctgaccat gaacagaacg cctctacctc caccgtgcgt accgctggct
    16801 cttcgggtgc gaacccgttt gcctgtatcg cagcaggtat tgcttcactg tggggacctg
    16861 cgcacggtgg tgctaacgaa gcggcgctga aaatgctgga agaaattagc tccgttaaac
    16921 acattccgga atttgttcgt cgtgcgaaag ataaaaatga ttctttccgc ctgatgggct
    16981 tcggtcaccg cgtgtacaaa aattacgacc cgcgcgccac cgtaatgcgt gaaacctgcc
    17041 atgaagttct gaaagagctg ggcaccaaag atgacctgct ggaagtggct atggagctgg
    17101 aaaacatcgc gctgaacgac ccgtacttta tcgagaagaa actgtacccg aacgtcgatt
    17161 tctactctgg tatcatcctg aaagcgatgg gtattccgtc ttccatgttc accgtcattt
    17221 tcgcaatggc acgtaccgtt ggctggatcg cccactggag cgaaatgcac agtgacggta
    17281 tgaagattgc ccgtccgcgt cagctgtata caggatatga aaaacgcgac tttaaaagcg
    17341 atatcaagcg ttaatggttg attgctaagt tgtaaatatt ttaacccgcc gttcaaatgg
    17401 cgggttaatt tttatatgcc taaacacaaa aaattgtaaa aataaaatcc actatcggac
    17461 attcatatat tctaaaaaaa aatatatcgg tataaattat aaacaaatcg atgattttta
    17521 atcatctatc ttcctctcag ttacatgaat aaaccaccaa atatagtgca cccctataat
    17581 ttccaatact ttatt
//
LOCUS       DAZFXT010000094        17537 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.094, whole genome shotgun sequence.
ACCESSION   DAZFXT010000094 DAZFXT010000000
VERSION     DAZFXT010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17537
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.094"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(468..1469)
                     /locus_tag="ACHKW0_003434"
     CDS             complement(468..1469)
                     /locus_tag="ACHKW0_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HFO7476211.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWMTFGWKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCALAK
                     TSPGTTYTPIKVSCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPY
                     LLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLASVLMT
                     KGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
     gene            1577..1873
                     /locus_tag="ACHKW0_003435"
     CDS             1577..1873
                     /locus_tag="ACHKW0_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7476212.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1996..2421)
                     /gene="arsC"
                     /locus_tag="ACHKW0_003436"
     CDS             complement(1996..2421)
                     /gene="arsC"
                     /locus_tag="ACHKW0_003436"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008794 - arsenate reductase
                     (glutaredoxin) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HFO7476213.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2434..3723)
                     /gene="arsB"
                     /locus_tag="ACHKW0_003437"
     CDS             complement(2434..3723)
                     /gene="arsB"
                     /locus_tag="ACHKW0_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HFO7476214.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3777..4130)
                     /gene="arsR"
                     /locus_tag="ACHKW0_003438"
     CDS             complement(3777..4130)
                     /gene="arsR"
                     /locus_tag="ACHKW0_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HFO7476215.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4639..4722
                     /gene="dinQ"
                     /locus_tag="ACHKW0_003439"
     CDS             4639..4722
                     /gene="dinQ"
                     /locus_tag="ACHKW0_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible type I toxin DinQ"
                     /protein_id="HFO7476216.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4776..6128)
                     /gene="gorA"
                     /locus_tag="ACHKW0_003440"
     CDS             complement(4776..6128)
                     /gene="gorA"
                     /locus_tag="ACHKW0_003440"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HFO7476217.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6200..7042)
                     /gene="rlmJ"
                     /locus_tag="ACHKW0_003441"
     CDS             complement(6200..7042)
                     /gene="rlmJ"
                     /locus_tag="ACHKW0_003441"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HFO7476218.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7245..9287
                     /gene="prlC"
                     /locus_tag="ACHKW0_003442"
     CDS             7245..9287
                     /gene="prlC"
                     /locus_tag="ACHKW0_003442"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HFO7476219.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9293..10045
                     /gene="rsmJ"
                     /locus_tag="ACHKW0_003443"
     CDS             9293..10045
                     /gene="rsmJ"
                     /locus_tag="ACHKW0_003443"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HFO7476220.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10094..11563)
                     /gene="dtpB"
                     /locus_tag="ACHKW0_003444"
     CDS             complement(10094..11563)
                     /gene="dtpB"
                     /locus_tag="ACHKW0_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HFO7476221.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            11729..11782
                     /locus_tag="ACHKW0_003445"
     CDS             11729..11782
                     /locus_tag="ACHKW0_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476222.1"
                     /translation="MVLNVVLAGDFDCVRRS"
     gene            complement(11881..12315)
                     /gene="uspA"
                     /locus_tag="ACHKW0_003446"
     CDS             complement(11881..12315)
                     /gene="uspA"
                     /locus_tag="ACHKW0_003446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HFO7476223.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12706..13041
                     /gene="uspB"
                     /locus_tag="ACHKW0_003447"
     CDS             12706..13041
                     /gene="uspB"
                     /locus_tag="ACHKW0_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HFO7476224.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13112..14611)
                     /gene="pitA"
                     /locus_tag="ACHKW0_003448"
     CDS             complement(13112..14611)
                     /gene="pitA"
                     /locus_tag="ACHKW0_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HFO7476225.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14843..16045
                     /gene="yhiN"
                     /locus_tag="ACHKW0_003449"
     CDS             14843..16045
                     /gene="yhiN"
                     /locus_tag="ACHKW0_003449"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HFO7476226.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16361..17413)
                     /gene="yhiM"
                     /locus_tag="ACHKW0_003450"
     CDS             complement(16361..17413)
                     /gene="yhiM"
                     /locus_tag="ACHKW0_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HFO7476227.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4578 a         4396 c         4508 g         4055 t
ORIGIN      
        1 ataattacct ttaaattccc tttcatttcc acccggtaat atttgtgtat aaaaaaaggc
       61 agtgatagta tcgtaaagga aaacgttaac aactatcaaa aagcgctcat tttaaaaagt
      121 tattatgttc tccgatagtg tgtgaagtaa tatttttgtg atgttttaga tgtgtaataa
      181 tgaaccaggt taaagtgtat ttaaaaatgt gttgtattag cgactgaaat ctgcctttta
      241 aaaaggagga gggtactgag acaaagagtc attgcttctc cgataaaatg tcagagaagc
      301 atgattacgt ggttattgtc tgttgatgac ttgttgaccg agccgtcatc attctcaatg
      361 cattagttaa atcatttgtg atttttaagg attatcctgt ctatgatttt ctacttagaa
      421 ggatatggca ctaactatgc atcttatagt ttactgctgg catctattta aaatagcaat
      481 tgagtcaaat agcccatcaa gatagccata cctaatataa ccgtcaggaa tgcagctatc
      541 atcggcattc tgaacattga tttaagtaat atcacttccg tcaggcttgc gccggcactg
      601 ccgatgatta atgccattaa tgctcccaga cccattcctt ttgtcatcag aacagatgcc
      661 agagggataa ctgcctcagc ccggatatac agcggaatac cgacaacggc gcttaatggg
      721 atggcgaagg gattatctgc tcctgcatga gcggcaatcc acgctgaggg aataaagcca
      781 taaataaaag atcctattaa tactcctaac aaaagatatg gcagtacatc tttaaactgt
      841 tgcaacgcat ctttggtggc tagttttatg ggattaatac ttactacagc tttggtactg
      901 caacaggtaa ctacggggtt ctcaatggct ttagccgctg gactacaaca actcactttt
      961 atgggcgtat atgtcgttcc cggcgaagtt tttgctagag cacaacaatt tgctgatgag
     1021 cttttactgg caatgatatg acgttcaaac cccagagaat ccaggataat actggcaaga
     1081 acggagacgc cggcggcgat aatcgcgtac aacaaggtga ctttccaacc aaaggtcatc
     1141 cacattagtc cgacgataat gggattaagt aatggtgaaa caaataaaaa agtgagagtc
     1201 ggaccaaacc cggctttcgc tgacaacaat ccacgtaaca tgggtattgt cgagcaacta
     1261 cagaacgggg taacggctcc caacagagca gccaggagat agcctctccc ttttctggcc
     1321 cccatcatct gctggatttt atggtctggc accttttgtc ttatcaggct gacaccggca
     1381 ctaatcacta taaacagcaa cgaaagctcg acggcgagaa acacaaacat ctctgcggca
     1441 tcttgcagca tcgcaagcca actactcata cgtaacctca ttggataaat ctatatttcc
     1501 agaataatcg aaatgaaatt cagatcaaga tatttctggt attatcgaaa tatcaaccag
     1561 acaaaggggg tgtcatatgc agttggaaga agttgctaaa gcgctgaaag agctaggtca
     1621 cccgacccgt ttgttcatat tcaaacacct ggtcaaagcg ggggagcaag gtttgccggt
     1681 cggtgagttg caaaagcagc ttgggatacc cggctctaca ctcagtcacc atatttcagc
     1741 actggtatcg gtagggctgg ttaaacaaaa ccgtgagagt cggacgctga tgtgtgtttc
     1801 gcaatacgca atgctggaag cgataattga attcttacgc gaggagtgct gcgtaaatag
     1861 caaaattgcc tgatgcgcta cgcgctacag atttcatgcc atgcacggtt ttgtaggtcg
     1921 gataagacgc gttagcgtcg catccgacat aaacaaagcg cactttacca actatgggca
     1981 gtggatatcg ccgctttatt tcaaccgctt acccgcttca tcaaccactt tctcgccatc
     2041 ttccttggag aatgcgcctt tttgcgcatc tggcagaatt tccagcacca cttctgaagg
     2101 gcggcacagg cgagttccca gcggcgtcac cacaatcggg cgattaatca gaatcgggtg
     2161 ctgaagcata aagtcgatta accgatcgtc agtaaattta tcttctgcaa ggcccagttc
     2221 ctcatacggc tcgacgtttt tacgcaacag cgcgcgtacg gaaatcccca tatcggcaat
     2281 gagtttgacc agctcatcgc gcgttggcgg tgtttccaga taatagataa tggtcggttc
     2341 tgtaccgctg ttgcggatca tctccagcgt attacgcgac gtaccgcagg ccgggttgtg
     2401 ataaatggta atgttgctca tatcaatatc tcattacaaa gtgaaagaga gacgtagcgc
     2461 cagcgcagcc agcgtcacaa aaagcaccgg cagggtcatg ataatccctg tacggaagta
     2521 atatccccag ctgatagtca tattcttctg cgaaagtacg tgcagccaga gtagcgtagc
     2581 caggctacca attggggtaa ttttcggtcc caaatcgcag ccaatcacat tggcataaac
     2641 catcgcttct ttgataacgc cagatgccgt gctgccatca atggacaacg cgccaaccag
     2701 taccgtcggc atattgttca taatagaaga gaggaaggcg gtgaggaatc cggtgccgag
     2761 cgtcgcggcc cacaggccgt tatccgccag cacgttgagt acgccagaaa gatattccgt
     2821 taatccggca ttgcgcaggc cataaaccac cagatacatg ccgagcgaga agatgacaat
     2881 ctgccagggg gcaccgcgca ggactttacc cgtattaatc gcatgaccgc gtttagcgac
     2941 gacaaataat atcagcgcgc ccacagctgc aatggcgctc accggaatgc cgagcggttc
     3001 caggacgaaa aatcccacca gcagaagcag taaaacaacc cagccagttt tgaacgtagc
     3061 aggatctttg atcgcttctg cgggagattt cagcagcgcc atatcgtagt tctgcggaat
     3121 atctttgcga aaatagagat gtaacatcac cagcgtggca acaatcgcgg cgatatccac
     3181 cggcaccatc accgaggcgt attcgcgaaa gccgaggcca aagaaatcag cggaaacgat
     3241 attcaccagg ttggagacaa taagcggcag gctggcggta tcggcaatga atccggccgc
     3301 catcacgaac gccagcgtag tgcctttact gaaccctaaa gccagcagca tggcgatgac
     3361 aatcggtgtc aaaataagcg ccgcgccatc attggcaaac agggcggcaa cggcagcacc
     3421 gagcaggaca atccaggtaa acagcaagcg accacgacca ttaccccagc gtgagacgtg
     3481 cagcgccgcc cattcaaaaa agccggactc atccagcagc aggctgatga taatgacggc
     3541 gataaacgca gccgtcgcgt tccagacgat attccacacc accggaatat cacccggatg
     3601 gaccacgccc gtaactaacg ccagtactgc gccgagcgtt gcactccagc cgatgcctaa
     3661 acctttcggc tgccagataa ccaatacgat ggtcaggaca aagatagcgc ctgccagtaa
     3721 cataatgcct cccggataaa acacatctga aaattcatat gtgtttagct aaatttttaa
     3781 ctgcaaatgt tcttactgtc cccggaacag ttttgtcgag ccaggttgcg gacaatcgcc
     3841 tgaacctttt cctgttcaca tcgccaggcc tcatcaataa ttttcgccgc ccatgctgga
     3901 atatgcggtg ataagcggta atgaacccac ttaccttgct tgcggtccag caatagcccg
     3961 ctttcacgca gcaatgccag gtggcgggag atcttgggct gcgactggtc gagagcagtg
     4021 cagagatcgc agacgcataa ctctcccagt tcgctgagca gtaaaacgat gcccagacgg
     4081 gtttcatcag caagaatttt gaacaattgg atgggtaaca gaaatgacat attgcgctcc
     4141 tgattgttgc aggtagtgtc tctcttcgaa gcggataagt caaaaacata tatgacttaa
     4201 cgaatgtgta agtgcagggg aaggtaatag gtgtgaattt tgagttggct attcatttga
     4261 aaggaggtct gaatcaggac gtaaaaaaac cgactttgcg tcggtttttt actttccagc
     4321 cctgagttgg tggcactggc tggagtgaga atgccattat ctaacgcatc aatgctaata
     4381 ccatactgaa actattgcaa ggacatgctg gttttataac ctgaatgtac tgtatgatta
     4441 tccagctagc tctgaggcat tttcactctg gtaatgcgca taaacgcttt caaagtcctg
     4501 gtcagaagta cgggtggtgc cgttaactga tgctctggcc ggagtgagaa tgccattatc
     4561 taacaatagg gcatgcgccg tgacaggcag tggatgagta agcggatgca ttctcactcc
     4621 atcgcatgga gaaaacgggt gattgataaa gcaatcatcg ttcttggggc gttaattgcg
     4681 ctgctggaac tgatccgctt tctgcttcag cttctgaact gatagcggaa acgtaattaa
     4741 gggctaagag cacactactc ttagcccttt aacatttaac gcattgtcac gaactcttct
     4801 gccgccgttg ggtgaatggc gacggtattg tcgaagtctt ttttggttgc acccatcttc
     4861 agcgccaccg cgaagccctg caacatttcg tccataccaa agccaatgcc gtgaataccg
     4921 acaatcttct cttccgatcc aacgcacacc agcttcatgc ggcacggctg gcggtgagtg
     4981 gtgacggcgg tatacatcgc ggtgaaagag gatttataca ctttcacctg atcgtcgcca
     5041 tactgctcgc gcgcctgcgg ttccgttaaa ccaacagtac caatcggcgg atggctgaag
     5101 accacggtcg gaatgttgct gtaatccaga tgctcatccg gcttgttatt aaacaggcgt
     5161 tcagagagac ggcgacccgc tgcaactgcc accggtgtca gctccactgc acccgtgtta
     5221 tcgcccaccg cgtaaatacc ttcaatattg gtgttttgat atttatcgac gacgatatag
     5281 cctttttcgt tagttttaac gccagcggct tccaggttga tgttgtcatt ggcaggctcg
     5341 cgaccaatcg cccaaatcag gcaatccacc gtttcactgc gaccatcttc cagctccagc
     5401 gtcaggctac catcggcatt tttcactacc gctttcggga tggcgttggt gtgcagctgc
     5461 gggccttcgg cgttcatcac ttcgaccagc gtttcggaaa tcatcgggtc gaagctgcgc
     5521 agcggcgcat gtttacgcac aaacagatgc gttttcgcgc cgaggccgtt aatcacgccc
     5581 gccagctcaa cggcgatgta acccgcgcca acaaccgcca cgcgctctgg caaagcagga
     5641 agggcgaaga agccatcaga atcaataccg tattccacgc ccggaatatc cgggtggctc
     5701 ggacgaccgc ctgtggcgat cagaatatga tcggccgtga tggtttcgcc gtttacctcc
     5761 agcgttttgg catcaacgaa gcgggcaaag cctttgatta catcaacgtt atttttaccg
     5821 agcacgtttt cataggaagt atgaatacgg tcgatatagg cggtacggct ggcgatcaac
     5881 gtttcccagt tgaatttatt gatagtggta tcaaaaccat aatccgggcc gtacatatgg
     5941 atcgcttcac ggatttgcgc cgcgtgccac atcacttttt tcggcacaca gccgacgttc
     6001 acgcaggtgc cgcccagctc tttggcttca atcagtgcgc atttctggcc gtacatagcc
     6061 gcgcggttga tggaggctat accgccgctg ccgccgccga tggcgatgta atcatagtgt
     6121 ttagtcatga cagagtgtcc ttatcgttga ttaccgcgat tgtagcgcga gacgtaatag
     6181 gtgccagcaa tggctgcaat tactccggca cgatccagct tacggtggcg tgcccggtgc
     6241 ctgccggaac cagtttgctg tgcagccacg gcagcacgtt attcatctgt tgttccagtt
     6301 tccacggcgg gttaatcaca atcatgccgg aagcggtcat gccacggcga tcgctgtctg
     6361 gcagtaccgc cagttcaatt tgcagaattt tgcgaatacc ggtcgcttcc agatcgtgga
     6421 tcatgcgctt aatttgctga cgcagcacca ccggatacca cagtgcgtaa gtaccagtgg
     6481 cgaaacgttt gtaaccttct gctatcccgc tgaccaccgc ctgatagtcg gttttcattt
     6541 catacggcgg gtcgataagg attaaaccac ggcgggaaac cggcggcagt ttggccttaa
     6601 gctgctggaa accatcggct ttttcgacgc gcgcacggct gtctttctga aattcagaac
     6661 gcagcaacgg gtaatcgctc gggtgcagtt cggtcagttg caggctgtct tgttcgcgca
     6721 gtagctggcg agcaatcaac ggcgaaccgg ggtagtaacg caactgaccg ctacggttga
     6781 agtgttttac cacattgatg tacgcctcca gttctgcggg caaatcgtcc tgctgccaga
     6841 tacgggcgat gccttcgaga tattcgccgg tacgctcggc atgttcgctg cctaactgat
     6901 aacgcccggc ccctgcgtgg gtgtcgagat agagaaacgg tttatctttc tctttcagcg
     6961 actcgatgat caggctctga acggtatgtt taaggacgtc ggcgtggttg ccagcgtgaa
     7021 agctgtggcg ataactgagc atgggtaaag gtgttccggt aagtaaaaat cggcccgtat
     7081 tcggggcgca caaaagcgta tcaggacagt ataccgaaaa gaggccgccg ccgcgaaagc
     7141 gtaacgtttc tcattgaaat tcactacact taaccccatg ctacacacat tatgtaaagc
     7201 gcctgttgag cgcttcctta acctctttaa ccaggactgc gcgaatgacg aatccgttac
     7261 tgactccctt tgaattgcct ccgttttcta aaattctccc ggaacatgtc gttccagccg
     7321 tgactaaggc attgaacgac tgccgcgaaa acgtggagcg cgtagtagcg caaggggcac
     7381 cgtacacctg ggaaaatctc tgccagccgt tggcggaagt ggacgatgtg ttggggcgta
     7441 tcttctcccc ggtcagccac ctgaactcgg tgaaaaatag cccggaactg cgtgaagcgt
     7501 acgaacaaac cctgccgctg ctgtcggaat acagcacctg ggtagggcaa catgaagggc
     7561 tgtataaagc gtaccgcgac ctgcgcgatg gcgatcatta cgccacgctg aacacggcgc
     7621 agaagaaagc ggttgataac gccctgcgtg attttgaact ctctggcata ggtctgccga
     7681 aagagaaaca gcagcgttac ggcgaaattg ctacccgtct ttctgaactg ggcaaccagt
     7741 acagcaacaa cgtcctcgat gcgacaatgg gctggaccaa actcgttacc gacgaagcgg
     7801 aactggcggg gatgccagaa agcgcgctgg ctgcggcaaa agcccaggct gaagcgaaag
     7861 aacttgaagg ctacctgctg acgctggata tcccaagcta cctgccggta atgacctact
     7921 gcgacaacca ggctctgcgt gaagagatgt atcgcgctta cagcacccgc gcctccgatc
     7981 aaggtccgaa cgccggtaag tgggacaaca gcaaggtgat ggaagagatc ctcgcgctgc
     8041 gtcacgaact ggcgcaactg ctgggctttg aaaactacgc ctttaaatcc cttgccacta
     8101 aaatggcaga aaacccgcag caggtgctgg atttcttaac cgatctggca aaacgcgcgc
     8161 gtccgcaagg cgaaaaagag ctggcacaac tgcgcgcctt tgccaaagcc gaatttggcg
     8221 tcgatgagtt gcagccgtgg gatatcgcgt actacagcga aaaacaaaaa cagcacctct
     8281 acagcatcag tgacgaacag ctgcgtccgt acttcccgga aaacaaagcg gttaacggtt
     8341 tgtttgaagt ggtgaaacgt atttacggca tcaccgctaa agagcgtaaa gatgttgatg
     8401 tctggcaccc ggatgtacgt ttcttcgaac tgtatgacga gaacaacgaa ctgcgcggta
     8461 gcttctacct cgacctgtat gcccgtgaaa acaagcgcgg cggggcgtgg atggatgact
     8521 gcgtaggcca gatgcgcaaa gctgacggtt ctctgcaaaa accggtcgcg tatctgacct
     8581 gtaactttaa ccgcccggta aatggtaaac cggcgctgtt tacccatgac gaagtgatca
     8641 ccctgttcca cgagttcggt catggtctgc atcatatgct gacccgcatc gaaaccgccg
     8701 gtgtatccgg gatcagtggg gtgccgtggg atgcggtcga actgccgagt cagtttatgg
     8761 aaaactggtg ctgggagcct gaggcgctgg cgtttatctc tggtcactac gaaaccggcg
     8821 aaccgctgcc gaaagagttg ctggataaaa tgctggcggc gaagaactac caggcggcgc
     8881 tgtttattct gcgccagctg gagttcggcc tgttcgattt ccgcctccat gccgagttcc
     8941 gcccggatca gggagcgaaa atcctcgaaa ctctggcaga aatcaagaaa ctggttgccg
     9001 tagtaccgtc tccatcctgg ggccgtttcc cgcacgcttt cagccatatt ttcgccggtg
     9061 gttatgccgc aggttactac agctacctgt gggctgacgt actggcggca gatgctttct
     9121 cgcgctttga ggaagagggc attttcaacc gtgaaaccgg gcagtcgttc ctcgacaaca
     9181 ttctgagccg tggcggttca gaggagccga tggatctgtt caaacgcttc cgtggtcgtg
     9241 agccgcagct ggatgcgatg ctggagcatt acgggatcaa aggttagttt ttgtgcagat
     9301 ttgcttaatt gatgaaacag gcaccggaga cggtgcctta tctgttctgg cggcccgctg
     9361 ggggctggag cacgatgaag acaacctgat ggcgctggtg ttaacgccgg aacatctgga
     9421 attgcgcaag cgtgatgagc caaaacttgg cggcatcttt gttgattttg ttggcggagc
     9481 gatggcgcac cgacgcaaat tcggcggtgg tcgcggtgag gcggtggcga aagcggtggg
     9541 cattaaaggc gattatttgc cggatgtggt ggacgccacc gcagggctgg ggcgcgatgc
     9601 ctttgtgctg gcttcagtcg gttgccgcgt gcggatgctg gagcgtaatc cagtcgttgc
     9661 cgccttgctc gacgacggcc tggcgcgtgg ttatgcggat gcggaaatcg gcggctggtt
     9721 gcaggagcgg ttgcagttaa ttcacgcctc cagcctgacg gcgctgactg atattacccc
     9781 gcgcccgcag gtggtttatc tcgacccgat gttcccgcat aagcagaaaa gcgcgctggt
     9841 gaaaaaagag atgcgtgtct ttcagtcgct ggtggggccg gatcttgatg ccgatggatt
     9901 gctggagcct gcgcggctgc tggcgaccaa acgcgtggtg gtaaagcgcc cggactacgc
     9961 gccaccgctg gcgaatgtcg ccacgccaaa cgcggtagtc actaaagggc ataggtttga
    10021 tatttatgcc ggaacgccgg tgtaaaagta aagcagggta gcgataacgc taccctgctt
    10081 tctgcaagaa taattaatgg ctttccggcg tcgcaatcat gcgtttcagc cacggcacca
    10141 tcaacagcat cactactgca acgcccagcg tgaccagacc aatcttacca aacacgttgg
    10201 tatagacggg caacgtctca agcggatcgg taatgttgtc cggcaccgcg gtaaatgttg
    10261 ccacatagcc gcccagcaag aacgcggcag cctgcgtcag gaaccacatc ccgagaataa
    10321 agcccatcaa atgctgcggc accagggcag caatcatcgc cagacccagg gcgctaataa
    10381 acaattcacc caggctctgg aacaagtaaa ccagcacgat aaaccacggc gatgtcagtc
    10441 cttgtgcatc agcaaaccac attcccgcag ctgccgccgt caaaaagccc agtgagcaca
    10501 taaacatgcc gagagtaaat ttcatcggca tcgagaggtc tttgcctttg ttacccagat
    10561 gcgtgtaaat gcctgccagt attgggctgg cgagtaccac ccagaacggg ttaagcgcct
    10621 ggaagctgac cgggttgatg gaaaagccga gaatttcatg atgcacgttg ttgatggcaa
    10681 agaagttcag cgacgttggc atctgggcgt agagaatgta aaacaccacc gcttcgagca
    10741 tcaggacaaa ggcgacaaac attttattgc gcccggtttt atccagcttg aatgcctgac
    10801 gaaagaagat gatggtgacg acgatggaga gaacaatcag caccagattg gcgacttcta
    10861 cgttgtgcat cagccatgca cagacgaaga tcatcaccac gctgccaagt aacacgtaca
    10921 acagtttgct gaagctcatt ggccggaagt cgggttcaga accaatgtct ttcaccattc
    10981 cacgacaggc gatgtaaacc agtaatgcga taattaaccc cgccccgcac aggttgtagg
    11041 tgactgaata accgaatcta tcagcgatca caggggccag cgataacgct atcaacgagc
    11101 cgatattgat cgacatatag aacagggtga atgcgccatc aagccgcgga tctttcggcg
    11161 gatagcactt cgaaagcaag ctggctgggt tagctttaaa caggccgtta ccgacagcga
    11221 tagtccccag ggcgatgaaa atcaggtcag gcttaagtag cgacatgccg gtcatgaagt
    11281 agccaatcgc cagcacaagt gctccgagaa caatggtgcg tttggtcccc agcaggtggt
    11341 cgccgacata gccgccaatg gaaatgaggc catagaccag cgcagcaaaa gcaccaaaag
    11401 tgacaaaagc ctgctcttgc gagaatccaa gctgtttaac gaagaaaacc gccagtacgc
    11461 cctgcacgcc gtagtagccg aatcgctccc ataactcgac aaaaaagatc atgaaaaatg
    11521 ggcgaggttg ctgcagcatc cccatgggtg ttgttgtatt catatttatt aaccttccaa
    11581 taccatcctg aaaagtatga acgcgtaagg gataacgcgc gaaaaagttt acatctgtca
    11641 gagattctta atattgatgt ggtgaataga ttaatacact attacaacag aaaataacca
    11701 gctgatcaca ctaatgatgg aaaatatgat ggtgttaaat gttgtattgg caggagattt
    11761 tgattgcgta cgcagatcgt aggccagata aggggtttac gctgcatcag gtaacaaggc
    11821 gcgccaaaaa gcaaaacgcc cgcatatgcg ggcgttcagg aacacgtcgt ggggggatga
    11881 ttattcttct tcgtcgcgca gcggaacaat cagcatatca acgtgaacgg tgttgatcag
    11941 ctgacgtgcg gaagacatta gtttgctcca gaagtcctgg tggtgaccac aaaccaccag
    12001 atccatatcg tatttcttga ttgcatcgac cagaacctgg cccaggtcgc cgctgccgct
    12061 cagggtttca gtgattgggt agcctgcatt agtggaaagc tcggtcagcg catgatgtgt
    12121 ctcttcagag atgcgtttct gcatatcacc cagattcaca tcaataagcc cggtgtatag
    12181 gtcagagtag tttacatcta cgtggatcag agaaactttc gcattgtagg ggcgagccat
    12241 agagactgct ttctctacca gaactttgct ttccggggag agatcgaccg cgatgagaat
    12301 gtgtttataa gccatagtgt tactccttcc ataaagttgt cgatgactgg ccagctagcg
    12361 tttcttgtgc cgttacgtca cccgcgtcct gcgaactacg tgcgcggggc taacccagcc
    12421 gtaaagattc agtgttaaga ccatccttac cttatagcga cccggatgat ccgtcaatcc
    12481 gccttgctta cccgttagat aaacaaattt tcgacatgaa tatattgata gtggttaacc
    12541 ttctggaaaa aaaacaacct gatctcctac actatctata gagccgctcg gatgttggtc
    12601 accacaaaag cggtttctcc aggacctggt gttaactgat tggcagcgta tcgggagcgg
    12661 tagccccggg gtggaactcc gcgggcaggt cgccggggag gaggtatgat aagcaccgtc
    12721 gcattatttt gggctttatg tgtcgtttgc attgttaaca tggcgcgcta tttctcatca
    12781 ctacgcgcgt tgttagtggt actgcgtaac tgcgatccat tgctctatca atatgttgat
    12841 ggagggggct tttttacctc acatggccaa cccaacaaac aggtgcgtct cgtttggtat
    12901 atctatgccc aacgttatcg cgatcatcac gatgatgaat ttattcgccg ctgtgagcgg
    12961 gtgcgtcggc agtttattct gaccagcgca ttgtgtggtc tggtggtggt cagcctgatt
    13021 gcattgatga tttggcatta agcacttcag caataataac gcataaaaaa agcgggccag
    13081 taaactgacc cgctcgtttt ggtgtgtacg attacaggaa ctgcaaggag agccagtaca
    13141 gtccgccgga aagcagtacc gcagccggaa gggtaaacac ccaggccatc agaatactgg
    13201 ttacggtttt acgctgtaag ccgccgccat ctaccaccat cgtccccgcg acagaagagg
    13261 agagtacgtg agtagtggaa accggcatcc cggtataact cgccaggccg atagacactg
    13321 ccgccgtcat ctgggcagac atcccctgag cgtaggtcat gcctttctta ccgattttct
    13381 caccgatagt cgttgccaca cggcgccagc caatcatcgt accgatacct aacgccagcg
    13441 cgaccgccat gatgatccac accggtgcat actcgatggt gctaagcatg tcggacttca
    13501 gtttcttcaa caggcgctga tcgtcagcac tcacgccagg catcttcacc actttgtcga
    13561 tagtgtcaga aacgcacagc ataatgcggc gcatctggct acgttgatca agcgacagct
    13621 tgtcgtagct ttccacatcg gtggtcaaca tacctttcag gcggttgagc gcgttaatgg
    13681 tattcgacgg atggcagtgg aactccgcag gttgcgttgc gccagcttcc ggagccggta
    13741 ctaactgatc agcaccggta gcctgtttga gcagcgcagg atgctgctca aagtaagctt
    13801 cgacgttgtt gatggcatca cgggtacggg tgatttcgta gccagtggca ttcatgttca
    13861 ccacgaagcc tgctggcgcg acgccaatca ataccaacat aaccagacca atgcctttct
    13921 gaccatcgtt cgcgccgtgc gaaaacgcca cgccgatagc ggaaaggatc agcgcgatac
    13981 gcgtccagaa cggcggcttt ttcttgccgt ctttcttttc acgctccgct ggggtcaggt
    14041 ggatacgggc gcgtttcttg gtgccgctcc agtagcgacg cagcaagaaa atcagaccgc
    14101 cagcaaacac caggccgaca ataggggaaa cgatcagaga accgaaaata cttaatactt
    14161 tcgggatatt gagtgcatcc accactgacg tcccggtcat caacgcattg gttaaaccaa
    14221 tcccgatgat cgcgccaatc agcgtatgag agctggatgc aggtaaacca aagtaccagg
    14281 tacccaggtt ccagataatc gccgccagca acatagagaa caccatggca aggccatgag
    14341 acgatcccat attaagcagc agatccgtcg gcagcatatg cacaatggca taggcaacac
    14401 tcagaccacc cagcaaaaca cccaaaaagt tgaacaccgc cgccataacc acggcgagct
    14461 gagaacgcat cgcgcgggta tagataacgg ttgccacggc gttggctgtg tcatggaaac
    14521 cattgatggc ttcgtagaac agcacaaaag ccagtgcaag caataataac agcccggtat
    14581 gcaaatccag gccagcaaac aaatgtagca taggacgtta cgccattttg aggacatgaa
    14641 cgcggcgcat tatcagtgac tttcgcggcg cgggcaaagt gaaatataga ctttttttga
    14701 tttgcctcct gtatggattt cactcaaaaa ataattatct tatataattc aggcaaatac
    14761 ttccttttag taatattgat gctggtgcga ccactgagga atctttacaa ttcacgcccg
    14821 ttttttctaa gaggagcgca acgtggaaag gtttgatgcc attattatag gcgctggtgc
    14881 ggcgggtatg ttctgttctg cgctggcagg tcaggcagga cgccgggttc tgctgatcga
    14941 taatggtaaa aaaccagggc gcaaaatcct tatgtctggc ggtgggcgct gcaactttac
    15001 caacctttat gtcgaaccag gcgcttatct gagccagaat ccgcattttt gtaagtctgc
    15061 actcgcgcgt tttacccagt gggatttcat tgatctggtc aataaacacg gcatcgcctg
    15121 gcacgagaaa acgttaggac aactcttctg cgatgactcc gcgcagcaga ttgtcgacat
    15181 gctggtggat gagtgcgaga agggcaatgt aaccttcaga ttgcgtagcg aagtgctgag
    15241 tgtggcgaag gatgaaacag gcttcacgct tgaactgaac ggcatgactg tcggttgcga
    15301 aaagctggtc atcgcgaccg gtgggctgtc aatgccgggg ctgggcgcgt cgccgtttgg
    15361 ttataagatt gccgaacaat ttggcctcaa cgtgctgccg acccgcgcgg gtctggtgcc
    15421 attcactctg cataaaccgt tgctcgaaga gttacaggtg ctggcgggcg tggcggtgcc
    15481 ttccgtgatt accgctgaaa acggcatcgt tttccgtgag aacttactct tcacccatcg
    15541 cggcttgtct ggaccggcgg tgttgcagat ttccagctac tggcaaccgg gtgaatttgt
    15601 cagtatcaat ctgctaccgg atgtggatct cgaaaccttc ctgaatgagc agcgtaacgc
    15661 acatccgaat cagagcctga aaaacacact ggcggttcat ctaccgaagc ggttggttga
    15721 acgcttacag caactcgggc aaatcccgga tgtttcgcta aaacagctca acgtgcgtga
    15781 ccaacaggca ctgattagca cattgaccga ctggcgcgta caacccaacg gcactgaagg
    15841 ctatcgcact gccgaagtga cgctcggcgg cgtggacacc aacgaactct cttcacggac
    15901 gatggaagcg cgcaaagtgc ctgggctgta cttcatcggc gaagtgatgg acgttaccgg
    15961 ctggctgggg ggctataact tccagtgggc gtggtcgagt gcgtgggctt gtgcgcagga
    16021 tttgattgca gcgaagtcgt cctgacctgt tacctaaaga tgaaaattat tagagccgta
    16081 ttaaaaatga gctaattttg atagtggcta tcttgtgatt attttctaat gagcccgtga
    16141 actgaaaccc tccaggctta atataaggtg gaaggaaagg tgattgaaaa ctcacccagt
    16201 ggccagaact ttataaaata tgaacaggtt gaaaaaaaca gtaactttgt tgttttttta
    16261 tccttaacca ctatgcatta atgctgcgtt atttcatgat gcctaagaaa aaccagcgtt
    16321 acgcaaatgg tcaacgctgg ttttatccgg tacgttgcaa ttatttttta gcagaacctg
    16381 cttctaatat tgaaacgatt gagaacaacg taaagcatac cgctcctaaa ccgaccaaaa
    16441 cccgcgaagg aataaaatat cccatgtcgg tctgcgccat ttccgcaaga aacgatgcca
    16501 ggaaaaggca aaacaggcag gtgaagacgg gaatcatcgg tattctgttg gctaacgaac
    16561 aggtacgtct ccataccagt gccagtagcc agacttttga gaatatgctg taacagatca
    16621 ttccgaggca aataagaata atgccgggag ccagtcgggc gcttgcatcg gaactgacta
    16681 agacgtatat cccctgcagt accgtgattg agccgagaaa aataacccaa taacaccaca
    16741 gccagtgttc tttagttgaa aacgtattac gtgtttgatg gacaattgtg gcaacaaggc
    16801 caattagaca ggcacagatt gcggttaacc caagcaatac gtgacccgcg acataatgcg
    16861 gagttatgtc agcactacgt aacagcgtaa tagaccaaat gaaaccgagc agggtgagta
    16921 aaacgggaac ggcaatgagg caattaccga ttaatgaaga ataagcctgt accggcgttc
    16981 catcgctctt gctccccgct gcatttttgg gaatgagcag aaagtgaccg gatgacgctg
    17041 ccaccgtcga tacacaggcg gcaatcatac caacgccgaa aataacatgg ccggcgacaa
    17101 actcgtctgc catcacatca ttgcctgcta acagtgccca accccatatc atggtgataa
    17161 ttgaccccgc atagccaata atggggaaca atgtattgta aaacgtatta acgccgcgcg
    17221 tgagctgcga aatgataata aatgcggtag tgaataatgc caggcatatg gccgccagag
    17281 aaatcagcac atgacccgcg acgaaatact cgctttgccc tgaactttcc agcacaaagc
    17341 cacctaacgc gatgcaaatt aatcccataa tcaaagggat taatttgaaa agccacccga
    17401 tatatatgtt cactttaaac tcctcaggta tccctgctaa taatgaaatg tgcattaatg
    17461 ataatttcag cctgttaata taacgacgag atatcattaa agacaaatgc atagcataac
    17521 tatagcgggg tttactg
//
LOCUS       DAZFXT010000095        17528 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.095, whole genome shotgun sequence.
ACCESSION   DAZFXT010000095 DAZFXT010000000
VERSION     DAZFXT010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17528
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.095"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..466)
                     /gene="yehS"
                     /locus_tag="ACHKW0_003451"
     CDS             complement(<1..466)
                     /gene="yehS"
                     /locus_tag="ACHKW0_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HFO7476228.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPVLEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKK"
     gene            complement(513..1232)
                     /gene="btsR"
                     /locus_tag="ACHKW0_003452"
                     /gene_synonym="yehT"
     CDS             complement(513..1232)
                     /gene="btsR"
                     /locus_tag="ACHKW0_003452"
                     /gene_synonym="yehT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HFO7476229.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(1229..2914)
                     /gene="btsS"
                     /locus_tag="ACHKW0_003453"
     CDS             complement(1229..2914)
                     /gene="btsS"
                     /locus_tag="ACHKW0_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HFO7476230.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            3136..3867
                     /gene="mlrA"
                     /locus_tag="ACHKW0_003454"
     CDS             3136..3867
                     /gene="mlrA"
                     /locus_tag="ACHKW0_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HFO7476231.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDILPLGI"
     gene            3927..4034
                     /gene="yohO"
                     /locus_tag="ACHKW0_003455"
     CDS             3927..4034
                     /gene="yohO"
                     /locus_tag="ACHKW0_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HFO7476232.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(4015..4746)
                     /gene="yehW"
                     /locus_tag="ACHKW0_003456"
     CDS             complement(4015..4746)
                     /gene="yehW"
                     /locus_tag="ACHKW0_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HFO7476233.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(4751..5677)
                     /gene="yehX"
                     /locus_tag="ACHKW0_003457"
     CDS             complement(4751..5677)
                     /gene="yehX"
                     /locus_tag="ACHKW0_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HFO7476234.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALVEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            complement(5670..6827)
                     /gene="yehY"
                     /locus_tag="ACHKW0_003458"
     CDS             complement(5670..6827)
                     /gene="yehY"
                     /locus_tag="ACHKW0_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HFO7476235.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            complement(6834..7751)
                     /gene="osmF"
                     /locus_tag="ACHKW0_003459"
     CDS             complement(6834..7751)
                     /gene="osmF"
                     /locus_tag="ACHKW0_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HFO7476236.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            complement(7962..10259)
                     /gene="bglX"
                     /locus_tag="ACHKW0_003460"
     CDS             complement(7962..10259)
                     /gene="bglX"
                     /locus_tag="ACHKW0_003460"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HFO7476237.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLINS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            10455..12170
                     /gene="dld"
                     /locus_tag="ACHKW0_003461"
     CDS             10455..12170
                     /gene="dld"
                     /locus_tag="ACHKW0_003461"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_process: GO:0006089 - lactate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HFO7476238.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(12208..13140)
                     /gene="pbpG"
                     /locus_tag="ACHKW0_003462"
     CDS             complement(12208..13140)
                     /gene="pbpG"
                     /locus_tag="ACHKW0_003462"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HFO7476239.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATAASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(13314..13901)
                     /gene="yohC"
                     /locus_tag="ACHKW0_003463"
     CDS             complement(13314..13901)
                     /gene="yohC"
                     /locus_tag="ACHKW0_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031267 - small GTPase binding [Evidence
                     IEA];
                     GO_process: GO:0006888 - endoplasmic reticulum to Golgi
                     vesicle-mediated transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Yip1 family protein"
                     /protein_id="HFO7476240.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            14071..14649
                     /gene="yohD"
                     /locus_tag="ACHKW0_003464"
     CDS             14071..14649
                     /gene="yohD"
                     /locus_tag="ACHKW0_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HFO7476241.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVAFGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(14779..15540)
                     /gene="yohF"
                     /locus_tag="ACHKW0_003465"
     CDS             complement(14779..15540)
                     /gene="yohF"
                     /locus_tag="ACHKW0_003465"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7476242.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(15593..17029)
                     /gene="mdtQ"
                     /locus_tag="ACHKW0_003466"
     CDS             complement(15593..17029)
                     /gene="mdtQ"
                     /locus_tag="ACHKW0_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HFO7476243.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVDGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAGSNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            17253..17336
                     /gene="yohP"
                     /locus_tag="ACHKW0_003467"
     CDS             17253..17336
                     /gene="yohP"
                     /locus_tag="ACHKW0_003467"
                     /inference="COORDINATES: protein motif:HMM:NF011345.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YohP"
                     /protein_id="HFO7476244.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
BASE COUNT         4217 a         4831 c         4395 g         4085 t
ORIGIN      
        1 ttttcttcac atgctggcgc gctgccagtc cacgcagaaa ataacgtaaa aattgatcgc
       61 cgcattcgcg gaagttttta tgatccggtg cgcgcatcat cgctgtaatt tccggcatcg
      121 aaacgcggaa ctgctgttcg gtgaggatag ccagaatgtc atcggttttc agcgaaaacg
      181 cgatgcgtaa ttttttcagc acgatgttgt tattaatgcg acgttccggc tccagtaccg
      241 gagcagactc atccttgccg cgtttttcat aaatcaggcc attgaggaat gacgacaaaa
      301 caatgtccgg acaacgctga aaaccctctt cgtcttcttt acgtagccag acggcgatct
      361 gttccgcggt ggcttcgaca ttacccagcg ccagaatacg caccaggtca ttattattgg
      421 ctttcaaaat gtagcgcacg ctgcgcagaa tatcgttact tagcatgagg ccttcaggtg
      481 ttgatgaggc aaaaagccat tttagcagtc ttttacaggc caatcgcctc ttttaagctt
      541 ttcagataac ggcggctgac cggcacggtt aagccattac gcaaaatcaa ctcggcctgg
      601 ccgttatctt ccagacgaat ctcctgtaaa tgcgcgaggt taaccagata ctgacgatgg
      661 cagcgcagta gtggtgtacg actttccagg gtacgtaatg tcaattcggt aaagccctct
      721 ttcccttcgt ggctggtaac gtagacaccg ctcatccgac tgctgacaaa tgccacatct
      781 ttcatttgca gcaaataaat ccgactatgc cccgtacaag ggataaattt cagcgcctgt
      841 tgattttccg gtaacagcga aacatcctgc ttgctgcgct cctgacgcaa tcgcgccagc
      901 gttttctcca gtcgcgcttc atcaattggc ttcagcagat aatcaaaggc atgttcttca
      961 aaggctttaa tagcgtattc gtcaaacgca gtgagaaaaa caatatacgg gcgatgttcc
     1021 gggtcaagca tcccaaccat ttccagacca ctgatgcgcg gcatctggat atcgagaaac
     1081 agcacatccg ggcgcagttt atgcaccgcg ccgatccctt ccacggcgtt tgaacactct
     1141 ccaacgattt caatatcgct ctgctcctgc aaaaatacac gcaggttctc ccgtgctaac
     1201 ggttcatcat cgacaattaa gactttaatc atgcctcgtc cctccatggt agtcgtaacg
     1261 ttattcgggt gtaactatca ggctcacagg cgacgcttat tccatagtca tcgccaaacc
     1321 gttcacgtaa acgcttatcc accagattca tccccagccc actggcattg gttaccggtt
     1381 gatacaaacc ggcattgtct tcgatctcca gcatcaaatg ttgcccctca cgtcgggcgc
     1441 tgattgccac tcgccctgta tccagcagtt gtgatgtccc atgtttaatg gcgttttcca
     1501 ctatcggttg cagggtaaac gcgggcaatt gctgctggga taattcttgc ggaatagcaa
     1561 tgttgacctg caaccgcgac tggaagcgcg ccttttcaat ttgcagataa gcgttcacat
     1621 gttcaatttc gtcggcgaga gtaacaaact ccgaaggccg ctttaagttt ttgcggaaaa
     1681 aagtggaaag atactgcacc agctggctgg cctgttcgct gtcgcggcgg atcaccgctt
     1741 taatggtgtt aagcgcatta aacaaaaaat gaggattcac ctgggcgtga agcagtttga
     1801 tttctgactg ggtgagcatc gctttttgcc gctcatattg cccggcaaga atctgcgccg
     1861 aaagcagttg cgcaatcccc tcgcccagcg tgcggtttat tgaactgaat aaacggtttt
     1921 tggcttcata caatttgatg gtgcccatca cgcgctgatt ttcgccacgc aacggaatta
     1981 ccagcgtcga ccccagtttg cattgcggat gcaaagagca acgataaggt acttcgttgc
     2041 catcagcgta gaccacttca ccggtttcaa tcgcttttaa ggtgtaagtc gaagaaatcg
     2101 gtttgccggg taaatggtgg tcgtcaccaa ttccggtaaa ggccagcaat ttctctcgat
     2161 cggtaatcgc gactgcacca atatccagct cctgatacag cacctgagcc actttcatgc
     2221 tgttcacttc gttaaacccc tgtcgcaaaa tgccttccgt cgaggctgcc actttcagcg
     2281 cagtggcaga aaaagcagaa gtgtattttt caaacatcgc gcgtttatcg agcaatatac
     2341 gcataaacag cgccgcgccg acggtattgg tgaccatcat tggtgcagca atattactca
     2401 ccagacgcac cgcatcttca taaggtcggg cgatcgcaag gatgatcagc atttgcacca
     2461 tttcagcgac gaacgtgacg gcaccggcgg taatggggtt aaagacttta tcagtgcgcc
     2521 cgcgacggat caggatgctg tgtaccaggc cgccgagcaa tccttcaacg atggttgaga
     2581 tcatgcaact tagcgcggtc atgcccccca tcgaatatcg atgtaagccg ccggtcagac
     2641 caaccagccc accgacgacc ggaccgccga gtaagccgcc cattaccgcg cctatcgcac
     2701 gggtattggc aatagaatcg tcaatgtgca acccaaacca ggtgcccatg atgcagaaga
     2761 tggaaaagac gatgtagcag agaaatttat gcggcagacg aaccgtgacc tgcattaacg
     2821 gtatgaataa tggcgtttta ctcattaacc acgcaatgac taaaaaaacg cacatctgct
     2881 gaagcagcag caacaccaga ttaaaatcgt acatacccgc aaaccacact tccctttaaa
     2941 acgcgtaaca tacattgcct gcgtttaact ttctttgaac tcttgcagaa aaatgagaat
     3001 tcgtgagtac gatcactcaa aatcgcctgg caaaaataaa atcaccctat agatgcacaa
     3061 aaaacgggca aaactacctg gttcgcaaaa ctgcgtctaa agttaaaccg ggacctcgcg
     3121 agcaagggtg agacgatggc gctttacaca attggtgaag tggcgttgct ttgtgatatt
     3181 aaccctgtca cgttacgcgc gtggcagagg cgttacgggt tgctgaaacc gcaacggaca
     3241 gacggcggtc atcgactgtt caacgatgcc gatattgacc gtatccgcga gatcaaacgc
     3301 tggatcgaca acggcgtgca ggtcagcaaa gttaaaatgc tgctcagtaa tgaaaatgtt
     3361 gatgtgcaga acggctggcg cgatcagcaa gaaacattac tgacctacct gcaaagcggc
     3421 aatctgcata gcctgcgaac gtggatcaaa gagcgcggtc aggattaccc cgcccagaca
     3481 ctcaccacac atctgtttat tcctctgcgc cgacgacttc aatgccaaca accgactctc
     3541 caggcgctgc tggcgatcct cgacggcgta ctgatcaact acatcgccat ttgtctggct
     3601 tcggcacgta aaaaacaggg taaagatgcg ctggtggttg gctggaatat tcaggatacc
     3661 acccgtctgt ggctggaggg ctggattgcc agtcaacaag gatggcgcat tgatgtcctc
     3721 gcccactcgc tcaatcaact acgccctgaa ctattcgaag gccgtacatt gctggtgtgg
     3781 tgcggtgaaa atcgaacctc cgcccaacag cagcaactca ccagttggca agaacaaggc
     3841 catgatattt taccactcgg catttaatga ttcgttaaca aatgcgcttt actgtacaat
     3901 cctttcgtta acataaggag tgcattatgc gcatagctaa aattggggtc atcgccctgt
     3961 tcctgtttat ggcgttcggc ggaattggtg gcgtcatgct cgcaggttat acctttattt
     4021 tgcgtgctgg ctaagcgcct gcaccagcct ttcaaacagg cggtctgcga tgatcgccgc
     4081 cagtgccacc agtaacgccc cctggatcac atacgcggta ttaaatccgc taagcccgat
     4141 gatgatgggc gtacccagcg tgctggcccc taccgttgag gcgatcgtcg ccgtaccaat
     4201 gttgataatc accgaagttc gcacgcccgc cagaatcacc ggagccgcca gcggtagctc
     4261 gaccttacgc agtcgctgac cacgactcat tcccatacct ttcgcaactt ctgtcacgct
     4321 ggcatcgatc gctcccagcc cggcaagtgt cgcctgcagg acgggcagca caccgtaaag
     4381 gatcaaggcg ataatcgctg gttgcagacc aaagccgatc accggaacgg caatcgccag
     4441 cactgcgaca ggcggaaaag tctgtccaac ggcggcaata gtttccacca gtgggcgaaa
     4501 ttccgcgccc cacgggcgag tgacagcaat tccggcacca gtgccaatga tcaccgcaaa
     4561 caaactcgaa attcccacca gccagaaatg agccagtgcc agagctgcaa aactttcttg
     4621 ctgataaacg ggtcgtggca gttgtgggaa caaggcagca aacagcggct ggctgtaagg
     4681 cagccagaaa atcagtgcca caaacaaagc aatgagccag aacagcggat cgcgcaacat
     4741 cttcatacgc ttacgcctcc accagcagat cctgaaaatg cagcgtaccg caaggctggc
     4801 cctgcgtgtt caccaccggc agcacctcgc aaccccgcgc gacaaacagg gagagcgcat
     4861 cgcgtagcgt catctcttct accagtgcct caccttctgc ccgttcttcg cgacgcacgt
     4921 aatccgccac actacgtaac gaaagcagac gcacacccag ttcgctacgt ccaaaaaact
     4981 ggcggacaaa atcattcgcc ggacgagtca gcatcgtcag cggattgccc tgctgcacca
     5041 cttcaccgtg atccatcaat accagatgtt cagccagccg tagcgcctca tcaatatcat
     5101 gagtgaccag cacaatagtg cgccccagca aacggtgaat gcgcgtcatc tcttgttgca
     5161 acgcgccgcg cgttaccggg tccagtgcgc caaaaggttc atccatcagt aagacttgcg
     5221 gatcggcagc cagtgcgcgc gccactccca cacgttgctg ctgaccaccg gaaagctgat
     5281 gcggataacg ctcacgcaaa tttgactcca gccccagtag cgtcattaat tcgtcgatac
     5341 gatcgtcaat ccgtgcccgc gaccattttt gtaattgcgg cacggtagcg atgttttgcg
     5401 ccacgctcca gtgggggaac aggccaatag attgaatggc atagcccatc cggcggcgca
     5461 actccagtac tggcagcgag cgaatttctt ctccggcaaa gcggatctct ccgctgtcat
     5521 gttccaccag gcggttaatc attttcaagg tggtggattt gccggagcca gatgtgccaa
     5581 tcagcaccga aaaactccct tcctgaaaat tgagattaag atcgttaacg gctttttgtg
     5641 cgccaaacag tttgctgaca tggctaaatt caatcattac gtttcacctt cagcagtgcg
     5701 ataagcaaat cgaacagggc atcgatcagc accgccagaa caattaccgg gatcaccccc
     5761 agcaacacta aatcaatggc gctgcttagc agcccctgga aaaccagcgc accaaaaccg
     5821 cctgcgccga ttaacgccgc aatcaccgcc atacctacag tttgcaccat caccacccgc
     5881 aggctgcgca gaaataccgg taacgccagt ggtaactgaa catgcaggaa tcgctgcgcc
     5941 ccgctcatgc ccatcgctct ggcgctctcc agcacatcgc gcgggatctg gttcaaaccg
     6001 actaccacgc cgcgcaccag cggcagcaag gcatagagca ccagcgcaat cagtgcgggt
     6061 gtcattccgg ttcctgctat gccgagcttc cccagccacg gaaaggccgt caccagcgcg
     6121 gcaagcggcg caatcaacag gccaaagagc gccaccgaag gcacggtctg aatgacattg
     6181 agcagagaaa aaattgcccc ctgccgcgca gtggaaaagt agcaccagat gcccaacggc
     6241 acaccaatca ctaacgcagg cagcaccgca ccaaacagca acgtcagatg ttgtgccagc
     6301 gcgtcgtcaa acacatcctg acggttggcg tattctttca ttagtgagag atcgttaagc
     6361 gtgccggagt acagcaacca cagcggaata atggcaatct gcatatgcaa caaccagcgc
     6421 cacagcggat gcgtggagat tcggcggatg gcatcgctac aggccagcaa tgccagcgcc
     6481 gcagccagcc agaaaccact gccgaggctg gtacgcgcca gcgcactgcc attttgcgcc
     6541 agttgggtcg ccgcctttcc agctccccac accaacaata cgaagacgaa ttgcgccaga
     6601 atgagtgcac aaatgctgcc ttttttagcg gggacaaaac aggctgtcag ccaggcgcaa
     6661 ccaacgccca ccagcatcca gatcgtttgc ggccacagtt gccagagatg acgcccttca
     6721 cccgagacta aacgattagg cgcgtagctg ataaacggca gcgctgctgc gattgccgtc
     6781 agcaacagca gcagcgccag aacaggatta atacgtagat aagtcacggg aaattacttc
     6841 gcccaccctt tttgtttcag gtagtcggca gccacttttt tggcatccag tccttccact
     6901 gcaatgctgg cattcagttg ctgcaatgtt tttgcatcga ggctggcgaa gactggctgt
     6961 agccactgtg ccatttgcgg atactccttc aacaccgatt cacgcaccac tggtgcaggc
     7021 gcatagatag gttgcacgcc ttgcggatcg cttaaggttt gcagccccag cgccgctacc
     7081 gggccgtcag tgccgtaagc cattgcagca ttaacgccag aggtttgctg ggcagcggct
     7141 ttgatcgtca ccgccgtgtc gccaccagcc agtgacagca actgatcctg accgagctta
     7201 aagccatagg ctttttcaaa cgcgggtaac gcatcggcgc gttcgataaa ctctgccgag
     7261 gctgccagtt tgaaggtgcc gccctcttgc agataacgac tcaggtcggc aagcgaagtg
     7321 agtttatttt tctctgccac atcctgacgc acggcgatag tccaggtgtt atttgcaggc
     7381 gcgggcgtca gccagattaa cttgttgtgc tccgaatcga gctttttgac tttctcgtaa
     7441 ccttgctgcg cgtttttcca cgctgcatcg ttttcatctt taaagaagaa agcgccattg
     7501 ccggtatatt ccggatagat atccagttca ccggaagtaa tcgccccgcg caccacagga
     7561 gtcgttccga gttgcacttt attcaccgtc ggtacgccgt ggctttccag cacctgcaaa
     7621 atgatattgc cgagtagcgc accttcggta tcgatttttg aaccgacttt aacgggggaa
     7681 gccgcctgta gcggcaggct cacggctgcc aacataacca gtgaacctgc ccagagcttt
     7741 aagagtggca tgatgctttc ctcattcttt tactgttgtt ttcagcgaat taagagaaaa
     7801 gcatagttga taatggcggg gttagcttga gcggaaggat tcagttgcag aatcagataa
     7861 atacttaaga ggcatattcg gtccggcgtt tcctgccgga tgcggcgcga gcgccttatc
     7921 cggcctacaa agggcgcaaa cgtcgcgccc ttactaaagc attacagcaa ctcaaactcg
     7981 cctttcttaa cgcgtgcgga atcagtgccg ataaatacat tgaacttgcc aggctcggcg
     8041 tcatatttca tctgttgatt ccagaacttc agcgcctcaa tatcgatcgg gaagctgaca
     8101 gtctgagttt cgcccggttt cagggtgatt ttctcaaagc ctttcagctg tttcacaggg
     8161 cgactcatgg aagccgtcac atcctgcaag tacatctgca ctaccgtggc cccctcgcgc
     8221 ttaccggtgt tcgtcacctg cacgctggca gtcactttgc cgtcacgctt catggtcggc
     8281 gcagaaagtt tcacatcaga gacggtgaaa gtggtgtagc tcagcccata gccgaacgga
     8341 tacaacgccc cgttagcttc atcaaaataa cgcgaagtgt atttgttcgg cttgtcggca
     8401 ttatacgggc gaccggtatt cagatggctg tagtacaccg ggatctgccc gacagaacgc
     8461 gggaaggaca ttggcagctt gccggacggg ttgtaatcgc caaacaatac atcggcaatt
     8521 gcattaccgc cttcagtccc cgcaaaccag gtttccagaa tcgcatcagc ctgctgatct
     8581 tctttcacca gcgccagcgg acgcccgttc atcagcacca gcaccagcgg tttaccggtg
     8641 gctttcagcg ccgcaatcaa gtcacgttgg ctttgcggaa tagtgatatc ggtccggctg
     8701 gaggcttcgt gcgccatccc ctgtgcttca ccgactacag ccaccaccac atcagattgt
     8761 ttcgccgtct gcaccgcttc atcaatcatc tcttgcggcg aacgcggatc gactttgacc
     8821 gcttcttcat actgattcag gaaatcgata atgcctttgt cactggtaac gttcgcccct
     8881 ttggcataca gcactttacc gttttcaccg acggaatttt taatcccggt cagtacggtc
     8941 acggattgat cggcaacacc ggctgcggac cagctgccca tcacgtcacg tttactgtcc
     9001 gccagtggcc caaccaccgc aatggtggcc gattttttca gcggtaacgt ttcgagacgg
     9061 tttttcagca acaccaggct ttcgcgcgcc acttcacgcg cttctttacg gtgcaggcgg
     9121 ctttcggcat tggtatccac cgggtcagac tctttcggcc ccaaatggct gtatgggtcg
     9181 ttaaacaatc ccatatcata tttaacgttc agtacatggc gggcagcatc gtccagctct
     9241 tccatcgtca ctttgccgga gttgatcaac ccaggcagat acttcgagta atactcgtcg
     9301 ctcatactca tgttgattcc ggatttcagc gccacgcgca ccgcatcttc cgggtctgcc
     9361 gccgtgccat gtttaatcag ctctttgatt gcaccgtgat cggaaacggt gatgccttta
     9421 aagccccact ggtcgcgcag aacatctttc agcagccagg aatcggaggt ggctggcgtg
     9481 ccgttcagcg agttcagcgc caccatcacc gcgccgctgc ctgcgtccag ccccgctttg
     9541 tacggcggca tataatcatt aaacaggcgc tgcggactca tatcgacggt gttgtactct
     9601 ttaccgcctt ctaccgcgcc gtatgcggca aagtgtttga cgctggtcat caccgagtag
     9661 cgatctgccg ggcttttacc ctgcatcgct tccaccatgg ttttacccat tgttgaggtg
     9721 agatacgtat cttcgccaaa accttcggaa gcacgtcccc agcgcggatc gcgcgagaca
     9781 tcgaccatcg gtgcccaggt catattcagg ccatcatctg ccgcttcata agcagagaca
     9841 cgtccgaccg ttttcactgc atcgaggtta aaagacgagg ccagaccgag gctaatcggg
     9901 aacaccgtgc gctgaccgtg cagcacgtcg taagcaaaga aaagaggaat tttcaggcgg
     9961 cttaattcca tcacctgatc ctgcatggcg cggatatcct gacgggttac ggtgttgaaa
    10021 atcgccccaa cctgaccgtc tttgatcatc tcgcggatcg cctctttcgg gttatccggg
    10081 ccgacgctga ttaagcgcag ctgaccaatt ttctcatcaa ctgtcatttt cttaagcagt
    10141 tcggtgacga acgcatcccg cgcttcgggc gttaatggat ggttgccgaa taaatcatcc
    10201 gccagtgctg gctgcagggc cagactcacc gcgattccta ctgaacatag ccatttcata
    10261 tggatttttc ttcctctgtt gcccgacgag gcagcgaaaa tggtgcaaaa accgtagttt
    10321 gccataagca tgatggagag agaaaaagaa tgctcagttt attgtctgaa ttttcaaaat
    10381 attcactcgc tgaattgtca tacaaggcgc tattctagtt tgtgatattt tttcgccacc
    10441 acaaggagtg gaaaatgtct tccatgacaa caactgataa taaagccttt ttgaatgaac
    10501 ttgcccgtct ggtcggtcat tcacacctgc tcaccgatcc cgcaaaaacg gcccgctatc
    10561 gcaagggctt ccgttctggt cagggcgacg cgctggctgt cgttttccct ggctcactac
    10621 tggaattgtg gcgggtgctg aaagcctgtg tcaccgccga caaaattatt ctgatgcagg
    10681 ctgccaatac aggcctgacc gaaggatcga cgccaaacgg taacgactat gatcgcgata
    10741 tcgtgatcat cagcaccctg cgtctcgaca agctgcacgt tcttggcaag ggcgaacagg
    10801 tgctggccta tccgggcacc acgctctatt cgctggaaaa agccctcaaa ccgctgggac
    10861 gcgaaccgca ctcggtgatt ggatcgtcgt gtataggcgc atcggttatc ggcggtattt
    10921 gtaacaactc cggcggctcg ctggtgcaac gtggcccggc gtataccgaa atgtcgttat
    10981 tcgcgcgtat aaatgaagac ggcaaactga cgctggtgaa ccatctgggg attgatctgg
    11041 gcgaaacgcc ggagcagatc cttagcaagc tggatgatga tcgcatcaaa gatgacgatg
    11101 tgcgtcacga tggtcgtcac gcccacgatt atgactatgt ccaccgcgtt cgtgatattg
    11161 aagccgacac gcccgcacgt tataacgccg atccggatcg gttatttgaa tcttctggtt
    11221 gcgccgggaa gctggccgtc tttgcggtac gtctagatac cttcgaagcg gaaaaaaatc
    11281 agcaggtgtt ttatatcggc accaaccagc cggaagtgct gaccgaaatc cgccgtcata
    11341 ttctggctaa cttcgaaaat ctgccggttg ccggggaata tatgcaccgg gatatctacg
    11401 atattgcgga aaaatacggc aaagacacct tcctgatgat tgataagtta ggcaccgaca
    11461 agatgccgtt cttctttaat ctcaagggac gcaccgatgc gatgctggag aaagtgaaat
    11521 tcttccgtcc gcattttact gaccgtgcga tgcaaaaatt cggtcacctg ttccccagcc
    11581 atttaccgcc gcgcatgaaa aactggcgcg ataaatacga gcatcatctg ctgttaaaaa
    11641 tggcgggcga tggcgtgggc gaagccaaat cgtggttggt ggattatttc aaacaggccg
    11701 aaggcgattt ctttgtctgt acgccggagg aaggcagcaa agcgttttta caccgtttcg
    11761 ccgctgcggg cgcagcaatt cgttatcagg cggtgcattc cgatgaagtc gaagacattc
    11821 tggcgttgga tatcgctctg cggcgtaacg acaccgagtg gtatgagcat ttaccgccgg
    11881 agatcgacag ccagctggtg cacaagctct attacggcca ttttatgtgc tatgtcttcc
    11941 atcaggatta catagtgaaa aaaggcgtgg atgtgcatgc gttaaaagaa cagatgctgg
    12001 aactgctaca gcagcgcggc gcgcaatacc ctgccgagca taacgtcggt catttgtata
    12061 aagcaccgga gacgttgcag aagttctatc gcgagaacga tccgaccaac agcatgaatc
    12121 cggggatcgg taaaaccagt aaacggaaaa actggcagga agtggagtaa aaattacgga
    12181 tggcagagta tcgccatccg aattcactta atcgttctgt gccgtctgcc ccgccgccgc
    12241 catttgggcg gctttttgtt ttttatagct caacgctgct gccggcacag gcatcacttt
    12301 accggtttca atccaggtac gcaggcggct ggcatcggca aaatgggtat atttgccaaa
    12361 cgcgtccatc actaccagcg ccaccggttt attattgata accgtacgca tcaccagaca
    12421 atggcccgcc gcattggtaa agccggtttt ggttaactga atattccagt tatcgcgata
    12481 caccagatga ttagtattgc ggaacggcag cgtatacgtc gggttagaga aggttgccat
    12541 atcttcccgg gtagtactta actgcccgat caacggatat tgtttgctgg caatgagcag
    12601 tttggttaag tcacgggcag ttgaaacgtt atgcaccgac aatccggtag gttcaacaaa
    12661 gcgcgtgttg ttcattccga gcgatttcgc tttcgcattc attgccttaa taaaggcttt
    12721 gtaaccaccg ggataatggt gcgcaaggct tgccgccgcg cggttttctg aagacatcag
    12781 cgccagcaac agcatatctt tacggctgat ttcgctattc agtcgtacgc gcgaatagac
    12841 ccctttcatc tccggcgtct ggctgatatc cacttttagt ttttcatcca gcggcagtcg
    12901 tgcatccagc acaaccatcg cggtcattaa tttgctgata gacgcaattg gacgcaccag
    12961 atccgggtgg ttcgaataga tcactttgtt ggtattcaga tcaacaatca tcgcgctacc
    13021 ggaggcaatt tccggttgtg aagcggcggt agcggctgcc gttttcgcaa cggcctgcgg
    13081 tgccaaaggc acagccagca tcagggccag gctaaataaa gaaactcgaa atttcggcat
    13141 gatgagcatt cagatagtgg ttcacgcgca cgggttgcgc accgccggag taaggattta
    13201 ctgaggctag cgacgccatc ataacgagca aaaagtgcga tcgtcaaagg agaatcgtga
    13261 ggaaatgctg cattgctgac atttacgcca gcaatgcaat gtcaaagaac tttctagaac
    13321 aggcgatacc cgtagcccca tagtataacg gttagggcga gcagcacttc cagtaccagc
    13381 acgccaatcg ccagcgtcga actggagaag ctaaggcctt cctctttgtt gatattcagg
    13441 aagctcggaa taccaaggta aagcaggtag ccggtgtaaa acagcgccac cgtgccgacc
    13501 agcgcacaca accagaccag cggataaagc gccaccagac cgcttaaaaa cagcggagtt
    13561 gcaacgtagc ccgcgaagac catgcagtgc gcaagtgacg gacgctgcgg gtaattgcgc
    13621 gccatccacc agatgacccg ccccatcacc gcgaccccag ccagcataac gccgtaaaac
    13681 aagacagcca gcgccagtcc ggtaaaccag gataacttca ggatagtgcc atcgccaaaa
    13741 ttccagccaa tctgtgtagt gccaatgaag gcgcaaatca ccggaatcgc cgccatcagc
    13801 aaaacgtggt gggtgtaatg gtgagaaatc gtttcgtttt cgcgattaat cacctgcatt
    13861 tcacgatcgg gatgggaaaa cagtccccag acatggctca taccgccccc ttgttgtgag
    13921 ttcatgaacc tgacagttca agtataagtc agcttgtgat tattttttgt tcgcccatgt
    13981 aattttcact gtctgatgaa cgtccttttt cagcgtgaat gattcacagg gtgtatgctt
    14041 actggcaacc aaagggagac agactggcct atggatctca atacacttat ctcacaatat
    14101 ggttatgccg cgctggtgat cggtagcctg gcggaaggtg aaaccgtgac tttgctggga
    14161 ggcgttgcgg cacatcaggg gctattaaag ttcccgctgg tggtactttc tgtggcgttt
    14221 ggcggcatga ttggcgacca ggtgctttat ctgtgcgggc ggcggtttgg cggcaagctg
    14281 ttacgccgtt tctcgaaaca tcaggataaa attgagcggg cgcagaaact tatccaacgc
    14341 catccgtatc tgtttgtcat tggtacgcgc tttatgtatg gctttcgggt gattggcccg
    14401 acgctgattg gtgccagcca gctgccgccg aaaatctttc tgccgctgaa tattctcggc
    14461 gcatttgcct gggcgttgat ttttaccact attggttacg ctggtggtca ggtgattgcg
    14521 ccgtggttgc acaatctcga ccagcatttg aagcactggg tctggttgat tctggtcgtg
    14581 gttctggtgg tgggcgtgcg ctggtggctg aaacggcgcg ggaagaaaaa gccggatcat
    14641 caggcgtaaa accattgccc tgggtaaggc gttcgcgccg catccgacat ccggtgcacc
    14701 atgcctgatg cgacgcttgt gcgtcttatc atgcctacca aaattttacc cattgaaata
    14761 accacgcacc tcactgcgct attctgggtt gaactgcgga ttcgccaaca taaagccgcc
    14821 atccactatc aacgactgcc cggtggtgta atttgcgccc tctgagcaca gccacgctac
    14881 caggctggca atctcatgcg ttgcgccaaa acgccgcaga ggaatcgaag gctccgcgtc
    14941 gggcttcacg tcgctgtcat ccatgccatt cattggcgtg gcgatcgccc caggcgcgac
    15001 tgcgttcacc aaaatcttat gcctgaccag ctccagcgcc atcgttttgg ttaacccgcc
    15061 gagcgcatgt ttagcggctg tgtaagcgct ggcatccggc agcggcgtat gttcatgtac
    15121 cgacgtaatg ttgatgatgc gaccgccctg cccttgtttc accatctggc gggccgcaat
    15181 ttgtgagcat aaaaatgcac catcgacatc aacggtaaaa atcttgcgcc actcatcgaa
    15241 agccatatca agaaacggcg ctttggtcat tgcgcccgca ttattcacca gcacatcaat
    15301 acgccccagc cgttgaatga gtttctccag cgcctgcgca ccttctggca gtttaccgag
    15361 atccagctgc acgatctccg cacgtacgcc gtggctaact acctcacgcg cggtatcttt
    15421 tgccccttct tcatctgagt gccaggtaat gccaatatca aacccctgct gcgccagtaa
    15481 taacgcgcac tctttgccga tccccgaatc ggaggcggta ataatcgcaa cctgtgccat
    15541 cgagttctcc acttaacgct gaataaacgt taagtataga aggcgcatat catcagcgtt
    15601 tgtaaccccc gcccaacgca ccagtgagtt gaatggaagc atccagccac tgcccttgca
    15661 ataacaggcc attggcccgc tcacgcagcg cggggattct ggcttcgctg acgcgggaac
    15721 cagcaatgat gcccgcgtta aagcgcgcct gcgcaagacc taccacacgc aaggcatcgc
    15781 gctcaatttg cgcctgatgc tggtttttct ccgcaagtgt ctgaacctga ctggctgccc
    15841 gcgccacgtc attcaccgct tcaaccaccg ctttgttgta gctggcgata gacaagttgc
    15901 ttccggcttt tgcgatatcg agattggcgt taagacgacc actatcgaaa atgggtagcg
    15961 tcaggcctgc cgtaacgccc atttgctgcg cggaatgacg gaacagatcg cttaagtgca
    16021 acgcatcctg ttgcaggaag gccatcaggt tgatgtcagg ataaaatgcc gcttttgccg
    16081 catcaatggt gcttagcgat gactcaacgt accagtgcgc cgcctgcaaa tctgcccgcc
    16141 gggccagtaa ggagtacccc agttcatcag gaagctggct tgccactttc ggcaacgcga
    16201 ccggtttaag cttcaatgac tttgtctggt tatttgtaag tgcgcttaac cgtgcctcaa
    16261 taattttcat tttccccgcg acatcgttga gctgctgccg ggttttgctg gcattaatat
    16321 cggtttccac accgtcaact gaagaagtaa tcccgttctg atatagctgg cgatcggtcg
    16381 cgataatggt gttctgctct ttttctattt gctgcaagac cgtgtttaac gccgcctggg
    16441 tttgccactc ccagtacagg cgggctacgc tgccagccag caattggcgg gtttgctcgc
    16501 gttccgccgc ccgtgcttta accgtaccca ggcgggcagt aacctccgcc cgattctttc
    16561 cccagatatc gagatgccag cccgccgtta agccaaaagt tccgttggtg taccacggac
    16621 cggtcgtacc tgcggccgga tcgttcagag caaacggccc cattaagcct tctgccgaca
    16681 ttttttgccg ctccatatcc gcagaaaagt cgatctgcgg accatcctga gtagcaattg
    16741 ccttcgcctg ggcttcagct agctgaatgc gctgttcagc cacctgcata tccggtgcgt
    16801 tctgtagtgc attgttaatt aaggaagtga gttgattatc gtgatactcc agccaccatt
    16861 ggctgtctgg ccaaccattt ttcagcgccg tgggtaatgc ggtgtcaact tgtgcagcgg
    16921 gcgtttgctg gcttaacgcc tggcgggttt catgcatagg cgcacacccg gccagcatca
    16981 gtaacagcgg aaaacaggcg atggcgggat aaaaggaatc acgattcatg ggggaataat
    17041 caggtaagaa aaggtgcgcg gagattaccg tgtgttgcga tatatttttt agtttcgcgt
    17101 ggcaatacat cagtagcaat aaaacgacat atccagaaaa atatacacta agtgaatgat
    17161 atcttccgat ttatcttaat cgtttatgga taacggcaaa gggcttcgtt ttttcctata
    17221 cttattcagc actcacaaat aaaggaacgc caatgaaaat tatactctgg gctgtattga
    17281 ttattttcct gattgggcta ctggtggtga ctggcgtatt taagatgata ttttaaaaat
    17341 cagatatggc atataagttg tcaggtcagg aactagatat tttgtgtact tgatttgcaa
    17401 acagctccgg tgttggtggc gtcaaaatcg ctgaagcgtt ccccgaaggc cggggcagcc
    17461 cacatggatg tgggctgagg gcgcgtctta caaggacgtt tcctcgcgcc cgacccggta
    17521 gccgaagg
//
LOCUS       DAZFXT010000096        17438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.096, whole genome shotgun sequence.
ACCESSION   DAZFXT010000096 DAZFXT010000000
VERSION     DAZFXT010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.096"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(57..1415)
                     /gene="plaP"
                     /locus_tag="ACHKW0_003468"
     CDS             complement(57..1415)
                     /gene="plaP"
                     /locus_tag="ACHKW0_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HFO7476245.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(1405..1467)
                     /gene="yoeI"
                     /locus_tag="ACHKW0_003469"
     CDS             complement(1405..1467)
                     /gene="yoeI"
                     /locus_tag="ACHKW0_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HFO7476246.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(1682..2611)
                     /gene="yeeY"
                     /locus_tag="ACHKW0_003470"
     CDS             complement(1682..2611)
                     /gene="yeeY"
                     /locus_tag="ACHKW0_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HFO7476247.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(2657..3481)
                     /gene="yeeZ"
                     /locus_tag="ACHKW0_003471"
     CDS             complement(2657..3481)
                     /gene="yeeZ"
                     /locus_tag="ACHKW0_003471"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HFO7476248.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(3564..3818)
                     /gene="yoeB"
                     /locus_tag="ACHKW0_003472"
     CDS             complement(3564..3818)
                     /gene="yoeB"
                     /locus_tag="ACHKW0_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HFO7476249.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(3815..4066)
                     /gene="yefM"
                     /locus_tag="ACHKW0_003473"
     CDS             complement(3815..4066)
                     /gene="yefM"
                     /locus_tag="ACHKW0_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HFO7476250.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            4349..4399
                     /gene="hisL"
                     /locus_tag="ACHKW0_003474"
     CDS             4349..4399
                     /gene="hisL"
                     /locus_tag="ACHKW0_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HFO7476251.1"
                     /translation="MIRVQFKHHHHHHHPD"
     misc_feature    4375..4498
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            4545..5444
                     /gene="hisG"
                     /locus_tag="ACHKW0_003475"
     CDS             4545..5444
                     /gene="hisG"
                     /locus_tag="ACHKW0_003475"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HFO7476252.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            5450..6754
                     /gene="hisD"
                     /locus_tag="ACHKW0_003476"
     CDS             5450..6754
                     /gene="hisD"
                     /locus_tag="ACHKW0_003476"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HFO7476253.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNVETFHTAQ
                     KLPPIDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVQDVFNIGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDTD
                     MAHQVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            6751..7821
                     /gene="hisC"
                     /locus_tag="ACHKW0_003477"
     CDS             6751..7821
                     /gene="hisC"
                     /locus_tag="ACHKW0_003477"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HFO7476254.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDS
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            7821..8888
                     /gene="hisB"
                     /locus_tag="ACHKW0_003478"
     CDS             7821..8888
                     /gene="hisB"
                     /locus_tag="ACHKW0_003478"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HFO7476255.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            8888..9478
                     /gene="hisH"
                     /locus_tag="ACHKW0_003479"
     CDS             8888..9478
                     /gene="hisH"
                     /locus_tag="ACHKW0_003479"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HFO7476256.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            9478..10215
                     /gene="hisA"
                     /locus_tag="ACHKW0_003480"
     CDS             9478..10215
                     /gene="hisA"
                     /locus_tag="ACHKW0_003480"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HFO7476257.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNV"
     gene            10197..10973
                     /gene="hisF"
                     /locus_tag="ACHKW0_003481"
     CDS             10197..10973
                     /gene="hisF"
                     /locus_tag="ACHKW0_003481"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HFO7476258.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            10967..11578
                     /gene="hisIE"
                     /locus_tag="ACHKW0_003482"
     CDS             10967..11578
                     /gene="hisIE"
                     /locus_tag="ACHKW0_003482"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HFO7476259.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(11674..12651)
                     /gene="wzzB"
                     /locus_tag="ACHKW0_003483"
     CDS             complement(11674..12651)
                     /gene="wzzB"
                     /locus_tag="ACHKW0_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HFO7476260.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIVTQPDVGQIAGYTNAMNVIYGSAVPKVSDIQASLIGRYSTA
                     FSALAETLDNQEEAEKLTIEPTVKNQSLPLAVSYVGETPEGAQKQLAKYIQQVDDQVN
                     EELGKDLKDNIAQRMKNLQDSLKTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIESLKVDDLDIHA
                     YLYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(12798..13964)
                     /gene="ugd"
                     /locus_tag="ACHKW0_003484"
     CDS             complement(12798..13964)
                     /gene="ugd"
                     /locus_tag="ACHKW0_003484"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HFO7476261.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRYADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(14212..15618)
                     /gene="gndA"
                     /locus_tag="ACHKW0_003485"
     CDS             complement(14212..15618)
                     /gene="gndA"
                     /locus_tag="ACHKW0_003485"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HFO7476262.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(15724..16752)
                     /gene="galE"
                     /locus_tag="ACHKW0_003486"
     CDS             complement(15724..16752)
                     /gene="galE"
                     /locus_tag="ACHKW0_003486"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HFO7476263.1"
                     /translation="MAILVTGGAGYIGSHTVLMLLKEQYEVIVLDNFHNSSVESLHRV
                     KEITGSDICIINGDIRDRVILKSIFTENSITDVIHFAGLKSVSDSIQFPTEYYDNNVY
                     GTLVLIEEMISNNIKNLIFSSSATVYGTPEKIPVKETFPVGGTTNPYGTSKLMVENIL
                     HDVSLAYPFFRTTILRYFNPVGAHPSGKIGEDPNGIPNNLMPYICSVASGKYKQLSIY
                     GNDYETKDGTGVRDFIHVMDLANGHIAALKHRDEGSNYKVYNLGTGHGYSVLELLAAF
                     QRITSVNIPYVLVERRAGDIAECWSDPSKAYLELGWKACLGLDEMVRDAWNWQQSNPD
                     GYKFSEKN"
     gene            complement(16831..>17438)
                     /locus_tag="ACHKW0_003487"
     CDS             complement(16831..>17438)
                     /locus_tag="ACHKW0_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7476264.1"
                     /translation="KDYKVIGGIGVNLNEFSYHPPICGEIHFGMVARLLREKGVCEFV
                     EAAKIIKKKYPMVRFSIAGDFDDNPGGIDAAQIVNWKAEGYVEFLGPCSYIKEYLSDI
                     SVFVLPSYREGVPKSTQEAMAIGRAIITTDVPGCRETVIDGYNGYLVPPWDTQKLALV
                     METFIHERELIVTMGLNSRRFALSKFNESESANSLVNIITA"
BASE COUNT         4546 a         4394 c         4217 g         4281 t
ORIGIN      
        1 taaaaaaacc ggagccaaat attcattcga ctccggttta ttattacaaa agatgattac
       61 gctacgtctt cgtactgcgg aaccgggtta cggaaactct tggtgacgca agccaggtaa
      121 atcagaccga tagccgccca gatcagaccc agaaccattg agctttcttc caggttaacc
      181 cacagcgcac caacggtcag cgcaccacac atcggcagga acagatactg gaagtgatct
      241 ttcagcgtct tattacgctt ctcacggatc cagaactgcg agatgaccga caggttaacg
      301 aaggtgaacg ccaccaacgc accaaagtta atcagcgctg tagccattac caggtcgaag
      361 ttgattgcca gcagggcaat cgcgccaacc aggatgatgt tcatcgccgg agtacgccat
      421 ttcgggtgta cataaccgaa gaagcttttc gggaatacgc cgtcacgacc cattacgtac
      481 atcagacgcg ctacgcctgc atgcgccgcc ataccggacg ccagtacggt aatggtggag
      541 aagatcagcg cgccaacctg gaacgcttta cccgcaacgt acagcatgat ttcaggctgt
      601 gacgcatccg gatctttaaa gcgagagata tccgggaagt acagctgcag gaagtaagtt
      661 gcaaagatga agatcatgcc gccaatcagc gcggtcagga aaatcgcacg cgggatcacg
      721 cgctctgcat ctttggtttc ttccgacagg ttgctgatgc cgtcaaagcc ggtaaaggag
      781 aagcacagga ttgtcgcccc ggtaatcatc gggataacat gcgcatcgcc agaccagaac
      841 ggacgagtgc tcgccagcgt accagcgcct tcaccttcaa atacgccata aacaaccatg
      901 cccagaatca ccgcgatcag caccacctgc aacacgacga ttacagtgtt gaagttcgct
      961 acggatttca gactacgcag gttaaaggcg gtcatgaagg ccaccaacgc caccacaaac
     1021 atccacgatg ggatggaagg caccagagct tcaaaataga ttttcgccag cagaatgttg
     1081 atcatcggcg cgaacagata gtcgagcaga gaagaccaac ccaccataaa gccgacagtc
     1141 gggctaatgg atttctgggc gtaagtgtat gcagagccag cagaaggata gcggcgaacc
     1201 agcttcccgt agctcagagc cgtaaacagg atcgcaatca atgcgaacgc ataggctgtc
     1261 ggcacatgac catccgtgag gcctgaaacg ataccaaaag tatcaaacag cgtcatcggc
     1321 tgcatatagg caagacccat cattacaacc ggaactaacg taagcgtttt acgcaattcc
     1381 acgcgagagg tgtttggagt aacgttatgc gacatggtca ttctccttta cggtgataac
     1441 cgtcgcgtaa gcaaaaaatt gccccatttt tttggattcc tcagcgacaa caactgtcga
     1501 tttttagtaa atatctatcc ggtacgaagc ccggcctctt ggtatgaatg attggtttga
     1561 agcaaaaaat aaccgacgct gatgaaacgt cggtttttag tcattttttg acagcggcgc
     1621 attgtgccta aatggggggg aaatgacaag agaatgagag gcttgtcata ataatttttc
     1681 tttaaatggc tgattttccg tcatcagatt ttgcataaac accggcgatc gcactatttg
     1741 ctaaaatttc atcccgccac cagccactgg caagtcctgc ggtctgttca ttccagccaa
     1801 tccagacggg ttcaaagaga gtttgggcga ccactttctt ctcgattaac gcaccactat
     1861 cgagaaaacg ttgcgccaga taacggggta aatagccgca gccgaggccg ctaatttgca
     1921 gctccagctt ggttttaaaa tcgaaaacgg taatggcttc ttgttcgtcg agaagttgtg
     1981 aggccgtaga agcgcctgcc tgcgcagtat cccccaccac aatcgcccga tatcgcttaa
     2041 tgatacgacg gtttaacggt tcttcttcca gggctaacgg atgatgaggc gctaccgcaa
     2101 agacttgttc cagatcgccc aaccgcgaga aaccaaactc actggaagat ggtggctcat
     2161 gcatcgcgcc aacaataata tccgctcgcc cctgagtcag ggcatcccat gaaccaccga
     2221 gtacgccatt gataaacttc aggcgcgtca cgctatgatg ttgataaaaa gcttcaatca
     2281 gtggcgcaag cagagaaaaa ggaaacgtgt cgtccacgcc aatcaccagt tcgttttccc
     2341 agccttcatg cagtttgatc gcctgctttt ccagttctcg cacggtatgc agaacttccc
     2401 gccctttctc taataacatt tttccggtgc gggtgaattt agcgcggtgt ccgctacgat
     2461 caagcaattg aatattaagg tcgctttcca gcttgtgaac ggtgtaactc agagcggacg
     2521 gcgtcttata gagtttggcc gacgccgccg caaaactgcc ttctttttct aaggcatcga
     2581 ggatcatcaa cacatccagc agtggtttca tacacgcccc cttgcagtgc atgcggcgtc
     2641 ctgctggcgt gatcgtttac tccagcggca ttaccagcgg atcgggatac tggtattcaa
     2701 atcccagttc attacaaatc cgactgccat caataatctt acctttgccg ctgtccagac
     2761 tatttctgaa ctgcggaggc tccagcccca gtaaacgggc catctgcgga tagaaaacat
     2821 tacgcgcagg gtgagctggc gcacatatat tatagatgtg tccgccttta ggtgcctgta
     2881 acaacagagt gatagcgcca ataacatctt ctaaatggac taaattaaca ccatgttcac
     2941 catcaggcgc ggtttttccg gcaaagaagc gtccgggatg acgtcccggt ccgaccagcc
     3001 ccgcaagacg cagaatatcg accgaagtac cgggtaaatt gtgcagccag tcttcgagtt
     3061 cttctaatac tcgtccactg ttggtcaccg gattacgcgg ggtggtttct ttcaccgtgc
     3121 cttgcgcgtc gccatagaca gacgttgagc tggtaaaaat aattcgggga atacgatggg
     3181 ccagcgcgct atccactaac tcttgtaccg cttgtaaata gaactcatcg ccggggccgc
     3241 tacgacgtgc cggaagcgta atgaccagcg catcggcatc catcagggca tccagatcgt
     3301 cagaatcgca aactaactca ggctccatgc gaagcagata gctgtcaatg ccactcattc
     3361 gggccgcttc gacaccatct tgtgtggttt tactcccggt gacttgccag cctcgcgctg
     3421 aaagtgacat cgccagcggc atgcctaacc accctaaccc gacaattgcg acctttttca
     3481 tccgttatct cctgatacac cttagatcta taaggctacg ctagcgtatc aaaactgaca
     3541 attcattcta tgaatgaatc tgttcaataa tgataacgac atgctgcaat gagcagtgaa
     3601 tcgtcggtaa ccgcgtatac cagacggtgc tcctctgtaa tgcgtcggga ccagaaacct
     3661 gacaaattat gtttcagggg ttctggcttc cccttacctt caaatggcgt tctgcgggta
     3721 tctttgataa gttcattgat ctttttaaca attcgcttat ctgtttcctg ccagtacaga
     3781 taatcatccc atgattcctc agaccagatt agtttcactc aataatgtcc ttttctgttc
     3841 ctttgcctga tttcaggcta tcgattgagt ccatcaatct ccgggcgtta gcgggggaac
     3901 gcagtagata agccgtctct tccagcgagt tgtattcttc gagtgacatc agaacacaag
     3961 cctctccatt ctgacgagta ataaggatcg gggcatgatc ttcaacggct ttcatcattg
     4021 ttgccgacaa attctgacgc gcttcgctgt agctaattgt acgcatgtca atctcctctt
     4081 ttgtacagtt cattgtacaa tgatgggcgt taattaacta tttattaatt agtttgtaga
     4141 tcaaggtatt gtcagtgaga cgaaaatcca ggcttcgcta tttttggtgc cgtcagctta
     4201 gaggacagtc ctcttagccc cctcctttcc ccgctcattc attaaacaaa tccatcgcca
     4261 taaaatatat aaaaaagccc ttgctttcta acgtgaaagt ggtttaggtt aaaagacatc
     4321 agttgaataa acattcacag agacttttat gatacgcgtt caatttaaac accaccatca
     4381 tcaccatcat cctgactagt ctttcaggcg atgtgtgctg gaagacattc agatcttcca
     4441 gtggtgcatg aacgcatgag aaagcccccg gaagatcacc ttccgggggc ttttttattg
     4501 cgcggttgat aacggttcag acaggtttaa agaggaataa caaaatgaca gacaacactc
     4561 gtttacgcat agctatgcag aaatccggcc gtttaagtga tgactcacgc gaattgctgg
     4621 cgcgctgtgg cattaaaatt aatcttcaca cccagcgcct gatcgcgatg gcagaaaaca
     4681 tgccgattga tattctgcgc gtgcgtgacg acgacattcc cggtctggta atggatggcg
     4741 tggtagacct tgggattatt ggcgaaaacg tgctggaaga agagctgctt aaccgccgcg
     4801 cccagggtga agatccacgc tactttaccc tgcgtcgtct ggatttcggc ggctgccgtc
     4861 tttcgctggc aacgccagtt gatgaagcct gggacggccc gctctcctta aacggtaaac
     4921 gtatcgccac ctcttatcct cacctgctca agcgttatct cgaccagaaa ggcatctctt
     4981 ttaaatcctg cttactgaac ggttctgttg aagtcgcccc gcgtgccgga ctggcggatg
     5041 cgatttgcga tctggtttcc accggtgcca cgctggaagc taacggcctg cgcgaagtcg
     5101 aagttatcta tcgctcgaaa gcctgcctga ttcaacgcga tggcgaaatg gaagaatcca
     5161 aacagcaact gatcgacaaa ctgctgaccc gtattcaggg tgtgatccag gcgcgcgaat
     5221 caaaatacat catgatgcac gcaccgaccg aacgtctgga tgaagtcatc gccctgctgc
     5281 caggtgccga acgcccaact attctgccgc tggcgggtga ccaacagcgc gtagcgatgc
     5341 acatggtcag cagcgaaacc ctgttctggg aaaccatgga aaaactgaaa gcgctgggtg
     5401 ccagttcaat tctggtgctg ccgattgaga agatgatgga gtgatcgcca tgagctttaa
     5461 cacaatcatt gactggaata gctgtactgc ggagcaacaa cgccagctgt taatgcgccc
     5521 ggcgatctcc gcttctgaaa gcattacccg cactgttaac gatattctcg ataacgtgaa
     5581 agcacgcggc gatgaggccc tgcgggaata cagcgcgaag tttgataaaa ccacggttac
     5641 cgcgctgaag gtgtctgctg aggagatcgc cgccgccagc gaacgcctga gcgatgaact
     5701 aaaacaggcg atggcggtgg cagttaagaa tgttgaaacc ttccacactg cgcaaaaact
     5761 gccgccgata gatgtagaaa cgcagccagg cgtgcgttgc cagcaggtca cgcgcccggt
     5821 cgcttcagtt gggttgtata ttcctggcgg ctccgccccg ctcttctcaa cggtattaat
     5881 gctggcgact ccggcgcgta ttgcgggctg taaaaaagtg gtgctgtgct caccgccgcc
     5941 gattgccgat gagatccttt atgcggcgca gctgtgcggt gtgcaggacg tgtttaacat
     6001 cggcggcgca caggccattg ccgcgctggc gtttggtacg gaatctgtgc cgaaagtgga
     6061 caaaatcttc gggccgggta acgcctttgt caccgaagca aaacgccagg taagccagcg
     6121 tctggacggt gcggcgatcg atatgcccgc aggcccgtcg gaagtgctgg tgattgctga
     6181 cagcggcgct acgccggatt tcgtggcttc tgatttgctt tctcaggctg aacacggccc
     6241 ggactcacag gtgattttac tgacgcccga tactgatatg gcgcatcaag ttgccgaggc
     6301 cgtcgaacgc caactggcag aactgccgcg tgccgaaacc gcccgccagg cactgagcgc
     6361 cagccgcctg atcgtgacta aagatttagc gcagtgcgta gagatctcca accagtacgg
     6421 cccggagcac ctgatcattc agacccgcaa cgcccgcgaa ctggtcgatg gcatcaccag
     6481 cgccggttcg gtatttcttg gtgactggtc accggaatcg gcaggcgact atgcctccgg
     6541 cactaaccat gttctaccga cttacggtta caccgccacc tgttccagcc tcgggctggc
     6601 agatttccag aagcgcatga ccgtacagga actgtcgaaa gaggggttct ccgcgctggc
     6661 ttcaaccata gaaacactgg ccgccgccga gcgcctgacc gcccacaaaa atgccgttac
     6721 tttgcgtgtt aacgccctta aggagcaagc atgagcaccg tgactattac cgatttagcg
     6781 cgtgaaaacg tccgcaacct gacgccgtat cagtcggcgc gtcgtctggg cggtaatggc
     6841 gacgtctggc tgaacgccaa cgaatatccc acagccgtgg agtttcagct tactcagcaa
     6901 acgctcaacc gctacccgga atgtcagccg aaagcggtga ttgaaaatta cgcgcaatat
     6961 gcaggcgtaa aaccggaaca ggtgctggtc agccgtggcg cggacgaagg tattgaactg
     7021 ctgatccgcg ctttttgcga accgggtaaa gactccatcc tctactgccc accaacgtac
     7081 ggcatgtaca gcgtcagcgc cgaaacgatt ggcgtcgagt gccgcacagt gccgacgctg
     7141 gacaactggc aactggactt acagggcatt tccgacaagc tggacggcgt aaaagtggtc
     7201 tatgtttgca gccccaacaa cccgaccggg caactgatca atccgcagga ttttcgcacc
     7261 ctgctggagt taacccgcgg taaggcgatt gtggttgccg atgaagccta tatagagttt
     7321 tgtccacagg catcgctggc tggctggctg gcggaatatc cgcacctggc tattttgcgc
     7381 acactgtcga aagcttttgc tctggcgggc cttcgttgcg ggtttacgct ggcaaacgaa
     7441 gaagtcatca acctgctgat gaaagtgatc gccccctacc cgctctcgac gccggttgcc
     7501 gacattgcgg cccaggcgtt aagcccgcag ggaatcgtcg ccatgcgcga acgggtagcg
     7561 caaattattg cagaacgcga atacctgatt gccgcactga aagagatccc ctgcgtggag
     7621 caggtttttg actctgaaac caactacatt ctggcgcgct ttaaagcctc cagtgcagtg
     7681 tttaaatctt tgtgggatca gggcattatc ttacgtgatc agaataaaca accctcttta
     7741 agcggctgcc tgcgaattac cgtcggaacc cgtgaagaaa gccagcgcgt cattgacgcc
     7801 ttacgtgcgg agcaagtttg atgagtcaga agtatctttt tatcgatcgc gatggaaccc
     7861 tgattagcga accgccgagt gattttcagg tggaccgttt tgacaaactc gcctttgaac
     7921 cgggcgtgat cccggaactg ctgaagctgc aaaaagcggg ctacaagctg gtgatgatca
     7981 ctaatcagga tggtctggga acacaaagtt tcccgcaggc ggatttcgat ggcccgcaca
     8041 acctgatgat gcagatcttc acctcgcaag gcgtacagtt tgatgaagtg ctgatttgtc
     8101 cgcacctgcc cgccgatgag tgcgactgcc gtaagccgaa agtaaaactg gtggaacgtt
     8161 atctggctga gcaagcgatg gatcgcgcta acagttatgt gattggcgat cgcgcgaccg
     8221 acattcaact ggcggaaaac atgggcatta atggtttacg ctacgaccgc gaaatcctga
     8281 actggccaat gattggcgag cagctcacta aacgagaccg ttacgctcac gtagtgcgca
     8341 acaccaaaga gacgcagatt gacgttcagg tgtggctgga tcgcgaaggt ggcagcaaga
     8401 ttaacaccgg tgttggcttc tttgatcaca tgctggatca gatcgccacc cacggcggtt
     8461 tccgtatgga aatcaacgtc aaaggcgacc tctatatcga cgatcaccac accgtcgaag
     8521 ataccggcct ggcgctgggt gaagcgttaa aaattgccct tggcgacaaa cgcggtattt
     8581 gccgctttgg ttttgtgcta ccgatggacg aatgccttgc ccgctgcgcg ctggatatct
     8641 ctggtcgccc gcacctggaa tataaagccg agtttaccta ccagcgcgtg ggcgatctca
     8701 gcaccgaaat gatcgagcac ttcttccgtt cgctctcata caccatgggc gtgaccctgc
     8761 acctgaaaac caaaggtaaa aacgatcacc accgtgtaga gagcctgttc aaagcttttg
     8821 gtcgcaccct gcgccaggcc atccgcgtgg aaggcgatac cctgccctcg tcgaaaggag
     8881 tgctgtaatg aacgtggtga tccttgatac tggctgcgcc aacctgaact cggtgaagtc
     8941 tgccattgcg cgccacggtt atgaacccaa agtcagccgt gacccggacg tagtgttgct
     9001 ggccgataaa ctatttttac ccggcgtagg caccgcacaa gcagcgatgg atcaggtgcg
     9061 tgagcgcgaa ctgtttgatc tcatcaaagc ctgtacccaa ccggtgctgg gcatctgctt
     9121 agggatgcaa ctgcttgggc ggcgcagcga agagagcaac ggcgtcgatt tgctgggcat
     9181 catcgacgaa gacgtgccga aaatgaccga ctttggtcta ccgctgcccc atatgggctg
     9241 gaaccgcgtt tacccgcagg caggcaaccg cctgtttcag ggaattgaag acggcgcgta
     9301 cttttacttt gttcacagct acgcaatgcc ggttaatccg tggaccatcg cccagtgtaa
     9361 ttacggcgaa ccgttcaccg cggcggtaca aaaagataac ttctacggcg tgcagttcca
     9421 cccggagcgt tctggtgccg ctggcgctaa gctgctgaaa aacttcctgg agatgtgatg
     9481 attattccgg cattagattt aatcgacggc actgtggtgc gtctccatca gggtgattat
     9541 ggcaaacagc gcgattacgg taacgacccg ctgccgcgct tacaggatta cgccgcgcag
     9601 ggtgccgaag tgctgcacct ggtggatctg accggggcaa aagatccggc taaacgtcaa
     9661 atcccgctga ttaaaactct ggtggcgggc gttaacgttc cggtgcaggt tggcggcggc
     9721 gtgcgtagcg aagaagacgt ggcggcgtta ctggaagcgg gcgttgcgcg cgtggtggtc
     9781 ggctccaccg cggtgaaatc accagaaatg gtgaaaggct ggtttgaacg tttcggtgcc
     9841 gatgccttag tgctggcgct ggatgtccgt attgacgagc aaggcaacaa gcaggtggcg
     9901 gtcagcggct ggcaagagaa ttcgggtgtg tcgctggaac aactggtgga aacctatttg
     9961 cctgtcggcc tgaaacatgt gctgtgtacc gatatctcgc gcgacggcac gctggcaggt
    10021 tctaatgtct ctttatatga agaagtgtgc gccagatatc cgcaggtggc atttcagtca
    10081 tccggcggca ttggcgacat taacgatgtt gctgccctgc gtggtactgg cgtgcgcggc
    10141 gtaatagttg gtcgggcatt actggaaggt aaattcaccg tgaaggaggc catcgcatgc
    10201 tggcaaaacg tataattccg tgtctcgacg ttcgtgatgg tcaggtggtg aaaggcgtac
    10261 agtttcgcaa ccatgaaatc attggcgata tcgtgccgct ggcaaaacgc tacgccgaag
    10321 aaggtgcaga cgaactggtg ttctacgata tcaccgcttc cagcgatggc cgtgtggtag
    10381 ataaaagctg ggtatctcgc gtggcggaag tgatcgacat tccgttctgt gtggcgggtg
    10441 ggattaagtc tctggaagat gccgcgaaaa ttctttcctt tggcgcggat aaaatttcca
    10501 tcaactcgcc tgcgctggcg gacccgacgt taattactcg cctggccgat cgctttggcg
    10561 tgcagtgtat tgtggtgggt attgatacct ggtacgacgc tgaaaccggt aaatatcatg
    10621 tgaatcaata tactggcgat gaaagccgca cccgcgtcac tcagtgggaa acgctcgact
    10681 gggtacagga agtgcaaaaa cgcggtgccg gagaaatcgt cctcaacatg atgaatcagg
    10741 acggcgtgcg taacggttac gacctcgaac aactgaaaaa agtgcgtgaa gtttgccacg
    10801 tcccgctaat tgcctccggt ggcgcgggca ccatggaaca cttcctcgaa gccttccgcg
    10861 atgccgacgt tgacggcgcg ctggcggctt ccgtattcca caaacaaata atcaatattg
    10921 gtgaattaaa agcgtacctg gcaacacagg gcgtggagat caggatatgt taacagaaca
    10981 acaacgtcgc gaactggact gggaaaaaac cgacggactt atgccggtga ttgtgcagca
    11041 cgcggtatcc ggtgaagtgt taatgctggg ctatatgaac ccggaagcct tagataaaac
    11101 catcgaaagc ggcaaagtca ccttcttctc gcgcactaaa cagcgactgt ggaccaaagg
    11161 cgaaacgtcg ggcaatttcc tcaacgtagt aaatattgcg ccggactgcg acaacgacac
    11221 gctactggta ctagcgaatc ctatcggacc aacctgccac aaaggcacca gcagctgctt
    11281 cggcgacacc gctcaccagt ggctgttcct gtatcaactg gaacaactgc tcgccgaacg
    11341 caaatctgcc gacccggaaa cctcttacac tgccaaactg tatgccagcg gcaccaaacg
    11401 cattgcgcag aaagtaggtg aagaaggcgt ggaaaccgca ctggcagcga cggtacatga
    11461 ccgctttgag ctgaccaacg aggcgtctga tttgatgtat cacctgctgg tgctgttgca
    11521 ggatcaggat ctggacttaa cgacggtgat tgagaacctg cgtaaacggc atcagtaagt
    11581 tgcgggtgat cggatgcgat gttgttgtcg catccggcaa aaaaaacggg caaggtgtca
    11641 ccaccctgcc ctttttcttt aaaaccgaaa agattacttc gcgttgtaat tacgcagagc
    11701 gttacgcccc agcacaatcc ccgcaccaac catgccgccc agcagtaccg ccagaatcaa
    11761 agtgattgcc tttttcgggc tatcgcgacg aataggtaac gtcggtttca tcacatagag
    11821 gtaagcatga atatcaagat catcaacttt taagctttcg atatccagca ggttttgacg
    11881 tgtctgatag tagtttggtg agaacaccaa cggacgagtc gcttcatgct taatcatcga
    11941 ctccagcgct tcgctcccca aaaggaacaa cgtatcttgt gtcacatctt cagtctgctg
    12001 aacctgtggc tttgtcacct gcgcctgatt cgcatactgc aacgcttcct gaatctgacg
    12061 gatacgcaga tctttctgct cctgcgcaac tacttcctga gttttcagag aatcctgcaa
    12121 gtttttcata cgctgcgcga tgttgtcttt caggtctttt cccagttctt catttacctg
    12181 gtcgtcaact tgctggatat acttcgccag ctgcttctga gcaccttcag gtgtttcacc
    12241 aacataagac accgctaaag gtaatgactg atttttgacg gtaggctcaa tggtcagctt
    12301 ttctgcttct tcctggttat ccagcgtttc cgctaatgct gagaatgcag tgctgtaacg
    12361 tccgataagt gaagcctgaa tgtcggagac tttcggtaca gccgatccgt agatcacatt
    12421 catcgcgttg gtataaccag cgatttgccc cacatcaggt tgagtaacga tagctgtgga
    12481 agtccacttt tctttggcaa cggccagata ccctatagcc agggcaatag caacaatgac
    12541 agagatgata attgtcatct tgccacgcca caactgcacc agtaaatcaa tcaaatcaat
    12601 ctgttccggg tcatggtttt gcccagaaac attattattt tctactctca tcattaccct
    12661 aacggacgaa ataattttaa gtcgccatag tttagcgata atcgccatga atgctatagg
    12721 ataaatgata aaaataatga attattacaa agggagcata agacaatcag ggcgtaaata
    12781 gccctgataa caagatgtta gtcgctgcca aagagatcgc gggtgtatac cttatctgcc
    12841 acatctttaa gctcttctgc cattcggtta gagataatga cgtcggcttg ttgtttgaag
    12901 atggcgagat cacgttccag gcgagagttg aagaatgagt cttctttcat cactggctcg
    12961 tagatgatca cttcaacgcc tttcgccttg atacgtttca taatcccctg aatagaagac
    13021 gcacggaagt tatctgaacc gctcttcata atcagacgat aaatacccac cacttgcggt
    13081 ttgcgtgaca aaatggcatc ggcaataaaa tccttacgcg tgcggtttgc atcgacaatt
    13141 gccgagatca ggttattcgg cacagactga tagttcgcca gtaactgctt ggtatctttc
    13201 ggcaaacaat aaccaccata accaaacgac ggattgttgt aatggttgcc aatacgcgga
    13261 tcgagacaaa cgccttcgat aatctggcga gtattcagac ctaaactttc tgcatagcta
    13321 tccagttcat taaagtatgc tacgcgcatc gccagatagg tattagcaaa cagttttatc
    13381 gcttctgctt cagtggagtc ggtaaacagg gtcgggatat tttgcttaat cgcgccttcc
    13441 tgtaacaacg cagcaaaacg ttctgcgcgt tctgaacgct caccaatgac aatacgtgaa
    13501 ggatggagat tatcgtaaag ggctttacct tcgcggagga attcagggga gaatataata
    13561 ttttcagtgc gatatttctt atgcatcgct gcagtaaaac caacgggaac cgttgatttg
    13621 atcaccataa ccgcataagg atttatctca actacgtctt taattactga ttctacactg
    13681 gatgtattga aataattagt tttaggatca tagtcggttg gagtggcgat gatgacataa
    13741 tcagcatacc ggtaggcttc atttttatct aatgtagcat taaagtgtat tttatctgat
    13801 tgcaaaaact gctgaatttc cttatcaaca ataggagata tccgatcatt cagcatagca
    13861 acgcgtgacg gtaaaatatc taatgccaca acctcatgat tttgtgcgat tagaagcccg
    13921 tttgacaagc ctacatagcc ggtaccggaa atggtgattt tcatttcatg ctctcagaat
    13981 taacttaact gtgaatcatg atgtttttag catcctgata agagctaaaa gttttaacgc
    14041 tacgttgtca gatggtcaac gcaacgaaaa acaattcaga ttttctttat aagaatagct
    14101 gatatttatt aagttaatat taagcagtga aatttagtct ataagataat tggcaaaaaa
    14161 aagcccggcg caatatacgc caggcctcaa tttattgtta gttaaataag attaatccag
    14221 ccattcagta tggaacacac cttctttatc aatgcgctta taagtatgtg caccgaaata
    14281 gtcacgctgt gcctgtatca ggttcgcagg cagaacagcg gcacggtagc tgtcataata
    14341 ggcaaccgca gcggcgaagg tgggaaccgg gataccgttc tgtactgcat aagcaacaac
    14401 atcacgcagc gcctgctggt agtcatcggc aatttgcttg aagtacgggg ccagcagcag
    14461 gttagcgatc tgcggatttt cggcatatgc atcggtgatt ttctgcagga actgcgcacg
    14521 gatgatgcag ccagcacgga aaatcttcgc gatttcgccg tagttcagat cccagttgta
    14581 ctcttcagac gcagcacgca gctgagagaa gccctgagcg taagaaacga ttttgcccag
    14641 atacagcgca cgacgaactt tttcgatgaa ctcagccttg tcgccagctg gctgcgcttg
    14701 cggaccagag agaactttag atgcggcaac gcgctgatct ttcagagaag agatataacg
    14761 tgcaaacaca gactcggtaa tcagcgacag cggttctccg agatccagcg cgctctggct
    14821 ggtccattta ccggtacctt tgttagccgc ttcatccagg atcacatcaa ccaggtagtt
    14881 accgtcttca tcttttttag tgaagatgtc tttggtgatg tcgatcaggt agctgctcag
    14941 ttcaccgtta ttccactcgg taaaggtctg tgccagttct tcgttagaga gattcaggcc
    15001 gcctttaagc agagaatagg cttcagcaat cagctgcata tcaccgtatt caataccgtt
    15061 atgaaccatc ttcacatagt gacctgcacc atcggcacca atataggtaa cgcatggctc
    15121 cccatcttca gctactgcgg cgattttggt caggattggt gcaaccagtt cataggcttc
    15181 tttctgccca ccaggcatga tggaaggtcc tttcagcgca ccttcttcac caccggaaac
    15241 accggtaccg ataaagttaa aaccttcggc agaaagctca cggttacgac gaatggtgtc
    15301 ctggaagaag gtgttaccac catcaatgat gatgtcacct ttatcgaggt atggcttgag
    15361 ggaatcaata gcagcatccg tgcctgcgcc tgctttcacc attaacagga tgcgacgagg
    15421 cgtttccaaa gattcaacaa actctttcac cgtatagtaa ggaaccagtt tcttgcctgg
    15481 attttcggca ataacttctt ccgtcttctc acgggagcgg ttgaaaatag agacggtata
    15541 accacggctt tcgatgttga gcgctaggtt gcgccccatc actgccatac cgactacgcc
    15601 gatctgttgc tttgacattg tttactcctg tcaggggtta ctgcgaactt taacgcaggt
    15661 ctatatgaag cttacggtaa ctcaggttag aaaagtatta tatttttact gacgctaagc
    15721 aattcagttt ttctcagaaa atttataacc atcaggattg ctttgttgcc aattccaggc
    15781 atcgcgaacc atctcatcta atcccaaaca ggctttccat ccaagttcaa ggtatgcttt
    15841 agacgggtcg gaccaacatt cagcaatgtc gcctgctcgg cgctcaacca acacataagg
    15901 tatattaaca gaggttatcc gttgaaaagc agccaaaagt tcaagaaccg aataaccatg
    15961 ccctgtgcct aaattgtaaa ccttataatt gctaccttca tctcggtgct tcaaggcagc
    16021 tatatgaccg tttgcgagat ccataacatg aataaaatct ctaacgccag taccatcttt
    16081 agtttcataa tcattaccat agatggataa ttgcttatat ttaccacttg caactgaaca
    16141 tatataaggc atcaaattat ttggaatacc gttagggtct tctccaattt ttccggaagg
    16201 atgtgcgcca acaggattaa aataacgcaa aattgttgtt ctaaaaaaag gatatgccaa
    16261 agaaacatca tgtaatatgt tctcaaccat aagttttgaa gtcccatagg gattagtagt
    16321 gccacctaca gggaatgttt cttttacggg tatcttctct ggagtaccat atacagtggc
    16381 tgaggagctg aatattagat tcttgatatt attactaatc atttcctcaa taagaacaag
    16441 tgtaccgtat acattattat cgtagtactc tgttgggaat tgaattgaat cgcttactga
    16501 tttcaggcca gcaaaatgaa taacatctgt tatgctgttt tcagtaaaga tggattttaa
    16561 aataacacga tctcgaatat caccattaat gatacagata tcactaccag taatttcttt
    16621 aacgcgatgc aaagattcaa ctgaagaatt atgaaaatta tcaagcacta ttacttcata
    16681 ttgttctttc agcagcatta atacagtatg tgagccaata tatccagcac cacccgtcac
    16741 taatatagcc atacaacagc accctcattt aatcaaagca acaagactag cagatagaat
    16801 gttattaaag cttggataat ttaataactc ttatgcagtt ataatattaa ctaaagaatt
    16861 tgcagattca ctttcattaa attttgacaa tgcgaatctt cgactattta atcccatagt
    16921 gacaataagc tcacgttcat gaataaatgt ttccataact aaagcaagtt tttgtgtatc
    16981 ccatggaggt acaagatatc cattatatcc atcaattact gtttctcgac aacctggcac
    17041 atcggtcgta ataatcgcac gacctattgc cattgcttcc tgagtacttt tggggacacc
    17101 ttctcgataa gaaggtaata caaagacgct gatatcactc aaatattctt ttatataaga
    17161 gcacggtcct aaaaattcaa cataaccttc cgctttccaa tttacaattt gtgcagcatc
    17221 aattccaccc ggattatcat caaaatcacc agctatacta aatcggacca ttggatattt
    17281 tttctttatt attttagccg cttcaacaaa ttcacatacg cccttctctc tcaataatct
    17341 tgccaccata ccaaaatgga tctccccaca aattggtgga tgataactaa attcattgag
    17401 atttacacct atgccaccaa taactttata atctttga
//
LOCUS       DAZFXT010000097        17181 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.097, whole genome shotgun sequence.
ACCESSION   DAZFXT010000097 DAZFXT010000000
VERSION     DAZFXT010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.097"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..111
                     /locus_tag="ACHKW0_003488"
     CDS             <1..111
                     /locus_tag="ACHKW0_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7476265.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            173..442
                     /locus_tag="ACHKW0_003489"
     CDS             173..442
                     /locus_tag="ACHKW0_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7476266.1"
                     /translation="MSEQYLITLDEWKPKRFSLPITNTTLVKYGKLGYIVPRPQKIRG
                     RWLIDRRAVFVGPGETGIAPEIHTGDDDALKEILTHVTEATKKQH"
     gene            411..1529
                     /locus_tag="ACHKW0_003490"
     CDS             411..1529
                     /locus_tag="ACHKW0_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO7476267.1"
                     /translation="MSPRPRKNSTDVAGLYEKFDRRTGRVYYQYKNPVTGKFHGLGTD
                     KGKAEKIASTANQRIAAAEAEYFMRKIDESPSATKRRGIRLKAWVDRYLKIQDTRLKN
                     GDIAATTHKEKARMAAYLVSRLGNHPLKELEVRDFALILDEWLDKDMVSTARVNRGLW
                     VDIYKEAQHAGEVPPGWNPPEATRKPIPKVTRARLTLEDWQKIYNATPEKHFIRNAML
                     LAIVTGQRRDDICHMRFSDVWNEHLHITQGKTGMRLALPLTLRCDAIGITLKEVIDGC
                     RDRILSPYLIHSRHQKQPKPMSKDNLSDYFAKARDLAGITPPAGKTPPTFHEQRSLSE
                     RLYRAQGIDTKTLLGHKVQATTDRYNDTRGQEWVKLVV"
     gene            complement(1606..2073)
                     /locus_tag="ACHKW0_003491"
     CDS             complement(1606..2073)
                     /locus_tag="ACHKW0_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2335 domain-containing protein"
                     /protein_id="HFO7476268.1"
                     /translation="MPDQKESENTKLTCEEQKDNELVSRVIENPEVLNRVLDSPQVRA
                     IVCQHFQGPVPPPSMLKKYDQLVPGLANRLVELTEKEQAHRHKTVADSIDIARDGQTK
                     AFWLAILIILAATVFGVMGETVLAGTLVSIDLVALVTAFIVGKHYSKQEPDQD"
     gene            complement(2051..2254)
                     /locus_tag="ACHKW0_003492"
     CDS             complement(2051..2254)
                     /locus_tag="ACHKW0_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009433571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476269.1"
                     /translation="MKRKSVALLQAIGSVIALSPTVVSANGMPANPVSALRQDAAAIR
                     GDFQKAMVSVNVEKLNNARSKRK"
     gene            2563..3357
                     /gene="potC"
                     /locus_tag="ACHKW0_003493"
     CDS             2563..3357
                     /gene="potC"
                     /locus_tag="ACHKW0_003493"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HFO7476270.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            3354..4400
                     /gene="potD"
                     /locus_tag="ACHKW0_003494"
     CDS             3354..4400
                     /gene="potD"
                     /locus_tag="ACHKW0_003494"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HFO7476271.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLSNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            4458..4919
                     /locus_tag="ACHKW0_003495"
     CDS             4458..4919
                     /locus_tag="ACHKW0_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HFO7476272.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            4916..5704
                     /gene="ycfZ"
                     /locus_tag="ACHKW0_003496"
     CDS             4916..5704
                     /gene="ycfZ"
                     /locus_tag="ACHKW0_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HFO7476273.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWYQGINNALAVLQAQILKQPT
                     PPLTYYPHPGQQHENAPPGSTNTLGFIAWAATFILFSWIFCYTTRFIYALKFAVAMTI
                     ANMGYQALCLYIDNSFAITRISPLWAGLIGVCTFIAALLLTSKR"
     gene            complement(5843..6664)
                     /gene="cobB"
                     /locus_tag="ACHKW0_003497"
     CDS             complement(5843..6664)
                     /gene="cobB"
                     /locus_tag="ACHKW0_003497"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD+-dependent deacetylase"
                     /protein_id="HFO7476274.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(6680..7591)
                     /gene="nagK"
                     /locus_tag="ACHKW0_003498"
     CDS             complement(6680..7591)
                     /gene="nagK"
                     /locus_tag="ACHKW0_003498"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045127 - N-acetylglucosamine kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HFO7476275.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(7620..8864)
                     /gene="lolE"
                     /locus_tag="ACHKW0_003499"
     CDS             complement(7620..8864)
                     /gene="lolE"
                     /locus_tag="ACHKW0_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HFO7476276.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(8864..9565)
                     /gene="lolD"
                     /locus_tag="ACHKW0_003500"
     CDS             complement(8864..9565)
                     /gene="lolD"
                     /locus_tag="ACHKW0_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HFO7476277.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(9558..10757)
                     /gene="lolC"
                     /locus_tag="ACHKW0_003501"
     CDS             complement(9558..10757)
                     /gene="lolC"
                     /locus_tag="ACHKW0_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HFO7476278.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRMMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGNEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            11019..12092
                     /gene="ycfT"
                     /locus_tag="ACHKW0_003502"
     CDS             11019..12092
                     /gene="ycfT"
                     /locus_tag="ACHKW0_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HFO7476279.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFTPPSLPAAVSYSR"
     gene            12220..15666
                     /gene="mfd"
                     /locus_tag="ACHKW0_003503"
     CDS             12220..15666
                     /gene="mfd"
                     /locus_tag="ACHKW0_003503"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HFO7476280.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEATNLLPAHEFP
                     TDKAAIELFRSQWRDIFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQTDTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            15810..16772
                     /gene="ldtC"
                     /locus_tag="ACHKW0_003504"
     CDS             15810..16772
                     /gene="ldtC"
                     /locus_tag="ACHKW0_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HFO7476281.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(16855..17112)
                     /gene="bhsA"
                     /locus_tag="ACHKW0_003505"
                     /gene_synonym="ycfR"
     CDS             complement(16855..17112)
                     /gene="bhsA"
                     /locus_tag="ACHKW0_003505"
                     /gene_synonym="ycfR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HFO7476282.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         4368 a         4404 c         4271 g         4138 t
ORIGIN      
        1 gctattccat tcgaaggtga gcgccataat gcacttgatg acgcccgtta ccaggcaaaa
       61 tacgtttcag ttatctggca aaaactgatc ccgagtcagg ctgattttta atgttcaacc
      121 gtcgccagtt gtcgttgata ttctgcaact ggcgcgttcc ggagtgatag ccatgagcga
      181 acagtacctg ataacgctcg acgagtggaa accaaaacgg ttcagtctcc caataacaaa
      241 cactaccctg gtgaaatacg gaaaactagg atacatcgtt ccaagaccac aaaaaattcg
      301 tgggcgttgg ctgatagatc gccgagcagt atttgttggg cctggtgaaa cgggaattgc
      361 gccggaaatt catactggcg atgatgatgc actgaaggag attttaactc atgtcaccga
      421 ggccacgaaa aaacagcact gacgtagccg gtctttacga aaagtttgat cgcagaactg
      481 gcagagttta ctaccagtat aaaaatcctg tgactggaaa atttcacgga ctcggaacag
      541 acaaaggtaa ggcagaaaaa atcgcttcca cagccaatca gcggatagct gcagcagaag
      601 ctgaatattt catgcgcaaa attgatgaaa gtccgtcagc aacaaaacgt cggggtatca
      661 gattaaaggc atgggttgat cgatatctga aaatacagga cacgcgactg aaaaatggag
      721 atattgcagc tacaactcac aaagaaaaag cccgaatggc tgcatacctg gtttcccgtc
      781 tgggaaacca cccattgaaa gaactggaag taagagactt tgcattaata ctggatgagt
      841 ggctggataa agacatggtc agcacagcga gagtaaatcg tggattatgg gttgatattt
      901 ataaagaagc acagcatgca ggggaagttc ctcctggatg gaatcctccg gaggctaccc
      961 gtaaaccgat ccctaaagta actagagcca ggctcaccct ggaagactgg caaaaaattt
     1021 acaacgcaac gcctgaaaaa cactttatcc gtaacgcaat gcttcttgcg attgttactg
     1081 gtcagcgccg tgatgacatt tgccacatgc gtttttcaga tgtgtggaac gaacacttgc
     1141 atatcaccca gggaaaaacc ggaatgcgtc tggcgttacc gcttacacta cgctgtgatg
     1201 ccattgggat aacgttaaaa gaagttattg atgggtgccg agacagaata ttaagtccat
     1261 atctaatcca tagtcggcac cagaaacaac caaagccgat gagtaaagac aacctgagcg
     1321 actactttgc caaagcacgg gatctggctg ggataactcc accagcagga aaaactccgc
     1381 caacatttca tgaacaacgc tccctgtcag aacgactgta ccgcgcgcag ggtattgata
     1441 caaaaacatt actgggacat aaagtccagg caaccaccga tcgttataac gatactcgtg
     1501 gtcaggaatg ggttaaattg gttgtttgac gaaataaatg cagctgaaaa atgttgatat
     1561 tgctttgcta gtgtaaaaac cccggcatca accggggttc ggagactaat cttgatcagg
     1621 ttcctgctta gaataatgtt ttccaacaat aaatgccgta accaatgcaa caagatctat
     1681 tgaaacgaga gttccggcaa gaactgtctc ccccatgacg ccaaagacag tggcagctaa
     1741 gatgatcaat attgccaacc aaaaagcctt tgtttgacca tctctggcaa tatcaatgct
     1801 atcagccact gttttatggc gatgagcctg ctctttttcg gtcaactcaa caagtcgatt
     1861 tgcaagccca ggcaccagtt gatcatactt tttaagcatt gaaggtggcg gaacaggccc
     1921 ctgaaaatgc tggcaaacaa tagcccgcac ctgcggacta tccaaaaccc tgtttaagac
     1981 ctctggattt tcgattactc gagaaaccag ttcattatcc ttttgttctt cacaagtgag
     2041 tttggtgttc tcactttctt tttgatctgg cattgttcag cttttcaacg ttaacggata
     2101 ccattgcttt ttggaaatcg cctctgatag ccgcagcatc ctgacgcaaa gcggaaactg
     2161 gattcgctgg catgccattt gctgaaacga ccgtcgggct cagggctata accgagccaa
     2221 tggcttgtaa caaagccaca cttttacgct tcatagtcac tcctgagttc aatgggattc
     2281 aaaacggatt caaagtgaat cttgatagct aattctaata ccacattatg tggtttttca
     2341 acacgaagaa atcacatcaa aaatctcatt cggaaagcga tatgggtaaa caaactggaa
     2401 tacttttctg tcctagtaag ctcacccatg tataaaaatg ctgtaaaatg cgtggtcctg
     2461 ttttggggaa aagttttgga gagattttgg agaaaggaaa aaattgaata aattcaaaat
     2521 cctgaaagtg tcccgcctta tgaataaaaa ggtggaactc gaatgatcgg tcgactgctt
     2581 cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat tattttgatt
     2641 gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac caccaaatgg
     2701 tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc actaacaatg
     2761 gcggtgtttt cggcgacgtt tgctacgctt atcggttcac tgacggcagt tgcgctgtac
     2821 cgttatcgct ttcgcggtaa gccgttcgtt agcggaatgc tgtttgtggt gatgatgtcg
     2881 ccagatattg tgatggcgat ttctctgctg gtgctgttta tgctgctggg tattcagctt
     2941 ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt ggtggtgacg
     3001 gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa agatctcggt
     3061 gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc tgcggtggcg
     3121 gcgggctggg tgttaagctt taccctgtcg atggacgatg tagtagtttc ttcgttcgtc
     3181 accggaccaa gctatgaaat tctgccgtta aaaatttatt cgatggtcaa agtcggcgta
     3241 tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt gatggtaatt
     3301 gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt taaatgaaaa
     3361 aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc gccgctcacg
     3421 ccgatgacaa caatacgctg tatttctaca actggaccga gtacgtgccg ccaggactgc
     3481 ttgaacagtt caccaaagaa accggtatta aggttatcta ttcgacttac gagtcgaacg
     3541 aaaccatgta cgccaagttg aaaacgtaca aagacggtgc ctacgacctg gtggttcctt
     3601 caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc gacaagtcga
     3661 agttaagcaa tttcagcaac ctcgatccag acatgctcaa caagccgttt gacccgaata
     3721 acgactactc cattccgtat atctgggggg cgacggcgat tggcgttaac ggtgatgcgg
     3781 tagatccgaa atccgttacc agctgggccg atctgtggaa gcctgagtac aaaggtagcc
     3841 tgctgctgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgcaaa ctgggctact
     3901 ccggtaacac taccgatccg aaagagattg aagctgcata taacgagctg aaaaaactga
     3961 tgccaaacgt cgcagcgttt aactccgata acccggctaa cccgtacatg gaaggcgaag
     4021 ttaacctcgg catgatctgg aacggctctg ctttcgttgc acgccaggcg ggtacgccaa
     4081 ttgacgtggt gtggccgaaa gaaggcggca ttttctggat ggacagcctg gcgatcccgg
     4141 caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg cgcccggatg
     4201 tggcaaaaca ggttgccgaa actatcggtt atccaacgcc aaaccttgcg gcgcgtaagc
     4261 tgttaagccc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa accattaaaa
     4321 atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag tattatcaga
     4381 agctgaaagc aggacgttaa ttcagcaaac gggcggtggt attaccgccc gttttttagt
     4441 tagcaaggat gtggctaatg agtcaggaca gtaaagtttt atcccgcgct tttctcggta
     4501 tcggtctgat attgctcgtt atttctgcaa tcatctttta ttaccatttt acttttacca
     4561 aaagcgcggt tcatacagaa ggaatcattg ttgatgccgt ttggtacaac aatcactcaa
     4621 acgacgtcga tgataatggt tcctggtatc cagtggttgc ctttcgccct acgccagatt
     4681 acacgctaat tttcaattcg aatatcggta gtgattttta tgaagatagc gaaggtgaca
     4741 gagtcaatgt ctattatccg ccagggcatc cagaacaagc cgaaataaac aatccctggg
     4801 ttaatttttt taaatggggc tttgttggca tagcaggcat tattttcggt agtgtcggtc
     4861 tgataatcac gctcccctcc accaaaaaat ctcgcaggaa acggaagtcc cgcccatgaa
     4921 aaaattcatt atactgctca gtttactgat actcctgcct ctggtagcaa ccagtaaacc
     4981 attgattcca ataatgaaga cactttttac ggatgttacc ggtactgtac cggatgctga
     5041 agagatcgca cgtaaagccg agctttttcg ccaacaaacg ggaattgcac cgtttattgt
     5101 gattttaccc gacattaata atgaagccag tctcagacaa aatggtaaag cgatgctggc
     5161 gcatgcgtca tcttcattga gtaatgtaaa aggaagtgtt ctgttactat ttactacccg
     5221 cgaaccacgg ttaattatga tcaccaacgg ccaggttgaa agtggtctgg acgataaaca
     5281 tctcggcctt ctgatagaaa atcacacgct ggcttattta aatgcagatc tctggtatca
     5341 gggaatcaat aatgcattgg ctgttctaca agcacagata ttaaaacaac cgacgccgcc
     5401 actgacgtat tatccgcatc caggacaaca gcatgaaaat gctccccccg gcagcaccaa
     5461 cacattaggt tttatcgcct gggctgcgac gtttatctta ttttcctgga tcttttgcta
     5521 cacgactcgt tttatttatg cgttaaaatt tgcagtggcg atgactattg ccaatatggg
     5581 ttatcaggct ttatgtttgt atatcgataa tagctttgcg attaccagaa tatcgccact
     5641 ctgggcaggg ctaattggtg tctgtacgtt tattgccgca ttgttattga cgagtaaacg
     5701 ataaaagagg ccggaaaagt gcgcgtgtat tatgccggat ggggcgcgaa cgccttatcc
     5761 ggcccacaaa acccgcaaat tcaattaatt gcgtcccctt gtaggcctga taagcgtagc
     5821 gcatcaggca atgcttcctg ctttataatc ccttcagcaa cttttcaaca aactcaggca
     5881 ccacctggct tgccgggccg taatatttct cggcaaattc attaccgacc tgacttggtt
     5941 cgagattcag ttccacggtg tgcgcgccat gcagtttcgc ttcgtgaaca aacccagccg
     6001 ccggataaac atgcccggaa gtaccaatgg caatgaaaat atcagccatc gacaacgcca
     6061 tataaatttc atccatgccg agtggcattt cgccaaacca aacgacgtgc gggcgcaagg
     6121 gtgccgggaa ctggcaacaa tggcatttat cttctggcgt aacgtctccg gtccagtcga
     6181 gaacctgacc actttgcgaa caacgaactt tcagcaattc cccatgcata tgaatgacat
     6241 tggtattacc tgcgcgttca tgcaggttgt ctatattctg cgttaccagc aaaaagcgat
     6301 cgccgagggc gtcttgcagt ttagccagcg caagatgcgc ggcgttaggc tgaatttctg
     6361 gctgctgcag ctgtcgacga cgggcgttat aaaacgcttg caccagttca ggatcgcgat
     6421 cgaaaccttc cggcgttgcc acatcttcaa cccgatgttc ttcccacagg ccatctgcgg
     6481 cgcgaaaggt acgaatacct gattccgcag aaatccctgc ccctgtcagt acgagtactc
     6541 ttggtttttc cattgcttcc ggcaccactt tatctctgaa aaaaatacgc tgacgcaaac
     6601 gctcgcgcag gcggcgttta tttttacgaa aacgacttaa ccgatgaccc cgacgcgaca
     6661 gcatagcaac ctctgttgtt taatcggtta gatgtaggaa ggccgcacca cgcattcctc
     6721 ccgcatcacc gtggcgcgcg cgttcaatgc gcggaacacg agctacaggt aagagatgac
     6781 gaggcagcct gtccgccagt tgcgttgtga ttgccgggaa attcgataag ccaccaccaa
     6841 tgacgaccag gtcagggtca acaatggtca ggatatttcc cagacaaacc gctaataaat
     6901 ccagataacg ctcaacgtgc gcccttgcct gctcatcgcc ttgatcataa agcgcaataa
     6961 tttcgggagc ctgcaacggt tgatgataat agtgttgata cagccacgca aaaccgcgac
     7021 cagacagata attttcaatg cagccatgct gaccacagcc gcagcggcgt aacgggaaat
     7081 ccaaccccat catggttaac gcatcaaccg gcagacgcat atggccaaac tcgccggtaa
     7141 tgtagctttt accggtaatc ggtttgccgt tgaaaatcag cccgccgcca acgccggtgc
     7201 cgagaatcaa ccccatcacc agtggatatt gagtaaattc atcatcccag gcttctgaaa
     7261 gggcaaaaca gttggcatcg ttatcaaggc gtacatcgcg atcaagacgt gcgctcaggt
     7321 cggcacgcag cggtttaccg ctggcagcgg ggacattggc ggcatacagc gtaccatctt
     7381 ctgtttccgg cattcccgga ataccgatgc cgacagaacc tttacagcca aaacgttggt
     7441 cagcttcagc taccagctca cacactgcat ctaaaaatgc gtcatagctg tcacgcggtg
     7501 tcggcacccg cttttcccac tgcaactgcc gaccgctatc aaacacgcca agcgcaattt
     7561 ttgttccacc aatatcaaac ccgtaataca ttgccgctcc ttgttttaat gtactgcctt
     7621 tactggccgc taaggactcg cgcagggtca atattactgg cgcgccgcgc cggataccaa
     7681 cttgccaaaa gactcagcaa caatgctgtg accagtacgt agaagacgtc cagccagtgc
     7741 aattccgatg gcaggaaatc aataaaatag atatcgctgg agaggaactg atgaccgatc
     7801 aacttttcaa tccactcaat aatcggggta agttgcagtg aaaccaccac accgataatc
     7861 accccacaca ggctgccgaa cagccctgcc agcaatccat accagacaaa gatggcgcga
     7921 attaaaccat ctttcgcccc cagcgttctt aatactgcga tatcgccact cttgtctttc
     7981 accgccatca ctaaggtgga gacgatgttg aaacaggcca cgccaatcac cagtaccatc
     8041 gccagataca taatggcgcg gatcatctgg atatcgcgat acatatagcc gtaagtacca
     8101 atccaacttt taatataaac atagctgttg gtcacttcac ccgcatcgcg taccagctta
     8161 ttggcgttga aaacatccgt cattttaagg gcaatacctg acacgctgga acccatatca
     8221 agatattgtt gggcatccgc cagcgggatc atggcaaaac tgtgatcgag ttgaccactc
     8281 aactgcaaaa taccggcaac gtgcaaacgc acacgttttg gctgcatcag tttatgctcg
     8341 ggattcgagt tggggatcat aatcgacacc caatccccct gcttcacttt cagcgcatcc
     8401 gccacgcctt tgccgatgat aatttgctgt tcgcccgctt taaaattgcg ccacgcatcg
     8461 ccctggacaa acgagggtaa tgcgctcaga cgctgttcct gttgcgggtt aacgcccttc
     8521 acctggattg cgcgaagatt cgcgccactt tccaccagcc cggtgaaatt gatatacggc
     8581 gcagcggcgg caatacccgg cactttttgc acgtgatcca gtgcttcctg ccagttagtc
     8641 cacggctgat ccaccgcctc gatttcacca tgcggcacca ccgccagaat acggttgttc
     8701 agttcgcgtt caaagccgtt catcgcgctt aagccgacga tcaataccgc cacgccgagg
     8761 gcaatgccaa tggtagaaat gacagagatt agcgacacca tgccgccgcg tcgccgtccg
     8821 cgactaaaac gcaggccaat taataacgat aaaggcatcg ccattactcc gcccccatca
     8881 ggctcagttc cgccgtcaga cgaccatcac gcatttctaa ctggcggctc atacgtttcg
     8941 ccagttgcag gtcgtgagta accaccagga aggcggtgcc ctgcaagcga ttcaattccc
     9001 caagcaactg gaagatgctg tcggcgttac gcgcatcgag gttaccggta ggttcatccg
     9061 ccagtaccag gcgcgggtta ttcaccagcg cacgggcaat cgccacacgc tggcgttcgc
     9121 cgccagaaag ttcagatggg cggtgattcg cacgatgctc cagccccacc gcttttaaca
     9181 tctcaagtgc acggctgttg atttcagcgg gctttttctt gccaataagc agcggcatag
     9241 ccacgttttc cagggcggta aaatccggca gcaggtggtg aaactgataa ataaagccca
     9301 gcttctggtt gcgcagctct gctttcgccg ccgaagacag tttgctcatc ggttgaccgt
     9361 taaagatcac atcgccggag gttggcgtat ccagcccgcc caacaagtgc agcaaggtgc
     9421 ttttaccgga accagagcta ccgacgatcg ccatcatttc accttcgccg acgctgaaac
     9481 tgacattgtg cagcacatcg gtttgcacac tgccttcctg atagcgtttg cacaggttgt
     9541 cgcattgcaa caggatctta ttcataacgt aaagcctcag cgggttgagt ggcggcagcg
     9601 cgccatgaag ggtaaagcgt agacagcagc gcgatagcca tcgccaccag cgcaataaca
     9661 atgacctgta aaggttcgat agcgaccggc agcgccgcgc catcaagcag gacgccgatt
     9721 atcggcatca gattatttaa ctggctggca agcagggcgc caagcgccgc tccgaggatc
     9781 gcaccgataa tcccggcgct ggccccttgc accataaaga ccatcatgat ttgtcgcgga
     9841 gttaagcctt gcgtttgcag gatcgctact tcgccctgct tctccattac catcagccct
     9901 agtgaggtaa taatgttaaa cgccgcaacg gcgacaatca ggctcagcag taaacccatc
     9961 atattttttt ccatgcgtac ggcctggaac aactcgcctt tacgatcacg ccagtcctgc
    10021 catttgctgc cttcaggcag tttttgctga cttaatgagt cgactttcag cggctcatcc
    10081 agccacaaac gccagccggt aatattgcct gccggataac gcatcagacg cgaggcatcc
    10141 tcaatattca ccagcatttc attgccatcg acttcactgt tggcggcgaa agtgccaatc
    10201 acattgaaca ggcgctgact tggaatacgc cccatcggcg tgaactggct ggcagatggt
    10261 accatcatgc ggatttgatc accgcgatta acgcctagct gtgaggcaag ttgttcgccg
    10321 aggatgacat tatatttccc cggctcgagg tcagtttgtt tcacattgac cagatacggc
    10381 gttagtggat ctttttgcgc cgggtcgata ccgagcatca ccccgaccgc cacgctgcgc
    10441 gcgctttgca gtaccacatc accggtagta ataggtgcga cgcgattaac gccgtccagt
    10501 ttgactgccg tttccgggag ttgctgcggg ttaagagagc catgctcaga agagagaatt
    10561 gcctgtggca tcaggccaag gatgttgttt tgcagctcgc gctcaaagcc gttcatcact
    10621 gacaatactg tgaccagcgc catcaccccg agggtaatgc cgatggtaga aagccaggag
    10681 acgaaacgac cgaagcgatc cgctgcacgc ccacgcatgt aacgcaggcc aataaataga
    10741 gcgacaggtt ggtacatgaa atccgtctgg ttgctgtagc aaagacacga gtatataagc
    10801 gaaagcaaga tgggtaaacg tctgaactgt aagtgatcgt cagggctaat ctgaaaagcc
    10861 cccgtaaatc aatcggctaa attgccatgt tttatcatca atcttgccgc ctgatcttaa
    10921 tctgaaaaat catccaatac agacagatac ccattacacc tcgggtcata acccgcagga
    10981 tcaaccgcat gtaaattaaa aaagagcgtg ttgtcctgat gaaacaaaaa gagctatgga
    11041 ttaaccagat caaagggtta tgtatttgtc tggtggtgat ttatcactcg gtcattacct
    11101 tttatccgca tctgaccact tttcagcatc cgttatcgga agtcctgagc aaatgctgga
    11161 tctatttcaa tctttacctt gccccctttc gtatgccggt ttttttcttt atttccggct
    11221 atttgattcg ccgctatatc gacagcgtac cgtggggaaa ttgtctcgat aaacggatct
    11281 ggaacatctt ctgggtgctg gcgctttggg gcgtggtgca gtggctggcg ttaagtgcgc
    11341 tgaaccagtg gctggcacct gagcgcgatt taagcaatgc ctccaatgcc gcttatgccg
    11401 attccaccgg tgagttcctg cacgggatga tcaccgccag caccagcttg tggtatctgt
    11461 atgcgttaat tgtctatttc gtggtatgta aaatttttag ccgtctggcg ctgccactat
    11521 tcgccttgtt tgtactgttg agtgtggcgg ttaatttcgt tcccacgccg tggtggggaa
    11581 tgaacagtgt gatccgcaat ttgccttatt acagccttgg cgcatggttt ggcgcaacaa
    11641 taatgacctg tgttaaagag gtgccgttgc gccgccatct gctgatggct tctttgctta
    11701 ccgttctggc ggtcggtgcc tggttgttta ctatctcgct gctgttgtcg ctggtatcga
    11761 ttgtggtgat catgaagctg ttttatcaat acgagcaacg attcggtatg cgctccacca
    11821 gcctgctgaa tgtgattggc tccaacacca ttgctatcta caccacccat cgcattctgg
    11881 ttgaaatatt cagcttaact ctgcttgcgc aaatgaacgc agcacgctgg tcgccgcaag
    11941 tcgaactgac actcctgctg gtttatccct ttgtcagttt gttcatctgt actgttgcgg
    12001 gcttgctggt aagaaaactt tcacagcgtg cattcagcga tctgttgttc accccgcctt
    12061 cactgcccgc tgccgtcagt tactcccgct aagccatcgc cccagccggg gcgattcaat
    12121 ttgctaaacc atgtcgatta cggataatga acagcattgc ttatcagaga tatgccccca
    12181 tatgttgagg catatcctaa cgagaatctg acaaccgtta tgcctgaaca atatcgttat
    12241 acgctgcccg tcaaagcggg tgagcagcgt ctgctgggcg agttaaccgg cgcagcctgt
    12301 gcaacgctgg tagcggaaat tgccgaacgt cacgccggtc cggtggtact cattgcacca
    12361 gatatgcaaa atgctctgcg tttgcatgat gaaatcagtc agttcaccga tcagatggtg
    12421 atgaatctgg cggactggga aactcttccc tacgacagtt tttcgcctca tcaggacatt
    12481 atctcctcgc gcctttccac cctttaccag ctaccgacga tgcagcgtgg cgtactgatt
    12541 gttccggtga atacgcttat gcagcgcgtt tgcccacaca gttttctcca cggtcatgcg
    12601 ctggtgatga aaaaaggtca gcgcctgtca cgagatgcat tacgaaccca actggacagc
    12661 gccggttatc gccatgttga ccaggtgatg gagcacggcg aatacgccac gcgcggcgcg
    12721 ttgctggatc tcttcccgat ggggagtgag ctgccttatc gtcttgattt ctttgatgat
    12781 gaaatcgaca gcctgcgggt gtttgacgtc gacagccagc gcacgctgga ggaagtagaa
    12841 gcgaccaatc tgctgcccgc gcacgaattt ccgaccgata aagcggcaat tgaactgttc
    12901 cgcagccagt ggcgcgatat cttcgaagtg aagcgcgatc cggaacatat ttaccagcaa
    12961 gtgagtaaag gcacattacc tgccgggatc gagtactggc agccattgtt cttcagcgaa
    13021 ccactgccgc cgctgttcag ttatttccct gccaatacct tgctcgtgaa tactggcgat
    13081 ctggaaacca gtgccgaacg tttccagact gacacgctgg cgcgttttga gaatcgcggc
    13141 gtcgatccga tgcgcccgct gttgccacca caatcgctct ggctgcgggt ggacgagctc
    13201 ttctcagagc tgaaaaactg gccccgggtg cagctaaaaa ctgaacattt accgacaaaa
    13261 gccgcgaatg ccaatttagg tttccagaaa ctgccagacc tggccgttca ggcacaacaa
    13321 aaagcgccgc tggatgcgct gcgtaagttc ctcgagactt tcgacggtcc ggtggtgttc
    13381 tcggtagaaa gtgaaggtcg ccgtgaagcg ctgggtgaac tgctcgcacg aattaaaatt
    13441 gctccgcaac gcattatgcg tcttgatgaa gccagcgacc gtgggcgtta tctgatgatt
    13501 ggcgctgccg aacatggttt tgtcgataag gtgcgtaatc tggcgctgat ctgcgaaagc
    13561 gatctgctcg gtgaacgtgt tgcccgccgt cgtcaggatt ctcgccgcac catcaacccc
    13621 gatacactga tccgtaacct tgcggagctg catattggtc agccggtggt ccatctggag
    13681 cacggtgtcg ggcgctatgc cggaatgacc acgctcgaag cgggcggcat tactggcgag
    13741 tatttgatgc tcacctatgc caacgacgcc aaactgtatg ttccggtgtc gtcactgcat
    13801 ctgattagcc gttacgcagg tggcgcggaa gaaaacgccc cgctgcataa acttggcggc
    13861 gatgcgtggt cacgcgcgcg gcagaaagcg gcggaaaaag tgcgtgatgt ggcggcggaa
    13921 ttgctggata tctacgcgca acgcgccgcc aaagagggct tcgcgtttaa acacgatcgt
    13981 gagcagtatc agttgttctg cgacagcttc ccgtttgaaa ccacgccgga tcaggcgcag
    14041 gccattaatg cggtacttag cgacatgtgt cagccgctgg caatggatcg tctggtgtgc
    14101 ggcgatgttg gctttggtaa aacagaagtg gcgatgcgcg ccgctttcct ggcagtagat
    14161 aaccacaagc aggtggcggt gttggtgcct accacccttc tcgcgcagca gcattacgac
    14221 aacttccgcg accgtttcgc caactggccg gtacgtatcg aaatgatctc ccgtttccgc
    14281 agcgccaaag agcagacgca aatccttgcg gaagtggcgg aagggaaaat cgatattctg
    14341 atcggtacgc acaaactgct gcaaagtgac gtcaagttta aagatttagg cctgctgatt
    14401 gtcgatgaag aacaccgctt cggggtgcgt cataaagagc gcattaaagc gatgcgcgcg
    14461 aacgtggata ttctgacgct tactgcaacg ccgatcccac gcacgctgaa tatggcaatg
    14521 agcggaatgc gtgacctgtc gattatcgcc acgccgcccg cccgtcgtct ggcagttaaa
    14581 acctttgtcc gtgagtatga cagcctggtg gtccgggagg cgatcctgcg tgaaattttg
    14641 cgcggggggc aggtttatta tctctacaat gatgtggaaa acatccagaa agctgccgaa
    14701 cggctggcag aactggtgcc tgaagcacgg attgccatcg gtcacgggca gatgcgcgag
    14761 cgcgaactgg aacgggtgat gaatgatttc catcatcaac gtttcaacgt gctggtttgt
    14821 accaccatta tcgaaaccgg gatcgacatc ccgacagcca acaccattat cattgaacgt
    14881 gcggatcact tcggtctggc gcagctgcac cagttacgcg gtcgcgtcgg acgttcgcat
    14941 catcaggcat atgcatggtt gctgacaccg catccaaaag cgatgactac cgatgcacaa
    15001 aaacgtcttg aagcgattgc ctcgctggaa gatctcgggg caggttttgc gctggcaacg
    15061 cacgatctgg agattcgcgg cgcgggtgaa ctgcttggcg aagaacaaag cggctcaatg
    15121 gaaaccatcg gtttctcgct gtatatggag ttgctggaaa acgccgtcga tgcactgaaa
    15181 gccggacgcg agccgtcgct ggaagatctc accagccagc aaacagaagt cgagctgcgg
    15241 atgccgtcgc tattgccaga tgatttcatc cctgacgtga acacgcgtct gtcgttctac
    15301 aaacgtattg ccagcgccaa aacggaaaac gaactggaag agatcaaagt cgagcttatc
    15361 gatcgcttcg gcctgctgcc ggatccggcg cgtaccctgc tggatgttgc ccgtctgcgc
    15421 cagcaagcgc agaaactggg gatcaggaag ctggaaggta atgagaaagg cggggtgatc
    15481 gaatttgccg agaagaatca cgttaatccg gcctggttga ttggtttgct gcaaaaacag
    15541 ccgcagcatt accgccttga tggtccgacg cgcctgaaat ttattcagga tttgagtgag
    15601 cggaaaacgc gtatcgaatg ggtacgccag tttatgcgtg aactggaaga gaacgcgatc
    15661 gcttaatggt aaatcggggg cgtttctgcg cccccatctg caacatttac aaattctttg
    15721 cacttccctg cactatccga cactgtcacc atccataatt caggcttatc tgtttattac
    15781 aataacctta tatttattat ggatttttgg tgatgatcaa aacgcgtttt tctcgctggc
    15841 taacgttttt tacgttcgcc gctgccgtgg cgctggcgct accggcaaaa gccaacacct
    15901 ggccgctgcc gccagcgggc agtcgtctgg ttggcgaaaa caaatttcat gtggtggaaa
    15961 atgacggtgg ttctctggaa gccatcgcca aaaaatataa cgtcggcttt ctcgctctgt
    16021 tacaggctaa ccctggcgtt gatccttacg taccgcgcgc gggtagcgtg ttaacgatcc
    16081 cgttgcaaac cctacttcca gatgcgccgc gcgaaggcat tgtgatcaat attgcggagc
    16141 tgcgtctcta ttactacccg ccgggtaaaa attcggtaac cgtgtatcca ataggtattg
    16201 gtcagttagg tggtgacacg ctgacaccga caatggtgac caccgtttca gacaaacgtg
    16261 caaacccaac ctggacgcca acggcaaaca tccgcgcccg ttataaagca cagggaattg
    16321 agttgcctgc ggtagtgccg gctggaccgg ataacccaat gggccatcat gcgattcgtc
    16381 tggcggccta tggcggcgtt tatttgcttc acggtacgaa cgccgatttc ggcattggca
    16441 tgcgggtaag ttctggctgt attcgtctgc gggatgacga tatcaaaaca ctctttagcc
    16501 aggtcacccc aggcaccaaa gtgaatatca tcaacactcc gataaaagtc tctgccgaac
    16561 caaacggtgc gcgtctggtt gaagtacatc agccgctgtc agagaagatt gatgacgatc
    16621 cgcagctgct gccaattacg ctgaatagcg caatgcaatc atttaaagat gcagcacaaa
    16681 ctgacgctga agtgatgcaa catgtgatgg atgtccgttc cgggatgccg gtggatgtcc
    16741 gccgtcatca agtgagccca caaactctgt aaatcctgga taaaaaaaat ccccgcgaca
    16801 cggcatgtcg cggggaaaaa atcagcagta gcaggcatta atgagggtta atgcttattt
    16861 ataaattact gctgttccat ggagggtatt cggaccggtt acagaagtaa tacggaaaga
    16921 ttttgcgccc atctcatccg ctttttgcgc cagctgctct tccagcgatc ccagatttgt
    16981 ccccgcgtta gcactgattg taccgacttt ttgttggcct tctggcgttg actgaacttc
    17041 gacagccgca aagctggcaa atgacatgga gcttaaaatc gccgcagcga tgagggtttt
    17101 tacgtttttc ataatagtgg ccttatgcag atgaatgacg tcgcaagcaa ttacttaacg
    17161 atcgataact aaatgataga t
//
LOCUS       DAZFXT010000098        17036 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.098, whole genome shotgun sequence.
ACCESSION   DAZFXT010000098 DAZFXT010000000
VERSION     DAZFXT010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17036
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.098"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..886
                     /locus_tag="ACHKW0_003506"
     CDS             <1..886
                     /locus_tag="ACHKW0_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HFO7476283.1"
                     /translation="RTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGKSDSVRDKAS
                     DKDKERVTKNINIMWNALSKNRMFDGNKELKEFVMTLTGSLVFGPNGEITPLSARTTD
                     RSIIRAMMEGGTAKIYHCNDSDKCLKVVADTPVTISRDNALKSQITKLLASIQNKAVS
                     DTPLDDKEKGFISSTTIPVFKYLVDPQMLGVSNSMIYQLTDYIGYDILLQYIQELIQQ
                     ARAMVATGNYDEAVIEHINDNMNDATRQIAAFQSQVQVQQDALLVVDRQMSYMRQQLS
                     ARMLSRYQNNYHFGGSTL"
     gene            883..3704
                     /gene="traG"
                     /locus_tag="ACHKW0_003507"
                     /pseudo
     CDS             883..3704
                     /gene="traG"
                     /locus_tag="ACHKW0_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490585.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating-pair stabilization
                     protein TraG"
     gene            3719..4222
                     /locus_tag="ACHKW0_003508"
     CDS             3719..4222
                     /locus_tag="ACHKW0_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HFO7476284.1"
                     /translation="MIMKNLAHIALVTVIQFIACYLADWGSAETVFILCFIVLWQGLF
                     IWLFSQIRKKRHVSDEFKFSKGVWYILMPVSSLLSPLLSLMVFIIGTLYELRRISGCV
                     SVREWMQSQVNEQTNEDLHLDFDDVNFDYVDFYRTNPATGLAMHGGFDSAGNTFGTCW
                     QDYDDRR"
     gene            4254..4985
                     /gene="traT"
                     /locus_tag="ACHKW0_003509"
     CDS             4254..4985
                     /gene="traT"
                     /locus_tag="ACHKW0_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer complement resistance protein
                     TraT"
                     /protein_id="HFO7476285.1"
                     /translation="MKTKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSSSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            5237..7390
                     /gene="traD"
                     /locus_tag="ACHKW0_003510"
     CDS             5237..7390
                     /gene="traD"
                     /locus_tag="ACHKW0_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001589353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HFO7476286.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILVGLV
                     LWVKISWQTFVNGCIYWWCTTLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLATVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTENPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDLSGEFVKSYYDPSIDKILNPLDAHCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGESFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPDVPEVVSGEDVTQAEQPQQ
                     PVSPAINDKKSDSGVNVPAGGIEQELKMKPEEEMEQQLPPGISESGEVVDMAAYEAWQ
                     QENHPDIQQQMQRREEVNINVHRERGEDVEPGDDF"
     gene            7390..12660
                     /gene="traI"
                     /locus_tag="ACHKW0_003511"
     CDS             7390..12660
                     /gene="traI"
                     /locus_tag="ACHKW0_003511"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HFO7476287.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHA
                     VVANVTQHNGEWKTLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVV
                     GKHGMWEMPGVPVEAFSGRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMTE
                     WMQTLKETGFDIRAYRDAADQRAETRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLNELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKISGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSAKVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQ
                     KAGLHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTSFADLGREINTQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMEKLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNLLPESERPRVVGLGPTHRAV
                     GEIRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVAL
                     SRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPGSDREVMNAERLFSTARELRDV
                     VAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGN
                     GLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGE
                     GRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEI
                     VRKMAENKPDLPDGRTEQAVREIAGQERERAVTSEREAALPESVLREPQREREAVREV
                     VRENLLQERLQQMERDMVRDLQKEKTLGGD"
     gene            12680..13426
                     /gene="traX"
                     /locus_tag="ACHKW0_003512"
     CDS             12680..13426
                     /gene="traX"
                     /locus_tag="ACHKW0_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HFO7476288.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQTWMFLAGRGAFPLFALVWGLNLSRHAHIRQSAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSHRLYRAEDRTERLVLVACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSR
                     VGKSLPRFWPGDFFPTFYGCHLAVLGILAL"
     gene            13485..14345
                     /locus_tag="ACHKW0_003513"
     CDS             13485..14345
                     /locus_tag="ACHKW0_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7476289.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDDESKVFFEKIKARHPEMDIKIPFLTVMETLQYKPAESAA
                     SVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVVA
                     VQTQWFKQYL"
     gene            14448..15008
                     /locus_tag="ACHKW0_003514"
     CDS             14448..15008
                     /locus_tag="ACHKW0_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HFO7476290.1"
                     /translation="MAEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDETVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            15141..15353
                     /locus_tag="ACHKW0_003515"
     CDS             15141..15353
                     /locus_tag="ACHKW0_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HFO7476291.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGSRAVILEVSGRWMEAAEAWRRAAG
                     VAPRTDWQQFARKRAEHCHRRCRGRG"
     gene            15598..16059
                     /locus_tag="ACHKW0_003516"
     CDS             15598..16059
                     /locus_tag="ACHKW0_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HFO7476292.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            16105..16314
                     /gene="hha"
                     /locus_tag="ACHKW0_003517"
     CDS             16105..16314
                     /gene="hha"
                     /locus_tag="ACHKW0_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HFO7476293.1"
                     /translation="MEKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSTSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            16352..16504
                     /locus_tag="ACHKW0_003518"
                     /pseudo
     CDS             16352..16504
                     /locus_tag="ACHKW0_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16505..16911)
                     /locus_tag="ACHKW0_003519"
                     /pseudo
     CDS             complement(16505..16911)
                     /locus_tag="ACHKW0_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611482.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4213 a         3956 c         5047 g         3820 t
ORIGIN      
        1 ccggacgcag gtgtcccagc agaaagtctg tcaggacatt gccggtgaga gcaatatttt
       61 tgctgactgg gcggcttccc ggcagggatg taccgttggc gggaaatctg acagtgtcag
      121 ggataaagcc agcgacaagg ataaggagcg ggtgaccaaa aacatcaaca tcatgtggaa
      181 tgcgctttcc aaaaacagaa tgtttgacgg caacaaagag ctgaaagagt ttgtgatgac
      241 gctgaccggc tcactggtgt ttggtcctaa cggcgaaatc acacccttgt cagccagaac
      301 cactgaccgc tcaattatcc gggccatgat ggaaggcggc acagcaaaaa tttatcactg
      361 caacgattct gataaatgcc tgaaagtggt ggcagacaca ccggtgacca tcagccggga
      421 taatgcactg aagtctcaga ttactaaact tctggccagc attcagaaca aggcggtcag
      481 tgacacgcct ctggatgaca aggaaaaagg ctttatttcc agtaccacca tccccgtctt
      541 caaatacctg gttgacccgc agatgctcgg tgtttccaac agtatgattt accagctgac
      601 ggactatatc ggttacgaca tcctgctgca gtacattcag gagctgatac agcaggcccg
      661 ggcgatggtg gccacaggaa attatgacga agcagttatc gaacatatta acgacaacat
      721 gaatgatgcc acccggcaga ttgcggcgtt tcagtcacag gtgcaggtac agcaggatgc
      781 gctgctggtt gtcgatcgtc agatgagcta catgcgtcag cagctttccg cccgcatgct
      841 cagtcgttac cagaacaact atcacttcgg agggagcacg ctgtgaatga agtttatgtg
      901 attgccggtg gtgagtggtt gcggaataac ctgaacgcca ttgccgcctt tatgggaacc
      961 aggacgtggg attccattga aaaaattgcg ctcacattgt ctgttctcgc ggtggccgtg
     1021 atgtgggtac agcggcacaa cgtgatggat ttgctgggct gggtggccgt gtttgtgctt
     1081 atcagcctgc tggttaatgt ccgcacatcg gtgcagatta ttgataacag tgacctggtc
     1141 aaagttcacc gggtggataa tgtaccggtc ggactggcga tgccactttc actgacgacc
     1201 cgtatcgggc atgcaatggt ggccagttac gagatgatct tcacgcaacc ggacagtgtc
     1261 acctatagca aaacggggat gctgtttggc gcagaactgg tatcaaaaag caccgacttt
     1321 ttgtcccgca atccggaaat cgccaatctt ttccaggatt atgtgcagaa ctgcgtgatg
     1381 ggggatattt acctgaacac aaatacacgc tggaggagct tatggcctcc gctgatccct
     1441 acacgctgat tttttcccgc cccagtccgc tacggggcgt ttatgacagc aataataatt
     1501 tcgtgacctg taaggacgcg tcggtttcgc tgaaagacaa gctgaatctg gatacacaga
     1561 gtggcggaaa aacctggcat tattatgccc agcagctatt tggtggacgc cctgatccga
     1621 acctgttgtt cagtacgctg attggcgaca gttacagtta cttctatggc tcaagtaaat
     1681 cagccagcca gatcatccgc cagaatgtca ccatcaatgc gctgaaggag gggatcacca
     1741 gttatgccgc gcgtaatggt gattcagcca gcctggtgaa tctggccacc acgtcatcga
     1801 tggagaagca acgtctggca catgtctcca tcggtcatgt ggcgatgcgg acactgccca
     1861 tgacgcagac catcctgacg gggatcgcca tcggtatttt cccgcttctg gtactggcgg
     1921 ctgtcttcaa caaactgacg ctgtccgtcc tgaaaggcta tgtgtttgcc ctgatgtggt
     1981 tgcagtcatg gccaatgttg tacgccattc tgaacagcgc catgacattt tatgcgaagc
     2041 agaatggtgc accggtggtg ctctcggaaa tttcacagat acagctgaaa tactccgatc
     2101 tggcttccac tgccgggtat ctttccatga tgatcccgcc attatcctgg atgatggtca
     2161 ggggactcgg ggccggattt tccagtgtgt acagccattt cgcttcctct gctatcagcc
     2221 cgactgccag tgcggcaggt agtgtggttg acggtaatta ctcctacggc aacatgcaga
     2281 cggaaaacgt gaacggattc agctggagca ccaacagcac cacgtcgttt ggtcagatga
     2341 tgtaccagac cggcagtggc gcaaccgcta cacagacccg tgacggtaat atggtgatgg
     2401 atgcgagcgg ggcgatgtcc cgtttaccgg tcggtatcaa tgcaacgcgt cagattgcgg
     2461 cggcacaaca ggaaatggcc cgggaggcgt cgaacagagc agaaagcgcc ctgcatgggt
     2521 tcagcagcag tattgccagt gcctggaact cgctcagtca gtttggttct aaccggggga
     2581 gcagtgattc tgtcaccggt ggtgctgaca gcacgatgag cgcacaggac tccatgatgg
     2641 ccagccgtat gcgcagcgca gtggaaagct atgcgaaggc gcataatatc agtaatgagc
     2701 aggcgacaag agaactggca tcaagaagta cgagaggttc tgcggggatg tatggcgatg
     2761 ctcatgctga atggggggtt aaacctaaaa ttcttggtgt tggtggtggc gtaggtgtta
     2821 gaggtggtgg tcgggcaagt attgactgga gtgatgagga tgcgcatcag gccagcagtg
     2881 gttcacgggc cagccatgat gctcgccatg atatcgatgc cagggctaca caagacttta
     2941 aagaggccag cgattacttt accagtcgca aggtcagtga atccggcagc catacggaca
     3001 ataatgccga ttcccgtgtg gaccagctgt ctgcagccct gaactcagca aaacagagtt
     3061 acgaccagta cacgacgaat atgacccgca gccatgaata tgctgaaatg gcatcccgta
     3121 ctgaaagcat gagtgggcag atgagtgaag acctgtcgca acagtttgca cagtatgtga
     3181 tgaaacacgc gccgcaggat gccgaagcta ttctgacaaa caccagttca ccggagattg
     3241 cagaacgccg tcgggctatg gcgtggtctt ttgttcagga acaggtgcag cctggtgttg
     3301 ataatgcctg gcgtgaatcc cgtggggaca tcggtaaagg aatggagagc gtaccttcgg
     3361 ggggtggcag ccaggatatt attgctgatc atcaggggca tcaggccatt attgagcaaa
     3421 gaacgcagga cagtaatatc cgtaatgatg taaaacatca ggttgataat atggtcacag
     3481 aataccgagg caatatcgga gatactcaga gcagcatacg tggcgaagaa aatattgtag
     3541 gaagacagta ttctgaacta caaaatcacc atataacata ggcattatct cagaataata
     3601 aatataatga agaaaaatcg ggtcaggaaa gaatgcccgg ggctgacagt ccacaggaac
     3661 tgatgaaaag agcaaaggag tatcaggata agcacaagca ataatattgt atggggagat
     3721 gataatgaaa aatttagctc atattgctct ggtgacggtg atacagttca ttgcctgtta
     3781 tctggctgat tgggggagtg ccgaaacagt ttttatcctg tgctttattg tactatggca
     3841 gggattgttt atatggttat tttctcaaat acgaaaaaaa cggcatgtgt cggatgagtt
     3901 taaattcagt aaaggcgttt ggtatatact gatgccagta tcttccttac tctctccgtt
     3961 attaagcctg atggttttta tcattggtac actatacgaa ttaaggcgaa ttagtggttg
     4021 tgtaagtgta agagaatgga tgcaatctca ggtaaatgaa caaactaatg aagatttgca
     4081 tctggatttt gatgatgtga attttgatta tgtggatttt tatcgtacta atccggcaac
     4141 aggattagct atgcatggag gatttgattc agcaggaaat acttttggga cttgttggca
     4201 ggactatgat gacaggcgat aagaaaactg tggtttgcaa taaaggtaat ttgatgaaaa
     4261 caaaaaaatt gatgatggtt gcactggtca gttccactct ggccctttca gggtgtggtg
     4321 cgatgagcac agcaatcaag aagcgtaacc ttgaggtgaa gactcagatg agtgagacca
     4381 tctggcttga acccgccagc gaacgcacgg tatttctgca gatcaaaaac acgtctgata
     4441 aagacatgag tgggctgcag ggcaaaattg ctgatgctgt gaaagcaaaa ggatatcagg
     4501 tggtgacttc tccggataaa gcctactact ggattcaggc gaatgtgctg aaggccgata
     4561 agatggatct gcgggagtct cagggatggc tgaaccgtgg ttatgagggc gcagcagttg
     4621 gtgcagcgtt aggtgccggt atcaccggat ataattccag ttctgccggt gccacactcg
     4681 gtgtgggcct tgctgccggt ctggtgggta tggctgcaga tgcgatggtg gaagatgtga
     4741 actataccat gatcacggat gtgcagattg cagagcgtac taaggcaacg gtgacaacgg
     4801 ataatgttgc cgccctgcgt cagggcacat caggtgcgaa aattcagacc agtactgaaa
     4861 caggtaacca gcataaatac cagacccgtg tggtctcaaa tgcgaacaag gttaacctga
     4921 aatttgaaga ggcgaagcct gttctcgaag accagctggc caaatcaatc gcaaatattc
     4981 tctgattatc ttccggaggc tggtctgacc ggcctcctga ctgacgcctc gctttgctcg
     5041 ttgtccaacc ccggaaagaa ataaaataaa ttttatttct ccccggcgtt tccgaaataa
     5101 atatgaaaac actgagcggt tgtgcaaatt ctttgtgcga ccgtgaagtg ttgccggcga
     5161 agccggaatt taacggttat taatcttttc atcattaata atcagtccga ctgacttttt
     5221 tcttcggaat atcatcatga gttttaacgc aaaggatatg acccagggcg gtcagattgc
     5281 gtccatgcgt atccgcatgt tcagccagat cgccaatatc atgctttact gcctgtttat
     5341 ttttttctgg atactcgttg gtctggtttt atgggtaaaa ataagctggc agacgtttgt
     5401 gaacggctgt atttactggt ggtgtaccac gctggaaggc atgcgggatt taatcaagtc
     5461 ccagccggta tatgagatcc agtattacgg caaaaccttc cggatgaacg ctgctcaggt
     5521 actgcatgat aaatatatga tctggtgcgg agagcagcta tggtccgcat tcgttctggc
     5581 tacagttgtg gcactggtta tttgcctgat taccttcttt gttgtctcct ggattctggg
     5641 gcgtcagggt aaacaacaga gcgaaaatga agtcacaggt ggtcgtcagc tgacggagaa
     5701 cccgaaagac gttgcccgga tgctgaaaaa agacggcaag gactccgata tccggattgg
     5761 cgacctgccg attatccggg attccgaaat ccagaacttc tgtctgcacg gcacggtcgg
     5821 ggccggtaag tcggaagtta tccgtcgtct ggccaactac gcccgtcagc gtggagatat
     5881 ggtggtgatt tatgaccttt caggggaatt tgttaaaagt tactatgatc cctccatcga
     5941 taaaatcctg aatccgctgg atgcccactg tgccgcctgg gatttatgga aggagtgtct
     6001 gacacagccg gattttgata atacggcaaa taccctgatc ccgatgggca caaaagagga
     6061 cccgttctgg cagggttcag gacgtaccat ttttgcggaa gcggcgtacc tgatgcgtaa
     6121 tgaccccaac cgcagctaca gcaaactggt ggacacactg ctttccatca aaatcgaaaa
     6181 actgcgtacc ttcctgcgta attcaccggc ggccaacctg gtggaagaga aaattgagaa
     6241 aacggcgatt tccatccgtg ctgtgctgac caactacgtg aaagccatcc gttacctgca
     6301 ggggattgag cataacggtg agtcttttac catccgtgac tggatgcggg gtgtccggga
     6361 agatcagaaa aacggctggc tgtttatctc gtcgaatgcc gacacccatg cctccctgaa
     6421 gccggtgatc tccatgtggc tgtccattgc cattcgtggt ctgctggcaa tgggagaaaa
     6481 ccgtaaccgt cgtgtgtggt ttttctgtga tgagctaccc acgttacaca aactgccgga
     6541 cctggtggag atcctgccgg aagcccgtaa gttcggtggc tgttatgtgt ttggtatcca
     6601 gtcctatgcc cagctggaag atatctacgg tgagaaagcg gctgccacgc tgtttgacgt
     6661 catgaacacc cgtgcctttt tccgttctcc cagccataag attgcagagt tcgctgccgg
     6721 tgagattggt gagaaagagc acctgaaagc cagcgagcag tactcctacg gtgctgatcc
     6781 ggtacgtgac ggggtatcga ccggtaagga tatggagcgc cagacgctgg tcagttattc
     6841 cgacattcag tctctgccgg atttgacctg ttatgtcacc ctgcccggac cgtatccggc
     6901 agtaaaactc tcactgaaat atcaggcacg tccgaaggtt gcccctgagt ttattccgcg
     6961 tgacatcaac ccggaaatgg agaaccgcct gagcgccgta cttgccgcaa gggaagcaga
     7021 aggtcgtcag atggccagcc tcttcgaacc ggatgtgccg gaagttgttt ccggagaaga
     7081 cgtgactcag gctgaacagc cgcaacagcc ggtgtctcct gccatcaacg ataagaagtc
     7141 agattcaggt gtgaatgttc cggcaggggg gatcgagcag gagctgaaaa tgaaaccgga
     7201 agaagagatg gaacagcaac tgccacccgg gatcagtgaa tccggtgaag tggtggatat
     7261 ggccgcttat gaggcatggc aacaggaaaa tcatccggac atccagcagc agatgcagcg
     7321 tcgtgaagag gtgaacatta atgtgcaccg ggagcgcggg gaggatgttg agccgggaga
     7381 tgatttctga tgatgagtat cgcgcaggtc agatcggccg gaagtgccgg taactattat
     7441 accgacaagg ataattacta tgtgctgggc agcatgggag aacgctgggc cggcaggggg
     7501 gctgaacagc tgggactgca gggcagtgtc gataaggatg tttttacccg tcttctggag
     7561 ggaaggctgc cggacggagc ggatctaagc cgcatgcagg atggcagtaa caggcatcgt
     7621 cccggctacg acctgacctt ctccgctccc aaaagtgtct ccatgatggc catgttaggt
     7681 ggcgataagc gcctgattga tgcacataac caggccgtgg actttgctgt tcgtcaggtg
     7741 gaggcgctgg cctccacacg ggtgatgacg gacggacagt cagaaacggt gctgaccggt
     7801 aatctggtga tggcactgtt taaccacgac accagtcgcg atcaggaacc acagttacac
     7861 acgcatgcgg tggtggctaa tgtcacgcag cataacggcg aatggaagac actgagcagt
     7921 gacaaagtgg ggaaaacggg gttcattgag aatgtgtacg ctaatcagat tgcctttggc
     7981 aggctctacc gggaaaaact gaaagagcag gttgaggcgc tgggctatga aaccgaagtg
     8041 gtgggtaagc acggtatgtg ggagatgccg ggcgtaccgg tggaggcctt ttccggacgc
     8101 tcacaggcaa tccgggaggc cgtcggggaa gatgcctcgc tgaaatcccg ggatgtggcg
     8161 gcactggata cgcgaaaatc caaacagcac gtcgatcctg aagtcagaat gactgaatgg
     8221 atgcagacgc tgaaggaaac cgggttcgac atccgggcat atcgtgacgc ggcggatcag
     8281 cgtgcggaga cccgcacgca ggcgcccggg gctgtttcac aggaggggcc ggatgtgcag
     8341 caggcggtga cacaggccat tgccggatta agtgaacgaa aagtgcagtt tacgtacacg
     8401 gacgtgctgg ccagaacggt cggcatactg ccgccggaaa atggtgtgat tgaacgggca
     8461 cgcgccggta tcgatgaggc catcagccgt gagcagctta tccccctcga tcgtgagaag
     8521 gggctgttca cgtccgggat tcatgtgctc aatgagctgt cagtccgggc actcagtcgt
     8581 gacatcatga aacagaaccg ggtgaccgta catccggaga aaagtgtccc ccggacggct
     8641 ggttacagcg atgcggtcag tgttctggca caggaccgtc cgtcgctggc cattgtgtcc
     8701 gggcagggcg gtgcagccgg gcagcgtgag cgggtggctg aactggtcat gatggcccgg
     8761 gagcaggggc gggaggtgca gattatcgct gctgaccgtc gctcgcagat gaacctgaag
     8821 caggatgaac ggttgtccgg tgagctgata accggacgtc gtcagctgct ggaaggcatg
     8881 gccttcacgc cgggcagtac cgttatcgtt gaccagggcg aaaaactctc cctgaaagag
     8941 acgttaaccc tgctggacgg tgccgcacgt cataacgtac aggtcctgat aaccgacagc
     9001 gggcagcgaa ccggtacagg cagtgcgctg atggccatga aggatgccgg ggtgaacaca
     9061 tatcgctggc aggggggaga acagcgaccg gccaccatca tcagtgaacc ggaccgtaat
     9121 gtccgctatg cccggctggc cggagatttt gcggccagcg tgaaagccgg agaagagagc
     9181 gtggcacagg tcagcggagt acgggaacag gccatactga cacaggccat tcgcagtgag
     9241 ctgaaaacac agggcgtgct cggacaccag gaggtgacca tgacggcact gtcaccggtc
     9301 tggctggaca gccggagccg ttatctgcgg gatatgtacc gtccggggat ggtgatggag
     9361 cagtggaacc cggagacacg cagtcatgac cgctatgtta tcgaccgggt gacggcgcag
     9421 agtcacagcc tgaccctgcg ggatgcgcag ggtgaaacgc aggtggtgcg tatttcctcc
     9481 ctggacagca gctggtcgct gttccggccg gaaaaaatgc cggtggcaga cggcgagcga
     9541 ctgagggtga cagggaaaat ttccggactc cgcgtctccg gcggtgaccg cctgcaggtg
     9601 gcatccgtca gtgaagatgc gatgacggtt gttgtaccgg ggcgggctga accggcctcc
     9661 ctgcctgtga gcgattcacc attcacggca ctgaagctgg agaacggctg ggtggaaacg
     9721 cccgggcatt ccgtcagcga cagtgcgaag gtttttgcct ccgtcacaca gatggcgatg
     9781 gacaacgcga ccctgaacgg tctggcccgc agtggtcgtg atgtccggct gtattcctca
     9841 ctggatgaaa cccgtactgc ggaaaaactt gcccgccatc cctcctttac ggtggtttct
     9901 gagcagataa aggcgcgtgc cggtgagaca ttgctggaaa ccgctatcag tctgcagaaa
     9961 gccgggcttc acacgccggc acagcaggcc attcatcttg cccttccggt ggtggaaagt
    10021 aaaaacctgg ccttcagcat ggtggacctg ctgacagagg caaagtcgtt tgctgcagaa
    10081 ggaaccagtt ttgctgacct gggacgggaa atcaatacgc agataaaacg cggtgattta
    10141 ctgtatgtgg atgtggcaaa aggctatggc acaggcctgc tggtttcccg tgcgtcgtat
    10201 gaggcagaaa agagcattct tcgccatatt ctcgaaggta aggaggcggt cacgccgctg
    10261 atggagagag tacctggcga actcatggag aaactgacat caggacagcg tgccgccacc
    10321 cgcatgatac tggaaacgtc cgaccgtttc acggtggtgc agggctatgc cggtgtgggt
    10381 aagaccacac agttccgggc ggtgatgtca gccgtgaacc tgctgccgga gagtgagcgt
    10441 ccccgtgtcg ttgggctggg tcccacacac cgtgcggtcg gggagatacg cagcgccggc
    10501 gtggatgcgc agacactggc gtcctttctg catgacacgc agctgcagca gcgcagcgga
    10561 gaaacgccgg atttcagcaa cacgctgttc ctgctcgatg agagctcaat ggtgggcaat
    10621 accgacatgg cacgggcata cgccctgatt gcggccggtg gcggtcgtgc cgtggccagt
    10681 ggtgacacgg accagctgca ggccatcgcg cccggtcagc ctttccgtct ccagcagacg
    10741 cgcagtgctg ccgatgtggc tatcatgaaa gagattgtgc gtcagacgcc ggaactgcgg
    10801 gaggcggtat acagcctgat taaccgggat gtggaaaggg cattgtccgg gcttgagagt
    10861 gtgaaaccgt ctcaggtgcc tcgtcaggag ggcgcatggg caccggagca ctctgtgacg
    10921 gagttcagtc acagccagga agcgaaactg gcagaagcgc agcagaaggc gatgctgaaa
    10981 ggcgaggctt tcccggatgt ccccatgaca ctgtatgaag ccattgtccg cgactatacc
    11041 ggcaggacac cggaagcacg ggagcagacg ctgattgtca cacacctgaa tgaggaccgg
    11101 cgcgtactga acagcatgat tcatgatgca cgggaaaagg ccggtgagct ggggaaagag
    11161 caggtcatgg tgcctgtcct gaacacagcg aatatacgtg acggagaact gcgtcgtctc
    11221 tccacctggg agaataaccc ggatgccctt gccctggtgg atagtgtgta tcaccggatt
    11281 gccggtatca gcaaggatga cgggctgata accctggagg atgcggaagg taacacgcgg
    11341 ctgatttcgc cccgggaggc ggtggctgaa ggcgtcacac tgtacacccc ggacaccatc
    11401 cgggtgggaa ccggtgaccg gatgcgcttc acgaagagtg accgggagcg cggttatgtg
    11461 gccaacagcg tctggacggt gacagcggtt tccggtgaca gtgtcacgct gtcggacgga
    11521 cagcagaccc gggtgattcg ccccggtcag gagcgggcag agcaacatat tgacctggcc
    11581 tatgccatca ccgcccacgg tgcgcagggg gcaagtgaaa cctttgccat tgcgcttgaa
    11641 ggcacggaag gcgggcggaa acagatggcc ggctttgagt cggcctacgt ggccctgtcg
    11701 cgtatgaagc agcatgtgca ggtgtacacc gataaccgtc agggctggac ggatgccatc
    11761 aacaatgccg tacagaaagg aactgcccac gatgtgctgg agccggggtc tgaccgggag
    11821 gtcatgaatg cagagcggct gttcagtacg gcgcgggagc tgcgggacgt ggtggccggc
    11881 cgtgccgttc tccgtcaggc agggctggcc gggggagaca gtcctgcacg gtttattgca
    11941 cccgggcgca aatatccgca gccgtatgtg gcactgccgg cttttgaccg taacggcaag
    12001 tcagctggta tctggctgaa cccgctgacc acggatgacg gaaacgggct gcggggcttc
    12061 agtggtgaag gccgggtgaa aggcagcggg gatgcgcagt tcgtggccct gcagggcagc
    12121 cgtaacggag aaagcctgct ggctgataat atgcaggatg gtgtccggat tgcccgtgat
    12181 aatcctgaca gcggtgtggt ggtgagaatt gccggtgaag gtcgtccgtg gaatcccggt
    12241 gccatcaccg gtggtcgcgt gtggggggat attccggata acagcgttca gccgggtgcc
    12301 ggaaacggtg aaccggtcac ggcagaggta ctggcacagc ggcaggctga agaggccatc
    12361 cgccgtgaaa cggaacgccg cgcagatgaa attgtccgta aaatggcaga gaacaaacct
    12421 gacctgccgg acggcagaac agagcaggct gtcagggaga ttgccgggca ggagcgtgaa
    12481 cgggctgtca cttctgaacg ggaagccgcg ctgccggaga gtgtactgcg tgaaccacaa
    12541 cgggagcggg aggcggtccg tgaggttgtc cgggaaaacc tgctgcagga gcgactgcag
    12601 cagatggagc gggatatggt tcgtgacctg cagaaagaga aaaccctggg cggagactga
    12661 tacaggaaga taaacgctga tgacaacgga taacacgaac acgacacgta acgattcact
    12721 ggctgcccgg accgatacct ggttgcagtc gttgctggtc tggtcacccg gacagcggga
    12781 tatcatcaaa acggtggcac tggtgctgat ggtactggac catgcaaacc gcattctgca
    12841 tctggaccag acgtggatgt ttctggccgg gcgtggagcc tttccgttgt ttgctctggt
    12901 gtgggggctg aatctgtccc gccatgcgca tatcaggcag tcggccatta accggctgtg
    12961 gggatgggga attattgccc agttcgcgta ttatctggca ggctttccgt ggtatgaggg
    13021 gaatatcctg tttgcctttg cagtggcggc acaggtgctg acgtggtgtg aaacgcgcag
    13081 tgggtggcgt acagcagcgg ccattctcct gatggcactg tgggggcctt tgtccggcac
    13141 cagttacggc attgccgggc tgctgatgct ggcagtcagt caccggctgt accgggcgga
    13201 agacagaacg gaacgtctgg tgctggttgc atgcctgctg gccgtgattc cggcacttaa
    13261 ccttgcctcc agtgatgcgg cggctgtagc tggtcttgtg atgacggtgc tgaccgttgg
    13321 tctggtgtcg cgtgtaggaa aatcattacc ccgtttctgg cccggagatt ttttcccgac
    13381 gttctatggc tgtcatctgg ctgtgctggg cattctggcg ttgtgactgg tatgatactg
    13441 acgctgattc tgcttattaa taaacaacat aacacaggat atttatgaaa attacggatc
    13501 acaaactctc tgaaggtatt gccctgacat ttcgtgttcc tgaaggaaat ataaaacatc
    13561 ctttgattat cttatgccat gggttttgcg gtatcaggaa tgttctgctc ccttgctttg
    13621 cgaatgcctt cacagaagca ggattcgcaa cgattacttt tgattatcga ggattcggtg
    13681 agagtgatgg agaacgaggg cgtctggttc ctgcaatgca gaccgaagat attatttcag
    13741 ttatcaattg ggcagaaaag caggaatgta ttgataatca gcgtattggg ttatggggaa
    13801 catcgctggg aggggggcat gtcttcagtg cagctgcgca ggaccaacgc gtaaaatgta
    13861 ttgtcagcca gctggctttt gctgacggtg atgttctggt taccggcgag atgaatgaat
    13921 cggagagagc gtcttttcta tccacgctga ataaaatggc agagaagaag aaaaacaccg
    13981 ggaaagagat gtttgtgggg gttacacggg tactaagtga tgatgaatcg aaggtattct
    14041 tcgagaaaat caaagcccgg catcccgaaa tggatataaa aatacctttc cttacggtca
    14101 tggaaacgtt gcaatataaa ccggcagaga gtgcagccag cgtccagtgc ccagttttgg
    14161 ttgttattgc cggccaggat agcgttaatc caccggaaca gggaagagca ttatatgatg
    14221 ctgttgcttc tggtactaag gaattatacg aggaagctga tgcatgccat tatgatattt
    14281 atgagggcgc gttttttgaa cgtgtagtag ctgtgcagac acaatggttt aaacaatatc
    14341 tttaattgtc agtatttata cgttaaaggt cgttgaaatt aacttcctgt tacgatgagt
    14401 tgtttcaacg gcctgcaggg catattttca cgaaggtcag agtaagcatg gcagagcaaa
    14461 aacgaccggt actgacactg aagcggaaaa cggaaggaga gacgcctgtc cgcagccgga
    14521 aaaccatcat caatgtcacc acgccaccaa aatggaaggt gaaaaagcag aagctggccg
    14581 agaaagcagc ccgggaagcg gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
    14641 ccatttatct gaacctgccc acgctggatg agaccgtgaa caccctgaaa ccctggtggc
    14701 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
    14761 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaagctgcgc agggcgctga
    14821 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
    14881 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcggcagcgc
    14941 gtctggataa aatccgccgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
    15001 agaaataaaa aaagcctccc tcagagagag gaggcaggga aataaggctt aaaaggaaat
    15061 aacttccgta cccgaaagga agctaattca gtagattatt taacaccata atactgacgg
    15121 gttaaagagg agtgataaag atgaatggat tcagaaacag ttcacggaac ggtcaggtct
    15181 ggcgttacca gcgtgccgga agccgggccg ttattctgga agtcagtgga cgctggatgg
    15241 aagcggcaga agcatggcga cgggctgccg gtgttgcacc ccggacagac tggcagcagt
    15301 ttgcccgaaa aagggctgaa cactgccacc ggcgctgccg aggaaggggg taacgaaaaa
    15361 agcagccttc attactccag ggattcagaa ggctgaataa tgcatgagat ttgctttttt
    15421 taatatccgg tcgggcagta tgaccggaca gttctgagta gcacagagtt ttttccgtat
    15481 taagtcgtga ttttatattt tgtgattaat ttcacaaaat aaggtgttat tcagtgtgtg
    15541 ctgcaatatt caggatgcct gaaatacagg tgctatttat ctgatacgga gaataacatg
    15601 aggaaatata ttccactggt attatttatc ttttcatggc cggtattgtg tgcagatatt
    15661 catggacggg ttgttcgtgt actggacggt gataccatcg aggtcatgga ctcacggaag
    15721 gcggtgcgga tcaggctggt aaatattgat gcgccggaaa agaaacagga ttatggccgg
    15781 tggtcgacag acatgatgaa atcactggtt gcaggaaaaa cggttaccgt cacatatttt
    15841 cagcgagatc gttatggtcg tattctcggg caggtttatg caccagacgg aatgaatgtt
    15901 aatcagttta tggtccgggc cggggccgcc tgggtatatg agcagtacaa cacagatccc
    15961 gtgctgcctg tccttcagaa cgaagccaga caacaaaaac gtgggctctg gtcagatgct
    16021 gatccggttc ccccctggat atggagacac cgtaaataaa gcgatggcaa tcttatcatt
    16081 catctgttct cagcgggaat aaaaatggaa aaaacaaaac aggaatggct gtatcaatta
    16141 cggcgatgtt catcagtcaa tacgctggaa aaaattattc ataaaaacag agacagttta
    16201 tcgaccagtg aacgtgaatc atttaattca gcagcagacc accggttagc agaattaatc
    16261 accggaaagt tatatgacag gataccaaaa gaaatatgga aatatgtaag gtaataattc
    16321 agcataatta attaccagga gcagataaac aatgaagctg attattttta ttctcattgt
    16381 tctgattatt gcagcgttac ttatccggat tatcctcaga agtgtaaatc agcattctcc
    16441 tttgctgatg caattgcatg cggcaggtat acggaccggt gatgcagagc gaatactgtc
    16501 tgccgtctgg tttttcagga cggtttttcc agtatctgta gctgctgcga tgaaccccga
    16561 acacatggca gagtgtgacc acaggataat gcgctctgag tttcccgatt atcgagaact
    16621 gttcagggag tctgacatca agagcgcggt agcctttttt aatatttcat tctccatttc
    16681 aatgcgttgt agctttttcc tcagctcacg tatttcgatt tgttctggtg ttatcggaga
    16741 ggcttttggt gttttgccct gacgctcatc acgcagttgt ttgacccatc ttgtcattgt
    16801 ggaaaggccg atatccatag ctttggcggc atctgccacc gtgtagttct ggtcaacaac
    16861 cagttgagcg gattcgcgtt taaactctgc gctgaaattt ctttttttca ttggagcacc
    16921 tgtgttgttc tgaggtgagc atatcacctc tgttcaggtg gccaaattca gtgtgccact
    16981 acatctttcg aaaaccagtt ctgcggcttt tcaaagatgc catcatctct cttcac
//
LOCUS       DAZFXT010000099        16912 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.099, whole genome shotgun sequence.
ACCESSION   DAZFXT010000099 DAZFXT010000000
VERSION     DAZFXT010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.099"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            152..694
                     /gene="ecpR"
                     /locus_tag="ACHKW0_003520"
                     /gene_synonym="matA"
     CDS             152..694
                     /gene="ecpR"
                     /locus_tag="ACHKW0_003520"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HFO7476294.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLTEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMLPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            769..1356
                     /gene="ecpA"
                     /locus_tag="ACHKW0_003521"
                     /gene_synonym="matB"
     CDS             769..1356
                     /gene="ecpA"
                     /locus_tag="ACHKW0_003521"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HFO7476295.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATRTS"
     gene            1418..2086
                     /gene="ecpB"
                     /locus_tag="ACHKW0_003522"
                     /gene_synonym="yagY"
     CDS             1418..2086
                     /gene="ecpB"
                     /locus_tag="ACHKW0_003522"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HFO7476296.1"
                     /translation="MKKHLLLLALLLSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVIAMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2112..4637
                     /gene="ecpC"
                     /locus_tag="ACHKW0_003523"
                     /gene_synonym="yagX"
     CDS             2112..4637
                     /gene="ecpC"
                     /locus_tag="ACHKW0_003523"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HFO7476297.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDHGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNFGIYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSNIASI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGPFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYVNTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4627..6270
                     /gene="ecpD"
                     /locus_tag="ACHKW0_003524"
     CDS             4627..6270
                     /gene="ecpD"
                     /locus_tag="ACHKW0_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HFO7476298.1"
                     /translation="MRVNLLIAMIIFALIWPATALRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYNGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQASGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSRSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSQESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6239..6949
                     /gene="ecpE"
                     /locus_tag="ACHKW0_003525"
     CDS             6239..6949
                     /gene="ecpE"
                     /locus_tag="ACHKW0_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HFO7476299.1"
                     /translation="MFRRRGVTLTKALLTAVCMLATPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPECDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7262..7591
                     /gene="ykgJ"
                     /locus_tag="ACHKW0_003526"
     CDS             7262..7591
                     /gene="ykgJ"
                     /locus_tag="ACHKW0_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HFO7476300.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7839..8451)
                     /gene="yagU"
                     /locus_tag="ACHKW0_003527"
                     /pseudo
     CDS             complement(7839..8451)
                     /gene="yagU"
                     /locus_tag="ACHKW0_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
     gene            8869..9558
                     /gene="paoA"
                     /locus_tag="ACHKW0_003528"
     CDS             8869..9558
                     /gene="paoA"
                     /locus_tag="ACHKW0_003528"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0047770 - carboxylate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit PaoA"
                     /protein_id="HFO7476301.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAALKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9555..10511
                     /gene="paoB"
                     /locus_tag="ACHKW0_003529"
     CDS             9555..10511
                     /gene="paoB"
                     /locus_tag="ACHKW0_003529"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HFO7476302.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDALFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10508..12706
                     /gene="paoC"
                     /locus_tag="ACHKW0_003530"
     CDS             10508..12706
                     /gene="paoC"
                     /locus_tag="ACHKW0_003530"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HFO7476303.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12716..13672
                     /gene="paoD"
                     /locus_tag="ACHKW0_003531"
     CDS             12716..13672
                     /gene="paoD"
                     /locus_tag="ACHKW0_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HFO7476304.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHLTILRFAVEALMSGKGAVLV
                     TLVEIRGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLTLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLETQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPVLQAAREAKPFYLGALGSYRTHTLRLQKLHELGWSREETTQ
                     IRAPVGIFPKARDAHTLALSVLAEVASVRLHQEEDSCLPPSS"
     gene            13723..14061
                     /locus_tag="ACHKW0_003532"
                     /pseudo
     CDS             13723..14061
                     /locus_tag="ACHKW0_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
     gene            complement(14188..14263)
                     /locus_tag="ACHKW0_003533"
     tRNA            complement(14188..14263)
                     /locus_tag="ACHKW0_003533"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14228..14230),aa:Thr,seq:cgt)
     gene            complement(14378..15631)
                     /gene="proA"
                     /locus_tag="ACHKW0_003534"
     CDS             complement(14378..15631)
                     /gene="proA"
                     /locus_tag="ACHKW0_003534"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HFO7476305.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            complement(15643..16746)
                     /gene="proB"
                     /locus_tag="ACHKW0_003535"
     CDS             complement(15643..16746)
                     /gene="proB"
                     /locus_tag="ACHKW0_003535"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HFO7476306.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
BASE COUNT         4136 a         4558 c         4467 g         3751 t
ORIGIN      
        1 ttaatctcgt ttaacctata ttgatatgtg ctacgtatct tatttacttc cgatttacta
       61 aagaaactga atgtacctgt aaaaattaca ggtttggaaa gtagtgacat ggcaaaatga
      121 ttacagcagg gactatgagg ttaaaaacca tatggaatgt caaaaccgtt ctgataaata
      181 catctggtct ccccatgacg cctacttcta taaaggacta tctgaactga ttgtggatat
      241 cgacagatta atttatctat cgctggagaa aatcagaaaa gatttcgtgt ttatcaatct
      301 caatacggat tctttaactg aatttataaa ccgtgataat gagtggttat ccgcggtaaa
      361 ggggaaacag gtcgtattga ttgcggccag aaagtcagaa gccttagcaa attattggta
      421 ttacaacagc aatattaggg gcgtggtata cgctggactg agtcgtgata ttagaaaaga
      481 actggcctat gtgattaatg gcaggttcct gagaaaagat attaagaaag ataaaatcac
      541 tgaccgagaa atggaaatta tccgcatgac ggctcaggga atgctgccta aatcgattgc
      601 cagaattgaa aattgtagtg taaagacagt gtatacccat cggcgtaatg cagaggccaa
      661 gctgtactca aaaatatata agttggttca gtaaactcca ggcaagttag ttttaaaaaa
      721 tgactcactg ggacatcacg tcctcaattc aactcgggaa gaaatgcaat gaaaaaaaag
      781 gttctggcaa tagctctggt aacggtgttt accggcatgg gtgtggcgca ggctgctgac
      841 gtaacagctc aggctgtagc gacctggtcg gcaacagcca aaaaagacac caccagtaag
      901 ctggttgtga cgccactcgg tagcctggcg ttccagtatg ccgaaggcat taaaggtttt
      961 aactcacaga aaggtctatt tgacgtggct atcgagggtg actcaacggc taccgccttt
     1021 aaactgacct cacgtcttat caccaacacc ttaacccagt tggatacctc aggttctaca
     1081 ctgaatgtgg gcgtggatta taacggcgcg gcagtcgaaa aaactggcga taccgtgatg
     1141 atcgataccg ccaacggcgt actgggcggc aaccttagcc cgctggctaa cggttacaat
     1201 gccagcaatc gtaccaccgc acaggatggt ttcactttct ccatcatcag cggcaccacc
     1261 aatggtacca ccgcagtaac cgattacagc actctaccgg aaggcatctg gagcggcgac
     1321 gttagcgtac agttcgacgc gacccggacc agttaatctc tctgatgtac cagcaggggt
     1381 agcccccccc cctgctttat tcctggacgg aattgttatg aaaaagcacc ttctgcttct
     1441 cgctctgctg ttatccggaa tatctccggc ccaggcgctg gatgtcggcg atatatcatc
     1501 gtttatgaac agtgacagca gcacgctgag caaaacgatc aaaaacagta ccgacagtgg
     1561 tcgcctcatc aatatccgtc tcgaacggct ctcttcaccg cttgacgacg ggcaggttat
     1621 cgcaatggac aagccggatg agttgctact cactcccgcc agcttgctgc tacccgccca
     1681 agccagcgaa gtgatccgct tcttctataa gggaccagca gatgaaaaag agcgctacta
     1741 ccgcattgtc tggtttgatc aggccctcag tgatgcgcag cgcgataatg ccaaccgcag
     1801 cgctgtggcc actgcttccg cccgcatcgg caccattctg gtcgtcgcac cccgccaggc
     1861 aaactaccac tttcagtacg ccaacggctc cctgacaaat acaggaaatg cgacgctgcg
     1921 gatcctcgct tacggcccct gcctgaaagc cgccaatggt aaggagtgta aagagaatta
     1981 ctacctgatg ccgggcaagt cgcgtcgttt tacccgcgtg gacacggcgg ataacaaagg
     2041 acgggttgca ctttggcagg gtgataagtt cattcccgtg aaatagatag ctgtgcagat
     2101 ggataacgac aatgccttta cgacggttct ccccaggact gaaagcccag tttgccttcg
     2161 gcatggtctt tttgttcgtt cagcccgatg ccagcgctgc tgacataagt gcgcagcaaa
     2221 taggtggggt gattattccg caggccttca gtcaggcgct ccaggacggc atgagcgtcc
     2281 cgctttatat tcatctcgcc ggtagccagg gtcgccagga cgatcagcga atcggcagcg
     2341 cttttatctg gttggatcat ggacagctac gcatccggaa aatacagctg gaagagagtg
     2401 aagataacgc cagtgtcagc gaacaaactc gacagcagct gatggctctg gcgaacgccc
     2461 cgttcaatga ggcccttacc atccccctga ctgacaacgc gcagctggat ctcagcttgc
     2521 gccaactgct gctgcagctg gtggtcaagc gcgaagcgct gggcactgta ctacgctcac
     2581 gtagcgaaga catcgggcag tccagtgtta acaccctcag cagtaatctg agctataact
     2641 tcggcatcta taacaaccag ttgcgtaacg gcgggagcaa cacatccagc tatctgtcgc
     2701 tgaataacgt tactgcactg cgcgaacatc atgtggtgct cgacggctcg ctgtacggga
     2761 tcggtagcgg tcaacaggac agtgaattat ataaagcgat gtatgaacgc gattttgccg
     2821 gtcaccgatt tgccggtgga atgctcgaca cctggaactt gcagtcctta gggccgatga
     2881 ccgccatttc agcagggaag atttacggcc tttcctgggg aaaccaggcc agctccacca
     2941 tcttcgacag cagccagtca gccacgccag tgatcgcctt tttaccggcg gcgggcgaag
     3001 tacatctcac ccgtgatggg cggctactaa gcgttcagaa cttcactatg ggcaatcatg
     3061 aagtggatac ccggggtcta ccgtacggga tttacgatgt ggaagttgag gtgatcgtta
     3121 acggtcgcgt gatcagcaaa cgcccccagc gggtcaataa gctgtttagc cgggggcgcg
     3181 gcgtcggtgc accactggcg tggcaggtat ggggcggtag ctttcatatg gatcgctggt
     3241 cggaaaacgg gaaaaagacg cgaccagcta aagagagttg gctagcaggt gcctcgacct
     3301 ccggctcatt gagtacgctt agctgggcgg caacgggata tggatacgat aatcaggcgg
     3361 tgggtgaaac ccgtctgacg ctgccgcttg ggggagcgat caacgttaac ctgcaaaata
     3421 tgctggccag tgacagctca tggagcaaca tcgccagcat cagcgccact ctaccgggag
     3481 gctttagttc gctgtgggtt aaccaggaaa aaacccgcat tggcaatcaa ttgcgacgta
     3541 gcgatgccga caaccgtgca atcggcggca cactcaacct gaactcactg tggtcgaagc
     3601 tgggcacatt cagcatcagc tacaatgatg accgccgtta caacagccat tattacacgg
     3661 cagattacta tcaaaatgtc tacagcggtc cctttggttc gcttggcctg cgggccggta
     3721 ttcagcgcta taacaacggc gacagcaacg ccaatacagg gaaatatatc gctctcgatc
     3781 tctcgctacc actgggcaac tggtttagcg cagggatgac tcatcaaaac ggctacacca
     3841 tggcaaacct gtcagcacgc aagcagtttg atgaagggac cattcgcact gttggtgcca
     3901 atctgtcacg agccatctcc ggcgataccg gtgatgacaa aaccctcagc ggtggggcgt
     3961 atgcacagtt cgacgctcgt tacgccagcg gaacgctgaa cgtcaatagc gcggcggacg
     4021 gctacgtcaa taccaattta accgccaatg gcagcgtcgg ctggcagggt aaaaacattg
     4081 ctgccagcgg gcggactgat ggcaacgctg gggtgatatt caacaccggg ctggaggacg
     4141 acggtcagat cagcgccaaa atcaacgggc ggattttccc gcttaacggc aagcgtaact
     4201 atctcccgct ctctccctat ggaagatatg aggtggagtt acagaacagc aaaaactcac
     4261 tcgacagtta cgatatcgtc agcggccgca aaagtcatct gactctctat ccaggcaatg
     4321 tcgctgtcat tgagccagag gtgaagcaga tggttaccgt ctccggccgt atccgtgcgg
     4381 aagacggcac actgctggct aacgcacgga ttaacaacca tatcggccga acccgaaccg
     4441 atgaaaacgg cgagtttgtc atggatgtgg acaagaaata ccccactatc gattttcgct
     4501 acagtggcaa taaaacctgc gaagtggcac tggaactcaa ccaggcgcgc ggtgccgtct
     4561 gggtcggaga tgtggtctgc agcggcctct catcgtgggc ggcggtgacg cagacaggag
     4621 aagagaatga gagttaacct actgatagcg atgataatct ttgcgctaat ctggcctgca
     4681 accgcgctca gagctgcagt gagcaaaaca acctgggcgg atgcaccggc acgcgagttt
     4741 gtgtttgtcg aaaacaactc agacgacaac tttttcgtca ctcctggcgg ggcgctggat
     4801 ccgcgcctga ccggtgccaa ccgctggacc ggtttaaaat acaatggttc aggaaccatc
     4861 tatcagcaaa gcctcggcta cattgataac ggttacaaca ccggccttta taccaactgg
     4921 aagtttgata tgtggctgga aaattcacca gtttcatctc ctttaactgg cttgcgctgc
     4981 atcaactggt acgctgggtg taatatgacc accagtctta tcctgccgca aaccaccgac
     5041 gccagtggat tttatggcgc gacggtgacc agcggcggcg cgaagtggat gcacggcatg
     5101 ttgtcagacg cgttttacca gtatctgcaa caaatgcccg tcggcagcag ctttacaatg
     5161 accatcaatg cctgccagac ctctgtgaac tatgacgcca gcagcggcgc acgctgtaag
     5221 gatcaggcct ccggcaactg gtatgttcgc aacgtcaccc atacgaaagc agcaaatcta
     5281 cggttgataa atacccactc gctggcggaa gtatttatca acagcgacgg agtaccgact
     5341 ctgggcgaag ggaacgccga ctgccggacg caaaccatcg gcagccgttc aggattaagt
     5401 tgtaagatgg ttaactatac cctgcaaaca aacggactca gcaacacctc aatccatata
     5461 ttcccggcga tcgccaactc gtcgttagcc tcggccgtcg gggcgtacga tatgcagttc
     5521 agtctgaatg gcagttcatg gaaaccggtg agcaatactg cctattacta caccttcaac
     5581 gagatgaaga gcgcagactc gatctatgtt ttcttctcga gcaacttctt taagcagatg
     5641 gtgaacctcg ggatcagcga tatcaacacc aaagatctat tcaactttcg ctttcagaac
     5701 accacatcac aggagtctgg ctggtatgaa ttttctacct ccaacacgct gattatcaaa
     5761 ccccgtgatt tcagcatcag tattatctcc gatgaatata ctcagacacc gtcgcgggag
     5821 ggatatgttg gcagcggcga gtcggcactc gatttcggct atatcgtaac caccagcggt
     5881 aaaacagctg ccgacgaagt gctgatcaag gtgaccggac ccgcgcaggt gattggcggg
     5941 cgctcctatt gtgtcttcag ctccgatgac ggtaaggcga aagtaccgtt cccggcgacg
     6001 ctttccttta ttacccgcaa cggagctaca aaaacctacg atgccgggtg cgatgatagc
     6061 tggcgtgata tgaccgatgc gctgtggctg accacaccgt ggactgatat ctctggcgaa
     6121 gtggggcaga tggataagac cacagtcaaa ttttcgattc caatggataa cgccatttcg
     6181 ctgcgtacgg tagatgataa cggctggttt ggcgaagtca gcgcttcagg agaaattcat
     6241 gttcaggcga cgtggcgtaa cattaactaa ggccctgctg acagcggtct gtatgctggc
     6301 gacacctttg acacaggcga tttcggtcgg caatctgaca ttttcgctgc cgtccgagac
     6361 tgactttgtc agcaaacgtg tagtgaataa caacaaaagc gcacggatat accgtattgc
     6421 catcagtgct attgatagcc cgggcagcag tgaattgcgc acccgaccgg tggatggtga
     6481 actgcttttc gccccccgcc agctagcgtt gcaggctggt gagagcgagt attttaaatt
     6541 ttactatcat gggccacggg ataaccgcga gcgctactac cgggtctcat ttcgcgaggt
     6601 ccccactcgt aaccagacaa gacgtagccc aaccggcggc gaggtcagca cggagccggt
     6661 ggtggtgatg gataccattc tggtagtacg accacgtcag gttcagttta aatggtcctt
     6721 cgacaaggtg acaggaacgg taagtaacac tggcaacaca tggtttaagc tactgattaa
     6781 gccagaatgt gattcgaccg aagaggaagg cgatgcctgg tatctacgtc cgggagacgt
     6841 ggttcatcag cctgagttac gtcagccggg gaatcattat ctggtctata acgacaaatt
     6901 cattaagatt agcgattcct gtccggctaa gcccccttcg gcggactaag acttatccat
     6961 cggtcgaggg aagaattcca cctcagagcc ccaaaaatcg tttaaatgat gtggaagcga
     7021 tcgtcaatat cacctgtaac gatgggcttt ctcatatgca caccatagtg ttcaggagac
     7081 acggaaaggt atcaggcagc ctgctaacga gtggtttaat cacgaattag tacgtaaaat
     7141 cggtaacggc tggaaatcat tcaatactca cactatcgaa agttccccag ccaaccgcgg
     7201 tacgttctta catacgatgt accgctgttc tctttacgat ttttagctgt actggtgaat
     7261 tatgagcaat ctgaatccat gcatgacgtg tggtgcctgt tgtgcatttt tccgcgtctc
     7321 tttttactgg gccgaagccg acgatgctgg cggcactatt cccgccaggc tcactgaaca
     7381 aatatcccct tttcaccgat gcatgagcgg taccaatcag aaaaaccccc gatgtattgc
     7441 ccttgcagga accccgggca aaaatgcctg ctgcacgata tataaaaatc gatcgtccac
     7501 atgcagagaa tttgccatgt ctggtgagaa cggagtcgtc aatgaggctt gcaatcgtgc
     7561 aagggctaaa tacgggctga caccgttata aacatacaat aattaattgc actgccccgc
     7621 cagcgataat agcggggctt cgtttttcag gggtaacaaa acccgatact tcttctattt
     7681 gccagcaaca atgcctctct tctgtagcgt tcttcggatc tacatcatcc tgaagtagcg
     7741 cgatatactc actgactatc tgaatcagaa tataaaaagc aatgttttta acctataaaa
     7801 atggcgctgt atttgcgcca tttttatcat tcaatgcatt atctgtttga gcctaaaggg
     7861 atctcagggt ctggctcatg agtaattctg tttcgtaaat ctctgcgaat aatttcaata
     7921 gaccagaacc agactaaatg tccaaaaatt tcagaaacat tctcatacca cgggagatca
     7981 aacagaggtg gcgtcagtcc catgagaggg aatgaaatca tatgaacaaa aagttgggct
     8041 aaagcacctg ccagtaagcc ctgccagagt ttaatttttg gaaatacttc agcgaccaca
     8101 caataaccga cagcaaacac tatcgaaaag ataatgtgcg taacaccaac ccagttaaag
     8161 acatgcccgg caaaggtata aacagccgca ttgggatctg tcagccccaa ccagtctcga
     8221 agaaaaatat acggtggatt gagaaaatta cgcgagcaat caatttggcc tgcagccctg
     8281 attaatgatt ccgggccaca cgctgcatta aacatatcca ccgggctacg tggcggcaat
     8341 ggaacttcag ccccacttca cgaatgcgga aacaacgcca gcaatcagcc caatgaatgc
     8401 agcaagacca taacgtctgc ggttcggtgg agtttgttca aatatattca tatctaccct
     8461 gcttgtacca ttatgttata cacctcttca ggagtattca taaaacaagg taaatgtaaa
     8521 gaactgtatt gttttgtata acaagatggt ttcctaatcg ccaatgaata taagctccat
     8581 catttctccc tatttttata ttagaagtga cagagatttg cagggtgatg cagagctgaa
     8641 aatcacaggt ttccttattg gtttttgcat cgtacaacta aagcaataaa ccagctccca
     8701 tcatcatcaa catcccggcg aacatgaatt tattagccgg gctcatagct gcattcgcgt
     8761 tttaagaata atcctcctgc tgtcgccgac tatgcttaac atttaaaaaa gcatcagcac
     8821 tctcgcaacg cactcttatt ttccccttta gaataccgga ggcctggtat gagcaaccaa
     8881 ggcgaatacc ccgaagataa tcgggttggg aagcacgagc cgcacgattt aagtttgacc
     8941 cgtcgcgatc tgattaaagt gagcgccgca acagcggcga ccgccgtggt ttatcctcat
     9001 tctacgctgg cggcaagcgt tccggcagct acacccgcgc cagagataat gcccctgaca
     9061 ctgaaggtga acggcaaaac cgagcagctt gaggtggata cccgaaccac gctactggac
     9121 gctttgcgtg aaaatctgca tttgatcggt accaagaaag gttgcgatca cggacagtgc
     9181 ggagcctgta ccgtgctggt caatggtcgc aggcttaatg cctgcctgac gcttgcagtc
     9241 atgcatcagg gggccgagat caccaccatt gaaggcctgg gttcgccaga taatcttcac
     9301 cccatgcagg cggcctttat caagcatgat ggcttccagt gcggctactg cacctccggg
     9361 caaatttgct catcagtagc ggcgctaaaa gagattcagg acggcattcc cagtcacgtc
     9421 acggtcgatt tggtttccgc tccagaaaca actgccgatg agatccgtga acgtatgagc
     9481 ggcaacatct gtcgctgtgg tgcatacgct aacatccttg ccgccattga agatgctgcg
     9541 ggggagataa aatcatgaag gcgtttacct atgaacgagt gaacacccca gccgaggcgg
     9601 cacttagcgc tcagcgcgta cccggcgcaa aatttatcgc gggcgggacc aatctgctgg
     9661 acctgatgaa gctggaaatt gaaacgccca cccaccttat cgatgtgaac ggcctcgggc
     9721 tcgataagat tgaagtgacc gacgcgggtg ggctgcgcat cggcgcactg gtacggaaca
     9781 ccgacctggc ggctcacgag cgcgtgcgtc gtgattacgc ggtactctcc cgcgccctgc
     9841 tcgctggcgc atctggtcag ttacgtaatc aggcaaccac cgcaggtaat ctgctccagc
     9901 gcacgcgctg cccctatttt tacgacacca atcagccctg caataagcgc ctgcccggga
     9961 gcggctgcgc ggcgcttgaa ggctttagcc gtcagcacgc ggtggtaggc gtaagtgaag
    10021 cctgcatagc cacccatccg agcgatatgg cggtcgcaat gcggttgctg gatgcggtgg
    10081 tggaaaccat cacgccggag ggaaagactc gcagtatcac actggctgat ttttatcacc
    10141 caccggggaa aacgccgcac attgaaaccg ccctgcttcc cggtgagctt atcgttgcgg
    10201 tgacgttacc tccaccgctc ggcggaaaac atatctaccg taaggtgcgc gatcgcgcct
    10261 cctacgcctt tgccctggta tcggtcgcgg cgattattca gcctgacggc agcgggcgcg
    10321 tcgcgctggg cggagtagca cataagccct ggcgcattga ggctgcggat gctcagctat
    10381 cccagggggc gcaggccgta tatgacgcgc tgttcgccag cgcccatccc accgctgaaa
    10441 atacctttaa actcctgttg gcgaagcgaa cgcttgcctc cgtactggct gaagcgaggg
    10501 cacaggcatg aaatttgata aacccgcagg ggaaaacccg atcgatcagc tgaaggttgt
    10561 cggtcgtccc catgaccgca tcgacggacc gctgaaaact accggcacgg cacgctacgc
    10621 ctacgaatgg catgaagaag cccccaacgc cgcctatggc tatatcgtcg gttccgccat
    10681 tgccaaagga cgcctcaccg cccttgatac ggacgccgcg caaaaagcgc cgggcgtact
    10741 ggctgtcatt accgccagta acgccggggc actcggcaaa ggcgacaaaa acaccgccag
    10801 actgttaggc ggccccacta ttgagcacta tcatcaggcc attgcgctgg tagtggccga
    10861 gaccttcgaa caggcgcgag cggcggcctc gctggtgcag gcacactatc gccgtaataa
    10921 aggagcttac tccctggcgg acgaaaaaca ggccgtcaat cagccgccgg aagacacgcc
    10981 cgacaaaaac gtcggtgact ttgacggggc tttcacctcc gctgcggtga agattgatgc
    11041 tacctacacg accccggacc agagccatat ggcgatggag ccgcatgcct cgatggccgt
    11101 ctgggatgga aataagctta ccctctggac ctcaaatcag atgattgact ggtgccgcac
    11161 cgatctggca aaaacgctga aagttcccgt ggagaatgtg cgtattatct ccccgtatat
    11221 cggcggaggg tttggcggca agctgttcct gagaagcgat gcgctgctgg cggccctcgc
    11281 cgcccgagcg gtgaaacgtc cggttaaagt gatgctcccc cgcccctcta ttcccaataa
    11341 caccacgcac cgccccgcca cccttcagca cttgcgtatc ggtgccgacc agagcgggaa
    11401 aatcaccgct atctcacatg aaagctggtc tggaaacctg cccggcggca cgccggaaac
    11461 ggcggtacag caaagcgaat tactctacgc cggggcgaat cgtcataccg gcctgcggct
    11521 cgccacgctt gatttgccgg aagggaacgc catgcgtgcg cccggcgaag cccccggtct
    11581 gatggcgctc gaaatcgcga tcgacgaact ggcggaaaaa gcgggcatcg atcccgtcga
    11641 gtttcgcatc ctgaatgaca ctcaggttga ccccgccgac ccgacgcgct gcttctctcg
    11701 ccgtcagctt atcgagtgct tgcgcaccgg agcggataaa tttggctgga agcagcgcaa
    11761 cgccacaccc ggacaggtgc gcgacgggga gtggctagtc ggccacggtg ttgcggcggg
    11821 ctttcgcaat aatctgctgg aaaaatcggg tgctcgggtt cacctcgaac aaaacggcac
    11881 cgttaccgta gaaacggaca tgaccgacat tggcaccggc agctacacca ttctggccca
    11941 gacggcagcg gaaatgcttg gcgtaccgct ggagcaggtt gcggttcacc tcggcgattc
    12001 cagtttcccg gtttctgcgg gttctggagg acaatggggc gcgaatacct ccacctccgg
    12061 cgtttacgcc gcctgtatga agcttcgcga aatgattgcc tcggcagtcg ggtttgatcc
    12121 tgagcagtcg cagtttgccg acggcaagat taccaacggt acccgaagcg ccacgctaca
    12181 tgaagccacc gcaggcggca gactgacagc ggaagagagc attgaattcg gaacactgag
    12241 caaagagtac cagcagtcga cctttgccgg gcattttgtg gaggtcggcg tgcatagcgc
    12301 gacgggagaa gttcgggtcc ggcgtatgct cgctgtgtgt gctgcaggac gcatcctgaa
    12361 tccgaaaact gcgcgcagcc aggtcattgg cgcaatgact atgggcatgg gcgcggcact
    12421 gatggaggag ctggcggtgg atgaccgttt gggctacttc gttaatcacg atatggcggg
    12481 gtatgaggtg ccggtccatg cggatatccc aaaacaggag gtgattttcc tggatgatac
    12541 cgaccccata tcctccccga tgaaggccaa aggtgtcggt gagctgggcc tgtgcggcgt
    12601 gagcgcggct atcgccaacg cggtgtataa cgccaccggt attcgggtac gggattatcc
    12661 catcactctg gataagctgc tcgataagct gccggatgtg gtttaaggag aaacaatgtc
    12721 atacccgctt tttgataaag acgaacactg gcataagcca gagcaggcgt ttctcaccga
    12781 tgaccacctg accattctgc gcttcgccgt agaggcgcta atgtccggta aaggagcggt
    12841 gctggtgacg ctggtggaga tacgcggcgg cgcagcgcgc ccgctcgggg cgcagatggt
    12901 ggttcgcgaa gatggtcgtt actgcggttt tgtctctggc ggctgcgtgg aggccgctgc
    12961 cgcttttgaa gcgctggaga tgatgggctc aggccgcgat cgcgaaattc gctatggcga
    13021 aggttcgccg tggtttgaca tcgttctgcc ctgcggcggt gggatcacgc tgacgctcca
    13081 taaactacgc tcggcacagc ctctgctcgc cgtgctgaac cgtctggaac agagaaaacc
    13141 ggcggggctg cgctacgatc cgcaagcaca atcgctggtg tgcctgccca cgcaaacccg
    13201 aacgggctgg aatctcaatg gctttgaggt ggggttcagg ccatgcgtca ggctgatgat
    13261 ttacggacgt tctcttgaga cgcaggcaac tgcgagtctt gcagcagcca caggctatga
    13321 cagccatatc ttcgatcttt ttccggcctc agccagcgct cagatcgata ccgatacggc
    13381 ggtcattttg ctgtgccatg atctcaaccg ggagctgcca gtgttgcagg ccgcgcgaga
    13441 agcaaaaccc ttttatctcg gcgcattggg cagctatcga acccacacgt tacgtctgca
    13501 aaagctccac gagctgggat ggtccaggga ggaaacaacg caaatccggg cacccgtcgg
    13561 gatattcccc aaagcccggg atgcgcatac tctggcactc tccgtgctgg cagaagtcgc
    13621 ctctgtacgt ctccatcagg aggaggattc atgcctgccc ccgtcgtcct gatccttgcg
    13681 gccgggcgtg gagagcgctt tctcgcctcc gggggaaata cccataagtg tatcggctgg
    13741 cgtcagtccc cggaggttgc gccttatcgc tggccatttg aagaaaacgg gagaactttc
    13801 gaccttgcga ttgaaccgca gattacgact aatgatctgc gtctgatgtt gaggctggct
    13861 cttgccggcg gaggaataac aattgccact caggaaactt tcaggccata tattgaaagc
    13921 ggtaagcttg tatcgctgct tgatgacttt cttccacaat ttccgggctt ctatctgtat
    13981 ttcccacagc gtcgcaatat tgcaccaaag ctccgcgccc tgattgacta cgtcaaagaa
    14041 tggcggcagc aattggttta aatagctgca cctgcactgc ctgatgtcag aacagtattt
    14101 tgatgaattg ccaaggttac aatggcacaa atgcggcaca ggaggaaacg tggtgtattt
    14161 aaatatgggg taactctttg attttaatgg tgccgataat aggagtcgaa cctacgacct
    14221 tcgcattacg aatgcgctgc tctaccaact gagctatatc ggccctgaaa ggacatgttc
    14281 acgaacgtga atcacggtgg acaaggttaa aactaaccgg gcgatgcgtc aatggccttg
    14341 tgaatcaaat ggctactttt gcatcacccg gttttattta cgcacgaatg gtgtaatcac
    14401 caatgccgat ccacttgtaa gtggtcagtg cttccagccc cattgggcca cgcgcgtgga
    14461 gtttttgtgt gcttaccgcc acttccgcac ccagaccaaa ctggccgccg tcggtaaaac
    14521 gcgtagaggc gttaacgtaa acagcggacg aatccacttc gttaacaaaa cgctgggcgt
    14581 tgcgcatatc gcgggtcagg atcgcatcgg agtgttgtgt gccgtgttca cgaatatggg
    14641 cgatggcatc gtcaagatcg ctgacgattt tgacgttcaa atctaatgac agaaactcat
    14701 cgtcatactc ttcggcttta acagcaacca ccttcgcagg gcctgcctgc aactgcgcca
    14761 gtgcagctgc atctgcgtgt aatgtcacgc cgctttccgc catttgtttg cttaatgcgg
    14821 gcaggaagct atcggcgatg tttttattca ccagcaacgt ttcaaccgta ttacatgtgc
    14881 tcggacgctg agttttcgcg ttgacgatca cttttaatgc ttcagcgatc tctacacttt
    14941 catcaacgta aatatggcat acgcctatac cacctgtgat caccgggatt gtcgactgtt
    15001 cacggcacag tttatgcaaa ccagcgccac cacgcgggat cagcatgtcg atgtatttat
    15061 ccatacgcag catttcactg accagcgcac ggtcaggatt atcaatcgcc tgcacggcac
    15121 ccgccggtaa gccgcaggat ttcagggcgt cctgaatcac cgccaccgtt gcagcgttag
    15181 tgcgacacgt ttctttgcca ccgcgcagga tcaccgcatt accggttttc aggcacagcg
    15241 aagcgacatc aaccgtcacg ttcgggcgcg cttcataaat cacgccaata acccccagcg
    15301 gtacgcgacg acgctcaaga cgcaggccgc tgtccagtac gctgccatcg attacctgcc
    15361 ccaccggatc ggcgaggtta catacctggc gcacatcatc ggcaatgcct ttcagccgtg
    15421 cgggcgtcag tgccagacgg tcaagcatcg cttcgccaag gccattggca cgcgcgtcag
    15481 caacatcctg ggcgttagcg ttgaggatga tttcgctttg tgcttccagt tcatcggcga
    15541 ttttttccag cacgcgattt ttttcgcggc tggagagttg cgctaattta tacgaggctt
    15601 gcttcgcggc aatgcccatt tgttccagca tcagcctgct ccttaacggg taatcatgtc
    15661 atcacggtga acggcaaccg ggccgtattc atatcccagt attgcatcaa tttcttgcga
    15721 gtggtgcccg gcaatacggc gtaatgcatc gctgttgtaa cgactgacgc cgtgggcgat
    15781 atcgcgacct tcgaggttgc aaatgcggat gacttcacca cgcgagaaat tgccagtcac
    15841 gcttttaatg cctttcggca acagggagct gccgcgttca agaatggcgg cagttgcccc
    15901 ttcatctacc gtgatttcac ccgccggcgg cgcaccgaaa atccagcgtt tacggttttc
    15961 aagcggagtc gcctgggcat ggaacagcgt accgacggaa atgccttcca tcacatcacc
    16021 aataacgccc ggcttgctgc ccgcggcaat aatggtgtcg atacccgcac ggcaagccac
    16081 gtcagcggcc tgcaatttgg tactcatgcc gccagttccg aggcctgaaa cgctgtcacc
    16141 ggcaatcgcg cgcagtgcgt catcaatgcc gtaaacatct ttaatcagtt ctgcctgcgg
    16201 attgctgcgc ggatcagcgg tatacaaacc tttttgatcg gtcagcagca acagtttatc
    16261 ggcacccgcc agaatcgccg ccagcgcaga aaggttatcg ttatcgccga ccttaatctc
    16321 tgccgtagcg acagcatcgt tctcattgat taccggaacg atattgttat cgagcaacgc
    16381 acgcagggtg tcgcgggcgt tcaggaagcg ttcacggtct tccatatcag cacgggtcag
    16441 cagcatctgc ccgacgtgaa tgccataaat cgaaaacagc tgttcccaca gttgaatcag
    16501 tcgactctgc cctaccgccg ccagcagttg tttcgaggcg atagtcgctg gcagttccgg
    16561 gtaccccagg tgctcacgtc cggcggcgat cgcgcccgac gtcacaataa caatccgatg
    16621 cccggcggca tgtaactgcg cgcactggcg aacaagttca acgatatggg cacggttcag
    16681 acggcgcgat ccgcctgtta gcacactggt gccgagtttt accaccagcg tctggctgtc
    16741 actcatgatt ctctgccatt caattttagg aaaaatgata tcaaacgaac gttttagcag
    16801 gactgtcgtc ggttgccaac catctgcaag caaagcatgg cgttttgttg cgcgggatca
    16861 gcaagcctag cggcagttgt ttacgctttt attacagatt taataaatta cc
//
LOCUS       DAZFXT010000100        16868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.100, whole genome shotgun sequence.
ACCESSION   DAZFXT010000100 DAZFXT010000000
VERSION     DAZFXT010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.100"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..499
                     /gene="ydeR"
                     /locus_tag="ACHKW0_003536"
     CDS             <1..499
                     /gene="ydeR"
                     /locus_tag="ACHKW0_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476307.1"
                     /translation="RLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTKEA
                     NVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEGTA
                     ENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALINV
                     IYTWQ"
     gene            558..1472
                     /gene="ydeQ"
                     /locus_tag="ACHKW0_003537"
     CDS             558..1472
                     /gene="ydeQ"
                     /locus_tag="ACHKW0_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476308.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNNVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVAAGTVQSVIGVTFIYE"
     gene            1806..4085
                     /gene="ydeP"
                     /locus_tag="ACHKW0_003538"
     CDS             1806..4085
                     /gene="ydeP"
                     /locus_tag="ACHKW0_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HFO7476309.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4333..4530
                     /gene="safA"
                     /locus_tag="ACHKW0_003539"
     CDS             4333..4530
                     /gene="safA"
                     /locus_tag="ACHKW0_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HFO7476310.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4605..5366
                     /gene="ydeO"
                     /locus_tag="ACHKW0_003540"
     CDS             4605..5366
                     /gene="ydeO"
                     /locus_tag="ACHKW0_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HFO7476311.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTMPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTMNALAI"
     gene            5770..7452
                     /gene="ydeN"
                     /locus_tag="ACHKW0_003541"
     CDS             5770..7452
                     /gene="ydeN"
                     /locus_tag="ACHKW0_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HFO7476312.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            7504..8661
                     /gene="ydeM"
                     /locus_tag="ACHKW0_003542"
     CDS             7504..8661
                     /gene="ydeM"
                     /locus_tag="ACHKW0_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0018209 - peptidyl-serine modification
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HFO7476313.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(8627..8749)
                     /locus_tag="ACHKW0_003543"
     CDS             complement(8627..8749)
                     /locus_tag="ACHKW0_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476314.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            8952..10637
                     /gene="yddA"
                     /locus_tag="ACHKW0_003544"
     CDS             8952..10637
                     /gene="yddA"
                     /locus_tag="ACHKW0_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HFO7476315.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISAVL"
     gene            10675..13047
                     /gene="yddB"
                     /locus_tag="ACHKW0_003545"
     CDS             10675..13047
                     /gene="yddB"
                     /locus_tag="ACHKW0_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HFO7476316.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHVFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSSVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADRISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFANQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            13104..15887
                     /gene="pqqL"
                     /locus_tag="ACHKW0_003546"
     CDS             13104..15887
                     /gene="pqqL"
                     /locus_tag="ACHKW0_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HFO7476317.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMFETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRTT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     YPVNTYTGVLLPK"
     gene            16249..>16868
                     /locus_tag="ACHKW0_003547"
     CDS             16249..>16868
                     /locus_tag="ACHKW0_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7476318.1"
                     /translation="MDKKQVTDLRSELLDSRFGAKSISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT         4913 a         3686 c         3723 g         4546 t
ORIGIN      
        1 aagacttcac aagaggttcc tgttagctac gttttgcgcg ttattcacag caactctcca
       61 ggccgccgat gtcactatca ctgttaatgg tcgggtagtc gctaaaccct gcactattca
      121 aaccaaagaa gctaacgtta atctcgggga tctttatacg cgcaatctgc aacaacctgg
      181 ttctgcatct ggctggcaca atattacttt gtcattaacc gattgtccgg ttgaaacaag
      241 tgcagtgacg gcaatcgtga caggttcaac tgacaatacg ggttattaca aaaatgaagg
      301 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
      361 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      421 aagagctatc acggtgaatg gaaacgcaag ccagggaacg atcgaggcgc taatcaatgt
      481 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      541 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      601 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      661 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      721 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      781 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      841 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      901 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      961 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgctcgt attcatatgt
     1021 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
     1081 ataacaatgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
     1141 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
     1201 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
     1261 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt ctctctgatg cgtaatggaa
     1321 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1381 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt tgcagccgga accgtccagt
     1441 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgatcttag tctatgcgct
     1501 ccattcacga aaattgcgcg gataattatt ttgtgaaggc tattatccta cacctgtaag
     1561 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1621 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1681 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1741 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1801 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1861 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccaggatgtt attgccatgt
     1921 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1981 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     2041 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     2101 gagatcacga gcttgaggct gcgggacgtc tcactcagcc tttgaaatat gatgccgtca
     2161 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     2221 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     2281 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2341 ccaacatgtg ccatgaaccg accagtgtgg gtttggcagc gagtatcggc gtgggtaaag
     2401 ggactgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2461 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2521 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2581 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2641 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2701 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2761 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2821 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2881 aacgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2941 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     3001 tctgcccact acgtggccac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     3061 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcaca ccacctcaag
     3121 cacctggaca tgctgcaatt gccagcatgc aagcaatatg tacagggcag gctcgagcat
     3181 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     3241 cgttaacgca attagatctg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3301 tgaccgcacg gcatagctat attctgccgg ttctgggacg tagcgagatt gacatgcaaa
     3361 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3421 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3481 cgcaggcagc actaccccag agcgtggtag cctgggagta tctggtggaa gattatgatc
     3541 gcattcgcaa tgacattgaa gctgtgctgc cagagttcgc cgactataac caacgcatcc
     3601 gtcatcccgg tggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3661 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3721 acagtaagct ggtcatggcg acagtacgca gccacgatca gtacaacacg acgatttatg
     3781 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3841 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgctcacgc
     3901 cagacggtaa acgtagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3961 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaaca ctcgataacc
     4021 acgatccatt aagtggcatt cctggctata aaagtattcc ggttgaatta gaaccatcaa
     4081 attaatgcct cttctcattt cttctgctgt catccgcaca gcagaagaat ccctcattga
     4141 ctattatcgc gcaatttgct cacatggatt aaattaaact acatactata agatataaac
     4201 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     4261 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4321 aacatatttg aaatgcatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4381 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4441 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4501 catttcatta tcaatagttt gcaagactga taataaacag aaaattataa atctgaaatg
     4561 ttaacaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4621 ctgtcatatt tattcatcat gctttcaacg ctaacatttt agataaagat tacgccttct
     4681 ctgacggcga gatcctgatg gtagataacg ctgttcgtac acattttgaa ccttatgagc
     4741 ggcattttaa agaaatcgga tttaatgaaa ataccattaa aaaatatcta caatgcacta
     4801 acatccagac agtgacgatg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4861 ctggattgct taatgaaatg attgcttatc tcaactcgga agaacgcaat catcataatt
     4921 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4981 cactattaac taacggtgtg ctatccgttt ccgggaaagt gagaaatatt gtcaacatga
     5041 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     5101 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     5161 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     5221 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     5281 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5341 acacgatgaa tgctttagct atttgattat ttgctaacga gtagtcaacc acgcacccta
     5401 cgtaaaaatt aaaaatgggg cagtcattcc ctgccccgcg ttgtttttag gtgatatatt
     5461 tattgaaata aataagtgac attcatcaca tatttatgca tttgcataac ctgttgcatg
     5521 attatttatg atctcaattc ggcattttgt cagtaaaatg caataattta ttaaatatca
     5581 atagattagt tgtttatcgg caagaaatta cttagtagaa cagaaagtaa tgtcaacgct
     5641 ttatggactg ttatttccct ttttttagct aaatctgcta tctctttatg tgactaactt
     5701 cacttacacc cacttatttc tcttcgtaaa attacttcgg aattaagtac aataagaaga
     5761 ggaacattta tgaagtctgc attaaagaaa agtgtcgtaa gtacctcgat atctttgata
     5821 ctggcatctg gtatggctgc atttgctgct catgcggcag atgatgtaaa gctgaaagca
     5881 accaaaacaa acgttgcttt ctcagacttt acgccgactg aatacagtac caaaggaaag
     5941 ccaaatatta tcgtactaac catggatgat cttggttatg gacaacttcc ttttgataag
     6001 ggatcttttg acccaaaaac aatggaaaat cgtgaagttg tcgataccta caaaataggg
     6061 atagataaag ccattgaagc tgcacaaaaa tcaacgccga cgctcctttc attaatggat
     6121 gaaggcgtac ggtttactaa cggctatgtg gcacacggtg tttccggccc ctcccgtgcc
     6181 gcaataatga ccggtcgagc tcccgcccgc tttggtgtct attccaatac cgatgctcag
     6241 gatggtattc cgctaacaga aactttcttg cctgaattat tccagaatca tggttattac
     6301 actgcagcag taggtaaatg gcacttgtca aaaatcagta atgtgccggt accggaagat
     6361 aaacaaacgc gtgactatca tgacaacttc accacatttt ctgcggaaga atggcaacct
     6421 caaaaccgtg gctttgatta ctttatggga ttccacgctg caggaacggc atattacaac
     6481 tccccttcac tgttcaaaaa tcgtgaacgt gtccccgcaa aaggttatat cagcgatcag
     6541 ttaaccgatg aggcaattgg cgttgttgat cgtgccaaaa cacttgacca gccttttatg
     6601 ctttacctgg cttataatgc tccgcacctg ccaaatgata atcctgcacc ggatcaatat
     6661 cagaagcaat ttaataccgg tagtcaaaca gcagataact actacgcttc cgtttattct
     6721 gttgatcagg gtgtaaaacg cattctcgaa caactgaaga aaaacggaca gtatgacaat
     6781 acaattattc tctttacctc cgataatggt gcggttatcg atggtcctct gccgctgaac
     6841 ggggcgcaaa aaggctataa gagtcagacc tatcctggcg gtactcacac cccaatgttt
     6901 atgtggtgga aaggaaaact tcaacccggt aattatgaca agctgatttc cgcaatggat
     6961 ttctacccga cagctcttga tgcagccgat atcagcattc caaaagacct taagctggat
     7021 ggcgtttcct tgctgccctg gttgcaagat aagaaacaag gcgagccaca taaaaatctg
     7081 acctggataa cctcttattc tcactggttt gacgaggaaa atattccatt ctgggataat
     7141 taccacaaat ttgttcgcca tcagtcagac gattacccgc ataaccccaa cactgaggac
     7201 ttaagccaat tctcttatac ggtgagaaat aacgattatt cgcttgtcta tacagtagaa
     7261 aacaatcagt taggtctcta caaactgacg gatctacagc aaaaagataa ccttgccgcc
     7321 gccaatccgc aggtcgttaa agagatgcaa ggcgtggtaa gagagtttat cgacagcagc
     7381 cagccaccgc ttagcgaggt aaatcaggag aagtttaaca atatcaagaa agcactaagc
     7441 gaagcgaaat aactaaacct tcatgcggcg gaattttccg ccgccttatt gagcgagata
     7501 gcgatgcacg ttacagccaa gccctccagt tttcaatgta atctcaaatg tgattactgt
     7561 ttttaccttg aaaaagagtc gcagtttact catgaaaaat ggatggatga cagtactctg
     7621 aaagagttca tcaaacaata tatcgcagcg tctggcaatc aggtctattt tacctggcaa
     7681 ggcggtgaac ccactctggc tggcctggat tttttccgta aagttattca ctatcaacaa
     7741 cgctatgcag gccaaaaacg tatttttaat gcattacaaa cgaatggcat tttattgaat
     7801 aatgaatggt gtgcctttct caaagaacat gaatttctgg ttggtatctc gatcgatggc
     7861 ccccaggagt tacatgaccg ttacagacgc agtaattcag gtaacggtac ttttgcaaaa
     7921 gtgatagcag ccatcgagcg tctgaaatca tatcaagttg agtttaatac gttaaccgtc
     7981 attaataacg ttaatgtcca ttaccctctt gaggtttatc attttttaaa atctatcggc
     8041 agtaaacata tgcaatttat cgaattgctc gaaaccggga cgccgaatat tgatttcagt
     8101 ggtcatagtg agaacacatt ccgtatcatt gatttttctg tgcctcccac ggcttatggc
     8161 aagtttatgt caaccatttt tatgcaatgg gttaaaaacg atgtgggtga aattttcatt
     8221 cgtcagtttg aaagctttgt cagccgtttt ttggggaatg ggcatacaag ttgtattttc
     8281 caggagtcct gcaaggataa tctggttgtt gaaagtaatg gagacattta cgaatgcgac
     8341 cattttgtct atccacagta caaaattgga aacattaata aatctgaact caaaacgatg
     8401 aacagtgtac aactgacagc gcaaaaaaaa cggatttcag cgaaatgtca gcaatgtgca
     8461 tataaaccta tctgcaatgg cggttgtcct aagcatcgta ttactaaagt aaacaatgag
     8521 actgtttctt atttttgcga aggttataaa atcctttttt caaccatggt accttatatg
     8581 aacgccatgg tggagttagc taagaacaga gtaccgcttt accacattat ggatgttgca
     8641 aaacaaatgg agaataatta ataacgctct cttttaactc cattcttagc ctccttctta
     8701 tgaccttaac aaggcacccg acataaattc ctgtcgggtg tttcgtcatc atcatcttcc
     8761 cttgcgaaaa tcctggctta cgtggtatgc gtcagggcat tagcaatacc cttgattatc
     8821 aatagcataa ccataaccac tgaaaatgaa atattccttt atatttcata ttgttaaatg
     8881 atacttaaat aaatttcatc agtaaatcat tgccatagat aatcattgga tggcacaatc
     8941 aattttcgct aatgataacc attcccatta cgttttgtat gttgatagca aagtatctct
     9001 gtctgctgaa gcccttttgg ctgcgtaaga ataataaaac ttcagtgttg ttaatcatta
     9061 ttatcctcgc catgatcctt ggcgtggtta aaattcaggt ctggttaaac gactggaata
     9121 atgatttttt caatgcgcta agccagaaag aaaccgataa gctttggcag cttgtccttt
     9181 ggttccctgc cctgctgggg atctttgtat taatatccgt aaacaaaaca tggttaatta
     9241 aactactgac tattcgctgg cgcgaatggt taacagatta ctaccttaat cggtggtttg
     9301 cagataaaaa ctactacttc acgcagatct acggcgagca taaaaataca gataaccctg
     9361 accaacgtat cgctgaagac attctcttac tgatcagcaa aacgctcagc ctttcatttg
     9421 gcttcatcca gtcacttagc atgctgatca cctttaccgt tattctttgg caaagcgcgg
     9481 gtacgctctc attcactgtt ggtggaacag aatggaatat ccaggggtat atggtctata
     9541 ccgttgtgct cattgtgatc ggtggaactt tatttaccca taaggttggt aaacgtattc
     9601 gtccgcttaa tgtggaaaaa caacgtagcg aagcgacatt tcgaacaaat cttgtgcagc
     9661 ataataagca ggcagaactg attgcgctaa gcaatgctga gtctctacaa cgccaggagt
     9721 tgagcgacaa ttttcatacc attaaagaga actggcatcg tttaatgaat cgtcagcggt
     9781 ggcttgatta ctggcagaat atttattcgc gctcgcttag cgttcttccc tactttctgt
     9841 tattgccgca gtttattagc gggcagatca atctgggcgg actgatgaaa tcgcgccagg
     9901 catttatgct ggtatcgaac aatttaagct ggtttattta taaatatgac gaacttgctg
     9961 agctggctgc ggttatcgat cgcttgtatg agttccatca actcactgaa cagcgcccta
    10021 cgaataagcc taaaaattgc caacatgcgg tacaagtggc taatgcgagt attcgtacgc
    10081 ctgataataa gatcatatta gagaacttga actttcatgt ttcgccaggc aaatggctat
    10141 tactgaaagg ctactctggt gcgggaaaaa ccacactgct taaaacatta tcccactgct
    10201 ggccgtggtt taaaggtgat atttcttctc ctgctgacag ttggtatgtg tcacaaacac
    10261 cgttaatcaa aagcggctta ctgaaagaga ttatttgtaa agcacttccc ctgcccgtag
    10321 acgataaatc gttgagcgaa gtactgcatc aggttggtct ggggaaattg gctgcgcgta
    10381 ttcatgacca cgatcgctgg ggagatattc tttccagcgg cgaaaaacaa cgtatcgccc
    10441 tggcacgatt aattttacga cgtccgaaat ggatatttct tgacgaaact acctctcatc
    10501 ttgaggaaca agaggctatc cgcttactgc gtttagtgcg tgaaaaacta cccacaagcg
    10561 gcgtcattat ggttacacat caacccggtg tctggaacct ggcagatgat atttgtgaca
    10621 ttagcgcggt tttataaaaa caataaaagc cctgctgtac ggagatataa ataaatgaag
    10681 cgagttctta ttcctggcgt cattttatgt ggcgctgatg tggcgcaggc cgtcgatgac
    10741 aaaaacatgt acatgcatgt ttttgaagag atgacggtct atgctcctgt ccctgtaccc
    10801 gtaaacggca acacgcatta caccagtgaa agcatcgagc gtttaccgac cgggaatggc
    10861 aatatcagcg atctgctgag aaccaaccct gcggtacgca tggattcaac gcaaagtacc
    10921 tcgttgaacc agggagatat tcgcccggag aaaatctcta ttcacggtgc gtcgccctat
    10981 cagaatgcct atttgattga cggtattagt gccactaata acttgaaccc agcgaatgag
    11041 tccgatgcca gtagtgcaac caatattagc gggatgtcac aggggtatta tcttgatgtc
    11101 agcttactgg acaatgtgac gctttatgac agttttgtgc cggttgaatt cggtcgcttc
    11161 aatggcgggg taattgatgc aaagatcaaa cgcttcaacg ctgatgatag cagcgtgaaa
    11221 ctgggttatc gcactacgcg ttcggactgg ttaacatcgc atatcgatga gaataacaag
    11281 agcgcattta atcaaggctc ttcaggaagt acttattact ccccagattt taaaaagaac
    11341 ttttatacct tgtcgtttaa tcaggaactc gctgataact ttggcgttac cgccggttta
    11401 tcgcgccgcc agtctgatat cacccgcgcg gattatgttt cgaatgacgg cattgtcgcc
    11461 ggtcgggcac agtataaaaa cgttatcgat accgcattga gcaaatttac ctggtttgcc
    11521 agcgaccgct ttacccacga tttaacctta aaatataccg gctccagccg tgattataat
    11581 accagcacct tcccgcagtc tgatcgcgaa atgggtaata aatcctatgg tctggcatgg
    11641 gatatggata cgcagctcgc atgggccaaa ctacgtacca ccgttggttg ggatcatatt
    11701 agtgattata cccgtcacga tcatgacatc tggtacaccg aactttcatg tacatatggt
    11761 gatattacag ggcgttgcac ccgtggcgga ttaggacaca tttcccaggc tgtagataat
    11821 tacaccttca aaacacgcct ggactggcaa aaattcgccg tgggtaatgt ttcgcatcaa
    11881 ccctacttcg gcgcggaata catctattcc gatgcgtgga ctgaacgcca taaccagtct
    11941 gaatcctatg tgattaatgc tgccggaaag aaaactaacc ataccattta ccataaaggt
    12001 aaaggcagcc tgggaattga caactacacg ctgtatatgg cggatcgcat tagctggcgt
    12061 aatgtgtcat taatgcccgg cgtgcggtat gactatgaca actatctgtc aaaccacaat
    12121 atctccccgc gctttatgac ggaatgggat atttttgcta atcaaacctc aatgattaca
    12181 gcaggttata accgttacta tggcgggaat attcttgata tgggattacg tgatatccgc
    12241 aatagctgga cggaatcggt atcaggtaat aaaactctga cgcgttatca ggatttgaaa
    12301 acgccttata acgatgaact ggcaatggga ttgcagcaga aaatcggtaa gaacgttatt
    12361 gcgcgtgcaa actatgttta ccgtgaagcg catgatcaaa taagcaaaag cagtcgtacc
    12421 gacagcgcga ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat
    12481 tcgttcagcc tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac
    12541 ccacaaattg tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat
    12601 gaggagagca ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat
    12661 agcgttccag tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg
    12721 catcaaccga gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct
    12781 cgcattatcc tgggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag
    12841 tatgttgacg aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca
    12901 caatttctga aacaacaaaa cctgacgatc agtgccgata ttctcaatgt attggatagc
    12961 aaaaccgctg ttgatacaac gaataccggt gtggcgacct acgccagtgg ccgtactttc
    13021 tggcttgatg tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc
    13081 ccggttttcg catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa
    13141 ctctgttgct ccccgggcgg ctgattgccg ccgccttacc gcaggatgaa aagttaatta
    13201 ccgggcaact gggcaatggc ttgcgatata tgatttatcc gcatgctcat ccaaaggatc
    13261 aggtaaattt atggctgcaa attcataccg gttcattgca ggaagaagac aatgagcgcg
    13321 gcgtagctca ttttgtagaa catatgatgt ttaacggcac aaaaacatgg ccgggtaata
    13381 aagtcatcga aacatttgag tcaatgggcc tgcgttttgg tcgcgatgtt aatgcctata
    13441 ccagctatga cgaaacggtg tatcaggtga gtttgccgac tacgcagaaa caaaatctgc
    13501 aacaagtgat ggcaatcttc agtgaatgga gtaatgccgc aacctttgaa aaactcgaag
    13561 tagacgctga acgtggcgta attactgagg aatggcgtgc ccatcaggat gcgaaatggc
    13621 gcacctctca ggcgcgccgc cctttcctgc tggctaatac ccgtaattta gaccgtgaac
    13681 ctatcggcct gatggatact gtcgccacgg tcacaccggc acaattgcgc caattttatc
    13741 aacgctggta tcaaccaaat aatatgacct ttatcgtggt cggcgatatc gacagtaaag
    13801 aagcgctggc gctgataaag gataatttaa gtaagcttcc ggctaacaaa gcagctgaaa
    13861 atcgcgtctg gccgacaaaa gccgaaaacc acctgcgctt taatatcatt aatgataaag
    13921 aaaaccgggt gaacggcatc gcactctatt atcgcctgcc aatggtacaa gtgaacgatg
    13981 agcaaagctt tatcgaacaa gctgaatgga gcatgttagt tcagctgttc aatcaacgtc
    14041 tgcaggaacg catacagtcg ggcgagttga agactatttc tggcggcact gcgcgcagcg
    14101 ttaaaattgc acccgattat cagtcgctgt ttttccgtgt aaatgcacga gacgataata
    14161 tgcaggatgc tgcgaatgca ttaatggcag agttggcaac cattgatcag catggttttt
    14221 ctgctgaaga actcgatgat gtcaaatcta cccgcctcac ctggctgaaa aatgcggttg
    14281 atcagcaagc tgaacgtgat ttacgtatgc tgaccagtcg cctggcatcc agctcattaa
    14341 ataatacgcc gttcttgtcg ccggaagaga catatcaact ttcgaaacgt ctgtggcagc
    14401 aaattaccgt gcaaagtctg gcggaaaaat ggcagcagtt aagaaagaac caggacgcat
    14461 tttgggagca aatggtaaac aatgagcttg ccgccaaaaa agcattgtct cctgcggcta
    14521 tcctggcgct ggaaaaagag tacgccaaca aaaagctggc ggcttacatc ttcccaggca
    14581 gaaatttatc gttaacagta gacgctgacc cacaggcgga aattagcagc aaagaaacgc
    14641 tggctgagaa tctgacatca ttaacacttt ccaatggtgc cagggttatt ctggcaaaat
    14701 ccgcgggtga agagcaaaag ctacaaatta ctgccgtatc gaataaaggc gatttaagtt
    14761 tccctgcgca gcaaaaatca cttatcgcgc tggcaaataa agcagtaagc ggaagcggcg
    14821 ttggtgagct ctcctcttcc agcctgaaac gctggagtgc ggaaaattcg gtaaccatga
    14881 gcagtaaagt cagtggcatg aatacgttgc tctccgttag cgcgcggact aataaccctg
    14941 aacctggttt tcagttgatt aaccagcgaa tcacccacag cacgattaac gacaatattt
    15001 gggcatcgct acaaaatgct caaattcagg cgttgaaaac gctcgaccag cgtccagcgg
    15061 agaaattcgc ccagcagatg tttgagacgc gctatgctga tgaccgcacg aaattactgc
    15121 aagaaaatca gattgtacag tttactgccg cagatgcgct ggctgccgat cgccagttgt
    15181 tttcatctcc agcggatatc acgtttgtca ttgtcggtaa tgtctcagaa gacaaactcg
    15241 tggcgttaat tacgcgttac ttaggatcaa tcaaacactc tgattcgcca ttagccgcag
    15301 gtaaaccatt aactcgcacg acggacaacg catcggttac tgtaaaagaa caaaatgaac
    15361 ctgtggcaca ggtttcacag tggaagcgtt atgattcccg gacacctgtt aatctggcga
    15421 cgcgtatggc gctcgatgct tttaacgtcg cactggcaaa agatctacgt gttaatattc
    15481 gtgaacaggc atctggagca tacagcgttt cttctcgcct ctcggttgat cctcaggcca
    15541 aagatatcag tcatttgctg gcttttactt gtcaaccaga acgacatgat gaactgttaa
    15601 cgttagcgaa tgaagtgatg gttaagcgtc tggctaaagg gatcagtgag caagaactga
    15661 atgaatacca gcaaaacgtt cagcgcagcc tcgatatcca acagcgtagc gttcaacaat
    15721 tagcgaacac tattgtaaat agtcttattc aatatgacga tcctgcagca tggactgagc
    15781 aggagcaatt gttgaaacaa atgacggtag agaatgttaa cactgccgtt aaacaatatc
    15841 tttcttatcc ggtaaatact tataccggag tattattgcc aaaataataa cagccccgtc
    15901 aacacatcgt tggcggggat tttagcaata ttcgctattt ttatgtaata attttataaa
    15961 tgcgttcaaa ataataatca agtactaata gtgatatttt aaggtctgat ttttacgtga
    16021 taattcagga gacacagaat gcgcataaaa ataacagcat aaaacacctt accaccaccc
    16081 aagaatttca tattgcattg tttttcaatg aaaaaatatt attcgcgtaa tatctcacga
    16141 taaataacat taggattttg ttatttaaac acgagtcctt tgcacttgct tactttatcg
    16201 ataaatccta cttttttaat gcgatccaat cattttaagg agtttaaaat ggataagaag
    16261 caagtaacgg atttaaggtc ggaactactc gattcacgtt ttggtgcgaa gtctatttcc
    16321 actatcgcag aatcaaaacg ttttccgctg cacgaaatgc gcgacgatgt cgcattccag
    16381 attatcaatg acgaattata tcttgatggc aacgctcgtc agaacctggc cactttctgc
    16441 cagacctggg acgacgaaaa tgtccacaaa ttgatggatt tatccattaa caaaaactgg
    16501 atcgacaaag aagaatatcc gcaatccgca gccatcgacc tgcgttgcgt aaatatggtt
    16561 gccgatctgt ggcatgcgcc tgcgccgaaa aatggtcagg ccgttggcac caacaccatt
    16621 ggttcttccg aggcctgtat gctcggcggg atggcgatga aatggcgttg gcgcaagcgt
    16681 atggaagctg caggcaaacc aacggataaa ccaaacctgg tgtgcggtcc ggtacaaatc
    16741 tgctggcata aattcgcccg ctactgggat gtggagctgc gtgagatccc tatgcgcccc
    16801 ggtcagttgt ttatggaccc gaaacgcatg attgaagcct gtgacgaaaa caccatcggc
    16861 gtggtgcc
//
LOCUS       DAZFXT010000101        16845 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.101, whole genome shotgun sequence.
ACCESSION   DAZFXT010000101 DAZFXT010000000
VERSION     DAZFXT010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16845)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16845)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16845
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.101"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            343..528
                     /locus_tag="ACHKW0_003548"
                     /pseudo
     CDS             343..528
                     /locus_tag="ACHKW0_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(548..730)
                     /locus_tag="ACHKW0_003549"
                     /pseudo
     CDS             complement(548..730)
                     /locus_tag="ACHKW0_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            complement(1004..2023)
                     /locus_tag="ACHKW0_003550"
     CDS             complement(1004..2023)
                     /locus_tag="ACHKW0_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HFO7476319.1"
                     /translation="MKYTPVGVDIAKHLIQVHFIDENTGEVVDKQLRGRDFLEYFSNR
                     NPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRRQLVKFRTMQTNSLHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLITVLENQYNRLNELDLLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYVRNHV
                     SIRPY"
     gene            complement(2171..2335)
                     /locus_tag="ACHKW0_003551"
                     /pseudo
     CDS             complement(2171..2335)
                     /locus_tag="ACHKW0_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6404 family protein"
     gene            2454..2801
                     /locus_tag="ACHKW0_003552"
     CDS             2454..2801
                     /locus_tag="ACHKW0_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HFO7476320.1"
                     /translation="MKLDISVKYLLKSLIPSLIILTVFYLGWKDNQENARMFYAFIGC
                     IINAITFPFSMRIIQKMVIKITGKELWQKDFFTNPVGGSLTAIFELFCFVISVPVVAI
                     YLIFIFCKALSGK"
     gene            complement(2819..4699)
                     /locus_tag="ACHKW0_003553"
     CDS             complement(2819..4699)
                     /locus_tag="ACHKW0_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0031640 - killing of cells of another
                     organism [Evidence IEA];
                     GO_process: GO:0050829 - defense response to Gram-negative
                     bacterium [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colicin Ia central receptor-binding
                     domain-containing protein"
                     /protein_id="HFO7476321.1"
                     /translation="MSDPVRITNPGAESLGYDSDGHEIMAVDIYVNPPRVDVFHGTPP
                     AWSSFGNKTIWGGNEWVDDSPTRSDIEKRDKEITAYKNTLSAQQKENENKRTEAGKRL
                     SAAIAAREKDENTLKTLRAGNADAADITRQEFRLLQAELREYGFRTEIAGYDALRLHT
                     ESRMLFADADSLRISPREARSLIEQAEKRQKDAQNADKKAADMLAEYERRKGILDTRL
                     SELEKNGGAALAVLDAQQARLLGQQTRNDRAISEARNKLSSVTESLNTARNALTRAEQ
                     QLTQQKNTPDGKTIVSPEKFPGRSSTNHSIVVSGDPRFAGTIKITTSAVIDNRANLNY
                     LLTHSGLDYKRNILNDRNPVVTEDVEGDKKIYNAEVAEWDKLRQRLLDARNKITSAES
                     AVNSARNNVSARTNEQKHANDALNALLKEKENIRSQLADINQKIAEEKRKRDEINMVK
                     DAIKLTSDFYRTIYDEFGKQASELAKELASVSQGKQIKSVDDALNAFDKFRNNLNKKY
                     SIQDRMAISKALEAINQVHMAENFKLFSKAFGFTGKVIDRYDVAVELQKAVKTDNWRP
                     FFVKLESLAAGRAASAVTAWTFSVMLGTPVGILGFAIIMAAVSALVNDKFIEQVNKLI
                     GI"
     gene            complement(4978..6324)
                     /gene="yagA"
                     /locus_tag="ACHKW0_003554"
     CDS             complement(4978..6324)
                     /gene="yagA"
                     /locus_tag="ACHKW0_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YagA"
                     /protein_id="HFO7476322.1"
                     /translation="MPMILVKKNPRGKVIRELSSEEETAVKTVCGLKKPATMALHNLA
                     NDILREMREYDAWLQCDCIPGDSPAMNFAALKNNTGTLYLSSFNHEHAPECPMYRQLS
                     GNEEETSSGASRHPVSTRINYRNFLPPDDSNSVIRLQARSAYHNGESSSVRKKRPRLA
                     RLLLSLIDDAGLNKLDSLANPRIRTNYRECLDAIRQVTRQQEYIRGRALSEIIHFRPG
                     MSERSQERLMETLENSERHWPARRKHMFFQIFMAQHICRDAVEIHWANGNIQVIRPVR
                     GISINGEAQGGIRPPYWVILAFCRSADGRIICSEGYAHALYQLTCPVPVDSKLERNTL
                     TALLNVASWLKRKPGTPELSLERPLFDTEVYVNGEKKYVLPDFIVTARAPDGKTARVV
                     IETMGYEDSDYCARKSRQHTGMKQIGVLHTDPPKWLDNDHPPFEKHMYGVFMHLRY"
     gene            6678..7280
                     /locus_tag="ACHKW0_003555"
     CDS             6678..7280
                     /locus_tag="ACHKW0_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FINO family protein"
                     /protein_id="HFO7476323.1"
                     /translation="MGKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAEHPETELTMDEWLCAVSCITSRRVYLQRTAVAGVPRYGL
                     DGHPKGQVSDSEAQSAGRRLATLEQKWLRMQAQQENISGQ"
     gene            7336..7812
                     /locus_tag="ACHKW0_003556"
     CDS             7336..7812
                     /locus_tag="ACHKW0_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476324.1"
                     /translation="MCDSEAEKILFILNKHERAYRCQYPVYIFLAIVLWILPILLIIY
                     LHFCIGIGFCIAWTAYYFLSLFEYFVKYFFPLDREDLLNLMDLAKDSPDAKQELLERL
                     LSGKRMTGRDEMDIKRILKTKEALDNIGNFIDKQQLTNNDTPKKSTVPNEDKKGIR"
     gene            7812..8078
                     /locus_tag="ACHKW0_003557"
     CDS             7812..8078
                     /locus_tag="ACHKW0_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476325.1"
                     /translation="MITGNGINTVTVNGKVKHITELDYITLCLEWTKLREENNRLYEI
                     NNQANRGWRGFILRLIGVNLPDKRTEFTRRLLLTRNISGSVMKK"
     gene            complement(8347..8550)
                     /locus_tag="ACHKW0_003558"
                     /pseudo
     CDS             complement(8347..8550)
                     /locus_tag="ACHKW0_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8656..8931)
                     /locus_tag="ACHKW0_003559"
     CDS             complement(8656..8931)
                     /locus_tag="ACHKW0_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7476326.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIYVLRLWHTRENR"
     gene            complement(8931..9215)
                     /locus_tag="ACHKW0_003560"
     CDS             complement(8931..9215)
                     /locus_tag="ACHKW0_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family ribbon-helix-helix protein"
                     /protein_id="HFO7476327.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAWADSLSTDNPLPVPR"
     gene            complement(10120..10992)
                     /gene="repA"
                     /locus_tag="ACHKW0_003561"
     CDS             complement(10120..10992)
                     /gene="repA"
                     /locus_tag="ACHKW0_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006276 - plasmid maintenance [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HFO7476328.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     SSLDISEEAVASARRSRVEWENKLRKKQGLDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASP"
     gene            11695..12336
                     /locus_tag="ACHKW0_003562"
     CDS             11695..12336
                     /locus_tag="ACHKW0_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HFO7476329.1"
                     /translation="MCLSELQAGLNEGQPLFPTHALCIRRSQQHRSPSAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPERHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            12477..13142
                     /locus_tag="ACHKW0_003563"
     CDS             12477..13142
                     /locus_tag="ACHKW0_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HFO7476330.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALSAC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTRDTFSTTGRMLRPVAFSLIHP
                     ASLESSEVEEIFRDEGFILAHGRECSLNGSGRNMLSRILHVGHSGLPKAEWGIDHSNH
                     LLH"
     gene            13409..14029
                     /locus_tag="ACHKW0_003564"
     CDS             13409..14029
                     /locus_tag="ACHKW0_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold-containig protein"
                     /protein_id="HFO7476331.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            14056..15750
                     /locus_tag="ACHKW0_003565"
     CDS             14056..15750
                     /locus_tag="ACHKW0_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HFO7476332.1"
                     /translation="MFNLPGWLFTVISLVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTAREHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQAPLIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNSEPQQV"
     gene            15831..16073
                     /locus_tag="ACHKW0_003566"
     CDS             15831..16073
                     /locus_tag="ACHKW0_003566"
                     /inference="COORDINATES: protein motif:HMM:NF022090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HFO7476333.1"
                     /translation="MSAEKFRVVIYTNTCEKKVSYIPATEDINIAIMPFQTADNGLII
                     VCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNRVQL"
     gene            16190..16525
                     /locus_tag="ACHKW0_003567"
     CDS             16190..16525
                     /locus_tag="ACHKW0_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476334.1"
                     /translation="MRTITTHEQLLINGRVHERIATHIVTGAHGYETLCTNGYNVRYN
                     KAQQLVESCEKIAEGKLPVTCPVCFAIWQDVHRFTHVDFDTQSGKSDFIDTCHSEIIT
                     GNVSITGKK"
     gene            16682..>16845
                     /locus_tag="ACHKW0_003568"
     CDS             16682..>16845
                     /locus_tag="ACHKW0_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HFO7476335.1"
                     /translation="MQKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLL"
BASE COUNT         4305 a         4221 c         3908 g         4411 t
ORIGIN      
        1 atgtatttta ggatgaccta tgttgaatag aacctaaaac aaaacaaata ttaatagggg
       61 tccgcttgga aaagggaaat tttcccacgc taagagaatt gagttgaacg agtggttaca
      121 tccaggccag ctaactcact gaacgtaaag gctgtacgaa atgttccggc cgtccgataa
      181 ccgtttggct tacttccgta cattcccttg cgtagatgta cagaattgta cgatttcgga
      241 caccaaaacc gctgttgtcc cgacattctg gcgattcctg aaagtccttt gactaaaaaa
      301 tagtcttaac gtggtttatt ttccgaattc caagcggacc cctatcaggc ctgccgtggg
      361 gattttgtgg tgcgtctcga cggcagcacc tgtctgcagt tatggaataa agaaggcagg
      421 gtggtacgcc gggagggcga tccgctggaa gtggcgcagt ggctgctggc ctgtcatgac
      481 gcaggaatag aagttcgcgt acagataaat gaaagtgccg ctccgtaaga acacgcctgt
      541 gcacggttta cacatcatcc catggtggca gccggttaac tttcctgcgc caccagtgga
      601 aagaggccat gagcagtccg aaccaaaaac cccccgtgat acttataata atggcctcac
      661 ctgccaccat tcctgacggc ccccagtaga taaaccacat tgcacatccc caggaaatgc
      721 cccataagct ctgtatgaac gcatccccgt ttgcaaggct tccgttgttc tgacatcgac
      781 gagtagttgc agctctgtat ccggccttat tgcagtcaga gctgcgggcc actatgagat
      841 tcgctgacct gagccttatt cccattacgg actttatgtc caggagctta ttcaggttta
      901 tcaggtcacg gtcttacctg tcgttgtcat cactcgggcc gccatagcaa cctttcagca
      961 ttcacccttt tgttaagagt tcattgttta acggtatcag cgattaatat ggtctgatac
     1021 tgacgtggtt cctgacatac ttacggtcat gggccgttat cgcccatact gttcgtgcca
     1081 gcttgtttgc catggcgacg attgccacac tggccggacg acgttttttc agttcggtta
     1141 tccacgggcc tggttcttta gctactaatg ccaccgctct tgctccgtgg ataaataatg
     1201 tcctgagata agtgtcacca cgtttgctta tccccagcag gcgtacttta cctccggagc
     1261 cggtttgttt tggaaccaga ccgacatatg cggcgaactc tcgccctgat ttaaacgctg
     1321 atgcttcccc catggtggcc accgctgccg tcgcaataag tggtccaacg ccgggaatat
     1381 ccagcaaccg cttacaggtt tcattctgcc tcgccacgct ggtaagctgt ttttcaatat
     1441 cctctatcag taagtccagc tcattcagcc ggttgtactg gttttccagc acagtaatga
     1501 gatacggtgg cagtctctct ttcatccgct ccagtgcttc aggaaactct cgctccatcg
     1561 ctgcccgacc tttgtgaatg gtttcaccaa attccaggag cgtgccgtgc aatgagttag
     1621 tctgcatggt gcggaacttc accagttgcc tgcgggtccg gtgcaacaca agaactgact
     1681 gctgttcttc tgtttttacg gcaatttctt taccaggctg ctgaaccgcc atccagatag
     1741 cccgggcatc catcacgtcg ttcttgtttc ccatgacgaa tgccttaaca aagcgggcct
     1801 gtaacaagcg gactttatgc cccagctttg tcagctcccg tgcccagtgc tgagagcctc
     1861 cacaggcctc cataccaatc aggcaggggt tccggttgct gaaatattcc agaaaatctc
     1921 tgccgcgaag ttgcttatcc accacttcac ctgtgttctc atcaatgaag tgaacctgaa
     1981 tcagatgttt tgcgatatca acgccaaccg gtgtatattt catttgtgga gtctccagtc
     2041 tgagggagct ttctgcatcc cgtattgggc actatgatgc cggaaatctg cgaggctcca
     2101 cacctctctg attttcccct ttattcatca ctgtgcccca gatttaatgt tggggatgcg
     2161 ttcatctcat tcccatcaac agcgtgacct gccagaacgg cataaagggt aatggcggaa
     2221 gccggatacc cagccgccag agaatacgca gaagaggagg ggcataattg ctccgccaca
     2281 tctttttgct gtccatcagg gcaatggccc gggctttttt ctgctcaaag gtcacaggag
     2341 cacctccgtt ccatgatgta aacgtgacac agcttattgt tattgattat tattatcata
     2401 ctgtgattat gttatcacgc accggtacac gataacaata aaggagaaac cagatgaaac
     2461 tggatatatc tgtaaagtat ttactgaaaa gcctgatacc aagcctcatt attcttacag
     2521 ttttttatct gggatggaaa gataaccagg aaaatgcaag aatgttttat gcgttcatcg
     2581 gatgcattat caatgccatt acttttcctt tttcaatgag gataatacag aaaatggtaa
     2641 taaagattac agggaaagaa ctctggcaaa aagacttctt tacaaatcca gttggcggaa
     2701 gcttaactgc aatatttgaa ttattctgtt tcgttatatc agttcctgtg gttgccattt
     2761 acttaatttt tatattctgc aaagcccttt caggaaaatg aaagggcatc attatgtgtc
     2821 agataccaat aagtttattg acctgctcaa taaacttatc attaacaagc gcactcacag
     2881 ccgccataat aattgcaaaa cccagaatac ctacaggggt tcccagcatg acggaaaacg
     2941 tccatgctgt aactgctgaa gcagctcttc ctgctgccag tgattcaagt tttacaaaaa
     3001 atggacgcca gttgtccgtt tttacagcct tttgtaactc cacagcaaca tcataacgat
     3061 caataacttt tccggtaaaa ccaaatgcct tactgaacag cttaaaattc tccgccatat
     3121 ggacctgatt aatagcttcc agggctttag aaatggccat gcgatcttgt atgctatatt
     3181 tcttgttcag attattacgg aatttatcaa aagcgttcag tgcatcatcc acactcttaa
     3241 tctgtttccc ttgagataca gaagccagct ccttagcaag ttcggatgct tgtttaccga
     3301 actcatcata tatcgttctg tagaaatcag aggtgagttt tatggcatcc tttaccatat
     3361 ttatttcatc cctttttctt ttctcttccg ctattttctg attgatgtca gcaagctggc
     3421 tacggatatt ctctttttcc ttcaacaggg cattaagagc gtcatttgca tgcttttgtt
     3481 catttgttct ggcactgacg ttatttctcg ccgaatttac cgcagattca gcagaggtga
     3541 ttttatttct ggcatcaagc aatcgttgcc gtaacttatc ccattcagca acttcagcat
     3601 tataaatttt cttgtcacct tccacatcct ctgtcaccac cggattccgg tcattcagaa
     3661 tattgcgttt atagtccaga ccggaatggg tcagaagata attcaggttt gcacggttat
     3721 cgatgactgc gctggttgtg atttttatcg taccggcaaa tctcggatca ccgctcacaa
     3781 caatagaatg atttgttgat gaacgccccg ggaatttttc aggggaaact atcgttttgc
     3841 cgtcaggtgt gtttttctgt tgcgtcagct gttgttcagc tctggttaat gcattacggg
     3901 ccgtgttaag cgattccgtc accgaactga gcttattccg ggcctctgaa atggccctgt
     3961 cattccgtgt ctgctgcccg agcagacggg cctgttgtgc atcaagaacg gcaagggctg
     4021 ccccgccatt tttttccagc tctgacaacc gcgtgtccag aatacctttt ctgcgctcgt
     4081 attcagcaag catatcagcg gccttcttgt ctgcgttctg cgcatccttc tgccgttttt
     4141 cagcctgttc gattaacgac ctggcctccc ggggagatat acgaagagaa tcagcatcag
     4201 caaacagcat ccggctctct gtatgcagcc ggagggcgtc atatccggcg atttcagtac
     4261 ggaatccgta ttctctcagc tctgcctgca ggagtctgaa ctcctgtcgt gtaatatcag
     4321 cggcatctgc gtttccggca cggagtgttt tcagtgtgtt ttcatctttt tcccttgcag
     4381 caatcgccgc agagaggcgt tttccggctt cagtgcgctt attctcattt tctttctgct
     4441 gcgcgctgag cgtgtttttg tacgctgtga tttccttgtc ccttttttcg atatcacttc
     4501 gggttgggga atcatcgacc cactcgtttc cgccccagat ggttttgttc ccgaaggaac
     4561 tccatgcagg cggggtacca tgaaagacat cgacacgtgg agggtttaca taaatatcaa
     4621 cggccataat ttcatggcca tctgaatcat accccagcga ttctgcaccg ggatttgtaa
     4681 tacgtacagg gtcagacatt cggtatctcc ttcatcctct gaggaaaaca aatgaattaa
     4741 ctgcttacgt atatggatac atatacagca ttaatttatt aaggagaaaa tggcatgtca
     4801 agatttaatg atagtgatta tcattaacat gaagtgatgt tacaccgttc agaaagcctg
     4861 ctgacgctgt ggcagcatca cagtctgagt attctctggt gcaatacaaa aaccggactt
     4921 tcccgtaaca acagacaatg ttacgggaaa agttacagaa ctgagccaca aaatatctca
     4981 gtacctgaga tgcataaaaa caccgtacat atgtttctca aaagggggat gatcgttatc
     5041 cagccatttc ggtggatcgg tatgcagaac accaatctgc ttcatgccgg tatgctgcct
     5101 ggattttctc gcgcagtaat cactgtcttc atatcccatc gtttcgatga ccactctggc
     5161 cgtctttccg tcaggagccc ttgctgtgac aatgaaatcc ggcaggacat atttcttttc
     5221 accattaaca taaacttctg tatcaaacag gggcctttcc agacttaatt ccggtgtacc
     5281 tggctttctt ttaagccagc tggccacatt cagaagagca gtgagcgtgt ttcgttccag
     5341 tttgctgtcc acaggcaccg gacatgtcag ttgataaaga gcatgagcat acccttcact
     5401 gcagatgatt ctgccatcgg cactccggca aaaagcaaga ataacccagt aagggggacg
     5461 tattccgccc tgcgcttcac cattaatact gatcccccgc acaggtctga tgacctgaat
     5521 attaccattc gcccagtgaa tttccacagc atcacgacag atgtgctgcg ccataaaaat
     5581 ctgaaagaac atatgttttc ttctggcagg ccagtgtcgt tctgaattct ccagcgtttc
     5641 cattaaccgc tcctgagacc tttcagacat tccaggccgg aaatggataa tttcagataa
     5701 ggctcttccc cgaatatact cctgctgccg tgtaacctgc cgtatggcat caagacactc
     5761 acgataatta gtccggatac ggggattagc cagggaatcc agtttattca gcccagcatc
     5821 gtcaataaga gacaggagta aacgggcaag gcgcggacgt ttcttacgaa cggaggagct
     5881 ctcaccatta tggtaagcag agcgggcctg cagtctgata acagaattac tgtcatcagg
     5941 tggtaaaaaa tttctgtaat ttatacgggt gctaacggga tgccggcttg ctccggaaga
     6001 tgtttcctct tcatttccgg ataactgacg atacatgggg cattcaggtg catgctcatg
     6061 attaaaactg gacagataaa gagtacccgt attgttttta agagcagcaa aattcatggc
     6121 cggcgaatct cccggaatac agtcacactg taaccatgca tcatactcac gcatttcccg
     6181 gagaatatca ttggccaggt tatgtagtgc catggttgct ggctttttca gtccacagac
     6241 cgtctttact gcagtctctt cctcactgct aagctcccga atcactttgc cccggggatt
     6301 cttctttaca agtatcattg gcataatgaa agcactcctt atgtacatgc ttaaacagta
     6361 agtaaaatat acaaagaaca ccccatgaaa caaaggcaga gatgaactgt gcgccccgat
     6421 atcttcttct gatatccttt caaatgaatt gtttttattt atatcatgac aacattaaag
     6481 tgagagaata tatcaaccat gtttctgtca gggttcagta gaatatcaat tcgactgtca
     6541 cgccataact gttactacct cgagtataaa tattcagaga acggacttat tagccatgaa
     6601 aaatcacctg atggaaaatt tactaattta tgaattgtga tattattaaa gagtaaaatc
     6661 agccagccga ggtatttatg ggaaaaaatc aggaagaaag gaaaactccc gtcattgtgg
     6721 tgaaaaaacg tcgtactttt tcgctaccgt ccttatccga aaaaacagat attatcgcac
     6781 ctgtatttac tgaacagacg gcagaatcag cccccgccgg aataaattca tctgccgtcg
     6841 aaacacatat ccccgaagcg ccagccagga agaagaaaaa gaaaagacac cgttttccac
     6901 gtccctccca ctggacccgt gagtatactc acgagtgcgt ggaaaaaata aaggccctgt
     6961 ttcctcatct tcgtgctgaa ggtggtggat ttattcccct gaaaattggc ataaacaatg
     7021 atatttcggc atttctggca gaacacccgg agacagaact gaccatggac gaatggctct
     7081 gtgcagtgtc ctgcatcaca tcccgccgcg tatatctgca gcggacagct gtcgccggcg
     7141 tcccacgtta tggcctggat ggtcacccca aagggcaggt ctcagactct gaagcacaga
     7201 gtgccggtcg cagactggcg acattagaac aaaagtggct caggatgcag gcacagcagg
     7261 agaatatttc tgggcaataa acactcctga taacaggtgg acattacgta tcagaaaaaa
     7321 tgggaagaga atgttatgtg tgattcagaa gcggagaaga ttttattcat cctgaataaa
     7381 catgaacggg catacagatg ccagtacccc gtatatattt ttttagctat agtattatgg
     7441 attttaccta ttctcctgat tatatacctg catttctgta taggaatcgg tttctgtatt
     7501 gcatggacag catactactt tctttcgctt ttcgagtatt ttgtaaaata cttctttcct
     7561 ctcgatcgag aagatttgct taacctgatg gatctcgcca aagattctcc tgatgcgaaa
     7621 caggagcttc ttgagcgtct tttgtccgga aaaagaatga ccgggcggga tgaaatggat
     7681 atcaagcgca ttctaaaaac aaaggaagca cttgataaca tcgggaattt tattgataaa
     7741 cagcagttaa cgaataatga tactccgaag aaaagcacgg tcccaaatga agataaaaaa
     7801 ggcataagat gatgatcaca ggaaatggaa taaacactgt aactgtcaat ggaaaagtga
     7861 aacacatcac agaactggat tatataacac tttgtttaga gtggacaaaa ctacgagagg
     7921 aaaacaatcg tctttatgaa attaataacc aggcaaacag aggatggcgt ggtttcatcc
     7981 taagattgat aggggttaac ctgccagata aaaggactga gtttacacga cgtctcttat
     8041 tgacgcgcaa tatttcgggt tcggtcatga aaaagtaaca tattctatgc tggaacagac
     8101 caattttgca ttcgccgtcc aggagtttga ggggaaagta gtcagtgttt acgctcagtc
     8161 agggaaagtg tgcaatgata aacaacacga aaggaatcgt agtacatggg caccgcgatt
     8221 caatattcaa ggaaaacaca atacagcaat gtacttgtac tgtttctatt attctggcga
     8281 tttcaataaa ggcacttgct cgcgctctgt cattttctga aactcttcat gctgcatttc
     8341 gattttcctt ctaagatcat aactcgcata cctgttcttt ttatgtatat ctatatctat
     8401 ttttgtgacg agttcaaacc acaatgttcg cactccccag gcagcgttat gaaacatttc
     8461 cgcaacggct atagagtctg tattaactgc ttcggtcatg tagtcgctaa tacgttcagc
     8521 ggtaattttc gttatttcat cgtaattcat tttatctcct acaagattat ttaatggaat
     8581 gaaaaatatc tggcctgata ataatgttcc agaattaata tctgcaggct atgttaagtt
     8641 gtccccacac acaatctacc tgttttctcg tgtgtgccac aggcgcaata cataaatagt
     8701 ctggccatta agttcgtaac ggatttcgta ctcgccagca aaaatccgtc tgacttccct
     8761 gggttcaaac tgaaacaact gttctcccat acgtggatga gttaacagaa tgaccggtgc
     8821 ctgtgtcagg gattgcaccg ttctggcggc cgcaggttta ctggccagca ccagaaaatc
     8881 atataaccgc gccaaatcag aaagcgcctt actggtccac ttcagttcca tcagcgtggc
     8941 accggtaacg gattgtcagt actgaggctg tccgcccagg cctgtacagc ctgatggtcg
     9001 ataacctgtc cggatgtcac atcgtccagg gcttccagcg tcaggcgatt acgctcctcc
     9061 tcctgggcaa gccatgcaga aagcgcctgt ttgataaccc agccccggga gcgttccagc
     9121 ctggcggcca tctggtcgac tttatcagcc ataggtaatg gcacatgcgc ggtaatgact
     9181 tttgttgcct gtgcggtatt gtttttcatt tgcatatccg attagttgtt aatcatattt
     9241 aatcatagtg attactttca gacggaacaa ggcaaataat gcgcgtcagc gcatgtaagg
     9301 cggcatacgg tggttacatg tagcacaggt gtgaaaccgc cgaagcccgg cgtaagccgg
     9361 tacggattga tagatttcac cttacccatc cccagccctg ccagaccata cccgctttca
     9421 gccatgagag agcttctgtg cgcggtcgga gtggtcccga cgagggttta cccgaagtcg
     9481 gggcgtatct ccgcgttagc gggccgtcag ggccgcttac gagcgtgtac tcgatacttc
     9541 cagccagaac actgacagcc atgatgaggt gtagttacaa cattcataat taaaagcgat
     9601 gttgttcccg ccctttgggc gggggcggcc tcgcttttca gttatgagag gtgagcacgg
     9661 tgctgcgttt gtgtgatgtt tttattcctg atgtcttttt gctgtggttt cagaacggtg
     9721 aggttcgggg cccgcgcgtt agcgtgggag gtgcatattt gcttgtccac aggacaggca
     9781 ctgaataggt tttcttttca aatggaagta attaagtagt ttaaaggaaa tacagtactg
     9841 gggtttaaaa gaaacattgc accctgtaac gcctgcggct ggcgctttat agcattaacg
     9901 gttgtaacct tacggagaag tcccttgcag ttaaatgtgg ataaacagaa ttccccgtct
     9961 gtgagacgtg ttttgcgtga aaaacagggg gagcaaggcg agccggaagg cagatgatga
    10021 ggctgttttt tgtgtgctgt gctgcttttt tgtgcgtcgc cgggcttcag acgtgcggaa
    10081 gcctccggcg caggccggat tattcagatg aggtcaaact cagggtgagg cagtggccag
    10141 ccgtgtgtag ttgttgttcc gcgacattat catgcgttcc ttcacgcgac ggtcgatttc
    10201 gcgcttcacg gcctccaggc ttccgcggaa acgcccttcc gcgatttcac gtgtcagctg
    10261 ccgtttcacc agggtgacaa tgtcctgacg ggtcctgcct gcatcgcggc gcgcacgggc
    10321 acgtttcatg ccgtgggact taagctctgc ctgataacta cggaaacgct cgcgcacaaa
    10381 acgccaggct ctcgctatca gttcatccat acccagcgca tccagcccct gctttttgcg
    10441 cagcttgttt tcccattcca cgcggctgcg gcgtgcggaa gccaccgcct cttctgatat
    10501 atcgagtgag gaaaacagtg ccggcgtgaa cgtgatgtcg gtcgggatgt tacagccaat
    10561 tgtcgggtcg tattccgtct ggtaggttat cagccccagc tcagacagga acttcagggc
    10621 acgggtggca cgggtgatgg agagtttccc ggcctcagac tccgtcgcca gtccgcactc
    10681 aatggccagc gtggtgatgg agcactggac gcggttggcc agcggatcat agtggaaaca
    10741 cagcccctgc agcagcgcat caatcgcccg gcggcgcagc accggcggca tacgccggcg
    10801 caggccgcgc gaacgggcat acgccacatg cgcggcaaaa tcaaaacggg aggtgaagcc
    10861 caccgccttt tccatcagtt tttcgcagaa cttcagcgtt ccggcatctt cacggggtgt
    10921 gaacaccgga ttcgggttct ttacctgacg gtatgtgctt tcagtaaatg aggtctggtt
    10981 cagggccgcc acaagctgtc gcttatggcg gaaacagaat gccgtgcgtt aatacactgc
    11041 gaagggttga atctctgaca cggtttcata tatacttatc ccgtattctg tgaggccccc
    11101 ataatcttct gtgcgttccg ccaagttcga caggaagatt atgggggttt tcctttatgg
    11161 ccttctgaaa cttttttcag ccttccggcc ccggcaaact gccgtcgttt ctgccataca
    11221 tcctgaatgc cttatggcgc attctgccgt tttctggctt tccggaccgc gaggatccca
    11281 aaagccactg aataacgatc cgcaatatac cccttttcca ccgctttgaa aagccgacac
    11341 gatttctctg atttaccagg cctctcatta accacctaaa aaacgtcgtt tttttgaact
    11401 atgtcaaaaa aatagacaat tccacaaacg atcttttatg ctgtgttttt catcattttt
    11461 agtagaagag atcgcctaaa atttttaaac gatctaatcc tccagacacc gcacacaaat
    11521 cagcataata tgctgcttta tcaaatataa cgatctattt aactgcttat cttcactctt
    11581 gccacagata catccttatg taatatattt gccagccatt aatcctgttt gcaggagttg
    11641 tcggttttcg cccgcctcga aaccgtccac aaggcttaca agagtgcgta aatcatgtgc
    11701 ctgtcagaac tgcaggccgg gctgaacgag ggtcagccgc tgtttccgac acatgcttta
    11761 tgtattcgcc gttcacaaca acatcgaagc ccctctgcgg ggatcgattt tcgcggagcg
    11821 ttccggattt tgaatatcag tgaattacat caacgcaact ggtatctggc tcagtacatc
    11881 cctgccggca aaaaccgcga acatctcttt tcctggcttt ccgaacaaca cgtccttccc
    11941 tggacacccc tgatactgaa aaaggtccgc cgcacggata aagtctgtgg ttaccgtcga
    12001 catatacacg ccgtgtttcc cggttacttt ttcctgaaag cagaccccga acgccacagc
    12061 ttcactcacc ttcgccgtca cagtgccttt ctggacttcg tgaaaatggc cggtgaaatc
    12121 aaaaccgtca gggaggatat cgttcagtct ttgatgaaag tgtacccgga tcctgcgctc
    12181 aatccggcag ccagggagga gctggatgct gcctccaccc tctggctgac aaaagcgcgt
    12241 taccagtacc tcctccggct tgatgcacag cctctgccgg agtcgaggat cgccctgctc
    12301 ctgcacctgg tatctgatga cggagcgttg acatgaaaat tgctgtctta atttgacgac
    12361 ttttcccggc ctttatggcc gggatttttt tcccttctgt ttccacaatg cccggcaccg
    12421 gtgacacctg agggtgtacg gagagcggct gtcgggcatt ttttcaggac gcctttatga
    12481 ccattctatc ccaggaaaac cctgtcattc tgttactgga gaatatgctc gaagcactct
    12541 ccaccgcccc ggacaacata aacaacgaac ggcgtcgccg gcgttacctg ctgaactggc
    12601 ttgacactgc ccgtcagatg cgcgagttca gaggaatggc tgaagagttc actaccctgc
    12661 gaaaactgct ggccaccaat tcagacatac cggtagcaaa caccctgaaa tcactgctgg
    12721 aaagtggcaa taacgcagca ttatacgacc tgtaccgctt ccgctcggct ctttccgcgt
    12781 gcagaagtct gggatggcag attggcacgt gcgcatggaa cgaccagctg ctgagtgaga
    12841 cactttcccg tgcgcaaaca ggtaaacgtc atatcctgca gttgaacagt accagagaca
    12901 ccttttccac cacgggcaga atgctcagac cggtggcatt ttcattaatt catccggcat
    12961 ccctcgaatc ttcagaagtg gaagagatat tccgggatga aggttttata ctcgcgcacg
    13021 gacgcgaatg cagcctgaac ggcagcggca gaaacatgct gtcacgcatt cttcatgtgg
    13081 ggcattcagg tttaccgaaa gccgaatggg gcatagatca cagcaatcac ctgttacact
    13141 gaaataccgg gacagccaca acggcagcca ctctccgctg actttttttc gcaaatcgcg
    13201 gtttgctgat gtggatgtcc cattttttat tatccgtcct ctccggttgt cataaagcgc
    13261 attccggcgt gaggcttctg ttgttatcat cccctcctgc agacatcccc ttgtcttctg
    13321 atgactgacg atatgcttct tctgacaatt aaagaagcgg aagcttcacg ccataatgtt
    13381 gtttgactga cctgccacag gaggaaaaat gacactgacg ttacttaccg cgtataacgc
    13441 accctatctg gcagccctga cttttgttct gcttactggt atcgctgaac ttatcgccct
    13501 gctctgcgga cactccctct cttcagctat ggatacacct gatttaccgg aggggctgac
    13561 aggagaggcg ctggactggc tgaacatcgg acggatcccc ctgctgatcg tcctctgtat
    13621 gctggcaggt ttttttggta tatccggcat tctcctgcag ggtcttatta ttcatctgct
    13681 gcaggctcct gccccgaaca ttctccttgc accactttgc ctgctgctga catgtcctct
    13741 cgtacaccgc accggcagac tgattgcccc ctggttaccc cgtgatgaaa caacggcgct
    13801 caccgaggag gcattcatcg ggtgtatggc caccataacc ggttatcacg cagtggcggg
    13861 ttccccctgt gaaggtcgct tcacagaccg ctttgggcag gttcattacc tgttgctgga
    13921 gccggaggcc gggaaagaat tcagacgagg agacaaagtg attattgtct gccgtctttc
    13981 cggttcccgc tggctgggcg aactgaatcc ctggccggat gttctttaat cccgacatat
    14041 acagaggttc tcactatgtt taatcttccg ggatggttgt ttaccgttat ttcgctggtc
    14101 ctcaccctgt tcgttatcgg gctgatattt gcccgcctgt accgtcgggc atcggcagag
    14161 caggcctttg tccgcacagg tctgggaggc caaaaagtgg taatgagtgg cggtgcaatt
    14221 gttatgccca tttttcacga aacaattccc gtcaacatga acaccctgaa gctggaagtc
    14281 agtcgtgcag cggctgaaag tctcattacc cgcgaccgca tgcgtgttga tgttgccgtt
    14341 gccttttttc ttcgggtaaa accatcagcc gaaggaatat ccactgcagc ccagacgctg
    14401 ggacaacgca cgctcacacc tgaagatctg cgtagtctgg tcgaggataa atttgttgat
    14461 gccctgcggg ccacggcggc cagaatgagc atgcaggatt tacaggatgc ccgcgagaac
    14521 tttgttcagg gtgtgcagaa caccgttgcg gaagacttat ccaaaaacgg tctggagctt
    14581 gaaagtgtgt cgctgacaag tttcaaccag actgcacggg aacattttaa tcctgacaat
    14641 gcctttgatg ctgaaggcct gacccttctg acccaggaaa cagaacgtcg tcgccgcgaa
    14701 cgtaacgaag ttgagcagga tgttgaagtg gccatcaggg aaaaaaaccg ggatgcactc
    14761 tcccgacgtc tggagattga acagcaggaa gccttcatga ctctggagca acaacaacgg
    14821 gtaaaaaccc gtacggcaga gcagagtgcc agtattgctg ctattgaagc tgaacgacgt
    14881 cgtgaagcag aatccgcgag aattctggct gaacgtaaga tagaggaagc cgaaatcgaa
    14941 cgacagcaga tagtgcgtac ccgccaggtc gaagccgaac gggaagtggc catacgggaa
    15001 attgagcaac agcaggcaac cgaaatcgcc agccaggcca gagccattgc cgtggctgca
    15061 aaatcggaag agcagtcaca ggcagaagcc cgggcaagta aagcactggc tgaagccgta
    15121 caggcccagc aggatgtgga aaccacccgc cagactgcgg aggccgaccg taccaaacag
    15181 gttgccctga ttgctgctgc tcaggaagct gaaacgaaag cggttgaact gacggttcgc
    15241 gcacaggctg aaaaagaagc agccgaactc caggcagcag caattattga actggcggag
    15301 gccacccgtc agaaaggtct ggcagaagca gaagcacagc gcgcactgaa cgatgccatt
    15361 aacgtgcttt cggatgagca gaccagcctc cgcttccgtc ttgccctgct ggatgcatta
    15421 cctgccgtca tagagaagtc cacagaacca atgaaatcca ttgacggcat taaaatcatt
    15481 caggtggatg gcctgaatgg cggtgttacc cgtggtaaaa cagcaacaac cgacataagt
    15541 ggcggaaatc tcgctgaaca ggcggtaacc gccgctctgg cttaccgtac acaggctccg
    15601 ctcatcgact ccctgctgaa ggaggtgggg ttatccggtg aaggacttac agccctgaca
    15661 cgtccactgg tcatgccagc cccggaaaag gtcccggatg tcagcgaacc ggtaaccgct
    15721 ccggataact cagaaccaca acaggtttaa tccccgtgcc ggaatttttc cggcatcttt
    15781 ttcacaaaga atgccctcac gggatattct ggtttaaaag aggattgaaa atgtccgcag
    15841 aaaaattcag agttgtcata tacaccaata catgtgaaaa gaaagtcagt tatattcctg
    15901 caacagaaga tataaacatc gctataatgc catttcagac ggcagacaat ggcttaatta
    15961 ttgtgtgtca gaactcccgt ataatacgac gcaaagagcg tatacccgga agcagctgtg
    16021 aatggaaaga tgtgacatta gcaccaaaat ccagaaaccg cgttcagtta tagcctgaca
    16081 gcctgctttt tatccctcag aaaaggggga taaaaattaa atatttcaca accgagatat
    16141 ttttcaggaa tgcagattat tttatgttat gaccaacaga gaaacaggca tgagaacaat
    16201 cacaacgcat gaacaattat taattaatgg gagagtccac gaaagaatcg ccacacatat
    16261 agttacagga gctcatgggt atgaaacgct ttgcacaaat ggctataacg tcagatacaa
    16321 caaggcgcag caactggttg aaagctgcga aaaaatagct gaaggcaaac tacctgttac
    16381 ctgtcctgtc tgtttcgcca tatggcagga tgtacacagg ttcacccacg ttgactttga
    16441 cacacaatcg gggaaaagtg attttattga tacctgccat tcggagatca taaccggaaa
    16501 tgtttcaata acaggcaaga aataaaataa tgctccagtt ctgaaatgca attcactctt
    16561 ataatccccc cacgaacatg aacgttcatt atcaataacc cgatgaaatg atattcatct
    16621 gaaggccagt tacccgccac ctgtcttata ccgtaacccg taatataccg gattttattc
    16681 tatgcaaaaa aaatcactta cactccttct gccgtcatta ctcagcggct gtacggcatt
    16741 taatgccacg cctgttcctg acaccacacc tccggctctg gtgttcgtcg acggacaaat
    16801 tcgcgaaagc gctgccatta tcgcccatac gcaaaaacgt ctgct
//
LOCUS       DAZFXT010000102        16803 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.102, whole genome shotgun sequence.
ACCESSION   DAZFXT010000102 DAZFXT010000000
VERSION     DAZFXT010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.102"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(186..1379)
                     /gene="tyrB"
                     /locus_tag="ACHKW0_003569"
     CDS             complement(186..1379)
                     /gene="tyrB"
                     /locus_tag="ACHKW0_003569"
                     /EC_number="2.6.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HFO7476336.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(1632..2711)
                     /gene="alr"
                     /locus_tag="ACHKW0_003570"
     CDS             complement(1632..2711)
                     /gene="alr"
                     /locus_tag="ACHKW0_003570"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HFO7476337.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGIIKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(2764..4179)
                     /gene="dnaB"
                     /locus_tag="ACHKW0_003571"
     CDS             complement(2764..4179)
                     /gene="dnaB"
                     /locus_tag="ACHKW0_003571"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HFO7476338.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4262..5245
                     /locus_tag="ACHKW0_003572"
     CDS             4262..5245
                     /locus_tag="ACHKW0_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HFO7476339.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(5411..5653)
                     /gene="pspG"
                     /locus_tag="ACHKW0_003573"
     CDS             complement(5411..5653)
                     /gene="pspG"
                     /locus_tag="ACHKW0_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HFO7476340.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(5787..6824)
                     /gene="dusA"
                     /locus_tag="ACHKW0_003574"
     CDS             complement(5787..6824)
                     /gene="dusA"
                     /locus_tag="ACHKW0_003574"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HFO7476341.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(7187..7894)
                     /locus_tag="ACHKW0_003575"
     CDS             complement(7187..7894)
                     /locus_tag="ACHKW0_003575"
                     /inference="COORDINATES: protein motif:HMM:NF022352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HFO7476342.1"
                     /translation="MWVNKYIDDCTDEDLNDRDFIASVVDRAIFHFAINSICNPGDNK
                     DATPIEQCTFDVETKNGLPSTVQLFYEESKDNEPLANIHFQAIGSGFLTFVNACQEHD
                     DNSLKLFASLLISLSYSSAYADLSETVYINENNESYLKAQFEKLSQRDMKKYLGEMKR
                     LADGGEMNFDGYLDKMSHLVNEGTLDPDILSKMRDAAPQLISFAKSFDPTSKEEIKIL
                     TDTSKLIYDLFGVKSEK"
     gene            complement(7918..8172)
                     /gene="yjbL"
                     /locus_tag="ACHKW0_003576"
     CDS             complement(7918..8172)
                     /gene="yjbL"
                     /locus_tag="ACHKW0_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjbL"
                     /protein_id="HFO7476343.1"
                     /translation="MLKIIPGATGYFNKTLNSNQFDNEDTIKDKLDNRGSIKGKLNNI
                     YGKSIDYAALRHRDIIIAKIDLFIQRITHNLWHARKKMCF"
     gene            8490..9005
                     /gene="zur"
                     /locus_tag="ACHKW0_003577"
     CDS             8490..9005
                     /gene="zur"
                     /locus_tag="ACHKW0_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HFO7476344.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(9047..9256)
                     /locus_tag="ACHKW0_003578"
     CDS             complement(9047..9256)
                     /locus_tag="ACHKW0_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HFO7476345.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(9372..10697)
                     /gene="dinF"
                     /locus_tag="ACHKW0_003579"
     CDS             complement(9372..10697)
                     /gene="dinF"
                     /locus_tag="ACHKW0_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HFO7476346.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIVAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(10770..11378)
                     /gene="lexA"
                     /locus_tag="ACHKW0_003580"
     CDS             complement(10770..11378)
                     /gene="lexA"
                     /locus_tag="ACHKW0_003580"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HFO7476347.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(11488..11856)
                     /gene="dgkA"
                     /locus_tag="ACHKW0_003581"
     CDS             complement(11488..11856)
                     /gene="dgkA"
                     /locus_tag="ACHKW0_003581"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO7476348.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            12027..14450
                     /gene="plsB"
                     /locus_tag="ACHKW0_003582"
     CDS             12027..14450
                     /gene="plsB"
                     /locus_tag="ACHKW0_003582"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004366 - glycerol-3-phosphate
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HFO7476349.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(14605..15477)
                     /gene="ubiA"
                     /locus_tag="ACHKW0_003583"
     CDS             complement(14605..15477)
                     /gene="ubiA"
                     /locus_tag="ACHKW0_003583"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HFO7476350.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(15490..15987)
                     /gene="ubiC"
                     /locus_tag="ACHKW0_003584"
     CDS             complement(15490..15987)
                     /gene="ubiC"
                     /locus_tag="ACHKW0_003584"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HFO7476351.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(16210..>16803)
                     /locus_tag="ACHKW0_003585"
     CDS             complement(16210..>16803)
                     /locus_tag="ACHKW0_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO7476352.1"
                     /translation="KDPIIRSYIHSGEDNKFDDFMRQHRFSKVDFDTQQMIHFINRFN
                     MNKGLIDKNNNFFIQLIDQALRSTDDMIKANAWYLYKEWIRSDDVSPLFIEIEDNLRT
                     FNTNELTRKDNIFILFSSADDGPVMVVSSQRLHDMLNPTKDTNWNSTCIYKSRHKMLP
                     INLTQETLFSSKSHGKYALFPIFTASWRATRIKNIGI"
BASE COUNT         4374 a         4419 c         3974 g         4036 t
ORIGIN      
        1 cacccaaaaa aacaggataa ggaattccag ataattagga taagcttaac aataaaagaa
       61 ctaataacgt ttattatctc tcacgtagaa cgatggcatc aaaaaaatca tctataaaga
      121 aagagtgaaa gcgacgacag tttaatttca ctgcaggctg ggtagctcca gcctgctttc
      181 ctgcattaca tcaccgcagc aaacgccttt gccacacgtt gtacatttgc cgtatttaac
      241 ccggcgacac acatgcgacc gctggcgatg agatagacac caaattcttc acgtagtcgg
      301 tcaacctgag cggcacttaa accggtataa ctgaacatgc cgcgctgatt aagcagataa
      361 tcgaaattgc gttctggcat ctctgtgctt aataccttca ccaattcctg acgcattgcc
      421 agaatgcgag tacgcatctc ttctacttcc gccagccagc tggctttcaa tgcctcgtca
      481 ttcagcactg cagccaccac ctgcgcacca aaattcggcg ggctggagta gttgcggcga
      541 actgttgctt tcaattgccc cagtacgcgg ccagcggctt cggcatcttc acacagaaca
      601 gaaagtccgc cgacgcgctc gccgtaaagg gagaaaattt tcgagaacga attgctcacc
      661 agagcgggta atccagcgct ggcaatggcg cgaatagcgt aggcatcctc ttccataccg
      721 gcaccaaatc cttgataggc aatatcgagg aatggaataa gctcgcgggc tttgagaatt
      781 tcaatcaccg catcccactg atcattagtg agatcggcac ccgttgggtt gtggcaacat
      841 ggatgcagca acacaatact gcgggcaggt aatgttttca gcgtcgccaa caggtcatta
      901 aagcgcacgc cgttagtcgc ttcgtcatac caggggtaag tactcacttc gaatccagcc
      961 ccggcgaata ttgctacgtg gttttcccag gtaggatcgc tgacccagac gcctgattcc
     1021 gggaagtagc gtttcaggaa atccgcgccc actttcaatg cccctgagcc gccaagggtt
     1081 tgaatggttg ctacgcgctg ttgttgcagt actggatggt ccgcaccaaa cagcagcggc
     1141 gcaatggcat ggcgatagct gttaagccct tccatcggta aataaagcga agcgccatga
     1201 ggctgcgcat tcaggcgcgc ttccgcctcc gccacggctt gcagttgtgg aataattccg
     1261 tcttcgttgt agtacagacc gatacttaaa ttcactttgt cgctgcgagg gtcttcttta
     1321 aaacgctcca taagcgtaag aatcgggtcg ccagcgtagg cgtcaacttt ttgaaacacg
     1381 cgatggttct ccaggtttac gggcaggtgg ttaaaacaca ataaaccgga agaaggcgaa
     1441 gatcgagtgg atgttcagga gcgaacggca attagcaaca gagtgagact catgacaaac
     1501 gtacatccgc cagaggcacg acctttataa gagcatgaaa gaacatcaac ttattgaatt
     1561 tttaggattt tattggccgg ataaggcatt cacgccgcat ccggcacaga caatcaaata
     1621 ttacagaacg attaatccac gtatttcatc gcgacccttg aagtcaggcg cgtaataagt
     1681 tcgtaagcgc ttactttcgt catttcagcg atacgttcta cgggcaaacc ttcgccccat
     1741 aaaatgaccg gatccccggc tttgtcctgc gcttgtggac ctaagtctac gcagatcata
     1801 tccatcgcca cgcgcccgac aatcggtact tcgcgaccgt tcaccagcac tggcgtaccg
     1861 gacggcgcgg cgcgcggata accatcgcca tagcccatcg cgactacgcc aagacgggta
     1921 tcacgttcgc ttacccaggt tccaccataa ccaacaggtt ctccggcttt atgctcacgc
     1981 acggcaatca ggctggaggt tagtgacatc actggctgac agccaaaatc ggcaccggtg
     2041 gagcgatctt ccagcggcga gacgccataa agaatgatgc ccgggcgcac ccagtcaaaa
     2101 tgcgactgtg gccacagcag aatgccaccc gatgcggcaa tggaacgttg accaggtttg
     2161 ccttcgcaaa aggtattaaa gatagcgagt tgtttctcgg ttgcgccaca ttttggttca
     2221 tccgcgcgcg caaaatggct gacgatattc accggctgac gaacgttttt gcactgggtc
     2281 aggcgatgat aaaacgcctc agcctgttcc ggccttacgc ccagacggtg cataccggta
     2341 tcgagtttca tccagacggt aaccggctcg tccaggctag cctcttccag cgcagccagc
     2401 tgttcttcgt tatgcacggc ggtatgaaaa tgttgcgcag aaatcgtcgg cagatctctg
     2461 gcatcaaaaa agccttcgag taacagtaca ggtttgatga ttccccccgc acgcagtcgc
     2521 agagcttctt cgagacgggc tacgccaaag gcgtcagcat cggggagcgt tcgcgcggtc
     2581 tcaagaagac cgtgaccata agcgttcgct ttcaccaccg caaccatttt actggcaggg
     2641 gccagttcac gaagacgttg caggttgtgt cgcagagcgc ggcggttaat cacaacagtt
     2701 gccgcttgca tttgtgttcc ttgataagtg tttgctttaa ttacctaatt cataaaataa
     2761 ttattattcg tcgtcgtact gcggccccgc atagttgtcg aagcgcgacc attgaccgtt
     2821 aaaggtcagg cgtaccgtcc cgattgggcc gttacgttgt ttaccgataa taatttccgc
     2881 gatgcctttt aaatcactgt tttcgtgata cacctcatca cgatagataa acatgatcaa
     2941 gtccgcatcc tgctcgatag agccagattc acgcaggtcg gagttgaccg ggcgtttgtc
     3001 ggcacgttgt tccagagaac ggttcaactg ggacagcgcc accaccggca cgttcagttc
     3061 tttcgccagt gctttcagcg agcgagagat ttctgcaatt tccagcgtac ggttatcgga
     3121 aagcgccggt acgcgcatca gttgcaggta gtcgatcatg ataagcccga tgccgccgtg
     3181 ttcacgggca atacggcgtg cgcgggaacg cacttccgtt ggcgtcaggc cggaggagtc
     3241 atcgatataa atattgcgtt tttcgagcaa aatacccatg gtgccggaaa tgcgcgccca
     3301 gtcttcatca tcgagctgcc cggtacggat tttagtctgg tcaacgcgcg acagcgacgc
     3361 cagagaacgc atcatgatct gttctgatgg catctccagc gagaagataa gtactggttt
     3421 atcctgcaac atcgccgcgt tttcgacgag gttcatcgca aatgttgttt tacccatcga
     3481 cggacgcgcg gcgacgatga tcaaatccga cggctgcaag ccagcggttt ttttgttgag
     3541 atcgtcataa ccggtgttta ctccggtaac gccatcgtgt ggctgctgaa acaactgctc
     3601 aatacgcgcc acggttgcgt cgagcacatc ggcgatgttc ttcggccctt cgtctttgtt
     3661 tgcacgactt tcggcaattt taaagacgcg ggattcagca aggtccagca gatcttcgct
     3721 ggtacgcccc tgcggatcaa aaccggcttc agcaatctca ttcgcaaccg agatcatctc
     3781 acggacaacg gcacgttcac gcacgatgtc agcataagca ctgatgttcg ccgcacttgg
     3841 cgtatttttt gacagctctg ccagataagc aaaaccaccg acgctatcga gttgcccctg
     3901 gcgttccagc gattccgcaa gggtaatcag atcgatagga ctaccgcttt cctgcaaacg
     3961 cgccatttca gtaaagatat ggcggtgtgg gcgggtgtaa aaatcgtctg ccaccacacg
     4021 ctcggctaca tcgtcccagc gttcgttatc cagcattaaa ccgcccaaca ccgactgctc
     4081 cgcttcgatc gagtgcggag gcactttcag cccggcaact tgtggatcgc gttcgcgggg
     4141 ttcagcctgc tgtttgttga agggtttatt tcctgccata gtgaatggag ttaccgagat
     4201 aaagaatggg tcgaaacttt accatatgaa gcagaccctg acgatacgtt ctggaggaca
     4261 catggcaaca cgaattgaat ttcacaagca cggtggcccg gaagtacttc aagccgtaga
     4321 gttcactcct gccgatccgg cagagaatga aatccaggtc gaaaataaag ccatcggcat
     4381 caattttatc gacacgtata tccgcagcgg cctttacccg ccgccatcac tacccagcgg
     4441 attaggtacc gaagcggcag gcatcgtgag taaagtcggc agtggtgtaa agcatattaa
     4501 ggcaggcgat cgtgtagtct atgcgcagtc ggcgttaggc gcttacagct ctgtgcataa
     4561 cattaatgcg gataaagcgg cgattctgcc tgcggcaatt tcttttgagc aagctgcagc
     4621 atctttcctg aaaggcttaa cggtttatta tctgctgcgc aaaacctatg aaattaaacc
     4681 cgatgagcag ttcctgttcc acgcagcagc tggcggcgtt ggcttgattg cctgccagtg
     4741 ggcaaaagcc ctgggcgcga aacttatcgg caccgtagga accgcgcaaa aagcgcagag
     4801 cgcgctaaaa gcgggcgcgt ggcaggttat taactatcgt gaagaggatc tggtcgagcg
     4861 gttaaaagag atcaccggcg gtaagaaagt gcgcgtggtg tacgattccg tgggcagaga
     4921 cacctgggaa cggtcgctgg attgcctgca acgccgcggc ttaatggtca gttttggcaa
     4981 ctcatcaggt gcggttaccg gtgtgaactt aggcattctc aatcaaaaag gctcgttgta
     5041 tgtgacacgc ccttccctgc aaggctatat caccacgcgg gaggaattaa ccgaggccag
     5101 taatgaactg ttctctttaa ttgccagcgg tgtgattaag gtcgatgtcg ccgagcagca
     5161 gaaatatccg ctgaaggatg cgcagcgtgc gcatgagatt ctggaaagcc gggcgacgca
     5221 aggttccagc ctgttgattc cataaaagaa atagggcttc cacctgggaa gccctttctt
     5281 tttatagttc ggctgtatgt agggtacagc acgatgaatc tgttagaggc gcaatagtga
     5341 cagatttgat tatcaattcc tattttgttc taaggataaa accttaggtt gtgatcatcc
     5401 gcacaatccc ttagtaacgc cagcggtcat aacgctgata tttcggcact tttggtgctt
     5461 taatcgcctt aataacccac accaccgcaa tcgccagtag taaccacggc agcaacttaa
     5521 tcatcaatgc cagcataccg ccgaggaaca taatggccgt cgccacaacc agcgcggcga
     5581 taatgcccag caacgaaacg ccggtgacca tcagcatgac aaaaaagcca atcacaaaaa
     5641 gtagttccag catgatgctc tcccaaatat gaaatctctt gctggcatta caagaatcat
     5701 gccaaaaata atctattgat ttaacagcaa aacgccccgc gacggtgcgc agggcgtggt
     5761 gaatttgact actttttggt gaaaagttaa cgcttatccg ccaccagttt gagcgcgtgt
     5821 tccagcacat taatgtctgc acccgcttta tgggcatttt cacttaaata acgccgccac
     5881 tgccgcgcgc caggaatacc ctggaacaag cccaacatat gccgggtaat atggccgaga
     5941 tacgtcccct ggctgagttc acgctcaatg tacggataca tggcgcgcac taccgccacc
     6001 ggatcggcat cggtatccga ggaaccaaag atctcccggt ctactgccgc cagaataccc
     6061 ggattctgat acgcctcgcg cccgaccatc acgccatcca tatgttgcag gtgtgctttg
     6121 gcctcttcca gcgacttgat accaccgtta atcgacattg tcagatgcgg aaagtcacgc
     6181 ttcagttgat acacacgcgg ataatcgagc ggcgggattt cacggttttc tttcgggctt
     6241 aaccccgaaa gccaggcttt acgtgcgtgg atgataaaca tctcacactc gcctttgccg
     6301 gaaacagtgt tgatgaaatc gcagagaaat tcatagctgt cctggtcatc gatgccaata
     6361 cgcgttttca ccgtcactgg aatcgacacc acatcgcgca tcgctttcac acagtcggca
     6421 accagctgcg cattacccat cagacacgca ccaaacatgc cgttctgcac ccggtcagac
     6481 gggcagccga cattcaggtt gatctcatca tatccgcgcg cttctgccag ctttgcacac
     6541 tgcgccagcg ccgccggatc gctaccgccc agttgcaacg ctaccggatg ttcttcttca
     6601 ctgtacgcca gataatcacc tttaccgtga ataatcgccc ctgtggtcac catttcggta
     6661 tacagcaacg tattgcggga aagcagacgc aagaaatagc ggcaatgtct gtccgtccag
     6721 tcgagcattg gtgcaacgct aaaccgacca ctccagtgaa cgtcagtttt ttcaggcatt
     6781 gcgctggttt ggttgatttt ttgcatttca gaattaccgt gcattttcaa atgtagagat
     6841 tattttatcg atatatcatg gggttatgtt attcagcatc actattcagg aggctcaata
     6901 gcggggtact ataccataac aacaggaagc gcctgtctca ttgcaaaaga aaattgagat
     6961 cctctcaagg catgaagctc tcacgaagtg atggaaataa tcttattagc cgttaacttt
     7021 gttaaggcca atgataaaca atccaggttc gacgataaat aaaaaatcac acattaaact
     7081 ctggtgatat atctccctgc taatagcatt gatagagaaa aaagaaccca ataaatattg
     7141 ggtcctttta tataatgcct tccatactat cgaagaactt cacatattat ttctccgatt
     7201 taaccccgaa caaatcataa attaatttag aagtgtctgt aagtatttta atctcttcct
     7261 ttgaggttgg gtcaaacgac ttcgcgaagc taattaattg tggtgcagca tctctcattt
     7321 tgcttaaaat atcaggatcg agcgttcctt cattcaccag atgtgacatc ttatccagat
     7381 agccatcaaa attcatttct cccccatcag ccagacgctt catctctccc aggtacttct
     7441 tcatatcacg ttgagataat ttttcaaact gagctttcag gtagctctca ttattttcat
     7501 taatatacac tgtttctgat aaatctgcgt aggcactaga atatgaaagt gaaattaaca
     7561 gcgaagcaaa taattttaag ctgttgtcat catgttcctg gcaggcatta acaaacgtta
     7621 aaaaaccaga acctattgct tgaaaatgta tattcgctaa aggttcatta tccttagatt
     7681 cctcataaaa tagctgaacc gtggagggaa ggccattctt agtttctaca tcaaaagtac
     7741 attgttcaat gggagtcgca tctttattat ccccaggatt acatatacta ttaatcgcga
     7801 aatgaaaaat agcccggtca acaactgatg caataaagtc acgatcgttt aaatcctcat
     7861 cagtgcaatc gtcaatatac ttattgaccc acattttgag atcatttatt tgctcaatta
     7921 aaaacacatt tttttacgtg catgccataa attatgcgtg attcgttgaa taaacaaatc
     7981 tatttttgca ataattatat cccgatgacg cagcgctgca taatcaatag atttaccata
     8041 tatattattt aatttacctt tgattgaacc tctattatct aatttatctt tgattgtatc
     8101 ttcattatcg aattgatttg aattgagtgt tttattaaag tatccagttg caccgggtat
     8161 tatcttcaac atgattcatc tacctttttt gtcgtaccgt gtcctcctta taattctttt
     8221 gccatctggt cactatcaaa aatcgctcat aatttctgaa ctacaggtat gcgtttaaac
     8281 ccagttaaat aaatagcgag ttaacttgaa gcaatttatt taaaaatgaa ttattaatta
     8341 ttatcgtgtt gataatggta cgacaaggca acacaacacc ttacctacaa aacagaaaat
     8401 caacattacg gcaacaataa gggttctcgg tgtttttcct ggaacatggt aaagtaagga
     8461 cattcttaac ccccactttg aggtgcccga tggaaaagac cacaacgcag gagttattag
     8521 cgcaggctga aaaaatctgc gcgcagcgta atgtgcgcct gaccccacag cgcctggaag
     8581 tgttgcgcct gatgagtctg caggatggcg ctatcagcgc ttatgatctg cttgatttac
     8641 tgcgcgaagc tgaaccgcaa gccaagccgc caacggttta tcgcgcgctg gattttctgc
     8701 ttgagcaagg ttttgtgcat aaggtggaat ccaccaacag ttatgtgctc tgtcatctgt
     8761 tcgaccagcc cacgcatacg tcagccatgt ttatatgcga tcgctgcggc gcggtgaaag
     8821 aagagtgtgc agaaggcgtg gaagacatta tgcatacgct ggcggcaaaa atggggtttg
     8881 ccctgcggca taatgtgatt gaagcacatg ggctctgtgc ggcatgcgtg gaagtggaag
     8941 cgtgtcgtca tcctgaacag tgccagcatg atcactctgt gcaggtgaaa aagaaaccgc
     9001 gttaagaggg cgtacatcct tgtacacgtc gggcaggagg gattaattac cagcgatatt
     9061 cattgcgggt ttcccaatcc acgacctctt tttctgcctg atctttctga taaccataac
     9121 gttcctggat tttaccgacc agttgatcac gtttaccttc aatgatcgtc atatcatcat
     9181 cggtcagttt gccccattgc tctttcactt tacctttaaa ctgtttccag ttaccgccgg
     9241 cttcatcttt attcataatc aagacctcat cgttaggttg tgaatgagag tacgttcact
     9301 tttcttctga acgtgagatt aagtgtagtc agcaatttgg cttaggataa tttattcaga
     9361 atttttaacc gtcacgttgc ggcaaaccag gtaccgttgc gccagtgacg ccgccagata
     9421 gccgccagag aaagcccgcg caacgccaga aagacggtta atgccagcca caaaccatga
     9481 ttacccagcc acggcagcgt aaggagcgtc agcgcaaaac ctgcggcggc caccgccata
     9541 ctgttacgca tttcggcggc acgcgttgcg cctataaaca tgccgtccag cagataacac
     9601 cagacgccaa ccaacggcaa aatcacctgc cagataagat agcggtcagc cagctgctga
     9661 atctgggtta acgacgtcag caacgcaatg atgtgttccc ctgccagcaa ataaaccacc
     9721 gaaaacagta acgctacaat ccccgactgg cggcacgctg cccgccatac atccagcaac
     9781 tggctaccgt cgcgcgcacc gtatgcctga ccggagtgcg cttcaaccgc gtaggcaaaa
     9841 ccatcaagcg cataggcggt aaaggtgagt agcgtcatca gaaccgcgtt aacagcgaca
     9901 atgtcactcc ccagtcgcgc accaagtacg gtgatcgcgc cgaaacagag ttgcaacaac
     9961 agcgaacgca gcatgatatc gcggttaagc gccagcaagc gacggaagtt tcctcgccag
    10021 gcagttttca gcatttcgcc ggaaattccg cgtagtttga ggattttacg caccattagc
    10081 agaccaatca gcaatgttgc atattccgca ataaccgtcg ccagcgccgc gccctgcacg
    10141 ttcatatgca gccccatcac cagccagaca tccagcacaa tgttgaggat attgccgacc
    10201 actaacaaaa ttactggcgc acgggcatat tgcacgccga gtaaccaacc gagtaatacc
    10261 agattcgcca gcgacgccgg agcgcttaac cagcggattt caagaaagcg ccgcgcctgt
    10321 tccaggaccg cttcactacc gccaacaata tgcagcgcca gatcgataat cggtgtacgc
    10381 agcagcgcaa ttaacgcccc agcccccaac gccaacagca acggttgcac cagcgcacgg
    10441 gctaatgcct gaggattttt ggcaccataa gcctgcgcag tcagcccggt ggtgctcatg
    10501 cgtaaaaaca gcaacagcat aaagagaaag ctggtcgccg ttgcaccaac cgccacgccg
    10561 cccaaataaa ccgggctatc aagatgacca attaccgccg tatcgaccag tcccagcaac
    10621 ggaacggtga tattggagaa aatcatgggt aaggcgagat gccagagtgc tttatcagat
    10681 gaagtgagga atgccatgca gacaagcctg atgaagagag atgaaaaaca aaccgcgacg
    10741 cctggcggca tcgcggtctc agagatatgt tacagccagt cgccgttgcg aataacccca
    10801 accgccagcc cttcaatggt gaagctctgc tgacgaaggt caacgacaat tggtttaaac
    10861 tcgctatttt ctggcaacag ttcgacttta ttgccctgtt ttttcaggcg cttaacggta
    10921 acttcgtcat caatacgtgc gacaacgacc tgaccgttac gtacatcctg agttttatgc
    10981 actgccagca agtcaccatc cataatgccg atatctttca tcgacatccc gctgacgcgc
    11041 agcaggaaat cagcattcgg cttgaacaag gaaggatcga cctgataatg accttcaata
    11101 tgctgttgcg ccagaagcgg ttcaccggca gccacacgac ctaccagcgg caacccttct
    11161 tcctcttcct gcaacagacg aatcccgcgt gatgcgccgg aaacaatttc aataacgcct
    11221 ttgcgtgcca gcgccttcag atgttcttca gccgcgtttg gggaacggaa ccccaaacgc
    11281 tgcgcgattt ccgcacgcgt cggcggcata cctgtctggc tgatgtgatc acggatgaga
    11341 tcaaacacct cttgttgcct ggccgttaac gctttcattc cgccccctgg gtgtatatac
    11401 agttatgctg tgagtatata cagcaaaagg cgattttgga accataaact gcacaataaa
    11461 ccagagattt atcgaaatct ggaagggtta tccaaaatgc gaccataaca gaatgcacca
    11521 ggtaatcacg gcgacgataa tggcaatcag caccgcggcg gatcccatat ctttagcgcg
    11581 tccggaaagc tcatggtatt cagagccaat tcggtcaacc actgcttcga tggcgctatt
    11641 gagaatttcc acaatcatca ccagcatcac ggagctgata agcagcacgc gggtaattgc
    11701 gtccacatcc agccagcagg cgatgaccac cgccaacaat accgctacgc cttcctgacg
    11761 gaatgccgct tcgttgatcc atgcagcgcg taaacctttc caggaatagc cagcagcttt
    11821 gataattcgg gtgaatccag tggtattatt ggccattgaa agaacctttt tacattatga
    11881 gcgtcaatat cagtgtaccg ttaaatccga tgattcgggt ttgcgtaacg ccgcgcgaaa
    11941 catgagcgga taccacagaa tttcccatga ctttctgcta tccttgccgc gcatttgcat
    12001 tattaaccag aggctttaca tcgtttatgt ccggctggcc acgaatttac tacaaattac
    12061 tgaatttacc attaagcatc ctggtaaaaa gcaagtctat tccggcagat cctgccccgg
    12121 aactggggct ggatacttct cgtccaatta tgtacgtttt accgtacaac tcgaaagcag
    12181 atttgctgac gttacgcgcc cagtgtctgg ctcatgactt gcctgacccg ttagagccgc
    12241 tggaaatcga cggcacgcta ctgccgcgct atgtgttcat tcacggcggg ccgcgtgtat
    12301 tcacctatta cacgccgaaa gaagagtcta ttaagctgtt ccacgactat ctcgatttgc
    12361 accgtagcaa cccaaatctg gatgtgcaga tggtgccagt atcggtgatg tttggtcgcg
    12421 cgccggggcg tgaaaaaggc gaagtaaacc cgccgctgcg tatgcttaac ggcgtacaga
    12481 aatttttcgc cgtactgtgg ctcggtcgcg acagttttgt gcgtttctcg ccgtcagttt
    12541 cgctgcgccg tatggcggat gaacacggca cggataaaac tatcgcacag aaactggcgc
    12601 gcgtggcgcg tatgcacttt gcccgtcaac gtctggctgc tgtgggccca cgtctgcctg
    12661 ctcgtcagga tctgtttaat aagctgctcg cctcccgcgc cattgccaaa gcggtagaag
    12721 atgaagcgcg cagcaaaaaa atctcccatg aaaaagcgca gcagaacgcg attgcgctga
    12781 tggaagagat tgcggcgaat ttctcttacg agatgatccg cctgactgac cgcattctgg
    12841 gcttcacctg gaaccgactt tatcagggca tcaacgtcca taacgccgag cgcgttcgcc
    12901 agctggccca cgacggtcat gagctggtgt atgtgccttg ccaccgcagt cacatggact
    12961 acctgctact ttcttacgtg ctgtaccacc aggggctggt gccgccgcat atcgccgccg
    13021 ggatcaacct gaacttctgg ccggccgggc cgattttccg ccgtctgggg gcgttcttta
    13081 ttcgccgtac ttttaaaggc aataaacttt attccaccgt tttccgtgag tatctcggcg
    13141 aactgttcag ccgtggttat tccgtcgagt acttcgtgga aggcggtcgt tcccgcacgg
    13201 ggcgtttgct ggatccgaaa accggtacgc tgtcgatgac cattcaggcg atgctgcgtg
    13261 gcggcacgcg tccgattacg ctgattccga tctatatcgg ttatgagcac gtcatggaag
    13321 tgggtactta cgccaaagaa ctgcgcggcg cgacgaaaga gaaagagagc ctgccgcaga
    13381 tgctgcgcgg tttaagcaag ctgcgtaatc tcggtcaggg ttacgtcaac ttcggtgaac
    13441 caatgccgtt gatgacctac cttaaccagc acgtaccaga ctggcgtgaa tctatcgatc
    13501 ccatcgaagc ggtgcgtccg gcctggttaa cgccgacggt caataatatt gctgccgatc
    13561 tgatggtacg cattaacaac gcaggcgcgg caaacgccat gaacctgtgc tgtactgcgc
    13621 tactggcatc acgtcagcgc tcactcaccc gcgagcagtt aaccgagcaa ctcaactgct
    13681 acctggatct gatgcgcaac gtaccttact ccacggactc taccgttcct tcagccagcg
    13741 ccagcgagct tatcgatcac gcgctgcaaa tgaacaagtt tgaagtcgag aaagacacta
    13801 tcggcgacat catcattctg ccacgcgagc aagcggtgct gatgacctac tatcgcaaca
    13861 acattgcgca tatgttggtg ctgccttcgc tgatggcggc aattgtcacc cagcatcgcc
    13921 acatctcccg cgacgtattg atggagcacg tcaatgtgct ttacccaatg ctgaaagcgg
    13981 agctgttcct gcgctgggat cgcgacgagt tgccggacgt tattgatgcg ctggcaaatg
    14041 agatgcaacg tcaggggctg attaccctgc aagatgatga gttgcatatc aacccggcgc
    14101 attctcgcac cctacagctg ctggccgcag gcgcgcgcga aacgctgcaa cgttatgcca
    14161 tcaccttctg gttgttgagt gccaacccgt cgatcaaccg cggtacgctg gagaaagaga
    14221 gccgcaccgt cgcgcaacgt ctctccgtgc tgcacggcat caacgcgccg gagttcttcg
    14281 acaaggcggt gttcagttct ctggtgctga cgctgcgtga tgaagggtat atcagcgata
    14341 gcggcgacgc cgaaccggca gaaacgatga aggtttatca gttgctggcg gagttgatta
    14401 catcagacgt gcgtttgacg attgagagtg cgacgcaggg cgaagggtaa tcagagagaa
    14461 ttgccggatg cggcgaaaac gccttatccg gccttccata atctgcaaat tcaataaatt
    14521 gcgatgtgtt gtgtaggcct gagaagcgcc gcgcatcagg caacccagaa gaaagccgga
    14581 tgatcatccg gcttttttac atcatcagaa atgccagtaa ctcattgcca gccctaaaaa
    14641 tagtaccaga ccaacatagt tattattcat aaatgcttta aagcaggctt cacgctcgcg
    14701 gttggcaatc agtttttgtt gataaacaaa cagcgcgcca gccaccacaa ttgaccagta
    14761 atatccccag cctaagccat ttaactcacc gatgatcgcc atcagtgcca gtacgccaat
    14821 ctgcaaaata ccaataatca atttatcgta ttggccgaac aggattgccg tggatttaat
    14881 gccaatcttc acatcatcat cgcggtcaac catcgcatac tgcgtgtcgt aagccaccgc
    14941 ccagagaata ttggcgagga acattaacca gcaactcaat ggcaccgact cactcacagc
    15001 ggcaaaagcc attggaatcg accagccaaa cgccgcgccc agcaccactt gcgggagatg
    15061 ggtataccgc ttcataaacg ggtacaccca cgccaacgcc agagcggcaa tcgacaacag
    15121 aatggtcatc gtattcagcg tcagtaccag taaaaacgaa atcagtacca gcactacaaa
    15181 cagtgcgcgc gcctctttct ctgttaccgc gccgctggga agtggtcggt tcgccgtgcg
    15241 cttaacatga ccatcaaact ttcggtcagc ataatcattc accacacatc cagcagcgcg
    15301 catcagccag acacccgcga caaataccgc caggatccag agctgaggaa cgcccggtgt
    15361 cgccacccac aacgcccata atgttggcca gagcagcagt aacgcgccaa ttggcttatc
    15421 cgtacgcatt aagcgatgaa acgccagcag cttattctgc gtcagactcc actccatatt
    15481 tttttcctct tagtacaacg gtgacgccgg taaaaacagt tctgttagca acagcggttt
    15541 accgcttaat cgcaggcggg aacgtcgccc ccacagtccg gcatcacggc ctatctcaat
    15601 aaagtcccgg gttaatgtcg atgatgtgaa cagatagcgt cctaacggcg ttttacccaa
    15661 tttttgtaac gccagctccg gcccgcttaa cgttgacaca ggaacgacgg tacgaccggc
    15721 aagccacggt tcaccatcgg cacataacaa aatttcacgt aaccagtaac gagactcttt
    15781 cggcagcagc ggcagttctt cggggatttc attctgctcg acaaaccctt cgcggatcat
    15841 cgtcacgctt accgtttttc cctgctgttc aaaacgtttt gtcatggaat cctccagcaa
    15901 cagccagtcg agcagttgcg gatccagggc agggatctct ttaaaatagc gcagcgcacg
    15961 cagttgcgtt aacgcggggt gtgacatgcc gaactctccg ttacataacg taaaggcatt
    16021 gtatcgcaga aaagtgttta cgggagatgt aaagcaaatt aagtgatcta atgcgcaaca
    16081 aaaacgcaac aatcgcatga agtggtaaac cgctgttgaa ccttacgctt tgccgatgcc
    16141 cgtgctggcg agggcaaccg taggggcatc accgcccatg atgccaggaa cggtgatgtt
    16201 ttcctttact taaataccta tattctttat acgagtagct cgccaactcg cagtaaaaat
    16261 tgggaaaagc gcatatttac catgagattt ggagctaaaa agtgtttcct gagtaagatt
    16321 aataggcaac atcttatgtc tggatttata gatacacgtg gaattccaat tggtatcttt
    16381 tgtaggattc aacatatcat gtaagcgctg ggagcttacc accataactg gcccatcatc
    16441 ggcagaggag aacaggataa agatattatc ctttcgtgtt aattcattcg tgttaaaggt
    16501 tcttaaatta tcttcaattt ctataaatag aggtgaaaca tcatcactac gaatccactc
    16561 tttataaaga taccaggcat ttgctttgat catatcatcc gttgatcgta aggcctgatc
    16621 gataagttgg ataaaaaaat tgttattttt atcaatcagc cctttattca tattaaacct
    16681 gttaataaaa tggatcatct gttgggtatc gaaatccacc ttgctgaatc gatgttgacg
    16741 cataaaatca tcgaacttgt tatcttcacc agaatggata taactgcgta taattggatc
    16801 ttt
//
LOCUS       DAZFXT010000103        16708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.103, whole genome shotgun sequence.
ACCESSION   DAZFXT010000103 DAZFXT010000000
VERSION     DAZFXT010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.103"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            87..314
                     /locus_tag="ACHKW0_003586"
     CDS             87..314
                     /locus_tag="ACHKW0_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020232163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476353.1"
                     /translation="MACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILF
                     KMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(391..633)
                     /gene="yfdY"
                     /locus_tag="ACHKW0_003587"
     CDS             complement(391..633)
                     /gene="yfdY"
                     /locus_tag="ACHKW0_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HFO7476354.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            986..1906
                     /gene="lpxP"
                     /locus_tag="ACHKW0_003588"
     CDS             986..1906
                     /gene="lpxP"
                     /locus_tag="ACHKW0_003588"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008951 - palmitoleoyl
                     [acyl-carrier-protein]-dependent acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009409 - response to cold [Evidence IEA];
                     GO_process: GO:0036104 - Kdo2-lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HFO7476355.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2262..2333
                     /gene="ypdK"
                     /locus_tag="ACHKW0_003589"
     CDS             2262..2333
                     /gene="ypdK"
                     /locus_tag="ACHKW0_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HFO7476356.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2398..3636)
                     /gene="alaC"
                     /locus_tag="ACHKW0_003590"
     CDS             complement(2398..3636)
                     /gene="alaC"
                     /locus_tag="ACHKW0_003590"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HFO7476357.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DDWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            4013..5710
                     /gene="ypdA"
                     /locus_tag="ACHKW0_003591"
     CDS             4013..5710
                     /gene="ypdA"
                     /locus_tag="ACHKW0_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HFO7476358.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5725..6459
                     /gene="ypdB"
                     /locus_tag="ACHKW0_003592"
     CDS             5725..6459
                     /gene="ypdB"
                     /locus_tag="ACHKW0_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HFO7476359.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6472..7329
                     /gene="ypdC"
                     /locus_tag="ACHKW0_003593"
     CDS             6472..7329
                     /gene="ypdC"
                     /locus_tag="ACHKW0_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7476360.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7332..9827)
                     /gene="ptsP"
                     /locus_tag="ACHKW0_003594"
     CDS             complement(7332..9827)
                     /gene="ptsP"
                     /locus_tag="ACHKW0_003594"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HFO7476361.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASVQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDRDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9852..10889)
                     /gene="ypdE"
                     /locus_tag="ACHKW0_003595"
     CDS             complement(9852..10889)
                     /gene="ypdE"
                     /locus_tag="ACHKW0_003595"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7476362.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10889..11974)
                     /gene="ypdF"
                     /locus_tag="ACHKW0_003596"
     CDS             complement(10889..11974)
                     /gene="ypdF"
                     /locus_tag="ACHKW0_003596"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7476363.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11989..13236)
                     /gene="fryC"
                     /locus_tag="ACHKW0_003597"
     CDS             complement(11989..13236)
                     /gene="fryC"
                     /locus_tag="ACHKW0_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HFO7476364.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13258..13584)
                     /gene="fryB"
                     /locus_tag="ACHKW0_003598"
     CDS             complement(13258..13584)
                     /gene="fryB"
                     /locus_tag="ACHKW0_003598"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0022877 -
                     protein-N(PI)-phosphohistidine-fructose phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_function: GO:0090563 - protein-phosphocysteine-sugar
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0090582 -
                     protein-phosphocysteine-D-fructose-phosphotransferase
                     system transporter activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HFO7476365.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(13803..14768)
                     /gene="glk"
                     /locus_tag="ACHKW0_003599"
     CDS             complement(13803..14768)
                     /gene="glk"
                     /locus_tag="ACHKW0_003599"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - D-glucose binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HFO7476366.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14972..16228
                     /gene="yfeO"
                     /locus_tag="ACHKW0_003600"
     CDS             14972..16228
                     /gene="yfeO"
                     /locus_tag="ACHKW0_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HFO7476367.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPFWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16343..16669
                     /gene="ypeC"
                     /locus_tag="ACHKW0_003601"
     CDS             16343..16669
                     /gene="ypeC"
                     /locus_tag="ACHKW0_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein YpeC"
                     /protein_id="HFO7476368.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
BASE COUNT         4032 a         4460 c         4194 g         4022 t
ORIGIN      
        1 cgtaggttct cagtgaacca ttttcaggat aaatttctat gaaagtaaac ttaatacttt
       61 tcagcttatt tttattggtc tctattatgg catgcaatgt ttttgcattt tccatttctg
      121 gtggtgtaag tgagaggagc tataaagaga ctgaaaaaac atcagcgatg acgaccacac
      181 actctacaaa acttcagcca tcacaggcga ttttgtttaa gatgagagaa gatgcgccac
      241 cattaaacct cacagaagaa atcacgccta cttatccgac aaaggcgaat tatcttattc
      301 atcctgtgcg atagcgtctg tcacatggca ggtgaacaat catgtctgat gctgatgtga
      361 acacctgccc aaatgagcca tcctgaccca ctatcgcctg ccatacccca ccataaagcg
      421 aatcatcaag ccacaagccg gggcaagaaa cataaatatc cccgtcacta gttcattacc
      481 gccagttaac cacatggtga agtaataaat gagtgcagcg aggatcacca tgccgaagaa
      541 agaagagacc gccgatacca cgttcagtac ctgaccgatg tagccgctta cgcagacaat
      601 acacagggcg agaaacatcc ataaataaat catagctcaa cctcctgccg cgctaaaaaa
      661 cctgtaactt cagtcacttt aatagtaaga caatccttaa cctctggcaa tttttgctaa
      721 gcatcaatga aaacagcatg ttaaatgcaa gactgttgtg tacggaaaaa tatttacttt
      781 gcacgattaa taatcagtag ctgaaagcag tcagcgagtg ataaagtaca gataccgcat
      841 tcatgctcct cgtggttatg tcctgactag tctttacact ctttacagga accattgtcg
      901 tacatgatgg cccaaccaat tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa
      961 caattcgtat ctacagaagg taactatgtt tccacaatgc aaattttccc gcgagtttct
     1021 acatcctcgc tactggctca catggtttgg gcttggtgta ctctggcttt gggtacagct
     1081 tccttatcct gttctctgct ttctcggcac gcgtattggc gcaatggcgc gaccattcct
     1141 gaaacgtcgt gaatctatcg cccgtaaaaa cctggaactt tgtttcccgc agcattctgc
     1201 ggaagaacgc gagaagatga ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga
     1261 aaccggcatg gcatggttct ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg
     1321 gttggataac cttaaacgcg cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca
     1381 ttttatgtcg ctggaactgg gcggccgcgt gatgggactg tgccaaccaa tgatggctac
     1441 ctatcgtcca cataataatc agctgatgga atgggtgcag acccgtgggc gcatgcgctc
     1501 taacaaagcg atgatcggca gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg
     1561 tgaagcggta tggtttgctc ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc
     1621 gttctttgcg gtggaaaatg tcgccacaac caatggcacc tatgttctct cccgtctctc
     1681 tggcgcagcc atgttgaccg taacgatggt aagaaaagcg gattacagcg gatatcgttt
     1741 gttcatcacc ccagagatgg aaggctaccc gacagatgaa aatcaagccg ctgcctatat
     1801 gaacaagatt atcgagaaag agatcatgcg cgcaccggag cagtacctct ggatccaccg
     1861 tcgctttaaa acgcgcccgg tgggagaatc gtcgttgtac atttaacaaa ctgctcttac
     1921 tctttccctc ctcaaatcgg ccataataac cttatggttt ttatggcttt tttcttaagg
     1981 ttttttcctt cgctgaatat ctaattatta ccgtcagaaa ttaaactgtc gccgatatga
     2041 gacacttgta agttactaaa agtaaataag aaaatgtctc ttgtctcgtt tcagttttag
     2101 gcgtacatta gcgccgtctc gagcagttta gctcagaatt ctggggtgat gcccacggta
     2161 aaatacaggg cttttaaggt cgctttgccc ggtgtcaact cactgtatca ggtgtaatga
     2221 agtcattcag gcgtaacagt aattacgcgg agagatgtaa agtgaaatat ttctttatgg
     2281 gcatttcttt tatggtcatc gtttgggccg gtacttttgc cctgatgatc taaaagcaaa
     2341 ctgtgcaatg aaaaacagga gccatcaggc tcctgtttgc atttctgacg acggtgttta
     2401 ttccgcgttt tcgtgaatat gtttgctgct ggcgggtagt aaaccgtcgg cacggaacat
     2461 cgctttaatc ccacgaatcg cctgacgaat acggtcgcgg ttttcgatca gtgcaaagcg
     2521 aacatgcgta tcaccatagt cgccaaagcc aatccccggc gagacacaaa ccttcgcttc
     2581 gttaaggagc ttcttggcaa attccagcga tcccatggcc gcatatggtt ccgggatttt
     2641 cgcccagaca tacatcgaag ccttcggcat ttcaaccatc cagcccgctt catgcagccc
     2701 tttaaccaat acatcacggc ggcgtttgta ctgttcggca atgtcgcgca cgcactgttg
     2761 atcgccctcc agcgccgcaa tcgccgccac ctgcaacggc gtaaaggtgc cgtaatcgtg
     2821 atagctttta atacgagcca gggcgctgac cagcgttttg ttgccaacca taaagccgat
     2881 acgccagccc gccatgttgt agcttttcga cagcgtaaag aactcgactg ccacatcgcg
     2941 tgcacccggt acctgcatga ttgacggcgc tttccagtca tcgtagacga tatcggcata
     3001 ggccaggtcg tggaccacca gcacatcgta gcgtttcgcc agcgccacaa ccttttcgaa
     3061 gaactccagc tccacgcatt gcgcggttgg attcgacggg aagccgagga tcatcatctt
     3121 cggtttcgga taactttcac gaatggcgcg ctccagttcg ttgaagaaat cgacaccttc
     3181 caccagcggc actgagcgta cctgcgcccc ggcaatcacc gcgccataaa tatggatcgg
     3241 gtaacttgga ttcggcacca gcaccgtgtc accatgatcc agcgtcgcca gcatcagatg
     3301 cgccaggccc tctttcgaac caatagtgac gatggcttct gattccgggt cgatttcaac
     3361 gtcgtagcga tcctgatacc agcgggaaat ggcgcgacgt aaccgcggaa tgccgcgtga
     3421 agtggagtaa ccatgcgtgt ccgggcgctg ggccacagta cataattttt cgacgatatg
     3481 cggcggagtc gcaccgtccg ggttacccat gctgaaatcg ataatatctt cgccgcgccg
     3541 acgcgcagcc attttcagtt cagcggtgat gttaaaaacg tagggcggga gacgatcaat
     3601 gcgcgtaaag cgacgttcag ggcgagtgtc agccataatt tcctcagatt aacgttagcg
     3661 cccggaccgt ccgagcgacg ctgccacaat ggtggctgtt ttgaaaatag cctgattaat
     3721 ttctgtctgt ccagcgtttt agaacatttt tatcaatggc tttaaatgag gaactccact
     3781 tcaaaaacag gaaatttttc tacgctaaca agaaaattac caccaatttt attaacatta
     3841 taatttcaat aaatttacca gccagaagtg cattatcaag agatacattg ctaatacacc
     3901 atttatcttt caatcccctt ttaaatttat ggtttttcct caaaaacggc ctgctggtca
     3961 tcagccttca ggttacctat catagaggtt taatccttat tcagagtcac ccgtgcacga
     4021 aatattcaac atgctgctgg cggtcttcga tcgggcagcg ttaatgctta tctgcctgtt
     4081 ctttctcatc cgtatccgcc tgtttcgcga actgttgcac aagtcggcgc actccccaaa
     4141 agaattgctc gccgttaccg ccattttctc gctgttcgcc ctgttcagca cctggtccgg
     4201 cgttcccgta gaaggctcgc tggtgaacgt acgtattatc gcggtgatgt ccggcgggat
     4261 tctgtttggc ccgtgggtag gcatcattac cggcgtgatt gcgggtattc accggtattt
     4321 aattgatatc ggcggcgtga cggcgatccc ctgctttatc accagcattc tggcgggttg
     4381 tatatcaggc tggataaacc tgaaaatccc caaagcacag cgctggcgcg tcggtattct
     4441 cggcggcatg ttgtgtgaga cgttgaccat gattctggta attgtctggg caccaactac
     4501 cgcgttgggg atcgatatcg tctctaaaat cggcattcca atgatcctcg gtagcgtctg
     4561 tatcggcttt attgtgcttc tggtgcaaag cgttgagggc gaaaaagagg ccagcgccgc
     4621 gcggcaggcc aagctggcgc tggatatcgc caacaaaacg ctacctctgt ttcgccatgt
     4681 caatagcgag tcattacgca aagtttgtga aattattcgc gatgacattc acgccgatgc
     4741 ggtggcgatt accaataccg atcatgtgct ggcctatgtt ggcgtgggtg aacataacta
     4801 tcagaatggc gatgacttca ttagcccgac tacccgtcag gcgatgaatt acggaaaaat
     4861 catcattaaa aacaatgatg aagcccaccg cacaccagag attcattcca tgctggtgat
     4921 cccattgtgg gagaaaggcg ttgtgaccgg aacgctgaaa atttactact gccacgcgca
     4981 tcagatcacc tcgtcattac aggaaatggc ggtcggtctg tcgcaaatca tctccaccca
     5041 actggaggtt tcacgcgccg agcagctacg tgaaatggca aataaggcag agcttcgcgc
     5101 cctgcaaagc aaaattaatc cccatttcct gtttaacgct ttgaacgcta tttcatcatc
     5161 aatccgtctg aatccggata ctgctcgcca gctgatcttt aatctgtcgc gttatctgcg
     5221 ctataacatt gaattaaaag acgatgagca aatcgatatc aaaaaagagc tgtatcaaat
     5281 taaagactat attgccattg agcaggcccg ctttggcgac aagctgacgg ttatctatga
     5341 tattgatgaa gaggtgaatt gctgcattcc cagcctgctg atccagccgt tggtggagaa
     5401 tgccattgtc cacggtattc agccttgcaa aggtaaaggc gttgtcacca tcagcgttgc
     5461 agagtgcggc aatcgggtac gcattgcggt gcgggatacc gggcacggca tcgatccaaa
     5521 ggtgattgag cgggtcgaag cgaatgaaat gccgggcaat aaaattggcc tgctgaatgt
     5581 tcatcatcgc gtgaagttat tgtatggcga ggggctgcat atccgccgcc tggaaccagg
     5641 aacggaaatt gcgttttaca ttcctaacca acgcacccca gtcgcctcac aggctacgtt
     5701 attgctttga gccggagtga tattgtgaaa gtcatcattg ttgaagacga attcctggca
     5761 caacaggaac tgagctggct aattaaagag cacagccaga tggagattgt cggcaccttt
     5821 gacgacggtc tggacgtgtt gaagtttttg cagcataacc gcgtcgacgc catttttctg
     5881 gatatcaata ttccgtcgct ggatggcgtg ttgctggcgc aaaacatcag ccagttcgcc
     5941 cataaaccgt ttattgtgtt catcaccgcg tggaaagaac atgcggtaga agcgtttgaa
     6001 ctggaggcgt ttgactacat tctcaaaccg tatcaggagt cacgtattac cgggatgctg
     6061 caaaaactgg aagcggcctg gcaacaacag cagaccagca gtacgcctgc cgcgacggta
     6121 acgcgtgaga atgacaccat taatctggtg aaagatgagc gaataatcgt cacgccaatt
     6181 aacgatatct attacgccga agcgcacgag aaaatgacct ttgtctatac gcggcgtgaa
     6241 tcctacgtaa tgccgatgaa cattaccgaa ttttgcagca aactgccgcc gtcgcatttt
     6301 ttccgctgcc atcgctcatt ttgtgtcaat ctgaacaaaa tacgcgaaat cgaaccgtgg
     6361 tttaataaca cctacattct gcgactgaaa gatctggatt ttgaagtgcc ggtcagccgc
     6421 agcaaagtga aagaatttcg ccagttaatg catctttaat cgggaacttt catgaaagca
     6481 ccaggcttgc ccgccgatca acaatttttc gccgatctgt tcagcggcct ggtgcttaac
     6541 ccgcaactac tggggcgcgt ctggtttgcc agccagcctg cctcgttacc ggtgggcagt
     6601 ttatgtattg attttccccg tctggatatc gtgctgcgcg gcgaatacgg caacctgctg
     6661 gaagcaaagc agcagcgaat ggtggaagga gaaatgctgt ttattccggc acgcgcggct
     6721 aatttaccga tcaacaacaa accggtgatg ctgttaagcc tggtgttcgc accgacctgg
     6781 cttgggttat cgttttacga tagccgcacc acgtcgttgt tgcatcctgc tcgccagatc
     6841 cagcttccca gcctgcaacg cggtgaaggt gaagcgatgc ttaccgccct cacccatctt
     6901 agccgttcgc cgctggagca gaatatcatt cagccactgg tgttaagttt gctgcatctt
     6961 tgccgtaacg tggtgaatat gccgccgggc aattcgcagc cgcgcggcga ttttctctat
     7021 cacagcattt gtaactgggt tcaggataat tatgcccagc cgctcacccg cgagagcgtg
     7081 gcgcagtttt ttaatatcac gcccaatcat ctgtcaaaac tgtttgctca gcatggaacg
     7141 atgcgtttta tcgagtatgt gcgttgggtg cgaatggcga aggcgaggat gattttgcag
     7201 aaatatcatc tgtcaattca tgaagtggca cagcgttgcg gttttccgga tagcgactat
     7261 ttttgtcgcg ttttccggcg tcagtttggt ctgacgccgg gagagtacag cgcccgtttt
     7321 cagggctaac gtcagaaggt taattctgtt tccagcagcg tcaggatact ttgtgcatct
     7381 tgcgcggcaa acaacgactg gcggaagttt ttattcacca gtttacgcgc cagctgcgag
     7441 aagactttca catgattcat cccttcgtta gcacccagcg tcagcatgat caccagttcg
     7501 acttcgccca tttctgactg ccagtcaatc ggtttcgcca gccgggcaat gctgatactg
     7561 gaatgacgga tccactgaga tttggtgtgc gggatcgcta cgccaaaacc aacgccggtg
     7621 gtaacaatct cttcccgctg ccagacatct tcttccagct caaacggatg ttcagtgcgc
     7681 ccgttaacgc cgaggttgcc gcacaggaac tggatcgcct gctctttatt gctaaaatcc
     7741 cgatcaacaa agatattctc cagcgccagc agtgggcgaa cgtcttcttc cggcgtaaag
     7801 gcggtgagta acgcttcaat ttcctgcgca ctgcggcatt cacatgcctg acgcgccagt
     7861 tcccgacacg cctcgctatc cagttgacga agctggcttt tcaccgccgg aatacgcggg
     7921 ctactcatac tcagctcatc caggcccagc ccaagcagta gcggcagata acggctttca
     7981 ccgcccagtt caccgcaaat gcctacccat ttgccccgct gatgcgcagt ggtaacaatc
     8041 tgctgcaaca tgcgcaggaa cgatggcgta atcgggttat acagcggcga tacgcgcggg
     8101 ttattacgat cgaccgcata cagatactgg gtcatatcgt tagaaccgat actgaagaaa
     8161 tcgacctcat cgcagaagtg gtcgatgatg taacacaccg acggcacttc caccatgata
     8221 cccagagtaa tcgtctctgc atgacgcagg ccatcgcgct taagctcaac gatcgctttt
     8281 tggatctcgc ccttcaccca taagatctga tcgaggctgt gaaccatcgg gatcatcaac
     8341 tgggcgttgc caaaactggc agcgcgcaaa atcgcccgta gttgagtgcg gaacaggcca
     8401 gcaaattccg ggtaaatacg taccgcgcga tagccgagga acgggttctc ttcctgggga
     8461 atattcagat aaggaatgct tttatcgcct ccgatgtcca ttgtgcggaa gataatcggc
     8521 ttgtcgcccg ctgccagtag cacctgctgg taggcttcaa attgctcctg ctcgtccggc
     8581 gcgctgtcac gatccatata cagcatttcg gtacggaaca gcccgacgcc ttccgcgccg
     8641 ttggcaaacg cgcctggtgc ttccagagcg gtgccaatat tcgccgcgat atcaatacgc
     8701 ttgttatcac gggaataggc cagctgcgcg gcagcctgcg cctgttgttt ttggcgttta
     8761 tccgccagcg tctgcgcaac ctgataataa ccgctcaccg cgtcattcgg gttaatcgcc
     8821 agcacgccgc actgggcgtc aagcaccgcc ggttgcccgg cataacgggc aatcgcatcc
     8881 agcggcaggc cgctcagtac cgggatcgcc gaggcgcggg ccagaatcag ggtatgcgag
     8941 gtgcgcccgg ttttctccag aatcatgccc gcaagatttt tcaaatcaag gctcaaaaac
     9001 tggcttgggg ttaaatcttc agccaccaga atggtcggtt tttccagcac cagattgttg
     9061 cgcggcttca gttccggcca ggtgatatgc agcaactgtt cgctaatatc gcgaatgtcg
     9121 ctaacacgtt cgcgcagata atcgctggca gaagcagaaa gcttggcgca aacctgctcc
     9181 atattgctga tgatcgccgc ccccagtccc tgatgctgtt ctgtcatcag gcgacggata
     9241 ttgcctgcaa attcatcatc ctgaatcagc gacaaatggg cgctgaggat agttttgctt
     9301 tcgccgtcac gctcacgcaa ttgctggttc agttgctcgg caagcgttgc caggctgtgc
     9361 tccagccggg tggaatcttg cacactggcg gggattgccc gataactgtc gaggctgtcg
     9421 ctttgtaaca gggtcagcgt acccacgccg acgccgcttg ccagcacatt gccgtacagt
     9481 aaatccgggt taaggcggct taatgaacgc ggcagcggat gcgccgtcag ttctgcctgc
     9541 gtaggctgga cgctgtcgct gtcgataaag cgcacctgga tgtactcttc cagcacgcgc
     9601 cgcgcctgct cttcatcgct gccgctaatg ttcaggctgc aactgtcatt aaatagggtg
     9661 ccggtgccaa tcagcgccag cgaacttttc gcatcggcct ttgcgttctg gcgatggtta
     9721 ataaaggtga cttcactttg ccactggctg cactgttctt taagttccca cgccggacga
     9781 gcatgcagac cgttaggcaa aggacagaga aattgaatcg ttaacataac cgctccgggt
     9841 taatcaggag atcatctgaa atccgtcagt tgaacaaccg tctcacgggt aagacgttga
     9901 ataagggcag ataaaagttg ctccatctgc aaaatgtcgc ggcaatcggc aatcgatgcc
     9961 gcgcaatgtc cgtggcgggt ggcaggcccc attaccactg tgggtacgcc agtaccggtt
    10021 aagtgcaccg ctccgccgtc cgtgccgccg ttgctgaaca tatctgcctg caacggtacg
    10081 ccaatttcag ccgccacggt ttcgatccag gcggtaagtt ttggcggcgc aatcagtgac
    10141 ttgtcgctta acaccagcat cgggccgtta ccaatctggc gatggttagc cgcgccataa
    10201 tcaaagtttt tcgcccagca ggcggtatcc agcacaatgg cgacgtccgg cgacaccgcg
    10261 cgggtagcgg tttgcccacc gcgtaatccc acctcttcgc tggaacttgc caccagccac
    10321 acctccgcag gcagttcggc atcgtgcagc tcacgtagta acgtcaccag cagatagcaa
    10381 ccgaggcgat catcaaaggc tttccccatc acccgctggt gagggagaac ctgaaaagtt
    10441 gtatcaaagg tgacgcgatc gcctggacga attcccgcct gcatcacttc gtcatacgag
    10501 cgcgcaccaa tgtccacgcg catggcgctg acgtcattcc cctgccggtc gccgtcaagc
    10561 aggcctggaa ttttgcactc ttcacgggtg gtgatgcgca ccggctgcag ctggcgggca
    10621 gccatgcgca cgttgccaac cggcagcaca tcaattgccc cttcgcggga gatgctacgc
    10681 accataaatc ccacttcgtc catatgggcg cagatcatca ccttcggacc tgtcgattca
    10741 ttgaggcgga tcagcaccga tcccagacca tcaaatcgca cttctttttg caggcgatcc
    10801 gcttcttcca gcaggatctg ccgcacttcc tgttccgagg aggcgatcgc atctgcctcg
    10861 ctcaacgctt ttaatagcga taaatccatt atgcctctcc cgtgagcaac actgttttcg
    10921 gcatggcgta gagcacttct gcgccttgcg gggtaaccag cacaacatct tcgatgcgca
    10981 cgcccccttg ccctggcaaa taaatccccg gctccacggt cagtaacatg cctggctgta
    11041 gcgtcgtggt gtcccgcggt gaaaaacgcg gatcttcatg aacttcaatg ccgatagcat
    11101 gaccggtgtt atgaccgaaa tagtggctaa aacctgcctc ggtaatcact tggcgcgcgg
    11161 catcgtcaac ctgctggcag cgcacgccgg ggcgaattgc agagattgcc gcgagctgtg
    11221 cctgcaggac aatctgatag acgtcaaaca gcgggtgaga ttcggcgctc accccttcgc
    11281 cattcaccag caaggtgcgc gtcatatcag agcagtagcc ctgatacagc gcaccaaaat
    11341 cgagagtgac aaactcgccc gctgcaacaa tcttgtcgct ggctttgccg tgcggcagcg
    11401 ccccgcgcca gccgctggcg acaatggtgt caaaagaggc tttttctgcg ccctgctggc
    11461 gcataaacca ctccagttca gcggctatct cgcgctcgct catccccgcc tgaataaagc
    11521 ggcgaatatg ctccgctccg cgatcggcaa tcccacaggc gaggcggatt atctccacct
    11581 cctctggcgt tttgatttgc cgcagcacat ccggcgtggc gctaaccagt ttcgcattga
    11641 gttcagactt ccagcggtgc gcggtttccc aactcacctg ctggccttca aaaccgagcg
    11701 tttgcaactg ctcatcggca atgatttgat tgacgatagt ggtaagcgtg ttcgtcgcgt
    11761 caagcaaatg cagctggtag ccttgcgcac gggcttccac ctcaacgtaa tagcgcgaat
    11821 ccaccagaat gtgcgcactt tcacggctaa taaccacata gcctgatccg gtggagatcc
    11881 ccagatgcgg ctgtttgttc tgccgtgagg agagaagcac tgcatccagt tgttgcgcct
    11941 taagccagtc gcgcagcgaa gcgagtaatg tcatctggat tttccttctt acaggctatc
    12001 gattaacaat ttgcctttac ggaacatcat cagacgcagg aacaccacca tcagcgcagt
    12061 tatgactgcc cccagcgcga tccccgccat atagacgccc aggttagtga ccagcggcca
    12121 tgcccagata gcggactctg ggaaccattg cactgcgccc agccagacag cggcggttga
    12181 gccgacaata gcgccgacca tatacgacgg aatggcggtg atggggcttt ccagcgcaaa
    12241 cggaatcgca ccctcactga tccccataaa cgccaggaac atggcggtct taccctgcgg
    12301 ataaagttga gcgttgaaca ggcgtttgcc ggttaaacga cggtcgataa tggtcgccag
    12361 ccccagacca atcggcggaa taacgatagc gatagaacgc gcggtaaccg gtaatacgtg
    12421 gtcggtggta aagctgaagg caacgaaacc tgccgctttg ttgattgggc caccaaggtc
    12481 gattgcggtc gccgcggcaa tccccattgc atacatcagc gcgcccttct cacctgcggc
    12541 agtcagcacg gtacggatac cgccgttgat ccagccacca aatggggtga tgacatagta
    12601 catcgccagc atgacaaaaa tggcggaaag aatcggtagc aaaaatgtgg ttttgaacgc
    12661 taacaggaaa tctggtaact ggattttctg gttcatccat ttcacaaggt aaccagcaac
    12721 gatagatata atcagcgcac caatgaaggt ggacggcacc ggcgaagagg tcgcccattg
    12781 catagtgctg ggatcgaagt tcagcagttg cgtcggctgg gtagacatca agccgccgat
    12841 aaaaccagcc gggaacgcca gtttgccgcc gatagagttc gccacaaaag cggcaaacat
    12901 cggaatagca aagccaaaca acacgccgcc gaaggactgc gacagccagg caaatttcag
    12961 taaggagagg tcgaaaccgg agaatttccc gctattaaga gcatccatga tgccgatctc
    13021 agcaggaatt ttaagccagc tataagcaat cagctggctg aaagcgagga tcacaccgcc
    13081 cataattaag gtcggcacca tgcgtgaaat ccctgacatc acatgctgcg ggagttcacc
    13141 ccagaagctg cttttagagg cctgcggatt cttaactgcc gctgccgcac cggatgcgcc
    13201 aggcacaacg gttgcactgc gttttttaat ggccataaca attatccctg tcgaatatta
    13261 ctgttgttca gaggcaatca tctcttcgat ttctttgatg atgcctacag cgtttttaat
    13321 tgcgtcctgc aaagtgattt cataaacgtc gcgtgattcg aaacgttcgt tatcttccgg
    13381 ggtaactgcc acggaatgga tgatgatggt cgcttcggcg atatcctgcg ccgtcaggcg
    13441 attctggatg ccgtccgcgc cctgggtttc aattttcact tcataaccgg cttctaccgc
    13501 cgcttcttcc agcgcctgag cggccataaa agtgtgagcc aggcccatcg ggcaggcaca
    13561 taaggcaatc agtttcttac tcatcgttaa ttcctcgttc agtgatagtt gacgagtgaa
    13621 attatgaacg tgcaaaacaa atcgccgtta ccagacaaat aatgcattta ctggagaatt
    13681 aatcccgctg tatggaagtg tgacgcaggt cggccttgtg aggggacaca aagaaaaatt
    13741 gaattaactg tatgatttaa aagattatcg ggagagttac ctcccgatat aacaggaagg
    13801 atttacagaa tgtgacctaa ggtctggcgt aaatgtgcac cggaaccgag aagacccgga
    13861 ttgtcatgga cgatgagata caccggaata tcatggacat attctttaaa gcgcccttta
    13921 tcttcaaatg cggcacggaa accagaggct ttgaagaact caaggaagcg cggcacgata
    13981 ccgcccgcaa taaacacgcc gccaaatgtc ccgagattga gcgccagatt gccgccaaaa
    14041 cggcccataa tgacgcaaaa cagcgacaac gcgcggcggc aatcggtgca gctgtcagcc
    14101 agcgcgcgtt cggtaatatc ttttggcttg agattttctg gcaggcggtt gtcagctttc
    14161 acaattgcgc gatacaaatt caccagccca gggccagaaa gcacgcgctc cgccgaaaca
    14221 tgaccaattt ccgcacgcaa tatttcgagg ataatggcct cttcttcact attcggcgca
    14281 aaatcaacgt gaccgccttc gcccggcaag cttacccaac gcttatcgac atggaccaga
    14341 tgcgcaaccc caagccctgt tccggcaccg taaaccgcaa taggtttacc ttctaccggt
    14401 tctgcgccac caaactgaat cagatgctct tttttcagca tcgggatcgc catcgataca
    14461 gcggtaaaat cgttaataat ttccagatgg ctaaaaccga gattcttttt catttcggca
    14521 attgagaacg cccaggtatg gttggtcatt gccacccagt caccggtaat cgggcaagcg
    14581 atggcaatac agccgtcttt cacctcgacc ttatgttctt caagataaac gcgaatgacc
    14641 gcttcgaggc tggggtaatc aagccctgaa taggtcttag cctgcgagat ttcaccactg
    14701 gcaatatcac acagagcaag acgtgcgttg gtgccgccca catcaccgac taatgcatac
    14761 tttgtcattc ttcaactgct ccgctaaagt caaaataatt ctttctcaca ctgtaaatac
    14821 ctgggggcat aacaacaacg ccggaaaggc aggctccctg taaatatcga tctgggtcac
    14881 acaattactt tatcgtttca gcaccaattg cagcgatgcc tttttgcaag ctgggcaaac
    14941 taagtatctg accccgcata aggaatagaa catgctccat ccgcgagcca gaaccatgtt
    15001 gttattatcg ctccccgccg tggcaattgg gattgcgtcc agtcttattc tgattgtggt
    15061 gatgaaaatc gcctcggtat tacagaattt gctctggcaa cgactgccgg gaactctggg
    15121 gatagcccag gattcacctt tctggatcat cggtgtatta acgctaacag gtattgcggt
    15181 ggggttggtt atccgcttca gccagggtca tgccggacca gaccccgcct gtgaaccgct
    15241 gatcggcgca ccggttccac cttccgcgct accaggactt atcgtagcat taattctcgg
    15301 tcttgctggc ggcgtcagcc tggggccaga acatccgatc atgaccgtca atatcgccct
    15361 tgccgtcgcc attggcgctc gtctgttacc gcgcgtcaac cgaatggagt ggactatttt
    15421 agcctctgcc ggaaccatcg gcgcgctgtt tggtacacct gttgcggcgg cgttgatatt
    15481 ttcgcaaacc ttaaatggca gtagtgaagt tccgctatgg gatcgtcttt ttgcgccatt
    15541 aatggcggca gcagctggtg cacttactac cggattattt ttccatcccc atttttcact
    15601 gcccattgcg cattacggac agatggaaat gaccgatatt ctcagcggtg caattgtcgc
    15661 ggcgattgcc atcgcagcag gaatggtcgc cgtatggtgc ttaccacggt tgcacgcgat
    15721 gatgaatcaa atgaaaaatc cggtgctcgt gctgggtatt ggcggattta ttctcggtat
    15781 tctgggggtt attggtggac cagtttcgct gtttaaaggg ctggatgaga tgcagcagat
    15841 ggtggcaaat caggctttca gcaccagcga ttactttttg ctggcggtaa ttaaacttgc
    15901 cgccctggtc gttgctgccg ccagtggctt tcgcggtggg cgaatcttcc cggcagtgtt
    15961 tgtcggcgtg gcattagggt tgatgctgca tgagcacgta cccgccgtac cagcggcaat
    16021 aaccgtttct tgcgctattc tcggcatcgt gctggtggta acacgcgatg gctggttaag
    16081 tctttttatg gcggcggtcg ttgtacccaa taccacattg ctaccgctgc tctgtatcgt
    16141 catgctcccg gcatggctgt tattagcagg taagccgatg atgatggtca atcgtccgaa
    16201 gcaacagcca ccccacgata acgtttagca aaaaatgctc tcctttatga tttaagagtt
    16261 atggcgatga tacggggctt tacctccccg taatattgcg ttaacaggcc gctgacggct
    16321 tcgatcgtga aggagaataa caatgttcag gtcactgttt ctggcggccg ccctgatggc
    16381 atttaccccg cttgcagcaa acgcaggtga aatcacccta ctgccatcaa tcaaattaca
    16441 aattggcgat cgcgatcatt acggtaatta ctgggacggt ggtcactggc gcgaccgtga
    16501 ctactggcat cgcaattatg agtggcgtaa aaaccgctgg tggcgtcatg ataatggcta
    16561 tcatcgtggc tgggataagc gtaaagcgta tgagcgtggc tatcgtgaag gctggcgcga
    16621 tcgtgacgat catcgcggaa aaggccgcgg acatggacac cgccattaaa cacattcgcc
    16681 aatggaacac aatgcctgat gcgacgct
//
LOCUS       DAZFXT010000104        16545 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.104, whole genome shotgun sequence.
ACCESSION   DAZFXT010000104 DAZFXT010000000
VERSION     DAZFXT010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.104"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..96
                     /locus_tag="ACHKW0_003602"
     ncRNA           <1..96
                     /ncRNA_class="other"
                     /locus_tag="ACHKW0_003602"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(280..957)
                     /gene="narI"
                     /locus_tag="ACHKW0_003603"
     CDS             complement(280..957)
                     /gene="narI"
                     /locus_tag="ACHKW0_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7476369.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(957..1667)
                     /gene="narJ"
                     /locus_tag="ACHKW0_003604"
     CDS             complement(957..1667)
                     /gene="narJ"
                     /locus_tag="ACHKW0_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7476370.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASENLSKEDAHALG
                     IFLRDLTALDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLAQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVLFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(1664..3202)
                     /gene="narH"
                     /locus_tag="ACHKW0_003605"
     CDS             complement(1664..3202)
                     /gene="narH"
                     /locus_tag="ACHKW0_003605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7476371.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKAQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(3199..6942)
                     /gene="narG"
                     /locus_tag="ACHKW0_003606"
     CDS             complement(3199..6942)
                     /gene="narG"
                     /locus_tag="ACHKW0_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HFO7476372.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVMGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(7458..8849)
                     /gene="narK"
                     /locus_tag="ACHKW0_003607"
     CDS             complement(7458..8849)
                     /gene="narK"
                     /locus_tag="ACHKW0_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HFO7476373.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            9188..10984
                     /gene="narX"
                     /locus_tag="ACHKW0_003608"
     CDS             9188..10984
                     /gene="narX"
                     /locus_tag="ACHKW0_003608"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HFO7476374.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALIRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            10977..11627
                     /gene="narL"
                     /locus_tag="ACHKW0_003609"
     CDS             10977..11627
                     /gene="narL"
                     /locus_tag="ACHKW0_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HFO7476375.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(11628..13022)
                     /gene="ychO"
                     /locus_tag="ACHKW0_003610"
                     /gene_synonym="iatA"
     CDS             complement(11628..13022)
                     /gene="ychO"
                     /locus_tag="ACHKW0_003610"
                     /gene_synonym="iatA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HFO7476376.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            13207..13560
                     /gene="ychN"
                     /locus_tag="ACHKW0_003611"
     CDS             13207..13560
                     /gene="ychN"
                     /locus_tag="ACHKW0_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HFO7476377.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTHPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(13604..14299)
                     /gene="chaC"
                     /locus_tag="ACHKW0_003612"
     CDS             complement(13604..14299)
                     /gene="chaC"
                     /locus_tag="ACHKW0_003612"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HFO7476378.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGCTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(14457..14687)
                     /gene="chaB"
                     /locus_tag="ACHKW0_003613"
     CDS             complement(14457..14687)
                     /gene="chaB"
                     /locus_tag="ACHKW0_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HFO7476379.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            14957..16057
                     /gene="chaA"
                     /locus_tag="ACHKW0_003614"
     CDS             14957..16057
                     /gene="chaA"
                     /locus_tag="ACHKW0_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HFO7476380.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            16462..>16545
                     /locus_tag="ACHKW0_003615"
     CDS             16462..>16545
                     /locus_tag="ACHKW0_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7476381.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIV"
BASE COUNT         3996 a         4440 c         4155 g         3954 t
ORIGIN      
        1 caaggttcga cttgagcacc agcgagagag cgttgccgca ggcaacgacc cgaagggcga
       61 agcgcgcagc gctgagtaat ccttccccca ccaccatcgc ttccttaaat aatcaaaacc
      121 tgttactccg agctaatttt gcccgatggc accacgttta ccgggcctac aatatcctcc
      181 cctgtaggtc ggataagcga agcgcatccg acaactcacc ccgcaccgtg cccataaaaa
      241 aaccccgccg aagcagggtc tatgtgaact aaaattcgct tagtgacgag cgcgcaccag
      301 ctggtactta cgtgtcagat actccaccgg tacgctccag atgtgtacca gacgcgagaa
      361 cgggaacagc aggaataacg tcatccccag caccaggtgc agacggaaga taaacgccac
      421 accatcgagg tgttgagaag cgccaccgtg gaaggtcacc accgactgcg cccagccaac
      481 cagtttcatc atctcgctac cgtccatatg ctgagcggag aacggaatgg tcagcaggcc
      541 cagcgcgcac tggataacga gcagcgacag gatcaggata tccgctccag tagtggttgc
      601 acgcacgcgt gggctgaaca gacgacgctt cagcaacagc acgccgccaa tcagacacag
      661 cacgccgctg gcaccaccag caaacatggc cattttctgt ttcacttcaa tcggcagcca
      721 ggcttcatac atccagtgcg gcgtcaacat accgaagaag tgaccgacaa aaatccccag
      781 aataccgata tggaacaggt tcgacgccag gttcatccct ttgcgatcca gcatctggct
      841 ggacgccgcg cgccaggtgt actgcccgta gtcataacgc agccagctac caatcaggaa
      901 gaccgccccg gctatgtacg ggtagatatc aaagaagaac atattcagga attgcattag
      961 tgctgtcctc cggtggtgat attcagatat tgcggcgcga cggcaccggc aaagcgacgc
     1021 tgatgagcag tgattgctga atcaccgcag cctttgtcag caaagaattt aacctgctct
     1081 tcttcccaga cagcatccag cgcctgcggc gtatcatcgc gcgcttcgtc ggcaattttt
     1141 tccgccactt tgtcgctgtc gatagcggta ttggccaatt tcagcagcag atcaaacagc
     1201 acggcataac ggctttcacg ctgttgcaga cgcgcgctca gcaatgccag aatcggcgcg
     1261 atatctttca aaccttccac ggcttcgctt tgcggcagct gagccaggta ctccagatac
     1321 agcggcagat ggtccggcaa ttcgcggctg tttaactgca agccgtgctg ctcgtactgc
     1381 gccaacaggt caaccatcgc ctggccgcgg tcgcgggatt cgccgtgcac atgttcaaac
     1441 agcaacagcg acgtggcgcg accacggtcg aacagttcgc tgtactgcgc ctgggcatcc
     1501 agcggatcca gcgccgttaa atcgcgcagg aaaatgccca gcgcatgggc atcttctttc
     1561 gacaaatttt ccgacgcggc aatcgcctca aacatctcct gttgatgctg ccataaggca
     1621 gcatccggat attcaaggag acgcgataca atcacgagtt cgatcatgga tgcggctccg
     1681 ttttgctggt cacatcgatg gcatcgatac gacggctgtt gaacagattg aatttggtat
     1741 ctgagccgtg gcagccatca ccaaaggtaa agccgcagcc atttttctcc gggaaggctt
     1801 cccgtgccag ttcacgatga ctactcggca ccacaaagcg atcttcgtag ttagcaatcg
     1861 ccagataacg gtacatctcc tgtgcctggg cttcagtcag gccgacctct tccagcgcac
     1921 gggtatcaac tttaccgtca acggtttcag cacgtttgta atgacgcatc gccagcatac
     1981 gtttcagtgc gcgcagtacc ggtttggtat caccggcggt cagcagattc gccagatact
     2041 gtaccggaat acgcaagctt tcgacgtctg gcagaattcc gttgctgccc agctcgcccg
     2101 cgtctgctgc agactgaatc ggagacagag gcggcacgta ccagaccatc ggcagtgtgc
     2161 gatattctgg atgcagcggc agcgccagtt tccattccat cgccatttta taaaccggcg
     2221 actgctgtgc agcttcaata acgctcagcg gaataccgtc tttaatcgcc tgctcgatga
     2281 ctttcggatc gttcggatcg aggaacacgt ccagctgacg ctggtagaga tctttctcgt
     2341 tctcggtgct ggctgcacgt tcaatcgcgt cggcatcgta cagcagcacg ccaagataac
     2401 ggatacgacc gacacaggtt tctgagcaca cggtcggctg acccgcttca atacgcggat
     2461 agcagaagat gcacttctca gacttaccgc tcttccagtt gaagtagatt tttttgtacg
     2521 ggcatccggt gatgcacata cgccagccac ggcatttatc ctggtcgatc aggacgatgc
     2581 catcttcttc acgcttgtaa atcgcaccgc tcgggcaggt cgccacacat gccgggttca
     2641 ggcagtgttc gcacaggcgc ggcaaataca tcataaaggt gttttcgaac tggctataca
     2701 tcgccttctg gatgttgtcg aagttcttgt ctttcgccag tttgtcaaac tcaccgccca
     2761 ggtcatcttc ccagttcggc cctttttcga ttttcgccat ccgttccccg gtaatcagcg
     2821 aacgcggacg ggcaatcggc tgcgctttgc tgccttccgg cgcggtatgc aggttctgat
     2881 agtcaaaatc gaacggctcg taataatcgt cgatccccgg cagatgcggg ttagcgaaga
     2941 ttttacccag cagcatggcg cggttaccca tgcgcggctg cagtttgcca ttgattttac
     3001 ggatccagcc gcctttgtat ttttcctggt tttcccagtc agtcgggaag ccctggcccg
     3061 gcttggtttc cacgttgttg aaccacgcgt attccacgcc ttcacggctg gtccagacgt
     3121 ttttacaggt aactgaacag gtgtggcagc cgatgcactt atcgagattc agcaccatgc
     3181 cgacttgtga acgaattttc attttacgct ctcctgtacc tggtcattgc cttcgccatc
     3241 taaccagtca atgttcttca tcttacgcac tacaacaaac tcatcgcggt tagaacctac
     3301 ggtgccatag tagttaaagc cgtatgccag atgggcatag ccgccgatca tatgcgtcgg
     3361 tttcggcgtg atacgggtga ccgagttatg gataccacca cgctgttggg taatttccga
     3421 accaggcagg ttaacgatac gttcctgcgc gtggtacatc atggtcatcc ctgccggaac
     3481 acgctggctg acaaccgcac gggcagtcag agcaccgttg ctgttgaata cttcaatcca
     3541 gtcgttatcg gcgataccca gatctttggc atcggcttca ctcaaccaga ccaccggacc
     3601 accgcgacct aaagtcagca tcagcaggtt gtcgctgtag gtggagtgga taccccactt
     3661 ctggtgcggc gtcaggaagt tgagcgcttt ttccgggttg ccattggatt tctggcccat
     3721 cacttctttc accgaacggg tgtcgatcgg cggacgataa accagcaggc tttcaccgaa
     3781 atcacgcatc cactggtgat cctgatacag ttgctgacga ccagagagcg tacgccacgg
     3841 gatcagctcg tgaacgttgg tgtaaccggc gttgtaagaa acgtgttcat cttccagacc
     3901 agaccaggtc gggctggaga tgattttgcg cggctgtgcc tgaatatcgc ggaagcgaat
     3961 cttctcgtct tctttattca gcgccagatg cgtatggtca cgaccggtaa actcgctcag
     4021 ggcagcccag gctttcacgg ctacctgacc gttggtttcc ggtgccagcg tcaggatcat
     4081 ctctgccgca tcaattgcgg tgttcagcat cggctggcct tttgccggac cttccgcttt
     4141 ggtgtagttg agcttacgca gcagatccat ctcgctctgg gtgttccagg caataccctt
     4201 accaccgtta ccgattttct ccatcagcgg gccgatagag gtaaagcgtt cgtaagttgc
     4261 cggataatcg cgctctacga ccataatgtg tggcgcggtt ttacctggga tcaggtcgca
     4321 ttcgcctttt ttccagtctt tcacatccag cggctgcgcc agttcagcgg cagagtcgtg
     4381 ctggataggc agcgtgacga tgtcggtttc tttacccaga tggccgacgc acacttcgga
     4441 gaatttcttc gcgatggctt tgtagatttc ccagtcgctt ttcgcttccc aggccggatc
     4501 gaccgccgca gacagcgggt gaataaacgg atgcatatcc gaagtattca tgtcgtcttt
     4561 ttcgtaccag gtcgccgtcg gcaaaatgat gtcggaatag agacaggtgc tcgacagacg
     4621 gaagtccagc gtaaccacca gatccagctt gccttccaga ccattgtcct gccagtccac
     4681 ttcttccggc ttcacgccgc cctgctgccc cagatcttta ccctggatac cgtgctccgt
     4741 ccccagcagg tacttgagca taaactcatg acctttaccg gaagaaccga gcaggttaga
     4801 acgccagatg aacaggttgc gcgggtggtt tttaccgttt tctggttgtt ctgccgcaaa
     4861 acggatggaa ccctctttca gggatttcac ggtgtaatct accggattca tcccggcttt
     4921 ttccgcttcg ccagcgatag tcagcgggtt agtgcctaac tgcggtgcag acggcagcca
     4981 gcccatgcgt tctgcacgga cgttaaagtc gattaggtgt ccggtataac gggatttatc
     5041 cgccatcggc gacagcagtt cttccgcagt cacggtttca taacgccact ggctggagtg
     5101 gttatagaaa taagaagtgc tgttcatgtg acgcgccgga cgctgccagt caagggcaaa
     5161 cgccagcggc tgccagccgg tttgcggacg cagtttttcc tgacctacat agtgcgccca
     5221 gccgcccccg ctctgaccga cacagccgca gaaaatcagc atgttgatca gaccacgata
     5281 gttcatatcg aggtgatacc agtggttcag ccccgcaccg acgataatca tcgaacgacc
     5341 gtgcgtttta tcagcgttat cggcaaattc acgggcgatg cgaataatct ggctgcgaga
     5401 aacgccggta atctgctcgg cccaggccgg ggtataagct ttcacatcgt catagctggt
     5461 tgcacagtta acgtcgttca ggccacgttc cagaccgtag tttgccagcg tcagatcata
     5521 aacggtggtc accagggcgg tgctgccatc agccagttgc aggcgtttca ccggcagttt
     5581 gtgcagcagc acgttttcca gttccacttt gttgaagtgt tcagtaccgt cgccaccaaa
     5641 gtacgggaag cccacctcag cgatctcatc ctggctaccc agcaggctca gttgcagctc
     5701 ggtttcttcg ccagttttgc cgtcgcgctg ctcaagattc catttgccct tctcgcccca
     5761 gcggaagcca atagaaccgt tcggcgcaac catttcacca ttggtattaa aggcgacagt
     5821 tttccattcc ggattgtttt cctgacccag cgcatccacc agatcagcag cgcgcagcat
     5881 acgacccgcg gcgtagtaac cgtcgcgctc ttccagcatc accagcatcg gcatgtcggt
     5941 gtagcgacgc acatagtcgg tgaaatactg gcttgggttg tcgaggtgga attcacgcag
     6001 cattacgtgg cccattgcca gcgccattgc cgcatcggtg ccctgtttcg gtgccagcca
     6061 cagatcgcac agtttggcga tttcagcgta gtccggcgtg acggcaacag ttttggtccc
     6121 tttgtaacgc acttcagtaa agaagtgagc atccggggta cgcgtctgcg gcacgtttga
     6181 cccccaggcg atgatgtagc tggagttgta ccagtcagca gattccggta cgtctgtttg
     6241 ctcgccccag gtttgcggag acgcaggagg caggtcgcag taccagtcgt agaagcttaa
     6301 gcaagtaccg ccaatcagcg agagatagcg tgcacccgat gcgtaagaaa ccatcgacat
     6361 tgccggaatt ggcgagaagc cagcgacacg gtccgggccg tagtttttga tggtgtaaac
     6421 gttagatgcg gcgatcagtt cgttcacttc ctgccaggaa gaacgaacaa aaccaccgcg
     6481 tccacgcgcc tgcttaaagc ttttcgcttt atcggcgtct tcaatgatag aagcccatgc
     6541 ctcaaccgga tcgctatgca gcgccttcgc ttcacgccac attttcatca agcgtttgcg
     6601 catcatcggg tatttcaggc ggttggcgct gtaaagatac caggagtagc tggcaccgcg
     6661 cgggcagccg cgaggttcat ggtttggcag gtccgggcga gtacgtggat agtcagtctg
     6721 ctgggtttcc caggtgacca gaccgttttt gacgtagatt ttccagctgc aggagccggt
     6781 gcagtttacc ccgtgggtag agcggacgat tttgtcatgc tgccaacgct ggcgatatcc
     6841 atcctcccag tcacggttgg tattgagaag ctggccatgc ccatcggcaa aggtttcacc
     6901 cttctgtttg aagtagcgaa accggtccag gaatttactc atcggttttc tcctgtggga
     6961 gcctgtcggc cctctgataa atcgacattg ctaatgttta agggcaaggt aacgctctga
     7021 aacagcgtgg gaattgataa cgatcaagtg tgatggggat gaaaaataaa gtaaattcct
     7081 aagatacccc tttaggataa ttaacagtta aagcataaca tgctgtaaat taagacgttt
     7141 tacacctgta gttatttaaa ctattaagaa ttttatgcag atgggtatta aggagtattc
     7201 cccatgggta acatattaat ggctcttaat gaaatccggc gaaataaacc atacaacatt
     7261 agtatgttgt aactaaatga cgcataaaaa aagcgcgaca tcatgccgcg caaaggataa
     7321 tcaaatattt tactctatat aattcggttt acaggaaggc ggcaagtgaa tgaatcccag
     7381 gagcttacta aagtaagtga ctggggtgaa tgaacgcagc cgcagcacat gtaacccgaa
     7441 gtatgacgag taacggttta ttttttagaa tgccgaccat ataccgccca ggtaatcact
     7501 acgcaggcga tatagaaaat caaaaatact ttcattgcgc cgactggcga acccgttaat
     7561 gccagcgagc taccaaacgc tttcgggata aagaagccac caatcgcgcc aatcgcagag
     7621 atgaaaccca acgccgccgc cgtgtcggtt gccgcttcac gcatcgcacg ttcgtcagaa
     7681 cccccttctg ctttcacgcg atccattgtc agtttacgga agatcactga aatcatctgg
     7741 aaagtggaac cactacccag cccagctgtc aggaacagcg ccaggaagac cgcgaagaac
     7801 gccataaagc ttccgccctg cccgtcagtc ggtaaggtca ggaacagcag gccgctgaaa
     7861 atcgccatca gaataaagtt caccagcgtg acacgagttc cgcccagacg gtcagataat
     7921 gcaccacctg cagaacgcgc cagcgcacca ataaacggcc cgaagaaagc gtattgcaga
     7981 atctgaacat ccgggaactg cgtttttgac agcatcgcaa agcccgcgga gaagccgata
     8041 aaggagccga aggttgccag atacagcagg ctcataatcc acagatgccc ccgtttgagt
     8101 accggcaact gctccttgat ggaggctttc gaggtagcaa gatcgttcat gccaaaccac
     8161 gccgcaatgg tgaagatggc aaggaacggc acccatatcc aggacgcatt cgccagatac
     8221 agctcagtcc catccggctg tttgacgccc tggctaccaa atactgcgaa aatcgacagt
     8281 gataccacca gcggagcaac caactgcatg acgctgacgc ccatgttgcc cagaccacca
     8341 ttcagaccca gcgcgccacc ctgcttctgt ttcggaaaga agaagctgat gtttgccata
     8401 ctggaggcga agttcgcgcc agcaaagccg cacagcagag agatgatgat gaagacgcta
     8461 taaggcgtgg aggtatcctg cacggcaaaa cccagccaga cacaaggaat aatcagaata
     8521 ccggtgctga acgccgtcca gcgacgacca ccgaagatag gaaccataaa ggagtatgga
     8581 acacgtaata acgcgccaga aactgaaggc agcgcagtca acataaatag ctgatcggtc
     8641 gtaaaattaa aaccgacttt cggtaagttc accgcaacag cgctgaacaa catccataca
     8701 caaaacgcca gcagcagaca gggaacggaa atccacaggt tgcggctggc aatacgttga
     8761 ccgcgttgtt gccagaacgc aggatcttcc ggtcgccaat ctgtaatgac agctccagta
     8821 gccctttcgg gggcggatga gtgactcata gacacctctg atactcgttt cgatgtctgc
     8881 caccttagtg tctgtagcta aaggcaattt gatgtaaatc aaacgataag ataactttat
     8941 ctatcattga tatttatcat tacccatagt gagtacagtg atttcataaa aattatgaga
     9001 tttttcacgg tgctgtaaaa tccctaccct taccgatgta aagcgactaa ccacacggca
     9061 aataaggagt aactccttcc gggtatgggt atacttcagc caatagccga gaatactgcc
     9121 attccagaat gtatcgtcac attcattaag gttattgctc atttaaagcc tgaaggaaga
     9181 ggtttacatg ctcaaacgtt gtctctctcc gctcaccctg gttaatcagg ttgcgcttat
     9241 tgtgttgctt tctactgcta ttggactggc agggatggcg gtttctggct ggctggtgca
     9301 aggcgttcag ggcagcgccc atgcgatcaa caaagcggga tcgctgcgca tgcaaagtta
     9361 ccgtctgttg gcagcagtgc cattaagcga gaaagacaag cccttaatta aagagatgga
     9421 acaaacggca tttagcgccg agttgactcg agcagcagaa cgagacggac aactggcgca
     9481 attacagggt ttacaagatt actggcgtaa tgaactgatc cctgcgctga tacgtgcaca
     9541 aaaccgcgaa acggtgtcag cggatgtcag ccagtttgtt gccgggcttg atcagctggt
     9601 atctggtttt gaccgcacca cggaaatgcg cattgagaca gtggtactgg tccatcgggt
     9661 aatggcggta tttatggcac ttttactggt gttcactatt atttggttgc gggcgcgact
     9721 gctacaaccg tggcggcaac tgctggcaat ggcgagtgcc gtcagccatc gcgattttac
     9781 ccaacgcgcg aacatcagcg ggcgcaacga aatggcgatg cttggaactg cattgaacaa
     9841 tatgtctgca gaactggccg aaagttatgc cgtacttgag cagcgggttc aggagaaaac
     9901 cgccgggctg gagcataaaa atcagatcct ctctttttta tggcaggcta accgccgttt
     9961 gcattcccgc gccccgctgt gtgaacgcct gtcacctgta ctcaacggct tacagaattt
    10021 aaccctgcta cgtgatatcg aactgcgggt ttatgatact gacgatgaag agaatcatca
    10081 ggagtttacc tgccagccag atatgacttg tgatgataaa ggctgccagc tctgcccgcg
    10141 cggcgtatta cccgttggcg atcgcggcac gaccctgaag tggcggctgg ctgactctca
    10201 tacgcagtac ggtattttgc tggcgaccct gccgcagggg cgtcatctta gccatgatca
    10261 acaacaactg gtggataccc tggttgaaca actcaccgcc acgctggcgc tggatcgcca
    10321 tcaggaacgt cagcaacagt tgatcgtgat ggaagagcgt gccaccattg cgcgcgaact
    10381 gcatgattct attgcccaat ctctctcttg catgaagatg caggtgagtt gtttacagat
    10441 gcagggcgat gcgctgccag aaagcagccg cgaactgtta agtcagatcc gtaacgaact
    10501 gaatgcatcc tgggcgcagt tgcgtgaatt gctcaccaca ttccgcttgc agctcaccga
    10561 gcctggatta cgtccggcgc tggaggcgag ttgcgaagag tacagcgcca aatttggctt
    10621 cccggtgaag ctggattatc aattgccgcc tcgtctggtg ccttcgcatc aggcaatcca
    10681 cttgttgcaa attgcccgtg aggcattaag taacgccctc aaacattcgc aagcgagtga
    10741 ggtcgtggtg acggtggcgc aaaacgataa tcaggtaaaa ctgaccgttc aggataatgg
    10801 ctgcggcgtg cctgaaaatg ccatccgcag caatcactac ggcatgataa taatgcgcga
    10861 ccgtgcgcaa agtttacgag gcgattgccg cgtccgccgt cgtgaatcag gtggcaccga
    10921 agtggtggtc acctttattc ccgaaaaaac tttcacagac gtccaaggag atacccatga
    10981 gtaatcagga accggctact atcctgctga ttgacgatca cccgatgctg cgaactggcg
    11041 taaaacagct tatcagtatg gcaccagata tcaccgtggt tggcgaagcg agtaatggcg
    11101 aacagggtat tgaactggcg gagtctcttg atcccgatct gatcctgtta gatctcaata
    11161 tgcccggcat gaacggtctg gaaacgctgg ataaactgcg cgaaaagtcc ctctcagggc
    11221 gcattgtggt attcagcgtc tctaaccatg aagaagatgt ggtcaccgca ctgaaacgcg
    11281 gcgcggatgg ctatctgtta aaagatatgg aaccggaaga tctgctgaaa gcattgcatc
    11341 aggcagctgc tggcgaaatg gtattaagcg aagcattaac gcctgttctg gccgccagct
    11401 tgcgcgctaa ccgtgccact actgagcgcg atgttaacca gttaacccca cgcgagcgcg
    11461 atattctcaa gctgattgcc cagggtttgc cgaacaagat gattgcccgc cgcctggata
    11521 tcaccgaaag cacagtaaaa gtgcacgtca agcacatgct gaagaaaatg aagctcaagt
    11581 ctcgcgtgga agcagcggta tgggtgcatc aggagcgcat tttctgatta cggttcccag
    11641 cgcagttcgt cgtttgacaa tgcatcgaac ggttcgacaa gcgttagcgt gatctcattg
    11701 gaggagacac gctgcccctg gttatcttcc accaccaccg ataatcgcca gtgattgctt
    11761 gccccttccc cgttctgcca gtcaggcatg atcagcgtcc agccctccgc gctgttggct
    11821 tgtgcgcccg gcgtcaaact taatatctgc gtatcgccct gccaaatcag ttgccgaata
    11881 ccgtaacgac tgcggatttg taatttcagc ggcactgttt cgccaggttt tagatcccac
    11941 ggcggtgtcg ccagaaacac cgttaacgtt tttcgctgtc ggtactcaag agtcggtaga
    12001 ttatttcgct gcggattgtc atagcgacta ccacgtaacg actgactttc ggcaacctcg
    12061 cccgcagaaa gttgtttttt gagcggtaca ccaaagcggt aattaagatt cagcccgagg
    12121 ttattttgat tctcgccact ttcaccctgt ttatgctggg ccgtcacagt gactaatggc
    12181 acaggggtgt aatttaatcc cagactcaac gcgacgggat tgtgataacc cgtaccagag
    12241 ttaaacaaat caacacgatc accaaaatac tgttctacgc tgacactggt attgaggtgt
    12301 tgatagaacg gcatgcgcat ccgggctgtc aggtcatacc cgcgcgccat ccgttgttcc
    12361 tgcgtggctg tctgttcatg ccatgcagca aacggctgat aaaagtttgc cgatagtcgc
    12421 aaatattcgc cccacgcttc ggcaccaaag cccgctcgct gaagattttc gtccagcaag
    12481 ttgtcataaa aagtgttata acccaccagc cagttgccgc gcgcccagcg ttgcccaacg
    12541 cccacattgc tcaccaaccc atcatcctgc tgagtaagac caagctggct ccaggtgaga
    12601 taacgatcat tatcttgtaa cggcacaaac cagcttccac gactgccggt gaaatgtcct
    12661 tcgttatcca ctttgacgtc aacactggca tttccccacg gtgatagcca ggactctacg
    12721 tgctgattaa cctgttgact aagcgcgtcg cggacttttc ccaatgcgaa agcttttgcc
    12781 tgctcgccag tatccagccc gttatcattc atactggttt cgccgaaatc tttcacaatt
    12841 tcagcaaagt gtttttcccc atcatgattt tcgggagcca tgcctaaatc cggcagacca
    12901 tcattgttat tatcaaaggg atttgccgct ttttgctcga aggtagattg tgcgttagct
    12961 gtaccgcctg ctacaagcaa gagtaaataa aacgggataa tgcgaggaac gaaacggctc
    13021 aaaacgtcgg taacttccgg taataatgaa aaaccatagc cttaaagaat aacgattact
    13081 ggcaactttt gtcgtatttt ctcgcgcttt caggaaaatc cttaaaaatg tgaccgattg
    13141 gtcagacgct gcagaagcag taagatagaa gatgaatgtc taattccctg aatacaggag
    13201 taaataatgc aaaaaatcgt gatcgttgcc aatggcgcac cttacgggag cgaatccttg
    13261 tttaacagct tgcggctggc cattgcgtta cgagagcagg agagcaatct ggatctgcgt
    13321 ctgttcctga tgtctgatgc ggtcacagcc gggttgcgcg ggcaaaaacc aggggaaggc
    13381 tacaacattc agcaaatgct ggagatcctt accgctcaga atgtaccggt gaaattgtgc
    13441 aaaacctgta ccgacgggcg cgggattagc acacatcctc ttattgatgg ggtggaaatc
    13501 ggtactcttg tggaactggc gcaatggacg ctgtcagccg ataaagtgct cacattttaa
    13561 tgtttgaggg ggcgtctgac gccccactat gagggaagtt tactcaggcg aatcccggcc
    13621 gtaacacacc atccggataa ttctccgcca gcagtttttt taccgatacc agcaaatcat
    13681 tcagcccatc gtcctgcatt cccagtttga taagctcctg ttccagtgaa aacaggtatt
    13741 gtgcgttggt tcccagcgga ccgctcgccg ccgcaatcaa cggcgcgatg acctgagcgc
    13801 gagtgtcaga ttcatattct ggatgtcgcg ggtccataat aaacacaatg gcgtttactg
    13861 tgcatccatc atcaagatca agctgacacc aggttggcag ataacagccg gtaatcatct
    13921 cgcgcttcca caacagggtt agttcctgct ccagcgtctc ttctggcagt cgataggcga
    13981 cgcctgtagt gcgtccgccc tctttcagtg caagcatccg tcccggctgg tgcgcagtcc
    14041 cgcgcccggc ggtcaggcgc aggcaaaacg cgcgatgcca tccaaccagt gtaccggtgc
    14101 acgactcggt aaactccagt gccggattcc acatcaacga tccataaccg aagatccaca
    14161 ccggtccctc atcaggtcga caagccagcg tcgccgccag cgaagccgcc cgttgctctg
    14221 ctgaccataa gagtgattct tcaatggcac caaatgccgt tttacaatcg gcattcatca
    14281 agaaatcacg cgttatcacc ttacacctcc gccactgcat acttccctgc gacaactttt
    14341 tggtgtcttc cggacatttt tactgctcat tttgaaacca ctaacgggac aatatgcaat
    14401 ccacggacca cttgcaaggc aacaccgcac gaatagcttt aactaagcta ccggttttac
    14461 gattttttat gccatttatc atcatcccct ttggcatatt catgcttcac agcagcccag
    14521 gccactttat gcgcggtttc ttcgcgactg gcgtcatcac gccgatcgtc tttatcttta
    14581 tattgatccc atgcgctgtt gaacgcttct ttatagatat cctgggcatg agacggtaga
    14641 acgtgcttta cgctttccgg cagatcgctt ttcgttttat acggcatcgt tgactccttt
    14701 tacaaagttg aacattaagt gtggtcaaca atatctactc ttgccaaaac ataagaattt
    14761 tcatgtcaga ctgttcttaa gaaacgctaa aacaaacatt gtctaaacgt aatacttatt
    14821 tttatgtccc aatgagtaaa aaatcgacag tggggtaaaa attcgtaaaa tgttaccgat
    14881 atccagacac tttctgttat ttttcctacc tattttttac attttcgata aattcatctg
    14941 caaagggata accataatgt caaatgctca agaggcggta aaaacccgcc acaaggagac
    15001 ttcgcttatt ttcccggttc tggcgctggt agtgctgttc ctgtggggaa gcagccagac
    15061 actaccagtg gtcattgcca tcaatcttct tgcgcttatt ggtattttaa gtagcgcctt
    15121 tagtgttgtc cgtcatgcgg acgtattagc ccatcgcctg ggagaacctt acggttcgct
    15181 tattcttagc ctttcagtgg ttattcttga agtcagtttg atttcagctt taatggcaac
    15241 cggcgacgcc gcgccaacgc taatgcgtga tacgctctat tcaatcatta tgattgttac
    15301 cggtgggctg gttggctttt cattattgtt gggcggtcgt aagtttgcca cccaatatat
    15361 gaatctgttt ggtatcaagc agtatttaat tgcgctgttc cccctggcga taatcgtact
    15421 ggtatttcca atggctctgc ctgcggcgaa tttttcaacc ggtcaggcgt tactggtagc
    15481 attaatttct gcggcaatgt atggcgtatt tttgctgatc cagaccaaaa cgcatcaaag
    15541 tttgtttgtc tacgagcacg aagatgacag tgatgacgat gacccgcatc acggtaaacc
    15601 gtctgcccat agcagcgtgt ggcatgctat ctggttgatt atccatctga ttgccgttat
    15661 tgcagtcacc aaaatgaatg ccagcccgct ggagacattg ctcgacagca tgaatgcccc
    15721 tgtcgccttt actggcttcc tggtggctct gttgattctg tcgccggaag gtttaggtgc
    15781 gttaaaagca gtgttgaaca accaggtcca gcgcgcgatg aatctgttct ttggttcagt
    15841 gttagcaacc atttcgctta ccgtacctgt cgtcacgcta attgccttta tgacgggtaa
    15901 cgaattacag tttgcacttg gtgcgccaga aatggtggtg atggtggcct ctttagtgct
    15961 gtgccatatc tccttctcca ccggacgtac taacgtgctc aatggcgcag cgcatctggc
    16021 actgtttgcc gcctatttga tgacgatatt tgcctgacag ttatatctcc tccgatgacg
    16081 gaaatcgggg gagataagat aaattcctgc ttattgtatt tcttcatact acctggacag
    16141 ctcgttaatc agaattgcga gcctgtacca caacctgcac gaaaccactg caacagctgt
    16201 tgaaaagccg gaatgtactc tttattgcaa acatttcagg gatgaagtga agatgaattg
    16261 agacgtagta tccacaccaa gccggaatta tccggtgagg cgcaatgttg cgggggcttt
    16321 atccctggtg gcattggttg ctggaaagag aaaacccccg cacgttgaga ggtaaaacct
    16381 gacaacagaa cgggggctaa tcttgaaacc agacacttca agaatagccg caaaatgttg
    16441 tcattacaac aaggcggcta tatgacgctc gcgcagtttg ccatgacttt ctggcacgac
    16501 ctggcagcac cgatcctggc ggggattatt accgcagcga ttgtc
//
LOCUS       DAZFXT010000105        16121 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.105, whole genome shotgun sequence.
ACCESSION   DAZFXT010000105 DAZFXT010000000
VERSION     DAZFXT010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16121
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.105"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(125..1774)
                     /gene="pgi"
                     /locus_tag="ACHKW0_003616"
     CDS             complement(125..1774)
                     /gene="pgi"
                     /locus_tag="ACHKW0_003616"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HFO7476382.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FGDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     regulatory      2011..2207
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            2299..3648
                     /gene="lysC"
                     /locus_tag="ACHKW0_003617"
     CDS             2299..3648
                     /gene="lysC"
                     /locus_tag="ACHKW0_003617"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HFO7476383.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3793..4719
                     /locus_tag="ACHKW0_003618"
     CDS             3793..4719
                     /locus_tag="ACHKW0_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HFO7476384.1"
                     /translation="MATLMEKDALLNGASQCITFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4755..5546)
                     /locus_tag="ACHKW0_003619"
     CDS             complement(4755..5546)
                     /locus_tag="ACHKW0_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HFO7476385.1"
                     /translation="MPLNGVSAAMRERVSQQLREIERRYDVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEAPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPKWFSPLRARWHYYSMARKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5552..6679)
                     /locus_tag="ACHKW0_003620"
     CDS             complement(5552..6679)
                     /locus_tag="ACHKW0_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HFO7476386.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHILPWFTTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVERYCVVADLSETEAGALYMDGILQEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVDAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
     gene            7208..7480
                     /locus_tag="ACHKW0_003621"
     CDS             7208..7480
                     /locus_tag="ACHKW0_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HFO7476387.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDHARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAAAKKARQLKKKQAYKPDTEASFNWSANTPTHGRR"
     gene            complement(7481..8353)
                     /gene="rluF"
                     /locus_tag="ACHKW0_003622"
     CDS             complement(7481..8353)
                     /gene="rluF"
                     /locus_tag="ACHKW0_003622"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HFO7476388.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPLVKMEKTSEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            8565..9254
                     /gene="pepE"
                     /locus_tag="ACHKW0_003623"
     CDS             8565..9254
                     /gene="pepE"
                     /locus_tag="ACHKW0_003623"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HFO7476389.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(9345..10976)
                     /locus_tag="ACHKW0_003624"
     CDS             complement(9345..10976)
                     /locus_tag="ACHKW0_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HFO7476390.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGGEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(11196..14879)
                     /gene="metH"
                     /locus_tag="ACHKW0_003625"
     CDS             complement(11196..14879)
                     /gene="metH"
                     /locus_tag="ACHKW0_003625"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HFO7476391.1"
                     /translation="MSSKVEQLRAQLNQRILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVTEVERWLAPNLGYDAD"
     gene            15079..15903
                     /gene="iclR"
                     /locus_tag="ACHKW0_003626"
     CDS             15079..15903
                     /gene="iclR"
                     /locus_tag="ACHKW0_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HFO7476392.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDSVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         3879 a         4309 c         3988 g         3945 t
ORIGIN      
        1 tcacgattaa aaatctattt taggattaac ctcacggtat gatttccgtt aaatcacaga
       61 caaggggatt tatctgataa aacaatgccg acataacgtc ggcattgtta ttaaggtttt
      121 taaattaacc gcgccacgct ttatagcggt taatcagacc attggtcgag ctatcgtggc
      181 tgctgatttc tttatcatct ttcagctctg gcagaatacg gtttgccagt tgtttaccca
      241 gctccacgcc ccactggtcg aaggtgaaga tgttcaggat cacgccctga gtaaagattt
      301 tgtgctcata cagcgcaatc aacgcaccca ggctgaacgg agtgatttca cgcagcagga
      361 tggagttggt cgggcggtta ccttcgaaca ctttgaacgg caccacgtag tcaagcgttg
      421 ccggatcttt accctgatca cgatattcct gctcaaccac ttcgcgggat ttaccaaacg
      481 ccagcgcttc ggtctgggcg aagaagttag acagcagttt ctggtgatga tcagagagcg
      541 ggttatgggt gatagccgga gcgatgaaat cgcacggtac cattttggtt ccctggtgga
      601 tcagctggta gaacgcgtgc tgaccgttag tgcctggttc accccagata atcgggccag
      661 tctggtaatc cacaacgtta ccgttacggt caacatactt accgttggac tccatattgc
      721 cctgctggaa gtacgccgcg aaacggtgca tatactggtc atacggcaga atcgcctcag
      781 tttccgcacc aaagaaattg ttgtaccaga tgccaatcag cgccagcagt acaggcaggt
      841 ttttctcggc aggcgtggtg gagaaatgct tgtccatcgc gtgtgcgcca gaaagcagtt
      901 caacgaagtt atcaaagccg atggagagaa caatcgacag gccaatcgct gaccacaaag
      961 agtaacggcc gccgacccag tcccagaact cgaacatgtt ggcagtatca ataccaaact
     1021 cgccaacggc tttggcattg gttgagagcg ccgcaaagtg tttcgcaacg tgtttttcat
     1081 cacctgccgc tttcaggaac cagtcacgcg cgctatgggc gttggtcatg gtttcctgag
     1141 tggtgaaggt tttagatgct accaggaaca gcgtggtttc cgggtttact tttttcagca
     1201 cttccgcgat gtgagtccca tcgacgttag aaacaaagtg catgttcagg tgatttttgt
     1261 acggacgcag tgcttcggtc accatgtacg ggccgaggtc agaaccgccg atcccgatgt
     1321 tcactacgtc agtgattgct ttgccggtat aacccttcca ctcaccggag ataatcgctt
     1381 ccgagaaggt tttcatcttc tccagcaccg cgttgacttc cggcattacg tctttgccat
     1441 caaccaaaat cggggtattg ctacggttac gcagcgctac gtgcagcaca gcgcggtttt
     1501 cagtgcggtt gatcttctcg ccagagaaca tcgacttaat cgcgcccgcc agatcgcact
     1561 ctttcgccag atcttgtaat ttcgccagcg tctcttcagt gatgcggttt ttggagtaat
     1621 ccaccagcat ctgatcgccg aaggttgcgg agaacttaga aaaacgatcg ccgtctttag
     1681 caaaaagatc ggcgatcgta acgtctttca tttcatcgaa gtgtttctgt agtgcctgcc
     1741 aggcagcggt ctgcgttgga ttgatgtttt tcattagcaa tactcttctg attttgagaa
     1801 ttgtgacttt ggaagattgt agcgccagtc acagaaaaat gtgatggttt tagtgccgtt
     1861 agcgtaatgt tgagtgtaaa cccttagcgc agtgaagcat ttattagctg aactactgac
     1921 cgccaggagt ggatgaaaaa tccgcatgac cccgtcgttg acaaccgccc cgctcaccct
     1981 ttatttataa atgtactacc tgcgctagcg caggccagaa gaggcgcgtt gcccaagtaa
     2041 cggtgttgga ggagccagtc ctgtgataac acctgagggg gtgcatcgcc gaggtgattg
     2101 aacggctggc cacgttcatc atcggctaca ggggctgaat cccctgggtt gtcaccagaa
     2161 gcgttcgcag tcgggcgttt cgcaagtggt ggagcacttc tgggtgaaaa tagtagcgaa
     2221 gtatcgctct gcgcccaccc gtcttccgct cttcccttgt gccaaggctg aaaatggatc
     2281 ccctgacacg aggtagttat gtctgaaatt gttgtctcca aatttggcgg taccagcgta
     2341 gctgattttg acgccatgaa ccgcagcgct gatattgtgc tttctgatgc caacgtgcgt
     2401 ttagtagtcc tctcggcttc tgctggtatc actaatctgc tggtcgcttt agcggaaggg
     2461 ctggaacctg gcgagcgatt cgaaaaactc gacgctattc gcaacatcca gtttgccatt
     2521 ctggaacgtt tgcgttaccc gaacgttatc cgtgaagaga ttgaacgtct gctggagaac
     2581 attactgttc tggcagaagc ggcggcgctg gcaacgtctc cggcgctgac agatgagctg
     2641 gtcagccacg gcgagctgat gtcgaccctg ctgtttgtcg aaatcctgcg cgaacgcgat
     2701 gttcaggcac agtggtttga tgtacgtaaa gtgatgcgta ccaacgaccg atttggtcgt
     2761 gcagagccag atgtagccgc gctggcggaa ctggccgcgc tgcagctgct cccacgcctc
     2821 aatgacggct tagtgatcac ccagggattt atcggtagcg aaaataaagg tcgtacaacg
     2881 acgcttggcc gtggaggcag cgattatacg gcagccttgc tggcggaggc tttacacgca
     2941 tctcgtgttg atatctggac cgacgtcccg ggcatctaca ccaccgatcc acgcgtagtt
     3001 tccgcagcaa aacgcattga tgaaatcgcg tttgccgaag cggcagagat ggcaactttt
     3061 ggtgcaaaag tactgcatcc ggcaacgttg ctacccgcag tacgcagcga tatcccagtc
     3121 tttgtcggct ccagcaaaga cccacgcgca ggtggtacgc tggtgtgcaa taaaactgaa
     3181 aatccgccgc tgttccgcgc gctggcgctt cgtcgcaatc agactctgct cactttgcac
     3241 agcctgaata tgctgcattc tcgcggcttc ctcgcggaag ttttcggcat cctcgcgcgg
     3301 cataatattt cggtagactt aatcaccacg tcagaagtga gcgtggcatt aacccttgat
     3361 accaccggtt caacctccac tggcgatacg ttgctgacgc aatctctgct gatggagctt
     3421 tccgcactgt gccgggtgga agtagaagaa ggtctggcgc tggtcgcgtt gattggcaat
     3481 gacctgtcaa aagcctgcgg cgttggcaaa gaggtattcg gcgtactgga accgttcaac
     3541 attcgcatga tttgttacgg cgcatccagc cataacctgt gcttcctggt gcccggcgaa
     3601 gatgccgagc aggtggtgca gaagctgcat tttaatttgt ttgagtaata tttccctact
     3661 ggcgtataac gctcaccgat tgttatacgc catcctcact gaaaacatag attaaataat
     3721 atctgaaaag caaaatcact cctgtagaac agtattataa tcaccgaaaa atatcattta
     3781 acaggagtgt taatggctac tttaatggaa aaagatgcac ttttaaacgg agccagtcag
     3841 tgcatcacgt ttttatcaaa tatcattgat aattgctcag tttcgtcgca tcaggattca
     3901 ggcgatgctc tcaagcgatt agtcagctat cgcgattatc tttattctac ccccgctgaa
     3961 cttgttgatt ttacacaagg taagattctc cttcagcagg taagaactca atatcagcat
     4021 gaattcaaca atactacgca ttcagaaaat aaagcatctt ttgattctat ttggcagcgt
     4081 ctgacaaatc atgaagtcac accgcaacag catcccattg gttttgtttt aggtgggcaa
     4141 cccggagcag gtaaatcatc tctgattgaa ttagccaaaa gagagacaaa agataatata
     4201 atgattatta acggtgatga ttttcgattt ctacaccctg attttaatta tatctaccaa
     4261 aattatgggg atgattttgt tacccataca gcgaaatttt ccggcgaaac ggtcgaacgt
     4321 gcaattgaga gagcgatagt cagtaaactc aacattgttg tcgaaggaac atttcgtaat
     4381 gccgcgacgc cgttacaaac attaaaaaaa ctcaaagatg caggctacca aacagaagtc
     4441 atgataaaaa caacctcagc ggcattaagc tgggaaagca ccaatgaacg gtatagtaag
     4501 gacaaagagg cgggaaatat tgcccgaaaa gtggataaaa accaccatga tatcgtcacc
     4561 ggtttgctgg cagaaaatgc ccgcaaggtg tttgcttcga acctgtcaga taagtttgcg
     4621 gtgtattcgc gtgagaagat gatatttagt tcacaagcgg caacgaatga cgatattgca
     4681 acacttatac agaatgaaat cagcgggaat acacaataat aataagagct gatggcgtga
     4741 aataacgccg ggctttatgc ccggcgcatt accgtctggt acattaagct atccagttcc
     4801 ttaacgtccc cttctctgct atccggtaac agcggtggaa tttccccacg tgcctgttcc
     4861 gcacgaataa acgcatgtaa caatggacgg cgcggaccat attccgcttc tcctgcgcgc
     4921 tgtttacgtt ccagtaattc gtctatttct gcacgtaacg gcgcatcaag ttcgcttccc
     4981 gccagtaact cagcaaagcg cattggcggt acgcctttac ccgcttcgac ccagcgcacg
     5041 gccagcagcg gacgcagcac gtaaaagtat tttttcagcc gcacttcgtc gccttgtaaa
     5101 taaccgcgaa agtttttccg cgccatcgaa taataatgcc aacgcgcacg cagcggagaa
     5161 aaccacttcg gcaccattgc cttaagcgcc gttatcgttt cttcatcttg ttgatagacc
     5221 acaggagaat ccagccattc tatcagcgtc ggattcgcac ctttcaataa tcccagcgcc
     5281 ttgcgccact cccagcctga aacatctaac tcgtcgtcaa taggcaactc aataacatcg
     5341 cgcggcgcct ctactcgcag ataccattcc agcgggtgaa catacaaaaa ccgtacatcg
     5401 tagtcgctgt cgggcgaggc aaatccccag ccgcggctcc ctgattcaca ggcgtacagt
     5461 actttcacat cataccgccg ttcaatttct cttaactgct gcgataccct ttctcgcatc
     5521 gcagcactga ctccatttaa tggcatgtta ctcacccttt gatattaacc aacccatgca
     5581 gaacctgatc cagaccgcca aatacagaga tgttatcgat ccgttcagcc actctctcca
     5641 gcgtttccag ttcttttaag cgtaacgcta ccgggttgtt ttccatgact ttagcggtat
     5701 tgagtaacga acgcgtcgct gccgtttctt cacgtcggcg aatcacattg gcctgcgcgg
     5761 atttctccgc ctcaaccagc tgcgcaagga tatttttcat atcgccaggc aggacgatat
     5821 ctttcacccc taacgaggca atctccatac caaatggcaa catgcgcgat ttcacctgtt
     5881 cgctgacaac gtcatcaatg acctgtttat cttccagcaa ttcatccaac gtacgcgtac
     5941 ctaccgcttc acgcagggca aattgtaatt cccgatacaa atgatcaatc ggcttcgtta
     6001 actggctaaa tgccaataac acgtcgctgt agcgccagtt agcggcgagg ttaatccgca
     6061 gattgacctt atctttcgtt aagatttctt gcccgctgac ttccagtacc tgcaagcggg
     6121 tgtcaaccac ttcagcgtca accagatggt taattttcca gtatgcggtc agccccggcg
     6181 gtaacagcgc ctgcgtttca ccatcgattt tcagcacgcc tacatgccag gccggaacct
     6241 gcaccgttag tattgcatcg cggcctttaa ctgccccttt gcgccgtggt tgcaataccg
     6301 cattcatcac ctccgtttgt acctggactt gctgcgtatt catccgcacc agtgtcaaat
     6361 catcctctac acgccagtac aaacggcgcg ttgatggcgg caggatctcc tgcaaaatac
     6421 cgtccatata caacgccccg gcttcagtct ccgacaagtc ggccaccacg caatatcttt
     6481 ccacccaatc aggctgaaag cggcgcaaat aatccgccag cacgtcgggc acttcgctac
     6541 catccagcgt aatcagcaac acttccgggg tagtaaacca gggtaaaata tgctcgcctg
     6601 catgtagcac tttgtagtaa tcgccgtttc tggagagtaa tgccagctga tcttttctta
     6661 cgcttatttt cttaatcatt atctattcct tgcttaacag ccgtcgcgga agcggacacg
     6721 aagcgacagg ggaattccct tttgcctgtt acccggcgtc tttatcggcg acgttatcgg
     6781 ggatcgctga ctgcgcaatc cctcattttt tatttaataa gttacaacca gtattcattt
     6841 caggaatgaa tgtgggaatc gaacccataa ttggcgtcaa aggctgacgg aacacgcccg
     6901 gaaaacagcc tgaccgttcc tggtgtgccg acggggttta accccctttg aaattcagta
     6961 tcaacgcaaa agtgttattg cgagaagcgt gccagaattg agaaaaattc ttaaccaaat
     7021 gaaaaataaa gagttagtga attacttttg tttgtagggg gaaaactttt cgctaaatgt
     7081 gtatctgtat ttatcgtcaa ttatcaagaa agataaatat ttaagaagag aacaaaggaa
     7141 tattatcagt tctgcttgca aatgaattcc cgtacactga atccatcggc taactgaggg
     7201 caacatcatg gcactcccgc gtattaccca aaaagagatg acagagcgcg agcaacgcga
     7261 gctaaaaacg ctgctggacc acgcgcggat tgcgcatggt cgcgtactga ccaactccga
     7321 aaccaacagc attaaaaaag agtacatcga taagctgatg gttgagcgtg aagcagctgc
     7381 aaaaaaagcc cgccagttga agaaaaagca ggcttataaa ccagatactg aagcatcgtt
     7441 taactggtcg gctaatacac caacgcacgg caggcgctga ttaacgcccc ttctttttac
     7501 gccccggcga tgtgaaacgt ttaccatttg acgccgggcg gccacctttt tctgatgttt
     7561 tttccatctt caccaacggg cgtttgatgc ccgccgtttt cggtttggcc ttcactttag
     7621 gttttgcctc ggaagaggaa ttttcgatga gcttaaacag gtcgattaac tcatcgtcag
     7681 ttaaatcgcg ccactcaccc agcggaatgc cgcttaagct gacgttcata atgcgcgtac
     7741 gttccagctt tttcacttca tagccgaaat gctcgcacat gcgccggatc tgacggttca
     7801 gcccctgcac cagggtaatg cggaagacaa acggcgcttc ttttttgact ttgcactttt
     7861 tcgtcaccgt cccgaggatc ggcacccccg cccccatacc acgaataaat tcatcggtaa
     7921 tcggtttatc gaccgtcacc agatactctt tctcatgatc gttacccgca cggaggatct
     7981 tattcaccag atcgccgtga ttggtgagga aaatcagccc ctgggagtct ttatccaggc
     8041 ggccaatcgg gaacacgcgt ttgctgtggt taacgaaatc gacaatgtta tcgcgctcgc
     8101 catcttcggt ggtgctgaca ataccgacgg gcttgttcag ggcgataagt accaaatctt
     8161 cggcttcccg aggctcaatc aactgaccgt ttactttcac aacgtcgccg ggtttcacct
     8221 gatcgccaat ggtggctcgc ttgccattaa ggaacacatt gccttgctcg atatagcgat
     8281 ccgcttcgcg gcgtgagcaa attccgcttt cgctgatgta tttatttaaa cggactgatg
     8341 agtcgggcag cataaattct cctgtaaaaa acggaatata ccgtaccttt gggttgataa
     8401 aaaatagtca tgagcgaact atttgttatt tccattggct tgaattcgtt acctgctcaa
     8461 atcactttcc agaagcaatg tgcgacatcg tgagccatgt cagtaagtgg ttaaattcgc
     8521 agtggtagca tgcctcccgt gatttcttac taaccggaga agtaatggaa ctgcttttat
     8581 tgagtaactc gacgctgccg ggtaaagcct ggctggaaca tgcactgccg ctaattgctg
     8641 aacagttgca gggtcgccgc tcagcggtgt ttatcccttt cgctggcgta acgcagacct
     8701 gggatgatta cacagcgaaa acggctgcgg ttctcgctcc gctgggtgtt tctgtcaccg
     8761 gtattcatag cgttgtcgat cccgttgccg cgattgaaaa tgctgagatc gtgattgtcg
     8821 gcggcgggaa tactttccag ttgctgaaac agtgccgcga gcgcgggctg ctggcaccaa
     8881 ttactgacgt ggttaaacgt ggcgctctgt atattggctg gagcgcaggc gctaaccttg
     8941 cttgcccaac cattcgtacc accaacgata tgccgattgt cgatccgcaa ggtttcgatg
     9001 cgctaaatct gttcccgttg caaatcaacc cgcacttcac caacgcgctg ccggaaggcc
     9061 ataaaggtga aacccgtgag cagcgtattc gcgaactgct ggtcgtcgcg ccagaactga
     9121 cgattattgg tctaccggaa ggtaactgga tcacagtgag taaaggtcac gctacgctgg
     9181 gtggcccgaa caccacttat gtgtttaagg ctggtgaaga agcggtgccg ctagaagctg
     9241 gtcaccgttt ttaagtgaat tgccggatgc atgatccggc atcttgttca tagtgcctga
     9301 tgcgacgctt gacgcgtctt atcaggccta caggattcac aatattaata ctcgtcccgt
     9361 ccttcatctt catccggctg ttccagcaca ctgtaagcca ccgaacaaaa cagcgagttc
     9421 aggcgctgca tatctcccag taagcctaaa tgtagcgaac tggtttcaat gctctgcacg
     9481 ttttgctgat gcaggcgatc gacgtgggcg tgggaatagc ggcgattaag aatgcgaaag
     9541 cgatgcttac tacggcgcaa acgacgagcg ctggtgacat cgccagagaa gaacaccgac
     9601 attgccagtt ttaaattact gagcaattgc tcatagagcg catccagttc cttcaagcca
     9661 tcaagcgaaa atgcccgccg tgctgccagc gatttatcgg caatttcgcc gcccatgcgc
     9721 tcgacgatat cggaggcctg ttcaaggttg agcgacattt cgatgatctc cgcccagcgg
     9781 cgcgactctt cctccgccaa ctcttctttt ggcatccgcg ccagatacag cttaatggcg
     9841 gtgtagagaa cgttgatatc atctgccagc ttacgcagct ctttctcctg ccgtggctcg
     9901 ccgtgcatca ctttatttag cccttccatc atctgttcca tggcgtcgcc aatgcgcagg
     9961 gtttcgcgcg cggcgttcgc cagagcaagc gtgggcgtat ccagcgcgct gacatccaga
    10021 tgtttgggcc gtagctgggt atccagttcc ggttcatcgc gaataatcgt tttgcaaaac
    10081 cgtgccatcg ggtcaacaaa tggcagcatg accaggcaac gtacaaggtt gtagaagacg
    10141 tggaaataga tcaccagttc cgcttttggc aatgacaact tccccattgt ctctgccagc
    10201 aaatggacaa acggcaggat aatcaggcta cccaccagct taaacagcag actacccagc
    10261 gcgacacggc gggctgcggc attggcggca ctgttgttga gcatcgccag cagaccggaa
    10321 ccgaggttag caccaatcac cagacagagc gccacgggga aggagataat gcctgcggcg
    10381 gtcagagtcg cggtcagcag tacagcagca aggctggagt agctgataat ggcgaacatc
    10441 gcgccaatca gcgcatccag cagaatatcg ccggtcagcg aggcaaagat cacctgaacg
    10501 ccgtttgcct gggtgatcgg cgttacggcc tgcacaatca actccagcgc cagcaaaatc
    10561 agcccaagac caataccgac gcggcccagt tgcccggcgc gtgactgttt gcgtccgagg
    10621 aaaaaaatca cgccgataaa aataagtaac ggtgacagcc aggataagtc gaaggtgaga
    10681 atacgcgcca ttagcgccgt cccgacatct gcacccagca caatgaccag agccggtgcg
    10741 agggctacca gatcctgagc gacaaacgag gtcaccagca tggtggtggc attactgctc
    10801 tgtaccagtg cggtaacgcc gatccccgcg caaaaggcga gcggcttttt ttcgacgctc
    10861 cggctaagga cagtacgcaa acgcgcgccg aagacgcgca ttacgccggt tcgaacaata
    10921 tgagtccccc agaccagcag ggcgacggca gaaagcaggt gaagcagcgt taacacgagt
    10981 tcaggttctc cttatcgtta tatgttcctg aagcaattga atgaccaaag gaccggaaac
    11041 acgctgactg gcatctttac tgtgcctttt gaccttcgtt tttccggcta agattattcc
    11101 agcctgattc cagtataagt gtttcatatt aataaagaga cagggcactc attgagcgcc
    11161 ctgtttgttg taaggaaaag tgacagattt gtgaatcagt ccgcgtcata ccccagattc
    11221 ggtgccagcc agcgctcaac ttcggtaacg ctcatacctt tacggcgggc ataatcttca
    11281 acctgatcgc gctgaatttg tgctacagcg tagtacttgc tgtccgggtg gctgaagtac
    11341 caacccgaaa ccgatgcacc gggccacatg gcgaaagatt ctgtgagttt catgccagtg
    11401 tgtttttcca cttccagcag ctcccagatg gtggcttttt ccgtatgttc cgggcaggcc
    11461 ggatagcccg gtgccggacg gatgccctgg tagttttcgc ggatcagttc ttcgttgctg
    11521 aggttctcgt tcggcgcata gccccagtag actttacgca cacgctcatg gagatactcc
    11581 gcaaaggctt cggctaaacg gtcggcaagc gctttcacca tgattttgtt gtaatcatcg
    11641 tgctgcgctt caaaggcatc agccagtgcg tcctcttcca gcccgccagt cacggcaaat
    11701 gcgccgatgt aatctgcttt accagaaagc ttcggcgcaa cgaagtcagc gagacagtag
    11761 ttagcgaatc ccgttttttc ggtctgttga cgcagatggt ggctgacgtt gatcacatgg
    11821 gtgcgcgttt cgtcacggta gatttcaatg tcatcgccca cacggtttgc cgggaacagg
    11881 cccaccacgc cacgcggatt cagcgttttc tcggcgctta atttatccag catgtcgttg
    11941 gcgtctttaa acagccgctg cgcctcaacg cccaccactt catcttccag aatgcgcgga
    12001 tacttcccgg ccagcgacca ggtcataaag aacggtgtcc agtcgatgta attacgcaga
    12061 gtttcgatgc tggcttcaac ttcctgcacg ccgagacggt gcgccaccgg cggcgtgtaa
    12121 gcctgccagt caaaagcgaa atcgttatcg cgcgccgctt ccagcgtgac cggtggtgtg
    12181 cgcggtttct tacgcccgtg ctgaatacgt acggtttcgt actccttgcg ggtacgagcg
    12241 acaaaatcat cacgctgagt atcggaaagc agcgccgcca ccacaccaac ggtgcgcgat
    12301 gcgttctgta catacaccgt cgggccgctg tagttctgct caattttcac cgccgtgtgc
    12361 gcttttgagg tcgtcgcgcc gccaatcaat agcgggatgg tgaagccctg acgctccatc
    12421 tctttcgcca cgttaaccat ctcgtccagc gacggcgtga taagccccga aagtccaatc
    12481 agatcagcat tcacttcttt agcggtacgg agaatttttt ccgcaggcac cataacgccg
    12541 agatcgacaa tttcgtagtt gttacattgc agcaccacgc caacgatatt tttaccgatg
    12601 tcgtggacat cgcctttcac ggtggcgatc accatcttgc cgttggtttt accctgctct
    12661 ttgctggctt caataaacgg ttcgagatac gccactgcct gtttcatgac gcgcgccgat
    12721 ttgaccacct gtggcaggaa cattttccct tcgccaaaca gatcgccgac cacgttcata
    12781 ccgtccatca gcgggccttc aatcacttca atcgggcgcg tagcctgctg gcgggcttct
    12841 tcggtatcct gctcgataaa ctcggtaatg cctttaacca gcgagtattc cagacgttta
    12901 ttcacttccc acgagcgcca ctccgcctgc tgggcgttag cggtgtcgtc ggttttgctg
    12961 ccgcgatatt tctcggcaag ctccagtaaa cgctcagtgc catcgtcgcg acggttaaga
    13021 atcacatctt ccaccgcgtc gcgcagttca gcgggtaggt cgtcgtaaat cgccagttgc
    13081 ccggcgttga cgatccccat atccatgcca ttgcgaatag cgtagtagag gaacaccgcg
    13141 tgaatggctt cgcgcaccgg atcgttgcca cggaatgaga aagaaacgtt agatacaccg
    13201 ccggaaatca gcgcgtgcgg cagttcgcgt ttgatatctt cacacgcgcc gataaagtcc
    13261 tgcgcgtagt tgttgtgctc ttcaatgccg gttgcgaccg cgaagatgtt cgggtcgaag
    13321 atgatatctt ctggcgggaa gccaacctct tcggtgagga ttttgtacgc ccgacggcaa
    13381 atctcgattt tccgtgcgcg ggtatcggcc tgtccctgtt cgtcaaaggc cattaccacc
    13441 actgccgcac cgtaacgacg caagagcttc gcgtgatgga taaaggcgtc gacgccctct
    13501 ttcatcgaga tagagttaac aatgcctttg ccctggatac acttcagacc tttttcaatg
    13561 acatcccatt ttgaggagtc gatcataatc ggcacgcgag cgatatccgg ttcaccggca
    13621 atcagattga gaaaacgcac catcgccgct tcggcgtcga gcatcccttc atccatgttg
    13681 atatcgataa tctgcgcacc gttttccacc tgctggcggg ccacatccag tgcctcgctg
    13741 tatttctctt ctttgatcag gcgcttgaac ttagcggaac cggtgacgtt ggtgcgttca
    13801 cccacgttca caaacaggct atcttcgcca atgttcagcg gctccaggcc ggacaaacgg
    13861 caggctacgg ggatttccgg cagtttgcgc ggcgctaatc cttctactgc acgactcatc
    13921 gctgcaatat gttgtggcgt ggtgccacag cagccgccga cgatattgag aaaacccgct
    13981 tgcgcccatt cacgtatctg ttttgccatc gtgtcggcgt cgagatcgta ctcaccaaag
    14041 gcgttgggta gcccggcgtt cgggtgcgcg gtgacgtagc attccgcaat ccgtgacagc
    14101 tcctgcacgt actggcgcag ttcatcgggc cccagcgcac agttcaggcc aaaggtcaga
    14161 gcttcggcgt ggcgcaatga gttgtaaaat gcttcggtgg tctgcccgga gagcgtgcgc
    14221 ccggaggcgt cggtgatggt gccggagatc ataatcggca gctcaacgcc cagcgcttca
    14281 aactccgttt tcaccgcaaa aaccgccgct ttggcgttaa gggtgtcgaa aacggtttca
    14341 atcaggatca gatccgcgcc accttccacc agcgctttgg tggactctcg ataagccgcc
    14401 accagcccgt caaaagtgat attacgaaat gccggatcat ttacgtccgg agaaatagac
    14461 gccgtgcggt tggtcgggcc gagaacaccg gcaacgtagc gcggtttctc cggcgtgcgc
    14521 gcggtccact catcagcaca agctcgcgcc agtttcgccg ccgcaaagtt gatttccgcc
    14581 gacagggatt ccatctggta atccgccatc gcaatggtcg tggagttgaa ggtgttggtt
    14641 tcgatgatat ccgcgcccgc ttcaaagtag gcgttgtgga tagcggcgat cacttccggt
    14701 ttgctgagca ccagcaggtc gttgttgcct ttgagatcgc atggccagtc ggcaaagcgt
    14761 tcaccacgaa aatcggcttc gttcagtcga tagctctgga tcatggtgcc cataccgccg
    14821 tccagcacca gaatacgttg atttaactgc gcacgcagtt gttccacttt gctgctcaca
    14881 cttgctcccg acacgctcaa cccagattta ataaaaattc aacaaaccat actggcataa
    14941 acgcatctgt ggtaaaagcg accaccacgc aacatgagat ttgttcaaca ttaactcatc
    15001 ggatcagttc agcaactatt gcattagcta acaataaaaa tgaaaatgat ttccacgata
    15061 cagaaaaagg aggctgtcat ggtcgcaccc attcccgcga aacgcggcag aaaacccgcc
    15121 gttgccaccg caccagcgac tggacaggtt cagtctttaa cgcgtggcct gaaattactg
    15181 gagtggattg ccgaatccaa tggcagtgtg gcactcacag agctggcgca acaagccggg
    15241 ttacccaatt ccacgaccca ccgcctgcta accacgatgc aacagcaggg tttcgtgcgt
    15301 caggtcggcg aactgggaca ttgggcaatc ggcgcacatg cctttatggt cggcagcagc
    15361 tttctccaga gccgtaattt gttagcgatt gttcacccta tcctgcgcaa tttaatggaa
    15421 gagtctggcg aaacggtcaa tatggcggtg cttgatcaaa gcgatcacga agcgattatt
    15481 atcgaccagg tacagtgtac gcatctgatg cgaatgtccg cgcctatcgg cggtaaattg
    15541 ccgatgcacg cttccggtgc gggtaaagcc tttttagccc aactgagcga agaacaggtg
    15601 acgaagctgc tgcaccgcaa agggttacat gcctataccc acgcaacgct ggtgtctcct
    15661 gtacatttaa aagaagatct cgcccaaacg cgcaaacggg gttattcatt tgacgatgag
    15721 gaacatgcac tggggctacg ttgccttgca gcgtgtattt tcgatgagca ccgcgaaccg
    15781 tttgccgcaa tttcaatttc cggaccgatt tcacgcatta ccgatgacag cgtgaccgag
    15841 tttggcgcga tggtgattaa agcggcgaag gaagtgacgc tggcgtacgg tggaatgcgc
    15901 tgactttttc tggcgggcag aggcaatatt ctgcccatca tacctgagtg gcaatagaat
    15961 aagggtgtct gttaatcgca ttgacgccaa aataacttaa tgtcatacac ttcacacact
    16021 tttattaact cagcattatt tttaaacatc aaaccactta atcattaaca aaatcgcaag
    16081 atctcattcc accgaaactt ttacggctgc attatgagat t
//
LOCUS       DAZFXT010000106        15843 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.106, whole genome shotgun sequence.
ACCESSION   DAZFXT010000106 DAZFXT010000000
VERSION     DAZFXT010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.106"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(26..751)
                     /gene="phoU"
                     /locus_tag="ACHKW0_003627"
     CDS             complement(26..751)
                     /gene="phoU"
                     /locus_tag="ACHKW0_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HFO7476393.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(766..1539)
                     /gene="pstB"
                     /locus_tag="ACHKW0_003628"
     CDS             complement(766..1539)
                     /gene="pstB"
                     /locus_tag="ACHKW0_003628"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HFO7476394.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(1630..2520)
                     /gene="pstA"
                     /locus_tag="ACHKW0_003629"
     CDS             complement(1630..2520)
                     /gene="pstA"
                     /locus_tag="ACHKW0_003629"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HFO7476395.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(2520..3479)
                     /gene="pstC"
                     /locus_tag="ACHKW0_003630"
     CDS             complement(2520..3479)
                     /gene="pstC"
                     /locus_tag="ACHKW0_003630"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HFO7476396.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(3565..4605)
                     /gene="pstS"
                     /locus_tag="ACHKW0_003631"
     CDS             complement(3565..4605)
                     /gene="pstS"
                     /locus_tag="ACHKW0_003631"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HFO7476397.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(4919..6748)
                     /gene="glmS"
                     /locus_tag="ACHKW0_003632"
     CDS             complement(4919..6748)
                     /gene="glmS"
                     /locus_tag="ACHKW0_003632"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HFO7476398.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(6910..8280)
                     /gene="glmU"
                     /locus_tag="ACHKW0_003633"
     CDS             complement(6910..8280)
                     /gene="glmU"
                     /locus_tag="ACHKW0_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HFO7476399.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(8631..9050)
                     /locus_tag="ACHKW0_003634"
     CDS             complement(8631..9050)
                     /locus_tag="ACHKW0_003634"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HFO7476400.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(9071..10453)
                     /gene="atpD"
                     /locus_tag="ACHKW0_003635"
     CDS             complement(9071..10453)
                     /gene="atpD"
                     /locus_tag="ACHKW0_003635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HFO7476401.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(10480..11343)
                     /gene="atpG"
                     /locus_tag="ACHKW0_003636"
     CDS             complement(10480..11343)
                     /gene="atpG"
                     /locus_tag="ACHKW0_003636"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HFO7476402.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(11394..12935)
                     /gene="atpA"
                     /locus_tag="ACHKW0_003637"
     CDS             complement(11394..12935)
                     /gene="atpA"
                     /locus_tag="ACHKW0_003637"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HFO7476403.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(12948..13481)
                     /gene="atpH"
                     /locus_tag="ACHKW0_003638"
     CDS             complement(12948..13481)
                     /gene="atpH"
                     /locus_tag="ACHKW0_003638"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HFO7476404.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(13496..13966)
                     /gene="atpF"
                     /locus_tag="ACHKW0_003639"
     CDS             complement(13496..13966)
                     /gene="atpF"
                     /locus_tag="ACHKW0_003639"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HFO7476405.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(14028..14267)
                     /gene="atpE"
                     /locus_tag="ACHKW0_003640"
     CDS             complement(14028..14267)
                     /gene="atpE"
                     /locus_tag="ACHKW0_003640"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HFO7476406.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(14314..15129)
                     /gene="atpB"
                     /locus_tag="ACHKW0_003641"
     CDS             complement(14314..15129)
                     /gene="atpB"
                     /locus_tag="ACHKW0_003641"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HFO7476407.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(15138..15518)
                     /gene="atpI"
                     /locus_tag="ACHKW0_003642"
     CDS             complement(15138..15518)
                     /gene="atpI"
                     /locus_tag="ACHKW0_003642"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HFO7476408.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         3794 a         4322 c         3962 g         3765 t
ORIGIN      
        1 aagttattgg gatttgtctg gtgaattatt tgtcgctatc tttccccgcc agcagtttat
       61 ccagctcatc gccaccgacg tgacggaaat cctgcccctt cacgtagtag aagataaact
      121 cacaaatatt ctggcagcgg tcgccaatac gttcgataga acgcgcgcag aacagcgcag
      181 taagtacgct cggaatggta cgcgaatctt ccatcatgta ggtcatcagt tgacgaacaa
      241 taccttcgta ttcctgatcg acttttttat cttcacgata aatacgtacc gcttcgtcaa
      301 tgtccatccg cgcgaacgcg tccagcacgt cgtgcagcat ctggatggta tgacggccca
      361 gcgactccag acttaccagc aacggctgat gctgctggga gaatttctcc agcgcagtac
      421 ggcagatttt gtccgccacg tcgccaatac gctccagctc ggcaatggtt ttactgatca
      481 ccataaccag tcgcaggtcg ctcgccgtcg gctgacgttt ggcgataatg cgcacgcagg
      541 cttcatcgat cgccacttcc atcatgttga cgttcttgtc gccttcgatc acgcgcttcg
      601 ccagatcgct gtcctggtta tgcatcgcgg tgattgcatc agaaagctgc tgctccacca
      661 tgccgcccat ggtcatcacc tgcgtgcgga tactttccag ttcggcgttg aactggccgg
      721 aaatatgttt attaagattg agactgtcca taacgcactc ctgaatcaac cgtaacgacc
      781 ggtgatgtag tcttctgttt gtttcttcgc tggcttggtg aacagatcgt ccgtgttgct
      841 gaactcaatc aattcgccca ggtacataaa cgccgtgtgg tcggaacaac gcgcagcctg
      901 ctgcatgttg tgggtgacga tcaccacggt gtaatcctgc ttcagttcgg tgatcagctc
      961 ttcaatacgc ccggtagaga tagggtcgag cgccgaacac ggttcgtcga gcagcagcac
     1021 ttccgggcga atggcgatac cacgcgcaat acacagacgc tgttgctgac caccagagag
     1081 agagtaaccg ctctggtgca atttatcttt ggtttcgttc cacaatgcgg ctttggtcaa
     1141 tgcccactgc acgcgctcgt ccatgtcggc acgggagagc ttctcaaaca gacgaacgcc
     1201 aaaagcgatg ttgtcgtaga tggacatcgg aaacggcgtc ggtttctgga acaccatgcc
     1261 cactttcgca cgcagcaggg cgatatcctg agagttggtc aggatgttgt cgccatcaag
     1321 cagaatttca ccttctgcgc gctgctccgg atacagttca aacattttgt tgaaggtacg
     1381 cagcagcgtc gatttaccgc agccggacgg cccgataaac gccgttacct ggtttttagc
     1441 gatatccagg ttgatgtttt tcagggcatg gaatttgccg tagtagaagt tcaaattacg
     1501 aacctgaatt ttactcgggg cagtttcaac catactcatt gcaatctttt cctcatcgta
     1561 cctgatgctt ttcgctaatc aggcttaccg taggccgggt aaggcgttca cgccgcatcc
     1621 ggcaaagtgt taaccgtgtt tattcttcgc aaaaacaacg cgcgccagaa tgttcagcag
     1681 caggacgcac agggtaatga tcaatacccc ggcccaggcc agttgttgcc attccgcaaa
     1741 cgggctcatc gcaaacttaa agatcgtcac cggcaggttg gcgatcggct gcatcatgtc
     1801 cgtgctccag aactggttgg agagcgcggt aaacagcagc ggcgcggttt caccggcaat
     1861 acgggcaatc gccagcagga taccggtcat aatcccggac accgacgctt tcagcgtaat
     1921 cgcggagatc atcttccact tcggtgtacc cagcgcataa gccgcttcac gcaggctgtc
     1981 cggcaccagt ttcagcatgt tctcggtggt gcggataacg atcggcacct gcaacagcgc
     2041 cagcgcaatt acgcccgccc agccggagaa gtgctccatc tgtgccacca cgatggtgta
     2101 gacaaacaga ccaaccacaa tcgacggcgc ggagagcaga atgtcgttaa tgaagcgaat
     2161 cacttctgcc agccaggatt tacgaccata ttccgccaga taaatccccg ccataatgcc
     2221 cagcggcgta ccgaataccg tggcccacaa aattaacagc ccgctacccg ccagggcgtt
     2281 cgccagacca ccaccttccg tattgggcgg cggcgtcatt tcagtgaaca gcgccagcga
     2341 cataccgtcg ataccgcgag tgatggtgga cattaaaatc cagatcagcc agaacaggcc
     2401 gaaggccatc gtcgccatcg agagcgtcag cgcaatacgg tttttgaggc ggcgacgcgc
     2461 ctgcattttg cggcgagatt cagccagcgc cgcagtggtt tgcatttcaa ccatagccat
     2521 tagcgtgccc cctcattctt agccaggcgc ataatcataa acttcgatgc ggcgaggacg
     2581 atgaaggtaa tcacaaacag gatcaggccc agttccatca gtgcggcaac gtgcagaccg
     2641 gattccgctt ccgcaaattc gttcgccagc gcagaggtga tactgttgcc cggcatatac
     2701 agcgaggcgc tgtcgagctg gtaggtgtta ccgatgataa aggtcaccgc catggtttca
     2761 ccgagcgcgc ggcccagccc cagcatgatg ccgccgataa caccattttt ggtgaacgga
     2821 agaacgatac gccagataac ttcccaggtg gtgcagccaa taccgtaggc cgactctttc
     2881 atcatcaccg gggtttgttc gaacacatca cgcattaccg ccgcaatgta cggaataatc
     2941 atgatggcga ggatcacgcc tgccgcgagg ataccgatac caaatgcggg gccagagaac
     3001 agcgcgccaa caatcgggat attcgacatg atattgccga ccggctcctg gaagtaaacg
     3061 gcgaatagcg gcgcaaagat aaacaggccc cacatgccgt aaacgatact tggaatggct
     3121 gccagcagct caatggcgat acccagcggg cgtttcagcc agccaggcgc aagctcagtc
     3181 aggaacaggg cgataccgaa actcaccggg acggcgatca gcagcgcgat aaacgaagtc
     3241 accagcgtac cgtagatcgg cactagcgcc ccgtagatat cgttcggtgc atcccactct
     3301 ttggtccaga ggaaagccag accaaatttc tgaatgctcg gccaggagga gatgatcaga
     3361 gagacaataa tgccacccaa catcaatagc acaatcagcg ccgccagttt taccagcacg
     3421 ctgaaaatta tgtcgccctt tttacccggt gggttaaaag caggcttggt tgcagccata
     3481 agttactctt cagttaaacg cgtttacaaa gtcattttgt aatgccggat aagccgttaa
     3541 tgccttatcc ggcctggagt tttattagta cagcggctta ccgctactgt ctttaatatt
     3601 ggtcttccac gcagcgcgaa cctgttcaac tacactatcc ggcaggctgg cgtaatccag
     3661 gtcgttcgcc tgtttagcac cggttttgta cgcccagtcg aagaatttca gcacttctgt
     3721 gccttgttct ggtttcttct gatctttgtg gatcagaatg aacgtggtag aggtaatagg
     3781 ccacgcatct tcgcctttct ggttggtcag atcctgagcg aaggttttgc tccagtctgc
     3841 gccttttgct gcattagcga agttttcttc ggtcggacta accggtttac catcagcgga
     3901 gatcagtttg gtatacgcca ggttgttctg cttcgcgtaa gcatattcaa cgtaaccaat
     3961 tgcacccggc agacgctgaa cgaacgcggc gataccgtca ttacctttac cgcccagacc
     4021 gatcggccat tttacggtag agccagtacc aacgttgttt ttccactctt cgttcacttt
     4081 cgccagatag ctggtgaaga cgaaggaggt accggagcca tctgcgcggc gtaccacggc
     4141 gatgttctgt gacggcagtt tcagacccgg attcagtttg gcgatggctt catcatccca
     4201 cttcttgatt ttgccgaggt agatgtcgcc gagggtttta ccatccagca ccagttcgcc
     4261 agacttcagc cctggaatgt taacagccag caccacgccg ccaatcacgg tcgggaactg
     4321 gaacagacct tcctgagcca gtttttcgtc agacagcggc gcgtcagagg caccaaaatc
     4381 aacggtatta gcgataatct gttttacgcc accggaagaa ccgataccct ggtagttaac
     4441 tttattaccg gtttctttct ggtaagtgtc agcccatttg gcatacaccg gcgcagggaa
     4501 ggttgcacct gcacctgtca ggcttgcttc tgcaaacaca gagaaagcgc tcatcgataa
     4561 ggtcgcggcg acaacagttg cgacggtggt acgcataact ttcataatgt ctcctgggag
     4621 gattcataaa gcattgtttg ttggctacga gaagcaaaat aggacaaaca ggtgacagtt
     4681 atatgtaagg aatatgacag ttttatgaca gagagataaa gtcttcagtc tgatttaaat
     4741 aagcgttgat attcagtcaa ttacaaacat taataacgaa gagatgacag aaaaattttc
     4801 attctgtgac agagaaaaag tagccgaaga tgacggtttg tcacatggag ttggcaggat
     4861 gtttgattaa aaacataaca ggaagaaaaa tgccccgctt acgcagggca tccatttatt
     4921 actcaaccgt aaccgatttt gccaggttac gcggctggtc aacgtcggtg cctttgatca
     4981 gcgcgacatg gtaagccagc agctgcagcg gaacggtgta gaagatcggt gcaatcacct
     5041 cttccacatg cggcatctcg atgatgtgca tgttatcgct acttacaaaa cccgcatcct
     5101 gatcggcgaa gacatacaac tgaccgccac gcgcgcgaac ttcttcaatg ttggatttca
     5161 gtttttccaa cagttcgttg ttcggtgcaa cgacgataac cggcatatcc gcgtcaatca
     5221 gcgccagcgg gccgtgtttc agctcgcctg cagcgtaggc ttcagcgtga atgtaagaga
     5281 tctctttcag tttcaatgcg ccttccagcg cgattgggta ctgatcgcca cggcccagga
     5341 acagcgcgtg atgtttgtca gagaaatctt ctgccagagc ttcaatgcgt ttgtcctgag
     5401 acagcatctg ctcaatacgg ctcggcagcg cctgcagacc atgcacgatg tcatgttcaa
     5461 tggaggcatc cagacctttc aggcgagaca gcttcgccac cagcatcaac agcacagtta
     5521 actgagtggt gaatgcttta gtggatgcca cgccgatttc cgtacccgcg ttggtcatca
     5581 gcgccagatc ggattcgcgc accagagaag aacccggaac gttacagatt gccagcgaac
     5641 caaggtaacc cagctctttc gacagacgca ggcccgccag ggtatccgcg gtttcgccag
     5701 actgtgacaa ggtgatcatc aggctgttac gacgcacggc agatttgcga tagcggaatt
     5761 cagaggcgat ttcgacgtcg cacggaatac ctgccagcga ttcaaaccag taacgggaaa
     5821 ccataccgga gttataagaa gtaccacagg cgaggatctg aatatgctca accttcgaca
     5881 gcagttcgtc ggcgttcggt cccagctcgc ttaaatcaac ctgaccgtgg ctgatgcgcc
     5941 cggtaagggt gtttttgatc gcgttcggct gttcgtagat ctctttctgc atgtagtgac
     6001 ggtaaatacc tttatcgccc gcgtcatatt gcagattgga ttcgatatcc tgacgtttta
     6061 cttccgcgcc agttttatcg aagatgttta ccgaacggcg agtaatttcc gcaatatcgc
     6121 cctcttcaag gaagataaag cgacgggtca ccggcaacag cgccagctgg tcagaagcga
     6181 taaagttttc gcccataccc aggccaatca ccagcggact accagaacgt gccgccagca
     6241 gggtatccgg gtgacgggag tccatgatca ctgtaccgta cgcaccacgc agttgcggga
     6301 tagcacgcag aacagcctca cgcagagtac cgccctgttt cagctcccag ttcaccagat
     6361 gggcaattac ttcggtgtct gtttcagaaa cgaaggtata gccacgcgct tttaactctt
     6421 cgcgcagcgg ttcatggttt tcgatgatgc cgttatgcac caccacaatg tgttcagaaa
     6481 catgcggatg cgcattcact tctgaaggtt caccgtgggt cgcccagcga gtatgagcaa
     6541 tacccgtacc gccatgcaga ggatgttctt ccgctgcctg agccagcatc tggactttac
     6601 cgaggcgacg caggcgggtc atatgacctt ccgcatcaac aacggccaga ccggcagagt
     6661 catatccgcg gtattccaga cgacgtaaac cttcaagaag gatttctgct acatcacgtt
     6721 gcgcgatcgc gccaacaatt ccacacatag tttttgattc cgatttatat cgttgtcggt
     6781 caacctgtat gcccgttttt tcgggcgccc cgagccttgt agagagtggg gttattttta
     6841 tagttactgc ttgtgggagg gagattatgt tatctcctca tcccatgtga ccggattacc
     6901 cggccagaat cactttttct ttaccggacg acgccagcct tctttctgag tctgcggcac
     6961 acggctgata gctaatgcat tttcgccgac attacgcgtc acagttgtac ccgcagcaat
     7021 ggtcgcgcct ttgcctactg ttaccggggc caccagctga gtgtcggaac cgacaaacac
     7081 atcgtcgccg ataatggtct taaatttatt cgcaccatcg tagttgcagg taatggttcc
     7141 cgcgccgatg ttaacgttat cgccaatttc cgcatcgccc aggtaagtca gatgaccagc
     7201 tttcgagcct ttacccagac gcgctttttt catctcaaca aagttaccga cgtgtgcacc
     7261 ttccagcaac tcagcaccag gacgcagtcg ggcaaacggg ccaatagtac aggccgctgc
     7321 cagattcgcg tcttccacaa cggtatacgg actgatttcg caatcatcgc caatcacgct
     7381 gtttttaatc acgcaaccgg tgccgatttt cacgcgatga ccgagagtca cgttgccctc
     7441 gatgataacg ttagtatcaa tttcaacatc gcgcccgtga gtaagcgtac cgcgcaaatc
     7501 aaaacgcgcc ggatcgcgca gcataacgcc tgctaacagc agtttttcag cctgttcgga
     7561 ctggtaaacg cgctccagac gggagagttg caggcggtta ttcacacctt ctacttcgct
     7621 taaacgttgc ggatgaacgg cgacgatttc acgcccttcc tgatacgcca gcgcaataat
     7681 gtcggtgatg tagtattcgc cctgagcatt attgttggtc agcttcgcca gccagcgttt
     7741 catatctgcg ccgttagcga tcagaatgcc ggtgttgatc tcctgaatct gacgctgctc
     7801 gtcggtggca tctttgtgct caacaatgcc ggtaactttg ccattttcac gggtgatacg
     7861 tccataaccg gtcggatcat ccagtttcac cgtcagcaga ccaatgccac cctgcggttt
     7921 agcatcacgc aggcgctgga gtgtttcgac agagatcagc ggcacgtcgc cgtagagcat
     7981 taaaatgtct tcatcatcgg caaagaaagg tgcggcctgc tgcattgcat gacccgtacc
     8041 cagctgctct gcctgaagca cccagttaag gttgtcgtct ttcagcgcct gtttaagcag
     8101 atcgccgccg tgaccgtaca ccaggtgaac gtgcgctgcg cctaattcat tcgcagcatc
     8161 aatgacatgc tgaaccatcg ctttcccggc aagggtatgc agcactttcg gaagatcgga
     8221 atacatgcgc gtgcctttgc ctgcggcaag gatcactacg ctcatagcat tattcaacat
     8281 acgcgtcctg actgtaattt gagaacgaat ttaaaccgct tcaccttgaa aaaactacat
     8341 ttttttcatc gtgaaatgga cagaggataa attgttcaat aacggattat ccccgctgac
     8401 gaaagtgcca ttttcgacca tcgtcacgcc ttttgtctgc agaaaattaa ggcagtacct
     8461 tcatttaagc gtcaggtgga tgtttttgct cttatttcga tcaatgaata aacgggaaaa
     8521 caggggtgtt ttatatcagc aggatctatg tgaacgctct tcaggacgga tcacgcgtaa
     8581 aaaaaagcca gcctgtttcc agactggctt ttgtgctttt caagccggtg ttacatcgct
     8641 tttttggtca actcgataac gcgcagctgc gcgatcgctt tggccagttc cgcagacgcc
     8701 tgagcgtaat ctacgtcgcc gtgagagctg ctaatgtgct cttcagcctt acgtttcgct
     8761 tccatggctc gcgcttcgtc gagatcctgg ccgcgaattg cggtgtcggc cagaacggtc
     8821 acgttgccag gctgcacttc aagaatgccg ccagacagat agataaactc ttcgtgaccg
     8881 tgctgtttca cgatgcgaat cataccaggc ttaatggcgg tgagcagcgg tgcgtggcca
     8941 gggtagatcc ccagttcacc ttcgctaccc gttacctgga ttttctcgac cagaccagag
     9001 aacatttgtt gctctgcgct gacgacgtcc aggtggtaag tcattgccat atcaccctcc
     9061 gattaaggcg ttaaagtttt ttggcttttt ccacagcttc ttcgatggaa ccgaccatgt
     9121 agaacgcctg ctccggcagg tgatcgtatt cgccttccat gatgccttta aagccacgga
     9181 tggtgtcttt cagggagacg tatttacccg gagaaccggt gaatacttct gccacgaaga
     9241 acggctggga caggaagcgc tggatcttac gagcacgcgc taccaccagt ttgtcttctt
     9301 cagacagttc atccataccc aggatggcga tgatgtcttt cagttcctga taacgttgca
     9361 ggatggactg aacgccacgc gcggtgtcgt agtgttcctg accaaccacc agcgggtcca
     9421 gctgacggct ggtggagtcc agcgggtcaa cggccgggta gatacccaga gacgcgatct
     9481 gacggctcag taccacggtt gcgtcaaggt gcgcaaaggt ggttgccgga gacgggtcag
     9541 tcaagtcatc cgcaggtacg tatactgcct gtacggaggt gatagaacca gttttggtgg
     9601 aggtgatacg ttcctgcaga acgcccatct cttccgccag ggtcggctga taacctaccg
     9661 ctgaaggcat acggcccagc agtgcggata cttccgtacc tgccagggtg taacgataga
     9721 tgttgtcaac gaacagcaga acgtcacgac cttcgtcacg gaatttctca gccatggtca
     9781 gaccggtcag agcaacgcgc agacggtttc ccggcggctc gttcatctgg ccatacacca
     9841 gggatacttt gtcgataacg ttggagtcgg tcatttcgtg gtagaagtcg ttaccctcac
     9901 gagtacgttc acctacgccc gcaaacacag agtaaccgga gtgctcgatc gcgatgttac
     9961 gaatgagctc catcatgttt acggttttac ctacacccgc accaccgaac agaccaactt
    10021 taccgccctt agcgaacgga cacatcaggt cgataacttt gataccggtt tccagcagtt
    10081 cctgagagtt tgacagctct tcgtaggaag gtgctgcgcg gtgaatcgcc caacgctctt
    10141 cttcaccgat ctcgcctttc atgtcgaccg gttcacccag tacgttcatg atacggccca
    10201 gagtcgcttt acctaccggg acttcgatcg ggtgttcgag gtcttttaca tccagaccgc
    10261 gacgcagacc gtcggaggaa cccattgcga tggtacgcac gataccgccg ccgagctgct
    10321 gctgaacttc cagcaccaga cgctcattac cattttgcac ctcaagagca tcgtacacgc
    10381 gcggtacggc atcctgaggg aattcgacgt caactacggc gccgattacc tggacaatct
    10441 ttccagtagc catcttaaat cctctacgaa ataacctgtt taaaccgcgg cggcccccga
    10501 gacgatctcg gtgagttcct gagtaatgct ggcctgacga gctttgttgt ataccaactg
    10561 cagctcttta atcaggctgc cgccattgtc ggtcgcggct ttcatcgcca ccatacgggc
    10621 ggcctgctcg ctggccaggt tttcaaccac gccctgataa acctgagatt cgacataacg
    10681 acgcagcagg gtatccagca acgccttcgg atcgggttcg tacaggtaat cccaggattt
    10741 atgtttcaga tcatcatcat ctgatgccgg taacggcagc agctggctga tggtcggaac
    10801 ctgagacatg gtgttaataa atttgttgct gacaatgtaa agcttgtcca gacggccttc
    10861 gtcgtaggcc tgcaacatca cttttaccgg accgatcagt tcggacaggg aagggttatc
    10921 ccccatgccg gtgacctggg caacaacatt gccgcccacg gagttgaaga acgacacgcc
    10981 tttcgagccg atcattgcga ggtcgcattg aacgcctttg tcggtccagg tcttcatttc
    11041 cgccagcagt tttttgaaca ggttaatgtt caaaccaccg cacaaaccac ggtcggtcga
    11101 caccaccagg tagcccacgc gtttaacgtc gcggtcttcc aggtaagggt gcttatattc
    11161 cagattaccg tgtgcaaggt gaccaatcac tttgcgcatg gtttctgcat aaggacggct
    11221 ggccgccatg cgatcctgcg atttacgcat tttggaagcg gcgaccatct ccatcgcttt
    11281 agtgatcttt tgcgtgttct ggacacttgc gatcttacta cgtatctctt ttgcgccggc
    11341 catgagcttc tcctcaatgc cttgcggcct gccctaaggc aagccgccag acgttaccag
    11401 gattgggttg ctttgaagga atcgaggatg cctttcagct tgccttcgat ttcgtcgttg
    11461 tagccaccgg tctggttgat ctcttgcatc aacggagcgt gatcacggtc gacgtaagcc
    11521 agcagagcgg cttcgaagct gccaattttc gacagttcaa catccgccag gtaaccacgt
    11581 tctgctgcga acagaaccag agactgctgc gcaacggaca tcggcgcata ctgtttctgt
    11641 ttcagcagtt cggtcacttt ctgaccgtgg tcaagctgct tacgtgttgc atcgtcaagg
    11701 tcggatgcaa actgagagaa cgctgccagt tcacgatact gtgccagagc ggtacggata
    11761 ccaccggaca gttttttcat gatcttggtc tgtgctgcac caccaacacg ggatacggaa
    11821 atacccgggt taaccgcagg acgaataccg gcgttgaaca ggttggtttc caggaagatc
    11881 tgaccatcgg taatggagat tacgttggtc ggaacgaacg cagaaacgtc acccgcctga
    11941 gtttcgataa tcggcagtgc ggtcagagaa ccggttttcc ctttcacttc acctttggtg
    12001 aaggcttcaa cgtattcggc gttaacacgt gcagcacgct ccagcagacg agagtggagg
    12061 tagaaaacgt cgcccgggaa tgcttcacgt cctggcggac gacggagcag cagggagatc
    12121 tgacggtaag caacagcctg tttagacagg tcatcgtaaa tgatcagcgc atcttcaccg
    12181 cggtcacgga agtattcgcc cattgcgcaa ccggcatacg gtgccaggta ttgcagtgca
    12241 gcggattcag acgcggttgc taccacaacg atggtgttag ccagtgcgcc gtgctcttcc
    12301 agtttacgta ccacgttaga aatggtggac gctttctggc cgatagcgac atagatacat
    12361 ttgataccgg aatcgcgctg gttgatgatg gcatcgatag ccagtgcggt tttacctgtc
    12421 tgacggtcac cgatgatcaa ttcacgctga ccacgaccga ttgggatcat ggagtcaacg
    12481 gctttataac cggtctgtac cggctgatct acggactgac gttcgataac gcccggagcg
    12541 attgcttcta cagcagagaa gccgtcgtga tccagcggac ctttaccgtc gattggtgca
    12601 cccagagtgt taaccacacg gcccagcagg ccacggccaa ccggaacttc caggatacgg
    12661 ccagtacact taactttcat gccttcggca aggtcagcgt acggacccat aacaaccgca
    12721 cctacagagt cgcgctcgag gttcagtgcg atagcgtaac ggttacccgg cagggagatc
    12781 atttcaccct gcatacaatc ggccaggccg tgaatgcgga taacaccgtc acttacagaa
    12841 acaatagtac cttcgttgtg agcttcactc acaacattga actgagcaat gcgctgcttg
    12901 atcagttcgc tgatttcggt ggaattcagt tgcatgctcc agtcccctta agactgcaag
    12961 acgtctgcaa ggcgctcaag acgaccgcgt acgctgccat caatgaccat atcacccgct
    13021 cggatgataa cgcctgccat tacagactta tcgattttgc aattcagctt aactttgcgt
    13081 gacagacgtt tttccatcgc agcagaaatt ttcgcgagct gttgttcact cagtgcggca
    13141 gcggaaatga cgtctacctc agcggtagcc tcactcacgg cacgaaggtg aataaactgc
    13201 tccagaacat ccgggagcgc gttaagacga ccattttcag ccattacccg aatcaggttc
    13261 tgaccgtttt cgtccagttg ctcaccacaa actgcgataa acgactcggc gagcgtttct
    13321 ggcgcaagcg cgccagagag aagctctgcc atttgttcgt ttttggttac ctcggcggca
    13381 aacgccagca tgtcctgcca gcgttctaca ctttggtgtt cgacggcaaa gtcaaaagct
    13441 gctttggcgt aggggcgagc taccgtaata aattcagaca tcagcccctc cctccttaca
    13501 gttcagcgac aagtttatcc acgatgtcgc tgttagcagc ttcatccacg gaacgttcga
    13561 tgatcttctc ggcgccagca acagccagga tagcaacttg cttacgcagc tcttcacggg
    13621 cacgtttacg ctcggcttca atttctgcct gcgcctgggc cacgatttta gtacgttcct
    13681 gttctgcctc agctttcgct tcgtccagaa tctgcgagcg gcgtttgttc gcctgctcga
    13741 tgattacctg ggcttccgct ttcgcttttt tcagctggtc ggtcgcgctg gcctttgcaa
    13801 ggtcaaggtc cttatgtgct cgttctgcgg aagcaaggcc gtcagcaatt tctttttgac
    13861 gtttttcgat ggctgccatt aatggcggcc atacgtactt catgcagaac agaacgaaca
    13921 ggacaaacgc gatggcctgg ccgaggattg ttgcgttaag attcacagca caatgcctct
    13981 atttagttaa cgttctgata ttgctcttta aataaaagca acgcttacta cgcgacagcg
    14041 aacatcacgt acagacccag acctacagcg atcatcggga tagcatccac cagacccata
    14101 acgataaaga actgagtacg cagcagagga atcagatcag gttgacgcgc tgcgccttcc
    14161 aggaatttac ccccgaggat gccgataccg atcgcagcac cgattgccgc cagacccatc
    14221 atcacagcgg cagccatgta cagcagatcc atattcaggt tttccatgac agtctccagt
    14281 ttgtttcagt taaaacgtag tagtgttggt aaattaatgt tcttcagacg ccatcgacag
    14341 atagacgatc gtcagaacca tgaagatgaa ggcttgcagc gtaatgatca ggatgtggaa
    14401 aatggcccac ggcacattca ggatccactg tgaccaccac ggcaacagac cagcaatcag
    14461 aatgaaaatc agctcaccgg catacatgtt accgaacagt cgcaaaccaa gtgaaactgg
    14521 tttggacagc aggcttaccc cttcaaggat taagttgaca ggaatgaacg cccagtgatt
    14581 gaacggctgc agcgtcaact ctttcgtgaa gccgccgatg cctttcattt tgatgctgta
    14641 gaacagaatc aggataaata cgcccaatgc catagacagc gttacgttca cgtccgcaga
    14701 cggaaccaca cgcagtgcag gcagacccag tacatgttca gcaatgtacg gcagcaggtc
    14761 gataggcagt aaatccatca ggttcatcag gaatacccag acgaagatcg tcagggccag
    14821 cggagcaatc agcttgcttt tgccatggta catgtctttc acgctaccat taacaaagcc
    14881 gatcaccagc tcaatcgcgg tctgaaactt acctggcaca ccgctggtcg cctttttggc
    14941 tacgctacgg aataaaacca ggaacaacag acccagcacc accgagaaga acatggagtc
    15001 aatattgatt gtccagaagg tggctggggg gttttgtgga tccaccagcg agaatgtacg
    15061 caggtccagc tgaaggttat tcaggtggtg tcctatgtaa tcctgcggcg tcatattttc
    15121 tgaagccatg atgcctttta ccctttgttg ttaattacag ccggtgccag tatctgaacc
    15181 accagcacca aaacccacgt aacgatcagc ggcaagaata ccgcctttaa aaccgccaac
    15241 gccaccacca gtaacaccaa catcgccaga actttgaaag cttcgccaaa tgcgaatgtc
    15301 caggccaccc ggcctttcgc tggtgtatgc gcctggtgac gccaggcaaa tatcataaac
    15361 aaaacgttag gcagaaagac tgccaggccc ccgcttattg cagagacgcc ccagaagggg
    15421 tctttgaggc tgaacagcaa tccacttgct atcaccacca gtaactgaac gagcagaagc
    15481 ttccgagcaa cgtttcgact cacgagcgac acagacatca cgtttttcac tcctgctccc
    15541 ttcgaggtat gccgcgtgtc gtataaaact ttctttaagg cttagagtca agcatcaaaa
    15601 agcggtcaaa ttatacggtg cgcccccgtg atttcaaaca ataagtagcc aaaaggtgaa
    15661 taaatgttta aatatttttc cagtgcatac aattgcgact tttctgctaa ccctgttcga
    15721 tcatgaaaaa ctgtaaataa cgcgtaaaca ctggtgataa agcgtgcttc agatcacata
    15781 ttgcgcatgt tcgcgcacag catatttatt tacttggcaa atgatgcctt tgcaagttta
    15841 tga
//
LOCUS       DAZFXT010000107        15811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.107, whole genome shotgun sequence.
ACCESSION   DAZFXT010000107 DAZFXT010000000
VERSION     DAZFXT010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.107"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..294)
                     /locus_tag="ACHKW0_003643"
                     /pseudo
     CDS             complement(1..294)
                     /locus_tag="ACHKW0_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(593..757)
                     /locus_tag="ACHKW0_003644"
                     /pseudo
     CDS             complement(593..757)
                     /locus_tag="ACHKW0_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(883..1284)
                     /gene="eptO"
                     /locus_tag="ACHKW0_003645"
                     /gene_synonym="gspS"
     CDS             complement(883..1284)
                     /gene="eptO"
                     /locus_tag="ACHKW0_003645"
                     /gene_synonym="gspS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001222321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspS family T2SS pilot lipoprotein variant EptO"
                     /protein_id="HFO7476409.1"
                     /translation="MRINTLKKLNCITSLLVLVTVAGCHQVPSTRKQATVPPSEQLEQ
                     TASIAAATRYLKRRCNRSDLPDDQAILNGVNRIANGKGWQSLTQEDIRKHSDEINERL
                     ARDSTPEHIKCSEFNRLLVPFIGELLAGTSR"
     gene            complement(1378..2136)
                     /locus_tag="ACHKW0_003646"
     CDS             complement(1378..2136)
                     /locus_tag="ACHKW0_003646"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HFO7476410.1"
                     /translation="MGSFLNVVIHRLPVILERRWRREALMLLAHSEPESEPVFNLMIP
                     RSHCPYCFQPLSIRDNIPLLSYLFLKGKSRCCGERIPIYYPLVEITSSVLFILAASRF
                     HPGLTLVAAWLFIAMLLVLAVIDCRTAMLPDVLTLSLLWLGLLFSLQGGRVPLEEAVV
                     GAVSGYLCLWGLYWLFRFVTGREGLGYGDFKLAAALGAWMGWQVLPSVLLFASVSGLI
                     ITVLLRFVTATDFTRPLPFGPWLALSGVCHFLLM"
     gene            complement(2263..2778)
                     /gene="gspM"
                     /locus_tag="ACHKW0_003647"
     CDS             complement(2263..2778)
                     /gene="gspM"
                     /locus_tag="ACHKW0_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspM"
                     /protein_id="HFO7476411.1"
                     /translation="MNDLKKRLQYISPRERRLLTGCVALLVVAFVYYVFWLPWQVRED
                     KWRTVIAREKYTVEWMKQQIPSIKKWEASMPEQGEPLGLSAAVTQTSAAYGVSVTRLQ
                     PQGERLTVTLAPVEFNTLMQWLTQLHRRYRIRTVVFDVVAQGNQPGHVTVNRLVLSRD
                     DINRDTSPGHL"
     gene            complement(2765..4042)
                     /gene="gspL"
                     /locus_tag="ACHKW0_003648"
     CDS             complement(2765..4042)
                     /gene="gspL"
                     /locus_tag="ACHKW0_003648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011321724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HFO7476412.1"
                     /translation="MERGAFPEHSATVWINGEGGTLNKNNKHVEAGRLFLRLPEDDTQ
                     PLLWRLVSPEETGGGSVAVGETDPLLAGLLPRYPAWVLVPSDRIAFHQVTLPHRTWRQ
                     RLQVLPFLLEEQLATDIEQLHFAILHQTGGRCNVAVVEKSVMHHWLTYCGQLGVREVR
                     LLPDVLMLPLATEGWSAVRLAGQWLFRSDRYAGMTVELSWLTQFLSLTAPPVIESYSV
                     PPAQCMGLESTEWRTQPVRDLLQLAAECEGYDGADLRQGIFARQGTWYARFRPWRYVA
                     GALLACVFLAGSNAGLAHYRLWQQAEFWQHESVRVYQELFPGETVVKDPHRQMLRHLQ
                     QSTSNNMPELGGVMHQLQQLLPETSTIRLQALAWDSSSQTLKIDLQAASFQALEHFQQ
                     SAGEKYIIQPGEIRQQPHGVESRLILRMNNERS"
     gene            complement(3976..4953)
                     /gene="gspK"
                     /locus_tag="ACHKW0_003649"
     CDS             complement(3976..4953)
                     /gene="gspK"
                     /locus_tag="ACHKW0_003649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HFO7476413.1"
                     /translation="MKSREQGVALLVVLLILSLMVTIAAAIAERNGRTFWRTVAQLDQ
                     LQAKWDGYAAEEMAIQILQRSRQASPRKTHLAQNWAQPELQFETGGGDVRGRIVDAQA
                     CFNLNAINYGAVDLAGVPYAARIFQQLLINLQVDIIQARQVTAALRDWIDRDDEPVKE
                     GAEDEVYMGMEPPYLAASQPMQDVSELRLIRGIDVRLYRKLLPYVCVLPTSDLSVNVN
                     TLLDSQAPLLAALFLTKPNSLLVTKLLLRRPRTGWDSVAAFLDSPQLKGIDTSTAISV
                     LAVSSNYFLVRLHVRSGEHLFSQQTLMQWREERFRSIQRQYGLTVREVP"
     gene            complement(4950..5549)
                     /gene="gspJ"
                     /locus_tag="ACHKW0_003650"
     CDS             complement(4950..5549)
                     /gene="gspJ"
                     /locus_tag="ACHKW0_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HFO7476414.1"
                     /translation="MSQQRVKGFTLLEMLLALAVFAALSISAFQVLQSGIRAHELSQD
                     KVRRLAELQRGGSQIERDLMQMIPRHSRGSEGLLLAAPHLLKSDDWGISFTRNSWLNP
                     AGMLPRPELQWVGYRLRQQKLERLSYFYVDHPSGIAPDVRVVLEGVHAFRLRFFVNGT
                     WQARWDSTSILPQAVEVTLVMDDFAELTRLFLVSKETAE"
     gene            complement(5546..5911)
                     /gene="gspI"
                     /locus_tag="ACHKW0_003651"
     CDS             complement(5546..5911)
                     /gene="gspI"
                     /locus_tag="ACHKW0_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HFO7476415.1"
                     /translation="MKRQEGMTLLEVMVALVVFALAGIALIQSTTQQAAGIGPMEEKV
                     LAGWLADNQMVQLQLEKIWPKNGWDEKTISFAGREWYLRWHGRDSDIPEQRILEVEVR
                     RTREETSALVSLRGSVVRE"
     gene            complement(5908..6459)
                     /gene="gspH"
                     /locus_tag="ACHKW0_003652"
     CDS             complement(5908..6459)
                     /gene="gspH"
                     /locus_tag="ACHKW0_003652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HFO7476416.1"
                     /translation="MSQRGFTILEMMLILLLMGITAGLVLMSFPDSAQNHLQQQRERL
                     QAQLDYALDRSMQDGLLIGIQVRSDGWTFKVLQRVTAESFPASVGGDVWQGYVWQTWQ
                     PRRAAMEGKLPDDVRLDLQYLRGLQWMPEHNDGTGPDILLLPGGEVTPFSLLFRLVGG
                     EAVTGLQVDENGLMTLFEGEVEL"
     gene            complement(6456..6890)
                     /gene="gspG"
                     /locus_tag="ACHKW0_003653"
     CDS             complement(6456..6890)
                     /gene="gspG"
                     /locus_tag="ACHKW0_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HFO7476417.1"
                     /translation="MRKQHQRGFTLLEIMVVIVILGVLASLVVPNLMGNKDKADRQKV
                     VSDLVALESALDMYRLDNNRYPTTEQGLRALVSKPTVQPEPRNYRQDGYIRRLPQDPW
                     GGDYQLLNPGQYSNIDIFSPGPDGIPNTEDDIGNWTPGHTQP"
     gene            complement(6921..8144)
                     /gene="gspF"
                     /locus_tag="ACHKW0_003654"
     CDS             complement(6921..8144)
                     /gene="gspF"
                     /locus_tag="ACHKW0_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system inner membrane protein
                     GspF"
                     /protein_id="HFO7476418.1"
                     /translation="MALFHYQASDIHGRKRRGVLEADSARHARQLLREQALIPVRLEE
                     KQLHHQHSPRSILRFRPRGVSSAELALLTRQLSTLVAASLPLEEALDALSRQSEKPRQ
                     RDLIAAVRARVLEGHSLATAMGMFPGTFERLYCAMVAAGETSGRLDVVLSRLADYTEQ
                     RQIMRNRLLQALIYPCVLTLVAVGVIAILLTAVVPKVVEQFIHMKQTLPLSTRVLMGL
                     TEVCQTWGPWLLLAAATGWIAGRMMLHKPSRRLAFHHLLLRLPIVGRISRGLNTARYA
                     RTLSILNASAVPLLQAMYISGDVLSNDWARHQLATAAELVREGVSLHQALEQTSLFPS
                     MMRHMIASGENSGELDSMLERAADNQDREFSAQMQLALGLFEPMLVVGMAGVVLFIVL
                     AILQPLLQLNNMMNI"
     gene            complement(8146..9648)
                     /gene="gspE"
                     /locus_tag="ACHKW0_003655"
     CDS             complement(8146..9648)
                     /gene="gspE"
                     /locus_tag="ACHKW0_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system ATPase GspE"
                     /protein_id="HFO7476419.1"
                     /translation="MSSIQNVSESRPLLPFSFSRTQRILLLREQEGNQVFCMEDTPAS
                     ALLEVRRVAEGPLNVTTLSAEAFEKQLVSCYQRDSDEARQMMADIGNEMDFYTVAGEL
                     PDSEDLLDANDDAPIIRLINAMLTEAIKEKASDIHIETYERHLQVRFRVDGVLREILR
                     LRRNLASLLISRIKVMARLDIAEKRVPQDGRMVLRIGGRAVDVRVSTLPSNHGERIVL
                     RLLDKNSVSLDLAALGMSPQNQRHIDALIRRPHGIILVTGPTGSGKSTTLYAALSRLN
                     PQDRNIMTVEDPVEYELDGISQTQVNPKVDMTFARSLRAILRQDPDVVLVGEIRDSET
                     AQIAVQASLTGHLVLSTLHTNSAAGALSRLQDMGIPPFLLSTSLLAVLAQRLVRTLCP
                     RCRQSCQASAELAKDMDIPPETTIWQPAGCQHCSFTGYHGRTGLHELLLIDDRIRMAI
                     YQGEGELSITRLAGGRYLTLRGDGRQKVLAGETSWEEVVRVTESRLQEEE"
     gene            complement(9645..11615)
                     /gene="etpD"
                     /locus_tag="ACHKW0_003656"
     CDS             complement(9645..11615)
                     /gene="etpD"
                     /locus_tag="ACHKW0_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="variant type II secretion system secretin EtpD"
                     /protein_id="HFO7476420.1"
                     /translation="MFRKWLNGRLSIFVFTTVFLSAIPGWGAEFSASFKDTDIQEFIN
                     TVSKNLHKTVIINPDVQGTITVRSYDMLNEEQYYQFFLSVLDVYGFAVVDMHNGILKV
                     VRSKDAKTSAVPVASDASPGTGDEVVTRVVPVSNVAARDLAPLLRQLNDNAGAGSVVH
                     YEPSNVLLMTGRAAVVKRLMEIVERVDKEGNRSVATVPLTYASATDVARLVTELTKES
                     DKTAMPAWMTAKLVADERTNSVLVSGEPISQQRIISMIKQLDRKEDVQGNTKVIYLKY
                     AKAKDLVEVLTGISSNIENDAKKSSSTEALRKGVTIKSHEQTNALILTGAPDVIRDLE
                     NVISQLDIRRPQVLVEAIIAEVQDADGLNLGIQWVNKHSGVTQFTSTGLPITTMVQTR
                     QSDISDSDQSNTLSMFNGIAAGFYQGNWAMLLTALSTSSKNDILATPSIVTLDNMEAT
                     FNVGQEVPVLSGSQTTSGDNIFNTVERKTVGIKLRVKPQINEGDSVLLEIEQEVSGVA
                     DTAVATTTDLGATFNTRTVTNAMLVGNGETVVVGGLLDKSIRGSESKVPLLGDIPVLG
                     HLFSSKSEHAAKRNLMLFIRPTIIRERDGFRHASAEKYLSFNQEQVQSRGKETMALTL
                     NEEQLKLSRDRDDTTFRKVKTAIAAFYARGMQ"
     gene            complement(11616..12491)
                     /gene="gspC"
                     /locus_tag="ACHKW0_003657"
     CDS             complement(11616..12491)
                     /gene="gspC"
                     /locus_tag="ACHKW0_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspC"
                     /protein_id="HFO7476421.1"
                     /translation="MLFFLSSRRDRNLFIKDIALKMLTPNWVLCVILLIAGYQLVSVI
                     RHFWLTPATSASDLSHVSVSETAVTDEHTEENFVFTLFGTASPPLSEGKVQKTTSSLS
                     DDLLSGGDLDVRGILYSSVTEHSVAIFAHNNRQFSLGIGEKVPGYDATISAIFSDHIV
                     INYQGKNASLPLRYDNPAKRNAQDDNNLIVGPVTTQANFRVKNIFDIMSLSPVTVNNT
                     LSGYRLSPGKASSLFYNAGLHDNDLAVLLNGSELRDTRQAKQIMKQLTELKEIKITVE
                     RDGQLYDAFIAVGEN"
     gene            complement(12575..15235)
                     /gene="stcE"
                     /locus_tag="ACHKW0_003658"
     CDS             complement(12575..15235)
                     /gene="stcE"
                     /locus_tag="ACHKW0_003658"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M66 family glycomucinase StcE"
                     /protein_id="HFO7476422.1"
                     /translation="MKLKYLSCMILASLAMGAFAATAADNNSAIYFNTTQPVNDLQGG
                     LAAEVKFAQSQILSAHPKEGESQQHLTSLRKSLLLVRLVKADDKTPVQVEARDANDKI
                     LGTLTLSPPSSLPDTVYHLDGVPADGIDFTPQNGTKKIINTVAEVNKLSDASGSSIKS
                     YLANNALVEIQTANGRWIRDMYLPQGAELEGKMVRFVSYAGYNSTVFYGDRKVTLSVG
                     NTLLFKYVNGQWFRSGELENNRIAYAQHTWSAELPAHWIVPGLNLVIKQGNLSGSLND
                     INVGAPGELLLHTIDIGMLTTPRGRFDFAKDKEAHREYFQTIPVSRMIVNNYAPLHLK
                     EVMLPTGTLLTDADPGEGGWHSGTMRQSIGKELISHGIDNANYGINSTAGSGESSHPY
                     VAAQLTAHTNRGNYSNGVQVHGGSGGGGIVTLDSTLGNEFSHEVGHNFGLGHYVGGFR
                     GSVHRSADQINSTWGWDSDKKRFIPNFYPAQTNRKSCLDEQCQEPFDGHSFGFDAMAG
                     GSPFSDANRFTMYTPNSSAIIQRFFENKAVFDSLSSTGFSKWNADEHKMEPYEHTIDR
                     FEQITASVRDLSENKMAEMMAEYPIVKVHMSDGNWTRDIHAPAASAENKGRILTLDHE
                     AGYNSHLFINGGEKIISKGYKKSFVSDGQTWKESDVVNTREARKPEKFGVPVTTLVGY
                     YDPKGTLSSFIYPAMYGAYGFTYSDDSENLSSNDCQLQVDTREGQLRFKLANNRVNSS
                     VMNKFHINVPTESQPTQAILVCNNKVLDTRTLTPAPEGLTYTVNGRALPAKENEGCIV
                     SVKTAQRYCLPVGQRSGSSLPDWIAGQEVYVDSGAKAKVLLSDWDNLSYNRIGEFVGN
                     VNPADMKKVKAWNGQYLDFSRPRSMRVVNK"
BASE COUNT         3988 a         4476 c         3495 g         3852 t
ORIGIN      
        1 ctattctccg gtcatctgca gcagtttttt ggcatcgtga cttctgagca acggaatgcc
       61 agcctcataa cctcttccag actaatatcc cggcgccagc gttccagtcg aaaatgacag
      121 gtatcatcca gctctactgc tgcaacaata ctgaacgagc gccgttcaac ggacgatatc
      181 agcccgtacc tgcggacaca gataccacca tttcatatcc acgattttgg acagtccttt
      241 cataaaactg acttcccttt ctgtcagcag cgtcttctga cctcaccagt attcactctg
      301 catcaccgat gccgtataca tgaacaagac aaattggcgt gatggttatg tgaaagagga
      361 gaaaatgccg atgtttcagt taggtattga tgtcagtaaa aacaatcttg atctatgcct
      421 cttacggggg acattaaagg gaagatcaaa actcgtaagc taaaaaatac cgctgatgcg
      481 gctaaaactg tggcagagtg gcttaaaaag caatccagtg aactgaacga agttcatatc
      541 atcatggaag ccactggggt atatcatgaa cctctagctt atggccttca ctttgctggc
      601 gctcagtggc agttagtcca tcattaccgc tcccggtagc ccgctcatgc tggcgccccc
      661 agacacgtag agtttctggc gtacagctaa tctttggggc aatgaaacaa attgccgccc
      721 gttgtaagtc atattcgccc tgacgcacca gaatcatacg gaccgtccgc taacggactt
      781 ccggggaaaa acgtgtattt ttggtcatcc tgtttacctc tttctcaggg agtttagtct
      841 cctagatttc cgaggcgcgg ttcagtacct gtcagtaccc tattatctgc tagtccctgc
      901 cagtaattca ccgataaatg ggaccaacag ccgattgaac tcactgcatt ttatgtgctc
      961 aggtgtgctg tccctagcca gacgttcatt gatctcatcg ctgtgtttac gtatatcctc
     1021 ctgagttaag gactgccacc ctttcccgtt agcgatccgg ttaacgccat tcagtatcgc
     1081 ctgatcatct ggcaaatcac tgcggttaca tctccttttc agataccgtg tcgcagcggc
     1141 gatcgacgcc gtttgttcta gctgttcgct cgggggaacg gtggcctgct tacgagtgga
     1201 aggtacctga tggcaaccgg cgacagtcac caggaccagt aatgacgtga tacaatttag
     1261 ttttttcaac gtattaatcc tcatattttt aaccaggtat tgtttaaccg gtttccggtt
     1321 tactatagtc ccgttattct ctgagccatt tttattacca gccccggaaa ccgaacgcta
     1381 catcaacaaa aaatggcata ctccgctcaa ggcgagccac ggaccaaaag gcagcggccg
     1441 ggtaaaatcc gtggctgtaa caaaacgcag cagtaccgtt atgattaatc cgctgactga
     1501 ggcaaacaga agcacggagg gtaatacctg ccaccccatc catgctccca gtgccgcagc
     1561 tagcttaaaa tcaccgtagc ctaacccttc tctaccggta acgaaacgaa aaagccagta
     1621 cagcccccac agacagagat acccagagac cgcccccact accgcctcct ccaagggcac
     1681 tctccccccc tgtaagctga ataacaggcc aagccagagc agggataacg tcagcacatc
     1741 cggcagcata gccgtacgac agtcaataac cgccagaacc aacagcatgg caataaaaag
     1801 ccaggcagca actaatgtca gcccgggatg aaaccggctc gctgccagaa taaataacac
     1861 tgaactcgtt atctcgacca gcggatagta aataggaata cgctcaccac aacagcgtga
     1921 tttccccttc agaaagagat agcttaacag gggaatgtta tccctgatgc tgagcggctg
     1981 aaaacagtag ggacaatgag agcgcggtat catcaggtta aagaccggct cggactcagg
     2041 ctcagaatgt gccaacaaca tcagcgcttc acgccgccac cgcctctcca gtattaccgg
     2101 cagacgatga atcacaacgt taagaaaact accgataatg ccccctagcc cccctgtgct
     2161 gatgagccac aacaaatgaa aatcagccgc aaattccggc aacctgctca catatcctcc
     2221 tgcttatcac tcccattttc acgtcaggca ttaacctgta aatcacaagt gccccggcga
     2281 agtatccctg tttatgtcat cccgggacaa aaccagccga ttaaccgtta catgtccggg
     2341 ctggtttccc tgcgcgacca catcaaatac cacggtacga atacggtacc tgcgatgcag
     2401 ttgcgtaagc cactgcatca gcgtattaaa ttcaactggt gctaaggtaa cggtcagacg
     2461 ctccccctga ggctgcaatc gggtgactga tacaccatat gcggctgaag tttgagtcac
     2521 tgccgcagat aaacctaacg gctccccctg ttctggcata ctggcctccc atttctttat
     2581 gctgggtatc tgttgtttca tccactccac tgtgtatttt tcacgggcaa ttacagtccg
     2641 ccacttatcc tcccgtacct gccagggcag ccagaagaca taatagacaa aagcgacaac
     2701 aagtaaggca acacatccgg taagcagcct tcgctcacgg ggactaatat actgcaaacg
     2761 ttttttaaga tcgttcattg ttcattctca ggatcagacg actttccacc ccgtgcggct
     2821 gctgccgtat ctcgccaggc tggatgatat atttttctcc agcgctctgc tgaaagtgtt
     2881 ccagagcctg aaaagaggct gcctgcagat ctattttcag agtctggctg ctactgtccc
     2941 aggcgagtgc ctgtaagcgt atcgttgagg tttctggcag cagttgctga agctggtgca
     3001 tcacgccccc tagttccggc atattgttac tcgtcgactg ttgcagatgt ctcagcatct
     3061 gccggtgggg atccttcacc accgtttcgc caggaaacag ctcctgatag acgcgtacgc
     3121 tctcatgctg ccaaaattca gcctgctgcc agagccggta atgtgccagc cccgcgttgc
     3181 ttccggccag aaacacacag gccaacaggg cccctgcgac ataccgccat gggcggaatc
     3241 gcgcatacca tgttccctgg cgagcaaata ttccctgtcg cagatctgcg ccatcatatc
     3301 cctcacattc agccgccaac tgcagcagat cccgtactgg ctgagttcgc cactccgtgc
     3361 tctccaagcc catgcactgt gctggcggca cggagtaaga ctcaataacc ggcggcgcag
     3421 taagagacaa gaactgtgtc agccacgaca actccaccgt catcccggcg taacgatcac
     3481 tacgaaatag ccactgaccg gcaagcctta ctgcactcca tccttcagtt gccaacggca
     3541 gcatcagtac gtcaggcagc aacctcacct cccggacccc aagttgtcca cagtacgtca
     3601 gccagtggtg catgaccgat ttttcgacca cagccacatt acacctgcca cctgtctgat
     3661 gaagaatggc gaaatgcagc tgctcaatat ctgttgccaa ctgctcttcc agaagaaagg
     3721 ggagcacctg caaacgctga cgccacgtac ggtgtggcag cgtgacctga tggaaggcaa
     3781 ttctgtctga cggaaccagc acccaagcgg gataacgagg cagcagacct gctaacagcg
     3841 gatctgtttc tccgactgcc acgctgccgc cccctgtctc ctccggcgaa accaacctcc
     3901 acaataaagg ctgcgtgtca tcctccggca gtcgcaaaaa aagccggcct gcctcgacat
     3961 gtttgttatt cttgttcagg gtacctccct caccgttaat ccatactgtc gctgaatgct
     4021 ccggaaacgc tcctctctcc actgcattaa agtctgctgg ctgaacaggt gctccccaga
     4081 gcgcacatgt aaccggacca aaaaatagtt gctgctaaca gccagtaccg atatggctgt
     4141 actagtgtcg atgcccttaa gctgtggcga gtcaagaaac gctgccacgc tatcccaacc
     4201 ggtacggggt cgccgcagca gtaatttcgt caccaaaaga ctgttcggtt tcgtcagaaa
     4261 gagtgcagcc agcaacggcg cctgtgaatc cagcagagta ttcacattca ctgacaggtc
     4321 tgacgtgggc agtacacaaa catacggtaa aagtttgcgg taaagtctga catctattcc
     4381 ccggatgagg cgcaactcac tgacgtcctg catcggttgg ctggccgcaa gatagggggg
     4441 ctccatcccc atatacacct cgtcttcagc cccctcctta actggttcgt catcacgatc
     4501 gatccagtca cgcagagctg ctgtcacttg cctggcttgt ataatatcga cttgcaggtt
     4561 gattaacagc tgctgaaaaa ttctggcggc ataaggcaca ccggctagat ccactgctcc
     4621 ataattaatg gcattaagat taaaacaggc ctgtgcatcc actatccgtc cccgaacatc
     4681 tcctccgccg gtttcaaact gtagttctgg ctgcgcccag ttttgtgcca gatgcgtttt
     4741 ccggggcgat gcctgtctgc tcctctgtaa tatttgtatt gccatctcct ctgcagcata
     4801 gccatcccac tttgcctgca gctgatccag ctgcgccacc gtacgccaaa atgtcctgcc
     4861 gttacgctcc gcgatcgcag ccgcgatcgt caccattaac gacaagatca gcaatacaac
     4921 cagaagcgcc acgccctgtt cccgcgactt cattccgcgg tctccttact tacaagaaac
     4981 agacgggtaa gttcagcaaa atcatccata accagcgtca cctcaacagc ctgcggcagg
     5041 atgctggtac tgtcccagcg cgcctgccaa gtgccgttga caaaaaaacg cagacgaaat
     5101 gcgtgaaccc cctccagcac caccctgaca tccggagcaa taccagaggg gtgatcgaca
     5161 taaaagtagc tcaggcgttc taacttttgc tgtcgcagcc ggtaacctac ccactgtaac
     5221 tccgggcgtg gtagcatccc ggcgggatta agccaactgt ttcgcgtaaa ggaaatcccc
     5281 cagtcatcgc ttttcagcag atgaggagca gcgagcagca ggccttcact cccccggcta
     5341 tgtcttggga tcatctgcat aagatctcgc tctatctggc tgccccccct ctgcagctca
     5401 gccaaacgcc gtactttatc ttgggacagc tcgtgcgcgc gtattccgct ctgtagtacc
     5461 tgaaatgcgc tgatgctcag cgctgcaaaa acagccagtg ccaacagcat ttccagtagc
     5521 gtaaaacctt tcacccgctg ctgactcatt cacgaaccac actaccacgc aaggaaacta
     5581 aggcggatgt ctcctctctg gtacgccgta cctctacctc taggatgcgt tgctctggta
     5641 tgtcgctgtc cctcccgtgc cagcgtaaat accattctct gccagcgaag ctgatcgtct
     5701 tctcgtccca gccgtttttc ggccagattt tctcgagctg cagttgcacc atctggttgt
     5761 cagccaacca gccagccaac accttctctt ccatcggccc gatccctgcg gcctgctgag
     5821 tcgtggactg aattagagca attcctgcca gcgcaaacac aaccaaggcc accatgactt
     5881 ctagtaatgt cattccttct tgcctcttca caactcgacc tcaccttcaa atagggtcat
     5941 caggccgttc tcatcaacct gcagcccagt aacggcctcc cctcctacca gcctaaagag
     6001 aaggctgaac ggcgtcacct cacctccagg cagcaacaaa atatcgggcc ctgtcccgtc
     6061 attatgctca ggcatccact gcagacctct gagatactgc agatcaagac gcacatcatc
     6121 cggaagtttg ccctccatgg cagcacgccg cggttgccaa gtttgccaga catacccttg
     6181 ccaaacatcc ccccccactg atgccgggaa gctttcagct gttacgcgct gcaaaacctt
     6241 aaatgtccac ccgtcagacc ggacctggat cccaatcagc aagccgtcct gcatgcttct
     6301 atccaaggca taatccagct gcgcctgcag acgctcccgc tgttgttgca aatgattctg
     6361 cgctgagtca ggaaatgaca ttagtaccaa ccctgcagta atgcccatca acaaaaggat
     6421 cagcatcatc tccagtattg tgaatccgcg ctgactcacg gctgcgtgtg accgggcgtc
     6481 cagttaccaa tatcatcttc cgtgttcggt ataccatccg ggccgggaga gaagatgtca
     6541 atattgctgt actgaccagg atttaacagc tgataatccc ccccccaggg atcttgaggc
     6601 aaacgacgta tataaccatc ttgccgatag ttacggggct caggctgcac tgtaggttta
     6661 ctgaccagtg cccgtagccc ctgctccgtt gttgggtaac ggttgttatc taaccgatac
     6721 atatcgagcg cgctttccag cgcaaccaga tcgctaacca ctttctgccg atcggcctta
     6781 tccttatttc ccatcagatt cgggaccacc agactggcca gaactcccaa gatcacaatt
     6841 accaccatta tttccaacaa ggtaaaacca cgctgatgct gttttcgcat gtttttctcc
     6901 gttgactatt aaagaagaat tcaaatattc atcatattgt tgagttgcag gagcggttgc
     6961 agaattgcca gaacaataaa caagacgacg ccagccatcc ccaccaccaa cattggttca
     7021 aacaatccca gcgccagctg catctgtgcg ctgaattcac gatcctgatt atcggcggcc
     7081 cgttccagca tgctgtcgag ttcgccacta ttttcaccgc tggcaatcat gtgccgcatc
     7141 atggacggaa atagcgaggt ctgctccagc gcctgatgca ggctgacccc ttcccgaacc
     7201 aattcggctg cggtggctaa ctgatgacgg gcccagtcat tactcaacac gtctccactg
     7261 atgtacatag cctggagtag cggcaccgca ctggcattaa ggatgctcag cgtgcgggca
     7321 taacgtgcgg tattcagccc acgcgatata cgccccacga tcggcaggcg cagcagcaga
     7381 tgatgaaaag ccagacgccg ggagggcttg tgtaacatca tacgccccgc tatccagccc
     7441 gtggctgcgg ctagcagcag ccacgggccc caagtctggc agacctcagt cagccccatc
     7501 agtacgcggg tagacagagg aagggtctgt ttcatatgaa taaactgctc aaccactttc
     7561 ggcacaacag cagtaagcag aatggcaatc acccctaccg ctaccaacgt caggacacag
     7621 ggatagatca gtgcctggag cagacggttt cgcatgatct ggcgttgctc ggtatagtca
     7681 gccagacgac taagtaccac atccaagcga ccggatgttt cacccgccgc caccatcgca
     7741 caataaagac gctcaaacgt accaggaaac attcccatcg ccgtggccaa ggaatgtccc
     7801 tcaaggaccc tagcgcgcac ggcagcgatt aaatcccgct ggcgaggttt ctcactctgc
     7861 ctcgacagcg cgtccagcgc ctcttcaagc ggcagtgatg cggccaccaa ggtggataac
     7921 tgccgtgtca ataatgcaag ctcggcgctg ctcaccccac gaggacgaaa tcgaaggata
     7981 ctccgcggtg agtgctggtg atggagctgt ttctcttcca gcctaactgg aataagcgcc
     8041 tgctcacgca gcaactggcg ggcatgtcga gctgaatccg cctccagaac gccacggcgt
     8101 tttctaccgt gaatatccga tgcctgataa tgaaacaacg ccatatcatt cctcttcctg
     8161 caacctgctt tcagtaacgc gaaccacctc ctcccaactg gtctcaccgg ctagcacctt
     8221 ttgtcgcccg tcgccacgca gcgtcagata gcgcccccca gccaagcggg taatactcag
     8281 ctctccttcc ccctgatata ttgccatccg gatgcgatcg tcaatcagca acagttcgtg
     8341 gagcccggta cgcccgtgat agccagtaaa actgcagtgc tgacacccgg caggctgcca
     8401 aatcgtggtt tccgggggaa tatccatgtc cttcgccagc tcagcactgg cctgacagga
     8461 ctgacgacag cgcggacaca gcgtgcgaac tagtcgttgg gctagaacag ccaacaatga
     8521 ggtggaaagc agaaaggggg ggatgcccat atcctgcagg cgcgataacg cgcctgcagc
     8581 gctgttcgta tgtaacgttg acagcaccag atgcccagtc agtgaggcct gtacagcaat
     8641 ctgggctgtt tcactgtcac ggatctcccc tacaagcacc acatctggat cctgacgtaa
     8701 aatagcacgc aggctcctgg caaaagtcat gtccaccttc gggttgacct gggtctgact
     8761 gataccgtcc agttcatatt caacaggatc ctctactgtc atgatgttgc ggtcctgtgg
     8821 attcagccgg cttaacgcag cataaagcgt cgtgcttttc cccgacccag ttggcccagt
     8881 caccaaaata atgccgtgag gacggcggat cagcgcatca atgtgtctct gattctgcgg
     8941 cgacatgccg agtgcagcca gatcgagact aacgctgttt ttgtctagta aacgcagcac
     9001 gatgcgttca ccatggttcg aaggcaacgt tgaaacacgc acatccacag cccgtccgcc
     9061 tatacgcagc accatgcgac catcttgggg aacgcgtttt tcggcaatat ccagccgagc
     9121 cataacttta atgcgtgaaa tcagtaacga agccagattc cggcgtaaac ggaggatctc
     9181 ccggagaacg ccatcaacgc gaaagcgaac ctgtagatgg cgttcgtacg tttcaatatg
     9241 aatatctgag gctttctctt taattgcctc agtcagcatg gcgttaatca ggcggataat
     9301 aggcgcatcg tcattcgcat ccagtaagtc ctcactgtca ggcaactccc ccgcgacggt
     9361 ataaaaatcc atttcattac caatatcagc catcatttgt cgcgcttcat cagaatcacg
     9421 ctgatagcag ctcaccagct gtttttcaaa tgcctcagcc gacagagttg tgacattcag
     9481 aggaccttca gctaccctgc gaacctctag cagggcactg gcaggtgtat cctccatgca
     9541 aaacacttgg ttcccttcct gttcacgcag caacaaaatt cgctgtgtac gggaaaagga
     9601 aaaagggaga agggggcgtg attcactgac gttctgtata ctgctcattg catcccccgc
     9661 gcataaaacg cagcaatcgc cgttttcact ttccgaaaag tcgtatcgtc ccgatctcgg
     9721 gatagcttga gctgttcctc attcagcgtc agcgccattg tttctttacc acgcgactgc
     9781 acctgctcct gattaaacga caggtatttt tcggccgaag catgacgaaa tccgtcgcgt
     9841 tcacgaataa ttgttggccg aatgaacaac atcagattac gcttggctgc atgttcactt
     9901 tttgagctaa aaagatgtcc cagtactggg atatccccca gcagaggtac tttactctca
     9961 ctccctctga tcgacttatc cagtaatccg ccgaccacca ccgtttcgcc attccctacc
    10021 agcatagcat tggtcactgt ccgggtattg aaggttgccc ccaagtcagt agtggtagct
    10081 actgctgtat ctgccacacc ggacacctcc tgctctatct ccagtaacac tgaatcaccc
    10141 tcgttgatct ggggttttac cctgagtttg atccccaccg ttttacgctc gacggtgtta
    10201 aaaatattgt ccccagaggt ggtctgtgaa cccgaaagca ccggaacttc ctgaccaaca
    10261 ttgaacgtgg cctccatatt gtccagcgta acaatactgg gggttgccaa gatatcattt
    10321 ttgctacttg tggagagcgc cgtcagcagc atcgcccagt ttccctgata aaaccctgct
    10381 gcaatcccgt taaacatgct caaggtattg ctctgatcgc tgtcagaaat atcgctctga
    10441 cgggtctgaa ccatcgtggt aatgggtaaa ccagtactgg tgaactgcgt cacaccggag
    10501 tgtttattca cccactgaat ccccagattc aacccgtcag catcctgtac ttcagcaatg
    10561 atggcttcca ccaggacctg aggacgacga atatccaact gcgaaattac attttccaag
    10621 tcgcggatga cgtcaggagc ccctgtcagg atcaaggcat tggtttgttc gtgggattta
    10681 atcgtcaccc cttttcgcag agcttccgtt gacgagctct ttttagcgtc gttttcaata
    10741 ttgctgctga tgcccgtcag gacttccact aaatccttcg ccttcgcata tttcaggtaa
    10801 atcaccttcg tattaccctg aacatcctcc ttacggtcca gttgcttaat catggagatg
    10861 atacgctgtt gggagattgg ctctccgctg acgagaactg agtttgtccg ctcgtcagca
    10921 accagcttcg ctgtcatcca ggctggcatg gctgtcttat cactttcttt agtcagttcc
    10981 gtgacaagtc tggctacatc cgttgcagag gcgtaggtaa gtgggaccgt ggcaacgctg
    11041 cgattgccct ccttatccac ccgttcaaca atctccatca gccgtttcac cacggcagca
    11101 cgaccagtca tcagcagaac gttagagggt tcataatgca ctacgcttcc tgcgccagca
    11161 ttatcattga gctgacgcag caagggcgcc aagtctctgg ctgccacgtt actgacgggg
    11221 accacccggg taacaacctc atcaccagtc ccagggctgg catcactagc taccggcacc
    11281 gccgacgttt tagcatcttt tgaacgcacc actttcagta taccgttgtg catatcgacc
    11341 acggcaaaac cataaacatc cagcacgctg agaaagaact gataatattg ttcctcgttc
    11401 agcatatcgt agctgcgtac agtgatggtc ccctgcacat caggattaat tattaccgtt
    11461 ttgtgcagat ttttgctgac agtattaatg aactcctgaa tatccgtatc tttaaagctg
    11521 gccgaaaatt cagcccccca tcccggaatg gcactcagaa atactgtcgt gaatacaaat
    11581 atcgacaagc gcccgttcaa ccatttcctg aacaatcagt tctctcctac agcaataaag
    11641 gcatcataca gttgaccatc acgttcgacg gttattttta tttctttaag ttctgtcaat
    11701 tgtttcatta tctgcttcgc ctgtctggta tcacgcagtt ctgaaccatt aagcaatacg
    11761 gccagatcgt tatcatgcaa cccagcatta taaaataacg aactggcctt accggggctc
    11821 aagcgataac cgcttaatgt attattcacc gtgacaggtg aaaggctcat tatgtcaaaa
    11881 atatttttta cccgaaaatt agcttgggtt gtcacgggtc cgactattaa attattatcg
    11941 tcctgtgcat tcctttttgc tggattatca tatcgcagcg gcagtgatgc attttttccc
    12001 tgatagttaa taacaatatg atcgctaaaa atagcactaa tcgtagcatc gtaaccaggt
    12061 actttttcgc cgatgcctag actgaactgt ctgttattat gtgcaaatat ggcaacggaa
    12121 tgctctgtaa ccgaactgta aagtatacct ctcacatcaa gatccccccc tgaaagcagg
    12181 tcatcagaca gggaagatgt tgttttctgc actttccctt ccgataacgg cggggatgcc
    12241 gtgccgaata acgtaaacac aaaattttct tctgtgtgct catcggttac cgctgtttcc
    12301 gacacgctaa catgggataa atcggacgcc gatgtcgccg gagtcagcca gaaatggcgt
    12361 ataaccgaga ccagctgata tccggcaatg agtaaaatca cgcacaacac ccagtttggc
    12421 gtaagcattt taagtgcgat atcttttatg aataagttcc ggtctctccg ggatgataga
    12481 aagaacaaca taaaacaccg ggcgctattc taggttttga tgaacggaac gaatgtatta
    12541 ccgatgcgac ctggtggccg caccgtcttc cggattattt atttacaacc ctcatcgacc
    12601 tcggtctgct gaagtccagg tactgcccgt tccaagcttt aacttttttc atatcagctg
    12661 gattcacatt acccacaaac tcaccaatcc tgttatagga caggttatcc cagtcagaga
    12721 gcagcacttt cgctttggcc ccgctgtcga catagacttc ctggcctgcg atccagtctg
    12781 gcagactgga tcctgaccgc tggccaacgg ggagacagta acgctgggca gtcttcacag
    12841 aaacgataca tccctcgttt tcttttgccg gaagtgcccg accatttaca gtataagtca
    12901 gaccttctgg tgctggtgtg agtgttcggg tatccaatac tttgttatta cagactaaaa
    12961 tcgcctgagt gggctgactt tcagtcggca cgttgatatg gaacttgttc atcactgaac
    13021 tgttcacacg attattagcc agtttgaatc gtagctgccc ttctctagta tccacctgca
    13081 gctggcagtc attactggac aggttctcac tgtcatcaga ataagtgaaa ccatacgcac
    13141 cgtacattgc aggatagatg aagctagaca aagtgccttt gggatcgtaa taacccacca
    13201 gtgtcgtcac cggaacgcca aacttctctg gcttacgcgc ttcgcgggta ttcaccacgt
    13261 cactttcttt ccaggtctgc ccatcggaaa caaagctctt cttatatccc ttggaaatta
    13321 tcttttcacc accgttgata aacagatggc tgttataacc agcttcatga tcgagtgtca
    13381 gaatacggcc tttattctcc gcggaagctg caggggcatg aatatctcta gtccagttgc
    13441 cgtccgacat atgtactttg acaataggat attctgccat catctcagcc attttgtttt
    13501 cactgagatc cctgacagaa gcagtaatct gctcaaacct gtcaatggtg tgttcatacg
    13561 gttccatttt atgctcatcg gcattccact tgctgaatcc agtggaggaa aggctatcga
    13621 acacagcttt attttcaaaa aagcgctgga tgatagccga tgaatttggc gtatacattg
    13681 tgaaacggtt tgcgtcggag aaaggactgc ctcccgccat ggcgtcaaag ccaaacgagt
    13741 gaccgtcgaa cggctcctgg cactgctcgt caagacaact tttccggttt gtctgggctg
    13801 gataaaaatt aggtatgaaa cgttttttat cgctatccca gccccaagtc gagttgatct
    13861 ggtcagcgct gcgatgcacg gaccccctga agccacccac ataatgacca agaccgaagt
    13921 tatgcccgac ttcatggctg aattcattac ccaaggtaga gtctagggta acaattcccc
    13981 ctccaccgga accaccgtgc acctgtactc cattggagta gttaccgcgg tttgtatgtg
    14041 cggtcagttg cgcagccaca taaggatggc tgctttctcc tgaaccagca gtgctgttaa
    14101 taccatagtt ggcattatca ataccatgag agatcagctc cttaccgata ctctgtcgca
    14161 tcgtgccgct atgccatcca ccctcaccgg gatctgcatc tgtcagcaat gtaccggtag
    14221 gtagcatgac ttccttcaga tgaagtggag cataattgtt gacgatcata cgactgaccg
    14281 ggatcgtctg gaaatattcc cgatgcgcct ctttgtcctt ggcaaaatca aagcgccccc
    14341 ggggagtggt cagcatcccg atatcgatgg tatgcaacaa cagctcccct ggtgctccaa
    14401 cattgatgtc atttaggcta ccactcaggt taccttgttt aatcaccaag tttaacccgg
    14461 gcacgatcca gtgcgcaggc agttcagcac tccaggtatg ctgagcataa gcgattcggt
    14521 tattctccag ctcaccggag cggaaccact gaccatttac atatttgaac agcagggtgt
    14581 tacccaccga gagcgtgacc tttcgatctc cataaaaaac cgttgaatta tagccggcat
    14641 acgaaacaaa gcgaaccatc ttaccttcaa gctcggctcc ctgcggcaaa tacatgtctc
    14701 ttatccaacg accatttgct gtctggatct ccaccagtgc attatttgcc agatagcttt
    14761 taatagaact cccgctggca tcactgagtt tgtttacttc agcaaccgta ttaatgatct
    14821 ttttcgttcc attctgagga gtaaaatcga taccatcagc aggaacacca tctaggtggt
    14881 agacggtatc cggtagtgag gagggaggcg aaagggttaa cgtaccgaga attttatcat
    14941 tagcatctcg ggcttccacc tgaacaggtg ttttatcatc agccttcacc agacggacca
    15001 acagcaaact tttccgcagg ctggtcagat gttgttgact ttccccttct ttaggatggg
    15061 cagataaaat ctggctctgt gcaaatttca cctcagcggc cagtcccccc tgcagatcat
    15121 ttacgggctg ggtggtattg aaataaatgg ctgaattatt atcagcagca gttgcagcaa
    15181 atgctcccat cgccagagag gcaaggatca tgcatgacag atactttaat ttcataagtg
    15241 tactccatct ttcattttag atttcatgca gccggatata atagaagtgt taaattttat
    15301 tttttcagga gtgattcact cattcaccag tgttgatttt tataacacac ttatatctac
    15361 ttcagaaaga taacctgata aaaaatatta actccgatca cattcttcta tatattccat
    15421 cacaatattt tgtttaaaaa atgttaaatt cactatcttc gtgataatta acaaacaggg
    15481 tacaaaacca gtaactacaa aatacaataa aaaaaataca tatcgcattt tatcgaacat
    15541 agtgaacatt agctattgct atattttatt tttttgtatt aatctcattt aaaaaaacaa
    15601 ccacaacgcg aaaacaataa ataaataaca aataaaactc atttttatta aaataaaaaa
    15661 caaaagaata tatccttaat cacaatatcc agatgctcct ccccacgtaa tatagacaca
    15721 ggcccaagcg aggttctggt tttcaaattg ttccggacag agaccgccac accaactgtg
    15781 ccgtcgtcac cgattgtaat cacattcgat c
//
LOCUS       DAZFXT010000108        15496 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.108, whole genome shotgun sequence.
ACCESSION   DAZFXT010000108 DAZFXT010000000
VERSION     DAZFXT010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15496
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.108"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            159..482
                     /gene="iraM"
                     /locus_tag="ACHKW0_003659"
     CDS             159..482
                     /gene="iraM"
                     /locus_tag="ACHKW0_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HFO7476423.1"
                     /translation="MKWIVIDTIIQPSCGISFSAIWGNMKMIIWYQSTIFLPPGSIFT
                     PVKSGIILKDKEYPITIYNIAPFNKDLWSLLKSSQECPPGERKITNKCLHNSCIIKIC
                     PYGLK"
     gene            complement(585..1835)
                     /gene="icd"
                     /locus_tag="ACHKW0_003660"
     CDS             complement(585..1835)
                     /gene="icd"
                     /locus_tag="ACHKW0_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HFO7476424.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            2007..2660
                     /gene="rluE"
                     /locus_tag="ACHKW0_003661"
     CDS             2007..2660
                     /gene="rluE"
                     /locus_tag="ACHKW0_003661"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HFO7476425.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2670..3131
                     /gene="nudJ"
                     /locus_tag="ACHKW0_003662"
     CDS             2670..3131
                     /gene="nudJ"
                     /locus_tag="ACHKW0_003662"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HFO7476426.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            3185..4291
                     /gene="mnmA"
                     /locus_tag="ACHKW0_003663"
                     /gene_synonym="trmU"
     CDS             3185..4291
                     /gene="mnmA"
                     /locus_tag="ACHKW0_003663"
                     /gene_synonym="trmU"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)(34)-
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HFO7476427.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            4327..4968
                     /gene="hflD"
                     /locus_tag="ACHKW0_003664"
     CDS             4327..4968
                     /gene="hflD"
                     /locus_tag="ACHKW0_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HFO7476428.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4972..6342
                     /gene="purB"
                     /locus_tag="ACHKW0_003665"
     CDS             4972..6342
                     /gene="purB"
                     /locus_tag="ACHKW0_003665"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HFO7476429.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6510..7181
                     /gene="phoP"
                     /locus_tag="ACHKW0_003666"
     CDS             6510..7181
                     /gene="phoP"
                     /locus_tag="ACHKW0_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HFO7476430.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            7181..8641
                     /gene="phoQ"
                     /locus_tag="ACHKW0_003667"
     CDS             7181..8641
                     /gene="phoQ"
                     /locus_tag="ACHKW0_003667"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HFO7476431.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTFVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8717..9838
                     /gene="roxA"
                     /locus_tag="ACHKW0_003668"
                     /gene_synonym="ycfD"
     CDS             8717..9838
                     /gene="roxA"
                     /locus_tag="ACHKW0_003668"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HFO7476432.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGEVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9984..11213)
                     /gene="pepT"
                     /locus_tag="ACHKW0_003669"
     CDS             complement(9984..11213)
                     /gene="pepT"
                     /locus_tag="ACHKW0_003669"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HFO7476433.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHIID
                     IAQQAMRDCNIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRKS"
     gene            11463..12599
                     /gene="potA"
                     /locus_tag="ACHKW0_003670"
     CDS             11463..12599
                     /gene="potA"
                     /locus_tag="ACHKW0_003670"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HFO7476434.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            12583..13446
                     /gene="potB"
                     /locus_tag="ACHKW0_003671"
     CDS             12583..13446
                     /gene="potB"
                     /locus_tag="ACHKW0_003671"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HFO7476435.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKATEIDD"
     gene            13611..13694
                     /locus_tag="ACHKW0_003672"
                     /pseudo
     CDS             13611..13694
                     /locus_tag="ACHKW0_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400519.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(13720..14310)
                     /locus_tag="ACHKW0_003673"
     CDS             complement(13720..14310)
                     /locus_tag="ACHKW0_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001132166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bfpT-regulated chaperone"
                     /protein_id="HFO7476436.1"
                     /translation="MPMNTTGTSFSSFGISCHRENSFRNSFRGKNDEVIKCSMGERTI
                     RFSVRKFSGNILDTVSRQSTKDINGWIKDERIVYPSRVINQEIDNYCFQKNAKISTEE
                     RQRVFSLVSQEYQLTLDVKAAQSSINHVIMGNASFGKKIDALCDSMSRDVKNRTADSI
                     ANLLADKFYQKHIEPDIDIVKLRNEIPDYLRCAIQA"
     gene            14493..15143
                     /locus_tag="ACHKW0_003674"
     CDS             14493..15143
                     /locus_tag="ACHKW0_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO7476437.1"
                     /translation="MPVILNFSNGSVLPENELEALRHIARSNQNDTITIGGRNMRLHY
                     IQFMDGFSVEPIPGGLWDHLGARESHHLADSLARQLNGGNTFLQAYSLYLEQRQAAPL
                     VQESVIKTLLDRINSNAFPVSLQDFSCTEEHLNCPITLHIPETGVFVRNARNSEICAL
                     YDQEALTELILRNALHPLSRDPFAPEMIISKDKCHFNITKQCFYALPIYPLQQNSI"
     gene            15218..>15496
                     /gene="espW"
                     /locus_tag="ACHKW0_003675"
     CDS             15218..>15496
                     /gene="espW"
                     /locus_tag="ACHKW0_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO7476438.1"
                     /translation="MPKISSVVSSCYHLFSEHQQLSNETTMTNSVSRRIVHKEYGISL
                     KSVPVWLATAKTPLALLNGRHTRSHSFIIAGTPGMGSRSGAQYYAINSD"
BASE COUNT         3984 a         3812 c         3712 g         3988 t
ORIGIN      
        1 gtttaacatc cattgagatt ccttgcttta aattttgttt tatataagca atcgttttaa
       61 ttaatttatt ttttgaaggg gtaatatact catatgcaaa ataaagaaat gaacatccaa
      121 atgaactata ttaaataccg tgggataaga cataacaaat gaagtggata gtaattgaca
      181 cgataattca accttcatgc ggcatatctt tttcagccat atggggtaat atgaaaatga
      241 tcatctggta tcaatctact atatttctcc ctcctggcag tatatttaca ccggttaaat
      301 ctggaattat ccttaaagat aaagaatatc ctattactat ttataacatc gcaccattca
      361 acaaggattt atggagtcta ctcaaaagca gtcaagagtg tcctccagga gaaagaaaaa
      421 taacaaataa atgtttgcat aatagttgca ttataaaaat atgcccatat gggctcaagt
      481 aaagggtttt atataaatat gctcgatatg ctattaataa tacctacata aataaaacaa
      541 cgggcgtgtt atacgcccgt tgcaatatta aacacatgta gtgattacat gttcttgatg
      601 atcgcatcac caaattctga acatttcagc agtttagcgc cttccatcag acgctcgaag
      661 tcataggtta cggtcttcgc attgattgcg ccttccatac ctttaacaat caggtctgcg
      721 gcttcagtcc agcccatatg gcgtaacatc atctcagcgg agagaataat agagccaggg
      781 tttactttgt cctgaccggc gtatttcggc gcagtaccgt gggtggcttc aaacagggcg
      841 cattcgtcac cgatgtttgc accaggggcg ataccgatac cgccaacctg cgctgccagg
      901 gcgtcagaaa tgtagtcacc gttcaggttc atacaggcga taacatcata ttcagccgga
      961 cgcagcagga tctgttgcag gaatgcatca gcaatcacgt ctttaatgac gatctctttg
     1021 ccggtgttcg ggtttttaac tttcagccac gggccgccgt cgatcagttc accgccaaac
     1081 tcttcacgcg ccagctggta gccccagtct ttaaacgcgc cttcggtgaa cttcatgatg
     1141 ttgcctttgt gcaccagggt cacagagtca cggtcgttgg caattgcgta ttcaatcgcg
     1201 gcacggacca gacgtttggt gccttcttca gaacacggct tgataccgat accgcaatgt
     1261 tccgggaagc gaattttctt cacgcccatc tcttcacgca ggaatttaat cactttctcg
     1321 gcgtcggcag agtctgcttt ccattcgata cccgcataaa tgtcttccga gttttcacgg
     1381 aagataacca tatcggtcag ttcagggtgt ttaaccgggc ttggagtgcc ttgatagtaa
     1441 cgtaccggac gcaggcagat gtagagatcc agttcctggc gcagggcaac gttcagagag
     1501 cgaataccgc caccaacagg agtggtcagc ggacctttaa tagcaacgcg atattcacga
     1561 atcagatcaa gggtttcagc aggcagccag acatcctgac cataaacctg tgtggatttt
     1621 tcaccggtgt aaatttccat ccaggagatt ttacgctcgc ctttataggc tttctcgact
     1681 gcagcgtcga ccactttcag catggctggg gttacatcta caccgattcc atcaccttca
     1741 atgtaaggga taatcggatt ttcaggaacg ttgagtttgc cgttttgcag ggtgatcttc
     1801 ttgccttgtg ccggaacaac tactttactt tccattcacc tctccttcga gcgctactgg
     1861 tttgctcgtc tgccaccacg ttgcatatgc gtttgcgtcc tgcgatacgg atgctttaga
     1921 gcaatttttt gttaatgatt tgtaattggc ttgtcaatac taccctattg tttgccgtca
     1981 tgaaagatgc gcgttattag gctataatgc ggcaattcat aatctctgaa aataccatgc
     2041 aaaaaacttc ttttagaaat caccaggtta agcgattcag ctcgcaacgt tctaccaggc
     2101 gtaaacctga aaaccagccc acgcgtgtga tcctgttcaa taaaccctac gatgttcttc
     2161 cgcagttcac cgatgaagcc ggacgcaaaa cattaaaaga attcatcccg gttcagggcg
     2221 tttatgcagc aggtcgtctt gaccgcgata gcgaagggtt actggtgctg accaataacg
     2281 gcgcgttgca ggcgcgttta acccagccgg gtaaacgcac cgggaaaatc tattatgtgc
     2341 aggtggaagg tattcccaca caagacgcac ttgaagcctt gcgcaatggc gtaaccttaa
     2401 atgatggccc taccctgccc gccggcgcgg agctggttga cgaaccagcg tggttatggc
     2461 cgcggaatcc accaattcgt gaacgcaaaa gtattcccac cagctggctg aagatcacct
     2521 tatatgaagg acgtaatcgc caggtgcgcc gcatgaccgc ccatgttggt ttccccacgc
     2581 tgcgactgat tcgctatgcg atgggagatt actctttgga taatcttgcc aatggcgaat
     2641 ggcgagaagt gacagattaa ggatgcaaaa tgtttaaacc gcacgttacc gttgcttgcg
     2701 tggtgcacgc agaaggcaaa tttttagtcg ttgaagagac gattaatggt aaagcgttat
     2761 ggaaccaacc tgccgggcat ctggaagccg atgaaacctt agtggaagcc gccgcccgtg
     2821 aactgtggga agaaaccggc atcagcgcgc agccgcaaca ctttattcgt atgcatcagt
     2881 ggattgcgcc agataaaacg ccgtttttgc gtttcctctt tgccattgag cttgagcaaa
     2941 tatgcccgac ccagcctcat gacagcgata tcgactgctg tcgttgggtc agcgccgaag
     3001 agattttaaa ggcgtcaaat cttcgttcgc cgctggtggc ggaaagtatc cgttgttatc
     3061 aaagcgggca acgctatccg ctggagatga ttggcgattt taactggcct tttacaaagg
     3121 gtgtcatcta aaaggctgcg tgatagaata cgccgccttg aagttcaatg tcgtgagtga
     3181 tccaatgtct gaaaccgcaa aaaaagtaat cgtcggcatg tccggcggtg tcgattcctc
     3241 cgtttctgcc tggctgttgc aacaacaggg atatcaggtt gaaggcctgt ttatgaagaa
     3301 ctgggaagaa gacgacggtg aggaatattg cacagcggca gcggatctgg ctgatgccca
     3361 ggctgtctgc gacaagctcg gcattgaact gcacaccgtt aactttgctg ccgagtactg
     3421 ggacaacgtc ttcgaactgt tccttgccga atataaagcc ggtcgcacgc cgaatccgga
     3481 tattctgtgc aacaaagaga tcaaatttaa agccttcctc gaatttgccg ccgaagattt
     3541 aggtgccgat tatatcgcta ccggtcatta cgtacgtcgg gccgatgtcg atggcaagag
     3601 ccgcctgctg cgtggtctgg acagcaataa agaccagagc tacttccttt atacgctcag
     3661 ccatgagcag attgcgcaaa gcctgttccc ggtcggcgaa ctggaaaaac cgcaggtgcg
     3721 taagattgct gaagatcttg gtctggtcac cgcgaagaaa aaagactcta ccggcatttg
     3781 cttcattggc gaacgtaaat tccgcgagtt cctgggccgt tatctcccgg cgcaaccggg
     3841 caaaatcatt accgtcgatg gcgatgaaat tggtgagcac caggggctga tgtatcacac
     3901 tctcggtcag cgtaaaggtc tgggtatcgg tggcaccaaa gaaggtaccg aagaaccgtg
     3961 gtatgtggtg gacaaagacg tcgaaaacaa cattctggtt gtcgctcagg gccatgaaca
     4021 cccgcggctg atgtctgtcg ggttgattgc ccagcagttg cactgggtcg atcgcgaacc
     4081 attcaccggc actatgcgtt gcacggtaaa aacccgctat cgccagaccg atatcccttg
     4141 caccgtcaag gcgctggacg atgatcgcat tgaagtgatt ttcgatgaac cggttgccgc
     4201 cgtgacgccg ggccagtctg ccgtcttcta taacggtgaa gtgtgcctcg gtggcggtat
     4261 tattgagcag cgtctgccgc tgccggtctg attattatct ttacttaaaa caaggaagca
     4321 gtgaacgtgg caaagaatta ctatgacatc accctcgccc tggccggtat ttgtcagtcg
     4381 gcacgcctag tgcaacaact cgctcaccag gggcattgtg atgccgatgc gctacacgtc
     4441 tcactcaaca gtattattga tatgaacccc agctcgacgc tggcggtttt tggcggtagc
     4501 gaagccaacc tgcgcgtcgg gctggaaacc ctgctcggcg tgctcaatgc cagcagtcgc
     4561 cagggcttaa acgccgaatt aacccgctac acactcagct tgatggtgct tgagcgcaaa
     4621 ctctcctcag cgaaaggcgc gctcgacact ctgggcaacc ggatcaacgg cctgcaacgc
     4681 cagctcgaac acttcgattt acagtccgaa acgctgatga gcgcgatggc tgctatctat
     4741 gttgatgtga tcagcccgct tggcccgcgc attcaggtca ccggttcccc tgctgtactg
     4801 caaagcccac aagtgcaggc gaaagttcgc gcaaccctgc tggcaggcat tcgcgccgcc
     4861 gtgctctggc accaggtcgg cggcggacgt ctgcaactga tgttttctcg taatcgcctg
     4921 accactcagg caaaacaaat tcttgctcat ttaaccccgg agttgtgatc tatggaatta
     4981 tcctcactga ccgccgtttc ccctgtcgat ggacgctacg gcgataaagt cagcgcgctg
     5041 cgcgggattt tcagcgaata tggtttgctg aaattccgtg tacaagttga agtacgttgg
     5101 ctgcaaaaac tggccgcgca cgcagcgatc aaggaagttc ctgcttttgc tgccgacgca
     5161 atcggttacc ttgatgcaat tgtcgccaat ttcagcgaag aagatgccgc acgcatcaaa
     5221 accatcgagc gtaccactaa ccacgacgtt aaagcggttg agtatttcct gaaagaaaaa
     5281 gtggcggaga tcccggaact gcacgcggtt tctgaattca tccactttgc ctgtacttcg
     5341 gaagatatca ataacctctc ccacgcatta atgctgaaaa ccgcgcgtga tgaagtgatc
     5401 ctgccgtact ggcgtcaact gattgatggc attaaagatc tcgccgttca gtaccgcgat
     5461 atcccgctgc tgtctcgtac ccacggtcag ccagccacgc cgtcaaccat cggtaaagag
     5521 atggcaaacg tcgcctaccg tatggagcgc cagtaccgcc agcttaatca ggtggagatc
     5581 ctcggcaaaa tcaacggcgc ggtcggtaac tataacgccc atatcgccgc ttacccggaa
     5641 gttgactggc atcagttcag cgaagagttc gtcacctcgc tgggtattca gtggaacccg
     5701 tacaccaccc agatcgaacc gcacgactac attgccgaat tgtttgattg cgttgcgcgc
     5761 ttcaacacca ttctgatcga ctttgaccgt gacgtctggg gttatatcgc ccttaaccac
     5821 ttcaaacaga aaaccattgc tggtgagatt ggttcttcca ccatgccgca taaagttaac
     5881 ccgattgact tcgaaaactc cgaagggaat ctgggccttt ccaacgcggt attgcagcat
     5941 ctggcaagca aactgccggt ttcccgctgg cagcgtgacc tgaccgactc caccgtgctg
     6001 cgtaacctcg gcgtgggtat cggttatgcg ctgattgcgt atcaatccac cctgaaaggc
     6061 gtgagcaaac tggaagtaaa ccgtgaccat ctgctggatg agctggatca caactgggaa
     6121 gtgctggctg agccaatcca gacagttatg cgtcgctatg gcatcgaaaa accgtacgag
     6181 aagctgaaag agctgactcg cggtaagcgc gttgacgccg aaggcatgaa gcagtttatc
     6241 gatggtctgg cgttgccaga agaagagaaa gcccgcctga aagcgatgac gccggctaac
     6301 tatattggtc gagctatcac catggttgat gagctgaaat aaactcgtat cagtgccgga
     6361 tggcgatgct gtccggcctg tttattaaaa ttatccactt tttctttttt cactttacct
     6421 cccctccccg ctggtttatt taatgtttac ccccataaac acataatcgc gttacactat
     6481 tttaataatt aagacaggga gaaataaaaa tgcgcgtact ggttgttgaa gacaatgcgt
     6541 tgttacgtca ccaccttaaa gttcagattc aggatgctgg tcatcaggtc gatgacgcag
     6601 aagatgccaa agaagccgat tattatctca atgaacattt accggatatt gcgattgtcg
     6661 atctcggatt gcctgacgag gacggtctgt cactgattcg ccgctggcgt agcaatgatg
     6721 tttcactgcc gattctggta ttaaccgccc gtgaaagctg gcaggacaaa gtcgaagtat
     6781 taagtgccgg tgctgatgat tacgtgacta aaccgtttca tattgaagag gtgatggcgc
     6841 gaatgcaggc attaatgcgg cgtaatagcg gcctggcttc acaggtcatt tcgctacccc
     6901 cgtttcaggt tgatctctct cgccgtgaat tatctattaa tgacgaagtg atcaaactga
     6961 ccgcgttcga atacaccatt atggaaacgt tgatacgcaa taatggcaaa gtggtgagca
     7021 aagattcgtt aatgctccaa ctctatccgg atgcggagct gcgggaaagc cataccattg
     7081 atgtactgat gggacgtctg cgcaaaaaaa ttcaggcaca atatccgcaa gaagtgatta
     7141 ctaccgttcg cggccagggc tatttgttcg aattgcgctg atgaaaaaat tactgcgtct
     7201 ttttttcccg ctctcactgc gggtacgttt tctgttggca acggctgcgg tagtactggt
     7261 gctttcgctt gcctacggaa tggtcgcgct gatcggttat agcgtcagtt tcgataaaac
     7321 tacgtttcgg ctgttacgtg gcgagagcaa tctgttctat acccttgcga agtgggaaaa
     7381 caataagttg catgtcgagt tacccgaaaa tatcgacaag caaagcccca ccatgacgct
     7441 aatttatgat gagaacgggc agcttttatg ggcgcaacgt gacgtgccct ggctgatgaa
     7501 gatgatccag cctgactggc tgaaatcgaa tggttttcat gaaattgaag cggatgttaa
     7561 cgataccagc ctcttgctaa gtggagatca ttcgatacag caacagttgc aggaagtgcg
     7621 ggaagatgat gacgacgcgg agatgaccca ctcggtggca gtaaacgtct acccggcaac
     7681 atcgcggatg ccaaaattaa cctttgtggt ggtggatacc attccggtgg agctaaaaag
     7741 ttcctatatg gtctggagct ggtttatcta tgtgctctca gccaatctgc tgttagtgat
     7801 cccgctgctg tgggtcgccg cctggtggag tttacgcccc atcgaagccc tggcaaaaga
     7861 agtccgcgaa ctagaagaac ataaccgcga attgctcaat ccagccacaa cgcgagaact
     7921 gaccagtctg gtacgaaacc tgaaccgatt gttaaaaagt gaacgcgaac gttacgacaa
     7981 ataccgtacg acgctcaccg acctgaccca tagtctgaaa acgccactgg cggtgctgca
     8041 aagtacgctg cgttctctgc gtagtgaaaa gatgagcgtc agtgatgctg agccggtaat
     8101 gctggagcaa atcagccgca tttcacagca aattggctac tacctgcatc gtgccagtat
     8161 gcgcggcggg acattgctca gccgcgagct gcatccggtc gccccactgc tggacaatct
     8221 cacctcagcg ctgaacaaag tgtatcaacg caaaggggtc aatatctctc tcgatatttc
     8281 gccagagatc agctttgtcg gtgagcagaa cgattttgtc gaggtgatgg gcaacgtgct
     8341 ggataatgcc tgtaaatatt gcctcgagtt tgtcgaaatt tctgcaaggc aaaccgacga
     8401 gcatctctat attgtggtcg aggatgatgg ccccggtatt ccattaagca agcgagaggt
     8461 cattttcgac cgtggtcaac gggttgatac tttacgccct gggcaaggtg tagggctggc
     8521 ggtagcccgc gaaatcaccg agcaatatga gggtaaaatc gtcgccggag agagcatgct
     8581 gggcggtgcg cggatggagg tgatttttgg tcgccagcat tctgcgccga aagatgaata
     8641 aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg cagataccag
     8701 cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt ttcttgaacg
     8761 tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta ttgacccgat
     8821 ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc gactggtcag
     8881 tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg atcatctcgg
     8941 tgaaaccaac tggtcattac tggtacaggc agtgaaccac tggcatgagc cgaccgccgc
     9001 gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga tgatctcttt
     9061 ttctgtaccg ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt ttatcattca
     9121 gggtaccggt cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac agcactgtcc
     9181 gcacccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag agctggagcc
     9241 tggcgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc tggaaaatgc
     9301 gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta gcggatttgc
     9361 cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg atgttccacc
     9421 tcgcgctcat cctgcggacg ttctgccgca agagatggat aaactgcgtg agatgatgct
     9481 cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta tatcccagtc
     9541 acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg aaatctacga
     9601 tgcgctgaaa caaggtgaag tgctggtgcg cctgggtggt ctgcgcgtat tgcgcattgg
     9661 cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg cactggatgc
     9721 actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg aagatccgtc
     9781 attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg aagggtaagt
     9841 ttggatttaa gccggatgcg gcgctgattg cgccttatcg tgcctacatg cactgaacgc
     9901 ctgtaggcac gataagtatg ttgaccagta tcaatataac ggtttcatcc cggtaattgg
     9961 cagtccacat acgcatcggg caatcacgac ttccgttgcg ccgttaactc ggcaatacgg
    10021 acaatcacct gcaccgcttt ttccatacct tccagagtca caaactcatg cttaccatga
    10081 tagttgtaac cgccagtgaa caggttcggg cacggtaatc ccataaacga caactgcgcg
    10141 ccgtcggtac cgccgcggat cggtttcagt tccggttcaa tattgcagtc acgcatcgcc
    10201 tgctgggcga tatcgataat atgcggatgc tcaaccactt tctcgcgcat attgtagtaa
    10261 ctgtcttcaa tcaccagttc aatgtagcaa tcaggatgta accctttgcc cacttttttg
    10321 gcgatctcca tcattttacg tttacgcgct tcaaactgtt tacggtcgaa atcacggatg
    10381 atgtagtgca tatcggctcg gtcaacggtg cctttcatgc tcgccaggtg atagaaacct
    10441 tcatagcctt ctgtcatttc cgggctttca tccgccggaa cttccgcatg aatacgtgcc
    10501 gccagcgaca gcgcatttac catcactcct ttcgccgtgc ccggatgaac attgttaccg
    10561 acaattttga tattgaccga tgcggcgttg aagttttcaa actccagttc acctacgcca
    10621 ccaccgtcaa cagtgtaagc ccagcgggca tcgaaggcat caacatcaaa atgtttcgcc
    10681 cctttgccca cttcttcatc cggggtaaag gcgacgcgaa tatcaccatg cggaatgttt
    10741 ttctgttgca ataccgccag cgcggtcatg atttctgcaa taccggcttt gtcatcggca
    10801 cctaacaagg ttttaccatc ggtagtaatc agcgtctgac ccagtagctg atgcagcacc
    10861 gggaacataa ccggtgataa aacttcatcg ccgataccca gcgcaatatc gccaccgcga
    10921 tagttttcaa caatttgcgg attcacattt ttgccgctgc aatccggtga ggtatccaca
    10981 tgagaaataa agccaatcgc cgggatatcg ccagggacgt tagccggtaa cgtcgccatc
    11041 aaagtgccct tctcacttaa ggtcacattg ataagcccca tctcttcgag ctgctctttc
    11101 agcagatgca ataacttcca ttggccttcc gtgctgggaa cctgtctcac ccctgctttt
    11161 gattgggtat ccagagacac gtagttcaaa aatcgctcaa gtagtttatc catgtagtca
    11221 ccctcacttt ttgtgacaac attattaaga agcaagaaaa gacaaatatt gcgtcaggtc
    11281 acttttaccc ctgcaagcgg gaatatttat cagcattacc ttaatgaata taaagctaag
    11341 ccagtaattc acaacaaaga ttggcgattc aagcggtttg ccgtagaatt accgccctca
    11401 ttaagagtca ccaaggtggt taaccacaaa ccccgcatcg gtaagccatc cgttgcgttt
    11461 acatgggaca gagtaaaaaa ttgaataaac aaccgagttc gctttcaccg ctggtgcaat
    11521 tggcgggaat tcgcaaatgc tttgatggta aagaggtcat tccccagctg gatctgacta
    11581 tcaacaatgg cgagttcctc acgctgcttg gcccttctgg ctgcggtaaa acaaccgttc
    11641 ttcgcctgat tgcaggtctg gaaactgttg attccggacg catcatgctg gataacgagg
    11701 acatcaccca cgttccggcg gaaaaccgct atgtgaacac tgttttccaa agctacgcac
    11761 ttttccccca catgaccgtg ttcgaaaatg tggcctttgg gttgcgcatg caaaaaaccc
    11821 ccgctgctga aattacgccc cgcgtgatgg aagccctgcg gatggtgcaa ctggaaacct
    11881 tcgctcaacg taaaccgcat cagctctctg gtggtcaaca gcaacgcgtc gccattgccc
    11941 gcgcggtggt taacaagcct cgtctgttgt tgctggatga gtcgctctca gcgctggatt
    12001 acaaactgcg caagcaaatg cagaacgagc tgaaagcgtt acagcgtaag cttggcatta
    12061 cattcgtctt cgtgactcac gaccaggaag aagcactcac catgtcagac aggattgtgg
    12121 tgatgcgcga tggtcgcatt gagcaagacg gcacgccgcg tgaaatctac gaagagccga
    12181 aaaacctgtt tgttgccggg ttcattggcg aaattaatat gtttaacgcc acggtgatcg
    12241 agcgactcga cgagcagcgc gtacgcgcca acgttgaagg ccgcgaatgt aatatctacg
    12301 ttaacttcgc cgttgaaccg gggcaaaaac tgcatgttct gctgcgcccg gaagacttac
    12361 gtgttgaaga gattaacgac gacaaccacg ctgaagggct gattggttac gttcgtgagc
    12421 gtaactacaa aggcatgacg ctggagtcgg ttgttgaact ggaaaatggc aagatggtga
    12481 tggtcagcga attcttcaat gaagacgacc ctgactttga ccactctctc gaccaaaaaa
    12541 tggccattaa ttgggtagaa agctgggagg tcgtactggc tgatgaagaa cacaagtaag
    12601 ttccagaatg tagtgattgt cactattgtc ggttggcttg tgttgtttgt ctttctgccc
    12661 aacctgatga tcattggcac cagctttttg acccgcgacg acgccagttt cgtcaaaatg
    12721 gtctttacgc tggataacta cacgcgtctg ctcgatccgc tctattttga agtgctattg
    12781 cactcgctga atatggcgct gatcgccacc ctcgcctgcc tggtgctggg ctacccgttt
    12841 gcctggtttc tggcgaagct gccacacaag gtgcgtccgc tgctgctgtt tctgctgatt
    12901 gttcccttct ggaccaactc attgattcgt atctacgggc tgaaaatttt cctcagcacc
    12961 aaaggctatc tcaacgagtt tttgctctgg ctgggcgtta tcgacacacc aatccgtatc
    13021 atgttcacgc ccagtgcggt gattatcggt ctggtttaca ttctgctgcc gtttatggtg
    13081 atgccgctgt actccagtat cgaaaaactg gataagcctt tgcttgaggc ggcgcgcgat
    13141 ctcggtgcca gcaagttgca gacctttatc cgcatcatta tcccactgac gatgccgggg
    13201 attattgccg gatgtctgct ggtgatgctg ccagcgatgg gcctgttcta tgtgtccgac
    13261 ctgatgggcg gtgcgaaaaa cctgctgatc ggtaacgtca tcaaggtaca gttcctcaat
    13321 attcgtgact ggccatttgg tgcagccacc agcattacgc tgactatcgt aatgggcctg
    13381 atgttgctgg tttactggcg cgcttctcgt ctgctgaata agaaggcaac agaaatagac
    13441 gattagtgtt ttatattcaa aaggttaata gcaaatttgg catgcttttt actgtcgagt
    13501 aaactgcatg cctctatcca taaaatcaaa cagttgcaat ctaattttgg agaacgtttt
    13561 ttcttcaccc ttttagcaaa aagctataaa aaacaacaaa tggtataaaa atgaacaatc
    13621 ctaatccatg catgacgtat ggcgcctgtt gtgcattttt ccgcgtctct ttttaccaga
    13681 gcaaaaccaa tgatactgac ctagtcgtct cccagagact tatgcctgta tagcacacct
    13741 caaataatct gggatctcat ttcgtagttt tacaatatca atatccggct cgatatgttt
    13801 ctggtaaaac ttgtctgcaa gcaagtttgc tatgctatct gccgtacgat tttttacatc
    13861 ccggctcata ctatcacaaa gagcatctat tttcttgcca aaagaagcat tccccattat
    13921 cacatgatta atagaacttt gtgctgcctt aacatcaaga gttagttgat attcctgact
    13981 cacaagagaa aagacccttt gcctctcttc agtggaaatt tttgcatttt tttgaaaaca
    14041 gtaattatca atctcctggt tgatcaccct tgagggatat actatccgtt catcttttat
    14101 ccacccatta atatctttag tactctgcct actcactgta tccagtatat tgccgctaaa
    14161 tttgcgaaca gaaaagcgaa tcgtccgttc tcccattgaa cattttatga cctcatcatt
    14221 cttccctcgg aaagagttcc tgaaggagtt ttctctatgg caacttatac caaaagagct
    14281 gaaagacgta cctgtagtat tcatcggcat tataattcct ttttataaaa aagtgtttac
    14341 aatattgcat acttgtgttt attgtagcca gaaaaaacat gcatgcaata atattctatt
    14401 aaaaattgta ttgttttatt cacatatcat gagcggtatg ttgactcttg ttaccaatat
    14461 agttcacaaa atgggttgaa aaggataata atatgccagt catattaaat ttttctaatg
    14521 gaagtgtatt gccggaaaat gagctggagg ctttacgaca tattgcaaga agcaatcaga
    14581 atgacactat tactatagga ggccgtaata tgaggcttca ttatatccag tttatggatg
    14641 gttttagtgt agagcctatt cctggtggac tttgggacca tcttggggca agagagtctc
    14701 atcatctcgc agacagcctg gcaagacaac ttaacggagg taacactttt ctgcaggcat
    14761 acagcttata tctggagcag aggcaagctg ccccgcttgt gcaggaaagc gtcataaaaa
    14821 cactactaga tcgaataaat tcgaatgcat ttcccgttag tttacaagac ttttcctgta
    14881 ctgaggagca tcttaattgt ccgataacgc tacatattcc tgagacaggt gtttttgtca
    14941 gaaatgctcg aaattcagaa atatgtgcat tatatgatca ggaagcattg actgaactta
    15001 tcctgcgtaa cgctctccac cccctcagcc gtgacccctt tgctccagaa atgattataa
    15061 gcaaagacaa gtgtcatttt aatataacaa aacaatgttt ttacgcatta cccatatacc
    15121 cccttcaaca aaacagtatt taaaaataaa aaaacaatat aagaagcata caataagtta
    15181 atcaccatat aacaaacata tagcgaggag attatttatg cccaaaatat catcagttgt
    15241 atcatcatgt taccatctgt tcagtgaaca tcaacaactt tcaaatgaaa caacaatgac
    15301 gaactccgtc tccagaagaa ttgttcataa agaatatggt atatctttaa aatccgttcc
    15361 tgtatggttg gctacagcta aaactcccct tgctctactc aatggcagac atacaagaag
    15421 tcactcattt attattgcag ggactccagg aatgggaagc aggagcggag cccaatacta
    15481 tgccataaac agtgat
//
LOCUS       DAZFXT010000109        15420 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.109, whole genome shotgun sequence.
ACCESSION   DAZFXT010000109 DAZFXT010000000
VERSION     DAZFXT010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.109"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(272..1183)
                     /gene="gsiD"
                     /locus_tag="ACHKW0_003676"
     CDS             complement(272..1183)
                     /gene="gsiD"
                     /locus_tag="ACHKW0_003676"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HFO7476439.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1186..2106)
                     /gene="gsiC"
                     /locus_tag="ACHKW0_003677"
     CDS             complement(1186..2106)
                     /gene="gsiC"
                     /locus_tag="ACHKW0_003677"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HFO7476440.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2124..3662)
                     /gene="gsiB"
                     /locus_tag="ACHKW0_003678"
     CDS             complement(2124..3662)
                     /gene="gsiB"
                     /locus_tag="ACHKW0_003678"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0034634 - glutathione transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034775 - glutathione transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HFO7476441.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGLTYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3682..5553)
                     /gene="gsiA"
                     /locus_tag="ACHKW0_003679"
     CDS             complement(3682..5553)
                     /gene="gsiA"
                     /locus_tag="ACHKW0_003679"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HFO7476442.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5540..6505)
                     /gene="iaaA"
                     /locus_tag="ACHKW0_003680"
     CDS             complement(5540..6505)
                     /gene="iaaA"
                     /locus_tag="ACHKW0_003680"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HFO7476443.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6709..7944
                     /gene="moeA"
                     /locus_tag="ACHKW0_003681"
     CDS             6709..7944
                     /gene="moeA"
                     /locus_tag="ACHKW0_003681"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0032324 - molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HFO7476444.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASNV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7944..8693
                     /gene="moeB"
                     /locus_tag="ACHKW0_003682"
     CDS             7944..8693
                     /gene="moeB"
                     /locus_tag="ACHKW0_003682"
                     /EC_number="2.7.7.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HFO7476445.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDAMTRINPHIA
                     ITPVNALLDDAELAAMIAKHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8769..9431)
                     /gene="fsa"
                     /locus_tag="ACHKW0_003683"
     CDS             complement(8769..9431)
                     /gene="fsa"
                     /locus_tag="ACHKW0_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HFO7476446.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9562..10461
                     /gene="ybiY"
                     /locus_tag="ACHKW0_003684"
     CDS             9562..10461
                     /gene="ybiY"
                     /locus_tag="ACHKW0_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HFO7476447.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10467..12899
                     /gene="ybiW"
                     /locus_tag="ACHKW0_003685"
     CDS             10467..12899
                     /gene="ybiW"
                     /locus_tag="ACHKW0_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HFO7476448.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIEMLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13045..13860
                     /gene="ybiV"
                     /locus_tag="ACHKW0_003686"
     CDS             13045..13860
                     /gene="ybiV"
                     /locus_tag="ACHKW0_003686"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HFO7476449.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPHFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14012..15277
                     /gene="ybiU"
                     /locus_tag="ACHKW0_003687"
     CDS             14012..15277
                     /gene="ybiU"
                     /locus_tag="ACHKW0_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HFO7476450.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3747 a         4252 c         3918 g         3503 t
ORIGIN      
        1 agttaatgat gaaaaaaaga ccaataaatg acagtgctgc caataaaaat aaaattatca
       61 gccttatctt tatcttttcg tataaactca gcatgttaat acatttaaca aaataactat
      121 ctgaacgctg tgccgctgca aaaatagtcc cttgctgcgg ctttttaacc aaattacccg
      181 attctacaaa atcagcagaa agttacaaca cttgcttagg gcaataattt cctgtgaggt
      241 gattaccctt tcacgcaata ttcaaacgta actatccttt aattttcgga tccagcgcat
      301 cgcgtaaacc atcgcccaac aaattgaacg ccagtacggt cagaaaaata gccagggccg
      361 gaaaaacagc gacatgcggc gcgataacca tatccgctcg tgcctcattg agcattgctc
      421 cccactctgg tgtcggcggc tgcgcaccga ggccgagaaa tgacaggctg gcggcagaga
      481 taatcgaggt gccaatgcgc atggtgaaaa acaccacgat agacgagacg gtcccaggca
      541 agatatgacg caataaaatg gtcatatcgc tggcaccaat actgcgtgct gactcaataa
      601 aggtttgctg tttcaacacc agcgtgttgc cgcgcaccag tcgggcaaac gcggggatgg
      661 aaaaaatggc gactgcgata atcacgttag caatgccgct tcccaacacc gcaacaacag
      721 cgatcgccag taaaataccc gggaaggcaa acagcacatc gcaaatgcgc atgatcaacc
      781 gatcccacca gccttcataa tatccagcca acaagcccag caacgtcccg atcgccgcac
      841 cgataaacac ggcaaacacg cccgccgcca gcgagatttg cgcaccaacc aggacacggc
      901 tgaaaatgtc gcgccccagt gaatcgacgc caaaccagtg ctgcaaagaa ggtccgttat
      961 tcaggttgtc ataatcaaaa taattttcgg cgtcataggg agcgatccag cgtgcaaaaa
     1021 tggccaccac aatcaataaa ataacgaata atgcggcggt catcgccata tgctgacggc
     1081 gaaatcgtcg ccagaattca tgccacggtg tacgcacctg gtcaggtttg accagtggca
     1141 tggcgtttaa caccgcctga cgtcgccagt taaatagtcg catccttact tgtacctgat
     1201 agccgggtta atggcggcgt aaagcacatc caccactaag ttgataagaa taaattccag
     1261 cgagaaaagc agaatttccg cctgaatcac cgggtaatcg cgcatttcta cggagtcaac
     1321 gagtaagcgt ccaagtccgg gccagttgaa aactttctcc accacgatag atccaccgag
     1381 caaaaagcca aactgtaagc ccatcatggt cactaccggg atcatcgcgt tgcgtagccc
     1441 gtgtttgagg acaacccagg tttcgctcac ccctttcgcc ctcgcggtac gcatataatc
     1501 ttcgcttaaa acgtcgacaa atgacgcacg ggtaaagcgc gccatcacgg cggccactgc
     1561 cgcgccgagc gtcagggagg gtaaaatgta gtgctgccaa ctgtctgctc ccacagtagg
     1621 cagccagccc agttcaacgg agaatacctg aattaaaagc atccccagag caaatgccgg
     1681 aaacgagatc cccgacaccg caatggtcat actcaatcga tccggccaac ggttacgcca
     1741 gacggcggcg ataattcccg ccgccatacc aaatatcacc gcccagacca tactggttat
     1801 ggtcagccac agcgttggca taaagcggct ggcaatctca tcggcaactg gacgacgcga
     1861 caccatggac aggccaaaat ccccctgcac agcattgctg atatagtgcc agaactggtg
     1921 atacagcggc tgatccaacc ccagctgctg acgcaccagt tctataacct gcgcatcagc
     1981 ttcgggcccg gcaatcaatc gcgccggatc gccgggcagc atatggacaa ataaaaacac
     2041 cagcaccgag acgataaaca gcgtcggaat caaccccagt aatcgtttga taacgtaatt
     2101 aagcattcca ctccctgcgt tgcttattgc aaatccgcat cttcaaagct gaagccggtg
     2161 tctggcatga tccaaaaacc ggtcaggttt ttactgtgtg ccgacaccag tttttctacc
     2221 accagcggga tccacggcga ctcttgccag atgatatcct gcgccgcctt atataaacgg
     2281 gtcttttccg ccggatcatt agttttcagt gcctgagcca ggaagtcatc cacctgttta
     2341 ttgctgtaaa acgcggtatt aaacagcgtc ggtggccagt tttgcgaggc aaacaacggc
     2401 gatagtgccc agtcggcttc gccggttgaa gccgaccagc cagtgtagaa catccgcacg
     2461 ccgctctctt tttgcccttt accttcaact tctgccgccc gctgtccggc atccatcgca
     2521 gtcacctggg ctttaatccc gacctgcgct aactgctgct gggtaaattg cagcactttc
     2581 tgcgcggtgc tgtggttatg tgacgaccac agcgtggtac tgaaaccgtt gggatatccc
     2641 gcctctttca gtaattcgcg cgctttcact ggatcgtaag gccacggttt ataactttgc
     2701 gcgtaggcga tacttggcgg taccacacca gtagctggcg ttgcatagcc cgcaaaggca
     2761 actttcacca gcgccggacg gttaatggcg taattcagcg cctcacggac cttcgggtta
     2821 tcgaacggct tttgcgtcac gttcatactg atataacgct gcataattga cggactggcc
     2881 atcaactcga tatttttgtt tttctccagc agtgtggcct gctcgtaggg aatggggaaa
     2941 gcaaactgcg cttcaccggt ttgcagcatt gccgcgcggg tgttgttatc cgccaccgga
     3001 cgccaggtta tgctgtccag tttgggcaat cctggctgcc agtaacccgc gaattttttc
     3061 accttcacaa aatcggtctg attccaggta tccagttcat acggtccggt tcccaccgga
     3121 tgaaaaccaa tctccttgcc atatttttcc agcgctgccg gtgaaatcat cgcggtcgcc
     3181 ggatgggcaa gaatattaat aaacgctgag aacggctgtt tgagagtaat ctttactgtt
     3241 gttggatcga tcgcctccgt tttggcaata ttcttataca ggttatagcg tttaagatga
     3301 ttcgccggat cgctggcccg gtccagattc gcttttaccg ccgctgcgtt gaaatcggtg
     3361 ccatcctgga atttaattcc ttcccgcaat ttcacggtgt atgtaaggcc gtcatcggaa
     3421 acggtataac tctccgccag cacgtttttc agtttcatct ctttatccag accaaacagc
     3481 ccctggtaaa acgatttcgc tacggcctga gataacgtgt catttgcgtc atacggatcg
     3541 agcgtggtga agttcgatcc taccgccacc accacatctt tggcagcgaa tgcacaagat
     3601 gccatcaacg ctgtcgcaat gcccaacgcc actaacccac tacggtgtac agctcttgcc
     3661 attttattct ccgcctgaat gttatctacg catgaatgcg tattctgatt gtggttgtgc
     3721 gacgtaatgc cccggcccga cgcattgcaa cgagacggct gccacctctt cgccacgcag
     3781 atgaatattg ctgggaagat cgtccgacag cagtacacgc tgcggtcgtt gtcgggacgg
     3841 ttcagcgacc ggaactgccg ccagtaattt acgcgtataa ggatgctgcg ggttttcgaa
     3901 gaccgcgcgc cgtggaccaa tttcaacaat ttgtccgaga tacatcaccg ccacacgatg
     3961 actaatccgc tctaccacgg ccatatcgtg ggagataaac agatacgcaa tgccgaaatc
     4021 acgctggaga tcgagcaaca agttgataat ctgcccgcga atagaaacat ccagcgccga
     4081 aacggcttcg tcggcaatga tcacttttgg attcaatgcc aacgcgcgag caatgcagat
     4141 gcgctggcgc tgaccgccgg aaaactcatg cgggtaacgc caggcatgtt caggtaacag
     4201 gcccacgcgc tccagcaacc acgcaacgcg tgcagccgcg tctttacctg gcaataaacc
     4261 gtgtacacgc agcggttcga taatcgaatc accgatggtc tgacgtgggt ccagcgaagc
     4321 gtaagggtcc tgaaaaataa actgaatatc ccggcgcaat gcctgaagtt tgccgggtga
     4381 caaggtatcg attctctgac cgttaaagat aatttcgccg ccctgcgatt cgaccaggcg
     4441 cagcaacgcc cgcccggtag tggatttacc gctgccagac tcgcccacca gcgatagcgt
     4501 ttcgccaggc cagagatcaa aactgacttt ctcaacggca tgcacttccc gcgttacgcg
     4561 attcaacaaa ccgctgcgca aagggaaacg ggtgacaaga ttacgcactc gtaaaacagg
     4621 ttcgccatcc accaccgttt tctgctcgat ggggggggcc tgtttcgctg gatgttcaag
     4681 cgatatcaac gggaaacgtc ggggataatc taaccctttc atcgcaccaa gttgcggaac
     4741 agcagctaac agcgcacggg tgtaaggatg ttgcggtgca tgaaaaatct gttcgacggt
     4801 acccgtttcc accgcctcgc cctgatacat caccagtacc cgatcggcaa tctctgccac
     4861 cacgcccata tcgtgagtga taaagataac gcccatcgac atctcttttt gcaatacttt
     4921 gattaattgc aggatctgcg cctgaatagt gacatccagc gcggtggttg gctcatcggc
     4981 aatcagcacc gccgggcggc atgacagcgc catcgcaatc atcactcgct ggcgcatccc
     5041 gccagagagt tgatgcggat aacgtgaaag aatggtttgt gcctcaggaa tgcgtacctg
     5101 atccagcatc cgcttcgcct cgaccatcgc ttcttcacga ctggcgttct gatgcagacg
     5161 aattgattcg gcaatctgtt cacccacagt aaataccggg ttcagcgatg tcatcggctc
     5221 ctgaaatatc atcgccatat ccgcaccgcg aacatggcgc atttgtgcag cgctctgctc
     5281 gctaagttca atcacttcgc gactgcgccg ctgcaacagc attttatcgc actgtactaa
     5341 accgcccgcc tgttccaaca ggcgcatcaa tgccaacgca gtcactgact taccggagcc
     5401 ggattcgcca acaattgcca gcgtctcacc gcgttgcaga ctaaaagaga gattgcggac
     5461 cgcagctatt ttctgctggt cctgcataaa ggcaatattc agattttcaa ccgccagcac
     5521 attaccggca tcaagttcat cactgtgtgg caacggtgtc ccctttttca cggtagatac
     5581 cggtggttgg cgtatcgcct gcgtagcccc aggcgcgata cattccttcg gtgttaaacg
     5641 gtagcgcgac attcccttca tggtcgatag cgattaagcc accgctaccg ccaagcgcag
     5701 ggagtttttc cattactacc cgctcgcagg cttccgcgag acttaatccg ccgtaatcca
     5761 ttaacgcggc gatgtcatat gccgccagcg cgcggatgaa gacttcgccc gtgccggtac
     5821 aagaaaccgc cacactggcg ttattggcgt agcatccggc acccactaag ggactatcgc
     5881 caactcgtcc gggtaattta ttggtcattc cgcctgtgga cgtggctgcc gccaaattgc
     5941 cgtctaaatc caacgccacg gcccccacgg tgcccatttt ctgtttttca tccagtggcg
     6001 caccgctatg gtcgaggacg gttgcccctt ccttgcgcgc tgccagtagt tgttcataac
     6061 gcaaaggcgt ggagaaaatc tccggcgaga cgcgctccat gccacgagca aacgcaaaat
     6121 tttctgcccc ttcgccaatc atcatcacat gcgggctttg ctccatcacc agccgggcgg
     6181 caagaaccgg attacgcaga tgactaacgc ccgccaccgc accggctttc agggtgttac
     6241 catccatcac acaggcgtcc agttcatggg tttcatcacg cgtaaagaca gcgccaattc
     6301 cggcgttaaa cagtggacac tcttccagca gacgcaccgc ttccgtcacc acatccagcg
     6361 cactttcgcc cgcttccagc attttctgcc cggtttcaac aatggcagac aacgcctcaa
     6421 tgtagcgtaa ttcctgttgc agactcatct gcgcgcggct aattgcacct gcgccaccat
     6481 gaattgcaat gactgctttg cccatagtat tcgtccatta atggtggctc gccacttttg
     6541 ctataaatat cagaattttt atgaattact cattcaattt ttgaatatag aaagatggat
     6601 atggcatgta aaggcaggag ccaacgctac gcccgaggtg gcatacagtc tgtggcgttt
     6661 ttctgacata ataggcaaat tcgattttgc ctccgcagga gtgttttcat ggaatttacc
     6721 accggattga tgtcgctcga caccgcgctt aatgagatgc tttctcgcgt caccccactg
     6781 accgcccagg aaacgctgcc actggtacag tgttttggtc gtattctggc gagcaatgtc
     6841 gtttcgccgc ttgatgttcc ggggtttgat aactccgcaa tggacggcta cgcggtgcgt
     6901 ttagccgata ttgcctccag gcaaccgctg cccgttgccg gtaaatcctt tgccggtcag
     6961 ccgtaccatg gtgaatggcc tgcgggcacc tgcattcgta ttatgaccgg tgcgccggtg
     7021 ccggaaggct gcgaagcggt ggtgatgcag gagcagactg aacaaacgga caatggcgtg
     7081 cgttttactg ctgaagtgcg tagcgggcaa aatattcgcc gtcgcggtga agatatctct
     7141 gcaggtgcgg ttgttttccc ggcgggaact cgcctgacta ccgcagagct gccagtaatt
     7201 gcttcactgg ggattgccga agttccggtg attcgtaaag tgcgtgtagc gcttttttct
     7261 accggtgatg aactccagtt gcccggtcag ccgctgggcg acggccaaat ctacgacacc
     7321 aaccgtctcg ccgtacacct gatgttagaa cagctgggtt gcgaggtaat caacttaggg
     7381 attatccgcg acgatcccca tgccctgcgc gccgcattta ttgaagccga cagccaggcg
     7441 gatgtagtga tcagttccgg cggtgtttca gtgggtgaag cggattacac caaaacgatt
     7501 cttgaagagc tgggggagat cgccttctgg aagctggcga ttaaaccagg taaaccgttc
     7561 gcgttcggta aactcagcaa tagctggttc tgcggcctgc cgggcaaccc ggtttcagcg
     7621 acgctgacct tctatcaact ggtacagcct ttgctggcaa aactaagcgg caacaccgcc
     7681 agcggcctgc ccgcgcgcca gcgcgtacgc acagcgtccc gcctgaaaaa aacgccagga
     7741 cgtcttgatt tccagcgcgg cgtgctgcaa cgcaacgccg acggtgaact tgaagtgacg
     7801 accaccggac atcagggttc acatatattt agctccttta gtctcggcaa ctgctttatc
     7861 gtgctggaac gcgatcgcgg caatgtggac gtgggcgaat gggtggaagt agaaccgttt
     7921 aacgcgttgt tcggaggcct gtaatggcgg aactcagcga tcaggagatg ctgcgctaca
     7981 accggcaaat cattctgcgc ggttttgatt ttgacggtca ggaggcgcta aaagattctc
     8041 gtgtgctggt agtcggcctc ggtggactcg gctgcgcagc ttcgcagtat ctggcaagcg
     8101 ccggtgtcgg caacctgacg ctgctcgact tcgacacggt ttcgctctcg aatctgcaac
     8161 gccagacgct gcacagtgat gctacggtcg ggcaaccgaa ggtagaatcc gcccgtgacg
     8221 ccatgacgcg gatcaaccca catatcgcga ttacgccagt caatgcactg cttgatgacg
     8281 ctgaacttgc tgcgatgatt gcaaaacacg atctggtact cgactgtacg gataacgttg
     8341 cggtgcgtaa tcaactgaac gctggctgtt ttgccgcgaa ggtgccgctg gtttccggcg
     8401 cggcaattcg tatggaaggg caaatcacgg tcttcactta tcaggacggc gaaccgtgct
     8461 atcgctgcct tagccgtttg tttggcgaga acgcattaac ctgcgtagaa gcaggcgtga
     8521 tggcaccgtt gatcggcgta attggttcgt tgcaggcaat ggaagcgatc aaactgctgg
     8581 caggttatgg caaaccggcc agcgggaaaa tcgtcatgta cgatgcgatg acctgtcagt
     8641 ttcgcgaaat gaaactgatg cgcaatccgg ggtgtgaggt gtgcgggcag taattgccac
     8701 ctccaggcca gataagacgc ggcactccgc cacgtcatca gcaatttatg aggatgcaga
     8761 acgtccggtt aaatcgacgt tctgccaaac gctcccagcc agtcctgttc aaacttcgcc
     8821 acagcggctt ccaccgccgg atagctaatc atctgttgtg ccacatccag tggcagcgta
     8881 attgattcac atcctgccag taagcagtcc agcgcctgac gcggggtttt gaaactcgct
     8941 gccagcactt tcgcctgcgg cgcatgcatt ttcaataact ggtgtaagtc ggtcacagtc
     9001 tgaatgccgc taccgccctg agcatcaata cgattaacgt aaggcgcaac atattccgca
     9061 cctgccagcg ccgacagcag cccttgtgct gcgccatata ccgcggttcc cagcgttgga
     9121 atcccttccg cttttaacat cttaatagct gccagcccct cggcggtcac cggaactttc
     9181 accacgatat ccgcaataat agaacgcaat ttacgtgcgt cattcaccat cccttcagca
     9241 gtggtagcca ttacctgggc aaacagacgc ccctgaccgc ccatcgcttc atgaagttgc
     9301 ggaagcacaa catccagcgg ttttttaccc gcggcgataa tgcttgggtt agtggtcaca
     9361 cccgccagcg gaaaaatacg tgacagcgcc ttcaccgcaa caacgtctga agtatccaga
     9421 tacagttcca taaccatcct caaaatgtac tttttgtctg aatgtagact atcacgggaa
     9481 aactctctga acagttgacg cacatcaatc taactttcat tcgaaagtat tttaatgttt
     9541 gaacgaaaca gagaggcaac tatgattttc aatattcagc gctactcgac ccatgatggc
     9601 cccggtatcc gcacggtcgt atttcttaaa ggctgttcgc tgggctgccg ctggtgtcag
     9661 aacccggaaa gccgcgcccg cacgcaggat ctgctgtatg acgcacgact gtgtctggaa
     9721 ggctgcgagc tgtgcgctaa ggccgcgccg gaagtgattg agcgcgcgct gaatggtttg
     9781 cttattcatc gggaaaagtt aaccccggag catctgacgg cgttaaccga ctgctgtccg
     9841 acacaggcat taaccgtgtg tggtgaagtg aaaagcgttg aggagatcat gacgaccgtt
     9901 ctgcgcgata aaccgtttta cgatcgcagc ggcggcggtt taacgctttc gggtggtgag
     9961 ccctttatgc agccggaaat ggcgatggcg ctactgcaag ccagccacga ggcaggcatt
    10021 catactgcgg tagaaacctg tctgcatgtg ccgtggaaat atatcgcccc ttctctgccc
    10081 tatatcgatc tgtttcttgc cgatttaaaa cacgttgccg acgcgccgtt taaacagtgg
    10141 accgacggta acgccgccag agtgctggat aacctgaaaa aactcgccgc agcgggcaaa
    10201 aaaatcatta tccgcgtgcc gctgattcag ggctttaatg ccgacgaaac ctctgtaaaa
    10261 gccattaccg attttgccgc cgacgagctg cacgttggcg aaattcattt tctgccctac
    10321 cacacgctgg gcatcaacaa atatcactta cttaatctgc cctatgacgc cccggaaaaa
    10381 ccgcttgatg cgccagaact gctcgacttt gcccagcagt atgcctgcca gaaagggtta
    10441 accgcgacct tacgaggata acaaccatga ccacactgaa actggacacg ctcagcgacc
    10501 gcattaaagc gcacaaaaat gcgctggtgc atattgtgaa accgccagtc tgtaccgagc
    10561 gcgcgcagca ctataccgag atgtatcaac aacatctcga taagccgatc ccggtacgtc
    10621 gcgcgctggc actggcgcat cacctggcga atcgcaccat ctggatcaaa cacgatgagt
    10681 tgatcattgg caaccaggca agcgaagttc gcgccgcgcc gatcttcccg gaatatactg
    10741 tctcgtggat cgaaaaagag attgatgatc tggcagatcg tcccggtgct ggctttgcgg
    10801 tgagcgaaga gaacaaacgc gttctgcatg aagtgtgccc gtggtggcgc ggtcagaccg
    10861 tacaggatcg ctgctacggc atgtttaccg atgagcaaaa aggtctgctg gcgaccggaa
    10921 tcattaaagc ggaaggcaat atgacctccg gcgatgcgca cctggcggtg aatttcccgc
    10981 tgctgctgga aaaaggtctt gatggtctgc gtgagaaagt agcggaacgt cgctcgcgca
    11041 tcaatctgac ggtgctggaa gatttacatg gcgaacaatt cctgaaagcg attgatatcg
    11101 tgctggtggc agtcagtgaa cacattgaac gtttcgctgc cctggcgcgt gaaatggctg
    11161 ctaccgaaac tcgcgaaagc cgtcgcgatg aactgctgac gatggcagaa aactgcgatc
    11221 ttatcgccca ccagccgccg cagactttct ggcaggcgct gcaactgtgt tacttcatcc
    11281 aattgatttt gcagatcgaa tctaacggtc actcggtatc gtttggtcgt atggaccagt
    11341 atctctaccc gtactatcgc cgcgacgttg aactcaacca gacgctggat cgcgaacacg
    11401 ccatcgagat gctgcatagc tgctggctga aactgctgga agtgaacaag atccgctccg
    11461 gctcacactc aaaagcctct gcgggaagtc cgctgtatca gaacgttacc attggcggac
    11521 aaaatctggt tgatggtcaa ccaatggatg cagtgaatcc cctctcttac gcgatcctcg
    11581 aatcctgcgg tcgcctgcgt tccactcagc ctaacctcag cgtgcgttac catgcaggaa
    11641 tgagtaatga tttcctcgac gcctgcgtac aggtgatccg ttgcggcttc gggatgccgg
    11701 cgttcaacaa cgacgaaatc gtgatcccgg aatttattaa actcggtatt gaaccgcagg
    11761 acgcttacga ctacgcagcg attggttgta tcgaaaccgc cgtcggtggc aaatggggct
    11821 atcgctgtac cggcatgagc tttatcaact tcgcccgcgt gatgctggcg gcgctggaag
    11881 gcgggcgtga tgccaccagc ggcaaagtgt tcctgccaca agaaaaagcg ttgtcggcag
    11941 gcaactttaa caatttcgat gaagtaatgg acgcgtggga tacgcaaatc cgttattaca
    12001 cgcgtaaatc aatcgaaatc gaatatgtcg tcgacaccat gctggaagag aacgtacacg
    12061 atattctctg ctcggcgctg gtggatgact gtattgagcg agcgaaaagt atcaagcaag
    12121 gcggcgcgaa atatgactgg gtttctggcc tgcaggttgg cattgccaac ctcggcaaca
    12181 gcctggcggc agtgaagaaa ctggtatttg aacagggcgc gattggtcag caacagctgg
    12241 ctgccgcact ggcggatgac ttcgatggcc tgactcatga gcaactgcgt cagcggctga
    12301 ttaacggtgc gccgaagtac ggcaacgacg atgataccgt cgatacgctg ctggctcgcg
    12361 cttatcagac ctatatcgac gaactgaaac agtaccataa cccgcgctac ggtcgtggtc
    12421 cggttggcgg caactattac gcgggtacct cgtctatctc cgctaacgta ccgtttggcg
    12481 cgcaaactat ggcaacaccg gacgggcgta aagcccacac cccgctggca gaaggcgcta
    12541 gtccggcctc tggtactgac catctcggcc ctactgcggt cattggctcg gtgggtaaac
    12601 tgcctacggc agcgattctc ggcggcgtgt tgctcaacca gaaactgaat ccggcaacgc
    12661 tggagaacga atctgacaag cagaaactga tgatcctgct gcgcaccttc tttgaagtgc
    12721 ataaaggctg gcatattcag tacaacatcg tttcccgcga aacgctgctg gaggcgaaaa
    12781 aacatccgga tcagtaccgc gatctggtag tgcgtgtcgc gggctattcc gccttcttca
    12841 ccgcgctctc tccagacgca caggacgata tcatcgcccg tactgaacat atgctgtaat
    12901 ctcctcggcc cggcggcgtc gtcgccgggc taaatcattc acatcatcaa tttcatcctt
    12961 actttcattc gaaatataat ttgtgctctt cgtcacattg ttattacact gttacggttt
    13021 cattttcgag ccaggagcac acctatgagc gtaaaagtta tcgtcacaga catggacggt
    13081 acttttctta acgacgccaa aacgtacaac caaccacatt ttatggcgca atatcaggaa
    13141 ctgaaaaagc gcggcattaa gttcgttgtc gccagcggta atcagtatta ccagcttatt
    13201 tcgttctttc ctgagctaaa ggatgagatc tcttttgtcg cggaaaacgg cgcactggtt
    13261 tacgaacatg gcaagcaact gttccacggc gaactgaccc gacatgaatc gcggattgtt
    13321 attggcgagt tgctaaaaga taagcaactc aattttgtcg cctgcggtct gcaaagtgca
    13381 tatgtcagcg aaaatgcccc cgaagcattt gtcgcactga tggcaaaaca ctaccatcgc
    13441 ctgaaagctg taaaagatta tcaggagatt gacgacgtac tgttcaagtt ttcgctcaac
    13501 ctgccagatg aacaaatccc gttagtgatc gacaaactgc acatagcgct cgatggcatt
    13561 atgaaacccg tcaccagtgg ttttggcttt atcgacctga ttattcccgg tctacataaa
    13621 gcaaacggta tttcgcggtt actgaaacgc tgggatctgt caccgcaaaa tgtggtagcg
    13681 attggcgaca gcggtaacga tgcggagatg ctgaaaatgg cgcgttattc ctttgcgatg
    13741 ggcaatgctg cggaaaacat taaacaaatc gcccgttacg ctaccgatga taataatcat
    13801 gaaggcgcgc tgaatgtgat tcaggcggtg ctggataaca catccccttt taacagctga
    13861 cctctctacc ggagccgtgc gctccggttt tcccttcttt ttacatcctg tacatcacat
    13921 caaatcatta aacttgcatt aacttattta taacttatat tgtcacatgc aaatcagaat
    13981 ggtttcgaat gaaagttaat gagaggtggt tatggcttct acttttacca gcgacacatt
    14041 gcctgccgat cacaaagcag ctatccgtca gatgaagcac gcgctgcggg cgcagcttgg
    14101 cgacgtccag cagatcttta atcagctaag cgatgacatt gccacgcgag tggctgaaat
    14161 caacgcactc aaagcacagg gcgatgccgt ctggccggtg ctgtcttatg ccgatatcaa
    14221 agcaggtcat gttactgcag agcagcgcga acagattaaa cgtcgcggtt gtgcggtgat
    14281 aaaaggccat ttcccccgcg aacaagcgct aggctgggat cagtcgatgc tggactatct
    14341 ggaccgcaac cgctttgacg aggtctacaa aggccccggc gataatttct tcgggacgct
    14401 cagcgcttca cgtcccgaga tttaccccat ctactggtcg caggcgcaaa tgcaggcccg
    14461 ccagagtgaa gaaatggcga atgcgcagtc gtttctcaat cgtctgtgga catttgaaag
    14521 tgatggaaag caatggttta acccggatgt gagcgtcatc taccctgacc gtatccgccg
    14581 ccgtccgccc ggaacgacct ccaaaggtct tggagcgcat accgactccg gggcgctgga
    14641 acgctggctg cttccagcgt atcagcgcgt tttcgccaac gtctttaatg gcaatctggc
    14701 gcaatatgat ccctggcatg cggcacatcg tacggaagtt gaagagtaca cggtggacaa
    14761 caccaccaaa tgttccgtgt ttcggacatt ccagggctgg acagcgctct ctgatatgct
    14821 gcctggtcag gggttgctgc acgttgtgcc cattcctgaa gccatggcgt acgtactgtt
    14881 acgtccgctg cttgatgatg tgccggagga tgaactgtgc ggcgtagcgc ccggaagagt
    14941 gttgccggta tcagagcaat ggcatccact gttaattgag gcgttaacca gcattccaaa
    15001 actcgaggcc ggagactccg tctggtggca ctgcgacgtc atccattccg ttgcccccgt
    15061 tgaaaatcaa cagggttggg gcaacgtgat gtacattcct gcggcaccga tgtgcgagaa
    15121 aaatcttgcc tacgcgcaca aggtgaaggc cgcactggaa aaaggcgcat cgccgggcga
    15181 cttcccgcgc gaggactatg aaacaaactg ggaaggacgc tttacgcttg ccgacctcaa
    15241 cattcacggt aagcgagcgt tgggcatgga tgtttgagag ttaatggaag tgaattgatg
    15301 tgggattaag aaaatcgatt cgacgttact cagattgctg acaagtgcgc tttcttaatg
    15361 cctgatgcgc tacgcttatc tggcctacaa agtcctgcag attcaatata ttacaggtac
//
LOCUS       DAZFXT010000110        15155 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.110, whole genome shotgun sequence.
ACCESSION   DAZFXT010000110 DAZFXT010000000
VERSION     DAZFXT010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15155)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15155)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15155
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.110"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            168..1499
                     /locus_tag="ACHKW0_003688"
                     /pseudo
     CDS             168..1499
                     /locus_tag="ACHKW0_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            1512..2470
                     /locus_tag="ACHKW0_003689"
                     /pseudo
     CDS             1512..2470
                     /locus_tag="ACHKW0_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
     gene            complement(2509..3408)
                     /gene="nhaR"
                     /locus_tag="ACHKW0_003690"
     CDS             complement(2509..3408)
                     /gene="nhaR"
                     /locus_tag="ACHKW0_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HFO7476451.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(3474..4640)
                     /gene="nhaA"
                     /locus_tag="ACHKW0_003691"
     CDS             complement(3474..4640)
                     /gene="nhaA"
                     /locus_tag="ACHKW0_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HFO7476452.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            5226..5378
                     /gene="mokC"
                     /locus_tag="ACHKW0_003692"
     CDS             5226..5378
                     /gene="mokC"
                     /locus_tag="ACHKW0_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HFO7476453.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(5482..6612)
                     /gene="dnaJ"
                     /locus_tag="ACHKW0_003693"
     CDS             complement(5482..6612)
                     /gene="dnaJ"
                     /locus_tag="ACHKW0_003693"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HFO7476454.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(6701..8617)
                     /gene="dnaK"
                     /locus_tag="ACHKW0_003694"
     CDS             complement(6701..8617)
                     /gene="dnaK"
                     /locus_tag="ACHKW0_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HFO7476455.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            8994..9398
                     /gene="yaaI"
                     /locus_tag="ACHKW0_003695"
     CDS             8994..9398
                     /gene="yaaI"
                     /locus_tag="ACHKW0_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HFO7476456.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            9424..10137
                     /gene="msyB"
                     /locus_tag="ACHKW0_003696"
     CDS             9424..10137
                     /gene="msyB"
                     /locus_tag="ACHKW0_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HFO7476457.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            10286..10852
                     /gene="satP"
                     /locus_tag="ACHKW0_003697"
     CDS             10286..10852
                     /gene="satP"
                     /locus_tag="ACHKW0_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HFO7476458.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(10887..11474)
                     /gene="mog"
                     /locus_tag="ACHKW0_003698"
     CDS             complement(10887..11474)
                     /gene="mog"
                     /locus_tag="ACHKW0_003698"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HFO7476459.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(11589..12542)
                     /gene="tal"
                     /locus_tag="ACHKW0_003699"
     CDS             complement(11589..12542)
                     /gene="tal"
                     /locus_tag="ACHKW0_003699"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HFO7476460.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            12821..14251
                     /gene="yaaJ"
                     /locus_tag="ACHKW0_003700"
     CDS             12821..14251
                     /gene="yaaJ"
                     /locus_tag="ACHKW0_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/glycine:cation symporter family protein"
                     /protein_id="HFO7476461.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SLVICVMNIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNGTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            14321..15097
                     /gene="yaaA"
                     /locus_tag="ACHKW0_003701"
     CDS             14321..15097
                     /gene="yaaA"
                     /locus_tag="ACHKW0_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HFO7476462.1"
                     /translation="MLILISPAKTLDYQSPLTTMRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
BASE COUNT         3852 a         3757 c         3570 g         3976 t
ORIGIN      
        1 tctgaagcat actctgaaac atcctactct ctcactctaa taatggcacc ttgctcttct
       61 tataaagaag ggaataatta agaatattaa accttcatca acttcacatc aagaaattaa
      121 aaattttaaa cattaatcta aataataaca acctgatcac ataatcgggt acgattttat
      181 ccgataacta taatgctata actctgggaa atggttggaa tacaccgctg ggtgccatct
      241 ctttttacgc tgcccgatcg agtagtaagt taaataatga taccagacac gaaggtacga
      301 gctatcaggt cgcctataat aaatatctag tacaaacagc cactcgattt agcgtggccg
      361 cctggcgtta tgcttcgcag gattacagga cattcagcga ccatctttac gaaaatgata
      421 aacactatca tcagagtgat tatgatgatt tttatgatat tggcagaaaa aatagccttt
      481 ctgctaatat tatgcaacct ttatccaata atctgggaaa tgtatcatta agtgcgcttt
      541 ggcggaatta ctgggggcga agtggaaatg ctaaagatta ccaattcagt tactccaata
      601 actggcaaca cattagttat actttctctg ccagccaatc ttatgatgaa aataataaag
      661 aagaggagcg ttttaatctg tttatctcca ttcctttcta ctggggggat gatattgcca
      721 aaacacgtca ccaaattaac ttatcgaatt cgacctcatt ttccaaagat ggctattccc
      781 ccaacaatac tggaattact ggcatagccg gtgaacatga tcagttaaat tatggtatat
      841 atgttaatca gcaacaacaa aataatgata cctcgcttgg tacgaattta agctggagaa
      901 ctcccatcgc cataatagat ggcagctata gtcattctaa aaacgcctgg caaagtggtg
      961 gaagtattag tagtggatta gttgtctggc ccggtggtat taatatcact aaccagttat
     1021 ccgatacatt tgcaattctg gatgcgtctg ggttagaagg tgcacatatt aatggacaaa
     1081 aatacaatcg aacaaacagc aaaggccagg ttgtttacga cctgataata cctcatcgtg
     1141 aaaaccatct ggtacttgat atagcaaaca gtgaaagtga aacagaattg cagggcaatc
     1201 gtcaaattat tgcaccttac cgtggagcag tttcttatgt gcagtttaca actgaccaac
     1261 gtaagccctg gtatatacag gcactgcgtc ccgatggttc gccattaacc tttggctatg
     1321 acgtactgga tctccaggaa aataatattg gagtcgttgg ccagggtagt cgccttttta
     1381 ttcgcgtaga tgaaattccc actggaataa aagttgctct caatgatgaa cagaacttat
     1441 tctgtactat tacttttcaa cacgttatcg atgaaaacaa aacatatata tgccagtaaa
     1501 aggagtttac tatgaagtgg cttttattaa tcaccttgag tctatattct tttattgtgc
     1561 aaagtgctcc ctgtgcattg accaatgtcg gtgaacaacg tggaacatat atactcaagc
     1621 cactgagtat gaaaggtaat ctaaccgccg aaaaattgaa aacaccggaa atatcattga
     1681 gtgtacagac ttgcaagaga caaccaacgc cattaaagtt cattttttaa cacagaacgc
     1741 tttaaggttt aaagtcggaa gtcgtcatta tattattaat ttcccctttg aatctgacgg
     1801 taaaaaaata gagcttccag ggaagtcatc ttataattta gaagaaatgc tatctgcgat
     1861 aaatttcacc attccatatt ctatcgcatc tgtatcagat agttctaaca ccgcagacat
     1921 ttttcctgga actccttttc ctttgttgtt cgaaattgaa gtgagttcat gtacagggaa
     1981 taatactaat tgtaatagcg tccgctttaa ttataattta aatactattt tacaggtaaa
     2041 tttaatgacc tgtggttttg aagatcaatc catcgacact ggacgatttg tactgtctcg
     2101 tatcaataat gaaaattatc agtttcatca tataagattt gactgcatcc agggcgagca
     2161 aggtgaatta gtttttgaac ccagtgatgt tgaatattac tttgaaccag tgacgataca
     2221 ggagtcagga accagcatat taaaaaatga gttagaaaac agtgaggacg gtgcgggtca
     2281 aataggcttt caactctcca atgatggtac gaatgaaatc cagtatggta aaagcaatta
     2341 ctactctttt cagcatcccc atgaaggaag taatcagatt cctctattta ttcgcccgcg
     2401 tacatatgga aataatgtct caagcgggca aataatgagc cgagtcaaaa tcgtggttat
     2461 gtacaactaa tctatctccc ccaccttaac tttgggggga ggtgccgatt aacgcgccgc
     2521 tggactaaaa agcgcagaat aatccgtatt gcagattcgc tgtaccgccg ggtgctgaat
     2581 catccgctca gcaaaaatag catggtactc ttccatcaca ttctcgacgc gaccaatttc
     2641 tacgacagtt ttatcggcat aaaagtcata tgcataaagc gttggggcaa cgaagattgc
     2701 attgtgcatc gcaccaaaag ctttcatcaa cgcggcatca tcaaactcgc cgaggatttc
     2761 tacgtttaat ccctgggagt taaaccagtt aagcaatttg cgccctaaca ttgaacgtcg
     2821 cccaggaatc aaaagtcgcc gttcttccag acaagccggg aacggttttt ctggtggtgg
     2881 atttgtacac cagaaactca cgccacattc gccaattctc acggagaaca ggccttcctg
     2941 ctgcgtagag tctatcggac agtcagaaat gatcatatcc agtttatgct gacttaattg
     3001 ctccagcagc atttcgtggg tggattcgaa gcagcgaaga tgaatgggct cgccttctac
     3061 cactgcggcg ttaagtacgc tactgaccag gcgtttggaa agtgcatcag ccacaccaac
     3121 gtcaaacaat aaattggatt ctttgcgata gttcacaata tccagcattt cctggcttaa
     3181 ggtgaacatt ttatcggcat agcgatagac cagttctccc agctcgctgg gttcgagacc
     3241 acgtcccttg cgtttaaata atttgccttg caggcgctct tccagcgctc gaatctgtcc
     3301 ggtaatggtt tgtggagtta aataaagcgc ctccgctgcg ccaaccacgg aaccttcttt
     3361 atagacatgc cagaagtaat acaagtggtt gtaattgata tgagacatgc tcatttctct
     3421 ccctgataac aatgaaaagg gagccgttta tggctccccg gtaaaccgtc ctgtcaaact
     3481 gatggacgca aacgaacgcg taaccagctg tatccaatta ccgccgaaga gatagaaccg
     3541 accaggatac cgagtttcgc ccagttaatc agttctggat ctacgctacc aaaggccagg
     3601 ctggcaataa agatagacat agtaaaaccg ataccgcaca ggatccccac cgccataatt
     3661 tgctgataag tcgttccctc aggcagatgc gccagtttca aacgcagcgc caaccagcag
     3721 aacagactaa tccccagcgg tttgccaatc agcaagccag cgatgatccc caatggcaga
     3781 atggaggtca agccatccag cgtgacacct tgcagtgaaa cgccagcatt agcaaatgca
     3841 aacagcggca aaatcagata cgccacccac gggtgtaaca catgctccag tcgcttcgct
     3901 ggagaacgcc catgcttctc tttcaaagga ataaagaagc cgacaattac ccccgccagg
     3961 gttgcgtgaa cccccgattt caacaccgca gtccacaaca ctacgccaac cagaatatag
     4021 acgcccgtgc ggcgtacacc acacagattc aataccgcga gtaccgcaat tgctacagcc
     4081 gcgacaccaa gagaggccat cgataagtca ttagtgtaga acaatgcgat gataatgatg
     4141 gccccaagat cgtcgataat agccagagcc atcaaaaaga tcttcagcgc taacggaaca
     4201 cgacttccca acagcgccag cacaccaagt gcaaaggcaa tgtcagtagc cgccgggatc
     4261 gcccaccctt cgcgggtaat cggatcggca tagttaaagg ccagatagag taatgccgga
     4321 acaatcatcc caccaatagc ggcgataact ggaaatgcgg cctggcgtaa gctggctagc
     4381 gatccttgca tcagttcacg tttaacttcc agaccgacta acaggaaaaa taccgccatc
     4441 agcgcgtcat ttatccataa cagcatgttt ttgttgattt cgagtgaacc aacccggagc
     4501 tgaaccggcg tctccagaaa gtcgtgatac catccactgg ttgcgccgct attggccata
     4561 atcatcgcca ggatagcggc aatgataaga ataatgcctc ccgaggcatc actgctaaag
     4621 aatcgatgca gatgtttcac tttttatttc tctttcaggt gaatagatcg tttttactat
     4681 tttaccccgc acaaatcatc gttaaaatca gattatagtg taggaataac tcgtttttta
     4741 cgatccattg aagaatttgc gccatcagcg gtgagatgag tttttgacgg ctgtaagcgc
     4801 tcacgagtga ccctaagccc agataatcat agcttaaggt gaggatttct gagagattaa
     4861 ttaggaaaag taataattaa tttgttgtca gtcggcgatg tttgttaggc caatatttca
     4921 aaaataatcc agcttgtcgt caataattgc gatgaagaga taaattaaag ttaaataatt
     4981 aaatcattaa aaaattacgc cataaaaatg cgaggcgtct tcaggattag aatcgataaa
     5041 acagaatggg ttaaagagag cgatataata gcggcgggtg cttgaggctg tctgtctcag
     5101 gcattagctg aacggcagat agagaaaagc cccgagtgat attttaccat caacccgagg
     5161 ccccctatat gctgaacaca tgtagagtgc ctcttactga ccgtaaggtc aaggagaaga
     5221 gagcaatgaa gcagcataag gcgatgattg tcgccctgat cgtcatctgt atcaccgccg
     5281 tagtggcggc gctggtaacg agaaaagacc tctgtgaggt tcacatccga actggccaga
     5341 cggaggttgc tgttttcacg gcttacgaat ccgagtaaga gcaacggcgg ggagtgatcc
     5401 ccgccacttt acaggtgctc gcatatcttc aacgcaccct atttttaccc aggcctgccc
     5461 acgggcaggc ttttggggag gttagcgggt caggtcgtca aaaaacttct tcacaccatc
     5521 aaagaagctc tttgagcgcg ggctgttgtg ctcaccggtt gggccaccga agctttcttg
     5581 cagctcttgc agcagctgtt tctgcttctc gttcaggcct accggtgttt cgacgacaac
     5641 gcggcacagc aaatcaccct gtgcgccacc gcggacagac ttgacgcctt taccgcgcat
     5701 acggaaaagc ttaccggtct gggtttcgcc aggcactttc agtttgacgc gaccatcaag
     5761 ggtcggtact tcgatttcgc cacccagcgc cgccatagcg aagttgatcg ggacttcgca
     5821 atagaggttg ttgccttcac gctcgaaaat cgggtgctgt ttaacctgaa cctgaacgta
     5881 cagatcgcct gccggtgcgc catgctcgcc cgcttcacct tcgcccgcaa gacggatgcg
     5941 gtctccagtg tccacccctg ccgggatttt aacggacagc gttttgctgc gctcaacacg
     6001 accatgacca tgacatttgt tgcacggatc tttgatcagc gtaccgcggc cctgacagtg
     6061 tggacaggtc tgctgtacag cgaagaatcc ctgacgcatc tgcacctgac cagaaccatg
     6121 acaggtcgga caggtctgcg gctgtgtacc tggttttgca ccgctaccgt ggcaaacgtc
     6181 acactcttcc agagtcggaa tgcggatctc tttggtcacg ccacgtacag cttcttcgag
     6241 ggtgagctcc atgttatagc gtaaatcagc accgcgcgcc gcacgttgac gaccacgtcc
     6301 gccgccaaaa atatcgccga aaacgtcacc aaaaatatcg ctgaagtctg cgccgccgcc
     6361 aaaaccgccg ccgcccatac caccttgctc aaacgcagca tgaccatact gatcgtatgc
     6421 cgcacgtttt tgcgagtcgg tcagaacttc ataagcttcc ttgatctctt taaatttcgc
     6481 ctcggcctct ttgtcaccct ggttacggtc cgggtggtat ttcatggcca ggcgtttgta
     6541 ggcctttttg atttcacgct cttccgctgt tttggaaacg cctaaaatct cgtaataatc
     6601 ttgcttagcc atctttttta aattgcccct agatgaatgc acgggcggag aggaaattcc
     6661 ccttcgcccg tgtcagtata attacccgtt tatagggcga ttattttttg tctttgactt
     6721 cttcaaattc agcgtcgaca acatcgtcat ctttcgcgtt gtttgcagaa gcatcagcac
     6781 cggcagtctg ctgctgggca tgttgctgct gggcgatttc catcagtttc tgggaaacct
     6841 gtgccagctc ctgcattttc gcttcgatag cggctttgtc ttcacctttc agagcagttt
     6901 ccagtgcagt cagtgcagac tcgatagcag ttttgtcgtc agccggcagt ttgtcgcctg
     6961 cttcttcaac ctgcttacgg gtgctgtgca gcagatggtc gccctggttg cgagtctgta
     7021 ccagctcttc aaacttacgg tcagcttcgg cgttagcttc tgcgtcgcgt accattttct
     7081 ggatttcatc ttcgttcaga ccagaagaag ccttgatggt gatcttctgc tctttaccgc
     7141 tgtttttatc tttcgcggaa acgtgcagga taccgtcagc atcgatatcg aaggtaactt
     7201 cgatctgcgg catgccgcgc ggtgccgggt tgataccatc caggttgaac tgacccagag
     7261 atttgttatc agccgcacgt ttacgttcac cctgcagcac atggatggtt accgcagact
     7321 ggttgtcttc agcggtagag aacacctggc tgtgcttggt cgggatagtg gtgtttttcg
     7381 cgatcagcgt cgtcatcaca ccgcccatgg tttcgatacc cagagacagc ggggtaacgt
     7441 ccagcagcag tacgtctttc acgtcaccag tcagaacacc accctgaaca gcagcaccga
     7501 ttgctacagc ttcgtccggg ttaacgtctt tacgcggctc tttaccaaag aactcagcaa
     7561 ctttcttctg aaccattggc atacgagtct gaccaccaac gaggataacg tcgtcgatat
     7621 cagatacgga caggccagcg tcctgcagtg caactttcag cggctcaatg gaacggttta
     7681 ccagatcttc aaccaggctt tccagtttcg cacgagtcac tttgatgttc atgtgtttcg
     7741 gaccggtcgc atctgcagtg atgtacggca ggttaacgtc ggtctgctga gcggaagaca
     7801 gttcgatttt cgctttttct gccgcttctt tcaggcgctg cattgccagc ggatcgttgc
     7861 gcaggtcaat gccctgatct ttcttgaatt cttcaaccag gtagttgatc agacggctgt
     7921 cgaagtcttc accacccagg tgggtatcac cgttggtagc cagaacttcg aaggtttttt
     7981 cgccgtcaac ttcgtcgatt tcgataatag aaatatcgaa agtaccacca cccaggtcat
     8041 aaaccgcgat agtacggttg ccagtacctt tgtccagacc gtaagccagc gcagctgcgg
     8101 tcggttcgtt gatgatacgt tttacttcca gaccagcgat acggcctgcg tctttggttg
     8161 cctgacgctg agcatcgtta aagtatgccg gtacggtgat aacagcttca gttaccggtt
     8221 cacccaagta atcttcagcg gttttcttca tttttttcag cacttcagca gagatctgcg
     8281 gcggtgccat tttctggcct ttaacttcga cccatgcgtc gccgttatca gcagcaataa
     8341 ttttgaacgg catgatggaa acatcacgct gtacttcttc gtcctggaag cggcggccaa
     8401 tcaggcgttt aatcgcaaac agggtgtttt gcgggttcgt cactgcctga cgtttagccg
     8461 gctgaccaac cagagtttca ccatcctggg tataggcaat gatagaaggc gtggtgcgat
     8521 cgccttcggc gttctccagc acgcgaggag tggtgccatc cataatcgct acacaagagt
     8581 tggtagtacc caggtcgata ccaattattt tacccatcta aacgtctcca ctatatattc
     8641 ggtcatcatg tggttgtgag tctgtaatag gggcgaaacg tctggtttca actgcccaat
     8701 ttcatttttt tgcagactca ctcactgcgg ttgactacta aatggggtcg taacccacgt
     8761 catcaagggg gagatgcaaa aattttttgt gcttttgcgc agaaatttcg cgatatagca
     8821 ggacaagacg tgaaactgac agcaatttta ttcatataaa gaatgaatcg cagccaagac
     8881 attcatcaaa aaattgtaat aaaaagaaaa cattacgcac ctgaatcttc tctttatcag
     8941 cagtaaacta gtgggtatcc atccccctac ctctccccac taagagaatc cttatgaaat
     9001 ccgtttttac gatttccgcc agcctggcga ttagtctgat gctgtgctgc acggcgcagg
     9061 caaacgacca taaaatcctc ggcgtcattg caatgccgcg taacgaaacc aacgatctgg
     9121 cgttgaaact tcctgtttgc cgaattgtta aacgcataca actctctgcc gaccatggcg
     9181 atttacagtt aagcggtgca tcggtttatt tcaaagccgc ccgtagcgcc agtcagagcc
     9241 tgaatattcc ttcagaaata aaagaagggc aaaccactga ctggatcaac attaacagcg
     9301 ataacgacaa taaacgctgc gtctcaaaaa tcaccttttc cggtcatacg gtgaactcat
     9361 cggatatggc cacgctgaaa attatcggcg acgactaacg ccagaatata gaagccacaa
     9421 aaaatgaatg ttaattacct gaatgattca gatctggatt ttctccagca ttgcagtgag
     9481 gaacagttgg caaatttcgc ccgattgctc acccataatg aaaaaggcaa aactcgcctc
     9541 tccagcgtac tgatgcgcaa cgaactgttt aaatcgatgg aagggcatcc cgagcaacat
     9601 cgccgcaact ggcagctgat tgctggagaa ttacagcatt ttggtggcga tagtatcgcc
     9661 aacaaactgc gcggacacgg taaattgtat cgggccattt tgctcgatgt ttcaaaacga
     9721 ttgaagctga aagccgacaa agagatgtct acgtttgaga ttgagcaaca gttactggaa
     9781 caatttctgc gtaacacctg gaagaatatg gacgaggaac ataagcagga gtttctgcac
     9841 gcgatcgatg ccagggtgaa tgagctggaa gagctgctgc cgctgctgat gaaagacaaa
     9901 ttattggcaa aaggcgtgtc gcatttgctt tccagccaac tgacccgcat tttacgcacc
     9961 cacgcagcaa tgagcgtact tgggcatggt ttgctgcgcg gcgcggggct gggaggccct
    10021 gtaggtgcgg cactaaatgg ggttaaagcg gtcagcggca gcgcctatcg cgtgacgatt
    10081 ccagccgtac tgcaaatcgc ctgcctgcgc cggatggtta gcgccactca ggtctgatac
    10141 cggaaaccgg ggaaaatttt cattttcccc tcaaaagatc gtagacactg ccccactggc
    10201 tgattattat gccgcgccct gaaaacacta cagttattca gggaaattat ttcaccattc
    10261 attcgatgat gatttttgag gaattatggg caacactaag ttggctaatc cggcaccgct
    10321 gggcctgatg ggcttcggca tgaccaccat tctgcttaac ctgcacaacg tgggttattt
    10381 cgctctggac ggtattattc ttgccatggg cattttctac ggcggcatcg cgcaaatttt
    10441 tgctggtctg ctggagtaca aaaaaggcaa cactttcggt ttaaccgcat tcacctctta
    10501 cggttctttc tggctgacgc tggttgcgat tctgctgatg ccgaaactgg gtctgaccga
    10561 tgcgccaaat gcacagttcc ttggtgtcta cctgggtctg tggggcgtat ttacgctgtt
    10621 tatgttcttc ggcacgctga aaggcgcacg cgttctgcaa ttcgttttct ttagcctgac
    10681 cgtgctgttt gccctgctgg cgatcggtaa cattgccggt aacgccgcaa tcatccactt
    10741 tgccggctgg attgggctga tctgcggtgc cagcgcaatc tatctggcga tgggtgaagt
    10801 actgaacgag cagtttggtc gcaccgttct gccgattggt gaatcccact aattgttttg
    10861 agatcccccc gctcgggggg atttttttat tcgctaacgt cgcgtcttgc actcttcggt
    10921 ctgaatgctg caaccacttc cggtgccgtt tcaacgtatg gcccttccag caactgaatg
    10981 cagtacggta cgctggcaaa aataccgtgt accacaacgt tacccgcagc gtccttcaca
    11041 ccttccagcg tctctttaat agacttcggc tgaccgggta agttaaggat cagcgcctgt
    11101 ttgcgaatca cgcccacctg acgcgaaagg atcgcagttg gtacaaaatg caggctgatc
    11161 tggcgcatct gttcaccaaa gccaggcatc tcgcggtccg ctactgccag cgtcgcatcg
    11221 ggcgttacgt cacgacgcgc cgggccagtt ccgcccgtgg tgagcaccag atggcaactc
    11281 atttcatcca ctagctcaca caacgtttgc tcgatgatcg cctgctcatc ggggattaag
    11341 cgggtttcca gttcaaacgg cgtggttagc gccgatgtca gccattcttc cagcgcaggg
    11401 atgcctttat cctgataaac gccgctggat gcgcgatcag agatggaaac taagccaata
    11461 cgtaaagtat tcatcaagat ttttccgttt aaacagtctc gttaaacaga atgatacact
    11521 gcgaagggag tgacagacag gcttcagaag aggtagcgtg accgacttcc cggtcacgct
    11581 aagaatgatt acagcagatc gccgatcatt ttttccagtt tttcctggtc aatagcaaac
    11641 ttacggatac cttccgccag tttatctact gccattggat cctggttgtg ctgccacagg
    11701 aactcggact cagtgatacg cgccggacgc gctttcactt cgccggtgta agacagttta
    11761 cgttcgatag ccccttcgct ctccgccagc tctttcagca gtgccggtgc gatggtcaga
    11821 cggtcgcagc ctgccagttc cagaatttcg ccgatgttac ggaagcttgc gcccataacc
    11881 actgtttcat aaccgtgctc tttgtagtac tggtagattt cagatacaga aaccacgccc
    11941 ggatcttctg ccggagcgta ctctttctta tcagtattcg ctttgtacca gtcaagaata
    12001 cggccaacaa atggcgagat caggaacacg cctgcttccg cacaagcacg agcctgagcg
    12061 aaggagaaca gcagggtcag gttacagttg atgccttctt tttccagctg ttctgcagca
    12121 cggataccct gccaggtaga agccagtttg atcagaatac gatcgttgct aataccagca
    12181 tcgttgtaga gtttgatcag gcgttttgct ttcgcaattg acgcttcggt gtcataggaa
    12241 agacgcgcat caacttcagt tgagatacgg cccggaacca gtttcaggat ttccagacca
    12301 atatttactg ccagtttgtc ggtcgcgtcc acgatctgct gcgcgcgatc gttgctctgc
    12361 tgtttcgccc aagcgacagc atcatcaatc aacttacggt attccggaat ctgcgctgcg
    12421 ttaagaatga gagaagggtt ggttgtggca tcctgcggtt gatacagctt cattgccgcg
    12481 atgtccccag tgtcggccac tacggtggtg tactgacgaa gggaggtcaa tttgtccgtc
    12541 atgatagtat ttctctttaa acagcttgtt agggggatgt aaccggtctg ccctgatgat
    12601 atcacgacgc aacgtcagcg caaccgccga caagacggtt atcgccaggc gagactgttt
    12661 cggatttctg acactgcgtt gatatcgccc gccattttta tacaaaacct catgtatgct
    12721 acgcagaagt tatcaagtac ctcgtagcgt atatacttct taaacaattg gtaacgttta
    12781 cacaggaaag tcatcgcgac cggcaataag agggatatgc atgccagatt ttttctcctt
    12841 tattaacagc gtcctttggg gatcggtaat gatttacctg ctcttcggcg caggttgttg
    12901 gttcactttt cgcaccggat ttgtgcagtt tcgctacatc cgccagtttg gcaaaagtct
    12961 taaaaatagc attcatccac agccaggcgg tttaacctca tttcagtcat tgtgtaccag
    13021 tcttgcggcg cgcgtgggta gcggcaacct ggccggcgtt gcgctggcta ttaccgccgg
    13081 tggacctgga gccgtctttt ggatgtgggt tgccgcgttt atcggcatgg cgacctcgtt
    13141 tgccgaatgt tcccttgcac aactttataa agaacgtgac gtcaatggac agtttcgtgg
    13201 aggaccggca tggtatatgg cgcgcgggct ggggatgcgt tggatgggcg ttctgttcgc
    13261 cgtctttttg ctcatcgcct acggcataat tttcagcgga gttcaggcga acgccgttgc
    13321 ccgagccctg agtttttctt ttgattttcc cccgctggtg acaggcatta ttctcgctgt
    13381 ctttgctctg ctggcaatca ctcgcggtct tcatggcgtc gcccggctca tgcagggttt
    13441 tgtcccgttg atggcgataa tctgggtact gaccagcctg gtgatttgcg taatgaatat
    13501 cgggcaactt ccccacgtca tttggtctat ttttgagagt gcttttggct ggcaggaagc
    13561 ggcaggcggc gcggcgggat ataccttaag ccaggcgatt actaacggtt ttcagcgcag
    13621 tatgttttcc aatgaggcgg gaatgggttc tacaccaaac gcagcagcgg cagctgcgtc
    13681 ctggcctccg catccggcag cgcaagggat tgtccagatg attggcattt ttatcgacac
    13741 cctggtcatc tgtacggcaa gcgccatgct gatattactg gcgggtaacg gcacaaccta
    13801 catgccgctg gaaggtattc agcttatcca gaaggcgatg cgggtgctaa tgggttcctg
    13861 gggtgctgag tttgttaccc tcgtggttat tctgtttgcc ttcagctcca tcgttgccaa
    13921 ctacatttat gccgaaaaca atctcttctt tttacgcctg aacaacccta aagcgatctg
    13981 gtgtttgcgg atctgcacct tcgcaacggt catcggcggc accttgctaa gtcttccgct
    14041 gatgtggcaa ctggcagata tcataatggc ctgcatggct attaccaatt tgaccgccat
    14101 tttactgctc tcgcctgtgg ttcataccat tgccagtgat tatctacgcc agcgtaaact
    14161 cggcgtgcgc ccggtgtttg atccgttgcg ttatccggat atcggtcgcc agctttctcc
    14221 ggacgcgtgg gatgatgttt cgcaggagta atcacaacta tcgatcaact cattctcttt
    14281 ttttgctaaa gtcggcataa atttcctgca aggactggat atgctgattc ttatttcacc
    14341 tgcgaaaacg cttgattacc aaagcccgtt gaccaccatg cgctatacgc tgccggagct
    14401 gttagacaat tcccagcagt tgatccatga agcgcggaaa ctgacgcctc cgcagattag
    14461 cacgctgatg cgcatcagcg acaaactggc gggtatcaac gccgctcgct ttcatgactg
    14521 gcagccagat ttcacgccgg agaatgcccg ccaggcgatt ctggcgttta aaggtgatgt
    14581 ctacaccggc ttgcaggccg aaaccttcag cgaagacgat ttcgattttg cccaacagca
    14641 tttgcgaatg ctttccggct tgtatggcgt actgcgcccg ctcgatttaa tgcagcctta
    14701 tcgtctggaa atggggatcc gtcttgagaa tgcccgaggg aaagatctgt atcaattctg
    14761 gggagatatc atcaccaaca agctgaacga ggcgctcgca gcacaaggcg ataatgtggt
    14821 gattaatctg gcgtcagatg agtattttaa atcggtgaag ccgaagaaat tgaatgccga
    14881 gattatcaag ccggtgttcc tcgatgagaa gaacggcaaa tttaagatca tcagcttcta
    14941 cgctaagaaa gcacgcggtc tgatgagtcg tttcattatt gaaaatcggc tgaccaaacc
    15001 agagcaactg actggtttta atagcgaagg ttacttcttt gatgaagctt cctccagcaa
    15061 tggtgaactg gtgtttaaac gctacgagca gcgttaagtt tgtagccaat gccggatgcg
    15121 gcgtgaacgc cttatccggc ctacaaaagc atgca
//
LOCUS       DAZFXT010000111        15087 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.111, whole genome shotgun sequence.
ACCESSION   DAZFXT010000111 DAZFXT010000000
VERSION     DAZFXT010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15087
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.111"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..1224
                     /gene="citC"
                     /locus_tag="ACHKW0_003702"
     CDS             166..1224
                     /gene="citC"
                     /locus_tag="ACHKW0_003702"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008771 - [citrate (pro-3S)-lyase] ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006101 - citrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HFO7476463.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1239..1535
                     /gene="citD"
                     /locus_tag="ACHKW0_003703"
     CDS             1239..1535
                     /gene="citD"
                     /locus_tag="ACHKW0_003703"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HFO7476464.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1532..2440
                     /gene="citE"
                     /locus_tag="ACHKW0_003704"
     CDS             1532..2440
                     /gene="citE"
                     /locus_tag="ACHKW0_003704"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006084 - acetyl-CoA metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HFO7476465.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2451..3983
                     /gene="citF"
                     /locus_tag="ACHKW0_003705"
     CDS             2451..3983
                     /gene="citF"
                     /locus_tag="ACHKW0_003705"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009346 - ATP-independent citrate lyase
                     complex [Evidence IEA];
                     GO_function: GO:0008814 - citrate CoA-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0008815 - citrate (pro-3S)-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HFO7476466.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3987..4538
                     /gene="citX"
                     /locus_tag="ACHKW0_003706"
     CDS             3987..4538
                     /gene="citX"
                     /locus_tag="ACHKW0_003706"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050519 - holo-citrate lyase synthase
                     activity [Evidence IEA];
                     GO_process: GO:0051191 - prosthetic group biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HFO7476467.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRSCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4513..5391
                     /gene="citG"
                     /locus_tag="ACHKW0_003707"
     CDS             4513..5391
                     /gene="citG"
                     /locus_tag="ACHKW0_003707"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HFO7476468.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5442..6905
                     /gene="citT"
                     /locus_tag="ACHKW0_003708"
     CDS             5442..6905
                     /gene="citT"
                     /locus_tag="ACHKW0_003708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HFO7476469.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7019..7825
                     /gene="rna"
                     /locus_tag="ACHKW0_003709"
     CDS             7019..7825
                     /gene="rna"
                     /locus_tag="ACHKW0_003709"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HFO7476470.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANALALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8055..8465
                     /gene="rnk"
                     /locus_tag="ACHKW0_003710"
     CDS             8055..8465
                     /gene="rnk"
                     /locus_tag="ACHKW0_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HFO7476471.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLRIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            8603..8692
                     /gene="yldA"
                     /locus_tag="ACHKW0_003711"
     CDS             8603..8692
                     /gene="yldA"
                     /locus_tag="ACHKW0_003711"
                     /inference="COORDINATES: protein motif:HMM:NF041476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YldA"
                     /protein_id="HFO7476472.1"
                     /translation="MAEAFYILIGFLIMAAIIVMAVLYLENHS"
     gene            complement(8696..9934)
                     /gene="ybdR"
                     /locus_tag="ACHKW0_003712"
                     /pseudo
     CDS             complement(8696..9934)
                     /gene="ybdR"
                     /locus_tag="ACHKW0_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase (NAD+)
                     activity, zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
     gene            10155..10583
                     /gene="uspG"
                     /locus_tag="ACHKW0_003713"
     CDS             10155..10583
                     /gene="uspG"
                     /locus_tag="ACHKW0_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HFO7476473.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10704..12269)
                     /gene="ahpF"
                     /locus_tag="ACHKW0_003714"
     CDS             complement(10704..12269)
                     /gene="ahpF"
                     /locus_tag="ACHKW0_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008785 - alkyl hydroperoxide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000302 - response to reactive oxygen
                     species [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HFO7476474.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12398..12961)
                     /gene="ahpC"
                     /locus_tag="ACHKW0_003715"
     CDS             complement(12398..12961)
                     /gene="ahpC"
                     /locus_tag="ACHKW0_003715"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HFO7476475.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13333..14079
                     /gene="dsbG"
                     /locus_tag="ACHKW0_003716"
     CDS             13333..14079
                     /gene="dsbG"
                     /locus_tag="ACHKW0_003716"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HFO7476476.1"
                     /translation="MLKKILLLALLPAIAFAEERPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14289..>15087
                     /locus_tag="ACHKW0_003717"
     CDS             14289..>15087
                     /locus_tag="ACHKW0_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7476477.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKL"
BASE COUNT         3666 a         3936 c         3729 g         3756 t
ORIGIN      
        1 atttcacgcc gaaatactcc ttaggatgta tagcgaaagg agaaaaagat atacctcgat
       61 cacccccctt tctcccaagt gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt
      121 cgttgcaaat cgataacaac atgcaccttc aggatactat ttattatgtt cggcaatgat
      181 attttcaccc gcgtaaaacg ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg
      241 catgaaaatg atttgagcgt tgacaccaca gtcgaagtat ttattaccgt aacccgcgat
      301 gaaaagctta tcgcgtgcgg tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt
      361 gaatccgttc gcggtgaagg actggcgctg acattagcca ctgaactgat aaacctcgcc
      421 tatgagcggc acagcacgca tctgtttatt tataccaaaa ccgaatacga ggcgctgttc
      481 cgccagtgcg gtttttccac gctgaccagc gtacccggcg tgatggtgct gatggaaaac
      541 agcgccacgc ggctgaaacg ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac
      601 aagattggct gcattgtgat gaacgccaat cccttcacga atggtcaccg ttatctgatt
      661 caacaagctg cggcacagtg cgactggttg catctgtttt tagtcaaaga agattcttca
      721 cgcttcccct atgaagaccg gttggatctg gtgttaaaag gcaccgccga tattccacgc
      781 ctgactgtgc atcgcggctc cgaatacatc atctcccgcg ctacgttccc ttgctacttc
      841 attaaagaac agagcgtcat taaccattgt tacaccgaaa ttgatctgaa gattttccgt
      901 cagtacctcg ctcccgcgct gggtgtaact caccgctttg tcggtactga acccttttgt
      961 cgcgttaccg cccagtacaa ccaggatatg cgctactggc tggaaacgcc gactatctcc
     1021 gcaccgccca tcgaactggt tgaaattgag cggctgcgtt accaggagat gccgatatcc
     1081 gcttcccggg tacgtcaact gctggcgaaa aacgatctca cggctatcgc gccgctggtc
     1141 cctgcagtca cgctgcatta tttgcagaac ctgcttgagc actcccgcca ggacgcggca
     1201 gctcgtcaaa agacccccgc atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg
     1261 ttgcaggcac ccttgagtct ggggatgtga tgatacgcat cgccccactc gatacgcagg
     1321 atatcgacct gcaaatcaat agcagcgttg agaaacagtt tggcgatgca attcgcacca
     1381 ccattctgga cgttctcgcc cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca
     1441 aaggcgcact ggactgcatt ttacgtgcac gactggaagc cctgctggca cgcgccagcg
     1501 gtatcccggc tctgccatgg gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa
     1561 aactcgcacc cgccgcagca tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa
     1621 ctccttcatc tacccggctg atgccctgat gtttgacctc gaagactccg tggcattacg
     1681 tgaaaaagac accgcccgcc gcatggttta tcacgcgctg caacatccgc tgtatcgcga
     1741 tattgaaacc attgtgcgtg tcaacgcgct ggattccgaa tggggtgtta acgacctgga
     1801 agccgtcgtt cgcggtggtg cggacgttgt gcgtctgccg aaaaccgata ccgctcagga
     1861 tgttctggat attgaaaaag agatcctgcg tatcgaaaaa gcctgtggtc gtgaacccgg
     1921 cagcaccggc ctgctggcgg cgattgaatc tccgctgggc attacccgcg cagtggaaat
     1981 cgctcacgct tccgagcgtt tgatcggtat cgccctcggt gcagaagact atgtgcgcaa
     2041 cctgcgtaca gaacgctccc cggaaggaac tgaactgctg ttcgcacgct gttccatttt
     2101 gcaggccgcg cgctctgcgg gtattcaggc gttcgatacc gtctattccg acgctaacaa
     2161 cgaagccgga ttcctgcaag aagccgccca catcaaacag ctgggctttg acggcaaatc
     2221 gctgatcaac ccgcgtcaga ttgatctgtt gcacaacctc tacgcaccaa cccagaaaga
     2281 agtggatcac gcccgccgcg tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg
     2341 cgtggtttcc ctgaacggca agatggtgga cggtccggtt atcgatcgcg cccgtctggt
     2401 gctctctcgt gcagaacttt ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga
     2461 aaattgaaca atctcaacga caagaacggg tagcggcctg gaatcgtcgc gctgaatgcg
     2521 atcttgccgc tttccagaac tcgccaaagc aaacctacca ggctgaaaaa gcgcgcgatc
     2581 gcaaactgtg cgccaacctg gaagaagcga ttcgtcgctc tggtttacag gacggcatga
     2641 cggtttcctt ccatcacgct ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg
     2701 tcatcgcgaa gatgggcttt aaaaacctga ccctggcgtc cagctccctg agtgattgcc
     2761 atgcgccgtt ggtagaacac attcgccagg gcgtggttac ccgcatttat acctccggcc
     2821 tgcgcggtcc actggcggaa gaaatctccc gcggtctgct ggcggaaccg gtgcagatcc
     2881 actctcacgg cggtcgtgtg catctggtac agagcggcga actgaatatc gacgtggctt
     2941 tcctcggcgt cccgtcctgt gatgaattcg gtaatgccaa cggctacacc ggtaaagcct
     3001 gctgcggctc cctcggctat gcaatggttg atgccgacaa cgcaaaacag gttgtgatgc
     3061 ttaccgaaga actgctgcct tatccgcata atccggcaag cattgagcaa gatcaggttg
     3121 atttaatcgt caaagttgat cgcgttggcg atgctgcaaa aatcggcgct ggtgctaccc
     3181 gtatgaccac taacccgcgc gaactgctta ttgcccgtag cgctgcggat gtgattgtca
     3241 actcaggcta cttcaaagaa ggtttctcca tgcaaaccgg caccggcggc gcatcgctgg
     3301 cggtaacccg tttcctggaa gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc
     3361 tcggtggtat taccgcgacg atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc
     3421 tggatgtaca gagctttgac agccacgctg cgcaatcgct ggcccgtaac cccaatcaca
     3481 tcgaaatcag cgccaaccag tacgctaact ggggttcgaa aggtgcatcg gttgatcgtc
     3541 tcgacgtggt ggtactgagc gcgctggaaa ttgacaccca gttcaacgtt aacgtgctga
     3601 ccggctctga cggcgtactg cgtggtgctt ccggtggtca ctgcgatacc gcgattgcct
     3661 ctgcgctttc catcatcgtc gcgccgctgg tacgcggtcg tattccgact ctggtggata
     3721 acgtgctgac ctgcatcacc ccgggctcca gtgtcgatat tctggtcaca gaccacggta
     3781 tcgcagttaa cccggcacgt ccggagctgg cagaacgtct gcaggaagcg ggcattaaag
     3841 tggtttccat tgagtggctg cgtgaacgtg cgcgtctgct gaccggtgaa ccacagccga
     3901 ttgaattcac cgaccgcgtc gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg
     3961 ttgtacatca ggtgaaggaa taagccatgc acctgcttcc tgaactcgcc agccaccatg
     4021 cggtgtcaat tcccgagctg ctcgtcagcc gggatgaaag gcaagcacgg caacacgtct
     4081 ggctcaagcg ccatcctgtt ccactggtct cctttaccgt ggttgcgcct gggccgatta
     4141 aagacagtga ggtcacacgc cgaattttta atcatggcgt gacagccttg cgtgccttag
     4201 ccgcaaaaca gggctggcaa attcaggagc aggctgcact ggtttccgcc agcgggccgg
     4261 agggtatgtt gagcattgcc gccccggctc gcgacctcaa gctcgccacc attgagcttg
     4321 aacatagtca tcctctcggg cggttatggg atatcgatgt cctgacgccc gaaggcgaaa
     4381 ttctctcccg ccgcgactat tcactgccgc ctcgcagctg cctgttgtgc gagcaaagcg
     4441 cagccgtctg cgcgcgtgga aaaacccatc aactgaccga tttactcaac cgcatggagg
     4501 cactgctgaa cgatgtcgat gcctgcaacg tcaactaaaa ccacaaagct tgcgacgtca
     4561 ttaatcgatg agtacgccct gctgggctgg cgcgccatgc tgactgaagt caatctgtca
     4621 ccgaaaccag gcctcgtgga tcggattaac tgcggtgcgc ataaagatat ggcgctggaa
     4681 gatttccacc gcagcgcgct ggcgattcag ggctggctac cccgtttcat tgaatttggt
     4741 gcctgtagtg cggaaatggc accagaagcg gtactccacg gattacgccc aattggtatg
     4801 gcttgcgaag gtgatatgtt ccgcgccact gcgggcgtaa acacgcataa aggcagcatt
     4861 ttttctttag ggctgctatg tgcggcaatt ggccgtttgc ttcagctcaa ccagccggta
     4921 acgccaacaa ccgtttgttc tacggcggca agtttctgcc gtggtctgac cgatcgcgaa
     4981 ctgcgtacca ataattcaca actgacggca ggtcaacggt tgtaccagca gcttggcctt
     5041 accggcgcac gcggtgaagc cgaagcgggt tatccactgg tgatcaacca cgccttgccg
     5101 cattacctca ctctgctgga tcaggggtta gatcctgaac tggcattgct cgataccttg
     5161 ctcctactga tggcgatcaa cggcgatacc aacgttgctt cgcgcggtgg cgaggggggc
     5221 ctgcgctggc tacagcgcga ggcgcaaaca ttattgcaaa aagggggcat tcgaaccccc
     5281 gccgatctcg attatctccg gcagttcgac agggagtgta tcgaacgaaa tctcagtcca
     5341 ggcggcagtg ccgacctgct gatccttacc tggtttttag cacagattta attatttaag
     5401 cacttgataa atttgaaaat attaattttc ggagaacccg tatgtcttta gcaaaagata
     5461 atatatggaa actattggcc ccactggtgg tgatgggtgt catgtttctt atccctgtcc
     5521 ccgacggtat gccaccacag gcatggcatt acttcgctgt gtttgtggca atgattgtcg
     5581 gcatgatcct cgagccaatt ccggcaacag cgatcagttt tattgcggtt actatttgcg
     5641 ttattggcag taattacctg ctctttgatg ccaaagaatt agctgaccca gcgtttaatg
     5701 cgcaaaaaca ggcgctgaaa tggggtctgg ctggtttttc cagcaccacg gtatggctgg
     5761 tatttggcgc atttattttt gcattagggt atgaagtttc cgggttaggt cgtcgcattg
     5821 cccttttcct ggtgaaattc atgggcaaac gcacgctgac gttgggttat gcgattgtca
     5881 ttatcgacat tctgctggca ccgtttacac cgtccaacac cgcgcgtacc gggggtacgg
     5941 tttttccggt cattaaaaac ctgccgccgt tgtttaaatc attccccaac gatccgtccg
     6001 cgcgtcgtat tggcggctat ttgatgtgga tgatggtcat tagtaccagt ctgagttcgt
     6061 ccatgtttgt caccggtgcg gcaccaaacg tgctgggtct ggagttcgtc agcaaaattg
     6121 ccggtatcca gattagctgg ttgcagtggt tcctctgctt cctgccggtt ggggttatct
     6181 tgcttatcat tgcgccgtgg ctttcctacg tgctgtacaa accggaaatc acacacagtg
     6241 aagaagtggc aacctgggcg ggtgatgaac tgaaaaccat gggtgcgctg acacgcagag
     6301 agtggacact gattggcctt gtattgctca gcttaggttt gtgggttttt ggcagtgaag
     6361 tcattaatgc tactgcagtt ggtctgctgg cagtttcgct aatgctggcc ctgcacgttg
     6421 tgccttggaa agatattacc cgctataaca gcgcatggaa cacgctggtc aacctggcaa
     6481 ctctggttgt gatggctaac ggcctgactc gttctggctt tattgactgg ttcgccggta
     6541 ccatgagtac gcacctggaa ggattctcac caaacgcaac ggtgattgta ctggttctgg
     6601 tgttctactt tgcacactac ctgtttgcca gcctgtctgc gcacaccgca accatgctgc
     6661 cggttattct ggccgtcggt aaaggtattc cgggcgtacc aatggaacaa ctgtgtatcc
     6721 tgctggtgct gtctatcggt atcatgggct gtctgacgcc gtatgcaacc ggtcctgggg
     6781 tgattattta cggctgtggc tatgtgaaat caaaagatta ctggcgtctt ggcgcaatct
     6841 tcggggtgat ttacatctct atgttgctgt tggttggctg gccgattctc gccatgtgga
     6901 actaatacgt tcgccaaacg ctgaataaat catcacgccc gccaggtaag cccacctggc
     6961 gggctttttt atgatttaat agatagtatt cagtgtatgt atgagttcca cacccattat
     7021 gaaagcattc tggcgtaacg ccgcgttgct cgcggtttct ctgcttccct tctcttctgc
     7081 caacgcctta gcgttgcagg caaaacagta tggcgatttt gatcgctatg tcctggccct
     7141 ctcctggcaa accggatttt gccagagtca acacgatcga aatcgtaacg aacgagatga
     7201 atgtcgcctg caaaccgaaa cgaccaacaa agctgatttt ctgaccgtac atggtctgtg
     7261 gccaggattg cctaaatcgg ttgctgcccg tggtgttgat gaacgccgct ggatgcgctt
     7321 cggttgcgct actcgcccaa tcccgaatct accagaagcg cacgccagcc ggatgtgttc
     7381 atcgccggaa accggattat cactggaaac ggccgctaaa ctaagtgaag tcatgccagg
     7441 agctggcgga cgttcctgcc tggaacgcta cgaatatgcc aaacacggtg cctgctttgg
     7501 ttttgatccg gacgcatact tcggtacgat ggtacgcctg aatcaagaaa ttaaagagag
     7561 cgaagccgga aaattccttg cggataatta cggtaaaaca gtgagccgcc gtgactttga
     7621 cgccgccttt gccaaaagct ggggaaaaga gaacgtgaaa gcagttaagc taacgtgcca
     7681 gggtaaccct gcgtatttga ctgaaattca gatctcgatc aaagctgacg ccatcaacgc
     7741 tccgctttct gcaaactcat tcttgccaca accacaccca ggcaactgtg gcaaaacctt
     7801 tgtgattgat aaagtgggtt attaatcatc tgatttacaa aacacgctaa ttcgtcttcc
     7861 ataaaccgtg acgattaaga attatacata atgcgctgca ttgaggaatt ccctgaatgt
     7921 gacgcaaatc acttcaaaga gtgagcaaca gtcagtatca tcatgtgagt taaaccctcg
     7981 ccgcctgacg gtgagggttt tcttttgggt atgtttatct gcgacactcg cagtaccgac
     8041 gacatggagt aaaaatgtcc agaccaacta tcatcattaa cgacctggat gccgaacgca
     8101 tcgatattct gctggagcaa cccgcctatg ctggtttgcg aatcgccgac gcgttaaacg
     8161 cagagttgga tcgcgcccaa atgtgttcgc cagaagagat gccacacgac gtggtgacaa
     8221 tgaacagccg ggttaaattc cgcaatctta gcgatggcga agtgcgtgtg cgcacgctgg
     8281 tgtatccggc aaaaatgacc gatagcaata ctcagctttc cgttatggct ccggtaggtg
     8341 ccgcactgct ggggctgcgc gttggcgatt ccattcactg ggaacttccg ggcggcgttg
     8401 caacccacct tgaagtgctg gaactcgaat accagccaga agctgctggc gactacctgc
     8461 tttaatccca tctgcacaag cgataactcg ctttgcccag aggatgcact cgcatcctct
     8521 ttacgcaaaa tcagcaattt ttcctggttc tcactgttca cttttgctgc aaaattattc
     8581 cgtttcgttt gtgggaataa ccatggcaga agcattttac atcctgatag gatttctgat
     8641 tatggcggcg attatcgtca tggctgtcct ttacctcgaa aaccactcct gacgatcata
     8701 ttgttccccc cggcatcgca ttcaccagac ctgaaaccgc cttctgcgcc gcctctgcgc
     8761 tttgtgcacc gggtaccaga atcaccttac ggcactcctc ttcacgtttt tcgaaaatct
     8821 catatccccg ggcggcctct taaaacggca tatagtgggt aacaatttct tctggtttca
     8881 gtaatccttt ctcaattaac ggtaataatt ctcccagcca tgcgtgaacg tgggtctgtc
     8941 ccattttaaa cgtcaaccct ttatcaaagg cgtcgccaaa caggaaaccg tgaataaatc
     9001 cagcgtagac gcccggtacg ctaacaatgc cgccacgcct gaccgccgca atacactggc
     9061 gcaacgcttt accgctgctg ccctccagtt tcaggttagt cagcaccgtt tccgtggtgc
     9121 tgcctttcgc ttcaaaaccg acggcgtcta ttactgcatc cacgccccgg tgacctgccg
     9181 tttgttcaat aattgactgt gccggatcgc tatcttcatc gaaattaatc gggatcgcgc
     9241 cgtagcggtc agcggcgaaa cgcaagcggt agggatgatg atcaacaaca aaaatctgtt
     9301 ccgcaccgag caaccgtgca caggcgattg tcaacaatcc cactggaccg gcaccataga
     9361 ctgcaacgct tgaaccttgt tggatctgcg cattttttgc tgcctgccat gccgttggca
     9421 gaatatcaga aaggaaaagg gctttatcat ctgaaagcaa aggcggtact ttaaacggcc
     9481 ccacattccc tttagggacg cggacatatt ccgcctgtcc accaggaacg ccaccataca
     9541 ggtgactata accaaacaat gccgcgggag ctggtatctg ttttttattg agcgcagcgc
     9601 ctttacccgc attggtattt tcgcaggcgg catattgctg cattcgacag aaaaaacagt
     9661 cgccacaagc aatgacaaac ggaattacca cccggtcgcc tttttgcaaa ttttttacgt
     9721 cctttccggt ttcaacgact tcccccataa attcatgacc aaaaatatcg ccatgtttaa
     9781 cctggggtat tttgcctcga taaagatgga ggtcagagcc acagatcgcc gttgccgtaa
     9841 tacgcagaat aatatcatct gcctgttcaa tgcccggatc gggaacattt tctacctgaa
     9901 cgtgatgtgg gccgtgataa gtcaatgctt tcatgcaacc tccagagaca gttcaaaaaa
     9961 gagtcagtct ttaagggtag ctatcacagc gggaaaacgg gcggtaccgc aggttttggg
    10021 agaattcttg gtgttcagtg agaatatatt aatcacagaa accgttcgta aggaaaaaga
    10081 accagacaaa tatcagggat aaatattata atcatggcga atgatttgtt catgattaac
    10141 agggagaaag ggttatgtat aagacaatca ttatgccagt tgatgtattt gaaatggaat
    10201 tgagcgacaa agctgttcgc cacgctgaat tcctcgccca ggatgacgga gttattcatc
    10261 tacttcacgt actacccggg tcagccagcc tgagcctgca ccgttttgcc gctgatgtgc
    10321 gtcgttttga agagcatctg caacatgaag cagaagaacg tctgcaaacg atggtcagcc
    10381 acttcaccat cgatccttcc cgcattaaac aacatgtccg ttttggtagc gtgcgggatg
    10441 aagtcaatga gttggcagaa gaactggggg ctgatgttgt agttattggt tctcgcaacc
    10501 catcgatttc gacccatctg ttaggttcta acgcctcgag cgtgatccgc catgccaatc
    10561 tgccggtgct ggttgtgcgt taattgccag aataaatatc ccgccctgcc cggagccatc
    10621 ccggcagggc tttttaatgc cataaaaaag ccgccaggtt tgaccccggc ggctaagcaa
    10681 ttgcaggtga atcttacttc ttcttatgca gttttggtgc gaatcaggta gtcaaaagca
    10741 ctcagagagg ctttggcacc ttcgccagtg gcgatgatga tctgcttgta cggaaccgtc
    10801 gtacagtcac ccgctgcgaa tacgcctttg acgttggttt cgcatttcgc atcaatgata
    10861 atctcaccca tgcggttacg ttcgactgcg ccttccagcc agttggtgtt cggcagcaga
    10921 ccaatctgga cgaaaatacc ggccagttcg atgttgtgaa tatcgccgct gacacgatct
    10981 cgatattcca gaccaacgac tttgctgccg tcgcctttca cttccgtggt ttgcgcattc
    11041 agaataatgt cgacgttttt caggctgcgc agtttgtcct gcagcacctg gtcggctttc
    11101 atttctggcg caaattccag cagagttacg tgctcaacga tacccgccag gtcgattgcc
    11161 gcttccacgc cggagttacc gccgccgata accgctacgc gtttgccttt aaacagcggg
    11221 ccgtcgcagt gcgggcagta ggtcacgcct ttggtgcgat actgatcttc gcccggaacg
    11281 ttcatgttgc gccattttgc accggtcgcc acgataatgc tgcgtgcttt cagtaccgcg
    11341 ccagaagctg tttcaatctg gtgtagacca ccttcaaccg ctgccgggat cagtttgctg
    11401 gcgctctggc tgtcgatcac atcaacgtcg tattcatcaa cgtgaacttt cagtgcgcct
    11461 gccagcttct gcccttcagt cttcggtaca gaaatgtagt tttcgatatc aacggtatcg
    11521 aggatctgac caccaaaacg ttcgcccatc agaccggtac ggatgccttt acgtgcggag
    11581 taaattgctg ccgctgcacc cgccgggccg gaaccgacga ttaatacgtc ataagcatca
    11641 cgcttgttca gctcttctgc cgcacgtttt tccgcgccag tatcaatttt ggcaacgatt
    11701 tcagtcaacg tcatgcggcc ctgaccaaac tctttcccgt ttacgaacac tgccggaacg
    11761 cccatcacgt tgcgatcggt gatttcgttc tggaaggtgc cgccgtcaat tgcagtgtgc
    11821 ttgatgcgcg ggttcagtac gctcatcagg ttcagcgcct gcaccacgtc cgggcagttg
    11881 tggcaagaga gcgagtaata ggtttcgaat tcaaaatcac cgtcaatatg gcgaatctgc
    11941 tccagcagag actgcgcttc tttcgacgga tgaccaccgg tccacagcaa cgccagtacc
    12001 agcgaggtga actcgtggcc cagcggggag cctgcaaaac gtggcccctg gttggaacct
    12061 gggttggtga tcaggaaaga cggcttacgc accggcaagc tgttatcttc tttaaaggtg
    12121 actttgtctg acagttctgc gatttcagcc aacagttcct tgatttctgc cgatttagcg
    12181 ctgtcatcca gcgtggcaat taactcaaca ggcttggtca atttctcaag gtaagccttg
    12241 agttgagttt tcatatttgt gtcgagcatg tttatctcct gggctttaaa catcatcatg
    12301 caagcggcct ctgcggctac ctggatgcag cttgcatcat ggtgcgggga agaaaatcgg
    12361 gtgccgcagc acccgattaa ttacgcgatt gcggaactta gattttacca accaggtcca
    12421 gagacggagc cagagttgct tcaccttctt tccatttagc cgggcaaact tcacctgggt
    12481 gagaagctac gtactgtgct gctttgattt tacgcagcag gtcagacgcg tcacggccaa
    12541 tgccttcagc ggtaacttcg attgcctgga tgataccctg cgggtcaaca acgaaggtcg
    12601 cacggtcagc cagaccttca tcttcacgca tgttgtcgaa gttacgggtc agggcgccag
    12661 tcgggtcgcc gatcatcgca tatttgattt tagcgatggt ttcagagctg ctgtgccatg
    12721 ctttgtgggt gaagtgagta tcggtagata ctgcgtatac gtctacgccc agtttctgca
    12781 gttcttcgta gtggtcagca acgtcaccca gttcggtcgg gcatacgaaa gtaaagtcag
    12841 ccgggtagaa gaagaagacg ctccagcggc cttcggtatc tttttcggtg atttcgatga
    12901 attcgccgtt tttgaatgcc tggtttttaa aaggtttaat tttggtgtta atcaaggaca
    12961 tctatacttc ctccgtgttt tcgatgagat gtaaggtaac caatttttgc cgattcggct
    13021 aatgcgtttc cattatcaaa tcgataagtt ttaccttaca accttcgtaa gacaactttg
    13081 tgaactttgc accgccagcg gcgataaaaa gtaaaatggc cccctttcca ggtgaccgtg
    13141 taatctgaac taccctttgg tatcttcagc gcctgcctgc aggctgaggt gttgccctta
    13201 caaatgcaac aacgacatgg attacaacac cctcataaac aaagggcaat cacctgatct
    13261 aagctcttac ctatgacagt gataggttat gccttttact cgacttttgc actgactgaa
    13321 aaggacaaat taatgttaaa aaagatactt ttactggctc tgcttcctgc aatcgccttc
    13381 gcagaggaac gtccttcccc agtaaaagcg attgaaaaac agggcattac aatcatcaaa
    13441 acgttcgatg cccccggagg aatgaaaggt tatctcggaa agtatcagga tatgggcgtc
    13501 accatctacc tgactcccga tggtaagcac gctatctctg gttacatgta caacgagaaa
    13561 ggtgaaaacc tgagtaacac acttatcgaa aaagaaattt acgcaccagc cggacgcgaa
    13621 atgtggcaac ggatggaaca atcccactgg ctcctcgacg gtaaaaaaga tgcgccggtc
    13681 attgtctacg tgttcgccga tccgttctgc ccatattgta aacagttctg gcagcaggcg
    13741 cgcccgtggg tagattctgg caaagtgcaa ttaagaacat tgttggttgg ggttatcaag
    13801 ccagaaagcc cggcgacagc agcggcaatt cttgcctcca aagatcccgc aaaaacctgg
    13861 caagaatatg aatcctctgg tggcaagctt aagctaaacg tgcctgcaaa cgtaagtaca
    13921 gaacaaatga aagtgttaag cgccaacgag aaactcatgg acgatctggg ggcaaatgtc
    13981 acaccagcga tctattacat gagtaaggaa aatacgctgc aacaggccgt ggggttgccc
    14041 gatcagaaaa cgcttaatat cattatgggg aataaataaa agataattca tggatggttg
    14101 aaatacatcc ctgcttttca accatcctaa aaaagcatga attgctcgct ctgccctaag
    14161 tggctatgct attttagaaa aacttaatcc tgagttaagc aaaaaaaata taatcaataa
    14221 aaatatatac gcaaaaaaca ttgattaagt gaatatacca tggaagaaaa atataaccgg
    14281 agtagtgtat ggccaatctc tacgacttga aaaagttcga cttaaacctt cttgtcatat
    14341 ttgagtgtat ttaccagcat ttaagcatta gcaaagctgc tgaatcacta tatatcaccc
    14401 catcagccgt gagccagtcg ttacaacgct tacgtgcgca atttaatgac cctttattca
    14461 ttcgctcagg aaaaggtatc gcgccgacga caacagggtt gaatctgcat caccatcttg
    14521 agaaaaacct caaaggcctt gaacaaacaa ttaatatagt caataagtcc gaactaaaga
    14581 aaaactttat tatttacggc cctcagctca tttcttgctc taataatagt atgctgatcc
    14641 gctgcctacg gcaagatgcc tctgtcgaga ttgagtgcca tgatatcctc atgtcagcag
    14701 agaatgctga agaactgttg gttcaccgca aagccgatct ggtgatcacc caaatgccag
    14761 tgatcagccg ttccgtcatt tgcatgccat tgcatacaat tcgcaatact ctaatttgta
    14821 gtaataagca tccgcgaatt actgacaata gcacttatga acaaattatg gcagaagagt
    14881 tcacccaatt aatatccaaa tcagcaggag ttgatgatat tcaaatggaa atagacgaga
    14941 agtttatgaa tcgcaaaatt tcatttcgtg gttcctcttt gctgacaatc attaatagca
    15001 ttgcagttac agatttactg ggaatagtcc cctatgaact ttataattcc taccgtgact
    15061 ttcttaattt aaaagagata aaactag
//
LOCUS       DAZFXT010000112        14840 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.112, whole genome shotgun sequence.
ACCESSION   DAZFXT010000112 DAZFXT010000000
VERSION     DAZFXT010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14840
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.112"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(87..794)
                     /gene="yqgA"
                     /locus_tag="ACHKW0_003718"
     CDS             complement(87..794)
                     /gene="yqgA"
                     /locus_tag="ACHKW0_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HFO7476478.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRISMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPTHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            947..1030
                     /gene="yqgH"
                     /locus_tag="ACHKW0_003719"
     CDS             947..1030
                     /gene="yqgH"
                     /locus_tag="ACHKW0_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqgH"
                     /protein_id="HFO7476479.1"
                     /translation="MHATSLHSLAYRRFARNVSLSMLLLMR"
     gene            1192..3327
                     /gene="speC"
                     /locus_tag="ACHKW0_003720"
     CDS             1192..3327
                     /gene="speC"
                     /locus_tag="ACHKW0_003720"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HFO7476480.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCQLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="ACHKW0_003721"
     CDS             complement(3376..4632)
                     /gene="nupG"
                     /locus_tag="ACHKW0_003721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HFO7476481.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="ACHKW0_003722"
     CDS             complement(4834..5913)
                     /gene="mltC"
                     /locus_tag="ACHKW0_003722"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HFO7476482.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(5978..6253)
                     /gene="yggX"
                     /locus_tag="ACHKW0_003723"
     CDS             complement(5978..6253)
                     /gene="yggX"
                     /locus_tag="ACHKW0_003723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HFO7476483.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="ACHKW0_003724"
     CDS             complement(6281..7333)
                     /gene="mutY"
                     /locus_tag="ACHKW0_003724"
                     /EC_number="3.2.2.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HFO7476484.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEMMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWAL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            7494..8213
                     /gene="trmB"
                     /locus_tag="ACHKW0_003725"
     CDS             7494..8213
                     /gene="trmB"
                     /locus_tag="ACHKW0_003725"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine(46)-N7)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HFO7476485.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            8213..8539
                     /gene="yggL"
                     /locus_tag="ACHKW0_003726"
     CDS             8213..8539
                     /gene="yggL"
                     /locus_tag="ACHKW0_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HFO7476486.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            8723..9442
                     /gene="yggN"
                     /locus_tag="ACHKW0_003727"
     CDS             8723..9442
                     /gene="yggN"
                     /locus_tag="ACHKW0_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HFO7476487.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            9618..10664
                     /gene="ansB"
                     /locus_tag="ACHKW0_003728"
     CDS             9618..10664
                     /gene="ansB"
                     /locus_tag="ACHKW0_003728"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004067 - asparaginase activity [Evidence
                     IEA];
                     GO_process: GO:0006528 - asparagine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HFO7476488.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDLATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAQALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            10781..11788
                     /gene="yggM"
                     /locus_tag="ACHKW0_003729"
     CDS             10781..11788
                     /gene="yggM"
                     /locus_tag="ACHKW0_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HFO7476489.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(11844..13145)
                     /locus_tag="ACHKW0_003730"
     CDS             complement(11844..13145)
                     /locus_tag="ACHKW0_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HFO7476490.1"
                     /translation="MDSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIA
                     AITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVN
                     VLANMMFGSISGSAVAAAAAVGGTLNPIQTKQGYDPAFSTAVNVSSCITGLLIPPSNV
                     LIVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKA
                     FLDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILE
                     SVVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGI
                     FMDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISG
                     VKIQHLIKPLLPFYAALLVALMMITYIPQISLFIPQLLGLM"
     gene            complement(13145..13648)
                     /locus_tag="ACHKW0_003731"
     CDS             complement(13145..13648)
                     /locus_tag="ACHKW0_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HFO7476491.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGLVMMFYALCFINQSIQQLK
                     QHAQEAS"
     gene            complement(13693..14679)
                     /locus_tag="ACHKW0_003732"
     CDS             complement(13693..14679)
                     /locus_tag="ACHKW0_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HFO7476492.1"
                     /translation="MKMRSFTRSLVCASLLTLVSTGVNAAEKVTLKLAHNLERSHVVH
                     QSFEELAKEVKQLSKGNMVIRIYPSSQMGNARETMELLQNGALDMTKGSASDLESFDN
                     IYAIYNLPFLFKDQAHFNKVVFGEVGKEIMDSTKDKGFFALSAYVAGTRSFYAKKPIT
                     KPEDLKGLKIRVQPSPTTIKMIELMGGSPTPISFGEVYTAMQQGVVDGAENNVPSWVQ
                     TRHIEIAKVFSEDEHASIPDFLVISTKTWNKLTPEQQQILETATKKSEAYQQKLWEKI
                     DADTRAQAKAMGGEIVKVDKAPFRAAVQPLFDDFKKDPKQAALLEKFDNAAQ"
BASE COUNT         3944 a         3536 c         3744 g         3616 t
ORIGIN      
        1 gattcgcctc gtctcgtcaa tcaaatttct tcaatttact gcagactgca caatctatca
       61 tcactttgcc gctattgcac gcattgtcag gcaaaccagg cggtccaggc ggcggaaagc
      121 ggcattgcca gaaggagtgc tggaagcatg ttgaccaccg ggaacatttt aatgccacag
      181 atgcgtaatc cggttgccag cagcaataaa ccgcctacgg cgctgaagtc tgccatcatc
      241 gacggtgtgg tcagcggtaa tatcagcgcg gcagcccacg ccagcgttaa ctggatgatc
      301 agtaatggga tactaattac cgacaccgca atacccagcg agcaggcgaa gatcatcgcc
      361 gtaaagaaat caagaaatga cttggcgatt aaaatactcg gatcgccggt catcccttcg
      421 ttcatcgccc cgaagatccc ggtgccgctg gcgcaaaaca ggacaataat cgcgacataa
      481 ttctgaataa aagattcatg cgttggcttc ttacgtgagt gacgaaacag attttgtgct
      541 ttggcgactg ctgtattgac acctttctcc agcaaacaaa tttcgccaat tagagcgcca
      601 agtagcgtag ctaaaaccat cgctggaagg ttggcgcatt tcacaaccag taaaatacca
      661 atccccagcg atgccagacc aaaaatagat gtcatggaga tgcggatacg ttccggtaag
      721 cgttggctga gcagtgcgcc gagaacgcca cccagtaaga ctgcactagc gttgataaaa
      781 gggccgatga ccacaagagt tcctgttaac tgtattgttt gattttctta ttatgaactt
      841 tttgccttgt tgatggcttg tctggtcgca aagtcctggc ttgcacgctt tagcgaaagg
      901 tgccatgatt gcgcgaattt tctcctctct gtacggagtt tgcccgatgc acgccacctc
      961 cttacattct ctcgcttatc gccgtttcgc gcgaaacgtt tccctttcta tgttactgct
     1021 catgcggtga agttaacgca cgctcactgc aggacaacag taaaatcaga gcgtttctgc
     1081 ttttactgat gtctggcggt cggagctggt gaccagtttg acccatatct catggggcag
     1141 ggttttccac cttgtccggt attcttactt ccccgaaacg ggtttgcgct tatgaaatca
     1201 atgaatattg ccgccagtag tgaactggta tcccgacttt cttctcatcg tcgcgtggtg
     1261 gcgttgggag atactgattt tacggacgtc gcggcagtcg tcattaccgc tgcggatagt
     1321 cgcagtggca ttcttgcgtt gcttaagcgc accggttttc atctaccggt gtttttgtat
     1381 tccgaacatg ctgttgaatt acctgcgggc gttacggcgg taatcaacgg caacgagcag
     1441 cagtggctgg agctggaatc cgcagcctgt cagtatgaag agaatttgct gccaccgttt
     1501 tatgacacgc tgacgcagta cgttgagatg ggcaacagca cctttgcttg ccctggacat
     1561 caacatggtg cgttttttaa aaagcatcct gccggacgcc atttttacga tttctttggt
     1621 gagaacgtct ttcgcgccga tatgtgtaac gctgacgtaa aattgggcga tctgcttatt
     1681 catgaaggat cggcgaaaga tgcgcagaaa ttcgcagcca aagtctttca tgccgataaa
     1741 acctattttg tgctgaacgg cacatcggca gcgaataaag tggtgacgaa tgcgctgtta
     1801 acgcgtggcg atctggtgct cttcgaccgt aacaaccata agtcgaatca tcacggcgcg
     1861 ctgattcagg cgggggcgac gccggtctat ctggaagctt cacgcaaccc gtttggtttc
     1921 attggcggta ttgatgcgca ctgttttaat gaagagtatc tgcgccagca aattcgcgac
     1981 gttgcgccag aaaaagccga gctgccgcgc ccgtttcgcc tggcgattat tcagctggga
     2041 acctatgacg gaactgtcta taacgcccgt caggtgatcg ataccgttgg gcatctgtgt
     2101 gattacattc tgtttgattc cgcgtgggtc ggttacgaac agtttatccc gatgatggcg
     2161 gatagctcgc cgctgctatt agaacttaac gaaaacgatc cggggatttt tgtgacccag
     2221 tcggtgcaca aacagcaggc tggattctca cagacgtcgc agatccataa aaaagataac
     2281 catatccgcg gacaggcgcg tttttgcccg cataagcggt tgaataatgc ctttatgctc
     2341 catgcttcta ccagcccgtt ctacccgctg tttgccgcgc tggacgttaa tgccaaaatt
     2401 catgaagggg agagtgggcg tcggctgtgg gctgagtgcg ttgagttggg tattgagtcg
     2461 cgcaaggcta ttcttgcgcg ctgtcagctg ttccgcccgt ttatcccgcc cgttgttgat
     2521 ggcaaattgt ggcaggatta tccgacatct gtgttagcca gcgaccgccg ttttttcagt
     2581 tttgagccgg gggcgaagtg gcacggcttt gaaggatatg ctgcggatca gtattttgtt
     2641 gatccgtgca agctgttact cactacacca ggtatcaatg ccgaaaccgg cgaatatagc
     2701 gactttggcg ttccggcgac gattctggcg cactatctgc gtgagaacgg cattgtgccg
     2761 gagaagtgcg atctcaactc cattctgttc ttattaactc cggcggaaag ccacgagaag
     2821 ctggcacaac tggtagcgat gttggcgcag tttgaacagc atattgagga tgactcgccg
     2881 ctggctgagg tgttgccgag cgtttataac aaatatccgg tgcgctatcg cgactacacc
     2941 ctgcgccagt tgtgtcagga gatgcacgat ctgtatgtca gtttcgacgt caaagaccta
     3001 caaaaagcga tgttccgcca gcagagtttc ccgtcagtgg tgatgaaccc ccaggatgcg
     3061 catagcgctt atattcgcgg tgaagtggag ttggtgcgga ttcgtgatgc cgaagggcga
     3121 attgcggcag aaggggcgtt gccttatcca cctggcgtgc tttgcgtggt acccggggaa
     3181 gtctggggtg gggcggttca acgttatttc cttgcgctgg aagaaggcgt gaatttgttg
     3241 ccgggatttt cgccggagtt gcaaggtgtc tatagcgaaa ccgatgcgga tggcatgaaa
     3301 cgattgtacg gttatgtgtt gaagtaagaa taaaaaaacg ggtcaccttc tggcgacccg
     3361 tttttctttg cgtaattagt ggctaaccgt ctgtgtgcct gtcgggacac gaacgtgttt
     3421 atatttgaac atcgccatga acgcgaaggc cagaaccacg gaataaccag cgaaaatcaa
     3481 ccatacggtc tgccagtcgg taatgccgtt ttgggtgtac atctcaacaa ctttaccgct
     3541 cacgatgccg ccgaggatac agccgaagcc gttagtcatc atcaggaaca tcccttgtgc
     3601 actggcgcga attgccgggc taacttcttt ttcgacaaac accgaaccag agatgttgaa
     3661 gaagtcgaat gcgcaaccgt aaacgatcat cgacagtacc agcagtacag taccgaacgg
     3721 agtcgggtcg ccgtaagcaa acagcgcaaa acgcaggatc cacgccacaa tactgatcat
     3781 cattacgttc ttaataccgt agcggcttaa gaagaacggg atggtcagaa tgaacagggt
     3841 ttcagagatc tgcgaaatcg acatgatgat tgacgcatgc tgcacgatga agctgctggc
     3901 aaacatcgga tctttgtcga agctgtgcag gaaggtatta ccgaacatgt tggtaatctg
     3961 cagttccgcg cccagcagca ttgagaagat gaagaagatg gccatacgct tgtttttaaa
     4021 cagcgcgaat gcatcgaggc ccagcagggt tgtccagctc tgattcgctt gctgtttagc
     4081 aaccggaata tgcggcagag tcagggtaaa cagaaccaga atggcggaaa gagctgcgcc
     4141 aatatacagc tgcatgtggc ttaattcgaa gccagacagg ctcaccaccc acattgccat
     4201 gataaagccg atggtgcccc agatacggat tggcgggaag tcagtaacga tatccatccc
     4261 ggcattttgc aggcgatagt aagagatggt gttgattaac ccaagcgttg gcatataagc
     4321 aaacgagtta atcaatatca caaggaacat cgcctccggt gtcgtgacct gtgccgccat
     4381 gaacagcgtg atagcgccaa tggtgtggca aatggcatat acccatttcg cacttaacca
     4441 tttgtcggcc acaatcccca gcagcgcagg cataaagacc gctgcgatac ccagtgagct
     4501 ataaactgcg ccaatagaag caccgtcaaa cttcagggta acaaacatat aggaaccgag
     4561 ggtcgtcagc caacttcccc acagacagaa ctgcagaaaa gagaggattt tcagctgcag
     4621 cttaagattc atgttaattt cctcacatcg tgatgcggat gagtgtttga aaaagcacat
     4681 ttgaccgtgc gagatgatgg gattctatca acttcgcgga ctggtttttt gttacctgtg
     4741 gcaaataatt gcaaacattc ccaatttgca aaatgaaatt gtgatgtgga taacattttt
     4801 gcccctgagc atcgtcaggg gcggttaatg gaattatcgg cggcggtagg atttttgcgc
     4861 ggtattcact ttataaagat aacggcgaga ttccgcagag ggatggcggg tcgtcagcgt
     4921 ctgataaaca tcgcccggcg tcatggtgtt aataatattg gcagcctgaa tcttatcatt
     4981 cgaaaagact cgcagcacgc tgcctgcgcc gccgttatag gcggtgatga cggcataacg
     5041 ccgcgatgtt gggttatcaa ttccgccgag ataaacattg ttcagcatcg ccagatacgc
     5101 ggtgccggta tcaatattgc tggcaggatc aaacaagaaa ctgcggctcg gcgtgccgga
     5161 tttcccctgc gagcggaaca catctttccc ggcagtatgt tgtaccacct gcattaatcc
     5221 cagcgcatcg gaacggctga ccgcatacgg gttaaaggaa gattcggtct gcataattgc
     5281 cagaatcagc gactcatcaa cgccatattt ccgtgacgcc tggcggacca tgccgagata
     5341 tttgtgcgca cgtttatcaa ggtggttcgg taccatgtta atggtgacgc tgtagatgat
     5401 acgcagcccg ttactgcggc tttgcagacg gtttttcagc agataatccg cgaagttgct
     5461 tgcgcgacct tcccagcgaa tcggctgccc ggtgttgtcc accacctgac cgtaaaggaa
     5521 aggttctttc gaaatcgtaa tatcatcaac gtcggaatag agatcgaccg aactcggatc
     5581 gtcacccatc agcaacgttt tgataattgc ccggcgcaaa tgcgcggcag gttctgtccc
     5641 ggcgatagtt tcgatagtaa tcgtaccgtc atcgaagttg atgtggctgc gggtctgata
     5701 ttgatcggtg tatttcacgt agtccttagg accggcgatc accacctctt tgaatcccca
     5761 gatgttctca atattgtggg caaattgccc catcagaata tcaaaaccgt tggtatcttt
     5821 gacccaggct tcgttatagg tatcgccttt tttggtcgtc gaacaggaga tgagcaacgg
     5881 cgcaatcaaa gccagcgcga gatatttttt catcattccg ggtgcgtgtt gtgtttgttt
     5941 ttatgcaagt tgccggaggc gtgctccggc actgttttta ttttttatct tccggcgtat
     6001 agccctcgat atgcacctct ttaccctcga acaggaagtt gaccatctcc tgctcaagca
     6061 gcttgcggtg ctcggcattc atcatgttga gtttcttttc attaatcagc atggtttgct
     6121 tgtgctgcca ctgcgcccag gcttctttgg agatctcgtt atagatgcgt tttcccagct
     6181 cgccggggta cagctgaaaa tcctgacctt ctgcttcacg ttgcaggaaa gtacaaaaaa
     6241 tcgttctgct cataaatcat cctctttatc gactcacgcg ctaaaccggc gcgccagtgc
     6301 gtaattgctg taacaaacgc tccacgggag ccgccaggcc aactgacggc ggttgcgcta
     6361 agttatacca gagcgcattg ccttcatcca tgcagccggt gaatgacgac acgggaagcc
     6421 acataggcac aatatctaag tggaaatggc tgaaggtatg gcgaaacgcg gtcagctgcg
     6481 tcaggttatc ggcagcaatc tgccgttgcg ccagccaatg ccgcaaactt tcttcgtcgg
     6541 caaactgcgg gaaacagtat aaaccgcccc acaatccgct cggcggacgc tgcgccagca
     6601 atacttcatc ttcgtgctgt aatagcaaaa agtagccggt gcgctccggc agcgtctgtt
     6661 tcggtttttt gcccggataa agcgcccagc tattgttggc ggcggcaata catccgtttt
     6721 gtagcggaca gagcgaacat ttcggtttcg agcgcgtaca aatcatcgca cccaaatcca
     6781 tcatcgcctg attaaaccgt tccacgccaa ccgcgggcgt cacctgctcg ctcaaactcc
     6841 ataatttatt ctcgacctct tttttcccag gccagccgct tacagcatag cagcgcgcca
     6901 gcacgcgttt gacgttaccg tcgagaatcg gaaagtgctt acccagagaa agcgagagaa
     6961 tcgctcctgc ggtggaacgc ccgacgcccg gtaacgccgc gacttcttca aaggtttccg
     7021 ggaatttacc gccgtgtaag gtcgccactt gttgtgccgc tttatgcaga ttgcgcgcgc
     7081 gggcgtaata gccaagcccg gtccacaagt ggagaacttc gtcgagcggc gcattggcga
     7141 gatcggtcac cgtcgggaag cgcgccataa agcgttcaaa ataggggata acggtcgcaa
     7201 cctgagtttg ttgcaacatc atttctgaga gccatacttt gtagggcgtc ttgtcaattt
     7261 gccagggcag cgtttttcgc ccgtatttat cgtaccagtc cagaacctgg gctgaaaatt
     7321 gcgacgcttg catggtcacc gaattcactg ttgttggggg caagattgca gcacagcgac
     7381 ggcagggtgt aaaccggaac tttccgcagc cacggcctta tcatgcttgc atccggcaac
     7441 taactttgga taatgcccgt tttcagaaca ctttcacaag cgactaaatc tttatgaaaa
     7501 acgacgtcat ttcaccggaa tttgatgaaa acggccgccc actgcgccgt atccgtagtt
     7561 ttgtgcgccg ccaggggcga ctgaccaaag gccaggaaca tgcgctggaa aactactggc
     7621 cggtgatggg cgttgagttc agcgaagata tgctggattt ccccgcgctt tttggccgtg
     7681 aagcgccggt gacgcttgag attggttttg gcatgggggc gtcgctggtg gcaatggcta
     7741 aagatcgccc tgagcaggac ttcctcggca ttgaagtgca ttcaccgggc gttggtgcgt
     7801 gcctggcttc tgcgcatgaa gaaggtttaa gcaacctgcg agtgatgtgt cacgatgcgg
     7861 ttgaagtgct gcataaaatg attcctgaca attcattgcg catggtgcag ctctttttcc
     7921 ctgacccgtg gcacaaagcg cgccataata aacgccgtat cgtacaggtg ccgtttgccg
     7981 aactggtaaa aagcaaactg cagctggggg gcgtattcca tatggcgacc gactgggaac
     8041 cttatgcgga acatatgctt gaagtgatgt cttctattga cggttataaa aacctgtcag
     8101 agagcaatga ttacgtaccg cgtccggcat cacgtccggt gacgaaattt gaacaacgtg
     8161 gtcatcgtct tggtcacgga gtatgggact taatgttcga gagggtgaaa taatggcaaa
     8221 gaaccgtagc cgtcgtctgc gtaaaaaaat gcacatcgac gaattccagg aattaggatt
     8281 ttcggtggca tggcgattcc cggaaggtac atcggaagaa cagattgata aaaccgttga
     8341 tgattttatt aacgaggtta tcgaaccgaa caaactggcc tttgacggca gcggttatct
     8401 ggcctgggaa ggtctgatct gcatgcagga aatcggcaaa tgcaccgaag aacatcaggc
     8461 gattgtgcgt aagtggctgg aagagcgcaa actggatgag gtacgcacca gcgaactttt
     8521 cgacgtttgg tgggactaag aaagcatacg ggcgatgaca aatgcaaaac tgcctgatgc
     8581 gctacgctta tcaggcctgg aaagatgcac gatcgagtag gcgggataag gtgtttacgc
     8641 cgcatccggc atggaaaacg cgtactttgt tatcaatctg gggccagcaa atgctggcct
     8701 gattttttct tgagggaaga ctatgatgcg caaaatgctg ctggcggcag cactttcagt
     8761 gacggcaatg accgctcacg ccgactacca gtgcagcgtc acgccgcgtg acgatgtgat
     8821 tgtcagcccg caaaccgtgc aggtgaaggg cgaaaacggc aatctggtga tcacgccaga
     8881 cggcaacgtg atgtataacg gtaagcaata ttccctgaat gccgcccagc gcgagcaggc
     8941 gaaggattat caggctgaac tacgcagcac gctgccgtgg attgatgaag gcgcgaaaag
     9001 ccgcgtcgaa aaagcccgta ttgcgctgga taaaattatc gttcaggaga tgggcgaaag
     9061 cagcaaaatg cgcagccgtc tgaccaaact tgatgcgcag ctgaaagagc agatgaaccg
     9121 cattatcgaa acgcgcagcg atggcctgac gtttcactat aaagccattg atcaggttcg
     9181 cgccgaaggc cagcaattag tgaatcaggc aatgggtgga attttacagg acagcattaa
     9241 tgaaatgggc gcgaaagcgg tgctgaaaag cggcggtaac ccattacaga atgtgctggg
     9301 aagcctgggg gggctgcaat cctcaatcca aaccgagtgg aaaaagcagg aaaaagattt
     9361 ccagcagttt ggcaaagatg tttgtagccg cgttgtgact ctggaagata gccgcaaagc
     9421 cctggtcggg aatttaaaat aatcctctat tttaagacgg cataatactt ttttatgccg
     9481 tttaattctt cgttttgtta cctgcctcta actttgtaga tctccaaaat atattcacgt
     9541 tgtaaattgt ttaacgtcaa atttcccata cagagctaag ggataatgcg tagcgttcac
     9601 gtaactggag gaatgaaatg gagtttttca aaaagacggc acttgccgca ctggttatgg
     9661 gttttagtgg tgcagcattg gcattaccca atatcaccat tttagcaacc ggcgggacca
     9721 ttgccggtgg tggtgactcc gcaaccaaat ctaactacac agcgggtaaa gttggcgtag
     9781 aaaatctggt taatgcggtg ccgcaactaa aagacattgc gaacgttaaa ggcgagcagg
     9841 tagtgaatat cggctcccag gacatgaacg ataatgtctg gctgacactg gcgaaaaaaa
     9901 ttaacaccga ctgcgataaa accgacggct tcgtcattac ccacggtacc gacacgatgg
     9961 aagaaaccgc ttacttcctc gacctgacgg tgaaatgcga caaaccggtg gtgatggtcg
    10021 gcgcaatgcg cccgtccacg tccatgagcg cagacggtcc attcaacctg tataacgcgg
    10081 tagtgaccgc agctgataaa gcctccgcta atcgtggcgt gctggtggtg atgaacgaca
    10141 ccgtactgga cggtcgcgat gtcaccaaaa ccaacaccac cgacctagcg accttcaagt
    10201 ctgttaacta cggtcctctg ggatacattc acaacggtaa gattgactac caacgtaccc
    10261 cggcacgtaa gcacaccagc gatacgccat tcgatgtctc taagctgaat gagctgccga
    10321 aagttggcat cgtttataac tacgctaacg catccgatct tccggctcaa gcactggtag
    10381 atgcgggcta tgatggcatc gttagcgctg gtgtgggtaa tggtaacctg tataaatccg
    10441 tgttcgacac cctggcaacc gccgcgaaaa acggcactgc agtagtgcgt tcttcccgcg
    10501 taccgacggg tgctaccact caggatgctg aagtggatga tgcgaaatac ggcttcgtcg
    10561 cctctggcac gctgaacccg caaaaagcgc gcgtcctgct gcagctggct ctgacgcaaa
    10621 ccaaagatcc gcagcagatc cagcagatct tcaatcagta ctaatcacct cgccccggta
    10681 tcgtgccggg gctttttcac ttcagactta cgtccattgc caattttcat taccctaatg
    10741 ataatcaccg gaataaatta ttccgcgtga ggtttttcgg gtgaaaaagc aatggattgt
    10801 tggcacggca ctgcttatgt tgatgactgg taatgcctgg gcagatggcg aaccgccaac
    10861 tgaaaatatc ttaaaagatc aattcaaaaa gcagtatcac ggcattctca agcttgatgc
    10921 tatcacctta aaaaatcttg atgctaaggg taatcaggcc acctggtcag cggaaggcga
    10981 tgtctcttcc agtgacgatc tctatacctg ggtcggtcag ttggcagatt acgaactgct
    11041 cgaacagacc tggacgaaag ataaaccggt aaaattctcg gcgatgttaa ccagtaaagg
    11101 aacgcccgcg tctggctggt cggtgaactt ttactctttt caggcggcag ccagcgatcg
    11161 tgggcgggtg gtcgacgata tcaaaacgaa taataaatat ctgatcgtga atagcgaaga
    11221 tttcaattat cgctttagtc agcttgaatc tgcgttgaat acccagaaga actcgattcc
    11281 tgcgctggaa aaggaagtga aagcgcttga taagcaaatg gtggcggcgc agaaagcagc
    11341 ggatgcatat tggggaaaag atgcaaacgg taagcaaatg acccgcgaag aagcctttaa
    11401 aaaaattcac caacagcgtg atgagtttaa taaacagaac gatagcgagg cgttcgcggt
    11461 taaatatgac aaagaggttt atcaaccggc gattgcggca tgtcataaac agagtgaaga
    11521 gtgttatgaa gtgccgattc agcagaaacg agatttcgat atcaacgaac aacggcgaca
    11581 gacttttctg caatcacaaa aactcagccg taaattgcag gatgactggg taacgctcga
    11641 aaaagggcaa tatccgttaa cgatgaaagt ttcagaaata aacagtaaaa aagtcgcgat
    11701 tttgatgaaa attgacgata tcaaccaagc taatgaacgc tggaaaaaag ataccgaaca
    11761 actgcggcgc aatggggtga ttaagtaagt ttgatatagt gcctttgccg gataatataa
    11821 aaagttatcc ggcaaattat tttttacatt agccctagta gctgtggaat gaacaaggat
    11881 atttgcggaa tgtaagtgat catcatcaac gctactagca atgcagcata aaatggcagc
    11941 aatggtttga ttaaatgttg aatcttcaca cccgagatag aacaaccaac aaatagcgca
    12001 ctacccaccg gcggtgttaa cagcccaata caaaggttgg ccaccatcat aataccgaaa
    12061 tgtactgggt ccattcccag ctcttgtgca atcggcaaga agatcggtgt aaagattagt
    12121 acagccggtg tcatgtccat aaagatgccg acaattaata agacgatatt aatcagcagc
    12181 aatatgatca gtgggttatc agaaatcccc atcaatgcgt cgctgatcat ataaggaata
    12241 tcagcattag tcattgccca ggacatgccc acggaaaaac cgatcaacag taatacaatc
    12301 gaagtggtaa caaccgactc cagaatcagt ttgggtaaat ggcgccattt tacttcccgg
    12361 taaatcagca ccgagagaat aaatgtgtag acaacggcaa ttgctgacgc ttcagttgcg
    12421 gtaaaaatac cgcccagaat gccgcccatc acaatgaaca ccagaagcaa acttggtaag
    12481 gcatccagaa acgctttaca ggcctgagca aaggttgctc tttctgaaag cgggtagcca
    12541 cggcgtttag caataatgcc gcagactatc attaccgcaa gacccattaa gatccctggc
    12601 aaatatcccg ccataaatag cgatgctaca gaaacgccgc cagctgtcag ggaaaacaca
    12661 attaacacgt ttgagggcgg tatcagtaat ccagtaatac aagatgagac gtttacagcg
    12721 gtagaaaatg ccggatcata accttgtttg gtctgaatgg gattcagtgt gccaccaacg
    12781 gcagcagctg ccgcaacggc tgaaccagaa atggaaccaa acatcatatt cgccaggacg
    12841 ttcacatgac caagtgaacc aggaacccga ccaaccatta cctgggcgag gttaatcaaa
    12901 cgtatcgcaa taccgccgct gttcatcagc gttccggcaa agatgaaaaa ggggattgcc
    12961 aatagggcaa agttatccag tccattagcc agtcgctgcg aaacagtgat tgccgcgata
    13021 tcccagggaa acatcaaaag cattgaagct acagtggcaa taccgattga gaacgaaatg
    13081 ggcacaccaa taaagaccaa cacaaaaaaa cagccaaaca gtgtaatcgc gatataacta
    13141 tccattaact ggcctcctgc gcatgttgtt tcagttgttg aatgctttga ttaataaaac
    13201 acaaggcata aaacatcatc accaatccac ttaatggcag aatgatatat acgtatccca
    13261 tcggtatttg catcgcggca gatacctgtg atgttgccag cgttttatca attaatttca
    13321 ggccaccagt cacaatgacc gaaccggcaa aacctaaaat aagaacgtta atgactatac
    13381 taagaagtaa ttgcttgcgc ttattcaggg cgagagcaaa taaatcgata gagagatgtc
    13441 tctgcgcgcc gacggtatat gcagcaccta ataaccctac ccagatcatc aaaaatcgtg
    13501 ccagctcatc ggttaatgta cttggctggt taagtacgta gcggctaaat acttgccaga
    13561 ctacgcatac aaccagagca agcatgacgg cgacagaaaa cgcggcaatg acgcggtcta
    13621 ccgcaagttt caggcggtta aatatcattg taagcatcct ggaaaatcag ggccagagac
    13681 tggcccggaa tattattgag cagcgttgtc gaacttctct aacaaagcag cttgttttgg
    13741 atcttttttg aagtcgtcaa acagaggctg aactgcggca cggaatggtg ctttatccac
    13801 ttttacaatt tcacccccca tagctttcgc ctgagcacgt gtgtcagcgt caattttttc
    13861 ccacaatttt tgttgataag cttcggattt cttcgttgct gtttcgagga tttgttgctg
    13921 ttctggggtt aatttattcc atgttttggt ggagataacc aaaaagtcag gaattgaggc
    13981 gtgttcatct tcagaaaaaa ctttggcaat ttcaatatgt cgagtctgca cccaggaagg
    14041 cacgttattt tctgcgccat ctactacacc ttgctgcatc gcagtataaa cttcgccaaa
    14101 tgaaattgga gtgggtgaac cacccatcag ttcaatcatt ttgatggtgg tcggacttgg
    14161 ctgaacacga attttcagcc ctttaagatc ttctggctta gtaatcggtt ttttcgcata
    14221 gaaactgcgt gtacccgcta cataagctga taaagcaaaa aagcctttgt cttttgttga
    14281 gtccataatt tctttaccaa cttcaccaaa aacaactttg ttgaagtgag cctgatcttt
    14341 aaataagaac ggcaaattat agatagcata aatattatca aaagactcca gatcactggc
    14401 ggaacctttg gtcatatcca gagcgccatt ttgcaacaac tccatagttt ctcgcgcatt
    14461 acccatctgg ctgctgggat aaatacggat caccatatta ccttttgaca gctgttttac
    14521 ctctttcgcc agctcttcaa aactctgatg tacaacatga cttctttcca ggttgtgtgc
    14581 taactttaac gtcacttttt ctgcggcgtt tacgccagtg ctaaccagag tcagcaagga
    14641 tgcgcagact aatgagcggg taaatgacct cattttcata aaaaaactcc ctgttgaagg
    14701 atggaaattg gttgatatct aattgtgctt atcagtgctt tgtgcgacaa attggttaac
    14761 ctgtctgaca acttgtctat gtttatcaca acttgaaata aagtcagaaa ttaaatatta
    14821 aatttgtgcg ccaggttgta
//
LOCUS       DAZFXT010000113        14512 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.113, whole genome shotgun sequence.
ACCESSION   DAZFXT010000113 DAZFXT010000000
VERSION     DAZFXT010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.113"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..444
                     /locus_tag="ACHKW0_003733"
     CDS             88..444
                     /locus_tag="ACHKW0_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7476493.1"
                     /translation="MQWYLAVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLEFPFLSLIYLAATIIPVIALCVRRLHDTDRSGAWALLYLVPIIGWLVLFVFACLEG
                     NSGSNRYGNDPKFGSN"
     gene            complement(495..1391)
                     /gene="yhaJ"
                     /locus_tag="ACHKW0_003734"
     CDS             complement(495..1391)
                     /gene="yhaJ"
                     /locus_tag="ACHKW0_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HFO7476494.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFSGK"
     gene            1496..2197
                     /locus_tag="ACHKW0_003735"
     CDS             1496..2197
                     /locus_tag="ACHKW0_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HFO7476495.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2220..2384
                     /gene="yhaL"
                     /locus_tag="ACHKW0_003736"
     CDS             2220..2384
                     /gene="yhaL"
                     /locus_tag="ACHKW0_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaL"
                     /protein_id="HFO7476496.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2596..3906)
                     /gene="cyuA"
                     /locus_tag="ACHKW0_003737"
     CDS             complement(2596..3906)
                     /gene="cyuA"
                     /locus_tag="ACHKW0_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfidase"
                     /protein_id="HFO7476497.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3934..5265)
                     /locus_tag="ACHKW0_003738"
     CDS             complement(3934..5265)
                     /locus_tag="ACHKW0_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HFO7476498.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5540..6904)
                     /gene="tdcG"
                     /locus_tag="ACHKW0_003739"
     CDS             complement(5540..6904)
                     /gene="tdcG"
                     /locus_tag="ACHKW0_003739"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7476499.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6976..7365)
                     /locus_tag="ACHKW0_003740"
     CDS             complement(6976..7365)
                     /locus_tag="ACHKW0_003740"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HFO7476500.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7379..9673)
                     /gene="tdcE"
                     /locus_tag="ACHKW0_003741"
     CDS             complement(7379..9673)
                     /gene="tdcE"
                     /locus_tag="ACHKW0_003741"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HFO7476501.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9707..10915)
                     /gene="tdcD"
                     /locus_tag="ACHKW0_003742"
     CDS             complement(9707..10915)
                     /gene="tdcD"
                     /locus_tag="ACHKW0_003742"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006082 - organic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HFO7476502.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10941..12272)
                     /gene="tdcC"
                     /locus_tag="ACHKW0_003743"
     CDS             complement(10941..12272)
                     /gene="tdcC"
                     /locus_tag="ACHKW0_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HFO7476503.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12294..13283)
                     /gene="tdcB"
                     /locus_tag="ACHKW0_003744"
     CDS             complement(12294..13283)
                     /gene="tdcB"
                     /locus_tag="ACHKW0_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HFO7476504.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLIDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13382..14320)
                     /gene="tdcA"
                     /locus_tag="ACHKW0_003745"
     CDS             complement(13382..14320)
                     /gene="tdcA"
                     /locus_tag="ACHKW0_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HFO7476505.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESGFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
BASE COUNT         3784 a         3882 c         3376 g         3470 t
ORIGIN      
        1 tataacatcc gaaaaagaaa gatacgaata gcatttacac aatacccgga atagactttc
       61 gtcactttta attaaaggga tgtttttatg cagtggtatt tagccgtatt aaaaaattat
      121 gttggtttct ctggtcgcgc acgtcgtaaa gagtactgga tgtttactct gattaacgcc
      181 atcgtcggcg ctattatcaa tgtcattcaa ttgattttag gtttagagtt tccattcctt
      241 tctctcattt acctggcagc aacaatcatt ccggtcattg ctctgtgtgt tcgtcgtctg
      301 cacgatactg accgttcagg tgcgtgggcg cttctctatc tggtaccgat cattggctgg
      361 ttagttctgt ttgtatttgc ctgtttagaa ggcaattctg gcagcaatcg ttacggaaac
      421 gatcctaagt ttggttcaaa ttaatcttag aattggggcg acatttcgcc cctttttatt
      481 aataataaca aatattattt tccgctaaaa agtttgggaa tttcccgcag acaccaggat
      541 ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc gctggtcgat
      601 tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat cggatacggc
      661 atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat cgtgctcacc
      721 gttaagcgcg gctgtttgtc cagcagctgt acggtcaaca ccgggcgctc acgagcggta
      781 tccgctaccg caattccacg atatttcacg cgcgtcactt cagataacgg ttccggctcc
      841 tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaaggtata gagcttgcgc
      901 gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacaat atccgcccgc
      961 ccctgctcca gtcgttccca cgcccccgcc agcacttcgg tgatgattgc cagttgggta
     1021 ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg taccagcgcc
     1081 tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gggcttccgc atcggtagtc
     1141 agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct cccgacattg
     1201 gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc ttcttccagt
     1261 ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc cgccgccgca
     1321 aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt taatgccctt
     1381 tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa tatctggcta
     1441 acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa gaattatgat
     1501 tactacccga actgccagac agtgtggaca agcagactac ggatggttgc aggcccggta
     1561 tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt
     1621 gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt
     1681 cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg aaggcaatca
     1741 tgttcaggcc agcgccggtg aggcgttgct gctctctacc cagccgggtg tcagctacag
     1801 cgaacacaat ctcagcaaag acaaaccgtt aacacgaatg cagctttggc tggacgcctg
     1861 cccgcagcga gagaatccgc tgattcaaaa actggcgctt aatatgggca agcagcaatt
     1921 aatcgcctcg ccagagggta cgatgggaag cctgcaatta cgccagcaag tgtggctgca
     1981 ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta
     2041 tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga aagcggcgct
     2101 gacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg ttgccgattc
     2161 cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg gagatgatga
     2221 tgagtaaaaa atcggccaaa aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg
     2281 ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa gccatcgtcg
     2341 cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag gttgccggat
     2401 gcggcgaaaa cgccttatcc ggcctacaaa atccaacaac attaacgaat taaacaaccc
     2461 gtaggccgga taagatgcgc cagcatcgca tccggcatca ggtacaaaac acaacaacat
     2521 caacaagttg agcaccccgt aggccggata aggtgcgcca gcatcgcatc cggcacaata
     2581 ctcaaaacct ggcgtttatc tggccttgct cgccataatc tcgataatct gccgatccgt
     2641 ttgctgcatc gaatggcttg ctaacgcaca caggttggca atcgactgct caacatcatg
     2701 cgccacgatc ccttcattgc cggtcacggc ggtatcatcc agcgccatta acaccgcttt
     2761 ccacgcagcc gaagcactgg tcgaaacctt catcgcgcag ctgttcgacg caccatcgca
     2821 aatcatgccg ctgacatcgc cgatcatact gctgatcgcc atcgagatgg tttcataacg
     2881 cccatccacc agccatgcca tcccggcggc ggcccccatt gctgcggtcg ttgcggcaca
     2941 aagtgcagac aaacgcggta actggttatg gatgtaaatt gcgctcaaat gagaaagcat
     3001 cagcgcacgc gccagccgtt catcatccgc tccgaagtgt tctgctacca ccaccacagg
     3061 cattgttgcg gtgatcccct ggttacccga gccggagtta ctcatagccg gaagcgtagc
     3121 gccgcccata cgcgcatcgg atgccgcgct ggtacgaatc acaatggatg aagagagatc
     3181 tttcgccagc aaaccgcgct cgcactgttt ttccagcgtc gcgccaatat gcagccccca
     3241 cttaccgctc aaaccttcct gcgataacgc acaatttagc ttcgcggaat cgagaataaa
     3301 gcggatcgcc gcaaacggga cttcattgac gaacttcagg atctcagcca gcgtcgttct
     3361 ggaaagcacc gtcagcggag actcttgctc gccctctgcc acacacgcct gctgggtaaa
     3421 caccacacca ttgtgcgtct caatatgcac aatgttggta tgcccgccga cgatggtgac
     3481 acacgcccac ttctcaccgt tccagacttt ggcgcgtgag aagaggattt cattgcaagg
     3541 ttcctggatc ttaacggaga ctttccccgc cgccagcagt gctttggcat cggcaattgc
     3601 ctgcgcagtt gcgtctttca gcacttccag cccggcgttg gcatttccac ctaacgcccc
     3661 cagcgccgcc gcaatcggca gccccaccat tcccgtgccg ggaacggtga cgcccagacc
     3721 gttcttcatc agatttggcg aaacccaggc ttctacacgt tcaaccggac cttccagttc
     3781 tgccgcagca accgccgccg ccagcgccag tgaaatcggt tcagtacatc ccagcgccgg
     3841 ttttacttcc tcctgaacgg cgaggatgta acgctgccat aacggattta aagtcgaatc
     3901 aaacatatta aaaaccttaa aatttcaggt aaatcaagaa aatgccagga acggagaaac
     3961 acaaagcaac aaaccagtga cgataatcag gtacagagac atccctttgt atttgtgcaa
     4021 tgccggtact ttgtaaacca gccacgccgg gatcaggcac cctaccatgc cgaaaatcgg
     4081 gctacagata gaggtgaagc tcaacaccgg tgcgttcagt acgatggcgc tccaggccag
     4141 caaaatggcg aaaatcatga tgccgcgctg aacaagattt tcgttaatct tctcggcagg
     4201 catcttgcga cgcaggatgt tcattacgat cccttgcgtt gcttcgcgaa agcctaaata
     4261 gacgccaaag aatgcagtca tgacagcaaa gatattgaga atgacgctga ccactttcac
     4321 ccacgctgcg ccgtcaccgc taataaactg tgcggcaatc gccagcgcgg aaatattctg
     4381 ctcataggct tttaccgctt cgtcatgtcc catcgccagc gtgaacgaca cggcgtagaa
     4441 aaagacggtg acaaacaaaa tgccaaacgc gatattcatt gcccgcaatg ctttatgccg
     4501 cgccacttca attgattttt cccgcgagcg ataagagatc accatcgggc ttaacgtctg
     4561 gataaacaga atcgacgtca gggtaaacgg cagcgtaata atggcgtttt tcaccagcag
     4621 ccccagcggc ggtagcgaac cgacgttata cagatgccac attcccacca tcgacacgcc
     4681 cagcgccgcg accaccagca gcttggtcag caccatgccg gtcgaaattt tgaataacaa
     4741 tttctcgccg cgtgaagaga tcgccaccag aatgcaaatc agtaccagac cataaaaggg
     4801 actgtctgac agcaaccctt ccgtcacgcc gaaggtatgc agataggaag cactatcgtt
     4861 ggtgatggcg gtggaataga cgaacatcca aatcaccagc attacgaaat agagcgcacc
     4921 taacaggatg ccccagtttt tacctaaata accgctaatg acactcgggt aatctttaca
     4981 ttctggtgat tctgccagcg tattaataaa caaccgctga aacagataca ttgccgggta
     5041 accaatcacc gatgagagca aaaataccca caatcccatc aaaccgacct gcaccgggag
     5101 aaaaacaatc cccgcgccaa tcgccatccc gatactcata atcacccagc cggtgtcagt
     5161 actgtcgaat ttgatcgctt ctcgccactc gctctcactc attccagcac gacccgccgg
     5221 ggtcgaagcg tctgcaatga cgcctttatt cgatgcaatt tccataattt ctcgctcaat
     5281 attttgtagg gcttattatt tttttccgag ccgcatcaag gcgatatgcg gtctacgttt
     5341 ttgcaggcga gttattagaa taaagaaatg atacgcgcga ggtcagagaa aatcttcaca
     5401 atcaagactt tgaaataacg gagatggata agaattttct actaaattaa tcgcaagaga
     5461 atattcattc tctatttgcg gcagatcaca aaaaaaaggt gcacatttgt gcacccaagg
     5521 atgaaagctg acagcaatgt cagccgcaga ccactttaat ggccagtcct ccgcgtgatg
     5581 tttcgcggta tttatcgttc atatctttgc cggtttcata catcgtctcg atcactttat
     5641 cgagtgaaac acgcggtgcc gaggtgcggc gcatcgccat ccgcgcggcg tttactgctt
     5701 tcacggcatt aatggcatta cgttcaatgc acgggatttg tacctgtccg gcaaccggat
     5761 cgcaggtcag cccaaggtta tgctccatcg cgatttccgc cgcattgcat acctgcgccg
     5821 gactgccgcc cagtagttca gttaaccctg ccgccgccat tgaacaggcc acgccaatct
     5881 ccccctgaca gccgacctcc gcgccagaga tggaggcgtt cattttatac agcgcgccaa
     5941 tagcccccgc tgccagaaaa tagcgggcaa ttgaccgctc gtttaccgga cgacggaact
     6001 tatcgtaata agccagcact gccggaataa tgccgcacgc accgttagtc ggtgccgtta
     6061 ccacgcgccc gccagctgcg ttttcttcac taaccgccag cgcgtacatg ttgatccagt
     6121 cgatgacatt catcggatcg ttagagatgt tatcgctgga aaccagctga cgacgcagcg
     6181 ctacggcacg acgcggcaca ttgagcggac caggcagcac gccttcagtg ttcatcccgc
     6241 gttcaatacc gtcatgcatc acttgccaga tacgggcaaa ccctgcgtca atttccgctt
     6301 tgctgcgcag cgccagctcg ttgtgcatca tcaggccaga tatagacagg ccgttgtaat
     6361 cacacatttt cagcagttca cctgctgagt ggaaatcgta aggtacggac gtttcgacat
     6421 cgtgcgacag gccgaagtgt tcttcttcga caataaaccc gccgccgaca gagtaatagg
     6481 ttttacttaa tagcgcttcc tgtcccttcc aggcagtgat ccgcattccg ttctcatggc
     6541 gaggcaacat ttcgggatgg aagataatgt tctttgctac aggaaaatca acaatatgcg
     6601 caccagatgc cactggcagc cgcccgctgc gcgttactaa ctctataaat gcagggatct
     6661 catcaatgac aacatcctgc ggactgtttc ctgccagtcc catgataatg gcgacatccg
     6721 tggcatggcc tttgcccgtc agtgacaacg acccatacag atcgaccaca atatggctcg
     6781 tcgcggttaa taagccgcta ctttccagcc gatcaataaa actttttccg gcattcattg
     6841 gccccacggt atgcgaactg gagggaccaa tcccaatttt gaaaatatcg aatgcactaa
     6901 tcatatccac accctcggat tgccgttcag tgaagtggag cggaacgacc ttgcgaccgt
     6961 cccgctcacg aggctttacg cactacgtac tgcgatagct tcaatttcca gcttcacatc
     7021 tttcggcaaa cgcgcaacct gcacacagct ccgggtcggg taggtcgcct gatgctcatc
     7081 gaagaactgc ttatagactt cattgatggt ggcaaaatca ttcagatcgg tgatgaacac
     7141 ggtcatcttg atgatatcgc ccacgctcag cccggcagca accacgatcg ctttgacgtt
     7201 ttcgaggctt aaacgcgcct gatcttgcac atcagccggg atctcaccgg tctgcgggca
     7261 aaccggaatt tgcccggagg tgaagaccat gctgcctaaa tcaacgccct gaacataagg
     7321 gccgattgcg cctggggcac gttgcgtttc gataatcttt ttcatacatc ctccggcgtc
     7381 agagcgcctg ggtaaaagta cgtgaaataa catcctgttg ctgttcacgg gtcagtgcgt
     7441 tgaagcgcac ggcgtagcca gagacacgga ttgtcaggtt aggatatttt tccgggtgct
     7501 cgatggcatc cagcagcatt tcccgattca ttacgttgac gttgaggtgt tgaccgcctt
     7561 cgacatccgc ttcgtggtgg aaatacccat ccagcaggcc gacaaggttg gttttacgta
     7621 ctggatcttc tttgcccagc gccgcaggaa cgattgagaa ggtgtacgag atcccatctt
     7681 tggcgtaggt gaacggcagt ttcgccaccg acgtcaatga ggccacggca cctttgcggt
     7741 cacgaccatg catcgggtta gcgcccggcg cgaacggtgt tccggcgcga cgaccgtccg
     7801 gcgtattacc ggttttctgg ccgtacacca cgttagaagt gatagtcaga atcgactggg
     7861 tagggacggc gttgcgatag gttggcagcg ctttaatttt cttcataaag cgttcaacca
     7921 ggtcgcaggc aatgctgtct acgcgctcgt cgttgttgcc gtactgcgga tattcaccgt
     7981 cgatttcaaa gtccaccgcc aggccgtttt cgtcacggat tggtttcacg cgggcatatt
     8041 tgatggcaga cagggagtcc gtcgccaccg acaggcccgc gatgccgcat gccatagtgc
     8101 gatagacatc acgatcgtgc agcgccatca gcgaagcttc gtagctgtac ttgtcgtgca
     8161 tgtagtgaat gatattcagc gcgctgatgt actgcaccgc cagccagtcc atgaagtgat
     8221 cgaggctgtc catcactttg tcgtaatcca gcacgtcgtc catcagcggt gctgttttcg
     8281 gcccgacctg aatcttcagc ttctcgtcca ccccgccgtt aattgcgtag agcagcgttt
     8341 tcgccaggtt agcgcgtgca ccaaagaact gcatttgctt accaatcacc attgggctga
     8401 cgcagcaggc aatcgcgtaa tcgtcgctgt tgaagtcagt acgcatcaga tcgtcatttt
     8461 catactgcaa ggaagaggtg acgatcgaca cctgcgcggc atattttttg aaggcaatcg
     8521 gtaattcttc cgaccaaaga atggtcaggt taggttccgg tgccggcccc atagtgtgca
     8581 gggtgtgcaa atagcggaag gagtttttgg tcaccagcgt acgaccgtcc agccccatcc
     8641 cgccgatcac ttccgtcgcc cagattgggt cgccggagaa cagcgaatca aattccggtg
     8701 tacgcaggaa gcgtaccata cggatcttca tgatgaagtg atcgatcagt tcctgtgcct
     8761 gctgctcatt gagtacgcca gctttaaagt cgcgctcaat gtagatatcg aggaacgatg
     8821 ccgtgcggcc cagcgacatc gcgccgccat tttgcgattt cactgccgcc agataagcga
     8881 agtagagcca ctgcaccgct tcctgcgcat tctgcgccgg gcgagagata tcaaagccat
     8941 atttcgccgc catttcctga atctgcaaca gcgcatgacg atgctctgcc agctcctcac
     9001 gcagacggat ggtggcttcc agatcctcgc ctttttccag acgagactgg agatcggcaa
     9061 attgcagttc gcgttcacgt accagataac tgatgccata cagcgctacg cggcgatagt
     9121 caccgataat gcgcccacgg ccatagccat ctggtaaacc ggtcagcacg ccagatttac
     9181 ggcagcgcag catatccggt gagtaaacat caaatacgcc ctggttatgg gttttacgca
     9241 gatcggtaaa cagatattca aattcactgt ccatttcacg gccataggcg tggaatgaac
     9301 ttttaatcat attaatgcca ccgaacgggt gtagcgcacg tttcaacggc gcatccgttt
     9361 gcaggccaac aattttttcc agcggctggt taatatatcc cgcatcatga gcggtaattg
     9421 tggtggcaat attggtatcg aaatcaaccg gcgcgtgggt tgcattttcg atacggatgc
     9481 cttccattac tttttcccac aattccgtgg tggcaggcgt cgcttcggcg aggaaagatt
     9541 catcgccttc atacggtgta tagttatgtt gaataaaatc gcggacatta atttcgtttt
     9601 tccagtccgt acctttaaag ccaagccatg cgtcggcgta cagcttatcg ctggtatcaa
     9661 tatctacctt catgaaaaat aatctctcta caatacttca actaaattat gcaaattctg
     9721 cgggcgcgtt aactttgcct aaatgaatgg catccaaagc aatcattttt tcttcgttag
     9781 tcggaataac ggcacaaatg acacgcgcat tttcactgga aacaattcgc tcaccacagg
     9841 agttagagcg attattcatt tctgtatcaa tctctacgcc taatacagcc aaatgttcca
     9901 tgaccagacg acgaattaag cttgaattct ctcctattcc gccggtgaat ataattccat
     9961 ccaggcgacg taatgaagct gcgtgtccgg caatatgacg ggcaattcgg tgaacaaagg
    10021 ttttaattgc cagttgcgcg cgttcgtgac cttcatgcca ggctttttcc agaacacgta
    10081 aatccgaaga aagaccggaa atacctaata atcccgactc tttattcact acgcgttcca
    10141 ggtcacccag gctctggttg gtttggctgg cgacccagga catcgcacca aagtcgacat
    10201 cgccactgcg ggtacccatc atcaagcctt ccagcggcgt cattcccatt gaagtatcaa
    10261 cactctgacc gttgcgaacc gcgcagattg acgcgccatt gccaagatgc gccacaacca
    10321 ggccggagtc atcttccgcc agattcagca gcgaatgtgc gcgctgggaa acatagcggt
    10381 gcgacgtgcc gtggaaacca taacggcgta cacctaactc ttcataatat ttccacggca
    10441 ggccgtataa ataagcttcc ggagccatcg tctggtggaa actggtatca aataccgcca
    10501 cctgagttac gcccggaaat aattgctgcg ccgattcaat accacttaaa ttggcgtaat
    10561 tatgcagggg tgccagtgga gaaacgcgac ggatattatc aatgacttca tcggtaataa
    10621 tggcggactc ggtaaaaata ctgccgccgt gagcgatgcg gtggccaatt aaggccacac
    10681 tgtcatttaa attccgtttt tccagttcaa atgcaattgc cttcaatgca ccttcgtagc
    10741 tgtggtgagc cagcggtgct ggctctcccc catttacgga taagaatgca ttttccgagt
    10801 taataccgtc ggcaatacct gacattaata cttcacagtc gctggcatcg agtacggaaa
    10861 acttaatcga agacgaacca cagttaataa ccaaaacaac cggaaattca ttcatctctt
    10921 ttctcatcct gagttacgga ttaaaacagt ttgtatacga tgttcaggat ggtcagcaga
    10981 ccaatcacgg taacaaacac gttatccaga cgaccacggt atttcgccag agacggcgct
    11041 ttacggatgg catacatcgg caacaggcac agcagggatg cgataatcgg tgcgcccatg
    11101 gcttcaataa ggtcgaggat gttcgggttg gcgtaggcaa caacccaggt ggagcccatg
    11161 atgaagatca tgctgatagt gttcagttta cccagcgaca ctttagtttt gtcgccttta
    11221 taaccgaact tcagaatcag accattcaag ccttccagcg tccccagata gtgaccgaag
    11281 aaagatttga agatagccac gagtgcgatg atggaagccg catattccag tgtaatcgcg
    11341 aacgttgttt tggtaccggt catggacgca aagtggttag ccagataaga aagcactgga
    11401 atattctgcg ctttggcttc cgccatgttg gccggagaca gcgtaaacag gcagctaaag
    11461 gcaaagaaca tcaccactgc aaccatcagc atgctggcac gagaaatgat ttgggaacat
    11521 ttacgttcgg tgaagtcgcg accgaagtct ttctcatact cttcacgttt agaaaccacg
    11581 aaggaagaga cgattggcga gaagttaaag gagaaaacca tgatggaaat ccccagccag
    11641 acagtgatca ggataccgtc atgaccggtt aacgacagcg aaccgaggtc aacctggtcg
    11701 ataactgcag agttccagta agggatcagc gacagagaaa tcagcaccag gctggcgata
    11761 aacggccata ccaggtagct catcacttta accatcagat ccttaccaaa ccagatgacg
    11821 aaagccatca gcagcaacag gaacagcgcc acaaagccgc gattcagcgg tgcaaatccg
    11881 agctggtttt cccagaacgt cataaaggta ttggtaatag taacgccata aatccacagc
    11941 agtgggcaaa tcgcgaagaa gtacaggaac gtgataacca cgccgccagt tttaccaaaa
    12001 tgctcttcca ccgtttccgt aatgttgccg gaagggttag agccagaaag acacagacgc
    12061 gccagcgccc ggtggcaata aaatgcgatg gggtatgcca ataccaacat cagaagaatc
    12121 gggatcagtc cgccaaaacc tgcgcggata gggaagaaca gcaccccggc gccgattgcc
    12181 gtaccaaaca agcctaacgt ccatgtggta tctgatttac gccaggacga ttgttttgtc
    12241 tggctggata caatgctatc tgaagtactc atatcctatc ctcaacgaat taattaagcg
    12301 tcaacgaaac cggtgatttg agagacgcga gaaagatcga tattgccgcc ggaaataata
    12361 ctgacggttt ttctgttttg aatatattgg tctaatttac cgcttaataa tgcagcacat
    12421 gccagagcgc ctgcgccttc ggtgacgact ttattgcgct gaattaaggc aatcatactg
    12481 tttctgattt cgtcttcgct gaccagcacg atgtcatcga ctaattcacg aacgatttcg
    12541 taagttaaat tacccgggcg ggagacatca caaccatccg ccagggtgcc ggtagttcgg
    12601 tgcgtggtta tttctccgga gtggaaagaa gccgccatgc cgtgaacgtt ttctgactgt
    12661 acgccaataa cacgaatggt cgggttaata gatttaattg ccaccgcaat accagcaatt
    12721 aaaccaccac caccaattgg cacaatcacg ttatcgacat catagagatc ttccataatt
    12781 tccagaccaa tcgttccctg gccagcaatc actttcggat catcgtaagg tgggataaaa
    12841 atacgacctt ccatttcgac aatttcgctc actttagcga tagtgtcgtt gaaattatca
    12901 ccatgcagaa cgacttctgc ggagtagtcg cacgttgccg ctactttgga ttttggcgca
    12961 ccttttggca tcaccacttt accgtcgata cccagcatcg cgcaggagag cgaaacccct
    13021 tgcgcatggt tgcccgcaga acaggccact acacctttac gtttttccgc atcaatcagt
    13081 gaacttaatt tattaaatgc gccacgaatt ttaaatgaac ccgtacgctg catattttca
    13141 aacttcagga atatttcacc tttgcaacgt tcactaaaat agtttgagcg aggcatgcct
    13201 gttttataaa ttcgcccagc cagtcgttgt ttcgcttcaa taatgtcatc aatagcaacc
    13261 ggcagatcgt atgtaatatg cattataaaa cctcttcgcc tgaattaaat atgtaggtaa
    13321 ccgtaaccga cacctgcaaa acagacaggt ggattattta tgtttaaata aaaacaaata
    13381 actaaccaac ttctattaat tgccttcgtc tacacccatt ataagatgaa tactctttgg
    13441 ctaattccac caaaaccgat gctgcttttt taatacgata atttttcgac cataccgcgg
    13501 catattgtgc cacaggtaat gtttcttcaa ccggaatagt aataaattga tttgaaccaa
    13561 aaggtgacgt catatcacaa ggaattacag ttaagaaatc agcattgaga acaagattat
    13621 aaattgtcac gactgagtcg gttttaacga tgttttcaat actgatgcca tttctttgta
    13681 acgtagtaag aagctcgctg tagtacccca tattggtttg tggcaacacc cactgttcgt
    13741 tcttcaacga ctccagcgtg gtggtgccgg tgcatgttcg ggacttactg gctaccaaca
    13801 caaacccgga ctcgaacagc ggctcaacat gtaaatcctg aagcttcatt tctgcactta
    13861 acgtaccaat cgcaaaatcc agtcgaccgt cgcggattgc cggtaagaac gaagacagtt
    13921 gcgcttcata catagaaact tgcgctttcg ggaacacctc tttgaacttg ttgatcatcc
    13981 ctgacataaa agtaaaacca atcaatgaag gaaaaccaaa tgagacttcc accaccgcct
    14041 cagaagacat accacttatc tcattaacca tatttttcat ttcacgggta atggattcgg
    14101 aacgggagag taacaattga ccggcaggtg ttaatgttac accggtattt ttccgcacca
    14161 ctaattccac accaaaataa tcttcaatat cgttaatgat tttactgacg gctggttgag
    14221 ttaaccctaa ttcttttgca gccgagccga tagagccact tctaatgact tcctgaaaga
    14281 ctaccaggtg ctgcgttttc ggaagaagaa tagtgctcat aacgacctac gttaattacc
    14341 tcattgacgg catgaagtgt atcaaaatga aatgaacagg atatgtgcga ccactcacaa
    14401 attaactttc aatactttcc agggtatcgt tattacaatt aaacaccttt aaatatcaac
    14461 aagttaaagc ataaaaatca gcataaaccc tgatttttgt caaaaaaaat ga
//
LOCUS       DAZFXT010000114        14501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.114, whole genome shotgun sequence.
ACCESSION   DAZFXT010000114 DAZFXT010000000
VERSION     DAZFXT010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.114"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..621
                     /locus_tag="ACHKW0_003746"
     CDS             55..621
                     /locus_tag="ACHKW0_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476506.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            672..3131
                     /locus_tag="ACHKW0_003747"
                     /pseudo
     CDS             672..3131
                     /locus_tag="ACHKW0_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            3146..3877
                     /locus_tag="ACHKW0_003748"
     CDS             3146..3877
                     /locus_tag="ACHKW0_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7476507.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3874..4935
                     /locus_tag="ACHKW0_003749"
     CDS             3874..4935
                     /locus_tag="ACHKW0_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476508.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5087..6133
                     /locus_tag="ACHKW0_003750"
     CDS             5087..6133
                     /locus_tag="ACHKW0_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HFO7476509.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6130..6921)
                     /gene="nei"
                     /locus_tag="ACHKW0_003751"
     CDS             complement(6130..6921)
                     /gene="nei"
                     /locus_tag="ACHKW0_003751"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000703 - oxidized pyrimidine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HFO7476510.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6957..7691)
                     /gene="pxpA"
                     /locus_tag="ACHKW0_003752"
     CDS             complement(6957..7691)
                     /gene="pxpA"
                     /locus_tag="ACHKW0_003752"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HFO7476511.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7681..8613)
                     /gene="pxpC"
                     /locus_tag="ACHKW0_003753"
     CDS             complement(7681..8613)
                     /gene="pxpC"
                     /locus_tag="ACHKW0_003753"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HFO7476512.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     ITDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8607..9263)
                     /gene="pxpB"
                     /locus_tag="ACHKW0_003754"
     CDS             complement(8607..9263)
                     /gene="pxpB"
                     /locus_tag="ACHKW0_003754"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HFO7476513.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9286..10029)
                     /gene="ybgI"
                     /locus_tag="ACHKW0_003755"
     CDS             complement(9286..10029)
                     /gene="ybgI"
                     /locus_tag="ACHKW0_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HFO7476514.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10300..11781
                     /gene="dtpD"
                     /locus_tag="ACHKW0_003756"
     CDS             10300..11781
                     /gene="dtpD"
                     /locus_tag="ACHKW0_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HFO7476515.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSSMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11823..13241)
                     /gene="phrB"
                     /locus_tag="ACHKW0_003757"
     CDS             complement(11823..13241)
                     /gene="phrB"
                     /locus_tag="ACHKW0_003757"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HFO7476516.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHCPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVMLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13238..13747)
                     /locus_tag="ACHKW0_003758"
     CDS             complement(13238..13747)
                     /locus_tag="ACHKW0_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HFO7476517.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13898..14501)
                     /locus_tag="ACHKW0_003759"
                     /pseudo
     CDS             complement(13898..14501)
                     /locus_tag="ACHKW0_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3508 a         3869 c         3503 g         3621 t
ORIGIN      
        1 ataagatatt cttgcaccga tatacttttt tcttttctgt taaaggactc cgttatgttt
       61 aaaggacaaa aaacattggc cgcactggcc gtatctctgc tgttcacagc acctgtttat
      121 gcggctgatg aaggatccgg tgaaattcac tttaaaggtg aagttattga agcaccgtgt
      181 gaaatacatc aggatgatat tgataaagag gttgaactcg gtcaggtgac caccagccac
      241 attaatcagt cacatcacag cgatgccgtt gctgtcgacc tgcgcttagt caactgtgat
      301 ctggaaaact ccagcaacgg ttccggtggc aagatttcta aagttgcagt cacctttgat
      361 agctcagcga aaaccaccgg cgcagatccg attctcaaca acaccagcac gggtgaagcc
      421 accggcgtcg gcgtacgttt aatgaataaa gatcaaagca atatcgtttt aggcaccgct
      481 actccagata tcgacctggc tccgacctcc agcgaacaaa cgctgaattt ctttgcctgg
      541 atggaacaaa ttgatcaggc tacacctgta acgccaggcg cagttacggc gaatgcaacc
      601 tacgtgctgg attataaata agtattactt tggcggcgct atcgccgcct ttcataaaat
      661 atttatcagg tatggacacc gtgaatattt atcgactctc ttttgtatcc tgcctggtcg
      721 tggcgatgcc ttgcgcattg gcggtcgaat tcaaccttaa tgttctcgat aaatcaatgc
      781 gcgaccgcat tgatatttca ttattaaagg aaaaaggagt cattgctccc ggtgagtatt
      841 ttgttagcgt tgcggtaaat aataaccaaa tcagtaacgg gcaaaagatt aactggcaca
      901 aaaatgacga taaaaccatt ccgtgcatca atgatttact ggtcgataaa tttggcttaa
      961 aacctgaagt ccgtcagtcg ttaccattga taaatcagtg cgtcgatttt agctcccgac
     1021 ctgaaatgct cttcaatttc gatcaagcca atcagcaact aaatatcacc attccgcaag
     1081 cctggctggc gtggcactca gaaaactgaa ccccaccctc cacatggaaa gaaggtgtcg
     1141 ccggtgtcct gatggattac aacttgtttg ccagcagcta ccgcccacag gatggcagca
     1201 gcagcactaa tcttaacgcc tatggaacag ccggaatcaa cacgggggca tggcgcttac
     1261 gcagtgatta tcagttgaat cagactgata gcgatgataa ccatgaacag tcaggcgaaa
     1321 tatcacgtac ctatcttttt cgcccgttac cgcaattagg ttcaaaatta accctcggcg
     1381 aaacggattt tagttccaat attttcgacg gtttttctta taccggcgcg gcactggcaa
     1441 gtgacgatcg aatgttgcca tgggaactac gcggctacgc cccacaaatt agcggtattg
     1501 cacagaccaa tgccacggtg acgatcagtc aatcaggccg cgtcatttac cagaaaaaag
     1561 tcccaccagg cccatttatc attgacgacc ttaatcagtc tgttcagggc acactggatg
     1621 tcaaagtgac ggaagaagat ggtcgggtga acaatttcca ggtttcggca gcatcgacgc
     1681 ccttcctgac tcgtcaggga caggttcgct ataaactggc cgcgggtcag ccacggccct
     1741 ccatgtcaca tcaaactgaa aatgaaacct tttttagcaa tgaagtttcc tgggggatgc
     1801 tgtcaaacac ctcgctgtac ggcggcctgc tgctttctgg tgatgactac cattctgccg
     1861 caatgggtat tgggcaaaat atgctgtggc ttggtgcgct gtcgtttgat gtcacgtggg
     1921 ccagtagcca ttttgatact cagcaggacg agcggggctt aagctaccgt tttaattaca
     1981 gcaaacaagt ggatgccact aacagcacga tttccctcgc cgcttatcgt ttctccgatc
     2041 gtcattttca cagctacgcc aactatctgg atcacaaata caacgacagc gatgcgcagg
     2101 acgaaaaaca gacgatcagc ttatctgtgg gccaaccgat taccccacta aacctcaatc
     2161 tttacgccaa cctgctacat caaacctggt ggaatgcaga cgcctccacg accgccaaca
     2221 tcacggcagg ttttaatgtt gatattggtg actggagaga tatctctatt tcgacgtcat
     2281 tcaatacaac ccattacgaa gataaagatc gcgacaacca gatttacctg tcgatttcgc
     2341 tccccttcgg taacggtggt cgggttggct atgacatgca aaacagtagc cacagcacca
     2401 cacaccgcat gtcgtggaac gatacgctgg atgaacgtaa tagctggggc atgtctgccg
     2461 gactgcaatc cgaccgtcct gacaatggag cccaggtgag cggtaactat cagcacctga
     2521 gttcagcggg tgagtgggat atttctggta cctatgccgc caatgattac agttccgtca
     2581 gcagcagctg gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt catcgccgca
     2641 gctccaccaa tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat attccggttc
     2701 agggcaatct cgactacacc aaccattttg gcattgcggt ggtgccgttg atttccagtt
     2761 accagccttc caccgtggcg gtgaacatga atgacttacc cgacggcgta acagttgcag
     2821 aaaacgttat caaagaaacg tggattgaag gcgcgatagg ttacaaatca ctggcttccc
     2881 gttccggtaa agacgttaac gtcatcattc gcaacgccag cggtcagttt cctcccctcg
     2941 gagcggatat ccgccaggat gacagcggca ttagcgtggg gatggttggc gaggaaggac
     3001 atgcctggtt aagcggagtc gctgaaaatc aaaagtttac cgtggtctgg ggtgatagcc
     3061 agcattgctc gctccatctt cctgaacata tggaagacac cgcaaatcgc ctgattttac
     3121 cttgtcatta attaaggaaa tgaaaatgac atttatgaaa ggactacccc ttttgttgtt
     3181 ggttgccagt ctgtgcagcc acgctgcact acaacccgat cgcactcgta ttgtgtttaa
     3241 cgctaatgat aaagctacca gtctgcgggt ggataatcgt agcgataaac tcccctacct
     3301 cgcttattcc tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc tggtcgccct
     3361 gccgccgata caacgtctgg aaccgaaagc aacaacgcag gtacggatag taaagcaagc
     3421 ctcgaccaca aaattaccgg gcgatcgcga aacgctattt ttctacaata tgcgtgaaat
     3481 tcctcccgcc ccggaaaaaa acagcgacca tgccgttctg caagtcgcca ttcagagtcg
     3541 tatcaaggtg ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa aggtcgaact
     3601 gcagttacag gtcagccaac agggcaacca actgacactg aaaaatccta ccgcgtatta
     3661 tctgaccatt gcctacctgg gacgaaatga aaaaggcgtt ctccccggct tcaaaaccgt
     3721 gatggttgcg ccttttagta cggtcaacac aaacaccgga aattatagcg gcagccagtt
     3781 ttatctcggt tatatggatg attacggcgc gttacgcatg acgacgctca actgcagcgg
     3841 acaatgccat ttacaagccg tggaggcgaa gaaatgagtg ctggcaaggg agtgttgctc
     3901 gtcatctgtc tgttatttct gccgttgaag tctgcgcttg ccctgaactg ctactttggt
     3961 tcatccggtg gctcggtaga aaaatcagaa gcgattcaac cttttgctgt accaggcaat
     4021 gccaaactcg gcgataagat ttgggaatct gacgatatta aaattcccgt ctattgtgac
     4081 aacaatacca acggcaattt tgaaagtgag catgtttatg cctgggtaaa cccctacccg
     4141 ggggtgcagg atcgctatta tcaactcggc gtaacttaca atggcgttga ttatgacgcc
     4201 aaccagggaa aaagccgcat cgacactaac cagtgtattg acagtaagaa cattgacatt
     4261 tatacccctg agcagattat cgcgatggga tggcagaaca aaatttgctc gggtgatccc
     4321 gctaatattc acatgtcacg tacctttctc gctcgcatgc ggttatacgt caaaatacga
     4381 gaaatgccgc cgcatgatta tcaaagtacg cttagcgact atatcgtcgt gcaatttgac
     4441 ggtgccggta gcgttaacga agatcctact gcccaaaacc tgaaatatca tattaccggt
     4501 ctggaaaaca ttcgcgtgct ggattgcagc gtcaattttt ccatttcacc ggaaacacag
     4561 gtgattgatt ttggtaaatt taatttgctg gatattcgtc gccacacaat gtcgaaaaca
     4621 ttcagtatta agacgacaaa atcacaaaat gaccaatgca ccgacggatt taaagtcagt
     4681 tcctcctttt ataccgaaga aacgttggtt gaagaagata aagcattgtt gattggtaat
     4741 ggtctgaagc tacgtttgct ggatgaaaac gcctcgcctt ataccttcaa taaatatgct
     4801 gaatacgccg atttcaccag cgacatgttg gtctacgaaa aaacctatac agctgaactt
     4861 tcgtccattg caggtacccc catcgaggct gggcccttcg atacagtggt gctttttaaa
     4921 attaactata actgatttgc catgatgccc tttgcagccg gaaagggcat ctctttcaga
     4981 aaattgctga ttttcccctt ttccttgatt gctgttattt tttcctggcc cggctttctt
     5041 tatgctgtat cgatacacca aagagaaaat aatgagggag cgaaggatgc cagttttgca
     5101 gtggggaatg ttatgtgtgc tatcacttct cctttctatt ggtttcctcg cggtacatct
     5161 cccggcggcg ctattacttg ggccgatgat cgccgggatc atcttcagta tgcgcggaat
     5221 tactctgcaa cttccccgct ccgcttttct cgccgcgcag gccattcttg gctgcatgat
     5281 tgcgcaaaac ctcactggtt cgattctcac cacgctggcc gccaactggc cgatcgtgct
     5341 tgctatttta ctggtgacgc tgctttccag cgccatcgtg ggttggttat tggtgcgcta
     5401 tagctcactg cctggaaata ccggtgcctg gggttcttct ccaggcggcg cagcggcaat
     5461 ggtcgctatg gcgcaagatt acggcgcaga tattcgcctg gtggcgttta tgcaatatct
     5521 gcgagtgctg ttcgtcgcgg gggcagctgt tctggtcacc cgcatgatgc tgggtgataa
     5581 cgctgaagcg gttaaccagc aaatcgtctg gttcccgcca gtgagcatta atctcctgct
     5641 tacgattttg ctcgcagtcg ttgccggtac ggcgggatgc atgctgcgtc ttccttccgg
     5701 cacgatgctc atcccgatgc tggcgggcgc agtgctccag tctggtcagc tcatcaccat
     5761 cgaactcccg gaatggttgc tggcgatggc gtatatggca attggttggc ggattggtct
     5821 tggtttcgat aaacaaatct tactgcgggc attgcgccca ctgccacaaa tcctgctgtc
     5881 gatttttgct ctgctggcta tttgtgcggg tatggcgtgg gggctgaccc ggtttatgca
     5941 cattgatttt atgactgcct acctcgccac cagtcccggc gggcttgata ccgtggcggt
     6001 catcgccgca gggagcaatg ccgatatggc gcttattatg gcgatgcaaa ccctgcgcct
     6061 gttcagtatt ttgctgacgg ggccagccat tgcgcggttt atttcaacct atgcgccgaa
     6121 gcggtcggcc tagtgctggc agcctggaca ccagtaaaac gggcgcgagg acaacgtggt
     6181 tttctcaatg atgccgccac agcgttcgca cagttcgcca tcacgatgaa aaaccttaaa
     6241 gcgaaacagc gccccatgat gcttattctc atccacctgc ccccgcgtag cgtaagaaaa
     6301 ccgaggaatc tccagtaacg cgtgtgcgag tgcatccagt tgcgccgcat tgagatcttt
     6361 cgctttatga tttccagtca acccaacctg ccagaggatc tccacccgca aataattgcc
     6421 aagcccagcc agaaacgcct gatcgagcag taatcctgca aactgacggt tacgaaagcg
     6481 cggtgacaat aaccgttcct ttaccacctc cggcgtcaga ttcggatcca gcacatcggg
     6541 accaacgcgt tgtaaaaacg gatgcgtggt cagttgttcc ggcctcaaca tctcaatatc
     6601 cgaggcgcta taaagcagaa tggttttgtc agccgtttgc agttttaccc gcaatactcg
     6661 cgtggtctgc ggctcttcgc cggtatcaac cacgcgccag acgccgtaaa gctgattatg
     6721 gctgtagagc gttaagtcgt tggaaaaatg agttaacaac gccttaccac gcgtttccac
     6781 atgggtaacg tgttgaccga taagttgtga ttgataagtt tttaactgcg ggaaggcaaa
     6841 ccagacatca gttagtggtt tgcctttgat cgccgcctcc aggttatccg ctgcacggcg
     6901 gatctccggg ccttcaggca tggtgatatc cttttttcat tctttattaa tcggggttat
     6961 gctgcgacaa caatctcctt ttcggcaaat gtagagcgta gtcggcgggc gaacgccagt
     7021 gcgtgctcgc cgtcgccatg caggcagact gtttgcgccg tgaccgttgc ccattcgccg
     7081 gtgatacttt tgactctgcc gtgttgcacc atctccagcg tttgcgccaa cgcctgttct
     7141 tcgttttcaa tcaacgcgcc tcgctgactt cgcggcacca gcgagccgtc agcctgataa
     7201 ccgcgatcgg caaacacttc ctcgcgcgtt gtcaggccat attgcttgcc tgcacgaatc
     7261 agctcgcttc ctgccagccc aacgagaatc aatgccggat cgcaagcgta taccgctctg
     7321 gcgatggcgt ctgccagttg tgcttctttc gccgcctggt tgtacaacat gccgtgcggt
     7381 ttgacatgac gcattacgcc gccttgcgca cgggcaatcg ttgccagcgc gccaatttga
     7441 tacagcgtct gggcgtaaac ggtttctggc ggcagctgca tggcgctgcg accaaaattt
     7501 tccctgtcgg gaaaactcgg gtgagcgcca atcgcgacac cattttttat tgcttcacgc
     7561 acgcaagcct gcatgatttg cgcatcgcct gcatgaaatc cacaggcaat attggcagag
     7621 gaaaccagcg ttaatagctc tgcgtcgctg gcgcagcctt cgcccagatc ggcgttcagg
     7681 tcaattttca ttgtgcagcc gccacgctaa ttgttcgaaa taacgttgct gatcttgccg
     7741 cgctttcagt gcctcttcca gtgaacactg gacaaaatga atcggctgac cgagcggaat
     7801 ttgcgccaga tggtacatat cagcctcaat gatacaggca atacgcgggt aaccaccggt
     7861 ggtctgtgcg tcgttcatca acacaatcgg ctgcccgtta tgcggcacct gcaccacgcc
     7921 cggtaacaaa ccgtgagata acagttcgcg atcggtgatg cgttttaaaa tttgcccctg
     7981 taagcgatag cccattcggt tactttgcga gctaagctgc cagggcgaac gccagaatgc
     8041 atcctgcgag gcgcgatcga actcatgata ttccggcccc ggcaaggcgc gaatgcggtt
     8101 gccccacagc agctgtttaa cgccctgcgc ttccataaaa tcacgcttcg atttgccaat
     8161 cgggagtcgg tcaccatcct tcagtaaacg gccttccagc ccgccaatcc ccactttgag
     8221 atcggtgctg catgacccca ttaccggcgg aacatcaata ccacccgcga ccgccagata
     8281 actgcgcatc ccgtgctgcg ggcgtttaag cgttaaacgc tggcctgctt tcatcggcaa
     8341 tcgccagccg gtccagacgg cattatcatc cagccgcgct tcgcaaccgg cacccgtcag
     8401 agcaaaccac ccatcagttt cgaactcaac cgttaactga ccgagcgtga tctccagcgc
     8461 gggggcattg gcgtcattac ccaccagcag gttagcaatg cgtaacgcag gcatatccag
     8521 tgcgccgcag tggctgatac ccgactggcg aaaaccgtga cgaccgccat cttgcacagt
     8581 ggtatacatg cccgcacgaa taatcttcag catactccct ccttttgcgg tacaaagcgc
     8641 acgctgtctc ccggacgtaa taagatgggt tcgtcacgcg ccggatcaaa caggctgagt
     8701 gaggtatgac caatcaactg ccacccaccc ggcgttgcca gcggataaac accagtctgc
     8761 ggcccgccga tcccgacaga acctgccgga acgagcaagc gcggttcagc gcgccgtggc
     8821 gtgtgtagtt gttccggcaa actcccgaga tacgggaagc ccggttgaaa acctaaaaac
     8881 cagaccacgt attccacgga ggagtgcaat tcaacaacct gtttttcgct caacccgcaa
     8941 tgcgccgcga ccaccgccaa atccggtccg cctgcaccac cgtaaaccac cggaatttca
     9001 ataaagcgag actcaggttc cagggcctcg ctctcctccc accagcgttg caaacgctct
     9061 atggcatcca gcgccagcga ctcaggatta cgcaaaatca ccgtgatatt gttcatgccg
     9121 ggaatggctt caaccacatt cggcatatcc accagacgct gcgccagtcg ccagatccgt
     9181 ttctggctag ccagcgtcac cggcggttcc agttccagca ctaccgccgt ttcacctatc
     9241 agataacaac gcgctcgttg cactttcgat ccctctgatt attcgttatg caggattagg
     9301 tatatcaata aaggtcacat caagatcggt attttcattc agccactcac tcaatgcgcg
     9361 aataccaccg cgttcagtgg catggtgacc tgcagcataa aaatgcaatc cctgctcgcg
     9421 ggctgaatga atggtctgtt cagaaacttc gccagtaata aaagcatcca cgccaaaacg
     9481 cgcggcgcta tcgataaaac tttgcccgcc gcccgtgcac caggcgacgc gctggaccac
     9541 ctcaggtccg gtatcgccac accataacgg cttacgtccc agacgcgctt caatccagga
     9601 ggctagttcc agtcccggca caggcatggt cagctctccc cacggcacca acggctcaat
     9661 ttcgcccatg accgtgatcc ccagtaacgc cgccagctgt gcgttattgc ccagctcagg
     9721 atgtgcgtca agcggcaaat gccagccata caggttgata tcattcgcca gcaacgtttt
     9781 taaacggtta cgcttcattc cgcgaatgac cggagactct cctttccaga agtagccatg
     9841 atgcacaatg accgcatccg cgcccagacg cactgcctca tcgagcaaag cctggctggc
     9901 ggtgacaccg gtaacaattt tttgcaccgt ctctttgcct tccacctgca aaccattcgg
     9961 cgcgtaatca ctaatcgccg cgctgttcag tttttcgtta atcagttgtt ccagttcggt
    10021 gtttttcatc ttcgcgcctt ccgtattctg atagctacac cgttaacata gcgttacctc
    10081 gcctgttcgt cgatagccaa tcttttgaca tattaagaaa caaacccgac gccaaaagac
    10141 gcaaattgtt tatatcatca cgcttcagct aatgtatatt tatgcaatca gacagcatat
    10201 tgcataaata atcagatctg tatcaaagtt ttaactttaa ggaaacacaa gcacgccgcg
    10261 caactattac gtgacgttgc cgtcaattgt ggaattatta tgaataaaca cgcatcacaa
    10321 ccgcgcgcta tttactatgt cgttgcgctg caaatctggg aatattttag cttttacggc
    10381 atgcgtgccc tgctgattct ctatctcacc aatcaactaa aatacaacga tactcacgcc
    10441 tacgagttat ttagcgccta ctgttcgctg gtgtatgtca cgccaatcct cggtggcttt
    10501 ttggcggata aagttctcgg caatcgcatg gcggtgatgc tgggggcgtt gttgatggcg
    10561 atcggtcatg tggtgctggg tgccagtgag atccatccgt cattcctcta tctgtccctg
    10621 gcgattatcg tttgtggcta tggtctgttt aaatccaatg tcagttgcct gctcggcgag
    10681 ctgtatgagc caaccgatcc gcgtcgtgat ggcggtttct cgctgatgta tgcagcgggt
    10741 aacgtggggt ctattatcgc acctatcgcc tgtggttacg cccaggaaga gtacagctgg
    10801 gcgatgggct ttggcctggc ggcggtgggt atgatcgcgg gtctggtcat tttcttatgt
    10861 ggcaatcgtc atttcacaca tacccgcggc gttaataaaa aagtactgcg tgcgacaaac
    10921 tttctcctgc ctaactgggg atggctgctg gttctgctgg tggcaacgcc tgcgctgatt
    10981 accgtattgt tctggaaaga gtggtcggta tacgccttaa ttgtcgcgac tattattggc
    11041 ctgggtgtac tggcaaaaat ttatcgcaaa gcagaaaacc agaaacagcg taaggagctg
    11101 gggctgattg tgacgctcac cttcttcagt atgttgttct gggccttcgc acaacagggc
    11161 ggcagctcga ttagccttta tatcgaccgc ttcgttaacc gcgatatgtt tggttatacc
    11221 gttccgactg ctatgttcca gtcgattaat gccttcgcag ttatgctgtg cggtgtgttc
    11281 ctggcgtggg tggttaaaga aagcgtcgca ggtaatcgta ccgtgcgcat ctggggaaaa
    11341 tttgctcttg gtctcggcct gatgagcgcc gggttctgca ttctgacctt aagcgcccgc
    11401 tggtcctcaa tgtatggtca ctcttctctg ccactgatgg tattaggcct ggcggtgatg
    11461 ggctttgcgg aactgtttat cgacccggtt gccatgtcgc aaattacgcg cattgaaatc
    11521 cccggtgtga ccggcgtatt aaccggcatt tatatgctgc tttccggcgc gattgcgaac
    11581 tatctcgcgg gcgtgattgc cgatcagaca tcgcaggctt cgtttgatgc ttccggggcg
    11641 atcaactact ccatcaatgc atatattgaa gtatttgatc aaattacctg gggcgcactg
    11701 gcgtgtgtag gactggtact gatgatttgg ctgtatcagg cgctgaaatt cagaaaccgc
    11761 gcgctggcgc tggaatctta ataggccagc accatacctg ataaaacgct cgctgcgttt
    11821 tatcaggctc ctttcctcgc ttcttcgtac gctgccagcg ttcgcagcct tgcctctttg
    11881 tgatcaacta tcggttgcgg ataatccagc atcacacctg ctttctgcgc ccacttccac
    11941 ggctcatgca ccgctttccc tggtacatcg cgcagctccg gtaaccatcg gcggataaac
    12001 tcgccctcac ggtcaaattt ctcgccctga gttatcggat tgaaaatacg aaaatacggc
    12061 gcagcatcag tacccgtaga agccgcccac tgccagccac cgttattagc tgccaaatca
    12121 ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caacaaatct
    12181 ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc agtactgtta
    12241 agctgacgca tagcggcatc aacaatcggg tatcccgttt tgcctttctg ccaggcctgt
    12301 aaatgtgcgg gattgctttg ccactgcaca cgatccgtcc aggcaataaa cggacaatgt
    12361 ttacacaacg aggggtaata cgtcatcaga tgacggtaga actcgcgcca gatcagctca
    12421 ctaagccaga cactaccggc cccaccgtcc agcgcctgcg gctgttccgc caacaagcga
    12481 tgcaaacact ggcgaggcga taacccgccc gttgccaggc tggcggacaa acgactggtg
    12541 ccttccactg ccggaaaatc tcgttgttgc tcatattctc cggcaccgtt ctggcaaaac
    12601 tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt atcgaaagac
    12661 tgacgaggat aattcagcgt aatggatggc gcgggctcta tcgatccgct actacgaact
    12721 tttggcgcag cgacacactc cggcatccct tcccgcagcc gtttcagcca ggcattctta
    12781 aaaggcgtaa agactttgta catctcgtga ttaccggtca tcaccgcgcc aggcggcagg
    12841 atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc aacttccaca
    12901 tcccgcgccc gctcattcac ttcatactga tagttataaa acaggtgggt aacgctgttt
    12961 tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac
    13021 aataaaggaa tacctttttc cgcaagcgct atttgtagcc cattcagttg agcattgata
    13081 agttcagcct gacgcggcga catgttatgc gtcgcccact ggcgtggtgt agcgatatat
    13141 aacgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc
    13201 agacgtaaat cctggcgaaa ccagaccaga tgggtaatca taaaactcct gaatggcgca
    13261 agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat
    13321 aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc
    13381 gataactgtc gataagcgcc gccaactgct ggcgttctgt ggaatcaata tgcgggcgaa
    13441 aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg ggatgagaaa
    13501 gcaaaacaat tacgcgttgg tagtactggt tgtaaaaatc atccagattt tgccactggt
    13561 gaatatcagc cacgaacggg ccaagtttgc ggtactccgg ctgagaatgc gccagaaaga
    13621 cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca
    13681 gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt gctgtcatca aatcgttgct
    13741 gattcattac tgctcctgaa cggtttacag gagtaagtgt agacgttgag aaatgatatt
    13801 tgtgaagtta atcctgttgt agcaggacaa atttacgaaa gcctgctccc cgcaaggact
    13861 gacgtcaggt agtttctaac catagctgcc ctttctattc aaacgtgaaa tcaataagct
    13921 catcagggac atcgcaaaca atatgaagac gggtttcttc tctgccgtga ctcttttcac
    13981 taattgcgta actgtcatgc ttgggattat ttaattcttt cagcggaaat ttttcctcga
    14041 aggctttatt tagccgcccc tggtttcctt ttacagcgaa taaataatca cctccctgtt
    14101 tttgtatctt ctctgcaata tctttctggc aacccatcgc atcggttgtg atgatttttc
    14161 ctttaatatc cagcatgtta agaagttcag ggatagctgt gatctcattg gatttcttat
    14221 ccgtcttgat ctgcccgatg accagactgt gcattgttga gaacgcacta atgacatgaa
    14281 tcgctcccct gcggcgactc ttgtcataag agtgccggag cgtttttcca tcaattgcaa
    14341 tgacgtcttt atcatttgat gaatggcaat cacgcatcca gttaataaag cactcgtgaa
    14401 attttgcagg actgatacag gatacaactc tggcaatagt atcgtgaaca ggaataccat
    14461 tttcaaaatc accatattgc ttcaagaaat cgagatgtgt t
//
LOCUS       DAZFXT010000115        14432 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.115, whole genome shotgun sequence.
ACCESSION   DAZFXT010000115 DAZFXT010000000
VERSION     DAZFXT010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14432
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.115"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            415..1986
                     /gene="garD"
                     /locus_tag="ACHKW0_003760"
     CDS             415..1986
                     /gene="garD"
                     /locus_tag="ACHKW0_003760"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008867 - galactarate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0046392 - galactarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HFO7476518.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGMAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            2135..2470
                     /gene="prlF"
                     /locus_tag="ACHKW0_003761"
     CDS             2135..2470
                     /gene="prlF"
                     /locus_tag="ACHKW0_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HFO7476519.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2470..2934
                     /gene="yhaV"
                     /locus_tag="ACHKW0_003762"
     CDS             2470..2934
                     /gene="yhaV"
                     /locus_tag="ACHKW0_003762"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HFO7476520.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2989..3798)
                     /gene="agaR"
                     /locus_tag="ACHKW0_003763"
     CDS             complement(2989..3798)
                     /gene="agaR"
                     /locus_tag="ACHKW0_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HFO7476521.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            4047..5327
                     /gene="kbaZ"
                     /locus_tag="ACHKW0_003764"
     CDS             4047..5327
                     /gene="kbaZ"
                     /locus_tag="ACHKW0_003764"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HFO7476522.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYTSANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARAMLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            5350..5823
                     /gene="agaV"
                     /locus_tag="ACHKW0_003765"
     CDS             5350..5823
                     /gene="agaV"
                     /locus_tag="ACHKW0_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HFO7476523.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5834..6613
                     /gene="agaW"
                     /locus_tag="ACHKW0_003766"
     CDS             5834..6613
                     /gene="agaW"
                     /locus_tag="ACHKW0_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HFO7476524.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6603..7481
                     /gene="agaE"
                     /locus_tag="ACHKW0_003767"
     CDS             6603..7481
                     /gene="agaE"
                     /locus_tag="ACHKW0_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HFO7476525.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7499..7933
                     /gene="agaF"
                     /locus_tag="ACHKW0_003768"
     CDS             7499..7933
                     /gene="agaF"
                     /locus_tag="ACHKW0_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine/N-acetylgalactosamine
                     transporter subunit IIA"
                     /protein_id="HFO7476526.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7930..9063
                     /gene="nagA"
                     /locus_tag="ACHKW0_003769"
     CDS             7930..9063
                     /gene="nagA"
                     /locus_tag="ACHKW0_003769"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HFO7476527.1"
                     /translation="MTPVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNTIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAQPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGNTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASVVALDSGLHVQQIWIQGQLASF"
     gene            9414..10568
                     /locus_tag="ACHKW0_003770"
     CDS             9414..10568
                     /locus_tag="ACHKW0_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HFO7476528.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10581..11441
                     /gene="kbaY"
                     /locus_tag="ACHKW0_003771"
     CDS             10581..11441
                     /gene="kbaY"
                     /locus_tag="ACHKW0_003771"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HFO7476529.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11608..12084
                     /gene="agaB"
                     /locus_tag="ACHKW0_003772"
     CDS             11608..12084
                     /gene="agaB"
                     /locus_tag="ACHKW0_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIB"
                     /protein_id="HFO7476530.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            12123..12926
                     /gene="agaC"
                     /locus_tag="ACHKW0_003773"
     CDS             12123..12926
                     /gene="agaC"
                     /locus_tag="ACHKW0_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IIC"
                     /protein_id="HFO7476531.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAAIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12916..13707
                     /gene="agaD"
                     /locus_tag="ACHKW0_003774"
     CDS             12916..13707
                     /gene="agaD"
                     /locus_tag="ACHKW0_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactosamine transporter subunit IID"
                     /protein_id="HFO7476532.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13708..>14432
                     /locus_tag="ACHKW0_003775"
     CDS             13708..>14432
                     /locus_tag="ACHKW0_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HFO7476533.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDVSQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQQLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWL"
BASE COUNT         3493 a         3632 c         3759 g         3548 t
ORIGIN      
        1 gcacgtcttt ctttttttcg tcaacggtgt ccagaatcat tttatttacc tcgggtactt
       61 atgctgattt ttattattat ggggaaggtg ttatttatga gtttcattta tgccgtaacg
      121 gcaatgaact cgggaattag tataagcagc gcgagaataa taatcattgt gcaaatgcta
      181 atttaattaa tgctatttaa atattatttt gagcatatgc acataaggtt gcgcgctaaa
      241 gcacagattt gcgctttacc ttgccgggcg gcactgcaat ccctgaaatg attgacattg
      301 atcacatttc tgcgtttaaa ctcctgacat tcttatttca cccaatgaag tcatttattt
      361 ttaaatgaga ccaggtcctc attttaataa cccctggctg gagaatatag tacaatggcc
      421 aacatcgaaa tcagacaaga aacgccaact gcgttttata taaaagttca cgacacagat
      481 aatgtggcaa ttattgttaa tgataatggc ctgaaagcag gaacgcgttt tccggatgga
      541 ctggaattaa ttgaacatat tccccagggg cataaagtcg cattgctgga cattccggct
      601 aatggtgaaa ttattcgtta tggtgaagtg attggttacg ccgtgcgtgc aatcccacgc
      661 ggaagctgga tcgacgaatc aatggttgta ctaccggaag cgccgccgtt acacacgctg
      721 ccactggcaa ccaaagtccc ggccccctta ccgccgctgg aaggatacac ctttgagggc
      781 tatcgcaatg ccgatggcag cgtgggcacc aaaaacctgc tcggcatcac caccagcgtc
      841 cactgtgtgg caggcgtggt ggactatgta gtaaaaatca ttgaacgcga tctgctaccg
      901 aaatacccga acgtcgatgg cgtggtgggg ctgaatcatt tgtacggctg tggcatggcg
      961 attaacgcac cggcggcagt tgtgcctatt cgtaccatcc acaatatttc gctgaaccct
     1021 aactttggcg gcgaagtaat ggtgattggt ctgggttgtg aaaagttgca gcctgagcgc
     1081 ctgctggtcg gcacggatga tgtgcaagct attccagtag aaagcgccag cattgtcagt
     1141 ttgcaggatg aaaagcatgt cggttttcag tccatggtcg aggatatttt gcaggtcgcc
     1201 gaacgccatc tacaaaaact gaatcaacgt cagcgagaaa cctgtccggc ttcagaactg
     1261 gttgtcggca tgcagtgcgg tggcagcgat gccttttctg gcgtaacggc aaacccggcg
     1321 gttggctatg cgtctgatct actggtgcgc tgcggcgcaa cggtgatgtt ctcagaagtc
     1381 acggaagtgc gtgacgcgat ccatctgctg acaccacgcg cagtgaacga agaggtcggc
     1441 aaacggctgc tggaggagat ggagtggtac gataactatc tcaatatggg aaaaaccgac
     1501 cgcagcgcca acccttcgcc gggcaacaag aaaggcggtc tggcaaacgt ggtagagaag
     1561 gcactcggct ccattgctaa atcgggtaaa agcgcaattg ttgaagtgct gtcgcccggt
     1621 caacgcccga ctaaacgcgg attaatttac gccgcgacgc cagccagcga ttttgtctgt
     1681 ggcacgcaac aggtggcttc gggtatcacc gtgcaagtgt ttacgaccgg tcgtggtacg
     1741 ccgtacggcc tgatggcggt acccgtcatt aaaatggcga cccgcaccga gctggcgaac
     1801 cgctggtttg atttaatgga tattaacgcg ggcactatcg ccaccggcga agaaaccatt
     1861 gaagaggtgg gctggaagtt gttccacttt attctcgacg tcgccagcgg gaagaagaaa
     1921 accttctcgg atcaatgggg gctgcataac cagctggcag tgtttaaccc ggcaccggtg
     1981 acctgatttc ttttggaacg tccctcgcaa aacatggcct tagtgccatg tttttattgt
     2041 ttaaagcccc cacgtccatt aataatgcat ttgcattacc ttagttcaag cttataattt
     2101 gagcagaaaa caggctgtaa aaggacagtg aatcatgccc gctaatgctc gctctcacgc
     2161 tgtactgacc actgaatcaa aggtcacgat acgcggacaa acaactatcc ccgcgccagt
     2221 gcgtgaggcc ttaaaactga agccaggcca ggacagcatt cattacgaaa ttctgcctgg
     2281 tgggcaagtc tttatgtgcc gactgggaga tgaacaggag gatcatacta tgaatgcatt
     2341 tttgcgtttt ctggatgcag atatccagaa caacccgcaa aaaactcgtc cattcaacat
     2401 tcaacaagga aagaaacttg tcgctggcat ggacgtcaac attgatgatg agattggcga
     2461 cgacgaataa tggattttcc acaaagggtt aatggttggg cgctatatgc tcatccctgt
     2521 tttcaggaaa cctacgacgc tttagttgcc gaagtcgaga cattaaaggg aaaagatcct
     2581 gaaaattatc agagaaaagc cgccacaaag ttattggcgg tagtccataa agtgattgag
     2641 gagcatatca cggtcaatcc atcatcaccg gcattccgtc atggcaagtc gttaggctct
     2701 gggaaaaata aagactggtc acgggtaaaa tttggtgctg gtcgttatcg tctcttcttt
     2761 cgttatagtg aaaaagagaa agtcatcatt ctgggatgga tgaacgatga aaacactctg
     2821 cgcacctacg gtaaaaaaac agatgcctat accgtattca gcaaaatgtt aaaaagagga
     2881 catcctcctg ccgactggga aagcctcacc caagagacag aagaaaacca ttgatggtgt
     2941 tcacaatgcc cgccgaacgt attcgtgttg accagcgggc accctttctc actccccgac
     3001 cagaatcact tcaaccccag cctttcgcag tccttccaga ctatccgcag gaatgccttc
     3061 atcaacaatg atcatgtcga tacgttgagt atcaatgatc ttatgtaaac tggaacgatt
     3121 gaatttactg gaatcggtga ccacgatgat ccgttccgca acttcgcaca tccgacggtt
     3181 taaacgggct tcatcttcat tatgtgtgct gacgccgcgc tccagatcga tcgcatctac
     3241 accaagaaac agcatatcga agtggtaatt ttgcagcgat tgctcagcct gatcgccgta
     3301 aaaagattgc gactgacggc gcaaatgccc gccggtcatc agcagctcaa cgccttccgc
     3361 ttccagcaac gcattagcca cgttcatacc gttggtcatc gcaattacgt cagtgtgctt
     3421 acgcatcaga cgagcaatct caaaagtggt agtcccggaa tcgaggatca cccgatgacc
     3481 aggctgaatc aactcaacgg cagctttcgc aacgctgcgt ttcatcgcgg tgttcagcgc
     3541 gcttttatct tccactgatg gctcgactga cggcgtcgtg ctatcgcaga tcaacgcgcc
     3601 accataggca cgcacagcga tcccctgctt ttccagaaac gccagatcat tgcggatcgt
     3661 cacagtagat acgccataca atgccgacag atcgttaacc tgcacactcc cttgctgtcg
     3721 cagacgctga atgatctgtt ctcgtcgctc gctggtgcca gtcactcgct tctcacctga
     3781 agcgtcggta ttactcatag taagtccttt cgtaaaactt tcgtttcatt tcgttttgcc
     3841 tattaacgcc tttctattaa gcaaatgcaa gcccaccttg cccattgacg caagctactc
     3901 tcgtttcagt gactttcatt atgtttcttt tgtgaatcag atcagaaaac tattatcttt
     3961 cgttttattt ttatctcacc atgacgcagt atcaactgaa acaaaacgaa agattaatat
     4021 cgcagtaatc tgaactggag aggaaagtga aacatctgac agaaatggtg agacagcaca
     4081 aagcgggcaa aacaaatgga atttatgccg tttgttccgc acatccgctg gtgctggagg
     4141 ctgcaatccg ctacaccagt gcaaaccaaa cgccgttact gattgaagca acctccaatc
     4201 aggtagacca gttcggcggt tataccggaa tgacgcccgc cgattttcgc ggctttgttt
     4261 gtcagctcgc cgactcgttg aatttcccgc aggatgcgtt gattctgggt ggtgaccatc
     4321 tggggccaaa ccgctggcaa aacctgccgg ccgctcaggc aatggccaat gccgatgatt
     4381 tgattaaaag ctacgttgcg gcaggattca aaaaaatcca ccttgattgc agcatgtcct
     4441 gtcaggacga tccgattccc ttaactgatg acatcgtggc tgaacgcgcc gcccgtctgg
     4501 cgaaagtggc ggaagaaacc tgtcttgaac actttggcga agccgatctg gagtatgtca
     4561 ttggtaccga agtgccggta cctggcggcg cgcatgaaac cttaagcgag ctggcggtca
     4621 ccacgccgga tgccgcccgc gccatgctgg aagcccatcg tcacgccttt gaaaagcaag
     4681 gtttgaatgc catctggcca cgcatcattg ccctggtggt tcaacccggc gtcgaattcg
     4741 atcacaccaa cgttattgat tatcagcccg ccaaagcgag cgccttaagc cagatggtcg
     4801 aaaactacga aacgctgatt ttcgaagcgc actctaccga ttatcaaacg ccgcaatcgc
     4861 tgcgccagct ggtgattgac cactttgcca ttctgaaagt tggcccagcg ctgaccttcg
     4921 ccctgcgtga agctctgttc tctctggcgg cgattgaaga agaactggtg ccagcgaaag
     4981 cctgttctgg tctgcgtcag gtgctggaag acgtgatgct cgaccgcccg gaatactggc
     5041 aaagccacta ccacggtgac ggcaacgcgc gtcgtctggc gcgtggttat agctactcgg
     5101 atcgcgtgcg ctattactgg ccggacagcc agattgatga cgctttcgct catctggtac
     5161 gtaatctggc ggattcacca attccgctgc cgctgatcag ccagtatctg ccgctgcagt
     5221 acgtgaaagt tcgctccggc gagctgcagc caacgccacg ggaactcatt atcaaccata
     5281 ttcaggacat cctggcgcag taccacacag cctgtgaagg ccaataagca aaacaaagag
     5341 gaacacgcta tgccaaatat tgttttaagc cggattgatg aacgcttgat tcacggtcag
     5401 gtcggcgttc aatgggtcgg atttgcgggg gcaaatctgg tgctggtagc caacgatgag
     5461 gttgccgaag atccggtaca acaaaacctg atggaaatgg tactggcaga agggatcgcc
     5521 gtacgtttct ggacgctgca aaaagttatc gacaacattc atcgcgccgc cgatcgacag
     5581 aaaatcctgc tggtttgtaa aacacccgcc gatttcctga cgctggtgaa aggtggcgtt
     5641 ccggtgaatc gcattaacgt tggcaatatg cactacgcca atggcaaaca acaaatcgcc
     5701 aaaacggttt ctgtggatgc gggcgatatc gcagcattta acgacctgaa agccgctggg
     5761 gtggaatgct tcgttcaggg cgtcccgaca gagcctgctg tggacctctt taaattactt
     5821 tgagggattc atcatggaaa tcagcctgtt gcaggcattt gcgttgggca ttatcgcctt
     5881 tatcgctggc ctggatatgt ttaacggcct aacacatatg caccgcccgg tggtcctcgg
     5941 cccgttggtc gggctggtac ttggcgatct gcataccgga attttaaccg gcggtacgct
     6001 ggaactggtg tggatggggc tggccccgct ggcgggcgca cagccgccta acgtgattat
     6061 cggtactatc gtcggcacgg cgtttgccat tactactggc gtgaaacccg atgtcgcggt
     6121 cggtgtcgcc gtacctttcg ctgtcgcagt acagatgggg attaccttcc tgttctcggt
     6181 gatgtctggc gtgatgtctc gctgcgaccg gatggcggag aacgccgata ctcgcggcat
     6241 tgaacgcgtg aactatctgg cactgctggc actcggcact ttctattttc tctgcgcttt
     6301 cctgcctatc tacttcggcg cggaacatgc aaaaaccatc attgatgtcc tgccgcaacg
     6361 attaatcgac ggcctcggcg tcgcgggcgg catcatgcca gcaatcggtt ttgccgtgct
     6421 gctgaaaatc atgatgaaaa acgtctacat cccctacttc atcctgggct ttgttgccgc
     6481 agcctggctc aagttaccgg tgctggctat tgctgccgct gcgctggcga tggcgctgat
     6541 cgacctgctg cgtaaatctc ctgaaccgac tcaacctgcg gcacagaaag aggaatttga
     6601 agatggcatc taatcaaacc accctgccga acgtcagtga aaacgaagaa acattgctga
     6661 ctggcgtcaa tgaaaacgtg tatgaagatc agagcattgg cgcggagctg acgaaaaaag
     6721 atatcaaccg cgtcgcctgg cgttccatgc tgttacaagc gtcttttaac tacgaacgta
     6781 tgcaggcttc tggctggttg tacggtctgc tgcctgctct gaaaaagatc cacactaata
     6841 aacgcgacct ggcgcgcgcc atgaaggggc atatgggttt cttcaatacc catccgtttc
     6901 tggtgacatt tgttatcggc attatccttg cgatggagcg ttctaagcag gacgttaaca
     6961 gtattcagag caccaaaatt gccgtcggtg cgccgctcgg cgggattggc gatgcaatgt
     7021 tctggctaac gctactgccg atttgtggcg ggataggcgc aagccttgcg ctacaaggct
     7081 ctattcttgg cgcagtcgta tttattgtgc tgttcaacgt ggtacacctg gggctgcgtt
     7141 ttggtctggc gcattatgct taccgcatgg gcgtggcggc gattccactc attaaagcga
     7201 ataccaaaaa agtcggccat gctgcgtcta tcgttgggat gacggtaatc ggcgcgctgg
     7261 tggcaaccta tgttcgttta agcaccacgc tggaaatcac agcgggcgac gcagtggtta
     7321 agttacagac tgatgttatt gacaaactga tgcctgcctt cttaccgctg gtctacacct
     7381 tgaccatgtt ctggctggta cgccgcggct ggagtccgct gcgcctgatt gcagttaccg
     7441 tggttctcgg catcgtcggt aaattctgcc atttccttta aatacaatga ggtattagat
     7501 gttaagtatt attttgacag ggcatggcgg gtttgccagt ggcatggaaa aagcaatgaa
     7561 gcaaatcctc ggcgagcaat cgcagtttat tgccatcgat tttcccgaaa cctcaagcac
     7621 cgcgctgctt acatcgcagc ttgaagaggc catcgcccaa ctggattgtg aagacggcat
     7681 tgtttttctg actgatttgc tgggcggcac gccgtttcgc gtagcttcaa cgctggcgat
     7741 gcaaaaaccg ggctgtgaag tgatcaccgg caccaattta caactgctac tggagatggt
     7801 gctggagcgc gaagggttaa gcggtgaaga gttccgtgtg caggcgctgg agtgtggaca
     7861 tcgtgggctg accagtctgg tagacgagtt aggccgctgc catgaagaat gtccggtcga
     7921 ggaaggaata tgacacccgt tctgcgcgcc agaaggctgc tgactgaaga gggatggctc
     7981 gatgaccatc agttgcgtat tgctgacggt gtcatcgcag caatcgaacc gattccagtg
     8041 ggcgtgactg aacgcgatgc ggaactgctc tgccctgctt acatcgacac ccatgtacac
     8101 gggggtgcgg gcgttgatgt aatggatgac gcgccggatg tactcgacaa gctggcaatg
     8161 cacaaggcac gcgaaggtgt cggcagttgg ttaccgacca ccgtaaccgc gccgcttaat
     8221 accattcatg cggcgctgaa acgtattgct caacgttgcc aacgcggcgg acctggtgcg
     8281 caagtgctgg ggagttatct cgaaggaccg tacttcacgc cgcagaataa aggcgcgcaa
     8341 ccgccggaat tgtttcgcga gcttgaaatt gccgagctgg atcaattgat tgccgtttct
     8401 cagcacacct tacgcgtggt agcgctggca ccggaaaaag agggggcatt gcaggccatc
     8461 cgccatctta aacagcaaaa tgtacgagtg atgctggggc atagcgcggc gacctggcaa
     8521 caaactcgcg ccgcgtttga tgctggtgcc gacggcctgg tgcattgcta taacgggatg
     8581 acaggtttac atcaccgcga accgggaatg gttggcgcgg gattaacgga caagcgcgcc
     8641 tggctggaac tgatagccga tggtcatcat gtgcatccgg cggcaatgtc gctgtgttgt
     8701 tgctgtgcga aagagagaat cgtactgatc accgacgcga tgcaggcagc tgggatgccg
     8761 gatggtcgct atacgttatg tggtgaagaa gtgcagatgc acggtggcgt tgtccgtacc
     8821 gcgtctggtg ggctggcggg caatacgctg tctgttgatg cggcagtgcg caatatggtc
     8881 gagttgacgg gcgtaacgcc tgcggaagcc atccatatgg cgtcgctgca tccggcgcga
     8941 atgctgggtg ttgatggtgt tctgggatcg cttaaaccgg gcaaacgcgc cagcgtcgtt
     9001 gcgctggata gcgggctaca tgtgcaacaa atctggattc agggtcaatt agcttcgttt
     9061 tgatggtttg ctccattatt gggccttcac ttcccccgta aggcctttct ttttctttct
     9121 ttttgatctg tgcagcggtg tcggatgcga cgctaacgcg tcttatgcga cctacggtac
     9181 gccgcatccg gcaatcggtg aacatagcga cgtcattacg caattcttta atcttccctt
     9241 gtcttttctt tcaacgatca cagatttcgt tttatttcct ttttcttcat tgaactttct
     9301 gtttcttttc tatagatttt aatcaacgaa agacatcacc aagtgaaatt aaacgaaagg
     9361 caagtgaaag cgacaacgcc cgacgtcaag ttcatcagac taaggattga gttatgccag
     9421 aaaattacac ccctgctgcc gccgcaaccg gtacatggac tgaagaagag atccgccatc
     9481 agcctcgcgc atggatccgt tcactcacca acatcgacgc gctacgttcc gcgctcaata
     9541 acttccttga accgttactg cgcaaagaga atctgcggat catcctgacc ggagccggaa
     9601 cctcggcatt tatcggtgac atcatcgcgc cgtggctcgc cagccatacc ggtaaaaact
     9661 tcagcgccgt accgaccacc gatctggtca ctaatccgat ggactacctg aacccagccc
     9721 atccgctgct gttgatctcc ttcggtcgat ccggcaacag cccggaaagc gtcgctgccg
     9781 tggaactggc aaatcaattt gtaccagaat gctatcacct gccgatcacc tgcaacgaag
     9841 cgggcgctct ttaccaaaac gcgatcaaca gcgataacgc gtttgccctg ctgatgcccg
     9901 cagaaacgca cgatcgcggc tttgcgatga ccagcagcat taccaccatg atggccagct
     9961 gcctcgcggt tttcgcacct gagacgatca acagccaaac cttccgcgac gtggcggatc
    10021 gttgccaggc gatcctgacc tcactgggcg atttcagcga aggtgtgttt ggttacgcac
    10081 cgtggaaacg gatcgtttat ctcggcagcg gtggcttaca gggcgcagca cgcgagtcgg
    10141 cgctgaaagt gctggaactg acggcgggta aactggcggc cttttatgat tctccaaccg
    10201 gattccgtca tggaccaaaa tcgctggtcg ataacgaaac actggtggtg gtatttgtct
    10261 ccagccaccc ttacacccgt cagtatgatc ttgatctgct ggctgaactc cgccgtgaca
    10321 accaggcaat gcgcgtaatc gccatcgccg cggaaagcac cgacatcgtc gctgccggtc
    10381 cacatattat cctgccgccg tcacgtcact ttatcgacgt tgagcaggca ttttgcttcc
    10441 tgatgtacgc ccagacgttt gcactgatgc agtcgctgca catgggcaat acgccggata
    10501 ccccatcagc cagtggcacc gttaaccgcg tggtgcaagg cgtaatcatt catccgtggc
    10561 aggcataaga ggatcgcatt atgagcatta tctccactaa atatctgtta caggacgccc
    10621 aggccaatgg ctacgcggtg cctgctttta acattcataa cgccgagacg atccaggcga
    10681 tcctcgaagt gtgcagtgaa atgcgatcgc cggtgatcct cgccggaacg ccggggacct
    10741 ttaaacatat cgcgctggaa gagatctacg ccctgtgcag cgcctattcc acaacctaca
    10801 acatgccact ggcgctgcat ctcgaccacc acgaatcgct ggatgatatt cgccgtaaag
    10861 tccacgcagg tgtgcgcagt gcaatgatcg acggcagcca cttcccgttt gccgagaacg
    10921 tgaagctggt gaaatcggtt gttgacttct gccactcaca ggattgcagc gtggaagcag
    10981 aactgggccg cctgggcggt gttgaagatg acatgagcgt tgacgccgaa agtgcattcc
    11041 tgaccgatcc acaagaagct aaacgctttg tcgaactgac tggcgtcgac agcctggcgg
    11101 tagcgattgg tacggcgcac ggcttataca gcaaaacgcc gaagattgat ttccagcggc
    11161 tggcggaaat tcgtgaagtg gtggatgttc ctctggtgct acatggtgcc agcgatgttc
    11221 cggatgaatt tgtccgtcgc actattgaac ttggcgtcac aaaagtgaac gttgccacag
    11281 aattaaaaat agccttcgct ggcgcggtta aagcctggtt tgcggaaaat ccgcagggta
    11341 atgatcctcg ttattatatg cgcgtcggaa tggatgcgat gaaagaagtt gtcagaaata
    11401 aaattaatgt ctgtggttca gcgaatcgaa tttcagcata atcattagtt ttttatgatt
    11461 tatcccaatg tacttcccga ttaatatcgg ggagtgcctt aatggaaaag gagatgacta
    11521 aaccttaata aatacatcag tacaatatcg caacaataat atattaaaaa aaatcatatt
    11581 attcaacttt atggtgagga ttacacaatg agcagtccaa atattctctt aacccgtatt
    11641 gataaccgtc tggttcacgg tcaggttggc gtgacctgga catccaccat cggtgcgaat
    11701 ctgctggtag tcgtggatga tgttgtcgct aacgatgata ttcaacagaa attaatgggt
    11761 attaccgcag aaacctacgg ctttggcatt cgtttcttta ctatcgaaaa aaccattaac
    11821 gtcatcggca aagctgcacc gcatcagaag atcttcctga tttgccgtac gccacaaacg
    11881 gtacgtaaat tggtagaagg tggcattgac ctgaaagata tcaacgtcgg caatatgcat
    11941 ttctcggaag ggaaaaagca aatcagcagt aaagtttatg tcgatgacca ggatctcgcg
    12001 gacttacgtt ttattaaaca acgtggcgtg aatgttttca ttcaggacgt ccctggcgat
    12061 caaaaagaac aaatccctga ctaaatctaa aatcgcctta atattggttt gaggtaataa
    12121 aaatgcatga aataacccta cttcagggat tatccctggc ggcgttagtt ttcgttctgg
    12181 ggattgattt ttggctggaa gccttatttt tattccgccc gataatcgtt tgtaccctaa
    12241 ctggcgctat tctcggtgat attcagactg gcttaattac cggtggtctg acagagttgg
    12301 ctttcgccgg attaacccct gcaggtggtg ttcagccgcc caacccgatt atggcgggtc
    12361 tgatgaccac cgtcattgca tggtctacgg gcgttgatgc caaaacagca attggtcttg
    12421 gcctgccgtt tagtttgtta atgcagtacg tcattctgtt cttctattcc gctttctcat
    12481 tatttatgac caaagccgat aaatgcgcga aagaggcgga tacggcagcg ttttcccggc
    12541 ttaactggac aacgatgctc atcgtcgctt cagcgtatgc ggcgattgct ttcctctgta
    12601 cttacctggc acaaggggcg atgcaggcgc tggtgaaagc gatgcccgcc tggttgaccc
    12661 acggctttga agtggctggc ggtattctgc ctgccgttgg ttttggcttg ctgctgcgcg
    12721 taatgttcaa agcgcaatat atcccttacc tgatcgctgg cttcctgttt gtttgctaca
    12781 tccaggtcag caacctgttg ccggttgccg tactgggtgc aggctttgcg gtgtatgagt
    12841 ttttcaatgc gaaatcccgg cagcaagcgc aaccgcagcc cgttgccagt aaaaatgaag
    12901 aagaggacta cagcaatggg atctgaaatc agtaaaaaag atatcacccg tctgggcttt
    12961 cgttcgtcgc tgctgcaagc gagctttaac tacgaaagga tgcaggcggg cggttttacc
    13021 tgggcgatgt tgccgatcct gaaaaagatt tataaggacg acaaaccggg cttaagcgcg
    13081 gcgatgaaag ataacctcga atttattaat acccacccga atctggtcgg attcctgatg
    13141 gggttattaa tttcgatgga agaaaaagga gaaaaccgcg acactattaa aggcctcaaa
    13201 gtggcactgt ttggcccaat tgccgggatt ggcgatgcga ttttctggtt tactttgttg
    13261 ccgattatgg cgggaatttg ctcatcattt gccagccagg gaaacctgct ggggccgatt
    13321 ctatttttcg ccgtttacct gcttatcttt ttcctgcgcg tcggctggac ccacgtcggt
    13381 tattcagtcg gcgtgaaggc gatcgataaa gtgcgagaga actcgcagat gattgctcgc
    13441 tcggcaacca tcctcgggat cacggtaatc ggcgggctga tcgcttcgta tgtgcatatt
    13501 aacgtggtga catcgtttgc catcgacagt acccacagcg tcgcgctgca acaggatttc
    13561 ttcgataaag tcttcccgaa cattttaccg atggcctaca ccctgctgat gtattacttc
    13621 ctgcgggtga aaaaagcgca tccggtgctg ttaatcggcg tgacttttgt gctctctatt
    13681 gtttgttccg cattcggcat tttgtaaatg gaacgaggca ctgcgtctgg cggtgcctct
    13741 ttacttaagg aatttcatcc tgtgcaaacc cttcagcaag ttgaaaacta tacggcgtta
    13801 agtgaacgtg ccagcgaata tttattggcc gtgatccgta gcaaaccgga tgccgtgatt
    13861 tgcctggcga ccggagccac gccattactg acgtatcatt atctggtaga aaaaatccac
    13921 cagcagcagg ttgatgtcag ccagctcacc ttcgtgaagc tcgacgaatg ggtggatctg
    13981 ccattaacga tgcctggtac ctgcgaaact ttcctgcaac agcatatcgt gcagcagctg
    14041 gggctacgtg aagaccagct catcagcttt cgctccgaag agataaatga gacagagtgc
    14101 gaacgggtaa cgaacctgat tgcgcgcaaa ggcggtctgg atttatgcgt tctaggattg
    14161 gggaaaaacg gtcatcttgg gctgaacgaa ccgggagaaa gcctacaacc ggcctgccat
    14221 atcagtcaac ttgatgccag aacacagcaa catgagatgt taaaaaccac gggtcgcccc
    14281 gtgactcgtg ggatcacctt aggcctgaag gatattctca atgcccgcga ggttttgtta
    14341 ttggtgactg gcgaaggaaa gcaggatgcg acagaacgtt ttctcacggc taaagtctcc
    14401 accgctatcc cggcttcgtt tttatggctg ca
//
LOCUS       DAZFXT010000116        14393 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.116, whole genome shotgun sequence.
ACCESSION   DAZFXT010000116 DAZFXT010000000
VERSION     DAZFXT010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.116"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(125..793)
                     /gene="artM"
                     /locus_tag="ACHKW0_003776"
     CDS             complement(125..793)
                     /gene="artM"
                     /locus_tag="ACHKW0_003776"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HFO7476534.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(793..1509)
                     /gene="artQ"
                     /locus_tag="ACHKW0_003777"
     CDS             complement(793..1509)
                     /gene="artQ"
                     /locus_tag="ACHKW0_003777"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:0071705 - nitrogen compound transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HFO7476535.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(1516..2247)
                     /gene="artI"
                     /locus_tag="ACHKW0_003778"
     CDS             complement(1516..2247)
                     /gene="artI"
                     /locus_tag="ACHKW0_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein ArtI"
                     /protein_id="HFO7476536.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(2265..2993)
                     /gene="artP"
                     /locus_tag="ACHKW0_003779"
     CDS             complement(2265..2993)
                     /gene="artP"
                     /locus_tag="ACHKW0_003779"
                     /EC_number="7.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_process: GO:0003333 - amino acid transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HFO7476537.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(3211..3726)
                     /gene="ybjP"
                     /locus_tag="ACHKW0_003780"
     CDS             complement(3211..3726)
                     /gene="ybjP"
                     /locus_tag="ACHKW0_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HFO7476538.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPES
                     ARVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            3852..4175
                     /gene="ybjQ"
                     /locus_tag="ACHKW0_003781"
     CDS             3852..4175
                     /gene="ybjQ"
                     /locus_tag="ACHKW0_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HFO7476539.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            4172..5002
                     /gene="amiD"
                     /locus_tag="ACHKW0_003782"
     CDS             4172..5002
                     /gene="amiD"
                     /locus_tag="ACHKW0_003782"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HFO7476540.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQIDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(4999..6012)
                     /gene="ybjS"
                     /locus_tag="ACHKW0_003783"
     CDS             complement(4999..6012)
                     /gene="ybjS"
                     /locus_tag="ACHKW0_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7476541.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(6111..7541)
                     /gene="ybjT"
                     /locus_tag="ACHKW0_003784"
     CDS             complement(6111..7541)
                     /gene="ybjT"
                     /locus_tag="ACHKW0_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HFO7476542.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(7552..8553)
                     /gene="ltaE"
                     /locus_tag="ACHKW0_003785"
     CDS             complement(7552..8553)
                     /gene="ltaE"
                     /locus_tag="ACHKW0_003785"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HFO7476543.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(8590..10308)
                     /gene="poxB"
                     /locus_tag="ACHKW0_003786"
     CDS             complement(8590..10308)
                     /gene="poxB"
                     /locus_tag="ACHKW0_003786"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0052737 - pyruvate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HFO7476544.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(10441..11409)
                     /gene="hcr"
                     /locus_tag="ACHKW0_003787"
     CDS             complement(10441..11409)
                     /gene="hcr"
                     /locus_tag="ACHKW0_003787"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HFO7476545.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(11421..13073)
                     /gene="hcp"
                     /locus_tag="ACHKW0_003788"
                     /gene_synonym="priS"
     CDS             complement(11421..13073)
                     /gene="hcp"
                     /locus_tag="ACHKW0_003788"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HFO7476546.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(13217..14116)
                     /gene="lysO"
                     /locus_tag="ACHKW0_003789"
     CDS             complement(13217..14116)
                     /gene="lysO"
                     /locus_tag="ACHKW0_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HFO7476547.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         3477 a         3852 c         3804 g         3260 t
ORIGIN      
        1 gcactatgta ggccggataa ggcgtccacg ccgcatccgg cacaggcacc gtgctgatgt
       61 ctgatgcgac gctggcgcgt cttatcagac ctacaaaccc ccccggcgaa tgtacgcagc
      121 tacattaatt tcgccgttcg aatgccagtg ctttgcgctc gatcagacgc atcatcagcg
      181 tcagcaggcc gttgacgacc aggtaaataa tccctgccgc accgaacacc attacatcgt
      241 aggtgcgtcc gtacaacaac tggctgtatc ccatcacttc catcagcgta atggtgtaag
      301 ccagagaggt acttttgaac accagcacca cttcgttgga ataagaagag agcgagcgtt
      361 taaaggcata cggcagcagg atcgccagcg tatctttttt gctcattccc agggcgctac
      421 aggactgcca ctgaccttct gggatcgcac gaattgcacc gtaaaacagc tgcgtggtat
      481 acgccgcact gttcagcgat aacgcaatca gcgcacataa ccacggttct gacaacaaat
      541 gccacagtgc cggatactcc tgcaaagtcg gaaactggcc cggcccgtaa taaatcagga
      601 agatctgcac cagcagcggc gtaccggtaa acagcgtgat ataaccccgc accagccaca
      661 ccagcaccgg cgttttcagc gtcaggatga tggtaaaaat caatgccaga atcagtgcca
      721 caatcagcga ggcaacggtt agcgtcaggc tggtgtgtag ccctttcatc agttcgggta
      781 aatactcaaa cattagctgg gcctccgctc aaaacgtgtc gcgcgcaggt caatgcgttt
      841 gagaatgtac tgactgagca gggtgatcac caggtaaatc gccgccgcca caatgtacca
      901 ggtaaatggt tcctgggtac gagtagcgat gctttttgtt tgcagcatta aatcattcac
      961 actaatcaaa ctgaccagcg cggtatcttt cagcagcacc agccactggt taccgaggcc
     1021 aggcagcgca tgacgccaca tctgcggcat caccagacgg aaaaagatag ccgatttcga
     1081 cagccccagc gcctgaccag actcccactg acccaccggc accgctttca acgcgccccg
     1141 aagcgtttgc gaggcatagg cggcatacag cagtgacaga gcgatgacac cacaaaggaa
     1201 cgggctaacg tcgaagttct caatgtccat ctgcactggg atctgcacga acccaagatt
     1261 gatagtgaag ccatccgaaa gcgtcagcag cagctgcgag gagccaaaat agataaacag
     1321 caccaccaga atttctggca ggccacgcag aatggttacc agcgctgaac ctgcccacgc
     1381 gacaggacgc cattttgccg actcccatac cgcaaagaac atcgccagcg ccagcccgac
     1441 aatcaatgca caaacggcaa ggccgacggt catcccggcg gcgcttgcta aaggaaaaaa
     1501 ttcattcatc aggaattact tctggaacca tttgttgtag atggtttcgt aagtgccatc
     1561 tttcttcact ttttccagcg cagtgttgag tttctgctgc agctcagtgt tgccctgacg
     1621 taccgcgatg ccgaggccag tgccgaagta atctttatcg gtcactttgt cgcccaccgc
     1681 cgccagtttc gggttatctt tcagccactc agtgaccact gcggtgtcac cgaagacgcc
     1741 gtcgatacgc ccgttttgca gatccagttt tgcgttctgg tagctgtcat acggaacggt
     1801 agtgatttcc gggtgcttat ccataatgaa tttctggtgt gtcgtcccgt tctgaacgcc
     1861 gacttttttg cctttcagct gatcaacact ggtgtatttg ccttgctgac ccacaaacag
     1921 ggcagagttg tcatagtacg gggtggtaaa cagtacctgc ttttcacgct ccggagtgat
     1981 atccatgccc gccatcactg cttctacgcg acggaatttc aggcttggga tcaggctgtc
     2041 aaacgcctgg ttagagaaag tacaggttgc atcaatctct ttacacagtg cctgtgccag
     2101 gtcgacgtca aaaccaacga tctggttgtt tgcatcaatc gattcaaacg gaggatagga
     2161 ggcttcggta gcaaaacgaa tggtttcggc agctgtggcg gaaagactaa aacctgcaat
     2221 taacgcggca atcagaactt ttttcattgt tgttatcccg aatcttagtg agagagatag
     2281 tttttaaatg cttcggtttg cggctcggta aagcagctcg cgtcgccttg ttctacgata
     2341 tgaccatttt ccatatacac cactcggctg gcggttttac gcgccacttc aacttcgtgg
     2401 gtgacgatca cctgggtaat attcgtttct gccagctcac gaatgatgct gacgatttgt
     2461 gccgtaattt ccgggtccag tgcggcggtc ggttcatcga acagcagtac ctgcggttcc
     2521 atcatcaacg cacgggcaat agcaacacgc tgctgctgac caccagaaag atgcagcggg
     2581 taacgatcgc tataaggttt gagacgcaga cgttccagca gtttttctgc acgggccagc
     2641 gcctgatctt tactcaaccc cagtacacgg cagggcgctt caatcaggtt ttgctgcacg
     2701 gtcagatgcg gccacaggtt gtattgctga aacaccatgc caacgttacg acgcaaatcg
     2761 cgaatcgctt tgtcagaggg tgttttggtg aaatcgaaat ggttgcctgc aatgttgagc
     2821 gtaccggagc gcggcatctc aagcagattg agtacacgca gcagcgagct tttacccgcg
     2881 ccgctggggc caagtaacac cagcgtttcg ccctgtgggc aatccagcgt gatatcgaac
     2941 agcgcctgat gcgcgccgta gaagcaatta atgccgttta attgaatact cattgacact
     3001 cgtatactgg cagtctgata gctattgagg tcgaagatag tacctttgac agaataatta
     3061 tgcaatattt ctgctttaaa agttaaaagc aaagcgcatt attcaataaa catagcacaa
     3121 aataacgggg gcggtggtcg gtgagcataa atgtcggcat tcctcacgaa atgccggaca
     3181 atttacgggg tttattggtt gatcaaggcg ttagcgattc tcgatggact gacggagcgt
     3241 acccgctgtg gcatgaacgc tgccgcctaa atagcgtaca tcgtcgatca cccaacactg
     3301 gccttcctga atcattaaca cttcatcctg ccagctctga tcaccctgtt ttagatccac
     3361 acgcagagga atatttcgcg catcacgatt cgggattgtt gaggcactgg caacacgggc
     3421 gctctctggc aaggttgtgc gactggagaa tggatcgttg gtcagtagtt cccgatggtt
     3481 attatcccgg gaggcatcgc taagcagtgt cgccagtttg tcgctcagat aagggcgcaa
     3541 ggcggtgatg tcgttgctgc ggtgcaaaat gcggtagtca taaaattgct gggccacgtt
     3601 atccgggcct ccttcaacgc aaggaccact gcgtgtgccg ttatctttat aagctggagt
     3661 gactgtggtg caggcactga ggagcagtgc gcaggggata agcattgtca atttgctgta
     3721 gcgcataatg atttccttat aagcgatcgc tctgaaagcg ttctacgata ataatgatat
     3781 cctttcaata atagcgtatc agtctgataa tgctttagag atcgaaggct taacaaacaa
     3841 ggagatcgat catgcaattt tctacaaccc caactctgga aggccagacc atcgttgaat
     3901 attgcggtgt ggtgaccggc gaagcgattt taggtgccaa tattttccgt gatttctttg
     3961 ccggtatccg cgatatcgtt ggcggacgtt ccggtgcgta tgaaaaagaa ctgcgtaaag
     4021 cacgggagat cgcctttgag gaattaggct cccaggcgcg ggcgctgggg gccgatgccg
     4081 tcgtcggtat tgatatcgac tacgaaacgg tcgggcaaaa cggcagtatg ctgatggtta
     4141 gcgtcagcgg tacggcggtg aaaacgcgtc gatgagaaga gtcttctggc tgatcgctgc
     4201 cgctctgtta ctggcagggt gtgcaggcga aaaaggcatt gtcgagaaag agggatatca
     4261 gattgatacc cgacgccagg cgcaggcggc gtatccgcgc attaaagtgc tggtgatcca
     4321 ctacaccgca gatgattttg atagctcgct ggcgacactg accgataagc aggtcagctc
     4381 gcattatctg gtccctgcgg taccaccgcg atacaacggt aaaccgcgca tctggcaact
     4441 ggtgccggaa caagaactgg cctggcatgc ggggattagc gcctggcgcg gggcaacgcg
     4501 ccttaacgac acctctattg gcattgagct ggaaaaccgt ggctggcaaa aatcggccgg
     4561 agtgaaatat tttgccccgt ttgaaccggc acagattcag gcgcttattc cactggcgaa
     4621 agatattatt gcccgttatc acatcaagcc ggaaaacgta gtggcacatg cggatatcgc
     4681 accgcagcgc aaagacgatc cggggccatt atttccctgg cagcaactgg cgcagcaggg
     4741 gattggtgcc tggccggatg cgcagcgggt taacttttac cttgccgggc gcgcgccgca
     4801 cacaccggta gataccgcgt cattgctgga gcttttggcg cgctacggtt atgacgttaa
     4861 acctgatatg acaccgcgcg agcagcggcg cgtgattatg gcattccaga tgcatttccg
     4921 cccgacgtta tataacggcg aagcggatgc agaaactcag gcgattgccg aagcattgct
     4981 ggagaaatac gggcaggatt agcgcggcag ttttccgtgg tcgcgtagcc aggcggcagt
     5041 tttctcgata ccttcatcca gggtgatgac cggctgataa cctaactctt cctgcgcacg
     5101 cgtaatatcc agcgtaaagt caaaattcaa cttggagacg ccgtagtggg tcagcggcgg
     5161 ctcttttgct gacttgcggc ctaaacgctc catgctgcgg gcgatcatat ccagcatcgg
     5221 gtaggggacg gaacgaatac gacagtcaat attcaactcg tcgatcagct tctgcacgat
     5281 gctgcgcagt gtgcgatgct cgccgttggt gatgttgtac acacgcccgg aaggtagctt
     5341 atcgcaggct tcctggcttg ccagccacat tgcgtgcacg gcattttcat agtaggtcat
     5401 atcgaccagc gcactgccgc catgcggtaa cagaatactg ccgtagtggt gcatcatatg
     5461 cgccagacgg ggaataaaga ctttatcgtg cggtccgaac agactttgtg ggcgcagaat
     5521 agtaaagcgc gtttgtggat tcgcctgcga aagcatattg atcacttctt cgctggctgc
     5581 tttgctgcgg gcaaactcgt tggcgaagcg gtgagggcga aaatcttctt taatatcgcg
     5641 atggtggtga taatcgaagt acagggaggg ggaagagata tgaataaagt tacgcacacc
     5701 ccaggcgaca gcccattcac ccaggcggcg agtggcgcga acgttagcca gatcgaaagc
     5761 ctgttgtgtc ccccagggtg aggtaaagct ggagcagtgc cacagcgtat caatgcccgc
     5821 gagcatcact ttagcttgtg atgaaaccag ctcggtcaga tccgccggaa caaactctgc
     5881 gcccattttt tccagcaatt tgcccattgc ctcgttgcga ccggtcgctc gcacgctgat
     5941 gcctttctgg cataaaaact ctaccgcgtt tcgacctaag ccgctggtgg cgccggtaac
     6001 cagtaccttc atatcaatcc actgttgttg agaaaataac gtgcgcattc ttccgtgatt
     6061 tcccccatga tgcaatggga aacatgaaag aataacgcag gttttgtcga ttaatctgtg
     6121 ctttgttctg ccagtctggc gatttgtttt gccattccgc gaaaaataaa cagatgcgcg
     6181 gggatcatca ataaccagta aaacagcccc ggcataccgt gcggatgcca gaaagcgcgg
     6241 acatcgatag tacgatagtc gcctttatct tccaggctaa aacacagtcg tcccagcccc
     6301 ggcgctttca tgccaaataa caacgtaagt tgtttttccg gttcaacgac aatcactttc
     6361 cagctatcta ccgcatcgcc agtctgtaaa tattcgcgct ccgggcggcc tttcgccagc
     6421 ttatgaccga tcgcgcggtc catcaacgcc cgtgtctgcc acaaaatatt gccaaagaaa
     6481 taacgctctt taccgccgat ttggttcact acctgccata aagcagcaag gctggcggac
     6541 gttttaacgg taaaccccgc ctgtttggca aaataaccgt actccggtcg ccagcgggca
     6601 aaggcctgag cgtcgtagcc ccagtcgctg gagttgacca gtttttcctc ctctttcaac
     6661 gtgctacgta ccgcgtcatc gaaagcgatc agccgttgtg ggatgagtgc acgtagcgcg
     6721 gtatcatccg ccagcagatc gtgtttcagc ccctgaatca acgccctggc ggtggtgggc
     6781 ggtacggaag taatcacatt gagaaaccac accgaaatcc agcgggtggg gagggggatg
     6841 gggatcaacc agcggcgctt accgctcacc gccataaaat gttcaaactg ttgctgataa
     6901 ctgagcacct ctggtccggc ggcttcgaag atgcggtgtt cgctggccgg atgatctaac
     6961 aacgccacca gatagtgcag caagttttcc agcgcgatgg gcgtggtgcg tgaacgtacc
     7021 cagcgtggcg gcgttaacac tggcaggttg tagaccatat cgcgcatgac ttcgaacgcc
     7081 gctgaacctg cgccaacgat aattccggcc cgaagttcgg tcacaggtac attcgcttca
     7141 cgaagaatgt ccgccgtagc ctgacgagca cgcagatgat ccgactgctc atgtggcggg
     7201 gcctgcaacg aactgagaaa gattaattgc ttaactggta cttcacgtag cgcatcgcgg
     7261 acgttgagag ccacctggcg ctcctgagcg ataaaatcgc cgccttcgcc catgctgtgc
     7321 accagaaaat agacggtatc gatatcctgc aacagggccg gaaggttatc cggccagctg
     7381 agatcgactt tatggcaact gacgtttgcc agttgcagct ttgcaagcct gtcgacatga
     7441 cgtgccgccg ccaggatctg atgcccttgc tggctgagtg tgcgcaccag atgctgacca
     7501 atgtagccac tggcaccgag aactaaaatg cgttgcggca cgtctctctc cttaacgcgc
     7561 caggaatgcg cgccagtggg cggcgacttc cgccagttgt tcgcgcgaga cgtcaagatg
     7621 cgtcaccagg cggacaatcg gcgaggcgtt aatcagcacg tttctcgctt tcatgtattc
     7681 gcctaacgcg gcagcatttt cttccccgac gcgaacaaac agcatattag tgtcctgacg
     7741 catcacatcc gcgcctgctt cacgcagttg ctccgccatc caggcggcgt tgtcgtgatc
     7801 ttcctgcaac cgtgcgacgt tatttttcag ggcatacatc ccggcggcag ccagaatccc
     7861 ggactgacgc atcccgccac cggccatttt ccgccagcgg atggcacgtt taatgtaatc
     7921 acgattaccg acgagtaatg aaccgactgg cgtcccaaga cctttcgaca ggcaaatggt
     7981 gaacgaatca caatattgcg tgatctcttt cagttcgcag ccgtaagcca ccacggcatt
     8041 aaagatgcgc gcaccgtcaa catgcagcgc cagattgcgc tcgcgggtaa attcccatgc
     8101 ttctttcagg tattcacgcg gcagcacttt gccgttgtgg gtgttttcca gactgagtaa
     8161 tttggtgcgg gcgaaatgga tatcgtcggg tttgattttc atcgccactt tatccagcgg
     8221 tagcgtgccg tcggcagccg cgtcgatggg ttgcggctga atactgccca gcaccgctgc
     8281 gccaccggct tcaaacagat agttatgcgc ggcctgaccg acaatatact cttcgccacg
     8341 ttcgcagtga ctgagcagag cgaccaggtt ggcctgagtg ccggtaggca gaaaaatggc
     8401 ggcttcttta ccggaaagct ctgctgcata gtcctgcaga gcattaacgg tagggtcgtc
     8461 tccgtaaacg tcgtccccaa ccggggcggc catcatcgct tcgagcatgg cgcggctcgg
     8521 tcgggtaacg gtatcactgc gtaaatcaat catggcatgt ccttattatg acgggaaatg
     8581 ccaccctttt taccttagcc agttcgtttt cgccagttcg atcacttcat cgccgcgtcc
     8641 gctgataatt gcgcgcaaca tatacaggct aaaacctttg gcctgttcga gtttgatctg
     8701 cggtggaatg gctaactctt ctttggcgac caccacatcc accaacaccg gaccgtcgat
     8761 agagaaggcg cgttgcaggg cttcatcaac ttcagacgct ttttctacac ggatacccgt
     8821 aatgccgcac gcttcggcaa tgcgggcaaa gtttgtgtcg tgtagttcgg tgccgtcagt
     8881 caaatagcca ccagctttca tctccatcgc cacaaagccc agcacgctgt tgttaaagac
     8941 gacaattttc actggcagtt tcatctgcac tactgagagg aaatcgccca tcaacatgct
     9001 aaaaccgcca tcgccgcaca tggcgaccac ctgacgttct ggctctgtcg cctgcgcacc
     9061 cagcgcctgc ggcatggcgt tagccatcga accgtggtta aacgaaccta acaggcgacg
     9121 cttgccgttc atttttagat aacgtgccgc ccacaccgtt ggcgtaccaa cgtcacaggt
     9181 gaaaatagcg tcatcggcgg caaaatgact aatttgctgc gccagatatt gcgggtgaat
     9241 ggctttctcg ctcggtttag ctaaatcgtc cagccctttg cgggcgtcgc ggtaatcttc
     9301 cagcgcttta tccagaaact tgcgatcggc tttttcttcc accaatggaa gcaatgcacg
     9361 cagagtcgac ttgatatcgc cgaccagtgc catatccacc ttgctgtgag cgccgatgct
     9421 ggctgggttg atatcaatct gaatgatttt ggcatcggtc gggtagaagg cgcggtaggg
     9481 aaattgcgtg ccgagtagca ctaacgtgtc ggcgttcatc atggtatgga aacctgacga
     9541 gaagccgatt aacccggtca ttccaacatc atacggatta tcgtattcga catgttcttt
     9601 accgcgcagg gcatgaacaa taggcgcttt aattttcccg gcaaactcaa ctaactcttt
     9661 atgcgccccc gcgcagccgc tgccacacat cagggcgata ttgctggaat aacgcagcag
     9721 ttgcgccagt ttgcgtaact cttcttcttc cggcgtcacg actggttgtg gcgcatgata
     9781 ccagtgcgtg gttgcccctt ctggcgcagg ttttaacgcc acgtcgcctg gtaacacgac
     9841 aaccgaaacg ccacggttaa gcaccgcttt gcgcatggca atcgccagta cttgtgggat
     9901 ctgctccggg ctggaaacca actcgcaata gtgactacat tcgcggaata gctcttgtgg
     9961 gtgggtttcc tggaaatagc cgctgccaat ttcgctggag ggaatatgag cggcaatcgc
    10021 cagtaccgga acgtgattgc ggtggcaatc gaacaggccg ttgattaagt gcaggttgcc
    10081 ggggccgcac gatccggcgc agaccgccag ttctccgcta agttgtgctt cagcgccagc
    10141 ggcaaaggcc gccacttctt cgtggcgggt ggacatccac tcgatggtgc ccatgcgatt
    10201 aagactgtca ctaagaccgt tcagagagtc gccagtgact ccccagatgc gtttcacccc
    10261 tgccgattcg agtgttttgg cgatataagc tgcaaccgtt tgtttcatgg ttctccatct
    10321 cctgaatgtg ataacggtaa caagtttagt tcatctgacg gagggggaag ggatgggaga
    10381 gaaaggaggc actaacggtt aaatagcccg atgaaaggaa tatcatcggg cataaggcga
    10441 ttatgcgaga accaaatccc cctgcggatg gcaggagcag gccagtacgt aaccttcagc
    10501 gatttcggcg tcggtcagcg tcattgtact gctcaccgta tattcaccgg aaaccacttt
    10561 tgtcttacag cagccgcaaa cacccgcacg gcaggcagcg acaaccggaa cgttattgct
    10621 ttccagcgcc tccagtagtg tggtgccaac cggggcgtaa aattctcgtg ccggttgcag
    10681 tttggtgaat ttcagaccgc tggtcgccgc ttctgctact ggggtgaaga atttctcttt
    10741 aaagaaacgc gtcacgccga gcgctttcac ttcctgctct acccaatcca tatacggagc
    10801 cgggccgcag gtcatcacgg tacgtgaagc taagtcaggt acacctgcca gcagttcgcg
    10861 agtgagacga ccagcgataa agccttcggt aacgttattt tctgccacca gcgttaccgg
    10921 atagttacgc cactcatcgg cgaaaataac atcctgcggc gtacgcacgt tgtagatcac
    10981 ccgcacatcg gcctgtggac ggttcttcgc aagccagcga cgcatcgaca taatcggcgt
    11041 gacgccgcag cctgccgcca gcaacaggaa tttatcttct gctttatcgt cgcaggtaaa
    11101 ttcccccatc gcgtccgaaa gccagagata atcaccgcgt tttacatcgc gcgtcagcca
    11161 ctgggagccg acaccgtcat caatccgccg cacggtcagg gtgatatatt cactcacgcc
    11221 tggcgtggag gaaatggtgt aagcacgcag cgtttccgct gagtttcgca cgctgaccag
    11281 tgcatattgc ccggcgcgat atgggtagta atcgtggcaa atcagggaaa tcgtccacac
    11341 atccggcgtt tcttgcgtaa tgtgatgaac ctgcatccgc cacgggcatt gattcgttgg
    11401 catcgtcatc gacaaactcc ttacgcgctc aacagttgct tcatgtcttc ttcaacagtg
    11461 gtgatagaac gcaggccaaa tttctcgttc agcaccgcca gcaggtctgg tgtcaggaaa
    11521 ccaggtgcag tcgggccggt gacgatattt ttcacgccca gagaaagcag cgtcagcaga
    11581 atgacgatcg ctttctgttc aaaccaggag agcaccagcg acagcggcag atcgttgaca
    11641 ccgcagccca gtttctctgc cagagtgaca gccagaataa tcgctgagta agcatcgtta
    11701 cattgacctg catctaccag acgcggcaga ccttcgatat cgccaaactc aagtttgtta
    11761 aagcgatatt taccacaggc gagggtcagg atcaggcagt catccggcac gctggtggcg
    11821 aaatcggtga agtagtggcg ctcgccgcgt gcgccgtcac agccaccaag caggaagata
    11881 tgacgcagtt tttcacggct caccagatca atcagcgtat cagcagcgcc aagcagcgtc
    11941 tggcgaccaa aacccacggt gatcaggtgc ggaatttcgc tgtacgggaa gcccgccatt
    12001 tgttgcgcct gggcgatgac cgcagaaaaa tcttcgccat ccagatgacg cacacccggc
    12061 cagccaacga tgctgcgggt ccagatacga tcgtcataag cgcctacggt tgggtcgatg
    12121 atgcagttcg aggtcatcac gatggggcca gggaaacgag cgaactccac ttgctgattc
    12181 tgccagccac tgccgtagtt accgaccaga tgcttgaatt tacgcagctc cgggtagcca
    12241 tgcgcaggca gcatttcgcc gtgggtgtag acattaacgc ccgtgccttc ggtctgttcc
    12301 agcaggttgt agagatcttt gagatcgtga ccggaaatca gaatgcattt acccgccgtc
    12361 gctttgacgt tgacctgggt tggcgtcggg tgaccgtatt taccggtttc gcctgcatcc
    12421 agaatgctca tcactttgaa gttcatctgg ccgatttcca ttgaacactc aagaagcgcg
    12481 ttcatatcgg caggccaggt ccccagccac gccatgattt tatggtactg ggcataaata
    12541 tcgttgtcgt attgaccgag aacatgcgcg tgttccatat aggccgccgc acctttcagg
    12601 ccatacaggc acagcagacg caggccgaga atgttttcgc caatcgccgc tttatctttg
    12661 ttaggggtaa attctgctgc ctgacgttgc agctcgccga gatcgtcgct caccagttgc
    12721 aggtcagcca tcgggttatc gacgcgcgcg ttggcatcta cagccaggca ttgcgctttc
    12781 agcgcctcgc gcagggcaat cgcttcacga gcgtagccga caatacgcgg agaatcgaag
    12841 ttaacgttgg tcagggttga gaaaaaggca cgtggcgcga agctgtcaac atcgtggttg
    12901 atgatgccgt attcacgcgc ttttaccgcc caggcagaaa gcccttgcag cgccgcgatg
    12961 agtaaatcct gaaggtcaga agtttccgcc gttttaccac acatcccctg cgcgtatgag
    13021 cagccgtttc ctgccggagt acggatagtt tgttcacatt gcacacaaaa catgatcaca
    13081 ccttttaaag ttatatttaa tatacatgtt taaggttaag acgcttaacg cggggataaa
    13141 agggattttt catgcaactt taagggagat tgatttagcg caattttggc ggcagggatc
    13201 taccgccaga gaggtattac gcagagaaaa aggcgatgag gatcggcact aacaggctaa
    13261 gaataaaacc gtgaacaatt gccgccggga ccatatccag cccgccagta cgttgaagaa
    13321 cgggcagggt gaaatccatt gatgtggcac cgcataagcc cagtgcagta gagcggctgc
    13381 ggcgaatcag cccagggatc aacataatag caatcagttc acgggccaga tcattaaaaa
    13441 acgccgcgct cccgattacc ggaccaaaag attcggtcaa taaaataccg gaaagagaat
    13501 accagccgaa accggaggcc attgccagcg cggtattgat ggggagatca agaataaagg
    13561 cgttaattaa accaccaatt aatgaactga caaccaccac cacggcgaca atcattcccc
    13621 ggcgattaag gacaatctgc tttaaggtca tgccattatt gcgcaactga ataccaacga
    13681 ggaaaagtag caaaattaac gtgtattcac tggcttcggt cgcgtgttgt aagaaagcca
    13741 gtccactcag accaatggca aaaccaatca ctactacgcc gcacagtttt agcgactcca
    13801 gcgccatcgc aatacgcgac gggagttttt cttgctgatg gtggttgcgc cacggcaggc
    13861 ctcgctccag ccacatcagg gcggcaatat tacacagtaa aataacggta atactgacgg
    13921 cagaataatg cagaatcgcc aacaggttac tggcgaggtt atcgagaaac gccagactga
    13981 tacccataaa aaagagaata aggtaaacca tccagcttaa tagctgatta ataactttta
    14041 acgcagcttg ttggcgaagc ggaatgaggt aacccacaat caggggaacc agaatgatta
    14101 acagcccaga aaacatgaaa acccagtcct tgcaaagatg aagtcgaaat gcgcgatgac
    14161 acactactga aagcggaagg acgagtaaag ttgcaattaa aaggaaatgt tatgcataag
    14221 gagcagtaga gtattcgttt tcatttaaag atattcttgc gctttaatta caaactgcac
    14281 cgatgttggt ggcgtcaaaa tcgccgaggc gttccctgaa ggccggggca gcccacatgg
    14341 atgtgggctg agggcgcgtt ttacagggat gttacctcgc gcccgacccg gta
//
LOCUS       DAZFXT010000117        14211 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.117, whole genome shotgun sequence.
ACCESSION   DAZFXT010000117 DAZFXT010000000
VERSION     DAZFXT010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14211
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.117"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..552
                     /locus_tag="ACHKW0_003790"
                     /pseudo
     CDS             1..552
                     /locus_tag="ACHKW0_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(795..1490)
                     /locus_tag="ACHKW0_003791"
     CDS             complement(795..1490)
                     /locus_tag="ACHKW0_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HFO7476548.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTALNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1483..2910)
                     /locus_tag="ACHKW0_003792"
     CDS             complement(1483..2910)
                     /locus_tag="ACHKW0_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HFO7476549.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEANGESFRSWFKKHLAQEKKNG"
     gene            complement(2921..3640)
                     /locus_tag="ACHKW0_003793"
     CDS             complement(2921..3640)
                     /locus_tag="ACHKW0_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HFO7476550.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(4167..5021)
                     /gene="rclR"
                     /locus_tag="ACHKW0_003794"
     CDS             complement(4167..5021)
                     /gene="rclR"
                     /locus_tag="ACHKW0_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine-specific transcriptional
                     regulator RclR"
                     /protein_id="HFO7476551.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            5247..6572
                     /gene="rclA"
                     /locus_tag="ACHKW0_003795"
     CDS             5247..6572
                     /gene="rclA"
                     /locus_tag="ACHKW0_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance oxidoreductase
                     RclA"
                     /protein_id="HFO7476552.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHNENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILHDQIFTHPSMSESLNDLFSLVK"
     gene            6681..6917
                     /gene="rclB"
                     /locus_tag="ACHKW0_003796"
     CDS             6681..6917
                     /gene="rclB"
                     /locus_tag="ACHKW0_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine resistance periplasmic protein
                     RclB"
                     /protein_id="HFO7476553.1"
                     /translation="MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLE
                     DTIADIARKYNASSWKVTSMRIDNNSTATAVLYK"
     gene            6929..7522
                     /gene="rclC"
                     /locus_tag="ACHKW0_003797"
     CDS             6929..7522
                     /gene="rclC"
                     /locus_tag="ACHKW0_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HFO7476554.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7682..8551)
                     /locus_tag="ACHKW0_003798"
     CDS             complement(7682..8551)
                     /locus_tag="ACHKW0_003798"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HFO7476555.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLAKFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            8800..9657
                     /locus_tag="ACHKW0_003799"
     CDS             8800..9657
                     /locus_tag="ACHKW0_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7476556.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRV
                     YRHRDYWDLANIFPSFLIRQQQKTECRLVNFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMNNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKQTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNENFVEIDSNNIVC
                     EIFIPVRPV"
     gene            complement(9778..14031)
                     /gene="fdeC"
                     /locus_tag="ACHKW0_003800"
     CDS             complement(9778..14031)
                     /gene="fdeC"
                     /locus_tag="ACHKW0_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin FdeC"
                     /protein_id="HFO7476557.1"
                     /translation="MSRYKTGHKQPLFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     AHAQHAVQPRLSMENTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRMLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNASKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQAYTLTLTAVDTDGNPVTGEASRLR
                     FVPQDTNGVTIGTISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTVKDAYDNPVTSLTPEAPSLAGAAAVGSTASGW
                     TNNGDGTWTAQITLGSTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLIAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAASTGTERPSTGDWTETSNGVYVATLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIRDMTVKVDNQLANGQSTNQVTLTVVDTYGNPL
                     QGQNVTLTLPKGVTSKTGNTVTTDAAGKADIELMSTVAGEHSITASVNNAQKTVTVKF
                     KADFSTGQASLEVDSAAPKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANGEGKAELQVVSVTAGTYEITASAGNSQPSDTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAATYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
BASE COUNT         3699 a         3584 c         3106 g         3822 t
ORIGIN      
        1 ctgtatatca ataaagataa tataaaagtg agttacagcc taaaaataaa ccagacaaca
       61 ccagacatac ttgttaactg tgatagcaat aataactttg cctgtcagac cgcaatgact
      121 aaagatgtta tacagcgaat tactacattt tttcacacca gcccagatgt caagaacaga
      181 gaaataaagc tggaatggtc atgagataag agagatttac caactgctga atcggaaata
      241 tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag aataccataa tggcaggcaa
      301 gttctcgatg agatacaaat accttcagca attaacagtg agctttatac aaaaatgata
      361 tacctgacca ggaactggtc attgtatccg aacggtgatg gctgtgtaac tataagctca
      421 ccagaaataa aaaataaata ccctgctgcc atttgcctgg ctctgggatt ttttctaagc
      481 attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg tagatgaata ccaacaaaac
      541 tctgggcagt aataaacaac attctgatag agagtgcctg tataaagctg aagtgacaat
      601 gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc tttttcactg taaatatcct
      661 ggcattacta atgccaggat atttacagaa gagcagcata accatgacgg aacggggtta
      721 agtcaggttg tccttaacgc tattttcaac ctcgtatgcc ttcttcaggt ttatgttcag
      781 atctcatatc tctctcagca atcctcaata atcagataca ccgctttcac cgggccatga
      841 accccgacga ctttgataag ctcaatatcc gccgttgaac tagggccgct aatgatgtta
      901 atgcaggacg gcattcgttc accggcctgc gctttctgat gcaatttttc tgcgagttgc
      961 gctacacgcg gcaggattgt gcttttacgc aggataaaaa gagaagattc cgggagcagg
     1021 ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca cgcctcccga ttcggttaaa
     1081 ccatattcag catacacgac acccacttta gcctgctctg cctgcgagat attctcggca
     1141 cctttcgccg gatcccaaac aacggcattg cattcctgct gcaaacgttc gctaatcccc
     1201 aattcctcta gcctcgtgtc accgctaatc acgaccgact gatctcccag ctctttacac
     1261 agacgtattg cagcttctgc cgccttcgcc tcgctggtca gctcacagcg cgtcaacata
     1321 acatcgctgg caaactgaat aaacgcgtca cagcgctgct gttggttaag tgcggtaagc
     1381 cgctcgttag catagttgtt aagcggcgca tcttctgctt gcggttcaag tcgcaacggg
     1441 cgacccagtg cctgagcaac gttattcaaa aattcgctcc gattatccat tctttttctc
     1501 ctgcgccaga tgtttcttaa accaactacg gaaactctct ccgttagctt caggaagatc
     1561 gcgtgcttcc atccagtcac taatcgcgcc aaatttgagt ggtgttttgc cgccattgat
     1621 aaaccagctt gccgcatgag caccggccat catcccgact ttccacaatc ctggatgact
     1681 attggcataa gcgaacattt ttatcgcccg ttgctctgct tttgcggtga tccctttttc
     1741 agccatcacc cgacgatgac gcaaaatcag ttttgacagc ggaatacgca ccggacacac
     1801 gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa tctttaaaat ctttatagcc
     1861 gccaagtagc ggagaaatca ccgcaccaat tggccctgga taaatagagc catatccatg
     1921 accgccaata tggcgatatg ccggacaagt attcatacaa gccccgcagc gaatacagcg
     1981 cagcacatcc cgaaattcag aggccagcac ctcagaacgc ccgttatcga caataaccag
     2041 atgaaactct tcaggaccat caacgtgccc cgcttcgcgc ggtcctgtca gccaggtgtt
     2101 gtatcccgtc aaacgtgcac caacggcact gcgcgccagc atggtgatca atacatctac
     2161 ctcggcaaac gtgggggcaa tacgctccat tcccatcact gcaatatgcg ttttaggcag
     2221 cgtggtacac attcgcgcat taccttcatt ggtcaccagg cataccgaac ctgtctctgc
     2281 caccgcgaaa ttacagccgg taatacctat ttcagcactg aggaaatctt cgcggatttt
     2341 ttgccggatg aataaggtca tcgcttcagg cgtttccggc ccctcatagc ccagacgttc
     2401 gtgtagcact cgacggatct gatggcgatc tttatgaatt gccgggacca caacatgaga
     2461 tggcggatct tgatccagct ggagaatata ttcacccaga tcggtttcaa tcacctgaat
     2521 gccagcatcc tgcaacacat gattgacacc aatctcttcg gtcaccatcg atttagattt
     2581 caccaccttc cgggcatttt tgcgttgggc aacctgtaaa atgtagcggg tagcgtcttc
     2641 tttggttttt gcaaaccaga cgtgaccgcc gttttgcgtc actttttctg agagctggta
     2701 cagataagcg tcgagattac tcagaacatg atcacgtatc tgggcggccc gatcgcgcca
     2761 ctcctcccag tgccccaatt catcgaccat tttttgccga tttgccccaa tacgctgctg
     2821 cgcgtttgcc accgctttgc gcatgatcgg atcttcaatt tgctgacgga tgcgtgtctt
     2881 aaaatctgta ttactggttt tgatcgacat ctttatatcc tcagcggctc atcaacactt
     2941 cagcaatatg catcactttg actttctgcc cttcccgttg taatcgccca ctgatgttca
     3001 gcaggcaact cacgtcagca ccaattaaat actcagggcg gacttccatc aggtgcacaa
     3061 ccttttcttt caccatctcg ccggatattt cggccatttt gaccgagaac gtgccgccaa
     3121 atccgcagca ggtatcctgt tcagcaaagg tcaacagctc cagtccacgc acatttttca
     3181 gcagcgtaag tggctcgtcc ttcactccca gcttacgggc caggctacaa gatgggtgat
     3241 atactgctct cccttgcaaa ctggcaccta catcgactac ccctaattta ttaacaataa
     3301 aagaggtgag atcctgcata cgcgcggcaa ccttttcggc acgtgatgcc cattcaggtt
     3361 catccgccag atacgtcggg taacttttta cggcataggt gcaagagcca gccggtgaaa
     3421 taatgggatc gtcgttatcc tccagtgcgg cgatcagatt tttcatccct ggaatcgctt
     3481 ctttgatata accgctattg atcgcaggct gaccgcagca tccctgtttc tccgggaaat
     3541 ttacgcgaca gccgagtttt tccagtagca gcacggagtc tcgtgccatt cttgatttca
     3601 gggcgtcacc aatacaggtg acaaagaaat tgacattcac aactaatact ccattacttc
     3661 atgcccattt atgcccgatg tttccccccc accgggggat caatcatcaa taaatatggc
     3721 gttctttttt aattggctag caacatacca cccctcattt gtatgacaaa tgataatctt
     3781 tcatccaaac ttcaaatgca ataactaatt ttatttcttt cggcatcgct cacattttat
     3841 ggggaaatcg acgttcattt ttggcataag aaatccattt aaaaaggcta catcccttat
     3901 acctattcag agctacttat tcaggggtta ttgagatata cactgatggt gtacaaaaaa
     3961 atcaccgaca ataatcacgt atcaagtaat agtcggggtt ttatttaatt gaagcaattt
     4021 agtttgcatt tttatttaaa aactgagcta gagtccatca gaaaaaaata tataaagccc
     4081 aaaccaccgt tggaaataga aatagttgaa aggctcacaa ctcccggaat gcggattatc
     4141 tttgccctga tttctggcgg ttttattcag ggtgcaagct gtctgaccct ttcccgatat
     4201 tctcccggag tacaaccaaa ctcgcggaca aacgccttgt gaaaagatga ttcactggca
     4261 tagcctactg actcagcgat caccacaaca gggagcattt cccgggaaaa catctgggcc
     4321 gctatttgta gacgcaactt tgttaataca gccagcggcg tggttccgga aacatcacgg
     4381 aaaagctggg caaaacttgc ccgggacatg tgagcgatgc tggccagcga ttcgacggtc
     4441 caggcgtgtc ctggcatttc cagcatttgc tgtattaccg caccaagacg tggatgcaga
     4501 agcaaactga gaatgttttt ctcagtatta acctgtgcaa tccactcacg caccgcgagg
     4561 gtaaagaatg tagcgcagat ctggctacac agtgcatcca ctcccggcat cgccgatctg
     4621 gattcctgtt gtaaaaacgg gatcgcctcc ctcagccagt tatattccac gctgtggtta
     4681 accggtgcta aaaacagcgt ttccggcaaa gacgttaaaa aataacgcgc cgaatgttgc
     4741 aaccgaagag tgccgcagac aatacaggtc gattcattat cgacatgact caggcgatga
     4801 gctgaatttt gtggtagcag gaccacattt cccgggcgta atgtaaaaat ctctcccgtc
     4861 ggcatttcca gcttcgccgc tccttgcgtt aacgcatgcc aacgaataac cgataattcc
     4921 ccggcaccat gtggaagctg ccagtcactt cctaacacgc aattcttatc gatcgttcct
     4981 tgtggagcgt taagcatcaa caaacggcta agggcatcca tatcagactc ctgagtggtt
     5041 tttgattaca aacaccaaca atcttagtaa ataactaatc taataccatc aaatggttaa
     5101 tcatgatttt tgttttgtga ttctgattaa gaaaaagaaa ataccagacg ataagacaaa
     5161 atatcaagcg ttgtgaagaa atggtatttg ctcttttgcg tctatagtca tgatgtcaaa
     5221 tgaacgcgtt tcgacaggaa atcatcatga ataaatatca ggcagttatt attggttttg
     5281 gcaaggctgg aaaaacatta gccgtcacgc tggcaaaagc aggttggcga gtggctctca
     5341 tcgaacaatc aaatgcaatg tatggcggga cctgtattaa tatcggctgc atcccaacca
     5401 aaacattggt tcatgacgca cagcagcaca cagattttgt ccgtgccata cagcgtaaaa
     5461 atgaagtggt taatttttta cgtaataaga attttcataa tcttgcggat atgcccaata
     5521 tcgacgtgat cgacggccag gcggagttta tcaataatca tagcctgcgt gttcatcggc
     5581 ctgagggaaa tctggaaatt catggcgaga aaatttttat taataccggt gcacaaaccg
     5641 tggttccgcc aattcctgga attaccacca cgcctggtgt atatgacagc accggattac
     5701 ttaatctaaa agaattgcct gggcatttag gtattttggg cggcggatat attggcgttg
     5761 agttcgcctc tatgttcgct aattttggca gcaaggtaac cattttagaa gcagcttcgc
     5821 tgtttttgcc tcgggaagat cgggatattg ctgataatat cgcgacgatt ttacgcgatc
     5881 agggcgtcga tattatcctc aatgcccatg tggagcgaat cagtcacaat gaaaatcaag
     5941 tgcaagtgca tagcgagcac gcccaactgg cggtggatgc actgttaata gcttccggtc
     6001 gtcaaccggc taccgcttcg ttacatccag aaaatgccgg tatcgcagta aacgagcgcg
     6061 gggcaattgt cgttgacaag caattacata ccaccgcaga caatatttgg gcgatgggag
     6121 atgttaccgg cgggctgcaa tttacttata tttcactgga tgattaccgc attgtgcgtg
     6181 atgagttgct gggagaaggc agacgtagta ctgatgatcg gaaaaatgtg ccttattccg
     6241 tattcatgac accgccactg tccagggttg gtatgacaga agaacaggcc agagagagtg
     6301 gtgctgatat tcaggtggtg acattgcctg tagctgcaat tccgcgtgcc agagtgatga
     6361 atgatactcg tggggtatta aaagcgattg ttgataataa aacccaacgt atattagggg
     6421 catcactgct gtgtgttgac tcccacgaga tgattaatat agtgaaaatg gtgatggatg
     6481 ccgggctgcc ttatagcata ttacacgatc agatatttac tcatccgtcg atgagcgaat
     6541 cactcaatga tctattttca ttagtcaaat aaactcaaaa tcagacgcca gaacaaatat
     6601 tctggcgtct cagagaaaag aatcttatta attccctgtt actctataac ttcttaatca
     6661 cttcattgat ggtattttat atgttcaaaa aatctgtttt atttgcaaca ctattatctg
     6721 gcgttatggc attttccacc aatgcagatg ataaaataat tctgaaacat atcagcgtct
     6781 cgtcagtatc agcatcaccg acagttctgg aggataccat tgctgatata gccagaaaat
     6841 ataatgcttc atcctggaaa gtcacatcga tgcgaattga taataattca accgcaacag
     6901 cagtattgta taaataagga tgttcacaat ggaaaaatac ctgcacctgt taagtcgggg
     6961 agataaaatt ggcctgacat tgattcgtct gagtattgca attgttttta tgtggattgg
     7021 gttattaaag tttgtccctt acgaggcaga cagcattaca ccattcgtcg caaacagtcc
     7081 actaatgtcg ttcttttatg aacacccgga agactataaa cagtatctga ctcacgaagg
     7141 cgaatacaaa ccagaagcaa gggcatggca aacggccaat aatacctatg gtttttccaa
     7201 cggtcttggc gtcgtggagg tgattattgc tctgctggtt ttggctaatc ctgtcaatcg
     7261 ctggttaggt ttattgggag gactgatggc atttaccacg ccgctggtaa cactctcatt
     7321 tttaatcacc accccggagg catgggtacc cgcattgggt gacgctcatc atggtttccc
     7381 ttatttatcc ggtgctggtc gcctggtatt gaaagatact ctgatgctgg caggtgctgt
     7441 catgataatg gcagattcgg cgcgggaaat tcttaaacaa cgcagtaatg aatccagttc
     7501 aacgttaaaa actgaatatt gataatccga acactgtctg ttgttcactt tttcaggcgt
     7561 caggccccct ttctgatgat gaagcctgac gccatattac cggagatatg acagagtgaa
     7621 gatcaatatc actcccctgt taaaagattc ttgtcgtctc aggtaaaata tatgattaat
     7681 atcaatcatg tatttttaca gcaagacaac cacgaacaaa ttcaggatta aaatgtttta
     7741 ccgactcccc aacataaccc aaatcaagcg aactaatttg tgccatttct ttctctgtca
     7801 gtgagaaatc ccagatagca aaattttctt cgatacgctg ttgatgtgtc gatttcggaa
     7861 taacgaccac tccccgttga atattccaac gcagaatgac ttgagaaatc gatttttgat
     7921 gggcatctgc aatactctga agcagattat tttcaaaggg tttatgtcgt ccaccaccta
     7981 acggagccca tgcttcaggc tgcacgttat aatacttcat ggttgccagc gcctctggtt
     8041 gggcaaaata gggatgcaac tcgacctggt tgaccatcgg tttaactctt accgtttcac
     8101 aaaatttcgc cagaacatga ggatagaaat tggaaacccc aattgctttt aatttgccct
     8161 cttcataggc atcttcgagt gcacgccacg cactgaaata atcgcgcata gcctggtgca
     8221 ataaatagag gtcgaagtac tctagtcccg atttttttaa tgatgcttca atacctgctg
     8281 ctgccgtatc ctgattcaac atatcctgca cccacaattt agacgttata aataactcat
     8341 ctcgggtaca taaaccttca gatatagctt cacgaacggc ttcaccgacg gcatcttcat
     8401 taccgtatac cgctgcagtg tcgatgagtc gataacctgt gcggatagcg ttcaacactg
     8461 actgtttgca cacgttttta tcggtaacct gaaaaacacc aaatcccatc atcggcattt
     8521 tcagattgtt acttaatacg gaaaattcca ctgtcagtcc ctccattagg catgaacaat
     8581 gagtctacgt taaaacgtaa cctcaaatta gtacgtggat tttgatatca cttatgcaaa
     8641 aaattcatta ataatgcagg actgaaacct ctctattttc ggggacaacg aagcagacgc
     8701 taccagtgct tttgccttcg cccttgctgt ttttgataca cttagggccc agggcataac
     8761 gaaaatgtgc gatatgacga agaatcaatg gagaatgtga tgatcaggca gaagattcta
     8821 cagcagctcc tggagtggat tgagtgcaat cttgagcacc ctatttcaat cgaagatatc
     8881 gcacagaaat ctggctacag cagacgcaac atccagcttc tgttccgaaa tttcatgcat
     8941 gtgcctttgg gagaatacat tcgcaaacga aggctttgtc gtgccgccat tcttgtccgg
     9001 ctcaccgcga aatctatgct tgatattgca ctctctttgc attttgattc acagcagtca
     9061 ttcagccgcg aatttaaaaa gttattcggc tgctctcccc gtgtataccg ccaccgtgat
     9121 tattgggatc tcgcaaatat cttcccttct tttttaatac gtcaacagca aaagaccgag
     9181 tgtagattag tcaactttcc tgagacacct atttttggca attcatttaa atatgatatt
     9241 gaagtgtcga ataaatcacc ggatgaagaa gtcaaactgc gacgtcatca tttagccaga
     9301 tgtatgaata attttaagac ggatatctat ttcgtctcca cgtttgaacc gtcaacaaaa
     9361 tcggtcgatt tgctcacggt tgaaactttt gccggtacgg tatgcaaaca aaccgattca
     9421 gagatgccga aagagtggac aattaaccga ggactgtatg cctctttccg ctatgaaggt
     9481 gagtgggagc actaccctga atgggcgcga aacctctatc tgatggaatt acccgccagg
     9541 gggttagcca gagtgaacgg cagtgatatt gagcggtttt attacaatga aaatttcgtc
     9601 gaaattgata gcaataacat tgtttgcgaa atttttatcc ccgttcgtcc ggtttagttt
     9661 tcgctatatc taaatgaatt tataacgaga ctactgtcag gaaattcaat aaataatggc
     9721 gttgaagatg tcaacgccat tattgtttat tagaatgtta ctttcatatt cttaaaatta
     9781 tttcatcgcc tcctcgtcct ttgccgtatc ggcatccacc gcggtaatgg tcaccgtcac
     9841 tggcttcatt tcgtacccgc cataggtcag ggcgttaaat gtgaacgtat agacaccagg
     9901 cttgctggta ctaaatttac gcgtgatttt tccgctcacg aaatggtcat ttttcgacgg
     9961 caggaactga taatcctttt ccgtcacccc ttccggcgtc tgaatgtcga cccacatatt
    10021 cccgccaacg gggttgtttg ccgacatcag tgtcacctcc agcttcgcag tcgatttccc
    10081 atccgccacc agagattgca tatcagatga tgcggtgagt accgcgtttt tatcatccgc
    10141 aacgaattta gattcggcag ttttcgttga tacctgaccg ttggtctgac tcacttgcgc
    10201 cttgagtgtg tatgtcgccg caacagtagt ggtagcggtg aaaatagcct gcccttgctc
    10261 attcgttgtc gcagtgccat tcggttccag gtttaacgaa gccgggctgg cagtcagcgt
    10321 cacttcgcta tctttcacca aattgttatt ggcatcagtc accgtaactt tatacgtttg
    10381 tttggctttg ccgtccgcca gcgcatagtt gcccatcacc tcaataccgg agacggttgc
    10441 ggtagcctta tcggctacaa acgttatagt ctgcgtatcc gaaggctggc tattccccgc
    10501 cgatgccgtg atctcatacg ttccggcagt cactgaaacc acctgcaact ctgctttccc
    10561 ctcaccgttg gctttcaccc agacattatc gcctgtaagc ggcttgacac cccggggcag
    10621 attaaaggtc accaggctcc ctggaacagg gttaccattt ttatcctcaa cggtcgccgt
    10681 cagcgtaaag gcatctttgc cgtttgccac ttttggcgcg gcgctatcaa cctccagact
    10741 cgcctgaccg gtactgaaat ccgccttgaa tttcaccgta accgtcttct gagcattatt
    10801 cactgaggcc gtgatgctgt gttccccggc aaccgttgac atcagctcaa tgtcggcttt
    10861 acctgccgca tcggttgtta ccgtattccc cgtcttgctg gtcacacctt tcggcagagt
    10921 cagcgtcaca ttttgtccct gcaacgggtt accataggtg tccacaacgg tcagggttac
    10981 ctggttagtc gattgtccat tagccagctg gttatcaacc ttcaccgtca tatcacgaat
    11041 ctcagcctta gatgcgtcac cagcaacatt cagcaccagt ggctgagcaa cggcattttg
    11101 gccgttcact cgcggcatca cagacaactg gcccgcggca gatcccagag tcaaggtcgc
    11161 cacgtagacc ccattacttg tttctgtcca gtctcctgtt gaaggtcgct ctgtccccgt
    11221 gctggcggca ccgctaaaca ctggtgcatc tggcttcatc ccggtgaccg ggttcccgta
    11281 cgcatccttc acggtgaagg tgagttccgt cgtcgttttg acggtcggag ccttattgtc
    11341 cgcaacaagc gtagagttcg ctgatgacat ctctccggcg atgaccgtca actgcgcggc
    11401 ttcggtggct gctggctggc cgttgaatag cggcatgaca cgaagctcgc ccgtctttcc
    11461 gcctgtagtt aacgtagcaa cataggaacc gtcacctttt tcggtccaac tggaaacggt
    11521 cgccccttca gaggcggtcc ccgtcaaact tgccgacaac gaaagaccac tgatagcgtt
    11581 gccatgtgca tcttttgcaa taagcgtcac ggttgtactt tcgccggctt ttacgtgatc
    11641 ttctgcgaca gagactttcg actggtttga agataacgca tcagccacca cggttacttt
    11701 tgccgcattt gctgccgcat cctgtccatt tagcttcggc ataacttcta attcacccgc
    11761 cgtagagccg agagtaatct gcgcagtcca cgtcccatca ccattatttg tccagcccga
    11821 tgccgtagaa cctacagcag cggcacccgc taatgacggc gcttccggcg tgaggctggt
    11881 cacagggttg tcataggcat cttttaccgt ccagattgcc gtaactgtac ccccaaccac
    11941 cggtttatca ggattcaggg tgatggacga atgtgctgca tcaagcggcc cggcaacaaa
    12001 cttcagcgtt tgttgtaatg tgcccagctg atactgctcg ctgaaagcac gcacaacaac
    12061 gtttccggca cgggtcgaag aaaccgtagc gctgtaaacg cctggtttta tctccgaaat
    12121 tgtaccaatg gtgacaccat tagtgtcttg cggaacaaat cgcaagcggc tggcctctcc
    12181 cgtcaccggg ttaccatcag tatccaccgc agtcagcgtc agcgtgtagg cttgctgacc
    12241 atcagcaacc acgtcacctg acggctcgtt agcgctcagg gtggagtttg ccacatccat
    12301 catcgtggcc cgcagttctg cagtgacggt tttgctcatg ccatcaacac taacagtaat
    12361 cgttgcctca cctgactgcg ttccggtagt aaagacagac tgatacaccc ctgcttcagt
    12421 ttcggtgaac tcacccagtg ttggctttgc ctgtgattta gtggccttca gggtacgagt
    12481 cacaatattt ccagccggtt tgaaagttag ttcagtcttg atctgatctt tcatgcccgt
    12541 gactggctgg ccctcggcgt cgcgcagaga cagcaccagc ggtctttgct cattaccgtt
    12601 agcaagcatt tgaatacggc tctgaccgtc aagcgttaac gccgtgcgat cggcactcat
    12661 acctgctccg gtaatgacca cctctgtctg cacgcgtttt gaggcattgc ctttgttatc
    12721 gtaggcaacc gcagaaatcg cataataatt gtctttgcct ggacgataag ccgggagcgt
    12781 tacttgccac tgactaccct gaccggtaat tttgccacct tcagccagta atgacggcgc
    12841 ttcccactgc acatttttca gtccgtgagt tgctttgctg accacaagcc ccagggaaag
    12901 tgtctgaccg cccttacctt caatacgctc aggcagagca atacggatca cttcagattt
    12961 gcggtactca agaacgatgt tgttattacg ctcaaccagg tcatagcggc tgcctgccag
    13021 cattcgacgc tcgcgaatgc tgtctgtatc gagttgtttc tccagaggtt cgccaatccg
    13081 ataattaact tccaggccaa agcgagtgtc attctcaccg ctcttgccct gcttatgccc
    13141 ggcgctcagt gtcagaagag gcactggcgt ataggtcacc tcggcagaaa tagcatgcgg
    13201 gtctttctgg cgcttatctt taccaaacag cccgacttca tcgccataat actgttcata
    13261 catcaggctt gcgccaagct gcggccaggc aggtaaatag ccctctgcgc ggatatccca
    13321 accattcgcc gggcgttcct gataatcctc aatatccggc gattttttcc agccagaagc
    13381 ccgaatataa ccattggcgc tcagtttcag ataatcgcgc cagtattccg caccaacacc
    13441 aatgcgggta tgactacggg ataaatcatg atcgataaaa gtattcaccc ccgccatcca
    13501 gtcatttccc gaaaaatgac gccagccaaa accaatattt gactgagtac gatcgtctgt
    13561 acgatgtatt gccccctgag tgaacaacat atttgtcggc gtatcataaa tcggataaag
    13621 catttccagc gaagaatcct tcagcgagaa atctttatcg acattcagtt tgacgcgcgc
    13681 tgtaccatat ttcccgagcc actcctgtat ttcctggtta gctttagcgg tggccattcc
    13741 ggtaataaaa ttacgtgtcg catcgctatc tggctgactg cttaaaaatg tcccggcatt
    13801 tgcggcaaac gacgcgacat ttttctccac attattatca gcagttaccg tggtattttc
    13861 catgctcaac cgtggctgaa ccgcatgctg cgcatgtgcc gccattactg gggtaaaggt
    13921 gacagcgagt ggaaaaagaa cctgaacaga gatatttgcc cacgccacgc agcgggccag
    13981 aactgaataa cgaaatagtg gttgtttatg acctgtttta taacgtgaca ttgagataat
    14041 ttcctgatga acgaattaac gaacataggc agacacaaat aatggcgtct gcttgtcaga
    14101 aaaaccggag atttcccggt ttccttttcc gcagaaaagg aaataccttt atgtaagaaa
    14161 gtacaccgcc acctaatata tccagtgcct gtagccactc atcaggtggt t
//
LOCUS       DAZFXT010000118        14041 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.118, whole genome shotgun sequence.
ACCESSION   DAZFXT010000118 DAZFXT010000000
VERSION     DAZFXT010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14041
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.118"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..916
                     /gene="hlyE"
                     /locus_tag="ACHKW0_003801"
                     /pseudo
     CDS             6..916
                     /gene="hlyE"
                     /locus_tag="ACHKW0_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            complement(1123..1569)
                     /gene="ycgN"
                     /locus_tag="ACHKW0_003802"
     CDS             complement(1123..1569)
                     /gene="ycgN"
                     /locus_tag="ACHKW0_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HFO7476558.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(1661..2320)
                     /gene="ycgM"
                     /locus_tag="ACHKW0_003803"
     CDS             complement(1661..2320)
                     /gene="ycgM"
                     /locus_tag="ACHKW0_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HFO7476559.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            complement(2392..2685)
                     /locus_tag="ACHKW0_003804"
     CDS             complement(2392..2685)
                     /locus_tag="ACHKW0_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HFO7476560.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            2927..3328
                     /gene="pliG"
                     /locus_tag="ACHKW0_003805"
     CDS             2927..3328
                     /gene="pliG"
                     /locus_tag="ACHKW0_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="g-type lysoyme inhibitor PliG"
                     /protein_id="HFO7476561.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            complement(3448..3816)
                     /gene="ycgJ"
                     /locus_tag="ACHKW0_003806"
     CDS             complement(3448..3816)
                     /gene="ycgJ"
                     /locus_tag="ACHKW0_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HFO7476562.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            4336..5031
                     /gene="minC"
                     /locus_tag="ACHKW0_003807"
     CDS             4336..5031
                     /gene="minC"
                     /locus_tag="ACHKW0_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA];
                     GO_process: GO:1901891 - regulation of cell septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HFO7476563.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            5055..5867
                     /gene="minD"
                     /locus_tag="ACHKW0_003808"
     CDS             5055..5867
                     /gene="minD"
                     /locus_tag="ACHKW0_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HFO7476564.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            5871..6137
                     /gene="minE"
                     /locus_tag="ACHKW0_003809"
     CDS             5871..6137
                     /gene="minE"
                     /locus_tag="ACHKW0_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0032955 - regulation of division septum
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HFO7476565.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(6509..6853)
                     /locus_tag="ACHKW0_003810"
                     /pseudo
     CDS             complement(6509..6853)
                     /locus_tag="ACHKW0_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(6888..7007)
                     /locus_tag="ACHKW0_003811"
     CDS             complement(6888..7007)
                     /locus_tag="ACHKW0_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HFO7476566.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(6968..7153)
                     /gene="ymgJ"
                     /locus_tag="ACHKW0_003812"
     CDS             complement(6968..7153)
                     /gene="ymgJ"
                     /locus_tag="ACHKW0_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgJ"
                     /protein_id="HFO7476567.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            7254..7427
                     /gene="ymgI"
                     /locus_tag="ACHKW0_003813"
     CDS             7254..7427
                     /gene="ymgI"
                     /locus_tag="ACHKW0_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgI"
                     /protein_id="HFO7476568.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            7429..7773
                     /gene="ymgG"
                     /locus_tag="ACHKW0_003814"
     CDS             7429..7773
                     /gene="ymgG"
                     /locus_tag="ACHKW0_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HFO7476569.1"
                     /translation="MKKKILTFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            7777..8112
                     /gene="ymgD"
                     /locus_tag="ACHKW0_003815"
     CDS             7777..8112
                     /gene="ymgD"
                     /locus_tag="ACHKW0_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HFO7476570.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPDLSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(8169..10490)
                     /locus_tag="ACHKW0_003816"
     CDS             complement(8169..10490)
                     /locus_tag="ACHKW0_003816"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7476571.1"
                     /translation="MQGKNTIVITGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGADLLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYATWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(10607..10816)
                     /locus_tag="ACHKW0_003817"
     CDS             complement(10607..10816)
                     /locus_tag="ACHKW0_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476572.1"
                     /translation="MKLKKLSGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            complement(11216..11434)
                     /gene="ymgF"
                     /locus_tag="ACHKW0_003818"
     CDS             complement(11216..11434)
                     /gene="ymgF"
                     /locus_tag="ACHKW0_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division-associated protein YmgF"
                     /protein_id="HFO7476573.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVNHRDQQ"
     gene            complement(11566..13089)
                     /gene="pdeG"
                     /locus_tag="ACHKW0_003819"
     CDS             complement(11566..13089)
                     /gene="pdeG"
                     /locus_tag="ACHKW0_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7476574.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(13421..13669)
                     /gene="ymgC"
                     /locus_tag="ACHKW0_003820"
     CDS             complement(13421..13669)
                     /gene="ymgC"
                     /locus_tag="ACHKW0_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmgC"
                     /protein_id="HFO7476575.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
     gene            complement(13782..>14041)
                     /gene="ariR"
                     /locus_tag="ACHKW0_003821"
                     /gene_synonym="ymgB"
     CDS             complement(13782..>14041)
                     /gene="ariR"
                     /locus_tag="ACHKW0_003821"
                     /gene_synonym="ymgB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm/acid-resistance regulator AriR"
                     /protein_id="HFO7476576.1"
                     /translation="DTTIHNAITDKALASYFRSSGNLLEEESAVLGQAVTNLMLSGDN
                     VNNKNIILSLIHSLETTSDILKADVIRKTLEIVLRYTADDM"
BASE COUNT         4020 a         3243 c         3009 g         3769 t
ORIGIN      
        1 tgattatgac tgaaatcgtt gcagataaaa cggtagaagt agttaaaaac gcaatcgaaa
       61 ctgcagatgg agcattagat ctttataata aatatctcga tcaggtcatc ccctggcaga
      121 cctttgatga aaccataaaa gagttaagtc gctttaaaca ggagtattca caggcagcct
      181 ccgttttagt cggcgatatt aaaaccttac ttatggatag ccaggataag tattttgaag
      241 caacccaaac agtgtatgaa tggtgtggtg ttgcgacgca attgctcgca gcgtatattt
      301 ttctatttga tgagtacaat gagaagaaag catccgccca gaaagacatt ctcattaagg
      361 tactggatga cggcatcacg aagctgaatg aagcgcaaaa atccctgctg gtaagctcac
      421 aaagtttcaa caacgcttcc gggaaactgc tggcgttaga tagccagtta accaatgatt
      481 tttcagaaaa aagcagcttt tccagtcaca ggtagataaa atcaggaagg aagcgtatgc
      541 cggtgccgca gccggtgtcg tcgccggtcc atttggatta atcatttcct attctattgc
      601 tgcgggcgta gttgaaggga aactgattcc agaattgaag aacaagttaa aatctgtgca
      661 gaatttcttt accaccctgt ctaacacggt taaacaagcg aataaagata tcgatgccgc
      721 caaattgaaa ttaaccaccg aaatagccgc catcggggag ataaaaacgg aaactgaaac
      781 aaccagattc tatgttgatt atgatgattt aatgctttct ttgctaaaag aagcggcaaa
      841 aaaaatgatt aacacctgta atgagtatca gaaaagacac ggtaaaaaga cactctttga
      901 ggtacctgaa gtctgataag cgattattct ctccatgtac tcaaggtata aggtttatca
      961 catttaaaaa cacagacgtt ttttaaagat tgagagttac ctgttgataa taaggtatcg
     1021 ggtaactctc attatgacag agcgctttca aaaacagggc tacgaaatgg cgagcatcca
     1081 gacgtatatt aagaaagccc ccatcaacat ctattaaatt tatcactgcg cccagtcagg
     1141 tttatttaag atatgatcct gccagtcaat cacttctgat tctttcactg cgatatgacg
     1201 cacagagata cgttcaccat gcattgccgc tttcgaacca gtaagtagcg gatgccacgc
     1261 aggtaaatct ttaccttccg ccagcaaacg ataagcgcag gtcattggca gccattcgaa
     1321 tgttggcaga ttttcacggg ttaatttaat gcagtcgggt tcaaactcga aacgacgttc
     1381 gtagttccga cattgacaag ttttaatatt gagctggcga caggcgacgt tagtgaagta
     1441 gatttcgtcg gtgtcttcat ccatcagttt atgcaggcaa cactgaccgc aaccatcaca
     1501 caacgactcc cattccgcat cgctcatttc gtccagggtt ttactttgcc agaaaggtac
     1561 atcgctcatc aggtgttccg ccattacgtt aaaacgcacc ttataaccag tctggcacag
     1621 cgatgcaagt tttgccgccg ctttcaggcg gcaaaaagta ttacaaaacg cgagttgtca
     1681 aagaatgccc atcgaaagtg actgtcagct catcaccgct ttgcaacggg ccgacgccat
     1741 caggcgtgcc tgtcaacaca acgtcaccgg ccttgagggt aaaaaacttg ctcatataag
     1801 cgatcaacgg aacgatttta tggatcatgt ccgcagtcgt accttgctgg cgttgttcgc
     1861 cgtttacgct caggcccagc gttgtatttt gtggatcgcc ggtaaattcc gccgcgggga
     1921 taaacccgga aagcggacaa gagttatcaa acgctttagc cttttcccac ggctgcccgg
     1981 ctttcttcat ttttccctga acatcacgca atgtcagatc aagcgccacg ccgtaaccgg
     2041 caatggcttt gcggacatgc tcttccgttg cctgacgcag cgtcgcgcca atcaacaccg
     2101 ccagttcgac ttcatgatga actgaaccga aatcggatgg gatcgccaat ggctgccgca
     2161 gatcgcacag tgccgtttct ggtttaataa acagcactgg ctcttcgggc actgcgctgc
     2221 ccatctcttt aatatgtttg gcatagttac tgccaacaca gactacttta ctcaccggat
     2281 aatccagcag cgcaccttgc cagttgtgat gttgatacat aacgttcccc tcaccttatc
     2341 gtggatatga aaacccgcgc cgacaaagat aatgccaccg ccggatatcg gttatttgtt
     2401 agtgtcgtct gttttctgcc ccatgacgga aagatgttgc ttcagcaaat cttcgggtgg
     2461 tggcggtaac tgcaaatagt aaccttgctc ggttaatgcc agtttaacct tttcaatatc
     2521 agcattgacc agtttcttgc gcccatccag cggcagaatc atcgctaact gaggctgacc
     2581 aaaacctttc atcagttcct caggaacacg cgaaaaatcg tctttttttt cgacatataa
     2641 ataggtctgg tcacgcttgc tgcttcgata aatcacacaa aacatacttt tactctttaa
     2701 gatgccaggt tttggcataa gctaacatca atgaaacccg cgccacaagc cagccagctg
     2761 tagatttctg cggtttgttg agtccatgcc cctttataag taatcccccc ggcaaaaaat
     2821 gccgcgccgg gattttaccg aacgcagaaa agccactgga attctcccgt aagggattgt
     2881 tatattataa cagttcatcg tactcattct gaacaggaga ctaccaatga aaatcaagag
     2941 catcagtaag gctgtattgc tgcttgcact gctaacctcc acgagctttg ctgcgggtaa
     3001 gaatgtcaat gttgagttca gcaaaggcca tagcagcgct cagtactccg gcgaaataaa
     3061 gggatacgat tacgatacat ataccttcta tgccaaaaaa ggtcagaaag tgcatgtgag
     3121 tatctctaat gaaggcgctg atacttacct cttcgggcca ggaatcgatg attcagttga
     3181 tttatccaga tattcaccag aactcgatag tcacggacaa tactcactcc ctgcttctgg
     3241 caaatatgag ctgcgagtgc tgcaaacccg aaacgacgcc cgtaaaaaca aaactaaaaa
     3301 atataacgtc gatattcaaa ttaagtaaat gtcaaccgac ggggactctc cccgtcataa
     3361 acgttaacgc cccactcctc tctgtcgctc aagaggttaa tgacgaatag cagacaatgc
     3421 cgaatagaac ataatgtaaa caaggcatca ctcacgacac ataaataaca tctttgtggt
     3481 gatttgattg atttttccac tccgcttacc atccacacca aaataacgat catcacgaca
     3541 taatttttct ttaacatcgc aaaatacacc gttagcgaat gtaaattcgc tgggatcgta
     3601 gccttgtaat gattgtaagt tttcagcggc tttttcgccg agatatttcc ttgttaactc
     3661 tggggaaatg ccatctgagt tcacacaaat atacgggtcg caaacaacgc cgcacgccgg
     3721 agattgaagt ccgccagcgt catgtccaga cgccattgat gagaacatca tccccacacc
     3781 tgacaatccg atataaaaaa tacgcatcat attcatagca catcctgata atccaaccgt
     3841 acaacactta tgctcatttc atcatcaatc cgtaatcaaa accgctgacc tggacgagtt
     3901 acgatgacct gacagaagta tcagacgtct tctgcggttt ctatgataga gcaattctgt
     3961 ggcggatatt aagataacgg gtatctgttc tttaatgatg ccgcaaaaaa tggaaagtta
     4021 agaaagcctg gcgtggtgaa aaaatacact tgcctcaata ctggatattt aaacaggtct
     4081 ggagatgctg tcacgattag ggtgcggaaa aaggaagcgt aaataagaac aatcaaaagt
     4141 acgacggcaa tgggttgatt gacaagggta ttttttaagc tatgaatcag cgccatttat
     4201 cacagaatag acttttactc tgaataaatg ggagggtgac ttgcctcaat ataatccaga
     4261 ctataacatg ccttatagtc ttcggaacat catcgcgcgc tggcgatgat taatagctaa
     4321 ttgagtaagg ccaggatgtc aaacacgcca atcgagctta aaggcagtag cttcacttta
     4381 tctgtggttc atctgcatga ggcagaacct aaggttatcc atcaggcgct ggaagacaaa
     4441 atcgctcagg cccccgcatt tttaaaacat gcccccgttg tactcaacgt cagtgcactg
     4501 gaagacccgg taaactggtc agcgatgcat aaggcggttt cggcaaccgg tttgcgggtt
     4561 attggcgtaa gtggctgcaa agatgcgcaa cttaaagccg aaattgaaaa gatggggctg
     4621 cctatcctga cggaaggaaa ggaaaaagcg ccgcgtccag ctcccgcacc acaggctcca
     4681 gcgcaaaata caacgccggt cacaaaaacg cgtttaatag ataccccggt gcgttccggt
     4741 cagcgtattt atgctccaca atgtgatctg attgttacaa gccacgttag cgctggggcc
     4801 gaattgattg ccgatgggaa cattcatgtc tatggcatga tgcgcggtcg tgcgcttgca
     4861 ggggcaagtg gtgaccggga aacgcaaata ttttgtacga acctgatggc ggaactggtg
     4921 tccatcgcag gtgaatactg gctgagtgat caaatcccag cagaatttta tggcaaagcg
     4981 gcgcgactgc agttagtcga aaacgctttg accgttcaac cgttaaattg atcccttttt
     5041 aacaaggaat ttctatggca cgcattattg ttgttacttc gggcaaaggg ggtgttggta
     5101 agacaacctc cagcgcggcc atcgccactg gtttggccca gaagggaaag aaaactgtcg
     5161 tgatagattt tgatatcggc ctgcgtaatc tcgacctgat tatgggttgt gaacgccggg
     5221 tcgtttacga tttcgtcaac gtcattcagg gcgatgcaac gctaaatcag gcgttaatta
     5281 aagataagcg tactgaaaat ctctatattc ttccggcatc gcaaacacgc gataaagatg
     5341 ccctcacccg tgaaggggtc gccaaagttc ttgatgatct gaaagcgatg gattttgaat
     5401 ttatcgtttg tgactccccg gcagggattg aaaccggtgc gttaatggca ctctattttg
     5461 cagacgaagc cattattacc accaacccgg aagtctcctc agtacgcgac tctgaccgta
     5521 ttttaggcat tctggcgtcg aaatcacgcc gcgcagaaaa tggcgaagag cctattaaag
     5581 agcacctgct gttaacgcgc tataacccag gccgcgtaag cagaggtgac atgctgagca
     5641 tggaagatgt gctggagatc ctgcgcatca aactcgtcgg cgtgatccca gaggatcaat
     5701 cagtattgcg cgcctctaac cagggtgaac cggtcattct cgacattaac gccgatgcgg
     5761 gtaaagccta cgcagatacc gtagaacgtc tgttgggaga agaacgtcct ttccgcttca
     5821 ttgaagaaga gaagaaaggc ttcctcaaac gcttgttcgg aggataagtt atggcattac
     5881 tcgatttctt tctctcgcgg aagaaaaaca cagccaacat tgcaaaagaa cggctgcaga
     5941 ttattgttgc tgaacgccgt cgcagcgatg cagaaccgca ttatctgccg cagttgcgta
     6001 aagatattct tgaggtcatt tgtaaatacg tacaaattga tcctgagatg gtaaccgtac
     6061 agcttgagca aaaagatggc gatatttcta ttcttgagct gaacgtgacc ttaccggaag
     6121 cagaagagct gaaataagcc cgctgtaaaa acgcatttat cttcaaggca gagtcatttc
     6181 tgccttgagt ttttcatccc tctcatccac gttgtggtaa agcggcgagt attcttgctg
     6241 atactcctca ttgctatttc cacctccccc tttacacctt aaggctgttc ccacgcacag
     6301 aacaaaatgt ttatgcctgg ttgagtaaat aaccctgttg ttagtgtgga tatactggga
     6361 gatgataacc ccgcaatttc attattcatt gttctcccat aggatgaggt ttcccgccgt
     6421 aactggcggg ctttttttgc cccagaattt tctctttcaa catcctgtaa acgaaaactg
     6481 cgccgaagcg cagtttaatc gttgatggtc agaacgacca gttcacacca gccaccgcgt
     6541 tccacgggga ctccacaccg gcaccatggc tataccccac cccgaaatgc ccgcttaacg
     6601 tactgctgaa tgaggcttta atacctgcct ggtatattcc acgtctgccc gacaaatcat
     6661 tgacaaaatt accgtcacta ttcactttca cccgattatc atcgacaaat tctttgcgca
     6721 cagccgcctt caggcagggt tcaatttcca taccgttccc cagacgcatg ttgtaactca
     6781 gcgttgcacc cagttcacga tatatactgc gggtatcgac tgatttcgat tccatgccat
     6841 tggataaatg atagtagcgt ctaagtgacc taagctctgc tgcaacatca gcccgcaggc
     6901 accagaccag gggattcatc ctgaagagac agcgcaggtg tattgtgttc accgctcacc
     6961 aaagacatca tgatgaaatg atgatatccc gcataagaaa gaggcatttt ttacacgcag
     7021 tgcgctgaag tgtagttgga taaaaaatca ctcttttcag ataatactgt cgtacgctta
     7081 tctttcgaca atgaggccgt cggcaaaata aaatgactaa tgtaatcgtt gctgatgaat
     7141 atcttctgct cacataaaaa tcacacaata atattgataa atcagattat cttaccttta
     7201 caacattgcg ttaacagtca atgacacttc ttgaataaaa atattgtcaa tcaatgagtt
     7261 attcctcatt taagattatt ctcattcacg taaaaaacat aattcctatc atcacagcca
     7321 cgctcatttt gaattatctg aataatagcg agcgttcttt agttaagcag atcttaattg
     7381 aagacgaaat tattgtgtgt gcaacttact taataccgga catctaagtt gaagaaaaaa
     7441 atacttacct tcgggcttat ctcagcattg ttctgctcca cgccagcgat ggcggatatg
     7501 aatcgcacca cgaaaggtgc attgctaggc gcaggcgttg gtttacttac tggcaatggt
     7561 gttaatggcg tactgaaagg tgcagctgtt ggcgctggtg ttggtgcagt aacagaaaaa
     7621 ggccgcgacg gtaaaaatgc gcgtaaaggt gcaaaagtag gtgccgccgt tggtgctgtc
     7681 actggcgttc tgaccggcaa tggcctcgaa ggggcgatta aaggtgccgt gattggcggt
     7741 acgggtggtg caattctggg taaaatgaaa taaggaatga tgatgaaaaa atttgcttta
     7801 ctggcgggcc tgtttgtttt cgcaccaatg acctgggcgc aggactacaa tattaaaaac
     7861 ggtttgccgt cagaaaccta tattacttgc gcagaagcca acgaaatggc aaaaactgac
     7921 agcgcacagg ttgcagaaat tgttgcggta atgggtaatg ccagcgttgc cagccgtgat
     7981 ttaaaaattg agcaatctcc ggatctgagc gccaaagtgg tagaaaaact gaatcaggtt
     8041 tgcgctaaag acccgcaaat gctgttgatc acggcaattg atgacaccat gcgtgctatc
     8101 ggcaaaaaat aatatttcat atgtaccgcg tcgatgaata cgacgcggtt ttttagctaa
     8161 cggcgggatc agaacttata tttcaggccc accatcactg cggtgtcatt gtagccatta
     8221 tcacccagct gcacgcccac attcccccag acgctggccg ctggattcag gttgccattt
     8281 acgccaagtt taatttcccc caaattactc gccccatctt gttcgacttt cacaccgttc
     8341 attgagacag caaaatcttt actgttatgg atccagttca tttcaacaaa aggttcaaag
     8401 tcgtcaccgt gcggaccgct gtttttctcc tgagtacgaa taaaggtgcg gaaaccaagt
     8461 cgggtctgga tgttgttatt tgcgtcattc tcaatacgtg agccattggc ttcggtgtgc
     8521 gcattatgat cgacgcccat ccagatagcc tgtgcctgtg gctggaatat ccacgtgtat
     8581 ttcgccgctt ccagaccaaa ggatttattc agggcaaagc cgtaacccgc ttccagcgag
     8641 acggttgcgc ctttcgcact ataggattca taggctaatt catccccttt caccgagttt
     8701 ttaaaccagc tatattgcgc ccaggcgtca atgtatgcgc cttttttact gatgtcatcg
     8761 gcaaaccagg tggcatacag ccctgcgcaa tagcctctga cactcccttt cgaacgataa
     8821 tccgacacgc tggaatgagt tgagttgtag tcgcgggcat aaccagccat cactcccaaa
     8881 cgccaactgt cactgtcggt aaaaccaccg gttaacagat cggcccctaa ctgcgagacg
     8941 tagcgatgcg aggtcgttct caactgtccg ttgctgtcac gccaggcatt atgcccgcca
     9001 atttgacgta gccaaagtcg gcttgaacgc tcctgttcag ttacaggatc gatgtagcgc
     9061 atttcacccg cacggtcgtt cagacgcata acaaaaagag tgttagcggc tgccaggttg
     9121 gcgacgtaac ttccagcttc gaggcgcagg acgggggtag gcgcaacagt aggcgctgaa
     9181 cttcctccgg agtttgtttc accgccgctc gttgcctgga agttagtcag ataccagttt
     9241 ttattggtcg cttcggtatt acctcgggtt aatgaatatt cgtaggcacc ggcgaaaatc
     9301 ctcgaatcct taataaattc cccatttgat tccccctcaa cgctgataat ctctatccca
     9361 tttaacgtgt aagctccgct gcctcctgca ttggtaactt taactcgggt tgttccagag
     9421 gtatttccat taataacgac tttatccgta gctgaattat catcacccaa ttcagttctg
     9481 agatgcaata cgccattatt accaacgtaa ttttcagtta ttgttaatct tgttggttcg
     9541 acatcccgtc catctgcccg ggaaatatag accgtactat tatcaaccgt gaggttgctc
     9601 accgttgagt cagctttaat atcccaggta ctattatctg aaagcgacag ataggtatga
     9661 gtgttatcat ctgttgaaat attagctgaa cccgttattt ttgaattagt tgcatcgacg
     9721 gtcaaatgac ctttaatatt attgataaaa acatctgcag ccgatgatac ctcactatcc
     9781 gtaatggtca cgttactcgt ctcattttta tggtagagga tttcatttgc tgaccgtagt
     9841 gagctaccag ataaaatatc taccgttgct tcctgaccat ttatggaaga ctccagcaca
     9901 attccagacc cctgatgcgc gactgcagat gtatttttca gcgtcaccgt gccgccatta
     9961 taaattttag cagtcgcggc gtcaggaccc gttgtataaa tttctccctg gttaaatgaa
    10021 actgtcccct ggcgggcagc gatggcatga gcattgtccc catgcgtggt gatatcaaca
    10081 tcggttaatg taacgctggc attggtataa tcaagcgtgt caccagcctt gacgacagtg
    10141 ccattagcgt acgcggcata actttctgtt ccgtgggttg tgatctcccc ttttgccata
    10201 tccagttttg ctatggcatt attttcatta cttcctgtat cggtagtact ttcttcatca
    10261 agagaagata cacacgcacg cgcgcttccg ggagatgcac acgcaagaac gccgactgcg
    10321 tgattacccg cagtaacaat aaatgtatca tctccgtcag aaaaagaggg ggtaaccgaa
    10381 ccgtcagagt tgactcttat tatggtgtca gtattcaggt ttccactcgt ttgacttagc
    10441 aaaccaattg agtaatcccc cgttatgaca atcgtgtttt taccctgcaa aataacagca
    10501 ccgccaaatt cacttacgat tgcgctagct gttaatgctg tgcttagagg actattgtca
    10561 gaaggatata aaccgcccgt tgttacaatg acgccattgt taatggttaa cttgctaccc
    10621 tctcctgcaa cggatacccg taacttccat tacccgttgt tataactagc gcattatcaa
    10681 tatcttttac ttttccatct tttattgcca cagcatgttt atattcatcg tgctgaataa
    10741 actgttcagc atatgcatta cccaccgcca gagcaataag tcccaaacta aacccggaga
    10801 gttttttcag tttcatagat taaccatcca ttcattggat taacaaacat tcctggtcaa
    10861 ataacctatg cgttcagatt accaaaacgg agggtaaaga caaaaacaaa agcacaaaaa
    10921 atttaaatag ataaacagcg acaatatata aatgataaat aagtcggcat tatcattttt
    10981 caccttattt tcatgacatt gatcacttta gggtgacgat tttgtttaac aaataactct
    11041 tcgacatcaa aattgttaat agttgcgatg aggattattt cggtgatata taaaaacaac
    11101 cgtgataaca ccagggttga caactatttg cgcaataatg cctttccttc aatgatccag
    11161 gcgttcactg ccagcaaccc attcagccgc atactgacag tgagaaactg aaagattatt
    11221 gctgatccct gtggttgacg agtctttctc ttaatcgatt aaccagattg atcgccagcc
    11281 aaacaagggc attaagcaaa tttatcgtca caagcacttc tcccagcccg gtactgggtt
    11341 ttgcaataag gatgaccagg agggtgatca gcataaatgc aatggaaaag atgatataga
    11401 gagtgaaata atccagatta ttactattgt tcatgctact aatggcaatc ctgtcaaaac
    11461 tttcaaacaa ttatagcaaa caaaataatg aacaatagta acaagaggca acgagctaat
    11521 tagctgtcta taattacacc gcactatggc cttactgatc aattttcact caaccacaac
    11581 cttcaccttc ggtttagaaa ggataatttt gaccaggtcg atgtatgtaa caggtttata
    11641 gaaataataa ccctgctgaa atgtgatatt attttggttc agatagtcaa gttgttcttt
    11701 cgtttcgaca ccttcagcga cgatactgat cgaaagttta cgcgccagat ccaatacaca
    11761 atccagaatt cgggttgatt ctgggtctgc gtttacgcgg ccaacgaaac tgtgatcaat
    11821 tttgatataa tcaatatgca ggtcatgaag ataagaaagc cctgagtagc cagtaccgaa
    11881 atcatccagc gcgatgacaa aaccattttc atgcagaatg ttcaaccgct gaaccagact
    11941 ttcatcaaca ttcaatggct cacgctcggt gacttccaga acaaggttta aatcgcggcg
    12001 ggtaaaactg tcacggaaat ttaaacactc gtcgacaaac gtcggcgaaa taatatgcga
    12061 agcgctaaaa ttaattccaa tatgaaaacc ttccggcaat ttactcgcga tagcgttcat
    12121 ctgtctggca acctgattca tcaggctttg cgtaagcgga acgattaatc cggatttttc
    12181 agcaagtgga ataaatgccg cgggtgatat atatccaccg tgaggttgtt tccagcgggc
    12241 taacacctca actccccgca atgtcccttc ccgaccattt accacaggtt ggtaaaaagg
    12301 gacgatctcc ccattattta ttgcccgtcg caggatctct tcaggcgttg cgcttttatt
    12361 gaaatatcta tcaagcagga aggcagcggc gcaggcgatt aacagaataa atattaaaat
    12421 tccaaagcct ttatcgataa gtctgtagaa gctaaagagt ggcggcgggt taaagataat
    12481 agtgaaatga tattttgtgg agtgaacctt ctcggtatag ggataatgtc cattaagcgt
    12541 catcacatca cccgttggtc caataatgtc atccgccacg cgtaatacat accttacccc
    12601 tttcaagggt acattaagcg ccccgcgaat atgttgatca cttatcgtaa ccaaaatgcg
    12661 cgtatctgca aattggttct gatagagcag gataggtaat ctattaacgg tgtcgattgc
    12721 tggagccaac agtaaattac tgtccgggaa aacaggaata cgagacaaca ggacccgatt
    12781 cccaggcagg gatgtacacc agactattcc ctgtttgaga atgatgattg tgcgcaggtg
    12841 aggtttcaga gctgcttcag tgccaagctg atattgctcc tcgaggttgc attccttccc
    12901 ggcaatgtta accgctgttt gagtcgcttg tgacgcttct tcaagtatat gattgatatt
    12961 gttggcggca tatctcgctc ccgccaggta ctctgctttt gcggaatacc agagctggat
    13021 gttcaaaata gcgacaccgg taataaacaa gcatatcgca acgaggatgg gtatgagtgt
    13081 attgcgcatt tgcttgtccc ggccaaaggg tttaatacat gaaaatgcca ttcattcgct
    13141 cgcgctggca tctacagaga gcatggtaga gagcataaga aatatccacg ccgaagataa
    13201 aattttctcc atcttgatct aacccattaa ttattgagca taatgtaggc agtacaaaat
    13261 aagttaggcg ggatatcagg cgtcaagaat ggagagcgag aactctccat tcttgacacc
    13321 tgatattgcg gacataataa gaaagcataa cgcctgaaat gctcactttg catcagcatg
    13381 gtgatacagc tgatgtttat tctaaaacct tactcaagtt ctaagagagc acggattccc
    13441 tgtcattaat aatgatatcc gttagtgtag tcttatgcat ttcaatgtac tgcaatattt
    13501 gcagatagag atcagaaaga atcacactgg tttcatcttt taaacgcaag cgtaaagcct
    13561 gatcaataat atgactatca acatacccat gcgtcatgaa cacctccctc tcgagatttt
    13621 taaacaaagc acattgaaaa ataaatctct ctgggattga attattcatg tcagcctcgt
    13681 agaaatatta acccaataaa attatgctgt gtatagtcta aagcgacaga caaaaataag
    13741 atgaatgagt tttctataaa cttatactta ataattagaa gttacatatc atcagctgtg
    13801 tatcgcaaca cgatttccag tgtttttcta atcacatcag ctttgagaat atcacttgtt
    13861 gtttccaggg agtgtatcag acttaagata atatttttat tattaacatt atcgcctgaa
    13921 agcattaaat tggtgacagc ctgccctaac actgctgact cttcttctaa caaattaccc
    13981 gaactgcgaa agtaacttgc taacgcttta tcagttattg cattatgaat tgtagtatct
    14041 t
//
LOCUS       DAZFXT010000119        13883 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.119, whole genome shotgun sequence.
ACCESSION   DAZFXT010000119 DAZFXT010000000
VERSION     DAZFXT010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13883
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.119"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            258..1088
                     /locus_tag="ACHKW0_003822"
     CDS             258..1088
                     /locus_tag="ACHKW0_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HFO7476577.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPKVDSELTRKYERARLDKENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKEPWPFNYVTETCRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLSLFMKSPSYPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIMTVSGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1229..2002)
                     /gene="uxuR"
                     /locus_tag="ACHKW0_003823"
     CDS             complement(1229..2002)
                     /gene="uxuR"
                     /locus_tag="ACHKW0_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HFO7476578.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDNSGSQNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2217..3677)
                     /locus_tag="ACHKW0_003824"
     CDS             complement(2217..3677)
                     /locus_tag="ACHKW0_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HFO7476579.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFSLMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKVNAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3758..4942)
                     /gene="uxuA"
                     /locus_tag="ACHKW0_003825"
     CDS             complement(3758..4942)
                     /gene="uxuA"
                     /locus_tag="ACHKW0_003825"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HFO7476580.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5282..6625
                     /gene="gntP"
                     /locus_tag="ACHKW0_003826"
     CDS             5282..6625
                     /gene="gntP"
                     /locus_tag="ACHKW0_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HFO7476581.1"
                     /translation="MHVLNILWVIFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTITNIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6868..7770)
                     /gene="fimH"
                     /locus_tag="ACHKW0_003827"
     CDS             complement(6868..7770)
                     /gene="fimH"
                     /locus_tag="ACHKW0_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO7476582.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7790..8293)
                     /gene="fimG"
                     /locus_tag="ACHKW0_003828"
     CDS             complement(7790..8293)
                     /gene="fimG"
                     /locus_tag="ACHKW0_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HFO7476583.1"
                     /translation="MKWRKRGYLLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8306..8836)
                     /gene="fimF"
                     /locus_tag="ACHKW0_003829"
     CDS             complement(8306..8836)
                     /gene="fimF"
                     /locus_tag="ACHKW0_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HFO7476584.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8846..11482)
                     /gene="fimD"
                     /locus_tag="ACHKW0_003830"
     CDS             complement(8846..11482)
                     /gene="fimD"
                     /locus_tag="ACHKW0_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HFO7476585.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLFVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTTMIQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11549..12274)
                     /gene="fimC"
                     /locus_tag="ACHKW0_003831"
     CDS             complement(11549..12274)
                     /gene="fimC"
                     /locus_tag="ACHKW0_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418736.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO7476586.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12311..12850)
                     /locus_tag="ACHKW0_003832"
     CDS             complement(12311..12850)
                     /locus_tag="ACHKW0_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476587.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLAYQ"
     gene            complement(12915..13463)
                     /gene="fimA"
                     /locus_tag="ACHKW0_003833"
     CDS             complement(12915..13463)
                     /gene="fimA"
                     /locus_tag="ACHKW0_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7476588.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
BASE COUNT         3413 a         3530 c         3332 g         3608 t
ORIGIN      
        1 ggcattatta atatagacat caatatagca acatcaaatt ccaattagta ataagatatg
       61 ctttttaatt gcatgcaaat aattttaaat gagcgttttt tgatacttta gagtgtttcc
      121 taccctttaa tatcaccatg tagggttgat atcactacaa cagaaacatg catgtcattt
      181 gtttacttct tttttactac agaaaaattt atctattttt ctaaacatca gtgaaatact
      241 cataaaaagt aactattatg tctatgccat taagcaatgc ccttcagagt caaattatta
      301 cagataatca cttccttcat catccaaaag tcgacagtga gttaacacgt aaatatgaac
      361 gtgcacgcct ggataaggaa aatatctatc ttctgcctct tgctcgtggc aataaccata
      421 attacgatgg aaaaagcgtt gtcgaaatta gaaaacttga tattagtaaa gagccctggc
      481 catttaatta tgtgacagaa acatgcagag aatccgatgg tattacaaca acaggacgta
      541 tgctctatcg aaacttgaaa atcacctcag ctttagacga aatatatggt ggtatatgta
      601 aaaaggcgca tgctgccaca gagttagcag aaggattacg gctaagttta tttatgaaga
      661 gcccttccta tcctgttgag gactatacag ttcatgaaat aacattaggc cctggttgca
      721 atgttccagg ttacgccgga accacgatag gatatatttc aacacttcca gtttctcagg
      781 caaaaagatg gaccaatgag caaccaagga ttgatatcta tattgatcag ataatgactg
      841 tctcaggggt ggcaaactct tccggattcg cccttgccgc attacttaat gctaatattg
      901 agttgggtaa tgacccaata attggtatag aagcctatcc cggtaccgcg gagatccatg
      961 caaaaatggg atataaagtt atcccgggtg atgaaaatgc accactcaaa cgtatgactc
     1021 tgcaaccgtc atctcttcca gaactgtttg aattaaaaaa tggtgaatgg aattatattg
     1081 gtaaatagcc actgagaaga atgagtacta acattcaggt tatacagata gctaaacaat
     1141 accctgcaat ttacaaagta atactcattg cgacttctta atttaagaaa gcgcaaggaa
     1201 cgtttaccct tgcgcttatt ataataagtc aggcgtcgac tttatccagc ggccatgaat
     1261 caaacagata gccatcaaaa taaatatcgt caacgttcga gaattccagc agacgttgct
     1321 taacgttttc cagatgctgc cacattgcca gcttcgccgc tcgggcatct tttttgatta
     1381 acgcggcgag gatctgtttg tgatcgccca accactcttt gcgatacagg ctgtcatcca
     1441 gatggctgtg caactggatc cacattggat tgttttcccg ccactgccag gactgacgga
     1501 acagctccac cagcatgctg ttatgcgttg cttcggcaat agcgagatgg aactgcatgt
     1561 caccgctttc gctgctgccc ggcgcactgg aagccagttc acgctcttcc agttgcaatg
     1621 cctgacgcat tttgacgata tcttcgcggg tagcctgcaa agcggcaaac tcggcgatgt
     1681 tgctctccaa taactgccgc gcctgtaaca gctcaaaagg accggcatcg ttgcagacgt
     1741 tggcatccgg actgtctgtg ttctggctgc ctgagttatc aagaacatag ataccggcac
     1801 cccggcgtac ttccaccagc cctttgatct ccagcatgat cagcgcttca cgtaccaccg
     1861 tccgcgtgac atcaagcatt tctgcaattt cacgctccgg cggcagccgt tcgccaggat
     1921 tgtacggcgt ctttatgatc agatcgcgga tcatcgcccc gacttcctgg taaggtcttt
     1981 gcgcagaggt ggcagatttc atggtgttgt gtccgttagg tgcggttaat caaatttttg
     2041 ccggatgcgg cgtgaacgcc ttatccggcc tacaacgggc aattttacgg cccccgttaa
     2101 tctcaaacta taccattagc actggaagtt ggcacgtata ggtgggcaaa aaaacgaaaa
     2161 ctggtcaacc aaatatcagt ttgattgacc agtgagtctg tagggtaatc agttagttac
     2221 agcgcagcca cacactcgcg cgcaccgcgt tcgcacagct gctgatatgc cgccgtcact
     2281 gcgccaacaa agtcggcatt ctgcggcaga tcatcggcaa aaataccgct caggcccagc
     2341 agcgctttca cgcggtctgc gccctgatac tgcgcgttga ccttctggaa ctccgccagc
     2401 atcgggtcga ccacgtcaat ggcattaccc tgctcatcca cgccctgggt gtaacgcatc
     2461 cagccagcca cgcccagcgc caggtgacgc cagctgccgc cgttttgcag gtgcagacgc
     2521 accgggtcca gcagacgctg cggtaacttc tggctgccgt ccatcgcaat ctgccaggta
     2581 cggtgacgca gagacgggtt gctgaaacgc tcgatcagca gcgtcgcata ggcgttcagg
     2641 tccgtacctt ccggcatcga cagcgttggc gcttgttcct gcatcatcaa ggagaaggcc
     2701 gctttgcgat aatccgggtt agtcatggtg tcggcaatgg tttcatagcc gccgaggtaa
     2761 ccgaggtatg ccaggaaaga gtggctgccg ttcagcatac gcagcttcat catttcgaac
     2821 ggcacaacgt ctgcaacgaa ctgtgcgccc actttatccc agtccgggcg accgttaacg
     2881 aagttatctt caatcaccca ctgacggaac ggttcgcagg caatggcgca cgggtcgtaa
     2941 acacccagct ggtcagcaat ttcctgcaag gtttctggcg tcgccgccgg aacgatgcgg
     3001 tcaaccatgg tgcacgggaa ggtgacgttt tcttcaatcc atgccgccag ctgcgggtca
     3061 cgcgcctgag ccagtcccag taccgcgacc ttcgccacat ggccgttttc acgcacgtta
     3121 tcgcaggaca tcaccgtaaa cgctttcaac cctttttcac gacgcagccg cagggcttcg
     3181 acgatgtaac caatcgcgga cttcggcgca gtcgggtttt ccagatcgtg cttgatcagt
     3241 gggttattga gatccagctg accgcttgcc gcatcagcgc agtagccttt ttccgtgact
     3301 gtcagagaga caatcgccgt ctgcggacgc gccatcgcat tgagaatacc ttcgcagccg
     3361 tcgatttccg gatgcagcgc ttctttcatc gaaccgataa ttttcagctc ggtgctctct
     3421 gcgccttttt ccgctacggt gtacagcagt tgctgttttt tcaggttttc gatcagcacg
     3481 cggtcgttgc ctggcatcag gttaacttcg cagatgcccc agtcgctgtc ggtgctttcc
     3541 agcagatgat gggtatacag cgcctggtgc gcgcggtgaa acgccccgca accgagatgc
     3601 acaatgcgtg attccagacg agaatgatcc catgacgggc gggcaaccgg cagattgctg
     3661 tcaacaatag tagtcatcgt aaactccaga atccgtcttc aaagagtgac ggcaaggaag
     3721 aaggaaccgg cacggcgcgc agccatgccg gtggatatta acggctaaag aaagcgcgct
     3781 ggatcgccag ttcgacaccg cgaacttcgg ccaggccttt cagacgacca attgcggagt
     3841 aacctgggtt ggttttcttc ttcaggtcgt ccagcatctg atgaccgtgg tccggacgca
     3901 tcgggatcag gtcttctttg ccttccgctt tacgacggtg ttcttcttca acaatcgctt
     3961 tcaccacttc gtacatatca acgtcaccgt tcaggtgcgc cgcttcgtgg aaggttttcg
     4021 ggttatcttc acgcatggtg gagcgcagat gggtgaagta aatacgcgga ccgaactgct
     4081 tgatcatatc aaccagatcg ttgtcagcac gcacgccgta ggaaccggtg cacatggtga
     4141 aaccgttcgc catgctgttt acggtatcaa ccatccactg catatcttca atggtggaaa
     4201 caatgcgcgg caggccgagg atcgggcgcg gcggatcgtc cgggtgaaca gccatacgga
     4261 cgccaacttc ttcggcaacc ggaataatcg ctttcaggaa gacagcaaag ttttcgcgca
     4321 gtttggcttt gtcgatatct ttgtacagtt ccaggtgctt acggaactgg tcgagggtgt
     4381 acccttcttc cgcacctggc agaccagcga tgatgttacg ggtcagacgc gctttgtctt
     4441 catcgctcat ggtggcgaag cgaacagcag cctgagcaat ttcttcttcg gtgtaatccg
     4501 cttccgcgcc tggacgtttc aggatatgca tttcgaatgc agcgaattcg atctggtcga
     4561 agcgcagagc tttggagccg tctggcagca cgtattcgag gtcagtacgg gtccagtcga
     4621 gcaccggcat gaagttgtag cacacggtgc gaatgccgca ctgcgccagg ttgcgcagag
     4681 tctgctgata gttagcgatc cactgctcat agttgccagt gtgggttttg atatcttcgt
     4741 gaattggtac gctttctacg acagaccaca ccaggcctgc gtcttcaacg atcgccttgc
     4801 gtttgaggat ctcttctacg gaccatactt cgccgttcgg gatatggtgc agcgcggtaa
     4861 ccacgccagt tgcgcccgcc tgacggacat cagctaaaga aaccggatcg tttgggccgt
     4921 accagcgcca ggtctgttcc atgatttttc ctctttcaca tagtgggttc cacagcaaag
     4981 agacgcggaa ccctggagtt gtttaggtat ccggatgaac aaccagaccg cagccgtggc
     5041 gttgcatcgg atgagatggc gtataagttc taccttttgg taagacatgt caacccgaat
     5101 tctgaaattg gttaaccaca tcacaagaat ttcactctca tggttgttgc tgcaaattgc
     5161 agcaaagggt aatcgatggt tattgatcag aggatgtgac attcatcgca acaatggttg
     5221 accaatttac ataacatgtc gcccaaaata acaacggttc aaccaggtaa ggtaaaacgt
     5281 tatgcatgtg cttaacattc tttgggtaat attcggcatt ggcctgatgc tggtactgaa
     5341 tttgaagttc aaaatcaatt caatggtggc tttgttggtg gcggcgctgt ccgtcgggat
     5401 gctggcgggc atggatttga tgtcgctgct gcacaccatg aaagcgggct tcggcaacac
     5461 gctgggggaa ctggctatca tcgtggtgtt cggtgcggtc atcggtaaat tgatggtcga
     5521 ctccggcgcg gctcaccaga tagcacatac gctgctggcg cgtctcggtc tgcgctatgt
     5581 acagctgtcg gtgattatca tcggcctgat tttcggtctg gcgatgtttt atgaagtggc
     5641 ctttatcatg ttagcgccgc tggttattgt tattgccgcc gaagctaaaa ttccgttcct
     5701 gaaactggcg atcccggcag tagcagctgc cactaccgca cattcattgt tcccaccgca
     5761 gccgggtccg gtggcgctag tgaacgctta tggcgcggat atggggatgg tttatatcta
     5821 tggcgtactg gtgacgatcc caagtgtaat ctgcgcaggt ctgatcctgc cgaagttcct
     5881 cggcaatctt gagcgcccaa cgccatcatt cctgaaagca gatcaaccgg tagatatgaa
     5941 taatctgccc tctttcggcg tttcgattct ggtgccgctg atcccagcga tcattatgat
     6001 ctccaccacc atcaccaata tctggctggt aaaagatacc cctgcgtggg aagtggttaa
     6061 ttttattggt tcctcgccga ttgcgatgtt tattgcgatg gtggttgcat tcgtactctt
     6121 tggcaccgcg cgtggtcatg acatgcagtg ggtgatgaac gcttttgaaa gcgcggtgaa
     6181 gagtattgca atggtgattc tgatcatcgg tgcgggtggc gtgttgaagc agaccatcat
     6241 cgacaccggc attggcgaca ccatcggcat gttgatgtcc cacggcaata tctcgcccta
     6301 catcatggca tggctgatca ccgtactgat tcgtctggcg acgggtcagg gtgtggtttc
     6361 agcgatgacc gccgccggga ttatcagtgc tgcaatcctt gatccagcaa ctggtcagct
     6421 ggttggcgtg aatccggcgc tgctggtact ggcgacggct gcgggttcca acaccctcac
     6481 ccacattaat gatgcctcat tctggctgtt caaaggttac tttgacctgt cagtaaaaga
     6541 cacgttgaaa acctggggtc tgctggagct ggtcaactcc gtggttgggc tgattattgt
     6601 gttgattatt agcatggtag cgtaaaagga caccagagcc agccaatggc aggctcagac
     6661 tgatgacaaa gtctaaaaat gtgcccggac agtcccctcg cccctccggg gagagggtta
     6721 gggtgagggg aacagaccag caaagtgcgc tttgtttatg ccagatgcga cgctgacgcg
     6781 tcttatctgg cctacaaagg gctaacgtgc aggtttttag cttcaggtaa tattgcgtac
     6841 cagcattagc aatgtcctgt gatttcttta ttgataaaca aaagtcacgc caataatcga
     6901 ttgcacattc cctgcagtca cctgccctcc ggtacgtgca taatttgccg ttaatcccag
     6961 actcaccgcc gaagtcccta ctgctcctaa agataccgtg ttattcgctg gaataatcgt
     7021 accgttgcgc gtcaactgta cgccgacgcc ctgtgcaggt gaaaacgacg cggtattggt
     7081 gaaaatcgag ttgcccgcat ctgcggttgt gccggagagg taatacccca ggttttggct
     7141 tttcgcacaa taaacggtaa gaggaattgg cactgaacca gggtagtccg gcagagtaac
     7201 ggtgacatca cgagcagaaa catcgcagcc gccagtaggc accaccacat cattattggc
     7261 gtaaatattc cacacaaact ggaaatcatc gctgttatag ttgttggtct gtcgcaaaat
     7321 aagcacggca attaatgagc cagctttaat cgccaccccg cccgcactgc tcacaggcgt
     7381 caaataaagc gccaccggcc acggcttatc cgttctcgaa ttataaacaa cgcgcggcgt
     7441 ttcgctggtg gtaggaaatg gatagctact gccactatat tttacggtcc cggaaaaatt
     7501 agataacacg ccgccataag ccgagcctcg ttgcagtgtg acatagtctg taatggtttc
     7561 cggataatcg ttatggcaaa agatttgcgt cgaaagatcc acgaccaggt tttgccccac
     7621 attcacggcg ggcgcaaggt ttacataaac attggcgctg ccaccgccaa tagggatagc
     7681 ggtaccattg gcggttttac aggcgaatga ccaggcattt accgaccagc ccatcagcag
     7741 tacagcaaac agggtaataa ctcgtttcat tacaatcatc tcttcgggtt cagctgtagg
     7801 tataggtgat gctaatcact gcctgaatgg ttccctgagt ggctccgcca tttaccgtca
     7861 atgctctgac ctgtaacggg aagtgcgctg attgtgagga atcatccacc tgaactgttt
     7921 tggttgcgcc agtattcaat gtgttgccac tgtcatcctg tagctctaac tggatgtttt
     7981 gcgcggtccc ctggttttta taatatccgg tgctgtcggc tgccccgctg aagctggcag
     8041 tgaccctcga cgttcccacc ggacaattag tcaactcaag cgcaacatca tgccaggccg
     8101 atgccgcccc ggcagacata agactgaaag aataaagatc gccgagatca accgtggcat
     8161 tggtggtgga aactgtgcac ggtttggcga cgaccttacc gtttaccgtg atggtgacat
     8221 cggctgcctg tatcgtcgca cttgcgagcg ccaatattgc cgccaataaa tacccacgtt
     8281 tgcgccattt catgagcatc tccagttact gatattcaag agtgaaggta gccgtggcat
     8341 tgatatgccc cgcagtgaca ggcacctgtg tcgccattag ccgggcgtaa aaattcaacg
     8401 tatttggttt acccggcgtc agggtcgtcc acgaaatcgc ggacgatgga gcattaaggg
     8461 gtatttgatt ttgctgctca ttcagaagct gtattcccag tcccgaagcc gctgacaccg
     8521 tattttcaag tgcaagcaga ttggcattgt ggctatctgc aacgccggta aacccaactt
     8581 ttacggcaga aacggcatta ccacagggtg acagcaaaat acgaaatgga acaacaggag
     8641 tcgtcgcgcc aatgttgtta aattgcttcg ccgcgttttc catcagatca acagtaaaat
     8701 tggttgattc agcggccaca ctacagccgt tatctctgac atagccgcgg atagtaatcg
     8761 tgctatccgc agccaaagcg tgactcaccg ccagccacaa aaaagcgcac agaagataaa
     8821 aaggtttgtt tctcatcacg cccccttaac gacattcagc tgatagctgg gttaataact
     8881 gctgctgact ctctggtggc agttgataat tggcgacaca gtgagcattt tcctcttctc
     8941 cccatttcac ctgaactttt cccgctaaag gcattccgct gaggtaaacc tgaccattat
     9001 ccgcaacaat gccgctactc tggctactct ctgatgtcac catcgcccca aacggcagcg
     9061 gcttattatt gtgggttagc gtcatgagca gttttatccc aacgcgcgct ttaaactctg
     9121 ctcgcacgat cgccccacga gtgggaacaa cgttagcgac cgcgttatct aaatcgacgt
     9181 tatcagccag ggtattggta tccagcgcca ctctgttttc cctatattca gtggcataag
     9241 gcagcacggc ataaccgcgc cagtcggtac gcacccccgt ctggttttcg acttttgcat
     9301 cttttgcgcc aggcgcttta acaagcacca ccgtatcgtt taacggctgc cccagcgtta
     9361 cgccattggc atgagccaat accccaccgc tgactccgta atagagctgc ttaatatcat
     9421 cgctatggct gtaaccgata ttggcattgc cgtaaccacc gcgataattc agcgtggcgt
     9481 agcctgtgct tccgctatta ccatcgcctc ccccggcata gccggtttgc acgctatagc
     9541 tgaggttgtt gtcttccagc aacgtaccgt atacaccagc cagattggtc atccgaccgt
     9601 tgagatcgtg tgacatgctg tagctggcac tggcatgtcg ccactgagat ttactgtcag
     9661 aacgcagcca gtggctgaaa ggaatattga cgttaagcgc taacatctga tcacgtcctt
     9721 tttgccaggc gtttttcgtc aggctatagc tgagcgtcca gttgatatct tcgaatgcag
     9781 tatttaatcc agcctggaat tgctcatcga cattactcgt tccccaataa gtttgatggc
     9841 taccactcaa atacagtgtt gatgtgcgcc cgagttgctg agtaacggtg agctgtaatt
     9901 tcccgcgttt gttataagcg aggttgtaat agtcggtgaa tttcggctta acctgaataa
     9961 ctccgtcctg tgtttcgatg ttgtagccat tcattcgact gtatgttgta tcagcgaaat
    10021 taaaatatcc gctggtcgaa taacggtaac ccactaactg aatattcgtg cctgactcat
    10081 tgagcgattt gttatagaga aaacgcaccg attgtccgtc atgctgactg tcatcgggaa
    10141 gtgtggaatt agcctgcgtc atatccacag acagagcgcc cagtgccccc atgtttttcc
    10201 cgataccgaa attaaaagca cgataacgat ccgccagttg cgttccacca tatattgtcc
    10261 agccagccgg aaggccgtgg agtaatgtac tctggaaaaa gcggggtttt tcctgctgcg
    10321 catttccact acggtattct cctgccgtaa tggaataacg agtatgccct tcacgttgca
    10381 aaagcgggac tgacgaatag ggtacggtaa aaatctgcgt gctgccgtca gcctctttga
    10441 tcgttacctg caagtcacca ctattacctg cggcatagat atcgttgatg gtaaaaggcc
    10501 ccggtggcac cgtactatta taaatgtcat acccattttg tttaatagtg acctgtgcag
    10561 taccacgagc aataccgtgg atcaccgggg caaatcctct ttggctatcg ggtaacatat
    10621 tgtcatctga ggccaattgt gcgccgcgaa agttaatacc atcgaaaata tcgccctgag
    10681 tataaccatc acccagcgtc agccgggaac gtaacggtat tatgtctcgc tcaagccagg
    10741 tattgatatg ctgccattta tttttgctac ctgatgatct gtcgctactg ttataactcc
    10801 aggtggtatt gtcgcgtaaa cgccacgcac caatatttaa cccactctgt aggtttaaat
    10861 atgcataatg gctgttaccc ccaatccgat tctgtacact atttccgctg aaattataat
    10921 tgagcaatcc ggcattaata ccgggatccc ataactcagg aggaatataa ccacgcgcgc
    10981 gattactcat aaatgcctga gggatcgtca ggttcagtcg ctgctgacca acatccagat
    11041 gcgcagtagc gtcctggatc attgtggtta atggcacgca ggcatcatcc gccagcagat
    11101 tcataccgga gacagaagcc gtattcagcc ccatactggc aagttgcgcg cgtgtcaggc
    11161 agggaacaat cccttgttca ctgtcgcccg tattaaatgt gacatcacgc gttgccatat
    11221 aaccattatt caaatagata tcgacacgat acgtccctgg cggtaattct tgcccatttt
    11281 caaaacgcga taaatcggcc acagcctggg gatcatccgc taaaaaacgc gggttaaaat
    11341 agagttcggc agatgacaaa ggtgcctgtg cggcaaaagc acaggcaaca aagagccgga
    11401 caaaaaaacc agccaaacga tgcttacgaa tatgcaagca ttgtgtgttt cgctggtaaa
    11461 gtcttaaatt cagatatgac attattcctc cggtttaaag tcacccggca gtcaattctt
    11521 tttattcagg cgaaaaattc cccctgcgtt attccattac gcccgtcatt ttgggggtaa
    11581 gtgcaccata atcatttatt gttcggtaag taatattgct tcccgcatca gaaggcaatt
    11641 taaccgtgct ttcgcccatt ggaggcacca aggcattttc aagaacccgg gttccggcat
    11701 tcaactctgt taccgtcagg taatagggtg tcgggttaat cagcgtcaga gaattcgcgc
    11761 tacgacgaaa tcttaatttt tctgcggcct gatcgggtgg caacgctaat ttagccgggc
    11821 gatagtacag tttaatgcgg ctgataattg cgagctgtag cgtattctca gtcaattttg
    11881 atttatccat tgacggaatc gctttaacat tcatccagaa taaactttcc cgatcctgtg
    11941 gcaattggtt atttgttgca tcaagaatac gcaaggtatt ctcttttttt cccttcatcg
    12001 caaacagagg aggcgtcacg ataaaacgac catcctttac accatcggca ttttccaccc
    12061 atgattgaat taaataggta ctattttcat cattatttgt cacggcaagt tgcacttgtt
    12121 tttgcccggc cggataaatt acgcgagtcg cacctaaggc cactcccgct tcagcgcgtc
    12181 cggcaaccat cattgccata aacatcagga tacctgccag caagcagaat gttatttcct
    12241 gcgatttcct tacattgacg tttttattac tcactgtcct gttcctgtta tcacattatc
    12301 tgctgaacaa ttactgatag gctaaagaga accaggcctg ggcattggcg atgccgccag
    12361 taacccgacg cccggtagca cgatatttgg cgatgaaatg tagcgaagta gagcctgagt
    12421 aaagccgttt ccagtttgct ggaggacgat taatcggtac gaggtttcct tcatcatcaa
    12481 acaacgctac gccaatattg gtggctatcc ctggcccctc tcccacggaa agcacatccg
    12541 gatttttacc atccgcgaca ccgtgaaacg ccacccccac acgttcactc accaccgtgc
    12601 tacattcccg taaatgaata acaaaaggca ccggtgcgct atcttcccct gccgcatgaa
    12661 accggttact gctgatttgc cccatattga ccgtcatttg tttatcaccg gcttcaatcc
    12721 ggcaagtttc cgcaataatg acgccctgaa attgcatatt tccgccaggc agcgtggtat
    12781 tccatttatt tccggccaga gcaaacattg gcagcaacga cgctaacaga aataatcttt
    12841 tccttttcat cgttattttt atcgcacaag ggtgggcatc cctgcccgta atgacgtcct
    12901 tgaacctggg taggttattg atactgaacc ttgaaggttg catccgcatt agctgcaccc
    12961 ggggttgcct cgccgattgc ataataacgc gcctggaacg gaatggtgtt ggtaccgtta
    13021 ttcagggttg tttgtgcact aaatgtcgca ccgtccagcg tcagagcatt gcctgttctg
    13081 tccaggatct gcacgccaac gtttgttgca ctacctgcag cagaactctg cagagccagt
    13141 acatcagtac gcgtagcatc aattgccgta cctaagaagg caacagcggc ttttgtggca
    13201 acagtggtat cgcaatcatt cagctgaatg ttaaaaccaa cggcagagct ggttgctcca
    13261 gcctgcttca ggctagcggt acgaacctgt cccaactgaa cggtttgatc aacagagcct
    13321 gcatcaactg cgcaagcggc gttaacaact tcccctttaa agtgaacggt cccaccattt
    13381 accgtcgtag tatcggccag agccgctgcg gaactgaggg acagagccga cagaacaacg
    13441 attgccagag ttttaatttt catgctgctt tcctttaaaa aaactatttc taaatcgaca
    13501 tgggcagtcg ttctgtacac tttgttttgt caacgagttt gactgccaac actgcacagt
    13561 tttcccccaa aagatgaaac atttggggcc attttgactc atagaggaaa gcatcgcgca
    13621 caaacttttt cagtttattt gttggcttaa tattctattg ttatctttat ttatagattt
    13681 ttatattgca tgatgtggtt tttggagaga agaatgagga agatgcgtcg agccacagaa
    13741 acgttagctt tacatatagc ggaggtgatg tgaaattaat ttacaagaga aataatttac
    13801 atatcaaaca gttagatgct ttttgtcgtt ttttaatatt tttatgcttg agaaaaaata
    13861 cgtaacttat ttatgatatg gac
//
LOCUS       DAZFXT010000120        13683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.120, whole genome shotgun sequence.
ACCESSION   DAZFXT010000120 DAZFXT010000000
VERSION     DAZFXT010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.120"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..562
                     /locus_tag="ACHKW0_003834"
     CDS             <1..562
                     /locus_tag="ACHKW0_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HFO7476589.1"
                     /translation="AVISEELATAAASRQQQALLTRLAELAADSSADDAAAINALRQQ
                     IQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQPF
                     TPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVGL
                     ADSVWSETPDALNADILQTLTQRIPQ"
     gene            562..2658
                     /locus_tag="ACHKW0_003835"
     CDS             562..2658
                     /locus_tag="ACHKW0_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HFO7476590.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRVTENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(2705..2983)
                     /locus_tag="ACHKW0_003836"
     CDS             complement(2705..2983)
                     /locus_tag="ACHKW0_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476591.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            2999..3127
                     /locus_tag="ACHKW0_003837"
     CDS             2999..3127
                     /locus_tag="ACHKW0_003837"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476592.1"
                     /translation="MVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYPAYG"
     gene            3253..3663
                     /gene="psiE"
                     /locus_tag="ACHKW0_003838"
     CDS             3253..3663
                     /gene="psiE"
                     /locus_tag="ACHKW0_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HFO7476593.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(3707..5182)
                     /gene="xylE"
                     /locus_tag="ACHKW0_003839"
     CDS             complement(3707..5182)
                     /gene="xylE"
                     /locus_tag="ACHKW0_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HFO7476594.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLNESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(5554..6444)
                     /gene="malG"
                     /locus_tag="ACHKW0_003840"
     CDS             complement(5554..6444)
                     /gene="malG"
                     /locus_tag="ACHKW0_003840"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HFO7476595.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(6459..8003)
                     /gene="malF"
                     /locus_tag="ACHKW0_003841"
     CDS             complement(6459..8003)
                     /gene="malF"
                     /locus_tag="ACHKW0_003841"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HFO7476596.1"
                     /translation="MDVIKKKHWWQSDALKWSVPGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(8157..9347)
                     /gene="malE"
                     /locus_tag="ACHKW0_003842"
     CDS             complement(8157..9347)
                     /gene="malE"
                     /locus_tag="ACHKW0_003842"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015144 - carbohydrate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HFO7476597.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            9712..10827
                     /gene="malK"
                     /locus_tag="ACHKW0_003843"
     CDS             9712..10827
                     /gene="malK"
                     /locus_tag="ACHKW0_003843"
                     /EC_number="7.5.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HFO7476598.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQLEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            10899..12239
                     /gene="lamB"
                     /locus_tag="ACHKW0_003844"
     CDS             10899..12239
                     /gene="lamB"
                     /locus_tag="ACHKW0_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HFO7476599.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAVTRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAYVDEKFSYDINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTLMEIGYDNVKSQRTGDTNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYTDKAKGTPNYGKAVPANFEGGSFGRGDSDEWTFGAQMEIWW"
     gene            12482..13402
                     /gene="malM"
                     /locus_tag="ACHKW0_003845"
     CDS             12482..13402
                     /gene="malM"
                     /locus_tag="ACHKW0_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HFO7476600.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAASAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3390 a         3520 c         3582 g         3191 t
ORIGIN      
        1 tgcggtgatt agcgaagagc tggcaacggc ggcagcatcg cggcagcagc aggcgttgct
       61 gacacggctg gcagaactgg ctgcagattc cagcgccgac gatgccgccg cgattaacgc
      121 cttacgccag caaattcagg cgttgaaagt gacgggcagg caaaaaatca atcttgatcc
      181 tgatatcgtc cgcgttgccg aacgcggtaa cccaccgttg cagggcaact acacgctgtg
      241 ggtcgggcca ccaccgtcca cggtcacgct gttcgggctt atcagccgtc ctggcaagca
      301 gccattcact ccaggtcgcg acgtggcgag ctatctctcc gggcagaacc tgcttagcgg
      361 tgcggatcgc agctacgcgt gggtggttta cccggacgga cgcacgcaaa aagcgccggt
      421 ggcttactgg aacaagcgtc acgtagagcc gatgcccggc agcattattt atgttggcct
      481 cgcggactcc gtctggagtg agacgcctga tgcccttaac gccgacattc ttcagactct
      541 gacgcagcgg ataccccaat aatgaaaaaa agacatctgc ttagcttact ggcgctgggc
      601 attagcacag cttgctacgg cgaaacatat cctgcgccca ttggtccgtc gcagtcggat
      661 ttcggtggcg taggattatt acaaacgccc accgcgcgca tggcgcggga aggggagttg
      721 agcctgaact atcgagataa cgatcagtac cgttattact cagcttcagt gcaactcttc
      781 ccgtggctgg aaacaacgct gcgctacacc gacgtgcgca cccggcagta cagcagcgtc
      841 gaagcgttct ctggcgatca aacgtataaa gataaagcct tcgatctcaa actgcgtttg
      901 tgggaagaga gttactggct gccgcaagtg gcggttggtg cgcgggatat cggcggtacg
      961 gggctgtttg atgcggaata tcttgttgcc agcaaagcct gggggccgtt cgattttacg
     1021 ctcggcctgg gctggggata tctgggcacc agcggtaatg tgaaaaatcc gctctgttca
     1081 gccagtgata aatattgcta tcgcgataac agctacaaac aggcgggatc tatcgacggt
     1141 agccagatgt tccacggtcc tgcctcactg tttggcggcg tggaatacca gacgccctgg
     1201 caaccgctgc gcctgaaact ggagtatgaa ggcaataatt atcagcagga ttttgccggg
     1261 aagctggagc aaaaaagtaa gtttaacgtc ggtgcgattt atcgcgttac cgattgggcc
     1321 gacgttaacc ttagctatga acgtggcaac acctttatgt ttggcgtcac gctgcgcacc
     1381 aactttaacg atctgcgccc gtcctacatc gataacgccc gcccgcaata tcaaccgcag
     1441 ccgcaggatg ccattttgca gcattcggtg gtggcgaatc agttaacgct gttgaaatac
     1501 aatgccggac ttgccgatcc gcagatccag gcgaaaggcg atacgctgta tgttaccggc
     1561 gagcaggtga aatatcgtga ttcgcgcgaa gggatcatcc gtgctaatcg gatcgtgatg
     1621 aatgatctgc cggatgggat caaaacgatc cgcgttacgg aaaatcgcct taacatgccg
     1681 caggtgacga cggaaaccga tgtcgccagc ctgaaaaatc atctcgccgg agagccgttg
     1741 ggccacgaaa cgacgctggc gcaaaaacgc gtcgagccag tggttccgca gtccaccgag
     1801 cagggctggt atatcgacaa atcacgcttt gatttccata tcgatccggt gctgaaccag
     1861 tcggtcggtg gcccggaaaa cttttacatg tatcagctgg gcgtgatggg aacggcagat
     1921 ttgtggctga cggaccatct actgaccacc ggcagcctgt ttgcaaatct tgccaacaac
     1981 tacgacaagt ttaactacac caatccaccg caggactcgc acttaccgcg cgttcgtacc
     2041 catgtgcgcg agtatgtgca gaacgatgtc tatgtgaata acctgcaagc caactacttc
     2101 cagcatctgg gcaacggctt ctacggtcag gtctacggtg gttatctcga aaccatgttt
     2161 ggcggtgcgg gggcagaagt gttgtatcgc ccgctggaca gcaactgggc gtttggtctg
     2221 gatgccaact acgttaaaca gcgcgactgg cgtagtgcaa aagatatgat gaaattcacc
     2281 gactacagcg tgaaaaccgg acatctgacc gcctactgga cgccatcttt cgctcaggat
     2341 gtgttagtta aagccagcgt cgggcagtat ctggcagggg ataaaggcgg cacgctggag
     2401 atcgccaaac gctttgatag cggcgtggtg gtgggtggct atgccacgat cactaatgtt
     2461 tcaaaagagg agtacggcga aggggacttc accaaaggcg tgtatgtctc ggtaccgttg
     2521 gatctcttct cgtctggccc gacacgcagc cgtgcggcga ttggctggac gccgctgacg
     2581 cgtgacggtg gtcagcaact tgggcgtaag ttccagttgt atgacatgac cagcgaccgt
     2641 agcgtcaatt tccgctaagt catgggaaag gtgccagttt tcgcaatcac tggcaccctc
     2701 attcttaatg gcctgttgag ccatcaggaa cgcagttcac ccccggtttg cgatctggtg
     2761 tgtaaaggcg aaaacattct gtcttcctgt ccgtcgatat cggttgcaga tctggttgcc
     2821 tgagcgactg ggccatcatt gggctggaga cattgctttg cggtaggtcg gctttatcag
     2881 cagtaccgag cggaccagca taagcaggaa gaacagagac tgataacatc aaagcagcaa
     2941 aataaggctt catttttacc acctttatca ggttacgttt catttgttcc agaggaacat
     3001 ggtcgatttt tcgcgcattg ctggtggctg ggaatcacct gaatgggtga tttttgaatt
     3061 accggctttg gtgcggtttg tcttgccgga tgcgccgcca ggcgcggctt atccggccta
     3121 cgggtaggta tatccggctt tggtggaggc gcgctccaaa tccaggttga acaaaacata
     3181 cacaaaaaat atagatctcc gtcacatttt tgcgttatac aggaagctcg ccactgtgaa
     3241 ggaggtactg ctatgacgtc actctctcgt ccgcgcgtgg agtttatctc caccattttg
     3301 cagaccgtac tcaatcttgg cctgttgtgc ctcggcctga ttttggttgt cttcctcggc
     3361 aaagaaacgg tgcatctggc agatgtgctg ttcgcgccag aacaaaccag caaatatgag
     3421 ctggtagaag gactggtggt ttactttctc tatttcgaat ttatcgcgct gattgtgaaa
     3481 tactttcagt ccggttttca cttcccgcta cgttactttg tctatattgg gatcaccgcc
     3541 attgtgcgct tgatcatcgt cgatcataaa tcgccactgg atgtgttgat ctactcggct
     3601 gcgatcctgt tactggtgat cactctgtgg ttgtgtaatt cgaaacggct gaagcgggag
     3661 taaaaagaca gcacgccgaa ctggcgcggc gtgctggaca ggaagattac agcgtagcag
     3721 tttgttgtgt tttcttcgtt tccggttccc agagggcttc cagctcctca agggttttac
     3781 ctttggtttc cgggacaaat ttccacataa atagtgctgc cagaacgccc atacacccgt
     3841 aaatccagta ggagaaaccg ttgtggaaat gggccaccag ccaggagttt ttgtccatca
     3901 tcgggaaggt ccaggagacg aagtagttcg ccagccactg ggccgccacc gcgattgcca
     3961 gcgctttacc acgaatggca ttcgggaaga tttccgacag cagaacccag cataccggac
     4021 cccaggacat ggcaaaggcg gcaacataga acagcatcga cagtagcgcc acaatacccg
     4081 gtgcctgagt gtaaaacgcg gtaccgaggc taaacatacc gattgccatt ccgagcgcgc
     4141 cgataatttg cagtggctta cgaccaaatt tatccaccgt cataattgcc agaacggtga
     4201 aggtgaggtt gataactccg acaataatgg tctgcaacag cgcgatatcc gtgctggccc
     4261 ccagcgtttt gaacacttcc ggcgcgtagt acaacaccac attgatgccg acaaattgct
     4321 ggaagatgga gagcataacg ccgattacaa tcacgcccac gccaaacatc agcagacgac
     4381 caccggtttt gcggccatga tccagggagt gtttaatttc ctgtactgcc tgagttgcaa
     4441 gcgtgttgcc cataattttg cgcaggatac tttccgcctg ttcttgcttg ccgcgtgaca
     4501 tcagccagcg aggactttct ggcacggtat acagcagcat taagaacagc agtgcaggga
     4561 tacattccga ggcaaacata taacgccagc cgtcagtatt cagccagctg gcatcaccgg
     4621 aacgggcaat aaaatagttt acgcagtaaa ctaaaagttg cccgaaaata atcgcaaact
     4681 ggttaaatga aaccagtttc ccgcgaatat gagctggagc cagttccgca atatacattg
     4741 gtgaaagcat tgaggctaaa ccaacgccaa taccgccaat aatgcgataa ataacaaatt
     4801 ccgggacata acctgccaga taaacaggca ctgtgttgtc cgggtttata gaggtaaaac
     4861 caagttctgg ccaggcagaa cctacaccag aaataaaaaa caggacagca gcaatcttaa
     4921 gtgaatcacg acgaccgaag cggttactgc aataaccacc gagggcaccg ccgatgatgc
     4981 aaccaatcag agcgctggct acgcaaaacc ctaacaggga gttggcagcg gattcattta
     5041 agttttgtgg agcaacaaag acggtattga gtgactcaac agtaccggaa ataacggcgg
     5101 tgtcgtagcc aaataataaa ccacctaatg tagcgactaa ggtaatcgaa aatatataac
     5161 tggaattata ctgggtattc attcagacct gccttagatc attctgatct tttatcagtg
     5221 cattgatgtt ggaattgtca ggcagttaag gcaattatca ttttttacaa tgcgtttacg
     5281 taatgtcttt tctgtgatct taattgtgat aattatccaa aattgataaa aacaatacta
     5341 ttgccgtgat tcagagcacg aaagagaatt atcgtaagtg ggaaaacaaa taacgtaaaa
     5401 ataataagtt ctatgatggg aatattaacc ggcgaacgat tcagattgca gacgaaagaa
     5461 aaaaaggcgc tccgtggagc gccgaataat agtcacaagt tgggataacg taagttgagg
     5521 gtgcagcggc ataacattgg cagaacaaca tctttaacct ttcacaccac ctgccgtcag
     5581 gccgttgacc agccagcgtt gagccagcaa gaagacgatg gtgatcggta atgcagacat
     5641 cacggcagcg gcggcaaagt caccccacag gtagttttgc gggttgaggt attgctgcat
     5701 ccccacggcc agggtgtagc tatttacgtc acgcagtaac agcgacgcga ccggaacttc
     5761 agtaatggca gcgataaacg acaggatgaa caccaccgcc agaatcggta ctgacagcgg
     5821 caacaggaca aggcggaagg cctgccacgg tgtcgcacca tccagcgccg ccgcttcttc
     5881 cagcgaactg tcgatggttt cgaaatagcc tttgatggtc cagacatgca gcgcaatccc
     5941 acccagatac gcgaaaatta cgccgccatg agtattcaga ccaatgaatg gaatgtactc
     6001 acccagacga tcaaacaacg catacaacgc gaccagtgaa agtactgccg ggaacatctg
     6061 gaaaatcagc attcctttca gcagcgtcgc tttgcctgga aagcgcatac gggcgaaagc
     6121 gtaggcgcag gtggtggaga gcgccacaat gccaatcgcg gaaatcccgg cgacctttac
     6181 cgagttccac agccacagca gtaccgggaa tggcggtggc gtaatgcgac catcagcctg
     6241 ttcaacgcta aaacctaacg ccagtttcca gtgatcccag gagatttgct ccgggatcag
     6301 gctaccggtc gcgaagtttc cctgacgcag cgagatagcg acgaccatca gcagcgggaa
     6361 cataatcgcc gcgataaaaa gtagcagtag caggtgagta ataaacaaac gtgctttttg
     6421 cgatttcggt tggaccattg ccatttttgt tatctccctt aatcaaactt cattcgcgtg
     6481 gctttcaggt tcactatcgc cagcgcaccc accagcagga agatcagcgt ggcaattgct
     6541 gccgccagac cgaagtcctg acccccgccg ccttcaaaag cgatacggta ggtgtagtta
     6601 acaagcaggt cggtataacc ggctggcgtg gtcgtgccaa gacgatccgg gccgccgttg
     6661 gttaacagct gaatcagcac gaagttgtta aagttaaagg cgaagctggc gatcatcagc
     6721 ggcgtcagcg gtttaatcag cagcggcagc gtaatcttaa agaagttctg gaacggacct
     6781 gcgccatcca ttgctgaggc ttcatacaaa tcgtccggaa tcgctttcag caagcccatg
     6841 cagaggatca tcatgtacgg ataacccagc caggtattga cgataatcag catcgtgcgg
     6901 gcggtggtcg gatcgctgaa ccaggcgggc ttcacgccaa acagcgcgct caacatcatg
     6961 ttgatttcac cgaagctctg gttaaacaat cctttgaaaa tcaagattga aatgaacgat
     7021 ggcaccgcgt agggcagaat cagcaggacg cgatagaccg ctttgccgcg caacgcttcc
     7081 cactgcacca gacatgccag aaccatgccg accgccaccg ttaaaaagac agtgatcagc
     7141 gagaacacca cggtccagac gaaaatggcg aggaacggtt tctgaatgcc ttcgtcggta
     7201 aagacgcggg taaagttttt ccagccggtg gtcacggtgt aaccggggct tagcttttca
     7261 tcaccccagt tgccgtcggc ggtaatggac tggtaaaagc caatttggtt attcggacga
     7321 tatttcacgc cgctctgatt attcgtcaac gtgccgtcac cgtcgagtgt gtagagcggc
     7381 tgcgtgccag aaaactggcg cagggagctc atcatcactt tgttgccatc cggcagaatg
     7441 gcggtaatgt cactcagcgc ctgacgattc tgggtaatca cgcgcagatt cgcgcgttcg
     7501 ccttcgggct gggcggtcgt ttctttcagt tgcagttttt gctcgccgcc aaatttaaac
     7561 gcgtcggaga ggtaattttt gccggtttcg ccgtcgctga gcgccagttg ccactcatcg
     7621 cccgccgggt aaagaccaaa gttataggtt ttgcctgctt gccaggagcg atctaacaac
     7681 acttcctgcg cacgttcaaa agtcagctgg ttagtgctgc tgtagttggt gaaggcaatg
     7741 gcgatggtgc agaccagagg gaagaggacg aataatccca ttccagccat tcccgggtaa
     7801 acatagcgcc aggcgtaggc tttacgattg gcgaaaatat acagccccgc tgaactcaat
     7861 atcagcgtgg taatggcgaa caggtattcc ccttgtgcgt acattaaaac aacaaggtaa
     7921 cccaccagca ggccgagcag acctggcact gaccatttca gcgcgtcgct ttgccaccaa
     7981 tgtttctttt taatgacatc catggggttc ttcctcattc caggacggat aaggctttca
     8041 cgccttatcc gacaacaact gcctgatgcg acactgatgc gtcttatcag gcatacatgc
     8101 gtttcagttt tgtaggccgg acaaggcgtt cacgccgcat ccggcatttc acagcattac
     8161 ttggtgatac gagtctgcgc gtctttcagg gcttcatcga cagtctgacg accgctggcg
     8221 gcgttgatca ccgcagtacg cacggcatac cagaaagcgg acatctgcgg gatgttcggc
     8281 atgatttcac ctttctgggc gttttccatg gtggcggcaa tacgtggatc tttcgccaac
     8341 tcttcctcgt aagacttcag cgccactgca cccagcggtt tgtctttatt gaccgcttcc
     8401 agaccttcgt cagtcagcag atagttttcg aggaactctt tcgccagctc tttgttcgga
     8461 ctggcggcgt taataccggc gctcagcacg ccaacgaacg gtttagatgg ttgacccttg
     8521 aaggtcggca gtaccgttac accataattc actttgctgg tgtcgatgtt ggaccatgcc
     8581 cacgggccgt tgatggtcat cgctgtttcg cctttattaa aggcagcttc tgcgatggag
     8641 taatcggtgt ctgcattcat gtgtttgttt ttaataaggt caaccaggaa ggtcagaccc
     8701 gctttcgcgc cagcgttatc cacgcccacg tctttaatat cgtacttgcc gttttcatac
     8761 ttgaacgcat aacccccgtc agcagcaatc agcggccagg tgaagtacgg ttcttgcagg
     8821 ttgaacatca gcgcgctctt acctttcgct ttcagttctt tatccagcgc cgggatctct
     8881 tcccaggttt ttggtgggtt cggcagcaga tctttgttat aaatcagcga taacgcttca
     8941 acagcgatcg ggtaagcaat cagcttgccg ttgtaacgta cggcatccca ggtaaacgga
     9001 tacagcttgt cctggaacgc tttgtccggg gtgatttcag ccaacaggcc agattgagcg
     9061 tagccaccaa agcggtcgtg tgcccagaag ataatgtcag ggccatcgcc agttgccgca
     9121 acctgaggga atttctcttc cagtttatcc ggatgctcaa cggtgacttt aattccggta
     9181 tctttctcga atttcttacc gacttcagcg agaccgttat agcctttatc gccgttaatc
     9241 cagattacca gtttaccttc ttcgattttg gcgagagccg aggcggaaaa catcatcgtc
     9301 gttaatgcgg ataatgcgag gatgcgtgca cctgttttta ttttcataat ctatggtcct
     9361 tgttggtgaa gtgctcgtga aaacacctaa acggactcta gtttctttat acggcaacct
     9421 ctttccatcc tccttgcccc tacgccccac cgttgctttg tgtgatctct gttacagaat
     9481 tggcggtaat gtggagatgc gcacataaaa tcgccacgat ttttgcaagc aacatcacga
     9541 aattccttac atgacctcgg tttagttcac agaagccgtg ttctcatcct cccgcctcct
     9601 cccccataaa aaagccaggg ggtggaggat ttaagccatc tcctgatgac gcatagtcag
     9661 cccatcatga atgttgctgt cgatgacagg ttgttacaaa gggagaaggg catggcgagc
     9721 gtacagctgc aaaatgtaac gaaagcctgg ggcgaggtcg tggtatcgaa agatatcaat
     9781 ctcgatatcc atgaaggtga attcgtggtg tttgtcggac cgtctggctg cggtaaatcg
     9841 actttactgc gcatgattgc cgggcttgag acgatcacca gcggcgacct gttcatcggt
     9901 gagaaacgga tgaatgacac accgccagca gaacgcggcg ttggtatggt gtttcagtct
     9961 tacgcgctct atccccacct gtcagtagca gaaaacatgt catttggcct gaaactggct
    10021 ggcgcaaaaa aagaggtgat taaccaacgc gttaaccagg tggcggaagt gctgcaactg
    10081 gcgcatttgc tggatcgcaa accgaaagcg ctctctggcg gtcagcgtca gcgtgtggcg
    10141 attggccgta cgctggtggc cgagccaagc gtatttttgc tcgatgaacc gctctccaac
    10201 ctcgatgctg cactgcgtgt gcaaatgcgt atcgaaatct cccgtctgca taaacgcctg
    10261 ggccgcacaa tgatttacgt cacccacgat cagctcgaag cgatgacgct ggccgacaaa
    10321 atcgtggtgc tggacgccgg tcgcgtggcg caggttggga aaccgctgga gctgtaccac
    10381 tatccggcag accgttttgt cgccggattt atcggttcgc caaagatgaa cttcctgccg
    10441 gtaaaagtga ccgccaccgc aatcgatcaa gtgcaggtgg agctgccgat gccaaatcgt
    10501 cagcaagtct ggctgccagt tgaaagccgt gatgtccagg ttggagccaa tatgtcgctg
    10561 ggtattcgcc cggaacatct actgccgagt gatatcgctg acgtcatcct tgagggtgaa
    10621 gttcaggtcg tcgagcaact cggcaacgaa acccaaatcc atatccagat cccttccatt
    10681 cgtcaaaacc tggtgtaccg ccagaacgac gtggtgttgg tagaagaagg tgccacattc
    10741 gctatcggcc tgccgccaga gcgttgccat ctgttccgtg aggatggcac tgcatgtcgt
    10801 cgactgcata aggagccggg cgtttaagca ccccacaaaa cacacaaagc ctgtcacagg
    10861 tgatgtgaaa aaagaaaagc aatgactcag gagatagaat gatgattact ctgcgcaaac
    10921 ttcctctggc ggttgccgtc gcagcgggcg taatgtctgc tcaggcaatg gctgttgatt
    10981 tccacggcta tgcacgttcc ggtattggct ggacaggtag cggcggtgaa caacagtgtt
    11041 tccagactac cggtgctcaa agtaaatacc gtcttggcaa cgaatgtgaa acttatgctg
    11101 aattaaaatt gggtcaggaa gtgtggaaag agggcgataa gagcttctat ttcgacacta
    11161 atgtggccta ttccgtcgca caacaaaatg actgggaagc taccgatccg gccttccgtg
    11221 aagcaaacgt gcagggtaaa aatctgatcg aatggctgcc aggctccacc atctgggctg
    11281 gtaagcgctt ctaccaacgt catgacgttc atatgatcga cttctactac tgggatattt
    11341 ctggtcctgg tgccggtctg gaaaacatcg atgttggctt cggtaaactc tctctggcag
    11401 taacccgctc ctctgaagca ggtggttctt cctctttcgc cagcaacaat atttatgact
    11461 ataccaacga aaccgcgaac gacgttttcg atgtgcgttt agcgcagatg gaaatcaacc
    11521 cgggcggcac attagaactg ggtgtcgact acggtcgtgc caacctgcgt gataactatc
    11581 gtctggttga tggcgcatcg aaagacggct ggttattcac tgctgaacat actcagagtg
    11641 tcctgaaggg ctttaacaag tttgttgttc agtacgctac tgactcgatg acctcgcagg
    11701 gtaaaggtct gtcgcagggt tctggtgttg catatgttga cgaaaaattt tcctatgaca
    11761 ttaacaacaa cggtcacatg ctgcgtatcc tcgaccacgg tgcgatctcc atgggcgaca
    11821 actgggacat gatgtacgtg ggtatgtacc aggatatcaa ctgggataac gacaacggca
    11881 ccaagtggtg gaccgtcggt attcgcccga tgtataagtg gacgccgatc atgagtaccc
    11941 tgatggaaat cggctacgac aacgttaaat ctcaacgtac tggtgatacc aacaaccagt
    12001 acaaaatcac acttgctcaa caatggcagg ctggcgacag catctggtcg cgtccggcta
    12061 ttcgtgtttt tgccacctac gccaagtggg atgagaaatg gggttacgat tacaccgaca
    12121 aagctaaagg tactccgaac tacggcaaag ccgttccagc taacttcgaa ggcggcagct
    12181 tcggtcgtgg cgacagcgac gagtggacct tcggtgccca gatggaaatc tggtggtaat
    12241 agcaaaacct gggccggata aggtgcttgc gccgcatccg gcatccaacg cctgatgcga
    12301 cgcttgcgcg tcttatcagg cctacaacgg ctgtcaaatg taggccggat aaggcgttta
    12361 cgccgcatcc ggcataaaaa caggttgtca ttatctgaaa ggggcgcaag cccctctgat
    12421 tatcgggttt agcgcgctat tgcctggcta ccgctgagct ccagattttg aggtgaaaac
    12481 aatgaaaatg aataaaagtc tcatcgccct ctgtttatca gcagggttac tggcaagcgc
    12541 gcctggaatc agccttgccg atgttaacta cgtaccgcaa aacaccagcg acgcgccagc
    12601 cattccatct gccgcgctgc aacaactcac ctggacacca gtcgatcaat ctaaaaccca
    12661 gaccacccaa ctggcgaccg gcggccaaca actgaacgtt cccggcatca gtggtccggt
    12721 tgctgcgtac agcgtcccgg caaacattgg cgaactgacc ctgacgctga ccagcgaagt
    12781 gaacaaacaa accagcgtat ttgcgccgaa cgtgctgatt cttgatcaga acatgacccc
    12841 atcagccttc ttccccagca gttatttcac ctaccaggaa ccaggcgtga tgagtgcaga
    12901 tcggctggaa ggcgttatgc gcctgacacc ggcgttgggg cagcaaaaac tttatgttct
    12961 ggtctttacc acggaaaaag atctccagca gacgacccaa ctgctcgacc cggctaaagc
    13021 ctacgccaaa ggcgtgggta actcgatccc ggatattccc gatccggttg ctcgtcatac
    13081 cagcgatggc ttactgaaac tgaaagtcaa aacgaactcc agctccagcg tgttggtagg
    13141 accactattt ggttcttccg ctccagctcc ggttacggta ggcaacacgg cggcaccagc
    13201 tgtggctgca tccgctccgg caccggtgaa gaaaagcgag ccgatgctca acgacacgga
    13261 aagttatttt aataccgcga tcaaaaacgc tgtcgcgaaa ggtgatgttg ataaggcgtt
    13321 aaaactgctt gatgaagctg aacgcctggg atcgacatct gcccgttcca cctttatcag
    13381 cagtgtaaaa ggcaaggggt aattacgccc cacagtgctg attttgcaac aactggtgcg
    13441 tctcctggcg cacctttttt tatgcttcct tcttgggata tgagcgattt tttatagtaa
    13501 ctcacttctt cttcactaag aatgtccatt atctcaatgc cttatcagag attcttttcc
    13561 tttcgccggt agtgtctgga cattcaggct acttttccag gttattttat ttctgttatg
    13621 cagaggtttt atgataagtc atatcccaaa ttctggcggc aataactctt tgatgaaaca
    13681 tga
//
LOCUS       DAZFXT010000121        13550 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.121, whole genome shotgun sequence.
ACCESSION   DAZFXT010000121 DAZFXT010000000
VERSION     DAZFXT010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.121"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..686
                     /locus_tag="ACHKW0_003846"
     CDS             <1..686
                     /locus_tag="ACHKW0_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HFO7476601.1"
                     /translation="LDDSYFAHVKMTDGTLNACSAMHFQFYNAAMNRANIKNTFLDYS
                     NFYMAYMAEVNLYKVIAPYVNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLI
                     DSKLFCTWLTNTFLEMVICTGSNMAKVDFNDANLSNCHFNCSILTKACMFNTRLYRVN
                     FDEASVQGMGISILRGEENIPIDSDTLVTLQKFFEEDCTSHTGMSQTEDNINAAAMKI
                     TADIMQHAD"
     gene            complement(839..2488)
                     /gene="yjcE"
                     /locus_tag="ACHKW0_003847"
     CDS             complement(839..2488)
                     /gene="yjcE"
                     /locus_tag="ACHKW0_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HFO7476602.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2639..3988)
                     /gene="ghxP"
                     /locus_tag="ACHKW0_003848"
                     /gene_synonym="yjcD"
     CDS             complement(2639..3988)
                     /gene="ghxP"
                     /locus_tag="ACHKW0_003848"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HFO7476603.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4534..4998)
                     /gene="soxR"
                     /locus_tag="ACHKW0_003849"
     CDS             complement(4534..4998)
                     /gene="soxR"
                     /locus_tag="ACHKW0_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HFO7476604.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5084..5407
                     /gene="soxS"
                     /locus_tag="ACHKW0_003850"
     CDS             5084..5407
                     /gene="soxS"
                     /locus_tag="ACHKW0_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HFO7476605.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5410..6996)
                     /gene="pdeC"
                     /locus_tag="ACHKW0_003851"
     CDS             complement(5410..6996)
                     /gene="pdeC"
                     /locus_tag="ACHKW0_003851"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HFO7476606.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7426..7707
                     /gene="yjcB"
                     /locus_tag="ACHKW0_003852"
     CDS             7426..7707
                     /gene="yjcB"
                     /locus_tag="ACHKW0_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HFO7476607.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7806..8342)
                     /gene="ssb1"
                     /locus_tag="ACHKW0_003853"
     CDS             complement(7806..8342)
                     /gene="ssb1"
                     /locus_tag="ACHKW0_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HFO7476608.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8596..11418
                     /gene="uvrA"
                     /locus_tag="ACHKW0_003854"
     CDS             8596..11418
                     /gene="uvrA"
                     /locus_tag="ACHKW0_003854"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HFO7476609.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11453..11809)
                     /gene="yjbR"
                     /locus_tag="ACHKW0_003855"
     CDS             complement(11453..11809)
                     /gene="yjbR"
                     /locus_tag="ACHKW0_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HFO7476610.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11813..12229)
                     /gene="yjbQ"
                     /locus_tag="ACHKW0_003856"
     CDS             complement(11813..12229)
                     /gene="yjbQ"
                     /locus_tag="ACHKW0_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HFO7476611.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12340..13053)
                     /gene="aphA"
                     /locus_tag="ACHKW0_003857"
     CDS             complement(12340..13053)
                     /gene="aphA"
                     /locus_tag="ACHKW0_003857"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HFO7476612.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            13455..>13550
                     /locus_tag="ACHKW0_003858"
     CDS             13455..>13550
                     /locus_tag="ACHKW0_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476613.1"
                     /translation="MNISYVNSNKTTSLPVELDALNNKDISYAKDF"
BASE COUNT         3553 a         3511 c         3269 g         3217 t
ORIGIN      
        1 ttctcgatga ctcatatttc gctcatgtaa aaatgactga cgggactcta aatgcatgct
       61 ccgctatgca ttttcaattc tacaatgcag caatgaatag agccaatatt aaaaatacct
      121 tccttgacta ttcaaatttt tatatggcgt acatggctga ggtaaatctt tataaagtaa
      181 tagcgccata tgttaattta tttaaagccg accttagttt ctctaaactc gatttaatta
      241 actttgaaca tgctgatctg tctcgcgtca atctgaacaa agcaatcctc cagaatataa
      301 acttaattga tagcaaactc ttttgtacgt ggctgacaaa tacattcctc gaaatggtta
      361 tatgtaccgg ctctaatatg gctaaggttg attttaatga tgccaattta agcaactgcc
      421 atttcaactg ttctatttta acaaaagcct gtatgtttaa tacccgtctc tatcgggtta
      481 atttcgatga ggctagcgtc caaggaatgg gcatttccat tctccgtggg gaggaaaata
      541 tccccattga tagtgatacc ctggtaacac tacagaaatt ctttgaagaa gattgtacct
      601 ctcatactgg catgtcacaa actgaggata atattaatgc agccgctatg aagattactg
      661 cagatattat gcaacacgca gattgaggtt aagtcgaaga acagccaatg aattgacttt
      721 acaagaggat aacttatttg ttttagaatc atgaatgtta gcccgagcta acctgttaaa
      781 ggtgcaaaag attgcacctt tttcagatta cctcgggcct ggttacgtcg ctttcggctt
      841 actgattttc ctcaattagc aacgcttcaa gcaaatcgag atcgtgcagt aatttttgca
      901 gcgtttcgtt gctgatctcc cgcgtggcgc gcaggtggta cagttcagca cgttcagaac
      961 gcaatgccgc cagacggaag cgacgctcaa ggttctcttc ctgcacggaa ctttcaacgt
     1021 cgttacgtcc atcggcgcga cgacgcaggt taccaatgac gcgagaactg acctccgtaa
     1081 gcagctggtt atcgatattc tcttcggtat ccgccgccag acgctcctcc attttctgga
     1141 tcgccacaat cgccacttct gccgttgccg ctcgcgcaat acgttcttct ttcaattgtt
     1201 gcgaatggtc cgcgacttca atgtgttgta gcagaatagg caacatcacc acgccgacaa
     1261 acagcgagaa gagaatgacg ccagccgcca ggaacaccag ctcatagcgc gccgggaaga
     1321 cgttaccatc cggcaagagc agcgggattg agagcacacc ggccagagtg atcgccccac
     1381 gcaccccggc gaaagacgcg atcaggattt ctcgtgtggt ccacgaacca aactccatcg
     1441 gcttcttctt caggaagcgg ttgctgaact ttttcatcgt ccacagccag ccgaaacgga
     1501 ccagcatcag cgccgcatat atcagaataa tatcggtaaa cagcatccag atttcgacgt
     1561 tagggtcgat ttctgctgcc atcagcgacg tctccagaat acccggcagc tgcagaccta
     1621 acagcaggaa caccatgccg ttaaagacaa attccagcat cgcccaggtg ctgtttgcac
     1681 gcaggcgcat tgccagcggc gcacggcgca tcacaccgga gcgggtgatg gtcatcccgg
     1741 cggcaaccgc agcgaggatg ccggaaacgc caatatgttc ggcaatcaga taagaagcga
     1801 atggcagcaa gaacagcaga acaatctgcg tcgcgggttc atcaccgccc cagcggctga
     1861 ggaatcgcag cgagcgacca tacagccagc tcaccacaaa accggcaaga ataccgccaa
     1921 tggctacttt cataaattcg accgtcgcac cgccgacggt gaagatcatc gtccccatcg
     1981 ccactgccac ggcaaacttc aacgacacca ggccggaagc gtcattcatc aacgcttcgc
     2041 cctgcaaaat gcccatgatt tttttcggga tgcgcccttc gccaacaatc ccggagagcg
     2101 ccacagcatc ggtcggagaa agtaccgccg ccagcgcaaa ggcggggatc agcggaatgc
     2161 ccggcaccac ccagtaaata aggaagccaa tgccgaccac ggtgaccacc accagcgcca
     2221 gcgcgaggcc gaaaatctct cgaccatgtt cgagaaattc acgggtcggc gttttccagc
     2281 catcagcgaa cagcaacggc gggataaaca agactaaaaa gagttcagga tcaaactcca
     2341 catgcaaacc aaacgtcggc cacgccagta gcgcaccgat ggcgatttgc ataagcggaa
     2401 gcgggatctg aaagggcatg acacgagtga ccaccccgga cagcgagacc acgagggtca
     2461 ttatcagtat ggtgaagaag atttccatgc gttccctgtt tgcgatgttt tttatgatct
     2521 gagaaagacg ttatgcctga attctcattt tttcagaata tcgcaaaagc tcacacaatg
     2581 tgcggagaaa ataaaaacgg gcagccattg ccacccgttt tcagaatccg atgaaagatt
     2641 agatagccca gccacccgca tagaaggtca ccagcgccac ggcgataacg accgtaccga
     2701 tgttcaactt gcgccattcg ccggaaacca gacgaccaat caccagagtc gcgaagccga
     2761 tcatgatgcc tgttacgatg ttacaggtca gcacgatgaa taccgccgta accagacccg
     2821 ccatcgcatc aacaaaatca gcaaagtcga ttttcgccac gttgctcagc atcagcaggc
     2881 caacgtacat cagcgccgga gccgttgcat accccggaac gaggtaagag agcggagaga
     2941 ggaacagaat cagcaggaac agcacgccaa cggtgatagc cgtcaaaccg gttttaccgc
     3001 ccgccgccgt acccgccgca gactcgatgt ataccgctgc cggagctgca cccaccaggc
     3061 cagagaaaac gctgctcatg gagtcagtgg tcagtgcttt cccaccgtcg ataatttgcc
     3121 catctttatc cagcaggttc gcctggccgg cgacggcacg gatagttccg gtggcatcaa
     3181 atactgccgt cattaccagc gccagaacgc ttggcaggac tacaggattc agcgcgccca
     3241 taatgtccag gctgccaatc agtgagttgc cgttttcatc gctcaatgaa ggcatggcga
     3301 aaacgccgga gaaatggacg ttaggatcga agatcaaacc gacaattgag ataccgataa
     3361 tggtcagcag aatgccacca gggactttca gtttttccag gccgatgatc accgccagac
     3421 ctaccagtga cataatcacc gggaaggtcg cgaaatcacc cagcgcaacg ggcagaccat
     3481 caagcgggtt tttaatcacc agaccgacac cgttagcggc aatgagcagc aggaacagac
     3541 cgataccaat ccccgtgccg tgcgccacac cgtgcggcaa gttgcgcaaa atccagctac
     3601 ggatacccgt ggcagaaatt accgtaaaca gcacacccat caggaacacg gcacccagcg
     3661 cgacaggtac gctaatatgt tgccccagca ccaggctgaa tgcggtaaac gccgtcaggg
     3721 agatggcgca accaatcgcc aacggcagat tagcccacag acccatcacg atagaaccga
     3781 gtccggcaac cagacaggtt gcaacgaaaa ctgccgcagg cgggaagcct gctttaccca
     3841 acatacctgg aacgacgatg accgagtaga ccatcgccag aaacgttgtt aacccggcaa
     3901 ccacttcctg acggacagtg cttccacgtt gtgaaatttt aaaccaggcg tcgagtgaac
     3961 cgccggtacg cgctgatggc gtagacataa gaaaacatcc cctgagagtt taattttcgt
     4021 caagatgcgt ggcggacaat cccctgccag cgaaacgtta tcttcctgct ccaggtttgc
     4081 gacaatttac tgcgtcgcaa aaaagcaaac gtttagctcc gcgcatacaa aacgggtggt
     4141 aaaaaaagca aacgattatc cggcgatcat agcggctttt tcaaccgaag tttgctgctt
     4201 ttttctcttt tattgtcttg acgatgaaaa accaggcaat tgatgcgcaa acgttatcgt
     4261 cggtgcgcaa gattgcagcg ttatcgagcc gtataccgtt caggattgcg ataaaaaatc
     4321 gtctgagatg acaaaaatat tgcggataaa aagtatactt ttacactggc gaagaaatgc
     4381 cggtgacata caattaaagc atcaacacca accggaacct ccaccacgtg ctcgaatggg
     4441 gtgtgttgac gtcgggggaa accctcctgt gtaccagcgg gatagagaga aagacaaaga
     4501 ccggaaaaca aactaaagcg cccttgtggc gctttagttt tgttcatctt ccagcaagcg
     4561 tgcgccggta ccttcttctc ctaagcggtc gcccgggtta cgcaacgggc aatcactgcg
     4621 cgaaaggcag ccacaaccaa tacatccgtc cagttcgtca cgcagcgcta ctaaggtatg
     4681 aatgcgccga tccaactctt ctcgccattg ggacgaaagc tgtttccact ctttcgcact
     4741 taacgtatgc ccttcgggca acacgccaaa cgcttcacca atggtcgcca gcggaatgcc
     4801 aatacgctga gcaattttga taattgcaac atatcgcaac acatcacgtt tatatcgccg
     4861 ctgattgccg ctgttacgga tactggtaat caacccttta ctttcataga aatgcagcgc
     4921 cgataccgcc acaccgctgc gtttcgccac ttcgccgggg gttagcagcg ctttaatgcg
     4981 gggtaatttc ttttccataa atcgctttac ctcaagttaa cttgaggaat tatactcccc
     5041 aacagatgaa ttaacgaact gaacactgaa aagaggcaga tttatgtccc atcagaaaat
     5101 tattcaggat cttatcgcat ggattgacga gcatattgac cagccgctta acattgatgt
     5161 agtcgcaaaa aaatcaggct attcaaagtg gtacttgcaa cgaatgttcc gcacggtgac
     5221 gcatcagacg cttggcgatt acattcgcca acgtcgcctg ttactggccg ccgttgagtt
     5281 gcgcaccacc gagcgtccga tttttgatat cgcaatggac ctgggttatg tctcgcagca
     5341 gaccttctcc cgcgttttcc gtcggcagtt tgatcgcact cccagcgatt atcgccaccg
     5401 cctgtagttt tattgcccgc gcgttaactc ccgcgcgggt aattgctcca tccattgcat
     5461 aaacacctgc ggcggcatcg ccttcgcaaa aaaccatccc tggcaatagc gcacgccgcg
     5521 tttgcgcagc cagttaacct gctcctcagt ttcgacgcct tcagcgatcg tttttaaccc
     5581 caggctgtgc gccagctcga tgatgtgttc cgcaatcaaa tgactggttt tgtgggtggt
     5641 cagcgtttca acaaacgatt tatcgatttt caaaatatcg acattcaatg atttaaggtt
     5701 atgcaagtta gagtagccaa taccaaaatc atcaattgcc acttcgtaac cagcctggcg
     5761 gaaagccaga ataatcggcg tcattttttc gacatcaaga aatgcatgct cagtcacttc
     5821 aaatttaatt tgctgcggac gcaccgcgta ttgctctgtt ttctgattga ttcgcgctat
     5881 caaccgtgac gtatgaaaat cggaggccga caggttaata gaaacatagc gatcggcatg
     5941 tgttgccaag tacgcgccca gatcgcggaa gacattatca ataacataat cagttatctg
     6001 ttctatcatc ccctcctttt ctgccagcgg aataaactct gccggattca ttatttgccc
     6061 ctgctcacca agccaacgta acaacgcttc agcgccgata catttttctg ttttgatatc
     6121 gattattggc tggtaataaa gacaaagttg atgtttttcg agggcgcgtt gcaatttacg
     6181 tttgggagat aaatagtttt gtcgaatacg tagccagagt aatagcagaa caagactccc
     6241 caaaataccg gcgggcaacg caaaaataag atgattataa aaatgggtta taagacgttg
     6301 atatgaagtc gcaacaatgg ctgcaattgg gcgttttgtc gaataaactg tcgcatataa
     6361 atagccattt ctttgtacgg ttaaatcctt caaatgaatc agcggagaaa acgttgctgc
     6421 cgaggcctct ttgcttaacg agaaaaaggt tttcgtcacc gtatcataca caccccattg
     6481 caatgtcggg tcatcagaca tcacttcact ccagaagaga gggttgataa ccgccacata
     6541 atttccccgc tgcatatagg tcattttata gccagagaaa aaaggcgtat cgcggtaata
     6601 atagatagaa acgttaggtt cacgcttata atcggccggt gcaatcgtat agccgtttac
     6661 aggcgctatc agcgatgagc ataaaaaatg gttatcacgg gcatagatca attcattaat
     6721 ataaagatag ccacgaataa tattcaacat tcgcttttga tgggctggag tgcaaacttg
     6781 cccctgatag cgttcagctg catcgctcac taaatctgcc tggcgaatga ccagctcgga
     6841 tttatccagt gccagttgag caaaggttcg gagctgatta ttcacttctg attttgccca
     6901 aaggaaggca atccatagcg aaagaatgat gggaaagaga actaaaaaga taatgcccgg
     6961 caacgccagt aattggtgtc gtgcacgatg actcattgct atcaatttcc tgcctgttta
     7021 tacccggcca ttgcaaggtg aaaaccctgg gtaccgacgt ctttagataa atcttacggt
     7081 ataaatagca cttttgcctg atttgaaatt gatacctttc taatcgtcac ctggatgaaa
     7141 ataattcatt gatattcaaa attaatttcc tacaggaatg cagaggcggc gggaagatat
     7201 gcccttaatg ccactgaaaa accacataat ctgtacagca ataaaagtca cggcctaata
     7261 tggctaacaa acaatcaatt gtgatatagt tcacaaaatt aatgaaacaa acagagtgtt
     7321 tcatttttgt gttctgcaag ccccgcgata gccagagtgt accacgcctc ccgtgaacaa
     7381 cgccgcgctg tccagggcat cggctctttt cagaggaatt gtttgatggc taccctcacc
     7441 actggcgtgg ttcttcttcg ctggcaactt cttagtgccg taatgatgtt tctggccagc
     7501 acactcaaca tccgttttcg tcggtctgat tatgtcgggc ttgcggtgat cagcagcggt
     7561 ctgggcgttg tttccgcctg ttggttcgca atggggttgc ttggcatcac aatggcggac
     7621 atcaccgcta tctggcacaa catcgagtcg gtgatgatag aagagatgaa tcagacaccg
     7681 ccacaatggc caatgatttt gacctgatac gtagaagcct ccataacgga ggcttctttt
     7741 ttatggctgg cagatgcttt aatcatccac cttaaaacaa tataacctat tgttttaatg
     7801 acaaatcaga acggaatgtc atcatcaaag tccatcggcg gctcgttaga cggcgctgcc
     7861 ggagcggact gctgcgggcg agactgcgcg ccgccgctga actgattgcc accctgcggc
     7921 tgctgaggct gaccccaacc gccctgcggc tgaccaccac cgatattacc acctgccgga
     7981 gcgccaccac cctgacgacc acccagcatc tgcatggtgc cgccaacgtt caccacgact
     8041 tctgtggtgt agcgatcctg gccggattga tcggtccatt tgcgggtacg cagctgacct
     8101 tcgatataaa cctgagaacc tttacgcaga tattcgctgg ccacttctgc cagtttgccg
     8161 aacagcacaa cgcggtgcca ttcagtctgt tctttcatct cgccggtcgc tttatcacgc
     8221 caggattcgg aagtagccag cgtaatgttg gcaactgcgc caccatttgg catgtagcgt
     8281 acttccgggt cctgacccag attaccaacg agaataacct tgtttacgcc tctgctggcc
     8341 atgttcgtgt ctcctgaaaa aaatcgttct gaataagtgt aaacgcgcga ttgtaccatt
     8401 accaatagcg cttttactat gttgtgacct cggttccggg aaacaaacct ggccagacat
     8461 tgttacacaa cactccggat aatgcattcc aatactgtat attcattcag gtcaatttgt
     8521 gtcataatta accgtttgtg atcgccggta gcaccatgcc accgggcaaa aaagcgttta
     8581 atccgggaaa ggtgaatgga taagatcgaa gttcggggcg cccgcaccca taatctcaaa
     8641 aacatcaacc tcgttatccc ccgcgacaag ctcattgtcg tgaccgggct ttcgggttct
     8701 ggcaaatcct cgctcgcttt cgacacctta tatgccgaag ggcagcgccg ttacgttgaa
     8761 tccctttccg cctacgcgcg gcagtttctg tcactgatgg aaaagccgga cgtcgatcat
     8821 attgaggggc tttctcctgc catctcaatt gagcagaaat cgacgtctca taacccgcgt
     8881 tctacggtgg ggacaatcac cgaaatccac gactatttgc gtttgttgtt cgcccgcgtc
     8941 ggcgaaccgc gctgtccgga ccacgacgtc ccgctggcgg cgcaaaccgt cagccagatg
     9001 gtggataacg tgctgtcgca gccggaaggc aagcgtctga tgctactcgc gccaatcatt
     9061 aaagagcgca aaggcgaaca caccaaaacg ctggagaacc tggcaagcca gggttacatc
     9121 cgtgctcgta ttgatggcga agtctgcgat ctttccgatc cgccgaaact ggaactgcaa
     9181 aagaaacaca ctattgaagt ggtggttgat cgcttcaagg tgcgtgacga tcttacccaa
     9241 cgtcttgcgg agtcgtttga aaccgcgctg gagctttccg gtggtacagc ggtagtggcg
     9301 gatatggacg acccgaaagc ggaagagctg ctgttctccg ccaacttcgc ctgcccaatt
     9361 tgcggctaca gtatgcgtga actggagccg cgactgtttt cgtttaacaa cccggcaggt
     9421 gcctgcccaa cctgtgacgg ccttggcgta cagcaatatt tcgatcctga ccgcgtgatc
     9481 caaaaccccg agctgtcact ggctggcggt gcgatccgtg gctgggatcg ccgcaacttc
     9541 tattacttcc agatgctgaa atcgctggca gatcactata agttcgacgt cgaagcgccg
     9601 tggggcagcc tgagcgcgaa cgtgcataaa gtggtgttgt acggttctgg caaagaaaac
     9661 attgaattca aatacatgaa cgatcgtggc gatacctcca tccgtcgtca tccgttcgaa
     9721 ggcgtgctgc acaatatgga gcgccgttat aaagagacag aatccagtgc ggtacgtgaa
     9781 gaattagcca agtttatcag caatcgccca tgcgccagct gcgaagggac gcgtctgcgt
     9841 cgggaagcgc gccacgttta tgtcgagaat acgccgctgc ctgctatctc cgacatgagc
     9901 atcggtcatg cgatggaatt cttcaacaat ctcaaactag ctggtcagcg ggcgaagatt
     9961 gcagaaaaaa tccttaaaga gatcggcgat cgtctgaaat tcctcgttaa cgtcggcctg
    10021 aattacctga cactttcccg ctcggcagaa acactttccg gcggtgaagc ccagcgtatc
    10081 cgtctggcga gccagattgg tgcgggcctg gttggcgtta tgtatgtact ggacgagccg
    10141 tctatcggcc tgcaccagcg cgataacgag cgcctgttgg gtacgcttat ccatctgcgc
    10201 gatctcggta ataccgtgat tgtggtggag catgacgaag acgcaattcg cgccgctgac
    10261 catgtgatcg atatcggtcc tggtgcgggt gttcacggcg gtgaagtggt cgcggaaggt
    10321 ccactggaag cgattatggc ggtgccggag tcgttaaccg gacagtacat gagcggtaaa
    10381 cgcaagattg aagtgccgaa gaaacgcgtt ccggcaaatc cggaaaaagt gctgaagctg
    10441 acaggcgcac gcggcaacaa cctgaaggac gtgacgctca cgctgccagt cggtctgttt
    10501 acctgcatca caggggtttc aggttccggt aaatcgacgc tgattaacga cacactgttc
    10561 ccgattgccc aacgccagtt gaatggtgcg accatcgccg aaccggcacc gtatcgcgat
    10621 attcaggggc tggagcattt cgataaagtg atcgatatcg accaaagccc aattggtcgt
    10681 actccacgtt ctaacccggc gacctatacc ggcgtgttta cgcctgtgcg cgaactgttt
    10741 gcgggcgtac cggaatcccg tgcgcgtggt tatacgccag gacgtttcag ctttaacgtc
    10801 cgtggcgggc gctgcgaagc ctgtcagggc gacggtgtga tcaaagtaga gatgcacttc
    10861 ctgccggata tctacgtgcc gtgcgatcag tgtaaaggta aacgctataa ccgtgaaacg
    10921 ctggaaatta agtacaaagg caaaaccatc cacgaagtgc tggatatgac catcgaagag
    10981 gcgcgtgagt tctttgatgc cgtacctgca ctggcgcgta agctgcaaac gttgatggac
    11041 gttggcctga cgtacattcg cctggggcag tccgcaacca cactttctgg tggtgaagcc
    11101 cagcgcgtga agctggcgcg tgagctgtca aaacgcggca ccgggcagac gctgtatatt
    11161 ctcgacgagc cgaccaccgg tctgcacttc gccgatattc agcaactgct cgacgtactg
    11221 cataaactgc gcgatcaggg caacaccatt gtggtgattg agcacaatct cgacgtgatc
    11281 aaaaccgctg actggattgt cgacctggga ccagaaggcg gcagtggcgg cggcgaaatc
    11341 ctcgtctccg gtacgccaga aaccgtcgcg gagtgcgaag catcacacac ggcacgcttc
    11401 ctcaagccga tgctgtaatc gttaaggccg ctttctgagc ggccttttcc tttcagagtt
    11461 gtaccagcaa tttacgcttt tcttccggta gtaaattcac tgcctgctga taagacgcat
    11521 ccaccagata atagatttgc gaatccggca gcgaaccgtc gagatacacg gtgctccagt
    11581 gcgctttatt cagatggcgg ctaggacgca catcgctgtg ctgctgacgt agcagctccg
    11641 ccagctccgg gctggttttc agcgaaacag ctgggcgatt ttctacttct ttcaccatcg
    11701 caaacagtac atcttccact ttgatctgcg tcgctttcca gtcgttatgc acgctctgtt
    11761 ctgcgcctgg ttttgccatg caatattgta gcaactccga aatggtcatt ttttactccc
    11821 cttgtagtgt cgcgatgatg cgacgcgatc cgccgtggat gcgatgttcc cccagccaga
    11881 tgccttgcca ggtgccggtc tgaatacgcc ctttatgtac cggcaataca agcgatgttc
    11941 ccagcattga ggatttgata tgagaaggca tatcgtctgc tccctcatag tcatgctcat
    12001 aatttccgtt gtcgggaacg gtgcggagga aaaaacgctc catgtcgtgg cgtacggtgg
    12061 gatcgcagtt ctcattaagt gtcagagagg cggaggtatg ttgcagcaac agatgcagta
    12121 agccgatgtt aacgcgcggc atatcagcca gctgattcag aatttcatcc gttaccagat
    12181 gaaacccacg agatttggcg ctaagcgtga gcgtcttttg ataccacatg tgctgctcct
    12241 tgataaaact ctcttaatca gtttgcagca agacagcgaa aggataaagg tgtgattaaa
    12301 aaaacagcat tcgggagagc gtcacgctct cccgctctgt cagtattctg aattgacgat
    12361 cacctcttca ccaaacgcac ccgcttgtgg caagggtttg taggtagagt tggaggcgcg
    12421 cagaatgcgg ataccacgag cgccgacatc gcgtgcggcg gtaatatcat tatcagaatc
    12481 gccataaaaa attcggatat ttttatcctg cagccattgc gattttgtat tttgccctgg
    12541 tttatcgccc gcaaagatca ccggattcat gttggtggca ggaatatgaa aattatccgc
    12601 cagcgttttt gaaaccgttt ctgttttcgt cggactacga ccagtcacaa agaagatcgc
    12661 gtcaccgcgg cgtacatgca tatcaatcag ctggcgagcg acctcttttg gaatgctgaa
    12721 ttcatcccag ccattgttca ttttttccca gaacacagga tttttcagat aatcttcgct
    12781 ttctggcgag aaggtttttt tgccgcgcca gaagcccgga ctggaaaaaa gtaccgtgtc
    12841 atcgatatca aaccccaccg ccattggcgg acgccctgcg aggctatttt caatttgtgc
    12901 gaccgaaacc caatgaatgg gtgcctgttc agcaagcctg gcaacgttag tcccagggtt
    12961 aagcggtgaa ggagatgagg ccagggcaac agcggaactg tttagagcaa acaataagca
    13021 aacggcactg attgcctgtg tgatcttgcg catatttttc cctaaatagt cagtatgttg
    13081 aaacttttta ttgtgagatt tgttgcaaaa accatctgac cataacgcca gctgccgggg
    13141 aaaggaagga ttatctgcgg tttttgtgat gaaaagcagg atgattatat ttaacccagt
    13201 aaatacgttc ctgtcttttt ttgtataaaa aatgtttatt actcgatgta aggatacatt
    13261 aattttacgt ttattatgat gaatcctcgg caaatgagcg aaatttgata gaacatttat
    13321 ctgctgtacg atttaatgaa cttaatgata cagcattaca aaaacaatcg aagtttataa
    13381 agatgatttc tgattgacca gccccctacc ctacaatggt gattaatttt attactcgcg
    13441 atggagatga tgatatgaat atatcatatg tgaatagcaa caaaacgaca tctttacctg
    13501 ttgagctaga tgcactaaat aataaagata tctcttatgc caaagatttt
//
LOCUS       DAZFXT010000122        13418 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.122, whole genome shotgun sequence.
ACCESSION   DAZFXT010000122 DAZFXT010000000
VERSION     DAZFXT010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13418
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.122"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(511..1602)
                     /gene="ychF"
                     /locus_tag="ACHKW0_003859"
     CDS             complement(511..1602)
                     /gene="ychF"
                     /locus_tag="ACHKW0_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HFO7476614.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1719..2303)
                     /gene="pth"
                     /locus_tag="ACHKW0_003860"
     CDS             complement(1719..2303)
                     /gene="pth"
                     /locus_tag="ACHKW0_003860"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HFO7476615.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2581..2859
                     /gene="ychH"
                     /locus_tag="ACHKW0_003861"
     CDS             2581..2859
                     /gene="ychH"
                     /locus_tag="ACHKW0_003861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HFO7476616.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="ACHKW0_003862"
     CDS             complement(2914..4593)
                     /gene="dauA"
                     /locus_tag="ACHKW0_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HFO7476617.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4718..5665)
                     /gene="prs"
                     /locus_tag="ACHKW0_003863"
     CDS             complement(4718..5665)
                     /gene="prs"
                     /locus_tag="ACHKW0_003863"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HFO7476618.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="ACHKW0_003864"
     CDS             complement(5816..6667)
                     /gene="ispE"
                     /locus_tag="ACHKW0_003864"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HFO7476619.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLIKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="ACHKW0_003865"
     CDS             complement(6667..7290)
                     /gene="lolB"
                     /locus_tag="ACHKW0_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HFO7476620.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7504..8760
                     /gene="hemA"
                     /locus_tag="ACHKW0_003866"
     CDS             7504..8760
                     /gene="hemA"
                     /locus_tag="ACHKW0_003866"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HFO7476621.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8802..9884
                     /gene="prfA"
                     /locus_tag="ACHKW0_003867"
     CDS             8802..9884
                     /gene="prfA"
                     /locus_tag="ACHKW0_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HFO7476622.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9884..10717
                     /gene="prmC"
                     /locus_tag="ACHKW0_003868"
     CDS             9884..10717
                     /gene="prmC"
                     /locus_tag="ACHKW0_003868"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7476623.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     THLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10714..11106
                     /gene="sirB2"
                     /locus_tag="ACHKW0_003869"
     CDS             10714..11106
                     /gene="sirB2"
                     /locus_tag="ACHKW0_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB2"
                     /protein_id="HFO7476624.1"
                     /translation="MTSFSTLLSVHLISLALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11110..11919
                     /gene="sirB1"
                     /locus_tag="ACHKW0_003870"
     CDS             11110..11919
                     /gene="sirB1"
                     /locus_tag="ACHKW0_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion regulator SirB1"
                     /protein_id="HFO7476625.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            11955..12809
                     /gene="kdsA"
                     /locus_tag="ACHKW0_003871"
     CDS             11955..12809
                     /gene="kdsA"
                     /locus_tag="ACHKW0_003871"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HFO7476626.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(12958..13065)
                     /gene="ldrA"
                     /locus_tag="ACHKW0_003872"
     CDS             complement(12958..13065)
                     /gene="ldrA"
                     /locus_tag="ACHKW0_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HFO7476627.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3340 a         3385 c         3463 g         3230 t
ORIGIN      
        1 gggaatttcg cttgatgtat ctgcataaca aaaacaatat gcgccatttt tgcattacta
       61 ataagaaaaa gcaaaaaaca tcgatattca tttttggtca atgggttttc tggcctatcg
      121 ttgttattgt gctactggtt atcattttca tcttgccagg atcgcacgta atcacgttat
      181 tcggggactt tatctgcggg atattaccct tcaggtaagt ccttttctcg ccagcatatt
      241 ggagcgtgat caatattgat cagctgtgaa gggggtgatg tgtagtggac caaaacttac
      301 gttatatcac tactaatgag atatccgtca tctgacactg atgtctttaa ttattctcgt
      361 ggttcactgt aggcaataag cacaaaagtg taggatgtta caagaatgat taggactcgg
      421 tgaaatgaaa aatccacgca attgcgtgga ttatatatac ttttgcgctc ttcatgagat
      481 ttagcgaaac ctcatgagac aacaaattaa ttagacgttg aaaaggaagt tcatcacatc
      541 gccatcttta acgatgtaat ctttaccttc tgcacgcatt ttgcctgctt ctttcgcgcc
      601 ttgttcacct ttgtaagtga tgaaatcttc aaacgagatg gtttgtgcac ggataaagcc
      661 tttttcaaaa tcagtatgga ttttgcccgc tgcctgcggc gcggttgctc caaccggaat
      721 ggtccatgca cgcacttctt tcaccccagc ggtgaagtaa gtttgcaggt tcagcagctt
      781 ataaccggca cggatcacac ggttcaggcc cggctcttcc agcccaagct cctgcataaa
      841 ctcgtcacgt tcttcgtcgt ccagttcggc aatgtctgct tcaacagcag cacaaaccgg
      901 aaccacaaca gaaccttctt tcgccgcgat ttcacgcacc tggtcaagat atgggttgtt
      961 ttcaaaaccg tcttcgttga cgttggcgat gtacattgtt ggtttcagcg tcaggaagct
     1021 caggtaacga atagccgctt tctcttcagc gcttaaatcc agcgcgcgca gcatacctgc
     1081 attttccaac tggggcaggc atttttccag gaccgccagc tcagctttcg cgtctttatc
     1141 gccacctttg gctttcttct gtacgcgatg aatcgcacgt tcgcaggtgt cgaggtctgc
     1201 cagcgccagt tcggtgttga taacttcaat atcgtcagcc gggttaactt tgccggaaac
     1261 gtgaatgatg ttgtcatttt caaagcagcg aacaacgtga ccgatcgctt cggtttcacg
     1321 gatgttggtc aggaactggt tacccagacc ttcgcctttc gatgcgcctt ttaccagacc
     1381 ggcgatatcg acaaattcca tggtcgtggg aagcgtacgt tgcggtttta cgatttcagc
     1441 cagttgatcc aggcgagggt caggcattgg tacgacgcct gtgttcggct caatggtgca
     1501 gaatggaaag ttggccgctt caataccggc tttggtcagc gcgttgaaca gggtagattt
     1561 cccgacgttg ggcaaaccga cgataccgca tttgaatccc atgatttaaa tcaccttaat
     1621 atcttaataa tcaacctgtt attgctaaca gattgcagaa atggaaataa ctttgcctat
     1681 tatacacggc actcggcaaa aatgccgcag acaacgactt attgcgcttt aaaggcgtgc
     1741 aatcggttcg ttgctttggt caagccatct gtaaaccaca tttcagtaca acgcgccgct
     1801 tcgtcgatgg cttcatcaat taacttctgt tcactaacag gcggtttgcc taacacaaaa
     1861 ccgacaactt tatttttatc gcccggatga ccgattccga tgcgtaaacg gtgaaagtta
     1921 gggttattac ccaatttact gatgatgtct ttcagtccat tgtgaccacc atggccaccg
     1981 cccaatttaa atttggcgac gccaggaggc agatccagtt cgtcgtgggc caccagaatt
     2041 tcgtccggat taatgcggaa aaaactggcc atcgccgcaa cggctttgcc gctgagattc
     2101 ataaatgtag tcgggactaa caggcggaca tcttcgcctc caagagtgac tcgcgaagta
     2161 taaccaaaga atttagcctc ttcgcgcagc ggagcgcgca aacgctctgc cagtaagtca
     2221 acgaaccagg caccagcatt atgtcgcgtt gcggcgtatt cagcaccggg gttcgccagg
     2281 ccgacaatca atttaatcgt cacgtttttt tgtcctgagt gtgtacataa ctggcgcgta
     2341 gtttactggt tgcggccccg cttgacaaaa aactgcgtat caaatgcaga taacgtaata
     2401 attgcatgag tggactatta gaaagtcaag gtgttcaggc gtttatttgt aaagttttgt
     2461 tgaaataagg gttgtaattg tgatcacgcc cgcacataac ccacagggtg ttgtctatac
     2521 tttacacata aggaagaggg gtattccctg ttacaaccca gaaagttccg gaggtgacat
     2581 atgaaacgca aaaacgcttc gttactcggt aacgtgctca tggggttggg tctggtggta
     2641 atggtggtcg gcgtggggta ttcaatcctc aaccagttac cacagtttaa tatgccccag
     2701 tatttcgcac atggtgcagt gctaagtatt ttcgtcggtg ccattctctg gctggcgggt
     2761 gcccgtgttg gcgggcatga acaggtgtgc gaccgttact ggtgggttcg ccactatgac
     2821 aaacgttgcc gccgtagcga taatcgccgt catagctaac aaataatgca gtttgctgac
     2881 cagtcagttt gcgctgactg gtcaatctcg tacttataaa tccgccatcg ccgcgcgacg
     2941 attcgggaag aacgccaggc gtcccgggat cggttgaatg ccagcgcgag ccatagtgcg
     3001 cagtggctgg aattccacgt tgcacacgcg cagttcacat ccctcgggca gacgcttcac
     3061 aaaacgctgg aacgcatcaa gaccaccagc atcaagtacc ggaaccgcat cccacttcag
     3121 aatcacaatc cgtttgcctt caagacgtga ctccaggtcc gtgaacaagc cttcagcagc
     3181 agcaaaaaac agcgggccaa taacgcgcag aaccaggaca tcgtctggaa catctacgac
     3241 taccggtgcc aggcgagtca tacgtgcgat acgacgcata aacagcagcg atgccagcac
     3301 gatccccacg ctgatggcaa taaccatatc aaacagcacg gtcagcgaca tgcacagcag
     3361 catgacaatg atgtcatctt tcggcgcatg acgcagcaag tcgaccactt tgtgcgcttc
     3421 actcatgttc cacgccacca tcaacagcag ggcagccatg gcggaaagcg gcagccagga
     3481 gagcagcggt gccagtacca gcagggcaag aataaccaga atagagtgga tcaccgccga
     3541 gataggggaa gttgccccgg cacggacgtt agcggcagaa cgcgcgatgg cagctgtagc
     3601 ggtaatgcca ccaaagaacg gagcgatgat attccccagt ccctgtccaa ccagttcgct
     3661 attcgccttg tgtttcgtcc cggtcatacc atccagcacc acggcgcaga gcagagattc
     3721 gattgcgccg agcattgcca ttgagaatgc cgcaggcagc agtgtgcgaa tagaatccca
     3781 ggttagcgtg aattctgaat taggcagatc ccacggcagc accagttgcg gcagcagttg
     3841 cggaataccg ttaccctgag aaccatcggc caggacgtag tggaattgcg aaccgatggt
     3901 agcaacatgt ccgccgagca ggttaacaat ccccatcacc gcgcaaccag ccagcaaggc
     3961 cggaaggtga ccgggtaaac gaatgcccag acgcggccaa aaaacaagaa tacctagcgt
     4021 cacaatgcca atggcagcat cacccacatt aatggtcggc agcgccataa ataatgcgcc
     4081 gactttttgt agataatgtt ccgggacatg ggccatttgc agaccgagaa aatctttaat
     4141 ctgcatggta ccgatggtga tcccgatacc cgaggtgaaa cctaaggtga cggaaaccgg
     4201 aatatactca atcaggcgac caaagcgtgc cagacccatc agaatcaaaa aaatccccga
     4261 cagcaaggtc gcaaccagca gtcctgccag tccaaactgt tgcgacacgg gatagagaat
     4321 taccacaaat gccgcagtcg gaccggaaac gctaaagcgt gacccacccg tcagagcaat
     4381 gacaatcccc gcaacagctg cggtatataa accgtactgg ggtgccacac cactaccaat
     4441 agccaacgcc atcgccagcg ggatagcaat aatcccgacg gttatcccgg caatcaggtc
     4501 acgggtaaac cgtgcggcag tatatttttc tttccagcaa gcgtcgatca gagcgcggaa
     4561 aggcatcaca tgtgaggaaa atattttgtt cacaataatg tttcatccgt gagcgcatca
     4621 tctgtcaact aaatggcagg tgaaggaggc ataggtcata caaatggata ttacagacaa
     4681 aaaaacccgc cgcagcgggt ctttgagccg ggttcgatta gtgttcgaac atggcagaga
     4741 tcgattcttc gttgctgata cgacgaatcg cttcggccag catacctgac agggtcagag
     4801 tacgcacgtt cggcagtgat ttgatttcat cgctcagcgg aatggtatcg cagacaacga
     4861 cttcatcaat tacagagtta cgcaggttat tcgccgcgtt gccagagaag atcgggtgag
     4921 tcgcgtacgc aaatacacgt ttagcaccac gttctttcag agcttcagca gctttacaca
     4981 gcgtaccgcc agtgtcgatc atatcatcga ccagtacgca gtcacgacct gcaacgtcac
     5041 cgatgatatg catcacctgg gaaacgttcg cacgcggacg acgtttgtcg atgattgcca
     5101 tatcggtatc gttcagcagc ttagcgatag cgcgggcacg cacaacgccg ccgatgtccg
     5161 gagaaaccac aattgggtta tccagattca gctgcagcat gtcttccagc aggatcgggc
     5221 taccaaatac gttatcaacc ggaacgtcga agaaaccctg aatctgttca gcgtgcagat
     5281 ccactgtcag cacacggtca acaccgacgc tggagaggaa gtctgcaacc actttcgcag
     5341 tgattggtac acgagcggaa cggacgcgac ggtcctggcg cgcatagcca aagtagggga
     5401 taacagcggt gatacgacct gcggaagcac gacgcagggc atcaaccata acgactaatt
     5461 ccatcaggtt gtcgttagta ggggcacaag tggactggat gatgaaaata tcaccaccgc
     5521 gtacattttc gttgatttgt acactgactt cgccgtcgct aaagcgacct acagcggcgt
     5581 cgccgagtga agtgtacagg cggttggcaa tacgttgtgc tagttccggg gtggcgttac
     5641 cagcaaaaag cttcatatca ggcacgagaa gaacctcagg catgcgtcca ttggtggaaa
     5701 gaatctgccg aaaactgtgc gggccaggca tgatcctttc caggcggtgt attaaagagc
     5761 gcgatgcaac gtctggaaca aggtgacgtt gtcaccgaaa ctcagcttgc ccggcttaaa
     5821 gcatggctct gtgcaatggg gaaagattaa cgcctttcgc cacaaagcca ttgagccatt
     5881 ccggggcttg ctctagcacc tggcgggctt cagactctgt atcaaattca gcaaagacac
     5941 aggcccctgt cccagtcagg cgcgacgggg cgtattctaa cagccaggaa agcaccgcat
     6001 caacctcgcg aaaacgtttt cttgcgataa cctcgcaatc attgctgaat tcacatttta
     6061 gcaacgtttc tattgacctt tttggcgtat tgcgcgggag ttcaggatct ttaaaaatca
     6121 ccggagtcgg aatacttaca ccagggtgcg ccaccagata ccacttctct ggcggatcca
     6181 ccggcgttag tatttcacca acgccttcgg caaacgcggc atgcccccga acaaagacag
     6241 gaacatctgc gcccagcgtc agccccattt ccgccagctc atccatgctt agcccgcatt
     6301 gccagagatg atttaatgcc accaggaccg tcgcggcatt ggatgaacca ccgccgagac
     6361 cgccgcccat cggcaaacgc ttgtcaatgc tgatattcgc accgcttccc gtcggaagac
     6421 gcccgctgtc tgccgcagtt tttatcaaca atcgcgctgc gcgaacgatc aggttatctt
     6481 catgttccac gccttcaacg ggcgttaaca gacgaatatc cccatcgtca cgaagctcaa
     6541 tgctgatggt gtcgccgtaa tcaagaaact gaaacagcgt ttgcagcgtg tggtaaccat
     6601 ccgcacgctg accggtaatg tataaaaaca gattaagttt tgccggagag ggccactgtg
     6661 tccgcattat ttcactatcc agttatccat ttttaacttg atgcgttgac caccgtcggt
     6721 gagttccata ttggctggca tcgcaggttg cgttttggtg tcataaccac cataaacaac
     6781 cttccagttt ttgccattct ggctgtaggt aatttcgctc aggcggtact ggtcgtccag
     6841 tttgtagtcg gttgcatcac ccggtaaacc taaaatccac tggcgcaagc tgttgagcgg
     6901 aattggcatt ccggtcaatt tgccaatcat ctcttcggcg tcatcggcgg tataacgctg
     6961 acctttattg tcgactaact gcacgttacc cggttgagca ttcagctcca gttccgtgct
     7021 gcccaatggg ttagtgagca gcagacggta gcgatcctgg ccggtttgct gccagaaaaa
     7081 gcgggcgtac actttttgtt ggtcagaaat ataagcgaac gcgccgcgag tctgatactg
     7141 attaagattg cgcacgtctt gctgatgctg acgccattgt ggcgaatccg ggcttttgcc
     7201 aggacctttg ggcgtggtaa cggaacaggc agtgagcaca agagcagcca gcggtagcag
     7261 gcggataaga cgaaaatcgg gcaggggcat agtgatgaca agtccttgag atacgttgca
     7321 gttataaccc ttaatgctag cgttaccgtc cgctatcgtc tatgttcaag ttgtcttaat
     7381 tgccagaatc taacggcttt cggcaattac tccaaaaggg ggcgctctct tttattgatc
     7441 ttacgcatcc tgtatgatgc aagcagacta accctatcaa cgttggtatt atttcccgca
     7501 gacatgaccc ttttagcact cggtatcaac cataaaacgg cacctgtatc gctgcgagaa
     7561 cgtgtatcgt tttcgccgga taagctcgat caggcgcttg acagcctgct tgcgcagccg
     7621 atggtgcagg gcggcgtggt gctgtcgacg tgcaaccgca cggaacttta tcttagcgtt
     7681 gaagagcagg ataacctgca agaggcgtta atccgctggc tttgcgatta tcacaatctt
     7741 aatgaagaag atctgcgtaa aagcctctac tggcatcagg ataacgacgc ggttagccat
     7801 ttaatgcgtg ttgccagcgg cctggattca ctggttcttg gggagccgca gatcctcggt
     7861 caggttaaaa aagcgtttgc cgattcgcaa aaaggtcata tgaaggccag cgaactggaa
     7921 cgcatgttcc agaaatcttt ctctgtcgcg aaacgcgttc gcactgaaac agatatcggt
     7981 gccagtgctg tgtctgtcgc ttttgcggct tgtacgctgg cgcggcagat ctttgaatcg
     8041 ctctctacgg tcacagtgtt gctggtaggc gcgggcgaaa ccatcgagct ggtagcgcgt
     8101 catctgcgcg aacataaagt acagaagatg attatcgcca accgcactcg cgaacgtgcc
     8161 caaattctgg cagatgaagt tggcgcggaa gtgattgccc tgagtgagat cgacgaacgt
     8221 ctgcgcgaag ccgatatcat catcagttcc accgccagcc cgttaccgat tatcgggaaa
     8281 ggcatggtgg agcgtgcatt aaaaagccgt cgcaaccaac caatgctgtt ggtggatatt
     8341 gccgttccgc gcgatgttga gccggaagtt ggcaaactgg cgaatgctta tctttatagt
     8401 gtggacgatc tgcaaagcat catttcgcac aacctggcgc agcgtaaagc cgcagcggtt
     8461 gaggcggaaa ctattgtcgc tcaggaaacc agcgaattta tggcgtggct gcgagcacaa
     8521 agcgccagcg aaaccattcg cgagtatcgc agccaggcag agcacgttcg cgatgagtta
     8581 accgccaaag cgttagcggc ccttgagcag ggcggcgacg cgcaagccat tatgcaggat
     8641 ctggcatgga aactgactaa ccgcttgatc catgcgccaa cgaaatcact tcaacaggcc
     8701 gcccgtgacg gggataacga acgcctgaat attctgcgcg acagcctcgg gctggagtag
     8761 cagtacatca ttttcttttt ttacagggtg catttacgcc tatgaagcct tctatcgttg
     8821 ccaaactgga agccctgcat gaacgccatg aagaagttca ggcgttgctg ggtgacgcgc
     8881 aaactatcgc cgaccaggaa cgttttcgcg cattatcacg cgaatatgcg cagttaagtg
     8941 atgtttcgcg ctgttttacc gactggcaac aggttcagga agatatcgaa accgcacaga
     9001 tgatgctcga tgatcctgaa atgcgtgaga tggcgcagga tgaactgcgc gaagctaaag
     9061 aaaaaagcga gcaactggaa cagcaattac aggttctgtt actgccaaaa gatcctgatg
     9121 acgaacgtaa cgccttcctc gaagtccgag ccggaaccgg cggcgacgaa gcggcgctgt
     9181 tcgcaggcga tctgttccgt atgtacagcc gttacgccga agcccgccgc tggcgggtag
     9241 aaatcatgag cgccagcgag ggtgaacatg gtggttataa agagatcatc gccaaaatta
     9301 gcggtgatgg tgtgtatggt cgtctgaaat ttgaatctgg cggtcatcgc gtgcagcgtg
     9361 ttcctgctac ggaatcgcag ggtcgtattc atacttctgc ttgtaccgtt gcggtaatgc
     9421 cagaactgcc tgacgcagaa ctgccggaca tcaacccagc agatttacgc attgatactt
     9481 tccgctcgtc aggggcgggt ggtcagcacg ttaacaccac cgattcggca attcgtatta
     9541 ctcacttgcc gaccgggatt gttgttgaat gtcaggatga acgttcacaa cataaaaaca
     9601 aagcgaaggc gctttcagtg ctcggtgctc gcatccacgc tgctgaaatg gcaaaacgcc
     9661 aacaggccga agcgtctacc cgtcgtaacc tgctggggag tggcgatcgc agcgaccgta
     9721 accgtactta caatttcccg caggggcgcg ttaccgatca ccgcatcaac ctgacgctct
     9781 accgcctgga tgaagtgatg gaaggtaagc tggatatgct gattgaaccg attatccagg
     9841 aacatcaggc cgaccaactg gcggcgttgt ccgagcagga ataatggaat atcaacactg
     9901 gttacgtgaa gcaataagcc aacttcaggc gagcgaaagc ccgcggcgtg atgctgaaat
     9961 cctgctggag catgttaccg gcagagggcg tacttttatt ctcgcctttg gtgaaacgca
    10021 tctgactgac gaacaatgtc agcaacttga tgcgctactg acacgtcgtc gcgatggtga
    10081 acccattgct catttaaccg gggtgcgaga attctggtcg ttgccgttat ttgtttcgcc
    10141 agcgacctta attccgcgcc cggatacgga gtgtctggtg gagcaggcac tggcgcggtt
    10201 gcctgaacaa ccttgccgta ttcttgatct cgggacgggt accggggcga ttgcgcttgc
    10261 gctggctagc gagcgcccgg actgcgaaat tatcgctgta gatcgtatgc ctgatgctgt
    10321 ctccctggca caacgtaatg cccagcatct ggcgatcaaa aatatccaca ttctgcaaag
    10381 cgactggttt agcgcgctag ccgggcagca gtttgcgatg attgtcagca atccgccgta
    10441 tattgacgag caggacccac atcttcaaca aggcgatgtc cgctttgagc cgctcactgc
    10501 gctggttgcg gcagacagtg gaatggcaga catcgtgcat atcatcgaac agtcgcgtaa
    10561 cgcgctggta tccggcggct ttctgcttct ggaacatggc tggcagcagg gcgaagcggt
    10621 gcgacaagca tttatcctcg cggggtatca tgacgtcgaa acctgccgtg actatggtga
    10681 taacgagcgc gtaacgctcg gccgctatta tcaatgacaa gtttttctac actgcttagt
    10741 gttcatctta ttagtctcgc gctttctgtt gggctattaa ccttacgttt ctggctacgt
    10801 tatcagaagc atccacaggc atttgctcgc tggacgcgca ttgtgccgcc ggttgtcgat
    10861 acggtgttat tgttaagcgg cattgcgttg atggctaaag cgcacatcct gccattttcc
    10921 gggcaggcac agtggctgac tgaaaagctg tttggagtta tcatttatat cgttttgggt
    10981 tttattgcac tcgattatcg tcgtatgcac agtcagcagg cgcgcattat tgccttcccg
    11041 ctggcgttgg tggtgctgta catcatcatt aaactcgcca ccacaaaagt accgttactg
    11101 gggtaagtca tgagatcgtt agctgatttc gaatttaata aagcgccatt gtgcgaaggc
    11161 atgatcctgg cttgcgaagc aatccgccgc gattttccct cgcaagatgt ttacgacgaa
    11221 ctggagcgtc tcgttagtct ggcgaaggaa gaaatcagcc agcttctgcc tttagaagag
    11281 cagctggaaa aactgatcgc gctgttttac ggcgactggg gatttaaagc ctcacgcggc
    11341 gtttatcgtc tttccgatgc attatggctg gaccaggtgt taaagaatcg acagggcagt
    11401 gcggtatcat taggtgcggt tttattatgg gtcgcgaatc gtctcgattt gccgctgctg
    11461 ccggtgattt tccctacgca gctgatattg cgcattgaat gtccggatgg cgaaatttgg
    11521 ctgattaatc cttttaacgg tgaatcgtta agcgaacata tgctggacgt atggttaaag
    11581 ggaaatatca gcccgtcggc ggaactgttt tatgaagacc ttgatgaagc tgataacatt
    11641 gaggtaatcc gcaaattgct ggatacactc aaagcctcgt tgatggaaga aaatcagatg
    11701 gagctggcgt tacgcaccag cgaagcttta ttacaattca accctgaaga tccctatgaa
    11761 attcgcgatc gcgggttgat ttatgcgcaa ctggattgcg aacacgttgc gttgaacgat
    11821 ttaagttatt tcgttgaaca gtgtccggaa gacccgatca gcgaaatgat ccgtgcgcaa
    11881 ataaataaca tcgcgcataa acatattgtg ctgcattaat taatcgacat tttactcaag
    11941 attaaggcga tcctatgaaa caaaaagtgg ttagcattgg cgacatcaac gtagcaaatg
    12001 acctgccgtt cgtactgttt ggcggtatga acgtgctgga atctcgcgat ctggcgatgc
    12061 gcatttgcga gcactacgta actgtgaccc agaaactggg tatcccttac gtgttcaaag
    12121 cctcttttga caaagccaac cgctcctcca tccactctta tcgtggaccg ggcctggaag
    12181 aagggatgaa aatcttccag gagttgaagc agacttttgg cgtgaaaatt atcaccgacg
    12241 ttcacgaacc aagtcaggca cagcccgttg ctgatgtcgt ggatgtgatt cagttgccgg
    12301 cgtttcttgc tcgccagact gacctggttg aagccatggc gaaaaccggt gcggtaatta
    12361 acgtcaagaa accacagttt gtcagcccgg gacagatggg taatatcgtt gataaattca
    12421 aagaaggcgg caacgaaaaa gtgattcttt gcgatcgtgg tgctaacttc ggctatgaca
    12481 acctggttgt cgatatgctg ggcttcagca ttatgaagaa agtgtctggt aactcgccgg
    12541 tgattttcga cgtgacccac gcactgcaat gccgcgatcc gtttggcgca gcttccggtg
    12601 gtcgtcgtgc tcaggtggct gagctggcac gagccggtat ggcggtaggt ctggcggggc
    12661 tgtttattga agcgcatccg gatccggaac atgcgaaatg tgatggtcca tccgcgctgc
    12721 cgctggctaa actggaaccg ttcctcaagc agatgaaagc gattgatgat ctggtgaaag
    12781 gtttcgaaga actggatacc agcaagtaat cttttttgct tgaaaaataa agtcttagcg
    12841 ttctgcgtta agactttttt catgggtgcc agatacaaaa aaggccgcag gctgttaccc
    12901 ctgcggccgg tttcgggcgc atattgccat cacggcagcc tgacgcccgt tttcacctta
    12961 cttccggtta cgccaccagc cgacaatcgc tgcggtaata attcccgcca ggatcggtgc
    13021 tgccaggtcg tgccagaaaa tcatggcaaa ctgcgcgagc gtcatatagc cgccttgttg
    13081 taatgacaac attttgcggc tattcttgaa ttgttctggt tcaagattag cccccgttct
    13141 gttgtcaggt taaacctctc aacgtgcggg ggttttctct ttccagcaac caatgccacc
    13201 agggataaag cccccgcaac attgcgcctc accggataac gccggcttga tgtggatact
    13261 acgtctcaat tcatcttcac ttcatccctg aaatgtttgc aatgaagagt gcattccggt
    13321 ttttcaacag ctgttacagt cgtttcatga gggctctgga tgatgcttcc agctcgggtt
    13381 gccaatattt acttgtggaa gagataaaga caaaaatg
//
LOCUS       DAZFXT010000123        13174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.123, whole genome shotgun sequence.
ACCESSION   DAZFXT010000123 DAZFXT010000000
VERSION     DAZFXT010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.123"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..262
                     /gene="yddL"
                     /locus_tag="ACHKW0_003873"
                     /pseudo
     CDS             1..262
                     /gene="yddL"
                     /locus_tag="ACHKW0_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
     gene            301..1632
                     /locus_tag="ACHKW0_003874"
     CDS             301..1632
                     /locus_tag="ACHKW0_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HFO7476628.1"
                     /translation="MITDLILHNNPRMKTITLNDNHIAHLNAKNTTELEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNTLESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNDLFPNLNTLLINNNLLSEIKIFYSNFCNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNNCPTMQ
                     LMLFNLFSPALKLNTGLAILSPGAFEVHSDGIDVDNELFHYPIKKAYTPYNIHTYKTE
                     EVVNQRNIKVKNMTLDEINNTYCNNDYYNQAIREEPIDLLDRSFSSSSWPF"
     gene            1956..3344
                     /gene="narU"
                     /locus_tag="ACHKW0_003875"
     CDS             1956..3344
                     /gene="narU"
                     /locus_tag="ACHKW0_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HFO7476629.1"
                     /translation="MALQNEKNNRYLLRDWKPENPTFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            3426..7166
                     /gene="narZ"
                     /locus_tag="ACHKW0_003876"
     CDS             3426..7166
                     /gene="narZ"
                     /locus_tag="ACHKW0_003876"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HFO7476630.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSASQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGSKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            7163..8707
                     /gene="narH"
                     /locus_tag="ACHKW0_003877"
     CDS             7163..8707
                     /gene="narH"
                     /locus_tag="ACHKW0_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0042126 - nitrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HFO7476631.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAIQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            8707..9402
                     /gene="narW"
                     /locus_tag="ACHKW0_003878"
     CDS             8707..9402
                     /gene="narW"
                     /locus_tag="ACHKW0_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051131 - chaperone-mediated protein
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HFO7476632.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRSDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     SLSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            9399..10079
                     /gene="narI"
                     /locus_tag="ACHKW0_003879"
     CDS             9399..10079
                     /gene="narI"
                     /locus_tag="ACHKW0_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009325 - nitrate reductase complex
                     [Evidence IEA];
                     GO_function: GO:0008940 - nitrate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HFO7476633.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            10158..11051
                     /gene="yddE"
                     /locus_tag="ACHKW0_003880"
     CDS             10158..11051
                     /gene="yddE"
                     /locus_tag="ACHKW0_003880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HFO7476634.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEL"
     gene            complement(11147..11992)
                     /gene="nhoA"
                     /locus_tag="ACHKW0_003881"
     CDS             complement(11147..11992)
                     /gene="nhoA"
                     /locus_tag="ACHKW0_003881"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HFO7476635.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDGWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            12165..12734
                     /gene="yddH"
                     /locus_tag="ACHKW0_003882"
     CDS             12165..12734
                     /gene="yddH"
                     /locus_tag="ACHKW0_003882"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HFO7476636.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDNKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(12738..12965)
                     /gene="pptA"
                     /locus_tag="ACHKW0_003883"
     CDS             complement(12738..12965)
                     /gene="pptA"
                     /locus_tag="ACHKW0_003883"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HFO7476637.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(13064..>13174)
                     /locus_tag="ACHKW0_003884"
     CDS             complement(13064..>13174)
                     /locus_tag="ACHKW0_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HFO7476638.1"
                     /translation="ILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGRRLS"
BASE COUNT         3305 a         3319 c         3287 g         3263 t
ORIGIN      
        1 agttgcggtg gttgtaactg gtttgttagc tgcgaacgta gcacacgctg ccgaagtcta
       61 taacaaggat ggtaataaac tcgaccttta tggcaaggtt accgctctac gttattttac
      121 tgatgataag cgtgacgatg gtgataaaac ttatgcccgt ctcggcttta aaggagaaac
      181 gcaaatcaat gatcaaatga ttggttttgg tcactgggaa tatgatttta aaggctataa
      241 cgatgaagcc aacggttcgc gcggcaacaa tctctgattg agttaagtgc ccatcacaat
      301 atgatcactg accttatatt acacaataat cccaggatga aaacaatcac tttaaacgac
      361 aaccatattg cacatttaaa cgccaaaaac actacagaac tggaatattt aaacttaagc
      421 aataacaatt tactgccaac caatgacatt gatcaactaa tatcatcaaa acatctttgg
      481 catgtattag ttaacggcat caacaatgat ccacttgcac aaatgcagta ctggactgca
      541 gtaagaaata taattgatga cactaatgaa gtgaccattg atttatcagg acttaattta
      601 accactcaac caccagggct gcaaaacttc acctctatca atcttgataa taaccaactc
      661 acacattttg atgcaaccaa ctacgataga ctcgtaaagc taagtctgaa tagtaatact
      721 cttgagtcaa taaattttcc tcaaggcaga aatgtaagta ttacacatat atctatgaat
      781 aataatgctc tcagaaatat tgatatagat aggctttcat cagttactta ttttagtgcg
      841 gcacataatc aactagagtt tgtgcaatta gaatcttgcg aatggctgca atacctgaat
      901 ctcagccata atcaattaac tgatattgtt gcaggtaata aaaatgaact cttactgctg
      961 gatctatccc ataataaact aacaagttta cacaatgact tatttcccaa cttgaatacg
     1021 ttacttatta acaacaattt gctttctgaa attaaaatat tctatagcaa cttctgcaat
     1081 gttcagacat taaacgctgc taacaaccag ttgaaatata taaatcttga tttcctgact
     1141 tatcttccat ctatcaaaag tttaagactg gacaataata aaataaccca cattgatact
     1201 aataatacat ccgatattgg aactttattc cccataataa aacagagcaa aaacttaaat
     1261 tttttaaatg tttctgggaa gaacaattgc cctactatgc agctcatgtt atttaatttg
     1321 ttttccccag cacttaagct taatactggc ctggcaattc tttcgcctgg tgcatttgaa
     1381 gttcactctg acggaataga tgtggataac gaattgtttc actatcctat taaaaaagca
     1441 tataccccat ataatataca cacttacaag acagaggaag ttgtaaacca gaggaatata
     1501 aaagttaaaa acatgacctt agatgaaata aacaatactt actgtaataa cgattattac
     1561 aatcaggcaa taagagagga accgatagac cttctggaca gatcgttttc ctccagttca
     1621 tggccttttt agtgaataaa tcatcactaa ttctataagt gtctggtctt gttgataata
     1681 tagttaatat gtaacccttt agattcacga tctgaagggt tgaaacatga catacgttca
     1741 aagattaacc cccgtttgaa ggttagcttg ttagttacat ttagtaacac atatctggct
     1801 acttaatccc cattctgccc ccgcggttac gctttcttat ctattctttc aatcagcact
     1861 tcacccaatt catccttaat tctaataact ggctattgcg ccttacctcc tgttttgtct
     1921 tttttcatta ccaatgtgtg catgtgagga acaatatggc actgcaaaat gagaaaaata
     1981 atcgttatct tttacgcgac tggaaaccag aaaatccgac cttctgggaa aataaaggaa
     2041 aacatattgc tcgaagaaac ctctggatat cagtcagttg tctacttctt gccttctgtg
     2101 tctggatgct atttagcgca gttaccgtta atctcaataa aatcggtttt aattttacta
     2161 ccgatcaact ctttttatta accgcattac cctccgtttc tggcgcatta ttgcgtgttc
     2221 cctactcctt tatggtgcct atattcggtg gacgccgatg gacggttttt agtactgcaa
     2281 tcctgattat tccttgcgtc tggctcggaa ttgccgtgca aaatccgaat actccttttg
     2341 ggatatttat cgttatcgct ttgctatgcg gttttgcagg tgcaaacttt gcttcgagca
     2401 tgggcaatat cagtttcttc tttccaaaag ccaaacaagg gagcgctctt gggattaatg
     2461 gcggattagg aaacttaggt gtaagtgtaa tgcagctggt tgcaccgctg gtcatttttg
     2521 tacctgtatt tgcctttctc ggcgtcaatg gcgtaccgca ggccgacggt tcggtgatgt
     2581 cgctggcgaa tgccgcatgg atttgggtac cgctactggc gattgccacg atcgccgcct
     2641 ggtcagggat gaatgatatc gccagttcac gcgcctcaat tgccgaccag ctccctgtct
     2701 tacaacgcct gcatctctgg ctgctgagcc tgctttacct tgccaccttc ggttcgttta
     2761 tcggtttttc tgcgggtttt gccatgctgg caaaaaccca gttcccggat gtgaatattc
     2821 tgcgcctggc gttctttggc ccatttatcg gtgccatcgc gcggtcggtt ggtggtgcta
     2881 tttccgataa gttcggcggc gtgcgggtga cgttgatcaa ctttattttt atggcgattt
     2941 tcagtgccct gctgttcctt accttaccgg gcacaggctc cggtaatttc atcgcctttt
     3001 acgccgtatt tatggggctg tttctgactg cgggtctggg aagtggttct actttccaga
     3061 tgatcgccgt catctttcgc cagataacca tttatcgggt aaagatgaaa ggcggtagtg
     3121 atgagcaagc tcagaaagaa gccgtcaccg aaacggcggc ggctctgggc tttatctcag
     3181 ccattggcgc agtgggcggc ttttttattc cgcaggcgtt tggcatgtcg ctcaatatga
     3241 ccggctctcc ggtcggcgcg atgaaagtgt ttttaatctt ctacatcgtt tgtgtgctgc
     3301 tgacctggct ggtttatggt cggcggaagt tcagccaaaa ataagagatc gatgcctgat
     3361 gtacaaccgt gtcgcatccg acaaggcatc agatcccaat tcattcctgg agcaggagtc
     3421 atgtcatgag taaacttttg gatcgctttc gctacttcaa acaaaagggc gaaacctttg
     3481 ccgatggtca cggacaggtg atgcatagca accgcgactg ggaggacagc tatcgccagc
     3541 gttggcagtt cgacaaaatc gtgcgttcca cccacggtgt taactgtaca ggctcctgta
     3601 gctggaaaat ctacgttaaa aatggtctgg tgacctggga aatccaacag accgactacc
     3661 cgcgcactcg ccctgacctg cccaatcatg aacctcgcgg ctgcccgcgt ggcgcaagtt
     3721 actcctggta tctttacagc gctaaccgcc tgaaataccc gctcattcgt aaacgactga
     3781 ttgaactgtg gcgcgaagcc ctcaaacaac acagcgatcc ggtactggcg tgggcatcga
     3841 ttatgaacga tccgcaaaag tgcctgagct acaaacaagt gcgtgggcgc ggcgggttta
     3901 tccgctccaa ctggcaggaa ctaaaccagc tgattgccgc cgctaacgtc tggaccatca
     3961 aaacctacgg cccggatcgc gttgccggtt tctcgccgat cccggcgatg tcgatggttt
     4021 cttacgccgc cggaacgcgt tatctgtcgc tgcttggcgg cacctgttta agtttctacg
     4081 actggtattg cgacctgccg cccgcctcgc cgatgacctg gggcgagcaa accgacgtac
     4141 cggaatctgc cgactggtat aactccagct acatcatcgc ctgggggtct aacgtaccgc
     4201 agacacgtac gccggacgcc cacttcttta ccgaagtacg ctacaaaggc actaaaacca
     4261 tcgccattac ccctgactac tctgaagtgg ccaaattgtg cgaccagtgg ctggcaccga
     4321 aacaaggcac tgatagcgcc ctggcgatgg caatgggcca tgtgatttta aaagagtttc
     4381 atctcgataa tcccagcgac tactttatca actactgccg ccgctacagc gacatgccga
     4441 tgctggtaat gctggagcct cgcgacgatg gtagctacgt tcccgggcgc atgatccgcg
     4501 catctgacct ggtggatgga ctgggcgaaa gcaacaatcc gcagtggaaa accgtagcag
     4561 ttaataccgc aggtgaattg gtagtgccga acggttcgat tggtttccgc tggggagaaa
     4621 aaggcaaatg gaatctggaa tccattgccg ccggtacgga aaccgaattg tcgttaaccc
     4681 tgctcggtca acatgacgct gttgcaggcg tggccttccc ctactttggc ggcattgaaa
     4741 atccgcattt tcgcagcgta aaacacaatc cggtgctggt gcgccaattg cccgttaaaa
     4801 acctgacgtt agtcgatggc aacacctgtc cggtggtcag cgtttatgat ttggtactgg
     4861 cgaattacgg cctcgatcgc gggctggaag atgaaaacag tgcgaaagat tacgctgaaa
     4921 tcaaaccgta caccccagcc tggggtgagc aaattaccgg cgtgccgcgc cagtatattg
     4981 aaaccatcgc ccgtgaattt gccgataccg cccataaaac gcatgggcgc tcgatgatta
     5041 tcctcggcgc aggtgttaac cactggtatc acatggatat gaactaccgt gggatgatca
     5101 atatgctgat cttctgcggt tgtgtcgggc aaagcggtgg cggctgggca cactatgtcg
     5161 gccaggaaaa actgcgccca caaaccggct ggttgccact ggcctttgcg ctcgactgga
     5221 accgaccacc gcgccaaatg aacagcacct cgtttttcta caatcattcc agccaatggc
     5281 gctatgaaaa agtctctgct caggagttac tttcaccgct cgccgatgcc agtaagtaca
     5341 gcggtcatct gattgatttc aacgttcgcg ccgaacgtat gggctggcta ccttctgcgt
     5401 cgcagctggg gcgtaacccg ctcgggatta aagctgaagc cgacaaggcc ggattatccc
     5461 ccacagaatt taccgcccag gcgctgaaat cgggcgattt acgtatggcc tgcgaacaac
     5521 cagatagcgg cagcaatcat ccgcgtaatt tgtttgtctg gcgctctaac ctgcttggct
     5581 cctccggcaa aggccacgag tatatgcaga agtatctgct ggggaccgaa agcgggattc
     5641 agggcgagga actcggtgcc agcgacggga gcaaaccgga agaagtcgag tggcaaactg
     5701 cggcgattga aggcaagctc gacctgctgg tgacgctcga cttccgcatg tccagtacct
     5761 gcctgttctc cgatatcgtt ctgcccaccg ccacctggta cgaaaaagac gatatgaaca
     5821 cctcggatat gcatccgttt attcatccgc tttctgcggc ggtcgatccg gcgtgggaat
     5881 cacgcagcga ctgggaaatc tacaaaggta tcgccaaagc attttcgcaa gtgtgcgtgg
     5941 gtcatcttgg caaagaaacc gacgtggtat tacaacccct actgcatgac tctccggcag
     6001 agctctcaca gccgtgtgaa gtgctcgact ggcgcaaagg cgaatgcgat ctgattccag
     6061 gcaaaaccgc gccgaatatt gtggcggtgg agcgcgacta ccctgctacg tatgaacgct
     6121 ttacctcgct cgggccattg atggacaaac ttggcaatgg cggtaaaggg atttcgtgga
     6181 atacgcagga tgaaatcgat ttcctcggca aactcaatta caccaagcgt gatggcccgg
     6241 cacaggggcg tccgctgatt gacaccgcca ttgacgcttc agaagtgatt ctggcactgg
     6301 caccggaaac caacggtcat gttgcagtca aagcgtggca ggcgctgggc gagatcaccg
     6361 ggcgcgaaca tacccatctg gcgctgcaca aagaggacga gaagattcgc tttcgcgata
     6421 ttcaggcgca gccgcgtaaa attatctcca gccccacctg gtccggtctg gaaagcgatc
     6481 acgtatccta taacgcggga tacaccaacg ttcatgagtt aattccgtgg cgcacgctgt
     6541 cgggacgcca gcagctctat caggatcatc cgtggatgcg tgcttttggt gaaagcctgg
     6601 tggcttatcg cccgcctatc gacacccgta gcgtcagtga gatgcgccag ataccgccaa
     6661 acggcttccc ggaaaaagca cttaacttcc tgacgccgca ccagaaatgg ggcattcact
     6721 caacctacag tgaaaacctg ctaatgctga cgctctctcg cggtggaccg attgtctgga
     6781 tcagcgaaac agatgcccga gaactgacca ttgtcgataa cgactgggtg gaagtattca
     6841 acgccaacgg cgcgctgact gcccgcgcgg tggtcagcca acgtgtaccg ccgggcatga
     6901 ccatgatgta tcacgcccag gaacgcatta tgaatattcc tggttcggaa gtaactggca
     6961 tgcgcggcgg cattcataac tcggttaccc gcgtttgccc gaaaccgacg catatgattg
     7021 gcggttacgc gcagctggcc tggggcttta actactacgg caccgtcgga tcgaaccgcg
     7081 atgagttcat catgatccgc aagatgaaga acgttaactg gctggatgat gaaggtcgcg
     7141 atcaggtaca ggaggcgaaa aaatgaaaat ccgttcacaa gtcggcatgg tgcttaacct
     7201 cgacaaatgt atcggctgcc atacctgttc ggtgacctgt aaaaacgtct ggaccgggcg
     7261 cgaaggcatg gagtacgcat ggtttaacaa cgtcgaaacc aaaccgggca ttggttatcc
     7321 gaaaaactgg gaagatcagg aagagtggca aggcggctgg gtccgcgatg tgaatggcaa
     7381 gatccgtccg cgtctgggca gcaagatggg cgtaataacc aaaatcttcg ccaacccggt
     7441 ggtgccgcag attgatgatt actacgaacc tttcactttc gactacgaac atttgcatag
     7501 cgcaccggaa ggcaaacata tccctactgc tcgcccgcgc tcgctgattg acggtaagcg
     7561 gatggacaaa gtgatctggg ggccaaactg ggaagaactg ctgggcggcg agtttgaaaa
     7621 acgtgcccgc gaccgcaact tcgaggccat acaaaaggag atgtacgggc agtttgaaaa
     7681 caccttcatg atgtacctgc cgcgcctgtg cgaacactgc ctcaatccca gttgcgtggc
     7741 gacctgccca agcggcgcta tctacaaacg cgaagaagac ggcattgtgc tgattgatca
     7801 ggataaatgc cgtggctggc gtttgtgcat tagcggttgt ccgtacaaaa aaatctactt
     7861 caactggaaa agcggcaagt cagaaaaatg tatcttctgt tatccgcgaa ttgagtcagg
     7921 ccaacccacc gtgtgctcag aaacctgcgt gggtcgcatc cggtatctgg gcgtgctgct
     7981 ttacgacgcc gaccgcatcg aggaagcggc gagcaccgag cgcgaagttg acctctatga
     8041 acgtcagtgc gaagtgttcc tcgatccaca cgatccctca gtgatcgagg aagccctgaa
     8101 acaaggtatt ccacaaaacg tgattgaagc tgcccagcgt tcgcctgtct acaaaatggc
     8161 gatggactgg aaactggcgc taccgctgca ccctgaatat cgcaccctgc cgatggtctg
     8221 gtacgttcct ccgctgtcgc cgattcagtc ctacgcagat gcgggcggtt tgccgaaaag
     8281 cgaaggcgtg ctgcccgcca tcgaaagcct gcgtattccg gtgcaatatc tcgccaatat
     8341 gttgagtgcc ggcgataccg gtccggtact gcgggcgctg aaacggatga tggcgatgcg
     8401 ccactatatg cgttcacaaa ccgtggaagg cgttactgat actcgtgcca tcgacgaagt
     8461 aggcctgagc gtcgcccagg tcgaagagat gtatcgttac ctcgccattg ccaactatga
     8521 agatcgtttt gtcatcccga cgagccatcg ggaaatggcg ggcgatgcct tcgcagaacg
     8581 caacggctgc ggttttacct ttggcgacgg ttgccacggt tcggacagta aattcaacct
     8641 gttcaacagt agccgtatcg atgccatcaa catcaccgaa gtgcgcgaca aagcggaggg
     8701 cgaataatgc agatcctcaa agtgatcggc ctgttgatgg agtatccgga cgagctgttg
     8761 tgggaatgca aggaggacgc gctggcgttg atccgcagcg acgcgccgat gcttacggat
     8821 ttcacccaca acctgcttaa cgcgccgctg ctggataaac aggccgaatg gtgcgaagtg
     8881 tttgaccgtg gacgtaccac atcgctgctg ctgttcgaac atgttcatgc cgagtcccgc
     8941 gatcgcggcc aggcaatggt ggacctgctg gcggagtatg aaaaggtcgg cctgcagctg
     9001 gattgtcggg aactgcccga ttatctaccg ctgtatctgg agtatttaag cgtactgcct
     9061 gacgatcagg cgaaagaagg attgctcaat gttgcgccga tcctcgccct gcttggcggt
     9121 cgcttaaaac aacgcgaggc accgtggtac gcgttgtttg atgctctgct gcaactggcg
     9181 ggaagctccc tttcaagtga cagtgtcaca aaacaagtaa acagcgaaga gcgcgatgac
     9241 acccgccagg cgcttgatgc agtgtgggaa gaggaacagg tgaagtttat tgaagataac
     9301 gccacggcgt gtgacagctc gccgcttaat caatatcagc gacgctttag ccaggatgtc
     9361 gcgccgcaat atgtcgacat cagtgcggga ggtgggaaat gattcagtat ctgaacgtct
     9421 ttttttacga tatctacccg tacatttgtg cgacggtgtt tttcctcggc agctggctgc
     9481 gctacgacta cgggcagtac acctggcgcg cctcctcaag tcaaatgctc gataaacgcg
     9541 ggatggttat atggtcgaat ctgttccata tcggcatttt ggggatcttc ttcgggcacc
     9601 tgttcggcat gttaacgccg cactggatgt acgcgtggtt tttgccagtg gcagcgaaac
     9661 aactaatggc gatggtgctc ggcggtattt gcggtgtgtt gacattgatt ggcggcgcag
     9721 gactgctgtg gcgcaggctg acaaatcagc gcgtgcgtgc aacttccact acgccggata
     9781 ttatcatcat gagcattttg ctgatccagt gtctgctggg gctaagtacc ataccgtttt
     9841 cagcacagta tcctgatggt agcgaaatga tgaagctggt gggttgggcg caaagcattg
     9901 tcactttccg tggtggatcg tcagaaatgc ttaacggcgt agcgttcgtt ttccgcctgc
     9961 atctggtatt gggaatgacc atcttcctgc tcttcccgtt caccagattg gtgcatgtgt
    10021 ggagcgcgcc gtttgagtac tttactcgtc ggtatcagat tgtgcgttcg cggcgataat
    10081 ttcaccctgg ccggatagcc cttcgctctc cggccacttg caggctatga tagttttcca
    10141 gtcacaggag aacgttaatg aaaccgcagg tgtaccacgt cgatgctttt acctctcaac
    10201 catttcgcgg caattctgcc ggtgttgttt tccccgccga taatcttagc gaagcgcaaa
    10261 tgcagcttat cgcccgcgag ttaggccatt cggaaactgc ttttctgctg cacagcgacg
    10321 acagcgatgt gcgcatccgc tactttacgc caacggttga agtgccgatt tgcggtcacg
    10381 cgacagtagc tgcgcactat gtacgtgcga aggtgttagg tttaggaaat tgcacgatct
    10441 ggcaaacatc gctggcagga aaacatcgcg tgactatcga aaagcacaac gatgattatc
    10501 gtatttcgct ggaacaaggt acgccgggct ttgagccacc gctggaaggt gaaacacgtg
    10561 aggcgattat caacgcgctt catctcactg aagacgatat tttgccgggc ttgccaattc
    10621 aggtcgcaac aacaggacac tcaaaagtga tgatcccgtt gaaaccggaa gtggatatcg
    10681 acgccctttc gcctgatctt gctgcgctga gcgctatcag caaacagatt ggctgcaatg
    10741 gtttcttccc gttccagatc cgcccaggca aaaacgaaac cgatggtcgt atgttctcgc
    10801 ctgcgattgg tattgtggaa gatccggtga ctggcaatgc caatggcccg atgggtgcat
    10861 ggttggtaca tcacaacgta ttgccccacg atggcaacgt gttatgtgtt aaaggccatc
    10921 aggggcgcgc attggggcgt gacggcatga ttgaagtgac ggtgacaatt cgtgataacc
    10981 aaccggaaaa agtcaccatt tcaggcacag cggtgatttt gtttcatgcc gaatgggcaa
    11041 ttgaactctg aaaccgttgc ctgatgcgct tcgcctgtca ggtctacaac cgagctaatg
    11101 tatgccggat aaggcgttta cgccgcatcc ggcaacctga cataaattat tttcccgcct
    11161 ccggatgcgt atcaaacgcc gccatcacca gcgctaactc atccacagta aagccatgtt
    11221 tcgcatcatc cacgcccaga ccaaactgtt cctgcatcac ggcatataac gacaccacat
    11281 ccggtagatt tcgctgctcc accgcgtgcc cattttcata atgggtaaaa tgaaaattgg
    11341 tcagcgttag cttgccaccg tccggcaaat ggcggcacat cagcaaatga tggcgaaaat
    11401 gcgactgcgg ccagtgcgcc gaccagaaat tgcccatcac ataatcgctt tgttgctgct
    11461 cgcagagatc aaaacggtac atcgactgcc aatgctgatg atgattaaac tgcaacaccc
    11521 aaccatcacc ctcctgcaac aaccgatact ctccgtgtgg cgtggtctgc acgagatcgg
    11581 aaactaaacg aatcggcgcg gttagcgtct gcccaccgaa accgacatca gcaatccatt
    11641 tttcctcttc cagttccacc aacagcaaac gatgggtgcg cggcggtaat gctggcggat
    11701 ttgataacac tacgcgccct aacaagctgc gaacgttaaa ccccagctcg cgtaacaccc
    11761 gctcaaacac gccattctgc tcaaaacagt aaccgccacg acgggctatc accagtttct
    11821 cttccagcga ttgatcatca agctgtattt ccctcggcag caaaacgtca aggttttcaa
    11881 acggaatagt gcaattgtgt ttcaggtgca atgcacgaag cgtatcaata ttgaccgcag
    11941 cagctcccga ccagttaata cgggcaaaat agtgattcag aatgggcgtc atcaggcgtt
    12001 tcccttagtg gttttctcaa cttataacct ggaatagacg ccgcaccggc tattttgtgc
    12061 gatcgcggac atgcatcacg tttgcaaatc gccaatcccg ttaatttcgt tacttattgc
    12121 gttacggact acgcttaaaa aaaccttctt cgcgaggcga taccatgagc cgattcatcc
    12181 ccatagaatt acaccatgcc agccgtctgt taaatcatgg cccaactgtc atgatcacca
    12241 gtttcgatga gcaatcccag cggcgtaaca ttatggcagc agcctggtca atgccggtgg
    12301 agtttgaacc gccacgcgtg gcgattgtgg tagataaatc gacatggacc agggagttga
    12361 ttgaacgtaa cggtaaattt ggcatcgtta tcccgggcgt tgcagcaact aactggacgt
    12421 gggcagtggg aagcgtgtcg ggtcgtgaag aagataaatt taattgctat ggcattccgg
    12481 tggtgaaagg tccggtgctt ggtttgccac ttgttgaaga gaaatgcctg gcgtggatgg
    12541 agtgccgcct gctacctgcg acttctgcgc aacaaaaata cgacacgctg tttggcgagg
    12601 tggtatcagc agcggcagac gcacgggtat ttgtcgaagg ccgctggcag tttgatgata
    12661 ataagctcaa tacgttgcat catttaggtg ctggcacgtt tgttactagc ggcaagcgtg
    12721 ttacggcagg ttaagcatta catgctgtaa ccgggtttct ttatcaaagc ctccatttga
    12781 ggcgcgattt cggcatccca gatagcttgc caagattctg gttgaatctg ctgtagagca
    12841 atgcttatcg aactgtcttt actgttcaga tggcgaataa taacgtcggt aatatctgca
    12901 gcaagtgctg ctttttgttg ttcgtccagt tcacgcggaa agcatttaat gtcgatgtgc
    12961 ggcatgatta cgctcctttt gaaagatgtc agtaaaatta acacacattg tgaatgtcat
    13021 gtgtccggta aagcgtttat acgacaataa tgccagggca agattacgaa agccttctcc
    13081 ccgcaaggac tgacgccagg tagtttctgt ccatggctgc ttttcgcatc ttacgtctta
    13141 accctgcctt gaatacctta tcattcgtca aaat
//
LOCUS       DAZFXT010000124        13129 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.124, whole genome shotgun sequence.
ACCESSION   DAZFXT010000124 DAZFXT010000000
VERSION     DAZFXT010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.124"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..203)
                     /locus_tag="ACHKW0_003885"
     CDS             complement(<1..203)
                     /locus_tag="ACHKW0_003885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476639.1"
                     /translation="MPEQAITKALCIYQGQQINLAYIRLRHFEFTNGRIISDFNGMGK
                     VKYHFLTIKRVLFLVYQTISIHS"
     gene            140..1639
                     /gene="pitB"
                     /locus_tag="ACHKW0_003886"
     CDS             140..1639
                     /gene="pitB"
                     /locus_tag="ACHKW0_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HFO7476640.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLTV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1688..2380)
                     /gene="yghT"
                     /locus_tag="ACHKW0_003887"
     CDS             complement(1688..2380)
                     /gene="yghT"
                     /locus_tag="ACHKW0_003887"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7476641.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDVEK
                     AITDFMTARGYH"
     gene            2554..3267
                     /gene="yghS"
                     /locus_tag="ACHKW0_003888"
     CDS             2554..3267
                     /gene="yghS"
                     /locus_tag="ACHKW0_003888"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006235 - dTTP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HFO7476642.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     HPADEILQASLRAIHAALS"
     gene            3299..4056
                     /locus_tag="ACHKW0_003889"
                     /pseudo
     CDS             3299..4056
                     /locus_tag="ACHKW0_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4102..5451
                     /locus_tag="ACHKW0_003890"
     CDS             4102..5451
                     /locus_tag="ACHKW0_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HFO7476643.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5451..6275
                     /locus_tag="ACHKW0_003891"
     CDS             5451..6275
                     /locus_tag="ACHKW0_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476644.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLIRKQTKGDELTTDERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSGFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPVALDSDIRQRLELLISRFPL"
     gene            6287..6847
                     /locus_tag="ACHKW0_003892"
     CDS             6287..6847
                     /locus_tag="ACHKW0_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HFO7476645.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6878..7948
                     /gene="lptF"
                     /locus_tag="ACHKW0_003893"
     CDS             6878..7948
                     /gene="lptF"
                     /locus_tag="ACHKW0_003893"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HFO7476646.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALYVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSVFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7945..9024
                     /gene="lptG"
                     /locus_tag="ACHKW0_003894"
     CDS             7945..9024
                     /gene="lptG"
                     /locus_tag="ACHKW0_003894"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HFO7476647.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9060..10232)
                     /locus_tag="ACHKW0_003895"
     CDS             complement(9060..10232)
                     /locus_tag="ACHKW0_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HFO7476648.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10232..10480)
                     /locus_tag="ACHKW0_003896"
     CDS             complement(10232..10480)
                     /locus_tag="ACHKW0_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HFO7476649.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10512..11426)
                     /locus_tag="ACHKW0_003897"
     CDS             complement(10512..11426)
                     /locus_tag="ACHKW0_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HFO7476650.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSKLVAGASAVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETIQTQTYELCDGVAGGYDW
                     QRIQQLAANVRCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            complement(11423..>13129)
                     /locus_tag="ACHKW0_003898"
     CDS             complement(11423..>13129)
                     /locus_tag="ACHKW0_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HFO7476651.1"
                     /translation="FTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRCQLEDQLEY
                     QTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAVPLAIPMGV
                     GQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWFKALPEADV
                     ALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPGDRCVSWLP
                     FYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVAPPFGYELC
                     QRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFMPCYGLAEN
                     ALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALPEHGIEIRN
                     EAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLLDGYLYVTG
                     RIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCRISDEERRG
                     QLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYAASLHVQES
                     LA"
BASE COUNT         3137 a         3257 c         3417 g         3318 t
ORIGIN      
        1 tttgaatgaa tgcttattgt ctgatacaca agaaataaca ctctttttat cgttaaaaaa
       61 tgatatttca ctttgcccat gccgttaaag tcactgataa tgcgtccgtt cgtaaattca
      121 aaatggcgta atctaatata tgctaaattt atttgttggc cttgatatat acacagggct
      181 tttgttattg cttgctctgg catttgtgtt gttctacgaa gcaatcaatg gttttcatga
      241 cacggcgaat gcggtggcaa ccgttattta tactcgtgcc atgcaaccac aacttgctgt
      301 ggtgatggcg gcatttttta acttttttgg cgtgttattg ggcggactta gcgttgccta
      361 tgccattgtc catatgttgc caaccgattt gttgctgaat atggggtcaa cccacggcct
      421 ggcgatggtc ttttccatgc tgctggcggc gattatctgg aacctgggaa cgtggttctt
      481 cggtttaccg gcctccagtt cgcacacctt gattggtgcg attatcggca tcggtttaac
      541 caacgcgctg ttaaccggct catcggtgat ggatgcgtta aacctgcgtg aagtgaccaa
      601 aattttctcc tcgctgattg tttcccctat cgtcggcctg gtcattgcgg gaggcctgat
      661 attcctgctg cgacgctact ggagcgggac gaaaaagcgt gaccgtattc accgcattcc
      721 ggaagatcgc aaaaagaaaa aaggcaaacg taaaccgcca ttctggacgc gtattgcgct
      781 gattgtttcc gctgcgggcg tggcgttttc gcacggcgcg aacgacggac agaaagggat
      841 cggcctggta atgctggtac tggtggggat tgcccctgct ggcttcgtcg tcaatatgaa
      901 tgcgtccggc tatgaaatta cccgtacccg cgatgccgtt accaacttcg aacactacct
      961 gcaacagcat cctgaactgc cgcagaagtt gattgcgatg gaacctccat tgcctgcagc
     1021 atcgactgat ggcacgcaag taacagagtt tcactgtcat ccggcaaata cctttgatgc
     1081 tattgcgcgc gttaaaacga tgctgccagg caatatggaa agttacgagc cgttaaccgt
     1141 gagtcagcgc agccagctgc gccgcattat gctgtgcatc tctgatacct ccgcgaagct
     1201 ggcgaaactg ccaggcgtca gtaaagaaga ccagaacctg ctgaaaaaac tgcgcagcga
     1261 tatgttaagc accattgagt acgctccggt gtggatcatc atggcggtag cactggcgct
     1321 cggcattggc accatgattg gctggcgtcg tgtagcgatg accatcggtg agaagattgg
     1381 taagcgcggc atgacgtatg cgcaaggcat ggcggcacaa atgacggcgg cagtgtctat
     1441 cggccttgcc agttatattg ggatgcctgt ctccacaaca cacgtcctct cgtctgcggt
     1501 agcagggacg atggtggtgg acggcggtgg gttacagcgt aaaacggtaa ccagcatcct
     1561 gatggcgtgg gtgtttactt taccggcggc aatttttctt tctggtgggc tgtactggat
     1621 agcattgcag ttgatttaac cttcctgaaa atgcccggtc ctggcgatcg ggcatttcca
     1681 tttttgacta gtgataacca cgcgcggtca taaaatccgt aatcgctttt tctacatcaa
     1741 ccaacacctg ttccagtggc tgattggcat cgatatcaac cagttgtgca ccaccaaagg
     1801 ttaactgtgg cgttatggca atcttcctcg ccagcgattc ccgtttatgg tcgggtttac
     1861 gtgcacaggc aacttcaagg tcaacattga gtttgatgac cagatcaggc ttatggctcg
     1921 ccatccagtg aaacgcttta cgttcctggc ttgccagcca tgagacaaaa cgaccacctt
     1981 caacgttagg tgggaacacc gtaccatcgt aagcgccagg aatttggtcc tgaggataac
     2041 ggtcggttag aacaattaac ccgcgacgac gacaggcaag catatgacga aagcgcagta
     2101 agcgacgggc gacaaacgct gtaattacca gcgccggaac tggtccaggc aatttttttg
     2161 ctgttttcac ctgatttcgt tcaattgttt tatgtaagga ttttcccatc aacggtaatt
     2221 ttgtcactgc acgaccgaca tttccggcct gttttcctaa atgaactctt tcggcagcac
     2281 catatttttc gacaacggta ataagatgtt cacacaccgt tgacttgcct gaaccatcgc
     2341 taccaataac ggcaattaat ggaggtgtta ttgactgcat gaatatatcc tttaatttaa
     2401 atatccatta aaaatattta tttggttaat atgtttttat gaaagcgtaa ttcaggtcaa
     2461 tgtcacaatt aaccattgtc acaataaggt tgaacggata ttctgagcac ggaccacgta
     2521 atattcaata cattatttac tgccgggttt ttcgtgaatc aacgtaatat gtcaataatt
     2581 aattccacgc cagtgcgtgt tattgccatt gtaggatgtg atggttcagg taaatcgacc
     2641 ctcacggcaa gcctggtaaa tgaactggca gcaagaatgc caacagaaca catttatctc
     2701 gggcaatcgt ccgggcgaat tggcgaatgg atttcacagc tccctgttat tggcgcacct
     2761 tgtgggcgtt atctgcgaag taaagcggca catgtgcacg aaaaaccctc aacaccgcct
     2821 ggcaatatta ctgcgctggt tatctatctg ctttcctgct ggcgggcgta caagtttcgc
     2881 aaaatgttgt gtaaaagcca gcaaggcttt ctgctcatca ccgaccgcta cccgcaggtt
     2941 gaagtgccgg gttttcgctt tgatggcccg caattggcaa aaaccacggg cggtaacggt
     3001 tggataaaaa tgttaaggca gcgcgagctg aagctgtacc aatggatggc atcttatttg
     3061 cccgtattgt tgattcgtct tggcattgat gaacaaaccg cgtttgcgcg taaacctgac
     3121 caccaactgg cagcgttaca ggaaaaaatc gccgttacgc cgcaactgac attcaatggc
     3181 gcaaagatcc ttgaactgga tgggcgacac cccgccgatg aaattctgca agcgtcacta
     3241 cgcgcaattc acgccgcgct ttcctgatca ccaaaatgga taggggatga tttaacgaat
     3301 ggatgcacta caaactcaaa ctgttaatag tacaaccgcg ccgcagccca actacattcc
     3361 ggggctgatt gcggtggtcg ggtgtgatgg caccggtaaa tccacactga ccaccgacct
     3421 ggtgaaatcg ctgcaacaac actggcaaac cgagcggcgc tatctggggc tgctctccgg
     3481 cgaagacggc gacaaaatca aacgattgcc gttggttggc gtctggctgg aacggcgact
     3541 ggcggccaaa tcctcgaaaa cccaaagcat gaaaaccaaa tctccggcgc tatgggcggc
     3601 ggtgattatg tactgcttct cgctgcgaag aatggcgaat ctacgcaagg ttcagcgact
     3661 ggcgcaaagt ggcgttctgg tggtcagcga tcgcttcccg caggctgaaa tttcgggctt
     3721 ttattatgat ggaccgggga ttggcgtcga acgtgcgacc gggaaaatca gcatgtttct
     3781 ggcgcagcgc gaacggcgtt tataccaaca aatggcgcaa tatcgcccgg aattaattat
     3841 tcgcctgggc attgatattg agactgccat ctcccgcaag cctgaccatg actatgccga
     3901 gctgcaggac aaatcggcgt catgtcgacg attggctata acggcacaaa aattcttgag
     3961 attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc ggtttctctg
     4021 gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac tttttactat
     4081 caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt tgcagatggc
     4141 gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt cgtgagcgcc
     4201 ttgctgggtc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc catgtttggc
     4261 gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa gtttcagaca
     4321 tggcaactgg tggttcagta cggaacgcca gccttgacca acaataatcc gcagcaattt
     4381 cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt ggcgattgtc
     4441 ggtggcattg ccttactgcc attcctttcc cattcattag gtctggatga ccaaagcttt
     4501 tggctggcgg cgctctattg cacgctcatt ccttcaatgg cttcctccac gccgaccggc
     4561 attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac gaaacctttt
     4621 ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc gggttttgtt
     4681 attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt tgcagcgcgc
     4741 gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga gtctgcccga
     4801 cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc catctggtcg
     4861 gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc tgccgccggg
     4921 ttatttaaaa tcgccatgac attcttcgac gccgccggaa cgccagcggg tttgctgggt
     4981 aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc gtggttgtta
     5041 ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct cgcggtgttg
     5101 atcgtcggca agccgctcat ttcgcttgtg tttggcgtta agtatctcga agcgtatgac
     5161 ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc gcaggagtca
     5221 ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat cgcctcaatc
     5281 ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg agcagcgttt
     5341 gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac tttaaaggca
     5401 ttttttcagc gtcattcctt gctttataac gcagccggag agaaatcctg atgatgagaa
     5461 aatatttccc gcttgaggcg agcgagcgtc tatttgttgc gattgaggaa gatgatgtcg
     5521 ttgatgccca ggtcagtttg ccgccgacta ttgcactaag ttgtaccacc gaaatcatcc
     5581 atgataatta cgcgttatgc ctgcagttct ggctcaatgg cgttaatcgt caggagttac
     5641 tgcgtttaat tcgcaaacag accaaaggcg atgagctgac gacagacgag cgcaaacagt
     5701 ttaaatacat gcgagcgcgc tacaagcacc tgcgttttgc gcaacggctg tatttaaaga
     5761 agcatcaggc cggattttta ttcggcaaaa cgaccgtttt tctggggcgt tttcaggacg
     5821 gctttcgtaa cggtaagaaa aatattgtgt cgtattacgg caacctgtta cggatttatt
     5881 taagctctcc ggtctggtcg ttggtgaact attcattacg ccatagccag ttagaaagcg
     5941 taagcggttt tattgcctat cggcaaaagc aaatgcatac cctgaaagag attattgcca
     6001 aaccgcgact aaccggtagg gaatttcatg atgtgcgaaa aatcatcagc cagcaggttt
     6061 cttattacga caccttaaga tcgcttgatc ctgaaaataa agaggcgtta cagatatccc
     6121 gcttcctcgc cgccattaat ggcctgatgg gagataaaca cgacgacatg gtggcggatg
     6181 atatggaaaa ccgccagtcg tatgatgcac cggtggcgct ggatagcgat attcgccagc
     6241 gcttagagct gctgatttcg cgttttccgc tataaccgga gcaatgatgt catctcgttt
     6301 gattattgcg ctgattatca tgctgttagc cccgggtgtg caggcacaca atttcgtcac
     6361 tggcaaaacg gttacccctg tttatatcca ggaaggcggc gagctgcttc tgaatagcga
     6421 cgacgaaatt cactaccaaa agtggaacag cacacaattg gcgggaaaag tccgcattat
     6481 ccagtatatc gccgggcgta agtcggcgaa gaaaaagaac tcactgttaa ttaaagcggt
     6541 tgaagcggca aactttccgc aggatcgttt tcagcccacc accattgtga acaccgatga
     6601 tgccatcttt ggtactggct attttgttgt tggcaaaatc gaaaaaaata aacgccgcta
     6661 tccgtgggcg caattcgtta ttgacggcaa tggacaggga cgcgttgcct ggcgcttgcc
     6721 ggagcagagt tcaaccattc tggtgctgaa taaagccggg caaattcagt gggcaaagga
     6781 cggcagcctg acgccggaag aggtagatca tgtcattgcg ttggcgcaga aactgatcaa
     6841 tgaataaaaa actcaaagga ttgagggcgt tagtgagatg aagctggttg aacactacat
     6901 catgcgtggt acgcgccgtc tggtgctgat tatcgtcggt tttctgatct ttattttcgc
     6961 cagctactcc gcacagcgtt atctgaccga agcggcaaat gggacattag ctctttatgt
     7021 tgtgctggat atcgttttct acaaagtact gattgcactg gagatgttgt tacctgttgg
     7081 tctgtatgtg tcagttggcg tgacgctagg gcagatgtat accgactcgg aaattaccgc
     7141 tatctctgcg gcggggggca gtccgggacg cttgtacaaa gccgttcttt atctggcgat
     7201 accgctaagt atttttgtca cccttctgtc gatgtatggt cgtccgtggg cttatgcgca
     7261 gatttatcaa ctggagcaac agtcgcagtc ggagctggat gttcgccagt tgcgggcaaa
     7321 gaaatttaac actaacgata acggacgaat gatcctttcg cagacggttg atcaggataa
     7381 taatcgcctg actgacgcgc tgatttatac ttctactgcc aatcgaaccc gtattttccg
     7441 cgcccgttcg gttgatgtgg ttgacccatc acccgagaaa ccgaccgtta tgttgcacaa
     7501 cggcactgcc tatcttctcg atcatcaagg gcgtgacgac aacgaacaga tctaccgtaa
     7561 tctgcaatta catctgaatc cgctggatca aagcccgaac gtcaaacgca aagcgaaatc
     7621 ggtcacggag ctggcgcgct ccgtctttcc tgccgatcat gccgaactgc aatggcgaca
     7681 aagccgtggt ctgacagcat tgttgatggc gctgctggcc atttcattaa gtcgggtaaa
     7741 accgcggcaa gggcgatttt caacgttatt gccactgacg ttgctgttta ttgccatttt
     7801 ttatggcggc gacgtctgcc gtacgctggt ggctaacggc gcgattcccc tcattcctgg
     7861 tttgtggtta gtgcccggac tcatgctaat gggcctgctg atgctggtcg cacgcgactt
     7921 ctctttgctg cagaaatttt cccgatgaat gttttcagtc gctatttaat ccgtcatctc
     7981 tttctcggtt ttgccgccgc cgcagggcta ttgctgccgc tttttaccac cttcaacttg
     8041 attaacgaac tggatgatgt cagcccgggc ggttatcgct ggactcaggc ggtgctggtg
     8101 gtgctaatga ccttaccgcg cacactggtc gaactttcgc cgtttatcgc gttattgggt
     8161 ggtattgttg gactggggca gttatcgaaa aacagtgagc ttaccgccat tcgcagcacg
     8221 gggttttcta tcttccgtat tgcactggtg gcgctggttg ccgggatatt gtggactgtt
     8281 tcgttaggcg cgatagatga gtgggtggcg tcgccattgc agcaacaggc gttgcaaatc
     8341 aaatcgaccg ccaccgcgtt gggggaggac gatgacatta ccggcaatat gctgtgggcc
     8401 aggcgcggta atgaatttgt gacggtgaaa tcgctgaacg agcagggcca gccggtgggc
     8461 gtggagatat ttcattatcg cgacgatctc tcgctcgaat cttacattta tgcacgcagt
     8521 gccaccattg aagacgacaa aacgtggatc ctgcatggtg tgaatcataa aaaatggctt
     8581 aacggtaaag aaacgctgga aacatcagat aatcttgcct ggcaatcggc cttcaccagt
     8641 atggatcttg atgagttatc gatgccgggg aatacttttt ctgtccgtca gcttaatcat
     8701 tacatccatt atttgcagga aaccgggcaa cccagcagcg aataccgcct tgcactgtgg
     8761 gaaaaactgg ggcaaccgat cctgaccctg gcgatgattt tgctggctgt accgtttacc
     8821 tttagcgccc cgcgctcgcc agggatgggt agccgtctcg ctgtaggcgt catcgtgggc
     8881 ttactcacct ggatcagcta tcaaatcatg gttaatctgg gattgctatt tgcgttaagc
     8941 gcacctgtta ccgcgctcgg tttaccggta gcgtttgtgt tggtggcgtt gagcctggtg
     9001 tattggtatg acagacaaca ttaaacccgc cgtggcgcaa acaccacggc gggctcacat
     9061 tactgcttca gagtagcaaa tgcctgaatg atggcatcga tttgctcacg actgtgggca
     9121 gcatttacgc tacagcgcag cagcgtgatg cctgcaggcg cggcaggcgg caggataaga
     9181 ttcacataca cgcccagcga gataagctcg cgccagatgc gtaaaccttc ctctttcgaa
     9241 ccgattatta ctggcactac cgggctaata tgtgaactta actcataacc cagtttttgc
     9301 agaccgtcat acagatggtt ggcgttatcc atcagctttt gccgcagctc tggttgtgtg
     9361 gcaatagttc ttaacgacga gcgcacggtc gcaatgcagg acggagaagg agaggcggta
     9421 aaaatatacg ggcggctgcc gtagcgcagc acttccatcg cttccgaacc gacagcaaaa
     9481 ccgccaatcg aggccagact tttactgaaa gtaccaacga taatatccac atcatcttca
     9541 acgccgacag cttcagccag tccgcgcccc gttgcgccta acacgccaaa cgaatgcgct
     9601 tcatcgacaa tcaaataacc accgagacga cgtttgatat caacaatttc cgccagcggc
     9661 gcaacgtcac cgagcatgct gtagatgccc tcaacgatga taatcgcctc tttcgcccgt
     9721 tcaccgaggc gaaccatgcg ccgttccaga tctttagcat cgttatggcg gaaacgaata
     9781 atttccgcgc caccgagaga acaggcgtca taaatactgg cgtggctgtc ggcatccagt
     9841 aacaccacgg cgttatggtc ggcaagtgcg ctgatgacgc ctaaattagc ggtataaccg
     9901 gttgaaaaaa cgatagcggt agggcgattg aaaaactcgg caatctcttt ctctaacgcc
     9961 aggtgcggcg cataactgcc gtttgccata cgagaaccgg tggtgccggt gccttgggcc
    10021 gctaacgctg cctgaccttc gctgatggca tcatgattaa aggttaaacc gagataatta
    10081 ttggtcccgg ccagaatcac ttttttattg ccgatccggc cttcggtggc ggaatacact
    10141 tcatcaatac aggtgccaaa cggtgttaac ccgttgtcac taaattgcag tcgctcgcca
    10201 gcaaggcgcg catatttatc gtatagcccc attatttatt ctccagccat ggaagtagag
    10261 tttccattaa ttgcgcaggg gttttgacat ccagcagaat attaatagga atggaaatat
    10321 cgaatctgtc ttccagcatc attaagagat ccatcacttt gatcgactcc aggccaagat
    10381 cgttgacgag atcgctgtcg ggcttaattt ccacaccgtt ttcgaccaaa tcctgtaaac
    10441 aggaaaggat gtaatccatt actatttcac gatttaccat agaaacgtta acacaccttt
    10501 aaacaagaca atcaaaatag cccgttgcgt aatgcgtgtt ccaggctaat cccgggaaac
    10561 caattaatat cttcagaaat acggttatta cttgccgacc agtcggcgtg ggttaattca
    10621 cgaattttcg agcgggtcag cataggttct ttacctgcca ggcgactcaa cgcggtactg
    10681 atatccgcaa ggcaggtgag taccggcaga ggaataccaa ccattcgcac ggaaccgcaa
    10741 cggacgttgg cggcaagttg ctgtatgcgt tgccagtcat agccgccagc gacgccatcg
    10801 cataattcat aggtttgcgt ctgtatagtt tcggcgctta accactgacc cactgcttgc
    10861 gcgaaatcgg tgacgtgcag aaaagagagc tgtgtatctg gcgcaccaag tcgtggcagc
    10921 aggccgcgca gcatccagtc aaacagcggt tttaactctt tatcgccggg accatatacg
    10981 gctgtcgggc gaaaaacgcc cagcgtaatt tcgtcagcca ttgcagtcag ccgttgttcg
    11041 gcgacgtgtt tggaatttgc gtaccaggag agttcgggat ggcgcgccgc cagcgaagag
    11101 ataaacagaa aacgttggca aaaaccgctc tcttttgctg cttgcatcag gcgcaggctg
    11161 ccgtcaacgt tacagcgggt gaaaatctct tctttgtgcc cgcgcacttg cccggcgcaa
    11221 tggaccaccg cgctggctcc ggcaaccagc ttgctaagtg aatgagtatc ttccagcgaa
    11281 ccgcgcaccc aggtgagatt atcattgacg tgagcgcggg cagtacgcgt caatgcgcga
    11341 acgtgaaagc cgcgggcgag caggttatcg ataatatatt taccgataaa cccggtagcg
    11401 cccgtcaccg cgacagtttg attcatgcca gggattcctg cacatgaagg cttgcagcat
    11461 aagccttctg ataacgtttt ttcgcttccg cacgggcagg cttgccggag gatgttcggg
    11521 gaatactgtg gggcggcaac agatcgatag ccgctgttac gccaaattcg ctttggatcc
    11581 gtgctgccag cgcgtggata agctgcccgc gacgttcttc gtcgctgatc cgacactgga
    11641 tctgcaaaat gattttttcc tgggcggtaa caaaagcaat cgcatcgcca gaatgaattt
    11701 ccggttcttg ttccgcaata tattcaatat cctgcggcca gatattacgg ccacgaataa
    11761 taatcagatc tttaatacgt ccggtgacat acagataacc gtccagcaga taaccgaggt
    11821 cgccggtgtc taaccagccc gtcgcggcaa tctcgtcttg cgaaacctgg tcgccaaagt
    11881 aaccgctcat caggctgggg ccggagatgc aaatatggcc taccacacgt tccgcgaccg
    11941 gcatacctgc ttcattgcgg atttcaatgc catgttccgg caacgctttg ccgcagttga
    12001 cgaaagtcga tacggcgcgt gtttcagcac ccggcgcgac ggctttgccc tgatattcta
    12061 ggatgtcgcg atccacttcg ttaaccacaa ccccagagcc ttcatcagag aagctgacag
    12121 ccagcgcatt ttctgccagt ccgtagcacg gcatgaacgt tttatcgtca aagttaacct
    12181 gacggaaaca ttcagcgaat tgatggagtt gttctgcgga gatcggctca gcaccaatac
    12241 cagcgacgcg ccagcaggaa agatccagtt cagcgagatc tttttcattc acgcggcgct
    12301 ggcacaattc atagccaaac ggcggcgcaa cggaaacggt gccgcgattt ttactgatca
    12361 atttaagcca ttgcagagga cgcatggcaa aatcctgagt gcgcaaataa tctactgaaa
    12421 gttgcgtggc gacgggggtc aggagaaagc cgaccagtcc catatcatgg tagaaaggca
    12481 gccaggagac gcagcggtcg ccagggcgta atttaatgcc atcgtggctt atagcacgta
    12541 gattagccat cacttcgcga tgggtgataa tgacgccacg gggaaaacgg gtgctgccgg
    12601 aggtgtactg gaggtaggcg atatcgttcg gaactggacg ctggagcgca acatcggctt
    12661 ccggtaatgc cttaaaccag gcgtggctta aaacatgtaa ttcggggttg ttatgcgtcg
    12721 cggcattgac cagtggcaac cactcatcac cagtgataat ggctgcgggc tggcaacttg
    12781 ccagtaaacc ctggagtttg gcgctccagg aatcccgctg accgacgccc attggaatcg
    12841 ccaacgggac ggcgactaag ccagcatact gacaggcaaa aaaagcctct acaaacccgc
    12901 tactcgtttc ggcaatcagc gccacgcgtt cgcctttttt caggttcagc gataacaacc
    12961 gcttcgcacc agcttcggca cgcgttttta acgtctgata ttccagttga tcttcaagtt
    13021 ggcaacgtct gtcataaaaa ttcattccgg cgctactcag agcggcgtag tccaaagcat
    13081 caaccagcgt tggaaaatcg gcatagcgca taggtaggga atgcgtaaa
//
LOCUS       DAZFXT010000125        12811 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.125, whole genome shotgun sequence.
ACCESSION   DAZFXT010000125 DAZFXT010000000
VERSION     DAZFXT010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12811
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.125"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            144..689
                     /locus_tag="ACHKW0_003899"
     CDS             144..689
                     /locus_tag="ACHKW0_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HFO7476652.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASEADLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGLPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            664..2589
                     /locus_tag="ACHKW0_003900"
     CDS             664..2589
                     /locus_tag="ACHKW0_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7476653.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFTNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDDDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQLDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDTGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            2586..2792
                     /locus_tag="ACHKW0_003901"
     CDS             2586..2792
                     /locus_tag="ACHKW0_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019058 - viral life cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail joining protein"
                     /protein_id="HFO7476654.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            2789..4390
                     /locus_tag="ACHKW0_003902"
     CDS             2789..4390
                     /locus_tag="ACHKW0_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7476655.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRSWNPPSES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     EARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSQEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFVQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            4371..5690
                     /locus_tag="ACHKW0_003903"
     CDS             4371..5690
                     /locus_tag="ACHKW0_003903"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7476656.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISRLT
                     DAVSGDSLTAQEALATLALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTDVVPAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVKTAR
                     RILAAAPQSAQARSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            5700..6032
                     /locus_tag="ACHKW0_003904"
     CDS             5700..6032
                     /locus_tag="ACHKW0_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7476657.1"
                     /translation="MTSKETFTHYQPQGNSDPAHTATAPGGLSAKAPAMTPLMLDTSS
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTAF
                     AGTAISIV"
     gene            6088..7113
                     /locus_tag="ACHKW0_003905"
     CDS             6088..7113
                     /locus_tag="ACHKW0_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7476658.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSEEN
                     NITQSGGTEWSQRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSNSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7155..7553
                     /locus_tag="ACHKW0_003906"
     CDS             7155..7553
                     /locus_tag="ACHKW0_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7476659.1"
                     /translation="MTKDELIARLRSLGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVGSAQPDTVILDTSELVTVVALVKLHTDALHATRDE
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            7565..7918
                     /locus_tag="ACHKW0_003907"
     CDS             7565..7918
                     /locus_tag="ACHKW0_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7476660.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATITSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            7930..8508
                     /locus_tag="ACHKW0_003908"
     CDS             7930..8508
                     /locus_tag="ACHKW0_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7476661.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRRKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            8505..8900
                     /locus_tag="ACHKW0_003909"
     CDS             8505..8900
                     /locus_tag="ACHKW0_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7476662.1"
                     /translation="MKHTELRAAVLDALEKHDTGATFFDGRPAVFDEADFPAVAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDAWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVITYEM"
     gene            8908..9648
                     /locus_tag="ACHKW0_003910"
     CDS             8908..9648
                     /locus_tag="ACHKW0_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7476663.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     ATGMTVTPSSTSVVKGQSTTLTVAFQPEGVTDKSFRAVSADKTKATVSVSGMTITVNG
                     VAAGKVNIPVVSGNGEFAAVAEITVTAS"
     gene            9664..10086
                     /locus_tag="ACHKW0_003911"
     CDS             9664..10086
                     /locus_tag="ACHKW0_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7476664.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLALMKRQAEQAESDSN
                     RKFTVEDAIRTGAFLVAMSLWHNHPQKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNNAPEQTEDAGPAEPVSAGKCSTVS"
     gene            10068..10502
                     /locus_tag="ACHKW0_003912"
     CDS             10068..10502
                     /locus_tag="ACHKW0_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7476665.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPDMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            10495..12811
                     /locus_tag="ACHKW0_003913"
                     /pseudo
     CDS             10495..12811
                     /locus_tag="ACHKW0_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
BASE COUNT         2945 a         3278 c         4014 g         2574 t
ORIGIN      
        1 ttaaggcgtt tccgttcttc ttcgtcgtaa cttaatgttt ttatttaaaa taccccctga
       61 aaagaaagga aacgacaggt gctgaaaacg aacttttggg cctttgtcgt ttcctttctc
      121 tgtttttggc cgtggaatga acaatggaag tcaacaaaaa gcagctggct gacattttcg
      181 gtgcgagtat ccgtaccatt cagaactggc aggaacaggg aatgcccgtt ctgcgaggcg
      241 gtggcaaggg taatgaggtg ctttatgact ctgccgccgt cataaaatgg tatgccgaaa
      301 gggatgctga aattgagaac gaaaagctgc gccgggaggt tgaagaactg cggcaggcca
      361 gcgaggcaga tctccagcca ggaactattg agtacgaacg ccatcgactt acgcgtgcgc
      421 aggccgacgc acaggaactg aagaatgcca gagactccgc tgaagtggtg gaaaccgcat
      481 tctgtacttt cgtgttgtcg cggatcgcag gtgaaattgc cagtattctc gacgggctcc
      541 ccctgtcggt gcagcggcgt tttccggaac tggaaaaccg acatgttgat ttcctgaaac
      601 gggatatcat caaagccatg aacaaagcag ccgcgctgga tgaactgata ccggggttgc
      661 tgagtgaata tatcgaacag tcaggttaac aggctgcggc attttgtccg cgccgggctt
      721 cgctcactgt tcaggccgga gccacagacc gccgttgaat gggcggatgc taattactat
      781 ctcccgaaag aatccgcata ccaggaaggg cgctgggaaa cactgccctt tcagcgggcc
      841 atcatgaatg cgatgggcag cgactacatc cgtgaggtga atgtggtgaa gtctgcccgt
      901 gtcggttatt ccaaaatgct gctgggtgtt tatgcctact ttatagagca taagcagcgc
      961 aacaccctta tctggttgcc gacggatggt gatgccgaga actttatgaa aacccacgtt
     1021 gagccgacta ttcgtgatat tccgtcgctg ctggcgctgg ccccgtggta tggcaaaaag
     1081 caccgggata acacgctcac catgaagcgt ttcactaatg ggcgtggctt ctggtgcctg
     1141 ggcggtaaag cggcaaaaaa ctaccgtgaa aagtcggtgg atgtggcggg ttatgatgaa
     1201 cttgctgctt ttgatgatga tattgaacag gaaggctctc cgacgttcct gggtgacaag
     1261 cgtattgaag gctcggtctg gccaaagtcc atccgtggct ccacgccaaa agtgagaggc
     1321 acctgtcaga ttgagcgtgc agccagtgaa tccccgcatt ttatgcgttt tcatgttgcc
     1381 tgcccgcatt gcggggagga gcagtatctt aaatttggcg acaaagagac gccgtttggc
     1441 ctcaaatgga cgccggatga cccctccagc gtgttttatc tctgcgagca taatgcctgc
     1501 gtcatccgcc agcaggagct ggactttact gatgcccgtt atatctgcga aaagaccggg
     1561 atctggaccc gtgatggcat tctctggttt tcgtcatccg gtgaagagat tgagccacct
     1621 gacagtgtga cctttcacat ctggacagcg tacagcccgt tcaccacctg ggtgcagatt
     1681 gtcaaagact ggatgaaaac gaaaggggat acgggaaaac gtaaaacctt cgtaaacacc
     1741 acgctcggtg agacgtggga ggcgaaaatt ggcgaacgtc cggatgctga agtgatggca
     1801 gagcggaaag agcattattc agcgcccgtt cctgaccgtg tggcttacct gaccgccggt
     1861 atcgactccc agctggaccg ctacgaaatg cgcgtatggg gatgggggcc gggtgaggaa
     1921 agctggctga ttgaccggca gattattatg ggccgccacg acgatgaaca gacgctgctg
     1981 cgtgtggatg aggccatcaa taaaacctat acccgccgga atggtgcaga aatgtcgata
     2041 tcccgtatct gctgggatac tggcgggatt gacccgacca ttgtgtatga acgctcgaaa
     2101 aaacatgggc tgttccgggt gatccccatt aaaggggcat ccgtctacgg aaagccggtg
     2161 gccagcatgc cacgtaagcg aaacaaaaac ggggtttacc ttaccgaaat cggtacggat
     2221 accgcgaaag agcagattta taaccgcttc acactgacgc cggaagggga tgaaccgctt
     2281 cccggtgccg ttcacttccc gaataacccg gatatttttg atctgaccga agcgcagcag
     2341 ctgacggctg aagagcaggt cgaaaaatgg gtggatggca ggaaaaaaat actgtgggac
     2401 agcaaaaagc gacgcaatga ggcactcgac tgcttcgttt atgcgctggc ggcgctgcgc
     2461 atcagtattt cccgctggca gctggatctc agtgcgctgc tggcgagcct gcaggaagag
     2521 gatggtgcag caaccaacaa gaaaacactg gcagattacg cccgtgcctt atccggagag
     2581 gatgaatgac gcgacaggaa gaacttgccg ctgcccgtgc ggcactgcat gacctgatga
     2641 caggtaaacg ggtggcaaca gtacagaaag acggacgaag ggtggagttt acggccactt
     2701 ccgtgtctga cctgaaaaaa tatattgcag agctggaagt gcagaccggc atgacacagc
     2761 gacgcagggg acctgcagga ttttatgtat gaaaacgccc accattccca cccttctggg
     2821 gccggacggc atgacatcgc tgcgcgaata tgccggttat cacggcggtg gcagcggatt
     2881 tggagggcag ttgcggtcgt ggaacccacc gagtgaaagt gtggatgcag ccctgttgcc
     2941 caactttacc cgtggcaatg cccgcgcaga cgatctggta cgcaataacg gctatgccgc
     3001 caacgccatc cagctgcatc aggatcatat cgtcgggtct tttttccggc tcagtcatcg
     3061 cccaagctgg cgctatctgg gcatcgggga ggaagaagcc cgtgcctttt cccgcgaggt
     3121 tgaagcggca tggaaagagt ttgccgagga tgactgctgc tgcattgacg ttgagcgaaa
     3181 acgcacgttt accatgatga ttcgggaagg tgtggccatg cacgccttta acggtgaact
     3241 gttcgttcag gccacctggg ataccagttc gtcgcggctt ttccggacac agttccggat
     3301 ggtcagcccg aagcgcatca gcaacccgaa caataccggc gacagccgga actgccgtgc
     3361 cggtgtgcag attaatgaca gcggtgcggc gctgggatat tacgtcagcg aggacgggta
     3421 tcctggctgg atgccgcaga aatggacatg gataccccgt gagttacccg gcgggcgcgc
     3481 ctcgttcatt cacgtttttg aacccgtgga ggacgggcag actcgcggtg caaatgtgtt
     3541 ttacagcgtg atggagcaga tgaagatgct cgacacgctg cagaacacgc agctgcagag
     3601 cgccattgtg aaggcgatgt atgccgccac cattgagagt gagctggata cgcagtcagc
     3661 gatggatttt attctgggcg cgaacagtca ggagcagcgg gaaaggctga ccggctggat
     3721 tggtgaaatt gccgcgtatt acgccgcagc gccggtccgg ctgggaggcg caaaagtacc
     3781 gcacctgatg ccgggtgact cactgaacct gcagacggct caggatacgg ataacggcta
     3841 ctccgtgttt gagcagtcac tgctgcggta tatcgctgcc gggctgggtg tctcgtatga
     3901 gcagctttcc cggaattacg cccagatgag ctactccacg gcacgggcca gtgcgaacga
     3961 gtcgtgggcg tactttatgg ggcggcgaaa attcgtcgca tcccgtcagg cgagccagat
     4021 gtttctgtgc tggctggaag aggccatcgt tcgccgcgtg gtgacgttac cttcaaaagc
     4081 gcgcttcagt tttcaggaag cccgcagtgc ctgggggaac tgcgactgga taggctccgg
     4141 tcgtatggcc atcgatggtc tgaaagaagt tcaggaagcg gtgatgctga tagaagccgg
     4201 actgagtacc tacgagaaag agtgcgcaaa acgcggtgac gactatcagg aaatttttgt
     4261 ccagcaggtc cgtgaaacga tggagcgccg tgcagccggt cttaaaccgc ccgcctgggc
     4321 ggctgcagca tttgaatccg ggctgcgaca atcaacagag gaggagaaga gtgacagcag
     4381 agctgcgtaa tctcccgcat attgccagca tggcctttaa tgagccgctg atgcttgaac
     4441 ccgcctatgc gcgggttttc ttttgtgcgc ttgcaggcca gcttgggatc agccgcctga
     4501 cggatgcggt gtccggcgac agcctgactg cccaggaggc actcgcgacg ctggcattat
     4561 ccggtgatga tgacggacca cgacaggccc gcagttatca ggtcatgaac ggcatcgccg
     4621 tgctgccggt gtccggcacg ctggtcagcc ggacgcgggc gctgcagccg tactcgggga
     4681 tgaccggtta caacggcatt atcgcccgtc tgcaacaggc tgccagcgat ccgatggtgg
     4741 acggcattct gctcgatatg gacacgcccg gcgggatggt ggcgggggca tttgactgcg
     4801 ctgacatcat cgcccgtgtg cgtgacataa aaccggtatg ggcgcttgcc aacgacatga
     4861 actgcagtgc aggtcagttg cttgccagtg ccgcctcccg gcgtctggtc acgcagaccg
     4921 cccggacagg ctccatcggc gtcatgatgg ctcacagtaa ttacggtgct gcgctggaga
     4981 aacagggtgt ggaaatcacg ctgatttaca gcggcagcca taaggtggat ggcaacccct
     5041 acagccatct tccggatgac gtccgggaga cactgcagtc ccggatggac gcaacccgcc
     5101 ggatgtttgc gcagaaggtg tcggcatata ccggcctgtc cgtgcaggct gtgctggata
     5161 ccgaggctgc agtgtacagc ggtcaggagg ccattgatgc cggactggct gatgaacttg
     5221 ttaacagcac cgatgcgatc accgtcatgc gtgatgcact ggatgcacgt aaatcccgtc
     5281 tctcaggagg gcgaatgacc aaagagactc aatcaacaac tgtttcagcc actgcttcgc
     5341 aggctgacgt tactgacgtg gtgccagcga cggagggcga gaacgccagc gcggcgcagc
     5401 cggacgtgaa cgcgcagatc accgcagcgg ttgcggcaga aaacagccgc attatgggga
     5461 tcctcaactg tgaggaggct cacggacgcg aagaacaggc acgcgtgctg gcagaaaccc
     5521 ccggtatgac cgtgaaaacg gcccgccgca ttctggccgc agcaccacag agtgcacagg
     5581 cgcgcagtga cactgcgctg gatcgtctga tgcagggggc accggcaccg ctggctgcag
     5641 gtaacccggc atctgatgcc gttaacgatt tgctgaacac accagtgtaa gggatgttta
     5701 tgacgagcaa agaaaccttt acccattacc agccgcaggg caacagtgac ccggctcata
     5761 ccgcaaccgc gcccggcgga ttgagtgcga aagcgcctgc aatgaccccg ctgatgctgg
     5821 acacctccag ccgtaagctg gttgcgtggg atggcaccac cgacggtgct gccgttggca
     5881 ttcttgcggt tgctgctgac cagaccagca ccacgctgac gttctacaag tccggcacgt
     5941 tccgttatga ggatgtgctc tggccggagg ctgccagcga cgagacgaaa aaacggaccg
     6001 cgtttgccgg aacggcaatc agcatcgttt aactttaccc ttcatcacta aaggccgcct
     6061 gtgcggcttt ttttacggga tttttttatg tcgatgtaca caaccgccca actgctggcg
     6121 gcaaatgagc agaaatttaa gtttgatccg ctgtttctgc gtctcttttt ccgtgagagc
     6181 tatcccttca ccacggagaa agtctatctc tcacaaattc cgggactggt aaacatggcg
     6241 ctgtacgttt cgccgattgt ttccggtgag gttatccgtt cccgtggcgg ctccacctct
     6301 gaatttacgc cgggatatgt caagccgaag catgaagtga atccgcagat gaccctgcgt
     6361 cgcctgccgg atgaagatcc gcagaatctg gcggacccgg cttaccgccg ccgtcgcatc
     6421 atcatgcaga acatgcgtga cgaagagctg gccattgctc aggtcgaaga gatgcaggca
     6481 gtttctgccg tgcttaaggg caaatacacc atgaccggtg aagccttcga tccggttgag
     6541 gtggatatgg gccgcagtga ggagaataac atcacgcagt ccggcggcac ggagtggagc
     6601 cagcgtgaca agtccacgta tgacccgacc gacgatatcg aagcctacgc gctgaacgcc
     6661 agcggtgtgg tgaatatcat cgtgttcgat ccgaaaggct gggcgctgtt ccgttccttc
     6721 aaagccgtca aggagaagct ggatacccgt cgtggctcta attccgagct ggagacagcg
     6781 gtgaaagacc taggtaaagc ggtgtcctat aaggggatgt atggcgatgt ggccatcgtc
     6841 gtgtattccg gacagtacgt ggaaaacggc gtcaaaaaga acttcctgcc ggacaacacg
     6901 atggtgctgg ggaacactca ggcacgcggt ctgcgcacct atggctgcat tcaggatgcg
     6961 gacgcacagc gcgaaggcat taacgcctct gcccgttacc cgaaaaactg ggtgaccacc
     7021 ggcgatccgg cgcgtgagtt caccatgatt cagtcagcac cgctgatgct gctggctgac
     7081 cctgatgagt tcgtgtccgt acaactggcg taatcatggc ccttcggggc cattgtttct
     7141 ctgtggagga gtccatgacg aaagatgaac tgattgcccg tctccgctcg ctgggtgaac
     7201 aactgaaccg tgatgtcagc ctgacgggga cgaaagaaga actggcgctc cgtgtggcag
     7261 agctggaaga ggagcttgat gacacggatg aaactgccgg tcaggacacc cctctcagcc
     7321 gggaaaatgt gctgaccgga catgaaaatg aggtgggatc agcgcagccg gataccgtga
     7381 ttctggatac gtctgaactg gtcacggtcg tggcactggt gaagctgcat actgatgcac
     7441 ttcacgccac gcgggatgaa cctgtggcat ttgtgctgcc gggaacggcg tttcgtgtct
     7501 ctgccggtgt ggcagccgaa atgacagagc gcggcctggc cagaatgcaa taacgggagg
     7561 cgctgtggct gatttcgata acctgttcga tgctgccatt gcccgcgccg atgaaacgat
     7621 acgcgggtac atgggaacgt cagccaccat tacatccggt gagcagtcag gtgcggtgat
     7681 acgtggtgtt tttgatgacc ctgaaaatat cagctatgcc ggacagggcg tgcgcgttga
     7741 aggctccagc ccgtccctgt ttgtccggac tgatgaggtg cggcagctgc ggcgtggaga
     7801 cacgctgacc atcggtgagg aaaatttctg ggtagatcgg gtttcgccgg atgatggcgg
     7861 aagttgtcat ctctggcttg gacggggcgt accgcctgcc gttaaccgtc gccgctgaaa
     7921 gggggatgta tggccataaa aggtcttgag caggccgttg aaaacctcag ccgtatcagc
     7981 aaaacggcgg tgcctggtgc cgccgcaatg gccattaacc gcgttgcttc atccgcgata
     8041 tcgcagtcgg cgtcacaggt tgcccgtgag acaaaggtac gccggaaact ggtaaaggaa
     8101 agggccaggc tgaaaagggc cacggtcaaa aatccgcagg ccagaatcaa agttaaccgg
     8161 ggggatttgc ccgtaatcaa gctgggtaat gcgcgggttg tcctttcgcg ccgcaggcgt
     8221 cgtaaaaagg ggcagcgttc atccctgaaa ggtggcggca gcgtgcttgt ggtgggtaac
     8281 cgtcgtattc ccggcgcgtt tattcagcaa ctgaaaaatg gccggtggca tgtcatgcag
     8341 cgtgtggccg ggaaaaaccg ttaccccatt gatgtggtga aaatcccgat ggcggtgccg
     8401 ctgaccacgg cgtttaaaca aaatattgag cggatacggc gtgaacgtct tccgaaagag
     8461 ctgggctatg cgctgcagca tcaactgagg atggtaataa agcgatgaaa catactgaac
     8521 tccgtgcagc cgtactggat gcactggaga agcatgacac cggggcgacg ttttttgatg
     8581 gtcgccccgc tgtttttgat gaggcggatt ttccggcagt tgccgtttat ctcaccggcg
     8641 ctgaatacac gggcgaagag ctggacagcg atacctggca ggcggagctg catatcgaag
     8701 ttttcctgcc tgctcaggtg ccggattcag agctggatgc gtggatggag tcccggattt
     8761 atccggtgat gagcgatatc ccggcactgt cagatttgat caccagtatg gtggccagtg
     8821 gctatgacta ccggcgcgac gatgatgcgg gcctgtggag ttcagccgat ctgacttatg
     8881 tcattaccta tgaaatgtga ggacgctatg cctgtaccaa atcctacaat gccggtgaaa
     8941 ggtgccggga ccaccctgtg ggtttataag gggagcggtg acccttatgc gaatccgctt
     9001 tcagacgttg actggtcgcg tctggcaaaa gttaaagacc tgacgcccgg cgaactgacc
     9061 gctgagtcct atgacgacag ctatctcgat gatgaagatg cagactggac tgcgaccggg
     9121 caggggcaga aatctgccgg agataccagc ttcacgctgg cgtggatgcc cggagagcag
     9181 gggcagcagg cgctgctggc gtggtttaat gaaggcgata cccgtgccta taaaatccgc
     9241 ttcccgaacg gcacggtcga tgtgttccgt ggctgggtca gcagtatcgg taaggcggtg
     9301 acggcgaagg aagtgatcac ccgcacggtg aaagtcacca atgtgggacg tccgtcgatg
     9361 gcagaagatc gcagcacggt aacagcggca accggcatga ccgtgacgcc ttccagcacc
     9421 tcggtggtga aagggcagag caccacgctg accgtggcct tccagccgga gggcgtaacc
     9481 gacaagagct ttcgtgcggt gtctgcggat aaaacaaaag ccaccgtgtc ggtcagtggt
     9541 atgaccatca ccgtgaacgg cgttgctgca ggcaaggtca acattccggt tgtatccggt
     9601 aatggtgagt ttgctgcggt tgcagaaatt accgtcaccg ccagttaatc cggagagtca
     9661 gcgatgttcc tgaaaaccga atcatttgaa cataacggtg tgaccgtcac gctttctgaa
     9721 ctgtcagccc tgcagcgcat tgagcatctc gccctgatga aacggcaggc agaacaggcg
     9781 gagtcagaca gcaaccggaa gtttactgtg gaagacgcca tcagaaccgg cgcgtttctg
     9841 gtggcgatgt ccctgtggca taaccatccg cagaagacgc agatgccgtc catgaatgaa
     9901 gccgttaaac agattgagca ggaagtgctt accacctggc ccacggaggc aatttctcat
     9961 gctgaaaacg tggtgtaccg gctgtctggt atgtatgagt ttgtggtgaa taatgcccct
    10021 gaacagacag aggacgccgg gcccgcagag cctgtttctg cgggaaagtg ttcgacggtg
    10081 agctgagttt tgccctgaaa ctggcgcgtg agatggggcg acccgactgg cgtgccatgc
    10141 ttgccgggat gtcatccacg gagtatgccg actggcaccg cttttacagt acccattatt
    10201 ttcatgatgt tctgctggat atgcactttt ccgggctgac gtacaccgtg ctcagcctgt
    10261 ttttcagcga tccggatatg catccgctgg atttcagtct gctgaaccgg cgcgaggctg
    10321 acgaagagcc tgaagatgat gtgctgatgc agaaagcggc agggcttgcc ggaggtgtcc
    10381 gctttggccc ggacgggaat gaagttatcc ccgcttcccc ggatgtggcg gacatgacgg
    10441 aggatgacgt aatgctgatg acagtatcag aagggatcgc aggaggagtc cggtatggct
    10501 gaaccggtag gcgatctggt cgttgatttg agtctggatg cggccagatt tgacgagcag
    10561 atggccagag tcaggcgtca tttttccggt acggaaagtg atgcgaaaaa aacagcggca
    10621 gtcgttgaac agtcgctgag ccgacaggcg ctggctgcac agaaagcggg gatttccgtc
    10681 gggcagtata aagccgccat gcgtatgctg cctgcacagt tcaccgacgt ggccacgcag
    10741 cttgcaggcg ggcaaagtcc gtggctgatc ctgctgcaac aggggggcca cctcgctggc
    10801 ggtggcgacc ggtgcgctgg cgtatgcctg gtatcagggc aactcaaccc tgtccgattt
    10861 caacaaaacg ctggtccttt ccggcaatca ggcgggactg acggcagatc gtatgctggt
    10921 cctgtccaga gccgggcagg cggcagggct gacgtttaac cagaccagcg agtcactcag
    10981 cgcactggtt aaggcggggg taagcggtga ggctcagatt gcgtccatca gccagagtgt
    11041 ggcgcgtttc tcctctgcat ccggcgtgga ggtggacaag gtcgctgaag ccttcgggaa
    11101 gctgaccaca gacccgacgt cggggctgac ggcgatggca cgccagttcc ataacgtgac
    11161 gacggagcag attgcgtatg ttgctcagtt gcagcgttcc ggcgatgaag ccggggcatt
    11221 gcaggcggcg aacgaggccg caacgaaagg gtttgatgac cagacccgcc gcctgaaaga
    11281 gaacatgggc acgctggaga cctgggcaga caggactgcg cgggcattca aatccatgtg
    11341 ggatgcggtg ctggatatcg gtcgtcctga taccgcgcag gagatgctga ttaaggcaga
    11401 ggctgcgttt aagaaagcag acgacatctg gaatctgcgc aaggatgatt attttgttaa
    11461 cgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag gcccgtcttg cgcttgaagc
    11521 cgcccgaaag aaggctgagc agcagactca acaggacaaa aatgcgcagc agcagagcga
    11581 taccgaagcg tcacggctga aatataccga agaggcgcag aaggcttacg aacggctgca
    11641 gacgccgctg gagaaatata ccgcccgtca ggaagaactg aacaaggcac tgaaagacgg
    11701 gaaaatcctg caggcggatt acaacacgct gatggcggcg gcgaaaaagg attatgaagc
    11761 gacgctgaaa aagccgaaac agtccggcgt gaaggtgtct gcgggcgatc gtcaggaaga
    11821 cagtgctcat gctgccctgc tgacgcttca ggcagaactc cggacgctgg agaagcatgc
    11881 cggagcaaat gagaaaatca gccagcagcg ccgggatttg tggaaggcgg agagtcagtt
    11941 cgcggtactg gaggaggcgg cgcaacgtcg ccagctgtct gcacaggaga aatccctgct
    12001 ggcgcataaa gatgagacgc tggagtacaa acgccagctg gctgcacttg gcgacaaggt
    12061 tacgtatcag gagcgcctga acgcgctggc gcagcaggcg gataaattcg cacagcagca
    12121 acgggcaaaa cgggccgcca ttgatgcgaa aagccggggg ctgactgacc ggcaggcaga
    12181 acgggaagcc acggaacagc gcctgaagga acagtatggc gataatccgc tggcgctgaa
    12241 taacgtcatg tcagagcaga aaaagacctg ggcggctgaa gaccagcttc gcgggaactg
    12301 gatggcaggc ctgaagtccg gctggagtga gtgggaagag agcgccacgg acagtatgtc
    12361 gcaggtaaaa agtgcagcca cgcagacctt tgatggtatt gcacagaata tggcggcgat
    12421 gctgaccggc agtgagcaga actggcgcag cttcacccgt tccgtgctgt ccatgatgac
    12481 agaaattctg cttaagcagg caatggtggg attgtcggga gtatcggcag cgccattggc
    12541 ggggctgttg gtggcggcgc atccgcgtca ggcggtacag ccattcaggc cgctgcggcg
    12601 aaattccatt ttgcaaccgg aggatttacg ggaaccggcg gcaaatatga gccagcgggg
    12661 attgttcacc gtggtgagtt tgtcttcacg aaggaggcaa ccagccggat tggcgtgggg
    12721 aatctttacc ggctgatgcg cggctatgcc accggcggtt atgtcggtac accgggcagc
    12781 atggcagaca gccggtcgca ggcgtccggg a
//
LOCUS       DAZFXT010000126        12680 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.126, whole genome shotgun sequence.
ACCESSION   DAZFXT010000126 DAZFXT010000000
VERSION     DAZFXT010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.126"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(50..379)
                     /locus_tag="ACHKW0_003914"
     CDS             complement(50..379)
                     /locus_tag="ACHKW0_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7476666.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWKPPYAYRQIKVTCAGWSARVGMLRVEFSA
                     EFKQVVN"
     gene            complement(376..3021)
                     /locus_tag="ACHKW0_003915"
     CDS             complement(376..3021)
                     /locus_tag="ACHKW0_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7476667.1"
                     /translation="MAGNFADLTAVLTLDSARFSEEAARVKKELGETSALADLMSGKV
                     SQSFRKQADAAEQSLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIVTQLAGGQNPFL
                     IMLQQGGQISDSFGGPLSLLTLLKEELLGIRDASESSEESLSDTANALAENARNAGEL
                     GRFMSVARVAAGGGVAVLAALAAAAWQAEQADRALLRSLTLTGGAAATTTAELWKMAG
                     VISDEAGGGIRQAAENLARLAESGKYTAGQLRIMGETSQRWLQTVGDDAGKVEKAFEG
                     IAADPVKALASLNQQYNFLSVSQLRHIDELERTKGKQAAVTEAMSLFADVMNARLEQL
                     DKAATPVEKIWDDVKTWTSDAWAWIGDHTLGALSLITDVVAGTVEQVKLLLVQGDLAL
                     AEFIQSAWETTKNVPGVGALFGELAEENRVFIEKTKRDELALRKSIAERDARIRQGEM
                     GYINRSRATGVSKGPGQQEAVSRLAEELTGKKHTSPKTRSAGEREEEQAREALLALEA
                     ELRTLEKHSGANEKISRQRRDLWKAESQYAVLKEAATKRQLSGQEKSLLAHEKETLEY
                     KRQLADLGDKVEHQKRLNELAQQAVRFEEQQSAKQAAISAKARGLTDRQAQRESEAQR
                     LRDVYGDNPQALARVTGALKQTWADEDMLRGDWLAGLKSGWGEWAESATDSFSQVKSA
                     ATQTFDGIAQNMAAMLTGAEADWRGFTRSVLSMMTEILLKQAMVGIVGRIGSAIGGAF
                     GGGASASSGTAIEAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGN
                     LYRLMRGYAEGGYVGGAGSPAQMRRAEGINFNQNNHVVIQNDGINGQAGPQLMKAVYD
                     MARKGAQDELRLQLRDGGMLSGSGR"
     gene            complement(3071..3373)
                     /locus_tag="ACHKW0_003916"
                     /pseudo
     CDS             complement(3071..3373)
                     /locus_tag="ACHKW0_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
     gene            complement(3400..3822)
                     /locus_tag="ACHKW0_003917"
     CDS             complement(3400..3822)
                     /locus_tag="ACHKW0_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7476668.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRFDYMKFVSDAAQQETTKHN
                     AVHINQRYLETASLLVAMSLWHSHSLKGTLASPETEMQQIRREVMLGWPADALNQATN
                     RVLYLSGMLDNRHDADPEPTGKAEATEPVTSKKHSKVS"
     gene            complement(3836..4588)
                     /locus_tag="ACHKW0_003918"
     CDS             complement(3836..4588)
                     /locus_tag="ACHKW0_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7476669.1"
                     /translation="MATPNPLEPVKGAGTTLWVYNGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRAVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(4596..4994)
                     /gene="gpU"
                     /locus_tag="ACHKW0_003919"
     CDS             complement(4596..4994)
                     /gene="gpU"
                     /locus_tag="ACHKW0_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail terminator protein"
                     /protein_id="HFO7476670.1"
                     /translation="MKHREIRAAVLSALKENISERVSWFDGRPVFIDEQELPAVAVYL
                     TDASAADEFVDEGTWEATLHIEVFLRAKEPDSALDMWMEEKILPALEAVPGLSALLLK
                     MNLQGYDYRRDDEFMMWGSADLLWKITYEM"
     gene            complement(5007..5630)
                     /locus_tag="ACHKW0_003920"
     CDS             complement(5007..5630)
                     /locus_tag="ACHKW0_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7476671.1"
                     /translation="MAIKGLAQAMKNLDAIDRRAVPRASATTLNRVAGAIIAKTASSV
                     ARELAVPRRLIRARIRLSPARPDKVYAKVYINTGNLPAIKLGEARVRLSRRKRRKKGQ
                     RAALKGGGSVLIVGKRRIPDAFITRLANGRWHVMQRMPWASSSTGADSKGRPKRHRLP
                     IEVVKITTAGPLAETFERERDRMYREKLPAQMMKAMTHQLRLVLKRK"
     gene            complement(5633..5914)
                     /locus_tag="ACHKW0_003921"
     CDS             complement(5633..5914)
                     /locus_tag="ACHKW0_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HFO7476672.1"
                     /translation="MSDLFTRMCCRMDVATVRVMGKQAEINGVVYDVMPEEESAEMGA
                     LSGSQLSLVVFSARYRPARHDVVVFAGRTLTVTRYDTYNGKPRIFVEQE"
     gene            complement(5907..6233)
                     /locus_tag="ACHKW0_003922"
     CDS             complement(5907..6233)
                     /locus_tag="ACHKW0_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsid cement protein"
                     /protein_id="HFO7476673.1"
                     /translation="MAKNFVQDGTTIELVNAGDQTILSGAAVVVGSMVAVAITDIPAG
                     EAGDGFAEGVFLLPKQSADDIQSGAVVYLKDGVVQLAADGAVAAGVAWENAPANSATV
                     AVKINV"
     gene            complement(6321..8300)
                     /locus_tag="ACHKW0_003923"
     CDS             complement(6321..8300)
                     /locus_tag="ACHKW0_003923"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpP-like prohead protease/major capsid protein
                     fusion protein"
                     /protein_id="HFO7476674.1"
                     /translation="MKNKSWFRMLAGSQGEADIYIYDEIGFWGVTAKQFVSDMNALGD
                     ITHINLHINSPGGDVFEGIAIFNALKNQGATITVYVDGVAASMASVIAMAGDTVIMPE
                     NAFMMIHKPWGFSGGDAEDMRSYADLLDKVESVLLPAYAQKTGKTTDEIAAMLADETW
                     MSGAECLAHGFADQVTPAVEAMACIQSKRTEEFKKMPESIRNMITPPRNSAPRDTTVT
                     IPAPAVTEPSPVPAVSDEATIRARVMAEQKARMSGINDLFAMFGGRYQTLQAQCVADP
                     DCSLEMARERLLNEMGKESSPTNKNTPAHIYAGNGNFVGDGIRQAMLARAGFENVEKD
                     NAYNGMTLREWARMSLTERGIGVASYNPMQMVGLALTHSTSDFGNILLDVSNKGLIQG
                     WEESEETFQKWTRKGRLSDFKTAYRVGMGGFGSLRQVREGAEYKYITTSDRKETIALA
                     TYGEIFSITRQAIINDDLNMLVDVPMKMGRAAKATIGDLVYKVLTDNPKLSDGKALFH
                     ADHKNIATGGISVSGLDAARQMMRLQKEGDRALNIRPAFMLVPVALETVANQTIKSAS
                     VKGADANAGVINPIQNFAEVIAEARLDAADPKTWYLAAAQGTDTIEVAWLDGVDTPYI
                     DQQEGFTTDGIATKIRIDAGVAPLDWRGLVRSSVA"
     gene            complement(8290..9792)
                     /locus_tag="ACHKW0_003924"
     CDS             complement(8290..9792)
                     /locus_tag="ACHKW0_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7476675.1"
                     /translation="MAIIDDVIGVFSPGWKAARLRSRALIMAYEAVKPTRTHKARREN
                     RSADQLSKYGAVSLREQARFLDINHDLVIGVFDKLEERVIGARGIIVEPQPLRKNGEM
                     AAELAADIRRLWAEWSVSPDVTGQYTRPVLERLLLRTWLRDGEVFAQMVSGAGNGLER
                     TAGVPFWLEAMEPDFVPMRTDESAGLNQGVFLDEWGRPKKYLVYKNYPVSGRQSDTKE
                     IAAGKMIHLKFTRRLHQTRGSSMLSGVLMRISALKEYEDAELTAARIAAALGLYIRKG
                     DGQDYEDPGSKETEREVHITPGIIYDDLRKGEDIGMVKSDRPNPNLETFRNGQLRAVA
                     AGSRLSFSSAARNYNGTYSAQRQELVESTDGYLILQDCFIGAVTRPVYRTWLNMVVAA
                     GLLKIPADVEMKTLYNATYSGPVMPWIDPVKEAEAWRIQIRGGAATESDWVRAGGRNP
                     DEVKRRRKAEIDENSRLGLVFDTDPVNDKGGNSAGTEQQRQQATDSQHEE"
     gene            complement(9792..10004)
                     /locus_tag="ACHKW0_003925"
     CDS             complement(9792..10004)
                     /locus_tag="ACHKW0_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476676.1"
                     /translation="MSTEEAREMIQRYREAEMAVLEGKSVTFNGQQLTLESLSQIRAG
                     RQEWERRLAAMVSRRRGKPGFKLARF"
     gene            complement(10001..12124)
                     /locus_tag="ACHKW0_003926"
     CDS             complement(10001..12124)
                     /locus_tag="ACHKW0_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7476677.1"
                     /translation="MNQVNESHSRASDIWREVASLFRPPGRLPVAEAIRRYMRVPRGA
                     NTSGPWESSLTPYMIDPINTLSAREYDAVVFVGPARTGKTEGLIDGWIVYGIICDPAD
                     MLVVQMTETKAREHSRTRLSRTFRHSPEVSKRLSPSRNDNNVHDKMFLDGSFLKIGWP
                     SITVFSSSDYRRVALTDYDRFPENVDGEGDAFTLASKRTTTFMSSGMTLVESSPGRDI
                     TDTKWRCGGAHEAPPTTGILSLYNRGDRRRWYWPCPHCGEYFQPVMDNMTGYRNNPDF
                     VAAGQAARLMCPHCRGLIAPEQKRELNNQGIWLREGERAAADGSITGTPRNSRIASFW
                     MEGPAAAFQTWEQLIFKLLAAEEEYERTGSEETLKAVVNTDIGRPYLPRSATEQRKSE
                     LLEQRAEPFPRRSVPDGVRFIEATVDVQGGKNRRFVVQITGYGEQGERWIVDRYNIRH
                     SLRCSPNGESLPVDPAAYPEDWDLLLTDVFHKTWPLASDPDVRMRLMAMAVDTGGEAG
                     VTDNAYRFWRRCRSDGLGNRVFLFKGDGLRRDRLINRTFPDNTGRSARRARASGDVAL
                     WLVQTDAFKDRVNNALWRDTPGPNYIHFPDWLGRWFYDELTYEERGSDGKWRKPGRGA
                     NEAFDLLVYADALAVLHGYEKIRWPSAPDWAQRETWLVFPQERSGETVSPELTAGAEK
                     RRRRKKKLRTERAEDNPWITSGGWL"
     gene            complement(12121..12597)
                     /locus_tag="ACHKW0_003927"
     CDS             complement(12121..12597)
                     /locus_tag="ACHKW0_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HFO7476678.1"
                     /translation="MDRELKNLHLNISQLAALSGAHRQTVAARVKNISPAGGHESNLK
                     LYRLTDILAELMKAPLPVDNEEMDPHARKAWYQSERDRLKFEQETGQLVPVSDVRRSF
                     SVVVKAIVQVLETWPDRLERDRGWTASQLNEVQIVVDEIRDTLEKAVIDCCDEADM"
BASE COUNT         2646 a         4066 c         2989 g         2979 t
ORIGIN      
        1 tgctctgatt taaccgactc gttcagactt tcttcatgaa tgtcctgcat cagttcacca
       61 cctgcttaaa ctccgcgctg aactcaacgc gcaacatccc gacccgcgca gaccacccgg
      121 cacaggtcac ctttatctgc cggtatgcat agggtggctt ccacaaaaat gccttccagc
      181 ccccgtgctc tgccagaaac gcttccagat gccgggcctc ctcccgggtc acggaaagcg
      241 tcacccggta tgttttcagg tcagcattca gccctgccgc catacgctgt gagtacccgt
      301 caccaaaacg cacttcacgc accgatggct gcgagttcac ctccatatcc ggcttcactt
      361 tccagcgaaa ggttttcatc gcccgctccc tgataacata ccgccatcac gcaactgcag
      421 ccggagttca tcctgcgccc ccttgcgggc catgtcatac accgccttca tcagctgcgg
      481 ccccgcctgt ccgttgatac cgtcgttctg aatcaccacg tgattgttct gattaaaatt
      541 aataccttcc gcccgccgca tctgcgccgg acttccggca ccacccacat aaccaccttc
      601 cgcatagccg cgcatcagac ggtaaagatt ccccacacct atccggctgg ttgcctcttt
      661 cgtgaaaaca aactccccgc ggtgaactat ccccgcaggc tcatatttgc cgcccgtccc
      721 cgtaaatcct ccggtcgcga aatggaagtt cgccgccgca gcctcaatgg ccgtccccga
      781 ggaagcagat gcaccaccac cgaaagcacc gccaatggcg ctgccgatac gcccgacaat
      841 gcccaccatg gcctgtttaa gcaggatttc tgtcatcatg gacagcaccg aacgggtgaa
      901 tccccgccag tctgcctctg caccggtcag catcgccgcc atattctgtg caataccgtc
      961 aaaggtctgc gtggcagcac ttttaacctg cgaaaaactg tccgtcgcac tttccgccca
     1021 ctccccccag ccggacttca gcccggccag ccagtcaccg cgcagcatgt cttcatccgc
     1081 ccatgtctgt ttcagtgccc cggtgacccg ggccagcgcc tgcggattat caccgtacac
     1141 gtcccgaaga cgctgcgctt cagactcccg ctgcgcctga cggtcagtga gaccgcgggc
     1201 ttttgcgctg atggcggcct gcttcgcgct ctgctgctct tcaaaccgca ccgcctgctg
     1261 tgccagctca ttcaggcgtt tctggtgttc aaccttgtcg cccaggtcag ccagctggcg
     1321 tttgtactcc agcgtctctt tctcatgggc cagcagggat ttttcctgcc cggataactg
     1381 ccgtttcgtg gcagcctctt tcaggaccgc atactgactt tccgccttcc ataaatcacg
     1441 gcgctgccgg ctaattttct cattcgcacc gctgtgtttt tccagcgtcc tgagctcagc
     1501 ttcaagggca agcagagcct ctcttgcctg ctcctcttcc ctctccccgg cagagcgcgt
     1561 tttcggtgat gtatgctttt tacctgtcag ctcttcagcc agacggctga cggcttcctg
     1621 ctgcccagga cctttgctga cgcctgttgc acgcgagcgg ttgatgtacc ccatttcccc
     1681 ctggcgtata cgcgcatccc gttccgcaat ggattttctc agcgccagtt catcgcgttt
     1741 tgttttctca ataaatacgc ggttctcttc tgccagttca ccaaacaacg caccaacgcc
     1801 gggcacattc tttgtcgttt cccaggctga ctgaataaat tcagccagcg ccagatcccc
     1861 ctgcacaagc agcagcttca cctgttcaac ggttccggcc accacgtcag tgatcagact
     1921 gagtgccccc agtgtatgat cacctatcca tgcccatgcg tcagaagtcc aggttttaac
     1981 atcgtcccag attttttcca ccggcgtggc cgctttatca agttgctcca gacgtgcatt
     2041 catgacatcc gcaaacaggg acatcgcctc cgtcaccgca gcctgtttac ctttcgtgcg
     2101 ctcaagctca tcaatatggc gtaactggga aacgctcagg aagttatact gctgattcag
     2161 ggaggccagc gccttcaccg gatctgctgc aatcccttca aaggcttttt ccaccttccc
     2221 ggcatcgtcc cccaccgtct gcagccatct ctgagaggtt tcccccatga tccgtagctg
     2281 cccggcggta tatttcccgc tttctgccag acgggccaga ttttctgccg cctgtctgat
     2341 accaccaccg gcttcatcgc tgatcacccc ggccattttc cacaattctg ccgttgtggt
     2401 ggcagcagcc cctccggtca gggtcagtga acgcaataag gcccggtcag cctgctctgc
     2461 ctgccaggcg gcggcagcaa gcgcggccag tacggcaacc ccgccacctg ccgccacacg
     2521 ggccaccgac ataaatcgtc ccagctcacc ggcattccgg gcattttcag ccagtgcatt
     2581 tgccgtatct gacagcgact cctctgatga ttcagaggca tccctgatcc cgagaagttc
     2641 ctccttcagc agggtaagca ggctgagcgg tccaccgaat gaatcgctga tctgcccccc
     2701 ctgctgcagc atgataagga agggattctg accaccggca agctgagtga caatatccgt
     2761 gaactgtgcg ggcagtgtgc gcatggcagc cttatactgt ccgactgata tcccggcttt
     2821 ttgtgcagcc agcgcctgtc ggctcagact ctgctcagca gcatcagcct gttttctgaa
     2881 agactgactg actttcccgg acatcaaatc agcaagcgca ctggtttcac ccagctcttt
     2941 ttttacccgc gctgcctctt cagaaaaacg ggcagaatcc agtgtcagca cggctgtcag
     3001 atcggcaaaa ttacctgcca tcgtggtcac ctcctgaaat gtcctctgat accatcaata
     3061 actgtcacaa cctccttccc tcctccccga aacggactcc accggcgagg cccgccgcct
     3121 tctgcatcag tacatcattt tcgtccggcg tctccgcctg tccttcctct gcctccggag
     3181 cgaacaggct gaaatccgcc ggatgcatat ccggatcgca aaaaaacagg ctgagtacgg
     3241 cgtacgtcag cccggaaaaa tgcatatcca gctgggtatc ctgaaaataa tgcgtgcagt
     3301 aaaaacgtcg ccagtcggca tattcggtgg atgtcatccc ggcaagcatg gcgcgccagt
     3361 cggctctccc catctcacgc gccagtttca ggacaaagtt cagctcacct tcgaatgctt
     3421 ttttgatgtt accggctctg tcgcttctgc tttcccggtt ggttcaggat cggcatcgtg
     3481 ccggttatcc agcatacctg aaagataaag cacccggttc gttgcctgat tcagtgcatc
     3541 agcaggccat cccagcatca cttcacggcg gatctgctgc atctctgtct ccggagaggc
     3601 cagagtgcct ttgagggaat gggaatgcca tagcgacatc gccacaagca gggatgccgt
     3661 ttccagatat cgctggttaa tgtgcacggc attatgcttc gttgtctcct gttgtgctgc
     3721 gtctgaaaca aacttcatat aatcaaaccg ctgcagcgca gacagctccg acagcgtgac
     3781 ggacacaccg ttatattcaa attgttctgt tttcagaaac atgtattacc tccgtttacc
     3841 ctgcagcgcc cgcttcagta acggtgactt cagccactgc ggcgaactga ccatttccgc
     3901 tcaccacagg gatctgcacc ttacctgtcg ccacgccgtt taccgtaatt gtcatatctt
     3961 tcacactaat ggtggctttc gacggatcgg cggaaaccgc tctgaacgtc ttgtcggttg
     4021 cactttccgg ctcaaaagaa accgtcaggg tggttgtttt cccttttgcc accgtaccgg
     4081 atgtcggtgt caccttaatt gcagtggccg gcgtaatttc gctgcgttct tccgccacgg
     4141 aaggtttgcc cacgttagtg actttcaccg tgcgggtgat cacttctttc gccgtcacgg
     4201 ccttaccgat actgctgacc cagccacgga acacatccac cgtgccattt gggaaacgga
     4261 ttttataggc ccgcacatcc ccgctttcaa accagcctat aagccctttc tgaccttctt
     4321 ctcccggttt ccaggccagc gtaaaactgg tatctcctgc agacttctgc ccctgcccgg
     4381 tcgcggtcca gtccgcgtct tcatcatcca ggtagttatc atcgtagggt tctgccgtca
     4441 tctcgcccgg cgtcagatcc ttcaccttag ccagtcgctg ccagtcatcg tctgacaacg
     4501 ggtttgcata agcatcaccc ttgccgttgt aaacccacag agtggtaccg gcacctttta
     4561 ccggctcaag gggatttggt gttgccatat cgtcctcaca tctcgtaggt aattttccac
     4621 aggagatctg ccgatcccca catcataaac tcatcatccc ggcggtagtc atacccctga
     4681 agattcatct tcagcagtaa tgcactgagc ccgggaactg cctccagcgc aggaagaatt
     4741 ttttcttcca tccacatatc cagtgccgag tccggttctt ttgccctgag aaaaacctca
     4801 atatgcagtg tcgcctccca ggttccctca tcaacgaact cgtcagcagc agacgcatct
     4861 gtcaggtaaa cagcaacagc aggcagttcc tgttcatcaa taaaaaccgg gcgaccgtca
     4921 aaccagctca ccctctcaga aatattttct ttcagggcag acagaactgc cgcccgtatt
     4981 tcacggtgtt tcatacaccc tcccagtcat tttcttttca gcaccaggcg taactgatgc
     5041 gtcatggctt tcatcatctg cgccggtaat ttttcccggt acatccggtc ccgttcacgt
     5101 tcaaaggttt ctgccagcgg tccggcagtc gtaatcttca ccacttcgat cggcagacgg
     5161 tggcgtttcg gcctcccttt gctgtccgcg ccggtggatg atgatgccca cggcatacgc
     5221 tgcatcacat gccagcgtcc gttagccagc cgggtgataa aggcgtccgg gatccgtctt
     5281 ttccccacaa tcagcacact gccgccccct ttcagggccg cacgctgtcc tttctttctc
     5341 cgttttctgc gggaaagtcg aacgcgggcc tcccccagtt tgatggcggg caggttgccg
     5401 gtattgatgt aaacctttgc gtaaacctta tccggtcgtg ccggacttaa ccggatgcgg
     5461 gcacggataa gacgacgggg aacggccagc tccctggcaa ctgaagaggc cgttttcgca
     5521 ataatggccc ccgccactcg gttcagtgtc gtggcagagg cccggggaac ggcacggcga
     5581 tcaattgcat ccagattttt catggcctgc gccagacctt ttattgccat actcattcct
     5641 gttcgacaaa aatccggggt ttaccgttgt acgtgtcata acgggtcacc gtcagtgtgc
     5701 gccccgcaaa cacaacaaca tcatgacggg ccggacggta ccgggctgaa aacaccacca
     5761 gtgacaactg gctgcccgaa agcgccccca tctccgcgga ctcttcctcc ggcatcacgt
     5821 cgtacacgac gccgttaatc tccgcctgtt tgcccatcac ccgaacggtc gccacgtcca
     5881 tccggcaaca cattcgcgta aacagatcag acattgattt ttaccgccac agtggcgctg
     5941 tttgcagggg cattttccca ggctaccccc gctgccaccg caccgtctgc agccagctgc
     6001 acaaccccgt ccttcagata aaccaccgcg ccggactgaa tgtcgtcagc agactgtttg
     6061 ggcagcagga acacgccttc ggcaaaaccg tcaccggcct caccggcagg aatatcggta
     6121 atggccacgg ccaccatact gccgaccacc accgcagcac cgctcaggat ggtctgatct
     6181 ccggcattca ccagttcaat ggtggtaccg tcctgtacaa aatttttcgc cataatgctg
     6241 tttctccgga cagcccctgt ggggctgttt ttcaggcata aaaaaagccc tttcgggcag
     6301 tgattgtgat aacgcggtta tcaggccacc gacgaacgca ccagcccgcg ccagtcaagt
     6361 ggtgccactc cggcatcaat acggattttt gtggcaatgc cgtcagtggt gaaaccttcc
     6421 tgctgatcaa tgtatggcgt gtccacacca tccagccagg ccacttcaat ggtgtcagtg
     6481 ccctgtgccg ccgccagata ccaggttttc gggtctgccg catcaagacg cgcttctgca
     6541 atcacctcag caaagttctg gatagggtta atgacaccgg cgtttgcatc cgcccctttc
     6601 acactggccg atttgatggt ctggttcgcc accgtctcca gtgccaccgg taccagcata
     6661 aaggccggac ggatattcag ggcacgatcg ccttctttct gcaggcgcat catctgacgg
     6721 gccgcatcca gtccggaaac ggagatcccc ccggtggcaa tatttttgtg atcggcatgg
     6781 aacagcgcct taccgtcgga cagtttcggg ttatccgtca gcaccttgta gaccaggtca
     6841 ccaatcgttg ccttcgccgc acgccccatc ttcatcggca cgtccaccag catattcaga
     6901 tcatcattga taatggcctg gcgggtgatg gagaaaatct ccccgtaagt ggccagtgca
     6961 atggtctcct tgcgatctga ggtggtgatg tatttatact ccgccccctc acgaacctgg
     7021 cgcagagaac caaaaccgcc catccccacg cgatacgctg ttttgaagtc tgacaggcgt
     7081 cccttacggg tccacttctg gaaggtttct tctgattcct cccagccctg gatcagcccc
     7141 ttgttcgaca catccagcag aatattgcca aaatcagagg tgctgtgcgt cagcgccagc
     7201 ccgaccatct gcatggggtt ataactggcc accccaatac cgcgctccgt cagtgacatg
     7261 cgagcccatt cacgcagggt catcccgtta taggcgttat ccttctcgac attttcaaat
     7321 ccggcacggg ccagcatcgc ctggcggatc ccgtccccca caaaattgcc gtttccggca
     7381 taaatatggg ccggtgtatt tttgttggtc ggcgaggact ccttgcccat ttcattcagc
     7441 agtcgttcac gggccatttc cagcgaacag tcaggatcag ccacgcactg tgcctgaagc
     7501 gtctgatagc gaccaccgaa catggcaaac agatcgttaa tgcctgacat gcgggctttc
     7561 tgttctgcca taacgcgggc gcgaatggtc gcctcatcag acactgccgg taccggtgat
     7621 ggttctgtta ccgccggtgc agggattgtc actgtggtat cacgcggggc actgttgcgt
     7681 ggcggagtaa tcatgtttcg gatggattcc ggcatctttt taaattcctc tgtacgtttt
     7741 gactgaatac atgccattgc ctcaacagcg ggtgtcacct ggtcagcaaa tccgtgtgcc
     7801 agacattcgg caccggacat ccaggtttca tccgccagca tggcggcaat ttcatcggtg
     7861 gtttttccgg ttttctgcgc ataggctggc aacagtaccg attcgacttt atccagcaaa
     7921 tcggcataac tgcgcatatc ctcagcatcc ccgccactga atccccatgg cttatggatc
     7981 atcatgaagg cattttccgg cataatgacc gtatcaccgg ccatcgcaat cacagatgcc
     8041 atcgaggcgg caacgccatc cacatacacg gtaatggtcg ccccctgatt tttcagggca
     8101 ttaaaaatgg cgatgccttc aaagacatcg ccacccggtg aattgatatg gagattaatg
     8161 tgggtgatat cacccagggc attcatatcg ctgacaaact gcttcgcggt aactccccag
     8221 aaaccaatct cgtcataaat ataaatatct gcctcaccct gactacccgc cagcatcctg
     8281 aaccaggatt tattcttcat gctggctgtc ggtggcctgc tgacgctgtt gttcagttcc
     8341 ggcactgttg cctcctttgt cgttgacggg gtcagtatca aagaccagcc ccagtctgct
     8401 gttttcatca atttcagcct tgcggcgacg tttgacctca tccggattgc gcccaccagc
     8461 acgcacccag tcagattctg tcgctgcacc accccggatc tgaattctcc aggcttcagc
     8521 ttccttaacc gggtcgatcc acggcatcac cggaccggaa tacgtcgcgt tatatagcgt
     8581 tttcatctcc acatccgccg gaattttcag cagacctgcc gcaaccacca tattcagcca
     8641 tgtccggtac accgggcggg ttaccgcgcc aataaaacag tcctgcagga tcaggtaacc
     8701 atccgtggac tcgaccagct cctgccgctg ggcgctgtag gtgccgttat agttacgcgc
     8761 cgcactggaa aaactcagac gactgcctgc tgccactgca cgcaactggc cgttgcggaa
     8821 agtttcaagg ttgggattgg gacggtcaga tttgaccatg ccgatatcct cgcccttgcg
     8881 caaatcgtca taaataatac ccggggtgat atggacttcc cgctcggtct ctttgctccc
     8941 cggatcttca tagtcctgtc cgtcaccttt acggatatac agtcccagcg ccgcagcaat
     9001 acgcgccgct gtcagttccg catcctcata ctccttaagg gcactgatcc gcatcagcac
     9061 ccccgataac atggatgagc ctcgcgtctg atgcagacga cgagtgaact tcaggtggat
     9121 catttttccg gcagcgattt ctttcgtatc actctgccgg ccgctgaccg gataattttt
     9181 ataaaccaga tattttttcg gtcttcccca ctcatcaaga aaaaccccct gattcagtcc
     9241 ggcggattca tcagtgcgca tgggaacaaa atccggctcc atcgcctcaa gccagaatgg
     9301 cactcccgcc gtccgttcca gaccgtttcc cgcaccactg accatctgcg caaacacttc
     9361 accatcccgc agccaggtcc gcagcagtaa acgttcaagc acaggacggg tatactgccc
     9421 tgtcacatcc ggactcacgg accattcagc ccacaaacgg cggatatccg cagccagctc
     9481 agccgccatt tccccgtttt ttcgtaatgg ctgaggctcc acaataattc ccctggcacc
     9541 aatcacccgc tcttccagct tgtcaaacac accaatcacc aggtcatgat tgatatccag
     9601 aaaacgggcc tgctcccgca gggaaaccgc accgtattta ctgagctgat cagcagagcg
     9661 attttcccgc cgggctttat gtgtccgggt cggtttcacc gcctcatagg ccatgattaa
     9721 cgcccttgaa cgcagtctgg ctgctttcca cccgggggaa aacacgccga tcacatcatc
     9781 aataattgcc attaaaacct cgccagttta aatcccggtt ttccccgcct gcggctcacc
     9841 atcgcggcaa gcctgcgttc ccactcctga cgtccggcgc ggatctgaga aaggctttcc
     9901 agcgtcagtt gctgcccgtt gaaggtgaca gactttccct ccagtacggc catttccgct
     9961 tcacggtacc gctgtatcat ttctctggct tcttctgtgc tcacaaccag cctcctgatg
    10021 ttatccatgg attatcttcc gcacgctccg tccgcagttt tttcttccgg cgacggcgtt
    10081 tttctgcccc ggccgtcagt tccggggata ccgtttcacc agaacgctcc tgcgggaaga
    10141 cgagccacgt ttcccgctgt gcccagtccg gtgcggaggg ccagcggatc ttttcgtaac
    10201 catgcagaac ggcaagcgca tccgcataaa ccagcaggtc aaacgcttcg ttagcgcccc
    10261 tgcccggttt tcgccatttt ccgtcactgc cgcgctcttc ataggtcagc tcatcgtaaa
    10321 accaccgccc cagccagtcg ggaaagtgga tatagttcgg ccctggtgtg tcacgccaca
    10381 gggcattatt tacacgatcc ttaaacgcat ccgtctgaac cagccacagc gcgacatcgc
    10441 cactggctct ggcacggcgg gcacttctgc cggtattatc cgggaaggtt cggttaatca
    10501 gcctgtcacg gcgaagtcca tcccccttga acagaaacac cctgttgccc agtccgtcac
    10561 tccggcaacg acgccagaaa cgataggcgt tatctgtcac cccggcttcc cctcccgtat
    10621 ccaccgccat ggccatcaga cgcatgcgca catccggatc agaagccagc ggccatgttt
    10681 tatggaacac atccgtcagc aacaaatccc agtcctccgg atatgccgcc ggatcaaccg
    10741 gcagactttc accgttggga ctgcagcgca gtgaatgccg gatgttgtag cgatcaacaa
    10801 tccagcgttc cccctgctct ccgtatccgg tgatctgcac aacaaaacgg cgatttttac
    10861 cgccctgtac gtcaaccgtt gcctcaataa aacgcacacc atccggcaca gatcgccggg
    10921 gaaacggctc ggcacgctgt tcaagcagtt cacttttacg ctgttccgtg gctgaacggg
    10981 gcagataggg tcgtccgata tcggtgttca ccaccgcttt cagggtctct tcactgccgg
    11041 ttcgctcata ctcttcttct gccgccagca gtttaaaaat cagttgttcc caggtctgaa
    11101 acgccgcagc tggcccctcc atccaaaatg acgcaatccg ggagtttcgt ggcgttccgg
    11161 tgatactgcc gtccgccgcc gcccgttcac cttcacgaag ccagatcccc tggttattca
    11221 gttcgcgttt ctgctcaggg gcaatcagcc cgcgacaatg cggacacatc agacgggcag
    11281 cctgaccggc agccacaaaa tccgggttat tccggtatcc ggtcatgtta tccatcaccg
    11341 gctgaaaata ttccccgcag tgcggacacg gccagtacca ccggcggcgg tctccccggt
    11401 tatacagtga caggataccc gttgttggcg gtgcctcatg tgcgccacca caacgccatt
    11461 tggtatcggt gatatcccgc cccggtgaac tctcgaccag ggtcatcccc gaggacataa
    11521 aggtggtggt acgctttgag gccagcgtga aggcatcccc ttccccgtcc acgttttcag
    11581 ggaaacggtc ataatccgtc agcgccacac gacggtaatc cgaagaggaa aagacggtga
    11641 tcgacggcca gccaatcttc aggaaggagc cgtcaagaaa cattttatcg tggacgttgt
    11701 tgtcattacg ggaaggactg aggcgcttgc tgacctccgg actgtggcga aacgtcctgg
    11761 aaagacgcgt tctggaatgc tcacgcgcct tcgtctcagt catctgcacc accagcatat
    11821 ccgccggatc acagatgatg ccgtacacaa tccagccatc aatcagccct tcggttttcc
    11881 cggttcgcgc aggtcccaca aacaccaccg cgtcatattc acgggctgat aatgtattaa
    11941 tggggtcaat catatagggc gtcagcgatg actcccacgg accggaagta ttggctcccc
    12001 gtggaacccg catataacgc ctgatggctt ccgctactgg taaccggcca ggtgggcgaa
    12061 acagcgaggc cacttcgcgc cagatatcgg atgcgcggct atggctctcg ttcacctgat
    12121 tcacatatcg gcctcatcac aacagtcaat gactgccttt tccagtgtgt cgcggatctc
    12181 atcaaccaca atctgtactt cattcagttg tgatgcggtc caccccctgt ccctctccag
    12241 ccggtcaggc caggtttcca gtacctgaac tatcgctttc accacgacag aaaaggaccg
    12301 cctgacatca ctgactggca caagctgacc agtttcctgc tcaaatttca gtcggtcacg
    12361 ttctgactgg taccatgctt tacgcgcatg aggatccatt tcctcgttat ctacaggcag
    12421 aggagctttc atcagctcgg caaggatatc tgtcagtcgg tacagtttga gattgctctc
    12481 atgaccaccg gctgggctta tgttttttac ccgagccgca acagtctgtc gatgagcacc
    12541 ggataatgcg gccagttggg aaatattcag atgcagattt tttaattcac gatccataac
    12601 tccccctgaa aattatgtaa acacacacca gtgatgaaca aaaaacaacc agattcgaca
    12661 ctaaaaattt ttatttttct
//
LOCUS       DAZFXT010000127        12648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.127, whole genome shotgun sequence.
ACCESSION   DAZFXT010000127 DAZFXT010000000
VERSION     DAZFXT010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.127"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1292
                     /locus_tag="ACHKW0_003928"
     CDS             <1..1292
                     /locus_tag="ACHKW0_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HFO7476679.1"
                     /translation="LPLVLFILALCFKVKPAKALRSSLTVGIGFVGIYAIFDILTSNV
                     GPAAQAMVERTGINLPVVDLGWPPLSAITWGSPIAPFVIPLTILINVAMLALNKTRTV
                     DVDMWNYWHFALAGTLVYYSTGSLFFGLLAAAIAAVVVLKLADWSAPLVQKYFGLEGI
                     SLPTLSSVVFFPVGLLVDKIIDHIPGLNRIHIDPETVQKKFGIFGEPMMVGTILGILL
                     GVIAGYDFKKVLLLGISIGGVMFILPRMVRILMEGLLPLSEAIKKYLNAKYPDRDDLY
                     IGLDIAVAVGNPAIISTALLLTPISVFIAFVLPGNEVLPLGDLANLAVMASMIALASR
                     GNIFRTVLAAIPVIIADLWIATKIAPFITGMAKDVNFKFAEGSSGQVSSFLDGGNPFR
                     FWLLEIFNGNIIAIGLVPVIALVLYGIFRITRSTVYA"
     gene            1285..2793
                     /locus_tag="ACHKW0_003929"
     CDS             1285..2793
                     /locus_tag="ACHKW0_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HFO7476680.1"
                     /translation="MPDNSAAIVIDIGTTNCKVTCFSCLDATTLGAHKFVTAKQISPQ
                     GDVDFDIDALWQEVRQAIAQLNAASPLPVRRISIASFGESGVFLDEHGEILTPMLAWY
                     DRRGEEYLATLSEADSAALYDICGLPLHSNYSAFKMRWLLEHYPLRNRRGLRWLHAPE
                     VLLWRLTGEQRTDITLASRTLCLDVRKGEWSAKAAALLHVPCTAFAPLVQPGEHAGWV
                     SESLCKTLGFSQPVSVTLAGHDHMVGARALQMMPGDILNSTGTTEGILQLDTQPTLDE
                     QAKRDKLANGCYSLANQFTLFASLPVGGFALEWLRNTFRLTDEEIAASLTRGHADYLA
                     GNWLLDDIPVFIPHLRGSGSPYKNRHTRGLFYGLGDTLSIDMLIASVSLGLTMEFANC
                     FACFNVPGTSALKVIGPATHNPLWLQLKADILQRPVEAIAFNEAVSVGALLTAAPDIP
                     PPPVAIAQRLLPNRARYHQLQRYQHKWKSWYQLKLQQEGVMPLHHREEHYVE"
     gene            2783..3052
                     /locus_tag="ACHKW0_003930"
     CDS             2783..3052
                     /locus_tag="ACHKW0_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HFO7476681.1"
                     /translation="MLNKTVEVRNSTGLHARPAACLAKAAKKYSCKVTLHYEGNDINA
                     TSMMNIMRAGIKGGKTVEIRCDGDDENEAIQTLTDLFRDRFGEAE"
     gene            3084..3944
                     /locus_tag="ACHKW0_003931"
     CDS             3084..3944
                     /locus_tag="ACHKW0_003931"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase"
                     /protein_id="HFO7476682.1"
                     /translation="MPLVNGRILLDRIQEKRVLAGAFNTTNLETTISILNAIERSGLP
                     NFIQIAPTNAQLSGYDYIYEIVKRHADKMDVPVSLHLDHGKTLEDVKQAVRAGFTSVM
                     IDGAAFSFEENIAFTQEAVDFCKSYGVPVEAELGAILGKEDDHVSEADCKTEPEKVKT
                     FVERTGCDMLAVSIGNVHGLDDIPRIDIPLLKRIAEVCPVPLVIHGGSGIAPEILRSF
                     VNYRVAKVNIASDLRKAFITAVGKAYVNNHNEANLARVMASAKNAVEEDVYSKILMMN
                     EGHRLVRKAS"
     gene            complement(3994..4353)
                     /locus_tag="ACHKW0_003932"
     CDS             complement(3994..4353)
                     /locus_tag="ACHKW0_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO7476683.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLLEYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(4350..4625)
                     /locus_tag="ACHKW0_003933"
     CDS             complement(4350..4625)
                     /locus_tag="ACHKW0_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO7476684.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            complement(4698..5822)
                     /gene="yhhJ"
                     /locus_tag="ACHKW0_003934"
     CDS             complement(4698..5822)
                     /gene="yhhJ"
                     /locus_tag="ACHKW0_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HFO7476685.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(5822..8557)
                     /gene="rbbA"
                     /locus_tag="ACHKW0_003935"
     CDS             complement(5822..8557)
                     /gene="rbbA"
                     /locus_tag="ACHKW0_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HFO7476686.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDILPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(8554..9621)
                     /gene="yhiI"
                     /locus_tag="ACHKW0_003936"
     CDS             complement(8554..9621)
                     /gene="yhiI"
                     /locus_tag="ACHKW0_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7476687.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHMRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(9684..9797)
                     /gene="yhiY"
                     /locus_tag="ACHKW0_003937"
     CDS             complement(9684..9797)
                     /gene="yhiY"
                     /locus_tag="ACHKW0_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhiY"
                     /protein_id="HFO7476688.1"
                     /translation="MMTTLLPVFTKPSPLALNALRAGRICRFLLIPDGRIR"
     gene            complement(9987..11609)
                     /gene="yhiJ"
                     /locus_tag="ACHKW0_003938"
     CDS             complement(9987..11609)
                     /gene="yhiJ"
                     /locus_tag="ACHKW0_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO7476689.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHILRR
                     DNNYQFIVTINSKNYGCYLDNTDINWCSIVYLLNNMTVNDDANDVAVTESYKPVWDWK
                     ISQFNVSDIKFETMINPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLRIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDPTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYAGIKDYPVCFYGPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(11871..12648)
                     /locus_tag="ACHKW0_003939"
                     /pseudo
     CDS             complement(11871..12648)
                     /locus_tag="ACHKW0_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
BASE COUNT         3117 a         3257 c         3037 g         3237 t
ORIGIN      
        1 tgttgccgtt ggtgttattt attcttgctc tctgttttaa agtaaaaccc gctaaagcct
       61 tacgttcgtc attaacagtc ggcattggtt ttgtcggtat ttatgccatt ttcgatattc
      121 tgaccagcaa tgtcgggcca gcggcccagg cgatggttga acgcaccgga attaatttac
      181 cggtggtaga tttaggctgg ccgccgcttt ccgctattac atggggttcg ccaattgccc
      241 cgtttgttat ccccctgacc attctgatta acgtggcgat gctggcgtta aataaaaccc
      301 gtaccgttga cgtagatatg tggaactact ggcattttgc ccttgctggt acgttggttt
      361 attacagcac cggcagcctg ttctttggtt tgctggcggc ggcgattgct gcggtggtag
      421 tacttaaact cgccgactgg tctgcgccac tggtacaaaa atactttgga ctggaaggga
      481 tctcattgcc gacgctctct tcggtggtgt tcttcccggt cggtctgctg gtcgacaaaa
      541 tcatcgacca tatccccggc ctcaatcgta ttcatatcga cccggaaacc gtacagaaaa
      601 agtttggcat cttcggcgaa ccgatgatgg ttggcactat tctgggcata ctgctcggcg
      661 tgattgcagg atacgatttc aaaaaagtgt tgctgcttgg catcagcatt ggcggtgtga
      721 tgttcatcct gccacgcatg gtacgcatcc tgatggaagg tttattaccg ctgtctgaag
      781 ccattaaaaa gtatctcaat gccaaatacc ctgaccgtga cgatctctat atcggcctgg
      841 atatcgccgt tgccgtaggt aacccggcga ttatctccac cgccctgctg ctgacgccta
      901 tctcggtctt tatcgcgttt gtccttccgg gtaatgaagt cctgccgctt ggcgaccttg
      961 ccaacctggc ggtaatggcg tcgatgattg ctttagccag ccgtggcaat attttccgca
     1021 ccgttctggc ggcgatcccg gtgattattg ccgacctgtg gattgcgact aaaatcgcac
     1081 cgtttattac cggcatggcg aaagacgtta acttcaaatt tgccgaaggc tccagcggcc
     1141 aggtttccag tttccttgat ggcggtaacc cgttccgctt ctggctgctg gaaatcttca
     1201 acggcaatat catcgccatt ggtctggtgc cggttatcgc cctggtactg tatggcattt
     1261 tccgaataac gcggagcacg gtttatgcct gataacagcg cagctatcgt tatcgatatt
     1321 ggcaccacca attgcaaagt cacctgcttt tcctgcctgg acgcaacgac gttgggcgcg
     1381 cataaattcg tgacggcaaa gcagatctcc ccacagggcg atgtcgattt cgatatcgac
     1441 gccctctggc aggaggtccg ccaggcgata gcgcaactga acgccgcttc gccgctgcca
     1501 gtcagacgga tcagcattgc cagttttggc gaatcaggcg tgttccttga cgagcatggc
     1561 gagatcctga cgccaatgct ggcatggtat gaccgtcgcg gtgaagagta tctggcaacg
     1621 cttagcgagg cagacagtgc agcactttat gacatctgcg gtctaccact acacagcaat
     1681 tactctgcct tcaaaatgcg ctggttgctg gaacattacc cgctgcgtaa tcgccgcggc
     1741 ctgcgctggc tacatgcgcc ggaagtgctg ctctggcggc tgactggcga acagcgcacg
     1801 gatatcacct tagccagccg cacgctgtgt ctggacgtgc gcaaaggcga atggtcagcg
     1861 aaagcggcgg cgttgttaca cgttccctgt acggcatttg cgccattggt gcagccaggc
     1921 gagcacgccg gatgggtcag cgagtcactt tgcaagacgc ttgggttctc gcaaccggtc
     1981 agcgtgacgc tggccggaca tgaccatatg gtgggtgcgc gagcgttgca gatgatgcca
     2041 ggcgatatcc ttaactcgac ggggaccacg gaaggcattc tgcaactgga tacacaaccg
     2101 acgctggatg aacaggccaa acgtgacaag ctggcaaacg gctgttactc acttgccaac
     2161 cagttcaccc tgtttgcgtc gctgcccgtg ggcggtttcg cgctggagtg gctgcgcaac
     2221 acgttccggc taaccgatga ggagatcgcc gcatcactta ctcgcggaca tgctgattat
     2281 ctggcgggga attggttgct cgatgacatt cccgtcttta ttccacatct tcgcggttcg
     2341 ggttcgccct ataaaaatcg ccatacccgt ggattatttt atgggcttgg cgatacgtta
     2401 agtatcgaca tgttaattgc cagcgtatca ctgggattaa ccatggaatt tgccaactgc
     2461 ttcgcctgtt ttaacgtgcc tggcaccagc gcgttaaaag tgatcggtcc ggcaacccat
     2521 aatcctcttt ggctgcaatt aaaggcggat attttacagc gtccggttga agcaattgca
     2581 tttaacgagg cggtttctgt cggagcatta ttaaccgccg caccggatat tccaccgccg
     2641 ccagtcgcta tagcccaacg tttgttaccg aatcgggcga gataccatca attacagcgt
     2701 tatcagcaca aatggaaaag ctggtatcag ttgaaattac aacaagaagg cgtgatgcca
     2761 ttacatcatc gggaggaaca ctatgttgaa taaaaccgtg gaggtcagaa acagtactgg
     2821 cctgcatgca cgtccggcgg cctgtctggc gaaagccgcc aaaaaatata gctgtaaagt
     2881 gacgctgcac tatgagggta atgatattaa tgccaccagc atgatgaata ttatgcgcgc
     2941 cggaataaaa ggcggaaaaa cggtggaaat acgctgcgac ggtgatgatg aaaacgaggc
     3001 catacagact ctcaccgact tatttcgcga ccgcttcggt gaagctgaat aattaactga
     3061 tattaaaaga tgaggttttc aatatgccat tagttaatgg acggatactg ctcgaccgca
     3121 ttcaggaaaa acgggtatta gcgggcgcat ttaataccac caatctggag acgacaattt
     3181 caattttaaa cgctattgag cgttcgggat tacccaactt cattcagatt gcgccaacca
     3241 atgcacaact ctcgggatac gattacattt atgaaatcgt gaaacgtcac gccgataaaa
     3301 tggatgttcc ggtcagtctt catctggatc acgggaaaac tctggaggat gtgaaacagg
     3361 cggtgcgcgc gggctttacc tcggtcatga ttgacggtgc cgcattctct tttgaagaga
     3421 atatcgcttt cacccaggaa gctgtcgatt tctgtaaatc ctatggtgta ccggttgaag
     3481 cggagctggg ggcgattctc ggcaaagaag atgatcatgt cagcgaagcc gattgcaaaa
     3541 cggaaccgga aaaggtgaag acctttgtcg agcgtaccgg ttgcgacatg ctggcagttt
     3601 ctattggcaa cgttcatggc ctggacgata taccgcgtat cgatatcccc ctgctgaagc
     3661 gcattgccga agtctgtccg gtgccgttgg ttattcacgg cggctccggt atcgccccgg
     3721 agatattacg cagctttgtt aattatcgcg tcgctaaagt aaatatcgcc agcgatctgc
     3781 gcaaagcgtt tatcaccgcc gtcggcaaag cgtatgtgaa taatcacaac gaggcgaatc
     3841 tggcacgagt catggcatca gcaaaaaacg ccgtcgagga agatgtgtat agcaaaatcc
     3901 tgatgatgaa tgaggggcat cggttggtta gaaaggcatc ttaagagcca caaaaatata
     3961 gcggcgcaac agaaattgtt gggccgctat aagttataaa tgattcaaac gctcattcag
     4021 tgtttcaata atataggtat taacagagac ttgttgctgc gcagccgcat tattaaggcg
     4081 ttcactcagc gattctggat agcgtaacgt aaaggtctta atcttttctg tacgcgcata
     4141 cggctcaata cccgcagctt tacaatcctc aagatactct agcagggaaa gttccccttc
     4201 tttttgcagc ccctgaatgc tgtcagagac aaaatcacaa taacctgaca agcccaggaa
     4261 tttgccacga aatgcattca gttctggcac gtaagtgata actgcaggtt gcccggcaat
     4321 ctccatagag tttggcgtct tcagtttgat catggtttta cccctatact tagtaaccag
     4381 tcacgcacgc tttctacagc gcctttatcg gtatctggcg acggatgagg ccgatggaaa
     4441 cacgcaacgc tcatatttag tatgaactta caacgcgaac ctcttccttc cttaatttct
     4501 ccgcctaatg ctttaaccag tgactctata tcagaccact tgatcccttg aggaacagga
     4561 gttttaaata tttgatccaa tgtatttttt tgtttcttcc gtaaagcctg taccttggtt
     4621 ctcatggcat gtccttatga cttcacttta tgacatcaat agtatgacat cacagtatga
     4681 agtcaagttt cccttcctta cgccattgtc ccaatcgtct tcctgaatcg cagcagtgcg
     4741 atggtgaaaa acgcgccgcc aattgccatc agcgtcagga actgcggcca gacgatttcg
     4801 aatccggcac cccggtagag gatggcctgc gcgaggctaa caaagtgtgt cgtcggcatg
     4861 gtcagcataa tgtcctgcac catctgcggc atactttcgc gcggcgtgga tccaccagaa
     4921 agcatttgca acggtagcag caccagaatc accagcagcc ccagttgcgg cattgaacgc
     4981 gctatcgtcc ccataaaaat gccgattgac gtggtggcaa acagactgag cgccacgccc
     5041 agcataaaca gcgggatcga gccttcaatc ggtacgccca gtacaccttt caccatcagc
     5101 accagcgata atcccgatac caccagcacc accagcccca tcgaccagat cttcgccatc
     5161 atgatctcaa acggcgttat cggcatcacc agtaaatgtt ccaccgtgcc gtgttcacgc
     5221 tcgcggatca gcgccgatcc ggtcaataca atcgccagca tggtaatgtt gttgatgatc
     5281 gccatcaccc cgccaaacca cgcgggatca aggttcgggt taaagcgcat ccgggtttcc
     5341 agcgatacca acggttcgct gttatcacgg tagcgcgcga caaagctgtt cacttcaccg
     5401 ttgataatat tctggatata cccattgccg gtaaatgcct ggctcatgcg cgtggcatcg
     5461 acgttcacct gaatatccgg ctggcgtccg gcgaggacat cacgctgaaa attaggcgga
     5521 atatttatcg cgaaggtata gcgtccggcg tccagtccgg catccatctc atcggcggtg
     5581 atcatctccg gtggcaaaaa ccacgggcga tagaagctgt taacgatccg gttcgataac
     5641 tgcgattgat ccatatcggc aatggcgatc ggcgcgaggt tcaacgatcc aggcgtaacg
     5701 gtcgctgacg aatacaccga caccgtaaac gagaagacaa tcagcgtcag catcgcttta
     5761 tcaccgagca gactgcgcaa ctctttgata cccagattaa aaatattgcg taaatggcgc
     5821 atcatccctc ctgttttttc agcagcagga tacttaagcc catcaccagc gggatggcta
     5881 tcagcaacgg gataaaaagt tgccacaaat cagtcagatc cagcgccttc gagaacgtcc
     5941 cgcgggcgat agtcagaaaa tgactggtcg ggtaaacctc gccgatccaa cgtccaggcc
     6001 cttccagcga agctaccgga tcgatcatcc cggaaaactg tgtcgccggg atcaatgtga
     6061 taatcgccgt tccgaaaatg gcggcaatct ggcttttcat aaaggtggag atcagcagcc
     6121 ccattccggt ggcaatgatg atatacagca gcgccgccag ggtgagcgtc aggaaactgc
     6181 ctttatgcgg tacgccaaac acaaacaccg acaggccgca gagcaggaaa aagttcagca
     6241 tccccagcgc gatgtacggc agctgtttgc caagcaaaaa ttcgctacgg gtggtggggg
     6301 tcacgtaaag gttgataatc gaccctagct ctttttcccg caccacgcta agggcgctta
     6361 gcattgacgg gatcatcatc agcagaagcg ggatcaccgc cggaacaatc gctggcaggc
     6421 tttttacgtc cgggttatag cgataacgcg tctcaatgtt catcagcccg ctttggctgg
     6481 cgggtgtcga ttgtcggctc gccacatcct gaagccaact ctggtgcatt gcctgcacgt
     6541 aaccttttac cgtttcggca cgactgggca tcgctccgtc gatccagacg ccgagttcca
     6601 caggcgtacc acgcgcgata tcgcgcccga aattgggcgg gatctcaatc gccaccgtga
     6661 tatcgcccgc acgcatccga cgatcaagct cgtcataact ggtgagcggc ggctgttcga
     6721 taaagtaacg ggaaccggag agattgagcg tccacgcctg gctactgacg gtctggtcgc
     6781 ggtcgagcac agcaaagcgc aggttttcca catccatact gatgccgtaa cccattatca
     6841 gcatcaggat caccgttccc atcagcgcca gcgtcgaacg tactggatcg cgtcgcagtt
     6901 ccagcgcttc gcggcggctg tagctaaaca gacggcgcag gctaaatccc tgacgcggcg
     6961 cgtgggtggt gtcgtgtacc acgggcggcg cttcggcttc gttgctctgc cctgccgctt
     7021 cctgcaaata ggcgataaat gcctcttcca gactggcggc tccgcgtttc tcaaccagtt
     7081 cctgcggtgt accgctggca agcacttttc cggcgtgcat cagtgagatg cggtcgcaac
     7141 gttccgcttc gttcatgaag tgggtggaga tgaagatagt cactttgtcc tggcgcgaga
     7201 gatcgaccat caactgccag aacatatccc tcgccaccgg atcgacacca gaagtaggct
     7261 catcgaggat taacatctcc gggcgatgaa tcaccgccac cgccagcgaa agccgctggc
     7321 gaatgccgag cggcaatgac tccggcagaa tatcttcaac gtcgttgagc ttaaaacgct
     7381 cgctcatttc agccactctt gcgggaattt ccgcttccgg gatgtgaaac aaacgggcat
     7441 gtaactcaag gttttgccgc acggtgagtt cgttatagag cgaaaacgcc tgcgacatat
     7501 agcccacccg acggcgggta tcgatatctt ttggatcaac cggttgcccg aacagccacg
     7561 cctcaccttc gctggcgggc agcagtccgg tgagcatttt catggtggtg gatttaccgc
     7621 agccgttcga accaaggaat ccaaaaatct ccccgcgtgg aatgcggaaa ttaacgtgat
     7681 caacggcaac gaaggaacca aaacgcatgg tcagatcgcg cgcttcgatg gcaatctctg
     7741 cgttttcagg ttgatacggt gggatcacta ccgcctgatg cgcctggcgt tgcgcttgcg
     7801 gtaacagatt tataaatgct tcttccagcg tagcgctttg cgtttgctgc cgtagctctt
     7861 cggcgctgcc agttgccagc acttctccgg cattcatcgc taccagccag tcgaagcgtt
     7921 cggcctcttc catataggcg gtggcgacca gcacgctcat attgctctgc cgctgacgaa
     7981 tactgtcgat cagatcccag aactgggcgc gggagagcgg gtcaaccccc gttgttggct
     8041 catcaaggat caacagttcc gggtcgtgga ttaacgcgca gcacagccca agtttttgct
     8101 tcatcccgcc ggagagtttc cctgccggac gatcgcgaaa cggtgctaac cccgtgctgg
     8161 tcagcagctc attaattcgc acttcccgct ccgctttgtc gtgaccgaac aggcgagcga
     8221 aaaaatcgac gttttcatac accgacaagg tgtggtagag gtttttgccc agcccctgcg
     8281 gcatccaggc gatgcgcggg cagacgtcgc ggcgatgcct cgggtcgcgc atatcgccgc
     8341 ccagcaccat cacattgccc tgctcaatga cgcgggcacc ggaaatcaac gacaataagc
     8401 tcgacttccc gacgccatcc ggaccaatca gcccgaccat acagcgggcc ggaatatcga
     8461 gagtaatatt gttcagcgca acggtttttc cataatgctg gctcacgccc gccagttgcg
     8521 cgacaggcgg gacgggaacc agttccagat gcgtcattgc ggcaacctca ccacgaggtc
     8581 gtcaggccac ggaagttctt cattcacccg cacccacgct acgcccggta aaccggtttt
     8641 gacatattcc agatgctgct ggagtaattc cggtgggata cgcgctttga cgcggaacat
     8701 cagtttcagc cgttcatcgc tggtttcgac ggtttttggc gtgaactggg cgacactggc
     8761 gacaaaactg atggtcgccg gaatacgcag atctggcgcg gcatcgagga tcagccgggc
     8821 ttcaccaccc agtttcagcg tgcccgcctg ttcggttggc aggaagaagg tcatatagac
     8881 gtcgctgaga tcgaccatat tcagcacccg accgcctgcc gccagcactt cccctggctc
     8941 ggcaacccga tactgcacgc gtccatcacg cggggctttc agttcgctgt catcgatatc
     9001 tgcggcaatg cgccgttcag tggcttgtgc cgcttcgacg cgggtttgcg cctgaatgat
     9061 attggtacgt gccgcctcta tagcggcttt agaagcagaa acctgagctt ttgccgattc
     9121 cagcgcagct cgggcgctct cagcggcggc gcggtcgtca tccagctgtt gcgcagaaat
     9181 agcccctcgt tgggccagtg aacgggaacg catatgacgt tttgctacgg agtccagttc
     9241 tgcctggcgt tgattaacca gcgactgcgc ggcgcgagtt tcgctttgtc gttgctccag
     9301 caaagcctgc gcggcggcaa cggcgctttg tgcctctttg atttgcgcga tggcttccag
     9361 tcgctgttcc tgcaacacgc gagtatccat cttcgccagc acttcacctt cgcgaacaaa
     9421 ctggccttct ttcaccagaa tggtgtcgat acgcccggca attttgctgg cgatatccac
     9481 ttccgtcgct tcaatgcgcc cattgctgac agcaaagcct tccggcacac ccgccgggcg
     9541 caacagccac cacgccacga cggcagccac cgccagtgcc ccgacaaccc accacgccag
     9601 atggcgctta ctcttatcca taatcggccc gccataatcc ctgtaagcaa acgacgcgtg
     9661 ttacaactgc cgccgaacga cgttcagcgg attcttccat ccgggataag aaggaaacga
     9721 caaatacgac cagcgcgcag agcattgagc gccaacgggg aaggcttagt gaaaacgggt
     9781 agtaatgttg tcatcatcag cattcctggc cgtaaatgaa acacgcatac tcataagtgt
     9841 gatctataaa actggctgac aaaacggtat ccatcctgtt ggatcgcgtt tcacgcctgt
     9901 tcagttgcag gaacagagca ttccttttta cgttttatca acagtctcaa ccagatagta
     9961 acattaatat tcatgttgtc tatggttcag agctgtaaca ttgtgcgcct tctaatcaaa
    10021 ggcataagtc catttctgtt tttttcatgc tggtaagact tccagggagt ggttatctca
    10081 ggccctgcac cagggttaat ttggcaatta tgcatgctga acattcttcc agcatgttta
    10141 ctgtcacggg cgttaagacc taaaactgtc cccgtttcac ctatacattc tcttccacca
    10201 ttaccgttat gtgcaacaac ttgatttata cctaacattt gtgagcatgc taatagtgct
    10261 tcaagtttag gtctgtaatc tgtcgaaaca gaaaaataac gattaaagga tcgacaagag
    10321 atagcccggc tatttttaaa ctttttgcca ttaattacag catggtgctt tttattgata
    10381 attgccgcta attcgaccgg atcgagagca ttggtcattt catcactaga gactgttatc
    10441 ggtcctataa tgtaacgttt tcgctgatca tcaaaagtta tcccatgatg atttgccatt
    10501 attttgtatt tggggccata gaaacataca ggataatctt ttatcccagc gtaggtatcc
    10561 tcatcaagtg gtttcatgcg cgtgaagttc tgcatatagt taccattgca attcgtttca
    10621 tgatttccag ctaatacatg aacattttca ttgatactca ttatgcgttt taacaaatcg
    10681 atgataaatt gatcgccaca gatattacta aatcgatcgc cggttgggtc gccaagaaac
    10741 gtacatggcg tcacagcgtc tttattaata actaaataag gctgaagact ttctaaaact
    10801 atatctcttc tgttcagaat actgattatt gtattaacat tagtgtcaag ttgctcatta
    10861 aagttctccc ttagcaatac gttgagaact tcatcttcta tattaagcaa ctggctaagg
    10921 aaattttctc cttcagccat gattctcaaa tgtcccgttg caaaaagcgc gtacaaaact
    10981 gcaccgactg agccatcggt atcgccaaaa taggttggtc tggtacttgt gggatcaaca
    11041 ggtgaacaat tggagaaata ggttctgtca gcgaactggg ggtttatcat tgtttcgaat
    11101 ttaatatcag atacattgaa ttgcgatatt ttccagtccc agacgggttt gtacgactca
    11161 gtaactgcaa catcattagc atcatcattt actgtcatat tattaagtaa ataaacaatg
    11221 ctgcaccaat taatatcggt attatcaaga tagcagccgt aatttttact attaattgtc
    11281 acaataaact gataattatt atccctacgt agaatatgag cgcgcatgtc cgatagcata
    11341 ctatattttt ctgaatgatt cgtttgtatg gtaaatgata ttttgtcatc tcgaatagat
    11401 tgttggaaac gttctatttg ccaatcatca attttttcca caacttcacc gagcattgtc
    11461 ctggcaatac ttgatatatc atcacctata ccatttgcaa tatacccaaa cagttcttta
    11521 gtgaagttag taacatgctc ttgcaccata tcatttgttg cgattgtcgt tgtactgtcg
    11581 ttagtgcctg ccacagttcc aattttcatc ttttacctca tataattcac cgcgaggaat
    11641 gcatagttaa catttaatct tatgacatac acctcatgtt cgatggaaaa ataattatat
    11701 tttgcacaaa tcattcagcc ttaaagcata atatgaaatt tcttatctca tagcagagaa
    11761 agtccctaaa gattgtagag gcgtcatcag aacatgcctc tacaatcgat taagaaaacc
    11821 tatgaaatac tggcaggatt aaaaagacat accaccgtat tcatggagtt ttaatgagat
    11881 tgaaaacaac taagccctga ggaataaatt attttattat ttttatcacg tgcttccttc
    11941 agggaattta gccccataat accattatca aattgttgat tctgatctac cccgacccca
    12001 tgatgcccat atttttgtct aatgtatttg ctggggtctt cacctttgag tgtctcccca
    12061 tatatgaaga tacgtttacg tgctggtctg aaatcagtag tgttatcaaa ataattagca
    12121 tatacaggtg tgggtttcaa agtattgctt cggattagat ggaatagagg atcatccatg
    12181 tgttctttat gttttttatt aaaaatattg gctaattcct caggatcagt aggatttttc
    12241 atctgattaa atggaacctg caaagcaccg agacaataac atttttttct tcatctctta
    12301 ttattccgtg atgactggtt aaaactttcc tttctgagtc ataatgacaa acatcgagcg
    12361 ttttaattaa gttatacgta tcaccggcag agagtttata attttctagt catgcagtat
    12421 agttaccatt aaaattgatt tcatgatttc tagccagaac gatgacattt ttattaatac
    12481 ggctatcttt attaccctcc atatttcgca tagaatttag caaagtaagg atatatttat
    12541 caccaaaaat agtactaaat ctatcgcctg tatgatcccc aaggaagata caaggggtta
    12601 tcgcatcgtc ctttaaaaaa acatgttgat gcaactcatt caatatca
//
LOCUS       DAZFXT010000128        12524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.128, whole genome shotgun sequence.
ACCESSION   DAZFXT010000128 DAZFXT010000000
VERSION     DAZFXT010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.128"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            76..585
                     /gene="tssB"
                     /locus_tag="ACHKW0_003940"
     CDS             76..585
                     /gene="tssB"
                     /locus_tag="ACHKW0_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HFO7476690.1"
                     /translation="MADSFQNEVPAARVNIKLDLHTGNAKKKVELPLKLLAVGDYSNG
                     KEQRPLSERDKIDINKNNFNSVMAEFSPAVNLTVEDTLSGSGNEQNIALEFKSLKDFE
                     PEQVAKNIPQLRVLLAMRNLLRDLKSNLLDNATFRRELENILKDPTLSSELRDELAKL
                     RRRRTFNPK"
     gene            634..2172
                     /gene="tssC"
                     /locus_tag="ACHKW0_003941"
     CDS             634..2172
                     /gene="tssC"
                     /locus_tag="ACHKW0_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HFO7476691.1"
                     /translation="MSVQQEHATSETATLTTTESGGVYQSLFDKINLTPVSSIQEIDL
                     WQNSETLADASPDERVTAAIHVLLSCLAKSGENVVKLDKSLLDFHIDDLDQKISKQLD
                     AVMHHPEFQKVESLWRGTWFVVQRTDFRKNVRIELLDISKEHLRQDFDDSPEIIQSGL
                     YRHTYIQEYDTPGGEPVASLISSYEFDNSPQDIALLRNISRVSAASHMPFIGSVGPKF
                     FLKNSMEEVAAIKDIGNYFDRAEYIKWKSFRDTDDSRYVGLVMPRVLGRLPYGPDTVP
                     VRSFNYVEEVKGPDHEKYLWTNASFAFAANMVKSFVNNGWCVQIRGPQAGGAVADLPI
                     HLYDLGTGNQVKIPSEVMIPETREFEFSNLGFIPLSYYKNRDYACFFSANSAQKPALY
                     DTADATANSRINARLPYIFLLSRIAHYLKIIQRENIGTTKDRRVLELELNTWVRTLVT
                     EMTDPGDELQASHPLRDGKVIVEDIEDNPGFFRVRLFAVPHFQIEGMDINLSLVSQMP
                     KAKA"
     gene            2190..3527
                     /gene="tssK"
                     /locus_tag="ACHKW0_003942"
     CDS             2190..3527
                     /gene="tssK"
                     /locus_tag="ACHKW0_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HFO7476692.1"
                     /translation="MKIHRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGI
                     ANVLFDRDALMSGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLHALTNDSAVVLLAL
                     PLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
                     YITCPLARLVRDVQGNWTQDEGYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMR
                     RESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLT
                     FSLEHNVSAVPPYVHESPSTVFPPLFSLLSELLEASLPSRVIALDLGSLPGNRWKADL
                     HDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTS
                     VPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT"
     gene            3524..4189
                     /gene="tssL"
                     /locus_tag="ACHKW0_003943"
     CDS             3524..4189
                     /gene="tssL"
                     /locus_tag="ACHKW0_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, short
                     form"
                     /protein_id="HFO7476693.1"
                     /translation="MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVE
                     GVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQAIWMKDPLQAHFFNTLQ
                     AGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPVAPERDQLISTLNGLVA
                     PFGVLPETAVLNVPLSPRQHPLLHSPFFWLVTLTLLLAGVWWGLHHWLNVLVDELLPQ
                     SLR"
     gene            4202..5854
                     /locus_tag="ACHKW0_003944"
     CDS             4202..5854
                     /locus_tag="ACHKW0_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HFO7476694.1"
                     /translation="MRNTLKQAVVLWGMVLLLVLWSVFISPSGVLIWAGAAAIVLTVA
                     ALLIYRRRQAWTEMTGDAGLLSLPPETYRQPVVLVCGDMSAHLFTDSPVRQVSEGLYL
                     HVSDEEQLVAQAERLLTLRPAWASQLAVAYTVMPGMYRDAAVLTGQLRRFAHSMATVR
                     RRAGVNVPWLLWSGLSGSPLPERANSPWFICTGGEIHVATSAETASPAQWLTQTSTQE
                     RSQQLCYLLKAESLMQWLNLNMLAALNGPETKCPPLAMAVGLVPSLPAVDNNLWQLWI
                     TARTGLTTDIADTGTDATLPFPDALLRRLPRQSGFTPLRRACVTMLGITTVAGIAALC
                     LSATENRQLLRHIGDDLHQFYAVPAEEFITKARRLSVLKDDAIMLDGYYREGEPLRLG
                     LGLYPGEQIRQPVLRAIRDWRPPEQKMEVTASLQAQTVRLDSMSLFDVGQARLKDGST
                     KVLVDALVNIRAKPGWLILVAGYTDATGDEKSNQQLSLRRAEAVRNWMLQTSDIPATC
                     FAVQGLGESQPAATNDTPQGRAVNRRVEISLVPRSDACQDVK"
     gene            5912..6403
                     /locus_tag="ACHKW0_003945"
     CDS             5912..6403
                     /locus_tag="ACHKW0_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7476695.1"
                     /translation="MAIPAYLWLKDDGGADIKGSVDVQGREGSIEVVALDHDVYIPTD
                     NNTGKLTGTRTHKPFIFTKEIDASSPYLYKAVTTGQTLKTAEFKFYRINDAGQEVEYF
                     NITLDNVKLVRVAPLMHDIKEPSKEKHNHLERIEFRYEKITWTYKDGNIIHSDSWNER
                     PSA"
     gene            6594..9239
                     /gene="tssH"
                     /locus_tag="ACHKW0_003946"
                     /gene_synonym="clpV"
     CDS             6594..9239
                     /gene="tssH"
                     /locus_tag="ACHKW0_003946"
                     /gene_synonym="clpV"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HFO7476696.1"
                     /translation="MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLK
                     LLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRSVRERPQLSEPLTALIRQAWLI
                     ASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
                     RQPQATEPLTSAQPESATTDAPAKTLTGKQDDALLAVLNRFTEDVTEKARSGRIDPVF
                     GRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRT
                     LDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAAN
                     LLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTASLMLRGLKA
                     RYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLT
                     QLQARLTALDIEREAIEQDSVFYPETSPERLAELTDLRDELQAEAGHLEAQYQQEKRL
                     AQQIMALRQEGADSTDLQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLRD
                     EQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKT
                     ETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRP
                     YSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQL
                     LEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVVR
                     RLRQHYGLETEINDSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAAHR
                     KPAQLRLGWDEEDGIVLEFATEEMQ"
     gene            9251..11758
                     /locus_tag="ACHKW0_003947"
     CDS             9251..11758
                     /locus_tag="ACHKW0_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system Vgr family protein"
                     /protein_id="HFO7476697.1"
                     /translation="MGIQNFDHSHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFT
                     SSDKAITPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEV
                     RLEPRMALLARSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPSREQVMQ
                     YGEDDLTFISRLLSEVGIWFRFATDARLKIEVVEFYDDQSGYERGLTLPLRHPSGLFD
                     GETEAVWGLNTAYSVVEKSVTTRDYNYRTATAEMMTEQHDATGGDNTTYGEAYHYADN
                     FLQKGDKEAAESGAFYARIRHERYLNEQAILQGQSTSSLLMPGLEIRVQGDDAPAVFR
                     KGVLITGVTASAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKND
                     IYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAF
                     EEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEY
                     GGKSQLNLGHLVDAGKQQRGEGFELRTDMWGAVRAKKGIFISADAQDKAQGQVREMAP
                     AMAILDGAQSQMQSLSTDAQTTNADPADLSSQIALLQQSVKDLTQAVILLSAPKGVAI
                     ASGEHLQLAASKNVIANAGNNADIGVVKNMFIGVGQALSVFVRKAGIKLFANKGAVSV
                     QAQNDLMELLAQKSIEITSTEDEIKITAKKKITLNGGGSYIRLDACGIEAGTPGEYNV
                     KAGYYGRKPKAKLTPELMAFPVIKSEDFNQSFILLDENTGQPLINWPYELELESGLKM
                     SGITDENGNTELISSDKEEVVNISVFEPDEFLDDDIN"
     gene            11780..12373
                     /locus_tag="ACHKW0_003948"
     CDS             11780..12373
                     /locus_tag="ACHKW0_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7476698.1"
                     /translation="MADKSEKKKETTNKSAKKTLTKKSEKLTPAKDTAPKQVNVDPAI
                     RLKQIRQLVRNNNKSIMGEDYIICQIYKESRFKQFAGKNKHNAKGLMQMQRNAVRQVF
                     KYRQQKIKGRMTTDKETNEAFANADTFYKSDKIFDEKENIKIGTEYLQYWIDKEATIE
                     EAYRGTDEAYYSVIKPCAEKLAKDPDNIQILMEGIGR"
BASE COUNT         3041 a         3347 c         3575 g         2561 t
ORIGIN      
        1 ttaaatcacc cgataatccc gtgaatataa ttatgtcaat aaccatcaga aaaagtggat
       61 gatgaggaaa aggatatggc cgacagtttc cagaatgaag ttcccgccgc tcgtgtaaat
      121 atcaagcttg acctgcatac aggcaatgct aaaaagaaag ttgaactccc cctcaagctt
      181 cttgctgttg gcgattacag taacggaaaa gagcaacgtc cgctgtccga acgggacaag
      241 attgatatca ataaaaacaa cttcaacagt gtcatggctg agttttcgcc tgcggttaat
      301 ttaacagtag aagacacact aagcggaagc ggtaatgagc aaaatatcgc gcttgaattt
      361 aaaagcctga aagattttga acccgaacag gtagcaaaaa atatcccaca gcttcgtgta
      421 ttactcgcta tgcgcaattt attacgtgac ctgaaatcca atctgctgga taacgcaacg
      481 tttcgccgtg aactggaaaa tattctgaaa gacccaacgc tcagttcaga actgcgtgat
      541 gaactggcga aattgcgccg caggagaacg tttaacccga agtgatattc aggttaaacc
      601 ggagaagaaa ggatcactga aaaggaaatg ctgatgtctg tacaacaaga acatgccacc
      661 tctgaaactg caacactgac caccactgag tccggcggcg tttatcagtc cctgtttgat
      721 aaaattaatt taaccccggt gtcttccatt caggaaatag atttatggca aaacagcgaa
      781 acactggcgg atgcctcacc cgatgagcgc gtgacagcgg cgattcacgt tctgctttcc
      841 tgtctggcga aatcaggcga gaacgtggtc aagctcgaca agagcctgct ggattttcat
      901 atcgacgatt tggatcagaa aatcagtaag cagcttgatg cggtcatgca ccatcctgaa
      961 ttccagaaag tcgagtcgct gtggcgcggc acatggttcg tcgtacagcg cactgatttt
     1021 cgcaaaaatg tcagaattga attgctggat atcagcaaag agcatctgcg tcaggatttc
     1081 gacgactccc cggaaatcat tcagagtggt ttatatcgtc atacatatat tcaggagtac
     1141 gatacgccgg gtggcgaacc tgttgcctca ttaatttcca gctatgaatt tgataacagc
     1201 ccgcaggata ttgccctgct gcgcaatatt tccagagtgt ctgccgcttc ccatatgcct
     1261 tttatcggtt ctgtcgggcc gaaattcttc cttaaaaatt cgatggaaga agtcgccgcg
     1321 attaaagata tcggcaacta ctttgaccgc gcagaatata ttaaatggaa atcgtttcgt
     1381 gataccgatg acagccgcta tgtgggatta gtgatgccgc gagtgctggg ccgtctgccc
     1441 tatgggccgg acacggtgcc ggtacgcagc tttaactatg tggaagaagt caaaggcccg
     1501 gatcacgaaa agtatctctg gacaaacgcc tcgttcgcct ttgccgccaa tatggtgaag
     1561 agctttgtga ataatggctg gtgcgtgcag atccgcggtc cacaagcagg tggcgcagtg
     1621 gccgatctgc ccatccatct ttacgatctc ggcaccggca atcaggtcaa aattccgtcc
     1681 gaagtgatga tcccggaaac ccgcgaattt gaattttcca accttggctt tattccgctc
     1741 tcttattata agaatcgcga ttacgcctgc ttcttctcgg caaactctgc ccagaaaccg
     1801 gcgttgtatg ataccgctga cgccaccgcc aacagccgta ttaacgcccg tctgccctat
     1861 atcttcctgc tgtcccgcat tgcgcattac ctgaaaatca tccagcgcga gaatatcggt
     1921 accaccaaag accgccgcgt actggaactg gaactgaaca cctgggtccg cacactggtg
     1981 acggagatga ccgatccggg tgatgaactg caggcgtctc acccgctgcg cgacggtaag
     2041 gttatcgtgg aagatattga ggacaatccg ggcttcttcc gcgtcagact ctttgccgtg
     2101 ccgcatttcc agattgaagg gatggatatc aacctgtcac tggtttccca gatgccgaaa
     2161 gcgaaagcct gaccgggaag tggtgtgata tgaaaattca tcgaccactg tgggctgagg
     2221 ggacattcct gtcctctcag cagtttcagc agcaggcccg ctgggaagcg ttcagcaatg
     2281 acagcattgc gcaactttgt attcgtcatc cgtgggggat cgcgaatgtg ctctttgacc
     2341 gcgatgccct gatgtccgga aagctgaaaa cgcaggcagt acgcctgcgt tttgctgatg
     2401 gcacgctgat cgattctgac gtgagcgatg ttctgcctct cgcctgcgat ctgcacgcgc
     2461 tgacgaacga cagcgcggtt gttctgctgg cgctgccgct tgcccatggt aacggcggta
     2521 atctcggtca gggtgagcag actgaaagac cgctacgcta tcgtcaggaa tggcaaaagg
     2581 tacaggatat ctacggcagt gacagtgaag atatggccgt tgagcgtcat gcgctgtccc
     2641 tgcgctttgc tcatgataac aatcaggatt acatcacctg cccactggcg cgtctggtgc
     2701 gggatgttca gggcaactgg acacaggatg agggctatat tccgccgtta ctggcgttta
     2761 atgctcatga cgggttggta cagcgtctgg acacgctgct gttgcagcta cgggccaaat
     2821 gtcagcgcct gatggcgatg cgtcgggaaa gcaaccagcg tatggcggac ttcgccgtcg
     2881 ccgatgtctc cctgttctgg ttgcttaacg cgctgaactc cgccgagccg gtgctgagtg
     2941 atttcctgcg ctacccggcg gttcatcccg aactggtctg gcgggagctg gcgaggcttg
     3001 ccggagcgct gctgacgttt tcccttgaac ataacgtcag cgccgttccg ccctatgtcc
     3061 acgaatcgcc gtccactgtc tttccgccac tgttctccct gctcagcgaa ctgctggaag
     3121 caagcctgcc gtcacgggtg atcgcgctgg atctgggatc cctgccgggc aaccgctgga
     3181 aagccgacct gcacgatccg cgcctgcggg aagaggccga tttttacctg tccgttcgtt
     3241 cctcactgcc tgcgcatcag gtactgcacc agctaccgct ggtctgcaaa atcggtgcgc
     3301 ctgatgacgt cacgctgctt atcaacgtgg cgctgaatgg tgttccactg gtgccactga
     3361 cctcagttcc ggcagcgctg ccactgcgcc tggagaatca gtattttgcg ctggatatgc
     3421 acagtgacgc ggctaaatcg atgctggagt ccgggtgctg catgatttat gcgccaggaa
     3481 ccatgggcga cctgaaacct gaactgtttg cggtgctgcg cacatgaaaa aagagacgga
     3541 tatcaatatc gacgcgctgc tgcgcgacac gtttctgacg gtggtggaac tgcgtcaggg
     3601 aaccaccgtg cgtcacggta tggagctgta tcgccactgc cagcggcagg tggagggggt
     3661 ccgcgaacgc ctgaaagatg ccgggttcag ccgggagaac gtggagcaca ttacttacgc
     3721 acagtgcgcc ctgctggacg aaacagtcct gagtcgcagc ggcatggacg acggtcaggc
     3781 catctggatg aaggatccac tccaggcgca tttcttcaac accctgcagg caggcgagct
     3841 gctgtacgag cggatgaagc aggtgttgca ggagcccgcg cctgcacagg cagtgctgac
     3901 ctgctttcac cgtgtcctgc tgctgggatt tcgcgggcgt tatcaggatc cggtggcccc
     3961 ggagcgtgac caacttattt cgacgctcaa cgggctggtc gcgccatttg gcgtgctgcc
     4021 ggaaacggcg gtactgaacg tgccgttatc cccccgtcag caccctttgc tgcattcgcc
     4081 gtttttctgg ctggtaacgc tgacgctgct actggccggt gtctggtggg gactgcacca
     4141 ctggctgaac gtgctggtgg atgaattgct gccgcaaagc ctgcggtaaa gggagtcgtg
     4201 agtgaggaac acgctgaaac aggccgtcgt gctgtgggga atggtgttac tgctggtgct
     4261 gtggtcggtg tttatcagtc cgtctggcgt actgatatgg gccggggcgg cggctatcgt
     4321 tctgacggtt gccgcgttgc tgatttaccg gcgcaggcag gcgtggacgg agatgaccgg
     4381 cgatgccggg ttgttatcgc tgccgccgga aacgtaccga caaccggtag tgctggtctg
     4441 tggcgatatg tcggcgcatc tgttcactga cagcccggtc cggcaggttt cagaagggct
     4501 gtatctgcat gtttctgatg aagaacagct tgtggcgcag gcggagcgat tactgaccct
     4561 tcgcccggcg tgggcatcgc agcttgccgt ggcatatacc gtcatgcccg gcatgtaccg
     4621 ggatgcggcg gttctgaccg gacaactgcg ccggttcgcc cacagtatgg cgacagtgcg
     4681 tcgccgggcg ggcgtaaacg tcccctggct tctctggagc gggctgtccg gctcgccgtt
     4741 gccggagaga gcgaattcac cgtggtttat ctgtaccggc ggcgaaattc atgtagcgac
     4801 gtctgctgaa accgcctccc ccgcgcagtg gctcacgcag accagcacac aggagcgcag
     4861 ccagcaactc tgttacctgc tgaaagccga aagcctgatg cagtggctga atcttaatat
     4921 gctggcggca ctgaacggcc cggaaacgaa atgtccgcca ctggcgatgg ccgtggggct
     4981 ggtcccctcg ttgcctgcgg tggataacaa cctgtggcag ttgtggatca ccgccagaac
     5041 cggcctgacg acggatatcg cggacaccgg cacagacgcc acgctgccgt ttccggatgc
     5101 cctgttacgg cggttgccgc gtcagtcggg ctttaccccg ctgcgacgag cctgcgtgac
     5161 catgctgggc atcaccaccg tggcgggtat cgccgcgctg tgtctgtcag ccacggagaa
     5221 tcgccagtta ttacggcata tcggtgatga tctgcaccag ttttatgccg ttccggcgga
     5281 ggagtttatc accaaagccc gtcgtctgtc ggtgctgaaa gacgatgcga tcatgctcga
     5341 tgggtattac cgggaaggag agccactgcg tctcggtctg gggttatacc ccggtgaaca
     5401 aatccgccag ccggtattac gcgccattcg tgactggcgt ccgcctgagc aaaaaatgga
     5461 agtgacagcc tcgcttcagg cgcagaccgt gcgtcttgac agtatgtcgc tgtttgacgt
     5521 cggacaggcc cgcctgaaag acggctcgac aaaagtgctg gtggacgcac tggtgaacat
     5581 ccgggcaaaa ccgggctggc tgatcctcgt ggccggatat accgacgcca ccggcgacga
     5641 aaaaagcaat cagcagttat cgctgcgacg tgccgaagcg gtgcgcaact ggatgctgca
     5701 gaccagcgac atcccggcca cctgttttgc cgtacaggga ctgggcgaga gccagcctgc
     5761 ggcgaccaac gatacgccac agggccgggc agtcaaccgg cgcgtcgaaa tcagtcttgt
     5821 tccgcgttct gacgcctgtc aggacgtgaa ataaaacata ccgccggaag aaggcggtgc
     5881 ttcaatcaca ctaacaagga gagtaattct catggctatt cctgcttatc tctggctgaa
     5941 agatgacggc ggcgcggata tcaaaggttc cgtggacgtt caggggcgcg aaggcagcat
     6001 cgaagtggtg gcgctggatc acgatgtgta catcccgacc gacaacaaca ccggcaaact
     6061 gaccggtact cgcacccata aaccgttcat ttttaccaaa gaaatcgatg cgtccagccc
     6121 gtatctctac aaagctgtga ccaccggtca gaccctgaaa acggcggaat ttaagtttta
     6181 ccgcatcaac gacgccggtc aggaagtgga gtacttcaac atcacgctgg ataacgtcaa
     6241 gctggtcaga gtcgctccgc tgatgcacga catcaaggag ccttccaaag agaagcataa
     6301 ccacctggaa cgcattgagt tccgctacga aaaaatcacc tggacttaca aagacggcaa
     6361 catcattcat tccgactcgt ggaatgaacg tccttccgcc tgataactgc ggcagacagg
     6421 aaactgtctg cctttctgtt gtgtgctttg cccagtgccg caggcaccgg tactgagcaa
     6481 agcataccca caatctggtg atacctgatg ggcctgagtg aggtaacggc tatgggcggt
     6541 agcgtacctg aacgcagatc gcaggaatac agaacacgat aaggaaacct tctatgaccg
     6601 gaaatcaccc cgccgcgctg ctgcgtcgcc ttaacccgta ctgtgcacgg gcgctggacg
     6661 ctgccgcctc actgtgtcag acccgcgccc atgcggagat aaccattgaa cactggctgc
     6721 tgaaactgct ggagcaggga gaaggcgata tcacggtgat tgcccgccgc tatgaatggg
     6781 atatcgacac gctctggcag tctctgctgg cacatctgga caccttaccc cgctcggtcc
     6841 gcgaacgtcc gcagctttct gaacccctga cggcgcttat caggcaggcg tggctgattg
     6901 cctcgctgga aggcgacgac ccgcaaatcc gcagtcagca cctgctgatg gcgctgacag
     6961 aaaaaccgat gctgcccgcc tgtaatgacc tgtgggtatt gctgagcctg agccgcgtgc
     7021 agcttgagcg gctgcgcccc ctgctggatg cgcagtcgga tgaatgtccg gcacgtcagc
     7081 cacaggccac cgaaccgctg acatccgcgc aaccagagtc ggcaacgacg gacgcaccgg
     7141 caaaaacgct gacggggaaa caggatgacg ccctgctggc ggtacttaac cgctttaccg
     7201 aagacgtgac ggaaaaagcc cgcagcgggc gaatcgaccc ggtattcggg cgcgacaccg
     7261 aaattcgcca gatggtcgat atcctctccc gtcgccgcaa aaacaacccg attctggtgg
     7321 gagaaccggg cgtgggcaaa accgcgctgg tggaagggct ggcgctgcgt atcaccgaag
     7381 gcaacgtgcc ggacagcctg aaaacggtgc atatccgcac actggacctc ggtctgttac
     7441 aggctggcgc gggcgttaaa ggtgaatttg agcagcgact gaaaaacgtg attgatgcag
     7501 tacagaaatc accggagccg gtactgctgt ttattgatga agcccatacc attatcggcg
     7561 cgggtaatca ggcgggcggc gcggatgcgg cgaacctgct gaaaccggcg ctggcaaggg
     7621 gcgagctgcg caccatcgcc gccacaacgt ggagcgaata caaacagtat tttgagcgcg
     7681 acgccgcgct ggaacgccgc ttccagatgg tgaaagtcga cgagccggat gatgacaccg
     7741 ccagcctgat gctgcgggga ctcaaggccc gctatgcaca gcaccacggc gtacatatgc
     7801 tggacagcgc cattcagacc gccgtgcgcc tgtcgcgccg ctatctgacc ggacgccagt
     7861 tgccggacaa ggcggttgat ttgctggata ccgccggggc aagagtccgc atgagcctcg
     7921 acaccctgcc ggaaccgttg acgcagcttc aggcgcgtct gaccgcgctg gatattgagc
     7981 gggaagcgat tgagcaggac agcgtgtttt atcccgaaac cagcccggag cggctggcgg
     8041 aactgaccga tttgcgtgat gagctacagg cagaagccgg gcatctggaa gcgcagtatc
     8101 agcaggaaaa gcggcttgcg cagcagatta tggcgttgcg tcaggaaggg gcagacagca
     8161 ctgatttgca acagcaactg cgaacgcatc agggctttgc accgctgctg gcgctggatg
     8221 tggacgcccg cgccgtcgcc actgtggtgg cggactggac cggcatcccg ctctcttccc
     8281 tgctcaggga cgagcagagc gacctgctca gtatggaaca aagccttgaa aaccgcgttg
     8341 tcgggcaaag cccggcgctc tgcgccatcg cacagcggct gcgggcggct aaaaccggcc
     8401 tcacgccgga gaacggcccg cagggggtat tcctgctgac cggccccagc ggcaccggta
     8461 aaaccgaaac tgcgctcaca ctggccgaca ccctgtttgg cggtgaaaaa tcccttatca
     8521 ccatcaacct ctccgaatat caggagccgc ataccgtctc ccagcttaag gggtctcctc
     8581 cgggctacgt tggctacggt cagggcggcg tactcaccga agccgttcgc aaacgcccgt
     8641 acagcgtggt gctgctcgac gaagtggaaa aggcgcatcg cgacgtgatg aacctgttct
     8701 atcaggtatt tgatcggggc tttatgcgcg acggcgaagg ccgggaaatt gacttccgca
     8761 acaccgtgat tctgatgacg gctaatctgg gcagtgacca catcatgcag ctgctggagg
     8821 aaaaaccgga cgccacggac gcagacctgc atgaactgct gtaccccctg ctgcgcgagc
     8881 acttccagcc tgcactgatg gcgcgttttc agacggtgat ttaccgccct ctgggacagg
     8941 aagcgatgcg cgccattgtg gaaatgaaac tggcgcaggt ggttcgtcgt cttcgtcagc
     9001 actatgggct ggaaacggaa atcaacgaca gcctgtatga cgcgctgacc gccgcctgcc
     9061 tgctgccgga caccggggcg cgtaatatcg acagcctgct gaaccagcaa atcctgccgg
     9121 tcttaagcca gcagttgctg gcgcaacagg ccgcgcaccg taaaccagcc cagctacggc
     9181 ttggctggga tgaggaagac gggattgtac tggagttcgc tacggaagag atgcaataac
     9241 ggaggctgtc atgggtattc agaatttcga tcattcacac cacaagctca aaattcgtgg
     9301 cctgcaaagt ccggtggatg tgctgacctt cgaaggccgc gaacaactga gtaccccgtt
     9361 ccgctacgac attcagttta ccagcagcga taaagccatc acgccggagt cggtgctgat
     9421 gcaggacggc gcattcagcc tgacggcacc gccggtacag gggatgccgg tacagactgc
     9481 cctgcgcacc ctgcacggcg tcatcaccgg ctttaaacac ctgtcatcgt cacaggatga
     9541 agcccgttac gaagtgcgcc ttgaaccccg aatggcgctg ctggcccgca gccgccagaa
     9601 cgccatttac cagaaccaga cggtgccgca aatagtcgaa aaaatcctgc gcgagcggca
     9661 ccagatgcgc ggtcaggact tcgtgtttaa cctcaaaagc gaatacccgt cccgtgaaca
     9721 ggtgatgcag tacggtgagg acgacctgac gtttatttca cgcctgctgt cggaagtggg
     9781 tatctggttc cgctttgcca ccgacgcccg gctgaaaatt gaggttgtcg agttttacga
     9841 cgaccagagc ggctatgagc gcggactgac gctgccgctt cgccatcctt cagggctgtt
     9901 tgatggtgaa acggaagccg tatgggggct gaacaccgca tacagcgtgg tggaaaaaag
     9961 cgtcaccacg cgggactaca actaccgcac cgccacagca gagatgatga cagagcagca
    10021 tgacgccacc gggggcgata acaccaccta tggcgaagcc taccactacg ctgacaactt
    10081 cctgcaaaaa ggcgacaagg aggcagcgga aagcggcgcg ttttatgccc gtatccgtca
    10141 cgaacgctac ctgaatgaac aggccattct gcagggccag agcaccagtt cactgctgat
    10201 gccggggctg gaaatcaggg ttcagggcga cgacgcgcct gcggtattcc gcaagggcgt
    10261 gctgattacc ggcgtgacgg ccagcgcggc ccgtgaccgc agctatgaac tgacgttcac
    10321 ggcgataccc tactcagaac gctacggcta tcgcccggcg cttatccccc gccccgtaat
    10381 ggccggaaca ctcccggcgc gggtcaccag tacggtgaag aacgacatct acgcccatat
    10441 cgataaggac gggcgctacc gggtcaatct cgactttgac cgtgatacgt ggaagccggg
    10501 ctacgaaagc ctgtgggtac gccagtcgcg cccgtatgcg ggagacacct acggcctgca
    10561 cctgccgctg ctggcgggaa cggaagtcag catcgccttt gaggaaggca acccggaccg
    10621 accgtatatc gccggggtaa aacacgactc ggcacacacc gaccatgtga ccattcagaa
    10681 ctacaagcgc aacgtgctgc gcaccccggc gaacaacaaa atccgcctcg atgatgagcg
    10741 cggcaaagag catatcaagg tcagtaccga gtacggcggc aagagccagt tgaatctggg
    10801 tcatctggtg gatgccggaa aacaacagcg cggggaaggc tttgaactgc gtaccgatat
    10861 gtggggcgcg gtgcgggcga agaaagggat ttttatcagc gccgatgcgc aggataaggc
    10921 gcaggggcag gtgcgcgaaa tggctccggc gatggcaata ctggatggcg cacagtcaca
    10981 gatgcagtcg ctgtccactg atgcgcagac gacgaatgcc gatccggcag atctgtcatc
    11041 gcagatagcg ctgttgcagc aaagtgtaaa agatctgacg caggccgtga tcctgctaag
    11101 tgcacccaag ggcgtggcga tcgccagcgg cgagcacctg caactggcgg cgtcgaagaa
    11161 tgtgatagcg aacgcaggca acaacgcaga tatcggcgta gtaaaaaata tgtttatcgg
    11221 tgtgggtcag gcgctgagcg tgtttgtgcg taaagcgggc atcaagctgt ttgccaataa
    11281 aggggcggtc tcggtacagg cacagaacga tctgatggaa ctgttggcac agaaatcgat
    11341 agagatcacc agtacggaag atgagatcaa gatcaccgcg aagaagaaaa tcacccttaa
    11401 cggcggtggc agctacatcc ggctggatgc ctgcgggata gaagcgggaa cgccgggaga
    11461 atataacgtt aaggccggat attacgggcg taaaccaaag gcgaagctga cgccggagct
    11521 gatggcattt ccggtgatta agtctgagga ttttaatcag agctttattt tgctggatga
    11581 aaatactggg caaccactta ttaattggcc ctatgaacta gaactggaaa gtgggctaaa
    11641 aatgagtggg attactgatg aaaatggaaa tacagaacta atatcctctg ataaggagga
    11701 ggtcgttaat attagtgtgt ttgaacccga tgagttttta gacgatgaca ttaattaaga
    11761 catatagcga gaatataaca tggcagataa atccgaaaag aaaaaagaaa ccacaaataa
    11821 atcagcaaaa aaaacactaa cgaagaagag cgaaaaatta actccagcaa aagacaccgc
    11881 accaaaacaa gtgaatgtag atccagctat aagattgaaa caaattcgtc aacttgtccg
    11941 taataataat aaatccatca tgggagagga ttatattatt tgtcaaatct ataaagaatc
    12001 tagatttaag caatttgcag gtaagaataa acataatgcg aaaggcttaa tgcaaatgca
    12061 acgaaatgca gtgaggcagg tttttaaata tcgacaacaa aaaataaaag gaagaatgac
    12121 gactgataaa gagacaaatg aagctttcgc taatgctgat acattttata aatctgataa
    12181 aatatttgat gaaaaagaaa atattaagat tggcacagag tatttgcaat actggataga
    12241 taaagaagca acaattgagg aggcatatcg tggaacagat gaagcatact attctgttat
    12301 aaaaccttgc gctgaaaaac tggctaaaga tcctgataac atacaaatac taatggaggg
    12361 aataggaaga tagtatgcgc attatgttaa tattactttg tttttttctt tcccctgttt
    12421 ttcaggcgat aagattaaaa tcgtgaaaac ctgtcaactg aaaaaaaaat gaaagtgtgc
    12481 agttgctttc ccttagaacc attgacggtg atacattata tcta
//
LOCUS       DAZFXT010000129        12190 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.129, whole genome shotgun sequence.
ACCESSION   DAZFXT010000129 DAZFXT010000000
VERSION     DAZFXT010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.129"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            267..1037
                     /gene="yjjP"
                     /locus_tag="ACHKW0_003949"
     CDS             267..1037
                     /gene="yjjP"
                     /locus_tag="ACHKW0_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HFO7476699.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1028..1501
                     /gene="yjjB"
                     /locus_tag="ACHKW0_003950"
     CDS             1028..1501
                     /gene="yjjB"
                     /locus_tag="ACHKW0_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HFO7476700.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1608..2147
                     /gene="dnaT"
                     /locus_tag="ACHKW0_003951"
     CDS             1608..2147
                     /gene="dnaT"
                     /locus_tag="ACHKW0_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HFO7476701.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2150..2887
                     /gene="dnaC"
                     /locus_tag="ACHKW0_003952"
     CDS             2150..2887
                     /gene="dnaC"
                     /locus_tag="ACHKW0_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO7476702.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2936..3430
                     /gene="yjjA"
                     /locus_tag="ACHKW0_003953"
     CDS             2936..3430
                     /gene="yjjA"
                     /locus_tag="ACHKW0_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein YjjA"
                     /protein_id="HFO7476703.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQMLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3684..5975
                     /gene="opgB"
                     /locus_tag="ACHKW0_003954"
     CDS             3684..5975
                     /gene="opgB"
                     /locus_tag="ACHKW0_003954"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HFO7476704.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6115..7137)
                     /gene="lgoD"
                     /locus_tag="ACHKW0_003955"
     CDS             complement(6115..7137)
                     /gene="lgoD"
                     /locus_tag="ACHKW0_003955"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HFO7476705.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYQFATLAETYERDVINNRELI
                     KGVITF"
     gene            7276..8190
                     /gene="lgoR"
                     /locus_tag="ACHKW0_003956"
     CDS             7276..8190
                     /gene="lgoR"
                     /locus_tag="ACHKW0_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HFO7476706.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8405..9766
                     /gene="lgoT"
                     /locus_tag="ACHKW0_003957"
     CDS             8405..9766
                     /gene="lgoT"
                     /locus_tag="ACHKW0_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7476707.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLIVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9815..11479)
                     /gene="tsr"
                     /locus_tag="ACHKW0_003958"
     CDS             complement(9815..11479)
                     /gene="tsr"
                     /locus_tag="ACHKW0_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004888 - transmembrane signaling receptor
                     activity [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HFO7476708.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(11598..12044)
                     /gene="hpaR"
                     /locus_tag="ACHKW0_003959"
     CDS             complement(11598..12044)
                     /gene="hpaR"
                     /locus_tag="ACHKW0_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HFO7476709.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
BASE COUNT         2971 a         3213 c         2946 g         3060 t
ORIGIN      
        1 cttgttaaca ttaaaaaacg tgcccggtta tctattatcc tgattcgtaa aggccaaaca
       61 ataaattcat tatgatgata atattcatgt gatagtttgt ttgcggcgag agataattcg
      121 ctttttatca ccgagcttct cccgataacc aggattaatc agcgcaattt tgatcttaac
      181 ctaccgcaaa acaaccgtgc ttcatgatac aaatggattc tcattatctg aacaatacgc
      241 agcacgtcta tgacaaaggg cgagttatgc aaactgagca acagcgagcc gtaacacggc
      301 tttgtatcca gtgtggatta tttcttctgc aacatggtgc ggaaagcgcg ttggttgatg
      361 agctttcctc acgactgggt cgggcgctgg gaatggacag cgttgaaagt tctatctctt
      421 cgaacgccat agtgctgaca accattaaag atgggcaatg cctgacatcg acacgtaaaa
      481 atcacgatcg cggcattaat atgcatgtgg tgactgaagt ccagcacatt gtgattctgg
      541 cggagcacca tctgctggat tacaaaggcg tagagaaacg atttagccaa attcagccat
      601 tacgttaccc aagatggctg gtagccttaa tggttggcct ttcctgcgcc tgtttctgta
      661 aactcaataa aggtggctgg gatggtgccg tcatcacctt ctttgccagt acggccgcga
      721 tgtatatccg ccagctactg gcacaacgtc atcttcatcc acagatcaac ttttgcctca
      781 ccgctttcgc cgccaccact atttccggtt tgcttttgca acttcccacc ttcagcaata
      841 cacccaccat tgcgatggcc gccagcgttc tgctgctggt gccgggattt ccgttgataa
      901 atgccgtcgc cgatatgttt aaaggccaca tcaataccgg actggcacgc tgggcgatcg
      961 ccagtctgct gacactggct acctgcgtcg gcgtggtgat ggcactgacg atttgggggc
     1021 tacgcggatg ggtgtgatcg agtttctgtt tgcattggcg caggatatga tcctcgccgc
     1081 cattccagcg gtcggctttg cgatggtgtt caacgttccc gttcgggcat tacgctggtg
     1141 tgcgctgctt ggcgcgattg gtcatggttc acgaatgatc ttaatgacca gcgggctgaa
     1201 tattgagtgg tcaaccttta tggcttctat gctggtcggc accattggta ttcaatggtc
     1261 gcgctggtat ctggcgcatc cgaaagtgtt taccgtggcg gccgttatcc ctatgttccc
     1321 gggcatatcg gcttataccg caatgatttc ggcggtaaaa atcagccagt taggttacag
     1381 cgaaccgttg atgattaccc tgttaaccaa ctttcttaca gcttcatcga ttgttggtgc
     1441 gttatccatc ggtctttcca ttcctggatt atggttgtac cgcaagcgcc ctcgcgtata
     1501 aaattgcctc gcttaagcac acggatgaga gacagcctcc tctcctccgt gtgttactat
     1561 aaaagttatc tcccttctcg ttcatcgttc catatttgag aaacagtatg tcttccagag
     1621 ttttgacccc ggacgtcgtt ggtattgacg ccctggtaca cgatcaccaa accgttctgg
     1681 caaaagctga aggcggtgtg gttgccgtat ttgctaacaa tgccccggcg ttttatgccg
     1741 tcacgcctgc acgcctggct gaactgctgg cgctggaaga aaagctggcg cgtccgggaa
     1801 gcgatgtcgc tctggacgat caactctatc aggaaccgca agccgctccc gttgctgtcc
     1861 ccatggggaa attcgccatg tatccggact ggcaacccga tgccgacttt atccgcctgg
     1921 cggcgctatg gggcgtggcg ctaagagagc cggtgaccac cgaagaactg gcctcattca
     1981 ttgcctactg gcaggcggaa ggtaaagtct ttcaccatgt gcagtggcaa caaaaactgg
     2041 cgcgcagcct gcaaatcggt cgtgccagca acggcggact gccgaaacga gatgtgaata
     2101 cggtcagcga acctgacagc caaattccac caggattcag agggtaacga tgaaaaacgt
     2161 tggcgacctg atgcaacgcc tgcaaaaaat gatgcctgcc catatcaaac ctgcattcaa
     2221 aacgggtgaa gaactactgg cctggcagaa agaacaagga gcgatccgct ccgccgctct
     2281 cgaacgtgaa aatcgggcga tgaaaatgca gcgcaccttt aaccgctccg gtattcgtcc
     2341 actgcatcag aactgctcct ttgagaacta tcgcgttgag tgtgaagggc agatgaatgc
     2401 gttaagcaaa gcgcgccagt atgtcgaaga gtttgacggc aacatcgcca gcttcatctt
     2461 ttctggtaag ccaggaaccg gcaaaaacca tctggcggcg gcaatctgca acgagctgct
     2521 gctacgcggt aaatccgtat tgatcatcac cgtggccgat attatgtcgg cgatgaaaga
     2581 taccttcagg aatagcggta ccagcgaaga acaactgctt aacgatctga gcaacgttga
     2641 tctgctggtg atcgatgaga tcggcgtgca gaccgaatcg aaatacgaaa aagtgatcat
     2701 caaccagatc gtcgatcgcc gctcttcttc caaacgccca accgggatgc tgaccaacag
     2761 taatatggaa gagatgacca agttgctggg cgaacgcgtg atggaccgta tgcgcctggg
     2821 taacagtttg tgggtgatct tcaactggga tagctaccgt agccgtgtaa caggtaaaga
     2881 gtattaatcc ccgtttctgg cggtatactg cgacacaatt ccaccttgag taatgatgat
     2941 gaaaactgtt aaacatcttc tgtgctgtgc cattgccgcc agcgcattaa tttccaccgg
     3001 ggtgcatgct gcgtcctgga aagatgcgct ctccagcgcc gccagcgaac ttggcaacca
     3061 aaacagtacg acacaggaag gcggttggtc gctcgcgtca ttaactaact tgcttagcag
     3121 cggaaaccag gccttaagcg cagataacat gaacaatgcc gcaggcattc tgcaatactg
     3181 cgcgaagcaa aagctggctt cggtaaccga tgccgaaaac atcaagaacc agatgctgga
     3241 aaagctgggc ctgaacagtg aagagcaaaa agaagacacc aactatctgg acggcattca
     3301 gggtttgctg aaaaccaaag atggtcagca actcaatctg gataacatcg gaacgactcc
     3361 gctggcagaa aaggtgaaaa ccaaagcctg cgatctggtg ttaaagcagg ggctgaactt
     3421 catttcctga accgcgaaac gccgcgtttt agctgttacc cggctgacgc ggctctctcc
     3481 tgcccttttc cctgctcctg gacggttttc cccctgttac acgggaagcg accgcgatca
     3541 aaaaagtctg tttataaact aattcctaac aacatcgcat ttttttgaca ctagaattgc
     3601 agcaatatgg cgacgccatt acattgtgct tctcaaaaag cttaaacgtt ggctaaacgg
     3661 cccggtttag tgaggatcgc ccgttgtcag aactactctc tttcgccctt tttctcgcct
     3721 ctgtgctgat ttacgcatgg aaagcgggac gtaacacctg gtggtttgca gccacattaa
     3781 cggtgctggg gctatttgtc gttttaaata tcaccctgtt tgccagcgac tattttactg
     3841 gcgatggtat taacgacgcg gttctctata ccttaaccaa cagcctgacc ggtgctggcg
     3901 tcagcaaata catcctgccg ggtatcggca ttgtgctggg gctgacagcg gtgttcggtg
     3961 cgctgggctg gatcctgcgc cgtcgtcgcc atcatccgca ccattttggt tacagcctgc
     4021 tggcgctctt actggcgctg ggttcggtgg acgccagccc agcatttcgt cagataacgg
     4081 aactggtgaa atcccagtca cgcgatggcg acccggactt tgcggcttat tataaagagc
     4141 cgtcgaaaac tatccctgac ccgaaactca acctggttta tatctacggc gaaagtctcg
     4201 agcggaccta ttttgataac gaagctttcc cggatctcac gcctgaactg ggcgcgttga
     4261 aaaatgaagg cctggatttc agccacacgc agcagctgcc aggaacggat tacacgattg
     4321 cgggcatggt ggcttctcag tgcggcatac cgctgtttgc cccctttgaa ggcaacgcct
     4381 ccgcctctgt ctccagcttc ttcccgcaga acatctgtct gggcgatatc ctgaaaaact
     4441 cgggttatca gaactatttc gtgcagggcg cgaatctgcg ttttgccggt aaagatgtgt
     4501 tcctgaagtc gcatggcttc gaccacttat acggctcaga agagctgaaa agcgtggtgg
     4561 ccgacccgca ctatcgcaac gactggggat tctacgacga taccgttctc gatgaagcgt
     4621 ggaaaaagtt tgaagagctt tcccgctcag gtcagcgatt ctcactgttt accctgacag
     4681 tcgataccca tcacccggat ggttttatct cgcgtacctg taaccgcaaa aaatatgatt
     4741 ttgacggtaa gccgaaccag tcattcagcg cggtaagttg cagccaggag aatatcgcgg
     4801 cgtttatcaa caaaatcaaa gcgtcaccgt ggtttaaaga taccgtcatc gtcgtctctt
     4861 ctgaccattt agcgatgaac aacacggcgt ggaaatacct taataagcag gatcgcaata
     4921 acctgttttt tgtcattcgt ggcgacaagc cgcagcaaga gacgctggca gtgaagcgta
     4981 acacgatgga taacggcgcg acggtgctgg acattctcgg tggcgataac tatctcggac
     5041 ttggtcgtag cagtttatcc gggcagtcaa tgtcggaaat cttcctcaat atcaaagaga
     5101 aaacattggc gtggaagccg gatatcatcc gcctgtggaa attccctaaa gagatgaaag
     5161 agttcaccat cgaccagcag aaaaacatga ttgccttctc gggtagccat ttccgtttgc
     5221 cgctgctgtt gcgggtttca gacaaacgcg tggaaccgct gccggaaagc gaatactcag
     5281 caccgctgcg tttccagctg gccgatttcg ctccacgcga caatttcgtc tgggttgacc
     5341 gttgctataa gatggcacaa ctctgggctc cggaactggc actctccacc gactggtgtg
     5401 tctcgcaagg gcagcttggc ggtcagcaaa ttgttcagca tgttgacaaa acaacatggc
     5461 agggcaaaac ggcatttaaa gatacggtca tcgacatggc gcgttataaa ggcaatgtcg
     5521 atacgctgaa aattgttgat aacgatattc gctacaaagc cgacagtttc atcttcaacg
     5581 tcgccggtgc gccagaagag gtgaaacagt ttagtgggat ttcccgtcca gagtcgtggg
     5641 gccgctggtc caacgcgcag ctgggcgatg aagtaaaaat cgagtacaag catccgctgc
     5701 cgaagaaatt tgacctggtg attaccgcca aagcatacgg caataacgcc agccgtccta
     5761 ttccggtacg cgtaggcaat gaagaacaaa ctcttgtgct gggcaatgaa gtgaccacca
     5821 ccacactgca tttcgataac ccaaccgatg ccgacacact ggtaattgtg ccgccggaac
     5881 ctgtctcaac caacgaaggg aatatcctcg gacactcgcc gcgtaagctc gggatcggca
     5941 tggtggaaat taaagttgtg gaacgtgaag ggtaatttct gactgatgtg taggtcgcat
     6001 aaggtgcgac ctacacatct tctccggcct acaaaacatc attgagaaac agaaaaaaaa
     6061 cagcgcccaa cacttccccg gacgctgcat ggcaacttta ataatctcgc cttatcagaa
     6121 agtaattaca cccttaatca actcacgatt gttaatcaca tcgcgctcgt aggtttctgc
     6181 cagcgtggcg aactgatagc gatgggttaa catcatgtcg gcagtgattt ttccttccgc
     6241 catcagtcga ccgactttag caaaatcttc cggcgtggcg ttgcggctgc ccatcatcgt
     6301 cgtttctttt ttatggaatt ccggatcgga gaactgcaac tcacctttaa acaggccgac
     6361 aaataccacc gtgccgccgt gacgaatcaa attcacggtg ttattcatcg catgttgatt
     6421 acctgtcgcg tcgatcactt tctgcgccag cgaaccacca aactgcgccc gtagctgcgc
     6481 gtcaaaatct tcggctgacg ggtccagtaa aggtaattcc agacgcgttg ccacatgttc
     6541 acggcgcgcc ggactggtat ccgccaccac cacctgtgcg ccatcggctt tagcgattgc
     6601 cgccgcgccc aaaccgattg gccccgcccc gaccaccagc acctgctcgc cgggagcaat
     6661 ggctgcgcga cgcaccgcat gagcgctaat agcgaaaggt tcaatcaatg cggccgcctg
     6721 cgggtcaata ccgtctgcgg gcaaaatgtt cgccaccggc acgctcaaat actcactaaa
     6781 accgccatcc tgatgcacgc caatgactga aattttttca cagcaattgg tacgcccgct
     6841 tttacacgcc gggcattgct gacaggcaac ataagggatc acagcaactt gctgaccatt
     6901 tttaagatca gcaatatttt tacccagccc aacaatctcc ccacatattt catggcctaa
     6961 aacacgtgga taactaaaaa atggttgatt tccaccccag gcatgaatat cggtaccgca
     7021 aatcccgaca gacttaattt ttattaatgc ttcattgtca cccggaatag gtatctcgcg
     7081 ttgtttccag actaattctt tcggctgctg gcaaattaaa acattcatcg tagacatgat
     7141 tcggctctcc attttttgtt gccttagata tcaatgaaaa tgaaaaatgg cagcggaacg
     7201 gaaaatctgt tttgtgaaaa cacacgcata aaaatgtttt aaatcgggtt ttaatggact
     7261 cacaaggagg aaaatatgag tcgttcacaa aatttacgcc acaatgtgat taaccaggtc
     7321 attgatgata tggcgcgggg tcatatccct tccccacttc catcgcaaag tgcgctggcg
     7381 gaaatgtaca acatcagccg cacaacagtg cgtcatattc tcagccactt acgcgaatgc
     7441 ggcgtcttaa cgcaggttgg aaacgactat gttattgcgc gtaaacctga ccatgatgac
     7501 ggtttcgcct gtactaccgc ttcaatgagt gaacaaaaca aagtgtttga gcaggctttt
     7561 ttcacgatga taaaccagcg tcagttacgc cctggggaaa ctttttctga actgcaactg
     7621 gctcgggccg cgggtgtcag cccggtggtt gtaagagaat atcttttaaa attcggccgt
     7681 tataatctta ttcaaagcga aaaacgcggc cagtggagca tgaagcaatt cgatcaatcc
     7741 tacgccgagc agctgtttga gctacgcgaa atgctcgaaa cacattcact tcagcacttt
     7801 ctcaacctgc ctgatcatga tccccgctgg ctgcaggcga aaaccatgct cgaacgtcat
     7861 cggctactgc gtgacaacat cggcaatagc tttcgcatgt tctcgcaact tgacagagat
     7921 tttcactcct tactgctttc agctgccgac aatatctttt ttgatcaatc gcttgagatc
     7981 atctcagtca tctttcattt tcactatcaa tgggacgaaa gcgatcttaa acagcgcaat
     8041 atcattgccg tcgatgagca tatgaccatt ctcagcgctc tgatttgccg cagtgacctt
     8101 gatgccaccc ttgcgctccg taaccatttg aattcagcta aacaatccat gattcgatcc
     8161 attaatgaga acacgcgtta tgctcattaa gtacggatta aaaacagcaa actattagcg
     8221 ggtgcacggt tttatcaaaa gataaatacc gggaaattcc cgcttaccta tgctcacaat
     8281 cagtcgacta aaaatttttt tgtgagcggt agtaaagtcc taaaacttta acctgccgtt
     8341 aatgtcaacc ttgcgtaatt cacaattacg taaacaatta aaattataaa tacaggagtc
     8401 cggcgtggaa aaagaaaata tcaccctcga tccgcgttct tcatttactc catcttcgtc
     8461 ggcagatatt cccgtgccac cagatggatt agttcaacgc agtacccgaa ttaaacgcat
     8521 tcaaaccacc gccatgttgt tattattttt tgcggcggta atcaattatc tcgaccgcag
     8581 ttcgctgtcg gtagcaaatt taacgattcg tgaagaattg ggattaagtg ccaccgaaat
     8641 cggcgctttg ctctccgtgt tttcactcgc ttacgggatt gcgcaacttc cttgcggccc
     8701 actattggat cgtaaaggcc cacgcctgat gctgggactg gggatgttct tctggtcact
     8761 gttccaggca atgtctggca tggtgcacaa ctttacgcag ttcgtgttgg tgcgtatcgg
     8821 tatggggatt ggtgaagcgc cgatgaaccc atgcggtgta aaagtcatta acgactggtt
     8881 caacatcaaa gagcgcggac gcccgatggg cttcttcaac gcagcttcta ccattggcgt
     8941 tgccgtaagc ccaccgattc tggcggcgat gatgctggtg atgggctggc gcgggatgtt
     9001 tattaccatt ggtgtactgg ggatttttct cgccatcggc tggtatatgc tctatcgcaa
     9061 ccgcgagcac gtagaactga ctgccgttga acaagcttat ctcaatgcag gtagcgtcaa
     9121 tgcccgccga gatccgctca gttttgccga atggcgcagc ctgttccgta accgtacaat
     9181 gtggggaatg atgctcggat tcagtggcat caactacact gcgtggctgt atctggcctg
     9241 gcttcctggt tacctgcaaa cagcctataa cctggattta aaaagcacag ggttgatggc
     9301 ggctatccct ttcctgtttg gggctgccgg gatgctggtc aacggttacg ttactgactg
     9361 gctggtcaaa gggggaatgg ctccgattaa aagccgtaag atctgcatta ttgccgggat
     9421 gttctgttct gccgccttta cgctgatagt accacaagcg acaacatcca tgacggcggt
     9481 tctgctgatt ggcatggcac tgttctgtat tcactttgcc ggaacatcct gctggggctt
     9541 gatccacgtc gcagttgctt ctcgcatgac tgcgtcggtg ggcagtatcc agaactttgc
     9601 cagcttcatc tgcgcctctt ttgcgccgat cattactggt tttattgttg ataccaccca
     9661 ctcattccgt ctggcactaa tcatctgcgg ttgcgtcacc gcagcggggg cactggcgta
     9721 catcttcctg gttcgtcagc cgatcaacga cccacgtaaa gattaatctc cttatgcccg
     9781 ataacatttt gcttatcggg cattttcatg gcgattaaaa tgtttcccag ttctcctcgc
     9841 tatctgccac ggccatttta cgcggcgcag ctggcgtcac ggtttttacc acagccgatg
     9901 tttcacgctg ctgttgctgc tgttgctgaa tccggaacac tgccacagct tcggtcaggc
     9961 gactggcctg ctcttccagc gcggcggcgg cagcggcaga ttcttccacc agcgcggcgt
    10021 tctgttgagt tacccggtcc atctcagcaa ccgctaagcc aacctgatcg ataccacggc
    10081 tctgctcatc agaagcagaa gcaatttcgc ccataatgtc cgtcacgcgg gtcacggcgc
    10141 tgacaatctc cgccattgtt tccccggcgc tttcgaccag cgtagagcca acatccactt
    10201 tccccaccga gtcttcaatc aggcttttaa tttcacgagc cgcctgagcg ctacgctggg
    10261 ccagattacg cacttctccc gcaaccaccg caaaaccgcg cccttgctca cccgcacgcg
    10321 ctgcttcaac cgccgcgttc aaagccagaa tattggtctg gaaggcaatg ccgtcaatta
    10381 cgctgataat atcggcgatt ttctgcgaac tggtggagat atcgcgcata gtctgcacca
    10441 cgttatctac cactttgccg ccccgttgcg ccgtttcaga agcacttaat gccagatggc
    10501 tggcctggcg cgcattctcg gcgttctgtt tcaccgttgc ggtcagttgc tccatgctgg
    10561 ctgccgtctc ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga tcgttattgc
    10621 cggtagcgat ttcgctggca ccgctataga tggcattggc cccgttgcgc acatcaccga
    10681 cggtacgcat cagctctccc tgcatatggc gcaaactctc tgccagttgc cccatctcat
    10741 tagagccatc cacctcaatc ggtttcacca gatcgccgcc tgcaatatga cgaatgctgt
    10801 caatcaggcg attcattggc gctaccagcg aggctttaat accgaaccag acggcgaaga
    10861 tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg ctgtaggagg
    10921 cattgttatc gctgacggcg atatcataga gccgatcgtt ttgctccatg taagccacat
    10981 actgcttctc gaaaccgtcc tgatatccct gagtcggctg atcaaagaac tcgttgattt
    11041 tgcctgcacc taacagttgg atcagctccg ccagcgcatt gtgataaata tcgtaattac
    11101 gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac gcttcgtaat
    11161 ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc agctcagcaa
    11221 cggttgaacc gctaccaata ttattctgat ccatcatgta gcggataccc gcgcggttga
    11281 gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtggat tgctgctggc
    11341 gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta aagaacagac
    11401 cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag ctggtcacga
    11461 tcttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat tttcggcctg
    11521 gaaagcaaaa actttatgaa aaggtgtaac ccgccatcca tggaaaaacc aggcgctggt
    11581 tatcagatac taaaaagtta ttcattatcg cccgggatgt cttcttgtcg ggaattgccc
    11641 agagcaataa attcctctaa caaatgggtt aactgttgca ttttctcggc agtgaactga
    11701 gcttcaatct gccgataggc ttcttcaatc tgtgtctgag cgcggttata cagcgcctgc
    11761 ccctctttgg tcagcgagat atagagcttc cgctgatcat tgatcggctt taatcgcaac
    11821 actaaaccgt cgcgttccat ccgcgtaagg atcccggtca ggcttgggcg caaaatgcag
    11881 gcgcgatacg ccagatcgtg aaaatccatt gatgggctct ccgccagaat acgcacgatg
    11941 cgccactgtt gctcagtcaa attatgccgt ttaacgattg ggcgaaaata actcatcgcc
    12001 gcttctcgcg cctggagcaa cgcaatggtt agagagtcgt gcattatctt tcccctgaaa
    12061 aattcaaaat cattaatata gaaacagtta atcatcacgt taccgggact gcgctgcccc
    12121 tgcaattctt cattttatca aaattgagca tgttttgtta acttgctgat atttaatatt
    12181 ttattatctt
//
LOCUS       DAZFXT010000130        11958 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.130, whole genome shotgun sequence.
ACCESSION   DAZFXT010000130 DAZFXT010000000
VERSION     DAZFXT010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.130"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..1134
                     /gene="phoE"
                     /locus_tag="ACHKW0_003960"
     CDS             79..1134
                     /gene="phoE"
                     /locus_tag="ACHKW0_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HFO7476710.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            complement(1173..1574)
                     /gene="crl"
                     /locus_tag="ACHKW0_003961"
     CDS             complement(1173..1574)
                     /gene="crl"
                     /locus_tag="ACHKW0_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045893 - positive regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HFO7476711.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(1632..2876)
                     /gene="frsA"
                     /locus_tag="ACHKW0_003962"
     CDS             complement(1632..2876)
                     /gene="frsA"
                     /locus_tag="ACHKW0_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7476712.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            complement(2968..3426)
                     /gene="gpt"
                     /locus_tag="ACHKW0_003963"
     CDS             complement(2968..3426)
                     /gene="gpt"
                     /locus_tag="ACHKW0_003963"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7476713.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3687..5144
                     /gene="pepD"
                     /locus_tag="ACHKW0_003964"
     CDS             3687..5144
                     /gene="pepD"
                     /locus_tag="ACHKW0_003964"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7476714.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            complement(5201..5815)
                     /gene="prfH"
                     /locus_tag="ACHKW0_003965"
     CDS             complement(5201..5815)
                     /gene="prfH"
                     /locus_tag="ACHKW0_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO7476715.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALISLDGDNAWALSESWCGAIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5812..6951)
                     /locus_tag="ACHKW0_003966"
     CDS             complement(5812..6951)
                     /locus_tag="ACHKW0_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HFO7476716.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            complement(7197..7649)
                     /gene="yafP"
                     /locus_tag="ACHKW0_003967"
     CDS             complement(7197..7649)
                     /gene="yafP"
                     /locus_tag="ACHKW0_003967"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7476717.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            complement(7646..8701)
                     /gene="dinB"
                     /locus_tag="ACHKW0_003968"
     CDS             complement(7646..8701)
                     /gene="dinB"
                     /locus_tag="ACHKW0_003968"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HFO7476718.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(8772..9557)
                     /gene="lafU"
                     /locus_tag="ACHKW0_003969"
     CDS             complement(8772..9557)
                     /gene="lafU"
                     /locus_tag="ACHKW0_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HFO7476719.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPKSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            9529..11241
                     /locus_tag="ACHKW0_003970"
                     /pseudo
     CDS             9529..11241
                     /locus_tag="ACHKW0_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140831.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0044780 - bacterial-type flagellum assembly
                     [Evidence IEA];
                     GO_process: GO:0071978 - bacterial-type
                     flagellum-dependent swarming motility [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            11346..11624
                     /locus_tag="ACHKW0_003971"
     CDS             11346..11624
                     /locus_tag="ACHKW0_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HFO7476720.1"
                     /translation="MIKVSALGSRHRKTFDDVMTEPPKSGIKWDDVVALIKAVGGTIK
                     NNNGSRRKFQIGTTKFSTHEPHPKNVMDKGAVAGLKEWFVNCVGVDYE"
     gene            11617..>11958
                     /locus_tag="ACHKW0_003972"
     CDS             11617..>11958
                     /locus_tag="ACHKW0_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HFO7476721.1"
                     /translation="MNKASNIITIKGQPAAVTFEADINAFRGKFLNVNGYCDFVATSI
                     EALYREGESALDEWMADCEEDGIAPFREEEEQKRLTLRVPHHIDSRLTIVARQHSISK
                     NQLIVDVLEREF"
BASE COUNT         2976 a         3088 c         3018 g         2876 t
ORIGIN      
        1 ctcaggttaa aaactttcct gttttcaacg gggctctccc gctgaatatt cgcgcgttaa
       61 ttaaaatcag gaatgaaaat gaaaaagagc actctggcat tagtggtgat gggcattgtg
      121 gcatctgcat ccgtacaggc cgcagaaata tataacaaag acggtaataa actggatgtc
      181 tatggcaaag ttaaagccat gcattatatg agtgataacg acagtaaaga tggcgaccag
      241 agttatatcc gttttggttt taaaggcgaa acacaaatta acgatcaact gactggctat
      301 ggccgttggg aagcggagtt tgccggaaat aaagcggaga gtgatactgc acagcaaaaa
      361 acgcgtctcg cttttgccgg attgaagtat aaagatttgg gttctttcga ctatggccgt
      421 aacctgggcg cgttgtatga cgtggaagcc tggaccgata tgttcccgga atttggtggc
      481 gactcctcgg cgcagaccga caactttatg accaaacgcg ccagcggtct ggcgacgtat
      541 cggaacaccg acttcttcgg cgttatcgat ggcctgaact taaccctgca atatcaaggg
      601 aaaaacgaaa accgcgacgt taaaaagcaa aacggcgatg gcttcggcac gtcattgaca
      661 tatgactttg gcggcagcga tttcgccatt agtggtgcct ataccaactc agatcgcacc
      721 aacgagcaga acctgcaaag ccgtggcact ggcaagcgtg cagaagcatg ggcaacaggt
      781 ctgaaatacg atgccaataa tatttatctg gcaacttttt attctgaaac acgcaaaatg
      841 acgccaataa ctggcggctt tgccaataag acacagaact ttgaagcggt cgctcaatac
      901 cagtttgact ttggtctgcg tccatcgctg ggttatgtct tatcgaaagg gaaagatatt
      961 gaaggtatcg gtgatgaaga tctggtcaat tatatcgatg tcggggctac atattatttc
     1021 aacaaaaata tgtcagcgtt tgttgattat aaaatcaacc aactggatag cgataacaaa
     1081 ttgaatatta ataatgatga tattgtcgcg gttggcatga cctatcagtt ttaatgatta
     1141 ttgccggatg tgatgcatcc ggcacatttc actcacgccg ttaacttcac cggctcgtca
     1201 cgaaaatcat ccgccggttc cagcttcaga ttcagcgtcg tcagcagttc acgcagcttc
     1261 tcgtgaaact cacgcagggt gtgctccagt cgttcaacca cttcagtgtc ttttaccgga
     1321 acgctcttcc agtcgcctgc tttatcgaac agaccaaact ggtagctgta ggtaaaacgg
     1381 gattcctgcg cttccagctc catccaccag ccccagaatt cacgcacttc cggtgccggt
     1441 ttcacgttga cgcatacagc cagacaatcg aaaaagaatc gattatcttc gcacttacct
     1501 tcacgaatat acgggcctag tgcggtaaat tttttgatca atctgctctt cgggtgtcca
     1561 ctcggtaacg tcattgctat ctcctgttgt gatgcaactg ttttaccaaa ttggcaaaat
     1621 ttagcaaatt tttaacacaa gcgtttttcg atccagccgg tgatttcctg aagacctttg
     1681 tcaaaattcc gatacaccgg gttaaatggg atctctaata atttaccgtc agcagatgat
     1741 gaggtgatta agcgtgagtc ctcttccggg ctgaacggat cgttcttcca gtagcctgat
     1801 aacattggcg ttgggcagcg acgtcccagc aatccttgca cttttaatga atagcgattc
     1861 agctccacgc gcaacgcgtc atcggaagca tcatgcatcc ccaaacgact cgccagaacg
     1921 tcaagataca tttccggcac ctgttgctgg cacttaaaat cactcaacag ggtatgaact
     1981 accggaccaa gacaggcaac cgctttcaga cgcggcgatt caaggtatgc cagacgcacg
     2041 gcaacgttag cgccgaaacg gaaaccaaag gccgcgacgc gagtgtgatc cacccacggt
     2101 acgttaggca gcgcctttaa gacgtgctga tgcaacaggc tggagtcctg ggtgagcttc
     2161 cattttgaag aaaagcccac cgacggcata tcaatagtca gcatcgcaat gccgcgcggc
     2221 gcaaaataac gttcatacag gctgtaatag tccgtctgca tcgcatccag accaccacac
     2281 attaatactg tcgggaacgg gccatcgcct ttcggcatat gcaaaaagcc ggtgatgggc
     2341 gcaccgccgg gtacggtaaa ctccatctgc cgcatcgtgc ccggcagacg ctgggcggcc
     2401 tcttcatagg cgcggtttga taaagcctgc gcttgctcgg ccaggtcatc tcctttcaga
     2461 tgaggatagg cggcaatgtt gtacaacgta gccgcatgca gccagtgtcg accactgagt
     2521 tgcggatctt cttccgcaca ggctttttgt tgccacacca tcgcctgggt ggcccactca
     2581 taaatccagt tgccaccacg gtagccaatc acagtatcgt ataaatcatc gtcggtacgt
     2641 tcagcatcgc tcatcacaat acgtgcctgg acgtcgagga tttcacgtgg gtcaataccg
     2701 cgccagatcc acatcagacg gttaatcatg cgataccagt gagggatggt tttaccatca
     2761 agagccgact gcacaggcgg ttgtgcgccg tgattaaagc ggcggactag cgtcgaggtt
     2821 tcaggatgtt taaagcgggg tttgaacagg gtttcgctca ggtttgcctg tgtcatggat
     2881 gcagcctcca gaatacttac tggaaactat tgtaacccgc ctgaagttaa aaagaacaac
     2941 gcccggcagt gccaggcgtt gaaaagatta gcgaccggag attggcggga cgaatacgac
     3001 gcccatatcc cacggctgtt cgatccaggt atcttgcggg atatcaacaa cataatcatc
     3061 aaccagcggg cgaccggccg gttttgcgaa gatggtgaca aagtgcgctt ttggatacat
     3121 ttcacgaatc gcaaccgcag taccaccggt atccaccagg tcatcaataa cgatgaagcc
     3181 ttcgccatcg ccttctgcac gtttcagcac tttaagctcg cgctggttgt cgtgatcgta
     3241 gctggaaata caaacggtat cgacatgacg aatacccagt tcacgcgcca gtaacgcacc
     3301 cggtaccaga ccgccacggc ttacggcaat aatgcctttc cattgttcag aaggcatcag
     3361 tcggcttgcg agtttacgtg catggatctg caacatgtcc caggtgacga tgtatttttc
     3421 gctcatgtga agtgtcccag cctgtttatc tacggcttaa aaagtgttcg aggggaaaat
     3481 aggttgcgcg agattataga gatctggcgc actaaaaacc agtatttcac atgagtccgc
     3541 gtctttttac gcactgcctc tccctgacgc gggataaagt ggtattctca aacatatctc
     3601 gcaagcctgt cttgtgttga caacattttc tgctaaccct gtgacctgca atactgtttt
     3661 gcgggtgatc gacaaggaga cttaacgtgt ctgaactgtc tcaattatct ccacagccgc
     3721 tgtgggatat ttttgccaaa atctgttcta ttcctcaccc gtcctatcat gaagagcaac
     3781 tcgctgaata cattgttggt tgggcaaaag agaaaggttt ccatgtcgaa cgcgatcagg
     3841 taggtaatat cctgattcgt aaacctgcta ccgcaggtat ggaaaatcgt aaaccggtcg
     3901 tcttacaggc ccacctcgat atggtgccgc agaaaaataa cgacaccgtg catgacttca
     3961 cgaaagatcc tatccagcct tatattgatg gcgaatgggt taaagcgcgc ggcaccacgc
     4021 tgggtgcgga taacggcatt ggtatggcct ctgcactggc agttctggct gacgaaaacg
     4081 tggttcacgg cccgctggaa gtgctgctga ccatgaccga agaagccggt atggacggtg
     4141 cgttcggctt acagagcaac tggttgcagg ctgatattct gattaacacc gactccgaag
     4201 aagaaggtga aatctacatg ggttgtgcgg ggggtatcga cttcacctcc aacctgcatt
     4261 tagatcgtga agcggttcca gctggttttg aaaccttcaa gttaacctta aaaggtctga
     4321 aagggggtca ctccggcggg gaaatccacg ttggcctggg taatgccaac aaactgctgg
     4381 tgcgcttcct ggcgggtcat gcagaagaac tggatctgcg ccttatcgat ttcaacggcg
     4441 gcacactgcg taacgccatc ccgcgtgaag cctttgcgac cattgctgtc gcagctgata
     4501 aagtcgacgt cctgaaatct ctgatgaata cctatcagga gatcctgaaa aacgagctgg
     4561 cagagaaaga gaaaaatctg gccttgttgc tggactctgt agcgaacgat aaagctgccc
     4621 tgattgcgaa atctcgcgat acctttattc gtctgctgaa cgccaccccg aacggtgtga
     4681 tccgcaactc agacgtggca aaaggtgtgg tcgaaacctc cctgaacgtc ggtgtggtga
     4741 ccatgactga taataacgta gaaattcact gtctgatccg ttcactgatc gacagcggta
     4801 aagactacgt cgtgagcatg ctggattcgc tgggtaaact ggctggcgcg aaaaccgaag
     4861 cgaaaggcgc atatcctggc tggcagccgg acgctaattc tccggtgatg catctggtac
     4921 gtgaaaccta tcagcgtctg ttcaacaaaa caccgaacat ccagattatc cacgcgggcc
     4981 tggaatgtgg tctgttcaaa aaaccgtatc cggaaatgga catggtttct atcgggccaa
     5041 ctatcaccgg tccacactct ccggatgagc aagttcacat cgaaagcgta ggtcattact
     5101 ggacgctgct gactgaactg ctgaaagaaa ttccggcgaa gtaattattt gatttgctgc
     5161 cggatggcgt ttaatcgcct tccggcagtt tcatccttca ttatccttcg ataaaagcca
     5221 tccctgtaaa tgtccgtctc gggttgccac gttcaatctg gtgatggaac attcgccgct
     5281 gcgatttcag caccgcgcta ttttcctgtt gctgctgctc cagcttccag gcaatcagca
     5341 atcgtgccag ccgcttgtta gcatgctgac tacgctctga ctgaaccttc acgctaatac
     5401 cggatgccaa atgcgtggcg cgtaccgccg agtcggtttt attgacatgt tgaccgcccg
     5461 gccccgacga acgcaacgtc tcataacgga ttgcatccga ttgttcctgc tcatcagcgg
     5521 taaaacgccc aatgcccaga aaccagtttt tgcgcccatg atgaggccga tacggactcg
     5581 gacaaatcca ctgaatagcg ccacaccagc tttcgcttaa tgcccatgcg ttatcgccat
     5641 ccagagaaat cagcgccgaa cgcagcgtgt cagagtagcg gcccgtttct gtttccaaca
     5701 ccgttaccgc gacgtcttgt cgggcagctt ctttaataag cctgtccagc gcctttttca
     5761 ctgcgagaca acattcttcc ggcccctgcg ctgaggagag ttgtagcaag atcatccttt
     5821 tttccctcca ctgtttttga gcgttagcac cggacgcagc cgcgcgacag gaataattaa
     5881 accggcctgc accagacatt gcaccacgct ttcagccgat ttataggctt gtggcgcttc
     5941 ttcaaagatg agttgtttat cgcgacaaat tacccgactg ccaagttcag tacgcgagag
     6001 ttgcgtcgcc gtgtatttcg ctgccagacg ccctttacac tcggtgcgcc cccatttacg
     6061 ccctgcccca tgcgccagcg agtggagcgt tttttcgttc gcaacaggct gtacaatcca
     6121 ggagtaatca ccacgtgaac cgggaataat caccagaccg ttgtcatccg gcgtggcacc
     6181 tttacgatgc aaccagccct gttgatcgcc gatttgacac gcgctaacaa agttatgcgc
     6241 cacatccaga accggacttc ccgtggcctt aacttgttgc attatgcgca aagcgatcat
     6301 ctggcgatta atacacgcaa acgccagcgc atcgtcatgt tccgcaatat accgtagcgc
     6361 gtcgtcactg ccttgaggca aaccatgatg cgagaacgag gcaatatgcc gctgtaaaat
     6421 agactgcccc accccccgcg agccgctatg aaccaacagt tgcagatgtt gtgcatccag
     6481 acctgccagt gcaaacagtt cagcgttgat gatttgatca acttgttgca gttcagcgaa
     6541 atgattgccg ccaccgatag agccaagcga gctgcgccac ggatgctgtg caaatgctga
     6601 cggcaggttt tcctccagcc agctttcttc agcaacgtca tccagatctg ataatcgctt
     6661 ttcaaactta tcggcgttgt atttgcgagc gagaatatct gtttgccata gcgccatacc
     6721 gcagccgata tcattgccga ccagtgccgg ataaaaacga ccaacagaga agaacgctgc
     6781 gccaatcgga tagccgcgtc cgggatgtaa atctggcatc cccactacgc gctgcatgtt
     6841 gggtaaattt gccgtggtgt gtaattgttg gatcgctaaa ctctcgatcc acaggtcatc
     6901 agatgcgatg gtaaataccg catcggataa gggacgaata taattgccca tataccattc
     6961 cgtaaatgga ctcgtcatac ttcaagttgc atgtgttgcg acttgaatta ttgagagtaa
     7021 caattaaaaa atcaggaaat ggcagacgaa tccagggcaa gaaaaaaaga attaatcctg
     7081 gaaaggtctg caaagagtca ttgataaagg atttgtcatg atttacctcc ttgcagtttg
     7141 tggaataaga aaacgcgcac atactaggct tgcgttattt tcattgcaag ctggatttaa
     7201 tgttgctgtt tataacgcat ataaaaatta ataaaccact ctccccgaca ttcaacgcgt
     7261 tgctgcttaa ctgtctgaaa accataacgt tcaaaaaagg gttttgcggt tatgcttgcg
     7321 tccaccgtaa gttcggattc agacttaatc aaaggtttta acaaagcgct ggcaacccca
     7381 cggcgggtgt attcagggtc aacaaataac atatcgatat agtgttcaac gcaggtaata
     7441 aagccgacca gttttgcatt aatgactgca acccgcactt gtgatttcgc gagtttctcc
     7501 ttccagcgag attcgtcaat ctgcgcccag gcggcaattt gttgtggtga ataatgctga
     7561 ctggcagtca tcgtaaccgc tctaatgaaa atagcgcata gttgctgaaa atcgccaggc
     7621 tgatagtctc ttatttgtat gttattcata atcccagcac cagttgtctt tccatttgcg
     7681 ggtcaagcaa cgtcacatgc agccccacca gtcgcacacc gcgcccgccg cggcgttcat
     7741 cccaggtttt acgcgcggtg gcgattaagt cagctttatt cagccgcggc cagacgtgct
     7801 cctgagtggt ttgctgaaaa tcgtcgaact ttaatttcac cccctggcgg gcaatcagta
     7861 aatcaggttt tacctttgcc agacggcgtt caagttccgg atacagccgc tcgataatcg
     7921 cttcacattc agaccagtgg tggatatctt ccgccatcgt gcgttccacg ccgacggatt
     7981 ttcgcaaccg ttcgctgtta acgtcgcgct cgtcaatccc ctgactacgc tcccacaaaa
     8041 tgcggccaaa tttgccaaag cgtttaagca gcatcaccag atcacacttt tgtacatcac
     8101 cgcaggtccg cagccccatc gcttccagtt ttgccgccga gactttgccg acgccgggga
     8161 tttttgccag cggtaaggtt tgtaaaaatg ccggaacttc tgccggcgta atcacaaact
     8221 gaccgttggg tttattcatg tcggaggcga ttttggcgag aaactttact ggtgccacgc
     8281 ccgcagacgc tgtcagttgc agctcgctga aaatcgtctg gcggatttcc tgggcgatga
     8341 gggtcgcaga accgtggcaa tggacgctat cggtgacgtc gagataagcc tcatccagtg
     8401 acaacggttc aatacgcgag gtgtagcgcg agaagatttc acggatatga tttgaggctt
     8461 ctttgtaggc gtcaaagcgc cccggaagca aggtgagatg cgggcataat ttgagcgcca
     8521 tccctgtcgg catagcgcta cgtacgccaa atttacgcgc gggataattg gcggtgctga
     8581 tcaccccccg acgttcgcgg ctgccgccaa tagcaatagg gatatcgcgc agggcgggat
     8641 tgtcgcgcat ctccaccgct gcgaaaaagc agtccatatc cacatgaatg attttacgca
     8701 ttactcacct ctcaacactg gtaaagtata cagtgatttc agggtttgag aaatgcgtaa
     8761 agattcagca tttaacgcgt tcgctgcgaa agcacctgct gcttatccag cttttgcacc
     8821 agcggctgca ccactttatc cccatgctga ccaaagtatt gatacagcgt atcggacgca
     8881 cttttggtca gcaccataat ctcaatgcgc cggttgcccg cgcttttcgg atttttggaa
     8941 tccagcagca tctggtccgc cattgcgctt acctgcatca ctttatcttc cggcattccg
     9001 gcctcttcca gcacccgacg agccgaaagc gcgcggtcac ccgaaaggtt ccagttgttg
     9061 tagatattgt ttttgtaagc catcgcatcg gtatgcccgg taataataat tttgttatcg
     9121 agcgagtcga aaactggcgc gagctccacc agcagcgttt taaagaacgg cataatctgc
     9181 gcgctgccgc gttcgaacat attgcgattc tggtcgtctt taatcagcac gcgtaatccc
     9241 tggggaacaa tctccatctc cagattcgct tccatatgcg catcacgggc aatggtggtg
     9301 atactggttg ccagctcgcc aagctcggtg gcggatttct tcttcagcag cgccgttttc
     9361 tcattgacgt cgcgcgcttt tttctccgtt tcttccggca ccgccacggt ggcaggtttt
     9421 ggcggatgag acgggctgat tttatttaac ggcgacagcc caccaccgtt aaaaatcgac
     9481 tgcccgtgca gcgcggcgat aatgctttca cgctcggatt tactgacgct gttgacaatc
     9541 aactttattg tcgtcaccaa aggggccgag cgtatctccg aggtttctgc ccgctttacc
     9601 ctggacgcga tgcccggtaa gcagatggcg attgacgccg atcttaatgc cgggttgatc
     9661 aatcaggcgc aggcacaaac ccgacgtaaa gatgttgcca gcgaggccga tttctacggc
     9721 gcgatggacg gggcatcgaa atttgtgcgc ggggatgcta tcgccggtat gatgattctg
     9781 gcgatcaacc tgatcggcgg cgtctgtatc gggatcttca aatacaacct gagcgccgac
     9841 gccgccttcc agcagtatgt gctgatgacc atcggcgacg ggctggtggc gcaaatccct
     9901 tccctgctac tctccaccgc ggcggcgatt atcgtcaccc gcgtcagcga caacggcgat
     9961 atcgcccatg acgtgcgtaa ccagttgctg gcaagcccat cggtgctcta caccgctacc
    10021 ggaattatgt tcgtactggc ggtagtgccg ggaatgccac atctgccgtt tttgctgttc
    10081 agcgccctgc ttggctttac cggctggcgg atgagcaaac gcccgcaggc ggcggaagcg
    10141 gaagagaaaa gtctcgaaac gctgacccgc accatcaccg aaaccagcga gcaacaggtc
    10201 agttgggaaa ccattccgct gatcgagccc atcagcttaa gcctcggcta caagctggtg
    10261 gcgctggtgg acaaagccca gggcaacccg ctcacccagc gtattcgcgg cgtgcgacag
    10321 gtgatatccg acggcaacgg cgtgctgctg ccggagatcc gcattcggga aaacttccgc
    10381 cttaagccca gccagtacgc tattttcatc aacggcatta aggctgatga agcggatatt
    10441 ccggcggata aactgatggc gctgccctcc agcgaaacct acggcgagat agacggcgtg
    10501 ctggggaacg acccggcgta cgggatgccg gtgacctgga tccagcctgc gcagaaagcg
    10561 aaggcgctga atatggggta tcaggtgatc gacagcgcca gcgtgattgc tacccatgta
    10621 aataagattg tgcgcagcta tattcctgat ttatttaact atgacgatat tacgcagttg
    10681 cataaccgtt tggcgtcgat ggcaccgcgc ctggcggagg atttaagcgc ggtgctcaat
    10741 tacagccagt tgctgaaagt gtaccgggcg ctgctgaccg aaggcgtttc cctgcgcgat
    10801 atcgtcacca tcgccaccgt gctggtcgcc agtagcgcgg tgaccaaaga tcatattctg
    10861 ctggcagccg atgtgcgcct ggcgctgcgg cgcagcatta cccatccgtt cgttcgcaag
    10921 caggagctga cggtgtatac cctgaataat gagctggaaa atctgctgac taacctagtg
    10981 aatcaggcgc aacagggcgg taaagtgatg ctcgacagcg tgccagtgga cccgaatatg
    11041 ctcaaccagt tccagagcac gatgccacag gtgaaagagc agatgaaagc ggcggggaaa
    11101 gacccggtgc tgctggtgcc accgcagctg cgccctttgc tggcgcgtta tgcaaggttg
    11161 tttgcgccgg ggctgcatgt gctgtcgtat aacgaagtgc cggatgagct ggagttgaag
    11221 attatggggg cgttgagttg atgggggatt cttttccatg ctttgcagca cctgacacca
    11281 ttaattgacg tcacactgca aagagactac gatgacacca tacaatgacg tcatggattg
    11341 atagtatgat taaggtcagc gcgctaggtt ctcgtcatag aaaaacattc gacgatgtaa
    11401 tgacggagcc gccaaaatct ggaattaaat gggatgatgt tgttgcactc attaaagctg
    11461 tgggtggaac aataaaaaat aacaacggct ccagacgaaa atttcagatt ggaacgacca
    11521 aattcagcac acatgagcca catccaaaga atgtgatgga taaaggcgca gtagcaggtc
    11581 ttaaggagtg gtttgttaac tgtgtagggg tagattatga ataaagcatc gaacattatc
    11641 acaattaaag gtcaaccagc cgcagtgaca tttgaagccg atatcaacgc atttcgcggc
    11701 aagttcctca atgtaaacgg ttattgtgac tttgtagcca caagtattga ggcattatat
    11761 cgtgaaggcg aaagcgcgct ggatgagtgg atggcggatt gtgaagagga cggtattgcc
    11821 ccttttcgtg aagaagaaga gcaaaaacgc ctgacgcttc gtgttcctca tcacattgat
    11881 tcgcggctga ccatcgtcgc cagacaacat tccatttcaa aaaaccagct cattgttgat
    11941 gtcttagaac gtgagttt
//
LOCUS       DAZFXT010000131        11913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.131, whole genome shotgun sequence.
ACCESSION   DAZFXT010000131 DAZFXT010000000
VERSION     DAZFXT010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.131"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..315)
                     /gene="yehE"
                     /locus_tag="ACHKW0_003973"
     CDS             complement(34..315)
                     /gene="yehE"
                     /locus_tag="ACHKW0_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HFO7476722.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(578..1687)
                     /gene="apbC"
                     /locus_tag="ACHKW0_003974"
     CDS             complement(578..1687)
                     /gene="apbC"
                     /locus_tag="ACHKW0_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HFO7476723.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIVSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPGLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1819..3852
                     /gene="metG"
                     /locus_tag="ACHKW0_003975"
     CDS             1819..3852
                     /gene="metG"
                     /locus_tag="ACHKW0_003975"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HFO7476724.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3993..7787
                     /locus_tag="ACHKW0_003976"
     CDS             3993..7787
                     /locus_tag="ACHKW0_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HFO7476725.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAVAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEKYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7797..11429
                     /gene="yehI"
                     /locus_tag="ACHKW0_003977"
     CDS             7797..11429
                     /gene="yehI"
                     /locus_tag="ACHKW0_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein YehI"
                     /protein_id="HFO7476726.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPAQTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLVVTLQYQNEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESCWQRCADKLIATLPGITKVRRPFIALILPEKPEIANELVSL
                     ECPRTHFHSKEWLKVVANDPTAVRKLERYWSQDIFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARLGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRLIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11490..11810
                     /gene="yehK"
                     /locus_tag="ACHKW0_003978"
     CDS             11490..11810
                     /gene="yehK"
                     /locus_tag="ACHKW0_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YehK"
                     /protein_id="HFO7476727.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
BASE COUNT         3113 a         3040 c         2934 g         2826 t
ORIGIN      
        1 ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta acacgattcg
       61 acgtttgcta tcgctacctg caacaaccac ttctgtcgtt acacctttga cgggcgaaga
      121 aaacagatga cgggtgtcaa cattatacgt taactgacca caatgctgtt gaggttgacc
      181 attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg ctaatgtggc
      241 agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa ttcctgacag
      301 ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga tagaatgagt
      361 gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata atgaagcatt
      421 gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa tattcgcagt
      481 attaaataag tgttcattaa tgcggcaaaa actaatacac cgcatcaatg taacatctct
      541 gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg aaggaaatct
      601 cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca gccagctggc
      661 ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg cctttatcga
      721 gatcctcacg taaggagatg tgtaacggca tctgccccag cagctgtgtg tggtattttt
      781 cagccagttt ctcagcacca ccggtaccga aaatcggttc atggtgacca cagttactgc
      841 aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact ttctcgaaca
      901 tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg accacgactg
      961 caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg cctggcggca
     1021 tatcaagcac cagatagtcg agacccggcc acaaggtttc ctgcaacatc tgcattaacg
     1081 ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgacc agataaccaa
     1141 ttgaattggt tgccaggcca tgagacacaa tcggtgccat atgggtaccg tccggcgaag
     1201 ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag atatcggcgt
     1261 ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt accgccgtgg
     1321 aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc acaccattaa
     1381 tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt ttccagtcga
     1441 tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt aactcttcaa
     1501 acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg tccatccagg
     1561 cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg tgctgaaaat
     1621 tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc tgggattgtt
     1681 cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt acctgaagaa
     1741 tatagagaag tacttactta acattttccc atttggtact atctaacccc ttttcactat
     1801 taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac gtgcgcactg
     1861 ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca ggctgatgtc
     1921 tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc cgacgatgcc
     1981 cacggtacac cgatcatgct gaaagctcag cagctcggta tcaccccgga gcagatgatt
     2041 ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag ctatgacaac
     2101 tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta ctctcgcctg
     2161 aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc ggaaaaaggc
     2221 atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc cccggatcaa
     2281 tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt gatcgagccg
     2341 aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt cttctttgat
     2401 ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt gcaggagcag
     2461 gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga tatctcccgc
     2521 gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt ctacgtctgg
     2581 ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa gcgcggcgac
     2641 agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta ccacttcatc
     2701 ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga aggcagcaac
     2761 ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg cgcaaagatg
     2821 tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt tgacgctgac
     2881 agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat cgatctcaac
     2941 ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt taacctggcc
     3001 tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag cgaattggct
     3061 gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga agcgtgggaa
     3121 agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct ggctaaccgc
     3181 tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga tgccgacctg
     3241 caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta cctgaagccg
     3301 gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact gacctgggat
     3361 ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct gtataaccgc
     3421 atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt gaaagcagct
     3481 gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat cacctttgac
     3541 gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt tgttgaaggt
     3601 tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa tgtcttctcc
     3661 ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac cattatggtg
     3721 gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt aatggctgct
     3781 ggtcctggcg ggaaagatat cttcctgtta agcccggatg ctggtgctaa accaggccat
     3841 caggtgaaat aatccccctt taaggcgctg catcgacagc gccttttctt tataaattcc
     3901 taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg gtagcatctg
     3961 cattcaactg gataaaatta cagggatgca gaatgagaca ctttatctat caggacgaaa
     4021 aatcacataa attctgggca gttgagcaac agggaaacga gttgcatatc agttggggaa
     4081 aagttggcac caaagggcaa agccagataa aaagtttttc agatgctgcg gcagtggcaa
     4141 aagcggagct taagctgatt gcggagaagg tgaagaaggg gtatgtggag caagcgaagg
     4201 ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg gacttatcca
     4261 ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac aaaaatacag
     4321 atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc gaagttgtac
     4381 acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca gacaaattga
     4441 cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca gccacatttg
     4501 acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc agtcagatcg
     4561 acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg gcacttgaaa
     4621 tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc caggaaggcg
     4681 gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt aaatgggatt
     4741 atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac ttggagcaat
     4801 attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag tctctctggc
     4861 aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatgg cggcaaccat
     4921 taattgcttt gttattaccc gaaaaaccag aaattgcaca tgaaattgcc cagcgtctac
     4981 tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact gatgagcaca
     5041 ttcttgcctc attagaaaaa tatcacgagc catatgccat ttttgatgat tactattgtg
     5101 gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg ccccgatttg
     5161 ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat catccgttcg
     5221 cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat cggatgacga
     5281 aagcctgcgc tgcgttccca catgcagcaa tggcggcact gacggaactt cttgggcaaa
     5341 aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa ccagcactgg
     5401 cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa tcatgccagc
     5461 aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc gtagtagtct
     5521 cccctccctg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat ttagcgccat
     5581 taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt ttggcgaaat
     5641 atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc aacctgttgg
     5701 caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc gaagcggtag
     5761 ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag gtgtttcatt
     5821 cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg gagaaagcag
     5881 taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac gccatgttac
     5941 attttggact caaagggtta cctgggttag taaactcact tgcacgctat ccacaagaag
     6001 ccttgcccat cacgaattac ttcgcagcga gtgagctggc tcctgccgtc gcccgtgcct
     6061 tcaacaaact gaaaacgcta cgcgaaaacg cccgtagctg gctgttgaaa tacccggaac
     6121 atgcccttac cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca caggataacg
     6181 cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca gccattactg caagaaatcg
     6241 cccgacgtta taaccagccg gaagtaaccg atgcggtgaa cgctctgctt gcgctcgatc
     6301 ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag ccatcgctct
     6361 ggacgcgccc ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc ctcctccacc
     6421 tcggtgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta ttgcaggtga
     6481 aagacgtctg ttccgccgac tcactggcgg gatttgcctg ggatctgttt accgcctggc
     6541 agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc gttctcggta
     6601 acgatgacac cgcccgcaaa ctgacgccat taatacgcgc ctggcctggt gaatcccagc
     6661 ataaacgcgc caccgttggg ttggatattc tcgctgctat cggtagtgat atcgccctta
     6721 tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag cgggcaaaag
     6781 aaaaaattgc cgacattgcc gagagccgcg aactcacggt ggcggagctt gaagatcggt
     6841 tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttt ggcccacggc
     6901 agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt tccggcagcc
     6961 gcctgaaaga cctgcccaaa ccgaacaaaa gcgatgatga aacgcgggcg aacgatgcgg
     7021 ttaaccgcta caaattgctg aaaaaagatg cgcgtaccat cgccgcccag caggtagcaa
     7081 ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgct ggaaaacttc cagctcttcc
     7141 tggttgagca tccgctggtt cgtcacttaa cccgccgtct gatttggggc gtttatagcg
     7201 ccgaaaacca gctactggct tgctttcgcg tagcagaaga taacagctac agcaccgctg
     7261 acgatgatct tttcaccctg ccggaaggcg atatctctat cggcactcct cacgttctgg
     7321 aaatatcacc aacggatgct gccgcctttg gtcagctttt tgccgactac gaactgctac
     7381 caccgttccg ccagctcgac cgtaacagct acgccctgac agaagccgag cgcaatgcca
     7441 gtgaactgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg ggactggcga
     7501 ataaaggttg ggtgcgtggt gaaccgcagg atggtggctg gatcggctgg atgatcaaac
     7561 ctctgggccg ctggtcgtta atcatggaaa tcgatgaagg ctttgcagtt ggtatgtcgc
     7621 cagccgaact tagcgccgag caactcttaa gcaagctgtg gctatgggaa ggcaaagcag
     7681 aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta ctcgatgcca
     7741 tcaccgccag cgagctgatt aacgatattg aagccctgtt tgaataagga aagagcatgg
     7801 acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat aaaaaaggca
     7861 aaacaccact cagccatcgc aattggccgg gcgaaccagt gcctgttatc actgaaagca
     7921 tcattcagac actgggtgat gaattgctac aaaaagctga gaagaaaaaa aacattgtct
     7981 ggcgttatga gaatttttca ctggagtggc agtccgccat tacgcaggcc atcaacttga
     8041 tcggcgaaca caaaccctca atcccggccc agacaatggc ggcgctagtc tgtatcgcgc
     8101 aaaatgacag ccaacagttg ctcgacgaaa tcgtccaaca agaggggctg gaatatgcga
     8161 ctgaggtggt gattgcacgc cagtttatta cacgatgtta tgagagtgat cctctggtag
     8221 tcacattgca gtatcagaac gaggactatg gctatggtta tcgctcagaa acctataacg
     8281 aattcgatct ccgactgcgt aagcatctct ctctggcaga ggaaagctgc tggcagcgtt
     8341 gcgccgacaa actcattgcc acactgccag gaataaccaa agttcgccgc ccttttattg
     8401 cgctgatcct ccccgaaaaa ccagaaatcg ccaatgagtt ggtaagcctt gaatgcccgc
     8461 ggactcattt tcattctaag gagtggttaa aagttgttgc taatgacccc acagcggtga
     8521 gaaaactcga acgctactgg agccaggata tatttagcga tcgagaagcc agctacatgt
     8581 cgcatgaaaa ccacttcggc tacgcggcct gcgccgccct tttgcgcgaa caaggactgg
     8641 cagccgttcc gcgcctcgcg atatatgccc ataaagaaga ttgcggcagt ctgctggtac
     8701 aaattaacca tccgcaagtc atccgcacct tgctgttagt tgctgataaa aacaaaccca
     8761 gcctgcaacg tgtggctaaa tacagtaaaa acttccccca tgcgacactc gccgcactgg
     8821 cagaactgct ggcgttaaaa gaaccaccag cccgcctagg ttatccaatc atcgaagaca
     8881 aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg ttacaaacgc
     8941 tgatagcatc gcagccacaa ctagcagaaa gggtgatgcc gtggttaagt actcaagcca
     9001 gggcagtgct gaatagttat ttatcggcac cgccaaaacc ggttattgat agcaccgata
     9061 acagcagtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa aaaatgacag
     9121 ctccacgtct tgatttggca ccgctcgaat taactccgca aatttactgg caaccaggcg
     9181 aacaagagag gcttgccgcc actgagtctg cccgttattt cagcacggaa tctcttgcgg
     9241 aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt ggggatgatg
     9301 tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc aattcactca
     9361 ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc tggaactccc
     9421 cgcaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg ttaattaaca
     9481 tatgggaaaa tgacaacttc tcacgttatc gtccggaaaa gagtgtctgg aacctgtatt
     9541 tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc attgagaaaa
     9601 agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat gcgctaccag
     9661 gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt ttaaattttg
     9721 gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg gcgcagcgtg
     9781 atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca cttatccctc
     9841 ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg cgtttactgt
     9901 acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag cgtacagatg
     9961 tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac ccggcacgca
    10021 ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga cttatcacta
    10081 ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg cgctttaccc
    10141 aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag ccacaaacgc
    10201 tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc cccgcaaaag
    10261 acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca cgggatctga
    10321 cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc agcggcctga
    10381 acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat atatcgcaac
    10441 gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggtg gtcgcagaaa
    10501 atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg ggccttgatg
    10561 atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc ttcgatgaaa
    10621 acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagcgtt ccccggttgc
    10681 gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa gggctaaaaa
    10741 aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc ctacgtacca
    10801 cccgacgctg gtcgctggca gattttcata ctctgtttgt taatcatccc tttacccgcc
    10861 tggttaccca gcgattaata tggggcgtgt atccggcaaa tgaaccgcgt cgtttactca
    10921 acgcctttcg tgtggccgca gagggggagt tctgcaatgc gcaggatgag ccaattgacc
    10981 tgcctgcgga tgctctgatt ggcattgccc acccgttaga aatgacagca gaaatgcgca
    11041 gtgaatttgc ccagcttttt gccgattacg aaattatacc gccacttcgc cagttgacac
    11101 ggcgcacggt gctgctcacg cctgacgagt cagccagtaa tagtttgaat cgctgggaag
    11161 gtaaatctgc taccgttggg caacttatgg gaatgcgata caaaggctgg gagtcaggct
    11221 atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag ttttcacccg
    11281 gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct cttcgagtgt
    11341 acagtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg agtgaggcgt
    11401 taagcgcacc tgacgtcatt ttccattaaa acacagcggg cagtgatgca actgcccgtt
    11461 atcaacacga cttaccagcg gggaaagcga tgatcgtgca aaaagagctg gttgctattt
    11521 acgattatga ggtccctgta cctgaagatc cgttttcctt cagacttgag atccataaat
    11581 gctctgaatt atttacaggt tccgtctatc gactggagcg attccggcta cgtccaacat
    11641 ttcatcaacg tgatcgagaa gatgctgacc cgctaataaa tgatgcgttg atttatataa
    11701 gagatgagtg tattgatgag cggaaattac gaggtgaatc acctgaaact gtaatagcaa
    11761 tttttaatcg tgaactacag aatatattca accaactaat agaatattag tttattaaag
    11821 ggggaagcgg ttttccccct tagttgcgtt tcaatttgcc cattacttca acaaatcaat
    11881 caaccgtaac agagacacta atagctccag tac
//
LOCUS       DAZFXT010000132        11851 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.132, whole genome shotgun sequence.
ACCESSION   DAZFXT010000132 DAZFXT010000000
VERSION     DAZFXT010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.132"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..558
                     /locus_tag="ACHKW0_003979"
     CDS             <1..558
                     /locus_tag="ACHKW0_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476728.1"
                     /translation="KFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCSIV
                     PDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSANSG
                     NYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNFKA
                     WLVGAADAPDLGNFEANTTFQITYL"
     gene            640..3282
                     /gene="yfcU"
                     /locus_tag="ACHKW0_003980"
     CDS             640..3282
                     /gene="yfcU"
                     /locus_tag="ACHKW0_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HFO7476729.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVANNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3302..4054
                     /locus_tag="ACHKW0_003981"
     CDS             3302..4054
                     /locus_tag="ACHKW0_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HFO7476730.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKSNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4029..4577
                     /locus_tag="ACHKW0_003982"
     CDS             4029..4577
                     /locus_tag="ACHKW0_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476731.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4574..5044
                     /locus_tag="ACHKW0_003983"
     CDS             4574..5044
                     /locus_tag="ACHKW0_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476732.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5041..5565
                     /locus_tag="ACHKW0_003984"
     CDS             5041..5565
                     /locus_tag="ACHKW0_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7476733.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5552..6424
                     /locus_tag="ACHKW0_003985"
     CDS             5552..6424
                     /locus_tag="ACHKW0_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001397279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="StfH/YfcO family fimbrial adhesin"
                     /protein_id="HFO7476734.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6546..7097)
                     /gene="smrB"
                     /locus_tag="ACHKW0_003986"
     CDS             complement(6546..7097)
                     /gene="smrB"
                     /locus_tag="ACHKW0_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HFO7476735.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7263..8195
                     /gene="prmB"
                     /locus_tag="ACHKW0_003987"
     CDS             7263..8195
                     /gene="prmB"
                     /locus_tag="ACHKW0_003987"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HFO7476736.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            8230..9315
                     /gene="aroC"
                     /locus_tag="ACHKW0_003988"
     CDS             8230..9315
                     /gene="aroC"
                     /locus_tag="ACHKW0_003988"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HFO7476737.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9319..10143
                     /gene="mepA"
                     /locus_tag="ACHKW0_003989"
     CDS             9319..10143
                     /gene="mepA"
                     /locus_tag="ACHKW0_003989"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HFO7476738.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFAPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10143..10952
                     /locus_tag="ACHKW0_003990"
     CDS             10143..10952
                     /locus_tag="ACHKW0_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HFO7476739.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10952..11500
                     /gene="epmC"
                     /locus_tag="ACHKW0_003991"
     CDS             10952..11500
                     /gene="epmC"
                     /locus_tag="ACHKW0_003991"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HFO7476740.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11534..11812
                     /locus_tag="ACHKW0_003992"
     CDS             11534..11812
                     /locus_tag="ACHKW0_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HFO7476741.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         2933 a         2890 c         3095 g         2933 t
ORIGIN      
        1 aagtttgtta aaacagctat tgctgcggca atggtgatgg gcgttttcac ctctacggca
       61 acaatcgctg cgggtaacaa cggtactgca cgtttttacg gcaccattga agactcagtg
      121 tgttctatcg ttccggacga tcacaaactg gaagttgata tgggtgatat cggtgccgaa
      181 aaactgaaaa ataacggcac caccacgccg aaaagtttcc agattcgtct gcaagattgc
      241 gtatttgata ctcaggaaac aatgaccact acctttactg gtaccgtttc ttctgcaaat
      301 agcggcaatt attacaccat tttcaatacc gataccggtg cggcatttaa caatgttagc
      361 ctggcgatcg gtgactctct gggtacctct tacaaaagcg gcatgggtat tgaccagaaa
      421 atagtgaaag acacttctac caataaaggc aaagcgaagc agacactgaa ctttaaagcc
      481 tggctggtcg gcgcagctga tgcgccagat ctgggtaatt tcgaagcaaa taccaccttc
      541 cagattactt acctgtaagg cataccccca tagcgacact gctatggggg atttaaatgg
      601 attttgaccg aacaagaata aaaacggata gaaacgtgta tgcctgacca ttctcttttt
      661 cgattacgga ttcttccgtg gtgcattgcg ctggcaatgt cagggagtta tagcagtgtc
      721 tgggctgaag acgacattca gtttgattcc cgttttctgg aattaaaagg cgacacaaaa
      781 attgatctga agcgtttttc cagccaggga tatgttgagc ccggaaaata caatttacag
      841 gttcaactaa ataaacagcc attggcggaa gagtacgata tttactggta tgctggtgaa
      901 gatgacgcga gcaaaagcta tgcttgtctg acaccggaac tggtagcgca gtttggttta
      961 aaagaagacg tggcgaacaa tctgcaatgg agccacgatg ctaaatgcct gaaatccggt
     1021 caactggaag gcatggaaat taaggctgat ttaagccagt ccgcattagt catttcactg
     1081 ccacaggctt acctcgaata tacttatccc gactgggatc cgccttcacg ttgggatgac
     1141 ggcatctccg ggatcgtcgc ggactacagc atcaacgcac aaacccggca cgaagaaaat
     1201 ggcggtgatg atagtaacga gatcagcggc aacgggacgg tcggggttaa cctggggccg
     1261 tggcgtatgc gtgctgactg gcagactaac tatcaacata ctcgcagtaa tgatgacgat
     1321 gaattcagcg gcgatgaaac tcaaaaaaaa tgggagtgga gtcgctacta tgcctggcgg
     1381 gcgttaccat cattaaaagc caaactggcg ctgggcgagg attacctcag atccgatatt
     1441 tttgatggtt ttaactatgt tggtggcagt gtcagtactg acgatcaaat gttgcctccc
     1501 aatctgcgcg gctacgcgcc agacatttcc ggcgtggcac acaccacagc aaaagtgacc
     1561 gtcagccaga tggggcgtgt gatttacgaa acgcaggttc cggctggacc gtttcgtatt
     1621 caggatcttg gtgattctgt ctccggtacg ttgcatattc gcattgaaga acagaacggc
     1681 caggtgcagg aatatgacat cagcaccgcc tcgatgccat acctcactcg cccaggtcag
     1741 gttcgctata agatcatgat gggccgtccg caagagtggg gacaccatgt cgagggtgaa
     1801 tttttttctg gtgctgaagc ttcctggggg atcgctaacg gctggtcgtt atatggcggc
     1861 gcactgggag atgaaaacta tcagtctgcg gcgcttggcg tcggtcgcga tttgtctaca
     1921 ttcggcgcgg tcgcgtttga tgttactcac tcgcacacca aactggataa agacaccgct
     1981 tatggcaaag gttcgctgga cggtaactcc ttccgtgtga gttattccaa agactttgac
     2041 cagctcaaca gccgcgttac ttttgctgga tatcgcttct cggaagagaa ctttatgacc
     2101 atgagcgagt atctggatgc cagtgacagc ggaatggtac gcacgggcaa cgacaaagag
     2161 atgtacaccg ccacttataa ccagaacttc cgcgatgcgg gtgtttcggt ttatctcaac
     2221 tatacccgcc atacctactg ggatcgcgag gagcagacaa actacaacat catgctctcg
     2281 cactatttca atatgggcag tattcgtaat gtcagcatct cgatgactgg ctaccgttac
     2341 gagtatgaca accaggccga caaaggcatg tacatttcgc tcagtatgcc gtggggcgac
     2401 aacagtaccg ttagctataa cggtaactat ggcagtggga cggacagcag tcaggtcggt
     2461 tatttcagcc gtgtcgatga cgcgactcac tatcagttga acgtcggcac cagtgacaaa
     2521 cacaccagcg ttgacggcta ttacagccat gatggttcgc tggcgcaggt tgacctcagt
     2581 gcgaactacc atgaagggca atacacctct gcgggcttgt cgttacaggg cggcgcaacg
     2641 cttactgccc acggtggcgc acttcaccgt acccagaata tgggcgggac acgcttgttg
     2701 attgatgccg atggcgttgc cgatgttccg gtagaaggta acggtgctgc tgtttatacc
     2761 aatatgtttg gtaaagccgt cgtttctgac gtcaataact attaccgcaa tcaggcgtat
     2821 atcgacctca acaaactgcc ggaaaacgcc gaagcaaccc agtcggtggt acaagccacg
     2881 ctaactgaag gggcgattgg ctaccgcaaa tttgccgtca tcagtggtca aaaagcgatg
     2941 gcggtgctgc gtttacaaga cggcagccat cctccgtttg gcgcagaagt aaaaaatgat
     3001 aacgagcaga cagtgggcct tgtcgatgat gacggcaatg tttatctggc tggggtgaaa
     3061 cctggcgaac acatgagtgt gttctggagt ggtgttgcgc attgcgatat caacctgccg
     3121 gacccgctgc ctgccgatct gtttaacggc ttgttactgc catgccagca taaaggcaac
     3181 gtcgcgccgg ttgttcctga tgacataaag cctgtcattc aggagcagac gcaacaggtg
     3241 acacccacca atccccctgt ttctgtttca gctaaccaat aacgtgatta aggaatgatc
     3301 catgtctgat ttactttgct ctgccaaatt gggtgctacg acgctagcgt tactgcttag
     3361 cgctgccagt ttgtctgccc aggcttctgt aacgccagat cgtacccgtt tgatatttaa
     3421 tgaaagcgac aaatcaatca gcgtgacgtt gcgcaataac gatccgaaac tgccttatct
     3481 ggcccaaagc tggatcgaag atgaaaaggg taacaagatc agttcaccgt taactgttct
     3541 gccaccggtg caacgcatcg attcaatgat gaacgggcag gtaaaagtgc agggaatgcc
     3601 ggatatcaac aaattgcctg ccgatcgtga aagcctgttc tacttcaacg tgcgtgagat
     3661 cccgccaaaa tcaaacaaag ccaacacgct gcaaatagca ctacaaacgc gtattaagtt
     3721 gttctggcga ccgaaggcgc tggagaacgt cagcatgaaa aacccctggc aatataaggt
     3781 tactttaacc cgcaacggtc aggaatttac cgtcaataac ccgacaccgt attacgtcat
     3841 tttcagtaat gccagcacac agaaaaatgg caatccggca gccggtttct cgccaatagt
     3901 gatgtcgccg aaaacctctg caccgctgaa tgtgaagatg ggtagcgtgc cggtactgac
     3961 ctatgtcaac gactatggcg ctcggatgcc gctctttttc acttgcaacg gtaacagttg
     4021 ccaggtcgat gaagagcaga gccgcaaagg ctgaggccgg gagagccagt atgaaaggat
     4081 cttgttctgt ctgggcggta actgggttac tgatatgttg tgttggtagc ggctttgcag
     4141 ttgcagcgag taagtcagta agtatgaatt tacgggtgct tgtcgatgcc cctcctcctt
     4201 gtacggtaaa tggtgcggca gttgagtttg gtaatgtatt cattaataag atcaatgggg
     4261 ttgattataa acgtcccatt gactactcgc ttgtttgtaa caatctggca atggatgacc
     4321 tgcgtctaca gatgcaggcg acaacggtcg tgattaacgg tgaaacagtt atcagcacgg
     4381 ggattcctgg gtttggtatt cgggttcaga aaagtagtga tcacacaata ctcgacttaa
     4441 cctctggctc atggttgcca tttaattttt cctcgggagt tcccgttctg gaagctgtgc
     4501 cggtgaaaca gagtggtaca acgttagcgg cagctgaatt taacgccagt gccacaattg
     4561 tggtggatta ccaatgaaac ggatctcgct tattttgctg tggggctttt gctcgatggc
     4621 actctcaaac gtctcttttc acggttattt agtgcagcca cctaactgca cgattagcaa
     4681 cgctcaaacc attgaaataa cttttcagga tgtgcttatt gatgacatta atggcagcaa
     4741 ttatgagcaa accgtgccgt attccattac ctgcgatacg gcggttcgcg acccattaat
     4801 ggaaatgacc ttgagctgga gtggcacacc aagcgatttt gataatgctg cagtaagcag
     4861 caatatcact ggtttaggca ttcagctcaa acaggcaggg cagagtttta cgatcaatac
     4921 gccacttgtg gtgaatgaaa ccgatttacc ggtgttaacg gctgtgccag taaaaaagag
     4981 tggtgtcgtt ctgccagagg cggactttga ggcctgggcc acgttacagg tagattacca
     5041 atgaagaaaa agcgtacgtt gttttttatt agtagcctga tgttgctggg cagtggaacc
     5101 acaatagcag gagataattt gcactttacc ggcaatttaa tcagtaaatc gtgtacgcca
     5161 gtgattaatg gtagccagtt ggcagaagta cattttcctg ccattgccgc cagcgattta
     5221 atgaaccttg ggcagtcaga gcgagtgcca ttggtgtttc aattgaaaga ttgtcatagt
     5281 tctacattgt ttaatgtcaa agtaacgttg acaggaacag aagatagtgc tctacccggc
     5341 tttctggcct ttgactcctc gtcatcagca tccggcgctg ggattggtat tgaaactgcg
     5401 gcaggcacat cggtgccaat caacaataca acaggcgtca cgctcccgct gaatcaggga
     5461 aataacagcc taaattttaa tacctggtta caggcaaaga gcgggcgaga tgtaacgtcc
     5521 ggagacttta gtgccacagt gacggcaacc tttgagtatt tctgaaatga aaaaatggac
     5581 cataattttc gcgagcctaa tgctgctcgt tctgagtgtt gtcggcgcat ctaagagtta
     5641 tgctggagat aaacttatga gtgcgagttt tgacagtgtg agagtttatt atgccatgga
     5701 caaggtaacc ggtgctgtag cgagcagtgt tttcaatgtt acggtgataa cgccaaagga
     5761 ggttgcatat gggaaatatg attcttttgc gtataaaggc gatacgttgc gggttatttc
     5821 ctggtctggg tcgggttcag ctccaacttt ggtactgact gactttgata caattaataa
     5881 ctcaaattgc cctggcatag atacgaaaat ttttcgttgt gcatatatga cgtttaaaat
     5941 taccgttgca tctgatgatt atggttgccc ttggatcgca tccttttatt cctatacaga
     6001 tttacccgga tttggaagct atacggcccc tacagtgcat aacacaatat gcccaacgat
     6061 accggtcgct tcatacgata tctcctggag tgaaaattat gtcagccata ataaggcgtt
     6121 acgaattcag tccacggggt caacagtgac caccacgctt tcgacctact tgatggaagg
     6181 cggcagactc tgtgatggca gtaatttttc tgataatgat ggcagaggag catactgccg
     6241 cgcggtatca gaattgttaa cctttacctc ttacggttgt gataaatcaa cggtaacggt
     6301 tacgccaact cgtcatccgg taacggataa agtactacac gatattgtgg tgaatgtgaa
     6361 taccagtagt gggcaaccga ttgattcgac gtgtcgtttt cagtatgtat tgaatgaact
     6421 gtagttcgta gtattgctct gatgcgtctt atcaggccta cgaagtgaat tgcaatttat
     6481 tgaattatca catatttgta ggccggataa ggcgtttacg ctgctccggc atttgctgcc
     6541 agcattcagg gcaactccgg cggcaaccac tcttccactt caatcaacac caacaaagcc
     6601 gcatcaccgc cgtactcttt cggtgcctgg tgaaatgcca tcacatgcgg atgttgtgcc
     6661 agccacagcg gtgtttgttg cttcaaaata tgcttcccgt gcccatgcat cacgcaggca
     6721 caaaacacat gttcgcggcg acaggcggca atcaacgcgc ccagttcctg cttggcctgc
     6781 agctgcgtca gaccgtgtaa atccaaaaac aattccggtg aataatcgcc gcggcgcagt
     6841 ttcttcgcct caaaatggct gacatccggg cgaacatatt tcaccggacc ttcggtattt
     6901 aataacggct gaaactcatc ggagaaataa tggctggcat cagcctgctc ctggatcaag
     6961 cgtttcaccg gcacttcgct gattttttta cgctgcggtc ggtggacaat cgtgtcctgc
     7021 ttaatcttgc gagtccccgc catcaactgg cgaaacagag cctggtcctc ctcgctaagt
     7081 gttgttttct ttttcattta tctgtctcta tttctaattt tcctacagtt tacccgactc
     7141 gtagcacaat cgatcgtttt aaacgtattt ttcctcactc accgcgcgtg aatgctgatt
     7201 tatcgtcatc ttcatggcaa actagccgcc gaaattattg cgatcatgcc ctggaggaat
     7261 acgtggataa aattttcgtt gatgaagcag taaatgagct gcaaaccatt caggacatgt
     7321 tgcgctggtc ggtaagccgc ttcagcgcgg caaatatctg gtacggtcac ggcaccgata
     7381 acccgtggga tgaagccgta cagctggtgt taccatcgct ctacctgccg ctggatatcc
     7441 cggaagatat gcgcaccgcg cgtctgacct ccagcgaaaa acaccgtatt gttgaacgcg
     7501 tgatccgccg cgtcaacgag cgcattccgg tggcttacct gaccaacaaa gcctggttct
     7561 gcggtcatga attttacgtc gatgaacgcg tgctggtgcc gcgctcaccg attggtgaac
     7621 tgatcaacaa taaatttgcc ggactcatca gcaagcaacc gcagcatatt ttagatatgt
     7681 gtaccggtag cggctgcatc gccattgcct gtgcttatgc cttcccggaa gcggaagtcg
     7741 acgcggtaga tatctctccc gacgcgctgg cggttgccga acagaacatc gaagaacacg
     7801 gtctgatcca caacgtcatt ccgattcgtt ccgatctgtt ccgtgatttg ccgaaagtgc
     7861 aatacgatct gattgtcact aacccgccgt atgtcgacgc ggaagatatg tccgacctgc
     7921 caaacgaata ccgtcacgag ccggaactgg gcctggcatc tggcaccgac ggcctgaaac
     7981 tgacgcgtcg cattctcggt aacgcggcag attaccttgc ggatgatggc gtgttgattt
     8041 gtgaagtggg caacagcatg gtacatctta tggaacaata tcccgatgtt ccgttcacct
     8101 ggctggagtt tgataacggc ggcgatggag tgtttatgct caccaaagag cagcttattg
     8161 ccgcacgaga acatttcgcg atttataaag attaagtaaa cacgcaaaca caacaataac
     8221 ggagccgtga tggctggaaa cacaattgga caactctttc gcgtaaccac cttcggcgaa
     8281 tcgcacgggc tggcgctcgg ctgcatcgtc gatggtgttc cgccaggcat tccgctgacg
     8341 gaagcggacc tgcaacatga ccttgatcgt cgtcgccctg ggacatcgcg ctataccacc
     8401 cagcgccgcg agccggatca ggtcaaaata ctctccggtg tttttgaagg cgttactact
     8461 ggcaccagca ttggcttgtt gatcgaaaat accgaccagc gttctcagga ttacagcgca
     8521 attaaagacg tcttccgtcc aggccatgct gattacacct acgaacaaaa atacggtctg
     8581 cgcgattatc gcggcggtgg acgttcttcc gcccgcgaaa ccgccatgcg cgtggcggca
     8641 ggggcgattg ccaaaaaata tctcgccgag aaatttggta ttgaaatccg cggctgcctg
     8701 acccagatgg gcgacattcc gctggaaatc aaagactggt cgctggttga acaaaatccg
     8761 ttcttctgcc cggaccccga caaaatcgac gcgttagatg aactgatgcg cgcgctgaaa
     8821 aaagagggcg actccatcgg cgcgaaagtc accgttgttg ccagtggtgt ccccgccgga
     8881 cttggcgagc cggtctttga ccgcctggat gccgacatcg cccatgcgct gatgagcatc
     8941 aacgcggtga aaggcgtgga aattggcgac ggttttgacg tagtggcgct gcgcggcagc
     9001 cagaaccgcg acgaaatcac caaagacggt ttccagagca accatgcggg cggcattctc
     9061 ggcggtatca gcagcgggca gcaaatcatt gcccatatgg cgctgaaacc gacctccagc
     9121 attaccgtgc cggggcgcac tattaaccgc tttggcgaag aagttgagat gatcaccaaa
     9181 ggccgtcacg atccctgtgt cgggatccgt gcagtgccga tcgcagaagc gatgctggcg
     9241 atcgttttaa tggatcacct gttacggcag cgggcgcaaa atgccgatgt gaagactgat
     9301 attccacgct ggtaaaaaat gaacaaaacc gcgattgcgc tgctggctct gcttgccagt
     9361 agcgctagcc tggcagcgac gccgtggcaa aaaataaccc aacctgtgcc gggtagcgca
     9421 caatcgatag gcagtttttc taatggctgt attgtcggcg ctgacacgtt gccgatacag
     9481 tccgaacatt atcaggtcat gcgtaccgat cagcgtcgct atttcggtca cccggatctg
     9541 gtgatgttta tccagcgtct gagtagccag gtgagcaatc tgggcatggg tacggtgctg
     9601 attggcgata tggggatgcc cgctggtggg cgtttcaacg gcggtcatgc cagccaccag
     9661 accggactgg atgtcgatat ctttctgcaa ctgccgaaaa ctcgctggac ctccgcgcag
     9721 ctcttgcgcc cgcaagcact ggacttagtt tcccgcgacg gtaaacacgt tgttccgacg
     9781 ctgtggaagc cagaaatttt cagcttgatc aaactcgccg cccaggacaa agacgtcaca
     9841 cgcatttttg ttaatccggc gattaaacaa caactttgcc ttgatgcggg caccgatcgc
     9901 gactggttgc gcaaagtgcg accctggttc cagcatcgcg cgcatatgca tgtacgatta
     9961 cgttgtcctg ccgatagtct ggagtgtgaa gatcaacctt taccgccacc aggcgatggt
    10021 tgcggggcag aactgcaaag ctggtttgca cctccaaaac cgggaacaac aaagcctgag
    10081 aagaagacac cgcctccgtt gccgccttcc tgccaggcgc tactggatga gcacgtgatc
    10141 taatggaaac gtttaatagc ctgtttatgg tttccccgct gttgctggga gttctctttt
    10201 ttgtcgccat gctggcggga tttatcgact cgattgccgg tggcggtggg ttactcacca
    10261 ttccggcatt gatggcagcg ggaatgtctc ccgctaacgc gttggcaacc aataaactgc
    10321 aagcctgcgg cggctctatt tccgccacta tctactttat tcgccgcaaa gtggttagtt
    10381 taagcgatca gaaactcaat attgccatga cctttgtcgg ctcaatgagc ggcgcactgt
    10441 tggtacagta cgttcaggct gatgtcttac ggcagatttt gcccattctg gtgatttgta
    10501 tcggcctcta tttcttgctg atgccaaagc tgggtgaaga agatcgccag cgccggatgt
    10561 acggtttacc cttcgcgtta attgccggtg gctgcgtcgg tttttatgac ggattctttg
    10621 gcccggctgc cggatcgttt tacgcgctgg ccttcgttac gctgtgcgga ttcaacctcg
    10681 ccaaagccac ggctcacgcc aaattactca acgcaacgtc aaacattggc ggtctgctgc
    10741 tgtttattct cggcggcaaa gtgatttggg cgacgggctt tgtgatgctg gtggggcagt
    10801 tcctcggcgc gcgcatgggt tcacggctgg tgttgagcaa aggacaaaag ctgatccgcc
    10861 cgatgattgt tatcgtctcg gcggtgatga gtgccaaatt actttacgat agccacggac
    10921 aggagatcct ccactggttg gggatgaact aatgaacagt acacaccact atgagcagtt
    10981 gattgaaatt tttaatagct gctttgccga tgaatttaat acccgtctga ttaaaggcga
    11041 cgacgaaccg atctatcttc ctgctgatgc ggaagtaccg tataaccgaa ttgtctttgc
    11101 ccacggcttt tatgccagcg caattcatga gatttcgcac tggtgtattg ccggaaaagc
    11161 gcgccgtgag ctggttgatt tcggctactg gtattgcccg gatgggcgtg atgcccaaac
    11221 gcaaagccag tttgaagatg ttgaagtgaa gccgcaggca ctcgactggt tgttctgtgt
    11281 ggcggcggga tatccgttta acgtcagttg cgacaatctg gaaggcgact tcgaaccgga
    11341 tcgtgtggtg tttcaacggc gggtacatgc gcaggtgatg gattacctag caaacggcat
    11401 tcctgaacgc ccggcacgct tcattaaagc actacaaaat tattatcaca cgccggaact
    11461 tacggcggaa cagttcccgt ggccggaagc gctcaactaa agccagcgcc acgttttaca
    11521 atgaggaaag aagatgatcg cggagtttga atcacgcatt ctggcattaa tcgacggtat
    11581 ggttgaccat gccagtgatg atgagttgtt tgccagtggg tatttgcgtg gccacctgac
    11641 attagccatc gcagaactgg aaagtggtga tgaccactcc gctcaggcgg tgcatacgac
    11701 cgttagccag agtctggaaa aagccattgg tgccggtgaa ttgtcgccgc gtgaccaggc
    11761 gctggtgacc gatatgtggg aaaacctgtt tcagcaggcg tcacagcagt aagttgcggc
    11821 gtctggagca ccatgcctga tgcgacgctt a
//
LOCUS       DAZFXT010000133        11744 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.133, whole genome shotgun sequence.
ACCESSION   DAZFXT010000133 DAZFXT010000000
VERSION     DAZFXT010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11744
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.133"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            8..196
                     /locus_tag="ACHKW0_003993"
     CDS             8..196
                     /locus_tag="ACHKW0_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476742.1"
                     /translation="MSHDGLFALAQKKMPDATLRVLYSHICQIQQRIRLPQLAHFHTC
                     PPYQKFILKLLNNHFPAN"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="ACHKW0_003994"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="ACHKW0_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HFO7476743.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /gene="yfiM"
                     /locus_tag="ACHKW0_003995"
     CDS             complement(1608..1931)
                     /gene="yfiM"
                     /locus_tag="ACHKW0_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HFO7476744.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHKW0_003996"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="ACHKW0_003996"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0032049 - cardiolipin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HFO7476745.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHKW0_003997"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="ACHKW0_003997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HFO7476746.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYIKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHKW0_003998"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="ACHKW0_003998"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HFO7476747.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLELVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMASDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHKW0_003999"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="ACHKW0_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HFO7476748.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /gene="yfiF"
                     /locus_tag="ACHKW0_004000"
     CDS             7531..8568
                     /gene="yfiF"
                     /locus_tag="ACHKW0_004000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HFO7476749.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHKW0_004001"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="ACHKW0_004001"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HFO7476750.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="ACHKW0_004002"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="ACHKW0_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HFO7476751.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHKW0_004003"
     CDS             complement(10049..10636)
                     /gene="eamB"
                     /locus_tag="ACHKW0_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HFO7476752.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10739..11620
                     /gene="yfiE"
                     /locus_tag="ACHKW0_004004"
     CDS             10739..11620
                     /gene="yfiE"
                     /locus_tag="ACHKW0_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7476753.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     LSIESIKRCVAANIGVSYLPRFAVEKELESGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         2920 a         2934 c         3036 g         2854 t
ORIGIN      
        1 aaaggccatc agtcacgatg gcctttttgc gttggcgcag aaaaaaatgc ctgatgcgac
       61 gctgcgcgtc ttatactccc acatatgcca gattcagcaa cgaatacggc ttccccaact
      121 tgctcacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataatca
      181 ttttcctgct aactaaattc atggttaagg ttgcataatg atatgcaaca aatgtataac
      241 attttcttta ccaaaagaaa taaacaaaag tgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc gattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acattttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgttatcgg tgtgtatgat gtgtttcgga tcgctggtta tcgcctgcct cccaggttat
      661 gaaactatag gtacgtgggc tccggcatta ttgcttctcg ctcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac cacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgccaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggtctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagctccag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttta ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gatcagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccggattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatacaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgccgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataatc
     4861 gtcgtgcctc ttgcgaggaa tgttcgccac accagttcgt cagcagagaa acatacttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt aatatagtgc tcactgctgg cgtcatcgcg cagatcgagc gggttagaga
     5101 ttgccacatg tcctggcagt gcatcgcgca gtttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cctcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgctggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga agcgggaaag accaccattg gctgatagtc cgggttttgc
     6541 accaactcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga cgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcgccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcatgtca ggcacttttc cagagccgtc cggaagcgat tgttcgcgcc
     7921 tggtttatcc agagtgtaac gccgcgtttt aaagaagcct tgcgctggat ggcagcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagcg gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaatggca caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgtgtactg gcgctggaaa acgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggcgttgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgttggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggg aaaccgctgt tcaaaaccag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtt
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg cattttaccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gcaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcgg taatcagggt gtaagtccaa aaagcactta
    10621 aaagggtcgg tgtcacttgt tatctcctct gttttggtag acagtacccg acacggaaca
    10681 tttaaaaaaa tgaatgtttt tatattctag attcgatatt ttcgataggt ttggggttat
    10741 ggatctgcgc cgttttatta cgcttaaaac cgtggtggaa gagggttcct ttttgcgagc
    10801 ttcgcaaaaa ttgtgctgta cacaatcgac ggtgactttt catattcagc agcttgagca
    10861 ggagttttca gtccagttat ttgagaaaat tggccgacgg atgtgcctta cccgcgaagg
    10921 aaaaaagttg ctgccgcaca tttatgaact tacccgagtg atggatacgc ttcgtgaggc
    10981 tgccaaaaag gagtcagatc cggacgggga actacgcgtt gtttcgggcg aaacgttgct
    11041 ctcttaccga atgccgcagg tgttgcagcg ttttcggcaa cgcgcaccaa aagtgcgttt
    11101 atcgttgcag tcgctgaact gttacgtgat ccgtgatgcg ctactgaatg atgaggccga
    11161 tgtcggcgtc ttttatcgcg tagggaatga tgatgccctg aaccgacgag agttgggtga
    11221 acaatcgctg gtgctggtgg cttcaccgca aattgcagat gtcgatttta ctgagccggg
    11281 aagacataac gcctgtagct ttattatcaa cgaaccgcaa tgtgtcttcc ggcagatatt
    11341 tgagagcacg ctgcgtcagc ggcggatcac ggtggaaaac accattgagc tgttaagcat
    11401 tgaaagtatc aaacgttgtg tcgcggcgaa tatcggtgtc agttacttgc cccgctttgc
    11461 ggtggaaaaa gagcttgaga gcggagaatt aattgaattg ccttttggcg aacagtcgca
    11521 gaccattacg gccatgtgcg cacaccatgc tggaaaagcg gttagcccgg cgatgcacac
    11581 ttttattcag tgtgttgaag agagttttgt ggctggataa aaataatgtc gggattaaac
    11641 ccggcattta acttgtcgac aaagtgcgct ttattcatgc cggatgcggc gtgaacgcct
    11701 tatccggcct acaagatcgt gcaaattcaa tatattgcat gagc
//
LOCUS       DAZFXT010000134        11643 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.134, whole genome shotgun sequence.
ACCESSION   DAZFXT010000134 DAZFXT010000000
VERSION     DAZFXT010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11643
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.134"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..553)
                     /locus_tag="ACHKW0_004005"
     CDS             complement(<1..553)
                     /locus_tag="ACHKW0_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476754.1"
                     /translation="MIFRDSLRVLPYGRVDNDFFQIEERRSWNAGRYYWSNRRIFGYI
                     GITQSSNKELKDKSGREGFIRNQAARELKTIISNLLTELADRFFGSRSDDRKELLEQV
                     KREKELRKSAQQQARKSTQKSFSEALKNQTPVLDASLEAVKRLKTKLDKTDGSLDLNY
                     LKIIDSDLTNLDALRSEIKTPIKP"
     gene            672..1760
                     /locus_tag="ACHKW0_004006"
                     /pseudo
     CDS             672..1760
                     /locus_tag="ACHKW0_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
     gene            2096..3073
                     /gene="glaH"
                     /locus_tag="ACHKW0_004007"
     CDS             2096..3073
                     /gene="glaH"
                     /locus_tag="ACHKW0_004007"
                     /EC_number="1.14.11.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417145.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050498 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, with 2-oxoglutarate as one donor, and
                     the other dehydrogenated [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutarate dioxygenase GlaH"
                     /protein_id="HFO7476755.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTFIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNIDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYASNHYQTHQ"
     gene            3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHKW0_004008"
     CDS             3093..4361
                     /gene="lhgO"
                     /locus_tag="ACHKW0_004008"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HFO7476756.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRFDNCGKMLVATSPLEMERM
                     RALWERTAANGIEREWLNADELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQD
                     RGGEIIYNAEVSALNEHKNGVVIRTSQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPRPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            4384..5832
                     /gene="gabD"
                     /locus_tag="ACHKW0_004009"
     CDS             4384..5832
                     /gene="gabD"
                     /locus_tag="ACHKW0_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009013 - succinate-semialdehyde
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HFO7476757.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            5846..7126
                     /gene="gabT"
                     /locus_tag="ACHKW0_004010"
     CDS             5846..7126
                     /gene="gabT"
                     /locus_tag="ACHKW0_004010"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009448 - gamma-aminobutyric acid metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HFO7476758.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGNFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYASS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHNKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFDEAKQ"
     gene            7364..8764
                     /gene="gabP"
                     /locus_tag="ACHKW0_004011"
     CDS             7364..8764
                     /gene="gabP"
                     /locus_tag="ACHKW0_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015185 - gamma-aminobutyric acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HFO7476759.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            8785..9447
                     /gene="csiR"
                     /locus_tag="ACHKW0_004012"
     CDS             8785..9447
                     /gene="csiR"
                     /locus_tag="ACHKW0_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HFO7476760.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(9448..9897)
                     /gene="kbp"
                     /locus_tag="ACHKW0_004013"
     CDS             complement(9448..9897)
                     /gene="kbp"
                     /locus_tag="ACHKW0_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HFO7476761.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(9981..10139)
                     /gene="yqaE"
                     /locus_tag="ACHKW0_004014"
     CDS             complement(9981..10139)
                     /gene="yqaE"
                     /locus_tag="ACHKW0_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HFO7476762.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            10322..10621
                     /gene="ygaV"
                     /locus_tag="ACHKW0_004015"
     CDS             10322..10621
                     /gene="ygaV"
                     /locus_tag="ACHKW0_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HFO7476763.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            10631..11155
                     /gene="ygaP"
                     /locus_tag="ACHKW0_004016"
     CDS             10631..11155
                     /gene="ygaP"
                     /locus_tag="ACHKW0_004016"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HFO7476764.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRT"
     gene            complement(11202..11606)
                     /gene="stpA"
                     /locus_tag="ACHKW0_004017"
     CDS             complement(11202..11606)
                     /gene="stpA"
                     /locus_tag="ACHKW0_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HFO7476765.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2845 a         2888 c         3005 g         2905 t
ORIGIN      
        1 gaggtttgat aggcgtttta atttcactgc gcaacgcatc taagtttgta agatcactgt
       61 ctataatttt aagatagttt aaatctaacg aaccatcagt tttatcaagc ttagttttca
      121 gccttttaac agcctccaag gaagcatcaa gaactggtgt ctgattcttc aaagcttctg
      181 aaaaactttt ttgtgttgat tttcgagctt gttgttgagc agattttctt aactcttttt
      241 cacgcttaac ttgttctaaa agctctttac ggtcatcaga acgtgaacca aaaaatctat
      301 cagcaagttc agttaacaaa ttagatataa tagttttcag ttctcttgca gcctggtttc
      361 ttatgaatcc ctctctcccc gacttatctt tcagctcttt attactggat tgagtaattc
      421 caatataacc aaaaatcctt ctattagacc aataatatcg ccctgcattc catgaacgtc
      481 tttcttctat ctggaagaaa tcattatcta ctcgaccata aggtaatact ctcaagctat
      541 ccctaaaaat cattagtcct gcatactttt tggccttaag atcaaagtgg gaatgttcac
      601 gttcagtatg tgatgtattt tgtgaaagga attcaaacgt tcctatctga agctcaaatg
      661 ggccgacccc tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaatgc
      721 ctaaaacaac ccccaaaagt gaccaggtta ttatgctgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt acgatgcagc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 atttatttaa cccaaaatat catgaaaaag tcgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaagaa caaatgtcat cgctgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcttt ttcatttggt ggcgatctcc
     1081 aaacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcaaaa agacggatat
     1141 tgattaacat taaacacgat tttcctaaca atggcaatat tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcaggc
     1261 ctttagttta ctgggatggc caagtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtcgacgcg tgttggcgat gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctggcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta attataataa ataaaggaaa cgaagaagtt gaaggtgcct
     1561 ggcctaacaa attgcaacct ggaaaatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattattaa tacccgaaaa attatccccc ctggtaaagc atttatgctt agaggcggaa
     1681 ctctaaatat caatattcct gggcgtagcg ctcttctttt agggaaaaca ggagaaccgc
     1741 tgaactatct ctatttgtaa tttaatgtta tatctgcccc gctaaaacgg ggcagataat
     1801 atgtttagtt tactaacggt cattttgcat tgaagccatt tactgttttt tatcgaccag
     1861 ataatctgtt ctctgatggt aactccccct aacctattgc tttagttatt catttcctgt
     1921 ctcactttgc cttaataccc tacgttaaat gttactaatt tgttgctttt gatcacaata
     1981 agaaaacaat atgtcgcttt tgtgcgcatt tttcagaaat gtagatattt ttagattatg
     2041 gctacgaaat gagcatcgcc atgtcaccct acatctcata agaggatcgc ttctgatgaa
     2101 tgcactgacc gccgtacaaa ataacgctgt cgattcaggc caggactata gcggattcac
     2161 tttcatcccg tcggcgcaat ccccgcgtct gctggaactt accttcaccg aacagacgac
     2221 caaacagttt ctcgaacagg ttgccgaatg gcccgtacag gcgctggagt acaaatcctt
     2281 tctgcgtttt cgggtaggca aaattcttga cgatctgtgt gcgaatcagc tgcaaccatt
     2341 gctgttgaag accctgctaa accgtgctga aggtgcgctg ttgatcaatg cggtgggtat
     2401 cgatgatgtg gcgcaggcag atgagatggt gaagctggca acggcggtag cgcatctgat
     2461 tggtcgctca aatttcgatg cgatgagcgg tcagtattac gcgcgtttcg tggtgaaaaa
     2521 tatcgataac tcagacagct atctgcgtca gccgcaccgc gtgatggaac tgcacaacga
     2581 cggcacgtat gtcgaagaga tcaccgatta cgtgctgatg atgaaaatcg atgagcaaaa
     2641 catgcagggt ggaaattcgt tgctgctgca tctcgatgac tgggaacatc tggaccacta
     2701 tttccgccat ccactggcgc gtcgcccgat gcgctttgcc gcgccgccga gcaaaaacgt
     2761 cagcaaagat gttttccatc cagtgttcga cgttgatcaa cagggtcgcc cggtgatgcg
     2821 ctatatcgac cagttcgtcc agccaaaaga ttttgaagaa ggcgtgtggt tgagcgagct
     2881 ttccgatgcc attgaaacca gcaaaggcat tctttctgta cccgttcccg ttggcaaatt
     2941 cctgttgata aacaacctat tctggctgca cggtcgcgac cgctttactc cgcacccgga
     3001 tctgcgccgt gaactgatgc gtcagcgtgg ctatttcgct tacgcctcta accactacca
     3061 gacgcatcag taagcgcaaa ggaattgagc ggatgtatga ttttgtgatt attggcggcg
     3121 gcatcatcgg catgtcgacc gccatgcaac tgattgatgt ctatccggat gcccgcattg
     3181 cgttgctgga aaaagagtcc ggcccggcct gtcaccagac gggccacaac agcggcgtga
     3241 tccatgccgg ggtctattac acgcccggta gcctgaaggc acagttttgc ctggcgggaa
     3301 accgcgccac taaagccttt tgcgatcaaa acggcattcg ctttgacaac tgcggcaaga
     3361 tgctggtcgc cacgtctccg ctcgaaatgg aacggatgcg cgccttatgg gaacgcacag
     3421 cggcgaacgg tatcgagcgc gaatggctaa acgccgacga actgcgcgag cgcgaaccga
     3481 atatcaccgg gctcggcggt atttttgtgc cgtccagcgg cattgtcagc taccgcgaag
     3541 tgacagcggc gatggcaaaa atctttcagg acagaggcgg cgagattatt tataacgccg
     3601 aagtcagcgc ccttaatgag cataaaaacg gcgtggtgat acgtaccagt cagggcggtg
     3661 aatatgaagc atcgacgctg attagctgtt ccgggctgat ggctgaccgg ctggtgaaaa
     3721 tgctcggact cgaaccgggc tttatcatct gcccgttccg tggcgagtat ttccgccttg
     3781 cgccggagca taaccagatt gttaaccacc tgatttaccc catccccgac ccggcaatgc
     3841 cgtttttggg cgtgcatctc acccgcatga tcgacggcag cgtgaccgtc gggccaaacg
     3901 cggtgctggc tttcaaacgt gaaggctatc gcaagcgcga cttctcattt agcgacacgc
     3961 tggaaatttt gggctcgtcg gggattcgcc gggtgctgca aaaccatcta cgctcaggac
     4021 tgggcgagat gaaaaactcg ctgtgcaaaa gcggctatct gcggctggtg caaaagtatt
     4081 gtccccggct ttcgttaagc gatctccagc ccaggcccgc cggtgtgcgg gcgcaggcgg
     4141 tatcgccgga cggcaagctg attgacgatt ttctgtttgt caccaccccg cgcacgatcc
     4201 acacctgcaa tgcgccctcc ccggcagcga catcagcaat tcctattggt gcgcatattg
     4261 tcagcaaggt acaaacgctg ttggcaagcc agagtaaccc cggacgcacg ctgcgagcgg
     4321 cacgtagtgt ggatgcctta cacgccgcat ttaatcaata acctttgaaa acaggacgta
     4381 acaatgaaac ttaacgacag taacttattc cgccagcagg cgttgattaa cggggaatgg
     4441 ctggacgcca acaatggcga ggtcatcgac gtcaccaatc cggcgaacgg cgacaagctg
     4501 ggtagcgtgc cgaaaatggg cgcggatgaa acccgcgccg ctatcgacgc cgccaaccgc
     4561 gccctgccag cctggcgcgc gctcaccgcc aaagaacgcg ccaccattct gcgcaactgg
     4621 ttcaatttga tgatggagca tcaggacgat ttagcgcgcc tgatgaccct cgaacagggt
     4681 aaaccgctgg ctgaagcgaa aggcgaaatc agctacgccg cttcctttat tgagtggttt
     4741 gccgaagaag gcaaacgcat ttatggcgac accattcccg gtcatcaggc cgataagcgc
     4801 ctgattgtta tcaagcagcc gattggcgtt accgccgcca tcacgccgtg gaacttcccg
     4861 gcggcgatga ttacccgtaa agccggtccg gcgctggcgg caggctgcac gatggtgctg
     4921 aaacccgcca gtcagacgcc gttctctgcg ctggcgctgg cggagctggc gattcgcgcg
     4981 ggcattccgg ctggcgtatt taacgtggtc accggttcgg cgggcgcggt gggtaacgaa
     5041 ctgaccagca acccgctggt gcgcaaactg tcgtttaccg gttcgaccga aattggccgc
     5101 cagttaatgg aacagtgcgc gaaagacatc aagaaagtgt cgctggagct gggcggcaac
     5161 gcgccgttta tcgtctttga tgatgccgat ctcgacaaag ccgtggaagg cgcactggcc
     5221 tcgaaattcc gcaacgccgg gcaaacctgc gtctgcgcca accgtttata cgtgcaggac
     5281 ggcgtgtatg accgctttgc cgaaaaatta cagcaggcgg tgagcaaact gcacatcggc
     5341 gacgggctgg ataaaggcgt caccatcggg ccgctgatcg atgaaaaagc ggtagcaaaa
     5401 gtggaagagc atattgccga tgcgctggag aaaggtgcgc gtgtggtttg cggcggtaaa
     5461 gcggatgaac gcggcggcaa cttcttccag ccgaccattc tggtggacgt tccagccaac
     5521 gccaaagtgt cgaaagaaga gacgttcggc cccctcgccc cactgttccg ttttaaagat
     5581 gaagccgatg tgattgcgca agccaatgac accgagtttg gccttgccgc ctatttctac
     5641 gcccgtgatt taagccgcgt cttccgcgtg ggcgaagcgc tggagtacgg catcgtcggc
     5701 atcaataccg gcatcatttc caatgaagtg gccccgttcg gcggcattaa agcctcgggt
     5761 ctgggtcgtg aaggttcgaa gtatggcatc gaagattact tagaaatcaa atatatgtgc
     5821 atcggtcttt aactggagaa cgcgaatgag cagcaataaa gagttaatgc agcgccgcag
     5881 tcaggcgatt ccccgtggtg ttgggcaaat tcacccgatt ttcgctgacc gcgcggaaaa
     5941 ctgccgggtg tgggacgttg aaggccgtga gtatcttgat ttcgcgggcg ggattgcggt
     6001 gctcaatacc gggcacttgc atccgaaagt ggtggctgcg gtggaagcgc agttgaaaaa
     6061 actgtcgcac acctgcttcc aggtgctggc ctacgagccg tatctggagc tgtgcgagat
     6121 tatgaatcag aaggtgccag gcaattttgc caagaaaacg ctgctggtca ccacaggttc
     6181 tgaagcggtg gaaaacgcgg tgaaaatcgc ccgcgccgcc accaaacgta gcggcaccat
     6241 cgcttttagc ggcgcgtatc acgggcgcac gcattacacg ctggcgctga ccggcaaggt
     6301 gaatccgtac tctgcgggca tggggctgat gccgggtcat gtttatcgcg cgctttatcc
     6361 ttgcccgctg cacggcataa gcgaggatga cgctatcgcc agcatccacc ggatcttcaa
     6421 aaatgatgcc gcgccggaag atatcgccgc catcgtgatt gagccggttc agggcgaagg
     6481 cggtttctac gcctcgtcgc cagcctttat gcagcgttta cgcgctctgt gtgacgagca
     6541 cgggatcatg ctgattgccg atgaagtgca gagcggcgcg gggcgtaccg gcacgctgtt
     6601 tgcgatggag cagatgggcg ttgcgccgga tcttaccacc tttgcgaaat cgatcgcggg
     6661 cggcttcccg ctggcgggcg tcaccgggcg cgcggaagta atggatgccg tcgctccagg
     6721 cggtctgggc ggcacctatg cgggtaaccc gattgcctgc gtggctgcgc tggaagtgtt
     6781 gaaggtgttt gagcaggaaa atctgctgca aaaagccaac gatctggggc agaagttgaa
     6841 agacggattg ctggcaatag ccgaaaaaca cccggagatc ggcgacgtac gcgggctggg
     6901 ggcgatgatc gccattgagc tgtttgaaga cggcgatcac aacaagccgg acgccaaact
     6961 caccgccgag atcgtggctc gcgcccgcga taaaggcctg attcttctct cctgcggccc
     7021 gtattacaac gtgctgcgca tccttgtacc gctcaccatt gaagacgctc agatccgtca
     7081 gggtctggag atcatcagcc agtgttttga tgaggcgaag cagtagcgcc gctcctatgc
     7141 cggaggcgac gctgcgcgtc ttgtccggcc tacggggatc caggtcggat aaggcgttta
     7201 cgccgcatcc gacaatctgt acgtgaacag gaagaaatct atgttggccg ggtaaggcgg
     7261 agccgctctc cggcaaaaag aatcaataac aattatacgc gtgacccggc gcgggaaatg
     7321 tcggggcgct ctcccaagtg acacactttc gagaggattc aggatggggc aatcatcgca
     7381 accacatgag ttaggcggcg ggctgaagtc acgccacgtc accatgttgt ctattgccgg
     7441 tgttatcggc gcaagtctgt ttgtcggttc cagcgtcgcc atcgccgaag cgggcccggc
     7501 ggtattactg gcctatctgt tcgccgggct actggtggtt atgattatgc ggatgttggc
     7561 ggaaatggcg gttgccacgc ccgataccgg ttcgttttcc acctatgccg ataaagccat
     7621 tggtcgctgg gcgggctata ccatcggctg gttgtactgg tggttttggg tactggttat
     7681 cccgctggaa gccaacatcg ccgccatgat cctgcactca tgggttccag gcattcccat
     7741 ctggttattt tccctcgtca ttaccctcgc cttaactggc agtaacttat taagcgttaa
     7801 aaactacggc gaatttgagt tctggctggc gctgtgcaaa gtcatcgcta tcctggcctt
     7861 tattttcctt ggtgcagtcg caattagcgg tttttacccg tatgccgaag tgagcgggat
     7921 ctcaagattg tgggatagcg gcggctttat gcccaacggt ttcggtgcgg tattaagcgc
     7981 gatgttgatc accatgttct cgtttatggg cgcagaaatt gtcaccattg ccgccgcgga
     8041 atccgacacg ccggaaaaac atattgtccg cgccaccaac tcggttatct ggcgtatttc
     8101 tatcttctat ttatgttcta tttttgtcgt cgtggcatta attccgtgga atatgcccgg
     8161 actaaaagcc gtcggttctt atcgctcggt actggaattg ctcaatattc cccatgcgaa
     8221 attaatcatg gactgcgtga tattactttc cgtaaccagt tgcctgaact cggcgctgta
     8281 taccgcgtca aggatgctct actccttaag ccgtcgcggt gatgctcccg cggtaatggg
     8341 taaaatcaac cgcagtaaaa ccccgtacgt ggcggtgtta ctctccaccg gcgcggcatt
     8401 cttaacggtg gtggtgaact attacgcgcc tgcgaaggta tttaaatttc tgatcgacag
     8461 ctccggcgct atcgccctgc tggtttattt agtcatcgcc gtttcacagt tgcggatgcg
     8521 caaaattctg cgagcagaag gaagcgaaat tcgcttgcga atgtggcttt atccgtggct
     8581 cacctggcta gtcatcggct ttattacctt tgtgttggta gtgatgctat tccgtccggc
     8641 gcaacagtta gaagtgatct ccaccggctt attagcgata gggattatct gtaccgtgcc
     8701 aattatggct cgctggaaaa agctggtatt gtggcaaaaa acacccgttc ataatacgcg
     8761 ctgatcatga tcaggagtca caccatgacc attacgtctc tggatggcta tcgctggctg
     8821 aagaacgata ttattcgcgg taattttcaa ccggatgaaa aattacgaat gagtttgctg
     8881 acatcgcgtt atgcacttgg cgttgggccg ttacgggaag ctctttcgca actggtggcg
     8941 gaacggctgg tcacggtggt gaatcaaaaa gggtatcggg tggcgtctat gtcagagcag
     9001 gagttgctcg atattttcga cgcccgcgcc aatatggaag cgatgttagt gagtctggcg
     9061 attgcccgcg gtggcgatga gtgggaggca gacgttctcg caaaagcgca tctgctgagt
     9121 aagcttgagg cctgtgacgc cagcgagaaa atgcttgatg agtgggatct gcgtcatcag
     9181 gcgtttcata cggcaattgt ggcgggctgt ggttctcact atttgctgca aatgcgtgaa
     9241 cggttgtttg atctggcggc gcgctatcga tttatctggc tgcggcgaac ggtgctttcg
     9301 gtagaaatgc tggaggataa acacgatcag caccagaccc tgactgcggc ggtactggcg
     9361 cgagataccg cgcgcgccag tgagttaatg cgccagcatt tactgacgcc aattcccatt
     9421 atccagcagg cgatggctgg caattaacta ctcttccgga atacgcaaca cttgccccgg
     9481 ataaatttta tccgggcttt ttagcatcgg tttattcgct tcgaagattt tattgtacag
     9541 attagcgtta ccgtagacct gtttggaaat ggcactcaga gtgtcgccag acttaacggt
     9601 ataaaactgg ctggcagtgg ctggtgtcgc cgttttcacc tgatcatcaa cactggcaat
     9661 accggaaata ttcccaaccg caacaaggat tttctccttc gcctcctgac tcaggccgtc
     9721 accagtgacc gtcgctttgc cgtcggcaat ttgaatgttc actttatcgg catcaggtat
     9781 accggttttg ctcagatgct cctgcacctt cttcgcctga tcgtctttat cgtgctgacc
     9841 tgtaaccgcg tcccagagtt tttctccggc atctttcaca aaattgaaca gacccatagc
     9901 tacctcattc gttaacggaa acactcagaa agtgtagcag aggaacgcgg cgcgttaacg
     9961 ccgcgggggt gagcacaggt ttaatcgcgg gtttgcaccc agaacgcgtg aatcaaaccg
    10021 gggatatacc caagcagtgt cagcagaata ttaataatga acgcccaacc gaacccttta
    10081 ccgagcagca cgccgagcgg cggcagaatg atggtgatga cgattctcca gaaacccata
    10141 tgtactccct ataagaaaat tactcattgt ttaaaaagag attttatctc ttaagcgtag
    10201 taattttagc ggagcctgcc agtttcagct tacgctttta ctctcattta cgctgctgag
    10261 gctggtatat gttgcatttt tagtttagag tttacttaat ttagaaaata cttaaataaa
    10321 tatgactgaa ctcgcgcaat tacaggccag tgccgaacag gcagcggcct tattgaaagc
    10381 aatgagccat cctaaacgat tgctgattct gtgcatgctt agcggttccc ccggcaccag
    10441 cgcgggagag ctgacgcgca ttaccggact gagtgcctct gcgacatcac agcatctcgc
    10501 tcgtatgcgg gacgaagggc ttatcgacag ccaacgggat gcccaacgca ttctatattc
    10561 cattaaaaat gaggcggtaa atgccattat cgccaccctg aaaaatgtct attgtccgta
    10621 aggagtcact atggctttga caaccatttc gccgcatgat gcacaagaat taatcgcacg
    10681 cggcgcaaag ttaatcgata ttcgtgacgc tgatgaatat cttcgtgaac atattcctga
    10741 agcagatctg gctccattat ccgtgctgga acagtcaggt cttccaccta aattacgtcg
    10801 cgagcaaatt atattccact gccaggcagg taaacgcacc agtaataacg ccgataaatt
    10861 agcagcgatt gccgcccccg cagaaatatt tttactcgaa gatgggattg atggctggaa
    10921 aagagctgga ttgccagtag cggtaaataa atctcaaccc ttgccgttaa tgcgccaggt
    10981 gcagatcgct gcgggtggtt taatattaat cggcgttgta ctgggttata ccgtaaatag
    11041 cggtttcttc ttattaagtg gctttgttgg tgccgggtta ctgtttgcag gaatcagcgg
    11101 tttttgtgga atggcaaggt tgttagataa gatgccgtgg aaccaacgaa cttgagaagc
    11161 gacgccggac gcgccctagc agcgacatcc ggcctcagta attagatcag gaaatcgtcg
    11221 agagatttac cttctgccag cgcctgagca attggcttcg gtgtacggcc ctgaccggtc
    11281 caggttttag tttcaccgtt aacatcggtg aatttatatt tcgccggacg cggctggcgt
    11341 tttttaccag cgcgtggtgc agcagcagag ctattaccta ataactcttc cgggttaatt
    11401 ccgtcagcct tcatcagctc cagccaggtg ctaatttttt cctgacgctc agccagttca
    11461 cgctgctgct gttcttcttc ttcacgtctt tctttagtga caaccctgaa tttttcgagc
    11521 atttcttcaa gaacgtcaat ggagaattcg cgagccatcg cacggagagt gcgaatgtta
    11581 tttaaacttt gtaacattac ggacataata aagaaaacct tttaacgcca aaacaaaaaa
    11641 gta
//
LOCUS       DAZFXT010000135        11593 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.135, whole genome shotgun sequence.
ACCESSION   DAZFXT010000135 DAZFXT010000000
VERSION     DAZFXT010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11593
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.135"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..874
                     /gene="yjeJ"
                     /locus_tag="ACHKW0_004018"
     CDS             5..874
                     /gene="yjeJ"
                     /locus_tag="ACHKW0_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HFO7476766.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
     gene            complement(1077..1430)
                     /gene="yjeI"
                     /locus_tag="ACHKW0_004019"
     CDS             complement(1077..1430)
                     /gene="yjeI"
                     /locus_tag="ACHKW0_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HFO7476767.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1568..3214)
                     /gene="groL"
                     /locus_tag="ACHKW0_004020"
     CDS             complement(1568..3214)
                     /gene="groL"
                     /locus_tag="ACHKW0_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HFO7476768.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3258..3551)
                     /gene="groS"
                     /locus_tag="ACHKW0_004021"
     CDS             complement(3258..3551)
                     /gene="groS"
                     /locus_tag="ACHKW0_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HFO7476769.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3827..5083
                     /gene="yjeH"
                     /locus_tag="ACHKW0_004022"
     CDS             3827..5083
                     /gene="yjeH"
                     /locus_tag="ACHKW0_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HFO7476770.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            complement(5099..5575)
                     /gene="fxsA"
                     /locus_tag="ACHKW0_004023"
     CDS             complement(5099..5575)
                     /gene="fxsA"
                     /locus_tag="ACHKW0_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HFO7476771.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            5912..7348
                     /gene="aspA"
                     /locus_tag="ACHKW0_004024"
     CDS             5912..7348
                     /gene="aspA"
                     /locus_tag="ACHKW0_004024"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008797 - aspartate ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HFO7476772.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7466..8767
                     /gene="dcuA"
                     /locus_tag="ACHKW0_004025"
     CDS             7466..8767
                     /gene="dcuA"
                     /locus_tag="ACHKW0_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HFO7476773.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8883..9221
                     /gene="cutA"
                     /locus_tag="ACHKW0_004026"
     CDS             8883..9221
                     /gene="cutA"
                     /locus_tag="ACHKW0_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HFO7476774.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9197..10894
                     /gene="dsbD"
                     /locus_tag="ACHKW0_004027"
     CDS             9197..10894
                     /gene="dsbD"
                     /locus_tag="ACHKW0_004027"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HFO7476775.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10931..11506
                     /gene="yjdC"
                     /locus_tag="ACHKW0_004028"
     CDS             10931..11506
                     /gene="yjdC"
                     /locus_tag="ACHKW0_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7476776.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2771 a         3009 c         2873 g         2940 t
ORIGIN      
        1 ctacatggcc ataagcatca agggtgttaa taccggtgtt attcgcaaga gtaataattt
       61 cattgcactg gcgctgaaaa ttaaagaacc tcgcaataaa gaatcgttgt tctttatgtc
      121 tgtcatggaa ctgcgcgacc tgctgattgc gctggaaagt cgtatgcatc aaaagcataa
      181 actggatgct gctgcacgcc ttcagtatga gcaagctcgc gataaagtca ttaaaaaaat
      241 ggcagaaaac atcccagaaa ttctggttga tgagcttaaa aatgccgata ttaatcgtcg
      301 ggttaacacg ctggaactta ccgataatca aggcgaaaat cttaccttcg tattaacgct
      361 acatgacggt agcaaatgtg agttagtcgt caatgagttg cagattgaaa tgctggcgcg
      421 agcaatcatt catgcaatta ataatgcgga aatgcgtgaa ctggcattac gtattacctc
      481 actgttagat ttcctgccgt tatatgacgt tgactgccag gaaaatggca atctggaata
      541 tgacacctat tcccaaccgg aatggaaaca taacctgttc gaccattatc tggcggtgct
      601 ttaccgcttt aaagatgaaa gtggcaaaga acaatttagc ggcgcagtgg tgaaaacgcg
      661 tgaagcaacg ccgggtaaag aaatagaggc gatcactcgc cgaatgcttg atttcagccc
      721 acgactgaaa aaactcgcgg gtgtgccatg tcaggtctat gttcgcaccg tagcggcgaa
      781 taacgcccaa ccgctaaccc aggatcaatg cttacgcgcg ctgcatcact tacgtgttca
      841 atccaccagc aaaactgcac cacaggcgaa ataatctcat ctttgctaat gcgaggctgg
      901 agccatccag cctgaaatga ctgataacgt gatcgttggt catgccggat gcggcgtaaa
      961 cgccttatcc ggcctacaaa ttctcaagca ttcaatcagt tgcaatgcct gctgtaggcc
     1021 tgataagcga agcgcatcag gcaatgatac gtttgtcacc cagcctctgg atcacatcag
     1081 ttcgggcact tataaacctg accgataatc tggctatccg tcgggacaaa actggacaac
     1141 attccctgcg acgggctgct gatgccataa atcacgttac cgcccattgc agccgcctgg
     1201 ttgcgcagat cgtttgctgc gccgcgcata gaaccgccct cttctccgtg ttgcccggaa
     1261 agccagttgc tttgcttacc tgtcgcagta ccaatcagct ggcactctgc gcctggctgc
     1321 tcgtccacaa tgcgtacact ctgaccggca gcactcaatt cgttgctgga gctacaaccc
     1381 gccatcagta gcgcggcacc gacaatccct gctaagtatt ttacgtgcat gttattcccc
     1441 ataatcaatg agctggacgc cactcgcgtc gtccgtgtct gaatcttata ctagaaagat
     1501 gaccaaaaga aaaaccccca gacatttctg cccgggggtt tgtttatttc tgcgaggtgc
     1561 agggcaatta catcatgccg cccatgccac ccatgccgcc cataccgcca gcagcgccta
     1621 agtcagctgc atcatttttc ggcaggtcgg taaccatgca ctcggtggtg atcatcaggc
     1681 cagccacaga agccgcgtac tgcagagcag aacgggttac tttggttggg tccaggatac
     1741 ccatgtcgat catgttgccg tattcttcgg ttgctgcgtt gtaaccgtag ttgccgtcgc
     1801 cgcctttaac ggtgttagca acaacagacg gttcttcgcc gcagttcagg acgatctgac
     1861 gcagcggagc ttccattgca cgcagtgcaa ctttgatacc cacgttctgg tcttcgttct
     1921 gaccacgcag gtcagccagt ttagacgcta cgcggatcag cgcaacacca ccaccagcaa
     1981 ccacgccttc ttctaccgca gcacgggtcg cgtgcagggc gtcttcaacg cgtgcttttt
     2041 tctctttcat ttcaacttcg gtagcagcac ctactttgat aactgcaacg ccgcctgcca
     2101 gtttcgctac gcgttcctgc agtttttcac ggtcgtagtc agaagttgct tcttcaatct
     2161 gctgacggat ctgagcaaca cggccctgga ttgcagcttc ttcacccacg ccatcgatga
     2221 tagtggtggt gtctttgttg atcacaacac gtttagcctg acccaggtct tccagggttg
     2281 ctttttccag ctccataccg atctcttcag agattacggt accgccagtc agggttgcga
     2341 tatcctgcag catagcttta cgacgatcgc cgaagcccgg agctttaacc gcagcaactt
     2401 tcacgatgcc acgcatggtg ttaacaacca gagttgccag cgcttcgcct tcaacatctt
     2461 cagcgatgat cagcagcggt ttgcctgctt tggcaacagc ttccagaacc ggcagcattt
     2521 cgcggatgtt agagattttc ttgtcagcca gcaggatgaa cgggctttcc agttctactg
     2581 cgccagtttc cggcttgttg atgaagtaag gagacaggta gccacggtcg aactgcatac
     2641 cttcaaccac gtccagttcg tcctgcagac cggtaccgtc ttcaacggtg ataacgcctt
     2701 ctttaccgac tttgtccatc gcttcagcga tcagtttacc tacggtttcg tcggagttag
     2761 cggagatggt accaacctga gcaatcgctt tagagtcaga gcacggtacg gacagcgctt
     2821 tcagttcttc aactgcagcg gtaacggctt tgtcgatacc acgtttcagg tccatcgggt
     2881 tcatgcccgc agcaacagct ttcagaccct cagtgatgat agcctgagcc agtacagttg
     2941 cagtggtggt accgtcgcct gcagcgtcgt tcgctttaga ggcaacttct ttcaccatct
     3001 gcgcacccat attttcgaac ttgtcttcca gttcgatttc acgagcaacg gaaacaccat
     3061 ctttggtgat ggtcggtgca ccgaaagatt tatccagaac tacgttacgg cctttcggac
     3121 cgagggtaac tttcactgca tctgccagta cgtttacgcc gcgcagcatt ttcacacgag
     3181 cgtcgttacc gaattttacg tctttagctg ccattatctt tattccttaa attcgtatgt
     3241 tcagtgtcgt gcgaggatta cgcttcaaca attgccagaa tgtcgctttc ggacatgatc
     3301 aacacttctt cattgtcgat cttctcagat ttcacaccgt agccatcgtt gaaaataacg
     3361 atgtcgccaa ctttcacatc cagcggcttc acttcgccat tttcaaggat acggccattg
     3421 ccgacagcca gcacttcgcc gcgggtggat ttagccgctg cagagccggt cagaacgatg
     3481 ccgccagcag atttagtttc aacttcttta cgcttgacga tcacgcgatc atgcaatgga
     3541 cgaatattca ttgataactc tcctttgaga aagtccgtat ctgttatggg tgacgccgga
     3601 gcttacgtgg tttcccggct ggtgaccaga gaaatgggga tgaggcttcg ccccttcaag
     3661 ggggaaaaag aaaaaaaatt ctgatcagca caaaatcggg tgaaaaccct gattcacctc
     3721 acatttcatc gcaatttctt catcgccgtc ataagcgaat ctgattgtgc taccatcgaa
     3781 aatctacgca gttgcccaaa atttgggcgc aatcggatat caacccatga gtggactcaa
     3841 acaagaactg gggctggccc aaggcattgg cctgctatcg acgtcattat taggcactgg
     3901 cgtgtttgcc gttcctgcgt tagctgcgct ggtagcgggc aataacagcc tgtgggcgtg
     3961 gcccgttttg attatcttag tgttcccgat tgcgattgtg tttgcgattc tgggtcgcca
     4021 ctatcccagc gcaggcggtg tcgcgcactt cgtcggtatg gcgtttggtt cgcgacttga
     4081 gcgagtcacc ggctggttgt ttttatcggt cattcccgtg ggtttgcctg ccgcgctaca
     4141 aattgccgcc gggttcggcc aggcaatgtt tggctggcat agctggcaac tgttgttggc
     4201 agaactcggt acgctggcgc tggtgtggta tatcggtact cgcggtgcca gttccagtgc
     4261 taatctacaa accgttattg ccggacttat cgtcgcgctg attgtcgcta tctggtgggc
     4321 gggcgatatc aaacctgcga atatcccctt cccggcacct ggtaatatcg aacttaccgg
     4381 gttatttgct gcgttatcag tgatgttctg gtgttttgtc ggtctggaag catttgccca
     4441 tcttgcctcg gaatttaaaa atccagagcg tgattttcct cgcgcattga tgattggtct
     4501 gctgctggca ggattagtct actggggctg tacggtagtc gtcttacact tcgacgccta
     4561 tggtgaaaaa atggcggcgg cagcatcgct tccaaaaatt gtagtgcagt tgttcggtgt
     4621 aggagcgtta tggattgcct gcgtgattgg ctatctggcc tgctttgcca gtctcaacat
     4681 ttatatacag agcttcgccc gcctggtctg gtcgcaggcg caacataatc ctgaccacta
     4741 cctggcacgc ctctcttctc gccatatccc gaataatgcc ctcaatgcgg tgctcggctg
     4801 ctgtgtggtg agcacgttgg tgattcatgc tttagagatc aatctggacg ctcttattat
     4861 ttatgccaat ggcatcttta ttatgattta tctgttatgc atgctggcag gctgtaaatt
     4921 attgcaagga cgttatcgac tactggcggt ggttggcggg ctgttatgcg ttctgttact
     4981 ggcaatggtc ggctggaaaa gtctctatgc gctgatcatg ctggcggggt tatggctgtt
     5041 gctgccaaaa cgaaaaacgc cggaaaatgg cataaccaca taatccggcg tttcgacatt
     5101 aatcctggcg atcgtcttta tgatcaaggc ggtcgcgctc atcatccttt cgctggtact
     5161 caccatcaaa agtattaccg ccaccggtcc cggcgctaaa accgccgcct ggcatacgag
     5221 aaaagcgcaa atgcggcatc aacttcactg tcagatgctt ttgcaccggc ggtaataaaa
     5281 gcagaagacc gaggaagtcg gtgaaaaagc ccggtaataa aagcagcaaa ccggcaatga
     5341 tcagcgaaac acttttaatc atctccgccg ctgggttttc acccgccgcc attttttgct
     5401 gcatcagcac gaaattctta aagccctggt tacgtaccag tgacatcccg ataactgacg
     5461 tgaatataac cagcacgagg gtcagcaata cccccaatac atgggcaacc tgaataaaga
     5521 ttgaaatctc aatatagaca taaaggaaaa tggcaataaa aggtaaccag cgcaaaggtt
     5581 tctcctgtaa tagcagccgg taaaacccgg ctacctgaat gggttgcgaa tcgcgttcag
     5641 cttatattgt ggtcattagc aaaatttcaa gatgtttgcg caactatttt tggtagtaat
     5701 cccaaagcgg tgatctattt cacaaattaa taattaaggg gtaaaaaccg acacttaaag
     5761 tgatccagat tacggtagaa atcctcaagc agcatatgat ctcgggtatt cggtcgatgc
     5821 aggggataat cgtcggtcga aaaacattcg aaaccacata tattctgtgt gtttaaagca
     5881 aatcattggc agcttgaaaa agaaggttca catgtcaaac aacattcgta tcgaagaaga
     5941 tctgttgggt accagggaag ttccagctga tgcctactat ggtgttcaca ctctgagagc
     6001 gattgaaaac ttctatatca gcaacaacaa aatcagtgat attcctgaat ttgttcgcgg
     6061 tatggtaatg gttaaaaaag ccgcagctat ggcaaacaaa gagctgcaaa ccattcctaa
     6121 aagtgtagcg aatgccatca ttgccgcatg tgatgaagtc ctgaacaacg gaaaatgcat
     6181 ggatcagttc ccggtagacg tctaccaggg cggcgcaggt acttccgtaa acatgaacac
     6241 caacgaagtg ctggccaata tcggtctgga actgatgggt caccagaaag gtgaatatca
     6301 gtacctgaac ccgaacgacc atgttaacaa atgtcagtcc actaacgacg cctacccgac
     6361 cggtttccgt atcgcagttt actcttccct gattaagctg gtagatgcga ttaaccaact
     6421 gcgtgaaggc tttgaacgta aagctgtcga attccaggac atcctgaaaa tgggtcgtac
     6481 ccagctgcag gacgcagtac cgatgaccct cggtcaggaa ttccgcgctt tcagcatcct
     6541 gctgaaagaa gaagtgaaaa atatccaacg taccgctgaa ctgctgctgg aagttaacct
     6601 tggcgcaaca gcaatcggta ctggtctgaa cacgccgaaa gagtactctc cgctggcagt
     6661 gaaaaaactg gctgaagtca ctggcttccc atgcgtaccg gctgaagacc tgatcgaagc
     6721 gacctctgac tgcggcgctt atgttatggt tcacggcgcg ctgaaacgcc tggctgtgaa
     6781 gatgtccaaa atctgtaacg acctgcgctt gctctcttct ggtccacgtg ccggcctgaa
     6841 cgagatcaac ctgccggaac tgcaggcggg ctcttccatc atgccagcta aagtaaaccc
     6901 ggttgttccg gaagtggtta accaggtatg cttcaaagtc atcggtaacg acaccactgt
     6961 taccatggca gcagaagcag gtcagctgca gttgaacgtt atggagccgg tcattggcca
     7021 ggccatgttc gaatccgttc acattctgac caacgcttgc tacaacctgc tggaaaaatg
     7081 cattaacggc atcactgcta acaaagaagt gtgcgaaggt tacgtttaca actctatcgg
     7141 tatcgttact tacctgaacc cgttcatcgg tcaccacaac ggtgacatcg tgggtaaaat
     7201 ctgtgccgaa accggtaaga gtgtacgtga agtcgttctg gaacgcggtc tgttgactga
     7261 agcggaactt gacgatattt tctccgtaca gaatctgatg cacccggctt acaaagcaaa
     7321 acgctatact gatgaaagcg aacagtaatc gtacagggta gtacaaataa agaaggcacg
     7381 tcagatgacg tgcctttttt cttgtgagca gtaacttaaa aataacaacc taatatcaac
     7441 ttgttaaaaa acaaggaagg ctaatatgct agttgtagaa ctcatcatag ttttgctggc
     7501 gatcttcttg ggcgccagat tggggggaat aggtattggt tttgcaggcg gattgggggt
     7561 gctggttctt gccgctattg gcgttaaacc cggtaacatc ccgttcgatg tcatttccat
     7621 tatcatggcg gttatcgccg ctatttctgc catgcaggtt gctggcggtc tggactatct
     7681 ggttcatcag acagaaaagc tgctgcgccg taacccgaaa tacatcacga tcctcgcacc
     7741 gatcgtgacc tatttcctga ctatctttgc tggtactggc aacatatctc tggcgacact
     7801 gccagttatc gctgaagttg cgaaggaaca aggcgtcaaa ccttgccgtc cgctgtctac
     7861 tgcagtggta tccgcgcaga ttgcgatcac cgcatcgcca atctcagcgg cagtggttta
     7921 catgtcttcc gtgatggaag gtcatggcat cagctacctc catctgctct ccgtggtcat
     7981 cccgtccacc ctgctggcgg ttctggtgat gtcctttctg gtcactatgc tattcaactc
     8041 caaactctct gacgatccga tttatcgcaa gcgtctggaa gagggcctgg ttgaactgcg
     8101 cggtgaaaag cagattgaaa tcaaatccgg tgcaaaaacg tccgtctggc tgttcctgct
     8161 gggcgtagtt ggcgtggtta tctatgcaat catcaacagc ccaagcatgg gtctggttga
     8221 aaaaccgctg atgaacacca ccaacgcaat cctgatcatc atgctcagcg ttgcaactct
     8281 gaccaccgtt atctgtaaag tcgataccga caacatcctc aactccagca ccttcaaagc
     8341 aggtatgagc gcctgtattt gtatcctggg tgttgcgtgg ctgggcgata ctttcgtttc
     8401 caacaacatc gactggatca aagataccgc tggtgaagtg attcagggtc atccgtggct
     8461 gctggccgtc atcttcttct ttgcttctgc tctgctgtac tctcaggctg caaccgcaaa
     8521 agcactgatg ccgatggccc tggcactgaa cgtttcaccg ctgaccgctg ttgcttcttt
     8581 cgctgcggtg tctggtctgt tcattctgcc gacctacccg acgctggttg ctgcggtaca
     8641 gatggatgac acgggtacaa cccgtatcgg taaattcgtc ttcaaccatc cgttcttcat
     8701 cccgggtact ctgggtgttg ccctggccgt ttgcttcggc ttcgtgctgg gtagcttcat
     8761 gctgtaatga ccaatcgcgg ggcgttcacg ccccgctttc tttcccgccg actaacatcc
     8821 ttcccccgtc cgttgtatag tgacctctct cttgcggttc catctgttct tgcgaggtgt
     8881 ttatgcttga tgaaaaaagt tcgaataccg cgtctgtcgt ggtgctatgt accgcaccgg
     8941 atgaagcgac agcccaggat ttagccgcca aagtgctggc ggaaaaactg gcggcctgcg
     9001 cgaccttgat ccccggcgct acctctctct attactggga aggtaagctg gagcaagaat
     9061 acgaagtgca gatgatttta aaaactaccg tatctcacca gcaggcactg ctggaatgcc
     9121 tgaagtctca tcatccatat caaaccccgg aacttctggt tttacctgtt acacacggag
     9181 acacagatta cctctcatgg ctcaacgcat ctttacgctg atcctgctac tttgcagcac
     9241 ttccgttttt gccggattat tcgacgcgcc gggacgttca caatttgtcc ccgcggatca
     9301 agcctttact tttgattttc agcaaaacca acatgacctt aatctgacct ggcagatcaa
     9361 agacggttac tacctctacc gtaaacagat ccgcattacg ccggaacacg cgaaaattgc
     9421 cgacgtgcag ctgccacaag gcgtctggca tgaagatgag ttttacggca aaagcgagat
     9481 ttaccgcgat cggctgacgc ttcccgtaac catcaaccag gcgagtgcgg gagcgacgtt
     9541 aactgtcacc taccagggct gtgctgatgc cggtttctgt tatccgccag aaaccaaaac
     9601 cgttccgtta agcgaagtgg tcgccaacaa cgcagcgtca cagcctgtgt ctgtttcgca
     9661 gcaagagcag cacaccgcgc aattgccctt ttccgcgctc tgggcgttgt tgatcggtat
     9721 tggtatcgcc tttacgccat gcgtgctgcc aatgtaccca ctgatttctg gcatcgtgct
     9781 gggtggtaaa cagcggctct ccactgccag agcattgttg ctgaccttta tttatgtgca
     9841 ggggatggca ctgacttaca cggcgctggg tctggtagtt gccgccgcag ggttacagtt
     9901 ccaggcggcg ctacagcacc catacgtgct cattggcctc gccatcgtct ttaccttgct
     9961 ggcgatgtca atgtttggct tgtttactct acaactcccc tcttcgctgc aaacacgtct
    10021 cacgttgatg agcaatcgcc aacagggcgg ctcacctggc ggtgtgtttg ttatgggggc
    10081 gattgccgga ctgatctgtt caccatgcac caccgcaccg cttagcgcga ttctgctgta
    10141 tatcgcccaa agcgggaaca tgtggctggg cggcggcacg ctttatctct atgcgttggg
    10201 catgggcctg ccgctgatgc taattaccgt ctttggtaac cgcttgctgc cgaaaagcgg
    10261 cccgtggatg gaacaagtca aaaccgcgtt tggttttgtg atcctcgcac tgccggtctt
    10321 cctgctggag cgagtgattg gtgatgtatg gggattacgc ttgtggtcgg cgctgggtgt
    10381 cgcattcttt ggctgggcct ttatcaccag cctacaggct aaacgcggct ggatgcgcgt
    10441 ggtgcaaata atcctgctgg cagcggcatt ggttagcgtg cgcccacttc aggattgggc
    10501 atttggtgcg acgcataccg cgcaaactca gacgcatctc aactttacac aaatcaaaac
    10561 ggtagatgag ttaaatcagg cgctcgttga agccaaaggc aaaccggtga tgttagatct
    10621 ttatgccgac tggtgcgtcg cctgtaaaga gtttgagaaa tacaccttca gcgacccgca
    10681 ggtgcaaaaa gcgttagcag acacggtctt acttcaggcc aacgtcacgg ccaacgacgc
    10741 acaagatgtg gcgctgttaa agcatcttaa tgtccttggc ctaccgacaa ttctcttttt
    10801 tgacggacaa ggccaggagc atccacaagc acgcgtcacg ggctttatgg atgctgaaac
    10861 cttcagcgca catttgcgcg atcgccaacc gtgaacgaca cttgcagtgg gaaagacgga
    10921 ggaaaatagc gtgcaacgtg aagatgtact gggagaagcc ctgaaattat tagaattaca
    10981 agggattgcc aacaccacgc tggagatggt tgctgaacgt gtggattatc cactggacga
    11041 gctacgccgc ttctggccag acaaagaggc aatcctctac gatgcgctgc gttatcttag
    11101 ccaacagata gatgtctggc gtcgtcagct gatgctggac gaaacgcaaa ccgccgaaca
    11161 aaagttgctg gcacgttatc aggcgttatc ggagtgcgtt aaaaacaacc gctatccggg
    11221 ctgtctattt atcgctgcct gtacgtttta tcccgatcct ggccacccta ttcatcaact
    11281 ggccgatcag caaaaaagcg cggcctacga tttcacccac gaactgttaa ccacgctgga
    11341 agttgacgat ccggcgatgg tagcaaagca gatggaactg gtgctggaag gctgtttaag
    11401 ccgaatgctg gtgaatcgta gccaggcgga tgtcgacact gcacatcggc tggcggaaga
    11461 tatcctgcgc ttcgcccgct gccgccaggg tggtgcattg acctgacaga aacacagaaa
    11521 agaagcgatt tgccgcaatc ttaagcagtt gaatcgcttt tactgaaatt aggttgacga
    11581 gatgtgcaga tta
//
LOCUS       DAZFXT010000136        11566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.136, whole genome shotgun sequence.
ACCESSION   DAZFXT010000136 DAZFXT010000000
VERSION     DAZFXT010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.136"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..6935
                     /gene="yeeJ"
                     /locus_tag="ACHKW0_004029"
                     /pseudo
     CDS             1..6935
                     /gene="yeeJ"
                     /locus_tag="ACHKW0_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
     gene            complement(7197..8249)
                     /locus_tag="ACHKW0_004030"
     CDS             complement(7197..8249)
                     /locus_tag="ACHKW0_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HFO7476777.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEKTKLTLVTMTMQDHATLKEIKDLAK
                     NITQKYGTPDLCIEAMRKKNLKTMIFISQLKAKTNFQVVGLTMKCYSPLCKNASRMDQ
                     NLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIALNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARVLVDCYNNVYRLSLSPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
     gene            8564..9880
                     /gene="shiA"
                     /locus_tag="ACHKW0_004031"
     CDS             8564..9880
                     /gene="shiA"
                     /locus_tag="ACHKW0_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HFO7476778.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDNQRA"
     gene            9982..11436
                     /gene="amn"
                     /locus_tag="ACHKW0_004032"
     CDS             9982..11436
                     /gene="amn"
                     /locus_tag="ACHKW0_004032"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HFO7476779.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         3099 a         2827 c         2763 g         2877 t
ORIGIN      
        1 ggtaaacgcc gcaacccaac aaccagttcc tgcacaactc gccattgcaa atgccaatac
       61 ggtgccctac acccttggag cgctggaaat cggcccaaag cgttgccgaa cgtttcggta
      121 tttcggtggc tgagttacgc aaactcaacc agtttcgtac gtttgctcga ggttttgata
      181 atgtccgcca gggtgatgaa ctggatgtcc cggcacaagt tagtgaaaat aatttaaccc
      241 cgccaccggg taatagcagc ggcaaccttg agcaacagat agccagtact tcacagcaaa
      301 tcgggtctct gctcgccgag gatatgaaca gcgagcaagc ggcaaatatg gcgcgtggat
      361 gggcctcttc tcaggcttca ggcgcaatga cagactggtt aagccgcttc ggtaccgcaa
      421 gaatcacgct gggcgtggat gaagatttta gcctgaagaa ctcccagttc gattttctcc
      481 atccgtggta tgaaacgcct gataatctct ttttcagtca gcatactctc catcgtactg
      541 acgagcgtac acagattaac aacggcttag gttggcgtca tttcactccc acatggatgt
      601 cgggcatcaa cttctttttc gaccacgatc ttagccgtta ccactcccgc gccggcattg
      661 gcgcggagta ctggcgcgac tatctaaaat taagcagtaa cggctatttg cgactgacca
      721 actggcgcag cgcacctgaa ctggacaacg attatgaagc acgcccggcc aatggctggg
      781 atgtacgcgc agaaggctgg ctacccgcct ggccgtacct tggcggtaaa ctggtctatg
      841 aacagtatta tggcgatgaa gtggccctgt tcgataaaga cgatcggcaa agtaatcctc
      901 atgccataac cgctggactt aactataccc ccttcccgct aatgaccttc agcgcggagc
      961 aacgccaggg taaacagggc gaaaatgaca cccgttttgc cgtcgatttt acctggcaac
     1021 ctggcagcgc aatgcagaaa cagcttgacc cgaatgaagt cgctgcacgg cgtagccttg
     1081 caggcagccg ttatgatctg gtggatcgca acaacaacat cgttctggaa tatcgcaaaa
     1141 aagaactggt ccgcctgacc ctgacagacc ccgtgacagg gaagtcagga gaagtgaaat
     1201 cactggtttc gtcgctacaa accaaatatg ccctgaaagg ctataacgtc gaagccaccg
     1261 cactggaagc tgccggtggc aaagtggtca caacgggtaa agatattctg gttaccctgc
     1321 cgggttaccg gttcaccagt acgccagaaa ccgataacac ctggccgatt gaagtcaccg
     1381 ctgaagatgt caaaggcaat ttgtcgaatc gtgaacaaag catggtggtc gttcaggcac
     1441 ctacgctaag ccagaaagat tcctcggtat cgttaagtac ccaaacattg aacgcggatt
     1501 cccattcaac cgccacactg acttttattg cgcatgatgc agcaggtaat cctgttgtcg
     1561 ggctggtgct ctcgacgcgt cacgaaggtg ttcaggacat caccctttct gaatggaaag
     1621 ataatggtga cggaagctat acccagatcc tgaccacagg agcgatgtct ggcacgctga
     1681 cgctgatgcc acagctgaat ggtgtggatg cggctaaagc ccccgccgtg gtgaatatca
     1741 tttctatttc gtcatcccga actcactcgt caattaaaat tgataaagac cgttatctct
     1801 ccggcaatcc tatcgaggtg acggtagaac tgagagatga aaatgacaaa cctgttaagg
     1861 agcaaaaaca gcaactgaat aacgcagtca gcatcgacaa cgtgaaacct ggtgtcacta
     1921 cagactggaa agaaaccgca gatggcgtct ataaggcaac ctataccgcc tataccagag
     1981 gcagtgggct tactgcgaag ctgttaatgc aaaactggaa tgaagatttg cataccgctg
     2041 gatttatcat cgacgccaac ccgcagtcag caaaaattgc gacattatct gccagcaata
     2101 atggtgtgct cgccaatgag aatgcagcaa acaccgtctc ggtcaatgtc gctgatgaag
     2161 gaagcaaccc aatcaatgat cataccgtca cgtttgcggt attaagcggg tcggcaactt
     2221 gctttaacaa tcaaaacacc gcaaaaacgg atgttaatgg tctggcgact tttgatctga
     2281 aaagtagtaa gcaggaagac aacacggttg aagtcaccct tgaaaatggc gtgaaacaaa
     2341 cgttaattgt cagttttgtc ggcgactcga gtaccgcgca ggttgatctg cagaagtcga
     2401 aaaatgaagt ggtcgctgac ggcaatgata gcgccacaat gaccgcgaca gtccgggatg
     2461 caaaaggcaa cctgctcaat gacgtcaagg tcactttcaa tgttaattca gcagcagcga
     2521 aactgagcca aaccgaagtg aatagccacg acgggatcgc cacagctacg ctgaccagtt
     2581 tgaaaaatgg tgattatagg gttacggcct ctgtgagctc tggttctcag gctaatcaac
     2641 aggtgatttt tatcggtgat caaagtactg ctgccctgac cctcagtgtg ccttcaggtg
     2701 atatcaccgt caccaacaca gctccgctac atatgactgc aaccttgcag gataaaaatg
     2761 gcaacccatt aaaagataaa gaaatcacct tctctgtgcc aaacgacgtc gcaagtcggt
     2821 tctcgattag caacagcgga aaaggcatga cggatagcaa cgggactgca atcgcctccc
     2881 tgaccggcac gttagcgggc acgcatatga tcacggctcg tctggctaac agcaatgtca
     2941 gcgatacaca gccaatgacg tttgtggcgg ataaagacag agcggttgtc gttctgcaaa
     3001 catcgaaagc ggaaatcatt gggaatggcg tggatgagac aactctgaca gcaacagtga
     3061 aagatccgtc gaatcatccg gtggcgggga taacggtcaa cttcaccatg ccacaggacg
     3121 ttgcggcaaa ctttaccctt gaaaataacg gtattgccat cactcaggcc aatggggaag
     3181 cgcatgtcac gctgaaaggt aaaaaagcgg gcacgcatac ggttaccgca acgctgggta
     3241 ataacaatac cagtgattcg cagccggtaa catttgtggc ggacaaagcc tcggctcagg
     3301 ttgtcctgca gatatcaaaa gatgagatca caggtaatgg cgtcgatagc gcaacgctaa
     3361 ctgcaacggt taaagatcag ttcgacaatg aggtgaataa tcttccggta acattcagct
     3421 cagcctcttc aggactcacc ctgaccccgg gagtgagtaa taccaatgag tctggcatcg
     3481 cgcaggccac tatcgcaggc gttgcctttg gtgagcagac ggtcacggca tcactggcta
     3541 ataatggtgc cagcgacaac aaaactgtgc attttattgg cgacacagcg gcggcaaaaa
     3601 ttatcgagtt gacgcctgtc ccagacagca taatcgccgg taccccgcag aacagcaccg
     3661 gcagcgtcat caccgccaca gtcgttgata ataatggctt tccggtgaaa ggtgtgactg
     3721 tgaacttcac cagccgaaca aactctgccg aaatgacgaa tggcggccaa gccgtaacga
     3781 acgaacaggg taaggcaacc gtcacttata ccaatacccg ctcctcgata gaatcaggag
     3841 cgagaccgga taccgttgag gccagtctgg aaaatggtaa ctccacgctt agcacatcaa
     3901 ttaatgtcaa cgcggatgcg tctacagcac atctcacctt gctacatgca ctttttgata
     3961 cagtctccgc aggcgagaca accagtctgt atattgaggt gaaggataat tacggcaatg
     4021 gtgtacccca gcatcaggta accctcagcg tttcaccaag tgaaggcgtg accctcagta
     4081 ataacggtat atatactacc aactactacg gctattttta cgcaagcttt accgctacaa
     4141 aagccggggt ttatcaagtg acggcaaccc tcgacaatgg cgattcgatg caacaaacag
     4201 tgacctatgt gccgaacgtc gcgaatgctg aaatcacgct ggcagcctcg aaggatccgg
     4261 tgattgccga caataacgat ctcacaacac taacagcaac agtcgctgat acagagggca
     4321 atgcgatagc caacactgag gtaacattta ctctgccgga agatgtgagg gcgaacttca
     4381 cgctgagcga tggcggtaaa gcgattactg atactgaagg caaagcgaaa gtcacgctga
     4441 aaggtataaa agcaggcgct catactgtta cagcatcgat ggctggcagt aagagtggac
     4501 aactggtggt gaactttact gcggatacgc tcactgcgca ggttaatctt aacgttaccg
     4561 aggacaattt catcgccaat aacattggga tgaccaagct gcaggcaaca gtgactgatg
     4621 gaaacggcaa cccattcgcc aatgaggcgg tgacattcac gctaccggca gatgtgagcg
     4681 caagcttcac tctcggacaa ggcggttccg ccattactga tatcaacggc aaggctgaag
     4741 ttacactgag cggtaccaaa tccggcacct atcccgtgac agttagcgtg atcaattatg
     4801 gtgtcagtga tacgaaacag gtgactttga ttgccgatgc tggtaccgca acactagcct
     4861 ccttaacctc tgtatactca ttcgtcgtta gcacgaccga gggcgcgacc atgactgcaa
     4921 gcgtcactga cgctaacggc aacccggtag aaggcataaa agttaatttc cgcggaacct
     4981 ccgtcacgct aagcagcacc agcgttgaaa cggatgatca gggtttcgct gaaattcttg
     5041 tgacaagcac cgaagtcgga ctgaaaacag tttcagcctc tctggcagat aaacctactg
     5101 aagtcatatc gcgattactg aatgcaaaag cagatattaa ttctgcaacg attaccagtc
     5161 tggagatacc tgaaggtcag ctaatggtcg cacaagacgt agcagttaaa gctcacgtca
     5221 acgaccagtt tggcaacccg attcttaatg aatctgtaac attcagtgca gagccaccag
     5281 agcacatgac catcagccaa aatattgtct ctactgatac gcatggtata gccgaggtct
     5341 ccatgacgcc cgaaagaaac ggttcgtata tggtgaaagc atccctggcg aatggagcct
     5401 cacttgagaa acaactggag gctattgatg aaaaactgac actcacggcg tccagtccgc
     5461 ttatcggtgt ctatgcccct acaggcacta ctctgacggc aacgctaacc tctgcaaatg
     5521 gcactccagt ggagggtcag gtcatcaact ttagcgtaac gccagaaggg gcgacgttaa
     5581 gtggcggaaa agtgagaact aactcttcag gtcaggctcc ggtcgttctg accagcaata
     5641 aagtcggtac atatacggtg actgcatcgt tccataacgg cgtaacaata cagacacaga
     5701 caaccgtgaa agtcactggc aactcaagca ccgcacatgt tgctagcttt atcgctgatc
     5761 catcgactat cgccgccacc aacagtgatt taagtacctt aaaggcaacg gttgaggatg
     5821 gcagtggtaa cctgatcgaa ggtctcactg tgtacttcgc cttaaaaagc ggctctgcca
     5881 cattaacgtc attaacagcg gtgaccgatc aaaacggaat cgcgacaaca agcgtgaaag
     5941 gagcgatgac aggtagcgtc acggtaagcg cagtcacgac cgctggtgga atgcaaacag
     6001 tagatataac gctggtggca ggcccggcag acgcctcgca gtccgtcctt aagaacaatc
     6061 ggtcctcact gaaaggggac tttaccgata gtgctgagct acatcttgtt ttgcacgata
     6121 tatcaggcaa tccgatcaaa gtttctgaag ggatggaatt tgtgcaatca ggtactaacg
     6181 tgccctatat gaaaattagc gcaattgatt acagtcaaaa tatcaacggt gattacaaag
     6241 ccaccattac aggcggcgga gagggtatcg caacgctgat ccctgtattg aatggtgttc
     6301 atcaagctgg tctgagtacc acaatacaat tcactcgcgc agaagacaaa ataatgagcg
     6361 gtacagtatc agtcaatggt actgacctac cgacaactac attcccttcg caggggttca
     6421 ccggggcgta ttatcagttg aataatgaca actttgcccc aggaaaaacg gcggctgatt
     6481 atgagttttc aagctctgcc tcctgggtcg atgttgatgc taccggtaaa gtgacattta
     6541 aaaatgtcgg cagcaattgg gaaaggatta cggcgacgcc aaaatcagga ggccctagct
     6601 atgtatacga aatccgtgtg aagagttggt gggtgaactc cggcgatgct ttcatgatat
     6661 acagccttgc tgaaaatttt tgcagtagca atggctacac acttccccgt gcagatcatt
     6721 taaaccatag tcgttcccga ggcatcgggt cactgtacag tgaatgggga gatatggggc
     6781 attacacgac tgaagctggt tttcaatcaa atatgtattg gtcatctagt cccgcaaact
     6841 caagcgaaca atacgtagtt tccctggcaa caggtgatca aagcgtattt gaaaagcttg
     6901 ggtttgctta tgcgacatgt tataaaaacc tgtgattttc cgttataatt tcttaaagag
     6961 tgctccattc ggagcactct tttttacatt ccctctatag agcttgccct tctctcacag
     7021 aaaatgaata aataaaactg ctgcaccgca agaatcgcgt tcgatgtgac tatgatcgta
     7081 tccggtgagt acagctttga tccaaaggct ggttttctgt gatgattgac cagcctcaga
     7141 gtattgatga caccaccgtt cataacagat cggcattact atgcataagt actgtctcat
     7201 attctcgcca aaacactttt taatgccttt gcgaattcat ccagacttat attgtcaatt
     7261 ttttgcccaa cgacgactga ctccgaaaca tcggccattg cgggccatcg cgttttgtaa
     7321 tcagcataaa ttgcaacaac gggagtatta taggcacttg ccatatgtaa tattgaggtg
     7381 tcaggagtta ttgcgatata agcatctttt atgattgctg ctgaacgttt aatcgagggt
     7441 gataaggata aacggtagac attattataa caatccacta ataccctggc tttatcttcc
     7501 ccttttggtc catgaacgat cactattggt atatctgttt cactttgaat ttttgctatt
     7561 agattttctg caatcgataa tgaaaatgta cgttcctgcg agctaccttc taaattaagc
     7621 gcaatgtaag atcctaacga tcgcatttcc tcgcgcacct catcgagtac atcctcactt
     7681 agtggcaatt catatattgg cctgactgct ggaaaacccg cctcacgcat cataaatgcc
     7741 catgtcatgg gtacaggagc ccggagattc tgatccatcc gggacgcgtt cttgcacaaa
     7801 ggggagtagc acttcatggt taaaccaaca acctgaaaat tcgtttttgc tttcaactga
     7861 ctgataaata tcatcgtttt caggttcttt ttacgcatcg cctctataca aagatccggc
     7921 gtaccgtatt tctgtgttat gttctttgct aaatctttta tttcttttaa tgttgcgtga
     7981 tcctgcatag tcattgtgac taatgttaat ttagtctttt caagtttaag tgcattaaac
     8041 acttctaaat taattgtcga cgtaacaatt aaaagatgct taattttatg caattcaagc
     8101 gcccgaataa caggaaagat ggccatagca tcgccaatct ggtcgggaat atggatgaca
     8161 acaaagtctg ttttttcaat attgaaatta taagctttat aatcgtagta actaaatgca
     8221 atacgtctca acaatgatgc taaaaacata cctaacctcg cctccctact ggttataatg
     8281 caatgcagtc tatcagactc atcagggtgc cattttgtgc atatgcggac ttttatgttt
     8341 catatctcta acctgtggct cctctgctta atccttaaac aacaccagca actcctgcgc
     8401 tttcatcttc catcgaattt ttcatgttgc cgctaatcag ccataaaatc atttgcagat
     8461 gcgctctgtc gaggtagtct cataaggttc gtttatagat cgacggcaat gtgagttacc
     8521 ttttccatac taattataaa aagacagtac aaacaggatc attatggact ccacgctcat
     8581 ctccactcgt cccgatgaag ggacgctttc gttaagtcgc gcccgacgag ctgcgttagg
     8641 cagcttcgct ggtgccgtcg tcgactggta tgatttttta ctctatggca tcaccgccgc
     8701 actggtgttt aatcgcgagt ttttcccgca agtaagcccg gcgatgggaa cgctcgccgc
     8761 atttgctacc tttggcgtcg gattcctttt ccgtccgctc ggcggtgtca ttttcggtca
     8821 ctttggcgac cgactgggac gtaagcgcat gttaatgctg accgtctgga tgatgggcat
     8881 cgcgacagcc ttgattggta ttcttccttc attctcgacc attgggtggt gggcacctat
     8941 tttactggtg acgctgcgtg ccattcaggg atttgccgtc ggcggcgaat ggggaggcgc
     9001 ggcgttgctt tccgttgaaa gtgcaccaaa aaataaaaaa gccttttaca gtagcggtgt
     9061 acaagttggc tacggtgtag gtttactgct ttcaaccgga ctggtttcat tgatcagtat
     9121 gatgacgact gacgaacagt ttttaagctg gggctggcgc attcctttcc tgtttagcat
     9181 cgtactggta ctgggagcat tgtgggtgcg caatggcatg gaggagtccg cggaatttga
     9241 acaacagcaa cattatcaag ctgccgcgaa aaaacgcatc ccggttatcg aagcgctgtt
     9301 acgacatccc ggtgctttcc tgaagattat tgcgctacga ctgtgcgaat tgctgacgat
     9361 gtacatcgtt actgcctttg cacttaatta ttcaacccag aatatggggt taccgcgcga
     9421 acttttcctc aatattggtt tgctggtagg tggattaagc tgcttgacaa ttccctgttt
     9481 tgcctggctt gccgatcgtt ttggtcgccg cagggtttat atcacaggtg cgttaatcgg
     9541 aacgttgagc gcatttcctt tctttatggc gcttgaagca caatctattt tctggatagt
     9601 tttcttctcc ataatgctgg caaacattgc gcatgacatg gtggtgtgtg tgcaacaacc
     9661 gatgtttacc gaaatgtttg gtgccagtta tcgctatagt ggtgctggag tcggttatca
     9721 ggtcgccagt gtggttggcg gtggatttac accttttatt gccgctgcac tcatcactta
     9781 ctttgccggg aactggcata gcgtcgccat ttatttgctg gctggatgtc tgatttccgc
     9841 aatgaccgct ttgttgatga aagacaatca acgcgcttga tagtctggcg aagatcatcc
     9901 gatcttcgcc ttacactttt gtttcacatt cctgtgacat actatcggat gtgcggtaat
     9961 tgtatggaac aggagacaca catgaataat aagggctccg gtctgacccc agctcaggca
    10021 ctggataaac tcgacgcgct gtatgagcaa tctgtagtcg cattacgcaa cgccattggc
    10081 aactatatta caagtggcga attacctgat gaaaacgccc gcaaacaagg tctttttgtc
    10141 tatccatcac tgaccgtaac ctgggacggt agcacaacca atccccccaa aacgcgcgca
    10201 tttggtcgtt ttactcacgc aggcagctac accaccacga ttactcgccc tactctcttt
    10261 cgttcgtatc tcaatgaaca acttacgttg ctgtatcagg attatggtgc gcatatctcg
    10321 gtgcaaccct cgcagcatga aatcccctat ccttatgtca tcgatggctc tgaattgaca
    10381 cttgatcgct caatgagcgc tgggttaact cgctacttcc cgacaacaga actggcgcaa
    10441 attggcgatg aaactgcaga cggcatttat catccaactg aattctcccc gctatcgcat
    10501 tttgatgcgc gccgcgtcga tttttccctc gcgcggttgc gccattatac cggtacgcca
    10561 gttgaacatt ttcagccgtt cgtcttgttt accaactaca cacgttatgt ggatgaattc
    10621 gttcgttggg gatgcagcca gatcctcgat cctgatagtc cctacattgc cctttcttgt
    10681 gctggcggga actggatcac cgccgaaacc gaagcgccag aagaagccat ttccgacctt
    10741 gcatggaaaa aacatcagat gccagcatgg catttaatta ccgccgatgg tcagggtatt
    10801 actctggtga atattggcgt gggaccgtca aatgctaaaa ccatctgcga tcatctggca
    10861 gtgctacgcc cggatgtctg gttgatgatt ggtcactgtg gcggattacg tgaaagtcag
    10921 gccattggcg attatgtact tgcacacgct tatttacgcg atgaccacgt tcttgatgcg
    10981 gttctgccgc ccgatattcc tattccgagc attgctgaag tgcaacgtgc gctttatgac
    11041 gccaccaagc tggtgagcgg caggcccggt gaggaagtca aacagcggct acgtactggt
    11101 actgtggtaa ccacagatga caggaactgg gaattacgtt actcagcttc tgcacttcgt
    11161 tttaacttaa gccgggccgt agcaattgat atggaaagtg caaccattgc cgcgcaagga
    11221 tatcgtttcc gcgtgccata cgggacacta ctgtgtgttt cagataaacc gttgcatggc
    11281 gagattaaac ttcccggcca ggctaaccgt ttttatgaag gcgctatttc cgaacatctg
    11341 caaattggca ttcgggcgat cgatttgctg cgcgcagaag gcgaccgact gcattcgcgt
    11401 aaattacgaa cctttaatga gccgccgttc cgataataag ttctgaacct tcttatcaga
    11461 aatagatgtg aaggagtaag taagacacct ggcaaatagc ctgcaataat cgtgggctgt
    11521 ttgcttcctt gggcggatac gtgttttatt atcgtcttaa tgattt
//
LOCUS       DAZFXT010000137        11463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.137, whole genome shotgun sequence.
ACCESSION   DAZFXT010000137 DAZFXT010000000
VERSION     DAZFXT010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.137"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            241..540
                     /gene="yoaC"
                     /locus_tag="ACHKW0_004033"
     CDS             241..540
                     /gene="yoaC"
                     /locus_tag="ACHKW0_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HFO7476780.1"
                     /translation="MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPA
                     SAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV"
     gene            complement(660..839)
                     /gene="yoaH"
                     /locus_tag="ACHKW0_004034"
     CDS             complement(660..839)
                     /gene="yoaH"
                     /locus_tag="ACHKW0_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HFO7476781.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            913..2274
                     /gene="pabB"
                     /locus_tag="ACHKW0_004035"
     CDS             913..2274
                     /gene="pabB"
                     /locus_tag="ACHKW0_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HFO7476782.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2278..2856
                     /gene="nudL"
                     /locus_tag="ACHKW0_004036"
     CDS             2278..2856
                     /gene="nudL"
                     /locus_tag="ACHKW0_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HFO7476783.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            3040..4404
                     /gene="sdaA"
                     /locus_tag="ACHKW0_004037"
     CDS             3040..4404
                     /gene="sdaA"
                     /locus_tag="ACHKW0_004037"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HFO7476784.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPLKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4535..6133
                     /gene="pdeD"
                     /locus_tag="ACHKW0_004038"
     CDS             4535..6133
                     /gene="pdeD"
                     /locus_tag="ACHKW0_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HFO7476785.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(6137..7693)
                     /gene="yoaE"
                     /locus_tag="ACHKW0_004039"
     CDS             complement(6137..7693)
                     /gene="yoaE"
                     /locus_tag="ACHKW0_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HFO7476786.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            complement(7681..7839)
                     /gene="yoaL"
                     /locus_tag="ACHKW0_004040"
     CDS             complement(7681..7839)
                     /gene="yoaL"
                     /locus_tag="ACHKW0_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaL"
                     /protein_id="HFO7476787.1"
                     /translation="MDRHRRHFSIRPFNACLSGSLCRTFRLHFVVTPALFLASNSYSL
                     SRSLSWNS"
     gene            8156..9127
                     /gene="manX"
                     /locus_tag="ACHKW0_004041"
     CDS             8156..9127
                     /gene="manX"
                     /locus_tag="ACHKW0_004041"
                     /EC_number="2.7.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HFO7476788.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            9190..9990
                     /gene="manY"
                     /locus_tag="ACHKW0_004042"
     CDS             9190..9990
                     /gene="manY"
                     /locus_tag="ACHKW0_004042"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HFO7476789.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            10003..10854
                     /gene="manZ"
                     /locus_tag="ACHKW0_004043"
     CDS             10003..10854
                     /gene="manZ"
                     /locus_tag="ACHKW0_004043"
                     /EC_number="2.7.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HFO7476790.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10909..11367
                     /gene="yobD"
                     /locus_tag="ACHKW0_004044"
     CDS             10909..11367
                     /gene="yobD"
                     /locus_tag="ACHKW0_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HFO7476791.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2802 a         2888 c         2880 g         2893 t
ORIGIN      
        1 tcgccctctg actagcaact ctggttttct ttcttagctg aataatctgg ttatagcgtg
       61 aggactgacc gttaaatttc tccatcttac tggataacac accatgagct gctaccttta
      121 aaacacctga atgtcaacag gttaactcgt gccatatcgt tataatccct ttgatattag
      181 atgcaaatta aggtcatata tagtcttatc gataacatgg ttaattttaa ggataagaat
      241 atgcctgctg taatagataa agccctggat ttcattggtg ccatggatgt atcagcgcca
      301 acaccaagtt cgatgaatga aagcacggcg aagggaatct ttaaatattt aaaagaactg
      361 ggagtacccg cgagtgccgc tgatattacg gcgcgagctg accaggaagg ctggaaccct
      421 gggttcacgg aaaaaatggt tggatgggca aaaaaaatgg agacaggtga acgctctgtg
      481 attaaaaatc ctgaatactt ttcaacatat atgcaggaag aactaaaagc actggtctga
      541 gttaaattta tatcagcata aatgggtcag gacgctttta atcacatata aaaagcgtcc
      601 tttttctcac aatcaaatga tcatcggctt ctataacgaa atcgatacgg attagtgtgt
      661 tactcgtctt catcctcaaa acgcgccacg attcgctcgc cggagtggtt ggcacgcagc
      721 tcttctgcca ccagcgcgat tgcctggccg ctgctcatcc cttgtgccat cagttcctgg
      781 atccgctcga cagctttttg ctgctgttca tgggtgagtg aaggtaaacc tgcaaacatt
      841 gttaactcct gctaaattgt tggcgctaat tatttcatgc tacccggcac atagccagta
      901 gagtcaggac tgatgaagac gttatctccc gctgtgatta ctttaccctg gcgtcaggac
      961 gccgctgaat tttatttctc ccgcttaagc cacctgccgt gggcgatgct tttacactcc
     1021 ggctatgccg atcatccgta tagccgcttt gatattgtgg tcgccgatcc gatttgcact
     1081 ttaaccactt tcggtaaaga aaccgttgtt agtgaaagcg aaaaacgcac aacgaccact
     1141 gatgacccgc tacaggtgct ccagcaggtg ctggatcgcg cagacattcg cccaacgcat
     1201 aacgaagatt tgccatttca gggcggcgca ctggggttgt ttggctacga tctgggccgc
     1261 cgttttgagt cactgccaga aattgcggaa caagatatcg ttctgccgga tatggcagtg
     1321 ggtatctacg attgggcgct cattgtcgac caccagcgtc atacagtttc tttgctgagt
     1381 cataatgatg tcaatgcccg tcgggcctgg ctggaaagcc agcaattctc gccgcaggaa
     1441 gatttcacgc tcacttccga ctggcaatcc aatatgaccc gcgagcagta cggcgaaaaa
     1501 tttcgccagg tacaggaata tctgcacagc ggtgattgct atcaggtgaa tctcgcccag
     1561 cgttttcatg cgacctattc tggcgatgaa tggcaggcat tccttcagct taatcaggcc
     1621 aaccgcgcgc catttagcgc ttttttacgt cttgaacagg gtgcaatttt aagcctttcg
     1681 ccagagcggt ttattctttg tgataatagt gaaatccaga cccgcccgat taaaggcacg
     1741 ctaccacgcc tgcccgatcc tcaggaagat agcaaacaag cagaaaaact ggcgaactca
     1801 gcgaaagatc gtgccgaaaa tctgatgatt gtcgatttaa tgcgtaatga tatcggtcgt
     1861 gttgccgtag ccggttcggt aaaagtacca gagctcttcg tggtggaacc cttccctgcc
     1921 gtgcatcatc tggtcagcac tataacggcg cgactaccag aacagttaca cgccagcgat
     1981 ctgctgcgcg cagcttttcc tggtggctca ataaccgggg ctccgaaagt acgggctatg
     2041 gaaattatcg acgaactgga accgcagcga cgtaatgcct ggtgcggcag cattggctat
     2101 ttgagctttt gcggcaacat ggataccagc attactatcc gcacgctgac tgccattaac
     2161 ggacaaatat actgctctgc gggcggtgga attgtcgccg atagccagga agaagcggaa
     2221 tatcaggaaa cttttgataa agttaataag atattacgcc aactggagaa gtaagacgtg
     2281 gaataccgta gcctgacgct tgatgatttt ttatctcgct ttcaactttt gcgcccgcaa
     2341 attaaccggg aaaccctaaa tcatcgtcag gctgccgtgt taatccccat cgtccgtcga
     2401 ccgcaaccgg ggttgttgct gactcagcgt tcaattcatc tgcgtaaaca cgctggacaa
     2461 gtggcattcc ctggaggtgc agtcgatgac acggacgcat cagttatcgc cgccgcgctg
     2521 cgcgaagctg aagaagaggt cgctataccg ccttccgccg ttgaagttat cggcgtgctg
     2581 ccgcccgtcg atagcgtaac tggctatcag gtaaccccag tggtcggcat tatcccgccc
     2641 gatctgccgt atcgcgccag tgaagatgaa gtctcggcgg tgtttgaaat gccgctcgcc
     2701 caggcattac atctgggtcg ttatcaccct ttagatatct accgccgtgg cgattcacat
     2761 cgggtatggc tgtcctggta cgaacagtat tttgtatggg gaatgaccgc aggcataatt
     2821 cgtgagctgg cgctgcaaat tggtgtgaaa ccctgactat acttatcttt acatctacaa
     2881 aacactactt gagacaatca tcgcaatatt agttaaatcg cggtttttga ttagtttaat
     2941 tcatgtgaat agttaagcca gtcgccgcgt tccctcttac actatgcgct gttattagtt
     3001 cgttactgga agtccagtca ccttgtcagg agtattatcg tgattagtct attcgacatg
     3061 tttaaggtgg ggattggtcc ctcatcttcc cataccgtag ggcctatgaa ggcgggtaaa
     3121 cagttcgtcg atgatctggt cgaaaaaggc ttactggata gcgttactcg cgttgccgtg
     3181 gacgtttatg gttcactgtc gctgacgggt aaaggccacc acaccgatat cgccattatt
     3241 atgggtcttg caggtaacga acctgccacc gtggatatcg acagtattcc cggttttatt
     3301 cgcgacgtag aagagcgcga acgtctgctg ctggcacagg gacggcatga agtggatttc
     3361 ccgcgcgaca acgggatgcg ttttcataac ggcaacctgc cgctgcatga aaacggtatg
     3421 caaatccacg cctataacgg cgatgaagtc gtctacagca aaacttatta ttccatcggc
     3481 ggcggtttta tcgtcgatga agaacacttt ggtcaggatg ctgccaacga agtgagcgtg
     3541 ccgtatccgc tcaaatctgc caccgaactg ctcgcgtact gtaatgaaac cggctattcg
     3601 ctgtctggtc tcgctatgca gaacgaactg gcgctgcaca gcaagaaaga gatcgacgag
     3661 tatttcgcgc atgtctggca aaccatgcag gcatgtatcg atcgcgggat gaacaccgaa
     3721 ggtgtactgc caggcccgct gcgcgtgcca cgtcgtgcgt ctgccctgcg ccggatgtta
     3781 gtttccagcg ataaactgtc taacgatccg atgaatgtca ttgactgggt aaacatgttt
     3841 gcgctggcag ttaacgaaga aaacgccgcc ggtggtcgtg tggtaactgc gccaaccaac
     3901 ggtgcctgcg gtatcgttcc ggcagtgctg gcttactatg accactttat tgaatcggtc
     3961 agcccggaca tctatacccg ttactttatg gcagcgggcg cgattggtgc attgtataaa
     4021 atgaacgcct ctatttccgg tgcggaagtt ggttgccagg gcgaagtggg tgttgcctgt
     4081 tcaatggctg ctgcgggtct tgcagaactg ctgggcggta gcccggaaca ggtttgcgtg
     4141 gcggcggaaa ttggcatgga acacaacctt ggtttaacct gcgacccggt tgcagggcag
     4201 gttcaggtgc cgtgcattga gcgtaatgcc attgcctctg tgaaggcgat taacgccgca
     4261 cggatggctc tgcgccgcac cagtgcaccg cgcgtctcgc tggataaggt catcgaaacg
     4321 atgtacgaaa ccggtaagga catgaacgcc aaataccgcg aaacctcacg cggtggtctg
     4381 gcaatcaaag tccagtgtga ctaatacttc ttactcgccc atctgcaacg gatgggcgaa
     4441 tttatacccg ctttctcgtc tgctgtaata ttccccacta cacttccact gttgcgtcag
     4501 gcgtttgtcg ccatacgctt acagggtggc ccgcatgcaa aaagcacaac ggatcattaa
     4561 aacctatcgc cgtaatcgaa tgattgtttg tacgatttgc gcactcgtta cgctcgcttc
     4621 gaccctgagc gtgcgattta tttcacagcg taacttaaat caacaacggg tagtacaatt
     4681 cgccaatcac gctgtagagg aattagataa agtactgctt cccctacagg caggtagcga
     4741 agtcttgctt ccgctgattg gtctgccctg ctctgtcgcc catttgccat tacgtaaaca
     4801 ggcggcaaaa ctccaaactg tgcgatccat tggcctggtg caagacggca cactttattg
     4861 ctccagcatt tttggttatc gcaatgtgcc cgtcgtggac attctggctg aacttcctgc
     4921 accgcaacca cttttacgcc tgacgatcga ccgtgccctg attaaaggca gtccggtttt
     4981 gattcaatgg acgcctgcag cgggcagtag caatgctggg gtcatggaga tgattaacat
     5041 cgacttactg acggcaatgc tccttgagcc acaactgccg caaatcagta gcgccagcct
     5101 gacggtggac aaacggcatt tgctctatgg taatgggctg gtagattccc ttccgcaacc
     5161 tgaagacaat gaaaactatc aggtttcttc gcaacgcttt ccttttacca ttaacgttaa
     5221 tggtccgggg gctacggcgc tggcatggca ctatcttcca acacaattac cgctggcggt
     5281 gctgctaagt ttactggtgg gctacattgc ctggctggcg accgcttacc ggatgagctt
     5341 ttcccgcgaa atcaatctgg gcctggcgca acatgagttc gaattgttct gtcagccttt
     5401 gcttaatgcg cgcagccagc aatgtattgg tgtagagatt ttgctgcgct ggaacaatcc
     5461 gcgtcagggc tggatttcac cggatgtgtt tattcctatc gcggaagaac atcatttaat
     5521 tgtgccactg acccgctatg tgatggcaga aaccattcgt cagcgccatg ttttcccgat
     5581 gagtagtcag tttcatgttg gcattaacgt cgcacccagc cattttcgcc gtggtgtgct
     5641 gataaaagat ctcaatcagt actggtttag cgctcacccg attcagcaac tgatcctcga
     5701 aatcaccgaa cgcgatgcct tactggatgt tgattatcgg attgcccgcg agctacatcg
     5761 taaaaacgtc aaactggcga ttgatgactt cggcaccggc aacagttcat tttcctggct
     5821 tgaaacatta cgtcctgacg tgctgaaaat tgataagtca tttaccgcag ctataggttc
     5881 tgacgcggtt aactcgacgg tgaccgatat catcatcgcg ctggggcaaa gactgaatat
     5941 tgaactggtg gcggagggcg tggaaacgca agaacaggcg aagtatttgc gccgtcatgg
     6001 cgtgcatatt ttgcaagggt atttgtacgc acagccgatg ccgctacgtg attttcccaa
     6061 atggctggcg ggcagccaac cgccgcccgc ccggcataat ggacatatca cgcccattat
     6121 gccgttacgt taacgcttac tcatcttcat cgtgcgccgg ttgctcttta acaatgcgaa
     6181 ccagatcaac acgataatca ttggcttcaa tgatggtgat atgcagtggc cctacatcaa
     6241 tcacatcgcc cacacgggga atgtgaccat ttgccgagat caccaggccc gcgaccgtcg
     6301 cgatatcatc gtcatcggca aggtgctcaa catcaagcgc ctgctgcaag gcatgcaaat
     6361 ctgtaccgcc ttttaccagc cagccgtcac catcggtaat gatttccggc gtttcgtcag
     6421 cgtccgggaa ttcacccgca atggcttcca gcacatccag cggcgtgacc aagccttgta
     6481 ccacaccaaa ctcgttggtc acgataacaa agctcccgcg agcgcgacgc agcacgccca
     6541 gcaggttgat cggatcgagg gtttccggga cgataatcgc cggagacgcc gaagcaatcg
     6601 ccgccacatc aacgccctct tccagcgcca ccagcagttc tttagcacgt acgattccga
     6661 tgatttcatc cagttcaccg cgacataccg ggaacaggct gtgcggtgag gagagcagct
     6721 gctcgcggat ttcatcgacc ccgagattag cgtcaaccca gcttatttca ccgcgcggcg
     6781 tcatgatccc gcgcagagaa cgcgacgcca gcgtcagtac gccgttaatc atgtaacgtt
     6841 cttcttcggc aaatgcacct tccgggatcg gcatcggcat cgggttatcg gcatcgtgct
     6901 gaacattggc ctgacgtttc ccgcccatca aacgcaggat ggcatcggca gtacgcgctc
     6961 gcagcggcaa agtcgactgg tgacgaataa agttgcgacg cgcaatctgg ttaaacactt
     7021 cgatgatgat cgagaagcca atcgcggcat acaggtaacc tttcggaatg tggaaaccga
     7081 aaccttctgc caccagactc agaccaatca ttaacaggaa gctcagacag agcaccacca
     7141 ccgtggggtg ctggttaacg aatcgcgtca gcggtttgga tgccagcaac ataaccgcca
     7201 tcgcaatcac taccgccgcc atcatcaccg gcagatggtt aaccatccct actgcagtaa
     7261 ttaccgcatc caacgagaag acggcgtcaa ggatgacaat ctgtgtgacg accacccaga
     7321 aactggcgta gcctttaccg tggccggaat catgatcgcg gttttccagc cgttcatgca
     7381 gttcggttgt tgctttgaac agcaagaata tccccccgaa caacataatc aggtcgcgtc
     7441 cggagaagga gaaatccatg acggtaaata gcggtttggt cagcgtgacc atccatgaaa
     7501 tcagcgacag cagccccaga cgcataatca gcgccagtga taaccccagc aaacgcgctt
     7561 tatcgcgttg ttttggcggc agtttgtcag caagaatggc gatgaagacc aggttatcga
     7621 tacccagcac aatttcgaga acaacaagcg tgagtagccc cgcccaaatt gaggggtcca
     7681 ttaagaattc catgacaagc tcctgcttaa ggaatagcta ttcgacgcca gaaataatgc
     7741 aggcgtaacg acaaaatgca aacgaaaggt gcggcataga gagccagaaa ggcaggcgtt
     7801 aaaaggcctg atgctgaaat gacgtcggtg acgatccata ctgcgggcta ctgccctata
     7861 ctccatggtt gttaaacggg agttaaacat atcagagacg cctctgattt ggcaaagatt
     7921 taccttcctt tgcaaacgaa tgtgacaagg atattttacc tttcgaaatt tctgctaatc
     7981 gaaagttaaa ttacggatct tcatcacata aaataatttt ttcgatatct aaaataaatc
     8041 gcgaaacgca ggggtttttg gttgtagccc ttatctgaat cgattcgatt gtggacgacg
     8101 attcaaaaat acatctggca cgttgaggtg ttaacgataa taaaggaggt agcaagtgac
     8161 cattgctatt gttataggca cacatggttg ggctgcagag cagttgctta aaacggcaga
     8221 aatgctgtta ggcgagcagg aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc
     8281 cgaaacgctg attgaaaagt acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt
     8341 gctgtttctc gttgatacat ggggaggcag cccgttcaat gctgccagcc gcattgtcgt
     8401 cgacaaagag cattatgaag tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt
     8461 aatggcccgt gatgatgacc caagctttga tgaactggtg gcactggcag tagaaacagg
     8521 ccgtgaaggc gtgaaagctc tgaaagccaa accggttgaa aaagccgcgc cagcacccgc
     8581 tgccgcagca ccaaaagcgg ctccaactcc ggcaaaacca atggggccaa acgactacat
     8641 ggttattggc cttgcgcgta tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg
     8701 gaccaaagaa accaatgtct cccgtattat tgttgttagt gatgaagtgg ctgcggatac
     8761 cgttcgtaag acactgctca cccaggttgc acctccgggc gtaacagcac acgtagttga
     8821 tgttgccaaa atgattcgcg tctacaacaa cccgaaatat gctggcgaac gcgtaatgct
     8881 gttatttacc aacccaacag atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc
     8941 tgttaacgtc ggtggtatgg cattccgtca gggtaaaacc caggtgaata acgcggtttc
     9001 ggttgatgaa aaagatatcg aggcgttcaa gaaactgaat gcgcgcggta ttgagctgga
     9061 agtccgtaag gtttccaccg atccgaaact gaaaatgatg gatctgatca gcaaaatcga
     9121 taagtaacgt attgtgttga ttatcactca gttttcacac ttaagtctta cgtaaacagg
     9181 agaagtacaa tggagattac cactcttcaa attgtgctgg tatttatcgt agcctgtatc
     9241 gcaggtatgg gatcaatcct cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc
     9301 ctggtgggta tcgttcttgg ggatatgaaa accggtatta ttatcggtgg tacgctggaa
     9361 atgatcgcgc tgggctggat gaacatcggt gctgcagttg cgcctgacgc cgctctggct
     9421 tctatcattt ctaccattct ggttatcgca ggtcatcaga gcattggtgc aggtatcgca
     9481 ctggcaatcc ctctggccgc tgcgggccag gtactgacca tcatcgttcg tactattacc
     9541 gttgctttcc agcacgctgc ggataaggct gctgataacg gcaacctgac agcgatttcc
     9601 tggatccacg tttcttctct gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc
     9661 gttgcgctgt ctgttggtac cagcgaagta cagaacatgc tgaatgcgat tccggaagtg
     9721 gtgaccaatg gtctgaatat cgccggtggc atgatcgtgg tggttggtta tgcgatggtt
     9781 atcaacatga tgcgtgctgg ctacctgatg ccgttcttct acctcggctt cgtaaccgca
     9841 gcattcacca actttaacct ggttgctctg ggtgtgattg gtactgttat ggcagtgctc
     9901 tacatccaac ttagcccgaa atacaaccgc gtagccggtg cgcctgctca ggcagctggt
     9961 aacaacgatc tcgataacga actggactaa caggtgagcg aaatggttga tacaactcaa
    10021 actaccaccg agaaaaaact cactcaaagt gatattcgtg gcgtcttcct gcgttctaac
    10081 ctcttccagg gttcatggaa cttcgaacgt atgcaggcac tgggtttctg cttctctatg
    10141 gtaccggcaa ttcgtcgcct ctaccctgag aacaacgaag ctcgtaaaca agctattcgc
    10201 cgtcacctgg agttctttaa cacccagccg ttcgtggctg cgccgattct cggcgtaacc
    10261 ctggcgctgg aagaacagcg tgctaatggc gcagagatcg acgacggtgc tatcaacggt
    10321 atcaaagtcg gtttgatggg gccgctggct ggtgtaggcg acccgatctt ctggggaacc
    10381 gtacgtcctg tatttgcagc gctgggtgct ggtatcgcga tgagcggcag cctgttaggt
    10441 ccgctgctgt tcttcatcct gtttaacctg gtgcgtctgg caacccgtta ctacggcgta
    10501 gcgtatggtt actccaaagg tatcgatatc gttaaagata tgggtggtgg cttcctgcaa
    10561 aaactgacgg aaggggcgtc tatcctcggc ctgtttgtca tgggggcatt ggttaacaag
    10621 tggacacatg tcaacatccc gctggttgtc tctcgcatta ctgaccagac gggcaaagaa
    10681 cacgttacta ctgtccagac tattctggac cagttaatgc caggcctggt accactgctg
    10741 ctgacctttg cttgtatgtg gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt
    10801 ggcttcttcg tcatcggtat cgctggttac gcttgcggcc tgctgggact gtaagactgt
    10861 tgtacactac cggggccttt tggccccgtt tttttatctg gaggattaat gacaatcacg
    10921 gacctggtac tgattctttt catcgccgca ctcctggcct tcgcgatcta cgatcagttc
    10981 atcatgcccc gccgtaacgg ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc
    11041 atcgatagcg ttatcttcgt cggattgatt gtcattctta tctataacaa cgtcacgaat
    11101 catggtgcgt taataacgac atggttatta agcgcactgg ctctgatggg tttttatata
    11161 ttctggatcc gcgttccgaa gatcatcttt aaacaaaaag gttttttctt cgccaatgtc
    11221 tggattgaat atagccgaat caaagcgatg aacttgtcgg aagatggcgt gttggtgatg
    11281 caattagaac agcgtcggct gttaatccgc gttcgaaata tcgacgatct ggaaaaaatt
    11341 tataagcttc tcgtttcaac tcaataagtt atgaatttag ccaaagctat gtttagtgta
    11401 tttttaataa tcagacatag cttaggctat attacctctt cccttatttg ttatttattt
    11461 taa
//
LOCUS       DAZFXT010000138        11412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.138, whole genome shotgun sequence.
ACCESSION   DAZFXT010000138 DAZFXT010000000
VERSION     DAZFXT010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.138"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..339)
                     /locus_tag="ACHKW0_004045"
                     /pseudo
     CDS             complement(1..339)
                     /locus_tag="ACHKW0_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347735.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(655..1299)
                     /locus_tag="ACHKW0_004046"
     CDS             complement(655..1299)
                     /locus_tag="ACHKW0_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476792.1"
                     /translation="MMKQGMANSISMTWQLLCDEHGFTREHYNQLKKFSPETLREIIA
                     EIASCHPSTSVLLRNKWLTPPEDILEQITREYERRIQNCPPFRSEKEAESWLNELSFA
                     VIAPLYRVAPEQTEQVESFVLQLIAEQERVWELILTNDGYSWHCALYDILFHLVLRNM
                     EDQPEHQKQRITKIFYEPDARLVAEEIKFRIQSLYDDEQKRALSELVNDFTSKE"
     gene            complement(1320..1589)
                     /locus_tag="ACHKW0_004047"
     CDS             complement(1320..1589)
                     /locus_tag="ACHKW0_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7476793.1"
                     /translation="MVAQKLEAAGCWRRASDRWLFVMGNVECTEAQREWLLLRRNYCL
                     AQISSPPLPETLDISEVAKAADATLRRMGIATPSGEVFRKGTPVC"
     gene            complement(1668..2306)
                     /locus_tag="ACHKW0_004048"
     CDS             complement(1668..2306)
                     /locus_tag="ACHKW0_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HFO7476794.1"
                     /translation="MGDSVTPEILGNMIRQYFSQERPEEEAIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMAPGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQVLGREAGTGKRLKGWLPVVCINPERKGQASRIAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSPAWTVR"
     gene            complement(2291..3522)
                     /locus_tag="ACHKW0_004049"
                     /pseudo
     CDS             complement(2291..3522)
                     /locus_tag="ACHKW0_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            complement(4023..6704)
                     /locus_tag="ACHKW0_004050"
     CDS             complement(4023..6704)
                     /locus_tag="ACHKW0_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HFO7476795.1"
                     /translation="MVTICVDGENKTHKITDWTLWAGNDDREVMLTCHFRSGRKYTRP
                     LSVCQITPTVNLRNVFLERKGNAVTSRAELVIIYGDKYAAVYYREGERPYIMKTTGLD
                     FQQCSAFTEHAVFNYLCRVANERIFYARGNNRNIDENILRQIKKIVPHPDTALHAYCS
                     GQSKKRDSPWGLIFPFGLNESQLLAVERAFSSQVSVIEGPPGTGKTQTILNIVANILI
                     QNKTVAILSNNNSAVSNVYEKMDKQQLGYVVARLGSTENRQQFFSTSISRSEEVLPDS
                     PSANAIDDVLQQVKKHLNAINQVASLKAEINELNIEYKYLQQWQSQNLRPEELFSHKY
                     RLSSRKTTDLMAYIHYLSDRRIGFRNRIDLLLNFRILKVKPLVIPERRLALFTSLQLS
                     YYEKFIREKQVSLNEYEEVLKESDFKILLERLTSWSVLYLKQHLRRNISTRNSFSAET
                     YRDEFNRFIKRFPVIGSSTHSIINSIGKGALLDYVIIDEASQQDIVPGILGLGCARNV
                     IVVGDRKQLPHVPVLLPNSPSPPAEYYNCEKYSLLDSVCMLFRNMVPVTLLKEHYRCH
                     PKIIQFCNKQFYDNALIPLTLDSGEASLSLIITAKGNHTRNFSNLRELESLEGHYWDE
                     ESSRGYIAPYNAQVNLAETVLPADFVKSTVHKFQGRECDEIVFSTVLDKKRSSQHSRN
                     IAFVDNPELVNVAVSRARNKFTLVTGNDVFERHAGHIAALIRYIKYYADDGEIFESPV
                     ISAFDLLYSEYDKSLERLNSRLNSNDSHFKSEQIVACLLRDILSQDSYRSMMFHSQIA
                     LNQLVLLERGDFTHREQLFMRNRASCDFVVYYKVGKTPLGVIEVDGGYHLTSVQAERD
                     ELKNSILKKCGLPLLRLRTIDSDIEGKLGAFLSGLTG"
     gene            complement(6945..7991)
                     /locus_tag="ACHKW0_004051"
     CDS             complement(6945..7991)
                     /locus_tag="ACHKW0_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HFO7476796.1"
                     /translation="MSALLSPLSLQAADVRRSGDEAFIIQQQRQEALEQQLTPSAPDV
                     RLSAPGSFAHKINFPVETPCFQIKQTELKGADALPHWLPLQKIANGAVGHCLGAKGIN
                     LLMSTLQNRLVDHGYVTTRVLAPSQDLKSGILRLVIIPGVVRHVRLTPDSDDYIQLYS
                     SFPAHEGSLLDLRDIEQGLDLGNSRIQGQHTELNATSGNLSTQNAQLSADTLSARTAG
                     QFSSNGGTINADTLQISAQSLSNRKGSLIQTGTGDFSLSLPGSVDNREGLLAANGAVR
                     LDALSLDNRKGKVQAEQSPSLQKSPPTFLKPFVAGVCAALLAVSVAIPGWQFLTQPSP
                     EEQHFTWGNGCKKQ"
     gene            complement(8095..8244)
                     /locus_tag="ACHKW0_004052"
     CDS             complement(8095..8244)
                     /locus_tag="ACHKW0_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476797.1"
                     /translation="MIKIKSILAGLLLSILVPVDAGKYRKGGSVPSQNMVGGIVRRLV
                     IVGMT"
     gene            8666..8857
                     /locus_tag="ACHKW0_004053"
     CDS             8666..8857
                     /locus_tag="ACHKW0_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7476798.1"
                     /translation="MSEDVPLPKVNQRYKDDHGALVTLTSVEETRVVFMQDGYPHPCM
                     RPMYNFLGKFKPEPREETE"
     gene            complement(8910..9143)
                     /locus_tag="ACHKW0_004054"
     CDS             complement(8910..9143)
                     /locus_tag="ACHKW0_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476799.1"
                     /translation="MARSDYDIINLSLEHELNEWLAERGYAGLVDNRNRLAEVVTRKL
                     QDSFYINVSRDALNTAYSEHPEWFSGLVSGDEN"
     gene            complement(9239..9862)
                     /locus_tag="ACHKW0_004055"
     CDS             complement(9239..9862)
                     /locus_tag="ACHKW0_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7476800.1"
                     /translation="MRTRIDYLADKYCFTERNESPRLRRQWQDVLEECRQTEAGPEER
                     LRIALLNVDYVTSFELPFRLLLTRTPQLIAALREEWGISQKNVVFNDKRFGCVYSLKA
                     SLSGVPDTYRYHLSHRIRRVVGNENTSLPYQQVAREVKAPRERLKYALEAGLLVTALD
                     GLFWFGSQRIAADVLRLRKAGMPVVTTTVEVHDNLTGTTRKVPAYHL"
     gene            complement(9951..10460)
                     /locus_tag="ACHKW0_004056"
     CDS             complement(9951..10460)
                     /locus_tag="ACHKW0_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476801.1"
                     /translation="MQITEALISEPGEIRRFVQQAVDHWPNLLAFHFTLYSAEGIYGQ
                     QIQTFCSSFHRRVHERITEHNHTVSPSAPVVLRWLREQHEGAQIRCLLLLSQTSICHP
                     RVGVMADEECAQLVDLLQQTWSVISAGGQCRVERCFRVARPGSSGQYVALKTAVQSFM
                     SQVIATIIR"
     gene            10918..11376
                     /locus_tag="ACHKW0_004057"
     CDS             10918..11376
                     /locus_tag="ACHKW0_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476802.1"
                     /translation="MKIYWTRKRIPELSALPPSLRKKNFTDAYNAASSHIEYWIGAGV
                     SFISMMILSRVYDFLLPAQDTFPGDIIRSLCVVCPSILIWFQFSVYVMRKYYRHILVR
                     GKETETISERLIREADTREYELWRPVRRFFSIVFLIVLLGCIHSLITTIK"
BASE COUNT         3178 a         2855 c         2290 g         3089 t
ORIGIN      
        1 acctgattct ggaaaaggac tttgtggatt gttgctattg ccagtgagat tgaatgaagt
       61 cgttttaact cgctttgaat acgtcttgcc cccagatttc gctgagaacg taactccaga
      121 atcagagcga cttcgcaagt gccggttttc gttgatggtg aacgcttggg gcgacggctg
      181 tggctttcca gaccagcgat gccctgagcc agatatctgc gccaccattt tacgcaatgt
      241 gggccgagaa atgccacagc gacggcatac aaatcccgca tcaccggacg tttcatagag
      301 ttttacccac tgtagccgct gctgaatttc cctgttcata atttggcatt agttcagctc
      361 aatcccgact taaaaagtcc ctacaggatg ttccggacct gaggcacagt tcatatcctt
      421 atccataaaa aatttagcta tgtctatagc attaatcttg acaagacgac gttagggcct
      481 ggcgatgaat catttcccct gccagatatc ctttggggtg caccagaact accgttttat
      541 atctatataa ctgaaacgtc cctcgccatc cttcatattt tgatgtagta acaccattca
      601 ctttcatcta ccccccgttt tattaacata tagtaaaaat cacctcgccg cattttattc
      661 ttttgaagta aaatcattta ccagttcact taaagctctc ttttgttcat catcatacag
      721 agattgaatc cggaatttaa tctcttctgc aaccagtctt gcatcgggct cataaaatat
      781 ttttgttatt cgctgtttct gatgttcagg ctggtcttcc atatttcgta aaaccagatg
      841 aaataatata tcataaagtg cgcaatgcca tgaataacca tcattggtta aaataagctc
      901 ccaaacccgc tcctgctcgg caataagctg cagaacaaag gactcaacct gttctgtttg
      961 ttcgggggca acacggtata gcggagcaat cacagcaaaa gatagttcat ttaaccagga
     1021 ttccgcttcc ttctcgctcc tgaacggtgg acagttttgt attcttcgtt cgtattcacg
     1081 ggtaatctgc tccagaatat cctccggcgg tgtcagccac ttattcctga gcaatacact
     1141 ggttgatgga tgacagctgg ctatttcagc aataatttca cgtaatgtct ccgggctgaa
     1201 cttcttcagc tgattgtagt gctcccgggt aaaaccatgt tcatcgcata aaagttgcca
     1261 tgtcattgat atactgtttg ccattccttg cttcatcatt aatacctgtt acctgaatat
     1321 caacagaccg gtgtaccttt tctgaatacc tccccggacg gagtggcaat ccccattcgc
     1381 ctcagcgtgg catctgcagc tttcgccacc tcgctgatat ccagcgtttc aggtagcggc
     1441 ggggacgata tctgcgccag acaataattc cggcgcaaca acaaccactc ccgttgtgct
     1501 tctgtacatt caacatttcc cataacaaaa agccagcggt cagaagctct gcgccagcaa
     1561 ccagcagcct ccagtttctg cgcaaccatg tcttccacaa cttccggggt actctttttc
     1621 ctgctcaaaa cattaactca ctgaaataat atacatatct gacggtctca gcgaaccgtc
     1681 catgccgggc tgaaatcctc ttcctgaaac agctccgtca gcccgctaat ctgtttcaat
     1741 ctgagaactt catcctggtc catacccagc tccgtaccaa tgcgttcatt cgtccagccc
     1801 aggcgcgaca ggtccctgac aatatcagac attgaggtta tctgatgttt gcccctggcc
     1861 cggttatgtc ggatggtcgc agcaatacgt gaggcctgtc cctttctctc cggattaata
     1921 cagaccaccg gcagccagcc cttcagacgt tttcccgtac ctgcttccct gcccagtacc
     1981 tggcgatgaa aaccatccac caccaagtaa tggttctttt cctcagacac taccaccggt
     2041 tgtgtgaaac catctttctc cagtgactgc ttcagcagtt tcttctcacc cggcgccatg
     2101 acattaggat tgtaatcatt ggccaccacc tcatctgctt ttacccacag cacacagtcc
     2161 acaggctcct gtgcaaaagg actgacctca tgaagcaccc tgcgcaggtg attaagggcc
     2221 tgtatggcct cttcttctgg tcgttcctgg ctgaagtact gacgaatcat gttgccaagt
     2281 atctccggtg tcacagaatc ccccattcct tcctcctttc cttcatacgc tgcaggtaac
     2341 gttcatagtg ccggggtttg ttagggctaa aggagagggt ccggcaccag aaatcattct
     2401 ttatgagagt cttacagata cgtcgccagg acggaatatc ccggctcccc aggtcattct
     2461 cctgttcatc cggaatgtca tccgggaagc cccgcgtctg ataccaacgc aggtagacag
     2521 caattttatt acggtaatgt tctgctgttc tttccggcat cacatccagc aggaacatcg
     2581 catagctgcg ccaggtatga tgaggcggct tcgtgatacg cttacacagg gcaaaatagg
     2641 caccactctc attggcataa agcgccccgc tggcagcccc tgacacccgt tcacacatcc
     2701 tggcccaggt ttccggctcc agaacatggt aaagccacag tcccttacgc tgttcaggac
     2761 caaaaggttc acagacacgc atgttgcgta acggcacgcc ggcacggtac atcaggtcat
     2821 acagaggatt gtaaatggca caggttctgg cgttatatat ccagatatcg cgggccttcc
     2881 agtcatacaa cggatacatg gtgtaataaa acccttcagg cgatgccgtg gtccagggct
     2941 tatcatcggc atatctcagt ttgcgctgag ataccagtcc cataaagcga ttgagcgatt
     3001 catcagcacg gacaccagtc agtattgcca ctccacaccg gttaccggca aaccaggaag
     3061 aaaatgccgg aacaaattct tcaaacgtca tggcataccg gtaaaaggga aaatacgtca
     3121 tgtctgtaat ggcctcttcc ggtggctggc gaacccaggt cactcctggc tcccagcata
     3181 tccactccgg ctgaaactgc gagacgccgt ttaccgtggt cagggaagtg ccacccagta
     3241 aaaggtttcc gtcacatcat ggtacatttc ccgcatcttc tgaatatgtt caatagtgca
     3301 ccgatactgg gcctcccagt caatgaacag aacagagaaa tgacgttttc tcctgcgggc
     3361 cacctctgcc acaagatgga aaagcacagt ggaatccttt ccacctgaaa aagacaggca
     3421 taccgacgga aatgtttcaa aaatccactc aatgcgggcg attgcggcac gcaaaacatt
     3481 ttctccggta aggatttttt cggttaaaag cgtatcactc attttcctgg ctttatgtcg
     3541 aataattctg tttatgcatt cagacaaact tcattaataa ctcaccgctt tggggataaa
     3601 taataacaat aaaactcttc agatattaat ctggttgttg gatttgttat cacatccttt
     3661 atccacctgc ttcttactgg taacacatct ggaaaatcca tgcattttca acatatacca
     3721 tcctttcaac ttgtaaataa ggaaatatga tgcagatggt gaacaattca aatatcattt
     3781 tgcaggaagt attcactgta atagaattac cccctgataa agtttattca ctaaggaatt
     3841 attcaacaga accattccct gttagctttg agcaatagca cttattttta tatgtatcag
     3901 gaatacgtac gttgatgtaa ccaaaacaaa gaagcctgtg tccatggggt tcgtacaatt
     3961 gtgtgtggca catgaccaaa tcaggtttga gacctcccgc tgcaatattt tttattcaga
     4021 ctttaacctg tcaggcctga cagaaacgct cccaacttcc cttctatatc actgtctata
     4081 gtacgtaatc ttagcaatgg caggccgcat ttcttcagta tactgttttt aagttcatca
     4141 cgttcagcct gaacactggt cagatgataa cctccatcca cttcaataac ccctaatgga
     4201 gtcttcccga ccttatagta aacaacaaag tcacaactgg cccggttacg catgaataac
     4261 tgttcacggt gtgtaaaatc cccccgctcc agcaacacca actgattcag cgctatttgt
     4321 gaatgaaaca tcatactccg ataactatcc tgcgacagga tatcccgcaa caggcaggcc
     4381 acaatctgtt ctgacttaaa gtgtgaatca tttgaattca atctactatt cagtcgttcc
     4441 agtgatttgt catactcact gtacagcaaa tcaaatgctg atatgaccgg gctctcgaaa
     4501 atctctccgt catcagcgta gtatttgatg taacgaatca gcgccgcaat atgtccagcg
     4561 tggcgttcaa atacatcatt accagtcacc agcgtaaatt tatttcgggc ccttgaaaca
     4621 gcgacattca ccagttcagg attatcaaca aacgcgatgt tccgactgtg ctggctgctg
     4681 cgcttcttat ccagcacagt ggaaaacaca atttcatcgc actcccgtcc ctgaaattta
     4741 tgcaccgtcg atttcacaaa atcagcaggc agcactgttt cagccagatt aacctgtgca
     4801 ttatatgggg cgatatatcc cctgctactt tcctcatccc agtaatgtcc ttccagagat
     4861 tcaagctccc tgagatttga aaaattacgg gtatggtttc cttttgccgt gatgatcaga
     4921 gataatgagg cttcacctga atcaagcgtt agcggtatca gagcattatc ataaaactgc
     4981 ttattacaaa attgaatgat tttaggatga cagcgataat gctctttaag aagagttact
     5041 ggcaccatat tcctgaacaa catacaaacc gaatcaagta gactatattt ctcacaatta
     5101 tagtattctg ccggtggaga tgggctgttc gggagtaata caggaacatg tggaagttgc
     5161 tttcgatccc ctacaacgat tacattacgg gcacatccca gtcccagaat gcccggaaca
     5221 atatcctgct gtgaggcttc atcaataatc acataatcaa gaagagcccc cttaccgata
     5281 gagttaataa tagaatgcgt gctgctacca ataaccggaa aacgctttat aaaacggtta
     5341 aattcatccc tgtatgtctc tgccgagaat gaatttcttg ttgaaatatt tcttcgaaga
     5401 tgttgtttta aataaagcac agaccatgat gtcaggcgct ccagtaaaat tttaaaatca
     5461 gattccttta atacttcctc atattcattc agagaaactt gtttttcccg gatgaatttt
     5521 tcataataag aaagctgaag agaagtaaat agtgccagcc ttctctccgg aatcaccaat
     5581 ggtttgactt tcagtatcct gaagtttaac aacaaatcaa ttctgtttct gaatccaatc
     5641 cgcctgtcag aaaggtaatg aatataagcc atcaggtcag ttgtttttcg tgaggaaagt
     5701 cggtatttgt gagaaaaaag ctcctccggt cgaagattct gactctgcca ttgctgcagg
     5761 tatttatact caatattcag ctcattaatt tctgctttta acgaagcgac ctggttaatg
     5821 gcattcaggt gttttttcac ttgttgaagt acatcatcaa tcgcatttgc agacggtgag
     5881 tcaggtaaga cttcctcaga acgcgaaatc gacgttgaaa aaaactgttg cctgttctcc
     5941 gtactcccca gtcgtgccac cacatatccc agttgctgtt tgtccatctt ttcataaaca
     6001 ttactgacag ctgagttatt attggacaaa attgccactg ttttattctg aattaatata
     6061 ttagcaacaa tattgagtat agtctgtgtt ttacctgttc caggtggtcc ttcaataact
     6121 gaaacctgtg aggaaaatgc ccgttcgaca gccagcaact gactttcatt aagaccaaac
     6181 ggaaaaatca aaccccatgg cgaatcacgt tttttactct gtcctgaaca gtatgcatgc
     6241 aaggcggtat ccggatgagg aactattttc tttatctgcc taaggatatt ctcatcgata
     6301 tttctgttat taccgcgggc ataaaaaata cgttcattag ccaccctaca caagtaatta
     6361 aacacggcat gttctgtaaa tgctgaacac tgttgaaagt caagaccggt cgttttcatg
     6421 atgtagggtc gttcaccctc gcgataataa acagcagcat atttatcacc atatataata
     6481 accagttcag cccgggatgt tacagcgttc cctttccttt cgaggaaaac atttcgcaaa
     6541 tttacagttg gcgttatctg acatacagaa agcggacgag tatattttct cccgctgcga
     6601 aaatgacagg tcagcataac ctctctgtcg tcatttccag cccacagcgt ccagtcggta
     6661 attttatgag tcttattttc accgtcaaca caaatcgtga ccatattctt actcagaaat
     6721 tatcatttaa cttacggaaa attgttaagg cactcccgat attagctccg gaggattttc
     6781 cacaactccg ggactgttaa catatttctg cctgccatat atacattgaa tttagtcata
     6841 acagtcaata ccagaattac aggaatgcga tgccgatacg ctggttattg aatactggta
     6901 gcccctgagc actaattcat cacgatactc tgtatttact gttttcactg ctttttacaa
     6961 ccattccccc aggtaaaatg ctgctcctcc ggtgatggct gtgtcagaaa ctgccatccc
     7021 ggaatagcca cgctgaccgc cagcaatgcc gcacagacac cagccacaaa cggtttcaga
     7081 aacgtgggcg gggatttctg aagggagggt gactgctccg cctgcacttt ccccttgcga
     7141 ttatcaaggc tcagtgcatc cagacgcacc gcgccatttg ccgcaagcag cccttcccgg
     7201 ttatccacgc ttcccggcag actcagcgaa aaatcccctg ttcccgtctg aatcagactg
     7261 cctttacgat ttgacaggct ttgtgccgat atctgcagtg tgtcggcatt tatcgtaccg
     7321 ccattactgc tgaactgccc ggcagtccgg gcggaaagcg tatcggcact cagttgcgca
     7381 ttctgtgtag acagatttcc actggttgca ttcagctcag tatgttgtcc ctgtatccgg
     7441 ctgttaccta aatccagccc ctgctcaatg tcccgtaaat ccagcagaga accttcgtgt
     7501 gccgggaatg aggaatacaa ctgaatatag tcatcactgt ccggtgtcag acgcacatgt
     7561 cgcacaacac cgggaataat aaccagccgg aggataccgc tttttaaatc ctgcgaaggt
     7621 gccagaacac gggtggtgac ataaccatga tcgaccagac ggttctgcaa tgtactcatc
     7681 agcagattaa ttcctttcgc ccccaggcaa tgcccgaccg ccccgttggc gattttttgt
     7741 aaaggcagcc agtgtggtaa cgcatcagcc cccttcagtt ccgtctgttt aatctgaaaa
     7801 cagggcgttt caacaggaaa attaatctta tgggcaaaag agccaggtgc agaaaggcga
     7861 acatcagggg ctgaaggcgt cagttgttgc tcaagggctt cctgacgctg ctgctgaatg
     7921 ataaatgctt catctccgct acgccggaca tccgctgcct gaagagataa aggcgataat
     7981 aaagcagaca tcagtgtcag tgacagcaag gacggacagc gccggaataa gctttgttcc
     8041 tcagtcctct cgttcctgca acataatttt ccagtcaaac gaaaaacctt ttattcatgt
     8101 cataccaaca atcaccagcc tcctgacgat acctcccacc atgttttgcg atggcacgct
     8161 accgcccttc cggtatttac ccgcatcaac cggtaccaga atgctgagta acaaaccagc
     8221 tagaattgac ttaattttga tcattataga ataatgatct gaactacagc aacatgacag
     8281 atttatcgat cgatataaca caaatgaata gttctttatt ctgacaaata tcgcaaaaac
     8341 agcacattac aaaatttata accataatta ctggaaaagt gcagaaaatt attccactga
     8401 atgcttttaa tacagttggt tagcttaaaa aatagcaatt tttgatttca gtcattgaaa
     8461 gttgcaatgt ttgacgattc aacagcattt aagtgagttt caggatatgg gaaggacatg
     8521 aaaggaggag cgcaataaaa tacacaggaa gaaggggaaa atgtttcgta aaaacagaaa
     8581 attaacagcg cacatcagga aacgcagtct cctgattcag tgctaccaca tgaaaattta
     8641 gttaactgtt accgtactaa aatttatgtc agaagatgtt ccattaccga aagtcaatca
     8701 gcgatacaaa gacgatcatg gtgcgcttgt caccctgacc agtgttgagg aaacccgggt
     8761 ggtgtttatg caggacggct atccgcatcc ctgcatgcga ccgatgtaca actttctggg
     8821 aaaattcaaa cccgaacccc gggaagaaac agaataaaac gaaatccctg ccggcaatca
     8881 cactacctga tagcagcata atccacttat taattctcat ccccggagac aagcccagaa
     8941 aaccactccg ggtgttcact gtatgccgtg ttcagcgcat ccctggaaac gtttatataa
     9001 aagctgtcct gcaatttgcg ggtaacaact tctgccaggc gattccggtt atccaccagt
     9061 ccggcataac ctctctctgc cagccactca ttcagttcat gttccagaga cagattgata
     9121 atatcataat cggaccttgc cattcccgtt tccttagcac atgttttcag gcacggggat
     9181 aaagtataaa gaatgtcata ctgctgctgc cagagcaata ttccccctga tggttgtatc
     9241 agagatggta tgccggtact ttgcgggttg ttcccgtcag gttatcgtgc acctccacgg
     9301 tcgtggtcac caccggcatt ccggcctttc tcagcctcag aacatcagcc gcaatacgct
     9361 gactgccaaa ccagaacagg ccgtccagtg cagtcaccag taaacccgcc tccagtgcat
     9421 acttcagccg ttcacggggc gctttcactt cccgggcaac ctgctgatat ggcaatgatg
     9481 tgttttcatt cccaaccacg cgacgaatac ggtgagacag atgataccgg tatgtatccg
     9541 gcacaccaga aaggctggcc ttcaggctgt acacgcagcc aaaccgttta tcgttaaaca
     9601 ccacattttt ctggctgatg ccccattctt cacgcagcgc ggcaatcaat tgcggtgtac
     9661 gggtaagcag caagcgaaaa ggcagttcaa aactggtgac gtaatccaca ttcagcagtg
     9721 caatacgcaa ccgttcttct ggcccggcct ctgtctgccg gcactcctcc agaacatcct
     9781 gccactgccg gcgaagacgg ggggattcgt tccgttctgt aaaacagtat ttatccgcca
     9841 gatagtcaat tcgtgtacgc atactgaaag gaattccgta taaagattca gcagacaaaa
     9901 ctttatcagc cagtaaaaat taacggaagc attgatattc ctcccgacaa tcaccggatg
     9961 attgttgcaa taacctgcga cataaaggac tgaacagccg tttttaacgc cacgtattgc
    10021 cctgatgaac caggccgggc cacccgaaaa catctttcca cccggcattg tccgccagca
    10081 cttatcaccg accaggtctg ttgcagtaaa tccaccagtt gcgcacattc ttcatcagcc
    10141 atgacgccga ctcgcggatg gcaaatactc gtctggctga gcagcaacag acatcgtatc
    10201 tgtgctcctt catgttgttc ccgcaaccag cgtaatacca ccggcgctga tgggctgacc
    10261 gtgtgattat gctccgtaat acgttcgtgt acccgtcggt gaaaggaact acaaaatgtc
    10321 tgaatctgtt gaccatagat tccttctgcc gaatagagcg tgaagtgaaa tgccaacaga
    10381 tttggccagt ggtccaccgc ctgctgaaca aaacgccgga tttcccccgg ctctgaaatt
    10441 aaggcttcgg ttatttgcac gaaatgttcc ttttcaaccc ctgttgaatt ttgtcaagcc
    10501 ggtgcagaca cagtaaagcg cgcatcaaca cctgaaaggt acgatacaaa cacaagttac
    10561 aaacaataag aacggaagga caattaatca gtgaggaata ataactaaat taaccaaaag
    10621 aatgttactg acaatactac caatacttaa atagcaatat attaattaat gtaagaacag
    10681 atgcccggta ggacatatac acaatacatc cacaaacaca ctcttttcat taaatcaatc
    10741 acctgattaa accatgaaag tgtgatgtaa gtcaaaatta ctgatactat caattgataa
    10801 tgattatctc tatcattatc gtgaaattcg agaatgaaag gagaagtagt cattataagc
    10861 cttttataaa tcgtcatcat catagtaaag tagaacactt tttatatgag ttactatatg
    10921 aaaatatact ggacaagaaa acgtattcct gaactttcag cacttccacc ttcattaaga
    10981 aagaaaaatt ttactgacgc atataatgca gcatcttctc atatcgaata ctggataggt
    11041 gctggcgtct catttatctc gatgatgatt ttatccagag tatatgactt tcttttaccc
    11101 gcccaagata ctttcccggg tgatatcatc agaagtttat gtgttgtgtg tccatccata
    11161 ttaatctggt ttcagttttc ggtatatgtc atgaggaaat attacagaca tattctggtg
    11221 cgaggaaaag aaaccgaaac catttcagaa aggttgatac gtgaggctga tacacgagaa
    11281 tatgaactat ggcgtccggt ccgtcgcttt ttctccattg ttttcctcat tgttctatta
    11341 ggttgcattc attcactgat aacaactata aaataaaagt tatggctgtt ctgatacagg
    11401 aaaataaacg tt
//
LOCUS       DAZFXT010000139        11366 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.139, whole genome shotgun sequence.
ACCESSION   DAZFXT010000139 DAZFXT010000000
VERSION     DAZFXT010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11366
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.139"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..842
                     /locus_tag="ACHKW0_004058"
     CDS             <1..842
                     /locus_tag="ACHKW0_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin co-regulated pilus biosynthesis Q family
                     protein"
                     /protein_id="HFO7476803.1"
                     /translation="AVSSQSTALPPRPLLQATQLPALVMTGKPGQSSVVYLREARQRS
                     VSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHRALNTLLTGQQLYGHMDWN
                     RKILTVTTTATPPVDLTAPQGSQKTADTPRNPFRSTTGTAADQAQVPPAVSASRVQST
                     TGTSGTSAPRTTTPAPVVTKPVAPPQKVTPPRQWKMEKGTTLKDGLARWTSSEKCSHG
                     TWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPVEKPLYARTSTAQCLLRVTDR
                     PQE"
     gene            847..1284
                     /gene="pilM"
                     /locus_tag="ACHKW0_004059"
     CDS             847..1284
                     /gene="pilM"
                     /locus_tag="ACHKW0_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HFO7476804.1"
                     /translation="MGWFIVFIMVITTTGNFWFTSQLSRSEHRHQAAENTQQAATFIR
                     YMNAINDYLHQHPERRTAGGRLTSAQLGIPGTDTVSHIISQQRVFVWATETPGLMAAL
                     REQSNDSALLGRVENGRLLDTAGRALSVTLPSVIPDHVILWMN"
     gene            1315..2934
                     /locus_tag="ACHKW0_004060"
     CDS             1315..2934
                     /locus_tag="ACHKW0_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HFO7476805.1"
                     /translation="MNKKILAIMIAAALLQGCASYRQLESTRKEAVAEQQRAREMMSD
                     LRTRTPVVKESSRQWINPRPVMDKNTGKTVPGCPIVIHTRQSITLQQVVQRITESCHI
                     PVRITPDVLTYLNTSTTGSTQQIQGALPPPDASGMVPLAAIGASDTPPSHLPGNTAAL
                     SELNASGLTTLLNAVSSRLGISWRYDGSHIIFYYLETQTFPITYMDSQVAYNATVVSG
                     TMSSNGSSGGSASDAISGDASNTQTTTVDMKSALYQDVKNAVTSMLTPGIGRMFMSTG
                     FITVTDNPQVLETVRAFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDAITGGVSIVDGKLTGSKAFLKALSSQGDVSVVTQNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            2955..4250
                     /gene="pilO2"
                     /locus_tag="ACHKW0_004061"
     CDS             2955..4250
                     /gene="pilO2"
                     /locus_tag="ACHKW0_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HFO7476806.1"
                     /translation="MTDISPTGRQVFPLTAGRNVFLAGLEWKTLPSQYRHARDFARAQ
                     KADLFLACQYLSNEDADTHTMVATVSRRILPGKPRQCFSLALLILPLLEHGGYAITEL
                     TLPGETPHYSFVSVVDGVLVSDLVGSGEEVREARDTFLSINTEPTQGWIHYAPAAFSA
                     GDQNQHLPLASLTGSGKHPAAARLNPVSRGPQFITVSLIIALLGTAWYGWQYYERWQT
                     EKAAQAAVARQATEERITPPWPGLPETDAFIRGCADIWTSLPLSAAGWRYSLAECSTD
                     GGSGNLRASYTNTAGATVTDFIRRISDMTDSRPFIVMPEGKTGGIGLPVTFRLSENAV
                     TVDALPETAVLQERLTSLAQSMQLKLDWQEVNNSLTDENGQIIQPPWKEYDLQMQTTL
                     PAHHLAERLKEPSVRFISVTRQLENGRFHYQFTGKYYAR"
     gene            4240..4692
                     /gene="pilP"
                     /locus_tag="ACHKW0_004062"
     CDS             4240..4692
                     /gene="pilP"
                     /locus_tag="ACHKW0_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HFO7476807.1"
                     /translation="MPDKAKTLLFSVLLLPATAMATTGNAEQQNTQQIRIEQIQEETA
                     LYEAMLAREKIRHELRTLEQGVPAEAGRQQESISAVTTNTQASLSPRLTAIFGVQPQL
                     TARIELPGGSISDVKAGQNIPGTTYHVEKITPDSVVLEQSGTRKTLRP"
     gene            4777..6285
                     /locus_tag="ACHKW0_004063"
     CDS             4777..6285
                     /locus_tag="ACHKW0_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HFO7476808.1"
                     /translation="MLDPADLPPDDILDYVAIDTDNAGQLRIRVVEGKKHLRAVQEYL
                     TRLRARYQGRVGDFEFTTLDVIARIREATTIAGQENGMNPVQNKMLGYIRHAATLNAS
                     DLHITPGRDNTDFTYVEARVHGELEILDIIRKDEGVELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAEKFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSSHIPTFGMLGYHPKQE
                     QTIRRILQRPEGIITLSGPTGSGKSTTLRTASAAYLEQHGFNNTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDSAQGWVDSIKSALRLDPDGILNGEIRDHDSAITAIKAAMTG
                     HLMLTTIHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQLICPHCRHPWNDV
                     ASRRTEDERRLVETFCNADAVYLRNHDGCPHCWRGVTGRTVIAEVISPDARFFQLYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGLVDPLAAHYISPIDEDSYTLLR"
     gene            6287..7387
                     /locus_tag="ACHKW0_004064"
     CDS             6287..7387
                     /locus_tag="ACHKW0_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000670135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HFO7476809.1"
                     /translation="MKFLEKLNSTELDLSALNSWTARKTFGLKRAEFYESLGGMIRDG
                     TPSLRALEFICNVETDFGTRKGTSGMYFLARECIASLKASGMLSGALPTWVPPEEAAL
                     IRNGEERGNIAEALFQVAKTVKARREMVSSLITLCLYPLLLLSLCVVNMYNAHTRIIP
                     IVSAFAPEEKWSFQMKVLAAMSELVIGYGLWLSGAIITLAIIIRFSLSRYTGPGRRLL
                     DRLPPWSLYQTFHGVNYLFSISSMLQINIPLSQALLRIEKSAEHNKWLKERIQAIRKN
                     VLSGQNLATAMRNSDYDFPSKICINKLLLNSEREDSVSAMNTYAGQWLEDAKKSVRKS
                     GLIITGISGGFVFFFILNMISAIYSISDMLKQ"
     gene            7461..7997
                     /locus_tag="ACHKW0_004065"
     CDS             7461..7997
                     /locus_tag="ACHKW0_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009447271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4 pilus major pilin"
                     /protein_id="HFO7476810.1"
                     /translation="MNFRARLSSYFSLKKDKEEDKGLTLLEIAGAMIIIAIVVALAMS
                     QMSSTMASSDNQRELKNLQTIATKMKQYKFQGRYTGTDYIKTLTESGALPADMIAGGN
                     KAKNAWGGDVTIAATADKYGYIITSNNVPKTNCVELINSLRSSSIFTKIMNTAPNMVD
                     PVTVCSSDKNNIKLETNS"
     gene            8051..8524
                     /locus_tag="ACHKW0_004066"
     CDS             8051..8524
                     /locus_tag="ACHKW0_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HFO7476811.1"
                     /translation="MKWFYLSSLFLFCLPATAAVNSNTLDSCFIQAGKRYSIDPGLLK
                     VIARKESSLNPRAINHNRNASGKIISTDYGLMQINSTHIPGLKRMGIIRSENELLSNV
                     CLNIQTGAWILARHFQQCGVNWECLGSYNAGFNKNNTHRRMKYARLIYAAYMQGR"
     gene            8543..9178
                     /locus_tag="ACHKW0_004067"
     CDS             8543..9178
                     /locus_tag="ACHKW0_004067"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HFO7476812.1"
                     /translation="MFLIILLVISATLTIQLLAIRLAGHYLMQVHTLRRPRHLSLLCA
                     VLALTATITHIIRGTDIAGLCFLLLMAGCMTLMTLTDAASCRLPRPFTLTFLMLGVAF
                     RLWQGDAAIALACALFWFLLLSLLRRYTSYRKGVETLGLGDVFLIAGVAMWSSANSTP
                     WIIGIAAASTMICMSLFPRSNTGAAIPFGPFICASLYIFTFFPGAIFHTGG"
     gene            9183..10547
                     /gene="pilV"
                     /locus_tag="ACHKW0_004068"
     CDS             9183..10547
                     /gene="pilV"
                     /locus_tag="ACHKW0_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HFO7476813.1"
                     /translation="MQKDDDKGFVLFEILAGLIVIGIATPMIYSEIENWLNEQLYQSA
                     AYHADAYNTAARNYIADNNVRLHSGSLPANFTADDLIRQGYLKQGFNHSPFGQSYITG
                     IRRNQTTGRLEALTCSTGGQTIKEEGLRSVAGQLPGLGGFISKNGTATGAFGAWTDKP
                     GDYGLTCSTGHIAVVMSGDDLQESDRLYRFQVAGRPELNQMHTAINMGGNNINNTGNI
                     NGQSATLKGDITSEDGWLITRNNKGWMNITHGGGFTMTDSQWIRAVNGKGITTTGEIK
                     GGKVSGGTVRSDGRLSTGEYLQLDKTATAGTKCAPDGLVGRTSTGAILSCQSGMWAGF
                     ESYPVGSPIPWPSATPPQGYLVMNGQSFSCSRYPQLARAYPGCKLPDLRGVFIRGWDN
                     GRGLDGDRNRHLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYI
                     VKAS"
     gene            10805..10963
                     /locus_tag="ACHKW0_004069"
                     /pseudo
     CDS             10805..10963
                     /locus_tag="ACHKW0_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000486835.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            11005..11295
                     /locus_tag="ACHKW0_004070"
     CDS             11005..11295
                     /locus_tag="ACHKW0_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476814.1"
                     /translation="MKHLSSRAATADNNRIVTIEDVYTIAFSQPIHTESALGQRWMLI
                     HTGEYESASDAKRFLDTLVTGHCACMVTPSPVLDHDTWLRIRLLMGEIRDAK"
BASE COUNT         2905 a         3129 c         2831 g         2501 t
ORIGIN      
        1 ccgcagtcag cagccagagt accgcactgc ctccccgccc gctccttcag gccacacagc
       61 tgcccgcact ggttatgacc ggtaaacccg gtcagtcatc cgtcgtttat ttacgtgagg
      121 cacgtcagcg ctccgtcagt gactggatac gccagttgct accacccggc tggcagttcc
      181 ggcaacagaa cgctgccata ccggcactga acaaacgcct ccccgtctgg tccgttaatg
      241 accagtggca ccgggcactg aatactctgc tgaccgggca gcaactgtac ggacatatgg
      301 actggaacag gaaaatactg actgtcacga ccactgcaac accgcctgtt gacctcacgg
      361 caccgcaggg ttcacagaaa acagcggaca caccacgtaa tccgttcagg agcacaaccg
      421 gaacggctgc agaccaggca caggtaccac ctgccgtcag cgcttcccgg gttcagagca
      481 caacagggac atccggtaca tctgcgcccc gcacaaccac gccagccccg gtggtcacga
      541 aaccggtagc accaccacag aaggtcacac cgccacggca gtggaaaatg gaaaaaggga
      601 ccacgctgaa ggacggactg gcccgctgga cgtcgtccga aaaatgctcc cacggcacct
      661 ggacgctgcg ctgggaaacg cccgttaact acagtatcga tgcccctctg gttttcaccg
      721 gcagtttccg ccaggcgctg gaacagctct tcgcactcta caccccggtg gaaaaaccgc
      781 tgtatgcccg gacaagcaca gcacaatgtc tgctgcgggt gacagatcgt ccacaggaat
      841 aaccttatgg gctggttcat cgtgttcatc atggtcatca cgaccacagg caatttctgg
      901 ttcaccagtc agctctcccg cagcgaacac cgtcatcagg ccgcagaaaa cacacaacag
      961 gcagcaacgt tcattcgtta tatgaacgcc atcaatgatt acctgcatca gcatccggaa
     1021 cgacggacag ccggagggcg gctgacatcc gctcagctcg ggattcccgg gacggatacc
     1081 gtcagtcata tcatcagcca gcaacgtgtt tttgtctggg ctacagaaac cccgggcctg
     1141 atggccgcgc tcagggaaca gagtaatgac tctgctctgc tcggcagggt ggaaaacggc
     1201 cgactgctgg acacagccgg cagagcgctt tccgtcacac ttccgtcagt gatccccgat
     1261 cacgtcatcc tctggatgaa ttagtcacga atctgaataa atcacggatc tcccatgaat
     1321 aaaaaaattc ttgccattat gattgccgcc gctctgttac agggctgtgc cagttacaga
     1381 cagcttgaga gcacaagaaa ggaagccgtt gcggaacaac aacgggcacg ggaaatgatg
     1441 tctgatctgc gaacccggac gccggttgtt aaggaatcct cacgacaatg gatcaatccc
     1501 cgtccggtca tggataaaaa caccggcaaa accgttcccg gttgtcccat tgtcattcat
     1561 acccgtcaga gcatcaccct tcagcaggtg gttcagcgga tcacggaaag ctgccatatt
     1621 ccggtacgga tcacacctga tgtactgaca tacctgaata catccacaac cggcagcaca
     1681 caacaaatcc agggcgcatt acccccaccg gatgcgagcg gcatggttcc ccttgccgcc
     1741 attggcgcat ctgacacgcc accatcccat ctgccgggaa ataccgcagc actgtctgaa
     1801 ctgaatgcct ccggcctgac gaccctgctg aatgcggtct cctcccgtct cggtatatcc
     1861 tggcgctatg acggctcgca tatcatcttc tattatctcg agacacagac cttccccatc
     1921 acctatatgg acagtcaggt ggcctataac gccactgtgg tcagcggcac catgtcctca
     1981 aacggctcat caggaggcag tgcatcagat gccatttccg gtgatgccag caatacccag
     2041 accaccaccg tggatatgaa gtctgcgctg tatcaggatg tgaaaaatgc ggtcacatca
     2101 atgctgaccc cgggtatcgg acgcatgttc atgtccacgg gctttatcac cgtcacagat
     2161 aatccacagg ttctggaaac ggtccgggca ttcattgaaa aacgcaatga agagatgaaa
     2221 cgtcaggtgg tgctgaatgt ggaaattctg agtatccgga aaacccgcaa tgagcaggcc
     2281 ggtatcgact ggaacgcagt cttcagcgac agaacgctgg gactgagtct cggcagcaca
     2341 ttcaccagtg cagccagtga tgccatcaca ggtggtgttt ccattgtgga cggtaaactc
     2401 accggttcaa aagccttcct gaaagcattg tcttcccagg gggatgtcag tgtggtgacc
     2461 cagaactctg ccgtcaccaa aaacctgacg ccggtgccaa tgcagatcgc caatcagcaa
     2521 agttacatcg agagtgtgac gacggatacc acagccaatg tcggctcatc cacctccctg
     2581 aatgcggcca ccatcaccac cggttttaac atgacccttc tgccgttcat tctcccggac
     2641 tcacagacac tgcagttgct ctattccatg agtctctccg acaaaccggt tattgagaat
     2701 tatgagagcg gtggaagtaa agcccagctg ccgaatgtgg atctgaaaac catcaatcag
     2761 accgtggatc tgaaatccgg gcagacggtc attatcagtg gcttccagca gtcaggacgt
     2821 cgcagcggaa aacagggtgt aggcacgccg ggattcttcg gtcttggcgg tgggatcaac
     2881 agtgaaaacg acgacaccat tctcgtggtg ctgatcacgc ccaatatcat ctgatttgca
     2941 caaggaggac gatcgtgacg gatatttcac cgacagggcg gcaggtgttt ccgctgacgg
     3001 cagggcgcaa tgtgtttctg gccggtctgg aatggaaaac actgccctca cagtaccggc
     3061 atgcccgcga ctttgcccgg gcacagaaag ccgacctttt cctggcctgt cagtatctca
     3121 gtaatgaaga tgccgatacc cacaccatgg tagccactgt cagtcgccgc atactgcccg
     3181 gaaaaccccg tcagtgcttc tccctcgccc tgctgatcct gccactactt gaacatggcg
     3241 gctatgccat cacggaactg acgttacccg gagaaacacc gcactatagc tttgtttccg
     3301 ttgtcgacgg tgttctcgtc agtgacctgg tgggaagcgg ggaagaggtt cgggaagccc
     3361 gggatacctt tctttccatc aacaccgaac caactcaggg ctggatacac tatgcaccgg
     3421 cggcattttc agcaggcgat cagaatcagc acctgccgct ggcctccctg actggctctg
     3481 gtaaacatcc ggctgccgca cgcctgaatc cggtatcccg gggaccgcag ttcatcactg
     3541 tctctctgat catcgccctt ctcggcactg catggtacgg ctggcagtat tatgaacgct
     3601 ggcagacaga aaaagccgcg caggcagcgg ttgcccgaca ggccacagag gaacgaatca
     3661 ctcccccctg gcccggactc cctgaaacag acgcgttcat ccggggatgt gctgatatat
     3721 ggacttcact gccactgtct gctgccggat ggcgttattc gctggcagaa tgctcgactg
     3781 acggtggcag cggcaatctt cgcgcatcgt acaccaatac tgcgggcgcg acagtgactg
     3841 acttcatccg gcgcatcagt gatatgacag acagccgacc gtttattgtg atgccggaag
     3901 gaaagaccgg cggcattggc ctgccggtca ccttccggct gtctgaaaac gccgtcacag
     3961 ttgatgcact accggaaacc gctgtcctgc aggaacgact gacctcgctg gctcagtcaa
     4021 tgcaactgaa actggactgg caggaagtta ataacagcct cactgatgag aacggacaga
     4081 tcattcagcc cccatggaaa gagtatgacc ttcagatgca gaccacactc cctgcacacc
     4141 atctggctga acgcctgaaa gagccttctg tccgttttat ctctgtcaca cggcaactgg
     4201 agaacggtcg tttccactat caatttacag gtaaatatta tgcccgataa agccaaaacc
     4261 cttctgtttt cagtcctgct gttaccggcc acggcaatgg ccacaaccgg aaatgcagag
     4321 caacagaaca cacagcaaat aaggattgaa cagatacagg aagagacagc cctgtatgaa
     4381 gccatgctgg cgagagagaa gatccgccat gaactgcgaa cacttgagca gggcgttccc
     4441 gcagaggccg gacgtcagca ggaaagcatt tccgcagtca caacaaatac acaggcatca
     4501 ctatcccccc gcctgacagc aatattcggt gttcagcctc agctgactgc ccggatagaa
     4561 cttccggggg gaagcatctc cgatgtgaag gcgggccaga acatccccgg aaccacttat
     4621 cacgtggaaa aaatcacgcc ggacagtgtg gtgctggaac agtctggcac cagaaaaacc
     4681 ctgcgaccat gagaccggcc gccgtaactg gcggctgtca aaaataaaag gatgaaagac
     4741 atattttccg ggcaccgctg cccgtaagga ggctttatgc tggatccagc agacctgccg
     4801 ccggatgaca ttctggatta tgtggccatc gacaccgata acgccggaca actgcggatc
     4861 agggttgttg agggaaaaaa acatctgcgt gccgtccagg aatatctgac ccgcctgcgc
     4921 gcacgctatc agggacgagt cggagacttt gaattcacca cactggatgt gatagcccgg
     4981 atccgggaag caacaacaat cgccggacag gaaaacggca tgaatcctgt gcagaataaa
     5041 atgctcgggt atatccgaca tgccgccacc cttaatgcct ccgatcttca tatcactccg
     5101 ggccgggaca acacagactt cacctatgtg gaggcccgtg tacatgggga actggaaatc
     5161 ctcgacatta tccggaaaga tgagggagtg gagcttctgg gggccaccta ctccgggatg
     5221 actgacgtca tcaagggaac acaatttgat cctggtgttc cccaggacgc ccgactggcg
     5281 gaaaagtttc tgaaacttgc ggggttgttt ggcgcacgtt actcccatta tccctgcgtg
     5341 gggggccttt atgctgttct gaggctgatc aaggacgaca gcagccatat tccgaccttc
     5401 ggtatgctgg gctatcatcc aaagcaggag cagaccatcc gccgtattct gcaacgaccg
     5461 gaaggtatca tcaccctgtc agggccgacc ggctccggaa aatccaccac gctgaggacg
     5521 gcgtctgcgg cctatcttga acagcacgga ttcaacaaca ccggtggtat tcttttgccc
     5581 cgtcgtcgtc tcttcaccat tgaatcaccg ccggaaggac gtattcccgg cgcaatccag
     5641 accgccgtca tggacagtgc tcagggctgg gtggattcca taaaatcagc cctgcggctt
     5701 gatcctgacg gcattctgaa cggtgaaatc cgcgatcacg attccgccat tacggcaata
     5761 aaagccgcca tgacaggcca tctgatgctg accaccatcc atgcgaacga cccgattaac
     5821 atcctcgaac gccttgagat ggaaggtgtt caggccagga tgattgcaga cccgcagttg
     5881 ttcatcggac tgctcagtca gcgtctggtt cagctgatat gtccccactg ccgtcatcca
     5941 tggaatgacg tggcatcccg tcgtactgag gatgaacgac gtctggtgga aaccttctgt
     6001 aatgctgatg cggtatatct gagaaaccat gacggctgcc cacactgctg gcgtggtgtc
     6061 acagggcgca cagtgattgc agaagtcatc tctcctgatg ccagattttt tcagttgtac
     6121 cgggaaaaag gccgcatcga agccaaaacc tactggcaca gagagctggg cgggatgacg
     6181 cgtaatcagc atctgctggg aaaaatcaac agcggactgg ttgaccctct ggcggcccat
     6241 tacatctctc ccattgatga ggacagctat acgctgctgc gctgatatga aatttctgga
     6301 aaaactcaac agtactgaac tggatctgtc tgccctgaac agctggacag ccagaaaaac
     6361 cttcggtctt aagcgtgccg agttctatga gtccctcggc ggaatgatac gtgacggaac
     6421 gccgtcactg cgcgccctgg agtttatctg taatgtcgaa actgactttg gcacccggaa
     6481 aggaacatcc ggcatgtact ttctggccag agaatgtatt gcctccctga aagccagcgg
     6541 tatgctgtcg ggggccctgc ccacatgggt tcccccggaa gaagcggcac tgatcaggaa
     6601 cggtgaagaa cgcggaaaca tcgccgaagc cctgttccag gtcgcaaaaa ccgtgaaagc
     6661 acggcgggaa atggtgagca gtctcatcac actctgtctt tatccccttc tgctgctttc
     6721 actctgtgtg gtgaatatgt acaacgcaca tacccgcatc attcccattg tcagcgcttt
     6781 tgcaccggag gagaaatggt cattccagat gaaagtcctt gcagccatgt cagagctggt
     6841 catcggctac ggtctgtggc tcagtggtgc catcatcacg cttgccatca tcatccgctt
     6901 ctcactcagt cgctataccg gcccgggacg cagacttctt gacagactgc caccatggtc
     6961 gctctatcag acttttcatg gtgtgaatta tctgttcagc atatcgtcaa tgctgcagat
     7021 aaatattccg ctttcacagg cgctgctcag aattgaaaaa agtgcggagc ataataaatg
     7081 gttaaaggag agaattcagg caataagaaa aaatgtatta tccggtcaga atctggccac
     7141 cgccatgcgc aattctgact atgattttcc ttcaaaaata tgcatcaata aacttctctt
     7201 aaattctgaa agggaagaca gcgtatctgc aatgaatacc tatgccggac agtggctgga
     7261 agatgcaaaa aagagcgtca gaaaaagtgg attaattatc accggtattt caggaggttt
     7321 tgtattcttt tttattctta acatgattag cgcgatatac agtatcagcg atatgctcaa
     7381 gcaataatac ctgccgtaac actaaaacgc ccttcaatat acttccatta acgtaacaac
     7441 cattaataca ggagtcatat atgaacttca gagcacgtct gtcttcttat ttttctttaa
     7501 aaaaagataa agaagaagat aaaggtttaa cattattaga aattgccggc gcaatgatta
     7561 tcatcgcaat agttgtcgcg ctcgcgatga gccagatgtc cagcacaatg gcctccagtg
     7621 ataaccagcg cgaactgaaa aacctgcaga caattgcgac caaaatgaag caatacaagt
     7681 tccagggaag atataccgga acggattata tcaaaaccct gacggaaagc ggtgcattgc
     7741 ctgctgatat gattgcagga ggcaacaaag ccaaaaacgc atggggtggt gatgtcacga
     7801 ttgctgccac agcagacaaa tatggctaca ttatcaccag taataatgtt cctaaaacaa
     7861 actgtgttga gctgattaac agcctgcgca gttcgtccat atttaccaaa attatgaaca
     7921 cagccccgaa catggtggat cccgttaccg tctgcagtag cgataaaaac aatatcaagc
     7981 tggaaaccaa cagctaatgc cctgccacac agcactccct ccggggtgct gtttcccgga
     8041 gcacataatc atgaaatggt tttatctctc atctcttttc ttattctgtc tcccggcgac
     8101 tgccgccgta aacagtaaca cactggatag ctgctttatt caggcaggaa aacgttactc
     8161 catcgatccc ggactactga aagtaattgc ccgtaaagaa agcagcctga atccccgggc
     8221 catcaatcat aacagaaatg ccagcggaaa aattatcagc acagattatg gcctgatgca
     8281 gattaattcc acacatattc ccggactgaa aagaatggga attatccgaa gtgaaaatga
     8341 actgctcagt aatgtttgcc tgaatataca gacaggtgca tggatccttg cccggcattt
     8401 tcagcagtgt ggtgtgaact gggagtgtct cggtagctac aacgccggat ttaataaaaa
     8461 taacactcac cgcagaatga aatatgcccg gctgatttat gccgcatata tgcagggacg
     8521 ttaataacgg agaatacgag ccatgttcct catcatctta ctcgtgatct ccgctacgct
     8581 gaccatacag ttacttgcca tcagactggc cggacattat ctcatgcagg tgcataccct
     8641 ccggcgtcca cgtcacctct ccctgctctg tgctgttctg gcactcaccg ccaccattac
     8701 gcacatcata cgggggacag acattgccgg actttgcttt ctgttgctga tggccggctg
     8761 catgacactg atgacactga ctgatgcagc ctcctgccgt cttccccgtc ccttcaccct
     8821 cacattcctg atgctgggtg tggcgtttcg tttatggcag ggcgatgctg ctattgcgct
     8881 tgcctgtgct ctgttctggt ttctcctgtt atctctcctg aggcgctaca cctcataccg
     8941 caagggagtg gagacgctgg gactggggga cgtgtttctg attgcaggtg tggccatgtg
     9001 gtcatctgcc aacagcactc cgtggatcat cggtattgcc gccgccagca caatgatctg
     9061 tatgtcgctg ttcccgcgca gtaatactgg ggccgctatt ccgttcggtc cgtttatctg
     9121 tgcatcactc tacattttca ctttttttcc tggtgccatt tttcacaccg gagggtaaca
     9181 ccatgcaaaa agacgacgat aaaggttttg ttctgtttga aattctggcc ggactgattg
     9241 tgatcggtat cgccacacca atgatttatt cagaaattga aaactggctg aatgagcagc
     9301 tttaccagag tgctgcatat catgcagatg cctacaatac cgcagccaga aactatattg
     9361 ccgacaacaa tgtccgtctg catagcggca gccttccggc aaatttcacg gctgatgatc
     9421 tgattcgtca gggatatctg aaacaggggt ttaaccattc tccctttggt cagagttata
     9481 tcaccggtat ccgccgtaac cagaccaccg ggcgtctgga agccctgacc tgctcaactg
     9541 gagggcagac aatcaaagaa gaagggctgc gtagtgtggc gggtcagttg ccggggctgg
     9601 gtggatttat cagcaaaaat ggaacggcaa caggcgcatt cggagcctgg acagataaac
     9661 cgggagacta cggacttacc tgttcgacag gtcacatcgc cgtcgtcatg agcggtgatg
     9721 acctgcagga aagtgaccgt ctctaccgtt ttcaggtagc aggtcgtcct gaactgaatc
     9781 agatgcatac cgcaatcaat atgggcggga ataacataaa caacaccggc aatatcaatg
     9841 gccagagtgc cacactgaaa ggtgatatta cgagtgaaga cggctggcta atcaccagaa
     9901 acaataaggg atggatgaat atcacacatg gtggtggatt caccatgaca gacagccagt
     9961 ggatacgcgc ggttaatggc aaaggcatca caaccaccgg ggaaatcaaa ggcggaaaag
    10021 tcagtggtgg tacagtccgg tctgacggtc gtctttcaac cggggagtat ttacagcttg
    10081 ataaaacagc tacggcaggc acaaagtgtg caccggacgg acttgtaggc cgcaccagta
    10141 caggggccat actttcgtgc caatccggta tgtgggcagg ttttgaatcc taccctgtcg
    10201 gaagccctat accgtggcca tcagccaccc ctccacaagg ctacctggtg atgaacgggc
    10261 agtcattttc atgttcccgg tatcctcagc ttgcccgtgc ttatcccggg tgtaagctgc
    10321 cggatttgcg cggtgttttt atccgtggct gggacaacgg acgtgggctg gatggagatc
    10381 ggaacagaca tctcctgagt tatcaggccg atcaatccgg tgtctatcat gagcgtgggg
    10441 gatggctgaa agggcatcac agcggtatgc cttactgggc ccagggaagc acaacggaaa
    10501 tgcgccccaa aaacattgcc tttaactaca tcgtcaaagc aagctgatgc atttaaggcg
    10561 tattaacttt taacttacag caacgtattt tgacccagat catgcttccc gaatatcccg
    10621 gtttacctct gtacgctctg aatcctgccg caccatagcc tgctttgccg tcacagacgg
    10681 caaagccgtt tgagccacta ccccagttcg cataaaccgg ttttctggag tcttcttctg
    10741 cgtcataaca ggccgaataa tcaaaaacca gtgcatcgct gatattattc ttcctgacac
    10801 cggattgttg ctgcgtgcac tggcccaggc tgctcagcgg ggtgaacgtg tccccacacc
    10861 tggcgagcca cccgcaaaca ttgatgaacg ggtcatgatt tgtccgttaa caaacagcca
    10921 gttcatgaca aaagcgaaca gtattttcat atcctcctcc tgaacaaata atttttgttc
    10981 aaaattaacg ctatgagacc ctttatgaaa cacttatcct cccgtgcagc cactgcggat
    11041 aataatcgta tcgttacaat tgaagacgtt tacacaatcg cattctcgca accaattcac
    11101 accgaatcag cgttggggca aaggtggatg ttgatccata ctggtgaata tgaaagtgcc
    11161 agcgatgcaa aacgcttcct tgatacgctc gtcaccgggc actgtgcttg catggtaaca
    11221 ccatcacctg ttctggatca tgacacctgg ctgcgtatcc gcctgctgat gggcgagata
    11281 agggatgcca aataaacatt catggcccca acacataaaa ataacgccgg ggactctggc
    11341 agaggtacca ttctttttac atagag
//
LOCUS       DAZFXT010000140        11316 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.140, whole genome shotgun sequence.
ACCESSION   DAZFXT010000140 DAZFXT010000000
VERSION     DAZFXT010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11316
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.140"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..132
                     /locus_tag="ACHKW0_004071"
     CDS             <1..132
                     /locus_tag="ACHKW0_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001417766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HFO7476815.1"
                     /translation="IYYLGYDRFLLIFFTDYSHFMDRITLPSTDTARGKGKWKIITA"
     gene            111..215
                     /gene="speFL"
                     /locus_tag="ACHKW0_004072"
     CDS             111..215
                     /gene="speFL"
                     /locus_tag="ACHKW0_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leader peptide SpeFL"
                     /protein_id="HFO7476816.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            526..2724
                     /gene="speF"
                     /locus_tag="ACHKW0_004073"
     CDS             526..2724
                     /gene="speF"
                     /locus_tag="ACHKW0_004073"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004586 - ornithine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HFO7476817.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            2721..4040
                     /gene="potE"
                     /locus_tag="ACHKW0_004074"
     CDS             2721..4040
                     /gene="potE"
                     /locus_tag="ACHKW0_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015496 - putrescine:ornithine antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:0015847 - putrescine transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HFO7476818.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(4096..5736)
                     /gene="pgm"
                     /locus_tag="ACHKW0_004075"
     CDS             complement(4096..5736)
                     /gene="pgm"
                     /locus_tag="ACHKW0_004075"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004614 - phosphoglucomutase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase
                     (alpha-D-glucose-1,6-bisphosphate-dependent)"
                     /protein_id="HFO7476819.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNALGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(5762..6307)
                     /gene="seqA"
                     /locus_tag="ACHKW0_004076"
     CDS             complement(5762..6307)
                     /gene="seqA"
                     /locus_tag="ACHKW0_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HFO7476820.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSASSQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVRGTI"
     gene            6492..7256
                     /gene="ybfF"
                     /locus_tag="ACHKW0_004077"
     CDS             6492..7256
                     /gene="ybfF"
                     /locus_tag="ACHKW0_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7476821.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            7396..7689
                     /gene="ybfE"
                     /locus_tag="ACHKW0_004078"
     CDS             7396..7689
                     /gene="ybfE"
                     /locus_tag="ACHKW0_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HFO7476822.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            7829..8359
                     /gene="fldA"
                     /locus_tag="ACHKW0_004079"
     CDS             7829..8359
                     /gene="fldA"
                     /locus_tag="ACHKW0_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HFO7476823.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            8569..8655
                     /gene="uof"
                     /locus_tag="ACHKW0_004080"
     CDS             8569..8655
                     /gene="uof"
                     /locus_tag="ACHKW0_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HFO7476824.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            8648..9094
                     /gene="fur"
                     /locus_tag="ACHKW0_004081"
     CDS             8648..9094
                     /gene="fur"
                     /locus_tag="ACHKW0_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HFO7476825.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(9178..9504)
                     /gene="chiQ"
                     /locus_tag="ACHKW0_004082"
     CDS             complement(9178..9504)
                     /gene="chiQ"
                     /locus_tag="ACHKW0_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChiQ/YbfN family lipoprotein"
                     /protein_id="HFO7476826.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(9554..10960)
                     /gene="chiP"
                     /locus_tag="ACHKW0_004083"
     CDS             complement(9554..10960)
                     /gene="chiP"
                     /locus_tag="ACHKW0_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HFO7476827.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         2751 a         2770 c         2824 g         2971 t
ORIGIN      
        1 atttattatt tggggtatga tcgttttttg ttgatcttct tcacagatta tagccatttc
       61 atggatagaa taactctacc ttcaactgac acagcaagag gtaaaggtaa atggaaaata
      121 ataaccgctt aatgcctcat ataaggcgga caacccatat catgaagttt gcccatcgta
      181 atagttttga ctttcatttc tttaatgccc gctagtcttc tgactaaagg gcaccccaac
      241 gtacaggtct ccctgacttt aagcattaca ggttaatacc tgtattcctc ggtgctcata
      301 tactgctaac ccttttaaac tctaaataat tcgagtcgca gcacttgcaa cttgaggtat
      361 gacgagtata gccagttacc gggctggtct gggttattgc atctgcaaaa agcaaactac
      421 tgatttattt atcagcggtg gagctttgct ttttttccgg cgtgatcgat ttctcctttg
      481 agaaattgag gacctgctat tacctgaaat aaagagatga acaaaatgtc agaattaaaa
      541 attgccgtta gtcgttcttg cccggattgt ttttccactc atcgtgcatg cgtgaatata
      601 gacgaaagta attatattga cgttgccgcc attattttat cagtcagtga tgttgaacgt
      661 ggaaaactcg atgaaataga cgctactggc tatgacattc ctgtttttat tgcaacggaa
      721 aatgaagaac gtgtacccgc agaatatctg ccacgtattt ctggtgtctt cgaacattgc
      781 gaatcacgta aagagtttta tggacgtcag ttagaaaccg ctgccagcca ttatgaaacg
      841 caactgcgcc cgcccttctt ccgcgcactg gtcgattatg ttaatcaggg caatagtgct
      901 tttgactgcc ctggtcatca ggggggtgag tttttccgtc gccatccggc aggcaatcag
      961 ttcgtggaat actttggcga aatgctcttc cgctctgact tgtgtaatgc tgacgtagca
     1021 atgggcgatc tgctgattca tgaaggtgcg ccatgtattg cacaacagca tgcggctaaa
     1081 gtctttaatg ccgataaaac ctatttcgtt ttaaacggta cttcatcttc taataaagtg
     1141 gtcttaaacg cattgctgac gccgggcgat ctggtgttgt ttgaccgcaa taaccataaa
     1201 tctaaccacc acggtgcgtt gctacaggct ggcgcgacac cggtttatct ggaaaccgca
     1261 cgtaacccgt atggctttat cggtggcatt gatgcgcact gttttgaaga aagttacctg
     1321 cgtgagctga tcgcggaagt cgcaccgcag cgggcaaaag aggctcgtcc tttccgcctc
     1381 gctgtgattc agttaggcac ctacgacggt acgatttata acgcccgcca ggtggtggat
     1441 aaaattggtc atctgtgtga ctacattctg tttgactccg catgggttgg ctatgagcaa
     1501 tttatcccga tgatggctga ctgttcgccg ctgttgctgg atcttaatga gaacgatccg
     1561 ggtattctgg ttacgcaatc cgtacataaa cagcaggctg gtttttctca aacttcacaa
     1621 atccataaaa aagacagcca catcaaaggg caacagcgtt atgtaccgca caaacgcatg
     1681 aacaacgcct ttatgatgca tgcctccacc agcccgttct atccgctgtt tgccgcactg
     1741 gatatcaacg ccaaaatgca tgaaggtgtc agcggtcgta atatgtggat ggattgtgtg
     1801 gtaaatggca ttaatgcccg caagctgatc ctcgataact gtcagcatat tcgtccgttc
     1861 gtacctgaac tggtggatgg taaaccctgg cagtcgtatg aaacagcgca aattgcggtt
     1921 gatctgcgct tcttccagtt tgtaccaggt gagcactggc actcttttga aggctatgca
     1981 gagaatcaat actttgtcga tccatgcaaa ctgttgctga caaccccagg tattgatgca
     2041 cgtaacggcg aatatgaagc gttcggtgta cccgcgacga ttcttgctaa cttcctgcgc
     2101 gaaaatggcg tagtgccgga aaaatgcgat cttaactcca tcctcttcct gctgactccg
     2161 gcagaagata tggccaagct tcagcaactt gttgccctgc tggtacgctt cgaaaaactg
     2221 cttgagtccg acgcgccatt agcagaagtg ctgccttcca tctacaaaca gcatgaagag
     2281 cgctacgccg gttataccct gcgtcagttg tgtcaagaaa tgcatgattt gtatgcccgt
     2341 cacaacgtga aacaactgca aaaagagatg ttccgtaagg aacacttccc acgcgtaagc
     2401 atgaatccgc aagaagccaa ctacgcctat ttacgcggtg aagtggagct ggttcgtctg
     2461 ccggatgcag aaggccgtat cgctgccgaa ggtgcgcttc cttatcctcc gggtgtgctg
     2521 tgtgttgttc cgggtgaaat ctggggtggt gctgttttgc gttacttcag cgctctggaa
     2581 gaagggatca acctgctgcc aggttttgca ccggagctgc agggtgtcta tatcgaagaa
     2641 catgatggtc gtaagcaagt ttggtgctat gtcatcaagc ctcgtgatgc gcaaagcacc
     2701 ctgttgaaag gggaaaaatt atgagtcagg ctaaatcgaa caaaatgggc gtcgttcagt
     2761 taaccatact gacgatggtc aacatgatgg gctccggtat catcatgctg ccgacaaagc
     2821 ttgccgaagt tgggacaatt tcaattatct cctggctggt gacagccgta ggctcaatgg
     2881 cactggcatg ggcattcgca aaatgcggta tgttcagccg taaatcaggc ggtatgggcg
     2941 gttatgcgga gtatgctttc ggtaaatccg gtaactttat ggcgaactat acctacggcg
     3001 tctcgctgct gattgctaac gtcgcgattg ctatttcggc ggttggttac ggcaccgaac
     3061 tgctcggcgc gagtttgtcg ccagtgcaga ttggtcttgc gaccatcggg gtgctgtgga
     3121 tttgtaccgt ggctaacttt ggtggtgcgc gcattaccgg gcaaatcagt agcattaccg
     3181 tgtggggggt cattattccg gtggtaggcc tgtgcattat cggctggttc tggtttagcc
     3241 cgacgcttta cgttgattcc tggaatccgc atcatgcacc gttcttcagt gcagtaggtt
     3301 cttccatcgc tatgacgctg tgggcttttc tcggtctgga gtctgcgtgt gcgaatactg
     3361 atgtagtgga aaacccggaa cgtaatgtgc caatcgcggt actcggcggt acgttaggtg
     3421 cggcggtgat ttatatcgtc tccaccaacg tgattgcagg gattgtgcct aatatggagc
     3481 tggcaaattc aacggcacca tttggcctgg ccttcgcaca gatgttcaca ccagaagtgg
     3541 gtaaagtcat tatggcgctg atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt
     3601 tcaccattgc ccaggtgttt aaatcttcat ctgatgaagg ctacttccct aaaattttct
     3661 cccgtgtaac caaagtggat gcaccggtgc agggaatgtt gaccattgtg attatccaga
     3721 gcgggttggc gctgatgacg attagcccgt cgctaaacag tcagttcaac gtgctggtta
     3781 acctggccgt ggtgaccaat atcattccgt atattctgtc aatggcggca ttagtcatta
     3841 ttcagaaggt cgctaatgtg cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg
     3901 ttggcgcgat gtatagcttt tatgcgctgt actcatccgg ggaagaagcc atgctgtacg
     3961 gttccatcgt gaccttcctt ggttggacac tgtatggtct ggtatcacca cgctttgaac
     4021 tgaaaaataa acacggttaa aaaagcaaag taaaaaaggg cgatcttgcg accgcccttt
     4081 ttttattaaa tgtgtttacg cgtttttcag aacttcgcta acaatctcaa ccgcttcttt
     4141 ctcaatctgc ttgcgatgtt cttcaccgag gaagctttcg cagtagatct tatatgcgtc
     4201 ttccgtgcct gacggacgcg cggcgaacca gccgttgtca gtcatcactt tcagaccgcc
     4261 gatagaagca ccattacccg gtgccgccgt cagacgtgcg gtgatcgggt cacctgccag
     4321 ggtgctggcg ctcaccattt ccggagacag cttagacaac gccgcttttt gtgcggaagt
     4381 cgcagcggcc tgcaaacggt tgtagctcgg cgcaccaaag cgttttgcca gttcgttgta
     4441 gtgttcctgc gggttcttac cggtgacagc ggtgatttcc gccgccagca gacacataat
     4501 gatgccgtct ttatcggtgg accacggcgt gccgtcgaaa cgcaggaagg aagccccggc
     4561 gctctcttcg ccgccaaagc cgaaactgcc gtcgaacaga ccatcaacaa accatttgaa
     4621 tcctaccggg acttctacca gtttacggcc caaggcgttg accacacggt cgatcatcgc
     4681 agaagaaacc agcgttttac cgacagcaac atctttgccc cactgcggac gatgctggaa
     4741 caggtagttg atcgccaccg ccaggtagtg gttcggattc atcaaacctg ccggagtgac
     4801 gataccgtga cggtcataat ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag
     4861 tgccagcagg cccgccatcg cacactcgga ggagcagtcc atacggatcg cgccgtcttt
     4921 atcaaggtgc ataaagcgga aggtttgatc gacctgatcg ttaacgatgg tcaggttgag
     4981 gttgtaatac tcgccaatac gcttccagta ttcgataccg gaaccgccca gcggatcaac
     5041 gccaagcgtc aggcccgctt tctgaatcgc cgccatatca acgatatcgg ccagaccttc
     5101 cacaaacggc tgcaccagat cctgctcttt cacatgaccg gatgccatcg cttcgtcgag
     5161 agagatacgc ttcacgcctt tcaggccatc ggccatcagt gcgttggccc tgtcttccac
     5221 cactttggtg acgttggtat cagccgggcc accatttggc gggttgtact tgataccacc
     5281 atcttccggc gggttatggg acggtgtaat cacgataccg tctgccagcg ggccaccttt
     5341 tttattgtga accaggatgg cattggaaac ggcaggcgtc ggagtgaatc cattgttttc
     5401 ctgcacaatg acatcaacgc cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg
     5461 ctcggacagg gcgtgagtat ctttacccac atagcaaggg ccagtgatgc catttttcgc
     5521 acgttcttca gcaattgcct gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg
     5581 cgctgcactg ccacggtgac cggaagtacc gaatttcacc gcgtgctccg cattccctgc
     5641 ttctggtttc agtacataat attgcgccgt cagttgggcg acgttaatca aatcactctg
     5701 ttgtgcaggt tggcctgcac gattgtggat tgccattgct ttgtcctttg tctgcaacgt
     5761 tttagatagt tccgcgaacc ttctcaatca attccgccgg gaattgcatc gactgcatga
     5821 tgtgttcgat catgctgcat ttacggccgg tgttggtgtt ggtgatcacc caatacggcg
     5881 tgcctggcac atgtttcggc ttggtctgat taccattttt cagcagcgtt tgttcatctg
     5941 ccgcaaagta aacgcgtgta cgaccgtgca acgattccgt tgcttcggca aacgcctggg
     6001 cgtcaagaga atatagtgta gacaacagca gcataaagcg attgaccgct cgcttttgct
     6061 ctgcgtattc gtccgaaagc agaagttcac gcattgcgcg aaccttgtct ttaatcgttt
     6121 tgaccggctt cgcttcgacg atagcaggtg acgcaacgcg aacctctttc gtcaccggag
     6181 cagcaggctg tgatgaggcg gaaaatttca acatacgccg taaaatgtcg gatgcgctct
     6241 cgccgatatg cttagtgtgg ctggcaatat agctgtagag ttcatcatca acttcaatcg
     6301 ttttcatctt aatccagtgc agtgtcttag ctgaatacaa gtcgccagga gtatatgaat
     6361 aaatcccggc agcggatagc gtcaagcctg gcttgcagaa aaactcggaa taaaccttat
     6421 ttgttgcagc cctgtggcac aatggtcaac ataataccct aacccgacaa cgcgaaaaaa
     6481 agaactttgc catgaaattg aatatccgcg cgcaaactgc acaaaaccag cacaataatt
     6541 ctcccatcgt tcttgtccat ggtctgtttg gtagcctcga caaccttggc gtactggctc
     6601 gcgatctggt aaacgatcac aatatcatcc aggttgatat gcgtaaccac ggtctttcac
     6661 cgagagatcc ggtaatgaat tacccggcga tggcgcagga tcttgttgat actctggatg
     6721 cacagcagat cgacaaagca acatttatcg gtcactccat gggcggtaaa gcggtaatgg
     6781 cacttactgc actggccccc gatcgcatcg ataaactggt ggcgatcgat atcgcaccgg
     6841 tcgactatca cgtacgccgt catgatgaga ttttcgcggc tatcaacgcg gtcagtgaat
     6901 cggacgcaca aactcgccag caagcagcag caataatgcg ccagcatctt aatgaagaag
     6961 gggtgattca gtttctgttg aaatcttttg ttgacgggga gtggcgcttt aacgtgccgg
     7021 tattgtggga tcagtatccg catattgtgg gttgggagaa aatcccggca tgggatcacc
     7081 ctgccctgtt tatccctggc ggcaattctc cgtatgttag cgagcagtac cgtgatgatt
     7141 tactggcaca atttccacag gcacgggcgc atgtgattgc aggcgcgggt cactgggtcc
     7201 atgctgaaaa accggatgcg gtattacgcg ctatccgtcg ctatctcaat gattaacgca
     7261 actgattaaa aacccagcgt ccgcgtcgct ttccgcgcgt gggcgttggc gtgactcatc
     7321 gcgctgatgt attatggcgc gctatccatc cgggcagagg cctggctgat tgtttccccc
     7381 gaagtcacca agatcatggc caaagaacaa acggaccgta cgacattaga tctgttcgcg
     7441 cacgagcgtc gaccgggacg accgaaaact aatccgcttt cgcgcgatga acagctgcgt
     7501 attaataaac gcaaccagct aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg
     7561 aagctgaacg cggaagctgt cgaggcgctg aacgagctgg cggagtcgcg caatatgagc
     7621 cgtagcgaac tgatcgaaga gatgttaatg cagcaactgg cggcgctgcg tagtcagggc
     7681 atcgtttaaa tttccacttt catgtagcac agtgtgcagt cctgctcgtt tgctgattcc
     7741 gcaagactgc ctgttctgct atgattgcct ttatccgtgg gcaattttcc acccccattt
     7801 caataagttt caagaggtta tttcactcat ggctatcact ggcatctttt tcggcagcga
     7861 caccggtaat accgaaaata tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc
     7921 cgatgtccat gacattgcaa aaagcagcaa agaagatctg gaagcttatg acattctgct
     7981 gctgggcatc ccaacctggt attacggcga agcgcagtgt gactgggatg acttcttccc
     8041 gactctcgaa gagattgatt tcaacggcaa actggttgcg ctgtttggtt gtggtgacca
     8101 ggaagattac gccgaatatt tctgcgacgc attgggcacc atccgcgaca tcattgaacc
     8161 gcgcggtgca accatcgttg gtcactggcc aactgcgggc tatcatttcg aagcatcaaa
     8221 aggtctggca gatgacgacc actttgtcgg tctggctatc gacgaagacc gtcagccgga
     8281 actgaccgct gaacgtgtag aaaaatgggt taaacagatt tctgaagagt tgcatctcga
     8341 cgaaattctt aatgcctgat gtgatgcggc gtagactcat gtctacgccg tattaataga
     8401 taatgccaat caaaataatt gctacaaatt tgtaactttt gctgttgtac ctgtacaatg
     8461 tcccggtgtt caagtggcct tgccgttgta aatgtaagct gtgccacgtt tttattaaca
     8521 atatttgcca gggacttgtg gttttcattt aggcgtggca attctataat gatacgcatt
     8581 atctcaagag caaattctgt cacttcttct aatgaagtga accgcttagt aacaggacag
     8641 attccgcatg actgataaca ataccgccct aaagaaagct ggcctgaaag taacgcttcc
     8701 acgtttaaaa atcctggaag ttcttcagga gccggacaac catcacgtca gtgcggaaga
     8761 tttatacaaa cgtctgatcg atatgggtga agaaattggt ctggctacgg tatatcgcgt
     8821 actgaaccag tttgacgacg ctggtatcgt cacccgccac aattttgaag gcggtaaatc
     8881 cgtatttgaa ctgacacagc aacatcacca cgatcacctg atttgcctcg actgcggcaa
     8941 ggttatcgaa tttagtgatg attccatcga agcgcgtcag cgtgaaattg ccgcaaaaca
     9001 tggcattcgc ctgactaacc acagtctcta tctttacggt cactgtgccg aaggcgattg
     9061 ccgcgaagat gagcacgcgc acgaaggcaa ataagccagc ctgaacgaga aaagccaacc
     9121 tgcgggttgg cttttttata caagggaaat aagaaaagcg agagttacag ctctcactta
     9181 tttgttatta ctgcgtattt cctgccagac cttatcacaa tcggctttca ccgcctgatc
     9241 atttccggtt tgcgttgcac gcagacactg ctgataatcc agtacgcgta cactttcctg
     9301 gtcggcaaac tgctgatgtt gtttctcttt cttcagcacg tttaacacgc tctggcaggc
     9361 ttcaattttt tctggcgaac cttgtgcggt gttgatacag gcgctgtatg cctctttcag
     9421 acgactgtct tcctgtggcg cggttgattg cgcacaagct accagccccg atgccattat
     9481 ggcgatgaga atcagttttt tcatagcaaa ttcctcaaac gtgcggcagg cacaaaacct
     9541 gccgcgtcgg gcatcagaag atggtgaatg gtgcgattac cataaatttc acgtcacgct
     9601 catcctggaa gatgttaccg taaccgccgc cccagcttgg gatgtcagag tggttgtcgt
     9661 attcggtgaa gtgcagtttg aacatcgtgc ctttggcgcg accgtcctga atggtgtaga
     9721 ccgcatccag gctgtaggca gactcttcaa tagtccggtt tttgtcgtag tacgcatccg
     9781 gattgctctg ccaggtcgca ggtttagcat cccatgcgta aacgtaggaa gcgccgatgg
     9841 cgaagcctgg aagattccag tttttcaggt catacatcgc accgaagaag accgcttttt
     9901 cgccgttggc gttgaagtcg gaacggttat cccaccagat atccaggcgg ccgttggagg
     9961 aagcgtaggt tggagtcata cgttgcagga agtatccctg ctgaccgtca gccttaaccc
    10021 aggtgccttc gaggcgcaaa tccactacgt cagccgcccg gtaaccaaag gtcaacgctt
    10081 gcagccaggc ggtgccgtca taaaggtcgt tgacgctgcg atcgtcaact ttatcgcgcg
    10141 taccatagaa ctggtagctg gtggttaacg ggctaccggc gatatcaaat ttgtagctgg
    10201 ctttggcaaa atattgatcg atataccctt ccgcctgacc aaatgccgct tccagtacga
    10261 agttattttt gaagtcgtat ttcgcaccga tggagtgcag gtaatcaact ttggtggttt
    10321 tatcgttctg ataaaactca tccatttcca gatgccacgg cgctttgtat tcgttggtcc
    10381 acatgtagga gaaactcaag gcaccagcat cgccgtaatc aaaattcgcc ccggcttccg
    10441 ccccctgata agtacctggc ataaagctcc agtgcggcgc taacagcgtt tgaccagttg
    10501 gctgaatgta acctgccctc gcccaaaccg gaccgtattt aaatttggcc gcagctttat
    10561 acaggcttat accgcttttg tcgccggacc agtcttcgtc ataggcttta ttactttttg
    10621 aaaacgcgat ttcgttcggg tgggagctgt cgccgttttc cgccatttca atcgccgtaa
    10681 acgcagcaat atcaaggccg aacatatcag cagcataacc ggactgaaaa tcgaggttgg
    10741 cattccaggt ggagtgagaa aggttggttt tgtatttgtc gccgtcggta acatctttac
    10801 ggtcgcgttc acgctgccag taatatatac cgccggttaa ggttgaatcg tcgatgaatc
    10861 cttcggcgcg agcctccggc attgccataa agcccgacat tgctgtaaca ccggcgatag
    10921 ccagcgccag cgtactacgt ttgccactaa acgtacgcat gggttaatcc tctttgacgt
    10981 ataaattgct gcaccaaagg tgaacagcga aaaaataaaa attaaaaaag tagcgttgag
    11041 aaaagtctcg ctgactacag gaacagacta tttttcgcga cgcgaattat caatctcttt
    11101 ctgtaaccag aatgtaagaa catgagcaac cgcacatatt ttgatgattt ttgttacaag
    11161 gcttaagttt gcggttgaaa ggcttgcaga agaataaaag cgtgaatttc cagcaagatg
    11221 aaagcggtta cattttattt aattatggtt tgtgatttta aatttatttt atggataaat
    11281 tgcgaagcgg aattaattcg cgaagaaaag cagaaa
//
LOCUS       DAZFXT010000141        11311 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.141, whole genome shotgun sequence.
ACCESSION   DAZFXT010000141 DAZFXT010000000
VERSION     DAZFXT010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11311
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.141"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            294..875
                     /gene="mdaB"
                     /locus_tag="ACHKW0_004084"
     CDS             294..875
                     /gene="mdaB"
                     /locus_tag="ACHKW0_004084"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HFO7476828.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            906..1220
                     /gene="ygiN"
                     /locus_tag="ACHKW0_004085"
     CDS             906..1220
                     /gene="ygiN"
                     /locus_tag="ACHKW0_004085"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HFO7476829.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1268..3160)
                     /gene="parE"
                     /locus_tag="ACHKW0_004086"
     CDS             complement(1268..3160)
                     /gene="parE"
                     /locus_tag="ACHKW0_004086"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HFO7476830.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDRDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3189..3770)
                     /gene="yqiA"
                     /locus_tag="ACHKW0_004087"
     CDS             complement(3189..3770)
                     /gene="yqiA"
                     /locus_tag="ACHKW0_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HFO7476831.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3770..4597)
                     /gene="cpdA"
                     /locus_tag="ACHKW0_004088"
     CDS             complement(3770..4597)
                     /gene="cpdA"
                     /locus_tag="ACHKW0_004088"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004115 - 3',5'-cyclic-AMP
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HFO7476832.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4622..5044)
                     /gene="yqiB"
                     /locus_tag="ACHKW0_004089"
     CDS             complement(4622..5044)
                     /gene="yqiB"
                     /locus_tag="ACHKW0_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HFO7476833.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5045..5674)
                     /gene="nudF"
                     /locus_tag="ACHKW0_004090"
     CDS             complement(5045..5674)
                     /gene="nudF"
                     /locus_tag="ACHKW0_004090"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HFO7476834.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5879..7360
                     /gene="tolC"
                     /locus_tag="ACHKW0_004091"
     CDS             5879..7360
                     /gene="tolC"
                     /locus_tag="ACHKW0_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HFO7476835.1"
                     /translation="MKKLLPILIGLSLYGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7508..8179
                     /gene="ygiB"
                     /locus_tag="ACHKW0_004092"
     CDS             7508..8179
                     /gene="ygiB"
                     /locus_tag="ACHKW0_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HFO7476836.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8185..9345
                     /gene="ygiC"
                     /locus_tag="ACHKW0_004093"
     CDS             8185..9345
                     /gene="ygiC"
                     /locus_tag="ACHKW0_004093"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7476837.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQIEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9383..10171)
                     /gene="ygiD"
                     /locus_tag="ACHKW0_004094"
     CDS             complement(9383..10171)
                     /gene="ygiD"
                     /locus_tag="ACHKW0_004094"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HFO7476838.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10314..11087
                     /gene="zupT"
                     /locus_tag="ACHKW0_004095"
     CDS             10314..11087
                     /gene="zupT"
                     /locus_tag="ACHKW0_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HFO7476839.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11145..11309)
                     /gene="yqiD"
                     /locus_tag="ACHKW0_004096"
     CDS             complement(11145..11309)
                     /gene="yqiD"
                     /locus_tag="ACHKW0_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiD"
                     /protein_id="HFO7476840.1"
                     /translation="MAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYLNS
                     TREETAEKVE"
BASE COUNT         2802 a         3047 c         2807 g         2655 t
ORIGIN      
        1 tttataatta tcaacatgac acgttgatat tttcatcttt tacttattca aagccatatt
       61 ttcaatccca aaaaaaaccg aaccaaacgt gtaaattatt ccggtgcgag taagaattat
      121 gaggaatggc tatcagtatt gtcattttca gaaaatattt atcctgcatc ggtgagtcag
      181 agcaagatca gacttttgct aaattcgcaa aagactttgc acattttgct aatttcaccg
      241 taccgctctg tgacgtacta tagtcggcaa acgtctcacc ttgaggttaa aaaatgagca
      301 acatcctgat tatcaacggc gcgaaaaaat tcgcccactc caatggtcaa ctgaacgaca
      361 ccctgaccga agtcgcggat ggcacactgc gcgacctcgg gcatgatgtc cgcatcgttc
      421 gcgccgacag cgactacgat gtcaaagcgg aagtacaaaa ctttctctgg gctgatgtgg
      481 tgatctggca gatgccaggc tggtggatgg gcgcgccgtg gacagtgaaa aaatatattg
      541 atgatgtatt caccgaaggt catggaacgc tgtatgccag cgatggccgt acccgcaaag
      601 atccgtcaaa aaaatacggt tccggcggcc tggtacaggg caaaaaatat atgctttctc
      661 tgacctggaa cgcaccaatg gaagccttca ccgaaaaaga tcaattcttc cacggcgttg
      721 gcgtcgacgg tgtgtatctg ccgttccata aagcaaacca attccttggc atggaaccgc
      781 tgccgacatt tatcgctaat gacgtgataa aaatgcctga tgttccccgc tatactgaag
      841 aatatcgcaa gcatcttgtg gaaatttttg gttaactaga gctcaggctt tagaaggagt
      901 taaccatgct taccgtaatc gcagaaatcc gtactcgtcc tggtcaacat caccgtcagg
      961 cggtattgga tcagtttgct aaaatcgttc caaccgtact gaaagaagaa ggttgccacg
     1021 gctatgcgcc aatggtggat tgcgctgctg gcgtgagttt ccagtctatg gcaccggatt
     1081 ctatcgtgat gattgagcag tgggaaagca tcgcgcatct tgaagcgcat ctgcaaaccc
     1141 cgcacatgaa ggcgtacagc gaagccgtaa aaggcgacgt gctggagatg aatatccgca
     1201 tcctgcaacc tgggatttaa acctgccttg tttgcccggc catcctgacc gggcaatgtt
     1261 ctttccttta aacctcaatc tccgccatgt cgcctttctc ttgcaaccag ttgcggcgat
     1321 cttccgagcg tttcttcgcc agcagcatat ccatcatcgc gtcagtacgc tgatcgtctt
     1381 catcatcgat agtcaactgc accagacggc gagtgttcgg atcaagcgtg gtttcacgca
     1441 attgcatcgg gttcatttcc cccagacctt taaaacgctg gacgttcggc ttgcctttct
     1501 tgcgttttaa ttgctcaagt acgccctctt tctcttcttc cgtcagcgcg taataaacct
     1561 ctttcccgag atcaatacgg tagagcggtg gcagtgcgac gtaaacgtga ccgtgtttca
     1621 ccaacgcgcg gaagtgtttt acgaacaaag cgcagagcag cgtggcaatg tgcagaccat
     1681 cagagtccgc atccgcgagg atacagattt tgccataacg aagctggctc agatcgtcgc
     1741 ggtcaggatc gataccgatc gctaccgaaa tatcatgcac ttcctgcgag gccagcactt
     1801 cgtcggaaga aacttcccag gtgttaagga tcttaccttt cagtggcatg atcgcctgat
     1861 attcgcgatc gcgcgcctgc ttggcagatc cgcctgcgga gtcaccttcc acaaggaaca
     1921 gttcggtacg gttaagatcc tgcgcggtac agtcagccag tttgccaggc agtgctgggc
     1981 cgctggtcag ctttttgcgc accacttttt tagccgcacg catacggcgc tgggcgctgg
     2041 aaatcgccat ctccgccagc agctccgccg cctgaacgtt ctggttcagc cacaggataa
     2101 aggcatcttt caccacgcct gaaacgaatg ccgcgcattg acgcgaagag agacgctctt
     2161 tggtctgccc ggcaaactgc ggatcctgca ttttcaccga cagaacatag gcgcagcgat
     2221 cccagatatc ttccgccgac agcttcacgc cgcgcggcag aatgttgcgg tattcacaga
     2281 actcacgcat cgcgtccaac aggccctgac gcagaccatt aacatgggta ccgccctgca
     2341 tcgttgggat aaggttgacg tagctttcgg tcagcagttc accgccttcc ggcagccaca
     2401 gtagcgccca gtccacagct tcagtatcgc cagcgaaatt accgataaac ggtttttctg
     2461 gcagcgtcgg taaaccgttt accgcttccg ccaggtaatc attcagaccg tcctgatagc
     2521 accagcgctg ttcggtgttg ttgatctcat ctttaaaggt aatctcaacg ccggggcaca
     2581 gtactgcttt ggccttcagc acatgcgtca ggcgtgaaac agaaaaacgc gggctgtcaa
     2641 agaaggtttc atccggccag aagtgcacgc tggtaccggt attgcgttta ccgcaagtgc
     2701 cgacaacctg taaatcctgc accttttcgc cattttcaaa ggcgatgtta taaacctgac
     2761 catcgcggcg cacgttaact tctacgcgct tcgacagggc gttaaccacc gaaatcccca
     2821 cgccatgcag gccgccagag aactggtaat ttttgttaga gaatttaccg cctgcatgca
     2881 gacggcaaag aatcagttca accgccggta caccctcttc cgggtgaata tccaccggca
     2941 tcccgcgccc atcgtcaata acttctaacg actggtcagc atgtaagatt acatccacgc
     3001 gttttgcgtg acccgccagt gcttcatcga cactgttatc aataacttct tgccccaaat
     3061 ggttagggcg agtggtatcg gtatacatcc ccggacggcg gcgaaccggc tcaagcccgg
     3121 tgagtacctc aatggcatca gcgttataag tttgcgtcat ggtttaagtt agcaattcga
     3181 gttgatcgtc agagatggtg cagaccaaga aaatcgacga tcgggttgaa ataatcttcg
     3241 aagcccgtga atgcgtggtt gccgccttct atgacagtct ggcggcagga agcgtagtac
     3301 gccaccgcct ggcggtaatc cagcacttca tctcccgtct gttgcagcag ccagatcaaa
     3361 tccggcgctt ccagcgggtc aatctgcatg actttgagat cgtaaatatg gcgtgactct
     3421 agcacatatt gctgcccggt gtaggggttc tcgttctgac cgagatagtc cgtcagcagt
     3481 tcaaacgggc gcaccgccgg gtttaccacc actgcgggca gcataaaaca ttgtgacaac
     3541 caggtggcgt aatatccccc cagtgacgaa ccgacaatac ccagcgaatc gccgccatgt
     3601 tccaggacaa tggattccag cagctctgcc gcgtcggaag gatacggcgg caactgcgga
     3661 atgatcatct caacgtcagg gtgatgttcc gccagccagt tttttaacaa gctcgctttt
     3721 gcagagcgcg gcgagctgtt gaaaccgtgt aaataaagaa gcgtagacat cagtagcctt
     3781 ctgaagcggt atcaggttgg aaacgtgtgt ccgccaggcg atgcacctcg gtggtcagcg
     3841 tgccatcagc atgtaactcg agagtacgcc agccgggcgc gatagtatcc agcgtaaagt
     3901 tggaacagtg cggcttaaac tgcacacagg tcgacggcgt tgccagcagg cggcgaccgt
     3961 tccagtcgag atccagctcc tgatgaatat gaccgcacag caagtatttg acgtgcggaa
     4021 acttcgccag cacggtatcc agttcgcccg cgttacgcag actgtgttga tcgagccaac
     4081 tacaacccgc aggtagcgga tgatgatgca gcagcagcaa cgtatggcgt tctggcgcat
     4141 cggccagttt acgttccagc cactcaagct gaaactcgct cagctcaccg tgcggcacgc
     4201 caaacacctg gctatccagc aacaggattt gccattgctc accaataaac acgcgcttcg
     4261 ccggggagat acccgcatcc tgtaacgcgc tgtacatcgc gggctggaaa tcgtggttgc
     4321 ccggcagcca gacgcagggc gcacgaaaac ttgcgatgcc ttcagcgaaa tgctgatagg
     4381 ccgcagagga ttgatcctgc gctaaatcac ctgtcgcgac aatcaggtcg aattcgtgct
     4441 gatgtggacg aatcgcctcc agcaccgcct ggtagctttc ccaggtgttt acccctaaca
     4501 gggcttcgtg cttttgtgca aacaggtgag tatcggtaat ttgtaaaatc ctgactctgg
     4561 cctcaccagc cagaggaagg gttaacaggc tttccaaatg gtgtccttag gtttcacgac
     4621 gctagtaaac cggaatcgcc atcgctccat gtgctaaaca gtatcgcaac cagtccgcta
     4681 aaaactggtt aatttgatgc ttttcgtcgc gttgatgcaa ctttttatta ggataatcat
     4741 accgcgcttt gaagcgaaaa atctgctggc ttgaacacac ttcagccacc atcgcgtcat
     4801 gatacagacg caccgtcatt gacggaaggc tccagtaact gatcgcgggc gcagtctgtt
     4861 ctattgtcac cagggtagtg tatcgggtcg attccacaat cgtcagccga tattgtgcgt
     4921 ttgccacctg atagcttaca gtttcgccgg gtgcgtcatt gcgcggtaac aaacggcgca
     4981 attgtgaaaa gttcatctcg cacaggcgca tcatttcagg aaagtcaggt gtgtaacgct
     5041 tcatttatgc ccactcattt tttaacgctt gatgatgcag ctgcagccat tgcaaagcga
     5101 tgaccgacgc tgcgttgtcg attttcccct cttctaccca ctggtatgcc tgttcccggc
     5161 ttaccacatg aacgcgaata tcttcgtttt catcagccag accatgaata ccgcttgcgg
     5221 tcgtggcgtc cacttcgccc accataattg acgaacgctc actggtgccc cccgggcttg
     5281 ccaggaaact taacaccggt ttggtccgtt tgactatcag tcccgcctct tcaatcgctt
     5341 cgcgacgggc aacatcttcc acactttcac cctcttctat catcccggca accatctcca
     5401 gtagccaggg ggtttcactg gtgtcgtatg cggcaatccg aatctgctca atcagcacaa
     5461 cttcatcacg cactgggtca aagggtagca agactgcggc gtgaccgcgc tcaaaaattt
     5521 ccctccgtac ctcatgactc atttgcccgt tgaataaacg atgacgaaat ctataaagat
     5581 ctaatgaaaa aaagccgcga taaagtgttt ctcgtgcaat aatttctaca tcgtttttgc
     5641 caaatgtaac gggcaggttg tctggcttaa gcattgttaa tgtcctggca ctaatagtga
     5701 attaaatgtg aatttcagcg acgtttgact gccgtttgag cagtcatgtg ttaaattgag
     5761 gcacattaac gccctatggc acgtaacgcc aaccttttgc ggtagcggct tctgctagaa
     5821 tccgcaataa ttttacagtt tgatcgcgct aaatactgct tcaccacaag gaatgcaaat
     5881 gaagaaattg ctccccattc ttatcggcct gagcctttat gggttcagtt cgttgagcca
     5941 ggccgagaac ctgatgcaag tttatcagca agcacgcctt agtaacccgg aattgcgtaa
     6001 gtctgccgcc gatcgtgatg ctgcctttga aaaaattaat gaagcgcgca gtccattact
     6061 gccacagcta ggtttaggtg cagattacac ctatagcaac ggctaccgcg acgcgaacgg
     6121 catcaactct aacgcgacca gtgcgtccct gcagttaact caatccattt ttgatatgtc
     6181 gaaatggcgt gcgttaacgc tgcaggaaaa agctgcaggg attcaggacg tcacatatca
     6241 gaccgatcag caaaccttga tcctcaacac cgcgaccgct tatttcaacg tgttgaatgc
     6301 tattgacgtt ctttcctata cacaggcaca aaaagaagcg atctaccgtc aattagatca
     6361 aaccacccaa cgttttaacg tgggcctggt agcgatcacc gacgtgcaga acgcccgcgc
     6421 gcagtacgat accgtgctgg cgaacgaagt gaccgcacgt aataaccttg ataacgcggt
     6481 agagcagctg cgccagatca ccggtaacta ctatccagaa ctggcagcgc tgaatgtcga
     6541 aaactttaaa accgacaaac cacagccggt taacgcgctg ctgaaagaag ccgaaaaacg
     6601 caacctgtcg ctgttacagg cacgcttgag ccaggacctg gcgcgcgagc aaattcgcca
     6661 ggcgcaggat ggtcacttac cgacgctgga tttaacggct tctaccggga tttctgacac
     6721 ctcttatagc ggttcgaaaa ctcgtggtgc cgctggtacc cagtatgacg acagcaatat
     6781 gggccagaac aaagtgggcc tgagcttctc gctgccgatt tatcagggcg gaatggttaa
     6841 ctcgcaggtg aaacaggccc agtacaactt tgttggtgcc agcgagcaac tggaaagcgc
     6901 gcatcgtagc gtcgtgcaaa ccgtacgttc ctccttcaac aacattaatg catctatcag
     6961 tagcattaac gcctacaaac aagccgtagt ttccgctcaa agctcattag acgcgatgga
     7021 agcgggctac tcggtcggta cgcgtaccat tgttgatgtg ttggatgcaa ccaccacgct
     7081 gtacaacgct aagcaagagc tggcaaatgc gcgttataac tacctgatta atcagctgaa
     7141 tattaagtca gccctgggta cgttgaacga gcaggatctg ctggcactga acaatgcgct
     7201 gagcaaaccg gtttccacta atccggaaaa cgttgccccg caaacgccgg aacagaatgc
     7261 tattgctgat ggttatgcgc ctgatagccc ggcacccgtc gttcagcaaa catccgcacg
     7321 cactaccacc agtaacggtc ataacccttt ccgtaactga tgacgacgac ggggcttcgg
     7381 ccccgtctga acgtaaggca acgtaaagat acgggttatc tgccgcattc ttcccccttc
     7441 tcgcttcaat ttcgaccagc catcctctat tctgatgggt atttaccact ggtcccggaa
     7501 gacaaaaatg aaacggacaa aatccatacg ccacgcatcg ttccgcaaaa actggagcgc
     7561 gcgccatctg acaccagtcg ctctcgcggt tgccactgtt tttatgctgg ctggctgtga
     7621 aaagagtgat gaaacagtgt ctctctatca aaatgctgac gactgttcag ctgcaaaccc
     7681 aggcaaaagc gccgaatgta ccaccgcgta caacaatgcg ctgaaagaag ccgaacgtac
     7741 tgcgccgaaa tacgccaccc gtgaagactg tgttgctgaa tttggtgaag gtcagtgcca
     7801 gcaggcacca gcccaggctg gcatggcacc agaaaaccag gcgcaggccc agcaatccag
     7861 cgggagtttc tggatgccgc tgatggccgg ttacatgatg gggcgtctga tgggcggcgg
     7921 cgcgggattt gcacagcagc cgctgttctc ctcgaaaaac ccagccagcc cggcttacgg
     7981 taaatatacc gacgcgacgg gtaaaaacta tggcgcagcc cagccaggcc gcaccatgac
     8041 cgtaccgaag acagccatgg caccaaaacc ggcgaccacc actaccgtta cccgtggcgg
     8101 ttttggtgaa tctgttgcca aacaaagcac tatgcagcgt agcgcaactg gcacctcttc
     8161 tcgttcaatg ggtggctgat accgatggaa agagtcagta ttaccgagcg cccggactgg
     8221 cgtgagaaag cccacgaata cggtttcaat tttcacacca tgtacggcga gccgtactgg
     8281 tgtgaagatg cttactacaa gttgaccctc gcccaggttg aaaagctgga agaagtcacc
     8341 gccgaactgc accagatgtg cctgaaagtg gtggaaaaag tgatcgccag cgatgagctg
     8401 atgaccaaat tccgcattcc aaaacacacc tggagttttg tgcgccagtc atggctgacg
     8461 caccagccat cgctttattc gcgtcttgat ctggcgtggg atggcactgg cgaacctaaa
     8521 cttctggaaa ataacgccga tacgccaacg tcactatacg aggcggcgtt ctttcagtgg
     8581 atctggctgg aagatcagct taacgccggt aacttgccgg agggcagcga ccagtttaac
     8641 agtctgcaag aaaaactgat cgatcgcttc gttgagctgc gtgaacagta tggcttccag
     8701 ttgctgcatc tcacctgctg tcgcgacacg gtggaagatc gcggaaccat tcagtatttg
     8761 caggactgcg caacggaagc tgaaattgct actgagttcc tctacatcga tgatatcggg
     8821 ttaggtgaaa aaggtcagtt cacggattta caggatcagg taatttccaa cctgttcaag
     8881 ctgtatccgt gggaatttat gttgcgtgag atgttttcca ccaagctgga ggatgcaggc
     8941 gtacgctggc tggaaccggc gtggaagagc attatctcca acaaggcact tctaccgcta
     9001 ctgtgggaga tgttcccgaa tcacccgaac ctgctgcccg cttattttgc ggaagatgat
     9061 catccgcaaa tagaaaaata tgtggttaaa ccgatcttct cccgtgaagg cgcaaacgtg
     9121 tcgatcattg aaaacggcaa aaccattgaa gcagcggaag gtccgtatgg cgaagaaggg
     9181 atgattgttc agcaattcca cccgttaccg aaattcggcg acagctatat gctgattggt
     9241 agctggctgg tgaacgatca acccgccgga attggcattc gtgaagaccg tgcattgatc
     9301 acccaggata tgtctcggtt ttatccacat atttttgttg agtaatcaat gatgccggat
     9361 ggcattcgcc atccggtaat tgttaaccta tctgcaccga cagcatactc aggctgccca
     9421 tttctatacc atcgaccgga atggtaattg gctcctgccc atcccacgca cctaagacat
     9481 acaacaacgg cagatagtgc tccggcgttg ggttcgataa cgcgccacct tcatggtcga
     9541 ggtaattcac cagaggatgc tgctctactg ccccttgcca cgtcagattc gctttcacat
     9601 actcattaaa cgacatcgcc caaggatatg gtgaactatc accgtgccac ttcactgtac
     9661 gcaggttatg caccacgttg ccactggcga ccaacattat tccttcatct cgcagcgctg
     9721 ccagtttgcg ccccatttcg aaatgccagg cggcaggttt gctactgtcg atactcaact
     9781 gcaccatcgg gatatcggcg tcaggataca tcttaatcag cacgccccag gaaccgtggt
     9841 caaagcccca ggcttcttta tccagcgtca ccgggaccgg cgctaacagc tcaaccagat
     9901 gctgtgccag cacaggcgaa cccggagcag gataatgcgt atcgtacagc gcctgcggga
     9961 agccaccaaa gtcatgaatc gtgggcggcg tctccatcgc ggtcactcct gttccacggg
    10021 taaaccagtg agccgaaacc accacaatcg cttgcgggcg tggcaatgtc atccccaact
    10081 tctgccagct gcgggtatac aaattatctt ccagcacgtt catcggacta ccgtgaccta
    10141 aaaacaatgc tggcatacgt gttgaagaca tgatgatatc cttaactaaa ggtgtcattt
    10201 tgatatcctc acaatacgct tgttcggcgg agtaagaacc cggataacaa tgatgatgat
    10261 catcagttat tttgacgatc tgcctgaagg tgaagattta taaggagttg tcgatgtcag
    10321 tacctctcat tctgaccata ctggcggggg cagccacgtt tattggcgcg tttctcggcg
    10381 ttctcgggca aaaaccctcg aaccgcttac tggcgttttc gctaggattt gcggcgggga
    10441 tcatgttgct catctcatta atggaaatgc ttcctgccgc actagcagct gaaggaatgt
    10501 cgcctgtgtt gggttatgga atgtttatct tcggtctgct tggctatttt ggcctggacc
    10561 gcatgttgcc acatgcgcat ccgcaggatt taatgcaaaa atcggtgcag ccgttgccaa
    10621 aatcgatcaa gcgcacagcc attctgctca ctctcggcat cagtctgcat aacttcccgg
    10681 aagggattgc cacctttgtc acggcgagca gcaacctgga gctgggattt ggcatcgcac
    10741 tggccgtcgc tttgcacaat atccctgaag gtctggcagt ggcaggcccg gtttatgcgg
    10801 caacggggtc taaacgcacc gcgattctgt gggcggggat ttctggacta gcagaaattc
    10861 ttggtggtgt gctggcgtgg ttaatcctcg gtagcatgat ttccccggtg gtcatggcgg
    10921 caatcatggc ggcggttgca ggaattatgg tggcgctctc ggttgatgaa ttaatgccgc
    10981 tcgccaaaga gattgaccct aataataacc ccagctatgg cgtactatgt ggaatgtcag
    11041 tgatgggatt cagtttagtg ctgctacaaa cggcgggaat tggttaaaaa caacatcaga
    11101 agttgtccag cacatggaca acttctgcca gcttcacgaa agccttattc taccttttcg
    11161 gctgtctctt ctctcgtact gtttaagtat ttgttccgcg cttcaagcag tgcgtctctg
    11221 tcctggcgaa acgcccgaca ataacgtttc aaatgcagga aataacccac cacaaccaga
    11281 gcaatcaata caatccagta ccaggcgata a
//
LOCUS       DAZFXT010000142        11201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.142, whole genome shotgun sequence.
ACCESSION   DAZFXT010000142 DAZFXT010000000
VERSION     DAZFXT010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.142"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            205..1347
                     /gene="yghO"
                     /locus_tag="ACHKW0_004097"
     CDS             205..1347
                     /gene="yghO"
                     /locus_tag="ACHKW0_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YghO"
                     /protein_id="HFO7476841.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGVEPYKRYRLYEKQI"
     gene            complement(1354..2118)
                     /gene="glcC"
                     /locus_tag="ACHKW0_004098"
     CDS             complement(1354..2118)
                     /gene="glcC"
                     /locus_tag="ACHKW0_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HFO7476842.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTNLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2369..3868
                     /gene="glcD"
                     /locus_tag="ACHKW0_004099"
     CDS             2369..3868
                     /gene="glcD"
                     /locus_tag="ACHKW0_004099"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009339 - glycolate oxidase complex
                     [Evidence IEA];
                     GO_function: GO:0003973 - (S)-2-hydroxy-acid oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HFO7476843.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3868..4920
                     /gene="glcE"
                     /locus_tag="ACHKW0_004100"
     CDS             3868..4920
                     /gene="glcE"
                     /locus_tag="ACHKW0_004100"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HFO7476844.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEL
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4931..6154
                     /gene="glcF"
                     /locus_tag="ACHKW0_004101"
     CDS             4931..6154
                     /gene="glcF"
                     /locus_tag="ACHKW0_004101"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HFO7476845.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERILREGLRQVVPRPAVFRALTQVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAIRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6159..6563
                     /locus_tag="ACHKW0_004102"
     CDS             6159..6563
                     /locus_tag="ACHKW0_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlcG family protein"
                     /protein_id="HFO7476846.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6585..8756
                     /gene="glcB"
                     /locus_tag="ACHKW0_004103"
     CDS             6585..8756
                     /gene="glcB"
                     /locus_tag="ACHKW0_004103"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HFO7476847.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMDKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHAMHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            9111..10793
                     /gene="glcA"
                     /locus_tag="ACHKW0_004104"
     CDS             9111..10793
                     /gene="glcA"
                     /locus_tag="ACHKW0_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HFO7476848.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2629 a         2781 c         3103 g         2688 t
ORIGIN      
        1 tgaatatatt aagaagaagc cttgtccggt cgccttaaat cgacaaaagg cattgaggcg
       61 cttctgtgta gtctctaacc ttatcataat cattaataaa tactatttat tctttgtttg
      121 tattcattgg gttgattatt aaggtgtcgt tcgttgctgt tgcaacctgt ttttcaccgt
      181 taattatttg ttatccacag agatatggaa tgcgatctgc tgatgattaa aattgaaaaa
      241 gtaattaata aaaatgacct taaggcattt atcgcttttc cgtcatcact ttatcccgac
      301 gatccaaact ggataccccc tttattcata gaacgcaacg agcatttgtc tgcgaaaaat
      361 ccggggacgg accatatcat ctggcaggcg tgggtggcaa aaaaagcggg gcagatagtt
      421 gggcgtatta ccgcgcaaat agataccttg caccgtgagc gttacggtga agataccggt
      481 catttcggca tgattgacgc cattgatgat ccgcaggttt ttgccgcgtt gtttggcgca
      541 gcggaagcgt ggttgaagtc acaaggtgca agtaagatca gcggtccttt cagcctgaat
      601 atcaatcagg aaagcggatt actgattgaa ggttttgaca caccaccctg tgcgatgatg
      661 ccacacggca aaccgtggta tgccgcgcat attgaacaac tgggttatca caaaggcatt
      721 gatttactgg cgtggtggat gcagcgaacc gatctcactt tctctccggc gctaaaaaaa
      781 ctgatggatc aggtgcgcaa aaaggtgacc attcgctgca tcaatcgtca gcggtttgcc
      841 gaagaaatgc agatcctgcg tgagattttc aactctggct ggcagcacaa ctggggattt
      901 gtgccgttta ccgaacatga attcgcgacc atgggcgatc aacttaaata tctggtgccg
      961 gacgatatga tctatatcgc tgagattgat tctgcaccct gcgcgtttat tgtcggcttg
     1021 ccgaacatca acgaggcgat tgccgatctg aacggatcgc tctttccctt cggctgggca
     1081 aaattgctgt ggcgtttgaa agtcagcggt gtgcgaactg cgcgagtacc gctgatgggc
     1141 gtgcgcgacg agtatcagtt cagccgcatc ggcccggtga ttgcgctgtt attgattgaa
     1201 gccttacgcg atccgtttgc ccgccggaag atcgatgcgc tggagatgtc atggatcctc
     1261 gaaaccaata caggcatgaa taacatgctg gaacgcattg gggtggagcc gtacaagcgt
     1321 taccgattgt atgaaaagca gatttaatac cagctaactc aggttcatct ccagcggcac
     1381 cgccgagcga atcaaatggt ggccttccag ctcgatatcg tggagattct ttttcacggt
     1441 ccgcacatga tcgcgtgctg cgcgctgggc gacgtgcggc agccgctgca acaccgcgtt
     1501 gtagatcctc gcatgctggc gatcgatctg ctttttttgc tgtggtcgat ggtagagatt
     1561 atttacgctg gcaaacactg aattaaacat cagattggtc aatgattgca gcgtaaacac
     1621 cagcacctga ttgtgagaag cctgacaaat ggcgagatgg aaagcgtgat ccaactgcgc
     1681 atgttcgatc agcgaaatct ctttgttgtt ctcactggcg gcgagcattt tttcataaca
     1741 gcgggttatc acaacaaaat cagcctgcgt tcccagcgtt gccgccagcc ttgccgattc
     1801 gccctccagt aatgcgcgaa cgtcgagcag atcgtacagc gttcgcggct gcgtactgaa
     1861 cagatggatc agcgggctgg tgtcctgcac ccgattaagc cgtgcgacac gagaatcgcg
     1921 accctgcgcc gtttcaataa tcccgcgccc gcgcagcacg gtcagccctt cacgcagtgc
     1981 ggagcgtgag aagccgagct tttcacacag tcgacgttcc gagggaagtg gctgaccgac
     2041 cttcagtacg ccgtcgataa ttaaccgttc gatactctct gcaaccactt cgcaaatagg
     2101 gcgacgttca tctttcattc ccggacctcg tgcacaggta ggaccaattt ttctgttctt
     2161 aactcgcaaa acacgcacat cacgtaagtg attatgttac atcaatttaa cattgagtta
     2221 accaaggcaa ggtcacagag ctggaaaaaa aatggtctga ccggtagcta aagagataga
     2281 cgaaaacgaa aagcccgctt aataactgtt cacagaagca gcgcgcaaaa atcagctgcc
     2341 acacaacaca acaaagcgaa gcctactcat gagcatcttg tacgaagagc gtcttgatgg
     2401 cgctttaccc gatgtcgacc gcacatcggt actgatggca ctgcgtgagc atgtccctgg
     2461 acttgagatc ctgcataccg atgaggagat cattccttac gagtgtgacg ggttgagcgc
     2521 gtatcgcacg cgtccattac tggttgttct gcctaagcaa atggaacagg tgacagcgat
     2581 tcttgctgtc tgccatcgcc tgcgtgtacc ggtggtgacc cgtggtgcag gcaccgggct
     2641 ttctggtggc gcgctgccgc tggaaaaagg tgtgttgttg gtgatggcgc gctttaaaga
     2701 gatcctcgac attaaccccg ttggtcgccg cgcgcgcgtg cagccaggcg tgcgtaacct
     2761 ggcgatctcc caggccgttg caccgcataa tctctactac gcaccggacc cttcctcaca
     2821 aatcgcctgt tccattggcg gcaacgtggc agaaaatgcc ggcggcgtcc actgcctgaa
     2881 atatggtctg accgtacata acctgctgaa aattgaagtg caaacgctgg acggcgaggc
     2941 gctgacgctg ggatcggacg cgctggattc acctggtttt gacctgctgg cgctgttcac
     3001 cggatcggaa ggtatgctcg gcgtgaccac cgaagtgacg gtaaaactgc tgccgaagcc
     3061 gcccgtggcg cgggttctgt tagccagctt tgactcggta gaaaaagccg gacttgcagt
     3121 tggtgacatc atcgccaatg gcattatccc cggcgggctg gagatgatgg ataacctgtc
     3181 gatccgcgcg gcggaagatt ttattcatgc cggttatccc gtcgacgccg aagcaatttt
     3241 gttatgcgag ctggacggcg tggagtctga cgtacaggaa gactgcgagc gggttaacga
     3301 catcttgttg aaagcgggcg cgactgacgt ccgtctggca caggacgaag cagagcgcgt
     3361 acgtttctgg gccggtcgca aaaatgcgtt cccggcggta ggacgtatct ccccggatta
     3421 ctactgcatg gatggcacca tcccgcgtcg cgccctgcct ggcgtactgg aaggcattgc
     3481 ccgtttatcg cagcaatatg atttacgtgt tgccaacgtc tttcatgccg gagatggcaa
     3541 catgcacccg ttaatccttt tcgatgccaa cgaacccggt gaatttgccc gcgcggaaga
     3601 gctgggtggg aagatcctcg aactctgcgt tgaagttggc ggcagcatca gtggggaaca
     3661 tggcatcggg cgagaaaaaa tcaatcaaat gtgcgcccag ttcaacagcg atgaaatcac
     3721 gaccttccat gcggtcaagg cggcgtttga ccccgatggt ttgctgaacc ctgggaaaaa
     3781 cattcccacg ctacaccgct gtgctgaatt tggtgccatg catgtgcatc acggtcattt
     3841 acctttccct gaactggagc gtttctgatg ctacgcgagt gtgattacag ccaggcgctg
     3901 ctggagcagg tgaatcaggc aattagcgat aaaacgccgc tggtgattca gggcagcaat
     3961 agcaaagcct ttttaggtcg ccctgtcacc gggcaaacgc tggatgtgcg ttgtcatcgc
     4021 ggcattgtta attacgaccc gaccgagctg gtgataaccg cgcgtgccgg aacgccgctg
     4081 gtggcgattg aagcggcgct ggagagcgcc gggcaaatgc tcccctgcga gccgccgcat
     4141 tatggtgaag aagccacctg gggcgggatg gtcgcctgcg ggctggcggg gccgcgtcgc
     4201 ccgtggagcg gttcggtccg cgattttgtc ctcggcacgc gcatcattac cggcgctgga
     4261 aaacatctgc gttttggtgg cgaagtgatg aaaaacgttg ccggatacga tctctcacgg
     4321 ttaatggccg gaagctacgg ttgtcttggc gtgctcactg aaatctcgat gaaagtgtta
     4381 ccgcgaccgc gcgcctccct gagcctgcgt cgggaaatca gcctgcaaga agccatgaac
     4441 gaaatcgccc agtggcaact ccagccgcta cccattagtg gcttatgtta cttcgacaat
     4501 gcgttgtgga tccgccttga gggcggcgaa ggatcggtaa aagcggcgcg tgaactgctg
     4561 gggggcgaag aggttgcggg tcagttctgg cagcaattgc gtgaacaaca actgccgttc
     4621 ttctcgttac caggtacctt atggcgcatt tcattaccca gcgatgcgcc gatgatggat
     4681 ttacccggcg agctactgat cgactggggc ggagcgttac gctggctgaa atcgacagcc
     4741 gaggacaatc aaatccatcg catcgcccgc aacgctggcg gtcatgcgac ccgctttagt
     4801 gccggagatg gtggctttgc cccgctatcg gctcctttat tccgctatca ccagcagctt
     4861 aaacagcagc tcgacccttg cggcgtgttt aaccccggtc gcatgtacgc ggaactttga
     4921 ggagcaggct atgcaaaccc aattaactga agagatgcgg cagaacgcgc gcgcgctgga
     4981 agccgacagc atcctgcgcg cctgtgttca ctgcggattt tgtaccgcaa cctgcccaac
     5041 ctatcagctt ctgggcgatg aactggacgg gccgcgcggg cgcatctatc tgattaaaca
     5101 ggtgctggaa ggcaacgaag tcacgcttaa aacacaggag catctcgatc gctgcctcac
     5161 ttgccgcaat tgtgaaacca cctgtccttc tggtgtgcgc tatcacaatt tgctggatat
     5221 cgggcgtgat attgtcgagc agaaagtaaa acgcccactg ccggagcgaa tactgcgcga
     5281 aggattgcgc caggtagtgc cgcgtccggc ggtcttccgt gcgctgacgc aggtagggct
     5341 ggtgctgcga ccgtttttac cggaacaggt cagagcaaaa ctgcctgctg aaacggtgaa
     5401 agctaaaccg cgtccgccgc tgcgccataa gcgtcgggtt ttaatgctgg aaggctgcgc
     5461 ccagcctacg ctttcgccca acaccaacgc ggcaactgcg cgagtgctgg atcgtctggg
     5521 gatcagcgtc atgtcagcta acgaagcagg ctgttgtggc gcggtggact atcatcttaa
     5581 tgcgcaggag aaagggctgg cacgggcgcg caataatatt gatgcctggt ggcccgcgat
     5641 tgaagcaggt gccgaggcaa ttttgcaaac cgccagcggt tgcggcgcgt ttgtcaaaga
     5701 gtacgggcag atgctgaaaa acgatgcgtt atatgccgat aaagcgcgtc aagtcagtga
     5761 actggcggtc gatttagtcg agcttctgcg cgaggaaccg ctggaaaaac tggcaattcg
     5821 cggcgataaa aaactggcct tccactgccc gtgtaccctg caacatgcgc aaaagctgaa
     5881 cggcgaagtg gaaaaagtgt tgcttcgtct tggatttacc ttaacggacg ttcccgacag
     5941 ccatctgtgc tgcggttcag cgggaacata tgcgttaacg catcccgatc tggcacgcca
     6001 gctgcgggat aacaaaatga atgcgctgga aagcggcaaa ccggaaatga tcgtcaccgc
     6061 caacattggt tgccagacgc atctggcgag cgccggtcgt acctctgtgc gtcactggat
     6121 tgaaattgta gaacaagccc ttgaaaagga ataaaaaaat gaaaactaaa gtcattctta
     6181 gccagcaaat ggcgagtgca attattgccg caggtcagga agaggcgcag aaaaataact
     6241 ggtctgtttc cattgctgtt gccgatgacg gcggtcatct gctggcgtta agtcgcatgg
     6301 acgattgcgc gccgattgcg gcttacatct cccaggagaa agcgcgtacc gccgcgctgg
     6361 ggcgtcgtga aactaagggc tatgaagaga tggtgaacaa cggacgtacc gcgttcgtga
     6421 ctgcgccgtt actgacgtcg ctggaaggtg gcgtaccggt tgttgttgat gggcaaatta
     6481 ttggtgccgt gggcgtttct ggtttaaccg gagcacagga tgcgcaggtc gcgaaagcgg
     6541 cagcagcggt gttggcgaaa taagcgaaaa cgaggagata aacaatgagt caaaccataa
     6601 cccagggccg tttacgcatt gacgccaatt ttaaacgttt tgtggatgaa gaagttttac
     6661 cgggaacagg gctggacgct gcggcgttct ggcgcaattt tgatgaaatc gttcacgatc
     6721 tggcaccaga aaatcgtcag ttgctggcag agcgcgatcg cattcaggcg gcgcttgatg
     6781 agtggcatcg cagcaatccg gggccggtaa aagataaagc ggcctataaa tctttcctgc
     6841 gtgaactggg ctacctggtg ccgcaaccgg agcgcgtgac ggtggaaacc acgggcattg
     6901 acagcgaaat caccagtcag gcggggccac agctggtggt tccggcaatg aacgcccgct
     6961 acgcgctgaa cgcggcgaac gctcgctggg gctcactgta cgatgcgtta tacggcagcg
     7021 acatcatccc gcaggaaggg gcgatggtca gcggctacga tccgcaacgc ggtgagcagg
     7081 ttatcgcctg ggttcggcgt ttcctcgatg aatctctacc gctggaaaac ggcagctatc
     7141 aggatgtggt ggcgtttaag gtggtcgata aacaattacg catccagttg aaaaatggta
     7201 aagaaaccac gttacgtacc ccggcgcagt ttgtcggtta ccgtggcgat gccgctgcgc
     7261 cgacctgcat tttgctgaaa aataacggcc tgcatattga actgcaaatt gatgccaacg
     7321 ggcggattgg caaagacgat ccggcgcaca tcaacgatgt tatcgtcgaa gcggccatca
     7381 gtaccattct cgactgcgaa gattcggtcg cggcggttga tgcggaagat aaaatcctgc
     7441 tgtaccgcaa tctgctgggc ctgatgcagg ggactctgca agagaaaatg gataaaaacg
     7501 gtcggcaaat cgtgcgtaaa ctgaatgatg atcgtcagta caccgccgcc gatggctctg
     7561 aaatttctct gcacggacgc tcgttgctgt ttatccgcaa cgtgggtcat ttgatgacca
     7621 ttcctgtgat ttgggacagc gaaggcaatg aaatcccgga aggcattctt gatggcgtca
     7681 tgactggcgc gattgccctc tatgatttaa aagtgcagaa aaactcgcgc actggcagcg
     7741 tctatattgt gaaaccgaaa atgcacggtc cgcaggaagt ggcgttcgcc aacaaactgt
     7801 ttacccgcat tgagacaatg ctcggtatgg caccgaatac cctgaaaatg ggcattatgg
     7861 atgaagaacg tcggacctcg ctgaacttgc gtagctgtat cgctcaggcg cgtaaccgcg
     7921 tggcgttcat caataccggt ttcctcgacc gtaccggcga tgaaatgcat tcggtgatgg
     7981 aagctggccc gatgctgcgt aaaaaccaga tgaaatcgac gccttggatc aaagcctacg
     8041 aacgtaataa cgtgctttcc ggcctgttct gtgggctgcg cggtaaagcg caaatcggta
     8101 aaggcatgtg ggcaatgccg gacctgatgg cagacatgta cagccagaag ggcgaccaac
     8161 tgcgtgccgg ggcaaacaca gcctgggttc cgtcgccaac tgccgctacg ctccatgcga
     8221 tgcactacca ccaaaccaac gtacagagcg tgcaagccaa cattgcccag accgagttca
     8281 atgctgaatt tgaaccgctg ctggacgatc tgctgactat tccggtggct gaaaacgcta
     8341 actggtcggt ggaagagatc caacaagagc tggataacaa cgtgcagggg attctggggt
     8401 acgtggtgcg ctgggtggag caggggattg gttgttcaaa agtgccggat attcacaatg
     8461 tggcgctgat ggaagaccgc gcaacgctgc gtatctccag ccagcatatc gccaactggt
     8521 tacgtcacgg tattctgacc aaagaacagg tgcaggcgtc gctggagaat atggcgaaag
     8581 tggttgatca gcaaaacgct gacgatccgg cttatcgtcc gatggcgggg aatttcgcta
     8641 actcgtgtgc ttttaaagct gccagcgatt taatcttcct cggcgtgaaa cagccaaacg
     8701 gctataccga accgttatta cacgcctggc gtttacgcga aaaagaaagt cattaatagc
     8761 gtcaaaaaag ccccggcaag ttttattgtc ggggctatga tagtaactgc ggtagtacat
     8821 ttatcccgga aataaccgga gcgattatca gggtttaaca atattaaata cccgtagcgc
     8881 attactagcg ggcctaaaac aacaatatgg acatcttaac gatactctgc gcctgcgtgt
     8941 gcggaacaga aacccttacc cgatatactt gttatgccag aggtagcaat tgcgttagcg
     9001 gcgtactgtt gctgtaaagt ataacgtcta tattttacag ttcagtcctc ttttgcttat
     9061 tacaacctta agtatatcaa gcatataaag ataataagag actgaacaat atggttacct
     9121 ggacccaaat gtatatgcca atgggaggac tggggctatc cgctctggtc gccctgatcc
     9181 cgataatatt cttcttcgtt gcactcgcgg tattacgtct gaaaggacat gtcgctggag
     9241 caataaccct tatattatct atcctgattg caatattcgc ctttaaaatg ccgattgata
     9301 tggcttttgc tgctgcgggc tatggcttta tttatggatt atggcctatt gcgtggatta
     9361 ttgtcgcggc ggtgttcctc tataaattaa ccgttgccag cggtcagttc gatattatcc
     9421 gcagctcggt tatctccatc accgacgatc agcgtttgca ggtgttactg attggtttct
     9481 cctttggtgc gttgctggaa ggagcggctg gctttggtgc gccggtggcg attaccggtg
     9541 cgctgctggt gggcctgggc ttcaaaccgt tatacgcggc ggggctgtgt ctgattgcca
     9601 acactgcgcc agtggcgttt ggtgcgttgg gcgtgccgat tctggtcgcc ggtcaggtaa
     9661 cgggaatcga tccgttccac attggcgcaa tggcgggacg tcagttaccg ttcctgtcgg
     9721 ttcttgtgcc gttctggctg gtagcaatga tggacggctg gaaaggggtg aaagagacgt
     9781 ggccagcggc gctggttgct gggggaagct tcgctgtcac tcagttcttt acctctaact
     9841 atattggtcc ggaactgccg gatattactt cggcgctggt gagtatcgtc tcactcgctt
     9901 tattccttaa agtctggcgg ccgaaaaata ccgaaacggc aatcagcatg gggcaatccg
     9961 caggtgcgat ggtggtaaat aagccatctt ctggcggtcc cgtgccttca gaatatagtc
    10021 tggggcaaat cattcgtgcg tggtcaccgt ttttaatctt aacggtgctg gtcaccatct
    10081 ggaccatgaa gccgtttaaa gcgttatttg ctccgggcgg cgcgttttat tcactggtga
    10141 ttaatttcca gatccctcat ttgcatcaac aagtgttgaa agcggcaccc attgtcgccc
    10201 aaccaacgcc aatggatgcg gtgtttaaat tcgaccctct ctcggctggc ggcaccgcta
    10261 tttttattgc ggcgattatc tctatcttca tcctcggcgt gggcatcaag aaagggattg
    10321 gcgtctttgc cgaaacgcta attagtctga agtggccgat attgtcgatt ggcatggtgc
    10381 tggcgttcgc cttcgtcacc aactattccg gcatgtccac cacgctggcg ctggtactgg
    10441 caggtacagg cgtgatgttc ccgtttttct caccgttcct cggctggctg ggggtattcc
    10501 ttaccggctc ggacacttct tcaaacgccc tgtttggttc actgcaatcg actacggcgc
    10561 agcaaatcaa cgtctctgac actctgctgg tggcagcaaa taccagcggc ggcgtaactg
    10621 gcaagatgat ctccccgcaa tctatcgccg tggcctgcgc cgcgacgggc atggtgggcc
    10681 gagaatctga actattccgc tacaccgtga agcacagtct gatttttgcc agcgttatcg
    10741 gcgttatcac gttgttgcag gcgtatgtgt ttactggaat gttggtctcg taaatcaata
    10801 atgccggatg atcgttaata tcatccggct tcatttacat tattctgtct tattaattga
    10861 aaattatttc tcgctttatt atccatgcgg caataatgtt ttgtgttcag ataacctgat
    10921 aaataattaa gagcgtgaga acatctattt ttttatcacg caaaacaagt ctgtattaat
    10981 tatgttcgct tgatgagcaa acaccgttct atctgttgct tttgcaatct taatcgttaa
    11041 aactaccaac gaatttatat agttgcgttg tgaaatgttg ttaacttttt gtttattgaa
    11101 atttcgtaaa aatagccagc cagacatcaa ataggggtta ttcttataaa tttcacctgt
    11161 tttctatcaa tgatgtcgtt ttcttaagaa tggaggaaat t
//
LOCUS       DAZFXT010000143        11182 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.143, whole genome shotgun sequence.
ACCESSION   DAZFXT010000143 DAZFXT010000000
VERSION     DAZFXT010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11182
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.143"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..826
                     /gene="gadX"
                     /locus_tag="ACHKW0_004105"
     CDS             2..826
                     /gene="gadX"
                     /locus_tag="ACHKW0_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HFO7476849.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1194..1922
                     /gene="gadW"
                     /locus_tag="ACHKW0_004106"
     CDS             1194..1922
                     /gene="gadW"
                     /locus_tag="ACHKW0_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HFO7476850.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2285..5398)
                     /gene="mdtF"
                     /locus_tag="ACHKW0_004107"
     CDS             complement(2285..5398)
                     /gene="mdtF"
                     /locus_tag="ACHKW0_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
                     /protein_id="HFO7476851.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLCTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5423..6532)
                     /gene="mdtE"
                     /locus_tag="ACHKW0_004108"
     CDS             complement(5423..6532)
                     /gene="mdtE"
                     /locus_tag="ACHKW0_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HFO7476852.1"
                     /translation="MLTACDDKSAENAAAMTPEVGVVTLSPGSVNVLSELPGRTVPYE
                     VAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELNSAKGSLAKALSTASNA
                     RITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAVEQATINLQYANVTSPI
                     TGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQDFLRMKEEVASGQIKQV
                     QGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIFPNPNGDLLPGMYVTAL
                     VDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKAIGDQWVVTSGLQ
                     AGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6640..6918
                     /locus_tag="ACHKW0_004109"
     CDS             6640..6918
                     /locus_tag="ACHKW0_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476853.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6919..7446)
                     /gene="gadE"
                     /locus_tag="ACHKW0_004110"
     CDS             complement(6919..7446)
                     /gene="gadE"
                     /locus_tag="ACHKW0_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HFO7476854.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8245..8817)
                     /gene="hdeD"
                     /locus_tag="ACHKW0_004111"
     CDS             complement(8245..8817)
                     /gene="hdeD"
                     /locus_tag="ACHKW0_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HFO7476855.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            9072..9404
                     /gene="hdeA"
                     /locus_tag="ACHKW0_004112"
     CDS             9072..9404
                     /gene="hdeA"
                     /locus_tag="ACHKW0_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HFO7476856.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            9520..9846
                     /gene="hdeB"
                     /locus_tag="ACHKW0_004113"
     CDS             9520..9846
                     /gene="hdeB"
                     /locus_tag="ACHKW0_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HFO7476857.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            9910..10557
                     /gene="yhiD"
                     /locus_tag="ACHKW0_004114"
     CDS             9910..10557
                     /gene="yhiD"
                     /locus_tag="ACHKW0_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HFO7476858.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(10599..11129)
                     /gene="dctR"
                     /locus_tag="ACHKW0_004115"
     CDS             complement(10599..11129)
                     /gene="dctR"
                     /locus_tag="ACHKW0_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7476859.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT         3076 a         2519 c         2576 g         3011 t
ORIGIN      
        1 tatgcaatca ttacatggga attgtctaat tgcgtacgca agacataaat atattctcac
       61 catggttaat ggtgaatatc gctattttaa tggcggtgac ctggtttttg cggatgcaag
      121 ccaaattcga gtagataagt gtgttgaaaa ttttgtatta gtatcaaggg atacgctttc
      181 attatttctg ccgatgctca aggaggaggc attaaatctt catgcacata aaaaaatttc
      241 ttcattactc gttcatcact gtagcagaga tattcctgtt tttcaggaag ttgcgcaact
      301 atcgcagaat aagaatcttc gctatgcaga aatgctacgt aaaagagcat taatctttgc
      361 gttgttatct gtttttcttg aggatgagca ctttataccg ctgcttttga acgttttgca
      421 accgaacatg cgaacacgag tttgtacggt tatcaataat aatatcgccc atgagtggac
      481 actagcccga atcgccagcg agctgttgat gagtccaagc ctgttaaaga aaaaattgcg
      541 cgaagaagag acatcatatt cacagttgct tactgagtgt agaatgcaac gtgctttgca
      601 acttattgtt atacatggct tttcaattaa gcgagtcgca gtgtcctgtg gatatcacag
      661 cgtgtcgtat ttcatttacg tctttcgaaa ttattatggg atgacgccca cagagtatca
      721 ggagcgatcg gcgcagggat tgccgaaccg tgactcggcg gcaagtattg ttgcgcaagg
      781 aaatttttac ggcactgacc gttctgcgga aggaataaga ttatagaatt ttactcagac
      841 ataaaaaaaa cccggcatag gggaccggga agaggatagt ctgccgtctc cagactaata
      901 aaccgttata acactccctg ttggcacggg aaactttgtg ctctcagtaa gttaaatata
      961 acttttactg gaaataagat cagccatttt tttataaaca taagctatac gcagtgcgaa
     1021 aatatattcg tgctgcattt acttattatc aattaactgt tatgcaaaac tactttgtgg
     1081 ataaattttg gtcctaccaa atctggcagt tttttcgcta agaaacagtc tggcaacatt
     1141 tcattagtat actgaaattg aaataatcgc agtatgaaat ataagggata atcatgactc
     1201 atgtctgctc ggtgatcctc attcgtcgtt cattcgatat ttatcatgaa cagcataaaa
     1261 tatcgctgca taacgagagt atcgtgctgc tggagaaaaa tttggcagac gattttgcgt
     1321 tttgttcacc ggatacgcga cgactggata tcgatgagct gacagtttgc cattacttac
     1381 aaaatattcg tcagctacca cgcaatttag ggttacatag caaagaccgt ctattaatta
     1441 accagtcacc ccccatgccg ctggtgacgg cgatttttga tagcttcaat gaatccgggg
     1501 taaattcacc gatactgagc aatatgctct acctttcctg tttatcgatg ttttctcata
     1561 agaaagaact gatcccctta cttttcaata gcatcagcac tgtttcagga aaagttgaac
     1621 gccttattag ctttgatatc gccaaacgtt ggtatctgcg cgatatcgcg gaaagaatgt
     1681 ataccagcga gagtctcatc aaaaaaaagt tgcaggatga aaatacctgt ttcagtaaaa
     1741 tattactcgc ttccaggatg tcgatggcca gacgattact cgagttacgt caaattcctc
     1801 tgcatactat tgcagaaaaa tgtggctata gcagtacgtc gtactttata aacacatttc
     1861 gacaatatta tggtgtaacg ccacatcagt ttgcgcaaca ttcgccaggt accttttcct
     1921 gacatatttt gcatttgaat attggtcagg atctcacacc tgcttcatgt gaaactcttc
     1981 cctgatgatt tctgccgggc taccggctag ttctctttcg cagtttgcca ggcggtatag
     2041 gtattttcag gaggaaaaga ctcggcatgt ttgggattat taagctgaca attcatacga
     2101 ttacgggaat gtgggtcagt attgtgctgt tcaaattgat gacgaatggc tggagtggtt
     2161 tctactttca gtgctgtgta ctaagcctgg tattcctgac ggttagctgg ttgttgagtg
     2221 gtgaatggtt agcaggaaag agtaaggctg aaccttcacg ttcaacctta ctctcattta
     2281 cacgttacgc ttttttaaag cgggcaaaga gatgttccac tacaacgaaa aagaccggaa
     2341 cgaagtaaat tgccagcact gttgcggcaa acatcccgcc catcacgccg gtacctaccg
     2401 cgttttgcgc gccagaaccg gcaccatgac tgataaccag cggcagcacg ccgagaataa
     2461 aggccagaga ggtcatcagg attgggcgta aacgcatccg cgccgcttcg atgattgcct
     2521 ctatcggcgt tttcccttct ttctgcatca tctcaacggc aaattcgaca atcaggatgg
     2581 cgttcttggc ggaaagcccg atggtggtca gcaaaccaac ctggaagtag acgtcattac
     2641 ttaagccgcg cagatcggtg gccagtaatg cgccaacgac gcctaacgga acaaccaaca
     2701 tcaccgagaa cggaattgac cagctctcat agagtgcggc gagggcgagg aacaccacga
     2761 ccagtgaaat ggcatacagt gcaggagcct ggtttgagga taacgcttcc tgatacgaca
     2821 gtccggtcca tgagtagccg acgcctgccg gaagtttagc gaccaggtct gccataaatt
     2881 tcatagcgtc accggtactt ttcccggccg ccgcttcacc taaaatctcc attgacggga
     2941 tgccgttgta gcgttccaat cgcggtgaac cataggtcca ttcggtagac gagtaggcag
     3001 aaagcggtgc catcgtgcca gaggcgttgc gtacatacca ttggttgatg ttatccggca
     3061 acatacggaa cggcgtgcct gcctggacat acactttttt cacccgcccc tggttgagga
     3121 agtcgttcac gtagctgctg ccgaaggcgg tggaaattgt ctggttgata tcagacagcg
     3181 ccacacccat tgcctcagct ttcgcggcgt tgacgttcac tttgaacatc ggcgtatctt
     3241 ccaggccgtt cgggcgtacc ccggtgacct ggtcaggcga ttgcgctgcc agtgataaca
     3301 gctcgtttcg tgcctgggtt agtttttcgt gccccaggtt accgttgtcc agcagttcca
     3361 tatcaaaacc tgacgcggta cccagttcag ccaccgcggg taagttgaac gggaagacga
     3421 cggctttatt gatactgctt aacgcaatca ttgcccgctg aatgatcgcg gtaaccgagt
     3481 tttcctcacc gacacgttca gaccacggct tgagactgat aaacgccagg ccgttgtttt
     3541 gcccctggcc gctgaagcca aagccgccaa cggtaaacac cgactgaaca ttatctttct
     3601 cttttgtaag ataataatcc gtcacctgtt gcagcacttt cgtggtgtta accatggtgg
     3661 caccggaagg taactgcgcg gtggtcataa ataccccctg atcctcttct ggtaagaaag
     3721 aggtcggtgt acacaggaac agcaccgcca tcccggcgca aatcagcagg tagaccacca
     3781 tgtagcgacc ggtacaacgc aacagcgagc gggtgctatc ggtatagtgc tgagttgatt
     3841 tttcaaacag cgtgttgaag cgtgcgaaca gggcgttagg tttgtgaccg ccttccggcg
     3901 cggctttcag aatggtggcg cacagggcag gggtcaggct cattgccaca aatactgaaa
     3961 gcagcatgga ggagatcagc gtgatggaga actggcggta gatctccccg gttgcgccgc
     4021 tcataaaggc catcggcata aacactgcgg aaagaacaac agcaataccg accagcgcac
     4081 gttggatctg ccccatcgat ttatgcgtcg cttccttcgg tggtagctta tcttccgcaa
     4141 tgacacgctc gacgttctcc accaccacga tggcgtcatc caccagtagc cctatcgcca
     4201 gcaccatccc gaacatagtt aacgtgttga tggtgaaacc gaccgccgac aagatcgcaa
     4261 acgtcccgag aataaccacc ggtacggcaa tcgtcgggat gattgtggca cggaaattct
     4321 gcaaaaacag atacatgacc aggaagacga ggatgatagc ctcaaccagt gttttaaaaa
     4381 cttcctgaat agaaatttcg ataaacggcg tggtgtcgta aggataaacc gtcttcagac
     4441 ttgcggggaa ataggctgat aagcggttca gctcctcttt gactgcccgc gaggtatcca
     4501 gggcgtttgc tccggtagcc agtttgatgg cgatcccggc agcaggtttg ccgttatagc
     4561 gcgccacggt ggaataatct tccgctccaa gttcgacgcg ggcgacatca cgcagcagca
     4621 cttgcgaacc atcttgctga actttcaaca ggattttgcc aaattcttcc ggcgtttgca
     4681 gacgcgtctg cacaatgatc gaggcgttta gttgctggtc tgccgcctgt ggcatgccac
     4741 ccagttgacc accggaaatc tggttgtttt gcaccttaat ctgggaaata acatcggaag
     4801 gtaccagatt gtatttattg agtttttgcg ggtccagcca gatacgcatg gcgtactcgg
     4861 aaccaaagag ctgtacgcta ccaacgcccg cggtacggct tagcgggtct ttgatattag
     4921 acgctacata gtccgcgata tcgtactggt tgaggctgcc gttatcagaa ataaacgccg
     4981 ctaccatcag aatattactg ctcgacttat caacgctaat cccctgctgc tgcaccgctt
     5041 caggtaatga aggcatagcg agttgcagtt tattttgcac ctgaacctgt gcgatatcag
     5101 gagatgtccc agtctcgaag gtcagagtga tagaggcatt acccgccgca tcactggttg
     5161 aagacatgta catcaggcca tcaagcccat tcatattttg ctcaatcacc tgagtgaccg
     5221 agtcttctac cgtttgcgca tcggcacctg gataggtagc gctgacggta atggtcggtg
     5281 gcgcaatctg cggatactgc gcaaccggta agttcatgat cgccagacca cctgcaagca
     5341 tcataataat ggcaagtacc caggcaaaaa ccgggcgatc aataaaatag ttagccatga
     5401 aagtcccctt aagcctgcaa cgttattgtt tcgattcggt gctggcgttt tcctggctgg
     5461 aggaaattgc tcgtgctttg atacccggac gaatgcgttg caaaccggaa acgatcaccc
     5521 gatcgccagc ctgcaagcca gaggtgacaa cccactggtc gccgatggct ttgctggctt
     5581 caatttcgcg tagctgcaca acatcgtctt tatccagaat gagcgccgtt gctttaccct
     5641 gggcgttgtg ggtgacgcct tcctgcggca ccagtaatac attctggcgg ctaccttcat
     5701 ccactaatgc cgtgacgtac atgccaggca gcaagtcacc atttgggttg gggaaaatcg
     5761 cccgtaacgt cacggagccc gtggtttcat ccaccgtcgg gtcggagaat ttcagcgtgc
     5821 cggtctggct gtagcgttta ccattttcca gattgagctg tactggcgta ctgccctgaa
     5881 cctgtttgat ttgcccactg gcgacctctt ctttcatgcg taagaaatcc tgcaccgact
     5941 gcgtgagatc gacataaatc gggtccagac gttgtacggt aaccagcgaa tctgcctgat
     6001 tagcggtaac gagtgcgccg acggtcaccg acgatttccc gctgacgccc gtaatcggcg
     6061 aggtgacatt cgcgtattgc agattgatcg tcgcctgttc aacagccgct ttggcgacgg
     6121 tgacattggc ttctgcttca ttcaactggg tgcgcgcggt gtcgtaatcc tgacggctaa
     6181 cgtagttggt cttcagcaac gatgcctggc ggttaaaggt gatgcgggca ttgctggcgg
     6241 tagagagcgc tttcgccagc gagcctttgg cggagtttag ctcggcctgt aaaggtgcag
     6301 gatcaatctg atacagcgaa tcgccctggt tcactttatc gccttcgata aagttgcgtt
     6361 taatgataat accgcccacc tggggacgta tctcggcaac ttcataagga acggttctac
     6421 cgggcaattc gctcaacaca ttgaccgaac cgggggagag tgtgacgaca ccgacctcag
     6481 gcgtcatggc ggcggcgttt tccgccgatt tgtcatcgca ggcggtgagc atcgcgccgc
     6541 agaaaaacaa cggtattaac agctttcttc ttctgttcat tttagtccct gaaaattctt
     6601 gagaaataac ggttctttaa attgcgcgcg aggagagata tgagggattt acagaccagc
     6661 ggcatagtgg ggttgtcagc cagtaaggtg ggatacaggc acagtgatcg acatggtgag
     6721 gtcaacgaca aaaaaaatgt tgccgttctg ccaacagttc ctgccagcat tcgggcaacg
     6781 aagggttcta ctggtggata cacataccag gggaataaag tcgttttagc ctctccttta
     6841 gtaatcaccg ggggtaatga aatcagcatc tgcatccctc gtcatgccag ccatcaattt
     6901 cagttgctta tgtcctgact aaaaataaga tgtgataccc agggtgacga tgtcgctcat
     6961 acgtcttagt ttcagcttgt acataatgtt gaatttctga atctttaatg tttgttgcga
     7021 aatattgagc gtcgacgtga tattgctttc atttttattt ttcagaatca gttgcataat
     7081 tttgaactga ttatgtgaaa aacataattg tgaattctta tggggcaagt gtttaccata
     7141 agtaatctca agaagatttt tgatactttc tttgcggcta acaaaataaa taggcgcttt
     7201 agcttttagt ttactgatgt gataaggtgc caaaacaata attctctcgg catctaattt
     7261 ctccagaaat ttaatcgctt cttcatcaag gatatgattg tgataggtat caatgataac
     7321 cttgaagggt ttattgccta cttttttgat ctctgacagg gaattgattt ttatcatttc
     7381 gtgattatct ttcaactgcc aaaagccctg taaaagaaaa gaatctttcg tcatgagaaa
     7441 aatcataact tgctccttag ccgttatcgt ttcgaacatg tcatcctttt cattatttac
     7501 atccttgtcc gaatcgttgt tcaatatagt aaacgcctat aagaactttg cccttcgtga
     7561 actaatacgt ctattaacac taatcaatac ttcagattta tccatcacgc actccatata
     7621 ttgatcctaa gtaagattcc tggttgttat caatttgtaa atatcaaaca aaaagttaac
     7681 gtttgcggat tactgcccaa gaataagtta tcaataacca gtttgtgatc tctgaagaat
     7741 attactaaag ttaaaatgta atccgattta aatatcgagt ctccttgttt cgacttaagc
     7801 tggcaattgg attgccagct ttcttttaac gcattgaaat tacctgagta aataacgcat
     7861 tcatgtcttc aggtttttat atagcgtctg gagacacgga tatttatgca atgtaatcaa
     7921 cgccagttgc aaaaaaatga acccggatta cttatttaac cgcccatagc atcaacgctt
     7981 acggaactct ttgttcactt tcccacagca cttttaaaac attaaacctg actacggaaa
     8041 atatcagcca tgataatgtt tgaaatggct aatttgccat ggagtgaaaa aaattagatg
     8101 aaattcagta ggttgaaatg atcactagca ggtaattatt tcaatgatag tgcgcaattg
     8161 atctacaaca ctgcgtagcg gagagagtat taatcggatc atagtcacat caagtgacta
     8221 tgatccgggt gacaaccggg ataattattg ctgcttaacg aacaaactgg cgaagctgaa
     8281 caggctggcg gcgctaaata tcagttcaat tcccaccagt gtggaaacca gcgttacaga
     8341 caccatcggc gttgcaccaa ggaatatcca ggcaatgacg atatccagca caccaataac
     8401 gagctgtagc cagctgcctt tcattgaacg ctgacgatac caactcatca ggcgaataac
     8461 ccctgcaaca cagaacaaac cggcaataaa tgccgcaatg gcaaaaatgc ccagctccgg
     8521 tgcgcggatg aagaaatagc cgatcaataa ataggcgact gcgacgagga aaccggataa
     8581 taccggccag aaattatgac tgcggttgct gaataacccg acaataagcg caatacccga
     8641 gcagattaat aatgcaccca ctactgtgct taaaatatcg ccagagacga acgggaaact
     8701 gatacacagc aacccgacga taaacagcag cacggcaata aactggattg ctctgcgatg
     8761 ctttttcagc atctccagat caaacttcaa aattgttgcc ttatctatat ataacataga
     8821 accaccctat aaaattaaga agaaaatccc ctgctatcaa tctatgccaa aaacgcgtct
     8881 aagaatgcag tcgatttaat aaaaatttcc taattgcagt atctgatgca tctgtaactc
     8941 attgtattga aataaaaata tctgattttg atattttcca tcaacatgac atatacagaa
     9001 aaccaggtta taacctcagt gtcgaaattg attcgtgacg gctctttcac tttatagttg
     9061 aggatattac gatgaaaaaa gtattaggcg ttattcttgg tggtctgctt cttctgccag
     9121 ttgtgagcaa tgcagcggat gcgcaaaaag cagctgataa caaaaaaccg gtcaactcct
     9181 ggacctgtga agatttcctg gctgtggacg aatccttcca gccaactgca gttggttttg
     9241 ctgaagcgct gaacaacaaa gataaaccag aagatgcggt tttagatgtt cagggtattg
     9301 caaccgtaac cccagctatc gttcaggctt gtactcagga taaacaagcc aactttaaag
     9361 ataaagttaa aggcgaatgg gacaaaatta agaaagatat gtaattccgg gaatgcgtta
     9421 catcgtactt ccttgcatat tgaacaggcc ggaatatctt ctttaaaagc agctattcct
     9481 cctgttcata tataatctct atattgaatg ggttacaaaa tgaatatttc atctctccgt
     9541 aaagcgttta tttttatggg cgctgtagcg gctttgtcac tggtgaacgc acaatctgcg
     9601 ctggcagcca atgaatccgc taaagatatg acctgccagg aatttattga tctgaatcca
     9661 aaagcaatga ccccggttgc atggtggatg ctgcatgaag aaacagtata taaaggtggc
     9721 gataccgtta ctttaaatga aaccgatctc actcaaattc ctaaagtgat cgaatactgt
     9781 aagaaaaacc cgcagaaaaa tttgtatacc ttcaaaaatc aagcatctaa tgacttgccg
     9841 aattaatgag gtacaagtaa aaaggagtag caagttgagc catcttgctg ctcctttttg
     9901 catttttata tgacagcaga atttattata cgtcttatac tcgcggcaat tgcctgtggc
     9961 gctattggca tggaaaggca aatgcgcggc aaaggagcag ggttacgcac acatgtatta
    10021 attggcatgg gaagcgccct gtttatgatt gtttcgaaat atggttttgc tgacgtgctg
    10081 tctttagatc acgtcggact cgaccccagc cgtattgctg ctcaggtggt gacgggcgtc
    10141 gggtttatcg gtgcaggtaa cattctcgtt cgtaaccaaa atattgtagg tctgacgacg
    10201 gcagcggata tctgggtgac cgccgccata ggtatggtta ttggcagcgg tatgtacgaa
    10261 ctgggtattt atggttcagt gatgaccttg ttggtgctgg aagtcttcca tcaattaacc
    10321 ttccgcctga tgaacaaaaa ttaccattta cagctgacat tagtgaacgg taatacggta
    10381 tcaatgctcg actggtttaa gcagcaaaaa ataaaaacgg atttagtttc gttgcaggaa
    10441 aatgaagatc atgaagtggt cgccattgat atccagcttc atgccacaac gtcgatagaa
    10501 gacctgttgc gattattaaa aggtatggca ggggtgaaag gcgtttcaat tagctaatct
    10561 gtgtggcagc gtagccagac tcaccgtaag cctgaaattc acaccagata atcaatatgc
    10621 tgatggcgta ccagctcatt gatccgctta agattgagga tcatcatgat attgtgttta
    10681 tggcagtaga acgtttttag cgatattttc agtttctcca gaatatcttc cgttgtcatt
    10741 tgattcgcaa tgcagctcag aacctgatgt tgcttgcgcg aaagtgacac agccgcttca
    10801 ggcacagagc agtaactgcc attgatgagt gcgacaaaat caatgatagt gatagtccgc
    10861 ggaacaaagg tcaccggcac tttccccagg cagcgtttaa tattaaaggg ggagaaaata
    10921 atcacatgga caggttttaa tcgttcaatt tgagtgagaa aattagaatg aaatacatta
    10981 ttcattaatg tatcaataat gacgaaggca ttctgatgca acattacgtc gatctcttct
    11041 tcgttctgta tatgaacgac attaccttta ctcagaatgt ttttcatcgc ggtgaagaac
    11101 atcgtatccc tggtaattat aagaaacata accgactcct ggtgcggaca ttaactttat
    11161 aatcagtttg ccaaaggtaa tt
//
LOCUS       DAZFXT010000144        11149 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.144, whole genome shotgun sequence.
ACCESSION   DAZFXT010000144 DAZFXT010000000
VERSION     DAZFXT010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.144"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            242..1162
                     /gene="cbpA"
                     /locus_tag="ACHKW0_004116"
     CDS             242..1162
                     /gene="cbpA"
                     /locus_tag="ACHKW0_004116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HFO7476860.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            1162..1467
                     /gene="cbpM"
                     /locus_tag="ACHKW0_004117"
     CDS             1162..1467
                     /gene="cbpM"
                     /locus_tag="ACHKW0_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HFO7476861.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(1560..2159)
                     /gene="torD"
                     /locus_tag="ACHKW0_004118"
     CDS             complement(1560..2159)
                     /gene="torD"
                     /locus_tag="ACHKW0_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051259 - protein complex oligomerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HFO7476862.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNELENCIATLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFAARCHQYDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(2156..4702)
                     /gene="torA"
                     /locus_tag="ACHKW0_004119"
     CDS             complement(2156..4702)
                     /gene="torA"
                     /locus_tag="ACHKW0_004119"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0050626 - trimethylamine-N-oxide reductase
                     (cytochrome c) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HFO7476863.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGVIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGAVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            complement(4702..5874)
                     /gene="torC"
                     /locus_tag="ACHKW0_004120"
     CDS             complement(4702..5874)
                     /gene="torC"
                     /locus_tag="ACHKW0_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HFO7476864.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            6004..6696
                     /gene="torR"
                     /locus_tag="ACHKW0_004121"
     CDS             6004..6696
                     /gene="torR"
                     /locus_tag="ACHKW0_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HFO7476865.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            complement(6669..7697)
                     /gene="torT"
                     /locus_tag="ACHKW0_004122"
     CDS             complement(6669..7697)
                     /gene="torT"
                     /locus_tag="ACHKW0_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HFO7476866.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHYTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPLVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            8169..8822
                     /locus_tag="ACHKW0_004123"
     CDS             8169..8822
                     /locus_tag="ACHKW0_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EspJ family T3SS effector
                     ADP-ribosyltransferase"
                     /protein_id="HFO7476867.1"
                     /translation="MPIIKNCLLSISNILRNEKTSYSLIKTEQTGKLLNRKITTTPTP
                     AKLLSYRNADLIKENYITEKVLSIFNIKRDFVAVRIQSNQFTDLKNKTIQGHKDTVAK
                     VMDWYNPQKNAFGIMMGTPRRSADIAKEESRNALNFMIMEKDTFNEKILNSNDNLQKK
                     YGTTENSSWVSASVGSLLDKGAKVYPDISCSLRLGEPFIITLPETVRLDVDIHPLKK"
     gene            complement(8835..9533)
                     /locus_tag="ACHKW0_004124"
     CDS             complement(8835..9533)
                     /locus_tag="ACHKW0_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476868.1"
                     /translation="MNEIRSNPVKFLEEHLILNPQRYAHHDETSLITVNITREDGLLR
                     LKETEDDMAGSDYILFTGMRDTDSPDRFDAPAVVSLSNQCISLRRNDISQTIQQSSPL
                     WLTNQQSGCSVLIVRHGLSESGEQSYSMIHMRPRDSNDFVDEFTPALYANTQEILLER
                     DIQRTLANTFPNEHPEALILVPSAEKFIVDGNSIQLIGIGNELGEFDFYRQIYPVTGG
                     EHLVEALMWTHLPA"
     gene            complement(9734..10315)
                     /locus_tag="ACHKW0_004125"
     CDS             complement(9734..10315)
                     /locus_tag="ACHKW0_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO7476869.1"
                     /translation="MPLTSDINSSSFHLGMEVLRAQVAATGRGEFTMGGETVRIEYSP
                     TDGRFLASDGTGGLFTELLLLGFNNGPQALGERMLSILSGSDAGETQSQVTPQDKIYQ
                     CKFSVNTESLQCPSDATRCPIILETPEEGVFVKNSDSSAVCTLFDVDALSRVVNDGSV
                     HPLTRAPITPSMIVKPEECKYDPARGSFIIKDS"
     gene            complement(10384..10521)
                     /locus_tag="ACHKW0_004126"
                     /pseudo
     CDS             complement(10384..10521)
                     /locus_tag="ACHKW0_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309256.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
     gene            complement(10742..10891)
                     /locus_tag="ACHKW0_004127"
                     /pseudo
     CDS             complement(10742..10891)
                     /locus_tag="ACHKW0_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309257.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2898 a         2905 c         2531 g         2815 t
ORIGIN      
        1 taataaataa tgacgcccca gttaaactta aagtgcctgg ttcaactatc aaaaatcgct
       61 cacccttttt cacctgttta aaatatgttc agtaacccat cttgatggcg acctcctctc
      121 cgcgatgatt tcaataacat attctgtgtt ggcatatgaa attttgagga ttaccctaca
      181 cttataggag ttaccttaca ggggttcctt caatttgtgt tgatttacgc gagataacgc
      241 tatggaatta aaggattatt acgccatcat gggcgtgaaa ccgacggacg atctcaagac
      301 aatcaagacc gcctatcgtc gacttgcccg caaataccat cctgatgtca gcaaagaacc
      361 ggatgccgaa gcccgcttca aagaggtcgc tgaagcctgg gaagtgttaa gtgatgaaca
      421 acgtcgcgct gagtatgatc agatgtggca acatcgcaac gatccgcaat ttaaccgtca
      481 gttccatcat ggcgacggtc agagttttaa tgccgaagat tttgacgata tcttctcgtc
      541 aattttcggt cagcatgccc gccagagccg tcaacgcccc gccgcacgcg gccacgatat
      601 tgaaatcgaa gtggcggtat tcctcgaaga aacgctgact gagcataagc gtaccatcag
      661 ctataacctg ccggtttata acgcctttgg catgatcgaa caggaaatcc cgaaaacgct
      721 gaatgtgaag atcccggcgg gcgtcggcaa tggtcaacgc atccgtctga aaggccaggg
      781 gacgccgggc gaaaacggtg gtccaaatgg cgatttgtgg ctggtgattc atattgcgcc
      841 acatccgctg ttcgatattg tcggccagga tctggaaatt gtggtgccgg ttagcccgtg
      901 ggaagcggcg ctgggtgcta aagtcaccgt tccaacactg aaagaaagca ttttgctgac
      961 tatcccgcca ggcagtcagg ccgggcaacg attgcgcgtt aaaggcaaag gtctggtgag
     1021 caaaaaacag accggcgatc tgtatgcggt actgaaaatc gtgatgccgc cgaaaccgga
     1081 tgaaaacact gccgcgctgt ggcagcaact ggcagacgcc cagtcgtctt ttgatccacg
     1141 taaagattgg gggaaagcat gatggctaat gttacggtga cttttactat taccgaattt
     1201 tgcctgcata ccggcatctc tgaagaggag ttgaatgaaa ttgtcggttt gggggtggtt
     1261 gaaccgcgtg agatccagga aacaacctgg gtatttgacg accatgccgc cattgtggtg
     1321 caacgcgcgg tacgcctgcg tcatgaactg gctctggact ggccggggat cgcggtggcg
     1381 ctgacgttaa tggatgatat tgcacacctg aagcaggaaa accgcctgct acgccagcgg
     1441 ctttcccggt ttgtagctca tccgtgatgt gtgtttgccg gatgtgatga cgcatccggc
     1501 aactgcgcca taatgcctga tgcgacgcta aacgcgtctt ttcaggcgca taaacgacgt
     1561 tatctgtttt ggtggtcgca ctccactaac accagcaata actggcttag tgccgcgtaa
     1621 aaaccaaagc tgtcatactg atggcaacgc gcagcaaact ctggtaacca ttgccacagc
     1681 gccgtcagtg ttttttgccg caaactgtcg attcttcgcg caggaacggt cccctctccc
     1741 agcgaaaaat gcagatgact caacaactcg agatagatcg ccagatggtc tgccggttcg
     1801 ttgaaattgc cgctggtttc catccctgcc tcaactaaca agcgtttaat ctcttgctcg
     1861 tcctgtttgt aggccgatgc atacggcagc gccgcttgtt tgtcggtcat cagaaacagg
     1921 ccgcaaaagt cagcggccag ttccagacgg gcatcgtcac gtactgtcag cgtggcaata
     1981 cagttttcca gctcattcac cgccgcagcc agcggcggtt cgcttttcag caacgaaaac
     2041 cattcagcca tctgcgcact ggcgatttgc gtcagttgtt catcgtccag ttcacgggag
     2101 aacaactgcg ccagccaggc gtaaacacag gcaatctgtt gtgctgtcag cgtggtcatg
     2161 atttcacctg cgacgcggga acatattcgc actgcgccac catctccaca gggccgttaa
     2221 acgccgtcac ctgctccact gctccattgt acttctcaat ttccaccagc gtagtgtgcg
     2281 cactggtcgc ctgcgccagc tgcgaagtac cgatgtcgat ggtcagcacg ttggggttac
     2341 cgtatttgca cagcgcacca ggctcgccgc ctttatctgg atcgtaccat gccccttcgt
     2401 gaattcgtgc cacgccgggt gcatagcggt cagaaactac tgcccctgcc aacacctgac
     2461 cgcgagcgtt aaagacgcgt accacatcac cgttacgaat accgcgcgcg ctggcatcct
     2521 gcgggttaat gaacactggc tctttacccg ctaccgtata ttgctgacgc agagtttccg
     2581 actcacataa ctgcgagtga agtcggaaat ccggatgcac agattgcaga tgcaacggat
     2641 acttctgcga gccgggccca ccgtgggagc gttcgatttt ctcaaaccac atcggatgcc
     2701 cctgacaatc gtcgtagttc atatcggcga tggttttcga gtagatctca atcaggccac
     2761 tcggcgtgcc cagcggttcg agatccggat cttcgcggaa tgcctggtgg cgaacaaaca
     2821 tctgcggatg gtcaaactcg acgtactctt tgttattcca gaagtcatca aacgctggca
     2881 gatgaacgcc gcgtcctttg ccttgttgta caccttcctg ccagatgcgt ttcagccagc
     2941 ccatttcgtc cagcccttcg gtaaaggctt cttcgcgatt aaagcgacgg cacagctcgc
     3001 ggaaaatatc aaagtcgttg cgcgcctcga actgcggcgg caccacctgt ttcatggcga
     3061 taacgccacg gttggagtgg ttgccgtact ggtcgagatc gttacgctca aactgcgtgg
     3121 tcgcaggcag cacgatatcg gcaaagcggc aggttgaggt ccactggtta tctatggcga
     3181 taaccgtttc cagcttgcgc cagccttcaa taatgcggtt gatctgctga tggcgatgga
     3241 atgggttagt tccggcaaaa atacacattt tcagcggcgg cagttttacc gatttaccgt
     3301 tccagttgat cactttcccc ggttcgagga tcgcatcgat aaaacgggca atcggaatgg
     3361 tgctgctgta gcctttgtaa tcactgttgt cgtgaacagg cggaatcgac gtagagccgg
     3421 agaaaccact cagaataacg cctttacgcc ccggcgtgcc tgcgccgtta tagtgccagc
     3481 caaaaccaaa gccaccacct ggcaggccaa tttgccccag catcgccgcc agaaccacaa
     3541 tcatccacgc ccactgttca ccgtgctgca tacgctgcac gcaccagcca gcaataattt
     3601 gcgttctgtt cgccgccatc tgccgcgcca gcccacgaat ggtttcggca tcaatgccgg
     3661 tcagtttttc agcccatgcg gcatctttcg gctgaccgtc tttctcaccc agcaggtacg
     3721 gcaggaactg ctcaaaaccc acacagtagt tagcgaggaa gtttttgtcg tacaggtttt
     3781 cactgtacag cgtatacgcc agcgccagtt gcagcggcac atcagtttgc gggttaaccg
     3841 caatgtgctt cacatgctcg cgccccagat actcatgggt ggatgtgaca accggatcga
     3901 tgctgatgac ctcaatttca ccggcggcga ctttcgcttt tagctgcgcg taatattcat
     3961 aaacatcgtg atccgggcac caccagttcg cttgctggtt tttcagcaaa tcagagcccc
     4021 acagcacaat ggttttgctg ttctgcaata ccagcggcca ggaggtttgc tgttcataca
     4081 cttccattga gccaaccacg cgcggcagga tcacctgcgc agcaccggta gagtaatctc
     4141 cgcccgtacc aacgctatta ccatgcaagg caatagcttt cgccagcatc cccgaagcgt
     4201 tatggaacat ccccgtcgat tgccaaccac tggcggtcag caaggcactc ggcccgtgag
     4261 ttttctgcac gcgttccagt tcttcataga acatgtcgag ggcttcatcc cagctcacgc
     4321 gcacaaaacg gttatcaccg cgctgggagg tatcgctgag atggcgctta cgcagccagt
     4381 ccacgcgtac catcggataa cgaatacgcg ccgcgttgtg tacgtgatcc ggcaatccgg
     4441 caatcatttt cgacggatat ttatccagtt cgaacggttt tgccgccaca aagcgaccat
     4501 ccttcaccgt cgcgcggata gccccccagt gcgacccggt aagaatgccc tctttcgaga
     4561 tgacagcgtc agtcgccgct tgcgccgcag tcgcacggcg cggcgttaac aatgacggcc
     4621 ccagcatccc ggcgacggtt aagccgccga gttgtgccag aaaacgccga cgtgatgcct
     4681 gaaagagatc gttattgttc attatttttc ttccttctta tcgccgtgag ccttacctgc
     4741 ggtgtcagac gcattcattt gcagatattt caacaaggtg cgttcttcac gtttatcgag
     4801 actggtaaag ccaatcatgc cgttgagcgt gccgatccaa ccgttagcgt caaagtgggc
     4861 gatttccggt gcgccgtggc actggttaca ggtgccgttg tacaacgaat ccgcataagc
     4921 ccagatcggt ttgatatcgt tcaccatgtc gcctttcttc atccacgcag tggcctgcaa
     4981 cttgctccac tcggtattgg tgtcggcaac ggtggttttc tccagcgttt ttacctgctg
     5041 ctgcacatca ccacgaatcg aggcaacaaa gatgcgttta cctgggaatt gggtgagtac
     5101 acgctgacgt ccggcgcttt ccgtccagcc ggtaatttca atttgcagcc agtcgccgtc
     5161 acgtttaagg actttcactt ccgaagcagg cagcagagaa ccagaggctt ctttatcgcc
     5221 tttcgccgca taaattggct taatatcaat agagtacagc gtgtcaccac tgtcattagc
     5281 actggcgcgc agctcatcga actgcttacg gaagccgcta ctcatatccg gtaactggtg
     5341 ggcaatacct ttatgacagt cgatgcagga ttgattatct ttcgctgcca ccttcatctg
     5401 acgtgccgct tcaggatgct gcttcgcatg atccatcgca tcgtagttat ggcaggagcg
     5461 gcaggttgcc gagttgtttt ctttcattcg cgcccattca cgctcggcaa gttccgcgcg
     5521 tttggcttcg aatttttcag gtgtatcaat ggagtgagca ataaaggtct ggtagatatc
     5581 attgctcgct tccagtttgc gcttcaccat gcctggaata tccggcggga tatgacagtc
     5641 atggcattca gctcgcacgc cggaggcgtt ctggaaatgc accgactgtt tatattcttc
     5701 atacaccggt tgcatactgt ggcaactgac acaaaattcg gttgtgctgg tgactttgat
     5761 cccaacgtgt ggcaatacaa tcagcgcaat gccaatcaca atcccaattg cgaccagcgc
     5821 cagtaccgac caacgagcac tgggtcggcg tagcgcgttc cagagtttcc gcataatagc
     5881 ccctgtaaaa ttatggttta gtgaagcgat cttaatgagc aaatatgaac agcggcactg
     5941 gtcaggatga acggcttacg gcagaatatg aacagatatg aacagaatga gtaaaaccct
     6001 ctgatgccac atcacattgt tattgttgaa gatgagccgg ttacccaggc gcgattacaa
     6061 tcttacttca ctcaggaggg gtataccgtt tccgttacag cgagcggtgc cgggctgcgg
     6121 gaaattatgc agaatcagcc ggtagattta attctgctgg atatcaactt acccgatgaa
     6181 aatggcctga tgttaacccg cgccctgcga gaacgctcaa cggtggggat tattctggtt
     6241 accggacgca gcgatcggat tgaccgtatt gttgggctgg aaatgggcgc agacgattac
     6301 gtcaccaaac cgctggaact gcgcgaactg gtagtacggg tgaaaaatct gctctggcga
     6361 atcgacctcg cgcgacaagc tcaaccgcac actcaggaca actgctatcg ctttgccggt
     6421 tattgcctga atgtgtcgcg ccatacgctg gagcgggatg gcgagccgat taaactgacc
     6481 cgcgcagagt atgaaatgtt ggtggcattt gtgacgaatc cgggcgaaat tctcagccgt
     6541 gaacgtctgc tacgtatgct ttctgcgcgt cgggtggaaa accctgacct gcgcaccgtc
     6601 gatgtgttaa ttcgtcgttt acgtcataaa ctcagcgcgg atttactggt gacgcaacat
     6661 ggtgaaggtt atttcttagc cgctgatgtg tgctgataaa aatagaccgg acgaaatccc
     6721 cctggtgaca gcgagcggcg aatatgttca cggtcggcat ttttcggcgt cagaactaaa
     6781 atcggtgggc tgacattatc agacaccgat tgcccctgta attgcctgat ggcctgctca
     6841 accgccagtt ccccctgcca gaccatttga tcgctggcag ccataatcac tcttccccgc
     6901 ttcagcccgc gatacacctg atgtgaaaga taaaacgaca ccacggtaag cggcgttttc
     6961 aggttacgcc cttcacccat tgccgcctct gccgcaatgg ccgttccggc aacgacgtcg
     7021 atttctgggt ggcgttccag catctcctgc aacaggttac gctggatttc aatatcgtta
     7081 tcaccaagcg caatatcaac aatacgcacc gggcttccgg caatggctgc gcgaaaaccc
     7141 tcgaccatct ctttactgcc cccggcatta tcgggtccgg gcatcaacag cacgttcagt
     7201 ggtttaccgt gcgcccattg caccaaatat cgcccaggtt gatagcccat ctgaaaccag
     7261 ggtacaccaa cgcggctttt caccagggga tcatcaagag catttaccag ttcgatcacc
     7321 ggcagatttg ctacctgctt ttgcaggtcg ggaaatgacg tagtgctact accgagcaaa
     7381 atggcctctg cgccccactg tttacactgg tcgatttgtg cttgctgggt agccaactgg
     7441 ctgtagccgc ctgcctccag cacttttaaa tccacaccat agcggcgagc tgcctcctgc
     7501 ataccatagt tcaacgataa ccagtatgaa tctttcaggc tgggataaag cgcgcacagt
     7561 ttccatgcgc gtttggcttt aagcggcgta gaggcttgca ccgtgtaatg ctgcgcatca
     7621 tgccagcgca acaggttatc agccgaaaat gccgataaca tgaaaaggga aagaagtaaa
     7681 aatagcagta cgcgcatgat agcctcatca ataataaggc tttatgctag atgcattccg
     7741 ctttgcgact caaccttttt cacctaaagg atgacaaata cacacaaata ttttaaaaat
     7801 catattgtta catgaattca tgattaaata acagactgca aaatacgata aaacactaca
     7861 tctagagtca ctattaatca atgagttaga tgtggttagt gacctacaac agagcattag
     7921 cgcaaggtga ttttttgtct tcttgcgcta attttttgtc ataaaaacat gtggcagact
     7981 ttgtatatct ctgctttgcc acgccacacc atcatgcatt atggctatgc ctaaacagaa
     8041 tcaataagaa tagaacataa aaaatgacaa ctctactaga caattaacta caaaaatgat
     8101 agcaattagt ttatataata taagacttcc tatcaaaagt tcatccatat gaagacaagg
     8161 aatttattat gccaatcata aagaactgct tattatcaat tagtaacata ttacgcaacg
     8221 aaaaaacatc atattctcta ataaaaacag agcaaacagg aaaactgtta aacagaaaaa
     8281 taaccaccac tcccacacca gcgaaactat tatcatatcg aaatgcagat ttaataaaag
     8341 aaaactacat tacagaaaaa gttttatcta ttttcaatat aaaacgggat ttcgttgctg
     8401 taagaatcca aagcaatcag tttactgatt taaaaaacaa gacaattcag ggtcataaag
     8461 acactgtcgc aaaagtcatg gactggtata acccacaaaa aaacgcattt ggtatcatga
     8521 tgggcacacc aagacgttct gctgatatag caaaggaaga atccagaaac gctctaaatt
     8581 tcatgataat ggaaaaggat acatttaatg aaaagatact taacagcaat gataatttac
     8641 aaaaaaaata cggcacaaca gaaaatagta gttgggtatc ggcttctgtt ggttctcttc
     8701 tggacaaagg agccaaagta tatccagata tatcatgctc tctgagactg ggagaaccat
     8761 tcattattac acttccagaa acagtcaggc tggatgttga tatccaccca ctcaaaaaat
     8821 gaacctgaac gttttcatgc aggcaaatgc gtccacatca aagcttcaac caggtgctct
     8881 cctcctgtca caggataaat ttgtctgtaa aaatcaaact ctcccaactc attaccaata
     8941 ccaatcagtt gaatgctgtt gccatctaca ataaattttt ccgctgacgg aaccaaaatt
     9001 aaggcttccg gatgttcatt aggaaaggtg ttagcaagag tacgctgaat atccctctcc
     9061 agcaaaatct cctgagtatt agcgtaaagc gctggcgtaa actcatcgac gaaatcgtta
     9121 ctatcccttg ggcgcatatg tatcattgaa tacgactgtt ctccagattc tgataatcca
     9181 tggcgaacaa taagaacact acagccgctt tgctgattgg ttagccataa cggtgaactt
     9241 tgctggatag tttgagaaat atcatttcgc cggaggctta tacactgatt actcaagctt
     9301 acaactgcag gtgcatcaaa gcggtccgga ctatcagtat cacgcatacc tgtaaacaga
     9361 atataatcac taccagccat atcatcttcc gtttctttta gtcttaacaa gccatcctct
     9421 cttgtaatat taactgtaat taaagatgtc tcatcatgat gggcatagcg ctgtggatta
     9481 agaattaaat gctcttccag aaattttaca gggttactac gtatttcatt cattatacac
     9541 cgaagctctg aacgatgcgg caccagaaag tgcccggtat tttgtgaaat aatactactt
     9601 accttgattg ttttctctcc agctcatgaa aaataactcg cgtattatat tgttttacca
     9661 accacaggcc ccgaatgcct gtggtgtttt tatataaatt atcggaagtt aatattgttt
     9721 catttgaaac atcttaacta tcttttataa taaagcttcc ccttgcaggg tcatatttac
     9781 actcttctgg tttaacaatc attgatgggg ttattggagc tcgtgtcaga gggtgaactg
     9841 aaccgtcatt aaccacgcga gaaagtgcat caacatcaaa taaagtgcat actgctgaac
     9901 tatctgaatt tttgacaaat accccttctt ctggtgtttc cagtataatt gggcatcgag
     9961 ttgcatcaga gggacactgg aggctctctg tattaacaga aaacttacat tgatatattt
    10021 tatcctgagg agtcacttgc gattgtgttt cacctgcatc tgatcctgaa agaatactta
    10081 acattctctc gccaagagct tgaggcccat tattgaaccc taaaagcaat aattcagtaa
    10141 ataatccccc agtgccatcg ctggccagaa agcgcccatc tgttgggcta tattcaattc
    10201 tgacagtttc accacccatt gtaaattctc cacgcccagt ggctgcaact tgagcacgaa
    10261 gaacctccat tccaagatgg aacgaagatg aattaatatc tgaggttaat ggcataataa
    10321 agctccctgt tttaatttga actccagact taaaggtcca caacaaatgt catccacaaa
    10381 tttttccctc gtcccatcag cacaccgaat ttgcagcggc ctcaccacgc actgtatcgg
    10441 ctttttatcc gcacccagta tcaccacctg cgtgattacc ctgtcctgct ccggaataat
    10501 gccctattcg cttgttctca gaaattgcca gcaaccttac actacttctt ttgagcccat
    10561 ttttcgccag ccctgaatac aggaaagtgt taactgtttt tatgtttacc ctcatctcac
    10621 tggcgagatg ggacggacgc aactgacggt agatatatcc aaacatcacc accatctctg
    10681 acatcgtcag tgcccgtgct tttttgttcc aggtccacaa tctgttacgc acagcattat
    10741 gtatatcatg tccccctttc aacgcttcca gatccagctg aataaaacgg cgtccctgaa
    10801 acatgctgcg cattaaatgg cccaactgac tatcacagat tattaaccag gattctttac
    10861 tatccagaat aaccctgcga ttttccggca taaaaaattc ttgagccaga attacacggc
    10921 atcctttcag taacaaacgc aaaccatatt ccagataata attacgcgtc gcaagaatga
    10981 gcttcatcgc cctacttccc tgaactacag tagtgttcca tgcctcggtc gcaatcagaa
    11041 tttatgattc cccctccagg gaacaagatg gacactcccc tgctccttaa aggcatcctt
    11101 cactccatcg tattaacatg tttattactt cacactctaa taacaaaca
//
LOCUS       DAZFXT010000145        11108 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.145, whole genome shotgun sequence.
ACCESSION   DAZFXT010000145 DAZFXT010000000
VERSION     DAZFXT010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.145"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(188..907)
                     /gene="dcuR"
                     /locus_tag="ACHKW0_004128"
     CDS             complement(188..907)
                     /gene="dcuR"
                     /locus_tag="ACHKW0_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HFO7476870.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(904..2535)
                     /locus_tag="ACHKW0_004129"
     CDS             complement(904..2535)
                     /locus_tag="ACHKW0_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HFO7476871.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            2716..2946
                     /gene="yjdI"
                     /locus_tag="ACHKW0_004130"
     CDS             2716..2946
                     /gene="yjdI"
                     /locus_tag="ACHKW0_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HFO7476872.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            2958..3230
                     /locus_tag="ACHKW0_004131"
     CDS             2958..3230
                     /locus_tag="ACHKW0_004131"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7476873.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            3457..3753
                     /gene="ghoS"
                     /locus_tag="ACHKW0_004132"
     CDS             3457..3753
                     /gene="ghoS"
                     /locus_tag="ACHKW0_004132"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HFO7476874.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            3781..3954
                     /gene="ghoT"
                     /locus_tag="ACHKW0_004133"
     CDS             3781..3954
                     /gene="ghoT"
                     /locus_tag="ACHKW0_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HFO7476875.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(4073..5590)
                     /gene="lysS"
                     /locus_tag="ACHKW0_004134"
     CDS             complement(4073..5590)
                     /gene="lysS"
                     /locus_tag="ACHKW0_004134"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HFO7476876.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(5827..7284)
                     /gene="dtpC"
                     /locus_tag="ACHKW0_004135"
     CDS             complement(5827..7284)
                     /gene="dtpC"
                     /locus_tag="ACHKW0_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HFO7476877.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMVKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(7343..9490)
                     /gene="cadA"
                     /locus_tag="ACHKW0_004136"
     CDS             complement(7343..9490)
                     /gene="cadA"
                     /locus_tag="ACHKW0_004136"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HFO7476878.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(9570..10904)
                     /gene="cadB"
                     /locus_tag="ACHKW0_004137"
     CDS             complement(9570..10904)
                     /gene="cadB"
                     /locus_tag="ACHKW0_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043872 - lysine:cadaverine antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HFO7476879.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
BASE COUNT         3019 a         2616 c         2626 g         2847 t
ORIGIN      
        1 taattaactt ttgttatcgt tttgaaatta aaaacaccgt tcacctgaag agatattaat
       61 ttttagcgat gatggaggga taattatatt tgatctggca caagttttac tgatgaagga
      121 tgtaacttgt gccaggggta ttttgcatta cggtaataat tattacttac agataaccag
      181 cgatccgtta ttggcaatat tgtttcagta gtgagtagtg ttctgcctga atacggtaac
      241 ggtaaaccgg acgccccgtg acgccataat ggatactggt gaacaagatg tggcagttga
      301 ccagccagat gaggtattta cggcaggaaa cacgcgagat gttaacctcg ttagccagct
      361 cgtcggttga aaactcatag tcctgatgcg cgtcaatcca ctggcacagc gtgcgtaacg
      421 tctgcggcgt taagcctttt ggcaagcgac gaggatcctg ttcgttggag ctgctgccgt
      481 ggattagctg atcaagctcg gcctggtcat aatactgatg tttttccagc gccattttct
      541 tttgccgcca gccggtgagc gcctcttcaa agcgggaagc ctggaagggt ttgatcaggt
      601 aatccacgac accgtaatgc agcgaatctt taatggttgc cgcatcggct gcggaggaga
      661 tgacaatcac atcacttttg caacgcgcgt tatgcaggac aggcagtaaa tcgagcccgt
      721 tctctttttg catatagata tcgagcaata tcaggtcgat aggcgtatcg ctattgaaga
      781 taatctcttt ggctttctcc agcgtcgagg ctgttccaca gcattgaaag cctgggattt
      841 gtgctacgta tcggcgattc agctccgcga ccattgcgtc gtcatcgata attaatacat
      901 tgatcatctg ttcgacctct ccccgtccca gggtatctgg acaaaaaatt gtgtgaaaat
      961 cccgggttcc gattccacgg cgatgctgcc gccgagattt tctacctgtt gtttgacaag
     1021 tgctaaaccg acgcctcgct cgcttccttt tgtcgagaca cctttgtcaa aaatgtgatc
     1081 gattttgtcg ggtgcgatcc ccggtccatc atcgttaact tcgcagtgca gccagccgtg
     1141 acggtagtgc aatgttacgc taatttcgcc accgggttcc ggccctaatg cttccagcgc
     1201 gttttctatc agatttccca atgtggtaat cagcgtcgcg acctggtcct cactgccgct
     1261 gtctggcagc tggctttcac tgtttaaaat cagcgtatgg cctaaatcgg tcgcgcggtt
     1321 aatcttgctg attaaaaaac cagcgataac cggagatttg atcttaccca gcagagagcc
     1381 aatctcttcc tgatagttat tggctgtttt gagaatgtaa tcttccaact gcttataact
     1441 cttcagatgc aataatccga gaatcacatg caatttattc ataaattcgt gggatcgttc
     1501 acgaagtgcg tcagcatagt tgaccagacc atcgagtcgc tgcatcagtt tacgtacttc
     1561 agttttgtcc ctgaaggttg aaatggcacc gatgataacg ccattactgc gcaccggaac
     1621 ggtgttgatc agtaatagcc gatctttaat cgtaatctct tcgtcgcggc gcggcgtacc
     1681 gtcgcgtaac acttccgaga catctaccac ctgtgaccat gagtggctga gcgtcgacag
     1741 tttctcatcg tcctgcgact tacggtaatt cagcaattct tgtgcggcat cgttgatcag
     1801 cgtgacctcg ccgcgatcgt ccacggcaac gacgccttct ttgatagact gcaacatggc
     1861 ctggcgctgc tcaaacagcg tggagatttc gtagggttcc aggccgaaaa ggattttttt
     1921 cagtacctta accagaatgc aggtgccaat cagtccgacc agcatgccaa ataataccga
     1981 ccagataatg ctccagcgac tgtcattgat ttgttgggtc acgcggctta actcaaggcc
     2041 gatcgccacc acgccaattt gtttatgatt ttcatcgtag atgggggtaa atacgcgtaa
     2101 agcctgcgcc agaaaaccgc gattgatagc gacattttct ttgccattca gcgctttaag
     2161 gatgtcatca cctttaaatg gctgaccaat acgctgggct tcaggatgcg agtagcgaag
     2221 actttgcata tcggtaacga caataaacag cagatcgttg cgtttgcgca cggcttccgc
     2281 gatggcctgg attccgctct cctgcggttt tttctgcaag ccctgacgga tttccggcga
     2341 gtcggcgagg gtacgcgcca ctgccagtgc cttgttggct agcccgtctc gcgtcatatc
     2401 actgatttgc gagaagtaaa tcagatgcac caccaatagc accgagaaca gtaccgcact
     2461 gaccattaag atcactgtgg tactcaattt catcggacgt ttgcgtaaca tgcggtaggg
     2521 caatgaatgt ctcatcagct tccttgtgtg acaaatttct taagcattat ctctgatgag
     2581 gcgggtaatt caaagggagt aagaatgatt ggctatatag gggaagagac tctggcaacg
     2641 gaaactgcca gtgctgtatg aagattccag ggctatgctt atagcgataa tcatactgat
     2701 gagagaggga aagtcatgga tcaggcgcta ctggacgagg gttatcgctg ttataccggc
     2761 gaaaagatcg atgtctattt caacactgcg atatgtcagc attctggcaa ttgcgtacgt
     2821 ggcaacggca agttatttaa tctcaaacga aagccgtgga tcatgccgga tgaagtcgac
     2881 gtcgtcactg tggttaaagt gattgatacg tgcccgagcg gcgcgctgaa ataccgtcat
     2941 aaataagcga gggtaaaatg gaaatacgcg aaggccacaa taaattttac attaatgacg
     3001 aacaaggcaa gcaaatcgct gaaattgtct ttgtgccgac cggagagaat ttagcgatta
     3061 tcgaacatac cgatgtcgat gaaagcctga aagggcaagg gattggtaaa cagctggttg
     3121 cgaaagtcgt ggaaaaaatg cgtcgggaaa aacgaaaaat tatcccatta tgtccatttg
     3181 cgaaacacga atttgataaa acgcgggagt atgatgatat tcgcagttga tgggagagta
     3241 cagagtcacg atatttttct tgctctccgc ggagtgatgc aggagagcat ctgaagggta
     3301 gggggatgca caaagaatgg gcagagagcg cgtttttttg tcccaagtca tcccctttac
     3361 tgagcaaaaa aaagaatatc tcctatatga gaatcatcaa tcggggttaa taagttttgc
     3421 gtccccagag cattcaatat tgataggagt catattatgg aaggtaaaaa caagttcaat
     3481 acttatgttg tttcttttga ttatccatca tcttattcct cagtgttctt aaggttaaga
     3541 tcattgatgt atgatatgaa tttctcctct atcgtggctg atgaatatgg gataccacga
     3601 caattgaatg aaaactcctt cgcaataacg acatcgttag ccgcaagtga aatcgaagat
     3661 ttaatcaggc ttaaatgctt agacttaccg gatattgatt ttgacctcaa cattatgaca
     3721 gttgatgact atttccgtca gttttacaag tagccaggaa ggataagaag aacataaaaa
     3781 atggcactat tctctaaaat attaattttt tatgtgattg gtgtgaacat atcctttgtc
     3841 attatctggt ttatctcaca tgagaaaaca catattcgtt tacttagtgc attcctggtc
     3901 ggaataacct ggccaatgag tctgcctgtg gcattacttt tttctctctt ttaggagctg
     3961 tagttgagtg gacccgctgg ttatcgtgaa acgaagcgtc tctcctcaaa actggatttg
     4021 cggaacgacg aacgaagcag tcgcctgact gcttcgttca ttaaagtgaa atttatttct
     4081 gtgggcgcat cgccgggaag agaataacgt cgcgaatagt atgactgttt gtaaacagca
     4141 taatcattcg gtcgatacca atacccagac cagcggttgg cggcagacca tattccagcg
     4201 cagtcacgta atcttcgtca tagaacatgg cttcgtcgtc acctgcagct ttagcgttaa
     4261 cctgttcctg gaaacgttca gcctgatctt ctgcgtcgtt taattcgcta aaaccattac
     4321 cgatttcacg accaccgatg aagaattcaa aacggtcggt gatttccggg ttaacatcat
     4381 tacggcgcgc cagcggtgac acttctgccg gatattccgt aataaaggtt ggctgaatca
     4441 gatgtgcttc tgccacttca tcaaagatct ccgtgacaat acgtcccagc ccccagcttt
     4501 tctctaccgt aattccgata gattcagcta atgctttagc agcatcaaaa ttatccaggt
     4561 cggccatatc ggtttctgga cgatattttt tgattgcttc gcgcatggtg agtttttcaa
     4621 acggtttgcc gaaatcaaac acatgctcgc cataagtgac tttagtggta cccagaacct
     4681 cttgtgccag ggtgcggaac agggactctg tcagttcaat caaatcgtgg taatccgcat
     4741 acgccatgta gagttccatc attgtgaact caggattatg gcgaacagaa ataccttcat
     4801 tacggaagtt acggttgatt tcgaataccc gttcaaaacc gcctacaacc agacgtttca
     4861 gatacagctc cggcgcgata cgcaggtaca tatctaaatc cagagcgtta tgatgggtaa
     4921 taaacgggcg agcagatgcc ccacctggaa ttacctgcat catcggggtt tctacttcca
     4981 taaagccgcg cgcgaccatg aactggcgga tagcggccag aatttttgaa cggacaacaa
     5041 acgtttgacg ggatttatcg ttagcgatga ggtccagata acgttgacga taacggactt
     5101 cctgatcctg cagaccatgg aatttatctg gtaaaggacg tagtgctttt gtcagcaggc
     5161 gtaactcagt acagtgaatg gaaagctcac cggtttgcgt cttaaacagc gtaccgcggg
     5221 caccgataat gtcacccaga tcccattttt taaactgatc gttataaaca ccttctggca
     5281 ggctatctct tgcaacgtac agttgaatac ggccaccgac atcctgcaac gttacaaagg
     5341 aggctttccc catgatacga cgggtcatca ttcggccagc aaccgagact tcaatgttta
     5401 aggattccag ttcctggtta tccttcgcat caaactcttc gtgcagctgg tcagaggtat
     5461 ggtcgcggcg aaaatcattg ggaaacgcca caccttgctg acgtagtgcc gccagttttt
     5521 cgcggcgatt tctcagttca tcgttaaaat caatagcctc attggctccc cgtgtttctt
     5581 gttcagacat tttggttcct ctaaatccag ctttcaattt agcgtagata aagagacaga
     5641 tcggtacact gtattatctg cctcgactat taataactca gtttatctta tcgaaactaa
     5701 cgcttacctc aagttgatat cactaataaa taaccatcaa aatggtttaa atgacttatt
     5761 aatctagatt tgctagtgtt ataaactgaa agttaattta ttcataacca attgttttta
     5821 cgacagttaa tcgttgctct cctgtatcat attcgtcggc gtgctaaaca gaaaacgggt
     5881 agcaaaggcc aatacgacga tgatcgcgac acaagccaac gtccactctc ccatctgaga
     5941 aaagaatcgc tggtaagctg caattgccat accgctaatt tgcgactctg tcgtctgctg
     6001 tgccacgacg cctgccagcc agttggcgac cgcgcctgtt gccagcatat aaataccggt
     6061 taatacgcca gacattttca gacgcgtgat ttgcgcaatc gccaccgggt caataaagag
     6121 ttcggcaaag cccattagcg ccaacccgga tatcatcacg cccattgacg cttgaccgtc
     6181 agccgctgca tgtcgggcat caaatgccaa caacataaag ccacaagcca tcagcagtaa
     6241 gccaaaggca aacttcagcc agacgcgcaa tgttgagttg ccgcggcttt ctggcgacgc
     6301 cagccaggcc agtacaaccc cagcgagcat caccgcaatt gcattcaccg actggaatag
     6361 tgctgtaggt acttcaatat tgaatgcctg acgattcaca aagcgatcga taaacaagct
     6421 gatggtactg ccgccctgtt gtgccagtac ccagaacaat gtcccgacaa acatcaacaa
     6481 tacaatttgc caaagagcac ggcggtgttc ggggaatttg accatcatgc gggcaatgat
     6541 ttgtgcggca atgaggcaaa cgatcgccag caaatatccg gaccagtcgt tctccagcag
     6601 cagggtaaaa aatactgggg ctaaacagag catcaccact aaccagctcc ataccggtaa
     6661 ggcaaatttg acgctagtga gcgctttttt atccatacta cgcgtggact ggaaatgacg
     6721 atgcccgctt aagaaaatca acaaaccgat aaacatgccg ccacccgcaa gggcaaagcc
     6781 aacatgccat ccataccact gagcagccag gccgcaggcg attggggcgg cgatagaacc
     6841 gatattgccc gcagcataca gcagcgaaaa accgccatca cgtcgatgat cgttctcgtc
     6901 gtaaagctcg ccaagcaaac agctgatgtt tgatttgaat aaaccgtagc cacaaataat
     6961 gattgccagc gccagataca ggctaaaggt tgaatttgta tcaatgccca gcaccacatg
     7021 gccaagggtc attaacagcg cgccggcaat cactgcagtg cggttgccga gcaggcggtc
     7081 ggcaagccag ccgccgagaa taggggtaac gtaaaccaga gaagcatatg cgctgaacag
     7141 gctgatggca tggttatcat caaaaccaag ctgatgggtg agatagagaa tgagtaaggc
     7201 acgcatgccg taaaaactga agtactccca gatttggatc gccacgatat agtatatcgc
     7261 gcgcggctgt gagggtgttt tcatgtgttc tccttatgag caaaaaaggg aagtggcaag
     7321 ccacttccct tgtacgagct aattattttt tgctttcttc tttcaatacc ttaacggtat
     7381 agcggccatc agcctgacgg tatgcaccgt gaatatcggt ttcaaagccc ggatagtgag
     7441 cgccgatttc acacagcatc tgcaggaact ccagaaccgg acggctttct tcggtgatca
     7501 tttcacccgg cattaccaga ggaactcccg gcggatatgg aaggatcata ttggcgttaa
     7561 tacgacctac catttcgtcg aggtaaactt cttcggtcat accgtgcagc tctttctgga
     7621 acgcagcata cggagtcatt accatcgtcg gcagcacttc aaatgcgcga tacatcagat
     7681 ccggcagatt gtggtgaaca atcagtttgt ggatattctg agccagttcc tgaatacgca
     7741 tgttttcata gaattcagga tcttcacgat acagagacgg cagcatgttt ttcacacgca
     7801 ggttcaggtc gaacgcacgt ttgaagtcag tcagagcacg cagcaggctc agtgctttgg
     7861 tcttatcgat accgatgctg aacaggaaca gcaggttata cggaccggtt ttctcaacaa
     7921 cgatgccatg ttcgtcgagg tatttcgcca cgatgctggc cggaatacca aagtcgctca
     7981 tggtgccgtc tttttccatc cccggagtca gcagggtgac tttgatcggg tcaagataca
     8041 tgtgctcgtt atcgatgttt ttgaagccgt gccaggtgct gtcagaacgc agcggccagc
     8101 attcagtggt atcgatatga tccggctgcc atacatcaaa gaaccagcca tcagattccg
     8161 ttctcagacg tttgatctct ttacggaatt tgatcgcacg ttcaatggaa ccgttgatca
     8221 gacgcttacc agcattgcct ttcatcatcg ccgctgcggt ttcagtggac gccacgatac
     8281 cgtagtgcgg agaagtggtg gtgtgcatca tgtaggcttc gttaaaggtt tcttcgttta
     8341 cgtcaccttt aacgtggatc atggaagcct gagagaacgc cgccagcagt ttgtgagtgg
     8401 actgggtttc gtaaatcact ttcccttcta cacggccacc gctcataccg catttacctt
     8461 cgtaaatcgg tgagaagttg gtgtaaggca cccacgcgga gtcaaagtgg atggatttca
     8521 catccagtgt tttcttgata aagtcggtgt tgtacagcag accatcatag gtagagttgg
     8581 taattacagc atgtaccggc caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg
     8641 tagcgtgctg gaattcactc tgtgggatac caccaagaat accgtaagcg ttacgggtcg
     8701 ggcggaaata gattggcgta acatcgctca tcatcatcag gtgggtcagc gatttgtggc
     8761 agttacggtc aatcagaatg gtgctgcctg ctggagcaga gtacatacca acaattttgt
     8821 tcgcagtgga agtaccgttg gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga
     8881 tatactgttc tgcttctttg tgtggaccac tgtgatccag cagagaaccc agttcagata
     8941 ctgaaatgga aatatcagat ttcatggtgt tcggaccaaa gaaatcatag aacaggctac
     9001 ctaccgggct tttctggaat gcagtaccgc ccatgtgacc aggagtacag aaagtatatt
     9061 taccttcacg aacatattta aacagtgctt tagtcagcgg aggcagaata gtgttgatat
     9121 attcgtcagt ggtctgcttg atcttattag caatatcttc agcagcaccc agcgcatatt
     9181 caaagaagct aatctgtaaa cgcaggtcat tcaggcttac atcgagagtg gaatacgtat
     9241 tagcgaacgc gtacaacggc aggttctcgt tcattttgct aatttcttcg cacagctcga
     9301 gattatattt atcccagtca aaaataacgc cgcacagacg cgcattgttt tcgatcagtt
     9361 ttaataagtc gtcacggtcg ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc
     9421 gatgaagttc acggatgggt tcttctttaa aataaacccc catgtgattc aatattgcaa
     9481 taacgttcat agtcatatct ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg
     9541 aaaggaggag cctcggaaaa tacttttaat taatgtgcgt tagacgcggt gtggttatcc
     9601 attgagtggc tctggcgctc gtgcattttg cgagcgtaga acatcaggat aatcaggctg
     9661 acgatgaagg tacctgccag ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac
     9721 acacaaccca gtacagagca gatcaggctg acaaagttgc ggatgttaac gccttcaaaa
     9781 cgaatcaggt caacgcaaga gtagaagtac ggcagcatag tcagcagtac tgcgataccg
     9841 gtcagttcac cgaacaggtc agatgcttta ccaccggcag agttcatcag agtgatgagg
     9901 atcatcaggg cagtcatttt cactgcagcc agcagcagac cttttttcgg aataccgttg
     9961 ctgtcgactt caccataaac tttcgggaag ttaccgtcgt tagcggcacg tacacctgcc
    10021 tggcctacca acatcatcca ggagcccaga gaagtcaggc acgcaaaggc ggtgaatgca
    10081 gaaaccagcg gcgcagccca gttaccgagg atggttgaag cactgattgc aaacggagca
    10141 ccggaagctg ccattacaga agatggatac ataccggaaa gcacctgagt cgcagcgatg
    10201 taaacaatac ctgctaaacc agtacccagc atggttgcca gcggaacggt acgtttcggg
    10261 tttttaacca taccagtact tactgctgcg gattcaacac ccacgaaggc ccacaggcag
    10321 agcagaatac ttttaatgat cgcatgacca tcagtggtat ccgcagtatt ccagttagct
    10381 gcataagttg ccgcatcaaa ccaatgccag ccaacaatag cggtcattac taccggaata
    10441 agaaccagca ccagaccaat agtggttaaa cggcttaccc aagtaccgcc gagcatattt
    10501 acaaaggtaa atacccagac gatagcaata caggcgatac ccgccggaac aggatcattt
    10561 aatactggga agaaggtgga aagataagat accgcggtaa taccaatcgc caggttacca
    10621 atccagttag catggtaata aagaacacct gtctgaaaac caaatgcagg ggaaatttct
    10681 ccggcataag caattgggcc accttgttgc gggttttttg ttgccagtcg ggcatataca
    10741 tacgccagcg acattgcacc aataatagag ataatccaac cccagatagc aataccaccg
    10801 atacttgcta ggttcgcagg taataatgca ataccgctcc ccatcatatt accggcaaca
    10861 acaccggtac aggcaaatag cccgatcttc ttggcagaac tcatgctctt ctcctaattt
    10921 catttttgaa tttggagtcc gggtcatgat gtataactat ttcctgacca gaccaaactg
    10981 gcgctaagaa tactcacgaa aaaaggatta atcctaaaga ttaggtgaaa taacacaaaa
    11041 gtttctgtaa gtgagaactt gaggtttttt attaacacat caggatcgca agttgatatc
    11101 atgaaaag
//
LOCUS       DAZFXT010000146        11046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.146, whole genome shotgun sequence.
ACCESSION   DAZFXT010000146 DAZFXT010000000
VERSION     DAZFXT010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.146"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            124..840
                     /gene="nanC"
                     /locus_tag="ACHKW0_004138"
     CDS             124..840
                     /gene="nanC"
                     /locus_tag="ACHKW0_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HFO7476880.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            860..1966
                     /gene="nanM"
                     /locus_tag="ACHKW0_004139"
     CDS             860..1966
                     /gene="nanM"
                     /locus_tag="ACHKW0_004139"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HFO7476881.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2031..3011
                     /gene="nanS"
                     /locus_tag="ACHKW0_004140"
     CDS             2031..3011
                     /gene="nanS"
                     /locus_tag="ACHKW0_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HFO7476882.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGVLIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMIEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHAYEVIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSAAARRGIISDRFVEAILQFWGEK"
     gene            complement(3079..3141)
                     /locus_tag="ACHKW0_004141"
                     /pseudo
     CDS             complement(3079..3141)
                     /locus_tag="ACHKW0_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            3223..3366
                     /locus_tag="ACHKW0_004142"
                     /pseudo
     CDS             3223..3366
                     /locus_tag="ACHKW0_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_080685909.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
     gene            complement(4166..4537)
                     /locus_tag="ACHKW0_004143"
     CDS             complement(4166..4537)
                     /locus_tag="ACHKW0_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7476883.1"
                     /translation="MQTISSHPTRATQPCLSPVEIWQRLLTCLLSQHYGLTLNDTPFS
                     NETTILEHIDAGVSLCDAVNFLVEKYELVRIDCNDCSVMEKSPFITSIDILRARKASG
                     LMKRNSHKTVTRVTAGRLQEL"
     gene            complement(4576..4941)
                     /locus_tag="ACHKW0_004144"
     CDS             complement(4576..4941)
                     /locus_tag="ACHKW0_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7476884.1"
                     /translation="MNNHSESGTKPENPACQQWGLKCAITPCFGARLVQEGNRLHFLS
                     DRAGFSGAFAVDVAMRLDQAFPLMMKQLELMLTSGELNPRHPHCVTLYHNGLTCEADT
                     LGSCGYVYIAIYPEQTEPQ"
     gene            complement(4967..5188)
                     /locus_tag="ACHKW0_004145"
     CDS             complement(4967..5188)
                     /locus_tag="ACHKW0_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7476885.1"
                     /translation="MKIISKRQAMALYRQHPGSRLFRFCTGKYKWSGSICHYAGREVQ
                     DIRGVLAVFAERRQDRNGPYVILRSITLN"
     gene            complement(5185..5727)
                     /gene="radC"
                     /locus_tag="ACHKW0_004146"
     CDS             complement(5185..5727)
                     /gene="radC"
                     /locus_tag="ACHKW0_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017694582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HFO7476886.1"
                     /translation="MNTTISPLLSQPELFPVSVMAQHGCLLPTTSGLTPYAQRTIRRA
                     MNLLDKYLRQPGIAFTSSTAARDWLRLHLARQEREVFMVLYLDNQHRLLESETLFAGS
                     VNHVQVHSREVVKSALRFNAAAVVLAHNHPSGDPEPSQCDRNITGRLKEALELVDVKT
                     LDHLVVGRDGIVSFAERGWL"
     gene            complement(5740..6183)
                     /locus_tag="ACHKW0_004147"
     CDS             complement(5740..6183)
                     /locus_tag="ACHKW0_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7476887.1"
                     /translation="MNTQNLEFAEQAAITASAVPDELRIGFWPQHFGSVPQWITLESC
                     IFDWMDRLCTDYHGGIWSFLTLSNGGAFMAPESEHDEKWALFNSMNGNGAELTGEAAG
                     IVACLMTYSHHACRTECDAMTEHYYRLRDFALNHPECSAIMHLID"
     gene            complement(6214..7035)
                     /locus_tag="ACHKW0_004148"
     CDS             complement(6214..7035)
                     /locus_tag="ACHKW0_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7476888.1"
                     /translation="MRLASRFGRINQIRRDRPLTHEELMSHVPSVFGSDKHESRSDRY
                     TYIPTITILESLQREGFEPFFACQTKVRDQSKREHTKHMLRLHRAGQLTGHQVPEIIL
                     LNSHDGSSSYQMLPGLFRGVCTNGLVCGQSFGEVRVPHKGNVVEKVIEGAYEVLGVFD
                     RVEEKRDAMQSLALPAPARHALANAALEYRFGEDHQPVTVSQLLTPRRREDYSDDLWT
                     VYQRVQENLMKGGLSGRTAQGKSSRTRAVTGIDGDVKLNRALWVMAENMLEFFGR"
     gene            complement(7155..7628)
                     /locus_tag="ACHKW0_004149"
     CDS             complement(7155..7628)
                     /locus_tag="ACHKW0_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476889.1"
                     /translation="MRLPYVIVTLAMLASVSTAQAIPNMWTSGFGMGVTEYIITSPDK
                     VVFNLNCTGNPDAQNILQHGVDLTLPDGISVSSHDDGTEITVVMDNSQYPLPSFLGWR
                     NGDNAWVSFIDALSQAANFDVYVNDKKVGTFSPGLKNTQKELSDLSECRTTHYSD"
     gene            complement(7700..8152)
                     /locus_tag="ACHKW0_004150"
     CDS             complement(7700..8152)
                     /locus_tag="ACHKW0_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000734322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476890.1"
                     /translation="MKKLKLLSLLPVMFFPLLIAGNAHAARPYSGFLPAAVNNKSGNI
                     FWCDAHRQVRKLCTATEVDSMGWFIVVRSKPSRDCPGGIWGWITPSKDDVATGYTITP
                     GKEFNLTMKDMCSPDMKVTFRPNKKDPRYDDLVGIYAGKEAFRYKQIK"
     gene            complement(8188..8904)
                     /locus_tag="ACHKW0_004151"
     CDS             complement(8188..8904)
                     /locus_tag="ACHKW0_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012543038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7476891.1"
                     /translation="MTLQERRHDRLAVRLSLIISRLVSGETLFIRKLATEFGVSERTL
                     RRDFRERLMYLDLDYYQGQCSLRTDIHSTRQEIDILTFAHRTGLAGVFPGFDRRLVSS
                     LLDCDDRPCLVWHQDRPHPPAGKLSFYRLVRAISGCQRITILTEGERHEAVAPYRLIA
                     LDGYWYLVAEHNRQLRVFRLGEIHLVRPSRETFIRSELLYQLSEDKHFISALPHFRFI
                     QQSLNSFSPSESRGQSGQTD"
     gene            complement(9148..10023)
                     /locus_tag="ACHKW0_004152"
     CDS             complement(9148..10023)
                     /locus_tag="ACHKW0_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7476892.1"
                     /translation="MNKPDGLQAFEQPLASLPCTLRQLILERIQNLTHYEPVIGIMGK
                     SGTGKSSLCNELFQGEVSPVSDVNACTRDVLRFRLRSGRHSLVIVDLPGVGENGRRDH
                     EYRALYRRMLPELDLVLWVIKADDRALTLDEQFWLGVMQPYRQKVLFVINQADKIEPC
                     YEWDTLTSKPSTHQQGNLKAKQAAIMAMFKPHHPVCAVSASTGWGIEDMVAAMMRCLP
                     DRATSPVVTQLHGRLCTEPVKSQARDSFGDAVGRVFDTAESSSLLPAPLKKVIRTVRD
                     AVVSVARAVWDWIFF"
     gene            complement(10108..10992)
                     /locus_tag="ACHKW0_004153"
     CDS             complement(10108..10992)
                     /locus_tag="ACHKW0_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476893.1"
                     /translation="MVKLVGNDSSKIKYLENKLIENGYHFYSGGVDKDYREYQLRVFN
                     YLVSQNVSEQNINSFFAEVDNSYTRGFPSESELDWYRNDPRASLWLSCELYEKLKEET
                     PKYNIDFLSPEALQPDHNVRIEAIRHCMDEWPMYFTTPAEFIKDKSIEWAELLDQHDL
                     FRSVRSSKVDVCSWLRDYLRGNTSIGLKRICGNSSEEIMSWCYASYFIWRKNNLHSPD
                     SVELFIRKFKSAWSTQKNRNKNKEEKKLVTMSVNISQQAHDMLRDMSMKDSMSNNAII
                     ESAILRLYNIKNSKVRSK"
BASE COUNT         3044 a         2610 c         2581 g         2811 t
ORIGIN      
        1 aaaatacagc taccggttgc caaagacact ataagcctgg caaaaaaata ttacacaaca
       61 taaatgctaa ttgtttatgc gggctttgta ttgctttctg tatcctacaa atgagtgaaa
      121 tttatgaaaa aggctaaaat actttctggc gtattattac tgtgcttttc gtccccatta
      181 atttctcagg ctgcgacact ggacgtacgt ggtggatatc gtagtggaag ccacgcctat
      241 gagactcgac tcaaagtcag tgagggatgg caaaatggat ggtgggcaag catggaaagt
      301 aatacctgga ataccattca tgataataaa aaggaaaatg ccgcactcaa tgatgttcag
      361 gttgaagtta attacgcgat taaacttgat gatcaatgga cggtgcgccc gggaatgtta
      421 acgcatttta gcagcaacgg cacacgctac ggaccctacg taaaactgtc ctgggacgcg
      481 acaaaagatc ttaattttgg cattcgctat cgttacgact ggaaagctta ccgacaacaa
      541 gacttatccg gtgatatgtc tcgtgataac gttcatcgtt gggatggata tgtcacttac
      601 catattaata gtgatttcac cttcgcatgg caaacgacgc tatacagcaa acagaacgat
      661 tatcgctatg caaaccataa gaaatgggcg acggaaaatg catttgttct acaataccat
      721 atgacgcccg atattacgcc atacatagaa tatgactacc tggaccgtca gggtgtttac
      781 aacggcagag ataatttatc ggaaaacagt tatcgcattg gtgtgtcatt taaactgtag
      841 tagacaggag acagtcacaa tgaataaaac aataacggcg cttgctatca tgatggcttc
      901 atttgccgca aacgcgtctg tattacctga aactcctgtg ccatttaaaa gtggtaccgg
      961 agcaattgat aacgacactg tctacattgg tttaggtagc gcaggtacgg catggtacaa
     1021 gctggataca caggccaaag ataaaaaatg gacagcgtta gctgcattcc ctggtggacc
     1081 aagagatcaa gcaacctcgg catttattga tggcaatctg tatgtgtttg gcggcattgg
     1141 caaaaatagc aagggcttga ctcaggtatt taatgacgta cacaaataca accccaaaac
     1201 caatagttgg gttaaattga tgtcgcatgc accgatgggc atggcgggtc atgtgacttt
     1261 tgtacacaac ggcaaggctt atgttactgg cggtgttaac cagaatatct tcaatggcta
     1321 ttttgaagat ctcaacgagg ctggaaaaga ttcagcaact atagacaaga tcaatgccca
     1381 ttattttgac aaaaaagcag aagattattt cttcaataag tttctgttgt cttttgatcc
     1441 ctcaacacag caatggagtt acgctggcga atctccctgg tacggaacgg ctggtgcggc
     1501 gcttgtgaat aaaggtgata aaacctggct tattaatggc gaagccaaac caggattgcg
     1561 aacggatgcc gtatttgaac ttgatttcac cggtaataat ttaaaatgga ataagcttgc
     1621 tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg gggataagca atgattctct
     1681 tatatttgcc ggaggggccg gattcaaagg ttcacgagaa aattaccaga acggtaagaa
     1741 ctatgcgcat gaaggcctaa aaaaatcata tagcactgat attcatcttt ggcataacgg
     1801 gaaatgggat aaatcgggtg aattatcgca aggtcgggcc tacggagtat cattgccctg
     1861 gaataatagt ctattgatta ttggcggtga aactgcaggc ggcaaagcgg tgacggattc
     1921 agttttgatc tctgtgaagg ataataaagt cacagtacaa aattaacgct tcagggcccc
     1981 ggtaaagggg ccctgctatc aatttgtcat ttcaattacg cgaaatttat atgaacgcaa
     2041 taatatcgcc cgattattac tatgttctta ccgttgctgg tcagtctaat gccatggcgt
     2101 atggcgaagg actgccatta ccggacaggg aagatgcgcc tcatcccaga attaaacaat
     2161 tagcgagatt tgcgcatacg catcccggag gcccgtcatg tcactttaac gacattattc
     2221 cactgactca ctgcccacac gatgttcagg atatgcaggg ttatcaccat cctctggcaa
     2281 cgaatcatca aacacagtac ggcaccgttg gccaggcact gcatattgca cggaaattac
     2341 taccctttat tcctgataat gcaggggttc tcatcgttcc atgttgccgt ggcggatcgg
     2401 cttttaccgc gggcagcgaa gggacatatt cagaacggca cggagccagc catgatgctt
     2461 gtcgttgggg aacggatact ccgctgtacc aggatttagt cagcagaacg cgagccgcac
     2521 tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg gatgcaaggc gaatttgact
     2581 taatgaccag tgactacgcg tcacaccctc aacactttaa tcatatgatt gaagcctttc
     2641 gtagggatct aaaacaatac cattctcagc ttaataatat tactgacgcg ccgtggtttt
     2701 gcggcgatac cacctggtac tggaaagaaa atttccctca tgcgtatgaa gttatttatg
     2761 gcaattatca aaataatgtt ttagccaata ttattttcgt cgacttccag caacaaggtg
     2821 aaagaggact gacgaacgcg cctgatgaag acccggacga tttaagcacg ggatattacg
     2881 gttcagcgta ccggtcaccg gagaactgga cgacggcact gcgaagcagt catttcagcg
     2941 cggcagcccg tcgggggatt atttctgaca ggtttgtaga agcaattttg cagttttggg
     3001 gcgaaaagtg agcgttcatt aatttgattt attcgcatcc atcaacgctt ttaactcctc
     3061 cggcgtaaac ggtggttagc gtcggtgcag catcgcatgg caattagggt acacaggcag
     3121 caaaaaaaca aatgagatca aagtaatata tcttcacttg caggcttgaa cagccgaaaa
     3181 tttgcgagca tgatcgcaat tatttcactg ccagggatat caatggcttc cagtaaatcg
     3241 ctacagcagg caattgccaa tataaaaatc tggcacaagg gtgaacagcg cgcgccgcat
     3301 aagccattgt tattgctata cgtattagcg ggatacctga atggacatcc acgccttttc
     3361 gattattgta gtggaacagt ctacgaggat gcagtttgaa gtgagtaagt aaaggttgct
     3421 ggaaacgatc aatgccgcca tacaatgata tttgaggatc accaatgagt aataaaaact
     3481 acgaaagtca ccgcaaagca atagttagta aaggtatacc acccactcta ttaaataggc
     3541 tcaccaattc ggatgttcag gtgatcaata ccttcttaac acgagtgagc aaactggaac
     3601 tatctcaaca agagaaagac tggatcataa aaatcatctc tatggtttag agaatttcag
     3661 ccttgtagcc tttcaagggt tttgtgtggg tacaaacatt atcgaatcgg gatatagccg
     3721 cagctgtagc tgtatccaca tcagtaagtt gatgtaggtt gacttaggtt gagcgaaaaa
     3781 tagcgaaaag ccttgtgtgg cgcggatttc agtcataaaa aaacgtccgt tgacgtctat
     3841 tgatgttccg atggtgcgaa ggtgcgaagg tgcgaaggcc ggactcgcac attacaatta
     3901 actccttgaa ttaaaataaa taaaaacaaa ctaattttat gcgtaccaac gcagatacca
     3961 acacattaat tcattggatt cgggactcga atcagggcaa agccaagtga acaaaattcc
     4021 cctatgagta gatgatacag ataaagatga gaagctggct ttagccttcg cgaactcctc
     4081 atcatctggt tactgttagc tttccctaca ttggacagca ttgtatcaat gttgtcgata
     4141 tcaacattaa ttgacttccc atagcttata gctcctgaag tcgacctgcc gttaccctgg
     4201 taacagtttt gtgactgttg cgtttcatca gaccggaggc tttccgggca cgtagaatat
     4261 cgatactggt gatgaacggt gacttctcca tgacagagca atcattacag tcaattctta
     4321 ccagttcata tttttcaaca agaaagttca ccgcatcgca cagcgatact ccagcatcaa
     4381 tgtgttccag tatggtggtt tcattactga acggtgtgtc attcaacgtc agaccatagt
     4441 gctgcgacaa cagacaagtc agtaaccgct gccagatttc aacgggtgac aggcagggct
     4501 gagtcgcccg tgtcgggtgt gatgatatag tttgcatgat gtttgctctg tgagtttaag
     4561 aggacaagcg gggatttact gcggctcagt ttgctcagga tagatagcaa tgtagacata
     4621 accacaggag cctagtgtat cggcttcaca ggtgagtcca ttgtggtaaa gcgtgacgca
     4681 gtgtgggtgg cgagggttaa gctctccact ggtcagcatc agctcaagtt gcttcatcat
     4741 taacggaaat gcctggtcaa ggcgcattgc aacatcgacg gcaaatgccc cgctaaatcc
     4801 tgcgcggtca gagagaaaat gcaatcggtt gccctcctgt accaggcggg caccaaaaca
     4861 gggggtaata gcacatttaa gcccccactg ctggcaggcg ggattctccg gctttgtacc
     4921 ggattctgag tgattgttca ttattgttcc tgattaaagg gaaggattaa ttaagagtaa
     4981 tgcttcgaag aatgacatag ggaccattgc ggtcttgacg acgttcagca aatacagcca
     5041 gaacgccgcg tatgtcctga acttcccggc cagcataatg gcagatactg ccggaccatt
     5101 tatatttgcc ggtacagaag cgaaacagcc gagagcctgg atgctgtcgg tacaaagcca
     5161 ttgcctgacg tttactgatg attttcataa ccaacctcgc tcagcgaacg acacaatgcc
     5221 atcccgaccg acaacaagat gatcgagcgt tttcacatca accagttcta acgcttcttt
     5281 aagcctgccc gtaatgttgc gatcgcattg gctgggctcc ggatcaccgg agggatggtt
     5341 atgcgccagc acgacagctg cggcattgaa acgaagcgcc gacttcacta cctcacggga
     5401 atgaacctgc acatggttta ccgaaccggc gaacagcgtt tcactctcca gtaaacgatg
     5461 ctgattatcg aggtagagca ccataaacac ctcccgctcc tgtctggcca ggtgtaaccg
     5521 gagccagtca cgggctgcag tgcttgaggt aaaggctata ccgggctgac gcagatattt
     5581 atccagcagg ttcatagcgc ggcgaatggt ccgttgggcg tagggtgtca gtccggaagt
     5641 cgtagggagt aaacagccat gctgagccat gaccgacacc ggaaaaagct caggctgcga
     5701 taacagtggg gatattgtgg tattcatatt cggtatgact cagtcgatga gatgcataat
     5761 tgcgctgcac tctggatggt tcaacgcgaa gtctcgtaag cgataatagt gctcagtcat
     5821 cgcgtcacac tcggtgcgac aagcgtggtg gctgtatgtc atcagacagg cgactatccc
     5881 tgcggcttcg ccagtaagtt ccgcgccgtt gccgttcata ctgttgaaca atgcccactt
     5941 ctcatcgtgc tctgactcag gagccataaa agcgccgcca ttgctgagcg tcaaaaagct
     6001 ccagataccg ccgtgataat cggtacacag gcggtccatc cagtcaaaga tacaggactc
     6061 cagggttatc cattgcggaa cggagccaaa gtgctgcggc cagaagccga tgcgtaactc
     6121 gtcagggacc gctgaggcag ttattgctgc ctgctcagcg aactcaagat tttgtgtatt
     6181 cattaaacta tctccttatg caggaactgt tgtttaacgc ccgaaaaact cgagcatgtt
     6241 ttctgccatc acccacaggg cgcgattgag cttcacatcg ccatcaatac cggtaacagc
     6301 acgggtgcgg ctgcttttcc cctgagcggt tcgccctgac agaccacctt tcatcaggtt
     6361 ctcctgcacg cgctggtaga cggtccacag gtcatcgctg tagtcctccc ggcgacgtgg
     6421 ggtcagcaac tgcgataccg tgaccggctg gtggtcctca ccgaagcgat attccaacgc
     6481 agcatttgcc agcgcgtgac gggccggggc tggcagcgcc agtgactgca ttgcatcgcg
     6541 cttctcctcc acccggtcaa acaccccgag cacttcgtaa gccccttcaa tcaccttctc
     6601 aacgacattg cctttatgtg gcacccgtac ctctccgaac gactgaccgc agaccaggcc
     6661 gttggtacaa acgccacgaa acagccccgg cagcatctgg tagcttgatg agccgtcgtg
     6721 gctgttaagc agaatgattt ccggtacctg atgcccggta agttgaccgg cacgatgcag
     6781 gcgaagcatg tgtttggtgt gttcccgctt gctctggtca cggacctttg tctggcaggc
     6841 aaagaacggc tcaaagcctt cacgctgcag gctttcgagg atggtgatgg tcgggatgta
     6901 ggtataacgg tctgaacgag attcatgctt gtcactcccg aacacgctgg gtacatgact
     6961 catcagctct tcatgggtca gaggccggtc acggcgtatc tggtttatac gaccaaagcg
     7021 gctggctaat cgcatagcta actccttatc tgataatgaa ataaaaacaa aaaggccatg
     7081 ttcccgaagg agcatggcct gttgctatct ggtgttggta tgttgtgggg tggagaggtc
     7141 tgaaatgtaa gtaactaatc gctgtagtgt gtggtccggc actcactgag gtcagacagc
     7201 tctttttgcg tattcttcag ccccggactg aaggtgccga cttttttatc gttgacgtaa
     7261 acatcaaaat tcgcagcctg gctcagggcg tcaataaatg acacccaggc attgtcaccg
     7321 tttcgccagc cgaggaatga cggtaacgga tactgactgt tatccatgac gaccgttatt
     7381 tccgtcccgt cgtcatgaga gcttactgat attccatcag gcagggtgag atcaacaccg
     7441 tgttgcagaa tattctgcgc atccggattg ccagtacagt tcagattaaa caccacctta
     7501 tccgggctgg taatgatata ttcggtcaca cccatcccaa aaccactcgt ccacatattg
     7561 ggaatagcct gtgctgttga tacagaagcc agcatagcta atgtaactat tacataaggc
     7621 agtcgcataa tgttgtcctc aaatcatgta ataaagcttt tgttgtttaa aacaataaag
     7681 taacgaaaaa acagttggat tattttattt gtttgtagcg aaacgcttct ttccctgcat
     7741 aaatcccgac gagatcgtca taacgagggt cctttttatt cgggcggaac gttactttca
     7801 tatcgggact gcacatatcc ttcatggtga ggttgaattc tttacctggc gtaatggtat
     7861 aacctgttgc cacatcgtct ttactgggag tgatccaccc ccatattcca cccggacaat
     7921 ctctggaagg tttcgagcga acaacaataa accatcccat tgaatccact tccgtcgctg
     7981 tacacagttt tctgacctgt cggtgggcat cacaccagaa tatattccca cttttattat
     8041 tcactgccgc aggcaggaac ccgctataag gacgtgctgc gtgagcatta cctgcgataa
     8101 gtaagggaaa aaacatgact ggcagtaaag acaataattt caattttttc attagattaa
     8161 ccttacattc aattgtggag accatctcta atcagtttgt ccggattgcc cccgactttc
     8221 tgatggagaa aatgaattca gcgactgctg aataaaacgg aaatgaggta aggcagagat
     8281 aaaatgtttg tcctccgaca gctgatagag aagctcactg cgaataaatg tttcccgcga
     8341 tggccttacc agatgaatct cacccagccg gaagacacgt aattgcctgt tatgttcagc
     8401 gacaagatac cagtaaccgt ccagagcgat tagccggtaa ggtgcaacgg cctcatgacg
     8461 ctccccttca gtcagaatgg tgatgcgctg acatcccgaa atagctctga ccagcctgta
     8521 gaaggagagc tttcctgccg ggggatgagg tctgtcctga tgccatacca gacagggtcg
     8581 atcatcacag tccagcaggc tgctgaccag tcgccggtca aagcccggga aaaccccggc
     8641 cagccccgtg cggtgagcaa aggtcagaat atctatttcc tgccgcgtac tgtgaatatc
     8701 cgtgcgcaga ctgcactgcc cctggtagta atccaggtcg agatacatca gacgttcccg
     8761 aaagtcacga cgcagcgtgc gctctgacac cccgaattca gttgccagtt tgcgaataaa
     8821 cagagtttca ccggacacca ggcggctgat aatcaacgac aacctgacgg caagccggtc
     8881 atgtcgccgc tcctgaagcg tcatgaaaga tttctcctga gaagttaact ctatgaatta
     8941 ataatgatta ctttaaagga aggagtggac agggtatgga caacatcgga actatttttt
     9001 atgagcagtc cagccttgtg ggcgctcgtc tgacagataa tgatgcttcg cctgatttaa
     9061 ttgatgataa aacgcagtgg aaaagacgtg tccacaatca acacatcatt aacaaccagt
     9121 tcatcattcg cagagaaaat tgcgatatca gaagaatatc cagtcccaga cggctcgtgc
     9181 gaccgacacc accgcatcac gcacggtacg aatcaccttt ttcagtggcg caggcagtaa
     9241 tgacgatgac tctgccgtat cgaaaacacg gcccacagcg tcaccgaaac tgtcacgggc
     9301 ctgcgacttt accggctctg tgcagagtct cccgtgcagt tgtgtcacca ccgggctggt
     9361 ggcgcggtca ggcagacagc gcatcatggc tgccaccata tcctcaatgc cccatccagt
     9421 gctggcggac accgcacaga ccggatgatg tggtttaaac atcgccataa tcgcagcctg
     9481 cttcgcttta agatttccct gttggtgtgt tgaaggtttg ctggtgaggg tatcccattc
     9541 atagcagggt tcgattttgt cggcctggtt aatcacaaac agtacctttt gcctgtaagg
     9601 ttgcatcaca ccgagccaga actgctcgtc caaggtcagt gcccgatcat cggccttaat
     9661 gacccacagc accaggtcca gctccggcag cattcgacgg tacagggccc ggtactcatg
     9721 atcccgtcgc ccgttttcac ctacacccgg taaatcgaca atcaccagac tgtgacgacc
     9781 gctgcgcaac cggaagcgca gtacgtcgcg agtacaggcg ttcacgtcac tcactgggga
     9841 cacctcgccc tggaaaagtt cgttgcagag agaggattta cctgtaccac ttttgcccat
     9901 gattccaatc accggctcgt aatgggtcag gttctggata cgttcaagaa taagctggcg
     9961 aagtgtgcac ggaagcgacg caagcggttg ttcaaaggcc tgaaggccgt caggtttatt
    10021 catacagtag tctcagcgtg aaaaattaaa aaaccccgcg atccagtcag gaaagcgggg
    10081 gttcacgtag ataatatctg tctgaaatta tttggaacgt actttactat tcttaatatt
    10141 atataaccta aggatagcac tctctataat tgcgttattt gacatcgagt ctttcataga
    10201 catatctctc agcatatcat gagcttgctg agagatattt acactcattg tcacaagttt
    10261 tttctcttct ttatttttat tcctattttt ttgagtagac caggcgctct taaactttct
    10321 aatgaacaac tcgacagaat ctggagaatg caggttgttt tttctccaaa taaagtaact
    10381 tgcatagcac cacgacatta tttcctcgga agaattacca caaatacgtt tcagacctat
    10441 tgatgtattc ccccttaagt aatctcttag ccaagaacag acatcgacct tactggatct
    10501 aacacttctg aataaatcat gttgatcaag taactcagcc cactcaatac ttttatcctt
    10561 aatgaattca gccggtgttg taaaatacat aggccattca tccatacaat gacgtatcgc
    10621 ttcaattctc acattatgat caggttgcaa tgcttctgga gatagaaaat caatattgta
    10681 cttaggagtt tcttccttta atttctcata taactcacaa gacaaccata agcttgctct
    10741 tggatcatta cgataccaat caagttcact ctcggatgga aaacctcttg tatagctatt
    10801 atccacctca gcaaagaaag aattaatgtt ctgctcagaa acattctgtg aaacaaggta
    10861 attgaaaacc cttaactgat attcccggta atctttatca accccaccag aatagaagtg
    10921 ataaccattc tcaattaatt tattttcaag atatttaatc ttactactgt cattccccac
    10981 tagcttaacc ataccggcac cttccttgat atcactaagt gatttacagt gaattaatga
    11041 ctttta
//
LOCUS       DAZFXT010000147        10973 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.147, whole genome shotgun sequence.
ACCESSION   DAZFXT010000147 DAZFXT010000000
VERSION     DAZFXT010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10973
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.147"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(194..2461)
                     /gene="adiA"
                     /locus_tag="ACHKW0_004154"
     CDS             complement(194..2461)
                     /gene="adiA"
                     /locus_tag="ACHKW0_004154"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HFO7476894.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEVTPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2660..3568)
                     /gene="melR"
                     /locus_tag="ACHKW0_004155"
     CDS             complement(2660..3568)
                     /gene="melR"
                     /locus_tag="ACHKW0_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HFO7476895.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRAHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3851..5206
                     /gene="melA"
                     /locus_tag="ACHKW0_004156"
     CDS             3851..5206
                     /gene="melA"
                     /locus_tag="ACHKW0_004156"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HFO7476896.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5309..6730
                     /gene="melB"
                     /locus_tag="ACHKW0_004157"
     CDS             5309..6730
                     /gene="melB"
                     /locus_tag="ACHKW0_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HFO7476897.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6869..7498)
                     /gene="yjdF"
                     /locus_tag="ACHKW0_004158"
     CDS             complement(6869..7498)
                     /gene="yjdF"
                     /locus_tag="ACHKW0_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HFO7476898.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7621..9267)
                     /gene="fumB"
                     /locus_tag="ACHKW0_004159"
     CDS             complement(7621..9267)
                     /gene="fumB"
                     /locus_tag="ACHKW0_004159"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HFO7476899.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9345..10685)
                     /gene="dcuB"
                     /locus_tag="ACHKW0_004160"
     CDS             complement(9345..10685)
                     /gene="dcuB"
                     /locus_tag="ACHKW0_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HFO7476900.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
BASE COUNT         2572 a         2681 c         2906 g         2814 t
ORIGIN      
        1 ttatagtaat ttctcatgta catatttttt tacaggataa ataacaggca aaaaaaagcg
       61 cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt
      121 tgccatactc aaaaggccta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc
      181 agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag
      241 tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta
      301 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
      361 ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg
      421 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgacttccg
      481 ccaccggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc
      541 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
      601 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
      661 aggagcaaag ggtgttaacc agagttcccc atttcccacg ggttacgccc atagagaaca
      721 ggaacataat ttggaagtca gtggtgcggg taggtacaat gccgtggcga ccaagccagg
      781 cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
      841 gagcaaggat gctgacttta atcgggtcga gcatactcca gttatccgga atatctttga
      901 agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt
      961 tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
     1021 tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatat agccgcgcca
     1081 tcgcctgacg gaaatcaacc gcttcgtcaa tcacttcctg tgtcagtgac aggccgctgt
     1141 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
     1201 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
     1261 cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg
     1321 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
     1381 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
     1441 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc
     1501 agttggtcac cacgcagtaa gacggttttt gcccggcttt gtctttggtc agcgggcttt
     1561 cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
     1621 tgccgtagcg gttgcggctt ggcaccatat agaccggttt cgcgcctgtc agcatcaaac
     1681 cttgttcgat ggatttatgg cagttacggt caacgaccac gacatcgtta tcggtcatgc
     1741 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
     1801 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
     1861 gcaaagaacc gagggaagtt cgttcgatgc ccatgtcggt gcggaacaga ttttcaccat
     1921 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
     1981 ctggcgctgc ccaggaatat tcatggatgt cactatattt catcagcgcg ctgaacagtg
     2041 gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac ggcgcgtccg gcgataaagt
     2101 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcagg tcgcgatcca
     2161 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
     2221 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
     2281 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
     2341 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
     2401 cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
     2461 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
     2521 gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttatttttat aaacaatgcg
     2581 ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac tatttttttg
     2641 gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc
     2701 ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg
     2761 aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg
     2821 cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc
     2881 gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga
     2941 tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt
     3001 gcgcatggcg cgagacgctg tttttgtgtg cgcgcgaggt tttattgacc agaatcggtt
     3061 cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc
     3121 gaatttgctc gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa
     3181 gttgctgtgt ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt
     3241 tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac
     3301 aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct
     3361 gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat
     3421 tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa
     3481 tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc
     3541 tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta
     3601 tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag
     3661 aggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt
     3721 accataaact cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac
     3781 ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca
     3841 ggagatctgc atgatgactg cacccaaaat tacatttatc ggcgctggtt cgacgatttt
     3901 cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc
     3961 cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagctgat
     4021 ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaca
     4081 ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac
     4141 tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc
     4201 gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat
     4261 gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac
     4321 ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc attcggtgca
     4381 gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccagctacgc tgcgttaccg
     4441 ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg
     4501 cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa
     4561 gccgaatatt catggcaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa
     4621 gctgggctac ttcgtcacgg aatcgtcaga acattttgct gagtacacac cgtggtttat
     4681 taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa
     4741 acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc
     4801 ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac
     4861 tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc
     4921 acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccgaccaa
     4981 agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac
     5041 gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat
     5101 ggacccgcat actgctgccg tgctgggcat tgatgaaata tatgctcttg ttgacgacct
     5161 gattgccgcc cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg
     5221 ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg
     5281 ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta
     5341 tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta
     5401 ttactacacc gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc
     5461 gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc
     5521 gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt
     5581 atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt
     5641 gacctacatc ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt
     5701 tccaaccatc acgctcgata aacgtgagcg cgaacaactg gttccttatc cgcgtttttt
     5761 tgccagtctg gcaggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg
     5821 cggtggcgat cggggatttg gctttcagat gttcactctg gtactaatcg ccttttttat
     5881 tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc
     5941 gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgctaattt ataaaaacga
     6001 tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac
     6061 cggatttgct atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta
     6121 tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa
     6181 atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg
     6241 tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg
     6301 tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga
     6361 taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt
     6421 gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt
     6481 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
     6541 gtttattatg attgctctac caactctgtt tttcatggta acgctgattc tctacttccg
     6601 tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata
     6661 tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
     6721 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc
     6781 acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc
     6841 gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct
     6901 ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga
     6961 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
     7021 ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg
     7081 ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga
     7141 gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat
     7201 acggattacg gctcaacccc agccattcct gcacctcaaa accgatgggg actttcgcgt
     7261 aggtgtattg cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca
     7321 gcggcgttaa cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg
     7381 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga
     7441 ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg
     7501 tgagttcgca agtcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     7561 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     7621 ttacttagtg cagttcgcgc actgtttgtt gacgatctgc tggaagaagt cgttaccttt
     7681 gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     7741 acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc
     7801 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac
     7861 ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag
     7921 atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataacc
     7981 ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag
     8041 ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat
     8101 aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct gggcgaggat
     8161 ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag
     8221 ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg
     8281 gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca
     8341 ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc
     8401 aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac
     8461 atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgagc
     8521 gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc
     8581 gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt
     8641 tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc
     8701 agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag
     8761 gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc
     8821 attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc
     8881 ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt
     8941 gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa
     9001 ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac
     9061 ctgtttctgg tgtgccggac ggagcatgaa agaagcgtcg tgaaaggctt gctgcgccag
     9121 tagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct
     9181 aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc
     9241 ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc
     9301 gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac
     9361 atcgcggcga agatccagcc gaagacgcac gatacgctaa caccaatcaa ccccggcaga
     9421 ataaaactgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga
     9481 atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct
     9541 gaagccacga tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc
     9601 agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcatacgcc
     9661 cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg
     9721 aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa
     9781 cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc
     9841 gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg
     9901 aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat tgccccgagg
     9961 aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatcgagcag cgtcgcggta
    10021 tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct
    10081 ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac
    10141 gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg
    10201 acattaccca gcattgcaac cagagacacg accgcaaccg acaccggact ggcgataatc
    10261 cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta
    10321 atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga
    10381 atggtcagtg tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc
    10441 agcagcttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc
    10501 gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga
    10561 aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata
    10621 ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat
    10681 aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac
    10741 gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg
    10801 ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa tttatgcact
    10861 gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg
    10921 cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc tat
//
LOCUS       DAZFXT010000148        10925 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.148, whole genome shotgun sequence.
ACCESSION   DAZFXT010000148 DAZFXT010000000
VERSION     DAZFXT010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.148"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..465
                     /locus_tag="ACHKW0_004161"
     CDS             <1..465
                     /locus_tag="ACHKW0_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
                     /protein_id="HFO7476901.1"
                     /translation="ISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRS
                     IIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRR
                     GNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            734..2971
                     /gene="nrfB"
                     /locus_tag="ACHKW0_004162"
     CDS             734..2971
                     /gene="nrfB"
                     /locus_tag="ACHKW0_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-3',5'-guanylate-activated
                     glycosyltransferase NrfB"
                     /protein_id="HFO7476902.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            2958..5930
                     /gene="nfrA"
                     /locus_tag="ACHKW0_004163"
     CDS             2958..5930
                     /gene="nfrA"
                     /locus_tag="ACHKW0_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HFO7476903.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            5931..6821
                     /locus_tag="ACHKW0_004164"
     CDS             5931..6821
                     /locus_tag="ACHKW0_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HFO7476904.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            7004..7765
                     /gene="envY"
                     /locus_tag="ACHKW0_004165"
     CDS             7004..7765
                     /gene="envY"
                     /locus_tag="ACHKW0_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HFO7476905.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRVLLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            8278..9231
                     /gene="ompT"
                     /locus_tag="ACHKW0_004166"
     CDS             8278..9231
                     /gene="ompT"
                     /locus_tag="ACHKW0_004166"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HFO7476906.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            9418..10902
                     /locus_tag="ACHKW0_004167"
     CDS             9418..10902
                     /locus_tag="ACHKW0_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7476907.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHYLITPQKIENIYCPGNSEAEIQ"
BASE COUNT         2955 a         2696 c         2645 g         2629 t
ORIGIN      
        1 atcagtgaaa agagtcacgg cagagaagaa atccgtcttc atattgtttg cgatgtccct
       61 gatgaactta ttgatttcac gtttgaatgg aaaggtctga agaaattatg cgtggcagtc
      121 tcctttcggt caataatagc agaacaaaag aaagagcccg aaatgacggt cagatattat
      181 atcagttctg ctgatttaac cgcagagaag ttcgccacag caatccgaaa ccactggcac
      241 gtggagaata agctgcactg gcgtctggac gtggtaatga atgaagacga ctgcaaaata
      301 agaagaggaa atgcagcaga attattttca gggatacggc acatcgctat taatattttg
      361 acgaatgata aggtattcaa ggcagggtta agacgtaaga tgcgaaaagc agccatggac
      421 agaaactatc tggcgtcagt ccttgcgggg agcgggcttt cgtaatcttg ccctgcctgc
      481 tcacctcctc caggcacttc cactacatga tgacctgatc ggtgccacca ctcctcttca
      541 ctatgacgtt aacctcccct ccttgacacc acgcttttgt ctacattcta cgcaaacggc
      601 aacttttata accagtgtaa aaataacgtg ccgcaatagt catacctatt aatggtaaaa
      661 agctgtcaca attcataaaa aaccttaata tacgccaccc taaacataac cagcgttaat
      721 gtaaggtttt tgtgtggact ggcttcttga tgtttttgca acctggctct acggcttaaa
      781 agtaatcgcg ataacgttag cggtcatcat gttcatcagc ggactggacg atttttttat
      841 tgatgtcgtc tactgggtac gccgcattaa acgcaagttg agtgtttatc gccgctaccc
      901 gcgaatgagt taccgcgaac tgtataaacc agatgaaaaa ccgttagcga ttatggttcc
      961 ggcgtggaat gaaacgggcg tcatcggcaa tatggccgag ctggcggcga ccacgctcga
     1021 ctacgaaaac tatcatatct ttgttggcac ctaccccaac gaccccgata ctcaacgtga
     1081 tgttgacgaa gtgtgcgctc gctttccgaa cgtgcataag gtagtctgcg cgcgtcctgg
     1141 tcccaccagc aaagccgact gtctgaacaa cgtgctggac gccatcaccc agtttgaacg
     1201 tagcgccaat ttcgcttttg ctggttttat tctgcatgac gccgaagatg tgatttcacc
     1261 gatggaattg cgtctgttca actatctggt cgagcgtaaa gatctgattc agatcccggt
     1321 gtatccgttc gaacgcgaat ggacgcactt caccagcatg acttacattg atgagttttc
     1381 agagctgcat ggcaaagatg ttccggtgcg tgaagccctc gccggacaag tgcccagcgc
     1441 aggcgtcggc acctgtttca gccgccgcgc cgtgaccgct ctgttagctg acggtgacgg
     1501 tattgctttc gacgtgcaga gtctgactga agattacgac attggtttcc gcctgaaaga
     1561 gaaaggtatg acggaaattt ttgtccgttt tccggtggtg gacgaagcca aagaacgcga
     1621 gcagcgtaaa tttttacagc acgcacggac gtcaaacatg atctgcgtgc gcgaatattt
     1681 ccccgatacc ttttcgactg cagttcgaca aaaatctcgc tggatcatcg gcattgtttt
     1741 ccagggcttt aaaacccaca aatggacctc cagcctgacg ctgaactact ttctctggcg
     1801 cgaccgcaaa ggggcaatca gtaactttgt cagcttcctc gcgatgctgg tgatgctcca
     1861 gcttttgctg ttgctggcgt atgaaagttt gtggcccgat gcctggcatt tcctttctat
     1921 ttttagcggc agcgcatggt taatgaccct gctgtggcta aactttggct tgatggttaa
     1981 ccgcatcgtg cagcgggtga ttttcgtcac tggctactac ggtctgacgc aggggctgtt
     2041 atctgtcctg cgtcttttct ggggcaacct gattaacttc atggccaact ggcgcgcgct
     2101 aaaacaggta cttcaacacg gcgatccacg tcgcgtcgcg tgggataaaa caacgcatga
     2161 cttccccagc gtgactggcg atacccgctc gttgcgcccg ttaggccaaa ttctgctgga
     2221 aaatcaggtc atcactgaag aacaactcga tacagcactg cgtaatcgcg tcgaaggtct
     2281 acgcttgggc ggttcaatgc tgatgcaggg gctgattagc gccgagcagc tggcacaggc
     2341 gctggcagag caaaacggcg tggcgtggga atccatcgat gcctggcaga tcccgtcctc
     2401 gctgattgcc gaaatgccgg cctccgtagc gctgcattat gcagtactgc cgctgcgtct
     2461 ggaaaatgat gagttaattg tcggcagtga agatggtatt gacccggttt cgctggcggc
     2521 cctgacgcgt aaagtcggac gcaaagtgcg ttacgtcatt gttctgcggg gacaaattgt
     2581 cacggggtta cgtcactggt atgcacgccg acgcggtcac gatccgcggg caatgttgta
     2641 caatgcggtt cagcatcagt ggctcacgga acagcaggcc ggtgaaatct ggcggcaata
     2701 tgtgccgcat cagttcctgt tcgccgaaat actgaccacg ctcggtcata ttaatcgttc
     2761 agcaattaac gtgttgttat tgcgccatga acgcagttct ctgccgctcg gcaagttttt
     2821 ggtcaccgaa ggcgttatca gccaggaaac gttggatcgc gtcctgacaa ttcaacgcga
     2881 attacaagtt tcgatgcaat cactattact caaagcaggt ttaaacacag aacaggttgc
     2941 gcaactggag tccgaaaatg aaggagaata accttaatcg cgtcatcgga tggtctggtt
     3001 tactgctgac gtctttattg agtaccagcg cactcgcaga caatatcggc accagcgcag
     3061 aagagctggg gctgagcgat tatcgccatt ttgttattta tccccgtctc gataaggcgc
     3121 tgaaggcaca gaaaaataac gacgaagcaa ccgccatccg cgaatttgaa tatatacacc
     3181 agcaggtgcc ggataatatt ccgctgactt tataccttgc ggaagcctat cgccattttg
     3241 gtcatgatga ccgggcgcgg ctgttgcttg aggatcaact gaaacgtcac ccaggagatg
     3301 cccgacttga gcgcagtctg gcggctattc cggttgaagt gaaaagcgtt acgactgttg
     3361 aagaactgct tgcccagcaa aaagcgtgcg acgctgcgcc gaccctgcgt tgtcgcagtg
     3421 aagtcgggca gaatgccctg cggctggcac aattacctgt cgccagagcg caactgaacg
     3481 atgcgacgtt tgctgcatcg ccagaaggaa aaactctgcg aaccgatctg ctgcaacggg
     3541 caatctacct gaaacaatgg tcccaggcag atacgctata caatgaagca cgccagcaga
     3601 acacattaag cgcggcagaa cgccgtcagt ggtttgacgt gcttcttgcc gggcagctgg
     3661 acgatcggat cctggcactg caatcacagg ggatcttcac cgatcctcag tcatatatta
     3721 cttacgcgac cgcgctggct tatcgtggcg aaaaagcacg cctccagcat tatctcaatg
     3781 aaaataagcc gctgtttacc acggacgcac aagagaaaag ttggctctat ctgttatcta
     3841 aatacagcgc caaccccgtt caggcattgg cgaattatac ggtgcagttt gccgataacc
     3901 gccagtatgt tgttggcgcg acgctaccgg tgctgttaaa agaaggtcag tacgacgcag
     3961 cgcaaaaact gctcgccacc ctccccgcca atgaaatgct tgaggagcgt tatgctgtca
     4021 gcgtggcgac ccgtaacaag gctgaatctc tgcgtctggc acgattgctg tatcagcaag
     4081 aaccggcaaa tcttacccgc ctggatcaac tgacctggca actgatgcag aacgagcagt
     4141 cacgcgaagc tgccgattta ttgctgcaac gctatccttt ccagggcgat gcgcgtgtca
     4201 gccagacttt aatggcgcga ctggcgtctc tgctggaaag tcatccttac ctggcaacgc
     4261 cggcgaaggt ggcgatttta tcgaaaccct taccgctggc ggagcaacgt cagtggcaaa
     4321 gtcagttgcc gggtattgca gataattgcc cggcaatagt tcgcttgctg ggcgatatgt
     4381 cgccttccta cgatgccgcc gcctggaacc gtctggcaaa gtgttatcgg gacacgctac
     4441 ccggtgtggc gctgtatgca tggcttcagg ccgaacaacg acaaccgaac gcctggcaac
     4501 atcgtgcggt agcctatcag gcgtatcagg ttgaggacta cgccaccgca ctggcggcct
     4561 ggcagaaaat cagtcttcac gacatgagca atgaggatct gcttgctgct gccaataccg
     4621 cccaggcggc aggaaatggt gcagctcgcg atcgctggct acaacaggca gaacaacgtg
     4681 gactgggaaa caatgccctc tactggtggc tgcatgcgca acgttacatt cctggtcagc
     4741 cggaactcgc actgagcgat ctcacgcgct caatcaatat tgccccttct gccaacgctt
     4801 acgttgcgcg ggcgacaatt tatcgccaac gtcataatgt cccggcggcg gtgagtgatt
     4861 tgcgcgccgc gctggaactg gaaccgaata atagcaacat ccaggcagcg cttggttacg
     4921 ccttgtggga tagcggtgat atcgcacagt cgcgggaaat gctcgaacag gcgcataaag
     4981 ggcttccgga cgatccggca ctgatccgac aactggccta cgtgaaccag cgtctggatg
     5041 acatgcctgc gacgcagcac tacgcccggc tggtgattga tgacattgat aatcaggcgc
     5101 tgataacccc actgacccca gagcaaaatc agcaacgctt caatttccgc cgtttgcatg
     5161 aggaggtcgg tcgccgctgg acgttcagtt tcgattcttc tatcggcttg cgttccgggg
     5221 caatgagcac cgctaacaat aatgtcggcg gcgcagcgcc agggaaaagc tatcgtagct
     5281 acgggcaact ggaagccgag taccgcatcg gacgcaatat gctgctggaa ggcgacctgc
     5341 tctcagttta tagccgcgtc tttgccgata ccggagaaaa cggggtgatg atgccggtga
     5401 aaaatccgat gtccggcacc ggtctgcgct ggaagccgct gcgcgatcag atctttttcc
     5461 tcgccgtcga acagcagttg ccgctgaacg gccaaaatgg cgcatccgat accatgctgc
     5521 gcgccagcgc ctcattcttt aatggcggca aatacagcga cgaatggcac ccgaacggtt
     5581 caggctggtt tgcccaaaac ctgtacctcg atgcggcgca atatatccgc caggatattc
     5641 aggcgtggac ggcagattat cgcgtcagct ggcatcagaa ggtagctaac ggacagacta
     5701 ttgagcctta cgctcacgtt caggacaacg gctatcgtga taaaggcact cagggcgcgc
     5761 agcttggcgg agtcggggtc cgctggaata tctggaccgg cgagacgcac tacgacgcct
     5821 ggccgcacaa agtcagtctc ggcgtcgagt atcaacatac ctttaaggcg attaatcaac
     5881 gtaacggaga gcgcaacaac gcgtttctca ccattggagt gcactggtaa atgcgtaagt
     5941 tcattttcgt attgctgaca ctgcttttgg tcagcccttt ttcctttgcg atgaaaggta
     6001 ttatctggca accacaaaac cgagatagtc aggttaccga tacccagtgg caggggctga
     6061 tgagtcagtt acgtttgcaa ggcttcgata ccctggtttt gcaatggacc cgttacggcg
     6121 atgcattcac ccagccagaa cagcgcgcgt tattgtttaa gcgtgccgca gctgcgcaac
     6181 aggctggcct gaagctgatt gtcgggctga acgccgatcc ggaatttttt atgcaccaga
     6241 aacagtcgtc cgcagcgctg gaaagctatc ttaatcgcct gctggctgcc gatctccagc
     6301 aagccagatt atggagcgcc gcgcctggcg taacgccgga tggctggtac atcagcgcgg
     6361 aaattgacga cctgaactgg cgcagcgaag ctgcccgtca gcctttgcta acatggttaa
     6421 acaacgcgca gcggctgatt agcgatgttt cagcaaaacc ggtttatatc agtagttttt
     6481 tcgccggaaa catgtcgccc gatggctatc gccaactgct ggaacaagtt aaagcaaccg
     6541 gcgttaatgt ctgggtacag gatggcagcg gcgtggataa acttaccgct gaacagcgtg
     6601 aacgttatct acaggccagc gccgattgcc aaagtcccgc ccctgccagc ggcattgttt
     6661 atgaactgtt tgtcgccggc aaaggcaaaa cctttacagc gaaaccgaaa ccggatgcag
     6721 agatcgcctc gctgttagcg aaacgttcct cttgcggtaa agacactctc tatttctctc
     6781 tgcgctattt gcccgtcgcg cacggcattc tcgagtatta aatctcctcc aggtaagtcg
     6841 ggtacgacct ggcttacctc tttcgctctc gtcagaattc catcaagatt aattgctaaa
     6901 aagcgtgcta aagaaagaag atcgttatcg gcgaacgggc cgatcactca tgcaggaagc
     6961 atagatcctt tgagcggagt ttctgatatc tgaggagtgc gaaatgcaat tgagcagcag
     7021 tgaaccttgc gtggtgatcc tgaccgaaaa agaggtagag gtaagcgtca ataaccatgc
     7081 tacgtttacc cttccgaaaa actacctggc cgccttcgcg tgcaacaata acgtcattga
     7141 actctcaacg ttaaatcacg tattaatcac ccacatcaac cgtaacatca tcaacgatta
     7201 tctgttgttt ttaaataaga acttaacctg tgtaaagccc tggtcgcggc tggcaacccc
     7261 ggttattgct tgtcatagcc gtacaccgga agtgttccgg ctagccgcca accacagcaa
     7321 gcagcaaccc agcaaaccct gcgaagcgga gttgacgcgc gtattgcttt ttaccgtatt
     7381 gtctaacttt cttgagcaat cgcggtttat tgccctactg atgtatatct tacgcagcag
     7441 cgtccgcgac agcgtctgcc gcattattca aagcgatatt cagcattact ggaatctgcg
     7501 aattgtcgcc agttcgctat gtttaagccc cagcctgctc aaaaagaaat taaaaaacga
     7561 aaataccagc tatagccaga ttgtcacaga gtgtcgtatg cgttacgccg tacagatgtt
     7621 attgatggat aacaaaaata tcactcaggt ggcgcaatta tgtggctata gcagcacgtc
     7681 atactttatc tctgttttta aggcgtttta cggcctgaca ccgttgaatt atctcgccaa
     7741 acagcgacaa aaagtgatgt ggtgaagggc aaagcggaaa cggataagac gggcataaat
     7801 gaagaagaaa tggcgcgccc tgcaggattc gaacctgcgg cccacgactt agaagttcct
     7861 agaacgacat tttaagtcaa caaattaccg cgccatctct gcgctcacac gtcccactac
     7921 ctcaaaacat gtaaagcctt gcaagccatt gtgaggcctt atgtgtctca gttttgtccc
     7981 tcttttttgt actaaaaaac atagcaattg aggataaacc tcatgctatt ttcgcttata
     8041 tgcctttaaa ggcatggcgc ttaaatagat aaaaacacca caaaagcata aaaaaccaca
     8101 cagtaaaacc gcaacataaa acaataacag ataattaaac caaaaacaga tagcgcattg
     8161 tgataatcat tcaatactaa acaaaatata aacagtggag caatatgtga ttgactcatt
     8221 aagttagata taaaaaatac atattcaatc attaaaacga ttgaatggag aacttttatg
     8281 cgggcgaaac ttctgggaat agttctgaca acccctattg cgatcagctc ttttgcttct
     8341 accgagacta tatcgtttac tcctgacaac ataaatgcgg acattagtct tggaactctg
     8401 agcggaaaaa caaaagagcg cgtttatcta gctgaagaag gaggccgaaa agtcagtcaa
     8461 ctcgactgga aattcaataa cgctgcaatt attaaaggtg caattaattg ggatttgatg
     8521 ccccagatat ctattggggc tgcgggctgg actactctcg gcagccgagg tggcaatatg
     8581 gtcgatcagg actggatgga ttccagtaac cccggaacct ggacagatga aagtaggcac
     8641 cctgatacac aactcaatta tgccaacgaa tttgatctga atatcaaagg ctggctcctc
     8701 aacgaaccca attaccgcct gggactcatg gccggatatc aggaaagccg ttatagcttt
     8761 acagccagag gtggttccta tatctacagt tctgaggagg gattcagaga tgatatcggc
     8821 tccttcccga atggagaaag agcaatcggc tacaaacaac gttttaaaat gccctacatt
     8881 ggcttgactg gaagttatcg ttatgaagat tttgagctag gtggcacatt taaatacagc
     8941 ggctgggtgg aagcatctga taacgatgag cactatgacc caggaaaaag aatcacttat
     9001 cgcagtaaag tcaaagacca aaattactat tctgttgcag tcaatgcagg ttattacgta
     9061 acacctaacg caaaagttta tgttgaaggc gcatggaatc gggttacgaa taaaaaagga
     9121 aatacttcac tttatgatca caatgataac acttcagact acagcaaaaa tggtgcaggc
     9181 atagaaaact ataacttcat cactactgct ggtcttaagt acacctttta acgaagttaa
     9241 ccagattttc tccccggtgg tattgtataa ccccggggaa tttgtttaca gttacaagta
     9301 ggcaatatcc actaattttg taagcattta acctatgtca taacgtgtaa tctcttaccg
     9361 caacacaaga cctcaatata ttcaaatctt atctttattc cgatattcag agacacaatg
     9421 cgaaaaataa ttactcattt caaagttgtt ttaacgttac ttctaccagt aaccgtatct
     9481 gcccagcaga tacagtggca atcctgtatg gccagtcaat tcaaccactg gtttggtgag
     9541 gaaaaaccgt ctcctgactt actatgtggt tatttgtctg ttccattaaa atatacagac
     9601 acaggcggag atgcttctta tgaaaaaaaa tcacaagtca aactagcgtt gacaaaattg
     9661 ccggcaaaaa gcaagcataa aggaagtatc ctgataataa gtggtggtcc cgggttacca
     9721 ggcataaatc cttatattaa ctttgactgg ccagtcacaa atcttcgtga gtcatgggat
     9781 attattggat ttgatcctcg aggcgtcgga cagtccactc cgacaataaa ctgccggcaa
     9841 tcagatacag agactcagga aaacataacc gaaaagcaac aagtattaaa taaaattaat
     9901 gcctgtatcc ataataccgg agccgaagtc attcgccata tcggctctaa cgaggctgta
     9961 tacgatattg atcgtattag gcaagccttg ggggataaac aactgacagc cgtggcgtat
    10021 tcgtatggaa ctcaaattgc agccttatat gcagaacgtt ttccctacaa cgtaagatct
    10081 atcgttcttg atggagtcgt cgatatcgat gacctggagg acaacttcac atggcaactc
    10141 aaacaggcac agagttatca ggaaacgttt gatcgctttg catcctggtg tgcgcgtaca
    10201 aaaagttgcc cgctttcttc agacagagat aaggcaataa ctcagttcca tgagctatta
    10261 tcaaaattac atcacaaacc tttattagac agtaagggag aaaatatatc ttcagatgaa
    10321 ctcatatcat taacaacaga ccttctgcta tggcgttcat catggccaac ccttgcaact
    10381 gccatacgcc agttctctca ggggattgtc agtaatgaaa ttgaaactgc gctcagtgct
    10441 ccgatagcct cagaagagtc aagcgatgct tcgggggtaa tcctctgtgt agatcagggg
    10501 gatgagcaat taacaccaga agagcgaaaa tcccgaaaag acgctcttgc gaatgccttc
    10561 ccggctatta actttgacaa tggacgttcc gattcacctg atttttgtga attatggcca
    10621 atacatagcg acctgaacaa aactcgcctg aaaaatactg ttctgccctc tggtttactg
    10681 tttgtagcac acaaatacga cccaacaacg ccctggatta atgcccgtaa gatggcagag
    10741 aaattttcca gcccgttact aacaataaat ggtgatgggc atacattagc tctcaccgga
    10801 gttaatttat gtgtagataa agcagttgta cattacctga tcactccaca aaaaatagaa
    10861 aatatatact gcccaggaaa ttctgaagca gaaatacaat aatttcaaaa tattccgcta
    10921 tttgc
//
LOCUS       DAZFXT010000149        10907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.149, whole genome shotgun sequence.
ACCESSION   DAZFXT010000149 DAZFXT010000000
VERSION     DAZFXT010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.149"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            63..824
                     /gene="adiY"
                     /locus_tag="ACHKW0_004168"
     CDS             63..824
                     /gene="adiY"
                     /locus_tag="ACHKW0_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HFO7476908.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            961..2298
                     /gene="adiC"
                     /locus_tag="ACHKW0_004169"
     CDS             961..2298
                     /gene="adiC"
                     /locus_tag="ACHKW0_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HFO7476909.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2402..4045
                     /gene="eptA"
                     /locus_tag="ACHKW0_004170"
     CDS             2402..4045
                     /gene="eptA"
                     /locus_tag="ACHKW0_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HFO7476910.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4042..4710
                     /gene="basR"
                     /locus_tag="ACHKW0_004171"
     CDS             4042..4710
                     /gene="basR"
                     /locus_tag="ACHKW0_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HFO7476911.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHKW0_004172"
     CDS             4711..5811
                     /gene="pmrB"
                     /locus_tag="ACHKW0_004172"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HFO7476912.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDDGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHKW0_004173"
     CDS             complement(5787..5876)
                     /gene="pmrR"
                     /locus_tag="ACHKW0_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HFO7476913.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHKW0_004174"
     CDS             complement(5988..7490)
                     /gene="proP"
                     /locus_tag="ACHKW0_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HFO7476914.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHKW0_004175"
     CDS             complement(7754..8632)
                     /gene="rdcB"
                     /locus_tag="ACHKW0_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HFO7476915.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRKHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGVFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHKW0_004176"
     CDS             complement(8629..10857)
                     /gene="crfC"
                     /locus_tag="ACHKW0_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HFO7476916.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEMRTRCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSAVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2726 a         2849 c         2692 g         2640 t
ORIGIN      
        1 tttcccggta tcgctttgct ttagcgagaa ctggtctttt attcgcactc aggagtacat
       61 gtatgaggat ttgcagcgac caaccttgta ttgttttatt gactgaaaaa gatgtctgga
      121 taagggtgaa tgggaaagaa cctattagcc ttaaagctaa ccatatggcg ttattaaatt
      181 gtgaaaataa tattatcgac gtctcctctc ttaacaacac tttggttgct catattagtc
      241 acgacatcat caaagattac ctccggtttc tgaataaaga tctctcgcaa ataccagtat
      301 ggcaacgtag cgctacgccc atactcaccc tgccatgcct gacgccagac gtctttcgcg
      361 ttgccgcgca acacagcatg atgcccgcag aaactgagtc agaaaaggaa cgaacacgcg
      421 cattattatt cacggtgcta tcccgttttc tcgacagtaa aaaattcctt tcattaatga
      481 tgtatatgtt acgtaattgt gtaagtgaca gcgtttatca aattattgaa agcgatattc
      541 acaaagactg gaatcttagt atggtagcca gttgtttatg tcttagccca agtctgttaa
      601 agaaaaagtt gaaaagcgaa aacaccagtt atagccaaat aatcaccacc tgccgcatgc
      661 gttatgccgt aaatgaatta atgatggacg gtaaaaatat ctctcaggta tcacagtcct
      721 gcggctacaa cagtacgtcg tactttattt ctgtctttaa agacttctac ggtatgacgc
      781 cgctgcatta tgttagtcag cacagagaac gcactgtcgc ctgattttta accttaacga
      841 agagctatat taataacggc atcagcgata acccggtcga taataattca actatcgaat
      901 gcaggcgtat gatatgacgt aatttattgt cacgaagctc gccttcgcag gagtttaatt
      961 atgtcttcgg atgctgatgc tcacaaagtg ggcttaatcc ccgtcaccct gatggtgtcg
     1021 gggaatatta tggggtcagg tgtttttctg ttacctgcaa acctggcctc gactggcggg
     1081 attgccattt atggatggtt ggtgacgatt atcggtgcgc tggggctctc gatggtatac
     1141 gccaaaatgt cgttcctcga tccaagtcct ggtggttctt acgcttacgc ccgccgctgc
     1201 tttggcccgt ttctcggtta tcaaaccaac gtcctctact ggctggcctg ctggatcggc
     1261 aatatcgcca tggtggtcat tggcgtagga tatttaagtt acttcttccc gattctgaaa
     1321 gatccattgg tattaaccat cacctgcgtc gtggtgctgt ggatcttcgt cctgctgaac
     1381 attgtcggtc cgaaaatgat cacccgtgtg caggcagttg ccaccgtgct ggcgctgatc
     1441 cccatcgtcg ggattgccgt atttggctgg ttctggttcc gtggtgaaac ctatatggcg
     1501 gcatggaacg tcagcggcct gggcaccttc ggtgcaattc aaagtaccct taacgttacg
     1561 ctgtggtcgt tcatcggtgt ggaaagtgcc tccgttgccg caggtgtggt gaaaaacccg
     1621 aaacgcaatg tccctatcgc caccattggt ggggtattga ttgccgccgt ttgctatgta
     1681 ctttctacca ccgcgattat ggggatgatc cctaatgccg cactgcgcgt ttctgcttcg
     1741 ccattcggtg atgccgcacg gatggcgttg ggtgacaccg ccggggccat tgtttccttc
     1801 tgcgcagctg cgggttgctt aggttcactg ggcggctgga cgttgctggc gggtcaaacg
     1861 gcgaaagccg ctgccgatga cggactgttc ccaccgattt ttgcccgtgt aaataaagcg
     1921 ggtacgccag tggcggggct gattatcgtc ggtattttga tgaccatatt ccagctcagc
     1981 agcatttcac caaacgcgac caaagagttc ggtctggttt cttccgtctc ggtcatcttt
     2041 acactggtgc catatcttta cacctgtgcg gcgttactgc tgctcggaca cggtcacttt
     2101 ggtaaagcac gcccggcata tctggcagtt actaccattg ccttcctcta ctgcatctgg
     2161 gccgtggtgg ggtccggagc gaaagaggtt atgtggtcat ttgtcaccct gatggtcatc
     2221 accgccatgt atgcgctgaa ttacaaccgg ctacataaaa acccgtatcc cttagatgca
     2281 ccaataagca aagattaatt ccccttaatc cagcaaacat aaaagccaac cttaagaact
     2341 taaggttggc ttaattttgc tttgcgagca tatgcgcact ttgttcgatg gaaacaccgt
     2401 gatgttgaag cgcctactaa aaagaccctc tttgaattta ctcgcctggc tattgttggc
     2461 cgctttttat atctctatct gcctgaatat tgcctttttt aaacaggtgt tgcaggcgct
     2521 gccgctggat tcgctgcata acgtactggt tttcttgtcg atgccggtcg tcgctttcag
     2581 cgtgattaat attgtcctga cactaagctc tttcttatgg cttaatcgac cactggcctg
     2641 cctgtttatt ctggttggcg cggctgcaca atatttcata atgacttacg gcatcgtcat
     2701 cgaccgctcg atgattgcca atattattga taccactccg gcagaaagtt atgcgctgat
     2761 gacaccgcaa atgttattaa cgctgggatt cagcggcgtg cttgctgcgc tgattgcctg
     2821 ctggataaaa atcaaacctg ccacctcgcg tctgcgcagt gttcttttcc gtggagccaa
     2881 tattctggtt tctgtactac tgattttgct ggtcgccgca ctgttttata aagactacgc
     2941 ctcgttgttc cgcaataaca aagagctggt gaaatcctta agcccctcta acagcattgt
     3001 tgccagctgg tcatggtact cccatcagcg actggcaaat ctaccgctgg tgcgaattgg
     3061 tgaagacgcc caccgcaacc cgttaatgca gaacgaaaaa cgtaaaaatt tgaccatcct
     3121 gattgtcggc gaaacctcgc gggcggagaa cttctccctc aacggctacc cgcgtgaaac
     3181 taacccgcgg ctggcgaaag ataacgtggt ctatttccct aataccgcat cttgcggcac
     3241 ggcaacggca gtttcagtac cgtgcatgtt ctcggatatg ccgcgtgagc attacaaaga
     3301 agagctggca cagcaccagg aaggcgtgct ggatatcatt cagcgagcgg gcatcaacgt
     3361 gctgtggaat gacaacgatg gcggctgtaa aggtgcctgc gaccgcgtgc ctcaccagaa
     3421 cgtcaccgcg ctgaatctgc ctggtcagtg catcaacggc gaatgctatg acgaagtgct
     3481 gttccacggg ctggaagagt acatcaataa cctgcagggt gatggcgtga ttgtcttaca
     3541 caccatcggc agccacggtc cgacctatta caaccgctat ccgccgcagt tcaggaaatt
     3601 taccccaacc tgcgacacca atgagatcca gacctgtagc aaagagcaac tggtgaacac
     3661 ttacgacaac acgctggttt acgtcgacta tattgttgat aaagcgatta atctgctgaa
     3721 agaacatcag gataaattta ccaccagcct ggtttatctt tctgaccacg gtgaatcgtt
     3781 aggtgaaaat ggcatctatc tgcacggtct gccttatgcc atcgccccgg atagccaaaa
     3841 acaggtgccg atgctgctgt ggctgtcgga ggattatcaa aaacggtatc aggttgacca
     3901 gaactgcctg caaaaacagg cgcaaacgca acactattca caagacaatt tattctccac
     3961 gctattggga ttaactggcg ttgagacgaa gtattaccag gctgcggatg atattctgca
     4021 aacttgcagg agagtgagtg aatgaaaatt ctgattgttg aagacgatac gctgttattg
     4081 cagggattga ttctggcggc gcaaactgaa ggctacgcgt gcgatggcgt gacaaccgcg
     4141 cggatggcgg aacaaagcct tgaggccggt cattacagcc tggtggtact ggatttaggg
     4201 ttacccgacg aagatggact gcattttctc gcccgtatcc ggcagaaaaa atacaccctg
     4261 ccagtactga tcctcaccgc tcgcgatacg ctgaccgaca aaatcgctgg gctggatgtc
     4321 ggtgccgatg actatctggt gaagcctttt gcgctggaag agttacacgc ccgtatccgc
     4381 gccctgctac gacgccataa taatcagggc gaaagtgagc tgattgttgg caatctgacg
     4441 ctgaatatgg gtcgccgtca ggtatggatg ggcggtgaag agttgattct gacgcccaaa
     4501 gaatatgctc tgctgtcacg gttaatgctc aaagcaggca gtccggtgca tcgggaaatt
     4561 ctctacaacg acatctataa ctgggacaat gaaccctcga ccaacaccct ggaagtgcat
     4621 atccacaatc tgcgcgacaa agtgggcaaa gcccgtatcc gcaccgtgcg cggctttggc
     4681 tatatgctgg tcgcgaatga ggaaaactaa ttgaatctga tgcattttct gcgccgacca
     4741 atatcgctgc gccaacggct gatattgacc atcggggcca ttttgttggt gtttgagctg
     4801 atcagcgtct tctggctatg gcatgaaagt accgagcaga ttcagctgtt tgagcaggcg
     4861 ctacgcgaca atcgcaacaa cgatcgccac atcatgcgtg agatccgcga ggcggtcgcc
     4921 agcctgattg tccccggcgt ctttatggtc agcctgacgc tatttatctg ctatcaggcg
     4981 gtgcgccgca tcacccgccc gctggcggag ctgcaaaaag agctggaagc gcgcactgcc
     5041 gacaacttaa cgcccattgc cattcacagc gccaccctcg aaatcgaagc ggtggtttcg
     5101 gcgttaaacg atctggtcag tcgcctgacc agcacgctgg ataacgaaag gttgtttacc
     5161 gctgacgtcg cgcacgaact gcgaacgcca ctggcggggg tgcgtttgca tctggaactg
     5221 ctggcgaaaa cgcatcacat tgatgtagca ccgttagtgg cacgacttga tcagatgatg
     5281 gagagcgtct cccagctgct gcaactggcg cgtgccggac agtcattttc ttccggtaat
     5341 tatcaacatg taaaactgct ggaagatgtc attctcccct cgtatgacga actcagtacc
     5401 atgctcgacc agcgacagca aaccctgcta ttgccagaga gcgccgccga catcaccgtt
     5461 cagggtgatg caaccctgct gcggatgtta ttgcgaaacc tggtagaaaa cgcccatcgt
     5521 tacagcccgc aaggcagcaa cattatgatt aagctgcaag aagatgacgg agcggtcatg
     5581 gcagttgaag atgaaggacc aggtattgat gagagtaaat gcggggagtt gagtaaagcg
     5641 tttgtacgta tggacagccg ttatggcggg attggtctgg ggttaagtat tgtcagccgc
     5701 attacacagt tgcatcacgg gcagtttttc ctgcaaaacc ggcaagagac ttccggcacg
     5761 cgggcctggg tacggctgaa gaaagatcag aacgtggcaa accagatata aagaaagctg
     5821 ctgaccacca gcacgctgaa cacggtagtt aaactttcat aaacacggtt tttcattgca
     5881 ctctcctccg ttaacctgga ggagagtatg cgcgtcagag attaagtgaa ccttaagagt
     5941 tcaattggcc tgataagaca gcgtcacatc aggccatccg tttcagctta ttcatcaatt
     6001 cgcggatgtt gctgcaccag gcgggtacgt ttcgcctgca aatcggcaat ctcgtggtca
     6061 atattatcga ttttctgctc gatattatcg taatgctcga cgagaatttc cttcgcttcc
     6121 tgtatatctg acgccgccgg tgtcgcacct ttcaacggac gatttgccgt ctctttcatg
     6181 gttacgccgg tgattaaacc aatcaccgcc actaccatca ggtaataggc aggcatcatc
     6241 agattctgcg agctttcgac cagccaggcc gccagtgttg gcgtcagacc ggcaaccagc
     6301 accgaaatat taaatgccgc cgccagcgcg ctgtaacgga tatgcgtcgg gaacatcgct
     6361 ggcaaggtag aagccataac gcccgtaaag cagttaagga tcaccgccag catcagtaac
     6421 ccggcaaaaa tcaggccgat gacgttactg ttaatcagaa taaacgccgg gatcgccaac
     6481 acaaacaggg caacactacc aagtagcaca aacggacgac ggccaaaacg gtcactcagc
     6541 aagcccatca ccggctggac aaacagcata ccgatcataa tggcgataat aatcagcacc
     6601 ccgtggtctt cggagtaatg caggttatgc gacaaataac tcggcatata ggtcagcaac
     6661 atgtagtaag tcacgttggt ggcaattacc agaccaatac atgtcaacag gctgcgccag
     6721 tgtttggtgg caatctcttt aaacgagact ttcgggccat cctgcaaacc ttcgcggtcg
     6781 ccctgttcca gtttatcgac atgctgctgg aacgccggag tttcttccag cgcatggcgc
     6841 aggtaaagcc cgataatccc taacggcaga gcaataaaga acggaatacg ccagccccag
     6901 tcgaggaagt tcgcttcgcc gacaatggtc gaaattaaca ccactacgcc agcacccagc
     6961 acaaacccgg caatagaacc gaagtccagc cagctgccca taaagccacg tttacggtca
     7021 ggtgagtatt ccgcaacaaa tatcgacgcc ccggtatatt caccgccgac cgagaaacct
     7081 tgtgccatct tacagatcaa cagcagaatc ggtgcccaaa taccaatcgt gtcgtaggac
     7141 ggtattaagc caatacagaa cgtactgatc gacataatca caatagtgat agcgaggatc
     7201 ttctggcgac catatttatc gcccaacata ccaaagaaga gcccgccaag cggtcgaatc
     7261 agaaagggaa cggagaacgt ggcaagtgca gcaaccatct gcacgctggg gtcagccccc
     7321 gggaaaaaaa ctttacctaa tgcgtaagca acaaaaccat aaacaccaaa atcaaaccat
     7381 tccattgcat tacccagtga tgctgcggta atggctttac gcagtttacc gtcatcaata
     7441 atggtgacat cacgaagggt aatcggtttt acttttttcc ttttcagcat agctttcctc
     7501 gcagagttgg cccatttccg caaacgccag aaaaatcagc gcgacggccg caaaagggga
     7561 gcgggaattg caggatgcaa tctctgtaac aagcgtagcg ggtttgctta caccctccgg
     7621 ttaaggaatg tacaatcaaa ccagttgacg cctgaattgg gcagttaatg acctttttac
     7681 cctaccagtg tttaaatttg tgatcaactt cacacatgga tttcgtttag gactcattga
     7741 tgtaactgat tatttacgcc aaatttacag agaagatttc ctcgaccatt gcccccgcca
     7801 gacgctgcgc tgaacagagt cgtggcatcc ccagcgcgac agtttgccag cgttgggtca
     7861 ctgaccagca aacgggttgt ttatctgccg tggtccactt tcccagccaa cttaaaaaat
     7921 cagcatgatc cccgcccgtc ggaacggtcg aagaggcaaa ccactgatgg taatagtgac
     7981 gcgtggtcgg ggccgcattg atactctctg ccagatgttg tgtcgctttc acgcgcaaat
     8041 cacccagcaa caacgccacc gcttgcgggc ttcccagacg acaggcatcg ccaaataccc
     8101 cccaacgcaa ctcttcgaga acgataaaac agcgcccagg caacgaccag gcgttatacg
     8161 ccccagcacg ccagcgggta aaaatttgct cgctctgttc acccgcctgc accagtaggc
     8221 ctcgttgcgt taatgccttt tccttccagc gacaacgggc tgaaaaccac tcatacaagg
     8281 cggcaatttg ctgggggagc tccgggaaag cagagtgctt gcggtgatcc attttttgca
     8341 gcagcgccag atgctgcgcg atctgcgtca gcgccagatg accgggatcc agcatcgacg
     8401 ccagtttttc caccattgtc agtcgcaggc taagcggcgc atttaacatg ccgccgttcg
     8461 tccatgtctg aagctgcaca cgcgaaagaa gttcctgcat cagtcgttca cgaaactgct
     8521 gctgatgggc ttgtggaagg cgggcatcat cgccctgcgc cagatccact aaaaagcggg
     8581 gataaaccga ctccagcacg cgaccggggc cgtccagtaa cgtcttggtc aatatcgttc
     8641 tgccgtgaaa agtgtttgaa tatcatcgcg taacagctgg gcgtcggtgt aaatccagcc
     8701 gtgagtcatc acagtctgct gcaattgctg ctgcatcagc ctgaccaccg attcattttg
     8761 ttgacgcaga gccaggcttt cgcgtaaacg cgtctgtaat tccgtcaaac atgaagcgaa
     8821 ctcagcgaaa aaagtattca tgcctgccgt aacagattca tcgacctgct ctgccagaac
     8881 tttagccatt tgttggcaat aaagatcgac ttctgcgctt aatgctcgtt gcaacacact
     8941 gtaatcaacc gtttctgtcg gggatttctc atttccccgt ccccagtcgg gctgattcaa
     9001 ccagcgcgaa aaagtctcac gcacaacgcc taaacgcgtg ctctgctcgt ccgttgcatc
     9061 ctggcgcgaa atgactgcac tgaacagctg gcgagtgttg aagtggggaa ctacgccgtg
     9121 aaaaacagga aaatgaaacc caggacgaaa ccctgactcg ctcaattcca ttttgacttg
     9181 ttgctcaatg gggcgaataa catcggttaa cacccggcaa agggtggatt ccagctcggc
     9241 aaaacgcagc gtaaagtcgc gactgatggt gttctgcgcc gtctgtaaca gtgtctcaca
     9301 gcgggtacgc atctcgctta acggctccga atcatcctga aacaaggcgg ctaactgcgc
     9361 attcagcgca tcttgttgtt gacgcagaaa gtggttggcg gaggtcaggg ccagctcgat
     9421 ttcatgttta atctcgccgc tcacctgcgc ctgattgagt tgcaatagct gcaaactttc
     9481 ttcgacctga tggatatttt gccgcaattg ttcacaagcg acgtttaacc cgtgcgcacg
     9541 aaaatccagg tattcccgcg cctgctgcgc gtaattcaac agtttatgcg cagcagatcg
     9601 caaagcatac aacgaggcgt tagcgtaagc ggcatgaagc aacgcctgaa ttggctgggc
     9661 gaacagcgaa tcttcccaca actgatcggc agcatgacga atatgttcga ggtccgccag
     9721 atcggcatga cgccagcgcc tgccgagcgc ggcatgggca aaatcttcca cccagcgttg
     9781 ttgctctggc gctggtaact taccgttgtt ggctaactca tggcgcgccc gattcgccag
     9841 gtagccccac atcgacgaca ccggaaatat ctgctgtggc gtaatacagc ctttcatcag
     9901 cgtcccggaa atcagtgccc gcacctggtc ggcgtcgtca ctgttacgat cctgttgatc
     9961 gaacttattg accagcacat acagcggcac cgattgcccc accgccaaaa tcgcctcacg
    10021 gacctcttca tcggagatcg atttcagttg cgtataatcc agcaccgcca gtaccgccga
    10081 ggcgcgtgcc agctgctggt taagcatttt ttgcagatgc ggttgcccgg cttcatttgg
    10141 cccgggggta tccagtaacg tcaactgacc gggataactc tccagccccg ccagatggac
    10201 aaactccact tcaatcacgg gaatatgctc aatggcggcg taagcagaaa aaggaaaatc
    10261 gacgtccagc gccttcgcca gtcgcactaa atcattcaaa cttttcagac aatgaaaaat
    10321 aggctgggca cccagataat atttttcgaa agcgacgcca ttttcgatcc gctgcataag
    10381 cgcacgcata tctttatcta tttccagcac atcggtcaga tgcttaatat cgcaatcacg
    10441 caggcgctgt tgtaattgtt gaattaaaca atcgattggc gcgacatgtg aaaaatgcag
    10501 taccggttcc ttttgcccgg gcgtatggcg aataagcgtc ggcagcgcag tcattgggcg
    10561 attacgatta ggcagaacct ccgtaccaac aatggcatta atggtggttg atttccctgc
    10621 tttcatggta ccgacaattg ccagcaccat ttccagtcgg gaaattttac gcaactcatt
    10681 attcagcatc gcgtgacgtt cggcgatatt aggctgactc cagggtaaag ccagttgtgg
    10741 cgcgtcgtct ccgggtacag agagaggcat tttttccagt aactgcaact gttggcgaga
    10801 aagctgtaac aggcgttcag cctcctgact taactcatac agggtctgtg tgtacataga
    10861 aaattcttcc ttaaagcaaa ttttgttact tttatttagc cagattg
//
LOCUS       DAZFXT010000150        10865 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.150, whole genome shotgun sequence.
ACCESSION   DAZFXT010000150 DAZFXT010000000
VERSION     DAZFXT010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.150"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            32..601
                     /gene="nleF"
                     /locus_tag="ACHKW0_004177"
     CDS             32..601
                     /gene="nleF"
                     /locus_tag="ACHKW0_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector caspase inhibitor NleF"
                     /protein_id="HFO7476917.1"
                     /translation="MLPTSGSSANLYSWMYVSGRGNPSTPESVSELNHNHFLTPELQD
                     KLDAMISIYSNARNSNELEEIYQELSAFVSGLMDKRNSVFEVRNENTDEVVGALREGM
                     TIDDRDGYIRELFFLYSLKVKIEESRQGKEDSKCKVYGLLCPHHSSELYGDLRAMKCL
                     VEGCSDDFDPFDIIRVPDLTYNKGALQCG"
     gene            complement(1017..1105)
                     /locus_tag="ACHKW0_004178"
                     /pseudo
     CDS             complement(1017..1105)
                     /locus_tag="ACHKW0_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            complement(1946..2476)
                     /gene="yodB"
                     /locus_tag="ACHKW0_004179"
     CDS             complement(1946..2476)
                     /gene="yodB"
                     /locus_tag="ACHKW0_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HFO7476918.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(2819..3469)
                     /gene="zinT"
                     /locus_tag="ACHKW0_004180"
     CDS             complement(2819..3469)
                     /gene="zinT"
                     /locus_tag="ACHKW0_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HFO7476919.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(3726..4361)
                     /gene="msrQ"
                     /locus_tag="ACHKW0_004181"
     CDS             complement(3726..4361)
                     /gene="msrQ"
                     /locus_tag="ACHKW0_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HFO7476920.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(4362..5366)
                     /gene="msrP"
                     /locus_tag="ACHKW0_004182"
     CDS             complement(4362..5366)
                     /gene="msrP"
                     /locus_tag="ACHKW0_004182"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016667 - oxidoreductase activity, acting
                     on a sulfur group of donors [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HFO7476921.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(5475..5888)
                     /gene="hiuH"
                     /locus_tag="ACHKW0_004183"
     CDS             complement(5475..5888)
                     /gene="hiuH"
                     /locus_tag="ACHKW0_004183"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HFO7476922.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            6021..6692
                     /gene="hprR"
                     /locus_tag="ACHKW0_004184"
     CDS             6021..6692
                     /gene="hprR"
                     /locus_tag="ACHKW0_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HFO7476923.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            6692..8050
                     /gene="hprS"
                     /locus_tag="ACHKW0_004185"
     CDS             6692..8050
                     /gene="hprS"
                     /locus_tag="ACHKW0_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HFO7476924.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDSINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEVDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHQALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSHIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(8157..9008)
                     /gene="hchA"
                     /locus_tag="ACHKW0_004186"
     CDS             complement(8157..9008)
                     /gene="hchA"
                     /locus_tag="ACHKW0_004186"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036524 - protein deglycase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase III HchA"
                     /protein_id="HFO7476925.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAG"
     gene            complement(9601..10674)
                     /locus_tag="ACHKW0_004187"
     CDS             complement(9601..10674)
                     /locus_tag="ACHKW0_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7476926.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEIYNKDGNKVDFYGKMVGERIW
                     SNTDDNNSENEDTSYARFGVKGETQITSELTGFGQFEYNLDASKPEGSNQEKTRLTFA
                     GLKYNELGSFDYGRNYGVAYDAAAYTDMLVEWGGDSWASADNFMNGRTNGVATYRNSD
                     FFGLVDGLNFAVQYQGKNSNRGVTKQNGDGYALSVDYNIEGFGFVGAYSKSDRTNEQA
                     GDGYGDNAEVWSLAAKYDANNIYAAMMYGETRNMTVLANDHFANKTQNFEAVVQYQFD
                     FGLRPSLGYVYSKGKDLYARNGHKGVDADRVNYIEVGTWYYFNKNMNVYTAYKFNLLD
                     KDDAAITDAATDDQFAVGIVYQF"
BASE COUNT         3067 a         2178 c         2463 g         3157 t
ORIGIN      
        1 tataatattt atttgttaag ggggttttga tatgttacca acaagtggct cttcagcaaa
       61 tctttattca tggatgtatg tatcaggaag aggtaaccct tcgactccgg aatcagtaag
      121 tgaacttaat cataatcatt ttcttactcc tgaattacaa gataaacttg atgctatgat
      181 ctctatatac tcaaatgcca gaaatagtaa tgagcttgag gaaatttatc aagagctaag
      241 tgcttttgta agtgggctga tggataagag aaatagtgta tttgaggtga gaaatgaaaa
      301 tactgatgag gttgtcggag cactgaggga ggggatgacg atagatgaca gggatggtta
      361 tatcagggag cttttttttc tgtattcatt gaaagtaaaa attgaggaaa gtagacaagg
      421 taaagaagat tctaaatgta aagtttatgg tctgttatgt ccgcatcact cttcagagct
      481 atatggtgat ctacgagcaa tgaaatgtct tgtggaagga tgcagtgatg attttgatcc
      541 ttttgatatt attagggtgc cggatcttac ttacaacaaa ggagctttac aatgtggatg
      601 attagaggga ggtcctgtga gcctgcttat cattttgtgt aaacgctttt tttaaaagtg
      661 accatcaagg tggctaccaa gctcgattat aaagtagcat tgtccatttc tgtgatgtgg
      721 attgccagcc aatcactttg tttgctgact tgtagtcggg gatatcgggg gcaccttgca
      781 tttcctgacg gcgtgctata cgacacctca ctgtacacac gtagttacaa atttacccca
      841 gcgcaatggt ctcaggagag gggcgattaa tttcatcaga tattcttcgt cagaaggtgg
      901 tgattatcca tgatatttgt gtatcgactt tttataagag gtttctggtc gggattatat
      961 aaaactgcgg acacgtcgta tgcaagaagg cgctacggtt ttctgataaa cctttcgcca
     1021 gcatcatctg cttcagccca gtaaaaagag acgcgaaaaa tgcacaacag gcaccacacg
     1081 tcatgcatgg attaggattg ctcataaatt cactacttga gtatcagtaa tgttaaggga
     1141 tatcaggaaa tattttatga gaaagaaaaa cagcaaactg gtcaaaaata actcaaagaa
     1201 ggcttggaat attcttattg aattcaacgt agtttattga tttttcgtgt atgtttttaa
     1261 gacatttatt ccaagaaaaa tttataacct tttgattttg cgattcggta tcatcggtct
     1321 cgaaaaccgg agtaggggca actctaccgg gggttcaaat ccccctctct ccgccacttt
     1381 atcaatgact tatctcccga cttcccgcct tgcttttcct aaacagaaca atcgtagaat
     1441 attcttgaag ggttagatcg tcactgtttt ctgttcgata ctgtgacatt cagcacttga
     1501 ttcgctatgg atctgacagg aagctttcga gcgaaaatgt gcagttattc agtcgttttc
     1561 ttatcgctca ccattattct tttagacatt catcctatga aactgccgca aagttggtgg
     1621 tgggaactga agttgcgtag agaaggggtc aataattgag tcaaaaaagt aacgcggccg
     1681 tcagattaaa gccgcgggac aaagtccata atttccaaga gttgttgaat ttcccagctt
     1741 ttttgaaagc ctttgtatca tactaacaag taaaaaaggt ttaaatacca aaattaacct
     1801 gtatttttga taagtcttac cattgtacga tttacacagt ttctgctcct ccttgttgat
     1861 agtggaaagg gtgcattgtg cacttgcgta aacatttatg acgcgttgat gtccatggtt
     1921 cacatttcct tgtcagggga tcgacttatt tgcggcgagg catcattcgt aacagagtat
     1981 tatctttctg aatgtagtga tgaaagagtg ctgcgccagc gtgagctgcg attaaaaagt
     2041 aacctatatt tgcccaggtt tcgtgaatat tttttatcag tgctttaatt tcgctgtttg
     2101 gggtaacaaa gggagacaca ttgaaaccaa ggaaactcca cgattttcca ctgtaagcca
     2161 tcaaagcaat ccccagcaga ggaagtgcaa ggaaggttat ataaagcatg atgtgcatta
     2221 gcgaagccgc tttcatttgc caggcaggtg gcggtggcac aatagaagga tcactataac
     2281 ggtgttttat aatcaggcgt gaaaacatta acacccaaac gaatataccc gcattgtaat
     2341 gtgtttctcg catcagcaga taagtactac tacctttagg aaaccagcca cggagttcca
     2401 tcgcggcata ggttattgca acgaaaagca gcgttatcca atgtaaataa atttgagttt
     2461 ttgagaatcg gttcattttt gtagctcctg taatttttac agtcaagcta tcccacgaac
     2521 cttaaaaaat ccttatacag atgtggtttt aagaatttgt tatttaaaaa attgatagtg
     2581 cacattgtaa ccagccaata taagcaatgg cgtgagcaca ggtatctttc tctgttggcc
     2641 gtattgtgta tggaatccgt tattggttaa ctcaatgcgg gttagcttat taatatacag
     2701 acacgtcata gtgatgagcg tgtctgtagc tagcttgcgt tcagtggcgt ttgtggatgg
     2761 accagtggca gttatgatga cttactaaag cggcatcgag gcgttatcat gagaatactc
     2821 aatgagacat catttcctcg accacttctt cgctactcaa ctgatatgga taatacgttg
     2881 gccagttttc catttcattt aataaagatt gctgtgaatc attacccata aaaatgtgaa
     2941 aatgagatga ttttcgtggt gcaattatat gatcgctaaa ttgtatatat ttaggggctt
     3001 tgctttcagg atctttacat tcgaataagt aacgaacgcc tttcttgcct gatttatagg
     3061 tgagtatttt gtatccatcg taatcatatt tacaggatgt tgtttcatta tttctatgga
     3121 attcaacaat gccgtcctca atgccaatca tctcgatatc tgttgcataa cctttgtgat
     3181 agtaatcttt aatttcagca aatgttttag ttttatctgc atccgctttc ttctgaaaga
     3241 cggggtcaag tttgccactt tgcagtaaag gataaacgga ttgccagact ccatcccagt
     3301 cactgagcgt tcggttttgt acattggcat catcaaaaac accattagca gctttttgtt
     3361 cgacctctgt taagggtttg ccgtgtgagt gatgaccatg cgaaaaggca ggagcgctaa
     3421 caataaagac acctaaagca acagccagtt tgtgaagacg aatcgccaaa acagtttcct
     3481 ccagagtcat tgtatatgat gtgtaatgtt atattgtaac atatggcaaa atgcaacaac
     3541 tcttaggaga tgagagcata gttttctcag gtgtaattat ctatcttatt gaaataaaat
     3601 gaattatatt gataatttga ggttgatgac aaacgaaaca taacctgatg tgatctgcgt
     3661 aggccggata gggcgctcgc gccgcatccg gcagggataa cgcgtacttt gtcggcaata
     3721 tgaagttata cagataattt gttgtgaacc tgcttgcgta accggttaaa aagggaacgc
     3781 aacttcttat accgtaaggc taaaagcagt acagccagcc cagcgtagat gagcggttgc
     3841 ggtgaaataa tcttcactga ccacagataa tgtattgggg ccaggatcgc gacaagatag
     3901 acgaaattat gcaactgttg ccagtgcttg ccgagttttc gctgcatcgc ctgggttgaa
     3961 gtaaacgcta aagcaagcaa aataacccag ctgataattc ctaacgttaa ataaggccgg
     4021 gtaattaact ccttgcctaa taacgccaga ttattcacgc ccagctccag caatgcgtaa
     4081 ctggttaaat gcagtgtcgc ccaggcaaag caccataagc ctaacaggcg gcgagtgcgt
     4141 atcaataacg gctgttttgc gtagcgtgcc agaggtgtga ttaacaaggt cgccagcaaa
     4201 aatttcagtg cagtgcgacc ggtaaaatgc tgaatatctt tcaccggatc ggcacccagt
     4261 ccaccgtgat tgatcgccca gaccagccag agaaacggca acaatccggc aagatgcagg
     4321 ctaactttca gccatgtcac ctgttttgct gtcagacgca ttcagaaatt ctcccgcaaa
     4381 tccaggccac gatacagcga tgccacctgg tcggcgtaac cattaaacag tagcgttggc
     4441 tggcgctgaa catcgaggat gccgcctgaa ccaataaatc gttcggtagc ctgtgaccag
     4501 cgcgggtgat caacatgcgg attaacgttg gcgtaaaaac cgtattcgtc aggcgctgcc
     4561 agattccagg tggttggcgg acgctcgcgg gtcagcttaa tactgactat cgatttaatc
     4621 cctttaaagc catatttcca cggtacaatc agtcgtaccg gcgcgccatt ttgtggcggt
     4681 aacgccttgc cataaacacc cacggtcatc agtgtgagcg gatgcattgc ttcgtcgaga
     4741 cgcaatcctt cgacataagg atatttcagc ccgccgccga taaagcggtc ctgctggcct
     4801 ggcatttgtt ccggtgcata aattgtttcg aaagcgacat acttcgcatt gctggtgggt
     4861 tcggcaagcg ccagcaattt gtgcagcgga aaaccaatcc acggcaccac catcgaccat
     4921 gcctccacgc agcgcatacg ataaatacgc tcttccagcg ggaagcgacg ggttaaatca
     4981 tcgtgatcga gggtcaatgg ttttgccact tcgccgctga ttttcagtgt ccatggatcg
     5041 gttttcaggc taccagcatt agcggcggga tcggctttat ccagcccgaa ttcatagaag
     5101 ttgttataac cggagacttt atctactggc gtcagtggca ggttattttg ccaggcggca
     5161 ggcttgctga actccagcgg ttttccggcg ggggcgggcg ggcgatcgtt ccctttaaac
     5221 cagctaagca gatcggcatg cgcagcgtga ggcaaagaaa gtgcagctgc gctgatgccc
     5281 agtgctttta acacctgtcg acgcttcata aagaataccg actcggccgt aacatctgat
     5341 tcttttaaaa attgattctt tttcataagc catcacactt ttttgtcgtt agccagcaag
     5401 agccgtttac tgttaatctg tcagggagtg ccctccctaa cagtagcaga gatctgcttt
     5461 tacatattta tgcattaact gccacgatag gttgaatacc catattggct aagtaataaa
     5521 ggcacatgat aatgctcgtt cactttatta atatgaaatt caacggggat ctcagggaag
     5581 aaactttcaa gattttgttt cttgaaatag tccccggttt taaatacgac acggtaatcg
     5641 ccagtagtcg cagtttgctc gggccacagt gccttaattc gtccatcctt atctgttttg
     5701 gcggtattaa gttgtaacca gccgttgtcc gctttctttt caagagtgac tgtcacgtcg
     5761 gcagcgggtt ttccggtttg ctggttcaaa atgtgcacgc taagaatgtt ttgttgtgcg
     5821 gcataaacca aagaaggtaa tgaaaatgct gccgttacta cggagagtac taaataacgc
     5881 tttaacatgt ttatatcctt gtcatgtgaa tgagttttga ggatagcgaa aaagcggcca
     5941 gggtaacctt acagcagcat tacaattttg taatgaacca ggctgtttct ataacatatg
     6001 atttatggca tattattttc atgaagattt tacttattga agataatcaa aggacccagg
     6061 agtgggtaac gcaggggctt tccgaagcgg gttatgtcat tgatgccgtt tctgatggca
     6121 gagatgggct ttatcttgcg ctgaaggatg attatgcatt gatcattctg gatattatgc
     6181 ttccgggtat ggatggctgg cagatcttac aaacgttaag aacagcaaag caaacccctg
     6241 ttatttgcct tactgcaagg gattctgtcg atgacagagt cagagggctg gacagtggag
     6301 caaatgatta tctggtaaaa cctttttcat tttctgagtt gctggcaagg gttcgggcac
     6361 aattaaggca acatcacgct ttgaattcaa cattagaaat cagtggctta agaatggact
     6421 ctgtaagtca aagtgtgagc agggacaata tcagtattac actgacgcgc aaggagtttc
     6481 agttactttg gctactggcc tccagagctg gcgaaattat acccagaacg gttattgcga
     6541 gtgaaatttg gggaatcaac tttgatagtg ataccaatac ggtggatgtc gccattcgca
     6601 ggctccgcgc aaaagttgat gatccttttc ctgaaaagct aatcgccaca atccggggga
     6661 tgggctattc attcgtagcg gtaaaaaaat aatgaaaaga ttatctataa ccgtccgttt
     6721 aaccttgctt tttatattgc tgctgtctgt tgctggcgct ggaattgtct ggactctcta
     6781 taatggcctg gcaagtgagt tgaaatggcg cgatgataca acactcatta accggacagc
     6841 acagatcaag cagttgttaa ttgatggggt aaatccagat acgttacctg tatactttaa
     6901 ccggatgatg gatgttagtc aggatatctt gatcattcat ggtgatagca tcaataaaat
     6961 tgttaaccgg acaaatgtca gtgatgacat gttaaataac atacctgcta gtgagacaat
     7021 cagcgcagct ggcatttaca gaagcattat taatgatact gaggttgatg ctttacgaat
     7081 taatattgat gaagtttcgc catcattaac ggttactgtg gctaaattgg cttcagccag
     7141 acataacatg cttgaacagt ataaaattaa tagcattata atttgcattg tcgccattgt
     7201 actttgctca gtattaagtc cgctgttaat cagaacggga ttacgagaga tcaaaaagtt
     7261 gagtggtgta acggaagcgc tgaattataa cgatagccgg gagcctgttg aggttagcgc
     7321 attaccgaga gaactaaaac ctcttgggca ggcgttgaat aaaatgcatc aagccttagt
     7381 caaagatttt gaacgcctaa gtcaatttgc tgacgatctc gctcatgaac ttagaacgcc
     7441 cattaatgca ttactgggtc agaatcaggt tacgctcagt caaaccagaa gtatcgctga
     7501 atatcaaaaa acaattgccg gtaacattga agagctggaa aatatttcgc ggttaacaga
     7561 gaacatactg tttcttgccc gggcagataa aaacaatgtt ttggtgaaac tggactcgct
     7621 ttctctcaat aaggaagtcg aaaatttgtt ggattatctt gaataccttt cagacgagaa
     7681 agagatttgc tttaaggtcg agtgcaatca gcaaatcttt gcggataaaa ttttactgca
     7741 acgaatgtta tcgaatctta ttgttaatgc cattagatat tcaccagaaa aatcgcatat
     7801 tcatataacc agttttcttg ataccaacgg ctatcttaat attgatgtcg ccagtcctgg
     7861 aacgaaaatt catgagcctg aaaaactctt ccgtagattt tggcggggag ataattcgcg
     7921 tcattccgta ggtcagggac taggcctttc tttagtcaaa gcgattgccg aattacatgg
     7981 gggaagtgct acgtatcact atctcaataa gcataatgtg ttccggatta cgttaccgca
     8041 aagaaattaa cataaggctt cagtacgcca taaattaacc gtgaaaaaac gcagccacgt
     8101 cgtgagtact aacgcggctg cgattgatta gcgcttacat tcaaacgtaa cagggattaa
     8161 cccgcgtaag ctgccagcat ttcctgcgcc gccagtttac ccaacgcatt cgctgcaaaa
     8221 ggactgtcgc cagtgagaac tttacggtcc ttatgtactc gcccggtgat gtcgtcatta
     8281 ataatgttca tgcccatttt cttcagctct tcgccgaagt accaggtgag atgacccggc
     8341 atatagccta tctctggcgt ttgtttgtct gcggcgtctg ggaatgcgca aatggaataa
     8401 ccattcagtg ggttatcgcc gtggcgaagc gccagaaaag ccgccgggcc gtggcaaagg
     8461 gagataacaa aacggtcatt tttgattgcc cactgcaaag cagcagctac gtcctggctt
     8521 tctggtaaac caataagtgc gccatgacca ccaggaacaa agattgctgc atattcgcta
     8581 tcagcgttga ggctggcgac aacatccgcg agtttcttcg gattgcggaa caacgattta
     8641 tgttgctcaa agaatggcat tactttttca tctttgtgcg gcatagccca gtattcaaac
     8701 ttggtcatca gaccggaaat ggtcgccact tcgaattcga aacctgcagc atggagatga
     8761 tacaacggca gcaacgtttc aatcggatgg ttaccggtcg agaacagttt tccgttatcg
     8821 gttggcaaat aacgttcgtc cgcggcgatc accagaattt tatgcttacc gcgatacggt
     8881 tttggatagt ccacgccatc aagatcagag acgggactgg tatattggct aagcgaatat
     8941 tctgaaggga agaatgcatt atcttcagca atatcgacct gcggattttt acttgtttga
     9001 acagtcatag tgtattcctt attgttgctt agttagggta gtcactatat ttgcgactga
     9061 gctggtcgct atttttaacc agtctggaaa attgagtcaa tatatcgaaa gcggtagtcg
     9121 gcgggtgtaa gtatgtaatg atgatgctgc actggtgttg aatattattc acgccaaatt
     9181 ttccttattg agcaacgtcg tcgcaagtgg cctcaatgat tttagaccat tatcaaaggt
     9241 acggggcggc ggggagagat ttagtgctgt gaaatattgt ggtactgatg aatatttata
     9301 tcgcggattg gtagagaaaa gattatttca tgcactattt ttgcaaatat ttctatatgg
     9361 gaagcgtgtt tgttacgggg taattcatca gaatgtgcat ttggggtgtt ggctggaaga
     9421 gtcgctggta gatgttattg tggcaggagg ggcagggtaa ggtgctgtat cgatatgctt
     9481 tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaaagcc tgagttcgat
     9541 gagggacgaa ctcaggctag tgttatgttt tacagtaaca cgccgcggcg cgttggctga
     9601 ttagaactgg tagacgatac caactgcaaa ctggtcatca gttgcggcat cggtaatcgc
     9661 tgcatcgtct ttatccagca ggttaaattt gtatgctgtg tagacgttca tgttcttatt
     9721 gaagtagtac caggtaccaa cttcgatata atttacgcgg tcagcatcaa cacctttatg
     9781 tccattacga gcataaagat ctttgccttt ggaatatacg tagcctaaag acggacgtaa
     9841 accgaagtcg aactgatact gtacaacagc ttcaaagttt tgggttttat ttgcaaaatg
     9901 atcattagcc aaaacggtca tgttgcgggt ttcaccgtac atcattgctg catagatatt
     9961 atttgcatca tacttggctg ctaatgacca cacttcagcg ttatcaccgt agccgtcacc
    10021 agcttgttca ttagtacgat cagatttgct atatgcacct acaaaaccaa aaccttcgat
    10081 gttgtagtct acagacaacg catagccatc accgttttgt ttagtaacgc cacgattgct
    10141 gttcttacct tgatattgca cagcaaaatt cagaccatca accagaccaa agaaatcaga
    10201 gttacggtag gttgcaacac cgttggtacg accgttcatg aagttgtcag cggaagccca
    10261 ggaatcacca ccccattcaa ctaacatatc ggtataagct gcggcatcat aagcaacacc
    10321 gtagttacga ccatagtcga aagatcctaa ctcgttatat ttcaaacctg cgaaggttaa
    10381 acgggttttt tcctgattag agccttcggg cttgctggcg tcgaggttgt attcaaactg
    10441 accaaaacca gtcagttcgc tggtgatttg ggtttcacct ttaacaccga aacgagcata
    10501 ggaggtatct tcgttttcgc tgttattatc atcagtattt gaccagatgc gttcgccaac
    10561 catcttgccg tagaaatcaa ctttattgcc atctttattg taaatttcag ccgcatttgc
    10621 tgcgccagca actaataacg ccgggaccag cattgccaga acttttcttt tcattatgta
    10681 ttcccttgtg attataatct tcatgaatat atcaataagt gccgttatcc aaaaaaaagc
    10741 acatttggat actattctat gaagttcatt ttattttaaa gattacatgt aacaaatgta
    10801 tttaaaagat ttcaaaatgt ttctaatcta tttaatataa ttcaggatta tgaattctaa
    10861 atata
//
LOCUS       DAZFXT010000151        10650 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.151, whole genome shotgun sequence.
ACCESSION   DAZFXT010000151 DAZFXT010000000
VERSION     DAZFXT010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10650
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.151"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            201..1568
                     /gene="dcuC"
                     /locus_tag="ACHKW0_004188"
     CDS             201..1568
                     /gene="dcuC"
                     /locus_tag="ACHKW0_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7476927.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(1611..2108)
                     /gene="sspB"
                     /locus_tag="ACHKW0_004189"
     CDS             complement(1611..2108)
                     /gene="sspB"
                     /locus_tag="ACHKW0_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HFO7476928.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(2114..2752)
                     /gene="sspA"
                     /locus_tag="ACHKW0_004190"
     CDS             complement(2114..2752)
                     /gene="sspA"
                     /locus_tag="ACHKW0_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein SspA"
                     /protein_id="HFO7476929.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(3147..3539)
                     /gene="rpsI"
                     /locus_tag="ACHKW0_004191"
     CDS             complement(3147..3539)
                     /gene="rpsI"
                     /locus_tag="ACHKW0_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HFO7476930.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(3555..3983)
                     /gene="rplM"
                     /locus_tag="ACHKW0_004192"
     CDS             complement(3555..3983)
                     /gene="rplM"
                     /locus_tag="ACHKW0_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HFO7476931.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(4202..5329)
                     /gene="zapE"
                     /locus_tag="ACHKW0_004193"
     CDS             complement(4202..5329)
                     /gene="zapE"
                     /locus_tag="ACHKW0_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HFO7476932.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            5523..5921
                     /gene="zapG"
                     /locus_tag="ACHKW0_004194"
     CDS             5523..5921
                     /gene="zapG"
                     /locus_tag="ACHKW0_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Z-ring associated protein ZapG"
                     /protein_id="HFO7476933.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            6075..7442
                     /gene="degQ"
                     /locus_tag="ACHKW0_004195"
     CDS             6075..7442
                     /gene="degQ"
                     /locus_tag="ACHKW0_004195"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HFO7476934.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            7532..8599
                     /gene="degS"
                     /locus_tag="ACHKW0_004196"
     CDS             7532..8599
                     /gene="degS"
                     /locus_tag="ACHKW0_004196"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HFO7476935.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(8661..9599)
                     /gene="mdh"
                     /locus_tag="ACHKW0_004197"
     CDS             complement(8661..9599)
                     /gene="mdh"
                     /locus_tag="ACHKW0_004197"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030060 - L-malate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HFO7476936.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            10034..10504
                     /gene="argR"
                     /locus_tag="ACHKW0_004198"
     CDS             10034..10504
                     /gene="argR"
                     /locus_tag="ACHKW0_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HFO7476937.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         2631 a         2701 c         2643 g         2675 t
ORIGIN      
        1 ctattcacga gcattatgaa tattatgaat gtgttcttac aaaataatca taagcgcata
       61 ttttttaatg aaaaatcacc tcacctacaa ttaaaaacac gacatccgca ccataaatag
      121 acttgcaaaa aatataacat cattgttttc aatctgccgt ttatgggatt gaccgttttc
      181 ttttgacacg gagttcaaca atgttcggca taattatatc tgtcatcgta ttaattacga
      241 tgggctattt gatcctgaaa aactacaaac ctcaggtggt gctggctgcc gcaggtatct
      301 tcctgatgat gtgcggtgtc tggttagggt tcggtggtgt actcgatccc gccaaaagca
      361 gcggctactt gatcgtcgat atttataatg aaatcctgcg catgctgtcc aaccgcattg
      421 ccggattggg gctgtcgatt atggcggtgg gcggttatgc ccgctacatg gagcgcacag
      481 gagccagtcg cgcgatggtg agcttgctaa gccgcccgtt aaaactcatt cgctcgccgt
      541 atattattct gtcggcaact tacgtcatcg gccaaatcat ggcgcagttt attaccagcg
      601 cctccggcct gggtatgttg ctgatggtca ccttatttcc gacgctggtg agtctgggag
      661 taagtcgcct ctctgcggtg gcggttatcg caaccacgat gtccattgag tgggggattc
      721 tggaaacgaa ctccattttt gcagcccagg tcgcgggaat gaaaattgcc acttacttct
      781 tccactacca gcttccggtc gcctcttgcg tcattatctc ggtggcgatc tcccactttt
      841 tcgtgcaacg cgcttttgac aaaaaagata aaaatatcaa tcacgaacag gcagagctaa
      901 aagctctcga taatgtcccg ccgctctatt acgccatttt acctgtgatg ccgttaatct
      961 tgatgctcgg ctcgctgttc ctcgcccaca tcgggctgat gcagtcagaa ctgcatctgg
     1021 tggtggtgat gttactgagt ttgacggtga cgatgtttgt tgagttcttc cgcaagcata
     1081 acttgcgcga aacaatggac gatgtgcagg cgttttttga cggcatgggt acgcagtttg
     1141 ccaacgtggt aacgctggtg gtcgcgggtg aaatatttgc gaaaggctta acgacgattg
     1201 gcactgttga tgcggttatc aggggtgctg agcattctgg tctgggcggt attggcgtga
     1261 tgattattat ggcgctagtc attgccattt gtgccattgt gatgggctct ggcaatgcgc
     1321 cgtttatgtc atttgccagt cttattccga atatcgcagc cggactacat gtaccagcgg
     1381 ttgtaatgat tatgccgatg cattttgcca cgacgctagc gcgcgcggtt tcgccgatta
     1441 ctgcggtggt ggtcgttacg tcaggaattg caggcgtttc gccttttgcg gtggtgaagc
     1501 gaacagcgat ccccatggca gtcggtttcg tggtgaatat gattgccaca atcacgctat
     1561 tttattaagc cattaaaaga caaaacaggc caccagggcc tgtttttgta ttacttcaca
     1621 acgcgtaatg ccggtcgacc accgcgtggt ggctgcggag gttcatcgtc aggatgagtg
     1681 tcatcatcgt gatctggctt gtcgccatca ataaccgaca taacggtttc gttgtctgcc
     1741 gatgcctctt catcattcat gatgctggta tcttcatcgt aggcagcttc aggctcaaac
     1801 atcgtgcctg cgccattttc acgggcgtag atagccagca cggcagccag cggcacagaa
     1861 acctgacgcg gaatgccgcc aaagcgcgcg ttaaagcgta cctcatcatt cgccagttcc
     1921 agattgccga cagcacgcgg cgcaatgttg agtacgattt gcccgtcacg cgcatattcc
     1981 ataggaacct gcacgccagg gagcgtcaca tccaccacca agtgcggcgt gagctggtta
     2041 tccagcaacc actcatagaa tgcacgcagc agatagggac gacgtggtgt tagctgtgac
     2101 aaatccatac agattaactc cggcccagac gcatttcacg ttctgcttca gttaaagaag
     2161 caaggaaaga gtcacgctca aagacgcggg tcatatagcc tttcagctct ttcgcacccg
     2221 ggccgctgaa ctcgatgccc agttgcggca gacgccacag cagcggagca agatagcaat
     2281 cgaccaggct gaactcatcg ctcaggaagt acggcttctg accgaagacc ggcgcaatcg
     2341 ccagcagttc ttcgcgcagt tgcttacgtg cggcatctgc ttcagaagct gaaccgttga
     2401 tgatggtgtt catcagcgtg taccagtctt tttcgatgcg atgcatgtac agacggcttt
     2461 caccgcgagc taccgggtaa acaggcatca gtggcggatg cgggaaacgc tcatccagat
     2521 attccataat gatgcgagat tcccacaggg tcagctcacg atccaccagg gtcggaacgc
     2581 tctgattcgg gttgaggtca atcagatcct gaggcggatt gtccttttcc acgtgttcga
     2641 tctcgaaact tacacctttc tcagccagca caatgcggac ctgatggcta tagatgtcag
     2701 taggaccgga aaacagcgtc attaccgaac gtttgttggc agcgacagcc atgaaaacct
     2761 ccaggtatag tcagaatttt tactgctacc agccaccagg tggccagtca gaagttgtgt
     2821 tacccagtaa ggaacgactc tctttgttcg aaaatcaaac aaaaaatgag caatacccga
     2881 catttgggca gaaaattgga tgatagttta ccagattttg cgacctttgt ggtgagtcga
     2941 tgccggaaat ggggaaaaag agatgcgctt tagtctgaaa tagttgactt agtcccttat
     3001 tggcgatgtg gtttttgttt tgcctgtctg tcagacggta gcaaaaagca actttccaat
     3061 ttttacgctg attcagaatt taaccataaa aaaacccgcc gaagcgggtt ttttcgcaaa
     3121 ttgttttctg ccggagcaga agccaattaa cgtttggaga actgcggacg acgacgtgct
     3181 ttacgcagac cgactttctt acgttcaacc tgacgagcgt cacgagtaac gaagccagct
     3241 ttacgcagtt cagaacgcag ggactcgtcg tattccatca gagcgcgggt gataccgtga
     3301 cggatcgcac cagcctgacc agagatacca ccacctttaa cggtgatgta caggtccagt
     3361 ttctcaacca tgtcgaccag ttccagcggc tgacgaacta ccatgcgagc agtttcacga
     3421 ccgaagtact gttccagaga acgttggttg attacgattt taccgttgcc cggtttgatg
     3481 aaaacgcgag ctgcggaact tttgcggcga ccagtgccgt agtattgatt ttcagccatt
     3541 gcctataatc ccgattagat gtcaagaact tgcggttgct gtgccgcgtg gttgtgctcg
     3601 ttacccgcgt aaactttcag tttacggaac atagcacgac ccagcgggcc ttttggcaac
     3661 atgcctttaa ccgcgatttc aatcacacgc tcaggacggc gagcaatcat ctcttcaaag
     3721 gtagcttgtt tgataccacc gatgtggccg gtgtggtgat agtacacttt gtcagtacgc
     3781 ttgttgccgg ttacagcaac tttgtcagcg ttcagaacga tgatgtaatc accggtatct
     3841 acgtgcggag tgtattccgc tttgtgctta ccgcgcaggc gacgagccag ttcagtagcc
     3901 agacggccca gagttttacc ggtcgcgtca acaacatacc agtcgcgttt tacggtttct
     3961 ggtttagctg taaaagtttt cattaaaagc ttacccaata aatagttaca cgttggtgaa
     4021 cacccaaacg tcttcaattg ttgaggttca cacgacaaag tccggcaaac ctaccccttc
     4081 gaatagccta tgccagcaca caaaaagttt tgggaaaaaa actttcttgt aacgtggggt
     4141 cgcaggatta tagagaagtc ggggtcaaag atcgacccct ttttgtgatt tgtgacaggt
     4201 tttaacccgc caaatgctcg cgcttcagat actcttcgct ttgcatctct tgcagacgtg
     4261 acaggcaacg ctggaactca aacttcagcc gatcgccctg ataaatttca tacagcggca
     4321 cttctgcact caccactaat ttgacatggc gctcgtaaaa ctcatccacc agcgcaataa
     4381 agcgccgcgc ttcgctctcc atcaaccgcg tcataactgg tacatcaaac aacatgaccg
     4441 tatgaaagag acgtgagagc gcaatatagt catgctgact gcgggcgtcg acgcacagcg
     4501 tagtaaaaga gaccgccagc gtctggttct cgacgcccat tgtcgccaat ggccgatggt
     4561 tgatttctaa cgtcggtgaa ttttctcgtt tcgcccccgc cagcgccaac catagtttat
     4621 ccatctgcgc ccgggtttca tcgttgagtg gcgaaagcca cagatgcgcc tgagtgagtg
     4681 tacgcagtcg ataatcaaca ccagcgtcca cgttcattac atcacaatgc tgtttaatgg
     4741 catcgattgc aggcagaaaa cgcgcacgtt gcaggccatt tcgataaagt tcatccggcg
     4801 gaatatttga cgtcgctacc agggtaatgc cgcgggcgaa cagggctttc atcagaccgc
     4861 caagtagcat ggcgtcggta atatcagaaa caaaaaattc gtcaaaacag agcacgtcag
     4921 tttcggcttt aaagcgatcg gcaataattt ccagcggatc ggtctgcccc tgtaaggcag
     4981 ttagctcttc gtgcacccgc agcataaaac ggtgaaagtg caggcgctgt ttccgctctc
     5041 ccggcaggct ttgatagaaa aggtccatca gccaggtttt cccgcgtcct acaccgcccc
     5101 acatatataa gccacgcact ggcgtatgct ttgtgtcttc gcgtttaccc cacagcttac
     5161 cgacccgcgc cattagccca ctcgtcctgg gggctggtgg cctgctattg atgagttctt
     5221 gataaataat ttccaggcgg ctgacggcct ctttttgaac gtcgtcgggt tgatggctgc
     5281 cttcattgag cgccttcagg tattgcgatg ttggggtaac gctttgcatg atcttattgt
     5341 tattccttga ataatcggtg cgccgttgtt cacggttgac gaaaaaaagg ccgttctaca
     5401 ctacgcgata tgcagtcggg attccacttc tgtggaatta acggttatag tggcataatc
     5461 agccgcaggc atggagcctg aagccaacac cctacggaaa caaaagacaa cgggagatgt
     5521 tcatgacctg ggaatatgcg ctaattgggt tagtcgtcgg catcattatt ggtgctgtgg
     5581 ccatgcgttt tggtaatcgt aaactacgcc agcaacaggc gttgcagtac gaactggaaa
     5641 agaataaagc tgaactggac gagtatcgcg aagagctggt tagccacttt gcccgcagcg
     5701 cggaattact ggataccatg gcgcacgact atcgccagct gtatcagcac atggcgaaaa
     5761 gctccagcag cctgctgccg gaactgtctg ctgaagcaaa cccgttccgt aatcgtctgg
     5821 cagagtctga agccagcaac gatcaggcac cggtgcagat gcctcgcgac tattctgaag
     5881 gtgcatccgg cctgctgcgt actggcgcga agcgcgacta atttattttt cgggcgcagc
     5941 cattgcgccc tcctcttctc tccctccccg actatcattt aatctggtgt ctcattgtta
     6001 gccgtctgaa aattcaataa catcaaactg ttttgaatct cttttcttat cattcaggta
     6061 cgagagcagg aataatgaaa aaacaaaccc agctgttgag tgcattagcg ttaagtgtcg
     6121 ggttaactct ctcggcgtca tttcaggccg tcgcgtcgat tccaggccag gttgccgatc
     6181 aggcccctct ccccagtctg gctccaatgc tggaaaaagt gcttccggca gtggtgagcg
     6241 tacgggtgga aggaacggcc agtcagggac agaaaatccc ggaagaattc aaaaagtttt
     6301 ttggtgatga tttaccggat caacctgcac aacccttcga aggtttaggt tccggtgtca
     6361 tcatcaacgc cagtaaaggc tatgtgctga ccaacaacca tgtgattaat caggcacaga
     6421 aaatcagtat tcagctcaat gatgggcgcg agtttgatgc aaaactgatt ggtagcgatg
     6481 accagagcga tatcgccctg ttacaaattc aaaacccgag caaattaacg caaatcgcta
     6541 ttgccgactc cgataaattg cgcgtcggtg attttgccgt agcggtcggt aacccatttg
     6601 gccttgggca aaccgccacc tctggcattg tttccgcatt aggccgcagc gggttgaatc
     6661 ttgaaggtct ggaaaacttt atccagacag atgcttccat taaccgcggt aactccggcg
     6721 gtgcactatt aaaccttaac ggtgagttaa ttggcatcaa cactgcaatc cttgcgcctg
     6781 gcggcgggag cgtcgggatt ggatttgcca tccccagtaa tatggcgcga acactggcgc
     6841 agcagcttat cgactttggt gaaatcaaac gcggtttgtt aggcatcaaa ggcaccgaga
     6901 tgagtgccga tatcgccaaa gccttcaacc ttgacgtgca gcgtggcgcg tttgtcagcg
     6961 aagtgttgcc aggttctggc tcggcaaaag cgggcgtcaa agcgggcgat attattacca
     7021 gcctcaacgg caaaccgctg aatagctttg ctgagttgcg ctctcgtatc gcgaccaccg
     7081 agccgggcac gaaagtgaag cttggcctgc tgcgtaacgg caaaccactg gaagtagaag
     7141 tgacgctcga taccagcacc tcttcgtcgg ccagcgctga aatgatcacg ccagcgctgg
     7201 aaggtgcaac gttgagcgat ggtcagctaa aagatggcgg caaaggtatt aaaatcgatg
     7261 aagttgtcaa aggaagccca gctgctcagg ctggcttgca aaaagacgat gtgatcattg
     7321 gcgtcaaccg cgatcgggtg aactcgattg ctgaaatgcg taaagtgctg gcggcaaaac
     7381 cggccatcat cgccctgcaa attgtacgcg gcaatgaaag catctatctg ctgatgcgtt
     7441 aatgtcgtaa accgggcatc aggcttacgt gtgatgtccg gttaactcgt ggtatgctgc
     7501 tgccgttccc ttttttaatg acgcctccat catgtttgtg aagctcttac gttccgttgc
     7561 gattggatta attgtcggcg ctattctgct ggttgccatg ccttcgctgc gcagccttaa
     7621 cccgctttcc actccgcaat ttgacagtac cgatgagacg cctgccagct ataatctggc
     7681 ggttcgccgc gccgcgccag cggtggttaa cgtttacaac cgtggtttga acaccaactc
     7741 tcacaaccag cttgagatcc gcaccttggg atccggtgta atcatggatc aacgcggtta
     7801 tatcatcacc aataaacacg tcatcaacga cgccgatcag atcatcgtcg ccttacagga
     7861 tggtcgtgta tttgaagcat tgctggtggg atctgactct ctaaccgatc tggcggtact
     7921 taaaattaat gccactggcg gtttacctac cattccaatt aatgcacgtc gcgtaccgca
     7981 cattggcgac gtagtactgg cgatcggtaa cccgtacaac ctcgggcaga ccattaccca
     8041 ggggattatt agtgccacgg gtcgaatcgg tctgaacccg accgggcggc aaaacttcct
     8101 acaaaccgat gcttccatta accacggtaa ctctggcggc gcgctggtga actcgctggg
     8161 agaactgatg ggcattaaca cgctgtcgtt tgataagagt aacgatggcg aaacgccgga
     8221 aggtatcggc tttgcgattc ctttccagtt agcaaccaaa attatggata agctgatccg
     8281 cgatggtcgc gtgatccgcg gctacattgg tatcggcgga cgcgagatcg caccactgca
     8341 cgcgcagagc ggtggtatag atcaactgca agggatcgtg gttaatgaag tgtcaccaga
     8401 cggcccagcg gcgaatgcgg gtattcaggt caacgatctg attatttcgg tggataacaa
     8461 accggccatc tctgctctgg agacgatgga tcaggtggcg gaaattcgcc ctggttcggt
     8521 gatcccggtt gtagtgatgc gtgatgataa gcagttaacg ctgcaggtca ccattcagga
     8581 atatccggca accaattaag tcgagtgctc aaaacaaaaa ccggagtctg tgctccggtt
     8641 ttttattatt cgctaatcaa ttacttatta acgaactctt cgcccagggc gatatctttc
     8701 ttgagcgtat ccagcatacc ttccagcgcg ttctgttcaa atgcgctcag ggtaccgata
     8761 gatttacgct cttccacgcc gtttttaccc agcagcagcg gttgagagaa gaaacgggcg
     8821 tactgaccgt cgccttcaac gtaggcacat tcgacaacgc cttgttcgcc ctgcagtgca
     8881 cgaaccagag acagaccaaa acgtgcagct gcctggccca tagacagggt tgcagacccg
     8941 ccaccggctt tcgcttcaac cacctcagta cccgcgttct ggatacgttt ggtcagatca
     9001 gccacttcct gctcggtaaa actaacgcca ggaacctgtg acagcagcgg cagaatggta
     9061 acaccagagt gaccgccaat aaccggcact tcaacttcgc ctggctgttt gcctttcagt
     9121 tccgcaacaa aggtgttgga acgaatgata tccagcgtgg taacgccgaa cagtttgttt
     9181 ttgtcataaa caccggcttt tttcagcact tcagcagcaa tcgcaactgt ggtgttaacc
     9241 gggttagtga taataccaat gcacgctttc gggcaggttt tcgcaacttg ctgtaccagg
     9301 tttttcacga tgccggcgtt aacgttaaac aggtcggaac gatccatacc cggtttacgc
     9361 gctacacctg cagagataag aacgacatct gcgccttcca gcgccggagt cgcatcttca
     9421 ccagaaaaac ctttgatttt cacagcagta gggatatggc tcagatcgac agccacaccg
     9481 ggagtcactg gagcgatatc atacagagag agttctgaac ctgaaggcag ttgggttttt
     9541 aacagtagtg caagcgcctg gccaataccg ccagcagcgc cgaggactgc gactttcatc
     9601 ctaaactcct tattatattg ataaactaag atatgttgct ccgctgccgc gaccttaatc
     9661 cacaaaattg ccgtttacaa tgaccacatc tcaagaatgt gtagtcacgc aagtttagcg
     9721 tttatgcatt taattgccgt aatcaggaac ctaacttacg taattaacag tcgcttatgc
     9781 attagcgcaa cattccaaca ggtggtgaca atatacccta ccgttcagcc aaaacaacat
     9841 caatttgata acaattaatt tacttttaag cagaatttgc atgccgtgac gcaggcatgt
     9901 ttctcaataa cgaaatttga taaaatcccg ctctttcata acattatttc agccttcttc
     9961 agggctaact gtttgcataa aaattcatct gtatgcacaa taatgttgta tcaaccacca
    10021 tatcgggtga cttatgcgaa gctcggctaa gcaagaagaa ctagttaaag catttaaagc
    10081 attacttaaa gaagagaaat ttagctccca gggcgaaatc gtcgccgcgt tgcaggagca
    10141 aggctttgac aatattaatc agtctaaagt ctcgcggatg ttgaccaagt ttggtgctgt
    10201 acgtacacgc aatgccaaaa tggaaatggt ttactgcctg ccagctgaac tgggtgtacc
    10261 aactacctcc agtccattga agaatctggt gctggatatc gactacaacg atgcagttgt
    10321 cgtgattcat accagccctg gcgcggcgca gttaattgct cgcctgctgg actcactggg
    10381 caaagcagaa ggtattctgg gcaccatcgc tggcgatgac accatcttta ccacccctgc
    10441 taacggtttc acagtcaaag acctgtacga agcgatttta gagctgttcg accaggagct
    10501 ttaatctctg ccccgtcgtt tctgacggcg gggaaaatgt tgcttatccc tctcaatccc
    10561 ctgctttccc ctgcgattca tttaacgaat agtgcgtttt accgcgacat atcattcacg
    10621 caatgaatac atgcggtaaa aaaagcacat
//
LOCUS       DAZFXT010000152        10606 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.152, whole genome shotgun sequence.
ACCESSION   DAZFXT010000152 DAZFXT010000000
VERSION     DAZFXT010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.152"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            70..786
                     /gene="yeeN"
                     /locus_tag="ACHKW0_004199"
     CDS             70..786
                     /gene="yeeN"
                     /locus_tag="ACHKW0_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HFO7476938.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
     gene            complement(1238..1313)
                     /locus_tag="ACHKW0_004200"
     tRNA            complement(1238..1313)
                     /locus_tag="ACHKW0_004200"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1278..1280),aa:Asn,seq:gtt)
     gene            complement(1414..2901)
                     /gene="yeeO"
                     /locus_tag="ACHKW0_004201"
     CDS             complement(1414..2901)
                     /gene="yeeO"
                     /locus_tag="ACHKW0_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HFO7476939.1"
                     /translation="MWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITP
                     LAVPIFMENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVV
                     VAFSLGKRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALA
                     LTYLELTVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSW
                     PGLGFVGAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIG
                     IPASVESVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITG
                     RRLGVGQIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGLMASFYTQDPQVKHVVVILI
                     WLNALFMPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVG
                     VWMGMFADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPAVSE"
     gene            3062..3137
                     /locus_tag="ACHKW0_004202"
     tRNA            3062..3137
                     /locus_tag="ACHKW0_004202"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3095..3097,aa:Asn,seq:gtt)
     gene            complement(3175..4125)
                     /gene="cbl"
                     /locus_tag="ACHKW0_004203"
     CDS             complement(3175..4125)
                     /gene="cbl"
                     /locus_tag="ACHKW0_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HFO7476940.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(4227..5144)
                     /gene="nac"
                     /locus_tag="ACHKW0_004204"
     CDS             complement(4227..5144)
                     /gene="nac"
                     /locus_tag="ACHKW0_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HFO7476941.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            5471..5546
                     /locus_tag="ACHKW0_004205"
     tRNA            5471..5546
                     /locus_tag="ACHKW0_004205"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5504..5506,aa:Asn,seq:gtt)
     gene            complement(5601..6536)
                     /gene="ldtA"
                     /locus_tag="ACHKW0_004206"
     CDS             complement(5601..6536)
                     /gene="ldtA"
                     /locus_tag="ACHKW0_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HFO7476942.1"
                     /translation="MMRRVNIFCSFALLFASHTSLAVTYPLPPEGSRLVGQSFTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFISGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(6598..7677)
                     /gene="cobT"
                     /locus_tag="ACHKW0_004207"
     CDS             complement(6598..7677)
                     /gene="cobT"
                     /locus_tag="ACHKW0_004207"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HFO7476943.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(7689..8432)
                     /gene="cobS"
                     /locus_tag="ACHKW0_004208"
     CDS             complement(7689..8432)
                     /gene="cobS"
                     /locus_tag="ACHKW0_004208"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0051073 - adenosylcobinamide-GDP
                     ribazoletransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HFO7476944.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGAPLAALFSVLVLVLMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(8429..8974)
                     /gene="cobU"
                     /locus_tag="ACHKW0_004209"
     CDS             complement(8429..8974)
                     /gene="cobU"
                     /locus_tag="ACHKW0_004209"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HFO7476945.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPEHWRTVERWQHLDELIHADINPNEVVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQVINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(9126..9299)
                     /locus_tag="ACHKW0_004210"
                     /pseudo
     CDS             complement(9126..9299)
                     /locus_tag="ACHKW0_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
     gene            complement(9278..9466)
                     /locus_tag="ACHKW0_004211"
                     /pseudo
     CDS             complement(9278..9466)
                     /locus_tag="ACHKW0_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT         3031 a         2693 c         2225 g         2657 t
ORIGIN      
        1 aatatgccga attaacacat gttaacgatt taccagtaat gtaaataaat tttcgaggag
       61 atcattccag tgggacgtaa atgggccaat attgttgcta aaaaaacggc taaagacggt
      121 gcaacgtcta aaatttatgc aaaattcggt gtagaaatct atgctgctgc taaacaaggc
      181 gaacccgatc cagaattaaa cacatcttta aaattcgtta ttgaacgtgc aaagcaggca
      241 caagttccaa agcacgttat tgataaagca attgataaag ccaaaggcgg cggagatgaa
      301 acgttcgtgc agggacgtta tgaaggcttt ggtcctaatg gctcaatgat tatcgctgag
      361 acattgactt caaatgttaa ccgtacgatt gctaacgttc gcacaatttt caataaaaaa
      421 ggcggcaata tcggagcggc aggttctgtc agctatatgt ttgacaatac gggtgtgatt
      481 gtatttaaag ggacagaccc tgaccatatt tttgaaattt tacttgaagc tgaagttgat
      541 gttcgtgatg tgactgaaga agaaggtaac attgttattt atactgaacc tactgacctt
      601 cataaaggaa tcgcggctct aaaagcagct ggaatcactg agttctcaac aacagaatta
      661 gaaatgattg ctcaatctga agttgagctt tccccagaag atttagaaat ctttgaaggg
      721 cttgttgatg cccttgaaga tgacgacgat gtacaaaaag tttatcataa cgtcgcaaat
      781 ctctaattat attttaaaga aatctgtctt tacggcagat ttctttaatc tcatataacc
      841 cttataaaaa atataatgtt caactcgtca tattgattat aaaaaatccc cgttcccaga
      901 gaaataatat ttattaaaat tccagttctt ctttttctga ttacagaagg caaagtggca
      961 ataacgcata gtttcccgat aaagacgcga tagcgacatc ccgcataagg catttttctc
     1021 tttatctttg tacggtactt catggaacag agtttttgac cttgcgaatc gtgatgtctg
     1081 ttgggggcaa cttgctcact gaagcgtgag actcgattaa gcgcacgaaa cacagaaatc
     1141 aaaaaacccg gtcacttttt tacaaggtaa ccgggtaaaa ataattttta ttttttaact
     1201 gttttgagac tcatagagat gtctcaaaac taaaatttgg ctcctctgac tggactcgaa
     1261 ccagtgacat acggattaac agtccgccgt tctaccgact gaactacaga ggaatcgtga
     1321 gaacgaggcg aatattagcg atgcccaccc ataatgtcaa agcctgtttt ttaaatttga
     1381 aatcgtttgc tgaaataatc tgcattttgt cgtttattcc gacacagctg gctttttttc
     1441 acacttttgc ggctcggatc gagggtattt ccatagccaa cgtccagtaa ccattcgcca
     1501 gtaaaacagc acggcccgca cagcccagtc ggcaaacatt cccatccaga caccaaccac
     1561 accccagcca agcatgatgc ccagcacata accgacgaca acccggcaac cccacatgct
     1621 caacatcgaa acccacatgg cgtaacgggc atcacgagca cctttaaatc cagcgggtag
     1681 cacccatgag gcggaccaaa taggcataaa taaagcattt agccaaatca gaatcacaac
     1741 gacatgttta acctgtggat cctgggtgta aaacgatgcc ataagcccgg caaagggagc
     1801 cgttagccag gcgatggccg ttaatccaag agtggacagc cagaacacat gccgcaactg
     1861 aatctctgct tgcgctatct gccctacccc caaccttcgg cctgtaatga tcgtagaagc
     1921 agagccgagc gcacttccgg gtaagttgat aagagccgca attgaaaacg cgataaaatt
     1981 tccggcaata acactggtcc ccatcccggc aacgaacatt tgggttaata accgaccact
     2041 ggtaaataac actgattcga cactcgctgg aataccaatc cccatgactt cccagataat
     2101 gctaaaattc agcggtttaa aatagctctt taacgaaatc cttagcgcag gattaaaacc
     2161 aatcgccagc acccacaaaa ttgcaactgc gccaatataa cgagaaatgg ttaaacccaa
     2221 cccagccccg acaaatccca gtcccggcca ggagaaaagg ccgtaaatca atatgccgct
     2281 aataataata ttaagaatat tcaggctacc gttaatcagt agcggtattt tcgtattccc
     2341 tgcaccacga agtgccccgc taccaatcaa agtgatggca gctgctggat aactgagtac
     2401 cgtcagctcc agataagtca acgccagtgc tttaacttct gtcgtggcat cacccgcgac
     2461 gaaatcaata atttgttcgc caaaatgatg aataagcgtt gccaacagta cggcaaacaa
     2521 cgtcatgatc accaatgact gccgcgtcgc caccctcgct cgtcgtcgat cccgcttacc
     2581 gagactaaat gccacaacga cagtagtacc aagatcgata gctgcaaaaa aagccataat
     2641 gaccatattg aagctgtccg ccaatcccac gccggccatc gcatcttttc ccagccagct
     2701 gaccagaaaa gtgctcagga cacccatcaa caggacacag gcattctcca tgaagatagg
     2761 aacagcaagc ggggttatct cgcgccagaa caacactttg tagctcttgc gtttagcgtg
     2821 ccagcgagtg ccgtgaacaa cctggcgtaa agcagaggag atattcaaag ccgaccttaa
     2881 ttgcagaaag tgaaaccaca tttcaaataa tgagggagaa tcagcaaagc tgcaaagatt
     2941 ttcgccaaca aattgtctgc aaatgcaaca aactgttgat agaaacggca aacaattggg
     3001 gaatttaaaa atcgggtttg acaaaagatt tttcgccgtt aagatgtgcc tcaacagcga
     3061 ttcctctgta gttcagtcgg tagaacggcg gactgttaat ccgtatgtca ctggttcgag
     3121 tccagtcaga ggagccaaat ttctgaaaag cccgctttta tagcgggctt tttgctatat
     3181 ctgataatca atttcctctt cactgttttc catcacctgc cgcttgatat cctcaactga
     3241 cagtcctgca ttacaaagtt ccagaaagcg ccagacatag ttacgctgaa gttgtcctcg
     3301 cttcagtccc aaccagacag tattagcatc gaaaagatgc cgcgtatcca gacggattaa
     3361 attctcttcc tcttgttcgc cgctggattg ttcggcaact aatccgatcc caagcccaag
     3421 agcaacatag gttttaatga catcagaatc ctgcgcactt aatacaatat ctgccagcaa
     3481 acctttgcgg gcaaatgcgt catcaatacg tgagcgcccc gtaatcccct gtcggtaagt
     3541 gattaacggc cacttcgcta ttgattccag cgtcaatggt gaaatttgcg tcaagggatg
     3601 atcgtgtgga acaagcaaac tatggtgcca acgaaaccac gggaaggcga cgagctgcgg
     3661 gtcattactc aaacgctcgc tggcgatacc aatatcagct tcgccatttt gcaacaatgt
     3721 cgcaatttcc tgtggcgtcc cctggattag ctcgagccga acctccggga aaagttcgcg
     3781 aaaagcttta atgacctctg gcaagctata acgtgcctga gtatgcgtcg ttgcaatagt
     3841 gagaacgcca gacgtatcgt tggtaaacag gtctgcaagc cgacgaacat tactggcttc
     3901 attcagaata cgttctgcaa tgaccagtaa tgctttgccc ggttcagtca tgcccagcag
     3961 tcgcttacct cgtcgaacaa atatttcgat gccaagttca tcctccagtt cccgaatatg
     4021 acggctgacg cctgactgtg aggtaaaaag catattcgca acctctgtca ggttgtaatc
     4081 ctgacgtgca gcctcgcgga ttatctttag ttgttggaaa ttcacggtaa actccgggca
     4141 gttcagattt cccgttattg ttaaagtcta atgcccggca taacaaataa taaaaacccg
     4201 catcttattc catcccgata taacacttag ctcaccaatt gccactgcct tttttccatc
     4261 actggagaac taatcactga cattaacaac tctttcactg cctgtgcctg tggcgataag
     4321 ttcgctctgg cgggtaaatt taatgacaaa gagagactca tggaaggagt ggtaatgcgt
     4381 gacatccacc catttactgc gccacataac gaacgcgcgg ccgattcggg taatactgca
     4441 acgcccatgc cgctggcaat cgctgcggta agcgtggcaa tagactcaat ttcaccaata
     4501 acttttgccg tgagtcgccg tagggaaaaa gcctcatcaa cacgaagtct aatagcactg
     4561 taatcactgg ggagaaagag gttcattttc gcaatagcat tcacatcaac gctttgcccc
     4621 gggcaatctt gagttcctac cagaaaaaga tcttctttca gcaaagcctg actggataca
     4681 ccagccacag gggaatgctc ataaatcacc gccatatcga gttggtgatt tatcaatttt
     4741 tcgttaagca ctgcaccact attttcatga agatagataa cgatctccgg aaattcagcg
     4801 cgaaccgcct gtaataaggg catggtgatg gatgacgcag cggttcctgg tgcaaagcca
     4861 atcgagactt gccccgataa tgcctgacca acgttatgca ccgccagttg ggcctgttca
     4921 cactgacgta aaatggcccg cgcatgggta tagagaattt ttccggcgtc tgttggcgta
     4981 acgccccgct ttgtacggat caaaagttgt tgatttaact caccttccag tgtggcaacc
     5041 tgctggctga gcgctggttg tgcgatatgc aatacttcag cagcctgggt caggctacca
     5101 atatctacaa tttttacgaa gtatttcagg cgtctgaagt tcatgttgcc tccggttttt
     5161 aagaatcggc ccaagtgccg ctattactta caaccagatt gcaagatgct tgccagtttt
     5221 attttggcgt tgatgtacaa gctaaccaac tgtcaaataa gagattatga tagattcgtc
     5281 atttgctcct ttaatcagct gtcgcgttcc cctgccctat aaaaggaggg tatgcaccac
     5341 gatggttcat tacccaataa gattgaaagc tcaccacttt gttgaaattg acagcaaaca
     5401 aacaaaaaaa tgcatttcac cctttgacat caccatgcac tgccattaat atgcgccccg
     5461 ttcacacgat tcctctgtag ttcagtcggt agaacggcgg actgttaatc cgtatgtcac
     5521 tggttcgagt ccagtcagag gagccaaatt caaaaaagcc tgctttctag caggcttttg
     5581 ctttctaatt accaacgctc ttaaaacatc tgtcttgaac cagaactaat ttgcacaggc
     5641 attcccgatc gacgttgcaa cgcagcattt gcgcgattta catcaacttc ttgcccgctg
     5701 ataaacgccc gcaaagatgg ggttaccggc aatggcactt ttcggtcaga ctcatattct
     5761 gcacgattgc gcgacaacgg ctcatgaact tccagccagt tcgagccatc tggttcagtg
     5821 gtgtatttta ctggctggtc gataatttgc acacgcgtcc caacaggaac attatcaaac
     5881 agatatttga tatcgtcatt gcgcagacga atacagccct gacttacccg gagcccaata
     5941 ccaaaattgg cattggtacc atggatggca tacaacctgc caatataaat cgcgtacagc
     6001 cccatgggat tatcggggcc cgcaggaaca aatgcgggca aactctcccc tcgtttcgca
     6061 tattcgcgcc gagtgttcgg cgttggcgtc caggttggag cttcttgttt acgttcaacg
     6121 gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc cgataccaat aggaaagact
     6181 tccacagtat tactgtctgg tgggtagtaa taaagacgca tctcagcgac gttaaccaca
     6241 atccctttac gaacagtggc gggcaaaatc agttgctgcg gaatggtgag ttgcgagcca
     6301 gacttcggca aaaaaacatc agcgcccggg ttcgcttcca gcatgttact taacccttgc
     6361 ccgtattgtg cggcaaaagt ctccagcggc tgggtattgt gatcaggaac agttacagta
     6421 aacgactgcc ccactaaacg gctaccctct ggaggtaatg gataagttac agccaggcta
     6481 gtatggctgg caaaaagcag agcaaatgag caaaaaatat ttacacgacg catcatgtcc
     6541 ctttcctaag tcgcgaaagc tatccgttaa gtatagcttt tatcagactt ttcgttttta
     6601 actgttcaaa tcagaagtcg tattccccgg tagaacaata ttactggcag caagttcgcc
     6661 catgttgtta tatatcgcac aggcagcttc gatgatgggc atcgccagag ctgcgccact
     6721 cccctcacct aaacgcatct ccatattgag ataaggctcc agccccaaat gcgacagcgc
     6781 aatacgcgcc cctttttctg ccgacaggtg agaaggaatg agatacggtt tgattgcagg
     6841 agacatctgg caggctgcga gcgcggcagc ataagaaaga aatccatcca gcaacacggg
     6901 taaaccgcag gaagcagcgc ctaacatcac gccagccatt ccgaccaaat caaatccacc
     6961 cacttttgcc aggacatcaa cgccatcctg cggatttggt tgattcaacg taatcgcccg
     7021 gcgcacaaca tcaattttat tagccagttt atctgtcggc aggtttgcgc caatcccaac
     7081 cacttcttca ggagcccggc cagtgattgt gctgactatt gccgctgccg gtgtcgtgtt
     7141 tgccatcccc agttcaccta caccaaacag cgtaacaccg ttttttgcca gctcccgcgt
     7201 ataacatatg acgtccaaaa gcaacttttc tgcctgatgg cgactcattg ccggagctga
     7261 agcaatattg ccactacctc gagcaacacg catgttgata agcccgggga taggctcagc
     7321 agtatcaata ccaacatcaa ttacgtagac gttagcgccc gcttgtgctg ccagcacaca
     7381 cacgccggtt gttccgcggg tcatattttc agcctgaatg gctgtcactt cttttgggga
     7441 aatagcgacc ccttcctccc agacgccgtg atcggcacac ataaccagta ccgctttttt
     7501 gcccacatgc ggtatgccat tcaaccccgg cattcctgcc agttgtatgg caagcgcctc
     7561 cagccttccc aggctaccaa caggtttgag taacccgtca atatgccgtt gtgcacgcga
     7621 catcgcggca ggatcgatgg cagggatcgt attcagtaaa tcggcaagtg tttgcatgtc
     7681 acgtctcttt atagcagtgc cagcaggaag accagttcac caagctctat agcagctccc
     7741 agcgtatcgc cggtttgccc gcccagcgta cgtttgagaa gctgaccgag gatgaaaatc
     7801 gccaccatcg tcaccaccat agcagccaca ccatgcatac ccggcaataa tacggcagca
     7861 aaaatagcgg ctaagccgag tgtgacgcag gtttgtcgcc catcaatttt gccaatgaac
     7921 acattgccaa gcccttcttc ccgggcataa cgatgacgat acatcaataa tgcggcagtc
     7981 ccgcggctga ccgcacatgc tgccgccagc gatgcaagga tcggttcgcc acgcagagcc
     8041 aactcgctta acaccagaat ctttgccagt accacaaaaa tcaatgccag accgccgtgg
     8101 gtgcctaaac ggctatcacg catgatttcc aacatgcgat cgcggctacg tgcagaaaat
     8161 acgccatcac aggtatcagc gaggccgtcc agatggaatc ccccggtcat cagcaccagc
     8221 acaagtacgc taaacagtgc cgccagtggt gcgccacacc atgcctgcag caccatgaag
     8281 accagcccgc taatcgcgcc aagcaataat ccaatcaaag gaaaagtaat aataccgcga
     8341 gaataatgct cgaaatccag tccctgggac cagcgacgcg gtacaggcag acgcgtaata
     8401 aatgagagca tcgcccaaaa taatttactc atttgatttt tactccaata cccgaaacca
     8461 ccagccatac ttcatttgcc gcagcggcca actgctgatt tacccgcccg gcaatatcac
     8521 gaaaatgtcg tgccagacga ctctccggca caatccccat tcccacttcg ttagtcacta
     8581 atacaacctt tgcggggcaa cgttggcagg cagcaatcaa cgactgaatc tcagcattaa
     8641 tcacctgttc catcgcctga taatcccatt catcagggtc tttatcgccg ccataatcaa
     8701 acaacagatt agtcaccatt gtggtaacgc attcaagcaa cacaacctca ttcgggttaa
     8761 tgtctgcatg aattaattca tcaagatgtt gccagcgctc cactgtgcgc cagtgctccg
     8821 ggcggccttg ccgatgatgt tctatccgtg cagccatctc atcatcaagg atttgcgagg
     8881 tagcgatata cagaacctgt gaagagtccc caataagcgc ctctgcgtgg cgactcttcc
     8941 cgctccgtgc gccccccgtc accaaaatca tcataccgac tcctgattca aatgtatgat
     9001 ggtattcatt gaacagaaca atggatcccc caccgttgct gaccgcaaaa catttacgcg
     9061 attaatactg cgcgtaatat aattaaaaat ccagcattct caatccatta cgaaataaga
     9121 tggcattacg gaatagccag actttctcgt gttaacgtcc gacaaaatgc gtcattgaca
     9181 gcggtttctg tggattgaca catttcattc agataatgaa ttaatgctac tgccggaaca
     9241 accagcaaac gggcattgtg ttctgaaatc caggctattg attcaacgtc agcgacagat
     9301 ctgcgctgta aaagaacttg taaaacaacg taatagaatt atccggaatg gtggcgacta
     9361 tgcactagaa caccacaatt tcgctctctc ggtaaggcac tgtcacagca ttcaacagaa
     9421 tgtgacttgc cagatttatt agcgccacca gatgtttaac cgggtaacca cgacccagtc
     9481 ggggatatat tccatcttct cgctgacatc atcaagaata aaaaggttta tcacactcag
     9541 agcaacgttc ttcagcaggc gggataattc ttcaacattt agaagatgtg ttattaaagg
     9601 catacaaact ggacgtattg tattttcttt tggtggttga cctaaggtag cagtttatcc
     9661 tgatgcgctg agatttctgt aatatctacg tcaacattct cctcgaatag aaaatgcagc
     9721 cccggcaagt gattcacatt ttttaacaaa acgttgttag cgtgccattt tcagaagctc
     9781 tccaagaaag cgaattcttt tctcaaattc tgcattctca gacacagctt tctactccat
     9841 ggtaatggcc caaatacgaa gttgctcagg atcattagtg gtgctgagaa gttaaataac
     9901 catgcggagt accactcgtc atcacgtatt tatatatcat gaagctattg ttgtgttttg
     9961 tacatcagta gatatattct gatatactcc ttttgctaga cataaccttt cacctgcttg
    10021 caaagcttct gtgttctgac attgccaaat tgttgcaatt ctgtatccag ccttctttca
    10081 gtcatagctt cgggccgcga taagactcac tgatctgacc ctgattcctc ttgcagactt
    10141 tatagaccaa ttaaaatgca gtttctgcag gtcaacgtct gaccatcatt gtcatcactc
    10201 tggccattag agtaaccttc tgcattcatc cttttgtaaa aagtttatat tagtatcagc
    10261 aattaaccgg acctgatact gatatgagtc ttaccgcata tacggtcaat ttcagcaatt
    10321 aattacatta tccacgccaa agtatttgtc atcacaatga tggtaccttc tttcagacac
    10381 cattttttca actccgtttt ccacggaccg cactcttatg tcaagagtgc ggtccgtgga
    10441 tacaaccaga gaccgactga cacgagtcag aggaaacgac ggatatgttc agtcgtaaaa
    10501 tatctatcaa aaaacatgat taaggtcaaa aatgtttgat atttacaatt tatgaagatg
    10561 acaataatta tagatatatg agaacataaa tgaaaataat tatcat
//
LOCUS       DAZFXT010000153        10523 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.153, whole genome shotgun sequence.
ACCESSION   DAZFXT010000153 DAZFXT010000000
VERSION     DAZFXT010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10523
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.153"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..154)
                     /locus_tag="ACHKW0_004212"
     CDS             complement(<1..154)
                     /locus_tag="ACHKW0_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7476946.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPG"
     gene            complement(241..309)
                     /gene="ykiE"
                     /locus_tag="ACHKW0_004213"
     CDS             complement(241..309)
                     /gene="ykiE"
                     /locus_tag="ACHKW0_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkiE"
                     /protein_id="HFO7476947.1"
                     /translation="MGWSLCFWHVSVKMTHDVVCYY"
     gene            446..967
                     /gene="fecI"
                     /locus_tag="ACHKW0_004214"
     CDS             446..967
                     /gene="fecI"
                     /locus_tag="ACHKW0_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HFO7476948.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            964..1917
                     /gene="fecR"
                     /locus_tag="ACHKW0_004215"
     CDS             964..1917
                     /gene="fecR"
                     /locus_tag="ACHKW0_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric citrate uptake sigma factor regulator
                     FecR"
                     /protein_id="HFO7476949.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGASGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADVRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            2004..4328
                     /gene="fecA"
                     /locus_tag="ACHKW0_004216"
     CDS             2004..4328
                     /gene="fecA"
                     /locus_tag="ACHKW0_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent Fe(3+) dicitrate receptor FecA"
                     /protein_id="HFO7476950.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGITLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPALAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMDAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRSYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYGSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWTF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVAYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGSLKF"
     gene            4373..5275
                     /gene="fecB"
                     /locus_tag="ACHKW0_004217"
     CDS             4373..5275
                     /gene="fecB"
                     /locus_tag="ACHKW0_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HFO7476951.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIAPVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            5272..6270
                     /gene="fecC"
                     /locus_tag="ACHKW0_004218"
     CDS             5272..6270
                     /gene="fecC"
                     /locus_tag="ACHKW0_004218"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HFO7476952.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            6267..7223
                     /gene="fecD"
                     /locus_tag="ACHKW0_004219"
     CDS             6267..7223
                     /gene="fecD"
                     /locus_tag="ACHKW0_004219"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HFO7476953.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPHTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIYPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            7224..7991
                     /gene="fecE"
                     /locus_tag="ACHKW0_004220"
     CDS             7224..7991
                     /gene="fecE"
                     /locus_tag="ACHKW0_004220"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HFO7476954.1"
                     /translation="MTLRTENLTVSYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGHLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(8527..8931)
                     /locus_tag="ACHKW0_004221"
     CDS             complement(8527..8931)
                     /locus_tag="ACHKW0_004221"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HFO7476955.1"
                     /translation="MMRGILPPVSVCRMAIPWWITRIHGVTTSAPLITGAGGGVGVSI
                     GAGGNGAGISVFASVNAAKGSEKGNGTEWTETTTDSGKTVTINSGRDTVLNGAQVNGN
                     RIIADVGHDLLISSQQDTSKYDSKQTSVAAGD"
     gene            complement(8813..9841)
                     /locus_tag="ACHKW0_004222"
                     /pseudo
     CDS             complement(8813..9841)
                     /locus_tag="ACHKW0_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0046930 - pore complex [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
     gene            complement(9940..>10523)
                     /locus_tag="ACHKW0_004223"
     CDS             complement(9940..>10523)
                     /locus_tag="ACHKW0_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO7476956.1"
                     /translation="VPPSKRAHTGRVAVKESNQRWCSDGFEFRCDNGEKLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNELPASPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKSTAVRSPESNGIAESFVKTIKRDYISVMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALGYRSPREYLRQQASNGLSDNRCLEI"
BASE COUNT         2288 a         3039 c         2872 g         2324 t
ORIGIN      
        1 taccgggctg agaggcggtg taagtgaact gtagttgcca tgttttacgg caatgagagc
       61 agagatagcg ctgatgtccg gcagtgcttt tgccgttacg caccacgcct tcagtagcgg
      121 agcaggaagg acatctgatg gaaatggaag ccacgcaagc accttaaaat caccatcata
      181 cactaaatca gtaagttggc agcattaccg aaatccccat cctttagggc agtgagaatg
      241 tcaataatag cagaccacat catgagtcat tttgaccgac acatgccaga agcagaggct
      301 ccatcccatt ttatacctac cctataacac ttagaaaaac aacatgttaa aaatgtctat
      361 tggaaacaat tttatttcca attgtaatga taaccattct catattaata tggctacgtg
      421 ataattaact tttgatgcac tccgcatgtc tgaccgcgcc actaccacag cttccttaac
      481 gttcgagtcg ctttatggca cacatcacgg ctggttgaaa agctggctga cgcgcaaact
      541 ccagtctgct tttgatgcag atgacattgc ccaggacact tttttgcggg taatggtcag
      601 cgaaacgctc tcgacgatcc gcgatcctcg ctccttcctg tgcactatcg ccaaacgcgt
      661 gatggtggac ctgtttcgcc gaaacgcgct ggaaaaagcg tatctggaga tgctggcgct
      721 tatgccggag gggggagcgc cttcacctga ggaacgcgaa agccaactcg agaccctaca
      781 actcctcgac agcatgctgg acgggctaaa cggcaaaaca cgtgaagcgt ttctgctttc
      841 gcaactggat ggtctgacat acagcgagat tgcgcacaaa ctcggtgttt ccatcagctc
      901 cgtgaaaaaa tacgtggcga aagccgtcga gcactgcctg ctgttccgtc tggagtatgg
      961 gttatgaatc ctttgttaac cgattcccgc cgtcaggcgc tgcgttcagc ttcccactgg
     1021 tatgccgtgc taagcggcga gcgcgtcagc ccacaacagg aagcgcgctg gcaacagtgg
     1081 tatgaacagg atcaggataa ccagtgggcc tggcagcagg ttgaaaacct gcgcaaccag
     1141 cttggcggtg tgcctggcga cgttgccagc cgggcgttgc acgatacccg cctcacccgc
     1201 cgtcacgtga tgaaaggatt actgctgttg ctcggcgcta gcggaggctg gcagctctgg
     1261 cagtcggaaa ccggcgaagg tctgcgggca gattaccgca ccgccaaagg cacagtcagc
     1321 cgtcagcaac tggaagatgg ctccctgctc acgctgaata cccaaagcgc ggcggatgtg
     1381 cgttttgatg cgcatcagcg caccgtccgg ctctggtacg gtgaaatcgc cattactacc
     1441 gcgaaagatg ccctgcaacg ccccttccgt gtcctgaccc gtcagggcca gctcactgct
     1501 ttagggacag aatttaccgt ccgccagcag gataatttca cgcagcttga cgtgcagcag
     1561 cacgccgtgg aagtgcttct cgccagtgcc cccgcgcaaa aacgcatcgt gaacgctggt
     1621 gaaagcctgc agttcagcgc ctctgagttt ggcgcagtga aaccgctgga tgacgagagt
     1681 acaagctgga cgaaggacat cctgagcttc agcgataaac cgctgggtga ggtgatagcc
     1741 acgctaaccc gttaccgcaa cggcgtgctg cgctgcgatc ccgccgttgc cgggctgcgc
     1801 ctgagcggga cgttcccgct gaaaaatacc gatgcgatcc tgaacgttat cgcgcaaacg
     1861 cttcccgtta aaattcagtc tattacgcgg tactggataa acatttcacc actgtaagga
     1921 aaataattct tatttcgatt gtccttttta cccttctcgt tcgactcata gctgaacaca
     1981 acaaaaatga tgatggggaa ggtatgacgc cgttacgcgt ttttcgtaaa acaacacctt
     2041 tggttaacgc cattcgcctg agcctgctgc cgctggccgg tctctcgttt tccgcttttg
     2101 ctgcacaggt taatatcgca ccgggatcgc tcgataaagc gctcaatcag tatgccgcac
     2161 acagcggaat taccctctcg gttgacgcca gcctgacgcg cggcaagcag agcaacggcc
     2221 tgcacggcga ttacgacgtc gagagcggcc tgcaacaact gctggacggc agcggactgc
     2281 aggtaaaacc gctgggaaat aacagctgga cgctggagcc cgcgctcgca ccaaaagaag
     2341 atgccctgac cgtggtcggc gactggctgg gtgatgcgcg tgaaaacgac gtatttgaac
     2401 atgctggcgc gcgtgacgtg atccgccgtg aggatttcgc caaaaccggc gcaaccacca
     2461 tgcgtgaggt acttaaccgc atccctggcg tcagcgcgcc ggaaaacaac ggcaccggca
     2521 gccacgacct ggcgatgaac tttggcatcc ggggcctgaa cccgcgcctc gccagccgct
     2581 cgaccgtcct gatggacggc atccccgtcc ccttcgcccc ttacggtcag ccgcagcttt
     2641 cactggctcc cgtttcgctc ggcaacatgg atgccattga cgtggtacgc ggtggtggtg
     2701 cggtgcgtta cggaccgcag agcgtgggcg gcgtggtgaa ctttgttacc cgtgccattc
     2761 cgcaggactt tggtatcgag gcgggcgtgg aaggtcagct cagcccaacc tcttcacaaa
     2821 acaacccgaa agagacgcac aacctgatgg tgggcggcac agcggacaac ggttttggca
     2881 ccgcgctgct ctactccggc acgcgcggca gtgactggcg cgagcacagc gccacccgca
     2941 tcgacgacct gatgctgaaa agcaaatatg cgccggatga ggtgcacacc ttcaacagcc
     3001 tgctgcaata ttacgacggt gaagccgaca tgcccggtgg cctgtctcgc gcggattacg
     3061 acgccgatcg ctggcaatcc acccgctcgt atgaccgctt ctggggtcgt cgcaagctgg
     3121 cgagcctggg ctaccagttc cagccagaca gccagcataa attcaacatt caggggttct
     3181 acacccaaac cctgcgcagc ggctacctgg agcaaggcaa acgcatcacc ctctcgccgc
     3241 gtaactactg ggtgcgcggt attgagccac gctacagcca gatctttatg atcggccctt
     3301 ccgcgcacga agtgggcgtg ggctatcgct atgtgaatga atcaacgcat gaaatgcgtt
     3361 actacaccgc caccagcagc gggcagttgc cgtccggctc aagcccttac gaccgcgata
     3421 cgcgttccgg caccgaggcg cacgcctggt atctggatga caaaatcgac atcggcaact
     3481 ggaccatcac gccgggtatg cgtttcgaac atatcgagtc ataccagaac aacgccatca
     3541 caggcacgca cgaagaagtg agctataacg caccgcttcc ggcgttgaac gtgctctatc
     3601 acctgactga cagctggaat ctttatgcaa acactgaagg ctcgttcggc accgtacagt
     3661 acagccagat tggcaaggct gtgcaaagcg gcaatgtgga accggaaaaa gcgcgaacct
     3721 gggaactcgg tacccgctac gacgacggcg cgctgacggc ggaaatgggg ctgttcctga
     3781 ttaactttaa caatcagtac ggctccaacc agaccaacga caccgtcact gcacgtggca
     3841 aaacgcgcca taccgggctg gaaacgcagg cacgttacga tctgggtacg ctaacgccaa
     3901 cgcttgataa cgtttccatc tacgccagct atgcgtatgt gaacgcggaa atccgcgaga
     3961 aaggcgacac ctacggcaat ctggtaccat tctccccgaa acataaaggc acgctgggcg
     4021 tggactacaa gccaggaaac tggacgttca atctgaacag cgatttccag tccagccagt
     4081 ttgcggataa cgccaatacg gtgaaagaga gcgccgacgg cagtaccggc cgcattcccg
     4141 gcttcatgct ctggggcgca cgcgtggcgt atgactttgg cccgcagatg gcagatctga
     4201 acctggcatt cggtgtgaaa aacatcttcg accaggacta cttcatccgc tcttatgacg
     4261 acaacaacaa aggcatctat gcaggccagc cgcgcacgct gtatatgcag gggtcgttga
     4321 agttctgatg tgttaacgcc cggcttgccg ggcttttagc tggaatgtga ttatgttggc
     4381 atttatccgt tttctttttg caggcctgct gctggtgatc agccacgcct ttgccgccac
     4441 ggttcaggac gaacacggca cgtttacact cgaaaaaacg ccacaacgga ttgtggtgct
     4501 ggaactctcg ttcgccgatg cgctggccgc cgtggacgtc agcccgatcg gtattgccga
     4561 cgataacgat gcaaaacgca tcctgcccga agtgcgtgcg cacctgaaac cgtggcagtc
     4621 cgtcggaacg cgcgcgcagc cgagcctgga agccattgcc gctctgaaac cagacctgat
     4681 cattgccgac agcagtcgcc atgcgggggt ttacatcgcc ttgcagcaaa tcgcgccggt
     4741 actgctgctt aagtcccgca acgaaaccta cgctgaaaat ttgcaatctg cggctatcat
     4801 cggcgaaatg gtgggtaaaa agcgagagat gcaggcacgt ctggaacaac ataaagagag
     4861 gatggcgcag tgggccagcc agcttcccaa agggacacgc gtggcctttg gcacatcacg
     4921 cgaacagcaa ttcaacctgc atactcagga gacctggacc ggcagcgtgc tggcctctct
     4981 ggggctgaac gttcccgctg cgatggcggg tgcgtccatg ccgtccatcg gcctggagca
     5041 actgctggcg gtcaatcctg cctggctgct ggttgcccac tatcgcgaag agagcattgt
     5101 taaacgctgg caacaagatc cgctctggca gatgttaacc gccgcgcaga agcagcaggt
     5161 tgcttcggtc gacagtaaca cctgggcgcg gatgcgcggt atttttgctg cagagcgtat
     5221 tgccgctgac acggtaaaaa tcttccacca tcagccgctt accgttgtga aatgaccgcg
     5281 ataaaacacc cggtgctgct gtgggggctt cccgttgcag cacttattat tattttctgg
     5341 ctgagtctgt tttgctactc ggccattcct gtttccggag cagatgcaac ccgcgccctg
     5401 ctgcctggac acacgccaac gctaccagaa gcgctggtgc aaaaccttcg tttgccacga
     5461 agcctcgtcg ccgttctgat cggcgcaagc ctggcgctcg cgggcacgct gctgcaaacc
     5521 ctgacccaca acccaatggc ctctccttca ctgctcggca ttaacagcgg cgcggcgctg
     5581 gctatggcgc ttaccagcgc gctgagtccg acgccgattg caggctattc tctgtcgttc
     5641 atcgcggcat gcgggggcgg cgtgagctgg ctgctggtca tgaccgcagg aggcggattt
     5701 cgtcataccc atgacagaaa caaactgatc ctcgcgggta tcgcgctgtc ggccttttgt
     5761 atgggcctta cccgcatcac cctgctgctg gccgaagatc atgcttacgg catcttttac
     5821 tggctggcag gcggagtgtc ccacgcccgc tggcaggatg tctggcagct cttgccggtg
     5881 gtggtcactg cagtccctgt cgtgttgctg ctggcgaatc aactgaacct gctcaacctc
     5941 agcgacagca ccgcccatac gctgggagtg aacctgacga ggctacgttt ggtcatcaat
     6001 atgttagtgc tgcttctggt tggtgcgtgc gtcagcgtgg caggtccggt ggcgtttatc
     6061 ggtctgctgg tgccacatct ggcgcgcttc tgggcaggct tcgatcagcg caacgtactg
     6121 ccagtgagca tgctgctggg ggccacgctg atgctgctgg cagatgtact cgcacgcgcg
     6181 ctggccttcc ccggagatct gcccgcaggc gcagtgctgg cgctgattgg cagcccttgc
     6241 tttgtctggc ttgtgaggag gcgaggatga aaattgcgct ggttattttc atcacccttg
     6301 ccctggcggg ctgtgcgctg ttatcactcc atatgggagt gatccccgtg ccgtggcgcg
     6361 cgctgctgac cgactggcag gccggacgcg agcattatta tgtattgatg gagtaccgac
     6421 tgccgcgctt gctgttggca ctgtttgtcg gtgcagccct cgccgtggcg ggtgtgctga
     6481 tacaggggat tgtgcgcaac cctctggcat caccggatat tctcggcgtt aaccatgccg
     6541 ccagcctggc ctctgtgggg gctctacttc ttatgccgtc actgcccgtg atggtgctgc
     6601 cgctgctggc ctttgcgggc ggcatggcgg ggttgatatt gctgaagatg ctggcaaaga
     6661 cccaccagcc gatgaagctg gcgctcaccg gcgtggcgct ttctgcatgc tgggccagcc
     6721 tgacggatta tctgatgctc tcgcgcccgc aggatgtgaa caacgccctg ctgtggctga
     6781 ccggcagctt atggggccgt gactggagct ttgtgaagat tgccatcccg ctgatgattt
     6841 tatttctgcc gctgagcctg agtttttgcc gcgatctcga cctccttgca ctcggcgatg
     6901 cgcgcgccac cacgctcggt gtgtcggtgc cccatacccg attctgggct ttgttactag
     6961 ctgtcgccat gacatctacc ggcgtggccg cctgcggccc gattagcttt attggtctcg
     7021 tggtgccgca tatgatgcgt agcatcaccg gtggacgtca ccgcagactg ctgcctgttt
     7081 cggccctgac aggtgcgttg ctgttggtgg ttgccgatct gctggcgaga attatttatc
     7141 ccccactgga gctcccggtt ggcgtgctga ccgccattat cggtgcgccg tggtttgtct
     7201 ggttgcttgt gagaatgcga taaatgactt tacgaactga aaatctgacg gtcagttacg
     7261 ggacagacaa ggtacttaac gacgtttcac tctcactgcc aacggggaag atcaccgccc
     7321 tgatcggtcc taacggttgc gggaaatcga cgctgttaaa ctgtttttcg cggcttttaa
     7381 tgccgcagtc tggcaccgta tttctcggcg ataatcccat aaatatgctc tcatcgcgcc
     7441 agttggcccg caggctttcg ctgctgcctc agcaccattt aacgccagag gggatcacag
     7501 tccaggagct ggtttcgtat ggtcgtaatc cctggctgtc actctggggg catctctccg
     7561 ctgaagacaa tgcacgagtt aatgtcgcca tgaaccagac ccggattaat catcttgccg
     7621 ttcgtcggtt aaccgagctt tccggcggtc agcgccagcg cgcatttctg gcgatggtcc
     7681 tggcccagaa tacgcccgtt gtattacttg atgaaccaac cacctatctt gatatcaatc
     7741 accaggtgga cctgatgcgg ttgatgggcg aactccggac tcaggggaaa acggtggtcg
     7801 ctgtgctgca cgaccttaat caggctagcc ggtactgcga tcaactggtg gtaatggcaa
     7861 acgggcatgt tatggcgcaa ggcacaccag aagaggtgat gaccccagga ttgctgagaa
     7921 cagtattcag cgtggaagcg gaaatacacc ccgagccggt atctggcagg ccgatgtgcc
     7981 taatgaggta gattgcacag gccgtaagaa ccaaaccacg actgaatgaa actggactgg
     8041 cgccagcaag ccagttcaga ctggggctga acttttccgg actctgaaag attaccaata
     8101 ctcatcgtcc atccgcttgc tttaggctga caggttcata atcaacgcaa accagagctg
     8161 tacaggcttg ggcgcggctt tcaaaccagt cgtgaacggc aatcaatttt gaactctgct
     8221 taacggacat ttctgtataa cccttacggc aacgaaaaac gcgaagttaa aattttagaa
     8281 acccaaaaac gtgacatgac taagtttaga tttcaggggg gagatcaaaa aatttcgctc
     8341 tgtgccagag cggacattca tggagctggt tcattaccaa tgaggttggg cttttgagga
     8401 taaatcaatg atcagacgcc aacgtaaatc aaaagcaacc ctggaacgga attgctaatc
     8461 cagtttctga ccatcgattt ttctaaaaag tgtgcgtttg ctactactta ggtaggtgca
     8521 gctttcttaa tcaccggcag ccacgctggt ctgtttactg tcgtacttac tggtgtcctg
     8581 ctggctgctt atcagcaggt cgtggcccac atcggcgata atcctgttgc cgttgacctg
     8641 agcaccgttc agtaccgtat cccgaccact gttgatggtg acggttttac cgctgtctgt
     8701 tgtggtttca gtccactcag taccgttacc tttctcgctg ccttttgccg cattaacgct
     8761 ggcaaagacg ctgataccgg caccgttacc accggcaccg atactgacac ccacgccacc
     8821 gccagccccg gttatcaatg gtgctgaggt agtcactcca tgaatacgtg taatccacca
     8881 gggtatagcc atacggcaga ctgataccgg cggcaaaatt cctcgcatca tgtgacaccg
     8941 aaaagtcact gctccgtccc ccgctgacaa accagttgtc agccagcccc agaggattat
     9001 taaaggaaag gacaccattt aactgccccg taccggtatt cttctgcccg ctgttgtcga
     9061 tgcccacgct ccctgtgact ggccattccg gcaatgccgt cagtgtcacc accgaccatc
     9121 cctcacggtc accgggcgat atttcaatct gtaccggttc cgtacgcaga cgattaatct
     9181 gctccatccc ctgctcaata tcccgcaggt tcagaacctt cccctccatt cccgggaaaa
     9241 ccatcttcag ggtgcgggca ggaaggtcag cgccttccgc ccggatttgc tgcagcctgc
     9301 cttccatgac cgttatgtgc agtacgcccc ctgaaaggtc ctgctctgtc agaaaggccc
     9361 ggctggtgat atatccccgg cgtatatagc cgtccgtcac ggcatccgtg accgcagtca
     9421 ggccggtgat attcagacac tgattcaccc acggtgccac cagtctgtcg gtttctgcag
     9481 acgtcagtcg ggtggcccca ctgacaacaa tgcgtgacac cgtaaagcag gggccttcag
     9541 taccggcaga cgtttccgga gacggcgtga tagtcagcgg cgcactgcgc tccagcgcat
     9601 cacgctgatg ctggttttca tccagcaact gctgctgttg ctgctgaatt gcactgcggt
     9661 cacccggaga cagcatggct gccggagacg aaaatgcggg gataaaaaaa ccagaaaaaa
     9721 cggagaggga gagatagcat atccacggag agccacgccg gaacatatca caacagggga
     9781 cagacaacgc acgggataaa agggacgttt tcgtcagaat gttgttctgc cggcgctgca
     9841 tgttctcccc gtattctggc ctgtgtgatg ccagtgtaaa ttcatgtcaa aaagtgtcag
     9901 cgagaaaatt tacacctgaa aaagtctgtg gatttgcccc tatatttcca gacacctgtt
     9961 atcacttaac ccattactgg cttgctgccg tagatattcc cgtggcgagc gataacccag
    10021 tgcactatgc ggatgccatt cgttataatg ctcgaacgcc tctgcaaggt tctttgctgc
    10081 cgttaacccg tctggtttgg gcatgacact gatgtagtca cgctttatcg ttttcacgaa
    10141 gctctctgct atgccgttac tctccggact ccgcaccgcc gtgctcttcg gttcaagccc
    10201 caacatccgg gcaaactgcc gtgtttcatt agcccggtag catgaaccat tatccgtcag
    10261 ccactctact ggagacgccg gaagctcgtt gccgaagcgg cgttccaccg ctcccagcat
    10321 gacgtcctgt actgtttcac tgttgaagcc gcccgtagtg accgcccagt gcagtgcctc
    10381 acggtcacag cagtccagcg cgaacgtgac tcgcagtttt tctccgttat cacagcggaa
    10441 ctcgaacccg tcagagcacc atcgctgatt actttctttc acagccactc tgcctgtatg
    10501 tgcccgtttc gatggcggta cag
//
LOCUS       DAZFXT010000154        10488 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.154, whole genome shotgun sequence.
ACCESSION   DAZFXT010000154 DAZFXT010000000
VERSION     DAZFXT010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.154"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            97..672
                     /gene="ssuE"
                     /locus_tag="ACHKW0_004224"
     CDS             97..672
                     /gene="ssuE"
                     /locus_tag="ACHKW0_004224"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_process: GO:0046306 - alkanesulfonate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HFO7476957.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            665..1624
                     /gene="ssuA"
                     /locus_tag="ACHKW0_004225"
     CDS             665..1624
                     /gene="ssuA"
                     /locus_tag="ACHKW0_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HFO7476958.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADTRAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1621..2766
                     /gene="ssuD"
                     /locus_tag="ACHKW0_004226"
     CDS             1621..2766
                     /gene="ssuD"
                     /locus_tag="ACHKW0_004226"
                     /EC_number="1.14.14.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008726 - alkanesulfonate monooxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HFO7476959.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2777..3568
                     /gene="ssuC"
                     /locus_tag="ACHKW0_004227"
     CDS             2777..3568
                     /gene="ssuC"
                     /locus_tag="ACHKW0_004227"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HFO7476960.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3565..4332
                     /gene="ssuB"
                     /locus_tag="ACHKW0_004228"
     CDS             3565..4332
                     /gene="ssuB"
                     /locus_tag="ACHKW0_004228"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HFO7476961.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4539..7151)
                     /gene="pepN"
                     /locus_tag="ACHKW0_004229"
     CDS             complement(4539..7151)
                     /gene="pepN"
                     /locus_tag="ACHKW0_004229"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HFO7476962.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7417..8619
                     /gene="pncB"
                     /locus_tag="ACHKW0_004230"
     CDS             7417..8619
                     /gene="pncB"
                     /locus_tag="ACHKW0_004230"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HFO7476963.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIVHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8788..10188
                     /gene="asnS"
                     /locus_tag="ACHKW0_004231"
     CDS             8788..10188
                     /gene="asnS"
                     /locus_tag="ACHKW0_004231"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HFO7476964.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2377 a         2704 c         2810 g         2597 t
ORIGIN      
        1 aaaatttata tttggcattt tcttgtctct ccgacagact gaccgaattg ttatcaatgt
       61 taacaaaaaa agaacaattg gttataagga gagagtatgc gtgtcatcac cctggcgggt
      121 agtcctcgct ttccttctcg ctccagctcc ttgctggaat atgcgcggga aaaactaaat
      181 ggcctcgatg tagaggttta tcactggaat ctgcaaaact tcgccccgga agatctgctt
      241 tatgctcgtt tcgatagtcc ggcactcaag accttcaccg aacagctgca acaggctgat
      301 ggactgatag tcgctacacc tgtgtataaa gccgcctatt ccggcgcgct gaaaaccctg
      361 ctcgacctgc tgccagagcg cgctttgcaa ggcaaagtgg tgctaccgct ggcgacgggc
      421 ggtaccgtgg cccatctgct ggcggtcgat tatgccctta aaccagtttt aagcgcactg
      481 aaagctcagg agatcctgca cggcgtgttt gccgatgact cacaagtaat tgattaccat
      541 cacagacccc agttcacgcc aaatctgcaa acccgtcttg ataccgcgct agaaactttc
      601 tggcaggcat tgcaccgccg cgatgttcag gttcctgacc ttctgtctct gcgaggtaat
      661 gcccatgcgt aacatcatta aactggcgct ggtgggattg cttagcgtct ctacgattgc
      721 ggttgctgca gaatcctcgc ctgaagcgtt acgtataggc tatcagaaag gcagtattgg
      781 tatggtactg gcaaaaagcc accagttact ggaaaaacgc tatccgcaat caaaaatctc
      841 ctgggtggag ttccccgcgg gtccgcaaat gttggaagcg ttaaacgttg gcagtattga
      901 tctcggcagt accggggata ttccgccaat ctttgcccag gctgccgggg ctgatttggt
      961 atatgtgggc gtcgagccac cgaagcccaa agctgaagtg attctggtgg cagaaaacag
     1021 cccgatcaaa accgtagccg atcttaaagg tcacaaagtt gcctttcaga aaggttccag
     1081 ttcacacaac cttttactgc gtgcactgcg tcaggccgga cttaagttta ccgatatcca
     1141 acccacttac ctgacgcccg ctgatacccg cgccgcgttc cagcaaggta acgttgacgc
     1201 ctgggctatc tgggatccct actactctgc tgcattatta cagggcggcg tgcgggtgct
     1261 gaaagacggc accgatctca atcaaactgg atcgttttat ctggcagctc gtccctatgc
     1321 agaaaaaaac ggcgctttta ttcagggcgt actcgcaacc tttagtgagg ccgatgcgtt
     1381 aacccgcagc cagcgcgagc aaagcatcgc tttactggca aaaacgatgg gcttaccggc
     1441 accggtgatt gcctcttact tagatcatcg ccctcctacc accatcaaac cggttaacgc
     1501 cgaggttgcc gccttacagc agcaaacggc agatctgttt tatgaaaatc gtctggtgcc
     1561 gaaaaaagtc gatattcgcc agcgcatctg gcagcccact caactggaag gaaaacaatt
     1621 atgagtctga atatgttctg gtttttaccg acccatggtg acgggcatta tcttggaacg
     1681 gaagaaggtt cacgcccggt tgatcacggc tatctgcaac aaattgcgca agcggcggat
     1741 cgtctgggct ataccggtgt gctgatccca acggggcgat cgtgtgaaga tgcgtggctg
     1801 gtggcggcgt cgatgatccc ggtgacgcag cggctgaagt ttcttgtcgc cctgcgcccc
     1861 agcgtaacct cacctaccgt tgccgctcgc caggccgcca cgcttgaccg tctctcaaat
     1921 ggtcgtgcgt tgtttaatct ggtcacaggc agcgatccac aagagctggc aggcgacggc
     1981 gtgttccttg atcatagcga gcgctacgaa gcctcggcgg aatttaccca ggtctggcgg
     2041 cgcttattgc tgggggaaac cgtcaatttc aacggtaaac atattcatgt gcgcggagca
     2101 aaactgctct tcccgccgat tcaacagcct tatcctccgc tttactttgg cggatcgtca
     2161 gacgtcgccc aggagctggc ggcagaacaa gttgatctct acctcacctg gggcgaaccg
     2221 ccagaactag taaaagagaa gatcgaacaa gtgcgggcga aagctgccgc gcatggacgc
     2281 aaaattcgtt tcggtattcg tctgcatgtg attgttcgtg aaactaacga cgaagcgtgg
     2341 caggccgccg agcggttaat ctcgcatctt gatgatgaaa ctatcgccaa agcacaggcc
     2401 gcattcgccc ggacggattc cgtaggacaa cagcgaatgg cggcgttaca taacggcaag
     2461 cgcgacaatc tggagatcag ccccaattta tgggcgggcg ttggcttagt gcgcggcggt
     2521 gccggaacgg cgctggtggg cgatggtcct acggtcgctg cgcgaatcaa cgaatacgcc
     2581 gcgcttggca tcgacagctt tgtgctttcg ggctatccgc atctggaaga agcgtatcgg
     2641 gtcggcgagt tgctgttccc acatctggat gtcgccattc cggaaattcc ccagccgcag
     2701 ccgctgaatc cgcaaggcga agcggtggcg aatgatttta tcccccgtaa agtcgcgcaa
     2761 agctaaggaa gaaataatgg caacgccagt gaagaagtgg ttattgcgcg ttgccccctg
     2821 gtttttaccg gtgggcatcg tggcggtgtg gcaactggcc tcctcagttg gctggctgtc
     2881 gacgcgtatt ttgccctcac cggaaggggt ggtgacggcg ttctggacgc tctccgccag
     2941 cggcgaactg tggcagcatc tggcgatcag ttcctggcgg gcgctgattg gcttttcaat
     3001 tggcggatcg ctggggttga ttttgggact gattagcggg ctatctcgct ggggagaacg
     3061 gctgctggat acctcaattc agatgttgcg caacgtaccg catctggcgc tgatcccgct
     3121 ggtgattttg tggtttggca ttgatgaatc cgcaaaaatc tttctggtgg cgctcggcac
     3181 gctattcccc atttatatca acacctggca tggcatccgt aatatcgatc gcggtctggt
     3241 ggaaatggcg cgtagctatg gattatccgg cataccgctg tttatccatg tgatcctgcc
     3301 tggtgccctg ccctcaatta tggtcggcgt gcgttttgcg ttgggcctga tgtggctgac
     3361 gctgattgtt gccgaaacca tttctgccaa ttcaggcatt ggttatctgg cgatgaatgc
     3421 gcgggagttt ttgcaaacgg acgtggtggt agtcgctatt attctttacg ccctgctcgg
     3481 caaacttgct gacgtcagcg cgcagctgtt agagcgcctc tggttgcgct ggaacccggc
     3541 ttatcatttg aaggaggcca cggtatgaat actgctcgtc tgaaccaggg cacaccattg
     3601 ttgctcaatg cagtaagcaa acattacgcg gaaaatatcg tcctgaacca actggattta
     3661 catattccgg caggtcagtt tgtggcggtg gtgggccgca gcggtggtgg caaaagtacc
     3721 ctgctgcgcc tgctggcagg tctggaaacg ccaaccgcag gcgatgtgtt agcgggcacc
     3781 acaccgctgg ctgagattca ggaagacacg cgaatgatgt ttcaggatgc gcgtctgctg
     3841 ccgtggaaat cggtgattga taacgttggg ttaggcctta aaggccagtg gcgcgatgcc
     3901 gcgcgtcggg cgttggctgc agtaggactg gagaatcgcg ccggggaatg gcctgccgca
     3961 ctttctggcg ggcagaaaca gcgtgtggcg ctggcaagag cgttgattca tcgaccgggt
     4021 ttattgttgc ttgatgaacc gctcggggcg ctggacgcct taacgcgact cgagatgcag
     4081 gatttgattg tgtcactttg gcagcagcac ggctttaccg tactgttggt gacgcatgat
     4141 gtgagcgaag cggtagcaat ggctgaccgg gtgttgttaa ttgaagaggg aaaaattggt
     4201 ctggatttga cagtggatat cccccgtccg cgtcgattag ggtcggtgcg attagcggag
     4261 ctggaagcgg aagtattgca acgggtaatg cggcgtgggc attctgaaca actgattcgg
     4321 cgtcatggtt aatatgccgg atgtggcgca caaaaccagc attttcaacg gactagtagg
     4381 cctgataaga cacgtcaagc atcgcatcag gcagttgggc acggctgccg gatgcggcgt
     4441 gaacgcctta tccagcctac aaaaccagca atttcaacgg agttgtaggc ctgataagac
     4501 gcgtcaagcg tcgcatcagg cattttggct caaaagtatc aagccagtgc tttggtgatc
     4561 ttctcgtaca gatcgccaga gagattttcc agccctttca actgttccag cgccgcgcgc
     4621 attttctcct gacgtttggc atcataacgt ttcaggcgaa tcagcggttc aatcagacgt
     4681 gaagctacct gcgggttacg gctgttgagg tcggtaagca tttccaccag gaactggtaa
     4741 ccgctgccat cttcggcatg gaacgctgcc gggttgctgc ccgcaaacgc accaatcaac
     4801 gaacgaatac ggttcgggtt gctcatggta aatgagcgat gctgcaacag accacgcacc
     4861 gtctccagca cattcgccgc cgggctggtg gcttgcagga taaaccattt atccatcacc
     4921 agaccgtcct gatgccactt atcgtcgtac tcctgcatca gcgcgtcacg gcaaggcagc
     4981 tgtgcagcaa ccgccgcaga aagcgctgcc agcgcatcgg tcatattgtt tgcttcatgg
     5041 tattgcttgc tcaccagcac gtcagccaga tgcgtttcac caaaagcaag gaagcgcagg
     5101 caggcattac gcagagtgcg ttttgcaata tcttcatgct caacacggta ctcgctctgg
     5161 taattcgcgt tgtaaatagc cagcagctca tccgccagtt cagtcgccag agtacgagtg
     5221 agtgcttcgc gtacttcggc aatagcaatc gggtcgatga tatcgaacag ttcagccatt
     5281 tcattgacag aaggcagcgt caggatttct gccgccagcg ccggatcaat cttctcatcg
     5341 agcagcaccg cgcggaaagc gtcagccaca tgtaccggca gagacagcgg ctgcccttgc
     5401 tgatgacggg cgacgttcag cttgatgtag gttgccagca aactttgcgc cgcatcccaa
     5461 cgggagaaat cattacgcgc atgacgcatc aggaaggtca gttgctgatc gctccactta
     5521 tattccagtt tcactggcgc agagaattcg cacagcagcg caggcaccgg ctggaagtag
     5581 acattatcaa aaacaaaggt ctgttccgcc tgggtgacgt tcagcacgga attcaccgga
     5641 tgaccgcctt tctgcaacgg gatcactttg ccttcgttgt catacaattc gatggcaaat
     5701 ggaatatgca gcggctgttt ttctgcctga tccggcgtgg ctggcgtgcg ctggctgatg
     5761 gtcagggtgt actgctcggt ttccggattg tagtcgtctt tgacggtcac aatcggcgtg
     5821 ccggactggc tgtaccaacg gcggaaatgg gagagatcga cattcgacgc atcttccatc
     5881 gcctgcacaa agtcgtcgca ggttgccgcg ctgccatcat ggcgctcgaa atatagttgc
     5941 atccctttct ggaagttttc ttcgcccagc agggtgtgga tcatgcgaat cacttccgcg
     6001 cccttctcgt aaacggtcag ggtgtagaag ttgttcatct caatgaccat atccgggcgg
     6061 atcgggtgcg ccatcgggct ggcgtcttcc gcaaactgca atccgcgcat ggtgcgcaca
     6121 ttattgatac ggtttaccgc gcgggaacca aggtcagagc tgaattcctg atcgcggaag
     6181 acggttaaac cttctttcag gctgagctgg aaccagtcgc ggcaggtcac tcggttaccg
     6241 gtccagttat ggaaatattc atggccgata acgcgttcaa tatcgagata atctttgtcg
     6301 gtggcggtgt cggtgcgggc caacacatat ttggagttaa agatattcag acccttattc
     6361 tccattgcgc ccatattgaa gaaatccacc gcgacgatca tatagatgtc gaggtcatac
     6421 tccaggccaa agcgttcttc atcccatttc atggagtttt tcagcgaggt catcgcccac
     6481 ggcgcgcgat caaggttgcc gcgatcgacg tacagctcca gtgctacttc gcgaccagaa
     6541 cgcgtggtaa aggtatcgcg cagtacatca aagtcgcctg ccaccagcgc aaacaggtag
     6601 cacggtttcg ggaacgggtc ctgccactgt acccaatggc gtccgttttc cagttcgcct
     6661 tgcgcaacgc ggttaccatt ggaaagcagg aagggatatt tgattttatc ggcaataatt
     6721 ttggtggtaa aacgcgccag cacgtccggg cggtcgagat aatacgtaat atggcggaaa
     6781 ccttcggctt cacactgggt gcaaagcgca tcgcctgact gataaagccc ttccagcgcc
     6841 gtattcgccg ccgggcttat ttcattaatg atcttaagcg taaaacgctc cggcaaatta
     6901 ctgatgacca gtgcgccctc ttcttctttc caggcggtcc acggctcatc attaatatga
     6961 acagaaacca gtttgaggtc ttcgccgttg agacgaagcg gagcatctga tgcaccatga
     7021 cggacagcct ggctgaccgc ggtaacgacc gtcttttgcg cgtcgaggtc aaaggtcaag
     7081 tcaatatcag taatctggta atccggcgca cgataatcgt gacggtattt ggcttgtggc
     7141 tgttgagtca taaataacct ttagcatctt ttatagagtc tggtgttcag tctattcctg
     7201 ttgcgtaaat cgcgctatgc agaatcttca tcttttcagg tacaaacgcc tttattgcta
     7261 catttttata acatacaccg cgtaatgcca tcgaccagaa aggtggcata tggtgtgatc
     7321 ggggttcaat aaattgcgaa acaaggtata ctccagcagt tcctgaagat gtttattgta
     7381 ctaaacgctc ctgtacgagg acgctactgc gcacctatga cacaattcgc ttctcctgtt
     7441 ctgcactcgt tgctggatac agatgcttat aagttgcata tgcagcaagc cgtgtttcat
     7501 cactattacg atgtgcatgt cgcggcggag tttcgttgcc gaggtgacga tctgctgggt
     7561 atttatgccg atgctattcg tgaacagatt caggcgatgc agcacctgcg cctgcaggat
     7621 gatgaatatc agtggctctc tgctctgcct ttctttaagg ccgactatct taactggtta
     7681 cgcgagttcc gctttaaccc ggaacaagtc accgtatcca acgataatgg caagctggat
     7741 attcgtttaa gcggcccgtg gcgtgaagtc atcctctggg aagttccttt gctggcggtt
     7801 atcagtgaaa tggtacatcg ctatcgctca ccgcaggccg acgttgcgca agccctcgac
     7861 acgctggaaa gcaaattagt cgacttctcg gcgttaaccg ccggccttga tatgtcgcgc
     7921 ttccatctta tggattttgg cacccgtcgc cgtttttctc gcgaagtaca agaaaccatc
     7981 gttaagcgtc tgcaacagga atcctggttc gtgggcacca gcaactacga tctggcgcgt
     8041 cggctttccc tcacgccgat gggaacacag gcacacgaat ggttccaggc acatcagcaa
     8101 atcagcccgg atctagccaa cagccagcga gctgcacttg ctgcctggct ggaagaatat
     8161 cccgaccaac ttggcattgc attaaccgac tgcatcacta tggatgcttt cctgcgtgat
     8221 ttcggtgtcg agttcgccag tcgctatcag ggcctgcgtc atgactctgg cgacccggtt
     8281 gaatggggtg aaaaagccat tgtacattat gaaaagctgg gaattgatcc acagagtaaa
     8341 acgctggttt tctctgacaa tctggattta cgcaaagcgg ttgagctata ccgccacttc
     8401 tcttcccgcg tgcaattaag ttttggtatt gggactcgcc tgacctgcga tatcccccag
     8461 gtaaaacccc tgaatattgt gattaagttg gttgagtgta acggtaaacc ggtggcgaaa
     8521 ctttctgaca gccctggcaa aactatctgc cacgataaag cgtttgttcg ggcgctgcgc
     8581 aaagcgttcg accttccgca tattaaaaaa gccagttaat atcatcaggg agctaatcgg
     8641 ctcccttttt ttacctttaa ttccgaaatc tttcgctgca tttgcgaatt ctgcttgtct
     8701 gattgcagat gccaggtaac ataggtatcc ccccatttac gggtggacaa gtgtttattt
     8761 tttccgacta ttaacagaga gaatattatg agcgttgtgc ctgtagccga cgtactccag
     8821 ggccgcgtag ccgttgacag cgaagtcacc gtacgcggat gggtacgtac ccgccgagat
     8881 tcaaaagctg gcatctcctt cctcgccgtt tatgacggtt cctgctttga tcctgtacag
     8941 gctgtcatca ataattctct gcccaattac aatgaagacg tcctgcgtct gaccaccggc
     9001 tgctcggtca ttgtgacggg taaagtcgtg gcgtcgccgg gccaggggca acaatttgaa
     9061 attcaggcca gcaaagttga agttgctggt tgggttgaag atccagacac ttacccgatg
     9121 gcggcaaaac gccacagcat tgagtatctg cgtgaagtcg ctcacctgcg tccgcgcaca
     9181 aacctgattg gtgccgtcgc gcgcgttcgc catacgctgg cgcaggcgct gcatcgcttc
     9241 tttaacgagc agggattctt ctgggtttca acgccactga ttaccgcatc tgataccgaa
     9301 ggtgcaggcg aaatgttccg cgtttctacg ctggatctgg aaaacctgcc gcgtaacgat
     9361 cagggcaaag tggatttcga caaagacttc tttggtaaag agtctttcct gaccgtatct
     9421 ggccagttga acggcgaaac ctacgcttgc gcattatcca aaatttatac cttcggcccg
     9481 actttccgtg ctgaaaactc caacaccagc cgtcacctgg cggaattctg gatgctggag
     9541 ccggaagtgg cgtttgctaa cctgaacgat atcgcgggtc tggctgaagc catgctgaaa
     9601 tatgtcttca aagcggttct cgaagaacgc gcagacgaca tgaaattctt cgctgaacgc
     9661 gtagataaag atgccgtttc acgtctggaa cgcttcattg aggccgattt cgcgcaggtg
     9721 gattacaccg acgcagtgac cattctcgaa aactgcggca ggaagtttga aaacccggtt
     9781 tactggggcg tcgatctctc ttctgagcat gagcgttatc tggcggaaga acactttaaa
     9841 gcaccggtag tggttaaaaa ctatccgaaa gatattaaag cgttctatat gcgcctcaac
     9901 gaagacggta aaaccgttgc ggctatggac gttctggctc cgggcatcgg tgagatcatt
     9961 ggtggctccc agcgtgaaga acgtctggac gttctggacg agcgtatgct ggaaatgggc
    10021 ctgaataaag aagattactg gtggtatcgc gatctgcgtc gctacggtac tgttccgcat
    10081 tcaggtttcg gtcttggttt tgaacgcctg attgcttacg taactggcgt gcaaaacgtg
    10141 cgtgatgtga ttccgttccc acgtactccg cgcaacgcca gcttctgatt ttcttcctca
    10201 aagagccagc gcctgctggc tcttttttat cctttctgtc aagttatctg tttgttaagt
    10261 caagcaatct atttgcaacc ccgccataac gatcatcctg ttacggaata ttacattgca
    10321 acatttacgc acaaaaaata acccgcattc ttattgcgga ttagtttttt cttagctgat
    10381 agcacaattt tcatactatt ttttggcatt ctggatgtct gaaagaggat tttgtgccag
    10441 gtcgataaag tttccatcag aaacaaaatt tccgtttagt taatttaa
//
LOCUS       DAZFXT010000155        10195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.155, whole genome shotgun sequence.
ACCESSION   DAZFXT010000155 DAZFXT010000000
VERSION     DAZFXT010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.155"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            267..770
                     /gene="ftnB"
                     /locus_tag="ACHKW0_004232"
     CDS             267..770
                     /gene="ftnB"
                     /locus_tag="ACHKW0_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HFO7476965.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(849..1100)
                     /gene="yecJ"
                     /locus_tag="ACHKW0_004233"
     CDS             complement(849..1100)
                     /gene="yecJ"
                     /locus_tag="ACHKW0_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 family protein YecJ"
                     /protein_id="HFO7476966.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1214..1300)
                     /gene="azuC"
                     /locus_tag="ACHKW0_004234"
     CDS             complement(1214..1300)
                     /gene="azuC"
                     /locus_tag="ACHKW0_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HFO7476967.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1563..1886
                     /gene="yecR"
                     /locus_tag="ACHKW0_004235"
     CDS             1563..1886
                     /gene="yecR"
                     /locus_tag="ACHKW0_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HFO7476968.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2057..2554
                     /gene="ftnA"
                     /locus_tag="ACHKW0_004236"
     CDS             2057..2554
                     /gene="ftnA"
                     /locus_tag="ACHKW0_004236"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HFO7476969.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2592..2831)
                     /gene="yecH"
                     /locus_tag="ACHKW0_004237"
     CDS             complement(2592..2831)
                     /gene="yecH"
                     /locus_tag="ACHKW0_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family metal-binding protein"
                     /protein_id="HFO7476970.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            3022..4233
                     /gene="tyrP"
                     /locus_tag="ACHKW0_004238"
     CDS             3022..4233
                     /gene="tyrP"
                     /locus_tag="ACHKW0_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015173 - aromatic amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015801 - aromatic amino acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HFO7476971.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4295..4960)
                     /gene="yecA"
                     /locus_tag="ACHKW0_004239"
     CDS             complement(4295..4960)
                     /gene="yecA"
                     /locus_tag="ACHKW0_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HFO7476972.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5156..5242)
                     /locus_tag="ACHKW0_004240"
     tRNA            complement(5156..5242)
                     /locus_tag="ACHKW0_004240"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5206..5208),aa:Leu,seq:taa)
     gene            complement(5255..5328)
                     /locus_tag="ACHKW0_004241"
     tRNA            complement(5255..5328)
                     /locus_tag="ACHKW0_004241"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5294..5296),aa:Cys,seq:gca)
     gene            complement(5383..5458)
                     /locus_tag="ACHKW0_004242"
     tRNA            complement(5383..5458)
                     /locus_tag="ACHKW0_004242"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5423..5425),aa:Gly,seq:gcc)
     gene            complement(5610..6158)
                     /gene="pgsA"
                     /locus_tag="ACHKW0_004243"
     CDS             complement(5610..6158)
                     /gene="pgsA"
                     /locus_tag="ACHKW0_004243"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HFO7476973.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSASRADLLDQ"
     gene            complement(6215..8047)
                     /gene="uvrC"
                     /locus_tag="ACHKW0_004244"
     CDS             complement(6215..8047)
                     /gene="uvrC"
                     /locus_tag="ACHKW0_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HFO7476974.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGVDRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8044..8700)
                     /gene="uvrY"
                     /locus_tag="ACHKW0_004245"
     CDS             complement(8044..8700)
                     /gene="uvrY"
                     /locus_tag="ACHKW0_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HFO7476975.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8842..8913
                     /locus_tag="ACHKW0_004246"
                     /pseudo
     CDS             8842..8913
                     /locus_tag="ACHKW0_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302039.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            8996..9172
                     /gene="yecU"
                     /locus_tag="ACHKW0_004247"
     CDS             8996..9172
                     /gene="yecU"
                     /locus_tag="ACHKW0_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YecU"
                     /protein_id="HFO7476976.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9159..9383
                     /gene="yecF"
                     /locus_tag="ACHKW0_004248"
     CDS             9159..9383
                     /gene="yecF"
                     /locus_tag="ACHKW0_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein YecF"
                     /protein_id="HFO7476977.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9450..10172)
                     /gene="sdiA"
                     /locus_tag="ACHKW0_004249"
     CDS             complement(9450..10172)
                     /gene="sdiA"
                     /locus_tag="ACHKW0_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HFO7476978.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2574 a         2551 c         2341 g         2729 t
ORIGIN      
        1 ttaatatcaa caagatacaa aaagcgctat cattaaaatt cattgcagtt acattgattt
       61 catcaatgaa atgtaaaaat atataaactt gatgatttaa gcattttctt atacccgttc
      121 agacgttatt cttatttcag atcatcgtca gaattgactc cacgatcaca tttcggaccg
      181 gcagaaagga attattctgc aaacagtaat tatggtgttt tgatttatct tgcacctctc
      241 cacttctgga tataaggata ttaggtatgg caaccgctgg aatgcttctc aaactcaact
      301 ctcaaatgaa ccgcgagttt tacgcatcca atctctacct tcacctgagt aactggtgtt
      361 ctgaacagag tctgaacggc accgccactt tccttcgcgc ccaggcacag agtaatgtga
      421 cccaaatgat gcgcatgttt aactttatga agagtgtcgg cgctaccccc atcgttaaag
      481 ccattgatgt tcccggtgaa aaactgaact ctctggaaga actgttccaa aaaacgatgg
      541 aagaatacga gcaacgttct agtacgttgg cacagttagc cgatgaagcg aaagaactga
      601 atgatgattc aaccgtcaat ttcctgcgcg atctggaaaa agaacagcag catgatggtc
      661 tgttgctgca aaccattctt gatgaagtgc gcagtgcgaa acttgcgggt atgtgccctg
      721 tgcagaccga ccaacatgtt ctgaatgtcg tgtcacacca gctgcattga tcatcatcgg
      781 cgctaatgca ttgcgccgat gaaggttttg agaaaccgct gcctcatctg tttgaagcag
      841 cggttttttt aatgggattc accctgtggg gtaaacttga gttcaataag cgcgatggct
      901 ttttggattg cccgcatggt gaccgggtct gcggcggcgg gatggttagt aaagtcgata
      961 ttcttcagct gactggacat tttttcacga acttcaacgg gcgcgattac atcgagaaca
     1021 tccagaattt gtttgataac caactggcaa gcaaccacat cagaaaccag ttcctgatcg
     1081 gcattcagcg gctgggacat cgtaaactcc tgatagcatt ttgaaagccg ttatagtagc
     1141 ggcttcacat cttcagcgat agtcacatcc accgtcatca ggacacaaaa aacctgccgg
     1201 agcaggtttt ttattatcgg aacatattgc ctggcggtac gtctttgaac gtcttgcaat
     1261 agttattgaa catacttttc aggattttgc gcagtttcat cgcggcactc cgaccatttg
     1321 ttatacaggt gttattctct ttgcgcttat aatatgacaa ccatcacaaa aatcaatctt
     1381 tatgtgatac aaatcacata aacacccctt taatgttata aaaatgataa tcaaaaaaca
     1441 gcccccctat ttctgacacc tacagatggc aagaaatagc gcctgccagg cgtcttttcc
     1501 ggccattgtc gcagcgctgt aacgcgtaaa atagtgcttt ctcttactct tctggctgga
     1561 ccatgagact tctgattctg actctttcat taataacgct ggcggggtgt acggttactc
     1621 gccaggccca tgtgagcgaa gttgacgcgg caacaggtat tgtacggttg gtttatgacc
     1681 aggctttttt gcagcatgcc cataccgatc gttatgtcag tcgcgggatc gccgatcgcg
     1741 catgccagca ggaaggctat acccacgcgg tcccctttgg tcagccagta ggcaactgta
     1801 gcctttttgc gggttcctta tgtttgaata cggaattcac tttgtcatat cagtgccatc
     1861 attccgcctt ccctgtcttt ctctaaatat tttaatcagc gaggggatct tcgctgatta
     1921 aagaaatagc ggtaatgcgt tttattccca ttcgtatttt taataattga agtttatatt
     1981 ttaccttttg caaataataa aataacaaat tatagtgacg ccacagcaac aaatataacc
     2041 tttgtggagc actatcatgc tgaaaccaga aatgattgaa aaacttaatg agcagatgaa
     2101 cctggaactg tactcttcac tgctttatca gcaaatgagc gcctggtgca gctatcatac
     2161 cttcgaaggt gctgccgcgt tcctgcgccg tcacgcccag gaagagatga cgcatatgca
     2221 gcgtctgttt gattacctga ctgataccgg caatttaccg cgtattaata ccgttgaatc
     2281 tccgtttgct gaatattcct cacttgatga attattccag gaaacctata aacacgaaca
     2341 attaatcacg cagaaaatta acgaactggc tcatgctgca atgaccaatc aggactaccc
     2401 aacatttaat ttcctgcaat ggtatgtttc tgagcagcat gaagaagaga aactgttcaa
     2461 atcgattatt gataaattaa gcctggcagg caaaagcggc gaaggtctgt attttatcga
     2521 caaagaactc tctaccctcg acacacaaaa ctaatgctct caggcggcag gttttctgcc
     2581 gccgtttcaa atcagtgacg gcaaatctta ctctgatcgg tcgaaaaacc ttcttctgaa
     2641 ggtataaatt tgccttttgc tgccagaaac gctaccagct ctcccgcagt catcccttct
     2701 gccgagcagg tgtgaaaacg tgcctgttca ccaaaacgcg ctttaattgc tgcttccaga
     2761 ctggcatgcg tatattgctc gcctgattca atcatcatat ttaacacttc atgaccgtga
     2821 atagagtcca tcgtccctcc tcaaaaaaag cctagcgtag cgattgccgc ttatgaagac
     2881 tttgcgccag cgcaggactg aatgcttttt attgtacatt tatatttaca ccatatgtaa
     2941 cgtcggtttg acgaagcagc cgttatgcct taacctgcgc cgcagatatc actcataaag
     3001 atcgtcagga cagaagaaag cgtgaaaaac agaaccctgg gaagtgtttt tatcgtggcg
     3061 ggaaccacaa ttggcgcagg catgctggca atgccgctgg ctgcggccgg tgttggtttt
     3121 agcgttacgt taatcttgtt gattgggctt tgggcgttga tgtgctacac ggcgctatta
     3181 ctgctggagg tgtaccagca tgttccggca gataccggtc ttggcacgct ggcaaaacgc
     3241 tatctgggac gctacggtca atggctgacg ggcttcagta tgatgttctt aatgtatgct
     3301 ctgactgcgg catacatcag cggtgccggt gaattgttgg cctccagcat cagcgactgg
     3361 acaggtattt caatgtcggc aaccgctggc gtgctgttgt tcacttttgt tgccggtggc
     3421 gtggtttgtg ttggaacatc gctggtcgat ttatttaacc gttttctgtt cagcgccaaa
     3481 attatttttc tggtggtgat gctggtattg ctgctaccgc atattcacaa agtgaatctt
     3541 ttaaccctgc cgttgcaaca ggggctggct ctgtcagcaa tcccggtgat ttttacctca
     3601 tttggttttc acggtagcgt gccgagtatt gtcagctata tggatggcaa cgttcgtaag
     3661 ctacgctggg tgtttataac cggtagtgcg atccccctgg tggcatatat tttctggcag
     3721 gtggcaacgc ttggcagcat tgattcaaca acctttatgg gattgctggc taatcatgct
     3781 ggattaaacg ggctgttaca ggcgttacgc gaaatggtgg cctctccgca tgttgagctg
     3841 gcagtgcatt tatttgctga tttagccctc gccacgtcat ttctcggcgt tgcgttaggc
     3901 ttatttgatt atctggctga tttatttcag cgttcaaata ccgttggtgg acggttgcaa
     3961 actggtgcaa ttacctttct gccgccgttg gcgtttgcac tgttttatcc acgaggattt
     4021 gtgatggcgc tgggttacgc cggtgtggcg ctggcggtac tggcattgat tatcccttcg
     4081 ctgttgacct ggcaaagcag aaagcacaat cctcaggcgg gttaccgggt gaaaggtggt
     4141 cgtccggcgc tggtggtggt gtttctctgt ggtattgctg tgattggcgt gcaatttttg
     4201 attgcggcag ggttgttacc agaagtgggg tgatcagata gcctcaaatt ccttattggg
     4261 tgccagaatt aacgctgaca cccaatttga cctcttaatg caggcagcac tgcttaaatt
     4321 tcttaccact accgcacggg caaggatcgt tacgccccgg tttctcttct gctttgatcg
     4381 gttgctgaac agctttttcc tgcggatgcg ccatccagta cgcatgtaga tcaagcgccg
     4441 ccagtcgaat ggcatctacg ctctcttcaa acgcttctgg cgacattttc tctacccgct
     4501 cgaagttttc ctcagtaccg tgcagcgcaa tcgcctccag cgctggtttt aacgaatcgg
     4561 gcaacgttga ccagtcagaa agtgccacgc cccgcatata gccaaagcac cactcctcaa
     4621 caatcgtcag ctcgctgcca tcaacttctc gcaagccgaa taacggctca aactgctccg
     4681 ggaattcgtt cagacgctct gcggtatcgg ccatatgttg aaaagccaga ttcataaagc
     4741 gcgtcatctc tttctctgac gcccagcgcg gcacatagtc agccccaccc cacacggcaa
     4801 ccagccactg ttccggttca atctcttgcg gagaactcaa caccgccgtc aataaaccgt
     4861 ccagctccgc cacatcaagg atggcgtggt cagtgttgta tttggtcaga atatcgtcca
     4921 gccattccaa ctcactttcg tttaacggtc ccgttttcat acgcttttcc ttgtggatct
     4981 caactcgcca gcacctatct tacatgccgg tccgtatcag agatactttt tgagtggctt
     5041 tgctggtgat taaaaattag ggagggtgta acgacaagtt gcaggcacaa aaaaaccacc
     5101 cgaaggtggt ttcacgacac tgcttattgc tttgatttta ttcttatctt tcccatggta
     5161 cccggagcgg gacttgaacc cgcacagcgc gaacgccgag ggattttaaa tcccttgtgt
     5221 ctaccgattc caccatccgg gctcgggaag aaagtggagg cgcgttccgg agtcgaaccg
     5281 gactagacgg atttgcaatc cgctacataa ccgctttgtt aacgcgccaa attcttcagg
     5341 cctttcagcc agacatccgc ttgacgccga tgtcttttaa actggagcgg gaaacgagac
     5401 tcgaactcgc gaccccgacc ttggcaaggt cgtgctctac caactgagct attcccgcat
     5461 tcatcaagca atcagttaat cacttgattt tattatcgtc tggcaatcag tgccgccgtt
     5521 cgatgcgttg cattctactt acctggcgcg atgagtcaac gatatttttc accacttttg
     5581 atcgtttgct gaaaattacg ccgaaacgat cactgatcaa gcaaatctgc acgcgaagcg
     5641 ctcaaatatt gcaacattga ccacagagtc agtaccgcag ccacaaagaa aagtgcaata
     5701 ccggcgtact caacccaaat gttcggacgc cacagcagcc aggccaacgc caccatctgg
     5761 gcagtggttt tcactttccc aatccaggag acagccacgc tactgcgttt acccaactcc
     5821 gccatccatt cgcgtagcgc agaaataata atttcacggg cgatcatcgt tgccgccggt
     5881 agggtcaccc accagctgtg ataatgctcg gttaccagca ccatggcgat agccacgaga
     5941 actttatctg ctacagggtc aaggaaagca ccaaaccggg tactctggtt ccagcggcgt
     6001 gccagaaaac catcgaacca gtcagtcacc gccgcgacgc agaaaatgag cgcggcggca
     6061 aacggcgacc aggtgacagg cagataaaag accaatacaa agaatgggat aaggatgaca
     6121 cggaacagtg taagcaacgt agggatatta aattgcataa tgacgggtaa ctatctgttg
     6181 tcagtaagat tacccctatg ttgctacaga gacatcaatg tttcaacgac cagaagatct
     6241 tttctgccag accttgcgaa atacccggca cttttgcaat ttcctcgacg ctggcgttac
     6301 gtaaaccttg caaaccgccc atatatttca acaacatttg ccgacgtttt ggcccgacgc
     6361 cttcaatggt ttccagggag ctggtatttt tgaccttcgc ccgtttttta cggtgcccgc
     6421 caatcgcgtg atcgtgtgat tcatcgcgaa tatgctggat aacatgcagc gcgggagaat
     6481 ctggcggcaa actgaatccc tcaccttccg gctcaaagaa cagtgtttcc agcccagcct
     6541 tacgatctac tcctttggca acgccaagta gcagtggatg atttttatcc catgagacat
     6601 ccagttcggc gaagacattt ttcgcctgcg caagctggcc tttgccgccg tcgataagga
     6661 tcacatccgg gatcttactg tcgtcaatgg ctttaccata acgccgacgc agcacctgat
     6721 tcatcgccgc ataatcatcg cccggcgtga tgcctgtaat gttatagcgc cgatactccg
     6781 cacgcagcgg gccgttagca tcaaacacca cacaggaagc gacggtttgt tcgcccatgg
     6841 tatggctgat gtcaaagcac tccatccgct tcacttccgg caatttcaac acgctggcaa
     6901 gcgcggtcag tcgctggtga acggtagatt gctgcgaaag tttgctggtt aaggccgtcg
     6961 ccgcattggt gcgcgcgagt ttcagataac gcgccctatc gccgcgaggt ttggtttgaa
     7021 cattaatctt gcgtcccgcc agttctgaaa gggaatcggc gagcagcgtt ttatcgctaa
     7081 gattaaaatc gagcaggatc tcacccggta aggtgcgcat ctggctgcct tgtaaataga
     7141 actggcctac gaaggtttct accacctcgc tcagttccgt accgccaggc actttcggga
     7201 aatagctgcg gctgccgagc actttgccct gacgaatgaa caatacgtgg acacaagcca
     7261 tgcccgcatc gaacgccaca ccaataacgt cgaggtcgtc gccggtattg gaaacgaatt
     7321 gtttttcggt gacgcgtcgc accgcctgaa tttggtcgcg aatacgtgca gcttcttcaa
     7381 actccagatt ctggctggca gtttccatac gactaatgag ttgcgtaagc acctgatcat
     7441 ctttgccaga caaaaacagg cgcacatact cgacctgctg agcgtattct tcttcactca
     7501 ccagtccttc aacgcacggt cccagacagc gccctatctg gtattgcaga cacggacgcg
     7561 agcgattgcg ataaacacta ttttcgcact ggcgaatggg gaaaatcttt tgcagtagcg
     7621 ccagtgtttc acgtacggca tagccattcg ggaacgggcc gaaatattca cctttggcat
     7681 gcttcgcacc acgatgcatc gccagacgcg ggtgggtatc accactcagg aagataaaag
     7741 gatatgattt atcatcgcgt agcaaaacgt tgtaacgcgg ctgatagagt ttgatgtagt
     7801 tgtgttccag caacagcgct tcggtttctg tgtgagtaac cgttacatca atttgctgga
     7861 tctgggcgac cagcgcttcg gttttgcgcg aagcgaggtt gctacggaaa tagctggaaa
     7921 gccgtttttt caggtctttc gctttgccga catagataac cgtaccacca gcatcgtaca
     7981 tgcgataaac gcctggctgg ctggttacgg tttttaaaaa cgcttttgcg tcaaactgat
     8041 cactcactga cttgataatg tctccgcatt acacagacca tggcgaattg ccaggtgagt
     8101 cagctcaaca tcgccatgaa tgtttagttt actgaacata cgatagcggt agctgttcac
     8161 cgttttcgga ctgagattga gctgttctga gatctcattg accttctggc ccttggtgat
     8221 catcagcata atctgcaatt cacgttcaga caaactggca aatgggcttt ctgttttttc
     8281 tggttcgatc tggcttaacg ccatttgttg agcgatgtca gaagcaatgt aacgctgccc
     8341 tgaatagaca gaacgaatcg cactcacaac ttcctgcgga gccgcgcctt tgctgaggta
     8401 gcccgcagca ccggcctgca tgactttcgc tggtaaaggg ttttctgtat ggacggtaag
     8461 catgatgatt ttgacatcag ctgtggaacg cgcgatttta cgcgtcgcct caagaccgcc
     8521 aatgcccggc atactcatgt ccattagcac cacgtcaacg gcatttgtcc ggcaccactt
     8581 aacggcgtct tcaccgcacg atgcctcacc gacgactttt atacccttta tatcttccag
     8641 aatgcgtcgt atccctgcgc gcaccagttc gtggtcatca acaagtagaa cgttgatcaa
     8701 aggaatatct ccagaaatag ggataacgct actgatagtt agtcattcgt atattagcgg
     8761 tttttattgc aactttgaaa cgttaaaaat ggtgggtatt cgatttttct ctcgtttttg
     8821 tcaattcgat tgtccacagt tatggtcacg cccgtcacca acggcttacc ggatcgtttc
     8881 acagtaagcc ctacgatgaa atgtgacaaa aattgacttt attcagcaaa aatgaaaatc
     8941 agcccgctga atatgtaaca ttaattaacc cgaacaatac gggcaaaaaa cattcatgat
     9001 aaaaatattt attggtcatt atattaacgt attttatagc actgccgata tcacgctcaa
     9061 aaaacaacca ctgctatttt tagcaaagct tatggtatac tccgccgcct taacattttt
     9121 caccgcaaat tttcattgca acatgacgag gaaaataaat gagtacgcct gatttttcta
     9181 ctgccgagaa taatcaagaa ctggcaaatg aagtctcctg cctgaaagcg atgctgacgc
     9241 tgatgctgca ggcgatggga caagctgacg cgggccgcgt gatgttaaag atggaaaaac
     9301 agcttgcgct gatcgaagac gaaacccagg ctgcagtatt ttccaaaacg gttaagcaaa
     9361 ttaaacaagc ctaccgtcag taatataaaa ccggctgaga gcgtgccttt cagccggttt
     9421 ttgcatctgg cacgcagaaa gaaaagcgat caaattaagc cagtagcggc cgcgtaacag
     9481 gccacctggg tcttatttgg tgcattgatt tttttctgca tgtttttctg atggaaattg
     9541 accgtattct cagagattga caaaatcatc gctatctctg ctgatgtctt cccttccgcc
     9601 gtccatttca gaatttcttt ttcgcgcttg ctgaaattca tctctggcgt catcactatt
     9661 tcatcattta aacgcatcag agccatcaga ctttcgcgca ccagtaactg catttttaat
     9721 tgcagttcat cactaagaat gggtatttcg cgcgtgctgc aacgggaaaa ggacaaaaag
     9781 cccagcgccc gatttggcag cattaaatac tgagtgacac cgcggcgtaa accatgtgcg
     9841 cgcgcggctt cccataacgg ctgtgcttcg ctgaataagt catcattcca cattaaatgg
     9901 ccctgactaa agttttcagg gttcagcacc ggatcaattg cgagaaagtt ttttgcctga
     9961 taataactaa cccacgcctc agggtaattg gtgtagaaag ccactttagg tcgcgtgaat
    10021 ggtaccgggt ggcgaacaca taacgaatag taatcgtact ccagctgctg agcctgaagc
    10081 tctatttcat ggtagacctc ttctgcggcc tccatcctct gaaaacgcaa cagcatcgtg
    10141 cgacgccagc tgaaaaaatc cgtatcctgc atagtaaacc gcaacgcccc tgaga
//
LOCUS       DAZFXT010000156        10132 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.156, whole genome shotgun sequence.
ACCESSION   DAZFXT010000156 DAZFXT010000000
VERSION     DAZFXT010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.156"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..749
                     /gene="yfdQ"
                     /locus_tag="ACHKW0_004250"
     CDS             <1..749
                     /gene="yfdQ"
                     /locus_tag="ACHKW0_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO7476979.1"
                     /translation="SKIGADAVALPENFRIHDLEKFNLNRFRFRGALSTASIDDFTRY
                     SKDLADEGTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKTAPFSALLSVNGE
                     RNSQKSLAEWIEDWADYLVGFDANGDAIQATKAAAAVRKITIEANQTADFEDNDFSGK
                     RSLMESVEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVLVLRIIQLEAV
                     QEEMANEFRDLLVEKFKDSKVETFIGTFTA"
     gene            877..1413
                     /gene="yfdR"
                     /locus_tag="ACHKW0_004251"
     CDS             877..1413
                     /gene="yfdR"
                     /locus_tag="ACHKW0_004251"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7476980.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            1404..1766
                     /locus_tag="ACHKW0_004252"
     CDS             1404..1766
                     /locus_tag="ACHKW0_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476981.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLLRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            1766..2071
                     /gene="yfdT"
                     /locus_tag="ACHKW0_004253"
     CDS             1766..2071
                     /gene="yfdT"
                     /locus_tag="ACHKW0_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdT"
                     /protein_id="HFO7476982.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMSQQEVK"
     gene            1987..2166
                     /locus_tag="ACHKW0_004254"
                     /pseudo
     CDS             1987..2166
                     /locus_tag="ACHKW0_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518805.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            2298..3461
                     /gene="intD"
                     /locus_tag="ACHKW0_004255"
     CDS             2298..3461
                     /gene="intD"
                     /locus_tag="ACHKW0_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HFO7476983.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(3477..3553)
                     /locus_tag="ACHKW0_004256"
     tRNA            complement(3477..3553)
                     /locus_tag="ACHKW0_004256"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3517..3519),aa:Arg,seq:tct)
     gene            3796..4428
                     /gene="fimZ"
                     /locus_tag="ACHKW0_004257"
     CDS             3796..4428
                     /gene="fimZ"
                     /locus_tag="ACHKW0_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HFO7476984.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVELIDYAKLYELI"
     gene            complement(4431..4946)
                     /gene="sfmF"
                     /locus_tag="ACHKW0_004258"
     CDS             complement(4431..4946)
                     /gene="sfmF"
                     /locus_tag="ACHKW0_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HFO7476985.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     KADAIFMINYN"
     gene            complement(4957..5964)
                     /gene="fimH"
                     /locus_tag="ACHKW0_004259"
     CDS             complement(4957..5964)
                     /gene="fimH"
                     /locus_tag="ACHKW0_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HFO7476986.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYILMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            complement(5977..8585)
                     /gene="sfmD"
                     /locus_tag="ACHKW0_004260"
                     /pseudo
     CDS             complement(5977..8585)
                     /gene="sfmD"
                     /locus_tag="ACHKW0_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            complement(8616..9308)
                     /gene="fimC"
                     /locus_tag="ACHKW0_004261"
     CDS             complement(8616..9308)
                     /gene="fimC"
                     /locus_tag="ACHKW0_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HFO7476987.1"
                     /translation="MMTKIKLLMLIIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(9528..10070)
                     /gene="fimA"
                     /locus_tag="ACHKW0_004262"
     CDS             complement(9528..10070)
                     /gene="fimA"
                     /locus_tag="ACHKW0_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HFO7476988.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
BASE COUNT         2916 a         2332 c         2286 g         2598 t
ORIGIN      
        1 tcagtaagat tggtgccgat gctgtggcat tgcctgagaa tttccgcatt catgatctgg
       61 aaaaatttaa tttaaatcgc ttccgtttcc gtggtgcact ttccactgcc agcatcgatg
      121 attttacccg ttattctaaa gatcttgcag atgaaggcac ccgctgcttt atcgatgccg
      181 ataatatgcg tgccgtcagt gtgcttaacc tgggtactat tgatgagcca ggtcacgcag
      241 ataacactgc cactctcaaa ctgaaaaaga cagcaccgtt ctctgctctg ttgtctgtta
      301 acggcgagcg taactcccag aagtcactgg cagaatggat cgaagactgg gccgactacc
      361 ttgtgggctt tgatgctaat ggtgacgcca ttcaagcaac aaaagcggct gcggcagtcc
      421 gtaaaatcac gattgaagca aaccagaccg ctgattttga agataatgac ttcagcggca
      481 aacgctccct gatggagtct gtcgaagcga agaccaaaga cattatgcca gtggcatttg
      541 aatttaaatg cgttccgttt gaaggtctga aagaacgtcc gtttaaatta cgcctcagca
      601 ttatcactgg cgatcgtcct gtactggttc tgcgcattat tcagctggaa gcggtgcagg
      661 aagaaatggc taacgaattt cgtgatctgc ttgttgagaa attcaaagac agcaaagtag
      721 aaacctttat tggtactttc accgcctgat ttcattactg caaatgcccc tgcgggggca
      781 tttatggaaa cgtaattaac tcaataatca ccggatggtg agggcttcct tttaccagaa
      841 ttcagcgcgg tgcagtgcat atacgtggag aacaaaatgt catttattaa aactttttcc
      901 gggaagcatt tttattatga caagataaat aaagacgaca tcgatattaa cgatatcgcg
      961 gtttcccttt caaatatctg tcgctttgcc ggtcatcttt cgcacttcta cagcgtcgcc
     1021 caacatgcgg ttctttgcag ccagctggtg ccgcaggaat ttgcttttga agcgttaatg
     1081 catgatgcaa cagaagcgta ttgccaggac attcccgcac cactgaaacg ccttcttcct
     1141 gactataaac ggatggaaga aaaaatagac gccgtaatcc gtgagaaata cgggttaccc
     1201 ccagttatga gtacacccgt gaaatatgcc gatctcatca tgctggcaac cgaacgccgc
     1261 gatctcgggc ttgatgatgg ctctttctgg cctgtactgg aaggcatccc ggcaacagaa
     1321 atgttcaacg tgattccact ggcaccgggt catgcctacg ggatgtttat ggaacgtttt
     1381 aacgatttat cggagttacg caaatgcgca tgaatgtttt cgaaatggaa gggtttcttc
     1441 gcgggaaatg tgtaccgcga gatctgaaag tgaacgaaac aaatgctgag tacctgttac
     1501 gtaaattcga cgcgcttgaa gctaaatgtg cggcactgga aaacaaaata ataccagtgt
     1561 cagctgaact gccaccagca aatgaaagtg ttctgttatt tgatgctaat ggagaaggct
     1621 ggctgattgg ctggcgttct ctctggtaca cctggggaca aaaagaaacc ggagaatggc
     1681 agtggacatt tcaggtcggg gaccttgaaa acgtcaatat cactcactgg gcagtaatgc
     1741 caaaagcacc ggaggctgga gcataatgac cacatttacc aataaagaac tgattaaaga
     1801 aatcaaagaa cgaatcagca gcctagaggt tcgagacgat attgagcgcc gtgcttatga
     1861 aatcgcactc gtatctctgg aagtagagcc agatgaacgc gaagcctatg aattattcat
     1921 ggaaaagcgt ttcggtgact tagtagatcg tcggagagca aaaaacggcg ataacgaata
     1981 catggcatgg gatatgactc tcggttggat cgtctggcag caacgagctg gtatccattt
     2041 ttcaacaatg tcacagcaag aggtgaaata atggagccat acagcctcac actcgatgag
     2101 gcctgtcatt ttctcaaaat atccagaccg actgccatta actggatacg cacaggccgc
     2161 ctacaggcaa cacgtaaaga tccaaccaag ccaaaatctc cttacctcac aacacggcaa
     2221 gcttgcattg cggcgcttca gtctccgctg catactgtcc aggtgagcgc gggtgatggc
     2281 ataacagagg aaagaaaatg tcactcttcc gcagaaatga aatatggtat gcctcgtatt
     2341 cgctcccggg cgggaaacga attaaggaat ctcttggcac aaaggacaag cggcaagctc
     2401 aggagttgca cgacaagcga aaagcagaac tctggcgagt agaaaagcta ggggatttac
     2461 ctgatgtcac ttttgaagag gcctgcctaa gatggcttga ggaaaaagct gataaaaaat
     2521 ctctcgattc agataaaagc cggattgagt tctggcttga acattttgag ggtataaggc
     2581 ttaaagatat ctcggaggca aagatttact ctgctgtaag cagaatgcat aacagaaaga
     2641 cgaaagaaat atggaaacag aaagttcagg ccgccatcag gaaaggtaaa gaaccgcctg
     2701 tttatgaacc aaagccagta tcaactcaga caaaggcaaa gcatcttgcc atgataaagg
     2761 ccattctccg tgctgcagaa cgcgactgga agtggctgga aaaagcgcct gtcatcaaga
     2821 taccagcggt cagaaacaag cgagtcagat ggctggaaaa ggaggaagca aaacgcctta
     2881 ttgatgagtg ccccgaacca ctgaaatctg tcgtcaagtt tgcgctggca actggtctga
     2941 gaaagtcgaa catcataaat ctggaatggc aacaaatcga catgcagcga cgagttgcct
     3001 gggtgaatcc agaagagagc aaatcaaacc gcgccattgg tgtggcgctg aacgataccg
     3061 cctgtaaagt gttgcgtgat caaataggca agcatcacaa atgggtgttt gtacatacca
     3121 aggcggctaa gcgagcagat ggaacatcaa cgcctgcggt caggaagatg cgcatcgaca
     3181 gcaagacatc atggctatca gcttgtcgtc gtgcaggaat tgaagatttc cgtttccatg
     3241 acctcagaca cacctgggca agctggctga tccagtcagg cgtcccatta tctgtgcttc
     3301 aggaaatggg cggatgggag tccatagaaa tggttcgtag gtatgctcac cttgcgccta
     3361 atcatttgac agagcatgca aggaaaatag acgacatttt tggtgataat gtcccaaata
     3421 tgtcccactg tggaattatg gaggatataa agaaggcgta actgattgaa ttgtaatggc
     3481 gcgccctgca ggattcgaac ctgcggccca cgacttagaa ggtcgttgct ctatccaact
     3541 gagctaaggg cgcgttgata ccgcaatgcg gtgtaatcgc gtgaattata cggtcaaccc
     3601 ttgctgagtc aatggctttt gatctggttg ctgaacaagt gaacgaccgc gtctgatttt
     3661 ctgatttatt tcgctatagc ggcaaacaaa cacacaccgc tgcgcgtctg aatcaagaaa
     3721 acccgtattt tcatgtatca aagtacaatt taccgcccta acggaaaatt gtccgctcct
     3781 atgagactgg taactatgaa accaacgtcg gtgatcatta tggatactca tcctatcatc
     3841 agaatgtcta ttgaagttct gttgcaaaaa aacagtgaat tgcagattgt cctgaaaacg
     3901 gatgattacc gcataaccat cgattatctc cgaacccgtc ctgttgattt aatcattatg
     3961 gatatagact tgcccggaac agacggtttt accttcctga aaaggatcaa acaaatccag
     4021 agcacagtga aagtgttatt tttatcatcg aaatcagaat gcttttatgc tggcagagcg
     4081 atacaagctg gtgctaacgg ttttgtcagt aaatgcaatg atcagaatga tatttttcat
     4141 gccgttcaga tgatcctctc cggatacacg ttttttccca gcgaaacgct taactatata
     4201 aaaagcaata aatgtagtac gaatagttca acggtcactg tgctatctaa tcgtgaagtg
     4261 accatattac gttatctggt tagcggatta tctaataaag aaattgccga taagttatta
     4321 cttagcaata aaacagttag tgcgcataaa tctaatattt atggcaagct aggtttgcat
     4381 tcaattgtag agcttatcga ctacgccaaa ttatacgaat taatataata ttaattataa
     4441 ttgatcataa atatcgcatc cgctttcgcc acacctggcc gaacaccgct ggctaacgct
     4501 cgataattgg caaaaaagtt tagtgtgaca ttgccatttg catctacttc ctcagtcggg
     4561 ctcgccgctc ccagtgcgag ccgggagcga tcgctattac gtaattcgat ggcgatggtt
     4621 tgcgccattg cgggatcatc cagagccagc aggttggtat cggatgccgg cgttcccgta
     4681 aataaaatcg caactgaacc cggaggacat ccctccagcc gcaggctaaa agggacgagt
     4741 gccgtggtat cgccagcgtt cagtaattgt gtcgtaggcc atctgcctaa atctaccgtc
     4801 ttatcaatat ccgcagtgtt tacggtacag gagaaatcaa caacgttacc gtgcaaattg
     4861 atattaatcg ttcccaaagg gtcagctgcc catccactgg aaatccacag taacccgcag
     4921 aaacagctaa agagtgctct tctcattctc cgtacctcat tgataatcga cgcgtaaata
     4981 ccccagcgcg ctaaacggcc cttcggtcgg tttttgaccg gtaatactga taggccaggc
     5041 gcgaagtgtg acattggctg ccgcagctgc atccagacgg aaaggaataa cgctattgag
     5101 atcgttaggc gtgatcggcg tatcgttctg atcggcgaca ataaaaccta aatcctgatt
     5161 gtccgacacc atcgcctgac cagaaacggc actggcttcc agacgcattg ttaaataagc
     5221 ctgcgcagca acattcgtac atttgaccgc aatgctcttg gtttgcggca tgacaccagc
     5281 aggtcgatta cccggccctg ccgcactaaa taacgatgcg ccgatatcac caaaatcaaa
     5341 ttcaacaatc tgcccggcat ttaattcgca gttttgcggt acttcaaccc ggccaccaaa
     5401 actaatggta taaacaggtg taaccaacgg atcgtaaggc gttgatgtca catacacggt
     5461 aaacatcacc tgtctgggga tctccaccat gttaataaag ggacgaataa cctttaaacg
     5521 aaatattaaa cgagaatcca tcaccccgaa tggaaggttt tgcgaaacgt taggatctgt
     5581 tcccatcaga atataatttt gcggggggaa ctgaatatcc atcacactat caaccagact
     5641 catcgcacct aatagataat catttaattg catatattta taattatcga tagtttcctg
     5701 aacaataaag ttggtgacat aactacggta tgtataattc accagcgtac cgggtggaca
     5761 aattgctgag acacctaccc agccggattt ttccggtaaa acaacaatct gccctggctg
     5821 gttattggtg ctattaaaga tatttgagag atcatagtgt tcatcttctg ctacgccagt
     5881 actatttgca caaacagtag cccaggatat gtttgccaga cacaaacatg ccatcgccaa
     5941 taataatctg cagattatct tcattgataa tttcctttat gagccaggat gtgtgcaaac
     6001 tgccgaaata acagtgacgg cttgctgtaa gctttgcttc ggcaatacat agtgcgcaat
     6061 gcagcgtgag ttcgcgccgt ctccccactg aaccagtaat tcaccagaca atggcgcacc
     6121 acttaaataa acttgcccgt catcacccac atactggtta ttccgatact gttttcccgt
     6181 accagtgatc caaacggcac cggttttccg ccctgggtaa cggtaatgag cgcccgcacg
     6241 cctatgcggg tatcaaaatt ggcacgaacc aacgcgcctt gcgtcggcac aacgctgcta
     6301 atattttttt caacatcgat ggaattcccc atcgtattgg tatcaagcgc gatacggtta
     6361 taccgataaa ccgtggcata cggcatcacc gcatagccgc gccagtcggt taaaatgcca
     6421 gtttgatttt caatgcgtac accgcctgcg ccaggcgctt taatcaaaac attggtatcc
     6481 cctaaaggct ggctcagcgt tatgccattt tcatgcccga ccacaccgcc agacagctgc
     6541 cagttaacgt catgttgatc gcggtcgtag ttatacccga cccccagcgt agcgtaagcg
     6601 gcctgccagt ttgccgtggc gctgcccgta tacccattat tcgaggtatc accctggctt
     6661 acgtgataac tcaggttgtg gccttccagt aaggtcccac ctacacctgc cagccagcta
     6721 ttttgcccgt tgctgttacg gttggcgtta aaggaggcat aagcgcggtc gagcgcattt
     6781 tcccgggtgt agcgacgttt ggtcagaaca ttgaaaggca ctgaaacatt aagtccgaca
     6841 atacgttcgt tatcggggat ccctacagat tcattccacg aaaatgagag tgaataactg
     6901 atgccaaccc agctgctggt ataccccacc tgataccagg tatctgaatc cgaagtattc
     6961 cagtattttt gatgggtacc agagatataa agcgagccaa agtcattaag tgattgtgaa
     7021 atatttaact gcaaacggtc tttacggcta aagcgtaaat tgtggtaatt cacgattatc
     7081 ggttcatccc gatgctcacc gtcgtaatcg taatcatatt cgtacccctc cattcgacga
     7141 tacgcaacat catcaagggt ataaaaacct tgtgtcgaat agcggtaacc cattaactga
     7201 aaattggtgc cgaaggtgtt catcgatttc gcatagagga agcgaatgga atccccctca
     7261 tgacgactgt cgtcggctaa ctgactgcgc gcatgggtca catccagcga cactgccccc
     7321 cagtttccga gattgcgccc cagtcctaat aaaaaggcgg tgtaattggc agataattgc
     7381 gtcccgccgt aggcagtaaa ttcctgtggt aaaccgccga gtgccgtgcc ctgaaaaaag
     7441 aaaggcgatg attgctgact attaccgcta cgaaaatcgc ccgccgtcag gtcaaatttg
     7501 aaacgccctt cgcgttgtaa aattggcact gttgaatacg ggatagtgta attctgctga
     7561 ttaccatcgc gctcgtcgat ggtgacgtcc agatcgccat ttgaagatgt cgggtgcaaa
     7621 tctgtaattt caaaagcgcc gggggaaaca tagctttgat agataataaa accattttgc
     7681 cgaatcgtta gctgggccgc cgtacgggca atcccacgta ccgttggggc aaacccttgc
     7741 tggctatcag gatacatatt atcggaagaa taaagccgta caccacgaaa tccaacacca
     7801 tcgaaaatat cacttcctgt attgccgtct cccattacca gttcactttt cagcggaata
     7861 atggcgcgct gtacccaggt gccaatatta ttccactgtt ctgaatgata tccatttccg
     7921 cgaaaatagt tccaggaacc attgttgcgt aaacgccacg ggccaatatt aatcccgctg
     7981 agctcactaa aaaaatagct atcgttacca ttacctctgt taccggtgaa attataattc
     8041 aggagtaaag caggaatacc ttcatcccac tcttcaggcg gaatgtaacc gtgagcgcta
     8101 ctcaacaacg ttatctgagg aatagtgatg ttcaggcgca tcgctgcaaa atcaaagtta
     8161 attgtggcat caggtacagc tttcagtaaa ttgatgcatt tattgttttg ctgctgggct
     8221 aactcgggaa atgcactgct attaaggcca attcgttcaa ggagtacctg gttaaaacag
     8281 ggcattaacc cacctgattt atcacctgta ttttctgttg tcgattcaaa tacgatatcc
     8341 tgtgaaccaa tgaactcatc attacgccag agatccaccc gatacactcc cgcaggttga
     8401 tgattgcctc tctcaaagcg cgataaatca gcaacggatg tgccattctc taataaaaat
     8461 gccgggttga aataactttc cgcatagctt aaaaaggaga gtaaaatggc aagagatgaa
     8521 taacaaattc cggccagaca ccaggtatac ctctgcggaa tatccgtagt agtgggtatt
     8581 ttcataatta gctctgatat tgtcagatga ttcggttagt ttaagttcac ttctctgacc
     8641 ggggttactg aaccataatc attaacggtc tgaaatttca gctttccctg cactcctgag
     8701 ggtaagggaa tttgtgaact aactctgggt gccgccatag cattccccaa cttttggctg
     8761 cccacttgca ggttaacgag ggtgacataa aaaggtgatg gattatgaac attgatatag
     8821 ttaccggatc gcgaaaactt gagtgtgtcc ggcgcttctg cgggtaattc ttgtaattga
     8881 attggacgga gaaataattt catgcgcgat aaaatcgcca gttgcaaaac attcctgccg
     8941 ttcaatgcat ttttatctac cgaagggatc gtcttaacat tcatccagaa cagagactca
     9001 cgatctgctg ccagcggtgg accggtgtaa ataatacgca aagtattttc gcttttgggt
     9061 tcactaacaa acagtggcgg tgtaatgatg aatgactttt cttttacacc gctgctgttt
     9121 tcaatccacg aattgaccag aaaacgctca ttggtatggc tatttctaat ccataccgca
     9181 gtctgtttag catcagcggg ataaataata cgcgtggcac ctaatgcgat ccctccggca
     9241 gcatgggcgc tggccgaaat gattaaataa aatataatga gcatcaataa ctttatttta
     9301 gtcatcatag ttaatacgcc ttgtgtattc ctgacggcaa aaataagaaa tataaagaaa
     9361 tgattgcttt ggtttcttac aacacatcat tcccttttca tttaataaag cggcatgata
     9421 caaagtgaac atgatttcga tttacattgc caccataccg cttcagacaa ccggtacatt
     9481 actttgttat aagacgtgat atataattca aaacaacgtg gttttgatta ttcgtatctc
     9541 ataataaaag tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc ggtgccttta
     9601 taacgtgcag aaaaatgaag aacgttggtt ccggggatca agttctggtt ggttgagaag
     9661 ctattcccat caggtttgag aattgcggat gtattatcaa gtatttcaat accgacaccc
     9721 gttgctgttg ttgtattggt actggatgca atggccaata aattatcatt gattgcatca
     9781 gcctgaccag aaaatgcaac ggcagcatta gcggcaacaa caggatcgca gtcgttcaac
     9841 tgaatggtga atggaattaa tgcagaggta ttaccaacag cattgaaaat atcggtacga
     9901 tattgaccga gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca ggcagcattc
     9961 accagttcgc cttcaaagtg aattgtaccg ccatctacaa cggcagcata actacccgta
    10021 gcggcgaata gtgcggcagc cagcgcagac gaaataaatc ttaatttcat atatattcct
    10081 tcaatctcat ttatcgactc cacatccgta tataaccgat tactttattt aa
//
LOCUS       DAZFXT010000157        10123 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.157, whole genome shotgun sequence.
ACCESSION   DAZFXT010000157 DAZFXT010000000
VERSION     DAZFXT010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.157"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            191..343
                     /gene="hokA"
                     /locus_tag="ACHKW0_004263"
     CDS             191..343
                     /gene="hokA"
                     /locus_tag="ACHKW0_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012540334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HFO7476989.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQGSYELAAFL
                     ACNLKE"
     gene            complement(332..391)
                     /locus_tag="ACHKW0_004264"
     CDS             complement(332..391)
                     /locus_tag="ACHKW0_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7476990.1"
                     /translation="MRNAVRKAGITSRRRLLLF"
     gene            complement(531..743)
                     /gene="cspA"
                     /locus_tag="ACHKW0_004265"
     CDS             complement(531..743)
                     /gene="cspA"
                     /locus_tag="ACHKW0_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HFO7476991.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1024..1314)
                     /gene="yiaG"
                     /locus_tag="ACHKW0_004266"
     CDS             complement(1024..1314)
                     /gene="yiaG"
                     /locus_tag="ACHKW0_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HFO7476992.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1748..2458
                     /gene="yiaF"
                     /locus_tag="ACHKW0_004267"
     CDS             1748..2458
                     /gene="yiaF"
                     /locus_tag="ACHKW0_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HFO7476993.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(2508..3482)
                     /gene="ghrB"
                     /locus_tag="ACHKW0_004268"
     CDS             complement(2508..3482)
                     /gene="ghrB"
                     /locus_tag="ACHKW0_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HFO7476994.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3586..4245)
                     /gene="yiaD"
                     /locus_tag="ACHKW0_004269"
     CDS             complement(3586..4245)
                     /gene="yiaD"
                     /locus_tag="ACHKW0_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HFO7476995.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            4398..6731
                     /gene="bisC"
                     /locus_tag="ACHKW0_004270"
     CDS             4398..6731
                     /gene="bisC"
                     /locus_tag="ACHKW0_004270"
                     /EC_number="1.8.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HFO7476996.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSHGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6700..7140)
                     /gene="yiaC"
                     /locus_tag="ACHKW0_004271"
     CDS             complement(6700..7140)
                     /gene="yiaC"
                     /locus_tag="ACHKW0_004271"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HFO7476997.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7137..7700)
                     /gene="tag"
                     /locus_tag="ACHKW0_004272"
     CDS             complement(7137..7700)
                     /gene="tag"
                     /locus_tag="ACHKW0_004272"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HFO7476998.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7858..8556
                     /gene="yhjY"
                     /locus_tag="ACHKW0_004273"
     CDS             7858..8556
                     /gene="yhjY"
                     /locus_tag="ACHKW0_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HFO7476999.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8785..9987
                     /gene="yhjX"
                     /locus_tag="ACHKW0_004274"
     CDS             8785..9987
                     /gene="yhjX"
                     /locus_tag="ACHKW0_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7477000.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSICGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KVLREAHI"
BASE COUNT         2436 a         2448 c         2594 g         2645 t
ORIGIN      
        1 atcaaggaga agaaacaaac ttattaagct agaatagcca cgggtgcttg agactgtttg
       61 tctcaggtat tcaccgaaag gcagacagag aaaagcccca cctgactata aatcaaagtg
      121 aggctaccct atgcctgaac accataaggt tagcctctta ctcgttggaa atcaacacag
      181 ggggctggga atgccgcaga aatatagatt actttcttta atagtgattt gtttcacgct
      241 tttatttttc acctggatga taagagattc actgtgtgaa ttgcatatta aacaggggag
      301 ttatgagctg gcggcatttt tagcctgcaa tttaaaagag taagagtctt cggcgggaag
      361 ttattcccgc ctttcttacg gcgttgcgca ttctcattgc acccaaattt atttttcaca
      421 aaaataatac tagattttat tacgcgatcg attatttatt tcctgaaaaa caaataaaaa
      481 aatccccgcc aaatggcagg gatcttagat tctgtgcttt taagcagaga ttacaggctg
      541 gttacgttac cagctgccgg gcctttagcg ccgctttcga tggtgaagga cactttctga
      601 ccttcgtcca gagatttgta accatcgttc tggatagcag agaagtgtac gaacacgtct
      661 ttagagccat cgtcaggagt gatgaagccg aagcctttgt cagcgttgaa ccattttacg
      721 ataccagtca ttttaccgga catagtgtat tacctttaat aattaagtgt gcctttcggc
      781 gatatggcgt gctttacaga ttttgaagcg ttaaaggaat gtgcactacg aggggtatca
      841 acgataactc ttgaagggac ttgccttact acactgcttt aatggtctgt acgtcaaacc
      901 gttgatgtac attaagccac gcattggcgg gtgatgcaac aattattttt catatttatg
      961 attaatcggt tggaaaacgg tcctgtcatc aggaccgtaa acagcaataa agtggataaa
     1021 agtctattcc atcaactgct tacttaatgc cgggttggct tgaatcaaac gcatcaattt
     1081 tagttcggca cttgaaggct tcacgcgtct ggattcccat tcctttacca tggcgactga
     1141 tacgcccaaa acccgggcga aatcatcgat ttttaaccct gtcccttttc gtaactgctc
     1201 aatttcggta caggacgttg ttctgtgcgt cagggtaatt ttctgcgttt catctttaaa
     1261 aacaatctgt tccaggctgc tcaacagctc atgcattgga tctttatatt ccattgaaaa
     1321 ctcctcaaat cacactgcgg gatcgtgaat tgcatcgaag ctcattaaga atagtcggga
     1381 aaagcagaca cagcggggtg aagtcagggc atgctctggc tatgattcga ttcagcctgt
     1441 catttagcgt gacttatagc cacctaacga tctgataaat gccgatatat ctcttaccac
     1501 tcctgttttg taaagatttc gaaaatctaa ccttctgcga aggcgtaaat ctctggctac
     1561 aaaaaaggca acggcactat cattttttaa caatgaaaca acaaattaac cttgtttcat
     1621 tttctccatg attttaccag gaagattacg tagaaagggt atcctgcaag cctgtcctgg
     1681 actatccttg tcacgtcaga cacgcgtgtg tcgttgtgcg ctttttttgg gtgaaaggag
     1741 taagaaaatg gcgacaggaa agtcctgctc tcgctggttt gcgcctcttg cggcgttatt
     1801 aatggtagtt agcctgagtg ggtgttttga taaagaaggc gatcagcgta aagcgtttat
     1861 cgacttcctg cagaatacag tgatgcgtag cggtgaacgt ctaccaaccc tgactgccga
     1921 tcagaaaaaa cagtttggtc cttttgtctc tgattacgcg attctgtatg gttattctca
     1981 gcaggtgaat caggcgatgg attccggtct gcgcccggtc gtagacagcg ttaacgcaat
     2041 tcgcgtaccg caggattacg ttacgcaaag cggtccgctg cgtgaaatga acggttcgct
     2101 gggtgtgctg gcgcaacaac tgcaaaatgc gaagttgcag gccgatgctg cgcactctgc
     2161 gctaaaacag accgatgatt tgaaaccggt ctttgatcag gcattcacca aagtggtaac
     2221 gacgccagct gatgcattgc aaccgttgat tccggctgca caaactttca cgcaacaact
     2281 ggtgatggtt ggagactaca ttgctcagca aggtactcag gtgagctttg tcgcaaatgg
     2341 catccagttc ccgacttcac agcaggcaag tgaatataac aaactgattg cgccattacc
     2401 ggcacaacat caggcgttta atcaggcctg gactacagca gttactgcaa cccaataaag
     2461 agtaaaagcc cgagcgatta tcgggcttta cttcacgcag tcgcggctta gtccgcgacg
     2521 tgcggattca cacagttctt ctcaaccttt ccttgtaacg catcaatcaa attatccacg
     2581 gcacaggcgg ccatgccata acgcgtctca tgggtggcag atccaatatg cggtactgcg
     2641 acgacgttgg ccattgagag caacggcgaa tctacggaca gtggctcttg ttcgaagaca
     2701 tccagcccgg cggcgtgaat ttcgcctttc tgcaatgcag caatcagtgc attttcatca
     2761 accaccgggc cacgtccggc attaatgaaa atggcggagg atttcatttt ggcgaattgt
     2821 tctgcgccaa acagatgatg cgtctcatca gttaacggca ggatcaggca aacgaaatct
     2881 gactcttgta acagagtatc caaatcgcag tagcgggcgt tgaagcgttc ttctgcttct
     2941 ttatggtggc ggcgcgcgtt atagaggatg ggcatgttga agccaaagtg cgcacgttgt
     3001 gccagcgcca taccgatccg tcccatcccg acaatgccca gtgttttatg gtgaacgtca
     3061 gtgccgtacc agtccgggcc tatgctcgcg gtccattcgc ctgcttttac ccgttctgct
     3121 acctccacaa cccgacgagc ggtagacaac accagcgcca tcagcgtatc ggcgacggtt
     3181 tctgttaata cggttggcgt gtgcatcagc agaatttttc gggcggtaag cgcatcgaca
     3241 tcaaaattgt catagccgac ggagatcgtt gatgtggcac gcagtttcgg cattttttcc
     3301 agcaatgcgg catcaacatt ctcgtttgaa cccagtaaac cttcagcttc ggcaaaaatt
     3361 gctgcatttt gttcgacggt ttgtgggctg aggtttgcca cctggtgaac ggtgaaatgc
     3421 tcttgcaggc gttgcagtaa atcatcaggt aaggctttgt agaggataac ggacggcttc
     3481 atgcttctct ccattcactg ataaggcgat caccttagcc tgaaatatga tgaaggaaaa
     3541 ctgaagaatg ccaggtgatt tctgcatcac ctggcatgaa agggattaca gcgggcttaa
     3601 ggtaatttct acacggcggt tttgcgcctt accttctgcg gtgctgttgc tggcgattgg
     3661 gttagccggg ccaaggccct gagtacggat gcggctggcg tccacgccct gggtgatcaa
     3721 cgcgctggca acggaatccg cacgttgctg ggagagacgc atgttcaggt cgtgaccacc
     3781 cgtgctgtcg gtataaccaa tcacgttaac cgccgttttc ggatactctt tcagcaccat
     3841 tgccacgccg gtcagggtgt tagcgcccgc aggtttcagg gtcgcgctgc tgctgtcgaa
     3901 ggtcacattg ttcggcatat tgaggataat gttatccccg ctgcgggtta cgctaacacc
     3961 agtgccgcgc attttgtcac gcagcttcgc ttcctgcaca tccatgtaat aacccacgcc
     4021 gccgcccaga gctgcgcctg ctgctgcgcc aatcagcgcg cctttaccgc gatctttctt
     4081 cgaagaagag agcgcaccaa tacccgcgcc cacgagagag cccagacctg cgccgatagc
     4141 agatttacct gcttcgcgtt cgccggtgta agggttagtt gtgcagccag ataccgccag
     4201 agcgccactc actacggcgg caataagata aacacgtttc ttcattgtta atccttaata
     4261 acctttttat tctttgccac gggttccgtg gcgggagatt atgccgcgtg aacatgaaga
     4321 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
     4381 tttcaggagt tttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
     4441 attgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg catggcgcgt
     4501 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
     4561 cacgggtacg atttccaatg gtgcgcaaag gctttcttgc gtcaccggaa aatccgcaag
     4621 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
     4681 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
     4741 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
     4801 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggcgcg gcacaggcga
     4861 tcatgccgta tgtcgtgggt ggtagcgaag tttatcaaca gcaaaccagt tggccgctgg
     4921 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
     4981 ttgcgtggaa tgcatctgat gagcaggggc tttcttactt ttccgcactg cgtgacagcg
     5041 ggaaaaagct gatctgtatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
     5101 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
     5161 atacgctggt ggaaaatggt tggcacgacg aagcgtttct gacgcgttgc accacaggtt
     5221 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa aacgccgaat
     5281 gggcggcaga gatttgtggg gttaacgcag cgaaaatccg cgagctggcg gctcttttcc
     5341 accaaaacac gaccatgctg atggctggct ggggaatgca gcgccaacag tttggtgagc
     5401 aaaaacactg gatgatcgtc acgctggcgg caatgttggg gcaaatcggc acacccggcg
     5461 gcggttttgg tctttcttat cattttgcca atggtggtaa ccccacgcgg cgttctgcgg
     5521 tgctctcttc catgcagggc agcttgccgg gtggctgcga tgcggtggat aaaatccctg
     5581 ttgcccgcat tgttgaagca ctggaaaacc ctggtggcgc atatcaacac aacggtatga
     5641 accgacattt cccggatatt cgttttatct ggtgggcggg cggtgccaac tttactcatc
     5701 atcaggatac caatcgtctg atccgtgcct ggcaaaaacc ggagctggtg gtgatctctg
     5761 aatgcttctg gacggcggcg gcgaaacacg cggatatcgt tctgcctgcg actacctctt
     5821 ttgagcgtaa tgatctcacc atgaccggtg attacagtaa tcagcatctg gtgccgatga
     5881 agcaagtggt gccgccacgc tatgaagcgc gtaatgattt tgatgttttt gccgagttga
     5941 gtgaacgttg ggagaagggc ggctatgcgc ggtttacgga aggaaaaagt cagctgcaat
     6001 ggctggaaac gttttataac gttgcccggc agcgcggggc aagccagcag gttgaattgc
     6061 cgccatttgc tgagttctgg caagccaacc agttaattga gatgccggaa aacccggaca
     6121 gcgagcggtt tattcgcttc gctgattttt gccgcgatcc gctggcgcat ccgttaaaaa
     6181 ccgccagcgg caagattgaa atcttctcac agcgtattgc cgattacggt tacccggatt
     6241 gccctgggca tccaatgtgg ctggagccgg acgaatggca gggcaatgcc gaaccagaac
     6301 agttgcaggt actttctgcc catccggcgc accgcctgca cagccagctg aattacagtt
     6361 ctctgcgcga attgtacgcg gtagcaaacc gtgagcctgt caccattcat cctgacgatg
     6421 ctcaggcgcg cggcataacc gaaggggata tggtgcgggt gtggaactcc cgtgggcaga
     6481 tccttgccgg agcggtcatt agcgagggaa ttaaacctgg cgtgatttgc attcacgaag
     6541 gggcatggcc ggatctggat ttaaccgctg acggtatttg taaaaacggc gcagtgaacg
     6601 tgctgaccaa agatctcccc agctcgcggc tggggaatgg ctgtgcgggt aatacggcgc
     6661 tggcatggct ggaaaaatac aacggtccgg aactgacact tacagcgttt gaaccaccgg
     6721 ccagctcata atccaggtgg gtagttgggt ttcatcctgc catgcgcaat cgacaatgtg
     6781 aaaaccctgc gcacggtaaa aatctatcgc cggttgattt ttttgataaa cctccagcat
     6841 cagatgggga tagcgctgct gcacatactg catcagcgcc ttaccaatac cgcgtctgac
     6901 ggccttcggt gcgacaaaca ttgctgccag aaatcggcct tccataatgc tgacaaaacc
     6961 gagaagctta ccgtcctctt cccagaccca gttttgcgcg ttggcaagat aggcatcccg
     7021 caccagcgga atgcagtcac gccagtaatt cgcttttata aagggatgcc cccaggttgt
     7081 actttccagc cacagttcga ggatcgcggg gagttctgaa cgttgcgctt cccgaatcat
     7141 ggtttatttc ccggatagca acagcagcca accacatgat cattcaccag cccacatgcc
     7201 tgcataaagg agtaacagat tgtggtgccg acaaacttaa aaccacgttt tttcagtgcc
     7261 ttagataggg cgtcggaggc ggacgtagat gtgggaattt cgctcaacgt tgtggcttgt
     7321 gtcacctgtg gctgatgatt tacaaacgac cagacaaagt cggcaaacgg ttcgccgttc
     7381 tgttccattt gcaggtacgc ccgcgcatta ccaataattg cctgaatttt ccctcgatgg
     7441 cggataatcc cggcgtcctg taccagtctt tcgacatcct cttcctgcat tgctgcgacc
     7501 ttcaccggat cgaactgatg aaagcaggcg cgatagtttt cgcgtttttt gaggacggtg
     7561 atccacgata acccagcctg ctgcccttca aggcagatca tttcgaacag ttttttactg
     7621 tcagtttcag gcacgcccca ttcattatca tggtaggcaa tataaagcgg gtcctgactc
     7681 acccagccgc aacgttccat actttccctc gctatgtacc cgatcgtaaa aatttcagcc
     7741 gcttttcttg acgcatcggt gaaaaagtca atatttaata cagggctact atgcccgata
     7801 tccttcatgc aatgacaata atattgccga attcggctct tccctggagt cgtgttaatg
     7861 atcataaaaa aaagcggtgg tcgctggcag ctaagcctgc tggcgagcgt ggtaatcagt
     7921 gccttttttc tcaacacagc ttacgcctgg caacaagaat atatcgttga tacgcaaccc
     7981 ggacattcca cagagcgtta cacctgggat agtgatcatc aacctgatta caacgatatt
     8041 ttgtcgcaac gtattcaaag tagccaaagg gcgctgggac tggaagtcaa tctggcggaa
     8101 gaaactcctg tggatgtgac cagcagtatg agtatgggct ggaattttcc tttatatgaa
     8161 caggttacaa ccggcccggt cgcggcatta cattacgatg gcacaaccac ctcgatgtat
     8221 aacgagtttg gcgacagtac taccacgctg accgatccgt tatggcatgc cagcgtgagt
     8281 agcttaggct ggcgtgttga ctcccggctt ggcgatctcc gaccctgggc gcaaatcagc
     8341 tataaccagc aatttggcga gaatatctgg aaggcgcaat caggcctgag ccggatgacg
     8401 gcgacaaacc agaacggcaa ctggctggat gtcaccgtag gcgctgatat gttgctcaat
     8461 caaaatattg ccgcctatgc cgcgctaact caggcagaaa ataccactaa taatagcgac
     8521 tatctgtata cgatgggggt tagcgccaga ttttaacgta acagtcacaa ttgaaaccat
     8581 tgaataacaa cagttgtggc gatagtgggt gctaacttac caaataataa atttggtgaa
     8641 taattgtcgc gtcattcatt cctgaactaa ggcatttcat tccgttctga tggcatttca
     8701 tgccgttttt ccccaggcat aaaatgcact tcgttatggt tgtcggcaga gatttttcct
     8761 ttttattact gcaggaatac tgccatgaca ccttcaaatt atcagcgtac ccgctggctg
     8821 acactcatcg gtactatcat tacccagttt gcactggggt cggtttatac ctggagcctg
     8881 tttaatggcg cgctttctgc caagctggat gcgccggtaa gccaggtcgc tttctctttc
     8941 ggcttgttaa gtctggggct ggcaatttcg tcttctgttg cgggcaaatt gcaggaacgt
     9001 tttggcgtta aacgcgtcac catggcttcc ggcattttgc tgggattagg cttcttcctg
     9061 accgcgcatt ccaacaacct gatgatgctg tggttaagcg ccggtgtgct ggtcggtcta
     9121 gcggatggcg cgggttacct gctgacgctc tctaactgcg tgaagtggtt cccggagcgt
     9181 aaaggtctga tatccgcgtt cgctatcggt tcttatggtc tgggtagcct gggtttcaaa
     9241 tttatcgaca cgcagctgct ggaaacggtg ggtctggaaa aaacctttgt gatttgggga
     9301 gcgattgcgc tggtgatgat tgttttcggc gcaacgttaa tgaaagacgc accaaaacag
     9361 gaagtgaaaa ccagcaatgg cgtagtggag aaagattaca cgctggcaga gtcgatgcgt
     9421 aaaccgcagt actggatgtt agcggtaatg ttcctgactg cctgcatgag cggcctgtac
     9481 gtgattgggg tagcgaaaga tatcgcccaa agtctggcac accttgatgt ggtttccgca
     9541 gccaatgcag taacggttat ttccatcgct aacctttcag gtcgtctggt gctgggcatt
     9601 ctgtctgaca aaattgcccg tatccgtgtt atcaccattg gtcaggtgat ttcgctggtg
     9661 ggtatggcgg ccctgctgtt tgcaccatta aatgcagtga cgttctttgc agcgattgcc
     9721 tgcgtggcat ttaactttgg cggcactatt accgtctttc catcactggt cagtgagttc
     9781 tttggcctca ataacctggc gaaaaactac ggtgtgattt acctcggttt cggtatcggt
     9841 agcatttgtg gttcgattat cgcctcactg tttggcggct tctatgtgac cttctacgtg
     9901 attttcgccc tgctgattct gtcattggcg ctttctacga caattcgtca gccagagcag
     9961 aaagtattgc gtgaggcgca tatttagaaa tatgttgcta ctgaaataaa tgcgaggagg
    10021 gagacttcaa agtctccctt tttttatgga cgcatattaa aagaacaata atatttacac
    10081 cacaaatata ttttatttgt ttatatatca tgtaaatata aat
//
LOCUS       DAZFXT010000158        10027 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.158, whole genome shotgun sequence.
ACCESSION   DAZFXT010000158 DAZFXT010000000
VERSION     DAZFXT010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10027)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10027)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10027
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.158"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..149)
                     /locus_tag="ACHKW0_004275"
                     /pseudo
     CDS             complement(1..149)
                     /locus_tag="ACHKW0_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    complement(<1..84)
                     /locus_tag="ACHKW0_004275"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            complement(535..1728)
                     /locus_tag="ACHKW0_004276"
     CDS             complement(535..1728)
                     /locus_tag="ACHKW0_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7477001.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPERKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRAIK"
     gene            1864..3588
                     /gene="iucA"
                     /locus_tag="ACHKW0_004277"
     CDS             1864..3588
                     /gene="iucA"
                     /locus_tag="ACHKW0_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HFO7477002.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELIIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHAHTQQAIDARHDWTALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3589..4536
                     /gene="iucB"
                     /locus_tag="ACHKW0_004278"
     CDS             3589..4536
                     /gene="iucB"
                     /locus_tag="ACHKW0_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase IucB"
                     /protein_id="HFO7477003.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWCEEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4536..6278
                     /gene="iucC"
                     /locus_tag="ACHKW0_004279"
     CDS             4536..6278
                     /gene="iucC"
                     /locus_tag="ACHKW0_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIS family aerobactin synthetase IucC"
                     /protein_id="HFO7477004.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDQDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6275..7612
                     /gene="iucD"
                     /locus_tag="ACHKW0_004280"
     CDS             6275..7612
                     /gene="iucD"
                     /locus_tag="ACHKW0_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase
                     IucD"
                     /protein_id="HFO7477005.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDRGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAAALTHYTTNIQE"
     gene            7618..9813
                     /gene="iutA"
                     /locus_tag="ACHKW0_004281"
     CDS             7618..9813
                     /gene="iutA"
                     /locus_tag="ACHKW0_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HFO7477006.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLRQELVGQVYYRDESLRFYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYISWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWLLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         2369 a         2620 c         2659 g         2379 t
ORIGIN      
        1 gaccccgtac tgctcacgca gcttatccag cagtggcatc attttttcca gaggcggtcg
       61 aactccgcct tcgcaaaata agcggaagcc tggcgaagga tatcgttact gcggcgcagt
      121 tcacgatttt cacgctccag ctctttcagg tgtcaacgcc actctgagag agttttatgc
      181 atcccgtatt gggcactatg atgccggaaa tctgcgaggc tcgacacctc tctgattttc
      241 ccctttattt atcacggtgc cccagatcta atgttgggga tgcgttcatc tcattcccaa
      301 tgagtaatcg cactgttctc attgtctgca ggcatccgga cagagccatg tgatgttgca
      361 ctcacaacca gcatgacttc aatgcaactt aagtgacacc ggccggtttt ataccgaaca
      421 taccctgtac catgcggtgc atttttgcca gtgttactgc ctgacatgct ttcatcagac
      481 gcaggttctc tctcatatcc tctgagttac tccggtaagg tgatgatcat atctttattt
      541 gatagcacgg taatgccaga taaatacaga cacaatggta aataatatag ttgcaaataa
      601 tgccgccata atcagcgcga ttgcgacacc tgttttcgat accagcggca ccagcaaagc
      661 gcacagtcca tagccaagtg tgtgactggt agcgagccag cccgcgcctg cgccatcagc
      721 cagcttatca ttcagtagaa gttgataagc gggtgttgcc agagctgctc caaatgacag
      781 cactgcacag cccgggtaaa acaaccatag ctgttcggag agcatgatag ccagaccacc
      841 actcatcaac actccggcac tcaggagcag ggccaccgga gtcaggcgct gcggacggag
      901 taccccgaac tgcgcgataa gcgcagctac tgcggacaga cccaacagcc aggccacctg
      961 ttggctaatg gcggtggtat ccgtggcgaa ctggcgagta agggcaggcg aaagtccaag
     1021 ctgcatcatg ctcaccgccg cagccagtaa tattgcgcaa agcagataag gcagacaatc
     1081 ccgcttcaga ctgacgctct tgcgctccgg tgtgggctgt ggtggtgtgc cgggcaaccg
     1141 cagcagcatc agcagcgcca gcactggggc tgccatcagc agccccagtg gcgccagagg
     1201 atgaatggcc agcattgctg ccgcgcacag cggcccgaat agcctgccgc aactcaggcc
     1261 ggagctgatg gttgccagag cggccatgcg attcccttct cctgctctct gcaatgccca
     1321 gacctgacag gctggcacca ttgccgacac ggtcagcccg taggcgatcc gggcgatgat
     1381 taatattccc agtcccaccg ctgttgttat ggcgctggta gccatcagca cgcttcccag
     1441 ccctagcagg gtaaagctta tgccatatcc tcccagagcc cacaacacca caggtttact
     1501 gccagtacgg gaaatttgct ttccccacca cggggacgat ggcagaaaca gcattgagcc
     1561 aagcatcagc agggcagccc atacagacag actcagattt gtctgtatga ccagaacagg
     1621 gagcacaacc agcagaccgt tctgcccgat accgagaagc ccggcactga acgcaagtgg
     1681 ccagcaggac agtggttttt ggggcgtatc ttcgatccca ggtgacattt taatgtttca
     1741 actccatgta ttaattgtgt ttatttgtaa aattaattta tctgacaata acatttctta
     1801 ttgataatga gaatcattat tgacataatt gttattattt tactgtgtgg gagctgtttg
     1861 actatgaccc tgccctctga aaaaccagcc acagatgtgg ctgcgcagtg cttcctgaat
     1921 gcactgattc gtgaaactac agactggaaa ctgacagaat acccgccaga cgaattgatc
     1981 atcccgctgg atgagcagaa atcgctccat ttcagagtgg cttatttctc cccaacccag
     2041 catcaccgct ttgcatttcc tgcacgtctg gtcacggcat caggcagtta tcctgtcgac
     2101 tttaccactc tctcccggct tattattgat aagctacggc atcaactgtt tctgcccgtt
     2161 cccctctgcg aaactttcca ccagcgcgtg ctggaaagcc acgcccatac gcaacaggca
     2221 attgatgccc gtcatgactg gaccgccctg cgtgaaaaag cgttgaattt tggcgaagct
     2281 gagcaggcgc tgctgacagg acacgctttc caccctgcgc ctaagtctca tgaaccgttt
     2341 aaccggcggg aggctgaacg ctacctgcct gatatggcac cccacttccc actgcggtgg
     2401 ttttcggtgg ataaaacgca aatcgccggt gaaagtttgc atctcaacct tcaacagcgg
     2461 ctgacgcgat ttgccgcaga gaatgcgcct cagttactca acgaattaag tgacaaccaa
     2521 tggctgttcc cgttgcaccc gtggcagggg gaatatcttt tgcagcaggg gtggtgccag
     2581 gcacttgttg ctaaggggct gattaaagac ttaggtgagg ccggcacgtc gtggctgccg
     2641 accacctctt cccgttccct ctactgtgcc accagccgcg atatgatcaa gttctccctg
     2701 agcgtacggc tgaccaactc catccgtact ctgtccgtga aagaagtgaa gcgtgggatg
     2761 cgcctggcac gcctggctca aaccgacggc tggcagatgc tacaggtccg cttcccgact
     2821 ttccgggtaa tgcaggagga tggctgggcc gggctgctcg atcttaacgg caacatcatg
     2881 caggaaagtc tgtttgccct gcgtgaaaat ctgctggtgg atcagccgaa aagccagacc
     2941 aacgtactgg tctccctgac tcaggccgca cctgatggcg gtgattcgct gctggtttcg
     3001 gcggtaaaac gcctgagcga tcgcctcggt atcactgtgc aacaggccgc ccatgcatgg
     3061 gtcgatgcat actgtcagca agtgctaaag ccgctgttta cggctgaagc ggattacggc
     3121 ctggtgctgc tggcgcatca gcaaaatatt cttgtccaga tgcttgggga tctgccggtc
     3181 ggatttattt accgtgactg tcagggcagc gcttttatgc ctcacgcgac agactggctc
     3241 gattctattg gcgaggcgca ggcggaaaat attttcaccc atgagcagtt gctgcgctat
     3301 ttcccttatt acctgctggt taactccact tttgctgtga ccgctgcgct gggggctgcc
     3361 gggctggaca gcgaatcgaa tctgatggct cgtgtacgag catcgctggc tgaagtgcgt
     3421 gatcaggtga ctcataaaac ctgtctcaac tacgtgctgg aaagtccgta ctggaacgta
     3481 aaaggtaact ttttctgtta tctgaacgat cataacgaga acaccattgt tgacccttcg
     3541 gtgatctatt tcgatttcgc taacccgctg caggctcagg aggtctgaat gtctgaggca
     3601 aacattattc acagcagata tggactgcgc tgtgaaaaac tcgacaagcc cctgaatctt
     3661 ggctggggac tggacaatag cgcggtgttg cactgtcccg gggagctgcc gacagggtgg
     3721 ctgtgcgatg cgctggatca gatatttatc gccgcaccac aactgtcagc agtggctctc
     3781 ccctgggccg aatggtgtga ggagccacag gcgctgacgc tttttgggca ggtaaaaagc
     3841 gacattatcc atcgtaccgc cttctggcag ttaccgttat ggttgagttc accggcaaac
     3901 cgggcctccg gtgaaatggt ttttgatgca gagcgtgaga tttatttccc gcagcgccca
     3961 ccccgtccgc agggtgaggt ttaccgccgt tacgatccac gtattcgcag gatgctgagt
     4021 ttccgcattg ccgatcccgt ttctgatgca gaacgtttca cccgctggat gaatgatccg
     4081 cgcgttgagt atttctggga gcaaagtggc tcactggagg tacagaccgc ctatctggag
     4141 cgccagctca ccggtaaaca tgcattcccg ctgattgggt gcttcgacga tcggccattc
     4201 agctattttg aaatctactg ggcggcggaa gaccgcattg gtcgtcacta ttcgtggcag
     4261 ccctttgacc gtggcctgca tctgctggtt ggtgaacagc aatggcgcgg ggcccactat
     4321 gtgcaaagct ggctgcgcgg gctgacacat tacctgctgc tggatgagcc ccgtacgcag
     4381 cgcacagtac tggagccacg caccgataac cagcgcctgt tccgccatct tgagcctgcg
     4441 ggataccgga caattaaaga gttcgacttc ccacataagc gctcgcgcat ggtgatggcg
     4501 gatcgtcatc acttcttcac ggaggtcggt ctgtaatgaa tcacaaggac tgggattttg
     4561 ttaaccgtcg gctggtggca aaaatgttgt ctgagatgga gtatgagcag gttttccacg
     4621 cggaatctca gggcgatgac cactactgca ttaacctgcc gggagcacaa tggcgcttta
     4681 tcgctgaacg tggtatctgg ggctggctgt ggattgatgc ccaaacgttg cgctgcacgg
     4741 acgaaccagt actggcgcag acgctgctga tgcagctaaa gccggtgctg tcaatgagtg
     4801 atgccactgt tgcagagcat atgcaggatt tgtatgccac gctactgggc gacctgcaac
     4861 tgctgaaggc ccgtcgcgga ctgagtgcca gtgacctgat taatcttgat gccgatcgtc
     4921 tgcaatgcct gctgagcggt catcctaaat tcgtttttaa taaaggtcgc cgtggctggg
     4981 gcaaagaggc gctggaacga tatgcgccag agtatgccaa caccttcaga ctgcactggc
     5041 tggcggtaaa acgtgaacat atgatctggc gctgcgacaa cgatctggat attcagcagt
     5101 tgttgactgc cgcaatggat ccgcaggagt ttacacgctt cagtcaggtc tggcaggaaa
     5161 acgggctgga tcacaactgg ctgccgctgc cggtacatcc gtggcagtgg cagcaaaaaa
     5221 tcgccaccga cttcatcgct gattttgccg aaggcaggat ggtgtctctc ggcgagtttg
     5281 gcgaccagtg gctcgcccag cagtcgctgc gtaccctgac caacgccagc cgccggggag
     5341 ggctggatat caagctgccg ctgaccatct acaacacctc atgctaccgg gggatccctg
     5401 gcagatatat cgctgccgga ccactggctt cacgctggct gcaacaggtt tttgcgaccg
     5461 acgccaccct ggtgcaaagc ggcgcagtga tccttggtga accggctgca ggctatgtgt
     5521 cccatgaagg ctatgccgcg cttgcccggg ctccctatcg ctaccaggaa atgcttggtg
     5581 ttatctggcg ggagaatccg tgccgctggc tgaaaccgga tgaaagtccg gttctgatgg
     5641 caacactgat ggagtgcgac gaaaacaatc agccgctggc aggcgcatat atcgaccgct
     5701 ccggactgga cgctgaaacc tggcttacgc aattgttccg ggtggttgtg gttccgctgt
     5761 atcacctgct ttgccgctac ggtgtcgcgc ttattgcaca cggacaaaat ataacgctcg
     5821 ccatgaaaga gggggtacca cagcgtgttc tgctgaaaga cttccagggc gatatgcggc
     5881 tggtgaaaga agcgtttccc gaaatggact ctttgcctca ggaggtgcgt gatgtcacat
     5941 cccgtctgag tgcggactac ttaatccatg atttgcagac gggtcacttc gtgacagtac
     6001 tgcgttttat ttcgccactg atggttcgtc ttggcgtacc tgaaaggcga ttttatcaac
     6061 tgctggcagc agtgttgagt gattacatga acaaacatcc acaaatgtca gagcgttttg
     6121 cgcttttctc actcttcagg ccacaaatca ttcgcgtggt gctgaacccg gtaaaactga
     6181 cctggccgga ccaggatggc ggcagtcgca tgctgccgaa ttaccttgag aatctgcaaa
     6241 atccgctgtg gctggtaact caggaatatg aatcatgaaa aaaagtgtcg attttattgg
     6301 tgtcgggaca gggccattta atctcagtat tgctgcattg tcacatcaga tcgaagaact
     6361 gaactgtctc ttctttgacg aacatcctca tttttcctgg catccgggta tgctggtacc
     6421 ggattgtcat atgcagactg tctttctgaa agatctggtc agtgcagttg cacctacaaa
     6481 tccctacagc tttgttaact atctggtgaa gcacaaaaag ttctatcgct tccttacaag
     6541 cagactacgt acagtatccc gtgaagagtt ttctgactat ctccgctggg ctgctgaaga
     6601 tatgaataac ctgtatttca gtcataccgt tgaaaatatt gatttcgaca aaaaaagtcg
     6661 attgtttctg gttcagacca gtcggggaga atattttgcc cgcaatatct gccttggtac
     6721 aggaaaacaa ccttatttac caccctgtgt gaagcatgtg acacaatcct gtttccatgc
     6781 cagtgaaatg aatcttcgtc ggccggacct gagtggaaaa cggataactg tggttggtgg
     6841 aggacagagt ggtgcagacc tgttccttaa tgcattacgc ggggaatggg gagaagcggc
     6901 ggaaataaac tgggtctcac ggcgtaataa ttttaacgca ctggatgagg ctgcttttgc
     6961 tgatgagtat tttacacctg aatatatttc aggcttctcc ggactgaagg aagatattcg
     7021 ccatcagtta ctggatgagc agaaaatgac atcggatggc atcactgccg attctttact
     7081 gaccatttat cgtgagttgt accaccgttt tgaagttctg agaaaaccaa gaaatatccg
     7141 tctgctaccc agccgctcgg taacaactct ggaaagtagt ggtccgggct ggaagttatt
     7201 gatggaacat catctggatc ggggcagaga gagcctggaa agtgatgtgg tgattttcgc
     7261 cacaggttac cgttctgcat tgccacaaat acttccctca ctgatgccct tgatcaccat
     7321 gcacgataag aacaccttta aagtgcgtga tgacttcact ctggaatgga gtggcccgaa
     7381 agaaaataac atctttgcgg tcaacgccag catgcaaact catggcatcg ccgaacccca
     7441 gctcagcctg atggcctgga gatctgcacg tattcttaat cgcgtactgg ggcgtgattt
     7501 attcgatctc agtatgccgc ccgcactgat tcagtggcgc agcggcagcc ggaaaaaacc
     7561 gcagccggag gctgctgctt taactcacta tacaacaaat attcaggaat aacgatgatg
     7621 cgcaaaaagt atatgccccg ggctcttggt ccgctgcttc ttgtcgtgct gtcaccagct
     7681 gtcgcccagc aaaacgatga taatgagatc atagtgtctg ccagccgcag caatcgaact
     7741 gtagcggaga tggcgcaaac cacctgggtt atcgaaaatg ccgaactgga gcagcagatt
     7801 cagggcggta aagagctgaa agacgcactg gctcagttaa tccccggcct tgatgtcagc
     7861 agccagagcc gaaccaacta cggtatgaac atgcgtggcc gcccgctggt tgtcctgatt
     7921 gacggtgtgc gcctcaactc ttcacgttcc gacagccgac aactggactc tgtcgatcct
     7981 tttaatatcg accatattga agtgatctcc ggcgcgacgg ccctgtacgg tggcgggagt
     8041 accggagggt tgatcaacat cgtgaccaaa aaaggccagc cggaaaccat gatggagttt
     8101 gaggctggca caaaaagtgg ctttaacagc agtaaagatc acgatgagcg cattgccggt
     8161 gctgtctccg gcggaaatga ccatatctcc ggacgtcttt ccgtggcata tcagaaattt
     8221 ggcggctggt ttgacggtaa cggcgatgcc accctgcttg ataacaccca gaccggcctg
     8281 cagcactcca atcggctgga catcatggga accggtacgc tgaacatcga tgaatcccgg
     8341 cagcttcaac tgataacgca gtactataaa agtcaggggg acgacaatta cgggcttaat
     8401 ctcgggaaag gcttttccgc catcagcggg agcagcacac catacgtcag taaggggctg
     8461 aattctgacc gcattcccgg cactgagcgg catttgatca gcctgcagta ctctgacagt
     8521 gatttcctga gacaggaact ggtcggtcag gtttactacc gcgatgagtc gttgcggttc
     8581 tacccgttcc cgacggtaaa tgcgaataaa caggcgacgg ctttctcctc gtcacagcag
     8641 gataccgacc agtacggcat gaaactgact ctgaacagcc aacttatgga cggctggcaa
     8701 atcacctggg ggctggatgc tgagcatgag cgctttacct ccaaccagat gttcttcgat
     8761 ctggctcagg caagtgcttc cggagggctg aacaaccata agatttacac caccgggcgc
     8821 tatccgtcat atgacatcac caatctggcg gccttcctgc aatccagcta tgacattaat
     8881 gatattttta ccgtcagcgg tggcgtacgc tatcagtata ctgagaacag ggtagatgat
     8941 tttatcgact acacgcagca acagaagatt gctgccggga aggcgatatc tgccgacgcc
     9001 attcctggtg gttcggtaga ttacgataac tttctgttca atgctggtct gctgatgcac
     9061 atcaccgaac gtcagcaggc atggttcaat ttttcccagg gggtggcatt gccggatccg
     9121 gggaaatatt atggtcgcgg catctatggt gcagcagtga acggccatct tcccctgaca
     9181 aagagcgtga acgtcagcga cagtaagctg gaaggcgtga aagtcgattc ttatgaactg
     9241 ggctggcgct ttaccggtga caacctgcgg actcaaatcg cagcatatta ctcgctttcc
     9301 aataagagcg tggaaaggaa taaagatctg accatcagtg tgaaggacga caggcgccgt
     9361 atttacggcg tggaaggtgc ggtggactac ctgatcccgg atactgactg gagtaccggt
     9421 gtgaacttca atgtgctgaa aaccgagtcg aaagtgaacg gtcaatggca aaaatatgac
     9481 gtgaaggaat caagtccatc gaaagcgaca gcttacatta gctgggcgcc ggaaccgtgg
     9541 agtctgcgtg tgcagagcac cacttctttc gacgtaagcg atgcagaggg taacgatatt
     9601 aatggttaca ctaccgtcga ttttatcagt agttggctgc ttccggtggg aacactcagc
     9661 ttcagcgttg agaacctctt cgaccgtgac tataccactg tctggggaca gcgtgcacct
     9721 ctgtactaca gcccgggtta cggccctgct tcactgtacg actacaaagg ccggggccga
     9781 acctttggtc tgaactactc agtgctgttc tgacggggat tcttttgcaa caagggtatg
     9841 ctgtaaacaa catggtcctt ggtggccatg ttgtttacag ccagtataat atacagcctt
     9901 aacaagatat tccgtctccg gaatggactg ttcaccactg aataacaata tgtctgtttt
     9961 atggatcttt gactgaaatt tatcgcatat cctcatttca ttgtccgtct gaaaactaac
    10021 cttacaa
//
LOCUS       DAZFXT010000159         9930 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.159, whole genome shotgun sequence.
ACCESSION   DAZFXT010000159 DAZFXT010000000
VERSION     DAZFXT010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.159"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(4..1124)
                     /locus_tag="ACHKW0_004282"
                     /pseudo
     CDS             complement(4..1124)
                     /locus_tag="ACHKW0_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260381.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4113 domain-containing protein"
     gene            1047..1464
                     /locus_tag="ACHKW0_004283"
                     /pseudo
     CDS             1047..1464
                     /locus_tag="ACHKW0_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1468..1773
                     /locus_tag="ACHKW0_004284"
     CDS             1468..1773
                     /locus_tag="ACHKW0_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477007.1"
                     /translation="MTETGGQPPVSFPVKDVAGLLFLLRRLTRRGRNAACGQCPASGR
                     ARDGRFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSSVGLTRYRTR"
     gene            1850..2533
                     /locus_tag="ACHKW0_004285"
     CDS             1850..2533
                     /locus_tag="ACHKW0_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO7477008.1"
                     /translation="MSRFIQGDCVRVMATFPGNAVDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKTYT
                     SKAAYVGYRHECAYILAKGRPALPQKPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            2534..2755
                     /locus_tag="ACHKW0_004286"
     CDS             2534..2755
                     /locus_tag="ACHKW0_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477009.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            2715..3201
                     /locus_tag="ACHKW0_004287"
                     /pseudo
     CDS             2715..3201
                     /locus_tag="ACHKW0_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052646.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            3247..4023
                     /locus_tag="ACHKW0_004288"
     CDS             3247..4023
                     /locus_tag="ACHKW0_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477010.1"
                     /translation="MNENTTLNALICRHARDLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIHKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDTLRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCLEHILSSGLACDV
                     LRIPDEPPRQWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            4440..4841
                     /locus_tag="ACHKW0_004289"
     CDS             4440..4841
                     /locus_tag="ACHKW0_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7477011.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLGFLYAEASVYVLAKQMMP
                     EYEGGFWHFIRLPDGDRFHLVNGANWFDRTVSADAAGIILTSLVINRQLWLYHDSGDA
                     GLTHLYRMRDAQSWSHIEVHPECNAIYAALD"
     gene            4888..5310
                     /locus_tag="ACHKW0_004290"
     CDS             4888..5310
                     /locus_tag="ACHKW0_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7477012.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIARDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            5307..5498
                     /locus_tag="ACHKW0_004291"
     CDS             5307..5498
                     /locus_tag="ACHKW0_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477013.1"
                     /translation="MNINTETREILRNYRAVINVRRRDMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            6044..6250
                     /locus_tag="ACHKW0_004292"
     CDS             6044..6250
                     /locus_tag="ACHKW0_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000547968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7477014.1"
                     /translation="MYIPQNFLPKSRQKLLRSAPGQSFPAVAVMPLAAKVLKKVMVNT
                     GGNKRQIPAKRQNRGSRNSKDDGG"
     gene            6275..6561
                     /locus_tag="ACHKW0_004293"
                     /pseudo
     CDS             6275..6561
                     /locus_tag="ACHKW0_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001825190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6680..7497
                     /locus_tag="ACHKW0_004294"
                     /pseudo
     CDS             6680..7497
                     /locus_tag="ACHKW0_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            7776..9113
                     /locus_tag="ACHKW0_004295"
     CDS             7776..9113
                     /locus_tag="ACHKW0_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO7477015.1"
                     /translation="MPLLNDLLDFSDHPLMPPPSAQLFAGHLPVEWIQHCLTLSAHAT
                     VRRRRLPGDMVIWMVVAMAFFRNEPITDVVRRLNLSADGEAGMNLLARSAVTQARQRV
                     GAAPVEWLFRQTAQTWGSERYLKDDWHGLQLFAIDGAPFRTPDEPELREYYGSANTST
                     ERQSAYPVMRLVALMNLGSHILLNAVTAPYRRSETVPAHSMLATIPDNSITLFDKLFY
                     SADLLLTLNQQGCNRHWLLPAWKNIAAETEESYGPGDRLLKLKVSLQARKKNPALPEF
                     WYARAVTYEVNGVEKTVQTSLPADRYKAKEVAELYHSRWEIEVGFRNLKSSLLNNALV
                     LRSRKVELLEQEVWGMLLAYNLIRREATKAAEKHKKAASEISFKFAFQFIATEMIVLG
                     NTASPGTIPKRLEHLRGALEVVFITKRPRPSRPRAVKISKTRYPVKRSTAPLK"
     gene            complement(9234..9836)
                     /locus_tag="ACHKW0_004296"
     CDS             complement(9234..9836)
                     /locus_tag="ACHKW0_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HFO7477016.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NEICHATDCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTEHQNQ
                     RRLAYASDVYRIYTGIKSSKGIQIPATKKSLPEINSVQNN"
BASE COUNT         2217 a         2600 c         2784 g         2329 t
ORIGIN      
        1 ttttcaaagt ctggcgaccg gtagttcgtt ccagcgggta gtgtatgccg gtgacgacat
       61 ctcacgcttt atctgccatt ctggcgcaat gcctcggcct gcaaaccaca ctttccccag
      121 accggaatgg ttaatgctgt ccacgacttt caacggctgg ttactgttgc tccggggctg
      181 agtatcatcg aaaaggttca aatgcgaaac cccggtagga gtgaaatcgt tgagtatgca
      241 acccgctttt gcatatcgat ggccatccac ccatatcctg tccagaattc taacggcagc
      301 ggcaatgata tcccgtgtat cctgcgtggg gatgagcagt ttttcactgg ccacattccc
      361 gtaatacggt tcgtttactg cgaacggtga cgtcttcaaa aaaatggcga tatgtctgca
      421 gaactgacac tcgccccgca gcttttcagc tgcacgctca gcgtaccgac agatggccta
      481 gcgcatggcc tcatatgtcg tgacgcgctc tctaaaggac cggctgcaga ctatctgctg
      541 tttggggggg cggttcttcc tccagtgaaa tacagctttc tccattgagt tcccggacct
      601 ttctctccag aaccacattg aagttcttcc tgataaatac agggttcgtg cgccagctgc
      661 agtgacagca gtttttccgc acggcggata ttgtccggcg ttaatgccag cacgccacca
      721 aactgtggcc actctttgga agcccattgt gttgactttg ccaacgtttt ggttggcccc
      781 attccgacac cgatagtcag gccagtcccg ttcctgacgt gttcacgcag ctgctggtcg
      841 aaatcctcga aatcgatgca gctgtcaata cctcgcacgt cgagaaacat ctcgtcaatc
      901 gagtactgct caacgcgcgg tgccagcgcc tccagatgaa ccatgaccct gttcgacatc
      961 gatgcgtata gcgggtaatt actggagaag acgatgaccg gctccgaaaa tttcaccgac
     1021 ttcagctgaa accacggtac cccattttga tgcccagccg cctggatgtt gaagtaaacg
     1081 gctttaacgg tggtgttctg aacggtgttc cttctgcata tcactggtat accgaacagt
     1141 atggcgtgaa gtggcctgtg gggtatgagg tgaatatcag cagtcagaga gacaacttca
     1201 ttcaggtgga ttttgacacg ccgtggtgtc agccagaaag cgacgttatt gcagaattaa
     1261 gccgccgttt cagttgcaca ctggagcact ggtatgccga acagggctgt gatttctgtg
     1321 gctggcagtt gtatgagcgt ggagagctcg ttgatgtgct gtggggggga acttgaatgg
     1381 tcttccccga cagatgacga tgagctgccg gaagtcaccg gaccggcgtg gatagtcgac
     1441 aatgtggcgc attatggagg atgaagtatg acggagacgg gcgggcaacc gcccgtttct
     1501 tttccggtaa aggatgtcgc cgggctcctc tttttactgc gccggctgac gcggcgcggc
     1561 aggaacgctg cctgtggtca gtgtcccgcg tccggccggg cacgggacgg gcgcttttac
     1621 cgctcccggc tggcgttggt gacagtgtac gccagcccgt cgcccttttc tgatgaacgc
     1681 ccttcaagcc gctttcgcgg catatcctcg ccgtcaaaaa gacgacggct gcggtattcc
     1741 tcggtcggcc tgacccgtta ccggacgcgg tgaacagccc acaggcagcg gggaacgggc
     1801 gccacagggg cgccctcccg gtgccctctg taagagaagg agtgctgtta tgtcccgttt
     1861 tatccagggt gactgcgtgc gtgttatggc cacgtttccg ggcaatgctg tcgatttcat
     1921 cctcaccgac ccgccatatc tcgtcggttt ccgtgaccgt caggggcgta ccatcgccgg
     1981 cgataaaacc gatgaatggc tgcaaccggc ctgtaatgaa atgtaccgag tgctgaaaaa
     2041 agatgcgctg atggtgagct tctatggctg gaaccgcgtc gatcgcttta tggccgcctg
     2101 gaaaaatgcg ggattcagcg tggttggcca cctggtattt accaaaacct acacatcgaa
     2161 ggccgcatat gtgggctatc gccacgaatg cgcttacatc ctggcaaaag ggcgtccggc
     2221 gctgcctcag aagccattgc ccgacgtgct gggctggaaa tattcaggca accgccatca
     2281 cccgacggaa aagcccgtta ccagcctgca accactgatt gagagcttca cacatccgaa
     2341 cgcaattgtg ctggacccgt ttgcaggcag cggctcaacc tgcgtggccg cccttcagtc
     2401 cggacgccgg tatatcggta tcgagctgct tgaacagtat caccgtgccg gacagcaacg
     2461 actggccgcc gtgcaacggg ccatgcagca gggggccgcg aatgataact ggtttgaacc
     2521 ggaggctgcg taaatgaact atgccggaca cgaaaaactg cgcgccgaag tggcggaggt
     2581 ggccaatgcc atgtgcgacc tgcgtacaac catgaatgag atggagcagc ggtacagctt
     2641 taatgccgac accctgccgg aacgtctggt gcgtcagacg ctgtttcgcg caaaccgcct
     2701 cttgatggag gcatataccg aaattctgga actggattcc tgcttcaaag attgagggga
     2761 gagagtatgt acggaacatg cgaaacgctc tgccgggagt tggcagcgca gtatccggga
     2821 gacactccgc tgatgctggt tgtctggtcc ccggaagaga ttcaggtcct cgctgatgga
     2881 atggatattt ccctgtccga tcatgaaatc aggaccgtcc tggcgcggct ggaggacatc
     2941 ccggaagacc agcggactga gtccggtatt tcttccggtg cggctatgga gattatcagc
     3001 aacgtgagtg aaaaccgcca ggtgaccgtc cctgctgaac tgctggcgtc cctgattcag
     3061 accgctgaac aggcattgtg gaagcgtgaa tgggccgccc gtgataacgg cctcgccgtc
     3121 ccggaattcg tcacccgccg tcaggcggtg gttaatcagg cccgcatcct gctgaaaaac
     3181 aacacgcacg aaaacgactg atgttatcgc cgcctccggg cggcgataca gggagaacga
     3241 ttaatcatga acgaaaacac aacgctgaat gcactgattt gtcgtcacgc ccgcgacctg
     3301 ctgctggcgc agggctggcc ggaagagact gatgttgacc agcggaaccc gaagtatccg
     3361 ggctggatca gcatttatgt tctgctggat gcgccccggc tggcgacgtt acttatcaac
     3421 cgtcacggcg gcgtactgcc gccgctccta gcctccgcta ttcacaaact gaccggaacc
     3481 ggggcggaac ttgtactgtc cggcagtcag tggcagtcgc tgccggtact tccggcagac
     3541 ggaacgcagg tgtcttttcc gtatgccggt gaatggctga ccgaagacga aatcagggct
     3601 gtacttgcgg cggtgcgtga tgctgtacgc agtgtcagtt gccgggtggc agaagatacg
     3661 ctgcgtatcc gggcggcgct gaccaccacc ggtcagacgt tactgacccg ccagacgcgc
     3721 cgctttcgcc tagtcgtgaa ggaaagcgat cacccctgct ggctcgatga ggacgacgaa
     3781 aacctgcccg tggtgctcga tgccatcctg aaccggggcg cacgtttttc ggcggtggaa
     3841 atgtatctgg tcagcgattg tcttgaacat atcctgtcca gtgggctggc ctgcgatgtg
     3901 ctgcgtatac cggatgaacc gccccgccag tggtttgacc gtggtgttct gcgggaagtt
     3961 gtccgggaag cccgggccga aatccgcagc atggcggatg ccctggcaaa aatccggaaa
     4021 tgatgacgtt ggcggggtaa atccccgcct ttttcctgcc ggtccccgga cagagctcaa
     4081 aggaagtcgc caccggctcc gctttacccg gccatgcggg gcatggcctt gtgggttttc
     4141 agttatgtgg ccgccgtgtt gtgtgcgggc tgtgccgtgc ctccatctta gccgggctgg
     4201 cggggatgca agggtacgct tcgccgctgc ggtcacccgg tccctccttc ccgtctgccg
     4261 tgattttccg gtcgttcacc cgctcaggtt tcgcggtctc accctccaac tcccgccagc
     4321 cgtgtgcgtc aggctgcggc ttcccttgcg cccctgcatc cccgccttat cgcccggctt
     4381 ttatggaggc acggcaccgc ccggtgtcgc ggaatatgtg aacaacggag gaaaacatca
     4441 tgcaatatgc gaaacctgtc actctgaatg ttgaagagtg cgaccgtctc tcctttctgc
     4501 cttacctgtt tggcctgggt tttctgtatg ccgaagcgtc tgtgtacgtg ctggcgaaac
     4561 aaatgatgcc ggaatatgaa ggcggcttct ggcacttcat ccgcctgccg gacggtgacc
     4621 gttttcatct ggttaacggt gcaaactggt ttgaccgcac ggtcagcgcc gatgccgcag
     4681 gcatcattct gacttccctt gtgattaacc gccagttgtg gctgtaccac gacagcggtg
     4741 atgcgggact gacacacctg taccggatgc gtgatgcgca gtcgtggagc cacatcgaag
     4801 ttcatcccga atgtaacgcg atttacgcgg cactggactg attaacggac ggggcggcaa
     4861 tgccgcccct gtaaaggaga cgagaacatg tactgtactg ttaaagaaat tatccgcgaa
     4921 gtgctggata cagacgtgcc ggacagtgaa tgcgtttttg ccgtggtgct gacccgtggg
     4981 gatgtgcgtc acatagcccg ggactggagt ctgacagacg atgagctgga aaccgtcatg
     5041 caacggctgg acgatgcttt tgagcatggt gcggatgtca gcattgttca cgacgttgtc
     5101 cgtgaactga tggaagaaaa gcgtgccagc cgtcaggtga cagtcccggc agtgatgctt
     5161 gaaaaagtga tggcgctggc aggcagtgaa atgaagcgcc tgtatgccgt cgggagtgag
     5221 aacgggggcg acggtgacgc attcgtcagg gaagaacgcg aagcaatgga cgttgtgtta
     5281 caggcgctgg acggggagca catgtcatga atatcaacac ggaaacccgc gaaattctgc
     5341 gtaattacag ggccgtgatt aatgtgcggc gtcgtgatat ggggcagaaa ccgctcacca
     5401 ccgcgcagat tgttgatgaa atctgcgatt ttgtggcgaa tcagcaggcg gttttcctcg
     5461 gtggtcacta tatccttcag ggcagcagaa acaggtgata cagggcaggc ggtggaggcc
     5521 gcctgtcagc ccgcgcgaaa acccctggca ggctgacgcc tgccggaagg gaagcgggcc
     5581 atgctgacgc gtcgcccgtt acccttcctg tttgtgatgt gtccggaatg tttttttctg
     5641 atgttatttt tctatggtta ccgtgcttgt tattgttctt cttctttttc ctcttcctct
     5701 tctttccgtt cctctcctgt ttatggcttt tccgggcgtc cccctggcgg ggcgggctgt
     5761 cgtgaacgga gccagcgaag ctgtctccgc cctggcgggc ttccatccct gacgcgaaac
     5821 cctggcttgc agcgaccgtt ccggtcgcac cgcctctgtc gcgccgtggc ctgcgctcct
     5881 ggcattccgg caaaaggccg ccgggatgta aggggcaacg ccgtttcgcg cacgcgctct
     5941 ccgtcgttgc ggcgggtccc tgcccgttgt tgtgtccggt gccattcacc tgtttaagcc
     6001 cctgtcgccg ggggcaaaga cgtctccgcc agtccagggt gaaatgtaca tcccgcaaaa
     6061 ctttttgccc aaaagcaggc aaaaactttt gcggtctgcc cccggacagt cgtttccggc
     6121 cgtggcagtg atgccccttg cggcgaaggt tctcaaaaag gtgatggtga ataccggagg
     6181 gaacaaaagg cagatccccg caaaacgtca aaaccgcggc tcccgaaact caaaagatga
     6241 tgggggctga aaaccagaga gaggagaaag caggatgagt acccgaaata tccacgttaa
     6301 caccgcgtcg tacacgcttc ttgttgccgg gaagaaaaaa acaccgggga agagtgggac
     6361 gtcctggaat tcagcagcct gactgagctg aaaaaatatc gcaaaagcca cccggaaaag
     6421 atggccttca gctatagcta cgcgctcagc cggggtgtgg atacgcagtt ccgccatatc
     6481 aacattgcgg aagccgatca cttcaaacag ttcctgcgtc agattaagcg tgccggcctc
     6541 gatatccggg cgatctgctg accagatgtg atgaatattt ctcctgattg ttgcaatctt
     6601 gttaatagtg accgggaggg atgatattaa gcggaaaagt tttttattaa atttattctt
     6661 tcataaagga gtagctgtta tgcgattagc ttcccgtttt ggtcggtata attccatccg
     6721 ccgtgaacgt cctttaacgg atgatgaatt aatgcagttc gtgccttcgg tattttccgg
     6781 tgataaacat gagtcccgga gtgaacgtta tacgtatatt ccaacaatat catcaataag
     6841 ttacgtgatg aaggtttcca gccattcttt gcctgtcaga gtcgggttcg tgatttggga
     6901 cgtcgcgaat acagtaaaca tatgttacgt cttcgcaggg aagggcatat taacgggcag
     6961 gaagttcctg aaattatcct gcttaattca catgatggtt catccagtta tcagatgatc
     7021 cccggaattt ttcgttttgt ctgcacaaat ggcctggtgt gcggaaataa ttttggcgaa
     7081 atccgcgttc cacataaagg tgatattgtc gggcaggtta tcgagggagc gtatgaagta
     7141 ctcggtgtct ttgataaggt cactgataat atggaggcga tgaaagaaat tcatcttaac
     7201 agtgacgagc aacatttatt tggcagagct gcactgatgg tcaggtatga agatgaaaat
     7261 aaaacgccag tgacgcctga acagataatt actccccgtc gtcgggagaa taaacagaac
     7321 gatctctgga caacctggca gcgggctcag gagaatatga taaaaggtgg attatcgggg
     7381 cgaagtgcct ccgggaaaaa taccaggaca agagccatta caggtattga tggtgatata
     7441 agaatcaaca aggcgttatg ggtgattgcc gaacagttca gaaagtggaa gtcatgataa
     7501 tataagtaaa gccccgaaaa tttttcgggg ctttacttat attatcattc ttcggcatcg
     7561 ctgtgtcgtc tgatatacct caatttcatc aactggcttt ccggttgtct gtaaatattg
     7621 tgcccgcgaa aaatggtctg gcaggaggct gcgcagttac cggttatata taagatgggt
     7681 tcccgcttcg cgcaatgccg atcagttaag gatcggttga ccgatcctta aactgcggca
     7741 ctataacggc ttccacaaca gggagccgtt ttcttatgcc acttctcaat gatctgctcg
     7801 atttcagtga ccatccgctt atgccaccgc cctctgcaca actgtttgca gggcaccttc
     7861 ccgtcgagtg gatacagcac tgtctgacgc tttctgcgca tgcgaccgtt cgccgccgtc
     7921 gtttaccggg ggacatggtt atctggatgg tggtggccat ggcctttttc cgcaatgagc
     7981 caattaccga tgtggttcgc cgtctgaacc tgagcgcgga tggcgaagcg gggatgaacc
     8041 tgctggcccg cagcgctgtc acccaggcgc gtcagcgcgt gggggccgcc ccggtggaat
     8101 ggctcttccg ccagaccgca cagacatggg gctcggaacg ttacctgaag gatgactggc
     8161 acggcctgca gctttttgcc attgatggcg caccgttcag gacacctgat gaacctgagt
     8221 tgcgtgaata ttatggctct gccaacacat ccactgagcg gcagagcgcc tacccggtca
     8281 tgcgtctggt ggcgttgatg aatctgggaa gccacatttt gctgaatgcc gtgaccgcgc
     8341 cttaccggcg gagcgaaacc gtgccggctc actccatgct cgccactatc ccggataact
     8401 ccattacgct gtttgacaag ctcttttaca gcgcagacct gctgctgacg ctgaaccagc
     8461 agggttgtaa ccgccactgg ctactgcctg cgtggaagaa tatcgcggca gaaacagaag
     8521 aaagttacgg tcccggagac aggcttctga aactgaaggt gtcgctgcag gcgaggaaaa
     8581 agaatcctgc actgccagag ttctggtacg cccgggcggt gacttatgag gtgaacggtg
     8641 tggagaaaac ggtgcagaca tcactcccgg cagaccgcta caaagcaaaa gaggtagcgg
     8701 aactttacca ttcacgatgg gaaatcgaag tcgggttcag aaacctgaaa agcagcctgc
     8761 tgaacaatgc cctggtgctg agaagccgta aggttgagct gctggaacag gaggtgtggg
     8821 gcatgttgct ggcttacaac ctgatacggc gtgaggcaac aaaagcggct gagaaacaca
     8881 aaaaagcggc gtcagaaatc agctttaagt tcgcgttcca gtttatcgcg acagaaatga
     8941 tagtgctggg aaatacggca tcaccgggga ccatcccgaa acgactggag catctgcggg
     9001 gtgctctgga agttgtgttc ataacaaaac gcccccgacc atcaaggccg agggcagtta
     9061 agatatcaaa aacccgttat ccggtgaaac gcagcaccgc tccgcttaag tgaacggcat
     9121 tgcccgcttc gcggggcttc ggccagtcag tattatcact catatgatat ttttgtgtgt
     9181 ggccttccat gccgcttttg cggcataacc ggtatctgac aatgtctgta agattaattg
     9241 ttctgcacgc tgttaatttc cggaagtgat ttcttcgtgg ccggaatctg gataccttta
     9301 ctgcttttta tcccggtata aatccggtaa acatctgatg cgtaggcaag acgtctctgg
     9361 ttctggtgtt cggtcttcct gaatccggca ttgtatgcac caacggcctc ccaggagacg
     9421 ccccattttt taaaggctat tgccagataa taagcaccgg tataaatgtt catgcaggga
     9481 tctgttgtca gatgttccgg cttaattcca tagcgggcca gttcgttaaa atgctgggaa
     9541 tctacctgca tcagtccgct gccatatccc gttaccggat taataccgat ggcattaacc
     9601 cggtaacggg attctttcca tgatattgct ctcagtaaat caggatctat tttgtaatcc
     9661 cggcctgcaa gatcaaagca atcagtggca tggcagatct catttataaa catcaggcag
     9721 atggctaaca tccatttttt catttttcca cctctggtga ctttatccgt aaataattta
     9781 acccactcca caaaaaggct caacagattg gtggttctca ccaccaaaag caccacaccc
     9841 cacgcaaaaa caagtttttg ctgattttta tttataaata gagttttatg aaaaattagt
     9901 ttctcttact ctctttatga tatttaaaaa
//
LOCUS       DAZFXT010000160         9868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.160, whole genome shotgun sequence.
ACCESSION   DAZFXT010000160 DAZFXT010000000
VERSION     DAZFXT010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.160"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(209..2782)
                     /gene="clpB"
                     /locus_tag="ACHKW0_004297"
     CDS             complement(209..2782)
                     /gene="clpB"
                     /locus_tag="ACHKW0_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HFO7477017.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2912..3643)
                     /gene="yfiH"
                     /locus_tag="ACHKW0_004298"
     CDS             complement(2912..3643)
                     /gene="yfiH"
                     /locus_tag="ACHKW0_004298"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphorylase YfiH"
                     /protein_id="HFO7477018.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3640..4620)
                     /gene="rluD"
                     /locus_tag="ACHKW0_004299"
     CDS             complement(3640..4620)
                     /gene="rluD"
                     /locus_tag="ACHKW0_004299"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HFO7477019.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4755..5492
                     /gene="bamD"
                     /locus_tag="ACHKW0_004300"
     CDS             4755..5492
                     /gene="bamD"
                     /locus_tag="ACHKW0_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HFO7477020.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5763..6104
                     /gene="raiA"
                     /locus_tag="ACHKW0_004301"
     CDS             5763..6104
                     /gene="raiA"
                     /locus_tag="ACHKW0_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HFO7477021.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6207..6332
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6208..6255
                     /gene="pheL"
                     /locus_tag="ACHKW0_004302"
     CDS             6208..6255
                     /gene="pheL"
                     /locus_tag="ACHKW0_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0031556 - transcriptional attenuation by
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HFO7477022.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6354..7514
                     /gene="pheA"
                     /locus_tag="ACHKW0_004303"
     CDS             6354..7514
                     /gene="pheA"
                     /locus_tag="ACHKW0_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HFO7477023.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLKSAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7557..8678)
                     /gene="tyrA"
                     /locus_tag="ACHKW0_004304"
     CDS             complement(7557..8678)
                     /gene="tyrA"
                     /locus_tag="ACHKW0_004304"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HFO7477024.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8689..9759)
                     /gene="aroF"
                     /locus_tag="ACHKW0_004305"
     CDS             complement(8689..9759)
                     /gene="aroF"
                     /locus_tag="ACHKW0_004305"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HFO7477025.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
BASE COUNT         2506 a         2631 c         2323 g         2408 t
ORIGIN      
        1 aattctcagg agaaccccgc tgacccggcg gcgtgtttgc cgttgttccg tgtcagtggt
       61 ggcgcattat agggagttat tccggcctga caagcgaaaa atataaaaac tttatcgttc
      121 gctcattttt caggcaaaac atcttaaata tagtcttttc cgtctaactt atagacaaaa
      181 acgagccccg aagggctcgt tttatcattt actggacggc gacaatccgg tcttcattaa
      241 cttccaggcg aatcacttta cccggaacca attcaccaga cagtatttgc tgtgccagcg
      301 ggttttcgat ctgctgctgg atagcacgtt tcaaaggacg tgcaccatag actggatcgt
      361 aaccgttctc gctcagcagt ttcagcgcct cgtcagaaat gtggatttca taaccacgtt
      421 cttccagacg tttgtacaga cgtttcaact gaatctgcgc aatcgaggca atgtgctgtt
      481 cacccagcgg atggaagacc accacttcat caatacggtt aatgaattcc ggacggaagt
      541 tatggcttac cacaccgagc accagctctt tcatgtgcgc ataatccagt tcaccgaagc
      601 gttcctgaat caaatcggaa ccgaggttag aggtcataat gacaaccgta ttacggaagt
      661 cgaccgttct cccctgcccg tcagtcagac gaccatcatc cagtacctgc aacagaatgt
      721 tgaagacatc cggatgcgct ttttccactt catccagcag gatgacggaa tacggacgac
      781 gacgcacagc ttcggtcagg tagccacctt cttcataacc gacatatccc ggaggcgcac
      841 caaccagacg agacaccgag tgtttctcca taaactcgga catatcgata cggaccatcg
      901 cctcgtcgct atcaaacata aagttcgcca gcgccttaca aagctcagtt ttccccacac
      961 ccgttgggcc gaggaacagg aatgaaccaa tcgggcgatt tggatccgcc aaccccgcac
     1021 ggctacggcg aatagcgtta gataccgcat caaccgcttc gttctgacca atgacgcgat
     1081 ggtgcagttc ttgctccata cgcagcagtt tttcgcgctc gctttccatc atgcgagaaa
     1141 ccggaatccc cgtccaacgc gccagcactt cagcaatttc ggcgtcggtc actttattac
     1201 gcaacagacg catagttttg ccttcgagct gcgttgcggc ttccagttgc ttttccagtt
     1261 ccgggatttt gccgtattgc agttcagaca tccgcgccag gtcccccaca cggcgagcct
     1321 gttcaatagc gattttcgcc tgttccagtt ccgctttaat ggtctgcgta ccagaaagcg
     1381 atgccttctc tgctttccac tcttcttcta actcggagta ctgacgttct ttgtcgctca
     1441 gttcttcgtt gagcatatcc aggcgttttt tactggcttc atcagactct ttcattaacg
     1501 cctgttgttc cagtttgagc tggatgatac gacgatcgag tcggtcgagt tcttctggtt
     1561 ttgagtcaat ctgcatacga atgctggatg ctgcttcatc gatcaggtcg atggctttat
     1621 ccggcagctg acggtcagca atgtagcgat gagacaacgt cgccgctgca acaattgccg
     1681 ggtcagtaat ttgcacatgg tggtgcaatt cgtaacgttc tttcaggcca cgcagaatcg
     1741 caatggtatc ttcaacagaa ggctcggcaa caaacacttt ctggaaacga cgttccagcg
     1801 cagcatcttt ttcgatgtac tggcgatatt cgtcaagcgt cgtggcacct acgcagtgca
     1861 attcaccacg cgccagcgcc ggtttcagca tgtttccggc gtccattgcg ccatcggctt
     1921 tacccgcgcc gaccatggta tgtaattcgt cgataaacag gatgacgttg ccttcctgtt
     1981 tggcaagatc gttaagcacg ccttttaaac gttcttcaaa ctcaccgcga tatttcgccc
     2041 cagccaccag cgcgcccata tccagcgcca gtacgcggcg gcctttcaat ccttccggca
     2101 cttcgccgtt gataatacgc tgcgccagac cttcaacgat ggcagtttta ccgacgccgg
     2161 gttcaccaat cagtaccggg ttatttttag tacgacgttg cagcacctga atggtacggc
     2221 gaatttcttc atcacgacca atcaccggat cgagtttgcc ctgttcggct cgttcggtaa
     2281 ggtcgatggt atattttttc aaagcctgac gttggtcttc agcaccttga tcgttcacgc
     2341 tttcacctcc acgcatttgt tcaatcgctt gagtaatgtt ggcggtggtc gcccctgctg
     2401 ctttcaggat gtcggccagc gtgccgcgag actcaagtgc cgccagaacg aacagttctg
     2461 acgagataaa gttatcacca cgtttttgcg ccagcttgtc gcaaagatta agaacgcgca
     2521 ccagatcctg tgatggctgg acatcaccac cagtaccttc aacctgcggt aaacgattta
     2581 atgcctgatt gatatctgtg cgcaactggc cagcatttat gccagcggat gttaataaag
     2641 gactaaccga acccccttcc tgattcagca gggcgctcat taaatgaagt ggttcgataa
     2701 attggttgtc gtgcccgagt gcaagtgatt gggcatcggc aagagcaagc tggaatttat
     2761 tagtaagacg atccagacgc ataactcctc ccataacgga tcaaagttgc tactggagat
     2821 taaatgaggt catccctcaa ttattcaagg ttattgacca gattaatgtg aaaagaaaat
     2881 cacgcgtacc ggatcgtctt gattctttag gttatatcag ccaaatgaaa cttgccatac
     2941 gaccggtggt cttgtcgcga cgataagaga agaaagtctc attttccgta tatgtacaac
     3001 ggtcgccgcc gaaaatttgc tcaacaccca cgttcgccag acgctgccgg gcaagctgat
     3061 aaatatccgc cagatactta tcaccatgct gaatgaaagc tgcacttgct ttagcgtcta
     3121 ctgccataaa cgcctcgcga acctccaccc ccacttcgaa cgcgcgtgga ccaattgccg
     3181 gccctaacca ggcgagaata ttttccggat tatcagcaaa acaggaaacc gtctcttcca
     3241 gcacgcctgc gcacagtcca cgccagccag catgagcggc ggcgacttcc gttcccgctc
     3301 gattgcaaaa cagcacaggg aggcagtcgg cagtcatcac tgcgcaaacc gtgccgggcg
     3361 tattgctata agaggcatct gctcgttttg aggcataagg ttcgccagta agcttaagca
     3421 catctttgcc gtgtacctgc tcaagccaga ccggtttaga aggcaaattg cccgcagcaa
     3481 aaagtcgctt gcgattctcc tcaacgtgat ccgggttatc gccacaatgg gcaccgaggt
     3541 tgagcgagtc atacgggggc aaactcacgc cgccgatacg agaagagcta caggccgcaa
     3601 caccttttgg tagcggccac tgcgggacaa tcagcttact cataaccagt ccacttcatc
     3661 cttatgctct tcgaaatcgg cgcgcatcac ctcaatcagc tccaccatat cttgtggaat
     3721 aggcgcatgc cattccattt cgatgccgga gatcgggtga taaagacgca gcatggttgc
     3781 atgaagcgcc tggcggtcaa acttacgcag catggagata aatgcttccg aagcaccttt
     3841 tggcggacgc ggacggccac cataaaccgg atcgcccacc agcggatgag tgatatgggc
     3901 catatgcacg cggatctggt gcgtacgtcc agtttccaga cgcaaccgca gacgcgtgtg
     3961 cacacggaag tgttccatga tgcgatagtg agtcaccgcc ggtttgccca tcggatgcac
     4021 cgccatgtga gtacgtttgg tcgggtggcg gctgatgggt tcatctactg taccgccagc
     4081 ggtcatatga ccaatcgcca ccgcttcata ctcacgagta atttcacgcc gttgcaaaga
     4141 ttcgactaaa cgcgtctgag ccggaacggt ttttgccaca accatcaggc cagtggtgtc
     4201 tttatccaga cgatggacga tgcccgcacg tggtacatcg gcaatgggcg gatagtaatg
     4261 aagcaacgca ttcagtaccg tgccatccgg gttacccgcg ccaggatgta ccaccaggtc
     4321 gcgcggttta ttaatgacga tgatatcttc atcttcatag acgatatcca gcgggatatc
     4381 ctgcggttca aaacgcgctt cttcttcaat ctcagcgttg atggcaacct gctcgccacc
     4441 caatactttt tctttcggct tatcacaaac tttgccgttt accagcacgc gctggtcgag
     4501 gatccattct tttattcgcg aacgtgaata atccgggaac atttcggcca aagcctgatc
     4561 taagcgttga ccgagttggt tttcggacac cgttgcagtg agctgtactc gttgtgccat
     4621 atatactgct tcttcgttta tcgttggggt tttacggctt tgccgtttaa tatagtgtgc
     4681 tattgtagct ggtcttaacc gggagcagga acagagaatc tcccgtatta cattttgagg
     4741 aaagtcaaaa cgtcatgacg cgcatgaaat atctggtggc agccgccaca ctaagcctgt
     4801 ttttggcggg ttgctcgggg tcaaaggaag aagtacctga taatccgcca aatgaaattt
     4861 acgcgactgc acaacaaaag ctgcaggacg gtaactggag acaggcaata acgcaactgg
     4921 aagcgttaga taatcgctat ccgtttggtc cgtattcgca gcaggtgcag ctggatctca
     4981 tctacgccta ctataaaaac gccgatttgc cgttagcgca ggctgccatc gatcgtttta
     5041 ttcgccttaa cccgacccat ccgaatatcg attatgtcat gtacatgcgt ggcctgacca
     5101 atatggcgct ggatgacagt gcgctgcaag ggttctttgg cgttgaccgt agcgatcgcg
     5161 atcctcaaca tgcacgagct gcgtttagtg acttttccaa actggtgcgc ggctatccaa
     5221 acagtcagta caccaccgat gccaccaaac gtctggtatt cctgaaagat cgtctggcga
     5281 aatatgaata ctccgtggcc gagtactata cagaacgtgg cgcatgggtt gccgtcgtta
     5341 accgcgtaga aggaatgttg cgcgactacc cggataccca ggctacgcgt gatgcgctgc
     5401 cgctgatgga aaatgcatac cgtcagatgc agatgaatgc gcaagctgaa aaagtagcga
     5461 aaatcatcgc cgcaaacagc agcaatacat aacagaaacc tgaaatacaa aacggcagcc
     5521 cttgagctgc cgttttttta ttctgttagt tgtgaaactg aagcgattta gtcactatcg
     5581 atctcatcaa atatggctcg ctttgagata ttcctcaagt aaaaaaacac ctcttcctgc
     5641 gatttctcac aaaaaagatt cgttgacaaa aagtgacaaa attatgagat tcccatcaca
     5701 cattttgaca tcaggagcgg tatgctgaat tcaccaagac gggaagacaa gaggtaaaat
     5761 ttatgacaat gaacattacc agcaaacaaa tggaaattac tccggccatc cgccaacatg
     5821 tcgcagaccg tctcgccaaa ctggaaaaat ggcaaacaca tctgattaat ccacatatca
     5881 ttctgtccaa agagccacaa gggtttgttg ctgacgccac aatcaataca cctaacggcg
     5941 ttctggttgc cagtggtaaa catgaagata tgtacactgc aattaacgaa ttgatcaaca
     6001 agctggaacg gcagctcaat aaactgcagc acaaaggcga agcacgtcgt gccgcaacat
     6061 cggtgaaaga cgccaacttc gtcgaagaag ttgaagaaga gtagtccttt atattgagtg
     6121 tatcgccaac gcgccttcgg gcgcgttttt tgttgacagc gtgaaaacag tacgggtact
     6181 gtactaaagt cacttaagga aacaaacatg aaacacacac cgtttttctt cgcattcttt
     6241 tttaccttcc cctgaatggg aggcgtttcg tcgtgtgaaa cagaatgcga agacgaacaa
     6301 taaggcctcc caaatcgggg ggcctttttt attgataaca aaaaggcaac actatgacat
     6361 cggaaaaccc gttactggcg ctgcgagaga aaatcagcgc gctggatgaa aaattattag
     6421 cattactggc agagcggcgc gaattggccg tcgaggtagg aaaagccaaa ctgctctcgc
     6481 atcgcccggt acgagatatt gatcgtgaac gcgatttact ggaaagatta attacgctcg
     6541 gtaaagcgca ccatctggac gcccattaca ttactcgcct gttccagctc atcattgaag
     6601 attccgtatt aactcagcag gctttgctcc agcaacatct caataaaatt aatccgcact
     6661 cagcacgcat cgcttttctc ggccccaaag gttcttattc ccatcttgcg gcgcgccagt
     6721 atgctgcccg tcactttgag caattcattg aaagtggctg cgccaaattt gccgatattt
     6781 ttaatcaggt ggaaaccggc caggccgact atgccgtcgt accgattgaa aataccagct
     6841 ccggtgccat aaacgacgtt tacgatctgc tgcaacatac cagcttgtcg attgttggcg
     6901 agatgacgtt aactatcgac cattgtttgt tggtctccgg cacgactgat ttatccacca
     6961 ttaatacggt ctacagccat ccgcagccat tccagcaatg cagcaaattc cttaatcgtt
     7021 atcctcactg gaagattgaa tataccgaaa gtacgtctgc ggcaatggaa aaggttgcac
     7081 aggcaaaatc accgcatgtt gctgcattgg gaagcgaagc tggcggcact ttgtacggtt
     7141 tgcaggtact ggagcgtatt gaagcgaatc agcaacaaaa cttcacccga tttgtggtgt
     7201 tggcacgtaa agccattaac gtgtctgacc aggttccggc gaaaacgacg ttgttaatgg
     7261 cgaccgggca acaagccggt gcgctggttg aagcgttgct ggtgctgcgc aaccacaatc
     7321 tgattatgac ccgtctggaa tcacgcccga ttcacggtaa tccatgggaa gagatgtttt
     7381 atctggatat tcaggccaat cttaaatcag cggaaatgca aaaagcattg aaagagttag
     7441 gggaaattac ccgttcaatg aaggtattgg gctgttaccc aagtgagaac gtagtgcctg
     7501 ttgatccaac ctgatgaaaa ggtgccggat gatgtgaatc atccggcact ggattattac
     7561 tggcggttgt cattcgcctg acgcaataac acgcggcttt cactctgaaa acgctgtgcg
     7621 taatcgccga accagtgctc caccttgcgg aaactgtcaa taaacgcctg cttatcgccc
     7681 tgctccagca actcaatcgc ctcgccgaaa cgcttatagt aacgtttgat taacgccaga
     7741 ttacgctctg acgacataat aatgtcggca taaagctgcg gatcctgagc aaacagtcgc
     7801 ccgaccatcg ccagctcaag gcggtaaatc ggcgaagaga gcgccagaag ttgctcaagc
     7861 tgaacatttt cttctgccag atgcagccca taagcaaaag tagcaaagtg gcgcagagcc
     7921 tgaataaacg ccatattctg atcgtgctcg acagcgctaa tacgatgcag ccgagcgccc
     7981 cagacctgaa tttgctccag aaaccattgg tatgcttccg gcttacgtcc atcacaccag
     8041 accacaactt gctttgccag gctaccgctg tccgggccga acatcgggtg taaccccagt
     8101 accgggccat cgtgcgccgc cagcatggcc tgtaatggtc catttttcac tgatgccaga
     8161 tcaaccagaa tacaatcttt cggtaaaggc ggtaatttgc cgataacttg ctcagtaacg
     8221 tggattggca cactaacaat caccattccg gcatcggcaa caatatcagc cgctcgatcc
     8281 cagtcatgtt gctccagaat ccgcacctga taacccgaga gggtcagcat cttctcgaac
     8341 aggcgtccca tctgaccgcc accgccgacg ataaccaccg gacgcagtga cggacaaagt
     8401 gttttaaatc ctttgtcgtt ttcactggag taagattcac gcatcacccg acgcaaaaca
     8461 tcctcaatca gatctggcgg tacacccaga gcttccgcct ctgcacgacg cgaggccaac
     8521 atagatgcct cgcgctccgg aacataaata ggcagtccaa agcggctttt cacctcgccc
     8581 acttcagcaa ccagttccag acgcttagct aataaattca gcagcgcttt atcgacttca
     8641 tcaatttgat cgcgtaatgc ggtcaattca gcaaccataa taaacctctt aagccacgcg
     8701 agccgtcagc agcccgttca gatcctgatg aatttcacgc agcaaggcat cggtcatttc
     8761 ccagctaatg caggcatcgg ttacggatac accgtatttc atttcactgc gcggttgctc
     8821 ggaagactga ttgccctcgt ggatattact ttcgatcatc agaccaataa ttgagcgatt
     8881 gccatctttg atttgagcaa ccacggattc tgccaccgca ggctgacggc gataatcttt
     8941 attggaatta ccgtggctgc aatctaccat cagagacggg cgcagtcccg cctgttccat
     9001 ctctttttca cattgcgcaa catccgcagg gctatagttc ggcgctttac caccgcgcag
     9061 gatgacatgc ccgtccggat tcccctgagt ttgtagcaac gcaacctgcc ctgcctggtt
     9121 aatgccaaca aaacggtgcg gctgggcggc ggcgcgcata gcgttaattg ctgttgccag
     9181 actgccgtcg gtgccgtttt taaaaccaac cggcatggaa agcccggagg ccatttcacg
     9241 gtgagtttgc gattccgttg tacgagcacc aattgctgac cagctaaaca gatcgcccag
     9301 gtattgcggg ctattcggat ctaacgcttc cgtcgccagt ggcagtccca tattcaccag
     9361 ctcaagcagc aatttacgcg cgatctgcag cccggcttct acatcaaaag agccatccat
     9421 atggggatcg ttaattaacc ctttccagcc gacagtggta cggggttttt caaaatagac
     9481 gcgcattacc agatagaggc tatcgctgac ctctgcggca agggctttaa atcgacgagc
     9541 atattccaga gcagtttccg gatcatgaat ggaacaagga ccacatacta ccagcagacg
     9601 aggatcgcgc ccggcgataa tatctgaaat ggttttacgc gagtcagcaa tctgggcttc
     9661 ttgttgcagg ctcaatggaa aagcggcctt cagttgttcc ggagtcatta aaacctgttc
     9721 gtcggtaata tgtacgttat tcagcgcgtc tttttgcatg atggcgatcc tgtttatgct
     9781 cgtttgcgat agttgatcct cagcgaggat gacgtaacga taacacataa agtaaagttt
     9841 tcaatccata tttcgtacat ttttattt
//
LOCUS       DAZFXT010000161         9720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.161, whole genome shotgun sequence.
ACCESSION   DAZFXT010000161 DAZFXT010000000
VERSION     DAZFXT010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.161"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            245..1309
                     /gene="ulaG"
                     /locus_tag="ACHKW0_004306"
     CDS             245..1309
                     /gene="ulaG"
                     /locus_tag="ACHKW0_004306"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035460 - L-ascorbate 6-phosphate
                     lactonase activity [Evidence IEA];
                     GO_process: GO:0019854 - L-ascorbic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HFO7477026.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVASGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            1417..2172
                     /gene="ulaR"
                     /locus_tag="ACHKW0_004307"
     CDS             1417..2172
                     /gene="ulaR"
                     /locus_tag="ACHKW0_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HFO7477027.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(2169..2918)
                     /gene="yjfP"
                     /locus_tag="ACHKW0_004308"
     CDS             complement(2169..2918)
                     /gene="yjfP"
                     /locus_tag="ACHKW0_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7477028.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSSDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            3100..3429
                     /gene="bsmA"
                     /locus_tag="ACHKW0_004309"
     CDS             3100..3429
                     /gene="bsmA"
                     /locus_tag="ACHKW0_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HFO7477029.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTNHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            3578..3853
                     /gene="yjfN"
                     /locus_tag="ACHKW0_004310"
     CDS             3578..3853
                     /gene="yjfN"
                     /locus_tag="ACHKW0_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 family protease activator YjfN"
                     /protein_id="HFO7477030.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3970..5595)
                     /locus_tag="ACHKW0_004311"
     CDS             complement(3970..5595)
                     /locus_tag="ACHKW0_004311"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HFO7477031.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5679..6842)
                     /locus_tag="ACHKW0_004312"
     CDS             complement(5679..6842)
                     /locus_tag="ACHKW0_004312"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HFO7477032.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            complement(6845..7483)
                     /locus_tag="ACHKW0_004313"
     CDS             complement(6845..7483)
                     /locus_tag="ACHKW0_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HFO7477033.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESGDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            complement(7493..7891)
                     /locus_tag="ACHKW0_004314"
     CDS             complement(7493..7891)
                     /locus_tag="ACHKW0_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HFO7477034.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            complement(7909..8568)
                     /locus_tag="ACHKW0_004315"
     CDS             complement(7909..8568)
                     /locus_tag="ACHKW0_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HFO7477035.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            complement(8619..9317)
                     /locus_tag="ACHKW0_004316"
     CDS             complement(8619..9317)
                     /locus_tag="ACHKW0_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HFO7477036.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            complement(9336..>9720)
                     /locus_tag="ACHKW0_004317"
     CDS             complement(9336..>9720)
                     /locus_tag="ACHKW0_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YjfI family protein"
                     /protein_id="HFO7477037.1"
                     /translation="ALATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQM
                     IIETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLE
                     DILLEITSQVDNALDLAEITEEYSH"
BASE COUNT         2529 a         2551 c         2304 g         2336 t
ORIGIN      
        1 cgcgacccgc aaataattcc acatggatag aatgggattc acacaacgaa acaactattc
       61 atttaaattc atttagttaa ttgaattaca cattccatta aatctttcca cactcaaaat
      121 caacaaccac ttccctggca aaaagtcaaa aaatggtcat tgccttcctg aaaaaatagt
      181 gtcaggatta atcaaaacca atcacatatt gattcgaatt gaactatcat gaaggtaaat
      241 ggcgatgagt aaagtgaaaa gtatcacccg agaatcctgg atcctgagca ctttcccgga
      301 gtggggtagc tggctgaatg aagaaattga acaagagcag gtcgcctctg gcacatttgc
      361 gatgtggtgg ctaggctgca ccgggatctg gttgaaatca gaaggtggca ccaacgtttg
      421 cgttgatttc tggtgcggca ctggcaaaca aagtcacggt aacccgttaa tgaaacaggg
      481 tcaccagatg cagcgcatgg ctggcgtgaa aaaactgcag ccaaacctac gcaccacccc
      541 gtttgttctt gatccgtttg cgattcgcca gatcgacgcg gtactggcga ctcacgatca
      601 caacgatcat atcgacgtta acgtcgctgc tgccgtgatg cagaactgtg ctgatgacgt
      661 accattcatt ggaccgaaaa cctgcgttga tttgtggatt ggctggggcg taccgaaaga
      721 gcgttgcatc gtggtcaaac cgggcgatgt tgtaaaagtg aaagacattg aaattcatgc
      781 gcttgacgct ttcgaccgta ctgcactgat caccctgcct gccgatcaaa aagcggctgg
      841 cgtactgccg gacggcatgg acgatcgcgc ggtgaactat ctgttcaaaa cgcctggcgg
      901 ctccctgtat cacagcggcg actcccacta ctctaactat tatgcgaagc acggtaacga
      961 acatcagatc gatgtggcgt taggatcgta tggtgagaac ccgcgcggta ttaccgacaa
     1021 aatgaccagc gccgatatgc tgcgtatggg tgaagccctg aatgcgaaag tggttatccc
     1081 gttccaccac gacatctggt caaacttcca ggccgatccg caagagatcc gcgtgctgtg
     1141 ggagatgaaa aaagatcgtc tgaagtatgg cttcaagccg tttatctggc aggttggcgg
     1201 caaatttacc tggccgctgg ataaagacaa cttcgagtac cactatccgc gcggtttcga
     1261 tgattgcttc actattgaac cggatctgcc gttcaagtca ttcctgtaat ctgtcgttta
     1321 taccggatag cgtggagaag ttcgcgctat ccggcgtagt atttcctggc tatttcaaat
     1381 atcatctaaa aaaatcaaat tttatcggaa tagctcatga ctgaagcaca aagacatcaa
     1441 atcctcctgg aaatgctcgc acaattgggc tttgtgaccg ttgagaaagt cgttgagcgt
     1501 ctgggaattt cgcctgccac tgcgcgacgc gatatcaata aacttgacga aagcggcaaa
     1561 ctgaaaaagg tgcgcaatgg cgcagaagct attacccagc agcgcccgcg ctggacgccg
     1621 atgaatctgc atcaggcgca gaatcacgat gaaaaagtac gtatcgctaa agcagcctcg
     1681 cagctggtta atccgggcga aagcgtagtc atcaactgcg gctccaccgc gtttctgctt
     1741 gggcgggaaa tgtgtggcaa gccagtgcaa atcatcacta attatctacc gctggcaaat
     1801 tacctgatcg atcaagaaca tgacagcgtg atcattatgg gtggacagta caacaaaagt
     1861 cagtccatca ctttaagccc gcagggcagc gaaaacagtc tctatgccgg gcactggatg
     1921 tttaccagcg gaaaagggct gaccgcagaa gggttgtata aaaccgatat gctgacagca
     1981 atggcagagc agaagatgct gagcgtggta gggaaactgg tggtactggt tgatagcagt
     2041 aagattggcg aacgcgcggg aatgcttttt agccgtgccg atcaaatcga tatgctgatc
     2101 accggcaaaa atgctaaccc ggaaatcctg caacaactgg aagcgcaagg ggtcagcatt
     2161 ctgcgtgttt aaagatgctg gcggaaaaat gtcacggcag catctaacgc ctcaggcgta
     2221 atgcggtgac gcacgcctgg ctgccatgaa caggttagca gtttatcccg tcccgtctcg
     2281 cttaaggcct gctgcaaacg tagtgattcg tcggcaggca caacgtcatc gtcgaggcca
     2341 tgccacagca gtagaggtct gtccccaagt tgttccaggt ggtttgtcgc ttcccactct
     2401 gccagcggcg cgataatgtt attgaattca ttctgctgtg ctgccgtttc aggtatcagc
     2461 ggtggaaaca gtgaacgggc gagcgatgta aaatagcccg atcccatcat gctggcggta
     2521 catttcaccg tggggtggcg agcggtaatt cccagtgccg tcatcgcgcc catcgacgcg
     2581 ccaccgactg ccagacggtc atcaagcagc cagttttctt cggctattgc cgcacgtaaa
     2641 gtagtgaatt cctgcatact ttgtagcaag atttgccaga attgatttaa ccgccgcgct
     2701 tcgtcgctac taaaacggct accgtgatcg ggcgcatccg gcatgatcac ccgcaaacca
     2761 gcctgcgcca gcgcaacggc aaaatagcta tacaccaggc tggatgaagt aaagccgtga
     2821 taaaaaatta cgcacggtaa cggggtatct ttttgcccga caggataagc atgaagaacg
     2881 ggaatatctg ccagctcgcg tgattctatt tcaatcatct gtctctcctt tttgtctggt
     2941 atgacatgaa gaaatcctta gcacaagttt ctgctggcgg tattagttca taatgttgag
     3001 atgtgggtta cgctttcgtc acgttttcat tcgaaaatcg cgtaccaggt aacaattcgg
     3061 gaacattccc ccaaagttaa attaataagg cactacacta tggttagcag gaaacgtaat
     3121 agcgttattt accggtttgc cagtttatta ttggtgttga tgttaagtgc ctgtagcgca
     3181 ctgcaaggta cgccacagcc agcaccgcca gtaacgaacc atccgcaaga gattcgccgc
     3241 gaccagacgc aagggttaca gcgaataggt agcgtaagca cgatggttcg gggttctccg
     3301 gatgacgcat tagcagaaat cagagcaaaa gctgtcgctg caaaagctga ttattacgtt
     3361 gtcgtcatgg tggacgaaac catcgtgaca ggacagtggt attcacaagc cattttgtac
     3421 cgtaaataaa tcagacaact tacatagtca gctttacatt gctttgcgtc cctatgcgtt
     3481 tacctgtgca caataaatta ccggctggaa gcctgggcgt ttattaccgc gatggaatgc
     3541 cctgcaacat gtggggaaac gaaaaatgga gctgacgatg aaacaattac ttgcctcacc
     3601 ctcgctgcaa ttagtgactt atcctgcgag cgccacggca cagtctgcag aattcgccag
     3661 tgctgactgc gtgacgggcc tgaatgaaat aggtcagatc tcagttagca atatttcagg
     3721 ggatccgcag gacgtggaac gtatcgtggc gttaaaggcc gatgaacaag gtgcgtcatg
     3781 gtaccgcatt attaccatgt acgaagacca gcagcctgat aactggcgcg tacaggcgat
     3841 actgtatgca tgagtgtcat ctacagcaaa tgaacaatgc cggatgcggc gcgagcgcct
     3901 tttccggcct acaattttca catatttcat tgagttaatc gaaaccagcg tcgcatcagt
     3961 cgtatacgct tacacacaca ctccccccgt cgcccgcagc aataaatcat tctggatctg
     4021 ctctgacaac cgtacgccgc cgcgcgtatc taacatcacc tgacaccacg cctgtgccat
     4081 tggcggagag gcatatttca acatttgcgc accgcagccc agcaggaata gctgatgagt
     4141 aatctctcgc cccaattctt cagctggctt gcgcagctgc tgctgtaaac gacgaaccgc
     4201 gcgatcaaaa tagcgatcct gccctttcac ttccacaaac gcttccgaca ataagtcgta
     4261 tacgcccgct tgcttattga gaacgcgcag cacatccagg cacataatat tgccggaacc
     4321 ttcccaaata ctgtttaccg gcatctcccg gtaaagccgc ggcagctcgc tctcctcgca
     4381 ataaccaatg ccgcccagca cctccatcgc ttcggccaca aacggcatac ctcttttgca
     4441 gatcacaaat tttgccgcag gcgtaaataa acgcgcccac agggcttctt tggcatcggc
     4501 acgccggtcc cacgctcgcg caagacgaaa cagcaacgcc gtttgccctt caagctgaag
     4561 tgccatgcga cttaagacat gacgcataag gggctgttgg atcaatggat taccaaaaac
     4621 atggcgttga tgtgcatgat aaatcgccag cgaaaatgca cggcgcatca tggcatggct
     4681 acccagggcg caatcaaaac gcgtcatccc acccattttc aggatcagac gaattccttc
     4741 cccttcctgc cccaacaacc agccaatggc atcctgaaac tccacttcgc agctggcgtt
     4801 agagcgatta cccagcttat ctttcagccg ctcgaggcga atcgcgttgc gttgcccgtc
     4861 aggcaaaaag cgcggcacaa aaaagcagga caaaccgccc gtagtctgcg ccagcaccag
     4921 atgcgcatcg ctttgcggca ccgagaaaaa ccatttatgc cccaccagcc gataagagcc
     4981 atcttccagg cgctctgcgc gggtggtgtt gctcatgaca tcggaaccgc cctgcttttc
     5041 cgtcattccc atgccaatca acaaaccgcg tttttgccca cctggcaata agtgagaatc
     5101 gtagcgatcg ctcagcagcg gcgtggtcca gtcctgaaac ggcgcgggta acatctgtaa
     5161 cagcaatggc gtggcggcaa aggtcatggt tatcggacat aacgaccctg cctcaacctg
     5221 tgcgtgcaac ataaaacgcg ccgcgcgcgc cacaaatgcg ccggagcgag cgtcttcttc
     5281 ccaggcaaga ttgtgcaccc gattggtaca tagcgcctgc atcagcaggt gccaggcggg
     5341 gtgaaaacgc acatcgtcca gacggcgtcc ttgcgcatcg tagcgcaata attcaggcgg
     5401 attcacattc gccagccgcc ccagttcaag ggattcagcc gttcctaact gctgaccaat
     5461 actggctaga aaatcgctat cccagccagc accttcacgc gttaccgctt cgcagagcgc
     5521 gccatcagac aggtataagt tgctgttatt taatggtata ggttgattaa aaacggtgtg
     5581 agtttgccag tgcactgtgt ctccctccat caatggcagt caccattagt atggtcactg
     5641 ccatggattc atgacagaaa gagattgcta aaacattctt atccagcaat gtaatgcgga
     5701 acgaaacgtg aggtgtcttt ggtgatcagt gtgttatctt cacggatccc cattccgcac
     5761 gcttcatcat cgacaatcca gctaccgatg agtgtgtagc tatcgccaaa tcgcggcaga
     5821 ggttgaaacg cctggtagat catcggttca tcggcgtaat caccatcagc gtggtcaacg
     5881 acgttattct gaccgtcgaa aatggtgacg tttccgcctt cgcgcgagta gatcggttta
     5941 cgcacatagc tttcgccagc ggcaatctgc gatttttcac cctcaaacca ggatgcaaga
     6001 aggttaggat gaccagggaa gaagcgccaa agcagcggca ttagcccttt attactcaag
     6061 atacttttcc ataacggctc cacccattgc tcgcgacgct tgcacagcag cggaccgtta
     6121 tcgtcacgca tcatccactc cagcggatac agcttaaatg cacgctggat gacattatca
     6181 tcaagatcgg tcagtacacc gccgacgccc aaaccgagat cttcaatgta gataaaccgc
     6241 gactcctgcc ctgcctgctg ggcgcagtcc tgcaagtaca gcacggtagt cctgtcttca
     6301 tcggtgtcct gacagcagca gaaataaaac ggctcccggc tataaagctc gctaaagcgt
     6361 gaaatcaggc gttcctgaat ggcattgtac tgatcggcat cacgcggaat aatgccgctg
     6421 cgccgggcat cctccagcca caaccactgg aaataagccg actcatacaa cgaggtcggc
     6481 gtatcggcgt tgtactccag cagcttcacc ggcgcattgc cacaccaggc aaaatccatc
     6541 cggccataca gcgaaggatc gcgtgcgcgc cagctttcgg cgatcacatc ccagtacaac
     6601 ggcgggatcg ccagttgcgt caggatctct tcgtctttaa ccgcgcgatc caccacctca
     6661 aggcacatct gatgcagttc cgcagtcggt ttttcgatct gctcttcaat ctggcgcaga
     6721 gtaaaacggt aagcccgact ctcgtcccaa tagatttcat tgtcgatgat atgaaagtcg
     6781 aaaccgttgt tagcggcgat ctggtccaga tcccgtcgca caggaacgtt gtgtctcagc
     6841 atgattagcc tccccaatgc ccacgggcgc tggaagaacg accatagccg ccgcgcgaaa
     6901 cggttgacgc ttttttggtg gtaaagccgc ctgaagagta agactctttt ttgccagaac
     6961 cggagcgcca ggagtaatca ccggaagtgt tgcgccagac ggggcgggaa gcaaatgagg
     7021 atccgccgct gttatagacg aacggctcat cgcgatcttt acgaataact cggcttaaca
     7081 aaaatccaga aacgaccggg atccaactct gttcaacatt gtcgtagtag caattttcgt
     7141 acttagactg acaattctgc tgagtcatat tcttcggaac atcggcataa aatgccgctt
     7201 tggcgttgtt ccagccacgc gcgcaaatgt cggcattatt accgtcatca atacaatcct
     7261 gcactgtcga ataaaacgtt ccgtcgccgt cgttatcgac atcgccactt tcactccatc
     7321 ctttgactat taaaaaaacg gcaccgccca ttagcaccaa cgttaagtat ttttgtgcgt
     7381 agcgattgcg gcgcggctca aaaggattat gcggtcttcc aatgcgactg atcgctccgt
     7441 gaccaatttt actgttattt cttgatttgc gttttctggc catgatcgtc cattaccatg
     7501 tcatacaagc tgcgttaaaa ataccaccag ccagtgcggc ggttcccata aacattcctg
     7561 ctgcggtatt gtgattaata attttttcgc ttaatgcggg catataaagt ctgacaccag
     7621 caaaaacgag taactgaatc accagtgcaa tcccgcccca ggcgaaatag tctggaatac
     7681 tcaccgcatt gattgccgca ctggataagg ggatcacgta acccaacaat gtaccgctga
     7741 atgccagtga cgccgcggta ttattgtttt tgattaattg ccattcgttg tgcggtgtaa
     7801 ttttggagta gataaacaga aaaataatca ccatagccac gccaataaaa aaataggcac
     7861 taaaggcaag aagtgaatcc agtatatgca ttgtaaacgt ccttgtaatt aaccaataat
     7921 atgaagtgat gtcagcggga tatcgacacc caacgcgcgc gaaaataacc actctggctc
     7981 gccgagatcg ttaaaagatt cctcaccatt taacagcaaa tattcgtaag tatcttcggt
     8041 tacttggcgt tggtagccca tggtaaaatt atggacctcc catttggcat ggttttgatt
     8101 ttctactttt tccagcatgt agacgggttc gatatttccc ggttcttcgc tgttaaaaaa
     8161 tcgctgccag cgtttttcct gccagtttaa gatcattgcc cccatcactt tggcgttgat
     8221 cgcctcgcgc cagtgactct ctttactgat accgtagctc tcttcataga caaagagctt
     8281 gatatcatca atgtcatcaa tatcttcgcc gccggtggta ttgatttgca gaaattcatc
     8341 gcctgaagtg tagtagcgga aaatctgact accgccgccc agatcgatgt ggctgacagc
     8401 ggctaccgta aattcctcac ccggcagcgc aatcagcaat tcatcttcca gcaaacgaaa
     8461 cgctaacgtg tcgagcgtaa agccactatt gagatgaagt cccagcggac cgcgagcgat
     8521 agcaggctta ttatccttgc caaacagacg ctggaaaaaa ccagacatat tttatcctta
     8581 aaatagttgc cagcctttta gcgaggctgg caaaaaaatt actcgccctg ttggcgttgc
     8641 agtcttgcta atacatcctg ggcgctactt ttattgctac cgccaattcc ggcttccgcc
     8701 agcttttcat caagatcgcg accgtctgcg actttttcca actgtgcagc agcatccagg
     8761 cgagcctgac gttcggcctg acgcgtttgc aggcgtttta aggattctgc cgctgtcgaa
     8821 acgctggaag atgcgccaac ggtagaggtt gttacagcct gttgtgcacg ctgcatggct
     8881 tcagtggctt taacgacttc catttgctgc tcaaactgag cgatacgctg cgctgtcgct
     8941 gtaactgctt tttccacgcc atcacgggag acttccagat tcgacaacac ttgctcttca
     9001 gcggtaatga gattctcaag gcgcgcgatt tcttcagcaa cttcgttaat caacgacgga
     9061 ttaacgttct tgcttaacgc ttccagcgca cgggcttcca gactggcttt gcgctcacgt
     9121 aaatctttca gcttatcgtg acttaatttc acccgcgcca gcagatcaac gcgagatttt
     9181 ccggctttat cgagttcagc tttagcgtca cgaatatgct gttccagcat gcgcacgccc
     9241 tgggtttctt caatggattc ttccgcctgg gagataaacg atttccccag cgtaaataaa
     9301 ctttttaaaa ttcccataaa tacccgtcct tgtaattagt gtgaatattc ttctgtaatt
     9361 tcagccagat ccaatgcgtt atctacctgc gaggttatct caagcaggat atcttcaaga
     9421 gaagatttaa gcgataacgc cccgaaaaca atgtaatact cttcctgttg cacgctggag
     9481 attcctaccg atgacaacgg catcatcttc tgatttctta ataagaaggt attaaattca
     9541 tctggattgc tgatactact taccggacaa ataaaggttt cgataatcat ttggcgggaa
     9601 gtaaaaagaa tattgatttg caaatcgcca taatcattca ttttaataat taatgcgctc
     9661 tcgctatttg tcacatcaat attttgttca ggtacagttt gcagcgccgt cgccagcgcc
//
LOCUS       DAZFXT010000162         9681 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.162, whole genome shotgun sequence.
ACCESSION   DAZFXT010000162 DAZFXT010000000
VERSION     DAZFXT010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.162"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..645)
                     /locus_tag="ACHKW0_004318"
     CDS             complement(<1..645)
                     /locus_tag="ACHKW0_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477038.1"
                     /translation="MAVNSNGSNNRVAGRDFHEKNIQIERYDGSHTVNIAIPSNNDDD
                     DRPLLKAQRKELNSLVAAIAEASNTEAFIIWQKVHAEIGVAGIDDMTVNQYKTAESFL
                     HAMLERCKDHDACKALVSLLLRNSEDCGLRQKLLRYCHINFGTGRLNDLTRSQLQSAL
                     SWLEQQSASSHTESSTLPEVRLRASELIRLYPKEIIFFICVGVLVGGVISRAFFN"
     gene            complement(661..1065)
                     /locus_tag="ACHKW0_004319"
     CDS             complement(661..1065)
                     /locus_tag="ACHKW0_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7477039.1"
                     /translation="MKNDIGQRLREERERLGLSQVAMSDIGGVKKLTQLRYEKGDSFP
                     DAAYLAALSRFGLDVQYVVLGIHSPETYNDDEQELITRFRAASLDVKNAVIGALKGAI
                     SEKETQPSGRELNISGGNNRIAGRDYNETKGR"
     gene            complement(1156..1293)
                     /locus_tag="ACHKW0_004320"
     CDS             complement(1156..1293)
                     /locus_tag="ACHKW0_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477040.1"
                     /translation="MRAMLEDERCSLAAISSMTARSSRDSRSASLGPVAVLRGMRDLV
                     S"
     gene            1365..1568
                     /locus_tag="ACHKW0_004321"
     CDS             1365..1568
                     /locus_tag="ACHKW0_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7477041.1"
                     /translation="MSGITININVNAPYVSLQKYAEITGIPLNTCKKMLADGRIIIRP
                     KRAKMEKPEVNLVAMLKDALANS"
     gene            1590..1940
                     /locus_tag="ACHKW0_004322"
     CDS             1590..1940
                     /locus_tag="ACHKW0_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4761 family protein"
                     /protein_id="HFO7477042.1"
                     /translation="MKKNANNPYSKFRNGVERHVHHVATSASRSNSRYNLNETHATPD
                     GHAVKQIGEHAWLIEKAGIVVHKCPRNPFTGNRIFALNCGDNHFGQDFTLYEALRTVD
                     RLLRGQSFIKQADL"
     gene            1951..2229
                     /locus_tag="ACHKW0_004323"
     CDS             1951..2229
                     /locus_tag="ACHKW0_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477043.1"
                     /translation="MTKEHAQGVFIRFIDFRGELLLRASAIDGVVPSEKNAATYVYLN
                     GTRLTVELPYQTVHEIISEAEKARQVNGDKPYIEIICMDSEAEIQKAD"
     gene            2241..2483
                     /locus_tag="ACHKW0_004324"
     CDS             2241..2483
                     /locus_tag="ACHKW0_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4754 family protein"
                     /protein_id="HFO7477044.1"
                     /translation="MDKEYKTLINKALERFYFRLSASGVHAERAARDSLTRAIRSLYD
                     VAFYADDLDALNELSELICAAECGEHIEPYKLGNIA"
     gene            2480..2593
                     /locus_tag="ACHKW0_004325"
     CDS             2480..2593
                     /locus_tag="ACHKW0_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477045.1"
                     /translation="MSIFISWLVLIISVACAIGIMRIINSVKKIERFFSDE"
     gene            2687..3097
                     /locus_tag="ACHKW0_004326"
     CDS             2687..3097
                     /locus_tag="ACHKW0_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477046.1"
                     /translation="MHAKEEGIIRALKEISKMESEVAKKAVANAHMDVATHTMIVAKV
                     TAEAAKIIEEQGVELALLKTKPVTGLDLSDTGRLIYTIGSEPQRYTIIAGLQNKYLIT
                     PHPIRESTLLTNLRLIERSQVAFIDDARHTVFNA"
     gene            3121..3324
                     /locus_tag="ACHKW0_004327"
     CDS             3121..3324
                     /locus_tag="ACHKW0_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477047.1"
                     /translation="MAIKHFPVVRFTSRGREYEVDERLITTIDKHRSEKDAHHIYLTD
                     GTYFCATNVARVNLIRQVQEPRR"
     gene            3321..3587
                     /locus_tag="ACHKW0_004328"
     CDS             3321..3587
                     /locus_tag="ACHKW0_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HFO7477048.1"
                     /translation="MTILDYIATHPGCSGGEIAAALNTPTTAINAELRQLWRGGLVIR
                     TNRSTGGRARKTGGQASYHVNPMPFGCSNPLTHMFNQLLKEART"
     gene            3584..3883
                     /locus_tag="ACHKW0_004329"
     CDS             3584..3883
                     /locus_tag="ACHKW0_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477049.1"
                     /translation="MSTINHQKLRELAFALQRMATPQKLLAFRAMLSPSAVLALLDEL
                     EHARTTAPAIRLTLHHEIADFCATLEAPGEPETPEAIQQELLQRIDKVFDFFLNQ"
     gene            4206..4418
                     /locus_tag="ACHKW0_004330"
     CDS             4206..4418
                     /locus_tag="ACHKW0_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="derepression protein"
                     /protein_id="HFO7477050.1"
                     /translation="MANRKQQRAYAARRHIQTEINRRLFRASRVAQIMHINMLHERSH
                     ALSNIYSASVFSYLADDLRELQQLFQ"
     gene            4509..4898
                     /locus_tag="ACHKW0_004331"
     CDS             4509..4898
                     /locus_tag="ACHKW0_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001534315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HFO7477051.1"
                     /translation="MIDAHDFTRWVRTQDTRLAPVLQGLFDLYIRGRDNRARTTKPEN
                     ADTLYFTVDDCYRVDFTPHGLALHCLTPHGESLLAYYDSPASVFAAMLAHRTAGGCAS
                     LSEYTAEFNRLSALFSQEWQRVTGYQP"
     gene            4895..7735
                     /locus_tag="ACHKW0_004332"
     CDS             4895..7735
                     /locus_tag="ACHKW0_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HFO7477052.1"
                     /translation="MSEFAWSWNEPRPAIDPARFTEHRQETETDLQRAIRYYLEADKK
                     AREEQEAKEEAFFAQSAMGKKLMASLEEAGQREKLAQSIISKRRATEQDPVARAFATL
                     KALPVYLREPLSRHLSFLRKKQEADRQKGKKSWQAERYARGTLRKIFERLDRTDSRWL
                     TPGYRSLAGRERLDDLLYLPQLNKHQIQTLATMTAAMFSSTFEKLCDGFGATDGELTM
                     DVTLKAYQMLARMALHLHAMPPHYDALTTDKDRRNEPDTELLPGAILRLTCAEWWKRK
                     LWLLRCEWREEQLRAACLVSRKTSPYLSQDALSEFRAQREKTRDFLKSFMLENEDGFT
                     IDLETVYYAGVSNPVHRKAEMMATMKGLELLAEARGDKAVFLTVTCPSKYHATTENGH
                     PNPKWNGATMRDSSDYLVNTFFAAVRKKLNRDGLRWYGIRTVEPHHDGTVHWHMMVFA
                     HPEEIDTIVSHTRDIAIQEDRHELGDDITPRFKAEYVDGSKGTPTSYIATYIGKNLDS
                     RAVDGIDPKTGKPRVDHETGKSMAESVERAIGWARLHRVRQFQFFGIPSRQVWRELRR
                     LASQMARNPEGPQRLKDDAMDAVLAAADAGCFATYIEKQGGVLVPRKDYLIRTAYDLA
                     DELNDYGEQSVQIYGIWSPLIGESSRVCTHPDNWKLVRRKPEAEDSARENGFDLQGGP
                     AAPWTRGNNCPRVQETDNNGTEQPEERPAPWPQLPDGVDVDEWVRSLKRHERRALMRS
                     LRDKQAKNSSDEMQSWTQSRKQQRPLPDNHELLAREWRESAESLGLHIGEQQMQHLLR
                     GGSLYVDGSIIAPQGFEIVRKPDTRPDSRITQLWQRLSRNHGVSSTEIRHNPVASYLA
                     QLGASDPEAATRLASTLQQDQNTMKTPVTVLSDMLRAIRDAEHAQRISETTERARRKA
                     DLLRGGLTSGNKKQTETGFTNPVNEQKTRRDI"
     gene            7812..8771
                     /locus_tag="ACHKW0_004333"
     CDS             7812..8771
                     /locus_tag="ACHKW0_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HFO7477053.1"
                     /translation="MKICIDDGSTNIKLAWTENSERRNAISPNSFKSEWSAPFGGSQP
                     ANYMLDGVRYGFDPVSDRFVQTTDTQYQYSDVNVIAIHHALVKSGITPQEVDVVVTLP
                     LSEYFDTNAQPDMANINRKKANVMRPVEYQNGEAFTIRNVRVMPESIPAGFKALADMS
                     PFESLLIVDLGGTTLDVAKVQGQLAGISQVFCDPHVGVSLMADAVLSVMATNGMRTSH
                     HIANTIIEHRHDEAWLRQHIHNDAHYASLMAVIREKEETLKQRVIRALAGFSGYGRVM
                     VVGGGAEIVAPAIREACGVNATFIADGVPQFALVNGLYAMDKE"
     gene            8776..9087
                     /locus_tag="ACHKW0_004334"
     CDS             8776..9087
                     /locus_tag="ACHKW0_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012907691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HFO7477054.1"
                     /translation="MTTPTRRISFYLKPAAVKNEGEACAWLDSLTPEARKSGQRVAFL
                     AGLALLKMNPAEAYRLAAWADDEALSVTQTRTERPASQPVSTAQITSQMAGNIRALFP
                     E"
     gene            9151..9521
                     /locus_tag="ACHKW0_004335"
                     /pseudo
     CDS             9151..9521
                     /locus_tag="ACHKW0_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289969.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
BASE COUNT         2677 a         2595 c         2345 g         2064 t
ORIGIN      
        1 attaaaaaac gccctagaaa tgacaccgcc taccaaaacc cctacgcaga taaagaatat
       61 tatttctttt ggataaagtc ggattaattc tgaagcacga agtcggactt ctggtaaggt
      121 cgaactctct gtgtggcttg atgccgattg ttgctctaac cacgacaatg cagactgtaa
      181 ctgagaacga gtaagatcgt ttaaacgtcc tgtaccgaaa ttgatatggc aataccgcag
      241 aagtttttgt cgaagtccac agtcttcact gttacgtagt aataaactta caagagcctt
      301 acaggcatca tgatctttac atcgctcaag cattgcatgc agaaaactct ccgctgtttt
      361 atattgattt actgtcatat catcaatacc agctacacca atctccgcat gtactttttg
      421 ccaaataata aacgcttcag tattgctagc ttctgcaata gcagcaacca agctatttag
      481 ctccttacgc tgagccttaa gcaaagggcg atcgtcatca tcattattcg aagggattgc
      541 gatattgaca gtatgagaac catcatatcg ctctatctga atattctttt cgtgaaaatc
      601 acgcccagca acgcgattgt ttgaaccgtt tgagttgacg gccatgtcac ctccctacta
      661 tcacctaccc ttagtttcgt tatagtcacg accagcgata cggttattac caccagaaat
      721 atttaactca cgtcctgatg gctgagtttc cttttcactg atcgcacctt ttaaagcccc
      781 aatcaccgcg tttttcacat ctaacgaagc tgctcgaaag cgagtaatca actcctgctc
      841 atcatcgtta taagtttcag gtgagtgaat tcccaacaca acatactgaa catcaaggcc
      901 aaaacgagac agcgctgcca aatacgcagc atcaggaaag ctatctcctt tctcatatct
      961 aagctgagtt agctttttga ctccaccaat gtcgctcatg gcaacttgac taagtcccaa
     1021 tctttccctt tcctcacgca accgctgacc aatatcattt ttcatacaaa aaccttgata
     1081 ggtatctttt tttgatacca aaatgatttc acgagctatt agatgatcac aatataccac
     1141 tatgaaacaa gttcttcacg ataccagatc acgcattccg cgtaacaccg ccacaggtcc
     1201 aagactggca cttcggctgt cccttgagga gcgagccgtc attgatgaaa tggcagctaa
     1261 agaacaacgc tcatcctcta acatggcgcg catgatcttc cttcgcggcc tagagctaac
     1321 ccagaaagaa caaaacaaat cttcctgatc aggaggctag tgggatgtca ggtataacca
     1381 tcaatatcaa tgtgaatgcc ccctatgtat ccctgcagaa atatgcagag ataacaggta
     1441 tccctcttaa tacgtgcaaa aagatgttgg ctgacggtcg aattattatc cgccccaaac
     1501 gcgccaaaat ggaaaagcct gaagtaaacc ttgtggcgat gttaaaagac gctttggcta
     1561 acagctaaaa caatgaacag agcaccatca tgaaaaaaaa cgctaataat ccatactcca
     1621 aatttcgtaa tggcgtagaa cgccatgtac accacgtcgc taccagtgca tcacgtagta
     1681 acagtcgcta taacctgaac gagacgcacg caacaccgga tggccacgct gtaaaacaaa
     1741 tcggcgagca tgcctggctg attgagaaag ctggaatcgt ggtccacaaa tgcccacgca
     1801 atccgtttac cggaaaccgc atttttgcat tgaactgcgg cgacaatcac ttcgggcagg
     1861 atttcacatt atacgaagca cttcgcacgg ttgatcgtct gcttcgcggg caaagtttta
     1921 ttaaacaggc tgatttataa caggtgcttt atgaccaaag agcatgcaca aggtgtattt
     1981 atccgtttta ttgattttcg cggtgaactg ttattacgcg catcagctat tgatggagtc
     2041 gtcccatcag aaaaaaatgc agctacttac gtttatctga acggcacgcg cctgaccgta
     2101 gaacttccgt accagactgt acacgaaatc attagcgaag ctgaaaaggc acgtcaggtt
     2161 aatggcgata aaccctatat cgaaattatc tgcatggatt cagaagctga aattcagaaa
     2221 gcagattaaa gggtgttgca atggacaaag aatataaaac actcatcaac aaagcacttg
     2281 agcgttttta ttttcgctta agcgcatcag gcgttcatgc tgaacgtgca gcccgtgact
     2341 cattgaccag ggcaatccgg agtctgtatg acgtggcttt ttacgctgat gatctggatg
     2401 cacttaacga actttccgag ctgatctgtg ccgcagaatg cggagaacat attgaaccgt
     2461 ataagctggg gaatatcgca tgagtatatt tatctcatgg cttgttctga ttatttcggt
     2521 ggcctgcgcc attgggatta tgcgaattat taattcagtg aaaaagatcg agcgtttttt
     2581 ctctgatgaa taacgataca aataaaacat caaattaaat aagaaaacgt gaaaaccatc
     2641 cgtattaacg gaggtattcg cacacgcaaa taacggagat acaaaaatgc acgcaaaaga
     2701 agaaggtatc atcagagcac tgaaagaaat ttcaaagatg gaaagcgaag tagcgaaaaa
     2761 agccgtggcc aatgctcaca tggacgtcgc aacccacaca atgatagtcg caaaagtcac
     2821 ggcagaggct gccaaaatca tcgaagaaca gggtgtggaa cttgcgcttc tcaaaactaa
     2881 accagtcacc ggactggatt tatctgacac cggacgcctt atttacacta ttggctcgga
     2941 gccacagcga tacaccatta tcgccggatt acagaacaaa tacctgatca ctcctcaccc
     3001 cataagggaa tcaacgcttc tgacaaatct ccgcctgata gagcgctctc aagttgcatt
     3061 cattgatgac gcccgacaca ccgtatttaa cgcatagggt tactggacaa agggggtgca
     3121 atggcaatta agcattttcc cgtcgttcgc tttacctcca gagggcgcga atacgaggtc
     3181 gacgaacgcc tgattaccac tatcgacaaa catcgttcgg aaaaggatgc acaccacatc
     3241 tacctcactg acggtactta cttctgcgcc accaacgtgg cgcgggtgaa tcttatccga
     3301 caggtacagg agccacgcag atgaccattc tggactacat cgctactcat ccgggttgta
     3361 gcggcggaga gatcgccgca gcactgaata ctccaaccac agccattaat gctgagttac
     3421 gccaactttg gcgcggcggc ttagtcatca gaacaaaccg cagcacaggt ggtcgcgctc
     3481 gcaaaactgg aggccaggct tcttaccacg taaacccgat gccgttcggg tgtagcaatc
     3541 cacttactca catgtttaac cagctactaa aggaagccag aacatgagca ccatcaacca
     3601 ccagaagcta cgcgaactgg catttgccct gcaacgaatg gcaacgcctc aaaaattact
     3661 ggcatttcgc gcaatgctct cgccgtctgc tgtgctggca ctgctggatg agctggagca
     3721 cgccagaacc acggctcctg ccattcgcct gacactccat catgaaatcg ctgatttctg
     3781 cgcgacgttg gaggcaccag gcgaaccgga aacgccggaa gcaatacagc aagagctgct
     3841 gcaacgcatt gacaaggttt ttgatttttt tctgaaccag taagaaacca gaacatgcac
     3901 acacaaaaaa accgcttgcc atgccgcaat cagtcaggtt acatttccgc tgcacctcat
     3961 aaaacgggtg ccgggattct caacccgata cagagcaaag cgcataaccg cgccagcggt
     4021 ttttttgtgc gtactgtatt gccacgtctt tttcgcgtca gaattatggc ggggcgtacg
     4081 gggccgactt cggtcgggcc ggattctttg ctctccggtg ttgagaaccc tgtacgtctc
     4141 gccaccccga gattctcaac tctggatggt gagctatttc tatcaccgag caaagaggcc
     4201 acaccatggc aaaccgcaaa caacagcgcg catacgctgc gcgtcgtcac atccagactg
     4261 aaatcaaccg tagacttttc cgcgcatcac gcgtcgcgca aatcatgcac atcaatatgc
     4321 tgcatgagcg cagccacgcg ctatcgaaca tctattccgc ctctgttttc agctatctgg
     4381 cggatgatct gcgcgagctg caacaactct tccagtagca aaacaaactc cattaattcc
     4441 tgttccgggc ctttcctgca ccttgcggcg ggaggccttc gcacatctgt agtaaagaga
     4501 attgcagcat gattgacgct catgacttca caagatgggt gcgcacacag gacacccgtc
     4561 tggctcccgt tcttcaggga ttatttgatc tctacatccg tggtcgtgac aacagagcac
     4621 gcaccacaaa accagagaat gcagacaccc tttatttcac agtagacgac tgctaccgcg
     4681 tggacttcac accacacggg ctggcgttgc actgcctgac accacacgga gaatcactgc
     4741 tggcgtatta cgactccccg gcctccgtat ttgcggcaat gctggcgcat cgcactgctg
     4801 gcgggtgtgc ctcgctgagt gaatacaccg ctgaatttaa ccgcctttct gccctcttct
     4861 cgcaggagtg gcagcgcgtg acaggatacc agccatgagt gagtttgcat ggagctggaa
     4921 tgaaccacgg ccagccattg atccggccag atttacggag cacaggcagg aaactgaaac
     4981 cgacctgcaa cgcgccatcc gttactacct tgaggcggac aaaaaggccc gggaagaaca
     5041 ggaagcgaag gaggaagcct ttttcgcaca atccgccatg ggtaaaaaac tcatggcatc
     5101 ccttgaggaa gccggacagc gtgaaaagct ggcacagagc atcatcagta agcgccgggc
     5161 aacagaacaa gacccggtgg cccgtgcctt tgccacactg aaggcgcttc ccgtttatct
     5221 gcgagaacct ctgagccgcc acctctcttt cctgcgcaag aagcaggaag ccgatcgtca
     5281 gaaaggcaaa aagagctggc aggcggaacg ctatgcacgc ggaaccctgc gcaaaatatt
     5341 cgaacgtctg gaccgcaccg acagccgctg gctgacaccg ggttatcgct cccttgccgg
     5401 acgcgaacgc ctggacgatt tgctttacct gccgcagctc aacaaacacc agatacagac
     5461 gctggccacc atgacggcgg cgatgttcag cagcaccttc gaaaaactct gcgatggctt
     5521 tggcgcgact gatggcgaac tgaccatgga tgtaacgctg aaggcgtatc agatgctggc
     5581 ccgcatggcg ttacacctgc acgccatgcc tccacattat gacgcactga caacagacaa
     5641 agaccggagg aacgaaccgg acacggagct gctgccgggc gcaatccttc gcctgacctg
     5701 tgcggaatgg tggaaacgca aactgtggct gttacgttgc gagtggagag aagaacaact
     5761 ccgcgccgcc tgtctggttt ccagaaaaac atcaccctat ctgagccagg acgcgttaag
     5821 cgagtttcgc gcacagcgcg agaaaacacg cgatttcctg aaaagtttta tgctggaaaa
     5881 cgaagacggg ttcacgattg atctcgaaac agtgtattac gcgggagtaa gtaacccggt
     5941 tcaccgtaag gcagaaatga tggccaccat gaaggggctg gaacttctgg ccgaagcccg
     6001 tggcgacaaa gcggtgtttc tgactgtcac ctgcccgtca aaataccacg ccacaacaga
     6061 gaacggtcat ccgaatccca aatggaacgg agccaccatg cgcgactcca gcgattacct
     6121 ggttaacacg ttttttgcgg cggtccgcaa gaaactgaac cgcgacggcc tgcgctggta
     6181 tggcatccgc acggtggagc ctcaccatga cggcaccgtg cactggcata tgatggtctt
     6241 tgctcatccg gaagaaatcg acaccattgt gtcccacacc cgcgatattg ccattcagga
     6301 agatcgtcac gagctgggtg atgatattac cccacgcttt aaggcggagt acgtcgacgg
     6361 ctcaaaaggt acgccaacca gctacatcgc cacctacatc ggaaagaacc tagacagccg
     6421 cgccgtggat ggcatcgacc cgaaaacggg caagccacgc gttgaccacg aaaccggaaa
     6481 atcaatggcc gagagcgtgg aacgcgccat cggctgggcg cgccttcacc gtgtccgcca
     6541 gttccagttc tttggtatcc cctcccgtca ggtgtggcgt gaactccgcc gccttgccag
     6601 ccagatggca cgcaacccgg aaggcccgca acggctgaag gatgacgcaa tggatgcggt
     6661 actcgctgcc gctgatgccg gatgttttgc cacctacata gagaaacagg gcggcgtact
     6721 tgttccacgc aaagactacc tgattcgcac cgcctacgac ctcgcagatg agctgaacga
     6781 ttacggcgaa cagagtgtac agatttacgg gatctggtcg ccgctcatcg gggaatcctc
     6841 ccgtgtgtgc acgcatccgg ataactggaa gctggtaaga cgtaaaccgg aagcggaaga
     6901 cagcgcccgc gaaaatggtt ttgaccttca gggcggccct gccgcccctt ggactcgtgg
     6961 caataactgt ccccgtgtac aggaaacgga caacaacggg acagaacagc cggaagaacg
     7021 gccagcaccg tggccgcagc ttcctgacgg cgttgatgtg gatgaatggg tgcgctcact
     7081 gaaacggcac gaacgccggg cgctgatgcg ttcgcttcgt gacaaacagg caaaaaacag
     7141 cagtgatgaa atgcagagct ggacacagag ccgcaaacag cagcggcctt tgcctgataa
     7201 ccacgaatta ctcgctagag aatggcggga gtctgctgaa tctctcggcc tgcatatcgg
     7261 tgaacaacaa atgcagcacc tgttacgggg cggcagtctg tacgttgacg gcagcatcat
     7321 tgcaccgcag ggatttgaaa ttgtacgcaa accggatacc cgcccggaca gccgaatcac
     7381 gcagctctgg cagcgcctga gccgtaatca cggtgtaagc agcacggaga tccgccataa
     7441 cccggtcgcc agctatctgg cacagctggg ggcatcagac cctgaagccg ccacacgcct
     7501 ggcatccaca cttcagcagg accagaacac catgaaaaca cccgttaccg tgctttctga
     7561 catgctgcgc gccatccgcg acgcagagca cgcacagaga atcagtgaaa ccactgaacg
     7621 tgcccgccgc aaagcagacc tgctgcgggg tggcctgacc agtggaaaca aaaaacagac
     7681 agaaacggga ttcacaaatc ccgtaaatga gcaaaaaacg cgccgcgata tatgaagcgc
     7741 acacaaaaca ggcaaaaacg ggatttcaga atcccgtaaa cgattaatta atcaacataa
     7801 ggaaaagcga catgaaaatt tgtatcgacg acggctccac caacatcaag ctggcatgga
     7861 ctgagaacag cgaacgccgc aacgccatca gcccgaacag cttcaagtcg gaatggtctg
     7921 cgccgttcgg tggctcgcag cctgcgaact acatgcttga tggcgtgcgc tatggttttg
     7981 atccggtcag cgatcgcttt gtccagacga ccgacacgca ataccagtac agcgatgtga
     8041 atgtaatcgc cattcaccac gcgctggtca aatcaggcat cacaccacag gaggtggatg
     8101 tggttgtcac cctgccactg agcgagtatt tcgacacaaa cgcacagccg gacatggcca
     8161 acatcaaccg caaaaaagcg aacgttatgc gcccggtgga gtaccagaac ggcgaagcat
     8221 tcactatccg taacgtgcgg gttatgcctg aatccattcc ggctggcttt aaggcactgg
     8281 ctgacatgag tccgtttgaa tccctgctga ttgtggattt aggcggaacc acgctggatg
     8341 tggcaaaggt ccagggacaa ctggcaggta tcagccaggt gttttgcgat ccacacgtag
     8401 gcgtttccct gatggccgat gccgtactgt cggtgatggc cactaacggt atgcgtacca
     8461 gtcaccacat cgccaatacc attatcgaac atcgccatga tgaagcctgg ttgcgccaac
     8521 acatccacaa tgacgcgcat tacgccagcc tgatggcggt tattcgtgaa aaggaagaaa
     8581 cactgaaaca acgcgtgatc cgcgcgctgg cgggtttttc gggttacgga cgggtgatgg
     8641 ttgtcggtgg cggggcggag attgtggcac ccgctatccg cgaagcctgc ggagttaatg
     8701 cgactttcat cgcggacggg gtgccacagt ttgctctggt taatgggctg tacgcaatgg
     8761 acaaggagta aaccaatgac gacaccaacc agacggataa gtttctatct gaagcccgcc
     8821 gccgtcaaga acgaaggcga agcatgcgcc tggctggaca gccttacacc agaagcccgc
     8881 aaaagcggcc aacgtgtggc ttttctggcc gggctggcac ttctgaaaat gaatccggca
     8941 gaggcttacc gactggctgc atgggctgat gatgaagcgt tatcagtgac acaaaccagg
     9001 acagaacgcc ccgcatcaca gccagtatca accgcacaga taaccagtca gatggccgga
     9061 aatatccggg cgttatttcc tgaataacac aacatcaggg cgagttcgcc ctgctctcca
     9121 cctcagaaca taaacaagga gaacgactta atgagcgaaa tcaactatca ggcattacgt
     9181 gaacgttatt cacctgtgcc agtaccgaaa tgccctattt gcggcgagga aatgtcaatt
     9241 cagcgaatat ctggagcaca ggttgtttat gcctgctccg gttatggtga tgatggagat
     9301 ttcaaaattg gccgaactct tgccgacgaa cattatgaaa aatcacacgt aacagtgttg
     9361 gatgtcggga tcctgaagta ttggcgctac ttgattggct ggaaaccaaa gacaaccgaa
     9421 tcgctgaact ggaaaaaatc gccactgact atgcacttaa attccagaaa gcacaggacg
     9481 cattaaagta cgccgctttg ctgcatagca ggacagcgca gctaaaatac taacttaaag
     9541 cggaagatta atacatggtg agtcggggta atttgttttt atcccaacaa ataaacaaaa
     9601 aagtggacta tctctgtagt atcttattca gcatcgtctc gtgcagtctg tgcatacgtc
     9661 acaacaacgc ccaatgcatt a
//
LOCUS       DAZFXT010000163         9615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.163, whole genome shotgun sequence.
ACCESSION   DAZFXT010000163 DAZFXT010000000
VERSION     DAZFXT010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.163"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..809
                     /locus_tag="ACHKW0_004336"
     CDS             <1..809
                     /locus_tag="ACHKW0_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7477055.1"
                     /translation="LTKRQFKVLLAILRKTYGWNKPMDRITDSQLSEITKLPVKRCNE
                     AKLELVRMNIIKQQGGMFGPNKNISEWCIPQNEGGSPKMRDIPQNEGKSPKTRDKTSL
                     KLGDCYPSKQGDTKDTITKEKRKDYSSENSGESSDQPENDLSVVKPDAAIQSGSKWGT
                     AEDLTAAEWMFDMVKTIAPSARKPNFAGWANDIRLMRERDGRNHRDMCVLFRWACQDN
                     FWSGNVLSPAKLRDKWTQLEINRNKQQAGVTASKPKLDLTNTDWIYGVDL"
     gene            806..1507
                     /locus_tag="ACHKW0_004337"
     CDS             806..1507
                     /locus_tag="ACHKW0_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7477056.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITSMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELTCMTARINRGETIPEPVKQLPVMGGRPLNRVQALAK
                     IAEIKAKFGLKGASV"
     gene            1504..1794
                     /locus_tag="ACHKW0_004338"
     CDS             1504..1794
                     /locus_tag="ACHKW0_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO7477057.1"
                     /translation="MTGKEAIIHYLGTHKNFCAQDVSAVTGATVTSINQAAAKMARAG
                     ILVIDGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            1865..2143
                     /locus_tag="ACHKW0_004339"
     CDS             1865..2143
                     /locus_tag="ACHKW0_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477058.1"
                     /translation="MNDSYRQFENWWSKDKSQFTGDDELKEFAWVIWQASRSAIELDI
                     DWPESNDDFWKDGEEGAYAMGYEDGRDKTVIAVMKAIRAAGIKEKNFD"
     gene            2276..2491
                     /locus_tag="ACHKW0_004340"
     CDS             2276..2491
                     /locus_tag="ACHKW0_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477059.1"
                     /translation="MSTIAELVRANFREELVRWYRYRSSSSLPLDELYEHSPAARRYP
                     RDRVLRRLFKLNNEFQRNRIIRSLDLK"
     gene            2502..2738
                     /locus_tag="ACHKW0_004341"
     CDS             2502..2738
                     /locus_tag="ACHKW0_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lar family restriction alleviation protein"
                     /protein_id="HFO7477060.1"
                     /translation="MSDLSLTQPKLKECPFCGGNARLWVEAGINIDVWGYAECDLCEA
                     RGAWAPSVAAAAEKWNRRAGDEANLSASQRSNQK"
     gene            2695..3141
                     /locus_tag="ACHKW0_004342"
     CDS             2695..3141
                     /locus_tag="ACHKW0_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7477061.1"
                     /translation="MKQTFLLRNEAIRNNAIDAILSLPIDDKSPHEVHVKEPKRSKAQ
                     NDRMWPMLNDVSRQVLWHGQRLAPEDWKDLFTALWLKTKKLEQRSVPGIDGGVVMLGV
                     RTSKMRKASMTELIEIMFWFGSERNVRWSDDSRREYEWSQRKGRAA"
     gene            3138..3665
                     /locus_tag="ACHKW0_004343"
     CDS             3138..3665
                     /locus_tag="ACHKW0_004343"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7477062.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            3662..3844
                     /locus_tag="ACHKW0_004344"
     CDS             3662..3844
                     /locus_tag="ACHKW0_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7477063.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARKTR"
     gene            4119..4853
                     /locus_tag="ACHKW0_004345"
     CDS             4119..4853
                     /locus_tag="ACHKW0_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor Ant"
                     /protein_id="HFO7477064.1"
                     /translation="MTTQISVETLSPITHNQIPVITTELLAQLYGTEPVRIRQNHHEN
                     KVRFVEGKHFFKVVGNDLKELRVALNYSQNLRVTLSNSQNLQPSLRGLQISPKARSLI
                     LWTERGAARHAKMLETDQAWDVLEKLEDCYFSQCEKNTGKQEKKLNGLSAKETDSLVW
                     LWDYANRSQALFRELYPALKLIQSGYSGICHDYGYEFSYIIGRARGVLINHTRDIDIY
                     EPDGPTNLLAWERLKNKELPPSLHRY"
     gene            4928..5650
                     /locus_tag="ACHKW0_004346"
     CDS             4928..5650
                     /locus_tag="ACHKW0_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HFO7477065.1"
                     /translation="MNELINSNAIKMTSIEIAELVGSRHDKVKQSIERLAVRGVIRNP
                     PMVVFEKINNLGLLRGVEAYVFEGEQGKRDSIIVVAQLSPEFTARLVDRWRELEGATA
                     KIPQTFSEALRLAADLEDQKAELEKQLALAAPKVEFADRVGEASGILIGNFAKVVGIG
                     PNKLFAWMRDHKILIASGSRRNVPMQEYMDRGYFTVKETAVNTNHGIQISFTTKITGR
                     GQQWLTRKLLDNGMLKVTREAA"
     gene            5650..6255
                     /locus_tag="ACHKW0_004347"
     CDS             5650..6255
                     /locus_tag="ACHKW0_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7477066.1"
                     /translation="MAKPARRKCKICKEWFHPAFSNQWWCCPEHGTQLALERRSKERE
                     KAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCISC
                     GTLTSAQWDAGHYRTTAATPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIGQE
                     AVDEIESNHNRHRWTVEECRAIKAKYQQKLKDLRNSRSEAA"
     gene            6252..6446
                     /locus_tag="ACHKW0_004348"
     CDS             6252..6446
                     /locus_tag="ACHKW0_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO7477067.1"
                     /translation="MTFTVKTIPDMLLEAYGNQTEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            6439..6873
                     /locus_tag="ACHKW0_004349"
     CDS             6439..6873
                     /locus_tag="ACHKW0_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7477068.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            7380..8327
                     /gene="stxA1a"
                     /locus_tag="ACHKW0_004350"
     CDS             7380..8327
                     /gene="stxA1a"
                     /locus_tag="ACHKW0_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO7477069.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            8337..8606
                     /gene="stxB1a"
                     /locus_tag="ACHKW0_004351"
     CDS             8337..8606
                     /gene="stxB1a"
                     /locus_tag="ACHKW0_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO7477070.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
     gene            9106..>9615
                     /locus_tag="ACHKW0_004352"
     CDS             9106..>9615
                     /locus_tag="ACHKW0_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7477071.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVAVTGNEGKAVAVSATSDPEYYFVVVLAGQSNGMSYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
BASE COUNT         2702 a         2095 c         2552 g         2266 t
ORIGIN      
        1 atctgaccaa gcgacagttt aaagtgctgc ttgccattct gcgtaaaacc tatgggtgga
       61 ataaaccaat ggacagaatc accgattctc aacttagcga gattacaaag ttacctgtca
      121 aacggtgcaa tgaagccaag ttagaactcg tcagaatgaa tattatcaag cagcaaggcg
      181 gcatgtttgg accaaataaa aacatctcag aatggtgcat ccctcaaaac gagggaggtt
      241 cccctaaaat gagggacatc cctcaaaacg agggaaaatc ccctaaaacg agggataaaa
      301 catccctcaa attaggggat tgctatccct caaaacaggg ggacacaaaa gacactatta
      361 caaaagaaaa aagaaaagat tattcgtccg agaattctgg cgaatcctct gaccagccag
      421 aaaacgatct ttctgtggtt aaaccggatg ctgcaattca gagcggcagc aagtggggaa
      481 cagcagaaga cctgaccgcc gcagagtgga tgtttgacat ggtgaagact atcgcgccat
      541 cagccagaaa accgaatttt gctgggtggg ctaacgatat ccgcctgatg cgtgaacgtg
      601 acggacgtaa ccaccgcgat atgtgtgtgc ttttccgctg ggcctgccag gacaacttct
      661 ggtccggtaa cgtgctgagt ccggccaaac tccgcgacaa gtggacccag ctcgaaatca
      721 accgtaacaa gcaacaggca ggcgtgacag ccagcaaacc aaaactcgac ctgacaaaca
      781 ctgactggat ttacggggtg gatttatgaa aaacatcgcc gcacagatgg ttaactttga
      841 ccgtgagcag atgcgtcgaa tcgccaacaa catgccggaa cagtacgacg aaaagccgca
      901 ggtacaacag gtagcgcaga tcatcaacgg tgtgttcagc cagttactgg caactttccc
      961 ggcgagtctg gctaaccggg accagaacga actgaatgaa atccgccgcc agtgggttct
     1021 ggctttccgg gaaaacggga tcacctcgat ggaacaggtt aacgcaggaa tgcgcgtagc
     1081 ccgtcggcag aatcgaccat ttcttccatc acccgggcag tttgttgcat ggtgccggga
     1141 agaagcatcc gttatcgccg gactgccaaa cgtcagcgag ctggttgata tggtttacga
     1201 gtattgccgg aagcgtggcc tgtatccgga tgcagagtct tatccgtgga aatcaaacgc
     1261 gcactactgg ctggttacca acctgtacca gaacatgcgg gccaatgcgc tgactgacgc
     1321 ggaattacgg cgcaaggctg ccgatgaact gacctgtatg acagcacgaa ttaaccgtgg
     1381 tgagacgata cctgaaccag taaaacaact tcctgtcatg ggcggcagac ctctaaatcg
     1441 tgttcaggcg ctggcgaaga tcgcagaaat taaagctaag ttcggactga aaggagcaag
     1501 tgtatgacgg gcaaagaggc aattattcat tacctcggga cgcataagaa cttctgtgca
     1561 caggacgttt ccgcggtaac aggcgcaacc gtaaccagca taaatcaggc tgcggctaaa
     1621 atggcgcggg caggaatcct ggtcattgat ggtaaggtct ggcgaacggt gtattaccgg
     1681 tttgctacca gggaagaacg ggaaggaaag atgagcacga acctgatttt taaggagtgt
     1741 cgccagagtg cagcgatgaa acgggtattg gcggtatatg gagttaaaag atgaccatct
     1801 acatcactga gctaataaca ggcctgctgg taatcgcagg cctttttatt tgggggagag
     1861 ggaagtgaac gatagctacc gacagtttga aaactggtgg tcaaaagaca aaagccagtt
     1921 cacgggagac gatgaattaa aagagtttgc ctgggtgata tggcaggcat cgcgctctgc
     1981 tattgaactg gatatcgact ggcccgaatc gaatgacgac ttttggaaag atggtgaaga
     2041 aggtgcttat gcgatgggtt atgaggatgg gcgtgacaaa acggtaattg cagtaatgaa
     2101 agccatcagg gccgcaggaa tcaaagaaaa gaatttcgat taagcaaata tcacttcaat
     2161 aaatcgcttt taaggcatca caatcgctct gtagcgaggt aaacgcgtgc aaggcatgcc
     2221 aataagcagc gagaatgaaa aatgcgtcag aatgcgtttg aggaggtttt aagaaatgag
     2281 tacgatagct gagcttgtca gggctaattt tcgtgaggag ttggtgcgtt ggtatcggta
     2341 tcgttcatcg tccagtttgc cgcttgatga gttgtatgag cattcacctg ccgcacgacg
     2401 ctatccgcgt gaccgtgttc ttcgacggtt gttcaaactc aacaatgagt ttcagcgcaa
     2461 cagaattatc cggagtctgg atttaaagtg aaggagtgag catgagcgac ctatcattaa
     2521 cccagccaaa gctaaaagaa tgtccgtttt gcggcggtaa tgctcgtctg tgggttgagg
     2581 ccggaataaa tattgatgtg tggggctatg cagaatgtga cctctgtgaa gccagggggg
     2641 catgggcacc atcagttgct gcggcggctg aaaaatggaa ccggagagca ggagatgaag
     2701 caaacctttc tgcttcgcaa cgaagcaatc agaaataacg ccatagacgc cattctctca
     2761 ctacccatcg acgacaagtc accccacgaa gtccacgtta aagaacccaa gcgcagcaaa
     2821 gcgcagaatg accgcatgtg gccgatgctg aacgatgttt cgcgtcaggt gctatggcat
     2881 ggtcaacggc tggcgccgga agactggaaa gacctgttca ctgccctgtg gcttaagacc
     2941 aaaaaactgg agcaacgaag tgtgcctggt atcgacggtg gcgttgtcat gcttggcgtg
     3001 cgtaccagca aaatgcggaa ggccagcatg actgagctta tcgaaatcat gttctggttc
     3061 ggctcagagc gcaacgtgcg gtggagtgat gactcccggc gagagtatga atggtcacaa
     3121 cgaaaaggta gggctgcatg actatcaaat caaatacgcc agcacacgac aaggactgct
     3181 ggcaaacgcc gctttggctt tttgatgcac tggatattga gtttggattc tggctggatt
     3241 cggcagcgag cgacaaaaat gctctgtgcg ctcactggct aactgaggcc gacgacgcgc
     3301 tcaattctga gtgggtaagc cacggtgcaa tctggaataa cccaccgtac agcaatatca
     3361 ggccgtgggt ggaaaaagcc gctgagcagt gcatacaaca gcgacagacg gtagttatgc
     3421 ttgtgccaga ggatatgtca gtcggatggt tcagcaaggc tctggagagt gttgacgaag
     3481 ttcgcatcat cactgatgga cggattaatt ttatcgaacc atcgacaggg ctggagaaga
     3541 agggaaacag caaaggctcc atgctgctga tttggcgacc gttcatcagt cctcgacgaa
     3601 tgtttactac tgtatccaaa gcggcattga tggcgatcgg gcagggcgtc aggagggcgg
     3661 catgaggcga cagcgacgaa gtatcaccga catcatctgc gaaaactgca aataccttcc
     3721 aacgaaacgc tccagaaata aacgcaagcc aatcccaaaa gaatctgacg taaaaacctt
     3781 caattacacg gctcacctgt gggatatccg gtggctaaga catcgtgcga ggaaaacaag
     3841 gtgattgacg cgatgattta ttcggggcta tattcctcac gcgccagcaa aatctggcgt
     3901 cgggattggc gtcctggata gagaccgcga cagatacacg ccgcgagcgt gttttttatt
     3961 gtcgtatgca cgcgcacatc tgaattatgg tgggctgtgt gggggcggag agatccgcgc
     4021 cggtcggttt cccggttacg ccaaccctgc acagttcacc accagacgat tggcgtcgtc
     4081 ggtggtgagt tattaagaaa ccaccagagg gcgtcattat gacaactcaa atttctgttg
     4141 aaactctctc cccgatcacc cataaccaga ttcctgttat taccaccgaa cttttggcgc
     4201 agctttacgg cactgagccg gtgcgtattc gccagaatca tcatgagaac aaagtacgct
     4261 tcgttgaagg gaaacacttt ttcaaagttg ttggtaatga ccttaaagaa ttgcgggtag
     4321 ctttaaacta ctcacaaaat ttgcgggtta ctttaagtaa ctcacaaaat ttgcaaccat
     4381 ctttaagagg gttacaaatt tccccgaaag cccgctccct catactctgg acagaacgag
     4441 gcgcagcccg tcacgcaaaa atgctcgaaa ctgatcaggc gtgggatgtg ttagaaaaac
     4501 tggaagactg ctatttcagc cagtgcgaga aaaatactgg caaacaagag aagaagctca
     4561 acgggctttc cgcaaaagaa acagacagcc ttgtatggct gtgggattat gccaaccgct
     4621 cacaggcatt gttccgtgag ttgtatcccg cattaaaact gattcagtct ggctattccg
     4681 gcatatgcca cgactacggc tatgagttct cgtatatcat cgggagggcg aggggcgttt
     4741 taattaatca cacgcgggat atagatattt atgagcctga cgggccgacg aaccttctgg
     4801 catgggaaag gcttaagaac aaagagttgc cgccttcact gcatcgctac tgacaattga
     4861 caacttaaca aacccagctt cggctgggtt ttttattgct gaattttcaa tgtgagagga
     4921 catgacaatg aatgagctga taaatagcaa tgccatcaaa atgacaagca ttgaaatcgc
     4981 tgagttggtg ggaagccgtc atgacaaggt gaaacaatcc attgaacgac tggcggttcg
     5041 aggtgtgatc cgaaatcccc caatggtggt tttcgaaaaa atcaataact taggattact
     5101 tcgtggcgta gaggcttacg tttttgaggg cgaacaaggt aagcgcgaca gcattattgt
     5161 cgttgcccag ttgtcgccgg aattcaccgc tcgccttgtt gaccgctggc gagaactcga
     5221 aggggcaacc gcgaaaatac cacaaacctt ttctgaggca ttgcgccttg cggccgacct
     5281 tgaagaccag aaggctgaac tggagaaaca gcttgctctc gcagcaccta aagttgagtt
     5341 tgccgatcgc gttggcgagg ccagcggaat tttgattgga aactttgcaa aggttgttgg
     5401 tattggtcca aacaaactgt ttgcgtggat gcgcgatcac aaaatcctta ttgcttcagg
     5461 ctcccggcgc aatgtgccaa tgcaggaata tatggatcgt ggctatttca cagtgaaaga
     5521 aacagcggtc aatacaaatc acggaataca gatatcgttc accacaaaaa tcaccgggcg
     5581 tggtcaacag tggctgacca gaaagctgct cgataacgga atgctgaaag taacaaggga
     5641 ggctgcttaa tggctaaacc agcgcgaagg aaatgcaaaa tatgcaagga atggtttcac
     5701 ccggcattct caaatcagtg gtggtgctgc ccggaacacg gaactcaatt agcactcgaa
     5761 cgacgaagta aagaacgcga aaaagcggaa aaagcagcag agaagaaacg acgacgagag
     5821 gagcagaaac agaaagataa actgaagatt cgaaaactcg ccttaaagcc ccgcagttac
     5881 tggattaaac aagcccaaca agccgtaaac gccttcatca gagaaagaga ccgcgactta
     5941 ccatgtatct cgtgcggaac gctcacgtct gctcagtggg atgccggaca ttaccggaca
     6001 actgctgcga cacctcaact ccgatttgat gaacgcaata ttcacaagca atgcgtggtg
     6061 tgcaaccagc acaaaagcgg aaatctcgtt ccgtatcgcg tcgaactgat taaccgcatc
     6121 gggcaggaag cagtagacga aatcgaatcg aaccataacc gccatcgctg gactgtcgaa
     6181 gaatgcaggg cgatcaaggc gaagtatcag cagaaactta aagacctgcg aaacagcaga
     6241 agtgaggccg catgacgttc acagtaaaaa ccattcctga catgctcctt gaggcatatg
     6301 gaaatcagac cgaggtagcc cgaatactga actgcaatcg tgccacagtc agaaaataca
     6361 ttggcgataa agaagggaaa aagcacgccg tcgtcaatgg cgtccttatg gttcatcgcg
     6421 gatggggtaa agatactgat gcgtgatatc cggcaggttc ttgagcgctg gggggcatgg
     6481 gcggcaaata accatgagga tgttacatgg tcgcccattg ctgccggatt taagggactg
     6541 atccccgaaa aagtaaaatc acgtccacag tgttgtgacg atgacgcgat gattatatgc
     6601 gggtgtatgg ctcgccttaa caggaacaac agcgatctgc atgacttgct ggttgattat
     6661 tacgtgttgg gggagacgtt catggcgctg gcacggaaac atgggtgctc tgacacctgt
     6721 ataggtaaac gccttcacaa agcggagggg attgttgaag gcatgctgat gatgctggga
     6781 gtgaggcttg agatggatcg gtatgttgag cgtgaattgc cgggagggag aacctctgta
     6841 ttttatcagc gaaaaaatag tttacgatcg taaaaatctg catatcatga taagagtggt
     6901 tacattgcca cgctgcttaa cccgccgatg cgcgggtttt tttgtaccca gaatcctgtg
     6961 agctatacgg aaagtacaca gaaaggaagg tgcgaccaca attaataaca aaatcttaaa
     7021 aattgcacat ggcactatta gttttctaaa tattgtgtat tttttgtatt gcaggatgac
     7081 cctgtaacga agtttgcgta acagcatttt gctctacgag tttgccagcc tcccccagtg
     7141 gctggctttt ttatgtccgt aacatcctgt gtatcaataa atgttgttat ctacgtacgt
     7201 caagtagtcg catgagatct gaccagatat gttaaggttg cagctctctt tgaatatgat
     7261 tatcattttc attacgttat tgttacgttt atccggtgcg ccgtaaaacg ccgtccttca
     7321 gggcgtggag gatgtcaaga atatagttat cgtatggtgc tcaaggagta ttgtgtaata
     7381 tgaaaataat tatttttaga gtgctaactt ttttctttgt tatcttttca gttaatgtgg
     7441 tggcgaagga atttacctta gacttctcga ctgcaaagac gtatgtagat tcgctgaatg
     7501 tcattcgctc tgcaataggt actccattac agactatttc atcaggaggt acgtctttac
     7561 tgatgattga tagtggcaca ggggataatt tgtttgcagt tgatgtcaga gggatagatc
     7621 cagaggaagg gcggtttaat aatctacggc ttattgttga acgaaataat ttatatgtga
     7681 caggatttgt taacaggaca aataatgttt tttatcgctt tgctgatttt tcacatgtta
     7741 cctttccagg tacaacagcg gttacattgt ctggtgacag tagctatacc acgttacagc
     7801 gtgttgcagg gatcagtcgt acggggatgc agataaatcg ccattcgttg actacttctt
     7861 atctggattt aatgtcgcat agtggaacct cactgacgca gtctgtggca agagcgatgt
     7921 tacggtttgt tactgtgaca gctgaagctt tacgttttcg gcaaatacag aggggatttc
     7981 gtacaacact ggatgatctc agtgggcgtt cttatgtaat gactgctgaa gatgttgatc
     8041 ttacattgaa ctggggaagg ttgagtagcg tcctgcctga ctatcatgga caagactctg
     8101 ttcgtgtagg aagaatttct tttggaagca ttaatgcaat tctgggaagc gtggcattaa
     8161 tactgaattg tcatcatcat gcatcgcgag ttgccagaat ggcatctgat gagtttcctt
     8221 ctatgtgtcc ggcagatgga agagtccgtg ggattacgca caataaaata ttgtgggatt
     8281 catccactct gggggcaatt ctgatgcgca gaactattag cagttgaggg ggtaaaatga
     8341 aaaaaacatt attaatagct gcatcgcttt catttttttc agcaagtgcg ctggcgacgc
     8401 ctgattgtgt aactggaaag gtggagtata caaaatataa tgatgacgat acctttacag
     8461 ttaaagtggg tgataaagaa ttatttacca acagatggaa tcttcagtct cttcttctca
     8521 gtgcgcaaat tacggggatg actgtaacca ttaaaactaa tgcctgtcat aatggagggg
     8581 gattcagcga agttattttt cgttgactca gaatagctca gtgaaaatag caggcggaga
     8641 ttcataaatg ttaaatacat ctcaattcag tcagttgttg ccggtctgat aatagatgtg
     8701 ttagaaaatt tctgcatggt gaatccccct gtgcggaggg gcgactggtg aacggtatga
     8761 tctctttgat gatcgtaagc gagaatacgc gggtttggtg gcaccaggcc gaactcaccg
     8821 ggaggcaccc ggcatcatgc tgtatacaga gattaggcat atatccaggc tcctcatcgc
     8881 aggagccttt ttacatgcaa aaaaaagccc gagtgggttc gggcaacagc atgagatact
     8941 tgcattgtca tttttatcgt gcggatttta accaggattc ataaggctgc gcaactgcgc
     9001 ggcctttttc gtatttcggg ctgtagtccc cgtgtgtcat tcaggcttcc ggactacagc
     9061 ccactccata tctgatttaa tacactatcc cggccgggag gaataatgac atttaaacat
     9121 tatgatgttg tcagggcggc gtcgccgtca gaccttgcgg aaaagctgac acacaaactg
     9181 aaagagggct ggcagccgtt tggtagtccg gtggccataa ccccttatac cctgatgcag
     9241 gccattgcgg cggaaggtga tgtcaccaca ccagtggcgg tgaccggtaa tgagggtaag
     9301 gcggtggctg tcagtgccac cagcgacccg gagtattact ttgttgtggt tctggcaggg
     9361 cagtcaaatg gcatgtcgta tggtgaaggt cttccgctgc cggagacata tgaccgtccg
     9421 gacccgcgta ttaagcagct ggcgcgccgc agtacggtga caccgggcgg tgcagcatgc
     9481 aaatataacg acatcattcc ggcggaccat tgtctgcatg atgtgcagga catgagccgt
     9541 cttaaccatc cgaaagcgga cctgtcaaaa gggcagtacg gtaccgtggg gcaggggctg
     9601 catatcgcca aaaaa
//
LOCUS       DAZFXT010000164         9613 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.164, whole genome shotgun sequence.
ACCESSION   DAZFXT010000164 DAZFXT010000000
VERSION     DAZFXT010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9613
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.164"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            34..918
                     /gene="xapR"
                     /locus_tag="ACHKW0_004353"
     CDS             34..918
                     /gene="xapR"
                     /locus_tag="ACHKW0_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HFO7477072.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLRIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
     gene            complement(1027..1102)
                     /locus_tag="ACHKW0_004354"
     tRNA            complement(1027..1102)
                     /locus_tag="ACHKW0_004354"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1067..1069),aa:Lys,seq:ttt)
     gene            complement(1107..1182)
                     /locus_tag="ACHKW0_004355"
     tRNA            complement(1107..1182)
                     /locus_tag="ACHKW0_004355"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1147..1149),aa:Val,seq:tac)
     gene            complement(1229..1304)
                     /locus_tag="ACHKW0_004356"
     tRNA            complement(1229..1304)
                     /locus_tag="ACHKW0_004356"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1269..1271),aa:Val,seq:tac)
     gene            complement(1349..1424)
                     /locus_tag="ACHKW0_004357"
     tRNA            complement(1349..1424)
                     /locus_tag="ACHKW0_004357"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1389..1391),aa:Val,seq:tac)
     gene            1683..3098
                     /gene="gltX"
                     /locus_tag="ACHKW0_004358"
     CDS             1683..3098
                     /gene="gltX"
                     /locus_tag="ACHKW0_004358"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HFO7477073.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            complement(3150..3542)
                     /locus_tag="ACHKW0_004359"
     CDS             complement(3150..3542)
                     /locus_tag="ACHKW0_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7477074.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(3544..3903)
                     /locus_tag="ACHKW0_004360"
     CDS             complement(3544..3903)
                     /locus_tag="ACHKW0_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeC-like transcriptional regulator"
                     /protein_id="HFO7477075.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            4124..4199
                     /locus_tag="ACHKW0_004361"
     tRNA            4124..4199
                     /locus_tag="ACHKW0_004361"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4157..4159,aa:Ala,seq:ggc)
     gene            4239..4314
                     /locus_tag="ACHKW0_004362"
     tRNA            4239..4314
                     /locus_tag="ACHKW0_004362"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4272..4274,aa:Ala,seq:ggc)
     gene            4522..6711
                     /locus_tag="ACHKW0_004363"
     CDS             4522..6711
                     /locus_tag="ACHKW0_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HFO7477076.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMINILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(6761..7963)
                     /gene="nupC"
                     /locus_tag="ACHKW0_004364"
     CDS             complement(6761..7963)
                     /gene="nupC"
                     /locus_tag="ACHKW0_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HFO7477077.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            8299..9537
                     /locus_tag="ACHKW0_004365"
     CDS             8299..9537
                     /locus_tag="ACHKW0_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HFO7477078.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
BASE COUNT         2313 a         2251 c         2512 g         2537 t
ORIGIN      
        1 atacgatgta tttattatat ggagcactta attatggaac gcgtatacag aacagatctt
       61 aagttgctcc gttattttct tgccgtagcg gaagagttgc attttggccg cgcagcagcg
      121 cgtttaaata tgtctcagcc tccgctcagg attcatatta aagagctgga aaatcaactc
      181 ggcacgcagc tttttattcg ccattcgcgc agcgtcgtac tgacacacgc gggcaaaatc
      241 ttgatggaag aatcgcgtcg attgctggtg aatgctaata atgtattggc tcgggttgaa
      301 caaataggtc ggggagaagc agggcggatt gaactcggcg tagtgggaac ggctatgtgg
      361 ggacggatgc gcccggttat gcggcgattc ctcagggaaa atcctaacgt tgaagttctt
      421 tttcgcgaaa agatgcccgc gatgcaaatg gccctgctgg aacgacgtga actcgatgcc
      481 gggatctggc gaatggcgac agaaccaccg acaggtttta ccagcttacg gttgcatgaa
      541 tcggcgtttc tggtggcgat gcctgaagag catcatctct catcattttc caccgttccg
      601 ctggaagcgc tacgtgacga gtattttgtt acaatgccgc ccgtgtacac tgactgggat
      661 tttttgcagc gagtttgcca gcaggtggga ttttcaccgg ttgttattcg cgaagttaat
      721 gaaccgcaaa cggtactcgc catggtcagt atgggtattg gtatcacatt gatagcggac
      781 agctacgcac aaatgaactg gccaggtgtc attttccgtc cgctcaaaca gcgcatccct
      841 gcagatttat atattgttta tgaaacacag caggtgacgc ccgcgatggt taagctgttg
      901 gctgcattga cacagtaggt gaggagacag gaattgccgg aaatagtcgt ctgctaagtg
      961 aagcgcatca ggcaatttcg caaatcgcag atagcaaaaa agcgccttta gggcgctttt
     1021 ttacattggt gggtcgtgca ggattcgaac ctgcgaccaa ttgattaaaa gtcaactgct
     1081 ctaccaactg agctaacgac ccgaagtggt gggtgatgac gggatcgaac cgccgacccc
     1141 ctccttgtaa gggaggtgct ctcccagctg agctaatcac ccacttcggt acttcacatt
     1201 tttacaagaa atttagctgg cgagaaagtg gtgggtgatg acgggatcga accgccgacc
     1261 ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccacttct caatttcttg
     1321 ctacacggcg gagactacat aaagtagttg gtgggtgatg acgggatcga accgccgacc
     1381 ccctccttgt aagggaggtg ctctcccagc tgagctaatc acccccgctg tgtggagtcg
     1441 cattataggg agagttcaaa atgagtcaac gcattttcta aagaaattgt tcgttcgtcg
     1501 taaatttaag caagatgatc gcaaaacaga ccgtgttgcg caatttgtca acgaaaacaa
     1561 taatgcgtaa ggtagaaacc cgaactacat tgaggaatca ggcgggagtg atagaatatc
     1621 gcccactcaa tttttccagg atttgccggt tgtcggcatc tttctaaacg taaggccatt
     1681 tcatgaaaat caaaactcgc ttcgcgccaa gcccaacagg ctatctgcac gttggcggcg
     1741 cgcgtactgc tctttactcc tggctttttg cacgtaacca cggcggtgag ttcgtgctgc
     1801 gtattgaaga caccgatctt gagcgttcca cgccggaagc tatcgaagcc attatggatg
     1861 gcatgaactg gctgagcctg gagtgggatg aaggtccgta ctaccagacc aaacgttttg
     1921 atcgctacaa tgcggtgatc gatcagatgc tggaagaggg cactgcttat aaatgctatt
     1981 gctctaaaga gcgcctggaa gcgctgcgcg aagagcaaat ggcgaaaggt gagaagccgc
     2041 gttatgacgg tcgctgccgc cacagccatg agcatcatgc tgatgatgaa ccgtgtgtcg
     2101 tgcgttttgc taatccgcag gaaggttctg ttgtttttga cgatcagatc cgtggtccga
     2161 tcgagttcag caaccaggag ctggatgatc tgattatccg ccgtaccgat ggttccccaa
     2221 cctataactt ctgtgtggtt gtcgatgact gggatatgga aatcacccac gttatccgtg
     2281 gcgaagacca tatcaacaac acgccgcgcc agatcaacat tcttaaggcc ctgaaagcgc
     2341 cggtgccggt ttacgcgcac gtttctatga tcaatggcga tgacggtaaa aaactgtcca
     2401 aacgtcacgg ggcagtcagc gtaatgcagt atcgtgatga cggttatttg ccagaagcac
     2461 tgctgaacta tctggtgcgt ctgggctggt cccacggcga tcaggaaatc ttcactcgtg
     2521 aagagatgat caaatacttc actttgaatg ccgtcagcaa atctgccagt gcgttcaaca
     2581 ccgacaagct gctgtggctg aaccatcact acattaacgc gctgccgccg gagtatgttg
     2641 ctactcactt acagtggcac attgagcagg aaaatatcga tacccgtaac ggcccgcagc
     2701 tggctgatct ggtgaaactg ctgggcgaac gctgcaagac gctgaaagag atggcacaga
     2761 gctgccgtta tttctacgaa gattttgctg agttcgatgc cgatgcggcg aaaaaacatc
     2821 tgcgtccggt agcgcgtcag ccgctggaag tggttcgtga caaactggcc gcgattactg
     2881 actggaccgc tgaaaacgtt catcacgcta ttcaggcgac ggcggatgag ctggaagtgg
     2941 gtatgggtaa agttggtatg ccgctgcgtg tcgccgtaac cggtgcgggg cagtctccag
     3001 cactggatgt taccgttcac gcaattggta agacccgcag tatcgagcgt atcaacaaag
     3061 cgctggattt tattgctgaa cgcgaaaatc agcagtaatt agcgtgtaaa aagataaacg
     3121 gcaggagata atatcctgcc gttttttatt tatgctgatt cgtcaatatc gagacgggcg
     3181 aggaaattgc gaattccttc acgtgacaaa aattgcgcga ctttttcctg ctcaatgtct
     3241 gtcatattat ctatcgcgtt gatgatttgt cgatacgccg gggcgcgtgc tccaggagcg
     3301 tattcagcaa ttctctcttc agcttccatc aacattggtg tgttatggaa ggctggcgtg
     3361 ttctgaataa actcgcaaac ttgcgtatcg accagaataa gtctggcacg accgcctttc
     3421 acgccgggaa atttttccgt tttccagcct ttttctctga tccaacggtt aacagtttgt
     3481 ttagccacac cgaggcattc agccagttct tcggtggtca ttttgttgcg taatcttttc
     3541 atattatttg ctatcgcgaa tccctaagcg ttgtaacaat ccgataatcc cttcccgcag
     3601 aagcaaggat gtaaactgtt tttgttcaac tggcgtcatc tctttcgcca gtgtcaccag
     3661 caatacttca agcggcgcat caccagaaag ggcaggcgct tctccctgac cttctggacg
     3721 ttcagcattg cgaatatatt cacgaacctg ttcattgacg tgaaccagtc gggctttgcc
     3781 accctggacg ccaggttttg gtgacgttgt ccagccttcc ttgcgtaccc atttattaat
     3841 ggtctggcgg ctatagccgg tcagtctggc aagttcatct ggtgtcatcc tctccttgaa
     3901 cataacaatt ccctgaatgt atgtgggtct attagtagtt cattttataa caccacttta
     3961 caggaaaccg tgacatgaat aactactgaa tgcgagcggt tttccaaaat tgttcatttg
     4021 cctgattttt cagcgattga aattacacgt tgcaatttgc cgttgacaca ttcgggcgga
     4081 atttatatga tgccgcccgt caactcgaca agctttacgt gacggggcta tagctcagct
     4141 gggagagcgc ttgcatggca tgcaagaggt cagcggttcg atcccgctta gctccaccaa
     4201 attttgcacc cagcaaattt ggtacgtaaa cgcatcgtgg ggctatagct cagctgggag
     4261 agcgcttgca tggcatgcaa gaggtcagcg gttcgatccc gcttagctcc accaaatttc
     4321 caaccctcgc tgcaaagcgg gggttttttg tctctgcttt ttgccgcttt tgtcatacag
     4381 tctacgtccg ggttagcgcc gcctggtgaa agcatcattg gatgaaaaat cggcaacagg
     4441 ctggcccctg tttgcttcgc gatgcgaata aacttattat ttgtgtgcct gaaaaccccg
     4501 atcagtgaga gtagtgtact catgtttgtg gagcataacc tgataaaaaa tatcaagata
     4561 ttcacactag cgtttacgct caccgtggta cttattcagc tatcccgttt tatttcgcca
     4621 cttgccatta tccattccag ttatatcttt ctggcgtgga tgccactgtg cgtaatgctg
     4681 tcaatcttgt ttatctttgg ctggcgcggt gtcgttcccg ttttatgcgg gatgttttgc
     4741 accaatctgt ggaactttca tctctctttt ttacagactg cggtcatgct cggtagccag
     4801 acgtttgtcg tgttgtgtgc ctgcgcaata ttacgctggc agctggggac gcgttggcgt
     4861 tatggattga ccagccgata tgtctggcaa cgtctgttct ggcttggttt ggtgacgccg
     4921 atcggcatca aatgcagcat gtatcttgtg gggagcttct ttgatttccc gctaaagata
     4981 tccacctttt tcggcgatgc ggatgccatt ttcacggtcg ttgatttgct aagccttttc
     5041 accgctgtgc tgatttacaa catgcttttc tactatctca ctcgcatgat tgtaagtccc
     5101 cactttgcgc agatattgtg gcgcagggat atcgcgccgt cgttgggcaa agagaaacgc
     5161 gcatttacct taagctggct ggcagcgtta agcgtgcttc tacttctgtt gtgcacacct
     5221 tatgaaaacg actttattgc cggttacctg gtacccgttt tcttcatcat ctttaccctc
     5281 ggggtcggta agcttcgcta tccgttttta aatctcacct gggctgtttc aacgctttgc
     5341 cttctgaatt acaaccagaa ctttttgcaa ggggtggaaa ccgaatattc gctggcattt
     5401 attcttgcgg tgctgatttc ctttagcgtt tgcctgctct atatggtgcg catttatcat
     5461 cgcagtgaat ggcttaatcg ccgctggcat ttgcaggcgc tgacagatcc gttaacgctc
     5521 ctacccaact ttcgtgcgtt ggaacaagcg ccggaacaag aggcgggcaa gagtttttgc
     5581 tgcctgcgca ttgataatct tgagtttatg agtcgtcatt acggcttaat gatgcgcgtt
     5641 cactgtatcc gctcaatttg ccgtacgctg ctgccgttga tgcaggaaaa cgaaaagttg
     5701 tatcaattgc cgggtagtga actgctgtta gtgctgagcg ggccggaaac ggaagggcga
     5761 ctccagcata tgattaacat cctgaatagt cggcaaattc actggaacaa taccgggctg
     5821 gatatgggct atggtgctgc ctgggggcgt tttgatggaa atcaggaaac cctgcaaccc
     5881 ttgttggggc agttaagctg gctggcggag caatcctgcg cacatcatca tgtgctggcg
     5941 ctggatagca gagaggagat ggtttccggg cagaccacta aacaggtgct attgctgaat
     6001 accattcgca cggcgttaga tcagggtgat ttgctgctct acgcccagcc aattcgcaac
     6061 aaagagggtg aaggttatga tgagatcctc gcgcgactga aatatgacgg cggcattatg
     6121 accccggata agtttctgcc ccttattgct cagtttaacc ttagcgcgcg ttttgatttg
     6181 caagtgctgg aatccttgtt gaagtggctg gcaacacacc cttgcgacaa aaaaggaccg
     6241 cgcttttcag tcaatttaat gccgctcacg ctgctgcaaa agaatattgc cgggcggatt
     6301 attcgtctgt ttaagcgtta tcacatctcc ccgcaggcgg tcattcttga gatcaccgag
     6361 gagcaggcgt tttctaacgc agaaagcagc atgtacaaca tcgagcagct gcataagttt
     6421 ggtttccgga ttgcgattga tgactttggc accggatatg ccaactacga acggttaaag
     6481 cgtttgcagg ctgatatcat caaaattgat ggcgtctttg tgaaagatat tgtcacgaac
     6541 acgctggatg cgatgattgt gagatcaatt accgatctgg cgaaagcgaa gtcattgagt
     6601 gtggtcgcgg agtttgtcga gacgcaacag cagcaggcgc tattgcataa gctcggggtg
     6661 caatatctgc aagggtattt gattggtcgc ccgcagccat tagctgatta acaggcgtaa
     6721 aaaaaccggg gaattatccc cggctttttt atgtatggtc ttacagcacc agtgctgcga
     6781 ttgacgcaga cagcacactc accagggtag agccgtaaac cagcttcaga ccgaagcgag
     6841 aaaccacgtt accttgctct tcattcaggc ctttaactgc acctgcgata atcccgattg
     6901 aagagaagtt agcgaaggaa accaggaaca cagagatgat gccttcagca cgcggagaga
     6961 gcgtggaagc aattttctgc agatccatca tcgcaacgaa ctcgttggaa accagtttgg
     7021 tcgccatgat actgcccact tgcagtgctt cactggaagg aacacccatc acccatgcaa
     7081 tcggatagaa gatgtagccc aggatgccct ggaaggagat gctgtagcca aaccagccag
     7141 taacggtggc aaacagtgcg ttcagcgcgg cgatcagggc gataaagccg atcagcatcg
     7201 cggcaacgat aatggcaact ttgaaacctg ccagaatgta ttcacccagc atttcgaaga
     7261 agctctgacc ttcgtgcagg ttggacatct ggatgttttc ttcactggca tcaacacggt
     7321 aaggattgat cagcgacagc acgataaagg tgctgaacat gttcagtacc agcgcagcaa
     7381 cgacgtattt cggttccagc atggtcatgt atgcaccaac gatggacatc gacacggtgg
     7441 acattgccgt ggcagccatg gtgtacatac gattacggga gattttgccg aggatatctt
     7501 tataggcaat aaagttttca gactgaccca gaatcaggga gctgacggcg ttaaaggatt
     7561 ccagtttgcc cataccgttg actttggaga gcaggaaacc aattgcgcgg ataataaccg
     7621 gcaacacgcg aatgtgctgg agaataccga tcagtgcaga gataaagacg attgggcaca
     7681 gcactttcag gaagaagaat gccaggcctt gatcattcat gctaccaaag acgaagttag
     7741 tcccttcgtt ggcaaatccg agcagttttt cgaacatttc ggagaagcct ttcacgaagc
     7801 ctaaaccaac gtcggagttc aggaagaacc acgccagtaa cacttcgata acaagcagtt
     7861 gaataacata acggatacga atttttttgc ggtcgctgct taccagcagt gcgagaatcg
     7921 caacaacggc aagtgccagt acaaaatgaa ggacgcggtc catatttgct ccaaatatga
     7981 ggcaggttaa atttccgtgc acattctatg taacatggtt aaggaaaacg agatctgaca
     8041 cacactataa tgacgtcctg tgacaagctt caaaaatagt gatctgtcat acatttctgc
     8101 tatgttgtgt atggaagctg aaagttatgt aaatgtgcta acattaaaca taaccataac
     8161 attcaaatca tcaatctttt cgtagtcata atcctggtct atcaaagaaa tcaccacaac
     8221 ccattcaaat gaatttgata atcattctcg tttggcatag catgaaacat agcaaaggct
     8281 atgttttaga ggcacaagat gacgaactat cgcgttgaga gtagcagcgg acgggcggcg
     8341 cgcaagatga ggctcgcatt aatgggacct gcgttcattg cggcgattgg ttatatcgat
     8401 cccggtaact ttgcgaccaa tattcaggcg ggtgctagct tcggctatca gctactgtgg
     8461 gttgtcgttt gggccaacct gatggcgatg ctgattcaga tcctctctgc caaactaggg
     8521 attgccaccg gtaaaaatct ggcggagcag attcgtgatc actatccgcg tcccgtagtg
     8581 tggttctatt gggttcaggc agaaattatt gcgatggcaa ccgacctggc ggaatttatt
     8641 ggtgcggcga tcggttttaa actcattctt ggtgtttcgt tgttgcaggg cgcggtgctg
     8701 acggggatcg cgactttcct gattttaatg ctgcaacgtc gcgggcaaaa accgctggag
     8761 aaagtgattg gcgggttact gttgtttgtt gccgcggctt acattgtcga gttgattttc
     8821 tcccagccta acctggcgca gttgggtaaa ggaatggtga tcccgagttt acctacttcg
     8881 gaggcggtct tcctggcagc aggcgtgtta ggggcgacga ttatgccgca tgtgatttat
     8941 ttgcactctt cgctcactca gcatttacat ggcggttcgc gtcaacaacg ttattccgcc
     9001 accaaatggg atgtggctat cgccatgacg attgccggtt ttgtcaatct ggcgatgatg
     9061 gctacagctg cggcggcgtt ccacttttct ggtcatactg gtgttgccga tcttgatgag
     9121 gcttatctga cgctgcaacc gctgttaagc catgctgcgg caacggtctt tggattaagt
     9181 ctggttgctg ccggactgtc ctcaacggtg gtggggacac tggcggggca ggtggtgatg
     9241 cagggattca ttcgcttcca tatcccgctg tgggtgcgtc gtacagtcac catgttgccg
     9301 tcatttattg tcattctgat gggattagat ccgacacgga ttctggttat gagtcaggtg
     9361 ctgttaagtt ttggtatcgc cctggcgctg gttccactgc tgattttcac cagtgacagc
     9421 aagttgatgg gcgatctggt gaacagcaaa cgcgtaaaac agacaggctg ggtgattgta
     9481 gtgctggtcg tggcgctgaa tatctggttg ttggtgggga cggcgctggg attgtagttg
     9541 aatgagcgtc gcatctggca ctattggcgg atgcggcgta aacgccttat ccgccctacg
     9601 cgttaaagag ccg
//
LOCUS       DAZFXT010000165         9595 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.165, whole genome shotgun sequence.
ACCESSION   DAZFXT010000165 DAZFXT010000000
VERSION     DAZFXT010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.165"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHKW0_004366"
     CDS             complement(65..823)
                     /gene="yhdZ"
                     /locus_tag="ACHKW0_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HFO7477079.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFTHPKSERTRAFLSQVIH"
     gene            complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHKW0_004367"
     CDS             complement(831..1934)
                     /gene="yhdY"
                     /locus_tag="ACHKW0_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7477080.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1944..3125)
                     /gene="yhdX"
                     /locus_tag="ACHKW0_004368"
     CDS             complement(1944..3125)
                     /gene="yhdX"
                     /locus_tag="ACHKW0_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HFO7477081.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3193..4218)
                     /locus_tag="ACHKW0_004369"
     CDS             complement(3193..4218)
                     /locus_tag="ACHKW0_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7477082.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(4649..4870)
                     /locus_tag="ACHKW0_004370"
     CDS             complement(4649..4870)
                     /locus_tag="ACHKW0_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477083.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5123..8227)
                     /gene="acrF"
                     /locus_tag="ACHKW0_004371"
     CDS             complement(5123..8227)
                     /gene="acrF"
                     /locus_tag="ACHKW0_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HFO7477084.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPVSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNGDENSAEAVIHRAKMEFGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8239..9396)
                     /gene="acrE"
                     /locus_tag="ACHKW0_004372"
     CDS             complement(8239..9396)
                     /gene="acrE"
                     /locus_tag="ACHKW0_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HFO7477085.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
BASE COUNT         2550 a         2482 c         2290 g         2273 t
ORIGIN      
        1 catcaaatca agcaaaaagg ccatccgaaa ggatggcctt tttgcttttc gaactaacat
       61 tcaattaatg gattacctgc gataaaaatg ccctcgtacg ctctgattta ggatgcgtaa
      121 aaaattcatc aggtgcagct tgctccacta tttccccacg atccataaaa atcacccggt
      181 cagcgacggt tcgcgcaaac cccatctcat gcgttacaca caacattgtc atacccgatt
      241 gcgccagccc aatcatcgta tccagcacct ctttcaccat ctccggatcg agcgccgacg
      301 ttggctcatc aaacaacata attttcggct tcatacacag cgaacgcgca atggcaacgc
      361 gttgctgctg accaccagaa atttgtccgg gaaacttatg cgcatgttcg gcaattctca
      421 cccgctcaag gtaatgcatc gccagagctt cagcctcttt ctttggcatc ttgcgtaccc
      481 aaatcggtgc cagggtacag ttctgtagaa cggtcagatg agggaagaga ttgaaatgct
      541 gaaagaccat tcccacttcc tgccggacgc gctcaatatt gcggatatct tcattaagtt
      601 cgatgccatc taccacgatc cgtccctgtt gatgttcttc cagatgatta atacaacgaa
      661 tggttgtcga tttaccggaa cctgaagggc cacacagaac gatccgttct cccggttgca
      721 cggttaaatt tatatttttc aaaacatgga attgtccata ccatttattg acgttttcca
      781 gcgtaatcat cgcgttagca ggttgcagta aaatttggct catagtgtcc tcaatgcggt
      841 gtacgcccgg tgttaaaacg tttttccaga tgctggctat agcgcgacat gctgaaacag
      901 aagatccagt agatcagcgc ggcgaaaaca tacccttccg tcgacatacc caaccaggtg
      961 ggatcaacgg ttgcctgctg aacgctactg aaaagatcga acaacccgat aatgatcacc
     1021 aggctggtat ctttgaagag tgcgatgatg gtatttacca gcccaggaat taccagcttc
     1081 aacgcctgtg gcagaataac cagcccctga gttttccagt aacccaacgc cagcgactct
     1141 gccgcttcat attgcccttt aggcagcgcc tgtaatccgc ctcgcacgac ttccgcaaca
     1201 tatgctgact gaaacaggat cacgccaacc agcgcgcgga tcagtttgtc gatattggtg
     1261 ccttctgcca taaacaaagg cagcatgacc gaagacataa acaatacggt aatcagcggt
     1321 acgccgcgcc agaactcgat aaaaatgacc gataagatac gcacaatcgg catatgggag
     1381 cggcgaccta acgccagtaa gatcccccac ggtagcgccc ccgcaatccc aacggatgca
     1441 ataattaaag ttagcgtcag cccgccccat tgccgggttt caacccgctc cagaccaaaa
     1501 aaaccgccat acatcagcca ccagacaatc agtgggtaaa tcaccgccca gaccgcaata
     1561 tagcgaccgc gatgcgggag tattttccag aacattggtg cgattgacac aaggccaatc
     1621 agtaatgcca ggttaattcg ccagcgttgg tcgtgtgggt aaagtccata cataaactga
     1681 ccaaatcgtt cgtggatgaa gacccaacag gcaccggctt ttgtgcagtc ggcacgcgtt
     1741 gagccaaccc aattagcctg caaaaatgcc cagttcagca acggtgggat cagttcccac
     1801 atcaaccata tgcagccaat agtcagcagg ctattgctcc agctggagaa cagatttttt
     1861 cgcacccaca ccatcgcgcg gcttgagtta tggctcgctg ggcgcgaggg ctgagacagc
     1921 aatacttttg tcatcatgac tccttagcgt tcaacgatcg cgatgcggcg gttatagata
     1981 ttcatcagca gcgagatagt caggctgata atcagataga ccgacatggt catggctatc
     2041 gtctcgatgg cttgccccgt ctggttcagc acggtgccag caaacagcga aaccatatcg
     2101 ggatagccaa tagcggcggc aagagaggag tttttgacga tgttgagata ctggctggtt
     2161 aacggcggaa taatcactcg cagtgcctgg ggaataatga cctggcgtag cgtaaccggg
     2221 ttgggtaatc ccagtgaccg agccgcttca tgttgaccat aaggcactgc ctggatccca
     2281 gcgcggataa tctcggcgat aaatgcagat gtataaaccg aaagtgccag cgttaaggct
     2341 gccagttcag gaattaaaac catcccgccg cggaagttaa agcctcgtag ggctgggaca
     2401 tcccagtgta gtgctgcgcc aaaaagccat tgcgccagta aaggcaaacc aatgatcaac
     2461 actgcggcga tcggccaggt gcggcgcagt tgtccggtct ttatctggta tgttttatta
     2521 aagcggaata gcccaacaga aaggactata gccataacaa cagccaggat aaacgcaata
     2581 aatccgtctc ccagctgcgg tgacggaata taaagcccac gattgctcaa aaaggccaga
     2641 tcaaacgcgc tcacagcctg gcgtggtccg ggcaaattgc gcaacacggc aaagtaccag
     2701 aagaagattt gcagcagcgg gggaatatta cggaagatct caatataaat tgtggatagt
     2761 tttcgtagca gccagttatc cgaaagtctc gccagaccga taaagaagcc taaaacagaa
     2821 gcgaacacaa tacacaatgc agaaaccagt agcgtattga gtaagccgac aataaaaacg
     2881 cgtccgtagg tgtcgccctg ctggtaatcg atcaaatgct ggacaatacc gaagccagcg
     2941 ccgcgatcca gaaaggcaaa acctgaagta atgccacgat tattgagatt cgttacagtg
     3001 ttgtgaaata accaaccaac aatgccgaca acagcaacaa cggcaaggat ctggaataac
     3061 caggcgcgaa ccgtagggtt ggcaaaagag agtgagcctt taacggttga gcggcgatga
     3121 gacatagaaa cctcggaaag cataacgctg aagagagcgc cgccgtagcg acgcccgtta
     3181 cctcacaccg acttaacgca cgggcggcgc gtactgaata ccgccgttat tccagagatt
     3241 attttgccca cgtttaattt tcagcgggct ttctgaacct acgttacgct cgaaaatttc
     3301 cgagtagtta cccacctgtt tgatgatgtt ataggcccat ttattatcca gcttcagatc
     3361 cttgccgtaa tcgccttctt tacccagcag atgtgccata tcaggcgttg ctggattagc
     3421 cgctttttca tcgacgttct gggaattgat gcccatctct tcagcattca gcatggcgaa
     3481 aagcgtccag cgtacaatcg agaaccattc atcatcgcca cgacgaacta ccggcccaag
     3541 cggttcttta gagataacct ccggtaagac aatccattca gctgggttgc ttaatttgat
     3601 gcgcagggca tacagttgcg actgatccga ggccagcgta tcgcagcgac cagattccag
     3661 tgccttcgct gattcgtcag agcgatcgaa agtcaccggt gtgtacttca tattgtttgc
     3721 cttgaaatag tcggcaacgt tgagttcggt atcagtaccc gcctgaatac agacggtagc
     3781 gccatccagt tctttcgcgc tttttagccc cgctttatcg tgcgtcagga agccaatgcc
     3841 gtcgtaataa gtgacgccag taaatgccat tcccatcccg gcgtcgcgag atgaagtcca
     3901 ggtcgtatta cgggagagca aatccacttc ccctgactgt aaagccgtga agcgttcttt
     3961 tgctgtgagt ggggtatatt tcactttcgt gtcgtcacca aatacagcag cggcaacacc
     4021 acgacaaaca tcaacatcaa tacctgaaaa cttaccgtct gcatcggcat aagagaaccc
     4081 aggtaatcca tcactgatcc cgcattgcac aaaacctttt ttctgaacgg catcaagcgt
     4141 cgcgccagca tgcgcctgat ttgcaacggc aagcagcacg ctggcggcag ccagtgtggc
     4201 tatcatcatc tttttcataa tgcttcctgt gaggcgaaaa ttatcgttat aggacgtttg
     4261 ggaaacgtct tttacctgtc tgtggccatc ctcttataag caaaagtagt gccagctttt
     4321 gcgcccgatt tcgcgtcgca ataagctgag tgtagacagg aaataataaa aacagcgccc
     4381 cgcaatggtg cgaaaatgaa acatgcgcca cattttggcg caaaatattt acgcgtgttt
     4441 tactaagcag aataatgatt gttagcgtga gtatatttct ggtgggaagt gtgaaagtta
     4501 aattgatgcg ttatttcgaa agcagcgaaa taacgcgata taacatgcta aatcagcaga
     4561 gaattagcca gcagaatata gcaatatttt atcttatgta aagatactgt atcgttaaac
     4621 agagcgcgaa actccgcgcc ctgttcagtt atttcgacag agcgatgatc cccatcgtca
     4681 ggccagcaac agccgctgcc ccgccggcaa tagccccagc cgtttcccag ttatcctgag
     4741 tcgtgccttt catacaggta ttggtgtgaa ccagacgacc ctgatcgtca taaaccggaa
     4801 cgcagggcga atcgtgagca cagcccgcca gcaatgcggt gagcaatgcc acaggaatta
     4861 atcttttcat ggtgttacct cagtaataaa cgctaacgct taataagtta actctcttat
     4921 taacgagctg aatgccattt tatcaccgga ggttataaat attgcggaat gaataatgac
     4981 aaattatgtg aattgtaaaa atagtgaagg gagcgggaac tatagaaagt ttttaaagag
     5041 tgagtggaag aatatcttat taatttttat gggtttcact gaaaataata aaggcacccg
     5101 aaagtgcctt tatgtttctg atttatcctt taaagcaacg gcggatcacc acaaagaaca
     5161 ccggtacgaa gaagattgcc agcaacgttg cagagaccat tccgcccatt accccgatac
     5221 ccactgcgtt ctgcgcgcca ctgccggcac cgttactgat agctagcggt aatacgccga
     5281 gaataaaggc gagagaggtc atcaggatag gacgcagacg catacgtact gccattagtg
     5341 tcgcttcaac aacaccttta ccctctttct ccatgagatc tttagcgaac tcaacaatca
     5401 aaatagcgtt tttggccgac aagccaattg tcgttagcaa gcccaccata aagtagacgt
     5461 catttttttg attaaagagt gtcgccgcca gcagcacgcc gacaatccct aacggcacga
     5521 ccaacataac cgagacagga attgaccagc tttcatagag tgcagcaagg cacaggaaaa
     5581 caaccacaaa ggaaattgct accagagcgg gagcctggtt tcccgataag cgttcctgat
     5641 acgacatacc cgtccagtca taaccaatgc cagcaggtaa ttttgacgca aggttttcca
     5701 tcaacgccat ggcatcgccg gaactggttc ctggcgcggc ttccccctga atctccattg
     5761 acggcagacc gttgtagcgt tccagtcgcg gagagccata cacccaatgt gaagtggtaa
     5821 aggccgagaa tggcaccatt tcgccgttgg cgctgcggac ataaagttta tcgacatctt
     5881 ctggcagcat acggaatttg gcatccgcct gaacatacac ctttttcacg cgaccacggt
     5941 cgatgaagtc gttaacgtaa gtcccaccca acgccgttga aatggtctga ttgatgtcag
     6001 aaagtgaaac acctaatgcc tgcgcctttt cctggtcaac ttccagttta aactgcgcgg
     6061 tgtcttccag gccattagga cgcacgctga ctaagctggc aggatgttgc gccgccatac
     6121 caagcaactg gttacgggcc tgggttaggg catcgtgacc cagcccagcc tgatcaatta
     6181 actcaaagtc gaaacccgtt gccgtgccca gttcaacaat ggctggcata ttgaatggaa
     6241 tgacaaaacc gtcgcggatc ttgccaaatt ccattttggc acgatggatt accgcttccg
     6301 cactgttttc atcaccatta cgctcttccc acggtttcag actgacgaag gccataccgg
     6361 cattttgtgc ctggccgctg aagctaaagc cgttaaccgt aaagacactt tcaacgttcg
     6421 ctttctcgtt cttcagatag taatccgtca cttgatccaa cactttttgc gtccgctctt
     6481 gcgtcgcgcc agcgggtaac tgaatcatgg tcagaaagac accctgatcc tcttcaggta
     6541 agaaggaaga cggaagacgt aaaaacaaca ccaccattcc tgcaacaatc agcgcataga
     6601 tcagtaaata tcgtcctgtg gaaccgagga ttttgccgac gctgttggtg tagtggttaa
     6661 cgctatgatc gaaggtagta ttaaaccagc cgaagaaacc gcccttattt tcgtgatgct
     6721 cagcagagac gggtttaagc agcgttgcac ataacgcagg ggtaagaatc aatgccacca
     6781 gaacagaaag cgccattgcc gaaacgatgg tgatagagaa ctggcgataa attgccccag
     6841 tagaaccgcc gaagaatgcc atcggaataa ataccgctga cagcaccatc gcgataccca
     6901 ccagtgcgcc ctggatttgc gacattgatt tttccgtcgc ttcttttggc gggagcttat
     6961 cctccatcat cacgcgctcg acgttctcca ccaccactat cgcatcatcg acgagcagcc
     7021 cgatggcaag caccatcccg aacatcgtta gtgtgttgat ggagtaacca aaagcggcga
     7081 ggatggcaaa cgtccctaat aacaccacgg gtaccgcaat ggtggggatc agcgttgctc
     7141 gcatattctg caagaacaga tacatcacca ggaacaccag cataatggct tcaaacagcg
     7201 tttttaccac ttcgtgaata gaaagctgga cgaatggcgt ggtgtcataa gggtagagaa
     7261 ccttcattcc ctgcgggaag aatggctgta attccgccag ttttgcctta atggctttcg
     7321 cggtatcgag agcattcgcg ccggttgcca gcttaatccc caggcccgcc gccggttttc
     7381 cgttgatacg agcgataacg ttatagtttt caccgccaag ttcaactcgt gcgacatctt
     7441 tcaggcgtac caccgagccg tcactgttta cgcgcagggt tactttgccg aattcttccg
     7501 gattttttaa ccgcgtctga gcaataattg aggcgttcaa ctgttgccct ggtaacgctg
     7561 gcgttccgcc caactgtccg gcagcgatct gatcgttctg taccttcaac tggttaatca
     7621 catcaaccgg tgtcagttta tatttgttta gcagatcggc gtccagccag atacgcatcg
     7681 catactgtgc gccgaaaagc tgtacgtcac cgacgccatt cagacggcta agcgtatctt
     7741 taacgttaga ggccacatag tccgagatat cgtcctgtgt ggtgtctggg ttatcagaga
     7801 caaagcccgc caccatcaaa tagctgctac tggacttttc aacactgatc ccctgctgct
     7861 gaacctcctg cggcagcaac ggcgtggcga gctggagttt gttctgcacc tgcacttgcg
     7921 cgatatcagg atcggtcccg gactggaagg taagggtaat tgtcacgcta ccggcggaat
     7981 cgctggtgga ggacatatac atcaggttat cgataccgtt catattctgt tcgataacct
     8041 gcgtcaccgt atcctgcacg gtctgcgcat cagcgcccgg atagtttgct gaaacagaaa
     8101 ccgcaggcgg tgcaatggtt ggatactgag cgacgggtaa ttgtaggatc gccagtgcgc
     8161 ccgccatcat cagaataatg gccagcaccc atgcaaatat cggtcgtcga ataaaaaagt
     8221 ttgccatgtc agattacctt acttcgatgc agtatctgcg ggggtatcgg tagtggcttt
     8281 aacctgctct cccggacgcg ctttttgcag gccgctgaca atgacttgat cgccagattt
     8341 cagaccttcg ctaatcaacc atttatcgcc aatcgcctga ctggcaacga cagggcgcgc
     8401 ttccacctga cttttatcgt taacaatcag cacggttgca tcaccacgcg gtgtgcggct
     8461 aacgccttgt tgcgggataa gaatggcgtc aggttggacg ccttcatcaa tccgtgcgcg
     8521 cacaaacata cccggcaaaa gcgtatgttg cgggttaggg aagacggcac gtagggttat
     8581 ggagccggtg ctttcatcaa cggtcacatc agagaattgc agcgtacctt tcaggggata
     8641 ggtttgaccg ttttccatga ccaactctac gttgctggtg gcgttttcct tatgcaaatt
     8701 tccttgctct acggattgct tcagcctcat aaaatcgttg ctggattggg tcacatcaac
     8761 gtagatagga tcgagctgct ggacagtcgc cagttcagtc gtttgcccat tagtgacaag
     8821 agcgccttcg gtcacggtcg atttgccgat acgtccgcta attggcgcag tcactttggt
     8881 ataagcgaga ttgatgcgag cgctttcgac tgtggctttt gcggcaatca cggcggcatc
     8941 ggcctggcga gcatcagcaa tggcctggtc gtactcctgc tggctgatgt atttcgtacc
     9001 cacgagcgga acgtaacgtt ttaccgtcaa atgcgcgatg gcggcggcgg cttcactttt
     9061 cgccagttcg cctttcgcgc tgtcataatt tgcctgatag gtcgcgggat cgatctggta
     9121 cagggactgg cctgcttgta catcgctgcc ttcagtgaaa ttacgattca gtacgatccc
     9181 gctaacctgt gggcgaactt cggctatacg gtaagcattg gtgcggcctg gtaattcagt
     9241 cttaacttct aacggggccg ttttcacaat atgaacggta acctgcggtt caccgacgtg
     9301 agctttctct tctcccttat cgttacaacc ggcgattaaa gccgcggaga tcagaataaa
     9361 ggagggcagg aggaaaaacc tggcatgttt cgtcattact attcctcaaa aaaccaaaag
     9421 cgcgttattt acccagaaag gcaacgcgtt aactcgcaga aagaaaaata cagtttgcta
     9481 tcctacaaat tatcattcgt cgatgtaagg aatagttatg aatactggcg tctcaaggca
     9541 cataaacaca aaaaaagatt aatattctac tgttttattt tgacgcgggt tgaaa
//
LOCUS       DAZFXT010000166         9385 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.166, whole genome shotgun sequence.
ACCESSION   DAZFXT010000166 DAZFXT010000000
VERSION     DAZFXT010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9385
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.166"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(16..642)
                     /gene="upp"
                     /locus_tag="ACHKW0_004373"
     CDS             complement(16..642)
                     /gene="upp"
                     /locus_tag="ACHKW0_004373"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HFO7477086.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            967..2004
                     /gene="purM"
                     /locus_tag="ACHKW0_004374"
     CDS             967..2004
                     /gene="purM"
                     /locus_tag="ACHKW0_004374"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HFO7477087.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNSNGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            2004..2642
                     /gene="purN"
                     /locus_tag="ACHKW0_004375"
     CDS             2004..2642
                     /gene="purN"
                     /locus_tag="ACHKW0_004375"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HFO7477088.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            2813..4879
                     /gene="ppk1"
                     /locus_tag="ACHKW0_004376"
     CDS             2813..4879
                     /gene="ppk1"
                     /locus_tag="ACHKW0_004376"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HFO7477089.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            4884..6425
                     /gene="ppx"
                     /locus_tag="ACHKW0_004377"
     CDS             4884..6425
                     /gene="ppx"
                     /locus_tag="ACHKW0_004377"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HFO7477090.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(6464..8707)
                     /gene="pdeF"
                     /locus_tag="ACHKW0_004378"
     CDS             complement(6464..8707)
                     /gene="pdeF"
                     /locus_tag="ACHKW0_004378"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HFO7477091.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(8889..9041)
                     /locus_tag="ACHKW0_004379"
     CDS             complement(8889..9041)
                     /locus_tag="ACHKW0_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477092.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            9059..9250
                     /locus_tag="ACHKW0_004380"
     CDS             9059..9250
                     /locus_tag="ACHKW0_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfgG family protein"
                     /protein_id="HFO7477093.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         2439 a         2302 c         2277 g         2367 t
ORIGIN      
        1 aattattttt attctttatt tcgtaccaaa gattttgtca ccggcatcgc cgaggcccgg
       61 aataatgtat ccgtgctcgt tcagtccctg atcaatcgat gcggtataca gttcgacgtc
      121 cgggtgcgct ttttccagcg cagcgatacc ttctggcgca gcgaccagca ccagaacttt
      181 gatgctgctg cagcccgctt ttttcagcag gtcgatggtc gcgataacgg aaccaccggt
      241 tgccagcatt gggtcaacga tcagcgccat acgctcatcg atgttagaaa ccagtttctg
      301 gaagtacggt accggctcca gcgtttcttc attacggtac ataccgacaa cgctgatgcg
      361 cgcgctcgga acgttttcca gcacaccgtc catcatacca agacccgcac gcagaattgg
      421 cacaacggta attttcttac ctttgatctg gtcgatttct accgggccgt tccagccttc
      481 gatagttact ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca ggctacccac
      541 ttcggaagcg agttcgcgaa agcgcttggt gctgatatct tgctcacgca tcagtcccag
      601 cttgtgtttg acgagtgggt gtttgacttc cacgatcttc atactctttc tcctttgagg
      661 ggcagccaca aaaaaaatcg acggattata cctcctttct tcaaggcggc aatattcttt
      721 tcgttgactt tagtcaaaat gataacggtt tgagataaag ttattttata ttcaaatggt
      781 tatgaaagaa gattattcca tccgaaaact aacctttacc ctggcacaag tcttctttcg
      841 ccgcgtgcct gggcaaaaga cgtgcaaaaa ggttgtgtaa agcagtctcg caaacgtttg
      901 ctttccctgt tagaattgcg ccgaatttta tttttctacc gcaagtaacg cgtggggacc
      961 caagcagtga ccgataaaac ctctcttagc tacaaagatg ccggtgttga tattgacgcg
     1021 ggtaatgctc tggttggaag aatcaaaggc gtagtgaaga aaacgcgtcg tccggaagtg
     1081 atgggcggtc ttggcggctt cggtgcgctg tgtgcattgc cgcaaaaata tcgtgaacct
     1141 gtgctggttt ccggtactga cggcgtaggt accaagctgc gtctggcgat ggacttaaaa
     1201 cgtcacgaca ccattggtat tgatctggtc gccatgtgcg ttaatgacct ggtggtgcaa
     1261 ggtgcagagc cgctgttttt cctcgactat tacgcaaccg gaaaactgga tgttgatacc
     1321 gcttcagcgg tgatcagcgg tatcgcggaa ggatgtctgc aatcgggctg ttcactggtg
     1381 ggtggcgaaa cggcagaaat gccggggatg tatcacggtg aggattacga tgtcgcgggt
     1441 ttctgcgttg gcgtggtaga aaaatcagaa atcatcgacg gctctaaagt cagcgacggc
     1501 gatgtgctga ttgcactcgg ttccagcggt ccgcactcga acggctattc gctggtgcgc
     1561 aaaattcttg aagtcagcgg ttgtgatccg caaaccaccg aacttgatgg taagccatta
     1621 gccgatcatc tgctggcacc gacccgcatt tacgtgaagt cagtgctgga gttgattgaa
     1681 aaggtcgatg tgcatgccat tgcgcacctg accggcggcg gcttctggga aaacattcca
     1741 cgcgtattgc cagataatac tcaggcagtg attgatgaat cttcctggca gtggccggaa
     1801 gtgttcaact ggctgcaaac ggcaggtaac gttgagcgcc atgaaatgta tcgcaccttc
     1861 aactgcggcg tcgggatgat tatcgccctg cctgctccgg aagtggacaa agccctcgcc
     1921 ctgctaaact ccaacggtga aaacgcgtgg aaaatcggta tcatcaaagc ctctgattcc
     1981 gaacaacgcg tggttatcga ataatgaata ttgtggtgct tatttccggc aacggaagta
     2041 atttacaggc aattattgac gcctgtaaaa ctaacaaaat taaaggcacc gtacgggcag
     2101 ttttcagcaa taaggccgac gcgttcggcc ttgaacgcgc ccgccaggcg ggtattgcaa
     2161 cgcatacgct catcgccagc gcgtttgaca gtcgtgaagc ctatgaccgg gagttgattc
     2221 atgaaatcga catgtacgca cccgatgtgg tcgtgctggc tggttttatg cgcattctca
     2281 gcccggcgtt tgtctcccac tatgccgggc gtttgctgaa cattcaccct tctctgctgc
     2341 cgaaatatcc cggattacac acccatcgtc aggcgctgga aaatggtgat gaagagcacg
     2401 gtacatcggt gcatttcgtc accgatgaac tggacggtgg cccggttatt ttacaggcga
     2461 aagtcccggt atttgctggt gatacggaag atgacatcac cgcccgcgtg caaacccagg
     2521 aacacgctat ttatccactg gtgattagct ggtttgccga tggtcgtctg aaaatgcacg
     2581 aaaacgccgc gtggctggat ggtcaacgtc tgccgccgca gggctacgct gccgacgagt
     2641 aatgccccgt agttaaagcg ccagctctgc cgctggcgtt tttcaattca cctgtaaatc
     2701 gcaagctcca gcagtttttt tccccctttt ctggcatagt tggacatctg ccaatattgc
     2761 tcgccataat atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg taatgggtca
     2821 ggaaaagcta tacatcgaaa aagagctcag ttggttatcg ttcaatgaac gcgtgcttca
     2881 ggaagcggcg gacaaatcta acccgctgat tgaaaggatg cgtttcctgg ggatctattc
     2941 caataacctt gatgagttct ataaagtccg cttcgctgaa ctgaagcgac gcatcattat
     3001 tagcgaagaa caaggctcca actctcattc ccgccattta ctgggcaaaa ttcagtcccg
     3061 ggtgctgaaa gccgatcagg aattcgacgg cctctacaac gagctattgc tggagatggc
     3121 gcgcaaccag atcttcctga ttaatgaacg ccagctctcc gtcaatcaac aaaactggct
     3181 gcgtcattat tttaagcagt atctgcgtca gcacattacg ccgattttaa tcaatcctga
     3241 cactgactta gtgcagttcc tgaaagatga ttacacctat ctggcggtgg aaattatccg
     3301 tggcgatacc atccgttacg cgctgctgga gatcccatca gataaagtgc cgcgctttgt
     3361 gaatttaccg ccagaagcac cgcgtcgacg caagccgatg attcttttgg ataacattct
     3421 gcgttactgc ctcgatgata ttttcaaagg cttctttgat tatgacgcgc tgaatgccta
     3481 ttcaatgaag atgacccgcg atgccgaata cgatttagtg catgagatgg aagccagcct
     3541 gatggagtta atgtcttcca gtctcaagca gcgtttaact gctgagccgg tgcgttttgt
     3601 ttatcagcgc gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac tgactatttc
     3661 ccgctacgac tccatcgtcc ccggcggtcg ttatcataat tttaaagact ttattaattt
     3721 ccccaatgtc ggcaaagcca atctggtgaa caaaccactg ccgcgtttac gccacatttg
     3781 gtttgataaa gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg atgtgttgct
     3841 ctattatcct tatcacacct ttgagcatgt gctggaactg ctgcgtcagg cttcgttcga
     3901 cccgagcgta ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt cacgcatcat
     3961 cgactcgatg atccacgccg cacataacgg taagaaagtc accgtggtgg ttgagttaca
     4021 ggcgcgtttc gacgaagaag ccaacattca ctgggcgaag cgcctgaccg aagcaggcgt
     4081 gcacgttatc ttctctgcgc cggggctgaa aattcacgcc aaactgttcc tgatttcacg
     4141 taaagaaaac ggtgaagtgg tccgttacgc acacatcggg accgggaact ttaacgaaaa
     4201 aaccgcgcgt ctttatactg actattcgtt gctgaccgca gatgcgcgta tcaccaacga
     4261 agtacggcgg gtatttaact ttattgaaaa cccataccgc ccggtgacat ttgattattt
     4321 aatggtgtca ccgcaaaact ctcgccgtct gttatatgaa atggtagacc gcgaaatcgc
     4381 caacgcgcag caagggctgc ccagtggtat caccctgaag ctaaataacc ttgtcgataa
     4441 aggcctggtt gatcgtctgt atgcggcctc cagctccggc gtaccggtta atctgctggt
     4501 tcgcggaatg tgttcgctga tccccaatct ggaaggcatt agcgacaaca ttcgtgccat
     4561 cagtattgtt gaccgttacc ttgaacatga ccgggtttat atttttgaaa atggcggcga
     4621 taaaaaggtc tacctttctt ccgccgactg gatgacgcgc aatattgatt atcgtattga
     4681 agtggcgaca ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca tcatcgacat
     4741 attgttcagc gatacggtca aagcacgtta tatcgataaa gaactcagta atcgctacgt
     4801 tccccgcggc aatcgccgca aagtacgggc gcagttggcg atttacgact acatcaaatc
     4861 actcgaacaa cctgaataac cctatgccaa tacacgataa atcccctcgt ccgcaggagt
     4921 ttgctgcggt cgatcttggt tcaaacagtt ttcacatggt catagcccgt gtggtagatg
     4981 gtgccatgca gattattggc cgcctgaaac agcgggtgca tctggcggac ggcctggggc
     5041 cagataatat gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg tcgctgtttg
     5101 ccgaacggct acaagggttt tctcctgcca gcgtctgtat agtcggcacc catacgctgc
     5161 gtcaggcgct gaacgccact gactttctga aacgcgcgga aaaggtcatt ccctacccga
     5221 ttgaaattat ttccggtaat gaagaagccc gtctgatttt tatgggcgtg gaacataccc
     5281 aaccggagaa aggtcgcaaa ctggttattg atattggcgg cggatctacg gaactggtga
     5341 ttggtgaaaa tttcgaaccg attctcgttg aaagccgccg gatgggttgt gtcagctttg
     5401 cccagcttta cttccctggc ggggtcatca ataaagagaa ttttcagcgc gctcgtatgg
     5461 cggcagcaca aaaactggaa actttaacct ggcaattccg tattcagggc tggaacgtgg
     5521 caatgggcgc ttccggtacc ataaaagccg cccatgaagt gttaatggaa atgggcgaga
     5581 aagacgggat aattaccccg gaacgtctgg aaaaactggt aaaagaagtt ttacgtcacc
     5641 gtaatttcgc atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca gtcttcgttc
     5701 ctggactggc gattttatgc ggtgtgtttg atgctttagc catccgtgaa ctgcgccttt
     5761 ctgacggggc gttacgcgaa ggcgtactgt atgaaatgga aggacgtttc cgtcatcagg
     5821 atgtgcgtag tcgcaccgcc agcagcctcg ccaaccagta tcacatcgac agcgaacagg
     5881 cccgacgagt gctggatacc actatgcaaa tgtacgaaca gtggcgggaa cagcaaccga
     5941 agctggcgca tccgcaactg gaggcgctac tgcgatgggc cgccatgctg catgaggtcg
     6001 ggttgaatat caaccacagc ggtttgcatc gccactccgc ttatattctg caaaacagtg
     6061 acttgccggg ttttaatcag gaacagcagc tgatgatggc gacactggtg cgctatcacc
     6121 gtaaagcgat taagctcgac gatctgccgc gctttacctt gtttaagaag aaacagttcc
     6181 tgccactgat acagctattg cgccttggcg tattactaaa caatcaacgt caggcaacca
     6241 ccacaccgcc aacattgaca ctgattactg atgacagtca ctggacactg cgtttcccgc
     6301 atgactggtt tagtcagaat gcgctggtac tgcttgatct ggaaaaggag caagaatact
     6361 gggaaggcgt ggctggctgg cggttgaaaa ttgaagaaga aagtacacct gaaatcgccg
     6421 cttaatgctt tgcgggccga cgagaaatgt cggcccgcat tattcaggca ctttcgcgaa
     6481 tgggttcgat ttcattcaac gtatcaatta acggctgcgg cttaccaata agataaccct
     6541 gcatataatc gatccccaaa gagagcaccg cctcgcggat ctcttcgttt tcaacgtact
     6601 ctgccactac ccgcattttc ttcattcgcg ccaggtggca aatcgatgcc actatctgat
     6661 aatccagact atttgacaca atattgcgga taaaactgcc gtcaatttta agcagatcgg
     6721 cattcacatt tttaagccgt gcatagctgg cgtagccggt gccgaaatca tcaatcgcaa
     6781 tctggcagcc taattcctga agatgctgca aggtgatttg cgcctgctta acattggtca
     6841 gagcattact ttcggtgact tcaaaaataa gttgccacgc ttcaatctga tatttagcca
     6901 gcaactgact gacttcaaca ggaaaacgag cctgacatac cgaggttgga gacagattaa
     6961 tagcaaaacg gtgagcgggc atcttcgctc tgttttcagc cataaattgc agcgtatgct
     7021 caatgaccca catgtcgata ctcgacgata aaccaaattc gtgcgcgacc ggcaagaagc
     7081 tatcggggcc gatcagttca tcattctcac ctttcatgcg cagaagaatt tcatggtaaa
     7141 catcaccacg cataccggta atcggctggg ccatcaggaa aaaatggttg tgttccagcg
     7201 cctgctgtag tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc aaatacattg
     7261 ccccgcgacg ctgcatattt tccggggcgt tggtcacgat ggaaagttcg gcgaccgtat
     7321 ttagctctcc cagcagcagg tagatatgat tcactggcga gcgcacatag cagtaactga
     7381 cgccaatctg cggttgcatc ggcatgccat cccaaaagaa acgaaattgc ttgagatggc
     7441 tatccagtgc ggtaatgcgc tcctggtgcg attctgtatt cagccgcagc gcgagatcgt
     7501 tacccgaaag ctgataaaca tcttcacccg gttccagcaa gggtgacagc cagtgagaaa
     7561 gtttttgctt gtattgaatc cgcagcatga tgccatagtt cttaaccagc atttccatgc
     7621 cagggatgcg taaataacaa agcgcagacc agggggcatc acgcaacgcg cgattcaggg
     7681 cgcgaacatt tggcagatga accaccgggt ccacatacgc aagccgctga atgcgtctga
     7741 ctaccgctcg ctgacgagtt gccagtacag ccatataatt gacaataaaa gagaatacca
     7801 gataactgga ggaggttatg gtcagctgcg tggtatagcc aggataaatg ggaatgtaat
     7861 tttgatagct gtggatgctg atcatcaaca cgaccgccca gagcagcgaa atcagcttat
     7921 aaccatagcg cattgctccc cacatcatca ggggcaacaa taatgacaag gtataattgg
     7981 tgctaaaaat tgtgcttttt tcatttaacg gcatgcataa cagcaacaat aaagcaccta
     8041 atgccagtag ccagagcgcg aactcttttt tggtgacttt ggcatcaacc tgctgtttta
     8101 attgcgaata atagctacgt aaataaaatg gatttcgcac tacccgaatg atgaagtagc
     8161 acagcgggac accgatcaga ttacccacca gtaaggcctg ataattgatt aaagtcccga
     8221 ggttaaaagg catgacaccg accagatttt ctctgctcgc cagtaatcct acaaatgcag
     8281 caaactggaa aagtatcaga aacagcgttg caggaaacac tatctgccag aatatacgct
     8341 gggaaattaa acgggtatca ccatgtgaaa cgttgttacg ccggggagta aagaccctgt
     8401 aaccgcccca acagagaatg ataatgataa taaaattagc cgttaatgag agcgtttcgt
     8461 aaaaacccag ctctgcatat ttgcggacaa aaatccctaa ggtgatccct ggcaatgccg
     8521 cccagctgaa aaacatcatc atgctgatca tcagtgccaa aggcagataa aagagaaaaa
     8581 cctcaccgga agaaatatgc gcgaaagtat taatgtgggc gaaaatgggc aagattaaag
     8641 aaggcaggaa cagcggaagc ccccaccatt tatcacgtat ttttatataa gttgcattca
     8701 gtttcataga tgctcagcag aatcccccac atcctgaagg aggtgtattc agacaggcat
     8761 cccacctgac ttcgaatgat gattattcat cactatagag agcattgatt ctaagtgtca
     8821 tatgaaagtt ccaattgata tatatcaaac aaaataaccc tgattaatga attattacgt
     8881 ttatcatgtt aattcatcat tattacatca tcattgtaaa taattaaatt aacttccata
     8941 acattaaaat atgtatccac tgacgctttt ttacataacg aagaattgac cattttgtcc
     9001 tgttgtgcct taatgtaagt accgtccaca gcgtgggaca tacttcaagg aaccttttgt
     9061 gagtcaggca accagtatgc gaaaacgaca ccgatttaac agtcgcatga cccgtatcgt
     9121 actgctcatc agctttatct tcttctttgg ccgttttatc tactcgtccg tcggtgcctg
     9181 gcagcaccat cagagcaaaa aagaagctca gcaatccaca ctctccgtcg aatcaccggt
     9241 acaacgttag cggttacctt ctccactttc acagaaccta acggcacttc gctgtcggat
     9301 gcttttgctc tttgggatta tcaaagcggc agatattctt tcatcttaaa ttttacgtct
     9361 ttgtcctgac tgatgtttat cctgt
//
LOCUS       DAZFXT010000167         9117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.167, whole genome shotgun sequence.
ACCESSION   DAZFXT010000167 DAZFXT010000000
VERSION     DAZFXT010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.167"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..784)
                     /gene="aqpZ"
                     /locus_tag="ACHKW0_004381"
     CDS             complement(89..784)
                     /gene="aqpZ"
                     /locus_tag="ACHKW0_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HFO7477094.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRD"
     gene            1210..2868
                     /gene="ybjD"
                     /locus_tag="ACHKW0_004382"
     CDS             1210..2868
                     /gene="ybjD"
                     /locus_tag="ACHKW0_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HFO7477095.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2865..3821)
                     /gene="ybjX"
                     /locus_tag="ACHKW0_004383"
     CDS             complement(2865..3821)
                     /gene="ybjX"
                     /locus_tag="ACHKW0_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HFO7477096.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3972..5087
                     /gene="macA"
                     /locus_tag="ACHKW0_004384"
     CDS             3972..5087
                     /gene="macA"
                     /locus_tag="ACHKW0_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019898 - extrinsic component of membrane
                     [Evidence IEA];
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1990961 - xenobiotic detoxification by
                     transmembrane export across the plasma membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HFO7477097.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            5084..7030
                     /gene="macB"
                     /locus_tag="ACHKW0_004385"
     CDS             5084..7030
                     /gene="macB"
                     /locus_tag="ACHKW0_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990195 - macrolide transmembrane
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HFO7477098.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAETEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVIERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(7103..7327)
                     /gene="cspD"
                     /locus_tag="ACHKW0_004386"
     CDS             complement(7103..7327)
                     /gene="cspD"
                     /locus_tag="ACHKW0_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HFO7477099.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            7477..7551
                     /gene="yljB"
                     /locus_tag="ACHKW0_004387"
     CDS             7477..7551
                     /gene="yljB"
                     /locus_tag="ACHKW0_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YljB"
                     /protein_id="HFO7477100.1"
                     /translation="MNQKFEAVNAIDRNVTDVADANDR"
     gene            7732..8955
                     /locus_tag="ACHKW0_004388"
     CDS             7732..8955
                     /locus_tag="ACHKW0_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7477101.1"
                     /translation="MSRAIHRLSDTLLRKLSGSPTTKSTFFNDGGNLSVRHSTNGLLT
                     WYFTYRSGTGRQISPERLRLGNYPDLSLKAAREKASQCRAWLAEGKNPRYELNHAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGAMPLEKCELRHWLACF
                     DQLAKRNPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRTTELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVMKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVEPHVVEQLTAHQLPGMQRVYNHSRYLDAKRDALNLWVERLDV
                     LAGNHENIVMLKVAK"
BASE COUNT         2337 a         2169 c         2396 g         2215 t
ORIGIN      
        1 tcatgaaagg agcgcaacaa gatgatgaat caacatataa caacatctta aaaaaaggcc
       61 tgacattacg ccaggccttc tgcgttaatt aatcacgctt ttccagcagg gtccggtaaa
      121 tcagaccacc gataatgccg ccgacaattg gcaccaccca gaagaaccac agttgttcta
      181 atgcccagcc gccctggaag atagcaaccg cggtgctgcg cgccgggtta acagaagtgt
      241 tagtcaccgg aatactaatt aagtgaatca gggttaaggc cagaccaata gcgatcggcg
      301 caaaacctgc cggcgcgaat ttgtcggttg cgccgtggat caccaacagg aaacctgcac
      361 tcaataccag ttcaactacc agcgcggaaa gcatggaata accgcctggt gaatgctcgc
      421 cataaccgtt agaagcaaaa ccgctggctg ccgcgtcaaa acccgtttta ccactggcaa
      481 ttaaatacag cagcgccgct gcaacaatac cgccgacaac ctgggcaatt acgtagccaa
      541 cgacttcttt tgccggaaaa cgtccgccag cccataaacc aatagtgacc gccgggttaa
      601 aatgaccacc agaaatatga ccaacagcaa aggccatcgt cagaacggtc agaccgaacg
      661 ccaacgccac gccggcaaaa ccaatgccta attccgggaa gcctgcggcc agtacagcac
      721 taccacagcc accaaaaaca agccagaaag taccaaaaca ttcagctgct aattttctga
      781 acatatccac ctcaattaaa aattgaccct gtgaaaaata tggtcgtttt atagggtcgt
      841 cgtaaaaaag tgacgacgga aataatgcgc ggctatttta aaaacgaagg cgagtcattc
      901 accagataaa taaatccagt aaatttgatt tagggcaaca gcgggttgcc ccatatagtc
      961 atttgtctga ttgacagtgt agtgcaagca aaagatttaa tcctttaggc gtaataaaaa
     1021 ataatttatc atgctaatta tttgattttg ttgtttttgc agacttatca gcaagaggga
     1081 gtataacgcg attattcgct catttttcag acatttgcca tgcttaaatg tgatgtcatc
     1141 acgtattagc aaggcctttc ccgttatact gccagcgtaa aggataagtc acatatttct
     1201 ggaggggata tgattcttga gcgcgttgaa attgtgggtt ttcgcggtat caaccgtttg
     1261 tcgttgatgc tggaacaaaa caacgtcctg attggggaga acgcgtgggg taaatccagc
     1321 ttgctggacg ccttaaccct gctgctatcg ccagaatcag atctctacca ttttgagcgc
     1381 gacgatttct ggttcccgcc gggagatatc aacgggcgag aacatcatct gcatattatt
     1441 ttgaccttcc gcgaatcgct gccaggccga catcgggttc gccgttatcg gccgctggaa
     1501 gcgtgctgga cgccatgcac cgatggctat caccgtattt tttatcgtct ggaaggggag
     1561 agtgcggaag acggcagcgt gatgacactg cgcagttttc tcgataaaga cggacatccg
     1621 attgatgtcg aggatattaa cgatcaggca cgccatctgg tgcgtttaat gccggtgctg
     1681 cgcttgcgtg atgcccgttt tatgcgccgt attcgtaacg gcacggtgcc aaatgtcccc
     1741 aatgtggaag tcaccgcgcg ccagctcgat ttcctcgccc gtgagttgtc ctcacatccg
     1801 caaaatctct ctgatggaca gattcgtcag ggactttccg caatggtgca gttgcttgag
     1861 cattatttct ctgagcaggg ggctggacag gcgcgatatc gtttaatgcg gcggcgggcc
     1921 agcaatgagc aacgaagctg gcgctatctg gatatcatca accggatgat tgaccgacct
     1981 ggagggcgct cgtatcgggt tattttgctc ggcctgtttg caactttatt gcaggcaaaa
     2041 ggcacattgc gactggataa agacgcccgt ccattgttgc tgattgaaga tccagaaacc
     2101 cgtttacacc ccattatgct ttcagttgcc tggcatctgt tgaatcttct gccattgcag
     2161 cgcattgcca ccaccaactc gggtgagttg ctttcgttaa ctccagtgga acatgtttgc
     2221 cgcctggtgc gtgaatcttc tcgtgttgcc gcctggcgtc tggggccaag tggcttgagc
     2281 accgaagaca gccgccgcat ctcttttcac attcgtttta atcgtccgtc atcgctgttt
     2341 gctcgctgct ggttgctggt ggaaggggaa acagaaacct gggttatcaa cgaactggcg
     2401 cgccagtgtg gacaccattt cgatgccgaa gggatcaagg tcattgagtt tgcccagtcc
     2461 gggctaaagc cactggttaa atttgcccgc cgaatgggga ttgaatggca tgtactggtc
     2521 gatggcgatg aagcagggaa gaaatatgcc gctacggtac gcagcctgtt gaataacgat
     2581 cgggaagccg aacgagaaca tttaacggcg ttaccggcgc tggatatgga acattttatg
     2641 tatcgccagg gattttccga tgtgttccac cgcgtggcgc aaatcccgga aaatgtaccg
     2701 atgaatctgc gcaaaattat ctcgaaagcg atccatcgct cttccaaacc cgatcttgcc
     2761 attgaagtgg caatggaggc cggacgtcgt ggtgtggact ccgtaccgac gctgctgaaa
     2821 aaaatgttct cacgcgtgct gtggctggcg cgcggtcgcg cggattaacc gcgaaacatc
     2881 gtggccattt gtggctgaat agcgtcgagc atctcatagc gccgacggta ttcagcccgt
     2941 tttttactgg cgatttcggc aatctctttt cgtgctatct gtgctggaag gcggtaatgg
     3001 cgttcagcat cacatacgcc gccaaccgat tcccagaaag cgttgtaatc ggcatggatc
     3061 ttgccttctt tatcacgata acgcaggctg cggtaaatat gcgtttcatt gctgacggca
     3121 ataatctgct ctacctgcaa acgttgggca aacagacagg ccgcttccat cacgaggcgt
     3181 ttgggaaata gcccgtggca ggctttcgtc gcattctgga tttcctgatg tggaatttcc
     3241 cattttgcgc cttgcagtcc gccaataaac atcgttcttt tcccctgata ttcacacagg
     3301 gtaaacgtga tctctgccag aggaatacct tcgctgttgc gaaacaggat tgtgctgtca
     3361 ccttctttat ccattgagat catcatggtc agctcaagcg tgaactgctc gccgtttttg
     3421 ccttccagct tcgccagttg cagcccgggg gtattcaaat ataagctgaa ttcttccgcc
     3481 gacatacatc cgcggagtaa cgcataatgg taacgtaacg cctccagcaa ttgcttacgg
     3541 ctaagattcg ccgcaaggta agggcgatgc agacgcacag gcagtcgcgg ttggcgcgtt
     3601 aacaacacat tgagattagg ccagtgggaa agttcgttca tccactcaac gcttaaacgc
     3661 ggcataatca acgagcgcag caaaaatttc tggcgaaaac tacggcgatg ccagaattta
     3721 cccggccgac actgtccacg tgccagacta agaaaaagtg acaggctgct gagagattca
     3781 gatggcgtaa aggtccgttc agttagctgc gacatattca tgaaatcaat ggttatacat
     3841 gacgtcgatt tcaccattgc gtatcttaac caaacatcaa tagtgtgatt actaacgtaa
     3901 attttagggt tttgttgata tttcgttgaa gttaatgacc cggattggca tatggagtat
     3961 tcagaatatt tatgaaaaag cggaaaaccg tgaagaagcg ttacgttatt gcgctggtga
     4021 tagtcatcgc cggactgatt acgttatgga gaattcttaa cgcacccgtg ccgacttatc
     4081 agacactgat tgtgcgcccc ggtgatttac agcaaagcgt gctggcgacc ggaaagctgg
     4141 acgcgctgcg taaggttgac gtgggcgcgc aggtcagcgg tcagttgaaa actctgtcgg
     4201 tggcgattgg cgataaagta aaaaaagacc agcttttagg ggttattgat cctgaacagg
     4261 ctgaaaacca gattaaggag gtcgaagcaa cgctgatgga gctacgtgcg cagcggcagc
     4321 aggcggaagc ggagctgaaa ctggcgcggg taacgtattc ccgtcagcaa cgtctggcac
     4381 aaacgcaggc ggtttcacag caggatctcg acaccgccgc gacggagatg gctgtgaaac
     4441 aggcgcaaat tggcaccatt gacgcgcaaa tcaagcgcaa tcaggcttct ctcgatacgg
     4501 ctaaaaccaa tctcgattac actcgcatcg ttgccccgat ggccggggaa gtcacgcaaa
     4561 tcaccactct gcaaggccag acggtgattg ccgcacaaca agcaccgaac attctgacgc
     4621 tggcagatat gagcaccatg ctggtaaaag cgcaggtttc tgaagcggat gtaatccacc
     4681 tgaagccggg gcaaaaagcc tggtttacgg tgcttggcga tccactgacg cgctacgagg
     4741 ggcaaatcaa ggatgtacta ccgacgccgg aaaaggttaa cgacgctatt ttctattacg
     4801 cccgttttga agtccccaac cccaatggtt tgctgcggct ggatatgact gcgcaagtgc
     4861 atattcagct caccgatgtg aaaaatgtgc tgacgatccc tctgtcggcg ttaggcgatc
     4921 cggttggcga taatcgttat aaagtcaaat tgttgcgtaa tggtgaaaca cgcgagcgtg
     4981 aagtgacgat tggcgcacgt aacgataccg atgttgagat tgtcaaaggg cttgaagcgg
     5041 gcgatgaagt ggtgattggt gaggccaaac caggagctgc acaatgacgc ctttgctcga
     5101 attaaaggat attcgtcgca gctatcctgc cggtgatgag caggttgagg tgctgaaggg
     5161 catcaccctc gatatttatg ccggtgagat ggtagcgatt gttggcgctt caggttccgg
     5221 taaatcgacc ctgatgaata ttctcggctg tctggataag gccaccagcg gcacctatcg
     5281 cgtcgccggt caggatgttg ccacactgga cgccgatgcg ctggcgcaac tgcgccgcga
     5341 gcatttcggc tttattttcc agcgttacca tttactttcg catttaaccg ccgagcagaa
     5401 cgttgaagta cccgccgtct atgccggtct tgagcggaaa cagcggctgc ttcgcgccca
     5461 ggggttactg caacgactgg ggctggaaga ccgtacagag tattatccgg cacagctttc
     5521 gggtggtcag caacagcgcg tcagcatcgc gcgggcattg atgaacggcg gtcaggtaat
     5581 tcttgccgat gaaccaaccg gcgcactgga cagccattct ggcgaagagg tgatggcgat
     5641 cctgcatcag ctgcgcgatc gcgggcatac ggtgattatc gtcacccacg atccgcaggt
     5701 cgctgctcag gccgagcggg tgatcgaaat tcgcgacggc gaaattgtgc gcaatcctcc
     5761 cgccattgaa aaagtgaatg ttgctggcgg gacggagcct gttgtcaaca cggtgtctgg
     5821 ctggcggcag tttgtcagcg gttttaacga ggcgctgacg atggcatggc gggcgctggc
     5881 agcgaataaa atgcgtactt tactgaccat gctggggatt attatcggta ttgcgtcggt
     5941 ggtttccatt gtcgtggtgg gtgacgccgc caaacaaatg gtgctggcgg atattcgttc
     6001 tattggtacg aatactattg atgtctatcc cgggaaagat tttggcgatg acgatccgca
     6061 atatcagcag gcgctgaagt acgacgactt aatcgccatc caaaaacaac cgtgggtcgc
     6121 ctcagccaca cctgccgtct cgcaaaacct gcgcctgcgt tataacaatg ttgatgttgc
     6181 tgccagtgcc aatggcgtga gcggcgatta ttttaatgtc tatggcatga ccttcagtga
     6241 aggaaacacc tttaatcagg agcagctgaa cggtcgtgcg caggtcgtgg ttctcgacag
     6301 taatactcgc cgccagcttt tcccccataa agcagatgtg gttggcgagg tgattctggt
     6361 cggcaatatg cccgccagag tcattggtgt ggcggaagaa aaacagtcga tgtttggtag
     6421 cagtaaagtg ctgcgtgtct ggctacctta cagcacgatg tccgggcgag ttatgggcca
     6481 gtcgtggctg aactccatta ctgtcagggt aaaagaagga tttgacagcg ccgagacgga
     6541 acagcaactc acgcgtttac tttcactgcg ccacggaaag aaggatttct ttacctggaa
     6601 catggacggc gtcttgaaaa ctgttgaaaa gaccacacgt actttacaac tgtttctgac
     6661 gctggtggcg gtgatttcgc tggtggtggg cggtattggt gtaatgaata ttatgctggt
     6721 gtcagtgatc gagcggacgc gggaaattgg cattcgcatg gctgtaggtg cgcgagcaag
     6781 cgatgttttg caacagttcc tgatcgaagc cgtactggtt tgcctggtcg gtggcgcgtt
     6841 gggaataact ctgtcactgt taattgcttt caccttgcag cttttcttac ccggctggga
     6901 gattggtttt tcaccgttgg cactgctgct ggcgtttctc tgctcgacgg ccaccgggat
     6961 tttatttgga tggttacccg cacgaaatgc ggcacgactg gatccagtag atgctctggc
     7021 acgagagtaa tttttgagat aaaaatgcca gccgatcggg ctggcatttt gcctttagga
     7081 tgtacacaat gagacagaag agctatgcga ctgccgcttc tacttcgacg ggcacaataa
     7141 cactggcgtg attgcctttt ggcccctggt ggacatcaaa ctgaacggat tgtccagctt
     7201 ttagcgttct gtaaccatcc atctgaatgg tggaataatg agcgaaaata tcttcgccgc
     7261 cgccttcagg gcagatgaaa ccaaaccctt tggcattgtt gaaccactta acagtaccct
     7321 tttccatgct tcgacatcct tcgcaaatct tatacaagta agatggaata aaccggggtc
     7381 agagaggggg ctgttcaaaa cctcgccaac tctagaaata caatttagag aattagggcg
     7441 agccgtcaag cgtttgacag gggccagggg gcaggtatga atcaaaaatt tgaagcagtt
     7501 aacgctattg acaggaatgt gacagatgtc gctgatgcca acgatagatg atagttattt
     7561 atcagttagt gttcgtgggt gttcgtcatg gtgtgggcat ctaatttatt tctatgatta
     7621 taaaggataa tttcgttttt cttgttcgcc cttgttcgtc acagttcgcc cttgtacgat
     7681 gcacggtgta gtcaatggtg tagttaattt tgatgaccgg aaacacgaaa catgagcagg
     7741 gcaattcata gacttagcga cactctttta cgaaaattaa gcggatcacc aaccacaaaa
     7801 agcacctttt ttaatgatgg tggcaacctt agcgtaaggc attcaaccaa tggcctgtta
     7861 acctggtatt tcacttacag gtccggaact ggtaggcaga tatcacctga acgtctgagg
     7921 ctgggaaatt atccagattt gagcctgaaa gcagccaggg aaaaagcgtc gcaatgtcgc
     7981 gcatggctgg ctgaggggaa aaatccgcgc tatgaactta atcatgctgt acaggacgcg
     8041 ttagcccccg tgacggttaa ggaagcgctc acttactggc ttgaatcgta cgcaaaggaa
     8101 aagcgcactg actacgaatc actgaaaagc cggatcaata aacacataat tagccagatt
     8161 ggagctatgc cactggaaaa atgcgagcta cgccactggc tggcctgttt tgaccagctt
     8221 gctaagcgta atccggtatc agccggattt ttgctacagg tatgcaaaca ggcgcttaag
     8281 tactgccgaa aacgacgata cgcaatcagc aacgttcttg atgatatggt tgtcggtgat
     8341 gttgggaaaa aagcagaaat aagcgagcgc gtactaacaa acaaggagct tggggaatta
     8401 ctacgcgccc ttgatgaaaa aatttaccca ccgtactaca gcgccctgat tcgcctcctg
     8461 attgtcttcg gatgtcgtac cactgagcta agacgctctg aggtccagga atgggatttt
     8521 aaagaaatgc tctggaccgt gccgaaagaa cacagcaaaa cgaaggtagc aatattcagg
     8581 ccaataccgg aagcgatctt gccgtttgtc atgaagctgg tggagcagaa caggcacacg
     8641 ggcttattgc tcggagaact gaaagggcaa tcatccgtat cagagtacgg aagaacggca
     8701 cacagacgca ttaatcaagc cccctggacg ttgcacgaca tccggcacac gtttacaacg
     8761 atgctgaacg atttaggcgt ggaaccgcat gttgtggagc agcttaccgc gcaccagttg
     8821 ccaggaatgc aacgagtcta taatcactcc cgttatcttg atgcaaaacg tgacgctctt
     8881 aatctatggg ttgagcgcct cgatgtgctg gctggaaatc atgaaaatat tgttatgctg
     8941 aaggtggcca aatgaaatta cctaaaagac tctattatcc gttacctgaa gcggcagaaa
     9001 agttaggctg tgcaataaaa gatctttatc attacgcata tataggtgct cttaatattt
     9061 ctgtatatat accaaaaatt gatgctaaaa aattcaattt ttcattatgt catttta
//
LOCUS       DAZFXT010000168         9100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.168, whole genome shotgun sequence.
ACCESSION   DAZFXT010000168 DAZFXT010000000
VERSION     DAZFXT010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.168"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..589)
                     /locus_tag="ACHKW0_004389"
                     /pseudo
     CDS             complement(1..589)
                     /locus_tag="ACHKW0_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558160.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     misc_feature    complement(192..308)
                     /locus_tag="ACHKW0_004389"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
     gene            1025..1540
                     /gene="ehxC"
                     /locus_tag="ACHKW0_004390"
                     /gene_synonym="hlyC"
     CDS             1025..1540
                     /gene="ehxC"
                     /locus_tag="ACHKW0_004390"
                     /gene_synonym="hlyC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin-activating
                     lysine-acyltransferase EhxC"
                     /protein_id="HFO7477102.1"
                     /translation="MKSNAFDVMGKVAWLWACSPLHKKWPLSVFAINVIPAIQTNQFA
                     LLIKDELPVAFCSWASLDLECEVKYINDVTSLYAKDWMSGERKWFIDWIAPFGHNMEL
                     YKYMRKKYPYELFRAIRLDESSKTGKIAEFHGGGIDKKLASKIFRQYHHELMSEVKNR
                     QDFNFNIEKEN"
     gene            1542..4538
                     /gene="ehxA"
                     /locus_tag="ACHKW0_004391"
     CDS             1542..4538
                     /gene="ehxA"
                     /locus_tag="ACHKW0_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin EhxA"
                     /protein_id="HFO7477103.1"
                     /translation="MTVNKIKNIFNNATLTTKSAFNTASSSVRSAGKKLILLIPDNYE
                     AQGVGINELVKAADELGIEIHRTERDDTAIANQFFGAAEKVVGLTERGVAIFAPQLDK
                     LLQKYQKVGSKIGGTAENVGNNLGKAGTVLSALQNFTGIALSGMALDELLRKQREGED
                     ISQNDIAKSSIELINQLVDTVSSINSTVDSFSEQLNQLGSFLSSKPRLSSVGGKLQNL
                     PDLGSLGDGLDVVSGILSAVSASFILGNSDAHTGTKAAAGIELTTQVLGNVGKAVSQY
                     ILAQRMAQGLSTTAASAGLITSAVMLAISPLSFLAAADKFERAKQLESYSERFKKLNY
                     EGDALLAAFHKETGAIDAALTTINTVLSSVSAGVSAASSASLIGAPISMLVSALTGTI
                     SGILEASKQAMFEHVAEKFAARINEWEKEHGKNYFENGYDARHAAFLEDSLSLLADFS
                     RQHAVERAVAITQQHWDEKIGELAGITRNADRSQSGKAYINYLENGGLLEAQPKEFTQ
                     QVFDPQKGTIDLSTGNVSSVLTFITPTFTPGEEVRERKQSGKYEYMTSLIVNGKDTWS
                     VKGIKNHKGVYDYSKLIQFVEKNNKHYQARIISELGDKDDVVYSGAGSSEVFAGEGHD
                     TVSYNKTDVGKLTIDATGASKPGEYIVSKNMYGDVKVLQEVVKEQEVSVGKRTEKIQY
                     RDFEFRTGGIPYDVIENLHSVEELIGGKHDDEFKGGKFNDIFHGADGNDYIEGNYGND
                     RLYGDDGDDYISGGQGDDQLFGGSGNDKLSGGDGNNYLTGGSGNDELQAHGAYNILSG
                     GTGDDKLYGGGGIDLLDGGEGNDYLNGGFGNDIYVYRQNYGHHTIADEGGKGDRLHLS
                     DISFDDIAFKRVGNDLIMNKAINGVLSFNESNDVNGITFKNWFAKDASGADNHLVEVI
                     TDKDGREIKVDKIPHNNNERSGYIKASNIASEKNMVNITSVANDINKIISSVSGFDSG
                     DERLASLYNLSLHQNNTHSTTLTTTV"
     gene            4588..6708
                     /gene="ehxB"
                     /locus_tag="ACHKW0_004392"
                     /gene_synonym="hlyB"
     CDS             4588..6708
                     /gene="ehxB"
                     /locus_tag="ACHKW0_004392"
                     /gene_synonym="hlyB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter
                     permease/ATPase EhxB"
                     /protein_id="HFO7477104.1"
                     /translation="MMSKCSSHNSLYALILLAQYHNITVNAETIRHQYNTHTQDFGVT
                     EWLLAAKSIGLKAKYVEKHFSRLSIISLPALIWRDDGKHYILSRVTKDSSRYLVYDPE
                     QHQSLTFSRDEFEKLYQGKVILVTSRATVVGELAKFDFSWFIPSVVKYRRILLEVLTV
                     SAFIQFLALITPLFFQVVMDKVLVHRGFSTLNIITIAFIIVILFEVILTGARTYIFSH
                     TTSRIDVELSAKLFRHLLALPVSYFENRRVGETVARVRELEQIRNFLTGQALTSVLDL
                     FFSVIFFCVMWYYSPQLTLVILLSLPCYVIWSLFISPLLRRRLDDKFLRNAENQAFLV
                     ETVTAINTIKSMAVSPQMIATWDKQLAGYVASSFRVNLVAMTGQQGIQLIQKSVMVIS
                     LWMGAHLVISGEISIGQLIAFNMLAGQVIAPVIRLAHLWQDFQQVGISVERLGDVLNT
                     PVEKKSGRNILPEIQGDIEFKNVRFRYSSDGNVILNNINLYISKGDVIGIVGRSGSGK
                     STLTKLLQRFYIPETGQILIDGHDLSLADPEWLRRQIGVVLQENILLNRSIIDNITLA
                     SPAVSMEQAIEAARLAGAHDFITELKEGYNTIVGEQGVGLSGGQRQRIAIARALVTNP
                     RILIFDEATSALDYESENIIMKNMSRICKNRTVIIIAHRLSTVKNANRIIVMDNGFIS
                     EDGTHKELISKKDSLYAYLYQLQA"
     gene            6712..8151
                     /gene="ehxD"
                     /locus_tag="ACHKW0_004393"
     CDS             6712..8151
                     /gene="ehxD"
                     /locus_tag="ACHKW0_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterohemolysin T1SS ABC transporter subunit
                     EhxD"
                     /protein_id="HFO7477105.1"
                     /translation="MRFYMKGLWDLVCRYKTVFSDVWKIRHTLDAPVREKDEYAFLPA
                     HLELIETPVSRRSHFVVWSILLFVIISLLLSVLGKVEVVSVANGKFTHSGRSKEIKPI
                     ENAIVEKIMVKDGSFVKKNDPLVELTVPGVESDILKSEASLLYEKTEQYRYAILSESI
                     QRNELPEIRITDFPGGEDNAGGEHFQRVSSLIKEQFMTWQNRKNQKQLTLNKKIVERD
                     AALARVSLYEHQVSQEGRKLNDFKYLLNKKAVSQHSVMEQENSYIQAKNEHAVWLAQV
                     SQLEKEIELVREELALETNIFRSEIIEKHRKSTDNIVLLEHELEKNRQRKASSFIKAP
                     VSGTVQELNIHTEGGVVTTAETLMIIVPDNDILEVTASVLNKDIGFIQPGQEVVIKVD
                     AYPYTRHGYLTGKVKNITADSVSVPDTGLVFNVIISVDRNDIQGERKKIPVTAGMTVM
                     AEIKTGVRSVISYLLSPLKETINESLRER"
     gene            8218..8343
                     /locus_tag="ACHKW0_004394"
                     /pseudo
     CDS             8218..8343
                     /locus_tag="ACHKW0_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958720.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8343..8504
                     /locus_tag="ACHKW0_004395"
                     /pseudo
     CDS             8343..8504
                     /locus_tag="ACHKW0_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449473.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0042742 - defense response to bacterium
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II-degrading bacteriocin"
     gene            complement(8554..8907)
                     /gene="cmi"
                     /locus_tag="ACHKW0_004396"
     CDS             complement(8554..8907)
                     /gene="cmi"
                     /locus_tag="ACHKW0_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_104216667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M immunity protein"
                     /protein_id="HFO7477106.1"
                     /translation="MKVISMKFIFILTIIALAAVFFWSEDKGPACYQVSDEQARTFVK
                     NDYLQRMKRWDNDVQLLGTEIPKITWEKIERSLTDVEDEKTLLVPFKAEGPEGKRMYY
                     GIYNCEEGYVEYAND"
BASE COUNT         2854 a         1515 c         1972 g         2759 t
ORIGIN      
        1 cgcgccactg tacatctggc cactctgata ccttcccgta acaactgacg ccagacttta
       61 cgcacaccgt atacctggtg attttcatcg tatacgcgct gtatctcttt cttcagccag
      121 tcatcgtgct gcgcacgggc actgcgttta tccggatgat gtcgctgttg ctgacagtgg
      181 taatacgttg acggggcaat atgcagttca ctgcataccg gtccgacccc gtactgctca
      241 cgcagcttat ccagcagcgg cattattttt tccagaggcg gtcgaactcc gccttcgcaa
      301 aataagcgga agcctggcga aggatatcgt tactgcggcg cagttcacga ttttcacgtt
      361 ccagctcttt cagacgctga cgttcagcgg tggtgagtcc accatcacca ctcccggtat
      421 cccgctcatg ctgacgaacc cacacacgca gagtctctgg tgtacagcca atctttgggg
      481 caatggaaca aattgccgcc cattgtgagt catattcgcc ctgactttcc agaaccatac
      541 gaactgcccg ttgacggacc tcgggggaaa aacgtgtatt tttagtcatc ctgtttacct
      601 ctttctcagg gagtttagtc tccaggattc ccggggcggt tcaaggtttg cttttctgtg
      661 taggtttaga ttttgtgttt gcagggtaaa caaaactgat taactcgtca tttcggtaac
      721 tgaaatagtt tgctgtattc tgtttacaat ctctttaata aggcatttgt cacgtggcta
      781 ttcatatgaa aatcatacgt tatcttacaa atcaatcatc tgagtgttat aatataactt
      841 agctgtgata tgtgtaagaa tgtttaggca atatattatt atcgctaatg ttgttttggt
      901 tgggagagat tctccttgcg gtagcttttt gtttttagat gcttcttgct taaaaagata
      961 taatttctgt tcttttatat aggattcttt acatttactg agttttctta tttatttaaa
     1021 cattatgaaa tctaatgctt ttgacgtcat ggggaaggtt gcatggctat gggcctgttc
     1081 tcctctgcat aaaaaatggc cattatctgt ttttgctatt aatgtcatac ctgctattca
     1141 gacaaatcaa tttgctctct taataaagga tgagcttcct gtagcttttt gtagctgggc
     1201 cagtttagat ctggaatgtg aggtaaaata tataaatgat gtaacatctt tgtatgcaaa
     1261 agactggatg tcgggagaaa gaaagtggtt tattgactgg atagctccat ttggacacaa
     1321 catggagtta tataaataca tgaggaaaaa gtatccttat gagttattca gagcaataag
     1381 gttagatgag agcagtaaaa caggaaaaat agctgaattt catggagggg ggatagataa
     1441 aaaactggca tcaaaaatat tcaggcaata ccatcatgaa cttatgtctg aagtaaaaaa
     1501 cagacaagat tttaatttta atattgagaa agaaaactaa tatgacagta aataaaataa
     1561 agaacatttt caataatgcg acattgacta caaaatcagc atttaataca gcatcatcaa
     1621 gcgtacgttc cgctggcaaa aaactcatat tattaatacc tgataattat gaagctcagg
     1681 gcgtgggtat taatgagttg gtcaaagctg ctgatgagct tggaatagaa atacaccgta
     1741 ctgaacgaga tgatacagcg attgcaaacc agttttttgg tgcagcagaa aaagttgtag
     1801 gattaactga acgtggtgtt gcaatattcg caccacaact tgacaaactt ctgcagaagt
     1861 atcagaaagt tgggagtaaa ataggaggaa ccgctgaaaa tgtaggtaat aatctgggaa
     1921 aagccggaac agttctctca gcactacaga attttacggg gattgcttta tcaggcatgg
     1981 ctcttgatga attgctgaga aaacaacggg aaggagagga tataagtcag aatgatattg
     2041 ccaaaagtag tattgaactt attaatcagc ttgtagatac agtatcaagt ataaacagta
     2101 ccgttgattc attttctgag cagcttaacc agcttggctc atttttatcc agtaaacctc
     2161 gcttaagttc tgttggtggg aaattacaaa atttaccaga cctgggctcc ctgggggatg
     2221 ggctggatgt tgtctccgga attctttctg ctgtatcagc aagctttatt ctgggaaaca
     2281 gtgacgcaca tacaggaaca aaagctgcag cgggtataga actgacaact caggttcttg
     2341 gaaatgttgg taaagctgtt tcgcaatata ttctggctca gagaatggca caggggttat
     2401 cgacaacagc tgcaagtgcg ggtctgatca catcggctgt tatgctggct atcagtcctc
     2461 tttctttcct ggctgctgca gataaatttg agcgagctaa gcagcttgaa tcatattctg
     2521 aacgatttaa aaaattgaat tatgaagggg atgctttact cgcagccttt cataaagaaa
     2581 ccggagctat agatgcagcc ctgacaacaa taaatactgt cctgagttct gtatctgcgg
     2641 gagttagtgc agcctccagt gcatccctca taggggcccc gataagcatg ctggtgagtg
     2701 cattaaccgg tacgatatct ggcattctgg aagcatcaaa acaggctatg tttgagcacg
     2761 ttgcagagaa attcgctgct cggatcaatg aatgggaaaa ggagcatggc aaaaattatt
     2821 ttgagaatgg ctatgacgca agacatgctg cgtttttaga agactctctg tctttgcttg
     2881 ctgatttttc tcgtcagcat gcagtagaaa gagcagtcgc aataacccag caacattggg
     2941 atgagaagat cggtgaactt gcaggcataa cccgtaatgc tgatcgcagt cagagtggta
     3001 aggcatatat taattatctg gaaaatggag ggcttttaga ggctcaaccg aaggagttta
     3061 cacaacaagt gtttgatcct caaaaaggga ccatagacct ttcaacaggt aatgtatcaa
     3121 gtgttttgac atttataaca ccaacattta ccccaggaga agaagttaga gaaagaaaac
     3181 agagtggtaa atatgaatat atgacatctc ttattgtaaa tggtaaggat acatggtctg
     3241 taaaaggcat aaaaaatcat aaaggtgtat atgattattc aaaattgatt cagtttgttg
     3301 aaaagaataa caaacactat caggcgagaa taatttctga gctcggagat aaagacgatg
     3361 tggtttattc tggagcaggc tcatcagaag tatttgctgg tgaaggtcat gataccgtat
     3421 cttataataa gacggatgtt ggtaaactaa caattgatgc aacaggagca tcaaaacctg
     3481 gtgagtatat agtttcaaaa aatatgtatg gtgacgtgaa ggtattgcag gaagtcgtta
     3541 aggaacagga ggtgtcagta gggaagcgaa cagagaaaat acaatatcgt gattttgaat
     3601 tcagaaccgg tggaattcct tatgatgtaa tagagaatct tcattctgtt gaagagctca
     3661 ttggcggaaa acatgatgat gaattcaaag gcggtaagtt taatgatata ttccatggcg
     3721 cagatgggaa cgattatatc gaaggtaatt atggtaatga tcgactatac ggcgatgatg
     3781 gggatgatta tatatccgga ggacagggag acgaccagtt atttggtggt agtggaaacg
     3841 ataaattgag tggaggggat ggtaataatt atctgacagg aggaagcggt aatgatgagc
     3901 ttcaggcaca cggagcttat aatattctgt caggtggtac tggtgatgat aaactttatg
     3961 gtggtggtgg tattgatctt ctggatggag gggaaggtaa tgactatctg aatggtggtt
     4021 ttggtaatga tatttatgtt tataggcaaa actatggtca tcatacaatt gcagatgaag
     4081 gaggtaaagg agatcgtttg cacttatctg atattagctt tgatgatatc gcatttaaga
     4141 gagttggaaa tgatcttatc atgaataaag ccattaatgg tgtactttca tttaatgagt
     4201 caaatgatgt caatgggata acatttaaaa actggtttgc gaaagatgcc tcaggagcag
     4261 ataatcatct tgttgaggtt ataacagata aagatggtcg agagataaaa gttgataaaa
     4321 tacctcataa taataatgaa cggtcaggtt atataaaagc cagtaatata gcatctgaaa
     4381 aaaacatggt taatatcacc agtgttgcca atgatattaa taagattatt tcttcagttt
     4441 caggattcga ttcaggtgat gaacgattag catctttata taatttatcc ttacatcaaa
     4501 acaacacaca ctcaacaact ttaacgacaa ctgtctgaga ctaaaagagg aggtgatggc
     4561 cattccagga ggaatggtca tcgttatatg atgagtaaat gtagttctca taatagtctg
     4621 tatgcactga tattgcttgc acaatatcat aatataactg tcaatgctga aactataagg
     4681 catcagtata atacccacac acaagatttt ggggtgactg aatggttact ggcagcgaaa
     4741 tccattggct taaaagcaaa atatgtagaa aaacattttt ccagattgtc aataatttct
     4801 ttacctgcgt tgatatggcg ggatgacggt aagcattata tattgtctcg tgttactaaa
     4861 gattcatcac gctatcttgt ttatgatcca gaacaacatc agtcactaac ttttagtcgg
     4921 gatgagtttg aaaaactgta tcagggaaaa gtcattctgg ttacgtcaag agcaacagta
     4981 gtcggagagt tagctaaatt tgatttttct tggtttatcc cctctgttgt gaaatacagg
     5041 aggattttac ttgaggtgtt aactgtttct gcttttattc agtttcttgc gttaataaca
     5101 cctctttttt ttcaggttgt aatggataag gttttagttc accgggggtt ttcaacgtta
     5161 aatattatca caatagcatt tattatagtg atactttttg aagtgatatt aaccggagcc
     5221 agaacttata ttttctctca tactacaagt cgtattgacg tcgaactgag cgctaagtta
     5281 ttcagacatt tgcttgcatt gcctgtttca tattttgaaa atcgcagggt cggagagacc
     5341 gttgccagag taagggaact ggagcaaatt cgtaattttt taaccggaca agcgttgaca
     5401 tcagttcttg atctattttt ttctgtaata tttttttgtg tcatgtggta ttacagccct
     5461 caattaacac tggttatatt attgtcacta ccttgttatg ttatatggtc attgtttata
     5521 tcacccttat tacgtcgacg tcttgatgat aagtttctca ggaatgcaga aaatcaagct
     5581 tttcttgtcg aaacggtaac agcaataaat acaattaaat ccatggcagt atcaccacag
     5641 atgattgcta catgggataa acaactggcc gggtatgttg cttccagttt cagggtaaat
     5701 ctggttgcaa tgacagggca gcaggggata cagctgatac agaaaagtgt aatggtaatt
     5761 agtctatgga tgggagcaca tcttgtcata tcgggagaga taagcatcgg gcagttaatc
     5821 gcgtttaata tgcttgcagg tcaggtcatt gctcctgtta tcagactggc tcatctttgg
     5881 caggattttc agcaggttgg tatttctgtt gagcgccttg gtgatgtatt aaatacaccg
     5941 gtagagaaaa agtcaggcag aaatatactg ccggaaattc agggggatat cgaatttaaa
     6001 aatgtcagat ttcggtattc ttctgacggt aatgttattt tgaataatat taatttatac
     6061 atatcaaagg gggatgttat cggtatagtt ggccgttctg gttcaggaaa aagtacatta
     6121 acaaaactgc ttcagcgctt ttatatacca gagaccggac agattttaat tgatgggcat
     6181 gatttatcac ttgcagatcc agaatggtta cgacgccaga ttggtgttgt attgcaggaa
     6241 aatatactat taaatcgtag tattatcgat aatattacat tagcttctcc ggctgtatct
     6301 atggaacagg ctattgaggc agccagactt gcaggtgccc atgattttat tacagaacta
     6361 aaagaagggt acaatactat tgttggagaa cagggcgttg gtctttcagg agggcagcgc
     6421 caacggatcg caatagcccg ggctcttgtt acaaatcctc gaattcttat ttttgatgaa
     6481 gcaaccagtg ctcttgatta tgagtcggaa aatataataa tgaaaaatat gtcaagaata
     6541 tgtaagaaca gaaccgtaat tattattgca cacaggttgt caactgtaaa aaatgcaaac
     6601 agaataattg ttatggataa tggctttatt tctgaagatg gcacacataa agagcttatc
     6661 tccaaaaaag acagtttata tgcatattta tatcagttgc aggcataacg tatgcgcttt
     6721 tacatgaaag gtttatggga tttggtttgc cgttataaga cggttttttc tgatgtctgg
     6781 aaaattcgtc acacacttga tgctcctgtg agagaaaaag atgagtatgc atttcttcct
     6841 gctcaccttg aactcattga aacaccagta tccagacgtt ctcattttgt tgtatggagt
     6901 attttattat ttgtaattat atctcttctc ttatctgttc ttgggaaagt tgaagtggtt
     6961 tcagtagcaa atggtaagtt tactcatagt ggaagaagca aagaaataaa accgatagag
     7021 aatgcgattg tagaaaaaat aatggtaaaa gatggctctt ttgtaaaaaa aaatgatcca
     7081 ttagttgaat tgacggtgcc tggtgttgaa tctgatattt taaaatcaga agcatctttg
     7141 ttgtatgaaa aaacagaaca atatcgctac gcaattcttt ctgaatcaat acaacggaat
     7201 gagcttcctg aaataaggat aacagatttt cctggcggag aagataatgc gggaggtgaa
     7261 cattttcaga gggttagctc attaataaaa gagcaattca tgacatggca gaacagaaaa
     7321 aatcaaaagc agctcacatt aaataaaaaa atagttgaac gggatgcagc gcttgctcgt
     7381 gttagcctgt atgagcatca ggtatcacag gaaggaagaa aactcaatga ttttaagtat
     7441 ttgttgaata aaaaagctgt ttctcaacat tcagttatgg agcaggaaaa tagctatatt
     7501 caggcaaaaa atgaacatgc agtctggctt gcacaggttt ctcaacttga aaaagaaata
     7561 gaacttgtgc gggaagaact ggcactggag acgaatatct tcagaagtga aattatcgag
     7621 aagcacagaa aatcaacaga taacattgtg ttgctggagc atgagcttga aaaaaacagg
     7681 cagagaaaag catcatcttt tattaaagct cctgtgagtg gtactgttca ggagttaaat
     7741 atacatacag aaggtggtgt tgtcacaaca gcagaaacgc tgatgattat tgtccctgat
     7801 aatgatattc tcgaagtaac agcatctgta cttaacaagg atatcggttt tatacaacct
     7861 ggacaggagg tcgttattaa agtagatgca tatccatata cacgtcatgg ttaccttaca
     7921 gggaaagtaa aaaatataac tgcagattct gtttctgttc cggatacagg gcttgtattt
     7981 aacgtgatta tatctgttga tcggaatgat atacagggag aaagaaaaaa aatccctgtt
     8041 acagctggaa tgactgttat ggcagaaata aaaaccgggg tgcgtagtgt tatcagctat
     8101 cttcttagtc cattaaagga aactattaat gaaagtttac gtgaacgtta gtatataaat
     8161 aacatattgg ctttttatat gatatagatt tctgaaatga tcttgtaaag attatctatg
     8221 ttatttaaac aaactatgat acagttttta tctttaaaaa gagttaatgg aatggatagt
     8281 attaaattac ttgataaagt tattatctat caacaacaga atgagcaatt atatccagca
     8341 caggcgttgt tcgttcatat gatgataaat acgattttaa cgccagcact caccgtggca
     8401 tcatcggaga gtcgctcaca aggctcgggg cgatgttttc tggtaaagag taccagatac
     8461 tgcttcctgg tgaaattcac attaaagaaa gtggtaagcg ataatataag ggccgggatt
     8521 ccggcctttt tatgcagcct ttataacagt gtattagtca ttcgcatatt caacatatcc
     8581 ctcctcacaa ttgtatatgc cataatacat tctcttaccc tccgggcctt cagctttaaa
     8641 tgggacaaga agtgtttttt catcttcaac atctgttaaa cttctctcaa tcttttccca
     8701 tgtaattttc gggatttctg taccaagaag ttgaacatcg ttgtcccagc gtttcattct
     8761 ttgcaggtaa tcatttttta caaacgttct ggcctgttca tcgctgacct gatagcatgc
     8821 cggaccttta tcttcagacc agaaaaaaac agcagcaaga gcaataatcg ttaaaataaa
     8881 aataaatttc atgctaatta ctttcataaa atctccacaa cttctgtcgt tcattcgata
     8941 taaaaaacat tacgaatata tccgtacaat gtgagcatac tgtgaagtgg ctttaacaat
     9001 tatgaaataa tacaatagat cacatattgc agtggtctct tttttataga agaaatcaca
     9061 attcataatt gtgacttatt atgcatgttt gtttttcagc
//
LOCUS       DAZFXT010000169         9080 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.169, whole genome shotgun sequence.
ACCESSION   DAZFXT010000169 DAZFXT010000000
VERSION     DAZFXT010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9080
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.169"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..343
                     /locus_tag="ACHKW0_004397"
     CDS             <1..343
                     /locus_tag="ACHKW0_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477107.1"
                     /translation="VLPLFLLLSITTGVIPALLTGVMVACLPEKIGSQKNYRCLAGGI
                     GGVVITEIYCAVIVHIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGL
                     DISCPETDSLS"
     gene            392..979
                     /gene="cbrC"
                     /locus_tag="ACHKW0_004398"
     CDS             392..979
                     /gene="cbrC"
                     /locus_tag="ACHKW0_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030153 - bacteriocin immunity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PF03691 family colicin E2 tolerance protein
                     CbrC"
                     /protein_id="HFO7477108.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKGGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(1041..1763)
                     /locus_tag="ACHKW0_004399"
     CDS             complement(1041..1763)
                     /locus_tag="ACHKW0_004399"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HFO7477109.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(1778..2947)
                     /locus_tag="ACHKW0_004400"
     CDS             complement(1778..2947)
                     /locus_tag="ACHKW0_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418175.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HFO7477110.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVLDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVSKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(2974..4590)
                     /gene="bglH"
                     /locus_tag="ACHKW0_004401"
     CDS             complement(2974..4590)
                     /gene="bglH"
                     /locus_tag="ACHKW0_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HFO7477111.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNAIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(4676..6070)
                     /gene="bglB"
                     /locus_tag="ACHKW0_004402"
     CDS             complement(4676..6070)
                     /gene="bglB"
                     /locus_tag="ACHKW0_004402"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HFO7477112.1"
                     /translation="MKAFPETFLWGGATTANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLEAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVSKTRGLSLKK"
     gene            complement(6089..7966)
                     /gene="bglF"
                     /locus_tag="ACHKW0_004403"
     CDS             complement(6089..7966)
                     /gene="bglF"
                     /locus_tag="ACHKW0_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HFO7477113.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLDEKAQQAPENDDKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(8099..8935)
                     /gene="bglG"
                     /locus_tag="ACHKW0_004404"
     CDS             complement(8099..8935)
                     /gene="bglG"
                     /locus_tag="ACHKW0_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HFO7477114.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILG
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
BASE COUNT         2358 a         2273 c         2012 g         2437 t
ORIGIN      
        1 ggtacttcca ttgtttctct tattgtcgat tactacggga gttattcctg cattgttaac
       61 cggcgtgatg gtcgcctgtc tgccggaaaa gatcgggtca cagaaaaatt atcgttgtct
      121 ggctggtggc ataggtggcg tcgttatcac cgagatctat tgtgcagtta ttgtacatat
      181 taagggcatg gcttcctcgg agttgtttga aaacattctt tctggtgaca gtctcgttgt
      241 ccgcatcatt cctgcattgc tggcaggtgt ggtgatgagc agaatcatta cccgtctgcc
      301 cggattggat atttcatgtc ctgaaacaga ctctttaagt taagcgggat actttatctt
      361 tgggctactc aaaagcagac aggatgttac tatgactcaa aatatcaggc cgttacccca
      421 attcaaatat catcccaagc cactggaaac aggcgcattt gaacaggata aaaccgtaga
      481 gtgcgattgc tgtgaacaac agacgtcagt ttattactcg ggtccctttt attgcgttga
      541 tgaagttgaa catctctgtc cgtggtgtat tgcggacggt tctgctgctg aaaagtttgc
      601 aggtagtttt caggatgatg ccagcataga aggtgttgaa tttgagtatg atgaagagga
      661 cgaatttgcc ggtattaaga acacatatcc tgatgaaatg ctgaaagagt tggttgaacg
      721 cacgccaggt tatcatggat ggcaacagga attctggctt gcgcattgtg gcgatttctg
      781 tgcttttatc ggctatgtgg gctggaatga tataaaagat cgcctcgatg aatttgccaa
      841 ccttgaagaa gattgtgaga atttcggtat tagaaattct gatctagcta aatgcctgca
      901 aaagggtggt gattgtcagg gttatctctt ccgctgtctc cactgcggca agctgagact
      961 gtggggtgat ttttcgtagt tatttaaata atgagaacag gccggagcgt aattcataca
     1021 tccggcctta tttcttcagc ttaaccgagc gctaattctg cggcagcggc tttatcagcg
     1081 attaccatca gcgaaggatg cagctgcaac acggaagctg gaacatcttc ggtaaccgga
     1141 ccttgcagaa catttttcag tgcctgcgct ttacctgcgc cactgacaat tatcagcaag
     1201 tttttcgccg ccatgatgct tttcggcccc atcgtcacgt agctgtcagg cactaacgaa
     1261 aagtcgccgc ccaactcacc atgcgccaca atatcaacca tctctccctg gatcgggaac
     1321 tccacggttt gctcatggaa atgggtcgta ttcggcaaat tgccacaaaa atgaccatct
     1381 gcacctaatc ccagcaccac caaatccagg ccgccttcac gcgccagttt ctgatcatgc
     1441 tcgcggtagt tatcaatagt gagcttctgg atattctctt ctttgatccc cgcaggggtg
     1501 aaaaacagat tacgcagatt ggtaatcgtt acaccttctc cctctttgcc gcgaaaggga
     1561 atttcatcga aattatagaa atagcagtta tcgtaccagg gcttaccttt aaccagggtg
     1621 gtgaggtatt catacatgcc tttgggcgtg ctaccggcgg taattgccag gttaacacga
     1681 cgcgtcttcg acatataacc aagcagatgg tgtgccgcga cacggctcat ttcctggtaa
     1741 tcttcggtaa tgattaattt catcttagtg ccttatttta tttaaataat ttctgtacga
     1801 aggctgcata ggcaggccgc cagacatcca tttcatggtt caggccggga tattcctggt
     1861 aatcaaactt aatttttttc tgctcaagct cagttttcag cccggcgata tccttgccgg
     1921 ttacgacatc tttatcacca acgaccacag taaaattacg tagttgctgg ttaatagctg
     1981 ccggatcgtt cagccgggcc gcgacacctt catccggtac ggttgtcgtg gtaacaccac
     2041 tgaatgtggc cagccagcca aagctttcca gatgattcat tcccgatacc agcgcctgat
     2101 acccaccttg tgaaagccct gccagtgcgc gaccatcggc atctttactg acgttaaaac
     2161 gcttgctaat cagcgggata atatcgttca tcagttcgcg atctgccgct ttagcattca
     2221 gcggataaaa gactttacgt ctttcctgag gcacgaaatc ttcgggaata atgcccttcg
     2281 catcggtttc ggtatccggg atcaccacca gcatcggttt aattttccct tcagccagca
     2341 ggttatccat gatttgcggg atacgcccct gatcgatagc ggaacgtccg gtatcaccaa
     2401 aaccgtgata gaaatagagc actggcaaag gctcgcccat gccggtgtat cctggcgggg
     2461 tccagacata catctgacgt tcagattgca atgcgttgga gtggtaagtt atggcgatca
     2521 aatcaccatg cgcaacagaa cgcgtatcca gataactgcc tggcaccaga atcatactgg
     2581 agttaacctg gcgctgaggc ttagtcattg cggtgcctgt atcaatgctg cgtacaccat
     2641 caacattgaa aaaatactcg tacaggttgc ctttcaggat aggtgtgcgc cacgaccaga
     2701 ctcctgcttc gtctttggtc atcgggtgaa tattgtccag aaccggaaca cccaccacta
     2761 cagagacatt ttttgcccca ggggcaaagt agcgaaaggt cacgctgtta tcggcattga
     2821 cctgagtcac atactgtttc accgggatag tgggtgccgg gctggctggc atatccgctg
     2881 ctatggccca ctgagcagaa ataccgctgg caatagccag cgttaaagca gcaattttta
     2941 tattcatcgt aatattcctt tcattaagtt ccattaccac cagatttcag cctgggcacc
     3001 gacagcaaac tggtcgtctt tattgtcttc gaaggtgaag ccatccagtt cgttttccag
     3061 ggctttgata tacgtggcgt agaaacgaat ttccggacgc gaggtcaaca tactggtatt
     3121 gactttaaag gtatggaaga gcgtggtttt ataaccagac tcattaaatt tattactatt
     3181 cgcatctttg ttttgctggg tgaaatagcc cagttcaaca cctgtctggt tatattggtc
     3241 ccaaatatag gccgggcgaa caaccgcacg aatagattcg aaatcagagt gtgcgcctgt
     3301 ttcgtagcta taaatatcgt taccgaagga gtaaacaatc gcgttagcca caatgaaatg
     3361 atcgccgata taggcttcgc cctgtgaagt cagacgaact gctgttccgc cggtgtgatc
     3421 accataataa cgaccattaa aggtggtaaa tggacttgcg ccagcataac ggccaaagtt
     3481 actggcgata gagttattcg caaccaggaa ggagaattcg ttgaagccac ctttatcaaa
     3541 tttctgcgtt aaagatgtgc caaacatcca ggtatctttc cagtcataat agccgttatt
     3601 atcctgatta tctttttccg atgcgctttc gtttgccgtg acataacgac cactcaccat
     3661 taaggtcgct ttatcccata acgggatatc tttatagcgt aaatcaatag cattggtatt
     3721 aatctgctgt ttgttttgca ggctgcgatc gtaatcatcg acatcttcgc gaaccagcgc
     3781 gatatcaatt ttacccggac cgactttcca gttttccaga cctacacccg ctgcggcatc
     3841 agtacgctgc gttttccagt caagcatctg gatttcaatt ttcggcgcac cgtgtttacc
     3901 cacccagaaa tcagcctctg gcgcaaaggg caggaaacct ttggtggtaa cgtacatatc
     3961 ggagaactgc ataaagttct cgccaccggc gttatcgcca aaccagccgg tagagtactg
     4021 ctgaccaacg ttaccatcca tcatcacaac ggcatcaacc cgtttgccgt tttcgttgta
     4081 gacacgttgt tttaactgca aatcaaacca gccggagtat tcgttaccaa agcggcccag
     4141 agaaccaatc gcccatgatt taggtgaacc atgagaggcg gtcccccaac cagaacggta
     4201 atagccgttg taactaaagc caatttcatc tttcacaaac ttactgaaat ctttcagagt
     4261 catggaagaa taaatcgagc ctgtctcact ggcatttttc tcttcatttt tgaccagtac
     4321 agcatcaggt tttgcggcac tactggtcgg gaacggattg gcggcatacc cttgatcatt
     4381 cgtacttacg ctacgattca ccgtcgctgg cgtatatttt ttcttctctt catctttata
     4441 ctttttgagt tcgctttgcg tttctcttaa cgctttttct aataactcaa ggcgatgttc
     4501 caccgttaat gattcagcca atgattgtgc agaaaaggct aacggtgcca ttaataagat
     4561 ggcagaggta ataagatttc gtctaaacat aatatccctt tatggtgcaa agaaagaatt
     4621 aacgtatcgc atccagactg ttctgaatgc gacgataatt aaggtgcttt attggttatt
     4681 tttttaatga cagcccccgc gttttgctca cttctgcata ccatccgaag cttttcttac
     4741 gcgttcttgt gaggcttcct tcgccattat catcacgatc cacataaata aagccgtagc
     4801 gcttagacat ttgtgaatga gatgcactga ctaaatcaat tggcccccaa ctggtgtacc
     4861 ccataatatc cacaccatcg gcaatcgctt catttacctg taccaggtga tcgtttaaat
     4921 aggcaattcg ataatcgtcc tgtatcgaac catccgcttc aacgctgtct tttgcgccta
     4981 atccgttctc gacaataaat aacggttttt gataacgatc ccaaagcgta tttaacagaa
     5041 cccgtaatcc aaccggatca atttgccacc cccactctga acttttcaga tgcggattgg
     5101 ggatcatatt cagtatgttg ccctgcgcat ttttattaat gctttcgtcg tgggaaacac
     5161 aaccagtcat gtaataacta aaagagatga aatctacggt atgttttaaa tcttctgcgt
     5221 cactttcagt catctcaatg gtgatattgt ggtcgcggaa gaaacgctgc atatagccgg
     5281 gatactgacc acgcgcctga acatcaccaa agaacatcca gcgccggttc tcttccatgg
     5341 cctccaacat atcctgtggc tggcaggtga gggggtaaac cagcccgccg agaagcatat
     5401 tgccgatttt cgcttcgggg atcaggctat gacaggcttt aactgcccgc gcactggcaa
     5461 ccagttgatg atggatcgcc tgataaactt ccgcctcgcc actctcttct gccagcccca
     5521 cgcccgtgaa tggcgcgtgt aacgacatat tgatttcatt aaacgtcagc cataacgcta
     5581 ctttatgttg gtagcgggta aagaccgtgc gggcgtaatg ctcgaagtga tcgatgaccg
     5641 cccgattagc ccaaccgccg tagtttttca ccagcccata tggcatttcg taatgggata
     5701 acgttaccag cggcttgatc cccgcctgcg ccatttcatc aaacagccga tcgtaaaacg
     5761 ctaaccccgc ttcattcggt tcggcttcgt cgccctgagg gaaaattcgc gcccaggcaa
     5821 tggaaatacg cagacaggtg aagcccatct cggcaaataa cgcgatatct tccgggtaac
     5881 ggtgataaaa atcgatggcg acatctttga tattctcttt ccccaggatg cgcggttcca
     5941 tttttcccat tacgccatga ggctgtaaat ctgaggtcgt gatccctttg ccatcttcca
     6001 gccaggcacc ttccacctga ttggcagttg ttgcgccacc ccaaagaaat gtttctggaa
     6061 atgctttcat aattaactcc ttttatcgtt agcgaatgat ggataacagc ggctcaccag
     6121 cgcttatctg cgccgtgccg tggggtaata cgtctgtaaa atcatcgcta ttactgatta
     6181 ataccggcgt cgtcagatca aatccggcct cgcgaatagc agggatatca aaagaaatca
     6241 gccgatcgcc tgtattgacc ttgtcaccca cgttgacgtg agcggaaaag aatttgccgt
     6301 ccagttttac ggtgtcgata ccgacatgaa tcaggatctc cacaccatca tctgactcaa
     6361 tgccaatagc gtgtaatgtg gcgaacaacg aagcaattcg acccgcaacc ggagaacgca
     6421 cttcaccaac cgagggcaga atggcgatac ctttacccaa caggccactg gcaaacgtgg
     6481 tatcagcgac gtgaatgagc ggcacaatct ctcccgtcat cggtgaacag ataccgccct
     6541 gctcaggtgg tgtaataacc tctggtgttt tctcttgcgg ggcaccctgc gctggctgac
     6601 gtttagcggt gatgaaatga agcatcaccg taccgacaaa tgcgcaaccg atggcaatga
     6661 caccgccaat aacgctggcc cagacggtga aatcaatccc cgttgacggg atggtttgca
     6721 tgaaggtgaa aatacttggc aaaccaaagg agtaaacttt cgtttgcgcg tagccaatga
     6781 tggtggcccc caatgctcca ctgatacagg cgataacgaa gggatactta cgcggcaggt
     6841 tgacgccata taccgctggt tcggtgatac caaacaggcc cgtcaacgcc gctgatcccg
     6901 ccaccacttt tttctgcgca tcgcgttcgc agaggaagac gccgagcgcc gccccgactt
     6961 gcgccataat ggcgggcatt aacagcggga tcatggtgtc gtagcccagc acggtgaagt
     7021 tattgataca cagcggcacc aggccccagt gcagtccgaa catgacgaag atttgccaga
     7081 aaccgcccat taccgcgccc gcaaatgcag gaaccgcctg ataaagccag agataaccgg
     7141 cggcaatcat ttcgcttatc caggttgata gcggccccac cagcaaaaag gtgacgggtg
     7201 tgataaccat cagacatagc aatggtgtga agaaattttt gattgccgac ggtaaccacg
     7261 cattaagtcg gcgttccaga atgctgcaca accaggcaga aaaaataatg ggaataaccg
     7321 atgacgagta attcaacaat gtgaccggaa tacccaggaa atccagcccc agctcatccg
     7381 ctttttgccc gttctcgaaa gcagtcagaa ttaatggatg cactaacgct ccaccaatca
     7441 ccatggcagt aaatggatta ccgccgaagc gtttccccgc ggtgtatccc aggattatcg
     7501 ggaagaacca aaacaaggca tcactggcgc tgaataaaat taaataagta ccactttgtt
     7561 cggtcgtcca ctgaaaagtg agcgccagag ccagcatacc tttcaggatc ccggttgccg
     7621 ccatcaaacc gatcagaggc gtaaaaatac ctgaaataac ataaacaaag cggtttagca
     7681 gattaccttt atcatcattt tccggtgcct gttgcgcttt ttcgtcaagg cctgccacac
     7741 tgttaaccgc caggaagaca tcggccacat ggttacctat gaccacctga aactggccac
     7801 cgctttccac caccataata ataccggggg tctttttcag tacctctgct tgcgctttgc
     7861 tttcatcctt taacttaaaa cgtaatcgcg ttgcgcaatg catcagacta acaatgttat
     7921 ctgcgccccc gactcctgcg actatttttc tggctaactc cgtcataact tgccctctac
     7981 cgctttgcgg caaaactcaa aaaaaaaacc tgaaaaaaac ggcctgacgt gaatcaagca
     8041 attttttcag gttttgcccg cttagtgcgg taacaatcct ttactcagta ataatatttc
     8101 agtgttcttt gcgcacgcgc tctatattta tggctaaaaa cataatctct gcgggtgaga
     8161 ttttacgttg atactgcaaa ccaataaaaa tggcgatccg ctccgcacat tgccatgctt
     8221 gcgggtaatt ttgttttact gcttgttgta atgactcatc actatcgtta attgaagcat
     8281 gtccaagaat acgccaggat aaaaacttca gatgcgtaac cagtcgctga taactcaagc
     8341 tttcttcctg gtaattaagg ctgaactgaa attttattaa ttgcagcatt tcgcgcatta
     8401 attgcgtgac acctgcaaca tcctccatat ttccgctcat ttgggcactg accagatgca
     8461 tggcaataaa gcccacttca tctttcggta actgcacgcc caaccgttta tcaataatgg
     8521 ttaacgcttc ttcccctagc tggaactctt tcgggtaaag ccgctggata tcccacagca
     8581 gcgggttggg cagtaacacg ttttgctgaa agcgtttaat cgcaaactgg caatggtcag
     8641 ttagcgagat ataaatactg tcctgtaatt ttcccaagcg ctcctgcgct aaagagataa
     8701 tacgatcaca ggttgccatc acctcaagag gaatatgact taagagttcg cttaatcgcc
     8761 cgttcagttc atgactgctc aaggcatact ctttttctat tccacttgag ttaattcttt
     8821 cgccagcgcg tttttgaaag ccaattccgc gccccatgac gactttttcc cgctgttgat
     8881 catcaataac caccacaaca ttattgttga gaattttggt gatttgcatg ttcatagcaa
     8941 ggaccttttt tataaacaaa aaaacccgac ttcaccagta ttctctggtt atgtcaggtt
     9001 ttgcctgcga atgcagtaac aatccagtca tttattaatg gtttttataa cgaacatcca
     9061 ggttcggaaa ttaatttaat
//
LOCUS       DAZFXT010000170         8921 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.170, whole genome shotgun sequence.
ACCESSION   DAZFXT010000170 DAZFXT010000000
VERSION     DAZFXT010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.170"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHKW0_004405"
     CDS             complement(<1..242)
                     /locus_tag="ACHKW0_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7477115.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSI"
     gene            complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHKW0_004406"
     CDS             complement(453..1850)
                     /gene="ccp"
                     /locus_tag="ACHKW0_004406"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HFO7477116.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            2255..3904
                     /locus_tag="ACHKW0_004407"
     CDS             2255..3904
                     /locus_tag="ACHKW0_004407"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005993 - trehalose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HFO7477117.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            complement(3955..4557)
                     /locus_tag="ACHKW0_004408"
     CDS             complement(3955..4557)
                     /locus_tag="ACHKW0_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HFO7477118.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EISRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            5077..5976
                     /locus_tag="ACHKW0_004409"
     CDS             5077..5976
                     /locus_tag="ACHKW0_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7477119.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            6025..7038
                     /gene="yhjD"
                     /locus_tag="ACHKW0_004410"
     CDS             6025..7038
                     /gene="yhjD"
                     /locus_tag="ACHKW0_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HFO7477120.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            7449..8771
                     /locus_tag="ACHKW0_004411"
     CDS             7449..8771
                     /locus_tag="ACHKW0_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7477121.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
BASE COUNT         2192 a         2295 c         2148 g         2286 t
ORIGIN      
        1 ttgatcgaca aatccatcaa tttatggacg ttttcgtcgt cccaggtctg gcagaaagtg
       61 gccaggttct gacgagcgtt gccatcaaga tataattcat cattgataat ctggaatgcg
      121 acatcatcgc gcatttcgtg cagcggaaat cgttttgact ccgcgatagt agaaatggcc
      181 tttgcgccaa aacgtgaatc gagtagttct gagcggaaat ccgttaacag cttctggtcc
      241 atttcgaact ccttaaattt atttgaaggc aataaaaaag taggatttat ccgcaatgga
      301 agcaaggcat tacaggctta atttaaataa caaaatccta agcagaaaaa cgacttttta
      361 taatataaac atttaacatg ataatattaa aaacagactt tattatcgta ataaaaatcg
      421 cccaaacatt cctgttcggg cgatcaaatt aattattgtt tatcctgcat atacggcgtg
      481 tacaccccgt tcagactgtg caggaaagct acgatatcat ccacatcctc ctgcggcagc
      541 tctttgccta cctgatagcg cagcatcagt ttcaccgccc cgtccagcgt cggcacgtca
      601 ccgcgatgga agtacggtgc cgttaaagca acgttacgta aaccgggtac tttctgacgc
      661 aatttatcac gctcttcttt agtcacattc atacgaccaa tatccgccgc cgtaatttcc
      721 ccaaagttaa agtctttttt cagccccaac ggttcaaagg aacgtccgcc gagaataata
      781 ccaccatgac aagttgcaca tttattatct ttaaataatt gatagccttt tttctgttgc
      841 gccgtcagcg cattttcatc tccacgcaac catttatcaa atggggaatc cggcgtaatt
      901 aatgttttct caaattcagc aatggcatca gtaatatttt cgccactgaa accttgcgga
      961 taaacttcga ggaactgcgc tttaagctgc ggatcttttt ccagcttagc aataatttcg
     1021 tcccaggatt tcgacgccat ttcaatcggg ttcaacggcg gtccacctgc ctgatcctgc
     1081 aatgttgccg cacgaccatc ccagaactgc tcaacgttaa atactgagtt aaataccgtc
     1141 ggcgcgttaa tcggcccaac tgcgccacca acaccaatcg atgtttttct gccatcgacg
     1201 ccccccgcat tcaacgcatg gcaatgagcg catgaaatgg tgctatcagc cgataaacgg
     1261 ggatcgtgat acagcgcaaa gcccaacgcc actttttgcg catcggtagg cagtttttgc
     1321 gggatgggct gcaccggttc attgcgatgc tccggagcag tatcattgct ggcgtaatat
     1381 tctgcgcgct gttttgcaat ccaggccagt atttccgccc gctcttcatc actcacctta
     1441 cccgcccagt gtagcgcggt ataacgcgtt ggtggcatag tttcatactg catcacccat
     1501 tcaatcttat tcagatcgct ttgcgaaacg ggtttatcag ccagcagtgc cgcacgcacg
     1561 gcctcgaggt taaaagattt atatccaagc ttaatgtcgt aatccatcaa ctgtttcgcg
     1621 ccaggaatat aataataggc gggtaattct gccgaaggtg tgtggcaata gtcgcatcct
     1681 ttttcgcgga gaaagcctaa aaccttatta ttttcactga cagcagatgc ctgaacatcg
     1741 gcctgtttac tgcgtttatt atcgtggtac cacacataac cagataaccc taaatagcaa
     1801 atcgcgacgc cagccaggcc gatcgcggta atacgtgaga ccattttcat tattcttccc
     1861 tcacggtggt gagttattgt gacccagaaa attttccaga atgctgcaaa cgtctctgcg
     1921 agacaaagcc atcataaaaa acagggagat acgctttttg ataggaatca atcaattaca
     1981 tgaatataaa ctatcaataa gatagcctgt gcctattacc cagatcagga atggcatatc
     2041 atgattgcgg cgagagcagg attgaatgtt ctgaaaatga aaaacaacac gaaatccgta
     2101 tttttataaa ttccatggat attagtgcgt tatatttttt gacaggttaa taaacgtgat
     2161 ccaccgcacg ctttgtcgcc caccaggcgg agcgaatgac tacccttaaa gaaaagcccg
     2221 ataattagcg acgaatttcg gaggttggat ccttatgctc aatcagaaaa ttcaaaaccc
     2281 taatccagac gaactgatga tcgaagtcga tctctgctat gagctggacc cgtatgaatt
     2341 aaaactggat gagatgatcg aggcagaacc ggaacccgag atgattgaag ggctgcccgc
     2401 ctctgatgcg ctgacgcctg ccgatcgcta tctcgaactg ttcgagcatg ttcagtcggc
     2461 gaaaattttc cccgacagta aaacctttcc cgactgcgca cccaaaatgg acccgctgga
     2521 tattttaatc cgctaccgta aagtgcgccg tcatcgtgat tttgacttgc gcaagtttgt
     2581 tgaaaatcac ttctggctgc cggaggtcta ctccagcgag tatgtatcgg acccgcaaaa
     2641 ttccctgaaa gagcatatcg accagctgtg gccggtgcta acccgcgaac cacaggatca
     2701 cattccgtgg tcttctctgc tggcgctacc gcagtcatat attgtcccgg gcggccgttt
     2761 tagcgaaacc tactattggg attcctattt caccatgctg gggctggcgg aaagtggtcg
     2821 ggaagatttg ctgaaatgca tggccgataa cttcgcctgg atgatcgaaa actatggtca
     2881 catccccaac ggcaaccgca cctattattt gagccgatcg caaccaccgg tttttgcgct
     2941 gatggtggag ttgtttgaag aagatggtgt acgcggtgcg cgccgctatc tcgaccacct
     3001 taaaatggaa tatgccttct ggatggacgg tgcagaatcg ttgatcccta atcaggccta
     3061 tcgccatgtt gtgcggatgc cggacggatc gctgctcaac cgttattggg acgatcgcga
     3121 cacgccgcgt gacgaatcct ggcttgagga cgttgaaacc gcgaaacatt ctggtcgccc
     3181 gcccaacgag gtgtaccgcg atttacgcgc gggagcggcc tcaggttggg attactcttc
     3241 ccgttggctg cgtgatactg gtcgtctggc gagcattcgt accacccagt tcatccccat
     3301 cgatctgaat gccttcctgt ttaaactgga gagcgccatc gccaacatct cggcgctgaa
     3361 aggcgagaaa gagacagaag cgctgttccg ccagaaggcc agtgcccgtc gcgatgcggt
     3421 aaaccgttac ctctgggatg atgaaaacgg catctaccgc gattacgact ggcgacgcga
     3481 acaactggcg ctgttttccg ctgccgccat tgtgccgctc tatgtcggca tggcgaacca
     3541 tgaacaggcc gatcgtctgg caaacgccgt acgcagccgg ttactgacac ctggcgggat
     3601 tctggcaagc gagtacgaaa ccggtgaaca gtgggataaa cccaatggct gggcaccgtt
     3661 acaatggatg gcaattcagg gatttaaaat gtatggcgat gaccttctgg gtgatgaaat
     3721 cgcgcgcagc tggctgaaaa cggtgaatca gttctatctg gaacagcaca aaatgatcga
     3781 gaaataccat attgccgatg gtgttccccg cgaaggcggc ggtggcgagt atccgttgca
     3841 ggatgggttt ggctggacta acggtgtggt acgccgttta attggtttgt acggcgaacc
     3901 ataatatttt tacagccagc cgctaacttc ctgctggctg taaaattatc ctcttcagga
     3961 ggagatattt aacatcattg ccgcctgggt gcgatttttc acttccagac ggcgatacag
     4021 ggattccaga tgcgctttta ccgttccggt actgatattc agcgctctgc tgatctcctt
     4081 atttgattcg cccgccgcta acatggttaa aatctcccgc tggcgggcgc ttaacgattt
     4141 gagatcttta atgtcctttt ccggcgtcgt ccgccagtct ccaggcagaa acatcatccc
     4201 catcgccgca ctatttaccg ccaacgcaaa tgtctcgacg gttgaatcac gaggcacaat
     4261 ggccagcaca ttaaaatgaa taacttcctg taaccaccgt ttattgcaat ccgtcgccgt
     4321 gattaacacc ttaacctcag gaaactgcac cacggttttt tgcagcaacc agtagcaaaa
     4381 ctcaccatcc tgatcgccat cgagcataac taaggcttca gggtaacttt ccagcttttg
     4441 ccataactcg tctgcctgac tggccccctg aatactcact cctggaatac gctgctgtaa
     4501 actgattttc attccatgaa taaatattga ctgcctgtca aacatgacta tttgcataac
     4561 tgaatctcca cctgaatacg ttaaaaagac ttaagtagtg gaagggtatt acccgcgaga
     4621 aaaaataaga attcgccatt tggcggtggc cattctacag agatgacgcg tagaaaatag
     4681 ttaccgatat aaatagttac agctaaacgc ctgaaattac atgtcgaggg cactatttaa
     4741 aacaattttg aggatttcct tatattggtg gttagtacgc atgcaattaa aaatgaaact
     4801 ccgcgaccac aagcaaaaat aacaaacggc aatgagacaa aaataagcac aaatagccaa
     4861 cacgtcctct attcacttta aagggaatcg ctgaaaaata cgctctgttt agggggattc
     4921 acctttctca gaaagctatt ccgccctttt cctgctgaga aatcgccaca ttcgtcatga
     4981 caacattgtg aaacccggca ttagatgtta gaaaaaacaa taaacaatgc gatatgcgcg
     5041 ttactgtttc gctgtgaaca acaatcggtc aaagaaatgg ataaaattca cgcaatgcag
     5101 ttgttcatca aagtcgcgga gctggaaagt ttttcccgcg cggcggattt ctttgctttg
     5161 ccaaaaggaa gtgtttcgcg ccagatacag gcactggaac atcaacttgg cacccagctt
     5221 ctccagcgca ccacgcgacg ggtcaaactc acgccagaag gcatgaccta ttatcaacga
     5281 gcaaaagatg tgttgagtaa tctcagcgaa ctggacggtc tgtttcaaca ggatgccacc
     5341 agtatcagcg gtaaattacg catcgacatc ccgccaggaa tcgcgaaaag cctgttactg
     5401 ccgcgcctgt cggaatttct ctatctgcat ccgggaattg agctggaact gagtagccat
     5461 gaccgtccgg tagatattct tcatgatggt tttgattgcg tgatacgcac tggcgcgtta
     5521 ccggaagatg gcgttatcgc ccgtcccctc ggcaaactga ccatggtcaa ctgtgccagt
     5581 ccgcactatc tgacgcgctt tggttatccg caaagccccg atgatctgac ttcacacgca
     5641 atagtgcgtt atacaccgca cctgggtgta catccgttag gttttgaggt tgccagcgtt
     5701 aatggcgtcc agtggtttaa gtctggcggc atgttgacgg taaacagtag cgaaaactat
     5761 ctcgccgccg gtcttgccgg tctggggatt attcagatcc cgcgcattgc cgtgcgcgaa
     5821 gccctgcgtg ccgggcggct tattgaagtg ttgccaggct accgtgccga gccgctctcc
     5881 ctttcgctgg tttacccgca gcgtcgggag ctttcccggc gtgtaaacct gtttatgcag
     5941 tggctggctg gcgtaatgaa agagcacctg gactgaccga ctatactttt taagaatgac
     6001 cacgacagaa ggacaaaaga gcggatgacg caggaaaacg agatcaaacg tcccacccag
     6061 gatctggagc acgagccgat taagcagttg gataatagcg agaaaggcgg caaagttagc
     6121 caggcgctgg aaaccgtcac caccactgcc gaaaaagtcc aacgccaacc ggttattgcg
     6181 cacctgattc gcgcgacaga acgctttaac gatcggctgg gtaaccagtt tggtgcggct
     6241 atcacctatt tctcattttt atcgatgatc ccgattttga tggtgtcgtt tgccgcaggg
     6301 ggctttgtgc tggcctccca tccgatgttg ctacaggata tcttcgacaa aattctgcaa
     6361 aacatcagcg atccgacgct agccgccacg ttgaaaaaca ccatcaacac cgccgttcag
     6421 cagcgtacga ctgtagggct tgtcggcctg gcggtggcgc tttattctgg catcaactgg
     6481 atgggtaacc tgcgtgaagc gattcgtgcc cagtcgcgcg atgtctggga acgctcgccg
     6541 caagatcagg agaagttctg ggtaaaatat ctgcgtgatt ttatttcact gattggtttg
     6601 ttaattgcgc tgattgtgac gctgtcgatc acctcggttg ccggttcggc gcagcaaatg
     6661 attattagcg ccctgcacct gaacagcatt gagtggctga aaccgacgtg gcggttgatt
     6721 ggcctggcga tttccatctt cgccaactat ctgcttttct tctggatctt ctggcggttg
     6781 ccgcgccacc gtccgcgcaa aaaagccctg attcgcggaa catttctggc tgctattggt
     6841 tttgaagtga ttaaaatcgt gatgacctac accttgccat cgctgatgaa atccccctct
     6901 ggcgcagcat ttggttccgt gctggggctg atggcatttt tctacttctt cgcccgtttg
     6961 acgctgtttt gcgcggcgtg gattgccacc gccgaatata aagacgaccc gcgaatgcca
     7021 gggaaaacgc agccttaaaa taggccggat gcggcgtaaa cgccttatcc ggcatacgat
     7081 tttattcggc ctggctcccc gtaggccgga taagatgcgc cagcatcgca tccggctata
     7141 atgcgcacat aacctcttga aactcttccc cagagcctct tcagccatct atttgggagc
     7201 aaacaatttc attccaactc ataaccccag catataaatc cagttggtaa cttttattta
     7261 acctgaaacc agttttatcc actatttata aaattatgtg aagcatttca tagaagaaaa
     7321 atcactggcc taaacattat cccctttttg cctggttttt gaccatttcc gcgatttgtt
     7381 acacattgaa atatcacttt tgctgtgcgt aatatggcta ttcgttagcc aaaaaataag
     7441 aaaagattat gcaagcaaca gccacaacac tcgaccacga gcaagaatac acgccgatca
     7501 actcgcgtaa taaagtcctt gtcgcctctc tcattggcac ggccattgag ttcttcgact
     7561 tttacattta cgccactgcg gccgttattg tgtttccgca tatcttcttc ccgcagggcg
     7621 atccaacggc agcaacgcta cagtcgctcg ccaccttcgc catcgccttc gtcgcgcgcc
     7681 ccattggctc tgccgttttt ggtcattttg gcgatcgcgt tgggcgtaaa gcgacgctgg
     7741 tcgcctcgtt gctaacgatg gggatttcga ccgtggtgat tggtctgctg ccgggctatg
     7801 ccacgattgg tattttcgcc ccgctgctgc tggcgctggc tcgatttggt cagggtctgg
     7861 gcttaggcgg tgaatggggc ggcgcggcgc tgctggcgac tgaaaacgcc ccaccgcgca
     7921 aacgtgcact gtatggctcc ttcccacagc tgggcgcacc gattggcttc ttctttgcca
     7981 acggcacttt cttgctgctt tcctggctac tgaccgacga gcagtttatg agctggggct
     8041 ggcgtgtgcc atttatcttc tcggcggtgc tggtcattat cggcctgtat gttcgcgtgt
     8101 cgctgcatga gtcgccggtg tttgagaaag tcgctaaagc gaaaaaacag gtgaagatcc
     8161 cgctgggtac gctgctgacc aaacatgttc gcgtaaccgt actgggtacg ttcattatgc
     8221 tggcaaccta tacgctgttt tacatcatga cggtctactc tatgaccttt agtaccgccg
     8281 ccgcgccagt tgggcttggc ctgccgcgta acgaagtgtt gtggatgctg atgatggcag
     8341 ttattggttt tggcgtgatg gtgccagtcg ccggattact ggcagatgcc tttggtcgcc
     8401 gtaaaagtat ggtaattatc accacgctga tcatcctgtt cgcgctgttc gcctttaacc
     8461 cgctgcttgg ttctggcaac ccgattctgg tttttgcctt cctgctgctg gggttaagtc
     8521 tgatggggct gaccttcggg ccgatgggcg cgctgttacc agagctgttc ccaacagaag
     8581 tgcgttacac cggagcatcg ttctcttaca acgtagcgtc gattctcggg gcttccgttg
     8641 caccatatat cgcagcctgg ttgcaggcta actacgggtt aggtgcggtg gggttatatc
     8701 tggcggcgat ggctggcttg acgttaatcg ccctgctgct gacccatgag acgcgacatc
     8761 agtcgttgta agtatcgtgt gggcagccac cctcacccca gccctctccc tgaaagggag
     8821 agggagaaaa gcggagcgag atgttatatc gcgaacataa cagccctccc ccagaaagtg
     8881 aacggaaaag cggtactcgt tgtttcaaca cgcacaaaac a
//
LOCUS       DAZFXT010000171         8839 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.171, whole genome shotgun sequence.
ACCESSION   DAZFXT010000171 DAZFXT010000000
VERSION     DAZFXT010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.171"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..525
                     /locus_tag="ACHKW0_004412"
     CDS             <1..525
                     /locus_tag="ACHKW0_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477122.1"
                     /translation="FQYIYNDNIFSELWGVGLFVTLIVLNIVFNLFFRLLFILSKMIL
                     MSRHIRKMQKKLKQTSVPDFIYYSIEKHNKYPIYVLHMPSNNIIEIGYNIINTDLVIG
                     EKIHFRTLSNRSLYFNLTQPPLIATIKDDVFCTLHDYYNHNNETKSTIDNYISKIKDN
                     HNTPWLLNNENVQE"
     gene            complement(1396..1536)
                     /locus_tag="ACHKW0_004413"
     CDS             complement(1396..1536)
                     /locus_tag="ACHKW0_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7477123.1"
                     /translation="MPQKTIIVGMLCLTMLLTVWVLHASPCEFRVSFMWSEIAAFLQC
                     KP"
     gene            complement(1838..2101)
                     /locus_tag="ACHKW0_004414"
     CDS             complement(1838..2101)
                     /locus_tag="ACHKW0_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO7477124.1"
                     /translation="MKPNIHPEYRTVVFHDTSIDEYFRIGSTIKTDRVIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVDAKKGG"
     gene            2524..2649
                     /locus_tag="ACHKW0_004415"
                     /pseudo
     CDS             2524..2649
                     /locus_tag="ACHKW0_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
     gene            2714..2974
                     /locus_tag="ACHKW0_004416"
                     /pseudo
     CDS             2714..2974
                     /locus_tag="ACHKW0_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3024..3263
                     /locus_tag="ACHKW0_004417"
                     /pseudo
     CDS             3024..3263
                     /locus_tag="ACHKW0_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052685.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3311..3928)
                     /gene="ureG"
                     /locus_tag="ACHKW0_004418"
     CDS             complement(3311..3928)
                     /gene="ureG"
                     /locus_tag="ACHKW0_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HFO7477125.1"
                     /translation="MNIIKQPLRVGVGGPVGSGKTALLEALCKSMRDTWQLAVVTNDI
                     YTREDQRILTEAGALEAERIVGVETGGCPHTAIREDASMNLAAVEALSEKFGNLDLIF
                     VESGGDNLSATFSPELADLTIYVIDVAEGEKIPRKGGPGITKSDFLVINKTDLAPYVG
                     ASLEVMERDTLRMRGERPWGFTNLKSGEGLQNIIAFIEEQGMLGK"
     gene            complement(3940..4614)
                     /locus_tag="ACHKW0_004419"
     CDS             complement(3940..4614)
                     /locus_tag="ACHKW0_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HFO7477126.1"
                     /translation="MPTPEKRLRLMQLASNSLPVGGYSWSQGLEWAVEAGWVEDSAAF
                     EHWQQLQMEQSFFAVDLPLLARLYRACEAGDPDSAGRWTAYLLACRETRELRDEERNR
                     GAAFTRLLVDWQPDCPAEWRKLCQQSQLTGMAWLGVRWQISVSDLALSLGYSWIESAV
                     MAGVRLVPYGQLAAQQLIMRLCARYAANMDSALATPDHAIGSATPLASIASARHETQY
                     SRLFRS"
     gene            complement(4615..5079)
                     /gene="ureE"
                     /locus_tag="ACHKW0_004420"
     CDS             complement(4615..5079)
                     /gene="ureE"
                     /locus_tag="ACHKW0_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HFO7477127.1"
                     /translation="MLYLTRRVETPAQTTASVTLPVDMRVKSRIKVTLNDGRQAGLLL
                     PRGLLLRDGDILSNENGDEFIKVIAADEAVSVVRCADPFMLAKACWHLGNRHVPLQIM
                     PGELRYHHDHVLDDMLRQFGLDVDFAHLPFEPEAGAYASKSHAHNHDQEHSH"
     gene            complement(5089..6759)
                     /gene="ureC"
                     /locus_tag="ACHKW0_004421"
     CDS             complement(5089..6759)
                     /gene="ureC"
                     /locus_tag="ACHKW0_004421"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HFO7477128.1"
                     /translation="MFGPTTGDKIRLADTELWIEVEDDLTTYGEEVKFGGGKVIRDGM
                     GQGQMLSAGCADLVLTNALIIDYWGIVKADIGVKDGRIFAIGKAGNPDIQPNVTIPIG
                     VSTEIIAAEGRIVTAGGVDTHIHWICPQQAEEALTSGITTMIGGGTGPTAGSNATTCT
                     PGPWYIYQMLQAADSLPVNIGLLGKGNCSNPDALREQVAAGVIGLKIHEDWGATPAVI
                     NCALTVADEMDVQVALHSDTLNESGFVEDTLTAIGGRTIHTFHTEGAGGGHAPDIITA
                     CAHPNILPSSTNPTLPYTVNTIDEHLDMLMVCHHLDPDIAEDVAFAESRIRQETIAAE
                     DVLHDLGAFSLTSSDSQAMGRVGEVVLRTWQVAHRMKVQRGPLPEESGDNDNVRVKRY
                     IAKYTINPALTHGIAHEVGSIEVGKLADLVLWSPAFFGVKPATIVKGGMIAMAPMGDI
                     NGSIPTPQPVHYRPMFAALGSARHRCRVTFLSQAAAANGVAEQLNLHSTTAVVKGCRT
                     VQKADMRHNSLLPDITVDSQTYEVRINGELITSEPADILPMAQRYFLF"
     gene            complement(6785..7105)
                     /locus_tag="ACHKW0_004422"
     CDS             complement(6785..7105)
                     /locus_tag="ACHKW0_004422"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0035550 - urease complex [Evidence IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HFO7477129.1"
                     /translation="MIPGEYKVKPGYIELNIGRATCSIIVENHGDRPIQVGSHYHFAE
                     VNPALKFDRQKARGYRLNIAAGTAVRFEPGQKREVDLVALSGARIVHGFRGDIMGELE
                     ANDE"
     gene            complement(7114..7416)
                     /locus_tag="ACHKW0_004423"
     CDS             complement(7114..7416)
                     /locus_tag="ACHKW0_004423"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HFO7477130.1"
                     /translation="MELTPREKDKLLLFTAALLAERRLARGLKLNYPESVALISAFIM
                     EGARDGKSVAALMEEGRHVLSREQVMEGIPEMIPDIQVEATFPDGSKLVTVHNPII"
     gene            complement(7426..8205)
                     /locus_tag="ACHKW0_004424"
     CDS             complement(7426..8205)
                     /locus_tag="ACHKW0_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HFO7477131.1"
                     /translation="MLDLRFQRLHGKTTLTTRHHVGPLTVQRPFYPEEETCHLCLLHP
                     PGGIVGGDELIINAIIDSDCHTLITMPGASKFYRSSGAQAHLQQNLTLCNNATLEWLP
                     QDSIFFPGAHATLHTVFHLSSSSTLLAWDLLCLGRPVIGETFSHGTLANRLEIWVDGS
                     PLLIERLHVANGELTCVARKPWVGTMLFYLGNETQLEDIREKLTPLENYAGATLTDGL
                     LTVRFLSDDNLRCQQVMRDIWQFMRPQLTDKSPVLPRIWLT"
     gene            8586..>8839
                     /locus_tag="ACHKW0_004425"
     CDS             8586..>8839
                     /locus_tag="ACHKW0_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477132.1"
                     /translation="MTEHWSGHADDNFPFSWGSDSIFMHACLQSYGESICDTVHTFVA
                     DVAPESLTGLQTVYLYTAGLLCDIVFHIEGGPPEMNGIII"
BASE COUNT         2252 a         2202 c         2133 g         2252 t
ORIGIN      
        1 ttccagtata tttacaatga caatatattt tcagaactat ggggagttgg cctgtttgtt
       61 actctgatag tactgaatat agtatttaat ctctttttta gactcttatt cattctctcg
      121 aaaatgatat taatgtcacg acatataaga aagatgcaaa aaaaactaaa acagacgtct
      181 gtgcctgatt ttatttatta ttcaatagaa aaacacaaca aatatcccat atatgtatta
      241 cacatgccat caaacaatat aattgaaata ggctacaata taataaacac cgatctggtc
      301 attggcgaaa aaattcattt caggacacta agcaatcgtt ctttatattt taacctcacc
      361 caacccccat taatcgccac aataaaagat gatgtgtttt gcactcttca tgattactat
      421 aaccacaata atgaaacgaa atccactatt gacaattaca tatcaaaaat aaaggataat
      481 cataataccc catggttatt aaacaacgaa aatgttcagg aataaaaaaa agagacaatt
      541 cccgagataa aacaaaagag cataaacata tgaaccggaa attctatttc aaaaatatcc
      601 tgtggggaat gtgccccatt ataatatcca tattgcatct gatatatgac acaccaacag
      661 aagcatcgat aaaagttgac ttatttctga tactcagtgg cctggccctt ccttgttcaa
      721 tatacctgac aaaaacaatc acactaaaat acacatccat atcattctgg aaaaaacctc
      781 ttttctggtc ttatggtata tatcattttc atcttgttta tataatgaca tgtatagttt
      841 tagcaatacc ttctgcaatt atcgttttat tctttcactt ttatcaaaac atcaccagaa
      901 actggaagtg acaaccgcaa taatgaacat aaaaaggcaa atcagaatgg cagtaaatca
      961 ataaagtacc atttcatatg aatcatcaga aaaacggtgg acgcgacatc cagcacgagg
     1021 caaaacacag caacaaaata agccggatat cgcgccctgt acttttctaa ctcaactccc
     1081 ggcattgtgc aagttttaat cgcaggtgga ttcagagcca cgcccccatt gattcagcct
     1141 gaagacacgt atatccttgg agaaagcagg aatctcattg ctgtgaaatt ccttacagaa
     1201 tcttcagccc cattttatgg ggcttttttt ggtgccccca cgctgttacc acactttcgg
     1261 gatgcgaata aaaaagccca cagtttttac actgcggact tttgtttttt ctttgtgatt
     1321 tgtgtgcaag ggtgcgactt tcgaccgaac ctttggcaac cggatagcgg ggactgttgt
     1381 ccccgctgcg gtttcttacg gtttacactg caagaacgcc gcaatttccg accacatgaa
     1441 gcttaccctg aattcacacg gtgatgcgtg taacacccag acggtcagca acatggtcag
     1501 gcataacatg cccacaatga tcgttttttg cggcatagca cttgctcctt ttacagagag
     1561 gcgctaacct tccacttgct gaaggtagat ggtcagggcc tcgggttaac gtttaagttg
     1621 actcggggcc tttccacatc cggccttcag gtaatccttc cagccgtcag ccgaaaggca
     1681 cccgcgcgta atgtactggt tttcagagag gtccgcaatc ttccggtgac gagatcatct
     1741 gcacacgccc cggtgggacg ggctaacggt atattcaggt gcaggatctc tctgtctggt
     1801 gcgtttttaa attgtcaaac gcaccctggc ggcaaaatca gccacctttt ttcgcatcaa
     1861 caaaacgccc aaaacgctgg gtaaagcgtg caacattacc ttctgaagcg acagttctca
     1921 gtttacctgt ataaaacgga tgcgacttag aagaaacgtc aattgtaaca tatgggtaag
     1981 ttacaccatc cagttcaatt acccggtcag ttttgatcgt cgaaccgatc ctgaagtact
     2041 catcaatact ggtatcgtga aatactactg tacggtattc cggatggata ttcggtttca
     2101 caacagtttt ccccctttaa gtgaaaattc cgcataaagg tacatattca gacggtcaga
     2161 ttcaactgtc tgttcttata acctcactct ctttcagtat tacaatatgt tatataatta
     2221 taaactggac ttcccgaaaa aatccattaa ataagaagcg agccgttgcg gaggcttgct
     2281 ctctccttta acctcatgtg caatatccgt aacgcgctca cataaatcat catctgataa
     2341 tataacaaca cccattattt tattctgacc taaaaacttc ttatgaaata aacgtttacc
     2401 cgacgtatct gatcccgctg tttttacctg tatcctttaa catacttgag aagcccgctg
     2461 aaaaatatac cgtaaaggcg gttctctgtc ttccataaac ctgtcggata agaagggcat
     2521 taaatgaaaa ccaaaaaagt tatgatgatt gcactggtca gctctgcgct ggctctttca
     2581 ggatgtggag ccatgggcac agcgatcaaa aaacgaaatc tggaagtaaa aacccagatg
     2641 agtgaaacct gaggtagcct gagtttaacg gacactcctt cctgaaatag aatggcatca
     2701 gaaggagcta ataatgagca gaaaaaccca acgttactct aaagagttca aagccgaagc
     2761 tgtcagaacg gttcttgaaa atcaactttc gatcagtgaa ggcgcttccc gattatctct
     2821 tcctgaaggc actttaggac aatgggttac cgccgccaga aaagggctcg gtactcctgg
     2881 ttcccgcacg gtggctgaac tggaatctga aattctgcaa ctgcgtaagg cgttaaatga
     2941 agctcgcctt gagcgagata tattaaaaaa agcagctgta gattcaatct gtcaatgcaa
     3001 cacccctttc aattatctct ttcactgccc gcgacggtat cggttatcag tttgacctgc
     3061 tggctaacgg tgaaaccgta ctgcgtgatg catccggcga aaaacgcatc aagggtgaaa
     3121 aagacggtcg tatgctcatg aatgttggcc tgaatgcgga aattcgcgac aacatccgct
     3181 tcggtctgga gtttgaaaaa tcggcatctg gtaagtacaa cgtggataac gcggtgaacg
     3241 ccaacttccg ttattccttc tgataaaaac aaggggccgc ctgccggccc tgatacagag
     3301 gatgttgtga ttacttaccc agcatgccct gctcttcgat aaaggcgatg atgttttgca
     3361 gaccttcacc acttttcagg ttagtgaacc cccatgggcg ttctccgcgc atacgtaaag
     3421 tatcacgctc cattacctcg agggaggcgc ccacgtatgg tgcaaggtca gttttattga
     3481 tcacgaggaa gtcggatttg gtgatccccg gaccgccttt acgcgggatc ttttctcctt
     3541 ctgccacatc tatgacatat atggtaaggt ccgccagttc ggggctgaag gtcgcactca
     3601 ggttgtcgcc gccgctttcc acaaagatca gatcgagatt accaaatttc tcactcagtg
     3661 cttccacggc tgccagattc attgatgcgt cttcacgaat ggctgtatga gggcagcccc
     3721 ccgtttcgac accgacaatg cgttccgctt ccagtgctcc ggcctcagtg agaatgcgtt
     3781 gatcttctct ggtgtagatg tcgttggtga ctacggctaa ttgccaggtg tcacgcatgg
     3841 atttgcagag ggcttccaga agcgcggttt tacctgagcc gaccggaccg ccgacgccga
     3901 cacgcagcgg ttgcttgatt atgttcattt tattttctcc taagaacgga ataatcggga
     3961 atactgggtt tcatgtcgcg cggaagcgat agatgcaagt ggcgttgctg aaccaattgc
     4021 gtgatccggg gttgcaaggg cactgtccat gttcgccgca tagcgggcac acaggcgcat
     4081 aattaactgc tgggcagcca gctggccgta gggtaccagc ctgacgcccg ccatcactgc
     4141 gctttcaatc cagctgtagc caaggctcag ggccaggtct gaaacggata tttgccagcg
     4201 tacgccgagc catgccatac cggttaactg gctttgctgg catagtttgc gccattcggc
     4261 cgggcagtcg ggctgccagt cgaccagaag tctggtgaag gctgcaccgc ggttacgttc
     4321 ttcatcgcgt agctcacggg tttccctgca ggccagcaga taagcggtcc agcgcccggc
     4381 actgtcagga tcgcccgctt cacaggcccg gtatagccgg gcaagcagtg gcaggtcgac
     4441 ggcaaaaaaa ctctgttcca tctgcaactg ctgccagtgt tcaaaggcgg cgctatcttc
     4501 tacccagcct gcttccactg cccactccag tccctgggac cagctgtagc ctccaacagg
     4561 gaggctgtta ctggcaagct gcatcagccg cagacgtttt tccggagtcg gcattcagtg
     4621 gctgtgctcc tgatcgtgat tatgggcgtg cgacttgctg gcgtaagcgc cagcttccgg
     4681 ctcaaacgga agatgtgcaa aatcaacatc cagcccaaac tggcgcagca tatcatcgag
     4741 gacgtgatcg tgatggtagc gcagctctcc gggcattatt tgcagcggaa cgtggcgatt
     4801 gccgagatgc cagcaggctt tcgcgagcat aaaaggatct gcgcagcgca ctacagaaac
     4861 ggcttcatca gcggcgatca ccttaatgaa ttcatcaccg ttttcgttac tgagaatatc
     4921 accatcgcgc agcagcagac cgcgtggcag caacagtcca gcctgacgtc catcgttaag
     4981 ggtgacttta atacggcttt ttacccgcat gtcaacaggg agggtaacgc tggcagttgt
     5041 ctgagccggg gtctccaccc gccgggtcaa ataaagcatt tgtgctcctt aaaacaggaa
     5101 ataacgttgc gccattggca gaatgtccgc cggttcactg gttatcagtt cgccgttgat
     5161 acgcacttcg taggtttgtg aatccacggt tatatcaggc aacaggctgt tgtggcgcat
     5221 atcggctttt tgtactgtgc ggcagccttt taccacagca gttgtgctgt gcaggttaag
     5281 ctgttcagcg acgccatttg ctgctgctgc ctgcgacagg aaagtcacac gacagcggtg
     5341 acgggcactg cccaatgcag cgaacattgg gcgatagtgc accggctgcg gtgtggggat
     5401 agagccgttg atatcaccca tcggcgccat ggctatcatt ccgcctttga cgatagtcgc
     5461 cggttttacg ccaaagaacg ccggggacca gagcaccaga tccgccagtt ttcccacttc
     5521 aatcgagccg acttcatgag caataccgtg ggttaatgcc ggattaatgg tgtatttagc
     5581 gatatagcgc ttcacgcgga cgttgtcgtt atcaccactt tcttccggta acgggccgcg
     5641 ctgaactttc atccggtgtg ccacctgcca ggttcgtaac actacttctc cgacgcgtcc
     5701 catggcctgc gaatcggacg aggtgaggga gaacgcgcca aggtcgtgca ggacgtcttc
     5761 cgcggcaatg gtttcctggc gaatgcgcga ttcggcaaag gctacgtcct cggcgatatc
     5821 cgggtcaaga tgatggcaaa ccatcagcat gtccagatgc tcatcaatag tgttgacggt
     5881 atagggtagc gtcggattgg ttgaggaagg cagaatattg gggtgcgcgc aggcggtgat
     5941 aatatccgga gcatgaccac cacctgcacc ttctgtatgg aaggtatgga tagtgcgccc
     6001 gccgatggca gtcagagtat cctcaacgaa tcctgattcg ttaagcgtat cactgtgtag
     6061 cgcaacctga acgtccattt cgtcggctac agtcagtgcg cagttgatta ccgcaggtgt
     6121 agctccccag tcttcgtgaa ttttgaggcc gataaccccg gccgcgacct gctcacgaag
     6181 cgcatccgga ttggagcaat tgcctttacc cagcaacccg atattgaccg gcaggctgtc
     6241 tgcagcctgc agcatttgat aaatgtacca tggtcctggg gtacaggttg tggcgttaga
     6301 acccgctgtc gggccagtac cgccaccgat catggtggta atgccggatg tcagcgcttc
     6361 ttcagcctgc tgtgggcaga tccagtgaat atgcgtatcg acgccacctg cggtaacgat
     6421 cctgccttct gcggcaataa tttccgtgga tacgccgatt gggatagtga cgttgggttg
     6481 tatatcagga ttaccggctt tgccgatagc aaatatcctt ccatctttga cgccgatatc
     6541 ggctttaacg atcccccagt aatcgatgat cagggcattg gtcagcacca gatcagcgca
     6601 gccggcggag agcatttgcc cctgtcccat accgtcgcgg attactttac cgccgccgaa
     6661 tttgacctct tcgccgtagg tagttaaatc atcttcgacc tcgatccaca gctcagtgtc
     6721 tgccagacga attttatcac cggtggtagg gccgaacatg tcagcatagg cctagcgtga
     6781 aatattactc atcatttgcc tccagctcac ccattatatc tccgcggaaa ccgtgcacga
     6841 tacgggcacc gctcagcgcc accagatcaa cctcacgctt ttggcctggt tcgaagcgca
     6901 ctgcagttcc ggcggcgata ttgagacgat agcctcttgc cttctggcga tcgaacttca
     6961 gtgccgggtt aacctccgcg aagtgataat gggaaccgac ctgaattggt cggtctccgt
     7021 gattttcaac gataatactg caggtagccc ggccgatgtt aagctctata tagccaggtt
     7081 taaccttata ttctccaggg atcatctgtt acctcagatt atcggattat ggacggtaac
     7141 cagcttggag ccgtccggaa aggtggcttc gacctggata tcggggatca tttctggtat
     7201 gccttccatg acctgctcgc gactcaggac atgccgtcct tcttccatca gcgcagcgac
     7261 gcttttgccg tcgcgagcgc cctccattat aaaagcgcta atcagggcca cggattcggg
     7321 atagttaagt ttcaggccgc gggccagacg acgctctgcc agcagtgcag cggtaaacag
     7381 taatagtttg tctttttctc ttggagtcag ttccatagcg ttctcttaag tcagccagat
     7441 ccggggaagt accggtgatt tgtcagtcag ttgcggccgc ataaattgcc agatatcacg
     7501 cattacctgc tggcaacgca gattatcatc acttaaaaag cgaactgtca gtagaccatc
     7561 ggtgagcgtg gctccggcgt agttttccag tggtgtgagt ttttcgcgta tgtcttccag
     7621 ttgcgtttca ttccccaggt agaacagcat cgttcccacc catggtttcc gggcaacgca
     7681 ggtaagttct ccattggcca cgtgcagacg ctcaatcaac agcggagaac cgtcgaccca
     7741 tatctccaga cggttagcga gtgttccgtg gctgaaggtt tctccgatga ccggacgtcc
     7801 aaggcacaga agatcccagg ccagcagtgt actggaggac gacagatgaa acacggtatg
     7861 caacgtggcg tgagcgccgg gaaaaaaaat cgaatcctgt ggcaaccact ccagcgttgc
     7921 gttattgcac agcgtcaggt tttgctgaag gtgcgcctgc gcgccgctgc tgcggtaaaa
     7981 tttactggca ccaggcatgg tgatcagcgt atggcaatca gagtcaatta ttgcgttgat
     8041 gatgagttcg tcaccgccga cgataccacc gggtggatga agcagacaga ggtggcaggt
     8101 ttcttcttcg ggataaaaag gacgctgaac agtgagcgga ccgacatgat gacgggtggt
     8161 gagcgtggtt ttcccgtgca gacgctgaaa acggagatcc agcatggcct gccagccttt
     8221 tttattttgt ggtaacgcgg tactgagcaa tcttacgccc ctgataaata aagataaaac
     8281 gacagtaaag agccggattt ccttgttata taaaagatga tgcctttcca gtgagttttc
     8341 gtctgaataa tacgaatgtt tgttttcgtc atgaagcgtt ccggctcccg gggctataca
     8401 tcgccagaaa agcaatgcat attgtcggga ttatccagag aatcaggcag aaagatccga
     8461 caatgggctg tgattatgcc atctggctca gaatgatatc aaggtgttat ggggataact
     8521 tgctgataca atagcagtat gacagattca ttaatgcgga aactgctcac tgaaacagag
     8581 tgttcatgac tgagcactgg tcgggtcatg cagatgataa tttccccttt tcttggggaa
     8641 gcgacagtat tttcatgcat gcctgtcttc agtcatacgg ggagagcatc tgtgatacag
     8701 tccatacatt tgttgcggac gtcgctcctg agtcactgac tggtctgcaa acagtttatc
     8761 tgtacacggc agggcttctg tgtgatatcg tttttcatat cgaggggggg cctccagaga
     8821 tgaatggaat aataatatt
//
LOCUS       DAZFXT010000172         8816 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.172, whole genome shotgun sequence.
ACCESSION   DAZFXT010000172 DAZFXT010000000
VERSION     DAZFXT010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.172"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(114..1076)
                     /locus_tag="ACHKW0_004426"
     CDS             complement(114..1076)
                     /locus_tag="ACHKW0_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB family protein"
                     /protein_id="HFO7477133.1"
                     /translation="MKKVLARGRVLGNSNSEFARMLEGDGEIKTFTLKSGKQAKFIKT
                     VVLSGDIESKTFVDVSVNGRDQTMLSRESVSDITRTIKLQQFFPAIGREVDGRIEVLD
                     GTRRRAACIYNNVKFEILVTKDSIDIADARQLAKDIQTAREHSLRELGKRLEVTYGAS
                     MTKEEIAQAESISPAKVTRAFQAASVPDEMISVFPIINDISLSDYQFLLKLSEKATNK
                     KMSVEEFMDRVRQKLMALPDYPTIEKSKILAVFRSEGKLLTDRPVKTVETEKLKEFSD
                     RNQFARKKTDPRKRLVVYEFSRISNEVQLEIDEAIKNILRKLPE"
     gene            complement(1073..2278)
                     /locus_tag="ACHKW0_004427"
     CDS             complement(1073..2278)
                     /locus_tag="ACHKW0_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7477134.1"
                     /translation="MKRDYGSVGTIALRASALLQAMSRDIEEQRKEFNLTEYHQTYTR
                     NAVAKLPKLSRRIVELAVKEMEESGYEFNKKRVGNVEQYALTIQNVIDIYAHRQIPKY
                     RDVHKEPYVIFVVNLKGGVSKTVSTVTLAHALRVHPDLLRHDLRILVIDLDPQASSTM
                     FLDHTHSIGTVLETAAQAMLNNLDAETLREAVIRPTMIPGVDVIPASIDDGFVASQWE
                     SLVAEHLPGFKPSEVLRKTIIDRIADDYDFVFIDTGPHLDPFLLNGLAASDLLLTPTP
                     PAQVDFHSTLKYLTRLPEMLERLEEEGVEPRLSASIGFMSKMTSKRDHETSYSLAREV
                     YASNILDSSLPRLDGFERCGESFDTIISANPVSYPGSAEALKKARTEAERFTKAVFDR
                     IEYIRGASK"
     gene            2649..2822
                     /locus_tag="ACHKW0_004428"
     CDS             2649..2822
                     /locus_tag="ACHKW0_004428"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477135.1"
                     /translation="MMEIKAQQFVTSTGRQVLTDNGQQGMGGVAEAIFANCAELDNDQ
                     LNEIIEWVRLYQR"
     gene            2996..3247
                     /locus_tag="ACHKW0_004429"
     CDS             2996..3247
                     /locus_tag="ACHKW0_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001595120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045927 - positive regulation of growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HFO7477136.1"
                     /translation="MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYC
                     VPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLDKYL"
     gene            3244..3531
                     /locus_tag="ACHKW0_004430"
     CDS             3244..3531
                     /locus_tag="ACHKW0_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7477137.1"
                     /translation="MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKL
                     RGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGKRERSDVYNLASERMR"
     gene            3533..3938
                     /locus_tag="ACHKW0_004431"
                     /pseudo
     CDS             3533..3938
                     /locus_tag="ACHKW0_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558235.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4584..5561
                     /locus_tag="ACHKW0_004432"
     CDS             4584..5561
                     /locus_tag="ACHKW0_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HFO7477138.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVTGDKVE
                     LPFVEFAKLCGIPSNQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(5845..6585)
                     /locus_tag="ACHKW0_004433"
     CDS             complement(5845..6585)
                     /locus_tag="ACHKW0_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7477139.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGARPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLR"
     gene            complement(6709..6828)
                     /locus_tag="ACHKW0_004434"
                     /pseudo
     CDS             complement(6709..6828)
                     /locus_tag="ACHKW0_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958721.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
     gene            6900..7162
                     /locus_tag="ACHKW0_004435"
                     /pseudo
     CDS             6900..7162
                     /locus_tag="ACHKW0_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004385766.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
     gene            complement(7372..7725)
                     /locus_tag="ACHKW0_004436"
     CDS             complement(7372..7725)
                     /locus_tag="ACHKW0_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477140.1"
                     /translation="MRQVLNINVGLGSDIPAGHSPSIFEACLHAITASKALNDGGNSQ
                     LSSEVWGYSGASVSFREAFWLATGGDGKILDLPIGKLRKDYFVDTIVIDTNGHNLDII
                     IYDDTTEDILQKLII"
     gene            7931..8128
                     /locus_tag="ACHKW0_004437"
     CDS             7931..8128
                     /locus_tag="ACHKW0_004437"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477141.1"
                     /translation="MHGDLHMGNILFDEKNNRFWPIDFSNSYEHYYSIDKDSKLFVNK
                     MADREFCSILKTLRENAISNI"
BASE COUNT         2459 a         1981 c         1978 g         2398 t
ORIGIN      
        1 atgaaagaat taaaaaaaat actttatatt cagtaaaata agcatgaaaa aagcgtggtg
       61 aaaattgcaa aaaaaggatg ccatttggca tcctgatatt aacccggcat atcctattca
      121 ggcagttttc ttagaatgtt ttttatagcc tcatcgattt ctaactgcac ctcattagat
      181 atgcgggaga actcataaac aacaagacgc tttctggggt cagtcttctt tctggcaaat
      241 tggttacgat cagaaaactc tttcaacttc tcagtttcca ctgtttttac aggccggtct
      301 gtaagcaatt ttccttcaga cctgaagacc gccaggattt tgcttttctc aattgttgga
      361 tagtcaggta aagccatcag tttttggcga actctatcca taaactcttc cactgacatt
      421 tttttgttgg ttgccttttc agacagtttc aacaaaaatt gatagtccga cagcgaaata
      481 tcattgatta tcggaaatac agagatcatt tcatccggta ctgatgccgc ctgaaatgcg
      541 cgtgttactt ttgccggaga gatgctttca gcctgagcga tctcttcttt tgtcatactt
      601 gccccataag taacttcaag gcgttttcct agttctcgga ggctatgctc tctggctgtc
      661 tgtatatctt ttgcaagttg tctcgcgtcg gcaatatcaa tactgtcttt tgtaaccagt
      721 atttcgaatt ttacgttgtt atagatacag gcagcacgac gacgcgtacc gtctaatact
      781 tcaattcgtc catctacttc ccgaccaata gccggaaaaa attgctgaag tttaatcgtg
      841 cgagttatgt cactaactga ctcacgcgaa agcatagtct gatcgcgtcc attaacagat
      901 acatcaacga acgttttaga ttctatatct ccgcttaata caaccgtttt aatgaatttt
      961 gcttgtttcc ccgacttaag agtaaacgtt ttgatctctc cgtcaccttc aagcatgcga
     1021 gcgaactctg aattgctatt gccgagaact cgccctcgag ctaaaacctt tttcatttgg
     1081 atgctccccg aatgtattca atgcggtcaa aaacggcttt agtaaatcgt tccgcttcgg
     1141 tacgagcttt tttcaatgcc tctgcacttc ctggatacga aacagggtta gcactgatga
     1201 tagtgtcaaa agattcacca catcgttcga acccgtccag tctcggaagc gatgagtcaa
     1261 gaatattact cgcatacact tcacgagcca ggctatagga agtttcgtga tcgcgcttac
     1321 tggtcatttt ggacataaac cctatgctcg cactcaggcg gggctcaacg ccttcttctt
     1381 ccaaacgttc tagcatttca ggtaaacgag tcaaatattt gagagttgaa tggaaatcaa
     1441 cttgcgccgg gggagtaggg gtgagaagta aatcactcgc agccaggcca ttgagaagaa
     1501 atggatcaag atgagggcca gtatcaataa aaacaaaatc ataatcatca gcgatacggt
     1561 caatgatggt tttcctgagt acttcagaag gcttaaaacc aggaaggtgt tcagcaacca
     1621 aagactccca ttggcttgct acgaagccgt catcaataga agcagggatg acatcaacgc
     1681 caggaatcat agtgggtcgg ataaccgctt ctcgcagtgt ttcagcatcg agattattta
     1741 gcattgcttg ggcggcagtt tcaaggactg taccaatact atgggtatgg tccaaaaaca
     1801 tagtgctgga cgcctgcggg tcaaggtcga tcacaaggat acgaaggtca tgacgcagca
     1861 aatccggatg tactcgtagt gcatgcgcca gcgttactgt actgacggtt tttgaaaccc
     1921 cacctttcaa gttgacgaca aaaattacgt aaggttcttt atgaacatcg cgatattttg
     1981 gtatctgtcg gtgagcgtaa atatcaataa cgttctgaat tgttaatgca tactgctcga
     2041 cgttgcctac ccgcttttta ttaaattcat agccactttc ttccatttct ttaacggcca
     2101 gttctacgat gcgtcggctc agcttcggca attttgcgac cgcattacga gtatatgttt
     2161 gatgatactc tgtcagattg aattcttttc tttgctcctc aatatcccga ctcatggcct
     2221 gaagtagagc acttgctctg agcgctatgg taccgacact accgtaatct cgtttcatag
     2281 ttttcctctg agatagattc cctgcccata tattgcgtac aaagttcaat cacattgtac
     2341 acagatatga aagaggtaca atatattttg cattaccatt aaattatcgt acgttgcaag
     2401 tcaccattca catcaaaccg catagttaga cattgtctct gagcaggcga ggggttagac
     2461 aagaccgttg ggcgcgtcag agtgttatat taggatacgg gtaggtgaca cccacctctg
     2521 gcacagaacg gacattcatt gatgccatgc cagaatggac gttcaggtta ttccgtccag
     2581 ttctgctggc agcacgagat ctcccctggt atagtgatgc cacggcaaag tgctcaaaca
     2641 gggataatat gatggaaatc aaggctcaac agtttgtcac atcaacgggg cggcaagtcc
     2701 ttactgacaa cggacaacaa ggtatgggcg gcgtggcgga agcaattttt gcaaactgcg
     2761 cggaactgga taacgaccag cttaacgaga tcatcgagtg ggttcggctc tatcagcgct
     2821 gaatgccatc atcaggctgc gcaagcggcc ttttttacgc cccttgttta attcccgcac
     2881 tacctggaca ttcaggtgat tctgtccatc tgtataaaaa acaaccaaaa gacttgttaa
     2941 caggtcatat aaggagtatc tttgagactg gttaaatagt cttgaaaggt ggcctatgcc
     3001 taacattatt ctcagtgata caagcgccag tgtcagcgag ctgaagaaaa atccgatggc
     3061 gacagtcagc gccggtgatg gtttcccggt cgctatcctg aaccgtaatc agcctgcttt
     3121 ttactgtgta cccgcagagc tgtacgaaaa gatgctcgat gccctggacg atcaggagct
     3181 ggttaaactg gtagccgaac gcagcaacca accgctgcat gatgtagacc tagataaata
     3241 cctatgaggt atcaggtaaa attcagggaa gatgcgctga aagagtggca aaaactggac
     3301 aagactattc agcaacagtt tgcgaaaaag ctaaaaaagt gctgtgacaa tccgcatatt
     3361 ccttccgcaa aactgcgcgg gataaaggac tgctacaaaa taaaattacg tgcgtcaggt
     3421 tttcgcctag tctatcaggt gattgacgaa caattaatta tcgctgttgt agctgtgggt
     3481 aaacgtgagc gcagtgacgt ttataatctt gccagtgaaa gaatgagata attcacggcg
     3541 gattatcggt tcagccctct cattaaccgc tgatgctgag ttgatgtgta gggctttgcg
     3601 taatgcactg gaaacacgac cgcgtgatgg tcgcctgctg tttcattcag atcaaggtgt
     3661 tcagtataaa agcaataaat acagaaagtt actttatggg gtaatgcaaa gtatgagtcg
     3721 cagagggaat tgcctggata attcgccgat ggaaagaatc tttcgcagcc ttaaaagtga
     3781 atggcttccg aaaggtggtt atggtgattg ccatgcagta cgcgaataaa tcagtggata
     3841 aatgcttatt acaacgttta tcgacctcat acaaacaatg gtggtttatc gccttgccgt
     3901 atgaagaaaa atggaaacag gttattccgg tgtcctgatt ttatgatcca ctacacattc
     3961 ctgacgaacg ccactgcata aatcacattg cacatcctca gagagtacgc agtataggag
     4021 gggtatatat acattatcgg tatatcaggc gacttctgtc tgtatgttgt cggtgctcgc
     4081 cgcagaccgg tgaccgggcg aagcccgcga accggcgagg aagcgcaatg tatctgtagt
     4141 tacagtgagc aggtacgccc tgaagccctt tcataaaacg gttgtgtgcg ctgtccttcg
     4201 ggaagattta tccacatatc cagaggcccg atccagtaaa cagatccatg aatgatcaat
     4261 aaaggatcca ttaaagatcc ccataccgct gcaaaccttg ccactcatgc tccagaacca
     4321 cgatcacata agctgtggca tgttgatgat aaactgtaac atgctgatga taagctgtat
     4381 tcagtaatac atatactgac gtaagttaat gacataaact atggtcagta caccagactc
     4441 agttgttaaa tacagactgc aggtttttct tcagtcagtt gacgggggtc tgacacacga
     4501 ttttctgttt attcttttac tgtccacagg caggaggctt tctggaaaac gaaaattcag
     4561 acatcaaaaa actgttcggc gaggtggata agtcctccgg tgagctggtg acactgacac
     4621 caaacaataa caacaccgta caacctgtgg cgctgatgcg tctgggcgtc tttgtaccga
     4681 cccttaaatc actgaagaac agtaaaaaaa atacactgtc acgtactgat gccacggaag
     4741 aactgacacg tctttccctg gcccgtgcag agggattcga taaggttgag atcaccggcc
     4801 cccggctgga tatggataac gatttcaaga cttgggtggg gatcattcat tcctttgccc
     4861 gccataacgt gactggtgac aaagttgaac taccttttgt cgagtttgca aaactgtgtg
     4921 gtataccttc aaaccagtca tcacgcaggc tgcgtgagcg catcagccct tccctgaaac
     4981 gcattgccgg taccgtgatc tccttttccc gcaccgatga gaagcacacc cgggaataca
     5041 tcactcatct ggtacagtca gcctactacg atactgaacg ggatattgtt cagttacagg
     5101 ctgatccccg cctttttgaa ctgtaccagt ttgacagaaa agttcttctc cagcttaagg
     5161 cgattaatgc cctgaagcga cgggagtccg cccaggcact ctacacattt atagagagcc
     5221 tgccccggga tccggcaccg atatcgctgg cgcggctgcg tgcacgcctc aatctgaagt
     5281 ctccggtatt ttcccagaac cagacggtca gacgggcaat ggagcaattg cgtgagattg
     5341 gatatcttga ttacactgag atccagcggg gacggacaaa actcttctgt attcactacc
     5401 ggcgtccccg gttaaaagcg ccgaatgatg agagtaagga aaatccgttg ccaccttcac
     5461 ctgcggaaaa agtcagtccg gagatggcgg agaagcttgc cctgcttgag aaactgggca
     5521 tcacgctgga tgacctggaa aaactcttca aatcccgctg aacataaact gtagtcagtg
     5581 aagatcgctc ctttactgac tacagcttat attaaccggg gcagtgagtg gtttgctcac
     5641 tgcagtttat attctgtttc ctgcagtgct gtctgtagct gagctgccat ctgctgtccc
     5701 ttacgtaagt gatcccgtaa cctgatgctg aggcattgct cccttcataa aacatgactt
     5761 actcactaca gcttatatac atgctccagc ttatgttatg tctgttctgc tgaccactgc
     5821 ttatataagg aagcgcgtat gccgtcacct caggggaggc agtgtacgca ggatctccgc
     5881 agcatcccgc ccgtcacctg tgaaaggcac tgccagcgtg gcagccatat ccagtgcaaa
     5941 cactctggta taaacctcca tagaacgtgg atccctgtga ccagccagtg cctgaatgac
     6001 tttccggggc tggcggtgat aaagcatgtg catgatatag ctgtgccgga aggtgtgtgg
     6061 tgtaaccgga atcgaaaagt gtactccgtc agcttcggcc cgtctgacag cctgcttcag
     6121 ccagttgcgc atggtttcgt cggtcacggc ccataatggt tcacgacgac ggggccgggt
     6181 ggtgatcatc cagctttcca tctgcctgac atagcttata tctgtcagcg gaactaggcg
     6241 gacttcatct tttggcgggc gtccgcgtcg cgcacgcact ttttcggaca ggatccgcac
     6301 aaacggtctt gcaccatcca gatcaaatga ttccggcgtc agcatccggg cttcgccaat
     6361 acgcattccg gtattccaga gggtggcgaa cagcatatgg tgacgctgat ccggcatata
     6421 gaagagaagg gcactcactt ccggggccag caggtaggcc ggtgtggccc ccgtggaagt
     6481 ggccattctt ctcagggaga gcgctgttgc aaaatccacc cccggcgcaa tgggtaacag
     6541 ggagacgcgt tctggtgaat tctgcagggg aatgacattg ttcatgagtt atttttcacg
     6601 tagcacacgc actgtttatg gatacaggta ttctgacctg aacgcccgga tccttccacg
     6661 acaataaatc gcagcgcgcg gatccttttg tttgattatc acgcattgtt gtgggtaaaa
     6721 ccaaatacgt aaaggcgcat tttgccgcag gtatcccgtc ttaagccagt catggtgcgg
     6781 gttgagagct tgtttaccgt ttttgggaga attggggtaa tgccaggaga ggcagacatg
     6841 ggtaaacaga gttcagacag gaaagaagtg caggtacgtt acgtaaatcc taaccaagtt
     6901 ttgatatgcg attcgaacgc actgcattat ctgctgtaga aaatagcttc cggatattca
     6961 gtagttctct gggaatatcc cgatggaaaa atatgcagca gataaatgaa tatggcaata
     7021 gagtgcaatt tattcattac gggatgctaa tctacacata aaatatcaga aaagactatt
     7081 aataagaata aacaagccct tatattataa tagtaaaaat atggaggtgg tattttctgg
     7141 atgcaagtta ctcgcgttat aaaatttctt taattttgta aagttttaac cactcaacat
     7201 tatatctact ttttaatttt ctataacaga taacttcaaa gcccatatat ttcgctaaaa
     7261 acattgttat tgataaactg tcagagaagt atttctaccc ttctctgact acctctttga
     7321 taaatattct caccattaac acataccttg catatattat tacgctgcgt gttatattat
     7381 taacttctgc agaatgtcct cagttgtatc atcatatatt atgatatcta aattatgacc
     7441 attagtatca attactatag tatcaacaaa ataatctttc cgaagttttc ctattggtaa
     7501 atccagaatt tttccatcac cgcctgttgc aagccagaaa gcctccctga atgaaacaga
     7561 tgctccggag tatccccaga cctcagaaga taactgagag ttgcccccat cgttaagggc
     7621 tttacttgct gttattgcat ggagacacgc ctcaaatatc gatgggctat gtcctgctgg
     7681 gatatcactc ccaagaccaa catttatatt cagaacctga cgcaagataa atatatctgt
     7741 ttgcaaaaat ataaattaga aagaggactg tgagaaattc cggcagatgc tgagtctata
     7801 agaggcatat gatcatcact gatatgattg cggtatgtaa gaatggtttt tcgtgtcatc
     7861 agatcataat tatataatgc ctgactatcg gtaacaccat atctctcaac gacaacgtca
     7921 cagtgtaatc attcatggag acttacatat gggaaatatc ttatttgatg aaaagaataa
     7981 tcgtttttgg ccgattgatt tttcaaacag ttacgagcat tattattcaa ttgacaaaga
     8041 tagcaaatta tttgtaaata agatggcaga cagagagttt tgctcaatat taaaaacttt
     8101 gcgggaaaat gccataagca atatatagaa aaatgatatt gttggcattg ttcactgata
     8161 ttcttgcggc acatttcaac taaaactcag gagagaagga gtgaatgttg ctcatatatt
     8221 gagagtccaa gaggtcgtcc acctaccaaa acggccaaca atcttggtgt gggcagtagc
     8281 ttgtgataac actttattaa cattgagcgc ttttagtata tatctcctga gtattagcat
     8341 aaacttctaa tataacttaa taatttgaga tcaaatatgt atttcatcgg ttatcatggc
     8401 acgagtgagg cgtcagccca gaatattctt tccgtcggag ttcaggagcg cttccttcct
     8461 ccaacgggac aaatcggcag aggtttctac attgcgaaga tgaacggtac gctaccacag
     8521 tggggcgctg aaaacgcaac cgaattcgcc cgcagaaatc agccgtattt cactcgcatg
     8581 gtcagcttat tgaccggcgc gtcaaataat ccatggcttg ataaagctgc tcagcgaaca
     8641 attttaaaaa tttacactgt ccgtccgcta ttgcattgta aatggaacat catgaacccc
     8701 ggcgacttag aattcttgaa ggccacactc tatgacaacg gtggcccagg tctggatctt
     8761 aagcgcgatt gcaaatggtt gtaccaccag aggaactcca gtacctgcgg gtgaag
//
LOCUS       DAZFXT010000173         8659 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.173, whole genome shotgun sequence.
ACCESSION   DAZFXT010000173 DAZFXT010000000
VERSION     DAZFXT010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8659
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.173"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            162..812
                     /gene="csgD"
                     /locus_tag="ACHKW0_004438"
     CDS             162..812
                     /gene="csgD"
                     /locus_tag="ACHKW0_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm master transcriptional regulator CsgD"
                     /protein_id="HFO7477142.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPENYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            817..1206
                     /gene="csgE"
                     /locus_tag="ACHKW0_004439"
     CDS             817..1206
                     /gene="csgE"
                     /locus_tag="ACHKW0_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HFO7477143.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1231..1647
                     /gene="csgF"
                     /locus_tag="ACHKW0_004440"
     CDS             1231..1647
                     /gene="csgF"
                     /locus_tag="ACHKW0_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HFO7477144.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1674..2507
                     /gene="csgG"
                     /locus_tag="ACHKW0_004441"
     CDS             1674..2507
                     /gene="csgG"
                     /locus_tag="ACHKW0_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HFO7477145.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2571..3062)
                     /gene="ycdZ"
                     /locus_tag="ACHKW0_004442"
     CDS             complement(2571..3062)
                     /gene="ycdZ"
                     /locus_tag="ACHKW0_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HFO7477146.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3164..3718)
                     /gene="ycdY"
                     /locus_tag="ACHKW0_004443"
     CDS             complement(3164..3718)
                     /gene="ycdY"
                     /locus_tag="ACHKW0_004443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HFO7477147.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3742..4479)
                     /gene="ycdX"
                     /locus_tag="ACHKW0_004444"
     CDS             complement(3742..4479)
                     /gene="ycdX"
                     /locus_tag="ACHKW0_004444"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HFO7477148.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4534..5472)
                     /gene="ghrA"
                     /locus_tag="ACHKW0_004445"
     CDS             complement(4534..5472)
                     /gene="ghrA"
                     /locus_tag="ACHKW0_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016618 - hydroxypyruvate reductase
                     [NAD(P)H] activity [Evidence IEA];
                     GO_function: GO:0030267 - glyoxylate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HFO7477149.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            5706..5793
                     /locus_tag="ACHKW0_004446"
     tRNA            5706..5793
                     /locus_tag="ACHKW0_004446"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5740..5742,aa:Ser,seq:gga)
     gene            complement(5943..6785)
                     /locus_tag="ACHKW0_004447"
     CDS             complement(5943..6785)
                     /locus_tag="ACHKW0_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7477150.1"
                     /translation="MSDITISRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISVLTESIGGKTAPDWAMKQDFRCGCWLMEKPETAMKAITRNLDREIWRDLMQRS
                     GMLSLMDTQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFRGLN
                     WNYKTNCPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     GNITLRLDDHIQSVQGKESYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKHYPD
                     MLAV"
     gene            complement(6870..7067)
                     /locus_tag="ACHKW0_004448"
     CDS             complement(6870..7067)
                     /locus_tag="ACHKW0_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7477151.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            complement(7079..7570)
                     /locus_tag="ACHKW0_004449"
     CDS             complement(7079..7570)
                     /locus_tag="ACHKW0_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7477152.1"
                     /translation="MMKLALTLEADSVNVQALNMGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            complement(7567..7941)
                     /locus_tag="ACHKW0_004450"
     CDS             complement(7567..7941)
                     /locus_tag="ACHKW0_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7477153.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            complement(7988..8362)
                     /locus_tag="ACHKW0_004451"
     CDS             complement(7988..8362)
                     /locus_tag="ACHKW0_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7477154.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLNAKGLTCEAD
                     TLGSCGYVYLAVYPTPALATTS"
     gene            complement(8525..>8659)
                     /locus_tag="ACHKW0_004452"
     CDS             complement(8525..>8659)
                     /locus_tag="ACHKW0_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO7477155.1"
                     /translation="RWHGSAEAYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTLN
                     "
BASE COUNT         2233 a         2141 c         2089 g         2196 t
ORIGIN      
        1 gctagattga aatcagatgt aatccattag ttttatattt tacccattta gggctgattt
       61 attactacac acagcagtgc aacatctgtc agtacttctg gtgcttctat tttagaggca
      121 gctgtcaggt gtgcgatcaa taaaaaaagc ggggtttcat catgtttaat gaagtccata
      181 gtattcatgg tcatacatta ttgttgatca ctaaaccgtc tttgcaagcg acagctctct
      241 tgcagcacct taaacaatcg ctggcaatta caggaaaatt acataatatt caacgttctc
      301 tggacgatat atcttcaggc tctattattc ttctggatat gatggaagca gataaaaagc
      361 ttatccatta ttggcaggat actttgagca ggaaaaacaa caatatcaaa atattgttgc
      421 taaatacgcc tgaaaattac ccgtaccgcg acattgaaaa ctggcctcat atcaacggcg
      481 ttttttatgc catggaggat caagaacgtg ttgtcaatgg gttgcaaggc gtcctgcgcg
      541 gcgaatgcta ctttacgcaa aagcttgcca gttacctgat tacacattct ggtaactatc
      601 gttataacag cacggaatca gccctcctta ctcatcggga aaaagagatc ctgaataagc
      661 tgcgtatcgg cgcgtctaat aacgagatcg ctcgttcgtt gttcatcagc gaaaatacgg
      721 ttaaaacgca tctttataat cttttcaaga agatagccgt aaaaaaccgg acacaagcgg
      781 tttcctgggc aaacgataac ctcaggcgat aaagccatga aacgttattt acgctggatt
      841 gtggcggcag aatttctgtt cgccgcaggg aatcttcacg ccgttgaggt agaagtcccg
      901 ggattgttaa ctgaccatac tgtttcatct attggccatg atttttaccg agcctttagt
      961 gataaatggg aaagtgacta tacgggtaac ttaacgatta atgaaaggcc cagtgcacga
     1021 tggggaagct ggatcactat aacggtcaat caggacgtta ttttccagac ttttttattt
     1081 ccgttgaaaa gagacttcga gaaaactgtc gtctttgcac tgattcaaac tgaagaagca
     1141 ctaaatcgtc gccagataaa tcaggcgtta ttaagtacgg gcgatttggc gcatgatgaa
     1201 ttctaaataa aaaattgttc ggaggctgca atgcgtgtca aacatgcagt agttctactc
     1261 atgcttattt cgccattaag ttgggctgga accatgactt tccagttccg taatccaaac
     1321 tttggtggta acccaaataa tggcgctttt ttattaaata gcgctcaggc ccaaaactct
     1381 tataaagatc cgagctataa cgatgacttt ggtattgaaa caccctcagc gttagataac
     1441 tttactcagg ccatccagtc acaaatttta ggtgggctac tgtcgaatat taataccggt
     1501 aaaccgggcc gcatggtgac caacgattat attgttgata ttgctaaccg cgatggtcaa
     1561 ttgcagttga acgtgacaga tcgtaaaacc ggacaaacct cgaccatcca ggtttcgggt
     1621 ttacaaaata actcaaccga tttttaagcc ccagcttcat aaggaaaata atcatgcagc
     1681 gcttatttct tttggttgcc gtcatgttac tgagcggatg cttaaccgcc ccgcctaaag
     1741 aagccgccag accgacatta atgcctcgag ctcagagcta caaagatttg acccatctgc
     1801 cagcgccgac gggtaagatc tttgtttcgg tatacaacat tcaggacgaa accgggcaat
     1861 ttaaacccta cccggcaagt aacttctcca ctgctgttcc gcaaagcgcc acggcaatgc
     1921 tggtcacggc attgaaagat tctcgctggt ttataccgct ggagcgccag ggcttacaaa
     1981 acctgcttaa cgagcgcaag attattcgtg cggcacaaga aaacggcacg gttgccatta
     2041 ataaccgaat cccgctgcaa tctttaacgg cggcaaatat tatggttgaa ggttcgatta
     2101 tcggttatga aagcaacgtt aaatcgggcg gtgttggggc aagatatttt gggatcggtg
     2161 cagacacgca ataccagctc gatcagattg ccgtgaacct gcgcgtcgtc aatgtgagta
     2221 ccggtgagat cctctcctcg gtgaacacca gtaagacgat actttcctat gaagttcagg
     2281 ccggggtttt ccgctttatt gactaccagc gcttgctgga aggggaagtg ggttacacct
     2341 caaacgaacc cgttatgctg tgtctgatgt cggctatcga aacaggggtc attttcctga
     2401 ttaatgatgg tatcgaccgt ggtctgtggg atttgcagaa caaagcagaa cggcagaacg
     2461 acattctggt gaaataccgc catatgtcgg ttccaccgga atcctgacag ataaaaaagc
     2521 cggaattatc atccggcatt ttactgttta agcgaaagcg tgagtgcccc tcacgcttta
     2581 tttttgattt cctgctcacg gtgagccgtc tttgcactac gcgccgccag ccacaggccg
     2641 ctgtttttca ttgcgtaacc aaataccagc ccaagcgcca gcgaaggtaa caccagtttc
     2701 cagtcaccct gcccggcaaa cgtcgcgcat gcgcctataa acgtccccgg aacaaatgaa
     2761 agcagcagct gtttggcctg aatacacatc agaaacgcga caataccggt tatgacataa
     2821 ccgagaattt ccagatgtgg tgccagtgca ctaccgtaaa taatgaccat cgcccacacc
     2881 acgccactaa gcagggttgc agcggagatc gccagccctt tcagcccacc ttgcgggcag
     2941 gcaaagtagg ccgtacagcc caggaagcct gcccagctta gtaagcccag ggaaacagcc
     3001 acccatcccc agataccgga gagaatgcct gttgtgattg caatagagag aagtatgttc
     3061 atgccgcgca tgatagcaga aaacgcggcc atgaatgaga tcgagcacac attttatgca
     3121 acaacaaatt atctgttgca gttatgagtg acgatgatca catttactct tcagaatctt
     3181 cttccagctc gtcccacatt gcactaatgg catcacgggt tagcggtgcc atggtgcgcc
     3241 agaaaggcgt ggttgcatgg gcctccactt tgccgaggaa cgcaccacac cagggtaaca
     3301 gatactcact gaacagtgtt tccagtgctt cgctctcatc ttccgttgac tgatcttcca
     3361 gccaggaagc cgcaagcagc aatgtgccga tgtgatcggc tggcgtatcc gccagtggca
     3421 tccctcgttc ggaaagaaaa gcgcgcactt ccgcttccgt tgcgtcctca acccatgcgc
     3481 tacgatatgg cggcacggca cattcatcgc cgataaacaa cgcattgtaa tcggcagaga
     3541 cttgcgccat atcacagctt ttctgtaaac gcgtcagtaa ctcatcctgc tccagtggcc
     3601 agttcgcagc cagtttcccc tcacgaatca gggtaaacag cggcaccagt aaaggatctt
     3661 gcggttggcg gtaatagagc gaacccagca cacgacagag gatagaaaac tcgttcatta
     3721 atatatttcc attactagta attaaaggtc tgcaaattcc gcaatcggtg ccataccgcg
     3781 agattcaagg aagttcagta agcggcgcgg agaaacattc aaaatgcgct ctggcggaaa
     3841 atctaccgcg tcgaggattt taagacactc ttcaaattcc cccatggtaa acgcggtgtg
     3901 agaatccgag cctaatgcca cccaacctcc agcatcacgt accgctgcgg ctaccgcacg
     3961 acagttgtct tcactgccct tacgtgagtg taaaaatgaa gaattattga tttccagcgc
     4021 cacctgatgt ttcgcggctg cttcagcaac ggctttcaca tctatttcat atttgggatt
     4081 tccgggatgg cttattatat gcacattgcc gctggcgata gtggcgatca tcgcttgtgt
     4141 attggtcgct ttgtcatgtg gtgcaaaaac cggctcatga aaaccggcaa taatcagatc
     4201 cagcgagtca aacattttac cgctgcaatc aatttcacca tcaacatttt taatgttagc
     4261 ttcgatgccg cgcaggatcc ctaccccatc aaccactcgc ggccagatac gcatgttaat
     4321 gaagtgccag tgatgcggag catcttccat atccgggcca tgatcggtga tcgcaaaaag
     4381 tttaatgccc ttttgtttgg cctgggcaat gtaatcactc aatgtgctat atgcatgtgt
     4441 gctggcaacg gtatgcatat gaaggtcgac gggatacata actctctcct gtattcaatt
     4501 cccgccaagg atagcaggaa tcctgatgct ttattagtag ccgcgtgcgc ggtcgacttg
     4561 cccgcagacc ctctcccctt tttcgagctg ggcaatggtg cgagaaatgt actccacagc
     4621 ttcagcggga cgggtaatcg cggcgacatg tggtgttatc gtcacgcgtg gatgttgcca
     4681 gagcggactt tcaggcggta agggttcacg attaaaaaca tccaacattg cgcctttaac
     4741 tttgccgcta tccagcgccg cgagcaggtc atcttccaca acatgaacac cacgcgccag
     4801 gttgaggaga tacgcgccat ccggtaattt ttcgagtaat tgttgattaa taatgccgac
     4861 ggtttcaggg gtattcggta acaaattaat caatacccga cattggctca gaaatgcaga
     4921 cagttcttcc cgtccggcaa agctttgcac gccaggccac gatttacggg ttcgactcca
     4981 gcaacgcagc ggaaagcgcc aggtttgcag actctgagca actttactgc ccagtacgcc
     5041 tgcgcccaaa atgccgatgg taaaatcttc ccgatgatat tcaggcagcg gttgccaatg
     5101 cgaactattt tgctggatgc gataatcgtc aaaacgtcga aaccaatgca gcacctgact
     5161 gacagcatat tcctgcattt gctcgcccat accggtatct tccaggcgaa aaagtggaac
     5221 agaagggttc agcatttcag ggtgtgcctg tagcttgctc aaaatagaat caacaccggc
     5281 cccgagtgcg aacaccgctt taagatcgcg ccctgccagc atttcaacag gaggatgcca
     5341 gactaaagca taatcagcag agtcattatc tccgcttttc catgctctga ctcttgcctg
     5401 aggaatagct ttgcgcagtg cctcaatcca ccattgggta tcgaacgttg ggtgataaaa
     5461 gatgatatcc atactgactc ccgaaaagcg ttgtgcgaaa tttattcgca cttatcgtta
     5521 tgatctacaa aggccaccag cataacaaat ccgtggtcgg tggcaaaaaa agcagatttc
     5581 gcttactaaa accacacatt gattgaagtt tgaataaacg cgcgattttt tcaaaaagtt
     5641 tgttgacctc aggtcatgat ttccctaaat tagcgcccgt tccagcaaga caggaacgac
     5701 aatttggtga ggtgtccgag tggctgaagg agcacgcctg gaaagtgtgt atacggcaac
     5761 gtatcggggg ttcgaatccc cccctcaccg ccagattata agcgcatgtt attgtttaac
     5821 atgcgctttt ttattttccc ttccagacag tatgccatcg ggtatgccat ctgaaaccgg
     5881 tttatatgcc ccgcgatcat atcttatcgg acaatttccg atctttgagc atactggcaa
     5941 agctatactg ccagcatatc aggatagtgt tttgcgataa tatcattcag tctgtcaacc
     6001 agttctggtt ttctgaacgt gatatgcgcg gagcccttct taaagtatct gatgctgaac
     6061 atctcatctt catagctctc tttaccctga acagactgaa tgtgatcatc cagacgaagg
     6121 gtgatgtttc cccggttgtc ggggattggt ttaccgctga acaggtgcaa cattctttcc
     6181 agatcggcga gtcggtcagc ctgttgtccg gtgataagat gaaatcccca ccggtcccat
     6241 ctcaccagat tgttgacgat aattttactg ccaaatttac aggggcaatt ggttttgtaa
     6301 ttccagttca gccctctgaa gacgttgatc acccctcgct caaacacctc atccttattc
     6361 tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatcg
     6421 tactccagtg accggtacca tgtatcacgg gcctgcgtat ccattaatga cagcatcccg
     6481 gaacgttgca tcaggtcacg ccagatttcg cgatcaagat tccgggtaat ggctttcatt
     6541 gctgtttcgg gtttctccat cagccagcag ccgcagcgaa aatcctgttt catggcccag
     6601 tccggggcag ttttacctcc gatgctttcc gtcagcaccg agatttcagc cagttgtcgg
     6661 ataagtgtgt cgatttgcaa cagtgtactc tttcgtacag ccagaatgtg gcgaatgctg
     6721 gttgagcaga taacgtccgt atgcccgttg accacttccg gacgggagat tgtgatatct
     6781 gacataatcg atattccttt ttgcagataa atgaaaacgc cagccctgtg caggctggcg
     6841 tcagaggaat gaagtctggt gagtgagctt caccggttct ggtgcagaag ctgcagatga
     6901 cgcagggtac ggacatcctc ccgggcctgt tcgatacagg gcaacagtgc tgctgaatcc
     6961 gttttatcct catcgttgtc aaagataatt cccgatccgc agtcgatatt gtcctgcagc
     7021 cacgcaatca gaatatccag cgctgtttcc gtggttaatg atttcatgtt gttcctggtt
     7081 accagtcgaa agtgggtaaa cccggcaggc attcagcgct ggcatccaga tgaatgagac
     7141 tgacgccata acgccggatg agtgtgacga ccagacgacg gaacgttttt gacagcccca
     7201 ggcgtttaag ccgcagaacc gggtatgacc acgcatcggt acgtaacaga tatccgctac
     7261 ctgtaaaatg aatccattct gattcaccga agtcactggt ctggtgtgac agcgagtaca
     7321 accaggcgtt gtccgtttcc gtgatatgtg cggtactgca gcgtatgccg gtaagggtag
     7381 caaatggtgg cgtgcattct gctgaggtct ggtcagattc atcaacaaca cgaagggagt
     7441 agccgttatc gcagaccatg ttaatcagtt cagcgagctc aataccatcg acgtcaacga
     7501 caatgcgccc catgttcagt gcctgtacgt taacgctgtc ggcttccagt gtcagggcca
     7561 gtttcatcat ttcgcctccg gatacttacc caaggtaatg ttatttaccg ttctgtaatt
     7621 gtcgcgggtc atcaggccgg tcgcccggcg agcccggagg atatcaatac tgtttattaa
     7681 ctgagagcgg ggacaggtgc tgaatcccgg ctggtcggta cgcaccagcg cgtatttttc
     7741 cacgagaaaa ttaactgcat cacacagtga aatgcctgcc tcaatatgct gctcaatcac
     7801 acgttcatcg gcaaacggtg tgtcattcag tgtgaggcca taatgctggt ccagcagtcg
     7861 gatgagcagt gtctgccaga tggtgacggg agacgggcag cgagatacct cccgtacatg
     7921 tgtgtcggat aatgttttca tcttaagttc tctgtaactg gtagtgctaa aggtgagagc
     7981 ttcggggtta tgaggtggtt gcgagtgctg gtgtcggata aacagccaga taaacgtaac
     8041 cacaggagcc gagggtgtcg gcttcgcagg tcagcccttt tgcattcagc gtgacggtat
     8101 gctgatatcg gggattcagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca
     8161 gcggaaaggc ctggtccaga tggtatgcat ctgcgtcgct gaagcggcct ctgataccgg
     8221 cgcggtcggc aaggtaatgc aggcggttac cctcttgcac cagacgagcg ccaaaacagg
     8281 gcctcactgt gcagggaagc ccccaccagg ggcggtcgtg attgtcatcg ggatgagtta
     8341 tcccggagag tttgtctgac acgataaaat cctctctgaa aagtggttaa gaatggttta
     8401 acgatgacag gtcagccggc gcgctgaccg gtttcggtga agatgtatgc attgatgaga
     8461 atggcctcat ccacagcggc gtctgaggtc atctggtcgt actcgtttcc cgggtgacgg
     8521 aacatcagtt cagggtgaca ctcatcagcc ggacatacgg gccaaaactg tccttacggc
     8581 gttctgcaaa cacggcaaga acgccgggaa tatcctgcac ttcacgacca gtatacgctt
     8641 cagcactgcc gtgccagcg
//
LOCUS       DAZFXT010000174         8599 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.174, whole genome shotgun sequence.
ACCESSION   DAZFXT010000174 DAZFXT010000000
VERSION     DAZFXT010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8599)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8599)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8599
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.174"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..138
                     /locus_tag="ACHKW0_004453"
     CDS             <1..138
                     /locus_tag="ACHKW0_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 family protein"
                     /protein_id="HFO7477156.1"
                     /translation="YRWHGSAEAYTGREVQDIPGVLAVFAERRKDSFGPYVRLMSVTL
                     N"
     gene            212..580
                     /gene="cbeA"
                     /locus_tag="ACHKW0_004454"
     CDS             212..580
                     /gene="cbeA"
                     /locus_tag="ACHKW0_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system cytoskeleton
                     bundling-enhancing antitoxin CbeA"
                     /protein_id="HFO7477157.1"
                     /translation="MSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLSSCGYVYLAVYPTPEMKN"
     gene            669..1042
                     /gene="cbtA"
                     /locus_tag="ACHKW0_004455"
                     /pseudo
     CDS             669..1042
                     /gene="cbtA"
                     /locus_tag="ACHKW0_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system
                     cytoskeleton-binding toxin CbtA"
     gene            1039..1233
                     /gene="yeeW"
                     /locus_tag="ACHKW0_004456"
     CDS             1039..1233
                     /gene="yeeW"
                     /locus_tag="ACHKW0_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7477158.1"
                     /translation="MMTLEADSVNVQALDMGHIVVDIDGVNITELINKAAEKGYSLRV
                     VDDRDSTETPATYASPHQLL"
     gene            1279..1359
                     /locus_tag="ACHKW0_004457"
                     /pseudo
     CDS             1279..1359
                     /locus_tag="ACHKW0_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1648..1794)
                     /locus_tag="ACHKW0_004458"
                     /pseudo
     CDS             complement(1648..1794)
                     /locus_tag="ACHKW0_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            1848..2030
                     /locus_tag="ACHKW0_004459"
     CDS             1848..2030
                     /locus_tag="ACHKW0_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006576 - biogenic amine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HFO7477159.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(2131..2460)
                     /gene="tmaR"
                     /locus_tag="ACHKW0_004460"
     CDS             complement(2131..2460)
                     /gene="tmaR"
                     /locus_tag="ACHKW0_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HFO7477160.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(2632..3690)
                     /gene="yeeA"
                     /locus_tag="ACHKW0_004461"
     CDS             complement(2632..3690)
                     /gene="yeeA"
                     /locus_tag="ACHKW0_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HFO7477161.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(3888..4361)
                     /gene="sbmC"
                     /locus_tag="ACHKW0_004462"
     CDS             complement(3888..4361)
                     /gene="sbmC"
                     /locus_tag="ACHKW0_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HFO7477162.1"
                     /translation="MNYEIKQEDKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAIYYDNPDETPAEKLRCGTVVTVPGYFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(4480..5646)
                     /gene="dacD"
                     /locus_tag="ACHKW0_004463"
     CDS             complement(4480..5646)
                     /gene="dacD"
                     /locus_tag="ACHKW0_004463"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HFO7477163.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQHRLIAVIMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            5855..7282
                     /gene="sbcB"
                     /locus_tag="ACHKW0_004464"
     CDS             5855..7282
                     /gene="sbcB"
                     /locus_tag="ACHKW0_004464"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000175 - 3'-5'-RNA exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008310 - single-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HFO7477164.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(7325..7552)
                     /gene="tsuB"
                     /locus_tag="ACHKW0_004465"
     CDS             complement(7325..7552)
                     /gene="tsuB"
                     /locus_tag="ACHKW0_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization sulfurtransferase
                     TsuB/YeeD"
                     /protein_id="HFO7477165.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAGMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(7566..8597)
                     /gene="tsuA"
                     /locus_tag="ACHKW0_004466"
     CDS             complement(7566..8597)
                     /gene="tsuA"
                     /locus_tag="ACHKW0_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001647169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate utilization transporter TsuA/YeeE"
                     /protein_id="HFO7477166.1"
                     /translation="MCGALLGFVMQRGRFCLTGGFRDMYLAKNNRMFYALLIAISVQS
                     VGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRAGEGLIGSWI
                     ALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVLLVITLWVVM
                     KELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLSEATGRMFGL
                     GITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRAADAQTTLRS
                     GLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASWLVYVRPQRK
                     ARLATAAAN"
BASE COUNT         2280 a         2234 c         2006 g         2079 t
ORIGIN      
        1 taccgctggc acggcagtgc tgaagcgtat accggtcgtg aagtgcagga tattcccggt
       61 gtgctggccg tgtttgctga acgccgtaag gacagttttg gtccgtatgt ccggctgatg
      121 agcgtcaccc tgaactgagt gggaattctg atgagcagaa ttatcgccac taccggacca
      181 ttcttagccg attttctgta aggattttat cgtgtcagac acactccccg ggacaacact
      241 tcccgacgac aatcacgacc gcccctggtg ggggctgccc tgcaccgtga cgccctgttt
      301 cggggcacgt ctggtgcagg agggtaaccg gttgcattac cttgccgacc gcgccggtat
      361 cagaggcctg ttcagcgatg cagatgcgta ccacctggac caggcctttc cgctgctgat
      421 gaaacaactg gaactcatgc tcaccagcgg tgaactgaat ccccgccatc agcataccgt
      481 cacgctgtat gcaaaagggc tgacctgcaa agccgatacc ctcagcagtt gtggttacgt
      541 ttatctggct gtttatccga cgcccgaaat gaaaaattaa ctctccagaa tagccttctg
      601 ctacggcctg gtgttttcac cacgccactt ttccattttt atatctgcat atcaggaaaa
      661 tcttcagtat gaaaacatta cctgtattac ccgggcaggc gaccagttct cacccgtctc
      721 ctgttgaaat ctggcagata ctgctgtccc gactgctgga ccagcactat ggcctcacac
      781 tgaatgacac accttttgcc gatgaacgtg tgattgagca gcatattgag gcaggcattt
      841 cactgtgtga tgcggtgaac tttctcgtgg aaaaatacgc gctggtgcta ccgaccagcc
      901 gggattcagc gcctgtaccc gctctcagtt aataaacagc atcgatatcc tccgggctcg
      961 cagggcgacc ggcctgatga cccgcgacaa ttacagaacg gtaaataaca ttaccctggg
     1021 taagtatccg gaggcgaaat gatgacactg gaagccgaca gcgttaacgt acaggcgctg
     1081 gatatggggc acattgtcgt tgacattgat ggtgttaata tcactgaact gattaataag
     1141 gccgctgaaa aaggttattc actccgcgtg gtggatgacc gtgactctac cgaaacaccg
     1201 gcaacttatg ccagccctca ccagttgctg taagacaatg cagtgatgca gggtatagtg
     1261 ccattaacca cagaaacagc actgttgtcc tgtattaagc aggcaagaga agatgtcctt
     1321 accctgcgcc atctgcagct tctgcaccag aaccggtgaa tctcactcaa caggcttcat
     1381 ttcccctgac gccagcctga atacagctgg cgttttcatt tataaacaga aaggaaaacc
     1441 gataataatg gaatggacgc cccgaccatg aagcaacggt aggatattac tgataaacct
     1501 tccagtggag gtgcgttatg gccgttttaa cgattagcat tgacctgaca aaaaatgaat
     1561 tccaaatcca cggtctgggt aggaacagaa aaatctaagc tcagaaaacg gattaaagta
     1621 agcgtcaacg gagcaccgta ttgacgctta tttattggtg agaactacgt tccatggcag
     1681 gagttcgtca acacggttgg agggccattc cggcagtatg ctcagaatat ggcgcagata
     1741 cgcttccgga tcgataccgt tcagacggca ggtgcctgct tttccggcaa caagcaggaa
     1801 tctcagcgct gaagcaggaa ttagaggtaa aaacgcccta ccgggccatg aatcacccgg
     1861 tcattggagt agtaaccaaa gcagatttag ccagcatgga acaaatatct ttggtgaaaa
     1921 gttggttacg ggaagctgga gcgcacaacg tgttagtaac cagtgcagtt aataacaatg
     1981 gagttacaga actttttgcc ttgctgcata cagaagaagg ctgttgttaa tttcgattgg
     2041 ttctcagatg agaatgatga cggtgataca cagataagaa tcaatgagca atgagggttg
     2101 ccgggcaacc ctcattgaat aaaacgggaa ttacttcgct tcgccgtttt tcatttcgcc
     2161 catagctttc agctttttgg agatatcgcg gcgttctttg gagagctcgg catttttgat
     2221 gatgtaatcg tcaacgcgat cttcatagtc acctttcatg ctggcgatga tgccctggat
     2281 tgcttcaacg ctcatccctg gcttgatgta atcgctcagg ttgtccagca acaggacgcg
     2341 cttctggttg tcacggatct ttttctcaac gtcctgaatt tcacgttgca gtttgttctt
     2401 acgacggaac agacgaacaa attccagtac gtcctggaat gaaggcttgg tagtttccat
     2461 ttttataccc ctgataatgt gagagtcgga ttcgtttaag caaccgctat tcgttagggc
     2521 caacattact catagctgcc gcagatgaca atgcttttat cccttttcac tatcataccc
     2581 tttatccttg ctgaatcgaa gcagcagcaa gatgattctg aagttcagga attatttgcg
     2641 caaggcccgg caaatcagat tcgatagcaa ctcaagctga tgtgccgttt ccatgttcag
     2701 ccacacataa ccatagattg gtgtttccac aaccttcagg tcatggtggt tattcagcaa
     2761 ctgacgcagc tcttccacag catcgttcaa tttttccgta ttggcaaaaa ccggctgcgg
     2821 attaccttcg tacagcgcat gaacaaggct cagcagtatt tgctgcatca tatgctgggt
     2881 atcacgaagt ttttgcgcgt tcaataacac gaaatggctg gggcgcgtgg cccagtacgc
     2941 attgatttgc aactccagca tacaaaccag attgcggtta atggtctgga taccttcata
     3001 tatcgatttt ggaatacggg tttctttgct ggcgggcgca atcagcccac gcattttcac
     3061 ggcatcggtc aagagttttt gtagatggct ttccagacgt gggcgttcga gtaagttcgg
     3121 tgagaatgca gattgataga cccgattata ctcggtcaga cttttcgcca gttgaatgcg
     3181 ccagtggatg aacgcccgtt gtggccagat accggtaaac aacattgcca gtaaagagcc
     3241 gaggatcaca tcgccgcttc gccataacgc cgtatcaatt tcacctgtcg gggaaccaac
     3301 aacaattgcc agcgtcaccc caatcaataa tccttgatac ggtttcttgc ccagcgccag
     3361 ccaaccgcaa agaaacatcg ccgccgcgca ccagactaac atcagcggta acgaaattaa
     3421 ctccagttgc agagcgataa gacccaaaat cgaacccaac accgtaccgc caatacgttc
     3481 gaaggcgcga gggacaacgt taccccagaa cgagattggc cccataatca ccaccatggt
     3541 gaccagcggc caggtgcttt ccgggatagt aaacaggcgg ataatgagaa aagtgagcag
     3601 gaatgccagc gcgacccgag taccatgcac aatgcggtaa tggcggtata cccggatttc
     3661 aaacgggctt aatgacttat cggcacgcac gcagaaactc cgtaatgaag attaaaagcg
     3721 ctcataactc atctgtcggc cagaaggcgc gcctctgaat tatccgacgg acataccggg
     3781 aacagtgtaa tttcccggca ggagcgaaaa atttgaaagg cgcatcttac tcttttcgct
     3841 ttcaaaaaaa gagtggtcat cgcgttaaca caccgccctg agatgaatta gtgatgtttt
     3901 ggctgcaccg caacatacat ttcgatatcc cagtacccat cttccgcgcc attgttcaag
     3961 taaacctcga agcagggctt tggtaacatt tcataagcac tgtcctgcaa gaggctatta
     4021 aagaactgat accagggttt agcaaaatca tcaccgacta cacgagctac cgccaccgca
     4081 tactgaccac ctgaaatttc tgtcagaatg acgccctcac tgttttcggg aagcgtaaag
     4141 taacccggca ccgtcacgac ggtgccgcag cgtaattttt cggcgggtgt ttcatctgga
     4201 ttgtcgtaat agatagcaac ccactccttc ggcacaatat ttttactatc tacccacatc
     4261 atcaactgct caaaaccttt ctttaccgtc tgttcccagg ggccaacgag atggaaacct
     4321 gcaacggtac gtttatcttc ctgcttaatc tcgtagttca tgacgcctcc attaatactg
     4381 tttttatata cagtatagtt gcaatattaa aaccacaagg aatgagtgtt gattatgcga
     4441 gcagactcgc actcctgcca gtctgctgca aaagaaaggt caggccttat ggtggaaata
     4501 atcactcagg cgagaaaaca tgctgccttc cccgacagat tccagggtaa ccagcggcca
     4561 gtgcgccacc tgtttatcac ggtcgtaaag ttcaatttcc cctacccgct gatgggcgct
     4621 aattggcgcg gtgagttctt taccatcaag ggtatatttg gctttgatat gtggaatttc
     4681 ggctttcggt agcaccatcc agaactcttg ttccgttccc aggtcgatat tttctttatc
     4741 gccataccag atgcgttccg ttccgacctt tttcccacgg tgcaaaattt gcaccgtagt
     4801 aaagttttgt tgcccccaac gcagtaattt tcttgcctct tcctcacgac cttttgcact
     4861 gtcagccccc attatcactg caatgagacg atgctgccca tctacagccg aagcaatgag
     4921 attaaacccg gcaccagaag tatgacccgt tttcaggccg tcaacattca tggttttatc
     4981 ccacaataat ccgttacggt tttgctgggt gataccgttc caggtgagac ttttctcact
     5041 gtacatatga taaaactcgg gctcgccgtg gatgatagca cgagaaagca cagctaaatc
     5101 ataagccgag ctatgctggc caggtgcatc cagaccatgc actgtttcaa aatgcgtatc
     5161 cttgagatgc agcttctcgg catagttgtt catcatttca acaaactgcc gttgcccacc
     5221 ggcaatatag tcagccagag caacacaagc gtcatttccg gaatccacaa ttaaaccacg
     5281 gcttaaatca cgtaccgata cgcgatcgcc ctctttcaaa aacatcagtg aagaaccgac
     5341 aaacaccgga ttatctttcg cccacgcatc gcgccccacg gtgacaatat cgtctggcgt
     5401 aatgcgatga ctatcgatag cgcgatccac gacataaccc gtcatcagct ttgtcaggct
     5461 ggcgggattg cgctgttgat gctcattacc cgcggtgagg atctgaccgg tggtgtaatc
     5521 catcaatacc caggacccgg catgaatctc tggaggctga ggtgaaaaag gaatgttttc
     5581 cgccgcaaaa ccagacgata agttaaaaac gaacaaagaa gcagcaataa taagacggcg
     5641 tttcaacagc aaaccctcag gagtttcaaa tagctgttct ttttacggaa atacttatga
     5701 actggctgga ataaagtgca agaaaatgtg actaccctct catttttatc tgacatgatc
     5761 tgttgccact cgctgccaaa ttgtggcgct aaagctgatt agcacggtga tatttgatac
     5821 tctggcagac agcagaaata acggatttaa cctaatgatg aatgacggta agcaacaatc
     5881 tacctttttg tttcacgatt acgaaacctt tggcacgcac cccgcgttag atcgccctgc
     5941 acagttcgca gccattcgca ccgatagcga attcaatgtc atcggcgaac ccgaagtctt
     6001 ttactgcaag cccgcggatg actatttacc ccagcctgga gcagtattaa ttaccggtat
     6061 taccccgcag gaagcacggg cgaaaggaga aaacgaagcc gcgtttgccg cccgtattca
     6121 ctcgcttttt accgtaccga agacctgtat tctgggctac aacaatgtgc gtttcgacga
     6181 cgaagtcaca cgcaacgttt tttatcgtaa tttctacgat ccttacgcct ggagctggca
     6241 gcatgataac tcgcgctggg atttgttgga tgttatgcgt gcctgttatg ccctgcgccc
     6301 ggaaggaata aactggcctg aaaatgatga cggtctaccg agctttcgcc ttgagcattt
     6361 aaccaaagcg aatggtattg aacatagcaa cgcccacgat gcgatggctg atgtgtacgc
     6421 cactattgcg atggcaaagc tggtaaaaac gcgtcagcca cgcctgtttg attatctctt
     6481 tacccatcgt aataaacaca aactgatggc gttgattgat gttccgcaga tgaaacccct
     6541 ggtgcacgtt tccggaatgt ttggagcatg gcgcggcaat accagctggg tggcaccgct
     6601 ggcgtggcat cctgaaaatc gcaatgccgt aattatggtg gatttggcag gagacatttc
     6661 gccattactg gaactggata gcgacacatt gcgcgagcgt ttatataccg caaaaaccga
     6721 tcttggcgat aacgccgccg ttccggttaa gctggtgcac atcaataaat gtccggtgct
     6781 ggcccaggcg aatacgctac gcccggaaga tgccgaccga ctgggaatta atcgtcagca
     6841 ttgcctcgat aacctgaaaa ttctgcgtga aaatccgcaa gtgcgtgaaa aagtggtggc
     6901 gatattcgcg gaagccgaac cgtttacgcc ttcagataac gtggatgcac agctttataa
     6961 cggctttttc agtgacgccg atcgcgcagc aatgaaaatt gtgctggaaa ccgagccgcg
     7021 taatttaccg gcactggata tcacttttgt tgataaacga attgaaaaac tgttgttcaa
     7081 ttatcgggca cgcaacttcc cggggacgct ggattatgcc gagcagcaac gctggctgga
     7141 gcaccgccgc caggtcttca cgcccgagtt tttacaaggt tatgcagatg aattgcagat
     7201 gctggcacaa caatatgccg ataacaaaga aaaagtggcg ctgttaaaag cgctttggca
     7261 gtacgcggaa gagatcgtct agtttgaagc ctccgccgct gatacggcgg aggacaatgt
     7321 tgggtcaggc tttttgaacg gtgatgctcc acgcagcatc gccaatctgc tgataatcgg
     7381 tgatggcatg tccttcttct gccgcccatt gtgggattgc ttctgttgcc tgggtgcaat
     7441 caaactcaat taccaactca tcgccgctgg ccattcctgc cagtgctgct tttgcttcta
     7501 tgagtggaaa cggacagacc tgcgtcacca catccagctt tttaatcgcc atctttacac
     7561 cttacttaat ttgccgcagc agttgccagt cgcgccttac gctgcggtcg aacatacaca
     7621 agccaggacg cagtccagac tccgagaatc ataaatacaa ggccaatcca gccctgccag
     7681 gtcatcattg cagtcataac cagtccatta ccgatagagc aaccacctgc aatactggca
     7741 ccgaagccca tcagtacacc acctaacccg ctacgtaatg ttgtttgtgc atcagctgcg
     7801 cgaacgcgga actcacggct cgctttggct gcaataaatg accccacgaa gatccctaac
     7861 accaggaaaa cgccccagtt aatgtatttc acgtcacccg cgaccagaaa ttgcagaata
     7921 ttggccgttg gggaagtgat tcctaaccca aacatgcgtc cggttgcttc actcaggggc
     7981 caggctaaaa gcgcaatcaa accgatgagt acagcagtga caaagggatg ccagcgtttt
     8041 tcaaacagaa tatgagcgat cccggttcgg cgcggcggta aggtcgcgac tttgagtttt
     8101 ggcttcttca actctttcat caccacccag agcgttatca ccagcaaaac ggcaaccaac
     8161 ggccacacag acaaattaaa agtctcagca atagagttat gttcagtact gtagtgctgc
     8221 aaggtttgat ttaaaccact ggcatgtgga gaacgcatca ccgcactcat caccatataa
     8281 gtgaaaagcg cgatccaact gccgatcaat ccctcacccg cgcgatacca ggtcccggtg
     8341 gcacatccac ccgccagaac aattcccagc ccaaagatat acccacctat aacagtacca
     8401 agccacggga acgccccggc ttcgtaagtc agtaggcccg cctgaattaa cgcaaaaacc
     8461 cccacacttt gtaccgaaat tgcaatcagc aaggcgtaaa acatgcgatt atttttcgcg
     8521 agatacatat cgcgaaaacc acctgtcagg caaaaacgcc cacgctgcat gacaaatccc
     8581 agcagagcac cacaaatta
//
LOCUS       DAZFXT010000175         8597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.175, whole genome shotgun sequence.
ACCESSION   DAZFXT010000175 DAZFXT010000000
VERSION     DAZFXT010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.175"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..556
                     /gene="ydeJ"
                     /locus_tag="ACHKW0_004467"
     CDS             38..556
                     /gene="ydeJ"
                     /locus_tag="ACHKW0_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HFO7477167.1"
                     /translation="MNINRDKIVQLVDTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(600..2645)
                     /gene="dcp"
                     /locus_tag="ACHKW0_004468"
     CDS             complement(600..2645)
                     /gene="dcp"
                     /locus_tag="ACHKW0_004468"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HFO7477168.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPKIMPMLQHRGLNI"
     gene            2782..3528
                     /gene="ydfG"
                     /locus_tag="ACHKW0_004469"
     CDS             2782..3528
                     /gene="ydfG"
                     /locus_tag="ACHKW0_004469"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HFO7477169.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            3617..4303
                     /gene="rspR"
                     /locus_tag="ACHKW0_004470"
                     /gene_synonym="ydfH"
     CDS             3617..4303
                     /gene="rspR"
                     /locus_tag="ACHKW0_004470"
                     /gene_synonym="ydfH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator rspR"
                     /protein_id="HFO7477170.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            4481..4684
                     /gene="ydfZ"
                     /locus_tag="ACHKW0_004471"
     CDS             4481..4684
                     /gene="ydfZ"
                     /locus_tag="ACHKW0_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HFO7477171.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(4720..6180)
                     /gene="ydfI"
                     /locus_tag="ACHKW0_004472"
                     /pseudo
     CDS             complement(4720..6180)
                     /gene="ydfI"
                     /locus_tag="ACHKW0_004472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
     gene            complement(6269..7552)
                     /gene="ydfJ"
                     /locus_tag="ACHKW0_004473"
                     /pseudo
     CDS             complement(6269..7552)
                     /gene="ydfJ"
                     /locus_tag="ACHKW0_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            7684..7926
                     /locus_tag="ACHKW0_004474"
     CDS             7684..7926
                     /locus_tag="ACHKW0_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7477172.1"
                     /translation="MPHRYPAAKINKMPKGSVPALQQEMLRRVSKRYDDAEVIIKSTS
                     NNGLSVTRTADKDSAKTFVQETLKDTRESADEWFVR"
     gene            complement(8035..8192)
                     /locus_tag="ACHKW0_004475"
                     /pseudo
     CDS             complement(8035..8192)
                     /locus_tag="ACHKW0_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701877.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
BASE COUNT         2293 a         2170 c         1938 g         2196 t
ORIGIN      
        1 aaatcacctt tacatataaa agcgtggaca aaaaacagtg aacattaata gagataaaat
       61 tgtacaactt gtagataccg atactattga aaacctgaca tccgcgttga gtcaaagact
      121 tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta agttggctag
      181 cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg ttactttcac
      241 cgatcaggca aagatgaaaa tcctcagcgt aagccagcaa tctcttgaac gatattctgc
      301 ggtgagtgag aaagtggcag cagaaatggc aaccggtgcc atagagcgtg cggatgctga
      361 tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg gtacgccagc
      421 gggtaccgtc tggtttgcgt ggcatattaa aggccagaac tacactgcgg ttatgcattt
      481 tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg cccagctgct
      541 gcaattactg ctataaccag gctggcctgg cgatatctca ggccagccat tggtggtgtt
      601 tatatgttca agccacgatg ttgcagcatc ggcataatct taggtgcctt accgcgccat
      661 tgtcgataca ggcgttccag atcttcgctg ttacctctgg aaaggatcgc ctcgcgaaaa
      721 cgctgcccat tttcacgcgt taatccgccc tgctcaacaa accactggta accatcatcg
      781 gccaacattt gcgtccacag ataagcgtaa taacctgcag catatccgcc accaaaaata
      841 tgggcgaaat aactgctgcg atagcgtggc ggtatagcag gaagatccat attttccgcc
      901 accagcgccc gcaattcaaa atcatcgaca tcctgcattg cttcgttttc ttccaggcaa
      961 tgccagcgca tatcgagaag tgcggcgcta agcagttcgc tcatctcata ccctttgttg
     1021 aacaggctgg cattacgcat tttctgttgc agttcgtcag gcattgctgc cccgctctga
     1081 taatgccggg cgtagcgagc gaatacctgc ggatgcgttg cccagtgttc gttgatttgc
     1141 gacggaaatt cgacaaaatc acgcggcgtg ttggtgccgg aaagcgtggc ataacgctgg
     1201 cgggcaaaaa ggccgtgcag cgtatgacca aattcatgga ataaggttat tacatcatcc
     1261 cagagtaaca acgcaggctc accggcagcg ggtttctgat aattgcaaac gttataaatt
     1321 accggatgcg tttcattaag cgttgattgc tcaacaaaat tgcccatcca tgcaccgccg
     1381 ctttttgaat cacgggcgaa gaaatcaccg taaaataacg ccagtcccac gccattatga
     1441 tcaaaaattt cccacacacg aacgtcagga tggtagacag gaatatcaaa acgttcgaca
     1501 aacttaatac cgaagagctg attcgcggtc cagaatacac cttcattcaa caccgtgttt
     1561 aattcaaaat atggcttgag ctgcgcctca tcaagatcaa atttctcccg ccgtacctgt
     1621 tcggcataaa atgcccagtc ccacggctgc gcgctaaacc cgccttgctg cttatcgata
     1681 accgcctgta tggaggctaa ttcatcgcta gcacgttgac gcgccgctgg aacaatttcc
     1741 cgcataaagt tgagtgctgc ttcaggtgtt tttgccatct gatcggcgat tttccatgcg
     1801 gcataatgag gaaaaccaag cagtttcgcc tgctgtgcgc gaatctctac cagacgttga
     1861 atgatagcgc gggtatcatt gccatcattt ttttccgctc gcgtccagcc cgcaataaac
     1921 agtttttcac gcgtcgcacg atcgcgcatt tcggcaagcg ccggttgttg ggtggtattc
     1981 agcagcggaa tcagccattt gttatccaga cctttctcgc gagccgccac tgccgccagc
     2041 gcaatctctt gctcactcat tcctgccagc tgcgcgatat cgttcacaac cagaccgccg
     2101 gatttatttg ctgcaagtaa ccgctggtta aactggctgg tcagggtcgc agcttctgta
     2161 ttcagtactt ttaattttgc tttatcagct tgcgcaagtt tggctccggc aaggacaaaa
     2221 cgttgatgaa taacctccac caggcggatg gattcactat caagccccag ggattcacgg
     2281 cgctgccaga cagcatctac ccgcgcgaat aattcaccgt tcagatagat atcattagcc
     2341 agttccgcca gttcagcgga aaactgttca tcaagacgct gtaattcatc attggtatgc
     2401 gccgcagtca tcgcaaaaaa gacgctggta acgcgggtaa gtaattctcc gctttgttcc
     2461 agtgccagaa tagtattgtt gaaatcaggc gtttgcgggt taagcgcgat ggcagcaatt
     2521 tctgcccgct tttgctgcat tccctcatcg aatgccgggc gatagtgatg attggcaatt
     2581 tgatcaaaat gaggagccag atacggcagt gtgctttgca caaggaaagg attcattgtt
     2641 gtcattttct tctcctgaac gcgaggtgtt ccatagcgta ggcttactga taacgtagtg
     2701 caatcttgca aaccagtatt acccgctctt aagcatcacg tgctatgtta ttgacacaca
     2761 aaagcgttga ggaacagtga gatgatcgtt ttagtaactg gagcaacggc aggttttggt
     2821 gaatgcatta ctcgtcgttt tattcaacaa gggcataaag ttatcgccac tggccgtcgc
     2881 caggagcggt tgcaggagtt aaaagacgaa ctgggagata atctgtatat cgcccaactg
     2941 gacgttcgca accgcgccgc tattgaagag atgctggcat cgcttcctgc cgagtggtgc
     3001 aatattgata tcctggtaaa taatgccggc ttggcgttgg gcatggagcc tgcgcataaa
     3061 gccagcgttg aagactggga aacgatgatt gataccaaca acaaaggcct ggtatatatg
     3121 acgcgcgccg tcttaccggg tatggttgaa cgtaatcatg gtcatattat taacattggc
     3181 tcaacggcag gtagctggcc gtatgccggt ggtaacgttt acggtgcgac gaaagcgttt
     3241 gttcgtcagt ttagcctgaa tctgcgtacg gatctgcatg gtacggcggt gcgcgtcacc
     3301 gacatcgaac cgggtctggt gggtggcacc gagttttcca atgtccgctt taaaggcgat
     3361 gacggtaaag cggaaaaaac ctatcaaaat accgttgcat tgacgccaga agatgtcagc
     3421 gaagccgtct ggtgggtgtc aacgctgcct gctcacgtca atatcaatac cctggaaatg
     3481 atgccggtta cccaaagcta tgccggactg aatgtccacc gtcagtaatt tttatacccg
     3541 gcgtaactgc cgggttattg cttgtcacaa aaaagtggta gactcatgca gttaactcac
     3601 tcacaagcaa gaacgaatga ccgtcgaaac gcaacttaat cccacacagc ctgtcaatca
     3661 gcagatttat cgtattcttc gtcgcgacat tgtccattgc ctgattgcac caggcacacc
     3721 gttgtcggaa aaagaagttt ctgttcgttt caatgtgtca cgccagccgg ttcgtgaagc
     3781 ctttattaaa ctggcggaaa acggcctgat tcaaattcgt ccgcagcgtg gcagctacgt
     3841 caacaaaatt tccatggccc aggtgcgaaa cggcagtttt atccgtcagg ccattgagtg
     3901 cgcggtggcg cgtcgggcgg cgagcatgat taccgaaagc cagtgctacc aactggaaca
     3961 aaatcttcac cagcaacgca ttgccattga gcgcaagcaa ctggatgatt tttttgaact
     4021 tgatgacaac ttccatcaac tcctgacgca gattgccgac tgtcaactgg cgtgggatac
     4081 cattgagaac ctgaaagcga ccgttgatcg cgtgcgctat atgagtttcg accatgtttc
     4141 tccaccagaa atgctgttac gccagcatct tgatattttc tctgccctgc aaaaacgtga
     4201 tggcgatgcg gtagaacgtg caatgacgca acatttgcag gaaatcagcg aatccgtgcg
     4261 ccagatccgc caggaaaaca gcgactggtt tagcgaagag taattcattt actctcatcc
     4321 catccgggat gagaggcttt tccccccgcc ttacggctca tgcatgcatc aaaaaagatg
     4381 tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat tgcgcccgtt
     4441 acgtgggctt cgactgtaaa tcagaaagga gaaaacacct atgacgacct acgatcgtaa
     4501 ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc acactggcaa
     4561 gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg gaaaaaccgt
     4621 agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc tcggcatgaa
     4681 ctaagtgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg aatatttcgc
     4741 cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca cttttgcagt
     4801 aaacaagcta tcgtctggca aatcatcacc aaagattgcc ttaatcgcca gcaatgactg
     4861 gacgcgcgct ttcccttcgg cactactttg tacagccttc tgaataacag gtaacagtgg
     4921 gtcactgatt tctatcggat ttccctgttc atcaacacca ccgacataac gcatccaacc
     4981 cgcgacgccc agcgccagca gatcgaactt gctgtcatgc gccagatgcc agcgaacaga
     5041 atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc aggttcgatg
     5101 acgtaacgcc gggttgctat agcgtgcaat taatcggtta gcgtaatcct gcaaatcaac
     5161 gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg ccgcatgacg
     5221 ataatgttca tcttccatgc agtcattaat gtgctgatat ccggcaagat accccagata
     5281 cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat aaggcagcac
     5341 atcgctaacc agttcggctc ccgctttttc ccattccgga cgtccggcaa caaagttatc
     5401 ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc gcacaccggt
     5461 aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac ggtccaccat
     5521 tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat caacggcttg
     5581 cgcgtaggaa gtgacaacat cacgcataac atgaccgttt tctggcatgt tgtcacatga
     5641 catgacggta aatgcgggaa gtcctgccgc tttacggcga gccagcgcct caacaatcac
     5701 ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca tcgggtgatc
     5761 gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga ttgtcagaga
     5821 gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc catccatctg
     5881 tacgtgcaag gcttttttaa cgacgccaac gacgcgagcc gtccacgcat cggccgacat
     5941 ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct gttcgccgcc
     6001 gattaagttg acctaataat atccccagtc actgaaatgt tctgtagcaa gaatatcggc
     6061 atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaacaa ttcttggggc
     6121 caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat tatttcccat
     6181 aacaattcct taaatttaaa tatagcaagc tacatgtttt gttatatgaa taaaaatccc
     6241 ctctccggta agagaaggga ttgaggattt acagacttct ggaaggttgc gcagctctta
     6301 caacacgcgg ttgatcttcc gcagcgtctt ccagcgcact taaatcacgg tctttcacct
     6361 ctggcatttt cagcgcagag attaaaccaa tcactgaata tgccatgatc ataatggcga
     6421 tcggatacca ggattccgtc atggtgcaga aaatacccgc caggatagga ccaaaaccgg
     6481 aagcgataag accaccaatt tctttagaaa tagccatccg ggtaaagcgg tttttacagc
     6541 cgaacatttc tgccatggta atgttttcca gagcaaataa tcccagcacc gcacagttat
     6601 gaatcacaat cagtgcaacc ataatggtgc tcggggcata gcttttatct acaatgatag
     6661 aaagcattgg ccatgccagc acaatcgcgg aggtattcat aataatatac gggatccggc
     6721 gaccaatttt atcggataac caaccaagga acggaatggt cataaagccg agaatcgaac
     6781 taatcatcaa tgcatctgtt ggaattgctt tgttaaacaa taacgtctgc actaaatagc
     6841 ctgcaaggaa agtctgaatt aaacctgagt taccagcctg accaaaacgc agccctgttg
     6901 ccagccagaa ggatttgctc tggaacatgc taccagcagg tgcaggtttt gctgtcggtt
     6961 ggttgctgtc attaaccttc tcaaagaccg ggctttcttt cagattcata cgcaaccaga
     7021 tagcaaagac catcacgaca acgctcgcca ggaacggtat acgccatccc cacgccagca
     7081 gttcctcttt actgagaatg aagaacataa aggcccagat tgccgttgcg ctcaaggttc
     7141 cgcagttagt tcccatagct acaaatgagg agataattcc gcgcttacct tttggcgcat
     7201 attccgccag catcgtaccg gcaccggaaa tttccgcacc tgcacccacc ccctgaataa
     7261 tacgcagcgt caccagcaag atgggtgcaa aaacaccaat ctgtgcatag gtcggtaaca
     7321 caccaattaa ggtggtacag atccccatca tggtgatggt aataaagagc acttttttac
     7381 gccctattct gtcgcccatt ttgccgaaaa taaatgctcc gacaatacgc gccacataac
     7441 ctgcaccgta ggttcccatt gccagaatta acgccattgc cgttgatgat tcaggaaaaa
     7501 atatttcatg aaacactaac gctgcgccga gcgaatataa ctggaaatcc ataattcata
     7561 gatgtttttt actattctgt gggtttttgg gtgttttcta agttttttca gatggttgta
     7621 ttttttctaa aaatccctaa tctcgatttt gctgtttatt tgaggccttt ttatgtccca
     7681 tatatgcccc acagataccc cgcagccaaa atcaacaaaa tgccaaaagg ttctgttcct
     7741 gccctgcaac aagaaatgct gcgacgtgtc agtaaacgtt atgacgatgc agaagtgatc
     7801 atcaaatcca ccagcaacaa tggcctttca gttactcgca ccgccgataa agattctgca
     7861 aaaacttttg ttcaggagac gctgaaagat acccgggagt ctgctgacga gtggtttgtt
     7921 cgctaattaa cgagtaaaat cagtaacggc tggaaatcat tcaatactca cactatctaa
     7981 aagttcacca gccaacagcg gtaagttctt tcatacgacg tgccgcggat cctattattc
     8041 tagtatgaaa tccctaaccg atttcaaatt ctccagactg ctcagattct cttttatccc
     8101 acgctggcgc tgataaatct cgtcattgca atcgacctgc gctatcgctg ccgcagttct
     8161 tccagtttct gcatcgataa ctccatcttc tgagcaataa acctcaccta tgcaatatct
     8221 tatgtcaatt atttcaattg cattaatatc ttgaatggat tacatagagt taatgtatcc
     8281 agtactccct attctcttgc atataataca tgttgcaact tacatctcag cgctatgaaa
     8341 aaacaccacc tctctcttta tgaaattctg gatttaccaa gcgctaattt atcatttcaa
     8401 tctaccttta agtattgcat ttatctccct acgaggtcat actttaggaa gttaaatatg
     8461 aatgataata tacctacagc gcgaaatcac aaacaatcga cttgtattac agaaaaaaac
     8521 atgcctatat ttttaaactt cacagcaggc agtatcttac ctgagaatga gctagcatct
     8581 ttacgttata ttgtgca
//
LOCUS       DAZFXT010000176         8511 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.176, whole genome shotgun sequence.
ACCESSION   DAZFXT010000176 DAZFXT010000000
VERSION     DAZFXT010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.176"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..359
                     /locus_tag="ACHKW0_004476"
     CDS             <1..359
                     /locus_tag="ACHKW0_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477173.1"
                     /translation="KSYDFFGDVVLSQCINGSLIFALEYGSPYIKGCLVTGWENGFKG
                     KNSPEGFCFAERNIPEAVWFGKNNILIIIRNQMDVGTWKGKYIIYDNAKKSGERAYSS
                     DNLPSKDGYMIFALNK"
     gene            437..1651
                     /locus_tag="ACHKW0_004477"
     CDS             437..1651
                     /locus_tag="ACHKW0_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477174.1"
                     /translation="MPVRLNTIPDAESYPAPPARIKWLSALLLMLSVGVAITSLFASD
                     ELAKNGPHFWGLACGVPAFIWSLVASVRWLFFITQYIRADAWNKRREEVILQETRRGR
                     RALQILSFSVQTALNGDSVAETTTAFLARQQVLNTYADLRGEDTVRRSVIPVLTNKPV
                     TGRLSEIISRLFIDIRPQLFLLPPDFHINVLLEIDAPLSGASVRTIWQDEWQKAGLPE
                     ARLFSAPEPGLAAVDDWLDNFIQEKAVLLVISVYLEPKTPERTAESSTALLLANRLTQ
                     TALTPLALLHRPERITDTEMMASGIAQALDWMPVQPDAISGIWTAELDREQRTALLSL
                     NQPFTQEALMYELDAFLGRSGPAAPWLSVAAATLAAIQSQHPQLTLSGVQGGHYSWAT
                     VVSPFVSPQEAS"
     gene            1708..5034
                     /locus_tag="ACHKW0_004478"
     CDS             1708..5034
                     /locus_tag="ACHKW0_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImcF-related family protein"
                     /protein_id="HFO7477175.1"
                     /translation="MTVAVVVALCTFIHRKPALLGVEPGSSQQMYWMAGVIAAGVVFL
                     IIMGVYHLLNRHAAVQNFELRRQLAAGDDYRAVTTKKADSRHPAMVELAAFLRDRYGP
                     FWRRNVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPSAEPDVTLLTALKK
                     LRRSRPLDGIIWALTEEQSRQTAQLDKGWRELINGGKRLGFQAPLYLWQVCDNGDYQA
                     GRPLQSVGCLLPERCTPEQLTAMLEAHTLPLTEQGMSQLLADNRHDFLLRLAHTLAER
                     GIAHWQSVLKPLLAGGAFSSLRLRGLMFSPPLTAVPEAAPHAWLPSQVWAGVTGDNAR
                     GRAVGFPWLRAALMSAVCVLAIWGAGMTTSFFANCALVQETGIQTARALDTRLPLAEQ
                     LVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVD
                     HLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADSLRY
                     ENIPEGVQQSVFPSLLTFWMANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTL
                     YQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVT
                     ARHEEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFTGSWLAFLNSIRWKKEDSLS
                     GILDQLTLMADARQSPLIALTDTLAWQAAAGRENRGLSDSLAKSAQELFNGKEKTPQQ
                     SREGDVPVGPLDKTFAPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDP
                     QEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLT
                     PAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINQFIAANL
                     SGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELQAKPA
                     RDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGS
                     WSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQ
                     VFLTGRSMKDAEEYGEDADE"
     gene            5027..6637
                     /gene="tssA"
                     /locus_tag="ACHKW0_004479"
     CDS             5027..6637
                     /gene="tssA"
                     /locus_tag="ACHKW0_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HFO7477176.1"
                     /translation="MSELLQKLTRSCFVDRDALDVARTQAALWQTWLLPVTANTPVGE
                     DPGYHDDFLRIRDEMNKLSGADTDLICQLAESLLLTQAKDVRIATYYIWARLHRDGER
                     GLAEGLALLTGLVERFGTQLLPSRPASRKMALEWLAGEKMLDSLARYPEVAKEDFANI
                     VAALSQLTVSFTAWPEDQHSPSLMLLINALESRLAQSGGMNAVVPQNSSSVPAPSSPV
                     DAPQVQTITSGRDLLDQAKVLARYLNEQPQGWLSAHRLMKTLRWDTVHELPPDVDGKT
                     RLAPPRTESRNQLKRLYAQQNWTELLEQADLMFSTGVSHFWLDIQWYLHQALTKAGAP
                     WDRWTAVIRQDLALLLERLPGLENLAWNDDTPFADEVTRNWIAQQVMMREDGAWLAGK
                     AAVPTDDATNDVLALEPEALEMADSQGVEAALGWIQTRPGITTARQRLLLRLLMARVA
                     EQYGKNEMALLLLEELDTAAQGLTLTQWEPDLLFEVKARQLKLLRLRAHRYADKALLN
                     RKMEILLGTLVTIDPVRAAVLCDTQHKD"
     gene            6643..8061
                     /locus_tag="ACHKW0_004480"
     CDS             6643..8061
                     /locus_tag="ACHKW0_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001402492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HFO7477177.1"
                     /translation="MGTGYFLVRGDKTTCGGKIIGGADDHTIMGIPQARDMDRVTCGR
                     YPGMFIIVGGVPETDIHGRLMAGTLDSQSSCPCKARFIASMMDDTYETDDGGSEAEQH
                     AQSAKKDLTSGSDSSSDDVKLDYRIKLSGNKILTPLNIPDYKEMISGGSTKNTEKIDF
                     TITNKGDEAEGLSLEVLDGNEVIYSEQQTGKYFDKGEHAWQWDGYSNQGILDTTKLKS
                     KSLLVRLIALCGDMMIKVDYPLHNSPEEQKWVDVKVDRKQKTVDIIWRLAVSDGGIKG
                     SNPKLSPVPYNDLVNLTKNGVEFYWSRNGSRGGGIGENIVTAIGVFKVNVKAEINITP
                     SMRTFSLISSLDPDFQASVSLSGFEKIYYNYGDSYKDIQDELQALLDANNRYKWDSAH
                     EMGHKVLDEYGEGSSPDYSWTHKGTSTLMQKTIPGNVMPAQGEIDVMKYGKYRPDMYT
                     RLVAADEDVQGLIWLSRIKFDD"
     gene            8054..8476
                     /locus_tag="ACHKW0_004481"
     CDS             8054..8476
                     /locus_tag="ACHKW0_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477178.1"
                     /translation="MTRVVIALLFLAGCTSQMKYSNVVRIRNVCSSDINVFIPEYVFG
                     GNVIKQHHIYLPVNGIDVLSYFYTENINDGFQYEEAFTVNKDSSGNFDYPFVILSSGR
                     EKKFGKNDLIELSRDISTKDDKKHGVVKYMIDSQDICP"
BASE COUNT         2061 a         2168 c         2434 g         1848 t
ORIGIN      
        1 ctaaaagtta tgattttttt ggagatgtgg ttctttctca gtgcataaat ggttctctta
       61 tctttgcgct ggaatatggt tctccatata taaaaggttg tctggtaact ggttgggaaa
      121 atgggtttaa aggtaagaac tctcctgaag gcttttgttt cgcagagaga aatattcccg
      181 aagcggtatg gtttggaaaa aacaatattt taattatcat ccgaaaccaa atggatgttg
      241 ggacatggaa agggaaatat attatctatg ataatgcgaa aaagtcagga gaaagagcat
      301 actcatcaga taatctacca tcaaaagatg gttacatgat ttttgcatta aataaataaa
      361 atgactctat agataacttt aaatttcact tggttattaa cttctgtccg ataatgcaaa
      421 ataaagggat gtactgatgc ctgtccgact taataccatt cccgacgcag aatcctatcc
      481 cgcaccgcca gcccgcatta aatggctgtc agccttatta ctgatgctgt ctgttggtgt
      541 ggcaataacc tcattattcg ccagcgatga actggcaaaa aacggcccgc atttctgggg
      601 gctggcctgc ggtgtgcctg cctttatctg gtcgctggtt gcttcagttc gctggttgtt
      661 ttttatcacg cagtacattc gggctgatgc ctggaataag cgccgggaag aggtgatttt
      721 gcaggaaacg cgacggggac gacgggcgtt gcagatactt agtttttccg tacaaaccgc
      781 gctgaatggt gattccgttg cagaaactac aacagcattt ctggcgcgtc agcaggtgtt
      841 gaatacttac gctgatttgc gcggagaaga tactgtgcgt cgcagtgtca ttcccgtatt
      901 aaccaataaa ccggttaccg ggcgtttatc cgaaattatt tcccgccttt ttattgatat
      961 cagaccgcaa ttgtttctgc tgccccctga ttttcatatc aacgtactac tggaaattga
     1021 tgcgccgctt tcaggggcct ccgtgagaac tatctggcag gacgaatggc aaaaagccgg
     1081 actcccggaa gcccgtctgt tttctgcccc ggagccgggg ctggccgccg tggacgactg
     1141 gctggacaac tttattcagg aaaaggccgt actgctggtg atttccgtgt atctggaacc
     1201 gaagactccg gaacggacgg cagaatcctc caccgcgctg ttgctggcaa accggttgac
     1261 gcaaaccgcg ctgacgccgc tggccctgct gcatcgtcct gaacgtatca cagatacaga
     1321 gatgatggcg tcaggcattg cacaggcgct ggactggatg ccggtgcagc cagatgccat
     1381 atccggcata tggactgccg aactggacag ggaacagcgt accgcgttgt tatcccttaa
     1441 ccagccgttt actcaggaag ccctgatgta cgagctggat gcctttctgg ggcgctccgg
     1501 accggcagcg ccgtggctgt ccgtcgctgc ggcaacgctg gcggcaatac aatcacaaca
     1561 cccccaacta acgctcagcg gcgttcaggg cgggcattat tcatgggcga ccgtggtgtc
     1621 gccattcgtt tccccacagg aggcatcata gtggttccgg gattaaaccg tctccggccc
     1681 cgttctgttt cagggcctgc catcgtcatg accgtggctg ttgtggttgc gctttgcacg
     1741 tttattcacc gtaaaccggc attgctgggc gttgaaccgg gtagctcaca gcaaatgtac
     1801 tggatggctg gcgtgattgc cgcaggcgtg gtctttttga ttatcatggg ggtttatcat
     1861 ctgctgaacc gccacgctgc cgtacaaaac tttgaactcc gcagacaact tgccgccgga
     1921 gatgactatc gtgcggtgac gacaaagaaa gccgacagcc gtcatccggc aatggtggaa
     1981 ctcgccgcct tcctgcgcga ccgctacggt ccgttctggc ggcgtaacgt ccgcctgctg
     2041 ctggtgaccg gcgagcctga acaggcagaa gccatcgcgc cggggctgac cgggcaacac
     2101 tggctggagg gcgaccacac ggtgctgata tatggcggca ggccatcagc ggagcctgat
     2161 gtcacactgc tgaccgcctt aaaaaaactg cgccgcagcc gtccgctgga cggcatcatc
     2221 tgggcgctga cagaagaaca gagccgccag acagcgcaac tcgacaaagg ctggcgcgaa
     2281 ctgataaacg gcggtaagcg actcggtttt caggccccac tctatttgtg gcaggtctgt
     2341 gacaacggtg attatcaggc cggacgcccc ctgcaaagcg tcggctgcct gctgccggaa
     2401 cgctgcaccc cggaacaact gaccgccatg ctggaagctc acaccctgcc gctgacggaa
     2461 cagggcatgt cgcaactgct ggctgataac cgccatgatt ttctgctgcg tctggcccat
     2521 acccttgcag agcggggtat cgctcactgg caaagcgtcc tgaaaccgct gctggcaggc
     2581 ggcgcattct cttccctgcg cctgcgtggt ctgatgttca gcccgccgct taccgccgta
     2641 ccggaggccg cacctcatgc gtggctgccg tcacaggtct gggcgggcgt gaccggcgat
     2701 aacgcacggg ggcgtgcagt gggctttccg tggctacgcg ccgcgctgat gtccgctgtc
     2761 tgcgtgctgg caatatgggg ggccggaatg acgacctctt tcttcgccaa ctgcgctctt
     2821 gttcaggaaa ccggtatcca gacggcgcgg gcgctcgata cccgcctgcc gctggcagaa
     2881 caactggtgg cgctgcatac cctgcagggc gaactggaac gcctgcaata tcgtatccgc
     2941 gaaggtgcgc cgtggtatca gcgttttggc cttgaacgta accaacaact gctcgccgcc
     3001 gcctttcccg gctatgcgca ggcggcaaac cggctggtgc gcgacgtggc cgttgaccat
     3061 ctgcaacagc aactgaacgc ctttgtcgcc ctgccgccca acagtcctca gcgtaccgcc
     3121 accggtgaac aacgctataa gcagcttaag gcattgctga tgacttcccg cccggaaaag
     3181 gccgacgctg cctttttcag taccacgctg atggcagaca gcctgcgcta cgagaatatc
     3241 ccggaaggtg tgcagcagag cgtgtttccg tcactgctga ccttctggat ggcgaacctg
     3301 ccggaacacc cgcagtggaa aacctcgccg ccaccggaac tgaccggcgc ggtgcgtaaa
     3361 atcctgctgc gccagattgg tgtgcgtaat gccgaaaaca ctctctacca gaacgtactg
     3421 caacaggtgt cccgcaacta cgccgatatg acgctggcgg acatgaccgg ggataccctc
     3481 accgaatctc ttttcagtac ggaacagacg gtgccgggga tgttcacccg tcaggcgtgg
     3541 gaaggacagg tcagggaagc catcgagcag gtggtgacgg cgcggcacga ggaaatcgac
     3601 tgggtgctca gcgaccgcca gcaggatacc tctgcggata tatcgccgga tacgctgcgt
     3661 aaccgtctca cctcacgcta ctttaccgac ttcaccggaa gctggctggc gtttctcaac
     3721 agcattcgct ggaaaaagga agactcgctc tccggcattc tcgaccagct gacactgatg
     3781 gccgatgccc gtcagtcgcc gctgattgcg ctgaccgata ccctcgcctg gcaggcggcg
     3841 gcaggcaggg aaaaccgtgg cctgtcagac tcgctggcga aatcggcaca ggaactgttt
     3901 aacggcaagg agaaaacgcc gcagcaatcc cgtgaaggcg acgtgcctgt cgggccgctg
     3961 gataaaacct tcgctccgct gctgcggttg ctgggcgata aagccggagg cggcgacagc
     4021 cagctgagtc tgcagaccta cctcacccgc gttacccgtg tgcgtctcaa actgcaacag
     4081 gtgaccaacg cccccgaccc gcaggaaatg acgcaacagc tggcacagac ggtactgcag
     4141 ggcaaaaccg ttgacctcac cgacacccgc gactacggac ggttaatcgc cgccagtctg
     4201 ggcgaggaat ggagtggctt cggtcaggcg ctgttcgtcc gcccggtgga gcagtcgtgg
     4261 cggcaggtgc tgacgcctgc ggcggacagc ctgaaccgcc agtggcagcg ggcgattgtc
     4321 agccactgga atcaggactt cgctggtcgc tacccgttca aagcctcaca gaacgatgcc
     4381 tccctccccc tgctggcgca gtatctgcgc gatgacgggc gcatcaacca gtttattgcc
     4441 gccaaccttt ccggcgttct gaaacgtgaa ggccgctact gggtggcgga cgccatgaac
     4501 acgcaggggc tgacggtcaa cccggacttt atccgcgccc tgaaccgcct gcgcgacgtg
     4561 gctgacaccg cctttgccag cggcgatgcc ggaatacatt ttgaactgca ggcaaaaccg
     4621 gcccgagacg tgatgaagac acatctggtg attgacgggc aggaactgga gtatttcaac
     4681 cagaaagaac gctggcagcg tttcaactgg ccggatgaac agtggcaacc cggcgcatcg
     4741 ctgagctgga ccagtacgca ggcgatggaa cgcatactgg cggattaccg ggggagctgg
     4801 agccttattc gcctgctgga acaggcgcag gtgacaccgg tggacagcag cacctttaaa
     4861 gtggtgtgga aagcgcagga cggcctgccg ctgaattacc tgctgcgggt tgaacagggt
     4921 aaagggccgc tggcgctgct ggaactgaaa aacttccgcc tgccgggaca ggtatttctg
     4981 accggcagga gtatgaaaga tgcggaagaa tacggggaag acgccgatga gtgaactgtt
     5041 gcaaaagctg acccgtagct gttttgtcga ccgggatgca ctggatgtgg cgcgaacaca
     5101 ggcagcgctg tggcagacat ggctgctgcc ggtgacggca aatacgccgg tcggggaaga
     5161 ccccggctac cacgacgatt tcctgcgtat ccgcgacgag atgaacaaac tttccggcgc
     5221 ggatacggac ctcatctgcc agcttgccga atccctgctg ctgacgcagg caaaagacgt
     5281 gcgcatcgcc acttactaca tctgggcgcg actgcaccgc gacggtgagc gcggactggc
     5341 tgaggggctg gcgctactga ccggactggt ggaacgcttc ggtacgcaac tgctgccctc
     5401 gcgaccggcc agccggaaaa tggcgctgga gtggctggca ggggaaaaga tgctcgacag
     5461 tcttgcccgc tacccggaag tggcaaaaga ggattttgcg aacattgttg ctgccctcag
     5521 tcagttgacg gtttccttca ctgcatggcc cgaagaccag cattccccct ccctgatgct
     5581 gctgattaac gcgctggaat cacgtctggc gcagtcgggc gggatgaacg cggtggtgcc
     5641 gcaaaacagc agcagcgttc ctgcaccgtc atccccggtg gatgcgccac aggtacagac
     5701 catcacctcc gggcgtgacc tgctcgacca ggcgaaagtg ctggcccgtt atctgaacga
     5761 acagccgcag ggctggcttt cggcgcaccg gctgatgaaa accctgcgct gggacacagt
     5821 gcatgaactg ccgccggatg tggacggcaa aacccggctg gccccgccgc gcaccgagtc
     5881 acgcaaccag cttaagcggc tgtatgcgca gcagaactgg acggaactgc tggagcaggc
     5941 cgacctgatg ttcagcaccg gcgtcagcca tttctggctg gatatccagt ggtatctgca
     6001 tcaggcgctg actaaggcgg gcgcaccgtg ggacaggtgg acagccgtta tccgtcagga
     6061 cctggcgctg ctgctggaac gtctgccagg gctggaaaat ctggcatgga acgacgacac
     6121 accgttcgcc gacgaggtga cgcgcaactg gatagcgcag caggtcatga tgcgggaaga
     6181 cggcgcatgg cttgcaggga aagccgctgt accgacagac gacgccacaa atgacgtact
     6241 ggcgctggag ccggaagcac tggagatggc agacagtcag ggcgtggaag cggctctggg
     6301 ctggatacag acccgtccgg gcatcaccac ggcacgccag cgcctgttat tgcgactgct
     6361 gatggcgcgg gtggcggaac agtacggtaa aaacgaaatg gcgctcctgc tgctggaaga
     6421 actggatacc gccgcacagg ggctgacact gacgcagtgg gagccggacc tgctgtttga
     6481 ggtaaaagcc cgacaactga aactgctgcg cctgcgggcg caccgctatg cggataaagc
     6541 cctgctgaac cgaaaaatgg aaatcctgct gggaacgctg gtgacaatcg atccggtacg
     6601 ggcagcggta ttgtgtgata cgcaacataa ggactgatgg caatgggaac aggctacttt
     6661 ctggtcaggg gggacaaaac cacctgcggc gggaagatta ttggaggcgc ggacgaccat
     6721 acgatcatgg gtatcccaca ggcacgggat atggaccggg tcacctgcgg cagatatccg
     6781 ggaatgttta ttattgtggg cggcgttcct gaaacagata ttcacggcag gctgatggcg
     6841 gggacgctgg acagccagag cagttgcccc tgtaaggcac ggtttattgc gtcgatgatg
     6901 gatgatacgt atgagacgga tgacggggga agtgaagcgg aacaacatgc gcagtcggcg
     6961 aaaaaggatc tgacttctgg ttctgattca tcctctgatg atgtaaaact ggattacaga
     7021 attaaattat caggtaataa gatactgacg ccactgaata tacctgatta taaagaaatg
     7081 ataagcggtg gtagtacgaa gaatacagaa aaaatagatt ttactatcac aaataaaggc
     7141 gatgaggctg aaggactttc tctggaagtg cttgatggta atgaagtgat ttacagtgaa
     7201 cagcagaccg gtaaatattt tgataaggga gagcatgcct ggcaatggga tggctacagt
     7261 aatcaaggca tactggatac aactaagcta aaaagcaaat cactactcgt aagactgatt
     7321 gctttatgtg gtgatatgat gataaaagtt gattatccac ttcataattc accagaggaa
     7381 caaaaatggg tcgatgtaaa ggtagacaga aaacaaaaaa cagtagatat aatatggcga
     7441 ctggcagtta gtgatggagg gataaaagga agtaatccta agttatcccc agttccttat
     7501 aacgacttgg ttaacctgac caaaaatgga gttgaatttt attggtctcg aaatggaagt
     7561 cgcggcgggg ggattggtga aaatattgtt acagcgattg gtgtttttaa agtaaatgta
     7621 aaggctgaga taaatattac tccatccatg cgaacttttt ctttgatatc atcattagat
     7681 cctgactttc aggcatccgt tagtctatca ggatttgaaa aaatatatta taactacggt
     7741 gatagttata aggatattca agatgaatta caggcgcttc ttgacgctaa taacagatat
     7801 aaatgggata gtgctcatga aatggggcat aaagtactcg atgaatatgg agagggttct
     7861 agccctgatt attcgtggac tcataaagga acatctactc ttatgcaaaa aacaatacca
     7921 gggaatgtta tgcccgctca gggagagatt gatgtaatga aatatggaaa atatcgtcct
     7981 gacatgtata ctcgattggt tgcagcagat gaagatgttc agggattgat ctggttaagc
     8041 aggataaaat ttgatgacta gagtagttat tgctttatta tttcttgccg gatgtacttc
     8101 gcaaatgaag tattccaatg tcgtcaggat taggaatgta tgttcctcag atattaatgt
     8161 gtttattcct gagtatgttt ttggaggtaa tgttattaag caacatcata tttacttacc
     8221 tgttaatggg attgatgttt tgtcttattt ttatactgaa aacatcaatg atggtttcca
     8281 atatgaagaa gcattcaccg taaataaaga tagctcgggt aattttgatt atccgtttgt
     8341 tatcttatca tcaggacgtg aaaaaaaatt tgggaaaaac gatctaattg aattatccag
     8401 ggacatttca acaaaagacg ataagaagca tggggttgta aagtacatga tagatagtca
     8461 ggatatttgc ccatagtatg attcatatca taatcaactg aaaaaatgac a
//
LOCUS       DAZFXT010000177         8430 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.177, whole genome shotgun sequence.
ACCESSION   DAZFXT010000177 DAZFXT010000000
VERSION     DAZFXT010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.177"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            36..1609
                     /locus_tag="ACHKW0_004482"
                     /pseudo
     CDS             36..1609
                     /locus_tag="ACHKW0_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            complement(1578..3314)
                     /gene="aceK"
                     /locus_tag="ACHKW0_004483"
     CDS             complement(1578..3314)
                     /gene="aceK"
                     /locus_tag="ACHKW0_004483"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HFO7477179.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3595..4899)
                     /gene="aceA"
                     /locus_tag="ACHKW0_004484"
     CDS             complement(3595..4899)
                     /gene="aceA"
                     /locus_tag="ACHKW0_004484"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HFO7477180.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(4929..6530)
                     /gene="aceB"
                     /locus_tag="ACHKW0_004485"
     CDS             complement(4929..6530)
                     /gene="aceB"
                     /locus_tag="ACHKW0_004485"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HFO7477181.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLASCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6799..7728)
                     /gene="metA"
                     /locus_tag="ACHKW0_004486"
     CDS             complement(6799..7728)
                     /gene="metA"
                     /locus_tag="ACHKW0_004486"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008899 - homoserine O-succinyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0019281 - L-methionine biosynthetic process
                     from homoserine via O-succinyl-L-homoserine and
                     cystathionine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HFO7477182.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7885..8328
                     /locus_tag="ACHKW0_004487"
     CDS             7885..8328
                     /locus_tag="ACHKW0_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HFO7477183.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         2181 a         2086 c         2066 g         2097 t
ORIGIN      
        1 aaattcaaga catcaataaa caatgagata tttaaatgat tactcgtatc cctcgtagtt
       61 ctttctctgc aaatattaat aatacagccc agacaaatga acaccaaacc ctgagtgaat
      121 tgttttataa agaactcgag gataaatttt ctggcaaaga gctggcgacg cctctattaa
      181 aaagcttctc agagaactgt agacaaaatg gtcgtcatat ctttagtaac aaggattttg
      241 tcattaaatt ttccacgtcc gtcttacaag ctgataagaa agaaattacg ataattaata
      301 aaaacgaaaa cacgacactt actcaaacca ttgccccaat atttgaagaa tacctaatgg
      361 aaattttacc tcaacgctca gacactcttg ataaacaaga attaaaccta aaatcagata
      421 gaaaagaaaa agaattccca agaattaaac ttaatggtca atgttatttt ccggggcgac
      481 cccaaaaccg tatagtatgc cgacacattg ctgcacaata tattaatgat atttatcaga
      541 atgttgatta caaaccccat caagatgatt actcttcagc tgaaaaattt ctcacgcact
      601 tcaacaaaaa atgcaaaaac cagactttgg cgttggtttc cagccgtcct gaggggcgtt
      661 gcgttgctgc ctgcggtgat ttcgggctag ttatgaaagc atattttgac aagatggaat
      721 caaatggcat cagtgttatg gcagccatat tactggtgga taaccatgct ttgacggtcc
      781 ggctaagaat aaagaacaca actgaaggat gtacccatta cgtggtttcg gtttatgatc
      841 ctaatgtaac taacgataaa ataagaatta tgagcgaaag caaagaggat attaaacact
      901 attctctgat ggattttatg aatgtagatt atagcctcct gaaatggtca aatgatcatg
      961 ttattaacca atctgttgca ataattccag cacttccgaa agaacagcta ttgatgttaa
     1021 aaggatctgt ggatgaaata acccctccat tatcaccagc aacgatgaat ttgctaatgg
     1081 caattggtca gaatcaccaa cttacgcaac tgatgattca actccagaaa atgccagaac
     1141 tacatagaac agaaatgttg actgcctata atagtggaca tatgaacgtt attaatacta
     1201 tttttaacgc attacccact ctttttaata cgtttaaatt cgataaaaaa atatgaagcc
     1261 cctcctcctg gcaaataatt ctaatgaata tcccggtttg ttttcagcga tacagcataa
     1321 acaacaaaac gttgtagaga cggtttatct tgctttatct gaccatgcac gcctgtttgg
     1381 atttaccgct gaagatatta tggatttttg gcaacacaaa gcgccacaaa aatactctgc
     1441 ctttgagttg gcttttgaat ttggtcaccg ggttattgct gaattaatcc ttaatacatt
     1501 aaataagatg gctgaaagct ttggctttac ggataaccct cgatacattg cggagaaaaa
     1561 ttatatggaa gctttactca aaaaagcatc tccccatacc gtacgctaaa tctttgcctg
     1621 cgccgatacg cataaacatc ttccacatgc ccttcacgta tgcggttttg tagtgcgcgc
     1681 cagtaatcag cgcggaacag gtcggcgtgc atctcttcaa acagcggacc aatacgcggg
     1741 tcggcgcata gccagtggcg aaactcttcc gggaaaacat cgcccggcga gacgctgtac
     1801 cacggttcgc tggcaagttc gtcttccgga tagcgcggcg gcgggatgtc gcggaaattc
     1861 acttccgtca tgtagcaaat ttcatcgtaa tcataaaaaa ccacacgccc gtgacgggtg
     1921 acaccgaagt ttttaaacag catgtcgcca ggaaaaatgt tagcagcggc aagctggcga
     1981 atagcgttac cgtattcttc aatggcgtcg cgcaactgct gaccttccac ttgttccagc
     2041 cagatattga gcggcaccat ccgccgctcg atatagagat ggcgaattac aatctgctca
     2101 ccaagatcgg tgattttttc cgctgcttcc tggagcagta attccattaa tgccggggaa
     2161 atatgccgct tctccagcac aaagttttca aactcctggg tgtccgccat tcggcccacg
     2221 cgatcgtgct ctttcaccag ttgatagcag gcacgaacgt gagcggcaga catctctttc
     2281 tgcggcgcga acttgtcttt gatgactttg aatacccgat caaagcccgg cagcgtaaac
     2341 accaacatca ccattccacg aatacccggc gcttcaatga actgctcatt acagccctgt
     2401 agataaacga gatattcgcg gtagctttcg gttttggcgt gcttctggca gccgatagcc
     2461 atatacaatt cagcagtggt tttacctggc agaatttccc gtagccactc gaccagcgct
     2521 gcgggcagcg gtgcataaac cataaaataa gaacgcgcaa agccaaaaac aatgctcgct
     2581 tcggcggtcg tcgtcaggca ggtatcaata aataactcgc cgtcgtccgt ctggtggatc
     2641 ggcagtaaga agggcaacgt gccggaaggc gtgatcagtt tgcccaccag ccaggcggct
     2701 ttattgcggt aaaacagttc gttcgccacc tgtaaatgac tttccgcgag gttgtctgtc
     2761 cccagcgttt ccgtcagatg gcgaatgatg taatgaatgt cacggctttt attctgccag
     2821 cgcaggcgca gcggcaggtc gctgataacg cgcatcagta gagattccca gccgtgatcg
     2881 gggtgaaagt ctttcgccag cgggcgggga atggtacgaa agcggcgctc tggctgagag
     2941 ctaaagataa aaagccgctc gggagtaagc gagcggtggt caaataaccg acagtacacg
     3001 gagttaaaaa agctctccgc aatctcgaag cgcgggtaat ccggcaacag ccgcgtgtaa
     3061 tgctctttaa cgcgcagtaa aaatgccgcg tccgtacttt ggccgttagt aatgcagcgc
     3121 agttgctcca cgaccagacc aacgtgatga tcgtaaagat ggatacggtt tttcatcgcc
     3181 tgctggacag catgccagtc ggcctgttcg aaacgctgct gcgccccgga agtcacttcg
     3241 aggaatcgac catactgagc gtcgaagcct tgcaaaatgg tttgagcaat cagtaattcc
     3301 aggccacgcg gcatttagcg ccctcatcag gagcagagaa ttgccggatg cggcgtaaac
     3361 gccttatcca gcctacgttc ggcaacggtt gtaggcatga taagacgcgc aagcgtcgca
     3421 tcaggcatcg tgcaccgatt gccggatgcg gcgtaaatgc cttatccggc ctacgttcgg
     3481 caacggctgt aggcatgata agacgcgcaa gcgtcgcatc aggcatcgtg caccgattgc
     3541 cggatgcggc gtgaacgcct tatccggcct acagtcagca acggttgttg ttgcttagaa
     3601 ctgcgattct tcagtggagc cggtcagcgc ggtgactgaa gacgtgccgc cctgaataat
     3661 agtcgtcact ttatcgaagt aacctgtacc cacttcctgc tggtgagata cgaaggtata
     3721 gccatctttc gcggcggcaa attccggctg ctgcactttc tcaacgtagt gcttcatacc
     3781 ctcgccctgg gcataggcgt ttgccaggtc aaacatgttg aaccacatgc tgtggatacc
     3841 tgccagggtg atgaactgga acttgtagcc catatccgac agctgctgct ggaagctggc
     3901 aatagttttg tcgtcgaggt ttttctgcca gttgaacgac ggcgagcagt tataagccag
     3961 cagtttgccc ggatatttcg cgtggatagc ttgtgcaaag cgacgcgcca gttccagatc
     4021 cggcgtggag gtttcacacc agaccaggtc agcatatggc gcatacgcca ggccacggct
     4081 gatcgcttgc tcaatgcccg catgagtacg gaagaagcct tcactggtac gctcgccagt
     4141 aataaattca ctgtcatacg ggtcgcaatc ggaggtaatc agatccgccg catcggcatc
     4201 ggtacgagca accagcaggg ttggaacgcc cgtcacgtca gctgcaagac gcgccgcgac
     4261 cagtttctga atagcttcct gagttggcac caaaactttg ccgcccatgt gaccgcattt
     4321 cttcactgac gccagctgat cttcgaagtg aactgccgct gcaccggctt caatcatcgc
     4381 tttcatcagt tcaaaggcat tcaggacacc gccaaaaccg gcttccgcat cggcaacgat
     4441 cggcaggaag taatcgacat agcgcggatc gcccggctca atgcctgcgg accattggat
     4501 ctgatcggca cgacggaagg tgttgttgat ccgctccacc acagctggca ccgagtttgc
     4561 cggatagagc gactgatccg gatacatgct ggccgccagg ttagcgtccg ccgctacctg
     4621 ccatcccgac agatagactg cttcaatacc cgctttcgcc tgttgcagcg cctgaccgcc
     4681 agtcagtgca ccgaggctgt tgatgtagcc ttttttcgac tcgccatgca gcagacgcca
     4741 cattttcgcc gcgcccagtt gcgccagggt gcattcagga ttgactgaac cgcgtaattt
     4801 caccacatct tccgcgctgt atgggcgagt aatgccttcc caacgcggtt gagtccactc
     4861 tttctgtaat tcttcaattt gttgtgtacg ggttttcatg tgcagatgct ccatagttat
     4921 gtggtggttt acgctaacag gcggtagcct ggcagggtca ggaaatcaat taactcatcg
     4981 gaagtggtga tctgttccat caagcgtgcg gcatcgtcaa aacgcccctg ggagaaacgt
     5041 tcttcgccca gttcgctggc aatgactttc atctcttcac ccagcatctg gcggaacaag
     5101 gctttggtca ccggtttgcc attgctcaac gttttttgat gatggatcca ctgccagatt
     5161 gaggtacggg aaatttcagc cgtcgccgca tcttccatca ggccataaat cggcacgcag
     5221 ccgttgccgg agatccatgc ttcgatgtac tgcactgcca cgcgaatgtt ggcgcgcata
     5281 ccttcttcgg tgcgttcacc atcacaagat gccagcagct gatcggcagt aatcggcacg
     5341 tcttgttcgc gcatcacttc aagctgattt ttacgggagc cgagaatgtc gttgaatacc
     5401 gccattgccg tatccgcaag gcctgggtga gcaatccatg tgccatcgtg accgttattg
     5461 gcttccagcg ctttatccgc ttttactttg ttgagcacct ggttattgcg ctcttcatct
     5521 ttgctcggaa taaacgctgc catgccgccc atcgcaaaag caccgcgttt atggcaggtt
     5581 ttaatcaaca ggcgtgagta agcattcagg aatggtttat ccatcgtcac tgcctgtctg
     5641 tctggcagga cgcgatcggg atagtttttc aacgttttga tatagctgaa gatgtaatcc
     5701 caacgaccgc agttcagacc aacaatatgg tcacgcagcg cgtgaaggat ttcatccatc
     5761 tggaacacgg cgggcagcgt ttcaatcagc aacgtcgcct tgatggtgcc gcgcggcaga
     5821 ttaaagcgat cttctgcata gctgaagact tcgctccacc aggccgcttc ctgccaggac
     5881 tgggttttcg gcagatagaa atagggaccg ctgccctttg ccaacagtgc ctgatagttg
     5941 tggaagaaat agagcgcaaa atcaaacagg ctaccgggga ttgcctcacc acgccaggtg
     6001 acatgttttt ccggcaagtg cagaccgcgt acccgacaaa tcaaaaccgc tggattgggc
     6061 ttgagctgat aaattttgcc tgcttcattg gtatagctga tggtgccgtt aaccgcatcg
     6121 cgcaggttaa tttgcccgtc gatcactttg ttccagtctg gtgccagtga atcttcgaaa
     6181 tcggccataa agactttcac attggcgttc agtgcgttga tcaccatctt gcgctcaacc
     6241 gggccagtta tctcgactcg acgatcttgt aagtccgcgg gaataccacg aattttccag
     6301 tccgtatcgc gaatggaagc tgtttccgaa ataaaatcag gcaaagttcc gttatcaata
     6361 tcttgctgct gctgaatgcg cgctgccaga agtttattgc gttgtggcgt aaaatgcgtc
     6421 accagctcag tcagaaattc taccgcttcg gcagtaagaa tttgcttctc ctgctcgcca
     6481 tacggcctta tgaaagccag ttcatcggtt gttgttgcct gttcagtcat cgtgcagctc
     6541 ctcgtcatgg atccgaaaac ttccccactg tgaacgaagg atcgttgtgc gcttttcgtt
     6601 cagcacaact aagactactc atttaaaatg caaaatcaaa aacaatttcc attttaatta
     6661 aaaatacttg ataacttatt gataacaata tgaataaaat ttattcacct cctgaggtgc
     6721 gtttcggaaa taaaaaaggc acccgaaggt gcctgaggta aggtgctgaa tcgcttaacg
     6781 atcgactatc acagaagatt aatccagcgt tggattcatg tgccgtagat cgtatggcgt
     6841 gatctgatag acgtaatagt tgagccagtt ggtaaacagc aaattaccgt gactacgcca
     6901 gctcgctcgc ggtgtatttt gcggatcatt gtgcgggaaa tagttatacg gtacttccgg
     6961 gcctagtccg gcttccacat cgcggaaata ttcctgcgcc agcgtttgcg catcatattc
     7021 gggatggccc gtcacaaagg caatgcgctt atctttgctg gcaaacagat atgcatcccc
     7081 ttcttccgtc tctgccagaa tttccagatc ggtgtaatca cgaatcaacg ctgccggaaa
     7141 gtcagcatag cgcgaatgtg gtgccaggaa tgaatcatca aagccacgcg tcagaagcgc
     7201 atgaggatgg agaatatgat gctcgtaaac gccagagagt ttgtcggtgc gagtttgctt
     7261 aggaatgccg tagaggatat tgagcgcggc ctgtaccgcc cagcagacaa acagcgtcga
     7321 ggtgacgtga tctttcgacc actccagcac ctgtttgatc tgcggccagt aagcgacatc
     7381 attaaactcc accaggccca gcggcgcacc ggttacaatc agaccgtcaa agttctgttc
     7441 ctgaatatct tcaaagttac agtagaagtt gttcagatgc tctgcgggcg tgttgcgcga
     7501 ttcacgggaa tcgatgcgca acagctgaat atcgacctgc aaaggtgagt ttgaaagcag
     7561 gcgcagaaac tgattttcag tttcaatctt cttcggcatc aggttaagga tcagaacctt
     7621 aagtggacga atttcctgac cagacgcacg agaagttgtc atcacaaaga cgttttcttc
     7681 acgcaagaaa ttgacggcgg gtagctcgtc cggcacacga ataggcataa cctgattacc
     7741 tcactacata cgcttatacg tttagacatc cagatagctg aagataacca gaaaatttgc
     7801 caatgtcgag cctgcatgtt gaaggtgaga aagtttcaag gaaaaggcta cgttagaata
     7861 taagaatgac gaaaaggaga gaaaatggtt attagtattc gccgctcaca gcatgaggaa
     7921 ggggaggaac tcgttgcgat ttggtgtcga tctgtcgatg ccacccacga ttttttatca
     7981 gcagagtatc gggccgagct ggaagagctg gttcgttcct ttctgccgga agcaccgttg
     8041 tgggtcgcgg ttaatgaacg ggatcagccg gttgggttta tgttgttaag tgggcagcat
     8101 atggatgcgc tgtttatcga tcctgatgtg cgcggctgcg gcgtgggtcg gatgctggtg
     8161 gagcatgcgc tctcgatggc accggaactg acaaccaacg ttaatgagca aaatgagcag
     8221 gcggttgggt tctataagaa ggtgggtttt aaggttacgg gacgctctga ggtggacgat
     8281 ttggggaaac cgtatccgtt gctgaatctg gcgtatgtgg gggaataagc cgcccctgcc
     8341 gtgcctgccc aggctggaga agacgcgcca gcgtcgcatc cgaccttttt ctgcaccagc
     8401 gcaaaaaggc ccagtcttcc gactgggcct
//
LOCUS       DAZFXT010000178         8405 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.178, whole genome shotgun sequence.
ACCESSION   DAZFXT010000178 DAZFXT010000000
VERSION     DAZFXT010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8405
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.178"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1086..1292
                     /locus_tag="ACHKW0_004488"
     CDS             1086..1292
                     /locus_tag="ACHKW0_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020234041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7477184.1"
                     /translation="MATPVSLMDDQMVDMAFITQLTGLTDKWFDKLIKDGGFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1386..1988
                     /locus_tag="ACHKW0_004489"
     CDS             1386..1988
                     /locus_tag="ACHKW0_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477185.1"
                     /translation="MKQQYQTRYELLHENYQKWLTGFTRHAVSWGVCHPNIYYFHNLT
                     PGWVSFNGEKPEIAIVPQSLHRLIYGPDKRSSPSLDDDLVVNLCTSEHLLVHHPMLEG
                     ILLSECERLKQHSLANKLISLFRQFGGTELRLKLVWLCWLDLMTGNSLDDWTKNLKHK
                     SEKDLEQWIIARQGQSEPLTNLMDQYVLMAYRTSVDAAHS"
     gene            2818..3115
                     /locus_tag="ACHKW0_004490"
                     /pseudo
     CDS             2818..3115
                     /locus_tag="ACHKW0_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            4383..5534
                     /locus_tag="ACHKW0_004491"
     CDS             4383..5534
                     /locus_tag="ACHKW0_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477186.1"
                     /translation="MGKELDDICTSCPYIDAVKRHKQQLGAIEEYTQWLKKEPRASYF
                     FLFQLYTRIHNTFFSQKQQLPFTPGGTHCPEPDVTLRDLTLSPGYHSDYAPQPIPEMD
                     SSAVVPPTNENTSPPEDTPDNTPAGGNTGQAEKTRNAGLTPIPEKRSGMPPEHLRFAT
                     GFPSQPKIAGPREKPMRTVHPDKIYREIIWFCSSYLLKSGPEATRTIINSVFSEWASI
                     NNDYPSPFSWVDSRDSEQCDWLWNAMQVRCVGTPLNPLTPEQKYWFACATFDNWEGWN
                     EQQVQFLLESNPRRNRAKFTQVSFQAPRIQHKAILLDELKSAREQQKRRDERADGSVP
                     LKLSGKIHKQLESIARSRGVLPKKLLNEMIEQAYHDLVATRQNSQIDSR"
     gene            5618..6489
                     /locus_tag="ACHKW0_004492"
                     /pseudo
     CDS             5618..6489
                     /locus_tag="ACHKW0_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
     gene            6707..>8405
                     /locus_tag="ACHKW0_004493"
     CDS             6707..>8405
                     /locus_tag="ACHKW0_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO7477187.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVEEPDVLSDSKNENRLL
                     FDKQKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQTLQQTGDTRELL
                     DILTDDGWLKNEYHGEEEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVEF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEVMMRDQLQKASAVLAVMDYTQMNSKADE
                     DVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGTLPVDEAWVDDFIRGAFGCMKKEYVCKDSELATEGATDL
                     WEGSLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENISEYLLLRHQGLQQSIQSL
                     QSHITSLLADIREIADCQEQMTTDVRMAMEEIDTKTRELLTGVCTSLEEELNDYFRSG
                     KRKEQQMLEEEDAEQRRSQSGLWGKISQWSGINNQGREDYRKRDFAPDSPEIKFSDRR
                     "
BASE COUNT         2238 a         2159 c         2100 g         1908 t
ORIGIN      
        1 tatattaagc gtaaccgaca ggttctcctt cataccatat taaatttttc caggtattgc
       61 cgctgcctta tttcgtccca tacaggaaaa ctcgtctggg tttctgacaa atacgcgaaa
      121 ctcacagccc ctcaatatca accacacgct gtaagccggt aatacctgaa aatcagccta
      181 ttaccgcccg cgcctccgtt accgaacgct caggtgacca gactgaagcc cagcacgcac
      241 tcgtaaaatt tgcggtcagt tccgtcagtc agtgggcaac cgcagagtta cgggcttcca
      301 ggtccacagg agtgagtcta taacgcgtgt gggaaatcat tttttctggc gttgacagaa
      361 cgccttacgg gctgctactt acccggtgtc ccgcaataca gccttagtgt tccgttatcg
      421 gggatccgga cgcaccgcaa caaccgggga agtatttctg gtcagagctg tgtcaagagg
      481 gatttctcag cccctcactc accggctcag tctcagggca gaagcattaa tacactcagt
      541 tcatgtctta tacaggaatg ggcatagggg gagctggcgt gtcactgctg attttacgca
      601 tcagcgttca tgtatctgca ctctgataca gaatgcttca cagcgctctg ttaccggaga
      661 gatttactct cttaggttat tctggcaaat cctttacgtt actctctgat gacttcttat
      721 atttataata ccaatcagac aaccttatcc catcggtgat atcagtctcc tgctcgccag
      781 gaacgggctg actcactgta accacctccg aaaacaactc tgcaattatc agaacgcctg
      841 cttctctccc tgtcctcacg aaaactatcc cctctttatc gcgcgtgcgt gcggaagcat
      901 cttttcgcaa cacccccctc cgggattccg ctacggctct gccatcgcaa tccccccgtt
      961 tatctccgga cagccgcatt cccgattatt ttttacgttt ctccccggtt gttatgccgg
     1021 tgaaggtggc gcgctgtttt catgaccaca ccggctggtt taacaacatc cggaggaaca
     1081 ttctcatggc taccccagtt tcgctgatgg atgaccagat ggtcgacatg gcgtttatca
     1141 ctcaactgac cggcctaacc gataagtggt ttgacaaact catcaaagat gggggctttc
     1201 ctgcgcccat caaaatgggg cgcagctccc gctggctgaa aagtgaagtg gaagcctggc
     1261 tgcaggcgcg tattgcacag tcccgtccgt aatttttaac cactaaccct tcgcccacag
     1321 cagacgcaat ctccggcctg ccggcggcgc tctgctgcct gttatccccc gtgaggaata
     1381 tgaaaatgaa acaacagtat cagacccgtt acgagctgct ccatgaaaac taccagaaat
     1441 ggctgaccgg cttcacccgg cacgccgtat catggggcgt gtgtcatccg aatatctact
     1501 atttccataa tctgacgccc gggtgggtgt cattcaatgg cgaaaaaccg gagattgcca
     1561 tcgttcccca gagtctgcac cggctgattt atggtccgga caaacggagc tccccgtccc
     1621 tggatgatga tctggtggtg aatttatgca ccagcgaaca cctgctggtt catcatccga
     1681 tgctggaagg gatcctgctg tctgaatgtg agcgcctgaa gcaacattct ctggcaaaca
     1741 aactgatcag cctcttccgt cagtttggcg gcacggagtt acgtctcaaa ctggtctggc
     1801 tctgctggct tgacttaatg accggaaaca gccttgacga ctggacgaaa aacctgaaac
     1861 acaaatcaga aaaagatctg gagcaatgga tcattgcacg tcagggtcag agtgaaccac
     1921 tgacaaatct gatggatcag tacgtgctta tggcataccg tacatcggtt gatgccgctc
     1981 acagttgatg tctgagaacc acagggctgg cagcagaaaa ccagccctgt ggtaaaatac
     2041 agtaatagcg gcgtacatca ggcacgccgc tcactcgtcc acagatcata tacgtcatta
     2101 tactgcgcga taacggaggg cttcagcacc cggtcatttc tgagcgggta tgaataccat
     2161 tgatgatgcg aagcagccac gggcagaaac gttcttcaca accaggtgcc tccatcttca
     2221 ctgcagtctg aatgtgccgg cttgttatcc accagaaatg gtatgctccg gtacatgcat
     2281 accccggaga ctataaacgc actgaatcat agcctcccac ctcagcagga ggcagtcagc
     2341 tttcaggcgg aaaaacgttg cagttaaaga tcgtgagaat cgctgtttcg ttgcatagca
     2401 actcgaacag ttgtagtgaa acattttacc tgcatcagca catggtcagg cgccgtctgt
     2461 atggcgtgga atactgatgt ttcggctgat ggcaagcctc attctctgtg gaaacagggg
     2521 cgcgcattag cttctcaacc agagagtgtc acaaagacaa atattgctga aaatcgcatt
     2581 catcgttcag gcgtgaatgc ccggagacaa aagccgctgt ccacacagtc cccctctgac
     2641 ctgaacaaaa agcatacaca caacaggctc acctgacccc gaaaaaccat aacttcagca
     2701 ataccagtga caccagcacg ttacctcagt gccacatctt cagaccgcga tcaatctgaa
     2761 atgtgactat cggggcaata tccctgcttt gtggtacacg ataaccagaa aacatcccct
     2821 gaaccgccag ccagaaagac tggccaacaa aaggggtaag ctgaaatcgt ttatggatta
     2881 catcgacata tgcctggcag agaatgtccg gaatgacgag gttatctttg cggagattaa
     2941 ggcaatggta tacgggccga cgttccatgc cgcgttacta cattcagccc ggacgtaaaa
     3001 tgcgcccgtc aaaaagaacg gttcgcttcg aaactcagcc aggataccac ctctccgcca
     3061 cacccgggaa gaagttgata tggtggttgc cggacaacag cgctcctgga ttgactgctg
     3121 catcactcaa ccacgctgaa tatcaaagga gagcgttacc ggttaaaaga gaaacgtaaa
     3181 gctgtggtga tgacccaaaa cgcaacgcct gtcagtgagg atgaaatggt gaaaagcgga
     3241 cagcatcagt aacggaagta tcttagcggg tacgaaaatg gcaggatcgt gacattgaca
     3301 gaatatctcc tgatcatcct gaatgactgc agttgctgta gaaaagtaag ctgtctccac
     3361 ccacggcacc acttaacagg cattccgtaa cttcccccag acttactatg acgcattatc
     3421 ggtgcactga aaacgggaga gtgcgcttaa aaccgcagac cgcatgcggg tgttactgca
     3481 tgacagtctg gccgtattgt gagatgtcgg caggaaccga tgtccgcgca ggggggaact
     3541 tcctgcagta cctgacgctt caaatggtat taaccgtagg gtgttgttgg ttccggatcg
     3601 cggaaatggg agcacaggtc tgctacgggg tgtacctctg acagtatatc cggctcgctc
     3661 atctgacagc ctctctcccg gaaaagacca aaaatgagat cgtctcctca acagcagaga
     3721 agaggtcttg acagacaggc tacattgcat acgatttatg aagaaaaaga cagcaaatat
     3781 ggaggttata gcggagaata ccctacgaaa ttcctatgaa aaacgattga aaaaaatatc
     3841 aaattcgatt cgtttttata tgctttttgg gagcgtggcg atgacgaact gttgcatgtg
     3901 cagaggagaa tgcacccgga agcaccagaa tcatccagaa aggcagaacg attacgcatc
     3961 aaaccaggag acagcaatat caaggtaata tactgattta aaatgaattt acagcaatac
     4021 gtaaaaacat tttttactta aaggagacag atgcgctgaa gagagtgcag gtatttttat
     4081 ataaaaatga gaagtttacg ggatgtgtat ccggcacact atgaccggag aatatcagtc
     4141 aagcttaaaa agacaataaa tacagtgagt taaatacaca gagaaaacgt agtatcagag
     4201 ggtacatctt acaacagaga ctgaatttcc ggtttttcat aaccggcgca ttccggaaag
     4261 tgcaggcggc ccggaggttt tctgacgcca ctacctggta gattgttctg tacatcatgt
     4321 acggaggttt ttgtttgctt ttttaaaatc tgcaaatgtg agaaaagcag gtgaggtgta
     4381 ttgtgggaaa ggaactggat gatatctgca ccagctgtcc gtatatcgat gctgtaaaac
     4441 ggcacaaaca acagctcggt gccatagagg aatatacaca gtggttaaaa aaagaaccac
     4501 gggcatctta tttttttctg ttccagctgt atacccgcat tcacaataca tttttctccc
     4561 aaaagcaaca actcccgttc actcccggtg gcactcactg cccggaacct gatgtcaccc
     4621 ttcgggactt aacgctttcc cctggctacc acagtgatta tgcccctcaa cccattccgg
     4681 agatggacag ttctgctgtt gttcccccaa ctaatgaaaa cacctcgcct ccggaagata
     4741 ctcccgacaa cactccggcc ggaggcaata ccggacaagc ggaaaagacc agaaacgcag
     4801 gactcacacc cattcctgaa aaacgttcag gaatgccccc ggaacatctg cggtttgcca
     4861 cggggtttcc atcgcaaccg aagattgccg gtcccagaga aaagccgatg cgcaccgtgc
     4921 atccggataa aatctaccgt gaaattatat ggttctgctc cagttacctg ctgaaatccg
     4981 gaccagaggc tacgaggacg attatcaact cagtattctc tgaatgggcc agcataaaca
     5041 atgattaccc ctcccccttt tcgtgggtgg acagcaggga cagtgaacag tgtgactggt
     5101 tatggaacgc catgcaggtc cggtgtgtgg gaaccccgct gaatcccctt accccggagc
     5161 agaaatactg gtttgcctgc gccacgtttg ataactggga gggctggaat gagcaacagg
     5221 tacagttttt actggaaagt aatcccagac ggaacagagc gaagtttacg caggtctcct
     5281 tccaggcccc caggattcag cataaagcga ttcttcttga tgagctgaaa agtgcccggg
     5341 agcaacaaaa aaggcgtgat gaacgtgctg atggttccgt ccctctgaaa ctgtccggaa
     5401 aaatccacaa acagcttgaa agtattgccc gaagtcgtgg tgtcctccca aaaaaactgc
     5461 tgaatgaaat gattgagcag gcgtaccatg atttggtggc aaccaggcag aacagccaaa
     5521 tcgattccag ataatttcag acagatatta tttccgtgaa tcccccgcca cctttccggt
     5581 gcgcggggtt ttgtcttttt tcaccgggaa tacatgtatg aatccgtctg atgccattga
     5641 ggcaattgaa aaaccgctct cctccctgcc ttactcactt tcccgtcaca tcctggaaca
     5701 tctgcgcaaa ctcaccagtc acgaacccgt gcctggcatt atgggtaaaa gcgggcccgt
     5761 aaatcctcac tctgtaatgc gctgtttcag ggggaggtca ccccggtcag tgatgttcac
     5821 gccggcaccc gggaagtgca gcgcttccgt ctgaatggcc atggtcacag catggttatc
     5881 actgacctgc ccggggtggg cgagagccgg gacagggatg cagagtatga agccctgtac
     5941 cgtgacattc tgctcgaact ggacctggta ctgtggctga ttaaagccga tgaccgtgcc
     6001 ctgtctgtgg atgagtattt ctggcgacac atcctgcacc ggggacatca gcgggtactg
     6061 tttgtggtga tgcaggccga taaaacggag ccctgccatg aatgggatat ggccggtatt
     6121 cagccttctc ccgcagaggc acagaacatt cgcgaaaaaa cggaggcggt attccgtctg
     6181 ttccggcccg tacatcgggt tgttgccgta tcggcccgca ccggctggga actggatacg
     6241 ctggtcagtg cgctcatgac agcacttccc gaccatgccg ccagtcccct gatgacccgc
     6301 ctgcaggacg agctgcgcac ggagtctgtc cggtctcagg cccgtgaaca gtttaccggt
     6361 gcggtggacc ggatatttga cacggcggag agcgtctgca ttgcctctgt tgcacgcacg
     6421 gttctgcgcg ccgtccgtga ctcggtggtc tctgttgccc gcgcggtatg gaactggatt
     6481 ttcttctgaa cccggtggtg atgtaccagg ctgttgttac agatttttaa ctccatgaaa
     6541 acactatgca aacgcagcca tacccgggtt gtccccttgc gttgtgtttc tcaagttaat
     6601 agaatgcatc gatcgttact atcatatcga tcgttataaa cgatcgtttg tagtaaatga
     6661 cctcctccgt ggttgtcatg aaaatgcgtc ttaggaaggt taaataatgc acgaaaagaa
     6721 catcgccctg ctttgtgatg aagccgaccg acttttgcaa ctgaacatta atctgctccg
     6781 gcaaatggtt gaggagccag atgtgttatc tgacagtaag aacgaaaaca gactgctttt
     6841 tgataaacag aaagcactga aaagaattga ggagctggag ggcgaacaaa tcaaaaccgc
     6901 ccgcagggag atggtgctgg ctgttgtcgg cacgatgaaa gcaggcaaat caaccaccat
     6961 caacgccatt gtggggcagg aaattctgcc taaccgtaac cgccccatga cctctgtacc
     7021 gacgctcatc cgccacgttc ccggaaaaac tgagccggtt ctccatctgg aacatattca
     7081 gcctgtccgc aatttattaa tcacactgca ggaaaaactc gccaccccgg caggacagca
     7141 ggtcgcacag accctgcagc aaaccgggga tacccgcgaa ctgctggata ttctgacgga
     7201 tgatggctgg ctcaaaaatg aataccacgg ggaggaggaa atctttaccg gactggcatc
     7261 gttaaacgat ctggttcgtc ttgctgcggc aatggggact gaatttcctt ttgatgaata
     7321 cgcagaagtg cagaaactgc cggtgatcga cgtggaattc agccatctgg tggggatgga
     7381 tgcatgccag ggaacactca cactgctgga tacccccggc cctaatgagg ccggacaacc
     7441 gcagatggaa gtgatgatgc gggatcaact gcagaaagcc tctgcggttc tggctgtgat
     7501 ggattacacc cagatgaact caaaagcgga tgaagacgtc cgtaaagagc ttaatgccat
     7561 tgctgacgta tcagccggcc gcctgtttgt actggtcaat aaatttgatg agaaagaccg
     7621 caatggcgat ggggcagatg ccgtacgcca gaaagttccg gcaatgctga acagcgatgt
     7681 gctgcccgcc tcccgcgttt atcccggatc ctcacgccag gcatacctgg ctaaccgtgc
     7741 gcttcatgag ttacggaaaa acggaaccct tcctgttgat gaagcctggg tcgatgattt
     7801 tatcagaggt gccttcggtt gcatgaaaaa agaatacgtc tgtaaagaca gtgaactggc
     7861 aactgaaggg gcgacagacc tgtgggaagg ctcacttatc gatcaactga taacggaagt
     7921 catacagagc tcacattcca gagctgcggc actggcggtt gactctgccg ccgcaaaact
     7981 gatgcagaat gcagaaaata tcagtgaata cctgttgtta cgccatcagg ggctacagca
     8041 aagcattcag tcactgcagt cgcatatcac cagcctgctt gcggatatcc gggaaatcgc
     8101 ggactgtcag gagcagatga ccactgatgt cagaatggcc atggaggaga tcgatactaa
     8161 aacccgggaa ttactgacgg gggtctgcac ctcactggaa gaggagctga atgactattt
     8221 cagaagcggt aaacgcaaag aacagcaaat gctggaggaa gaagacgcag aacaacgaag
     8281 gtctcagtcc ggattatggg ggaaaatttc tcaatggtcc ggtatcaaca accagggccg
     8341 ggaggattac aggaaacgag attttgcccc ggacagcccc gaaataaaat tcagtgatcg
     8401 caggg
//
LOCUS       DAZFXT010000179         8367 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.179, whole genome shotgun sequence.
ACCESSION   DAZFXT010000179 DAZFXT010000000
VERSION     DAZFXT010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8367
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.179"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..176)
                     /locus_tag="ACHKW0_004494"
     CDS             complement(<1..176)
                     /locus_tag="ACHKW0_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HFO7477188.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMD"
     gene            489..578
                     /gene="kdpF"
                     /locus_tag="ACHKW0_004495"
     CDS             489..578
                     /gene="kdpF"
                     /locus_tag="ACHKW0_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HFO7477189.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            578..2251
                     /gene="kdpA"
                     /locus_tag="ACHKW0_004496"
     CDS             578..2251
                     /gene="kdpA"
                     /locus_tag="ACHKW0_004496"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HFO7477190.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2274..4322
                     /gene="kdpB"
                     /locus_tag="ACHKW0_004497"
     CDS             2274..4322
                     /gene="kdpB"
                     /locus_tag="ACHKW0_004497"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HFO7477191.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4331..4903
                     /gene="kdpC"
                     /locus_tag="ACHKW0_004498"
     CDS             4331..4903
                     /gene="kdpC"
                     /locus_tag="ACHKW0_004498"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HFO7477192.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4896..7580
                     /gene="kdpD"
                     /locus_tag="ACHKW0_004499"
     CDS             4896..7580
                     /gene="kdpD"
                     /locus_tag="ACHKW0_004499"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HFO7477193.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSYDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7577..8254
                     /gene="kdpE"
                     /locus_tag="ACHKW0_004500"
     CDS             7577..8254
                     /gene="kdpE"
                     /locus_tag="ACHKW0_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HFO7477194.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1749 a         2161 c         2439 g         2018 t
ORIGIN      
        1 tgatccatcc acaccggttt atcgctggtt ttcgaccaga cgcgatgcag gtagctgtaa
       61 aagcgggcgc ggtctttcca gaaaagcatg aaaggcagcg ccagaacgcc cgctgcaacc
      121 gcataagtac ggcgtaaaaa gataagccat gcaggatatt ctctgtagag ttccatactt
      181 ttctccccct tttgtatcta cccggtgaat ggcaccggaa aaatgaattt gtttatctga
      241 tgaaaatagt accgcctttt gtgtaatttt actactcatc cgaccactta tttttgctta
      301 ttgatggttt atttacattc atcctgtaat taagttacac aaaagttaaa ttaatactaa
      361 acattagtta aatcatggct tttgccattt ttatactttt tttacacccc gcccgcagat
      421 ttttgcgaaa tctttgcagc cagaattcta cccttccggt atcactttta ggccactgga
      481 ggtgcactgt gagtgcaggc gtgataaccg gcgtattgct ggtgttttta ttactgggtt
      541 atctggttta tgccctgatc aatgcggagg cgttctgatg gctgcgcaag ggttcttact
      601 gatcgccacg tttttactgg tgttaatggt gctggcgcgt cctttaggca gcgggctggc
      661 gcggctgatt aatgacattc ctcttccagg tacagcgggc gttgaacgta tactctttcg
      721 tttacctggc gtctctgacc atgagatgaa ctggaagcaa tatctttgtg ccattctcgg
      781 cctgaacatg ctggggctgg cggtgctgtt ttttatgttg ctcggtcagc actatctgcc
      841 gcttaatcca cagcagttgc cggggctgtc gtgggatctg gcgctgaata ccgccgtcag
      901 ctttgtcacg aataccaact ggcaatctta cagcggtgaa accacgttga gctatttcag
      961 ccagatggct ggcttaacgg tgcaaaactt tctttctgcc gccagcggga ttgcggtgat
     1021 ttttgccctc atccgtgcgt ttacccgcca gagcatgagc acactcggga atgcctgggt
     1081 cgatctgcta cgcatcacgt tatgggtgct cgtccctgtg gcgttgttga tcgcactgtt
     1141 ttttattcaa caaggtgcgc tgcaaaactt tcagccttat caggctgtga ataccgttga
     1201 aggagcgcaa cagctgttac ccatggggcc tgtagcttct caggaagcga tcaagatgct
     1261 cggtactaac ggcggtggct tctttaatgc caactcgtcg catccgtttg aaaacccaac
     1321 cgcactgacc aacttcgtgc agatgctggc gatcttcttg atcccaacgg cgctgtgctt
     1381 tgcctttggt gaagtgacgg gcgatcgccg ccaggggcgc atgttgctgt gggcgatgtc
     1441 agtgattttt gtcatctgcg taggcgtggt gatgtgggca gaagttcagg gtaatcctca
     1501 tctgctggcg ctcggcgcag acagcagcat caatatggaa ggtaaagaga gccgtttcgg
     1561 cgtgctggtc agtagcctgt ttgcggtcgt gactacggcg gcttcctgtg gcgcggtgat
     1621 tgcgatgcat gattcgttta ccgctctcgg tggcatggtg ccgatgtggc tgatgcaaat
     1681 tggtgaagtg gtgttcggcg gtgtcggttc tggtctttac ggcatgatgc tgtttgtcct
     1741 gctggcggtg tttattgccg ggctgatgat tggtcgtaca ccggaatatc tgggtaaaaa
     1801 aatcgacgta cgcgagatga aactgactgc actggcgatt ctggttaccc cgacgctggt
     1861 gctgatgggc gcggcgctgg caatgatgac cgacgccgga cgtagcgcca tgctcaaccc
     1921 tggcccgcat ggttttagcg aagtgctgta cgccgtgtca tccgccgcta acaacaacgg
     1981 cagcgccttt gccggattaa gcgccaactc cccgttctgg aactgtttac tggcgttctg
     2041 catgtttgtc ggtcgcttcg gggtgattat cccggtgatg gcaattgccg gttcgctggt
     2101 gagtaaaaag agccaacccg ccagctccgg cacgctgcca acgcacggcc cgctgtttgt
     2161 tggcctgtta atcggcaccg tgttgctggt tggcgcactg acctttatcc ctgccctggc
     2221 gcttggtccg gtggcggaat atctctcctg atgatattga gtgagcactg aatatgagtc
     2281 gtaaacaact ggcgctattc gaaccaacac ttgtcgttca ggcgctgaaa gaagcggtga
     2341 aaaaattaaa cccgcaggcg caatggcgca atccggtgat gtttatcgtc tggatcggca
     2401 gtctgctgac cacctgtatt agcatcgcga tggcaagcgg tgcgatgcct ggcaatgcgc
     2461 tgtttagcgc ggcaattagc ggctggctgt gggtcaccgt actgttcgct aatttcgccg
     2521 aggcgctggc agaaggccgc agtaaagcgc aggccaacag tcttaaaggg gtgaaaaaaa
     2581 ctgcctttgc ccgcaagctg cgtgagccga aatatggcgc tgcggcggac aaagttcctg
     2641 ccgatcaact gcgtaaaggt gacatcgtgc tggtagaagc tggcgatatt atcccctgcg
     2701 atggtgaagt cattgaaggc ggcgcatcgg tcgatgaaag cgccatcacc ggggaatcgg
     2761 caccggtgat ccgtgaatcc ggcggcgatt ttgcctccgt caccggcggc acgcgtattc
     2821 tttctgactg gctggtgatt gagtgtagcg ttaaccccgg tgagacattt ctggatcgga
     2881 tgatcgcgat ggtggaaggc gcacagcgac gcaaaacacc gaacgagatt gccctgacta
     2941 tcctgctgat tgctctgacc atcgtctttt tactggcaac cgccacgctg tggccgtttt
     3001 ccgcgtgggg cggtaatgcc gtgagtgtaa cggtactggt ggcgctgctg gtctgtctga
     3061 tcccaaccac tattggcggc ctgttgtcag cgatcggcgt cgccgggatg agtcggatgc
     3121 tgggcgcgaa tgtgattgcc accagcggac gtgcagttga agcggcaggt gacgttgacg
     3181 ttctgctact ggataaaacc ggcaccatca cactcggtaa ccgtcaggcg tcggagttta
     3241 tccctgcgca gggcgtggat gaaaaaacgc tggctgacgc cgcacaactg gcttcgctgg
     3301 ctgatgaaac gccggaaggc cgcagtattg tgatcctcgc caagcaacgt tttaacctgc
     3361 gcgagcgcga tgtgcagtcg ctccatgcca cctttgtacc gtttactgcg caaagccgga
     3421 tgagcgggat caacatcgac aaccgcatga tccgtaaagg ttctgtcgat gccattcgcc
     3481 gccatgttga ggctaacggc ggtcacttcc ctaccgatgt tgatcaaaaa gtcgatcagg
     3541 ttgcgcgtca gggggccacg ccgctggtgg tggtggaagg ttcccgtgtg ctgggcgtta
     3601 ttgcgctgaa agatatcgtc aaaggcggta ttaaagagcg cttcgcccag ctgcgcaaaa
     3661 tgggcattaa aacggtgatg attaccggcg ataaccgtct gactgccgcc gccattgctg
     3721 cggaagcggg cgtcgatgat tttctcgccg aagcgacacc ggaggccaag ctggcattga
     3781 ttcgtcagta tcaggcggaa ggccgtttgg tggcgatgac cggcgacggc accaacgatg
     3841 ctccagcgct ggcgcaggca gatgtcgcgg tggcgatgaa ctccggtact caggcggcga
     3901 aagaagcggg caatatggtc gatctcgact ctaacccgac caagttgatc gaggtggtac
     3961 atattggcaa acagatgctg atgacccgtg gctcgctgac caccttcagc attgccaacg
     4021 atgtggcgaa atacttcgcc attattccgg cggcgttcgc ggcaacgtat ccgcagttaa
     4081 atgcgctgaa catcatgcgc cttcattcgc ccgactccgc cattctcagt gcggtcattt
     4141 tcaacgcctt gattatcgtc tttttgattc ctctggcgtt aaaaggcgtg agttataaac
     4201 cgcttaccgc ttctgccatg ttgcgccgta acttatggat ttacggtctg ggtgggctgc
     4261 tggtgccgtt tattggtatc aaagtcattg atttactgct gaccgtttgc ggtctggtgt
     4321 gaggtttacc atgcgtggct tacgtccggc attatcgaca tttatctttc tgttattgat
     4381 tactggcggc gtttacccgc tgctgaccac cgtgctgggg caatggtggt ttccctggca
     4441 ggccaacggt tcgttgattc gtgaaggtga tacggtgcgg ggttcggcat taatcgggca
     4501 gaattttacc ggcaacggct attttcaggg tcgcccgtcg gcaacggcag aaatgcccta
     4561 taatccacag gcttctggcg ggagcaatct ggcggtcagt aaccctgagc tggataaaca
     4621 aatagccgca cgcgttgctg cattacgggc cgctaacccg gatgccagta cgaacgttcc
     4681 ggttgaactg gtgacggcat cggcaagcgg gctggacaat aatatcaccc cgcaagcggc
     4741 ggtctggcaa atcccacgcg tggcgaaagc gcgtaatctc agcgttgaac agctcacgca
     4801 actgatcgca aaatacagcc aacaaccgct ggtgaaatat atcggccagc cggtcgtcaa
     4861 cattgttgaa ctcaatctgg cgctggataa acttgatgaa taacgaaccc ttacgtcccg
     4921 accccgatcg tctgctggaa caaactgccg cgccgcatcg ggggaagctg aaagttttct
     4981 tcggtgcctg tgcaggcgtc gggaagacct gggcgatgct ggcagaagcc cagcgactgc
     5041 gggcgcaagg gctggatatt gtggttggcg tggtagaaac ccatgggcgg aaagataccg
     5101 ccgccatgct tgaagggctg gctgttctgc cgcccaaacg ccaggcgtac cgtgggcggc
     5161 atatcagcga gtttgatctc gatgccgccc tcgcccgccg tccggcgctg atcttaatgg
     5221 acgaactggc gcacagtaat gcgccaggtt ctcgtcatcc taaacgctgg caggatatcg
     5281 aagaactgct ggaagctggc attgatgttt tcacaaccgt caacgttcag catctggaaa
     5341 gtctgaatga tgtggtcagc ggcgtcaccg gaattcaggt acgggaaacc gtgcccgatc
     5401 cttttttcga tgccgccgac gacgtagtgc tggtggactt gcccccggac gatctgcgcc
     5461 agcggctgaa agaaggcaaa gtctatattg ccgggcaggc ggagcgcgcc attgaacatt
     5521 ttttccgcaa aggcaatctg atcgccctgc gtgaactggc actgcgccgt actgccgatc
     5581 gcgtagatga gcaaatgcgc gcctggcggg ggcatcctgg tgaagagaaa gtgtggcaca
     5641 cgcgcgacgc gatcctttta tgcatcgggc ataacaccgg cagtgagaaa ctggtccgcg
     5701 cggcggcgcg gttggcctcg cgcctgggta gcgtctggca cgcggtgtat gttgaaaccc
     5761 ctgccctgca tcgcttaccg gaaaaaaaac gtcgggcaat tctcagcgcc ttacgtctgg
     5821 cgcaggagct gggcgcggag acggctacac tttctgatcc agcggaagaa aaagcagtag
     5881 tgcgttatgc ccgtgaacat aatctcggca agattattct cggtcgcccg gcctcgcgcc
     5941 gctggtggcg tcgggaaacg tttgctgacc gactggcgcg catcgccccc gatctcgatc
     6001 aggtgctggt cgcgcttgat gaaccacccg cccgcacgat taacaacgcg ccggatagcc
     6061 gctcttttaa agacaagtgg cgggtacaga ttcagggatg cgtggttgcc gccgcgttat
     6121 gcgccgttat cactttaatt gccatgcagt ggctgatggc gtttgatgcc gccaacctgg
     6181 tgatgctgta tctgcttggc gtggtggtgg tggcgctatt ttatggacgc tggccttcgg
     6241 tggttgctac cgtcattaat gtggtgagtt acgatctctt ttttatcgcc ccacgcggca
     6301 cgctcgctgt ctctgatgtg caatatctgc tgaccttcgc ggtgatgtta accgtcgggc
     6361 tggtgatcgg gaaccttact gcgggtgtgc gttatcaggc gcgggtagcc cgttaccgcg
     6421 agcaacgcac acggcactta tatgaaatgt cgaaagctct ggcggtgggc cgcagtccgc
     6481 aggacatcgc cgccaccagc gaacaattta ttgcctccac gtttcatgcc cgcagtcagg
     6541 tgttgttgcc cgatgacaac ggtaaattgc agccgttaac acatccgcaa ggaatgacgc
     6601 catgggacga tgccatcgcg cagtggagtt ttgataaagg cctgcctgcg ggtgcgggca
     6661 ccgacacgtt acccggcgtg ccgtaccaga ttttgccgct aaaaagcggc gagaaaacct
     6721 acggactggt ggtggtggaa ccggggaatc tgcgtcagtt gatgatcccg gagcagcagc
     6781 gcctgctaga gacgtttacg ctgttagtcg ccaatgcgtt tgagcggctg acgctgaccg
     6841 ccagtgaaga acaggcgcgg atggcaagcg aacgtgaaca gatccgcaac gccctgctgg
     6901 cggcgctttc gcatgattta cgcacgccgc ttacggtgct gtttggtcag gcagaaatct
     6961 taacgctcga tctggcaagc gaaggatcgc cccacgcccg ccaggccagc gagattcgtc
     7021 agcatgtgct gaacactacc cgactggtga ataatctact ggatatggcg cgaattcagt
     7081 ccggcggctt taatttgaag aaagagtggt taacgctgga agaagtagtc ggcagcgcgc
     7141 tgcaaatgct ggaaccgggt ttatcgtcgc ccatcaatct ttctctgcca gaaccgctga
     7201 ccttaatcca cgttgacggg ccactctttg aacgggtgct gattaatctg ctggagaacg
     7261 cggtgaaata tgcgggtgcg caggccgaaa ttggtatcga tgcccacgtt gagggcgaaa
     7321 atctacaact ggatgtctgg gataacggcc ccggtcttcc gccaggccag gagcagacga
     7381 tatttgataa gtttgctcgc gggaataaag agtcggcagt accgggggta gggcttggac
     7441 tggcaatttg tcgggcgata gtggatgtac acgggggcac tattaccgcg ttcaaccgac
     7501 cggaaggtgg tgcctgtttt cgtgttacac ttccccagca aaccgctcct gaacttgaag
     7561 actttcatga ggatatgtga caaacgttct gattgttgaa gatgaacagg ctattcgtcg
     7621 ctttctgcgc acggcgctgg agggcgacgg gatgcgcgtc tatgaggccg aaacgctgca
     7681 acgcggcttg ctggaagcgg ctacccgtaa gccagatttg attattctcg atctcggcct
     7741 gcccgatggt gatgggattg agtttatccg cgacctgcgc cagtggagcg cggtgccggt
     7801 gattgtgctt tccgcacgca gcgaagagag cgacaaaatc gccgcgctgg atgccggagc
     7861 ggatgattat ctgagtaagc cgtttggcat tggcgaattg caggcccgtc tgcgcgtcgc
     7921 tttacgccgc cactccgcca ccgccgcgcc cgatccgctg gtgaaatttt ccgatgttac
     7981 cgtcgattta gccgcccgcg tgattcaccg gggtgaggaa gaggtgcatc tcacaccgat
     8041 tgagttccgc ctgctggcgg tgctgctgaa taatgccggg aaagtgctca cccagcgcca
     8101 gttactcaat caggtgtggg ggccaaacgc ggtcgaacac agtcactatt tgcgtattta
     8161 tatgggacat ctgcgacaaa aactggaaca ggatcccgct cgcccccgcc atttcattac
     8221 tgaaaccggt attgggtatc ggtttatgcc atgaatgttt attcaataaa tgcgcttaaa
     8281 taattattaa tttattatta aacctgccaa aaatattatt atttggcagg tttaatttct
     8341 taacgcaaat ataaaacaca aaattac
//
LOCUS       DAZFXT010000180         8327 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.180, whole genome shotgun sequence.
ACCESSION   DAZFXT010000180 DAZFXT010000000
VERSION     DAZFXT010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8327
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.180"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            268..1887
                     /locus_tag="ACHKW0_004501"
                     /pseudo
     CDS             268..1887
                     /locus_tag="ACHKW0_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            1967..2259
                     /locus_tag="ACHKW0_004502"
                     /pseudo
     CDS             1967..2259
                     /locus_tag="ACHKW0_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312320.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
     gene            complement(2299..3333)
                     /locus_tag="ACHKW0_004503"
     CDS             complement(2299..3333)
                     /locus_tag="ACHKW0_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase"
                     /protein_id="HFO7477195.1"
                     /translation="MKDYLQTVTGPVAREDMGLTLPHEHLFNDLSSVVDAPCYPFSQQ
                     LVDKKVTAEIQWALKHDPYCCADNMDRKPIEDVIFEINNFISLGGRTIVDATGSESIG
                     RDAQALREVALKTGLNIVASSGPYLEKFESQRIHKTVDELATTIDKELNQGIGDTDIR
                     AGMIGEIGVSPTFTEAEHNSLRAASLAQINNPHVAMNIHMPGWLRRGDEVLDIVLGEM
                     GVSPNKVSLAHSDPSGKDVAYQRKMLDKGVWLEFDMIGLDITFPKEGIAPGVQETADA
                     VAHLIELGYADQLVLSHDVFLKQMWAKNGGNGWGFVPDVFLAYLAERGVDKTILKKLC
                     IDNPGRLLTA"
     gene            complement(3336..4301)
                     /locus_tag="ACHKW0_004504"
     CDS             complement(3336..4301)
                     /locus_tag="ACHKW0_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HFO7477196.1"
                     /translation="MKKNKYSTPLWVLATILAGMLSPMQSAVNGQLGHWLQDGNACAV
                     ISFASGLVVMFFIIMARKEIRQQFAAIPSLIKNRKVPLWNWFAGLCGAMVVFSEGASA
                     SALGVATFQTALISALLLSGLLCDRFGIGVDEKKYFTPYRIIGALFAVIATIFVVSPQ
                     WHSTSFILLAILPFLAGLLAGWQPAGNAKVAEATGSMLVSITWNFIVGFCVLGTALAI
                     RVALGHVTVQLPDVWWMYLGGPLGLMSIGLMAILVRGLGLLMLGVASTAGQLLGSVLI
                     DVLIPSLGNTVYLVTIIGTLFALVGAIVTTIPEYRASKTMKKMEV"
     gene            complement(4358..5116)
                     /locus_tag="ACHKW0_004505"
     CDS             complement(4358..5116)
                     /locus_tag="ACHKW0_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HFO7477197.1"
                     /translation="MNNTKKSLKVLFIGESWHIHMIHSKGYDSFTSSKYEEGATWLLQ
                     CLKNSQVDVTYMPAHTVQIAFPEDVAQLEQYDAIVISDIGSNTFLLQNDTFYQLRIKP
                     NALELIKEYVNNGGGLLMIGGYLSFMGIEAKANYKNTVLADVLPVTMLDGDDRVEKPE
                     GVIAQPSQPDHPVIKGFSEYPFFLGYNRAIAKENAEVVLTINNDPLLVFGNYHNGKIA
                     CFMSDCSPHWGTQQFMSWPFYTALWVNILTHIAR"
     gene            complement(5130..6344)
                     /locus_tag="ACHKW0_004506"
                     /pseudo
     CDS             complement(5130..6344)
                     /locus_tag="ACHKW0_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462448.3"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
     gene            complement(6540..6900)
                     /locus_tag="ACHKW0_004507"
                     /pseudo
     CDS             complement(6540..6900)
                     /locus_tag="ACHKW0_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6962..7652
                     /locus_tag="ACHKW0_004508"
                     /pseudo
     CDS             6962..7652
                     /locus_tag="ACHKW0_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(7840..8327)
                     /locus_tag="ACHKW0_004509"
                     /pseudo
     CDS             complement(7840..8327)
                     /locus_tag="ACHKW0_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310819.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
BASE COUNT         2284 a         1898 c         1953 g         2192 t
ORIGIN      
        1 aatcagttgc agagctgtaa tgatatctcc gggagtgctg cgttggtagg ggcatcccgg
       61 gatagagaaa ttttagcact ttgccctgaa tgattgtaga cgtcccgagt actcgtaccg
      121 gtctgagtta ttacagttag atattcactt tttctcccgc atacttcaca tctgtcaacg
      181 acggatgaaa agtggttctt ccgcaacctg ggtggaagaa aatggatgaa actttctggc
      241 gaacaaggat ctaagctgtt ttaagttatg ggcaacgcaa tgcactctct taagacactt
      301 ctacagttac cttgcggatg gcgatgcagt cgacaaatta ttagctctga cggtataacc
      361 ctccatctcc acggaaaacg caaaacagca caatgtcctg aatgctttaa gcgtagcgac
      421 tctgttcata gttgtcgtcg gcgccggata cagcatctac cctgctcagg gcagacgcta
      481 tggcttgtat tttccgtccg ccactggtac tgccgtaacc cggcttgttc acgtaaaatt
      541 tttgccgagt cgcttgctcc cttcgccagt tcacaccagc agtcttcaca ggcgttacaa
      601 aattaacaac gtcaactggg attaatagct ggaggtgagg ctggaaaacg ggctgcaacg
      661 gcagtgggtc tccgttgcag tgctgatact cttcttcgca ggcttatcaa taccccggag
      721 acgaaacagt caggcgcgcc tcatgtcggt attgatgagt gggcgtggca tcggggccac
      781 tgctgcggta tgttaatagt caatcctgat actcaccgtc ccctcgtcct gcttcccggc
      841 cgtgatcagc gtacgctggc gacctggttc agaaaatatc cggaaataca ggttgtctcg
      901 cgtgatcgca gtggagtcta tgcgacagca gcacgtgaag gtgcacctca ggccagacag
      961 gtggccgatc gatggcacct gctaaaaaat attggtgatg agcctgaacg aatgatgtac
     1021 agatatatgc ctctgatacg tcttgttgtc agagagttat cactgaagaa atcacctgag
     1081 ccagaaatat ctgtgcctgt agcatcgctc cgtcgtctgg aacgccttaa acagcacatc
     1141 cgcaaaaaac ggcatcagcg ttggacagag gttatggccc tgcataacaa gggatgtagt
     1201 ttcagggaaa tatcccgtat tacaggcctg tcgcgagtga cagtcagtcg ctgggtgggt
     1261 tcaggaacat tccctgaaat gtcaactagg cctccaaagc gagggcttct ggacccatgg
     1321 agggagtggt taaaagagca acgagaatgt ggtaattata actccggccg gatatggcgg
     1381 gaaatggtgg ccaggggggt tacaggcagt gaaaccatcg tcagggatgc tgttgccaaa
     1441 tggcataaag gctggatccc accggttact actgccgcaa gacttccttc agtgtcccgg
     1501 gtaagccgct ggttgatgcc ctggagaata atcaggggtg aagaaaatta tgcgttccga
     1561 tttattagtc tgatgtgtga aaaagaaccg gagttgaaaa tagcgcagca actggtactc
     1621 gagttctacc gtattctgaa aacctaaaat aaatcacagc taagcagctg gttcactcgg
     1681 gtccacgaaa gcggctcagc agaacttcgg tgcgtagctg cggggatgga agctgatgct
     1741 gcggctatat gtgaggcaat cagcagtcgc tggagtactg gtgttgtcga aggtcatgta
     1801 aatcgcctga aggtgctgat acgccagatg tatggtcgag ccggacttga actacttagg
     1861 cggagggtaa tgagtccact ggcatgaaaa tcttcaccta ggttgaggaa gagccacttt
     1921 tgcactgttc gtgacagtat ggcctttatg tactcacttt gttaccggta gctggatcat
     1981 cactaccgtg ccgcaaatgg tggtgaacag catcaattgt ggaatgaatg ttgccgaagc
     2041 gaccaatgcg cctcgtttcc accatcagtg gttgccggac gagctgcgtg ttgaaaaggg
     2101 tttagtccgg atacgctcaa gctgctggaa gcaaaaggtc agaaagtggc gctgaatgag
     2161 gcgatgggca gcacgcaaag cattatggtt gggtcggatg gtgagttgta cggcgcatcc
     2221 gactcgcgct tggtggatga tttaacggca gggtattaat tgacatcccc cggcgcaatg
     2281 tgccggggga taatggtttt atgcggtcaa taatcgtccg ggattatcga tacagagttt
     2341 tttgaggatc gttttatcga cgccgcgctc cgccagatag gccagaaaaa catctggaac
     2401 aaatccccag ccattaccgc catttttagc ccacatttgt ttaaggaaga catcgtggct
     2461 gagaacaagc tgatcggcgt atcccaactc aatgagatga gcgacggcat cggcagtctc
     2521 ctgcacccct ggcgctattc cctctttcgg gaaggtaatg tctaggccaa tcatgtcgaa
     2581 ttccagccag acacctttat caagcatttt ccgctgatac gccacgtctt ttcctgacgg
     2641 atccgagtgt gcgagagaga ctttatttgg cgagacgccc atttcaccta acacaatgtc
     2701 gagtacttca tcaccgcgac gaagccagcc cggcatgtga atattcatcg ccacatgagg
     2761 attgttaatc tgtgccagcg aggcagcccg caagctgtta tgctcggctt cggtaaatgt
     2821 cggtgagaca cctatttcac cgatcattcc ggcacgaata tccgtatcgc caatcccttg
     2881 attcaattct ttatcgatcg ttgtcgctag ttcatcaacc gttttatgaa ttctctggct
     2941 ttcaaatttt tccaggtagg gaccggagga agcaacaata ttcagacctg tttttaatgc
     3001 gacttctcgt aatgcctgcg catctcgtcc gatagactca gaccctgtgg catcaacaat
     3061 ggtgcgacca ccgagcgaga taaagttatt aatttcgaag atcacatctt caataggttt
     3121 gcggtccata ttatcagcgc agcaataagg atcgtgtttt aatgcccact ggatttctgc
     3181 cgtgactttt ttatcaacaa gctgttgtga aaaaggatag caaggagcat ccacaacaga
     3241 ggaaagatcg ttgaacagat gttcatgagg aagtgttagc cccatatctt cacgggcgac
     3301 ggggcccgtc acagtttgta gataatcttt catgattata cctccatttt cttcattgtt
     3361 ttcgaggctc tgtattccgg aatagtggta acaattgcac cgaccagtgc aaacagtgtt
     3421 ccgatgatcg tgaccagata aacggtattg cccagcgatg gaattaatac gtcaatcaat
     3481 actgatccca gtaactggcc cgccgttgaa gcaaccccca gcatcagtaa ccccagaccc
     3541 ctgaccaaaa tggccattaa accgatagac attagtccaa gcgggccgcc aagatacatc
     3601 caccagacat ctggtaattg aacagtgacg tgccctaatg cgacacgaat agctagcgct
     3661 gtgcctaaaa cacaaaatcc aacgatgaaa ttccaggtga ttgaaaccag catggatccc
     3721 gtagcttcag ctacttttgc atttccagcg ggctgccatc ctgcaagcaa accagctaag
     3781 aacggaagaa tggcaagtaa aatgaaagag gtagaatgcc actgcggtga cacaacaaaa
     3841 atcgtagcaa taaccgcaaa taatgcaccg atgatgcgat aaggggtaaa gtattttttc
     3901 tcgtcgacac cgataccaaa gcgatcgcaa agtaaaccag acagcaatag cgcggagatc
     3961 agtgcggtct gaaacgttgc gaccccgagt gcgctggctg atgcgccttc agaaaatacc
     4021 accatcgctc cacagagacc ggcaaaccag ttccagagag gaacttttcg gtttttaatc
     4081 agagaaggaa ttgccgcaaa ttgttgccgg atttcttttc gcgccataat aataaaaaac
     4141 atcacgacta aaccactggc aaaggagata actgcgcatg cattaccgtc ctgtaaccag
     4201 tgacctaatt gaccattcac cgctgattgc ataggggaga gcatacccgc aagaatcgtg
     4261 gcaagcaccc ataagggagt tgagtactta ttctttttca tacatcaatc cttctattat
     4321 agtaaatgga gtaactggtg ttaccccatt attattatta tcttgcaata tgagtcagta
     4381 tatttaccca caacgcggta tagaacggcc aggacataaa ctgctgtgta ccccagtgcg
     4441 gtgaacagtc actcataaag caggctattt tcccgttgtg atagttacca aagaccagta
     4501 atggatcgtt gtttattgtt aataccacct cagcattttc tttagcgatc gcccggttat
     4561 aacctaggaa aaaaggatat tcagagaacc ctttaataac cggatggtca ggttgtgatg
     4621 gctgtgcaat aacaccctct ggtttttcga cacgatcgtc accatctaac atcgtaaccg
     4681 ggagcacatc agccagaact gtatttttat aattggcttt tgcttcaatt cccatgaatg
     4741 agagatagcc gccaatcatc aataacccac caccgttatt cacatattct ttaatcagtt
     4801 cgagagcgtt tggttttatg cgaagctgat aaaaagtatc attttgtagc aggaaagtat
     4861 tactaccaat atcactaata acgatggcat catattgttc taactgtgcc acatcttcag
     4921 ggaaggcaat ttgtaccgta tgtgccggca tataggtgac atctacctga ctatttttca
     4981 gacattggag taaccaggtc gcaccttctt catatttact tgaggtaaaa ctgtcatatc
     5041 cttttgaatg aatcatatga atatgccagg attctccaat aaagagcacc tttagtgatt
     5101 ttttggtgtt attcattctc tgtcccttct taatgcgtaa aaatagtttg ctgataatgt
     5161 atactctgaa ttcgatgtat cacagattca tgttcaggca tgtcggaagc attgcttgtc
     5221 tccaccgcta gggatgcaaa ggcgctggca taacacagtg ctgattccag tgattttcct
     5281 ttcgcaagtc ctgatgccag agcgccgtta aaagcatccc cggctcctgc cgtattttta
     5341 acgaccgctg gaaacgctgg cgaatgaatg aattttttgc catcaaatgc gagcgagccc
     5401 ttgctaccga gcgtgataac ggtgtttttt acccctttat tgtggatagc ttctgcagct
     5461 cgtttagctg attcaagatc gtgtacgtct atgccggaca gtaaaccagc ttctgtttca
     5521 ttgggggtga tataatcaac atcctgaata agttcgttga cgatatcatt atagggtgcc
     5581 gggttaataa tgaccgggat actgtttttt tgcgccaatg tgatcgcttg ttgcagagcg
     5641 gtataatttg tttcgagctg cagaagtatg acatcagaat taataatcgc ttctttctga
     5701 atagttattt catcggacga cattgtcatg ttggcaccgg gatagatcgc aataacatta
     5761 tcgccagtac cttcattaac aaggatcgtg gcagttccgg tttgcgtttc tttcgtttga
     5821 taaattatac ttttataaat cttagacgaa ttcataaagt tgatcgcata atcactaaag
     5881 tgatctgtac ctacttttgt tatgaaataa acatcagtat cggcaaagct tgctgccagc
     5941 gcctggttac aaccttttcc ccccggtgaa aatataaaat tacttgccag aagcgattct
     6001 ccgatagtgg gaagcctcgg caaataactg atgatatcaa cattaaacga accaataacg
     6061 catacttgac ttttcatagt tgagtggcct ttttttattc cctaggctgt atcataattt
     6121 tcaagcgcat aagcgatttc atttttcgca atagtatcaa gcgagtctag gacgataaaa
     6181 gcaccgccat gatatcgagt cagatagcca agtctggcaa gttcattaag atctgatcga
     6241 atagtttctt tcgttacatt gaggctattg gacagatcgg atacttttac agaaccgaat
     6301 ttactgagtc gtttcaatat ttcatgatgg cgttcaaatt tcatgcttca cctcacttca
     6361 gttacgttaa tatctgtcga tgtatattga tgtgataatc accataaagt ggcggggaaa
     6421 agcaaaaaaa atcaaaaata gccggagaaa aggtaagcgt taatggagaa tcatattgat
     6481 gcttatttat tggtgagaac tacgttccat ggcaggagtt catcaatacg gttggaaggt
     6541 tattccggca gtacgctcag gatatggcgc agatactctt ccggatcaat gtcgttcaga
     6601 aggcaagtgc cgataagccc atacaacagt gcactgcgct cgccgtcgaa gaacataaag
     6661 tttttatttc cgagacagat ggcacaaagg gctttttccg cggcattatg tctgcctccg
     6721 cctgaccgtc aacactgtaa taacagagtg cgccccactg attcagtaca cagctgaacc
     6781 attctcccag tctggatttt tggacagcgt ggcatttttc tccaccatcc acgcatgcag
     6841 tgagtccagt aacagtttgc tcttcctctg cctgaccaca agatgttctg gttctggcat
     6901 actgaatttg gccacctgag cataggtaat atgctcacct tagaacaaca cagatgctcc
     6961 aatgaaaaaa ggaaatttca gcgcagagtt taaacgcgaa tccactcaac tagttgttaa
     7021 ccagaacttt acggtggcag gtaccatcca aagctatgga tgtcggtctt tccacaatga
     7081 caagataagt caaacaactg cgtaatgagc atcagagcaa aacaccaaaa gcctctctga
     7141 tgacaccaga acaaatcgaa atacgtgagc tgaggaaaaa gcggcaacgt attgaaatag
     7201 agaatgaaat attagaaaag gctacggcgc tcttgatgtc agactcccta aacagttctc
     7261 gataatcgag aagctcagag cgcattatct ggtgatagca ctctgccatg tgtgcaggat
     7321 tcatcgaagc agctacagag attggaaaaa ccgtcctgaa aaaaagacgg tagacgggct
     7381 gtattacgta gtcaggtaat tgagcttaat ggcggagcaa ggaacatcgc cacaattgca
     7441 acccggagag actaccagac gggacgcaag cttgctggca gtctcatgaa agaacagggg
     7501 ctggtcagct gtcagccgtt gaccctccgg tacaggcatg gcagtcatga acttgtagct
     7561 atcctaagaa cttgaagggc gcttagctgt gacacagcta aatcaagtat ggtgacgtga
     7621 cctatatctg gactggtaaa cgatggggat acccgcttac gcgagagcaa taagtggctg
     7681 gccaccattc tcgatacaaa actctgctga atagcagcga ttttcccagc gtggtgcgtc
     7741 ttttgcattt tactggttct gagttatcaa ttatctgttc actaatgact actctaaaaa
     7801 gttgtatcaa tttaagtgat atagggagtc cggagattca ccctttttgg tgatgaagtt
     7861 aacaataccg ccaattgcct gtgaaccgta cagtacggaa tatggacctt tcactacctc
     7921 aacacgctcc agcgcagact catctatcaa cagtcccaca ccataattat ctccggcgcg
     7981 ctgataagtt acctcctgac catcaatgag aattaaaaca cgggaggatg cttcgccacg
     8041 aatgcggatt tgtttacggc ctgccaagga gttgtctgtt atctctactc cggggatgtc
     8101 ctgcaaatta tcggctatgg aggcattggt ggagttctgc aatgtttgtt ggtcaataac
     8161 ctgtatagtc gccgggcttt cccaaaggtt gcttgccgaa cgattcgtgg tcacgacgag
     8221 cgtctcttcg ttgctgattt ttgtttctga agaagttgcg gttgcagtag aaactatccc
     8281 agaaatgcat aatgacagag tggaaaggat ataaattcga caaggta
//
LOCUS       DAZFXT010000181         8308 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.181, whole genome shotgun sequence.
ACCESSION   DAZFXT010000181 DAZFXT010000000
VERSION     DAZFXT010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8308
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.181"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..139
                     /locus_tag="ACHKW0_004510"
     CDS             <1..139
                     /locus_tag="ACHKW0_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477198.1"
                     /translation="WRQSLRGAGFRNLALALNTCYIEEVLASCSELGFRTDKMKNLTQ
                     I"
     gene            complement(260..1027)
                     /gene="yafV"
                     /locus_tag="ACHKW0_004511"
     CDS             complement(260..1027)
                     /gene="yafV"
                     /locus_tag="ACHKW0_004511"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HFO7477199.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMAVLREYRDKFPAWRDADEFRL"
     gene            1208..1654
                     /gene="ivy"
                     /locus_tag="ACHKW0_004512"
     CDS             1208..1654
                     /gene="ivy"
                     /locus_tag="ACHKW0_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HFO7477200.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(1697..4141)
                     /gene="fadE"
                     /locus_tag="ACHKW0_004513"
     CDS             complement(1697..4141)
                     /gene="fadE"
                     /locus_tag="ACHKW0_004513"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0033539 - fatty acid beta-oxidation using
                     acyl-CoA dehydrogenase [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HFO7477201.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            4381..4959
                     /gene="lpcA"
                     /locus_tag="ACHKW0_004514"
     CDS             4381..4959
                     /gene="lpcA"
                     /locus_tag="ACHKW0_004514"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HFO7477202.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            5165..5932
                     /gene="yafJ"
                     /locus_tag="ACHKW0_004515"
     CDS             5165..5932
                     /gene="yafJ"
                     /locus_tag="ACHKW0_004515"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006541 - glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HFO7477203.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(5903..6643)
                     /gene="dpaA"
                     /locus_tag="ACHKW0_004516"
     CDS             complement(5903..6643)
                     /gene="dpaA"
                     /locus_tag="ACHKW0_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan meso-diaminopimelic acid protein
                     amidase"
                     /protein_id="HFO7477204.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(6799..7077)
                     /gene="yafQ"
                     /locus_tag="ACHKW0_004517"
     CDS             complement(6799..7077)
                     /gene="yafQ"
                     /locus_tag="ACHKW0_004517"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HFO7477205.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(7080..7340)
                     /gene="dinJ"
                     /locus_tag="ACHKW0_004518"
     CDS             complement(7080..7340)
                     /gene="dinJ"
                     /locus_tag="ACHKW0_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HFO7477206.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
     gene            7526..8299
                     /gene="yafL"
                     /locus_tag="ACHKW0_004519"
     CDS             7526..8299
                     /gene="yafL"
                     /locus_tag="ACHKW0_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7477207.1"
                     /translation="MHDQPLLSMSLPSIPSFVLSGLLLLCLPFSSFASATTSHITFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAESRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGEIIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT         2033 a         2199 c         2079 g         1997 t
ORIGIN      
        1 ctggcgtcag tccttgcggg gagcgggctt tcgtaatctt gccctggcgt taaacacatg
       61 ttatatagaa gaagtgcttg catcctgctc agagcttggg tttcgaactg acaaaatgaa
      121 aaatttaaca caaatttaat tcatgctctt gccctgcggg gggattatta aatcttccca
      181 gttttttatt atttaactct cgctcagcac actaaacatc cctttctgaa ttccccgccc
      241 taaatacagc ccccactcac tacaacctaa actcatccgc atcccgccat gccggaaact
      301 tatcgcgata ttcccttaac accgccatcg acagctcggc atcaatgcgc gttgcctgat
      361 gcgcgtcggc agtagcgata atctcgcctt gtggattaat cacccggctg tcaccgcgat
      421 aatggcagcc gttgccatcg ctgccgacgc gattgcatcc cgccacatac gcctgattct
      481 caatcgcgcg ggccgtcagc aatgcctgcc agtgcagaga gcgcggagca ggccagttgg
      541 cgacgtacag ggcgaggtca taatcgttga gattgcgcga ccacacagga aaacgtaagt
      601 cgtagcacac cagcggcaaa atacgccagc cgcgccattc cacaatcact cgcgcattgc
      661 ccgctttata atgtagatgc tcatctgcca tgcggaacag atgacgctta tcataaaaat
      721 gtaccgtgcc gcccggctca accagcaaaa agcggttaac cgaaccagac tccgtttgta
      781 atgcaacact gcctgcaatc agcgcattgc actgctgcgc cttagctgtc atccagttca
      841 ctacgtcatc ttgtgctagc gacgaagctg ccgcttccat ggcaaagccg ctggtaaaca
      901 tctccggtag aacgatcaca tcgcgcccgg taataccttc cagttgacga tcaaaatgac
      961 gcaggttggc agggccatcc atccacacca gtggttgctg caaaagcgta atcttcaaac
     1021 caggcacggt gtacaactcc tttatgcgaa gggttttata actttaacac cttatcaggc
     1081 agttgcctta gcgcagaata aattgataac aaatgctgat attggaaata tctgatttgc
     1141 aaattatcgt gttatcgcca ggctttagaa ggttaataac atgggcagga taagctcggg
     1201 aggaatgatg tttaaggcaa taacgacagt cgccgctctg gtcatcgcca ccagtgcaat
     1261 ggcgcaggat gatttaacca ttagcagcct tgcaaagggc gaaaccacca aagctgcatt
     1321 taatcagatg gtacaagggc ataagctgcc tgcctgggtg atgaaaggcg gtacttatac
     1381 tcccgcacaa accgtaacgt tgggagatga gacgtatcag gtgatgagcg cgtgcaaacc
     1441 gcatgactgt ggctcgcaac gtatcgctgt gatgtggtcc gagaaatcta atcagatgac
     1501 ggggctgttc tcgactattg atgagaaaac gtcgcaagag aaactcacct ggctgaatgt
     1561 gaacgatgcg ctttcgattg atggtaaaac ggtgctgttc gcggcgttga ccggcagcct
     1621 ggaaaaccat ccggatggct ttaattttaa ataattagcg gataaagaaa cggagccttt
     1681 cggctccgtt attcaattac gcggcttcaa ctttccgcac tttctccggc aactttaccg
     1741 gcttcgtcgc cagctcttcc ggatcaaagt catcaacgtt aatactgcgc agacggcttt
     1801 cttcagcttt caccagaata gcggcttcat ctttatcaat cagccccttc gccagcgcgt
     1861 tgtgcgccag ttcatccaga cgggtaaacg gcaggttttt acccagctct ttacagatcc
     1921 gctgatgaat tgggtcggcg gcaatcacat ccaccagcgc ctcttccagc aagccaaccg
     1981 gattatgctc gctcggcgtc aggtactgac cgcgaccaat gcgggaacgg gtggcgttcg
     2041 gcacttgtaa aatcttcgcc actttatgat ccagcttgtc agaaggtgcc agataatgac
     2101 gtccggtcgg gaagatcacc acattcagca gcccggcaac cacgcggttc gggaagtttt
     2161 gcagtaaatc atccatcgcc tgttcagcct gatacagcgc atcttgtacg ccccagtgca
     2221 ccagcggcag gtcggcttca ttacggcctt cgtcgtcata acgcttcaga acggcagagg
     2281 cgaggtaaag ctggcttaaa atatccccca gacgtgccga gatgcgctca cgacgtttca
     2341 ggctgccgcc cagcacagcc atcgagacat cagaaagcag ggcgaggttg gcgctcaggc
     2401 ggttcaggtg ctgatagtag cgtttagtgg catcgccggt tggcgtgctg ctggttaaac
     2461 cgcgcgtcag gcccagccag aagctgcgaa ctttgttgct accgacgtga ccgatatgtt
     2521 tgaacaacag tttatcgaac gcgttgacgt cattgttctt cgctgcttcc atctcttcca
     2581 gcacgtacgg atggcaacga atcgctcctt gtccgaagat catcatgctg cgggtcagaa
     2641 tgttggcccc ttcaacggtg atggcaatcg gtgcgccctg gtaagcacgc gccaggaagt
     2701 tgctctgccc gagcataatg cctttgccac cagtgatatc catcgcatca ataatcgact
     2761 gctgcccgcg gtgggtacag tgatacttaa cgatagccga cagcacggca ggtttttcgc
     2821 cgagcataat gccgtaggta atcagcgatg ccgcagcatc catcacgtag gcattaccgg
     2881 caatacgcgc cagcggctct tcaatccctt ccatcttacc gatagagatt ttgaactgac
     2941 ggcgaatgtg agcatacgcg cccgttgcca gcgctaccga tttcacgccg ccggttgagt
     3001 tggaaggcag ggtgatgccg cggcctaccg agaggcactc caccagcatc cgccagcctt
     3061 gcccggccat tttcggccca ccgatgatgt aatcgatcgg tacgaagaca tctttaccgc
     3121 gcgtcggtcc gttctggaac ggtacgttca gcgggaagtg gcgacgacca atttccacgc
     3181 ccggcgtggt ggtcgggatc agcgcacagg taatacctaa atcttctgcg ccacccagca
     3241 gcttttccgg gtcggagagt ttaaacgcca gcccaagcac agtcgcaatc ggtgccagcg
     3301 taatgtagcg tttgttccag gtcagacgca tccccagcac ctgctggccc tgccattcgc
     3361 ccatgcagac aatcccggtg tccggaatcg cgcccgcatc ggaacccgct tccgggctgg
     3421 tcagtgcaaa gcaggggatc tcctgaccac gcgccagacg cggcagatag tgatctttct
     3481 gctcgtcagt gccgtaatgt tgcaacagtt cgcccgggcc taatgagttt ggcacgccga
     3541 cggtaatcgc caggatcccg ctcacgccgg agagtttttg cagcacgcga gactgggcat
     3601 aagccgagaa ctccagcccg ccgtactctt ttttgatgat catcgcgaag aaacgatgct
     3661 ctttaaggta cgcccacaac tccggcggca gatccgccag ctcatgggtg atctggaaat
     3721 cattcgccat ccggcaggct tcttctaccg ggccgtcgag aaacgcttgc tcttcggcgg
     3781 tcaggcgcgg ctgcggatag ttatgcagct ttttccagtc cggcttgccc tggaacaagt
     3841 cgccctccca ccaggtggtg cccgcatcaa tcgcttcttt ctcagtgcgc gacatcggcg
     3901 gcatcacctt acggaaaccg cgaaataccg gcgcggaaat catcgactta cgcataggcg
     3961 caaagttaaa tggcacgagg ataatggcca gaggcaccag tacccacgcc gaccacagac
     4021 cagcaacgcc gagggcggct gtccaggcga gcaaaatcag actgctgata aataagctca
     4081 cgcggtgata gaacaacgcg ccgagcagga caaccgtagc gagaatactc aaaatcatca
     4141 taacgaaaag ccccttactt gtaggaggtc tgaccacttg tgatgatatg gttgtagtgg
     4201 atgtaaaaac atttagcaat gtgtttacaa tataattaca acaaagctca cattgttgct
     4261 gtttttatcc gcacttcagg tcaaaaagtc ctggtcatag cacctgcccg tacttctcgc
     4321 ttttggcggt atccggtaca ctgcattttg tctattacat ttatgctgaa ggatatcctc
     4381 atgtaccagg atcttattcg taacgaactg aacgaagcgg cggaaacgct ggctaacttt
     4441 ttaaaagatg acgccaatat tcacgccatt cagcgcgcgg cggtcctgtt agcagacagc
     4501 tttaaagccg gtggcaaagt gctttcctgc ggcaacggcg gttcccattg cgacgctatg
     4561 cactttgccg aagagttgac cggtcgctac cgtgaaaacc gtccgggcta cccggcgatt
     4621 gctatttctg acgttagtca tatttcctgc gtcggtaatg atttcggttt caatgatatt
     4681 ttctcccgct acgttgaagc ggtaggtcgc gaaggcgatg tactgctggg gatctccacc
     4741 tccggtaact ctgcaaacgt gatcaaagcg atcgcagcgg cgcgtgagaa ggggatgaaa
     4801 gtgatcaccc tgaccggtaa agacggcggc aaaatggctg gcacggcgga tatcgaaatt
     4861 cgcgtaccgc actttggtta tgccgaccgc attcaggaga ttcacattaa agtgatccat
     4921 atcctgatcc agttaattga aaaagagatg gttaagtaag tctggcgtag gctggataag
     4981 gcgtttacgc cgcatccggc acttgtgcgc tgatgcctga tgcgatgctg acgcgtctta
     5041 tcatgcctac aaatctgtac ccgaaccgta ggccgaataa ttcgtttacg ccgcatccga
     5101 cctgaaaatt cttaaatcaa tcttcgccgg gggccattcg ctcccgctgt tgtggaggtt
     5161 acccatgtgc gaattgctcg ggatgagcgc caacgtccct accgatatct gctttagttt
     5221 caccggactt gtacagcgtg gtggtggaac cgggccacat aaagatggct ggggcattac
     5281 cttttacgaa ggtaaaggct gtcgcacatt taaagatcca caacccagct ttaattcccc
     5341 catcgccaaa cttgtccagg actacccgat aaaatcctgt tcggtggtgg ctcatattcg
     5401 ccaggctaat cggggcgagg tggcgctgga aaatactcac ccatttaccc gcgagttatg
     5461 ggggcgtaac tggacttatg cccataacgg acaactgacg ggctacaaat cactggaaac
     5521 cggcaacttc cgcccggtcg gtgaaaccga tagcgaaaaa gccttctgct ggctcctgca
     5581 taaattaacg cagcgttacc cgcgcacacc gggcaacatg gcggcagtgt ttaaatatat
     5641 cgcctcactg gcggatgaac tgcggcagaa gggcgttttc aacatgctgc tttctgacgg
     5701 gcgctatgtg atggcgtatt gctcgactaa tttacactgg atcacccgcc gcgcgccgtt
     5761 tggcgtggca acgctgctgg atcaggatgt ggaaatcgac ttcagctcgc agaccacacc
     5821 gaatgatgtg gtcacggtga ttgcaacaca gccgctgacg ggcaatgaaa cctggcaaaa
     5881 gattatgcca ggcgaatggc gcttattttg cctcggggag cgtgtagttt gatgccagtt
     5941 gtggctgcac aacttcgtgg cttaacggct tgctgaccac gtaacggcca ttgaccacag
     6001 aaacggtcgg tggcttacgg gtttgctcaa agtagtcgta gcccggcttc agctgctccc
     6061 agaagtcctt aaagttggaa tatttatggc gcttcatatt ggcgtcggtc atgcggaacg
     6121 ggtaaatact cacttgcacg ctcggctgac caaacaccag cgcaccagta acgaactgga
     6181 agatctcatc aataccctga ttggtcattg cgtagcagcc gatggaaaca caatcgccgt
     6241 ggatcatcag gtatttccct tcataaccat gcgcacggtc ataggcattg gggaaaccaa
     6301 tattaatcgc tttgtagtaa cggctgtctg gttttaactg attacgctgg acgctataaa
     6361 acccttccgg gcttttgaaa tcgccctgac gctgttttgg ccctaagccg ccggaatatt
     6421 tacagatttt ataactgtcg agcagctgat attgctcgcc cattttgacg tagagatcga
     6481 gcgtacgttc ttccttgaag atctggatgt agacagggga tcccatcaac tgctgcttat
     6541 actctttgct cactggcgtg gtcgagctac tgctgcccag caaaccggca aacgaaacgc
     6601 acgggatcaa caacatcgca agaattaatg cgattttacg catactgctt attccttgat
     6661 aaaacggtta cacacgccag gacggcaaaa tagatcccaa atcggaatag tctggatttg
     6721 gaaggctcac attatcacca aaagagtttt acgcaagcct gtcggcgcag ggtttacaaa
     6781 tttcatcggc agcgtgcttt acccaaagag cgccgcgtga gttccagttc tctcaaatcg
     6841 taaaagttta tcggtaagtt tgtaaatcag gatccagtcc ggttcgacat gagcatcgcg
     6901 ataacctttc catgaacctt gcagcgggtg gtctttataa acagctggaa gcggtaaagc
     6961 attattgata agaagcgtca taagatattt caatttattc atatccttat gacgcttttg
     7021 tgcaagtttt acatcctttg aaaattgtcc cgagtattca atatcccttt gaatcatatt
     7081 taaattccta atttatcaaa taaatcatcg gcgtctttgg ccttatgaac atcaacgcca
     7141 gcttcgctgt ttttgattga ttgaatggtt aattgattag gctcgcgtaa atcaaacggc
     7201 aatgcctttt cacgcgcgac ctttgtgagg gttatgcgaa ccaggtcaga gatggtcagc
     7261 cccatcccgg ccagtacgtc cgccgcctga ttcttcagat cttcatcgat tcgggcgcga
     7321 acaaatgcgt tagcagccat tggggtatct cctgtgctgt atatttattc agcttgaatt
     7381 gtagctcaaa tgagcaacag cgacaatcca tgagtaattt aataaacgga tgtctttaca
     7441 taattaactg aaatatattt attaatttta taataatggt ttattaagtt tgttcaggta
     7501 cgggccggga tacactgcaa catcaatgca tgatcagcca ttattgagta tgtccttgcc
     7561 gtcgattccc tcttttgtgt tgtcgggatt actgttgctt tgcttgccgt tttcttcatt
     7621 tgccagcgcc accacatcac atatcacttt cagctacgcc gcccgccagc ggatgcaaaa
     7681 tcgtgcgcgt ttattaaaac agtaccaaac ccatctgaaa aaacaggcca gctatattgt
     7741 ggaaggcaat gccgaaagca gaagggcgct acgccagcac aaccgcgaac agataaaaca
     7801 gcatccagaa tggtttcctg cgccgctcaa ggcgagtgac agacgctggc aggcgctggc
     7861 ggaaaacaac catttcttaa gcagcgacca tctgcataac atcaccgaag tggcgattca
     7921 ccgtctggag caacagcttg gcaagcctta cgtctggggc ggtacgcggc ccgatcaagg
     7981 cttcgactgt agcgggttag ttttttatgc ctacaacaag atccttgagg cgaaactccc
     8041 acgcacggct aatgagatgt atcactatcg ccgggcaacc attgtggcga ataacgacct
     8101 gcgccgggga gatttgctat ttttccatat ccacagccgc gagattgccg accatatggg
     8161 ggtgtatctg ggcgacggtc agtttattga gtcaccgcgc accggggaaa tcattcgggt
     8221 aagtcggtta gctgaacctt tctggcagga tcacttttta ggcgcgcggc ggatattgac
     8281 ggaagagacg attttgtaaa tgggtaat
//
LOCUS       DAZFXT010000182         8082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.182, whole genome shotgun sequence.
ACCESSION   DAZFXT010000182 DAZFXT010000000
VERSION     DAZFXT010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.182"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(9..476)
                     /gene="slyB"
                     /locus_tag="ACHKW0_004520"
     CDS             complement(9..476)
                     /gene="slyB"
                     /locus_tag="ACHKW0_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HFO7477208.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            750..1859
                     /gene="anmK"
                     /locus_tag="ACHKW0_004521"
     CDS             750..1859
                     /gene="anmK"
                     /locus_tag="ACHKW0_004521"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HFO7477209.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            1957..2286
                     /gene="mliC"
                     /locus_tag="ACHKW0_004522"
     CDS             1957..2286
                     /gene="mliC"
                     /locus_tag="ACHKW0_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HFO7477210.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2345..3001
                     /gene="pdxH"
                     /locus_tag="ACHKW0_004523"
     CDS             2345..3001
                     /gene="pdxH"
                     /locus_tag="ACHKW0_004523"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HFO7477211.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQLENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3130..4404
                     /gene="tyrS"
                     /locus_tag="ACHKW0_004524"
     CDS             3130..4404
                     /gene="tyrS"
                     /locus_tag="ACHKW0_004524"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HFO7477212.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            4463..5326
                     /gene="pdxY"
                     /locus_tag="ACHKW0_004525"
     CDS             4463..5326
                     /gene="pdxY"
                     /locus_tag="ACHKW0_004525"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HFO7477213.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5370..5975)
                     /gene="gstA"
                     /locus_tag="ACHKW0_004526"
     CDS             complement(5370..5975)
                     /gene="gstA"
                     /locus_tag="ACHKW0_004526"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HFO7477214.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     VVNPKGQVPAVLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6081..7583)
                     /gene="dtpA"
                     /locus_tag="ACHKW0_004527"
     CDS             complement(6081..7583)
                     /gene="dtpA"
                     /locus_tag="ACHKW0_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HFO7477215.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
BASE COUNT         2082 a         1990 c         2049 g         1961 t
ORIGIN      
        1 aaacttattt agcgcggaga aacggtcacc tgactgccat tgctggccag tacgacacgt
       61 tggcccggag agaaacgagt gttgccttgt ttctgtacca ccatgatggt attaccatcg
      121 tctttacgaa tttccagctc gacaccctgc gttttgttca ttgcactctg tacgccctga
      181 ccagctacgc cacctgcaac agcgcctgct gcagtagcca gagaacgccc ggttccgcca
      241 ccaacagtat tccccaggaa accaccaaga acagcaccgc caattgcacc gataacgttg
      301 gaatcatcac cgccctgaat ctgtaccgga cgtacgttaa cgatggtgcc atagctgaca
      361 ttctgtactt gtttcgcttc agaagcggta taaacatccc ctgacagggt gtcgttatta
      421 acacaaccga caagagacag acctaccatt gaaacaacca atacgcgttt aatcattgaa
      481 aaatctcctg ttcaccatga aacgccacgc aagcatccct catggtcaaa gtatatggca
      541 tatgtgaatc catggttaca tgttctacca aatatgagca aatcataatg aaagtaggat
      601 caaccaatta taaacaaaac attcatgatg attattgatt tgaagataat gttttactac
      661 caacggtaaa aatcggtcat tccgcatggt attcacgaaa agtttatgtt tgagtggtgc
      721 gcaaacgtca gattgaaaaa ggatgaacta tgaaatcggg ccgctttatt ggcgttatgt
      781 caggcaccag ccttgatggt gttgatgttg tgttggcgac aatagatgaa caccgggtcg
      841 cacagctggc aagcttgagc tggccgatcc cggtgtctct gaaacaggct gtactggata
      901 tttgccaggg ccagcagctt acactttcgc agtttggaca gcttgatact caactcgggc
      961 gactttttgc tgatgcggtc aatgccttgc ttaaggaaca aaacctgcag gcgagagata
     1021 tagttgcgat cggctgtcac ggtcaaaccg tctggcatga accgacgggc gtggcaccac
     1081 acactttaca gattggcgat aacaatcaaa ttgtggcacg caccggaatt acggttgtcg
     1141 gtgattttcg ccgtcgcgat attgccttgg gaggacaagg cgcaccgctg gtacctgcgt
     1201 tccatcatgc cttgctggct cactcaaccg agcgacgaat ggtgctcaat attggcggca
     1261 tcgccaatct gtcactgctc attcctgggc agccggttgg cggctacgat accggtcctg
     1321 gtaacatgct gatggatgcc tggatctggc gtcaggccgg taaaccttac gataaagatg
     1381 ccgagtgggc acgggcgggt aaagttattc tcccactgct gcaaaatatg ctcagcgacc
     1441 catatttctc gcaacctgca ccgaaaagca ccggacgcga atactttaac tacggctggc
     1501 tggagcgcca tttacgccat tttccgggtg ttgatccccg tgatgttcag gccacactgg
     1561 cagaactcac cgccgtgacc atttctgaac aagttttgtt gagcggtggc tgcgaacgat
     1621 tgatggtatg tggtggaggt agtcgtaatc cgctactcat ggcgcgtctg gcggcattac
     1681 tgccaggcac agaagtcacc accaccgatg ccgttggcat tagtggcgat gacatggaag
     1741 cattggcttt cgcctggctt gcctggcgga cgctggcggg attaccagga aatctgcctt
     1801 ccgtcactgg cgcaagccag gagacggtac tgggggctat tttccctgct aacccgtgat
     1861 atcagagtta actgaatttt tcttaccgcg tctgccgtta cacttggaac gttagggagg
     1921 gcgtattgcc ctccagacca ggaaagtctt cgggaaatgt ctatgaaaaa actgttactg
     1981 atctgtttgc cggtgcttct caccggctgt agcgcgttca atcaactggt tgagcacatg
     2041 caaaccgcta cgctggaata ccagtgtgat gaaaaaccgt tgacggtcaa actgaataat
     2101 ccgcgccagg aagtcagttt tgtttacgat aatcaactac tgcatctcaa acagggcatt
     2161 tcagcgtctg gtgcacgtta cactgacgga atctatgttt tctggtcgaa aggcgatgaa
     2221 gcgactgtct ataaacgcga ccgcatcgta ttgaataact gtcagttaca aaatccacag
     2281 cgttgagatt tttccagggg cggcgcacaa tagcgtcacc cactgacaat ccgtaaagaa
     2341 aaccatgtct gataacgacg aattgcagca aatcgcgcat ctgcgccgtg aatacaccaa
     2401 aggcgggtta cgccgtcgcg atcttcccgc cgatccatta accctttttg aacgttggct
     2461 ctctcaggcg tgtgaagcca aactggcaga ccctaccgcg atggtggtcg ctaccgtgga
     2521 tgaacatgct cagccttatc aacgcatcgt tttactcaaa cattacgacg aaaaaggcat
     2581 ggtgttttac accaacctcg gtagccgtaa agcacatcaa cttgaaaata atccgcgcgt
     2641 tagcctgctg ttcccgtggc atacccttga acgccaggtg atggtgatcg gtaaagcaga
     2701 acgactttcg actctcgaag tgatgaaata ttttcatagc cgcccgcgtg atagccagat
     2761 tggtgcatgg gtttcgaagc agtccagtcg catttctgcc cgcggtatcc ttgaaagtaa
     2821 attcctggag ctgaagcaga agtttcaaca gggcgaagtg ccattgccga gcttttgggg
     2881 cggttttcgc gtcagccttg aacagattga gttctggcag ggcggtgagc atcgcctgca
     2941 tgaccgcttt ttgtaccagc gagaaaatga tgcgtggaag attgatcgtc ttgcaccctg
     3001 aaaagatgca aaaatcttgc tttaatcgct ggtactcctg attctggcac tttattctat
     3061 gtctctttcg catctggcga aaagtcgtgt accggcaaag gtgcagtcgt tatatacatg
     3121 gagattttga tggcaagcag taacttgatt aaacaattgc aagagcgggg gctggtagcc
     3181 caggtgacgg acgaggaagc gttagcagag cgactggcgc aaggcccgat cgcgctctat
     3241 tgcggcttcg atcctaccgc tgacagcttg catttggggc atcttgttcc attgttatgc
     3301 ctgaaacgct tccagcaggc gggccacaag ccggttgcgc tggtaggcgg cgcgacgggt
     3361 ctgattggcg acccgagctt caaagctgcc gagcgtaagc tgaacaccga agaaactgtt
     3421 caggagtggg tggacaaaat ccgtaagcag gttgccccgt tcctcgattt cgactgtgga
     3481 gaaaactctg ctatcgcggc gaacaactat gactggttcg gcaatatgaa tgtgctgacc
     3541 ttcctgcgcg atattggcaa acacttctcc gttaaccaga tgatcaacaa agaagcggtt
     3601 aagcagcgtc tcaaccgtga agatcagggg atttcgttca ctgagttttc ctacaacctg
     3661 ttgcagggtt atgacttcgc ctgtctgaac aaacagtacg gtgtggtgct gcaaattggt
     3721 ggttctgacc agtggggtaa catcacttct ggtatcgacc tgacccgtcg tctgcatcag
     3781 aatcaggtgt ttggcctgac cgttccgctg atcactaaag cagatggcac caaatttggt
     3841 aaaactgaag gcggcgcagt ctggttggat ccgaagaaaa ccagcccgta caaattctac
     3901 cagttctgga tcaacactgc ggatgccgac gtttaccgct tcctgaagtt cttcaccttt
     3961 atgagcattg aagagatcaa cgccctggaa gaagaagata aaaacagcgg taaagcaccg
     4021 cgcgcccagt atgtactggc ggagcaggtg actcgtctgg ttcacggtga agaaggttta
     4081 caggcggcaa aacgtattac cgaatgcctg ttcagcggtt ctttgagtgc gctgagtgaa
     4141 gcggacttcg aacagctggc gcaggacggc gtaccgatgg ttgagatgga aaagggcgca
     4201 gacctgatgc aggcgctggt cgattctgaa ctgcaacctt cccgtggtca ggcgcgtaaa
     4261 actatcgcct ccaatgccat caccattaac ggtgaaaaac agtccgatcc tgaatacttc
     4321 tttaaagaag aagatcgtct gtttggtcgt tttaccttac tgcgtcgcgg caaaaagaat
     4381 tactgtctga tttgctggaa ataatgcatt aagtggaaag ggggagtgag aaatcactcc
     4441 ccctggtttt tatacaggga acatgatgaa aaatattctc gctatccagt ctcacgttgt
     4501 ttatggtcat gcgggtaaca gtgcggcaga gtttcctatg cgccgcctgg gcgcgaacgt
     4561 ctggccgctg aacaccgttc aattttctaa tcacacccaa tacggcaaat ggactggctg
     4621 cgtgatgccg cccagccatt taaccgaaat tgtgcaaggc attgccgcca ttgataaatt
     4681 acacacctgt gatgccgtgt taagtggcta tctgggatcg gcggagcagg gtgaacatat
     4741 cctcggtatc gtccgtcagg tgaaagccgc gaatccgcag gcgaaatatt tttgcgatcc
     4801 ggtaatgggt catccggaaa aaggctgtat cgttgcaccg ggtgtcgcag agtttcatgt
     4861 gcggcacggt ttgcctgcca gcgatatcat tgcgccgaat ctggttgagc tggaaatact
     4921 ctgtgagcat gcggtaaata acgtcgaaga agcggttctg gcagcgcgcg aactcattgc
     4981 gcaagggcca caaattgtgt tggttaaaca cctggcgcga gctggctaca gccgtgaccg
     5041 ttttgaaatg ctgctggtca ccgccgatga agcctggcat atcagccgtc cgctggtgga
     5101 ttttggtatg cgccagccgg taggtgttgg tgatgtgacg agcggtttac tgctggtgaa
     5161 actgcttcag ggggcaacgc tgcaggaggc gctggaacat gtgaccgctg cagtctacga
     5221 aatcatggtg accaccaaag caatgcagga atatgagctg caagtggtgg ctgctcagga
     5281 tcgtattgcc aaaccagaac attacttcag cgcaacaaag ctctgaaata tctcatttcg
     5341 gcccacaacg acctgtgggc cgatggatat tactttaagc cttccgctga cagcgcgtct
     5401 tgtacttccg gacgttcagc catacgttgc ataaatgctg caatgtgctc taacccttcc
     5461 agattcagtt tcaccgcgta tgcccagcgc agaaccgtaa acagataggc atcagcaatt
     5521 gtaaatcttt gcccgcagat ccagtgctca tccttcagtg cctcgttcac atattgcagc
     5581 ttcttctcca gctgcgcgcg aactgtcggt ttgtactctt ccggtgtatc agggcgaaac
     5641 agaggtgtga aacctttatg cagctcggtg gcgatgtaat tcagccattc gatggtttta
     5701 tagcgggaaa tactgtttac cggtgccagc aactggcggt cggggacgct gtcggcaaga
     5761 tactgcataa tcgctacgcc ttccgtcagc aaagtaccgt catccagcag cactgcaggc
     5821 acctgcccct tagggttaac gacaaagtaa tcgtcaccgt tttcgagacg ttttttcatt
     5881 aaatccacac tgacgagggt aaaatccttt ccgctctcac gcagggtgat atgggaagcg
     5941 agagagcagg cacccggttt gtagaacaat ttcatcggta actccttttg tctgtggttt
     6001 aaagtatggt agtgcgttgc tgaacaaaaa aaaagccgct aacgaaaagt tagcggcttg
     6061 taaaagagtt tccctgaatt ttacgctaca gcagctttcg ccgctttgtc tgcagcgtca
     6121 tcctgcgtca tacggtgcag tttaggcgcg gtcagcagca tcagtactgc aataatggca
     6181 gtagcgacac caatctgcaa gaatacgcga ccataaactt ccagtgacat cagcgggtcg
     6241 gtaacgttat ccggcacagc catcataccc gcaacataac caccaatcag gtttgcaccg
     6301 gcagtggtca ggaaccagct acccataatg aagcccatca gacgctgcgg aacgagttgt
     6361 gcaaccattg ccagacccag accagagatc atcagttccc cgatgctctg caggccatag
     6421 cttgcgacca gccagcttac agacacgata ccagcgtcag acgcgaattt cgcacccagc
     6481 ggcagaatca ggaacgcacc agaacacatc accatgccga ttgcaaactt ggttggcatc
     6541 ggcagggtat cgcccatctt gttatagata gcggccagaa tcggactacc gatgatgatc
     6601 cagaacgggt tcagtgcctg atactgctca ggttctacgg ccagacccag aatggagtgc
     6661 tcaacgttac gaatcgcaaa gaagttcagt gacgttggca tctggctgta cagcacgaag
     6721 aagataatgg cttcgagcat caggatgaag gcaacgatca ttttacgacg cgcagcacct
     6781 ttcatcgcga aggcttcttt accgaagata accacgatac cgaaggcaac aacgcccagc
     6841 gccatacgcg caacttcctg attgtgcagc agccaggtgg cgatagcgat cagtgccaca
     6901 acaccaataa tggtcagcag caggttacgg tagttgatag gctcgaagtc tggttttgaa
     6961 ccgtactgtt taacccagcg ttggcagaag gcgaagttaa cgatagtgat cagcaggcct
     7021 acaacgctca acgcaaacgc aacactccag ccgtatttcg cggccagcca cggcgtagca
     7081 atcatagaga agaaagagcc gatgttgacg gacatgtagt acatggtgaa tgcaccgtcc
     7141 agacgcgggt cgtttttctc atagcatgta gaaagcagag aagacgggtt agctttaaac
     7201 aggccgttac cgaccgcaat agccgccata cccatataaa cgataccggc gtcgtgacca
     7261 gaccaggcaa ccagagcata accaatcgcc agcacaatag cgccgagcat aattacgcgt
     7321 ttagtaccca gtaccttgtc acctaaccag ccgccgatag cgaccagacc ataaaccagg
     7381 gcactaaagg aagagaaaag ggtgattgaa tccgcttcag acatacccag ttgtttaacc
     7441 aggtaaacag ccataattcc ttgtaggccg taataaccaa aacgttccca taactcaatc
     7501 gagaagatga gatagaacgc cttcggttgt ttgaaagcgt tcaaactgac gctttcagtt
     7561 ggtttttggt ttgcagtgga cacttttacc tcttttttta catcccatat taacgggggt
     7621 gctttctcgc gttatgtcta aaaaacatac gcgcaattgt tatagtggga ggggaaacgg
     7681 caggtaatgt tcactatctt gcactttcag acaagtcctt tgtaataatc tgttacatat
     7741 aactgaggcg gggaattcaa ccattccggg aaatgttatc cagcgttaaa tttttcaaaa
     7801 aacgtaacgg cagatttttt cactgcactg agaggggggt gatggcttaa cggcgatatg
     7861 ttctgctatt tagcgttttt actagtgaga tagtccagtt tctgaaaaat agccagtgta
     7921 atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa atgattttaa cctgattctt
     7981 gcaatgtttt tacattgcga cagcatcgtg atcttgatca cgaatgaata gaaaatttcg
     8041 ctattaaaaa aacaattaac ctgcattttt ggttatccgc aa
//
LOCUS       DAZFXT010000183         8009 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.183, whole genome shotgun sequence.
ACCESSION   DAZFXT010000183 DAZFXT010000000
VERSION     DAZFXT010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8009)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8009)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8009
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.183"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..235
                     /locus_tag="ACHKW0_004528"
                     /pseudo
     CDS             1..235
                     /locus_tag="ACHKW0_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(316..474)
                     /locus_tag="ACHKW0_004529"
     CDS             complement(316..474)
                     /locus_tag="ACHKW0_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7477216.1"
                     /translation="MFYKLCLLAVVILLLVMVMMDFTSRIMLVLTDGALVCGIVVLLW
                     PMMKEQNE"
     gene            complement(560..1303)
                     /locus_tag="ACHKW0_004530"
     CDS             complement(560..1303)
                     /locus_tag="ACHKW0_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308843.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477217.1"
                     /translation="MLSSCIGVVMKDGALLRSSSLFIAYMGCLGWGSAYFYGWGTSFY
                     YGFPWWIVGAGVDDVARSLFFAVIVIAIFLIGWGIGVVFFFAVKRKHSMQELNVFRLY
                     FAVELLFVPAIIEFSILRQKIQVPLLLLSAAIALAVTISIRSYGRFLSVSCFYDKPFI
                     KKHFFEIVMIAFVAYFWLFSFLTGYYKPQFKKEYEMINYNDGWYYVLARYDNCLVLST
                     SFNAGSKRFVIYQSAQDKNLQVDIVRTRI"
     gene            complement(1488..2177)
                     /locus_tag="ACHKW0_004531"
     CDS             complement(1488..2177)
                     /locus_tag="ACHKW0_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage antitermination protein Q"
                     /protein_id="HFO7477218.1"
                     /translation="MNTQYLQYVREQLMAATADLNGATKGQLEAWQEHAQFDTGTYKR
                     KKPRILDVVTGKMITLDNTPTSGKQSYAKGSSIALVSPVEFSTSSWRRAVLSLDEHQK
                     AWLLWCYSESVRWGHQVTITQWAWSEFKDLLSNRKIAGKTLDRLKTLIWLAAQDVKSE
                     LAGREAYEYQTLASLVGVTTKNWSETFTERWVAMKHIFLQLDSDALLLVTRTRSKQKA
                     AFLQQNIAKLD"
     gene            complement(2192..2314)
                     /locus_tag="ACHKW0_004532"
     CDS             complement(2192..2314)
                     /locus_tag="ACHKW0_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000971054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HFO7477219.1"
                     /translation="MMSESYISELLRCRWGLLCLCRFPDSVLNDYRMLKNYAKI"
     gene            2652..3611
                     /locus_tag="ACHKW0_004533"
     CDS             2652..3611
                     /locus_tag="ACHKW0_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A deacylase LpxR family protein"
                     /protein_id="HFO7477220.1"
                     /translation="MKKSVIAGVFIALSFTTCSAIANSLALSLANDDAGKFQPILNDI
                     YGNKHENRDDYSQGLFLGYSHDISDSSQLSLHIAQDIYSPSGSNKRHNTAVTGDRAFS
                     AYTHTGIEWNSLANDWIRYRLGTDIGVVGPDAGGQKVQNKAHEIIGAEKYHAWDDQIE
                     NRYGYTVKGMLSMTPSMDILGANVGLYPEVSAVTGNLFQYVAYGATIAIGNDKTFNSD
                     NGFGLLAPRGLMHMSDTSGFKYKIFAGMERRDVNRNYTLEGKTIQTKQTTVSLNKTVD
                     EYQVGATIGYAPVAFTLAFNKVTSEFKTGDDYSFINGAITFFF"
     gene            complement(3755..3826)
                     /locus_tag="ACHKW0_004534"
                     /pseudo
     CDS             complement(3755..3826)
                     /locus_tag="ACHKW0_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            complement(3823..4488)
                     /locus_tag="ACHKW0_004535"
     CDS             complement(3823..4488)
                     /locus_tag="ACHKW0_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HFO7477221.1"
                     /translation="MNIYERIDGSKYRNIWVAGDLHGCYTNLMNKLDTIGFDTKKDLL
                     ISVGDLVDRGTENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKETLAKVLAHKAEELPLIIELVSKDKKYVICHADYPCDKYEFGKPVDHQQVIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGA"
     gene            complement(4485..5096)
                     /locus_tag="ACHKW0_004536"
     CDS             complement(4485..5096)
                     /locus_tag="ACHKW0_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7477222.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNAFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELISRIG
                     QEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            complement(5089..5259)
                     /locus_tag="ACHKW0_004537"
     CDS             complement(5089..5259)
                     /locus_tag="ACHKW0_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein NinF"
                     /protein_id="HFO7477223.1"
                     /translation="MIDPNRSYEQESVERALTCANCGQKLHVLEVHVCEHCCAELMSD
                     SNSSMHEEEDDG"
     gene            complement(5256..5438)
                     /locus_tag="ACHKW0_004538"
     CDS             complement(5256..5438)
                     /locus_tag="ACHKW0_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HFO7477224.1"
                     /translation="MRRQRRSFTDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRHRARNTR"
     gene            complement(5435..5962)
                     /locus_tag="ACHKW0_004539"
     CDS             complement(5435..5962)
                     /locus_tag="ACHKW0_004539"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7477225.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWISHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTIVS
                     KAALMAIGQGVRRAT"
     gene            complement(5959..6399)
                     /locus_tag="ACHKW0_004540"
     CDS             complement(5959..6399)
                     /locus_tag="ACHKW0_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HFO7477226.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPIVVTIQERNRSLD
                     QNRKLWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQPTS
                     RMRVSEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            complement(6473..6763)
                     /locus_tag="ACHKW0_004541"
     CDS             complement(6473..6763)
                     /locus_tag="ACHKW0_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ren"
                     /protein_id="HFO7477227.1"
                     /translation="MTGKEAIIHYLGTHNSFCAPDVAALTGATVTSINQAAAKMARAG
                     LLVIEGKVWRTVYYRFATREEREGKMSTNLIFKECRQSAAMKRVLAVYGVKR"
     gene            complement(6760..7461)
                     /locus_tag="ACHKW0_004542"
     CDS             complement(6760..7461)
                     /locus_tag="ACHKW0_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7477228.1"
                     /translation="MKNIAAQMVNFDREQMRRIANNMPEQYDEKPQVQQVAQIINGVF
                     SQLLATFPASLANRDQNELNEIRRQWVLAFRENGITTMEQVNAGMRVARRQNRPFLPS
                     PGQFVAWCREEASVIAGLPNVSELVDMVYEYCRKRGLYPDAESYPWKSNAHYWLVTNL
                     YQNMRANALTDAELRRKAADELSCMTARINRGEAIPEPVKQLPVMGGRPLNRAQALAK
                     IAEIKAKFGLKGASV"
     gene            complement(7458..>8009)
                     /locus_tag="ACHKW0_004543"
     CDS             complement(7458..>8009)
                     /locus_tag="ACHKW0_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Replication protein O"
                     /protein_id="HFO7477229.1"
                     /translation="IPQNEGKSPKTRDKTSLKLGDCYPSKQGNTKDTNTKENRKDYSS
                     ENSGESSDQPENDLSVVKPDAAIQSGSKWGTAEDLTAAEWMFDMVKTIAPSARKPNFA
                     GWANDIRLMRERDGRNHRDMCVLFRWACQDNFWSGNVLSPAKLRDKWTQLEINRNKQQ
                     AVVTASKPKLDLTNTDWIYGVDL"
BASE COUNT         2166 a         1968 c         1623 g         2252 t
ORIGIN      
        1 taatgtttaa tcgacatcga atttctcccg tgtagaggaa cagagttaaa aagccggaag
       61 cggaatcaaa tcacaggatg accatctgcc agtggcaggt cataaaaaaa aggctgcgca
      121 atgcgcagcc agaactcaca aggaaaatga taaaaggaat aacactagtg atgtacgcat
      181 ggcgcctccc gctaagttct gcaatgatca aacagaactc gctacgtgcc cttaaaactc
      241 gatcatttag cccctccaag gaggattcac catgcggttg attttttaat aaacagtaaa
      301 caaaaaagtc aagaattatt cattctgttc tttcatcatc ggccacagca ataccacaat
      361 gccgcagacc agagcgccat cagtcagtac caacattatc ctgctggtga aatccatcat
      421 caccatcact aaaagcagga tcacaacagc aagcagacac agtttataaa acaatgttca
      481 gaaaacgcat tcagcatgcc taaggttcta ttcctacgaa tagccaactt gcaacttaaa
      541 atattattta tgcagccaat taaattctgg tccttacaat atcaacctga agattcttat
      601 cttgtgctga ttgataaatg acaaaccttt tactacctgc attgaaagaa gtagacaaaa
      661 ccagacaatt atcataacga gcaagaacat aataccaacc atcattataa ttaatcattt
      721 catattcttt cttaaactgt ggtttgtaat atcctgtcag aaatgaaaaa agccaaaaat
      781 atgccacaaa agcaatcatc acaatctcaa aaaaatgttt ttttataaat ggcttatcat
      841 agaagcatga taccgataaa aatcgcccat aagatcttat cgaaattgta accgccagcg
      901 caatcgctgc tgacagtagc aaaagaggta cctgaatctt ctgtctcaat atagaaaact
      961 caataattgc cggcacaaac aataattcca cagcaaaata aaggcgaaat acatttagct
     1021 cttgcataga atgttttctt ttcactgcga aaaagaatac aacaccaata ccccaaccga
     1081 taagaaatat agcaatgacg ataactgcaa aaaataaact tctggcaaca tcatcaacac
     1141 ctgcacctac aatccaccat gggaagccgt agtaaaaaga agtaccccat ccatagaaat
     1201 aagcactccc ccatccaagg catcccatgt aggcaataaa aagtgaagaa ctcctgagca
     1261 gcgcaccatc cttcataacc accccaatac aagatgataa cattggctta caactcataa
     1321 caaaagcaat tcaatgccgt caagaggtta caggctaaaa aaactctatt acatagcagc
     1381 cagcatgttt accgtacaag tacaactcag ggcataaaaa aaacccactc ggcagcgggt
     1441 ttatacattt tttacaacat accaaatttg catgaagtat atggctttta atccagtttt
     1501 gcaatatttt gctgtaaaaa tgctgccttt tgttttgaac gtgttctcgt cacaagcaat
     1561 aaagcatcac tatcaagctg tagaaaaatg tgcttcattg caacccagcg ttcagtaaat
     1621 gtctcggacc agttttttgt tgtcactccc accaatgatg ccagtgtctg gtattcatag
     1681 gcctcacgcc ctgcaagttc gctcttcaca tcctgtgcag ccagccagat taacgtcttc
     1741 aggcgatcca gtgtcttacc tgcaattttt ctgttactta acaaatcttt aaactcgctc
     1801 catgcccatt gcgttatggt gacctgatgc ccccatcgaa cgctttcgct gtaacaccaa
     1861 agcaaccatg ctttctgatg ttcatcgaga gacaaaaccg cgcggcgcca tgaagaggtt
     1921 gagaattcaa ccgggctgac caaagcaatg gatgaacctt ttgcgtacga ctgcttaccg
     1981 gaagtcggcg tattatccag cgtaatcatc ttgccagtta ccacatccag aatgcgcggc
     2041 ttctttcgtt tgtatgtacc agtatcaaat tgtgcatgct cctgccaggc ttcgagctgg
     2101 cctttcgttg ctccgttcaa gtcagcagta gctgccataa gttgctcacg aacatactgt
     2161 aaatattggg tattcatgca gtaaatcctt tctatatttt ggcataattc ttcaacattc
     2221 ggtaatcgtt caaaaccgaa tcggggaaac gacataagca caggagcccc cagcgacagc
     2281 gaaggagttc tgatatataa gactcagaca tcattcattc cccggttctc caatatctgt
     2341 ttcactcatc atccataact acctgtaatt gccccccttt ttttgtaaca gttcttatat
     2401 tgctatatag aatagccatt actaatgcat ttaaatttaa taaaataaaa attataaaaa
     2461 acataaaaca ccacgtaaac acacttaata aaaacaccgt tacattaaaa gataataaaa
     2521 accgcaataa aaaaagaata aatcaattgt ctcacgcaat tataaaacat catattgatt
     2581 acgcaccttg tattacaaac tcatgtatgt aaaatacgcg caccattcaa aaaaaaggaa
     2641 gacaataaca tatgaaaaaa agtgtcatcg ctggcgtctt tattgctctg tcatttacca
     2701 cgtgttcagc tatcgcgaac agccttgcat tatcattagc aaatgatgat gcagggaagt
     2761 ttcaaccaat acttaatgat atttatggca ataaacatga aaacagagat gattactcac
     2821 aaggcttatt tctgggatat agccacgata tctcagactc gagccaatta tctctccata
     2881 ttgcgcaaga tatttactct ccatcaggca gtaataaaag acacaacaca gctgtaactg
     2941 gagacagagc ttttagtgca tacactcaca ctggtattga atggaactcc cttgcgaatg
     3001 actggattcg ctatcgatta ggtactgaca taggtgttgt tggccccgac gcaggcggtc
     3061 agaaagtaca aaataaagct catgagatta ttggggcaga aaaatatcat gcatgggatg
     3121 atcaaataga gaatcgctac ggttatactg taaaagggat gctatccatg acaccaagta
     3181 tggatatttt aggtgctaat gttggattat accctgaagt ttctgctgtt actggaaact
     3241 tatttcaata tgtagcatat ggcgcaacca ttgccattgg taatgataaa accttcaatt
     3301 cggataatgg ctttggtctg ctggctcccc gtggtttaat gcatatgtcc gatacaagcg
     3361 gattcaaata caagattttt gcaggtatgg aaagacgaga tgtcaatcgc aactatactc
     3421 tcgaaggaaa aacaatacag acgaaacaaa caacagtatc gctaaacaaa actgttgatg
     3481 aatatcaagt tggcgcaaca attgggtatg cacctgtagc cttcacacta gcatttaata
     3541 aagtaacatc agaattcaag acaggggatg actattcatt tataaatgga gcaatcacct
     3601 tcttttttta actgaattga attcaatcaa aataacataa gtccaacaaa aacataaagt
     3661 gcgaaatgaa tgccagctcc atttatttcg cactataaaa gattaaaagt tgcaataaaa
     3721 taataaaatg actcagttac gaaaaccaat aaactgtggc cagtagtgag tcgctcatca
     3781 tcgggctttt tggcgaatga aatttagcta cgctttcgag tctcatgcgc cttctccctg
     3841 tacctgaatc aatgtgaggt ttccgcagaa cactgcgcca gtatcgatat acatctggtt
     3901 ggcaaatttg agtggtttca ctgctggcgt atgaccaaag atgaacgtgt ccgcgccttt
     3961 gatttctttc acgatcccgt cttgtgagtt gctgattcgt tcgcggttcc agattacctg
     4021 ctgatgatca actggctttc caaactcgta tttatcacaa ggataatcgg cgtggcaaat
     4081 gacatatttt ttatctttgc tcaccagttc gatgattaac ggaagttctt ctgctttatg
     4141 ggcaagaact ttagccagag tctctttgtc gtaatcgaga ttaaagaacc agccaccgcc
     4201 attaagcagc cagtgattaa cgtttccacg ctctgataag ccatcaatca tcatttgctc
     4261 atggtttcca cgcacagctc tgaaccaggg gaatgtgatt aattccagac attcgacgtt
     4321 ctctgtaccg cgatcaacca aatcgccaac cgagatgagc aggtcttttt tggtgtcgaa
     4381 tccaatcgta tccagtttgt tcatcaggtt cgtgtagcat ccgtgcaggt cgccagctac
     4441 ccaaatattt cggtatttgc tgccatcaat tctttcgtag atattcatgc agcctcactt
     4501 ctgctgtttc gcaggtcttt gagtttctgt tggtactctg ccttgatcgc cttgcactct
     4561 tcgacagtcc agcgatggcg gttatggttt gattcgattt cgtctactgc ttcctgcccg
     4621 atgcggctaa tcagttcgac gcgatacgga acgagatttc cgcttttgtg ctggttgcac
     4681 accacgcatt gcttgtgaat attgcgttca tcaaatcgga gttgaggtgc cgcagcagtt
     4741 gtccggtaat gtccggcatc ccactgagca gacgtgagcg ttccgcacga gatacatggt
     4801 aagtcgcggt ctctttctct gatgaaggcg tttacggctt gttgggcttg tttaatccag
     4861 taactgcggg gctttaaggc gagttttcga atcttaagtt tatctttctg tttctgctcc
     4921 tctcgtcgtc gtttcttctc tgctgctttt tccgcttttt cgcgttcttt acttcgtcgt
     4981 tcgagtgcta tcttggttcc acactctgga gagcaccacc actgattagc gaatgcaggg
     5041 tgaaaccatt cccgacattc atcgttttta catcgtcttc gcgctggttt agccatcatc
     5101 ttcttcctcg tgcatcgagc tattcgaatc gctcatcagc tctgcacagc agtgctcaca
     5161 cacgtgaact tccagcacat gcagcttctg accgcaatta gcgcacgtta aagcccgctc
     5221 gacgctttct tgttcgtaac ttcgatttgg gtcaatcacc ttgtattcct cgcacgatgt
     5281 cttagccacc ggatatccca caggtgagcc gtgtagttga aggtttttac gtcagattct
     5341 tttgggattg gcttgcgttt atttctggag cgtttcgttg gaaggtattt gcagttttcg
     5401 cagatgatgt cggtgaaact tcgtcgttgt cgcctcatgt cgccctcctg acgccctgcc
     5461 cgatcgccat caatgccgct ttggatacga tagtaaacat ccgtcgagga ctaatgaacg
     5521 gtcgccaaat cagcagcatg gagcctttgc tgtttccctt cttctccagc cctgtcgatg
     5581 gttcgataaa attaatccgt ccatcagtga taatacgaac ttcgtcaaca ctctccagag
     5641 ccttgctgaa ccatccgaca gacatatcct ctggcacaag catcactacc gtctgtcgct
     5701 gttgtatgca ctgctcagcg gctttttcca cccacggcct gatattgctg tacggtgggt
     5761 tattccagat tgcaccgtgg cttatccact cagaatttag cgcgtcgtca gcctcagtta
     5821 gccagtgagc gcacagagcg tttttgtcgc tcgctgccga atccagccag aatccaaact
     5881 caatatccag tgcatcaaaa agccaaagcg gcgtttgcca gcagtccttg tcgtgtgctg
     5941 gcgtatttga tttgatagtc atgcagcccg atctccccat cgcgctttcc attcgagagc
     6001 cagtcgcgct tcgtctgacc acttaacgcc acgctctgta ccgaatgcct gtataagctc
     6061 taatagctcc gcaaattcgc ttacacgcat cctgctggtt ggctggccta ttaccacaaa
     6121 gccattcccg gcaaggttag gaacaacatc ctgctgcttt aatgctgcgg taaacacaca
     6181 cttccagctt tctgcatcca gccagcgacc atgccattca acctgacgag agacgtcacc
     6241 taagcaggcc catagcttcc tgttttggtc taagctgcgg ttgcgttcct gaatggttac
     6301 tacgattggt ttggttgggt ctggaaggat ttgctgtact gcgtgaatag cgttttgctg
     6361 atgtgctgga gatcgaattt caaaggttag ttttttcatg acttccctct cccccaaata
     6421 aaaaggcctg cgattaccag caggcctgtt attagctcag tgatgtagat ggtcatcttt
     6481 taactccata taccgccaat acccgtttca tcgcggcact ctggcgacac tccttaaaaa
     6541 tcaggttcgt gctcatcttt ccttcccgtt cttccctagt agcgaaccgg taatacaccg
     6601 ttcgccagac cttaccttcg ataaccagaa gacctgcccg tgccatttta gccgcggcct
     6661 gatttatgct ggttactgtt gcgcctgtta acgcggcaac gtccggcgca cagaagctat
     6721 tatgcgtccc caggtaatga ataattgcct ctttgcccgt catacacttg ctcctttcag
     6781 cccaaactta gctttgattt ctgcgatctt cgccagagcc tgtgcacgat ttagaggtct
     6841 accgcccatg acaggaagtt gttttactgg ttcaggtata gcctcaccac ggttaattcg
     6901 tgcggtcata caggacagtt catcggcagc cttgcgccgt aattccgcat cagtaagcgc
     6961 attggcccgc atgttctgat acaggttggt aaccagccag tagtgcgcgt ttgatttcca
     7021 cggataagac tccgcatccg gatacaggcc tcgcttccgg caatactcgt aaaccatatc
     7081 aaccagctcg ctgacgtttg gcagtccggc gataacggat gcttcttccc ggcaccatgc
     7141 aacaaactgc ccgggtgatg gcagaaatgg tcgattctgc cgacgggcta cgcgcattcc
     7201 tgcgttaacc tgttccattg tggtgatccc gttttcccgg aaagccagaa cccactggcg
     7261 gcggatttcg ttcagttcgt tctggtcacg gttagccagg ctcgccggga aagttgccag
     7321 taactggctg aacacaccgt tgatgatctg cgctacctgc tgtacctgag gcttttcgtc
     7381 gtactgttcc ggcatgttgt tggcgatccg acgcatctgc tcacggtcaa agttaaccat
     7441 ctgtgcggcg atgtttttca taaatccacc ccgtaaatcc agtctgtgtt tgtcaggtcg
     7501 agttttggtt tactggctgt cacgactgcc tgttgcttgt tacggttgat ttcgagctgg
     7561 gtccacttgt cgcggagttt ggccgggctc agcacattac cggaccagaa gttgtcctgg
     7621 caggcccagc ggaacagcac acacatatcg cggtggttac gtccgtcacg ttcacgcatc
     7681 aggcggatat cgttagccca ccctgcaaaa ttcggttttc tggctgatgg cgcgatagtc
     7741 ttcaccatgt caaacatcca ctctgcggcg gtcaggtctt ctgctgtccc ccatttgctg
     7801 ccgctctgaa ttgcagcatc aggtttcacc acagaaaggt cgttttctgg ctggtcagag
     7861 gattcgccag aattctctga cgaataatct tttctatttt cttttgtatt agtgtctttt
     7921 gtgttcccct gttttgaggg atagcaatcc cccaatttga gggatgtttt atccctcgtt
     7981 ttaggggatt ttccctcgtt ttgagggat
//
LOCUS       DAZFXT010000184         7808 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.184, whole genome shotgun sequence.
ACCESSION   DAZFXT010000184 DAZFXT010000000
VERSION     DAZFXT010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7808
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.184"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..1304)
                     /locus_tag="ACHKW0_004544"
     CDS             complement(33..1304)
                     /locus_tag="ACHKW0_004544"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0019985 - translesion synthesis [Evidence
                     IEA];
                     GO_process: GO:0042276 - error-prone translesion synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HFO7477230.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVESYSIDE
                     MFIDLREVNRCISPEVFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPEKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLPLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            complement(1304..1741)
                     /gene="umuD"
                     /locus_tag="ACHKW0_004545"
     CDS             complement(1304..1741)
                     /gene="umuD"
                     /locus_tag="ACHKW0_004545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HFO7477231.1"
                     /translation="MSTVYHRPADPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            complement(1738..1986)
                     /locus_tag="ACHKW0_004546"
     CDS             complement(1738..1986)
                     /locus_tag="ACHKW0_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HFO7477232.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERIMSLLEEIWQDDSWLPAA"
     gene            2404..3306
                     /locus_tag="ACHKW0_004547"
     CDS             2404..3306
                     /locus_tag="ACHKW0_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HFO7477233.1"
                     /translation="MYFSAEPAQIAEIKRLASGAVTPFYRRATNEGIQLFLAGSAGLL
                     QTTEDVQFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKNMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGDNFIQVDF
                     DTPWCQPESDVVAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDNVAHYGG"
     gene            3303..3614
                     /locus_tag="ACHKW0_004548"
     CDS             3303..3614
                     /locus_tag="ACHKW0_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477234.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLASVTVYASLSPFSDERPSSRFRGITLPSNRRRLRYSTVGLTRYRT
                     R"
     gene            3690..4373
                     /locus_tag="ACHKW0_004549"
     CDS             3690..4373
                     /locus_tag="ACHKW0_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HFO7477235.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWHRIDRFMAAWKRAGFSVVGHLVFTKNYT
                     SKSAYVAYRHECAYILAKGSPARPRNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            4374..4595
                     /locus_tag="ACHKW0_004550"
     CDS             4374..4595
                     /locus_tag="ACHKW0_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477236.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            4576..5043
                     /locus_tag="ACHKW0_004551"
     CDS             4576..5043
                     /locus_tag="ACHKW0_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7477237.1"
                     /translation="MRALKIEKETRMYGTCETLCRELAAKYPGDTPLMLVIWSPEEIQ
                     ALADGMDISLSDHEIRTVLAHLEDIPEDQRTESGISSGAAMEIIKYVSENRQVTVPAE
                     LLASLIQTAEQALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            5088..5858
                     /locus_tag="ACHKW0_004552"
     CDS             5088..5858
                     /locus_tag="ACHKW0_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477238.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNYPGWISIYVQ
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQALPVLPADGTQVSF
                     PYAGEWLTEEEIRAVFDAVRNAVRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            6274..6699
                     /locus_tag="ACHKW0_004553"
     CDS             6274..6699
                     /locus_tag="ACHKW0_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7477239.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGLDFLYAEASVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHLVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            6746..7165
                     /locus_tag="ACHKW0_004554"
     CDS             6746..7165
                     /locus_tag="ACHKW0_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HFO7477240.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFEHGADVSIVHDVVRELMEEKRVNRLVTVPAVLLEKVMVMAGSEI
                     YRLHAVGSENGGDGDAFVREEREIMRVMRQALDGENG"
     gene            7165..7356
                     /locus_tag="ACHKW0_004555"
     CDS             7165..7356
                     /locus_tag="ACHKW0_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477241.1"
                     /translation="MNISKETREILHNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRHR"
     gene            7319..7672
                     /locus_tag="ACHKW0_004556"
     CDS             7319..7672
                     /locus_tag="ACHKW0_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477242.1"
                     /translation="MVTTSFRAAGTGDTGQAVEAACQPARKPLAGWRLPEGMRATLAR
                     RPLPFLLVTGVVSGSTCYRPVVKEPSYTCAPSAPGCLAASGRQARLRQAAACAPASLR
                     RTRWRGFGLPLRSDA"
     gene            complement(7672..7740)
                     /locus_tag="ACHKW0_004557"
                     /pseudo
     CDS             complement(7672..7740)
                     /locus_tag="ACHKW0_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052655.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pilus protein"
BASE COUNT         1616 a         2161 c         2267 g         1764 t
ORIGIN      
        1 caagataaca atattatggg tggattacat attcataact gggctactgg caaatctgtc
       61 catctggtgg tatacgcagg agaaagcatt tcccgtttca tttgccactc aggggcggtt
      121 cctcgccccg caaaccacac tttccctttt ccagactggt tcagttcatc gagtgttttc
      181 attaactttt cgctgttttt acggggctgg atctcatcaa ataatcccgg ctgcgctata
      241 cccgatggtg tgaaatcagc cagcatgact cctgccttca tatagcggta cccttcacgc
      301 cagacatggt ttaacgctct gcatgctgcg gcaataatgt cccggctgtc ctgtgtgggc
      361 aatggaagct tttccacagc ggcattgctg taacagggtt cttttactgc aaagggggat
      421 gtccgtacaa atgtcgtcac ctgccggcaa tactgacgct ccccacgtag tttctctgca
      481 gcccgctctg catactgaac aacagcctgg tgcatggcat ctttgtctgt gattcgttca
      541 ccaaaactgc gactacagac aatctgctgt ttttccggtg gtgcttcttc cagggatatg
      601 caggactcgc cgttgagttc gcgtaccgta cgctcaagaa tgacgctgaa gtttttccgg
      661 atgaatgccg tgttagcctg cgccagctgc agtgctgtgt taatacccag cgcattcagc
      721 ttttccgtca gtctgcgtcc tactccccag acctcaccaa ctggctgcag ccccagtagc
      781 ttcaagatcc gattacggtt ttctgccgtc agcgcgacca caccggaaaa ctgtggccat
      841 tgctttgttg cccactgtgc acttttagcc agcgtttttg taggtgcaat gcccaccccc
      901 atggtgagtc ctgtccagct ctttacctgt tccctgagct gatgaccaaa aacctccgga
      961 gagatgcaac ggttcacctc ccgcaaatca atgaacattt catcaattga gtaggactca
     1021 actgcgggag aaagcgactc cagaacagcc ataacccgtt ggctcatgct gtggtacagc
     1081 gcataattgc tggaaaatac atgtattttc ttctccaggc gcatctgtct cacctgaaac
     1141 cagggctgcc ccattctgat gccaagggct tttgcctccg ggctgcgcgc gatcacacag
     1201 ccatcgttat tgctgagtac gatgaccggt tcgttgcgaa ggtccgggcg gaaaactttt
     1261 tcacatgagg cgtagaaact gttgatatca gccagtgcaa acatcagcgt aactcccgcg
     1321 tcctgtgtat cacgtgagtg acaacaccaa aaatacagat gttttccgga tacagtgtgc
     1381 ggaattccgg gctgtctgaa accggctcca gtgccgggcg tgggcgcaac agcagtcgtt
     1441 tgacggtgaa ctcaccgtcg atctcagcga taacgatgtc cccgtgttgt ggtttttcgg
     1501 ccctgtccac taccagcaga tcaccattct gcacgccagc ctggttcatc gattcaccgc
     1561 tggcgcgcag aaagaaggtg gctgcaggtc tgctgatgca atagctgttc agatccagtt
     1621 cctgctcagc ataatcagtg gcaggcgaag gaaaaccggc ctggcaacga tcggcaaaca
     1681 acggtcgcac gtaactgtca tcgcctgaag ggtcagccgg acggtgataa acggtactca
     1741 tgctgcaggc agccagctgt cgtcctgcca gatttcttcc agcagagaca tgatacgttc
     1801 tttatccttg tcgttgcggg ctcctgtgac agatacagac ggggcgctgc cctggtctat
     1861 acgcagccag atttccgggt agtgtggttt cagacgttgt tcgatttcat tctgtagcgc
     1921 ctgaagtgtg cctgatttga ggttttttgt gctctggcgg tcgaaaagaa tttcaatgcg
     1981 gatcatgttc tcatctccct tttctgttgc tgtatgcata tacagtattc taataactga
     2041 atccacacac agtcaaatgt cattattgtg actaaaaaac gctcaattct ctgagggggt
     2101 ggctgtatgc ctgctgtgga ctgctgtgtc cagcttatcc acagcatttt gcgcacggtt
     2161 ctgtgaataa aatacctggt tacccaggcc gtgccggcac gttaaccggg ctgcatccga
     2221 tgcaagtgtg tcgctgtcgc cggcctcctc acccggtcac gtttcgtcgt tcctcctcca
     2281 cgcgctgcgg cttcggggcc gcacctgcat tcgtatgcgg tcgcccggtt acaggtgcgg
     2341 cacggcctga tggaggccgc atgtgagagg agaattccca tgccaaactg gtgcagtaac
     2401 cgtatgtatt tttctgccga accggcacag atcgctgaga ttaaacgact ggccagcggc
     2461 gcagtcacac cgttttatcg ccgcgccaca aatgaaggta ttcagctgtt tctggccgga
     2521 agtgccggac ttctgcagac cactgaagat gtgcagtttg aaccgtgccc cggactgacg
     2581 gctgccggac gtggtgttgt atcgccggag aatatcgcgt tcacacgctg gctgacacac
     2641 ctgcagaacg gtgtgctact ggatgaacaa aactgcctga tgctgcatga actctggctg
     2701 cagagcggta ctggacagcg tcgctgggaa ggattaccgg acgatgtcag ggataccatc
     2761 accgcacttt tcaccgcaaa aagaggtgac tggtgtggct tctggagtaa cgaggatgta
     2821 tcggtgtggt ggaaccgtct gtgtgacaac gtactgccgg aaaaaaacat gccgtttgac
     2881 ctgctgacgg ttctgccgac ccgcctggat gttgaagtga atggctttaa cggcggtgtt
     2941 ctgaacggtg ttccttctgc atatcactgg tatacagaac ggtatggcgt gaagtggcct
     3001 tgtgggtatg acctgaatat cagcagtcag ggagacaact tcattcaggt ggatttcgac
     3061 acgccgtggt gtcagccgga aagcgacgtt gttgcagaat taagccgccg tttcagctgc
     3121 acgctggagc actggtatgc cgaacagggc tgtgatttct gtggctggca gttgtatgag
     3181 cgcggagagc tcgttgatgt gctgtggggg gaactggaat ggtcttcccc gacagatgac
     3241 gatgagctgc cggaagtcac cggacctgcg tggatagtcg acaatgtggc gcattatggc
     3301 ggatgaagta tgacggagac gggcgggcaa ccgcccgttt cttttccggt aaaggatgtc
     3361 gccgtgctcc tctttttact gcgccggctg acgcggcgcg gcataaacgc tgcctgtggt
     3421 cagtgtcccg cgtccggcgg gcacgggacg ggcgctttta ccgctcccgg ctggcgtcgg
     3481 tgacagtgta cgccagcctg tcgccctttt ctgatgaacg cccttcaagc cgctttcgcg
     3541 gcataacctt gccgtcaaac agacgacggc tgcggtattc cacggtcggc ctgacccgtt
     3601 accggacgcg gtgaacagcc cacaggcagc ggggaacggg caccacaggg gtgccctccc
     3661 ggtgcccact gtaagagaag gagttttgta tgtcccgttt tgtcctcggt aactgcatcg
     3721 atgttatggc ccgtatccct gataacgcca ttgatttcat cctcaccgac ccgccgtacc
     3781 tcgtcggttt ccgtgaccgt tccgggcgca ccatcgctgg cgataaaacc gatgaatggc
     3841 tgcaaccggc ctgtaatgaa atgtaccgcg tactcaaaaa agacgcgctg atggtcagct
     3901 tctacggctg gcaccgtatt gaccgcttta tggcggcctg gaaacgcgcc ggattcagcg
     3961 tggttggcca cctggtcttc accaaaaact acacatccaa atctgcgtat gtcgcgtacc
     4021 gccacgaatg cgcttacatc ctggcaaagg gcagtccggc ccggccccgt aacccgctgc
     4081 cggacgtgct gggctggaaa tattcgggca atcgccatca cccgacggaa aagcccgtta
     4141 ccagcctgca accgctgatt gagagcttca cacacccgaa cgcaattgtg ctggacccgt
     4201 ttgcaggcag cggctcaacc tgcgtcgccg ccctccagtc cggacgccgg tatatcggta
     4261 tcgagctgct tgagcagtat caccgtgccg ggcagcaacg ccttgccgcc gtgcaacggg
     4321 ccatgcagca gggggccgcg aatgatgact ggtttatgcc ggaggctgcg taaatgaact
     4381 atgccggaca cgaaaaactg cgcgccgaag tggcggaggt ggccaatgcc atgtgcgacc
     4441 tgcgtacaac catgaatgag atggagcagc ggtacagctt taatgccgac accctgccgg
     4501 aacgtctggt gcgtcagacg ctgtttcgcg caaaccgcct cctgatggag gcatataccg
     4561 aaattctgga actggatgcg tgctttaaag attgagaagg agacgagaat gtacggaaca
     4621 tgcgaaacgc tatgccggga gctggcagca aagtatccgg gagacacgcc gctgatgctg
     4681 gttatctggt ccccggaaga aattcaggcc ctcgctgacg gaatggatat ttccctgtcc
     4741 gatcatgaaa tcaggactgt cctggcccac ctggaggaca tcccggaaga ccagcggact
     4801 gaatccggta tttcttccgg tgcggcgatg gagattatca aatacgtgag cgaaaaccgc
     4861 caggtgaccg tccctgctga actgctggcg tccctgattc agaccgctga acaggcactg
     4921 tggaaacgtg agtgggccgc ccgtgataac ggcctcgccg ttccggaatg cgtcacccgc
     4981 cgacaggcgg tggttaatca ggcccgcgcc ctgctgaaaa acaacacacg cgaaaacgac
     5041 tgatgttatc gccgcctgcg ggcggcgatt cagggagacc atgaatcatg aacgaaacgc
     5101 tgaatgcact gatttgtcgc cacgcccgta acctgctgct ggcgcagggc tggccggaag
     5161 agacggatgt tgaccagcga aatccgaact atccgggctg gatcagcatt tacgtgcagc
     5221 tggatgcgcc ccggctggcg acgttactcg ttaaccgcca cgatggcgtg ctgccgccgc
     5281 acctggcctc cgccattcag aaactgaccg gaaccggagc ggaacttgta ctgtccggca
     5341 gtcagtggca ggcgctgccg gtacttccgg cagacggaac gcaggtgtct ttcccgtatg
     5401 ccggtgaatg gctgacggag gaagaaatca gggcagtttt tgatgcggtg cgcaatgcag
     5461 tacgaagcat ctgttaccag gtggcagacg atgcgcggcg tatccgggcg gcgctgacta
     5521 ccaccggtca gacgttgctg acccgccaga cgcgccgctt tcgtctggtt gtgaaggaaa
     5581 gcgatcaccc ctgctggctt gatgaagatg acgaaaatct gcccgtggtg ctcgatgcca
     5641 tcctgaaccg gggagcacgt ttttcggcgg tggaaatgta tctggtcagc gattgtattg
     5701 agcatatcct gtccagtggg ctggcctgcg atgtgctgcg tataccggat gaaccgcccc
     5761 gccggtggtt tgaccgtggt gttctgcggg aggtggtccg ggaagcccgg gccgaaattc
     5821 gcagcatggc ggatgccctg gcaaaaatcc ggaaatgatg acgctggcgg ggaaaaatct
     5881 ccgccttttc cagccggtcc ccggaaagag caaaaggatg tcgccgcagg ctccgcttta
     5941 cccggccatg cggggcatgg ccttgtgggt tttcagttat gtggcctcag cgtcgtgtgc
     6001 gggctgtgcc gtgcctccat cgtagccggg cttctgcggg cgggttgcaa ggtaaggtga
     6061 accgctgcgg tcacccggtc cctccttccc gtctgccgtg attttccggt cgttcacccg
     6121 ctcagatttc gcggtctcac cctccaactc ccgtcagccg tgcgcgtcag gctgcggctt
     6181 cccttgcgcc cttgcaaccc tgcaggcccg gcaaagatgg aggcacggca ccgcccggtg
     6241 tcgcggaata tgtgaacaac ggaggaaaac atcatgcaat atgcgaaacc tgtcactctg
     6301 aacgttgaag agtgcgaccg tctctccttt ctgccttacc tgtttggcct ggattttctg
     6361 tatgccgaag cgtctgtata cgcgctggcg caaaaaatga tgccggaata tcagggaggc
     6421 ttctggcatt tcatccgcct gccggacggt ggcggttaca tgatgccgga tggcgaccgt
     6481 tttcatctgg tcaacggtga aaactggttt gaccgcactg tgagtgctga tgccgcaggt
     6541 atcattctga cttcccttgt gattaaccga cagttgtggc tgtaccacga cagcggggat
     6601 gcgggactga cacaccttta ccggatgcgc gatgcgcagt tgtggagcca catcgaattt
     6661 caccccgaat gtaacgcgat ttacgcagca ctggactgat taacggacgg ggcggcgatg
     6721 ccgcccctgt aaaggagacg agaatatgta ctgtactgtt aaagaaatta tccgcgaagt
     6781 gctggataca gacgtgccgg acagtgaatg cgtttttgcc gtggtgctga cccgtgggga
     6841 tgtgcgtcac atagcccagg actggagtct gacagacgat gagctggaaa ccgtcatgca
     6901 gcggctggac gatgcctttg agcatggtgc tgatgtcagc attgttcacg acgttgttcg
     6961 tgaactgatg gaggaaaagc gcgtaaaccg tcttgtaaca gtccctgcgg tgctgctgga
     7021 aaaagtgatg gtgatggctg gtagtgaaat ttatcgcctc catgctgtag ggagcgagaa
     7081 cgggggcgac ggtgacgcat ttgtcaggga agaacgtgag attatgcggg tgatgcgtca
     7141 ggcgctggac ggggagaatg ggtaatgaat atcagcaagg aaacccgcga aattctgcat
     7201 aattacaggg ccgtgattaa tgcgcggcgt cgtgaaatgg ggcagaaacc gctcaccacc
     7261 gcgcagattg ttgatgaaat ctgcgatttt gtggcgaatc agcaggcggt tttcctcggt
     7321 ggtcactaca tccttcaggg cagcaggcac aggtgataca gggcaggcgg tggaggccgc
     7381 ctgtcagccc gcgcgaaaac ccctggcagg ctggcgcctg ccggaaggga tgcgggccac
     7441 gctggcgcgt cgcccgttac ccttcctgtt ggtgacaggc gttgtttcag gaagtacctg
     7501 ttacagacct gtcgttaaag aaccttctta cacatgcgcc ccttcggctc ccggctgcct
     7561 ggcggcatcc gggcgtcagg cccgactccg acaggcagct gcttgcgcac ccgccagtct
     7621 ccgccgaacc cgctggcgcg gtttcgggct gccgctacgg tccgatgcgt gaaaacccgg
     7681 gcttgcagcg accgttccgg tcgcgccgcc cctgtcgcgc cgtgacctgc gctccctgca
     7741 cacctgacgg tgtgtgagga aactgcgcct gatggcgcag cagtcttccc tcctgcagcc
     7801 acccctgc
//
LOCUS       DAZFXT010000185         7743 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.185, whole genome shotgun sequence.
ACCESSION   DAZFXT010000185 DAZFXT010000000
VERSION     DAZFXT010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.185"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHKW0_004558"
     CDS             complement(54..1217)
                     /gene="fadA"
                     /locus_tag="ACHKW0_004558"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HFO7477243.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHKW0_004559"
     CDS             complement(1227..3416)
                     /gene="fadB"
                     /locus_tag="ACHKW0_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0036125 - fatty acid beta-oxidation
                     multienzyme complex [Evidence IEA];
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004165 - delta(3)-delta(2)-enoyl-CoA
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HFO7477244.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAANGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHKW0_004560"
     CDS             3606..4937
                     /gene="pepQ"
                     /locus_tag="ACHKW0_004560"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HFO7477245.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4937..5551
                     /locus_tag="ACHKW0_004561"
     CDS             4937..5551
                     /locus_tag="ACHKW0_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HFO7477246.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            5590..7041
                     /gene="trkH"
                     /locus_tag="ACHKW0_004562"
     CDS             5590..7041
                     /gene="trkH"
                     /locus_tag="ACHKW0_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HFO7477247.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            7053..7598
                     /gene="hemG"
                     /locus_tag="ACHKW0_004563"
     CDS             7053..7598
                     /gene="hemG"
                     /locus_tag="ACHKW0_004563"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HFO7477248.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDHCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         1715 a         2096 c         2108 g         1824 t
ORIGIN      
        1 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
       61 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
      121 tggacgtctt tgcgctccat cagatttagc agcgtggtgc tgatacgcgc accggaacag
      181 cccagcggat gacccagcgc gatcgcgcca ccgttgaggt tgatcttctc gtcaatctgc
      241 tccattagcc ccagatcttt aatacatggc aggatctgcg cggcaaaggc ttcattcatt
      301 tcaaacacgc cgatatcact gacagaaagc cccgcttttt tcagcgccag cttcgaggcc
      361 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcacg
      421 cgggcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
      481 gctgcgccat cggaaagcgc agaagatgta cccgccgtta ccgtaccgct tactggatca
      541 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
      601 taattaaact gcttcaggac gccgtcggca tcgtgaccac cggtcgggat aatttcattt
      661 ttaaatgcac ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
      721 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
      781 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtggctc
      841 attggcacat ggcccatatg ctccacgccg ccgacaagac atgcctgcgc atcgccagtc
      901 atgatcattc gtgctgcgtc atgcaatgcc tgcatggatg aaccacacaa gcgattgacg
      961 gtcaccgccg ggacagagtg tggtacttct gccagcagcg ccgcgttacg ggcgatatta
     1021 aaaccctgct ccagcgtctg ctgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
     1081 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttctgca
     1141 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
     1201 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
     1261 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
     1321 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
     1381 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
     1441 accagcgcca tatccgcttc cgccggagtg gcgataatgc cttcctccag acagcgcacc
     1501 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
     1561 ttcggctggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
     1621 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
     1681 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
     1741 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacgac atccagcaga
     1801 tatgccggac ccataggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
     1861 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
     1921 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
     1981 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
     2041 ggcatccggt gtaccgggtt aaaaaagtgc attccgcaga agttttccgg gcgttccagc
     2101 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatccggg
     2161 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcgacaacc
     2221 gcttctacca caacatccac gcggtcaaac ccggcgtagt cgagcgttgg gtggattgtg
     2281 gagatcacgc cagccagttt cagaccatcg atcttgccgc gctcaagctg cttgttcagc
     2341 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
     2401 accggcacgc ctttccacgc agactggtaa gcgatgccgc cgcccataat gcctgcaccc
     2461 agcaccgcgg cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
     2521 acatattgat cgttaaggaa aatgccgacc agtgcgcggg cttcgttggt atgcgccagc
     2581 gggacaaaac ttttgttttc caggtttaag gcttcttcac gaccaaaacg ggccgcagct
     2641 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgctgt ttgtgcgacc
     2701 atccctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagtggttcc
     2761 agcttcggct gacgttttgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaacc
     2821 gcctttgcgc cttcaaccag tttttctgct ttgactacgc catccaccag accgattttc
     2881 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagcgc actgtcagcg
     2941 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
     3001 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
     3061 tcgcagccac cgcccagcgc atagccgttg gcggcagcaa tggtcggcac cggcagatct
     3121 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
     3181 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
     3241 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
     3301 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
     3361 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
     3421 gtctcctgaa tccgcaaggt gatctggtcg taccagatga gtcgaagtgt gtattttgtg
     3481 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
     3541 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
     3601 aaaaaatgga atcactggcc tcactctata aaaatcatat agctacctta caggaacgga
     3661 ctcgcgatgc gctggcgcgc ttcaagctgg atgcgttact tattcactcc ggcgaactgt
     3721 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
     3781 gggtgccggt aactcaggtg ccaaactgct ggctgctggt ggatggcgtg aacaagccga
     3841 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctctt
     3901 tctggactga agacgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
     3961 tgcctgctgc gcgcgggaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
     4021 tgggtattga ggccagcaat atcaacccga aaggggtgat cgactacctg cattactacc
     4081 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
     4141 acggtcatcg cgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaaca
     4201 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
     4261 cgctcaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
     4321 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
     4381 cccgtacctg gtcggcaaaa agtgacaacg actacgcaca gctggtgaaa gacgtaaatg
     4441 atgaacaact ggcgctgatc gctaccatga aagcaggcgt cagctatgtg gattaccaca
     4501 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
     4561 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
     4621 atccgctggg cctgcaggtg catgacgtag ccggttttat gcaggatgat agcggtacgc
     4681 acctcgcggc accggcaaaa tatccgtacc tgcgctgcac ccgtattctc cagccgggca
     4741 tggtgttaac catcgaaccg ggtatctact tcattgaatc gctgctggca ccgtggcgtg
     4801 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
     4861 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
     4921 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
     4981 agagatcaaa aaaagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
     5041 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
     5101 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
     5161 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
     5221 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
     5281 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
     5341 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
     5401 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
     5461 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
     5521 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
     5581 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
     5641 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
     5701 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
     5761 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
     5821 accgtgctgg gtagcgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
     5881 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
     5941 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
     6001 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
     6061 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
     6121 attgcggaaa cggcgaaaac cctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
     6181 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
     6241 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagcccgact
     6301 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
     6361 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
     6421 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
     6481 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
     6541 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
     6601 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
     6661 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
     6721 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
     6781 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
     6841 acgggcgtgg atgacttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
     6901 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
     6961 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattgctggt gctctttacc
     7021 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
     7081 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
     7141 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aattatgacc
     7201 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
     7261 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
     7321 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
     7381 tgaactcgca atggcgtccc gatcactgcg cggtcattgc cggggcgctg cgttacccac
     7441 gttatcgctg gtacgatcgt tttatgatca agctaattat gaagatgtca ggcggtgaaa
     7501 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
     7561 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
     7621 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
     7681 accactgaca cggaacaacg gcaaacacgc cgccgggtca gcggggttct cctgagaatt
     7741 ccg
//
LOCUS       DAZFXT010000186         7719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.186, whole genome shotgun sequence.
ACCESSION   DAZFXT010000186 DAZFXT010000000
VERSION     DAZFXT010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.186"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1326
                     /gene="xanP"
                     /locus_tag="ACHKW0_004564"
     CDS             <1..1326
                     /gene="xanP"
                     /locus_tag="ACHKW0_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HFO7477249.1"
                     /translation="IYRLEDRPPLPQTLFAACQHLLAMFVAVITPALLICQALGLPAQ
                     DTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQGTSFNFVAPLIMGGTALKTGGAD
                     VPTMMAALFGTLMLASCTEMVISRVLHLARRIITPLVSGVVVMIIGLSLIQVGLTSIG
                     GGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNPYLRVASLVIAMAAGYALAWFMG
                     MLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLMLVFMITSLETIGDITATSDVSEQ
                     PVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCFGQNNGVIQLTGVASRYVGFVVA
                     LMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIAASGVRIVSREPLNRRAILIIAL
                     SLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGITAIVLNLIFPPEKQ"
     gene            1447..3156
                     /gene="yicH"
                     /locus_tag="ACHKW0_004565"
     CDS             1447..3156
                     /gene="yicH"
                     /locus_tag="ACHKW0_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HFO7477250.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(3209..5527)
                     /gene="yicI"
                     /locus_tag="ACHKW0_004566"
     CDS             complement(3209..5527)
                     /gene="yicI"
                     /locus_tag="ACHKW0_004566"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HFO7477251.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRQDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(5537..6919)
                     /gene="yicJ"
                     /locus_tag="ACHKW0_004567"
     CDS             complement(5537..6919)
                     /gene="yicJ"
                     /locus_tag="ACHKW0_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HFO7477252.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            7212..7306
                     /locus_tag="ACHKW0_004568"
     tRNA            7212..7306
                     /locus_tag="ACHKW0_004568"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7246..7248,aa:Sec,seq:tca)
     gene            7385..7546
                     /gene="rhuM"
                     /locus_tag="ACHKW0_004569"
                     /pseudo
     CDS             7385..7546
                     /gene="rhuM"
                     /locus_tag="ACHKW0_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774140.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RhuM family protein"
BASE COUNT         1854 a         2053 c         1940 g         1872 t
ORIGIN      
        1 atttaccgtc ttgaagatcg tccgccgctt cctcaaaccc tgtttgccgc ctgtcagcat
       61 ctgctggcga tgttcgttgc ggtgatcacg ccagcgctat taatctgcca ggcgctgggt
      121 ttaccggcac aagacacgca acacattatt agtatgtcgc tgtttgcctc cggtgtggca
      181 tcgattattc aaattaaggc ctggggtccg gttggctccg ggctgttgtc tattcagggc
      241 accagcttca actttgttgc cccgctgatt atgggcggta ccgcgctgaa aaccggtggt
      301 gctgatgttc ctaccatgat ggcggctttg ttcggcacgt tgatgctggc aagttgcacc
      361 gagatggtga tctcccgcgt tctgcatctg gcgcgccgca ttattacgcc gctggtttct
      421 ggcgttgtgg tgatgattat cggcctgtcg ctaattcagg ttgggttaac gtccattggc
      481 ggcggttacg cagccatgag cgataacacc ttcggcgcac cgaaaaatct gctgctggca
      541 ggcgtggtct tagccttaat tatcctgctt aaccgtcaac gtaaccctta cttacgcgtg
      601 gcctcactgg tgattgcgat ggcggccgga tatgcgctgg cgtggtttat gggcatgttg
      661 ccagaaagca acgaaccgat gacgcaagaa ctgattatgg tgccaacgcc gctctattac
      721 ggtcttggca ttgaatggag tctgctgctg ccgctgatgc tggtctttat gatcacttcg
      781 ctggaaacca ttggcgatat cacggcgacc tctgacgttt ccgaacagcc agtgtccggt
      841 ccgctgtaca tgaaacgcct gaaaggcggc gtgctggcaa acggcctgaa ctcgtttgtt
      901 tcggcggtgt ttaacacctt cccgaactcc tgcttcgggc aaaacaacgg agtgatccag
      961 ttgactggtg ttgccagccg ctatgtcggt tttgtcgtcg cgctgatgtt gatcgtgctg
     1021 ggtctgttcc cggcagtgag cggttttgta caacacattc cagaaccggt tctgggcggc
     1081 gcaacgcttg taatgtttgg caccatcgcc gcctccggtg tgcgtatcgt ttctcgtgag
     1141 ccgctgaacc gtcgggcgat tctgattatc gcgctgtcgc tggcggttgg tctgggcgtg
     1201 tctcagcagc cgctgatttt gcagtttgcc cctgaatggc tgaaaaacct gctctcctcc
     1261 gggatcgccg cgggcggtat tactgccatc gtgctgaatc tgattttccc accagaaaaa
     1321 cagtaattca ctggcggcgg taagataatt accgccgcag tctcacctct ttcaccattc
     1381 ccgccttgag gattgcgcgt aaatcgtgca taactccctt atgtgcattt cacgggatgg
     1441 aagaccatga aatttattgg gaagctgctt ctctacattc tcatcgctct gttagtggtg
     1501 atcgctggcc tctattttct tctgcaaacc cgctggggag cagaacatat cagcgcatgg
     1561 gtttccgaga atagcgacta tcatctggcc ttcggggcga tggatcaccg tttttccgcg
     1621 ccatctcata tcgtgctgga gaacgtcacg tttggtcgtg atggtcagcc cgcgaccctg
     1681 gtggcaaaaa gtgtcgacat tgcgctaagc agtcggcaac tgaccgaacc acgccatgtc
     1741 gataccatcc tgctggaaaa cgggacgctg aatctcaccg accagaccgc gccgctaccg
     1801 ttcaaagccg atcgtctgca actgcgtgat atggcgttta atagcccgaa tagcgaatgg
     1861 aaactgagcg cgcagcgggt aaatggcggc gtggttccgt ggtcaccaga agccggtaaa
     1921 gtgctgggta cgaaggcgca gattcagttt agtgccggat cgctttcgct caatgatgtt
     1981 cctgccacca atgtactgat tgaaggcagt attgataacg atcgcgttac gctgactaac
     2041 ctgggtgccg acatcgcccg cgggacatta accggaaacg cgcagcgtaa cgccgacggc
     2101 agctggcaag tggaaaatct gcgcatggcg gatatccgtc tacaaagcga aaaatcgcta
     2161 accgacttct ttgcgccatt acgctctgtc ccgtcgttgc agattggtcg cctggaagtg
     2221 atcgatgctc gtttgcaagg tccggactgg gcggtgaccg acctcgatct cagcttgcgc
     2281 aacatgacct tcagtaaaga tgactggcag acacaagaag gcaaactgtc gatgaacgcc
     2341 agcgagttca tttatggttc gctgcattta tttgacccga ttataaacgc ggaattttcc
     2401 ccgcagggcg tagcgctgcg ccagttcacc agccgctggg aagggggtat ggtcagaacg
     2461 tcagggaact ggctgcgtga cgggaaaacg ttgatccttg atgatgcggc aattgccggg
     2521 ctggaatata ccttgccgaa aaactggcaa cagttgtgga tggaaacgac acccggttgg
     2581 ttaaacagcc tgcaactgaa gagatttagc gccagccgca atctgatcat tgatatcgac
     2641 cctgacttcc cgtggcagct caccgcgctc gatggttacg gtgccaacct gacgctggtt
     2701 accgatcata aatggggcgt ctggagtggc tcggcgaatc tgaatgccgc cgccgcgaca
     2761 ttcaatcgtg ttgatgttcg tcgcccgtcg ctggcgctga ccgccaacag cagcacggtg
     2821 aatatcagcg aactgagtgc atttactgaa aaaggcattc tggaagccac cgccagtgtt
     2881 tcacaaacgc cacaacgtca gacacatatc agcctgaatg gacgcggtgt gccggtgaat
     2941 attttgcaac agtggggatg gcctaaatta ccgttgactg gcgacggcaa tattcagctt
     3001 accgccagtg gcgatattca ggccaatgtc ccgttgaaac ctacggttag cgggcaattg
     3061 catgccgtga acgccgcaaa gcagcaagtg actcaaacca tgaatgctgg catcgtttcc
     3121 agcggtgaag ttacatcgac ggagccggtg cggtaaacat caatctcgcc gggtacgact
     3181 cagacgtacc cggcattcca tcaatagatt acaacgtaat tgtcagcgca ttcccttgag
     3241 gcttcaccac cagcccctgc tcactttcag cctgcgaacc gtcttgcaga ccatttactt
     3301 tcacaacatt gcgcaggcac agcgtccagt tcttcgcctc gcccgcacca gtcacagtaa
     3361 tcgtgttgcc agtacgtgct gcttttaaag taaagatcac cgatccgtca gtagcgggca
     3421 cttcacagac ggcttcatgc ccgtcttgca gattaaagag gtggaatgcc gtgccttcgt
     3481 gccacacgta atcgggacgt tgatcgttgt tgcccagcgc cagtagagtg ttatcacgca
     3541 cataaacggg cagactcagg aagccgtgct gctgtttatg ccagcgacta ccgtcgagtt
     3601 catcgttgtg ccacaggtgt gtccagcgac cttccggcag gtagaactgc acatcgcccg
     3661 cttcagtgaa caccggcgca accatcacgt tgtcgcctaa catgtattga cggtcaaggt
     3721 aatcacaagc cggatcgtcc gggaactcca tcatcatggc ccgcatcatc ggcgtacccc
     3781 gcgcgttcgc acgcgcagct tcacgataca gatacggcat catgcggcat ttcagttgcg
     3841 tgaagaagcg caccacatca caggactcat catcgtaggc ccacggcaca cgataagatt
     3901 tgctaccgtg taaacggcta tggctggaga gcaaaccaaa cgcgcaccag cgtttgtaaa
     3961 cgtgcgccgg agcggtattt tcaaagccgc cgatatcgtg gctccagaag ccaaaacctg
     4021 aaaggccaat agacaaacca ccgcgcaggc tttccgccat tgattcgtag ttagcgtaac
     4081 aatcgccacc ccagtgtacc gggaatttct gcgcaccgac ggaggccgag cgggcaaaca
     4141 agacagcttc ttcctcacca acggtgtcct tgagcacgtt ccacaccagt tcgttgtaga
     4201 tgtacgcata atggttatgc attttctgcg gatcggaacc gtcaaaccac tgaacatcag
     4261 ttgggatacg ttcgccaaag tcggtcttaa agcaatcaac gcccatcgcg accagacctt
     4321 tcagtttgtc ggcgtaccat ttgcaggcat ccggattggt aaagtcataa atcgccagac
     4381 ctggctgcca tttatcccac tgccatagcg aaccgtcctg gcgtttgagt aaatagcctt
     4441 tctcttgtaa ctctttaaag acgggggatt tttgaccgat ataggggtta atccagacgc
     4501 agattttcag tcctttcgct ttcaggcggc ggatcatccc ttccgggtca gggaaagtca
     4561 gcgggtccca ctcaaaatcg caccactgga aggctttcat ccagaaacag tcaaagtgga
     4621 aaacatgcag cggcagattg cgttccgcca taccatcgat aaagctgttt accgtcgctt
     4681 cgtcgtagtt ggtggtaaat gaagtggtta gccacaggcc gaaggaccac gcgggcggca
     4741 gcgccggacg accagtaaag cgggtataac gatcaagtac cgctttcggc gtcgggccgt
     4801 cgataacaaa gtattcgaga tattcactct caacgctgaa ctgcactttg gagactttct
     4861 ccgatcccac ttcaaaagag acacactggg gatgattgac cagtacccca taaccacggt
     4921 tagtcatgta gaacgggata tttttatacg cctgttcagt acttgtgccg ccgtcccggt
     4981 tccaggtctc taccgtctgg ccattgcgca ccagggcagt aaagcgctct cccagaccgt
     5041 aaactgtttc gccaacgcca agatcaagcc gctcaaacat ataattgcgt tgattattcg
     5101 tgtcctgcac gtagccatta tttttcacct gactaccggt aatacgttcg ccgttgcgca
     5161 gaaaatccag tgaccagaac tcacctttgc tgacacgcgc gcttaagttg ccacttttaa
     5221 actcagcata acgttctgtg ttttcgattg tgaccttcac gtcctgcaaa atattgagcg
     5281 gataatgagg accgttattc agcgccccct gaaaatgctc aatccgcaca ccgacaatac
     5341 cttcctgtgg ggagaaaaag cgcaacgtaa ataaaggcgt atcaagctgc caggtacgtt
     5401 cacgcacatc acggggggca gcatagacca ccatttcatt atcctgctgt tcaacctcga
     5461 acacctgaag cgggtgaatc aaattgaggc caggttgaat caaccagttt ccatcgctaa
     5521 ttttcattgc cgttccttag ttctgcactt cttgagaggt gaattgttgc tggcaacggc
     5581 gtttaccctg agccagctgt tccataacgg ttttcagatt gtgcgtcgtg agtgagtagt
     5641 agcgtttagc gataatcgcg ctcagcaaat aacagatcgc cggaacaatc gtgaatagcg
     5701 caataatgat gctaatcgtg gcgctgttct gcgctttttc tgccgcatca tatccgccat
     5761 aagccagcat ccagccgata agagcgccgc cgaaggccaa ccccagtttg agcacaaaca
     5821 gcgtgccagc aaaactgatc ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga
     5881 cggtatcgga catcattacc cactggatag gtgtcaccag ttgatgcaac acaccaatca
     5941 cgaagatgaa gacaaacata gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc
     6001 taatcactgc cagcagggcg ttcgtccacc agaagatagt gactttacat ttccagtcgg
     6061 tcagaggttt tgccagtgcg gaaccaatca ggttacccac gcaataagtg gtgagaaaag
     6121 cgacaaacac ttccggcgtg cccaaaatcc atgtgacgta atacatcatc gccccaccgc
     6181 gtacgcacac cgccaggata ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt
     6241 tttgccagat atcacgtaaa tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc
     6301 gttctttagt ggtgaagaaa caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg
     6361 cgataccgcc ctggaaaccg agtggtttat tatcaccgcc aattaaatta accagtggca
     6421 tcatcagaac agtagaaagc atgcctcccg cggtcgccag cacaaaacgc caggattgca
     6481 gcgagatacg ctgagtcggg tcattggtga ttacaccacc caatgcgcag taagggatat
     6541 tgacgacggt ataaagtaag gtaagtaggg tgtaagtaat tgctgcatag atcattttgc
     6601 cgttcatact gagatctggc gtgctatagg ccagtacaca gacgatcccg aatggcagtg
     6661 cgccaaacag tacccacgga cgaaatttac cccagcgaga gcgcgttcga tcggccaaca
     6721 accccatgca aggatcggaa atcgcatcca gtgcacgagc gaccaaaaac atggttccga
     6781 caaaaccggc aggaatgcca aaaatatcgg tataaaagaa catcatatat aacattacgt
     6841 tatcgaaaat aatgtggctg gcggcgtctc ccatgccata accaattttc tctttaacgg
     6901 acaacacttc actcttcatc tgcttttcct tgatgctata ggggtgctga gaccgataac
     6961 gcattttgta aaccatccct gtgagagcga atattccgat atctggttat taatttatga
     7021 ttcttgtttt atgtgatcgt ggtagcgtta atttcgctca tatatcattg taaaatatag
     7081 gttttatatg aactataatg ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac
     7141 tgatctgggg gatgtagaaa ctcaaggaag tagctataat gcgccccgcc tccatgtagc
     7201 aatcgaggcg cggaagatcg tcgtctccgg tgaggcggct ggacttcaaa tccagttggg
     7261 gccgccagcg gtcccgggca ggttcgactc ctgtgatctt ccgccaaaat gcctctcctg
     7321 acgttctcca accccttgta atactgtcaa tccagatatt ttctactgct caatattcaa
     7381 tcggcttcag gaatacctgg tctaaggttt tgtgatggac gatgagcggc tgaagaatcc
     7441 gcctgtcggt tcttctgtcc tcgaacgcat ccgcgatatt cacgccagta tgcgccgtgt
     7501 ttatttgcag gtgatttgcc cccctgctga ctctcagcca actgtatcgc cattaaggtg
     7561 aagatgttgt tttactcgcc ccagaacacc cttttttata cgactattct atttctttaa
     7621 ccgctggcga aaaaaaactg actataaact tcaccaataa tcagactgcc atcaagatta
     7681 atttgttcat ttaatatatc aaatcaactt gttttattt
//
LOCUS       DAZFXT010000187         7668 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.187, whole genome shotgun sequence.
ACCESSION   DAZFXT010000187 DAZFXT010000000
VERSION     DAZFXT010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.187"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(196..1569)
                     /gene="cysG"
                     /locus_tag="ACHKW0_004570"
     CDS             complement(196..1569)
                     /gene="cysG"
                     /locus_tag="ACHKW0_004570"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HFO7477253.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMGERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     MNWFSNH"
     gene            complement(1588..2394)
                     /gene="nirC"
                     /locus_tag="ACHKW0_004571"
     CDS             complement(1588..2394)
                     /gene="nirC"
                     /locus_tag="ACHKW0_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HFO7477254.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            complement(2521..2847)
                     /gene="nirD"
                     /locus_tag="ACHKW0_004572"
     CDS             complement(2521..2847)
                     /gene="nirD"
                     /locus_tag="ACHKW0_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HFO7477255.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            complement(2844..5387)
                     /gene="nirB"
                     /locus_tag="ACHKW0_004573"
     CDS             complement(2844..5387)
                     /gene="nirB"
                     /locus_tag="ACHKW0_004573"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HFO7477256.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTEDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            complement(5649..6830)
                     /gene="tsgA"
                     /locus_tag="ACHKW0_004574"
     CDS             complement(5649..6830)
                     /gene="tsgA"
                     /locus_tag="ACHKW0_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HFO7477257.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            7101..>7668
                     /gene="ppiA"
                     /locus_tag="ACHKW0_004575"
                     /gene_synonym="rotA"
     CDS             7101..>7668
                     /gene="ppiA"
                     /locus_tag="ACHKW0_004575"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HFO7477258.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVL"
BASE COUNT         1910 a         2029 c         1947 g         1782 t
ORIGIN      
        1 ataagcagac tgctcgttaa gaacgataca ggaatattta attggcgtca gtgagcgaat
       61 gacttaattt tgtaagtcgc tgtaacggtg aatataaccc attagcagat aatatttatt
      121 aaggggttat gttttatttg ctgaatatat aaaaagagtc attaagaagg ccagaattct
      181 ctggcccggg ttaaattaat ggttggagaa ccagttcatt ttatcgcgca ggccaacaac
      241 ccgaccaata ataattagcg atggactgtt catttgctga gcaagttcac cgagttgcgt
      301 gagcgtaccg tcaatcacgc gctgcgtgac tgccgttccg ttttcgacaa ttgccaccgg
      361 catttcgcca ggcataccgt gttcaatcag cttttgctga atagtcgcgg cctgattcaa
      421 ccccatatag aacaccagcg tctgtttttc tgccgccagg ttttcccagt ccagctcgcc
      481 accggttttt aagtgtccgg taattaagcg tacgctctgg gcataatcgc gatgcgtgag
      541 tggaataccc gaataggcag agcaaccaga agctgcggta atacccggaa ccaccgagaa
      601 cggaataccc gcgttgcaca gtgtttccag ctcttcgcca ccacggccaa aaataaacgg
      661 atcgccacct ttcagccgca ccacgcgttt gcctttttgc gcttcccgca gcaggatctg
      721 gttaatctct tcctgcggta cacagtggta tcccgcgcgt ttgccgacga aaacgcgatc
      781 agcatcgcgg cgtaccagat tcataatatc gtcagaaacc agacggtcgt agaccaccac
      841 atctgcctgc tgaatttgtt gcagtccttt cagcgtcagc agcccggcat cgcccggacc
      901 tgcaccaacc agcaccactt caccgcgatg gtcaagcggt tcgttgatta actgttcggt
      961 ggtttcagta atggctttct gatcgttgtt tgccagcgac tgcgccaggc gatcgttaac
     1021 gaacaatttc tcccagaaac ggcgacgctc acccatcgtg gcgaactgtt gtttcactcg
     1081 cccgcgtaat tgcccggcgt atttcgctac ctggcccaga tgcaacggca gcagtgattc
     1141 aagtttttcg cgcaacaggc gtgccagaac cggagaggtg ccgccagagg agaccgctac
     1201 catgagcggt gagcggtcaa taatcgacgg cataataaag ctggcggctt tcggcgcatc
     1261 gaccacgtta cagaagatgc gacgagcttc agcggcttcg ctgacgcgct ggttaagcgc
     1321 gtcatcatcc gtcgctgcaa tcgccagcca gcaggtgtcg agaaggcttt catcaaatgg
     1381 cccttcgacg agggttaaca tgcctgcatc tgcccatgcg gtgaactgtg gaataaacgc
     1441 taatgcattc accgttaagc gagcgcctgc gtctaacagc aaccttgctt tgcgttccgc
     1501 gacatcacca ccgccgacaa tcagacagtc gcgatcgcgt aattggcaaa atataggcaa
     1561 atgatccacg taaaaacccc ttagtaatta accggcagcc gtttcagttt gattaaattt
     1621 gtccgcaacc ggacgattcg ctttcggcgt agcataccaa taacccaatc ccatgaatac
     1681 ggcacctgat aaagtattac ccagcgtcac ccacagcagg ttatgaccaa tacccgccag
     1741 cgtgtaggct tcgctgtggt tgccgaacca ggagagcgcg aacagcgtca tgttagcgat
     1801 agagtgctcg tagccagacg cgataaatgc cagcagacac caccagatag cgataaattt
     1861 cgccgcccct tcagtgcgca gcgccatcca gattgccagg caaaccagcc agttacacaa
     1921 tgcacctttg aagaacagta ccattgccgg tgcagtggtt ttagccagcg cgacggagtg
     1981 aacgatgctg gtatctaccg gcagcaggct accgccgccc cagctataga gcatggcaac
     2041 gaagacggaa ccgaccaggt tacccagcca ggtttgcggc aggattgccc acatttgccc
     2101 gtggctgatg ctgcccgctt ttaccccaaa ggtgaggaac atggtgtgtc cggtgaacag
     2161 ttcagaaccg gcgataatca ccagcgttaa ggcgatacca aaggtcgcgc ccatcaccag
     2221 cggacgtacg gacgggtcga gcaaattacc gagcgtgaaa atcaagatga tcccaagacc
     2281 cacatacgcg cccgccatgg cggagctgac ccaaaagccg agcgggttat ttgccgacag
     2341 gcgtgcaatg cgcgcagcgt tagccgcaca cttattaata gtgtctgtaa acatttgatt
     2401 atcctttaaa aaataaaaac aaaaaaataa aacaaaaagt ttaaaaaatg tcctgcaata
     2461 taccgaaggt cattacagga ccatgcaaaa aggggaggca ttgcgcctcc cgttaaaaca
     2521 ttaaccgcga agctgcacca cgccgtcttt cactcgtgct tcgtaatgtt tgacggaaaa
     2581 ctgttcgtct tccatgcaca agccatcact taagcgaaaa cgctgttttt tcagcgggct
     2641 ggcgacccac agctcgccct ggtgttccgc aatcagcccg cgtgacagca cgctggactc
     2701 gaagaacggg tcgatgttgc tgatcgcaaa cacctgatcg ctgtgatacg ggcggaaaat
     2761 tgctacctgc tcgtcaccta acagcgcgca gacgccggtt tcaggcagga tgtcatcgat
     2821 tttgcagatg tctttccact ggctcatgcg ttgtcctcca ccagagttac cgggatacgt
     2881 tcatacggcg ttgccggacg gtgctgttcg cgctctggca ccatctgcac gttcgggtca
     2941 cgcttgtcgc tgttgatgaa gtgtttgaag cgagtctgcg cggacggtgt attgaccgtt
     3001 tcagtccact cacacactac cgcttcacgc aggcgcgcca tctcttcttc cagatgagcg
     3061 ttcagcccca gtttgtcgtc aatgatcacc gctttcaggt aatcgatgcc gccttcgagg
     3121 ttttctaacc acggcgcggt acgcgtcagt ttgtcggcgg tacggatgta gaacatcatg
     3181 aagcggtcga gatatttgat cagcgtttcg cgatcgatat ccgctgccag cagatccgca
     3241 tgacgcggtt tcatgccgcc gttaccgcaa acatacaggt tccagccttt ttcagtggcg
     3301 ataataccca cgtctttacc ctgagcttct gaacattcac gggtacagcc ggagacaccg
     3361 aacttcattt tgtgcggcgt acggatgcct ttgtagcggt tttccagttc cacgccgagg
     3421 ccgacgctat cgccaacacc gtagcggcac caggtgctac ccacgcaggt tttcgccata
     3481 cgcagtgctt tcgcataggc atgaccggtt tcgaagccag cttcaatcag ctgacgccag
     3541 atttccggca gatcgtcttt ctgtgcgcca aacatcgcca ggcgctggga gccggtgatt
     3601 ttggtgtaga gattaaattc acgcgcgata cgacccactg ccatcagccc ttccggggtg
     3661 atttcaccgc ccggagaacg cgggatcacc gagtaggtgc cgtctttctg gatgttagcg
     3721 aggaagttgt cgttagaatc ctgcagcgga gtatgttccg gcttcagaat gtattcgttc
     3781 cagcaggagg ccagcagcga accgacggtc ggtttacaaa cttcacaacc gtagcctttg
     3841 ccgtgtttcg ccagcagttc ttcgaaggtt ttaatgcctt caacgcggat caaatggaac
     3901 agttcctgac gcgaataagc aaagtgttcg cacaggttgt tgttaacttc gatgccctgt
     3961 ttcgccagtt ccgcgttcag tacctgagta accagcggga tacagccacc gcagccagta
     4021 cccgctttgg tttcagcttt cagcgccgca acggtgtggc agcctttgtt gatggcagca
     4081 atcagatcac ctttggtgac gtcgaagcag gagcagattt gcgcgctgtc cggcagttta
     4141 tcaacaccga tagacggctt gccgctacca gagtgtgctg gcaggatcag ggaatccggg
     4201 ttttccggca gttcgatagc gttcagcacc agttgcagca ggttaccgta gtcgctggta
     4261 tcgcccacca gtaccgcacc gagcagggtt ttgttgtctt cgctgacaat caggcgtttg
     4321 tagatctctt tactttcgtc gaggtaaacg tagctacgtg cgccaggcgt gcgaccgtgc
     4381 gcatcaccaa taccgcctac gtctacgccc agcagtttca gcttggcgct aaggtcagca
     4441 ccttcaaagg cgttttcgct acccagaata tggtcaacgg cgacctgcgc cattttgtag
     4501 cctggtgcta ccagaccaaa tacacggttg ttccagcttg cgcattcacc gatggcgtag
     4561 atatccggat cggaagtctg gcaggaatca ttaatgacaa tacccccacg cggagcaacg
     4621 tccagaccac actgggttgc cagcttatcg cgcggacgga taccggtaga gaagacgata
     4681 aagtcaactt ccagctcgct gccgtcggca aaacgcatgg ttttacgcgc ttcgacacct
     4741 tcctgcacaa tctcaagggt gtttttgctg gtgtgaacgc gcacgcccat actttcgatt
     4801 ttgcgacgca gctgctcgcc acccatctga tcaagctgtt ccgccatcag cataggggca
     4861 aattcgataa cgtgggtttc aatacctaag tttttcagcg cgccagcggc ttccagacct
     4921 aacaggccgc caccaacaac ggcaccgcgt ttgctgcgac gggcgcagga ttcaatggcg
     4981 ttgaggtctt caatggtgcg atagacaaag cagtcctcag tatcagaacc tttgattggc
     5041 gggatccacg ggtaggaacc ggtcgccatg atcagcttgt cataaaaaac ggtacgcccg
     5101 gcgctggagt gaatcacctt ctcctgacgg ttgatggtga tagcgcgttc gccgaccaga
     5161 actttgatgc cgtgtttctc gtagaagcct tcgcgcacca gcgacagctc ttcggcggtg
     5221 tggtgagaga agtaagacga gaggtgtacg cggtcataag cgatgcgcgg ttcttcacag
     5281 aaaacggtaa tatcaaagtt ggccgcatca gatttatcaa gaagatcttc gataaagcga
     5341 tggccgacca taccgttacc gataattgcg agtctgactt tgctcatttt tgcctcgatt
     5401 tcttttctat taccgcctac cttaacgatt cagcaacccc gcttattgat gtaaatcaaa
     5461 ttcaccttta tatactcctt aaggggtata ttgctgattt gtataaattt tcttaagtca
     5521 cggaattgtc tgatatttca tgtttgtgca aaaaatagac aaaatgaacg ggtttataga
     5581 aatagatgca cgtagcgggg gaagagagga aaaggccgga cgacgatagt gccgtccggc
     5641 attagcaatt aatgagaggt cagggtgtta tgctgacggt gacggctgac gaaacctaac
     5701 aggaagcaca tcacaaagac gacagcgtac agaccgtttg ctgtaagcag tgccgcctgc
     5761 ggaccgctat gttcaacaat cgggccggta accacaaagg tcaacatagt accgatggtt
     5821 ccgcaggtca ggacaaagtt aaccagtttt ggcgacggta ctttggtctg ctgtgaaccc
     5881 agggtgatga tggtggtata gatcgcgctg gagaagaagc ccagagcgag aattgaccac
     5941 gccatatgtg ctggtgttcc ggtgttaaag acgtacatca gaatcgcagc cagaccagcc
     6001 agtacggtca gaatgcgttg caaatcaaag aagcgaagaa taaagctgaa cgcccacatg
     6061 ccgaccatgt atgacatcca gaagttactc accagcgtgc ccgcgtcgtt caggctcatg
     6121 cccaggcctt tggcatactc aggcacccag gagataaaac ctaactgacc gaggatgtag
     6181 cacagcgccg caacagagag aaacagtacg ccgatccccc acttttcttt ctctaccgga
     6241 gcatccgttt ttggcgcatg tttgcccagc gccgggaact cacagccgaa ggtcagaata
     6301 aaaatagcga catacaccag cccgatgcag gcataaaccc agtaccactc aatgctgcgc
     6361 gccagtagaa acgcggcgat cattgggaaa atcatcccag ccatactgaa gaaggagtcg
     6421 gtaaataaca ggcgggaacc gcgctgacgc ccttcataca tttgtgttac caggaatgta
     6481 ccaatcgaca tggtgatgcc gctgaccacc ccgagaatga acatcgccgc cgagaacagc
     6541 gccaggctgt ggctgaacat caaaccggca accgccagca ccatcaggag aaagccaaaa
     6601 cgtaactgcg ttttcaacgg gacgatttcc atcagccagg cgttgaggaa gatagagatt
     6661 aaaatgccgg cgttgaggaa ggtgaaggtg ttactcatac tggaaacagg cagattgaaa
     6721 taatcggcga tatttcccat caccatcccg gtgacaataa ccaacgcacc agtcagtgcg
     6781 taggagagaa agctaatcca tgtgagcttg atgcgattgc tgttagtcat gattggcctg
     6841 cgttcaaaaa taaaatggca tagcgggata tgccgcgagc gggcgatttt aggtgatttt
     6901 gtgatctgtt taaatgtttt attgcaatcg gttgctaaat tgcattttaa gaggtgattt
     6961 tgatcacgga ataaaaagtg atcgtcaggt tacatatatt tcagatacgt aaaattaggt
     7021 aaagggatgg ccttgttctt gaaggctatt tagaatctct tcacttgctt tttttctgct
     7081 ctgtttgtta aggaaatctc atgttcaaat cgaccctggc ggcgatggct gctgttttcg
     7141 ctctttctgc tctttctccc gcagcaatgg cagcgaaagg ggacccgcac gtattgttga
     7201 caacctcagc tggtaacatc gaactggagc tggataaaca aaaagcgcca gtgtctgtgc
     7261 aaaactttgt cgattatgtg aacagcggtt tttataacaa cactaccttt caccgcgtca
     7321 ttcctggctt tatgattcag ggcggcggtt tcaccgagca gatgcagcag aaaaaaccaa
     7381 acccgccaat caaaaatgaa gccgataacg gcctgcgcaa cacgcgtggc accatcgcga
     7441 tggcgcgtac cgctgacaaa gacagcgcca ccagccagtt ctttatcaac gttgccgata
     7501 acgccttcct tgaccatggt cagcgtgatt tcggttacgc ggtatttggt aaagtggtga
     7561 aaggcatgga cgttgccgat aagatttccc aggtgccgac tcatgacgtt ggtccgtacc
     7621 agaatgtgcc gtcaaaaccg gtagttatcc tttccgctaa agtcctgc
//
LOCUS       DAZFXT010000188         7372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.188, whole genome shotgun sequence.
ACCESSION   DAZFXT010000188 DAZFXT010000000
VERSION     DAZFXT010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.188"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..458)
                     /locus_tag="ACHKW0_004576"
                     /pseudo
     CDS             complement(1..458)
                     /locus_tag="ACHKW0_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
     gene            complement(583..2583)
                     /locus_tag="ACHKW0_004577"
     CDS             complement(583..2583)
                     /locus_tag="ACHKW0_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HFO7477259.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRTLQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(2580..3716)
                     /locus_tag="ACHKW0_004578"
     CDS             complement(2580..3716)
                     /locus_tag="ACHKW0_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HFO7477260.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(3709..5912)
                     /locus_tag="ACHKW0_004579"
                     /pseudo
     CDS             complement(3709..5912)
                     /locus_tag="ACHKW0_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5682 family protein"
     gene            complement(5923..7011)
                     /locus_tag="ACHKW0_004580"
     CDS             complement(5923..7011)
                     /locus_tag="ACHKW0_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HFO7477261.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYTLLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYSEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         1720 a         1990 c         1947 g         1715 t
ORIGIN      
        1 ttcacttctt taaaaccagc ggctttcatc accagttcca tttgcgccat agtgatacct
       61 tttttggcat cttcagcaga aacgttgatt cctgaaatac cctgcagggc tttaaaatcc
      121 actttttcca tatcgatagt cacgttttcc tgcgcgtagg tatctgtata ggttaattat
      181 tcttcaacac ccgcgatgtt tttgtatttg gcgcttaacg gctcaagtgt cttggcagcg
      241 tcttctttgg tggttgcacc aatagaagca aattgaattt tggtttcaga agattgctta
      301 agcaccttgt cacctttgta gacataggta atggcaattt cagtgccgtt cagattggcg
      361 ctgaatttct tcgattcttc tttgtcaccg cagccagcaa gagagaaaac cagaacagat
      421 gcaacaacga gggaaaacag cttattgaaa gccttcatgt aaaactccat tttatttaat
      481 caagaaactg gtgactctca ccaggggcta tatagaatat gcctaatacc gtgacgtgag
      541 cagtccggaa ctggagtaga actcttagta aaaagcacta tttcatcctt gttgctgaag
      601 catggggaat aattgttcgc aaagtaaaac accgttattc attgcttcta cccgtgcctc
      661 gctttctgta ttacgaaatt gtcccaacac atgtgccagc cgataaaaac cgaccgcgga
      721 gaggtcattc gccagcaact ctgcctgact aatagcgctc tgttcctgat agcgccagcc
      781 gttatggagc agttgaataa gtaacgcctg gcagcgcatc agcaactgat gagcggtaga
      841 cggcacaggc aaaacgctgg cagaaggtag cggtgccaca ggcgcagttt ctgcgtccag
      901 cgcccaggcg cgggtttttg tcatcatcac ccgtggttcc agtgtcaatt gcccatcaac
      961 aaaactgaca aagccagaaa ccagactcac ggggtcgtct gtttgttgca aaagcgccgc
     1021 catgcgttca acggcaaaag gagaacaggc agatgccgga agggataacg tcaggagatt
     1081 atcttcacct tcgccgctga ttacctgcgc atccagcgtc tggcggctgc tatcccaacc
     1141 gagcgaaata cactcagcga ccggcagaat aaataagtta tcgacctgat taagaggccg
     1201 tatgcaggcg gggggacgct ggcgtaaata ttcccgcaaa gccacaatgc ccggctggcg
     1261 taacggcgcg ctcaacattt gccaggcatc aggcgacagc ggcacaacgc tgcttaagcg
     1321 gttgcgggta gctaacagca gctcgccatc ggcactgcgt tttgctgctt gtgaaacaat
     1381 ttgcccaccc gccagtgcgc cagcctgaaa actaaacagc cgacgcgtag ctgccggtga
     1441 gttttcctgt tcacttcgcg gccaactgcg cgaaaggtgc aaaatactgc cggtgtcggg
     1501 atcggtaaac cagatgcgta aaccataatg ctcaatatcc tgccagcaac gcatacctaa
     1561 agacaccagc cgcagatgat caagctttgc ttctccggca atgccagagc caacgaccgt
     1621 gcgccacggc acaggaggaa cttcaccaac actgtcgcgc cgggccatct cttgtgcgca
     1681 atttaatcga ctgtttaatg ccgcaagctg acgtaagcat tctccggcat gatagtggct
     1741 ggcgcgagtg tggaaggcat caacgcttgc ccgtagctgt cgtagtgatt cactcaccca
     1801 gcgccagttg caggcctctg ccgcctgcaa tgtgcggttg aatgctgcct cgtagtgaat
     1861 aagcggctgg ctgatgccgc caagccataa tgcctggctt aattgctgaa catattgacg
     1921 acacgtttgg ccttcttcgc tggcaaacgg atcgtcagat gatgtgacgt gttcgctgcg
     1981 catctgccag attaaatggt taaattctgc ttgctgcgct ttggcctcga cgaaggcctg
     2041 cacagccagt acgacatgtt cgcaaagtgt gccttcaata caatcacaac gggcgaaacg
     2101 aatactgcta cgggaataaa aacgcacatc gctcatcggt aagcgggcag agggaatttc
     2161 gcccggcgca cagaacaact caatggtgat gcctttagcg accagcgcct gcgcgcgttt
     2221 gcgggtggca tcgggcaggg tagccagttc ttccagccag attgccggat cccactcttc
     2281 ttctttttcc gtgggctgga cggtggcaca aagtcgttga taacttaaca ccagcatcac
     2341 gcgatgacgg cacatgccgc tggccccgca gctgcactga gcctctttca gtgcctggct
     2401 gttcgccagc tgggtacgga caccgtcact gaaggtggcg attaaagcgc cgttctcatg
     2461 gctgatttcc gggacgttgc cattttccag ttccttaaga ctgcgcttaa caaaaccggc
     2521 attgcttaac gccgtcagtg cctgcggtgt cagttctaat aattccggac gtagtgaatt
     2581 catgactgaa gattctccgc aagccatgat gccagctcgc ccggcgtcat ggcggctatt
     2641 tgtgcgccga cattaaccag cgcctgggcc gtatcgcggt catagcaagg cgttgcggtg
     2701 ctatcgagcg ctgccagtcc cagcactttg atgccgctct ggacacactt ttttacctga
     2761 tgcgtcagca atgatgatga acccccttcg taaaaatcgc tcacgaggat aatgacgctt
     2821 tttgctggtt gttcaataag ttgccgacca tactccacgg cactggcgat attggtcccg
     2881 ccgcccaact gtactttcat taataactct accggatcgg caacgtctgc cgtgagatca
     2941 accacgcttg tgtcaaacgc caccagatgg gtacgaatgc cgggtaactg ccacaaacag
     3001 gccgccatca ccgcagagtg gatcaccgag tccaccatcg atccgctttg atcaaccagt
     3061 aagaccagtt gccattgttc gctttggcgt ttaatgcggc tgttaaagcg gggggattcg
     3121 atatacaact tgccgtgttg cgggtgccag tgttgcaggt tggcgcgcag agtacttttg
     3181 aaatcaaagt ttcgcgccag tggaattaat gagcggcgac ggcgatcgcg gacaccagaa
     3241 aaagcctgac gaacttcctt tgccagtcgc gccataattt cttcaacaac ctggcgcact
     3301 atctggcggg cggcagccag tacttcggga ttcatcagat gtttggtgtg caaaacggcg
     3361 cgtagcaggc tttccgaagg ctgcatacgt tccagcacgt cgagattcgt caccacatct
     3421 tcaatgccgt agcgcagtac ggcatcgctt tccagtcgct caatcacctg ttgcggaaac
     3481 agagtgtgaa tactgttgat ccactcaggg gtggtgagat ttgagccacc taatccacca
     3541 gagcgttcac cacgctggag ccgttcagga tcgcgcccat acagccactc cagcgcgtgg
     3601 tctatctgcc gggcgttgtc atccagccca caaagcgtcg tttctgccgc ttcgccaaga
     3661 attaatcgcc agcgttgtag ctcacgggtg gtcagaagat cgttcagttc agacatggaa
     3721 aactccccag ttttgcagcg atgccagtgc ctgctgttcg agttgttgat gatgtgcaat
     3781 ggcctgtggt ggacaatgca acggcatttg cagcgccgaa acgggaagtt gcgccagttg
     3841 ataatgctca agcacctgat gcgccagcgt cccgcgttct cgtggtggta gccaggccat
     3901 cgccgcgcgt aaatcgggca gggcattgat aaaatcggca tcacttagtt gatttaaatg
     3961 actgctgaaa ccggcgataa atgtcggctg acaggccagt tgatggcggg ccagcgccag
     4021 caaaccgtgc agcgcttcgc cggattgtgc cggggataac tgcgccagca tcgtcagcgc
     4081 cgcttcggca ctggcgttag gatgttccag acgcatcagt gcgccatgag ccgcgccgcg
     4141 atccagcgcc ggggcatgaa ttgcctgact gcgtcgctcc agtaaggcta ccgccgcaga
     4201 aagcgaaacg ccggataaat taacgccgct atgtagatcg cgcagaatat ggcaaagcgc
     4261 ttgccagcta tgcaggtgag cgtgaaactc cttttcatcc ggtctgccgt tagattcaca
     4321 cagccacagc gtgcgatcga tagccgcgca taacgtcgtt tgtaatatct gcgcgccttg
     4381 cataccgcta atttcatcca gccgccataa ggcatatagc acttccagcg cctggcccat
     4441 ttcggcaaat tgattttcct gggcgattaa taatgtgagt tgctccagta attgttgact
     4501 gaaggacgcc agacccgcta acgccgcctg gctaagacat gtggtgatac tgccgattcc
     4561 acccgcgtcc agcatatcgg cttctaattt atggcgtgcg gcttcctgga gtgtggcacc
     4621 aaagcaggcg gcctcaatca atgccgcatg ctggctaagc gggcgggtta atttccagtg
     4681 ttcttcaccg ttgcctgcaa gtgtcagtgt gcttccctgc tggcgcacaa tccctgggat
     4741 ctccagtatt gccagccgat gtaacacctg actttgcgct agtccattgg ggttaaagcg
     4801 atttaaggta agctccgccg gaagggagat tgcggtacgt tccagttcgc aggtgacatc
     4861 ttttggcaga ggaggttgtg gcgtagaagg ggcaagttta ccgaatccgt cacccgctaa
     4921 tgtgtctatc aacgttagca gaatcggatc ggtgtcggga tgaataacgc cgcgatagct
     4981 ccacggcaac ggcgcattca gggcttcttt aatcagcgat cctgctatgg catccagcca
     5041 gtcagtgcgt aacggtagtg tatgaccgcg caactgtgcc agcgccatcg catgcagatg
     5101 agcggcagcc atatccgcgg tcgaagcagg caacttgtgc tggcgcaaac gggtaaggat
     5161 agtttttagc agttgttcac cggcctgctg taagccccac tgccagcacc agttttgcca
     5221 gaccggggca ggcataccgg aaaggtatcc tgccagcaca tcaaggcgct tttcactgta
     5281 gggcgtgaga taacaacctg taatggcatc tgccagcgag ggcaattccg gcgtgttaat
     5341 gtcctgcggg cattcgcgcc acatttttgc cagtgccgga gcgtgccagc caccgcagac
     5401 gactaacacg tcgccattat tttgctgcac cgcccatgca atccagcgag ccataaaggc
     5461 ttcgcgctga cgattgagtg catcgccggg gaaatcgccc cgcagttggg caaaatagtg
     5521 cgccagcgca gagggtaatg cagtttgctg gctttcatct tgcgtatcag gcgaatcgtc
     5581 ctcttcttca ctctgcgccc agcaaggcaa atcgatgaag taagtttgcg cctgaatacg
     5641 acgtgcggct tgtagcgcct gccactccgg cgaaaattca gcaaatggcg tccaggcacc
     5701 acgcccgggg gctgcaccgt cctgatactg gcaataactg taaatagcta ccggaagctg
     5761 gtgggaaaaa aaagttcgtc tacccggtca ttaaaatcag ccgggccttc aatcaacacg
     5821 tatcgtggcc gctggctttc gattaacgat ttcaccagcc gggcgcaggc cggactatga
     5881 tgccggatgc cgacaattaa cggctcgctc atgcctcttt cctcacggca ggcggtggcg
     5941 ggcttgataa taagcctgcc agtgagcttc tttatgtgtg gcgacacgct gttcggagta
     6001 acggcgcaga cgagcgcgat cttcttcgtt atctttgacg atggttccgg caatacagtc
     6061 caccaggtcg gcaggctccc ccgcgcgatt cgctaaaaac caggcgcgga cgcctacggc
     6121 gtgggccacg ttcacggctt cagcggtgga cataatcgct gtcagtgtat ccattgacgt
     6181 tttcttttcg ccgttggcac gcagatcgcg gaaggtgcgt accagtaact ccagtactgc
     6241 gtcaggcact ttatgcggaa taccgctatg ggccagcaaa cgcgccgaag cactggcgac
     6301 cagttccagc tcctgggcga aatccataat cggaaagacg gtttcaaaat cgaagcgacg
     6361 ctttagcgcg gcactcatct cattgacgcc gcgatcgcgg gtattggcag tggcaataat
     6421 attaaagcct tcccgcgcat agagctgact ggcttcaccg gtgagttccg gcaccgtcat
     6481 caccctgtca gaaagcatac ctaacagaca gtcttgtact tccagcggtg tacgcgttat
     6541 ctcttcaaaa cggacgattt tgccgtcgcg cattccctgg taaagcgggg cagggaccag
     6601 tgcttccgtt gacggaccat ggttgataag cagggtgtag ttccagccgt acttgatttg
     6661 atcttcggta gtagatgcgc cgccctggat ggttaacccg gcatcgccgc taatagcggt
     6721 tgccagtaat tcagacagga gagattttgc ggtgccaggt tcccccacca acatcaaacc
     6781 gcgcccggtg gcaagagtga ctaacatgcg ttcgacagcg gagggactaa tcaccacttt
     6841 acggctaata ttttgcgcgc tatcgccaag gataaaagca cgggccgcag gcaaacttaa
     6901 ctgccaaccg ggcgggcaag gtgcgttatc attttgtttt aattttgcca gttcatcggc
     6961 gtataacaca gcagcaggcg gacgctgcag atgattattc tgcggtgaca tatattcatc
     7021 cctgaaatgt attaatgaaa taaacgatat tttacgcgca ataaatgttt ggcgcattag
     7081 ttgtttgagc gaaaaatatc tggaaagcag atcacagtaa ataaaaaaac ctgctggtga
     7141 ggcaggttct tttatttact actttcaggc ctaacaccac agcctgtgca acatcgtgtt
     7201 ccaacgaacg aattataacc acggctggac cccgttgcaa ttaagataat gagatttata
     7261 atccagaccg ttgggacgtt gcgccggatc gaatctattt caagtcctgg ctaccggacg
     7321 ggtggtgctg caggtggtgc cctgatccgg agtgcaacat cgtggtccaa ca
//
LOCUS       DAZFXT010000189         7352 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.189, whole genome shotgun sequence.
ACCESSION   DAZFXT010000189 DAZFXT010000000
VERSION     DAZFXT010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7352
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.189"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(10..1020)
                     /gene="pyrD"
                     /locus_tag="ACHKW0_004581"
     CDS             complement(10..1020)
                     /gene="pyrD"
                     /locus_tag="ACHKW0_004581"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HFO7477262.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            complement(1131..1868)
                     /locus_tag="ACHKW0_004582"
     CDS             complement(1131..1868)
                     /locus_tag="ACHKW0_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HFO7477263.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            complement(1834..2349)
                     /locus_tag="ACHKW0_004583"
     CDS             complement(1834..2349)
                     /locus_tag="ACHKW0_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7477264.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            complement(2357..2899)
                     /locus_tag="ACHKW0_004584"
     CDS             complement(2357..2899)
                     /locus_tag="ACHKW0_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7477265.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            complement(2911..3981)
                     /locus_tag="ACHKW0_004585"
     CDS             complement(2911..3981)
                     /locus_tag="ACHKW0_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7477266.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            complement(3972..6572)
                     /locus_tag="ACHKW0_004586"
     CDS             complement(3972..6572)
                     /locus_tag="ACHKW0_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HFO7477267.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQACVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGANSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQATLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDVTQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            complement(6597..7298)
                     /locus_tag="ACHKW0_004587"
     CDS             complement(6597..7298)
                     /locus_tag="ACHKW0_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0061077 - chaperone-mediated protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HFO7477268.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
BASE COUNT         1874 a         1880 c         1642 g         1956 t
ORIGIN      
        1 aagaaataat tagatatggg taacgatttc tttaatcagc ggcggacctt taaaaataaa
       61 accagaataa atttgcacca gtgaggcacc cgcagcaatc ttttcacgcg cagcgataac
      121 cgagtcgatg ccgccaacac cgatgatcgg taagcgaccg tttaattcca gtgacaagcg
      181 gcgaataatt tcggtgcttt ttaactgaag cggacgacca cttaagccac cggtttgatc
      241 gcaatttttc attccctgaa caagagaacg atcgagtgtg gtattggttg caataacgcc
      301 atcaatatta tggcgaacta aactatcggc aacctggatc aattcttctt cagaaagatc
      361 cggcgcgatc ttcactgcga tcggcacata tttatggtgc atcgcttgca aatcattttg
      421 cttattttta atcgcggtta agagatcatc cagcgcttca ccatattgca gcgtgcgtaa
      481 tcctggggta ttcggcgatg aaatattgat ggcgatatat cccgcatagg catagatttt
      541 ttccatacaa atcagatagt catctttgcc ctgctccact ggcgtatctt tatttttgcc
      601 gatgttaata cccaggacgc cgtcataatg ggcctttttt acgttctcta cgaggttatc
      661 aacgccaaga ttattaaagc ccatacggtt gatcaaacct tcggcatcta ccagacgaaa
      721 gagacgcggc ttgtcattac ctggctgtgg acgtggcgtg acggtaccga tctcgatcga
      781 tccaaatccc atcgcgccta acgcgtcaat gcactccccg tctttatcaa gaccggctgc
      841 cagaccaagc ggatttttaa acgtcaggcc catgcagtta acaggtttcg caggcacttt
      901 ctgccgcacc agtgcttcaa acggcgttcc tgtaatacgg cgtaattgct gaaaagtaaa
      961 ctcatgagcg cgctctggat cgagctggaa aagggcttta cgaacgaagg ggtagtacat
     1021 gaactctcct ggattcccgg tgtgcaaacc gggggcgtat tatgtgcgag ttcggaccaa
     1081 aagggaattg acctgcggca aaaaaaacgc aaacgttttc cgaacctgtt tcagggcaga
     1141 ttttgctcct tctttgcact caacccgccg taatcattta tcgcccgcca ggcgacatga
     1201 cacggtgcga ttgcctgaca ccagcttgtc tgacgctggc ttttaggcgg cacaactccc
     1261 gcattactta acgctttacc atttacgctc aactgaatca ggttaatgta gtaaggcgtt
     1321 gggttagtga gttgtacacc ctgcgaattc cgtgtccagc gtaattgttg ataagcttcc
     1381 agcgggtcgc ccggcaaacc ttcgggtcgc cagaacagtt taaataccga gcgcatcgcc
     1441 acttttacgc tctgattttc aactttgccg gatggcacgc tggcaatgct taactcaaat
     1501 agcgtttcgc gatccacagg caagatgtcg ctttccgttc tcagcaagcg caacgtgcct
     1561 gtagtaccgg gcttcaggag aataagtggc ggagcggcca gtaatggtgc tggcactgtc
     1621 gacgcttccc ccccggccca acgcgttggg cggttgattt tactgttaat aagccaggat
     1681 tcctgactgg tattagtcag taaaatagat atcgattctc tgtctgccgg aaagataaat
     1741 cgcgttccgc cgaccaccac tcctgcccgc aattcacccg ccaccagcag cgataaaacg
     1801 gcgaaaatca agagcgccaa tctgttccac gtattagtca taatgaagta caaatgtcgc
     1861 gctggcattc actttacccg actgcacatc gctattgaca ggtcgcaatt gcgcataaaa
     1921 agttaatggc accttaccgc tatgtaggct gtagtcttta ctcgcctggt taagcggaat
     1981 caatatcttc gcatcatcca gaatcgcaat tgccagcccg ctggctgcat ccgtaccact
     2041 ttcaatggac agcatttcgc tctgcactcc gtcgggggtt cctgaaaagg tgacgtcaac
     2101 cgtgctgaca tctgttccgc aatcctgcaa attaatgatg aaagatttct ccgggccata
     2161 attcccatga aaaaaacgtg tcagcgagac atcgcctaac tgcacattga tttgcttgct
     2221 atcctgtgcc agcacgcatg tattacggcg aaacgatccg ctaatgttga ctgtgccatc
     2281 atgggcaaat agctcacagc cccccgttaa tcccagtaag aacagaatcc agattatccg
     2341 tttcaacatc actccttcac tggtaaacca gatcaaaata gagaaccgcc gtcgcattac
     2401 cgcccgttgc tcccgccttt gtggacttat aacgaagttg atatttgagt gtgttatccc
     2461 cggcttttaa gggcgtaaga ggctggacct gattgagcgg gatttcttgc tgactttgcg
     2521 catcaagaat ttgcacggca atccccgttg ccataccacc gcttccgcct gtgtcagtta
     2581 gtttgagcag tgtcggcgca agggtgtcag cctcgccgct aaaaaggacg tccgcgccag
     2641 taataccagc ggcacaaccg gtgatatcga tattcaaatc agcagcagtg cttacggtat
     2701 tcgcggcctg aaaatcactg atattaaaat caccaatatg aatattttgt aatgcacttt
     2761 tggtggcgac atcacagccc tgatcgacca cattcccata aatatgcata ctaacttgtt
     2821 caggcccagc catcccgaca tggatgttaa gaatgctaaa gaacaagata acgcactgaa
     2881 atatcgtttt tttcttcata cgttcacgcg ttaatcaaaa tttattctca ccgttgccgt
     2941 ggcttcaaat cgcccggttt ctggtggtac acctgtcgtg ctgactggat agacattcat
     3001 tcgcgcgata ctgtctgcat catcaggaat agagagcggt aatgttccac cagcagggga
     3061 aataatgttg ttctggctat cggtcaccac cacgcccacg ccaggacgtg acgtcttaat
     3121 cgcctgggga tagctggggt ctgtggttgc cgtcagactc aggttgacgt taactaaccc
     3181 cgcgtttccc gtgttacacc tgactggaat tgcaagttct gccgttctgg ccccaagagg
     3241 cttatgccct acaacacgaa agttagccgc aaatacgtcg cccagatcga cctcgattgt
     3301 ttctccggca ttgacggaac atgagccaag tgaattgata gtaccagaca ggtaatacac
     3361 taaaaccggt gtacctgtag tcgtgcagga atcacctgcg ggtatgttgt agcattcaaa
     3421 taatcgcgcc agcgccatag gaggaatgac gaccgaacca acgaacggct gcaatatttt
     3481 cagggaaaag ctaccgctat taccgctagc cccctggcca cctaaacgtt gaccgccggg
     3541 atcgcaaata tgccatcgtg tatctgcccc tgtgcccacg ttttcgaaag gaaccgcagt
     3601 aggagaactg cctttaacct gcaataaaac tttggcggca agatagtcat taattttgta
     3661 ccagttgtcg cctaaataga ctaacggtga atccgcggcg aatgcccatc ggccacttct
     3721 gacattgtct ttatcgcaat cacaggaaac ataataatca ttcgattcgt tccactgata
     3781 aaatttgtct ttaactactc cagccgtatt ttcttcttcc tccagggttt taacgccaaa
     3841 attgacgcca atatacggat tcccctgagt ggtatgacac tgcccgctat aagcagcagt
     3901 ttgagcgcac catagcatta agacggcggc aaaaaatagt cgtagtaata acacgcattt
     3961 ttctccaaag attatctgca tatggcatcc attcgcgtaa tcgcagagtt aatttgttgt
     4021 tcggtaagct ggtaactgat atgacaaatt tgatcgggag cttcgcccca gcgtacagtt
     4081 atcgctccct gagcgggcaa accggacata tagagcgagc catcatcatc aataatgccg
     4141 ctattagtct gtatgccgtc cagcgtcgct atcgcgccaa aacgcagcgg tataccctgc
     4201 tttgatgttt tcattaatac ccttgctccc gcatgggcat tgaactccgc cagaaccaac
     4261 gcccctttgg tgggaaccac gttgactact gcattttcaa gatccacgtt acgttttaac
     4321 gatgccgcat caagtgcgat tcgattttcc cgataagaag tggcataggg gacaacggta
     4381 tatccgcgcc agtcagtttt cagcccggtg ctgttcgcca cacgagtatt ctctgcgcct
     4441 ggtgctgcaa tcaggacgtt agtttccccc agcgattgcc ccagggtaat gccctgtgaa
     4501 tgggcaacta aactgcctga aagagcgtag ttcagttgtt gttgactgcc gttatcgctg
     4561 tagttgtagc ccactcgggc atctgcaaac gcccctttgt agtccatgct ggcgctacca
     4621 ttagccgttt tcccctcgct gttatatccc tgttgcacgc tgtagctcag gttaccgtca
     4681 tcgagtagtg tctccgttaa acccaggttt tgtgtaatgt gcccttcgtt atcgatactg
     4741 gtgttagagg tcatccaggc gtaatttttt ttcctggtat agctatcatt acttctgggg
     4801 agcaacagat tgagcggcag ggaaaaattt agtgcaaaca cctgatcagc atcaggttga
     4861 ccacgggact tactgtagtt ccaggaaacc cccagcgaga gatctttaat ttgcgtgttg
     4921 tagccaaact gtaatagccg atcctgttga tcggtatgcc agtaagtttg ctggctacca
     4981 cttaaataaa acgaaccgta ctcgcctaat tgctgggaga tattgacctg cagtttgcca
     5041 cgtttggtgt aaaacaaatt gtaataacgc gaccacatcg gcgtatcttc atcatcaccg
     5101 tcattaaatt cgtagccatc catatgttta tacatggtgt cggaaagggt gtagaaaccc
     5161 gacgtcgaat agcgatagcc catcaattga aaattagtgc ccaattggtt gagcgttttg
     5221 gcatacagaa aacgatatga ctgaccttta tgttcgcttt ggtctgccag cgtactcttc
     5281 gcctgggtca catcgaacga aattgctccg aaatcgccaa ggttaaatcc cagaccaaac
     5341 atggcggcac ggtaatattc agcatattgt ccaccaccat accatgtcgt tccccacggt
     5401 ccgccccact gcaacgtggc ctgggcaaat ttgctctcct gctgttcatt actattggtt
     5461 ctgtatttcg ccagtgtcac cgcgtatttg attcgcccct gacgctggag taatggcacg
     5521 ctggaaaacg gtacgctgta gctattgacg ctgccgtccg cttctttgat ttcaactaac
     5581 aaatcaccgc tcgacgacgt ggaatagaga tcactaattt caaaagcacc aggcgataca
     5641 taagtttggt aaatggtgta accattttgc ttaatcgtta tctgggcatt gcttttggcg
     5701 ataccacgca ccacaggcgc aaaccctttc aggctgtctg gcagcatgtt gtcatctgac
     5761 gccagctgca caccacgaaa gctgacacta tcgaaaaaat cgcctgcagt ataatcatca
     5821 cctacggtca gttcgccttt tagtggaata accgcccgct gcaaatagct gctgagattc
     5881 ttccattctg cggtttggcc actactgcga ctccatgttg aattgttgcg caatctccat
     5941 gggcctaaat taacgccact gtttaaattc agaaaatagc tgtcgccaga atcactgtct
     6001 gcgctgctat gaatactgtt agccccgcta aacgaatatc ccagtaatag cgcgttgatc
     6061 ccttcatccc aacgttcagg ggggacataa ccacgggcat tacttttgat ggcgatttgc
     6121 ggtactgaaa tttccagacg gagtttattc acattaaacg ttacgctggc atccggaata
     6181 atctccgcca atggcacaca cgcttgtttg tccagttgtt taaaagccgg aaacgcatca
     6241 gtattaacac ccattgcatc caggctttca gtcgtcaaac aggcggcaat ccccttctct
     6301 ggcgttatct caacaaaatt gacgttgcgc gtatctacaa ttgtctgatt cagaacaata
     6361 tcgacacgat aaacccctgg cgttattttt tgtccttttt cgaaacgaga caaatcggcc
     6421 accattgagg gatcatcttt gaaaaaagca ggatcgaacc aggtttcagc ttgtgcattg
     6481 aacgctgccg acacaaacac caccagccca ccaataaacg atggcactcc accagagctt
     6541 aacaggctgt gttgtcgatg agttctatac atctcgctct cttaactgaa gacggcttac
     6601 gatttacagt tgagttgcgg cgtttccgcc ccgtagtcat tgatggttcg atacgttacg
     6661 ctgctatttt tggccccctt tggcagagaa aattggtatt tatcaaaggg ggaaatcatt
     6721 tgatttttaa ctacttttcc acctattttc aggtctgtca gggtaatgaa ataaggtgtt
     6781 gggttactga ccgtaaccat gtcagctgac gaagtgcaac gcagtttcgc cggagcctcg
     6841 cctaagggat acgctaaacc ttttggtcgc cagaaaagtt tgatgacgct ttgtgtggca
     6901 atcttcaggg tatttccctc ttcccgcttc gttgttgaag ggactgcccg tacgctagtg
     6961 aagaaaaggg tttctctgtc tttcgccaac ggcgcaccaa tgtacataat gcgtaacaga
     7021 ttctcattag ccggattcag cacaaatagc ggtggtgtaa taataaaatc tgctgatttt
     7081 ttattatctg gggtcatcac ccatgactga ataagataag gaacatccgg actggtgtta
     7141 cgtacaccca gtgaaatttg ctcttcacct tctgaataaa caagacgtgt tgctgcaagt
     7201 ccaatcccgc ctttacctgc cgcccatgcg gatgagcagc tgagtaatat tgccgttaaa
     7261 cttacggtca caattccctt tgttatgcaa gttttcataa atagtcactc ttaaaaaata
     7321 tgaatatccc ctccagaaca ctagaggggt ac
//
LOCUS       DAZFXT010000190         7288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.190, whole genome shotgun sequence.
ACCESSION   DAZFXT010000190 DAZFXT010000000
VERSION     DAZFXT010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.190"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(164..526)
                     /locus_tag="ACHKW0_004588"
     CDS             complement(164..526)
                     /locus_tag="ACHKW0_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HFO7477269.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            811..1020
                     /gene="yibT"
                     /locus_tag="ACHKW0_004589"
     CDS             811..1020
                     /gene="yibT"
                     /locus_tag="ACHKW0_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibT"
                     /protein_id="HFO7477270.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            complement(1032..1619)
                     /gene="mtlR"
                     /locus_tag="ACHKW0_004590"
     CDS             complement(1032..1619)
                     /gene="mtlR"
                     /locus_tag="ACHKW0_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HFO7477271.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(1619..2767)
                     /gene="mtlD"
                     /locus_tag="ACHKW0_004591"
     CDS             complement(1619..2767)
                     /gene="mtlD"
                     /locus_tag="ACHKW0_004591"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008926 - mannitol-1-phosphate
                     5-dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0019594 - mannitol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HFO7477272.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVDAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            complement(2862..4775)
                     /gene="mtlA"
                     /locus_tag="ACHKW0_004592"
     CDS             complement(2862..4775)
                     /gene="mtlA"
                     /locus_tag="ACHKW0_004592"
                     /EC_number="2.7.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HFO7477273.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            5312..5674
                     /locus_tag="ACHKW0_004593"
     CDS             5312..5674
                     /locus_tag="ACHKW0_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7477274.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            5677..6813
                     /locus_tag="ACHKW0_004594"
     CDS             5677..6813
                     /locus_tag="ACHKW0_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7477275.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAVALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT         1776 a         1826 c         1808 g         1878 t
ORIGIN      
        1 tatttcggta aaaatgcatt ttcagaatat caaccagcgg taaatcgaag taaagggagt
       61 gaattgtttg gtaattaggc gagtagacag gagagtcccc gcattgacca gctaaggcaa
      121 gccagatgcg ggttgatatg atcgaaccag tcgtctgagg cgcttaaaac gcagtcttag
      181 agaatccagt catctcctgc aggcccattt cacggcccag cgcggtcatt gggtgcacaa
      241 ccaccagtcc gcgaacactt ttcttcagct tgcccatatc agcctgctct tttttggtaa
      301 ttgcgcgctg aaacggcatc ttcatcagct tctgtgcttc tttactcagt ttttggctat
      361 gaacttcacg caggcgagca atttctgctt ccagcgtggc tttctctttt tccagctcgg
      421 catatttgtc tgcggcttca accagcgaca gatcggcctg ctggtggcgg atggcgtcca
      481 gacgatcgct gagacgtttg atttcgtttt tttcgacttc tttcatgtga tggcttctga
      541 taaagataaa tttatagccc ctagtatgcg cgaccaggtg ctttgttcac aactgatgac
      601 attgcaatag ttctgtaacg taatcattaa catgcttaaa aactacgcaa tttattgatt
      661 aattaagaaa tctatagaaa aaagataata aacagcagcg gatgattatt gaggattcag
      721 cacgttttcc ttaatgtcat ctttaccggg aacctaaact aaaattggcg gccgcgcatt
      781 cccggcgcgg ggtaatacgg agatatcatc atgggcaaat taggtgaaaa cgttccgctt
      841 cttatcgata aagccgtaga tttcatggca tcaagccagg cgttccggga gtatctgaaa
      901 aaacttcctc cccgtaacgc gattccgtcc ggaatacccg atgaaagcgt gccgttatat
      961 ctacaacgtc tggagtatta tcgtcggctt tatcggccga agcaggtaga ggggcagtaa
     1021 gcagacgttg cttacttttg aaaggctttt tttaagctga ttctggaaat cagctcagtc
     1081 agtgaaagga ccattgttga tcgcacagcc tgttgatagc gctgaatttg cattgcatac
     1141 aaactggagt ctgctggttc aaactgtggc ggcggtggta acgccgccac gcagtgcagt
     1201 tcaccaaagg gtccaaggat ctcgtcgtcg gtaaaggcgt actcgttgcc gtcgtgattt
     1261 agttcttcac gcaatgccat cagcagctcc gcatcttcgt attcctggcg gttaatgacg
     1321 cccaacccgt aaatgagttt taaacgcaca gaaagatcgc ccagcggacc gtcgccgtcg
     1381 agtaacggtt ctacagcata cttcaccgcg taatcgtctt tgcggaatac ctgaagcacc
     1441 agaagattta ccgcctcggt caaaagctcg acggcggtga tcagaaagct tcgcacggtt
     1501 ttgccagcat tcagacgctc aagcacacgg ttttcaaagg cctgggtttg ttccattgtt
     1561 gcctgcatat ctgacaatct gttattcggg cgcagggtgt cctgcgcctg gtccaccatt
     1621 attgcattgc tttataagcg gttaccgcct cggatacaac ctcgctgttg gcatcaagac
     1681 cggaaatctg tgccagcgcc gcctgcggac ctttgtcagc gatcagtgct gccagttcct
     1741 gagcctgcgg atcatcttca ctgcggaagt gcattgcacc agcaatcccc tgaatcagat
     1801 tcttgtgcgg aaggctgtat tccagcgtac cgagcagtgg cttgatcaga cggtcgccag
     1861 cactcagttt acgcagcggc tgacggccta cgcgctctac atcatctttc agatacgggt
     1921 tctcaaaacg accgaggatt ttctggatgt acgccgcatg tttgtctgcg tcaaagccgt
     1981 agcgcttgat cagtaccgca ccactttctt ccatcgcacc ttttaccacc gcgcggattt
     2041 tctcgtcgag aatagcgtca cgaatggtct gatgaccagc cagttttccg aggtacgcgg
     2101 ttatagcatg acccgtgttc agggtgaaga gtttacgttc gacaaatgcc atcaggttgt
     2161 cggttaactc catgcctggg atgttcggca gtgcgccttt gaactgcgtt ttatcgacaa
     2221 tccattcgct gaaagtttct accgtcactt ccagcggatc gttagttgcc gaagccgaag
     2281 gcggtacgat gcggtcaacg gcggaatcga caaagccaac gtgttcttct acccacgctt
     2341 tggcgtcttc cggcagggcg ttcatcacat ggcctttcag ctgcgtggta ccgcgtacca
     2401 tgttttcaca ggcgatgatg ttcagcgggg attcattacc ttgttcttta cgtttcacca
     2461 gccctttggc gatagccgga gcaatacgtt ccagcacaac cgggccaacg gcggtagtga
     2521 ctaaatcgac ctgagcaatc agatcaacga catcatcacc aatgctgctg acagcatcga
     2581 cgccggaaac ggtatctacc tgctcggttt caccaaccac atgaacctga tagctatgac
     2641 gggcattcag ggcatcaagt accacctgat tgacatcggc aaacgtcagt tggatacccg
     2701 cgtctgccag cagtttaccg ataaagccac gaccgatatt acctgcgcca aaatgtaatg
     2761 ctttcatagt attaaccttc atcaatgttt ttacccgaga gggctggggt gaggcttttc
     2821 cctcacccaa accctctccg tgaggagagg gtaggattgg attacttacg acctgccaac
     2881 agttccagca cttcatccac gctggtggtg tgtgccagac gctcgatgac ggactcatca
     2941 tccagtgcat tggtcaggct ggtgataacc tgaatgtgct cgttgttacg ggctgcaata
     3001 ccaatcacca gacgggcaat gtcatcttct tcttcaccga agcgcacgcc ttccgggtac
     3061 tggcagaaca ccacgcccgt tttcagtacg cgatctttcg cttcaaccgt accgtgtggc
     3121 accgcgatag actcacccag ataagtcggg gtcagttttt cacgatccag catcgcctga
     3181 acgtattccg gctcaacgta accgcctttc accagctgct cgccagcaaa acgaatcgct
     3241 tcttctttgg ttgctgcttt gcgaccgagg aagatgttct ccgcgcccag tttgaacagg
     3301 ttagcactgg aatcgtcaaa gctgtctttc agactgtctt tcactttcac ttcgttttca
     3361 gtatggcgct gggcagcaac cagacgttcg gttaggctgg tgtacaggcc gctgtcgagg
     3421 aagttggtca gcgaaatatg ctgcgcctgc ggaacctggc gcatagcgcg ttcggtcagg
     3481 tcacggtgag tgatgacgag gtccacatct ggcggcaggt tgttgatcgc gctgttagtg
     3541 accgaaatct gtgacagacc tgcatcctga attttcttac gcagcacgcc tgcgcccatc
     3601 gcactggaac ccataccggc gtcacaggca acgatgattt tacgtacgtg gctcaggtcg
     3661 ttagtcacat cgcccgcaga cagcggagat gtgcctttag actcagcttt catgtcctgc
     3721 atacgacgag ttgctgcttc aatatcatct tcttctttca ctttgctggt tttcagcaaa
     3781 atagcagaga caacgaagga gacagccatc gccgcacaca cacccgcgat gttagcgaaa
     3841 taagcacctt ttggtgtcat cgccagtaca gcaaggatag aacccggaga tgccggagaa
     3901 accagaccac cgcccaggat agtcagcgtg aacacgccag tcataccgcc gaggatgact
     3961 gcgaggatca gacgcggatt catcagcaca tacgggaagt agatttcgtg gatacccccc
     4021 aggaagtgga tgattgccgc accgcccgca gactgtttag cgctaccacg accaaagaac
     4081 atgtacgcca gcagcacgcc catacctgga cctgggttag cttcaatcag gaagaagatt
     4141 gatttaccca gttcatggga ctgctgaata cccagcggcg agaagatacc gtggttaatg
     4201 gcgttgttga ggaacaggat tttcgccggt tcaacaaaga tagacgccag cggcagcatg
     4261 tcatggacaa ccatgaagtt aacgcccgca gccagcattt tggacagggc ttcaacaatc
     4321 gggccaatgc cgaggaatgc cagaatagcg aggatcatcc cgatgatgcc tgcggagaag
     4381 ttattcacca gcatctcaaa accggatttg atcttaccgt ctacccagcg gtcgaagtgc
     4441 ttaatgcacc agccgcccag cggacctgca atcatagaac cgaggaacat cggcatgtct
     4501 gcgccgacga taacgcccat ggtggtgatg gcaccgacta cgccgccacg ttcgccgcct
     4561 accagcttac caccggtata accgatcagc agcggcagga gataagtgat catcggcccg
     4621 accagcttcg ccagcgtctc gttcggtaac caccctgttg gaataaataa cgcggtgatg
     4681 ataccccacg cgataaacgc gccgatattt ggcatcacca tgttgctgag gaaacgacca
     4741 aagctttgca ctttgatctt aatatcggat gacataaaaa caccccttct tatgtttgct
     4801 gtcgcgcagg ctggaagccc gaggtttttt gttaatgtgg cggcagaggt agccggacct
     4861 tgtagatact gcgaaatctg gcactgaatc ggttaactgt ccagtcgacg gccttttgtg
     4921 tgatatcaat cacataaata tagggggtag agaggtaatt gacgtgacgt tcatcacaaa
     4981 acgcttgtgt aaaaaagcaa caaggcggcg attttcgcta aaaatggcat ttaaatgtga
     5041 gtagtgtcac atttttgttt tgatggtttg ttgaatcttt gtgatctgaa tcacaagata
     5101 ttttcttacg gcgttcacct gatgtgatct acagcatgtt atgccttcgt catacaatcg
     5161 ttatgtaact aagcaactca tcacctctaa aaattattct ttggtgttat ggcgagaatc
     5221 gcgaacccgg attattgtca aaatatcagc ctgactccct tctggctgcc agcccaaaaa
     5281 caacactgat aaacgtgaat ccgaggcaga tatgtttcta aactatttcg cgttgggagt
     5341 gctgatcttc gtctttctgg tgatttttta tggaatcatc gcgatacatg acatccctta
     5401 tctgattgcc aaaaagcgca accatcccca tgccgacgct attcatacgg cgggctgggt
     5461 gagcctgttt actctgcatg ttatctggcc gtttctgtgg atctgggcga cgctctatca
     5521 accggagcgt ggctggggta tgcagtcaca tgttgcgtcg caggagaaag cgactgaccc
     5581 ggaaatcgcc gcactttctg accgaatttc ccggctggag catcaactcg ccgccgagaa
     5641 aaagactgac tattccacgt tcccggagat ctaattatgg atctattgat tgttttaact
     5701 tacgtggcgc tggcgtgggc ggtctttaaa atcttccgca ttccggtaaa tcagtggacg
     5761 ctggcgacgg cggcgctggg aggcgtgttt ctggtgagtg gtttgatttt gttgatgaac
     5821 tacaaccacc cttacacttt taccgcgcaa aaggcagtga tagcgatccc tatcacgcca
     5881 caggtgacgg gaattgttac tgaagtcact gacaagaata atcagcttat tcaaaagggc
     5941 gaggtgcttt ttaagctcga cccggttcgt taccaggcgc gagttgacag acttcaggct
     6001 gacctgatga cggcgacgca taatataaag acgctgcgtg cgcagctcac tgaagcgcag
     6061 gccaacacca cccaggtttc agcggagcgc gaccgtctgt ttaaaaatta tcaacgttac
     6121 ttgaaaggca gccaggcggc ggtgaatccg ttctcggaac gtgacatcga cgatgcgcgg
     6181 caaaatttcc tcgcgcagga tgcgctggtg aaaggctcgg tggcggagca ggcgcagatc
     6241 cagagccagc tcgacagtat ggttaacggc gagcaatcgc agattgtgag cttaagagcg
     6301 caacttactg aagcaaaata taaccttgag cagactgtca ttcgcgcgcc gagcaatggc
     6361 tacgttactc aggtactgat ccgcccaggt acatacgcag ttgccttgcc gctgcgtccg
     6421 gtgatggtct tcatccccga gcaaaaacgg caaattgtcg cccaatttcg gcaaaactcg
     6481 ctgttacgtc tgaaacccgg cgatgatgcg gaagtggtgt ttaacgcgct acctgggcag
     6541 gtgtttcacg gcaaactgac tagtatttta cctgtcgtgc caggcggttc ttatcaggcg
     6601 cagggggtat tgcaatcatt aacggtcgtg cccggcacgg acggtgtgct gggaaccatt
     6661 gaactggacc ctaacgatga tatcgatgcc ttacccgacg gcatctacgc ccaggtggcg
     6721 gtttactccg accatttcag ccatgtttcg gtgatgcgga aagtgctgct aagaatgacc
     6781 agctggatgc attatcttta tttggatcat tgagggggag gggggagatt atggaacttt
     6841 gctccttgta aggactaatt atttttgcat attacttcat tagctgcgat gatttttctt
     6901 taatattcat tatgttaata agttctggat gagtgtgctc tcattggatt ttttatccgt
     6961 cttgttctgc cgcaggatga gactatacat cgtcgagatc gcaatgatga tatgagttgt
     7021 tcctcctgcg acggctctaa tcaaaaaaga gcaggagtgc ttttccatct attgcgatga
     7081 tggctttatt atctgaagga tgacagtcac atttaaaaaa tatttaatct atagatcaca
     7141 atgtagggtc atggaatata tgtatacaac tattttcttc ttcactttta attatcgtat
     7201 tcattataaa actaagttaa aaaaactcct ggagagtgtg ttattaatat atttttaaat
     7261 aaaattaagt tatcattcat cgatgcac
//
LOCUS       DAZFXT010000191         7191 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.191, whole genome shotgun sequence.
ACCESSION   DAZFXT010000191 DAZFXT010000000
VERSION     DAZFXT010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7191)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7191)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7191
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.191"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..527)
                     /locus_tag="ACHKW0_004595"
     CDS             complement(<1..527)
                     /locus_tag="ACHKW0_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar nucleotidyltransferase"
                     /protein_id="HFO7477276.1"
                     /translation="MKGIILAGGSGTRLYPITMGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIQDILIITTPEDQQGFMRLLGDGSQFGINLTYAIQPSPDGLAQAFIIGENFIGNDTV
                     CLMLGDNIFFGQGFTPKLRDAAERTSGATVFGYQVMDPERFGVVEFDRNFKALSIEEK
                     PANPKSNWAVTGLYF"
     gene            complement(524..1600)
                     /gene="rfbB"
                     /locus_tag="ACHKW0_004596"
     CDS             complement(524..1600)
                     /gene="rfbB"
                     /locus_tag="ACHKW0_004596"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HFO7477277.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSKRYVFEHADICDAAAMARIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALDSDKKNSFRFHHISTDEVYGDLPHPDEVNNTEELPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDAEKIGRELGWKPQETFESGIRKTINWY
                     LNNNDWCRRVQDGSYQRERLGVIR"
     gene            complement(1972..2865)
                     /gene="galF"
                     /locus_tag="ACHKW0_004597"
     CDS             complement(1972..2865)
                     /gene="galF"
                     /locus_tag="ACHKW0_004597"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HFO7477278.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLELRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(3040..4434)
                     /gene="wcaM"
                     /locus_tag="ACHKW0_004598"
     CDS             complement(3040..4434)
                     /gene="wcaM"
                     /locus_tag="ACHKW0_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HFO7477279.1"
                     /translation="MPFKKLSRRTFLTASSALAFLHTPFARALPARPSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGLVCDNINIGIFIPAGKTLHILGSLRGNGKGRFVLQDGS
                     QVTGEEGGSMHNITLDVRGSDCTIKGLTMTGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHIIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRVNHHIV
                     NVEKINFRLPERRE"
     gene            complement(4445..5665)
                     /gene="wcaL"
                     /locus_tag="ACHKW0_004599"
     CDS             complement(4445..5665)
                     /gene="wcaL"
                     /locus_tag="ACHKW0_004599"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HFO7477280.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGLVATPFHADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(5662..6942)
                     /gene="wcaK"
                     /locus_tag="ACHKW0_004600"
     CDS             complement(5662..6942)
                     /gene="wcaK"
                     /locus_tag="ACHKW0_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HFO7477281.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCNALILRESVSLELMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFSTPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARHNEAVSRERHTGMQMVQSVLERIGEVK"
BASE COUNT         1779 a         1802 c         1685 g         1925 t
ORIGIN      
        1 taaaaatata atcctgtgac agcccaattt gattttggat tggcaggttt ttcttcaata
       61 cttaaagctt taaaattcct atcaaactcc actactccaa atctctcagg atccattact
      121 tgatatccaa agactgtggc acctgatgtt ctttctgctg catcccggag tttgggtgta
      181 aaaccttgtc caaaaaaaat gttatctcca agcataaggc atactgtgtc atttccaata
      241 aagttttcac caataataaa tgcttgggct aaaccatctg gactaggttg aattgcatat
      301 gtcagattaa taccaaattg gcttccatcc ccaagtaatc tcatgaaccc ttgctgatct
      361 tctggcgttg taataataag aatatcttgt atacctgcca acatcagcac cgacaaagga
      421 taataaatca tcggtttatc ataaatgggt aatagctgct ttgaaactcc cattgtaatt
      481 ggataaagcc ttgttccaga accaccagct aaaataattc ctttcatcta attaccccca
      541 aacgctcacg ttgataagag ccatcttgaa ctcgccggca ccaatcatta ttattcaaat
      601 accagttaat tgttttacga atcccgctct caaacgtttc ctgtggtttc catcccaatt
      661 cgcgaccaat cttctcagca tcaatggcat aacggcgatc gtgtcccgga cgatcggcaa
      721 cataagtaat ttgctcgcgg taagatttct ctttcggtac aatctcatcc aacaaatcac
      781 aaatagtgag cactacatcg atgtttttct tttcgttgtg tccaccaatg ttataagttt
      841 cacccgcttt accttcggtt acgacggtat ataacgcacg tgcatgatct tcaacataca
      901 gccagtcgcg aatttgatcc cctttaccat aaataggtaa tgccttacct tccagagcat
      961 taagaattac tagtggaatc aatttttccg gaaagtgata aggaccgtag ttattcgaac
     1021 agttagtcac aatggtcggt aaaccatagg tacgtttcca cgcacggact aaatgatcgc
     1081 tagatgcttt tgatgcggaa taagggctgc ttggcgcgta agctgttgtc tcagtaaata
     1141 agggtaattc ttctgtatta tttacctcgt caggatgagg caaatcacca tatacttcgt
     1201 cagtagaaat atgatgaaaa cggaagctat ttttcttgtc gctatcaaga gcagaccagt
     1261 aattgcgagc ggcttccaaa aggacataag taccaacaat attggtttca ataaatgccg
     1321 cagggcctgt aattgaacga tccacatggc tttcagcagc caggtgcatc actgcatccg
     1381 gctgatgctg agcaaaaatc cgcgccattg cagcagcatc gcaaatatcc gcatgttcaa
     1441 aaacatagcg tttagagtca gaaacatctg caagtgattc caggtttccg gcgtacgtta
     1501 atttatcgac attaacaaca ctatcctgcg tattatttat aatgtgacga actacagcag
     1561 aaccaataaa tcctgcgcca ccagtaacaa gtatcttcac tttattattc catattactt
     1621 cagagcatgc tgtgaaataa gcggctctca gtttgattaa tagaggtatt aatgcacgct
     1681 accgcccctg gctttacagc taccagagca ctgcatacat gtccacgatg tgacgagcgt
     1741 tacccactcg cgctaaaccc gaaaaattca aacgctaatt gtcttaccaa tccgctctgg
     1801 aaacaaggaa aatcctggaa aactttgact aaaatcctat tgctaactcg ttgttattct
     1861 gattgtttat ataaaacaac ggcaggaaaa ttcgcaacaa attactttcg ccacgaatct
     1921 tcactgccgt aataattttc ttatcaaccg ttacattcgg tcagattttc attattcgct
     1981 taacagcttc tcaatccctt tacggaactt cgccccttct ttcaggttgc gcagcccata
     2041 cttcacgaac gcctgcatat agcccatttt tttaccgcag tcgtagctgt cacctgtcat
     2101 cagcatggca tcaacggact gttttttcgc cagttcagcg atggcatcag tcagctgaat
     2161 acgcccccat gcaccaggct gagtgcgttc aagttccggc caaatatcgg cagaaagcac
     2221 atagcgacca acggccatga tgtctgagtc caacgtctgc ggctgatccg gcttttcgat
     2281 aaattcaaca atgcggctga ctttaccttc acggtccagt ggttctttgg tctggatgac
     2341 cgagtattca gagaggtcac ccggcatacg ttttgccagc acctggctac gacccgtttc
     2401 gttgaatcgt gcaatcatgg cagcaaggtt gtagcgcagc gggtcggcgc tggcgtcgtc
     2461 gatcacaacg tctggcagca ccacgacaaa tggattgtca ccaatggcag gccgcgcaca
     2521 caaaatggag tgacctaaac ctaaaggttc gccctgacgc acgttcataa tggtcacgcc
     2581 cggcggacag atggactgca cttccgccag cagctgacgc ttcacgcgca gttcaagaag
     2641 agattctaat tcataagagg tgtcgaagtg gttttcgacc gcgttcttgg acgcgtgagt
     2701 taccaggagg atttctttga tccctgcagc cacaatctcg tcaacaatgt actgaatcat
     2761 tggcttgtcg acgatcggta gcatctcttt gggaatcgcc ttagtggcag gcaacatatg
     2821 catcccaaga cccgctacag gaataactgc ttttaaattc gtcattattt catccacctg
     2881 taaaatggtt gctgaattat agcttgttcg atttttttcg ccagcatcaa ttaccctgaa
     2941 ttgattactg aattacttgt gatgttacgc cgcttcgttg tggattgcag tagcattgtt
     3001 cctaagtatg gctccatttt tccaggaatg gtcgcaaatc tactctctcc gttccggcaa
     3061 tctaaagtta atcttttcca cattaacaat atggtgatta accctatcga tatcgacgga
     3121 gctttgtcct ttctcattca ccgcatgaac atttgcaaga gatagcaggg tttctttttt
     3181 cgccataaag acgccgcgaa cgtctttgcg catgtcaaag ttcatgctca atgcgggtcc
     3241 aacagaggat tcctgcatca ctttgatatt acgcataaaa agatgttgcg gtttgttgtg
     3301 taactccaga gacgcacgct tcatctcaat gttagtcagt gccacaaagg agacggcatt
     3361 cccggcagag atttggatac cgcgcaattt gtaagcaagg tgggtattat ccagttgaat
     3421 attattcact cggaaatttt gtggtatcga gagatatttg cctttaatta ccccatagcc
     3481 gattaacatt ccggcactat taatcatttc aatattatca atcacgaaat tatcacaacc
     3541 gtaaatagcg actgtcgcgt tatcaatacc tgctttctta ctgaaatccg gcgtgatatt
     3601 gcgggctttg atattacgga taacaaaatg tttaccattt tcaacatgga tcaactgccg
     3661 acaatccgat cccgtgatat tcgccacgac aaagtttttc actgcctggt cttccgggta
     3721 attattatca taagtgcttc ccgcaagacc tatgccaatg ccccagttga ttttgccgtt
     3781 agtacagttg atgcgctcga tgatgtggtc ggagatcaaa atatcactgt cgttaattgc
     3841 cacgttccat tcaatggcgt cgccttgtaa gtcgctgaac ttacagttgg tgatgttggc
     3901 accgataatc tggttatgaa atccctggcg taagatggcg taattagcgt ggctgacagt
     3961 gaggttatcg atggtcaagt tacgcatgac ccgtttgttt ttgccgccga tatagatctg
     4021 cgtcaccggg ccaaagccgg tcatcgtcag ccctttgatg gtgcagtcag aaccacgcac
     4081 atccagggtg atgttatgca tactaccgcc ctcctcccct gtcacctggc tgccgtcctg
     4141 taaaacaaat cgccctttgc cgttgccgcg taggcttcca aggatgtgta acgttttacc
     4201 tgcaggaatg aagatgccga tgttgatatt gtcacaaacc aatccggcag gcacgacgac
     4261 cgtttgccct tcactaaaag cttgtttaaa tgaggcgatc cagtcgtgcg gattgtaatc
     4321 gttgatatta acgcttggtc gagcaggaag tgcgcgagcg aaaggggtat ggaggaaggc
     4381 aagcgccgag cttgccgtca ggaaggtgcg tcgggagagt tttttaaatg gcatacgttc
     4441 tcctctataa agcctgcagc aagctggcga gttctcggtt aatcacctgc tggttaaaat
     4501 cgtgttcgac tttttcgcgc gcacgtttga cgaccggagc cagttcgtcg gtgtccagtt
     4561 ggctaaacgc cgccagtcgt tgcgccagtg cgcgagcatc gttctcaggc accagccagc
     4621 cggatttatc ggcctccacc aattccggta ttccgctatg cagagtagaa accaccggaa
     4681 tgccgaccgc catcgcttcc atcagcgcta ccggaatgcc ttccatatcg ccatccgcgc
     4741 ccgttaccga tggcaacagg aagacatccg cgtcgtcgag catcgctttc acttcgtggc
     4801 tcggtttaaa gcccggcatc tctaccacat cttccagttg atattgttcg atgagggtac
     4861 gcaggcgtcg ttcccacggg ccaatgccga ggatgcgata gcgaaatgcc atgccctgct
     4921 ctttcaactg acggcaggct tcgatcgcca catgcaggcc ttttttttcg gttaagcgtg
     4981 cgacggagat gatttccagc ggtgttgcgg gcgctttcac gggacgcggg ctaaagcgcg
     5041 tcatatctac acccatgcgc gatacggcga ttttttccct cgggcagccc attttttgca
     5101 gccttccggc ccacagatcg cttatcggta gcatcaggtc gccacgtcga aacagttgtt
     5161 gatattcggg agtgtaatgg ttgagcactt cccgactgga aatatcaatg ccgtggaaaa
     5221 tagtggcaat tttgccgcga atgacaccca gttcgcggag ttttgctgcg gttacccccg
     5281 ccgggccaaa atgagcgata aagacatcgg catgaaacgg tgttgcgacc aggccgcaaa
     5341 tggcagacaa aatcaggttt cgcgactcag caccatagcg tttgaggttg agcgcctgcc
     5401 aggtattttt acgatgaatg ccgcgtaagg tctggctggc gcggtggcgc agtttcgcaa
     5461 ctttgcccgt aggttcgtcc tgtaaccagc gggttctggc ggcaaggttg tatttcgtcc
     5521 atgccgcgtg ggtgttttgt gtgtcgcctt tttgcagcgc gacaatctcc acctcaaagc
     5581 ccatatcaat aaacgcggta atttgattga ggacgaaggt ttccgacgac agcggaaatt
     5641 tcaataagaa gaagccgacc ttcatttcac ctccccgatg cgctcaagca cagactgcac
     5701 catctgcatt cctgtatgac gctcacgact gacagcttcg ttatgtcgcg cgttcagcgc
     5761 cggaagctgg cctaaggtat ccgcaaccat cgcttgcagg ctgccgtcta ataaatgacg
     5821 gatatcaatt gccatctccg gtagtcccag ctgctgcata atcccggcgg atttatgttc
     5881 gtagttgatg gcaattgccg gagtggagaa attcatcgag ataatggcag agtgcaggcg
     5941 cgtaccgacg gtgagttcac aggcaccgag aattttgccc atttccagat cgttgagttc
     6001 atccatcact acgtgataac gggcaggatc gctgatgtgc tggcgcaggt tgagcgccac
     6061 catgcggtcg tctttgttat agctgtcgat gcccgtacag gtggagagcg caatcacctg
     6121 atacccttcg tcgagaatgc gattgaccac cccggcaaag gctttttcat acgcttgctg
     6181 agtggtgccg aggcgtttgt cgaacggtgc cagttcgcgc agggtaattg ccaccgtttt
     6241 ctgttgtgcg gcaacgtcca gccagtgttg gacggcatag ctggcggtga aatcttcggt
     6301 gtggtgatcg accagccacg cggtatcgac gccatgttcc acttttgcag tggtgatatt
     6361 gctgcgtttc atcagttcga gactgaccga ttcgcgcagg atcagcgcgt tacagtgacc
     6421 aaaaacgtag ttcgccagtt ggttaaattg ctcatcctgg aagggaccga cgctgtgacc
     6481 aatcataaac agcggctttt tcgccataaa tgtgcaaagc gcatgttcaa actgcggcac
     6541 gccgtagaga tcgacaaaaa acgatccgcc gacctgaata atggcgtcgt aacctgagag
     6601 caggcgcacg aagtcggtga atccctgggc gatggcgata ttgcgcagct tgccagtgtc
     6661 ggtgacgcgt gagagcaata cctggtgctg atagcggcga cggaggactt ttttaacgcg
     6721 cccgacaacg cccgccgcgc tgttgtgttg tttcatttgc aggaacagcg gatcgcccat
     6781 taccgggcgg ttgagcagcc aggaagaact gaccggatag cggctcatca catccacttc
     6841 ggcgtgtgga ttgaggatgt tgatggcatc aagtaagccg cgcaggatgg cgctgtcgcc
     6901 acgattgccg caagtgtggt tgcccagaat aagtaatttc attttttcct cataaatttg
     6961 atgccaggtg aggctgtgtt tgttgtgttc gttgccgttt tttgcctgat gcgacgctgg
     7021 cgcgtcttat caggcctaca agacccgagc acagaaccgt aggccggata aggcgtttta
     7081 cgccgcatcc ggcattcagt gcctgatgcg acgctggcgc gtcttatcag gcctacaagt
     7141 cccgagcaca gaaccgtagg atggataagg cgttttacgc tgcatccggc a
//
LOCUS       DAZFXT010000192         7101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.192, whole genome shotgun sequence.
ACCESSION   DAZFXT010000192 DAZFXT010000000
VERSION     DAZFXT010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.192"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..911
                     /locus_tag="ACHKW0_004601"
     CDS             <1..911
                     /locus_tag="ACHKW0_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO7477282.1"
                     /translation="VMLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAESGDI
                     DAQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVRVDYA
                     KAYALFNQAAQHGVNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLAT
                     CRKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFS
                     IRRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRT
                     TIVAATATINGKDVELLKAGAIEQKW"
     gene            complement(967..1509)
                     /locus_tag="ACHKW0_004602"
     CDS             complement(967..1509)
                     /locus_tag="ACHKW0_004602"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HFO7477283.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKQGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1493..1804)
                     /locus_tag="ACHKW0_004603"
     CDS             complement(1493..1804)
                     /locus_tag="ACHKW0_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HFO7477284.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(1989..2879)
                     /gene="cyoE"
                     /locus_tag="ACHKW0_004604"
     CDS             complement(1989..2879)
                     /gene="cyoE"
                     /locus_tag="ACHKW0_004604"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HFO7477285.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2891..3220)
                     /locus_tag="ACHKW0_004605"
     CDS             complement(2891..3220)
                     /locus_tag="ACHKW0_004605"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HFO7477286.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3220..3834)
                     /locus_tag="ACHKW0_004606"
     CDS             complement(3220..3834)
                     /locus_tag="ACHKW0_004606"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HFO7477287.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3824..5815)
                     /gene="cyoB"
                     /locus_tag="ACHKW0_004607"
     CDS             complement(3824..5815)
                     /gene="cyoB"
                     /locus_tag="ACHKW0_004607"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HFO7477288.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5837..6784)
                     /gene="cyoA"
                     /locus_tag="ACHKW0_004608"
     CDS             complement(5837..6784)
                     /gene="cyoA"
                     /locus_tag="ACHKW0_004608"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HFO7477289.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
BASE COUNT         1935 a         1766 c         1771 g         1629 t
ORIGIN      
        1 ctgtaatgct gatagtttca ccaagcgtgg tggccagcga gaaagcgcat gaattgtatg
       61 acagcatata cggtggtaaa cctgctcccg atgttataaa tacactgcat aaaatggctg
      121 aatcgggaga tatcgacgcg cagagcctgt taggctggga atattatcag cctcgttatg
      181 ataccaaacc tgatgttcag gaagcgatta aatggttcga gttagcggct aaacaaggtg
      241 acagagaagc tccgttagcg ctggggggta tctactacga cggtgagcag gtgcgggtgg
      301 attatgccaa agcgtatgca ctatttaatc aggccgcgca gcacggtgtg aatttagcat
      361 ggtccaggtt gggcattatg tacgccaatg gtcagtatgt tgaggtagat tgtaagaaag
      421 cgaaggaata tcttgataaa ggtgtccaca tttatggtgg cccggaagac tttctggcta
      481 cttgtcgaaa agacatgatt gacagaaaaa cggttgacga tacgttaccc gtgattacgg
      541 ttacccgttc tgggatgcga gataattttt tagataaagg tttttcctgc atggatagcc
      601 tcttcgccac caccaataaa ttaggcgaag tggccaatct tcgcgtgaca ttcagcattc
      661 gtcgcccatc cggtaaggag attaaccaaa cggttggctt cgcgcctttc ggtttaaacc
      721 ggctgaatat tagctttact gattacctat ttggttcatt taccagtaat tcgtctctta
      781 ttctttataa accggaattt gagcgaaaat cttgtgcaac tgtaaggacc acaatcgttg
      841 ccgcaacggc aaccattaac ggcaaggatg tggagttgct gaaagcgggg gcaattgaac
      901 aaaagtggta acgctgctca gcccccggct gaggcaaaac ttgccctggc gggtggcgat
      961 aataatctaa gccgcgagaa tatgctgaat cgccaaaaac ataggtacag caccaaaagc
     1021 atttggtgtc gctgaaagct gaacaaagcc gagacgagca taaaaattaa tggcggcagg
     1081 gtcagcatca agataaaccc ctttaattgg taatgcctgg tgaattattt ttacatgttc
     1141 aaaaaaatca catagtaggt cctgaccaaa gccctgcttt tgatacttcc gtgctacccc
     1201 caacatgact aatctgacaa caccaatttc tgaaggttgt gaaccctgaa ggacgccaga
     1261 aacgcgttgt ttttccagcg aatatgccgt aaaagtacag atgccgatca gttcaccact
     1321 ttgtctgtca ataagtgctt tagccgcaca gtcgctatta cgcacacttt tctttagcga
     1381 tgcgcgtaca aatttatcga taacagggtt accgcaatca aactgttttt gcccaggata
     1441 agtaatatca gactgataat tacaggagag gactatgggc agagtaatct cttcatgctt
     1501 gtcgaccatc gctgtttttc cttttcatta acgccttgag ggcgagcgtc ggttgagcag
     1561 gttcagcgat gagttggttc atcagttccc agctttgatt cgaaagctca cggcgacgtt
     1621 ggttatcaag cacactttcg gcgcgttcca tagcagcgct caaaataaaa gcgcttaaat
     1681 ctaccccaac ggcttgcgca gcttcacgaa gtttgttttt ggcgtcggga ctagttttga
     1741 gttcgacccg cgcatttttc agcgttttat cgacatcggg tagagtggct gtttttccag
     1801 gcattatgac ctctttgatt aactgaatga aatcgtaggg agtcgatctc atacataata
     1861 cggctatcaa ccgtatgatt aatttatgtt catctgctca ctctgtcaag acatacggtt
     1921 attagccgta tctctgatct cagacaataa aaaaggtgcc gtagcacctt tttaatagag
     1981 aggttttgtt accacacagc agccagcagc gtatgcgagt ccggtaccat aaaatcaacg
     2041 gacatcatca ccgagagggc agtgatggcg atgatagaga agccgaacag cttgcgcgcc
     2101 cagattctgt catcagcaac tttataaccg cgcagagcca tacctaacca ccagacgcta
     2161 accgccgcgg cgaccaccag atatttatat ccagcgtaac cgccaagaga gagcatcagc
     2221 gtggcaacgg caaaggcgat gatatacagc gtgatgtgat tcttcgccac cgaaatgcct
     2281 tttaccactg gcaataccgg aatgtttgcc gcctggtaat ccttaaagcg gaaaatggcg
     2341 atggcatagg agtgaggcat ctgccacagg ctgaagatag ccagcaggat cgctgcgccg
     2401 ctatcgaact cgccggttac tgcacagtag ccaatcaccg gcggcgcagc gccggagagc
     2461 gaaccaatca atgtgccgta gactgagtgg cgtttcatgt acaggctata gacgccgaca
     2521 taaaccacaa agcccatcac ccccagccag caggccagcg gattcgcgcc aaaccacagc
     2581 agcataaagc cagcaatacc cagcaaggtg gcgtacacca gcgagacagc aggagagatc
     2641 aggcctttaa ccagcacccg attcttcgtc ctttccatct ttctgtcgat atccctgtcg
     2701 atgtagttgt taaacacaca acccgacgcc acaaccagtg acaccccaac cagcgtgtag
     2761 ataaacaggg gataatcaat gctgcccttt gaggccagca ggaatccccc aatcaccgag
     2821 atcaggttgc caaagatgat gcctggtttc gttacttgca ggtattgctt aaacatcata
     2881 accgccgctc ttagtgcatc atcatgttgt agttgaggtt ccacataatc cagatggagc
     2941 ctacaaccag gatagcgatg attagcacgg tgaagacaaa cgccgtcatg ttccagcctt
     3001 catctgattt ggtattcatg tgcaggaagc acaccagatg caccagaacc tgtactactg
     3061 ccattgccag gattgttccc agaattacgg ccggagaggc agcccctgtc atcaccatcc
     3121 agaacggaat caccgtcaga atgatcgaca ggataaagcc tgtcatgtag gtttttacgc
     3181 tgccatggga cgcgccgctg tgatcggtag aatgactcat tacatcgccc ccatcagata
     3241 aacaacagtg aacacacaga tccaaaccac atccaggaag tgccagaaca ggctcaggca
     3301 catgatgcgg gtacggttag tgctggtcag gccgcgacga gcgatttgta ccatcagcac
     3361 cgccatccag ataagaccgg aagtgacgtg cagaccgtgc gtgccgacca gcgcaaagaa
     3421 cgctgacagg aagccgctgc gatccggacc catgccgtta acaatcaggt gatggaattc
     3481 gtagatttcc atcccgataa atccggcacc aaacaaccag gtcaacgcca gccaggagat
     3541 aacctggctt ttgttgtttt tgtacatggc gatagccgcc atgccgtagg tgatggagct
     3601 gaacaacagc aagaaagttt caaccagaac gaacggcagt tcgaaaatgt ccttacctgt
     3661 cgggccgcct gcggtgccgt tcaccagaac ggcataggta gcaaacaaga ttgagaacag
     3721 aatgcagtcg ctcatcaggt agatccaaaa tccgaagatt ttggttccgc ctgcatcgtg
     3781 gtgcccgtgt tcgtgcgcgt gggcagtcgc gtgcgtcaaa gtatcagttg ccatttttca
     3841 gccctgcctt agtaatctca tcgaaatgct ggttttccag tttttcgatt tctgccaccg
     3901 gcacgtagta atccacgtcc tcgtcgaagc ttttcacgat ccaggtgatg atcatgcctg
     3961 cgaagccaac aatcgccagc caccagatat gccagatcat ggcgaaaccg aagatggtgg
     4021 agaaagctgc aatgacgata cctgcaccgc tgtttttcgg catatgaatt tcttcatagt
     4081 ggtcaggctt tttatacgct tcgcctttct ctttcatttc ccagaatgca tcacgctcgt
     4141 gaacgtgcgg cacaacggca aagttataga acggaggcgg ggaagaggtt gcccactcca
     4201 gcgtacggcc accccacggg tcgccagtca ggtcacggtt ctggtcgcgg tcgcgaatag
     4261 aaacgtacat ctgaataacg aggcagagaa tacccagcgc aatcagtact gcaccgctag
     4321 ctgcaatcat cagcatggtg tggaactgcg ggtcaatctg ctggctcaaa cgacgggtca
     4381 tgcccatgaa gcccagcgca tacagcggca taaaggcaac gaagaagccg atgatccaga
     4441 accagaacgc gcgtttaccc caggtttcat tcagtttgaa accgaaggct ttaggccacc
     4501 agtaggtcat ccctgcgaag cagccgaaga ccacgccgcc gatgatcacg ttatggaagt
     4561 gcgcaatcag gaacaggctg ttatgcagaa cgaagtccgc gcccggtact gccagcagca
     4621 cgccagtcat cccgcccacc gagaaagtga cgataaaacc gatggtccac agcatcgcag
     4681 aatggaacac gatgcggccc tgatacatgg tgaacagcca gttgaagatc ttcaccccgg
     4741 tcgggatggc gataatcatt gtggtgatac caaagaaggc gtttacgttc gcgcccgcac
     4801 ccatcgtaaa gaagtggtgc agccaaacga tgaacgacag cacggtgata cagacggttg
     4861 cccataccag cgaggtataa ccaaacagac gtttacgcga gaaggttgcc gcaatttcgg
     4921 agaacacacc gaaaacaggc aggatcagga tgtaaacttc cgggtggccc caggcccaaa
     4981 tcaggttgat gtacatcatc atgttgccac ccatatcgtt ggtaaagaaa tgggtgccca
     5041 gatagcgatc cagggtcaac aacgcgacgg taaccgtcag aattgggaag gaagcaataa
     5101 tcagtacgtt cgcgcacagt gatgcccagg taaatactgg catcttgaac atggtcatgc
     5161 ccggtgcgcg catcttcaga atggtaacga agaagttgat accggtaagc gtcgtaccta
     5221 taccggatag ctggagactc catatccagt aatcgacacc gactcccgga ctgtactcta
     5281 ttcccgatag cggtggatag gccagccagc cggtctgcgc aaattcgccc acgccgagag
     5341 aaacgttaac cagaatcaca ccaacaacag taaaccagaa gcttaagttg ttgaggaacg
     5401 ggaacgcaac gtcacgcgcg ccgatctgca gcggaaccac caggttcatc agaccgataa
     5461 cgaaaggcat cgccacgaag aagatcataa tcacgccgtg cgcggtgaag atctgatcgt
     5521 agtggtgagg tggcaggaag cccgcttcgc ccgccgaggc aagagcctgc tggctacgca
     5581 tcataatggc gtcagcaaaa ccacgcagca acatcacaat cgccacgatg atatacatga
     5641 taccgaggcg tttatggtcg acggaggtca gccactcttt ccacaggtag gtccacttac
     5701 cgaagtaagt gatcaggcca acgagcgcca gacctcccaa aataatgcca gcgatcgtaa
     5761 ccatgacgat aggttcatgg aacgggactg catcaagtga taattttccg aacatcttta
     5821 ttcttcctca acccctttaa tgggcggatt ccgcgtggct catgtccatg ccttccatac
     5881 cttcgtgtgc actgtgctca ccttctggct gggtcatgtc catgctctta ccgtgagcca
     5941 taaacttgtt aatcacatcg gcaaacaagt ctggtttcac gttggagaaa tattccacct
     6001 ggttgtattc gctaggcgcg gccagttttt cgaacgcagc catgtcagac atggtgttcg
     6061 gcgactgttt cgcttttgcg acccattggt cgaattcggc gcgatccggt gttgcaatag
     6121 ctttgaactt catgcctgag aagcccggcc cgctgtagct ggcggagata ccgtcataag
     6181 taccgggttc gttggcgatc agatgcaggc gagtctgcat accggccatg gcataaatct
     6241 ggctacccag acgcggaatg aagaaggagt tcatcacgga gttggaggtc actttgaagt
     6301 acaccggagt gttcgccggg aaagcgattt cattcacggt agcaatgccc tgttccgggt
     6361 agatgaagaa ccatttccag tccatggaaa ccacttcgat ggtaatgggc ttctcgtcgt
     6421 gtgccagcgg cttgctaggc tcaagagcgt gagtggtttt ccaggtcagt accgcaagga
     6481 agatgatgat taagataggt accgtccaga ccacagcttc cactttattg gagtgtgacc
     6541 agttcgggct gtacttagca tctttattgc tcgcacgata cttccaggcg aaaccaacag
     6601 ccatcaagat tgcgggaata acgacaatca acatcaggcc aaatgccgtc agtatcagtg
     6661 aacgttgctc cagaccaatc tgtcctttgg gatctaacag cgcagaatta cagccactga
     6721 gcaatacagt gcctgcaaat aatgacaacc atcccaaact tttattgtat ttcctgagtc
     6781 tcatttaacg acctcaattc cacgggatct ggtggcgttt aaagtgtgtg ggcattttac
     6841 gggaaggtta cattactgta aacatgatta aattcctgtt acttggattt ggctgtcagg
     6901 tcacatatgt tgcaaaacat atcaagttga ttttaagaag ctgttgcaaa agggcgttat
     6961 aacgaaaggg gagtgcagaa ggagtttgga cgggcaattg gtataaccaa tctggaataa
     7021 aacaattgtt taacaaataa ttatcattat ggtgacaatt gggtgggggg aaaataaaga
     7081 ctcattagct gaatcgtgta a
//
LOCUS       DAZFXT010000193         7100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.193, whole genome shotgun sequence.
ACCESSION   DAZFXT010000193 DAZFXT010000000
VERSION     DAZFXT010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.193"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            105..1439
                     /gene="adeQ"
                     /locus_tag="ACHKW0_004609"
                     /gene_synonym="yicO"
     CDS             105..1439
                     /gene="adeQ"
                     /locus_tag="ACHKW0_004609"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HFO7477290.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1492..1944
                     /gene="yicN"
                     /locus_tag="ACHKW0_004610"
     CDS             1492..1944
                     /gene="yicN"
                     /locus_tag="ACHKW0_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HFO7477291.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2155..3345
                     /gene="nepI"
                     /locus_tag="ACHKW0_004611"
     CDS             2155..3345
                     /gene="nepI"
                     /locus_tag="ACHKW0_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015211 - purine nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015860 - purine nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HFO7477292.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFSVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3386..3679)
                     /gene="yicS"
                     /locus_tag="ACHKW0_004612"
     CDS             complement(3386..3679)
                     /gene="yicS"
                     /locus_tag="ACHKW0_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YicS"
                     /protein_id="HFO7477293.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3901..4719
                     /gene="nlpA"
                     /locus_tag="ACHKW0_004613"
     CDS             3901..4719
                     /gene="nlpA"
                     /locus_tag="ACHKW0_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HFO7477294.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4723..5646)
                     /gene="yicL"
                     /locus_tag="ACHKW0_004614"
     CDS             complement(4723..5646)
                     /gene="yicL"
                     /locus_tag="ACHKW0_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate/amino acid/amine transporter"
                     /protein_id="HFO7477295.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5757..6941)
                     /gene="setC"
                     /locus_tag="ACHKW0_004615"
     CDS             complement(5757..6941)
                     /gene="setC"
                     /locus_tag="ACHKW0_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005351 - carbohydrate:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HFO7477296.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANSQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFWLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1788 a         1602 c         1786 g         1924 t
ORIGIN      
        1 aatatccatt aatgtgcaat tgaaatgtga taattatcac tgaaccgttg ttgtcatttg
       61 ttttacaaaa gcaagggatt aaatctcctc caatggacaa aaaaatgaat aatgacaata
      121 ccgattacgt gagtaatgaa tcagggacgc tttcgcgatt atttaaacta cctcagcatg
      181 ggaccaccgt ccgcacagaa ttgattgcgg ggatgaccac ttttttaacc atggtgtaca
      241 tcgtttttgt gaacccgcaa atcctcggcg cggcacaaat ggacccgaaa gtggtgtttg
      301 ttaccacctg tttgattgcc ggtatcggca gtattgcgat ggggatattt gctaacttac
      361 ccgtggcgct ggctccggca atggggctga acgccttctt tgccttcgtg gtcgtggggg
      421 cgatgggcat ctcctggcag accgggatgg gcgcaatatt ctggggcgca gttggactat
      481 ttttgctcac gctgtttcgt atccggtact ggatgatctc caacattccc ttaagtttac
      541 gtattggtat caccagcgga attggattat ttatcgcctt aatgggatta aaaaatactg
      601 gcgttattgt cgccaataaa gacacgctgg tgatgattgg cgatttaagt tctcacggcg
      661 tgttgttagg tattttaggg ttttttatta taaccgtgtt gtcatcacgt cattttcatg
      721 ccgcggtgct ggtttctatt gtggtgacgt cttgctgtgg attatttttc ggtgatgttc
      781 attttagcgg cgtctattcc attccgcctg atattagcgg cgtcattggt gaagtagatt
      841 tgagcggcgc gttaacactt gaactcgccg gtatcatttt ctcctttatg ctgatcaacc
      901 tatttgattc atcaggaaca ttaattggtg taactgataa agcgggctta atagatagta
      961 acggtaaatt ccccaatatg aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg
     1021 gggcgtttat cggcacctcg tctgttactg cctatattga aagtacttct ggtgtggcag
     1081 tcggtggtcg cacggggctg actgcggttg tggttggcgt tatgttcctg ttagttatgt
     1141 tcttctcacc gctggtggcg atggttcctc cttacgcaac cgccggagcg ttaatctttg
     1201 ttggcgtgct gatgacttcg agcctggcgc gcgttaactg ggatgatttt accgaatcgg
     1261 tgcctgcgtt tattaccacg gtgatgatgc cctttacttt ttcgatcacc gaagggattg
     1321 cactcggctt tatgtcgtac tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga
     1381 acctgtgtgt ggtggtggtc gcagctctgt ttgcactgaa gattattctg gtggattagt
     1441 aactggcgag ggtttccaga tatcatgagt tctgattacg caggagaact catgatctgg
     1501 ataatgctcg ccacgctggc ggtagtgttt gtggttggtt ttcgggtgct gacatccggg
     1561 gccagaaaag cgattcgccg tctcagcgat cggctgaata tcgatgtcgt acccgtggag
     1621 tcgatggtcg atcaaatggg aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc
     1681 ccggatgagt cgcacctgca aaacgccgcg caggtgttgc tcatctggca aattgtcatt
     1741 gtcgatggta gcgaacagaa cctgctgcaa tggcatcgga ttttacaaaa agcccgtctt
     1801 gccgcgccga ttaccgatgc tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa
     1861 cctgaaatgc aggatattaa tgcttttcag atgcgctata acgcgttctt tcagcctgcc
     1921 gagggcgttc actggctgca ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg
     1981 acctgtcggg tggataagac gttcacgccg catccggtaa tctttgccct gttccgtttc
     2041 gatcttaact ccagctcaag caacaccgta aaagttgcaa aaacgttaag tgcgtcacac
     2101 atttcaatgt taaagtggtc ggcttttccc ctgaaacatg ccacgggtaa caccatgagt
     2161 gaatttattg ccgaaaaccg cggcgcggat gccatcaccc gaccgaactg gtcagccgtt
     2221 ttctcggtgg cgttttgtgt cgcctgtctg attatcgttg agtttttgcc cgtcagtttg
     2281 ttgacgccaa tggcccagga tttaggcatt tcggaagggg ttgccgggca atcggtgacc
     2341 gtgaccgcct ttgtggcaat gtttgccagt ttgtttatta cccagacaat tcaggctact
     2401 gaccgccgct acgttgttat tttgtttgcc gttttgctga cgctctcctg cttgctggtt
     2461 tcctttgcta actcattcag tttgctttta atcggtcgtg cctgtctggg gctggcgctg
     2521 ggcgggttct gggcgatgtc ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg
     2581 ccgaaggcgc tgtcggtgat cttcggcgcg gtttctattg cgctggtgat tgccgcgccg
     2641 ttgggcagtt ttttaggcga gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg
     2701 atgggcgtgc tgtgtatttt ctggattatc aaatcattgc cttcactgcc aggcgaaccc
     2761 tcgcatcaga aacaaaatac tttccgctta ttacaacgtc cgggtgtgat ggcagggatg
     2821 atcgccatct tcatgtcttt cgccgggcag tttgctttct tcacatatat tcgcccggtg
     2881 tatatgaacc tggcgggatt cagcgtggat ggcttaacgc tggtgctgtt gagttttggc
     2941 attgccagct ttatcggtac gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg
     3001 gccttagcag gcgcgccgtt aatactggct gtgagtgcgt tggtactgac gttgtgggga
     3061 agcgataaaa tcgttgctac cggcgtggcg attatctggg ggctaacttt tgcattggtt
     3121 cccgtcggct ggtcaacgtg gatcacccgc tcgctggccg atcaggcaga aaaagccggg
     3181 tctattcagg tggcggttat tcagcttgct aatacctgtg gcgcggcaat cggcggttat
     3241 gcgctggata atattggtct gacttcgccg ttgatgttgt ccggcacatt gatgttgctg
     3301 actgcattgt tggttactgc aaaggtgaaa atgaagaaat cctgatatca ggtaaagaag
     3361 cgttggtcag tagttgttgc tgttcttaca tatccgggca ttctacctta cccaacgctt
     3421 cccaataatt gctctgatta ttacgttcca tcgccacgat ggcctcgcgt atatgttgtt
     3481 tattgttttc atctgacaac attaactttt cccacgcttc atctgataat gacgcctgtg
     3541 gcgtgcaaat tttacgtaaa tcttgtaata ccgtggtttt ctcttttgcg gactgcagtg
     3601 agctaaaagg cgactcggcg tatacgatgc ctggcatagc gaaaaaagtg aaaataagca
     3661 gtagtgtcgt tggtttcatg gattcaacct tttacaaatg tgtatcgaat gaagcataag
     3721 tgtagtttgc tttcatcgct ctcatcatgc tgtacgaaac gcgcttgcgt ggtcagtaag
     3781 aagtgccaga ctttatattc cacttttatt cctttttatt cttatcgata gcgtttcgtt
     3841 ttttaaaccg cagcgacctt accgctatag tcaggtaatc attaataaaa ggataaaaaa
     3901 atgaaactga caacacatca tctacggaca ggggccgcat tattgctggc cggaattctg
     3961 ctggcaggtt gcgaccagag tagcagcgat gcaaaacaca ttaaagttgg cgttataaat
     4021 ggcgcagaac aagatgtcgc ggaagtcgct aaaaaggtgg caaaagagaa atatggcctc
     4081 gatgttgaac tggtaggatt tagcggttcg ttgctaccga acgatgcgac aaatcatggc
     4141 gaacttgatg cgaatgtctt ccagcatcga ccctttcttg aacaagataa tcaggcgcat
     4201 ggttataaac tggtagcggt gggaaatacg tttgtcttcc ctatggcggg ttattccaaa
     4261 aaaatcaaaa ccgtggcgca aataaaagaa ggcgcgacag tggcgatccc caacgacccg
     4321 accaaccttg gtcgcgcact tttactgttg caaaaagaga agctaattac tctgaaagag
     4381 ggaaaagggt tattacctac cgcgctggat ataaccgata atcctcgtca tttgcagatt
     4441 atggaactcg agggggcgca gcttccgcgc gtactggatg atcccaaagt tgatgtagcg
     4501 attatcagta ccacttacat tcagcagaca gggctttctc cggtgcacga cagcgtattt
     4561 attgaagata agaattcgcc gtatgtgaat attttggtgg cacgggaaga taataagaat
     4621 gcagaaaacg tgaaggaatt tctgcaatct tatcaatcac ccgaagtcgc taaagcggca
     4681 gaaaccattt ttaacggtgg cgcggtgcct ggctggtaat tctcactcat gccgcgccgg
     4741 acgatttatt tttctggcgc ggcgacggga atccattgaa atcaaaatca ccgatgacag
     4801 aatcagcagc gttcccagcc agtccggtaa tgtgaacgtg atccccagca acagcaaaga
     4861 gagtagcgcg ctacttaatg gttctgcaca gctcaaaatg ctggcttttg gaccgccaat
     4921 taattgtgct cctttcaggt acagactaaa tgtcagggac gtaccaatga ccaccaaata
     4981 aaaaaacgcc agaatcaaac tgccgttaac gacaaagttt gttccttgtc tggcataaaa
     5041 aggcaacaga atcagaccgc caatcagcat actccagccg acgactggta acgtgccata
     5101 gcgggcaatt agcgttgagg gataggtggt atagaatgca gcagcaaatg ccgaggcaat
     5161 gccccagaac aacgcggcag gagagatcga taatgacgtc ggattaccgt gtgtcaccaa
     5221 taaaaaagtc ccgaccagcg atgtcaaaat agcgcagaaa accagaatgc ccgggcgcga
     5281 tttacgcacc agtgagaacc aggcgacgat aatcgtcggt gagaggaatt gcagcaccgt
     5341 tgccgtggct gcgttcgatt tttcgatggt tagcaaaaaa gtgagctgta cagttagcgc
     5401 gccaaccacg gaaaaaatca gcaggctaat ggcatcttta tgattgttaa taatagaaaa
     5461 gattttatcg ccatgaacaa atgacagcgt cagtagaatc aaaccggcga atatcaaacg
     5521 cgtcatagtc aaaaactgcg acgacatctg gctttgctcc atgatgtatt gcgcgcagac
     5581 ccctgaactt ccccacaaca cggcggcaat cagaacgttc agcatcccct ttctggtgga
     5641 acccattttt ccctctgcat actgttttgt cgtttatgta tgcaggcatg atagcaaaaa
     5701 tggcgaggat ggctctgttt gcgccacgcc gatttttctt agcccagact ggtgagctaa
     5761 atatctttaa taaacagcag gcaaatcatc gcaataccca acatccctat cgccagccag
     5821 aacaacgcat ggtagctcca gatttcaacc ataattccgt caacagagcc ggcgataatc
     5881 cagccgacgc gtgaagtatt tgcatataac gttgtcgcag agcctatttt ttcaggcatc
     5941 aagtcctgaa aataaagcat gccgatacca cagagaatac caaggaagat ggcattaaga
     6001 atttgcaatt ccagctcaac cgccggagtc gtcgccatga gtacgctggc gtaaaaacac
     6061 attccactca cgatagcaat gagcattaat agccgcttgc caatacgttt catgtaatag
     6121 cctgcgatta acatcatcgg aatttccaga ccggcagcga taccaatcat ttctccagcc
     6181 agtttatcgg ttagatgcag ttcatcaata ataaatagcg gcatatttat catgtagaga
     6241 ttattcgccg cccacatcat tgaacagacc acaaaaagta gccgcgtatc ccgcttcctg
     6301 tgggtggagg gtaaaatttc tacgggttgg gtaacgacag gaatatttct ttgtattgat
     6361 ggcaaaaaca accagactat cagtccgcaa acaacaaatg cgatggcagc ggtgagatac
     6421 atcactttaa aactaaatcc cattgccagt tcataagcaa gcggtggccc gataacccag
     6481 gcaagcgaga tctgagcacg taaaaatgta ctgaacataa ccgtctcacg gcctgttctg
     6541 tcggcgtgtt cacgggcgag ggcgaacatt tgcgagtttg cggtagaagc aaaactactc
     6601 agaagtacgc ccgttgagag cagaatgaag tagttgcgat tccacgcaaa aagcgtgcag
     6661 gccagcactc caaataagca acatagcaga atcagtaatt tacggtcgcc ttgtttatcg
     6721 gagtgccttg ccagaaattg actgaccaga attcccataa tagcgctacc ggtgaagaaa
     6781 aaacctacca ttataggacg ggctttcagt tcatctgcga ggaatatact tagggtagga
     6841 gtctgaagag cgcccgcaat acccgtcaga aaggcgacaa gtaaaaatgc cgcggcagtg
     6901 agatcaagta tttttgatgg agtggtagcc gttttttgca ttcttctgaa tcaccgggcg
     6961 aaaaaatata actgagttac aaataagtac cagtcgaatg agtattgtat taagaatttt
     7021 tgagtatcgc tgatacaacc agaaagcaat gctagcgcaa tattataggg ccagcaattg
     7081 agattattag caaaagcatg
//
LOCUS       DAZFXT010000194         6987 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.194, whole genome shotgun sequence.
ACCESSION   DAZFXT010000194 DAZFXT010000000
VERSION     DAZFXT010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.194"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            440..1429
                     /gene="araF"
                     /locus_tag="ACHKW0_004616"
     CDS             440..1429
                     /gene="araF"
                     /locus_tag="ACHKW0_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HFO7477297.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMEVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MEITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1499..3013
                     /gene="araG"
                     /locus_tag="ACHKW0_004617"
     CDS             1499..3013
                     /gene="araG"
                     /locus_tag="ACHKW0_004617"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP-binding protein
                     AraG"
                     /protein_id="HFO7477298.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3028..4014
                     /gene="araH"
                     /locus_tag="ACHKW0_004618"
     CDS             3028..4014
                     /gene="araH"
                     /locus_tag="ACHKW0_004618"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HFO7477299.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4181..4981
                     /gene="otsB"
                     /locus_tag="ACHKW0_004619"
     CDS             4181..4981
                     /gene="otsB"
                     /locus_tag="ACHKW0_004619"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HFO7477300.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4956..6380
                     /gene="otsA"
                     /locus_tag="ACHKW0_004620"
     CDS             4956..6380
                     /gene="otsA"
                     /locus_tag="ACHKW0_004620"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HFO7477301.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACHKW0_004621"
     CDS             complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="ACHKW0_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HFO7477302.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1776 a         1631 c         1813 g         1767 t
ORIGIN      
        1 taatgagcac agtgataatt aatggcgaaa tgaatgacgt gcattttcca catctttgag
       61 ttgcggttat tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtt
      121 aaaaagaaga catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg
      181 gattaatctg ctgtgcattc gacaatttgt ctgacaaatt ggctttccct tatgtctttt
      241 cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg
      301 agaattaatt tctcgctaaa actatgtcaa cacagtcact tatcttttag ttaaaaggta
      361 atgctttgtt ttccgattaa tttaacgaat gtcattcgtt tttgccctac acaaaacgac
      421 actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc
      481 agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggttttc tggtgaagca
      541 accggaagag ccgtggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt
      601 agggtttgag gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga
      661 cagcctggct gccagtggcg caaaaggttt cgttatttgt actccggacc ccaaactcgg
      721 ctctgccatc gtcgcgaaag cgcgtggcta cgatatggaa gtcattgccg tggatgacca
      781 gtttgttaac gccaaaggta agccaatgga taccgttccg ctggtgatga tggcggcgac
      841 taaaattggc gaacgtcagg gccaggaact gtataaagag atgcagaaac gtggctggga
      901 tgtcaaagaa agcgcggtga tggagattac cgccaacgaa ctggataccg cccgccgccg
      961 tactacggga tctatggatg cgctgaaagc ggccggattc ccggaaaaac aaatttatca
     1021 ggtacctacc aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt
     1081 tcaacatccg gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtgctggg
     1141 cggcgtacgc gcgacggaag gtcagggctt taaagcggcc gatatcatcg gcattggcat
     1201 taacggtgtg gatgcggtga gcgaactgtc taaagcacag gcaaccggct tctacggttc
     1261 cctgctgcca agcccggacg tacatggcta taaatccagc gaaatgcttt acaactgggt
     1321 agcaaaagac gttgaaccgc caaaatttac cgaagttacc gacgtggtac tgatcacgcg
     1381 tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa
     1441 aattcccctc tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat
     1501 gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa
     1561 ggcgctgacg gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga
     1621 aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac
     1681 gggttctgta gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa
     1741 cgcgggtgtg gcgattattt accaggaact acatctcgtg ccggaaatga ccgtcgcgga
     1801 aaacatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa
     1861 ttatgaggcg ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct
     1921 gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaggcgc tggcgcgtaa
     1981 cgctaaaatt atcgcctttg atgagccaac cagctccctt tctgcccgcg aaatcgacaa
     2041 tcttttccgc gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca
     2101 ccgtatggaa gaaatatttg ccctcagcga tgccatcact gtctttaaag atggacgtta
     2161 tgtcaaaacc tttaccgata tgcagcaggt tgaccacgac gcgctggtgc aggcgatggt
     2221 cgggcgcgac attggcgata tctacggctg gcaacctcgt agttatggcg aggagcgcct
     2281 gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag
     2341 tggtgaaatt gttgggctgt ttggtctggt aggggcgggg cgtagcgaat taatgaaagg
     2401 cttgtttggc gggacgcaaa tcaccgcagg tcaggtttat atcgaccaac agccgatcga
     2461 tattcgtaaa ccgagccacg ccattgccgc aggcatgatg ctctgcccgg aagatcgcaa
     2521 agcggaaggc attattcccg tgcactccgt tcgcgacaat atcaacatca gtgccagacg
     2581 taaacatgtg ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca
     2641 ccacattcgt tcgctcaaca tcaaaacgcc gggcgctgag caactgatca tgaatctctc
     2701 aggcggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat
     2761 tttgctggat gaacctacgc gcggcattga tgttggtgct aagcatgaaa tttacaacgt
     2821 gatttatgcg ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg acttacctga
     2881 agtcctcggc gttgccgacc ggattgtggt gatgcgggaa ggtgaaatcg ccggtgaatt
     2941 gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca
     3001 ggcagttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg gtctggcgca
     3061 cctaagtcgt cattcagctt cgggcgtatc tgggatcagt acggcatgct ggtggtgttt
     3121 gcggtgctct ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg
     3181 aaagggttgg gcctggcaat ttccatgtcg gggatggtgg cgtgtggcat gttgttctgc
     3241 cttgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc
     3301 acggcggtgg ttatcaacct gactgaaagc ctgtggattg gcgtggcagc ggggttgctg
     3361 cttggcattc tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct
     3421 ctgatcacaa cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac
     3481 ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc taactggttc
     3541 ggtctgcctg cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgttgctg
     3601 aataaaacca cctttggtcg taacaccctg gcgattggcg ggaacgaaga ggctgcgcgt
     3661 ctggcgggtg taccggttgt tcgcaccaaa attattatct ttgttctctc tggcctggta
     3721 tctgcgatag ccggaattat tctggcttca cgtatgacta gtgggcagcc aatgacgtcg
     3781 attggttatg agcttattgt tatctccgcc tgcgttttag gtggcgtttc tctgaaaggt
     3841 ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa ttttaggcac cgtggaaaac
     3901 gccatgaacc tgcttaatat ttctcctttc gcgcagtacg tggttcgcgg cttaatcctg
     3961 ctggcagcgg tgatcttcga ccgttacaag caaaaagcga aacgcactgt ctgatgcttt
     4021 tttccacaac aatttaacgt tttttcccac cacagccagc cgccacaacg gttggctgtt
     4081 cttcattgca aatggcgacc cccgtcacac tgtctatact tacatgtctg taaagcgcgt
     4141 tctgcgcaaa acaataagaa aagagaagga ggagagccgg gtgacagaac cgttaaccga
     4201 aacccctgaa ctatccgcga aatatgcctg gttttttgat cttgatggaa cgctggcgga
     4261 aatcaaaccg catcccgatc aggtcgtcgt gcctgacaat attctgcaag gactacagct
     4321 actggcaacc gcaagtgatg gtgcattggc attgatatca gggcgctcaa tggtggagct
     4381 tgacgcactg gcaaaacctt atcgcttccc gttagcgggc gtgcatgggg cggagcgccg
     4441 tgacatcaat ggtaaaacac atatcgttca tctgccggat gcgattgcgc gtgatattag
     4501 cgtgcaactg catacagtca tcgctcagta tcccggcgcg gagctggagg cgaaagggat
     4561 ggcttttgcg ctgcattatc gtcaggctcc gcagcatgaa gacgcattaa tgacattagc
     4621 gcaacgtatt actcagatct ggccacaaat ggcgttacag cagggaaagt gtgttgtcga
     4681 gatcaaaccg agaggtacca gtaaaggtga ggcaattgca gcttttatgc aggaagctcc
     4741 ctttatcggg cgaacgcccg tatttctggg cgatgattta accgatgaat ctggcttcgc
     4801 agtcgttaac cgactgggcg gaatgtcagt aaaaattggc acaggtgcaa ctcaggcatc
     4861 atggcgactg gcgggtgtgc cggatgtctg gagctggctt gaaatgataa ccaccgcatt
     4921 acaacaaaaa agagaaaata acaggagtga tgactatgag tcgtttagtc gtagtatcta
     4981 accggattgc accaccagac gagcacgccg ccagtgccgg tggccttgcc gttggcatac
     5041 tgggggcact gaaagccgca ggcggactgt ggtttggctg gagtggtgaa acagggaatg
     5101 aggatcagcc gctaaaaaag gtgaaaaaag gtaacattac gtgggcctct tttaacctca
     5161 gcgaacagga ccttgacgaa tactacaacc aattctccaa tgccgttctc tggcccgctt
     5221 ttcattatcg gctcgatctg gtgcaatttc agcgtcctgc ctgggacggc tatctacgcg
     5281 taaatgcgtt gctggcagat aaattactgc cgctgttgca agacgatgac attatctgga
     5341 tccacgatta tcacctgttg ccatttgcgc atgaattacg caaacgggga gtgaataatc
     5401 gcattggttt ctttctgcat attcctttcc cgacaccgga aatcttcaac gcgctgccga
     5461 catatgacac cttgcttgaa cagctttgtg attatgattt gctgggtttc cagacagaaa
     5521 acgatcgtct ggcgttcctg gattgtcttt ctaacctgac ccgcgtcacg acacgtagcg
     5581 caaaaagcca tacagcctgg ggcaaagcat ttcgaacaga agtctacccg atcggcattg
     5641 aaccgaaaga aatagccaaa caggctgccg ggccactgcc gccaaaactg gcgcaactta
     5701 aagcggaact gaaaaacgta caaaatatct tttctgtcga acggctggat tattccaaag
     5761 gtttgccaga gcgttttctc gcctatgaag cgttgctgga aaaatatccg cagcatcatg
     5821 gtaaaattcg ttatacccag attgcaccaa cgtcgcgtgg tgatgtgcaa gcctatcagg
     5881 atattcgtca tcagctcgaa aatgaagctg gacgaattaa tggtaaatac gggcaattag
     5941 gctggacgcc gctttattat ttgaatcagc attttgaccg taaattactg atgaaaatat
     6001 tccgctactc tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac ctggtagcaa
     6061 aagagtatgt tgctgctcag gacccagcca acccgggcgt tcttgttctt tcgcaatttg
     6121 cgggagcggc aaacgagtta acgtcggcgt taattgttaa cccctacgat cgtgacgaag
     6181 ttgcagctgc gctggatcgt gcattgacta tgtcgctggc ggaacgtatt tcccgtcatg
     6241 cagaaatgct ggacgttatc gtgaaaaacg atattaacca ctggcaggag tgcttcatta
     6301 gcgacctaaa gcagatagtt ccgcgaagcg cggaaagcca gcagcgcgat aaagttgcta
     6361 cctttccaaa gcttgcgtag gagcggttaa tctcccgtaa gtggaactaa cagcacatcg
     6421 acctcacttg aggcaataac tcttttcgcc gagcaggatg ctcgcgaaaa gaaactgtga
     6481 ttgtgattac cgcaaatcac caaatcgaaa tgatgtttgt gacatacttc cagaatatgt
     6541 tcgcttaact caccgtaggc aataaacgtc ttgtcaacgg gataccccgc atcctgaatt
     6601 aacttatcaa gaaagctttg cgtttcttct tgcatcacgc tgcgtaaatc ttcaagcatc
     6661 ggcgcggcta attgattgta catttccggg tcggaagcga gagtaattaa actgatgtgt
     6721 ccctttactg gcctggcgat agataccgct tttgccagca gttgctgact ttccggtgta
     6781 accgcaacag cgacaagaat attgctatag ctcataacct gttccttatt ctgtgaactt
     6841 caggtgacat taaagcttta ctcctaatgg agaaacgacg caatcccaac tcggtcaaac
     6901 aggtaaatat tagtctgatt attagtaaag acaattaatc aatcactccc ggtgatcata
     6961 taatttcaaa tgtaattata agaaaat
//
LOCUS       DAZFXT010000195         6913 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.195, whole genome shotgun sequence.
ACCESSION   DAZFXT010000195 DAZFXT010000000
VERSION     DAZFXT010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.195"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..3092
                     /gene="agn43"
                     /locus_tag="ACHKW0_004622"
                     /gene_synonym="flu"
                     /pseudo
     CDS             1..3092
                     /gene="agn43"
                     /locus_tag="ACHKW0_004622"
                     /gene_synonym="flu"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
     gene            3213..5729
                     /locus_tag="ACHKW0_004623"
     CDS             3213..5729
                     /locus_tag="ACHKW0_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477303.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            5805..6260
                     /locus_tag="ACHKW0_004624"
     CDS             5805..6260
                     /locus_tag="ACHKW0_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IrmA family protein"
                     /protein_id="HFO7477304.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            6339..6572
                     /locus_tag="ACHKW0_004625"
     CDS             6339..6572
                     /locus_tag="ACHKW0_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO7477305.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            6672..>6913
                     /locus_tag="ACHKW0_004626"
     CDS             6672..>6913
                     /locus_tag="ACHKW0_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7477306.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYT"
BASE COUNT         1674 a         1818 c         1951 g         1470 t
ORIGIN      
        1 aacaccagct acaggctggt atggaatcac attacgggca ccctggtggt ggcctccgaa
       61 ctggcccgct cacggggaaa acgcgccggt gtggcggttg cgctgtctct tgctgctgtc
      121 acatcagtcc cggcactggc tgctgacacg gttgtacagg cgggagaaac cgtgaacggc
      181 ggaacactgg caaatcatga caaccagatt gtcttcggta cgaccaacgg aatgaccatc
      241 agtaccgggc tggagtatgg gccagataac gaggccaata ccggcgggca atgggtacag
      301 gatggcggaa cagccaacaa aacgactgtc accagtggtg gtcttcagag agtgaacccc
      361 ggtggaagtg tctcagacac ggttatcagt gccggaggcg gacagagcct tcagggacgg
      421 gcagtgaaca ccacgctgaa tggtggcgaa cagtggatgc atgagggggc gatagccaca
      481 ggaaccgtca ttaatgataa gggctggcag gtcgtcaagc ccggtacagt ggcaacggat
      541 accgttgtta ataccggggc ggaaggggga ccggatgcag aaaacggtga taccgggcag
      601 tttgttcgcg gggatgccgt acgcacaacc atcaataaaa acggtcgcca gattgtgaga
      661 actgaaggaa cgacaaatac cactgtggtt tatgccggcg gcgaccagac tgtacatggt
      721 cacgcactgg ataccacgct gaatggggga taccagtatg tgcacaacgg cggtacagcg
      781 tctgacactg ttgtgaacag tgacggctgg cagattgtca aaaacggggg tgtggccggg
      841 aataccaccg ttaatcagaa gggcagactg caggtggacg ccggtggtac agccacgaat
      901 gtcaccctga agcagggcgg cgcactggtt accagtacgg ctgcaaccgt taccggcata
      961 aaccgccttg gagcattctc ggttgtggag ggtaaagctg ataatgtcgt actggaaaat
     1021 ggcggacgcc tggatgtgct gaccggacac acagccacta atacccgcgt ggatgatggc
     1081 ggaacgctgg atgtccgcaa cggtggtacc gccaccaccg tatccatggg aaatggcggt
     1141 gtactgctgg ccgattccgg tgccgctgtc agtggtaccc ggagcgacgg aaaggcattc
     1201 agtatcggag gcggtcaggc ggatgccctg atgctggaaa aaggcagttc attcacgctg
     1261 aacgccggtg atatggccac ggataccacg gtaaatggcg gactgttcac cgccaggggc
     1321 ggcacactgg cgggcaccac cacgctgaat aacggcgcca tacttaccct ttccgggaag
     1381 acggtgaaca acgataccct gaccatccgt gaaggcgatg cactcctgca gggaggctct
     1441 ctcaccggta acggcagcgt ggaaaaatca ggaagtggca cactcactgt cagcaacacc
     1501 acactcaccc agaaagccgt caacctgaat gaaggcacgc tgacgctgaa cgacagtacc
     1561 gtcaccacgg atgtcattgc tcagcgcggt acagccctga agctgaccgg cagcactgtg
     1621 ctgaacggtg ccattgaccc cacgaatgtc actctcgcct ctggtgccac ctggaatatt
     1681 cccgataacg ccacggtgca gtcggtggtg gatgacctca gccatgccgg acagattcat
     1741 ttcacctcca cccgcacagg gaagttcgta ccggcaaccc tgaaagtgaa aaacctgaac
     1801 ggacagaatg gcaccatcag cctgcgtgta cgcccggata tggcacagaa caatgctgac
     1861 agactggtca ttgacggtgg cagggcaacc ggaaaaacca tcctgaacct ggtgaacgcc
     1921 ggcaacagtg cgtcggggct ggcgaccagc ggtaagggta ttcaggtggt ggaagccatt
     1981 aacggtgcca ccacggagga aggggccttt gtccagggga ataagctgca ggccggtgcc
     2041 tttaactact ccctcaaccg ggacagtgat gagagctggt atctgcgcag tgaaaatgct
     2101 tatcgtgcag aagtccccct gtatgcctcc atgctgacac aggcaatgga ctatgaccgg
     2161 attctggcag gctcccgcag ccatcagacc ggtgtaaacg gtgaaaataa cagcgtccgt
     2221 ctcagcattc agggcggtca tctcggtcac gataacaacg gcggtattgc ccgtggagcc
     2281 acgccggaaa gcagcggcag ctatggcttc gtccgtctgg agggtgacct gctcagaaca
     2341 gaggttgccg gtatgtctgt gaccgcgggg gtatatggtg ctgctggcca ttcttccgtt
     2401 gatgttaagg atgatgacgg ctcccgtgcc ggcacggtcc gggatgatgc cggcagcctg
     2461 ggcggatacc tgaatctgat acacaacgcc tccggcctgt gggctgacat tgtggcccag
     2521 ggaacccgcc acagcatgaa agcctcatcg gacaataacg acttccgcgc ccggggctgg
     2581 ggctggctgg gttcgctgga aaccggtctg cccttcagta tcactgacaa tctgatgctg
     2641 gagccgcaac tgcagtacac ctggcaggga ctctccctgg atgacggcca ggataacgcc
     2701 ggttatgtga agttcgggca tggcagtgca caacatgtgc gtgccggctt ccgtctgggc
     2761 agccacaacg atatgaactt tggtaaaggc acctcatccc gtgacaccct gcgcggcagt
     2821 gccaaacaca gtgtgcgtga actgccggtg aactggtggg tacagccttc tgttatccgc
     2881 accttcagct cccgggggga catgagcatg ggtacagccg cagccggcag taacatgacg
     2941 ttctcaccgt cacagaatgg tacgtcactg gactggaagc ccgtgtccgg gaaaatatca
     3001 ccctgggcgt tcaggcaggt tatgtccaca gcgtcagcgg cagcagcgct gaaggctata
     3061 acggtcaggc cacgctgaat gtgaccttct gacagaacca tcgcctctct gtggtcccgg
     3121 tcatcatgat cgggacccgg accggcgcaa cggatattca acgccacatt cgctggcatt
     3181 aacaataaca tgatattcat cacggagtga ctatgttaca gatagtcggc gcgctgatcc
     3241 tgctgatcgc aggatttgcc attcttcgcc ttttgttcag agcattaatc agcacggctt
     3301 ctgcgctggc agggctcata ttgctgtgtc tgttcggccc ggccttactg gctggctata
     3361 tcaccgaacg cataacccgg ttgttccata ttcgctggct ggcaggcgta tttctgacga
     3421 ttgccggaat gatcatcagc ttcatgtggg gacttgatgg taaacatatc gcgctggagg
     3481 ctcacacctt tgactctgtg aaatttattc tgaccaccgc tctcgccggt ggtctgctgg
     3541 ctgttcccct gcagatcaaa aacattcagc agaacgggat cacaccagaa gatatcagca
     3601 aggaaattaa cgggtattac tgctgttttt atactgcctt tttccttatg gcgtgttctg
     3661 catgcgcacc attgatcgcg ttacagtacg atatttcacc gtcactgatg tggtggggcg
     3721 ggttgttgta ctggctggct gcattagtga cgctgctatg ggcggccagc caaatccagg
     3781 cgctgaaaaa actgacctgt gccatcagcc agacactgga agaacaaccg gtgctcaaca
     3841 gtaaatcgtg gctgaccagt ttgcaaaacg attacagcct tcctgactca ctgacggagc
     3901 gcatctggct gacgctcatt tctcaacgga tttcccgggg agagctgagg gaatttgaac
     3961 tggcagacgg aaactggtta ctgaacaatg cctggtatga aagaaacatg gcagggttta
     4021 acgaacagtt gaaagagaac ctgtcattca cacctgatga actgaaaacg ctcttccgga
     4081 accgcctgaa tttatcaccg gaagcgaatg acgattttct cgatcgttgc ctggacggcg
     4141 gtgactggta tcccttttca gaaggtcgcc gttttgtatc attccatcac gtggatgagc
     4201 ttcgtatctg tgcctcctgc gggctgacag aagtacatca tgccccggaa aatcataagc
     4261 cggatccgga atggtactgc tcctctcttt gtcgcgaaac agaaacactg tgtcaggaaa
     4321 tttatgaacg cccttacaac agctttattt ccgatgcaac ggcgaatggt ctgattctca
     4381 tgaaactgcc ggaaacctgg agtacaaatg agaaaatgtt tgcttccgga gggcagggac
     4441 atgggtttgc cgctgaacgg ggaaaccata ttgtcgacag agtccgtctg aaaaacgcac
     4501 ggatcctcgg tgataataat gccagaaatg gagcagacag actggtcagc ggaacagaaa
     4561 tccagacgaa atattgttca actgcagccc gtagcgtcgg tgcggcattc gacggacaaa
     4621 acggacagta tcgttacatg ggaaataatg gccccatgca actggaagtc ccccgtgatc
     4681 agtatgccgg cgctgttgaa accatgagga ataagatccg cgaaggtaaa gtacccggtg
     4741 taaccgatcc cgcagaagca tcccggctga ttcgtcgggg acatctgact tatactcagg
     4801 cccgtaatat cacccggttc gggaccatcg aatcggtcac ttatgatatt gccgagggtt
     4861 cggttgtcag tctggcggcc ggagggatca gttttgccct gacggcatcg gtcttctggc
     4921 tcagcaccgg cgatcgcgat gctgccctgc agacagctgc tgtccaggca ggaaaaacct
     4981 tcacccgcac actggctgtc tacgtcacaa cccagcaact ccaccggctc agtgttgttc
     5041 agggtatgct gaagcacatt gatttttcga ctgccagccc gactgtccga ctggctcttc
     5101 agaaggggac cggtgcagga aatatcagtg ccctgaacaa agtgatgaag ggtacgctgg
     5161 tgacatctct ggcactggta gctgtcacaa ccggccctga catgatcaaa atgttgcggg
     5221 gacggatctc cggtacgcag ttcatcagga atcttgccgt ggcatcttcc ggtgtggcag
     5281 gtggtgctgt cgggtcagtg gcgggcggaa tactgttcag tccactggga ccatttggtg
     5341 cactgacagg gcgtgtggtt ggcggtgttc tggggggaat gattgcctcc gctgtatcag
     5401 gaaaaattgc cggagcgctg gttgaagaag atcgcgtcaa aattctggca atgattcagg
     5461 agcaggtgac atggcttgcc ggcagtttcc tgctgaccgg acatgagatt gaaaatctga
     5521 acgagaatct ggcccgtgtt atcgatcaga atgctctgga gatcattttc gccgccggta
     5581 tacaacaacg ggccgcgacc aatatgttaa tcaaaccact ggtggtcagt atcatcaggc
     5641 aacgccccgt catggaatat gatgcatccc atctcggcaa tatggttaac cgactggaag
     5701 aagcattacc cccggagtta ccggcataac ggctattctg cgccttttac gctttatcac
     5761 ctgatttgtc agtgtcatcg tcatgactca gagaggtaaa taccatgatt cacctgttca
     5821 aaacctgcat gattaccgcc ttcattctgg ggttaacgtg gtctgcccca ctccgggcac
     5881 aggatcaacg ttacatcagt atacgcaata cagatacgat atggctcccg ggaaatattt
     5941 gtgcttacca gttccggctg gataatggcg gaaacgatga aggatttggc cccctcacca
     6001 tcactctgca actcaaagac aaatatggtc agacgctggt gaccagaaaa atggaaacgg
     6061 aagcttttgg tgacagtaat gccacgcgaa ccacagacgc atttctggaa acggagtgcg
     6121 tggaaaatgt cgccacaacc gaaatcatta aagcaactga agaaagtaac ggccatcgtg
     6181 tcagtctgcc gttatcggtt ttcaatcccc aggactacca tccactgctg attaccgttt
     6241 ccggaaaaaa cgttaactga tccctgaagt cagtttcatg cttcagcctc tgtcacaacg
     6301 cgtggcagag gctttttatt tatcaggaga aaacagatat gccgggttac acagagtatg
     6361 tcctggctga aggatctttc tcatacgggc aggcggttgc tgtcataaca gcttaccgca
     6421 atgtgtttat ccaggatgac ccgggaatgc atttccgtcg ggttatccgc aacgcggaag
     6481 gtcagcgacg ctggcgctgc cggaattctg aacccgatgc cggaaaggtg cttaatacgc
     6541 ggctcgccag tgacggtctt ctcagacaat aaacgtcatt catttcaccc atcaggccgc
     6601 gtcttctccg ggagacgtgg ctttttcatt tattacacca gtaaatctta accaccgata
     6661 aggagcaaat tatgcgacta gccagtcgtt ttggttatgc gaaccagata cgccgtgacc
     6721 gtccgctgac acacgaagaa ctgatgcact atgtgccggg gattttcggg gaagataaac
     6781 acacgtcccg aagccagaac tatacgtaca tccccaccat caccgtactg gaaagcctgc
     6841 agcgggaagg ctttcagcca ttcttcgcct gccagacccg tgtgcgcgac ccgggccgcc
     6901 ggggatacac aaa
//
LOCUS       DAZFXT010000196         6814 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.196, whole genome shotgun sequence.
ACCESSION   DAZFXT010000196 DAZFXT010000000
VERSION     DAZFXT010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.196"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(280..765)
                     /gene="sixA"
                     /locus_tag="ACHKW0_004627"
     CDS             complement(280..765)
                     /gene="sixA"
                     /locus_tag="ACHKW0_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0101006 - protein histidine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HFO7477307.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(968..3112)
                     /gene="fadJ"
                     /locus_tag="ACHKW0_004628"
     CDS             complement(968..3112)
                     /gene="fadJ"
                     /locus_tag="ACHKW0_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009062 - fatty acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HFO7477308.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3112..4422)
                     /gene="fadI"
                     /locus_tag="ACHKW0_004629"
     CDS             complement(3112..4422)
                     /gene="fadI"
                     /locus_tag="ACHKW0_004629"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003988 - acetyl-CoA C-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HFO7477309.1"
                     /translation="MGQVLPLVIRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4602..4886)
                     /locus_tag="ACHKW0_004630"
     CDS             complement(4602..4886)
                     /locus_tag="ACHKW0_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HFO7477310.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFASRAEAEQTL
                     AALTEKARSVESEPCKITPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5258..6598
                     /gene="fadL"
                     /locus_tag="ACHKW0_004631"
     CDS             5258..6598
                     /gene="fadL"
                     /locus_tag="ACHKW0_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HFO7477311.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1700 a         1831 c         1614 g         1669 t
ORIGIN      
        1 tcaagtttca attacatcct gcctgattat tcaactctat tttctctatt atctgtatta
       61 ataaatgaca atgagtgtca aacagacatt attcatttca acatgtaact ttgggcaaaa
      121 tcaagatacg cgcagaagaa taatttccga atgaatgtag aaagtgtttt atttatagat
      181 aaggagagtc ttcgatagta gtgaggttga cagatatccg ctgcctgatg agcaattatg
      241 cccaatgaat ctacctcatt gggcataatt tggaacacat cagatagctt ttgccatctt
      301 cagattacac gggctcatct gccagttgaa tgtaccgttt ccgctttcat caagcgttac
      361 gctggcaatg gccgaggtgg taaacatagg cggcgtttcg cccggacata actcggcaac
      421 cagatacccg actaacggta agtgagagat caccagtacc gaagcaacac cttcattggt
      481 caacgcctgc aaataggcac tgaccagacc aacatcgccg cagggcgtta actccggcag
      541 aacttctgca ctggaaggca ggttcagaca atcccctact tcttccagtg tttgctcggc
      601 tcgcaggaac gggctcacca gaacgcgttc gatttccact ttttgacctt tcagccagtt
      661 cgccatcagg cgagattcgt cacaaccatt agtggtcaga ggacgaacgg aatcactggc
      721 ggcatcgagg gctgcgtcgc cgtgacgcat gataaaaact tgcatattgc accgcttttg
      781 ttaaccagat tcgcccgttt tctttcatct gaaaccagag aaagaaaacg gtggccggca
      841 ttgtgcctta tccattcacc gaatgaaacg ctgtttttta cctcaatggc gtaagtatag
      901 tcaatatgcg tttacatttt gaccaacact ccgccattca gcgcggattc atatagcttt
      961 gaccttctta ttgcaggtca gttgcagttg ttttccaaaa actttcccca cgcgcgccca
     1021 tctcgaccaa acgctcgcaa ggggtaaaac gggaaccata ctgcgtggca agtcgttgca
     1081 ttattgcaac cacttcgccc gcgccgagag aatcgatata gcggaacggt ccaccgagaa
     1141 atggcggaaa accaatgcca aataccgcac caatatcccc gtcacgcacg ctacggataa
     1201 cctgctcatc aacacaacgt actgcttcat tcagcatcaa catcacacac cgttcagcaa
     1261 cctgcggtgc ggagagtcgc ccctgccctt gtgcgccaat gagcgggtaa atggcgggat
     1321 cgacctgttt tttgctttta cgccctttct gaccataaag atagaaaccc cgaccatttt
     1381 ttctgccttt gcgatcgtcg ttcaaaattg aagaaacaac atttgcaggc gcgctaaaac
     1441 gttctccata agcggcttcc agtacaggaa taattttagt cccggtgtcg attcctacct
     1501 catccaaaag ttggattgga cctaccggaa aaccaaattt cactagcgcg gcatcaatgt
     1561 gctctacccg ttcaccttcg gtcagcatgc ggatagcttc attaatgtaa ggcgctaaga
     1621 tgcgattgac gtaaaacccg gctttgtcac gcacgacaat gggcgttttg ccctgttttt
     1681 tcgccagttt taccgtagtg gcgatggttt gcgctgatgt acccgcatga ggaataatct
     1741 ccaccagcgg cattttttcc accggactga agaaatgcag gccgataact tgctcaggtc
     1801 gcgtggcgtg agcggcgata tcaccaatcg gtaaagatga cgtattcgaa gcaaagatgg
     1861 tatgagcggc gctattttgc tcaacttccg ccaccatctg ttgtttcaat tcgagatttt
     1921 caaacaccgc ttcaataatc agatcgcgat gggcaaagcc gcgatagtcc gtcgttccgg
     1981 agattaatgc cagctgtttg tcacgttcgc tggctttgag atgacgacgg cgaactttgc
     2041 cctccagctg atcccaactg tacttcagcg catgatttat gccctgcggg ttgatatctt
     2101 taattctgac cggaagcccc gctttacaag cagtgacata agcaataccg ccgcccatca
     2161 agccaccacc taaaattccc acgctgttta atggcgcagg cggtgcatca ctaccgggat
     2221 ctttcttcac gtccgtactg gcaaaaaaga tactacgcag cgcctgcgat tgtggcgtca
     2281 tcgccagttc gccaaacgcc cgagcttcgg cgtcataacc gctgctggtg ccctgcgcta
     2341 atcccgtttc aacaacctcc aggatgcgtt ctgtcgccgg ataattgcct tgagttttgt
     2401 gttctgtttt cttgccgacc attttgaaca gcagcgcacg acctaacggc cccgccagaa
     2461 tacgctcgcg tacaggtaga gggcgggaag atgggcgatc cttcttcgcc agctcaacag
     2521 cgacttccag caaaatggag tgcggaacaa cgtcatccac cagccccagc tttaatgcct
     2581 gtttcgcccg aagttgtttt ccggtgagga tcatctctaa tgctgtgctg acgcctatca
     2641 gacgcggtaa acgctgggtg ccgcctgaac cgggtaacaa tccaagttgt acttcaggca
     2701 aaccgagcac cgttttagga tcgtcagtac aaacgcgacc gtggcacgcc agcgccaact
     2761 ccagcccacc acccaggcaa gcgccatgaa tagccgcgat aaccggaatg ggcaaagcat
     2821 gaatctccgc catcaactgt tggccctgcc gcgccagtgc ttccgcttct tgcgccgttt
     2881 tgcagttgcc gatcatgttg atgtctgcgc cagcaatgaa gttgtccggt ttagcggaga
     2941 caaacaccac gcctcgcaac tctttgtttt cacggagttg cttaataatg gcgcgcacct
     3001 gcgaggcaaa ctccgccttc agggtattca ttttctcacc cggtacgtcg atggtgatga
     3061 tagcaatgtt gtccagacga acattaagag taaacactga tgccatttcc attattccgc
     3121 ctccagaacc attgccgcgc caagcccacc ggcagcacag gcggtaacta aaccaaatcc
     3181 accgccgcga cggcgaagtt catgcaatgt ctgggtaatc atccgcgcgc cggtcgccgc
     3241 gaagggatgc ccgtaagcaa tcgaaccgcc aagcacgtta aatttgctat cgtccacttc
     3301 gccagtggca tgtgcacgcc ccagtacttc acgagcaaaa cgttcactac ccagcaactg
     3361 aatattcgcc agtgtctgag ctgcaaaggc ttcgtgcata tcgatcaatg tcagatcgct
     3421 cattgtcaaa ccggcacgtt ccagcgccag cggtgttgac caggccggac cgagcaacat
     3481 gtcctgccag acatcaatcg cagtaaatgc gtagctgcgc agatacccca gcggcaccag
     3541 ccctaattct ttcgcccggg attcagtcat caggatcacc gccgctgcgc catcggtcag
     3601 cggcgtactg ttcgccgccg ttaccgttcc gtgtttgcga tcaaacgccg ggcgcagctt
     3661 tgcgtaatcg gcaagcgagg aattaccgcg aatattgttg tcttcgacaa gcggttgttt
     3721 ataaggaggg ataaaggcag tcatcacctc ttctttgagt ttaccgtctg accacgcctg
     3781 agcggcacgc tgatgcgaac ggtgcgctaa tgcatcttgc tgttctcggg tgatgccgta
     3841 ggttttcgcc atttgttcgg cggtgtcgcc catccgcaag ccagtagaat attctgctac
     3901 cgcaggcggt acgggcatta agtcgcgcaa acgcaggcga gagaagagtt tcagtcgctg
     3961 gctcatggtt cgagctttgt tgacatcaac cagcacgcgc gccagttttt tactgacgcc
     4021 aattggcaat acggaagagg aatctgcccc accggcaatc cccgctcgga tagttcccgc
     4081 catcaggctt tctgcgacgt ttgcaaccgc ctggaaactg gtagcgcaag cgcggctgac
     4141 gctgtaagca tcggtatgca cattcattcc ggtaccgaga acaatttcac gcgcaatgtt
     4201 gggggcttca ggcatttgta cgacctgacc aaagaccagc tgttcaatca cttcagcggg
     4261 gatctcgctg cgtgccagca gttcgcctac caccatcttc cctaaatcaa ccgcgggaat
     4321 gccatgaaaa gccgtcgcct ggcgggcaaa aggcgtacgt aaaccgctaa caatggcgat
     4381 acgatcgccc tggcggataa ccagcggtaa aacctgaccc ataacactcc cctgtaaaaa
     4441 aataaataaa gtggtctgac ctgatcatag tcttaaccat ttttttacat ttagccaagc
     4501 ggagaaaaaa gaaagtggga gctatgacac agtgaagaca ggagaaagaa aagaaaacgc
     4561 ccctgccagc aactgacagg ggcgaacgta cccgagagga attaacgcag acccagctgg
     4621 aagatcagca tttcagcttc gcaggcaaaa gtgaaatcga tatccaggcg cacaccgtca
     4681 gactcttcag tgaaagtcgg ggtgattttg cacggttcgg attccacgct acgggctttt
     4741 tcagtcagcg ccgccagcgt ttgttctgct tctgcgcggc ttgcaaacac gcggctgtaa
     4801 gacgcggtgc agtcggagtt gtccataatg gtgccaacat ccatacagca gcaaaccggg
     4861 gtttcatcag cactacattt actcatcgtt gatttcctct gtatgtgcac ccaaggtgcc
     4921 agataaacgt tgtggatatt ttacgcttcc ggaaagtgct gctccagttg ttaattctgc
     4981 aaaatcggat aagtgaccga aatcacactt aaaaatgatc taaaacaaaa ttcacccgaa
     5041 tccgcgagtg cgcaacctcc aaattttgcc agctggatcg cgtttcttag atcatatttg
     5101 aaaaaagatg gaaacatact tgcaacattc cagctggtcc gacctatact ctcgccactg
     5161 gtctgatttc taagatgtac ctcagaccct acacttcgcg ctcctgttac agcacgtaac
     5221 atagtttgta taaaaataaa tcattgaggt tatggtcatg agccagaaaa ccctgtttac
     5281 aaagtctgct ctcgcagtcg cagtggcact tatctccacc caggcctggt cggcaggctt
     5341 tcagttaaac gaattttctt cctctggcct gggccgggct tattcagggg aaggcgcaat
     5401 tgccgatgat gcaggtaacg ttagccgtaa ccccgcattg attaccatgt ttgaccgccc
     5461 gacattttct gcgggtgcgg tttatattga cccggatgtg aatatcagcg gaacgtctcc
     5521 gtctggtcgt agcctgaaag ccgataacat cgcgcctacg gcatgggttc cgaacatgca
     5581 ctttgttgca ccgattaacg accaatttgg ttggggcgct tctattacct ctaactatgg
     5641 tctggcaaca gagtttaatg atacttatgc aggcggctct gtcgggggta caactgacct
     5701 tgaaaccatg aacctgaact taagcggtgc gtatcgctta aataatgcat ggagctttgg
     5761 tcttggtttc aacgccgtct acgctcgcgc gaaaattgaa cgtttcgcag gcgatctggg
     5821 gcagttggtt gctggtcaga ttatgcaatc tcctgccggg caaactcctc aagggcaagc
     5881 attggcagct accgccaacg gtatcgacag taataccaaa atcgctcatc tgaacggtaa
     5941 ccagtggggc tttggctgga acgccggaat cctgtatgaa ctggataaaa ataaccgcta
     6001 tgcactgacc taccgttctg aagtgaaaat tgacttcaaa ggtaactaca gcagcgatct
     6061 taatcgtgcg tttaataact acggtttgcc aattcctacc gcgacaggtg gcgcaacgca
     6121 atcgggttat ctgacgctga acctgcctga aatgtgggaa gtgtcaggtt ataaccgtgt
     6181 tgatccacag tgggcgattc actatagcct ggcttacacc agctggagtc agttccagca
     6241 gctgaaagcg acctcaacca gtggcgacac gctgttccag aaacatgaag gctttaaaga
     6301 tgcttaccgc atcgcgttgg gtaccactta ttactacgat gataactgga ccttccgtac
     6361 cggtatcgcc tttgatgaca gcccagttcc ggctcagaat cgttctatct ccattccgga
     6421 ccaggaccgt ttctggctga gtgcaggtac gacttacgca tttaataaag atgcttcagt
     6481 cgacgttggt gtttcttata tgcacggtca gagcgtgaaa attaacgaag gcccatacca
     6541 gttcgagtct gaaggtaaag cctggctgtt cggtactaac tttaactacg cgttctgata
     6601 acgcgttcgc ctggataaag tcacctgcat agcaggtgac tttaactccc ccacttcacc
     6661 gaaagtagtg tcctcattgc ttacctcctg agttttgcaa acagcctgtt ggcagcttgc
     6721 cctttcaaat cagtaagtgg tattcacaga caaacctgaa gggaaatgca tattttcaag
     6781 cgttctgctc gtccttctca aagagtttac tttt
//
LOCUS       DAZFXT010000197         6798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.197, whole genome shotgun sequence.
ACCESSION   DAZFXT010000197 DAZFXT010000000
VERSION     DAZFXT010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.197"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(193..1122)
                     /gene="fdhE"
                     /locus_tag="ACHKW0_004632"
     CDS             complement(193..1122)
                     /gene="fdhE"
                     /locus_tag="ACHKW0_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HFO7477312.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(1119..1754)
                     /gene="fdoI"
                     /locus_tag="ACHKW0_004633"
     CDS             complement(1119..1754)
                     /gene="fdoI"
                     /locus_tag="ACHKW0_004633"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HFO7477313.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(1751..2653)
                     /gene="fdoH"
                     /locus_tag="ACHKW0_004634"
     CDS             complement(1751..2653)
                     /gene="fdoH"
                     /locus_tag="ACHKW0_004634"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HFO7477314.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(2666..5716)
                     /gene="fdnG"
                     /locus_tag="ACHKW0_004635"
     CDS             complement(2666..5716)
                     /gene="fdnG"
                     /locus_tag="ACHKW0_004635"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(5129..5131),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HFO7477315.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            5910..6743
                     /gene="fdhD"
                     /locus_tag="ACHKW0_004636"
     CDS             5910..6743
                     /gene="fdhD"
                     /locus_tag="ACHKW0_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HFO7477316.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1483 a         1844 c         1845 g         1626 t
ORIGIN      
        1 agcgcatcag gcaatcttac gcttgtcatc agtgacatac caataacctt atcgatgtgt
       61 tggagggaag aacgaggcaa acaggcactg gcggcatagc ttgaggtggt aacgcggaat
      121 acgggagatg gtgtcgtcac accctatggg ctgtaatccg ggcgacctgc gccacccgga
      181 aaacgtcaga gattactccc cttcacccgg gaacaggaac gggttgatgg aactgcgggc
      241 gtagccttct tgctccattc gcgcgtccag taccagagag gcgaggtcat ctgccacggc
      301 ttcaactttc ggttcttttt cctgataaag aatcttcagg taagtgccgc agtcatcgca
      361 gctttcggct ttaatcgcgg cctgttcgtc atccagcgac cagtaatgca gtttgccgct
      421 ctgttcacag ttgctgcatt ttacgcgcac tacgtgccat tcggtttcac acaggttgca
      481 gtgcaggtaa cgcagaccct gagtggtgcc aatttgcacc atgctggaca ccggcataga
      541 accacaaacc gggcaatatt gacgttgctc gccgtattca gcgcgggctt tgccggggat
      601 cagattggcc atctgcgccc agtagagcga cagtgcagcc cagataaacg gcgctttatc
      661 gctgctgacg gatgagaaat cagaggcaaa cagtgcgctg gccatatctt ccagctcctg
      721 agtcgatgcc ttctccagat tctcaatcac tgccagcgcc gggccgctca tttcaggttt
      781 cagctcagca atcagcgcca tcagcagctt ttgccagtgc ttatcacgcg gcagaacgtg
      841 aatatccagc gggggcttgc cctgtgcgct ggcttcttta atgcgcgcgg tcagatccat
      901 ctccagcgga tggtcgtaca gcaccacttc ctgggcgtgg gcgataagcg cagcaaagcg
      961 caggtaatca cccagcggat tattttctgc cagctcgcgc agacgctcgg cgcggcggtt
     1021 gtataaattc ttgagccgag ggaacaataa cggcggaatc atatccgccg tacgtttctc
     1081 gctcgaaccc agctcatctt gcgggattat gcgaatactc attcagcttt cttttccgtt
     1141 gtcttgcgga cctcacggta ccagcgcggg tgatgtttct tcgcccaggc gctggtaacc
     1201 catccttcca ccatggcggt aatcgtgcct ttcacccaaa gggcggcgta gatatgcacc
     1261 atgataacca caattaacgc cactgcggca aatgaatgca gcattaacgc gaatcggatc
     1321 accgggattg agaaagcagg cgcaaaataa ggacgccaga taatcacgcc gctcaccagc
     1381 agcagaacca ggaaaataat cgccgcccag aaaacgcatt tctgaccgaa gttataacgc
     1441 ccggtgtcac ctacttcctc gttgacgacg atcttacgaa tattcttcgc ccaaaagata
     1501 tcatcccgat tgattaggtt gtgatgccag taacggaaaa acatgatgat gaacgaggca
     1561 aacataacca cgccgacaaa cgggtgcaga attcgcgcca gctgcggtgt gcccatgatt
     1621 tgcatcaacc agttgaagga cgggaacaaa aagcccagcc cgctcaccgc cgccaggatg
     1681 aagcagaagg cggtgatcca gtggttgata cgttccggcg cggtgtagcg cacgatggtg
     1741 tcacgtcgtt tcatttgcgc tcctcgtctt tctcttcgtg cagattattc tcttcctcat
     1801 ccgcacggtt cggaccgaca cccacgtagt ggaagatact ggccgcaaag gtagccgcaa
     1861 agccaacagc cgcgagcggt ttccagatgc ctttccagaa tttcacggtt tcgctgattt
     1921 ccgggttctc cggcaagcca tgatacagat ttggcttgtc agcatggtgc agcacgtaca
     1981 tgacgtgtgt accaccgacg cctgccggat cgtacagacc cgcattgtcg taaccgcggg
     2041 ttttcagctc agcaacgcgc tcgctcgcca gcgttttcat cgactctttc gtaccgaagt
     2101 gaatcgcgcc cgttgggcag gtcttcacgc aggctggttc ttgcccaacc accacgcggt
     2161 caacgcacag cgtacatttg tagacgcggt tgtcttccgg gttgaggcgc ggaatgtcga
     2221 acggacagcc cgcaatgcaa taaccgcagc caatgcactg ctcggactgg aagtcgacga
     2281 taccgttggc atactgaata attgcacctt ccgccgggca cgctttcagg cagcctggat
     2341 cggaacagtg catacagccg tctttgcgga tcagccattc cagtttgtcg ttctgctcca
     2401 cttccgagaa gcgcattacc gtccacgatt tggcgcttaa atcattgggg ttgtcgtaca
     2461 ccccaatgtt attgccgacg gtatcgcgaa tgtcgttcca ctctgaacac gccacctgac
     2521 aggctttaca gccgatacag gtggtgacgt cgatgagttt cgccacttct tcctggaagt
     2581 cccgcgcctg aggcgcgggg gtcagaccgt tagtcgcgga acgacgaatg atatcttgag
     2641 attgataagc catatgtcgt ctccgttaca ccttttccac attcacaagg aaggacttaa
     2701 actccggcgt ctgcgtgttc gcatcaccaa cgaatggcgt taacgtattg gcaataaagc
     2761 cttttttagc tacgccttca taaccccagt gaatcggaat accgatggta tcgatatctt
     2821 tgccgttcgc tttcagcgta cgaatacgtt tggtcaccac cgctttggct ttgatatagc
     2881 cacggttgga ggagactttc acggtatcgc cctgggcaat gccaagttta ttcgccagcg
     2941 actccccgat ttccacaaac tgctctggtt gcaaaatcgc gttcaacagc gcgtgtttgg
     3001 tccagtagtg gaagtgctcg gtcagacgat aggtggttcc gacatacggg aacttatcgg
     3061 ctttacccaa tgcttcggcg tcgtctttaa agatacgtgc agccgggttc gagataacgt
     3121 ttggatgcag cgggttagtt cccagcggcg tttcaaacgg ctcgtagtgt tccgggaacg
     3181 gaccttccgc catcttatcg agggcaaaca gacgccccat gccttcctgc tgcatgataa
     3241 acggcccgac gccgctaccc ggaggcgctg cgctgtagtc cggaatatcc cagccggtcc
     3301 acttagtgcc gtcccatttc agcaactgac gcttcggatc ccacgggtta ccctgcggat
     3361 ctgcggaggc gcggttatac agaatgcggc ggttaagcgg ccatgcccat gcccagccca
     3421 gcgtgttacc gaggccagac ggatcggcgt tatcacgacg cgccatctgg ttgccttccg
     3481 gcgtccagct accggcgaaa atccagcagc cacaggaggt tgtaccgtca tcgcgcaatt
     3541 gggcgaacga gctaagttgt tggcctttct tgacgataac cgcaccggtt gccggatcgg
     3601 taatatcggc cagcgcctta ccgttgctct ccatcgctac ttcttccgac ttcggctcat
     3661 gcggaatggc gtagttccag gtcatgttca gcacctggtc cgggttcgcg ccaccctgtt
     3721 cggcatacat cttgcgcaag cgcaggaaga taccggagag gatctcgcca tcagtcagcg
     3781 caatccccgg ggcgtccgca cctttccagt gccactgcaa ccagcgacct gagttaacga
     3841 ttgaaccgtt ctcttctgcg aagcaggtcg atggcagacg gaacacttcg gtctggatct
     3901 tcgacgagtc aacttcgttc agctcaccgt ggttctgcca gaagttagag gtttcagtgt
     3961 tcagtgggtc gatggtgacg aggaacttca gtttcgacag acaaccgatc actttgtttt
     4021 tgttcgggaa tgaggcaaca gggttaaagc cctggcagat atagccattg accttgccct
     4081 ctttcatcat ctcgaagtat tgcaggacgt cgtagccttt atcccacttc ggcaaccagt
     4141 caaagcccca gctattttcc gccgtcgctt tatcaccaaa gaaggccttc atcatagaga
     4201 cgaagaattt cgggtagttg ccccagtagt ttacctggcc ttccagcagc ggttttggcg
     4261 tgttggcggt aaggtaggtt tgcagatcgg tctgcttctc gcttggcagc gtcatgtaac
     4321 ctggcaggct ctgcgacagc agccccaggt ccgtcagccc ctgaatattg gagtgaccgc
     4381 gcagggcgtt aacgccgccg ccggccatcc ccatattgcc gagcagcagc tggatcatcg
     4441 ccatcgtacg aatgttttgc gcaccaacgg agtgttgcgt ccagccgagg gcatacagga
     4501 acgaggcagt tttatcgtga gcactggttt ctgcgatgta ttcgcagact ttcaggaacg
     4561 cgtcttttgg cgtaccacag atgttttcaa ccacatctgg cgtgtagcgg gaaacgtgct
     4621 gtttcagcaa gttccacacg cagcgcggat gttgcagcgt ggtatcgcgt ttggcgaagc
     4681 cgttttcgtc cagttcataa gtccaggagg atttatcgta cttgcgtttt tccgcgtcgt
     4741 agccggtgaa caggccatct tcaaagccgt aatcctcacg cactatcagg ctggcgttgg
     4801 tataggcttc ggtgtattcg cggttgaatt tttcattgtt cagcaggtac agcaatacgc
     4861 ctgacaggaa agcaatgtca gtaccggaac gaataggggc atagtagtca gctaccgccg
     4921 ccgtacgcgt aaagcgagga tcgatcacaa tcagcttcgc gccgttgtga attttggctt
     4981 ccatcgccca gcggaacccg accgggtgag cttcagcggc gttaccgccc atcaccacga
     5041 cgaggttggc gttcttgatg tcgacccagt ggttggtcat cgcaccgcga ccaaatgttg
     5101 gagcaagact tgctaccgtt ggtccgtgtc agacacgcgc ctggttgtcg accgcgagca
     5161 tacccagcgc gcgggagaat ttttgcgtta aatagccggt ttcgttactc gacgcggaag
     5221 cacacagcat cccggtggag agccagcggt taacagtcac gccttcggcg ttttgcgcaa
     5281 tgtagttagc atcgcggtct tctttcatca gtttggcgat gcgatcaaac gcctcttccc
     5341 aactgatttg ctgccattta tcagaacctg gcgcacggta ttccggaaac ttcagacggc
     5401 tttcggagtg gatgaaatcc accaggccag cgcctttcgg acaaagtgca ccgcggttga
     5461 ccgggtgatc cggatcgcct tcgatatgga agatagatgc tttggcgttt tttgctccgt
     5521 caccgaggct gtacatcaac agcccacagc ctacggaaca ataggtgcag gtattacggg
     5581 tttcgcgggt gcgcagcagt ttatactgcc gggtttccgc gagcgctacg ctgggtgcaa
     5641 aacccagtgc cgctgccgtg gtgcctgcca taccgccagc gcagatctta aagaactgcc
     5701 ttctgctgac ctgcatggat tgctccttgt ttcgacattg tcacgtccca tttacattcg
     5761 cttgctgcgt gtgcagggag tgggagttat ttttctttgc ggaaggggcc gcaaaggtcc
     5821 agaattggct caatttccct ccatccagga agggtttgta acagaatacc ataatgttgg
     5881 tgtgtgtgtt cttatctggt taagagaaag tgaaaaaaac acagcaaaaa gaaatcgaaa
     5941 atgtgacaaa tatcacaggt gttcgtcaaa ttgagttatg gcggcgtgat gatttacaac
     6001 acccacggtt agatgaggtc gcggaagaag ttcccgttgc gctggtctac aacggcattt
     6061 cgcatgtggt gatgatggcg tcgcccaaag accttgagta ctttgcgctc ggtttttcgc
     6121 tttccgaggg gataatagaa agtccgcgcg atatcttcgg catggatgtc gttccttcct
     6181 gtaatggcct tgaagtacaa attgagcttt ccagccgccg ctttatgggg ttgaaggagc
     6241 gccgccgggc gctggcggga cgtacgggct gtggcgtatg cggcgtggag caacttaatg
     6301 acatcggaaa accggtgcag ccgctaccgt tcacccagac gtttgatctc aacaaactgg
     6361 atgatgcatt acgtcatctc aacgattttc agccagtggg gcaactgact ggttgtactc
     6421 acgccgctgc ctggatgttg ccatctggcg aactggtcgg cgggcatgaa gacgtgggtc
     6481 gccatgtggc gctggacaaa ctgttaggcc gccggtcaca agaaggggaa agctggcagc
     6541 aaggtgcggt actggtttcc agtcgtgcca gttatgaaat ggtgcaaaag tcggcgatgt
     6601 gcggcgtaga gattttgttt gcggtgtctg ccgcgaccac gcttgctgta gaagtggctg
     6661 agcgctgtaa tctgacactg gtaggttttt gtaaaccggg tcgggcaacg gtttataccc
     6721 atccgcagcg tttgagcaat taattaaaat aagtcctgcg atatatatta aattgcagga
     6781 cttattcatt tcgtgaat
//
LOCUS       DAZFXT010000198         6750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.198, whole genome shotgun sequence.
ACCESSION   DAZFXT010000198 DAZFXT010000000
VERSION     DAZFXT010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.198"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..6167
                     /gene="efa1"
                     /locus_tag="ACHKW0_004637"
     CDS             <1..6167
                     /gene="efa1"
                     /locus_tag="ACHKW0_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lymphostatin Efa1/LifA"
                     /protein_id="HFO7477317.1"
                     /translation="FSDINTLSRYTLHGLGITGVHTEENLLPAPSSSLINILKEHYNE
                     DEISAKLPLAYDYILNKKESSSIPVEILNKLSELPPHELLTPVLGQSVNPLGMGYSSD
                     NGKITEQVIVSGADGFDNPISGLIYTYLEDLYNIHVRMREGTLNSQNLRQLLENSVSS
                     CFLTEQSINKLLSEAEKRPYQSLTEIHQHLTGLPTIADATLSLLSVGLPGTGKLLRRE
                     QDYGRPPVTAIQDSTFVLPYNFKGIGFNDNIISSAPVASSLHFIAEHAKYTLLSWPEF
                     YRHHAQRWFEMAKGYGSQNIDFHPQSLLVTQEGRCMGLALLYLQTKDTAHYSILQENL
                     MTVSALHQTSNRDKLPLSKDDNSLMTRTYSLIEMLQYQGNKYITNESLLHKTAWNQER
                     ITLLFNEKGVKRALISTPNHTLVLQQLEDIYRLTDPNFGHADFLSPIDALKFIEAMIQ
                     LTPTLQEYYGLLNKDINKHIQVHYAESDMVWNKLLPENDAGLSTRIQHTTTDRLANLA
                     EPVAVAGISLPVKTLYDIGATLDGRRITSPPTSEQIPSLRLNGDVLNDYLSRTVLTPE
                     QADNIRKILHTQGIRSGTRPIDPEMIRGTQDDLVSSQTRLQRQATRVKQQLAGVLDTL
                     QQHFQNIPRSSGRHLSVENIELADIGSGRFNLQIRDGETLHTTSVEVPEVVSRFQKLS
                     TMLSALPASGIMDFDLGMSVVGVVQYARLLQQGHEDSTLAKINLAMDIKQLSEATLGS
                     MIQIAGNKFLNTEGIQGFRLESAVAEGMRSVATRTGGTMGKALSASARVLELPVLETV
                     LGTWNLYNSVIQLQQATSYSETMAARVQIAFDSISLGLTAASVAFPPLIIATGPIAAI
                     GMGASSIARNVARKEERHTQWLEYKKFLTDGSKHIVVASPERGLLDFSGNKVFGKMVL
                     DLRQSPPLLHGESSFNADRKIGHRPDLGDWQIREKVGYANSISPYSSLAHGYANSKWP
                     RTIPKIPSGEYDTIILGYGHQYQANTEIEYLSNWIVWREAVPDSTSRHKRPPLEVLNS
                     QCTVIAGERKTTVLPLRVLSDLTPECTEQAISLKDYKFILRGGSGGLAVQVGGAGYYD
                     IDANPVAKENTLSFRGLPEEFPLTFDLSKQTQSVMLKTPDDEVPVMTITQKGINTLVG
                     TAAGKDRLIGNDKDNTFHTSSGGGTVISGGGNNRYIIPRDLKTPLTLTLSSNSVSHEI
                     FLPETTLAELKPVAFELSLIYWAGNNINVQPEDEAKLNHFAGNFRVHTRDGMTLEAVS
                     RENGIQLAISLCDVQRWQAVYPEENNRPDAILDRLHDMGWSLTPEVRFQGGETQVSYD
                     PLTRQLVYQLQARYSEFQLAGSRHHTTAVTGTPGSRYIIMKPVTTQILPTQIILAGDN
                     DHPETIDLLEASPVLVEGKKDKNSVILTIATIQYSLQLTISGIEESLPETTRVAIQPQ
                     DTRLLGDVLRILPDNGNWVGIFRSGHTPTVNRLENLMALNQVMTFLPRVSGSAEQVLC
                     LENLGGVRKKVEGELLSGKLKGAWKAEGEPTVPVNISDLSIPPYSRLYLIFEGKNNVL
                     LRSKVHAAPLKITSAGEMQLSERQWQQQEHIIVKPDNEAPSLILSEFRRFTISSDKTF
                     SLKLMCHQGMVRIDRRSLSVRLFYLREQPGIGSLRLTFRDFFTEVMDTTDREILEKEL
                     RPILIGDTHRFINAAYKNHLNIQLGDGVLNLADIVAEYARIQKEETSKILYQYQGAMK
                     KKTDGPSVVEDAIMTTTVTTDSGELFPTFHPWYTDDLSGRYKSVPMARKADTLYHLTP
                     KGDLQIIYQVATKMVNQAMIVSLPNYRHEWEKYNLSILSEIPQNNNTVVHSILRVNGP
                     TMQVRTIDYRGTDENNPIVSFSDTTFINGEQMLSYDSHSSGRVYSREEYMMWELQQRV
                     SEASSARTQDYWLMDAAVRNGEWKITPELLRHTPGYIRSTVSKWSRGWLKTGTILQTP
                     EDRNTDVYLTTIQNNVFSRQGGGYQVYYRIDGMAGADIADNAPGETRCTLRPGTCFEV
                     TSVDERHYEWNIIYVTLKTCGWSRNGQSKTPNGDNLFN"
     gene            6339..6689
                     /locus_tag="ACHKW0_004638"
                     /pseudo
     CDS             6339..6689
                     /locus_tag="ACHKW0_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         2016 a         1531 c         1527 g         1676 t
ORIGIN      
        1 tattttctga tatcaatacc ttgtctcgtt atacactaca cggactagga ataactggtg
       61 tacatactga agaaaacctg ctaccagctc cttcatcgtc cttaattaat atattgaaag
      121 aacattataa tgaagatgaa attagtgcga aattaccact agcatatgat tacattttaa
      181 ataaaaaaga atcaagctct attcctgttg aaattttgaa caaactttca gagttaccac
      241 cacatgaact actcacacct gttcttggcc agagtgttaa tcctctgggc atgggctact
      301 catccgataa tggaaaaatc acagagcaag taatagtcag tggagctgat ggatttgata
      361 atcccatatc tggacttata tatacctatc ttgaagatct atataacatc catgtaagga
      421 tgcgagaagg tacactaaat tcacagaatc ttcgtcagct tctggaaaac tctgtttctt
      481 catgcttttt gactgagcaa agtattaata aattacttag tgaggcagaa aaaaggcctt
      541 atcagtcttt aacagaaata catcagcatc tcacaggatt accaactatt gccgatgcaa
      601 ccctttcatt actttctgtt ggattacctg gtacgggaaa actattgcgc agggagcagg
      661 actatgggcg tccaccagtt acagcaattc aggattccac atttgtactc ccttataatt
      721 tcaaaggtat tggttttaac gataacatta tatcctctgc acctgtagcc tcctcattac
      781 attttatcgc tgaacatgcg aaatatactt tattgtcatg gcctgagttt tatcgtcatc
      841 atgcacagcg atggttcgaa atggctaaag gatatggaag ccagaatatt gattttcacc
      901 ctcagtctct attggtaacc caagaaggac gctgtatggg attagcctta ctttatttac
      961 agactaaaga tactgctcat tatagcattc tccaggaaaa cctaatgact gtgagtgcac
     1021 ttcatcagac cagtaatcgc gataagttgc cactgtccaa agatgataat tccttaatga
     1081 caagaactta tagtctgatt gaaatgctac agtatcaggg aaacaaatat attaccaacg
     1141 aatcgctact acataagacc gcatggaacc aagaaagaat aactttatta ttcaatgaaa
     1201 aaggagttaa gcgagccctg ataagcacgc ctaatcatac tctggttctg caacaactgg
     1261 aggatattta ccggctcact gatccaaatt ttgggcatgc agatttcctt tcacctatag
     1321 atgctctgaa gtttattgag gctatgatac aattaactcc aacacttcag gaatattatg
     1381 gcctattaaa caaagacatt aataaacata tacaagtaca ttatgccgaa tcagatatgg
     1441 tctggaataa gcttctgcca gaaaatgatg ctggactgag caccagaatt cagcacacca
     1501 ccaccgaccg tctggcgaat ctggctgaac cagtcgctgt tgcaggtatc tccctgccag
     1561 taaaaacact ttatgatatc ggagccaccc ttgacggtcg gcgcatcacc tctcctccaa
     1621 catcggagca aatcccttct ctgcgtctca acggtgatgt tctgaatgat tatctgtccc
     1681 gcacagttct gactccagaa caggctgata acataagaaa aatactgcac actcagggaa
     1741 tacgcagcgg tacccgtccc atagatccgg agatgattcg tgggacgcag gatgacctag
     1801 tttcgtcaca gactcgtctg caaaggcaag caacacgggt taaacagcaa ctcgccggtg
     1861 tacttgatac tctgcaacag cacttccaga acattccacg ttcatccggt cgtcatcttt
     1921 ctgtagagaa tattgagctg gctgatatcg gaagtgggcg tttcaacctt caaattcgag
     1981 atggagaaac attgcatacc acttctgtgg aagtaccgga agttgtgtcc cgttttcaga
     2041 aactttctac catgctttca gccctgcctg ccagtggaat catggatttc gacctcggca
     2101 tgagtgtggt cggagtcgtc cagtatgccc gcctgctaca gcaggggcac gaagacagta
     2161 ccctagccaa gataaatcta gctatggata tcaagcaact ttccgaagca actctcggca
     2221 gcatgattca gattgccggg aataagtttc tcaatacaga aggaatccag gggttcagac
     2281 tggaaagcgc cgtcgctgaa ggtatgcgct cagtagcaac ccgtaccgga ggcacaatgg
     2341 ggaaagccct ttctgccagt gcccgtgttc ttgaactgcc tgtactggaa acagttctgg
     2401 ggacatggaa cctgtacaac agcgtcattc agctccagca agctacatct tattctgaga
     2461 caatggctgc ccgggtacag attgcgtttg attccatttc tctgggatta actgccgctt
     2521 cggtagcttt cccgccacta attattgcca ctggccccat tgcggctatt ggtatgggag
     2581 cttccagtat tgcacgtaat gtggcacgga aagaagaacg gcatacacaa tggctggaat
     2641 ataaaaaatt cctgactgat ggcagtaaac acattgttgt ggcctctccg gaaagaggtc
     2701 tgctggattt ctccggaaac aaagtttttg gaaaaatggt gctggatctg cgtcagtctc
     2761 ctcctctctt gcatggagaa agctctttta acgctgaccg caaaatcggt catcgtccgg
     2821 atctggggga ctggcaaatt cgtgagaagg tggggtatgc caacagtatc agtccctact
     2881 cttctctggc gcacggttac gccaacagta aatggccacg aacaataccg aaaattccct
     2941 cgggagaata tgacaccata attctgggct acggtcacca gtatcaggcc aatacggaaa
     3001 tagaatatct gtctaactgg attgtatggc gggaagccgt accagacagt acttcccgcc
     3061 acaaacgtcc tcctctggag gttcttaata gtcagtgtac tgtgatagct ggagagcgta
     3121 aaaccacagt acttcccctg agagtgctca gcgatctgac accggaatgc acagaacagg
     3181 ctatatcgtt aaaagattat aaattcatac tgagaggggg aagcggtggg ctggctgttc
     3241 aggtcggtgg cgcgggatat tatgatattg atgcaaatcc tgtggcaaaa gaaaatacgc
     3301 tctcttttcg cgggctaccg gaagagtttc cgctcacctt tgatttatca aaacaaacac
     3361 agtcggtcat gctgaaaaca ccagacgatg aggtgccggt aatgaccatt acccagaagg
     3421 gaataaacac cctggtaggt acagccgccg gtaaagaccg actaatcggt aacgataagg
     3481 acaatacctt ccatacaagc tctggcggcg gtacagtcat ctccggaggc gggaataacc
     3541 gctatattat cccccgggat ttaaaaacgc cgttgacact gacgctgtcc agtaactcag
     3601 tctctcacga aatctttctg ccagaaacaa ccctagctga attaaaacct gtcgcctttg
     3661 agctgagttt gatttactgg gccgggaaca acataaatgt tcaaccagag gatgaagcaa
     3721 aactgaacca ctttgccgga aacttcaggg tgcatacccg tgatggcatg actctggagg
     3781 cggtttcccg ggaaaatggt attcaactgg cgatttcatt atgtgatgtt caacgctggc
     3841 aggctgttta tccggaagaa aataacagac cggatgccat actggacagg ctgcatgata
     3901 tgggctggag tctgacacca gaagtccggt tccaaggagg agaaacacaa gtcagctatg
     3961 atcccctgac tcgtcagctc gtttaccagc ttcaggcgcg ttactctgaa ttccagttgg
     4021 ccggtagtcg ccaccatacc acggctgtaa ccggaactcc gggaagccga tacattatca
     4081 tgaagccagt tacaacacag atattaccga cacaaatcat actggctggt gataatgacc
     4141 atccggaaac gattgattta ctggaagcta gtcctgttct ggttgaaggg aaaaaagaca
     4201 aaaacagcgt gatattaacg attgctacga ttcagtattc ccttcaactg acaatatccg
     4261 ggatcgaaga atcgctgccc gagacaaccc gtgtggcaat tcagcctcag gatacccgtt
     4321 tactgggtga cgtactccgg atcttaccag ataatggtaa ctgggtgggg attttccgga
     4381 gtggtcatac accaacggta aaccggctgg aaaatttgat ggcactgaat caggtaatga
     4441 cgttcctgcc ccgggtatcc ggaagtgcag agcaggtatt atgcctcgaa aacctaggtg
     4501 gcgtaaggaa aaaagtggag ggggagttac tgtcagggaa gctgaaaggt gcgtggaaag
     4561 ccgaaggtga acctactgtt ccggtaaata tctcagatct aagtatccca ccctattcac
     4621 gtctgtatct gatttttgaa gggaaaaata atgtgttgct acgcagtaaa gtacatgcag
     4681 ctccgttgaa aataacatcc gcaggagaga tgcagttatc tgaaaggcag tggcaacagc
     4741 aggaacatat tattgtcaag cccgacaacg aagccccctc attaatactc agtgaatttc
     4801 gtcgtttcac tatttcatcg gataaaacat tttctttaaa actgatgtgc catcagggta
     4861 tggtccgcat cgaccgcaga tcattatcgg tcagattgtt ctatctgcgt gaacagccag
     4921 gtatcggcag tttacgtctg acgttcagag attttttcac agaagtgatg gatacaacgg
     4981 acagggaaat tctggagaaa gagctgagac caattctgat aggagataca caccgcttta
     5041 tcaacgctgc atacaaaaat catctgaata tccagttagg agatggcgtt ctgaatctgg
     5101 cagacattgt tgcggaatac gcccgtattc aaaaggaaga aacatcaaaa atattgtatc
     5161 aatatcaagg tgccatgaaa aaaaaaacag atggaccatc tgtggtagaa gatgccatta
     5221 tgaccactac tgtcacaaca gattcaggtg aactattccc taccttccac ccgtggtata
     5281 cagatgattt atcagggcgt tataagagcg tacctatggc aagaaaagca gatactttgt
     5341 atcacctgac accaaaaggt gatctacaga taatatatca ggtagctaca aaaatggtga
     5401 atcaggcgat gattgtatcc ctgccaaact accgacacga gtgggaaaaa tataatttaa
     5461 gcatcttatc cgaaatccct cagaacaata atactgttgt acattcaatc ctcagggtta
     5521 atggccccac aatgcaggtg cgcacaattg actacagagg aacggatgaa aacaatccca
     5581 tagtatcttt ttcagataca accttcatca atggtgaaca gatgttgagt tatgactcgc
     5641 attcatcagg gcgagtctat tccagagaag aatatatgat gtgggaattg cagcaacggg
     5701 tatcagaagc ttccagtgcc cggacacagg attactggct gatggatgca gcggtaagaa
     5761 acggagaatg gaagatcaca ccagaattat tacgtcacac accgggatat atccggagta
     5821 cggtatcgaa atggtccaga ggatggctga aaaccggcac aatactccag actccagaag
     5881 acagaaatac ggatgtatac ctgactacca tacagaacaa tgtatttagt cgtcaggggg
     5941 gcggctacca agtgtattat cggattgatg gaatggctgg tgcggatata gcggataatg
     6001 caccagggga aacccgctgc accctcaggc ccggaacatg ttttgaagtg acaagtgtgg
     6061 atgaaaggca ttatgagtgg aatatcattt atgtcacgct gaaaacctgt ggctggagcc
     6121 gaaatggcca aagcaaaacg ccgaatggtg acaacctttt taactaatta aaatctctgt
     6181 taaagatgta taacccggca tctatgtgct caccagcata tagatgcaat gtgattcccc
     6241 tgatggtcgt atcagagatg gtatgcgagc ctgtacatag atttgtgtaa ttgcctgatt
     6301 ttgatatgtt caatccaaca tcaaaagcag gttaatttat ggacgaaaaa cagttgcagg
     6361 ctctggctaa cgaactggcc aaaaatctca aaacccctga agatctcagc cagttcgatc
     6421 gcctgctgag gaaaatcagc gttgaggcag ctctcaacgc tgaaatgtcc caccatctgg
     6481 gctacgataa aaaccagcct aaaccgggtg ccaactcccg caatggctat tccacaaaga
     6541 ccgttatcat cggtgatggc cccctggaac tacgcactcc gcgcgatcgt gatggctctt
     6601 ttgaaccgca acttgtgaag aaaaaccaga cccggatcac cgggatggat aaccagatcc
     6661 tgtcgttgta cgccaaaggc ctgacaaccg taagcgtaca gcctgaaccg tctggtcaga
     6721 atctgacgaa ttagagtaag cgtaaaccga
//
LOCUS       DAZFXT010000199         6698 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.199, whole genome shotgun sequence.
ACCESSION   DAZFXT010000199 DAZFXT010000000
VERSION     DAZFXT010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6698
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.199"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(481..693)
                     /gene="cspH"
                     /locus_tag="ACHKW0_004639"
     CDS             complement(481..693)
                     /gene="cspH"
                     /locus_tag="ACHKW0_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HFO7477318.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            979..1191
                     /gene="cspG"
                     /locus_tag="ACHKW0_004640"
     CDS             979..1191
                     /gene="cspG"
                     /locus_tag="ACHKW0_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HFO7477319.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            1202..1390
                     /gene="ymcF"
                     /locus_tag="ACHKW0_004641"
     CDS             1202..1390
                     /gene="ymcF"
                     /locus_tag="ACHKW0_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HFO7477320.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            1365..1595
                     /gene="ymcE"
                     /locus_tag="ACHKW0_004642"
     CDS             1365..1595
                     /gene="ymcE"
                     /locus_tag="ACHKW0_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HFO7477321.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFREM
                     AYNISYLTRGPGTNTLLACCCLLRPEEVNSEY"
     gene            1585..1758
                     /gene="gnsA"
                     /locus_tag="ACHKW0_004643"
     CDS             1585..1758
                     /gene="gnsA"
                     /locus_tag="ACHKW0_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HFO7477322.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1807..2880)
                     /gene="yccM"
                     /locus_tag="ACHKW0_004644"
     CDS             complement(1807..2880)
                     /gene="yccM"
                     /locus_tag="ACHKW0_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HFO7477323.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNNWRNATTWRKATQLLLLAMNI
                     YIAITFWYWVRYYETANSTTFVARPGGIEGWLPIAGLMNLKYSLTTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPTIGH"
     gene            complement(2952..5696)
                     /gene="torS"
                     /locus_tag="ACHKW0_004645"
     CDS             complement(2952..5696)
                     /gene="torS"
                     /locus_tag="ACHKW0_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HFO7477324.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQRQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALY
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDVPSALMGDPRRIRQVI
                     TNLLSNALRFTDEGQIVLRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPEPKTANQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDID
                     GITLARQLVQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQANTDQPLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMCSASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            complement(5791..>6698)
                     /locus_tag="ACHKW0_004646"
     CDS             complement(5791..>6698)
                     /locus_tag="ACHKW0_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7477325.1"
                     /translation="SDSGRKSLIDRIKGGDAITLHVWLDRYETILTERGIRPKTLLDY
                     ASKIRAIRRKLPDKPLTDISTKEVAAMLNTYVAEGKAASAKLIRSTLVDVFREAIAEG
                     HVATNPVTATRTAKSEVRRSRLTANEYVEIYHAAEPLPIWLRLAMDLAVVTGQRVGDL
                     CRMKWSDINDNHLHIEQSKTGAKLAIPLTLTIDALNISLADTLQKCREASSSETIIAS
                     KHYDPLSPKTVSKYFTKARNASGLSFDGNPPTFHELRSLSARLYRNQIGDKFAQRLLG
                     HKSDSMAARYRDSRGREWDKIEIDK"
BASE COUNT         1664 a         1646 c         1594 g         1794 t
ORIGIN      
        1 catgaaggcc ataaggattg cagaaagctt gtgtttcata acttttcctt tattcatcgc
       61 atggacaata cgtacgcttg agatcaggat tgtctacttt gagtataaca tccttgtgaa
      121 atctcccttt cctcataccg cttgcactat atacagcggg tgagaaaggc gcgcagaggg
      181 aaatataaga ttgttaacga tttccctctg gaaatacgac ttgtgagcat aagataaatc
      241 ttagaaaaat acaaattaaa gataagaaaa ttgttaagaa agctattaac tattgttgat
      301 aatatagttt caagcggtac tataactggc tgttaagtgg catttataac tattagttgc
      361 agagatattc ggttaaatgg attagtttcc tgataaaaaa gtaacttgcg ttataatgca
      421 tggtgttgac tattgaacta ttccaataaa accagtcagc tttaaacaag cagcgtcata
      481 ttaagagaga taaacatttg ccgctgttgg tcctcgcagg ccatttacgc gacaaaattc
      541 cacgcgtaat cctggtataa gcacttctgc gtcgcgggga gtgaatgcgg aaatatggac
      601 ctggacttct ttacgaccgt cggaggggat aatgaatcct ttgccgcttt tgcgatcaaa
      661 ggttttgaca attcctgtca ttttacggga caaacaaatt ccttactgaa aatactgcgc
      721 tgcactatac ggggttaata aaataaagcc agcgaaattt aagaccgccg gacggctaaa
      781 ataaaatttg cttaatctca attatcatgc gttaatagct gcgtcggttt gaaagacaga
      841 cagcatacaa agtagtttac taaagcagtt ctcattatca ggcattatcc ccttcttttg
      901 agtctctctc ctgaacacta agtagtttct gtattaaagc cctgtttgcc gaaaggccca
      961 aaatgaagga agtaaaatat gtctaataaa atgactggtt tagtaaaatg gtttaacgca
     1021 gataaaggtt ttggctttat cactcctgat gatggcagca aagacgtttt cgtccatttc
     1081 accgccatcc agagcaatga attccgcacg ctgaacgaaa atcagaaagt tgaattttct
     1141 attgagcagg ggcaacgtgg ccccgcggca gcgaacgttg ttacgctcta aggttgccgt
     1201 tattactcaa catctccatt tccgctgtcc atgttgtcat ggttcacagt accgcacatc
     1261 ggcattcgat gtgacggagc gaaacccttt gggcgctaag tgtatttttt gtaaatcgac
     1321 aatgatcacc tttgataacg tcgcgctgca aatacgcact gaccatgcgc cgctggattt
     1381 cacaaaataa tatcaggctc cctcgtggag ccttttttat atctgcctta tttttcttca
     1441 acgctgtatg tatagtaagc gataacctgt tgattattga atctttcagg gagatggctt
     1501 ataacatttc ttacctgacc aggggaccgg gaaccaacac cttactggcg tgttgctgtc
     1561 ttttaagacc agaagaggtt aacagtgaat attgaagagt taaaaaaaca agccgaaacg
     1621 gaaatcgccg actttatcgc gcaaaaaatc gccgagctga acaagaatac agggaaagaa
     1681 gtctctgaaa ttcgcttcac cgcacgggaa aaaatgaccg ggcttgaaag ttatgatgtc
     1741 aaaatcaaaa taatgtgatt ttgtgaacat caccccgtgc gaggtgatgt tccgcttgtt
     1801 gctaatttag tgaccaatcg ttggcgcttg tggaattaag cgtcggtaca attcctccgg
     1861 caccgggctt tgccatactc ccgcatacat tgcgtaacca atcaccgcaa acataatccc
     1921 cagaaccagt agtgtcatta accagccaga caacgcaaaa gcttttttat ttgccgcagg
     1981 tttttgcagt gaaaaggtca atgttgaggc taccggacat gactctacgc aagtcataca
     2041 gccggtacat tccactgttc gtacctgaat taatttatcg accgggatcc gtgatgggca
     2101 attttttgcg catttgccac agtcgataca actttcggca ttgcgacgaa tcttaaacgg
     2161 cgataatagc gaaaccacgc ccatcagcgc gccatatggg caaagataac gacaccaggc
     2221 atggcgaata aacaggctgg caatcagcaa aacggtcacg ctgattaatg tcgcggtccc
     2281 catatgacga aagaaatcga gcattttaac gtccatcacc acgctgtagg gcgacaacat
     2341 aaaatagtga atcgcctgag cgggcatcaa taacgcgata tagagaaaaa aactcaacag
     2401 caaatacttc acgccgcgca gaggaatatc cagccagcgg ggaaggacac attgccgacc
     2461 aaacagtttg ttaccgagat cgccgattaa ttcagaaagc gtaccaaccg ggcataacca
     2521 tgagcaaaag gcctttttga gtaatagact gatgacgata aaagcgacca ataacagcat
     2581 cgcggcggcg tggacggacg gtaactgacc tgtggtaagg ctatatttca gattcatcag
     2641 accggcaatc ggtagccagc cttcgatacc tcccggtctg gcgacaaatg tcgtgctatt
     2701 tgccgtttcg taatagcgca cccaatacca gaacgtgatg gcaatataaa tattcattgc
     2761 caacagtaat aattgcgtcg ctttacgcca ggtcgtggca ttgcgccagt tattccacgg
     2821 taatttgccg cccgtcgtgc ctggccgccg ctgccagcgg gttcttttat tctctgccat
     2881 gattttgcca gtccgttaag ttgcatacca aatgccacta ttctagttgt tcttaactgg
     2941 ctgatattga ttcaaatcgc gttcaggtct ttcttatgca accatgcttc cagagcggca
     3001 acactgcgtg taatttcttc gtgtggaagg ggggcagata atggctgctg ctccagttgt
     3061 gcgcatagct ggctggcact gcacattccc agactcgagc aactgctttt tagctgatgt
     3121 gcggcacgct ttattttctc gctatcctga ctggcgcggg caatgtcgat ttcatcgaga
     3181 agcggcaggg catgttgtgt aaataatacc agccattcgt ggatcttctc cgtccccatt
     3241 aactgagcat cttcattgag ttgcgataca tccagcggtt gatcggtatt ggcttgcagt
     3301 tggagatagt gcgccagtaa ctgaccgagc acttcacgcg gcaccggttt agggataatc
     3361 ccgcgaaata atgaactggt acgctggcgc aacgtttcgt cgatgacatg agcgctaaag
     3421 ccaatcaaaa ccagcgacgg atattgctgt accagttgtc gggcaagggt aatgccgtcg
     3481 atatccggca gatcaaaatc caccagtgcg gcagcaaacg gttcgctatt ttgcagtgtc
     3541 tctaaagcct gcgcggcatt gccaacagca acaacctgcg caccactggt gttcagcatc
     3601 tcaacggtaa ttcgctgggt tagcgggtta tcttcaatta acagcaaacg taaaccgtcc
     3661 agacgcaccg cctgattggc tgtttttggc tcgggtgcag tggcaacacg taacggcaag
     3721 cgtaaacaaa aacagcttcc aacctccggc gtgctggtgg cgctcagttc gccgcccatc
     3781 gcctgggcca gacggctact gatagtcagc cccagcccgg tgccgccgcg tttgccgctt
     3841 acctgcacaa atggctggaa gatttctgcc agtttcgcgg gatcaatacc gcagccgctg
     3901 tcttccactt cgaccagcca ttgctcgcca tcagtgcggc tacgtaaaac gatctgccct
     3961 tcgtcagtaa aacgtagggc gttgctcaac aggttggtta taacctgacg aatacgtcgc
     4021 ggatcgccca ttaacgcgct cggtacatca tcggcaattg ccgttgccag gcgaatcggg
     4081 cgacctttca cccgtccgct cattaattgc agggtacttt ccagcagcgg gcgcggttca
     4141 aagggttcat cgctgaccga aacattcttg ccgcctgctt cgatagccga ataatcgaga
     4201 atatcgttca ggatggtcag caacgattca ccagagtcag taattgcccg caaatcatca
     4261 cgctgggcgt taagtgcggg gttatctgcc agtagctgtg cggtgccgag aataccgtac
     4321 agcggtgtgc ggatctcatg gctcatcgcc gccagaaacg ccgattttgc ctggctggct
     4381 ttttctgctt ccgcccgtgc ctggcggtgt tctatcacca gttcctgcaa ttcagctgta
     4441 cgcgctttga cctgcgccgc cagctgttcg cggtggcgat tcagtgcatg aacattgctg
     4501 cgaaacgcat ccatcagccg cccgatggta tccagctccc gtacgccagc ggtttccggg
     4561 aaaggggagt caatatcacc gtccagcagc cgttgcagcg cctgcgtttg ttcggcaagt
     4621 ggacgcgtga ctgagcgata aaccacgcgc caaaggatca gaatcagtgc gcaaagtgaa
     4681 accatcccca gcaataacag gctgtattgc ccgcgtgcac tggctttttc cagatgcgcc
     4741 agtccgtgct gattacgcag ctcaatggtg tcgaccagct gactgacttc gctactaaac
     4801 tgcgcgaact gggcgatgtt attttgtgcg agagtttgta ggtgattgct gatttcactg
     4861 tcctgctgat acagcgccag caaatcgcta tattggctaa cggtagttaa cgttgttgcg
     4921 acctgcgcac gaacacccgg atcttcaatg cgtatttgcc gacgttgcag aattttcacc
     4981 gcattattga gctgcttttc cagcgttggt gcatttttct ggatctgctc cagccccaga
     5041 ttcatcacca tttgctgcac ccgcagagcg ctaaggcgca gttcattcat ctggttaaca
     5101 tactcaagat cgatatcaat cagccgatcg agtgcacttt cagcagcctg acgctgatct
     5161 tgttcgatca aatcgtaaat cccggcctgg gtcgctccag cggaagttgt cgcattattc
     5221 gcctgacctt gcgccaggcg tgcgatctca tcggcggcag cgactatctg ctgactgagt
     5281 tgccgttgtt gctggcgtag ttgcagacgc cgccccacca gttccccttg ctgacgtaat
     5341 gaacgggaga tctcctgctc ctgttgttca atagcggtgg tatcaaaacc ttgttcccgt
     5401 aacgcttgca gcaacgcatt aatcttcagg ctttgtgcgg tgagcattcg cccctgcgcc
     5461 tgccacatct tttcgttatc ggcactggtc aggttctgcg cggcgaaaag ttcccaggcg
     5521 ctggcttcgc tcaactggcg cgccatattc atggtaggaa tcaatgcctg agtgttgtct
     5581 ttttccacct ggctgataaa gcgcaggttg taccatccca ccagggtact ggtcagggtt
     5641 aacagcgcca tcagggcaaa gcccatccag agtcttcggg tcagggttaa attcacggtc
     5701 ggtgcactta aggtgaataa gttgtatgtt taaaatctct tttactatca atgaattagt
     5761 attggcaggt cattattggt caaaataaaa tcatttgtcg atttcaattt tgtcccattc
     5821 ccgtccacgg ctgtctctat accgcgccgc cattgaatct gatttatgcc cgagaagacg
     5881 ttgagcaaat ttatcgccaa tctggttccg gtatagcctc gctgacaggc tacgcagttc
     5941 atggaatgtt ggcgggtttc catcaaatga gagtccagat gcatttctcg cctttgtaaa
     6001 atactttgat actgttttcg gggaaagagg atcgtaatgc tttgatgcga ttatagtttc
     6061 actgctgctg gcctccctgc atttctgtag tgtatcagcc aatgagatat tgagcgcgtc
     6121 aatcgttagc gttagcggaa tggcgagttt agcccctgtt ttactctgtt caatgtgaag
     6181 atggttgtcg tttatgtctg accatttcat tctgcacaaa tcgccgactc tctgccctgt
     6241 aacgacggcc aaatccatcg ccagccttag ccagataggg agaggttcgg ctgcatggta
     6301 aatctcgaca tactcattag ctgtcagcct tgagcgcctt acttctgact ttgctgtacg
     6361 ggttgctgtt accggattcg ttgccacatg cccctcggct attgcttcac gaaaaacgtc
     6421 aacaagggtt gacctgatta attttgcgga agctgcttta ccttctgcta cgtaggtgtt
     6481 tagcattgct gccacttctt tcgttgatat gtcagtgagc ggtttgtccg gcaattttct
     6541 tcggattgcc ctgattttgc tggcgtagtc gagtagagtt ttcggcctga tccccctttc
     6601 ggtgaggatt gtttcatatc ggtcaagcca cacatgaaga gtgattgcgt caccgccttt
     6661 aattctgtct attagtgatt tgcgtccgct gtctgaga
//
LOCUS       DAZFXT010000200         6441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.200, whole genome shotgun sequence.
ACCESSION   DAZFXT010000200 DAZFXT010000000
VERSION     DAZFXT010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.200"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            16..1374
                     /gene="dgcT"
                     /locus_tag="ACHKW0_004647"
     CDS             16..1374
                     /gene="dgcT"
                     /locus_tag="ACHKW0_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HFO7477326.1"
                     /translation="MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYL
                     MVMIALFFIDTVAFIFMQLYFIYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGR
                     LTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFW
                     GPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRY
                     DIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALR
                     VTKNIAHRDPLTNIFNRNYFFNELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVG
                     DQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGD
                     NPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCE
                     SP"
     gene            complement(1415..2577)
                     /locus_tag="ACHKW0_004648"
                     /pseudo
     CDS             complement(1415..2577)
                     /locus_tag="ACHKW0_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
     gene            2644..3171
                     /locus_tag="ACHKW0_004649"
                     /pseudo
     CDS             2644..3171
                     /locus_tag="ACHKW0_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
     gene            3168..4154
                     /gene="ycdU"
                     /locus_tag="ACHKW0_004650"
     CDS             3168..4154
                     /gene="ycdU"
                     /locus_tag="ACHKW0_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcdU"
                     /protein_id="HFO7477327.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            4521..5723
                     /locus_tag="ACHKW0_004651"
     CDS             4521..5723
                     /locus_tag="ACHKW0_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7477328.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVAIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYTDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARI
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQIVNK"
     gene            complement(5910..>6441)
                     /locus_tag="ACHKW0_004652"
     CDS             complement(5910..>6441)
                     /locus_tag="ACHKW0_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477329.1"
                     /translation="DINYFMTYQGESINLISLDHEYPESCLQNDTNTYLLGRVIFLYN
                     SMIYKFINCQEHETNNIHSAMINNLLQEVDIALGKINDIIDSRNISAPHELANILTRE
                     KILTTREKKGNLISLFDGFTLFHCVGMITFLIHYLRTPEEKVENIFMLYGADKNNKLR
                     RRLIYDALGIIQSQQE"
BASE COUNT         1649 a         1293 c         1550 g         1949 t
ORIGIN      
        1 gattggtgta gctttatgga aaaagactat ttgagaatta gtagtactgt attagtgagc
       61 ttattgtttg ggcttgcttt ggttttggtg aatagttggt ttaatcagcc aggcgttgag
      121 gaagttgttc cacgatcaac ctatctgatg gtaatgattg ctttattttt tatcgatact
      181 gttgcattta tttttatgca gttgtatttc atttatgacc gtaggcaatt ttcaaactgt
      241 atacttagtt tggcttttct gagttgtttg atttactttg ttaaaaccgt cattatcatt
      301 cagcaaatta ttgaggggcg tttgacaagc agtgttgtcc aaaatgacat tgcaatctat
      361 tatttgtttc gtcagatgag tttgtgcata ttaatatttc tggcattggt gaataaagtt
      421 agtgaaaaca caaaacagcg caatttattt tccaaaaaaa tgactttgtg cataagtttg
      481 ttttttgttt tttgggggcc gatagttgct cacatacttt ctagtcatta tgagagctac
      541 aatttacaca ttgccgagtt aaccaatgaa aatggtcagg tggtttggaa agcctcatat
      601 gttaccataa tgattttcat gtggttaacc ttgctttcgg ttaatctata ctttaatggt
      661 ttacgctatg atatctggaa tggagtgacg gtgattgctt tttgtgccgt actgtacaat
      721 atctctctgt tatttatgag taggtatagc gtgtccactt ggtatattag tcgcactatt
      781 gaagtagtca gtaagttaac cgttatggta atatttatgt gccatatatt cagtgcgcta
      841 cgagtaacaa agaacattgc acatcgcgat cccttaacca atatatttaa cagaaattat
      901 ttttttaatg aactgacagt tcaatcagca tcagccaaaa aaacgcctta ttgcgtcatg
      961 attatggata tcgaccactt caaaaaagtc aacgacacct gggggcatcc ggttggcgat
     1021 caggtgataa aaacagtggt gaatatcatt gggaaaagca tacgaccaga tgacctttta
     1081 gcgcgcgtcg gcggcgaaga gtttggcgtc ttgctgacgg acatcgatac tgaacgtgcg
     1141 aaagctttag cggaaaggat tcgggaaaat gttgagcgtt taactggcga taatcctgaa
     1201 tacgctatac cacaaaaagt gacgattagt attggcgctg ttgtgactca ggaaaatgcg
     1261 ttaaacccga atgagatcta tcgactggct gataacgcac tttacgaggc gaaagagacc
     1321 gggcgtaata aggtggttgt gagggatgtg gtgaattttt gtgagtcacc ataaagcggc
     1381 attttgatcc tacccacgta atatggacac aggcctaagc gaggttcttg ttttcaaatt
     1441 gttccggact gaggccgcca caccaactgt gccgccgcca ccgattgtaa tcacattcga
     1501 tataattaaa caccgttgcc cgcattattt cccggctgat aaagtgttct ccatggatac
     1561 attccacttt cagcgaatga aagaagcttt ccacgcaggc attatcgtag cagcaacctt
     1621 ttgcgctcat acttccacgc agattatgcc gcttcagttg cgcctgataa tctgctgaac
     1681 agtactggcc tccacggtcc gtgtgaacga taacgttccg gggcctctta cgccgccaca
     1741 gcgccatctg cagggcatcg caggccagtt gcgccgtcat gcgtggcgac attgaccagc
     1801 caataacggc acgtgaccac aggtcaatga ccactgccag atacagtcag ccttcatctg
     1861 tacgtaagta cgtgatgtct cctgcccact tctggttcgg gccactggcg taaaaatcct
     1921 gctccaacag attttctgac acaggcaggc cgtgtgcgcg gtagctgacc gggctgaact
     1981 tccgggaggc ctttgccctc agtccctgac ggcgcaggct tgccgccacg gtttttacgt
     2041 taaaggggta cccctgagca cgcagttcat ccgtcaggcg tggggcaccg taacgctgtt
     2101 ttgaccgggt aaaagccgcg aggacaacgc tgtcgcagtg ttggcggaac tgctgacgcg
     2161 tgcttatcct tgtccgccgc tgacaccacg tataccagcc gctgcgggcc acccggagca
     2221 cgcggcacat tgctttgatg ctgaactcag cctgatgttt ttcaataaag acatacttca
     2281 tttcaggcgc ttcgcgaagt atgtcgcggc cttttggagg atagccagct cttcatcccg
     2341 ttctgccagc tggcgtttga gacgtgcaat ctcggtagac atctccagtt cacgttcaga
     2401 agacgtctgc tgattttgct gtttactgcg ccagttgtag agctgtgatt catacaggct
     2461 gagttcacgg gctgcggcag taacaccgat gcgttcagca agcttcaggg cttcactgcg
     2521 aaattcaggc gaatgctgtt tacggggttt tttactggtt gatactgttt ttgtcatgtg
     2581 agtcacctct gactgagagt ttactcactt agccgcgtgt ccactattgc tgggtaagat
     2641 caggcggtta aaggcatcaa ccgcgaacaa ctgcagacga tgattacgga atcaggcatt
     2701 gcgctggata ttagctgtga taacacgccg cgccagcagg ttattggggg aacgcaggct
     2761 gcgttgaatg aatttgccac gctgctgatg gccgctggat acgaaccggt taaactcggt
     2821 gtgagcggtg cctggcacac gcgtttgatg gaagacggcg ttcaggcaat gcgcgattat
     2881 cttgccggac tggatatcgc ttcgccagag catcaggttc tgatgaacgt aacagcaaaa
     2941 agcgaggtcg ctccgtcaat tatcaaagaa aatctctcat tacacttaac tcatacggtg
     3001 aaatggactg aatctttcga tacatttctg aatatgccaa ccccggtagc tttccttgaa
     3061 ataagtaata agccctattt ggggaatatg ttaaatgatt ttgctggcgt ggttcagcaa
     3121 cgagttatgc attgtcgtaa agcatttagt gatgcaaagg tattcaaatg attcctgatt
     3181 atttaacttt tattcgcttt caggataaac gaaatctgat atacatttat gctattggac
     3241 ttattctgat aggcttttat tggaagaatg cagggtttac ttttccatca gaggatattg
     3301 gtgtagttag tgggattctg gccctggtgc tgtataattt tatttttgat ctcaaggcgt
     3361 actgggctta taaatgcgtc acgaagaata tcgatttttc gtggtttaag aaaaagcaga
     3421 accacaaaat agaattattt cttacacaac ctctggtggc aggatttctg tcgttaatca
     3481 tgttgagtgc aatgagttgg gggctatacc agcttctacc ctcgttatat gcgctgttcc
     3541 tgatttcgtt acttgggccg ttggtcatct ttctgctgtt tcggatgatc cgcaccagtt
     3601 atgtcaagca ggtcgctatt tcagtagcga aaaaagtaaa atataaaagt ctgactcgct
     3661 atgtgctgct ttcggtgtgc atctcaacgg ttgttaacct gcttactatc agcccgttgc
     3721 gtaacagtga ttcttttgtg acagaggggc agtggttaac gtttaaatcg ataattgcat
     3781 tgctcattct ttgtggcgta gtgttggcga ttaatctgtt ttttctgcgc ttctccaagc
     3841 ggtacgcttt tctgggcagg ctttttttgc aggaaatcga tctgtttttc tccagtgaaa
     3901 atgcgttgtc gacctttttt gccaagccgc tttggcttcg gttattcata ttgctggtta
     3961 ttgaaatgat gtggattacg ctggtgtcgg tattggcaac gcttgtggaa tggcggattt
     4021 ggtttgaagc ctatttttta ctctgctatg taccgtgctt aatttactat tttttccatt
     4081 gtcgattcct ctggcataac gattttatga tggcatgtga catgtatttc cgttgggggc
     4141 attttaataa gtgaggaagt tacaggaagt gaccagataa cgcatatatg ttctctacta
     4201 tcttgcgcat tttgattgtt gactgagtaa ccagacagtt gatgtgcaca atttcccctc
     4261 gccctaacag acgtgggcgg gggcacccgg tgtgtgcaat atacgaaaaa aaagcccgta
     4321 ctttcgtacg ggctcttctt taaatatggc ggtgaggggg accgcctgaa ataaatctga
     4381 cttaatccag gggtgttata taatttcatt ataaacaatg tattacactt tttgttgtcc
     4441 gatattatct aatgtattct tagtgaagcg agagactaat ggcatactgg atggcatacc
     4501 agagtgacgg gaattagcgt atggcagtat tgacggatac gaaagcaaga catatcaaac
     4561 ctgatgacaa accattgccc catgggggaa ttaccggact gacccttcat ccttcttcag
     4621 taaaggggcg ggggaaatgg gtttttcgtt atgtaagtcc ggtgacacaa aaaagacgta
     4681 atgctggatt gggaacttac ccagaggtca gtattgctga agctgcacgt actgcccgga
     4741 taatgcgaga gcaacttgct gcaggtgatg atcctctgga gattaaaaag gctgaatctg
     4801 agaaagtcgc tatcccaaca tttgccgatg cagccaggcg tgtacatgca gaactgtctc
     4861 ctggatggga aaatccaaag catgtaaggc agtggttatc gacgcttgag aattacgcgt
     4921 ttcctcaact gggagcaaaa acgctggatt cgattacggc tgcggacgtg gcagaaacac
     4981 tgcgtccagt ctggttaacc ttgtcagaaa cggcaagccg ggttaaacag cgcattcatg
     5041 ttgttatgca gtggggatgg gcgcacggtt tttgtgtagc aaatcctgtt gatgtggttg
     5101 accatttgct tcctcagcag acaagaggac gtgatgaaca ccaacccgca atgccctgga
     5161 ggcagttacc gctttttgtg gcgaccagtg tgtataccga tgaaccttat aatgttaccc
     5221 gcgcactgtt attaatggtg atacttacag caactcgctc gggcgaagcc aggggaatgc
     5281 gctgggctga aattgatttt cataagcggg tatggactat acctgcagaa agaatgaaag
     5341 ccaggataca gcatcgtgtt cctttatccc ggcaggctat ttacattctg gaaaatatac
     5401 gtggcctgca tgatgaactg gtgttccctt cacccagaaa gcagcagatc ctttccgata
     5461 tggtgttgac aagttttctg cgtaaaaaga aagccgtcag tgacattccg gggcgagttg
     5521 ccacggcaca tggttttcgc tcaacattca gggactggtg tagcgaacag gggtattcgc
     5581 gggatctggc ggaaagggcg ctcgctcata cgctgaaaaa taaggttgag gcggcatatc
     5641 atcgtactga tctactggag cagcgtgtac cgatgatgca ggcatgggcg gattatgtga
     5701 tgtctcaaat tgtgaataaa taatagtgaa aatgtatatt gtacgatcat gatttgatat
     5761 gtgatcgtag cagtagtaat taagaaaccg cttttatgtc aaatgtattg agctgataat
     5821 tatcagatcg tcaggaaagc gtattttgat taacagggga ttacttaaca atcccctttt
     5881 tataaaaaat aatttgcctg tttaactctt cactcctgct gagactgaat tattcctagt
     5941 gcgtcataaa tcagtcttct gcgtagttta ttgtttttat ctgcaccata taacataaat
     6001 atattttcaa ctttttcttc aggtgttctg agataatgga taagaaaggt tatcattcca
     6061 acacaatgga ataaagtgaa tccatcaaac aggcttatca ggtttccttt tttttcccgt
     6121 gttgtaagta ttttttcgcg ggtaagaata tttgccagtt catggggggc tgatatgttt
     6181 ctgctgtcta taatgtcatt tattttacca agggctatat caacttcctg taataggtta
     6241 tttatcatag ctgagtgaat gttattggtt tcatgttcct gacagtttat gaacttataa
     6301 atcattgagt tatacagaaa tattactctt cctaataaat atgtgtttgt gtcattttga
     6361 agacaggact ctgggtattc gtgatccagt gaaataaggt taatggactc gccctgataa
     6421 gtcataaaat aattgatgtc a
//
LOCUS       DAZFXT010000201         6424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.201, whole genome shotgun sequence.
ACCESSION   DAZFXT010000201 DAZFXT010000000
VERSION     DAZFXT010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.201"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(113..1651)
                     /gene="cadC"
                     /locus_tag="ACHKW0_004653"
     CDS             complement(113..1651)
                     /gene="cadC"
                     /locus_tag="ACHKW0_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HFO7477330.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPVKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(1911..2025)
                     /locus_tag="ACHKW0_004654"
                     /pseudo
     CDS             complement(1911..2025)
                     /locus_tag="ACHKW0_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218818.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(2400..2972)
                     /locus_tag="ACHKW0_004655"
     CDS             complement(2400..2972)
                     /locus_tag="ACHKW0_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HFO7477331.1"
                     /translation="MSEITVSRPEVVNENTDVICSTSVRSRSLEYDNFPEISEANILS
                     TFEQLHQNKDEVFERGVINVFKGLSWDYKTNSPCKFGSKIIVNNLVRWDQWGFHLISG
                     MQADRLADLERMLHLLSGKPIPDNRGNITINLDDHIQSVQGKGRYEDEMFIIKYFKKG
                     GSAHITFKRLELIDRINDIIARHFPSVLSA"
     gene            complement(3069..3266)
                     /locus_tag="ACHKW0_004656"
     CDS             complement(3069..3266)
                     /locus_tag="ACHKW0_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HFO7477332.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNNEDKTDSAALLPC
                     IEQAREDIRTLRQLQLQHQNR"
     gene            complement(3278..3766)
                     /locus_tag="ACHKW0_004657"
     CDS             complement(3278..3766)
                     /locus_tag="ACHKW0_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5983 family protein"
                     /protein_id="HFO7477333.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLTELINNVAENGYS
                     LRVVEESDQQSTCTPPPFATLAGIRCSTAHITETDNAWLYSLSHQTRDVGESEWIHFT
                     GTGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLTRRYGVSLIHLDANAECLPGLPTF
                     DW"
     gene            complement(3763..4140)
                     /locus_tag="ACHKW0_004658"
     CDS             complement(3763..4140)
                     /locus_tag="ACHKW0_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system toxin CbtA family protein"
                     /protein_id="HFO7477334.1"
                     /translation="MKTLPDTHVREASSCPSPVTIWQTLLSRLLGQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPEFSACTRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYQEAKR"
     gene            complement(4230..4598)
                     /locus_tag="ACHKW0_004659"
     CDS             complement(4230..4598)
                     /locus_tag="ACHKW0_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HFO7477335.1"
                     /translation="MSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRHQHTVTLYAKGLTCKAD
                     TLGSGGYVYLAVYPTPETKK"
     gene            complement(4761..4982)
                     /locus_tag="ACHKW0_004660"
     CDS             complement(4761..4982)
                     /locus_tag="ACHKW0_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HFO7477336.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(5045..5521)
                     /locus_tag="ACHKW0_004661"
     CDS             complement(5045..5521)
                     /locus_tag="ACHKW0_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HFO7477337.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLITERLVQALGLVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(5537..6022)
                     /locus_tag="ACHKW0_004662"
     CDS             complement(5537..6022)
                     /locus_tag="ACHKW0_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7477338.1"
                     /translation="MKTISHNSTTSSVSVTAASGNDQPQLVATIVPDEQRISFWPQHF
                     GLIPQWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFIAPEPDDDDGETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTEYYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(6077..>6424)
                     /locus_tag="ACHKW0_004663"
     CDS             complement(6077..>6424)
                     /locus_tag="ACHKW0_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7477339.1"
                     /translation="VFDRIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTA
                     DILTPRRREDYGKDLWSAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRA
                     LWVMAETLLESLR"
BASE COUNT         1628 a         1480 c         1618 g         1698 t
ORIGIN      
        1 ttatgtgata aaaattatgc ggcaaataaa ttgccgcaac atattatacc aatagaaaca
       61 tacaaaaact caacaacaaa tattcccgag cataaatcaa cccgggagtt acttattctg
      121 aagcgagaaa tttgtcgaga taaggtacaa cataaggaac agaagtctgg aatataccat
      181 tttcaatcca gtaaagggtg tttgcccctg ggcgtaaatt aaaggcggtg agatatgcat
      241 cagccgcttc ccggttcatc cccttcattt cataaacctt gccaagcaac acataattta
      301 gccaggacat ttcaagatca atgccagtat ttatcgcctg gtaagactca tctgttttac
      361 cttttaccag agcactgacc gcttttattt gatatataat ggacaggtta ttcaattccg
      421 gcagtgtaac aatgttgtct atttctgtgt tcagtgctgc taattgtttt tcatctaaag
      481 gatgttgaga atggcgcacg atatcaacta atgctttttc tgctctcgcg taggtaaatt
      541 ctggggatga ttgaacaatc tcacctaata attcactggc acgattcaat gatttatcat
      601 cgccatgcag taaataatca tgtgcctgat aaaaattagt taataacgca ccacgatgag
      661 gcaatatttg ctggagcgtc tcctgcattc gttgtggcca cggttggttt aacgcttttg
      721 ataaactctc cagtaaatca ttttgaatcg ccagctgatt accgttagtg atgacataac
      781 gtttatccag catggttgaa ccatctgcat tgtctaccaa ttttatcgac ataaagcatt
      841 gttgagcacg gtattggcgc tgattaacaa acgcaataga taatgtttta ccggaactgc
      901 tcggttcatc aatgttgtag ttgattttgt catgcaccat aaaggtggag aaggtgttaa
      961 gtgatgtcgc caccaaatca cccacgccta tcgcgtaaga gagctgatac ggggaactcc
     1021 agctgttaca acttttattt accatattaa tgtcaatatc gcgtggattg agcaaaatac
     1081 gcgatttgct cataggaaga cgtgtatcaa gagttgaaaa cgctaccagt gctacacaga
     1141 tacctaacga caacaggaaa aaaaaccata cccaaaaggt agtgaatcgt ttgcttttaa
     1201 ctggggattg ttcaggtggc gttgcggtgt tttgaatgtt aagactgtgg gagggagaat
     1261 ctgtggcagg aaccgcctct ggtatagggg gaggcgaaga tagcattatt tcctctccct
     1321 cttcttcgct gtaccagata accggcacca ttaatttata gccgcgcttt ggtacagtag
     1381 cgatatagac aggactatct tcatcattat cttttaatga cttacgtagt tctgagatac
     1441 tctgcgtcac aacatgattg gtgacaatac ttctcttcca gacattatcg ataagttcat
     1501 ccctgctaag tacttcgcca ctgtgttgag caaagaaaac cagaagatcg attaatctcg
     1561 gctcaagggt aagttgacgc ccattgcggc taatttggtt tatggacgga gtaacaagcc
     1621 attcgccaac gcgaactaca ggttgttgca taatagaaac tcattcgaaa agggaatgat
     1681 gcaatgataa ttgccacaac ctatttttac catctataga tgggtttatt tacatattat
     1741 tggtgaatgc aagacgttat ttttaccagc cataaacttc ttgattacat agtattacga
     1801 aaggatttta ctgagaacca gaagtaatat tccttaccat caaaattcat catctttgcc
     1861 aaagaaaaat gttcagaaaa taatccatgg aaaattgtcc ggagcgctta ctattttaat
     1921 ggattgttag tctttgcatg agcaagcgga ctgatacatt tctctttgtt ctcattcaga
     1981 aaatctcatc agtcggcgtt cagaccggtg ttgtgcttta tggatgcgtt acgctcctga
     2041 tgacgtcatt tgacgttcaa caacatcacg gggccgcacg acatttcacg tcagttagtg
     2101 ctatcgctca ggaacaaatt ttcccgaatt gggatatgcc cgcaaattgc tggtgatgtg
     2161 gggagaatct ggttgagttc gggagaattg atttggaggc agaacgctta aattgtggcg
     2221 tccagaaacg aaaaacggac ctccgtggag gtccgttgat atgaatttgg tgccggactc
     2281 ggaatcgaag aatgcttcta tcatgttgat attattttat tttgtttggt ttggtgaaat
     2341 atgtaccctc ttttgtaccc gcagtccctt ttgatatcga aagggaatca aactcggggt
     2401 caggctgaga gcacagaagg aaagtgcctg gctattatat cgttaattct gtcaatcagc
     2461 tccagccttt tgaaagtgat gtgtgcagat ccacccttct taaagtattt aatgatgaac
     2521 atctcatctt catagcgtcc tttaccctga acggactgta tgtggtcatc cagattaatg
     2581 gtgatattcc ctcggttgtc ggggatcggt ttaccgctga gcagatgcaa cattctttcc
     2641 aggtcagcca ggcgatctgc ctgcattcca ctgataagat gaaatcccca ctggtcccat
     2701 ctcaccagat tgttgacgat aattttactg ccaaatttac agggtgagtt ggttttgtaa
     2761 tcccagctca gccctttgaa gacgttgatc actccccgtt caaacacttc atctttgttc
     2821 tggtgcagtt gttcaaatgt gctcagaatg ttcgcttcgc tgatttccgg aaaattatca
     2881 tattccagtg acctggacct gactgaggtg gagcagataa cgtccgtatt ctcgttgacc
     2941 acttccggac gggagactgt gatttcagac ataatcgata ttcctttttg cagataaatg
     3001 aaaacgccag ccgtgttcag gctggcgtca gtggaatgaa gtctggtgag tgagatgatg
     3061 agtgagactc accggttctg gtgctgaagc tgcagttggc gcagggtacg gatatcctct
     3121 ctggcctgtt cgatacaggg caacagtgct gctgaatccg ttttatcctc attgttgtca
     3181 aagataattc ccgattcgca gtcgatattg tcctgcagcc acgcaatcag aatatccagc
     3241 gctgtttccg tggttaatga tttcatgttg tttctggtta ccagtcgaaa gtgggtaaac
     3301 ccggcaggca ttcagcgttg gcatccagat gaatgagact gacgccataa cgtcgggtaa
     3361 gtgtgacaac cagacgacgg aacgtttttg acaggcccag gcgcttaagc cgcagaaccg
     3421 ggtacgacca cgcatcagta cgtaacagat aaccggtacc tgtaaaatga atccattctg
     3481 attcaccaac gtccctggtc tggtgtgaca gcgagtacaa ccaggcgttg tccgtttccg
     3541 tgatatgtgc ggtactgcag cgtatgccgg caagggttgc aaatggcgga ggtgtgcagg
     3601 ttgactgttg gtcggattcc tccaccacac ggagtgaata accgttttca gcgacgttat
     3661 taatcagttc agtgagatta acaccatcga cgtcaacgac aatgcgcccc atgttcagtg
     3721 cctgcacgtt aacgctgtca gcttccagcg tcagggccag tttcatcgtt tcgcctcctg
     3781 atacttaccc agggtaatgt tatttaccgt tcggtaattg tcgcgggtca tcaggccagt
     3841 cgcccggcga gcccggagga tatcgatgct gtttattaac tgagagcggg tacaggcact
     3901 gaattccggc tggtcggtac gcaccagtgc gtatttttca acaagaaagt tcactgcatc
     3961 gcacagtgaa atgccagctt caatatgctg ctcaatcaca cgttcatcgg cgaacggtgt
     4021 gtcattcagt gtaaggccgt aatgctggcc cagtagtcgg gagagcagag tctgccagat
     4081 ggtgacggga gacgggcagc tcgatgcctc ccgtacgtgt gtatcgggta atgttttcat
     4141 actgaaggtt ttcctgatat gcagagatga aaaatggaaa agtggcgtgg tgaaagcacc
     4201 aggccggagc agaaggttat tccggtggtt tactttttcg tttcgggcgt cggataaaca
     4261 gccagataaa cgtaaccacc gctaccgaga gtgtcagctt tgcaggtcag accttttgca
     4321 tacagcgtga cggtatgctg atggcgggga ttcagttcac cgctggtgag catgagttcc
     4381 agttgtttca tcagcagcgg aaaggcctgg tccaggtggt acgcatctgc atcgctgaac
     4441 cggcctctga taccggcacg gtcggcaagg taatgcaacc ggttaccctc ctgcaccaga
     4501 cgggcgccga aacagggcgt cactgtgcag ggtagtcccc accagggatg gtcgtcattg
     4561 tcatcgggat gcgttgtccc ggaaagtgtg tctgacacga taaaatcctc acagaaaatc
     4621 ggtgaatgat agtttaacga tgacgggtca gccggcgcgc agaccggctt cggtaaaggt
     4681 gtattcgttg atgagcagga cctcatccac agcggcgtct gagctcagcc agtcgtactc
     4741 gttttccggg tgacggaaca tcagttcagg gtgacgctca tcagccggac atacggacca
     4801 aaactgtcct tacggcgttc tgcaaacacg gccagcacac cgggaatatc ctgcacttca
     4861 cgaccggtat acgcttcagc actgccgtgc cagcggtatt tgccggtaca gaacggaaaa
     4921 agacgggatg ccggatgctg acggtgaata cgcatggctt cgccacgggt gatgattttc
     4981 atagtgggat acctctgaag acagaagata aaagtgaaaa caggtgtgat gtggttgtga
     5041 cgggttaaag cagaccatgt tcggcaaagg agaaaacctg gctgccaccg actatcagat
     5101 ggtccggcac ccggatatcc accaggccca gtgcctgtac cagacgttcc gtgataaggc
     5161 ggtctgcctt gctgggtgtg acttcaccgg acgggtgatt gtgtgccagc accacagcag
     5221 cggcattgtg atacagggcg cgtttaatca cttcacgggg atggacttcc gtgcggttga
     5281 tggtgccggt gaagagggtt tcaccggcaa tcagctgatt ctggttgttc agatacagca
     5341 cccggaactc ttcacgctcc agtcccgcca tgttcagaat cagccattcc cgtgccgcac
     5401 gggtggaggt gaaggccaca ccgggttcat gaagatggcg gtccagggtt ttcagagccc
     5461 gcagaatgag actgcgctcg ccgggcgtca tttctccggg cagaaaggaa agctgctgca
     5521 ttgtccctgc ctccattcag tcgatgatgc gcataatggc gctgcattcc ggatgctgca
     5581 gggcgtaatc ccgcagccgg taataatgga ccgtcatggc ataatactcc gtacgacagg
     5641 cgtgatgact gtacgtcatc agacaggcag caatgccggc ggcttccggg ctcatttcag
     5701 cgcggttacc gttcatggca ttgaacagta cccatgtttc gccatcatcg tcatccggtt
     5761 cgggcgctat aaatgcgcca ccgttgttca gtgtgtacag attccagata ccaccgcagt
     5821 agtcttcgca aagacggtcc atccagccga agatacgggg ctccagggtc acccactgtg
     5881 gaatgaggcc aaaatgctgc ggccagaagc tgatgcgctg ttcatcaggg actatggtgg
     5941 caaccagctg aggctggtca ttccctgatg cagcggttac ggaaacagaa gatgtggtgg
     6001 aattatgtga aatggttttc atgaaatgac tccttaaagg acaaataaat gcgtggtttt
     6061 cagggtaatg gctgaatcag cgcaggctct ccagcagggt ttccgccatc acccacaatg
     6121 cgcggttgag cttaatgtca gtgtcaatgc tgtgaatggc acgggtatgg atacgttttc
     6181 ctttagcgct gcgacctgaa attccgcctt tcagcatatt ctcctgaatg gtctgataag
     6241 cactccacag gtccttaccg taatcctccc ggcgtcgtgg cgtcagaatg tcggcggtgg
     6301 tgacgggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc agcgcctggc
     6361 gtgccggtgg cggcagaatc agcgactgca tggcatcacg cttttcctca atccggtcaa
     6421 aaac
//
LOCUS       DAZFXT010000202         6381 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.202, whole genome shotgun sequence.
ACCESSION   DAZFXT010000202 DAZFXT010000000
VERSION     DAZFXT010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.202"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            680..814
                     /locus_tag="ACHKW0_004664"
     CDS             680..814
                     /locus_tag="ACHKW0_004664"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477340.1"
                     /translation="MPHPERNHCLLLMGYLHGVQASELLGIKLSDIDLQAGNLNIRRL
                     "
     gene            1419..2240
                     /locus_tag="ACHKW0_004665"
     CDS             1419..2240
                     /locus_tag="ACHKW0_004665"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HFO7477341.1"
                     /translation="MLNARHLPVLMYHHVSRCPGLVTLSPETFCEQMKWLADNNWRTV
                     TSAELEYFYQGGTLPRKSVMLTFDDGYLDNWLRAWPVLKEYNLHAHIFLITGLIGKGN
                     VRSRQEQEYSHRDCERLIADNRSDEVMLRWSEVREMRDSGLVEFHVHTHSHKRWDRLS
                     VSRAEQCRLMKEDILVGKQCLTEKLGFCSSHLCWPEGYYNRDYINLAGKLGFSYLYTT
                     ERRMNCPENGSLRIGRISTKEREHSGWLKRRLFYYTTPLFSSVLALHKGPRLPDN"
     gene            2240..3346
                     /locus_tag="ACHKW0_004666"
     CDS             2240..3346
                     /locus_tag="ACHKW0_004666"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HFO7477342.1"
                     /translation="MMKILFTESSSDIGGQELQALAQMTALQKQGHSVLLACREKSKI
                     APEARKRGHDVTFIPFRNSLHLPSILRLRRIIGEFKPDLVICHSGHDSNIAGLSRLIC
                     CHRFSIVRQKTYITRKTRTFSLNYLCDFIVVPSSAMMAHLMAEGVRTPVTVIPPGFDW
                     PALHNEAMRPLPLHIHAWAASADNVPLIVQVGMLRPEKGHEFMLRVLYQLKMEGKSFR
                     WLVVGAGREEYEARLRQQTEHLGMSGDVLMAGALFPALPVYRIASVVVMPSENEAFGM
                     VLAEASVSGVPVIASETGGIPDVIQKNVTGTLLPVGDVSAWTGALRDFLSRPERFRMM
                     AASAREDIECRFDINRTAQIIVSLASQAKGKCNR"
     gene            3436..5157
                     /gene="cptA"
                     /locus_tag="ACHKW0_004667"
     CDS             3436..5157
                     /gene="cptA"
                     /locus_tag="ACHKW0_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HFO7477343.1"
                     /translation="MHLNTGQNRPTFSWSALGWAIFYFGFFSTLLQVIIFSSGYSGTN
                     GIRDSLLFSCLWLIPVFLYPDRIKIIAAVVGFILWGTSLAALCYYFLYGHEFSQSVLF
                     VMFETNAREAGEYFSQYFSLKLLLISLVYTAVSVFLWTRLRPVYIPLPWRRIVSFLLL
                     YALLLHPVVLKSLIRQEPLNDTLGKLASRMEPAAPWQFVSSYYQYHQQLNALTTFLNE
                     NSALPPLGNLRDESGERPRTLVLVIGESTQRERMSLYGYLRETTPELDALRKTDPGLT
                     VFNNVVASRPYTIEALQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTIT
                     ARNTMLTVFSRQTDRQYYMNQQRTQSAREYDTNVLKPFREVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPEGQGRFDGITGHIPTGLNAKELEVYNDYDNANLFNDHVVASLIKDFRAT
                     APDGFLLYFSDHGEEVYDTPPYKTQGRNEDNPTRPMYTVPFLLWTSEKWHAAHPRDFS
                     QYVDRKYSLAELIHTWSDLAGLTYDGYDPTRSLVNPQFRETPRWIGNPYKKNGLTDFD
                     TLPYGEP"
     gene            5231..6229
                     /gene="lpxM"
                     /locus_tag="ACHKW0_004668"
                     /gene_synonym="msbB"
     CDS             5231..6229
                     /gene="lpxM"
                     /locus_tag="ACHKW0_004668"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052689.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009276 - Gram-negative-bacterium-type
                     cell wall [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HFO7477344.1"
                     /translation="MVPPSAVLCYHNEISRQIPVNMKNIRTEFIPRFNLTLCFPRYWM
                     TWTGIGIICVFAMVPPALRDPLLGKLGMLVGRLGKSAHQRALINLSLCFPEYSDKEKE
                     NIVDAMFATASMAVVLMAELALSGPDKISHRIRWNGLEIVEKMAQNNEKVIFLVPHAW
                     GVDIPAMLMAASGRKMAAMFHNQRNPVVDYVWNSVRRRFGGKLHARNDGIASFVRSVR
                     QGYWGYYLPDQDHGPEFSEFADFFATYKATLPVIGRLSRISGARIIPLFPVYDGKTHH
                     LTIHVSPPLAIRQKSDAHIARQINEVVENFVRPHPEQYTWILKLLKTRKEGEEDPY"
BASE COUNT         1669 a         1368 c         1566 g         1778 t
ORIGIN      
        1 ccctaattca actaattgga ggcattacca tgcattagtg caagcgcctt gtggattctt
       61 ttgctaatga cggaaacata ttgtacaata ttgcatttat ccctcaataa ataaatgagt
      121 tatcagattg caaaaatctg ttatcaaact aattcaaata aaaagagatt gatacgacca
      181 acgtacacaa aaattttcga tattcgaact aaataacagc tcctaatacc gctacacttt
      241 agctatccag tctgcgcctc agaaatggac gatgccgttg aaggactggt gtatggcaat
      301 gagccgcttt attatcgaat tcggtgaccg cctggacggt cacttcggag aaaaggcatt
      361 tacacagcat cctaaacagg ttcagcatag gtgatatacc gccttttcat tttcttctcc
      421 attatttata agggaggtgt attattatgc aatattattg gttttaccca ttgttttttc
      481 agttcatgaa tgtttgcaaa aaaaataaat gaacaacaat tttatgtgtt taaaataaat
      541 taattttact tctgtaatgg tgtcatgatt agcagctttt taaactaata tcgactttca
      601 ataaataagg acaggtaatg gacaacaaaa atatctgaac ccgggtgaga ttcataagtt
      661 gcacagtgct gtctttaaag tgccacaccc ggaacgtaat cactgcctgc tccttatggg
      721 gtatcttcat ggtgtccaag catcagagtt attaggtata aaactgtctg atattgattt
      781 gcaggccggt aacctgaata tccggcggct ctaaacggtt tgtcaactca gcccctcggc
      841 tgaaacagga ggtacagtcc attcgtcgct ggctgaaact tcgggatgaa tgggtagaag
      901 agggatatga atggttattt aacctctatg gcaggtctgc tacaggtatt actgttgttc
      961 tggggggggg gcactggtgt ggttaatatc tacgatgcaa gagattctca cgatggcccg
     1021 gtccatcagt gcatgcggcg cgatattgtg cgagtatttt tcagggggag tggtatccgg
     1081 atgcagaaaa ttccgctact cgactggttt tatgcaataa atcagtatga taatcttctg
     1141 attttttact gaggcggata tctctcgctg tgtttactga aattgcggta ttctgttttt
     1201 tctgaaaaac agaaaatctg attaaaaaga gcatatgcgt taagggaaat ttaagttctg
     1261 gttgattaac tgataaaaat attaaaaaat ccctgttgtc ggtaatctcc ggaccattcc
     1321 gggattacac aataatatca ccatgtgtga tatctatgga tcctcactgt tgtttacgcg
     1381 aggatatttt cagaatgctt aacagacgat aattttttat gttgaatgca aggcatcttc
     1441 ccgtactgat gtatcatcat gtcagtcgtt gtccggggct tgtgacactc tcacctgaaa
     1501 cattttgtga acagatgaag tggctcgcag ataataactg gcggacggta acatctgctg
     1561 agcttgaata tttttatcag gggggaacgc ttccacgaaa gagtgtcatg ctcacattcg
     1621 atgatggtta tcttgataac tggctcagag catggccggt cctgaaggaa tataaccttc
     1681 atgcacatat ttttctcatt acggggctga ttggtaaggg aaacgtacgt agcaggcagg
     1741 agcaggaata ttctcaccgc gactgtgaac ggttgattgc cgataaccgt tcagatgagg
     1801 tcatgcttcg ctggtctgaa gtccgggaaa tgcgtgattc cggactggtt gaatttcatg
     1861 tgcacaccca ttcacataaa cgctgggaca ggttgtctgt atcacgtgct gaacagtgcc
     1921 ggctcatgaa agaagatatt ctggtgggaa aacaatgtct gacggaaaag ctggggttct
     1981 gtagttccca tctgtgctgg cctgaaggat attataatcg ggattatatt aatctcgccg
     2041 gtaagcttgg tttctcttat ttatatacaa ccgaaagaag aatgaactgt cctgaaaatg
     2101 gttcattacg catagggcgt atcagcacca aagagcggga acattccggc tggctgaaac
     2161 gacgcctttt ttattataca acgccacttt tttcttcagt actggctctt cataaggggc
     2221 caagattacc agataattaa tgatgaaaat tttattcact gaatcttcgt cagatattgg
     2281 gggccaggag ctgcaggcac tggcccagat gactgctctt caaaagcagg gacattccgt
     2341 tttacttgcc tgcagggaaa aaagtaaaat tgctccggaa gccagaaaaa gagggcatga
     2401 tgtcactttt atccctttca ggaacagcct tcaccttcct tccatcctca gacttcgacg
     2461 gattatcggg gaatttaaac ctgatctggt catctgccat agtggccatg acagtaatat
     2521 agcaggactg agccgtctga tatgctgtca ccgcttcagt attgtcagac aaaaaaccta
     2581 catcacccgt aagacccgaa cgttttccct gaattatctg tgtgatttca ttgttgtgcc
     2641 tagctcagcc atgatggcgc atctgatggc ggaaggtgtc aggacaccgg taacggttat
     2701 tccaccgggt ttcgactggc ccgcattgca taatgaagcc atgcgaccgt tacccctgca
     2761 tatccacgcc tgggctgcat cagcagacaa tgttccgctt atcgttcagg tggggatgct
     2821 acgtcccgaa aaaggacatg aatttatgct gcgtgtattg tatcagctga agatggaagg
     2881 gaaatcattt cgctggctcg tggtgggcgc cgggcgtgaa gaatacgaag cacgtctgcg
     2941 tcagcagaca gaacacctgg gaatgagcgg agatgtgtta atggcaggtg cccttttccc
     3001 agcgttaccg gtctaccgga ttgccagtgt ggtggtcatg ccttcggaaa atgaagcgtt
     3061 tggtatggta ctggctgaag cgtcagtctc tggcgtgccc gttatcgcca gtgagacggg
     3121 cgggatccct gatgtcattc agaagaatgt gacagggacg ctattacccg tgggggatgt
     3181 gtctgcatgg accggcgcac tgcgcgattt tttatcccga ccggaacgct tccggatgat
     3241 ggcagccagc gccagagaag atattgagtg ccggtttgat attaacagaa cggcacagat
     3301 cattgtgtct ctggctagtc aggctaaggg gaaatgtaac agataacggc ggatcctctt
     3361 ttcagcccga attttgttca gcggttttcc ggtaacggag taatttatca cattgtttaa
     3421 atacggggac ggtcaatgca tttaaatacc ggacagaaca ggcctacttt cagctggagt
     3481 gcgctgggat gggcaatttt ttattttggc tttttttcca ctctcctgca ggtcatcatc
     3541 ttcagcagtg ggtacagcgg aacgaatgga atacgggact cactgttatt cagttgtctg
     3601 tggttgatcc cggtgtttct ctatcctgat cggataaaaa taattgcagc tgttgtcggt
     3661 ttcattctct ggggcacgtc gctggcagca ctttgttatt attttctcta tggtcatgaa
     3721 ttctctcaaa gtgttctttt cgttatgttt gaaacgaatg ccagagaggc tggtgaatat
     3781 ttcagccagt atttcagcct taaactgttg cttatctcgc tggtatatac tgcggtgtcc
     3841 gtttttctgt ggacacgtct gcgtcctgta tatattcctt tgccatggcg gagaattgtc
     3901 tctttcctgc tgctttatgc tctgcttctg catccggttg ttctgaaatc gttaatcaga
     3961 caggagccgc tgaatgatac tctaggcaaa ctggcatccc gaatggagcc tgctgccccc
     4021 tggcagtttg tatccagtta ttatcagtac catcagcaac tgaatgcact gacaaccttc
     4081 ctgaatgaaa atagcgcact gccaccactg ggtaatctca gggatgaatc aggggagaga
     4141 ccacgcacac tggtcctggt gattggtgag tcgacacagc gcgaacgcat gagcttatac
     4201 gggtatctac gtgaaacgac gccggagctg gatgcactgc gtaaaaccga tccgggtctt
     4261 actgtgttta ataatgtggt ggcatcgcgt ccgtacacca ttgaagcatt gcaacaggcc
     4321 cttactttcg ccaacgaaaa gaatcctgat ctgtatctga cgcagccgtc gctgatgaac
     4381 atgatgaagc aggcaggcta taaaacattc tggattacca accagcagac aatcacagcc
     4441 cgtaacacca tgctcactgt attttcgcgc cagacggaca ggcagtacta catgaatcag
     4501 cagcgaacac aaagtgcgcg tgaatatgac actaacgtgc tgaagccgtt ccgggaagtg
     4561 ctgaatgacc ctgcaccaaa gaaactgatc atcgtgcatc tgctgggtac gcacattaag
     4621 tataaatacc gttatccgga aggtcagggg cggtttgatg gcattacagg gcatattccc
     4681 actggattaa atgcgaaaga gctggaagtg tataacgatt acgataatgc caatctgttt
     4741 aacgatcatg tggtggccag tctgataaag gacttcagag caactgcgcc ggacggcttt
     4801 ctgctttatt tttcagatca tggtgaagaa gtatatgata ctccgccata taaaacgcag
     4861 ggacggaatg aagataaccc cacacgtccc atgtacactg ttccgttcct gctgtggacc
     4921 tcggaaaagt ggcatgctgc acatccgcga gatttttcgc agtatgttga ccgcaaatac
     4981 agtctggctg aactgatcca cacctggtca gatttggcgg gactgacata cgatggttac
     5041 gatccgaccc gttctctggt gaatccgcaa ttcagggaaa ccccccgctg gattggaaat
     5101 ccgtataaaa agaatgggct cactgatttc gacactcttc cgtatggtga gccatagtat
     5161 tttcttatct tcggttccgt tctgaaggca ggaggatgtg ttttcctctg ctgtccggtg
     5221 gcctccatgg atggtaccac cttctgctgt actctgttat cataatgaga taagcaggca
     5281 gataccggta aatatgaaaa atatcagaac tgagtttatt ccccgcttta accttacatt
     5341 atgcttcccc cgttactgga tgacgtggac aggtatcggt attatctgtg tgtttgcaat
     5401 ggtccccccc gcactgagag atccattgtt gggaaaattg ggcatgctgg ttgggcgtct
     5461 ggggaaaagc gcacatcaac gggccttgat taatttatcg ctttgttttc cggagtacag
     5521 tgataaagaa aaggagaata tcgttgatgc catgtttgca acagcctcca tggccgttgt
     5581 gcttatggct gaattagcac tgtccgggcc tgataaaatt tcgcatcgta tacgctggaa
     5641 tggtctggag attgtggaga agatggcaca gaacaatgaa aaggtcattt ttcttgtgcc
     5701 acatgcctgg ggagtggata ttcccgcaat gctgatggct gcgtcgggca gaaaaatggc
     5761 agcgatgttt cataaccaga gaaatccggt ggtggattat gtctggaaca gtgttcgtcg
     5821 tcgtttcgga ggaaaactgc acgcacgtaa tgacggtatt gcctcctttg tccgctcagt
     5881 ccggcaggga tactggggat attatcttcc tgatcaggat catggcccgg aattcagtga
     5941 gtttgcagat ttttttgcga cgtataaagc aactttaccg gttatcggac ggctcagcag
     6001 gatcagtggt gcacggataa taccgctttt ccctgtttat gacggaaaga cccatcatct
     6061 gactattcat gtcagcccgc cacttgctat acgacagaag agtgatgctc atattgcgcg
     6121 acagataaac gaagtggttg aaaatttcgt tcgtccacat cctgagcagt atacgtggat
     6181 cctcaagttg ctgaaaacgc gtaaagaagg ggaagaggat ccttactgag gcaggttttt
     6241 caggataatg agcttaccca gcaatagtgg acacacgcta agtgagtaaa ctctcagtca
     6301 gaggtggctc atatgacaaa agtaagcgta cagcctgaac cgtctggtca gaatctgacg
     6361 aattagacaa agtggtgtcc a
//
LOCUS       DAZFXT010000203         6261 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.203, whole genome shotgun sequence.
ACCESSION   DAZFXT010000203 DAZFXT010000000
VERSION     DAZFXT010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.203"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(211..2244)
                     /gene="betT"
                     /locus_tag="ACHKW0_004669"
     CDS             complement(211..2244)
                     /gene="betT"
                     /locus_tag="ACHKW0_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HFO7477345.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALIALSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
     gene            2373..2960
                     /gene="betI"
                     /locus_tag="ACHKW0_004670"
     CDS             2373..2960
                     /gene="betI"
                     /locus_tag="ACHKW0_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HFO7477346.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVAGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPREQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            2974..4446
                     /gene="betB"
                     /locus_tag="ACHKW0_004671"
     CDS             2974..4446
                     /gene="betB"
                     /locus_tag="ACHKW0_004671"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008802 - betaine-aldehyde dehydrogenase
                     (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HFO7477347.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETLFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCRDEMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLSAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            4460..6148
                     /gene="betA"
                     /locus_tag="ACHKW0_004672"
     CDS             4460..6148
                     /gene="betA"
                     /locus_tag="ACHKW0_004672"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HFO7477348.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKKMSRDVN"
BASE COUNT         1542 a         1726 c         1715 g         1278 t
ORIGIN      
        1 tattaataaa tatataacac tataggactg aatccattca gctaatacaa aataaaatcg
       61 ttaattaata aaatataaaa tcatgaagtg atacaagagg aattgcagga tatgacatga
      121 caatcatata taatatatgc ataatttttt acagcgagtc aatgattccc gcgccatatg
      181 cagacatggc gcggttttat gcaaaaacaa tcacgcgtcc gggaacatca cgctatggcc
      241 cggcgcttca cgatggagat gaataaagtt aaggtgccgc tcgtactggt caagaatatc
      301 ggtgatcacc tgctctttgc tgtagtccat caggtcgttg ccctggctgc cttctaacag
      361 gaaggtttcc agccggtagt aggtcgattt accgctacgt gcgcggtagg taaagcccgg
      421 caccgaatat tgctgcggcc aaatctgata gacaaagttt tgttcttcgc ccatatgcac
      481 caacaaatcc agatgcccca actgttgtcc ctcttccggc ggcaggcttt ttagctccac
      541 gtatgcgccg cgcaaccgca gctcctgcgc cacttcttcc attgccgggt aacagaccgt
      601 ctccatcatc tgtttagtgt aacgcgtgcc cggataattc atcaggcgcg agagacgttt
      661 tttccagctc aggcgatcct gaagccccag cggtcgcggt gcggtatcgc ggttggcact
      721 ttcacggcgg taatcttcta ccttcagaga tttatacaac cccgccatca cgaagaagat
      781 cacaaagctg aacggcagcc ccataatcac cgtggtgttt tgcagcgcgg atatcccgtt
      841 ggtcatcagc atgccgagcg tcagcaggcc aatcgccacc gaccagaaga cgcgcagcca
      901 gccgggggcg tcgctgttga tatctttaag ctgcgaggtg aaattcccca gcaccagcgc
      961 accggagtcc gccgaggtca cataaaacag caggccagta atggtggcga cggaggcgct
     1021 aaaggtaaac gccggatact gcgccagcag gctgtaaaag ccgcgctccg gatggaccat
     1081 cgcttcctcg gcaaatgccg cgccgccgtg gatgatttca tacagcgcgc tattgccgaa
     1141 caccgagagc cataacagcg tgaaggtaaa cggaataatc aacgtgccca gcacgaactg
     1201 gcgaatggta cgcccacgcg agatacgcgc caggaacaag ccgacaaacg gcgaccatgc
     1261 cacccaccat gcccagaaga agagcgtcca gttattcatc cactcaactg gacggtcgaa
     1321 ggcaaaactg ttgagcgtca tgcccataaa gcgattcaca tagtcgccaa cattcagcac
     1381 cagcgcatta agcaggaacg aggtgtcgcc cataaacaat acgaacagga tcaatcccag
     1441 cgccagcgca acgttaagtt ccgataacac acgaatcccc ttatcgacac cggaggtgac
     1501 agagatcgtg gcgattatca ccgacaaggc gatcagtgcc gcttttgccg ccatcgaatc
     1561 gggaatatca aacagtacgc tcaagccata gttaagctgc accacaccga taccgagcgt
     1621 agtggcaata ccgaagatgg tgccgatcac cgctgcaata tccactgagt gccctatcgg
     1681 cccgttaatc cgtttaccga agatcgggta cagcgccgag cggatggtga gcggcaaatt
     1741 ataacgatag ctaaagtatc cgagcgccat gcccatcagc gcatacatcg accagccggt
     1801 taagccgtag tgaaacagcg tccagaccat cgcctgacgc gcggcctcaa tcgtctgtcc
     1861 cgcgccttcc ggcggctgca tatactgcgt taccggttcg gctacggaga agaacatcag
     1921 gtcgataccg atcccggcag caaacagcat cgccgcccaa ctcagcaggc tgaattccgg
     1981 tttggattgt tctggcccga gcttcaccga accaaaacgc gaacaagcga tacagaccac
     2041 aaagacaata tagagcgttg ccgccagtag atagtaccaa ccgaaggttt tagaaaccca
     2101 gtccagcgtg cggccaatcc acagggccga gaagtcgcga aacaggatcg ttgtcaggga
     2161 aaacaacaaa atcagtccgg cggaggtgta aaacaccacc ggattgattt tgtccttttc
     2221 cctgctgtgt gaaaggtctg tcatccagta tccccactgt tattgttacc atttaaaatc
     2281 aaattcgtaa caattaagac acattttata ttgaacgtcc aatcaataac cgctttaata
     2341 gataaacaac gctgatgaat ggagtggcga aaatgcccaa attggggatg cagtcgatcc
     2401 ggcgcagaca actgatcgac gccacactgg aagcaataaa tgaagtgggc atgcacgatg
     2461 caacgatcgc gcagatcgcc cgccgtgcag gcgtttctac ggggatcatc agccactatt
     2521 tcagggacaa aaatggtctg ctggaagcaa ccatgcgcga catcaccagt cagttacgtg
     2581 acgcggtttt gaatcgatta catgcacttc cgcagggcag tgcagagcag cgattacagg
     2641 cgattgttgc cggaaacttc gatgaaactc aggtgagcag tgcggcgatg aaagcctggc
     2701 tggcgttctg ggccagcagt atgcatcagc cgatgctcta tcgtttacag caggtcagca
     2761 gccgccgctt gctgtcgaat ctggtgagcg agtttcgccg cgaattgccg cgcgaacagg
     2821 cacaggaagc gggctacggc cttgccgcgc tgattgacgg attatggctg cgcgcggcac
     2881 tgagcggcaa accgctggat aaacctctcg cacactcgct gacccgtcac tttattactc
     2941 agcatttacc caccgattaa ccgaggagac gtgatgtccc gaatggcaga acagcagctt
     3001 tatatacatg gtggttatac ctccgccacc agcggtcgca ccttcgagac cattaacccg
     3061 gccaacggta acgtgctggc gaccgtgcag gccgccgggc gcgaggatgt cgatcgcgcc
     3121 gtgaagagtg cccaacaggg gcaaaaaatc tgggcggcga tgaccgctat ggaacgctcg
     3181 cgtattctgc gtcgggccgt tgatattctg cgtgaacgca atgacgaact cgcaaaactt
     3241 gaaaccctcg acaccggaaa agcatattcg gaaacctcaa ccgtcgatat cgttaccggt
     3301 gcggacgtgc tggagtacta cgccgggctg atcccggctc tggaaggcag ccagatcccg
     3361 ttgcgtgaga cgttatttgt ttatacccgc cgcgaacctc tgggtgtggt ggcggggatt
     3421 ggcgcatgga actacccgat tcagattgcc ctgtggaaat ccgccccggc gctggcggca
     3481 ggcaacgcaa tgattttcaa accgagcgaa gtcaccccgc ttaccgcgtt aaagctggct
     3541 gaaatttaca gcgaagcggg cctgccggac ggcgtattta acgtgttgcc gggggtgggc
     3601 gcggagaccg ggcaatatct gaccgagcat ccgggcattg ccaaagtgtc atttaccggc
     3661 ggtgtcgcca gcggcaaaaa agtgatggct aactcggcgg cctcttccct gaaagaagtg
     3721 accatggaac tgggcggtaa atcaccgctg atcgttttcg atgatgcgga tctcgatctc
     3781 gccgccgata tcgccatgat ggcaaacttc ttcagctccg gtcaggtgtg taccaatggc
     3841 acccgcgtct tcgttccggc gaaatgcaaa gccgcatttg agcagaaaat tctggcgcgc
     3901 gttgagcgca ttcgcgcggg cgacgttttc gatccgcaaa ctaacttcgg cccgctggtc
     3961 agcttcccgc atcgcgataa cgtgctgcgc tatatcgcca aaggcaaaga ggaaggcgcg
     4021 cgcgtactgt gcggcggcga tgtactgaaa ggcgatggct tcgataacgg cgcatgggtt
     4081 gcaccgaccg tgttcaccga ttgccgcgac gaaatgacca tcgtgcgcga agagatcttc
     4141 ggtccggtga tgtcgcttct cacttatgaa tctgaggacg aagtgattcg ccgcgccaat
     4201 gataccgact acggcctgtc ggcgggcatc gtgacggcgg acctgaaccg cgcgcatcgc
     4261 gtcattcatc agctggaagc gggtatttgc tggatcaaca cctggggcga atccccggca
     4321 gagatgcccg ttggcggcta caaacactcc ggcattggtc gcgagaacgg cgtgatgacg
     4381 ctccagagtt acacccaggt gaagtccatc caggttgaga tggctaaatt ccagtccata
     4441 ttctaaccag gaggtttatt tgcaatttga ctacatcatt attggtgccg gctcagccgg
     4501 caacgttctc gctacccgtc tgactgaaga tccgaatacc tccgtgctgc tgcttgaagc
     4561 gggcggcccg gactatcgct ttgacttccg cacccagatg cccgcagcgc tggcgttccc
     4621 actacagggc aaacgctaca actgggccta tgaaacagag cctgaaccgt ttatgaataa
     4681 ccgccgcatg gagtgcggac gcggtaaagg cctgggtgga tcgtcgctga tcaacggcat
     4741 gtgctacatc cgtggcaatg cgctggatct cgataactgg gcgcaagaac ccggtctgga
     4801 gaactggagt tatctcgact gcctgcccta ctaccgcaag gccgagactc gcgatgtggg
     4861 cgagaacgac taccacggtg gcgatggccc ggtgagcgtc accacctcca aacccggcgt
     4921 caatccgctg tttgaagcga tgattgaagc gggcgtgcag gcaggctacc cgcgcacgga
     4981 cgatctcaac ggctatcagc aggaaggttt tggcccgatg gatcgcaccg tcacgccgca
     5041 gggccgtcgc gccagtaccg cgcgcggtta tctcgatcag gccaaatcgc gccctaacct
     5101 gaccattcgt actcacgcta tgaccgatca catcattttt gacggcaaac gcgcggtggg
     5161 cgtcgaatgg ctggaaggcg acagcaccat cccaacccgc gcaacggcca acaaagaagt
     5221 gctgttatgt gcaggcgcga ttgcctcacc gcagatcctg caacgctccg gcgtcggcaa
     5281 cgctgaactg ctggcggagt ttgatattcc gctggtgcat gaattacccg gcgtcggcga
     5341 aaatcttcag gatcatctgg agatgtatct gcaatatgag tgcaaagaac cggtttccct
     5401 ctaccctgcc ctgcagtggt ggaaccaacc gaaaatcggt gcggagtggc tgtttggcgg
     5461 cactggcgtt ggtgccagca accactttga agcaggtgga tttattcgca gccgtgagga
     5521 atttgcgtgg ccgaatattc agtaccattt cctgccagta gcgattaact ataacggctc
     5581 gaatgcagtg aaagagcacg gtttccagtg ccacgtcggc tcaatgcgct cgccaagccg
     5641 tgggcatgtg cggattaaat cccgcgaccc gcaccagcat ccggcgattc tgtttaacta
     5701 catgtcgcac gagcaggact ggcaggagtt ccgcgacgca attcgcatca cccgcgagat
     5761 catgcatcaa cccgcgctgg atcagtatcg tggccgcgaa atcagccccg gcacggaatg
     5821 tcagacggat gaacagctcg atgagttcgt gcgtaaccac gccgaaaccg ccttccatcc
     5881 gtgcggtacc tgcaaaatgg gttacgacga gatgtccgtg gttgacggcg aaggccgcgt
     5941 acacgggtta gaaggcctgc gtgtggtgga tgcgtcgatt atgccgcaga ttatcaccgg
     6001 gaatttgaac gccacgacaa ttatgattgg cgagaaaata gcggatatga ttcgtggaca
     6061 ggaagcgctg ccgaggagca cggcgggata ttttgtggca aatgggatgc cggtgagagc
     6121 gaaaaaaatg agtcgtgatg tgaactgact caagaaccga tcagattgag agttaccgtt
     6181 ccaggcatgg ggacagaatc gttatataaa acataggggt aactctcatg cttcatatcc
     6241 agtattcatg atgctggctt t
//
LOCUS       DAZFXT010000204         6174 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.204, whole genome shotgun sequence.
ACCESSION   DAZFXT010000204 DAZFXT010000000
VERSION     DAZFXT010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.204"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            80..856
                     /gene="exuR"
                     /locus_tag="ACHKW0_004673"
     CDS             80..856
                     /gene="exuR"
                     /locus_tag="ACHKW0_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HFO7477349.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1096..1179
                     /gene="yqiM"
                     /locus_tag="ACHKW0_004674"
     CDS             1096..1179
                     /gene="yqiM"
                     /locus_tag="ACHKW0_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YqiM"
                     /protein_id="HFO7477350.1"
                     /translation="MLMYQTRRTYQNSNNIAVVHLLKPAWR"
     gene            1201..1863
                     /gene="yqjA"
                     /locus_tag="ACHKW0_004675"
     CDS             1201..1863
                     /gene="yqjA"
                     /locus_tag="ACHKW0_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family general envelope maintenance protein
                     YqjA"
                     /protein_id="HFO7477351.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1867..2250
                     /gene="mzrA"
                     /locus_tag="ACHKW0_004676"
     CDS             1867..2250
                     /gene="mzrA"
                     /locus_tag="ACHKW0_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HFO7477352.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2397..2765
                     /gene="yqjC"
                     /locus_tag="ACHKW0_004677"
     CDS             2397..2765
                     /gene="yqjC"
                     /locus_tag="ACHKW0_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 family protein YqjC"
                     /protein_id="HFO7477353.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2803..3108
                     /gene="yqjD"
                     /locus_tag="ACHKW0_004678"
     CDS             2803..3108
                     /gene="yqjD"
                     /locus_tag="ACHKW0_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqjD family protein"
                     /protein_id="HFO7477354.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3111..3515
                     /gene="yqjE"
                     /locus_tag="ACHKW0_004679"
     CDS             3111..3515
                     /gene="yqjE"
                     /locus_tag="ACHKW0_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HFO7477355.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3505..3804
                     /gene="yqjK"
                     /locus_tag="ACHKW0_004680"
     CDS             3505..3804
                     /gene="yqjK"
                     /locus_tag="ACHKW0_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HFO7477356.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3990..4382
                     /gene="yqjF"
                     /locus_tag="ACHKW0_004681"
     CDS             3990..4382
                     /gene="yqjF"
                     /locus_tag="ACHKW0_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HFO7477357.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4452..5438
                     /gene="yqjG"
                     /locus_tag="ACHKW0_004682"
     CDS             4452..5438
                     /gene="yqjG"
                     /locus_tag="ACHKW0_004682"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HFO7477358.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5731..6096
                     /gene="yhaH"
                     /locus_tag="ACHKW0_004683"
     CDS             5731..6096
                     /gene="yhaH"
                     /locus_tag="ACHKW0_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HFO7477359.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1546 a         1518 c         1553 g         1557 t
ORIGIN      
        1 aatttatata tgaccaatgg catacccatg aataaagtgg tatgataact tcgtccgcat
       61 tccactccgg gagacgcata tggaaatcac tgaaccacgc cgtttgtatc aacaacttgc
      121 cgctgacctg aaagagcgca tcgaacaggg cgtctatctg gtaggtgata aactgcctgc
      181 agaacgcttt attgccgatg aaaagaacgt cagccgtacg gtagttcgtg aagccatcat
      241 catgctggaa gttgaaggct atgtcgaagt gcgtaaaggt tcaggtattc atgtggtttc
      301 caaccagccg cgccatcagc aggcggctga caataatatg gaattcgcca attacggtcc
      361 gtttgagttg cttcaggctc gccagctcat cgaaagtaat attgccgagt tcgcggcaac
      421 tcaggtaacg aaacaagaca tcatgaaact gatggccatt caggaacagg cgcgcggcga
      481 acaatgcttc cgtgattccg agtgggattt acagttccac attcaggtag ccctggcgac
      541 gcagaactcc gccctggcgg ccatcgttga aaaaatgtgg acccaacgta gccataaccc
      601 gtactggaaa aaactgcacg aacacattga ttcccgtacc gtcgataact ggtgtgatga
      661 ccacgatcaa atcctcaagg cgctgattcg caaagatcct catgctgcta agctggcaat
      721 gtggcagcac ctggaaaaca ccaagatcat gttattcaac gaaaccagcg acgacttcga
      781 gttcaatgcc gaccgctatc tgttcgccga aaacccggta gtgcatctcg atacggccac
      841 cagtggcagt aaatgaaact cctgtccgac aggcgctgcg acgcgcctgt ttgtctcgtc
      901 ggtaagcgaa aagtataaag tgtcagcctg tgtaaatcct ctcgccaccc tcccctgcat
      961 tcagcaaaat cagactccac ggacatgtaa ttttgataac gaacaacgtt gacctttgtt
     1021 acaattagat tcaatttgaa tttatgtttt tgaatgcttt cttatctcac gatttaacag
     1081 ggaatagttc aggctgtgtt gatgtatcaa acccgcagaa cataccaaaa cagcaataac
     1141 attgcggtag tgcatctttt aaaaccagcg tggcgttaac cgattcacca ggaataatga
     1201 atggaacttt tgacccaatt gctgcaagcc ctgtgggcgc aggattttga aaccctggcc
     1261 aatccatcga tgattggcat gttgtatttt gtcttgtttg taattttgtt ccttgaaaac
     1321 ggcttgcttc cagcggcctt tttaccgggc gacagtttac tggtattggt cggcgtgttg
     1381 attgcgaaag gcgcgatggg ttacccgcaa acgattctgc tgctgaccgt tgccgccagc
     1441 ctcggctgct gggtcagcta tattcagggg cgatggctgg gcaatacccg caccgtacaa
     1501 aactggctat ctcatttacc cgcgcattat catcaacgcg cacaccatct ttttcataaa
     1561 cacggtttat cggcgctgtt aattggtcgc tttattgcgt ttgtcagaac actgctgccg
     1621 acgattgccg ggttatcagg gctgaataac gcgcgctttc agtttttcaa ctggatgagc
     1681 ggtctgctgt gggtattgat cctgacaact ctgggttaca tgctcggcaa aacgccggta
     1741 tttttaaagt acgaggacca gctgatgtca tgcctgatgc tgctcccggt ggtgctgctg
     1801 gtgtttggcc tggcaggttc tctggtcgtg ttatggaaaa agaaatatgg aaatcggggg
     1861 taagggatgc aaatacctcg catgtcgctt cgccagctag cctggtccgg cactgtttta
     1921 cttctggtcg gcacgctgtt gctggcctgg tccgcggttc gccagcaaga gtctacgctg
     1981 gcgattcgtg ccgttcatca aggcacaacg atgccagacg gtttttcgat ctggcatcac
     2041 cttgacgctc atggcattcc tttcaaaagt atcaccccca aaaacgacac tctgttaatt
     2101 acatttgact ccagcgacca gagcgccgcc gcaaaagcgg tcctcgacag aacattgccc
     2161 catggctaca tcattgcgca gcaggacaat aacagtcagg ctatgcagtg gctgacccgg
     2221 ttacgggata actctcatcg cttcggataa cttcctgtta tccgaaacat atcactcact
     2281 ttggtgattt caccgtaact gtctatgatt aatgaggcgg tggttaacac taccgcttcg
     2341 ttgtctggac aatcgttcct ttgtaatagg tccgaatcac aatggaaggt tcaagaatga
     2401 aataccgcat cgctttagct gtttctctct ttgctcttag tgccggtagt tatgccacta
     2461 ccctgtgtca ggaaaaggag caaaatatcc ttaaggagat cagctatgcc gaaaaacacc
     2521 aaaaccagaa tcgtattgac ggtctgaata aagccctgag tgaagtccgg gccaactgtt
     2581 cagatagcca gctgcgtgcc gatcatcaca agaaaatcgc aaagcagaaa gatgaggtgg
     2641 cggaacgcca gcaagattta gccgaggcga agcaaaaagg cgatgccgat aagattgcca
     2701 aacgcgaacg gaaactggca gaagcgcagg aagagctgaa aaagctggaa gcgcgcgact
     2761 actaactcac aatagtcact acttactcac ctggagaaaa ctatgtcgaa agaacacact
     2821 acggaacatc tgcgtgctga gttgaaatcc ctttccgata cgctggaaga ggtgcttagc
     2881 tcatctggcg agaagtcgaa agaagagttg agtaagattc gtagcaaagc ggagcaggca
     2941 ctgaaacaga gccgttatcg cctgggtgaa accggtgatg ccatcgccaa acaaacccgt
     3001 gtcgcggcgg cgcgtgctga tgagtatgtg cgtgaaaatc cgtggaccgg cgtgggaatt
     3061 ggcgctgcaa tcggtgtagt gctcggcgtt ctgctgtcgc gtcgttaatt atggcggaca
     3121 ctcatcacgc gcaagggccc ggtaaaagcg ttctgggcat cgggcagcga attgtttcca
     3181 tcatggttga aatggtggaa acacgtctgc ggctggcggt ggtggagctg gaagaggaaa
     3241 aagctaatct ctttcaactt ttactgatgc tgggcctgac gatgcttttc gctgcatttg
     3301 gtcttatgag cctgatggtg ctaattattt gggcggttga tccgcaatat cgcctgaatg
     3361 cgatgattgc caccaccgtg gtgttgctgc tactggcact gattggcggt atctggacgt
     3421 tacgtaaatc gcgtaaatcc accttgctgc gccatacacg ccatgagctg gcaaacgatc
     3481 ggcaactgct cgaggaggag tcccgtgagc agtaaagtcg aacgtgaacg acgtaaggcg
     3541 caactgctta gccagatcca gcaacaacgg ctggatcttt ccgccagtcg tcgtgaatgg
     3601 ctggaggcaa caggcgctta cgatcgtcgc tggaatatgc tgctaagtct gcgctcctgg
     3661 gcgctggttg gcagtagcgt gatggcgatc tggacgattc gccatcctaa tatgctggtc
     3721 cgctgggcca gacgcggttt tggcgtatgg agcgcctggc gtctggtaaa aacgaccctc
     3781 aagcagcaac agcttcgcgg ttaacttccc tctggccgga gcaattccgg ccttatccct
     3841 caaatttttt gaagattttt gacagttttc cttgctaaca atcatcattc accacgttta
     3901 tgattctctc catcgacagc aacgacgcta ataccgcgcc cttgcacaaa aaaacaatca
     3961 gcagcctgag tggcgcagtg gagagtatga tgaaaaaatt agaagatgtt ggtgtactgg
     4021 tagcgcgcat tttaatgccg attctgttta ttaccgcagg ctggggaaaa atcaccgctt
     4081 actctggtac ccaacaatat atggaagcaa tgggtgtccc gggttttatg ctgccgctgg
     4141 tgattctgct tgagttcggt ggtggtctgg caatcctgtt cggtttcctg actcgcacca
     4201 cagccctgtt tactgcgggc tttacgctgc tgacggcatt tttatttcac agcaactttg
     4261 ctgaaggcgt caactcgctg atgttcatga aaaacctgac aatttctggc ggattcctgc
     4321 tgctggcaat taccggtccg ggcgcgtata gcatcgaccg cctgctgaat aaaaagtggt
     4381 aagcacgcgc tatacttaac gataaaaaac gaggaggaag ctcctcgttt ttgctattgg
     4441 aggagagaaa aatgggtcaa ctgattgacg gcgtctggca tgacacctgg tacgatacca
     4501 aatctaccgg cggtaaattt caacgttcag cttccgcatt tcgtaactgg ctcactgccg
     4561 atggcgcacc tggccccact ggcacaggcg gttttatcgc agagaaagat cgttatcatc
     4621 tctatgtttc actcgcctgc ccgtgggcgc accgcacgct gatcatgcgc aaactcaaag
     4681 gactggaacc gtttatttcc gtttccgtag tgaacccgct gatgctggaa aacggctgga
     4741 cctttgatga cagttttccg ggagcaaccg gagatacgct ctatcaacat gaatttctgt
     4801 atcagcttta tctccacgcc gatccacact acagcggacg agttactgtt cccgtgctgt
     4861 gggacaaaaa gaaccacacc atcgtcagca acgaatcagc agaaatcatc cgcatgttta
     4921 ataccgcgtt tgatgcgctg ggcgcgaaag cgggtgatta ctacccacca gccctgcaaa
     4981 cgaaaattga cgaacttaac ggctggattt atgacaccgt taacaacggc gtgtataaag
     5041 ctggttttgc caccagccag caagcctacg acgaggcggt ggcgaaagtg tttgaatcgc
     5101 tggcgcgact ggagcagatt ttaggccagc atcgttacct gaccggcaac cagctaaccg
     5161 aagccgatat tcgcctgtgg accacgctgg tccgttttga tccagtgtat gtgacccact
     5221 tcaagtgtga taagcaccgc atcagcgatt accttaatct gtatggcttc ctgcgtgata
     5281 tctaccagat gccgggaatt gccgaaacag tcaatttcga tcacatccgt aatcattact
     5341 tccgcagcca taagaccatc aaccctacgg ggattatttc aattggtcca tggcaggatc
     5401 tcgatgaacc gcatggacga gatgttcgct tcggttaaaa aaaggcacct tacgaggtgc
     5461 ctctgataat tagctaagtg catgttgttc atgtcggatg cggcgtgaac gccttatcaa
     5521 acctacaaaa catttatatt caatatgttg caatgaccgc gcaggccaga taagcgtagc
     5581 gcatctggcg attttgcgct tgtcatcagt cttagcgcgc cttcacagca ctttattaat
     5641 tctttgaacg aatatttact gccatttcat tcacgtctat tcttaatttg ctgctttaaa
     5701 aacaagtgat tgagcaaaat tgaggcaaaa atggactggt atctgaaagt actaaaaaat
     5761 tatgtcggtt tccggggtcg tgcgcggcgc aaagagtact ggatgtttat tctggtcaac
     5821 atcatcttta cgttcgtgct ggggctgctg gataaaatgt taggctggca acgcgctggt
     5881 ggcgaaggca tcctgacgac aatctacggt attctggtgt ttttaccgtg gtgggcagtt
     5941 cagttccgcc gcctgcacga caccgaccgc tcggcgtggt gggcactgtt gttcttaatc
     6001 ccgtttatcg gctggcttat tatcatcgtc tttaactgcc aggcgggtac gccaggcgaa
     6061 aaccgctttg gaccagatcc gaaactggaa ccttaaaaca aatctgctcg ccggatgtct
     6121 gactcatccg gcttttatca tcatgttgat gaatgaataa tttactaatg ttta
//
LOCUS       DAZFXT010000205         6045 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.205, whole genome shotgun sequence.
ACCESSION   DAZFXT010000205 DAZFXT010000000
VERSION     DAZFXT010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6045
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.205"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            148..1281
                     /gene="ompN"
                     /locus_tag="ACHKW0_004684"
     CDS             148..1281
                     /gene="ompN"
                     /locus_tag="ACHKW0_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HFO7477360.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAAGGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1422..1856
                     /gene="uspF"
                     /locus_tag="ACHKW0_004685"
     CDS             1422..1856
                     /gene="uspF"
                     /locus_tag="ACHKW0_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HFO7477361.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            2032..2967
                     /gene="ttcA"
                     /locus_tag="ACHKW0_004686"
     CDS             2032..2967
                     /gene="ttcA"
                     /locus_tag="ACHKW0_004686"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HFO7477362.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(3096..4469)
                     /gene="dbpA"
                     /locus_tag="ACHKW0_004687"
     CDS             complement(3096..4469)
                     /gene="dbpA"
                     /locus_tag="ACHKW0_004687"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HFO7477363.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4499..4672)
                     /gene="ynaL"
                     /locus_tag="ACHKW0_004688"
     CDS             complement(4499..4672)
                     /gene="ynaL"
                     /locus_tag="ACHKW0_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaL"
                     /protein_id="HFO7477364.1"
                     /translation="MTTLIYLQIPVPEPIPGDPVPVPDPIPRPQPMPDPPPDEEPIKL
                     SHRERRSARIRAC"
     gene            complement(4947..5930)
                     /gene="zntB"
                     /locus_tag="ACHKW0_004689"
     CDS             complement(4947..5930)
                     /gene="zntB"
                     /locus_tag="ACHKW0_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005385 - zinc ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071577 - zinc ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HFO7477365.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHESAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            complement(5944..5991)
                     /gene="ydaN"
                     /locus_tag="ACHKW0_004690"
     CDS             complement(5944..5991)
                     /gene="ydaN"
                     /locus_tag="ACHKW0_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YnaN"
                     /protein_id="HFO7477366.1"
                     /translation="MLRNRARRLDYKIMR"
BASE COUNT         1603 a         1510 c         1426 g         1506 t
ORIGIN      
        1 ttatcatttc agaggaatta tttgattaag gttttactta aggcgtaaca aatgatattt
       61 atcggctaac tgaacttctc ctttaggatg ttttcactcc ccgaaatggg gataaaaggc
      121 aaataaaata actaaaggat ttattcaatg aaaagcaaag tactggcact tttaattcct
      181 gccctgctcg ccgcaggtgc tgcacatgca gccgaagttt ataataaaga cggcaacaaa
      241 ttagatctgt atggcaaagt tgatggcctg cattattttt ctgataattc ggcgaaagat
      301 ggcgaccaga gctatgcgcg tctgggtttt aaaggcgaaa cccaaattaa cgatcaactc
      361 actggctacg gtcaatggga atacaatatt caggcaaaca acactgaatc ttcaaaaaac
      421 cagtcatgga cccgtctggc atttgccggg ctgaaatttg cagattacgg ttctttcgat
      481 tacggacgta attatggcgt aatgtacgac atcgaaggct ggaccgatat gctgcctgaa
      541 tttggcggtg actcttatac caatgcagac aactttatga ctggtcgagc caatggcgtc
      601 gcgacttatc gtaatactga tttcttcggt ctggtaaatg gtctgaactt cgcggtgcag
      661 tatcaaggta acaacgaagg tgccagtaat ggtcaggaag gcaccaacaa cggacgtgat
      721 gttcgccatg aaaacggtga cggctggggt ctttccacaa catatgattt aggcatgggc
      781 tttagcgctg gtgcggcata cacctcttct gaccgcacca atgatcaggt taaccatact
      841 gcggcgggtg gtgataaagc agacgcgtgg actgctgggc taaaatacga tgctaacaat
      901 atttacctgg caaccatgta ttcagaaacg cgtaatatga ccccgtttgg cgacagcgat
      961 tatgctgttg caaacaaaac ccagaacttt gaagtcactg cacagtacca gtttgatttt
     1021 ggtctgcgtc cggcagtctc tttcctgatg tctaaaggcc gtgacctgca cgctgccggt
     1081 ggtgcagaca acccggcagg tgttgatgat aaagatctgg ttaaatacgc cgatgttggc
     1141 gcgacttact atttcaataa aaacatgtcc acttatgttg actataaaat caacctgttg
     1201 gatgaagatg acagtttcta cgctgccaat ggcatctcta ccgatgatat tgtcgcttta
     1261 ggtctggttt atcagttcta aatcctcctg cccgctgtta tggcgggctt tttctgctta
     1321 ttcttcctcc tttgatctaa attaaaaatg tgaactccgt cattacacaa aaagtgtcat
     1381 ctggcgttac actttatgcg gatactaaaa caggaggttt tatgaacaga acgattcttg
     1441 tccctatcga tatttccgat tcagaattaa ctcaacgcgt gattagccac gttgaggcag
     1501 aggcaaagat tgatgatgca gaggttcatt tcctgacggt aataccttca ctgccctact
     1561 atgcctctct gggtttagcg tattccgcag aattaccggc aatggatgac ctgaaagcgg
     1621 aagccaaatc gcaactggaa gagatcatta aaaaatttaa actgccaacc gacagagtgc
     1681 atgtccatgt tgaggaaggc tcgcccaaag accgcattct ggaaatggcg aagaagatcc
     1741 ccgctcatat gatcatcatt gcttcccatc gaccggatat caccacttat ctgctcggtt
     1801 ccaacgccgc agctgtagtg cgtcacgcag agtgctccgt gctggttgtg cgctgacact
     1861 aacgcccgca cattgctgcg ggctttttga ttcatttcgc aaatgtgctg acattttccc
     1921 ctctaatccg taccatacac gccactgttt ttatatcaga tttcttatgc tgggcattcc
     1981 ggcatgacgc atactcttct gatgccatat aacgaattga gtcgctttta aatgtcgcaa
     2041 aatcaagaaa ttagtaagaa agaacaatac aacctgaaca aattacaaaa acgtctgcgt
     2101 cgtaacgtgg gcgaagccat tgctgacttc aatatgattg aagaaggcga tcgcatcatg
     2161 gtttgcctct ccgggggtaa agacagctat accatgctgg agattctgcg caatttgcag
     2221 caaagcgcgc caatcaattt ttcgctggtg gctgttaacc tcgatcaaaa gcaaccgggc
     2281 ttcccggaac acgttctgcc cgagtatctt gaaaagctgg gcgttgagta caagattgtt
     2341 gaagagaata cttacggtat cgtgaaagag aagattccag agggcaaaac cacttgctca
     2401 ctgtgttctc gccttcgtcg cggtatcctt tatcgtaccg caacggaact gggggcgacg
     2461 aagatcgcgt tgggtcacca tcgtgacgat atcctgcaaa cgttgttctt aaatatgttc
     2521 tacggcggca agatgaaagg tatgcctccg aaactgatga gcgatgatgg caaacatatc
     2581 gttattcgtc cgctggccta ctgccgcgag aaagatattc agcgatttgc cgatgcaaaa
     2641 gcgttcccga ttattccgtg caacctgtgc ggttcacagc ctaacctgca acgtcaggtg
     2701 attgctgaca tgttgcgtga ctgggataaa cgttatccag ggcgtatcga gacgatgttc
     2761 agcgcgatgc agaatgtggt gccgtcgcat ctgtgcgata ccaacctgtt cgattttaaa
     2821 ggcattaccc acggttctga agtggttaac gggggtgatc tggcgtttga tcgcgaagag
     2881 atcccactac aaccggcggg ctggcagcca gaagaagatg aaaatcagtt ggatgagtta
     2941 cgactgaatg tggttgaagt gaaataacca ggatagcgcc cgatgcgcaa gcgtatcggg
     3001 ctactcttat ggaggccgga taagacgcgg ccagcgtcgc atccggcaat cccgaataag
     3061 atgtttactc ttgcacccgg caattcaaca tttcattatt ttaataaccg cacccggcac
     3121 gtttttcctt taatcttccc gccctgtaac tgtttccatg ctttatgagc aacagcctga
     3181 cggaccgcga catagacatg cgccggatgc acggcgattt tgccaatatc tgcgccatca
     3241 agcccgatat ctcctgtcag tgcacctaat acatcacccg ggcgcatttt ggcttttttc
     3301 ccgccatcga tacacaacgt tgccatttct gcttccagcg gcacaatgga actattagct
     3361 ggcggcgttt gccagttaag ttttatctgc aacatgtcag aaatgatatt ggcccgctgt
     3421 gcttcttccg gagcacagaa actgatcgcc agaccgctat ttcctgcacg agctgtacga
     3481 ccgatgcgat gtacatgaac ttcagggtcc cacgccagct caaagttcac caccagctca
     3541 agcgatttaa tatccagacc acgcgcagca acatcagtag caaccagtac acgggcgcta
     3601 ccgttagcaa aacgtaccag agtctgatcg cgatcgcgtt gctccagatc gccgtgtaac
     3661 gacaatgcac tttgccctac ttcattcagc gcgtcgcaga cagcctggca atcttttttg
     3721 gtattgcaaa acaccacgca agaggatggc tgatgcaagc ttaataaccg ttgcaacaga
     3781 ggaattttgc ctttgctgga tgtctcataa aattgttgtt caatgggtgg caaagcatct
     3841 gttgagtcaa tttcaatcgc caaaggatcg cgttgtactc gtccgctgat tgcggcgatg
     3901 gcttccggcc aggttgccga aaacagaagc gtctgtcgag atgcaggcgc aaaacggatg
     3961 acatcatcaa tggcatcgct aaatcccata tccagcatgc ggtcggcctc atccatcacc
     4021 aaagtattca gcgcatccag tgataccgtg cctttttgca agtgatccag caaacgaccc
     4081 ggcgttgcca cgataatatg cggcgcatgt tgcaacgaat cacgctgcat accgaacggt
     4141 tgaccaccgc acaacgtcaa aattttggta tttggcagaa aacgcgccag ccgacgcaat
     4201 tcacctgcca cctgatccgc cagttcacgc gtaggacaca gcactaaagc ctgggtttga
     4261 aatagcgacg catcaatttg ctgtaacaag ccgaggccaa aagccgccgt tttgccgctg
     4321 ccggttttcg cctgcacgcg aacatctttt ccggcaagga tcgccggaag cgcggcggcc
     4381 tgcaccggcg tcatggttaa ataacccaac tcattaaggt tcgtgagttg ggcgggaggc
     4441 aaaacattca gggtagaaaa agcggtcaca atctattctc gtggtcatcg acgcaaagtt
     4501 agcaggcgcg tatcctcgca gatctacgct cacgatgcga caatttaatc ggttcttcat
     4561 caggtggtgg gtcaggcatg ggttgcgggc gagggatcgg atcgggcact ggaacaggat
     4621 cgccaggaat cggttcaggg acaggaattt gcaaataaat aagtgtcgtc atatttccct
     4681 ccggtcattg ggtggactct taaagggtag acgctgataa ataacaggca aaaaaaagcc
     4741 gactcatcaa agtcggcgtc gtacgaatca attgtgctat gcagtaattc aaaaaaggaa
     4801 gtaagacaat atggagcgca acgcccatcg cttgacgttg cattcacctg caagggagat
     4861 attgccctga atgggtagag agtttattga cttcgctcaa actttgcggc gtttttgtat
     4921 acagacagcc ggaaaaattg cttttgttac aaccatttac tacgatgcaa ccataaagca
     4981 acaccaccaa taagaacaac taacagaata caaaaaattg aaaatccgaa ttgccacccg
     5041 ccgccaggga tcccaccaag gttgacgcca aataaccctg tcagaaaggt actgggtaaa
     5101 aagaccattg ccatcaatga cattgtatag gtacgacgag ctaaattttc ctgcatcacc
     5161 tgagcgattt catccgccat cacgccagtc cgtgctatac aggcgtcgat ttcgtcaaga
     5221 ccgcgcccaa ggcgatcggc aatatcctgc atccgacggc gttggtcatc gctcatccac
     5281 ggcaaacgtt cactggcaag acgagcataa acatcacgtt gcggtgccat atagcgacgc
     5341 atcacgatta attgtttgcg cagcagagcc aggaatccac gcggtggaat ttgctgatca
     5401 aggagattat cttcaaggtc gataatttta tcgtgcagct gctcgataaa ttcgctggaa
     5461 tgatcggtca acgcatcgca cacatccacc agccaccctc cgcaatcggt cggacccgtg
     5521 ccctcttcca gatcgctcac cacatcgtcc agcgccagca ctttgcgttg tcgggtcgaa
     5581 acaattaacc gcccgtccat atatacacgc atggcgacca gttgatcggg gcgttcatcg
     5641 gtgctgccgt ttatacagcg caatgtaatc agcgtgcctt caccgagacg gctgactcgg
     5701 ggccgagtgc tctcgcctgc cagcgcatca cgtacgttat tgggaagcag cggtgttgtc
     5761 gccagccatt gggcgctttc atggtgtaca taattaaggt ggagccagca gggatgcgct
     5821 tcatcaatca catctgtatt ttccagcggt ttaacgccgc ctctaccatc cagcatccag
     5881 gcaaatactg catccgggac attaacgtcc gatcccttaa tcgcttccac agtgcctcca
     5941 tcatcaacgc attattttgt agtctagcct tctggccctg ttacgcaaca tctcatcacc
     6001 ccattaccct gaaatgatta ataaaattct gtctaaattg aatac
//
LOCUS       DAZFXT010000206         5906 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.206, whole genome shotgun sequence.
ACCESSION   DAZFXT010000206 DAZFXT010000000
VERSION     DAZFXT010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5906
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.206"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(192..692)
                     /locus_tag="ACHKW0_004691"
     CDS             complement(192..692)
                     /locus_tag="ACHKW0_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HFO7477367.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDEADPELMFQDYEGFPGNMASECHINWAWVEGFRRARDEGCEEAYRLWVDDTGETD
                     FDSFRDAWWGEADSEEAFAVEFVSDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(1154..1750)
                     /locus_tag="ACHKW0_004692"
     CDS             complement(1154..1750)
                     /locus_tag="ACHKW0_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477368.1"
                     /translation="MMKSDEQYQVPVWMRPLLPLLCNTGGNDPEELLNDTETTASANI
                     VRYVLIVAVRSQIDLLQLLYRKGLLRTEIPGGFSPEEAQELLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMGVGNESPSAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(1747..2466)
                     /locus_tag="ACHKW0_004693"
     CDS             complement(1747..2466)
                     /locus_tag="ACHKW0_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001723065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HFO7477369.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYASAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENAI"
     gene            complement(2463..2897)
                     /gene="psiB"
                     /locus_tag="ACHKW0_004694"
     CDS             complement(2463..2897)
                     /gene="psiB"
                     /locus_tag="ACHKW0_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HFO7477370.1"
                     /translation="MKTELTLNALQSMNAQEYEDIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(2952..4910)
                     /locus_tag="ACHKW0_004695"
     CDS             complement(2952..4910)
                     /locus_tag="ACHKW0_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HFO7477371.1"
                     /translation="MPVTKCEPETTRKASRKSAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSVESVSELAESIKGVGLLQNLVVHTLPGDRYGVAAGGRRLAALNMLA
                     GRGILTADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNTKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVAEFLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAED
                     ERLNELMARYDSLENQCEESDLLAAEMKLIDCMAKVRAWTPEMRAGSGVVVSWRYGNV
                     CIQRGVQLRSEDDVADDADRTEQGLESASVEEISLPLLTKLTSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSKAHSSPARIRLECEHYALTSDAPSGKEGAAFMALMAEHSRF
                     AALLPEGWERDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGHTSRSPLDSLETAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNEAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCAPRPQTDATEHTANLADAA"
     gene            complement(4969..5202)
                     /locus_tag="ACHKW0_004696"
     CDS             complement(4969..5202)
                     /locus_tag="ACHKW0_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HFO7477372.1"
                     /translation="MSEYFRILQGLPDGPFTRKHAEAVAAQYRNVFIEDDHGEHFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHAIRDFGILK"
     gene            complement(5260..5787)
                     /locus_tag="ACHKW0_004697"
     CDS             complement(5260..5787)
                     /locus_tag="ACHKW0_004697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HFO7477373.1"
                     /translation="MSARGINKVILVGRLGNDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
BASE COUNT         1269 a         1847 c         1553 g         1237 t
ORIGIN      
        1 ggagccgaag ggtgtccccg cccccgggag tcagaacaac agaaacgaca aaacagaaaa
       61 atgcctcaca ctatgacagg caggaagggc agcgccgcag gcgcggccct cctgtatttc
      121 aggcaggcgt aagcctgcca gggttttccg ggggcctggc aggccatccc cccgccggag
      181 cggggagtaa atcaccgacg gaacacatga ccgtcaataa aggtgaagga gtccaggaat
      241 aaatcccgcg catacgcctc atagtcaaaa tagagcgcca ccgtctccgg cacgtcagcc
      301 agcaggccgg tatcactgac gaactcaacc gcaaaagcct cctcactgtc agcctcgccc
      361 caccaggcat cgcggaagct gtcaaaatcc gtctcaccgg tatcatccac ccagagacga
      421 taagcctctt cgcagccttc atcccgtgcc cggcggaagc cttcaaccca ggcccagttg
      481 atatgacatt cagaggccat attccccggg aaaccctcat aatcctgaaa catcagttca
      541 ggatcggctt catcctggtg aagagcacgg caggcggcga aaaagtcgcg ttcatcatca
      601 aacgtggtca ggtcaaacca gcggccagcg atgcttccac agttgtattt gtgccaggtt
      661 ccaacgtata cagcaggtgc aacaacagac attcccgaat cctccatagt cacatgttct
      721 gcgacaccgg gcggtgccgt gcctccatct ttgccgggcc tgcagggatg caagggcgca
      781 agggaagccg cagcccgacg cgcacggctg acgggagttg gagggtgaga ccgcgaaatc
      841 tgagcgggtg aacgaccgga aaatcacggc agacgggaag gagggaccgg gtgaccgcag
      901 cggttcatct taccttgcat cccgcccgca gaagcccggc tacgatggag gcacggcaca
      961 gcccgcacac gacacggcgg ccacataact gaaaacccac aaggccatgc cccgcatggc
     1021 cgggtaaaag cggagccgcc ggcgacttcc ttattccggc gagagcagag gacacgccgc
     1081 atgacctcca gaccacccct gatatgaaac gatcatgatc ggcgtgtccc tgcgtaaaat
     1141 atacatgcct ggttcaccgg agaatatctg tgatatccac aagttttctg tcgcgcagcc
     1201 agtcctgcgc ttcacgcacc acacaactgt ccggtttcac cgctttcaga gaagatgcca
     1261 gtcgcttgat caacatcacc agttcccgtg tctgtatgtc cggcattgtc tccggcggag
     1321 cagagggact ctcatttccg acgcccatcc catgcgcttc cagtgtggca cggacaaacc
     1381 aggcagcatc gaccagttgc tggcgaatcc aggccagatc ggcatttctg tcacgggctg
     1441 ccattcgctc acccgacagc gccttgctga tatggctgcg caccagatta tccaggagtt
     1501 cctgcgcttc ttccggtgaa aagccacctg gtatctctgt gcgcaacagt cctttcctgt
     1561 acagtagctg cagcagatca atctgcgacc gcactgcaac tatcagtaca taacggacaa
     1621 tattcgcact ggcggtggtt tctgtatcat tcagcagttc ctccggatca ttaccaccgg
     1681 tattgcagag caacggcaac agaggtcgca tccacacggg aacctgatat tgttcgtctg
     1741 atttcatcat attgcgttct ccagcttgtt cgggatcatc cactgctcaa gtgcacgtac
     1801 ctgtaccggt gcaccacgac cggcctcacc ggcttcaaaa atcagccgcc agagtggttc
     1861 ctcctgccag aaaaaccggt caaatgatga cagtgatgga aaacgcccgc accaccccca
     1921 gaaaatcgtt tccagatcat gctcctcaac aacaccagac cagcgggaca accagcttcc
     1981 gaccgtggcg ggagtatgaa acgcaaggtc aatctgcgcc tgagcaaaac atgtctggta
     2041 ttccagttcc cgtcgttctg cctcacgttc ccgctggcgg ctgtatttct gctctgtcag
     2101 cgtggttttc tgacgacgac gctcaccttc acgaaaagga acggacggga acagacgatg
     2161 ccccgcatcg gatggcaggg gaagaacaca gacttcacca aaactttcaa tcagtttatc
     2221 cacggcagcc agccacagga gacggttgcc atgaaattcc tccttcgtca gcgcaggggc
     2281 aaaaaagcgg agatcggtca gcgaaatccg gcgactgcca ttcaggcagc gaaagaaagc
     2341 gctggcataa ggccatgctg aaagtgtgcg tccctgacga cgacgttgtt cggtgacagc
     2401 cacagcctgc atggcggcct gttgttctgc gctgagggga atcagtgccc gtgaacgtgc
     2461 gctcatgcct gacctccttc tgccatcagg atatggatga tgtcgttaac actgtatccc
     2521 tgcgcatcaa gtgtcgcggc aagcgccagc gtgtgactga tagcgacagg cgtgaagata
     2581 tgttgcacct gaacgacggc gaaaggctgt ccaccaccat tcaccagaac cagcatccag
     2641 agctgagaga cgtctcccgg cgaacataat gccaggtgga aacgttcgtg ggctggcgtg
     2701 aaacgaacct ggacggggaa aaagcccccg aactcactgc catactcgcc attcatcatc
     2761 cagttagccg gtgcgtccac ttcacgcatc acctcgtgag tgagattacg gcgcatatcg
     2821 cttcccgcag cacggatatc ttcatattcc tgtgcgttca tggactgcag ggcattcagg
     2881 gtcagttcag ttttcatcac ggtatctccc ttgctgccgc cccgtctccg gcggggcggt
     2941 gtggttgttc atcaggcggc atcagccagg ttagcggtgt gttcagtggc atccgtctgt
     3001 ggacgtggtg cacacatcca gccaggcacc cagcggttgt ctttcatatg gaactctgca
     3061 tgttcagccg catcgccttt cttcattttc tccgcgtccc gtgcggcacc ggacagtccg
     3121 gcctcattca gggctgcgat aatctgcggc tttttcaggt gtccgaagaa gtttgctttt
     3181 gtcggctgcc accagtcgcg catgtgaaag ccgatggcgg tttccagcga gtccagcgga
     3241 ctgcgtgacg tgtgaccaca ctcgcgggtc tggacaccgt taagactgca cgcggtacag
     3301 aaactgagca gggataacag cacctcctgg ctaagtgaca ggaacgtcgt catatcccgt
     3361 tcccatcctt ccggcagcag ggcagcaaaa cgagagtgtt ctgccatcag cgccatgaat
     3421 gcggcacctt ccttccctga cggtgcatcg ctggtcagcg cataatgctc acattccagg
     3481 cgaattctgg cgggactact atgcgctttg ctgtcaaaca cattcaggca gagcgtccat
     3541 gccagcagtg ccagggattt gtccggttgc tgcatcagtg ctgcctggac tgccagcgtg
     3601 cgctctgagg tcaatttagt cagcaacggc agactaattt cctccactga cgcgctctcc
     3661 agcccctgtt ccgtgcggtc agcgtcgtca gccacatcat cctcactgcg caactgcaca
     3721 ccacgctgga tacacacatt accgtaacgc caggacacca ccacgccact tccggcacgc
     3781 atctccggcg tccacgccct gaccttcgcc atgcagtcaa tcagcttcat ttctgctgcc
     3841 agcaggtcag attcctcaca ctggttttcc agcgagtcgt aacgagccat cagttcgttc
     3901 agacgttcgt cttccgcctc cgtcagcacc gcttccggct ccggcagaca gcggtatgtt
     3961 ccggcatcct cacggcactc accgacaggc tccatgcgcc cggcgcacca ttcccagcct
     4021 tcggcttccc gaaggaactc tgcaacagcc tggagttttt ccagcagggc ggcatcgagc
     4081 gccacgcagt ccacatagcc accctcgtcg tcgctgaaca agtcagtgcg cagttcatct
     4141 ggcgagaagg catcagcccc cacaaagcgg aatttagtgt tccccgccac cgccacttca
     4201 ctttcggtca ccagacgacg aatggtctgt acttccggtt taccgcccca tcccgactga
     4261 caggcggctt caaacacctg cacctgacgc gcggtgtcgt tctccagcgc cagcgcctga
     4321 cagtgctctg tggtgatgcg gtcttctgcc agcgcatcga ggatgacagg cgcaaggtct
     4381 gccagtttca gcattcgctg aacgtggcgg ggtgaatagc ccagcaaatc accgatttgt
     4441 gcaggcgttt tgccttcctg cgccattgcg cggaatccgg caatctgttc ggcagggtgc
     4501 atatcccgac gatgaccgtt ttcagtcatt gatgcggcag tcgccagttc ctgcggaata
     4561 accttcacgc gtacaggcca gtcagccgta aggatgccac gccctgccag catgttgagt
     4621 gcagccagtc ggcgaccacc tgcggcgaca ccgtaacggt caccaggcag ggtatgcacg
     4681 accagattct gcaacaggcc aacgccctta atggactcag ccagttcgct gacggactcc
     4741 acagaatacg gcaccgtgcg cacattcagc ggtgatttaa tcagtgaggc cagcggcacg
     4801 ctcacttcct ccgtctgcgc cagcagggca gacaggacag tttcctgcgt ttttgcagat
     4861 ttacggcttg ctttgcgggt ggtttctggt tcacacttcg ttacaggcat aatcatcccc
     4921 ttataattgc ttatgtcctg ccggacgttc gcgcgtccgg cacctctctt acttaagaat
     4981 cccgaaatca cggatggcat ggttcatcca gtacccggca ccgtcctcaa aattccaggt
     5041 acgccagacc attgttccgt tattacggac aaccaggcga aaatgttctc cgtggtcatc
     5101 ctcgataaag acgttccggt actgtgcggc aacggcttcg gcatgtttgc gggtaaaagg
     5161 accgtcaggc agtccctgaa gtattctgaa atattcgctc atcctcatat ctccggtgat
     5221 gccccgcgca gggcggggcg gttatcacag tcaggccagt cagaacggga tatcgtctga
     5281 aaacccgtaa tcctccccct ccggcggttg cggctgaggc tcgggctgcg cggccttacg
     5341 tccacggcct ttcgttttcg cgccaccttt tttcgtcgcg tcagcactct gtggctgccc
     5401 attctgctga ggcttcggtt gcgcctgagc gttctgctgt ggtgcacgtc ccagcatctg
     5461 cacggtgccc gtggtcttaa caagaatttc agtgacgtaa cgggtgatac cgttatcttc
     5521 ccagctacgg gtgcgaagct gaccttcgat gtagacctgc gcacccttgc gcagatattc
     5581 acctgccact tccgcgagct tgccgaacag caccacgcgg tgccattccg tctgctcccg
     5641 catctccccc gtctgtttgt cacgccagct ttctgatgtg gccacctgca ggtttgccac
     5701 tgcgcccccg ttggggatgt aacggacttc cggatcattg cccagacgcc cgacgaggat
     5761 gaccttgttg ataccacgtg cactcataag aacctcctga ctgaaagatg tgagataaac
     5821 cggataccgg cgacgggacg ttcatgtgcc ccttcagagg agcgaaagct cctcctgccg
     5881 tccacttcac aggggtttct ccccgc
//
LOCUS       DAZFXT010000207         5886 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.207, whole genome shotgun sequence.
ACCESSION   DAZFXT010000207 DAZFXT010000000
VERSION     DAZFXT010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5886
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.207"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..461
                     /locus_tag="ACHKW0_004698"
     CDS             <1..461
                     /locus_tag="ACHKW0_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7477374.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRA
                     QAREQFTGAVDRIFDTAESVCVASVARTVLRAVRDTVVSVARAVWNWIFF"
     gene            832..3681
                     /gene="cah"
                     /locus_tag="ACHKW0_004699"
     CDS             832..3681
                     /gene="cah"
                     /locus_tag="ACHKW0_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding autotransporter Cah"
                     /protein_id="HFO7477375.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VTSVPALAADKVVQAGETVNDGTLTNHDNQIVFGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGLARDTLIYAGGDQSVHGRALNTTLNGGYQYVHRDGLALNTVINEGGW
                     QVVKAGGAAGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVIGTNRLGNFTV
                     ENGKADGVVLESGGRLDVLESHSAQNTLVDDGGTLAVSAGGKATSVTITSGGALIADS
                     GATVEGTNASGKFSIDGTSGQASGLLLENGGSFTVNAGGQAGNTTVGHRGTLTLAAGG
                     SLSGRTQLSKGASMVLNGDVVSTGDIVNAGEIRFDNQTTPNAALSRAVAKSNSPVTFH
                     KLTTTNLTGQGGTINMRVRLDGSNASDQLVINGGQATGKTWLAFTNVGNSNLGVATTG
                     QGIRVVDAQNGATTEEGAFALSRPLQAGAFNYTLNRDSDEDWYLRSENAYRAEVPLYT
                     SMLTQAMDYDRILAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGATPESSGS
                     YGFVRLEGDLLRTEVAGMSLTTGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLN
                     LVHTSSGLWADIVAQGTRHSMKASSDNNDFRARGWGWLGSLETGLPFSITDNLMLEPQ
                     LQYTWQGLSLDDGQDNAGYVKFGHGSAQHVRAGFRLGSHNDMTFGEGTSSRDTLRDSA
                     KHSVSELPVNWWVQPSVIRTFSSRGDMSMGTAAAGSNMTFSPSRNGTSLDLQAGLEAR
                     IRENITLGVQAGYAHSVSGSSAEGYNGQATLNMTF"
     gene            3792..>5886
                     /locus_tag="ACHKW0_004700"
     CDS             3792..>5886
                     /locus_tag="ACHKW0_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HFO7477376.1"
                     /translation="MHEKNIALLCDEADRLLQLNINLLRQMVDEPDVLSDSKNENGLL
                     FDKRKALKRIEELEGEQIKTARREMVLAVVGTMKAGKSTTINAIVGQEILPNRNRPMT
                     SVPTLIRHVPGKTEPVLHLEHIQPVRNLLITLQEKLATPAGQQVAQSLQQTGDTRELL
                     DILADDVWLKNEYHGEDEIFTGLASLNDLVRLAAAMGTEFPFDEYAEVQKLPVIDVAF
                     SHLVGMDACQGTLTLLDTPGPNEAGQPQMEMMMRDQLQKASAVLAVMDYTQMNSKADE
                     EVRKELNAIADVSAGRLFVLVNKFDEKDRNGDGADAVRQKVPAMLNSDVLPASRVYPG
                     SSRQAYLANRALHELRKNGALPVDEAWVDDFIREAFGPMVEEDDWKDSTKVNKKAEKL
                     WNISLIDQLITEVIQSSHSRAAALAVDSAAAKLMQNAENVSEYLSLRHQGLQQSIQSL
                     QAHITSLLADIQEIEECQNQVTGDVRMAMEDINTKTGELLTKVCASLEEELNDYFRSG
                     KRKEQQMLEEENSAQPRERNAFAFFHDIFGTGNQHDRMRDFDPDSPEIKFSDRRAALE
                     LMTQIESTVTSLHREAEAQFRPELEKIVSGIETGFRGTALYATENIAGRINARLEDEG
                     FTVKISFPAVSQLQTRLAVKINLSALMEERTETVTRRRRQSGVWGTVCRWFGTSDLGW
                     ENYDEDVSRSVININK"
BASE COUNT         1470 a         1503 c         1712 g         1201 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggca ttcagccttc tcccgcacag gcacagaaca
      121 ttcgggagaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgttgccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctaatg acagcacttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccgcgctca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tgttgcctct gttgcccgta cggttctgcg tgccgtccgt gacacggtgg
      421 tctctgttgc ccgcgctgta tggaactgga ttttcttctg aacagtctgc cagggaaatg
      481 cctgataaca ttacgttaac caactgaaaa catgacacaa acccctcata ctcctcctgt
      541 ttctcttgcg tgaccgcctt gtcgttaata gaataaaacg atcgataaaa caggtatcga
      601 tcgttgctat cgatcgttta tagtggttaa tggtctgcga aaaggcagct gaatctcttc
      661 atcatgcaga acggaattgc acacaacaga ctgatacttc tctgtctgat gaagggggag
      721 agcgtttacg cccgggacgt atcgctgtgc ccgataactc tgttttgtac ctgccggtat
      781 ccacttttgt gggtaccggc ttttttattc accctctgta aggaaaagct gatgaaacga
      841 catctgaaca ccagctacag gctggtatgg aatcacatta cgggcaccct ggtggtggcc
      901 tccgaactgg cccgctcacg gggaaaacgc gccggtgtgg cggttgcgct gtctcttgct
      961 gctgtcacat cagtcccggc actggctgct gacaaggttg tacaggcggg agaaaccgtg
     1021 aacgatggaa cactgacaaa tcatgacaac cagattgtct tcggtacggc caacggaatg
     1081 accatcagta ccgggctgga actggggccg gacagtgaag aaaacaccgg tgggcaatgg
     1141 atacagaatg gcgggatagc cggaaacacc actgtcacca caaatggtcg tcaggtcgtg
     1201 ctggaggggg gaacagccag tgatacggtt attcgtgacg gcgggggaca gagcctgaac
     1261 ggactggcgg tgaacaccac actgaataac agaggcgagc agtgggtgca tgagggcggg
     1321 gttgccaccg gtacaattat caaccgcgac ggttaccaga gcgttaaaag tggcgggctg
     1381 gcaacaggaa ccatcatcaa caccggcgca gaaggcggcc ctgattctga caactcgtat
     1441 acgggtcaga aggtccaggg aacagcagaa tccaccacca tcaacaaaaa tggacggcag
     1501 attatcttat tttccgggct agcccgtgac actctcattt acgcaggtgg tgaccagtcg
     1561 gtacacggaa gggccctgaa taccacactg aatggcggtt accagtatgt gcacagggac
     1621 ggacttgcgc tgaacacggt aattaacgag gggggctggc aggttgttaa ggcaggtggc
     1681 gctgccggta acaccaccat aaatcagaac ggtgaactga gggtacatgc cggcggggaa
     1741 gccactgcag tcacccagaa cacgggcggt gcactggtta ccagtactgc tgcaactgtc
     1801 atcggcacaa accgtctggg gaatttcacg gtggaaaacg gtaaggctga cggtgttgtt
     1861 ctggaatccg gcggtcgtct ggatgtactg gagagccatt cagcacagaa taccctagtg
     1921 gatgacggcg gtaccctggc agtgtctgcc ggcggtaagg cgacaagtgt caccataaca
     1981 tccggtggtg ccctgattgc agacagtggt gccactgttg aggggaccaa tgccagcggt
     2041 aagttcagta ttgatggcac atccggtcag gccagcggcc tgctgctgga aaatggcggc
     2101 agctttacgg ttaatgccgg gggacaggct ggcaacacca ctgtcggaca tcgtggaaca
     2161 ctgacgctgg ctgccggggg aagtctgagt ggcagaacac agctcagtaa aggcgccagt
     2221 atggtactga atggtgatgt ggtcagtacc ggcgatattg ttaacgcagg ggagattcgc
     2281 tttgataatc agacgacacc gaatgccgcg ctgagccgtg ctgttgcaaa aagtaactcc
     2341 ccggtaacgt tccataaact gaccaccacg aacctcaccg gccagggcgg caccatcaat
     2401 atgcgtgttc gccttgatgg cagcaatgcc tctgaccagc tggtgattaa tggtggtcag
     2461 gcaaccggca aaacctggct tgcgtttaca aatgtcggaa acagcaacct cggggtggca
     2521 accaccggac agggtatccg ggttgtggat gcacagaatg gcgccaccac agaagaaggt
     2581 gcgtttgccc tgagtcgccc gcttcaggcc ggcgccttta actacaccct gaaccgtgac
     2641 agcgatgaag actggtacct gcgcagtgaa aatgcttatc gtgctgaagt ccccctgtat
     2701 acatccatgt tgacacaggc aatggactat gaccggattc tggcaggctc ccgcagccat
     2761 cagaccggtg taaacggtga aaataacagc gtccgtctca gcattcaggg cggtcatctc
     2821 ggtcacgata acaacggcgg tattgcccgt ggagccacgc cggaaagcag cggcagctat
     2881 ggcttcgtcc gtctggaggg tgacctgctc agaacagagg ttgccggtat gtctctgacg
     2941 acaggggtgt atggtgctgc aggccattct tccgttgatg ttaaggatga tgacggttcc
     3001 cgcgccggca cggtccggga tgatgccggc agtctgggcg gatacctgaa tctggtacac
     3061 acatcctccg gcctgtgggc tgacattgtg gcccagggaa cccgtcacag catgaaagcg
     3121 tcatcggaca ataacgactt ccgcgcccgg ggctggggct ggctgggctc actggaaacc
     3181 ggtctgccct tcagtatcac tgacaatctg atgctggagc cacaactgca gtacacctgg
     3241 cagggactct ccctggatga cggccaggat aacgccggtt atgtgaagtt cgggcatggc
     3301 agtgcacaac atgtgcgtgc cggtttccgt ctgggcagcc acaacgatat gacctttggt
     3361 gaaggcacct catcccgtga caccctgcgc gacagtgcaa aacacagtgt gagtgaactg
     3421 ccggtgaact ggtgggtaca gccttctgtt atccgcacct tcagctcccg gggtgacatg
     3481 agcatgggga cagccgcagc cggcagtaac atgacgttct caccgtcccg gaatggcacg
     3541 tcactggacc tgcaggccgg actggaagcc cgtatccggg aaaatatcac cctgggcgtt
     3601 caggccggtt atgcccacag cgtcagcggc agcagcgctg aaggctataa cggtcaggct
     3661 acgctgaata tgactttctg attatacggt atcccggaaa tacgatttca ggaaaattca
     3721 tgcagtaatg ggaaaggacg ctcattgctg ttcaccgcag ttctgaatta ttgaatttat
     3781 aaaggtgaat gatgcacgaa aagaacatcg ccctgctttg tgatgaagcc gaccggcttt
     3841 tgcaactgaa tatcaatctg ctccggcaaa tggttgatga gccggatgtg ttatctgaca
     3901 gtaagaacga aaacgggctg ctttttgata aacggaaagc gctgaaaaga attgaggagc
     3961 tggagggcga acaaatcaaa accgcccgca gggagatggt gctggctgtg gtcggcacga
     4021 tgaaggcggg caaatcaacc accatcaacg ccattgtggg gcaggaaatt ctgcccaacc
     4081 gtaaccgccc catgacctcg gtaccgacat tgatccgcca cgttcccgga aaaacggaac
     4141 cggtcctcca tctggaacat attcagcccg tccgcaattt attaatcaca ctgcaggaaa
     4201 aactcgccac cccggcaggg cagcaagtcg cacagtccct gcagcaaacc ggggataccc
     4261 gcgaactact ggatattctg gcggatgatg tctggcttaa aaatgaatac cacggggaag
     4321 acgaaatctt taccggactg gcatcgttaa atgacctggt tcgtcttgct gcggcaatgg
     4381 ggactgaatt tccttttgac gaatacgcag aagtgcagaa actaccggtg atcgacgtgg
     4441 cgttcagcca cctggtggga atggatgcat gtcagggaac actcacactg ctggataccc
     4501 ccggccctaa tgaagccgga caaccacaga tggaaatgat gatgcgggat caactacaga
     4561 aagcctctgc ggtgctggcg gtgatggatt acacccagat gaactcgaaa gccgatgagg
     4621 aggttcgtaa agagcttaat gccattgctg acgtctcagc cggtcgtctg tttgtgctgg
     4681 tcaataaatt cgatgaaaaa gatcgcaatg gtgatggtgc ggatgccgta cgccagaagg
     4741 tgccagcaat gctgaacagc gatgtgctgc ccgcctcccg cgtttatccc ggctcatcac
     4801 gtcaggcgta cctggctaac cgtgcgcttc atgagttacg aaaaaacggt gcgcttccgg
     4861 ttgatgaagc ctgggtcgat gattttatca gggaagcttt cggacctatg gtggaagagg
     4921 acgactggaa agacagcact aaggtaaata aaaaggcaga aaaactctgg aatatatctc
     4981 tgatcgatca actgataacg gaagtcatac agagctccca ttccagagcg gcggcactgg
     5041 cggttgactc tgccgccgca aaactgatgc agaatgcaga aaatgtcagc gaatatctgt
     5101 cattacgtca tcaggggctg cagcaaagca ttcagtcact acaggcgcat attaccagcc
     5161 tgctggcgga tatccaggag attgaggaat gtcagaatca ggtgaccggt gatgtcagaa
     5221 tggccatgga ggacatcaat accaaaaccg gggaattact gacgaaggtc tgtgcctcac
     5281 tggaggagga gctgaacgac tatttcagaa gcggtaaacg caaagaacag caaatgctgg
     5341 aggaagaaaa ttcagcgcag ccccgggaga gaaacgcatt cgctttcttt cacgatattt
     5401 tcggaaccgg aaatcagcat gacaggatgc gggactttga tccggacagc cccgaaataa
     5461 aattcagtga tcgcagggca gcccttgaac tgatgacgca aatcgaatcg accgtgacca
     5521 gcctgcaccg tgaggctgaa gcacagttcc ggcctgagct ggagaaaatc gtcagcggga
     5581 ttgaaacagg ttttcgtggc acggccctgt acgccacaga aaacattgcc ggtcgcatca
     5641 atgcccgcct ggaggatgag ggctttaccg taaaaatcag ttttccggca gtcagccagt
     5701 tacagacccg gctcgcggta aaaataaatc tgagtgcgct tatggaggaa agaacggaga
     5761 ccgtcacccg tcgccgtcgg cagagtggcg tatggggaac cgtttgtcga tggtttggca
     5821 ccagtgacct gggctgggaa aactatgacg aggatgtgag tcgcagcgtg atcaatatca
     5881 acaagg
//
LOCUS       DAZFXT010000208         5864 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.208, whole genome shotgun sequence.
ACCESSION   DAZFXT010000208 DAZFXT010000000
VERSION     DAZFXT010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.208"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            88..1239
                     /gene="yiaY"
                     /locus_tag="ACHKW0_004701"
     CDS             88..1239
                     /gene="yiaY"
                     /locus_tag="ACHKW0_004701"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HFO7477377.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1404..2942
                     /gene="aldB"
                     /locus_tag="ACHKW0_004702"
     CDS             1404..2942
                     /gene="aldB"
                     /locus_tag="ACHKW0_004702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HFO7477378.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3161..3316)
                     /locus_tag="ACHKW0_004703"
     CDS             complement(3161..3316)
                     /locus_tag="ACHKW0_004703"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477379.1"
                     /translation="MAKLMNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLIRP
                     LTIETRD"
     gene            3487..3810
                     /locus_tag="ACHKW0_004704"
     CDS             3487..3810
                     /locus_tag="ACHKW0_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HFO7477380.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQNHDSSVVQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3816..4952
                     /locus_tag="ACHKW0_004705"
     CDS             3816..4952
                     /locus_tag="ACHKW0_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HFO7477381.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4949..>5864)
                     /locus_tag="ACHKW0_004706"
     CDS             complement(4949..>5864)
                     /locus_tag="ACHKW0_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HFO7477382.1"
                     /translation="ENISHAATVLGIAQANVSKYLADFESKVGLKVFDRTTRQLMLTP
                     FGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYAPTGIITYLSKHVIDKIKDIG
                     DITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDESLVASFITQYAVTAYASQRY
                     LEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGSKEVRDYRVKGNYVCDNTQSA
                     LELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWWLDLVAIFRKREYQPWRVQYV
                     LDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1449 a         1402 c         1532 g         1481 t
ORIGIN      
        1 ttttgataat caataataat gatcatgttt tcgaatgaaa atcctcagta agctgcccac
       61 ccttttttac actttcagga gtgtgttatg gcatcttcaa ctttctttat tccttctgtg
      121 aatgtcatcg gcgctgattc attgactgat gcaatgaata tgatggcaga ttatggattt
      181 acccgtacct taattgtcac tgacagtatg ttaacgaaat taggtatggc gggcgatgtg
      241 caaaaagcac tggaagaacg caatattttt agcgttattt atgatggcac ccaacctaac
      301 cccaccacgg aaaacgtcgc cgcaggtttg aaattactta aagagaataa ttgcgatagc
      361 gtgatctcct taggcggtgg ttctccacac gactgcgcaa aaggtattgc gctggtggca
      421 gccaatggcg gcgatattcg cgattacgaa ggcgttgacc gctctgcaaa accgcagctg
      481 ccgatgatcg ccatcaatac cacggcgggt acggcctctg aaatgacccg tttctgcatc
      541 atcactgacg aagcgcgtca tatcaaaatg gcgattgttg ataaacatgt cactccgctg
      601 ctttctgtca atgactcctc tctgatgatt ggtatgccga agtcactgac cgccgcaacg
      661 ggtatggatg ccttaacgca cgctatcgaa gcgtatgttt ctattgccgc cacgccgatc
      721 actgacgctt gtgcactgaa agccgtgacc atgattgccg aaaacctgcc gttagccgtt
      781 gaagatggca gtaatgcgaa agcgcgtgaa gcaatggctt atgcccagtt cctcgccggt
      841 atggcgttca ataatgcttc tctgggttat gttcatgcga tggcgcacca gctgggcggt
      901 ttctacaacc tgccacacgg tgtatgtaac gccgttttgc tgccgcatgt tcaggtattc
      961 aacagcaaag tcgccgccgc acgtctgcgt gactgtgccg ctgcaatggg cgtgaacgtg
     1021 acaggtaaaa acgatgcgga aggtgctgaa gcctgcatta acgccatccg tgaactggcg
     1081 aagaaagtgg atatcccggc aggcctacgc gacctgaacg tgaaagaaga agatttcgcg
     1141 gttctggcga ctaatgccct gaaagatgcc tgtggtttta ctaacccgat ccaggcaact
     1201 cacgaagaaa ttgtggcgat ttatcgcgca gcgatgtaat catcatttcc acaacggctg
     1261 gcaaattgtc agccgctttt tcaactatct ctgtaaccct tgcccgtaaa ttcgtgatag
     1321 ctgtcgtaaa gctgttaccg actggcgaag atttcgccag tcacgtctac ccttgttata
     1381 cctcacaccg caaggagacg atcatgacca ataatccccc ttcagcacag attaagcccg
     1441 gcgagtatgg tttccccctc aagttaaaag cccgctatga caactttatt ggcggcgaat
     1501 gggtagcccc tgccgacggc gagtattacc agaatctgac gccggtgacc gggcagctgc
     1561 tgtgcgaagt ggcgtcttcg ggcaaacgag acatcgatct ggcgctggat gctgcgcaca
     1621 aagtgaaaga taaatgggcg cacacctcgg tgcaggatcg cgcggcgatt ctgtttaaga
     1681 ttgccgatcg aatggaacaa aacctcgagc tgttagcgac agctgaaacc tgggataacg
     1741 gcaaacccat tcgcgaaacc agtgctgcgg atgtaccgct ggcgattgac catttccgct
     1801 atttcgcctc gtgtattcgg gcgcaggaag gtgggatcag tgaagttgat agcgaaaccg
     1861 tggcctatca tttccatgaa ccgttaggcg tggtggggca gattatcccg tggaacttcc
     1921 cgctgctgat ggcgagctgg aaaatggctc ccgcgctggc ggcgggcaac tgtgtggtgc
     1981 tgaaacccgc acgtcttacc ccgctttctg tactgctgct aatggaaatc gtcggtgatt
     2041 tactgccgcc gggcgtggtg aacgtggtca acggcgcagg tggggaaatt ggcgaatatc
     2101 tggcgacctc gaaacgcatc gccaaagtgg cgtttaccgg ctcaacggaa gtgggccaac
     2161 aaattatgca atacgccacg caaaacatta ttccggtgac gctggagctg ggcggcaaat
     2221 cgccaaatat cttctttgct gatgtgatgg atgaagaaga tgcctttttc gataaagcgc
     2281 tggaaggctt tgcactgttt gcctttaacc agggtgaagt ttgcacctgt ccgagtcgtg
     2341 ccttagtgca ggagtcaatc tacgaacgct ttatggaacg cgccatccgc cgtgtcgaaa
     2401 gcattcgcag cggtaaccca ctcgacagcg tgacgcaaat gggcgcgcag gtttctcacg
     2461 ggcaactgga aaccatcctc aactacattg atatcggtaa aaaagagggc gcagacgtgc
     2521 tcaccggcgg gcggcgcaag ctgctggaag gtgaactgaa agacggctac tacctcgaac
     2581 cgacgattct gtttggtcag aacaatatgc gggtgttcca ggaggagatt tttggcccgg
     2641 tgctggcggt gaccaccttc aaaacgatgg aagaagcgct ggagctggcg aacgatacgc
     2701 aatatggcct gggcgcaggc gtctggagtc gtaacggtaa tctggcctat aagatggggc
     2761 gcggcattca ggccgggcgc gtgtggacca actgctatca cgcttacccg gcacatgctg
     2821 cgtttggtgg ctacaaacaa tcaggtatcg gtcgcgaaac ccacaagatg atgctggagc
     2881 attaccagca aaccaagtgc ctgctggtga gctactcgga taaaccgttg gggctgttct
     2941 gatataagaa gctggtcgca ttgggtattc attgcctgat gcgacgctta cgcgtcttat
     3001 catgcctacg ggaacctgac cgtaggccgg ataaggcgta cacgccgcat ccggcataaa
     3061 caaagcgcac gttgttacaa tttacgtttt aaacctacgt gcgcaataac ccagactacg
     3121 ccaacagaag tgcgtttctg gggcgtgttt tgtcaggagt ttaatctcgc gtttcgatgg
     3181 tgagcgggcg aatcaggccg taaatcacca ttggtgatcc gaggccaatc agcaataaac
     3241 ggtagataat tgaaggttta atacgcttaa tctgtactgt ggatgtttta ctggagttat
     3301 tcattagttt ggccaatagt tccattattt caatatgata agtctcgacc tgttgaaaag
     3361 ccaggtagca cacttcagaa agtatataaa aaacaatatg tcgttatata aataaggaaa
     3421 ttgcactgca tcatggcatt tcataatatg cgttaattgt tcttttttat attctctgag
     3481 ggaaatatgt tcctggacta ttttgcactg ggagtgctta tttttgtatt tctggtgatc
     3541 ttctatggga ttattatttt acatgatatt ccctacctga ttgctaaaaa acgtaatcat
     3601 cctcatgccg acgccattca tgttgctggt tgggtgagtc tttttacgtt acatgttatc
     3661 tggccatttt tgtggatttg ggccacgctt taccgcccgg agcggggatg gggaatgcaa
     3721 aaccatgact cctccgttgt gcagctacaa cagcgtattg ccgggctgga gaaacagctc
     3781 gccgacatca aatcctcttc tgccgagtaa tacttatgga tttactgatt attttgacct
     3841 atgtggcttt cgcatgggca atgtttaaga tcttcaaaat tcctgtaaat aaatggacca
     3901 ttcccacagc ggccctgggt ggcatattta ttgtcagtgg tttaattctg ttaatgaact
     3961 ataaccatcc gtataccttc aaagcgcaaa aagcggttat ttctattcct gttgtcccac
     4021 aggtgacagg cgtggtgatc gaagtgacgg ataagaaaaa tacgctgatt aaaaaaggtg
     4081 aggtgctatt tcgactggac ccgacgcgtt atcaggcgcg ggtggatcgg ctgatggcgg
     4141 atatcgttac cgcagaacat aaacagcggg cgttgggcgc agagttagat gagatggcgg
     4201 cgaatactca gcaggcaaag gccacgcggg ataaattcgc taaagagtat cagcgttacg
     4261 cacgcggcag ccaggcgaaa gtaaacccgt tttcagaacg cgatattgat gtggcgcggc
     4321 aaaattatct ggcgcaggaa gcctccgtaa agtcatcggc ggcggaacaa aaacagatcc
     4381 agagccagct ggatagcctg gtgttgggtg aacattctca aatcgccagc ctgaaagcgc
     4441 agctcgcgga agcaaaatat aaccttgagc agacgatagt gcgtgcgccg agcgatggtt
     4501 atgtgaccca ggtgctgatt cgtccgggta cctatgccgc gtcgctgccg ctacgtccgg
     4561 tgatggtgtt tatacccgat cagaaacgac aaattgtggc gcagttccgt cagaactcct
     4621 tgctgcgact ggcccctggc gatgatgcgg aagtggtatt taacgctctg ccaggtaagg
     4681 tattcagcgg caagctggca gccattagtc cagctgttcc cggcggagct tatcagtcga
     4741 ccggcacctt acagacgtta aacacagcgc cgggttcaga tggcgttatc gcgaccattg
     4801 aactggatga gcacactgat ttgagcgctt taccagacgg tatttacgcc caggtggcgg
     4861 tctactctga tcatttcagc catgtctcgg tgatgcgcaa agtgctgtta cgcatgacca
     4921 gttgggtgca ttacctttat ctcgatcatt aatcttcgct ttccgccgct tgttcaggcc
     4981 gcagttgctg cgactgagca agctggtggc gaatttcacg cagcatttca tccagcacat
     5041 actggacccg ccagggctgg tattcccgtt tacggaagat agccaccaga tccagccacc
     5101 attcatacgg gtgctgaaag cagggcacca gcgtgccgtc ttgcaggtcg ctctgcacac
     5161 ttttgtctgg cgcaaacaca atccccaggt gatttcgtgc cagctccagc gccgattgcg
     5221 tgttatcgca aacgtaattt cccttcacgc gataatcccg cacctcttta ctcccggcga
     5281 cgttgaagcg ccagatattc gcgtcgtcaa tcatcatcga gtcgatcaaa atacaggagt
     5341 gatgttcgag ttcgtcagga cggctaatag gatgtttctc aagatagcgc tggctggcat
     5401 aagcggttac ggcatattgg gtaataaaac tggcaaccag cgattcatct tttggtggag
     5461 cgtagctgat taaaacatca caatcatcag gaaattcgac gccttcataa aaggcattac
     5521 gctcaagatt gcaggttttt agcgataagg tgatatcacc gatatcttta attttatcga
     5581 ttacatgttt ggataaatag gtaattatgc cggttggggc gtagatagtg acccgaccac
     5641 gtttctcatg cttataatct gcaataaaat tattaagttg ctcgtttcta tccagcatgt
     5701 cgttgatgta cggtaacagc gcggtgccaa aaggtgtgag catcagctgc cgggtggttc
     5761 ggtcaaagac ttttaaaccc acttttgatt caaaatcagc aagatatttg ctgacgttgg
     5821 cctgtgcgat gccaagtaca gtcgcggcat ggctgatatt ttca
//
LOCUS       DAZFXT010000209         5835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.209, whole genome shotgun sequence.
ACCESSION   DAZFXT010000209 DAZFXT010000000
VERSION     DAZFXT010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.209"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            66..806
                     /gene="yiaT"
                     /locus_tag="ACHKW0_004707"
     CDS             66..806
                     /gene="yiaT"
                     /locus_tag="ACHKW0_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0030674 - protein-macromolecule adaptor
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HFO7477383.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNENT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDHAMQQVDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
     gene            complement(753..1016)
                     /locus_tag="ACHKW0_004708"
                     /pseudo
     CDS             complement(753..1016)
                     /locus_tag="ACHKW0_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239594.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1020..1121
                     /locus_tag="ACHKW0_004709"
                     /pseudo
     CDS             1020..1121
                     /locus_tag="ACHKW0_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(1153..1848)
                     /gene="araD"
                     /locus_tag="ACHKW0_004710"
     CDS             complement(1153..1848)
                     /gene="araD"
                     /locus_tag="ACHKW0_004710"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008742 - L-ribulose-phosphate 4-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0019568 - arabinose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HFO7477384.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDETRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(1842..2702)
                     /gene="sgbU"
                     /locus_tag="ACHKW0_004711"
     CDS             complement(1842..2702)
                     /gene="sgbU"
                     /locus_tag="ACHKW0_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HFO7477385.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDDAVRQRAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEMMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTE
                     QSPGQFRDVPFGEGCVDFVGIFKTLHKLNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            complement(2695..3357)
                     /gene="ulaD"
                     /locus_tag="ACHKW0_004712"
     CDS             complement(2695..3357)
                     /gene="ulaD"
                     /locus_tag="ACHKW0_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HFO7477386.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHRGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            complement(3354..4850)
                     /gene="lyxK"
                     /locus_tag="ACHKW0_004713"
     CDS             complement(3354..4850)
                     /gene="lyxK"
                     /locus_tag="ACHKW0_004713"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HFO7477387.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGGVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            complement(4854..>5835)
                     /gene="yiaO"
                     /locus_tag="ACHKW0_004714"
     CDS             complement(4854..>5835)
                     /gene="yiaO"
                     /locus_tag="ACHKW0_004714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter
                     substrate-binding protein YiaO"
                     /protein_id="HFO7477388.1"
                     /translation="LRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAAKK
                     FNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMNLL
                     DVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTPAD
                     LKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAKFF
                     EVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQKI
                     IDGMKEAGVEVITDLDRKAFSDALGNQVRDMFVKDVPQGADLLKAVDEVQ"
BASE COUNT         1249 a         1600 c         1602 g         1384 t
ORIGIN      
        1 cggagaaatt gtaagcatta ctatgccgac tcttttttat cttattttaa aatggaatac
       61 cggacatgtt aattaaccgc aatattgtgg cgttatttgc gttgcctttt atggcaagcg
      121 caactgcttc tgaattatcc attggtgccg gtgcggctta taatgaatcg ccttatcgcg
      181 gttataatga aaatacaaag gcaattcccc tgattagtta tgaaggtgat tctttttatg
      241 ttcgtcagac cacgttaggt tttattctgt cgcaaagtga aaaaaatgaa cttagcctga
      301 ccgcatcctg gatgccgctg gaatttgacc ctgccgataa tgacgatcat gccatgcaac
      361 aagttgataa gcgtgatagt acggctatgg cgggggttgc ctggtatcac cacgagcgtt
      421 ggggaaccgt gaaagcctct gcagctgcgg acgttctgga taacagcaac ggctgggtgg
      481 gggagctatc ggttttccac aaaatgcaga taggtcgcct gtcgctgaca cctgcgctgg
      541 gcgttctcta ttatgacgag aatttcagtg actattacta tggcatttca gagagtgagt
      601 cccgtcgtag cggtctggca agttattccg cgcaggatgc ctgggtgccc tatgtcagcc
      661 tgacggcaaa atacccgata ggagagcacg tagtattgat ggcgagcgcc ggatacagcg
      721 agctgccgga agagattacc gacagcccga tgattgatcg taatgagagt tttacctttg
      781 tcaccggcgt gagctggcgt ttttaattca ccggtggatg tcggtgcggc tggtgctccg
      841 tcgtaatgcc acaaaggttg ttgctctgga ttgctctgtt cataccggga acctggcgtc
      901 tgaatcagga tcttatggct gaacaatccc ttgccttcaa atgtcgtaaa tggcgcatcg
      961 gcgggcagca acaggttatg cagtgactcg acgttgtcgg cctgctccat gcaatacggt
     1021 ttaatgaggt tggctatttc cggcagattt ttcgcaagca caccggttta acgccagcag
     1081 cgtggaagcg gcgttacagc aaggaacata tcaattcgta gtgccggggc gatgaagccc
     1141 cggcgtgagg gattactgcc cgtaataggc attggcccca tgcttacgca ggtagtgctt
     1201 atccagcagt tcgttttgca tcgcagggag ctgcggcgca agctggcgcg agaataggcc
     1261 catataggcg cattcttcga gtactacggc gttatgcacg gcatcggcgg cgtttttacc
     1321 ccatgcgaac gggccgtgag aatgcaccag caccgccggg atttgtgccg gactcctgcc
     1381 acgttcttcg aaggtttcaa tgatcacttc gccggtctga tattcatatt cgccgttaat
     1441 ctcctctgcg gtcatctgtc gcgtgcaggg gatggcacca tagaaataat cagcgtgcgt
     1501 ggtgccccag gcggggagat ccagcccggc ctgcgaccag atggtggcgt ggcgtgagtg
     1561 ggtatgcaca atgccaccga tttcggcgta gcgacggtaa agcgccagat gcgttggcgt
     1621 gtcggacgag ggctttttgc taccttccac caccttgccg ctggctatct caaccaccac
     1681 catatcgtcg gcggtcatca cgtcatactc gacgccggaa ggtttgatta ccatccattg
     1741 ccgcgtttcg tctaccgcgc tgacattgcc ccaggtgaac gtcaccagat ggtgagcggg
     1801 aagcgccaga ttcgccgcca gcacgtcggc tttcagttgc tctaacatat aaatccagcc
     1861 tcctgcatac gcgcttcaat ccaacgccgc gcctgaataa tctccagcac cggctctttg
     1921 gctttttcgg tccacatctc aatcaggaaa gaaccgcgat agttcagttt atgcagcgtt
     1981 ttgaagatgc caacgaaatc gacgcagcct tcgccaaacg gcacgtcgcg gaactgtccg
     2041 ggactttgct cggtaactgg ctgggtgtct ttcaggtgga tcgcggcgat acggtcaatg
     2101 cccagtttca gttcggcggg aacatcattg ccccaggcgc tgaggttgcc gacgtccggg
     2161 tagacggtga accacggcga ggcgagcatc tcgtcccact ttttccattt actgatggag
     2221 ttcataaacg cggtgtccat catctccacc gctagcatga cttgtgatgc cgccgcctgt
     2281 tcgaccgccc acgccagccc ttcagcaaaa cgttgccggg tgccttcatc gtggtcttcg
     2341 taatagacgt cgtagcccgc cagctggatg gtgcgtatgc cgagatcgcg tgccagacga
     2401 atcgctttgc tcatgatctc ccgtgctcgc tggcgtaccg cgtcgtcacg gctaccaaaa
     2461 ggaaagcgac gatgtgcgga caggcacatc gacgggatcc caacacccgt ttcgatcatc
     2521 gcggcaacca gcgaagtcct ttgtgcggtg ctccagtcaa gacgtgaaag ccgttcgtcg
     2581 gtttcatcca ccgacatttc gacaaaatca aaaccgcagc tttttgccag caccagccgc
     2641 tccggccagg agagatcttt cgccagcgcc ttttcataaa tccctaacgg atgattacgc
     2701 acgcttgcct ccccagatag cgtcgatttg cgcatggaaa tcgccagcca cctgcgccgg
     2761 atttgctgag cctgccagtg ctcgcccggc aataaacgct ttcacgcgaa tatctttaaa
     2821 cagcggcagg tcagcagggg taatcccgcc ggtaatggaa agctcaaggc cgatatcgga
     2881 aagcgccttc atgcgtgcca gatcggcttc gccccactgt tgcccgctgg cctgtgcatc
     2941 acgaccgcga tgataaatcg cctgccgcac gccaatacga tgccagtcgc gggcgtcgtc
     3001 cagcgtccag ttaccgaaca gctcgatctg aatttcacca ccgcagcgtt gcgccatcgc
     3061 gtggcctttt tccaccgtcg cgagcggtgc ggcgcagatg atggtcatcc agttagcgcc
     3121 tgcgccaaat gcctgttgcg cgagcgtttc accagcgtcg gcgaccttcc agtcggcaac
     3181 gatgattttg tcagggcact gttcgcgcaa ggctttcacc gcgccaagcc cttcgtttaa
     3241 acagagaatg gtgcccgctt cgacaatatc gacgctgtct tttaactgcg tcacgtcgcg
     3301 ctgtgcggct tcaagtgatg agtggtcgag tgccagttgc agaagtggtc ggctcataat
     3361 atgtgctcct tgatgcgggc gtgaaagccc tgaagtgcgg caatgagatg ctgatagcgt
     3421 tggtattttt gttggtaaag ctgatgggcg gtcatatccg gcagcagggt gcgtaccggg
     3481 tgctgtaagt cacgttgagc ttcgctgaaa tcgcgataaa ctccggtgcc gacacgggcg
     3541 gcaagtgccg cgccaaaaca gccggtttct tccacctgcg gcagctcgat acgcagaccg
     3601 ctgacgtccg ccagcatttg catccagaca tcggagtgcg ccgggccgcc agtgacgcgc
     3661 agggtgtgca catcagtaaa acgttcgcgc atccggttga ggtgggtcat atggctgaac
     3721 accccccctt cataaatggc ctgcaacagg tgtgcgcggg tgtgaatggc ctgcatcccg
     3781 tagaaaccgc tggtcatctc gagtccggcg ttgctgccgt acaggaacgg caggaaaaag
     3841 agatcgccac cggccttcgg caagctggca acggcctggt tgatctcatc aaacgagatt
     3901 tctccccact gtgcggtaaa ccattcgagg ttgccggaag aggtggggct ggcttcgtga
     3961 acgataaatt caccatcgtt aacgtagcga ccatagacat acggatgcgc ttcaccgtca
     4021 cgtaaaccgc gggttatgcc gctggtcacc gcccaggtgc ccatcaccgc attaagggta
     4081 aattcgtctt cgatcccggc gcagagtgcg gtggaaacca catcaaacag gccgccgaca
     4141 acgggcgtac ccgctttcag accggtcagt acggctgtct gagcggtgat ctccccgcag
     4201 atttcggcag atccgacaac aggcggcagg gcgtgattga tttcagcaat ccccagccag
     4261 tcggtgaggc acgggtcata ttccccaaga ctcatgttgt agagattgga ctcggaaata
     4321 ttgctctctt cacagccttt gacgccggtt aaacaccagc gcaggtagtc gtgcgtcatc
     4381 atcacgcagc caatttgcgc gtagcgttcc ggttcgtgct ctttaagcca acgtaacagc
     4441 gataccggat gcccggtcca caaggtttgt cgggtcagcg ggtagagttt ttccgggatg
     4501 ccatcttcct gccagcgacg aacgatttcc atcgcccggc gatccgagga taaaatggcg
     4561 ttaccgagcg gcttgttgtt tttatccagc aaaaacaagc cttttccttg tgcggagata
     4621 ccgataccga caatttgttc cccgctaacg ccggaatggg taagcagttc gcgaatgaca
     4681 gccatgcagc attgccacag ttctgccata tcgcgctctg cccaacctgg ctgcgggctt
     4741 aatgcgcaca gcggcaggcg ctgcacgcct gcctcccggc cttcgcggtc atacagcccg
     4801 gctttcagcc agctaccgcc acaatctaac cccagccagt attgcgtcat ggtttattgc
     4861 acctcatcca cggctttcag tagatcagca ccctgcggaa catcttttac aaacatgtcg
     4921 cgaacctgat tacccagagc gtcgctaaag gctttgcggt cgagatcggt gatgacttcc
     4981 acgcccgctt ctttcatgcc gtcgatgatt ttttgctgat cttcggcaac cagtttgcgc
     5041 tggtagttac ccgcttcctg cgcagatgaa attagcgctt gctggaactc cgggcttaag
     5101 ccatcaaact tcgctttgtt gatcaccacc agaagcgggg aataggcgtg gtgcgtcaga
     5161 gaaaggtact tctgcacttc gaaaaatttt gctgaccaga cgacgttgat cgggtgttcc
     5221 tgagcgtcga tagtgcgggt ttccagcccg gtatagactt cggcaaacgg catcgggatc
     5281 gggttagcgc caaagacttt gaatgcggcg atattcatcg ggctattgtt ggtgcgaatt
     5341 ttcagccctt tcaggtcggc gggggtttta accggtgcgc gcgagttggt gacatcgcgc
     5401 cagccgtttt cccagtaggc cagtactttt agtcctttac cttcaagtga ggctttcaga
     5461 tcatcaccga ctttgccgtc gagcgtttta tgcgcgtgag cggtatcgcg gaacaggaaa
     5521 gggacatcaa gcaagttcat cactggtgat aacccggcaa agttattcga gccggacatt
     5581 tccatatcga tggtgccgcc acgtacgccg ctgatcatcg cctgcgcgtt accgagggtg
     5641 ctatccggaa acaattttaa tttcagctcg cctttggttc tctcctgcaa taaatcattg
     5701 aattttttcg ccgcaatatg ttgcgagtcg gtttgtgagg tttcataacc gaaacgtaaa
     5761 gattgcgccg ccagagaaga tgtgctgaat gcagccaggc cagcaatgaa taatgcgtag
     5821 gttacagagc gtaat
//
LOCUS       DAZFXT010000210         5759 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.210, whole genome shotgun sequence.
ACCESSION   DAZFXT010000210 DAZFXT010000000
VERSION     DAZFXT010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5759
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.210"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            75..1172
                     /locus_tag="ACHKW0_004715"
     CDS             75..1172
                     /locus_tag="ACHKW0_004715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HFO7477389.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRT
                     DTQVNAGKVLPEVFASGKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKA
                     QNFEAVAQYQFDFGLRPSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVD
                     YKINLLDKNDFTKALGVSTDDIVAVGLVYQF"
     gene            complement(1362..1745)
                     /gene="quuD"
                     /locus_tag="ACHKW0_004716"
     CDS             complement(1362..1745)
                     /gene="quuD"
                     /locus_tag="ACHKW0_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HFO7477390.1"
                     /translation="MCDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(1763..2752)
                     /locus_tag="ACHKW0_004717"
     CDS             complement(1763..2752)
                     /locus_tag="ACHKW0_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7477391.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            complement(2760..3569)
                     /locus_tag="ACHKW0_004718"
     CDS             complement(2760..3569)
                     /locus_tag="ACHKW0_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO7477392.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSAPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            complement(3589..3978)
                     /locus_tag="ACHKW0_004719"
     CDS             complement(3589..3978)
                     /locus_tag="ACHKW0_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7477393.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPKGKVEITISKYEKTAGAAA"
     gene            complement(3975..4301)
                     /locus_tag="ACHKW0_004720"
     CDS             complement(3975..4301)
                     /locus_tag="ACHKW0_004720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO7477394.1"
                     /translation="MTTLTQCQQQVLDMLISYQQERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRAAHWLH
                     ERGLKV"
     gene            complement(4298..4951)
                     /locus_tag="ACHKW0_004721"
     CDS             complement(4298..4951)
                     /locus_tag="ACHKW0_004721"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7477395.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            complement(4951..5445)
                     /locus_tag="ACHKW0_004722"
     CDS             complement(4951..5445)
                     /locus_tag="ACHKW0_004722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7477396.1"
                     /translation="MIMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            complement(5442..>5759)
                     /locus_tag="ACHKW0_004723"
     CDS             complement(5442..>5759)
                     /locus_tag="ACHKW0_004723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfO"
                     /protein_id="HFO7477397.1"
                     /translation="AAECDGEVVRPKEPNWTAWANEIRLMCVQDGRTHKQICEMYSRV
                     SRDPFWCRNVLSPSKLREKWDELSLRLSPSVSTYTEKREDPYFKSSYDNVDYSQIPAG
                     FRG"
BASE COUNT         1391 a         1595 c         1336 g         1437 t
ORIGIN      
        1 tcacattgat tagatgaata tttatcgcgc agtgacatca ttttttaata atagttcaaa
       61 aaaaagggct cacgatgaaa aaattaacag tggcaatttc tgctgtagct gcatcagtac
      121 tgatggcgat gtctgctcag gcagctgaaa tttataataa agacagtaac aagctggatc
      181 tgtacgggaa agttaatgcc aagcactact tttcctctaa cgatgcagat gatggtgata
      241 ctacttatgt tcgtctgggc ttcaaaggcg aaacccaaat caacgatcag ctgactggtt
      301 tcggtcagtg ggaatatgaa ttcaaaggca accgcgctga atctcaaggt tcctccaaag
      361 acaaaacccg tcttgcattt gcaggcctga aattcggtga ctacggctca atcgattacg
      421 gccgtaacta cggtgtagca tacgacatcg gtgcgtggac tgacgttctg ccagaattcg
      481 gtggcgatac ctggacccaa acagatgtgt tcatgactgg tcgcaccact ggtgttgcaa
      541 cctatcgtaa caacgacttc tttggtctgg ttgatggtct gaactttgct gctcagtacc
      601 aaggcaaaaa cgatcgtagc gatttcgata actacaccga aggtaacggt gatggcttcg
      661 gtttctctgc tacctatgaa tacgaaggat tcggtatcgg tgcaacttat gcgaaatctg
      721 atcgtaccga cactcaagtt aatgcaggga aagttcttcc tgaagtattt gcttccggta
      781 aaaatgcaga agtttgggcc gcaggtctga aatatgacgc taacaacatt tacctggcca
      841 ctacctattc tgaaacccag aatatgactg tatttgctga tcacttcgtt gctaataaag
      901 ctcaaaactt cgaagctgtt gcacaatatc agttcgattt cggtctgcgt ccgtccgttg
      961 cttacctgca atctaaaggt aaggatcttg gagtatgggg cgatcaggac ttagtcaaat
     1021 atgttgatgt aggtgcaacc tattacttca acaaaaatat gtctactttc gttgattaca
     1081 aaatcaacct gcttgacaaa aatgacttca ctaaagcact cggtgtaagc actgatgaca
     1141 tcgttgctgt aggtctggtt taccagttct aatctgatta cgaaaaagat atgttgcggg
     1201 aggcgttgcc tccccaacat ataagtggct ccctcaagcc acttccttta ggagcacaac
     1261 cttgcttcta actatataaa ccttctgtta tatattaccc tttatttttg ggggcgttgc
     1321 aacgccccat ttttaataac tttcagtaaa caattggcat attaattaga gttattaaca
     1381 acgatatcca tctctaaccg gatatctaat gccattaaca tcccttcaat tatgccctca
     1441 gccttctgta accttttccc gatataacca tcagagcagc aatgcttacc tgccagtgac
     1501 atgaatgtca taccgactac ataataatct actaataaat cgtgcaaatc gctgttgttc
     1561 tttttcagac gggccatgca cccgcaaatg atcatcgcgt catcgtcaca acattgcggg
     1621 cgagatttta cttttgaagg aattaatccc ttaaaaccgg cggcaatgga cgaccaggtc
     1681 acatcttcat gattattagc cgcccacgct ccccaacgtt caagaaccat ctgaatatca
     1741 cacattaact ttctccacaa aatcaggcca gcacaccaat cgccagtgcg cgatcgataa
     1801 aacgaaatat cagctccagc tgggagccat acttctcttc aaatgccacg gtatccgcat
     1861 gcagttcgtc gtggtgtttt ctgcacaaag gcagcacaaa aaggtcatgc gcttttgtac
     1921 ccattccacc ctgaccatgg cctatcagat gatgcggatc atcagctggc tttccacaac
     1981 atgcacacgg ctgtgtctta acccagcgcg tgtacttttc attaacccag cggcgacgtt
     2041 ttgggcgtaa cataaaagac tccggcgact ccggatccac tttcagcgcc agcacctttt
     2101 tcgccttatc ctggatgatg ctggtggcag gaaccgaagg cacaaggtca ctttcccggg
     2161 taacagacgg cacaacaggc ttcggtaatc tcagtgcctt acgggctgca ctttccggta
     2221 aggcatccgc cagatcatta cgaatcagcc accagcacag ttccggcatt gtcacaacgt
     2281 gactgtcatc aaaaccgaga tcccgacgca caacagacaa cacccagcgg gcacagttat
     2341 ccgttgccat tgattccagc cgttccgtga actgatcgcg cagctggtta tcgcagtgcc
     2401 agcacagacg gattgcaccc ggagcgtgtc gcattgtggt catgttctcg ctgtgccagt
     2461 cggaatgagg ccactggcag cctttttcac gaagtaacca gctttcaaga cattccacgc
     2521 caccagcacg acggatcact gcctcattgc ggaacacggc ccgaacggca ggatcatccg
     2581 ccagcggttg tgatgccgcc ggaacggcac cactggcgaa agatgaataa cgctccggct
     2641 caggctccag caggacacgc ccctgcataa acaggggcat cagctctgaa cctggcctga
     2701 acaatacgat ccccatacgc ggggcaattt caggggtcag tagtgctctc acggtcacct
     2761 caatgaacgg tatcgagcag ctttaacagc tcagggaatc gggattcgaa gaaatgcggc
     2821 tgcgtctcgc gcggatttgc gggactggtg atgttcttgc cgaacatgca acctttcgct
     2881 gtcagcgacc agaatttttt gatgttgtta atcgctgtac ggctgtatcg ttcgcgctgc
     2941 tcgacgatcc ccagtttcac catctggtga tatgcctgat tagccgtcag gcgtatacca
     3001 tactgtttca gcagtgcact cagtgacagt gtcgggcgac ttgagccatc gtgtgcatca
     3061 gcaggagcat caatggcata gcgcggtgcc agattcggta agccaacagc ctcctggagt
     3121 ttctgacagg caccaagcac tgaagagtta gacaggttta actcccggcg cataaagtcc
     3181 agcaggatca cgccagcctg catcttgtca gcagcctgtc cggataattt ttccggtgcg
     3241 ctggttacca tatcgaaagt acggatcacc ttcagatgga atgacgggct gatccacatt
     3301 gcataggcat acaccagttc cttgcagaca tacgttcccc gttcatttcc cccatgaatc
     3361 acactcaccg ggtcaacacc caaattctgg gtgttggtca attcatgaac aagctcaaca
     3421 gtttgttggc tggaaagaaa ctttcccggc tccttggttc tggcatttgc accagatgct
     3481 actgctgcgc gatgcagatc gttcaggctg taacgcccat aagcatcacg acgaacttca
     3541 ataccatcaa tgaccatcag attattcata cttcgtttct cctcttgatc aggcggctgc
     3601 acccgccgtt ttctcgtact tactgatagt gatctcgacc ttccctttcg ggataaccgg
     3661 tccccactcc accagcattc ttttcacctg actgtcgtct tcccacacac ccgcatgggt
     3721 cagggcgtca aacagcgcct tgttatagtt gtccagatcg cggatccggt tatccggagg
     3781 aaacaacacg atctccactg aagcaggtgc cgacgttggt ttcggcagac gacgtaactg
     3841 ctcaactatt gctgcgcacg ccgcgctctg gaatttgcgc cccgctgcgc ttatcagact
     3901 cttaccagca aacgcccctt tgttggggtg tcgccagtac gtgttcacgc tgggagggaa
     3961 aggcaagatc agcttcatac tttcaggccc ctctcatgta accagtgggc tgcacgcagc
     4021 ctggcgtttt cctcaccggc aagcagtgcg cggataatcc cgaccgcctc gctgtcgtcg
     4081 tccttcaccg cggtatgaag agttatcccc cgggccacgc cacgctttat cgtgatgacg
     4141 ccttttttct ccagtgcgcg aagatgctcc accgctgcat tcaccgaacg gtatcccagc
     4201 atggttgcca cctcctgatt ggttggcgga aagccacgtt cttgctggta agaaatcagc
     4261 atatccagca cctgctgctg gcattgagtt aacgtcgtca tgccgccatc tccctgacca
     4321 gtttttccgc ctgctggcga acctgcgcca gaaaggcctc accacatgcc tcaagttcat
     4381 cgcgcccgat gtagctgatt gccggtccct tccaggtctt gtcgaaaaca gcaatagcac
     4441 cagcgaagaa agcgcctgtc ggcacctgct tctcatcctt cgggataaac caggcaggca
     4501 gttcaaaacc aatacgcccg cgaataaaag caatatggtc cgcatcttcc ggccaccaca
     4561 cttcgctggt ggcagctttg atcaggaaaa catagcgccc gcccttatca cgcatggcac
     4621 tggcatgttt catgatgtaa cgcatgccgg tgatgtattg cccctcatgc tgactggcgc
     4681 ggctgtatgg gggattacca aaggcagcac ctttaagctc cgcaagacgt tctgaccagt
     4741 catgcgccag cgcgttatct tccgccgtgt aatacgcggc acatttggcg ttatcaccgt
     4801 cagtaaacag atccagaaca aacgggccaa acagggtgtt aattccccag aaaatgttgt
     4861 ccggcgtgcg ccactgatcg cccacttcct tcagttcatg ggctggtttg ttccgcagtt
     4921 ccaccagcgc ctggcaatat ttattactca ttaagccccc acgtaattcc ctgacagata
     4981 ccactcttca cccgatgcag cgcgcttgct gcttttccgt aagcaccgct cacgacgcgc
     5041 cagaaaattg tttcgttctg gctgggagtg gctttcacgg aatgccgcca tccacaccgt
     5101 tgcagcacga cggtataagc ccctggactc cagttcttca gcctggcggg tcaggcacaa
     5161 aatcacccgt ggatcgttag tgccgacata gaaattgcgc acaggtctgg tttctcgaac
     5221 tggttgtggt tccggttcct gcgctctctc agtcaggcgc gggaaatgtc tgcgtgtatc
     5281 tccttcacaa cggtgagcca cacgcccact ctgacgtaac ttgcttgctg actgcagaac
     5341 gcgctgccgt gagtaacctg caaaagcatc cgcaatgtct ccggaagtac atcccggatg
     5401 ggcttcaatg aatttctgaa cttcattcaa aagactcatg atcaccccct gaatcctgcc
     5461 gggatctggc tgtagtccac gttgtcgtaa ctggatttga agtacgggtc ttcgcgtttt
     5521 tcggtgtacg tgctgacgga cggtgataag cgcagggaaa gctcatccca tttttcccgc
     5581 aacttcgacg ggctgagcac gttacggcac cagaacggat cgcggctgac gcggctgtac
     5641 atctcgcaga tttgtttgtg agtacgacca tcctgcacac acatcaggcg aatttcgttt
     5701 gcccaggctg tccagttcgg ttctttggga cgaaccacct cgccgtcaca ttcggcggc
//
LOCUS       DAZFXT010000211         5409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.211, whole genome shotgun sequence.
ACCESSION   DAZFXT010000211 DAZFXT010000000
VERSION     DAZFXT010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.211"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(147..491)
                     /locus_tag="ACHKW0_004724"
     CDS             complement(147..491)
                     /locus_tag="ACHKW0_004724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477398.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGAPH
                     DAADFRRCVALVEQIPEIRDSFNKVAKRVPAFKGILNEWDSLVALLKSEMKIHGNKAP
                     ETYRRISELRKD"
     gene            complement(520..687)
                     /locus_tag="ACHKW0_004725"
     CDS             complement(520..687)
                     /locus_tag="ACHKW0_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477399.1"
                     /translation="MHFRVTGELNGEPFNRVIEAEDINDCYDHWMIWAQIAHADVTNI
                     RIEELKEHQAA"
     gene            complement(723..1022)
                     /locus_tag="ACHKW0_004726"
     CDS             complement(723..1022)
                     /locus_tag="ACHKW0_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477400.1"
                     /translation="MACSTFNPLTLQKYQPDPEDLCSLCGGNHGKAAMIECKDKIHIC
                     LNCVDVLVDIKNEREDKKRSEAIRALDSWMRDGYSAAQIYDLAISKGEIPGVRIE"
     gene            complement(1180..1458)
                     /locus_tag="ACHKW0_004727"
     CDS             complement(1180..1458)
                     /locus_tag="ACHKW0_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4752 family protein"
                     /protein_id="HFO7477401.1"
                     /translation="MDSFAKYTIIDWIAFLQVLLIWFYMAYRSGQWIVSVACSKGWRW
                     WNRKNKKALALDSFYEAFNLNSLQPGYVIVVTTQSGMTIQIHKPKEEK"
     gene            complement(1458..2141)
                     /locus_tag="ACHKW0_004728"
     CDS             complement(1458..2141)
                     /locus_tag="ACHKW0_004728"
                     /inference="COORDINATES: protein motif:HMM:NF016342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dATP/dGTP pyrophosphohydrolase domain-containing
                     protein"
                     /protein_id="HFO7477402.1"
                     /translation="MASLERELIRHEHAKWSDSTFGCVGPIGPLKHLSKEALEAAAEP
                     DDLSEWADMHFLLWDAQRRAGISDAEITAAMEDKLKINMERQWPEPKDGEPRLHIKEP
                     GNSPVIPDGLSTVCAEAYQVVGVMADALGVFGDAAVQKVLDNLSQQKLVHRDVLPFSL
                     PVTPDGWISCSELMPDKLIPVMVMYEDGEMWSAMWNGNRWDDGTEYPDPHSVTHWREM
                     PAAPQQEVK"
     gene            complement(2115..2240)
                     /locus_tag="ACHKW0_004729"
                     /pseudo
     CDS             complement(2115..2240)
                     /locus_tag="ACHKW0_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309978.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF550 domain-containing protein"
     gene            complement(2251..3006)
                     /locus_tag="ACHKW0_004730"
     CDS             complement(2251..3006)
                     /locus_tag="ACHKW0_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477403.1"
                     /translation="MIHYHGGPITPDTCAMRAWKGRHAFISFAHSGQINLAAEYCQSF
                     ALDNGAFTAWKAAGKNKIDWSDYYEFVARWKNHPGFDFAIIPDVIDGGEEENDALLNE
                     WPHGKLAGVPVWHVNESDERFIHLCNEFPRVAIGSCGDYDVKRPTLAVARMKDLIRHI
                     VDGHGQPVTKLHGLRMLNPLIFTKLPLASADSTNVARNIGIDKAWSGAYAPASKETRA
                     ALMVERIEAHNSPGSLAYCEQRDRFEMQLQLAL"
     gene            complement(3008..3415)
                     /locus_tag="ACHKW0_004731"
     CDS             complement(3008..3415)
                     /locus_tag="ACHKW0_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7477404.1"
                     /translation="MSEINSQALREAAEKAGEDKWQAKKINGDFFVIRHGSYTRQHGY
                     TSYQPIAEIDCKPVRDFVAKANPATVLELLDELEAAKKRIAELEAREILLPERSSMLH
                     RTDFHDDYQTVMAYKVSEVIDAIRATGIRIKGE"
     gene            complement(3412..3633)
                     /locus_tag="ACHKW0_004732"
     CDS             complement(3412..3633)
                     /locus_tag="ACHKW0_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7477405.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNYQTISATHCCECGDPIDE
                     RRRLVVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(3732..3947)
                     /locus_tag="ACHKW0_004733"
     CDS             complement(3732..3947)
                     /locus_tag="ACHKW0_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7477406.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            complement(4024..4215)
                     /locus_tag="ACHKW0_004734"
     CDS             complement(4024..4215)
                     /locus_tag="ACHKW0_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO7477407.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLAASL
                     SQKLEMMAAKAEANERDPA"
     gene            complement(4188..4370)
                     /locus_tag="ACHKW0_004735"
     CDS             complement(4188..4370)
                     /locus_tag="ACHKW0_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO7477408.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPGLSVIRNPLKAQRLAEE
                     INNKRGAVCTKHLLLN"
     gene            complement(4367..5047)
                     /locus_tag="ACHKW0_004736"
     CDS             complement(4367..5047)
                     /locus_tag="ACHKW0_004736"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7477409.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKP
                     RSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARALFEFTSGVNVTE
                     SPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQY
                     SMWVTRKDAWYFANYDPRMKREGLHYVVIERDENYMASFDEMVPEFIEKMDEALAEIG
                     FVFGEQWR"
     gene            complement(5044..>5409)
                     /locus_tag="ACHKW0_004737"
     CDS             complement(5044..>5409)
                     /locus_tag="ACHKW0_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO7477410.1"
                     /translation="GPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAY
                     TAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAE
                     AVKALGFLKQKATEQKVAA"
BASE COUNT         1284 a         1393 c         1202 g         1530 t
ORIGIN      
        1 gcgttccact cctgaagtgt caagtacatc gcaaagtctc cgcaattaca cgcaagaaaa
       61 agccgcattg atgcggcgat ggtaggtctg gatatcattg agcaatgaac aggcctcacc
      121 gagtgtgagg cggtgttatt tcatggttag tccttgcgta gctcgctgat tcttctgtaa
      181 gtctctggtg ctttgtttcc gtgtatcttc atttcagact tcaacagagc aacgagggaa
      241 tcccattcgt tgaggattcc tttgaatgcc ggaacgcgct ttgcaacctt gttgaatgaa
      301 tctctgattt ctggaatctg ctcaacaagt gcaacgcatc gtctgaaatc ggctgcgtca
      361 tggggagcgc cgaagtgatg accatagata ttctttttca gtccacatgc gattgaggca
      421 agagttgcgc tactgatgcc aacatcgcca gtcgattgcc atttcaaaac cttcatagcc
      481 aaatctgaca tttcttgtct ccaataaaaa aaaccgccat caggcggctt ggtgttcttt
      541 cagttcttca attcgaatat tggttacgtc tgcatgtgct atctgcgccc atatcatcca
      601 gtggtcatag cagtcgttga tgtcctctgc ttcgataact ctgttgaatg gttctccatt
      661 caattcacct gtgactcgga agtgcatttg tgatttcccc aaaagatgct tgagtgcgct
      721 tcttattcga ttcgcacacc tggtatttcg ccttttgaaa tggctaagtc ataaatttgc
      781 gcagcactat acccatctcg catccatgaa tctaaggcgc gaatagcctc gctacgcttt
      841 ttatcttctc tttcattttt gatatcaacg aggacatcaa cgcaattaag gcatatgtgg
      901 attttgtcct tacattcgat catggcagct ttaccatgat ttccgccaca cagtgagcat
      961 aaatcttcag ggtctggctg gtatttctgt aacgttaaag ggttgaatgt tgaacaggcc
     1021 ataatcatct ccataaaaca aaactcgccg tagcgagttc agataaaaga aatccccgtc
     1081 agtgcgagga ttgttattgt cttttcttcg tgcatttgtc gcactttcgg caccatccag
     1141 ataggcacat ccgtccgcaa ttaacacata taggccacat catttttcct cttttggttt
     1201 atgaatctga atggtcatgc cgctttgagt ggtgactaca atgacataac caggctgaag
     1261 actgttaaga ttgaatgctt cgtaaaacga atccaaggcc agcgcttttt tattctttcg
     1321 gttccaccaa cgccatccct tgctacaggc tacactgaca atccactgcc cactcctgta
     1381 agccatataa aaccagatga gcaaaacctg aaggaatgct atccagtcaa taatcgtata
     1441 tttcgcgaag gagtccatca cttcacctcc tgctgcggtg ctgctggcat ttcacgccag
     1501 tgcgtaactg agtgcggatc cggatattcg gtgccatcat cccagcgatt gccattccac
     1561 attgcagacc acatctcacc gtcttcatac atgaccatta ccggaattaa cttatccggc
     1621 attagctcac tacagcttat ccaaccatcc ggagttaccg gaagcgagaa cggcagcaca
     1681 tctctgtgaa caagtttttg ctgtgacagg ttatccagaa ctttctgtac tgctgcatca
     1741 ccgaatacac caagcgcatc tgccataact cctacaacct gataagcctc agcgcatacc
     1801 gtggataaac catccggaat taccggagag ttgccgggtt ctttaatgtg caagcgaggc
     1861 tcaccatctt ttggttcagg ccactggcgc tccatgttga tcttcaattt atcttccata
     1921 gcagcggtaa tttcagcatc gctgatgcca gcacggcgct gtgcatccca caacaggaaa
     1981 tgcatatcag cccactcgct gagatcgtct ggttcggctg cggcttccag agcctctttt
     2041 gagaggtgtt tcagtggacc aatggggcca acgcagccaa atgtggagtc agaccatttg
     2101 gcatgctcgt ggcgaatcag ttcgcgttcc agtgatgcca gtgcaattcg tgccagttcc
     2161 atttgttcgc cacgagtaag cccgttttca agcggatttt taatgaacaa ttcaatacgt
     2221 tctttggtaa tagtggtcat ttgttagtcc ttaaagtgct agttgcaatt gcatttcaaa
     2281 gcggtcgcgt tgttcacaat acgcaagaga accagggcta ttgtgtgcct caatccgttc
     2341 taccattaat gctgcgcgtg tctctttact tgcaggtgca taagccccag accaggcttt
     2401 atcaataccg atgtttcgag cgacgttcgt actatctgcg ctggctaagg gtaattttgt
     2461 gaatatcagc ggatttaaca tgcgcaatcc atgtagtttc gtaaccggct gaccatgccc
     2521 atcaacaatg tgacgaatca ggtctttcat tctggctacc gcaagagttg ggcgctttac
     2581 gtcatagtcg ccacaactac cgatagccac tcgcggaaac tcattgcaca aatgaataaa
     2641 tcgctcgtca ctttcattca cgtgccacac tggaacgcca gctagttttc cgtgaggcca
     2701 ctcattcaga agcgcatcat tttcctcctc tccgccatca ataacatccg ggataatggc
     2761 aaaatcgaat cctgggtgat tcttccagcg agcaacaaac tcgtagtaat cgctccagtc
     2821 gattttgttt ttgccagctg ctttccaggc ggtgaatgca ccgttgtcca gcgcgaacga
     2881 ctgacagtat tcagccgcga gattgatctg gcctgaatgc gcaaaactga taaacgcatg
     2941 tcgccctttc catgctctca ttgcgcacgt atcaggagta ataggcccac cgtggtagtg
     3001 aatcatctca ctctcctttg atgcgaatgc cagtagcgcg gattgcatcg atgacttcag
     3061 aaactttgta tgccattacc gtttggtaat catcgtgaaa atctgttcga tgaagcatgc
     3121 tgctacgttc cgggagcagt atttcccgcg cttccagttc tgcaatgcgc ttttttgctg
     3181 cttccagttc atccagtaat tccagcacgg tagccggatt agccttggca acaaaatccc
     3241 ggactggctt acaatcaatc tccgcaatgg gttgatacga tgtgtagcca tgctgtcttg
     3301 tataactacc gtgacgaata acgaaaaaat caccatttat ttttttagcc tgccacttat
     3361 cttcaccggc tttctctgcc gcttcacgca gtgcctgaga gttaatttcg ctcacttcga
     3421 acctctctgt ttactgataa gctccagatc ctcctggcaa cttgcacaag tccgacaacc
     3481 ctgaacgacc aggcgtcttc gttcatctat gggatcgcca cactcacaac aatgagtggc
     3541 ggatatagtc tggtagttca gacgacgcat ttttattgct gtattgcgct gtaattcttc
     3601 aatttctgat gctgaatcaa tgatgtccgc catctttcat taatccctga attgttggtt
     3661 aatacgcttg agggtaaatg cgaataataa aaaaggagcc tgtagctccc tgatgatttt
     3721 gcttttcatg ttcaccgttc cttaaaaacg ccgtttaaca tgccgattgc caggcttaaa
     3781 tgagtcggtg tgaatcccat cagcgttacc gtttcgcggt gcttcttcag tacgctacgg
     3841 caaatgtcat cgacgttttt atccggaaac tgctgtctgg cttttttgat ttcagaatta
     3901 gcctgacggg caatgctgcg aagggcgttt tcttgctgag gtgtcattga acaagtccca
     3961 tgtcggcaag cataagcaca cagaatatga agcccgctgc cagaaaaatg cattctgttg
     4021 ttgtcatgcc gggtctctct cgtttgcttc tgctttcgcc gccatcattt ccagcttttg
     4081 tgaaagggat gcggctaacg tatgaaattc ttcgtctgtt tctactggta ttggcacaaa
     4141 cctgactcca atttgagcaa ggctatgtgc catctcgata ctcgttctta attcaacagg
     4201 agatgctttg tgcatacagc ccctcgttta ttatttatct cttcagccag ccgctgtgct
     4261 ttcagtggat ttcggataac agaaagaccg ggaaataccc agcctcgctt tgtaacggag
     4321 tagacgaaag tgatcgcgcc tacccggata ttatcgtgag gatgcttcat cgccattgct
     4381 ccccaaatac aaaaccaatt tcagccagtg cctcgtccat tttttcgatg aactccggca
     4441 ccatctcgtc aaaactcgcc atgtaatttt catcccgctc aatcacgaca taatgcaggc
     4501 cttcacgctt catgcgcggg tcatagttgg caaagtacca ggcatctttt cgcgtcaccc
     4561 acatgctgta ctgcacctgg gccatgtaag ccgactttat ggcctcgaaa ccaccgagcc
     4621 ggaacttcat gaaatcccgg gaggtaaacg ggcatttcag ttcaaggccg ttgccgtcac
     4681 tgcataaacc atcgggagag caggcggtgc gcatactttc gtcgcgatag atgatcgggg
     4741 attcagtaac attcacgccg gaagtgaact caaagagggc tctggcgtcg ttctcgtact
     4801 gttttcccca ggccagcgcc ttagcgttaa cttccggagc cacaccggtg caaacctcgg
     4861 caagcagggt gtggaagtag gacattttca tgtcaggcca tttctttccg gagcggggtt
     4921 ttgctatcac attgtgaacc tctgaagcgg tgatgacgcc gagccgtaat ttgtgccatg
     4981 catcatcccc ctgttcgaca gctctcacgt cgatcccggt acgctgcagg ataatgtccg
     5041 gtgtcatgct gccaccttct gctcagtggc tttctgtttc aggaatccaa gagctttcac
     5101 tgcttcggcc tgtgtcagtt ctgacgatgc tcgaatgtcg cggcgaaata tctgggaaca
     5161 gagcggcaat aagtcgtcat cccatgtttt atccagggca atcagcagag tgttaatctc
     5221 ctgcatggtt tcatcgttaa ccggagtgat gtcgcgttcc ggctgacgtt ctgcagtgta
     5281 tgcggtattt tcgacaatgc gctcggcttc atccttgtca tagataccag caaatccgaa
     5341 ggccagacgg gcacactgaa tcatggcttt atgccgtaac atccgtttgg gatgcgactg
     5401 ccacggtcc
//
LOCUS       DAZFXT010000212         5326 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.212, whole genome shotgun sequence.
ACCESSION   DAZFXT010000212 DAZFXT010000000
VERSION     DAZFXT010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5326
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.212"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..84)
                     /locus_tag="ACHKW0_004738"
     CDS             complement(<1..84)
                     /locus_tag="ACHKW0_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7477411.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGE"
     gene            complement(326..856)
                     /locus_tag="ACHKW0_004739"
     CDS             complement(326..856)
                     /locus_tag="ACHKW0_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO7477412.1"
                     /translation="MIYPEITGKSGEHLRLNTLEAVWIQGKLRMWGRWSYIGGGKSGN
                     MFNRLLVSKKLTKTAVNEVLRSMKKSGLEKPELEAFFRDMTRGKQKSWLSHCTDTEAL
                     IIDRVISEVLGEYPGLINILRQRYEGRGMSKRKMAECLNRTHPEWCFSTCEKRIAGWL
                     AVAEHMLYVPMHDSFR"
     gene            complement(865..1224)
                     /locus_tag="ACHKW0_004740"
     CDS             complement(865..1224)
                     /locus_tag="ACHKW0_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7477413.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVSGGRLGVKIYKIESE"
     gene            complement(1237..2286)
                     /locus_tag="ACHKW0_004741"
     CDS             complement(1237..2286)
                     /locus_tag="ACHKW0_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7477414.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMQVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSDERVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVCNQVADALPEAVLRRSLGLRVEKIRSLYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKAHDIFTLPLCREHHNELHADPLEFEKKYGSQIEL
                     IFRFLDHAFATGVLG"
     gene            complement(2288..2560)
                     /locus_tag="ACHKW0_004742"
     CDS             complement(2288..2560)
                     /locus_tag="ACHKW0_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477415.1"
                     /translation="MAHNTKHHNSDIAPASRQGRCSHTFKYDWYQHDPCTEEQAEWLI
                     HNYRRRGYEFKKALSLDYRHWIIYVRLLYSERPPRPSRTFQQRIWR"
     gene            2696..2953
                     /locus_tag="ACHKW0_004743"
     CDS             2696..2953
                     /locus_tag="ACHKW0_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HFO7477416.1"
                     /translation="MRTTQQFSITLTNEMADMVRARVASGAYASESEVIREGLRALNE
                     RDKAIEAWLTHSAAPSLDSIRENPNNGRSISQVRAAIRSGK"
     gene            2959..3258
                     /locus_tag="ACHKW0_004744"
     CDS             2959..3258
                     /locus_tag="ACHKW0_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HFO7477417.1"
                     /translation="MTYEVIITPEAEEQIINLHRYITEKAGNVIADNFANALLDYLDG
                     FSTFPHRGNKRDDIRQGMRVTHFRHRTIIAFAVDGRKVFIVGIYHGGQSYETDFL"
     gene            complement(3464..3808)
                     /locus_tag="ACHKW0_004745"
     CDS             complement(3464..3808)
                     /locus_tag="ACHKW0_004745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477418.1"
                     /translation="MTTITREQLIAHAEETIEAQRLCIPGTIDHDIIRTYKMDIAVLE
                     IALASLAAEPAGKLHEYKPVGYQRLVDELTMQVKQLAWQLRKAKPDCKLPDKAMDYLE
                     RNGLTSVEDVLR"
     gene            complement(4142..4320)
                     /locus_tag="ACHKW0_004746"
                     /pseudo
     CDS             complement(4142..4320)
                     /locus_tag="ACHKW0_004746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305741.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4322..5269)
                     /locus_tag="ACHKW0_004747"
     CDS             complement(4322..5269)
                     /locus_tag="ACHKW0_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ead/Ea22-like family protein"
                     /protein_id="HFO7477419.1"
                     /translation="MSEIDYQALREAAEKATKGCYIVGHTSGNQHGNITGVFVCQKWK
                     GEPGGVIAECHVNCLVETDAQAYANAEFIAAFNPNVALALLDERERNQQYIKRRDQEN
                     EDIALTVGKLRVELEAAKSKLNEQREYYEGVIADGSKRIAELEKQCAEWERKALSNFE
                     ECAAMAERIEEMQTKSAPDSFGIIGENIRTQDNRITSDPMFCVYQKREIVVDADYDHD
                     RIVWVDEDGNEANKRHSRRLELLHENFREPPEKWRRVAVKDIDEFVTCCFTEQGCKDY
                     LAVNGHNLRLPFIYVKSGFRNAEYIGIRNWLAGIRIKGE"
BASE COUNT         1251 a         1447 c         1157 g         1471 t
ORIGIN      
        1 ctccccgact gtcgccggtt cggtaacgct taattcatta aacaccactc tggcggtttc
       61 ggtcatatcc tgctgtttca gcatgtcttt ttcccttttt cggttaacgt gacacaccaa
      121 taactcttgt cgaaaaagcc agcaagctga aagaacggta ttaataacca ccagcgaatt
      181 tattgcgctg ctgtatatta cggacacaaa aaaaccacct tccggtggct tccttgtgcg
      241 aaaaaacttg catttcgcct cgcgatacag ctttgcgaag cttacaggaa ttcaagctgt
      301 ttctgcgtaa aaaagcaagc tttttttatc gaaatgaatc gtgcataggt acataaagca
      361 tgtgttcagc cacggctaac caacctgcaa tacgtttctc acatgtgctg aaacaccatt
      421 ccgggtgagt acgatttaaa cattctgcca tttttctctt actcattccc cgtccttcgt
      481 acctttgccg gagaatattg attagcccgg gatattcccc aagcacctca ctgataacgc
      541 gatcaataat caacgcctct gtgtctgtac aatgtgacaa ccagctcttc tgcttccctc
      601 tggtcatatc ccgaaaaaat gcctcaagtt ccggtttttc cagcccggat ttcttcatgc
      661 tgcgtaaaac ctcattaact gctgttttcg tcagcttttt cgaaaccagt aaccggttaa
      721 acatatttcc ggatttaccc ccaccgatat acgaccaccg cccccacatc cgtaatttcc
      781 cctggatcca gactgcttcc agcgtgttca ggcgtaaatg ttcgccgctt ttgcctgtaa
      841 tttccggata tatcatattt acgctcactc actctcaatt ttgtaaatct tcacacccag
      901 ccgtccacca gatactggct gaccgcgcac aatattgatt tcatcaaact gctcgtcatc
      961 aatgagcact tccgcatgcg tcagcgcatc cagcggtgct ttcagaatgt tgtccaggtc
     1021 acggcggcgc ttatccggtg gctctgcaat cacctttatc gccagccttc cggacaggct
     1081 taatttcagc cgctgctggc gaacaataag cgccactgcc cggcgataac gctccccggc
     1141 ttttgataca aaatatgtgc tgccacggcg tcgccagtaa gtgttcaccg tcggcgggta
     1201 aggtaaaacc aaatctatga gcatcagtca cctcttttac ccgagcacgc cggttgcaaa
     1261 ggcgtgatca agaaaacgaa aaattaactc aatctgagag ccgtactttt tctcaaactc
     1321 cagcgggtct gcatgaagtt cgttgtggtg ctcccggcac aacggtaacg tgaaaatatc
     1381 gtgggccttt gtccccattc ccccctgacc atgaccaatc aggtgatgcg gatcgtcagc
     1441 aggcttacca caacacgcac acggctgtgt ctttacccag cgcgtgtatt tctcgttaac
     1501 ccagcggcga cgtttaggtc gtttcatgaa agattccgga gactcaggat caacggcaat
     1561 gctgaccacc gtcttttcct gtggtgggtt ctgttgctgg tgggcgtgag gcagcggcgc
     1621 aagatttttt gtgcgctgct tcagtatgct ggtggcggtc tgctctcccg gtacgatgtc
     1681 gctttcgcgg tacaaggagc ggattttttc cacacgtaac cccagagaac gacgtaatac
     1741 ggcctccggt agcgcgtccg ccacctgatt gcagaccgcc caccaggata attcagccag
     1801 agataattca cgctcctgcg taccgcttat tgcgtgaccg atgacgtcaa tcatccatgc
     1861 tgacaggttt tgatgagcaa gttgctcgag tgattcggat gtctggtccc gtagctggtt
     1921 gtcgcagtgc cagcacaaca ccattgcgcc ggtaccataa cggtgaatga cagtttcgct
     1981 gtggtgataa tcgccgtgtg gccactggca ggatttaata tggcgcaaca gccagtcaga
     2041 caatgcacca gcaccaccag cagcacgaat cactcgttcg tcgctgaaaa atggcagcaa
     2101 tgttttgtct tccaccagcg gctggcgaac ggcaggaacg accccggacg gcagattacg
     2161 catgcttttc ggttccggct ccaccagtac ccgggtattg tgaaatacct gcatggattc
     2221 acggcccggc ttaacgatca ccagcccgag ttccggtacc agaacaggtc gaagtaatac
     2281 ccgcacgtta cctccagatg cgttgctgga atgtgcggga cggacgcggc gggcgttcgg
     2341 aataaaggag cctgacgtag attatccagt gacggtagtc gaggctaagg gctttcttaa
     2401 actcataccc acgtctgcgg tagttatgaa tcagccattc ggcctgttct tcagtgcagg
     2461 gatcgtgctg ataccagtca tatttgaatg tgtgagaaca ccgcccttgc ctgctggcag
     2521 gggcgatatc agaattgtga tgtttggtat tgtgcgccat cggttttctc tgctggcgca
     2581 gcaggtgcca gttgttcaag ctggcgtgcg gcaatattgt ctctgatttc tgttgtcgtc
     2641 aacaggcaac gtgctatcat cgaatggtgt tctatcctac tccgtgaggt ttaccatgcg
     2701 tacaacccaa caattcagca ttacattaac taacgaaatg gctgacatgg tgcgcgcccg
     2761 tgtggcttcc ggtgcctatg cttcagaaag cgaggtcatt cgtgaagggc ttcgcgcact
     2821 gaatgagcgc gataaagcaa tcgaagcgtg gttaacgcat tcagccgccc cctctcttga
     2881 ttctatccgc gaaaacccaa acaacggacg ctccatttca caggttcgcg ccgcgattcg
     2941 atccgggaag taatctgcat gacatatgaa gtcatcatta ctcctgaggc cgaagaacaa
     3001 ataatcaacc tgcacagata tataacggag aaagcaggga acgtcattgc tgacaatttt
     3061 gccaatgcgc ttcttgatta tcttgatggg ttttctacat tcccgcatcg gggcaataaa
     3121 cgcgatgata ttcgccaggg gatgcgggta actcatttcc gccacagaac gattattgct
     3181 tttgccgttg atggcagaaa agtctttatt gtcggtatct atcatggtgg gcaaagttat
     3241 gaaaccgatt tcttataaac ttttacccac atcattccgg tgttagaatt aaccgtccgc
     3301 ccccctctct tactggcgga ttcgtaggct atataaatca aagatcccgg ctcatgtttg
     3361 tgtcgggatc ttttttcggc gatttatccc cagcggcaaa tcgaatacac caccagcgcc
     3421 accgccatcg caattcctac cgttgtgaat gcttcaggcc tggtcatcgt aaaacatcct
     3481 ccacgcttgt cagtccgttt cgctccaggt agtccatcgc cttatccggc agtttgcagt
     3541 ccggcttcgc tttcctcagt tgccaggcta actgctttac ctgcatagtt aactcgtcga
     3601 ccagacgctg ataccccact ggtttgtatt catgcaattt accggctggc tctgctgcca
     3661 gcgatgccag tgcgatttcc agaacagcaa tatccatctt atatgtgcgg atgatgtcat
     3721 ggtcgattgt gcccggtata cacagtctct gtgcttcaat agtctcctct gcgtgagcta
     3781 ttaactgctc tctggtaata gtggtcatgc cgcgtttcct tctttcttat taacaattac
     3841 accgtcatat atttcattaa ggtgccctct caactccatg cgccttaatg cagacaacat
     3901 gtaatcgcat tcaacctgct tatttccagt aaatggctta tcgtcaggat taccccaaca
     3961 gcaattaccc ttgggccacc catgtacttt ccgtactctt ccgttaacaa cgtgaagtaa
     4021 tccccagcca ggaggtaaat cctcaattga aataattccc ggctcactaa taaagaatcg
     4081 ccagtcgccc attccaagag acggattttt acgaaaacgc ttttttctat ctgccaacaa
     4141 gtcagcacga gaacatttcg cctctatcag gcatgatgct gaatttctga atcccatagc
     4201 atctggctgt tctccggtac tggttacagc tataaagcgg tcatgaaaac aaaccttgaa
     4261 cccgttgcgc ttaaggaact tgtacgcaat ctgacagagt tcgcggtgtg ttaacgccat
     4321 atcactctcc tttgatgcga atgccagcaa gccagtttct tatgccgata tattcagcgt
     4381 tcctgaaacc gctttttaca tatataaatg gcaagcgaag attgtgacca ttgactgcca
     4441 ggtagtcttt acaaccctgt tcggtgaaac agcaggtaac gaattcatca atatctttca
     4501 cagcaacgcg ccgccatttt tctggtggct ctcgaaagtt ttcatgaagt agctcgagac
     4561 gacgactatg gcgtttattg gcttcattgc catcttcgtc aacccagaca atccggtcat
     4621 ggtcataatc agcatcaaca acgatttcgc gcttttgata cacacaaaac atgggatctg
     4681 acgttattcg attgtcctgt gttcgaatat tttcaccgat gatgccaaac gaatctggtg
     4741 cagattttgt ctgcatctct tcgatacgtt cagccatcgc agcacactct tcaaagttgc
     4801 ttaatgcttt tcgctcccat tcggcgcatt gtttttccag ttctgctatg cgcttacttc
     4861 catccgcgat tactccctcg taatattcac gctgctcgtt gagttttgat tttgctgctt
     4921 caagctcaac gcgcagcttc cctaccgtta gcgcaatatc ctcgttctcc tggtcgcggc
     4981 gtttgatgta ttgctggttt ctttcccgtt catccagaag cgccagcgca acatttggat
     5041 taaaggcagc aataaattca gcgtttgcgt aagcctgagc atctgtttca accaggcagt
     5101 taacatgaca ttctgcaatc acgccaccgg gttctccttt ccatttttga caaacaaaaa
     5161 ctcctgttat attcccatgc tgattgcccg atgtatgccc tacgatgtag catcctttag
     5221 ttgctttctc tgctgcttca cgcagcgcct gatagtcaat ctcgctcatt catcgcccca
     5281 ctcatcacaa tatgcttcga ccgcagtttt ccctgcttca taatca
//
LOCUS       DAZFXT010000213         5292 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.213, whole genome shotgun sequence.
ACCESSION   DAZFXT010000213 DAZFXT010000000
VERSION     DAZFXT010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.213"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..163
                     /locus_tag="ACHKW0_004748"
     CDS             <1..163
                     /locus_tag="ACHKW0_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477420.1"
                     /translation="EELLSDNTLEKDENSPDNGFEVGEYNTYEAYNSENQYFTREDYT
                     YNYDLLNAI"
     gene            complement(671..1047)
                     /gene="rnpB"
                     /locus_tag="ACHKW0_004749"
     ncRNA           complement(671..1047)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="ACHKW0_004749"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(1080..2225)
                     /gene="garK"
                     /locus_tag="ACHKW0_004750"
     CDS             complement(1080..2225)
                     /gene="garK"
                     /locus_tag="ACHKW0_004750"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HFO7477421.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYAEVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2322..3212)
                     /gene="garR"
                     /locus_tag="ACHKW0_004751"
     CDS             complement(2322..3212)
                     /gene="garR"
                     /locus_tag="ACHKW0_004751"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HFO7477422.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3242..4012)
                     /gene="garL"
                     /locus_tag="ACHKW0_004752"
     CDS             complement(3242..4012)
                     /gene="garL"
                     /locus_tag="ACHKW0_004752"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008672 - 2-dehydro-3-deoxyglucarate
                     aldolase activity [Evidence IEA];
                     GO_process: GO:0019394 - glucarate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HFO7477423.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(4028..5290)
                     /gene="garP"
                     /locus_tag="ACHKW0_004753"
     CDS             complement(4028..5290)
                     /gene="garP"
                     /locus_tag="ACHKW0_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005155249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HFO7477424.1"
                     /translation="MVTAVNYADRATLSIAGTEVAKELQLSAVSMGYIFSAFGWAHLL
                     MQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGFVDMFPLAWAGISMFFMRFMLGFSE
                     APSFPANARIVAAWFPTKERGTASAIFNSAQYFSLALFSPLLGWLTFAWGWEHVFTVM
                     GVIGFVLTALWIKLIHNPTDHPRMSAEELKFISENGAVVDMDHKKPGSAAASGPKLHY
                     IKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIYLVQEKGMSILKVGLVASIPALCGF
                     AGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLLASTIILCNYTNNTTLVVMLMALAF
                     FGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGNVASIVTPLVIGYLVSELHSFNAAL
                     VFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1292 a         1507 c         1261 g         1232 t
ORIGIN      
        1 agaagagtta ctttcagata acaccctcga gaaagacgaa aattctccgg acaatggttt
       61 tgaggtcggg gaatataaca catatgaagc atataactca gagaatcaat attttaccag
      121 agaggactat acgtataatt acgacctttt aaatgcaata tagacttaat aacttcttac
      181 aaagtctatt attaataccg tagatattta ttcatcttac cctgtgcacc aaaagcaaaa
      241 actcactgac gcgggtttga aactgatggc aaccgcaaaa atgcctgatg cgctacgctt
      301 atcaggccta cgccatctct gcaatatatt gaatttgcgt gcttttgtag gcaggataag
      361 gcgttcacgc cgcatccggc atgaataaag cgcactcaac aatctgaaac ccgccggaac
      421 ggtttattac gtacatcagg tgaaactgac cgataagccg ctttcttttg ggtatagcgt
      481 cgtggacagt cattcatctt tctgcccctc caaaagcaaa aacccgccgg tttacgctgg
      541 cgggttttcg ttaaatcagg tgaaactgac cgataagccg ctttcttttg ggtatagtgt
      601 cgtggacagt cattcatctt tctgcccctc caaaagcaaa aacccgccga agcgggtttt
      661 tacgtaaatc aggtgaaact gaccgataag ccgggttctg tcgtggacag tcattcatct
      721 aggccagcaa tcgctcactg gctcaagcag cctacccggg ttcagtacgg gccgtacctt
      781 atgaacccct atttggcctt gctccgggtg gagtttaccg tgccacggac tgttaccagc
      841 cgcgcggtgc gctcttaccg caccctttca cccttacctg atcccgcttg cgcgggccat
      901 cggcggtttg ctctctgttg cactggtcgt gggtttcccc cccaggcgtt acctggcacc
      961 ctgccctatg gagcccggac tttcctcccc tccgcccgtc tcccccgaag aggacgacga
     1021 cgaagcggcg actgtctggt cagcttcggc gcgcagtata gagggtttgc gcgcccttgt
     1081 caccccgcgt tgcgcattcc aatcgccagt gtcgcggcga tattacgtga agcacggcag
     1141 atattgtcat aagccccgcg gaatgcttcg tccaacgtac ctatgctggt caatacgctg
     1201 aagaccgcat cgatgccatg ctgatgtaca acgccaacat catcggtcag gctacccgca
     1261 atgccaatca ccggtttatg gtacttcttc gccacgtttg caacgccaat cggtaccttg
     1321 ccgtgaatac tctggctgtc aatacgccct tcaccggtga tcaccagcgt acaatcgtga
     1381 atatgttcct ccagattcag cgccgtagtg acgatttcaa taccactttt cagttccgca
     1441 ccaagaaacg ccatcagcgc cgcacccata ccgcccgcag ctcctgcgcc ggggacatct
     1501 ttcacatcaa catgcagcgc ttttttaatg acctcggcat agtgagagag gttattgtcc
     1561 agctcaacaa tcatcgcttc actggctccc ttttgtgggc caaagatgcg cgatgcgccg
     1621 ttatcgccca ccagcggatt ggtgacatca caagcgacgc gaatgacgca atcttttaag
     1681 cgcggatcga ggccggaaat atcaatatca ttcagagtat taagactacc gccgccaaaa
     1741 ccaatttcat tgccgttggc gtcgcataat ttcgccccca gcgcctgtac catgcctgcg
     1801 ccgccatcat ttgtagcgct accgccaatg ccgataataa tgtttgtcgc gccgctctcc
     1861 agcgcctgca ggattaactc gcctgtgccg cgtgaagtgg tcacgagtgg atcgcgtttt
     1921 tccgcaggta ccagctccag cccactggcc gccgccattt caataaacgc ggttttgcca
     1981 tcgccggaga tcccccaact ggcattcact ttctcgccca gcggccctgt aacccaggcg
     2041 tgacgttcag aaccctgggt ggctgcaatc atcgcttcca ccgttccttc gccaccgtcg
     2101 gcaaccggaa tagaaacgta ctgtgcatca ggaaaaattt cccgaaatcc tttttctatc
     2161 gcctgcgcaa cctcgctggc agataaactt tctttataag agtctggggc gattacgatt
     2221 ttcataccta tgcctgttac cacatgacgc cggagggcgt ttctcttatt cggcctggat
     2281 tccaggcccg gattgcaata cgccatccgg gcacgacgtc attaacgagt aacttcgact
     2341 ttcgccagtt tttcgtagta gcacgccagg gcgctatgat ccgccgttcc taaaccatct
     2401 gctcgcagtg cctgcatcat ctccataacc gcagctgtga gcggcagttg cgcgccgacg
     2461 ccgtgggaag tatccagcgc atttgccaga tccttaatat gcagatcaat acggaagccc
     2521 ggcttgaagt tgcggtccat caccatcggc gctttggcat ccagcacggt actgcccgcc
     2581 agtccaccgc gaattgcctg atacaccagg tccgggttaa cgcccgcttt ggttgccagc
     2641 gttagcgctt ctgacatcgc ggcaatattc agcgccacaa tgacctgatt tgccagtttg
     2701 gtgacgttac ctgcaccgat ttccccggta tgcaccacgg aacccgccat cgctttcatc
     2761 aaatcatagt atttgtcgaa aatagccttg tcgccgccca ccatcactga cagcgtaccg
     2821 tcgatggctt tcggttcacc gccgctcacc ggagcatcca gcatatcaat gcctttcgct
     2881 ttcagcgctt cgctgatttc acggcttgcc agcggtgcga tagaactcat atcgatcaat
     2941 accgtacctg gcttcgcgcc ttcaatgatg ccattctcac ccagcgccac ctctttcaca
     3001 tgaggggagt ttggcagcat ggttatgatg acgtcgcact gttcagcgat cgctttagcc
     3061 gtagacgctg tttctgcacc tgcagcaatc acgtcagcaa tagcttctgg gttacggtca
     3121 gcaaccacca gcgagtaacc tgctttcaga aggtttttac tcattggttt acccataatc
     3181 cccaggccaa taaaaccaac tttcatagtc atatcaatca tctctcttgt tgcggtggtg
     3241 gttatttttt aaaggtatca gccagtttct gagtggcaga gcggaagacg ccaagatcgc
     3301 tgccgacagc cacaaacgtc gcgccccatt ccagataacg acgcgcatcg gcttcgaccg
     3361 gcgcgagaat accgctgggt ttgccgtgcg cactggcacg gttaaaaatg tgctgaattg
     3421 ctttttgtac atccgggtgc gaggcattgc cgagatggcc taatgccgca gccagatcgc
     3481 tggggccgac gaagataccg tctacacctt cggtagcggc aatggcatcg acgttatcta
     3541 cgccctgctg actttctatc tggaccagaa tggtgatgtt cttgttcgac tgagcgaaat
     3601 aatccgccac ggtgccaaac atattggcgc ggtgagaaac ggagacgccg cgaatgcctt
     3661 ccggtgggta acgggttgat gccaccgcct gctctgcttc ctcttttgtt tctacaaaag
     3721 gaatcaggaa gttatagaaa ccgatatcca gaagacgctt aataattacc ggctcgttgg
     3781 tcggcactcg cactactggc gcgctggcgc tgcctttcag ggccattaac tgcggaataa
     3841 acgtggagat atcgtttggc gcatgttcgc catccagcac cagccagtca aacccagcca
     3901 aaccaagaac ttcagtgcta atcgggttag agagtgctga ccagcaacca atttgtacct
     3961 gtttcgcagc cagtgcggct ttgaatttat tcgggaaaac atcgttattc atcgcttata
     4021 cctttgctta tttctgcaat tccatacgtt taatgtcgcc aactacgaag aggtagcaga
     4081 ccatcgccat cagcgctgaa catcccacga aaaccagtgc tgcattgaag gagtgcagtt
     4141 cacttaccag gtagccaatc accagtggag tgacaatgga ggcaacattg ccaaagacgt
     4201 taaagacgcc gccacagagg ccaacaatct ctttcggcgc ggtgtcagaa atcaccggcc
     4261 agcccagagc accaaatcct ttgccaaaaa aagccagcgc cattagcatg accaccagcg
     4321 tggtgttgtt ggtgtagtta cataagatga tggtggaagc cagcaacatt cccagcacaa
     4381 tcggtagctt acgtgccagg gtcagggata aaccgcgttt gatcagataa tccgagaaga
     4441 cacctcccag cacgccgccc gcaaaaccac acagtgccgg aatcgaggcg accagaccca
     4501 ctttcagaat cgacatgcct ttttcctgca ccagataaat cgggaaccag gtgaggaaga
     4561 accaggtgat ggtgttgata aaatattgtc cgaaaaatac gcccaacatc atgcggttag
     4621 agagcaattg cttgatgtaa tgcagtttgg gaccgcttgc tgccgcactg cccggctttt
     4681 tgtggtccat atcgaccacc gcgccatttt cagagataaa cttcagctct tccgcagaca
     4741 tacgtgggtg atctgtcggg ttatgaatca atttgatcca cagcgccgtc agcacaaaac
     4801 caatcacccc cataacggta aagacgtgct cccagcccca ggcgaaagtc agccagccaa
     4861 gcagaggcga aaagagcgcc agcgagaaat attgcgccga gttaaagatg gcggaggcag
     4921 taccacgttc tttcgtcggg aaccaggcgg cgacaatacg ggcgttcgcc gggaatgatg
     4981 gcgcttccga gaagccgagc ataaagcgca taaagaacat ggagatccct gcccaggcca
     5041 gcgggaacat atcaacaaag ccttgcagga aagtgaatag cgaccagaaa aagaggctat
     5101 aggtataaac ttttttcgag ccaaacttat caagcagcca gccgccgggg atttgcatca
     5161 gcaagtgggc ccagccaaaa gcagaaaaga tgtaacccat cgaaaccgca cttaactgca
     5221 actcttttgc cacttcggta ccagcaatag acagcgttgc acgatcggcg tagttaacgg
     5281 cggtaacaat aa
//
LOCUS       DAZFXT010000214         5291 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.214, whole genome shotgun sequence.
ACCESSION   DAZFXT010000214 DAZFXT010000000
VERSION     DAZFXT010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.214"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            254..772
                     /locus_tag="ACHKW0_004754"
     CDS             254..772
                     /locus_tag="ACHKW0_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HFO7477425.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTDPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            982..3123
                     /locus_tag="ACHKW0_004755"
     CDS             982..3123
                     /locus_tag="ACHKW0_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HFO7477426.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFQQILDKMAYLTIWQGDDVQRRVKGMVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILGTILQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLDNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQESSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNQVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3199..>5291
                     /locus_tag="ACHKW0_004756"
     CDS             3199..>5291
                     /locus_tag="ACHKW0_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7477427.1"
                     /translation="MGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPNSPI
                     EEQKGNPVNPLLGAKVLPGETDLALPGPLPFILSRAYSSYRTRTPAPVGVFGPGWKAP
                     FDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSA
                     LWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDG
                     FGRTLAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGP
                     RSASSSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGEL
                     RAVYDRSGTQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRF
                     EYGESRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAA
                     GRRTEYSLHMASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDERGRLT
                     AETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYTTRYEY
                     DRYGQQIAVHREEGISTYSSYNPRGQLVSQKDAQGREIRYEYSAAGDLTATVSPDGKR
                     STIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLTEQRGF
                     DGRTQRYHYDLTRKLT"
BASE COUNT         1229 a         1367 c         1674 g         1021 t
ORIGIN      
        1 attttatgga ggcaatacgg actttgaaca gttgggtatg gttatccaga tatatattat
       61 taagttattg ctgaataaga gatttaatga ttttaaaaat agtttttacg acaaccgtaa
      121 attctagctc ggtttgtatg taaaaaatac tgaaaaatga tgcggttccg tgaagtgagc
      181 tgcatctgcc tgtctgggat taacggcagg tcattgtaag tagtaattaa ccacgttaac
      241 aaggagttta caaatgccaa caccgtgtta tatctccatc actggtcaga cccagggaaa
      301 tattactgca ggtgcattta ccgctgattc tgtcggcaac atctatgtgc aggggcacga
      361 agacgagatg ctggtgcagg aattcctgca taacgttact gtcccgaccg atcctcagtc
      421 cggtcagcct gcgggtcagc gtgctcacaa gcctttcatt tttaccgtgg cgctgaacaa
      481 agcggttccg ctgatgtaca acgcactggc ctccggcgaa atgctgccaa ccactgaact
      541 tcactggtgg cgtacctctg tggaaggcaa acaggagcat tatttcacta cccgcctgac
      601 tgattcgacg attgtcgata tgaaactgca tatgccgcat tgccaggacc cggcgaaacg
      661 cgagttcacc cagttgctcg aagtttcact ggcctaccgc aaaattgaat gggaacacgt
      721 caaatctggc acctccggtg ccgacgactg gcgcgcaccg ctggaagcat aagttaagcc
      781 aacagcatcc tgctatcagg ccggatgctg tttttgtatt acgccgtaga aagaaaggtt
      841 gtcagtaagg ggttacgttc acgggagatg gcggctgccg gaaacttcgg cggtagcgtg
      901 cctgaacgca agcgtaacac tgtactgtca cagccgcttt aacagcgaac tgtctgtttt
      961 tccttaaccc cggaggttgc tatgtcaacc ggattacgtt tcacgctgga agtggacggc
     1021 ctgccaccgg atgcttttgc ggtggtctcc tttcatctga accagtcact ctcttcgctt
     1081 ttttccctcg atctctctct ggtcagccag cagtttctct cccttgaatt ccagcagatc
     1141 ctcgacaaaa tggcctacct gacgatatgg cagggcgatg acgtacagcg ccgggtgaaa
     1201 ggtatggtga cctggtttga actgggggag aacgacaaaa accagatgct gtacagcatg
     1261 aaggtgtgcc caccgctgtg gcgcacaggg ctgcgccaga acttccgtat cttccagaat
     1321 gaggacatcg aaagcatcct cggcacgata ttgcaggaaa acggggtgac cgagtggagc
     1381 ccgctgttca gcgagccaca tccttcccgt gagttttgtg tccagtacgg tgagactgat
     1441 tacgatttcc tgtgccggat ggcggcggag gaaggcatct tcttttatga ggagcacgcg
     1501 caaaaaagta ccgaccagag cctggtcctg tgcgataccg tgcgttatct gccggagtcc
     1561 tttgagatcc cctggaatcc gaacacccgt accgaggtga gcaccctctg catcagccag
     1621 tttcgctaca gcgcacaaat ccgcccttct tccgtggtga ccaaagacta cacctttaaa
     1681 cgacccggct gggcagggcg ttttgatcag gaaggccagt accaggatta ccagcgcaca
     1741 cagtatgaag tgtatgacta ccccggacgt ttcaagggtg cccacgggca gaactttgcc
     1801 cgctggcaga tggatggctg gcgcaacaac gcagaagtgg cgcgcggaac aagccgttcg
     1861 ccggagatat ggccgggacg gcgaattgtg ctgacggggc atccgcaggc gaacctgaac
     1921 cgggaatggc aggtggtggc gagcgatctt cacggcgaac agccgcaggc ggtaccggga
     1981 cgcaggggtt caggcaccac gctggataac cactttgcgg taatcccggc agaccggaca
     2041 tggcggccac agccgttgct gaaaccgctg gtggacggcc cgcagagcgc cgtcgtgacg
     2101 ggaccggcag gcgaggaaat cttctgtgat gaacatggcc gcgtgcgggt gaaatttaac
     2161 tgggaccgct ataacccgtc aaaccaggaa agttcatgct ggatccgtgt ggcacaggcg
     2221 tgggcaggca ccggctttgg taacctggcg ataccgcgtg tgggtcagga ggtcattgtg
     2281 gacttcctca acggcgatcc ggaccagccg atcattatgg ggcgtaccta ccaccaggaa
     2341 aaccgcaccc ccggcagcct gccggggacg aagacgcaga tgacgatccg ctcgaaaacc
     2401 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
     2461 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cactgatgtg
     2521 ataaacaacc atgccgaaaa aataggtaac aaccaggcga tcaccgttac caataaccag
     2581 atccagaaca ttggcgttaa tcagatacag acggttggtg tcaaccaggt ggaaacggtg
     2641 ggcagtaacc agattatcaa agtgggatca aaccaggttg aaaaggtggg gatcattcgt
     2701 gcgctgacgg tgggtgtggc ttaccagacg acggtaggcg gcattatgaa tacctcggtg
     2761 gcgttgttgc agtcctcaca ggtagggctg cataaatcac tgatggtggg aatgggctac
     2821 agcgtcaatg tggggaataa cgtcaccttc tcggtgggca agacaatgaa ggaaaacacc
     2881 ggacaaacag cagtttattc tgccggtgag catcttgaac tctgctgtgg taaggcaagg
     2941 ctggtgctga cgaaggacgg aagcatattt ctcaatggta cgcacattca tctggaaggg
     3001 gagtcggatg tgaacggtga tgcgccagtg attaactgga actgtggtgc cacacaacct
     3061 gtaccggatg cgcctgtgcc gaaagattta ccccccggaa tgccggatat gcggcaattt
     3121 tgagtcaggt gcaaaaaatt aaaacggaac tggtgcagtg accagataaa ccatattaaa
     3181 agcagaagga attgattcat gggcggaaaa ccggcggcgc ggcagggtga tatgacccgc
     3241 aagggactgg atattgtgca gggttcagcg ggggtgctga taggtgcgcc gacgggagtg
     3301 gcctgttcgg tatgtcccaa cagcccgata gaggagcaga agggtaaccc ggtgaacccg
     3361 ctgctgggag caaaggtgct gccgggcgag acggaccttg cgctgcccgg cccgctgccg
     3421 tttattcttt cccgcgccta cagcagctac cggaccagaa cacccgcgcc ggtgggggtg
     3481 tttggtcccg gctggaaagc gcccttcgat atccgcttac agatacgcga tgaaggcctg
     3541 atactcaacg acagcggcgg caggagcatt cactttgagc cgctgtttcc cggcgagata
     3601 agctacagcc gcagcgagtc actctggctg gcgcggggcg gagtagcggc gcagcacagt
     3661 tcgcagccgc taagcgcgct ctggcaggtg ctgccggaag atgttcgcct gagtccgcat
     3721 gtgtacctgg cgacaaacag ccttcagggg ccgtggtgga tactcagctg gccggagcgg
     3781 gtgccggggg cggacgaggt gctgccgccg gagccgcccg cataccgggt gctgacgggc
     3841 gtggtggacg gcttcgggcg gacgctggcc tttcaccggg cggcggaggg tgatgtggcg
     3901 ggcgcggtga cgggggtgac ggacggcgcg ggccgccgtt ttcacctggt gctgaccaca
     3961 caggcacagc gggcggaagt attccgtaaa cagcgcgcca cgtctttatc ttcccctgcc
     4021 ggtccccgtt ctgcttcctc ttctctggtt ttccccgaca cgctgcccgc cggtacagga
     4081 tacggcacgg ataacggtat ccggctggag gcggtatggc tgacacacga cccggcatac
     4141 ccggatgaac agccgactgc gccgctggcg cgctacacgt acacggccgg tggtgaactg
     4201 cgggcggtgt atgaccgcag cggaacgcag gtgcgcgggt ttacttatga tgcggagcac
     4261 gccgggcgga tggtggcgca ccattatgcg gggaggccgg agagctgcta ccggtatgat
     4321 gataccggtc gggtgacgga gcaggtcaac ccggaggggc tggactaccg ctttgagtac
     4381 ggagagagcc gggtaatcat cacggacagc ctgaaccggc gggaggtgct gtacacggaa
     4441 ggcgagggtg gcctgaagcg ggtggtgaag aaggaacatg cggacgggag cattacccgc
     4501 agcgagtatg atgaggcggg gaggctgaag gcacagacgg atgcggcggg gcggcggacg
     4561 gagtacagcc tgcatatggc gtcgggtgcg gtgacggcgg tgacggggcc ggacggcagg
     4621 acggtgcggt atggctataa cagccagcgg caggtgacgt cagtgacgta cccggacggg
     4681 cttcgcagca gccgggagta tgatgagagg ggaaggctga cggcggagac ctcgcgcagc
     4741 ggtgagacaa cgcggtacag ctatgatgac ccggcgagtg agctgccgac agggatacag
     4801 gacgcgacgg gcagtacaaa acagatggca tggagccgtt acggtcaact gctggccttc
     4861 acggactgct cggggtacac gacgcggtat gagtacgacc ggtacggtca acaaatcgcc
     4921 gttcaccggg aggaaggcat cagcacttac agcagttata acccgcgtgg gcaactggtc
     4981 agtcagaagg atgcgcaggg gcgtgaaatc cgttatgagt acagcgccgc aggcgacctg
     5041 acagcaacgg tatcaccgga cggtaaacgc agcaccatcg aatatgataa acgcgggcgt
     5101 ccggtgagtg tgacggaagg cggtctgacg cgcagcatgg ggtatgacgc tgccgggcgc
     5161 atcactgtgc tgaccaacga gaacggcagc cagtccacgt tccggtatga cccggtcgac
     5221 aggctgactg aacagcgcgg ttttgacggc cggacgcaac gttaccacta tgacctgacc
     5281 agaaaactca c
//
LOCUS       DAZFXT010000215         5290 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.215, whole genome shotgun sequence.
ACCESSION   DAZFXT010000215 DAZFXT010000000
VERSION     DAZFXT010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.215"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            121..1479
                     /gene="mltD"
                     /locus_tag="ACHKW0_004757"
     CDS             121..1479
                     /gene="mltD"
                     /locus_tag="ACHKW0_004757"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008933 - lytic transglycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HFO7477428.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSSVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(1527..2297)
                     /locus_tag="ACHKW0_004758"
     CDS             complement(1527..2297)
                     /locus_tag="ACHKW0_004758"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7477429.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVCLADYP
                     DASVLDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTLEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2375..3175)
                     /locus_tag="ACHKW0_004759"
     CDS             complement(2375..3175)
                     /locus_tag="ACHKW0_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HFO7477430.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            3416..4330
                     /gene="yafC"
                     /locus_tag="ACHKW0_004760"
     CDS             3416..4330
                     /gene="yafC"
                     /locus_tag="ACHKW0_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YafC"
                     /protein_id="HFO7477431.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMASAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(4327..5130)
                     /gene="dkgB"
                     /locus_tag="ACHKW0_004761"
     CDS             complement(4327..5130)
                     /gene="dkgB"
                     /locus_tag="ACHKW0_004761"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HFO7477432.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT         1390 a         1275 c         1312 g         1313 t
ORIGIN      
        1 acctgaagaa cgttttgcat ggttaaggtc aaagaaagat aggttctgat aaaactttct
       61 tgtcatcggc tccgttcgcc gttatgatcg gtcgtctttt aagcaactat tgacacacac
      121 atgaaggcaa aagcgatatt actcgcctct gtcctgctcg tgggttgcca gagtaccggc
      181 aacgttcaac agcacgcaca gagcctttct gcagctggtc aaggggaagc agcaaagttt
      241 acaagtcagg cacgatggat ggacgatggg acgtctatcg cgccagatgg tgacttgtgg
      301 gctttcattg gcgacgagct aaagatggga attccggaaa atgaccggat tcgcgaacag
      361 aaacagaaat atttacgcaa taagagctat ctccacgatg taactttacg ggcagagccg
      421 tatatgtact ggatagccgg gcaagttaaa aaacgtaaca tgcctatgga actggtacta
      481 ctacccatag tggagagcgc ttttgatcct cacgcaacgt ctggcgccaa tgccgcaggc
      541 atctggcaga tcattccgag cacggggcgc aattatggtt tgaaacagac ccgcaattat
      601 gacgcgcgtc gcgatgttgt tgcttcaaca actgccgcgc tgaacatgat gcagcgtctg
      661 aacaagatgt ttgacggcga ctggcttctg accgtagcgg cttataacag cggcgaaggt
      721 cgggtcatga aggcaattaa aacgaacaaa gcgcgtggga aatccacgga cttctggtcg
      781 ttaccgttgc cgcaggaaac gaagcagtac gtgcctaaaa tgctggcatt gagtgatatt
      841 ctcaaaaaca gcaagcgtta tggcgtacgt ctgccaacga ccgatgaaag ccgtgctctg
      901 gcgcgtgtgc acctgagcag cccggttgaa atggcgaagg ttgcagatat ggcggggatt
      961 tccgtcagca agctgaagac attcaacgct ggcgtgaaag gctccacgct gggcgcaagt
     1021 ggtccgcaat acgtgatggt gccaaagaag catgcagatc aactgcgtga atctctcgct
     1081 tcaggcgaaa ttgctgctgt acagtcgacg ctggttgccg acaatacgcc gcttaacagc
     1141 agtgtttaca ccgtacgctc tggcgacacg ctttcaagta tcgcttcacg tctcggcgta
     1201 agcaccaaag atttgcagca gtggaacaaa ctgcgcggat ctaagctgaa gccaggccaa
     1261 agtttgacga ttggtgcagg cagtagcgca cagcgactgg caaacaacag cgatagcatt
     1321 acgtatcgtg tgcgcaaagg cgattcgctt tcaagcattg ctaaacgcca cggcgtgaac
     1381 atcaaagatg tgatgcgctg gaacagcgac actgcgaatc tgcaaccagg cgacaagctg
     1441 acgttgtttg tgaaaaacaa caacatgcca gattcctgac aaaccagata ataaaaaggc
     1501 accgattccc ccggtgcctt ttttatttat gctgctttat gtgcttctac catgatgata
     1561 tcactggtga aagagccatc attctgcaag gcaaaatacg ttctcacctc tgtcgatgcg
     1621 ctctgttggt aaatgcgaat agcgtctact aacgcttcta gcgtacgcat tctcgcgacc
     1681 catgaagaaa attccagcgg taacttatct gtaattaaat tatcaactat cagattggct
     1741 tcattgatta acctcaacca ctcaccgctg gcgtagtttc gtacgtgaga ggtatcgcgt
     1801 aatgcttcta ccgtctgtaa ccagatgtcg cgcactggat gacccggaga cattacgtcc
     1861 atcacaatca tcctaccgcc aggtttcaat atcctattca cttctcgcag tgctgcacca
     1921 acatcatgcc agtgatgggc agaataacgg ctgataacaa tatcaaatgc gttatcggca
     1981 aatggcagac tttcggcata accctggcgg gtggtgatat ttttcagttg ccgggcttcg
     2041 gcagcttgtg ccacgacatc cagcatttgg gcagataagt catacgccac caccgcgctc
     2101 acgttctgcg cagcgacaaa gctggcatgc cctgctccgc agcccatatc aagtacactt
     2161 gcatcaggat aatcagccag acacaccgcc aggcgctgta aatctcgccc ggatgcatgt
     2221 acggtactgg ttaaatactc actggcctgc gagctaaact gcttttctac gtggtcatgg
     2281 tgggattgtg ttgtcattgc gctgtccttt tgttgtttga gtataaaagg acagcgccaa
     2341 acgttggtct gccctgtggc agacctgaca taccttattt atcaggcttg ccgggactga
     2401 attcaacgag tagcggattg tgatcggaag cgcgcgtaac caatacagaa gcttcgctga
     2461 cgttcagacc acggtagaaa acaaaatcga gcgggcggcc aaacgcccga cggcgctgat
     2521 catcggtaaa acgcacctgg cgcagcgaca tttcccgcgc aaagcgatat aacgcgttca
     2581 tccttctacg gctccaggca ttgaaatctc ctgccataat gaccgggccg ctgtggtgag
     2641 ctatctgatc accaatagga agtaactgct tactatagac atccacgccc agactgaagt
     2701 tgacggcgtg tatattaacc accatcaaca ggcgggtgtc aggtaatgga tagaccgtca
     2761 ccagtgccga cttcgccaga cgcaaaatgg gttctcgttc acgtaacggg cagcaataca
     2821 ctggatgcgc cgccgaaagg gtcattacgc cagaaggatg ttgtggcagt acgaaagcgg
     2881 gtacctgatc ggcggcaaga tagttagcgg tcgcaaactg tactaactct ggcgttgtct
     2941 gcgcttcctg caataacacc agatgtgcat ctttgccgta gttctttaat accgacaacc
     3001 attcagcgcg ttgctgtttg tatatgttcc acaccaggat ccgaatacgc tcttcggtac
     3061 ttaacggttc tccgggtggt aatgcctggc cgatgctcgc aaaagacccc ggcggtaaga
     3121 tcctttccgc aggttgtccg gcaacatagc gcatggcata ggtgtttttt cgcacttttg
     3181 actttgaaac ctctattacg tcaacccttc cgcactcggg agggttctac tgttataggg
     3241 attttagctc actctttcaa cgagcaattg tatattgtta tgtaagcaag tgcttacacc
     3301 ggtcagaatt ttgctcatta tggagaacat taaatcgcat gacatatcac gcatcctgat
     3361 ggtttaatga caaataaatt caaaatatct cttctatttt gcaacaggag caaaaatgaa
     3421 agccacgtcg gaagaactcg ccatttttgt ttcggtcgtc gaaagcggca gctttagccg
     3481 ggcagcggaa caattagggc aagcaaactc agcggtaagc cgggcggtga aaaagctgga
     3541 gatgaaactt ggcgttagcc tgcttaatcg gaccacgcga caacttagcc tgacggaaga
     3601 aggcgagcgt tattttcgtc gcgtacagtc aattttgcag gagatggcat cggcagaatc
     3661 agaaattatg gagacgcgta atacaccgcg tggactgtta cggatcgatg ccgcaactcc
     3721 agtggtgctg cactttctga tgccgttaat taagcctttc cgtgaacgct atccggaagt
     3781 cactttgtcg ctagtctcct ccgaaacgat tattaatttg atcgaaagaa aagtggatgt
     3841 cgcgatacgc gctggtacgt taacggattc cagcttacgt gccaggccgt tatttaacag
     3901 ttatcgaaaa attatcgcct cccccgatta tatttcccgc tacgggaagc cagaaacgat
     3961 cgacgattta aagcaacata tttgcctggg attcactgaa cccgcttccc tcaatacctg
     4021 gccgatagcc cgtagcgatg gacaattaca tgaggtgaag tacggtttgt catccaatag
     4081 tggggaaaca ctgaaacagc tttgcctgag tggcaacggg attgcgtgtt tgtccgacta
     4141 catgatcgac aaagaaatcg ctcgcggaga attggtggag ttaatggcag ataaagtgtt
     4201 gccagtggaa atgccattca gtgcagtcta ttacagcgac cgtgcggtaa gtacgcgcat
     4261 ccgggctttt atcgatttcc ttagcgagca tgtaaaaaca gctcccggag gagctgtcag
     4321 agaggcttaa tcccattcag gagccagacc ttccgggcta accaggcggt cgttgcaatc
     4381 cagtgcggcg atcgcttttt tatcttcggc atcaagctgt aaattttgtg ccttaagatt
     4441 actttccagg ttttcacgtt tagtagaaga aggaattact gagtaacctt cccccatagc
     4501 ccacgccaga atcacttgtg ccggagtcgc attgtgttta gctgcgatac gagcaataac
     4561 ctcatctttc agggccttac catacgccag cgtcatatag gaagtaatat ggatgccgtg
     4621 ctgtttagcc cagacaacca ctttacggtt ttgcagataa ggagagagtt caatctggtt
     4681 agtagcgatg ttttcagcgc caacagcagc aatcgttttt tccatcaacg ggatcgtgaa
     4741 gttggaaata ccgatctcac gtgtcagacc ttgttttttg gcttccagca gcgcctgcat
     4801 aaactcttca acagagactt catcgtttgg tgacggccag tggattagcg tcagatcaac
     4861 ataatcggta cgcaattttt gcaggctctc tttcagactt gggatcaatt tgtctttgct
     4921 gagattttca atccagattt tagtggtgat gtagagttca tgacgtggca cgccactttc
     4981 tgcaatcgcc tgacctactg cggcttcgtt atcatagatt tgtgcggtat caattgcgcg
     5041 ataaccaagt tcaagcgccg ttttcacaga tgaaataaca acgtcgtctt tcagacggaa
     5101 agtacctaaa ccaaatgcag ggatagccat aatatgcctc ttttaatgct attttcgtta
     5161 actgaggtga ttatgggctt cagactgttg cggaaaaaga gcgtaaaacg cagaggattt
     5221 ttgcgattct ggcaataata gaaatgtaga cgaaaaaaaa cctcgagcga taactcgagg
     5281 cttcttaata
//
LOCUS       DAZFXT010000216         5235 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.216, whole genome shotgun sequence.
ACCESSION   DAZFXT010000216 DAZFXT010000000
VERSION     DAZFXT010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.216"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..402
                     /locus_tag="ACHKW0_004762"
     CDS             <1..402
                     /locus_tag="ACHKW0_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO7477433.1"
                     /translation="IIKECIENGINSTPSTGYQPLILQSIKTKLLSSRLNKFNDFEDS
                     FNGLGISVHDISAQKISLLSFQNYAIGWSATIHFVAQDHFGLDVTDIKNKTYSKYRFF
                     RIWFFLQRHKDFAFKPFFTNFNTIERIENYL"
     gene            399..890
                     /locus_tag="ACHKW0_004763"
     CDS             399..890
                     /locus_tag="ACHKW0_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HFO7477434.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKK
                     DNF"
     gene            complement(932..1882)
                     /gene="corA"
                     /locus_tag="ACHKW0_004764"
     CDS             complement(932..1882)
                     /gene="corA"
                     /locus_tag="ACHKW0_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HFO7477435.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(2034..2090)
                     /gene="ysgD"
                     /locus_tag="ACHKW0_004765"
     CDS             complement(2034..2090)
                     /gene="ysgD"
                     /locus_tag="ACHKW0_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_010051213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YsgD"
                     /protein_id="HFO7477436.1"
                     /translation="MDTPSRYWLTILSSRINS"
     gene            2252..3016
                     /gene="yigE"
                     /locus_tag="ACHKW0_004766"
     CDS             2252..3016
                     /gene="yigE"
                     /locus_tag="ACHKW0_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HFO7477437.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            complement(3163..>5235)
                     /gene="uvrD"
                     /locus_tag="ACHKW0_004767"
     CDS             complement(3163..>5235)
                     /gene="uvrD"
                     /locus_tag="ACHKW0_004767"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HFO7477438.1"
                     /translation="AGSGKTRVLVHRIAWLMSVENCSPYSIMAVTFTNKAAAEMRHRI
                     GQLMGTSQGGMWVGTFHGLAHRLLRAHHMDANLPQDFQILDSEDQLRLLKRLIKAMNL
                     DEKQWPPRQAMWYINSQKDEGLRPHHIQSYGNPVEQTWQKVYQAYQEACDRAGLVDFA
                     ELLLRAHELWLNKPHILQHYRERFTNILVDEFQDTNNIQYAWIRLLAGDTGKVMIVGD
                     DDQSIYGWRGAQVENIQRFLNDFPGAETIRLEQNYRSTSNILSAANALIENNNGRLGK
                     KLWTDGADGEPISLYCAFNELDEARFVVNRIKTWQDNGGALAECAILYRSNAQSRVLE
                     EALLQASMPYRIYGGMRFFERQEIKDALSYLRLIANRNDDAAFERVVNTPTRGIGDRT
                     LDVVRQTSRDRQLTLWQACRELLQEKALAGRAASALQRFMELIDALAQETADMPLHVQ
                     TDRVIKDSGLRTMYEQEKGEKGQTRIENLEELVTATRQFSYNEEDEDLMPLQAFLSHA
                     ALEAGEGQADTWQDAVQLMTLHSAKGLEFPQVFIVGMEEGMFPSQMSLDEGGRLEEER
                     RLAYVGVTRAMQKLTLTYAETRRLYGKEVYHRPSRFIGELPEECVEEVRLRATVSRPV
                     SHQRMGTPMVENDSGYKLGQRVRHAKFGEGTIVNMEGSGEHSRLQVAFQGQGIKWLVA
                     AYARLESV"
BASE COUNT         1304 a         1360 c         1258 g         1313 t
ORIGIN      
        1 ataatcaaag aatgtatcga aaatggcatt aattcaactc cctcgacagg ataccaacct
       61 cttatcctgc aaagcattaa aaccaaatta ctttcatcaa gactaaataa atttaatgac
      121 ttcgaggaca gttttaatgg cttaggaatc agtgttcatg atatttcagc tcaaaaaata
      181 tctcttttaa gttttcaaaa ctacgctata ggatggagtg caacaattca ttttgtagca
      241 caggaccact tcggtcttga tgtaactgat atcaaaaaca agacttacag caaatataga
      301 ttttttcgca tatggttctt tttacaacgg cataaagact ttgctttcaa accttttttc
      361 accaatttta acactatcga aagaatcgaa aattatctat gaaaagcaaa ctccagaaaa
      421 ttatattatg tttatttctt ctatgctgca tctataattt atggacattg cgtcctgtac
      481 agattttgta tacatactct gatgctggca attcagtctt tctggtcgtt gatcatctgc
      541 catggactga cagcgataaa ataaattggt atctgaagca tcagaatgaa attaaaaatc
      601 aacatcccct accagaaggt agttggcata cctggtatgt cattgatatc ggtaatggct
      661 tcactgacta taaaaaatat atcgaagggc cgtatgaaga tctctactgt ttcccgacaa
      721 ttaagagcaa tgataattgc atcgtgaaaa attacttaat ggttattaat gaataccctt
      781 atcgtaacac tcatattggt attaacgatt ttactgagta tcaattaaca caagaaaata
      841 aaatagagcg agttttcaat ccgcatgact ttaaaaaaga caatttttag aaataagccg
      901 gataaaaaaa tagctttatc cggcactcat attacaacca gttcttccgc ttaaagtaca
      961 gatacggtgc caggcccgcg aggatcataa agataatcgc gcctgggtag ccgaaactcc
     1021 acttcaactc tggcataaat tcaaagttca tcccgtagct ggaagccacc agcgtcggtg
     1081 gcaggaacac cacggatacc accgagaaga ttttgatgat gcggttctgc tcgatgttga
     1141 taaagcccat cgccgcctgc atcaggaagt tcaccttctg gaacagggat tcgttatgcg
     1201 gcagcaggga ttcgatatct cgcaggattt cacgcgcctg ctccagttgc ccacccggta
     1261 aacgcgcctt gcgtaccagg aagttgagcg cgcgctgggt atccatcaga cacaggcgaa
     1321 ctttccagcc gatatcttcc agttccgcca gcgtggagag cgcctcgtcg tactcatcgc
     1381 cctgatgccc ttccataatc acccggctca actgctccag gtcgctatag atattttcaa
     1441 tttcatctgc caactgttcg attttggttt cgaacagatc cagcagcaac tcgtaggcgt
     1501 taccgtctac catcgactgg ctacgggcac gcatacgata cagacgaaaa gcgggcagtt
     1561 cacgctcacg cagagtaaac agacgaccat cacggatggt aaatgccaca gtggagttac
     1621 cggcgtgatc ttccgcatct tcaaagaaga agaaggagtg aatatgcagg ccgtcgtcgt
     1681 cttcaaagaa acgtgccgat gcttcgatgt cttccagttc cgggcgggtt gccaggctct
     1741 ggccaagttc agattgtacg cgcagtcgct cgtcgtcgtc cggttcgaca agatcaatcc
     1801 atactgcatt tacaaggggt tgtgactctt cgacttccag ccgggtcagt cggttatttt
     1861 ccagttgaaa tgcgctcagc atgaccggga ctcccaatgc ttaaaatatc ggacagttcg
     1921 gtgggcacac agaaacaaat tgggtttcag accattaaac agcctgactc agcgcgacgg
     1981 gaaaaattga ggtcgctgac aaccgctaag gctatcagca aaaagggata gccttaggag
     2041 ttgatcctgg atgacaggat agtgagccag tatctactgg gtgtgtccaa ggcgaatgtc
     2101 ctcttagagt gatcgtgcgc gcatgttacg ccagcaaaat tttgccgtca acacgcaaca
     2161 gaacaccaca gaacaatagt tgaacttcgc gctttttggt actcgtcaag gtcgctaaca
     2221 ggataaaaaa atccaacatc caggacctat aatggcgcat cggctactca ttggtaaagg
     2281 aatgatcacc ttgaatctca aacggatttt tctcaccctc accttacttc ccttgtttgc
     2341 tgttgccgct gatgattgcg cactctcaga tccgacgctg accgtacagg cgtataccgt
     2401 taatcctcaa acagagcggg tgaaaatgta ctggcaaaaa gctaatggcg aagcgtgggg
     2461 aacgttacat gctctgctgg cggatatgaa tagtcagggt caggtgcaga tggcgatgaa
     2521 cggcggcatc tatgatgaaa gctatgcgcc gctcggtttg tacatcgaaa acggtcagca
     2581 gaaggtggcg ttaaatctcg cttcaggtga agggaatttc tttatccgtc ctggcggcgt
     2641 gttttatgtc gcgggagata aagtcggcat cgttcgtctg gatgccttca aaaccagtaa
     2701 agagattcag tttgcggtgc agtcagggcc aatgttgatg gaaaacggtg taattaatcc
     2761 gcgtattcat cccaacgtcg cctcacgcaa aattcgtaac ggtgttggga ttaataaaca
     2821 tgggaatgcc gtgtttttgt tgagccagca ggcgacaaat ttttatgatt ttgcctgtta
     2881 tgccaaagcg aaactgaacg ttgagcagct gctttatctt gatggcacga tttcacatat
     2941 gtatatgaaa ggcggggcaa tcccgtggca acgctatcct tttgtcacca tgatttccgt
     3001 cgagcggaaa ggttagaatg atagttttgt gtcagatact aaatatgctg ctgatgccgg
     3061 atgcggcgta aacgccttat ccggcctaca tgacgttgca atttattgaa tctgaatgat
     3121 tttttaggcc aaataaggtg cgcagcaccg catccggcaa cgttacaccg actccagccg
     3181 ggcataagcc gccaccagcc atttaatacc ctggccctga aatgccacct gcaaacggct
     3241 atgctcaccg ctgccttcca tattgacaat ggtgccttca ccaaacttag cgtggcgtac
     3301 gcgctggccg agcttgtagc cgctgtcgtt ctcgaccatc ggcgtaccca tccgctgatg
     3361 gctgaccggg cggcttaccg tggcgcgcag gcgcacctct tccacacact cttccggcag
     3421 ctcgccaata aagcgcgacg ggcgatggta aacctctttg ccatacagac ggcgagtttc
     3481 cgcgtaggtc agcgtcagtt tctgcatcgc gcgggttacg ccaacgtagg ccagacggcg
     3541 ttcttcttcc agacgcccgc cttcatccag cgacatctgg cttgggaaca taccctcttc
     3601 cataccgaca ataaacacct gcgggaactc caggcctttc gccgagtgca gcgtcatcaa
     3661 ctgcactgca tcctgccagg tatccgcctg cccctcgccc gcttccagcg ccgcatggga
     3721 gaggaatgcc tgcagcggca ttaaatcttc atcttcttcg ttgtagctga actggcgcgt
     3781 tgccgtcacc agttcctcta agttttcgat acgcgtctgg cctttttcgc ccttctcctg
     3841 ctcgtacatg gtgcgcaggc cggagtcttt aattacccgg tcagtctgta catgcagcgg
     3901 catatcggca gtttcttgcg ctaaggcatc gattaattcc ataaatcgct gcaaggcgct
     3961 ggcagctcgc ccggcgaggg ctttttcctg caacagttca cgacatgcct gccagagtgt
     4021 taactggcga tcgcgcgatg tctgacgtac cacgtccagc gtccggtcac caataccccg
     4081 cgttggcgta ttcaccacgc gctcaaacgc cgcgtcgtcg ttacggttgg caatcagacg
     4141 cagatacgag agcgcgtctt tgatttcctg gcgttcgaag aagcgcatcc cgccgtaaat
     4201 acggtacggc atactggcct gcaacagtgc ttcttccagt acacgcgact gggcgttact
     4261 gcggtagaga atggcgcact cggcaagcgc cccgccgttg tcctgccagg ttttgatgcg
     4321 gttaaccaca aaacgcgctt catcgagttc gttaaaagcg caatagaggg aaataggctc
     4381 accgtccgcg ccatcggtcc acagtttttt acccagacgc ccgttattgt tttcaatcag
     4441 ggcgttagcg gcgctcagaa tattgctggt agagcggtag ttttgctcca gacgaatagt
     4501 ttcggcaccg gggaaatcat taaggaaacg ctgaatattc tccacctgcg ccccgcgcca
     4561 gccgtagatt gactggtcgt catcaccgac gatcatcact ttgccggtgt cgcccgccag
     4621 caggcggatc cacgcgtact gaatgttgtt ggtatcctgg aattcgtcca ccaggatatt
     4681 ggtaaaacgt tcgcggtagt gttgtaggat atgcggcttg ttaagccaca gttcgtgagc
     4741 gcgcagcagc agctcggcga agtccaccag gcccgcgcgg tcacacgctt cctgatacgc
     4801 ctgatacacc ttctgccagg tctgctccac cggattaccg tagctttgaa tatgatgcgg
     4861 acgcaggcct tcatctttct ggctgttgat gtaccacatt gcctggcgcg gcggccactg
     4921 cttctcgtcg aggttcatgg ctttgatcag acgcttaagc aggcgcagct ggtcctcgct
     4981 atcgaggatc tggaaatcct gcggcagatt ggcgtccata tggtgcgcac gcagcaggcg
     5041 gtgcgccagc ccgtggaagg tgccgaccca catgccgccc tggctggtgc ccatcagttg
     5101 cccgatacga tgacgcatct ccgccgccgc tttgttggta aacgtcaccg ccataatcga
     5161 gtatggcgag cagttttcca cgctcatcag ccaggcgata cgatgcacca gtacgcgcgt
     5221 cttaccactg cccgc
//
LOCUS       DAZFXT010000217         5187 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.217, whole genome shotgun sequence.
ACCESSION   DAZFXT010000217 DAZFXT010000000
VERSION     DAZFXT010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5187
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.217"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            305..469
                     /locus_tag="ACHKW0_004768"
     CDS             305..469
                     /locus_tag="ACHKW0_004768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477439.1"
                     /translation="MNQLPRRALSRYGLTFQGNILSVNCQCRMLTRVRRKHSTSPVEN
                     SLITNLSFVG"
     gene            complement(473..691)
                     /gene="ydfC"
                     /locus_tag="ACHKW0_004769"
     CDS             complement(473..691)
                     /gene="ydfC"
                     /locus_tag="ACHKW0_004769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO7477440.1"
                     /translation="MQKREPVIIAPDYTNDELYEWMHQKINAAQDLKWANEVRAKQAE
                     NLSSLEQDITNLEKAAALTIARMITYPR"
     gene            complement(721..849)
                     /locus_tag="ACHKW0_004770"
     CDS             complement(721..849)
                     /locus_tag="ACHKW0_004770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477441.1"
                     /translation="MDFDTIMEKAYEEYFEGLAEGEEAHSFNEFKQVLSSSAKSNG"
     gene            complement(851..1006)
                     /locus_tag="ACHKW0_004771"
     CDS             complement(851..1006)
                     /locus_tag="ACHKW0_004771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7477442.1"
                     /translation="MDTIDLGNSESLVCGVFPNQYGTFTAMTYTKSKTFKTESGARRW
                     LERNSGG"
     gene            complement(1325..1801)
                     /gene="racR"
                     /locus_tag="ACHKW0_004772"
                     /gene_synonym="rarC"
     CDS             complement(1325..1801)
                     /gene="racR"
                     /locus_tag="ACHKW0_004772"
                     /gene_synonym="rarC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor RacR"
                     /protein_id="HFO7477443.1"
                     /translation="MLSGKDLGRAIEQAINKKIASGAVKSKAEIARHFKVQPPSIHDW
                     IKKGSISKDKLPELWRFFSDVVGPEHWGLNEYPIPTPSTSDTKSELLDINSLYQAASD
                     EKRAIVAFILSGNATEPSWVDHDVRAYIAAMEMKVANYLKNQESKRKSQNITKTGT"
     gene            1926..2249
                     /locus_tag="ACHKW0_004773"
     CDS             1926..2249
                     /locus_tag="ACHKW0_004773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7477444.1"
                     /translation="MKKDNYSFKRACAVVGGQSAMARLLGVSPPSVNQWIKGVRQLPA
                     ERCPAIERATKGGVLCEELRPDVDWTYLRRSSCYSQNMSMKQPNDENDHTRSIKRQMI
                     HENQT"
     gene            2233..2658
                     /locus_tag="ACHKW0_004774"
     CDS             2233..2658
                     /locus_tag="ACHKW0_004774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7477445.1"
                     /translation="MKIKHEHIRMAMNAWAYPDGEKVPAAEIARTYFELGMTFPELYD
                     DSHPEALARNTQKIFRWLDKDTPDAVEKMQALLPAIEKAMPPLLVARMRSHSSEYYRE
                     IVERRDRLVKDVDDFVASAVVLYDQMNRGGPAGNAVVMH"
     gene            2681..3643
                     /locus_tag="ACHKW0_004775"
     CDS             2681..3643
                     /locus_tag="ACHKW0_004775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7477446.1"
                     /translation="MSSKLHGLVWEGCAFTGMILSRVAVMARLADYSNDEGVSWPAIE
                     TIRRQIGARSESTVKSAIAELAKEGWLTKEERKVGGRNVSNIYRLNVEKLEAAAAAAR
                     ESYKPKRKISPAKNDPLTVDPSNIDPSTVDPSNFDGSTVDKKLPIRGAMIDPDPSVLK
                     PDPSDKRSSCPDASQPDPQTAEQDFLTRHPDAVVFSAKKRQWGSQEDLVCAQWIWGRI
                     VSLYEQAASDDGEITRPKEPNWTAWANDVRTMRMLDGRTHRQICEMFGRLQRDSFWVK
                     NIMSPAKLREKWDELVIRLGRSPAQRCVNHISEPDTEIPPGFRG"
     gene            3650..4390
                     /locus_tag="ACHKW0_004776"
     CDS             3650..4390
                     /locus_tag="ACHKW0_004776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000788752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7477447.1"
                     /translation="MKNIAAVGVLERIRRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRLNHQVRVEKILNRAGIQPLHRKCSFGNYRVQNDGQRHALSQAKSIADELMTGCT
                     NFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVTVSDVMSVLHDGYDNGQSGEKFLQE
                     LCGVDLLVLDEIGMQRDTRNEQVTLNQIVDRRTASMRSVGMLTNLNHAAMSTLLGDRV
                     MDRMTMNGGRWVNFNWESWRSNVGRQGM"
     gene            4416..5186
                     /locus_tag="ACHKW0_004777"
     CDS             4416..5186
                     /locus_tag="ACHKW0_004777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7477448.1"
                     /translation="METVLHALKAMGKANSVELAARLDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGEAGVTEGQALQPEAPDVVTEEVAPKVTADMMVEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMSGGNLPADPKAAPVTKNDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLLSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
BASE COUNT         1306 a         1174 c         1413 g         1294 t
ORIGIN      
        1 ccataacact gtttttggtt ttaactgttc cgcgtgcgct tagccgcatt caccgcatca
       61 caaaattcac tttaaaaagg gcggcagagc agtcacggag taaaactgat accgccaaac
      121 gtcaccagaa aattgataac agagggcgtt gcagcggggt tgtcacttaa gcgtatggtc
      181 aacctgacaa cccggtgtcc tcaacggggg aaggaataac cccgccatac ttaccgccgc
      241 gccatttcgc ggattgccac aaccggaagc gcacggtcga cgaaaattta acgacaggct
      301 atctatgaac cagctacctc gccgtgcgct ttcgcgttat ggtctgactt ttcagggaaa
      361 tatcctttca gtaaactgtc agtgccggat gctcacccgt gtccggcgca agcactccac
      421 ctcacccgtg gagaactcct taattaccaa ccttagcttc gttggttagc tattaacgcg
      481 ggtatgtaat cattctggca atggttaatg ccgctgcttt ttccagattg gtgatatcct
      541 gctccagaga ggacagattt tcagcctgct tagccctgac ttcattagcc catttcagat
      601 cctgcgctgc attaattttc tggtgcatcc actcataaag ttcatcattg gtatagtctg
      661 gcgcgatgat gacgggttct cgtttctgca tactgattcc tcgcggtgct gtttcgctta
      721 tcagccatta gattttgccg aactggaaag cacctgttta aattcgttga agctgtgagc
      781 ttcttcgcct tcggcaaggc cttcgaagta ttcttcgtaa gccttttcca tgattgtgtc
      841 gaaatccata tcacccacct gaatttcttt ccagccagcg acgcgctcca gattcggttt
      901 taaacgtttt gcttttggta tacgtcattg cggtgaacgt accgtactgg ttggggaaca
      961 cgccacatac cagagattcg ctgttgccaa gatcgatagt atccatgctg acctcatttc
     1021 cccttaacgc cggggtagcg gaacaaaaac ctgctgcata gttattaaag ttgaaccctg
     1081 ccgtcatgtt cttacgcctc gggctggcta cttaacccgt gaccactgcc tggtaactcg
     1141 aggtattgcc ctgtattgtg tgggacggga tgggttggta tgggaaaact ataggaaatg
     1201 cctaattact tgtcaatagg ctatgcctaa taatttgggc gcaacctaat aggtgatggt
     1261 ttgtggaaga ggtagtagga gttaactaac gggaactagg aatttcccgt cggaccatat
     1321 aagtttaagt tcctgtcttg gtgatgttct ggcttttccg ttttgattct tgatttttca
     1381 gatagttagc taccttcatt tccattgcgg caatgtaggc gcgaacgtca tgatcaaccc
     1441 aactaggctc cgtagcattt ccagataaga tgaaagccac aattgctctt ttttcatcag
     1501 aggcggcttg ataaaggctg tttatgtcta aaagttcact ttttgtatct gaagtggatg
     1561 gggttggtat ggggtattcg ttaagccccc aatgctctgg accaaccaca tcagaaaaga
     1621 aacgccatag ttctggaagt ttgtctttac ttatcgaacc tttcttaatc cagtcatgga
     1681 ttgatggtgg ttggactttg aaatgacgtg cgatttccgc ctttgatttg acggctcctg
     1741 atgcaatttt tttgttaatg gcctgctcta tcgctcggcc taagtcttta ccactaagca
     1801 ttgcttaata gtctcctatg cgcatcgcgt taggcaatcc ctactctcga tgtattaggc
     1861 atagcctatt gacaatttca ttaggctaag cctaatattg ttgcgtgttt tttggagttc
     1921 attcgatgaa aaaagataac tattcattca aacgagcttg tgctgttgtc ggtgggcaat
     1981 cagcaatggc taggctttta ggtgtatctc ctccaagcgt aaatcaatgg atcaaaggtg
     2041 tacgtcagtt acctgctgag agatgtcctg cgattgaacg agcaacaaaa ggtggtgttc
     2101 tgtgtgaaga acttcgtcct gatgttgatt ggacatactt acgacgctcg tcatgttatt
     2161 cgcagaatat gtcgatgaag caaccaaatg acgaaaacga tcatacccga agcatcaaga
     2221 ggcaaatgat tcatgaaaat caaacatgag cacatccgca tggcgatgaa tgcctgggca
     2281 taccctgatg gtgagaaagt tcctgcagct gaaatagccc ggacttattt cgaactgggg
     2341 atgacgttcc ctgaactgta cgacgacagc catccggaag ccctggcccg taatacccag
     2401 aaaattttcc gttggctgga taaagacacc cctgatgctg ttgaaaaaat gcaggctctg
     2461 ttaccggcga tcgaaaaggc gatgccgcct ttgctggtgg cccgtatgcg cagccacagt
     2521 tctgaatatt accgtgagat cgtcgaacgg agggatcggc tggtgaagga tgtcgatgat
     2581 tttgttgcgt cagcggttgt tttgtatgac cagatgaatc gcggcggccc ggcagggaat
     2641 gctgtggtga tgcactaaaa gcacggtgtt cgggggtttt atgagcagca agcttcatgg
     2701 tcttgtctgg gaagggtgcg ccttcaccgg catgatctta tccagggtgg cggttatggc
     2761 ccgtcttgca gactacagca atgacgaggg cgtgtcatgg cctgccattg aaactatccg
     2821 gcgtcagatc ggtgcaagaa gtgaatccac agtgaaatcg gctattgcag aactggcgaa
     2881 agagggctgg ctgacgaagg aagagcgtaa ggtcggtggg cgtaatgtaa gcaatatcta
     2941 tcggcttaat gtggaaaaac tcgaagcagc tgcggcggcg gcgcgtgagt catataaacc
     3001 gaaaagaaaa attagcccgg caaaaaatga cccgttaaca gttgacccgt caaatattga
     3061 cccctcaacg gttgacccgt caaattttga tggatcaact gttgataaaa aactgccgat
     3121 taggggggcg atgattgacc ccgatccgtc agtattaaaa cctgatccgt cagataaaag
     3181 atcttcttgt ccggacgctt cgcaaccgga cccgcagacg gctgaacagg attttttaac
     3241 ccgacaccct gacgcggttg tgttcagtgc gaaaaaacgc cagtggggaa gtcaggaaga
     3301 tttggtgtgc gcacagtgga tctggggacg aatcgtgagt ctttacgagc aggcggccag
     3361 cgatgatggc gagatcacta gaccgaaaga acccaactgg acagcatggg ccaatgacgt
     3421 tcgcacaatg cggatgctgg atggcagaac tcacagacaa atttgtgaaa tgtttgggcg
     3481 tctccagcgg gattcgttct gggtaaaaaa catcatgagt ccggcaaaac tccgggaaaa
     3541 atgggatgaa ctggttatcc gcctggggcg ttcgcctgcg cagcgttgcg tgaatcacat
     3601 ttctgaaccg gacactgaaa taccgccggg attcaggggg tgacgtgtca tgaaaaacat
     3661 tgcggcagtt ggggttcttg aacgtattcg cagacttgca ccacaggggg cggttccacc
     3721 gtaccggacg gtggaggagt ggcgggaatg gcaacttgct gaaggacgaa aacgcagcga
     3781 ggagattaac cgcctgaatc atcaggtgcg ggttgaaaaa atcctgaacc gtgcgggcat
     3841 ccagccgctt cacaggaagt gctcattcgg gaactaccgg gtgcagaacg acggtcagcg
     3901 ccatgctctg agccaggcga aatccattgc cgatgaattg atgaccggat gtacaaactt
     3961 cgtgttcagc ggtaaacctg gtaccggtaa aaatcacctg gcagcagcga ttggcaatcg
     4021 gctgatggcg aaggggagaa gcgtgattat cgtcaccgtg tccgatgtca tgagcgtgtt
     4081 gcatgacggc tacgacaacg gccagtccgg ggaaaaattt ttacaggagc tttgtggagt
     4141 tgaccttctg gtccttgatg aaattggcat gcagcgggat acgcgcaacg agcaggtcac
     4201 gctgaaccag atagtcgacc gcagaacggc ttcgatgcgt agtgtcggaa tgctgacgaa
     4261 cctgaatcac gcagcgatga gcacactcct cggagatcgg gtgatggacc gtatgaccat
     4321 gaatggtggt cgttgggtga attttaactg ggagagctgg cggtcaaacg ttggacgtca
     4381 gggtatgtga gaatttttga cgaggtaaat tttcgatgga aactgtattg catgcactga
     4441 aagcgatggg aaaagccaat tctgttgaac tggcggcgcg gcttgatatc agccgtgaag
     4501 aagttcttaa cgaactgtgg gaactcaaaa aaaatggcgt tgttgataaa acgggtcaca
     4561 cctggtttct ggctgtcgaa ggtgaagccg gggtaaccga agggcaggca ctacaacctg
     4621 aagcgccgga tgtggtaacc gaagaggtcg ctccaaaagt taccgcagac atgatggttg
     4681 agtttatcgg tcaggatggt gctaaaacgt gtgaggaact ggcgggtaag ttcggcgtca
     4741 gtactcgcaa ggttgcttcc acgctggcgg tggtaaccgc aacggggcgg ctggcacgcg
     4801 ttaatcagaa cggtaaattt cgttactgca tgtcgggggg taatttacca gcagatccga
     4861 aagccgcgcc ggtaacgaaa aatgatggta aggcctttcc tcagccagca ggtgctgcgt
     4921 taccagtccg ggaagccgca acacaggaag aaattaaaac agaaactgtg gcggacattg
     4981 tgcagccgtt gctatcgttt accgaaacgc aagcagatga gctgattttt ccgtccctgc
     5041 gcagggcaaa cctggcgctg cgcagggcga aaagtgatgt tcagaagtgg gagcgagtct
     5101 gcgccgcgct gcgggagctg aacaagcacc gggatattgt tcgacagatt actgattctt
     5161 cccgccgtgt tgtatcggaa aagtgat
//
LOCUS       DAZFXT010000218         5186 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.218, whole genome shotgun sequence.
ACCESSION   DAZFXT010000218 DAZFXT010000000
VERSION     DAZFXT010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.218"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..1425
                     /gene="codB"
                     /locus_tag="ACHKW0_004778"
     CDS             166..1425
                     /gene="codB"
                     /locus_tag="ACHKW0_004778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015209 - cytosine transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HFO7477449.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            1415..2698
                     /gene="codA"
                     /locus_tag="ACHKW0_004779"
     CDS             1415..2698
                     /gene="codA"
                     /locus_tag="ACHKW0_004779"
                     /EC_number="3.5.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HFO7477450.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(2738..3637)
                     /gene="cynR"
                     /locus_tag="ACHKW0_004780"
     CDS             complement(2738..3637)
                     /gene="cynR"
                     /locus_tag="ACHKW0_004780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HFO7477451.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALHELGAGKRAIHDVAALTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAKHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            3747..4406
                     /gene="cynT"
                     /locus_tag="ACHKW0_004781"
     CDS             3747..4406
                     /gene="cynT"
                     /locus_tag="ACHKW0_004781"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HFO7477452.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            4437..4907
                     /gene="cynS"
                     /locus_tag="ACHKW0_004782"
     CDS             4437..4907
                     /gene="cynS"
                     /locus_tag="ACHKW0_004782"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008824 - cyanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009439 - cyanate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HFO7477453.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            4940..>5186
                     /locus_tag="ACHKW0_004783"
     CDS             4940..>5186
                     /locus_tag="ACHKW0_004783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HFO7477454.1"
                     /translation="MLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFSVAALLTALP
                     VVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMR"
BASE COUNT         1126 a         1378 c         1406 g         1276 t
ORIGIN      
        1 gcctacccac tacccactgt ttttacaccg ataatttttc ccccaccttt ttgcactcat
       61 tcatataaaa aatatatttc cccacgaaaa cgattgcttt ttatcttcag atgaatagaa
      121 tgcggcggat tttttgggtt tcaaacagca aaaaggggga atttcgtgtc gcaagataac
      181 aactttagcc aggggccagt cccgcagtcg gcgcggaaag gggtattggc attgacgttc
      241 gtcatgctgg gattaacctt cttttccgcc agtatgtgga ccggcggcac tctcggaacc
      301 ggtcttagct atcatgattt cttcctcgca gttctcatcg gtaatcttct cctcggtatt
      361 tacacttcat ttctcggtta cattggcgca aaaaccggcc tgaccactca tcttcttgct
      421 cgcttctcgt ttggtgttaa aggctcatgg ctgccttcac tgctactggg cggaactcag
      481 gttggctggt ttggcgtcgg tgtggcgatg tttgccattc cggtgggtaa ggcaaccggg
      541 ctggatatta atttgctgat tgccgtttcc ggtttactga tgaccgtcac cgtctttttt
      601 ggcatttcgg cgctgacggt tctttcggtg attgcggttc cggctatcgc ctgcctgggc
      661 ggttattccg tgtggctggc tgttaacggc atgggcggcc tggacgcatt aaaagcggtc
      721 gttcccgcac aaccgttaga tttcaatgtc gcgctggcgc tggttgtggg gtcatttatc
      781 agtgcgggta cgctcaccgc tgactttgtc cggtttggtc gcaatgccaa actggcggtg
      841 ctggtggcga tggtggcctt tttcctcggc aactcgttga tgtttatttt cggtgcagcg
      901 ggcgctgcgg cactgggcat ggcggatatc tctgatgtga tgattgctca gggcctgctg
      961 ctgcctgcga ttgtggtgct ggggctgaat atctggacca ccaacgataa cgcactctat
     1021 gcgtcgggtt taggtttcgc caacattacc ggtatgtcga gcaaaaccct ttcggtaatc
     1081 aacggtatta tcggtacggt ctgcgcatta tggctgtata acaattttgt cggctggttg
     1141 accttccttt cggcagctat tcctccagta gggggcgtga tcatcgccga ctatctgatg
     1201 aaccgtcgcc gctatgagca ctttgcgacc acgcgtatga tgagtgtcaa ttgggtggcg
     1261 attctggcgg tcgcgctggg gattgccgca ggccactggt taccgggaat tgttccggtc
     1321 aacgcggtat taggtggcgc gctgagctat ctgatcctta acccgatttt gaatcgtaaa
     1381 acgacagcag caatgacgca tgtggaggtt aacagtgtcg aataacgctt tacaaacaat
     1441 tattaacgcc cggttaccag gcaaagaggg gctgtggcag attcatctgc aggacggaaa
     1501 aatcagcgcc attgatgcgc aatccggcgt gatgcccata actgaaaaca gcctggatgc
     1561 cgaacaaggt ttagttatac cgccgtttgt ggagccgcat attcacctgg acaccacgca
     1621 aaccgccgga caaccgaact ggaatcagtc cggcacgctg tttgaaggca ttgaacgctg
     1681 ggccgagcgc aaagcgttat taacccatga cgatgtgaaa caacgcgcct ggcaaacgct
     1741 gaaatggcag attgccaacg gcattcagca tgtgcgtacc catgtcgatg tttcggatgc
     1801 aacgctaact gcgctgaaag caatgctgga agtgaagcag gaagtcgcgc cgtggattga
     1861 tctgcaaatt gtcgccttcc ctcaggaagg gattttgtcg tatcccaacg gtgaagcgtt
     1921 gctggaagag gcgttacgct taggggcaga tgtagtgggg gcgattccgc attttgaatt
     1981 tacccgtgaa tacggcgtgg agtcgctgca taaaaccttc gccctggcgc aaaaatacga
     2041 ccgtctcatc gacgttcact gtgatgagat cgatgacgag cagtcgcgct ttgtcgaaac
     2101 cgttgctgcc ctggcgcacc gtgaaggcat gggcgcgcga gtcaccgcca gccacaccac
     2161 ggcaatgcac tcctataacg gggcgtatac ctcacgcctg ttccgcttgc tgaaaatgtc
     2221 cggtattaac tttgtcgcca acccgctggt caatattcat ctgcaaggac gtttcgatac
     2281 gtatccaaaa cgtcgcggca tcacgcgcgt taaagagatg ctggagtccg gcattaacgt
     2341 ctgctttggt cacgatgatg tcttcgatcc gtggtatccg ctgggaacgg cgaatatgct
     2401 gcaagtgctg catatggggc tgcatgtttg ccagttgatg ggctacgggc agattaacga
     2461 tggcctgaat ttaatcaccc accacagtgc cagaacattg aatttgcagg attacggcat
     2521 tgccgccgga aacagcgcaa acctgattat cctgccggct gaaaatggat ttgatgcgct
     2581 gcgccgtcag gttccggtac gttattcggt acgtggcggc aaggtgattg ccagtacaca
     2641 accggcacaa accaccgtat atctggagca gccagaagcc atcgattaca aacggtaatt
     2701 tcctctgtgt tgaagatcca ctgacctgtg tcagtggcta ccgtgattca ttctcgccaa
     2761 catccgcaca ttcctccaac gccatgtgca aaaatgcctt cgcagcggca gtctgccagc
     2821 tatttttccg ccgcaacaaa accgccgttc tctccagtag tggcggggca agagaaatcg
     2881 ctttaagccc gtcatgttgt gtggcaatcg ctgctggtaa caatgtggaa agggaagtgc
     2941 ggcgaatcag ctccagaacc gcgctaattg agttcgcctc aatgaccacc tgtggatgta
     3001 gccccgcttt ctcgcagtag tggtcaattt gctctctggt ggcaaattcc gcgctgagca
     3061 ggaccagttt ttcatcatgc aagcgactca acgccacctg ttcacaggca gccagcggat
     3121 gatgtttcgc caccaccaac gctaaacttt ctgtcagtaa aggaattgcc tccagctccg
     3181 gcgaatgcac aggcgcgaag gcaatcccaa cgtccagctc gtcgcgacaa agcagatctt
     3241 cgattttctc ctgcgacatt tcctgtagct ggagcgtgat gccggggtag cgcgcataga
     3301 aatccgccat taaggggccg ataaagtagc tcgtaaaggt gggggtgacg gcgatacgca
     3361 gcgatcctcg cgtcagagcg gcaacatcat gaatcgcccg tttacccgct cccagttcat
     3421 gtaacgcccg gctggcgtac tgtcgccaga cttctcctgc atcggtgaga cgaatcgttc
     3481 gcccgctacg gtcaaacagc ggcacgccca aactctcctc taactggcga atctgctggg
     3541 aaagcgcagg ctgggagacg tgcaacgcac tggcggcacg ggtgaagctg ccatgttcag
     3601 ccacggcaag aaagtaattg atatgtcgag agagcattcg ccaacctata agtaaatcca
     3661 atagaactca tcataaatga gacttttacc ttatgacaat cggcgagtag tctgcctctc
     3721 attccagaga cagacagagg ttaacggtga aagagattat tgatggattc cttaaattcc
     3781 agcgcgaggc atttccgaag cgggaagcct tgtttaaaca gctggcgaca cagcaaagcc
     3841 cgcgcacact ttttatctcc tgctccgaca gccgtctggt ccctgagctg gtgacgcaac
     3901 gtgagcctgg cgatctgttc gttattcgca acgcgggcaa tatcgtccct tcctacgggc
     3961 cggaacccgg tggcgtttct gcttcggtgg agtatgccgt cgctgcgctt cgggtatctg
     4021 acattgtgat ttgtggtcat tccaactgtg gcgcgatgac cgccattgcc agctgtcagt
     4081 gcatggacca tatgcctgcc gtctcccact ggctgcgtta tgccgattca gcccgcgtcg
     4141 ttaatgaggc gcgcccgcat tccgatttac cgtcaaaagc tgcggcgatg gtacgtgaaa
     4201 acgtcattgc tcagttggct aatttgcaaa ctcatccatc ggtgcgcctg gcgctcgaag
     4261 aggggcggat cgccctgcac ggctgggtct acgacattga aagcggcagc atcgcagctt
     4321 ttgacggcgc aacccgccag tttgtgccac tggccgctaa tcctcgcgtt tgtgccatac
     4381 cgctacgcca accgaccgca gcgtaacctt atttttaaac catcaggaga tccaccatga
     4441 ttcagtcaca aattaaccgc aatattcgtc ttgatcttgc cgatgccatt ttgctcagca
     4501 aagctaaaaa agatctctca tttgccgaga ttgccgacgg taccggtctg gcagaagcct
     4561 tcgtaaccgc ggctttgctg gggcagcaag cgcttcctgc cgacgccgcc cgcctggtcg
     4621 gggcgaagct ggatctcgac gaagacgcca ttctactgtt gcagatgatt ccactgcgtg
     4681 gctgcattga tgaccgtatt ccaaccgacc caacgatgta tcgtttctat gaaatgttgc
     4741 aggtgtacgg cacaaccctg aaagcgttgg ttcatgagaa gtttggcgat ggcattatca
     4801 gcgcgattaa cttcaaactc gacgttaaga aagtggcgga cccggaaggt ggcgaacgtg
     4861 cggtcattac cttagatggt aaatatctgc cgaccaagcc gttctgacag ccatgcgcaa
     4921 tcatcaaaag acgttcacga tgctgctggt tctggtgctg attggtctta atatgcgacc
     4981 actgctcacc tccgtcgggc cactgctacc gcaattgcgc caggcgagcg gaatgagctt
     5041 tagcgtggct gccctgttga ccgctctgcc ggtggtcacc atgggcgggc tggcgctggc
     5101 cggaagctgg cttcatcagc atgtcagcga acgtcgcagt gtcgccatca gtctgttgct
     5161 gattgccgtc ggtgcattga tgcgtg
//
LOCUS       DAZFXT010000219         5046 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.219, whole genome shotgun sequence.
ACCESSION   DAZFXT010000219 DAZFXT010000000
VERSION     DAZFXT010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5046
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.219"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..924)
                     /locus_tag="ACHKW0_004784"
     CDS             complement(<1..924)
                     /locus_tag="ACHKW0_004784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6531 domain-containing protein"
                     /protein_id="HFO7477455.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRHFRLVLTTQAQRAEEARQQAISGGTEPSAFPDT
                     LPGYTEYGRDNGIRLSAVWLTHDPEYPENLPA"
     gene            1153..1761
                     /locus_tag="ACHKW0_004785"
     CDS             1153..1761
                     /locus_tag="ACHKW0_004785"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HFO7477456.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1859..3250
                     /gene="selA"
                     /locus_tag="ACHKW0_004786"
     CDS             1859..3250
                     /gene="selA"
                     /locus_tag="ACHKW0_004786"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HFO7477457.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            3247..>5046
                     /gene="selB"
                     /locus_tag="ACHKW0_004787"
     CDS             3247..>5046
                     /gene="selB"
                     /locus_tag="ACHKW0_004787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HFO7477458.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALNIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRG"
BASE COUNT         1085 a         1353 c         1508 g         1100 t
ORIGIN      
        1 ggcaggtaaa ttctccgggt attccgggtc gtgcgtcagc cacacggcag acagacggat
       61 gccgttgtcc cggccatatt cggtgtaacc cggcagggta tcaggaaaag cggacggttc
      121 cgtcccgccg gaaatggcct gctgccgggc ttcttctgcc cgctgcgcct gcgtggtcag
      181 taccagccgg aagtgacgcc cggcaccatc cgtcacgccg gtgatttcgc cgctgaattc
      241 accggcggct tcgcggtgga acgtctgtgt gcgcccgaag cggtccacca gcccggtcag
      301 tacccggtac ggcggcagcg gcgcaggcag cacctcatcc gcttccggca cccgctcaca
      361 ccagccgagc agccaccacg gcccctgcgg actgtttgtc gccagataac gatgcggact
      421 taagcggagt tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag
      481 tttcgccacg ccgccgcgca ccagccacag tgactcgctg cggctgtaac cgtcctcacc
      541 gggaaacagg tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt
      601 atcgcgcagc tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac
      661 cggcgcgggc gtttttgtcc ggtaactgct gtaggtgcgg gagaggatga acggcagcgg
      721 ggcgggcagg gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca gaggattgac
      781 cggatggccg gacgtcactc cgccggggca caccgaacag gccacgccgg tgggggcacc
      841 aatgcgcacc ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg
      901 acgcgctgcc ggttttccgc tcatctgctc atccttgttt tatcgggtta tttgtctgct
      961 tccaggcaaa accctacaga tttataagaa agccttaagt gagatatcca tcagatagaa
     1021 acaattagga aaatatttat atcaccatga taaatatgtg atttatttgg aagtaacaaa
     1081 ccatcaacat tttctgccct ttttcccatc accaggccat actgatattt ttcctcgcac
     1141 aaaggactcg gcatgaaact cgtcggtagc tacaccagcc cgtttgtacg caaactttct
     1201 attctgttgt tagaaaaggg cataactttc gaatttatta atgaactgcc ctataacgcg
     1261 gacaacggcg tggcgcaatt taacccgtta ggaaaagtgc cggtgctggt gaccgaagag
     1321 ggcgaatgtt ggtttgattc gccgatcatc gctgaatata ttgaattaat gaatgtcgct
     1381 ccggcgatgt tgccgcgcga tccgctggag tcgttgcggg tgcgcaaaat tgaggcactg
     1441 gcggatggca ttatggatgc cgggctggta tcggtgcgtg aacaggcgcg tccagcggcg
     1501 cagcagtctg aagatgaatt gttacgccag cgggagaaaa tcaaccgcag tctggatgtg
     1561 ctggaaggat atctggtcga tggcacactc aaaaccgata cggtcaatct ggcgactatc
     1621 gccattgcct gtgccgtcgg atatctcaat ttccgccgcg ttgcgccagg ttggtgtgtc
     1681 gaccgcccac acttagtcaa actggtcgaa aacctgttta gccgcgaaag ttttgctcgc
     1741 accgaaccgc caaaggcttg atgcgcgata tgtcctcctg acccatctca cgttacaatc
     1801 cgtggttatg ttaaacgccc ttctccgtgt gagagggcct tgatcagcca ggtttcctat
     1861 gacaaccgaa acgcgttccc tctatagtca acttccggct attgatcgct tattgcgcga
     1921 tagctccttc ctttctttgc gtgatactta tggtcacacc cgcgtggtgg aattgttgcg
     1981 tcagatgctc gacgaagcgc gagaagtgat tcgtggcagc cagacgctgc ctgcgtggtg
     2041 tgaaaactgg gcgcaagaag tcgatgcccg gttgacgaaa gaagcgcaga gcgcgctgcg
     2101 tccggtgatc aacctgacgg gaaccgtgct gcataccaac cttggacgag ctttacaggc
     2161 ggaagccgcg gtggaagccg ttgcgcaggc tatgcgttcg ccagtgaccc tcgagtacga
     2221 tctggacgac gccggacgcg gacatcgcga tcgggcgctg gcgcaactgc tgtgccgcat
     2281 tacgggggcg gaagatgcct gtatcgttaa taacaatgcg gcggcggtgt tattgatgtt
     2341 ggcggccact gccagcggga aagaggtggt ggtttctcgc ggcgaactgg tggagattgg
     2401 cggcgcgttt cgtattcccg atgttatgcg tcaggcaggc tgcaccctac acgaagtagg
     2461 gaccaccaac cgcacgcacg cgaatgatta tcgtcaggcg gtgaatgaaa ataccgcact
     2521 gttgatgaaa gtacatacca gtaactacag cattcagggg ttcaccaaag cgatagatga
     2581 agcggaactg gtggcgctcg gcaaagagct ggatgttccc gtagtgactg atttaggcag
     2641 tggctcgctg gtcgatctta gccagtacgg tttgccgaaa gagccaatgc cgcaggagtt
     2701 gattgcggcg ggcgtcagtc tggtgagttt ctccggcgac aagttgttag gcgggccgca
     2761 ggcaggaatt attgttggta aaaaagagat gatcgcccgc ctgcaaagcc acccgctgaa
     2821 gcgtgcatta cgcgcggata aaatgaccct cgcggcgctg gaagccacgt tgcgtcttta
     2881 tttacaccct gaagctctga gtgaaaaatt accgaccctg cgcctgctta cccgcagcgc
     2941 agaggtcatt caaatccagg cacaacgttt acaggccccc cttgccgcac attacggcgc
     3001 ggagtttgcg gtacaggtta tgccatgtct ttcgcagatt ggcagtggtt cgctgccggt
     3061 tgatcgcctg ccgagcgcgg cattaacgtt tacaccccat gatggacgcg gtagccacct
     3121 tgagtcatta gccgcccgct ggcgtgaatt gccagtgccg gtgattggtc gtatttatga
     3181 cggacgattg tggctggatt tacgctgcct tgaagatgag caacggtttt tggagatgtt
     3241 gttgaaatga ttattgcgac tgccggacac gttgaccacg gcaaaacaac cttattgcag
     3301 gcgattactg gcgtaaatgc tgaccgtctg ccggaagaaa aaaagcgcgg catgaccatc
     3361 gatctcggct atgcctactg gccgcagccg gatggtcgcg tgcctggttt tatcgacgta
     3421 cccggtcatg aaaagtttct ttccaacatg ctggcgggcg ttggtggtat cgatcacgcg
     3481 ctgttggtgg tggcgtgcga tgacggcgtg atggcacaga cccgtgagca tctggcgatt
     3541 ttgcagctga ccggtaaccc gatgctgaca gtggcgctga ccaaagccga tcgcgtggac
     3601 gaagcgcgtg ttaatgaggt tgaacgccag gtaaaggagg tgctgcggga atacggtttt
     3661 gctgaggcaa aactgtttat caccgcagca accgaaggtc ggggaattga tgccctgcgc
     3721 gagcatctgc ttcagttgcc ggaacgcgag cacgccagcc aacatagttt ccgcctcgct
     3781 attgaccgcg catttaccgt aaaaggtgcc gggctggtcg tcaccggtac ggcgttaagc
     3841 ggggaagtga aggtaggcga ttcactctgg ctgactggtg taaataaacc gatgcgtgta
     3901 cgtgcgctgc atgcgcaaaa ccagccaaca gaaaccgcca atgccgggca gcgtatcgcg
     3961 cttaacatcg cgggtgatgc ggaaaaagag cagattaacc gtggcgactg gctgcttgcc
     4021 gatgtgccgc cagagccgtt cacacgggtg attgtcgagc ttcaaaccca tacaccgctg
     4081 acccagtggc agccgctgca tattcaccac gccgccagcc acgttacggg acgcgtttcg
     4141 ctgctggaag ataaccttgc cgagctggtg ttcgacacgc cgctgtggct ggcggataac
     4201 gatcgcttag tgctgcgcga tatctcggcc cgcaacacgc tggcgggggc gcgtgtggtg
     4261 atgcttaacc cgccgcgtcg cggtaaacgt aagccggaat atctgcaatg gctggcgtct
     4321 cttgcacggg cgcagagcga tgccgatgcg ttatctgttc atctggaacg cggcgcggtt
     4381 aaccttgcgg atttcgcctg ggcgcgccag ctcaacggcg aagggatgcg cgaattgctg
     4441 caacagcctg gctatattca ggctggttat agcttgttga atgcgccggt tgccgctcgc
     4501 tggcaacgga aaattctcga tacattagcg acttatcatg agcaacatcg cgatgaacct
     4561 gggcctgggc gcgaacgtct gcgacgtatg gcgttgccaa tggaagatga agcgctagta
     4621 ctgttgctga ttgaaaagat gcgcgaaagc ggcgacatcc tcagtcatca cggttggctg
     4681 catctgccgg atcacaaagc gggcttcagc gaagagcagc aggccatctg gcaaaaagca
     4741 gagccgctgt ttggtgacga gccgtggtgg gtgcgtgatc tggcaaaaga gacgggtacc
     4801 gacgagcagg caatgcgcct gacgctacgc caggcggcgc agcaaggaat aattaccgcg
     4861 atcgtcaaag atcgttatta ccgtaacgat cggattgtcg aatttgccaa tatgatccgc
     4921 gatctcgatc aggagtgtgg ttcaacctgc gcggcggatt tccgcgatcg cttaggcgta
     4981 ggccgaaagc tggcaattca gattctggaa tattttgacc gcattggctt tacgcgtcgt
     5041 cgtgga
//
LOCUS       DAZFXT010000220         4892 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.220, whole genome shotgun sequence.
ACCESSION   DAZFXT010000220 DAZFXT010000000
VERSION     DAZFXT010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4892
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.220"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1735)
                     /locus_tag="ACHKW0_004788"
     CDS             complement(<1..1735)
                     /locus_tag="ACHKW0_004788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HFO7477459.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARISASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGDEIKQIV
                     EHSGLFLGDNAVRTLTGLIEKQHQQAQIISADNVQRLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMEL"
     gene            complement(1735..2805)
                     /locus_tag="ACHKW0_004789"
     CDS             complement(1735..2805)
                     /locus_tag="ACHKW0_004789"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HFO7477460.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            complement(2795..4246)
                     /locus_tag="ACHKW0_004790"
     CDS             complement(2795..4246)
                     /locus_tag="ACHKW0_004790"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HFO7477461.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSHLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            complement(4257..4703)
                     /locus_tag="ACHKW0_004791"
     CDS             complement(4257..4703)
                     /locus_tag="ACHKW0_004791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HFO7477462.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHKEFIHQLQQGDTDQVLALLNQTLSS"
BASE COUNT         1219 a         1280 c         1251 g         1142 t
ORIGIN      
        1 gtaactccat aaatgcctca tatcctgtta acccggcaat aacctctgcc ggatgctgac
       61 acaatacgct tgccagatag ctaaatattt ttagctcatg acgcgcatcg gcggcggcgc
      121 aggcgatcaa gacatggtta atgacttcgt tattcacctc aactggttgg gcgagggtaa
      181 taaaaaatcc acgaaaacgt cgctcctgtt cgctccagca atgtgggatg gcgaggcgat
      241 taacaatcag gttttcacct tcgccttcgc gggcgatgat gcgttgtgct tcatcggcgg
      301 taatatggtg ttgtgccact aattgcgcac aaatttgccg ggtaatatgt tgccagcttt
      361 caccgcgtac attcgaataa tgaaaacttc cgggggcaga gaaaaaacgc tccggctgtt
      421 ggcgaataaa tgccgtcgcc agaaaatgtt ttatttgttc gataccggca gccgtaatga
      481 tattttttac ggtgatgtaa ttatttaccg cgtcatccag taaataatgg ctgttgttaa
      541 tgatcaataa cggctcaatc tcttcgcgaa tggcaacaag ttcgcttaag ctacgggcaa
      601 taatcacccg acaatgtaaa acatcgcgct caatggcgag ctgattaata gtggcaatcg
      661 cgttctggtc cgagagcaaa atgatcggct ggcgttcgtt ttgatgccgt tccagcgcac
      721 aggcaaaata caaaccgatc agatcgctgt cgaaaagggg aatatcgagc tgttcacgca
      781 gtagcgtaat aaagtgcaga ctcatatcaa aggccgcagg ccaggcggct ttcaggttat
      841 tcatgctgct ttggcggtgc tcagcaatcc acaccggtgc ggcaaggcaa cgtaataaat
      901 gcccggtaat gttctcaacc agctgcgtat caataatatt caatgacgcg atgcccggca
      961 ccctttgcag caaccgctgc acattatcgg cagaaattat ctgcgcttgc tgatgctgtt
     1021 tctctatcaa acctgtcagg gttcttacag cgttatcacc aagaaacaga ccgctatgtt
     1081 caacgatttg ttttatctcg tcaccgggat attgcggcca ctcatcggtc agttgattac
     1141 gcagggcgta aatcgccaga atcaggctgg agagataatc accctgcatg agcggccagc
     1201 ggtgttggtt gtcgcaggcc gtggataaat gctgaaggtt gtctcgtgtt acgcccgcca
     1261 gcggaattaa aaacggatct ttgcgcagca agttcgccag caagataacg cgtttctctt
     1321 ctgtctcatc aatgaaatgg cccaaaccag ggcggctggc caggcaacaa gtgcgttcat
     1381 aacgctgttt taaccggggc agacgctctg cgacccacgt ttctggcaaa ttaagcgccg
     1441 aggcgagttg cgcacgggga gtaaaggtat tcagtaataa cagtgccagc agccgatcgt
     1501 cgttatcgtg cttttgcagc aactgaaaaa agctgcggcg ctcgaagatt tccagctgat
     1561 agcccgcact accactggcg gaaatgcggg ctttgccgtt aagggtaaag ttgagatagt
     1621 caatatcacg caggatggtc ctgcctgaaa cgccagtctg ttgcgccagc tcaccaggcg
     1681 tgcgcggctg ttgctccagc agatcgacaa tctttaactg gcgttcgttt agcattaatc
     1741 cacctgacgg aactggctaa acgcgttgac tttctccgcg gttaaggtgg tcagaaaatc
     1801 ccgtatcaac gtgagcagag catcgtaatc ggcttttgaa atcataccgc tgttagcgtg
     1861 cagatagcgg gttggcaggc acagcgcaac aaccggacgc ccgccgccca tcacgttgta
     1921 gcgcccgcca tccgtcgcac cggttttcat ggtggaaaat tgcagcggta aatcgttatg
     1981 tgcggcacag ctttttaacg ctgctaccag tttctggttg gggaagtagc gcttgtcaaa
     2041 cagcatcagc cccggcccct ggcccagttt cagcgggtat ttaatgttat caatgcccgg
     2101 aacatcgcct gcgacagcgg tatccagcac gatcactaca tccggtttaa tgtgttccgc
     2161 cgaggtttgt gccccgcgta gcccaacttc ttcttccacg ctgccgacgc catacagcgt
     2221 aatttcggga ttattcaccg tctgcaatag ttcagccatc attgcgcagc cgatgcggtt
     2281 atccagcgcc ttgccgacca ctttatcttc gccccagcag gcaaaattgg cttccgggct
     2341 aataaaatcg cccatcgcca caccgcgttt ttccacctca tcgcgactgt tcgcgccaat
     2401 atcaatgaac atctcatcaa atgacagcgg ttgttgcttt tgcttttccg ttaacgcatg
     2461 aggcgcgacg gaaccaatca caccagggat tttcactccc ttgtgtgtgc gtatggttac
     2521 ccggtggttg agcatcgact gattccacca gccgccaatg gtggtaaaac gcagaaaacc
     2581 gctctcgtcg atgtgggtga ccataaagcc gacttcatcc atatgcccga caacggcaac
     2641 ttttggacct ttattcccct tacgggcaac aaagctgccc agaccatcaa aggtgatttc
     2701 attaacgcaa ggttccagcg tgtttatcag gatgtcgcga acttcctgtt catcgccgct
     2761 gacggcgctg gcttcgcaca actgttgcag taactcaatg ttcatggaca agctcctgtt
     2821 gcgcggcttt cgctttgcgg cgtaaccaca atcctttcag gacgataatg aatgaaatgt
     2881 tcagcgcgag gccaatcgcc agcaccagat aaaacgaacc taccggcgac atcaggccga
     2941 tcagcgggtc aaagataccc aaaccaggcg ccagacgttt gattccgaaa gcgatcacca
     3001 gcacgccggt aatgccgccc gccagcgtat tggcggtaat catcggcaac ggagctgcca
     3061 gagcgtaagg aatagccggt tcggttgcga ctgtggcacc gaccacaatg gcactactgg
     3121 cagcctcttt ttcctgtcgg gtaaacagtt tgggcgcaat gaacgtcgca agacccgccg
     3181 cgacgggcgg catcaacgcg acaacgccga cgatggcgta ccagtcataa atgtgttttt
     3241 ccagcagtga gaagcagaag aaccaggcgg ttttattgat ggggccgccc atatcgaacg
     3301 ccagcatcgc accgaccaga aacgctgccg caaacttcat tgatggagga atggtgttca
     3361 ggaagtggag cagcccgccc atgaggtcgg acatcaccgg cccgataacg tagtaagtga
     3421 gcacgccgaa aaccagcaga gtaacgaacg ggatcagcat tgaaccgagc agcggttgta
     3481 atgctttgcc aaggcgcact ttgcggaacc agaagacgaa atagccaatc gccagcccca
     3541 gcaccaccgc gccgaggaat cccgcacccg actgggtgcc gagcagcgct ttgtcgttcg
     3601 ccagatagca aaccagaaac gccggagcga aagccggttt atcggcaatc gacgaggcaa
     3661 tataagcgcc catgatgggg atcataaagg tgaagccgag gtaaccgatg gattcaacta
     3721 cccaggtaaa cgacggcgca cctttcgaca tatcggtata tggcaaaccg aactgcacca
     3781 gcatgttggc aagcgccacc agaataccgc cgccgatgac aaacggcagc gccgcagaga
     3841 cgcccgccat caggtggctc atcacgctgc cgctttgcac ttcctgtttg ccaagcttca
     3901 cgccgctatc tgcagcaaaa agatgcgagt ttgttggtaa ttcactgaaa atctgatcga
     3961 tatttttcag cgcctgggag atggcaatct cataaacctt cttcccggca aaccgcgcgc
     4021 gatcgtcacc gctcaggcca cgcccggtag cgagaatgac gtaatcggca gcggcaatct
     4081 cttcgctgga taagcggttt tcgacgccgc ttgatccttg agtttcaact ttaatggtat
     4141 gaccgagaga acgggctttc tgttccaggg cttccgccac catgtaggtg tgagcgatcc
     4201 cggcggggca gttggtgatc gcgacaatac gtaaggatga ctccatcgcc acttccttat
     4261 gagctgaggg tttgatttaa caaggcaagc acctggtcgg tatcgccctg ttgcagttga
     4321 tgaataaatt ccttgtgaat aattttgcga cacagtgtgc cgatgatttt gacctgatcc
     4381 tcttcgccgc tttgcggcac gccgaggcag atccagcaat tgacgtcttc gccatcgctg
     4441 gctttccagt caatagcctg cgctttgcgg gcgaataata cgaacggttg tttaacgcag
     4501 gcgcttttac cgtgcggcac ggcgacaccg gaaccaaatc cagtagagtg cattttttcg
     4561 cgcagcagca gggtttgcag gaactggtgt gagtcggtga taaaaccgtt ttgtaacgct
     4621 attgtcgcca actgcttcag aacggaataa gcgccattgc cctgaatatt caggtcaata
     4681 cagcttgcag taagagctgc cattaatcgg actccataaa atatatttta atcaattgta
     4741 atggtggagc gattaagtga tgagaggcga tatgtcggta aacaatgaca atttgagaca
     4801 gagtgaaagg tcagatttgc ggagtaatgc acataatggt tatttaaata aaccacatga
     4861 atcattaatg ggttattcat tatttttgtg at
//
LOCUS       DAZFXT010000221         4824 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.221, whole genome shotgun sequence.
ACCESSION   DAZFXT010000221 DAZFXT010000000
VERSION     DAZFXT010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4824)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4824)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4824
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.221"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            121..1095
                     /gene="hemB"
                     /locus_tag="ACHKW0_004792"
     CDS             121..1095
                     /gene="hemB"
                     /locus_tag="ACHKW0_004792"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HFO7477463.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1201..2052)
                     /gene="tauD"
                     /locus_tag="ACHKW0_004793"
     CDS             complement(1201..2052)
                     /gene="tauD"
                     /locus_tag="ACHKW0_004793"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HFO7477464.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2049..2876)
                     /gene="tauC"
                     /locus_tag="ACHKW0_004794"
     CDS             complement(2049..2876)
                     /gene="tauC"
                     /locus_tag="ACHKW0_004794"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HFO7477465.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2873..3640)
                     /gene="tauB"
                     /locus_tag="ACHKW0_004795"
     CDS             complement(2873..3640)
                     /gene="tauB"
                     /locus_tag="ACHKW0_004795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HFO7477466.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSILLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3653..4615)
                     /gene="tauA"
                     /locus_tag="ACHKW0_004796"
     CDS             complement(3653..4615)
                     /gene="tauA"
                     /locus_tag="ACHKW0_004796"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015411 - ABC-type taurine transporter
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HFO7477467.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDYAEKHP
                     EVVKAFAKSAIDAQQPYIANPDVWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1107 a         1400 c         1241 g         1076 t
ORIGIN      
        1 aaagatgtcc tggtctctct ttcattctct tcttgtcaac atcacgacaa ctttcgtaaa
       61 acactcctgc cccgcttcag gtatactgtg caccttaatt ccacaaacat caggcagacc
      121 atgacagact taatccaacg ccctcgtcgc ctgcgcaaat ctcctgcgct gcgcgctatg
      181 tttgaagaga caacacttag ccttaacgac ctggtgttgc cgatctttgt tgaagaagaa
      241 attgacgact acaaagccgt tgaagccatg ccaggcgtga tgcgcattcc agagaaacat
      301 ctggcacgcg aaattgaacg catcgccaac gccggtatcc gttccgtaat gacttttggc
      361 atctctcacc ataccgatga aaccggcagc gacgcctggc gggaagatgg actggtggcg
      421 cgtatgtcgc gcatctgcaa gcagactgtg ccagaaatga tcgtcatgtc agacacttgt
      481 ttctgtgaat acacttctca cggtcactgc ggtgtgctgt gcgagcatgg cgtcgacaac
      541 gacgcaactc tggaaaattt aggcaagcaa gccgtggttg cagctgctgc aggcgcagac
      601 ttcatcgccc cttccgccgc gatggacggc caggtacagg cgattcgcca ggcgctggac
      661 gctgcgggat ttaaagatac ggcgattatg tcgtattcga ccaagttcgc ctcctccttt
      721 tatggcccgt tccgtgaagc tgccggaagc gcattaaaag gcgaccgcaa aagctatcag
      781 atgaacccaa tgaaccgtcg cgaggcgatt cgtgaatcac tgctggatga agcccagggc
      841 gcagactgcc tgatggttaa acctgctgga gcatacctcg acatcgtgcg tgagctgcgt
      901 gaacgtactg aattgccgat tggcgcgtat caggtgagcg gtgagtacgc gatgattaag
      961 ttcgccgcgc tggcgggtgc tatagatgaa gagaaagtcg tgctcgaaag cttaggttcg
     1021 attaagcgtg cgggtgcgga tctgattttc agctactttg cgctggattt ggctgagaag
     1081 aagattctgc gttaattttt ttcctctcag aaagggggag ttaacctgtt gaaagtacgg
     1141 tgaacgcccc ctcaccctaa ccctctcccc aagggggcga gggaaccgtc cgcactcgta
     1201 tcaccccgcc cgataaaacg gtttatcccc aaggatcgtc gcccgatgca taatccgccg
     1261 ctgtggcagg taatcggcat tggcatagtg ctgagtcacg cggttatccc aaatcgcaat
     1321 atcattcggt tgccagcgcc aacgcacctg aaactccggt ttagtgatat gggcgaataa
     1381 aaagcctaac aaggcttcgc tctctttctc gctgacatcg acaattcgcg tagtaaagcc
     1441 ttcattcaca aacagcgcct gtttaccgct caccggatgc gttcgcacca ccggatgtag
     1501 caacggagga tttttcgcga ccgcctcgcg ccagcgttga tgttcctcct cggttttgcg
     1561 gtatttgtat tccgggaacg atttacggaa atcatgctcc gcacgcagcc cgctcagcag
     1621 ctggcggaag ggaacagaga gcgcctcata ggccgcaata ccgctggtcc agagcgtatc
     1681 accgccggtc gaaggtaact ctttagctgc cagaatcgcc cctgcgggtg gcgtttcaat
     1741 aaatgtcaca tcggtatgcc agttgtcgtt atccggcgga ttatcgttat gggtatccag
     1801 cacgatgatc tcgtcaaccc cttcggcatg agggtaaaca gggtgaatat gcagttcgcc
     1861 aaaacgctgg gccagcgcgc gttgctgctg cggcgtaata gtttgatcgc gcagaaacac
     1921 cacctgatgg cgcagcaccg catggtaaag ctgttcaaac tgattatcgc ttaacgggcg
     1981 cgtcaggtcg gcacccgaaa tttgtgcgcc gatatacggc cccagcgggg taatgctcag
     2041 acgttcactc attgtacttc tccatgccag ggcgtcagac ggcgctgtaa cgcgcgcaaa
     2101 cccagttcta aaagaaaggc gataatcgca atcaccgcga ttcccgccag caccacgtca
     2161 gtcgcgagaa attcacccgc tgactgaacc ataaatccta aaccgcgcgt cgcggcaatc
     2221 agctccgccg ctaccagcgt agaccagccc acacccagac caatgcgtaa tccggtgagg
     2281 atttccggca gcgcgccggg caaaatgaca aaccacagca cctgcgcacg gctggcaccc
     2341 agcgactggg cggcacgaat gcgaacctgc tgcgcgcttt tcacccccgc cagcgccgac
     2401 atcgccaccg gtgcaaaaat cgctaaatag atcagtaaga tcttcgaggt ttcaccaata
     2461 ccaaaccaga tcaccatcag cggcaaataa gccagcggcg gcacgggacg ataaagttcg
     2521 attaccggat ccagaatgcc gcgcaccgtc gggcttagcc ccatggcgat cccgaccgga
     2581 ataccgatca ccaccgccgc gagtagcgcc agcacaatgc gcgtcaggct ggctgccaga
     2641 tgctgccaca gcgtggcgtc cataaatccc tgcggtccgg caatggtgag cagttttgcc
     2701 agtacttgtt gcggcggcgg caaaaacagc gggctaatca gttgcagcgc cgccaccgtc
     2761 caccatacag tgagtaaaac cgctaacgtg ccaatgctta aggtcacctg acgcgagagc
     2821 ggccagcgcc atttcagccg ccgcgaatgc agtttttcat taatgagcac actcatgaga
     2881 acgcctcccg ttgctcaaat acgcggctta aaacatattc gcgcatggcg atgaattgtg
     2941 gatcggactt gatgctgcgg ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg
     3001 gcagccgctc cagcacacgg ccagggccgg atgaaagcag aaccagttca gtcgccataa
     3061 acaccgcttc ttctatatcg tgggtaatca acagcacctg cttgcccgtc tcctgccaga
     3121 gtttcagcag cagagtttgc atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt
     3181 cgtcgagtaa taacagctgg ggattcgccg ccagcgcgcg ggcaatcccc acccgctgac
     3241 gttgaccacc ggaaagctgc cagatgtagc gtttttctgc gccttccagc cccacttttt
     3301 tcagcatctg gtgcgcgatt tccagtcgct gcattttctc tatacctgcc aattgcaggc
     3361 cgaacgccac gttgtcctgt acattgcgcc acggtagtag cccttcattc tgaaaaacta
     3421 cgccacgctc tgctcccggt ccctcaatac gcttacccgc cagtagaatg ctgccatgct
     3481 gataaggcac aaaaccggca atcagattca gcaacgtggt tttaccgcag ccggacggcc
     3541 ccagcaccac cagtagctcg ccgctttcca gcgtcaggtt gatatcttcc agtgccggtt
     3601 tgccgccata atcggcgtaa agatgagaga tttgcagcat ctgcgcctcc ttttattgca
     3661 cgaagcgcga ggtaacgtac tggctgtaat cattcgctac agccgggacc ttgccctgct
     3721 ctttcaaaaa ctgcgcggtg tcgatgatcg ctttgttcac cggtccggtc agttctgccg
     3781 tttgttgctg cggcgtcaga taggtattcc ccttcaccag ccccggaacg tcaccttcag
     3841 gcacgccgct taaacgcgcc agtttgctga tgttttccgg ctgtttcagc cacacgtctg
     3901 ggttagcaat gtacggttgc tgagcatcga tggcgctttt agcgaacgct ttcacgacct
     3961 caggatgttt ctcggcataa tctttgcgca ccacccagac gtccagcgtt ggcgcgcccc
     4021 actgcccgac ctgttcagaa tcggtcaaca ccttgccgtc tttttccagg gcgttaaccg
     4081 ccggtgccca gacataagca ccatcaatat ctccccgctg ccaggcagcg ataatcgcgg
     4141 gcggctgcag gttcacaatc tccacttgcc cgggtttaat gccccagtgt ttcagcgccg
     4201 ccagcaggct gtagtgggtg gtggagataa acggtacggc gatgcgtttg ccaatcagat
     4261 cttccggttt gctgatagtt ttctttacca ccagcgcttc ggagttaccc agttttgacg
     4321 ccagcaagaa gacttcaatc ggcacctgtt ggctggctgc aaccgctaac gggctggaac
     4381 cgaggttgcc gatttgcaca tcgcctgaag ccagcgcccg cacgatgctg gctccgctgt
     4441 caaacttacg ccagtccacg ctcgccccgc tctctttagc aaaggtgttg tcggcctgag
     4501 ccactttcgc cggttcggct gaggtttgat aggccacggt gacgttcacc gcctgtgcct
     4561 gaaaagcgat gaatgccagt gcggctagaa gtgtgttacg cgatgaaatt gccatgattg
     4621 tctgctcccc tgtcttgtta tgggagcagt attcaggaat aaaaacattc attaaaagaa
     4681 ttagtcgtta tcgcacagat gattttattc ttagcaaaaa aacggctaag gaaagattaa
     4741 atatgcaatt actccgttct aacaattggg aaaattacta cacttcattt tagtgatgca
     4801 taaacaacca cattacgaga ttta
//
LOCUS       DAZFXT010000222         4798 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.222, whole genome shotgun sequence.
ACCESSION   DAZFXT010000222 DAZFXT010000000
VERSION     DAZFXT010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.222"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            581..1144
                     /locus_tag="ACHKW0_004797"
     CDS             581..1144
                     /locus_tag="ACHKW0_004797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HFO7477468.1"
                     /translation="MYNANPNYEMDFMILKDVNEHMEGLFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCRHSMCIEMHRLLSETQTMLAGYYWVMEYTSNKGLHIHFIGYLDGQRHNKS
                     YRISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILRRSRLPEKSNRGRPRHN"
     gene            1299..3659
                     /locus_tag="ACHKW0_004798"
     CDS             1299..3659
                     /locus_tag="ACHKW0_004798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HFO7477469.1"
                     /translation="MVHKSDSDELAALRAENARLVSLLEAHGIEWRRKPQTPVQRVSV
                     LSTDEKVALFRRLFRGRDDVWALRWESKTSGKSGYSPACANEWQARICGKPRIKCGDC
                     AHRQLIPVSDLVIYHHLAGTHTVGMYPLLEDDSCYFLAVDFDEAEWQKDASAFMRSCD
                     ELGVPAALEISRSRQGAHVWIFFASRVSAREARRLGTAIISYTCSRTRQLRLGSYDRL
                     FPNQDTMPKGGFGNLIALPLQKRPRELGGSVFVDMNLQPYPDQWAFLVSVTPMNVQDI
                     EPTILRATGSIHPLDVNFINEEDLGTPWEEKKSSGNRLNISIAEPLKITLANQIYFEK
                     AQLPQVLINRLIRLAAFPNPEFYKAQAMRMSVWNKPRVIGCAENYPQHIALPRGCLDS
                     VLSFLRDNNIAAELIDKRFAGTECNAVFMGNLRAEQEEAVSALLRYDTGVLCAPTAFG
                     KTVTAAAVIAKRKVNTLILVHRTELLKQWQERLAVFLQAGDSIGIIGGGKHKPCGNID
                     IAVVQSISRQGEVEPLVRNYGQIIVDECHHIGAVSFSAILKETNARYLLGLTATPIRR
                     DGLHPIIFMYCGAIRHTAVRPKESPHNLEVLIRSRFTSGHLPSDARIQDIFREIALDH
                     DRTVAIAEEAMKAFGQGRKVLVLTERTDHLDEIASVMNSLKLSPFILHGRLSKKKRAM
                     LISGLNALPPDSPRILLSTGRLIGEGFDHPPLDTLILAMPVSWKGTLQQYAGRLHREH
                     TGKSDVRIIDFVDTAYPVLLRMWDKRQRGYKAMGYRIIADGDESVI"
     gene            complement(3821..3997)
                     /locus_tag="ACHKW0_004799"
     CDS             complement(3821..3997)
                     /locus_tag="ACHKW0_004799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477470.1"
                     /translation="MTEILRIAQTYEPWLDFWELVVQNVSTIASHTATNRPLPEAIMA
                     NYQLDEQLLEGCRP"
     gene            complement(4135..4377)
                     /locus_tag="ACHKW0_004800"
                     /pseudo
     CDS             complement(4135..4377)
                     /locus_tag="ACHKW0_004800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004389801.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4416..>4798)
                     /locus_tag="ACHKW0_004801"
     CDS             complement(4416..>4798)
                     /locus_tag="ACHKW0_004801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7477471.1"
                     /translation="LKSLESWLREKMKTLSRHSELAKAFAYALNQWPALTYYANDGWV
                     EIDNNIAENALRAVSLGRKNFLFFGSDHGGERGALLYSLIGTCKLNDVDPESYLRHVL
                     GVIADWPVNRVSELLPWRIALPAE"
BASE COUNT         1209 a         1008 c         1213 g         1368 t
ORIGIN      
        1 ttagtggaag ttatgtgatc tgttatgttg tcttgtacgc acttaacact ggttacgtac
       61 cggaacacta ttagagtatg ttatggctct gttacagttt ataggtatga aaaaatataa
      121 tctttcctgt atgaaataat attcttatgg tgtgtggggt atggtctgta taccatcatt
      181 tttatggtga agttaaatat ctgtagaggc ctcaagctgg gtcgctgttt ttacttaatg
      241 gtacagaagt attaatagcg gtgttgcctg tggctacccg atactggctt ttgtgaatgt
      301 cagattactt cctgtaagtg attgaatggt acttccttaa actcaattct atgaggtgtg
      361 tttttaccat tgtaattcgt actggtcctc agtttaagtg agtcttactg tactctgaaa
      421 tattacctct ttggagtaat gaggtaatac gaaacagttt tatcaggttg tctgtgctgc
      481 tggttacctc gtttattaat gccgcggtac ggcgtttctg tactctcagt atttatgtaa
      541 ttccaccttc ctgtaacttt tataaagtaa ggactataaa atgtataatg ctaatcccaa
      601 ttacgaaatg gacttcatga ttctgaaaga cgtgaatgaa cacatggaag gtctgtttca
      661 acgtttttct aaactgttac ctttcagaat tgattttgcc tacaggaaag acacgcccag
      721 ttttggtcat tcctgtaggc acagtatgtg tattgaaatg caccgcttac ttagtgaaac
      781 ccaaacgatg ctggcgggat attactgggt aatggaatat acgtcaaaca agggacttca
      841 tattcatttt atcggctacc tggatgggca acgtcacaac aaatcttatc ggatatccag
      901 gcaactggga gatatctgga ggcggatcac ggaaggggag ggttattttc acctgtgcag
      961 ggctaaagac aaatacccgg tgcgtatcga tcacgtaatt cactatagtg ataaatctgc
     1021 ggtagacgac ttacgttacg cccttagcta tctggccaaa caggatcaga aagaacacgg
     1081 tattattctg aggcgtagca ggcttcccga aaaaagtaac cggggccgac cacgacataa
     1141 ctgaattaca gctgtattag gtattagaat taagtttctg taagttgttg ggaggtgctt
     1201 gagttaatag gcagaagagg gcggggcgct ttgtcatcag tgtaggggac ccgaatttct
     1261 gccgtggtgc ctcagtggat cggatacagg taacgaatat ggttcataaa tctgacagtg
     1321 atgaattagc tgcattgagg gcagaaaatg cccgtctggt ctcattactt gaagctcatg
     1381 ggattgaatg gcgacgtaaa ccgcagactc ctgtgcagcg cgtttctgta ttatccaccg
     1441 acgagaaggt tgcattattt cgtcggttgt ttcgtgggcg tgatgatgta tgggcactta
     1501 gatgggaaag taaaaccagc ggcaaatcag ggtactctcc tgcctgcgct aacgaatggc
     1561 aggcgagaat atgcggtaaa ccccggataa aatgtgggga ctgcgctcac cgtcagttga
     1621 ttcctgtatc cgatctcgtc atctaccacc atctggccgg tacccatact gtcgggatgt
     1681 atccgttgct ggaagatgat tcctgttatt ttctggcagt tgattttgat gaagctgaat
     1741 ggcagaagga tgcatccgca ttcatgcgat cctgtgatga gctgggtgta cctgctgcgc
     1801 tggaaatatc ccgttcacgt cagggggcgc atgtctggat attttttgcc tcacgagttt
     1861 cggcccgcga agctcgccgt ctggggactg ctattattag ctatacgtgt agtcggaccc
     1921 gacagctgcg attggggtct tatgaccgat tatttcctaa tcaggatact atgccaaaag
     1981 ggggatttgg taatctcata gcgttacctc tgcaaaaaag accgcgtgaa ttggggggaa
     2041 gcgtttttgt tgatatgaat ctccagcctt atcctgatca gtgggctttt cttgtatcgg
     2101 tgaccccgat gaatgtgcag gatattgaac cgacgatatt acgggctaca gggagtatcc
     2161 atcctctgga tgtgaatttt atcaacgaag aagacctggg tacgccgtgg gaagagaaaa
     2221 aatcatcagg aaacagactg aatatttcta ttgcagaacc gctgaaaatc acgctggcaa
     2281 accagatcta tttcgaaaaa gcgcaattac ctcaggtgct gattaaccga cttattcggc
     2341 tggcagcatt tccgaaccct gagttttata aggctcaggc aatgcgtatg tcagtctgga
     2401 ataagccccg tgttataggt tgtgcggaga attacccgca acacattgcg ttgccccggg
     2461 gatgtctgga cagcgtatta tctttcctta gggacaacaa tattgctgca gaattaatcg
     2521 ataaacgatt tgccgggacg gaatgtaatg ccgtttttat gggaaacctc agagcggagc
     2581 aggaagaggc cgtttcggca ttactccgtt atgacactgg tgtgctttgt gcgccaacgg
     2641 cttttggtaa gacagttacc gcagcggcag tgattgccaa gcggaaagtg aatacactga
     2701 tactggtaca ccggactgaa ttgctgaagc agtggcagga gcgtctcgcg gtgtttctgc
     2761 aggccggtga cagtattggt attatcgggg gaggtaaaca taaaccctgt ggcaatattg
     2821 atattgcggt ggtgcagtcc atatccagac aaggagaagt tgaacctctg gtcaggaatt
     2881 atgggcaaat cattgtggat gagtgccatc atattggcgc ggtttcattt tctgcgattc
     2941 tgaaggaaac gaatgccaga tatctgcttg gcctgacggc aacaccaatc cgacgggatg
     3001 gtctgcatcc cattattttt atgtactgtg gtgccattcg ccatacagcg gtccgcccga
     3061 aggaaagccc acataatctg gaggtactga tccgttcccg ttttacatct ggtcatttac
     3121 catcggatgc gagaatccag gatattttca gagaaattgc tctggatcat gacagaacgg
     3181 tggcgatagc tgaagaagcc atgaaagctt tcgggcaggg gcgaaaagtt ctggtactga
     3241 ctgaacgtac agatcatctg gatgagatag catcagtgat gaattcactg aaattgtctc
     3301 cctttattct ccatggtcga ctatcgaaaa aaaagcgtgc gatgctgata tccgggctga
     3361 atgctcttcc tcccgattct cctcgaattt tgttgtcaac aggcagactt attggtgagg
     3421 gatttgacca ccctccgctg gatacgctga ttcttgccat gcctgtgtca tggaaaggga
     3481 cattacagca gtatgcaggg cgtcttcaca gagagcatac cggtaaaagc gatgtcagga
     3541 tcattgattt tgtggatacc gcgtatcctg tgctgctcag aatgtgggat aaacgtcagc
     3601 ggggttataa agcgatgggg tacaggatta tagctgacgg cgatgaatca gtgatttaat
     3661 tttaaggagt actttaccct ttttaaataa tttgagctcc ccgggttgtt cgggcaacaa
     3721 agagtcccag tatacaggct tcaggaatat gttgtagtat caggcttttc atcgctttaa
     3781 aatgactacc ggcagtgaga acgtcatcaa aaataacaat ctagggcctg cacccttcca
     3841 gtaattgttc atccagttgg tagtttgcca taatcgcttc tggtaagggc cggtttgttg
     3901 cggtatgaga ggctattgtt gatacgtttt gcacaaccag ttcccagaaa tccagccatg
     3961 gttcgtaggt ttgcgcaatt cgcagaattt ctgtcatacg gtcatcgtaa gcggcatctg
     4021 tacggcattt ggatggtggt acgggaacaa atgtgtattc ctcaagatta ccgatatttg
     4081 atatgagcat ctgagcaaca tactcaatag cagccttttt atagtggtaa tcaggctacg
     4141 aaagtacagc gttttataaa ttgtctcagg tgatattcgc agcgtttttt gacgtggttt
     4201 tgttcgcctt aaccatcctg atatttgctc tggagaccat ttcatctcca gcttttccag
     4261 aacaagcttt cgcaatggta aattttgatc cagtaagcac ggttttggcc ttttcgccat
     4321 tctgttggct cggttattag catcaacagc tttgtaatag cgtctgcccc gattacgcgt
     4381 aagcgtacag cgagggccgt attgacgggg atgtgttatt cagctggcag tgctatgcgc
     4441 cacggaagca gttcgctgac ccggttgacc ggccagtctg ctatgacgcc aagcacatgg
     4501 cgaaggtagc tttctggatc cacgtcattc agtttgcacg tcccgatcag gctgtacagt
     4561 agcgctcccc gctcaccacc atgatcagag ccgaagaaca ggaagttttt acgacccaga
     4621 ctgaccgccc gcagggcatt ttcagcgatg ttgttgtcga tttccaccca gccatcgttc
     4681 gcatagtacg tcagtgccgg ccactggtta agtgcgtacg cgaacgcctt cgccaactct
     4741 gagtgtcgcg acagggtctt catcttttca cgcaaccagc tttccaggga tttcaaca
//
LOCUS       DAZFXT010000223         4664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.223, whole genome shotgun sequence.
ACCESSION   DAZFXT010000223 DAZFXT010000000
VERSION     DAZFXT010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.223"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..410)
                     /locus_tag="ACHKW0_004802"
     CDS             complement(<1..410)
                     /locus_tag="ACHKW0_004802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO7477472.1"
                     /translation="MENNDKFLSQDLLESYAIRLLSGPLNGCEYEILNGRLLVIIGND
                     VSLGRSDAFSELPENTIVVPYGELTGSFEIIITTDPDLVVTFRELTAQEPEDRTLTLN
                     QQIEVLGLKFAVKEKNEVWQYSLPGIIENNIIST"
     gene            668..1168
                     /locus_tag="ACHKW0_004803"
                     /pseudo
     CDS             668..1168
                     /locus_tag="ACHKW0_004803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311747.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            1170..1463
                     /locus_tag="ACHKW0_004804"
     CDS             1170..1463
                     /locus_tag="ACHKW0_004804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HFO7477473.1"
                     /translation="MSLVGYNSNIIITIALISLLSACTRENSLKENQNKRSGESQAIV
                     DARQFFSEHPEYISSAELEQKLYREFAQVTTVPAYKEMSMYQLLIISQDRLTH"
     gene            complement(1524..2644)
                     /locus_tag="ACHKW0_004805"
                     /pseudo
     CDS             complement(1524..2644)
                     /locus_tag="ACHKW0_004805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(2653..3420)
                     /gene="sctT"
                     /locus_tag="ACHKW0_004806"
     CDS             complement(2653..3420)
                     /gene="sctT"
                     /locus_tag="ACHKW0_004806"
                     /inference="COORDINATES: protein motif:HMM:TIGR01401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HFO7477474.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWMFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNFPKLEPLFSYINNIMTHTIVYASPVIAVMLGGEAVLGLLACYASQLNAFAISLTV
                     KSALAFLILIIYFAPILAERVMPLSFFPEQLQLYIEK"
     gene            complement(3422..3682)
                     /locus_tag="ACHKW0_004807"
     CDS             complement(3422..3682)
                     /locus_tag="ACHKW0_004807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7477475.1"
                     /translation="MDDIVFAGNRALYLILVMSAGPIEVATFVGLLVGLFQTVTQLQE
                     QTLPFGVKLLCVSICFFLMSGWYGEKLYSFGIEMLNLAFARG"
     gene            complement(3692..4357)
                     /locus_tag="ACHKW0_004808"
     CDS             complement(3692..4357)
                     /locus_tag="ACHKW0_004808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HFO7477476.1"
                     /translation="MSNSISLIAILSLFTLLPFIIASGTCFIKFSIVFVIVRNALGLQ
                     QVPSNMTLNGVALLLSMFVMMPVGKEIYNNSQNENLSFNNVASVVNFVETGMSGYKSY
                     LIKYSEPELVSFFEKIQKVNSSEDNEEIIDDDNISIFSLLPAYALSEIKSAFIIGFYI
                     YLPFVVVDLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLILQYFDLS
                     INP"
     gene            complement(4347..>4664)
                     /locus_tag="ACHKW0_004809"
     CDS             complement(4347..>4664)
                     /locus_tag="ACHKW0_004809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HFO7477477.1"
                     /translation="EDFETDDFDIKKNESEIYDENDEQMINSFEELPVKIEFVLGKKI
                     MNLYEIDDLCAKRIISLLPESEKNIEIRVNGALTGYGELVEVDDKLGVEIHSWLSGNN
                     NVK"
BASE COUNT         1580 a          916 c          711 g         1457 t
ORIGIN      
        1 tttgtagaaa taatgttatt ttcaataatg cctggcaatg aatactgcca aacttcattt
       61 ttttctttta cagcaaattt gagacctaaa acttctattt gctgattcaa tgttaacgtt
      121 ctatcttcag gttcttgtgc tgttaattct ctgaatgtta ctaccagatc agggtcggta
      181 gtaattatta tctcaaaact acctgtaagc tcgccataag gaacgactat tgtgttttct
      241 ggtaactcag aaaaagcatc acttcttcct aacgaaacat cattaccaat gataacaagt
      301 agacgcccat taagtatttc atactcgcat ccattcaatg gtccgctcaa caaacgaatg
      361 gcataagatt ccaataagtc ttgtgataag aatttatcat tattttccat gaataccacc
      421 cgctaattcc ataagctcta gtttagagta gtgtaacaag acggaaattt catgaccaaa
      481 ttatgatgac acagaataaa aaaattataa tttcgagaac tattttaaat gcctctatgc
      541 ataactttat ttacaattta aggtataaac cctattccct caatgttaag caaatataac
      601 aatcactctt attttaaatt ttacacgtaa tatttaccct aaatgatttt agagtacagg
      661 tgggcatatg caagtctttt ccagtgatgt ctacttcact gtaggcacta atgcattatt
      721 agcatcccaa aaagaatatt acagcgattt agttgcgttg gttgatcttg gacattcgtt
      781 cgttgttatt gatgagcatc aacatcgaaa tctcaaacca aatacaaaac ctgtaaacat
      841 ccttttgaga aacaatttca tcaggatcaa taagaatatt acattatctg atttaacgca
      901 tttcctggtt tcaaatcttc atacacaaaa tgtgtattcc acacaagaac cacttatcca
      961 tgatgagata gatatcctgc ggttatgtgt ttcttactcg ttaaaacaaa tagccattat
     1021 taaaggaatt gattataaaa caatatcata tcacaaaata agagctctaa ataaacttaa
     1081 cataaaaggt actgtttagt tgtttatagc actctgtgaa tgggataaac attattttaa
     1141 actccaatct tgcgtaagtg aaagttgata tgtcattagt aggatataat agtaacatca
     1201 taataaccat cgcgctgata tcgttgctct ctgcatgcac cagagaaaat tcattaaagg
     1261 agaatcaaaa taaaagaagt ggcgaatccc aagcgatagt agatgcaaga cagttcttta
     1321 gcgaacaccc ggaatatata tcatcagcag aactggagca aaagctatat cgcgaatttg
     1381 cacaggttac tacagttcca gcttataaag agatgagtat gtatcaattg cttatcattt
     1441 cacaagatcg tttaactcat taaataagga tctacacgct ttttgcgtgt agatccttat
     1501 aaatttctga ctacgacaga tgtttaattt tttaaattat cgtctttatt ctcgattttt
     1561 gtgtcttcac cctcaatgaa tttatcttct gaagagagct gttcatcttg aacaggttgc
     1621 ccagcattct ccacatcttc aagccacagc agaagacgta atatttcatc tatattttcg
     1681 aagctaacat aatcgtagcg acgatgggta gcataatttt ttcgtgctaa ttttttatct
     1741 gtgataattg gtattccgat ttcctttgca tatttcctta cgcctaaggc cacctcatta
     1801 gttactctta cagaaatcaa tggaataggt gaaagatgtg gcttaaagta tatccctatt
     1861 gcaatgtgag tagggttgga aatcatcaaa cgactattac tgacatcaga cttcaattgc
     1921 tcagaaagaa tttcctgatg cgctctctgc gtttagactt aatttctgga tttccttctt
     1981 gctccttgta ttctctttta acttcttgtt tatccatttt catatctttc ataaataaaa
     2041 aatattcagc gataaaatca aaaattaaga caattatcat actgccgaga caatacagta
     2101 tgagaaggaa tagcatctct ccccaatctg ataataaaga tatgatatct ccatcaagag
     2161 ttttaaaaag cagtgattta tgattactcc aaaatacttt gatcgccaat gcaaaaaaaa
     2221 caatataaag aattgcttta acaaattctt ttacggtttt taagccaaat atacgcttta
     2281 agccatttac tggattcaat gaatcaaact taaatttaac agcttcagtt gctaattgca
     2341 ttttactttg aaaccaggag acaagtattg ttgtgaacat gcaaaccagc aaaaaaggaa
     2401 tgttcgtttt caaccaacca acccccatag ctttaaaata gtcccaaata tctggtttag
     2461 cgttatgatc aaggatgtat tcaagaatcg acataatgtg atgcacatca aaaacatatc
     2521 cgagatataa agtacccaca agcattatta cagagatggt taagtctcta ctttttagaa
     2581 tttgcccttt tttagaagca tcctggagtt tcttttgtgt tggcttttca gttttattgg
     2641 ccatgaaaca ctctatttct caatgtatag ttgcaattgt tctgggaaaa aggataacgg
     2701 cattactcgt tcagccagta ttggggcaaa ataaataatt aatattaaaa atgccagcgc
     2761 acttttgaca gtcagtgaaa tggcaaaagc atttagttgt gaagcataac atgccagcaa
     2821 tcccaggaca gcctcaccgc ccagcataac ggcaatcacg gggcttgcgt aaacgatagt
     2881 atgagtcatt atgttattaa tatatgagaa taaaggctct aatttaggaa aattaaaatt
     2941 gcctgaaggc cataaattat aactttgcca aagagtttcc agaataaagt tcattccacc
     3001 tttagtaaga taaaccgcag cagagaaaag gttaaataat cttgctaatt ccgaggtatc
     3061 gacacctgta gccggatcca aagtactact taatgttgca ccgcgttggt tatcgataaa
     3121 gctacctata gcaaggaaca tccaaaacgg ggatgctaat aagcatgcaa taaacagccc
     3181 tatgataatt tctctggcag cgaacaaagc aatatcgatt tcatttaagt tcaataattc
     3241 atagtggtaa tgctgaacta atgcacatga taccactatt attattgggt gacgaactac
     3301 tgttggtata ttcccactgg cgaaaaatgg taacaaatag aaagttggtg ccaatcggca
     3361 aaaaccgagc gctgtagcag ccagcagact atgcaactga tagagtatcg cctcccccat
     3421 gctatcctct cgcgaatgct aaattcagca tttcgatacc aaatgaatat aatttttcac
     3481 cataccaccc gctcattaaa aaaaagcaaa tacttacgca caataatttc acgccaaatg
     3541 gcaacgtctg ttcttgcaac tgcgttaccg tttgaaaaag ccctaccaat aaaccaacaa
     3601 atgtcgcaac ttcaataggt cctgcagaca taactaaaat cagatacagc gccctattcc
     3661 ccgcaaaaac aatatcgtcc atattttttc ctcagggatt aatggatagg tcaaaatatt
     3721 gtaaaatgag cccttttgat aacattgtcc aaccgtccat cgcgacaaac aaaattaatt
     3781 ttatcggtgt agatatggtt accggactca tcatcatcat acctaacgtt aacagaacgc
     3841 tggatataac cagatccact acgacaaagg gtaaatagat ataaaaacct ataataaatg
     3901 cactttttat ctcactcaat gcatatgcgg gcagtaacga gaaaattgaa atattatcat
     3961 catcgattat ctcttcattg tcttcagaac tattcacctt ctgaatcttt tcaaaaaagc
     4021 taaccaactc aggttcagaa tattttatca aatatgattt ataaccactc atgcctgtct
     4081 caacaaaatt aacaaccgat gctacattat tgaatgaaag attctcattc tgactgttat
     4141 tatatatttc cttcccaacc ggcatcatta cgaacattga caatagcaaa gcaaccccat
     4201 taagtgtcat atttgatggc acttgctgaa gtcccagcgc gtttcggaca ataacaaaaa
     4261 caatcgagaa tttaataaaa catgtcccgg atgctattat aaacggcaat agtgtaaata
     4321 atgataatat cgctatcagc gaaatgctat ttgacattat tattccctga taaccaagaa
     4381 tgaatttcta cgcccaattt atcatccact tcaacaagtt cgccatagcc agttagcgca
     4441 ccatttacac gtatctctat atttttctca gattcaggaa gcagagatat tattcttttt
     4501 gcacagagat cgtctatttc atatagattc attattttct taccaagaac aaactcaatt
     4561 tttacaggca actcttcaaa actattaatc atctgttcgt cattttcatc ataaatctca
     4621 ctctcgtttt ttttgatatc aaaatcatct gtttcaaaat cttc
//
LOCUS       DAZFXT010000224         4582 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.224, whole genome shotgun sequence.
ACCESSION   DAZFXT010000224 DAZFXT010000000
VERSION     DAZFXT010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4582
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.224"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..1138
                     /gene="ampC"
                     /locus_tag="ACHKW0_004810"
     CDS             5..1138
                     /gene="ampC"
                     /locus_tag="ACHKW0_004810"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7477478.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1227..1760
                     /gene="blc"
                     /locus_tag="ACHKW0_004811"
     CDS             1227..1760
                     /gene="blc"
                     /locus_tag="ACHKW0_004811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7477479.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1757..2074)
                     /gene="sugE"
                     /locus_tag="ACHKW0_004812"
     CDS             complement(1757..2074)
                     /gene="sugE"
                     /locus_tag="ACHKW0_004812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7477480.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAIKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2256..2402)
                     /gene="ecnB"
                     /locus_tag="ACHKW0_004813"
     CDS             complement(2256..2402)
                     /gene="ecnB"
                     /locus_tag="ACHKW0_004813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7477481.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2513..2638)
                     /gene="ecnA"
                     /locus_tag="ACHKW0_004814"
     CDS             complement(2513..2638)
                     /gene="ecnA"
                     /locus_tag="ACHKW0_004814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7477482.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2690..3256)
                     /gene="efp"
                     /locus_tag="ACHKW0_004815"
     CDS             complement(2690..3256)
                     /gene="efp"
                     /locus_tag="ACHKW0_004815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HFO7477483.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3298..4326
                     /gene="epmB"
                     /locus_tag="ACHKW0_004816"
     CDS             3298..4326
                     /gene="epmB"
                     /locus_tag="ACHKW0_004816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0072581 - protein-N6-(L-lysyl)-L-lysine
                     modification to protein-N6-(beta-lysyl)-L-lysine [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HFO7477484.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         1185 a         1166 c         1138 g         1093 t
ORIGIN      
        1 ccttatgttc aaaacgacgc tctgcgcctt attaattacc gcctcttgct ccacatttgc
       61 tgcccctcaa caaatcaacg atattgtgca tcgcacaatt accccgctta tagagcaaca
      121 aaagatcccc ggtatggcgg tggcggtaat ttatcagggt aaaccttatt actttacctg
      181 gggctatgcg gacatcgcca aaaagcagcc cgtcacacag caaacgttgt ttgagttagg
      241 ttcggtcagc aaaacattta cgggcgtgct tggtggcgac gctattgctc gaggggaaat
      301 caagttaagc gatcccacaa caaaatactg gcctgaactt accgctaaac agtggaatgg
      361 gatcacacta ttacatctcg caacctacac tgctggcggc ctgccattgc aggtgccgga
      421 tgaggtgaaa tcctcaagcg acttgctgcg cttctatcaa aactggcagc ctgcatgggc
      481 gccaggaaca caacgtctgt atgccaactc cagtatcggt ttgttcggcg cactggctgt
      541 gaagccgtct ggtttgagtt ttgagcaggc gatgcaaaca cgtgtcttcc agccactcaa
      601 actcaaccat acgtggatta atgtaccgcc cgcagaagaa aagaattacg cctggggata
      661 tcgcgaaggc aaggcagttc atgtttcgcc aggggcgtta gatgctgaaa cttatggtgt
      721 gaagtcgacc attgaagata tggcctgctg ggtacgaagc aatatgaatc cccgtgatat
      781 caacgacaaa acacttcagc aagggataca actggcacaa tctcgctact ggcaaaccgg
      841 cgatatgtat cagggcctgg gctgggaaat gctggactgg ccggtaaatc ctgacagcat
      901 cattaacggc agtggcaata aaattgcact ggcagcacac cccgtaaaag cgattacgcc
      961 cccaactcct gcagtacgcg catcatgggt acataaaaca ggggcaaccg gcggatttgg
     1021 tagctatgtc gcgtttattc cagaaaaaga gctgggtata gtgatgctgg ctaacaaaaa
     1081 ctatcccaat ccagcgagag tcgccgccgc ctggcagatt ctcaacgctc tacagtaaaa
     1141 tttcatcggg tccgaatttt cggacctttt ctgcgctttt ccttgctgtc atctacactt
     1201 agaaaaaaac cagtaaggaa acatttatgc gcctgctccc tctcgttgcc gcagcgacag
     1261 ctgcatttct ggtcgttgcc tgcagttctc ctacgccgcc gcgtggcgta acagtagtga
     1321 ataatttcga cgccaaacgt tatctcggta cctggtatga gattgcccgt tttgatcacc
     1381 gttttgaacg tggactggaa aaagtcaccg caacatacag cctgcgtgat gacggcggcc
     1441 tgaatgtcat taataaaggc tataaccctg acagaggaat gtggcagcag agtgaaggga
     1501 aagcgtactt taccggcgca ccaactcgcg ctgcgctgaa agtgtcattc tttggtcctt
     1561 tctatggcgg ttataacgtt attgcactcg atcgggaata ccgccatgcg ctggtttgcg
     1621 gcccggaccg cgactacctg tggatactct cccgcacgcc aaccatttct gacgaagtga
     1681 aacaggagat gctggcagtc gcgacccggg aagggtttga tgtcagtaag tttatttggg
     1741 tacagcagcc cggtagttag tgagtgctga gtttcagacc aataatcccc aatacgatta
     1801 acgccagact cgccaggcgc atcgggttag cggactcacc gagcagcaca atgccggtga
     1861 tagccgcgcc gactgcgcca atacccgtcc acacggcata agccgtccct actggtaacg
     1921 atttaatcgc ccaggcaagt agcgccatac tgacaatcat cgccgtcacg gtaataacac
     1981 tcggcgtcaa acgactaaag ccgtgggtat atttcaggcc aacggcccat accacttcca
     2041 gcagaccagc aataactaag ataatccagg acatatcagg ctccggagaa ttggggccgt
     2101 ccccggtgaa aagaggcgtt tgcaggtcgt cctgcaaagc tgatggttga acggatattt
     2161 tgccaggtgg caggggaatt tcaactatta ccttagtgac tatcgcagaa aaaacaaacg
     2221 gcacgacata cctgtcgtac cgttattgct tattgctatt gctgcgcttt cgttgctgcg
     2281 ccagaaatcg cgttaccgcc atcagaaatg tcttcaccaa cgccacgcgt ggtgttgcag
     2341 gcagttaata ctgttgaaag caccagaaca gaaaagatcg ctgcaattgt cttcttcacc
     2401 ataacgtctt ccttttaggt aatgttattt atgtttgcct atagcaaatt aataatagac
     2461 aacatcgcca aaaatgacgg aaatctgatg atttttcgga agagaagaaa aattagctgg
     2521 cagcgcgaga gatggagttg ccgagatgtt tgatgtcttc gccgaaaccg cgagcggtgt
     2581 tacagcccgt gagcagcgtg ctggcaagca aaacaagaac gataaggcgt ttcatcatct
     2641 ctgacctgaa taagtgatgg tgcagcctgc aggccgcacc acaaccgcat tacttcacgc
     2701 gagagacgta ttcaccagag cgggtatcca ctttgatgac ttcgccgatt tgtacgaaca
     2761 gcggaacctt aaccacagcg ccagtagaca gggtagccgg tttgccgcca gtacctgcgg
     2821 tatcaccttt caggcccgga tcggtatcaa cgatttccag ttcaacgaag ttcggcggag
     2881 taacggagat cggctgacca ttccacagag ttacgataca ctctgcctga tccagcagcc
     2941 atttagcgtt gtcgccaatt gcttttgcat cagcagacag ctgctcgaaa gtttcgttgt
     3001 tcatgaagtg ccagaactca ccgtcgttgt acaggtaagt caggttcata tcgacaacat
     3061 cagcgccttc agcggaatca gtagatttga aggttttttc tacgcgagta ccggtcagca
     3121 gacgacgcag tttaacgcga gcaaatgcct ggcctttacc cggttttacg aattcactcg
     3181 cttcaaccgc gtaaggttcg ccgtctaaca tgattttaag accagcacga aaatcgttgc
     3241 tatagtacgt tgccataagg ccctctgaaa tttgttaatt ggtagctaag ccacaaaatg
     3301 gcgcatattg taaccctaaa taccccatcc agagaagatt ggttaacgca acttgccgat
     3361 gttgtgaccg atcctgatga acttctgcgt cttttgaata tagacgcgga cgaaaaactg
     3421 ttggccggac gcagcgccaa aaagctgttt gccctgcgtg tgccccgctc atttatcgat
     3481 cgcatggaga aaggcaatcc ggacgatcct cttttgcgtc aggtacttac ctcgcaagat
     3541 gagtttgtcg tcgcgtccgg attctccacc gacccgctgg aagaacagca cagcgtagtg
     3601 cctggtttgt tgcataaata ccacaaccgg gcgcttttgc tggtcaaagg cggctgcgcg
     3661 gtaaattgcc gctattgctt ccgtcgccac ttcccctatg ccgaaaatca gggcaacaag
     3721 cgtaactggc agactgctct tgagtatgtt gctgcgcatc cggaactgga cgagatgatt
     3781 ttctccggcg gcgatccgct gatggcgaaa gatcacgagc tggactggtt gctcacacaa
     3841 ctggaagcca tcccgcatat caaacgtctg cggattcaca gccgtctgcc gattgtgatc
     3901 ccggcacgta tcaccgaggc gctggttgaa cgctttgccc gttctacgct gcaaatcttg
     3961 ctggtgaatc acatcaatca tgccaatgag gtagatgaaa cgttccgtca ggcgatggct
     4021 aaattgcgcc gtgtcggtgt caccctgctt aaccagagcg ttctgttacg tggtgtgaac
     4081 gataacgcac aaacgctggc aaacctgagt aatgcgttgt tcgatgccgg cgtaatgccg
     4141 tattacctgc atgtgctcga taaagtacag ggcgcggcgc attttatggt gagtgatgac
     4201 gaagcacggc agattatgcg tgagttgctg acactggtgt cgggttatct ggtgccgaaa
     4261 ctggcgcgag aaatcggcgg cgaacccagc aaaacgccgc tggatctcca gctacgccag
     4321 cagtaactga ttaaacaggg aagttaaaca ttagcctgat gcgctacgct tatcaggcct
     4381 acattgtgca ttgaaattga atggatttgc aatgaactgt aggccggata aggcgtttac
     4441 gccgcatccg gcaagagcaa caaacgcaag tacagcaacc tgtcattaaa ctgggaaatt
     4501 taatctttgc acgttgcata acctttaatt attcatatgc attgctgttg ttacttttta
     4561 tatagctaac accaataatt aa
//
LOCUS       DAZFXT010000225         4461 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.225, whole genome shotgun sequence.
ACCESSION   DAZFXT010000225 DAZFXT010000000
VERSION     DAZFXT010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.225"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..114
                     /locus_tag="ACHKW0_004817"
     CDS             <1..114
                     /locus_tag="ACHKW0_004817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HFO7477485.1"
                     /translation="TLLGERIMDRMTMNGGRWVTFNWDSWRPNVSNQRVVK"
     gene            136..852
                     /locus_tag="ACHKW0_004818"
     CDS             136..852
                     /locus_tag="ACHKW0_004818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7477486.1"
                     /translation="METVFDALKALKKASSQVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEHHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKAFPQPAGVALPVGEAE
                     TQEEIKTESVAVTVQSQPSFTRKHPDGLILPSLHVANRELRRAKGQVQKWERVCAALR
                     ELNKCRDILRDITATREQQR"
     gene            885..1166
                     /locus_tag="ACHKW0_004819"
     CDS             885..1166
                     /locus_tag="ACHKW0_004819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HFO7477487.1"
                     /translation="MILRQCAGTRKVKSVGALIGRTEAAVRTKARELGISMMLRGDFH
                     PSAKYSQRDIELARQLHQRGMQRREIARKLGMPLRIVNNYVYFDRRVSA"
     gene            1163..1390
                     /locus_tag="ACHKW0_004820"
     CDS             1163..1390
                     /locus_tag="ACHKW0_004820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477488.1"
                     /translation="MKILYQDYGPVGQVVISSTVMEFRKHNRVVDAVLLTCPGISASR
                     AGVFIMKTKLYGSKAWIKKAYRVALQEVNSE"
     gene            1383..1694
                     /locus_tag="ACHKW0_004821"
     CDS             1383..1694
                     /locus_tag="ACHKW0_004821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477489.1"
                     /translation="MSKIKEMPVVRDGYGYWTHPEYEKFCDGREYISTEEFNAWMEEN
                     NLQYVLCFRDEGCADLDACDADISAWEPERPEGDGWFIGSIHDTEDGPVCVWLRNKAE
                     A"
     gene            1822..2040
                     /locus_tag="ACHKW0_004822"
     CDS             1822..2040
                     /locus_tag="ACHKW0_004822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HFO7477490.1"
                     /translation="MKHYLEKNYPRKSRTTEFLFFILFIVLMIPISPLLLVWIIGRTF
                     EPVIELYTDVTWESFSALHNKINPYKEN"
     gene            2042..2599
                     /locus_tag="ACHKW0_004823"
     CDS             2042..2599
                     /locus_tag="ACHKW0_004823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7477491.1"
                     /translation="MSTITRERAEIKSYITGFLSDSAHDNKSSDSLLANVFRIALASL
                     EAEPIAMVVPDEMDLLTCHLDGVTKTYADGWNACRVAMLQAGNFRENKNSSTNNFREI
                     SETSTRSPITLDGWISCTERMPEKSQNVLISMNIDSEAGPLIYSARYLGGTFRRGGIA
                     VSPGNDLRQATHWMSLPEPPQEVNQ"
     gene            2890..3045
                     /gene="hokD"
                     /locus_tag="ACHKW0_004824"
     CDS             2890..3045
                     /gene="hokD"
                     /locus_tag="ACHKW0_004824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7477492.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESEK"
     gene            3165..3509
                     /locus_tag="ACHKW0_004825"
     CDS             3165..3509
                     /locus_tag="ACHKW0_004825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiW/YdeI family stress tolerance OB fold
                     protein"
                     /protein_id="HFO7477493.1"
                     /translation="MKKVLIAALISGVSFGAFAQQGGFQGPEAERSTVAQAKELKDDA
                     WVILEGSIVKKVGDERYEFRDNSGTIVTDIDDSVWAGQNVSPKDKVRIEGEIDKDLSS
                     VEVDVKALKLLK"
     gene            3631..3903
                     /locus_tag="ACHKW0_004826"
     CDS             3631..3903
                     /locus_tag="ACHKW0_004826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477494.1"
                     /translation="MTQHIKSHNSEADPEIKQGRRFRAPQYGWFHYLFCTIDEADMLQ
                     EAYLRRGVRVERSLNADRLTWTVSVYLPVRAHLPRTHACYRQRVWR"
     gene            3905..>4461
                     /locus_tag="ACHKW0_004827"
     CDS             3905..>4461
                     /locus_tag="ACHKW0_004827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7477495.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESLPVFHRGRVLVEPEPKNMRA
                     LPSGAVPAVRQPLAEDKSLLPFFSDERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHH
                     SETVIHSYGAGAMVLCWHCDNQLRDQTSESLEQLTQQNLTAWMIDVIRHVMNGTQERE
                     LSLAELSWWAVCNQVVDALPEAVSRR"
BASE COUNT         1182 a          962 c         1276 g         1041 t
ORIGIN      
        1 acgcttcttg gtgagaggat tatggaccgc atgaccatga acggtggtcg gtgggtgacg
       61 tttaactggg atagctggcg tccaaatgtc agcaatcaga gggttgtaaa gtaatttttg
      121 ttggaggacg ttttaatgga aactgtattt gacgcactga aagcactgaa aaaagcctct
      181 tcacaggtag tggcatcgcg ccttggaatc agccgcgaag atgctgtcaa cgaactgtgg
      241 aaactgaagc gccgtggtga agcggataac aagggttcga tgtggtggct gattcaggct
      301 ggtgaaagtg aaccagtgtc accggtaccg aaagtgacag cgcaaatgct gactgaggcg
      361 attgaacatc atggcccaca aacggcggat gagctggcac tgatgttcgg gattacctcc
      421 cgccgggcga attcatcact ggccatggca atcagcaaag ggcgtctgat tcgcgtgaat
      481 cagggcggta aatttcggta ctgcataccg ggcgctgatt taccggcaga gccgaaagcc
      541 gcatccatag cggaaacgga tggtaaagcc tttcctcagc cagcaggtgt tgcgttacca
      601 gtcggggaag cggaaacaca ggaagaaata aaaacggaaa gtgtggcggt cacagtgcag
      661 tcacagccgt cgttcaccag aaagcatccg gatggtctga ttttaccatc gctgcatgtg
      721 gctaaccgcg agctgcgccg ggcaaaaggt caggttcaga agtgggagcg agtctgcgcc
      781 gcgctgcggg agctgaacaa gtgccgggat attctccggg atattaccgc caccagagaa
      841 cagcagcggt gagtgggtgg aagacgtggt gccgggcgga aatcatgata ctccggcagt
      901 gtgcgggaac gaggaaggta aaaagcgttg gcgcacttat cggacgaact gaagcggcag
      961 tgagaacgaa ggcacgggag ctgggcatca gcatgatgtt acgtggtgat tttcacccgt
     1021 cggcaaaata ttctcagcgt gatattgagc tggcgcggca actgcatcag agaggcatgc
     1081 aaagaaggga aattgccaga aaattaggca tgccgctgcg catagtgaat aactacgttt
     1141 atttcgacag gagggtgtct gcgtgaaaat cctgtatcag gattacggcc cggtggggca
     1201 ggtggttatc agcagtactg taatggagtt tcggaagcat aaccgtgtgg tggatgctgt
     1261 gctgttaacc tgtccgggga tatcggcgag tcgtgcaggt gtgtttatta tgaagacgaa
     1321 attatatggc agtaaggcgt ggataaagaa ggcgtatcgt gtagcgttgc aggaggttaa
     1381 cagtgagtaa aattaaagaa atgccggtag ttcgtgacgg atatggctac tggacacatc
     1441 ctgaatatga aaaattctgt gatggtcggg aatatatttc aacggaagag tttaacgcct
     1501 ggatggagga aaataatctt caatacgtcc tctgcttcag agatgaagga tgtgctgacc
     1561 ttgatgcgtg tgatgctgat atttctgcat gggaaccgga acgaccagag ggcgatggct
     1621 ggtttattgg ttccattcat gatacggaag atggcccggt ttgtgtctgg ttgcgaaata
     1681 aggctgaagc ataaaggcga taaaccaact aacaactaaa tactgaagat ttaaatcaga
     1741 aacgattttt attaaatcct taaccggagg gattcctgca ccctcagaac atcaggaggc
     1801 cgcccgaaag ggcggtaaga aatgaaacat tatttagaaa aaaattaccc acgaaagagc
     1861 agaacaacag agtttctgtt tttcattctg tttatagtgt tgatgatacc gatatccccg
     1921 ctattactgg tctggataat tggaaggaca tttgaaccag ttattgagct atataccgat
     1981 gtgacatggg aatcattcag cgcactgcac aataaaatta atccgtataa ggaaaactga
     2041 tatgagcact attaccagag aacgcgcgga gattaaatca tacatcacag gcttcctgag
     2101 cgactcggcg cacgataaca agtcttcaga cagcctgctg gctaatgtgt ttcgtatcgc
     2161 gctggcatca ctggaagcag agccgatagc aatggtagtg cctgatgaaa tggatttgct
     2221 tacctgccat ctcgacggtg taactaaaac atatgctgat ggctggaacg cctgccgcgt
     2281 cgccatgctt caggccggaa actttcggga aaataagaat tcgtcaacca acaattttcg
     2341 ggaaatctcg gaaacgtcaa ccagatctcc gataactctg gatggctgga taagctgtac
     2401 tgagcgaatg cctgaaaaga gccagaacgt gcttatttcg atgaatatcg atagcgaggc
     2461 tgggccatta atatattccg cacgctatct cggaggcacg ttccggcgcg gaggtatagc
     2521 agttagtccg ggtaatgatc ttaggcaagc aacccactgg atgtcgctac cagaaccgcc
     2581 gcaggaggtg aatcaatgac ctggcctgaa gcattcacaa cggtaggaat tgcgatggcg
     2641 gtggcgctgg tggtgtattc gatttgccgc tggggataaa aacggtttgc gggaaaagga
     2701 tagttaagta gaattgcagc gggtgcttga ggctatctgc ctcgggcatg aacaccaacg
     2761 gcagatagag aaaagcccca gttaacatta cgcgtcctgc aagacgctta acattaatct
     2821 gaggctcaat ccatgctgaa cacatgtagg ttagcctctt acgtgccgaa aggcaaggag
     2881 aagcaggcta tgaagcagca aaaggcgatg ttaatcgccc tgatcgtcat ctgtttaacc
     2941 gtcatagtga cggcactggt aacgaggaaa gacctctgcg aggtgcgaat ccgaaccggt
     3001 cagacggagg tcgctgtctt cgtagactac gaatctgaga agtaagagac caggcggggg
     3061 agtaatctcc cgccacctct gatgtgtcag gcatcctcaa cgcacccgcg ctttaccata
     3121 ctgaaaatgc tgtttgaatg ttcatctctg aaagaggact atgaatgaaa aaggtattga
     3181 ttgcagcact tatttccggt gtgtcttttg gcgcttttgc acagcagggt ggtttccagg
     3241 ggccagaagc agagcgttca acagtagcgc aggcaaaaga actgaaggat gatgcatggg
     3301 ttatccttga agggagcatc gttaaaaaag tgggtgatga acgttatgag tttcgtgaca
     3361 atagcgggac aattgtcacg gatattgatg acagcgtatg ggccgggcag aatgtttctc
     3421 cgaaagacaa agtaagaatt gagggtgaaa ttgataaaga cctgagcagt gttgaagttg
     3481 atgtaaaggc actgaaatta ttaaagtaac cgcccctgct tgttaagccc gtcttactga
     3541 cgggttttct gtttgtacat tccggcgtat tgccttacaa ttcgcgcagt cagcctgaac
     3601 aactgacacc tgctgtcacc ggagaatccg atgacacaac acataaaatc ccacaattct
     3661 gaagccgacc cggaaattaa gcaggggagg cgttttcgtg cgcctcagta tggctggttt
     3721 cactatctgt tctgtacgat cgatgaggca gatatgcttc aagaggcgta tctgcgtcgc
     3781 ggtgtccgtg tggagcggag tctgaacgct gatcgtctga cctggaccgt ttctgtatat
     3841 cttcctgttc gtgcacatct gccacggaca catgcctgct accgtcagcg cgtctggagg
     3901 taatgtgcgg gtattacttc gacctgttct ggttccggaa ctcgggctgg tggtccttaa
     3961 gcccggtcgt gaatcattgc cagtttttca tcgcggcagg gtgctggtgg agccggaacc
     4021 gaaaaacatg cgggcgctgc catctggagc ggttcctgct gttcgccagc cgctggcgga
     4081 agataaatca ctgctgccat ttttcagcga tgagcgggtg attcgtgcag ctggcggcgc
     4141 tggtgcactg tctgactggt tattacgtca cgtgaaatcc tgccagtggc cacacggcga
     4201 ttatcatcac agcgaaaccg ttattcacag ttacggtgct ggcgcaatgg tgttgtgctg
     4261 gcactgcgac aaccagctgc gcgaccagac ctccgaatca cttgagcaac ttactcaaca
     4321 aaatctgaca gcctggatga ttgacgtcat acgccatgta atgaatggca cgcaggagcg
     4381 ggaattatcg ctggctgaat tatcctggtg ggcagtctgc aatcaggtgg tggacgcatt
     4441 acctgaggca gtatcgcgtc g
//
LOCUS       DAZFXT010000226         4422 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.226, whole genome shotgun sequence.
ACCESSION   DAZFXT010000226 DAZFXT010000000
VERSION     DAZFXT010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.226"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..318
                     /locus_tag="ACHKW0_004828"
     CDS             <1..318
                     /locus_tag="ACHKW0_004828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfO"
                     /protein_id="HFO7477496.1"
                     /translation="AAECDGEVVRPKEPNWTAWANEIRLMCVQDGRNHKQICEMYSRV
                     SRDPFWCRNVLSPSKLREKWDELSLRLSPSISTYTEKREDPYFKSSYDNVDYSQIPAG
                     FRG"
     gene            315..809
                     /locus_tag="ACHKW0_004829"
     CDS             315..809
                     /locus_tag="ACHKW0_004829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7477497.1"
                     /translation="MSMSLLNEVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHYCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYVGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARREQCLQKSSKRAASGEEWYLSGN
                     YVGA"
     gene            809..1462
                     /locus_tag="ACHKW0_004830"
     CDS             809..1462
                     /locus_tag="ACHKW0_004830"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009007 - site-specific
                     DNA-methyltransferase (adenine-specific) activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HFO7477498.1"
                     /translation="MSNKYCQALVELRNKPAHELKEVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPAWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMVA"
     gene            1459..1785
                     /locus_tag="ACHKW0_004831"
     CDS             1459..1785
                     /locus_tag="ACHKW0_004831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003878599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HFO7477499.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRSLLVGEENARLRAAHWLH
                     ERGLKV"
     gene            1782..2171
                     /locus_tag="ACHKW0_004832"
     CDS             1782..2171
                     /locus_tag="ACHKW0_004832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7477500.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDSQVKRML
                     VEWGPVIPEGKVEITISKYEKPAGAAA"
     gene            2191..2988
                     /locus_tag="ACHKW0_004833"
     CDS             2191..2988
                     /locus_tag="ACHKW0_004833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005634 - nucleus [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HFO7477501.1"
                     /translation="MNNLMVIDGIEVRRDAYGRYSLNDLHRAAGSLDKHKPAFWLRNE
                     QTERLISELQICNSVNMAPVNVIRGGNNQGTYVCKELVYAYAMWISPSFHLKVIRTFD
                     MVTSTPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNLAPRY
                     AIDAPADAPDGSSRPTLSLSALLKQYGIRLTANQAYHQMAKLGIVEQRERYSRTAINN
                     IKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            2996..3985
                     /locus_tag="ACHKW0_004834"
     CDS             2996..3985
                     /locus_tag="ACHKW0_004834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7477502.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAATQPLADDPAVRTVFRNEAVIRRAGGVECHESWLLREKGCQWPHSDWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLVRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            4003..4359
                     /locus_tag="ACHKW0_004835"
     CDS             4003..4359
                     /locus_tag="ACHKW0_004835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7477503.1"
                     /translation="MRDMYEVMDRWGAWAASDNSGVDWQPIAAGFKGLLPHGKKSRIQ
                     CDDDEGIMIDSCVARLRRYKPEEYELIIAHFVIGISLRTIAKKRKCSDGTIRKELQTA
                     MGFVDGCLAMLAYSMA"
BASE COUNT         1070 a         1049 c         1298 g         1005 t
ORIGIN      
        1 gccgccgaat gtgacggcga ggtggttcgt cccaaagaac cgaactggac agcctgggca
       61 aacgaaattc gcctgatgtg tgtgcaggat ggtcgtaatc ataaacaaat ctgcgagatg
      121 tacagccgcg tcagtcgcga tccgttctgg tgccgtaacg tgctcagccc gtcgaagctg
      181 cgggaaaaat gggatgagct ttccctgcgc ttatcgccgt ccatcagcac atacacagaa
      241 aaacgcgaag acccatattt caaatccagt tacgacaacg tggactacag ccagatcccg
      301 gcaggattca gggggtgagc atgagtcttt tgaatgaagt tcagaaattc attgaagccc
      361 atccgggctg tacttccgga gacattgcgg atgcttttgc aggttactca cggcagcgcg
      421 ttctgcagtc agcaagcaag ttacgtcaga gtgggcgtgt ggctcactat tgtgaagggg
      481 atacacgcag acatttcccg cgcctgactg agagagcgca ggagccggaa ccgcaaccag
      541 ttcgtgaaac cagacctgtg cgcaatttct atgtcggcac taacgatcca cgggtgattt
      601 tgtgcctgac ccgccaggct gaagaactgg agtccagggg cctataccgt cgtgctgcaa
      661 cggtgtggat ggcggcattc cgtgaaagcc actcccagcc agaacgaaac aattttctgg
      721 cgcgtcgtga gcagtgctta cagaaaagca gcaagcgcgc tgcatcgggt gaagagtggt
      781 atctgtcagg gaattacgtg ggggcttaat gagtaataaa tattgccagg cgctggtgga
      841 actgcggaac aaaccagccc atgaactgaa ggaagtgggc gatcagtggc gcacgccgga
      901 taacattttc tggggaatta acaccctgtt tggcccgttt gttctggatc tgttcactga
      961 cggtgataac gccaaatgtg ccgcgtatta cacggcggaa gacaacgcgc tggcgcatga
     1021 ctggtcagaa cgtcttgcgg agcttaaagg tgctgccttt ggtaatcccc catacagccg
     1081 cgccagtcag catgaggggc aatacatcac aggcatgcgt tacatcatga agcatgccag
     1141 tgccatgcgt gataaaggtg ggcgctatgt tttcctgatc aaagctgcca ccagcgaagt
     1201 gtggtggccg gaagatgcag atcatattgc ttttattcgc gggcgtatcg gttttgaact
     1261 gcctgcctgg tttatcccga aggatgagaa gcaggtgccg acaggcgctt tcttcgctgg
     1321 tgctattgct gttttcgaca agacctggaa gggaccggca atcagctaca tcgggcgcga
     1381 tgaacttgag gcatgtggtg aggcctttct ggcgcaggtt cgccagcagg cagaaaaact
     1441 ggtcagggag atggtggcat gacgacgtta actcaatgcc agcagcaggt gctggatatg
     1501 ctgatttctt atcagaaaga acgtggcttc ccgccaacca atcaggaggt ggcaaccatg
     1561 ctgggatacc gttcagtgaa tgcagcggta gaacatcttc gcgcactgga gaaaaaaggc
     1621 gtcatcacga taaagcgtgg cgtggcccgg ggcatcacgc ttcataccgc ggtgaaggac
     1681 gacgacagcg aggcggtcgg gattatccgc tcactgcttg tcggtgagga aaacgccagg
     1741 ctgcgtgcag cccactggtt acatgagagg ggcctgaaag tatgaagctg atcctgcctt
     1801 tcccgcccag cgtgaacacg tactggcgac accccaacaa aggggcgttt gcaggtaaga
     1861 gcctgataag cgcggcgggg cgcaaattcc agagcgcggc gtgtgcagca atagttgagc
     1921 agttacgtcg tctgccaaaa ccaacgtcgg cacctgcttc agtggagatc gtgttgtttc
     1981 ctccggataa ccggatccgc gatctggaca actataacaa ggcgctgttt gacgccctga
     2041 cccacgcggg tgtgtgggaa gacgacagtc aggtgaaaag aatgctggtg gagtggggac
     2101 cggttatccc ggaagggaag gtcgagatca ctatcagtaa gtacgagaaa ccggcgggtg
     2161 cagccgcctg attaagagga gaaacgaagt atgaataatc tgatggttat tgatggtatt
     2221 gaagttcgtc gtgatgctta tgggcgttac agcctgaacg atctgcacag ggctgccggt
     2281 tctctggata aacataagcc tgcattctgg ctccgcaatg agcaaaccga acgtttaata
     2341 agcgagttgc aaatttgcaa ctcggtcaat atggcgccag ttaacgttat tcgtggcgga
     2401 aataaccaag ggacgtatgt ctgcaaagaa ctggtgtatg cctatgcaat gtggatcagc
     2461 ccgtcattcc atctgaaggt gatccgtact ttcgatatgg taaccagcac accggaaaaa
     2521 ttatccgggc aggctgctga caagatgcag gctggcgtga tcctgctgga ctttatgcgc
     2581 cgggagttaa atctgtctaa ctcttcagtg cttggtgcct gtcagaaact ccaggaggct
     2641 gttggcttac cgaatctggc accgcgctat gccattgatg ctcctgctga cgcgcctgat
     2701 ggctcaagcc gccccacgct gtcactgagt gcactgctga agcagtatgg tatccgcctg
     2761 acagctaatc aggcatatca ccagatggcg aagctgggga tcgttgaaca acgcgaacga
     2821 tacagccgta ccgcgattaa caacatcaaa aaattctggt cgctgacggc gaaaggctgc
     2881 atgttcggca agaacatcac cagtcctgca aatccgcgcg agacgcagcc gcatttcttc
     2941 gaatcccgat tccctgagct gttaaagcta ctcgataccg ttcattgagg tgaccgtgag
     3001 agcactactg acccctgaaa ttgccccgcg tatggggatc gtattgttca ggccaggttc
     3061 agagctgatg cccctgttta tgcaggggcg tgtcctgctg gagccagagc cggaacgtta
     3121 ttcatctttc gccagtggtg ccgttccggc ggcaacacaa ccgctggcgg atgatcctgc
     3181 cgttcggacc gtgttccgca atgaggcagt gatccgtcgt gctggtggcg tggaatgtca
     3241 tgaaagctgg ttacttcgtg aaaagggctg tcagtggcct cattctgact ggcacagcga
     3301 gaacatgacc acaatgcggc acgctccggg agcaattcgt ctgtgctggc actgcgataa
     3361 ccagctgcgt gaccagttca cggaacggct ggaatcaatg gcaacggata actgtgcccg
     3421 ctgggtgttg tctgtcgtgc gtcgggatct cggttttgat gacagtcacg ttgtgacaat
     3481 gccggaactg tgctggtggc tggttcgtaa tgatctggcg gatgccttac cggaaagtgc
     3541 agcccgtaag gcactgagat taccgaagcc tgttgtgcca tctgtcaccc gggaaagtga
     3601 ccttgtgcct tcggttcctg ccaccagcat catccaggat aaggcgaaaa aggtgctggc
     3661 gctgaaagtg gatccggagt cgccggagtc ttttatgtta cgcccaaaac gtcgccgctg
     3721 ggttaatgaa aagtacacgc gctgggttaa gacgcagccg tgtgcatgtt gtggaaagcc
     3781 tgctgatgat ccccaccatc tgataggcca cggtcagggg ggaatgggta caaaagcgca
     3841 tgatctcttt gtgttgcctt tgtgcagaaa gcatcacgac gagctgcatg cggataccgt
     3901 ggcatttgaa gagaagtatg gctcccagct ggagttgata tttcgtttta tcgatcgtgc
     3961 gctggcaatt ggtgtgctgg cctgattttg tggagaaagt tgatgcgtga tatgtatgaa
     4021 gtaatggatc gttggggagc ttgggctgct tcagacaata gcggagtgga ctggcagccg
     4081 atagcggctg gtttcaaggg acttttacct catggcaaaa agtcacggat tcagtgtgat
     4141 gatgacgaag gcatcatgat agacagttgt gtggctcggt tgagaaggta taaaccagag
     4201 gaatatgagc tcatcatcgc ccactttgtt atcggtatct cattacgcac tattgcgaag
     4261 aagagaaaat gctctgatgg cacaattagg aaggaactgc aaactgcaat ggggtttgtt
     4321 gacggctgtt tagcaatgtt agcttatagt atggcataaa aaataaaata gatttactgc
     4381 cgatttttca aaaaagactg ggaactgttt atatccaacg ta
//
LOCUS       DAZFXT010000227         4390 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.227, whole genome shotgun sequence.
ACCESSION   DAZFXT010000227 DAZFXT010000000
VERSION     DAZFXT010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.227"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..209)
                     /locus_tag="ACHKW0_004836"
     CDS             complement(<1..209)
                     /locus_tag="ACHKW0_004836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7477504.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="ACHKW0_004837"
     tRNA            complement(324..399)
                     /locus_tag="ACHKW0_004837"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="ACHKW0_004838"
     tRNA            complement(406..480)
                     /locus_tag="ACHKW0_004838"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="ACHKW0_004839"
     tRNA            complement(597..681)
                     /locus_tag="ACHKW0_004839"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="ACHKW0_004840"
     tRNA            complement(690..765)
                     /locus_tag="ACHKW0_004840"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKW0_004841"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="ACHKW0_004841"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HFO7477505.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKW0_004842"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="ACHKW0_004842"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HFO7477506.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKW0_004843"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="ACHKW0_004843"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HFO7477507.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1123 a         1103 c          984 g         1180 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt
//
LOCUS       DAZFXT010000228         4361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.228, whole genome shotgun sequence.
ACCESSION   DAZFXT010000228 DAZFXT010000000
VERSION     DAZFXT010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.228"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..2805
                     /locus_tag="ACHKW0_004844"
     CDS             <1..2805
                     /locus_tag="ACHKW0_004844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HFO7477508.1"
                     /translation="LASENPDEAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDS
                     QPGTLNDFLGAMTEDDARPEALRRFEQMVEEVARNASVVAQNTAAAKKSASDAGTSAR
                     EAATHATDAADSARAASTSAGQAASSAQSASSSAGTASTKATEAEKSAAAAESSKSAA
                     ATSAAAAKTSETNAAASQQSAATSASTATTKASEAATSARDASASKEAAKSSETNASS
                     SASSAASSATAAANSAKAAKTSETNARSSETAAGQSASAAAGSKTAAASSASAASTSA
                     GQASASATAAGKSAESAASSASTATTKAGEATEQASAAARSASAAKTSETNAKASETS
                     AESSKTAAASSASSAASSASSASASKDEATRQASAAKGSATTASTKATEAAGSATAAA
                     QSKSTAESAATRAETAAKRAEDIASAVALEDASTTKKGIVQLSSATNSTSESLAATPK
                     AVKAAYELANGKYTAQDATTAQKGIVQLSSATNSTSEMLAATPKSVKAAYDLANGKYT
                     AQDATTAQKGIVQLSSATNSASETLAATPKAVKAANDNANGRVPSARKVNGKALSADI
                     TLTPKDIGTLNSTTMSFSGGAGWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGI
                     SELVLRAGNGNPKGITGALWQRTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWD
                     YTSNASVTIHTSPAYSANKPEGLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYAL
                     MQGQTFDKSAYPKLATAYPSGVIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASA
                     SSTDLGTKTTSSFDYGTKSTNNTGAHTHSVSGTAASAGNHTHSVTGASAVSQWSQNGS
                     VHKVVSAASVNTSAAGAHTHSVSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNA
                     AGNAENTVKNIAFNYIVRLA"
     gene            2805..3380
                     /locus_tag="ACHKW0_004845"
     CDS             2805..3380
                     /locus_tag="ACHKW0_004845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0098004 - virus tail fiber assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7477509.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSID
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     GGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPALP"
     gene            complement(3478..4068)
                     /locus_tag="ACHKW0_004846"
     CDS             complement(3478..4068)
                     /locus_tag="ACHKW0_004846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HFO7477510.1"
                     /translation="MSRIYAYCRVSTLEQTTKNQRREIESAGFNIKSQQIIEEQISGS
                     AATSERPGFNQLLDRLKRGDALIVTKLDRLGCNPMDISKTVEQLTKAGIKVHCLALGG
                     VDLTSPPGKMMMQVISAVAEFERDLLLERTHSGILRARGAGKRFGRPPVLNDEQIQRV
                     LEQIKSGINISDIAQEFKTSRQTILRAKAKNQTPDI"
BASE COUNT         1166 a         1067 c         1154 g          974 t
ORIGIN      
        1 ctggcctcag aaaatccgga tgaagccggg cgttacagca tggacgttga gtacgggcag
       61 tacagcgtta ttctgttggt ggaaggattc ccgccgtcac atgccgggac catcaccgtg
      121 tatgaagatt ctcaacccgg tacgctgaat gattttctcg gtgccatgac ggaggatgat
      181 gcccgtccgg aggcactgcg ccgttttgaa cagatggtgg aagaggtggc gcgtaacgcg
      241 tccgtggtgg cacagaacac ggcagccgcg aagaagtcag ccagtgatgc cggcacatct
      301 gcccgtgagg cggcaaccca tgcgactgat gctgcagatt cagcacgtgc agccagcacg
      361 tcagccggac aggccgcttc gtcggctcag tcagcgtctt ccagcgcagg aacggcatca
      421 acaaaggcca ctgaagcgga aaaaagtgct gccgctgcag agtcctcaaa aagcgcggca
      481 gctaccagtg ccgctgccgc gaaaacgtca gaaacgaatg ctgcagcgtc acaacaatca
      541 gccgccactt ctgcatccac cgcgaccacg aaagcgtcag aagctgccac ctcagcccgg
      601 gatgcgtcgg cttcaaaaga ggcggcaaaa tcatcagaaa cgaacgcatc ctcgagcgcc
      661 agtagcgcag cttcctcggc aacggcggca gcaaattccg cgaaggcggc aaaaacgtcc
      721 gagacgaacg ccaggtcttc tgagacggca gcgggacaga gcgcctcagc tgcggcaggc
      781 tcaaaaacag cggctgcgtc gtctgccagt gccgcgtcaa caagtgccgg gcaggcctca
      841 gccagtgcca ccgctgccgg aaaatcggca gaaagcgccg catcgtccgc ttcaacagcc
      901 acaacgaagg ctggcgaagc cactgaacag gccagcgcag cagcgaggtc tgcttccgca
      961 gcgaagacat ccgagacgaa cgcgaaagcg tcggaaacca gcgcagaatc ctcaaaaacg
     1021 gctgccgcat cgtccgccag ttcggcggcg tcatcggcat catctgcgtc tgcttcaaaa
     1081 gatgaggcga ccagacaggc gtcagcagca aagggcagcg ccacgacggc atccacgaag
     1141 gcgacagagg cagctggcag tgcgacggcg gcagctcaga gcaaaagtac ggcggaatcc
     1201 gcggcaacgc gcgccgagac agcggcaaaa cgggcagagg atattgcatc cgccgtggcg
     1261 cttgaggatg cgagcacgac gaaaaagggg atagtacagc tcagcagtgc gactaacagc
     1321 acttccgagt cactggcggc aacgccaaaa gccgttaagg ccgcgtatga gctggctaac
     1381 gggaaataca ccgcacagga tgcaacgaca gcacagaaag ggatagttca gctcagcagc
     1441 gcgaccaaca gcacatctga aatgctggcg gcaacgccaa agtcggtaaa ggcagcctat
     1501 gaccttgcta acgggaaata tactgctcag gacgctacga cagcacaaaa aggaattgtc
     1561 cagctcagta gtgcaaccaa cagcgcatct gaaacgcttg ccgcgacacc gaaagcagtg
     1621 aaagcagcta atgataatgc gaatggtcgg gtaccttctg cccgtaaggt gaatggtaag
     1681 gcgctttcag cggatataac actgacgccg aaagatattg gtacgcttaa ctcaacaaca
     1741 atgtcattca gcggtggtgc tggttggttc aaattagcaa cggtaaccat gccacaggcg
     1801 agttctgttg tttcaattac gttgattggt ggcgcgggat ttaacgtggg gtcacctcaa
     1861 caggcaggta tatctgaact tgttttgcgt gcaggtaatg gtaatccgaa ggggattact
     1921 ggtgctttat ggcagcgcac atcgacaggg tttacaaatt ttgcctgggt caatacatct
     1981 ggtgatactt acgatattta cgttgcaatc ggaaattatg cgactggtgt aaatattcaa
     2041 tgggattata ccagtaatgc cagcgtgact attcatacgt caccagcata ttctgctaat
     2101 aagccggaag ggttaacgga cggtacagtt tattcactct atacaccatc agagcagttt
     2161 tatccgcctg gcgcaccaat cccgtggcca tcagataccg ttccgtctgg ttatgccctg
     2221 atgcaggggc agacttttga caaatccgct tacccgaaac ttgcaaccgc ttatccgtca
     2281 ggcgtgatcc ctgatatgcg tggctggacg attaagggca aacctgccag tggtcgggcc
     2341 gtattgtctc aggaacagga cggcattaaa tcgcacaccc acagcgccag tgcatccagt
     2401 acggatttgg ggacgaaaac cacatcgtcg tttgattacg gcactaaatc cacgaataac
     2461 accggggcgc atacgcacag tgtgagcggt acagccgcaa gtgccggaaa ccatactcat
     2521 agtgtcacag gcgcatcagc agtcagccag tggtcacaaa atgggtcagt acataaggta
     2581 gtgtctgcgg ccagtgtgaa tacaagtgct gcaggagcgc acactcatag tgtcagcggc
     2641 acagctgcat ctgcaggtgc tcacgcacat actgtcggta ttggtgctca tacgcactct
     2701 gttgcgattg gctcacatgg acacaccatc accgttaacg ctgctggtaa cgcggaaaac
     2761 actgtcaaaa acatcgcatt taactacatt gtgaggcttg cataatggca ttcagaatga
     2821 gtgaacaacc acggaccata aaaatttata atctgctggc aggaactaat gaatttattg
     2881 gtgaaggtga cgcatatatt ccgcctcata caggtctgcc agcaaacagt atcgatattg
     2941 caccgccaga tattccggca ggcttcgtgg ctgttttcaa cagtgatgag gcatcgtggc
     3001 atctcgttga agatcatcgg ggtaaaacgg tttatgacgt ggcttccggc gacgcgttat
     3061 ttatttctga actcggtccg ttaccggaaa atgttacctg gttatcgccg ggaggggaat
     3121 atcagaagtg gaacggcaca gcctgggtga aggatacgga agcagaaaaa ctgttccgga
     3181 tccgggaggc ggaagaaaca aaaaacagcc tgatgcaggt agccagtgag catattgcgc
     3241 cgcttcagga tgctgtggat ctggagatcg caacggagga agaaaccttg ttgctggaag
     3301 cttggaaaaa gtatcgggtg ttgctgaacc gtgttgatac atcaactgca ccggatattg
     3361 aatggccagc cctaccgtag ggtaagatat ataaattcta taattagaag tatctttcca
     3421 tttaaggcta ggaagggggg cttggaaaac gtaaggaatc tcacaccgag attattttta
     3481 tatatcaggt gtctgatttt ttgctttagc tcttaaaatg gtttgccgcg aggttttgaa
     3541 ttcttgggca atgtcactta tatttatacc tgacttaatt tgctctagta ccctctgtat
     3601 ttgttcatca tttaacacag gtgggcgacc aaatcgcttt cctgcgcccc gtgctcttaa
     3661 tatccctgaa tgagtacgct caagtaaaag gtctcgctca aattcagcga ctgctgaaat
     3721 tacttgcatc atcatttttc caggtggact ggtcaggtca acgcccccca atgctaagca
     3781 atgcactttg atacctgctt tggtcagttg ttccaccgtt ttactgatat ccattggatt
     3841 acaaccgagg cgatccagtt ttgtgacaat caatgcatca cctcttttca gtcgatcaag
     3901 caactggtta aaaccaggac gctcactggt tgctgctgag ccgctaattt gttcttcaat
     3961 tatttgctga gatttgatgt taaaacctgc actttcgatt tcccgacgtt gatttttggt
     4021 tgtctgttcc agagttgata cccgacagta agcataaatt cgagacatag tgagatcttc
     4081 tatacgaaat tggtgtacat atcataatgc atctcagaaa ataattttga ttatttttgt
     4141 acatatttgt atgtacacgt tcgaaaataa acgaatgcgt atgcaacccc gtaattttgg
     4201 tgagacccaa aatcgatttt gtgaaaaatg gccttaactc ggtttgtttt tcgagttccg
     4261 ggcggactca aggaagaaga atagtgttgc gtgttatttt aaccagattt caagttgttt
     4321 ggtcgtggaa aagtggagca aaatgttgtt aaagtggaaa a
//
LOCUS       DAZFXT010000229         4283 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.229, whole genome shotgun sequence.
ACCESSION   DAZFXT010000229 DAZFXT010000000
VERSION     DAZFXT010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.229"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            236..1168
                     /gene="rfaD"
                     /locus_tag="ACHKW0_004847"
     CDS             236..1168
                     /gene="rfaD"
                     /locus_tag="ACHKW0_004847"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008712 - ADP-glyceromanno-heptose
                     6-epimerase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HFO7477511.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1178..2224
                     /gene="rfaF"
                     /locus_tag="ACHKW0_004848"
     CDS             1178..2224
                     /gene="rfaF"
                     /locus_tag="ACHKW0_004848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HFO7477512.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2228..3220
                     /gene="rfaC"
                     /locus_tag="ACHKW0_004849"
     CDS             2228..3220
                     /gene="rfaC"
                     /locus_tag="ACHKW0_004849"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HFO7477513.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3217..>4283
                     /gene="rfaL"
                     /locus_tag="ACHKW0_004850"
     CDS             3217..>4283
                     /gene="rfaL"
                     /locus_tag="ACHKW0_004850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HFO7477514.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKN"
BASE COUNT         1131 a          974 c         1047 g         1131 t
ORIGIN      
        1 tatccattca aatgcttccg ttatttatca tacatcggaa tattgatact aaagcactat
       61 tcacatgcaa aaccaacatc cgccatgaag gactagctaa aacccaaact agtttgttgc
      121 aattagcatc cttgcacctc tatgtaaagg gctgaaggga ttcggatgtg atggtatgat
      181 tacagacatt cgtgtctgag attgtctctg actccataat tcgaaggtta cagttatgat
      241 catcgttacc ggcggcgcgg gctttatcgg cagcaacatc gttaaagccc tgaatgataa
      301 aggcatcacc gatattctgg tggtggacaa cctgaaagac ggcaccaagt ttgtgaacct
      361 ggtggatctg aatatcgcag actatatgga taaggaagac ttcctgatcc agattatggc
      421 tggcgaagag ttcggcgatg tcgaagcgat tttccacgaa ggcgcgtgct cttccaccac
      481 cgagtgggac ggcaagtata tgatggataa caactatcaa tactccaagg agctgctgca
      541 ctactgcctg gagcgtgaaa tcccgttcct gtacgcttct tccgcagcca cctacggcgg
      601 acgcacctcc gactttattg aatcccgcga gtacgaaaaa ccgttgaacg tctacggtta
      661 ctcaaaattc ctgtttgatg aatatgttcg ccaaatcctg ccggaagcga actcgcagat
      721 tgttggcttc cgctatttca acgtttatgg accgcgtgaa ggccataaag gcagcatggc
      781 gagcgtcgct ttccatctca acacccagct taataacggt gaatcaccga agctgttcga
      841 aggtagcgag aactttaaac gcgacttcgt ctatgttggc gatgtggcag atgtaaacct
      901 gtggttcctg gaaaatggcg tttccggcat cttcaatctc ggcaccggtc gtgcggaatc
      961 cttccaggct gtagctgatg ctacgctggc ttatcacaag aaaggccaga tcgaatacat
     1021 tccgttcccg gataagctga aaggccgcta ccaggcgttc actcaggcag atctgacaaa
     1081 tctgcgcgcg gcgggttacg acaaaccgtt caaaaccgtt gctgaaggtg taacggaata
     1141 catggcctgg ctgaatcgcg acgcataaga gctctgcatg aaaatactgg tgatcggccc
     1201 gtcttgggtt ggcgacatga tgatgtcgca aagtctctat cgcacgctcc aggcgcgcta
     1261 tccccaggcg ataatcgatg tgatggcacc ggcatggtgc cgtccattat tatcgcggat
     1321 gccggaagtt aacgaagcta tccctatgcc tctcggtcac ggagcgctgg aaatcggcga
     1381 acgccgcaaa ctgggtcata gcctgcgtga aaagcgctac gaccgcgcct acgtcttacc
     1441 taactccttc aaatccgcat tagtgccttt cttcgcgggt attcctcatc gcaccggctg
     1501 gcgcggcgag atgcgctacg gtttactcaa cgatgtacgc gtgctcgata aagaagcctg
     1561 gccgctaatg gtggaacgct atatagcgct ggcctatgac aaaggcatta tgcgcacagc
     1621 acaagatctg ccgcagccat tgttatggcc gcagttgcag gtgagcgaag gtgaaaaatc
     1681 atatacctgt aatcaatttt cgctttcatc agaacgtccg atgattggtt tttgcccggg
     1741 tgcggagttt ggtccggcaa aacgctggcc acactaccac tatgcggagc tggcaaagca
     1801 gctgattgat gaaggttatc aggtggttct gtttggctcg gcgaaagatc atgaagcagg
     1861 caatgagatt cttgccgctt taaataccga gcagcaggca tggtgtcgga acctggcggg
     1921 ggaaacacag cttgatcaag cggttatcct gattgcagcc tgtaaagcca ttgtcactaa
     1981 cgattctggc ctgatgcacg ttgcggcggc gctcaatcgt ccgctggttg ccctgtatgg
     2041 tccaagtagc ccggacttca caccgccgct atcccataaa gcgcgcgtga tccgtctgat
     2101 taccggctat cacaaagtgc gtaaaggtga cgctgcggag ggttatcacc agagcttgat
     2161 cgacattact ccccagcgcg tactggaaga actcaacgcg ctattgttac aagaggaagc
     2221 ctgacggatg cgggttttga tcgttaaaac atcgtcgatg ggcgatgttc tccatacgtt
     2281 gcccgcactc actgatgccc agcaggcaat cccagggatt aagtttgact gggtggtgga
     2341 agaagggttc gcacagattc cttcctggca cgctgccgtt gagcgagtta ttcctgtggc
     2401 aatacgtcgc tggcgtaaag cctggttctc ggcccccata aaagctgaac gcaaagcgtt
     2461 tcgtgaagcg ctacaagcag agaactatga cgcagttatc gacgctcagg ggctggtaaa
     2521 aagcgcggca ctggtgacac gtctggcgca tggcgtaaag catggattgg actggcaaac
     2581 cgctcgcgaa cctttagcca gcctgtttta caattgtaag catcatattg caaaacagca
     2641 gcacgccgta gaacgcaccc gcgaactgtt tgccaaaagt ttgggctata gcaaaccgca
     2701 aacccagggc gattatgcta tcgcacagca ttttctgacg aacctgccta cagatgctgg
     2761 cgaatatgcc gtatttcttc atgcgacgac ccgtgatgat aaacactggc cggaagaaca
     2821 ctggcgagaa ttgattggtt tactggctga ttcaggaata cggattaaac ttccgtgggg
     2881 cgcgccgcat gaggaagaac gggcgaaacg actggcggaa ggatttgctt atgttgaagt
     2941 attgccgaag atgagtctgg aaggcgttgc ccgcgtgctg gccggggcta aatttgtagt
     3001 gtcggtggat acggggttaa gccatttaac ggcggcactg gatagaccca atatcacggt
     3061 ttatggacca accgatccgg gattaattgg tgggtatggg aagaatcaag tagagtgtcg
     3121 ttctacaagt atgtctcttg cagatttgcc agctcaaacc gtttttcaaa atttaaatct
     3181 tgaaataata accaataagt tgacatcgga gataagatga cctcaacatt atttttctct
     3241 ctcgagaaaa aaaactggat agcgtactgg aacagagctc tcgtattctt attcattacc
     3301 acctattttt tgggtgggat tacaaggtat aaacatctta ttgttattct tatgacaata
     3361 acgacaatcg tctatctctg caaacggcca aaacactatc tctcactatt taaaacattt
     3421 ctttttggta gtgttgccat attaactatt gctgcattgc tgtcacttct tcaatcccct
     3481 gatgcaggtg ctagcatgaa ggaaattttc aaagctatta ttgagaatac tttactatgc
     3541 acaatagcaa taccgatcat attgagagac gagaaaagag aagatgtcga aaaaatcgtt
     3601 ttcttctcat ttattagtgc gttgggctta cgctgttttt ctgaattgat tacctattat
     3661 aaggactatc aacaagggat aatgccattc gcagattata gacaccgtag catttctgac
     3721 tcgatggtct ttttattccc tgcattgtta aatctctggc ttatcaaatc agcaaaatac
     3781 cgcatttctt ttgtggttct aagcgttatt tttatttttc tgatattagg aactttatcc
     3841 agaggggcct ggctttccgt gttagtcatt ggattaatat ggattctgat gtttaaacaa
     3901 tggaagttac tattagtagg agtaatggtt gccatcattg cattgtcggt tattttcaca
     3961 cataaggaga tgaccgcaaa gctaacgtat aaacttcaac aaactaatag ttcttatcgc
     4021 tatgcaaatg gtactcaagg cagcgcactc gatctaatat tagaaaatcc tgttattggt
     4081 tatggttacg gtaacgttgc atataaagat gtctataata aacgtgtcat tgattatcca
     4141 gaatggacct ttagacaatc aatagggcca cataattttg cgctattcat ctggtttggc
     4201 actggtttat tagggctggt aagtcttatg atgctatact gtgcaatatt gaaagagtgt
     4261 ataaaaaatg gcgtcaagaa taa
//
LOCUS       DAZFXT010000230         4272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.230, whole genome shotgun sequence.
ACCESSION   DAZFXT010000230 DAZFXT010000000
VERSION     DAZFXT010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.230"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..426
                     /locus_tag="ACHKW0_004851"
     CDS             <1..426
                     /locus_tag="ACHKW0_004851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7477515.1"
                     /translation="NHTTAYRDGAGIWTICRGATRVDGKPVIPGMKLSKGKCDQVNAI
                     ERDKALAWVEKNIKVPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEA
                     IRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
     gene            700..1395
                     /locus_tag="ACHKW0_004852"
     CDS             700..1395
                     /locus_tag="ACHKW0_004852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor protein"
                     /protein_id="HFO7477516.1"
                     /translation="MKEMISVDHEISMSSLDFLNNIINPARAEAGEVPHEPRKFLAKI
                     EDELELDGTGKKFRLNNNQTRTAYYDLDFDQMMLVGMRESKAVRRSVLARLKAMHGIQ
                     IPRTLPEALRFAAKLAEQKAVLENQLAIAAPKAEFVDNYVEASGLMGFREVAKLLGIK
                     ETDFRLFLLENGIMYRLAGKMTPYSHHLDAGRFSVKTGEAGNGHAFTQVKFTPKGVQW
                     IAGLLAAWRATAA"
     gene            1490..1621
                     /locus_tag="ACHKW0_004853"
     CDS             1490..1621
                     /locus_tag="ACHKW0_004853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477517.1"
                     /translation="MSDKLIMLAKGLCVIVGISFSLMLVALFLSITWMALTSAGLVG"
     gene            1844..2029
                     /locus_tag="ACHKW0_004854"
     CDS             1844..2029
                     /locus_tag="ACHKW0_004854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7477518.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPEWIMQPA
                     PDWQTPLNGIISPSESG"
     gene            2268..2819
                     /locus_tag="ACHKW0_004855"
     CDS             2268..2819
                     /locus_tag="ACHKW0_004855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7477519.1"
                     /translation="MNYPTIVNGIDFRDLVFVTGNEPVTDTRKVAVAFGKEHKDVLRK
                     TRAVVQQCSKEFAERNFALCYENNELQNGKPQPIYRMTKDGWTMLVFGFTGKAAMTFK
                     EAYIQAFNWMAELIQQGLANLEAERNAVMLEYMKEKDVASMSGRLLNRWGRVKKPQLL
                     ARLDRLEQQGQIALPGFDKGISA"
     gene            2827..3042
                     /locus_tag="ACHKW0_004856"
     CDS             2827..3042
                     /locus_tag="ACHKW0_004856"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477520.1"
                     /translation="MRRIVAVSSPYPRINRDRSPTGRLLYESVRDNQKRYTPGFTALT
                     ERGVVPHGRWSGAMVEETGRCVTSQAL"
     gene            3165..3491
                     /locus_tag="ACHKW0_004857"
     CDS             3165..3491
                     /locus_tag="ACHKW0_004857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HFO7477521.1"
                     /translation="MKRNLPLIILLSSLVMGCTQHKTDMPRQLVKALPQYPAYAAANY
                     IKGRVDVKFDIGADGTVTRIEFIRSEPHHLFDEQVVKAMAKWRFEKDRPCKGVKKTFI
                     FSPSAP"
     gene            complement(3623..3823)
                     /locus_tag="ACHKW0_004858"
     CDS             complement(3623..3823)
                     /locus_tag="ACHKW0_004858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7477522.1"
                     /translation="MSSKNRTRRTTTRNIRFPNHIIEQINIALEHKGSGNFSAWVIEA
                     CRRRLATDATHLRPASMTNNEK"
     gene            3865..4230
                     /locus_tag="ACHKW0_004859"
     CDS             3865..4230
                     /locus_tag="ACHKW0_004859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7477523.1"
                     /translation="MAELRTLQSRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT         1078 a          927 c         1190 g         1077 t
ORIGIN      
        1 aaccacacca cggcataccg tgatggtgcg ggtatctgga ccatctgccg tggagccacc
       61 cgggtggatg gtaagcctgt tattcctggc atgaagctgt cgaaggggaa atgcgaccag
      121 gttaacgcca ttgagcgtga taaggcgctg gcatgggtgg agaaaaacat caaagtgcca
      181 ctgactgaac cccagaaagc gggtattgcg tcattctgtc cttacaacat tggcccaggt
      241 aagtgtttcc cgtcgacgtt ttataaacga attaatgcag gagatcgaaa aggtgcctgc
      301 gaagcgattc gctggtggat taaggacggt ggcagagact gccgtattcg ctcaaataac
      361 tgttatggtc aggtatcccg tcgtgaccag gagagcgcgc tggcgtgctg gggaatcgac
      421 agataagcag aatattttgc tgaaaaatga cgttggccag cgcgggcgga taacacgaaa
      481 tcctgcgaac tggcaaaatg taagtgaata aagttaggca gattatttca cgcagaggca
      541 ccgtaatggt gcctttgtca tttctgcgct tcgcacaagc gtaaataaac caaagaacct
      601 ttcaggatga gccctggtgg ataaccggca gtggtctggt taaccctctt tgggctggtt
      661 attcctgtgc gcagggttca tcactaaaag gaatcaacca tgaaagagat gatttctgtc
      721 gatcatgaaa tatccatgag tagtctggat tttctgaata acattattaa tccagcccgg
      781 gcagaagccg gagaagtccc tcatgaaccg cgtaagtttc ttgcaaaaat tgaggatgag
      841 ctagagcttg atggaaccgg aaaaaaattc cggttaaaca ataaccagac aagaacggca
      901 tactatgatc tggattttga ccagatgatg ctcgttggca tgagggagtc aaaggccgtt
      961 cgtcgttctg tgctggcaag actcaaagcg atgcatggta ttcagatccc ccggacttta
     1021 cctgaggcgt tgcgatttgc ggcaaaactg gctgaacaga aagcagtgct ggaaaatcaa
     1081 ctggcaatag cagcgccgaa ggctgaattt gttgataact atgttgaagc atctggtctg
     1141 atgggattcc gggaagttgc taagttactc ggtatcaaag aaaccgattt ccggctgttt
     1201 ttgttggaga acggaataat gtatcgcctc gctggaaaaa tgacgcctta ctcgcatcac
     1261 ctggatgcgg ggcggtttag cgtgaaaacg ggcgaggcgg gcaatggtca tgcttttacg
     1321 caggttaaat tcaccccaaa aggtgttcag tggattgctg gtctgcttgc tgcatggaga
     1381 gctaccgcag catgaagatg ataaaaatgg actggaaatt tttgctggtc tggctgattc
     1441 cgtttttatg ggtggttgcc cggttaatta ctgctattaa gaggtaaaga tgtcagacaa
     1501 actcataatg ctggcgaagg gcctttgtgt aatcgtcggt atttcatttt cactaatgct
     1561 ggttgctctt tttctttcca taacctggat ggcgttgact tcggcagggc tggtggggtg
     1621 agcataaacc gaatgctttc cgcgtttacc gttattctgc tggtggtctg tagtgcgctg
     1681 tggctggcaa cagaccatta ccgtgataac gccatcacct acaaagcgca gcgcgataaa
     1741 aaagccagag agctggagct ggcaaacgca accattactg acatgcagca gcgccagcgt
     1801 gatgttgctg cgcttgatgc cagatactca agggaattag ccgatgcgag agctgaaaat
     1861 gaaactctgc gtgctgatgt tgccgctggt cgtaagcgcc tgcggatcaa cgccacctgc
     1921 tccggtaccg tgcgtgaagc caccggcacc tccggaatgg ataatgcaac cggcccccga
     1981 ctggcagaca ccgctgaacg ggattatttc accctcagag agcggttgat gacaatgcag
     2041 aagcaactgg aaggggcgca ggaatatatc cgcactcagt gcctgaatta acagagccag
     2101 cttaatcgct ggctttttca tatctgaatt tcatcgcgca tctcacgcgc atatcacatc
     2161 cccgagcctt tcagaaagtt gagcctgaga actgtcgtat atggtggcga ccatctcggg
     2221 gcggcttttc tgtgagacag gctcaatttt ctgaaaggta agacgctatg aactatccga
     2281 cgattgttaa cggtattgat ttccgcgatc ttgtttttgt gacaggcaac gagccggtga
     2341 cggatacccg aaaggttgcc gtagcatttg gcaaagaaca caaagatgta ttgcggaaaa
     2401 caagagccgt tgttcaacaa tgctcaaaag aatttgcaga gcgcaatttt gcgctttgct
     2461 atgaaaacaa tgagttacag aacggtaaac cccagcccat atatcgaatg acaaaagacg
     2521 gttggacgat gttggtattt ggttttacgg ggaaagctgc tatgaccttt aaggaagcgt
     2581 acattcaggc tttcaactgg atggctgagc ttattcagca aggtctggcg aatctggagg
     2641 cggagcgcaa cgctgtaatg ctggagtaca tgaaagagaa agatgtcgcc agtatgtcag
     2701 gtcgtttgct caatcgctgg ggcagggtga agaagcctca gttgctggca aggctggaca
     2761 ggctggagca gcaggggcag attgcgttac ccggatttga taaaggcatt tcagcctgac
     2821 atagccatgc gccgtatcgt cgccgtatcg tcgccgtatc cccgcattaa cagagaccgc
     2881 agcccgacag ggagactcct ctacgagagt gtgcgggata atcaaaaacg atacacaccg
     2941 gggtttaccg cgttaacgga gcgcggcgtt gtccctcatg gtcgctggtc cggtgcgatg
     3001 gtggaagaaa ccggacgatg tgttacctcg caagctctgt gatgtcatgt gtctgatttg
     3061 tgatttaagt cggataattg tcgttgccat taagcagagg attgatgacc gacagggtgg
     3121 cattgttaga ataagactta ttcttatctg tgccgggaat gaaaatgaaa agaaatcttc
     3181 cgttaattat tttgttgtct tctctggtta tgggctgtac gcaacataaa acagatatgc
     3241 cccgacagtt ggttaaggca ttaccacaat atccggccta tgcagcggca aattatataa
     3301 agggacgggt tgatgtgaag tttgatattg gtgctgatgg tactgtcacc cgaattgagt
     3361 ttatccgttc agagccgcac catttgtttg atgagcaggt tgtaaaggcg atggcaaaat
     3421 ggcgatttga gaaggacagg ccgtgtaaag gcgtgaagaa aacgtttatc tttagtcctt
     3481 ctgcaccctg attatttcat cagaaattaa ttatcactct gttgttattc tgtacatccc
     3541 ggcagggtaa gtcttgttcc gtcggatatg aagatgaaat attgttggag gacagtgggt
     3601 acctgctcct gtaaccgaac gttcatttct cgttatttgt catgctggcc gggcgcagat
     3661 gcgttgcatc tgttgccagc cttctcctgc aggcttcaat aacccacgct gaaaagttac
     3721 cggacccttt atgctcaagg gctatgttga tttgttcaat tatgtgattg gggaaacgga
     3781 tattgcgggt tgtggttctg cgggtccggt ttttcgatga cattttcttt cctctggtga
     3841 caagttatat gacggggatt ttacatggct gagcttcgta cactccagag cagaatcaaa
     3901 acactgaata cccggcgggt gaatattctg aagggggaac agcgtcgtgt cagtggcagt
     3961 gcccgggttt ccctcaagcg tcatatctgg ctgagggatg ccgggcagtg ctgtctctgt
     4021 ggtcgtgtgg ttgacctctg tgacagtgaa ctcgatcacc gcattgcact tcagttcggt
     4081 ggtggtaatg aggagacgaa tctctggacg ctctgcaccg aatgccatcg acaaaagtct
     4141 gctcgtgaag cggcgggtgg tatgccggac ccgacgctgc cggaggtgtc cggaggtcat
     4201 ggcagggcag acgatatcat cggactgtga cccgccccgg gggggatcat ccggcgaaaa
     4261 aaacgatcgc tc
//
LOCUS       DAZFXT010000231         4208 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.231, whole genome shotgun sequence.
ACCESSION   DAZFXT010000231 DAZFXT010000000
VERSION     DAZFXT010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4208)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4208)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4208
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.231"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(472..624)
                     /locus_tag="ACHKW0_004860"
     CDS             complement(472..624)
                     /locus_tag="ACHKW0_004860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7477524.1"
                     /translation="MDTIELGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTEAAAHRW
                     LARNAN"
     gene            complement(814..1221)
                     /locus_tag="ACHKW0_004861"
     CDS             complement(814..1221)
                     /locus_tag="ACHKW0_004861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7477525.1"
                     /translation="MNEKTLGQRIRERRKQVGLSQNDLSKAAGVSGSSISLWESDHTA
                     PRGQNLHRLAEVLQCSPTWILFGDEDKTPDPPVALNSALDLSEDELEMLRLYRALPKS
                     EQQAQISELRARVENFNRLFTELLEARKRNKHQ"
     gene            1299..1526
                     /locus_tag="ACHKW0_004862"
     CDS             1299..1526
                     /locus_tag="ACHKW0_004862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7477526.1"
                     /translation="MFKSDVINFYGTKAKVAKAAGVDPSAVSQWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRAGRLNNENHP"
     gene            1510..2061
                     /locus_tag="ACHKW0_004863"
     CDS             1510..2061
                     /locus_tag="ACHKW0_004863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7477527.1"
                     /translation="MKITPEQVCEALDAWVCRPGMTQEQATILITEAFWALKERPNID
                     VQRVTFNDGEVDQRALGVNRVKIFERWKAIDTRDKREKFTALIPAIMEAIRISDFRLY
                     REISDGKSITYMIAGLNKEYGDVVESGLLFADPAVVERETDELIEKAIAFKHAYRQQY
                     QYYFADKQISARGSYEYRCTTMG"
     gene            2033..3073
                     /locus_tag="ACHKW0_004864"
     CDS             2033..3073
                     /locus_tag="ACHKW0_004864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding domain-containing
                     protein"
                     /protein_id="HFO7477528.1"
                     /translation="MSIDALRWAKKVKTGSSSSKSVLTWLADMCGADLCAYPSVSALA
                     DVTELNKKTVQDSLRHLMEIGLIVDTGERKGRTKQIVVYRLIGVEESVAEPEYTQKRV
                     SLKVGKIGAVNKNSTENGYVSAQKSPKNGTLCCMENNQRHPNFPSKTPKNGSRNPKEP
                     KDLNPTHNARESAPTSEQEVLSLQAAPHVFLDGLSEPIGKFPMTDSWYPSRDFRRRAA
                     LWGMALPETEFTPAELTAFRDYWAAEGKVFTQIQWEQKFARHVNHVRAQVKPVSKGVN
                     HAAAPGGTASRAVQEIRAAREQWERENGFISDGNGLEAVGTHGGGLFEPLDPEERGRT
                     FEALDCTDWRDD"
     gene            3066..3527
                     /locus_tag="ACHKW0_004865"
     CDS             3066..3527
                     /locus_tag="ACHKW0_004865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein P"
                     /protein_id="HFO7477529.1"
                     /translation="MTEQQIRQVCRQCMDRCRAGETWPPDLAEFVALISESGANPFGL
                     TVDAVMEEYRRWRNESWRYNGSDKYPWPQPVLYHICLEMRSKGIERQMTEGELKRLAE
                     RQLTKWAKHVSNGLSVPPVRRQLAAPKRPLGPTPIELLKQEYERRKAAGFV"
     gene            3561..>4208
                     /locus_tag="ACHKW0_004866"
     CDS             3561..>4208
                     /locus_tag="ACHKW0_004866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7477530.1"
                     /translation="METVLHALKAMGKANSVELAARIDISREEVLNELWELKKNGVVD
                     KTGHTWFLAVEGESRVTEERPVKSETQDMLTEEVAPKVSANMMIEFIAQEGAKTCEEI
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGRFRYCMPGDNLPAEPKAALVTENDGKA
                     FPQPAGAALPVREAATQEEIKTESVAVTVQSQPLFTRKHPDGLILPSLHVANRELR"
BASE COUNT         1139 a          922 c         1151 g          996 t
ORIGIN      
        1 atgaattttg tgatgcagtg cctggtgcct ccaggtgacg ttaaccagtt aacaattaac
       61 gccggataca gagaatccac ccataacact gtttttggtt ttaactgttc cgcgtgcgct
      121 cagccgcatt caccgcatca caaaattcac tttaaaaagg gcggcagagc agtcacggag
      181 taaaactgat accgccaaac gtcaccagaa aattgataac agagggcgtt gcagcggggt
      241 tgtcacttaa gcgtatggtc agcctgacaa cccggtgtcc tcaaggggaa ggaataaccc
      301 cgccatactt accgccgcgc catttcgcgg attgccacaa ccagaagcgc acggtcgaag
      361 aaatttaacg acaagactta tatgcaaagg gatctcgccg tgcgctttcg tgttatgccc
      421 tgacttttca gggtaaatta acctgtggaa acctgttttt actggcgcta atcagttagc
      481 gtttctggct aaccagcgat gcgcggcagc ttcggtttta aacgttttac ttttggtata
      541 cgtcatcgcg gtaaacgtgc cgtcctgatt gggaaacacg ccacatacca gagattcgtt
      601 gttgccaagc tcgatagtat ccatgctgac ctcatttccc cttaacgccg ggtagcggaa
      661 ctgtttgctg agaacaccgt gcggtgtctt gatgggtggt aatttagttt tctcatgaat
      721 gttggtcaag tatttttaat gagaaaactc aatatttaat gcaaaataaa gccaatacat
      781 tgaaatgtaa ggctttaaaa tttgtgaagg gggttactga tgtttgttac gtttgcgagc
      841 ttctagtagc tcggtgaata ggcgattaaa attctcaacg cgggcacgga gttcgctgat
      901 ttgtgcttgc tgctctgatt ttggaagtgc gcgatacaat cgcaacatct ccaactcatc
      961 ttccgataag tctaaggcgc tgttgagtgc aactggtgga tctggtgttt tatcctcgtc
     1021 accaaacagt atccaagttg gtgaacattg caatacctca gccaggcgat gcaaattttg
     1081 cccgcgcggg gctgtatggt cgctttccca tagtgaaatt gatgagccag atacgccagc
     1141 ggctttgctt aaatcgtttt gacttaaacc aacctgtttg cgtctttctc taattcgttg
     1201 acctaaagtt ttctcgttca tatttagata tcttaataac ccttgacttg agattccttg
     1261 agtgattact attgagaaaa ctcaactttg gaggggtgat gtttaaatca gacgtaatta
     1321 atttttatgg gacgaaagcc aaagtagcga aagctgctgg tgttgatcca tctgctgttt
     1381 ctcaatgggg ggaactggtt cctgaaggtc gcgcgatgcg cctgcaagag gcatccggcg
     1441 gggaacttca gtacgacccc aaagtttatg acgaatatcg taaggcaaag cgggcggggc
     1501 ggttgaacaa tgaaaatcac ccctgaacag gtttgtgagg ctctggatgc ctgggtatgt
     1561 cgaccaggaa tgacacagga gcaggcgacg atattaatca cggaagcatt ctgggctctg
     1621 aaagaacgcc cgaacatcga tgttcaacgc gtcacgttta atgatggcga ggttgatcaa
     1681 cgggcgctgg gcgttaaccg ggtgaagata ttcgaacgct ggaaagctat cgacaccagg
     1741 gataagcggg aaaaattcac ggcgctgatt ccggcaatta tggaggctat ccgaattagt
     1801 gatttcaggt tgtatcgtga gatcagtgat ggaaaaagca ttacgtacat gatcgccgga
     1861 ttaaacaaag aatatggcga tgtggtggag tccgggctgc tttttgcgga tccagctgtt
     1921 gtggaacgtg agactgacga gcttatagaa aaagctattg ctttcaagca tgcgtatcgt
     1981 cagcaatatc aatattactt tgcagataaa caaatatctg ccaggggttc gtatgagtat
     2041 cgatgcacta cgatgggcta aaaaggtgaa aaccggcagt tcatccagta agtctgtatt
     2101 gacctggctt gctgatatgt gcggtgccga tttgtgtgca tacccgtctg tatctgcact
     2161 ggcagatgta acggaactga acaaaaagac tgtgcaggac agcttacgac acctgatgga
     2221 gattgggtta attgttgata ccggtgagag aaaaggcaga acaaagcaaa ttgtggtgta
     2281 ccgacttatc ggtgtagaag aaagtgttgc cgagcctgaa tacacccaaa aacgggtgtc
     2341 tttaaaggtg ggtaaaattg gtgctgttaa taaaaacagt accgaaaacg gttatgtttc
     2401 agcacaaaag agccccaaaa acggaactct ttgttgcatg gaaaataacc aaagacaccc
     2461 aaattttcca tcaaagacac ccaaaaacgg atcacggaac ccaaaggaac ccaaagatct
     2521 aaaccccaca cataacgcac gcgagagtgc tccgaccagt gagcaggaag ttttgtcgtt
     2581 acaggcagcc ccccatgtat tcctggatgg cctgagcgaa cccatcggaa aatttccgat
     2641 gaccgatagc tggtatccgt cacgggattt tcgacgacgg gctgcgttgt gggggatggc
     2701 tttgccggag acagaattta cacctgctga acttaccgca ttccgggact actgggcagc
     2761 ggaggggaaa gtgtttacgc agattcagtg ggagcagaaa ttcgcccgtc acgtaaatca
     2821 cgtcagggcg caggttaaac cagtcagcaa gggggtaaac catgcagcag caccaggtgg
     2881 caccgcatca cgggcagttc aggaaattcg ggcagcacgt gagcagtggg aacgtgaaaa
     2941 cggatttatc agcgacggaa acggcctgga agctgtggga actcatgggg gaggtttatt
     3001 cgaaccgctg gacccagaag aacggggccg caccttcgaa gctctggatt gcacagattg
     3061 gcgcgatgac tgagcagcaa atccggcagg tctgccgcca gtgcatggac cgctgccggg
     3121 cgggtgaaac atggcctccg gacctggctg agtttgtggc gctgatttcg gaaagcgggg
     3181 ccaatccatt tggcctgacg gtggatgctg tgatggagga gtatcgccgc tggcgtaatg
     3241 agtcctggcg atataacgga agcgacaaat atccgtggcc tcagcctgtg ctgtatcaca
     3301 tttgcctcga gatgcgttca aaggggattg aacgccagat gaccgaaggg gaattaaaac
     3361 ggcttgcaga acggcagctg acgaaatggg caaagcatgt tagtaacggc ctgagcgttc
     3421 cgccagtacg gcgacaactg gcggcaccaa aacgcccgtt gggaccaacg ccaattgagt
     3481 tgctgaaaca ggagtatgaa cgccggaaag cggctggttt tgtctgattt gagaagtatt
     3541 tttatccgga ggaagtttta atggaaaccg tattgcatgc actgaaagcg atgggtaaag
     3601 ccaattctgt tgaactggcg gcacgaattg atatcagccg tgaagaagtt ctcaacgaac
     3661 tgtgggagct caaaaaaaat ggcgttgttg ataaaacggg tcacacctgg tttctggctg
     3721 tcgaaggtga atcccgggta accgaagagc ggccagtaaa atctgaaaca caggatatgc
     3781 tgaccgaaga ggtcgctcca aaagttagcg ctaacatgat gattgagttt atcgctcagg
     3841 agggggctaa aacctgtgaa gaaatagcgg gtaagttcgg tgtcagtact cgcaaggttg
     3901 cttccacgct ggcggtggta accgcaacgg ggcggctggc acgcgttaat cagaacggta
     3961 gatttcgtta ctgcatgccg ggcgataatt taccagcaga gccgaaagcc gcgctggtaa
     4021 cggaaaatga tggtaaggcc tttcctcagc cagcaggtgc tgcgttacca gtccgggaag
     4081 ccgcaacaca ggaagaaata aaaactgaaa gtgtggcggt cacagtgcag tcacagccgt
     4141 tgttcaccag aaaacatccg gatggtctga ttttaccatc gctgcatgtg gctaaccgcg
     4201 agctgcgc
//
LOCUS       DAZFXT010000232         4127 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.232, whole genome shotgun sequence.
ACCESSION   DAZFXT010000232 DAZFXT010000000
VERSION     DAZFXT010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4127
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.232"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..538
                     /locus_tag="ACHKW0_004867"
                     /pseudo
     CDS             1..538
                     /locus_tag="ACHKW0_004867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731742.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
     gene            715..942
                     /locus_tag="ACHKW0_004868"
                     /pseudo
     CDS             715..942
                     /locus_tag="ACHKW0_004868"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
     gene            complement(1469..2839)
                     /locus_tag="ACHKW0_004869"
                     /pseudo
     CDS             complement(1469..2839)
                     /locus_tag="ACHKW0_004869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2859..3206)
                     /gene="tnpB"
                     /locus_tag="ACHKW0_004870"
     CDS             complement(2859..3206)
                     /gene="tnpB"
                     /locus_tag="ACHKW0_004870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7477531.1"
                     /translation="MITLPTGTRIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRDRSGKMVKILWADRDGLCLFAKRLERGRFVWPVTREGKVHLTPAQLSMLLEGIAW
                     QHPKRTERPGIRI"
     gene            complement(3203..3877)
                     /gene="tnpA"
                     /locus_tag="ACHKW0_004871"
     CDS             complement(3203..3877)
                     /gene="tnpA"
                     /locus_tag="ACHKW0_004871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element accessory protein TnpA"
                     /protein_id="HFO7477532.1"
                     /translation="MNSQTKKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFIASGIAWPLPDSVSLAQLDAILYANRKKELTEPQISEGTWRKERRASYSRE
                     FKVRLAKQALQPGAVVARIAREHGINDNLLFKWKSQYEDGLLSDDDIQECMPVPVALT
                     DTPEPTRPVTNPFWRNKPDECPESDPGNVPRCELHLKSGVVKLFDPLTPEMLRALIRE
                     MKGGTR"
BASE COUNT          929 a          993 c         1119 g         1086 t
ORIGIN      
        1 gttatgagtg gttcaagact ggctgataat catacgctat cggctggtta tgctcagagt
       61 aaggtacagg actttaagaa tattaaagga gtgaatctgc aatatcgata tgagtgggat
      121 tctccggtca gtgtcgtcgg gagtttttcg tatatgaaag gtgattgggc ggacagtcat
      181 cgcgatgaag ctgacgattt ttacagacat caggctgata ttaaatatta ttcatttctc
      241 gcaggcccag cttatcgtct gaatgactat atcagttttt atggtttggt ggggatatct
      301 catactaaag cgaagggaga ttatgagtgg agaaacagtg tgggtgctga tgagtctgat
      361 ggatatttaa gtgagtctgt atcaaaaaaa atccactgat tttgcttatg cagctggtgt
      421 tattattaat ccgtggggca atatgtctgt aaatgttggg tatgagggaa ctaaagcaga
      481 tatatatgga aaacattcgg tcaatggatt tactgttgga gttggttatc gtttttaatc
      541 attagacggg actggataat tgcacttgcg tagtgaagtt tactgaatgt ttgtatttta
      601 atgaagtatt cgacatattc ccttcagacg gaatatgtcg acggttatgg ttttactgga
      661 gcatgagtat gttatggcga aactgataaa tatatcaggt ccactgagga gagaatgacg
      721 ccaggggaga ggagggtggc ttcacgactg gagagctttc tgaatgatga ttgctttgtt
      781 tggtacgata tcccggtggg acggaaaaac cgacatccgg attttgtcat tattgacccg
      841 gataacgggc tggtatttct ggaggtcaag gactggactg tatccacatt acgtaaagca
      901 aatcaggagc aggttacact ggaaaccgac ggtcttctga aatcagaaat aaatccactg
      961 gtacaggttc gtcgttatgc ctgtgatacg gttaatgcct tacctgctga cccgtgctta
     1021 cggcagaatg acggtcagta taaggggcgg ctgaacctgg cctgggcgta tggtgtggtg
     1081 tttacccgca ttacccgtca gcaactgaag gcactgacag ggaataatga gaatgcggtg
     1141 gaaaagattt ctccttcagc acagacaatc tgtcaggatg aaatgacgca gtctgtttta
     1201 cctgaggtat tccggcaaaa aattgccggg atgtttacca cggggttcag aacccgggta
     1261 acaccccgga tgagggatat tctgcgcgcc catctgtttc ctgaagtgac cgtgaaacag
     1321 aactcacaaa tcaaaattat ggatattcag caggagattc tggccagaaa tattggtgac
     1381 gggcatcgtg tcattcatgg tgtggccggt tccggaaaaa ccatgattct tttgttccgt
     1441 tgcctttacc tggcagaaac cacgtccggt agttacgccg ccccagtgca actacccgga
     1501 gggcgttttc acacaggtta ttgtcgatct ccacccagcc attgcggcag tattcgttca
     1561 gagcgtccca ttgcttcagc agataggtga acgctttcgc tgtatccgag tggcgcgaca
     1621 gcgtgctcat ctgcccctgg agccactcat acaacgactg cattagcggt accgttctgg
     1681 cttttctgac cgccagtcgc tcttctgccg gactgccgcg gatctcagcc tcgatagcgt
     1741 acagttcccc gatacgacgg agcgcttctc ccgttactgt cgttggatgg cggacatgta
     1801 catcgtggat cttgcgtcgg gcatgtgcca tacaagccgc ttcggttacc tgaccgcttt
     1861 cgtaaagagc attgtaaccc gcatatgcat cggcctgcag gatacctctg tagtccgcca
     1921 gatgttgctg tgggtggatg cctttgcggt cgggagagta tgagaaccac accgctgccg
     1981 gcatggtcga accggcgttg cgatcgtcac gaacatagac ccacagccgt ccggtacggg
     2041 ttttaccctg acccggctcc agtacattta ccggcgtgtc atcggcatgc actttacccg
     2101 gcatcagcac atagtgcttc agttcatcat acagcgggcg aagctgctct cccatgatgt
     2161 caacccagcg ccccatcgta ttgcggtgca gctccacgcc ctggcgggca tagatttctg
     2221 actgacggta cagcggcaga tgctcggcga acttagccat gattatgcgg gccagcagag
     2281 ccggactggc gtaactgcgc tcgatgggtt ttggtggctg cggagcctga actatacagt
     2341 cgcaccggct gcaggcccgt tttgggcgaa ccgtttcgat taccctgaac gcggtgttga
     2401 tgatatccag ttgttcagag atgctttctc ccagcggttt cagtttgccg ctgcagacgg
     2461 ggcattcggt ttctgccggg gagataacct gcctgtcacg gggaagtgtt gccggaagtg
     2521 ctttgcggac gggagagtct gatgttttcg gcgctgtctc tccggccatt gaggtgagtt
     2581 gcaactgcgc ctcaccaagc atgttctgga gctcggttat acgcgcttct gcccgtgcga
     2641 tcttcttttc tatcttctcg cggcttttct cgctgctgcg accgaacaac attctctgca
     2701 gtttagcgac cagcgctctg agtgagctga tctcgcgggc atagccggtt atttcaccag
     2761 acagacggac gataacagcc tgctgtgcga tcagcagggc cttcagttgc tcgatgtcgt
     2821 cggggagtgt gttgttcatt cccctgtttt atcacgggtt atatccggat gccaggccgt
     2881 tctgtccgtt tgggatgttg ccatgcgatc ccctccagta gcatggataa ctgagctggc
     2941 gtcaggtgca ctttcccttc ccgggttacc ggccagacga agcggccccg ttccaggcgt
     3001 ttggcgaaca ggcataaccc gtcacgatcg gcccacagta ttttcaccat tttgccactg
     3061 cggtcccgga agacgaagat atgcccggag aacgggtcat ctttcagcgt gttctgcacc
     3121 ttcgaagcca ggccgttgaa gccacaacgc atatctgtga tgccagcgat gatccagatt
     3181 ctggtaccgg ttggcagcgt tatcatcggg tacctccttt catttcgcgg attagcgccc
     3241 gtaacatttc cggagtgaga gggtcaaaca gttttaccac acctgattta agatgcagct
     3301 cgcaccgtgg gacgtttccg ggatcactct cagggcactc atcaggcttg ttacgccaga
     3361 agggatttgt aactggtctg gtcggctctg gcgtatcagt cagagccacc gggacaggca
     3421 tgcattcctg tatgtcatca tcgctcagta agccgtcctc gtactggctt ttccatttaa
     3481 acagcaggtt atcattgata ccgtgctctc tggcgatccg ggcaacaaca gcaccgggct
     3541 gtaatgcctg cttagccaga cggaccttaa attcacggct gtagctggct cgccgttctt
     3601 ttcgccatgt gccttcgctg atttgaggct ctgttaattc cttctttctg ttggcataaa
     3661 ggatggcgtc aagctgagct aatgaaactg aatcgggcaa tggccatgcg ataccggatg
     3721 caataaatcg ctgaaaaagc gtatgtattg tggaatgact gagacctaga cgctgagcga
     3781 tggcccggat ggtcagttta tcttcaaatc ttaaacgcag agcatcaggc aaataagaac
     3841 ggaagcaggg aatatctttt tttgtctggg aattcatcgt tcgtgtccat ctatatagat
     3901 gggcgcgatt gttgccagac aggacaattt tcacaagacg tcgcagatgg ggcgcttaca
     3961 caacaatggc gcgagggaaa gctgctatta ccttcttcag agagccccca gctacttcct
     4021 gtgactctcg atgcagctgc cgaacagcca gaatcgctcg cagaggaccc ggaaaccctc
     4081 agtatcagct gttgtcaacg acggatgact attgcaccgt tcgtgac
//
LOCUS       DAZFXT010000233         4095 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.233, whole genome shotgun sequence.
ACCESSION   DAZFXT010000233 DAZFXT010000000
VERSION     DAZFXT010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.233"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..1723
                     /gene="dpiB"
                     /locus_tag="ACHKW0_004872"
     CDS             65..1723
                     /gene="dpiB"
                     /locus_tag="ACHKW0_004872"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HFO7477533.1"
                     /translation="MLQLHENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1692..2372
                     /gene="dpiA"
                     /locus_tag="ACHKW0_004873"
                     /gene_synonym="citB"
     CDS             1692..2372
                     /gene="dpiA"
                     /locus_tag="ACHKW0_004873"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HFO7477534.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2413..3798)
                     /gene="dcuC"
                     /locus_tag="ACHKW0_004874"
     CDS             complement(2413..3798)
                     /gene="dcuC"
                     /locus_tag="ACHKW0_004874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015556 - C4-dicarboxylate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015740 - C4-dicarboxylate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HFO7477535.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1124 a          958 c         1053 g          960 t
ORIGIN      
        1 tattttgatt caacatttta attacatccg tcaaagaggc tcgggacaac ccgcaaggaa
       61 aacaatgttg cagcttcacg agaataaaca gtttgcattt ttccaaagac tggcatttcc
      121 gctgcgtatc tttttgctga ttctggtgtt ctcaatattt gtcattgcag ccctggcgca
      181 atattttacg gccagttttg aggactattt aacgcttcat gtacgcgaca tggcaatgaa
      241 tcaggcgaaa attattgcct ccaatgacag tgtcatctct gcggtgaaaa cgcgtgacta
      301 caaacggctg gcgaccatcg ctaacaaatt acaaagagat accgattttg attatgtggt
      361 gattggggac cggcactcga tccgccttta ccatcctaat ccggagaaaa ttggttatcc
      421 tatgcagttc accaaacagg gcgcgctgga gaaaggggag agctacttca ttaccgggaa
      481 agggtcaatg gggatggcga tgcgcgccaa aacgccaatc tttgatgacg atggaaaagt
      541 catcggcgtg gtgtcgattg gctacctggt gagtaaaatc gatagctggc gggctgagtt
      601 tttattaccg atggcaggcg tgtttgtcgt gctgttaggg attctgatgt tgctgtcgtg
      661 gttcctggca gcgcatatcc gtcggcaaat gatgggcatg gagccaaagc aaatcgcacg
      721 cgtggtccgt cagcaagagg cgctgtttag ttcggtttat gaagggctga ttgcggtgga
      781 tccgcatggt tacattaccg ccatcaatcg taacgcaaga aagatgctgg ggctgagctc
      841 tcccggacgg caatggttgg gtaaacccat tgctgaagtg gtcaggcccg ccgatttctt
      901 taccgaacag attgatgaaa aacgtcagga tgtggtggct aactttaacg gtctgagcgt
      961 tattgccaat cgggaagcta ttcgttcagg tgatgatttg ctgggggcca ttatcagctt
     1021 tcgtagtaaa gacgaaattt ccaccctcaa tgcgcaactg acgcaaataa aacaatacgt
     1081 cgagagcctt cgtacattgc gacacgagca tctcaattgg atgtcgacgc tcaatggtct
     1141 gttgcagatg aaagagtatg atcgcgtgct ggcgatggtg cagggggagt ctcaggccca
     1201 gcaacagctt attgacagcc tgcgtgaggc gtttgccgat cgccaggtgg cggggctgct
     1261 ttttggtaaa gtgcagcgcg cccgggaact ggggctaaaa atgatcattg tccccggtag
     1321 ccagctttcg caactgccgc caggactgga tagcaccgag tttgcagcca ttgtgggcaa
     1381 tttacttgat aacgccttcg aagccagcct gcgtagcgat gaaggaaaca agatcgttga
     1441 attattcctc agcgatgaag gcgatgatgt ggtgattgaa gtcgccgatc agggctgcgg
     1501 cgttccagag tctctacgag acaaaatatt tgagcagggg gtcagtacgc gtgctgacga
     1561 gcccggtgaa catggcattg ggttgtactt gattgccagc tacgtaacgc gctgcggtgg
     1621 tgttatcact ctcgaagata atgatccctg cggtaccttg ttttcaatct atattccgaa
     1681 agtgaaacct aatgacagct ccattaaccc tattgatcgt tgaggacgaa acgccgctgg
     1741 cagagatgca tgcggaatat attcgtcaca ttcccggatt cagtcagata ttactggcgg
     1801 gaaacctggc gcaggcccga atgatgatcg agcgttttaa gccggggcta atcttgctcg
     1861 ataactatct tcctgacggc agagggatta atttattgca tgaactggtg caggcgcatt
     1921 atcccggcga cgtggtgttt accactgcag ccagcgatat ggaaacggtg tctgaagccg
     1981 tacgttgtgg tgtatttgat tatctcatta agcccattgc ctatgaacgg ctggggcaaa
     2041 cgctaacccg tttccgccag cgtaaacata tgctggaaag tattgatagc gccagccaga
     2101 agcaaattga tgagatgttt aatgcctatg ctcgcggtga acctaaagac gaactgccga
     2161 ccggcattga tcccttgacg ctaaacgcgg tgcgaaaact gtttaaagag cctggtgtgc
     2221 aacatacggc agaaacggtg gcgcaggcac tgaccatcag tcgcaccact gccaggcgtt
     2281 atcttgaata ttgcgccagc cgccatctga ttattgctga aattgttcac ggcaaagttg
     2341 gcagaccgca acgcatatac cacagtgggt gacatgataa agccggagga aacttgcctc
     2401 cggcattact atttacttgc ctgtgaccgc tgctgccgtt cctggcacca tcagctctgt
     2461 agcaacgata acaatcacca aaccaacaag caccggtacc gaggtgcgtt ttacgacttc
     2521 aaacggcgag atcttcgcca tcccggcaac cgcaacgact acgccagaaa cgggcgaaag
     2581 ggtacggccc aggtttgacg cctgcagcat cgggatagtc aaatacgccg ggttaatgcc
     2641 ggaagagtgc gccagtttcg ggatcatctc aacaaacgca taaaacggcg cattgcctga
     2701 accggtcgtg actgccgcca gcatggtcag aatcaccaat accagcatca ggatgatact
     2761 cgccgaacca aacgaggtag cgatagaaat cagactttga ataaagccga tggtgctaag
     2821 cccctgagcg aataccccag cggcaaccag cagcatcacc acgttagcaa acgcatctgc
     2881 cattccgcga taagccactt ccagaccaga gaaaactttc tgggtattaa agctgcggag
     2941 gaactccaga atggaggcaa tcagcataca aatcaccaga atagtgatga tgtgtaattg
     3001 cggaccccat ttaccgtcaa aaatcagtac accgatgatc ggcgtgaacg gcaaaatggc
     3061 ataaaacgca ggagcagtgg tggtgatttc actgacatct aacatttcat gagagatgtg
     3121 ctctttttta tccagataac gttgccagaa gaagtgggcg atcgccatgc cgataattgc
     3181 agcaattgag ataggcagcg tcgttttgaa ggcgaagtca atcagcgaca tttcggaagc
     3241 ttgcgccgcc agcaccacat cccctgaagt cggtgcgaga ataatcgccg ccggggaggc
     3301 acaaatggca gcagctgcgc cacgactgat accaacgttt accatcaccg gaaatagggt
     3361 tgccatcagc aaaacaccca gaccggttgc ggaagagacg gccagagaca tcagacaggc
     3421 gacaaaatag gcggcaatca tcagcaggta aggggagtta atatactgca atggttttga
     3481 cgccagcttg accaccatat cattcgcgcc gatatgggtc atgtaagcgg caaatccaca
     3541 cagcatcata atcatcatgc cgaggtcgcc gccgcggctc attagtaata ttttaacgta
     3601 ttcaacgata tccgtggcgc tgtagcctgt tgaagcctgg ctggacggta acactttgtg
     3661 ccccataatg gcactgataa tcagcaataa caggccaccg acaaataaca caccagtggc
     3721 ggaataccct ttaatgatgt agcgagctac acccacaata accacaaccc caataaggag
     3781 ttcaatgaat gtcagcataa tttttcctgt ctccaggccc caaagtaaat aataaaaaat
     3841 tcctaaagct taaggaaaaa atatgcccaa taaattggcg atgaatgctg attaaaatca
     3901 agaaaaactg ccattaagac attgaagttg ctgtttttat acatagataa caaaacgcat
     3961 atttagcggt tggcaattat cacataataa tgtgtgtaaa ttgatggtgg attgatgcaa
     4021 atttgttaat tgcaatggtg ttagctgcgg ctgtgcgctc aaaaaataat ctaacatgag
     4081 cataggttga cgatt
//
LOCUS       DAZFXT010000234         4037 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.234, whole genome shotgun sequence.
ACCESSION   DAZFXT010000234 DAZFXT010000000
VERSION     DAZFXT010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.234"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            259..1083
                     /gene="cif"
                     /locus_tag="ACHKW0_004875"
     CDS             259..1083
                     /gene="cif"
                     /locus_tag="ACHKW0_004875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000652082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector Cif"
                     /protein_id="HFO7477536.1"
                     /translation="MKDITLPPPTSASCLTGAISVNTEAVLSPMQHTSALHVRDFASL
                     CSQNLKANVLLNSDDHEVPIHQKNPAAIMQNIDSNIKQMATDWGMSIEEVEVIIGREK
                     GIVEPSCGVTANAIMKLFLDKDGFSYCFENEQTLSLEQLQERLSCMPECKSFVLRVND
                     GALGHAYIVDIPKGENSCRPAFLYQSDLGEGVTRKLRFEDWMTHKALTPILLDDICNY
                     FSCMSQNKTDLEQIATLFDIDGNVKMLRKENIQYQKHDNFSFQLFEYDTDNIEKKH"
     gene            complement(1224..1478)
                     /locus_tag="ACHKW0_004876"
                     /pseudo
     CDS             complement(1224..1478)
                     /locus_tag="ACHKW0_004876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000332260.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            1635..2210
                     /locus_tag="ACHKW0_004877"
     CDS             1635..2210
                     /locus_tag="ACHKW0_004877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO7477537.1"
                     /translation="MPLTSGIGSHSFNLGLEVFRARIAANGRGDITVGGETVSIVYDA
                     TDGSFSSSGGNGGLLSELLILGFNNGPRALSERMLSMLSDSGEAQSQEGIQSKISQYK
                     FSVCPERLQCPPESAQCPITLEIPEEGVFIKNSGDSVVCSLFDVTAFSRLVSEKSPHP
                     LTREKLTASMVVSADKCFYDHGKGSFVIKDS"
     gene            complement(2547..3785)
                     /gene="nleA"
                     /locus_tag="ACHKW0_004878"
     CDS             complement(2547..3785)
                     /gene="nleA"
                     /locus_tag="ACHKW0_004878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector NleA"
                     /protein_id="HFO7477538.1"
                     /translation="MNVQPNVHTGLTPPLHLAHPPHPAPNQIPESELPPGCETGFVVN
                     IPEDIQQHTPECGETTALLGLIRDKGLLSGLDAYLAPHLEEGSAGKKALDMFGLFNVT
                     QMALEIPSTVPGISGKYGVQMNIVKPDIHPTTGNYFLQLFPLHDEVGINFGALPGPLQ
                     KFLSQNPNPWFGVTAQVVRNHGIELPIVKTDNGWKLVGETPLTPEGPKANYTEEWVIR
                     PGEFDLKYGSTPLQSTLGLEFGAHFKWDLDNPNTKYAVLTNALANAAGAAGGYTLSQY
                     TGTDAMLSPHVGSMAGQALGHAVQCYTPGLKPDTILWWAGTTLGLADLNKAETGEGRF
                     TDYPRIWWHAREGAIFPNKEDIARATGADIRAMEEGIPVGQRHPNPEDVVIDIESNGL
                     PHHNPSNHVDIFDIIQETRV"
BASE COUNT         1165 a          727 c          879 g         1266 t
ORIGIN      
        1 aagatcttat tagtgtggta ttaagtaaaa tttagtaatt ttatccagtg tagtggattt
       61 gttgcatgga tggagttggt aaaaagtggt gtggctggca atccaggccg cgtcacagaa
      121 atggacaatg ccgctgagag actggcgaat ggcatacagg caccagaaaa atttcagaca
      181 tgacataatg cccatgccag aagttccttt tgcacctcgc ctgtatttat agataaacat
      241 agagtgaatg aatgagatat gaaagacatt acccttcccc ccccgacgtc cgcgtcctgt
      301 ctgacagggg ccatatctgt aaatactgaa gctgtattat ctcccatgca acacacttca
      361 gccttacatg taagagattt tgcttccctg tgctcacaga acctcaaagc taatgtattg
      421 ctaaatagtg atgaccacga agtacctata catcagaaaa atcctgctgc aataatgcaa
      481 aatatcgact ctaacatcaa acagatggca acagactggg ggatgtcgat tgaggaggtt
      541 gaggttatta tagggcgaga gaaaggcatt gtggaaccct cctgcggagt taccgctaat
      601 gctattatga aactatttct ggacaaggat ggcttcagtt actgctttga aaatgaacag
      661 acactatcgc tcgagcagct tcaggaacgc ctgtcctgta tgcctgaatg taagagcttt
      721 gtattacgtg ttaatgatgg tgcgcttggt catgcttaca ttgtcgatat tcccaaagga
      781 gaaaactctt gtcgtcctgc attcttgtat cagtcagatt taggagaggg cgtcaccaga
      841 aagttaagat ttgaggactg gatgacgcat aaagcattga ctccgatttt gctggatgat
      901 atttgtaatt acttctcctg catgtctcaa aataagacag atttggagca gattgcaacg
      961 ttatttgata ttgatgggaa tgttaaaatg ttacgaaagg aaaatattca atatcaaaag
     1021 catgacaatt ttagtttcca gttgtttgag tatgacaccg ataatattga aaaaaaacat
     1081 tgagataata aaatcactat gtagttagct tttgttaaaa ttgcaattat ttgtaaacat
     1141 aataataagt attatgacgt taaaatttaa ccatacggta aaatgattca ggaaaaccgg
     1201 ttcagcatag cctaggctga acttttgagg atatcaacga acggaatttt tgaacattta
     1261 tgagtagatc gttgttgaag ggcttcaatg agcatgtcaa gttcatcaac tgctggtcta
     1321 cgattacgac ggtttgattt accaatcaaa gcaagtttaa gtagatatgg acgagcactt
     1381 ttcgccgggt ttgatgtgta attaattccg tatataggtt tggctgcatc cagaacactg
     1441 ccaagataac taacatcgtg actgactgtt gctggaccta caaatgccaa tcagacggaa
     1501 tgttgaaagg atagaatgaa cagcccgaca cacaaaaaac ggagtccggc tccggttttt
     1561 gtgttgccat ataaggcaga tgtttgttac agctatttaa gtctggagtt caaattaaaa
     1621 tagggagttt tgttatgcca ttaacctcag gtattggatc acattcattt aatcttgggc
     1681 tggaggtttt tcgtgcccga attgcagcca atgggcgcgg agatattaca gtaggtggtg
     1741 aaactgtcag tattgtgtat gatgctactg atgggagctt ttcatccagt ggcggtaatg
     1801 gcggattgct ttctgagtta ttgattttgg gatttaataa tggtcctcga gcccttagtg
     1861 agagaatgct aagtatgctt tcggactcag gtgaagcaca atcgcaagag ggtattcaga
     1921 gcaaaatatc tcaatataag ttttctgttt gtccagagag acttcagtgc ccgccagagt
     1981 ctgctcaatg tcccattaca ttggagatac cagaggaagg ggtgtttatt aagaattcag
     2041 gggattcagt tgtatgtagt ttatttgatg ttactgcatt ttctcggtta gttagtgaaa
     2101 aatcacctca cccactgaca cgagagaaac taacggcatc aatggttgta agtgctgata
     2161 aatgttttta tgaccatggt aagggtagct tcgttataaa agatagttaa aatatttttc
     2221 tctgagcgaa tattagctgt cagtaatttg taaaaacgcc acaggcatca agaatacaag
     2281 agtatgcctg tggttgacag gtttcagatg acttgagggc gtctttacgt aaatgcattt
     2341 ttctgtggag atgctgtcgg aaaatattgg gttaaaaaca gaatccaagt caaatagtaa
     2401 gtttgttttt ttgagattag gtgtgtgtat ttagaatttt tatggtgtgt ttattttgtg
     2461 gggtatttta gaaaaaatat attaatcctt attaataata gctgccatcc atttcagcta
     2521 ttattttaaa ataaacaagt taaagcttag actcttgttt cttggattat atcaaagata
     2581 tcaacatgat ttgatggatt atgatgtggt aagccattgc tttcgatatc gattaccaca
     2641 tcctctggat ttggatgccg ctgtccaaca gggatacctt cttccatagc tcgtatatca
     2701 gcccctgttg cacgggcaat atcttcttta ttagggaaga tggcaccttc tcttgcgtgc
     2761 caccatatac gaggatagtc agtgaatctg ccttcacctg tctcggcttt gtttaaatct
     2821 gccagcccca gagtcgtacc tgcccaccat aaaatagtgt ctggctttaa tcctggagtg
     2881 tagcattgta cagcatgccc caaagcctgt ccggccatcg agccaacatg ggggctcagc
     2941 attgcgtctg ttcctgtata ttgagataga gtatatcccc ctgcagcacc agctgcattc
     3001 gcaagtgcat tggtaagaac ggcatatttg gtattaggat tatctaaatc ccatttaaaa
     3061 tgagctccaa attctaaacc taaagtgctc tgcaacggag tgctgccata ctttaagtca
     3121 aactcaccag gtctgatgac ccattcttcc gtataatttg ctttaggccc ttcaggtgta
     3181 agtggtgttt ctccaactag tttccatcca ttatctgttt tgactatagg tagttctatg
     3241 ccgtggttac gaacaacttg agctgttact ccaaaccatg gattgggatt ttgagagaga
     3301 aacttctgga gtggtcctgg gagcgcaccg aagtttattc ccacctcatc atgtaggggg
     3361 aataactgta aaaaataatt cccggttgtg gggtgaatat ctggttttac aatgttcatc
     3421 tgaacaccat atttaccaga gataccagga acggtgctgg gtatttctaa tgccatctga
     3481 gtaacattga ataaaccaaa catatccaat gctttttttc ctgctgagcc ttcttcaagg
     3541 tgaggagcaa gatatgcgtc taaccctgag agcagacctt tatctcttat taaacccaac
     3601 agagctgttg tttcaccgca ttccggagtg tgttgctgta tatcctctgg aatattaaca
     3661 acaaatccag tctcacatcc aggtggcaat tctgattccg gtatctgatt tggcgcagga
     3721 tgtggtgggt gtgcaagatg taaggggggg gttaatccag tgtgcacatt tggttgaaca
     3781 ttcataataa atccttatta catagtttat atccattaat aatatcaatc cattgatact
     3841 attagtgtca catatccgat gtggacagct ttaatatata gctgtttgaa tgcttgattg
     3901 atgtgactta gatctcaaaa ttaagttgtt aaatttacca gtttatctct tgaatactta
     3961 aaataaatta taaatgtttc tgttattgtt tttttatagt ttaagttatt gttggttgta
     4021 taagctgatt acatttt
//
LOCUS       DAZFXT010000235         4019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.235, whole genome shotgun sequence.
ACCESSION   DAZFXT010000235 DAZFXT010000000
VERSION     DAZFXT010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.235"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..859)
                     /locus_tag="ACHKW0_004879"
                     /pseudo
     CDS             complement(1..859)
                     /locus_tag="ACHKW0_004879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1001..2908)
                     /locus_tag="ACHKW0_004880"
                     /pseudo
     CDS             complement(1001..2908)
                     /locus_tag="ACHKW0_004880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308632.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
     gene            complement(2928..3389)
                     /locus_tag="ACHKW0_004881"
     CDS             complement(2928..3389)
                     /locus_tag="ACHKW0_004881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HFO7477539.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(3417..>4019)
                     /locus_tag="ACHKW0_004882"
     CDS             complement(3417..>4019)
                     /locus_tag="ACHKW0_004882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477540.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYMGS
                     GFNELKFDDATGREQVYIHAQKNMDTEVLNDRTTTVKHDHRETVKNDQTVTIQEGNRL
                     LTVEKGHKITGVLKGSLSEDVFQDRSTIAGSVHVDAVNNGGEGDGIQAYTAIKEILLA
                     VEESKIALTPDGIQLQVGESTVIRLSKDGITIVGGSVFIN"
BASE COUNT          841 a         1109 c          984 g         1085 t
ORIGIN      
        1 aactgatata atatctgacc gtcatttctg gctctttctt ttgttctgct attatggacc
       61 gaaaggagac tgccacgcat aatttcttca gccctttcca ttcaaacgtg aaatcaataa
      121 gttcatcagg gacatcgcaa acaatatgaa gacggatttc ttctctgccg tgactctttt
      181 cactcattgc gtaactgtca tgctctggat tatttaattc tttcagcgga aatttttcct
      241 caaaggcttt atttagccgc ccctggtttc cttttaccgc gaataaataa tcacctccct
      301 gtttttgtat cttttctgca atatctttct ggcagcccat cgcatcagtt gtgatgattt
      361 ttcctttaat atccatcatg ttaagaagtt caggaatggc tgtaatctca ttggatttct
      421 catccgtctt gatctgtccg aggaccagac tatgcattgt tgagaacgca ctaatgacat
      481 gaatcgctcc cctgcgacga ctcttgtcat aagagtgccg gagcgttttt ccatcaattg
      541 caatgacgtc tttatcatct gaagaatggc agtcacgcat ccagttaata aagcactcgt
      601 gaaattttgc aggactgata caggatacaa ctctggcaat gatatcgtga aaaggaatac
      661 cattttcaaa atcaccatat cacttcaaaa catcgggatg tgtttcccca aaatcctcta
      721 tatcttccca gccttctgca ccagaaataa cggcacaaat agtcaacagt agaatatccg
      781 ataatttatg ttccactttc caggcttgtc tgtaatcggg gataatagaa atatgttcca
      841 tcaatttttt aagttccatt ttgttctcct taattatgta aggagtattt gatcatgtat
      901 aagcaataaa aaacagcttc aggtaataag gaatatctca atttttaaac ataaaatgcg
      961 aattatttag tacaaaaaac cggcgttaca tgatctttcc ctggctccac gtctggtcgt
     1021 tatcggacca gcggatgatc cggtccgcag cgtcgtactc ataaaacagg tggtagtcca
     1081 gccgcgcatc cgcttcggtc agccgccccc gcgcatcctg ttcgtaggtc gccagcgtct
     1141 gtatgccgtt atccgtgcgg tgaatggctt tcaggtagcc gctggcgtgg tacgtcagtt
     1201 taagccggat cccgtcggtg tgactgacac ccgtgatccg gtactggctg tcgcgctcaa
     1261 acgtgatgcg gttgctggcg gtgtcgctca tcccgcccag catgtacatc cggtcattgg
     1321 ggctgtgtgc caggcggtca tcgctggtat cgtcagtcac cgggtggcgc agacgcattc
     1381 ccggaaaggc gtcataaaag aagcggctgc tcagtgcatc cctgtcgaac agctccagat
     1441 aatcatcgcg gcggcgcaga atgtactggg cgtgatacgg gttaaccgtc gtatcactgt
     1501 caccgtcgaa ggcaaatctc agcgtggctc cctggctgat gatatccacc cggtttccct
     1561 gttcacgcac ccaggcgtat tcgctccagg agtcgctcca gcccacgccc gtcaggccat
     1621 gcagcggcag tacagaactg tggaaacggg tgaaggagag ggggagggtc tggcccaggg
     1681 tgaagtcggt acgttcgtcg cagtacgcgc cggttaccgg atcgacagga tctgttaccc
     1741 atctgatgac tttattggca atacttttta acgacttacc tgttttcccc agcaacgcgc
     1801 cgagctttgc gcccagcagc ttcccgcttt tccccagcag gtttcccagt ttgcccagtt
     1861 tcgccagatt ccccagtttt cccagcagtc ctgctggcgg cagaagatag ctggctatcg
     1921 ttattcctcc caggatcatt ctctgccatg gcgggaactc cggctggata tccagatagg
     1981 tgacctgctc acccccaata aagacgtccg gcgaaccttc cacgatcgcc gcactgcact
     2041 ccagtttgtc gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctactgccct
     2101 gggctatcct cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg
     2161 ggaaatccac ctcttttttg atttcgccac gcccgcccac catggtggca aactccatta
     2221 ataccggacc ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg
     2281 cattgctttc cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt
     2341 tttccgctgc cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg
     2401 ggatttcagg tattgctcca ggtgggggag gagggggaag ggtgacggcg gcccttgcag
     2461 cgggctttcc gttaatgttt acattttcag aaccggtatt gatggcgccg tccgtgtcac
     2521 aaaagctgtt tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct
     2581 tatagctggc atacatcagc gcaggcacca gaaccagcgc ataagggccg gtggcgcagg
     2641 cggccataat caggcaacct tctgcgatgg tcactatcgc ccccagcacg gcccctgcca
     2701 gtgcccccag aaaactttta tgctgaattt catcgcccag cctggcggca ggtgggccgg
     2761 acttctttgc tgccagcatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc
     2821 tggcgaccat cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc
     2881 cctgtggccc gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg
     2941 tccttttgga tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc
     3001 atcgtctttt gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta
     3061 tctccacgcc gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc
     3121 ggaccgttcc aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg
     3181 caggcatgtt cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac
     3241 agctctggtg tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca
     3301 ctgttatctg tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga
     3361 atgtggcctt cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag
     3421 ttgatgaaaa cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc
     3481 gattccccga cctgtagctg aatgccatcc ggcgtcagcg caattttgct ttcctccacg
     3541 gccagcaaaa tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg
     3601 tttacagcgt caacatgcac cgaaccggca atcgtgcttc tgtcctgaaa gacatcctca
     3661 gataaagacc ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg
     3721 ttaccttcct ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt
     3781 acggtggtgg tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag
     3841 acctgttctc tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac
     3901 gttttggagc ggatggtcat ctgcgtcttt gttcccggca ggctgccggg ggtgcggttt
     3961 tcctggtggt aggtgcgccc cataatgatc ggctggtccg gatcgccgtt gaggaagtc
//
LOCUS       DAZFXT010000236         4001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.236, whole genome shotgun sequence.
ACCESSION   DAZFXT010000236 DAZFXT010000000
VERSION     DAZFXT010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.236"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(620..817)
                     /locus_tag="ACHKW0_004883"
     CDS             complement(620..817)
                     /locus_tag="ACHKW0_004883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7477541.1"
                     /translation="MLTTTSHDSVFLRADNSLIDMNYITSFTGMTDKWFYKLISEGHF
                     PKPIKLGRSSRWYKSEVEQWK"
     gene            complement(883..1153)
                     /locus_tag="ACHKW0_004884"
                     /pseudo
     CDS             complement(883..1153)
                     /locus_tag="ACHKW0_004884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593693.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1458..1646
                     /locus_tag="ACHKW0_004885"
     CDS             1458..1646
                     /locus_tag="ACHKW0_004885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477542.1"
                     /translation="MTNEELQAIALSMLERQREKVHQQRESLLNPSPFTRPKRLELEA
                     FLDKYPRRLKSGKTRPPG"
     gene            1974..2168
                     /locus_tag="ACHKW0_004886"
     CDS             1974..2168
                     /locus_tag="ACHKW0_004886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477543.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
     gene            2511..2639
                     /locus_tag="ACHKW0_004887"
     CDS             2511..2639
                     /locus_tag="ACHKW0_004887"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477544.1"
                     /translation="MGADVTEFSVQGKSGTCRQFSDLFNREIISYSLSERSVMEHR"
     gene            complement(2949..3284)
                     /locus_tag="ACHKW0_004888"
     CDS             complement(2949..3284)
                     /locus_tag="ACHKW0_004888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477545.1"
                     /translation="MVISTVGYEKRTGSASSASIIIQKELQPDNAIIQGKAISSVGRN
                     VEISTGKRGTDINTRRIPASNCLSARNAELKHNHTGARILWSYAFSIVTTNRNKNLAG
                     ANIKRCDTV"
     gene            complement(3918..>4001)
                     /locus_tag="ACHKW0_004889"
     CDS             complement(3918..>4001)
                     /locus_tag="ACHKW0_004889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477546.1"
                     /translation="LQGKVVWLWILPFVIEWLVVCTLGVNS"
BASE COUNT         1247 a          878 c          841 g         1035 t
ORIGIN      
        1 ctggtagaga ttcaggatac agtttgtagg agctttcttt tttgaacaat gatttgcagg
       61 acagttatgc taaactagcg gaatcagtat cacaacaata aatgactgat catacctgaa
      121 atttgaatta acaggcatca tgaatgtcaa tagtctaagc ggtgactact tatatcgctt
      181 gcataaatac taaacactga ctagaaaagt attggataac cccgtcacta aaagtaacgc
      241 ttcggttatt tgctcggaat gtattaacgc caagattgtt agccgttacg ccttatatca
      301 ctggcgctga caaatgtttc ttttctaatt tactgaaccg gcgcagacac gcaggaaagc
      361 acgccggcac cggtgtaatg ccagttctaa tcaataagaa tggaatgacg aatcagtcag
      421 gaagggataa caacccgctg ctgaacggaa ggtatcagca ggaagatgaa cgggaatatc
      481 gccagcacca agggcagggc cgggttccac aataccagcg ctggcatttt gcacattgcc
      541 agcgtaccgg cattacaaca cgtttcatgc tgctgccccc caggattcct cgattcgttg
      601 ctacatccac tgctctactt cacttccact gctccacttc acttttgtac cagcggctgc
      661 tgcgccccag tttgatgggt ttagggaaat ggccttcact gatcaacttg taaaaccatt
      721 tgtcggtcat accggtgaaa ctggtgatat agttcatgtc gatcagggaa ttatcggcac
      781 gcagaaatac gctgtcgtgg cttgttgtag tcaacatggt cagaatgtcc tgtgagattt
      841 atgaagattg tgcgaatgcg ggaaatctac tgagctgtgc tttcagaact ggcctgttac
      901 gggataccag ggattaccgg cggggtaacg ggcttccgga tcatacacac cacgattatc
      961 gcggacaaaa tcactgaacg cccatatcac ctctttaagt atgtcttcgc agtccggtaa
     1021 atgacgatcc agcgccacat cccgggtggt acagctttga tgcgcccggt gacacaaagc
     1081 ccggattgtt ccagacatcc tgaatcaaac gccccagatt aggggcgtcg aaatatgcct
     1141 ctctgaccat tatattccgg cgtataggta gcaggtctga agtgacaatg cgtcacctga
     1201 cgttaaaagt cacccaagat gaccccagtg ggggcaacaa gtataaaacc gttttttgat
     1261 taagtggtcg gatatgaaaa ggggaaggtt tatgtgaaaa atacctgtgt tttctttgaa
     1321 aacacacaac agtatcaggt tatttatgtt tatacagtgc gttccaaact gacctgaata
     1381 attacgcaag ttgcaacaat aaaacatttt tgccgtagtg tgaggtactc cggtaaattg
     1441 cttcccaggt gcagacaatg actaacgaag aactacaggc aatcgcatta tccatgcttg
     1501 agcgccagcg cgaaaaagta caccagcaac gagagtcttt gcttaaccca tctccattca
     1561 ccaggccaaa acgattggag cttgaagcat tcttggacaa atacccacgg cggcttaaat
     1621 caggcaaaac cagaccacca ggctgatagc tcacagtttg ttctccgatt caataaaacg
     1681 gataagaacc atcagcgcgg tagcgtaagg cactacagcg cattgcttta cgagtaataa
     1741 caatggattt atcaccagaa gacgcaaagt ttttacgaga actgcaacac cgtatgctca
     1801 gagaagccat tatcgaacag caaacgggca tgagcgccat agacccattg cgatacaaac
     1861 gggcctcatc tcctttagtt gacgacaatt ccaatgcgga taaaacacgt ccaccacact
     1921 gattacattt ttcattagca cagtggtgtg atgcagcata cgaggaaaac cggatgaatg
     1981 agaacaaaat aaaaaggctg gagcaactgt tacaggcacg ccaacgggag tttgccacta
     2041 aatggggacg tgccgcgctg ccacatgaac gcctaaccta ttttccactt gaagacctca
     2101 ttaaaaataa tggcaaaaaa atcaggtctg ataagaaaca cttcttagaa tgcaatgaag
     2161 acaaataaca acaatctgca tattgccacc aaccacagat gtggcatcgt tatcagcgca
     2221 cttgattttc tttatatcca ctgacgagtt tgccggtcat tctggtatca tgcagatatt
     2281 tacggcgtac aagcacacta gcatgacatt gtaataaggc ctgagcctaa cgctttaatg
     2341 tgtgtcatac actcagtggc gtaatcgttg acaatatggt gcatgtaatc gtaagcgtcc
     2401 agcgaaatcc atctggtcat aatctgaagc atccgacaaa gtggtgtcca ccaaataagt
     2461 gatctgcgcc caatatcctg agccggaact ttgacaaagg ccaacgaaaa atgggagcgg
     2521 atgttacaga gttctcggta caggggaaaa gtggtacctg tcgccagttc tccgatctgt
     2581 ttaaccggga aattatctcc tacagcttgt cagaaaggtc ggtgatggag catcgttaat
     2641 acgatgctat acgatgcatt cacagtgccc ggccagagga tgccccgtcg ctgcatatgg
     2701 atcagggttg gcaatatcga attgcaggct ataggcaaag ttaaagcccc atggagtagc
     2761 acaaaatatg ccgcacaaag gaaacggtct gaataacgca gtgatgaaga acttcttcag
     2821 cacactgcta aaacgcatag tgatcgagcg ctgattctgg cgaacaactg aactaataca
     2881 tcagaatctg cattatgtta aataaatata aaaagatggt ttaaataccc cgttactttt
     2941 gacttacact atacggtatc gcatcgttta atattcgcac cggccagatt tttatttcta
     3001 ttagttgtca caatactgaa tgcgtacgac cacagtattc tggctcctgt gtggttatgc
     3061 tttaattctg cgttccgggc agataagcag ttgcttgcag gaatccttct tgtgttaatg
     3121 tcagttcccc ttttaccagt gctgatttcc acattccgtc caacagagct tatagccttt
     3181 ccctggatta tagcattgtc cggctgaagt tctttttgaa taataataga agcactgctg
     3241 gcagatccag tccgtttttc ataacccact gtactgataa ccataatcta atcagtagaa
     3301 attgagtcga aaataagcac tactccatac aggataatta gaggtcagtt tgattattca
     3361 caattcatca tcagcatttt ctatttctga cgaaatcaat atgaaaataa ccatatatga
     3421 taattattat aataacggct ttaattggaa tacatatatt acaacgtatt atatataatt
     3481 ggtattctgg gaactatatt ctcaaaatac agtagaaacg aggtatgttt ctggtggaaa
     3541 ggacagtggg attaaaaagt aagaattgat aaaaaaaacg ccagcaacac atagtgccgg
     3601 cggagggaat accccatgga gaaaatgtga tgcctagagc atcatgaata tataattaaa
     3661 aatagttagc gttgtcaata ctttcacaaa aataattttc gtagtataga aagatatttt
     3721 tatgcatgac ctacctgaat ttgctccggg tagaggttat aaataaaaat tgaatcacga
     3781 caaacacaat attcatagta tggcgatgcc tacgccagca agaatagcgc caataatatt
     3841 gggaatataa tagacaccag acgcacaggc atctcactcc ttaacaaaca acaatcagga
     3901 tatttacttt taccaagcta actgtttaca cccaaagtac acacaactaa ccattcaata
     3961 acaaatggca atatccatag ccatacgact ttaccttgta a
//
LOCUS       DAZFXT010000237         3708 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.238, whole genome shotgun sequence.
ACCESSION   DAZFXT010000237 DAZFXT010000000
VERSION     DAZFXT010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.238"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..105)
                     /locus_tag="ACHKW0_004890"
     CDS             complement(<1..105)
                     /locus_tag="ACHKW0_004890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477547.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKE"
     gene            complement(172..837)
                     /gene="yieH"
                     /locus_tag="ACHKW0_004891"
     CDS             complement(172..837)
                     /gene="yieH"
                     /locus_tag="ACHKW0_004891"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HFO7477548.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            1004..2341
                     /gene="adeP"
                     /locus_tag="ACHKW0_004892"
     CDS             1004..2341
                     /gene="adeP"
                     /locus_tag="ACHKW0_004892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HFO7477549.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            complement(2395..2961)
                     /gene="yieF"
                     /locus_tag="ACHKW0_004893"
                     /gene_synonym="chrR"
     CDS             complement(2395..2961)
                     /gene="yieF"
                     /locus_tag="ACHKW0_004893"
                     /gene_synonym="chrR"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HFO7477550.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(2983..>3708)
                     /gene="yieE"
                     /locus_tag="ACHKW0_004894"
     CDS             complement(2983..>3708)
                     /gene="yieE"
                     /locus_tag="ACHKW0_004894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HFO7477551.1"
                     /translation="ILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRGLLAELMFM
                     LYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLDMELQRATR
                     GFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLNDDPRDLQL
                     LPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWKSLPDIHSR
                     ANNPTSRMMRFAQLSTVKAFSPN"
BASE COUNT          829 a          938 c          954 g          987 t
ORIGIN      
        1 ttcttttgcg aagaatatgt agaggacaag aaacagaaca ccaattaacg gtcctaaaac
       61 cgcaaaataa agtccgtgat gtattacccg acgtgaaaca gacatatcaa actcccttta
      121 cttacctttg agcgccaatt taagcggggt aagtgaaggg agtgtgaaga actatgccgt
      181 aatatcccaa ccacgcgctt tccacagttc cggtaactgc gaaagatggg taaaggtggt
      241 gactttcggg tgaacgatcg gcttattgtg cgggtcggcg cagaagtaga acacttccat
      301 acctgcgtcg ataccagatt gcgcaccggc aactgagtca tcaaccagaa tgcagttttc
      361 tacatttaca ttcatcgctt ttgccgcatg gaacattaac gccgggtctg gcttccagcg
      421 ctgaatatcg tagccgctga acagtttatc cgggaagtag tgcaacatat tcagcttgcc
      481 catagaatgc tgcattttgt tatttgggcc gttagatacc acacacattg gcgcagtgat
      541 cgctgacagg agcgctccag ccccttcgat ggcttccagt tctgaatcga acagccgagc
      601 gacttctgca cggtaaacgt gttcagcttc tgttttcgct aacgtaacac catgttcaag
      661 ggaaacaata tcgataattt cgtacagttt tacacctttg aaacgtttga atacctcttc
      721 aggatcgagc gtaataccaa attcctgaaa catcgttaca tatgcgcggg agcaaatgac
      781 ttcactgtcg accagcgtac cgtcgcagtc gaaaaatacc gcttctatct gggacatgtc
      841 tttcctttat cgttagttta acgttttcgt catgccaatt cgcatgacgc aatcgttgcc
      901 gtataagcaa atacaatgaa aaaaagttat cttcagccgt ccatattgtc tcattttggt
      961 atacgatagc gacggatttt ccctccttgt tcggaaatag ataatgagtc aacaacacac
     1021 aacccaggct tctggccagg ggatgctgga acgcgtgttt aaactgcgcg aacatggcac
     1081 gacggcacgg accgaagtga tcgccggttt taccaccttc ctgacgatgg tttacatcgt
     1141 ttttgttaac ccgcaaattc ttggcgttgc tggcatggat accagcgccg tcttcgtcac
     1201 tacctgtctg atcgctgcat tcggcagtat tatgatggga ctgtttgcta acctgccagt
     1261 tgcactggca cccgctatgg gcctgaatgc gttcttcgct tttgtcgttg tacaggcgat
     1321 gggcttgccg tggcaagtcg ggatgggcgc aatcttctgg ggtgcgattg gtctgctgtt
     1381 actgacgatt ttccgcgttc gctactggat gattgccaac attccggtaa gtctgcgtgt
     1441 gggcatcacc agcggtatcg gtctgttcat tggtatgatg ggactgaaga atgcaggtgt
     1501 gattgtcgct aacccggaaa cgctggtcag catcggtaac ctgacttctc acagcgtgct
     1561 gctggggatc ctcggcttct tcatcattgc tattctggcc tcgcgcaaca ttcacgcagc
     1621 ggtgctggtt tctatcgtgg tgacgacgct gctgggctgg atgctgggtg atgtgcacta
     1681 caatggcatc gtttctgcgc cgccgagcgt aatgacagtt gtgggtcatg tagatttagc
     1741 cgggtcgttt aacctcgggc tggcaggggt gattttctct ttcatgctgg tcaacctgtt
     1801 tgactcctcc ggtacgctga ttggcgtgac cgataaagca ggcctggcgg atgagaaggg
     1861 taaattcccg cgcatgaagc aggcgctgta tgtcgacagt atctcttccg tgaccggttc
     1921 gtttatcggt acttcttccg ttacggctta tattgagtcc tcttccggcg tatcggttgg
     1981 cggtcgtacc ggtctgacgg cagtggttgt tggtctgctg ttcctgctgg ttatctttct
     2041 gtcgccgctg gcggggatgg tgccaggcta cgctgcagct ggtgcgctga tttacgttgg
     2101 cgtgctgatg acttccagtc tggcacgcgt gaactggcag gatcttactg aatctgttcc
     2161 ggcgtttatt accgccgtga tgatgccgtt cagcttctcg attaccgaag gtatcgcgct
     2221 gggctttatc tcctactgcg tgatgaaaat cggtaccgga cgtctgcgtg atctaagccc
     2281 gtgcgtaatc atcgttgcgc tgctgtttat cctgaagatt gtctttatcg acgctcacta
     2341 aaatcgatag cgcgttaaaa acccgccatg catgattgct ggcgggtttt ttatttagat
     2401 cttaactcgc tgaataaact caccaaatgc ggtcaattgc ccggtcaggt ggtccagcgt
     2461 accctgatca atcacttctc cggtttgcgg atcaactttg ttctgaatca cgccgcccat
     2521 aaattccggc ttgttcatca ccattgcatc gaggaaaacc agaatctggc gcaggtgata
     2581 ctgacagcgc gcgccgccaa tcacgcccat tgagctggtc tgaatcaaca ccggtttacc
     2641 ggccagcggt tgatccggca ggcgggaaag ccagtcgatg gcatttttca gcccacccgg
     2701 taccgagtag ttatattccg gcgtgacgat caccacaccg tcagcctgac ggatctgttc
     2761 cgccagagct tcaaccgttg ctggaaaacc ttcttcctgc tgtacgtcag cgtcatacaa
     2821 gggaatgtcg gcaatggaag gtaacgcatt gacttccatg ctcgccggag caattttcgg
     2881 cagggtacgt gcaaccatgc cattaaatga gcctttgcgc aggctcccca gtaacgtaac
     2941 cacctgcaat ttttcagaca tgatgactcc tgtggttgtc tatcaatttg gcgaaaaagc
     3001 cttcacggta gagagctggg caaaacgcat catccggctg gtaggattgt tggcgcggct
     3061 gtgaatatcc ggcaggcttt tccagccgtg tttgccatcg aactcccaga cactgagctt
     3121 ttcaatcgtg ggcgtgaccg ccacggacca caccagcacg tcctccgcgt cgcataacgc
     3181 ttctacatga tttacatgag cacatttcag gcgtccggca atgggcagca gctgcagatc
     3241 gcgcggatcg tcattcaaaa catcaccggt tagttttagc acgcttcggc gcagcgtgat
     3301 gagctgcgcc cgcgcttcgt taggatcgtt ttgtttactg atccatagcg attcattgct
     3361 ggaaaaggtg tggttatcgg gcgcgtgtgg gctgtgaaac ccgcgcgtcg cacgctgtag
     3421 ttccatatcg agaccacatt caccttcggt tgttaacgcc acgccaacca tattcccggc
     3481 ataggaaata gaaaacgaag gcaaattttt atcactgaaa accggtttac ctttcggcag
     3541 ggtgacgatt tccggcaatt cgccaatgcc atacagcatg aacatcagtt ctgcgagtaa
     3601 acctctagac gccagaaaac ggctttgacg atgaggggga atgtttcggg cttcttgatg
     3661 acactgggag gggagacgaa cagaaatcag atgtccttcc gttaaaat
//
LOCUS       DAZFXT010000238         3679 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.239, whole genome shotgun sequence.
ACCESSION   DAZFXT010000238 DAZFXT010000000
VERSION     DAZFXT010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3679
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.239"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..559
                     /locus_tag="ACHKW0_004895"
     CDS             <1..559
                     /locus_tag="ACHKW0_004895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477552.1"
                     /translation="SAVFGSNFTVGLTGDTEFLDSAIIGPVGNSIKCYYVSSLSGFYY
                     TPAYVFADVEFSMRQYIKCSHLTNKYGDDNLMYVFCKGGYEFDVNYLCVMASDLDNIK
                     NTGDFPKITEDSPKASSKRNEVIYSLIKLIPEMGDVDLDTESLPKIADLIDSIAASRG
                     IEFPKTHWQTWQKYLGRESQQKRKK"
     gene            651..875
                     /locus_tag="ACHKW0_004896"
     CDS             651..875
                     /locus_tag="ACHKW0_004896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7477553.1"
                     /translation="MNQNTQFQSLSDRVIREDECRKLTGVCRTTRYELEKKGNFPSRR
                     NLGGRSVGWLLSEVMEWVKSRDRINSGKAA"
     gene            complement(1010..1189)
                     /locus_tag="ACHKW0_004897"
     CDS             complement(1010..1189)
                     /locus_tag="ACHKW0_004897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7477554.1"
                     /translation="MATKAVNGKSKKLEARVPHAIADAVENLKEEGESTGQFIVSALE
                     GEIKRRQRRKAKEQE"
     gene            1364..1567
                     /locus_tag="ACHKW0_004898"
     CDS             1364..1567
                     /locus_tag="ACHKW0_004898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HFO7477555.1"
                     /translation="MRNHTTHPQGRDSHNLNKYIWRFLALSAGESQIIHVTAWTEREA
                     RSRCPSGCVAVFVARIRQGVCRA"
     gene            1560..1733
                     /locus_tag="ACHKW0_004899"
                     /pseudo
     CDS             1560..1733
                     /locus_tag="ACHKW0_004899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920679.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1746..1937
                     /locus_tag="ACHKW0_004900"
     CDS             1746..1937
                     /locus_tag="ACHKW0_004900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477556.1"
                     /translation="MKEITLHEAAERAHQTEIICRLLEVYPNKLNDGDISALAGLLAK
                     LSGSVALWLIDERFKRYEQ"
     gene            1927..2169
                     /locus_tag="ACHKW0_004901"
     CDS             1927..2169
                     /locus_tag="ACHKW0_004901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001455401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477557.1"
                     /translation="MNNNKRVICELNELTQSAISYNNAVSEIINVWFESLTNCERDES
                     TTRLTFAISELLNISTSKIESISEKINGLSGHAIGE"
     gene            2175..2474
                     /locus_tag="ACHKW0_004902"
     CDS             2175..2474
                     /locus_tag="ACHKW0_004902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7477558.1"
                     /translation="MKLKYSGLTASGKTRTKFMCGDIYRDKYGGTVMIKGVEERRVTY
                     RREGYEYDCVMPVYQFNRDFSLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            2471..>3679
                     /locus_tag="ACHKW0_004903"
     CDS             2471..>3679
                     /locus_tag="ACHKW0_004903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5906 domain-containing protein"
                     /protein_id="HFO7477559.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWAHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELYALQIVPDGKKRVRLYQAGELDLVETKKIGQKLAAAGVQDA
                     KFYPEGMHGQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLADRFDG
                     VCVHPESEIVHVWRGGVWCPVSTMELSREMVAIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNAPNFH
                     KWLEHAAGKDPRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVL"
BASE COUNT         1063 a          779 c          941 g          896 t
ORIGIN      
        1 atctgctgtt tttggaagta attttacagt tggtttaact ggtgacactg aatttttaga
       61 tagtgccatt attggtcctg tagggaattc tatcaaatgt tattatgtta gcagcttatc
      121 agggttttat tatactccgg catatgtttt tgctgatgtc gaatttagca tgagacaata
      181 tataaaatgt tctcatctta cgaataagta tggtgatgat aatctcatgt atgttttttg
      241 taagggtggt tatgaatttg atgttaacta tttgtgtgtg atggctagtg atttagataa
      301 tataaaaaat acgggagact tcccgaagat aacagaagat agcccaaaag catcgtcaaa
      361 aaggaatgag gttatttact ctttaattaa acttattcca gaaatgggtg atgtagacct
      421 ggacacggaa tcattaccta aaatagcaga tttaattgat tctatagctg catccagagg
      481 gatagaattt ccaaaaacac attggcagac atggcaaaag tatttggggc gtgaatctca
      541 acaaaaacgt aaaaagtgat atcacgtgct ggtagtgtga tatcacacaa acggcgtttt
      601 actaccgcct agaatcgtcc tcgttcggca acgttcgagg acgttttttt atgaatcaaa
      661 atacacaatt tcaatcacta tcagatcgcg taatccgtga ggatgaatgc cgtaagttaa
      721 caggagtgtg ccgcactaca cgctacgaac tggagaaaaa agggaatttc ccgtcacgcc
      781 gtaatctggg tggtcgttca gtaggttggc tgctttccga ggttatggaa tgggttaaaa
      841 gccgggatcg tattaattca ggtaaggcag cgtaggggga acatatgaca cataaaacaa
      901 aggcgacctg tggcggtcgc caatgggaaa acactaaaca taagcccgaa catcatagcg
      961 atttgctgtc tggtgggcaa tgtgctcagt gtgctggctt gctggtggac tattcctgct
     1021 ctttggcctt gcggcgctgg cggcgtttga tctcgccttc aagtgccgaa acgataaatt
     1081 gcccagtgct ttcgccttct tcctttaaat tttctacggc gtcagctatt gcatggggga
     1141 ctcgtgcctc aagttttttg gattttccat taactgcttt cgttgccata tctgatttcc
     1201 ttgttattag gtggctgaca gtatacacac aagaaaatgg aaaaacacta ttgacgtggc
     1261 tgacaccttt attgaggtaa tcgatgaaaa gacaaagccc gcaggtgcta ccaacaccaa
     1321 cgggcttcta acaaccaacg atagcaacag tatcgaggta gctatgagaa atcataccac
     1381 acacccgcaa gggcgggact cgcacaacct gaataaatac atctggcgtt ttctggccct
     1441 gagtgccgga gaatctcaaa tcatccacgt aaccgcctgg acggaacgcg aagcgcgtag
     1501 ccgttgcccg tcaggttgtg ttgctgtatt tgtcgcccgt attcgtcagg gggtgtgccg
     1561 tgcttaaaac cttccgcgtt ttttcccgtg ctgttaacaa gcgcggctat accgttggca
     1621 tcacgcagaa cgtgaaggcc gttaatatgc aagcggcgat tgattcagta atcagtaatt
     1681 ccggtaaagc aggtttatca catgtaatta ttgcagcagt acatgaatta aaaggggggg
     1741 cattaatgaa ggaaattaca ttacacgaag ccgctgaacg tgcgcaccag acagaaatta
     1801 tttgccgact tcttgaggtg taccctaata aattaaatga tggtgatata tccgcattag
     1861 ctggccttct ggcaaaactt tctggaagtg ttgcgctgtg gttaattgat gagagattta
     1921 aacgctatga acaataacaa acgcgtaatt tgcgaattga atgaactcac acaaagcgca
     1981 atatcttaca acaatgccgt aagtgagatt attaacgtct ggtttgaatc gctgacaaat
     2041 tgcgagcgtg acgagtcaac gacaagatta acctttgcca tttctgaatt actgaatatt
     2101 tccaccagca aaatagagag catatcagag aagattaacg gcttatcagg tcacgctatc
     2161 ggagaataaa aataatgaaa cttaaatatt ctggcttaac tgccagcggc aaaactcgca
     2221 ctaaattcat gtgcggggat atttaccgcg ataagtacgg cggcacggta atgattaagg
     2281 gcgtggagga acggcgcgta acttaccgcc gtgaaggcta cgaatatgat tgcgttatgc
     2341 ctgtttatca gttcaaccgt gatttttctc tggtacaggc agcgccccgc agtaagccaa
     2401 ccagcaggga gaaagcacgc gccaatattc aggaaataaa aaagatgctt aacgtattca
     2461 ggggtaaaaa atgaaactgg caccgaacgt aaaaaaacag ccacgcggaa taaaacacaa
     2521 agacacagag gtgattattt tcgcgggtag tgatgcgtgg gcacatgcaa aacagtggca
     2581 ggagcaggac ggccccgcat ccggcgataa tgtgcctcct gtgtggcttg ggccaaatca
     2641 gcttgccgaa ctttatgcac tgcaaattgt tcctgatgga aaaaaacgcg taaggctgta
     2701 ccaggcagga gaactggact tggtggagac caaaaagatt ggtcagaagc tggcggcggc
     2761 aggcgtacag gatgcaaaat tttaccccga aggaatgcac ggtcagaagt gtgaaaactg
     2821 gcggcgctat ctgaatgctg agcgtgaaaa tattgccgca gggcttacca tgccggagca
     2881 gaaaaatacg caactggcac aaatggcaga cagtgagcgc gcccagctgc ttgccgatcg
     2941 ctttgatggc gtttgcgtac atcctgaaag tgaaatcgtt cacgtatggc gcggcggggt
     3001 atggtgtccg gtcagcacaa tggaacttag ccgcgaaatg gtggcgatct attcagagca
     3061 cagggccact ttcagcaaac gcgtgatcaa taacgccgtt gaagcgttaa aagttattgc
     3121 cgaaccaatg ggggagccgt ccggcgattt gctgccgttc gccaatggtg cacttgacct
     3181 gaaaacgggg gaattttccc cgcacacgcc ggagaactgg atcaccacgc acaacggcat
     3241 tgagtacacg ccaccagcac caggggagaa cattcgcgac aacgcgccaa actttcataa
     3301 atggcttgag cacgcagccg gaaaagaccc gcgcaagatg atgcgtatat gtgccgcgct
     3361 gtacatgatt atggcgaacc ggtacgactg gcagatgttt attgaggcca ccggagacgg
     3421 cgggagcggt aaaagtacat tcacacacat agccagcctt ctggcaggga aacagaacac
     3481 cgtaagcgcg gagatgacat cactcgatga tgcaggaggg cgcgcgcagg ttgtcgggag
     3541 tcgtcttatc gtcctggctg accagccgaa atatacaggc gaaggaacgg gcatcaagaa
     3601 aatcacgggc ggcgaccccg tggaaattaa cccgaaatat gaaaagcgtt tcacggcggt
     3661 aatcagggcg gtggtgctg
//
LOCUS       DAZFXT010000239         3591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.240, whole genome shotgun sequence.
ACCESSION   DAZFXT010000239 DAZFXT010000000
VERSION     DAZFXT010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.240"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..729
                     /locus_tag="ACHKW0_004904"
     CDS             <1..729
                     /locus_tag="ACHKW0_004904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdQ family protein"
                     /protein_id="HFO7477560.1"
                     /translation="AVALPENFRIHDLEKFNLNRFRFRGALSTASIDDFTRYSKDLAD
                     EGTRCFIDADNMRAVSVLNLGTIDEPGHADNTATLKLKKAAPFSALLSVNGERNSQKS
                     LAEWIEDWADYLVGFDANGDTIQATKAAAAVRKITIEANQTADFEDNDFSGKRSLMES
                     VEAKTKDIMPVAFEFKCVPFEGLKERPFKLRLSIITGDRPVLVLRIIQLEAVQEDMAN
                     EFRDLLVEKFKDSKVETFIGTFTA"
     gene            858..1394
                     /gene="yfdR"
                     /locus_tag="ACHKW0_004905"
     CDS             858..1394
                     /gene="yfdR"
                     /locus_tag="ACHKW0_004905"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HFO7477561.1"
                     /translation="MSFIKTFSGKHFYYDRINKDNIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNELSELRKCA"
     gene            1385..1747
                     /locus_tag="ACHKW0_004906"
     CDS             1385..1747
                     /locus_tag="ACHKW0_004906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001242743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477562.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LESKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPETKK"
     gene            1744..1959
                     /locus_tag="ACHKW0_004907"
     CDS             1744..1959
                     /locus_tag="ACHKW0_004907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477563.1"
                     /translation="MSVIKTHTGIVITRDGEKRMKLHSTETSWVAGRCESYDKKTGYR
                     WGAPNMRRRLLLDSIRPIKQVATREQN"
     gene            2019..2225
                     /locus_tag="ACHKW0_004908"
     CDS             2019..2225
                     /locus_tag="ACHKW0_004908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7477564.1"
                     /translation="MLNLDCVPISTYCKETGETPEAINKRVQRGVWREGVQVLKVEGV
                     KERWIDLSEVAKWARQNCSNYRAA"
     gene            2186..3361
                     /locus_tag="ACHKW0_004909"
     CDS             2186..3361
                     /locus_tag="ACHKW0_004909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HFO7477565.1"
                     /translation="MGQTKLLKLPRGVTIRKHRQGETINITFTYKGVRCREPLSNLEV
                     TPKNIKYAERTLGEIHNKIERGTFIYAEYFPRSARLKIFGNAAAGKTVKMYLDEYLEI
                     CETRKLSPSTIGGYKKCRSALASLHICPASELTPATLKAWIQSQKTTLKTIRNQLSFL
                     RSALDEAVTDGVLQINPVSLVTASRYQSDKSEAESSYVVDPLSPAEVDALLAAAGNKQ
                     WENLFRFAIHTGLRSSELCALRWRDIDFVGKTAHVQSASVVGVIKGTKTKAGTRKVEL
                     TEEAMLALINQKPFTFMKDATVFEDPKTNKPWASADAIRKKAWVPTLRKAGIRYRNPY
                     QTRHTYATKYISQGANLFWLAGQMGHKGPEMLFRHYGRYLSDYDNHSSTTLTSNCKK"
BASE COUNT         1079 a          782 c          837 g          893 t
ORIGIN      
        1 gcagtggcat tacctgaaaa tttccgcatt catgatctgg aaaaatttaa tttaaatcgc
       61 ttccgtttcc gtggtgcgct ttccactgcc agcatcgatg actttacccg ttattctaaa
      121 gatcttgcag atgaaggcac ccgctgcttt atcgatgctg ataatatgcg tgccgtcagt
      181 gtgcttaacc tgggtactat tgatgaacca ggtcacgcag ataacaccgc cactctcaaa
      241 ctgaaaaagg cagcgccgtt ctctgctctg ttgtctgtta atggcgagcg taactcccag
      301 aagtcactgg cagaatggat tgaagactgg gccgactacc ttgtgggctt tgatgctaat
      361 ggtgacacca ttcaggcaac aaaagcggct gcggcggtcc gtaaaatcac gattgaagca
      421 aaccagactg ctgattttga agataatgac ttcagcggca aacgctccct gatggagtct
      481 gtcgaagcga agaccaaaga cattatgcca gtggcatttg agtttaaatg cgttccgttt
      541 gaaggtctga aagaacgtcc gtttaaatta cgcctcagca ttatcactgg cgatcgtccg
      601 gtactggttc tacgcattat tcagctggaa gcggtgcagg aagatatggc taacgaattt
      661 cgtgatctgc ttgttgagaa attcaaggac agcaaagtag aaacctttat tgggactttc
      721 accgcctgat ttcattaccg caaatgcccc tgcgggggca tttatggaaa cgtaattgac
      781 tcaataatcg cctgaaggcg agggttttct ttaaccaaaa ttcagcgcgg tgcagcgcat
      841 ataacgtgga gaacaaaatg tcatttatta aaactttttc cgggaagcat ttttattatg
      901 acaggataaa taaagacaac atcgatatta acgatatcgc ggtttccctt tcaaatatct
      961 gtcgctttgc cggccatctt tcacacttct acagcgtcgc ccaacatgcg gtgctttgca
     1021 gccagctggt accgcaggaa tttgcttttg aagcgttaat gcatgatgca acagaagcgt
     1081 attgccagga catccccgca ccactgaaac gccttcttcc tgactataaa cggatggaag
     1141 aaaaaataga cgccgtaatc cgtgagaaat acgggttacc cccggttatg agcacgcctg
     1201 tgaaatatgc cgatctcatc atgctggcaa ccgaacgccg cgatctcggg cttgatgatg
     1261 gctctttctg gcctgtactg gaaggcatcc cggcaacaga gatgttcaac gtgattccac
     1321 tggcaccggg ccatgcctac gggatgttta tggaacgttt taacgagtta tcggagttac
     1381 gcaaatgcgc atgaatgttt tcgaaatgga agggtttctt cgtgggagat gtgtaccgcg
     1441 agatctgaaa gtgaacgaaa caaatgctga gtacctggta cgtaaattcg acgcgcttga
     1501 agctaaatgt gcggcactgg aaagcaaaat aataccagtg tcagctgaac tgcctccagc
     1561 aaatgaaagt gttctgttat ttgatgctaa cggagaaggc tggctgattg gctggcgttc
     1621 tctctggtac acctggggac aaaaagaaac cggagaatgg cagtggacat ttcaggtcgg
     1681 ggaccttgaa aacgtcaata tcactcactg ggcagtaatg ccgaaagcac cagagactaa
     1741 gaaatgagcg tgataaaaac tcatacagga attgttatca cccgagacgg tgaaaagcgg
     1801 atgaaattac attccactga aacgtcctgg gttgccggac gttgtgaatc ctacgacaaa
     1861 aagactggtt accgttgggg tgcacctaac atgcgtcgcc gtctgctact ggacagcatc
     1921 aggccaataa aacaagtagc aaccagagaa caaaattaat tatcaggact ggaatttgat
     1981 attactgccc atgtgcagcg ggctaagtgg agaaacatat gctgaacctc gattgtgttc
     2041 ctatctcaac ttattgcaaa gaaactggcg aaactcctga agcaataaac aaacgtgtac
     2101 agcgcggtgt ttggcgtgaa ggtgttcagg ttttaaaggt tgaaggcgtt aaggagaggt
     2161 ggattgatct tagtgaggtt gcaaaatggg ccagacaaaa ctgctcaaac taccgcgcgg
     2221 cgtaacaatc aggaaacacc gccagggcga aacgatcaat ataactttca cctacaaagg
     2281 agttagatgt cgtgagccgc tttccaatct ggaagtaaca ccaaagaaca ttaaatacgc
     2341 cgagcgtaca ctcggcgaaa tccataataa gatcgaaagg ggaacattca tttatgcaga
     2401 atattttccc cgttctgctc gtttgaaaat ttttggtaat gctgctgcag gcaaaacggt
     2461 aaagatgtac ctggacgaat atcttgaaat ctgcgaaaca agaaaacttt caccctctac
     2521 gattggtggc tataaaaaat gccgtagtgc gttagcctca cttcacattt gccctgcaag
     2581 tgaattaaca ccagcaaccc tgaaagcgtg gattcaaagc cagaaaacaa ccttaaaaac
     2641 cattcgcaac cagttatctt tcctgcggtc agcacttgat gaagccgtga ccgatggggt
     2701 acttcaaatt aaccccgtat cgttagtaac agcttcgcgc taccaaagtg ataagtcaga
     2761 agcagaaagc agctatgtgg ttgatccgct atcaccagca gaagttgatg cattactagc
     2821 agcagccgga aacaaacaat gggaaaatct gttccggttc gctatacata caggcctgcg
     2881 tagttctgaa ctatgtgccc ttcgatggcg tgatatcgac tttgttggaa aaacagcaca
     2941 tgtccagagc gcaagtgttg ttggtgttat caaagggacc aaaacaaaag ccggtactcg
     3001 taaagttgaa ctgacagaag aggcaatgtt ggcgctgata aatcagaagc catttacgtt
     3061 catgaaagat gctacggtct ttgaagatcc aaagaccaat aagccttggg caagtgctga
     3121 tgcaatcagg aaaaaagcat gggtgccaac attgcgaaaa gcaggtattc gctacagaaa
     3181 cccatatcag accaggcaca cttatgctac taagtacatt agccaaggag ctaatctctt
     3241 ctggctagca gggcagatgg ggcataaggg gcccgaaatg ttgtttcgtc attatgggcg
     3301 ttacttgagt gattatgaca accacagctc aacaacgctc acaagtaatt gcaaaaagta
     3361 gccttgagca aggtaaagca tgttattcaa agaagcctat ttcgttcaaa acgagaggtc
     3421 ctccacaaat ccacgcttga ataggtattc tttacaatta tgagcatacc ccttatagat
     3481 ttattccatt gaaaaatgcc atccccctga taaaacaaca gtattattgt ttcaaaaaaa
     3541 atgttataag taaattcact tacatgtaac atttaaattt ttgtttaaga a
//
LOCUS       DAZFXT010000240         3541 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.241, whole genome shotgun sequence.
ACCESSION   DAZFXT010000240 DAZFXT010000000
VERSION     DAZFXT010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.241"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            81..817
                     /locus_tag="ACHKW0_004910"
                     /pseudo
     CDS             81..817
                     /locus_tag="ACHKW0_004910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            996..1292
                     /locus_tag="ACHKW0_004911"
     CDS             996..1292
                     /locus_tag="ACHKW0_004911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7477566.1"
                     /translation="MPDQSGHCRTPSRILRMPELSQLLGISRSTIYEKMNPLSKYYDA
                     TFPRPVRLGSGSVGWRSSAIDEWLTLHTVPARSAVKGVNDEVRHTLSSDMTMSR"
     gene            1600..1716
                     /locus_tag="ACHKW0_004912"
     CDS             1600..1716
                     /locus_tag="ACHKW0_004912"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477567.1"
                     /translation="MSIACQNQVDVCSPGNLRGAVNIYTMVLEKGKRPGERY"
     gene            1935..3368
                     /locus_tag="ACHKW0_004913"
     CDS             1935..3368
                     /locus_tag="ACHKW0_004913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfjI family protein"
                     /protein_id="HFO7477568.1"
                     /translation="MCLLAPENPYPIYALPPLVRNAIIETQKNTQAPLAMVATSALTA
                     ISIACQNQIDVCRPGNLHGPVNLYSLILADSGERKTTVDKVFMKAFYLRDEALADEYA
                     KLVENYSTEKEIWEQKQKALESKFHKEIRAGKDYKATESELETHLNKPPVPPQIRRTI
                     FNETTIEGMLKYYSDSNRSFALVSSEGGVIFDSRAMSKLGIINTLWDGGSLFIDRKSS
                     PGINLKEPRLTISVMIQPDVYHKGFCTRKKEIVKTSGHHARFLMCQPTSTQGTRIITG
                     DNYSSQYQDLFEERINELIDESLAMSGERRCLHFSPQAARIWTDYYNDVESKLGGLGP
                     LRHCREYAAKNAEYMARLAGLIYHSSGEEGEISPYTAEMARELAIWYGNEYVRLSNPL
                     TFDNSALTVPVRLIPEELELFNWIKSYCIEKGILCMKKNDILQRGPNRFRKKDKINWL
                     LDLLYEQNRVVPVIEGKTLCVAPNFDL"
BASE COUNT         1086 a          692 c          789 g          974 t
ORIGIN      
        1 taacagatta tgtcagaagg ccccggtgat gccttctgac gatagattaa tgttgtattc
       61 tattggtatc tggagaccat atggcaacag ttacagtata ttctccccgc tgtaattcct
      121 ataaagtgta ccgttatgat cacagcacct cagatcatga gcgtttccag tgctaatcct
      181 gcaaacgtgt atttcaactg aattacatct acgaaacccg aaaaccaggc attaaagagc
      241 aaattgttga aatggttcat aacggagccg gggttcgcga tagctccaga acgctgaagg
      301 tggatatcaa caccgtcatt ctcactttaa aaaacgctca ccacgtaaag taaccatatc
      361 tccagccgcc agtgtcgatg tggcacttat ctgtgaactt gatgaacaat ggagttttgt
      421 cgaaaacaaa gctcgtcagc agtggcactg gtacgcgtat aagaccaaag ctgacggtgt
      481 gctggcttac acttttggtc ctcgcactga tgaaacatgc cgtgagttgc cggaattcct
      541 gaagccattc tccgcaggca tgataaccag agacaaccgg agcagttata ccagagaaat
      601 gccacaggat aagcatcttg tcggcaagat atttacccgg cgcattgagc gcaacaatct
      661 gacacttcgt actcacatca aacgacctgc ccggaaaacg atttgctttt tacgctccct
      721 ggaaattcac gaaaagccat tggttcattt atcgaaaaca catgtactac tgacaggagt
      781 cattaccagg gcctcttttg ctgttttttt gccatgaatg ataatgagaa tcgacagcga
      841 taagggaaaa gtaaaataac actaatgctg tcgttttcta tcatattgat ttttaattat
      901 ttattgtctt ttttctcaac ccattttttt acgcagacaa atttacccgg ggaatacaaa
      961 ctgtaacgac agttcaccac actatggggg ataaaatgcc agatcaatcc ggacactgca
     1021 ggactccttc acgcatctta cggatgcctg aactgagcca attgttaggt atatctcgtt
     1081 cgacaattta cgaaaaaatg aatccgttat cgaaatacta cgatgcaacc ttccccaggc
     1141 cggtacgtct tggtagtggt tctgtgggct ggcgttcatc agctattgac gaatggctta
     1201 ctttacatac tgtacctgcc agatcagcag tcaaaggtgt taatgacgaa gtgagacata
     1261 cgctttcttc agatatgacc atgagcagat aagtttttgt catcacgtga tcacctcagg
     1321 aggaaaaaga agcataagaa caatttagcc agaagtttaa ttgataaaca gaatttccag
     1381 cattcattgt tgaaagagca gctattgtcg ctattgtagc ggtaattgct gctataaccg
     1441 aaacgtatta cgaaaaagga agcatttatg ccttacatca aaccggatat gtcgtattca
     1501 gtatatgcat gaccatcaat gatagggaag gcaataatta aagctcaata caataaacaa
     1561 gtacctctga ctctggttgc aatgtcagta ttaacagcga tgtcaattgc ctgtcagaat
     1621 caggttgacg tatgcagtcc cggaaattta cgtggggccg ttaatattta cactatggtt
     1681 ctggagaaag ggaaaagacc gggggaaagg tattagtgaa ataatttccg gaggtattaa
     1741 gaatgagaaa tatgaatgat atttcgtggt gaataataca gtcttcactg aattaacctt
     1801 atcggtttat taaacatcag atctacaaat gctctgtatt tctcagagca tcaggtctga
     1861 atagcagcta ttttcgctat tgtagcggga tttgctgcta ttttactaac ccattaatga
     1921 aaagagaggc ttttatgtgc ttacttgcac cagaaaatcc ttatccgata tatgcactac
     1981 caccgctggt gagaaatgca ataattgaaa ctcaaaaaaa tacacaggca cctttggcta
     2041 tggtggcaac atccgcatta acagcgatct caattgcctg tcagaatcag attgacgtgt
     2101 gcagacctgg aaatttacat gggcctgtta atctttactc tctgattctg gctgattccg
     2161 gtgaaaggaa aacgaccgtg gataaagtgt ttatgaaagc attttatctc agggatgaag
     2221 ccctggcgga tgaatacgcg aaactggttg agaattacag tacagaaaag gaaatatggg
     2281 agcaaaaaca aaaagcgctg gaatcaaaat ttcataaaga gattcgtgcc ggtaaagatt
     2341 acaaggcaac agaatcagag cttgaaacgc atctgaataa acctcctgtt ccgccgcaga
     2401 tacgtcgaac aatttttaat gagacaacga tagagggaat gttaaaatat tactccgata
     2461 gcaatcgctc ttttgctctt gtatccagtg aagggggggt aatttttgac agccgggcca
     2521 tgagtaaact gggaattatt aacactttgt gggatggagg ttctcttttc atcgacagga
     2581 aatcatctcc cggaattaat ttgaaggagc caagactgac gatatcggtg atgattcagc
     2641 ctgatgttta ccacaaaggt ttttgtacgc gaaaaaaaga aattgtgaaa acgtcaggac
     2701 atcatgcaag gtttttgatg tgtcaaccaa catcaacgca ggggacaagg ataataaccg
     2761 gagataatta ttcatcacag tatcaggatt tgtttgaaga gaggatcaat gagcttattg
     2821 atgaaagtct ggcgatgagt ggtgaacgac gttgccttca cttttctcct caggctgcca
     2881 gaatctggac ggattattac aatgatgtgg aatcgaagct ggggggattg gggcctttaa
     2941 gacattgccg ggaatatgct gctaaaaatg cagagtatat ggcaagactg gcaggactta
     3001 tttaccattc gagcggtgaa gagggggaga tttcccctta cactgcagaa atggcgagag
     3061 aattagcaat atggtacggt aatgagtatg tgcggttgtc taatccgtta acttttgaca
     3121 actctgccct gaccgtacct gtgcgactca ttccagagga gcttgaactt ttcaactgga
     3181 taaaaagcta ttgtattgag aaggggatcc tctgtatgaa aaaaaatgac attttacagc
     3241 gtggtccgaa ccgtttccgg aaaaaggata aaatcaactg gttactggat ttactgtatg
     3301 aacaaaacag agttgtaccg gttattgagg gaaaaacgtt gtgtgtcgca cctaactttg
     3361 acctctgaac gacttaatcg ttgagtacat atagtctcct ctgctacttg cggaggagac
     3421 taatgtcttt accatgtcag cgttgtatta gaaaactctg gtacaggtca gtcagtaaat
     3481 ataactggtg taaccactat ttactattgg atagagtgtt actcaggata ttgtcggctt
     3541 t
//
LOCUS       DAZFXT010000241         3433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.242, whole genome shotgun sequence.
ACCESSION   DAZFXT010000241 DAZFXT010000000
VERSION     DAZFXT010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.242"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..217)
                     /locus_tag="ACHKW0_004914"
     CDS             complement(<1..217)
                     /locus_tag="ACHKW0_004914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477569.1"
                     /translation="MAQNSRLHNSDNSAVFASGHGRRSHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEVKKALSLDYRHWIISVR"
     gene            complement(287..547)
                     /locus_tag="ACHKW0_004915"
     CDS             complement(287..547)
                     /locus_tag="ACHKW0_004915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477570.1"
                     /translation="MMRIEELREIFSEDGLYTVRVENGAIVSHCRIKCLQSQQRKSGA
                     ALIHFVDGLVTDGFILRANEFVTSLPSLKEAGIKAGFSAFED"
     gene            complement(764..919)
                     /gene="hokD"
                     /locus_tag="ACHKW0_004916"
     CDS             complement(764..919)
                     /gene="hokD"
                     /locus_tag="ACHKW0_004916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7477571.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1330..2124
                     /locus_tag="ACHKW0_004917"
     CDS             1330..2124
                     /locus_tag="ACHKW0_004917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO7477572.1"
                     /translation="MDRCGNYLLKDGRSLGQGSFGEVFKVDVYNLTQTHMTTYARKYF
                     SPCPDFDKTAIKELTDLRQRFLVEIKTQCTLNRINYDSIAPIVLFNTNGDKPYFVMEL
                     AECNLYEAIRNGMNYAERKSAVTQILKGIITIHENNYIHRDLKPGNILYYADGKYKIS
                     DFGLVKDRDTLRAEIKTKFKPNHMGTDGYRAPEIHDSGLFSFQSDIFAAGKIITDIYP
                     TERSEKLKRLIAKCCAHWPEERYQSTRELLEDFLKVTGEVNHEEHN"
     gene            2108..2824
                     /locus_tag="ACHKW0_004918"
     CDS             2108..2824
                     /locus_tag="ACHKW0_004918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HFO7477573.1"
                     /translation="MKNIIEMACFSLKQPQKKENEDFYLPPSFDSDFNIVFAVADGVG
                     SSEHSMLASHAAIRGIRHTLGTSFFSIESAFHSAKKEIDNLDISTATTLTIVQIKKNE
                     VLIGHSGDCRVYFNKNNKLNQLTTDQTRYQELLDSGEHKLRNLRNHKERLSSVLTNAL
                     SNTTDLNFELMSFPISQLIYNGYLQLYAMTDGAYKHWDVRPRFSEKTMLSPTAFASSL
                     RKRIEKNIMDDYTFIGVKVT"
     gene            complement(3084..3257)
                     /locus_tag="ACHKW0_004919"
     CDS             complement(3084..3257)
                     /locus_tag="ACHKW0_004919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477574.1"
                     /translation="MRKFKIIIETGIAGGDFEDEFEVDDDATPDEIHDEAKDIFFNYC
                     NYSYHEIKDEEEE"
BASE COUNT         1012 a          736 c          664 g         1021 t
ORIGIN      
        1 gcctgacgga gattatccag tgacggtagt cgaggctaag ggctttttta acctcgcatc
       61 cgcgcctgcg gtaacactga atgagccatt cggcctgttc ttcagtgcat gggggatgct
      121 ggtaccagtc agatttgaat gcatgagaac gccgcccgtg cccgctggca aagacggctg
      181 aattatcaga attgtgtagt ctggaatttt gcgccatcgg ctttctccgg tggcacagtg
      241 ttactcagca ggggttcagc cctgcgctga attgtagatg aattcactaa tcttcaaaag
      301 cagaaaaacc agccttaatc ccagcttctt tcagagacgg caacgatgtg acaaattcat
      361 ttgcacgcaa aataaaacca tccgtcacaa gcccatccac aaaatgaatt aacgcagctc
      421 cactcttcct ttgttgagac tgtaaacatt taatacggca gtggctgaca atagcgccat
      481 tctcaacgcg cacagtatag aggccatctt cactaaaaat ttcacgtaat tcttcgattc
      541 tcatcaacag aatccttcca gataaatagc actcccctgt tcggggtcca tccctcttct
      601 ccctgcgcgc tacttaagta tttttgattc tattccggcg ccgtccggaa cttcaaacgc
      661 gttgaaaata aaaacaaaaa cccgccgaag cgggttaagt gcgggtgcgt tgaggatgcc
      721 tgacacatca gaggtggcga gggatttctc ccccgccagt ctcttactcc tcaggttcgt
      781 aagctgtgaa gacagcgacc tccgtctggc cggttcggat tcgtacctcg cagaggtctt
      841 tcctcgttac cagtgccgtc actatgacgg ttaaacagat gacgatcagg gcgattagca
      901 tcgccttttg ctgcttcata gcctgcttct ccttgccttt cggcacgtaa gaggctaacc
      961 tacgtgtgta gagcatagat atggcctcag attaatgtta agcgtcttgc cggacgcgta
     1021 atgttaactg gggcttttct ctatctgcct tttggtgttc atgcctgaga cagatagcct
     1081 caagcacccg cagcaatttt acttaactcc ccttttccca caaatcgttt ttatccctat
     1141 tggtaatgtt ctcccgatgt gggattccca cattggaacg aattcaatca gttaaagact
     1201 catcagaatc tcacctttct aagtgcccac aggtgaggca ttgcaataaa aatagcaaac
     1261 gtgtataaaa ggaggtaatg catgtttttc cagtcttatc aatccattac tgagagttaa
     1321 gggggcgccg tggatcggtg cggtaattat ttacttaaag atggtcgcag tttaggacaa
     1381 gggagttttg gcgaagtatt taaggttgat gtctataact taacccagac ccacatgacc
     1441 acatatgctc gcaaatactt ttcaccttgc ccggactttg acaagaccgc tataaaagag
     1501 ttgactgatt taagacagcg ttttttagtt gagatcaaaa cacaatgcac tctcaataga
     1561 ataaactatg attcaatagc gccaatagtt ttatttaata caaatggtga taaaccttat
     1621 tttgttatgg aattggcaga gtgtaactta tatgaagcca tccgcaatgg tatgaactac
     1681 gcggagagga agtctgctgt tactcagatt ttaaaaggaa ttatcaccat acatgaaaac
     1741 aactatatac atcgtgattt aaaaccagga aacatacttt attatgccga tggaaagtat
     1801 aaaatatctg attttggtct agtaaaagac agagatacct tacgggccga aatcaaaaca
     1861 aaatttaagc ctaatcacat gggcacagat ggatatcgag cgcccgaaat acatgatagc
     1921 ggtctttttt cttttcaaag tgacatattt gcagcaggta aaattatcac tgatatatat
     1981 cccacagaaa gaagtgaaaa attaaaaaga ttgattgcaa aatgttgcgc acattggcct
     2041 gaagaacgat atcaaagcac acgggagtta ctggaagatt ttttaaaagt gacgggggag
     2101 gtaaatcatg aagaacataa ttgaaatggc atgttttagc ctaaaacaac cccaaaaaaa
     2161 agaaaatgaa gacttttatt taccgccatc ttttgatagc gattttaaca ttgtctttgc
     2221 agtagctgat ggggttggtt cctctgaaca ttcaatgctt gcatctcatg cagccattcg
     2281 tggcattaga cacacgctag gcacttcctt tttctcaatt gaaagtgcat ttcattcagc
     2341 taaaaaagaa attgacaatt tagatattag cactgccaca accttaacaa ttgttcagat
     2401 caaaaaaaat gaagtgttaa ttggtcattc cggtgattgc cgagtatact tcaataaaaa
     2461 caataaacta aatcagttaa ccactgatca aacgagatat caagaactcc ttgattctgg
     2521 tgaacataaa ttacgtaatt taagaaatca taaagaacgg ctatcttcag tcctaacgaa
     2581 tgctttatca aatacaacgg atttgaattt tgagttaatg tcttttccga ttagccagct
     2641 aatttataat ggctatcttc aactctatgc aatgactgat ggtgcctata agcattggga
     2701 tgtcagacca aggttttctg agaagacaat gctctcacca acagcttttg cgagtagttt
     2761 aagaaaaagg attgagaaaa acataatgga tgattacacc tttataggcg taaaagtaac
     2821 ctaattttag atatccaaga tatttactct aatttaacca tagtattccc aacctctttt
     2881 tattgggggt tggattcatt aaagtcacca ttttctattt ctttcccgtt agaggatata
     2941 ttatttccag ataaagaaag caacttctta tgcaacttca tatcacgcca tgcttccacc
     3001 gcggtaactc tacatagtcc attaacagtt catgccattc ttcaaaattg gcgttatatt
     3061 tagttgaagc aaaatcagcc attttattct tcctcttcgt cttttatttc gtgatatgag
     3121 taattgcagt agttaaagaa aatatctttt gcttcgtcat gtatttcatc aggcgtcgca
     3181 tcatcatcca cttcgaattc atcctcgaaa tctccaccgg ctattcccgt ttcaataatt
     3241 attttaaact ttcgcattta actaccgccc tttcgggcgg cctcctgatg ttcttagggt
     3301 gcagaaatcc ctccggttaa ggattaaatt tttaacagag ctaaatttaa ttattcagtt
     3361 ctggattttg tcgccctgcg tatccgcgct ttcgcgttac gctcaatctg aattagcttt
     3421 tctatatttt ttc
//
LOCUS       DAZFXT010000242         3426 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.243, whole genome shotgun sequence.
ACCESSION   DAZFXT010000242 DAZFXT010000000
VERSION     DAZFXT010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3426)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3426)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3426
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.243"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..230)
                     /locus_tag="ACHKW0_004920"
     CDS             complement(<1..230)
                     /locus_tag="ACHKW0_004920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO7477575.1"
                     /translation="MQQLSFLPGEMTPGERSLIQRALKTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETL"
     gene            complement(246..725)
                     /locus_tag="ACHKW0_004921"
     CDS             complement(246..725)
                     /locus_tag="ACHKW0_004921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7477576.1"
                     /translation="MKTVSQNTPTIYSATTPENNPPQLVASLVPDEQRISFWPQHFGL
                     IPLWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMN
                     GNRAEMSPEAAGIAACLMTYSHHACRTENYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            complement(807..1628)
                     /locus_tag="ACHKW0_004922"
     CDS             complement(807..1628)
                     /locus_tag="ACHKW0_004922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7477577.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELIRHVPSIFGEDRHTSRSER
                     YAYIPTITVLENLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEII
                     LLNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGKVRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLW
                     SAYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETMLESLR"
     gene            complement(1849..2259)
                     /locus_tag="ACHKW0_004923"
     CDS             complement(1849..2259)
                     /locus_tag="ACHKW0_004923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477578.1"
                     /translation="MKTFIKTLLVAVTILFSVFATAKQVKLPNNIKYVNTTEAFSCTE
                     IDGMNCQTKNQFNYKDNSYVFVLERGGAWCYDYTVSVVNLKTGKAQMIEYGDNQLCSG
                     SNKPFFEIKNGVPTVGVIDTSGKPVVVAQDKLKI"
     gene            complement(2275..2958)
                     /locus_tag="ACHKW0_004924"
     CDS             complement(2275..2958)
                     /locus_tag="ACHKW0_004924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477579.1"
                     /translation="MKLPVKLLMSLISLVSVIARAGEYKNYSRDEIKYWRYTSYKGGK
                     LPEGFTDEKFSSAIYNGRIFTMKRLHTLMLFLAVLFTGFNFNAEAATVKQALSCNPEA
                     WAEQPGACPSTYELYEGDATYKAAIDKALKPVGLSGMFGKGGYMDGPGGGITPVNING
                     TVWFQGDGCKANTCGWDFIVTLYNPKTHEVVGYRYFGLDDPAYLVWFGEIGVHEFAYL
                     VKNYVAAVN"
     gene            complement(3094..>3426)
                     /locus_tag="ACHKW0_004925"
     CDS             complement(3094..>3426)
                     /locus_tag="ACHKW0_004925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477580.1"
                     /translation="NMVIVTHLSDGSLWDRQAFPDTTILEIRPRKRLKYAGDGGNSGG
                     LLSFTSAHTDAWRQQGYEDTMLAMEHIRKPLAARQALTRSEAVLQKSLDITEEADLAL
                     RNAMARIK"
BASE COUNT          807 a          870 c          846 g          903 t
ORIGIN      
        1 aagagggttt caccggcaat cagttgattc tggttattca gatacagcac ccggaattct
       61 tcacgctcca gtcccgccat gttcagaatc agccattccc gtgctgcacg ggtggaggtg
      121 aaggccacgc cgggttcatg aagatggcgg tccagggttt tcagggcccg ttgaatgagg
      181 ctgcgctcgc cgggcgtcat ctctccgggc agaaaggaaa gttgttgcat tgtgcttctc
      241 tccattcagt cgatgatgcg cataatggcg ctgcattccg gatgctgcag ggcgtaatcc
      301 cgcaaccggt aataatggac cgtcatggca taattctccg tacgacaggc atgatgactg
      361 tacgtcatca gacaggcggc aataccggcg gcttccgggc tcatttctgc gcggttaccg
      421 ttcatggcat tgaacagtac ccatgtttcg tcatcatcgt catccggttc gggtgccata
      481 aatgccccgc cgttgttcag ggtgtacaga ttccagatac caccgcagta gtcttcgcac
      541 agacggtcca tccagccgaa gacacggggc tccagggtca cccacagtgg aatgaggcca
      601 aaatgctgcg gccagaagct gatgcgctgt tcatcaggga cgaggctggc aaccaactga
      661 ggcggattat tctctggtgt tgtagctgaa taaattgtgg gggtattctg agaaacggtt
      721 ttcatgagat aactccttaa ataacaaata aatgtgtgtt tttcaggaac gaaagctgaa
      781 tgattccctc actcagggaa acggcatcag cgcaggctct ccagcatcgt ttctgccatc
      841 acccacaatg cgcgattgag cttaatgtcg gtatcaatgc tgtgaatggc ccgggtatgg
      901 atacgttttc cttttgcact gcgaccggaa atcccgcctt tcagcatatt ctcctggatg
      961 gtctgatatg cgctccacag gtccttaccg taatcctccc ggcgtcgcgg cgtcagaatg
     1021 tcggcggtgg tgacgggctg atgttcgtca ccataacggt aagtcagtgc cgcctgtgcc
     1081 agcgcctggc gtgccggtgg cggcaggacc agcgactgca tggcatcacg cttctcctct
     1141 atccggtcaa acacgcccac cacctcgtaa gccccttcga taactttctc cactacattt
     1201 ccccggtgtg gaacacgcac tttccccaga gactggccac agacgcaccc gttctggcag
     1261 acgaacctga agtaacccgg cagcatctgg tagctggagg taccgtcatg agagttgagc
     1321 agaataattt cagggacatg ttctccgttt atctctccgg cccgccgcag acgcagcatg
     1381 tgttttgtgt atccccggcg gcccgggtcg cgcacacggg tctggcaggc gaagaacggc
     1441 tgaaagcctt cccgctgcag attttccagg acggtaatgg tgggaatgta cgcataccgt
     1501 tcactgcggg aggtgtgccg gtcttcccca aaaatactgg gtacatggcg tatcagttct
     1561 tcatgtgtca gcggacggtc acggcgtatc tggtttgcag cataaccaaa acgacttgct
     1621 aatcgcatac tttgctcctt atcggtggtt aagatttact ggtgcaataa atgaaaaaag
     1681 ccgcgtctcc cggagaagac gcggcctgac gggtgaaatg aataaggttt attgtctgag
     1741 aggcctttca ctggcgagcc acgcattaag cggctttccg gtaacaatgg ctggggctgt
     1801 atttaccttt gatattaata atgtgcagag aaacgcatgg tttactcctt agattttaag
     1861 tttgtcctgg gctacaacga caggttttcc ggatgtgtcg atgactccta ccgtcggtac
     1921 gccatttttg atttcgaaga acggtttgtt gctacctgag cacagttgat tgtccccgta
     1981 ttcaatcatt tgtgctttcc cggttttcag gttaactacc gagacagtgt agtcgtagca
     2041 ccaggcacca ccacgttcaa gcacgaaaac atagctgtta tctttatagt tgaactgatt
     2101 ctttgtctgg caattcatac cgtcaatttc ggtacaggaa aacgcttctg tagtattaac
     2161 gtatttgatg ttatttggca gttttacttg tttcgcagta gcgaagacag agaacagaat
     2221 agttacagca acgagtaaag ttttgataaa agttttcatg atagactcct taaattagtt
     2281 aaccgcagct acgtagtttt tcaccagata cgcgaattca tgcacgccaa tttcaccgaa
     2341 ccaaaccagg taggccgggt catctaaacc aaagtaacgg tagccaacaa cttcatgggt
     2401 ttttgggtta tagagtgtta cgataaagtc ccagccgcag gtattggctt tgcaaccgtc
     2461 gccctggaac cagactgtac cgttaatgtt tactggcgtg atacctccac cagggccatc
     2521 catatagccg cctttaccga acatgccgct cagtccgacc ggttttaatg ctttgtcgat
     2581 agcggctttg taggtagcgt caccttcgta caactcgtac gttgatggac acgctccagg
     2641 ttgttcagcc caggcttccg ggttacaact aagagcttgt tttacggtag ccgcttctgc
     2701 gttaaagtta aagcccgtga acagaaccgc cagaaataac ataagggtat gtaaacgttt
     2761 cattgtaaat attcttccgt tgtaaatggc gctggaaaat ttctcatcag tgaaaccttc
     2821 cggaagtttc ccccccttgt atgatgtata tcgccagtat tttatttcat cccggctgta
     2881 atttttatat tccccggcac gtgcaataac gctgaccaga gatataaggc tcattaataa
     2941 ctttacaggt aatttcataa ttaaatttcc agtgagtgaa ttgacaggtg aggggtaaaa
     3001 ctttctctca ggtaatatat atctgaaaat attttcaata acaccagaca ggaaaaacac
     3061 caccacaggc ggtggcgttt ctttggggcg tctttattta atccgggcca tcgcgtttct
     3121 cagtgccaaa tctgcctctt ccgttatatc caggcttttc tgcaataccg cctctgaccg
     3181 ggtcagtgcc tgacgtgctg ccagcggttt ccggatatgc tccatcgcca gcatcgtgtc
     3241 ttcatagccc tgctgacgcc aggcgtcggt atgtgccgat gtaaaactga gcagaccgcc
     3301 gctgttgcca ccatcacctg catatttcag ccttttccgg ggacggatct caaggattgt
     3361 ggtgtccgga aaagcctggc gatcccataa agaaccgtca ctcagatgcg tcacaatcac
     3421 catatt
//
LOCUS       DAZFXT010000243         3397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.244, whole genome shotgun sequence.
ACCESSION   DAZFXT010000243 DAZFXT010000000
VERSION     DAZFXT010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.244"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            6..200
                     /gene="ylcI"
                     /locus_tag="ACHKW0_004926"
     CDS             6..200
                     /gene="ylcI"
                     /locus_tag="ACHKW0_004926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7477581.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALDQKGSGNFSAWVIEA
                     CRRRLTSEKIAYTSIKSDEE"
     gene            complement(365..571)
                     /gene="ybcW"
                     /locus_tag="ACHKW0_004927"
     CDS             complement(365..571)
                     /gene="ybcW"
                     /locus_tag="ACHKW0_004927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbcW"
                     /protein_id="HFO7477582.1"
                     /translation="MNKEQSADDPSVDLIRVKNMLNSTISMSYPDVVIACIEHKVSLE
                     AFRAIEAALVKHDNNMKDYSLVVD"
     gene            857..1267
                     /gene="ybcV"
                     /locus_tag="ACHKW0_004928"
     CDS             857..1267
                     /gene="ybcV"
                     /locus_tag="ACHKW0_004928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO7477583.1"
                     /translation="MAQVAIFKEIFDQVRKDLNCELFYSELKRHNVSHYIYYLATDNI
                     HIVLENDNTVLIKGLKKVVNVKFSRNTHLIETSFDRLKSREITFQQYRENLAKAGVFR
                     WVTNIHEHKRYYYTFDNSLLFTESIQNTTQIFPR"
     gene            1558..1851
                     /gene="bor"
                     /locus_tag="ACHKW0_004929"
     CDS             1558..1851
                     /gene="bor"
                     /locus_tag="ACHKW0_004929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serum resistance lipoprotein Bor"
                     /protein_id="HFO7477584.1"
                     /translation="MKKMLLATALALLITGCAQQTFTVQNKQTAVAPKETITHHFFVS
                     GIGQKKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSK"
     gene            complement(1942..2124)
                     /gene="rzoD"
                     /locus_tag="ACHKW0_004930"
     CDS             complement(1942..2124)
                     /gene="rzoD"
                     /locus_tag="ACHKW0_004930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HFO7477585.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPRPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(2341..2838)
                     /gene="rrrD"
                     /locus_tag="ACHKW0_004931"
     CDS             complement(2341..2838)
                     /gene="rrrD"
                     /locus_tag="ACHKW0_004931"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO7477586.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTKAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(2838..3053)
                     /gene="essD"
                     /locus_tag="ACHKW0_004932"
     CDS             complement(2838..3053)
                     /gene="essD"
                     /locus_tag="ACHKW0_004932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HFO7477587.1"
                     /translation="MKFMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         1008 a          774 c          670 g          945 t
ORIGIN      
        1 acctcatgtc aacgaagaac agaacccgca gaacaacaac ccgcaacatc cgctttccta
       61 accaaatgat tgaacaaatt aacatcgctc ttgatcaaaa agggtccggg aatttctcag
      121 cctgggtcat tgaagcctgc cgtcggaggc taacgtcaga aaagatagca tatacatcaa
      181 ttaaaagtga tgaagaatga acatcccgcg ttcttccctc cgaacaggac gatattgtaa
      241 attcacttaa ttacgagggc attgcagtaa ttgagttgca gttttaccac tttcctgaca
      301 gtgacagact gcgtgttggc tctgtcacag gttaagtagt ttgaatgatt agcagttatg
      361 gtgatcagtc aaccaccagg gaataatcct tcatattatt atcgtgcttc accaacgctg
      421 cctcaattgc cctgaatgct tccagagaca ccttatgttc tatacatgca attacaacat
      481 cagggtaact catagaaatg gtgctattaa gcatattttt tacacgaatc agatccacgg
      541 agggatcatc agcagattgt tctttattca ttttgtcgct ccatgcgctt gctcttcatc
      601 tagcggttaa aatattactt caaatctttc tgtatgaaga tttgagcacg ttggccttac
      661 atacatctgt cggttgtatt tccctccaga atgccagcag gaccgcactt tgttacacaa
      721 ccaatactat taattgaaaa cattcctaat atttgacata aatcatcaac aaaacacaaa
      781 gaggtcagac cagattgaaa cgataaaaac gataatgcaa actacgcgcc ctcgtatcac
      841 atggaaggtt ttaccaatgg ctcaggttgc catttttaaa gaaatattcg atcaagtgcg
      901 aaaagattta aactgtgaat tgttttattc tgaactaaaa cgtcacaatg tctcacatta
      961 tatttactat ctagccacag ataatattca cattgtgtta gaaaacgata acactgtgtt
     1021 aataaaagga cttaaaaagg ttgtaaatgt taaattctca agaaatacgc atcttataga
     1081 aacgtccttt gataggttga aatcaagaga aatcacattt cagcaataca gggaaaatct
     1141 tgctaaagca ggagttttcc gatgggttac aaatatccac gaacataaaa gatattacta
     1201 tacctttgat aattcattac tatttactga gagcattcag aacactacac aaatctttcc
     1261 acgctaaatc ataacgtccg gtttcttccg tgtcagcacc ggggcgttgg cataatgcaa
     1321 tacgtgtacg cgctaaaccc tgtgtgcatc gtttttaatt attcccggac actcccgcag
     1381 agaagttccc cgtcagggct gtggacatag ttaatccggg aatacaatga cgattcatcg
     1441 cacctgacat acattaataa atattaacaa tatgaaattt caactcattg tttagggttt
     1501 gtttaatttt ctacacatac gattctgcga acttcaaaaa gcatcgggaa taacaccatg
     1561 aaaaaaatgc tactcgctac tgcgctggcc ctgcttatta caggatgtgc tcaacagacg
     1621 tttactgttc aaaacaaaca gacagcagta gcaccaaagg aaaccatcac ccatcatttc
     1681 ttcgtttctg gaattgggca gaagaaaact gtcgatgcag ccaaaatttg tggcggcgca
     1741 gaaaatgttg ttaaaacaga aacccagcaa acattcgtaa atggattgct cggttttatt
     1801 actttaggca tttatactcc gctggaagcg cgtgtgtatt gctcaaaata attgcatgag
     1861 ttgcccatcg atatggtcag ctctatctgc actgctcatt aatatacttc tgggttcctt
     1921 ccagttgttt ttgcatagtg atcagcctct ctctgagggt gaaataatcc cgttcagcgg
     1981 tgtctgccag tcggggggag gctgcattat ccacgccgga ggccgtggtg gcttcacgca
     2041 ctgactgaca gactgctttg atgtgcaacc gacgacgacc agcggcaaca tcatcacgca
     2101 gagcatcatt ttcagctttc gcatcagcta actccttcgt gtattttgca tcgagcgcag
     2161 caacatcacg ctgacgcatc tgcatgtcag taattgccgc gttcgctagc ttcagttctc
     2221 tggcattttt gtcgcgctgg gctttgtagg cgattgcgtt atcacggtaa tgattgaccg
     2281 cccatgacag gctgacgatg atgcagataa tcagagcgga tataatcgcg gttactctgc
     2341 tcactgttgc ccccacaaac agacttcacg ctcaatctca cgacgagtca tcaggccttt
     2401 ccattgctta ccgccagcgt atgtccagcg acgcagctga tcacatgcgc ctttgatatc
     2461 gccctggttt attttgcgaa gaagcgtcga tgttctaaaa ttgccagcac ccacattgta
     2521 aacgaatgag taaagagcgc cgcgcgttgt ttccggtata tcaactttga tatacgggtt
     2581 aatttgtctg gcgacagtgg caaggtcttt attcaagagt gctttgcatt ctgctttggt
     2641 atacgtttta ccgagcatga tgtcttttcc ggtgtgtccg tgacatacag tccatacacc
     2701 aacaatatct ttgtatggta tgtagctgac accttccaga ccatcgttac cacttgggcc
     2761 agtgattaac actgatgcta tagcaattgc tccgccacca atagcagcag caacggcttt
     2821 tcgtaatgat ggaggcatta ttcacctctc gcagccttgc gcttatcttc tttaatcttg
     2881 aaataaaggt ttgtcaggta cgtcagcagg ccaaatacca ggctacccag cacacctatt
     2941 gctgcccact gtgagggagt gactttatcg agcagctgta aaaaccagta accggcacta
     3001 cctgctgagg tgccataggc gactccagtt gttaacttat ccatgaattt cataacccca
     3061 cctcgcagac aaagcgggtg taaattgagg gaatacaacg tatcgcaaaa aagcagaaac
     3121 gtaactgact ccgagtcagt gaataactca ggtattgagt tatcagctaa tatcgagact
     3181 caaaaaatgg aaaaaccagc tcgacggcgg gtttaagctg tgtgacgaag taaccactct
     3241 taacagcata accaattttt tacgtacgta aaccactgaa tgatatttat gagaatgcta
     3301 ccgagtgttc aaaacatcac cacaaataca taagaaaacc tcaacaaata accaataaat
     3361 aatttctagt gttattttta gccggtttaa attaaac
//
LOCUS       DAZFXT010000244         3387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.245, whole genome shotgun sequence.
ACCESSION   DAZFXT010000244 DAZFXT010000000
VERSION     DAZFXT010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.245"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            121..1086
                     /gene="dusB"
                     /locus_tag="ACHKW0_004933"
     CDS             121..1086
                     /gene="dusB"
                     /locus_tag="ACHKW0_004933"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HFO7477588.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            1112..1408
                     /gene="fis"
                     /locus_tag="ACHKW0_004934"
     CDS             1112..1408
                     /gene="fis"
                     /locus_tag="ACHKW0_004934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HFO7477589.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            1494..2378
                     /gene="yhdJ"
                     /locus_tag="ACHKW0_004935"
     CDS             1494..2378
                     /gene="yhdJ"
                     /locus_tag="ACHKW0_004935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HFO7477590.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            2462..2641
                     /locus_tag="ACHKW0_004936"
     CDS             2462..2641
                     /locus_tag="ACHKW0_004936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HFO7477591.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRI"
     gene            complement(2644..3306)
                     /gene="acrS"
                     /locus_tag="ACHKW0_004937"
     CDS             complement(2644..3306)
                     /gene="acrS"
                     /locus_tag="ACHKW0_004937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HFO7477592.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
BASE COUNT          929 a          781 c          803 g          874 t
ORIGIN      
        1 attttaaatg caattctttg atccatctca gaggattggt caaagtttgg cctttcatct
       61 cgtgcaaaaa atgcgtaata tacgccgcct tgcagtcaca gtatggtcat ttcttaactc
      121 atgcgcatcg gacaatatca gctcagaaat cgcctgatcg cagcgcccat ggctggcatt
      181 acagacagac cttttcggac gttgtgctac gagatgggag ccggattgac agtatccgag
      241 atgatgtctt ctaacccaca ggtttgggaa agcgacaaat ctcgtttacg gatggtgcac
      301 attgatgaac ccggtattcg caccgtgcaa attgctggta gcgatccgaa agaaatggca
      361 gatgcagcac gtattaacgt ggaaagcggt gcccagatta ttgatatcaa tatgggttgc
      421 ccggctaaaa aagtgaatcg caagctcgca ggttcagccc tcttgcagta cccggatgtc
      481 gttaaatcga tccttaccga ggtcgtcaat gcagtggacg ttcctgttac cctgaagatt
      541 cgcaccggct gggcaccgga acaccgtaac tgcgaagaga ttgcccaact ggctgaagac
      601 tgtggcattc aggctctgac cattcatggc cgtacacgcg cctgtttgtt caatggagaa
      661 gctgagtacg acagtattcg ggcagttaag cagaaagttt ccattccggt tatcgcgaat
      721 ggcgacatta ctgacccgct taaagccaga gctgtgctcg actatacagg ggcggatgcc
      781 ctgatgatag gccgcgcagc tcagggaaga ccctggatct ttcgggaaat ccagcattat
      841 ctggacactg gggagttgct gcccccgctg cctttggcag aggttaagcg cttgctttgc
      901 gcgcacgttc gggaactgca tgacttttat ggtccggcaa aagggtaccg aattgcacgt
      961 aaacacgttt cctggtatct ccaggaacac gctccaaatg accagtttcg gcgcacattc
     1021 aacgccattg aggatgccag cgaacagctg gaggcgttgg aggcatactt cgaaaatttt
     1081 gcgtaaacag aaataaagag ctgacagaac tatgttcgaa caacgcgtaa attctgacgt
     1141 actgaccgtt tctaccgtta actctcagga tcaggtaacc caaaaacccc tgcgtgactc
     1201 ggttaaacag gcactgaaga actattttgc tcaactgaat ggtcaggatg tgaatgacct
     1261 ctatgagctg gtactggctg aagtagaaca gcccctgttg gacatggtga tgcaatacac
     1321 ccgtggtaac cagacccgtg ctgcgctgat gatgggcatc aaccgtggta cgctgcgtaa
     1381 aaaattgaaa aaatacggca tgaactaatt caggttagct aaatgcttga ttaaaaaggc
     1441 gctactcggc atggggaagc gcctttttta taggtgtcac aaagggagtg accatgagaa
     1501 caggatgtga accgacccgg tttggtaatg aagctaagac cattattcac ggtgatgcgc
     1561 ttgccgaact taaaaagatc cccgccgaaa gtgtcgatct gatctttgcc gacccaccat
     1621 ataacatcgg taaaaatttt gatggtctga tcgaagcctg gaaagaagat ctgtttatcg
     1681 actggctgtt tgaagtgatt gcagagtgcc accgcgttct gaaaaagcag ggcagcatgt
     1741 acatcatgaa cagcacggaa aacatgccct ttatcgatct ccagtgccgc aagcttttta
     1801 ccatcaaaag tcgcatcgtc tggtcatatg acagttctgg agtacaggcg aaaaaacact
     1861 acggctccat gtacgaaccc atcctgatga tggtgaaaga cgcaaagaac tacacattca
     1921 acggtgatgc tattctggta gaagccaaaa ccggatcgca gcgcgcgttg atcgattatc
     1981 gcaaaaatcc tccacagcca tacaatcatc aaaaagtacc gggtaacgtc tgggattttc
     2041 cgcgcgtgcg ttatttgatg gatgaatatg agaaccaccc gacgcaaaaa ccggaagcct
     2101 tactgaaacg cattattctc gcctcttcca acccaggcga tatcgttctc gacccgtttg
     2161 ccggtagctt tactaccggt gccgtagccg tctccagcgg gcgaaaattc attggtattg
     2221 agatcaacag cgagtacatc aaaatggggc ttcgacggct ggatgtcgcg tcgcattact
     2281 ctgcggaaga actggcgaaa gtgaaaaaaa gaaagaccgg caacctgtca aaacgaagcc
     2341 ggttaagcga agttgacccc gatctcattg caaagtaaag gaagtgtaag cctggttttc
     2401 agattattca tttcgtgtat atttcctgcc agacttggtt aaacatacac aggctcaggt
     2461 aatgattcgc aagtattggt ggctcgtcgt tttcgctgtc ttcgtttttc tgtttgatac
     2521 tttactgatg cagtggattg aactgctggc aacagaaaca gacaaatgcc gcaatatgaa
     2581 ctcagttaat ccactaaaac tggttaactg tgacgaactg aattttcagg acagaatatg
     2641 aatttacatg acacttaatt cattcgtttg atgaattaat ttcgttatgt tttcatctgg
     2701 catgaacatt cttaatacgt tatcgaccag agcgggggct tgtttataaa gatcataacc
     2761 cgccatattc attaaccagt tttgaacaat tccgctgaag gcaccattaa taataatcat
     2821 cacaacatct aaatcgaggt tatttgctac acaaccttgt tgctgacacg cctgcaatac
     2881 ttcgcggaga gtctgcggat tgaagcccat cttttcgcgt atcactccct cggccagcat
     2941 ctcatcatta aattcacatt tgtgatataa gattttcagc aacgcctgct ggcggggaat
     3001 tttggcaata tattgcaagc cgacaatcaa tttttcacgc aattgttgaa acgggtcatg
     3061 ctctaatcca gccgtcaagt gttcctggat taactcccgc aatgaaggct gttgcaacca
     3121 catctcatta aacagttgag tcttgttttc gaagtgccag tagatagcgc cacgcgtaac
     3181 gttagcagcg tcggcaatgt cgttgagtgt cgtcttgctt acgccatgct gcgcaaactg
     3241 ggcgatggca gtttcaatca gttcttgccg ggtcttcaga gcttcggctt tggttctttt
     3301 tgccatgatt aattattcag gaaataaata tattcgacac agagtgagaa aatagcgaag
     3361 gttaatctat cacctaatgt gtattta
//
LOCUS       DAZFXT010000245         3240 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.246, whole genome shotgun sequence.
ACCESSION   DAZFXT010000245 DAZFXT010000000
VERSION     DAZFXT010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3240
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.246"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHKW0_004938"
     CDS             complement(90..617)
                     /gene="mobB"
                     /locus_tag="ACHKW0_004938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HFO7477593.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHKW0_004939"
     CDS             complement(599..1183)
                     /gene="mobA"
                     /locus_tag="ACHKW0_004939"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HFO7477594.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            1253..1522
                     /locus_tag="ACHKW0_004940"
     CDS             1253..1522
                     /locus_tag="ACHKW0_004940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HFO7477595.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1599..2585
                     /gene="srkA"
                     /locus_tag="ACHKW0_004941"
     CDS             1599..2585
                     /gene="srkA"
                     /locus_tag="ACHKW0_004941"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HFO7477596.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQWMQLETIIEDYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2602..3228
                     /gene="dsbA"
                     /locus_tag="ACHKW0_004942"
     CDS             2602..3228
                     /gene="dsbA"
                     /locus_tag="ACHKW0_004942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HFO7477597.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          819 a          775 c          795 g          851 t
ORIGIN      
        1 aggcccagtc ggaagactgg gcctttttgc gctggtgcag aaaaaggtcg gatgcgacac
       61 tggctgcgtc tgatccagcc tactcttgct tatccgttct gcttttgcat ccactccacc
      121 acaaaatcag ccagcccctc aacatcatta atatccagta atgcgacatc aagattaagc
      181 ggcacatcac tggctacagc aataacatgc ctgtctatca ctaattcttc aggtcgatgt
      241 ccggctccat cgcgaaacag cacaatcttt gcgatctctt catgcttaaa cccttcgacc
      301 agaatcaaat ccagctttga ggtatccatt cgacttgcga gaaactgtag atccagctct
      361 tcttcgtctg gtgtttccgt catcaaggcc catcgttgct ggctggcaac gatggtttgt
      421 gccgcgccag ccttgcgcag ctcatagcta tctttgcctg gcttatcaac atccatatca
      481 tggtgcgtat gcttaatcag ccctggacgg atccctctgg cgcataatgc cgggatcagt
      541 tgtttcaaaa gcgtagtttt tccggtacca ctccacgcgg caaaggcgag taacggtatc
      601 atcgtttttc ctgccatcgg gcaagctcct ctggcgtatt cacgttaata aatgcatctt
      661 tattatcgct gaaatcaacc gcatgaccgc cagccagacg cataaatgcc attacccggc
      721 gttctcctgc ttgcagatat tccagtaata aaggctcaat tgcgcggttt accagagcaa
      781 tagtcgggtg atcgcgttca ccgtcgtgga cccacacgac aggcgcatct ttgcgctgat
      841 gattaagccg ggctgctaaa tcctcgggaa tataaggagt atcgcacgga caaaacaaaa
      901 accactcgcc tgcttcctgc tgcattactg aaagcattcc tgccagaggg cctgggtaat
      961 ccgccagtga atcttcaatc actttcagac cgcttgcctg atagatttcc tgatggcgat
     1021 tggcattaac cacgacgtga gagagctgcg tcataagcgc gtcagcgaca tgttgccata
     1081 atggtttgcc gtttaattca agcaatcctt tatctacgcc gcccattcgt ctggctttac
     1141 cgcctgccag cacaacgcct gttatcgtcg tcatcagatt caccgatatc gcctctttta
     1201 ttgtgggatt gaccctgcta acgtgtctgt ctcaagagta aggagcatta ctatgaaatg
     1261 taaacgtctg aatgaagtta ttgaactcct ccagccagcc tggcaaaaag agccagacct
     1321 taacctgctg caatttttgc agaaactggc gaaagagtca ggttttgacg gcgaactggc
     1381 ggatttgacg gatgacattc tgatctatca tctgaaaatg cgtgattccg cgaaagatgc
     1441 ggtgatcccg ggtttgcaga aagattatga agaagacttc aaaacggcgc tgttacgcgc
     1501 tcgcggcgta attaaagagt aaaagcttgt aagcggcgcc accaaaatca tcgtgaaatg
     1561 atatccttca tcattcgtaa tgttttccgg atgatgggat gaacaacagc gcttttactt
     1621 tccagacact acacccggat accatcatgg atgctctatt tgagcaaggg atccgggtgg
     1681 attccggtct taccccgctt aacagctatg aaaaccgtgt ctatcaattt caggacgaag
     1741 atcgtcgacg ttttgtcgtc aaattttatc gccctgaacg ttggacagcc gatcaaatcc
     1801 tcgaagaaca tcaatttgcg ttgcagctgg taaatgatga agttccggtc gcagcacctg
     1861 tggcctttaa cggtcagact ttattgaatc atcagggatt ttatttcgct gtttttccaa
     1921 gcgtcggtgg tcgccagttc gaagctgata atatcgatca gatggaagcg gttgggcgtt
     1981 atttagggcg tatgcaccag acggggcgca aacagctttt tatccatcgc ccgaccatcg
     2041 gtctgaatga atatctcatt gagcctcgca agctgtttga ggacgctaca ctgatacctt
     2101 ccgggttgaa agcggcattc ctgaaagcga cagatgagct gatcgccgcc gttacagcac
     2161 actggcggga agacttcacc gttctgcggc tacatggaga ctgccacgcc gggaatattc
     2221 tctggcgcga tggtccaatg tttgttgatc tggatgatgc acgtaatggt ccagctattc
     2281 aggatttgtg gatgttgctc aatggcgata aagccgagca gtggatgcaa ctggaaacta
     2341 ttattgaaga ttatgaagaa tttagcgagt tcgacaccgc tgaaatcgga ctgattgaac
     2401 ctttacgcgc catgcgtttg gtttattatc ttgcctggct aatgcggcgt tgggctgatc
     2461 ccgcgttccc gaaaaatttt ccgtggttaa ccggggaaga ttactggctg cgacagacgg
     2521 cgacttttat agaacaggca aaagttctac aagaaccccc tttgcaatta acacctatgt
     2581 attaatcgga gagagtagat catgaaaaag atttggctgg cgctggctgg tttagtttta
     2641 gcgtttagcg catcggcggc gcagtatgaa gatggtaaac agtacactac cctggaaaaa
     2701 ccggtagctg gcgcgccgca agtgctggag tttttctctt tcttctgccc gcactgctat
     2761 cagtttgaag aagttctgca tatttctgat aatgtgaaga aaaaactgcc ggaaggcgtg
     2821 aagatgacta aataccacgt caacttcatg ggtggtgacc tgggcaaaga tctgactcag
     2881 gcatgggctg tggcgatggc gctgggcgtg gaagacaaag tgactgttcc gctgtttgaa
     2941 ggcgtacaga aaacccagac cattcgttca gcatctgata tccgcgatgt atttatcaac
     3001 gcaggtatta aaggtgaaga gtacgacgcg gcgtggaaca gcttcgtggt gaaatctctg
     3061 gtcgctcagc aggaaaaagc tgcagctgac gtgcaattgc gtggtgttcc ggcgatgttt
     3121 gttaacggta aatatcagct gaatccgcag ggcatggata ccagcaatat ggatgttttt
     3181 gttcagcagt atgctgatac agtgaaatat ctgtccgaga aaaaataatt aaatgtaaat
//
LOCUS       DAZFXT010000246         3179 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.247, whole genome shotgun sequence.
ACCESSION   DAZFXT010000246 DAZFXT010000000
VERSION     DAZFXT010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.247"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(13..438)
                     /locus_tag="ACHKW0_004943"
     CDS             complement(13..438)
                     /locus_tag="ACHKW0_004943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7477598.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(422..664)
                     /locus_tag="ACHKW0_004944"
     CDS             complement(422..664)
                     /locus_tag="ACHKW0_004944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7477599.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            1056..1394
                     /locus_tag="ACHKW0_004945"
     CDS             1056..1394
                     /locus_tag="ACHKW0_004945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HFO7477600.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPNRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQDI"
     gene            1687..1839
                     /locus_tag="ACHKW0_004946"
     CDS             1687..1839
                     /locus_tag="ACHKW0_004946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HFO7477601.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTEAGAHRW
                     LARNSD"
     gene            1851..2489
                     /locus_tag="ACHKW0_004947"
     CDS             1851..2489
                     /locus_tag="ACHKW0_004947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477602.1"
                     /translation="MEFKDLPPSIQEIAAHTLRHRLNELELESVTKKDTDNMARNVRD
                     AFTGLYFCASINKHDSESVANKIAETTAQNINTKPTEEEIDQFAHDAGLKNKKEKSPY
                     AGNMFVYDNLIRIRGEIPAEYLARVHQALLKNLETELFDGNTNGFFMVSGLDKDWDAE
                     KRWNVATWLFSNRAAALEASACICGLFLTDHKYNLDVYSYIYAEHGPLWIDW"
     gene            complement(2490..2699)
                     /locus_tag="ACHKW0_004948"
     CDS             complement(2490..2699)
                     /locus_tag="ACHKW0_004948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477603.1"
                     /translation="MPNKKRNPLIEKQIECLVNQLRQSGLLKTHSELRLTESAFDDKL
                     NNVLYNGIIDFNRSVGRRGPAGVSL"
     gene            complement(2998..>3179)
                     /locus_tag="ACHKW0_004949"
     CDS             complement(2998..>3179)
                     /locus_tag="ACHKW0_004949"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477604.1"
                     /translation="SRYARKRPLVLTVPRALSRIHRITKFTLKRAAEQPRSKTDTAKR
                     HQKIDNRGRCSGVVT"
BASE COUNT          889 a          727 c          719 g          844 t
ORIGIN      
        1 ggctattgtc agtcaatgta ctgccacagc atttcctgcc gggccaccac ggttcatctg
       61 attgaaaccg gcgattgcca ctgcgacaaa atcatcagcg tctctcacca gtcgctcccg
      121 cgtctccacc agctcccgaa aataagctga actgtggctg cgcattctgg ccaccagcaa
      181 aggtggcatt gccttttcga tcgctggtaa caacgcctga attttttcaa ctgcatcagg
      241 ggtgtctttc tctacccagc ggaaaatttt ctgggtattg cgagccaggg cttccggatg
      301 gctgtcgtca tacagttcag gaaacgtcat acccaactca aaataagcct gggttattcc
      361 agctgccgga actttttcgc catcaggacg cgcccaggca ttcatcgcca tgcggatgtg
      421 ttcatgcttg attttcatga atcccccctt ggttagaagg cggattatga tcagaaccgg
      481 gaatgacaac cgtcggtatg tgtaactcat atttgagcgc cccggcagtg actgcctgaa
      541 ttagcaacgc ccatttccac ggaacctctt ccccccacat gctgactgtg gtttttgacg
      601 ttcctagagc tgcggctgtt ttaacaactc cgccaaaata gcctaatact tctgattttt
      661 tcatgagtcg ctccataaaa ctgaacgcca aaagtttaat aatcaaaacc aaagaaagtc
      721 aagaaacaaa accatctgtg ttttaaaatc aaaacatgag caagcaaaca atatctgaac
      781 gcataaccca acgtatgcat gcgctaaacc tgaaaggcaa agaccttgtc aatgccactg
      841 gcgcatcaaa aggctccgta agtcaatgga tgaacggtgg aggagcgccg tcctcgcgtt
      901 acataagttc actggcaaaa atattgaaag taaacgaaaa ttggcttctt aatggaggag
      961 agttaaatac aggtgattcg cttgatctat ctttaccgcc gataaaaacg gttccgctac
     1021 tatcacttca gcaggcagca agctggagtg attatatgaa aaattcctca ataacctctt
     1081 gtgtgcagct tgtcggagaa atcccggcca atacctttgc agttgttcta gagagtgaca
     1141 gtatgtcaac atctggtggg ggagtttcca tcccaaatgg ttcaacagtt tttgttgatc
     1201 ccaatcgaac cgtacaacca ggaaatattg tccttgcctt acccaaaggg accacaacac
     1261 ctgtcattcg taaactggag atagaagggc cggatattct tttagtcccc acgaatcctc
     1321 gctacccttc aattatgctg gatgatctat cttgcatatt gggcgtatgc tttaaaattc
     1381 aacaagatat ttaaccgacc tcatctattt gattaactgt atgccatcgt ggtgatggct
     1441 taacagctgc ctgcttaaaa tgttttgata aaaaaacatt gacctgaaaa gttcattttt
     1501 ctaaacttca ttcattccct ctccccaccc cacagaatgc agggcaatac ttcgagttac
     1561 caggcagtgg tcaggggtta agtagccagc ccgaggcgta agaacatgac ggcagggttc
     1621 aactttaata actatgcagc aggtttttgt tccgctaccc cggcgttaag gggaaatgag
     1681 gtcagcatgg atactatcga tcttggcaac agcgaatctc tggtatgtgg cgtgtttccc
     1741 aaccaggacg gtacgtttac cgcgatgacg tataccaaaa gtaaaacgtt taaaaccgaa
     1801 gctggcgcgc atcgctggtt agcaagaaac tccgactgat gaggttgacg atggaattta
     1861 aagatttacc tccttcaatc caggagattg cagcacacac acttcgtcat cgtctgaacg
     1921 aacttgaatt ggaatcggta acgaaaaaag acactgataa tatggctcgt aatgtgcgcg
     1981 atgcgtttac cggattgtat ttctgtgcgt ctataaataa acacgactca gagagtgtgg
     2041 caaataaaat tgcagaaacg acagcgcaaa acatcaatac gaaaccaacg gaagaagaaa
     2101 ttgatcagtt tgctcatgat gctggtttaa aaaacaagaa agaaaaatcg ccatatgcgg
     2161 ggaacatgtt tgtttatgac aatctcatca gaattcgtgg cgaaattccg gcggaatacc
     2221 tggcaagagt ccatcaggca ttgcttaaaa atttggaaac agaattattt gatggcaaca
     2281 ctaacggttt cttcatggta tcaggccttg ataaagactg ggatgcagaa aaacgctgga
     2341 atgttgctac atggttattc agtaacagag ccgctgcact ggaagcttcg gcatgtattt
     2401 gcggcctgtt cttaacagac cacaaatata atctggatgt gtacagttat atttacgctg
     2461 aacacggtcc actctggatt gactggtaat tataaggaaa caccagcagg gccgcggcga
     2521 ccaacagaac gattaaaatc aataatgcca ttataaagga cattatttaa tttatcgtcg
     2581 aatgctgatt ctgtgagcct caactctgaa tgagttttta ataaccctga ttgcctgagt
     2641 tgatttacca ggcattcaat ctgtttttca ataagcggat ttcttttttt gtttggcatt
     2701 ttatcctcca ttgaggttct gggttaaaaa tggagaccaa cacgctgtca cgtgtggtcg
     2761 tgcgccggac acggataaga atccggtact gacagtttac tgaaaggata tttccctgaa
     2821 aagtcagggc ataacgcgaa agcgtacggc gaagctcttt cccttagaag gcttgtcgtt
     2881 agatttcttc gaacgtgcgc ttccggttgt ggcactccgc gaaatggcgc ggcggtaagt
     2941 atggcggggt tattccttcc ccgttgagga caccgggttg tcaggttgac aatacgctta
     3001 agtgacaacc ccgctgcaac gccctctgtt atcaattttc tggtgacgtt tggcggtatc
     3061 agttttactc cgtggctgct ctgccgccct ttttaaagtg aattttgtga tgcggtgaat
     3121 gcggctaagc gcacgcggaa cagttaaaac aagcggtctt ttacgggcgt aacgggaat
//
LOCUS       DAZFXT010000247         3151 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.248, whole genome shotgun sequence.
ACCESSION   DAZFXT010000247 DAZFXT010000000
VERSION     DAZFXT010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.248"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..568
                     /locus_tag="ACHKW0_004950"
     CDS             <1..568
                     /locus_tag="ACHKW0_004950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HFO7477605.1"
                     /translation="EAVWVKQALEKNQPGKPVRVINPDDESIRIFSWLADNFPDLQYF
                     KLQLLDASNLRLTVSKQRNAITQQLIDNLIKGLLQTMPYASNISIAVLDDNVLESQAI
                     ETLSAIGLSYEKYKTANNVYFNIIGTLSDSELNKINNYVDEYYKQWGKQYVRFNVNLK
                     NQDTNNSSFSYGDNRFEKSQGSNWTFQE"
     gene            582..822
                     /locus_tag="ACHKW0_004951"
                     /pseudo
     CDS             582..822
                     /locus_tag="ACHKW0_004951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            842..1173
                     /gene="sctI"
                     /locus_tag="ACHKW0_004952"
                     /pseudo
     CDS             842..1173
                     /gene="sctI"
                     /locus_tag="ACHKW0_004952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311744.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
     gene            1170..1904
                     /locus_tag="ACHKW0_004953"
                     /pseudo
     CDS             1170..1904
                     /locus_tag="ACHKW0_004953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
     gene            1920..2501
                     /locus_tag="ACHKW0_004954"
     CDS             1920..2501
                     /locus_tag="ACHKW0_004954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030257 - type III protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0030254 - protein secretion by the type III
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HFO7477606.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSLNNTPINNPVLRSITNEMIVLQ
                     YNLSVEHFNLNSSLIYYINNWNLFPLFCLFSGYHFYRERFAERGFFYKVPAVLRDYLS
                     AIPVKINEKARYKPGIASYHNIITCGFSTLSPYIRQQPLAMQQRFNLLFPDFVDHIQL
                     PLPLASTLLERITFYAKKNRDELDKISCKWCCD"
     gene            2530..2715
                     /locus_tag="ACHKW0_004955"
     CDS             2530..2715
                     /locus_tag="ACHKW0_004955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477607.1"
                     /translation="MVSLKESILLETSAKIQSIEVKREEKFIQGYYDGYTKGIIDVMD
                     NFIPLISLLSSELEKKE"
     gene            2718..3149
                     /locus_tag="ACHKW0_004956"
     CDS             2718..3149
                     /locus_tag="ACHKW0_004956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001701978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477608.1"
                     /translation="MINDLKSILLKSSEEVDVFIKIFEGWRKKLPAISGPVNLFIPTR
                     FKDKYLEVESYFVDKSIWNVHITFHDDKRFVFFTDQFIAEFSPQEFVDNCEQYLINNH
                     CFSPDKVNEICEQARHYLVEKMFETHSLDMNNSVLASPEDL"
BASE COUNT         1079 a          499 c          595 g          978 t
ORIGIN      
        1 tgaagcagtc tgggtcaaac aagcactgga aaaaaatcaa ccaggaaagc cagtacgggt
       61 gataaatcct gatgatgaat cgatacgaat tttttcatgg cttgctgata atttccctga
      121 tttacaatat tttaaacttc aactattaga tgccagtaat ctaagattga cagtgagtaa
      181 gcaacggaac gctatcacac agcaactaat cgataatctt attaaaggat tactgcaaac
      241 tatgccatat gccagcaata taagtattgc ggtattagat gataatgtat tggaaagtca
      301 ggctattgaa acgttgtcag cgataggtct ttcttatgaa aaatataaaa cagctaacaa
      361 tgtgtatttc aatatcattg gtacgttaag tgacagtgaa ttaaataaaa ttaataacta
      421 tgttgacgaa tattataaac aatggggtaa acaatatgta agatttaatg tgaatttgaa
      481 aaatcaggac acaaataata gttcatttag ctacggagat aaccgattcg agaagtctca
      541 aggtagcaac tggacatttc aggaataatt gaggaggact tatggcagat tggaatggtt
      601 atattatgga tatcagcaat caatttgatc agggcgttga tgatctgaac cagcaagttg
      661 aaaaagcgtt ggaggattta gccaaccaat ccctccgaca cgaaatttct tgccgaatat
      721 caaagtgcat tagctgagta tacattatat cgaaatgcgc aatctaacgt tgtaaaagcg
      781 tataaagatc ttgattcagc aatcatacaa aacttccgct aaactggata aggattttat
      841 tatgtcagta agcaatatgc cacctattga tagagctgag caatcaactg ctcatgaaat
      901 acagcaagcg aaagtgattg atttaaatga ccgggttctc aatcttgaca acccggatga
      961 taaaatgatc tccgcttttg ccaactatgc tgtacaaact gaaaattggc agcaaaatgc
     1021 gttacaagca ttaaggtctg ataaagaggg gttaactcct gaaaaattat tagtcttgca
     1081 ggatcatgtg cttaattata atgtcgaggt ttcattggtg gaacattagc aagaaagatt
     1141 gtcgctgcag ttgagacgtt gacccgctca tgaaatatat atcattatta ttgtttatcc
     1201 tgctgttgtg cggctgcaag cagcaggaat tattgaacca tcttgatcaa cagcaagcta
     1261 atgatgtcct tgctgtttta caaagacata atattaatgc tgaaaagaag gattaaggca
     1321 aaacaggatt ttcaatattt gtcgaaccaa cggattttgc ttctgcggtg gattggctaa
     1381 aaatctacaa tctaccgggt aagcctgata ttcaaatatc gcagatgttc cctgcagatg
     1441 cactcgtttc atctccacga gcggaaaaag caaggctata ttctgctatt gagcagcggt
     1501 tagagcaatc tctcaaaatt atggatggca ttatttcttc gagagtacat gttagttacg
     1561 atgttgataa tggcgatagt ggtaaaactg ctttaccaat tcatatttct gtacttgcag
     1621 tatatgaaaa agatatcaac ccagaaataa aaataaatga tatcaagaga tttattgtta
     1681 atagctctgc aagcgtacaa tatgaaaata tttctgttgt attgtcaaaa cgaagagaca
     1741 ttattgagca agcaccaaca tatgaaatta gcgaacctgt ttttgcttat gataaggcta
     1801 tgccggtttc tatcttactt gcattaatat ccgtagcgac ttgctggctg ttatggaagt
     1861 atagggccat tctaactaat ttagtcaggt tgaaaattaa gtagacatat gagagcgtta
     1921 tgaatcttgc cctaagaaag attatatatg atccaattag ttatattcat cctcaacgag
     1981 taagcttgaa taatacacct attaacaatc cggtgcttag atctattacc aatgaaatga
     2041 ttgttctgca gtacaatctt tcagttgaac attttaatct gaatagttca cttatttact
     2101 acataaacaa ttggaattta tttccattgt tctgtttatt cagtggttat cacttttatc
     2161 gagaaaggtt tgcagaacgc ggattttttt ataaggttcc tgctgtgcta agggattatt
     2221 tatcagctat accggtgaaa ataaatgaaa aggcacgata caaacccggc atcgctagct
     2281 atcacaatat catcacatgt ggtttttcaa cgttgtcacc ctatataagg caacaaccat
     2341 tggcaatgca gcaacgattt aatttattat ttcctgattt tgttgatcac atacaattgc
     2401 cgttaccact tgcatcaact cttttagaga gaattacatt ttatgcgaaa aaaaatagag
     2461 atgagcttga taaaatctcc tgcaaatggt gttgtgatta aaagaaaaat ttctgatgga
     2521 ttgaaataaa tagtttctct taaagaaagt atccttcttg aaaccagcgc aaaaattcag
     2581 agcattgagg taaagagaga agaaaaattt attcaaggtt attatgatgg ctatacaaaa
     2641 ggcattattg atgtgatgga taattttata cccttgataa gcttgttgag ctcagaacta
     2701 gaaaaaaaag aataaacatg ataaacgatc tgaagagtat attgttaaaa tcctcagagg
     2761 aagttgatgt ttttatcaaa atttttgaag gttggagaaa aaaattaccg gcaatatcag
     2821 gtcctgtaaa tctctttata cctaccagat tcaaagataa gtatcttgaa gtagagtcat
     2881 attttgtcga caaatcaata tggaatgtcc atattacatt tcatgatgat aagcgttttg
     2941 tgttttttac cgatcaattt attgctgaat ttagtccgca ggaatttgtg gataattgtg
     3001 agcaatattt aattaataat cattgtttct cccctgacaa ggttaacgaa atctgcgaac
     3061 aagccagaca ttatttagtt gaaaaaatgt ttgaaacaca ttctttggat atgaataatt
     3121 ctgttttggc aagccctgag gacttatagc t
//
LOCUS       DAZFXT010000248         3117 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.249, whole genome shotgun sequence.
ACCESSION   DAZFXT010000248 DAZFXT010000000
VERSION     DAZFXT010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3117
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.249"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(97..1956)
                     /gene="gss"
                     /locus_tag="ACHKW0_004957"
     CDS             complement(97..1956)
                     /gene="gss"
                     /locus_tag="ACHKW0_004957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HFO7477609.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2161..3027
                     /gene="yghU"
                     /locus_tag="ACHKW0_004958"
     CDS             2161..3027
                     /gene="yghU"
                     /locus_tag="ACHKW0_004958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HFO7477610.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
BASE COUNT          740 a          829 c          786 g          762 t
ORIGIN      
        1 aatgaataaa tattggaata tataaatatt gaatattttg attaatcccc gtactgatta
       61 ttcttcataa tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg
      121 ttcaatatca ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc
      181 gtagttgccg ccaacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa
      241 acaccacagt tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag
      301 cacctcttca tgatggctga cgaggtcgat attgctgcca cagcgaccgg cgatcggttt
      361 cactgcgtaa cctgttttca ccagttcatc attaacagtg aaatcggtat ccagcaggta
      421 acgatggtgc gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac
      481 cgtccacagc ggctcaaaga ccagcacttc cgggcgcagc aatacgtcga taagacgcac
      541 ttcgttttgc ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc
      601 acgaatctga tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagtcg
      661 cccttcccca tcaatcagtt gcccggcagc atcccagccc agttcatcca gcccgcgcaa
      721 gatacgcgtt tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt
      781 ttcctcgata tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca
      841 ggcaccagcc agttcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc
      901 ctgctccgcc caacgttcga ggatcaagcc cgcttcggta tgacatgagg cagagtcggc
      961 gttgtactcg taaaccttca gaccacgctc atccatgcag aaatccatac gaccggtgat
     1021 catatggtga cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat
     1081 gtcgaacagc gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat
     1141 caggtgcagc tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta
     1201 gtaatgataa ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag
     1261 cggatctttt tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc
     1321 gctgattttc agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc
     1381 cgtctggatc atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca
     1441 gccgttttcg accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga
     1501 atgaatcacg ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc
     1561 gacatggcca gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac
     1621 cggcgcacgc ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac
     1681 ttctcgcagg aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac
     1741 accgtaattc agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat
     1801 atattcgtcg tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag
     1861 agaactgtaa tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc
     1921 gaacggggca tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc
     1981 taagcagttg gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga
     2041 ggtcagacta atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat
     2101 tctgtacagc aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact
     2161 atgacagaca atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc
     2221 gcgttcgcca atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt
     2281 ggcaaacacc cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt
     2341 atgcttgagg agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt
     2401 cgtattggcg atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag
     2461 atcccggcgc tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg
     2521 atcctgcttt atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt
     2581 actgaaacga tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt
     2641 tttggtcact tttaccatta cgcgccgatg aaaattgagt acgccatcaa ccgctttacc
     2701 atggaagcca aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttatt
     2761 gcgggcgatg agtacaccat tgcggatatg gcgatttggc cgtggtttgg caatgtggtg
     2821 ttaggcggcg tgtatgatgc cgcagagttt cttgatgcgg gcagttataa gcatgtacaa
     2881 cgctgggcga aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc
     2941 accaacggac cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg
     3001 aatacggaag ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc
     3061 cggataaggc gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctg
//
LOCUS       DAZFXT010000249         3083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.250, whole genome shotgun sequence.
ACCESSION   DAZFXT010000249 DAZFXT010000000
VERSION     DAZFXT010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.250"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1469)
                     /locus_tag="ACHKW0_004959"
                     /pseudo
     CDS             complement(1..1469)
                     /locus_tag="ACHKW0_004959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1415..1636)
                     /locus_tag="ACHKW0_004960"
     CDS             complement(1415..1636)
                     /locus_tag="ACHKW0_004960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477611.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVT"
     gene            complement(1681..>3083)
                     /locus_tag="ACHKW0_004961"
     CDS             complement(1681..>3083)
                     /locus_tag="ACHKW0_004961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7477612.1"
                     /translation="AQSITSFHKEKSAMNTGEQIKSFENKRAALAASLEEIMNKAAEE
                     GRTLDVEEEEHYDNTAAEIRQVDAHLKRLRELETSKAATAQPVKQAGNGNVATVASAP
                     VIRVEQKLEKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGAGTT
                     TDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSGGAA
                     GWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEAVVA
                     RLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVTANLQPTGAVW
                     LMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAPDIYLA
                     DDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWRRRRTA
                     AVAVITGVNYGTASGG"
BASE COUNT          610 a          992 c          781 g          700 t
ORIGIN      
        1 ttgtgcctgc gcggccatct tcccctcaaa tgtgcgctgt aaatccgcca gcaccgtgga
       61 gaattcttcg cccagtgcac gaataatgga cagttcccgt tccgtcattt tctcagtatc
      121 cccctgaaca tcgctttcac tgcatcatgc tctgtttcac tgattgcctt attaccgtca
      181 gatgcgccgt caggcagttg tgctgaaact gttttcccgg ccgacgcgaa cgggtcctca
      241 cgggcatcac gacgggacag cgcctccaga ctgtagttct gctgctgaag atacagtgca
      301 tcaccgccgg caaggggcgg caggttctca cgtttacggg cctcattggg cgtgagaagc
      361 gtatttttca ccgattcacc cagtgttttc atgcgccgtt cgctgtccat tctcagcagc
      421 gtggtgacgt caaactccgt gctctcgttt tcccccgttt ccagcgcctc atccagtaac
      481 agctcaatgg actcaatcag cgtctgcaga cactgggaat aatactgctg ctccagcgcc
      541 tccacgttgt cactggaagg cgggtggcca acgccaatct tgtaggccgg gacacggaac
      601 accgaacaga caatttcagc ggtcatcttc agttgttcca ccgtctgcgc atccacaggt
      661 gaaaacgtcg tggggttgta ttttgccccg ttgctcagaa tggccgtttt ccccgcattt
      721 tcgcctgtat acccgctgtc ccagttgctc ttcagttttt tcgcattttc ttccgtaata
      781 ctgccgggga tctcaatcac cccggacggc ctgccgccat ttctgaaaaa agacgtcgaa
      841 ttttcctgaa tatgatgccc ctgcgtggcc gccagcccgg cggcatacac cggcggcagc
      901 cccacaagcg gatgaaaaaa acagttaaaa cggtcgtgga tcacctcccg ggcaggcacc
      961 gtcaccgact ctgtgatccc acagttccgg tccggcgtga tgcggtagaa cacgtcgccg
     1021 tcatccgcca ccagaggttc aacccggttc cagtccagaa tacgcagttc tttgatctgc
     1081 ccccgggggt tgcggatttt cagcaccacc gtattgccgt gacgcagttt ggcgttcagc
     1141 cacagttcaa aaaactggat gcgattctgc tgcgcattag gacgacgaca gagacgggca
     1201 gtatctccct gccgcttttc ccggcgtatt ccctgtacat cagtctgcat caggcgaagc
     1261 cgcattttgg cgatatcctg ggatatcagc gaaatgcatg aaaacaccgc gtgaaaggac
     1321 aaaacggttt ccggatcggc tttcacaccc tgctgccagg caccggcaaa aggctcagcc
     1381 accgcctgaa acagggaccg ccagcccact tctcttacgt cacgtcctga tttctggttt
     1441 tttcgggttc gccgcaaaag gttccacatt cgccatgctc cgcatcacgt ttctttttct
     1501 gacctgccgg acgtcgcgct gtgatgtact ccgccttccc caggcgaacc agcacctccg
     1561 cacacggctg tgccacatca cggatatccc cggcccgggc atcatgcgtg ccctgcagat
     1621 actggatttt tgccatctgt tactgcggga agctcgcgcc tcccgccctc ctcatcagac
     1681 tcagccaccg gacgcagttc cgtagttcac accggtgatc accgccactg ccgcggtacg
     1741 gcgacgacgc cagttgatcc agcgctccgc acggatggcc acgctgccgg tctgaaacat
     1801 ggagaccagt tccaccggtg acggtgtgct gctgtcgctg gtcggttcag actgcatctc
     1861 cagtgacgct tcacgggaca tatccaccgc cacaccgccg tcatcagcca gataaatatc
     1921 cggtgcattc accagtacca gctggtcacc cacatactgg gagacaatca ccggcagccc
     1981 ctggaaggtc ccgcccagca aggtcatgtc cggatattcc ttctgcccca gcgcattttt
     2041 acgcatggac agcgccaggg cattggtgct ggacatcagc cagaccgcac cggtgggctg
     2101 cagatttgcc gtcacaaact ggccaaacgc ggcctcggca tccgcatccg ggttaccggt
     2161 tgatgccgtg cccttcacat catgggtgat ggacgccggg gagacatccg ccaccgccgc
     2221 ttttttcggg tccacaaagt ctgtgtccag acgcgccacc accgcttccg ccagcgcatt
     2281 acggaccagt gcatcagcag ccggactgga aaaacggatc aattcttccg tcagtaccgc
     2341 aatggccgac actttcgcat gactgaaggt gatggattca aaatcaaact tcgtcagggg
     2401 tctggcctta ccctcaccca cccagccggc agcaccaccg gacacctggg catgcacgcg
     2461 gatattgaac ggcacctgac gaagtgcagg gatcccgccc tgaccaaatc gcccgataat
     2521 ggtctgcgga cgcaggtaat caataaagtc ctgcgcatat tcctggtatt cagacaggct
     2581 gcctgcccac tgcgggtccg tggtggtccc tgcccccacc gccgatttca ggacatgatg
     2641 cagacggctg tcatccggat actgacgacg ggccacttcc agggcttcag agcggacacc
     2701 tttagccgcg gccagtgatt tggcaaagcg ggcgaaacca atccccttct ccagtttctg
     2761 ctcaacacgg atcaccggcg ctgaagccac cgtggccaca ttcccgttac cggcctgttt
     2821 caccggctgt gccgtggcgg ccttactggt ttccagttca cgcagacgct tcaggtgcgc
     2881 atccacctga cggatttccg ctgcggtgtt gtcgtagtgc tcttcctctt ccacatccag
     2941 tgtgcggcct tcctctgcgg ctttgttcat gatctcctca agggaggctg ccagcgccgc
     3001 acgcttgttt tcaaaacttt taatctgttc accagtattc attgctgact tttccttatg
     3061 aaaagaggtt attgactgtg ccg
//
LOCUS       DAZFXT010000250         3039 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.251, whole genome shotgun sequence.
ACCESSION   DAZFXT010000250 DAZFXT010000000
VERSION     DAZFXT010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.251"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..226
                     /locus_tag="ACHKW0_004962"
                     /pseudo
     CDS             1..226
                     /locus_tag="ACHKW0_004962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            451..942
                     /locus_tag="ACHKW0_004963"
     CDS             451..942
                     /locus_tag="ACHKW0_004963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HFO7477613.1"
                     /translation="MSTETIEIFNNSDEWANQLKHLLSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNVTDAEIYYKFLCIYAFENHEYLEGFASVCQSKKKYQQAYDLYKLSYN
                     YFPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            1276..2652
                     /gene="ygeH"
                     /locus_tag="ACHKW0_004964"
     CDS             1276..2652
                     /gene="ygeH"
                     /locus_tag="ACHKW0_004964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HilA family transcriptional regulator YgeH"
                     /protein_id="HFO7477614.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPMAA
                     TNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPVDE
                     LHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKELYDFTLESIYR
                     AMTIFDRLQNKSDIQTLKTECYCLLAECHMSLALHGKSELELAAQKALELLDYVSDIT
                     TVDGKILAIMGLITGLSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEAR
                     ICIDKSLQLEPRRRKAVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLK
                     QLTRICMR"
     gene            2820..3038
                     /gene="ygeI"
                     /locus_tag="ACHKW0_004965"
     CDS             2820..3038
                     /gene="ygeI"
                     /locus_tag="ACHKW0_004965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgeI"
                     /protein_id="HFO7477615.1"
                     /translation="MTNPIGINNLSQSSNIANATGDEVVSLDKHINTSATDTDQIQAF
                     IVSTWMAPFQNDMYSEDNPISPYYKIEW"
BASE COUNT         1142 a          497 c          490 g          910 t
ORIGIN      
        1 atttaatgag tatggctgta aatgaactca taaaactgac caaaatagag aataatgtag
       61 acctgaataa attcactact atatgcacag acgttctatc ccccctcgtc accagacata
      121 ataaagaaaa aaaacaaacg acattctacc cttcgcaaaa atcccctttt taattttcat
      181 tgaaaaaatg gcaattgata ctatgaatcc aggtcataca cattaaggca tttatgtaat
      241 gtcgtaatta agacaactaa taaggtgaat attagtaaaa caactaatga tgttattatt
      301 ttgtagtgaa cagtactttt accaataatg aaaaatatac cataagcaat gcaagcttaa
      361 aaccaataat ttaaaaaaga taaatgtaaa aatattaatg agtcaatcaa taataattta
      421 gcatcacgag acacatcaca gaggaatatt atgagcacag aaacaattga aatattcaat
      481 aatagtgatg aatgggcaaa tcaactaaaa cacctattat cgaaaggaga aaatctggca
      541 ttactacatg gtttaactcc tgatatcctt gatagaatat atgcatatgc attcgactac
      601 catgaaaagg gtaatgtaac ggacgcagaa atttattata aatttttgtg catttatgcg
      661 ttcgaaaatc atgagtatct agaaggtttt gcatcagtat gtcagtcaaa aaagaaatat
      721 caacaagcat atgaccttta caaactaagt tacaattact tcccgtatga tgactattca
      781 gttatttatc gtatgggtca atgtcagatt ggggctaaaa atatcgataa cgcaatgcaa
      841 tgtttctatc acattattaa caattgtgag gatgatagtg ttaagagtaa agcgcaggca
      901 tatattgaac tcttaaacga taattcagaa gataatggct aagcattaca atcatttggt
      961 taaatttgct ggttatagtg cagttaacca ctataaccag cacctcttat gttttcttaa
     1021 atcagcatct taaaacagtt aagagcatac tataaatcat tatttttcaa tgcgttagag
     1081 aacaatctac tttatcaaaa ttgaataagg ctatttcaga ttacatcact gaattcctaa
     1141 gataaattag tgaacacaac catagaatgt cgaatgtaat ttcctgtagc gttaatgaca
     1201 cataaatatg aatagccata atttctattg cttaagcagg atgcaagaaa ccaatttttt
     1261 catagaggtt aactaatgga cttagaaaat aaattctcat atcattttct tgagggatta
     1321 acgctcacgg aagatggaat tcttactcaa ggaaatgagc aagtttatat tccacagaaa
     1381 gagttaggtg tattaatagt attacttgaa tccgctggtc atgttgtact gaaagatatg
     1441 atcatcgaat cagtatggaa aaatattatt gttagtgacg agtccctgac aagatgtatc
     1501 tattctttgc gctgcatttt tgaaaaaatt ggctatgatc gttgcataga aacaatctac
     1561 cggaaaggtt atcgtttcag cgggcaggtt ttcaaaacta aaattaatga agataatact
     1621 tcagactatt ccatagctat attccctttc actacttcgt tgaatacact ggatccatta
     1681 atacttaatc aggaattagt gcaaatcatt tcaaataaaa aaatcgatgg tctctatacc
     1741 tatccgatgg ctgcgacaaa tttttgtaat gatcacatat ctcaaaattc attcttgagc
     1801 agattcaagc cagattattt cgttacagga agaataaacc agaataatgc agtgaacact
     1861 ttatacattg agttgatcga cgctaaaaac cttttcctca tcgccagtaa tcatctccct
     1921 gttgatgaac tacataatac atcacaattt attatagata atatccttca aacggtacat
     1981 aaaccagaac gatctgtaag attagctaag caggaccaag gatataagaa tcattattta
     2041 tcagatgaaa tgttagccgg aaagaaagaa ctttacgact tcacccttga aagcatttac
     2101 agggccatga ctatatttga tagattacaa aataaaagtg atatacagac gctaaaaaca
     2161 gaatgttatt gccttctagc ggaatgccat atgtctttgg cacttcatgg aaaaagtgaa
     2221 cttgaacttg ctgctcaaaa agcattagag cttttagatt atgtatcaga cataaccact
     2281 gtcgatggaa aaattttagc tattatggga ctgataactg gtctgtctgg acaagcaaaa
     2341 gtatctcata tcttatttga acaggctaag atacactcaa ctgatatagc ctctctctac
     2401 tactataggg cactagtcca ctttcataat gaaaaaattg aagaggcaag gatttgtata
     2461 gacaaatcac tacaactcga acccagaaga cgaaaagcag ttgtgataaa agaatgtgta
     2521 gatatgtatg tgcctaaccc gctcaaaaac aacattaaac tctactataa agaaactgag
     2581 agtgaaagcc atcgagttat aattgacaac attttgaaat taaagcagct gacgagaatt
     2641 tgtatgcgat aaatttttat tgacgcaatt ttattattta gatgtatgca acttgttatt
     2701 taaacaacag agataaaaat atctaagata ttcaccttat tgcaagatat ttaaaatgct
     2761 ctagagttaa aagtatgatg taaagaccat tggcctggac aattaaaaaa gggagcttta
     2821 tgactaatcc aatcggtatc aataatttat ctcaaagttc aaatatagcg aatgcaacgg
     2881 gtgatgaagt cgtatcatta gataaacaca tcaacaccag tgctactgat acagatcaaa
     2941 tacaagcatt tatagtttca acatggatgg cgccttttca aaatgatatg tattcagaag
     3001 ataatcctat ctcaccttac tataaaattg aatggtaga
//
LOCUS       DAZFXT010000251         3028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.252, whole genome shotgun sequence.
ACCESSION   DAZFXT010000251 DAZFXT010000000
VERSION     DAZFXT010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.252"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(151..227)
                     /locus_tag="ACHKW0_004966"
     tRNA            complement(151..227)
                     /locus_tag="ACHKW0_004966"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(191..193),aa:Asp,seq:gtc)
     gene            complement(360..1091)
                     /gene="dnaQ"
                     /locus_tag="ACHKW0_004967"
     CDS             complement(360..1091)
                     /gene="dnaQ"
                     /locus_tag="ACHKW0_004967"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HFO7477616.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1156..1623
                     /gene="rnhA"
                     /locus_tag="ACHKW0_004968"
     CDS             1156..1623
                     /gene="rnhA"
                     /locus_tag="ACHKW0_004968"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HFO7477617.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHSWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1620..2342)
                     /locus_tag="ACHKW0_004969"
     CDS             complement(1620..2342)
                     /locus_tag="ACHKW0_004969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7477618.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQYRKPQA"
     gene            2376..>3028
                     /gene="gloB"
                     /locus_tag="ACHKW0_004970"
     CDS             2376..>3028
                     /gene="gloB"
                     /locus_tag="ACHKW0_004970"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HFO7477619.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFL"
BASE COUNT          830 a          725 c          705 g          768 t
ORIGIN      
        1 aaatattaat gatatggtct accatatgtg attaacacat ttacagcata acaaaaccat
       61 agaccgaaga aatagcggga tttaaatgca aaaaaaaacc gcaaaattaa aaatcttgcg
      121 gttctctgaa ctcattttca tgagtgaatt tggcggaacg gacgggactc gaacccgcga
      181 ccccctgcgt gacaggcagg tattctaacc gactgaacta ccgctccgcg ttgtgttccg
      241 ttgggaacga ggcgaatatt acggattgcc tcacccttcg tcaacggttt ttctcatctt
      301 ttgaatcgtt tgctgcaaaa atcgcccaag ccgctatttt cagcgccttt tacaggtatt
      361 tatgcccgcc agaggcagct tccgcccttc ttctccacca gatcaagacg ggcttcatga
      421 gctgcaagct cttcatctgt cgcaaaaaca acgcgtaact tacttgcctg acgtacaatg
      481 cgctgaattg ttgcttcacc ttgttgctgc tgtgtctctc cttccatcgc aaaagccatc
      541 gacgtttgac caccggtcat cgccagataa acttccgcaa ggatctgtgc atcgagtaat
      601 gccccgtgta gcgttcgttt actgttatct atttcgtagc gggcacataa cgcatcgagg
      661 ctgttgcgct taccaggaaa cattttcctc gccaccgcaa ggctatcggt gaccttacag
      721 aaggtattgg tcttcggaat atcgcgctta agcaacgaaa actcgtagtc cataaagccg
      781 atatcgaacg ctgcgttatg gatcaccaac tccgcgccgc ggatatagtc catgaactca
      841 tcggctactt cggcaaacgt gggcttatcg agcaaaaatt catcggcaat accatgtacg
      901 ccaaaggctt ccggatccac cagccgatcg ggtttgagat aaacatggaa gttattgccc
      961 gtcaggcgac ggttcaccac ttcaacggca ccaatctcaa tgatcttgtg gccttcatag
     1021 tgcgcaccaa tctggttcat accggtggtt tcggtatcga gaacgatctg gcgtgtaatt
     1081 gcagtgctca tagcggtcat ttatgtcaga cttatcgttt tacagttcga ttcaattaca
     1141 ggaagtctac cagagatgct taaacaggta gaaattttca ccgatggttc gtgtctgggc
     1201 aatccaggac ctgggggtta cggcgctatt ttacgctatc gcggacgcga gaaaaccttt
     1261 agcgcaggct acacccgcac caccaacaac cgtatggagt tgatggccgc tattgtcgcg
     1321 ctggaggcgt taaaagaaca ttgcgaagtc attttgagta ccgacagcca gtatgtccgc
     1381 caggggatca ctcagtggat ccatagctgg aaaaaacgtg gctggaaaac cgcagacaaa
     1441 aaaccagtaa aaaatgtcga tctctggcaa cgtcttgatg ctgcattggg gcagcatcaa
     1501 atcaaatggg aatgggttaa aggccatgcc ggacacccgg aaaacgaacg ctgtgatgaa
     1561 ctggcccgtg ctgcggcaat gaatcccaca ctggaagata caggctatca agtcgaagtt
     1621 taagcctgtg gtttacgata ttgccgggtg gctccaaccg cctggcgaat tcgtggcttg
     1681 tttttactct gtttcatcgg atttagcgtt aaaggaatag tccgtttccg ggcaacaata
     1741 agttgtaagc aaccaagcgc aggaatatgt gcattcaata gttttccacc gtgtttgttc
     1801 cacgggagaa cgtggaaacg gctggcgtgc agcacttcaa aattcaacaa agagagccag
     1861 tccagttgcc gcatcagagt aaacatccgg ctgttatagg gcgaggtttt gcgcaatacc
     1921 ggcacaagtt tgcgtaatcc cattaaactg atgggattga agccactaat caccagccag
     1981 ccatcatcaa tcaatacccg atcggcttca cgcaataaac gatgcggatc ggtgcaccac
     2041 ggcaatgtat gtgccagtag acaaacatca acggatttat cggcaaaagg aagatgaagt
     2101 gggtccgcct gtacctggac gggcattcct tgcgcagaaa cattcacttg atgagaaacc
     2161 gcgcacgctt cgcaattgat ttctgcgctt aaattgccaa tcttaagcag atgaaaacca
     2221 tacattttag tgaaccacgg gttgagctgg cgctccagcg ccttgcgata aagctctccc
     2281 cagggcaaat cgccccagca atcaggagcc acgacagttt gagggactct tgccggtttc
     2341 atcacaacct tccgtttcac actaagaggt aatctatgaa tcttaacagt attcccgcct
     2401 ttgatgacaa ttacatctgg gttttgaatg atgaagcagg tcgctgcctg attgtcgatc
     2461 ccggagacgc agagccagta ttaaacgcca ttaccgccaa taactggcaa ccggaggcca
     2521 tatttctcac ccaccatcat cacgatcacg ttggcggcgt aaaagaactg gtggaaaagt
     2581 ttccgcaaat tgtggtgtat ggtccacaag agacacaaga taagggaaca acacaggtag
     2641 tcaaagatgg cgaaactgcc ttcgttttgg ggcatgaatt tagtgtaatt gccacaccgg
     2701 gtcacacttt aggacatatc tgttacttca gtaaacctta tctattttgc ggcgacacgc
     2761 tgttttctgg tgggtgtggt aggttgtttg aagggacagc atcacaaatg tatcaatcac
     2821 ttaataagtt aagtgcgtta cctgacgata cattggtatg ttgtgctcat gaatatacgt
     2881 tatcaaatat gaagtttgct ttgagtattc ttccgcacga tttgtccata aatgattatt
     2941 atcgtaaagt taaggagtta cgggcaaaaa atcaaataac actacccgta attctgaaaa
     3001 atgagcggca aattaatgtt tttttaag
//
LOCUS       DAZFXT010000252         3018 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.253, whole genome shotgun sequence.
ACCESSION   DAZFXT010000252 DAZFXT010000000
VERSION     DAZFXT010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3018)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3018)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3018
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.253"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(61..696)
                     /locus_tag="ACHKW0_004971"
     CDS             complement(61..696)
                     /locus_tag="ACHKW0_004971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012816780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO7477620.1"
                     /translation="MPVTTLSIPSISQLSPAGVQSLQDAARLESGIRISIGSGQYSVH
                     YVQLLDGFSVEPVRGGLLDRLLGREHRMERRAVALERQLNGGVDFLSSVNNYFQSVMA
                     EHRENKTSNKILMEKINSCVFRPDSNHFSCPESFLTCPITLDTPENGVFMRNSRGAEI
                     CSLYDKDALVQLVETGGAHPLSREPITESMIMRKDECHFDSKKEAFVASDA"
     gene            complement(764..1345)
                     /locus_tag="ACHKW0_004972"
     CDS             complement(764..1345)
                     /locus_tag="ACHKW0_004972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0044403 - biological process involved in
                     symbiotic interaction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector NleG family protein"
                     /protein_id="HFO7477621.1"
                     /translation="MPGLVSYISSTSFANEMAEMRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGLGLVYAELLNIGFNDGVDALRNRVLSVLPGMVAQRQENSLQAKISE
                     CTFTVDIEKLHCPGEVLQCPITLEQPEKGIFVKNSDGSDVCTLFDAAAFSRLTGEGLP
                     HPLTREPITASIIVKHEECIYDDTRGNFVIKGN"
     gene            complement(1636..2211)
                     /locus_tag="ACHKW0_004973"
     CDS             complement(1636..2211)
                     /locus_tag="ACHKW0_004973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO7477622.1"
                     /translation="MPLTSAIASNSFSTGMQVLRAQMAASGGGEITVGGQTVRITYSE
                     TDGRFLASGGNNSLLSGLLLTGLNGGPEALRDIMLRMVSGSGNTQSHGDIEGKISQCK
                     FSVNTESLQCPSEAVRCPIILDKPEEGVFVKNSEGSLVCTLFDSVSFSHLVRDGGKHP
                     LTREPITSSMIVSQEQCIYDQTKGNFVIKDK"
     gene            complement(2325..2594)
                     /locus_tag="ACHKW0_004974"
     CDS             complement(2325..2594)
                     /locus_tag="ACHKW0_004974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7477623.1"
                     /translation="MNILRKLMERLCGCGKHDDCEHGQSLTVQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
     gene            complement(2596..>3018)
                     /locus_tag="ACHKW0_004975"
     CDS             complement(2596..>3018)
                     /locus_tag="ACHKW0_004975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7477624.1"
                     /translation="QGPKGDRGERGETGLTGNAGPQGPKGDTGAAGPAGPQGPKGETG
                     AAGPVGATGPQGPKGDPGETQIRFRLGPASIIETNSHGWFPDTDGALITGLTFLDPKD
                     ATRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          808 a          822 c          531 g          857 t
ORIGIN      
        1 tatccttaca tggcactatt ataataaact attaatataa aaacacacca acagaaaaaa
       61 ttaagcatca cttgcaacaa aggcttcttt ttttgaatca aagtgacatt cgtcttttct
      121 catgatcatt gattctgtta taggttctcg actcagagga tgagctccac cagtttcaac
      181 aagttgcact aacgcgtcct tatcatatag agagcatatc tcagcacctc gtgagtttct
      241 catgaacact ccattctcag gtgtgtccag cgttatcggg catgtcaaaa atgactccgg
      301 gcaagaaaag tgattagaat ccggtctaaa tacacaagaa tttatttttt ccattaatat
      361 tttattactt gttttatttt ctctgtgttc tgccatgaca ctctgaaaat agttattaac
      421 actacttaaa aaatcgacac ctccatttaa ttgcctttcc agagccacag cccttctctc
      481 cattcgatgc tcacgcccca atagcctatc cagtaagcct cctctcaccg gttcaactga
      541 aaatccatcc agtagttgga catagtgaac agaatattgg ccactaccaa tggatattct
      601 tattccactt tcaagtctgg ctgcatcctg caaagactgc actcctgcag gagataattg
      661 agatatactt gggatactta aggtggtaac aggcataaaa acctcattca gtaattcata
      721 tcagtttgca ggatgtttca ttaaataaag ggtaatgttc atttcaatta ccctttataa
      781 cgaagtttcc tctggtatcg tcataaatgc attcttcatg ttttacaatt attgatgccg
      841 ttattggttc ccgggtcagt gggtggggta agccttcacc agtcaaacga gaaaatgcag
      901 cggcatcaaa taaagtacat acatctgaac catctgaatt cttcacaaaa ataccttttt
      961 caggctgctc cagtgtaatt ggacattgaa gcacctcacc aggacagtga agtttttcaa
     1021 tatcaacagt aaaggtacat tccgatattt tggcctgcaa agagttctct tgtcgctgcg
     1081 ccaccattcc aggaagcacg cttaacaccc tgtttctgag cgcatcaact ccatcattaa
     1141 aaccaatatt caataattct gcataaacca gtcccagccc ttcactttct gctaaaaaac
     1201 ggcctgtatc tggcatataa gaaactctaa ctctctcccc tcccaggaga aatcctccaa
     1261 tctgcccttc cattacctgc tgacgcattt ccgccatctc attcgcgaat gaagtcgatg
     1321 atatatatga tactaatcca ggcattacca tattccttca tacagataaa tatttcctct
     1381 gggcttcata acaaacatct ccctgacatg acaacaaaaa ccggagccgg actccggttt
     1441 ttgtgaatcc gtcggctatt tcatcccgcc aatatttccc cgtcagcacg ccagatttgc
     1501 agcggcctca ccactcactg tatatgcttt ttagctgcat ccagtacacc aattaccaca
     1561 tccttatacc ctgaacatct gtagcatttc atataagcaa ctggcagtta acatatagct
     1621 tgggtaatat ttatttcact tatcctttat gacaaagtta cctttggttt ggtcataaat
     1681 acattgttct tgacttacaa tcattgatga cgttattggt tctcgtgtta gcgggtgctt
     1741 cccaccgtcg cgaaccaaat gagaaaaaga aaccgaatca aataaggtac aaaccaaaga
     1801 accttctgaa tttttaacaa acacaccttc ttctggttta tccagtataa ttgggcatcg
     1861 aaccgcctcg gatggacact gaaggctctc cgtattaaca gaaaacttac attgtgatat
     1921 tttcccctca atatcaccat gtgattgtgt gttacctgaa cctgaaacca ttcttaacat
     1981 tatatccctg agcgcttcag gaccaccatt aagccctgta agtaataatc cagaaagcaa
     2041 actgttattg cccccactcg ccagaaagcg accatccgtt tcactatatg tgatacggac
     2101 cgtctgcccg cctactgtaa tttctccacc gccactggcg gccatttgag cacgaagaac
     2161 ctgcattccg gtggaaaatg aattggatgc aatagctgat gttaacggca tgacaaaact
     2221 ccctatttaa atattcattc caaacacagg gagtcataac aaacagcccc catacatgac
     2281 aacaaaaacc ggagccggcc tccggttttt tgtgaacccg tcggctattt catcccgcca
     2341 atattttccc acgtcccgtc agcacgcagg atttgcagcg gtcttaccac acactgtatc
     2401 tgctttttat ctgcatccag tatcaccacc tgtgtgatta ccctgtcctg ctccggaata
     2461 atgccattct catctgactc caggatgtct gccggcccca gtcgcagttg tactgtaagc
     2521 gactgcccgt gttcacagtc atcatgcttt ccgcaaccac acagacgctc cataagcttt
     2581 ctcaaaatat tcatgtcatt ctcctgttct gcctgtatca ctgcccactt catccagtcc
     2641 cttaacatcc tgccacggcc cgtcgccaaa cctgacctgc aaatgctgaa aaaaaccctg
     2701 aacccgtgtg gcatctttgg ggtcaagaaa ggtcagtccg gtgatgagtg cgccatctgt
     2761 atccgggaac cagccatggc tgtttgtctc aataatgctc gccggcccca gacgaaaacg
     2821 gatttgtgtc tcccccgggt cgcccttcgg tccctgaggt ccggttgccc ccaccgggcc
     2881 agccgcacct gtttctcctt tcggtccctg tgggccagcc gggcctgccg caccggtatc
     2941 tccctttgga ccctgtggac ctgcatttcc cgtcagaccg gtctctcccc gctctcccct
     3001 gtcgcctttc ggtccctg
//
LOCUS       DAZFXT010000253         2971 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.255, whole genome shotgun sequence.
ACCESSION   DAZFXT010000253 DAZFXT010000000
VERSION     DAZFXT010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2971
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.255"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..108)
                     /locus_tag="ACHKW0_004976"
     CDS             complement(<1..108)
                     /locus_tag="ACHKW0_004976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7477625.1"
                     /translation="MTNTAKILNFGRGNFAEQERNVADLDDGYARLSNML"
     gene            complement(141..437)
                     /locus_tag="ACHKW0_004977"
     CDS             complement(141..437)
                     /locus_tag="ACHKW0_004977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CII family transcriptional regulator"
                     /protein_id="HFO7477626.1"
                     /translation="MEQTSYSKLSQREIDRAETDLLINLSTLTQRGLAKMIGCHESKI
                     SRTDWRFIASVLCAFGMASDISPISRAFKYALDGITKKKSPVAAGDSKQIDMQF"
     gene            complement(576..809)
                     /locus_tag="ACHKW0_004978"
     CDS             complement(576..809)
                     /locus_tag="ACHKW0_004978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HFO7477627.1"
                     /translation="MSNELLRWRKEASSEEWKRLAALAKTSVGYLDQIAYGFRRASPD
                     KANAIEEATRNFTGYKPVKKENLVFVSRRASAA"
     gene            923..1627
                     /locus_tag="ACHKW0_004979"
     CDS             923..1627
                     /locus_tag="ACHKW0_004979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7477628.1"
                     /translation="MENKDIRKSNLAFLLDEHKKIAGNTNASFADKLGVSPSQLTQVS
                     GEKSTRNIGDKLARKFEAALGLPNGWLDLVHDVTPIASCSDSLTFVGQVRKGLVRVVG
                     EAILGVDGAIEMTEERDGWLKIYSDDPDAFGLRVKGDSMWPRIKSGEYVLIEPNTKVF
                     PGDEVFVRTVEGHNMIKVLGYDRDGEYQFTSINQDHRPITLPYHQVAKVEYVAGILKQ
                     SRHLDDIEAREWLKSS"
     gene            1688..2029
                     /locus_tag="ACHKW0_004980"
     CDS             1688..2029
                     /locus_tag="ACHKW0_004980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3024 domain-containing protein"
                     /protein_id="HFO7477629.1"
                     /translation="MAFNDLEYQAVKKEVHQFIESIRPPEHIRNELDIVYSINDQTID
                     IGEQRPVWQGNPGETNILPSARIKYIRSLDRWKIYWMRKDMKWHQYSTELSLTDALEL
                     VRANPDCCFFG"
     gene            2097..2558
                     /locus_tag="ACHKW0_004981"
     CDS             2097..2558
                     /locus_tag="ACHKW0_004981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001221210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477630.1"
                     /translation="MRILGVRAAPKVTSFVVYCTNESALKCVDVIKIPSTLDTPEKLK
                     YVRNNILDILNLYNVELAAIRVTESNSDNLSIDRLYIEAVIQEAFSSSDVRKYYTIRK
                     SGMKSSLNLTEIEYKEILKSHRNINGIDNSGFTTETNEAVLAALSAEVRGC"
     gene            2552..>2971
                     /locus_tag="ACHKW0_004982"
     CDS             2552..>2971
                     /locus_tag="ACHKW0_004982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO7477631.1"
                     /translation="MLTPYKRADVEFEWISDLEEQGCFSKVYLAHDRHLAHDLVIKEI
                     EKKENTNHDDYFNEARLLYKHAHPNIVQVQYAAQCESNIYIAMPFYHNGSLNQLMKKN
                     NLTSREIIRYSIQFLSGLYHIHSKGLMHFDIKPNNIMI"
BASE COUNT          916 a          565 c          649 g          841 t
ORIGIN      
        1 cagcatattt gatagtctgg cgtaaccatc atcgagatct gccacattac gctcctgttc
       61 ggcaaagtta cctctgccga agttgagtat ttttgctgta tttgtcataa tgactcctgt
      121 ggattgatcc agtaattccc tcagaattgc atatcaattt gcttagagtc cccggcggcc
      181 accggggatt ttttctttgt gattccatca agcgcatact taaaagccct actaatcgga
      241 ctgatgtctg atgccattcc gaaagcacac aagaccgaag caataaatct ccagtccgtt
      301 ctgcttatct tcgattcatg acagccaatc atctttgcca gaccgcgctg ggtaagcgtt
      361 gacaggttga tgagtaaatc agtttcagcg cgatcaattt ctcgctgtga tagtttgctg
      421 taacttgttt gttccatttc ttaagatttc caatagtgaa tagttagttg aaaggtatgc
      481 gtggaaacgc atgtggcctt agttggtcag atatattggg actcgctttg tcagcgacgt
      541 aggacgaatg tccattgtga aaatagcggt gttacttatg cagccgatgc tctacgcgat
      601 acgaacacta ggttttcctt tttcacaggt ttataacccg tgaaattacg agtagcttct
      661 tcgattgcat tcgctttatc aggggaagct cttcgaaatc catatgcaat ctggtcaaga
      721 tagccaactg aagttttcgc taatgcggcg agtcgcttcc attcctcact agaagcctct
      781 tttcgccagc gtagtagttc attactcatt agtgcctccg tttatcacac agaacaaatt
      841 taccattttg ataaatcatc cgcaatgtaa atttatcata ttgcgtattt atccatttgc
      901 taaatagagg gaaaattgtg agatggaaaa caaagatatt cgcaaatcga atctggcgtt
      961 tttgctagat gagcataaaa aaatcgcggg taacactaat gcaagctttg ccgataagct
     1021 tggggttagc ccttctcaac tcacgcaagt ctccggtgaa aaaagcactc gaaacatagg
     1081 ggataaacta gcaagaaaat ttgaagccgc gcttgggtta cctaatgggt ggcttgattt
     1141 ggtacatgat gtaacaccaa ttgcatcatg ctcagattct ttaacttttg tcggtcaggt
     1201 aagaaaaggg ttagtgcgcg tggttggtga ggcaattctt ggtgttgatg gtgccatcga
     1261 gatgaccgaa gagcgcgatg ggtggctcaa aatttatagc gatgatccag atgcctttgg
     1321 tcttcgtgtg aaaggagaca gcatgtggcc cagaataaaa tcaggagaat atgtactcat
     1381 tgagcctaac accaaagtat tcccgggtga tgaggtgttt gtcagaaccg ttgaaggaca
     1441 caacatgatt aaggttcttg gctatgacag agatggagaa taccaattta caagcattaa
     1501 ccaggatcac aggcctataa cgttgcctta tcatcaagta gcaaaggtgg agtatgtagc
     1561 tggtattctg aagcaatctc gccatctgga tgacatcgag gcaagggagt ggctgaaaag
     1621 ttcgtgactt catcgtcaca tagctggtaa ccagtggcct gaagagacgt ttgggtaagg
     1681 aggatagatg gcgttcaatg accttgaata tcaagcagta aaaaaagaag ttcaccaatt
     1741 cattgaaagc ataaggccgc ctgaacatat ccgcaatgaa ctggatattg tttatagcat
     1801 caatgaccaa acgatagata tcggcgaaca gcgccccgtg tggcagggca atccaggtga
     1861 aacaaacatc ctgccatcag caagaatcaa gtacatacgt tctctggata gatggaaaat
     1921 ctattggatg cggaaggata tgaaatggca tcagtacagt actgaacttt cgctgactga
     1981 tgcgcttgag cttgtgcgtg ctaacccgga ttgctgcttc ttcggatgag tgaagagacg
     2041 tttggatgat ggatggtcgc agagatgcgg cctgattcta aaataggata taaaaaatga
     2101 gaatactagg tgttagagcg gcgcccaaag ttacatcttt tgttgtatat tgcactaatg
     2161 agtctgcact caaatgtgtt gatgttatta aaataccttc gaccttagac acaccagaaa
     2221 aattaaagta tgtgagaaat aacatcctcg acattcttaa tttatataat gttgaattag
     2281 ctgccatacg cgttactgaa tcaaactctg ataatcttag cattgaccgc ctttatatag
     2341 aagctgttat tcaagaagca ttttcaagca gtgatgtaag aaaatattac actattagaa
     2401 aatctggcat gaaatcatca ttgaacctaa cagagatcga gtataaagaa atattgaagt
     2461 cacaccgcaa tataaatgga atcgataatt ctggttttac aactgaaaca aatgaagctg
     2521 ttttggctgc actatctgcg gaggtaaggg gatgctaact ccatacaaaa gagctgatgt
     2581 agaattcgaa tggattagtg atctagaaga acagggttgt ttttcaaaag tatatctggc
     2641 tcatgacaga cacctagctc atgacttggt gattaaagaa atagaaaaaa aagaaaacac
     2701 taaccacgac gactacttta atgaagcaag gcttctctat aaacatgcac atccaaatat
     2761 tgtgcaagtt cagtatgctg ctcaatgtga gagcaatatc tatatagcca tgccatttta
     2821 tcataatggt tcgctaaacc aattaatgaa aaaaaataat cttacaagca gggagataat
     2881 acggtattcc attcaatttt taagtggact ttatcatata cactcaaaag gtcttatgca
     2941 ttttgatata aaacctaata acattatgat a
//
LOCUS       DAZFXT010000254         2960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.256, whole genome shotgun sequence.
ACCESSION   DAZFXT010000254 DAZFXT010000000
VERSION     DAZFXT010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.256"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(346..711)
                     /locus_tag="ACHKW0_004983"
     CDS             complement(346..711)
                     /locus_tag="ACHKW0_004983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7477632.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNVLKGEQRRVSGSARVSLKRRIWLR
                     DAGQCCLCGRVVDLCDSELEHRIALQFGGGNEETNLWTLCTECHRQKSAREAASGMPD
                     PTLPEVSGGSGRADDIIGL"
     gene            753..938
                     /locus_tag="ACHKW0_004984"
     CDS             753..938
                     /locus_tag="ACHKW0_004984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7477633.1"
                     /translation="MSSKNRTRRTTIRNIRFPNHMIEQINIALEHKGSGNFSAWVIEA
                     CRRRLSTERSGMNYIIK"
     gene            complement(1068..1208)
                     /locus_tag="ACHKW0_004985"
     CDS             complement(1068..1208)
                     /locus_tag="ACHKW0_004985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477634.1"
                     /translation="MDFMLTVSGVVILSIAYTADKYGCHLLSRIGAYCSLMLIFSSLF
                     FE"
     gene            complement(1565..1789)
                     /locus_tag="ACHKW0_004986"
     CDS             complement(1565..1789)
                     /locus_tag="ACHKW0_004986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477635.1"
                     /translation="MKKLLVTVKPFQGTIPFRILQRGRVLVEGSFSGKCTQLHSRTFQ
                     VNATNEELTVECTMNAAKCRMVSAALQPVC"
     gene            complement(1854..2060)
                     /locus_tag="ACHKW0_004987"
     CDS             complement(1854..2060)
                     /locus_tag="ACHKW0_004987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rz1 family lipoprotein"
                     /protein_id="HFO7477636.1"
                     /translation="MRELKMKLCVLMLPLVVSACGSTPPAPVPCVKPPAPPAWAMMPP
                     SNSLRLLDETFSVSETESSATRQH"
     gene            complement(2283..2414)
                     /locus_tag="ACHKW0_004988"
     CDS             complement(2283..2414)
                     /locus_tag="ACHKW0_004988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477637.1"
                     /translation="MSDKLIMLAKILCVIVGISFSLMLVALFLSMGWMMLSSSGLLG"
     gene            complement(2707..>2960)
                     /locus_tag="ACHKW0_004989"
     CDS             complement(2707..>2960)
                     /locus_tag="ACHKW0_004989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7477638.1"
                     /translation="VPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEA
                     IRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWGIDR"
BASE COUNT          817 a          795 c          638 g          710 t
ORIGIN      
        1 ttgtttttcc cggacatgag cgccagcgca aattttcgtt tttgcgttgt cagcacagcc
       61 tcctcccggg tccagaacgc actcagccgg gtatggttca gcccattttt cccggcgtct
      121 catgccgcaa atgttaactg ctgcctggtt aacatttgct gaaaaagcca gttaacattt
      181 tttccgcaca acaaactgaa taataaagat aaaaaccgca aaaatgcccg gacagccagt
      241 taacatgtta actgccctga aacgggaatt ttttctctgc gtgagagggg gggcggtgtc
      301 caaagcgatc tttttttacg ccggatgata cccccccggg tcgggttaca gtccgatgat
      361 gtcgtccgct ctgccactac ctccggacac ctccggcagc gtcgggtctg gcataccact
      421 cgccgcttca cgagcagact tttgtcgatg gcattcggta cagagcgtcc agagattcgt
      481 ctcctcatta ccaccaccga actgaagtgc aatgcggtgc tcaagttcac tgtcacaaag
      541 gtcaaccaca cgcccacaga gacagcactg tccggcatcc ctcagccaga tacgacgctt
      601 gagggaaacc cgggcactac cactgacccg acgctgttca cccttcagga cattcacccg
      661 ccgggtgttc agagttttga ttctgcccgg taacgtacga agcacagcca tgtaaaatcc
      721 tctccatata gcttgtcacc agaggaaaga aaatgtcatc gaaaaaccgg acccgcagaa
      781 ccacaatccg caatatccgt ttccccaatc acatgattga acagatcaat atcgcccttg
      841 aacataaagg ttccggtaac ttttcagcgt gggttattga agcctgcaga agaaggctgt
      901 caacagagcg ttcgggtatg aattacataa ttaagtaaca tggtgttcac agaacacaca
      961 gttaccggac acatcagttt tccattcgtc ccccggcagt acaggcttcc cgtctgacgg
     1021 gatagcctga aaaaacacag aaaattattt gttataatta atataactta ctcaaaaaaa
     1081 agcgacgaga aaatcagcat caacgagcaa taagcgccaa tacgtgataa caaatggcag
     1141 ccatatttat ctgcagtata agcaatggac aggataacca caccagaaac cgtcagcata
     1201 aaatccattt gaacttcccc ggacaaaatc gactcatcta aagatttaca gctcttttta
     1261 ttatcaatat gttaaaagta aaataaacag atgttcaata atacgaatac aaaaacgtgc
     1321 tgaaaatcaa tgaatccatt tctgtgttat caattaatag tgataaacat ccggcttctt
     1381 ccaccatcgc accggaccag cgaccatgag gggacaacgc cgcgctccgt taacgcggta
     1441 aaccccggtg tgtatcgttt ttgattatcc ccgcacactc gcgcagagga ttctcccggt
     1501 cgggctgcgg tctctgttaa tgcaggaata cggcgacaat accgcgcatg aataataagg
     1561 tcgctcaaca cactggctgt aatgcagcgg ataccatgcg gcatttagcg gcattcatcg
     1621 tacactcaac ggttagctct tcattcgtgg cattcacctg aaaggtccgg gagtgtaatt
     1681 gcgtacattt accactgaac gaaccttcaa caagaacacg accacgctgc aaaatacgga
     1741 acggaattgt tccctgaaaa ggctttacgg ttaccaataa tttcttcatg cattctccga
     1801 ataacaaaaa tactagttaa tacactgagt gcggatatat tcctgaagca ttctcaatgc
     1861 tgcctggtcg ctgatgattc cgtctctgat accgagaacg tttcgtccag caaccggaga
     1921 gagttcgacg gcggcatcat tgcccacgcc ggaggtgccg gtggcttcac gcacggtacc
     1981 ggggcaggtg gcgttgatcc gcaggcgctt acgaccagcg gcaacatcag cacgcagagt
     2041 ttcattttca gctctcgcat cggctaattc cctcgagtat tttgcatcga gcgcagcaac
     2101 atcgcgctgg cgcacctgca tatcagtaat ggttgcgttt gccagatcca gctcactggc
     2161 ttttttatcg cgctgcgctt tgtaggtgat ggcgttatcg cggtaatgat tcagccccag
     2221 actaagcgca ccacagacca ccagcagaat aacggtaaac gcggaaagca ttcggtttat
     2281 gctcacccca gcagccccga cgaagacaac atcatccagc ccatggaaag aaaaagagca
     2341 accagcatta gtgaaaatga aatgccgacg attacacaga ggatcttcgc cagcattatg
     2401 agtttgtctg acatagctac cccttaattg ccacaattca ctgggatact acccataaaa
     2461 aagggatgct ccagaccagc aaaaatttcc agtttggtaa ttgactaatc atgagtcgca
     2521 actccctaat cagtttgcta aaatcaatca aggcagcctc ccatagctta ctgccataaa
     2581 cataaaaccc cgtttgcaac caacaaacgg ggtttttact tttattcact tgggttttgc
     2641 cagttcgcag gatttcgtgt tatctgcccg cgttggccaa cgtcattttt aagcaaaata
     2701 ttctgcttat ctgtcgatac cccagcacgc cagcgcactt tcctgatctc gccgggatac
     2761 ctgaccgtag caattatttg agcggatacg gcagtctctg ccaccgtcct taatccacca
     2821 gcgaatcgct tcgcaggcac cttttcgatc acctgcatta attcgtttat aaaacgtcga
     2881 cgggaaacac ttaccggggc caatgttata cggacagaat gacgcgatcc ccgctttctg
     2941 gggttcggtc agcggcactt
//
LOCUS       DAZFXT010000255         2923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.257, whole genome shotgun sequence.
ACCESSION   DAZFXT010000255 DAZFXT010000000
VERSION     DAZFXT010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.257"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            353..1330
                     /locus_tag="ACHKW0_004990"
                     /pseudo
     CDS             353..1330
                     /locus_tag="ACHKW0_004990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041206064.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            1849..>2923
                     /locus_tag="ACHKW0_004991"
     CDS             1849..>2923
                     /locus_tag="ACHKW0_004991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcdB toxin N-terminal helical domain-containing
                     protein"
                     /protein_id="HFO7477639.1"
                     /translation="MRLPEKVLFPPVTSGLSGQEKQKKPKSITGFQENYQRNIRPIKT
                     ASEARLRFFDKMVSKENSLEDVVSLGEMIQKEIYGHKQRTFSPVHHTGNWKSSLLHNA
                     LLGLANVYNGLRETEYPNTFNRDGIKSTNSFRDNSLTKTRTPRDNFEEGIKHPEHATI
                     PYDNDNESNKLLKAGKIAGNNNELLMEIKKESQSDHQIPLSDKFLKRKKRSPVAEDKV
                     QNSLTPENFVQKISLSDELKTKYANEIIEIKRIMGEYNLLPDKNSRNGLKLLQKQADL
                     LKIIMEDTSVTENTFKNIEMAITDIKREYYSHTVDIEKNIHAIWVAGSPPESISDYIK
                     TFLKTYKEFTYYLWVDEKAFGAAK"
BASE COUNT          981 a          592 c          608 g          742 t
ORIGIN      
        1 ggctaaaatt aattctattt cattatatga gaacaccaat aagaataata atatttgatc
       61 acaactgaag atgcttttta caatgaagaa ttattgttaa aaaacgcttt gttgaatcaa
      121 ccgaactgtt gctgagttgc aggatcacat cacgtatttt cctgaaaacg caggctgctt
      181 agtagcaaag cagaagttca aaatcaccaa ctaaatagct gcgccgaata gtagatcaat
      241 gaaagggaac tcagcccgga ttgtgcgatc tgatcaatcg ccaaaaatcc ccccctaacc
      301 ggactgagcg atgccgatca tagcaccaat tccccgcgac gaatgacgcc tgatgcaaaa
      361 agcaatctat aaaactcacg ataaaaatta tgcccgcagg ctgaccgcca tgctgatgct
      421 gcaccggagc gctcgcgtca gtgacgttgc cagaacgctc tgctgcgccc gttcctccgt
      481 caggcgctgg attaactggt tcacgctgtc gagtgttgaa ggtctgaaat cattacccgc
      541 aggacgttcc cgccgctggc cgtttgagca tatctgcaca ttgtttcgtg aactgataaa
      601 acactctccc ggatattttg gctatcagcg ttcacgctgg agtacagaac tgctggcaat
      661 aaaaatcaac gagataaccg agtgccagtt acatgccgga accgttcgcc actggttgcc
      721 attagtcggg cttgtgtggc gcagggctgc gccaaccctg cgtacccgta acccgcataa
      781 agcagcaatt cacaaagcac tggacgaatg tagtgcggag catccggtct tttatgaaga
      841 tgaagtgtat atctatctta atcccaaaac cggtgcggac tggcaacttc gcagacagca
      901 aaaacatgtg gtaacaccgg ggcagaatga aaaatactat ctggctggtg ctttacatag
      961 tggaacgggc aaagtcagct atgtgggtgg caacagcaaa agctcagcct tatttatcag
     1021 tctgttgaaa catctgaaat tgacataccg acgggataaa cctatcacgc tcatagtgga
     1081 caactacatc atccataaaa gtcgggaaac acagcgctgg ctgaaagaaa atccgaagtt
     1141 cagagtgatt tatcagccag tttactcgcc gtgggtgaat catgttgagc ggttatagca
     1201 ggcgcttcac gatacgataa cgcgcaatca tcagtgccgt tcaatgtggt aactgcttaa
     1261 aaaggttcat tattttatgg aaaccgtgag cccattcccc ggtggtaaat atgggctgtt
     1321 aaaagtgtag cggtattcgg cgcagctatt taggatggtc aggtcgcaga agttctgtgc
     1381 cactacagtg cgtgtactaa acaaaatgac gagggcacga atgtcataat acgtgcgtat
     1441 tacctgcagt agctaccaac tgagggggct tatcccaaac ctgatttatt caacaaagct
     1501 ggacttatcc ttactctgaa ttattttcca gacatatgca tcatgtactg gctcgcttgg
     1561 ttcaggaaat acaggagcaa atctacagct attagattta ttcctatttg tatgcaaagt
     1621 cgcattgcat aatgcggagc tatgttacag atacgcctga cgtgcgtagt aaaattataa
     1681 agcaacatat atatacaagg agaaaccgag aaaaataaat gagtcgtatg ttgggtagca
     1741 tcgagataaa tatttttttt atcacattag caccttggtt aagggtgtta ataataatat
     1801 tctctcactt attaaaacat aaataatttt ttaagcggaa atgtaattat gagactgcca
     1861 gagaaagttc tttttcctcc tgtcactagt ggcctgtcag ggcaggaaaa acaaaaaaaa
     1921 ccgaagagca ttaccggatt tcaggaaaat tatcaacgca atatcaggcc aatcaaaaca
     1981 gcatcagaag cccgactacg cttctttgat aaaatggttt cgaaagaaaa ctctctggaa
     2041 gatgttgttt ctttaggtga aatgattcag aaggaaattt atgggcataa acaaagaaca
     2101 ttttcaccag ttcatcatac aggtaactgg aaatcatcat tgttacacaa cgcgctcctt
     2161 ggtctggcaa atgtttataa tggcttacgg gaaacagaat accctaacac tttcaacaga
     2221 gatggtataa aaagtactaa ctcttttaga gataactcat tgacaaaaac aagaactccc
     2281 agagataatt ttgaggaagg aataaaacat cctgaacatg caacaatacc atatgacaac
     2341 gacaatgaaa gtaataaatt gctaaaagca ggaaagatag ctggtaacaa taacgagctg
     2401 ttgatggaaa taaaaaagga atcccaaagc gaccatcaaa tccccctgtc agataagttc
     2461 ctgaaaagga aaaaacgatc tcctgtagct gaagataaag ttcaaaactc gttaacacca
     2521 gaaaattttg ttcagaaaat ttcacttagt gatgagctta aaacaaaata tgcaaatgaa
     2581 attatagaga taaaaagaat aatgggagaa tacaatcttt taccggataa aaacagtcgt
     2641 aatggtctaa aacttctaca gaagcaagct gatttactaa aaataatcat ggaggatacc
     2701 tctgttacag aaaatacctt taaaaacata gagatggcta taacagatat aaaaagggag
     2761 tactattcgc atacagttga tattgagaaa aatattcatg ccatatgggt tgcgggttcc
     2821 ccacctgaaa gcatctcgga ctatattaag acttttctca aaacctacaa agagtttact
     2881 tactatcttt gggttgatga aaaagcattt ggagccgcga aat
//
LOCUS       DAZFXT010000256         2911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.258, whole genome shotgun sequence.
ACCESSION   DAZFXT010000256 DAZFXT010000000
VERSION     DAZFXT010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.258"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..108)
                     /locus_tag="ACHKW0_004992"
     CDS             complement(<1..108)
                     /locus_tag="ACHKW0_004992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HFO7477640.1"
                     /translation="MTNTAKILNFGRGNFAGQERNVADLDDGYARLSNML"
     gene            complement(141..434)
                     /locus_tag="ACHKW0_004993"
     CDS             complement(141..434)
                     /locus_tag="ACHKW0_004993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HFO7477641.1"
                     /translation="MVRANKRNEALRIESALLNKIAMLGTEKTAEAVGVDKSQISRWK
                     RDWIPKFSMLLAVLEWGVVDDDMARLARQVASILTNKKRPAATERSEQIQMEF"
     gene            complement(554..769)
                     /locus_tag="ACHKW0_004994"
     CDS             complement(554..769)
                     /locus_tag="ACHKW0_004994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HFO7477642.1"
                     /translation="MQTLSERLKKRRIALKMTQTELATKAGVKQQSIQLIEAGVTKRP
                     RFLFEIAMALNCDPVWLQYGTKRGKAA"
     gene            873..1505
                     /locus_tag="ACHKW0_004995"
     CDS             873..1505
                     /locus_tag="ACHKW0_004995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7477643.1"
                     /translation="MSISSRVKSKRIQLGLNQAELAQKVGTTQQSIEQLENGKTKRPR
                     FLPELASALGVSVDWLLNGTSDSNVRFVGHVEPKGKYPLISMVRAGSWCEACEPYDIK
                     DIDEWYDSDVNLLGNGFWLKVEGDSMTSPVGQSIPEGHMVLVDTGREPVNGSLVVAKL
                     TDANEATFKKLVIDGGQKYLKGLNPSWPMTPINGNCKIIGVVVEARVKFV"
     gene            1595..1906
                     /locus_tag="ACHKW0_004996"
     CDS             1595..1906
                     /locus_tag="ACHKW0_004996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477644.1"
                     /translation="MNGDRYEFTKDSTEDSVFNVTINGDKSSVYESVSGVYPEMKYTA
                     LSSNTMVGEYQSGGGITVETWSITTDKKALYSKVMNIPGMQQLTSTKSFVGDVVGTCN
                     Q"
     gene            2126..2581
                     /locus_tag="ACHKW0_004997"
     CDS             2126..2581
                     /locus_tag="ACHKW0_004997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein N"
                     /protein_id="HFO7477645.1"
                     /translation="MCDTKRSIGRKCESGLAANVPLRGVFVQDYDSHTQPKLTDRRIQ
                     MDAQTRRRERRAEKQAQWKAANPLLVGVSAKPVNRPILSLNRKPKSRVESALNPIDLT
                     VLAEYHEQIESNLQRIERKNHRVWYSKPGEFGITCQGRQKVKGESIPLA"
     gene            2590..>2911
                     /locus_tag="ACHKW0_004998"
     CDS             2590..>2911
                     /locus_tag="ACHKW0_004998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477646.1"
                     /translation="MKKIDYKSIPKPIDSASERKKHKKEAEKLANYISFIRNNAHGDG
                     DKKLLSDARTQAFGILRKQMQYRLHPGYIIEIRPTERQLFLLNSVFDFVNVVGDLIDR
                     SVDKD"
BASE COUNT          848 a          629 c          653 g          781 t
ORIGIN      
        1 cagcatattt gatagtctgg cgtaaccatc atcgagatct gccacattac gctcctgtcc
       61 ggcaaagtta cctctgccga agttgagtat ttttgctgta tttgtcataa tgactcctgt
      121 tgatagttcc agtaataacc tcagaattcc atctggattt gttcagaacg ctcggttgcc
      181 gccgggcgtt ttttattggt gagaatcgaa gcaacttgtc gtgccaatcg agccatgtcg
      241 tcgtcgacga caccccattc aagaacagca agcagcattg agaactttgg aatccaatcc
      301 ctcttccacc tgctgatctg cgacttatca actcccacag cttccgctgt cttctcagtt
      361 ccaagcattg cgattttgtt aagcaacgca ctctcgattc gtagagcctc gttgcgtttg
      421 tttgcacgaa ccatatgtaa gtatttcctt agataacaat tgattgaatg tatgcaaata
      481 aatgcataca ccataggtgt ggtttatttt gatgcccttt ttcagggctg ggatgtgtaa
      541 gagcgggaat gtcttaagcg gctttaccgc gtttagttcc gtactgtaac caaaccggat
      601 cacagttaag cgccatagca atctcaaaca agaagcgcgg tcgcttggtt actccagctt
      661 caatcagttg aattgattgc tgtttaacac cggctttggt tgccagttcg gtttgcgtca
      721 tttttaacgc aattcgcctc ttcttgaggc gttcagaaag agtttgcata tcgcctccat
      781 caacaaactt tcttgtattt tcatacaatg tatcttgttt gtcaaataca gtttttcttg
      841 tgaagattgg gggtaaataa cagaggtggc ttatgagtat ttcttccagg gtaaaaagca
      901 aaagaattca gcttggactt aaccaggctg aacttgctca aaaggtgggg actacccagc
      961 agtctataga gcagctcgaa aacggtaaaa ctaagcgacc acgcttttta ccagaacttg
     1021 cgtcagctct tggcgtaagt gttgactggc tgctcaatgg cacctctgat tcgaatgtta
     1081 gatttgttgg gcacgttgag cccaaaggga aatatccatt gattagcatg gttagagctg
     1141 gttcgtggtg tgaagcttgt gaaccctacg atatcaagga cattgatgaa tggtatgaca
     1201 gtgacgttaa cttattaggc aatggattct ggctgaaggt tgaaggtgat tccatgacct
     1261 cacctgtagg tcaaagcatc cctgaaggtc atatggtgtt agtagatact ggacgcgagc
     1321 cagtgaatgg aagccttgtt gtagccaaac tgactgacgc gaacgaagca acattcaaga
     1381 aactggtcat agatggcggt cagaagtacc tgaaaggcct gaatccttca tggcctatga
     1441 ctcctatcaa cgggaactgc aagattatcg gtgttgtcgt ggaagcgagg gtaaaattcg
     1501 tatgatcagg attgcggcgc tactctcaat actcttaact accagcgcca attctgaatg
     1561 ctggattgtc acaaacctgc acgggtacgg ggcaatgaat ggcgatcgtt acgagtttac
     1621 aaaagacagc acggaagatt ccgttttcaa cgtaacaata aatggcgata aatcatcagt
     1681 ttatgaatca gtttctggcg tctatccaga gatgaaatac actgctttgt catcgaacac
     1741 tatggtagga gaataccagt ctggaggagg cataaccgtt gaaacttggt caatcactac
     1801 agacaaaaaa gctctttact ccaaagtaat gaacatccca ggtatgcaac aacttacatc
     1861 aaccaaatcc tttgttggtg atgtagtcgg aacctgcaat cagtaatccc cacctcaatc
     1921 tcgataacaa aaaaacaaac tattttccgt ttaaaaacaa tggagtttgt ttttcacgcc
     1981 cttttttaca atatttcttg tttacaacat acaatcttcc ttgtaatttt aagccatcag
     2041 caggacgcac tgaccaccat tgaaggtgag gctcttaaaa attaagccct gaagaagggc
     2101 agcattcaaa gcagaaggct ttggtgtgtg tgatacgaaa cgaagcattg gccggaagtg
     2161 cgaatccgga ttagctgcca atgtgccatt gcggggtgtt ttcgttcagg actacgactc
     2221 ccacacacaa ccaaagctaa ctgacaggag aatccagatg gatgcacaaa cacgccgccg
     2281 cgaacgtcgc gcagagaaac aggctcaatg gaaagcagca aatcccctgt tggttggggt
     2341 aagcgctaaa ccagttaacc gccctattct ctcgctgaat cgcaaaccga aatcacgagt
     2401 agaaagcgca ctgaatccga tagaccttac ggtgctggct gaataccacg aacagattga
     2461 aagcaacctg caacgtattg agcgcaagaa tcatcgagtt tggtatagca agccaggtga
     2521 gttcggtata acttgtcaag gaagacaaaa ggttaaaggg gaatccattc cattggcatg
     2581 aggtacgtaa tgaagaagat tgattacaag tccataccaa aaccaataga ctcagcatca
     2641 gagcgaaaaa aacacaaaaa agaggctgaa aaattagcaa attatatcag ttttattaga
     2701 aacaatgctc acggcgatgg cgacaagaag ttgctttcag atgctcggac ccaagcgttc
     2761 ggtatacttc gtaagcagat gcaatatcgt cttcatcctg gctacataat tgaaattcgc
     2821 cctactgaaa gacaattgtt cttattaaac tctgtctttg actttgtaaa cgtagttgga
     2881 gatcttatcg acaggtctgt agataaggat c
//
LOCUS       DAZFXT010000257         2911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.259, whole genome shotgun sequence.
ACCESSION   DAZFXT010000257 DAZFXT010000000
VERSION     DAZFXT010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.259"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(535..708)
                     /locus_tag="ACHKW0_004999"
     CDS             complement(535..708)
                     /locus_tag="ACHKW0_004999"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477647.1"
                     /translation="MNKTANVAAHAVVNAALAVVQGNNARASAAGAATGDVVGMIATE
                     MCGISPGTVTCKR"
     gene            886..1089
                     /locus_tag="ACHKW0_005000"
     CDS             886..1089
                     /locus_tag="ACHKW0_005000"
                     /inference="COORDINATES: protein motif:HMM:NF026057.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase DNA-binding-containing protein"
                     /protein_id="HFO7477648.1"
                     /translation="MVLSDCYSLANDQSGHARLGDPRRTRRLVSLTSSLAQHAGLSIV
                     KSSHFTAQVEGAYRLIRNPSVSP"
     gene            complement(1021..1254)
                     /locus_tag="ACHKW0_005001"
     CDS             complement(1021..1254)
                     /locus_tag="ACHKW0_005001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477649.1"
                     /translation="MSQQPVRLRVSGGIPQVIKCRTVTEVNGGGIFQREEQRMCLTCP
                     CSGSKSGFGNGLRGHRRVTYQAVRTFNLSSEVG"
     gene            1264..2223
                     /locus_tag="ACHKW0_005002"
     CDS             1264..2223
                     /locus_tag="ACHKW0_005002"
                     /inference="COORDINATES: protein motif:HMM:NF033590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HFO7477650.1"
                     /translation="MYAPDSALPVDLIEQQCWSRATDTYGVKHQRKERPCEEKESYRW
                     QQASERMAERLGEIQKQVITVCDREADIWHYLHYKVSRGKPHAECDAKRRAGSPPGPD
                     AHQLQRSQHKKSRQQRPGALAHVCLLRGAGEDGACWYPLTSEKVASAADARRIVSHYE
                     QRWLIEEYHKAWKSGGTCVESLRMQTRDNLERMVVIAVRVLGLRQGGISEETQNDSCE
                     KILTPTEWKLLWVKLEGKQLPAQTPTLKWACLKLAKLGRWHDSKRTSSPGWVVMWDGW
                     FRHQDMAEGYLVMKSLDQEICSRDRSKMGDNVWCCITAQASHS"
     gene            2388..2911
                     /locus_tag="ACHKW0_005003"
                     /pseudo
     CDS             2388..2911
                     /locus_tag="ACHKW0_005003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001178393.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          796 a          713 c          662 g          740 t
ORIGIN      
        1 cttcattaat aaatgatttc tgaaaatcaa aaaggaccac acatctcaaa aacattattt
       61 gaaatcatca tcaaaaaact ataaatatat cacctgacga tttgaacaca ctgacaaaaa
      121 accaagcatg gtaataaacc gaactcccca caaacttctg ttgcctcaga tactcccccc
      181 ctttcctcag gcacaaatgt tgttccataa cgaaccagcc ctgttccttt aaactctgca
      241 tcaatggcat aatgtatact caccagactg agtttgctcc tgcatcatta tttcgatacg
      301 ttaatgcatt gttcattgca tccggcattc cccatcgaat atttactttg ttatatcagt
      361 cagcccataa ccaggtaaat ttttaaggct tactgactga atattcaaaa tgtgttacat
      421 gttatcaaac ctttaaagaa taacctgctc ttcttcattg gccggattta gctcaatctt
      481 gtcagcaaaa ctaatataaa gccgttcata aagagaagga tgtagcttcg ttatttatct
      541 tttacatgta acagtgccag gggatattcc gcacatctct gtggctatca tccccaccac
      601 gtctccagtg gcagcacctg cggcactggc acgggcgtta tttccctgta ccaccgccag
      661 tgcagcattc accacggcat gggctgccac atttgcagtc ttattcacca cacctgtaac
      721 ctgtctctta tacacaaatc cccacccggg gatttgtgat gtctgtcagt ctgctttagg
      781 gggactcttt ccatccggta gtttccttcc tccccggcag cgattttttg aactttttct
      841 tcacccgttg atcttctctt atatttcatt ttcttcgggt ctgttatggt actttctgat
      901 tgttattcac tggctaacga tcagtctgga catgcccgtc tcggtgatcc gcgtcgtaca
      961 cgacgccttg tgtcactgac ctcttctctc gctcagcatg caggtctttc tattgtgaaa
     1021 tcatcccact tcactgctca ggttgaaggt gcgtaccgcc tgatacgtaa cccgtcggtg
     1081 tccccgtaag ccattgccga agccggattt actgccactg caagggcatg tgaggcacat
     1141 ccgctgctcc tcgcgctgga agataccacc accattaact tcggtcacag tacggcattt
     1201 gatgacctgg ggaataccac cactaacccg aagacgcacg ggctgctggc tcactctgtc
     1261 ctgatgtacg cccccgacag cgccctgcca gtcgatctta tcgaacagca gtgctggagt
     1321 cgtgccacgg acacctacgg tgttaagcac cagcgaaaag aacgtccctg tgaagaaaaa
     1381 gaaagttacc gctggcagca ggcttcggag cgcatggccg aacggctggg ggagatacag
     1441 aaacaggtaa tcactgtctg tgaccgggaa gcggatatct ggcattacct gcattataaa
     1501 gtcagccgcg ggaagccaca cgctgaatgt gatgcaaaaa ggcgggcggg cagcccgcca
     1561 ggcccggatg ctcatcagtt acagcgaagt cagcataaaa aatcccgaca acagcggcca
     1621 ggcgctctcg ctcacgtatg tctgttgcgg ggagcaggag aggacggagc ctgctggtat
     1681 ccgctgacat cagaaaaagt ggcgagtgcc gccgatgcgc gacgtatcgt cagccattac
     1741 gagcaacgct ggctgataga ggaataccac aaagcgtgga aaagtggtgg tacatgtgtg
     1801 gaatcgctgc gaatgcagac ccgggataac cttgagcgca tggtggttat cgcggtgcgg
     1861 gtgctgggcc tgcggcaggg aggtatcagt gaagaaacac aaaatgacag ctgtgagaaa
     1921 atactgacgc cgacagagtg gaaactactg tgggttaagc tggaaggaaa gcagcttccg
     1981 gcgcaaacac cgaccctgaa atgggcctgt ttgaaactgg ctaaactggg gcgatggcat
     2041 gacagcaaac gcacaagcag ccccggttgg gtagttatgt gggatggctg gttcagacat
     2101 caggatatgg ctgagggtta cctggtgatg aagtctcttg atcaggagat ctgctcaaga
     2161 gacaggtcaa agatggggga caacgtctgg tgctgtataa cagcacaggc ctcacactct
     2221 tgacgcccgt ggaatatatt agagaacaaa aatttcaata tgtggtggac gcatacagag
     2281 gattgacagc attcccatac taattgttaa gattcaaatt gaagtatatc tatctgtcat
     2341 tttgcaaatg atatgcggta gctttaccta actatcatga acatttcgaa aaatcatcca
     2401 caaagccccg aagaataaag cactatcgcc cgataaagac ctgtaagctc agtggcatgg
     2461 atactattga gctcagaatg gcgagctgta ccgttatttc attaccatgc tcgacgaaca
     2521 cagcaactac tctctggccg tactatcgct taaaagtgat atcatcaggc atttcttctg
     2581 ctgtgtagcc cagttgttcc ttgttggtat caaccagatc atcactgatg atgggcaaag
     2641 agttctcggg aaaattcgac aaaacgctac aggaagccac cgtcgggttc gtatggactt
     2701 atctctatac gccgaaaatt aaatgccata agtgagcatt ttaaccgaac actgagggaa
     2761 caatttattg agtttaatga agaattgctc tttgaatagc tgaatatgtt caatctgaaa
     2821 ctggctgagt atctggtact gtacaacagt aaaattcttc ataaatcgtt taaactacaa
     2881 aaatcagtag aatatattat ttccactgtc a
//
LOCUS       DAZFXT010000258         2900 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.260, whole genome shotgun sequence.
ACCESSION   DAZFXT010000258 DAZFXT010000000
VERSION     DAZFXT010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2900
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.260"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..135
                     /locus_tag="ACHKW0_005004"
     CDS             <1..135
                     /locus_tag="ACHKW0_005004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7477651.1"
                     /translation="ICNVTDAISLDTWKKTVKRFYDTLVVQFDIEEAWAEQQLKKVTR
                     "
     gene            362..559
                     /locus_tag="ACHKW0_005005"
     CDS             362..559
                     /locus_tag="ACHKW0_005005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7477652.1"
                     /translation="MLKQQDMTETARAVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQFGCYRRLQS"
     gene            710..1768
                     /locus_tag="ACHKW0_005006"
     CDS             710..1768
                     /locus_tag="ACHKW0_005006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HFO7477653.1"
                     /translation="MLNTVKISSCELVNADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYEAKGRALKQHVMA
                     PLISYFRDARAALGITAKQIVDATGKKNMVSHWFSASQWQLPNESDYLKLQALFARVA
                     EEKHQRGELEKPHHQLLETYTSLNRQYAELQSEYKHLRRYFGVTAQVPYTDVWTHKPV
                     QYYPGKHPCEKPAEMLQQIISASSRPGDLVADFFMGSGSTVKAAMALGRRATGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            2272..>2900
                     /locus_tag="ACHKW0_005007"
     CDS             2272..>2900
                     /locus_tag="ACHKW0_005007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7477654.1"
                     /translation="MTFKHYDVVRAASPSDLADALAQKIREGWQPYGGPFSSYTDDGA
                     ALIQAIVAEGDVSTPVVVKPTGGEGAVISATSDPEYYFVVVLAGQSNGMSYGEGLPLP
                     ETYDRPDPRIKQLARRSTVTPGGAACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQ
                     YGTVGQGLHIAKKLLPFIPANAGILLVPCCRGGSAFTTGADGTYSDASGA"
BASE COUNT          714 a          671 c          837 g          678 t
ORIGIN      
        1 atatgtaatg taaccgatgc catatctctt gatacatgga aaaaaacagt taaacgtttt
       61 tacgatacgc tggtggtgca gtttgatatt gaagaagcat gggcagaaca acaactgaaa
      121 aaggtgacca gatagctttg ttgatttttc ccgaatctgt ggtaaatttg ccctaacgat
      181 gggcgtttta tgcctgacgt tagaagattt tttacacccc gccgcctggc gggtttttta
      241 tgactgaaat cgcgtcagta cagtaaacgc gctggtggcg gtgaataccg gtctttcagc
      301 ttgctggctt ttttgacaag agttattggt gtgtcacgtt aaccgaaaaa gggaaaaaga
      361 catgctgaaa cagcaggata tgacagaaac tgccagagca gtgtttaatg aattaagcgt
      421 caccgaaccg gcgacagtcg gggagattgc gcagaatact tacctttcac gcgaacgctg
      481 ccagttaata ctgacccagc ttgttatggc gggtctggca gactatcagt tcggttgtta
      541 cagacgcctt cagtcctgaa ggctttttta tttgtggtaa atgggcgact ggtgggtgtt
      601 aggggcactc accagccatc tgctcatgcg tccggatcac aagcaaacct caggcccact
      661 gctttgcgca aaagcagaat gagcctatca gagacaggct taatgatcca tgcttaatac
      721 tgtaaaaata tccagctgtg agttagtcaa cgccgactgc ctggaattta tccggtcgtt
      781 acccgaaaat tctgttgacc tgatagtcac ggacccgccg tactttaaag tgaagcccga
      841 gggctgggat aaccagtgga agggcgacga tgattacctg aagtggctgg accagtgtct
      901 ggcgcagttc tggcgggtgc tgaaacctgc cggaagtctt tacctgttct gtggccatcg
      961 cctggcatct gatatcgaaa tcatgatgcg tgaacgcttc agtgtgctga accatattat
     1021 ctgggcgaag ccgtccggac gctggaacgg gtgcaacaag gaaagcctga gggcgtattt
     1081 ccccgccaca gagcgcattc tgttcgcgga acattatcag gggccgtatc gtccgaaaga
     1141 tgccgggtat gaggcgaagg gcagggcact gaaacagcat gtgatggctc cgctgatttc
     1201 ttactttcgt gatgcgcgtg ctgccctggg gataacggca aaacagatag tggatgccac
     1261 aggaaagaaa aacatggtgt cgcactggtt cagtgccagt cagtggcagc taccgaacga
     1321 aagcgattat ctgaaattac aggcgctgtt tgcccgggtg gcagaagaga agcatcagcg
     1381 gggtgaactg gagaagcccc accaccagct gctggagacg tatacttcac tgaaccggca
     1441 gtatgcggaa ctacagagtg aatataagca tctgcggcgg tattttggcg tgacggcgca
     1501 ggtgccgtac acggatgtgt ggacgcataa accggtgcag tactatcccg ggaaacatcc
     1561 gtgcgaaaaa ccggcagaaa tgctgcagca gataatcagt gcgagcagtc gtccgggtga
     1621 cctggttgca gatttcttca tggggtcggg ttcgacagtc aaagccgcga tggcgctggg
     1681 gcgtcgtgca actggcgttg agctggagac tgaacgtttt gagcagacgg tcagggaagt
     1741 acaggattta gtcagtcaga acggatgata ttgcagaatt aaccgggtat caggtacgcc
     1801 ccgaaaattt taaatgtctc acaattcaga cggttgacag ttgtctggtt tgcggggagt
     1861 ttgttaaaag aaactggcat ggtgaatccc cctgagcgga ggggcatatc agcgcaggtg
     1921 tttctactct atcctttctg tgcgggttca ggtgctgata ctgaactcac cgggaggcac
     1981 ccggcaccat gcatatggtt aacagacacg tagcgaagcc cctctccgga ggggcttttt
     2041 ttatgggcaa aaaaagcccg cgctgggaga cgcgggcggc aaggaataaa caacaaaacg
     2101 tgaagtaata tttcagctgg cgaataatac cccatggtaa tcactctgcg caactgcgcg
     2161 gcctttttcg aattgcgggc tgtaagtctc ccttctgcca ttgtcctgta acttccggac
     2221 ttcagcccgc tctttatctg attcagtaca ctatcccggc cgggaggatt catgacattt
     2281 aaacattacg atgtggtcag ggcggcatcg ccgtcagacc ttgctgatgc acttgcgcaa
     2341 aaaattcgtg aaggatggca accatacggt gggccgtttt cttcgtatac ggatgatggc
     2401 gcagcactta ttcaggcgat tgtcgcagaa ggtgatgtga gcacacctgt tgtggtgaag
     2461 ccgacaggtg gagaaggtgc agtaatcagt gccaccagcg acccggagta ttactttgtt
     2521 gtggttctgg cagggcagtc aaacggcatg tcgtatggtg aaggtcttcc gctgccggag
     2581 acatatgacc gtccggaccc gcgtattaag cagctggcgc gccgcagtac ggtgacaccg
     2641 ggcggtgcag catgcaaata taacgacatc attccggcgg accattgtct gcatgatgtg
     2701 caggacatga gccgccttaa ccatccgaaa gcggacctgt caaaggggca gtacggaacc
     2761 gtggggcagg ggctgcatat cgccaaaaaa ctgctgccgt ttataccggc gaatgcgggc
     2821 attctgctgg ttccgtgctg tcgtggtggt tcagcgttca ccaccggagc tgatggcaca
     2881 tacagtgacg cgagtggtgc
//
LOCUS       DAZFXT010000259         2868 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.261, whole genome shotgun sequence.
ACCESSION   DAZFXT010000259 DAZFXT010000000
VERSION     DAZFXT010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.261"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            269..994
                     /locus_tag="ACHKW0_005008"
     CDS             269..994
                     /locus_tag="ACHKW0_005008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7477655.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
     gene            952..1629
                     /gene="bglJ"
                     /locus_tag="ACHKW0_005009"
     CDS             952..1629
                     /gene="bglJ"
                     /locus_tag="ACHKW0_005009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HFO7477656.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGSPYKLHTYTSQ
                     ESFQDAMSRISFTAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCET
                     SNVLHPY"
     gene            complement(1667..2455)
                     /gene="fhuF"
                     /locus_tag="ACHKW0_005010"
     CDS             complement(1667..2455)
                     /gene="fhuF"
                     /locus_tag="ACHKW0_005010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HFO7477657.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            2596..2832
                     /locus_tag="ACHKW0_005011"
     CDS             2596..2832
                     /locus_tag="ACHKW0_005011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HFO7477658.1"
                     /translation="MLQRMLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAGIMLMIMNLNQG"
BASE COUNT          766 a          604 c          711 g          787 t
ORIGIN      
        1 aatgaatgtg atttctgttt ttcattaagt attcctaaca acttaatgta gatattttaa
       61 atgtctccag gctatttcac taggttatca tttgatgcta accttctgac acatgcagtg
      121 gagttgttgt gcagcaggag tatgctgata tgaaagtgaa tgctaaggat aatttattcg
      181 cttaatctat taatttgctg gaaacattta aggaatgaat atcctgaata aaccatcata
      241 atttttagac agtggatgtg gaggaaatat gttgccagga tgctgcaaaa atggaattgt
      301 tatcagtaaa atacctgtta tgcaagcagg gctaaaagag gtcatgagga ctcacttccc
      361 tgaatatgaa ataatatcca gcgcctctgc ggaggacctt accttattac aattacgtcg
      421 ttccggatta gtcattgctg atttagccgg tgaaagtgaa gatccacgtt ctgtttgtga
      481 acattattat tctttaatct cacaatatcg ggaaattcac tgggttttca tggtgtcacg
      541 ctcctggtat tcccaggcag tagaactgct catgtgccct acggcgacgt tattgtctga
      601 tgttgaaccc attgagaatc tggtcaagac cgtacgttcc ggcaatacgc acgcagagcg
      661 tatcagcgcc atgctgacct ctccggcaat gactgaaact catgatttta gctatcgctc
      721 cgtcattctc actctttcag agcgcaaggt actgcggcta ttaggtaaag gatggggcat
      781 caaccagata gcttcattgc ttaagaaaag taataaaact atcagcgccc aaaaaaacag
      841 tgcgatgcgt cgactggcaa ttcacagcaa cgctgaaatg tatgcatgga taaatagcgc
      901 gcagggtgca agagaactta acttgccttc tgtttatgga gatgccgcag aatggaacac
      961 agccgaatta agaagagaaa tgtcgcactc atagaaaaat gcgtcatgag tagtatcggt
     1021 attgagagtt tattcagaaa gtttgcgggt agcccttata agctccatac ctataccagt
     1081 caggagtcat ttcaggatgc catgtcgcgg atctcgttta cggcggtcat tttttctttt
     1141 tctgccatga gaagtgagcg cagagaggga ttatcttgcc tgactgaact ggcgattaag
     1201 tttccgcgta cccggcgttt agttattgcg gatgatgata ttgaagctcg gctgattggt
     1261 tcattgtcgc catcaccgct ggacggtgta ttaagtaaag cgtcaacgct ggagattttt
     1321 catcaggaac tctttttgtc attaaatggt gtacgtcagg cgaccgaccg actgaacaat
     1381 cagtggtaca ttaaccaaag ccggacgcta agcccgacgg agagagaaat attgcgcttt
     1441 atgtcgcgtg gctactcaat gacacaaatt gccgagcagc ttaaacgtaa tatcaaaacg
     1501 atccgtgcac ataaatttaa tgtgatgtcg aaactgggcg tcagttctga tgcagggttg
     1561 ttggaggccg cagatattct gttatgtatg cggcattgcg aaacaagtaa tgtgttgcat
     1621 ccctattaat ccgcatgatg ccgggtttac ttccccggca gtgctttcat ttcagcgtac
     1681 aatcgccaca ttgctgcaca tccggtaagc gataacgctg gcagcaagtg cggcgcacca
     1741 gcaggccatc gcgcagtacc acggtacgcc agagtggatt atcttcaccg ttcgtgagcg
     1801 ttttctcaaa aaagagggca tggcgcagcg attcaaccgt cgcctcgccg agcagttgct
     1861 tcatctcagt aagataccag ttgatcaaat aaccggtatt actccagata agtttgccgt
     1921 tgatctctcc ggtcgcttct agtgcctgca caaccggaac cagcgcctgg ctgattaacc
     1981 tttccattcg ctgctgcggc gaatgtggtg ttgcgttttt atcttcacac acatcgaccc
     2041 agaaacaggc ggcacggccg gtttcgtgaa actcagcatg gaaatgttcc ggcgacacat
     2101 ctaatgcctt ttcctgcgtc agtagcgcca gcattaatgg tggcaccatc aggccgatat
     2161 accattgtgc ccatagtgag atcagcggtt tgttctcgcg gatcatcgtt ggttggttgc
     2221 gatagatatg atcggaatag accgcgagta gagaacttag cgcattcggt gatgaccatt
     2281 gcgccagcgt catggcgtta agcggagcgg gttcatccag gcggataaac tcgagcaaat
     2341 gttcacgatg tttggcgatc gtcgcacgta cggcttgtgc aagcccagca tcctgcggct
     2401 ggagatgcgt tcgccagatg atatcttcat agagcggtgc ggaacgatag gccataatcg
     2461 ggatagtaat ctaaatgata atgattgcta atcctagcga taggtttacc cgatagcaag
     2521 ggatttcttt ggcttgcaaa tgataaaaat tatcatatga tattggttat cattatcaat
     2581 gaaagagatg aaaccatgtt gcaacgtatg ctgggcagtg gctggggagt gttgctgcca
     2641 ggattgctga ttgcagggct gatgtatgcg gatttatcgc cggatcagtg gcggattgtc
     2701 attctgatgg gattagtatt gacgccggtg atgctgtatc acaaacagtt acggcattac
     2761 gttttgctac catcgtgcct ggcgcttatt gctggcatca tgctgatgat aatgaatttg
     2821 aatcagggat gaaaaatcaa ggaagaaaca agaaaggaag taaagata
//
LOCUS       DAZFXT010000260         2806 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.262, whole genome shotgun sequence.
ACCESSION   DAZFXT010000260 DAZFXT010000000
VERSION     DAZFXT010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.262"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..560
                     /locus_tag="ACHKW0_005012"
     CDS             <1..560
                     /locus_tag="ACHKW0_005012"
                     /EC_number="3.1.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lambda exonuclease family protein"
                     /protein_id="HFO7477659.1"
                     /translation="AKPRSGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYE
                     NDARALFEFTSGVNVTESPIIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFR
                     LGGFEAIKSAYMAQVQYSMWVTRKDAWYFANYDPRMKREGLHYVVVERDEKYMAGFDE
                     MVPEFIEKMDEALAEIGFVFGEQWR"
     gene            557..739
                     /locus_tag="ACHKW0_005013"
     CDS             557..739
                     /locus_tag="ACHKW0_005013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1317 domain-containing protein"
                     /protein_id="HFO7477660.1"
                     /translation="MKHPHDNIRVGAITFVYSVTKRGWVFPDLSVIQNPLKAQRLAEE
                     INNKRGAVCTKHLLLS"
     gene            712..903
                     /locus_tag="ACHKW0_005014"
     CDS             712..903
                     /locus_tag="ACHKW0_005014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1382 family protein"
                     /protein_id="HFO7477661.1"
                     /translation="MHKASPVELRTSIEMAHSLAQIGVRFVPIPVETDEEFHTLATSL
                     SQKLEMMVAKAEADERDQV"
     gene            980..1195
                     /locus_tag="ACHKW0_005015"
     CDS             980..1195
                     /locus_tag="ACHKW0_005015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HFO7477662.1"
                     /translation="MTPQQENALRSIARQANSEIKKARQQFPDKNVDDICRSVLKKHR
                     ETVTLMGFTPTHLSLAIGMLNGVFKER"
     gene            1294..1515
                     /locus_tag="ACHKW0_005016"
     CDS             1294..1515
                     /locus_tag="ACHKW0_005016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HFO7477663.1"
                     /translation="MADIIDSASEIEELQRNTAIKMRRLNHQAISATHCCECGDPIDE
                     RRRLAVQGCRTCASCQEDLELISKQRGSK"
     gene            complement(1726..2328)
                     /locus_tag="ACHKW0_005017"
     CDS             complement(1726..2328)
                     /locus_tag="ACHKW0_005017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477664.1"
                     /translation="MSYFLRKKWMVNLSGAGKILWTLNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGMCIFAGFFLIIPLTTLTYFLPGETSSYVAPYRYTSGSSKSCSGAEVDDPDLHEN
                     IRICYPYGNYEYDNIIYVEKKINTLGAVVTYAQTARDDTE"
     gene            2571..2738
                     /locus_tag="ACHKW0_005018"
     CDS             2571..2738
                     /locus_tag="ACHKW0_005018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477665.1"
                     /translation="MHFRVTGEWNGEPFNRVIEAENINDCYDHWMIWAQIAHADITNI
                     RIEELKEHQAA"
BASE COUNT          865 a          611 c          642 g          688 t
ORIGIN      
        1 tagcaaaacc ccgctccgga aaaaagtggc ctgacatgaa aatgtcctac ttccacaccc
       61 tgcttgctga ggtttgcacc ggtgtggctc cggaagttaa cgctaaagcg ctggcctggg
      121 gaaaacagta cgagaacgac gccagagccc tgtttgagtt cacttccggc gtgaatgtta
      181 ctgaatcacc gatcatctat cgcgacgaaa gtatgcgtac cgcctgctct ccagatggtt
      241 tatgcagtga cggcaacggc cttgaactga aatgcccgtt tacctcccgg gatttcatga
      301 agttccggct cggtggtttc gaggccataa agtcagctta catggcccag gtgcagtaca
      361 gcatgtgggt gacgcgaaaa gatgcctggt actttgccaa ctatgacccg cgtatgaagc
      421 gtgaaggact gcattatgtc gtggttgagc gggatgaaaa gtacatggcg ggttttgacg
      481 agatggtgcc ggagttcatc gaaaaaatgg acgaggcact ggctgaaatt ggttttgtat
      541 ttggggagca atggcgatga agcatcctca cgataatatc cgggtaggtg cgatcacttt
      601 cgtctactcc gttacaaagc gaggctgggt atttcccgac ctttctgtta tccaaaatcc
      661 actgaaagcc cagcggctgg ctgaggagat aaataataaa cgaggggctg tatgcacaaa
      721 gcatctcctg ttgagttaag aacgagcatt gagatggcac atagccttgc tcaaattgga
      781 gtcaggtttg tgccaatacc agtagaaaca gacgaagaat ttcatacgtt agccacatcc
      841 ctttcacaaa agctggaaat gatggtggcg aaagcagaag cagatgagag agaccaggta
      901 tgacaaccac tgaatgcatt tttctggcag cgggcttcat attctgtgtg cttatgcttg
      961 ccgacatggg gcttgttcag tgacacctca gcaagaaaac gcccttcgca gcattgcccg
     1021 tcaggctaat tctgaaatca aaaaagccag acagcagttt ccggataaaa acgtcgatga
     1081 catttgccgt agcgtactga agaagcaccg cgaaacggta acgctgatgg gattcacacc
     1141 gactcattta agcctggcga tcggcatgtt aaacggcgtc tttaaggaac ggtgagcatg
     1201 aaaagcaaaa tcatcaggga gctacaggct ccttttttat tgttcgcatt caccctcaag
     1261 cgtattaacc aacaattcag ggattaatgg aagatggcag acatcattga ttcagcatca
     1321 gaaattgaag aattacagcg caacacagca ataaaaatgc gccgcctgaa ccaccaggct
     1381 atatctgcca ctcattgttg tgagtgtggc gatcccatag atgagcgaag acgcctggcc
     1441 gttcagggtt gtcggacttg tgcaagttgc caggaggatc tggaacttat cagtaaacag
     1501 agaggttcga agtgagcgaa attaactaga agccaaagat aaaatcatcg ctgagcagga
     1561 gaaaatcgct aacggagaaa agacagtaag tcagtatatg aaaaccgcat gatatcatca
     1621 gataaaaatc gatcgtaaag cgaaatatta ataccagaat aaacgagtcg aggtaaatta
     1681 tattacctcg ataaattaac taaaacttgc ccgctatata ctatctcatt cagtatcatc
     1741 acgcgcggtc tgtgcatatg ttactaccgc acctaatgta ttaattttct tttcaacata
     1801 gataatatta tcgtactcat aattgccata cggatagcaa atgcgaatat tctcatgtag
     1861 atcggggtca tccacctcag ctccagaaca actttttgaa ctaccggaag tataccgata
     1921 cggtgcaaca taagacgatg tctctccagg caaaaaataa gttagtgttg taaggggtat
     1981 aatcagaaaa aatccagcaa atatgcacat ccctgcataa accttaaggt atgctgacag
     2041 actcttccag ccgctttgtt ttactatccc cttcttaacc caaaacagag ataacagaaa
     2101 agctattccc atgctaaaca gaatgtaata gtgggatata ctctgattaa gaaacgtgac
     2161 cctgtaaata tctgcccgcc accagaagaa aaggaaaata aagatcaggc ctgaaactgt
     2221 catgcaaatc aaataaggat acgaatcttt tttcatgttt agcgtccata aaatttttcc
     2281 tgccccggac aaatttacca tccatttttt gcgcagaaaa tagctcatta cttactgcac
     2341 aataatacac aaaattgcgt aaattttttg catggatttt agctctttca gccgacgttt
     2401 aaggggtaaa tagcatttcc taaaagcaac tgcaccaacc caacagaatg agctaccgct
     2461 tacgttgaga gcaaaaaagt gtatagcagc aatgaacagc atcctcgcac tgacgaggat
     2521 ttcttttatc tgaactcgct acggcgggtt ttgttttatg gagatgataa atgcacttcc
     2581 gagtcacagg tgaatggaat ggagaaccat tcaacagagt tatcgaagca gagaacatca
     2641 atgactgcta tgaccactgg atgatatggg cgcagatagc acatgcagac ataaccaata
     2701 ttcgaattga agaactgaaa gaacaccaag ccgcctgatg gcggcttttt cttgcgtgta
     2761 attgcggaga ctttgcgatg tacttgacac ttcaggagtg gaacgc
//
LOCUS       DAZFXT010000261         2785 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.263, whole genome shotgun sequence.
ACCESSION   DAZFXT010000261 DAZFXT010000000
VERSION     DAZFXT010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.263"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..810
                     /locus_tag="ACHKW0_005019"
     CDS             <1..810
                     /locus_tag="ACHKW0_005019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein"
                     /protein_id="HFO7477666.1"
                     /translation="DFLNGDPDQPIIMGRTYHQENRTPGSLPGTKTQMTIRSKTYKGS
                     GFNELKFDDATGKEQVYIHAQKNMNTEVLNNRTTDVINNHAETIGNNQMIAVTNNQIQ
                     TVGVNQIETVGSNQIINVGSVQVETIGLVRALTVGVAYQTTVGGIMNTSVALMQSSQI
                     GLHKSLRVGLGYDVKVGNNVTFTVGKTKKDDTGQTAIYSAGEHLELCCGKARLVLTKD
                     GQIFLNGTKIHLQGKEQVNGDSLLINWNCAASKSPPKTPDEKQDTPDMREY"
     gene            877..>2785
                     /gene="rhsD"
                     /locus_tag="ACHKW0_005020"
     CDS             877..>2785
                     /gene="rhsD"
                     /locus_tag="ACHKW0_005020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HFO7477667.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTRTPAPVGVFGPGWKAPSDI
                     RLQLRDDALVLNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSERVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARTSSLSSSDSSRPLSAS
                     PFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYDRSN
                     TQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYQYEQDRIT
                     VTDSLNRREVLHTEGGAGLKRVVKKELADGSVTHSGYDAAGRLTAQTDAAGRRTEYGL
                     NVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSRSGE
                     TVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQMTA
                     VHREEGISLYRHYDNRGRLTSVKDAQGRETQYEYNAAGDLTAVITPDGNRSETQYDAW
                     GKAVSTTQGGLT"
BASE COUNT          628 a          767 c          920 g          470 t
ORIGIN      
        1 gacttcctca acggcgatcc ggaccagccg attattatgg ggcgtaccta ccaccaggaa
       61 aaccgcacac ccggcagcct gccggggacg aagacgcaga tgaccattcg ttcgaaaacc
      121 tataagggca gcgggtttaa tgaactgaag tttgacgatg cgacagggaa agaacaggtc
      181 tacatccacg cgcagaagaa catgaacacc gaggtgctga ataaccgcac cacggatgtg
      241 ataaacaacc atgctgagac cattggcaac aatcagatga ttgcggttac caacaatcag
      301 atacagacgg tgggcgttaa ccagatagag acggtgggca gtaaccagat cattaacgtg
      361 ggttctgttc aggtggaaac gattggactt gttcgtgcgc tgaccgtggg cgtggcgtac
      421 cagacgacgg taggtggcat tatgaacact tcggtggctc tgatgcagtc ctcgcagatc
      481 ggtttgcata aatcgttgag ggtcgggctg ggttatgacg tcaaagtcgg aaataacgtt
      541 accttcaccg ttggtaaaac gaaaaaggat gataccgggc agaccgcgat ttactccgcc
      601 ggtgagcatc tggagctctg ctgtggtaag gcaaggctgg tgctgacgaa ggacggacaa
      661 atttttctca acggcacaaa aattcatttg cagggtaagg agcaggttaa tggtgactca
      721 ctgttgatta actggaactg tgcagcctcg aaatccccac cgaagacccc tgatgaaaag
      781 caggatacgc cggatatgag agagtactga tcccgtacct gttcggtatt cagtaacaaa
      841 caagcattaa aaataaacag aagaaggaat tgaatcatga gcggaaaacc agcggcgcgt
      901 cagggagata tgactcagta tggcggtcct attgtccagg gttcggcagg tgtaagaatt
      961 ggcgcgccca ccggcgtggc gtgctcggtg tgtccgggcg ggatgacttc gggcaacccg
     1021 gtaaatccgc tgctgggggc gaaggtgctg cccggcgaga cggaccttgc gctgcccggc
     1081 ccgctgccgt tcattctctc ccgcacctac agcagctacc ggaccaggac acccgcgccg
     1141 gtgggagtgt ttggccccgg ctggaaagcg ccttctgata tccgcttaca gctacgcgat
     1201 gatgcactgg tactcaacga caacggcggg cggagcattc actttgagcc gctgctgccg
     1261 ggggaggcgg tgtacagccg cagcgagtca atgtggctgg tgcgcggtgg taaggcagcg
     1321 cagccggacg gccacacgct ggcgcggctg tggggggcgc tgccgccgga tatccggtta
     1381 agcccgcatc tttacctggc gaccaacagc gcacaggggc cgtggtggat actggggtgg
     1441 tcagagcggg tgccgggtgc tgaggacgta ctgccagcgc cgctgccgcc gtaccgggtg
     1501 cttaccgggc tggcagaccg tttcgggcgg acgctgacgt accggcgtga ggccgcaggc
     1561 gacctggccg gggaaatcac cggcgtgacg gacggtgccg ggcgtgagtt ccgtctggta
     1621 ctgaccacac aggcacagcg ggcggaagag gcccgcacct cttcgctatc ttcttctgac
     1681 agttcccgcc ctctctcagc ctcaccgttc cccgacacac tgcccggtac cgaatacggc
     1741 cccgacaggg gcatccgtct ttcggcggtg tggctgatgc acgacccggc atacccggaa
     1801 agcctgcccg gtgcgccact ggcgcggtac acgtatacgg aagccggtga actgctggcg
     1861 gtatatgacc gcagcaatac gcaggtgcgc gctttcacgt atgacgcgca gcacccgggc
     1921 cggatggtgg cgcaccgtta cgcgggaagg ccggagatgc gctaccgcta cgacgatacc
     1981 gggcgggtgg tggagcagct gaacccggca ggcctgagtt accgctacca gtatgagcag
     2041 gaccgcatca ccgtcacgga cagcctgaac cggcgtgagg tgctgcatac agaaggcggg
     2101 gccgggctga agcgggtggt gaaaaaagaa ctggcggacg gcagcgtcac gcacagcggg
     2161 tatgacgcgg caggaaggct cacggcgcag acggacgcgg cgggacggcg gacagagtac
     2221 ggtctgaatg tggtgtccgg cgatatcacg gacatcacca caccggacgg gcgggagacg
     2281 aaattttact ataacgacgg gaaccagctg acggcggtgg tgtccccgga cgggctggag
     2341 agccgccggg aatatgatga accgggcagg ctggtatcgg agacatcgcg cagcggggag
     2401 acagtacgct accgctacga tgacgcgcac agtgagttac cggcgacgac aacggatgcg
     2461 acgggcagca cccggcagat gacgtggagc cgctacgggc agttgctggc gttcactgac
     2521 tgctcgggct accagacccg ctatgaatac gaccgcttcg gccagatgac ggcggttcac
     2581 cgcgaggaag gtatcagcct ttaccgccac tatgacaacc gtggccggtt aacctcggtg
     2641 aaagacgcac agggccgtga aacgcagtat gagtacaacg ccgcaggcga cctgactgcc
     2701 gttatcaccc cggacggcaa ccggagcgag acacagtacg atgcgtgggg aaaggcggtc
     2761 agcaccacgc agggcgggct gacgc
//
LOCUS       DAZFXT010000262         2772 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.264, whole genome shotgun sequence.
ACCESSION   DAZFXT010000262 DAZFXT010000000
VERSION     DAZFXT010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.264"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1154
                     /locus_tag="ACHKW0_005021"
     CDS             join(<1..235,235..1154)
                     /locus_tag="ACHKW0_005021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612262.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HFO7477668.1"
                     /translation="FTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLESWVRQLRRE
                     RQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERFTIAARLSD
                     SHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSAGARTLAEM
                     LTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQFAVPEPDRV
                     WCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAYETRGQPRD
                     VVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKTEWVPTNGY
                     AGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVANIT"
     gene            1164..1715
                     /locus_tag="ACHKW0_005022"
                     /pseudo
     CDS             1164..1715
                     /locus_tag="ACHKW0_005022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
     gene            1715..1960
                     /locus_tag="ACHKW0_005023"
     CDS             1715..1960
                     /locus_tag="ACHKW0_005023"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477669.1"
                     /translation="MQHVSEGVIQVPELPDAPEALPGCRSISDDDLCAWCTRLLYRPG
                     ELSLCRLSTGNGIWPSVCDRNGYAYGCPEFQQNIIHP"
     gene            2054..2527
                     /locus_tag="ACHKW0_005024"
     CDS             2054..2527
                     /locus_tag="ACHKW0_005024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7477670.1"
                     /translation="MKTLSQNTTSSACAPETGLQQLVATIVPDEQRISFWPQHFGLIP
                     QWVTLEPRIFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDENWVLFNAMNGN
                     RAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
     gene            2543..>2772
                     /locus_tag="ACHKW0_005025"
     CDS             2543..>2772
                     /locus_tag="ACHKW0_005025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO7477671.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETL"
BASE COUNT          663 a          744 c          765 g          600 t
ORIGIN      
        1 ttcacccctg agttcaggct ggaatgtgca cagctgattg ttgataaggg ctactcatat
       61 cgacaggcca gtgaagcgat gaatgtcggt tcaaccacgc ttgagagctg ggtacgccag
      121 ctcaggcgag agcgccaggg tattacgccc tctgccacac ccattactcc agaccagcaa
      181 cgtatccgcg agctggaaaa gcaagttcgc cgtctggagg aacaaaatac gatattaaaa
      241 aaggctaccg cgctcttaat gtccgactcg ctgaacggtt cacgatagcc gccagactaa
      301 gtgacagcca cacggttgtc agcctgtgtt ctgctctgga aatacaccgc agcagttacc
      361 ggtactggcg aaaacgacgc gatacggtta atccggcacg agtcaggttg tgcagcgaaa
      421 tacgccgggc gtggaaccaa agtcggggct ctgcgggcgc gcgcactctg gctgaaatgc
      481 tgactcaaaa cggcgtcccg atgagccgtt accgtgccgg acgtctgatg aaatatctga
      541 acctgagcag ttgtcagccc ggaaaacatc agtataaaaa tgcccgtcag gagcatacca
      601 gcctgccgaa tctgcttgag cgtcagttcg cagtaccgga gccagaccgg gtatggtgtg
      661 gagatattac gtatctctgg gcaggaaatc gctggtgcta tctggcggtt gttatggatc
      721 tttttgcccg cagggttatc ggctggagtc tgtcagcgca tgccgatacc gcactgataa
      781 gcagtgccct gcggatggcc tatgagacgc gtggccaacc ccgggatgtc gtgttccata
      841 gcgaccaggg aagccagtat acaggcctta aatatcaaca acttctctgg cgttgcagaa
      901 taaatcaaag cgtcagtcgg cggggaaact gctgggataa cagccccatg gaacgcttct
      961 tccgcagtct gaaaacagaa tgggtgccaa cgaatggtta cgcaggcaag gacgaggccc
     1021 ggcagcaaat taatgattac atattgaact actacaacag cgtcagacct caccattata
     1081 acggtgggct gacgccggaa gagtcagaga acagatacca tttttactgt aaaaccgtgg
     1141 ccaatattac ttgaccacta cacggagaga taaacggaga acatgtccct gaaattattc
     1201 tgctcaactc tcatgacggt acctccagct accagatgct gccgggttac ttcaggttcg
     1261 tctgccagaa cggatgcgtc tgtggtcagt ctctggggga agtgcgtgtt ccgcaccggg
     1321 gaaatgtggt ggacagagtc attgaagggg cttacgaggt ggtgggcgtg tttgaccgga
     1381 tagaggagaa gcgtgatgcc atgcagtcgc tgctcttacc gccaccggca cgccaggcgc
     1441 tggcacaggc ggcactgact taccgttatg gtgatgaaca tcagcccgtc accactgccg
     1501 acattctgac accacgacgc cgggaggatt acggtcagga cctgtggagt gcttatcaga
     1561 ccattcagga gaatatgctg aaaggcggaa tttccggtcg cagtgccaga ggaaaacgta
     1621 tccacacccg tgccattcac agcatcgaca ccgatattaa gctcaaccgc gcattgtggg
     1681 taatggcaga aacgctgctg gagagcctgc gctgatgcag catgtctctg aaggcgtgat
     1741 tcaggtacca gaattacccg acgccccgga ggcgctgccg gggtgtcgtt ccatttctga
     1801 tgatgacctg tgcgcctggt gtacccgtct gctttaccgt cccggagaac tcagtctgtg
     1861 cagactgagt acaggcaatg gcatctggcc atccgtctgt gaccggaacg gttacgcgta
     1921 cggctgtccg gaatttcagc agaatatcat tcatccctga tattaccggg tctcacggtc
     1981 agtgatgaac gtgatgttgc cagctcccga cctttactcc ctctcattta ttcacattac
     2041 aaggatttca tacatgaaaa cattgtctca gaacaccacc tcttcggcct gtgcaccgga
     2101 gactggcctg caacaactgg ttgccaccat agtccctgat gaacagcgca tcagcttctg
     2161 gccgcagcac tttggcctca ttccacagtg ggtgaccctg gagccccgta tcttcggctg
     2221 gatggaccgt ctttgcgaag actactgcgg tggtatctgg aatctgtaca cactgaacaa
     2281 cggtggcgca tttatggcgc ccgaaccgga tgacgatgat gacgaaaact gggtactgtt
     2341 caatgccatg aacggtaacc gcgctgaaat gagcccggaa gccgccggca ttgccgcctg
     2401 tctgatgacg tacagtcatc atgcctgtcg tacggagtgt tatgccatga cggtccatta
     2461 ttaccggttg cgggattacg ccctgcagca tccggaatgc agcgccatta tgcgcatcat
     2521 cgactgaatg gagagaagca caatgcaaca actttccttt ctgcccggag agatgacgcc
     2581 cggcgagcgc agtctcattc tgcgggccct gaaaaccctg gaccgccatc ttcatgaacc
     2641 cggcgtggcc ttcacctcca cgcatgcggc acgggaatgg ctgattctga acatggcggg
     2701 actggagcgt gaagaattcc gggtgctgta tctgaataac cagaatcagc tgattgccgg
     2761 tgaaaccctc tt
//
LOCUS       DAZFXT010000263         2752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.265, whole genome shotgun sequence.
ACCESSION   DAZFXT010000263 DAZFXT010000000
VERSION     DAZFXT010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.265"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..1249)
                     /gene="aspC"
                     /locus_tag="ACHKW0_005026"
     CDS             complement(59..1249)
                     /gene="aspC"
                     /locus_tag="ACHKW0_005026"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HFO7477672.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(1434..2522)
                     /gene="ompF"
                     /locus_tag="ACHKW0_005027"
     CDS             complement(1434..2522)
                     /gene="ompF"
                     /locus_tag="ACHKW0_005027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HFO7477673.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT          702 a          668 c          653 g          729 t
ORIGIN      
        1 tgttgcgttt gtcatcagtc tcagcccgct tttcagcggg cttcattgtt tttaatgctt
       61 acagcactgc cacaatcgct tcgcacagcg gagccatgtt atctggtgtc atcccggcca
      121 catttacgcg accagaagca accgcatata cgccaaactc ttcgcgcaga cgcagcactt
      181 gttcttttgt caggccactg aaggagaaca tgccgttctg tttgatgata aagctgaagt
      241 cgcggtttgc gcctttttcc tgcagcgtat tgacgaacaa ctgacgcata cgctgaatac
      301 gctggcgcat atcagtcagc tcttgttccc aaatcgcacg taacgcatcg ttgctcagga
      361 tggtggcaac aacagaagcg ccgtgtgctg gtgggttaga gtagttagcg cgaatcgccg
      421 ctttcatttg gctgaatgcg cgatcaacgg tttcactgtc ggcagcaacc agagtacaag
      481 cgccaacacg ctcgttgtac aggccaaagt ttttagagta ggaactggca acaatcagct
      541 ctttatgcat agccgcgaaa gcgcgcagtc cttcagcatc ttcttccaga ccacgggcaa
      601 aaccctggta agcgaagtca aacagcggta accagccttt ctcaacggag agttgtgcca
      661 gtgtctgcca ttgttccagc gtagggtcga taccggttgg gttatggcag cagccatgga
      721 acagcactac gtcgccagcc tgagcttcat tcaggctgtt aatcagtgca tcgaagtcaa
      781 gagtgtgatt ttccgcatca taataagcgt attcacgaac ttccagacct gcagagttaa
      841 agacgctctt atggttcggc cagcttgggt tgctcaccca cactcgctta acgctggtat
      901 tttttgccag gaaatcggca gctacgcgta gtgcgccagt gccccccgga gtctgtgccg
      961 tgcgagcacg tttgtcattg atcagggcgc tacctttacc aaacagcagt tcctgagtgc
     1021 agcgaccaaa ttcagggatg ccgtcaatgc cgaggtaatt tttggtggtt tcattttcga
     1081 gcagatactg ttcagccttt ttcacgctgg tcagtaccgg ggttttgccc gtctcatctt
     1141 tatagacacc aatcccgagg ttaattttgc cgggacgttc atcggcacga aacagatcgg
     1201 ccaggcccag aatcgggtcg gcaggagcgg cggtaatgtt ctcaaacatg acgaggttcc
     1261 attatggtta cagaagggaa gtccgctatc agggtaacgg gagatttaca aaattccaac
     1321 tattactgat gaaaacgcag gctgtttttg caagacgtga gattgctctg gaaggtataa
     1381 aaaaaacagg accaaagtcc tgttttttcg gcatttaaca aagaggtgtg ctattagaac
     1441 tggtaaacga tacccacagc aacggtgtcg tctgaaccta cgcccagttt gttgtcagaa
     1501 tcgatctggt tgatgatgta gtcaacatag gtggacatgt ttttgttgaa gtagtaggtt
     1561 gcgcccactt caaagtagtt caccagttca acatcaccga taccttctac gtctttcgct
     1621 ttagatttgg tgtaagcgat ggacggacgc agaccgaaat cgaactggta ttgcgcaact
     1681 aacagaacgt cttgcgtttt gttggcgaag ccgctggtgt ttgtaaattt attagtgatc
     1741 ggcgtagcgt tacgggtttc accgtagttc gctgccaggt agatgttgtt cgcgtcgtac
     1801 ttcagaccag tagcccactg ttcagctttt ttaccgttgc caagaggttg agcttcttgc
     1861 aggttggtac ggtcagctgc accataagca ccaacgatac caaagccttc gtattcgtag
     1921 ctgatagaac cgccaacacc gtcgccgtta gaacggcgtg cagtgtcacg ctcgttttta
     1981 cccaggtact gaacagcgaa gttcaggcca tcaaccagac caaagaagtt ggagttacga
     2041 taggtagcaa cgccgccaac acgaccaacg aagaagtcat cgctgtatgc agtatcacca
     2101 ccaaattctg gcagcatatc ggtgtaaccc agtgcatcat aaaccacacc gtagttacgg
     2161 ccgtaatcga aagaaccaac gtcagcgtat ttaagacccg cgaatgccag acgcgttttg
     2221 ttaccagttt gagcgtcagc gccttcagag ttgttaccct ggaagttata ttcccactga
     2281 ccataaccgg tcagatcgga attgatttga gtttcccctt taaaaccaag acgggcatag
     2341 gtcatgtcgc cattgccacc gtaactgttt tcaccgttac ccttggaaaa ataatgcaga
     2401 ccaacagctt taccgtacag atctactttg ttgccatctt tgttatagat ttctgcagcg
     2461 tttgcagtac ctgctactaa cagagcaggg acgatcactg ccagaatatt gcgcttcatc
     2521 attatttatt accctcatgg ttttttttat gacacctgcc actgccgtca ataagttctg
     2581 tcaataaaaa tttacggaac tattgatgag agtttggtgt ctttatgtgt ctgcaggcat
     2641 ctttccattc aaactaacgt ttcgctaccg tgaaagtgct acaaagataa agatttagtt
     2701 tcaaaaagaa aaaatatgta accaaaagta aaaattaagg aactttgtga ac
//
LOCUS       DAZFXT010000264         2752 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.266, whole genome shotgun sequence.
ACCESSION   DAZFXT010000264 DAZFXT010000000
VERSION     DAZFXT010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.266"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..266
                     /locus_tag="ACHKW0_005028"
     CDS             <1..266
                     /locus_tag="ACHKW0_005028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO7477674.1"
                     /translation="RDCFVEMLQDKNEEVRIEAIIGLSYRKDKRVLSVLCDELKKNTV
                     YDDIIEAAGELGDKTLLPVLDTMLYKFDDNEIITSAIDKLKRS"
     gene            294..1252
                     /locus_tag="ACHKW0_005029"
                     /pseudo
     CDS             294..1252
                     /locus_tag="ACHKW0_005029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
     gene            1264..1725
                     /gene="yibG"
                     /locus_tag="ACHKW0_005030"
     CDS             1264..1725
                     /gene="yibG"
                     /locus_tag="ACHKW0_005030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HFO7477675.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTKNNIDKQLRFLERSASQGNVTAQFNYGVFLSD
                     TNPTLSEYYNLNRAIYWMEFAVNNGNIDAKSKLQELKKLKRMDRRKNKENP"
     gene            2360..>2752
                     /locus_tag="ACHKW0_005031"
     CDS             2360..>2752
                     /locus_tag="ACHKW0_005031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016158939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Imm57 family immunity protein"
                     /protein_id="HFO7477676.1"
                     /translation="MNNCIFFSISASAVTARQFQRELDLADKAILWALISASTKEGRK
                     ACSLSYFACKAAEAELGLAYMAANDNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGK
                     IIRPYLKNLNPLQLAADCIETVNKIKDKN"
BASE COUNT          876 a          488 c          626 g          762 t
ORIGIN      
        1 ttagggattg ttttgtggag atgcttcagg ataaaaatga ggaagtccgt attgaagcaa
       61 taatcggact ttcctacaga aaagataaaa gggttttatc tgttttatgc gatgagttaa
      121 aaaaaaatac tgtttatgat gatatcattg aagctgcggg tgaattaggt gataaaacgc
      181 tacttcctgt tttagatact atgttgtaca agtttgatga caatgaaatt ataacttccg
      241 ctattgataa gctgaagcgt tcatagcgtg agttgcctat gcacagtggg ggattcccgc
      301 cggcacggtg cagatgctcg accggctgga aagtgaaatc ctggctgacc gggtgagtga
      361 ggaaagccgc cgctggctgg catcgtgcgg cctgaccgtg gagcagatga aaaaccagat
      421 ggacccggtg tacacgccgg cgcgaaaaat ccacctgtac cactgcgacc atcgcggcct
      481 gccgctggcg ctcatcagca cggaaggggc aacagcgtgg tgcgcagaat atgatgaatg
      541 gggcaacctg ctgagtgatg agaacccgca tcatctgcag cagctcattc gcctgccggg
      601 tcagcagtat gatgaggagt ccggcctgta ttacaaccgc caccgctatt atgacccgct
      661 gcaggggcgg tatatcactc aggatccgat tgggctgaag gggggatgga atttttatca
      721 gtatccgttg aatccggtca taaatgtaga tccgcaaggt ttggttgata taaatttata
      781 ccccgaaagt gatcttatcc attctgtagc tgatgagatt aatatcccag gcgttttcac
      841 aatcgggggg catggtaccc ccacatctat tgaatccgca acgcgcagta tcatgacagc
      901 taaagatcta gcatatctaa ttaaatttga tgggaattat aaagatggga tgacagtttg
      961 gttattttct tgtaatacag gtaaaggaca aaattcattt gctagccaat tagctaaaga
     1021 gttacataca aatgtaatag gacctgacac gctatggacg tggtgggggc gaggaactaa
     1081 tggtaagtta aaaatggata cagtgctaac agcaccaacg aaccttaatt caaataagga
     1141 tctaatggct ataacaacaa aagaccttgg taattggata acatatgggc catctgggca
     1201 ccccatttct aatatgcaag gtacgccaga aaaacccagt gatataagat aggttgtaga
     1261 tgtatgaaag catgcttgtt actatttttt tatttttctc ttattggtca attgcatggt
     1321 gctgatgtga aaataaaaga aaacgaaagt gtgatgggat ctacagcaat gacctatgat
     1381 ttaagtgaag aaaagctgat gaaactgaag tataaatcac aacatggtga ttcagaggca
     1441 tcatttcgac tatatcaata ttactgcttt actaaaaata atattgataa acaattgcga
     1501 ttcttggaaa gatcagcatc tcaggggaat gttacagcgc agtttaatta tggggtcttt
     1561 ttatcagata caaatccaac attatcagaa tattataatt tgaatagagc tatttattgg
     1621 atggaattcg ctgtaaataa cggtaatatt gatgcaaaga gcaaacttca agagctcaaa
     1681 aagctaaagc gaatggatag aaggaagaat aaggagaatc cgtaaaagca atagttcatc
     1741 agactgccgc agcagtagta tgatgatgaa tcagggctgt actataacta ttatcggtaa
     1801 cattaaccgc agcaggggcg gtataacact caggctccga ttgggctgga aggttggtgg
     1861 aatttttatc agtatccgtt gaacccgatt tctggaatcg atccccttgg gattagccac
     1921 ctgtttatac agtataactt taggtatgct ttcgtgtgtt tcagatatac catgtgatga
     1981 taatttttat gatgtgctta gtattccagt cgcttctggt aataatatgc aatgtaaaaa
     2041 taatccccgt tgtacacatt tataaataga tggccaatac ctcaaggcct atggagttag
     2101 aatgttaatg acccgggagc aagcaataga aaacctgacg gcatacgatt agtaccgtca
     2161 gttaatacag gaacttataa tcgcaatgga ttttcaatac atagttgttt aaacgctttt
     2221 ggtccgtctt taggacctcg attctgttcc gagggttgta taactggttt atctaatgac
     2281 atgcaaaaat taaatgagtt aatattttca gagccagata gcacattaac agttacggac
     2341 taaaaaatat ggcaaggtta ttaacaattg tatttttttc tctatttctg cgagtgcagt
     2401 tactgcaaga caatttcaga gagagttaga tttggcagac aaagcaatat tatgggcact
     2461 aatatcagcc agtactaaag agggacgaaa agcgtgttca ctaagctatt ttgcctgtaa
     2521 ggccgcagag gctgaactcg gactggcata tatggctgcg aatgataaca aggaatttct
     2581 cacatcctta tctaatataa tgaggtacaa aatagatgct ggactttccg aatcctatac
     2641 atgctattta ttaagtaagg gaaagatcat aagaccatat ctgaaaaatc taaatccact
     2701 ccaacttgcc gctgattgca ttgaaacagt aaataaaata aaagataaga at
//
LOCUS       DAZFXT010000265         2750 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.267, whole genome shotgun sequence.
ACCESSION   DAZFXT010000265 DAZFXT010000000
VERSION     DAZFXT010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2750)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2750)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2750
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.267"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..122)
                     /locus_tag="ACHKW0_005032"
     CDS             complement(<1..122)
                     /locus_tag="ACHKW0_005032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477677.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVT"
     gene            complement(115..375)
                     /locus_tag="ACHKW0_005033"
     CDS             complement(115..375)
                     /locus_tag="ACHKW0_005033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7477678.1"
                     /translation="MQSPLRNVRKAHGFTLQHVAAGVQVNPATLSRIERLEQIPSIEL
                     AERLANFFKGEVSEMQILYPARFQSSQNQNGFKPQEQEVNRG"
     gene            473..1165
                     /locus_tag="ACHKW0_005034"
     CDS             473..1165
                     /locus_tag="ACHKW0_005034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001358750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HFO7477679.1"
                     /translation="MNIGNRVRQLRQAKNMKIADLAEAIGVDAANISRLETGKQKQFT
                     EQTLSNIAKSLGVDIADLFTSAHKSNTVYKNSNNEDVAQVKDVFRIEMLDISASAGNG
                     LIQGGDVIDVIHAIEYRTDNAVSMFGGRPANHIKVINVRGDSMCPTIEPGDLIFVDVS
                     INQFDGDGIYVFGFDDKIYVKRLQMIPDKLLVISDNQIYREWGITSENEHRFMVFGKV
                     LISQSQTLKRHN"
     gene            complement(1485..2000)
                     /locus_tag="ACHKW0_005035"
     CDS             complement(1485..2000)
                     /locus_tag="ACHKW0_005035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477680.1"
                     /translation="MIAELTAAMTAIRETAQIAKLMNEAKTQAEVNAAIGELNSKLAS
                     IQRECVSLVELVGSYQEINASLKAKIAEFENFEAQTEGYILNQLESGTFVYSKEVIVN
                     GGSITMHLCPKCFGQKIVSILQPFPVSEDELFHKSRCLHCENKFLMNKNPDYVSPPSI
                     EELSRKLNGNL"
     gene            2145..2456
                     /locus_tag="ACHKW0_005036"
                     /pseudo
     CDS             2145..2456
                     /locus_tag="ACHKW0_005036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2471..>2750
                     /gene="yfdP"
                     /locus_tag="ACHKW0_005037"
     CDS             2471..>2750
                     /gene="yfdP"
                     /locus_tag="ACHKW0_005037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO7477681.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGR"
BASE COUNT          769 a          637 c          613 g          731 t
ORIGIN      
        1 tctgttacat ccagccagtc agcagcttca gcgtaccccc ccggcaacgc tgcgatagtt
       61 tttctgacag ctttcacgta ccactcaggc tgtttttcta ctttccagtg atgcttaccc
      121 acggttcacc tcctgttcct gtggtttaaa cccattctgg ttttggctag attgaaaacg
      181 tgccggataa agaatctgca tttcgctgac ttcaccctta aaaaaattgg ctaaacgttc
      241 tgcaagctcg atagatggaa tctgctccag cctctcaata cgactcaacg tcgctggatt
      301 gacttgaaca cccgcagcaa catgctgcaa agtgaaacca tgcgccttac gcacatttcg
      361 taatggtgat tgcatacgtc ctccaaatat tgcgcgttat gcatgttatt tcacgcaatt
      421 attttgcgca agttgatttg cttatcacgc aataaagaaa tgtaataaac gcatgaacat
      481 aggaaaccga gtcagacaac ttcgccaagc gaagaacatg aaaatcgccg atctcgctga
      541 agcgatagga gtagatgcgg cgaacatctc gcgcttagaa acgggtaagc aaaaacaatt
      601 taccgaacaa acactgagta atattgccaa gagcttaggt gttgatattg ctgatctctt
      661 tacctctgcc cacaaaagta atactgtata taaaaacagt aataatgagg atgttgcgca
      721 ggtgaaggat gtgttccgta ttgaaatgct ggatatcagt gccagtgcgg gaaatggcct
      781 tatccagggc ggtgatgtca ttgatgtgat tcatgccatc gaatacagaa ctgataatgc
      841 tgtatcaatg ttcggcggac gaccagccaa tcacatcaaa gttatcaacg ttcgtgggga
      901 cagtatgtgt ccaaccattg agccaggaga tctcatcttc gttgatgtca gcatcaatca
      961 gtttgatggt gatggtatat atgtctttgg ttttgatgac aaaatatacg ttaaaagact
     1021 tcaaatgatt cctgacaaac tgctggtgat ttctgataac cagatttacc gtgaatgggg
     1081 aattactagc gaaaacgaac accgattcat ggtctttgga aaggtcttaa tcagtcagtc
     1141 gcaaaccctt aagagacata attaacctca atatcccatc catcggccac cgaaaggtgg
     1201 ctttttatta cctataaatt tgcatatcac gcaaatatca cttgcatatc tcgcaattta
     1261 attttatctt ttgttccaga ccaactacag gattacaaca aaatctggtt gcaacacggt
     1321 gcatgtgtcg taagcagtca gtaaatgtca aaaacgaaca ggcaggacgc ccacgaagta
     1381 gccgcctggg gcatatgaag tccaggatga ttcgttagca acaaaaaagc gccctacagg
     1441 acgcttagct ctttaacaat ctggatatcc acaacagtag taatctacag attgccgtta
     1501 agttttctgg acaactcctc aatggatgga ggcgatacgt aatccggatt tttattcatc
     1561 agaaacttat tttcacagtg gaggcacctg cttttatgaa aaagctcatc ttcgctaacc
     1621 gggaatggtt gaagtatcga tactatcttt tgtccaaaac attttgggca aagatgcatg
     1681 gttatgctgc caccgttcac gattacctcc ttcgagtata caaaagtacc cgactcaagt
     1741 tggttaagga tatagccttc cgtctgagcc tcaaagtttt cgaattctgc aattttagct
     1801 ttgagagaag catttatttc ttgataagag cccaccagtt caacgagaga cacgcattcg
     1861 cgctgaatag acgcaagctt tgagttcagc tcaccaatag ccgcatttac ttcagcttga
     1921 gtttttgcct cgttcattag ttttgcaatc tgggctgttt cacgaatagc cgtcattgct
     1981 gccgttaatt cagcgatcac attgaatact cttattgttg ttggggatat ccagattaac
     2041 cgaatccttg ttgttgggga ataaccaggt ccacctcgcc tgatgtggct aaaagcaggc
     2101 acataacagc taagtatttt caaccagaga gaatccttag cgttgtggtg aatgcggctc
     2161 agcgcacgcg ggttaaggtt gaggctgaca gtcgaccttc tgtggatacc cacccgcctg
     2221 gtgtgcaacc ttcgccaggc accgggaggc acccggcacc acaactttat gctgtgtgta
     2281 gtcctggcgg taccagtttg tacccttgct tccggctggt accgtccttt tttgcaaaac
     2341 agagaagagc atcaccggac gacgggctca taacccaatc catccgggcg gctgccaccg
     2401 caggtgttct tctctgtttt gtggagaaac caaccgacct tgcaaggtcg atatgatgag
     2461 gagcagcaaa atggctagcg aacgcagtac tgatgtgcag gcatttatcg gggagctgga
     2521 cggcggcgta tttgaaacca aaatcggcgc agttctcagt gaggtcgctt ccggtgtgat
     2581 gaacacgaaa accaaaggga aggtctcact caatctggaa atcgaaccgt ttgatgagaa
     2641 ccgtgtgaaa atcaaacaca aactctcata tgttcgccca actaaccgcg ggaaaatttc
     2701 cgaagaagac accaccgaaa cgccgatgta tgtcaatcgc ggtggtcgcc
//
LOCUS       DAZFXT010000266         2695 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.268, whole genome shotgun sequence.
ACCESSION   DAZFXT010000266 DAZFXT010000000
VERSION     DAZFXT010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.268"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            511..654
                     /locus_tag="ACHKW0_005038"
     CDS             511..654
                     /locus_tag="ACHKW0_005038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2627 domain-containing protein"
                     /protein_id="HFO7477682.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            667..876
                     /gene="cspE"
                     /locus_tag="ACHKW0_005039"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             667..876
                     /gene="cspE"
                     /locus_tag="ACHKW0_005039"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HFO7477683.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            1042..1851
                     /gene="rlmA"
                     /locus_tag="ACHKW0_005040"
     CDS             1042..1851
                     /gene="rlmA"
                     /locus_tag="ACHKW0_005040"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HFO7477684.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1848..2414)
                     /gene="mntP"
                     /locus_tag="ACHKW0_005041"
     CDS             complement(1848..2414)
                     /gene="mntP"
                     /locus_tag="ACHKW0_005041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HFO7477685.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          712 a          622 c          637 g          724 t
ORIGIN      
        1 ttttcctcat taatttatta ggatgtttac atcggatttg tgattaagcg tggtattatt
       61 tattagcgaa acgtttctct cttgattttt ttgctcattc atcaattttt cttattttaa
      121 attttcaatc cctttgggga ttgacttctc tttagggtaa ttgatagccg ttaactgact
      181 gttttatgag aaaaagtgat ataacttttt attcattgca tagcaaaaaa tgtgatattg
      241 cacgcactat gtaataactt ctcccactgg cctggaacaa ctgaacttat tgaactatgt
      301 tagaaaatac gccagtttaa gtatctgcct gaactggcaa ggttaagcac aatgatatat
      361 cggcgcgtat tccgttgcat aagtgtgcaa aaaaagtgga agacgtatcg agatttgtgc
      421 gtctgatcga gacatgttta aaaatggctt gccataatta acgttgtatg tgataacaga
      481 tttcgggtta aacgaggtac agttctgttt atgtgtggca ttttcagtaa agaagtcctg
      541 agtaaacacg ttgacgttga ataccgcttc tctgccgagc cttatattgg tgcctcatgc
      601 agtaatgtgt cagttttatc tatgttatgc ctgcgggcga agaaaacaat ctaaggaatt
      661 tttcaaatgg caaagattaa aggtcaggtt aagtggttca acgagtctaa aggttttggc
      721 ttcattactc cggctgatgg cagcaaagat gtgttcgtac acttctccgc tatccagggt
      781 aatggcttca aaactctggc tgaaggtcag aacgttgagt tcgaaattca ggacggccag
      841 aaaggtccgg cagctgttaa cgtaacagct atctgatcga atccactgat ctgaagtgtg
      901 aatacgcttc aatctcgcta taaagcctcg tcgaatgcga ggctttttac tatgctttat
      961 cttcgctcct ggcgttcgga tatttgcccg ccgcgtgatt cgcgttacac ttgcggcctt
     1021 tagtatcctg ccggagttgt catgtcgttt tcctgtccac tttgccatca gcctctttcg
     1081 cgtgaaaaaa acagctatat ctgtccccag cgacatcagt ttgatatggc gaaagaaggg
     1141 tatgtcaatc tgctgcccgt tcagcataaa cggtctcgtg atccgggcga cagcgcggaa
     1201 atgatacaag cacgccgcgc attcttagat gccggacatt atcagccgct gcgtgatgca
     1261 attgtcgccc aactgcggga acggcttgat gaaaaggcca cggcggtgct ggatattggc
     1321 tgtggtgaag ggtattacac acacgcattt gccgatgtgt tgcccgaaat caccacgttt
     1381 ggcctggatg tctcgaaggt agcgataaaa gcggcggcga aacgctatcc gcaggtcact
     1441 ctttgtgtcg cttccagcca ccgtttgccg ttttccgata ccagtatgga cgccataata
     1501 cgtatttacg cgccgtgtaa agcagaagaa ttagcacgag tagtgaagcc cggcggctgg
     1561 gtcattactg ccacgccggg accgcgacat ttgatggagc tgaaggggct gatttacaat
     1621 gaagtacatc ttcatgcacc tcatgcagaa caactggaag gttttacatt acagcagagt
     1681 gatgagttgt gttatccgat gcgtcttcgc ggtgatgaag ccgtcgcatt attgcagatg
     1741 acgccgtttg cctggcgtgc gaagccagaa gtctggcaaa cactggcagc aaaagaagtg
     1801 ttcgactgcc agacggactt taatattcac ctctggcagc gttcttatta accgtggaag
     1861 tgcgtccaga ggatctggac gccgatgccg atcagcacca gcccgccgag aatttccgct
     1921 tttttcccaa taattgagcc gataaagcga ccaaccatca tccctaatgt cgacataatc
     1981 aaggttgcac aaccaatggc caatgcggtc gcgataatgt tgacctgcag gaaagcaaga
     2041 ccaacaccca cagccatggc atccaggctg gtggcaatcg cggtggttac cagtagccag
     2101 aaaccgtgcc gacggcgcgg ctcttcatct tcatcatctg cgccacgaaa accctcaata
     2161 atcattcgcc cgccgaggaa tatcagcagc acaaacgcaa tccagtggtt ccattcaagg
     2221 acaaaccggc tggctaacat gcccattccc cagccgatca gcggcgtcag ggtttcgacg
     2281 gcaccaaaaa taaggccggt tcgcaatgct tcagaaaatt tcggtttatg gagggtggca
     2341 cctttaccga ttgatgcagc aaatgcatcc atcgacatac caaacgcaag aagaacagta
     2401 gcagtgatat tcatgacaat gtcctgaccg gggtatccat aaagcacatc atcgccccca
     2461 gcaaacatgc atacatgaac aacaagccgc gatggttttc gcgttcatgc aggcatagcg
     2521 attgatgtgc atatggtctc gcctgactga aagtattcag tccgtacgtg ccacgttttg
     2581 caacgagtat gttgacacgt acatttccgg aatctggaaa tcggctactc cccaaatgag
     2641 ggcgcaacct taacatattt tgagaatata aaacaactgt tggaatatat tattt
//
LOCUS       DAZFXT010000267         2667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.269, whole genome shotgun sequence.
ACCESSION   DAZFXT010000267 DAZFXT010000000
VERSION     DAZFXT010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.269"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..122)
                     /locus_tag="ACHKW0_005042"
     CDS             complement(<1..122)
                     /locus_tag="ACHKW0_005042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477686.1"
                     /translation="MGKHHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVT"
     gene            complement(160..360)
                     /locus_tag="ACHKW0_005043"
     CDS             complement(160..360)
                     /locus_tag="ACHKW0_005043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/CI family transcriptional regulator"
                     /protein_id="HFO7477687.1"
                     /translation="MTTDDIESYFGSIEKVAAFFGITTEAVYQWRNRPGQLIPKGRAA
                     EAAYRTCGRLPFKPELYEKSNG"
     gene            458..1084
                     /locus_tag="ACHKW0_005044"
     CDS             458..1084
                     /locus_tag="ACHKW0_005044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family protein"
                     /protein_id="HFO7477688.1"
                     /translation="METVGQRIKALRRVTGTSQKELGKFCGVSDVAVGYWEKDINTPG
                     GEALSKLAKFFNTSIDYILYGAEFEGKLVTNMRRVPVISWVQAGQFTECRAAEVFSEV
                     DKWVDTSLKIGDNSFALEVKGDSMTNPNGLPTIPEGATVIVDPDAEPRHGKIVIARLD
                     GTNEATVKKLVIDGPQKFLVPLNPRYPNIPINGNCLIIGVVKGVQYEL"
     gene            1291..1566
                     /locus_tag="ACHKW0_005045"
     CDS             1291..1566
                     /locus_tag="ACHKW0_005045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001547989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477689.1"
                     /translation="MKVQILNNNCEVVWSYDIAAPVDQSGDSWTNGKHQIMAGVVFSL
                     RRALEQAEVFPSDPEWKWPFSICPNSESTFQKIGQKVALEEHQPTVS"
     gene            1842..2152
                     /locus_tag="ACHKW0_005046"
                     /pseudo
     CDS             1842..2152
                     /locus_tag="ACHKW0_005046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518763.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2167..2529
                     /gene="yfdP"
                     /locus_tag="ACHKW0_005047"
     CDS             2167..2529
                     /gene="yfdP"
                     /locus_tag="ACHKW0_005047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdP"
                     /protein_id="HFO7477690.1"
                     /translation="MASERSTDVQAFIGELDGGVFETKIGAVLSEVASGVMNTKTKGK
                     VSLNLEIEPFDENRVKIKHKLSYVRPTNRGKISEEDTTETPMYVNRGGRLTILQEDQG
                     QLLTLAGEPDGKLRAAGH"
     gene            2594..>2667
                     /locus_tag="ACHKW0_005048"
     CDS             2594..>2667
                     /locus_tag="ACHKW0_005048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477691.1"
                     /translation="MSQNLDATAINQIHALISAQGVNE"
BASE COUNT          751 a          581 c          613 g          722 t
ORIGIN      
        1 tctgttacat ccagccagtc agctgcttca gcgtaaccac ccggcaacgc tgcgatagtt
       61 tttctgacag ctttcacgta ccactcaggc tgtttttcta ctttccagtg atgcttaccc
      121 acggttagcc tcatcgttct gtggtttctg ttaatcgatt tatccattag atttttcata
      181 aagctcaggt ttaaatggca accgtccgca agttctatat gcagcttctg ctgcacgtcc
      241 ttttggaatt aactggcccg gacggtttcg ccactgataa acggcttcag ttgttatgcc
      301 gaaaaaagca gcaactttct caatactgcc gaagtagctt tcgatatcgt cagttgtcat
      361 acgccctcca aactaagttt tattagatgc taattacaaa tctatctttg gtcaataaaa
      421 actaagatta cttagcaatt caagaaatgg tgctcctatg gaaacggttg gtcagcgtat
      481 aaaagctctg agaagagtta ccggaacgtc ccagaaagaa ttgggtaaat tttgtggagt
      541 aagcgacgtt gctgtggggt actgggagaa agacatcaat acccctggtg gggaggcact
      601 ttcgaaatta gcgaagttct tcaatacgtc aatagattac attctttatg gtgctgagtt
      661 tgaaggcaaa ctcgtcacaa acatgcgcag agttcctgta atatcgtggg ttcaggctgg
      721 gcagtttact gagtgcaggg cagcagaagt gtttagtgaa gtggacaagt gggtagatac
      781 atcattaaag attggtgata actcatttgc attagaggtt aaaggtgact ccatgactaa
      841 ccctaatggc ctcccaacaa taccagaagg cgcaacagtg attgtagatc cagatgcaga
      901 acctcgtcat ggaaaaatag tcatcgctcg acttgatgga acaaacgaag ctacagtaaa
      961 aaaattagtc atcgatggcc ctcaaaagtt tttagtgcca ttaaatcctc ggtatcccaa
     1021 catccctatc aatggtaatt gccttatcat tggtgtagtc aaaggagttc aatacgaact
     1081 ctaagacctc tcttctctaa ctaaggcacc gaactaagaa aagtttggtg ttttctcttg
     1141 ccataataac taagttaagt tagattttat atcaaagata acgaacaggc aggacgccca
     1201 cgaagtagcc gcctggggca tatgaagtcc aggatgattc gttgagtcat gttgtgccat
     1261 caggcactca tgttaaagca ggtgtatgaa atgaaagtcc agattttaaa caataactgt
     1321 gaagtcgttt ggtcatacga catagccgcc cctgtagatc agagcggcga tagctggacc
     1381 aatgggaaac atcagattat ggctggagtt gtgttctctt tacgccgtgc tttggaacag
     1441 gctgaagtat ttccatcaga ccctgaatgg aaatggcctt tttctatttg tccaaattcg
     1501 gagagcacat ttcagaaaat tggtcagaaa gtcgcactcg aagagcatca gccaactgtt
     1561 tcctgatttt ttcaggtaac tcgtcggcat cgcagaaaca acaacgctcg atcatgttga
     1621 aagccgattc gtagaaccgt ttctgctgag tgtcgctgag acaggaaaag agtgacgtta
     1681 cgatgatttt attaattgca ttatcaagtt ctttttcatc aaaagtcatt tgattttcct
     1741 tttatgtata cgggcttaaa aggataccac cgagcctgaa gtggtgaaaa gacaggcaca
     1801 taacagctaa gtattttcaa ccaaagagaa tccttagcgt tgtggtgaat gcggctcagc
     1861 gcacgcgggt taaggttgag gctgacagtc gaccttctgt ggatacccac ccgtctggtg
     1921 tgcaaccttc gccaggcacc gggaggcacc cggcaccaca actttatgct gtgtgtagtc
     1981 ctggcggtac cagtttgtac ccttgcttcc ggctggtagc gtccttttta caaaacagag
     2041 aagagcatca ccggacgacg ggctcataac ccaatccatc cgggcggctg ccaccgcagg
     2101 tgttcttctc tgttttgtgg agaaactaat cggccttgca gggtcgatat gatgaggagc
     2161 agcaaaatgg ctagcgaacg cagtactgat gtgcaggcat ttatcgggga gctggacggc
     2221 ggcgtatttg aaaccaaaat cggcgcagtt ctcagtgaag tcgcttccgg tgtgatgaac
     2281 acgaaaacca aaggtaaggt ctcactcaac ctggaaatcg aaccatttga tgagaaccgt
     2341 gtgaaaatca aacacaaact ctcatatgtt cgcccgacta accgcgggaa aatttctgaa
     2401 gaagacacca ccgaaacgcc gatgtatgtc aatcgcggtg gtcgcctgac tattctgcag
     2461 gaagaccagg gacaattact gactcttgcc ggtgaacctg acggaaaact ccgcgcagca
     2521 ggtcattaat atcgttttta attaactgat tatttatctc atcactgaat attttatata
     2581 gtgaggactt attatgtctc agaacttaga cgcaaccgca attaatcaaa tccatgccct
     2641 tatttctgct cagggtgtta atgaaat
//
LOCUS       DAZFXT010000268         2591 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.271, whole genome shotgun sequence.
ACCESSION   DAZFXT010000268 DAZFXT010000000
VERSION     DAZFXT010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2591
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.271"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(3..483)
                     /gene="ydcD"
                     /locus_tag="ACHKW0_005049"
                     /pseudo
     CDS             complement(3..483)
                     /gene="ydcD"
                     /locus_tag="ACHKW0_005049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415974.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdcD"
     gene            complement(467..>2591)
                     /locus_tag="ACHKW0_005050"
     CDS             complement(467..>2591)
                     /locus_tag="ACHKW0_005050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7477692.1"
                     /translation="WHYDASDRITHRTVNGDPAEQWQYDEHGWLTTLSHTSEGHRVSV
                     HYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPPVEWLTYG
                     SGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQLQSQHLN
                     SLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAG
                     NRLPDPELHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDRIPTGVIRTDDERTH
                     HYHYDSQHRLVFHTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPE
                     ETWYGWDGDRLTTVQTDTTRIQTVYQPGSFTPLIRVETENGEREKAQRRSLAEKLQQE
                     GSEDGHGVVFPAELVGLLDRLEGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLP
                     ERKIHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYD
                     KESGLYYNRHRYYDPLQGRYITPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTS
                     GGLIYAVSGVPGLIAANSITNSAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTF
                     GSTIADVIGKNHEAAGDRQGQPAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQ
                     LFGLDGIKADNPIKAKQGSSDASNY"
BASE COUNT          529 a          770 c          593 g          699 t
ORIGIN      
        1 cattatgacc atttaccaat ccagtcaaaa acttcatttt tcaaatcgtt atcataataa
       61 tcaacatcgt aatagtctcc ataattaatt attttataac tagccttctt gtttttatca
      121 ctaaaataca cccgttgttt tttagcgcag aactccatat tctccagttt actaatgttt
      181 tttaatcgca cgctataaag aatatcattg aaaaaattaa gatttatatc aaccactcca
      241 cagtgcgtcc tgtaattatt aaccattata cttataaaat gaccattccc tctactaatt
      301 ttttggtcat taattatttt ataaataata tttttcagaa agatatatct caacgctctt
      361 tggtgtttta tgtccataaa atacgatgtc agaatttgaa ttgttatcaa acccaaggta
      421 aacaacaaat aaacaaaggg taattaaaaa aatgacaagt ttgcctctaa taatttgaag
      481 catctgaact cccttgcttt gcttttattg gattatctgc ttttatacca tctaacccga
      541 agagttgccc agtgttatat ttttcaatac atgcatcgct acatttggca gaaaaatcgc
      601 cacaagcaat accgacagtg ttatttttaa gatccatgat tctttcttta gctggctgac
      661 cttgtctatc ccctgccgcc tcatgatttt tacctatcac gtcagctatt gttgatccaa
      721 atgtcttagt cattcgacac atcaagtaac aatgtctcat tgcatcggcg gccccattat
      781 gagctccgcc aacaatagca tccatatcat aaccgaactg gtaagcactg ttagtaatgc
      841 tgtttgcagc aatcaatcca ggtacaccag atacggcata aattagtcca cctgatgtca
      901 tattctcaat cgcatctaac cccattgggt ctatcacttg tatgggattc aacggatact
      961 gatacatatt ccatccacct ctcaacccaa tcgggtccgg ggtgatatac cgcccctgca
     1021 acggatcgta gtaccggtga cggttatagt acagccccga ctccttatca tactgctgcc
     1081 ccggcagccg gtacggctgg tgcaggtgat gcgggttctc ctcattaagc tggttgcccc
     1141 attcatcata ttccgcgctc cacgccgtat tgccgtcttc actgataagc gccagcggca
     1201 ggccccggtg gtcacagtga taaagatgta tttttcgctc aggcaaataa accggctcta
     1261 tctgtgccgc cagccgctcc accgtcagcc cgcactgcgc aagccactgg cggctttcac
     1321 tgctcacgca gtttgcccgg atttccccct ccagcctgtc cagcagcccc accagttcag
     1381 ccgggaacac cacaccgtga ccgtcttcgc tcccttcctg ctgtaacttc tccgccaggc
     1441 tgcggcgctg cgctttctcc cgctcgccgt tctctgtctc gacccggatg agcggcgtga
     1501 agctccccgg ctggtatacc gtctggatac gcgtggtgtc ggtctgtacc gtcgtcagcc
     1561 tgtcgccgtc ccagccgtac catgtctcct ccggtttacg cgacagcgac atccaccccg
     1621 tcaggtcacg ctcccgccgc catacccgtt tcgccattcg ccgtcccagc gggtcgtaga
     1681 ggtagcggct ctcgaccagt ggctcgccat gctgtatccg cgtgtggaac accaggcggt
     1741 gctggctgtc gtagtggtag tggtgggtcc gctcgtcgtc cgtccgtatc acacccgtcg
     1801 ggatacggtc cgtcttctcc gtcagtctgc cgtattcatc atagtggtaa acatagtgcg
     1861 catcctctgc gatgcggtta tccggccacg ccgtgagcgt gctgtccggg tgtagctccg
     1921 ggtccggcag gcggtttccc gccgggtcgg tggcatacgg gatgcggatg tccaggtccg
     1981 gtgcgagggt gcgcacgctc tccagcctgc ccgtggcgct gtagccgtat tcccgcgtct
     2041 gtcgcggacc gctgatgcgc accaggtcgc cgttgtcatt ccacccgtag tcacggtcat
     2101 ataccaggct gttcaggtgc tggctctgta actggcctgc gggggtgtat gtgctggtca
     2161 gttcgtatgc ggcattactg cctgccctgc tgccgaagct gcgcaccgtc tcacggtgca
     2221 gcctgtcccg cgtatactcg accagcggcg tcccgcccag cttcatgccc gccaggtaac
     2281 cgctgccgta ggtcagccac tccaccggcg gcaggctgtc cggcgtgacg cggtttgcca
     2341 gcccctgttc gttgtaggcg tgtttcgtct catgctgcca cagcagttcc cccgtctccg
     2401 ggttctccac cgtctgccgt tcgcccgtca ggcggccctt atcgtcgtag ccgtagtgga
     2461 ccgacacgcg gtggccttca ctggtatggc tgagggtggt taaccacccg tgctcatcat
     2521 actgccactg ctctgccggg tcgccgttca ccgtccggtg cgtgatgcgg tccgacgcat
     2581 cgtagtgcca g
//
LOCUS       DAZFXT010000269         2566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.272, whole genome shotgun sequence.
ACCESSION   DAZFXT010000269 DAZFXT010000000
VERSION     DAZFXT010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.272"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(13..438)
                     /locus_tag="ACHKW0_005051"
     CDS             complement(13..438)
                     /locus_tag="ACHKW0_005051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin YdaT family protein"
                     /protein_id="HFO7477693.1"
                     /translation="MKIKHEHIRMAMNAWAHPDGEKVPAAEITRAYFELGMTFPELHD
                     DSHPEALARNTQKIFRWVEKDTPDAVEKILALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(422..703)
                     /locus_tag="ACHKW0_005052"
     CDS             complement(422..703)
                     /locus_tag="ACHKW0_005052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdaS family helix-turn-helix protein"
                     /protein_id="HFO7477694.1"
                     /translation="MEELRIFLNSLSSDEQRMFACECGTSIGYLRKALSKGQVLGASL
                     CVLIERASNGEVTRQQLRPFDWMNIWPELEDTKTLTQPLSRSLIHENQA"
     gene            804..1223
                     /locus_tag="ACHKW0_005053"
     CDS             804..1223
                     /locus_tag="ACHKW0_005053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477695.1"
                     /translation="MDKYEFRRQQLIKIRDEKCDGKAVNVARKIGREPSYVSRMLYPE
                     GKKGKKRIADDMVEIIEESFGLPRGWMDGIVSSSTNTASSYETRVLTPRQRIFLDLLD
                     ELPESEADNLLKTLEEKKQYYNMIYEEIRKKKAQNAS"
     gene            complement(1628..1828)
                     /locus_tag="ACHKW0_005054"
     CDS             complement(1628..1828)
                     /locus_tag="ACHKW0_005054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477696.1"
                     /translation="MNTIKFSCPECGGEVFDTSFKPHGSDSFAGAICKNCGHLVTEDE
                     SSQFDDEIVDNIFGALTRDFLK"
     gene            1921..2139
                     /gene="ydfC"
                     /locus_tag="ACHKW0_005055"
     CDS             1921..2139
                     /gene="ydfC"
                     /locus_tag="ACHKW0_005055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdfC"
                     /protein_id="HFO7477697.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMHQKIKAAQDLKWANEARAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
BASE COUNT          729 a          610 c          599 g          628 t
ORIGIN      
        1 ggctattatc actcaatgca ctgccacagc atttcctgcc gggccaccac ggttcatctg
       61 attgaaaccg gcgattgcca ccgcgacaaa atcatcagcg tctctcacca gtcgctcccg
      121 cgtctccacc agctcccgaa aataagctga actgtggctg cgcattctgg ccaccagcaa
      181 aggtggcatt gccttttcga tcgctggtaa caacgccaga attttttcaa ctgcatcagg
      241 ggtgtctttc tctacccagc ggaaaatttt ctgggtattg cgagccaggg cttccggatg
      301 gctgtcgtca tgcagttctg ggaacgtcat acccagttca aaataagccc tggttatttc
      361 agctgccgga actttttcac cgtccggatg cgcccaggca ttcatcgcca tgcggatgtg
      421 ttcatgcttg attttcatga atcaagctcc tagaaagtgg ttgtgttaac gttttggtat
      481 cttccagctc gggccaaata ttcatccaat caaaaggcct tagttgctga cgtgtaactt
      541 caccattact ggctcgctca ataaggacac ataacgatgc ccctaacact tgacctttac
      601 tcaatgcctt tcttagataa ccgatgctgg taccacactc gcatgcaaac atacgctgtt
      661 catctgacga aagagaattg agaaatattc ttaattcttc catagctact ccttagtaaa
      721 cacagcaaag aatacctaca ggtaaacaaa agtcaatacc cacaggttgt ttaccttgca
      781 gtaatcgcat ctattattta cctatggaca aatatgaatt tagacgacag caactcatca
      841 aaattcgtga tgagaaatgc gatggtaaag cggttaacgt ggccagaaag atcgggcgcg
      901 agccttctta tgtatcaaga atgttgtacc cagaggggaa aaagggaaaa aaacggatcg
      961 ctgatgatat ggtggagatt atcgaagagt cctttgggtt accccgggga tggatggatg
     1021 gtatcgtttc atcatcaacg aacacagcct ccagttatga aacaagggtt ctaacgccac
     1081 gacaacgtat ttttttagat ctcttagacg aactgccaga aagtgaagcg gataacttat
     1141 taaaaactct tgaagagaaa aaacagtatt acaatatgat ctacgaagaa atccgtaaaa
     1201 agaaagcaca aaacgcatca tagctcacca aacaactagt caccagttaa gacaccgcaa
     1261 aaagttaccc atgggtattt actttttaaa tacctatggg tatccttctt ttcataccaa
     1321 ctcaccccgc tccacagaat gcagggcaat acttcgagtt atcaggcagt ggtcaggggt
     1381 taagtagcca gcccgaggcg taagaacatg acggcagggt tcaactttaa taactatgca
     1441 gcaggttttt gttccgctac cccagcgtta aggggaaaca gaggatttct cagtgggcga
     1501 agtcaaacat cagaatggaa ggcatccagg gatcagcaaa gaaacagcga tggcgcttta
     1561 tattgatatc agcgccattg ccggacaggt aagaattatc agagcggtaa ctaagcggta
     1621 tgcgccttta cttcagaaag tctctggtga gtgcaccgaa gatattgtca acgatttcgt
     1681 catcaaactg cgaggactca tcttcagtta caaggtgacc acaatttttg cagatggctc
     1741 ccgcgaaact gtcagagcca tgcggtttaa aggatgtgtc aaagacttcg ccgccacatt
     1801 ctgggcaaga aaacttgatt gtattcataa ccaatttcct ctcgagtaac agacccctca
     1861 gaggatacca cctcgcctga cgtggttaaa agcaggcaac gctaaccaca aggagccgac
     1921 atgcagaaac gagaacccgt catcatcgcg ccagactata ccgatgatga actttatgag
     1981 tggatgcacc agaaaattaa ggcagcgcag gacctgaaat gggccaatga agccagggct
     2041 aagcaagctg aaaatctgtc cgctctggag caggatatca ccaatctgga aaaagcagcg
     2101 gcattaagca ttgccagaat gattacatac ccgcgttaat agctaaccaa cgaagctaag
     2161 gttggtaatt aaggagttct ccacgggtga ggtggagtgc gtgcgccgga cacgggtgag
     2221 catccggcac tgacagttta ctgaaaggat atttccctga aaagtcaggg cataacgcga
     2281 aagcgcacgg cgaggtagct ggttcataga tagcctgtcg ttaaattttc gtcgaccgtg
     2341 cgcttccggt tgtggcactc cgcgaaatgg cgcggcggta agtatggcgg gattattcct
     2401 tccccgttga ggacaccggg ttgtcaggtt gaccatacgc ttaagtgaca accccgctgc
     2461 aacgccctct gttatcaatt ttctggtgac gtttggcggt atcagtttta ctccgtggct
     2521 gctctgccgc cctttttaaa gtgaattttg tgatgcggtg aatgcg
//
LOCUS       DAZFXT010000270         2470 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.273, whole genome shotgun sequence.
ACCESSION   DAZFXT010000270 DAZFXT010000000
VERSION     DAZFXT010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.273"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..463)
                     /locus_tag="ACHKW0_005056"
     CDS             complement(<1..463)
                     /locus_tag="ACHKW0_005056"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO7477698.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSAIPFLWQHDHSRPVGQCTVRRVSEGLEITATLVKPVPDMPSQLAARLDEVWAAIK
                     TGLVRGLSVGFRPHEYTFLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKS"
     gene            complement(527..2188)
                     /locus_tag="ACHKW0_005057"
     CDS             complement(527..2188)
                     /locus_tag="ACHKW0_005057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7477699.1"
                     /translation="MIPVWSTACPDWAERLKKGLSIIPDPIYPDEAAHALAIFKQLRI
                     VDAPGSPTFGESCAPWVFDLVAALFGSYDAQTGVRHIKEVFILIPKKNSKSTLAAGIM
                     MTALLLNWRQAAGYTILAPTVEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRV
                     TDTTLKVVAADPNTVSGIKSVGTLIDELWLFGKQCKAEDMLREAIGGLASRPEGFVVY
                     TTTQSNEPPAGVFRQKLQYARDVRDGKIHDPHFLPVIFEHPPEMVESGANLLMENLAM
                     VNPNLGYSVDEAFLYREYRKAREAGEETFRGFMSKHANVEIGLALRSDRWAGADFWEE
                     QGRCISLDDILRRADVVTVGIDGGGLDDLLGMYVIGRDRETREWLGWGHAWAHETAVV
                     RRKSEASRFQDLVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQIL
                     DSLAEAGIPDGIVVGISQGWKLGGAIKTTERKLAEGVLVHGDQPLMAWCVGNARVEPK
                     GNAILITKQASGRGKIDPLMALFNAVSLMSLNPEPKKKEYAVFFI"
     gene            complement(2185..>2470)
                     /locus_tag="ACHKW0_005058"
     CDS             complement(2185..>2470)
                     /locus_tag="ACHKW0_005058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HFO7477700.1"
                     /translation="EMPPSAVMSPGIEYMEDGLPDPVKAMGRILVENLCIDPKLALDA
                     AWRLAQFTHHKKGDTGKKSAKGDAAKKAANRFAVPPPPRLVVNNDNEGNG"
BASE COUNT          527 a          805 c          622 g          516 t
ORIGIN      
        1 aagatttaat ggtccggatg gtgcattccg cattcgcggg cacggtgacg gcagacacct
       61 ccatcagttc ccagcgcaga aaatgcagtc cgcctccgtc cagaaaggtg tattcatggg
      121 gacggaagcc cacggacagc cccctgacca gcccggtctt aatggccgcc cagacctcat
      181 ccagccgggc agccagttgc gacggcatat ccggtacggg cttcaccagt gttgccgtga
      241 tttccagccc ttcgctgacc cggcgcaccg tacactgccc caccgggcgg gaatggtcat
      301 gctgccagag aaacggtatc gcactgccaa actccgcgcc ctccggctcc aggatgtcac
      361 catcccgatc cggagaaggc gttgacgcaa tcccggtgat cacccgttca tcctcactga
      421 aggatttcac cgtcagcagg gaacaggccc gtttaagagt cacatcagcc tcctgaaaat
      481 aaaaaaaccg ccggagcggt tcgtgatggt tacagtgtga acagggttat atgaaaaaaa
      541 ccgcatattc tttctttttc ggttccggat tcagggacat cagggagacc gcattgaaca
      601 gcgccatcag cgggtcaatt tttccccgtc cactggcctg tttggtgata agaatggcgt
      661 tacctttagg ctccacccgg gcattgccga cacaccaggc catcaggggc tggtcaccat
      721 gcaccagcac cccttcagcc agtttgcgct cggtggtttt aatggccccg cccagtttcc
      781 agccctggct tatccccacc acaattccgt cggggatccc ggcttccgcc agtgaatcca
      841 gaatctgccc cacccctgac gggtcaatac cgatatggtc cagtaactca gcctcatgaa
      901 tgcgacgcac atattccgcc acttccgccg tgtcatcccc gacacgccgg acaatggtca
      961 tatctccaca ggcaacaaga tcctgaaacc gggacgcctc gctcttccgt cggaccaccg
     1021 cggtttcatg cgcccaggca tggccccagc ccagccattc gcgggtctcc cggtcacgcc
     1081 caatcacata catccccagc agatcatcca gccctccgcc gtcaatcccc accgtcacca
     1141 catcagcacg acgcaggata tcgtccaggc tgatacaacg gccctgctct tcccagaaat
     1201 cagcccccgc ccagcggtca gagcgcaggg caagaccaat ttccacattg gcgtgttttg
     1261 acatgaaccc ccggaatgtc tcttcaccgg cttcccgggc tttacggtac tcccggtaca
     1321 gaaaggcctc atccactgaa tagccgagat tcggattgac catggcgagg ttttccatca
     1381 gcaggttagc cccgctttcc accatttcag gagggtgttc aaatatcacc ggcagaaagt
     1441 gcggatcatg aattttgccg tcgcgcacat cccgggcgta ctgcagtttc tgtctgaaca
     1501 ccccggcggg cggttcattc gactgggtgg tcgtatacac cacaaaccct tccgggcggg
     1561 aggcaaggcc gcctatggct tcacgtaaca tgtcctccgc cttgcactgc ttgccaaaca
     1621 gccacaactc atcaatcagc gtacccacgg acttgatacc ggacaccgta ttcggatcgg
     1681 ctgccaccac cttcagggtg gtgtccgtca cccggtgggt gatggtccgg atatgggtct
     1741 gtacctggca gaggtcatcc agatcatcgt cacgtcgtac catatcccgg gcagggttga
     1801 aggcgttggc cgccacctcc acagtcgggg ccagaatcgt gtaacccgcc gcctgccgcc
     1861 agttcagtaa cagtgcagtc atcatgatcc ccgcagccag cgtggacttc gagtttttct
     1921 tggggataag gataaaaact tccttgatat ggcgtacacc ggtctgcgca tcgtaggagc
     1981 caaacagggc cgccaccagg tcaaacaccc acggtgcaca ggactccccg aacgtagggc
     2041 taccaggtgc atccacaatc cgcagttgtt taaaaatcgc cagggcatgt gcggcctcgt
     2101 ccggataaat cggatccgga ataatcgaca gccccttttt caggcgctct gcccagtccg
     2161 ggcaggctgt gctccataca ggtatcatcc gttgccctca ttatcattat tcaccaccag
     2221 tcggggtggc ggtggcaccg caaaacggtt agccgctttt ttcgcggcat caccttttgc
     2281 cgattttttc ccggtatccc cttttttatg gtgcgtgaac tgcgccagac gccaggccgc
     2341 atccagtgcc agtttcggat caatgcagag gttttccacc aggatccgcc ccatggcttt
     2401 caccggatcg ggaagaccat cctccatata ttcaatacca ggagacatca ccgcggacgg
     2461 tggcatctcc
//
LOCUS       DAZFXT010000271         2465 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.274, whole genome shotgun sequence.
ACCESSION   DAZFXT010000271 DAZFXT010000000
VERSION     DAZFXT010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.274"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            65..499
                     /locus_tag="ACHKW0_005059"
     CDS             65..499
                     /locus_tag="ACHKW0_005059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7477701.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
     gene            496..846
                     /gene="tnpB"
                     /locus_tag="ACHKW0_005060"
     CDS             496..846
                     /gene="tnpB"
                     /locus_tag="ACHKW0_005060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7477702.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQIQHVLDDNPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            877..>2465
                     /locus_tag="ACHKW0_005061"
     CDS             877..>2465
                     /locus_tag="ACHKW0_005061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
                     /protein_id="HFO7477703.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEALLKQREQESDRYS
                     GREDDPLVPRQLRQSRHRRPLPAHLPREIYRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPLNDALYSYVMNTRKVHTDDTPVKVLA
                     PGRKKAKTGYIWTYVRDDRNAGSPEPPAVWFAYSPDHQGKHPEQHLRPFRGILQADAF
                     AGYDRLFSAEREGGALTEAGCWAHARRKIHDVYISTKSATAEEALKLIGELYAIEHEI
                     RGLPVSERLAVRQMQSKPLLTSLYKLMQEKEHTLSKKCRLRDAFRYIRKHWVALCNFC
                     DDGLAEADNNTAERALRAVCLGKKNYVFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLP"
BASE COUNT          609 a          635 c          680 g          541 t
ORIGIN      
        1 tctgtaacac ctgctccttg cagactaaat tagagctcct tctaaattag acggagttct
       61 attgatggat aaacctacag actggcgctc cggaacccgc cggatatttt ctaatgaatt
      121 taaacttcat atggttgaac tggcttcgaa accaaatgcc aatgttgcac aactggcccg
      181 ggaacatggc gttgataaca acctgatttt taaatggcta cgcctctggc aaagagaagg
      241 acgtatttct cgtagaatgc ctccaactat tgtaggccct acagtatcac aatcttttcc
      301 ggcctctccg actctggttc cagtggaact tatcgacacc ccgcgctgtg ctacagatgc
      361 tcctgctccg gaggcattat cagttgcttg tgcagcttcc tgccatgtgg aattccatta
      421 cggtaaaatg atgctggaaa atccttcacc agagctgctc acggtgttga tccgtgagct
      481 gaccgggagg ggacgatgat ttcactccca tcaggtaccc gtatctggct cgttgccggc
      541 gttactgata tgcgtaaatc cttcaacggt ctgggggagc agatacagca tgtgctggat
      601 gataacccct tctccggtca cctgtttatc ttccgtggcc gacggggaga cacgattaaa
      661 atcctgtggg ctgatgctga tggtctgtgc ctgttcacca aacgccttga ggaaggtcag
      721 tttatctggc ctgcggtgcg tgacggtaag atatccatta cccgctcgca actggcaatg
      781 ctcctcgata agctggactg gcgtcagcca aaaacatccc gccttaacgc actgacaatg
      841 ttgtaaaaaa ctcctgaccg cattataaaa acggccatga gtcagaaata cctcattcgc
      901 atcgctgagc tggaaaggct gctctctgag caggctgaag ccctccgtca gaaagaccag
      961 caactgagtc tggttgaaga gacggaggcc ttcctgcgct ctgcactggc acgtgccgaa
     1021 gaaaagatcg aagaagatga acgggaaata gagcatctgc gggctcagat agaaaaactg
     1081 cgccggatgc tgttcggaac ccgttctgaa aaactgcgtc gtgaggttga acaggctgaa
     1141 gccctgctga aacaacgcga gcaggaaagc gatcgttaca gtgggcgtga ggatgacccg
     1201 ctggttcccc gccagttgcg acagtcgcgc catcgtcgtc cgttaccggc acatctcccc
     1261 cgtgaaatat accgcctgga gcctgaagaa agctgttgcc cggagtgtgg cggtgagctg
     1321 gattatctgg gggaagtcag cgcagaacaa ctggaactgg tgagcagcgc cctgaaagtg
     1381 atccgcacag aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg
     1441 gcaccatccc gtccgataga gcgtggtatc gcgggcccgg ggttacttgc ccgcgtgtta
     1501 acgggaaaat actgcgaaca cctgccactg tatcgtcaga gtgaaatctt tgcccgccag
     1561 ggtgtcgaac tgagccgtgc cttactctcc aactgggttg atgcgtgctg ccagttaatg
     1621 acgccgctga atgatgccct gtacagttat gtgatgaaca cccgcaaggt tcacactgat
     1681 gacacaccag taaaagtact ggcaccgggc aggaagaagg cgaaaacagg atatatctgg
     1741 acgtatgtcc gggatgaccg aaatgccggt tcgccagaac ctccggcggt ctggttcgcc
     1801 tactcaccgg accatcaggg taaacatccg gagcaacacc ttcgtccctt ccggggtatc
     1861 ctgcaggcag atgcgttcgc aggttacgat cggctgttca gtgccgaacg tgaaggcggc
     1921 gcgttgacgg aagcaggatg ctgggctcat gcgcggcgca aaatccacga tgtatatatc
     1981 agtaccaaaa gcgcgacggc ggaagaagca ctgaaactaa tcggcgaact gtacgccatt
     2041 gagcacgaaa tacgcgggtt gccggtgtct gaacgcctgg cggtcaggca aatgcagagt
     2101 aaaccgctac tgacttccct gtataagctg atgcaggaga aagaacacac gttatcgaaa
     2161 aaatgccgtc tgagagatgc gttccggtat atcaggaagc actgggttgc gttgtgcaac
     2221 ttctgtgatg acggtctggc ggaggcggac aataacacag cggaaagagc gcttcgtgca
     2281 gtctgtctcg gaaagaaaaa ttacgtgttc ttcggtagcg atcacggcgg cgagcgtggt
     2341 gcactgttgt acgggctgat cggcacctgc cggttgaatg gtatcgatcc ggaagcgtat
     2401 ctgcgccata tcctgagcgt actgccggaa tggccttcca accgagttga cgaactcctg
     2461 ccatg
//
LOCUS       DAZFXT010000272         2452 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.275, whole genome shotgun sequence.
ACCESSION   DAZFXT010000272 DAZFXT010000000
VERSION     DAZFXT010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.275"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..141
                     /gene="dicB"
                     /locus_tag="ACHKW0_005062"
     CDS             <1..141
                     /gene="dicB"
                     /locus_tag="ACHKW0_005062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7477704.1"
                     /translation="IGVLLSNREFTEDAIKMRKYEPYLLNDNSILSRIALLELGIIGE
                     QQ"
     gene            138..326
                     /locus_tag="ACHKW0_005063"
     CDS             138..326
                     /locus_tag="ACHKW0_005063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7477705.1"
                     /translation="MTSAFALIMTVFLITGEPQNVITGIYYSKSSCIQVRDEQKIPGE
                     CLPLKKVSLYLNNETPAG"
     gene            419..>2452
                     /locus_tag="ACHKW0_005064"
     CDS             419..>2452
                     /locus_tag="ACHKW0_005064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051908 - double-stranded DNA 5'-3' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0090304 - nucleic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecE family exodeoxyribonuclease"
                     /protein_id="HFO7477706.1"
                     /translation="MNADKEEIALYYEAKNDKVRKRLGIKGGFYWRTAKKLSVAISRG
                     VAAMDDAGFDEEDFKKPIRVHLPVVNDLPPEGVFDTEFCNRYEKGGEDGITMILIAPS
                     VPDKPASTDNTNVNGEDMAKIEENMLLPVSGQILPVRWLAQHNSEKPLTHVSLDKLRA
                     LHNAQDEKLPAVTALAISNKAVQLEPLELRDLYKLVRDTDKVFPAPVNSDLGLMTSFI
                     EAYLGADYTDRGLLTKEWMKGNRVSRISRTPSGANAGGGILTDRGEGFVHDDASVARD
                     VATGVLARSMDVDIYNLHPAHAKRIEEIIAENKPPFSVFFSKFITMPGHKDYSRAIVV
                     ASVKEAPIGIEATPHRVTEYLNKVLTETDHATPDPEIVDIACGRSSAPMPQRVTKEGK
                     QDDEEKPQPSGAMADEQATTEAVEPDTTEHNQDTQSMDAQPQINSVDAKYQKLRAELH
                     EAKKNIPPKNPVDADKLLAASRGEFVEGISDPNDPKWVKGIQSRDDDDQNQDKAEQNS
                     PNALQNEPKTKQPEPVVQQEPEKVCTTCGQTDGGNCPDCGAVMGDATYQETFNEESQD
                     EARKKNPEEMENAVLPNKQCTEGDQHANGNNETGETANPLITVNGHHVITSTSRTCDH
                     LMIDLKTMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTIDLETAGGVIDRDTIK"
BASE COUNT          761 a          582 c          576 g          533 t
ORIGIN      
        1 attggagttc tgctcagtaa ccgggagttt actgaagatg ccattaagat gagaaaatat
       61 gaaccttatc ttcttaatga taattccata ctttccagaa ttgcccttct tgaacttggt
      121 attatcggag aacagcagtg acttcagcgt ttgcactgat aatgaccgtt tttcttataa
      181 cgggtgagcc gcagaatgtg attaccggaa tttattacag taagtcatcc tgcattcagg
      241 taagggacga acaaaaaatc cccggtgaat gcctcccgtt aaaaaaagta tcgctgtacc
      301 tgaataacga aacaccggct ggataacccg ccagccatat taacgccata ccaacggatt
      361 aaaaatgcca gcaatggcag gaatttgttc atccttaaaa tggttatgag gtttatcaat
      421 gaacgctgat aaagaagaaa ttgcactata ttacgaagcc aaaaatgaca aagtcagaaa
      481 acgccttggg attaaaggtg gtttttactg gcgcacagca aaaaaattat cggttgcaat
      541 atcacgtggt gttgctgcaa tggacgatgc cggatttgac gaagaggatt ttaaaaaacc
      601 catccgcgtc catttacccg ttgtgaatga ccttccaccg gaaggtgtgt ttgatactga
      661 attctgcaac cgatacgaaa aaggcggaga agatggtatc acaatgatac ttatcgcgcc
      721 ctctgttccg gacaaaccag ccagtactga caataccaac gtcaacggcg aagacatggc
      781 taagattgag gagaatatgc ttcttccggt ttcaggtcag attctgcctg ttcgctggct
      841 ggcgcaacac aacagcgaaa aaccgctcac gcacgtttca ctggacaaac tgcgcgcatt
      901 acataacgca caggatgaaa aacttcccgc cgttaccgcg ctggccatct caaataaagc
      961 agtgcaactc gaaccgctgg agcttcggga tttgtacaaa ctggtgcgag acactgacaa
     1021 agttttcccc gcccccgtaa attcggacct gggactgatg acctctttta tcgaagcata
     1081 cctgggcgct gactacaccg atcgcggtct gcttacaaaa gagtggatga aaggaaatcg
     1141 tgtttcacgc atcagccgca cgccatccgg cgctaacgct ggcggcggca ttcttaccga
     1201 tcgcggcgaa ggttttgtcc acgatgatgc gtcagtggcg cgtgacgtag ccactggcgt
     1261 actggcccgt tcaatggacg tggatattta taaccttcat cccgcgcacg ctaaacgcat
     1321 tgaggaaatt atcgctgaaa ataaaccgcc attttctgtg tttttcagca aattcatcac
     1381 catgccgggc cacaaggatt actcccgcgc catcgtggtt gcgtccgtga aagaagcacc
     1441 aattggtata gaggctactc cccaccgtgt caccgaatac ctgaataagg tgctgactga
     1501 aaccgatcat gccacccctg atccggaaat cgtggatatt gcctgtggtc gctcctctgc
     1561 cccgatgccg cagcgtgtaa caaaagaagg aaaacaggat gatgaagaaa aaccgcagcc
     1621 atctggcgca atggcagatg aacaggcaac gactgaagca gtggaaccgg atacaactga
     1681 acataatcag gacacgcagt cgatggatgc tcagccacag ataaattctg ttgatgcgaa
     1741 atatcagaaa ctgcgtgcag aactccatga agccaagaaa aacattccac ccaaaaatcc
     1801 tgtcgatgca gataaattac tggctgcttc tcgaggagaa tttgttgaag ggattagcga
     1861 cccgaatgat ccaaaatggg tgaaggggat ccagagtcgc gatgatgatg accagaatca
     1921 ggataaagcg gaacaaaaca gcccaaatgc gttacaaaac gagccaaaaa caaaacaacc
     1981 tgaaccagta gtgcaacaag aaccagaaaa agtttgcacc acatgtggtc agaccgatgg
     2041 cggcaactgc cctgattgtg gagcggtaat gggtgacgca acctaccaag agacattcaa
     2101 cgaagaaagc caggatgaag cccggaaaaa aaatccggag gaaatggaaa atgccgtgct
     2161 cccgaataag caatgcaccg aaggcgatca acatgccaat ggcaataatg aaacaggcga
     2221 gacagcaaat cccttaatta cggtgaacgg tcatcacgtt atcacatcca ccagcaggac
     2281 gtgtgaccat ctaatgatcg accttaaaac catgggaaaa aatcctgatg ccccgattat
     2341 ctcaataggt gcaatatttt tcgatccgca aaccggagat atgggaccgg aatttagtaa
     2401 gactatcgat ctggaaactg ctggcggagt cattgatcgg gacaccatta aa
//
LOCUS       DAZFXT010000273         2448 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.276, whole genome shotgun sequence.
ACCESSION   DAZFXT010000273 DAZFXT010000000
VERSION     DAZFXT010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2448
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.276"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..588
                     /gene="eco"
                     /locus_tag="ACHKW0_005065"
     CDS             79..588
                     /gene="eco"
                     /locus_tag="ACHKW0_005065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004867 - serine-type endopeptidase
                     inhibitor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HFO7477707.1"
                     /translation="MKKESKKMKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAE
                     KGMKRQVIQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFD
                     KVSSPVSTMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAE
                     EKIDNAVVR"
     gene            complement(737..2383)
                     /gene="mqo"
                     /locus_tag="ACHKW0_005066"
     CDS             complement(737..2383)
                     /gene="mqo"
                     /locus_tag="ACHKW0_005066"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008924 - L-malate dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HFO7477708.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
BASE COUNT          587 a          670 c          607 g          584 t
ORIGIN      
        1 ttaagttatt cataaagcaa attaataaat ctgatgaata tgttaacctt cagcgacatc
       61 atcggtgaaa acctataaat gaagaaggaa agcaaaaaaa tgaagaccat tctacctgca
      121 gtattgtttg ccgctttcgc taccacttcc gcctgggcgg cagaaagcgt ccagccactg
      181 gaaaaaatcg cgccttatcc acaagctgaa aaagggatga agcgtcaggt gattcagtta
      241 accccgcaag aagatgaatc taccctgaaa gtagaactgt taatcggtca gacgctggaa
      301 gtcgattgca atttgcatcg tctcggcggg aagctggaaa acaaaacgct ggaaggctgg
      361 ggctatgatt attatgtctt tgataaagtt agctcgccgg tttccacgat gatggcctgc
      421 ccggatggca agaaagagaa gaaatttgtc accgcgtatc tgggcgatgc tggaatgctg
      481 cgttacaaca gcaagctgcc gatcgtggtg tatacgccag acaatgtaga tgtgaagtac
      541 cgcgtctgga aggcggaaga gaaaattgac aacgcggtag ttcgctaaaa tgctgtgaag
      601 tgcggcaccc cgtaggccga ataaggcggt cacgccgcat ccgacatcta acgcccgagc
      661 cggtgcctga tgcgacgctg gcgcgtctta tcagcatacg ccacatccgg cataccatgc
      721 cggatgtggc acatcattac aacgcaatat ccgccacttc tttttgaacg ggttgcggtt
      781 tcaactgcgg ttttggcgta ctatctgctg cctgcggctt gtcgtatttc agccccagca
      841 cttcgctggt gtactgcaac tcgcgttctg ttgccgctac atcaccgttc agcttgcgtc
      901 cataagacgg aacgatcgct ttcaacgtag cctgccattg cgggctggaa acgcgatcgc
      961 caaatacttt ttccagcaga tccaacataa tcggcgcggc ggttgacgcc cctggcgatg
     1021 cccccagcag cgcggcaatg gtcccttgct ggtcactgac cacttcagta cccagacgca
     1081 gtacaccacc tttatcggca tcacgcttga taatctgcac gcgctgcccc gcttgccaca
     1141 aacgccagtc ctcttttttc gcttgcggat agtactcttt caacgcttca aaacgatcct
     1201 cttcactcaa catcacctga ctcaccagat atttcaccag atcgaaatta tccagcccga
     1261 cgtgcatcat cggcatcacg ttagaggtgg tggtggaact catcagatcc cacaatgaac
     1321 cgtttttgag gaatttggtt gagaaggtgg caaatggccc aaacagcact acgcgtttac
     1381 cgtccagaac gcgggtatcg atatgcggaa ccgacatcgg tggtgcgcca acggatgctt
     1441 taccgtaaac cttcgccaga tggtgattaa ccacgtccgg gttttccgaa acaaggaact
     1501 gtccgcccac cgggaaaccg gcgtagtctt tcgcttccgg aatccccgat tcctgtaata
     1561 gcttcagcgc cgcaccgccc gcgccgataa agacaaattt ggcacgaatg ttctgtgcag
     1621 tgccattttt tagatcggca acggtaacgg tccaggtgtt atcgtcatta cgctttaggg
     1681 cgcggacttc gctgctgagt tgcagcgaga agttagattt cttctgcaag gaagcaatta
     1741 actggcgggt gatctcgccg tagttcacat cggtaccaat ttccgtacgc gtggctgcca
     1801 ctttctgttg cggatcgcgc ccttccatca ctaaaggtgc ccactctttg atctgcgcgt
     1861 gatcttcaga gtaacgcata ccgcgaaaca gcgagctttg ttgcaacgcg gcgtaacggg
     1921 cgcgcaggaa attaacgtta tcctcgcccc agacaaagct catatgcgga acggtattga
     1981 taaatgaacg cggagtacgc agcacgccgc gctcgacctg gtgcgcccag aactggcggg
     2041 aaatctgaaa tgcttcgtta atggcgactg ctttttcaat actgatgctg ccatcggcgt
     2101 tttgcggggt gtagttcagt tccatcagtg cagaatgccc ggttccggcg ttattccagc
     2161 cgttcgaact ctcctgcgcg acaccctcca ggcgctccac catggtcatc gaccattcag
     2221 gctccagctc gcgtaaatag gtccccaacg tggcgctcat aatgccgccg ccaatcaaca
     2281 gtacatcagt ttcctgctcc tcggacgctt tcgcttttgc cgccatcgaa acggcattaa
     2341 gccccacggc catcgagaag agcatggcag tcactttttt catcttgtta atgccttact
     2401 tttagtcgct ttattgcagg tgagatttgc gcggcatcaa cggtaaca
//
LOCUS       DAZFXT010000274         2435 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.277, whole genome shotgun sequence.
ACCESSION   DAZFXT010000274 DAZFXT010000000
VERSION     DAZFXT010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.277"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..578
                     /locus_tag="ACHKW0_005067"
     CDS             <1..578
                     /locus_tag="ACHKW0_005067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000957425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HFO7477709.1"
                     /translation="NEESRAAPEFGYHFHVPPEYFSLSTNDYNFTYDIYQAGLTIYRM
                     CVGHDNFERERSAFSTIEQLRESIINGCYPLKEYPPHIHKKLITIVNKCIHVDPNERY
                     QSVLDVLNDLSAISDGVLDWRLQMTKPTNGTCEWQKKSGDAILSIVFDAENSSTTGFR
                     LYDDGRKRRATNLTISSGCTPTKLYRLLKDN"
     gene            581..745
                     /locus_tag="ACHKW0_005068"
     CDS             581..745
                     /locus_tag="ACHKW0_005068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477710.1"
                     /translation="MKKREEVSKLPRRRDAALAVPYKKDEFISPSDDKKFSKAKSFTS
                     TSLKDKYFKI"
     gene            1559..1627
                     /locus_tag="ACHKW0_005069"
                     /pseudo
     CDS             1559..1627
                     /locus_tag="ACHKW0_005069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311021.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
     gene            1689..2312
                     /locus_tag="ACHKW0_005070"
     CDS             1689..2312
                     /locus_tag="ACHKW0_005070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nmad5 family putative nucleotide modification
                     protein"
                     /protein_id="HFO7477711.1"
                     /translation="MSSIRLTTRMKEEIARNALIKSGVFTELEEVTKLKNQLALDARV
                     IAFGGKKKTEEVDRLSSKLVAISEELGKMGCSFYSYDVSSTSIYLTVSGRRVGWHSYG
                     KDGNGKDILLPTPIKDKCMFDAEHEITKRFDEICALQQKLEAKKKDIESNVWAALNSV
                     TTVKRLIEVWPESKELLPKEADKASTALPALRVEDLNKMIGLPSEAA"
BASE COUNT          821 a          487 c          519 g          608 t
ORIGIN      
        1 tcaatgagga atcgagagct gcgcctgagt ttggatatca ttttcatgtg ccaccggaat
       61 atttttcttt atcaacaaat gattataatt tcacatatga catatatcag gcaggattaa
      121 ccatatatag aatgtgtgtt ggacatgata attttgaaag agaaagatct gcatttagca
      181 cgattgaaca actcagagag tcgataatta atggctgcta tccattaaaa gagtatcctc
      241 cccatataca taaaaaatta ataacaatag tgaacaaatg cattcatgta gatccaaatg
      301 aaagatatca atccgtacta gatgtactaa acgatctctc agctataagt gatggcgttc
      361 ttgactggcg tctacagatg acgaaaccaa ctaacggcac atgcgaatgg caaaaaaagt
      421 ctggggacgc tatactgtct atagtttttg acgcagaaaa ttcgtctact actggttttc
      481 gtttatacga tgatgggcgg aaaaggcgtg ctacgaactt aacaatatcc tcaggatgta
      541 cccctacaaa actgtatagg ttattaaagg ataactgatc atgaaaaagc gcgaggaagt
      601 aagcaagctg cctcgcagac gtgatgcagc attagcggtt ccctacaaaa aagatgagtt
      661 cataagccct tctgatgaca aaaaattttc aaaggcgaaa agttttacat ctacatctct
      721 aaaagataaa tactttaaaa tctagcccgg cctcagcgcc gggttttctt tgcctcacga
      781 tccccacacc taaaaacaca taaccaattg tatttgttga aaaataaata gatacaactt
      841 gctaaacaac gcaatccaga tctccctcaa atctctttat ttatcctgtc gaattcctac
      901 aacaaaataa aacaccataa gaatcaatac gatatttgaa aaccaagaga atttatcatt
      961 ttgctattgc cattaattta tcattccgat aaagttcacc catcagcagg acgcactact
     1021 caccagggcg gtgaatatac aacgattcga atatgaatct acggcgctga caaagcgcaa
     1081 taaccaaagt gaactttggg gtgtggtgaa gggttcatgg acgggaatat gtcgcacgta
     1141 aagcggcgag gcctgcggga ctattgccga attgaagtag gccgaaacag gtcgaaatgg
     1201 gtctcccacc taccacacca ccaaagttca tcaggaggtc tatatgacac gcagaactca
     1261 gttcaaaggc aattcacgtt ctcgtcgtcg tgagcgttta aaggcaaagg cattagctaa
     1321 cggcgtgctg gcccgcgaag aagcaataag ttcagaagta ttacaccgcc ctactctaag
     1381 cagagcgcag attcaggcta aaggtactca cgaaacgcct gagcgcatag aagacgctaa
     1441 gccaattaag ttcatggcac aggacgtgat ctggcaacag aaagaataca gacgcaatct
     1501 ggagcgagcg gccattgtgt acgcgaatga gtttggacat aagcaaccag aaactggtgt
     1561 atgtcttcca aacgtagctc tttacgcggc aggctaccgg aaatcaaaac aactgacggc
     1621 aagatgactt gtgttggtcg ccagaaaatg aaattaggca gcaaaccact tatttgagag
     1681 gaattaatat gtcatcaatc cgcttaacta cgagaatgaa agaggaaatc gctcgtaacg
     1741 ctttaattaa gtccggggtg ttcactgaac ttgaagaagt aacaaagtta aagaaccagc
     1801 ttgcacttga cgccagagtt attgcgtttg gcggtaaaaa gaaaactgag gaagtggatc
     1861 ggttatcatc caagttggta gctataagtg aagaacttgg aaagatggga tgttcatttt
     1921 actcatacga tgttagttct acttcaattt atctgactgt atctggcaga agggttggat
     1981 ggcattcata tgggaaagac ggcaacggca aagatatatt gctccctact ccgatcaaag
     2041 ataaatgcat gtttgacgca gaacacgaaa taacaaaaag gtttgatgaa atctgcgcat
     2101 tgcaacaaaa acttgaagcc aagaaaaagg atatcgaatc aaatgtatgg gctgctttga
     2161 actcagtcac aacagttaag cgacttattg aagtttggcc tgaaagcaaa gaattgctac
     2221 caaaagaagc agataaagca agtacagcac ttcctgcttt acgggtagaa gatttgaata
     2281 agatgattgg acttccttcc gaggccgcat agtcggcctt tatttttggc ataaacaaca
     2341 gaataaatac tgcactgtgt attcattcca acgagtgaat acacggagca atgtcgctcg
     2401 taactaaaca ggagccgact tgttctgatt attgg
//
LOCUS       DAZFXT010000275         2421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.278, whole genome shotgun sequence.
ACCESSION   DAZFXT010000275 DAZFXT010000000
VERSION     DAZFXT010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.278"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            490..715
                     /gene="ykgL"
                     /locus_tag="ACHKW0_005071"
                     /pseudo
     CDS             490..715
                     /gene="ykgL"
                     /locus_tag="ACHKW0_005071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YkgL"
     gene            complement(750..890)
                     /gene="ykgO"
                     /locus_tag="ACHKW0_005072"
     CDS             complement(750..890)
                     /gene="ykgO"
                     /locus_tag="ACHKW0_005072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HFO7477712.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(890..1153)
                     /gene="ykgM"
                     /locus_tag="ACHKW0_005073"
     CDS             complement(890..1153)
                     /gene="ykgM"
                     /locus_tag="ACHKW0_005073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HFO7477713.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGA"
     gene            complement(1517..1618)
                     /gene="ykgR"
                     /locus_tag="ACHKW0_005074"
     CDS             complement(1517..1618)
                     /gene="ykgR"
                     /locus_tag="ACHKW0_005074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YkgR"
                     /protein_id="HFO7477714.1"
                     /translation="MKENKVQQISHKLINIVVFVAIVEYAYLFLHFY"
     gene            complement(2092..2181)
                     /locus_tag="ACHKW0_005075"
                     /pseudo
     CDS             complement(2092..2181)
                     /locus_tag="ACHKW0_005075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308358.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          710 a          460 c          527 g          724 t
ORIGIN      
        1 cttgcatttt atattagcca ggaactttgc cctgaggtgt taggaatagt cttaatgtgt
       61 tgtgtaatgg actagtaaaa aaacaagttt aaatgataag aaatagaata taaaactatc
      121 atgagtcata attaaagatg atttgccaat cagtaaggtg ttttaatgga ttaacatgcc
      181 gcacaaaatg gttatgggtg ttcatcccaa taagtgatgc aaaattaaac aggtataagt
      241 acgtcatctg tccggtaagc cccctaagag ccaaattaag tgaagttaaa cgaagactgc
      301 cagttgtcac aggaacgatg gcctctttgc cagggcactg cattcagtca tctgtgcgcg
      361 ttactctaac tggcactcga acgtcggcga tggggttacc ggtggatcag gccgttcctg
      421 tggcgtaaac gtttttatgt cagtaccaac agatttacca catttatcat cttaacagtt
      481 tgagtgtaaa tgcaggctgc gtcgcaaagg gaggaaactg agtggcgtgt tcagtctaaa
      541 agagggttga tgccagcata cagggcgaag ctggtcaaca agtcaacatt aatataatgg
      601 aatattcgga gagaaacgtc aggcagttga ccttaaatga acaggaagat acatccccag
      661 aaaaattaat gctggtgtaa taaatacacc aacattaatc cggtctgatt attaatcaga
      721 caaatatcgt taattcgtcg aattttaaat caacgttttt tcttacgacc ctgaacggcc
      781 ttaaaacgtg gattagattt acaaatcaca tacagccgtc cttttcgctt cacaatctga
      841 cagtctggat ggcgttcttt tgcggtacgc agagagttaa ggactttcat catgccccct
      901 ttttcgtgct aacaaaacga ccaaaacgtt gggtgaaccg tgcaacattt ccttctgatg
      961 ccactgttct cagcttccct gtatagaacg ggtgcgattt cgaagagaca tcaattgtca
     1021 cgtatggata cgttacgcca tccagctcaa tctcacggtc tgttttgata gtcgagccga
     1081 ttttaaagta ctcatcaaca ctggtgtcgt ggaacaccac agtacgatac tcaggatgga
     1141 tattgggctt catcattttt acctgttatg ttataacata accataaaga tacatgctca
     1201 tccggatcat ttcaacaccc aatccctgtg cccatttttg gtatgtggaa tttccccaac
     1261 ggggaagtct ggcattgtta gcagccgttg gcgacgctcc cataaacgcc tggatgagtg
     1321 attgatgatg gtttcgctga gagtatggat ttttcgacaa tggaggttat ctccacttta
     1381 ctggccgatg atttatcgca gtatgattga ttacctgaca gcgtgtattc ctctcccacg
     1441 gatataataa ttacttacaa aaagggagag gatgcatatt ttaaatatca ctgaagtgaa
     1501 cagtttattg ccgttattaa tagaaatgga gaaataaata ggcgtattct acaattgcga
     1561 caaaaacaac gatattaatc agtttatgac tgatttgttg tactttattc tctttcattg
     1621 gtacttcctc gctttaaaaa agagcgcact tcgtaagtgc ccttatataa ataacgagtt
     1681 tggtcaacca attttttgac atgtatcaca aatttgaatg gatgtattac atcggctatc
     1741 ttttattgca ccaacgtcat tgatatatgt cgcctgaagt cagttccggg aatgagtctg
     1801 atctcaagac tggcccagtc cgggcgttga ttggcgctga ggagcatatc gcatctcatc
     1861 ataatgtcgt atctcctggg gtgttataca agatatcgtt gttggtgacc tgggagagga
     1921 attgagttct attaaaccgt caactatgcc ggatacatac tggattacac tgcaggcacg
     1981 ccttacgaga gaacgtgccg cagtgacggg ttaattatct gaaagaattt gcgaggctgt
     2041 atcggttact cattggtttg atagttttac tctcgggaga gtaatagata tttaattcat
     2101 taacggaaac cagtcagttc ctttcgatgc ctgaatttga tcccatagtt tatccggaat
     2161 ggttaattca tgaacaagtt gtgttatcgt tcatgagaag cataacgtaa agggaaaagc
     2221 tcgattagac ggcaggattt gtcaggggtt atgaacgaaa ttcataaatc tgtttgagtg
     2281 ttgcgatggg tagtgcaagt tcgatatctc cgcaatttac agtctgatga atgaaaatga
     2341 atatccataa aaaatatatt ggtttatcct ggcgtatata cctacttcga tgtattgcca
     2401 atcgtggtaa attaaagaca g
//
LOCUS       DAZFXT010000276         2387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.279, whole genome shotgun sequence.
ACCESSION   DAZFXT010000276 DAZFXT010000000
VERSION     DAZFXT010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.279"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..276
                     /locus_tag="ACHKW0_005076"
     CDS             <1..276
                     /locus_tag="ACHKW0_005076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7477715.1"
                     /translation="VNLMHHRKAGNTKLGEFGDYGNDWQTLELVFTAGGATVTPKLNG
                     VAGPAFQVIKDSLTLGLNALTLTDVTKNAAYGVEIESLMLEINAPAA"
     gene            568..783
                     /locus_tag="ACHKW0_005077"
     CDS             568..783
                     /locus_tag="ACHKW0_005077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0020002 - host cell plasma membrane
                     [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HFO7477716.1"
                     /translation="MHQMEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            788..1132
                     /locus_tag="ACHKW0_005078"
     CDS             788..1132
                     /locus_tag="ACHKW0_005078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HFO7477717.1"
                     /translation="MKKKYELGVKGINNYPDKITVTVALEIGGYPSLLLPDVAISLDR
                     TEGATLEFYEAEAKKQAKQFFMDVAAGLCEGDGPLPEKRPVILEAQDVLITYRGKLPG
                     IITGSLKTPPLA"
     gene            1183..1716
                     /locus_tag="ACHKW0_005079"
     CDS             1183..1716
                     /locus_tag="ACHKW0_005079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7477718.1"
                     /translation="MNAKIRYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGTTRVDGKPVVPGMKLSKEKCDQVNAIERDKALEWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDLKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     VSRRDQESALACWGIDR"
     gene            1872..2054
                     /locus_tag="ACHKW0_005080"
     CDS             1872..2054
                     /locus_tag="ACHKW0_005080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310767.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477719.1"
                     /translation="MGKYKRILLRLTMKNGLELKAPVTDDVSRALAFAIKWVAVGIAV
                     SPMLYGLAKLVIALKS"
     gene            2067..2198
                     /locus_tag="ACHKW0_005081"
     CDS             2067..2198
                     /locus_tag="ACHKW0_005081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477720.1"
                     /translation="MSDKLITLAKILCVIVGISFSLMLVAICFSTAWRVLTLSWLVG"
     gene            2206..>2387
                     /locus_tag="ACHKW0_005082"
     CDS             2206..>2387
                     /locus_tag="ACHKW0_005082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7477721.1"
                     /translation="MNRVLCVVIIVLLVACGVLSLGLNHYRDNAITYKAQRDKKASEL
                     KLANVTITDMQVRQRDV"
BASE COUNT          611 a          490 c          699 g          587 t
ORIGIN      
        1 gtgaatctga tgcatcacag gaaagccgga aacacgaagc tgggggagtt cggcgattac
       61 ggtaacgact ggcagacgct ggagctggtg ttcaccgccg gcggtgccac ggttactccg
      121 aaactgaatg gagtggctgg cccggcattc caggttataa aagacagtct gacactggga
      181 ctgaatgcgc tgacgctgac ggatgttaca aaaaatgcag cgtatggcgt tgagatagaa
      241 agtctgatgc tggagataaa tgcaccggca gcataataaa aaaacagcca gtactgactt
      301 tcgtcggaga agtactggct aagaaggata gttgggtttc acatgatact tatatctggc
      361 agtacatttt ctgacagaca gtgatgggtg ttgtcaagat attgtgtcat ttataacctg
      421 aatcaggggg ggagccggaa tgttatctgg catttttagc agagcctgaa tgccataatc
      481 acggctcccg gcgttggccg tcagtgggcg acactggcgg ctttttgttt tcctttactt
      541 tcattttctg tcggcggtga cggagacata catcagatgg aaaaaatcac aacaggtgtg
      601 tcatacacca cgtcagcggt ggggacggga tactggttac tgcagctgct ggacaaagtc
      661 tctccgtccc agtgggtggc aatcggtgtg ctggggagtc tgctgtttgg cctgctgacg
      721 tatctgacta acctgtattt caaaatcaga gaggaccgtc gtaaggcggc gcggggggag
      781 taaagcgatg aagaaaaaat acgaactggg tgttaaaggg ataaataatt acccggataa
      841 gattactgtt actgtggcac tggaaattgg tgggtatccg tcactgttgt tgccagatgt
      901 ggcgattagt cttgaccgta ctgaaggtgc cacgctggag ttttacgaag ctgaggcgaa
      961 aaagcaggcg aagcagtttt tcatggatgt tgctgccggg ttatgtgaag gggatggtcc
     1021 gttgccggaa aagcgccccg taattttaga ggcgcaggat gtgttgataa cctacagagg
     1081 aaaactaccg ggaataatta ctggttctct gaagactcca ccgctggcct gaagacttaa
     1141 catatccagg gatttgaaat cgataaaccc tgataaatat ccatgaacgc aaaaatcaga
     1201 tacggcctgt cggctgccgt tctggcgctg attggtgcag gggcgtctgc gcctgaaatc
     1261 ctcgaccagt ttcttgacga aaaagaaggt aaccacacca cggcataccg tgatggtgcg
     1321 gggatctgga ccatctgccg cggtaccacc cgagtggatg gtaaacctgt cgtcccgggc
     1381 atgaagttgt cgaaggaaaa atgcgaccag gttaacgcca ttgaacgtga taaggcgctg
     1441 gagtgggtgg agcgcaatat taaagtaccg ctgaccgaac cccagaaggc gggtatcgcg
     1501 tcattttgtc cttataacat tggccccggt aagtgtttcc cgtctacgtt ttacagacgg
     1561 attaatgcag gtgatctaaa aggtgcctgc gaagcgattc gctggtggat taaggacggt
     1621 ggcagagact gccgtattcg ctcaaacaac tgctacggtc aggtttcacg gcgtgaccag
     1681 gagagcgcgc tggcgtgctg gggaattgac agataagcag aatattttgc tgaaaaatgc
     1741 ggtttgctca cactggcgga taacacgaaa tcctgcgaac tggcaaaaac taagtgaata
     1801 aaagtaaaac cccgtttgtt ggccgcaaac ggggttttgt gtttctgacc ttgagtaagg
     1861 caagggagaa catgggaaag tataaacgaa ttctgttgag attgactatg aaaaacggcc
     1921 ttgaactgaa agcgcctgta actgatgacg tcagcagagc gctggctttt gctattaagt
     1981 gggtggcggt cggtattgct gtgtctccga tgctgtatgg gctggcaaaa ctggtcattg
     2041 cgttgaaatc gtgaagagga ttaagcatgt cagacaagct cataacgctg gcgaagatcc
     2101 tctgtgtaat cgtcggcatt tcattttcac taatgctggt tgctatttgc ttttccactg
     2161 cctggcgcgt cttaactttg tcatggctgg tggggtgagg gggatatgaa ccgtgttctg
     2221 tgtgtggtga ttattgtcct gctggtagcc tgtggtgtgc ttagtctggg gctgaatcat
     2281 taccgcgata acgccattac ctacaaagcg caacgcgata aaaaagccag tgagctgaag
     2341 ctggcgaacg tgacaattac tgatatgcag gtacgccagc gtgatgt
//
LOCUS       DAZFXT010000277         2382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.280, whole genome shotgun sequence.
ACCESSION   DAZFXT010000277 DAZFXT010000000
VERSION     DAZFXT010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.280"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..389
                     /locus_tag="ACHKW0_005083"
     CDS             <1..389
                     /locus_tag="ACHKW0_005083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477722.1"
                     /translation="RLSPHRLIKVLELFNASQSKALPFAEHSKYIYVMHKNQRKYLSI
                     FEGDKTLPVVDYMVVLNHGTTVDGEEKNIISAVFLSNMDGNVQNVPVRLKLKSQLRTG
                     YAERYQAIASQYSSRIGVDYVSMMLP"
     gene            complement(461..877)
                     /locus_tag="ACHKW0_005084"
     CDS             complement(461..877)
                     /locus_tag="ACHKW0_005084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477723.1"
                     /translation="METLFKVFEKFSSRPLFFIFFGLSLCEFFQKQSVLMNPSADNIA
                     KLFAAMILVVFFTWGFEWLIFKFNVNLEPHDQGDIGPTIGTATLAVYLVYAFHFLSEN
                     PEALNLKLLTNSGFIYSTTLLLFSLECMKLRRLKQK"
     gene            complement(916..2145)
                     /locus_tag="ACHKW0_005085"
     CDS             complement(916..2145)
                     /locus_tag="ACHKW0_005085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase domain-containing protein"
                     /protein_id="HFO7477724.1"
                     /translation="MAKIAKKLTDTEIKSTKPADKEINLFDSDGLILRIAPLAKGGKK
                     NWYFRYAVPVSKKRTKMSLGTYPHLTLARARALRDEYLSFLANGVDPQIHNNDKAKAL
                     KSATEHTLQAVARKWLDEKVKTSGISQDHAADIWRSLERNVFPGLGNVPINEIRPKLL
                     KQHLDPIEQRGVLETLRRIISRLNEIFRWAATEELIEFNPADNLGQRFSKPKKQNMPA
                     LPPSELPRFMESLTNASIRLETRMLIEWQLLTWVRPGEAVRARWSDIDTTNSIWNIPA
                     DFMKMKKLHKVPLSKEALRILELMKSISGHREWVFPSIKAPLNHMHEQTANAAIIRMG
                     FGGELVAHGMRSIARTAAEESGKFRAEVLEAALAHSKKDEIIAAYNRAEYLIERQSLM
                     QWWSDYVQAQRSNALVA"
BASE COUNT          653 a          466 c          490 g          773 t
ORIGIN      
        1 taagattaag tccgcacagg ctcattaaag ttctggagct ttttaacgcc agtcagagta
       61 aagcattgcc atttgctgaa cattcgaaat atatatatgt aatgcataaa aatcaaagaa
      121 aatatctctc tattttcgaa ggggataaaa cgcttcctgt tgttgattat atggtggtgt
      181 tgaatcatgg aacaacagtt gatggcgaag aaaaaaatat tatttctgcc gtgtttttaa
      241 gtaatatgga tggcaacgtg caaaatgttc ctgtccgact caaacttaaa tctcaactga
      301 gaactggtta tgcagaaaga tatcaggcta tagcgtctca gtatagctca aggattggtg
      361 tggattatgt atcaatgatg ctaccataat tattatattt taggcgtggt gatttttttt
      421 cgccatgcct attttatatt tatcatcaca atgatgttgt ttatttttgt ttaagtcttc
      481 taagcttcat gcattctaat gagaataata atagagttgt gctgtatata aagccagagt
      541 tagttaataa ctttaaattt aatgcttcag gattttcact gagaaagtga aaggcataaa
      601 ctaagtatac agctaaagta gccgttccaa ttgttggtcc aatatcgcct tgatcatgag
      661 gttcaaggtt tacattgaac ttgaagatta gccattcaaa tccccaagta aaaaaaacaa
      721 ctaatatcat ggctgcgaat aatttcgcga tgttatctgc tgatggattc atcagaacag
      781 attgtttctg aaaaaattca caaagtgaga gtccgaaaaa aataaaaaaa agtggtctgg
      841 aactaaattt ttcaaaaact ttgaataggg tttccatttt ctttaccttc aggattatat
      901 tagctattct gatacttagg ctaccagagc atttgatctt tgagcttgaa cgtaatcact
      961 ccaccattgc atcaaactct gtcgctctat cagatattct gcacgattgt atgctgcgat
     1021 aatttcatct tttttcgagt gggcaagcgc tgcctcaaga acttcagctc tgaatttacc
     1081 agactcctct gccgctgttc gtgcaataga acgcataccg tgagctacaa gctcgcctcc
     1141 gaaccccatt cggatgatag ctgcgttggc tgtttgttca tgcatatgat taagaggcgc
     1201 ttttatgctg gggaaaaccc attctctatg cccacttatt gatttcatta attcaaggat
     1261 gcgcaaagct tctttactca aaggaacttt gtgaagcttt ttcattttca tgaaatcagc
     1321 aggaatgttc caaatgctgt tggttgtatc aatatcagac caccttgcgc gaacggcttc
     1381 acccggacga acccatgtca acaattgcca ttcaattagc atacgtgttt ccaaccggat
     1441 tgacgcattc gtcaaagatt ccataaacct tggcaattcg cttgggggaa gggcaggcat
     1501 attttgcttt tttggtttac tgaatctttg accaaggttg tcagccgggt tgaactcaat
     1561 aagttcttca gtagctgccc accggaagat ttcattcaga cgtgaaatga tacggcgtag
     1621 agtttccaat acgcctcgtt gctcaatagg atcaaggtgt tgttttaaga gcttaggtcg
     1681 gatctcattg atagggacat tacccagacc gggaaagaca tttctctcta agctgcgcca
     1741 gatgtctgct gcatggtctt gtgagatacc tgatgtcttt accttctcat ctaaccattt
     1801 ccgcgctacg gcttggagag tgtgctcagt agcactcttt aatgccttcg ccttatcgtt
     1861 gttatggatt tggggatcaa caccatttgc cagaaaggag agatattcat cacgtaaggc
     1921 tctggctctt gcaagggtaa ggtgaggata tgtcccaagg ctcattttgg ttcttttctt
     1981 gctcactggt actgcatacc tgaaatacca atttttcttg cctcctttcg ccaaaggagc
     2041 gattcgtaga atcagaccat cactgtcaaa caagttgatt tctttatcgg ctggcttggt
     2101 gcttttgatt tcagtgtcag tgagcttctt agcgattttt gccattttgg gaccctcggt
     2161 ttttggaccc ttcttagtgg gtcccattca gggtgccata actcgtagtt ctcagcaatt
     2221 ctcactggac gacaatagac gtaaaaaagc ccgcagagct tgtgctgtgt gggcttagta
     2281 gacttcattg aacttcaaac aactaaaaag tggtggagct ggcgggagtt gaaccagcgt
     2341 ccatttgttt ttaatttatt gatttttatt gttttatttt at
//
LOCUS       DAZFXT010000278         2361 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.281, whole genome shotgun sequence.
ACCESSION   DAZFXT010000278 DAZFXT010000000
VERSION     DAZFXT010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.281"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..195)
                     /locus_tag="ACHKW0_005086"
     CDS             complement(<1..195)
                     /locus_tag="ACHKW0_005086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477725.1"
                     /translation="MLKQQDMTETARVVFDELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRLQS"
     gene            complement(482..1300)
                     /locus_tag="ACHKW0_005087"
     CDS             complement(482..1300)
                     /locus_tag="ACHKW0_005087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysostaphin resistance A-like protein"
                     /protein_id="HFO7477726.1"
                     /translation="MWIVLVLSLSTLSWHKVVAFSLLTVSVVLAVLNDIIDWSVLFFV
                     ATIVFFIILKFNWKYNAWAKSIYEVGIVLSAIALSFHLWPGFHNPVVLNSVTVGPQST
                     PYTMYFNFDKALVPFLLVLCTSSLFKKEVKSEVSLWKWGALSLSVPLILFLAVFFGGL
                     KPEIHFPEWLPEFILANLFFVSLAEESLFRGYIQSRLSEVTSPLVALIVAALLFGFYH
                     YSGGALLVLFATLSGVVYGLSWMWSGRLWVATLFHFGLNLCHLLFFTYPFLKHN"
     gene            complement(1452..1823)
                     /locus_tag="ACHKW0_005088"
     CDS             complement(1452..1823)
                     /locus_tag="ACHKW0_005088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7477727.1"
                     /translation="MARDIQMVLERWGAWAANNHEDVTWPSIAAGFKGLIPTKVKSRP
                     QCSDDDAMIICGCMARLNKNNQYLHDLLVDYYVGGMTFMALARKHRCSDGLIGKRLYK
                     AEGIIEGMLMALNVRLDMDMR"
     gene            complement(1813..2184)
                     /locus_tag="ACHKW0_005089"
     CDS             complement(1813..2184)
                     /locus_tag="ACHKW0_005089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7477728.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAEVLIDDEQFDEINIVRGQ
                     PVPGGRLGVKIYEIRGGNDGA"
     gene            complement(2197..>2361)
                     /locus_tag="ACHKW0_005090"
     CDS             complement(2197..>2361)
                     /locus_tag="ACHKW0_005090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7477729.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT          780 a          596 c          410 g          575 t
ORIGIN      
        1 ggactgaagg cgtctgtaac aaccgcactg atagtctgcc agacccgcca taacaagctg
       61 ggtcagtatt aactggcagc gttcgcgtga aaggtaagta ttctgcgcaa tctccccgac
      121 tgtcgccggt tcggtgacgc ttaattcatc aaacacaact ctggcggttt ctgtcatatc
      181 ctgctgtttc agcatgtctt tttacccttt ccggttaacg tgacacacca ataactcttg
      241 ccgaaaaagc cagcaagctg aaagacctgt attaataact accagcacat ttaacgcact
      301 gcgctacttt gcgggcacaa aaaacccgct cagaggcggg gtcaagctat gcggcgaaat
      361 aaccactctt aacagcatac ctgatttttt acgtacgtaa atgctttgcc gtgcatattt
      421 ttcatgcaaa tgtcacgtcc tgctattttt cagtcttata aatttaaaac catagaaaaa
      481 atcaattatg ttttaaaaat ggataggtaa agaataacaa gtgacacaga ttcaaaccaa
      541 aatggaaaag ggtggcaacc cacaaacgcc cactccacat ccatgacaat ccatacacaa
      601 caccagataa cgtggcaaat aatacaagta aagcaccacc tgaatagtga taaaaaccaa
      661 acaacaaagc cgccacaatt aatgcaacca atggagacgt tacttctgat agccgtgatt
      721 gaatataccc tctaaataat gattcctctg ccagagacac aaaaaacaaa ttagccaata
      781 taaactctgg caaccactca ggaaaatgaa tctctggctt taatccacca aaaaaaacag
      841 ccaaaaacag gataagagga acagagagcg acagagcccc ccacttccac aaagacactt
      901 ctgattttac ttctttttta aacaaagaag atgtacacag gactaacaaa aatggcacca
      961 gcgctttatc aaaattaaaa tacattgtat agggagtact ctgagggcca acagtaacag
     1021 aatttagcac tacaggattg tgaaaccctg gccatagatg gaaagataat gctatggctg
     1081 ataaaactat gccaacttca tatatagatt tagcccaggc gttatatttc cagttgaact
     1141 tcaaaataat aaaaaaaacg attgtagcaa caaaaaataa caccgaccaa tcaataatat
     1201 cattaagcac agccaggaca acagacaccg tcaacaatga aaaagccact accttatgcc
     1261 aactgagagt tgacagtgac agtactaaca caatccacat aagcactcct tttatttaat
     1321 gatgaagatt gattatcaat attttcaatt caccaggcaa cattttatct accttccaca
     1381 atacatgacc atcaaaaaat aaacatgtta attataaaca cagaaaacat aaccctcatc
     1441 actatatatc cctaccgcat atccatatct aaccggacat tcagagccat aagcattcct
     1501 tcaataatac cttccgcttt ataaagcctt ttaccaataa gcccatcaga acatctatgc
     1561 ttacgtgcaa gagccataaa tgtcattcca cctacgtaat aatccaccaa caaatcgtgc
     1621 aaatactgat tattcttgtt taatcgtgcc atacaaccac aaattatcat ggcgtcatca
     1681 tcagaacact gaggacgtga tttcacttta gtcgggatta atcctttaaa accagcagct
     1741 atcgagggcc atgttacatc ttcatgatta tttgctgccc atgcccccca tcgctcaaga
     1801 accatctgga tatcacgcgc catcgttacc acctctgatt tcgtaaatct tcacgcccag
     1861 ccgtccacct ggcacaggct gaccgcgcac aatattgatt tcatcaaact gctcgtcatc
     1921 aatgagcact tccgcatgcg tcagcgcatc cagcggtgct ttcagaatgt tgtccaggtc
     1981 acggcggcgc ttatccggtg gttctgcaat aatttttatt gccagccgtc cggacaggct
     2041 taatttcagc cgctgctggc gaacaataag cgccacagcc cggcgataac gctcaccggc
     2101 ttttgataca aaatatgtgc tgccacgacg tcgccagtag gtgttcaccg tcggcgggta
     2161 aggcaaaaca aactctatac gcatcagtaa cctcttttac ccgagcacgc cggttgcaaa
     2221 agcgtgatca agaaaacgaa aaattaaatc aacctgggaa ccatgctttt cttcgaacgc
     2281 cagcggatcc gcatgaagct cgttgtgatg ctcccgacac agcggtagcg tgaaaatatc
     2341 gtgggatttt gttcccattc c
//
LOCUS       DAZFXT010000279         2345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.282, whole genome shotgun sequence.
ACCESSION   DAZFXT010000279 DAZFXT010000000
VERSION     DAZFXT010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.282"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..348)
                     /locus_tag="ACHKW0_005091"
     CDS             complement(<1..348)
                     /locus_tag="ACHKW0_005091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7477730.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLDTDARKTASAV
                     EQGLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAG"
     gene            complement(329..742)
                     /locus_tag="ACHKW0_005092"
     CDS             complement(329..742)
                     /locus_tag="ACHKW0_005092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7477731.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRMHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLVPRHEEEQVERPDEDKMLMQKAAGLAGGVRFGGDGGRD
                     ILSSADVADVMVDDAALMMASAGISGGVRYVPAGW"
     gene            complement(769..1200)
                     /locus_tag="ACHKW0_005093"
     CDS             complement(769..1200)
                     /locus_tag="ACHKW0_005093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7477732.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTGSPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGPVHVDTDITEVAKNNALTDDDFSAGKSSTAS"
     gene            complement(1214..1966)
                     /locus_tag="ACHKW0_005094"
     CDS             complement(1214..1966)
                     /locus_tag="ACHKW0_005094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477733.1"
                     /translation="MPTPNPLAPVKGAGTTLWVYTGKGDAYANPLSDDDWQRLAKVKD
                     LTPGEMTAESYDDNYLDDEDADWSATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSEITP
                     ATAIKVTPTSGTVEKGKTTTLTVSFEPESATDKTFRAVSADPSTGTIAVKDMVITVTG
                     VKAGKVSIPVISGNGQFATVAEVTVTEAGAAG"
     gene            complement(1974..>2345)
                     /locus_tag="ACHKW0_005095"
     CDS             complement(1974..>2345)
                     /locus_tag="ACHKW0_005095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7477734.1"
                     /translation="AVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLTDAEYTGEE
                     LDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMSAIPALAGMITTMVQQGYDYR
                     RDDDMALWSSADLTYSITYEM"
BASE COUNT          529 a          741 c          548 g          527 t
ORIGIN      
        1 accggcaagc cccctgaaca tggggatcat cccgccgaag gagtccttca cctgaccgcc
       61 ctgttgcagc aggatcagcc agggattctg accaccggca agctgcgtgg cgatatccgt
      121 aaactgtgcg ggcagggttc gcatggccgc tttatactgc ccgacggaaa tcccggcttt
      181 ttgtgcagcc agcgcctggc ggctcaggcc ctgttcaaca gcactggcgg tttttctggc
      241 gtcggtatcc agacctgaaa aatgacgcct tacccggctc atctgctcat cgaaacggac
      301 cgcatccaga ctaaggtcaa taacaagatc accaaccggc tgggacatat ctcacacctc
      361 cggaaatccc cgctgaagcc atcattaatg cggcatcatc caccatgaca tccgccacat
      421 ccgcagacga taaaatatcg cgccctccgt ccccaccgaa ccggacgcct ccggcaagtc
      481 ctgccgcttt ctgcatcagc attttgtcct catccggcct ctccacctgc tcttcctcat
      541 gccgggggac aagcagactg aaatcagagg gatgcatatc cggatcgcaa aaaaacaggc
      601 tgagtacagc gtacgtcagc ccggaaaaat gcatatccag ctgggtatcg tgaaaataat
      661 gcatgcggta aaaatgtcgc cagtcggcat attcggtgga tgtcatcccg gcaagcatgg
      721 cgcgccagtc aggcctcccc atctcacgcg ccagtctgag ggcaaaattc agctcgccgt
      781 cgaagacttt cccgcagaaa aatcatcatc agtcagcgcg ttatttttcg ccacttcggt
      841 aatatccgta tccacatgaa caggcccgct catcccggac agacgcaaca ccacatcttc
      901 cgcccgggca atggcatcag caggccaggt ggtcaggact tcctgctcaa tctgcatcac
      961 agcctcattc attgacggtg accccgtttt ctgcggatgg ttatgccaca gggacatcgc
     1021 caccagaaac gcgccggttc tgacgagatc ttccacgctt acctgcaggt tgccgctgga
     1081 ttctgcctgt tctgcacgcc gtttcaggag ggcaagatgc tcaatacgct gcagcgcaga
     1141 caattcggaa agcgtgacag acacaccgtt atattcaaat tgttctgttt tcagaaacat
     1201 gtatgacctc cgtttaccct gcagcgcctg cttcagtaac ggtgacttca gctaccgtgg
     1261 caaactgacc attaccggaa atcacgggga tactcacttt tccagcctta acccccgtca
     1321 cagtgatcac catatctttc acagcaatgg ttcccgttga cggatcggcg gaaaccgctc
     1381 tgaacgtctt gtcggttgca ctttccggct caaaagaaac agtcagggtg gttgtttttc
     1441 ctttttccac ggtaccggat gtcggtgtca ccttaattgc agtggccggc gtaatttcgc
     1501 tgcgttcttc cgccacggaa ggtttaccca cgttggtcac tttcaccgta cgggtgatca
     1561 cttccttcgc cgttacggcc ttaccgatac tgctgaccca gccacggaac acatccaccg
     1621 tgccgttcgg gaaacggatt ttataggccc gcacatcccc gctttcaaac cagcctataa
     1681 gccctttctg accttcttct cccggtttcc aggccagcgt aaaactggta tctcctgcag
     1741 acttctgccc ctgcccggtc gcgctccagt ccgcgtcttc atcatccagg tagttatcat
     1801 cgtaggattc tgccgtcatc tcgcccggcg tcagatcctt caccttagcc agtcgctgcc
     1861 agtcatcatc tgacaacggg tttgcataag catcaccctt gccggtgtaa acccacagag
     1921 tggtaccggc accttttacc ggcgccaggg gatttggtgt tggcatatcg tcctcacatc
     1981 tcgtatgtaa tggaataagt caaatctgca gagctccata acgccatatc gtcatcacga
     2041 cgatagtcat agccctgctg aaccatcgtg gtaatcatcc ctgccagtgc agggatcgcg
     2101 gacatcgccg gataaatccg ggactccatc cacgaatcca gctctgaatc cggcacctga
     2161 gcaggcagga aaacttcaat atgcagtgtg gcccgccagg tatccgcatc cagctcttca
     2221 ccggtatact ctgcatccgt cagataaacc gcgaccgcgg gaaaatcctc ttcgtcaaaa
     2281 acaacggggc gaccatcaaa cagcgtcgcc ccgtgttcat gcagctccag tgcatccagc
     2341 actgc
//
LOCUS       DAZFXT010000280         2345 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.283, whole genome shotgun sequence.
ACCESSION   DAZFXT010000280 DAZFXT010000000
VERSION     DAZFXT010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2345
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.283"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..348)
                     /locus_tag="ACHKW0_005096"
     CDS             complement(<1..348)
                     /locus_tag="ACHKW0_005096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7477735.1"
                     /translation="MSQPVGDLVIDLSLDAVRFDEQMSRVRRHFSGLETDARKTVGVV
                     EQNLSRQALAAQKAGISVGQYKAAMRTLPAQFTDIATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAG"
     gene            complement(329..742)
                     /locus_tag="ACHKW0_005097"
     CDS             complement(329..742)
                     /locus_tag="ACHKW0_005097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HFO7477736.1"
                     /translation="MGRPDWRAMLAGMTSTEYADWRHFYRTHYFHDTQLDMHFSGLTY
                     AVLSLFFCDPDMHPSDFSLLAPRREKAQTEMPDEEKMLMQKAAGLAGGVRFGGDGGRE
                     ILSSADVADVSEDDVALMMASAGIPGGVRYVPAGW"
     gene            complement(769..1200)
                     /locus_tag="ACHKW0_005098"
     CDS             complement(769..1200)
                     /locus_tag="ACHKW0_005098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HFO7477737.1"
                     /translation="MFLKTEQFEYNGVSVTLSELSALQRIEHLALLKRRAEQAESSGN
                     LQVSVEDLVRTGAFLVAMSLWHNHPQKTASPSMNEAVMQIEQEVLTTWPADAIARAED
                     VVLRLSGMSGAVHADTDSTEVAKYNALTDDDFSAGKSSTAS"
     gene            complement(1214..1966)
                     /locus_tag="ACHKW0_005099"
     CDS             complement(1214..1966)
                     /locus_tag="ACHKW0_005099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477738.1"
                     /translation="MSTPNPLEPVKGAGTTLWVYNGQGDAYANPLSDDDWLRLAKVKD
                     LTPGEMTAEPYDDNYLDDEDADWTATGQGQKSAGDTSFTLAWKPGEEGQKGLIGWFES
                     GDVRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGKPSVAEERSKITP
                     VTAIKVTPTSGTVAKGKTTTLTVSFEPESATDKTFRVVSADPSKATISVKDMTITVNG
                     VATGKVQIPVVSGNGQFAAVAEVTVTEAGAAG"
     gene            complement(1974..>2345)
                     /locus_tag="ACHKW0_005100"
     CDS             complement(1974..>2345)
                     /locus_tag="ACHKW0_005100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail U family protein"
                     /protein_id="HFO7477739.1"
                     /translation="AVLDALELHEHGATLFDGRPVVFDEEDFPAVAVYLTDAEYTGEE
                     LDADTWRATLHIEVFLPAQVPDSELDSWMESRIYPAMTAIPALADLITTMVTQGYEYR
                     RDDDMALWSSADLTYSITYEM"
BASE COUNT          516 a          746 c          545 g          538 t
ORIGIN      
        1 accggcaagt cccctgaaca tggggatcat cccgccgaag gagtccttca cctgaccgcc
       61 ctgttgcagc aggatcagcc agggattctg accaccggca agctgcgtgg cgatatccgt
      121 aaactgtgcg ggcagggttc gcatggccgc tttatactgc ccgacggaaa tcccggcttt
      181 ttgtgcagcc agcgcctgac gactcaggtt ctgctcaacg acaccgacgg tttttctggc
      241 gtcagtctcc agtccggaaa aatgacgcct tacccggctc atctgctcat cgaaacggac
      301 cgcatccaga ctcaggtcaa taacaagatc accaaccggc tgggacatat ctcacacctc
      361 ccggaatccc cgctgaagcc atcattaatg cgacatcatc ctcgctgaca tccgccacat
      421 ccgcagacga taaaatctca cgccctccgt ccccaccaaa ccgtacgcct ccggcaagtc
      481 ctgccgcttt ctgcatcagc attttttcct catccggcat ctccgtctgc gctttctcac
      541 gccggggggc aagcagactg aaatcagagg gatgcatatc cggatcgcaa aaaaacaggc
      601 tgagtacggc gtacgtcagc ccggaaaaat gcatatccag ttgggtatcg tgaaaataat
      661 gcgtacggta aaaatgtcgc cagtcggcat attcggtgga tgtcatcccg gcaagcatgg
      721 cgcgccagtc aggcctcccc atctcacgcg ccagtctgag ggcaaaattc agctcgccgt
      781 cgaagacttt cccgcagaaa aatcatcatc agtcagcgcg ttatatttcg ccacttcggt
      841 gctgtcagta tccgcatgaa cagccccgct catcccggac agacgcaaca ccacatcttc
      901 cgcccgggca atggcatccg caggccaggt ggtgagcacc tcctgttcga tctgcatcac
      961 agcctcattc attgacggtg atgccgtttt ctgcggatgg ttatgccaca gggacatcgc
     1021 caccagaaac gcgccggttc tgacgagatc ttccacgctt acctgcaggt tgccgctgga
     1081 ttctgcctgt tctgcacgcc gtttcaggag ggcaagatgc tcaatacgct gcagcgcaga
     1141 caattcggaa agcgtgacag acacaccgtt atattcaaat tgttctgttt tcagaaacat
     1201 gtattacctc cgtttaccct gcagcgcccg cttcagtaac ggtgacttca gccactgcgg
     1261 cgaactgacc atttccgctc accacaggga tctgcacctt acctgtcgcc acgccgttta
     1321 ccgtaattgt catatctttc acactaatgg tggctttcga cggatcggcg gaaaccactc
     1381 tgaacgtctt gtcggttgca ctttccggct caaaagaaac cgtcagggtg gttgttttcc
     1441 cttttgccac cgtaccggat gtcggcgtca ccttaatcgc agtgaccggc gtaattttgc
     1501 tgcgttcttc cgctacagaa ggtttgccca cgttggtcac tttcaccgtg cgggtgatca
     1561 cttctttcgc cgtcacggcc ttaccgatac tgctgaccca gccacgaaac acatccaccg
     1621 tgccgttcgg aaaacggatt ttataggccc gcacatcccc gctttcaaac cagcctataa
     1681 gccctttctg accttcttct cccggtttcc aggccagcgt aaaactggta tctcctgcag
     1741 acttctgccc ctgcccggtc gcggtccagt ccgcgtcttc atcatccagg tagttatcat
     1801 cgtagggttc tgccgtcatc tcgcccggcg tcagatcctt caccttagcc agtcgcagcc
     1861 agtcatcgtc tgacaacggg tttgcatagg cgtcaccctg accgttataa acccacaggg
     1921 tggtaccggc accttttacc ggctcaaggg gatttggtgt tgacatatcg tcctcacatc
     1981 tcgtatgtaa tggaataagt cagatccgca gaactccata acgccatatc gtcatcacga
     2041 cgatactcat agccctgcgt aaccatcgtc gtaatcaggt ctgccagtgc cgggatcgca
     2101 gtcatcgccg gataaatccg gctttccatc cacgaatcca gctccgaatc cggtacctgt
     2161 gctggtaaaa acacctcaat atgcagtgtg gcccgccagg tatctgcatc cagctcttca
     2221 ccggtatact ctgcatccgt cagataaacc gcgacggccg gaaaatcctc ttcgtcaaaa
     2281 acaacggggc gaccatcaaa cagcgtcgcc ccgtgttcat gcagctccag tgcatccagc
     2341 actgc
//
LOCUS       DAZFXT010000281         2295 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.284, whole genome shotgun sequence.
ACCESSION   DAZFXT010000281 DAZFXT010000000
VERSION     DAZFXT010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.284"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(34..>2295)
                     /locus_tag="ACHKW0_005101"
     CDS             complement(34..>2295)
                     /locus_tag="ACHKW0_005101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7477740.1"
                     /translation="LTNENGSHSDFSYDALDRLVQQGGFDGRTQRYHYDLTGKLTQSE
                     DEGLVTLWYYDESDRITHRTVNGEPAEQWQYDDHGWLTDISHLSEGHRVAVHYGYDDK
                     GRLTGERQTVENPETGELLWQHETTHAYNEQGLANRVTPDSLPPVEWLTYGSGYLAGM
                     KLGDTPLLEYTRDRMHRETVRSFGSMAGSNAAYKLTSTYTPAGQLQSQHLNSLVYDRA
                     YGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIPYATDPAGNRLPDPE
                     LHPDSTLTAWPDNRIAEDAHYVYHYDEYGRLTEKTDLIPAGVIRTDDERTHHYHYDSQ
                     HRLVFYTRIQHGEPLVESRYLYDPLGRRMAKRVWRRERDLTGWMSLSRKPEMTWYGWD
                     GDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSLAEKLQQEGSEDGHG
                     VVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQVEPEYTPARKVHFY
                     HCDHRGLPLALISEDGNTAWRGEYDEWGNQLNEENPYYLHQPYRLPGQQHDEESGLYY
                     NRNRYYDPLQGRYITQDPIGLAGGWNLYNYPLNPIIRMDPLGLYNLYQLLYDVWHDDS
                     YGTSSIDITGSGDLISLGGHAGLGVAFAKKKGEMLSDICIYATACGHAGIGGGINAAI
                     TYSETKSLPTSGVSNSVGVTVGGGVGGHFAYTYVVDVDNPESSTESVGIGAGVDASVM
                     TLACRTWQECWVN"
BASE COUNT          418 a          729 c          587 g          561 t
ORIGIN      
        1 ctaaaaatat atattttttc atatttcctc ctgttaattg acccagcatt cttgccacgt
       61 tctacaagcc agagtcataa ctgaagcgtc aacacctgca ccgataccaa cagattctgt
      121 cgatgattct ggattatcaa catccactac ataagtatac gcaaaatgcc ccccaactcc
      181 gccgccaacc gttacaccta ctgaattgct gactcccgat gtaggtaaag acttggtctc
      241 agaatatgtg atagccgcat ttatcccacc accaattcct gcatgtccgc atgctgtagc
      301 ataaatacaa atatcagata acatttcacc tttcttttta gcaaacgcaa cgccaagtcc
      361 tgcatgacca cctaatgata ttagatctcc actgccagta atatcaattg atgatgttcc
      421 atatgaatca tcatgccaaa catcatataa taattgatat aaattataca aacccaaagg
      481 atccatcctt attatcggat tcagtgggta attatacagg ttccatcccc ccgccagccc
      541 aatggggtct tgtgtaatat acctcccctg tagcgggtca tagtaccggt tccggttata
      601 gtacagccct gattcctcat catgctgctg ccccggcaga cggtatggct ggtgcaggta
      661 atacgggttc tcttcattaa gctggttgcc ccattcatca tactccccgc gccacgccgt
      721 attgccgtct tcgctgatga gcgccagcgg caggccccgg tggtcgcagt ggtaaaaatg
      781 aacttttcgc gccggtgtgt attccggctc cacctgtctg gccagttgct ccaccgtcag
      841 cccgcactgc gcaagccacg cccggctttc actgctcacg cggtctgccc ggatttcttc
      901 ctccagcctg tccagcagcc gcaccagttc agccggaaat accacgccgt gcccgtcctc
      961 gctcccttcc tgctgcagct tctccgccag gctgcggcgc tgcgctttct cccgctcgcc
     1021 gttgtctgtt tcgatgcgga tgagcggcgc gaagctcccc ggctgatata ccgtctggat
     1081 acgcgtggtg tcggtctgta ccgtcgtcag ccggtcgccg tcccacccgt accacgtcat
     1141 ctccggttta cgcgacagcg acatccaccc cgtcaggtca cgctcccgcc gccatacccg
     1201 tttcgccatt cgccgtccca gcgggtcgta gaggtagcgg ctctcgacca gtggctcgcc
     1261 atgctgtatc cgcgtgtaga acaccagacg gtgctggctg tcgtagtggt agtggtgggt
     1321 ccgctcgtcg tccgtccgga taacacccgc cgggataagg tccgtcttct ccgtcagtct
     1381 gccgtattca tcatagtggt aaacatagtg cgcatcctct gcgatgcggt tatccggcca
     1441 cgccgtgagg gtactgtccg ggtgcagctc cgggtccggc aggcggtttc ccgccgggtc
     1501 ggtggcatac gggatgcgga tgtccaggtc cggtgcgagg gtacgcacgc tctccagcct
     1561 gcccgtggcg ctgtagccgt attcccgcgt ctgtcgcggg ccgctgatgc gcaccaggtc
     1621 gccgttgtca ttccacccgt aggcacggtc atacaccagg ctgttcaggt gctggctctg
     1681 taactggcct gcgggggtgt atgtgctggt cagtttatat gcggcattac tgcctgccat
     1741 gctgccaaag ctgcgcaccg tctcccggtg catgcggtcg cgcgtgtact ccagcagcgg
     1801 cgtgtcgcca agcttcatgc ccgccagata accgctgccg taggtcagcc actccaccgg
     1861 cggcaggctg tccggcgtga cgcggtttgc cagcccctgt tcgttgtagg cgtgtgttgt
     1921 ctcatgctgc cacagcagtt cccccgtctc cgggttctcc accgtctgcc gttcgccggt
     1981 caggcggccc ttatcgtcgt agccgtagtg gacagcgaca cggtggcctt cgctcaggtg
     2041 gctgatgtct gtcagccagc cgtggtcatc atactgccac tgctctgccg gttcgccgtt
     2101 caccgtgcgg tgagtgatac ggtccgattc atcgtagtac cagagggtga caagcccctc
     2161 atcttcactc tgcgtgagtt ttccggtcag gtcataatga taacgttgcg tccgcccgtc
     2221 aaagccgccc tgctgtacca accggtccag cgcatcgtag ctgaagtcgc tgtggctgcc
     2281 gttctcgtta gtcag
//
LOCUS       DAZFXT010000282         2231 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.285, whole genome shotgun sequence.
ACCESSION   DAZFXT010000282 DAZFXT010000000
VERSION     DAZFXT010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.285"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            127..699
                     /locus_tag="ACHKW0_005102"
     CDS             127..699
                     /locus_tag="ACHKW0_005102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1076 domain-containing protein"
                     /protein_id="HFO7477741.1"
                     /translation="MPGLISYVSSASFVNEMMELRQQVMEGQIGGFLLGGERVRVSYM
                     PDTGRFLAESEGRGRVYAELLNIAFNDGVNVLRNRILSALPGMGGRNSLQEKISECAF
                     TVDIEKLQCPGDALQCPITLEQPENGVFIKNSGNSAVCTLFDSVSFSHLVREGGVHPL
                     TRETITPSMIVKPEDCIFDSSKGNFIIKDG"
     gene            917..1615
                     /gene="nleD"
                     /locus_tag="ACHKW0_005103"
     CDS             917..1615
                     /gene="nleD"
                     /locus_tag="ACHKW0_005103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector zinc metalloprotease NleD"
                     /protein_id="HFO7477742.1"
                     /translation="MRPTSLNLALHQSSTSSSMSDADIESLVKTSSVQWIKNNPQLRF
                     QGTDHNIYQQIEAALDKIGSTETGRVLLNAIESISRLKSETVVIHLNSSRLGVIAHRD
                     IDAENHRGTGSDFHCNLNAVEYPCGEGISVVDFHATIVFHELLHVFHNLNGERLKVES
                     SRPESQKYSPLLLEEARTVGLGAFSEEVLSENKFREEIGMPRRTSYPHDSALIHDDNT
                     VSQGFLQVRLHPLL"
BASE COUNT          648 a          416 c          538 g          629 t
ORIGIN      
        1 ggcggaatga aatagccgac agcttcacaa aaaccggagt ccggctccgg tttttgttgt
       61 catgtcatgg tgatgtttgt taataaagac ttgagggaat atttatccgt atgaaggaat
      121 atggtaatgc ctggattaat atcatatgtg tcatcggctt cattcgtgaa tgagatgatg
      181 gagctgcgtc agcaggtaat ggaggggcag attggtggat ttctcctggg aggggagagg
      241 gttagagttt cttatatgcc agatacaggc cgttttttag cagaaagtga agggcgggga
      301 cgggtttatg cagaattatt gaatattgct tttaatgatg gagttaatgt gctcagaaac
      361 aggatattaa gtgcgctccc tggaatggga gggcgaaact ctttgcagga aaaaatatcg
      421 gagtgtgcct ttactgtcga tattgaaaaa cttcaatgtc ctggtgatgc gcttcaatgt
      481 ccaattacac tggagcagcc tgaaaatggt gttttcatta agaattcagg aaattctgcg
      541 gtttgcaccc tgtttgattc ggtttctttt tctcatttgg tccgtgaagg tggggttcac
      601 ccactgacac gagagacaat aactccatca atgattgtaa agccagaaga ttgtatattt
      661 gacagttcaa agggaaactt cattataaaa gatggttaaa atgttttaca tgaagcaata
      721 ttgattttcg gtagtttata taaaacacca cagacattca aaatgtcaga ggatgtcagt
      781 ggtggatgta cctggagcgg aagttgtgga agtaagttat gttatatcgc aataaaccga
      841 atacatctgt gatgttacat tggtctgagc ttcagtgtgt ggtgaatgaa actcgtaaca
      901 ttttcagagg tccttgatgc gccctacgtc cctcaacttg gcattacatc agtcatcaac
      961 gtcgagttca atgtcagatg cagatatcga gtctcttgta aaaacatcga gcgttcaatg
     1021 gataaaaaac aatccgcaac ttcgtttcca ggggactgat cataatatat atcaacagat
     1081 tgaagcagcg ctcgataaga ttggctctac agagacaggg cgtgtactcc tgaatgctat
     1141 tgaatcaata tcccgactta aatcagaaac agtggtaata cacctcaatt cttccagact
     1201 aggagttatt gcacatagag atatagatgc tgagaaccat cggggaactg gttccgattt
     1261 tcactgtaat ctgaatgcag ttgaatatcc ctgtggggag gggattagcg tggtggactt
     1321 tcatgcgact attgtttttc atgagctgct ccatgtgttc cacaatttaa atggggagcg
     1381 cctgaaagtt gagagttccc gaccagaatc acaaaaatac tctccacttt tactcgaaga
     1441 agccagaact gttgggttgg gggctttttc agaggaggtg ctttcagaaa ataaattccg
     1501 cgaagagatt gggatgcccc gtagaacatc ctaccctcac gactcagctc tcattcatga
     1561 tgacaataca gtgagtcagg gattcctaca ggtaagactg catccattgc tttaggtgtg
     1621 tttcttgtgg tgaatatata ttggtgggat aaaactgaga tacataaggt ctcacaatgg
     1681 cttgcaaggc tttgtgcttc tctgtagagg cgacatgttt ttatccggtg cgccgtaaaa
     1741 ccccgtcctt cagggcgtgg aggatgtcaa ataataagga atagtggata tgtttgcagc
     1801 aaagcccgag agcagtcaaa gaagtatttc tggattattc agtcctaggt caaatggtga
     1861 aaacgcaagt aataaaccgt caagtggtat ggcgaacaga atcaaaccag ttgtaagcga
     1921 ttcacaaatc ggtagatata caactgacag taatactctg aaggatggat tgtgtagcca
     1981 agcccacata ggaactcaac cttatcggcc aggtgctgac tcgccgctaa ctgtgagatc
     2041 attcctacaa attacacttt ccaacaataa ttacaaatca tgagtctgcc ctgtcccttc
     2101 tgccaggaag tgttacgcaa cgatatctca ccaatctttc gcaccactat taatcaatga
     2161 gttagaggtg attagtgacc tgagacagag cattagcgca aggtgatttt ttgtccgtaa
     2221 gcgtaaaccg a
//
LOCUS       DAZFXT010000283         2223 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.286, whole genome shotgun sequence.
ACCESSION   DAZFXT010000283 DAZFXT010000000
VERSION     DAZFXT010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.286"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            54..551
                     /gene="rrrD"
                     /locus_tag="ACHKW0_005104"
     CDS             54..551
                     /gene="rrrD"
                     /locus_tag="ACHKW0_005104"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HFO7477743.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMPGKTYTEAECKALLNKDLATVARQINPYINVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKSACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            548..985
                     /locus_tag="ACHKW0_005105"
     CDS             548..985
                     /locus_tag="ACHKW0_005105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7477744.1"
                     /translation="MSRVTAIIYVLVICLIVCLSWAVNHYRDNAIAYKEQRDKATSII
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKRLQVSATCAKSTTGASSM
                     GDGESPGLTADAELNYYRLRGGIDRITAQVNYLQEYIRTQCLK"
     gene            1135..1752
                     /locus_tag="ACHKW0_005106"
     CDS             1135..1752
                     /locus_tag="ACHKW0_005106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HFO7477745.1"
                     /translation="MLASLGGLVSCAAKVHFKEAGNAMNELIANHDFDFRQLVTAAEG
                     QPVTDTFQIARAFGKRHQHVIRAIKCLRCSEEFSTTHFWAVEKINDLGIFDKKQIYYR
                     MDFSGFVMLVMGFNGAKADAVKEAYINAFNWMSAELRKYSESYEAERNAIMLEYMKEK
                     DVASMSGRLLNRWGRTKKPQLLAKLERLERQGQFLLPGFDKGIQA"
     gene            complement(1940..2134)
                     /locus_tag="ACHKW0_005107"
     CDS             complement(1940..2134)
                     /locus_tag="ACHKW0_005107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7477746.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDG"
BASE COUNT          591 a          498 c          571 g          563 t
ORIGIN      
        1 aatctttatt tcaagattaa agaagataag cgtaaggctg cgagaggtga ataatgtcgc
       61 cgtcattacg caaggctgtt gcagctgcta ttggtggtgg ggctgttgcc atagcgtctg
      121 tgctcatcac tggtccaggt ggtaacgatg gtctggaagg tgtcagctac ataccataca
      181 aagatatcgt tggcgtatgg actgtatgtc acggacacac cggaaaagac atcatgcccg
      241 gtaaaacgta taccgaagca gaatgcaaag ccctcctgaa taaagacctt gccacggtcg
      301 ccagacaaat taacccgtac atcaacgtcg atataccgga aacaacgcgc ggcgctcttt
      361 actcgttcgt ttacaacgtg ggcgctggca atttcagaac atcgacgctt cttcgcaaaa
      421 taaaccaggg cgatatcaaa agcgcatgtg atcagctacg gcgctggaca tacgctggcg
      481 gtaagcaatg gaaagggctg atgactcgcc gtgagattga gcgtgaagtc tgtttgtggg
      541 ggcaacaatg agcagagtaa ccgcgattat ctacgttctg gtcatctgcc tcatcgtctg
      601 cctttcatgg gctgttaatc attaccgtga taacgccatc gcctacaaag agcagcgcga
      661 taaagccaca tccatcatcg ctgatatgca gaagcggcaa cgtgatgtag cagaacttga
      721 cgccagatac acaaaggagc ttgctgatgc taatgcgact atcgaaagtc tccgtgctga
      781 tgtttctgct gggcgtaagc gcctgcaagt ctccgccacc tgtgcaaagt caacgaccgg
      841 agccagcagc atgggcgatg gagaaagccc aggacttaca gcagatgctg aactcaatta
      901 ttaccgtctc cgaggtggaa tcgacaggat aaccgcgcag gttaactacc tgcaggaata
      961 catcaggacg cagtgcttaa aataatttta atttcactga aatttaacaa gtgactttca
     1021 ggaaaatgcc tcgcagatgc ggggcatttt tgtaccggta tttcaccgcg caccgcagcg
     1081 cacaataaac accgaacctg accctttgga atgggccttt gaggatacca gttagtgctg
     1141 gcgagcctcg gtgggctggt ttcctgtgcg gcaaaggttc atttcaaaga agcaggcaac
     1201 gccatgaatg aattaattgc gaatcatgac ttcgactttc gccagttagt taccgcagca
     1261 gaaggtcaac cggtaactga caccttccag attgccaggg catttggtaa acgccatcag
     1321 catgtgatta gggctattaa atgtttgaga tgttctgagg aattctcgac aacccatttt
     1381 tgggccgtcg agaaaatcaa tgacttaggg atttttgaca agaaacagat ttactaccgc
     1441 atggacttta gtggcttcgt tatgctggtt atgggattta acggggcaaa agccgatgct
     1501 gttaaagaag cctatatcaa tgcgtttaac tggatgtcag cagaactccg taagtacagc
     1561 gaaagttatg aagcagaacg caacgccata atgctggagt atatgaaaga gaaggatgtc
     1621 gccagcatgt caggccgtct gctcaatcgc tgggggagaa cgaaaaaacc tcaattgctt
     1681 gcaaagctgg aacgtctgga gagacaggga cagtttttat taccgggatt cgataaaggt
     1741 attcaagcct gacacattat gcgctgtatc gtcgccgtat tcccgcatta accatgaccg
     1801 tagcccgacg gggaattcct tctgcgtgag tgtgcgggaa taatcaaaaa cgatgcacac
     1861 cgggttttac tgtgctgaca gacgcagggt taccctcata gtcgcttttc cggtgcgatg
     1921 gtggaagaaa ccgggatgtt catccatcat cactttggat tgatgtatat gctctctttt
     1981 ctgacgttag tctccgacgg caggcttcaa tgacccaggc tgagaaattc ccggaccctt
     2041 tttgctcaag agcgatgtta atttgttcaa tcatctggtt tggaaatcgg atgttgcggg
     2101 ttgttgttct gcgggttctg ttcttcgttg acatgaggtt gccccgtatt cagtgtcgct
     2161 gatttgtatt gtctgaagtt gtttttacgt taagttgatg cagatcaatt aatacgatac
     2221 ctg
//
LOCUS       DAZFXT010000284         2195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.287, whole genome shotgun sequence.
ACCESSION   DAZFXT010000284 DAZFXT010000000
VERSION     DAZFXT010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.287"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            196..2181
                     /gene="yjcS"
                     /locus_tag="ACHKW0_005108"
     CDS             196..2181
                     /gene="yjcS"
                     /locus_tag="ACHKW0_005108"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HFO7477747.1"
                     /translation="MNNSRLFRLSKIIIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          587 a          577 c          524 g          507 t
ORIGIN      
        1 gctcatatta ttttctgcaa tgatagtttt actgtaactt ttcctcttca gcacaaatgc
       61 ctaataaaat ggatcgttca gctcacactt ccacttatta aagtcgcaaa ttcccaatta
      121 tgataacccc agctttaaat gacgtatttt gcatggatta catatttacg acataaatga
      181 taaatggaga tttatatgaa taactctcgg ttattccgtt tgagcaaaat tattattgcg
      241 ttaactgccg ccagcggcat gatggtaaat accgcttacg caacagatga agcgaaagcc
      301 gccactcaat atacccaaca ggttaatcag aattacgcca aatcattacc gtttagcgat
      361 cgtcaggatt ttgacgacgc tcagcgtgga tttatcgctc cgctgctgga tgaaggtatt
      421 ctgcgcgatg ctaatggcaa accatattat cgcggggaag attataaatt tgatatcaat
      481 gcgcccgcgc ccgaaaccgt taaccctagc ctgtggcgtc agtcgcagtt aaacggtatt
      541 tccggcctgt ttaaagtcac cgacagaatg taccaggttc gcggtcagga tatctccaac
      601 atcaccttta tcgaaggtga taccgggatt attgtcatcg acccgctggt gacgccaccg
      661 agcgcaaaag ccgcccttga cctttacttc caaaatcgcc cgcaaaaacc gattgttgcg
      721 gttatttata cccacagcca taccgaccac tatggcggcg tgaaaggcat tatctctgaa
      781 gccgatgtga aatccggtaa agtgcaggtt atcgcccctg ctggctttat ggacgaagcc
      841 atcagcgaaa acgtactggc gggtaatatt atgagccgcc gtgcacttta ctcctacggc
      901 ctgctgctgg cgcataaccc tcagggtaac atcggcaatg gtcttggcgt aacgctggca
      961 tcgggctacc cgagcatcat cgcaccgaac aaaaccatca ccaaaaccgg tgagaagatg
     1021 attatcgacg gcctggagtt tgacttcctg atgaccccag gtagcgaagc accagccgaa
     1081 atgcacttct atattccggc cctgaaagcg ctgtgtaccg ccgagaacgc cacgcatacc
     1141 ctgcacaact tctacactct acgcggcgcg aaaacccgcg acaccagcaa gtggaccgag
     1201 tatctgaacg aaacgctgga tatgtggggt aacgacgcgg aagtcctgtt tatgccgcac
     1261 acctggccgg tctggggcaa taagcatatc aatgattata ttggtaaata tcgcgatact
     1321 atcaagtaca ttcacgacca gaccctgcac ctggcgaacc agggctacac catgaatgaa
     1381 atcggcgaca tgattaaact gccgcctgca cttgccaata actgggccag ccgtggctat
     1441 tacggttctg tcagccacaa cgcccgcgcg gtgtataact tctatcttgg ctattacgac
     1501 ggtaacccgg ctaacctgca tccgtatggt caggtggaga tgggtaaacg ttacgtgcag
     1561 gcgctgggcg gttctgcccg tgtcatcaac ctggcgcaag aagcgaacaa gcaaggtgat
     1621 taccgctggt cggcagaact gctgaaacag gtgattgccg ccaacccggg tgaccaggtc
     1681 gcgaagaatc tgcaagcgaa taactttgaa cagctgggct atcaggccga gtccgccacc
     1741 tggcgcggtt tctacctgac cggcgcgaaa gagctgcgcg aaggggtgca taagttcagc
     1801 cacggcacca ccggttcccc ggacaccatt cgcgggatgt cggtcgaaat gctgttcgac
     1861 tttatgtccg ttcgcctcga tagcgcgaaa gccgcgggta aaaatatcag cctgaacttc
     1921 aatatgagca atggcgataa cctcaacctg acgctgaacg atagcgtgct taactaccgt
     1981 aaaacactgc aatcccaagc tgacgcctct ttctacatca gccgtgaaga tctgcacgcc
     2041 gtgctgaccg ggcaagccaa aatggcggat ctggtaaaag cgaagaaagc caaaattatt
     2101 ggcaatggcg cgaaactgga agaaattatc gcctgtctgg ataatttcga tttgtgggtg
     2161 aatatcgtaa ccccaaatta atgtcactcg ctttt
//
LOCUS       DAZFXT010000285         2164 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.288, whole genome shotgun sequence.
ACCESSION   DAZFXT010000285 DAZFXT010000000
VERSION     DAZFXT010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.288"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..270)
                     /locus_tag="ACHKW0_005109"
     CDS             complement(<1..270)
                     /locus_tag="ACHKW0_005109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477748.1"
                     /translation="MKTSDNERIKYEITGQAVIQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMFNGKKINRIH"
     gene            complement(313..549)
                     /gene="ycgZ"
                     /locus_tag="ACHKW0_005110"
     CDS             complement(313..549)
                     /gene="ycgZ"
                     /locus_tag="ACHKW0_005110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein YcgZ"
                     /protein_id="HFO7477749.1"
                     /translation="MHQNSVTLDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEHATGEEEQKHYNALIGLLFE"
     gene            863..2074
                     /gene="bluF"
                     /locus_tag="ACHKW0_005111"
     CDS             863..2074
                     /gene="bluF"
                     /locus_tag="ACHKW0_005111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HFO7477750.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCALSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFTMAHALELGDKMISINLLPMTLVNEPDAVSFLLNEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          633 a          445 c          420 g          666 t
ORIGIN      
        1 atgtattctg tttattttct taccattgaa catactttta ttgtcagctg tcgctgttct
       61 tcggtttggt cctcctgaat cactattgct aaaatcacga attatgctgt caattaaatc
      121 gcgtcggaaa gcatcttcct cagccgattt cattacgagt aattgcttaa taagagtctg
      181 caatgaaaaa tttatcttca tgcgcagtat ctggatcacc gcctggccag taatttcata
      241 ttttatacgt tcattatcag atgtcttcat aacgacttca tagctaacct gttaaataat
      301 caggctgttg cattattcaa aaagcaaccc aattagtgca ttataatgtt tctgttcctc
      361 ttccccagtc gcgtgttcca gtcggcaaag aagtttggca caaagcgact ttcgactcag
      421 attacgtcca tctttcaaaa tctcagtcac aatttccccg agagtttcct gctgagtagg
      481 caagttagct tttgcaaagt aacgagtgat tgctcccgca gaatctaaag tcactgaatt
      541 ttgatgcatg ctacgcctct gttaaaaatg ttaatcaatg ttaagtgagg taacaatagc
      601 aaacttgtac gaaatatgtg tacagaatga taaattaatt ttttgcaatt agtgctaatg
      661 catataaaac aatgtgttat gataaaacat attgcatcat ttacctgaca atctatacaa
      721 gatttttcgc caactagtgt tcaacttttt ccatttttta tcaatgtaaa gaaacggtta
      781 tgttttggta ttaaaagaaa gacacaagaa gatatgtctg ttaccgtctt actctcgcct
      841 cacccattac cctggattgt taatgcttac cacccttatt tatcgtagcc atatacgtga
      901 cgacgaacct gtcaaaaaaa tcgaagaaat ggtttcgata gcaaatcgca ggaacatgca
      961 gtctgacgta acagggatct tactgtttaa tggttctcat tttttccagc ttctggaagg
     1021 tccggaagaa caggttaaaa tgatatatcg ggctatatgc caggatccac ggcactataa
     1081 tattgttgag ctgctgtgcg attacgcgcc tgctcgccgt tttggcaaag cgggaatgga
     1141 attatttgat ttgcgcctgc acgagcgaga tgacgtttta caggccgtat tcgacaaagg
     1201 cacatcaaaa tttcagctaa cttatgatga cagagcgcta caattttttc gtacttttgt
     1261 ccttgcaacc gaacaatcaa cctatttcga gatccctgcc gaagactcct ggctttttat
     1321 cgctgacgga tctgataaag aacttgattc ctgtgccctt tcaccaacta taaacgacca
     1381 ctttgccttt catcctattg tcgatccctt atcgcggcgg ataatcgctt ttgaagccat
     1441 tgtgcaaaaa aatgaagata gcccatcagc catagcggtt gggcagcgta aagacgggga
     1501 aatctacaca gcggatctca aaagtaaggc gcttgcattc acgatggcac acgcacttga
     1561 gctcggcgat aaaatgattt caatcaatct attacctatg accctggtta acgaacctga
     1621 cgcagtctct tttttactta atgaaataaa ggccaatgct ctggtgcctg aacaaatcat
     1681 cgttgaattt actgaaagtg aagtcatatc tcggtttgat gagtttgccg aagcgattaa
     1741 atcgctaaag gctgctggta tcagtgtagc aattgatcat tttggcgcag gttttgctgg
     1801 tttgttactc ctgtcacgct tccagcctga cagaattaaa atcagtcagg aattgattac
     1861 caatgttcat aaaagtgggc cacggcaggc aattattcag gcgatcataa aatgctgtac
     1921 atcacttgaa attcaagtca gtgctatggg cgtggcaaca ccagaagagt ggatgtggct
     1981 tgaatccgca ggaattgaga tgtttcaggg agatctgttt gctaaagcta aattgaatgg
     2041 tatcccttcc atagcgtggc cagagaaaaa ataattttca gcacattctt tcacatgatt
     2101 tcagtaaatc atcgtgaaaa tgaatacgtt acgtttaaat ttaatccaca ttaaaaataa
     2161 tgtt
//
LOCUS       DAZFXT010000286         2105 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.289, whole genome shotgun sequence.
ACCESSION   DAZFXT010000286 DAZFXT010000000
VERSION     DAZFXT010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.289"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..605
                     /locus_tag="ACHKW0_005112"
     CDS             <1..605
                     /locus_tag="ACHKW0_005112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7477751.1"
                     /translation="VPADGKSITPDDVRPMIEQMVKEAVSHIPVPRDGRDYDPDVLQK
                     AVLEAVSALPAPQDGRDAMALEILPAIDDQKSFPRGSYATHQGGLWRAYEKTYGMRGW
                     ECLVDGVADIDVSMTGERSFSVVVRQSSGQRTEKTFSLPVMLYRGVFRTGETYHPGDT
                     VTWGGSLWHCNSMTGDKPGEAHSSGWTLAAKRGRDAGGGK"
     gene            602..928
                     /locus_tag="ACHKW0_005113"
     CDS             602..928
                     /locus_tag="ACHKW0_005113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO7477752.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDEMLMDKVRQATAVLLAYIQGSR
                     DKVISEDGELIPGEALTRMKGAAMRLTGMLYRNPDLAEREDLVQGELPFSVSVLIYDL
                     RCPTVL"
     gene            939..1289
                     /locus_tag="ACHKW0_005114"
     CDS             939..1289
                     /locus_tag="ACHKW0_005114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7477753.1"
                     /translation="MAISAGRLTQMISVLNPVLTRNAAGEMTEEWVSCGKIHADIRGR
                     SSRERMQSGAEMAQAEIRIWVRGQSGREITAASRLHVLSGPWRDHVLNVVGVPVPDAT
                     GGRLEILCRLGGEK"
     gene            1286..1732
                     /locus_tag="ACHKW0_005115"
     CDS             1286..1732
                     /locus_tag="ACHKW0_005115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97-gp10 family putative phage morphogenesis
                     protein"
                     /protein_id="HFO7477754.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANELKDE
                     VVSRAPVRRGKLRRNVVVLSWRSRDGGMESGVHIRGVNPDTGNSDNTMKADNPRNAFY
                     WRFVEMGTVNMPPHPFVRPAFDVRSEQAAQVAIARMNRAIDEVLRR"
     gene            1729..2105
                     /locus_tag="ACHKW0_005116"
                     /pseudo
     CDS             1729..2105
                     /locus_tag="ACHKW0_005116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463338.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
BASE COUNT          449 a          491 c          735 g          430 t
ORIGIN      
        1 cggtaccggc agacggtaaa agtatcaccc ccgatgatgt gcgtccgatg attgagcaga
       61 tggtgaaaga ggcagtgagc catattcctg ttccgcgtga tggtcgtgac tacgatccgg
      121 atgttctgca gaaggcggtt ctggaggcgg tgagtgccct gccggctccg caggacgggc
      181 gtgatgccat ggcactggaa atactccccg ccattgacga tcaaaaatcc tttccccggg
      241 gctcgtatgc cacacaccag ggtggactct ggcgggcgta tgaaaaaacg tacgggatgc
      301 ggggatggga atgcctggtt gacggggtgg cggatattga cgtcagcatg acgggtgaac
      361 ggtcgttctc tgtggtggtc cggcagagca gtggccagcg tacggaaaaa acattttccc
      421 tgccggtgat gctctaccgt ggtgtgttca gaaccggcga aacttaccac cccggcgata
      481 cggtgacgtg ggggggctcg ttgtggcact gcaacagtat gaccggtgat aagcccggag
      541 aagctcattc atcaggctgg actctggctg caaaacgtgg gcgggatgca ggaggtggaa
      601 agtgacagca ttactgacac tggaagaaat caaggcacat ctgcgtgttg accatgacgc
      661 ggatgatgag atgctgatgg acaaggttcg tcaggctacc gccgtgctgc tggcctacat
      721 ccagggcagc cgggataaag tgattagtga ggacggtgag ctgatccctg gtgaggcatt
      781 aacccggatg aagggggctg ccatgcgact gaccgggatg ctgtaccgga atccggatct
      841 tgctgagcgg gaagacctcg tgcaggggga actgccgttt tctgtttccg tgctgattta
      901 cgatttgcgt tgtccgacgg tgttatgagg agggggggat ggcaatatct gcaggtcgtc
      961 tgacacagat gatcagtgtt ctgaacccgg tgttaacccg taatgctgcc ggagaaatga
     1021 cggaagaatg ggtgtcatgc gggaaaattc atgcggatat ccggggcagg agcagccggg
     1081 agcggatgca gtccggtgcg gaaatggcgc aggcggaaat ccgcatctgg gtgcgcggtc
     1141 agtctggtcg tgaaatcacg gcggcgtcac gacttcatgt gctgagtggt ccctggcgtg
     1201 accatgtcct gaatgtcgtc ggggtcccgg ttccggatgc aaccggcggg cgtctggaaa
     1261 ttctctgtcg gctgggaggg gaaaaatgat cgaaaccctg ctggattttt cggggctgga
     1321 ggacatcagc cgcgatttgc agcttctgag tggtgcggaa aacaaccggg tgttgcgtga
     1381 ggcaacccgc gcgggggcga atgaactgaa agacgaagtg gtgtcacggg caccggtgcg
     1441 caggggaaaa ctgcgccgca atgtggtggt cctttcctgg cgttcccgcg atggcgggat
     1501 ggaatccggt gtgcatatcc gtggtgttaa tccggacacc ggcaacagtg ataacaccat
     1561 gaaggcggat aatccccgca atgccttcta ctggcggttt gtggaaatgg ggaccgtgaa
     1621 tatgccaccg cacccgtttg tgcgcccggc atttgatgtg cgcagtgaac aggcggcaca
     1681 ggtggcgatt gcgcggatga accgggccat tgatgaggta ctgagacgat gacggaggcc
     1741 atttccacgg ggctgaaaat ctccggtgcc agtacggctg aggcgggttc ggtgatcacc
     1801 cagttcagcc aggcgctggc acagggtgtg ttgcgcggtg aggaatttaa ttcggtcaat
     1861 gaaagtggtg accggatcat tcgtgcactg gctgcaggca tgggcgtggc ccgtaaagat
     1921 ctgaaggcga tggcggacga cggcaaactg acggcggata aagtcgtgcc cgcgttaatc
     1981 agccagctgg ggacattgcg tgatgaatat gccgccatgc cagaaacggt ctctgacggg
     2041 atcacaaagg tggaaaacgc ctttatggcc tgggtgggtg gtgcgaatga ggccagcggg
     2101 gtgac
//
LOCUS       DAZFXT010000287         2101 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.290, whole genome shotgun sequence.
ACCESSION   DAZFXT010000287 DAZFXT010000000
VERSION     DAZFXT010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.290"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..701)
                     /locus_tag="ACHKW0_005117"
     CDS             complement(<1..701)
                     /locus_tag="ACHKW0_005117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7477755.1"
                     /translation="MAFKHYDVVRAASPSDLAEKLTHKLKEGWQPYGGPVAITPYTLM
                     QAVAIEGDPQVGPSSEPDWFYVVVLAGQSNGMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGESCTYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDLIARTKAAL
                     QKNPKNVLLAVCWMQG"
     gene            970..1086
                     /locus_tag="ACHKW0_005118"
                     /pseudo
     CDS             970..1086
                     /locus_tag="ACHKW0_005118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013009199.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1191..1267)
                     /locus_tag="ACHKW0_005119"
     tRNA            complement(1191..1267)
                     /locus_tag="ACHKW0_005119"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1231..1233),aa:Arg,seq:tct)
     gene            complement(1281..1357)
                     /locus_tag="ACHKW0_005120"
     tRNA            complement(1281..1357)
                     /locus_tag="ACHKW0_005120"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1321..1323),aa:Arg,seq:tcg)
     gene            complement(1365..1440)
                     /locus_tag="ACHKW0_005121"
     tRNA            complement(1365..1440)
                     /locus_tag="ACHKW0_005121"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1405..1407),aa:Ile,seq:cat)
     gene            complement(1469..>2101)
                     /locus_tag="ACHKW0_005122"
     CDS             complement(1469..>2101)
                     /locus_tag="ACHKW0_005122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7477756.1"
                     /translation="GSVVLATKCIRALFLMHYGKVKIFDINHSIVSQYLEIQHKLTRT
                     HLTDVPLYLSLEPNNPALAEALITSQRFSGDTTDMFLMMACLSLFESDERILLFLSGC
                     LSSISAKVRAIIQTDISASWTLGAIALRLHMSESLLKIKLKNEGHMFSRLLLEERMRV
                     AVNMLCSRHGYGQAVAEKCGYSSWSYFISVFHRYYGFPPDRYVSRQGLDY"
BASE COUNT          536 a          563 c          501 g          501 t
ORIGIN      
        1 ccctgcatcc agcacaccgc cagcaacaca tttttcgggt tcttctgtaa tgcagcttta
       61 gtgcgcgcaa tcaggtcctg atataacggt ttacccacac cccagcgtgc cgaatcctgg
      121 ctggcccccg tgtccgcact gaatgtcccc tcagcgccct gggtgaatgc agaaccacca
      181 cgacagcatg gtaccagcag gatccccgcg ttattcggga tatacggaag cagttttttg
      241 gcaatatgta agccctggcc gacacagccg tactgccctt tgctcaggtc tgccttcgga
      301 tgattcagcg tactcatatc ctgcacatca tgcaggcagt ggtcagccgg aatgatgtcg
      361 ttatacgtac aactctctcc acccggagtt accgtgctgc ggcgcgccag ctgtttaatg
      421 cgcggatccg gagcatcgta agaatccggt aacggaagcc cttcaccgta ggccatgccg
      481 ttggactgtc cggcaagcac aaccacgtag aaccagtccg gctcagatga agggccgacc
      541 tgtggatctc cttcaatagc caccgcctgc atcagtgtgt acggcgtaat ggcaaccggt
      601 ccgccgtatg gctgccagcc ctctttcagt ttgtgtgtca gcttttccgc aaggtctgac
      661 ggcgacgccg ccctgacaac atcatagtgt ttaaatgcca tgaatcctcc cggccgggat
      721 aatattgtga gtaaaatgag gagcgggctg aagtccggaa gttacaggac aatggcagaa
      781 gagagacaac agcccgcaat acgaaaaagg ccgcgctatt gcgcagagtg attactgtcg
      841 gatattattc gccagctgaa atattacttc acgttttgtt gtttattcct tgccgcccgc
      901 gtctccctgc gcgggctttt tttgtccata agaaagcccc tccggagagg ggctggagag
      961 tggcgctatg tgccattgca tggtgccggg tgcctcccgg tgaattcagt accagcacct
     1021 gaatccgcga ttatcccata tacctactcg ctgattgccc ctccgcacag ggggattcac
     1081 catgccagtt tcttttaaca aactccccgc aaaccagaca acagtcaacc gcctgaattg
     1141 tgagacattt aaaaaaaagg cccgcaaaag cgagccaggg aaaataagtg tggcgcgttg
     1201 tactggattc gaaccagtga ccgattgctt agaaggcaat tgctctgtcc ggctgagcta
     1261 acaacgcagg atacagataa tggaccgcct tcggggaccc gaactccgcg caaccagctt
     1321 cgaaagctgg cgctctttcc tgatgagcta atggcggtat gtgatggtgg cccttgctgg
     1381 atttgaacca gcgacctggc gattatgagt cgctcgctct caccactgag ctaaagggcc
     1441 gggtcaaaaa ataataatca gatgaaaatc aataatcaag cccttgcctg gatacatatc
     1501 tgtctggcgg gaagccataa tagcggtgaa atacagaaat aaagtaggac cagcttgaat
     1561 aaccgcattt ttctgctaca gcctgtccat atccatgccg ggaacataac atattcacag
     1621 caacacgcat ccgctcttcc agcaacaagc gactgaacat gtgcccttca tttttcagtt
     1681 ttatctttaa caaactctca ctcatatgca ggcgtaacgc aatcgcacca agcgtccagc
     1741 ttgctgatat atctgtctga attatcgccc tgactttggc acttatactg gataaacatc
     1801 cacttaaaaa taataatatc cgttcatctg attcaaacag cgacaggcat gccatcataa
     1861 gaaacatatc cgtggtatct ccggaaaatc tctggctggt aattaaagcc tcagccaacg
     1921 cagggttgtt gggttccagt gacagataaa gcggaacgtc agtcagatga gttcttgtca
     1981 gcttatgctg aatttccaga tattgactta ctatggaatg gtttatatcg aaaattttaa
     2041 ctttgccata atgcataagg aaaagcgccc tgatgcattt ggtggccaga acgactgagc
     2101 c
//
LOCUS       DAZFXT010000288         2100 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.291, whole genome shotgun sequence.
ACCESSION   DAZFXT010000288 DAZFXT010000000
VERSION     DAZFXT010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2100
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.291"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..275)
                     /locus_tag="ACHKW0_005123"
     CDS             complement(<1..275)
                     /locus_tag="ACHKW0_005123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477757.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGT"
     gene            complement(336..983)
                     /locus_tag="ACHKW0_005124"
     CDS             complement(336..983)
                     /locus_tag="ACHKW0_005124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7477758.1"
                     /translation="MAATHTLPLASPGMARICLYGDLQRFGRRIDLRVKTGAEAIRAL
                     ATQLPVFRQKLNEGWYQVRIAGRDAGENELSARLNEPLANGAVIHIVPRLAGAKSGGV
                     FQVVLGAALIAVAWWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTD
                     NGKQNTYFSSLDNMVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            complement(881..1624)
                     /locus_tag="ACHKW0_005125"
     CDS             complement(881..1624)
                     /locus_tag="ACHKW0_005125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7477759.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLSAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNMEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLVAVSTFV"
     gene            complement(1630..>2100)
                     /locus_tag="ACHKW0_005126"
     CDS             complement(1630..>2100)
                     /locus_tag="ACHKW0_005126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7477760.1"
                     /translation="PTLTVSNLYGMVTGMAEDMQSLVGGTVVRRKVYARFLDAVNFVN
                     GNSYADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDGAVFPGRIMLANTCTWTYR
                     GDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNVGNFGGFLSINKLSQ"
BASE COUNT          447 a          712 c          519 g          422 t
ORIGIN      
        1 gtaccgagca ccgtctcgga gccggaggat tcaaatccct ccggcggtgt ctgctcctgc
       61 tcaccggccc ggaacaccac cgtgacgccg gatatattgg tattcccctc actgtccagc
      121 accggcgtac tgttcagcag cacgcttttt aatccatcca ccggaccttc aaccggccct
      181 tcgctgatgg catcgatcac actcagcagc tgcgtggact tcaggttgtc cttcgcttcg
      241 cgcggggtat gccccttact gctgccttta cccattcctc acgctccata aacgataaaa
      301 ccgcccggag gcggtttcac ataaaacatt ttgcatcagc gaccaatcac cacaacctga
      361 ccaccatccc cttcgtctgc cgtgctgatc tcctgagaaa ccacacgtga ccccacgcgc
      421 atttcaccgt acagaacagg cagaacattg ccctgggcaa ccatgttatc cagtgaggag
      481 aaataggtgt tctgtttgcc gttatctgta ctggctgccg tgggcgtcct ggctttcggt
      541 gccagcatct gcgccactcc gcccaggatc atactggccc ctgccgcata catgcccgat
      601 acagccgcgg cccccagcca gcccacaggg ttccaccatg ccaccgcaat cagcgccgcc
      661 ccaagcacca cctgaaacac accgccactt ttagctcccg ccagacgcgg cacgatgtgg
      721 atcacggcac catttgccag cggctcatta agacgggcag ataattcatt ttcgcctgca
      781 tcacgcccgg caatgcgcac ctgataccag ccctcattca gtttctgacg aaacaccggg
      841 agctgtgtgg ccagtgcccg gatggcttca gcccccgttt tcacacgaag gtcgatacgg
      901 cgaccaaatc gttgcaaatc cccgtaaagg cagatgcgtg ccatgcccgg tgacgccaga
      961 gggagtgtgt gcgtcgctgc catttgtcgg tgtacctctc tcgtttactc agttgttcag
     1021 gaatatggtg cagcagctcg ccgtcgccac agtaaatggc ggcatgattc ggcaccgatg
     1081 aaccaaaaca gcacagcagc acatcgcccg gctgggccgc tgacaacggc acctgataca
     1141 accccgttgc ctccatatta tccagataga gattctggcc gtgacgccac cagtcatccc
     1201 cgcgatgaaa atccggcatc tcaattcccg ccagatggta agcgtcccgg aacagcgtgt
     1261 aacagtccgt caccccgtgc tcaaagcgcc gcccggtgag atgcggcaca cagcggaatt
     1321 tatgaatcgc cccccggcag accagccacc acggcaaatc actctgcacc tgcagccgcc
     1381 ggtcggcctc actcagccag ggcagaccac cggggtggct gtggaccagc gccacaatct
     1441 ctccctgcat ttctgcactc agccagtcct ccggcgacat ccggaaatac gcctccggct
     1501 caccagagat attcacgcag ggaaaatatc tttccccctc cggtgttctc accacgaagc
     1561 cgcacgactc cgctggcgca catcgccggg cgtgcgccag aatcgctgat tctgtctctg
     1621 tcatgggatt cactgcgaaa gtttgttaat ggaaaggaag ccgccaaagt tgccgacgtt
     1681 attgcgaaac ttacagccgc tcaggcattt gctgcattta tccttcgtga tatcggacgt
     1741 cggctgatca tattcatccg cgaccgccgg accgtgataa ccgcactcgt caccgcgata
     1801 ggtccaggtg caggtgttgg ccagcatgat gcgccccgga aaaacggcac cgtccgtttc
     1861 cgtcggcgtg gacagtacaa aagaggcact caccgcgctc agttcgctgc actgctcgat
     1921 gcgccagcgg ctgatcacct cctgctccgg atcggcgtaa ctgtttccgt tgacgaagtt
     1981 caccgcatcc agaaaacggg cgtaaacctt acgccggacc accgttccgc cgaccagact
     2041 ctgcatatct tccgccatcc cggtgaccat accgtacagg ttagaaaccg tcagcgtggg
//
LOCUS       DAZFXT010000289         2028 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.292, whole genome shotgun sequence.
ACCESSION   DAZFXT010000289 DAZFXT010000000
VERSION     DAZFXT010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.292"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            248..1066
                     /locus_tag="ACHKW0_005127"
     CDS             248..1066
                     /locus_tag="ACHKW0_005127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF932 domain-containing protein"
                     /protein_id="HFO7477761.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMRHVPSIFGENRHTSRSEHY
                     AYIPTITVLENLQQEGFQPFFACQTRVRDQSRREYTKHMLRLRRAGQITGQHVPEIIL
                     LNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESMR"
     gene            1157..1642
                     /locus_tag="ACHKW0_005128"
     CDS             1157..1642
                     /locus_tag="ACHKW0_005128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HFO7477762.1"
                     /translation="MTTVSHNSTTPSVSVTTASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNA
                     MNGNRAEMSPEAAGIAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            1658..>2028
                     /locus_tag="ACHKW0_005129"
     CDS             1658..>2028
                     /locus_tag="ACHKW0_005129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HFO7477763.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALQTLDRHLHEPGVAFTSTRAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVTPSKADRLIT"
BASE COUNT          474 a          562 c          533 g          459 t
ORIGIN      
        1 gttaagatga atttatttaa ttttggggga agatacaaaa acaaccagtg aacatatttc
       61 cggcgggtta tccgcaatgc tgaaggtcag ttgcactggc gatgccggaa tacagcttaa
      121 tacgtggttc gccagtgaag ggcttctcag acaataaacc tcattcattt cacccatcag
      181 gccgcgtctt ctccgggaga cgcggctttt tcatttatat cgcttaatca ttcataagga
      241 gcaaatcatg agattagcca gtcgttttgg ttatgcgaac cagatacgtc gtgatcgtcc
      301 gctgacacac gaagaactga tgcgccatgt accgagtatt tttggggaaa accgacatac
      361 ctctcgcagt gaacactatg cgtacattcc cactatcacc gtcctggaaa atctgcagca
      421 ggaaggcttt cagccgttct ttgcctgcca gacccgcgtg cgcgaccaga gccgccggga
      481 atacacaaaa cacatgctgc gtctgcggcg ggccggacag ataaccggtc agcatgtccc
      541 tgaaattatt ctgctcaact cccatgacgg ttcatccagc taccagatgt tacccggata
      601 ttttcgtgcc atttgtacca atggactggt ctgcggtcag tctctgggag aattgcgtgt
      661 tccacaccgg ggaaatgtgg tggacagagt cattgaaggg gcttacgagg tggtgggcgt
      721 ttttgaccgg atagaggaga agcgtgatgc catgcagtcg ctgattctgc cgccaccggc
      781 acgccaggcg ctggcacagg cggcactgac ttaccgttat ggtgacgaac atcagcccgt
      841 caccaccgcc gacattctga caccacgacg ccgggaggat tacggtaagg acctgtggag
      901 tgcttatcag accatccagg agaatatgct gaaaggcggg atttccggtc gcagtgcaaa
      961 aggaaaacgt atccataccc gggccattca cagcattgat accgacatta agctcaatcg
     1021 cgcattgtgg gtgatggcag aaacgctgct ggagagcatg cgctgatgcc gtttcactga
     1081 aagtgcagtc ctgactcaca tctgttcctg ccccggggct tcttccattc atttactttt
     1141 tataaggaat aacctcatga caaccgtttc gcataattcc accacacctt ctgtttccgt
     1201 aaccactgca tcagggaata acccacctca gctggttgcc actcccgtcc ctgatgaaca
     1261 gcgcatcagc ttctggccgc agcattttgg cctcattcca cagtgggtga ccctggagcc
     1321 ccgtgtcttc ggctggatgg accgtctttg cgaagactac tgcggtggta tctggaatct
     1381 gtacaccctg aacaacggcg gggcatttat ggcacccgaa ccggatgacg atgatgacga
     1441 aacatgggta ctgttcaatg ccatgaacgg caaccgcgct gaaatgagcc cggaagccgc
     1501 cggtattgct gcctgtctga tgacgtacag tcatcatgcc tgtcgtacgg agtgttatgc
     1561 catgacggtc cactattacc ggctgcggga ttacgccctg cagcatccgg aatgcagcgc
     1621 cattatgcgc atcattgact gaatggaggc agggacaatg cagcagcttt cctttctgcc
     1681 cggagagatg acgcccggcg agcgcagtct cattctgcgg gccctgcaaa ccctggaccg
     1741 ccatcttcat gaacccggcg tggccttcac ctccacccgt gcggcacggg aatggctgat
     1801 tctgaacatg gcgggactgg agcgggaaga gttccgggtg ctgtatctga acaaccagaa
     1861 tcagctgatt gccggtgaaa ccctcttcac cggcaccatc aaccgtacgg aagttcatcc
     1921 ccgggaagtg attaaacgcg ccctgtacca caatgccgct gccgtggtgc tggcgcacaa
     1981 tcacccgtcc ggtgaagtca cacccagtaa ggcagaccgg cttatcac
//
LOCUS       DAZFXT010000290         2001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.293, whole genome shotgun sequence.
ACCESSION   DAZFXT010000290 DAZFXT010000000
VERSION     DAZFXT010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.293"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1287..1889
                     /gene="fimB"
                     /locus_tag="ACHKW0_005130"
     CDS             1287..1889
                     /gene="fimB"
                     /locus_tag="ACHKW0_005130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HFO7477764.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          613 a          347 c          409 g          632 t
ORIGIN      
        1 atttaaataa acagagatcc agattaaata cctgagtata aaatctcttc tgatgtttaa
       61 ttgatttgaa tgttcgtaag ctatatcact tactcaatcc attttaccca gagtcataaa
      121 atagaatgtc tgttgatctc attttcatat tcctttcaga ctgaagtgcg ttttcatcac
      181 ccgatgagaa agaactgaag tgatgtttgc catagattgc gagatgccct gtctcattga
      241 ctatcttggt gcacctttat acctgttata ccagatcaaa aatcacgcaa tccatacaac
      301 aaaaccagat ttgcaattcg tgtcacaaaa tatgtcgata tttttctaag aagaagatgc
      361 catgtgaagc cagacgaaca cttgcggagg ccttcacaaa ccaaagattg ttgtttaatt
      421 atctgtttta taagtaattt atttaaagta taaacaagga aagatttgca agttaactca
      481 gtgagcgtgg tgaaaatcag tgtttacccg ccatcaggct gagcataatt ctcatcatga
      541 aatatgtttc ctggtttgtg gcttgtaact ggtcacttct gaagtcgatc tggagaggct
      601 tgttgatgtt ggtgttttca ggatgatgtt tcacttagtt tgtttgccgt atcgcccggc
      661 gaatggctgt gattgaggaa ggtttaagtc gtagtgacca aagctatatt taccaacgaa
      721 tgtagatgaa aaaatcatct cctgcgttcc ccatatctcc aggataaaaa ggaatgtaac
      781 aatctcatgg cgtaagctga cgaatcagcc aggaataatc gctagggacc taagaattag
      841 catgataata gccactaaga aattactgcg ctccatgaaa tagccatttt gtggcaaatg
      901 gagttgacta ataatgtcat atgtgagacg gctagttgaa cgaatattaa attttgctga
      961 attttttatg ttgattttac ttgttacaga acatatcaca tgatatatag ataagattag
     1021 ttgcattaat gatgagggtt attattagat tcgtatccga ttgataaata tataaaggta
     1081 catagcatgc aagagcatgg cgtttgtatg gcaacgttat tataattaac tgttgctact
     1141 ccatttaagt tcactcagaa gaactggtcc acttacgtta gttattaagc aaacgttcgc
     1201 ttttataaac ataatcagga taaaaatgtt ggattattgc taacccagca cagctagtgc
     1261 gcgtctgtaa ttataaggga aaaacgatga agaataaggc tgataacaaa aaaaggaact
     1321 tcctgaccca tagtgaaatc gaatcactcc ttaaagcagc aaataccggg cctcatgcag
     1381 cacgtaatta ttgtctgact ttgctttgtt ttattcatgg tttccgggcg agtgaaattt
     1441 gtcgattgag gatttcggat attgatctta aggcaaagtg tatatatatc catcgattaa
     1501 aaaaaggctt ttcaacaacg catccgctat tgaacaaaga agttcaggct ttaaaaaact
     1561 ggttgagtat ccgtacttcg tacccgcatg ctgagagcga gtgggtattt ttatctcgta
     1621 agggaaatcc gctttctcgg caacagtttt accatattat ctcgacttcc ggtggtaatg
     1681 ccgggttgtc actggagatt catccgcaca tgttacgcca ttcgtgtggt tttgctttgg
     1741 cgaatatggg aatagatacg cgacttatcc aggattatct tgggcatcgc aatattcgtc
     1801 atactgtctg gtataccgcc agcaatgcag ggcgttttta cggcatctgg gatagagcca
     1861 gaggacgaca gcgtcacgct gttttataga gatagttgag ataccaggga tggtgttaaa
     1921 aactacatta ttcttatgat atatcaaaaa tgattaacca tttattaatt gatgttattg
     1981 taagtgtgat tattaatttt c
//
LOCUS       DAZFXT010000291         1994 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.294, whole genome shotgun sequence.
ACCESSION   DAZFXT010000291 DAZFXT010000000
VERSION     DAZFXT010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.294"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(33..>1994)
                     /locus_tag="ACHKW0_005131"
     CDS             complement(33..>1994)
                     /locus_tag="ACHKW0_005131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7477765.1"
                     /translation="IEICDDDYAGISTGGRVLAVNSQTRTLTLDREITLPSSGTTLIS
                     LVDGSGNPISVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIREND
                     DGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVL
                     ARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTAETTYRFTQLAPGNYRLTVRAVN
                     AWGQQGDPASVSFRIAAPAAPSQIELTPGYFQITATPHLAVYDPTVQFEFWFSETRIT
                     DIRQVETTARYLGTALYWIAASINIKPDHDYYFYIRSVNTVGKSAFVEAVGQPSDDAS
                     GYLDFFKGQITESHLGKELLEKVDLTEDNASRLEEFSKEWKDANDKWNAMWGVKIEQT
                     KDGKHYVAGIGLSMEDAEEGKLSQFLVAANRITFIDPANGNETPMFVAQGNQIFMNDV
                     FLKRLTAPTITSGGNPPAFSLTPDGKLTAKNADISGSVNANAGTLNNVTINENCQIKG
                     KLSANQIEGDIVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFSGA
                     KHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPAGHGHMTLEFSVSAW
                     LVNNWYPTASISDLLVVVMKKATAGISIS"
BASE COUNT          411 a          599 c          497 g          487 t
ORIGIN      
        1 catttctggc gcccgtatat gggttataaa attcagctga tactgatgcc tgcggtggct
       61 ttcttcatca ccacaaccag caaatcgctg atacttgctg tgggatacca gttattcact
      121 agccatgctg ataccgaaaa ctccagcgtc atgtgaccgt gaccggcagg catatcaata
      181 acgccactgt aaatcagcgt attatccagc gcggtacggt tataaatttc agcaccgttt
      241 ttccgcacta tcagacggca tgaggagtaa atatcagtat gctctctctc atgcttagcg
      301 ccactgaatg ccaccgccgg aataacaatc tgccggtcaa acggctgatc gtcataaacc
      361 ctgacggtaa tggtccctga tggccaccgc tccggtgccc gggagtcccg cggaaaagcc
      421 ttacccactg ttttgactat atcgccttca atctggttgg ctgacagttt ccccttaatc
      481 tgacagttct cattaattgt gacattgttg agcgtcccgg cgttcgcatt cacactgcca
      541 ctgatatctg catttttagc ggtcagcttt ccgtccggtg tcagggaaaa tgccggagga
      601 ttaccgccgc tggtaatggt gggagccgtc aggcgcttca ggaacacgtc gttcatgaat
      661 atctggttgc cctgcgccac aaacatcggc gtttcattcc cgtttgccgg gtcaataaat
      721 gtgatacgat tggcggcaac cagaaactgg ctcagtttgc cttcctccgc gtcctccatg
      781 ctgaggccaa tacccgcgac ataatgtttg ccgtctttgg tctgctcaat tttgacgccc
      841 cacatggcat tccacttatc gttggcgtcc ttccactctt tcgaaaactc ctccagtctg
      901 ctggcgttat cctccgtcag gtcgactttt tccagcagct ccttgccgag atgggattcg
      961 gttatctggc ctttgaaaaa atccagatag ccggatgcat catcactcgg ctggccaaca
     1021 gcctccacaa atgccgattt gccaacggtg ttcacactgc ggatgtaaaa ataataatca
     1081 tggtccggtt tgatattgat actggcggct atccagtaca gcgccgtgcc aagatagcgg
     1141 gctgtggttt caacctgcct gatatcggta atccgcgttt ccgagaacca gaactcaaac
     1201 tgtaccgtcg ggtcataaac cgcaagatgc ggcgtggcgg ttatctgaaa atagcccggc
     1261 gtcagctcaa tctgtgacgg cgctgccggt gcggcaatcc tgaacgatac cgatgccgga
     1321 tcgccctgct gcccccacgc atttactgcc cggactgtca gcctgtagtt ccccggcgcc
     1381 agttgtgtga agcggtatgt ggtttccgcc gtccgggctg tgctgaccag ccgctcactg
     1441 ccgtcatccg cggccacggt caggcgaagc atgaaactca cgcccttcac caccttcggc
     1501 gtatcccagc gggccagtac ctgatactcc ccgctgtctg cggtgacttc ggcagtcagg
     1561 tgctgcactg ctggcggcgt gacaccattc accgtgccgc tctggtcgcc gtcaaagtgc
     1621 gccccgttat ccacgatggc ttctttttcc ggtacatgct gcacggcggt gatagcatac
     1681 gtgccgtcat cgttctcacg gatactcaca cagcggaaca ggcgctggcg caacgtcggc
     1741 aacttcagcc cccacacgct gtattcagca acaccgtcag gaacacggct cactttcacc
     1801 ttcacgccgt cggtgacgga ctgaacctcc acgctgatcg gattgccact tccgtcaacc
     1861 aggcttatca gcgtggtacc ggaggatggc agcgtgattt cacggtcgag cgtcagcgtc
     1921 cgggtctggc tgtttaccgc cagcacgcgc ccgccggtgc tgataccggc atagtcatca
     1981 tcacagattt caat
//
LOCUS       DAZFXT010000292         1990 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.295, whole genome shotgun sequence.
ACCESSION   DAZFXT010000292 DAZFXT010000000
VERSION     DAZFXT010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1990
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.295"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..399
                     /locus_tag="ACHKW0_005132"
     CDS             <1..399
                     /locus_tag="ACHKW0_005132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7477766.1"
                     /translation="ATVVRRRVYARFLDAVNFVAGNPEADPEQELSDRWVVEQMSELT
                     AMTASFVLATPTETDGALFPGRIMLANTCMWDYRGDECGYHGPAVADEFDNPTTDIRK
                     DRCSKCMRGCEMRGMVANFGGFLSINKLSQ"
     gene            410..1153
                     /locus_tag="ACHKW0_005133"
     CDS             410..1153
                     /locus_tag="ACHKW0_005133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7477767.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVIGTPEGERYQPCVNISAEPE
                     AYFRIAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALPWWLVCRGEIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGITLPDFVREDDWWRNGRNLYLDNMAAT
                     GFYRVPLSSAQAGDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRR
                     THSVWRHRHWHASAFTGICNDLAAASACT"
     gene            1150..1728
                     /locus_tag="ACHKW0_005134"
     CDS             1150..1728
                     /locus_tag="ACHKW0_005134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7477768.1"
                     /translation="MNTAAEAIRALSLQMPGFRRQMNEGWYQIRIRGEDTAPEAVYAR
                     LHEPLGEGAVIHIVPRLAGAGKGGLQIVLGAAAIVGSFFTAGATMALWGAALSAGGLT
                     ATTMLFSLGASMILGGVAQMLAPKAKTPDYRATDNGKQNTYFSSLDNMIAQGNPMPVP
                     YGEMLVGSRRISQDISTRDEGGDGKVVVIGRG"
BASE COUNT          419 a          504 c          658 g          409 t
ORIGIN      
        1 gccacggtgg tccgccgccg ggtgtatgcg cgttttctgg atgcggtgaa ctttgtggca
       61 ggcaatccgg aggccgaccc ggagcaggag ctgagcgacc gctgggtggt ggagcagatg
      121 tcagagctga cggccatgac agcctcgttt gtgctggcaa caccgacgga gacggacgga
      181 gcgctgtttc ccggtcgcat catgctggcg aacacctgta tgtgggatta ccggggagat
      241 gaatgcggtt atcacggtcc tgcggtggcg gatgagttcg acaaccccac cacggatatc
      301 cggaaggaca gatgcagtaa atgcatgcgc gggtgtgaga tgcgcggcat ggtggctaat
      361 ttcggcggtt tcctttccat taataaactt tcgcagtaaa tcccggttta tgacacagac
      421 tgaatcagcg attctggcgc atgcccggcg gtgtgcgcct gcggagtcgt gcggcttcgt
      481 gataggcacc ccggagggcg aacggtacca gccctgcgtg aatatctccg cagagccgga
      541 ggcgtatttt cgtattgcgc cggaagactg gctgcaggca gagatgcagg gggagattgt
      601 ggcactggtc cacagccacc ctggtggtct gccctggctg agcgaggccg accggcggct
      661 gcagataaag agtgccctgc cctggtggct ggtctgccgg ggggaaattc acaaattccg
      721 ctgtgtgccg cacctgaccg ggcgtcgttt tgagcacggg gtgacggact gttacaccct
      781 gttccgggat gcataccatc tggcggggat aacgctgccg gattttgtgc gtgaggatga
      841 ctggtggcgc aacggtcgga acctttacct ggacaatatg gcggcgactg gtttttaccg
      901 ggtgcccctg tcctctgcac aggcgggcga tatcctgctg tgctgctttg gcgcatcggt
      961 ggccaatcat gccgccatat actgcggcaa cggtgaactg cttcaccatc tgcctgaaca
     1021 actgagtaaa cgggagaggt attctgaaaa atggcaacga cgaacgcatt ctgtctggcg
     1081 tcaccgccac tggcacgcat ctgccttcac ggggatttgc aacgatttgg ccgccgcctc
     1141 agcctgtacg tgaacacggc agcggaagcc atccgtgccc tgtcgctgca gatgccggga
     1201 ttccgccgtc agatgaacga aggctggtac cagatacgta ttcgcggtga ggacacggca
     1261 ccggaggcgg tgtacgcccg tcttcacgaa cctctgggtg agggggcggt catccatatt
     1321 gtgccgcgac tggccggagc cggaaagggc ggactgcaga ttgtgctggg ggcagcagcc
     1381 atcgtgggct ctttcttcac cgccggcgca acgatggcgt tgtggggcgc agccctgagt
     1441 gccggagggc tgactgccac cacgatgctg ttctcactgg gtgccagcat gatactgggc
     1501 ggtgtggccc agatgctggc accgaaggca aaaacaccgg attaccgcgc aacggataac
     1561 ggtaaacaga acacgtattt ttcgtcactg gataacatga ttgcccaggg gaacccgatg
     1621 ccggtgcctt atggtgaaat gctggttggt tcacgacgga tatcccagga catcagcacc
     1681 cgtgatgagg gcggagacgg gaaagtggtg gttatcgggc ggggatgaaa ataaaaaaat
     1741 cccgcagagt tagcggagct gcgggagaga acgatgaaga ttaacgttat ggagttattt
     1801 ttcaggcatc aaaaaagtaa tgcagcgtca ttattgcggc tacaggcaat tgccggaaat
     1861 gtgaagagtt tcagaaattt tattccgtca tgacacaggc accctccggg gtgcctgttg
     1921 ttttctggca taaacagatt cagacatcag acaggagagg gggacagagt gggtaaaggg
     1981 ggcggcaagg
//
LOCUS       DAZFXT010000293         1943 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.296, whole genome shotgun sequence.
ACCESSION   DAZFXT010000293 DAZFXT010000000
VERSION     DAZFXT010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.296"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..286)
                     /locus_tag="ACHKW0_005135"
     CDS             complement(<1..286)
                     /locus_tag="ACHKW0_005135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HFO7477769.1"
                     /translation="MAVVCSVIMVCSPINIFLEKDTLSLKPGSVVLATKCIRELFLMH
                     YGKVKIVDISESVVSQYLESQHKLTRTRLTDIPLYLLLEPNNPALAAALIT"
     gene            complement(422..619)
                     /locus_tag="ACHKW0_005136"
     CDS             complement(422..619)
                     /locus_tag="ACHKW0_005136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HFO7477770.1"
                     /translation="MLKQQDMTETARVVFNELSVTEPATVGEIAQNTYLSRERCQLIL
                     TQLVMAGLADYQCGCYRRIQS"
     gene            complement(844..1398)
                     /locus_tag="ACHKW0_005137"
     CDS             complement(844..1398)
                     /locus_tag="ACHKW0_005137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HFO7477771.1"
                     /translation="MIYPEITGKSGEHLRLKTLESVWIQGKLRMWGRWSYIGDGKTGN
                     MFNQLLTSKKLTKTAINEALRRMKKAGLDKPELEAFLRDMINGNQKSWLAHCTDSEAL
                     IIDRVIGEVLAGYPGLLNVLSQRYVGRGMTKRKMAELLNDAHPEWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(1407..1766)
                     /locus_tag="ACHKW0_005138"
     CDS             complement(1407..1766)
                     /locus_tag="ACHKW0_005138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7477772.1"
                     /translation="MRIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVSGGRLGVKIYKIESE"
     gene            complement(1779..>1943)
                     /locus_tag="ACHKW0_005139"
     CDS             complement(1779..>1943)
                     /locus_tag="ACHKW0_005139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7477773.1"
                     /translation="GMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLIFRFL
                     DHAFATGVLG"
BASE COUNT          513 a          522 c          389 g          519 t
ORIGIN      
        1 tggtaattaa agccgcagcc aacgcaggat tgttgggttc cagcaacagg taaagcggaa
       61 tgtcagtcag acgagtcctc gtcagcttat gctgactttc cagatattga cttacgacgg
      121 attcgcttat atcgacaatt ttaactttgc cataatgcat aaggaaaagc tccctgatgc
      181 atttggtggc cagaacgact gagcctggct taagtgacaa cgtatccttt tcaagaaaaa
      241 tattaattgg ggagcaaacc atgataactg aacagacaac agccattata attttacttt
      301 cattagcaat tggttagctc aattatagcc ccaaaaggta aattatcatc aacacataag
      361 caaaggactg acaggtgccg ccccccccac cagccgccca tttaccacaa ataaaaagcc
      421 ttcaggactg aatgcgtctg taacaaccgc actgatagtc tgccagaccc gccataacca
      481 gctgggtcag tattaactgg cagcgttcgc gtgaaaggta agtattctgc gcaatctccc
      541 cgactgtcgc cggttcggta acgcttaatt cattaaacac cactctggcg gtttctgtca
      601 tatcctgctg ttttagcatg tctttttccc ttttccggtt aacgtgacat accaataact
      661 cttgtcgaaa aagccagcaa gttgaaagac cggtattagc aaccaccagc gcgtttaacg
      721 ccccgtgccg tttttcagtc ataaaaaaac ccgcaaaaag cgggctcttt caaatgtcca
      781 tgtctgctat tcgcctcgcg gtacagcttt gcgaagctta ccggaattga agcagttttt
      841 acgtcaaaaa gcaataactt ttttctctat accaaaagcc ataaccattg gtttgtacaa
      901 aataaattct gccaccttta gccaatgctc aatgcgtctt tcacaggttc ttaaactcca
      961 ttccggatgt gcatcattca gcaattcagc cattttgcgc ttagtcatcc cccgccccac
     1021 ataacgctga ctcagaacat tgagcagccc gggataacct gccagtactt caccaataac
     1081 cctgtcgatt attaacgcct ctgaatcggt acaatgtgcc agccagcttt tttgattgcc
     1141 gttgatcata tcccgcaaaa aagcctcaag ttcaggtttg tccagacccg cttttttcat
     1201 cctccggagc gcctcgttaa ttgccgtttt tgtcagcttt ttagaggtca gtaattggtt
     1261 gaacatattt cccgtcttac cgtcgccaat atacgaccaa cgcccccaca tacgcagttt
     1321 cccctggatc cagacacttt ccagcgtttt caggcgtaaa tgctcaccgc ttttgcctgt
     1381 aatttccggg tatatcatat ttatgctcac tcactttcaa ttttgtaaat cttcacgccc
     1441 agccgcccac cagaaacgag ctggccgcgc acaatattga tttcatcaaa ctgctcgtcg
     1501 tctatgagaa gtccggcatg cgtcagcgca tccagtggtg ctttcaggat attgtccagg
     1561 tcacgacggc gcttatccgg tggctctgca ataatcttta tcgccagcct tccggacagg
     1621 tttaatttca gccgctgctg gcgaacaata agtgccacat cccggcgata acgctcaccg
     1681 gcttttgata caaaatatgt gctgccacga cgtcgccagt aggtgttcac cgtcggcggg
     1741 taaggcaaaa caaactctat acgcatcagt aacctctttt acccgagcac gccggttgca
     1801 aaggcgtgat caagaaaacg aaaaattaaa tcaacctggg aaccatgctt ttcttcgaac
     1861 gccagcggat ccgcatgaag ctcgttgtga tgctcccgac acagcggtag cgtgaaaata
     1921 tcgtgggatt ttgttcccat tcc
//
LOCUS       DAZFXT010000294         1910 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.297, whole genome shotgun sequence.
ACCESSION   DAZFXT010000294 DAZFXT010000000
VERSION     DAZFXT010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.297"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(59..355)
                     /locus_tag="ACHKW0_005140"
                     /pseudo
     CDS             complement(59..355)
                     /locus_tag="ACHKW0_005140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            417..1313
                     /locus_tag="ACHKW0_005141"
                     /pseudo
     CDS             417..1313
                     /locus_tag="ACHKW0_005141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
     gene            1264..>1910
                     /gene="traT"
                     /locus_tag="ACHKW0_005142"
     CDS             1264..>1910
                     /gene="traT"
                     /locus_tag="ACHKW0_005142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014695898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HFO7477774.1"
                     /translation="MSPRQPSGKNIIRWLLKKRTNGSVRYCQYTSTIWLEPASERTVF
                     LQIKNTSDKDMSGLQGKIADAVKARGYQIMTSPDKAYYWIQANVLKADKMDLRESQGW
                     LSRGYEGAAVGAALGGGITAYNTGSAGTTLGVGLATGLIGMAADAMVEDINYTMITDV
                     QIAERTRTSVRTDNVAALRQGTSGSKIQTSTETGNQHKYQTRVVSSANQVNLKFE"
BASE COUNT          529 a          463 c          481 g          437 t
ORIGIN      
        1 tggacaccac tttgtctaat tcgtcagatt ctgaccagac ggttcaggct gtacgcttac
       61 gattacgctg aacttcacgt gagatcgtcg aaggactgcg attcagcgca gtagctatcg
      121 cacgaatgct cattttggct gacaaaccag ctcgtatctc ctcgcgctca gacagtgtca
      181 ggtgagctac agcccgctta cgctcatggg gttttatgcc gccagtatcc cttaacatag
      241 tgaagatcgt tccgggtttt gaacccagga tattcgctat ttcactgaag cctgttccgt
      301 tcttccatag ttcaaaaaca gaggcttttt cctctgctgt aaatgttcgt ctcattcaaa
      361 aaacctccgc aaccccatgt tttcacataa ctgttgcgtt gaccaattga atctacaaca
      421 gcgtattttg cacaggagtc gctgaaaaat acgcgttaat cgaacaatgg cgacaacaat
      481 ttcccattga agcgatgtgt caggtatttg gtgtatccag gagcggttat tacaactggg
      541 tacagcatga accctcagac agaaaacaaa gtgatgagcg gctaaaactg gagattaagg
      601 tggcacatat ccgcactcgc gaaacatatg gaacccggcg gctccagacg gagctggcag
      661 agaatggcat catcgttggt cgtgaccgac tggcacgtct tcgtaaggag ctaaggctac
      721 gctgtaagca gaaacgcaag ttcagggcga ctacgaactc gaaccacaat ctgccagttg
      781 cgccaaatct gctgaaccag acgttcgctc ctacagcacc aaatcaggtc tgggtggcgg
      841 acctgacgta tgttgccaca caggagggat ggttgtacct cgctggcatc aaagatgttt
      901 atacgtgcga aattgtcggc tacgccatgg gagagcgcat gacaaaagag ctgacaggta
      961 aagccctgtt tatggcgctc aggagccagc gcccacctgc cgggctaatc caccactctg
     1021 atcgaggttc acagtactgc gcatacgatt accgggtcat acaggagcag tttggtctga
     1081 aaacatcaat gtcgcgtaaa ggtaactgtt acgacaacgc tccgatggaa agcttctggg
     1141 gaacgctgaa aaatgagagc ctgagccact atcgttttaa gagccgggat gaggccatct
     1201 cagtaatacg ggaatacatt gagattttct acaatcgtca gcgtcgtcac tctcgtctgg
     1261 ggaatatctc cccggcagcc ttcagggaaa aatatcatca gatggctgct taaaaaaaga
     1321 acaaatggta gtgtccgcta ttgtcagtac acctcaacca tctggcttga acctgcgagc
     1381 gaacgcaccg ttttcctgca gatcaaaaac acgtcggata aagacatgag tggactacag
     1441 ggaaaaattg ccgatgcagt caaagccaga ggttatcaga tcatgacttc cccggataaa
     1501 gcttactact ggatccaggc taacgttctc aaagcagaca aaatggatct gcgtgaatct
     1561 cagggatggc tgagtcgcgg ttatgaaggt gcagcagtcg gagctgctct tggaggtgga
     1621 atcacagcgt acaataccgg gtcggcaggt accactctcg gtgttggact ggctaccggt
     1681 ctgattggca tggccgcgga tgcaatggtg gaagatataa attacacaat gattacagat
     1741 gttcagattg ccgagcgaac cagaaccagc gtcagaacgg ataatgttgc agctttgcgt
     1801 cagggaacat caggctccaa aattcagaca agcacagaaa ccggcaacca gcataaatac
     1861 cagacccggg ttgtatccag tgccaaccag gttaatctga agtttgaaga
//
LOCUS       DAZFXT010000295         1907 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.298, whole genome shotgun sequence.
ACCESSION   DAZFXT010000295 DAZFXT010000000
VERSION     DAZFXT010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1907
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.298"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..141)
                     /locus_tag="ACHKW0_005143"
     CDS             complement(<1..141)
                     /locus_tag="ACHKW0_005143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7477775.1"
                     /translation="MELLLSLRIRSLIFLQKLIGRNNLTQRTRIKRLARRTIYFSRTV
                     VRN"
     gene            complement(323..>1907)
                     /gene="espL2"
                     /locus_tag="ACHKW0_005144"
     CDS             complement(323..>1907)
                     /gene="espL2"
                     /locus_tag="ACHKW0_005144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspL2"
                     /protein_id="HFO7477776.1"
                     /translation="LSKKRDASINLNGKVSDCNGEIIWCRHIASYWSEFFCSNSGKID
                     YETFSSPQLLSKAIVIQENKGTNNIKGDVFFVENESWGSVIYNLFLQLEKENKSHTSL
                     EVHSPGHAMALGIKIKNDKENKFVINFYDPNQTATHKRVFFCTNNICDIINLTAYDFL
                     SEQCLKCYGLKEDTLSLFVDKTKSNDNNNVFIKKLPDNILQGVVINFAMGAGLREIIK
                     KVYNDTRFTDLTKSQMKILCESKNVNNVPGLLLALQNGHDNVIDEYGTLIKKSNLNKE
                     ELIHILSARTLDGTIPGLYQALQNGHAQAIKSYGNLVLDTIDKNIDLEYLLSAFKYEA
                     HSSNKYTPGLFSAFQNGHADAIKAYCGVLGNSNLKRGEIIRMLEARNYDGAPGLLLAY
                     QNGDINTIQSFFDSLIMLDISKDFIEELLTAKHYDFTGLSLAISHRHDHVVKLYGKLF
                     KKLDTSPYKMSIILALAIDCERNNANIIIDSEYKSNKAVKEYVEILKEFNICPEKVAE
                     YLSEFSGKHFLDVYNYYSN"
BASE COUNT          590 a          351 c          277 g          689 t
ORIGIN      
        1 attacgtact acagtgcgtg agaaatagat ggttctgcgg gccagacgtt taatccgggt
       61 tctctgagtc agattgtttc gcccaatgag cttttgcagg aaaatgagtg atctgatcct
      121 taaactcagc aaaagctcga tttattcaac aaagcctgtc tgcgtttctc cggcccaaaa
      181 atatcaatca tctgttctaa aaaacagcat taagattatc acttatttaa gtaatattgg
      241 ttgtctgtag actcaggggg ctaaaataca tgtgcctgtg atactcaact gagagtgccg
      301 tgatagatac tttatatttt tttcaattgg aataataatt atatacatcg aggaaatgtt
      361 ttccgctgaa ctcgcttagg tattctgcta ccttttctgg gcatatattg aattctttta
      421 aaatttctac atattccttt actgccttat ttgacttgta ctcactatca ataattatgt
      481 ttgcattatt tctctcgcaa tcaattgcta atgctaggat aattgacatt ttatatggac
      541 tggtatcaag ttttttaaat aactttccgt ataatttgac aacatgatca tgtctatgac
      601 tgatcgctaa tgataacccc gtaaaatcat aatgttttgc agtcaataac tcttcgataa
      661 aatcctttga tatatctaac attattagtg agtcaaaaaa agactgaatc gtattaatat
      721 ctccattttg atacgcaaga agtaatcctg gtgcaccatc ataatttctc gcctcaagca
      781 tcctgattat ctcacctctt ttcaaattac tattgccaag tacgccacaa tatgccttta
      841 ttgcgtctgc atgaccattt tgaaatgcag aaaataatcc aggagtatat ttattagagc
      901 tatgtgcctc atatttaaat gctgaaagca aatactctaa atctatattt ttatcaatag
      961 tatccagaac taagttacca taactcttaa tagcttgagc atgtccattt tgaagggcct
     1021 gatataaacc aggtatggtt ccatccaaag ttcttgcaga taatatatgt attaattctt
     1081 ctttattaag atttgacttt ttaattaaag taccatattc atcaattaca ttatcatgtc
     1141 cattttgtag agctaaaagc aacccaggaa cattattaac attttttgat tcacacaaga
     1201 ttttcatctg agattttgta agatctgtaa atcgagtatc gttatatacc ttttttatta
     1261 tctctcttag tcctgctccc atagcaaaat taataacaac accttggaga atattatcag
     1321 gtaatttttt tataaacaca ttattgttgt cattcgattt tgttttatcc acaaaaagtg
     1381 acagtgtgtc ttcttttagc ccataacatt tcagacattg ctcactaaga aaatcatatg
     1441 ctgtcaggtt tattatatca caaatgttat tagtgcaaaa aaatactcgt ttatgcgtcg
     1501 cggtctgatt gggatcatag aaattaataa caaacttatt ttccttgtcg ttttttattt
     1561 taatgcccag agccattgca tgtccgggag aatgaacttc taatgatgtg tgagatttat
     1621 tctctttctc aagttgaaga aaaaggttat atataacgct tccccaagat tcattttcaa
     1681 caaaaaacac atcaccctta atattatttg tccccttgtt ttcctgaatt acaatagctt
     1741 tgcttaataa ttgaggggat gaaaatgttt cgtagtctat ctttcctgaa ttactacaaa
     1801 aaaactcact ccaatatgat gctatatgtc gacaccatat gatttcccca ttgcaatcac
     1861 taacctttcc atttagattt atactggcat ctctcttttt tgataaa
//
LOCUS       DAZFXT010000296         1895 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.299, whole genome shotgun sequence.
ACCESSION   DAZFXT010000296 DAZFXT010000000
VERSION     DAZFXT010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.299"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(42..86)
                     /locus_tag="ACHKW0_005145"
     CDS             complement(42..86)
                     /locus_tag="ACHKW0_005145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477777.1"
                     /translation="MFSKLAQSSIKAMF"
     gene            complement(322..432)
                     /locus_tag="ACHKW0_005146"
     CDS             complement(322..432)
                     /locus_tag="ACHKW0_005146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477778.1"
                     /translation="MGIKVQRLSLWMINTVLLLLPINNHHANTIKLIFEM"
     gene            complement(485..889)
                     /gene="ycgX"
                     /locus_tag="ACHKW0_005147"
     CDS             complement(485..889)
                     /gene="ycgX"
                     /locus_tag="ACHKW0_005147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HFO7477779.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYAIDNTLLYIEDVENNKPLI"
     gene            complement(1110..1841)
                     /gene="bluR"
                     /locus_tag="ACHKW0_005148"
     CDS             complement(1110..1841)
                     /gene="bluR"
                     /locus_tag="ACHKW0_005148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HFO7477780.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          533 a          393 c          390 g          579 t
ORIGIN      
        1 agctataatg ttatacattc aaatgactga acatcctgta attaaaacat agcctttatg
       61 ctactttgtg ccaatttgct aaacattatg gttgcctttt tatataacga taataatgaa
      121 tataagcatg acatgagaat aaggtttcaa tttttgagtt atataggaaa ggtttaacct
      181 gttcctggct aaaatacata taactggatg atgactaaac caaaacacat gtgcgttaag
      241 tattgaaacg ggcgtatggc acacccgttg tttttataaa tatattaacc gttataaaat
      301 aacgtataga aagtcaagtg atcacatttc aaatatcaat ttgatagtat tggcatggtg
      361 attatttatg ggtagcaata aaaggacagt atttatcatc catagggata gtctctgtac
      421 ttttattccc attatgctaa tgccttactg aattatgaag catttcttaa gcatccaact
      481 ttagctagat taatggttta ttattttcta catcttcaat atataaaagc gtattatcaa
      541 tggcgtagta actgcgtttg ttatgattaa catcagtaac ccaccggaaa acgcccgcgc
      601 ctgctagtgt tgaacagtat tcccgaaatg tagattttcc gcaaatatga agcaatgcgg
      661 cctcttttat tttagcaggg ttcttggtcg tactaacttt taacaggttc ctggttcctc
      721 ttaataacaa aaccgtgtca tcgtgagtaa taattctgat gttatccgta gccagataat
      781 aaatgtaatg tgcaatacgg tgatgtttta attctgaata aaaccaggag aagttttgct
      841 cttttctcac ttgctcaaac atcttttgaa aaacaacgac ctgatccatc aggataataa
      901 cctcttgtta gttgtgagac tgcgtagtgt gcacgatcgg ttttaccact tcaatctggt
      961 ctgtcctttg gctgtgatat gtacagagtg tgatagaggg aatatctgaa ttctcccggt
     1021 gagcattttg caacggacca gctccggtac aaacgctgtt gtgggttcag attataacat
     1081 tctgtctaag gggcgggata aaggtgaaat tagggggcat gaaagatgac tttataacct
     1141 tgctcacccc agtgttgtaa aagttcgttt tgccttctcg ttggtgccat gcctgtccag
     1201 acaatcaatg tttgcgtcgg gaacagttcc gggcgcggcg attcaatggg atcagcaaga
     1261 acagaaatgt gccatcctga caatgataaa cgccatgctt ccagccacaa tcgtgctctc
     1321 tcttcaacat tccacgccat cagaatggct tctttaccgg gcttacggcg catttcgaaa
     1381 agcgaggttg ctgcgtattc aattaatgcg ccgtcaaaca tactgctcat aatgcgggag
     1441 gtgttgtgat caagcacgag acgctggcga acaggaaggt aaacatgatt aatcaattga
     1501 tctactgggt actctcgacc cagtgaaata attctcgcgc gtagtttggc aggattagcc
     1561 atgcgaagaa ttgacatcat ctcttcttgc aggcggctcc agtcatcttc cgtatcctgg
     1621 ctggtggttt ccagtaatgc tttaactttg cctacaggga cgccattact tatccaacgc
     1681 ttgatctctt cgatgcgttg tatgtcttct tcatcaaaga gtcggtgtcc gccttcactg
     1741 cgctgtggtt ttaacaaacc gtagcggcgt tgccaggccc ggagagtgac aggattaatc
     1801 ccgcaacgtt cagcaacatc accaatgctg taataagcca caattcctcc ttgcggtcac
     1861 aaatctccgt cgcctgtaca cgacccaata atact
//
LOCUS       DAZFXT010000297         1859 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.300, whole genome shotgun sequence.
ACCESSION   DAZFXT010000297 DAZFXT010000000
VERSION     DAZFXT010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.300"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHKW0_005149"
     CDS             complement(<1..242)
                     /locus_tag="ACHKW0_005149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477781.1"
                     /translation="MAIKGLDQTIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETRVNRKLVKERSRLKRATVRNPNARIIVNRGDLP"
     gene            complement(257..610)
                     /locus_tag="ACHKW0_005150"
     CDS             complement(257..610)
                     /locus_tag="ACHKW0_005150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7477782.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGGGVRIEGSSPSLFVRTDTVRAVRRGDTLTINGEIFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(603..977)
                     /gene="gpFI"
                     /locus_tag="ACHKW0_005151"
     CDS             complement(603..977)
                     /gene="gpFI"
                     /locus_tag="ACHKW0_005151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7477783.1"
                     /translation="MATKEQNLKRLDELALILGREPDISGSAAEIAQRVAEWEEEIQS
                     SGEDVQVGDTVIRERETAAHDVREDTSGALTRIRVLTCLHLCGIDGETGESVEIADVG
                     RVILIMSSDAKTHVDGGMAVYA"
     gene            complement(1029..>1859)
                     /locus_tag="ACHKW0_005152"
     CDS             complement(1029..>1859)
                     /locus_tag="ACHKW0_005152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7477784.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEIQAVNAVLYGKYTMEGDQFEKIEVDFGRSTKNNITQGSGK
                     EWSKQDRDTFDPTHDLDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYIAEDGIEKRYLPEGMLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPDEFVVVQVK
                     "
BASE COUNT          429 a          587 c          414 g          429 t
ORIGIN      
        1 gggagatcac cgcggttaac gataattctg gcattcggat ttctgaccgt ggcccgtttc
       61 agtctggacc gttcctttac cagtttccgg ttcaccctgg tttcccgggc aacctgtgac
      121 gaagactgat taatcgccgt tgtggccacg cggttaatgg ccattgctga agccgccgga
      181 atggcgtttt tacgaacccg gctcagattt tcaatcgtct gatcaagccc ttttatcgcc
      241 ataattcacc ctgcgtttat cgtcgccggt taacggcggg tggttgacca cggttgagcc
      301 agagataaca gctgcccccg tcatccggag aaacacgatc cacccagaat atctcaccat
      361 taatggtcag cgtgtcacca cgccgcacgg cacgaaccgt atccgtccgc acaaataatg
      421 acgggctgct tccttcaata cggaccccgc caccggcaaa ccccagcgac tccggatcgt
      481 caaaaacccc ctgaacttcg ctgccacact gtgcccccga ggtgaactgc gcacagagcc
      541 ccatcacttc aacaatcgtg ctgtccaccc cggcaagggc agcatcaaag gcattctgaa
      601 aatcacgcat aaacagccat tccgccatca acgtgtgttt ttgcatctga ggacataatc
      661 agaatcaccc gaccaacatc cgcaatctca acggattccc ctgtttcacc atcaatgcca
      721 cagagatgga ggcaggtcag aactctgatg cgcgttaacg caccggatgt atcctcacga
      781 acatcatgag ccgcggtttc ccgctcccgg atcaccgtat ccccaacctg aacatcctcg
      841 ccggatgact gtatttcctc ttcccattcc gccacccgct gcgctatctc tgcggcactc
      901 ccggatatat ccggctcacg ccccagaatc agggccagtt catcaagccg tttcagattt
      961 tgctctttcg ttgccatatc agccccctgt gaaaaaagac acgggggcat ttcgcccccg
     1021 ctcacggatt atttcacctg taccaccaca aactcatccg ggtccggcaa caccatcagc
     1081 ggcgcggact gcgtcatggt aaattcacgg gcgggatccc ccaccgtcag ccagtgtttc
     1141 ggataacggg aagaggccac cacaccttcg gacaacgcct gcgcatcctg aatggcaccg
     1201 taacaacgga tcccatctgc agcagtattc cccagaacca gcatgccctc cggcagataa
     1261 cgtttttcga taccgtcttc tgctatataa gacgttttcg ccaccacaat ggccagatcg
     1321 ccgtaatacc ccttgaagga caccactgcg cccagatctt tcactgccgt ttcgagttga
     1381 gaatttgaac cgcgacgggt atccagtttt tcgcggaaca gcttaaaacc attcagaaga
     1441 cgccagacgg taccgtccat aatggcaata ttcacaagac cgctggcctg gtcgcagtag
     1501 aggtcaagat catgtgtagg atcgaacgtg tcacgatcct gttttgacca ctccttacca
     1561 ctaccctgag tgatgttatt cttcgtcgac ctgccaaaat cgacctcaat tttctcgaac
     1621 tggtctcctt ccatcgtata tttgccatac aacacagcat ttaccgcctg tatttcttcc
     1681 acctggacaa tcgcgtgctc ttcctgtttg aggttatcgg taatgatacg cagacggcgg
     1741 taagccggat cgttcagttg agccggatct tcaccaggaa ggcgctcaac cgcctgctgg
     1801 taattaaatt cgtgtttcgg cttgacgtag cccggacgta acacgcgggt ttcaccacc
//
LOCUS       DAZFXT010000298         1823 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.301, whole genome shotgun sequence.
ACCESSION   DAZFXT010000298 DAZFXT010000000
VERSION     DAZFXT010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.301"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(420..1358)
                     /gene="lrhA"
                     /locus_tag="ACHKW0_005153"
     CDS             complement(420..1358)
                     /gene="lrhA"
                     /locus_tag="ACHKW0_005153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HFO7477785.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPISAPEGDDSLLIERDIE"
BASE COUNT          500 a          423 c          403 g          497 t
ORIGIN      
        1 agcatttaat gccctttcac cccagatcct tgacgactcc aggataatta gatgttgttg
       61 aatcgtgtcc gttgtgaagc aatggaaaaa atacgggtct attttgacag gaatttgtgt
      121 cgattcctcc ccccaaaaga gagtattttc ttgatctgtg acacgctttt gtcattccat
      181 aacaaaaacg cagcaacaaa tttacgtatt ttttaacatc attgtagcag gtgatttttt
      241 tcaggcgatt atttgtgcgt acggggcgtg aatctctggt gattgaaaaa tgaacagttt
      301 tgtacgttct gcacgatgtg aagaaggttc tttactaaaa aaaagccgct ggggtttaaa
      361 acacccccag cggctcgttt tttacactat tgtctcagga attatctatc gtccgtcgat
      421 tactcgatat ccctttcaat caacaatgaa tcatcccctt ccggagcaga tattgggctg
      481 tattgccacg gattatggta gctctccatc gcctgataga tcacctgagc cagctcatta
      541 ttactggacg ggtcatagca cagcaaatat tcggtatcgg gtagcggcgg taagccatcg
      601 actccactca gcacacgcag gtctggactc atcatctcga ccggccttgc cgtcacgcca
      661 agaccggctt tcactgccgc acgaacggcc ggaagcgtcg aggcgacata agccagtcgc
      721 cagggaatat ctgctttatt aagcgtcgcc agcaccatat cgcgaaacgg acttggatca
      781 tccagcaata caagagggat cggctcacct ttttgcaata cgtattccgc tgcgcagtac
      841 cagtgtgttg gcgaggtacg caggttcaat gccttaaacg ctgaaggacg atgggtggtt
      901 accatcaaat ccacttcctg ggattccagc atttctgcca tataggcatt acgtttaacg
      961 cggacatcca gcgctaattt cggataaacc gaactcacgc gatttaacag gaaaggtaag
     1021 atcgtatcgg cagattcatc tgaagcaccg atagttaaca cgccctgaag attactgaac
     1081 attaatgatg agcaggcctc atcattaaaa cgcaggattt tcctggcgta accaagaagt
     1141 tgaatgccat gttcagttaa cagtttgttg cgaccgtgac gagcgaacag ttctttccca
     1201 acgagttgtt ccagacgctg catttgctga cttacggcgg actgagtacg acacacagcg
     1261 gcagctgcgg cagcaaaagt gttcagatcg gcaacagcaa caaatgttct cagcagatcg
     1321 aggtcgaggt taattatcgg acgatttgca cttatcatat attatcactt actggcggct
     1381 catactgagc tggttaatgc tgtgcacaca caaacaagca attccatttg taatgtgcct
     1441 ccctggcagt ttcatccgga acccggacga aagtaaaaat gcatatgagt tgcactaaaa
     1501 aagcgactca cattgttccg ttataatgcc tgaagtagat cacagaatat atcttcaggg
     1561 atcgcatatc tattaagtta ctcactcttt tctatttatg acatgcgcgt gtttgtataa
     1621 atgtaaatgt aagtccttgt tccactctcg tgcagcatcg ctggtcatac gcgaacacgt
     1681 acaaacagca atggtgaggc ggcatcaaga gcagggatcc gtcatttatg cgagcatttt
     1741 accccaaaaa ccttttattt ataagggtca ttgcgaatta tctgatgcaa agttatgtta
     1801 tgttaggcaa agtaatcttc tat
//
LOCUS       DAZFXT010000299         1754 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.302, whole genome shotgun sequence.
ACCESSION   DAZFXT010000299 DAZFXT010000000
VERSION     DAZFXT010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.302"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..795
                     /locus_tag="ACHKW0_005154"
     CDS             <1..795
                     /locus_tag="ACHKW0_005154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO7477786.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAE
                     YDEWGNLLNEENPHQLQQLIRLQGRYITQDPIGLKGGWNLYGYQLNPISDIDPLGLSM
                     WEDAKSGACTNGLCGTLSAMIGPDKFDSIDSTAYDALNKINSQSICEDKEFAGLICKD
                     NSGRYFSTAPNRGERKGSYPFNSPCPNGTEKVSAYHTHGADSHGEYWDEIFSGKDEKI
                     VKSKDNNIKSFYLGTPSGNFKAIDNHGKEITNRKGLPNVCRVHGNM"
     gene            767..1150
                     /gene="yhhH"
                     /locus_tag="ACHKW0_005155"
     CDS             767..1150
                     /gene="yhhH"
                     /locus_tag="ACHKW0_005155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417940.2"
                     /note="This BlastRule distinguishes YhhH from its paralog
                     YbbC. Both are named for founding members from Escherichia
                     coli K-12, and belong Pfam family PF15631; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhhH"
                     /protein_id="HFO7477787.1"
                     /translation="MSAEFMVICKKILFRNCVIVSLFVFTYNTWAQCNNNIKIMRKYE
                     SEGKYTVRNLVKNKAIALELAEIYVKNRYGQDAAEEEKPYEITELTTSWVVEGTIHSD
                     QIAGGVFIIEIGKNDGRILNFGHGK"
     gene            1255..>1754
                     /locus_tag="ACHKW0_005156"
     CDS             1255..>1754
                     /locus_tag="ACHKW0_005156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lyase"
                     /protein_id="HFO7477788.1"
                     /translation="MSNTYQKRKASKEYGLYNQCKKLNDDELFRLLDDHNSLKRISSA
                     RVLQLRGGQDAVRLAIEFCSDKNYIRRDIGAFILGQIKICKKCEDNVFNILNNMTLND
                     KSACVRATAIESTAQRCKKNPIYSPKIVEQSQITAFDKSTNVRRATAFAISVINDKAT
                     IPLLINL"
BASE COUNT          611 a          290 c          393 g          460 t
ORIGIN      
        1 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa aatccacctg
       61 taccactgcg accatcgcgg cctgccgctg gcgctcatca gcacggaagg ggcaacagcg
      121 tggtgcgcag aatatgatga atggggcaac ctgctgaatg aagagaaccc gcatcagctg
      181 cagcagctta tccggctgca ggggcgatat atcactcagg atccgattgg actgaagggg
      241 ggatggaacc tgtatggata tcaattgaat ccgatatcag acatcgaccc cctgggttta
      301 tctatgtggg aggatgcaaa atcgggggca tgtactaatg gtctttgcgg cacactatcc
      361 gctatgatag gtccagataa atttgattct atagatagca ccgcatatga cgccttaaat
      421 aaaataaata gccaatctat ttgcgaagat aaagagttcg ctggtttaat atgtaaggat
      481 aatagtggca gatatttctc aacagcacct aaccgaggag aaagaaaagg atcatatcca
      541 ttcaatagcc cttgccctaa tggtactgag aaagtatcag cttatcatac tcatggtgca
      601 gatagtcatg gagaatattg ggacgaaata ttttcaggta aagatgagaa aatagttaaa
      661 agtaaagata acaatatcaa gtcattttat ttaggtacgc ccagtggtaa ttttaaagca
      721 atagataacc acgggaagga aataacaaac agaaaaggat tacctaatgt ctgcagagtt
      781 catggtaata tgtaaaaaaa tattgtttag gaactgtgtc attgtatctt tgtttgtttt
      841 tacatacaac acatgggcgc agtgtaataa taatattaaa ataatgcgca agtatgaaag
      901 tgaaggtaaa tataccgtta gaaatttggt taaaaataaa gctatagcat tggaattagc
      961 tgagatatat gttaagaatc gttatggaca ggatgccgca gaagaagaaa aaccatacga
     1021 aatcactgag ttaacaacaa gttgggttgt tgaaggtacc attcactcag accaaattgc
     1081 tggtggggtt tttattatag aaataggcaa aaatgatggg agaattctga attttggcca
     1141 cggaaaataa gtatgtaagg ataatcaaac atcgtgtgcg ctgatggcag agtaggtgga
     1201 ggactccaga cagtcaaacg atagaaaaag atagccttta tggaggttcc tgcaatgtca
     1261 aatacatacc agaaaagaaa ggcaagtaaa gagtatggtt tatataatca atgtaagaaa
     1321 ctaaatgatg atgaattatt tcgcttactt gatgatcaca attccttgaa aaggatttca
     1381 tctgccagag tattacagtt aagaggtggg caagacgctg ttagattggc aattgagttc
     1441 tgctctgata aaaattatat ccgtagagat atcggagcat ttatactcgg gcaaataaaa
     1501 atttgcaaaa aatgcgaaga taatgttttt aatattttga acaatatgac attgaatgat
     1561 aagagcgctt gcgttcgagc tacggcaatc gagtcaacgg ctcagcgatg caagaaaaac
     1621 ccaatttatt cacctaaaat agtagaacaa tctcaaatta ctgcttttga taaatcgact
     1681 aatgtcagac gtgctacagc atttgctatt tctgttatca atgataaagc aacaattcca
     1741 ctattgatta atct
//
LOCUS       DAZFXT010000300         1716 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.303, whole genome shotgun sequence.
ACCESSION   DAZFXT010000300 DAZFXT010000000
VERSION     DAZFXT010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.303"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..631)
                     /locus_tag="ACHKW0_005157"
     CDS             complement(<1..631)
                     /locus_tag="ACHKW0_005157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7477789.1"
                     /translation="MTFKHYDVVRAASPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAITAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPDPRIKQLARRS
                     TVTPGGAACRYNDIIPADHCLHDVQDMSTLNHPKADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGAEGTFSADTGASQDSARWGVGKPLYQDL"
     gene            complement(1038..1205)
                     /locus_tag="ACHKW0_005158"
     CDS             complement(1038..1205)
                     /locus_tag="ACHKW0_005158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7477790.1"
                     /translation="MTVKLRLAVAALLLFLVVMVDFTSRIMSVLADGVLVCGIVVLLW
                     PVIKRNSLHNA"
     gene            complement(1202..1564)
                     /locus_tag="ACHKW0_005159"
                     /pseudo
     CDS             complement(1202..1564)
                     /locus_tag="ACHKW0_005159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448682.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
BASE COUNT          424 a          521 c          378 g          393 t
ORIGIN      
        1 tcaggtcctg atataacggt ttacccacac cccagcgcgc cgaatcctgg ctggcccccg
       61 tgtccgcact gaatgtcccc tccgcgccct gggtaaatgc cgaaccacca cgacagcatg
      121 gtaccagcag gatccccgcg ttattcggga tatacgggag cagttttttg gcaatatgta
      181 acccctggcc gacacagccg tactgccctt tgctcaggtc tgccttcgga tgattcagcg
      241 tactcatatc ctgcacatca tgcaggcagt ggtcggccgg aataatatcg ttatatctgc
      301 aggcagcccc acccggcgtc actgtactgc ggcgcgccag ctgtttaatg cgcggatccg
      361 gagcatcgta tgaatccggc agcggaagcc cttcaccgta agccatggca ttggactgcc
      421 cggccagtac gatgacgtag taccactccg gctcagttgc accactgacc accacatcac
      481 cttctgctgt aatcgcctgc atcagggtat aaggggttat ggccaccgga ctaccaaacg
      541 gctgccagcc ctctttcagt ttgtgtgtca gcttttccgc aaggtctgac ggcgacgccg
      601 ccctgacaac atcataatgt ttaaatgtca ttattcctcc cggccgggat agtgtattaa
      661 atcagatatg gagtgggctg tagtccggaa gcctgaatga cacacgggga ctacagccca
      721 agaaatgaaa aaaggccacg cagttgcgca gcctgataaa ccctggttaa aatccacacg
      781 ataacaacac aacaatatca gtatctcatg ctattgcccg aacccattcg ggcatttttt
      841 acccataaaa aagcccctcc ggagaggggc atgtttgcat gcacattctt tttcttgcat
      901 ggtgccgggt gcctcccggt gaattcagta tcagcacctg aatccgcgat tatcacatat
      961 acctggttgc tgattgcccc tccgcacagg gggattcacc atgcagtagt atttttaata
     1021 aacagtaaac aaaaaaatca agcattatgc aggctgtttc tttttatcac cggccacagc
     1081 aataccacaa tgccgcagac cagcacccca tccgccagca ccgacatgat tctgctggtg
     1141 aaatccacca tcaccaccag aaacagcagg agtgcagcca cagccaggcg cagttttacc
     1201 gtcactggtg attctccaga cgaagaccca gaacaccggc aatctcttcc agcaccttgc
     1261 gctcttccgg ctcaatttcg ccgtctgcct ccgcaatggc caccgccaca tccagcacat
     1321 cttccgcttc acgcgtatcg tgtttcacat cctcgatctc acgtaacgcc gcacgacgac
     1381 caattttaaa gttcgtatcc agctgaccga taatggttgc gctaatcgca ttaatttctg
     1441 acgtaaacgc ggacagcgca ggctgattac gcagtacctg ttcgatcttc gctttctagg
     1501 aagcctcaca ttcaccatct gcacaggcca ccaggtaggc agcattaata accgcctgtg
     1561 ccagatcgcg tttctcaaac tttccttttt ccggttaacg tgacacacca ataactcttg
     1621 tcgaaaaagc cagcaagctg aaagaccggt attcacaacc accagcgcgt ttactgtact
     1681 ggcgtgattt cagtcataaa aaaacccgcc tagcga
//
LOCUS       DAZFXT010000301         1706 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.304, whole genome shotgun sequence.
ACCESSION   DAZFXT010000301 DAZFXT010000000
VERSION     DAZFXT010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.304"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..518
                     /gene="nleG7"
                     /locus_tag="ACHKW0_005160"
     CDS             <1..518
                     /gene="nleG7"
                     /locus_tag="ACHKW0_005160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001121226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector NleG7"
                     /protein_id="HFO7477791.1"
                     /translation="IESVNGFTVNPVCSNHFSIFMARQNTIARNLEQQINNGRSFAQI
                     SQDFMLQLSSNIGWKKGAENALKNKIHSHSFVVNPDEFSCDTQFLKCPITLCVPEKGV
                     FVKNALNSNICTLYDKSAFMNLTREHLPHPLSREKIVKEMIIERNMCYFDTISQHFII
                     MDADQQKQHCK"
     gene            complement(743..1632)
                     /locus_tag="ACHKW0_005161"
                     /pseudo
     CDS             complement(743..1632)
                     /locus_tag="ACHKW0_005161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001299329.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein EspS"
BASE COUNT          506 a          346 c          325 g          529 t
ORIGIN      
        1 atatcgaatc agtcaatggt tttacagtaa atcctgtatg cagtaatcat ttctccatat
       61 ttatggcgag acaaaacact attgctcgca acctggaaca gcagatcaac aacggacgaa
      121 gttttgcaca aatatctcag gattttatgc ttcaattatc ttcaaatata ggatggaaaa
      181 aaggggccga aaacgccctt aaaaataaaa tccattctca ttcatttgtt gtaaatcctg
      241 atgaattctc ttgcgacaca caatttctta agtgcccaat aacattatgc gttccagaaa
      301 aaggggtttt tgtcaagaac gcactgaact ccaacatatg cactctttat gataagtctg
      361 cgttcatgaa tctcacaaga gaacatctac cccaccctct cagcagggaa aagatagtaa
      421 aagaaatgat tattgaaagg aatatgtgtt attttgacac cataagtcag catttcataa
      481 ttatggatgc agaccaacag aaacagcatt gtaaataaaa tgtaataatt acatactatt
      541 agtgattctc atgcatcgta agcggctcgc cagaaccgta ttgatattta ctgagcgctc
      601 agatcaactt tccatggcaa cagatcgcgt acccggtttg ccggccagtc ctggatatgc
      661 tcccgccgac aatattaatg gccaggaaat gtttaaaaca atgattctgt ttcctaacca
      721 tcaagtgtat atgctggctc aattactcaa tatctatggg ttgagttcta cccaaagtgg
      781 ctctgtagtc gcttcttctt acagtatgtt caggtgttgc aggcacagtt gtggagcgta
      841 gatgtgttgt tggtttacca tgctctggta cataaaaaac gccagtgcca tggtaacctg
      901 atatagaaac gttaattccg cgacgttcaa actcggcata gacgtgttct gctaaagaac
      961 tcttttgacc aaataatgcc ctggccaacc agccgttatt catatttgcg gatttctcta
     1021 tttcatcagg agagaagtct ttgtttttta atatgttggc tgagttacag gatgttaatc
     1081 tgatatcatc aatctcatga agattatgtt gaacaatgcg gtcgacaata tctgtcggcg
     1141 ataaaaattc atctccacac ttaagaagtg gtagaccagc agaaccatcc ccagacagat
     1201 aaattttttg tattttcctg gtgttaaatc atctggcagg atggttttca tctctgtcgc
     1261 tgtaatactt atagctgcag cagcaacaac atcgctatta tttgattgta gatggctttt
     1321 atttgctcca ggatatgtaa actccatttt ccttttatca aaatcttgtt ttgtagcatc
     1381 attcaggaat aataaatcat acggtttttt ccccgttgtg actcttgaaa cattttggcc
     1441 aagaatatta agagtatagt cattaacgga tttcattcct aaaaaaacaa gtaatccttc
     1501 tttctcttgc gctttttcga ttgttctccc tgctaaagtt attgggatgg attgagattg
     1561 ttttgtcgga actgatacgc tggcgctaat ataaagacgg cgcaatggcc gccacaggtt
     1621 ctttctgctc atccggcgtt atttctacag gtaataattc aacgccagtg tcagaagagg
     1681 ttgttaccgg aagacgccgc gaaaca
//
LOCUS       DAZFXT010000302         1701 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.305, whole genome shotgun sequence.
ACCESSION   DAZFXT010000302 DAZFXT010000000
VERSION     DAZFXT010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.305"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            5..>1701
                     /locus_tag="ACHKW0_005162"
     CDS             5..>1701
                     /locus_tag="ACHKW0_005162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HFO7477792.1"
                     /translation="MNISEQQLNNMMAAVSVALQPLVRVVPMTAVEWADQNYYLPKES
                     SYGEGEWKTLPFQIAIMNCMGNDQVRTVNLIKSARVGYTKMLLGVVGYFIEHKSRNSL
                     LFQPTDSAAEDFMKSHVEATIRNVPCLKDLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GTCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDESTPFGLKWEKDSPESVFYLCEH
                     HGCVIHQSELDQSNGRWICENTGMWTRDGLTFFSARGDEIPPPRSIMFHIWTAYSPFT
                     TWVQIVYDWLDALKDPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVRYTAAVPAR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL
                     "
BASE COUNT          406 a          400 c          495 g          400 t
ORIGIN      
        1 ctcgatgaat atatcagagc aacaactgaa taatatgatg gctgccgttt cggttgcgct
       61 gcagcctctg gtcagggttg taccaatgac ggcagttgaa tgggctgatc aaaattatta
      121 tctgcctaaa gaatcttcat atggtgaggg agaatggaaa acgctgccat tccagatcgc
      181 cattatgaac tgtatgggta acgaccaggt tcgcacggtt aacctgatta aatctgcccg
      241 tgttggctat acaaagatgt tgctgggggt ggtcgggtat tttattgagc ataaatcccg
      301 aaacagtctg ctttttcagc ccacggattc tgccgctgaa gattttatga agtctcacgt
      361 ggaggcgacg attcggaacg tgccatgcct gaaagacctt tccccatggc tgggtcgtaa
      421 acatcgtgac aatactctca cgctgaaacg cttttcatcg ggcgtcggtt tctggtgcct
      481 gggcggcgct gccgccaaaa actaccgtga aaaatccgtg gacgtggtct gctatgacga
      541 actttcctcg ttcgagccgg atgtcgaaaa agagggctcg ccaaccctgc tgggggataa
      601 gcgtattgag gggtcggtgt ggccaaaatc cattcgcggc tcgacgccta aaatcaaagg
      661 cacctgccag atcgaaaaag ccgctaacga gtcggcgcat tttatgcgtt tttatgtgcc
      721 ctgcccgcac tgtggggagg agcagtatct gaaatttggc gatgagtcca cgccttttgg
      781 gcttaaatgg gagaaggaca gccctgaaag tgttttctac ctctgtgaac atcatggctg
      841 cgtgatccat cagtctgagc ttgaccagag caacgggcgg tggatctgtg aaaacacggg
      901 catgtggacc cgtgacggtc tgacgttttt cagcgcccgg ggtgatgaaa ttccgccgcc
      961 gcgctccatc atgttccata tctggacggc gtacagtccg ttcaccacct gggtacagat
     1021 tgtctatgac tggctggatg cactgaaaga tcccaacggc ctgaaaacct ttgtgaacac
     1081 cacgctgggc gagacctggg aagaggccgt gggcgaaaaa ctcgatcacc aggtactgat
     1141 ggataaggtg gtgcgttaca cggcggcggt gcctgcccgg gtggtttatc tgacggcggg
     1201 cattgactcg cagcgaaacc gttttgagat gtatgtctgg ggatgggctc cgggagagga
     1261 agcctttctg gtggataaaa tcatcattat ggggcgtcct gatgaggaag agacgctgtt
     1321 acgtgtggat gcggcgatca acaaaaaata ccgccatgcg gatggcaccg aaatgactat
     1381 ttcccgtgtc tgctgggaca ccggggggat cgatggtgaa attgtttatc agagatcaaa
     1441 aaaacacggt gttttccggg tgctgccggt aaaaggcgca tctgtctatg gcaagccggt
     1501 gatcaccatg ccaaaaaccc gcaatcagcg gggcgtgtat ctgtgtgaag tgggaacgga
     1561 taccgcaaaa gaaattctct atgcccgtat gaaagccgat cccacgcctg cggatgaagc
     1621 cacgtcgtat gccatccgtt ttcctgatga tccggagatt ttttcgcaga cagaggcgca
     1681 gcaactggtg gcggaagagc t
//
LOCUS       DAZFXT010000303         1693 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.306, whole genome shotgun sequence.
ACCESSION   DAZFXT010000303 DAZFXT010000000
VERSION     DAZFXT010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.306"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..99
                     /locus_tag="ACHKW0_005163"
     CDS             <1..99
                     /locus_tag="ACHKW0_005163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7477793.1"
                     /translation="LARLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            116..376
                     /locus_tag="ACHKW0_005164"
                     /pseudo
     CDS             116..376
                     /locus_tag="ACHKW0_005164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390635.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
     gene            complement(518..1642)
                     /locus_tag="ACHKW0_005165"
     CDS             complement(518..1642)
                     /locus_tag="ACHKW0_005165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide disphospho-sugar-binding
                     domain-containing protein"
                     /protein_id="HFO7477794.1"
                     /translation="MRKRILFIGPPLYGLLYPLISLAQAFRVIGHDVVISSAGKFANK
                     AAEAGLVVFDAVPGLDSEAGYRHQEELRKKSNIIGHFSFFSDEMADNLIDFAGKWRPD
                     LIVYPPLDPAGPLVAAKYRIPSVMLAVGFAHTSAHIQMLNRSLSNAYRRHGVSGPLCD
                     LAWIDVAPPSMSILKNAGEPVISMRYIPYNGGAVKETWWDRDSDRKRLLISLGTVKPM
                     VDGLELISWVMDSANEVDADIILQLAINARTGLRKLPSNVRLVDWIPMGVFLNGADGF
                     IHHGGAGNTLTALYSGIPQIVFGEGADRSVNAEIVAKRGCGIIPDKHGLTSDLVNRLL
                     YDDSLRFCSDQVAAEMAEQPSPAEIAEVLMRKLKNNGKQL"
BASE COUNT          504 a          384 c          321 g          484 t
ORIGIN      
        1 ctggcaaggc tgggacggaa gtcactgtcg ttctcaaaat cggtggagct gcatgacaag
       61 gtcatcgggc attatctgaa cataaaacac tatcagtaag ttggagtcat tacctgagaa
      121 taatacctcc tgggtaagtg agcagatact ttcagcgcct ccgaaagatg taaatattcg
      181 acttggagtc ggttctttag aaggtacaac cgtctcacat gttcagcgat tgcatcattc
      241 gttaatcgca gctggtttgg aaagtaacct cactgtctat accggtggtc acgattatgc
      301 ctggtggcgc ggagcaatta ttgatgaatt agcaaattat aattgcagga aggtatcaaa
      361 taataactct gtgtaaaata tcgttttatc gtcagataac tctttctgca atattattat
      421 gggagactat ctcatttatt tagtgatgtc cttcatttat gtgatgaagg acattatatt
      481 actcataaca tttctcgttg aaaatttcag caatacgcta caattgtttc ccgttgtttt
      541 ttaattttct catcaaaacc tctgcaatct ctgcaggact gggttgttca gccatttcag
      601 cggctacctg atctgaacag aagcgtagtg aatcatcata aagaaggcga tttaccaaat
      661 cactggtcag tccatgcttg tccggaataa tcccacaccc acgcttcgca acaatttctg
      721 cattaacaga gcgatctgca ccttcgccaa acacaatctg tggtattcca ctatacagcg
      781 ctgtcagggt atttcctgcg ccaccgtgat gaataaatcc atcagctcca ttaaggaata
      841 cacccatagg tatccagtca accagacgta catttgatgg tagttttcgt aaaccagtac
      901 gagcatttat tgcaagttgc aaaatgatat cagcatcaac ttcatttgca gaatccataa
      961 cccatgaaat cagctccaga ccatcaacca ttggttttac agtgccaagg ctgataagta
     1021 aacgtttccg atcagaatcc ctgtcccacc atgtttcctt tacagcacct ccgttataag
     1081 gaatatatct cattgagata accggttctc cagcattttt aagaatgctc atacttgggg
     1141 gagcaacatc aatccatgct aaatcacata gtggaccgct gactccatgc cgcctgtaag
     1201 cattgcttaa agaacggttt aacatctgaa tatgggcaga tgtatgcgcg aatccaacag
     1261 ccagcatcac tgaaggaatt ctatatttag cagcaaccaa tgggcctgcc ggatcaagag
     1321 ggggatagac tattaaatct ggcctccatt ttcctgcaaa atcgatgagg ttatctgcca
     1381 tttcatcgct aaaaaaagag aaatgaccaa taatattact ttttttcctc aactcttcct
     1441 gatggcgata tccagcctct gaatctaaac ctggaactgc atcaaaaaca accagtccag
     1501 cttctgctgc tttattcgcg aatttgccag cgctactaat tactacatca tgtccaatta
     1561 cacgaaaggc ctgagccaga gaaatcaatg ggtataacaa accgtacagc ggtgggccaa
     1621 taaaaagaat acgttttcgc attaaaaaag tccgtcttct gcttacctgt ttatttttct
     1681 atctctaaga aaa
//
LOCUS       DAZFXT010000304         1683 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.307, whole genome shotgun sequence.
ACCESSION   DAZFXT010000304 DAZFXT010000000
VERSION     DAZFXT010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.307"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1223
                     /locus_tag="ACHKW0_005166"
     CDS             <1..1223
                     /locus_tag="ACHKW0_005166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7477795.1"
                     /translation="DKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKEKTLALGVYPE
                     VSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSH
                     SDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRY
                     AVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIDGYKGQPVTRLAVMLN
                     LLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSYRGSKMRTPHLVPLSQQA
                     VAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDTTQDVCGHGFRAMAC
                     SALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREQC
                     ISPFEYAKINNPLK"
     gene            complement(1409..1683)
                     /locus_tag="ACHKW0_005167"
                     /pseudo
     CDS             complement(1409..1683)
                     /locus_tag="ACHKW0_005167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          457 a          403 c          392 g          431 t
ORIGIN      
        1 ctgacaaggc ttataaactc actgacgggg ctggcatgtt cctgctggta catcctaatg
       61 gttcccgtta ctggcgtctc cgttatcgta ttctgggtaa agagaagact ctggcacttg
      121 gtgtgtatcc agaagtatct ctctccgaag ctcgtacaaa acgggatgag gcccgaaaac
      181 tgatttcgga gggggttgac ccttgcgaac agaaaagagc taaaaaagta gtccctgatt
      241 tacaactctc tttcgaacat attgcacgac gctggcatgc cagtaataaa caatgggcac
      301 aatcacacag cgataaagta ctcaaaagcc tcgagacaca cgtattcccc tttatcggca
      361 atcgggatat cacaacactc aataccccgg acctgcttat ccctgttcgt gctgcagaag
      421 ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag aatatctgct gtaatgcgtt
      481 atgccgtaca gtctggcatc atcagatata acccggctct ggatatggct ggtgcattga
      541 ccacggtaaa acgccagcat cgccctgctc tggatctttc acgcctgcct gaacttctgt
      601 cgcgtattga cggttataaa ggccagcctg tcacccgact ggcagttatg ctgaatttac
      661 tggtttttat tcgttccagt gaactcagat atgcccgctg gtctgaaatt gatattgaca
      721 atgccatgtg gactattcca gccgaacgcg aacctctgcc aggcgtaaaa ttttcatacc
      781 ggggctccaa gatgcgaaca ccacatcttg tgccactcag ccaacaggct gtagccatac
      841 tggcagaact tcagacatgg gcaggtgaaa atggtctgat ctttacggga gcacatgacc
      901 cacgtaaacc aatcagtgaa aacaccgtaa ataaagccct gagggttatg gggtatgaca
      961 caacccagga tgtctgtggt catggattcc gggcgatggc atgtagtgca ttaatagaat
     1021 caggtttgtg gtcccgcgat gctgtggaac gccagatgag ccatcaggag cgtaatggtg
     1081 tacgtgctgc gtatatccat aaagccgaac atctggaaga acgtcgactt atgctacagt
     1141 ggtgggcaga ttttctggat gcgaacagag aacaatgtat cagcccgttt gaatatgcaa
     1201 agattaacaa tccattaaaa tagtaatcat cccgggcagc tagcccggga attattttct
     1261 gaatattttt ctttgtgagt aactggaaaa aggatagcga agtaaattac tttaatgaat
     1321 tattccaggg gctcattaaa gttctttcgt aatactttgt agtcaaacac gttatggctg
     1381 gcatcaaaaa ccaggtaatg actccaactt attgatagtg ttttatgttc agataatgcc
     1441 cgatgacctt gtcatgcagc tccaccgatt ttgagaacga cagtgacttc cgtcccagcc
     1501 ttgccagatg ttgtctcaga ttatgtcgct caatgcgctg agtgtaacgc ttgctgataa
     1561 cgtgcagctt tcccttcagg cgtgattcat acagcggcca gccatccgtc atctatacca
     1621 cgacctcaaa ggccgacagc aggctcagaa tacgctccag tgtggccaga gtgcgttcac
     1681 cga
//
LOCUS       DAZFXT010000305         1621 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.308, whole genome shotgun sequence.
ACCESSION   DAZFXT010000305 DAZFXT010000000
VERSION     DAZFXT010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.308"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            256..825
                     /locus_tag="ACHKW0_005168"
     CDS             256..825
                     /locus_tag="ACHKW0_005168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HFO7477796.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRS"
     gene            1085..1486
                     /locus_tag="ACHKW0_005169"
     CDS             1085..1486
                     /locus_tag="ACHKW0_005169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7477797.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTELPWFEHTDDRLTEHGQQCPYVRPERREIQLIKRLQQFVLDALPVVRKASWHCRQC
                     HHDYYGERYCTHCQTGGFSIPRTTQEEICEF"
     gene            1474..>1621
                     /locus_tag="ACHKW0_005170"
     CDS             1474..>1621
                     /locus_tag="ACHKW0_005170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc ribbon protein"
                     /protein_id="HFO7477798.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDVLCCVSCGCPLTLQR
                     GNDGQ"
BASE COUNT          385 a          444 c          425 g          367 t
ORIGIN      
        1 gtattatgag taatcgcgtt tccacagtcc ctgcacttct ttttgcgtat caccatcact
       61 ccctcctcac agccttaccc gtaaccggtt ttttacatta aaaaaacaac gctgaaaatt
      121 caaccgtcag tccggagacg accgttcggg ttatcacaga gtgcctgaga cagtgtcctg
      181 ccggaggtca cttcagggac tctgcgcatt ttttacgacg tggctattcc gtatgaacca
      241 tacggagatt taaccatgac ctacaaatac aaccccttct ggcagcaacg tattcgtgag
      301 acggtgcggc acgcactgaa tgttcatccc cgcctgacgg cattgcgggt tgacctgcgt
      361 ttcccggatg taccggcagc aacggacgca gctgtgatat cccgcttcat caatgccctg
      421 aaagcccgaa tcgacgctta ccagaaacgt aagcatcggg aaggtaaacg cgtgcatccc
      481 acaaccctgc attacgtctg ggcccgggag tttggggagt gcaaaggtaa aaaacactat
      541 cacctgatgc tgctggttaa ccgggatacc tggtgtcgtg ccggtgatta ccgtgctccg
      601 ggatcactgg ccgggatgat taaacaggcg tggtgcagtg ccctgggagt ggatgtcggg
      661 tgccatgcca cgctggtgca ttttccggcc tggccggcgg tgtggctgga acgcgatgat
      721 gacactggct ttcagcaggt gctggaacgt gctgactatc tggcgaagga acataccaaa
      781 gctcactgca ccggtgagcg caattttggc tgtagccgga gttgagcacg actggcgcgc
      841 caatacccgg caaaccaaaa gtatgggttg ttgtctggcg gcaacgatgt atccactcgc
      901 ccactgactg ttttttacgt cagtgttcca ttgtgtgaca accacagtgc tgtaacagca
      961 accagcctgc tttacccaaa ctccagtcca gattttaact gaaccgccat tcacaccctg
     1021 accacgctgc ccgtaccggg acggctgttg cctgcgtgtc gtctggcggc tgaggaatat
     1081 gactatgtac gcaaaatcct ttatcgctct tgatggcaac ggacgtctga cgggcgcccg
     1141 tactgcacag gccgcacctt atgctaacta cacctgccac ttgtgtggca gtgcactcag
     1201 ataccatccg caatacgaca ctgaacttcc ctggtttgaa cacactgacg acaggctgac
     1261 agagcacggt caacagtgcc cttatgtcag gccggagcgc agagaaatac agttgattaa
     1321 acgtctgcag caattcgtac tggatgcctt acccgtggtg cgtaaagcca gctggcactg
     1381 cagacaatgt caccacgatt attatgggga gcggtactgc acacactgcc agaccggagg
     1441 attcagtatt ccccggacaa ctcaggagga aatatgcgaa ttctgaactg ttatatggcg
     1501 aatgacagca aaggccattt tgttacggcg aaagaagcag cgaagcacaa ccgccaggac
     1561 gttttgtgct gtgtgtcctg tggatgcccg ttaacacttc agcggggcaa tgacggacaa
     1621 c
//
LOCUS       DAZFXT010000306         1616 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.309, whole genome shotgun sequence.
ACCESSION   DAZFXT010000306 DAZFXT010000000
VERSION     DAZFXT010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.309"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1616
                     /locus_tag="ACHKW0_005171"
     CDS             <1..>1616
                     /locus_tag="ACHKW0_005171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO7477799.1"
                     /translation="DADKNIIKKILTEISKMPEDSIFTKINTRIPRDTMPILRRYHLW
                     PDGWNIRGLNGFMLSHKGSEVIDAVIAGQNQAYRELRRIRDNIHSEIYFKQTDELSSL
                     PDTDKIGGILVKKYLSGSLFSKFRQDTIIPEALSTLQISGPDLIQRKMLQFFRSRGVL
                     GEEFINERKLSDKAYIGVYKTTGTGKYDWLTPESIGVNDVTPADESTWCIGKGRCVDD
                     FLFKDVSTLKTENLPELFLTKIDTDTFFSQWSTKTKKDLQKKIQDLTVRYNELIDSST
                     IDFKNLYEIDQMLHMIMLEMNDDIAKRSLFSLQVQIAEKIRRMTIPVDNIINIYPDLH
                     KKNDNDLSMSIKGFLASNPHTKINILYSNKTEHNIFIKDLFSFAVMENELRDIINNMS
                     KDKTPENWEGRVMLQRYLELKMKDHLSLQSSQEANEFLEISTFIYENDFLREKIEAVK
                     NKMNSHELYFEKIKKEQNTWQDLSTKEQKLQLIKALKEISGNTEKDSHYDRLLDAFFK
                     KHNENIHNKIQRIKDEFKEYSRVAIHNIDK"
BASE COUNT          627 a          253 c          290 g          446 t
ORIGIN      
        1 gtgatgcaga taaaaacatt atcaagaaga tattaacaga aatatctaaa atgccagaag
       61 atagtatttt tactaagatc aatacaagga ttcctcgaga cacaatgccc atccttcgtc
      121 gttatcacct atggcctgat ggatggaata ttcgtgggct caatggattc atgctatcac
      181 ataaaggtag tgaagtgatt gatgctgtta tcgcaggcca gaatcaggct tacagggaac
      241 taagaagaat aagagataat attcatagtg aaatatactt caaacaaact gatgaattgt
      301 cctcacttcc agatacagac aaaattggag ggattctggt aaaaaaatac ctttcaggaa
      361 gtcttttttc aaaattcaga caagatacta ttatcccgga ggcattgagc accctccaga
      421 tatcaggtcc tgacttgatt caaagaaaga tgttgcaatt tttcaggagt agaggggtgt
      481 taggtgaaga gttcattaat gaaagaaaac tgagtgataa agcttatatt ggtgtctaca
      541 aaacaactgg cacagggaaa tatgactggt taacccctga atcgatcggc gttaatgatg
      601 tcacgcccgc agatgaaagt acctggtgta taggaaaagg ccggtgtgtt gatgacttcc
      661 tgttcaaaga tgtttcaaca ctaaaaacag aaaatcttcc agaattattc ttaacaaaaa
      721 tagatactga tacgtttttc tctcagtggt caaccaaaac caagaaagat ctgcaaaaaa
      781 aaatacaaga ccttactgta cgttataatg agctaattga ctcgtcgact atcgacttta
      841 aaaatctata tgaaatagat caaatgctcc atatgattat gctagagatg aatgatgata
      901 tagccaaaag gtcattgttt tcattgcaag ttcaaatagc cgaaaaaatt cggaggatga
      961 ccattcctgt agacaatata attaacatct atcctgatct acataaaaaa aatgacaatg
     1021 atctgagtat gtccataaaa ggctttcttg cgagtaatcc acatacaaaa ataaatattc
     1081 tttatagcaa taagactgag cataatattt ttataaagga tttattctcc ttcgcagtta
     1141 tggaaaatga gttaagagac attatcaata acatgagcaa agataagact cctgaaaact
     1201 gggaagggag ggtaatgtta caaagatatc tcgaattaaa aatgaaagat catcttagtt
     1261 tgcaatcttc tcaggaagca aatgagtttc ttgaaatatc tacttttatt tatgagaatg
     1321 atttcttgag agaaaagatt gaagcagtaa aaaacaaaat gaattctcac gaactttatt
     1381 ttgaaaaaat aaaaaaagaa caaaacacat ggcaggatct gtccacaaaa gaacaaaaat
     1441 tacagcttat taaagcattg aaagaaattt caggaaatac agagaaggac tctcattacg
     1501 atagacttct tgatgctttt tttaaaaaac ataatgaaaa tattcataat aaaatacaaa
     1561 gaataaaaga cgagttcaag gaatactccc gtgtagccat tcataatata gataaa
//
LOCUS       DAZFXT010000307         1594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.310, whole genome shotgun sequence.
ACCESSION   DAZFXT010000307 DAZFXT010000000
VERSION     DAZFXT010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.310"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>1594)
                     /locus_tag="ACHKW0_005172"
     CDS             complement(<1..>1594)
                     /locus_tag="ACHKW0_005172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7477800.1"
                     /translation="IEICDDDYAGISIGGRVLAVNSQTRTLTLDREITLPSSGTTLIS
                     LVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTLRQRLFRCVSIREND
                     DGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHLTAEVTADSGEYQVL
                     ARWDTPKVVKGVSFMLRLTVAADDGSERLVSTARTTETTYRFTQLALGNYRLTVRAAN
                     AWGQQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDPTVQFEFWFSEKRIT
                     DIRQVETTARYLGTGLYWIAASINIKPGHDYYFYIRSVNTVGKSAFVEAVGRASDDAE
                     GYLDFFKGKITESHLGKELLEKVDLTEDNASRLDEFSKEWKDANDKWNAMWGVKIEQT
                     KDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPANGNETPMFVAQGNQIFMNDV
                     FLKRLTAPTITSGGSPPVFSLTSDGKLTAKNADISGSVNANSGTLNNVTINENCQIKG
                     KLSANQIEGDIVKTVSKSFPRTS"
BASE COUNT          307 a          493 c          412 g          382 t
ORIGIN      
        1 tgctcgtgcg ggggaaagac ttgctgaccg ttttgacaat atcaccttca atctggttgg
       61 ctgacagttt ccccttaatc tgacagttct cattaatcgt gacgttgttg agcgtccctg
      121 agttcgcatt cacactgcca ctgatatccg catttttagc ggtcagcttt ccgtctgatg
      181 tcagggaaaa taccggaggg ctgccaccgc tggtaatggt gggggctgtc aggcgcttca
      241 ggaacacgtc gttcatgaat atctggttgc cctgcgccac aaacatcggc gtttcattcc
      301 cgtttgccgg gtcaataaac gcgatacggt tagcggcaac cagaaactgg ctcagcttgc
      361 cttcctccgt gtcctccatg ctgagaccaa tacccgcgac ataatgtttg ccgtctttgg
      421 tctgctcaat tttgacgccc cacatggcat tccatttatc gttagcgtcc ttccactctt
      481 tcgaaaactc atccagtctg ctggcgttat cctccgtcag atcaactttt tccagcagct
      541 ctttaccgag atgggattcg gttatcttgc ctttgaaaaa atccaggtaa ccttccgcat
      601 catcgctcgc ccgaccgacg gcctccacga atgccgattt gccaacggtg ttcacactgc
      661 ggatataaaa gtaataatca tggcccggtt tgatattgat actggcggct atccagtaca
      721 gccccgtgcc aaggtagcgg gctgtggttt caacctgcct gatatcggta atccgctttt
      781 ccgagaacca gaactcaaac tgtaccgtcg gatcataaac ggcaagatgc ggcgttgcgg
      841 ttatctgaaa atagcccggc gtcagctcaa tccgcgacgg tgctgccggt gcggcaatcc
      901 ggaacgatac cgacgccgga tcgccctgct gcccccaggc atttgccgcc cggactgtca
      961 gcctgtagtt ccccagcgcc agttgcgtga agcggtatgt ggtttccgtc gtccgggccg
     1021 tgctgaccag ccgctcactg ccgtcatccg ctgccacggt caggcgaagc ataaagctca
     1081 cgcccttcac caccttcggc gtgtcccagc gcgccagcac ctgatattcc ccgctgtctg
     1141 cggtgacttc ggcagtcagg tgctgcaccg cgggcggcgt gacgccattc accgtgccgc
     1201 tctggtcgcc gtcaaagtgc gccccgttat ccacgatggc ttctttttcg ggtacatgct
     1261 gcacggcggt gatggcatac gtgccgtcgt cgttctcacg gatactcacg cagcggaaca
     1321 ggcgctggcg caacgtcggc aacttcagcc cccatacgct gtattcagca acgccgtcag
     1381 gaacacggct cactttcacc ttcacgccgt cggtgacgga ctggacctcc acgctgaccg
     1441 gattaccctg cccgtcaacc aggcttatca gcgtggtgcc ggaggatggc agcgtgattt
     1501 cacggtcgag cgtcagcgtc cgggtctggc tgttcaccgc cagcacgcga ccaccgatgc
     1561 tgataccggc atagtcatca tcgcagattt caat
//
LOCUS       DAZFXT010000308         1576 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.311, whole genome shotgun sequence.
ACCESSION   DAZFXT010000308 DAZFXT010000000
VERSION     DAZFXT010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.311"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            82..852
                     /locus_tag="ACHKW0_005173"
     CDS             82..852
                     /locus_tag="ACHKW0_005173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7477801.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKINGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     AGKFGVSTRKVASTLAVVTATGRLARVNQNGKFRYCMPGDNLPAEPKAALVTESDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALRRA
                     KSDVQKWERVCAALRELNKHRDIVRQITDSSRRVVSEK"
     gene            868..1281
                     /locus_tag="ACHKW0_005174"
     CDS             868..1281
                     /locus_tag="ACHKW0_005174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7477802.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRRSGRETLRQLEAKTGATRYLMS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPVVVDPDLIWSLPDG
                     EIRRYDRQLNIICSECRNSEVMQRVLIFYTGVMME"
BASE COUNT          465 a          298 c          445 g          368 t
ORIGIN      
        1 gtgacgttta actgggatag ctggcgtcca aatgtcagca atatgagggt tgtgaagtaa
       61 ttttgtccgg aggaaatttt aatggaaacc gtatctgacg cactgaaagc actgaaaaaa
      121 gcctcttcac atgtggtggc agctcgcctt ggaatcagtc gtgaagaggc tgtcaacgag
      181 ctgtgggaac tcaaaataaa tggcgtcgtt gataaaactg gtcacacctg gtttctggct
      241 ggcgaaggtg aatcccgggt aaccgaagag cggccagtaa aatctgaagc acaggatatg
      301 ctgaccgggg aggtcgaaca aaaagttacc gcagacatga tgattgagtt tatcggtcag
      361 gatggggcta aaacgtgtga ggaactggcg ggtaagttcg gtgtcagtac tcgcaaggtt
      421 gcttccacgc tggcggtggt aaccgcaacg gggcggctgg cacgcgttaa tcagaacggt
      481 aaatttcgtt actgcatgcc gggcgataat ttaccagcag agccgaaagc cgcgctggta
      541 acggaaagtg atggtaaggc ctttcctcag ccagcaggtg ctgcgttacc agtccgggaa
      601 gccgcaacac aggaagaaat taaaacagaa actgtggcgg acattgtgca gccgttgcca
      661 tcgtttaccg aaacgcaagc agatgagctg atttttccgt cccttcgcag ggcaaacctg
      721 gcgctgcgca gggcgaaaag tgatgttcag aagtgggagc gagtctgcgc cgcgctgcgg
      781 gagctgaaca agcaccggga tattgttcga cagattactg attcttcccg ccgtgttgta
      841 tcggaaaagt gattgccgga ggcgcttatg gcaaaagtat ttacacagga agagcgggaa
      901 aaaattaaag ggcaggttgt tgagctagta cgccggagtg ggcgcgagac gttacggcaa
      961 ctggaagcca agacaggtgc gacaagatat ctgatgagcg ttcttgccag agagctggtt
     1021 gccagtggcg atgtatacaa ctctggttac gggttattcc catctgaaca ggctcgtaag
     1081 gactggcaaa acgcccgcaa aaaactatcg agggcaaaac tgaagaaacc ggttgtggtt
     1141 gatcctgacc ttatctggtc attaccagac ggagaaatac gccgctatga caggcaattg
     1201 aacataattt gtagtgagtg ccgtaacagt gaagtgatgc agcgagtttt gatattttac
     1261 acaggagtaa tgatggaata gtgaaaataa catgaaacct ttggattctg gtgtttcagt
     1321 ggatgggaaa agagcagata catagaaaat gaatagcaat aattcacagt ctggatgttg
     1381 tttgtatgct tataaaatga tcctagtatg cattgccgga gttgaatcag ctccaatgaa
     1441 gaaactgaga ggagtattta gaagatagct aaatggatgt aattattagt atctaaaaaa
     1501 tagtattttt gaaatgggtc taagaacaca acaatgtaca aatgagaagt tatcttttaa
     1561 tatgttcaaa gaccca
//
LOCUS       DAZFXT010000309         1569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.312, whole genome shotgun sequence.
ACCESSION   DAZFXT010000309 DAZFXT010000000
VERSION     DAZFXT010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.312"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..659)
                     /locus_tag="ACHKW0_005175"
     CDS             complement(<1..659)
                     /locus_tag="ACHKW0_005175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HFO7477803.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NG"
     gene            complement(823..918)
                     /gene="ymiC"
                     /locus_tag="ACHKW0_005176"
     CDS             complement(823..918)
                     /gene="ymiC"
                     /locus_tag="ACHKW0_005176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small membrane protein YmiC"
                     /protein_id="HFO7477804.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1032..1199)
                     /gene="yciX"
                     /locus_tag="ACHKW0_005177"
     CDS             complement(1032..1199)
                     /gene="yciX"
                     /locus_tag="ACHKW0_005177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YciX"
                     /protein_id="HFO7477805.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1202..1342)
                     /locus_tag="ACHKW0_005178"
     CDS             complement(1202..1342)
                     /locus_tag="ACHKW0_005178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588449.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HFO7477806.1"
                     /translation="MRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVVVALLIWKV
                     WG"
BASE COUNT          429 a          372 c          360 g          408 t
ORIGIN      
        1 ccgttgatca tagtggtgtg cgagtcagta ccaacgagtg tatccggata agcaatccat
       61 tcaccgtcct gcaattcact ccacactgct ttgccgagat attcgaggtt aacctgatgg
      121 caaatgcctg tgcctggcgg cacgacgcta aaccgactga acgcttgctt tccccatttc
      181 aggaacacat aacgttcgtg gttgcgctcc atttccaggc gtacgttttc ttcaaatgcc
      241 tcatcatcac caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc gaccggtgag
      301 agcgggttaa cctttgcagt atcgccgccg aggcgtttaa ccgcttcgcg cattgccgcc
      361 agatcaacaa cggcaggtac gccggtaaag tcctgcatca gcacccttgc cgggcggtag
      421 gcaatttcac ggtcagcatg ggcatttttc agccatcctg ccagcgcgtg gatatcctct
      481 tcggtaaccg agttaccatc ctgccagcgc agcaggtttt cgagcaaaac tttgagtgat
      541 ttgggcagac gggtgatatc gcccagtgat ttagcagcaa gcggcaggct gtagtagtgg
      601 taagttttat ctttggcctg caacgtgtcc ttactggctt ctcgtagggt tgacgacata
      661 gctcctcctt aatgacaggg ttgcgaagcc ctgattctct tcagggtgaa tattaaagat
      721 aacacaaaca tcgcgtaacg atttgataac aacccaaatt gataaaagag gagaaacctt
      781 gtggcaaagc aaatgacaac cccgccgcag cggggtcaag gatcagtgag taatgatcca
      841 gaggagttcg gcccagaaga gcatcgacag agaaaacgcg cccatccagg accaatattt
      901 catatttgtg ttgatcatag gctacaccct taacggttat tattttgtgt tgagataact
      961 caagctgtta ttgctgcggt gtattaaaag ccctttaatt cattaaaaat gggtaataag
     1021 aatgatgata attattgttt cggctgatca tcttcagcaa acagttctat aaaggcttgc
     1081 tgctgcgatg ttagttgcca gtcgtctgga ataacgacgg aacattcccg tttcgtttct
     1141 gtttcattgt cactatgctg gcataatggt gacgactcca gttgctcctg accgaccata
     1201 gttatcccca cactttccaa atcagcagtg cgacaaccac ccagaaaagt gcagaaccaa
     1261 gaaaaaccgc cagccaggct ttacgtttta attcaggatc gcggcggggt tcctgatttc
     1321 cagaaggcat tgctaacctc atataatcgc catcacttat cagcaagacg ttgaacgatt
     1381 ggtgtaatta atcatacgat gagacaaatc ctaaagaaac tttagtcaaa aatccagcga
     1441 atttacgaat cgtctccagt gaataattca atcttttgac ctgaaataga tatttgaaaa
     1501 ctggagtttg cgaggcgggt aattagacac tactttacag gtaatttacc ctataaatat
     1561 gagggataa
//
LOCUS       DAZFXT010000310         1569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.313, whole genome shotgun sequence.
ACCESSION   DAZFXT010000310 DAZFXT010000000
VERSION     DAZFXT010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.313"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1236
                     /locus_tag="ACHKW0_005179"
     CDS             <1..1236
                     /locus_tag="ACHKW0_005179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7477807.1"
                     /translation="YDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGNVAAVA
                     SAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGA
                     GTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSG
                     GAAGWVGEGKAKPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEA
                     VVARLDTDFVDPKKAAVADVSPASITHDVKGTASTGNPDADAEAAFGQFVAANLQPTG
                     AVWLMSSTNALALSMRKNALGQKEYPDMTLLGGSFQGLPVIVSQYVGDQLVLVNAPDI
                     YLADDGGVAVDMSREASLEMQSEPTGDSTTPSPVELVSMFQTGSVAIRAERWINWRRR
                     RTAAVAVITGVNYGSASGG"
     gene            1281..1502
                     /locus_tag="ACHKW0_005180"
     CDS             1281..1502
                     /locus_tag="ACHKW0_005180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477808.1"
                     /translation="MAKIRYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITVRRPA
                     GQKKKRDAEHGECGTFCGEPEKTRNQDVM"
     gene            1448..>1569
                     /locus_tag="ACHKW0_005181"
     CDS             1448..>1569
                     /locus_tag="ACHKW0_005181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7477809.1"
                     /translation="MWNLLRRTRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQG"
BASE COUNT          315 a          413 c          535 g          306 t
ORIGIN      
        1 tacgacaaca ccgcagcgga aatccgtcag gtggatgcgc acctgaagcg tctgcgtgaa
       61 ctggaagccg gtaaggccgc cacggcgcag ccggtgaaac aggccggtaa cgggaatgtg
      121 gccgcggtgg cttctgcgcc ggtgatccgt gtggagcaga aactggataa ggggattggc
      181 ttcgcccgct ttgccaaatc gctggctgcg gctaaaggcg tccgatctga agccctggaa
      241 gtggcccgtc gtcagtatcc ggatgacagt cgtctgcatc atgtcctgaa atcggcagtg
      301 ggcgcgggga ccaccacgga tccgcagtgg gcaggcagcc tgtctgaata tcaggaatac
      361 gcacaggact ttattgatta cctgcgtccg cagaccatta tcgggcgatt tggtcagggc
      421 gggatccctg cacttcgtca ggtgccattc aatatccgtg tgcacgccca ggtgtccggc
      481 ggtgctgccg gctgggtggg tgagggtaag gcaaaacccc tgacgaagtt tgattttgaa
      541 tccatcacct tcagtcatgc gaaggtgtcg gccattgcgg tactgacgga agaattgatc
      601 cgtttttcca gtccggctgc tgatgcactg gtccgtaatg cgctggcgga agcggtggtg
      661 gcgcgtctgg atacagactt tgtggacccg aaaaaagccg cagtggcaga tgtctccccg
      721 gcgtccatca cccatgatgt gaagggcacg gcatcaaccg gtaacccgga tgcggatgca
      781 gaggctgcgt ttggacagtt tgtggcagca aacctgcagc ccaccggtgc ggtctggctg
      841 atgtccagca ccaatgccct ggcactgtcc atgcgtaaaa atgcgctggg tcagaaggaa
      901 tacccggaca tgaccctgct gggtggctcc ttccaggggc tgccggtgat tgtctcccag
      961 tacgtgggtg accagctggt gctggtgaat gccccggata tttatctggc ggatgacggc
     1021 ggcgtggcag tggatatgtc ccgcgaggca tcactggaaa tgcagtctga gccgaccggc
     1081 gacagtacca cgccgtcccc ggtggagctg gtttccatgt tccagacagg cagcgtggcc
     1141 atccgtgcgg agcgctggat caactggcgt cgtcgccgta ccgcggcggt ggcggtgatc
     1201 accggagtga actacggcag tgcgtccggc ggctgagtct gatgaggagg acgggaggcg
     1261 tgcgcctccc gtaacaggtt atggcaaaga tccgatatct gcagggcacg catgatgccc
     1321 gggccgggga tatccgtgat gtggcacagc cgtgtgcgga ggtgctggtt cgcctgggga
     1381 aggcggagta catcacagtg cgacgtccgg caggtcagaa aaagaaacgt gatgcggagc
     1441 atggcgaatg tggaaccttt tgcggcgaac ccgaaaaaac cagaaatcag gacgtgatgt
     1501 aaaagaggtg ggctggacca gcctgtttca ggcggtggct gagccttttg ccggcgcctg
     1561 gcagcaggg
//
LOCUS       DAZFXT010000311         1569 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.314, whole genome shotgun sequence.
ACCESSION   DAZFXT010000311 DAZFXT010000000
VERSION     DAZFXT010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.314"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1236
                     /locus_tag="ACHKW0_005182"
     CDS             <1..1236
                     /locus_tag="ACHKW0_005182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HFO7477810.1"
                     /translation="YDNTAAEIRQVDAHLKRLRELEAGKAATAQPVKQAGNGDVVTVA
                     SAPVIRVEQKLDKGIGFARFAKSLAAAKGVRSEALEVARRQYPDDSRLHHVLKSAVGA
                     GTTTDPQWAGSLSEYQEYAQDFIDYLRPQTIIGRFGQGGIPALRQVPFNIRVHAQVSG
                     GAAGWVGEGKARPLTKFDFESITFSHAKVSAIAVLTEELIRFSSPAADALVRNALAEA
                     VVARLDTDFVDPKKAAVADVSPASITHDVKGTASSGNPDADAEAAFGQFVTANLQPTG
                     AVWLMSSTNALALSMRKNALGQKEYPDMTLLGGTFQGLPVIVSQYVGDQLVLVNAPDI
                     YLADDGGVAVDMSREASLEMQSEPTSDSSTPSPVELVSMFQTGSVAIRAERWINWRRR
                     RTAAVAVITGVNYGTASGG"
     gene            1281..1502
                     /locus_tag="ACHKW0_005183"
     CDS             1281..1502
                     /locus_tag="ACHKW0_005183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477811.1"
                     /translation="MAKIQYLQGTHDARAGDIRDVAQPCAEVLVRLGKAEYITARRPA
                     GQKKKRDAEHGECGTFCGDPEKTRNQDVM"
     gene            1448..>1569
                     /locus_tag="ACHKW0_005184"
     CDS             1448..>1569
                     /locus_tag="ACHKW0_005184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7477812.1"
                     /translation="MWNLLRRPRKNQKSGRDVKEVGWTSLFQAVAEPFAGAWQQG"
BASE COUNT          310 a          405 c          544 g          310 t
ORIGIN      
        1 tacgacaaca ccgcagcgga aatccgtcag gtggatgctc acctgaagcg cctgcgtgaa
       61 ctggaagccg gtaaggccgc cacggcacag ccggtgaaac aggctggtaa cggtgacgtg
      121 gtgacggtgg cctctgcgcc ggtgatccgt gtggagcaga aactggataa ggggattggt
      181 ttcgcacgtt ttgccaaatc gctggccgcg gctaaaggtg tccgctccga agccctggaa
      241 gtggcccgcc gtcagtatcc ggatgacagc cgtctgcatc atgttctgaa atcggcggtg
      301 ggggcgggga ccaccacaga tccacagtgg gcaggcagcc tgtctgaata ccaggaatat
      361 gcgcaggact ttattgatta cctgcgtccg cagaccatta tcgggcgatt tggtcagggg
      421 gggatcccgg cccttcgtca ggttccgttc aatatccgtg tgcatgccca ggtgtccggc
      481 ggagctgcag gctgggtggg tgagggcaag gccagaccgc tgacgaagtt tgattttgaa
      541 tccattacct tcagtcatgc gaaggtgtcg gccattgcgg tactgacgga agagctgatc
      601 cgtttttcca gcccggctgc tgatgcactg gtccgtaatg cgctggcaga ggcggtggtg
      661 gcacgtctgg acacagactt tgttgatccg aaaaaagcgg cggtggcgga tgtctcccct
      721 gcgtccatca cccatgatgt gaagggcacg gcgtcaagcg gtaatccgga tgcggatgcc
      781 gaggccgcgt ttggccagtt tgtgacggca aatctgcagc ccaccggtgc ggtctggctg
      841 atgtccagca cgaatgccct ggcgctgtcc atgcgtaaaa atgcgctggg gcagaaggaa
      901 tatccggaca tgaccctgct gggcgggacc ttccaggggc tgccggtgat tgtctcccag
      961 tatgtgggtg accagctggt actggtgaat gcgccggata tttatctggc tgatgacggt
     1021 ggtgtggcgg tggatatgtc ccgtgaagcg tcactggaga tgcagtctga accgaccagc
     1081 gacagcagca caccgtcacc ggtggagctg gtctccatgt tccagaccgg cagcgtggcc
     1141 atccgtgcgg agcgctggat caactggcgt cgtcgccgta ctgcggcggt ggcggtgatc
     1201 accggtgtga actacggaac tgcgtccggc ggctgagtct gatgaggagg gcgggaggcg
     1261 cgagcttccc gcagtaactg atggcaaaaa tccagtatct gcagggcacg catgatgccc
     1321 gggccgggga tatccgtgat gtggcacagc cgtgtgcgga ggtgctggtt cgcctgggga
     1381 aggcggagta catcacggcg cgacgtccgg caggtcagaa aaagaaacgt gatgcggagc
     1441 atggcgaatg tggaaccttt tgcggcgacc ccgaaaaaac cagaaatcag gacgtgatgt
     1501 aaaagaggtg ggctggacca gcctgtttca ggcggtggct gagccttttg ccggtgcctg
     1561 gcagcaggg
//
LOCUS       DAZFXT010000312         1568 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.315, whole genome shotgun sequence.
ACCESSION   DAZFXT010000312 DAZFXT010000000
VERSION     DAZFXT010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.315"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            107..1096
                     /gene="nleB"
                     /locus_tag="ACHKW0_005185"
     CDS             107..1096
                     /gene="nleB"
                     /locus_tag="ACHKW0_005185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB"
                     /protein_id="HFO7477813.1"
                     /translation="MLSSLNVLQSSFRGKTALSNSTLLQKVSFAGKEYPLEPIDEKTP
                     ILFQWFEARPERYEKGEVPILNTKEHPYLSNIINAAKIENERIIGVLVDGNFTYEQKK
                     EFLSLENEYQNIKIIYRADVDFSMYDKKLSDIYLENIHKQESYPASERDNYLLGLLRE
                     ELKNIPEGKDSLIESYAEKREHTWFDFFRNLAMLKAGTLFTETGKTGCHNISPCSGCI
                     YLDADMIITDKLGVLYAPDGIAVHVDCNDEIKSLENGAIVVNRSNHPALLAGLDIMKS
                     KVDAHPYYDGLGKGIKRHFNYSSLHDYNAFCDFIEFKHENIIPNTSMYTSSSW"
     gene            1145..>1568
                     /gene="nleE"
                     /locus_tag="ACHKW0_005186"
     CDS             1145..>1568
                     /gene="nleE"
                     /locus_tag="ACHKW0_005186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase NleE"
                     /protein_id="HFO7477814.1"
                     /translation="MINPVTNTQGVSPINTKYAEHVVKNIYPEIKHDYFNESPNIYDK
                     KYISGITRGVAELKQEEFVNEKARRFSYMKTMYSVCPEAFEPISRNEASTPEGSWLTV
                     ISGKRPMGQFSVDSLYNPDLHALCELPDICCKIFPKENN"
BASE COUNT          544 a          243 c          310 g          471 t
ORIGIN      
        1 aatgtcaaca atgctatgaa tttattttag aaaaacaatt aagctgacgt agtgttccgt
       61 ctaaatgacg gggcgatatt aacatgatta gaacaagagg aattttatgt tatcttcatt
      121 aaatgtcctt caatccagct tcagaggaaa gacagcctta tcaaatagta cacttctcca
      181 gaaagtttct tttgctggaa aagaatatcc tctggaacct attgatgaaa aaacccctat
      241 tctttttcag tggtttgaag caaggccaga gcgatacgaa aaaggagaag taccaatatt
      301 gaataccaaa gaacatccgt atttgagcaa tattataaat gctgcaaaaa tagaaaatga
      361 gcgtataatc ggtgtgctgg tagatggaaa ttttacttat gaacaaaaaa aggaatttct
      421 cagtcttgaa aatgaatatc aaaatataaa aataatctac cgagcagatg tggatttcag
      481 catgtatgat aaaaaactat ctgatattta ccttgaaaat atccataaac aagaatcata
      541 ccctgccagt gagagggata attatctgtt aggcttatta agagaagagt taaaaaatat
      601 cccagaaggt aaggactctt tgattgagtc atatgcagaa aaaagagaac atacttggtt
      661 tgattttttc aggaatttgg ccatgttgaa ggctggaact ttgtttacag agacgggaaa
      721 aactggatgc cataacatat cgccctgtag cggatgtata tatcttgatg ccgacatgat
      781 tattaccgat aaattaggag tcctgtatgc tcctgatggt atcgctgtgc atgtagattg
      841 taatgatgag ataaaaagtc ttgaaaatgg tgcgatagtt gtcaatcgta gtaatcatcc
      901 agcattactt gcaggcctcg atattatgaa gagtaaagtt gacgctcatc catattatga
      961 tggtctagga aagggtatca agcggcattt taactattca tcgttacacg attataatgc
     1021 tttttgtgat tttattgaat ttaagcatga aaatattata ccgaatacca gtatgtatac
     1081 cagcagttca tggtaagtct tccggttgtg gttgaaaaat atcgttcttt aggtatagat
     1141 tcagatgatt aatcctgtta ctaatactca gggcgtgtcc cctataaata ctaaatatgc
     1201 tgaacatgtg gtgaaaaata tttacccgga aattaaacat gattacttta atgaatcacc
     1261 caatatatat gataagaagt atatatccgg tataaccaga ggagtagctg aactaaaaca
     1321 ggaagaattt gttaacgaga aagccagacg gttttcttat atgaagacta tgtattctgt
     1381 atgtccagaa gcgtttgaac ctatttccag aaatgaagcc agtacaccgg aaggaagctg
     1441 gctaacagtt atatccggaa aacgcccaat ggggcagttt tctgtagata gtttatacaa
     1501 tcctgattta catgcattat gtgagcttcc ggacatttgt tgtaagatct tccctaaaga
     1561 aaataatg
//
LOCUS       DAZFXT010000313         1566 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.316, whole genome shotgun sequence.
ACCESSION   DAZFXT010000313 DAZFXT010000000
VERSION     DAZFXT010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.316"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1086
                     /locus_tag="ACHKW0_005187"
     CDS             <1..1086
                     /locus_tag="ACHKW0_005187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HFO7477815.1"
                     /translation="FVEVSGQPSNDGEGYLEFFREKIGKLHLAQGLWELIDNSQLADE
                     MAEMKTTITETRNEITQTVSKTLEDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKDG
                     IPYVAGIGAGIEDTDGQPLSNILLLADRIAMINPESGNSTPLFVAQGNQLFMNDVFLK
                     RLFAVSITSSGNPPAFSLTPEGKLTARNADISGAITANTGTLNNVTINENCVIRGKLS
                     ANQIEGDLVKTVGKAFPRNNSYASGTVTVTVYDDQGFDRQIIIPPVLFRGTKHQNFNS
                     PNQQSYWYSTCKLQVLKNGVEIFHEPATDVSRVFSSVIDMPAGRGHVTLTFNVSSAGA
                     NNWTPTTYISDLLVVVMKKSTAGISIS"
     gene            1155..>1566
                     /locus_tag="ACHKW0_005188"
     CDS             1155..>1566
                     /locus_tag="ACHKW0_005188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001408020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7477816.1"
                     /translation="MRHLYATILLFTTLLAGIAFPAQAESGHGAFSVGYAQVHPGGVP
                     ALSGTGARAGDLKGINVKYRYEFTDHLGGIVALSYASVKKSDTMKTGENTFHYESLRG
                     RYVSLMAGPVWQFSERVSLYGMAGMAYTRWSDSVQ"
BASE COUNT          399 a          367 c          459 g          341 t
ORIGIN      
        1 tttgtggaag tcagcgggca gcccagcaat gatggtgaag ggtatctgga atttttccgg
       61 gaaaaaatag gaaaactgca tctggctcag gggctatggg agctgataga caacagccag
      121 cttgcggatg agatggcgga gatgaagacc accatcaccg aaacccgcaa tgaaatcaca
      181 cagacggtca gtaaaacgct ggaagaccag agcgccacca ttcagcagat acagcgcgtg
      241 cagaaggaca caaatgatga cctggctgcg ctgtacatgc tgaaggttca aaaaacgaaa
      301 gacggcattc cctatgtggc cgggattggt gcagggattg aggatactga tggccagcca
      361 ctgagcaaca tactgctgct ggctgaccgt attgcgatga taaatccgga gagcggcaac
      421 agcacgccgt tatttgtggc gcaggggaat cagttgttca tgaacgatgt gttcctgaag
      481 cgactgtttg cggtgagtat cacctcgtcc ggcaatcccc cggcattttc cctgacgccg
      541 gaagggaagc tgacagccag gaacgcggat atcagtggag caattaccgc gaataccggc
      601 acgctcaata atgtcaccat taacgagaac tgtgtcatca gagggaaact gtctgcaaac
      661 cagatagaag gcgacctggt gaagacggtg gggaaagcct ttccccggaa taacagttat
      721 gccagcggga cggtaaccgt cacagtttac gatgaccagg gcttcgaccg gcagattatc
      781 attcccccgg tgctgtttcg cgggacgaaa caccagaatt tcaacagccc gaatcagcag
      841 tcgtactggt attccacctg taagctgcag gtgctgaaga acggggttga gattttccat
      901 gaaccggcaa cggatgtcag ccgggtgttc tcatcggtga tagatatgcc ggcagggcgg
      961 ggtcatgtca ccctgacgtt taatgtgtcg tcggccggtg cgaacaactg gacgccgaca
     1021 acgtacatca gtgatttact ggttgtggtc atgaaaaaat ccactgcagg gatcagtatc
     1081 agctgacggt ttattaaccc ggacgggcac ctcaggaggt gcctttttta ttgactgaaa
     1141 acaaagaggt aatcatgcgg catttatacg caacgatatt attgtttact accctgctgg
     1201 caggaattgc ctttcctgca caggctgaaa gcggacacgg tgcattttcc gtgggatatg
     1261 ctcaggttca cccgggcggc gtaccggcat tgtccggtac cggtgcgcgt gcaggtgatt
     1321 taaaagggat taacgtgaaa taccgttatg agttcacgga tcacctgggc ggcattgtcg
     1381 cgctgagtta tgcatcggtg aagaaaagtg acacgatgaa gacgggtgaa aataccttcc
     1441 attatgaaag cctgcgcggt cgttatgtca gtctgatggc cggccctgtc tggcagttca
     1501 gtgagcgggt cagtctctat ggcatggccg ggatggcgta cacccgctgg tctgacagtg
     1561 ttcagg
//
LOCUS       DAZFXT010000314         1563 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.317, whole genome shotgun sequence.
ACCESSION   DAZFXT010000314 DAZFXT010000000
VERSION     DAZFXT010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.317"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(204..857)
                     /gene="ribB"
                     /locus_tag="ACHKW0_005189"
     CDS             complement(204..857)
                     /gene="ribB"
                     /locus_tag="ACHKW0_005189"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HFO7477817.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(961..1109)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1231..1521
                     /gene="ubiK"
                     /locus_tag="ACHKW0_005190"
     CDS             1231..1521
                     /gene="ubiK"
                     /locus_tag="ACHKW0_005190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HFO7477818.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          443 a          397 c          354 g          369 t
ORIGIN      
        1 ctttttcgga tatattcata tggtcgcata ataacggaac aattatatga ttttgatcat
       61 aatgaaaaag aatgcctgat attaatgaaa ttttctcata gaaagagaag cggaattaac
      121 atcttgcata gcggggcaaa ataaaaaccg aagccagcta caacttcggt ttcttgatta
      181 aggcagtaat taagcagcgg ttttcagctg gctttacgct catgtgcctg acggtatgcc
      241 accaggtctt caatagtcac gagcgccata ttgtgtttat tggcaaactc aatgcactct
      301 ggtgcacgcg ccatcgtgcc atcgtcatta gtcagctcac acagcacacc agccggttta
      361 aagcctgcca gcgtcatcaa atcaatagtc gcttcagtat gaccgccacg cgtcagtaca
      421 ccacctgcct gcgcgcgaag tgggaaaacg tggcccgggc gattcagatc agaaggtttt
      481 gcgccatcgg caatcgctgc gcgaacggtc gtaatacgat cagcggcaga aacaccggta
      541 gtcacacctt cagccgcttc aatggtcacg gtaaaaccgg tgccataggc gctggtgtta
      601 ttttctacca tcattggcag atcaagttgt ttacggcgat cttcagtgat gcacaggcaa
      661 acaataccgc taccgtggcg aatggtcagc gccatctgct caacggtcat ggtttctgcc
      721 gggaagatca tatcgccttc gttttcacgg tcttcatcat caagcaccat tacaccgcgt
      781 ccttcacgca gcgcagccag tgcattttca acacgttcga aaggcgtacc aaaagaggaa
      841 agtagcgtct gattcatggt aaaaaaacct cactaaaatt atggttacca gaatcagggc
      901 agtcttagga gtggcggcat atagccaaaa taacgtgagc gggtccatgc ccgacagaat
      961 cgttactctc tcccatccgg actctaaccg tcggccccgg aattacaccg gatctgctgt
     1021 cctttgagtt cgcacccaaa gcgctcgcgg gctttcaact gagttgattt accgccggtg
     1081 gggaatttcg ccccgccctg agaataagcg gattcactat aacgctaatg attagcggca
     1141 gcaacgcata gcttcacata attctggttt atgacttacc cttatcgcac tacaatggta
     1201 ctcaacacct atcagtacag ggaaactaca atgattgacc cgaaaaaaat tgagcaaatc
     1261 gctcgccagg ttcacgaatc aatgcctaaa ggaatcaggg agttcgggga agatgtggag
     1321 aaaaaaatcc gccaaaccct acaagctcag ctgacgcgcc tcgaccttgt aagccgtgaa
     1381 gagttcgacg tccaaacgca agtcctgtta cggactcgtg agaaactggc gctgcttgaa
     1441 caaagaatga gcgaactgga aaatcgcagt actgagatta aaaagcagcc agatccagag
     1501 actctccccc caacgctgta aacatttgtc tttaatgcac tgtaaattat gaattaatcc
     1561 tct
//
LOCUS       DAZFXT010000315         1556 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.318, whole genome shotgun sequence.
ACCESSION   DAZFXT010000315 DAZFXT010000000
VERSION     DAZFXT010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.318"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..1386)
                     /locus_tag="ACHKW0_005191"
     CDS             complement(<1..1386)
                     /locus_tag="ACHKW0_005191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO7477819.1"
                     /translation="MATLRELIIKISANSQSFQSELQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELNSQLTEIRGSAVGMAGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIIRALAAGMGVARKDLK
                     AMADDGQLTADKVVPALISQLGVLRDEYAAMPETVSGSITKVENAFMAWVGGVNEASG
                     VTKTLSGVLNGVAGQIDNVATAVGALVAVGVARYFGNMASGAMSATAGLVTAARNEVA
                     LAEAQFRGTQIATARARAAVYRAQQAVAAARGTEMQIAAEARLAATQERLNRNIAARS
                     AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGTAAWYTLYQNQEQARESARQYALT
                     IDEIAHKTPSMS"
     gene            complement(1437..>1556)
                     /locus_tag="ACHKW0_005192"
     CDS             complement(1437..>1556)
                     /locus_tag="ACHKW0_005192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477820.1"
                     /translation="QGMNVSLEDAMLCWGEDEDKVESDPFAALEDALSFAAMS"
BASE COUNT          327 a          524 c          370 g          335 t
ORIGIN      
        1 agacattgac ggcgttttat gcgcgatttc atctatcgtc agtgcatact ggcgcgcaga
       61 ctccctggcc tgctcctgat tctggtacag cgtgtaccat gctgcagtcc ccagcatcac
      121 cagtccgggt acgccaccaa ccagccccag cgcaccgctc atcagacgtg agcccaccgc
      181 cgttgtactg ttcagcgcat tctgggcggc gcttctggca gcaatatttc tgttcaggcg
      241 ttcctgtgtg gccgccagac gggcctctgc agcaatctgc atctccgtcc cgcgggctgc
      301 cgccacggcc tgctgagcac ggtacacggc tgcccttgcc cgcgctgtgg caatctgcgt
      361 tcccctgaac tgtgcttccg ccagtgcaac ttcattacgt gcagccgtca caagtcctgc
      421 cgtggcagac atcgctccgg aggccatatt gccaaagtac cgggcaaccc cgacggcaac
      481 cagcgcaccc acagctgttg ccacattatc aatctgtccg gcaacaccgt tcagcacgcc
      541 ggagagcgtt tttgtcaccc cgctggcctc attcactccg cccacccagg ccataaaggc
      601 gttttccacc ttcgtgatac taccggaaac cgtttctggc atggcggcat attcatcacg
      661 caataccccc agctggctga ttaacgcagg aacgacttta tccgccgtca gttgaccatc
      721 gtccgccatc gccttcagat ctttacgggc cacgcccatg cctgcagcca gtgcacgaat
      781 gatccggtca ccactttcat tgaccgaatt aaattcctca ccgcgcaaca caccctgtgc
      841 cagcgcctgg ctgaactggg tgatcaccga acccgcctca gccgtactgg caccggagat
      901 tttcagcccc gtggaaatgg cctccgtcac cttcagcaca tcatcagcac tgtaaccata
      961 ttcacgcatc gaggcagccg aacgggcaaa cagggccgca ttatccgaaa aagcggtacc
     1021 tgtccgctgg ctgatatcca tcagcacttt ctgtgatgac gaaaattcat cagatgactg
     1081 cgacgcctgt ttcagacggg cattcacgga actccactca tcagccagtg aaatcaggtg
     1141 tccggtggca aaggcaccgg caaatgcgcc agccattccg acagcagaac cgcgaatttc
     1201 cgtcaactgg ctgttcagtt ctgccagggc acgtcgctgc tcccgggctg ccgcagcggc
     1261 ctgacgcccg ccattctgca gggtccggta atattcactg cccatacggg aagcacgctg
     1321 gagctccgac tggaatgact gtgaatttgc cgaaattttg ataatcagtt cacgtaacgt
     1381 cgccattcac ctttctccgg gcaaaaaaac ctgccacagc aggttttcat cattatttat
     1441 gacattgctg caaagctcag cgcgtcttcc agcgccgcaa acggatctga ctccacttta
     1501 tcctcatcct cgccccagca gagcatggcg tcctccagtg aaacattcat cccctg
//
LOCUS       DAZFXT010000316         1529 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.319, whole genome shotgun sequence.
ACCESSION   DAZFXT010000316 DAZFXT010000000
VERSION     DAZFXT010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.319"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1529
                     /locus_tag="ACHKW0_005193"
     CDS             <1..>1529
                     /locus_tag="ACHKW0_005193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7477821.1"
                     /translation="SSDGHYVYQFSEYGTDRVIDDNWHDGDVFTLNIANSTIDDDYEG
                     LYFTDSYLDGDVTKYTNETFHTPAGEGEEYAGLFANGGVGLGLAVNLDVESNINISNN
                     SRVAGISLTQGNTVNNTYTTESHTWDNNISVTDSTVTSGSVTTLEDSGFYGNSAEPSD
                     YSGNGEANDVALYFSDSAASNYSMKNNVYFSNSTLLGDVVFASTFNANFYPQGHDSNA
                     DGVLDTNGGWADDSLNVDELNITLDNGSKWVGSATTSANVDDESTASTDWYDVTGNSL
                     YPGVVTEANAWGRTIDDQVFQSGVFNVTLNNGSEWNTVNASNIDTLAVNNGSQVNVSE
                     SSLISDSIALTNGSSLNIGDEGYVGTDHLTVDSYSTVALSSDDNSSIYNGYYTPSLLA
                     NTITVTNGGLLDINVDQFDDNPFQTDTLELTSGNIADHNGNVVSGVFDIHSSDYVLNA
                     DLVNDRTWDTSKSNYGYGIVAMNSDGHLTINGNGDMNNGDELDNSSVDNVVAATGNYK
                     V"
BASE COUNT          419 a          298 c          346 g          466 t
ORIGIN      
        1 aagcagtgat ggtcattatg tctatcagtt ctctgaatac ggtactgatc gtgtaattga
       61 tgataattgg catgatggtg atgtattcac tttaaatatt gctaattcta caattgatga
      121 cgattatgaa ggtttatact tcactgattc atatctggac ggtgacgtta ccaaatatac
      181 taacgaaacc ttccataccc cagctggtga aggtgaagaa tacgccggtt tgtttgcaaa
      241 cggtggtgtt ggccttgggc tggcagtgaa tcttgatgtc gaaagtaaca tcaatatttc
      301 taacaactcc cgcgttgctg gtatcagcct gactcagggt aataccgtaa acaatactta
      361 cactactgaa tctcatactt gggataacaa tatctcagtt accgattcca cagtgacttc
      421 aggttctgta actactctgg aagacagtgg tttctatggt aactctgcgg aacctagtga
      481 ttactctggt aacggcgaag ctaatgatgt agctctgtat ttcagtgata gtgcggcttc
      541 aaattactcc atgaaaaaca atgtttattt cagcaattct actctgctgg gtgatgttgt
      601 tttcgcaagt actttcaacg caaacttcta tcctcaaggt catgacagta atgccgatgg
      661 cgtactggac accaatggtg gctgggctga tgactcactt aatgttgatg aactgaatat
      721 cactctggat aatggttcta agtgggtagg ctcagctact acatcagcaa atgttgatga
      781 tgaatctact gcttcgactg actggtatga tgttacaggt aatagcctgt atccgggcgt
      841 tgtaacagaa gcaaatgctt ggggtcgtac gattgatgat caagtattcc agagcggtgt
      901 gtttaacgtc actctgaata atggttctga atggaatacc gttaacgctt caaacattga
      961 tactttagct gtgaataatg gctctcaggt taatgtgtct gaatctagct tgatttctga
     1021 tagcatcgct ctgaccaatg gttcttcttt gaacattgga gatgaaggtt atgttggaac
     1081 tgatcatctg acagttgata gctacagtac tgtagcgctg tctagtgatg acaattcaag
     1141 tatctataat ggttattaca ctccgtcgct gttagcaaat acaataactg tgactaacgg
     1201 tggtttactg gatattaatg tagaccagtt cgatgataat ccgttccaga ctgatacttt
     1261 ggaactgacc agcggcaaca tcgctgacca taacggcaac gtagtgtctg gtgtattcga
     1321 tatccacagc agcgattatg ttctgaacgc tgatttggtg aacgaccgta cctgggatac
     1381 ttccaagtct aactacggct acggtattgt tgctatgaac tctgatggtc atctgactat
     1441 caacggtaac ggtgacatga acaacggcga cgaactggat aacagctctg ttgacaacgt
     1501 tgttgctgca accggtaact acaaagttc
//
LOCUS       DAZFXT010000317         1515 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.320, whole genome shotgun sequence.
ACCESSION   DAZFXT010000317 DAZFXT010000000
VERSION     DAZFXT010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.320"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1515
                     /locus_tag="ACHKW0_005194"
     CDS             <1..>1515
                     /locus_tag="ACHKW0_005194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477822.1"
                     /translation="ELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLE
                     TQTVDFTLGSQGLRHTPGDIIEICDNDYAGTMTGGRILSIDAASRTLTLDREVTLPET
                     GAATVNLINGSGKPVSVAITAHPAPDRIQVSTLPDGVATYGVWGLSLPSLRRRLFRCV
                     SIRENTDGTFAITAVQHVPEKEAIVDNGASFEPQSGTLNSVIPPAVQHLTVEVSAADG
                     QYLAQAKWDTPRVVKGVRFSLRLTSGSGEGSRLVTTAITADTEHRSSGLPLGEYTLTV
                     RAINSYGQQGEPATTTFRINAPAVPATIELTPGYFQITAVPRLAVYDPTVQFEFWFSE
                     TKIADTSQVETSARYLGTGSQWSVSGPHIKPGKDFWFYVRSVNLVGKSAFVEASGRAS
                     NDAEGYLGLFREKIGKLHLAQGLWELIDNSQLADEMAEMKTSITETRNEITQTVSKTL
                     EDQSATIQQIQRVQKDTNDDLAALYMLKVQKTKNGIPYVAGIGAGIEDTDGQPLS"
BASE COUNT          352 a          416 c          475 g          272 t
ORIGIN      
        1 gaactggtgg aagacccgga agccatactg cgctacgggc gcaacctgct gaagatggat
       61 gcgttcggtt gcaccagtcg cggtcaggcc caccgtgccg ggctgtgggt gataaagacc
      121 ggactgctgg aaacgcagac ggtggatttc acgctcgggt cacaggggct gcggcacaca
      181 cccggtgaca ttattgaaat ctgtgataac gactatgccg ggaccatgac cggcggacgt
      241 atcctgtcca tcgatgccgc cagccgcacc ctgacactgg accgtgaggt gaccctgccg
      301 gagacaggtg ccgccacggt gaacctgatt aacggcagcg gtaagccggt gagcgtggcc
      361 atcactgcac accccgcgcc ggaccggata caggtcagca ccctgcctga tggtgtggcg
      421 acatacggtg tatggggact ctccctgccg tcactgcgtc gtcgcctgtt ccgctgtgtc
      481 tccatccggg aaaacacgga cggcaccttt gccatcacgg cggtgcagca cgtaccggaa
      541 aaggaagcca tcgtggataa cggggccagc tttgagccgc agtcaggcac cctgaacagc
      601 gtcatccctc cggcagtgca gcacctgacg gtggaggtga gcgcagctga cggccagtat
      661 ctggcacagg cgaaatggga cacgccgcgg gtggtgaagg gggtgcgctt cagtctgcga
      721 ctgaccagcg gaagcggaga aggcagccgt ctggtgacca ccgccatcac cgcggataca
      781 gagcatcgtt ccagtggtct gccgctcggg gaatacaccc tgacagtcag ggcaattaac
      841 agttatggcc agcaggggga accggccacc accacgttca ggattaatgc acctgcggta
      901 cccgccacga ttgagctgac accgggctat tttcagataa cagcggtccc gcgtcttgcg
      961 gtgtatgacc cgacggtaca gtttgagttc tggttttcgg agacaaaaat cgcagacaca
     1021 tctcaggtgg aaacctctgc ccgttatctg gggaccggca gtcagtggag tgtatccggc
     1081 ccgcacatta agcccgggaa ggatttctgg ttttacgtgc gcagcgtcaa cctggtgggg
     1141 aaatctgctt ttgtggaagc cagtggccgg gccagcaatg atgcagaagg gtatctgggg
     1201 ctgtttcggg aaaaaatagg aaaactgcat ctggctcagg ggctgtggga gctgatagac
     1261 aacagccagc ttgcggatga gatggcggag atgaagacct ccatcaccga aacccgcaat
     1321 gaaatcacac agacggtcag taaaacgctg gaggaccaga gcgccaccat acagcagata
     1381 cagcgcgtgc agaaggacac aaatgatgac cttgctgcac tttacatgct gaaggtacag
     1441 aaaacaaaaa atggcatacc ctatgttgcc ggtattggag cggggattga ggatactgat
     1501 ggccagcccc tgagc
//
LOCUS       DAZFXT010000318         1433 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.321, whole genome shotgun sequence.
ACCESSION   DAZFXT010000318 DAZFXT010000000
VERSION     DAZFXT010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1433
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.321"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..321
                     /locus_tag="ACHKW0_005195"
     CDS             <1..321
                     /locus_tag="ACHKW0_005195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2511 domain-containing protein"
                     /protein_id="HFO7477823.1"
                     /translation="VTFLSMFSFSAVAMPFKTIERESFNGVWPFNSDEVQLQCLDGNP
                     YVMNFDDNKLYALTGLARIKGKTFGALPLDNNNPFWLDNDAAPGLKKSLGDVTKAAFD
                     LCDK"
     gene            327..1328
                     /locus_tag="ACHKW0_005196"
     CDS             327..1328
                     /locus_tag="ACHKW0_005196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7477824.1"
                     /translation="MSVRKIPSGKWLCECYPYGASGKRIRKQFATKSEALSYERRLMN
                     SRVGDEFQDGSGPRLSELIARWFEMYGKTLSSGAERKVKLEAICSRLGDPFASQFDKN
                     MFATYRERRLSGEWNPKGKKKLSEATVNREQSYLHAVFAELKRLGEWSGENPLTGIRK
                     FREEEKELAFLYVDEIERLLIACDESRNKDLGVVVRIGLATGARWSEAEGLKQSQVLP
                     GRITFVKTKGKKNRTVPISPQLQAMLPKKRGALFSPCYEAFDAAIKRAKIELPDGQLT
                     HVLRHTFASHFMMRGGNILVLQKILGHSDIKMTMRYAHFAPGHLEAAVELNPFDNRG"
BASE COUNT          405 a          241 c          359 g          428 t
ORIGIN      
        1 gttacttttt tatctatgtt ttctttttcc gccgtcgcaa tgccttttaa aactattgaa
       61 cgtgagagtt tcaatggggt atggccattt aattctgatg aggttcaatt acagtgtctt
      121 gatggtaatc cttatgtgat gaattttgac gataataagt tatatgcact tacaggtttg
      181 gctcgaataa aaggtaaaac atttggtgcg ttaccgttag ataacaataa tccattttgg
      241 ctagataatg atgccgcccc agggttaaaa aagagtctgg gggatgtcac taaggctgca
      301 tttgatttat gtgataagta actaaaatgt cggttcgtaa gattccatca ggtaaatggc
      361 tttgcgaatg ttatccctac ggggcatcgg gaaaacgcat tcgtaaacag tttgcgacaa
      421 aaagtgaggc gctctcttat gagcgccgtt taatgaatag tagagttgga gacgagtttc
      481 aagatggttc tggtcctcgt ctttctgagt tgattgctcg ttggtttgag atgtacggta
      541 aaaccttgtc ctctggtgca gagcgcaaag tcaaacttga ggcgatttgt tccaggctgg
      601 gagatccatt tgcttctcag tttgacaaaa atatgtttgc cacttatcgg gaaagaaggc
      661 tatcaggaga atggaatccc aaggggaaga aaaaacttag tgaagcaacc gttaatcgcg
      721 agcagtcata tctacatgct gtttttgccg aactgaagcg ccttggggag tggtctggtg
      781 aaaaccccct gactggtatt cgcaagtttc gtgaggaaga aaaggaactg gcgtttctgt
      841 atgtagatga gattgaacgc cttctgattg cgtgtgatga gtcacggaat aaagatttgg
      901 gggttgttgt ccgtattggg cttgcgactg gtgctcggtg gagtgaagca gaaggattaa
      961 agcaatctca agtactgccc ggtcgaatca catttgttaa aactaaagga aagaagaacc
     1021 gcactgtacc gatttcacct caattgcagg ctatgcttcc caaaaaacga ggagcgctat
     1081 tttcaccatg ttatgaggct tttgacgctg caattaagag agcgaagatc gagcttcctg
     1141 atgggcaatt aactcatgtg ctacgtcaca cgtttgccag tcattttatg atgcggggcg
     1201 gaaatattct tgtgttgcaa aaaatactgg ggcatagcga tataaaaatg actatgcgtt
     1261 atgcgcattt tgctccaggt catttagagg ctgctgttga attgaaccct tttgacaata
     1321 gagggtaaaa agtggcgata aaaagtgaca atagattcaa atggcgttgt atagagccat
     1381 tttcaaagtt aataaaatca taatgttatg atttataaat ggtggttgat agt
//
LOCUS       DAZFXT010000319         1424 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.322, whole genome shotgun sequence.
ACCESSION   DAZFXT010000319 DAZFXT010000000
VERSION     DAZFXT010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.322"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            119..466
                     /gene="tnpB"
                     /locus_tag="ACHKW0_005197"
     CDS             119..466
                     /gene="tnpB"
                     /locus_tag="ACHKW0_005197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7477825.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            516..>1424
                     /locus_tag="ACHKW0_005198"
     CDS             516..>1424
                     /locus_tag="ACHKW0_005198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc8 family transposase"
                     /protein_id="HFO7477826.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSLSYLGEDTAEQLELMRSAFRVIRTVREKHACTQC
                     DAIVQTPAPSRPIERGIAGPGLLARVLTSKYAEHTPLYRQSEIYGRQGVELRRSLLSG
                     WVDACCRLLSPLEEALHGYVMTDGKLHADDTPVQVLLPGNKKTGRLWAYVRDDRNAGS
                     ALAPAVWFAYSPDRKGIHPQTHLACFS"
BASE COUNT          308 a          408 c          424 g          284 t
ORIGIN      
        1 cagaggaccc ggaaaccctc agtatcagct gtgaggtaac gttccggcac gggacgctcc
       61 gcttcaatgg caatgtcagc gaaaagctcc tgactctgct gatacaggaa ctgaagcgat
      121 gatcccgtta ccttccggga ccaaaatttg gctggttgcc ggtatcaccg atatgagaaa
      181 tggcttcaac ggcctggctg cgaaagtaca aacggcgctg aaagacgatc ccatgtccgg
      241 ccatgttttc attttccggg gccgcagcgg cagtcaggtt aaactgctgt ggtccaccgg
      301 tgacggactg tgcctcctga ccaaacggct ggagcgtggg cgcttcgcct ggccgtcagc
      361 ccgtgatggc aaagtgttcc ttacgcaggc gcagctggcg atgctgctgg aaggtatcga
      421 ctggcgacag cctaagcggc tgctgacctc cctgaccatg ctgtaaatct ctttatcctg
      481 gttgtcacag aataagcccg gtaaaatacg ggcttatgaa cgacatctct tctgacgaca
      541 tcttcctgct gaaacagcgc ctggccgaac aggaagcgct gatccacgcc ctgcaggaaa
      601 agctgagcaa ccgggagcgc gaaatagacc atctgcaggc gcagctggat aaactccgcc
      661 ggatgaactt cggcagtcgt tccgaaaaag tctcccgccg tatcgcacaa atggaagccg
      721 atctgaaccg gcttcagaaa gagagcgata cgctgactgg tagggtgtat gacccggcag
      781 tacagcgtcc gttgcgtcag acccgcaccc gtaagccgtt ccctgaatca ctaccccgtg
      841 acgaaaagcg actgttgcct gcggcgccgt gctgcccgaa ctgcggcggt tcactgagct
      901 atctgggcga ggataccgcc gaacagctgg agttgatgcg tagcgcattc cgggttatcc
      961 ggacggtacg ggaaaaacat gcctgtactc agtgcgatgc catcgtgcag acacctgcac
     1021 cttcgcggcc catcgagcgg ggtatcgccg gaccggggct gctggcccgc gtgctgacct
     1081 cgaagtatgc agagcacacc ccgctgtatc gccagtcaga aatatacggc cggcaaggtg
     1141 tggagctgag gcgttcactg ctgtcgggct gggtggatgc atgctgccgg ctgctgtctc
     1201 cgctggaaga ggcgcttcat ggctatgtca tgactgacgg caaactccat gccgatgata
     1261 ccccggtcca ggtactgctg ccgggtaata agaagaccgg gcggttgtgg gcgtatgttc
     1321 gtgatgaccg caatgcaggg tcagcgttgg cacctgcagt gtggttcgct tacagcccgg
     1381 acagaaaagg catccatccg cagactcatc ttgcctgctt cagc
//
LOCUS       DAZFXT010000320         1421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.323, whole genome shotgun sequence.
ACCESSION   DAZFXT010000320 DAZFXT010000000
VERSION     DAZFXT010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.323"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(71..283)
                     /locus_tag="ACHKW0_005199"
                     /pseudo
     CDS             complement(71..283)
                     /locus_tag="ACHKW0_005199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309678.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            433..1101
                     /locus_tag="ACHKW0_005200"
     CDS             433..1101
                     /locus_tag="ACHKW0_005200"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HFO7477827.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          430 a          318 c          255 g          418 t
ORIGIN      
        1 gccgaaaact gatattactt taatcaaaag ttgacgtgcc agattcgaag taaatttata
       61 aaatatcaaa tcaatataca ttttgcacct tcgagatagt aatgactgtt actgtaacat
      121 gagttacaaa cagcttcata cccagagcta ctgatgtatc aactcgattc aacaataccc
      181 gatatttatt ccatgcctcc agcaacgatc tttcttcctc cgttgcgatt tccagatcta
      241 cagcatcctg cagtggcgca atatactcac tgaattcctg gatgtagaac tgtgtggtga
      301 cggtcttcca gccattcagc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc
      361 tatgctgcgg cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa
      421 taaaactata acatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac
      481 acaccactta ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca
      541 tggggatgtc ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac
      601 catctggata ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg
      661 atatctttaa tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa
      721 tcaaaaatta aacataaaat taaccatgat gtttttgaac cttatcccgc ggcgttacat
      781 ggtcaatttg attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct
      841 acaaaaagct gtgtaatacg caatgcggcg caggccttaa ctgacgatgg aactctatac
      901 ggagccacaa ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc
      961 atttacaatc agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat
     1021 atactctcag agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt
     1081 tccgcttcag ggaaaaaata gcatccaacc gcagcacgtt cttgcataag gcgtgtctgc
     1141 ggtttttcaa ctattcagat acatcactcc catcacattc attcctccgc atcataggca
     1201 tataggctat atcaccttga tatttttctt cttcagataa aaactgttat ctatgtatac
     1261 ttttaaaccc aatccgtgta gagtctctgc ataagatagt ttgcagttgc cacttcagct
     1321 tgcgccataa accgcctgat ttttgccgcc acctgttagc attcctgtat acctgaaacg
     1381 acaatgttta tctacgaact ttaagaacac ccaggataaa a
//
LOCUS       DAZFXT010000321         1389 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.324, whole genome shotgun sequence.
ACCESSION   DAZFXT010000321 DAZFXT010000000
VERSION     DAZFXT010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1389
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.324"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..102
                     /locus_tag="ACHKW0_005201"
     CDS             <1..102
                     /locus_tag="ACHKW0_005201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 C-terminal
                     domain-containing protein"
                     /protein_id="HFO7477828.1"
                     /translation="KKHEYKEKYKHLLYQKKFLDGVFTAIKYNVMKG"
     gene            111..1253
                     /locus_tag="ACHKW0_005202"
     CDS             111..1253
                     /locus_tag="ACHKW0_005202"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HFO7477829.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKRAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLEKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMHCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFEHYSWDGVTQRFEEVIHNWFE"
     gene            complement(1289..>1389)
                     /locus_tag="ACHKW0_005203"
     CDS             complement(1289..>1389)
                     /locus_tag="ACHKW0_005203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477830.1"
                     /translation="FIGYFVIRGNVEQIEPNLLGVYAGLLLAMKNK"
BASE COUNT          463 a          227 c          269 g          430 t
ORIGIN      
        1 aaaaaacatg agtacaaaga aaaatataaa cacttgcttt accagaaaaa atttctcgat
       61 ggtgttttta cagcaattaa atataatgtt atgaaaggtt aatatacgag atggttgata
      121 aaataatatt tacggttact cctatttttt caattcctcc tcgcggagcg gctgctgttg
      181 aaacgtggat gtatcaggtt gctcagcgta ccaattttcc aaatcgtatt gtttgtatca
      241 aaaatccagg atactcaaat tacacctttg ttaatgataa ttgtagtatt catagggttg
      301 gattcagtcg catatataag cggttatttc aaaaatggac aagacttgat cctcttccat
      361 attcacagag aatattgaat attgcacatg attttcctat aacgaaagaa agtgttattg
      421 tagttcataa tagcatgaag ctatacactc aaattcgtaa gcgagctcct caggcaagag
      481 tggttataca tatgcataat gcatttgaac caaaattgct tgagcaaaat gttaaaatga
      541 tagtgcctag tttatacttg gaaaagtatt accaatctta tttagcaaat gcagatattg
      601 agattgtacc aaatggaatt gatttagaaa cctatcaatc taattttcag ccgatcacta
      661 gaagcgaact taatatctcc cctgaagaga aaattatttt ttatgctggg cgtatcgtcc
      721 ctgacaaagg catattgctg ttgatgcaat cattcgaaaa attggccgct gctcacaaaa
      781 atttaaaatt agttgtgatt ggtgattata cagaaatgag taaaagtgac aaaggtgcat
      841 atcaacgcaa tgtcagggaa attgcaaaac gtttgaagga tcgttgcatt atgttaggaa
      901 gcataccgcc tgaaaagatg cattgttatt acccactggc agatttagtc gtcattcctt
      961 cccagttcca ggaaccattt tgcatggtgg caattgaagc tatgggtgct ggtaagcctg
     1021 ttttagtgag tacgcgtggg gggatgacgg aatttgtcaa agaaaatact acgggattcc
     1081 atttaaaaga acctatgact gcagactcca taagcagcga tattttaaaa acgttagcga
     1141 atcctgaatt aacagcagtt gcaaagcaag gtcaggactt tgtgtttgaa cactacagtt
     1201 gggatggcgt aactcaaaga ttcgaagagg ttattcataa ttggtttgaa taaataaaaa
     1261 ggccgcatta tgcagccttt tttatttttt acttgttttt catcgctaat aataagccgg
     1321 cgtaaacgcc taataaattt ggttcaattt gttctacgtt tccacggata acaaaataac
     1381 ctataaaag
//
LOCUS       DAZFXT010000322         1382 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.325, whole genome shotgun sequence.
ACCESSION   DAZFXT010000322 DAZFXT010000000
VERSION     DAZFXT010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.325"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..496)
                     /locus_tag="ACHKW0_005204"
     CDS             complement(<1..496)
                     /locus_tag="ACHKW0_005204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477831.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIVNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPGGKCIITGDFQKELAYLQNVIIYNDSSLRMDFFGYNALIIQRSD
                     NTCELTINEPLKNQEISTGNINVNFPLKDIYNEIRRLNLVFSCGTGGIVDLSSLDLRN
                     IDLEL"
     gene            complement(518..1057)
                     /gene="yhaB"
                     /locus_tag="ACHKW0_005205"
     CDS             complement(518..1057)
                     /gene="yhaB"
                     /locus_tag="ACHKW0_005205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YhaB"
                     /protein_id="HFO7477832.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSAVNKKELECIKETISKYCAKFTRKEAILTPPAHFNKTSIT
                     SDCWQILFFSPDHFNNDFY"
     gene            complement(1313..>1382)
                     /locus_tag="ACHKW0_005206"
     CDS             complement(1313..>1382)
                     /locus_tag="ACHKW0_005206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HFO7477833.1"
                     /translation="QLINVNFFSSDRTSFCECNRFP"
BASE COUNT          428 a          244 c          255 g          455 t
ORIGIN      
        1 aaagttctaa atcgatattc cgtaagtcca gagaggatag atcaactata cctccagtcc
       61 cacaactaaa aactaagttc aacctcctga tttcattgta aatgtctttc aatgggaaat
      121 taacattgat attgcctgtg gatatttcct ggttttttaa cggttcatta attgtaagtt
      181 cacaggtatt gtctgatcgt tgaataatta gggcgttata accaaaaaaa tccatacgaa
      241 gagacgaatc attataaata attacatttt gtaagtacgc taattctttt tgaaaatcac
      301 cagtgataat gcattttcca cctggttgta acgcgttaag taaagtgacc atttgaatag
      361 aggtctgttt tagtttttct atacctggtg ctagtgcttc gaggttatta actatggggc
      421 tttcaggaag atcattcatt tttcgacgga ctaaatcact actaatatca ttaccaattg
      481 atgaaacagg gaacattatt ttatccttta taaataatca ataaaaatca ttattaaaat
      541 gatcgggtga aaaaaagaga atttgccaac aatctgaagt aatgctggtt ttattaaagt
      601 gcgcaggagg tgttaagatg gcttcttttc gggtgaattt tgcacaatac ttagaaatcg
      661 tctccttaat acattccagc tcttttttat tgacggctga gcaagtacat tgttcaccaa
      721 aaggtgcaaa agtgcctaca tctaatgttt gtatgatata atcgtcatgt tctcctgtag
      781 aaatatctat gatgaatgga ctcatatttt ccggttggat tcggatcatg acagaaggaa
      841 catcgccttg atagaaattc aatgcagtaa ttgcagataa tactctccgc agttcattac
      901 ttatactgta aattttatta acgtcattgg tggagacaat ttctgcaaac tttcttttta
      961 ccgtccaata atcaatattt ctattgtgat tgttaaccac tgcgtgcatt ggcgggattg
     1021 aagggtgaaa aatatgtgca attgggaagc ctttcattga tgcctgcctt gtcgtcatga
     1081 caaacaataa tagatgctta ttttcctcgc agggttcttt cgcactatca aaaaacgctc
     1141 atttacccgg cgttacagta aacgaagcaa cattctgtgg cctacgacga tcaaaagctc
     1201 tgtgaaaatt tcccttgttg tctttgtctg accttatcgc ggcgatcagc aattattcgt
     1261 tttgtgatcc tcaacacaaa atgctattcg tcgcctatga ggggagaaga atttatggaa
     1321 aacgattgca ttcacaaaat gacgtcctgt cagaagagaa aaagttgacg ttaatcaatt
     1381 gt
//
LOCUS       DAZFXT010000323         1370 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.326, whole genome shotgun sequence.
ACCESSION   DAZFXT010000323 DAZFXT010000000
VERSION     DAZFXT010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.326"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..825
                     /locus_tag="ACHKW0_005207"
     CDS             <1..825
                     /locus_tag="ACHKW0_005207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HFO7477834.1"
                     /translation="VEQMQNQMDPVYTPARKIHLYHCDHRGLPLALVSTEGATEWCAE
                     YDEWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKG
                     GWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDIAKQLRLPY
                     PIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMDTKTASDYL
                     PQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRTSRGIRK"
     gene            822..>1370
                     /gene="ybfC"
                     /locus_tag="ACHKW0_005208"
     CDS             822..>1370
                     /gene="ybfC"
                     /locus_tag="ACHKW0_005208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YbfC"
                     /protein_id="HFO7477835.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNSIMVKYFKDEKPTYA
                     RMTAHLPNKNESYECLIEIQHEL"
BASE COUNT          436 a          282 c          323 g          329 t
ORIGIN      
        1 gtggagcaga tgcaaaacca gatggacccg gtgtacacgc cggcgcgaaa aatccacctg
       61 taccactgcg accatcgcgg cctgccgctg gcgcttgtca gcacggaagg ggcaacagaa
      121 tggtgcgcag aatacgatga atggggcaac ctgctgaatg aagagaaccc gcatcagctg
      181 cagcagctta tccgcctgcc ggggcagcag tatgatgagg agtccggcct gtattacaac
      241 cgccaccgct attatgaccc gctgcagggg aggtatatca ctcaggatcc gattgggctg
      301 aaggggggat ggaattttta tcagtatccg ctgaatccgg ttcagtatat agattcaatg
      361 ggactggcat caaaatatgg acacttaaat aatggcggat atggagcgag acccaacaaa
      421 ccgcctacgc ccgatccaag taaattgccg gacatagcga aacaattaag actgccatat
      481 cctattgacc aggccagtag tgcgcctaat gttttcaaaa cattcttcag agcattaagc
      541 ccttacgact acacactgta ttgcaggaag tgggtaaaac caaatctgac ttgtacgcca
      601 caggatgatt cccagtatcc agggatggat acaaagacag caagtgatta cctgccacag
      661 acaaattggc caacaactca attaccacca ggatatactt gtgcagaacc ctatttattc
      721 ccagacatta ataaacccga tgggccagca acagcaggga tagatgattt gggtgaaatt
      781 ttagctaaga tgaaacagag aacatcgaga ggaataagaa aatgaaaaga gttttgttct
      841 ttttgctgat gatatttgtt agttttggtg ttatagctga ttgcgaaata caagctaaag
      901 atcatgattg ttttactatt ttcgctaagg gtactatttt ttccgcgttt cctgtcttaa
      961 ataataaagc catgtggaga tggtatcaga atgaggacat cggtgagtat tattggcaaa
     1021 cagaactggg tacatgtaaa aataataaat tcacaccaag cggggcgaga ctcttaattc
     1081 gcgtcgggtc actacgcctt aatgaaaatc atgccatcaa aggaacgttg caggagttga
     1141 taaatacagc agaaaaaacg gcgtttctcg gcgatcggtt tagaagttat ataagagcgg
     1201 gtatctatca aaaaaagagc agtgatccag ttcagttgtt ggcagtcctt gataattcaa
     1261 tcatggtaaa atattttaaa gatgagaaac caacttatgc tcgaatgaca gcacatctcc
     1321 cgaacaaaaa tgagtcttac gaatgtttaa tagagataca gcatgaacta
//
LOCUS       DAZFXT010000324         1362 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.327, whole genome shotgun sequence.
ACCESSION   DAZFXT010000324 DAZFXT010000000
VERSION     DAZFXT010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1362)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1362)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1362
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.327"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..365
                     /locus_tag="ACHKW0_005209"
     CDS             <1..365
                     /locus_tag="ACHKW0_005209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7477836.1"
                     /translation="VDGKPVIPGMKLSKEKCDRVNAIERDKALAWVERNIKVPLTEPQ
                     KAGIASFCPYNIGPGKCFPSTFYKRLNAGDRRGACEAIRWWIKDGGRDCRIRSNNCYG
                     QVSRRDQESALACWGIDR"
     gene            640..1210
                     /locus_tag="ACHKW0_005210"
                     /pseudo
     CDS             640..1210
                     /locus_tag="ACHKW0_005210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309241.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor N-terminal domain-containing
                     protein"
     gene            1210..1356
                     /locus_tag="ACHKW0_005211"
     CDS             1210..1356
                     /locus_tag="ACHKW0_005211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477837.1"
                     /translation="MGWLMISDKLITLVKSLCVLVGISFLVMLVAIFFSTAWRVLTLS
                     GLVG"
BASE COUNT          373 a          279 c          377 g          333 t
ORIGIN      
        1 tggtggatgg taagcctgtg attcctggca tgaagctgtc gaaggaaaaa tgcgaccggg
       61 ttaacgctat cgaacgggat aaggcgctgg catgggtgga gcgtaatata aaagttccac
      121 tgaccgagcc acaaaaagca ggtatcgcgt cattttgccc ctataacatt ggccccggta
      181 agtgtttccc gtcgacgttt tataagcggc ttaatgcagg cgatcgcagg ggagcgtgtg
      241 aggcgattcg ctggtggatt aaggacggtg gcagagactg ccgtatccgt tcaaacaact
      301 gttacggtca ggtatcaaga cgtgaccagg agagtgcgct ggcatgctgg ggtatcgaca
      361 ggtaagcaga atattttgct gaaaaatgac gttggccaac gcgggtagac aacacgaaat
      421 cctgcgaact ggcaaaatgt aagtgaataa agtcaacaag attgtttcat gaagaggcac
      481 cgtaatggtg cctttgtcat ttctgcgctt cgcacaagcg taaataaacc aaagaacctt
      541 tcaggatgag ccctggtgga taaccggcag tggtctggtt aaccctcttt gggctggtta
      601 ttcctgtgcg cagggttcat cactaaaagg aaataaccga tgaatatgat gaccgtgccg
      661 tttcacggca actctcttta tgtagttaac cataatggcg aaccatacgt tcccatgaaa
      721 cctgtcgttg cggggatggg gctggcctgg caatcacagt tggctaagtt aagacagcgt
      781 tttgcgtcaa ctataacgga aatcgttatg gttgctgagg atgggaaaca acgcaatatg
      841 gtgtccatgc cacttcgaaa acttgccggc tggctacaaa ccattaatcc caacaaagta
      901 aaacccgaaa tccgcgataa ggtcatccgg tatcaggaag agtgcgacga tgttctttac
      961 gagtactgga cgaagggttt tgtcgttaat ccccgtaaaa tgagcgtgat ggaagaactc
     1021 aacccggctt ggtgctgaca tgaaacggga taaaaacatt gccagtgtgt ttgctaccgg
     1081 gctgaatgag tggaaacagg ttaaagccgc gcatgtatca aaaatccgta cgctggtaaa
     1141 tgaagcgaat atgctgattg attttgtcct ggctgataca ggcaaaggga aaataacaaa
     1201 ggcggattga tggggtggct aatgatatca gataaactca taacgctggt gaagagcctc
     1261 tgtgtacttg tcggcatttc atttttagtc atgctggttg ccattttctt ttccaccgcc
     1321 tggcgagtcc tgacgttatc gggactggtg gggtgaaaga ga
//
LOCUS       DAZFXT010000325         1360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.328, whole genome shotgun sequence.
ACCESSION   DAZFXT010000325 DAZFXT010000000
VERSION     DAZFXT010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.328"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHKW0_005212"
     CDS             complement(<1..99)
                     /gene="hokD"
                     /locus_tag="ACHKW0_005212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HFO7477838.1"
                     /translation="MKQQKAMLIALIVICITVIVTALVTRKDLCEVR"
     gene            complement(345..449)
                     /locus_tag="ACHKW0_005213"
     CDS             complement(345..449)
                     /locus_tag="ACHKW0_005213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477839.1"
                     /translation="MSDDISLAMEGALAVIAVVGVYCLVVFLMDRLGN"
     gene            complement(565..1149)
                     /locus_tag="ACHKW0_005214"
     CDS             complement(565..1149)
                     /locus_tag="ACHKW0_005214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HFO7477840.1"
                     /translation="MITFTKEQLISHVSENVKAMKFAVKQTVFKNSLEAIELDLALAL
                     VAQASLEAEPVLYMNRFTGKTFSLEEQPGADKEPEIYVPLYAAPPDSAAMLQAGNFRE
                     NKGSSTNNFREISETSTNYPVTPDGWISCSERMPDDGQHVIILCDGAFVLYAQYRDGE
                     FFDVVRNGDEFFETQSRNVTDWMPLPEPPQEVRQ"
     gene            complement(1206..>1360)
                     /locus_tag="ACHKW0_005215"
     CDS             complement(1206..>1360)
                     /locus_tag="ACHKW0_005215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7477841.1"
                     /translation="TDQTLIRKLPDGEIRRYDRQQNIICGECRKSEVMLRVLAFYQGN
                     FQEAVL"
BASE COUNT          345 a          365 c          276 g          374 t
ORIGIN      
        1 tcgtacctcg cagaggtctt tcctcgttac cagtgccgtc actatgacgg tgatacagat
       61 gacgatcagg gcgattaaca tcgccttttg ctgcttcata gcctgcttct ccttgccttt
      121 cggcgcgtaa gaggctaacc tacatatgtc tagcatgaaa ttggcctcag attaatgtta
      181 ggcgtcttgc aggacgcgta atgttaactg gggcttttct ctgtctgcct tacggtggca
      241 tgcccgaggc agacagcctc aagcacccgc agcaattcta cttaactctc gctttaccgc
      301 aaaccgtttt tacccgatat gggaattccc atatcgtaat gaattcagtt ccctagtcga
      361 tccatcaaaa acacaaccag gcagtaaacg cccacaacag caataacagc cagagcacct
      421 tccattgcca gtgaaatatc atccgacata ttccctcctt tggtgtgaat cccggcgaac
      481 gtttttaccc ccaccgacaa ataacatata ctaaaaaatc aatagctata gcaacgcctg
      541 taattgcaaa ggcttcaggc cagatcattg gcgcacctcc tgcggcggtt ctggtagcgg
      601 catccagtcg gttacattgc ggctctgtgt ttcgaaaaat tcatcaccat tacggactac
      661 atcaaaaaac tcaccgtctc gatattgcgc ataaagaacg aatgcgccat cacataaaat
      721 aattacgtgc tgaccatcat ccggcattcg ctcactacag cttatccaac catccggagt
      781 taccggatag ttggttgacg tttccgagat ttcccgaaaa ttattggttg acgaaccctt
      841 attttcccga aagtttccag cctgaagcat ggcggcgctg tctggcgggg cagcatatag
      901 cggcacgtat atttccggtt ccttatcagc accgggttgc tcttccagtg agaatgtctt
      961 tccggtaaat cgattcatat aaagcacggg ctctgcttcc agcgaagcct gagcaacaag
     1021 ggccagtgct aaatccaact caattgcctc gagagaattt ttgaatactg tctgttttac
     1081 tgcaaatttc atcgccttta cattttcact aacatgactg attaactgct cttttgtaaa
     1141 agtgatcatc tcattctcct ttgatgcgaa tgccagcgac aattgaagcc tgatagctaa
     1201 ttcactcaca gtaccgcctc ctgaaaattg ccctgataga acgccagtac acgcagcata
     1261 acttcactct tccggcactc gccacagatt atgttctgtt gtctgtcgta gcggcgtatt
     1321 tctccgtctg gtaactttcg aatcaatgtc tggtcggttg
//
LOCUS       DAZFXT010000326         1355 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.329, whole genome shotgun sequence.
ACCESSION   DAZFXT010000326 DAZFXT010000000
VERSION     DAZFXT010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.329"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..467)
                     /locus_tag="ACHKW0_005216"
     CDS             complement(<1..467)
                     /locus_tag="ACHKW0_005216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO7477842.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLTQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYD"
     gene            complement(1288..>1355)
                     /locus_tag="ACHKW0_005217"
     CDS             complement(1288..>1355)
                     /locus_tag="ACHKW0_005217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5445 family protein"
                     /protein_id="HFO7477843.1"
                     /translation="DMLGVGDAININTNKNIRQVC"
BASE COUNT          410 a          311 c          272 g          362 t
ORIGIN      
        1 aaatcgtaat cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg
       61 aacagcggac tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg
      121 atgtcctcgt tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg
      181 tgcaccttca tgctgtacag catctggttt ttgtcgttct cccccagttc aaaccaggtc
      241 accacacctt tcacccggcg ctgtacgtca tcgccctgcc ataccgtcag gtaggccatt
      301 ttgtccagca cctgcgcaaa ttcaagggaa agaaactgct gactgaccag agaaaggtcg
      361 agggaaaaaa gtgaagagag tgactgggtg agatgaaagg aaaccaccgc aaacgcgtcc
      421 ggtggcaggc cgtccacttc aagtgtgaaa cgtaatccgg ttgacatagc aacctccgtg
      481 aaaatgaata ataatccgca catattgcat gtacaacatg tcgggcggat gagataagcg
      541 agtcgacatc tggcgttacc ggggaatatc agaaaaatgt catggttatg tgatgattta
      601 gtctgggatt ttacttcaga gaaacgggtg tctgtttagt caacaaaata atcacaaacg
      661 caacggatgg gttctcgacg ttattcacga caggctcgta cattacagcg agtaataaaa
      721 aacctgatat gcaaagtcaa accaatattc ataatgtctt tttattcaca acaataaata
      781 attcaataac aatcattgaa atgaatgatg atttgtataa ggaatatacg ctaccgcccc
      841 accctgataa catgatctgc acattgccat cttaacgtcg ttaaacactg gcttaatagt
      901 ggatatttca aattaggaat ttattaaatt cacctgatga atatcataag caaatagcat
      961 agaaaataat gacaattccc ccgaggggac catttccaaa gcactgcacc accacctggt
     1021 gcccggatga tcttgcttca gtggtaagag tacctgattc attgttccca aaatgatgtt
     1081 ttccccggcc ccgccaataa tgtgcagctc tcacgtgata atctattgat taggttatat
     1141 taagaatcaa catttaaaat acatccattt tcttgaataa atcaacatgt ttattaatca
     1201 attgctgttt tagctctgac tcatcaagct ctccatattt gtcctacaga taattgcgaa
     1261 taaatacaga gaacgccatt tatcctttta gcatacctgc cggatatttt tatttgtatt
     1321 aatattaatt gcatctccaa ctccgagcat atcca
//
LOCUS       DAZFXT010000327         1354 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.330, whole genome shotgun sequence.
ACCESSION   DAZFXT010000327 DAZFXT010000000
VERSION     DAZFXT010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.330"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..408
                     /gene="ymfL"
                     /locus_tag="ACHKW0_005218"
     CDS             <1..408
                     /gene="ymfL"
                     /locus_tag="ACHKW0_005218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmfL"
                     /protein_id="HFO7477844.1"
                     /translation="RADGDQIFPLGWAMILQRAGGTHFIADAVAQSANGVFVSLPDVE
                     DVDNADINQRLLEVIEQIGSYSRQIRSAIEDGVVEPHEKTAINDELYLSISKLQEHAA
                     LVYKIFCISESNDARECAAPGAVACRDCGETNA"
     gene            584..763
                     /locus_tag="ACHKW0_005219"
     CDS             584..763
                     /locus_tag="ACHKW0_005219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001761574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HFO7477845.1"
                     /translation="MRQVNRWFKDHYGVPVRVIRWEPETQRVIYLREGYEHECFSPLE
                     QFRRKFREIEVGHEH"
     gene            753..>1354
                     /locus_tag="ACHKW0_005220"
     CDS             753..>1354
                     /locus_tag="ACHKW0_005220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HFO7477846.1"
                     /translation="MSTKLTGYVWDGCAASGMKLSSVAIMARLADFSNDEGVCWPSIE
                     TIARQIGAGMSTVRTAIARLEAEGWLTRKARRQGNRNASNVYQLNVAKLQAAAFSQLS
                     DSDPSKSDASKSDPSKFDASKSGKKAGFHPSESGGDPSVKSKHDPSDKKPSRPDASQP
                     DTQTDEQDFLTRHPDAVVFSPKKRQWGTQDDLTCAQWLWK"
BASE COUNT          347 a          316 c          375 g          316 t
ORIGIN      
        1 cgtgccgatg gcgatcagat tttcccgctg ggatgggcaa tgattttgca acgtgctggt
       61 ggaactcact tcattgctga cgctgtggcg cagtctgcaa atggcgtctt tgtgtctctt
      121 cctgacgtcg aggatgtgga caacgccgat attaaccagc gcctgctgga agtcattgaa
      181 cagattggca gttattccag acagattcgt tcggcaatcg aagacggtgt ggtggaaccg
      241 catgagaaga cagcaattaa cgacgagctg tatctctcaa tttcgaagct gcaggagcat
      301 gcagcactgg tctacaaaat cttttgcatt tcagaaagta atgacgcccg cgagtgtgca
      361 gctccgggcg ccgtggcgtg tcgtgactgt ggagaaacta acgcatgaac agtttaacaa
      421 cacactaccg tcgctcgcaa ctgattgcgc ttcctgtacc gggtggaaaa gcgaaggtgg
      481 agtattgcta tgcagttaat gtaccaggtg acagggaaat tgtaacccac agctttgctg
      541 agtgggctgt gggtgatttc aaccggcaga aggagacagt cctttgcgac aagttaaccg
      601 ctggttcaaa gatcactacg gagtgcccgt cagagtcatt cgttgggagc cggaaacaca
      661 acgagttatc tacctccgcg aaggctatga gcatgagtgc ttcagcccgc tcgaacagtt
      721 tcgtcgtaaa ttcagggaaa tagaggtcgg tcatgagcac taaattaacc ggctatgtat
      781 gggatggttg cgctgcatca ggcatgaaat tatccagcgt ggcaattatg gcccgcctgg
      841 ctgatttcag taatgacgaa ggtgtgtgct ggccatcaat tgaaactatt gcccgtcaga
      901 ttggcgcggg gatgagtacc gtcaggacgg ctatcgcacg gctggaagca gaaggctggt
      961 taacgcgtaa ggcgcgtcgc cagggtaacc gcaatgcgtc gaatgtttat cagcttaacg
     1021 ttgcgaagct tcaggcagcg gcattttctc aactgtcaga ttctgacccg tcaaaatctg
     1081 acgcatcaaa atctgacccg tcaaaatttg atgcgtcgaa atctggcaaa aaagcgggtt
     1141 ttcacccgtc agaatctggc ggggatccgt cagtaaaatc aaaacatgat ccgtcagata
     1201 aaaaaccttc tcgtccggac gcttcgcaac cggacacgca gacggatgaa caggattttt
     1261 taactcgcca tcctgatgcg gttgtattca gccctaaaaa gcgccagtgg ggaacgcagg
     1321 atgatttgac ctgcgcacag tggctctgga aaaa
//
LOCUS       DAZFXT010000328         1312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.331, whole genome shotgun sequence.
ACCESSION   DAZFXT010000328 DAZFXT010000000
VERSION     DAZFXT010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.331"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..112
                     /locus_tag="ACHKW0_005221"
     CDS             <1..112
                     /locus_tag="ACHKW0_005221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HFO7477847.1"
                     /translation="AIPFEGERHNALDDARYQAKYVSVIWQKLIPSQADF"
     gene            171..374
                     /locus_tag="ACHKW0_005222"
     CDS             171..374
                     /locus_tag="ACHKW0_005222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
                     /protein_id="HFO7477848.1"
                     /translation="MSSLFLTEDELLILTGCKYASHQRKWLTENGLPFYTNRSGKPIV
                     SRDLFTCNKTLPPREVEPNFGAI"
     gene            374..>1312
                     /locus_tag="ACHKW0_005223"
     CDS             374..>1312
                     /locus_tag="ACHKW0_005223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7477849.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKRYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKGNPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQ"
BASE COUNT          442 a          271 c          301 g          298 t
ORIGIN      
        1 ggctattcca ttcgaaggtg agcgccataa tgcacttgat gacgcccgtt accaggcaaa
       61 atacgtttca gttatctggc aaaaactgat cccgagtcag gctgattttt aatgttcaac
      121 cctaattgcc gctaaccgta tatagttagc ggcggttatg agatatagct atgagtagct
      181 tatttttaac cgaagatgaa ttgctaatat taacgggctg caaatatgca agccaccagc
      241 gaaaatggtt aacggaaaac gggcttccgt tctataccaa tcgtagtggc aaaccgattg
      301 tcagccggga tctatttacc tgcaataaaa ctttaccacc acgcgaggta gagccgaatt
      361 ttggtgcaat ctgatgggaa gacgaaggaa aaatcctgaa cacgaaaaac tacctccaaa
      421 tgtataccca aataaatata gttatgtatg gaaaccaaca tccagagaat ctgtcacact
      481 aaccgccatc aaggatggtt tagctgcttt atggaaaagg tatgaggaaa ctgtaaataa
      541 tcgcgatcgc gcaatgacat tcggtcgctt gtgggaaaaa ttcctcgcca gcgcctatta
      601 cagtgacctc agtccaagaa cacaaaaaga ttatctacaa catcaaaaaa agttgcttgc
      661 cgtattcggt aaggtgccgg cagattccat aaaaccagaa cacatccgtc gatacatgga
      721 caagagaggg gagcagagta aaacgcaagc caaccatgaa aaaagcagta tgtcccgcgt
      781 ttacagttgg gggtatgagc gagggtacgt gaaaggtaac ccatgtgcag gtgtaagtaa
      841 attcaaggcc aaaaaccgcg aacgatatgt aaccgacaaa gaataccagg cagtattaag
      901 cgttgcacct cttcctgttt ttatcgcaat ggaaattgcc tatctgtgtg cagcgagggt
      961 ttccgatgtg ttatcgctga aatgggagca gattggaaac gacggaatct ttatccagca
     1021 agggaaaaca ggaaaaaaac agataaaagc atggagtcca cgattacagg cggcgatcga
     1081 aaaagcaaaa cagttaccaa catccgccta tgtaatcagt aatcaatacg gcaaccgata
     1141 tatgtacaaa ggctttaacg aaatgtgggt agaagcaaga aatcgcgcag gcaaaatttc
     1201 aggtatttta accgacttca cctttcatga tctgaaggcg aaaggaattt cagactatga
     1261 aggaagcagt cgggataagc aacttttctc tggtcacaaa accgaggggc aa
//
LOCUS       DAZFXT010000329         1303 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.332, whole genome shotgun sequence.
ACCESSION   DAZFXT010000329 DAZFXT010000000
VERSION     DAZFXT010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.332"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1303)
                     /locus_tag="ACHKW0_005224"
                     /pseudo
     CDS             complement(1..1303)
                     /locus_tag="ACHKW0_005224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          264 a          417 c          301 g          321 t
ORIGIN      
        1 tcgggggtga tacttttacc gtccgccggt accggtattt tcccgaccgc atcattcacc
       61 gcctgctgca gtacatccgg atcatagtca cgaccatcac gcggaacagg gatatggctt
      121 gctgcgtcct tcaccatctg ctcaagcatc ggacgcacat catcacccgt cacgcacttc
      181 tgtaatacca cagactgaga agccagtttt tcttcaaacg tttgtgcctg cgcggctatt
      241 ttctcctcaa atgtacgctg taaatccgcc agcaccatgg cgaattcttc gcccagtgca
      301 cgaataatgg acagttcccg ttccgtcatt ttcgcagtat ccccctgaac atcgctttca
      361 ccgcatcatg ctctgtttca ctgattgcct tattaccgtc agatgcgcca tcaggcagtt
      421 gtgctgagac tgttttcccg gacgacgcga acggatcctc acgggcatca cgacgggaca
      481 gcgcctccag actgtagttc tgctgctgaa gatacagtgc atcacctccc gcaaggggcg
      541 gcaggttctc acgtttacgg gcctcattgg gcgtcagcag cgtatttttc accgactcac
      601 ccagcgtttt catgcgccgt tcgctgtcca ttctcagcag cgtggtgacg tcaaactccg
      661 tactctcgtt ttcccccgtt tccagcgcct catccagtaa cagctcaatg gactcaatca
      721 gcgtctgcag acactgggaa taatactgct gctccagcgc ctccacgtta tcactggagg
      781 gaggttgtcc cacaccaatc ttgtaggccg ggacacggaa caccgaacag acaatttcag
      841 cggtcatctt cagttgttcc accgtctgcg catccaccgg tgaaaacgtc gtggggttgt
      901 attttgcccc gttgctcaga atggccgttt tccccgcatt ttcgccggta tacccgctgt
      961 cccagttgct cttcagtttt ttcgcatttt cttccgttat actgccgggg atctcaatca
     1021 ccccggacgg cctgccgcca tttctgaaaa aagacgttga atttgcctga atatgatgcc
     1081 cctgcgtggc cgccagcccg gcagcataca ccggcggcag ccccacaagc ggatgaaaaa
     1141 aacagttaaa ccggtcgtgg atcacttccc tggcaggcac cgtcaccgcc tccgtgatcc
     1201 cgcagttccg gtccggtgta atgcgataga acacctcgcc gtcatccgcc accagaggtt
     1261 caacccggct ccagtccaga atacgcagtt ctttgatctg ccc
//
LOCUS       DAZFXT010000330         1299 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.333, whole genome shotgun sequence.
ACCESSION   DAZFXT010000330 DAZFXT010000000
VERSION     DAZFXT010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1299
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.333"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..463)
                     /locus_tag="ACHKW0_005225"
     CDS             complement(<1..463)
                     /locus_tag="ACHKW0_005225"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001429500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0046797 - viral procapsid maturation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HFO7477850.1"
                     /translation="MTLKRACSLLTVKSFSEDERVITGIASTPSPDRDGDILEPEGAE
                     FGSTIPFLWQHDHSRPVGQCTVRRVREGLEITAMLVKPEPGMPSQMAARLDEAWAAIK
                     TGLVRGLSVGFRPHEYTYLDGGGLHFLRWELMEVSAVTVPANAECTIRTIKY"
     gene            complement(527..>1299)
                     /locus_tag="ACHKW0_005226"
     CDS             complement(527..>1299)
                     /locus_tag="ACHKW0_005226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="terminase TerL endonuclease subunit"
                     /protein_id="HFO7477851.1"
                     /translation="REAGEEAFRGFMSKHANVEIGLALRSDRWAGADFWEQQGRRVSL
                     DDILQRADVVTVGIDGGGLDDLLGMYVTGRDRETREWLGWGHAWVHETAVVRRKSEAS
                     RFQDFVACGDMTIVRRVGDDTAEVAEYVRRIHEAELLDHIGIDPSGVGQILDSLAEAG
                     IPDESVVGISQGWKLGGAIKTTERKLAEGVLIHGDQPLMAWCVGNARVEPKGNAILIT
                     KQASGRGKIDPLMALFNAVSLMSLNPEPKKKAYEVFFI"
BASE COUNT          282 a          450 c          313 g          254 t
ORIGIN      
        1 aatatttaat ggtccggatg gtgcattccg cattcgcggg cacggtgacg gcagacacct
       61 ccatcagctc ccagcgcaga aaatgcagtc cgcctccgtc cagataagtg tattcatgag
      121 gccggaagcc cacagaaagc cccctgacca gcccggtctt aatggcagcc caggcctcat
      181 ccagccgggc tgccatctgg gagggcatcc ccggctccgg cttcaccagc attgccgtga
      241 tttccagccc ttccctgacc cgacgcaccg tacactggcc tacagggcgg gaatggtcat
      301 gctgccagag aaacgggatc gtactgccaa actccgcccc ctccggctcc aggatgtcac
      361 catcccgatc cggagaaggc gttgacgcaa tcccggtgat cacccgttca tcctcactga
      421 aggatttcac cgtcagcagg gaacaggccc gtttaagagt cacatcagcc tcctgaaaat
      481 aaaaaaaccg ccgcagcggt tcatgatggt tacagggtga gcagggttat atgaaaaaaa
      541 cctcatacgc tttctttttc ggttccggat tcagggacat cagggacacc gcattgaaga
      601 gcgccatcag cgggtcaatt tttccccgtc cgctggcctg tttggtgata agaatggcgt
      661 tacctttagg ctccacccgg gcattaccga cacaccaggc catcaggggc tgatcaccgt
      721 gaatcagcac cccttcagcc agtttgcgct cggtggtttt gatggccccg cccagcttcc
      781 agccctggct tatccccacc acactctcat cggggatccc ggcttccgcc agtgaatcca
      841 gaatctgccc cacacctgac gggtcaatac cgatatgatc cagtaactca gcctcatgaa
      901 tacgacgcac atactccgcc acttccgccg tgtcatcccc gacccgacgg acaatcgtca
      961 tgtctccaca ggccacaaaa tcctgaaacc gggatgcctc actcttccgt ctgaccaccg
     1021 cggtttcatg cacccaggca tggccccagc ccagccattc gcgggtttcc ctgtcacggc
     1081 cagtcacgta cattcccagc agatcatcca ggcccccgcc gtcaatcccc accgtcacca
     1141 catcagcgcg ctgcaggata tcgtccaggc tgacgcgcct gccctgctgc tcccagaaat
     1201 ccgcgcccgc ccagcggtca gaacgcaggg caagaccgat ttccacattg gcgtgttttg
     1261 acatgaagcc acgaaatgct tcctcaccag cctcccggg
//
LOCUS       DAZFXT010000331         1298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.334, whole genome shotgun sequence.
ACCESSION   DAZFXT010000331 DAZFXT010000000
VERSION     DAZFXT010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.334"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            553..>1298
                     /locus_tag="ACHKW0_005227"
     CDS             553..>1298
                     /locus_tag="ACHKW0_005227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HFO7477852.1"
                     /translation="MTKHMDIHCQTRIFNSINHDTKYKICIFTPKNIKKNNATLYILD
                     GNSANNYISDILPVIDALPNPPVLVTLGYESWNNLSIHRRAYDYTPDGENAIVDNSKP
                     AWIYFTGGGSQSFRELLLTQIMPWVSTIAPNSSRIGIWGHSLGAIFVLDCLKNNSCFN
                     YYYISAPSLLWQNERIIKIIKDDISESKHTKSICLLNGNLSLDYSASLYPEAIKAESV
                     LRNILTEKYSNFSIVQFPELNHQETFSAAL"
BASE COUNT          420 a          251 c          229 g          398 t
ORIGIN      
        1 ttcatagatt gagcctcaga ttaatgttaa gcgtcttgca ggacgcgtaa tgttaactgg
       61 ggcttttctc tatctgccgt tggtgttcat gcccgaggca gatagcctca agcacccgca
      121 gctattctac ttaacttccc attacctcgc caatatgaaa tcggtcagaa agacagacgt
      181 cccataagga caacaacgga aaaagagatt ttgcttgatg ctatttacct ctgcaccatg
      241 aagttgcgcc aaaatcccag tggtgataac aggaatttgg ttgttggtga ttggggaaag
      301 aagtacgtta gactgtgggt tagttcattg acatgatagt tacctgcctt caatttccta
      361 agcaagcgat gtaaacgata atcaatatca aatgtattga caggtaaaat aataacgatg
      421 tacctttgct gataacatat atcagtctta aatcataatg gaggtagata aaataactaa
      481 acacacacaa caaatttttt atcagtgaca tagcatatct taagcatcct ttaatgctca
      541 tggggagaac gcatgactaa acatatggac attcattgtc aaacacgcat atttaactca
      601 attaaccatg acaccaaata taaaatatgt atttttactc caaaaaacat taaaaaaaat
      661 aatgcaactc tttatatact tgatgggaat agtgccaaca attacatttc tgatattctg
      721 cctgttattg atgcactacc caatccacca gtgttagtta cacttggtta tgaatcttgg
      781 aataacctta gcattcatcg ccgtgcttac gattatactc ctgatggtga aaatgctatt
      841 gttgataact ctaaaccagc atggatctac tttactggtg gaggaagcca gtctttccga
      901 gaattattac taactcagat catgccatgg gttagtacta ttgctccgaa cagttccaga
      961 atcggtatat gggggcactc gctaggtgct atttttgtgc ttgattgctt aaaaaataat
     1021 tcatgcttta actattatta tatatctgca ccatcacttc tatggcagaa tgaaagaatc
     1081 atcaagataa ttaaagatga tatatcagaa tcaaagcaca ctaaaagtat atgcttgctt
     1141 aacggaaatc ttagccttga ttactccgca tctttatatc ctgaagccat caaagcggaa
     1201 tctgttttga gaaatatttt gacagaaaaa tacagtaatt tttctattgt acaatttcca
     1261 gagctcaatc atcaggagac tttctctgct gcactctg
//
LOCUS       DAZFXT010000332         1285 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.335, whole genome shotgun sequence.
ACCESSION   DAZFXT010000332 DAZFXT010000000
VERSION     DAZFXT010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.335"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1023
                     /locus_tag="ACHKW0_005228"
     CDS             <1..1023
                     /locus_tag="ACHKW0_005228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001704620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7477853.1"
                     /translation="NHLEKNQGIINSKLTKFNNTIEEKIHSLLKKWADEIKVDRSDYY
                     AKTISVVDSIDNDSQIENVFNLLDSLYVESVDTLTFKYQSIIKGLDRLFEGINLDSAF
                     SLSEEERSYFEEKAKSLNALAQLGISVEIISHELEEMDSMVTRGLNSLPTSVKEHPGF
                     SLALNAHRSLTQQIRFLSPLKISGYQSRQRITGKNIMDYVLKFFGERFERQRITIEFS
                     EEFKQIAITDIPSRIYPVFTNIINNAMYWVSLSNNRLIKIGFVNSLVIIANSGPAIDT
                     DDIPRLFELFYSKRANGHGVGLYLCRENLAVAHHKIWYLEPDEGDNYLIKDGANFVIQ
                     FNGVEF"
     gene            1025..>1285
                     /locus_tag="ACHKW0_005229"
     CDS             1025..>1285
                     /locus_tag="ACHKW0_005229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator receiver domain"
                     /protein_id="HFO7477854.1"
                     /translation="MTVANYNSLVQKTFCENAIRSVVMIDDDFLTYSESIRALNNEVD
                     LDYNKIDSSKRAATLESFFQSKNMICDVDNGSVNFDVDRIRKS"
BASE COUNT          439 a          195 c          237 g          414 t
ORIGIN      
        1 aatcaccttg aaaaaaatca aggtattatt aattctaaac taactaagtt taataacacg
       61 atagaggaga agatacattc tcttttaaaa aaatgggccg atgaaataaa ggttgatcga
      121 agtgattatt atgctaaaac tatatcagtt gttgattcaa tagataatga ttcacaaatt
      181 gaaaatgtgt ttaatttgct cgatagttta tatgttgagt cagttgatac cctaactttc
      241 aaatatcaat caataataaa aggtctcgat agattatttg aaggtataaa cttagattca
      301 gcattctcat tatctgaaga agaacgctca tattttgaag aaaaagctaa aagtttaaac
      361 gcgcttgcac agttaggtat tagtgttgag ataatatctc atgaacttga agaaatggat
      421 tctatggtaa ccagaggact aaactctctt cctacttctg taaaagaaca ccctggtttt
      481 tcattggcgt taaatgctca cagatcgctt actcaacaaa tacgtttctt atcacctttg
      541 aaaatatcag gttatcaatc caggcagaga ataactggaa aaaatatcat ggattatgtc
      601 ctgaagttct ttggggagcg tttcgaacgg caacgaataa ctattgaatt tagtgaagag
      661 tttaagcaaa tcgcaataac agatatacca tcaaggatct atcctgtttt tactaatatt
      721 atcaacaatg caatgtattg ggtcagtctg tcaaataata ggctcataaa gattggtttt
      781 gtgaattctt tggttatcat agcaaattct ggtccggcaa ttgataccga tgatatcccg
      841 cgactatttg aactatttta tagcaaaaga gcaaatggac atggggtagg tctgtatcta
      901 tgtcgagaaa accttgctgt tgcacatcat aaaatatggt atttagaacc tgatgaaggc
      961 gataactatt taataaaaga tggcgctaat tttgtgatcc agttcaatgg agtggagttc
     1021 taatatgaca gtggcaaatt ataattctct tgtccagaaa actttctgcg aaaatgcaat
     1081 tcgttccgtt gtcatgattg atgacgattt tctgacgtat tctgaatcaa tcagggcgtt
     1141 gaataacgaa gttgatttag actacaacaa aattgactca tctaaacgag ccgctactct
     1201 tgagagcttt tttcaatcta aaaatatgat ttgtgatgtt gacaatggtt ctgttaattt
     1261 cgatgtggat cggattagaa aatca
//
LOCUS       DAZFXT010000333         1272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.336, whole genome shotgun sequence.
ACCESSION   DAZFXT010000333 DAZFXT010000000
VERSION     DAZFXT010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.336"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            64..>1272
                     /locus_tag="ACHKW0_005230"
     CDS             64..>1272
                     /locus_tag="ACHKW0_005230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477855.1"
                     /translation="MYNFITIMYDVFSCFGVLAKNQNSRDIRNIKNFSSHQHSLGDMF
                     DELINIIDKEQVLSKEQRKVIFRRYEDLYVKLMHYSVFTDKTHQIIKQKYFNDIVPMI
                     LALDIRNTYRPDNEMAFYYHIHSFLTQIPDNEDDIYHAARTYLRNYVKLCLSGYTPAN
                     AHFKDIFDGVYEFIRNIRKNSTPGKTKLIATINTCKETCKHLLYLSNEDKEKIISDLD
                     KVQVACYYLTILLAFERRTSLTSILATLYKMLISEREVSEYECQLLYLTNPIDVMNIL
                     NKYIYYFPNENSPFYTLKIDSALSWDAIDAIRDYSISDIYLYPEQKTINCVVEIENIV
                     FGGYIYTLNNGVTLQNIENSLKDSSCHYVLNGYTEFVNCLRQLTSGKTESVHRTINKL
                     NYEKLPFGFII"
BASE COUNT          474 a          206 c          194 g          398 t
ORIGIN      
        1 ttaacagaaa agcaaaatga tataatgtta aaattttata tagtgcaata aaaggagaat
       61 gttatgtata attttatcac tataatgtat gatgtctttt catgttttgg tgttctggct
      121 aaaaaccaga atagccgtga catccgaaat attaaaaatt tttcctcaca tcaacattca
      181 ctgggcgaca tgtttgatga attaataaac attattgata aagaacaagt attgagtaaa
      241 gaacaacgaa aagttatatt taggagatat gaagatctct atgttaagct aatgcactat
      301 tctgttttta cagacaaaac acatcaaata ataaaacaaa aatattttaa tgacattgta
      361 ccaatgattc tcgcactcga catcaggaac acatatcgcc cggataatga gatggcattt
      421 tactatcata ttcattcttt tctcactcag ataccggata atgaggatga tatatatcat
      481 gctgcaagga catatctgcg aaattacgtt aagttatgtt tatccggata cacgccagcg
      541 aatgcgcatt tcaaagatat ctttgatggc gtatatgaat tcattcgtaa tattcgcaaa
      601 aacagtacac caggaaaaac aaaacttatc gcaactatca acacatgcaa agaaacctgt
      661 aaacatctgc tttatttaag taatgaagac aaggaaaaaa taatttctga cttagataaa
      721 gttcaggttg catgttatta tctcactata ttactggctt tcgaaagacg aacttcatta
      781 acaagcatcc tggcaacttt atataaaatg ctgataagcg aaagagaagt ttcagaatat
      841 gaatgccagt tattatattt aaccaaccca atagatgtaa tgaatatact gaacaaatac
      901 atatattact ttcctaatga gaactcacca ttttatacac tgaaaattga cagtgcatta
      961 tcgtgggatg ccattgacgc aatacgagac tatagtattt ctgatattta tctttatcct
     1021 gaacaaaaaa caataaattg tgtcgttgag attgaaaaca ttgtctttgg cggttacatt
     1081 tatacattga acaacggcgt cacattacaa aacatagaaa actctttaaa agattcttca
     1141 tgccattatg tcttaaatgg ctatacagaa tttgttaact gtttgagaca acttacttca
     1201 ggaaagactg aaagtgttca tcgcaccatc aataaactga actatgagaa attacctttt
     1261 ggatttatca tt
//
LOCUS       DAZFXT010000334         1269 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.337, whole genome shotgun sequence.
ACCESSION   DAZFXT010000334 DAZFXT010000000
VERSION     DAZFXT010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1269
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.337"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1269
                     /gene="rhsB"
                     /locus_tag="ACHKW0_005231"
     CDS             <1..>1269
                     /gene="rhsB"
                     /locus_tag="ACHKW0_005231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HFO7477856.1"
                     /translation="FTDCSGYQTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVK
                     DTQGHETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRV
                     IRLTSENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDE
                     ADRLTHRTVKGETAERWRYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHH
                     PQTEALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTR
                     DRLHRETLRRFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTR
                     SYSYSTTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDA
                     HYLYRYDRYGRLTEKTDLIPEGGIRTDDERT"
BASE COUNT          297 a          407 c          377 g          188 t
ORIGIN      
        1 cttcactgac tgttccggct accagacccg ttatgaccat gaccgcttcg ggcagatgac
       61 ggcggtgcac cgcgaggaag ggctgagcca gtaccgcgca tacgacagcc gtggacagtt
      121 aattgccgtg aaagacacgc agggccatga aacgcggtat gaatacaaca tcgccggtga
      181 cctgaccgcc gtcattgccc cggacggcag cagaaacggg acacagtacg atgcgtgggg
      241 aaaggccgtc cgtaccacgc agggcgggct gacgcgcagt atggaatacg atgctgccgg
      301 acgggtcatc cgcctgacca gtgaaaacgg cagccacacc accttccgtt acgatgtact
      361 tgaccggctg atacaggaaa ccggctttga cggccgcaca cagcgttatc accacgacct
      421 gaccggcaaa cttatccgca gcgaggatga gggtctggtc acccactggc actatgacga
      481 agcagaccgc ctcacgcacc gcaccgtgaa gggtgaaacc gcagagcggt ggcggtatga
      541 cgaacgtggc tggctgacag acatcagcca tatcagcgaa gggcaccggg tgacggtgca
      601 ttacgggtat gatgagaaag gccggctgac cggtgagcgt cagacggtgc atcacccgca
      661 gacggaagca ctgctctggc agcatgagac cagacatgcg tacaacgcgc aggggctggc
      721 gaaccgctgt ataccggaca gcctgcccgc cgtggaatgg ctgacctacg gcagcggtta
      781 cctggcaggc atgaaactcg gcgacacacc gctggtggag tacacccgcg accgcctgca
      841 ccgggaaacg ctgcgccgct tcggccgtta tgaactcacc accgcttata cccctgccgg
      901 gcagttacag agccagcacc tgaacagcct gctgtctgac cgcgattaca cctggaacga
      961 caacggcgaa ctcatccgca tcagcagccc gcgccagacc cggagttaca gctacagcac
     1021 caccggcagg ctgaccggcg ttcacaccac cgcagcgaac cttgatatcc gcatcccgta
     1081 tgccacagac ccggcaggta accgcctgcc cgacccggag ctgcacccgg acagcaccct
     1141 cagcatgtgg ccggataacc gtatcgcccg tgacgcgcac tatctttacc ggtatgaccg
     1201 ttacggcagg ctgacggaga aaaccgacct catcccggaa gggggtatcc gtacggatga
     1261 tgagcgcac
//
LOCUS       DAZFXT010000335         1263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.338, whole genome shotgun sequence.
ACCESSION   DAZFXT010000335 DAZFXT010000000
VERSION     DAZFXT010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.338"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            140..382
                     /gene="yjbE"
                     /locus_tag="ACHKW0_005232"
     CDS             140..382
                     /gene="yjbE"
                     /locus_tag="ACHKW0_005232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide production protein YjbE"
                     /protein_id="HFO7477857.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            496..1134
                     /gene="yjbF"
                     /locus_tag="ACHKW0_005233"
     CDS             496..1134
                     /gene="yjbF"
                     /locus_tag="ACHKW0_005233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HFO7477858.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1131..>1263
                     /locus_tag="ACHKW0_005234"
     CDS             1131..>1263
                     /locus_tag="ACHKW0_005234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477859.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     "
BASE COUNT          307 a          329 c          337 g          290 t
ORIGIN      
        1 tttgatattc attccaatta tcctaaaacc ccatctctaa ttccccgcgc cgtaattcgc
       61 atgctttagt tgtgtatact cgatcccgcc cgaaatgttt ttgggtaaat ctccattcat
      121 tcaatgaagg gaaattgtta tgaaaaaagt tctgtatggc atttttgcca tatctgcgct
      181 tgcggcgact tctgcgtggg ctgcacctgt acaggtgggc gaagcggcag ggtcggcagc
      241 aacgtcggtt tcggcgggga gttcctccgc gaccagcgtc agcaccgtaa gctcggcggt
      301 gggtgtcgcg ctcgcggcaa ccggtggcgg tgatggttct aataccggga ccaccacaac
      361 cacgaccacc agtacccagt aataaagtat gtatccccaa aataatttga gtcattgcat
      421 ctgtggcttg aagtatgaag ggatttacca taaccacact ccggtgtggt tattctgccc
      481 ctctggagaa gagtcgtgaa gcgacctgca ctcattctta tctgcctgct attacaggcc
      541 tgttcagcca cgactaaaga gctgggcaat tcactgtggg acagtctgtt cggcacgcca
      601 ggcgtacagc tgacggacga tgatattcaa aatatgccct acgccagcca gtacatgcag
      661 cttaatggcg ggccgcaatt gtttgtggtg ctggccttcg ctgaagacgg acaacaaaaa
      721 tgggtcactc aggatcaagc cactcttgtc acacaacatg gtcgtctggt gaagactttg
      781 cttggcggcg acaacctgat tgaagtgaat aaccttgccg ccgacccgct gattaaaccc
      841 gcacaaattg ttgatggtgc aacatggacg cgcacgatgg gctggaccga gtaccagcag
      901 gtacgctacg ccaccgcacg ctcagtcttc aaatgggatg gcaccgatac cgtcaaggtc
      961 ggcagcgatg aaaccccggt tcgcgtgctg gacgaagaag tctccaccga ccaggcgcgc
     1021 tggcataacc gctactggat cgacagcgaa gggcaaattc gccagtcgga acagtatctc
     1081 ggcgcggatt atttcccggt gaaaaccact ctcatcaagg cggcaaaaca atgattaaac
     1141 aaactattgt cgcattgatt ttgagtgtgg gggcgtcatc ggtcttcgcg gcgggaaccg
     1201 tcaaggtatt cagcaatggc agcagtgagg ccaaaacgct gacgggcgca gagtatttaa
     1261 tcg
//
LOCUS       DAZFXT010000336         1255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.339, whole genome shotgun sequence.
ACCESSION   DAZFXT010000336 DAZFXT010000000
VERSION     DAZFXT010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.339"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            590..880
                     /locus_tag="ACHKW0_005235"
     CDS             590..880
                     /locus_tag="ACHKW0_005235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7477860.1"
                     /translation="MNLSASMNDAGDGFELLMSGNMQDFNICCRRDNHYPEHKLIPLS
                     ELFDVNYGLNLELNKLEKDSSGINFVSRTSKNNGVSARVKLKDGISPCQQLC"
     gene            904..1155
                     /locus_tag="ACHKW0_005236"
     CDS             904..1155
                     /locus_tag="ACHKW0_005236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HFO7477861.1"
                     /translation="MESFVQDSPFYSGRDLYWLRPKVELTLEEKLYYCSCIRRNRHKY
                     SYGRQANRTLKNLLVPSLDSVPAWVYGVTGKIISELSER"
BASE COUNT          349 a          227 c          296 g          383 t
ORIGIN      
        1 aattaaaaaa cataacgaaa ttttgcctca ttctgcattt cattgaaata gtatgtttct
       61 catcgaaagc aagtttgtca atataaattt gtaacagaaa tgactttccg gggataaaca
      121 aaactgttca tggcaatggc tttctctgtc gccaacatgg atacattgtt ccggataaag
      181 cactgatgtc attgactgac gcagactatc aggccatctg atgctttctt ttccttcacc
      241 ggtattgatg atatgcctta tgaaggggga cttattcctg taacccaatc acaggcactg
      301 tgattactgg tttatgattg atgctgattt gagcagatat gcacagcagt tgtggattca
      361 ggggatattt tctgccagat attcatacca gattgatgac ctctttgctc agatgctctt
      421 cgttctgagc cagccaggat tttgtggcca catacattgt tcgccgatgg ggaatcgaga
      481 caggcgattg gctgtgatgc gattttgccg ctaactgagg atgagtccgc ccctcccggg
      541 gaattcctgt aagttaagcc gtacctgttg attagcacta aactgggtaa tgaatttatc
      601 tgccagtatg aatgatgccg gggacggttt tgaactcctg atgtcgggta atatgcagga
      661 ttttaatatt tgttgtcgtc gggataatca ttatcctgaa cataaactga tacctttatc
      721 agagttattt gacgttaatt atggactgaa tcttgagctg aataaactgg agaaagattc
      781 ttcaggaatt aattttgttt caagaacgtc caaaaataat ggcgtctccg ccagggttaa
      841 attaaaagat ggtattagtc catgccagca gctgtgttaa ctgtagcagc aggaggttct
      901 gttatggaat cgtttgtaca ggattcacca ttttatagtg gaagagatct ttattggtta
      961 cgtcctaaag ttgaactgac tctggaagag aaattgtact actgctcttg tataagaaga
     1021 aacagacaca aatacagcta cgggcgtcag gcaaaccgaa cgctgaagaa tttactggta
     1081 ccgtcattag attctgttcc ggcatgggta tatggtgtga cagggaaaat aatatctgaa
     1141 ttgtctgaac gttgagacta agagggccgg aggccaggtg aatggccacc gggatattta
     1201 tatcagagta tattggcgat tgattttgcc agctggtctt ccagatgagg tttag
//
LOCUS       DAZFXT010000337         1248 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.340, whole genome shotgun sequence.
ACCESSION   DAZFXT010000337 DAZFXT010000000
VERSION     DAZFXT010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.340"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..>1248
                     /locus_tag="ACHKW0_005237"
     CDS             126..>1248
                     /locus_tag="ACHKW0_005237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO7477862.1"
                     /translation="MSKVFICAAIPDEQAIKEEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIICEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCKPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQHTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KNEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPVALNKYITEY
                     LTTKGVFEDEGTNQSATDTLSSPVPETDAVETAIPDNEKT"
BASE COUNT          378 a          296 c          301 g          273 t
ORIGIN      
        1 caggataata acgaaatccc ggcaggtctt taaaacagtt ccgtaataaa catccgattt
       61 cattcttata tgccagcaat ggcagggatt tgttcaccct taaatctgta atgaggtaaa
      121 acaaaatgag taaagtcttt atttgcgccg ccattccgga cgaacaggca ataaaggaag
      181 aaggtgcagt cgctgtagcc acagctattg aagctggcga cgaacgccgt gctcgagcaa
      241 aatttcactg gcaattcctg gaacattatc cggctgctca ggactgcgct tataaattta
      301 ttatctgcga ggataaacct ggcatacccc gccctgccct cgattcatgg gatgctgaat
      361 atatgcagga aaaccgctgg gatgaggagt ctgcttcctt tgttccggtt gagactgaat
      421 cagatccgat gaacgtcact tttgacaagc tggcccctga agtacagaac gctgtcatgg
      481 ttaagttcga cacatgtgaa aacatcaccg ttgatatggt tattagcgca caggaattgt
      541 tgcaggaaga catggcaaca ttcgacggac atatcgttga agcgttgatg aaaatgccag
      601 aagttaacgc catgtatccg gagcttaagc tgcatgccat cgggtgggtt aagcataaat
      661 gtaagcctgg tgccaaatgg cccgaaattc aggcagagat gcgcatctgg aaaaaacgtc
      721 gcgaaggtga acgcaaggaa accggaaaat acacgtctgt tgttgatctc gcccgcgcca
      781 gaaccaatca acagcacact gaaaattcaa caggaaaaat cagcccggtc attgctgcca
      841 ttcatcgcga atacaagcag acatggaaaa cactggatga cgaactggcc tacgctctct
      901 ggcctggtga tgtggatgcc ggaaacattg acggcagcat ccatcgctgg gcaaaaaatg
      961 aagttatcga caacgaccgc gaagactgga agcgtatctc ggcatcaatg cgcaaacagc
     1021 ctgatgccct tcgctacgac cgccagacta tttttggcct tgtccgtgaa cgtccgatcg
     1081 acattcacaa agaccctgtg gcactgaaca aatacattac tgaatacctg actacaaagg
     1141 gcgtgtttga agatgaagga acaaatcaga gcgcaactga tactctctcg tcgccagtac
     1201 cagaaactga tgcagtggaa acggcaattc cggacaacga aaaaaccg
//
LOCUS       DAZFXT010000338         1247 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.341, whole genome shotgun sequence.
ACCESSION   DAZFXT010000338 DAZFXT010000000
VERSION     DAZFXT010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1247
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.341"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..1247
                     /locus_tag="ACHKW0_005238"
     rRNA            <1..1247
                     /locus_tag="ACHKW0_005238"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          311 a          291 c          391 g          254 t
ORIGIN      
        1 ctgagaggat gaccagccac actggaactg agacacggtc cagactccta cgggaggcag
       61 cagtggggaa tattgcacaa tgggcgcaag cctgatgcag ccatgccgcg tgtatgaaga
      121 aggccttcgg gttgtaaagt actttcagcg gggaggaagg gagtaaagtt aatacctttg
      181 ctcattgacg ttacccgcag aagaagcacc ggctaactcc gtgccagcag ccgcggtaat
      241 acggagggtg caagcgttaa tcggaattac tgggcgtaaa gcgcacgcag gcggtttgtt
      301 aagtcagatg tgaaatcccc gggctcaacc tgggaactgc atctgatact ggcaagcttg
      361 agtctcgtag aggggggtag aattccaggt gtagcggtga aatgcgtaga gatctggagg
      421 aataccggtg gcgaaggcgg ccccctggac gaagactgac gctcaggtgc gaaagcgtgg
      481 ggagcaaaca ggattagata ccctggtagt ccacgccgta aacgatgtcg acttggaggt
      541 tgtgcccttg aggcgtggct tccggagcta acgcgttaag tcgaccgcct ggggagtacg
      601 gccgcaaggt taaaactcaa atgaattgac gggggcccgc acaagcggtg gagcatgtgg
      661 tttaattcga tgcaacgcga agaaccttac ctggtcttga catccacaga actttccaga
      721 gatggattgg tgccttcggg aactgtgaga caggtgctgc atggctgtcg tcagctcgtg
      781 ttgtgaaatg ttgggttaag tcccgcaacg agcgcaaccc ttatcctttg ttgccagcgg
      841 tccggccggg aactcaaagg agactgccag tgataaactg gaggaaggtg gggatgacgt
      901 caagtcatca tggcccttac gaccagggct acacacgtgc tacaatggcg catacaaaga
      961 gaagcgacct cgcgagagca agcggacctc ataaagtgcg tcgtagtccg gattggagtc
     1021 tgcaactcga ctccatgaag tcggaatcgc tagtaatcgt ggatcagaat gccacggtga
     1081 atacgttccc gggccttgta cacaccgccc gtcacaccat gggagtgggt tgcaaaagaa
     1141 gtaggtagct taaccttcgg gagggcgctt accactttgt gattcatgac tggggtgaag
     1201 tcgtaacaag gtaaccgtag gggaacctgc ggttggatca cctcctt
//
LOCUS       DAZFXT010000339         1246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.342, whole genome shotgun sequence.
ACCESSION   DAZFXT010000339 DAZFXT010000000
VERSION     DAZFXT010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.342"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..488)
                     /locus_tag="ACHKW0_005239"
     CDS             complement(<1..488)
                     /locus_tag="ACHKW0_005239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477863.1"
                     /translation="MGKGGGRAHTPREAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLMDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETGLGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVETTSKGDRNPTSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQ"
     gene            622..1149
                     /gene="sodC"
                     /locus_tag="ACHKW0_005240"
     CDS             622..1149
                     /gene="sodC"
                     /locus_tag="ACHKW0_005240"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC"
                     /protein_id="HFO7477864.1"
                     /translation="MKCKIIAAIAMLTAASCGYAAEQEVPMNLVSADGKEVSIGKITI
                     QETPYGLLFTPALHSLSEGIHGFHVHEKGNCAPALKDGKPVAALSAGGHFDPKNTGKH
                     LGPWSPDGHLGDLPALFVTHDGKANYPVLAPRLNSLKEIKGRSLMLHAGGDNHHDHPE
                     PLGGGGARMACGIIQ"
BASE COUNT          281 a          361 c          305 g          299 t
ORIGIN      
        1 tactgtgagg tggtcttgcc gttaatggtg acatcctttt ccgtcaccca gttaccgtta
       61 cgctgcaact gaatcagcag gcggacagac gtcgggttac ggtcaccctt tgaggtggtc
      121 tccaccagtg actgcacccc gaaggtaacc cgcaggcggt caatgttcgc ggacgtaatg
      181 gtgcgcgtca ccggctttgc cttcgtcact tccacgccca gtccggtttc agctccggag
      241 gactcaaagc cttccggtgg tgtctgctcc tgctccccgg cacgccagac cgcagtcaca
      301 ccgtgtatca cgggattacc gtccgtgtcc atcagcgggg ttttgttcac caggatactc
      361 tgcagcccct tcaccgggcc ttctatcggt ccctcaccaa tcgcatcaat cacactcatc
      421 atctgcgtgg atttgagatt atccttcgcc tctcgaggcg tgtgcgccct gccgccacct
      481 ttgcccataa tgttcctctc aattggtatt attaatcgca gtgataggat attgcacagc
      541 tattgcgcga tatcatcaga acgctgtttg ttaccctgta accagcaagc tcagtctgtt
      601 aacggaatta atgagggttt tatgaaatgt aaaatcattg ctgccattgc catgctgaca
      661 gcagcatcat gcggatacgc agcagaacag gaagtcccaa tgaaccttgt cagtgctgac
      721 ggaaaagaag tcagcattgg aaaaataacc attcaggaga ccccctacgg tctgctgttc
      781 acaccagccc ttcactctct gtctgaaggc attcatggtt ttcatgtgca cgaaaaagga
      841 aattgcgccc cggcactgaa agacggaaaa ccggtcgcag cattatcggc tggcggtcac
      901 tttgacccga aaaacaccgg caaacatctt ggcccctggt ctccggatgg acacctgggc
      961 gacctccctg cgctgttcgt gacgcatgac ggaaaagcga actacccggt cctggccccg
     1021 agactgaact cattaaaaga gattaaaggg cgttctctca tgcttcatgc tggcggtgat
     1081 aaccatcatg accatccgga gcccctgggc ggtggtggtg cgagaatggc ctgcggcatc
     1141 attcaataat cagtcaggta aggggcgggc cccttacctt tattcctcag gacgataaat
     1201 cctttctccc tgaaaagaac ggcacatcct ccctctctga gttaat
//
LOCUS       DAZFXT010000340         1238 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.343, whole genome shotgun sequence.
ACCESSION   DAZFXT010000340 DAZFXT010000000
VERSION     DAZFXT010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1238)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1238)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1238
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.343"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(45..989)
                     /gene="leuO"
                     /locus_tag="ACHKW0_005241"
     CDS             complement(45..989)
                     /gene="leuO"
                     /locus_tag="ACHKW0_005241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HFO7477865.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT          375 a          285 c          248 g          330 t
ORIGIN      
        1 acagaataaa ccagacattc atgcctgacc tattctgcat tcagttagcg tttgcaaatt
       61 gagactaatt gctcttccat ccactgatgg cctttatcgc gcccggcagc ttcatgccag
      121 gagagataac aggttctgct attttgtttt aacggcagcg gtaatacctg taattctaag
      181 gattcagcga actcttcagc cagccaacgc ggcgcaatag cgaccaaatg cgtttgcgac
      241 accacgctaa gtacgctcat cattgccatg ccctgatacg cgatactggc ttgcttatct
      301 accgtgtcat accaaggttg actaaatgac gcgaaacgat cgagcgaaac cgccgcatgt
      361 tgttcgttat aaacatcatg tttcagtaac gggcccttaa ttgttggatg atttttgctg
      421 gctaccagca ccatttcatc tttaaataat ggcacgctgg taaattcagg acgatggaag
      481 tcttcataac taatcacaaa ctccgtttcc tgataacgca gctgatgttc agtgttctga
      541 tttaatgaag acttgaacat aacatgtata tttggcgcaa tctgctcaat gtgattataa
      601 atctgcgagg tcagaatgct gtctaacggg ctgcaaacac aaagatgaaa tacacgttca
      661 ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt gtactagttg caatgcctga
      721 cgaactgagc caaaaagttg aaatgcgcga gcagtcggtt gaataccacg gccataacga
      781 acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa cagcgttact gaccgcaggt
      841 tgcgacatac ccagaacatg agcggcacgg gtaatgtttt gctcctgcat cacggcatcg
      901 aaaacggtta ataagttgag atcgaccatg cgtagctgtg gcttgcttaa ctccgccgtc
      961 tctgaatgat ctgtttgtac ctctggcata cttaactcca ctgtcacact taactccctt
     1021 tcccttattg gaatgcagag aaataatcct gaagaatatg atttatcatg catataaaat
     1081 aagaaaaagc aaaatgagta aaattcggaa aacataaaga tacgggttct gataaaaaca
     1141 gaatccattg ccgtggaatg agtcatttac cacagcataa taatccataa tatttttgtt
     1201 gcgaaaacaa tctaatcata actaccgcga atactcaa
//
LOCUS       DAZFXT010000341         1201 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.344, whole genome shotgun sequence.
ACCESSION   DAZFXT010000341 DAZFXT010000000
VERSION     DAZFXT010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1201
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.344"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            2..580
                     /gene="tccP2"
                     /locus_tag="ACHKW0_005242"
                     /pseudo
     CDS             2..580
                     /gene="tccP2"
                     /locus_tag="ACHKW0_005242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_107686092.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tir-cytoskeleton coupling protein TccP2"
     gene            complement(726..995)
                     /locus_tag="ACHKW0_005243"
     CDS             complement(726..995)
                     /locus_tag="ACHKW0_005243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7477866.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQARVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            complement(997..>1201)
                     /locus_tag="ACHKW0_005244"
     CDS             complement(997..>1201)
                     /locus_tag="ACHKW0_005244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7477867.1"
                     /translation="NIIETNSHGWFPDTDGALITGLTFLDPKDATRVQGFFQHLQVRF
                     GDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          305 a          349 c          233 g          314 t
ORIGIN      
        1 cattaattct tttttttcca gccttcctcg cagtatatca agtgctatac gcagcagtac
       61 cttcactgtc tcgcagcaca aaagcacacc taacacggta aaaaccagct cacctttttc
      121 tcccagcaac agtcctgcca gtgcaacaac tatttttaag gtaaaaaatt cttatacaga
      181 atcaggactt caacgtccca catcttatac tcagtcaagt atagaaaaaa acgctttaca
      241 tcggcctcta cctgatgtgg ctcagcgtct ggtgcagcat cttgcagagc atggcattca
      301 accagcccgg aatatggctg aacatattcc tccggcacct aactggcctg cgccaccacc
      361 gccagtacaa aatgaacaat caagacctct gcctgatgtg gctcagcgtc tgatgcagca
      421 tcttgcagag catggcattc aaccagcccg gaatatggct gaatatattc ctccggcacc
      481 taactggcct gcgccaacgc cgccggtaca aaatgaacaa tcaagacctc tgcctgatgt
      541 ggctcagcgt ctgatgcagc atcttgcaga gcatggtatt aatacatcta agcgctcgtg
      601 attataatat aattacctgt attagctctg acctgacagt taccggttat ttatacaggt
      661 atctgtcaga ttacatctaa ccaacaaaaa ccggagccgg actccggttt ttgtgaagct
      721 gtcggctatt tcattccgcc aatattttcc cacctcccgt cagcacgcag gatttgcagc
      781 ggtcttacca cacactgtat ctgcttttta tccgcatcca gtatcaccac ctgcgtgatt
      841 accctggcct gctccgggat aatgccattc tcatcggact ccagaatgtc tgccggtccc
      901 agtcgcagct gtgctgtaag cgactgcacg tgttcacggc catcatgctt tccgcaacca
      961 cacagacgct gcataagttt ttttagtata ttcatgtcat tctcctgttc tgcctgtatc
     1021 actgcccact tcatcaagcc ccttaacatc ctgccacggc ccgtcaccaa acctgacctg
     1081 caaatgctga aaaaaaccct gaacccgtgt ggcatctttg gggtcaagaa aggtcagtcc
     1141 ggtgatgagc gcaccatctg tatccgggaa ccagccatgg ctgtttgtct caataatgtt
     1201 t
//
LOCUS       DAZFXT010000342         1195 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.345, whole genome shotgun sequence.
ACCESSION   DAZFXT010000342 DAZFXT010000000
VERSION     DAZFXT010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.345"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(76..873)
                     /gene="mtfA"
                     /locus_tag="ACHKW0_005245"
     CDS             complement(76..873)
                     /gene="mtfA"
                     /locus_tag="ACHKW0_005245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HFO7477868.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHRTNDTDSFSATNVH"
     gene            967..1056
                     /locus_tag="ACHKW0_005246"
     tRNA            967..1056
                     /locus_tag="ACHKW0_005246"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1001..1003,aa:Ser,seq:cga)
     gene            complement(1084..1195)
                     /locus_tag="ACHKW0_005247"
                     /pseudo
     CDS             complement(1084..1195)
                     /locus_tag="ACHKW0_005247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105555.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          356 a          278 c          258 g          303 t
ORIGIN      
        1 ttagcggtac cttgtctgtc aacactaaat ttcataagtc atttcaattg gttaattaat
       61 ctgcaaagtt gttaattaat gaacattcgt cgccgaaaac gagtctgtat cattagtgcg
      121 atgcagtctc tgcaaaggat cttgttgata aaattggcag aaacgttgcc acaatgaagg
      181 gaaacgagga gcaaaaagtt ctggggcgct aaagaaatat tcagaaagta cggcaaaaca
      241 ttcagcagga tcactggcag cataagcatc aatgctcgcc gcattctcgc caaccaattc
      301 gatttcttcc tgaatgttgt tcattgcagc atgaagatcg tgttcccagc cagcaacctc
      361 acgcaacgga ataaagggaa ctccgctggc gcgatcgccg ttacgggtgt ccagcttatg
      421 agcgacttca tgaataatca ggttaaaacc agaagcatca aaagaatctt gtatatccaa
      481 ccagttcaaa acgataggcc cttgctgcca gctctgacct gactgaacaa tacgttggtt
      541 atgcaccaga ccgatatcgt cttcccattc atcatcgacc acaaatggcg caggataaat
      601 taagacttca tgaaaaccat ccagccattc cagtcctaac tccagaacgg gtaggcaaaa
      661 tagaagtgct atccggcagc ttcttaatga atccagttca aagccctgta aaggaacaag
      721 ccgcttttgc tgtaaaaaac gttcggcaag agtgactaat ttgctttgtt cctgttccgt
      781 cagacacgtt aaaaggggga tcgatagtgc ttcctgccag ggaagggcag tttgatgtgc
      841 tgattcttgt actttccagg gccacttaat catcgttttg ctcgcaaact cgtcacttga
      901 acaaaattgc acggacaggg actgttaaaa tgccaaattt cctggcatca tggcaaccat
      961 ctgaacggag agatgccgga gcggctgaac ggaccggtct cgaaaaccgg agtgggggca
     1021 actccaccgg gggttcaaat ccccctctct ccgccaaaat tcaatcactt atacatcatt
     1081 aagtcagtga caaaaatcac acttggaatt acttggaata ttttcttgga atattttcag
     1141 gtaacgggac atcaagtgtc ggtgaaactt taaccttcct gtcatagatt agcac
//
LOCUS       DAZFXT010000343         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.346, whole genome shotgun sequence.
ACCESSION   DAZFXT010000343 DAZFXT010000000
VERSION     DAZFXT010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.346"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            99..239
                     /locus_tag="ACHKW0_005248"
     CDS             99..239
                     /locus_tag="ACHKW0_005248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7477869.1"
                     /translation="MSQKSLTAITFCVTVILIIWMLHGSLCEIRMSFWGAEFAAFLQC
                     KQ"
     gene            complement(429..710)
                     /locus_tag="ACHKW0_005249"
     CDS             complement(429..710)
                     /locus_tag="ACHKW0_005249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477870.1"
                     /translation="MRCGLLMAICLCLVSFSGYAATGKSDAQIKKEIIKESIESYPGN
                     CACPYNHARNGSRCGGRSAYSRTGGYDVVCYESDVTDEMIRQWKQENAE"
BASE COUNT          361 a          276 c          234 g          309 t
ORIGIN      
        1 ttaggatagc caaccatcag atgctgcatt tgcatagtgc aatctcccct tacttatccg
       61 gaggcaattg caatatctct gtgaacttac acagcgctat gtcgcaaaaa tcgcttacgg
      121 ccatcacgtt ctgcgtgacg gtaatcctca tcatctggat gctgcacggt tcgctgtgtg
      181 aaatacggat gagcttctgg ggagcggagt ttgcggcgtt cttacagtgt aagcagtaag
      241 gaaaccgcga cggggaggca actccccgtc aatcggttgc tagggtaagg ccgataaggc
      301 accctatctc ataggcatga ataacaaccc cgcagcgtaa aaactgcggg gtttcttttt
      361 ggaactctca ctagctagtg agccgttaac tgcacataat gctcgcacaa caaatcagtt
      421 aaaagagatt actcagcatt ctcctgcttc cattgccgga tcatttcatc ggtaacatca
      481 ctttcataac ataccacgtc ataccctcca gtacggctat atgcactgcg tccaccacat
      541 ctactaccat tccttgcatg attatacgga cacgcacaat tgcctggata agattcaatg
      601 gattctttaa ttatttcttt cttgatctga gcatctgact ttcctgttgc tgcataccca
      661 ctaaaagata ccaaacaaag gcagattgcc attaataaac cacatcgcat accgatacac
      721 tctattacta accaatcata gcccacatca taaaatcgag atgaggagta caacaccttt
      781 aacaagaagg tgccatcacg ataatatgcc gtgcaattca tcaaaaaaaa gatctatatc
      841 aaagagatag agttattatc agtgctgcag acatcagcat tcgaggcagc agaaagacag
      901 taaatctaac gaatttgtct ttttctcagg atgttgtcag attaaaaaag atagagtagc
      961 gcagacacct tttggtggat ggtaggagga aaaatgaagt tgagtgggaa gatcccagaa
     1021 aaaactttgg tttaaccaag gccctagttc aatatgcgaa atgcagatga gttgcaggag
     1081 agttttgctt cacccttaga gtttaaacag acgtgtacta tcattatttc tgcatatcgc
     1141 cacaccatcg ccactttacc gccatttaac aaaatacaaa
//
LOCUS       DAZFXT010000344         1180 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.347, whole genome shotgun sequence.
ACCESSION   DAZFXT010000344 DAZFXT010000000
VERSION     DAZFXT010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.347"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            79..1180
                     /locus_tag="ACHKW0_005250"
                     /pseudo
     CDS             79..1180
                     /locus_tag="ACHKW0_005250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc26 family transposase"
BASE COUNT          423 a          166 c          278 g          313 t
ORIGIN      
        1 ttttcatctt ggctttaacg cctacaggtg atcaaaattt aacctttgga ataactaaaa
       61 agataaaaaa ggacccaggt gagtattcaa agtttgcttg attatatttc agtgatccct
      121 gatatacgac aacaaggaaa ggttaaacat aaattatctg ctattttgtt tctcaccgta
      181 tgtgcagtaa ttgcaggtgc cgatgagtgg caggaaattg aagattttgg acatgaaaga
      241 cttgaatggc taaagaaata tggtgatttt gataatggca ttccggtcga tgacaccatt
      301 gcacgcgttg tgagtaacat tgacagtttg gcctttgaaa agatgtttat tgaatggatg
      361 caggagtgcc atgaaatcac tgatggtgaa attatagcaa tagatggaaa gaccataaga
      421 ggctcctttg ataagggaaa aagaaaagga gcaatccata tggtgagtgc attctcgaac
      481 gaaaatggtg ttgtactggg gcaggtgaaa acggaagcca aaagtaatga gattacagcc
      541 attccagagt tgcttaacct actgtattta aagaaaaatt tgataaccat tgatgctatg
      601 ggctgtcaga aagatatcgc ttcgaagatc aaagataaaa aagcagatta tcttctggca
      661 gtaaaaggca atcaggggaa attacatcat gcattcgagg aaaaatttcc tgtaaatgtg
      721 ttttctaatt ataaaggcga ttcgtttagt acgcaggaga taagtcatgg aagaaaagaa
      781 acacgtttgc atattgtcag taacgtaacg cctgaacttt tgtgattttg aattcgaatg
      841 gaagggatta aaaaagcttt gtgtagcatt gtcattcagg cagaagaaag aagataaatc
      901 agcagaaggt gtaagcatcc gatattatat ttcatcaaag gatatggatg ctaaagaatt
      961 tgcacatgct atcagagcgc actggctgat cgagcacagt cttcattggg tgttagatgt
     1021 aaaaatgaat gaagatgcca gccggataag aagaggaaac gcagcctaaa taatatctgg
     1081 aataaagaag atggcactga atttattaag agattgcaaa gacattaagg gtgaagagga
     1141 aaagaaagaa ggctgcgtta aacacaggga aagatcatca
//
LOCUS       DAZFXT010000345         1128 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.348, whole genome shotgun sequence.
ACCESSION   DAZFXT010000345 DAZFXT010000000
VERSION     DAZFXT010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.348"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            114..>1128
                     /gene="yfdF"
                     /locus_tag="ACHKW0_005251"
     CDS             114..>1128
                     /gene="yfdF"
                     /locus_tag="ACHKW0_005251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YfdF"
                     /protein_id="HFO7477871.1"
                     /translation="MLPSISINNTSATYPESINENNNDEVNGLVQELKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRFNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGNSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSEKARNASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTE
                     YQNE"
BASE COUNT          438 a          190 c          197 g          303 t
ORIGIN      
        1 tgcgctcata cactcaaatt aaagataggt tctaaataaa tgagcgtttt ttgatagtct
       61 atttcattag gtaatatata tttgtaacaa atcaatcaaa atggaataaa atcatgctac
      121 catctatttc aatcaacaat accagcgcaa cttacccaga atccattaat gaaaataaca
      181 atgatgaagt taatggatta gtacaagagc tcaaaaacct ttttaatggt aaggaaggaa
      241 taagcacctg tattaaacat ctacttgagc ttataaaaaa cgccatacga gtaaacgacg
      301 atccttatag atttaatatt aataattcct cagttactta tattgatatt ggctccaatg
      361 atacagacca tattactatt ggtatcgaca accaagaacc aatagaatta cctgcgaact
      421 ataaagacaa agaactcgtc cgtactatca ttaatgacaa catagttgag aagactcatg
      481 atatcaataa caaggaaatg atcttcagcg cattaaaaga aatatatgat ggagatcctg
      541 gttttatctt cgataagata tcacacaaac tcagacatac ggtaacggaa tttgatgaga
      601 gcgggaatag cgaaccaacg gacttattta cctggtacgg taaagataaa aaaggcgact
      661 ctctcgctat tgtaattaaa aataaaaacg gaaatgatta cttatctctc ggttactacg
      721 atcaggacga ctaccacatt caaagaggaa ttcgtattaa tggtgatagt ctcacccaat
      781 attgtagtga aaaagccagg aatgcttcag cgtggtttga aagcagtaaa gctatcatgg
      841 cagaatcatt tgcaacaggt tccgatcatc aggttgtaaa cgagctcaac ggggaaagac
      901 tgagagaacc taacgaagtt tttaaacgtt taggtcgagc aataagatat aattttcaag
      961 tggacgatgc aaaatttaga cgcgataatg taaaagaaat aatttctact ttattcgcta
     1021 acaaagttga cgttgaccat cctgaaaata aatacaaaga ctttaaggat ctcgaagata
     1081 aagttgaaaa aaggcttcaa aatcgccaga cggaatatca aaatgaaa
//
LOCUS       DAZFXT010000346         1115 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.349, whole genome shotgun sequence.
ACCESSION   DAZFXT010000346 DAZFXT010000000
VERSION     DAZFXT010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.349"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>1115
                     /locus_tag="ACHKW0_005252"
     CDS             <1..>1115
                     /locus_tag="ACHKW0_005252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HFO7477872.1"
                     /translation="ITRTITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQR
                     NGGWVTEKDITIKGKTTSQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSS
                     YTEIIDVKQGYPNTALVGVKVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGI
                     WDGTLKPAYSNNPAWCLWDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFG
                     GTEPRITCNAYLTTQRKAWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSN
                     VVMPDDGAPFRYSFSALKDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKM
                     DAFGCTSRGQAHRAGLWLIKTELLETQTVDFSVGAEGL"
BASE COUNT          259 a          291 c          352 g          213 t
ORIGIN      
        1 ataacccgga ccatcacgtc ggcaaacatt gaccgtctgc gcctgacctt cggtgtgcag
       61 gcactggtgg aaaccacctc aaagggggac aggaatccgt cggaagtccg cctgctggtt
      121 cagatacagc gtaatggtgg ctgggtgacg gaaaaagaca tcaccattaa gggcaaaacc
      181 acctcgcagt atctggcctc ggtggtggtg gataacctgc cgccgcgccc gtttaatatc
      241 cggatgcgca gaatgacgcc ggacagcacc acagaccagc tgcagaacaa aacgctctgg
      301 tcgtcataca ccgaaatcat cgatgtgaaa cagggctacc cgaacacggc actggtcggc
      361 gtaaaggtgg attcggagca gttcggcagc cagcaggtga gccgtaatta tcatcttcgc
      421 gggcgtattc tgcaggtgcc gtcgaactat aacccgcaga cgcggcaata cagcggtatc
      481 tgggacggaa cgttaaaacc ggcatacagc aacaacccgg cctggtgtct gtgggatatg
      541 ctgacccacc cgcgctacgg catggggaaa cgtcttggtg cggcggatgt ggataaatgg
      601 gcgctgtatg tcatcggcca gtactgcgac cagtcggtgc cggacggctt tggcggcacg
      661 gagccgcgca ttacctgtaa tgcgtacctg accacacagc gtaaggcgtg ggatgtgctc
      721 agtgatttct gctcggcgat gcgctgtatg ccggtatgga acgggcagac gctgacgttc
      781 gtgcaggacc gaccatcaga taaggtgtgg acctataacc gcagtaatgt ggtgatgccg
      841 gatgatggtg cgccgttccg ctacagcttc agcgccctga aggaccgcca taatgccgtt
      901 gaggtgaact ggattgaccc gaataacggc tgggagacgg cgacagagct tgttgaagat
      961 acgcaggcca ttgcccgtta cggtcgtaat gtcacgaaga tggatgcctt tggttgtacc
     1021 agtcgggggc aggcacaccg agccgggctg tggctgatta aaacggaact gctggaaacg
     1081 cagaccgtgg atttcagcgt gggcgcagaa gggct
//
LOCUS       DAZFXT010000347         1083 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.350, whole genome shotgun sequence.
ACCESSION   DAZFXT010000347 DAZFXT010000000
VERSION     DAZFXT010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1083)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1083)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1083
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.350"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(65..367)
                     /locus_tag="ACHKW0_005253"
     CDS             complement(65..367)
                     /locus_tag="ACHKW0_005253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477873.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            complement(364..990)
                     /locus_tag="ACHKW0_005254"
     CDS             complement(364..990)
                     /locus_tag="ACHKW0_005254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HFO7477874.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
BASE COUNT          308 a          242 c          191 g          342 t
ORIGIN      
        1 acaagataac aagataacaa gataacaaga taacaagata acaagataac aagataacaa
       61 ggtgttatct ttgttttact tctgctatca atcgtgatac ctctgttttt gatagtgttt
      121 taagtgcttc taccgccgcc ctgacaacat cagaacgatt cactctgcct ttacctgaaa
      181 ccatctcagc cgttataagg gcatcaatgt cttttaggtt ttcttcaaaa aaagaaatac
      241 tcactggttt tgctttgctt acaaccgatg atttatctga tgactttttg gtattatcaa
      301 ggccagccgt gccaccgtta ataaatgctg cctcgtcacg aggtgaagtg tttgctctat
      361 ttctcacaga ataacctctt tcactaacgc ttcgatttct tgctgggctt tatcattttg
      421 cgtttcaaaa attgtcattg attcggccca agcatctcga tgtgctttac gatcacatac
      481 ccgggagttt gccagatgca tttcagggta atccttaagt acttcagctg cctcattggc
      541 ttcattaaca aacatatttg tgggcgtcat gtttaaaacg agatatcccc gaacatcctc
      601 attaaaatct tttgctgctg taaaaacgga gcatgtatga ggcacaacat ccaggtccat
      661 ctgagatggg cgcaaaggag aaatgaatac atctgcggcc atcaatccac tacgcatttc
      721 tgcgctatca cggccagcac agtctgcaat aacaaaatca taggattttt cgtgttcctt
      781 taacatggct ttgatgttac ctgtcgcgcc tgtaaccgga atatgaggga gatcttcagg
      841 gcgattgttg taccaggtta atactgattt ctggtcatct gcgtcgacaa taatcactct
      901 attgcctagt gacatcagat acgcagctat agataccgcc agtgtacttt tacctacccc
      961 acccttttga ctacctaata ctattatcat tatagcccca tattaagttg ttaagttaac
     1021 aacttaatat tgccatctga acgcgaatca tgcaattatt tttgttatat taacaactta
     1081 ata
//
LOCUS       DAZFXT010000348         1082 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.351, whole genome shotgun sequence.
ACCESSION   DAZFXT010000348 DAZFXT010000000
VERSION     DAZFXT010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.351"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(1..1082)
                     /locus_tag="ACHKW0_005255"
                     /pseudo
     CDS             complement(1..1082)
                     /locus_tag="ACHKW0_005255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309571.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
BASE COUNT          217 a          349 c          253 g          263 t
ORIGIN      
        1 ttgtgcctgc gcggccatct tctcctcaaa tgtgcgctgt aaatccgcca gcaccgtggc
       61 gaattcttct cccagtgcac gaataatgga cagttcccgt tccgtcattt tcgcagtatc
      121 cccctcaaca tcgctttcac cgcaccatgc tctgtttcac tgattgcctt attaccgtca
      181 gatgcgccgt caggcagttg ggctgaaact cttttcccgg acgacgcaaa cggatcttca
      241 cgggcatcac gacgggacag cgcctccaga ctgtagttct gctgctgaag atacagtgca
      301 tcacctcccg caaggggcgg caggttctca cgtttacggg cctcattggg cgtgagaagc
      361 gtatttttca ccgactcacc cagcgttttc atgcgccgtt cgctgtccat tctcagcagc
      421 gtggtgacgt caaactccgt actctcgttt tcccccgttt ccagcgcctc atccagtaac
      481 agttcaatgg actcaatcag cgtctgcaga cactgggaat aatactgctg ctccagcgcc
      541 tccacgttat cactggaggg aggttgtccc acaccaatct tgtaggccgg gacacggaac
      601 accgaacaga caatttcagc cgtcattttc agttgttcca ccgtctgcgc atccaccggt
      661 gaaaacgtcg tggggttata tttcgccccg ttgctcagaa tcgccgtttt ccccgcattt
      721 tcgccggtat acccgctgtc ccagttgctc ttcagttttt tcgcattttc ttccgtaata
      781 ctgccgggga tctcaatcac cccggacggc ctgccgccat ttctgaaaaa agacgttgaa
      841 tttgcctgaa tatgatgccc ctgcgtggca gccagcccgg cagcatacac cggcggcagc
      901 cccacaagcg gatgaaaaaa acagttaaac cggtcgtgga tcacttcccg ggcaggcacc
      961 gtcaccgcct ccgtgatccc gcagttccgg tccggcgtga tgcgatagaa cacctcgccg
     1021 tcatccgcca ccagaggttc aacccggctc cagtccagaa tacgcagttc tttgatctgc
     1081 cc
//
LOCUS       DAZFXT010000349         1048 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.352, whole genome shotgun sequence.
ACCESSION   DAZFXT010000349 DAZFXT010000000
VERSION     DAZFXT010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.352"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..174
                     /locus_tag="ACHKW0_005256"
     CDS             <1..174
                     /locus_tag="ACHKW0_005256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477875.1"
                     /translation="YCGDGELLHHIPEQLSKRERYTDKWQRRTHSLWRHRAWRASAFT
                     GICNDLAAASTFV"
     gene            138..713
                     /locus_tag="ACHKW0_005257"
     CDS             138..713
                     /locus_tag="ACHKW0_005257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7477876.1"
                     /translation="MQRFGRRIDLRVKTGAEAIRALATQLPVFRQKLSDGWYQVRIAG
                     RDAGETELSSRLNEPLANGAVIHIVPRLAGAKSGGVFQAVLGAALIATAIWMPGISIA
                     FSDILFSMGAAMTLGGVAQMLAPKASTATTTSTDNGKQNTYFSSLDNMVAQGNVLPVL
                     YGEMRVGSRVISQEISTADEGDGGEIVVIGR"
     gene            774..>1048
                     /locus_tag="ACHKW0_005258"
     CDS             774..>1048
                     /locus_tag="ACHKW0_005258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477877.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPIEGPVDGLKS
                     VLLNSTPVLDSEENTNISGVTVVFRSGEQEQTPPEGFESSGSETVLGT"
BASE COUNT          236 a          250 c          342 g          220 t
ORIGIN      
        1 tactgtggtg acggcgagct gctgcaccat attcctgaac aactgagcaa acgagagagg
       61 tataccgaca aatggcagcg acgcacacac tccctctggc gtcaccgggc atggcgcgca
      121 tctgccttta cggggatttg caacgatttg gccgccgcat cgaccttcgt gtgaaaacgg
      181 gggctgaagc catccgcgca ctggccacac agctcccggt gtttcgtcag aaactgagcg
      241 acggctggta tcaggtacgg attgccgggc gtgatgcagg cgaaaccgaa ttatcatccc
      301 gtcttaatga gccgctggca aatggtgcag tgatccacat cgtgccgcgt cttgcgggag
      361 caaaaagtgg cggtgtgttt caggcggtgc tgggtgcggc gttgattgct acggcaatct
      421 ggatgccggg gatcagtatc gctttcagtg acattctctt ttcaatgggt gcggcgatga
      481 cgcttggcgg tgttgcacag atgctggcac cgaaagcgag taccgcaaca acgaccagca
      541 cggataacgg taagcagaac acgtatttct catcactgga taacatggtt gcccagggca
      601 atgttctgcc tgttctgtac ggtgaaatgc gtgtggggtc acgggtaatt tcgcaggaaa
      661 tcagcacggc agacgaaggg gatggtggtg aaatcgtggt gattggtcgc tgatgcagaa
      721 tgttttatgt gaaaccgcct ccgggcggtt ttgtcgttta tggagcgtga ggaatgggta
      781 aaggcagcag taaggggcat accccgcgcg aagcgaagga caacctgaag tccacgcagc
      841 tgctgagtgt gatcgatgcc atcagcgaag ggccgataga aggtccggtg gatggattaa
      901 aaagcgtgct gctgaacagt acgccggtgc tggacagtga ggagaatacc aatatctccg
      961 gcgtcacggt ggtgttccgg tcaggtgagc aggagcagac accgccggag gggtttgaat
     1021 cctccggctc cgagacggtg ctgggtac
//
LOCUS       DAZFXT010000350         1019 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.353, whole genome shotgun sequence.
ACCESSION   DAZFXT010000350 DAZFXT010000000
VERSION     DAZFXT010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.353"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..984
                     /locus_tag="ACHKW0_005259"
     CDS             <1..984
                     /locus_tag="ACHKW0_005259"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:1900727 - osmoregulated periplasmic glucan
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HFO7477878.1"
                     /translation="RQTGTDASRTINEKQKPVIMFLVVGETARSQNYALNGYSRGTND
                     FTKKYNELISFHNVQSCGTSTAISVPCMFSDMKRKEFNSRKAVNSENVLDILYRTGVN
                     LLWIENDGGCKGVCKRIPTINIEPSNSDNTLCKKNSCYDEVMLKNIDEYINNNSEDKL
                     IVFHLMGSHGPTYYLRYPEPHKYFKPTCDRSDIENCTHEQLINTYDNTIRYTDYIISK
                     LIDKLIEYKDEYDTVLLYVSDHGESLGENGLYLHGTPYNVAPAEQTHVPLITWMSPGF
                     VSSKKIDLNCLSEHALNRTVSHDNIFSSLLGLWNVNTSVYNKEDDIISSCR"
BASE COUNT          350 a          161 c          194 g          314 t
ORIGIN      
        1 cgtcagactg gaacggatgc atcccgcaca ataaatgaaa aacaaaagcc ggtaattatg
       61 tttttagttg tcggggagac agcaagaagt caaaactatg ctctgaatgg atattctcgt
      121 ggtaccaatg attttacaaa gaaatataat gaacttatat cctttcataa tgtgcagtcg
      181 tgtggcacat ccacagccat atctgttcca tgcatgtttt cagatatgaa gcgaaaagag
      241 ttcaattccc gcaaagcggt aaattcggaa aatgtactgg atattctgta cagaacgggg
      301 gtaaatttac tctggattga aaatgacggg ggatgtaaag gtgtatgtaa gcgaatccct
      361 acaattaata ttgagccatc aaattcagat aatacattat gcaagaaaaa ctcatgttat
      421 gatgaggtta tgctaaaaaa tattgatgag tatataaata ataactcaga ggataaactt
      481 atcgtatttc atttgatggg tagtcatggt cctacctact atctccgtta tcctgagcca
      541 cataaatatt ttaaaccaac atgtgatcgt agtgatatcg aaaattgtac gcatgagcaa
      601 ttgattaata cctatgataa tacaattcgc tatactgatt atataatatc aaaattaatt
      661 gacaagctta tcgaatataa agatgagtat gatactgttt tattatatgt ctctgaccat
      721 ggagaatctc tgggagaaaa tggattatac ttacatggaa caccatataa tgttgctcca
      781 gctgagcaaa cgcatgtgcc tcttattacc tggatgtctc cggggttcgt ttcctctaaa
      841 aaaattgatt tgaattgttt gtcagaacat gctctaaaca gaacagtaag tcatgataat
      901 atattttcat cgttactggg gttatggaat gtaaatactt cagtttataa taaagaagat
      961 gatattatat cctcctgcag atagatactg acatttttct gaggataaga ggtgaatac
//
LOCUS       DAZFXT010000351         1008 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.354, whole genome shotgun sequence.
ACCESSION   DAZFXT010000351 DAZFXT010000000
VERSION     DAZFXT010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.354"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(145..>1008)
                     /gene="cynX"
                     /locus_tag="ACHKW0_005260"
     CDS             complement(145..>1008)
                     /gene="cynX"
                     /locus_tag="ACHKW0_005260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HFO7477879.1"
                     /translation="GGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAA
                     ITPWLVQHSESWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAW
                     TLGVYFGLINGGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARH
                     QDRRKLLMLALVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSVQP
                     AIAGKLVAFMQGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAP
                     ARFPQLWVKEA"
BASE COUNT          283 a          312 c          248 g          165 t
ORIGIN      
        1 caatattatt tcagagatta caaagttgaa tcgtcagttt aaattataaa aattgcctga
       61 tacgcttcgc ttatcaggcc tacaaattca gtgatctacg taggccgcat ccggcgtgaa
      121 caaagcgcag aaacaagagt cgcatcatgc ctctttgacc cacagctgcg gaaaacgtgc
      181 tggtgcaaaa cgcagggtta taatcatcag cccaacgacg cacagcgcat gaaatgccca
      241 gtccatcagg taattgccgc tgatactacg cagcacgcca gaaaaccacg gggcaagccc
      301 ggcgatgata aaaccgattc cctgcataaa cgccaccagc ttgccagcaa tagccggttg
      361 cacagagtga tcgagcgcca gcagcaaaca gagcggaaac gcgccgccca gacctaaccc
      421 acacaccatc gcccacaata ccggcaattg cagcggcaac cagataaagc cgcagaaccc
      481 caccagttgt aacaccagcg ccagcattaa cagtttgcgc cgatcctgat ggcgagccat
      541 agcaggcatc agcaaagctc ctgcggcttg cccaagcgtc atcaatgcca gtaaggaacc
      601 gctgtactgc gcgctggcac caatctcaat atagaaagcg ggtaaccagg caatcaggct
      661 ggcgtaaccg ccgttaatca gaccgaagta aacacccagc gtccacgcgc ggggagtgaa
      721 taccacgcga accggagtgg ttgttgtctt gtgggaagag gcgacctcgc gggcgctttg
      781 ccaccaccag gcaaagagcg caacaacggc aggcagcgcc caccaggcga gtgtttgata
      841 ccaggattcg ctatgttgaa ctaaccaggg cgttatggcg gcaccaagtc caccgccgcc
      901 catcagagcc gcagaccaca gccccatcac cagtggcgtg cgctgctgaa accgccgttt
      961 aatcaccgaa ggcatcaccg cctgaatgat gccgatcccc accccacc
//
LOCUS       DAZFXT010000352         1004 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.355, whole genome shotgun sequence.
ACCESSION   DAZFXT010000352 DAZFXT010000000
VERSION     DAZFXT010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1004
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.355"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..833)
                     /locus_tag="ACHKW0_005261"
     CDS             complement(<1..833)
                     /locus_tag="ACHKW0_005261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HFO7477880.1"
                     /translation="MATLRELIIKISANSQSFQSEIQRASRMGSEYYRTLQNGGRQAA
                     AAAREQRRALAELHSQLTEIRASAVGMTGAFAGAFATGHLISLADEWSSVNARLKQAS
                     QSSDEFSSSQKVLMDISQRTGTAFSDNAALFARSAASMREYGYSADDVLKVTEAISTG
                     LKISGASTAEAGSVITQFSQALAQGVLRGEEFNSVNESGDRIVRALAAGMGVARKDLK
                     AMADDGKLTADKVVPALISQLGILRDEYAAMPETVSSSITKVENAFMAWVGGANEASG
                     VT"
     gene            complement(885..>1004)
                     /locus_tag="ACHKW0_005262"
     CDS             complement(885..>1004)
                     /locus_tag="ACHKW0_005262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477881.1"
                     /translation="QGMNVALKDAMLCWGEDEDKPEVDPFAALEDALSLAAMS"
BASE COUNT          217 a          335 c          229 g          223 t
ORIGIN      
        1 gtcaccccgc tggcctcatt cgcaccgccc acccaggcca taaaggcgtt ttccaccttc
       61 gtgatactac tggaaaccgt ttccggcatg gccgcatatt catcacgtaa tatccccagc
      121 tggctgatta acgcggggac cactttatcc gctgtcagtt ttccgtcatc cgccattgcc
      181 ttcagatctt tacgggccac gcccataccc gcagccagtg cacgtacgat ccggtcacca
      241 ctttcattga ccgaattaaa ctcctcacca cgcaatacac cctgcgccag cgcctgactg
      301 aactgggtga tcaccgagcc cgcctctgcc gtactggcac cggagatttt cagccctgtc
      361 gaaatggcct ccgtcacctt cagcacatca tcagcactgt aaccatattc acgcattgag
      421 gcggctgagc gggcaaacag ggccgcatta tccgaaaatg cggtgcctgt ccgctggctg
      481 atatccatca gcactttctg tgatgacgaa aattcatcag atgactgcga cgcctgtttc
      541 agacgggcat tcacggaact ccactcatcc gccagtgaaa tcaggtgtcc ggtggcaaag
      601 gcaccggcaa acgcaccggt cattccgaca gccgaagcgc ggatttccgt caactggctg
      661 tgcagctcag ccagagcccg gcgctgctcc ctggctgccg cagcagcctg acgtccgcca
      721 ttctgcaggg tccggtaata ttcactgccc atacgggacg cccgctggat ctccgactgg
      781 aatgactgtg aatttgccga aattttgata atcagttcac gtaacgtcgc cattcacctt
      841 tctccgggca aaaaaaacct gccacagcag gttttcatca ttatttatga cattgctgca
      901 aggctcagcg cgtcttccag cgccgcaaac ggatccacct ccggcttatc ctcatcctcg
      961 ccccagcaga gcatggcgtc cttcagtgca acattcatcc cctg
//
LOCUS       DAZFXT010000353         1001 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.356, whole genome shotgun sequence.
ACCESSION   DAZFXT010000353 DAZFXT010000000
VERSION     DAZFXT010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.356"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..998
                     /locus_tag="ACHKW0_005263"
     CDS             <1..998
                     /locus_tag="ACHKW0_005263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HFO7477882.1"
                     /translation="ALGLTGCDDKKAETETPPPANSQPAAPAPEAPVAKAEAKPETPA
                     QPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQGPTGEERTVYGIYG
                     ISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTINEMDKYYTQENYKD
                     DAFAKGKTLHQTFLKNLEAFEPVAESYHAAIQEINDKRQLAELKNIEEREGKTFHYYS
                     LAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKGGMNFSFINSAGQYQ
                     LEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT          317 a          213 c          250 g          221 t
ORIGIN      
        1 tggccctcgg tctgacgggt tgtgatgata aaaaagccga aacagaaacg cccccgcctg
       61 ccaatagtca acctgccgca ccagctcctg aagctcccgt tgcaaaagca gaagctaaac
      121 ctgaaacacc tgcgcaaccg gtggtcgatg aacaagcggt tttcgacgaa aaaatggatg
      181 tctatatcaa gtgctataac aagttacaga tccctgtaca gagcagtctg gcgcgttatg
      241 ctgactggct gaaagatttt aaacaggggc ctaccggtga agagcgtact gtttatggca
      301 tctacggcat tagtgaatcc aacctcgctg agtgtgaaaa aggcgtaaaa agtgctgtgg
      361 cgttaacgcc tgcgctgcaa ccaattgatg gcgtggcggt gagttatatc gatgctgccg
      421 tggcgttggg taataccatt aacgaaatgg ataaatatta cacccaggaa aattataaag
      481 acgatgcgtt tgcgaaaggg aaaacgctgc accagacatt cttaaaaaat ctggaagcct
      541 ttgaacctgt agcagaatct tatcatgcgg cgattcagga aattaacgat aagcgccagc
      601 ttgcggaact gaaaaatatt gaagaaagag aagggaaaac attccactac tactctctgg
      661 cagtcatgat ttcagcgaaa caaattaata acctgatatc gcaagagaag tttgatgttg
      721 atgcggcaat gaagaaagtg tctgaactgg aaacgctagt ggcgcaggcc aaagaagcgg
      781 ataaaggcgg catgaatttc tcgtttatta attcggcagg ccagtatcaa ctcgaggcta
      841 aaaaatacgt tcgccgcatc agagataaag tcccgtactc tgactgggat aaagagcaac
      901 ttcaggacgc aaactcaagc tggatggtcg aagactcttt cccgagagca ttacgcgagt
      961 acaacgaaat ggttgatgac tataatagcc tgcgttaaac g
//
LOCUS       DAZFXT010000354          995 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.357, whole genome shotgun sequence.
ACCESSION   DAZFXT010000354 DAZFXT010000000
VERSION     DAZFXT010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..995
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.357"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..510)
                     /locus_tag="ACHKW0_005264"
     CDS             complement(<1..510)
                     /locus_tag="ACHKW0_005264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HFO7477883.1"
                     /translation="MSIKHYDVVRAASPSDLAEKLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVTTPVLVKPSDGEGTVISATRDPEYYFVVVLAGQSNSMAYGEGLPLPETY
                     DRPDPRIKQLARRSTVTPGGVACKYNDIIPADHCLHDVQDMSRLNHPKADLSKGQYGT
                     VGQGLHIAKK"
     gene            487..629
                     /locus_tag="ACHKW0_005265"
                     /pseudo
     CDS             487..629
                     /locus_tag="ACHKW0_005265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(824..937)
                     /locus_tag="ACHKW0_005266"
     CDS             complement(824..937)
                     /locus_tag="ACHKW0_005266"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7477884.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIVVLLWPVIKRNSLHNA"
BASE COUNT          248 a          288 c          241 g          218 t
ORIGIN      
        1 ttttttggcg atatgcagcc cttgccccac ggttccgtac tgcccctttg acaggtccgc
       61 tttcggatgg ttaaggcggc tcatgtcctg cacatcatgc agacaatggt ccgccggaat
      121 gatgtcgtta tatttacagg cgacaccgcc cggtgtcacc gtactgcgac gcgccagctg
      181 cttaatgcgc gggtccggac ggtcatatgt ctccggcagc ggaaggcctt caccatatgc
      241 catgctgttt gactgccccg ccagaaccac aacaaagtaa tactccgggt ctctggtggc
      301 gctgattact gtgccttctc catccgacgg cttcaccaac acaggtgtgg tgacatcacc
      361 ttccgccgca atggcctgca tcagagtata aggcgtgatg gccaccgggc tgccaaatgg
      421 ctgccacccc tccttcagtt tttgtgtcag cttttccgca aggtctgacg gcgacgccgc
      481 cctgacaaca tcataatgtt taatcgacat cgaatttctc ccgtgtagag gaacagagtt
      541 aaaaagccgg aagcggaatc aaatcacagg atggccatct gccagtggct ggtcgtaaaa
      601 aaaggccacg ccatgcgcag ccggaaataa agggataacg atgatagttt gagaaaaaca
      661 gaaacaacac ttttgtggca aagcatggtg ccgggtgcct cccggtgaat tcagtatcag
      721 cacctgaatc cgcgattatc ccatatacct ggttgctgat cgcccctccg cacaggggga
      781 ttcaccatgc agaagtgttt ttaataaaca gcaaacaaaa aaatcaagca ttatgcaggc
      841 tgtttctttt tatcaccggc cacagcaata ccacaatgcc gcagaccagc accccatccg
      901 ccagcaccga catgattctg ctggtgaaat ccaccatcac caccagaaac agcaggagtg
      961 cagccacagc caggcgcagt tttaccgtca caggt
//
LOCUS       DAZFXT010000355          991 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.358, whole genome shotgun sequence.
ACCESSION   DAZFXT010000355 DAZFXT010000000
VERSION     DAZFXT010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.358"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..242)
                     /locus_tag="ACHKW0_005267"
     CDS             complement(<1..242)
                     /locus_tag="ACHKW0_005267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477885.1"
                     /translation="MAIKGLDQAIENLSRVRKNAIPAASAMAINRVATTAINQSSSQV
                     ARETKVRRKLVKERSRLKRATVRNPNARIIVNRGDLP"
     gene            complement(257..610)
                     /locus_tag="ACHKW0_005268"
     CDS             complement(257..610)
                     /locus_tag="ACHKW0_005268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001204533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HFO7477886.1"
                     /translation="MRDFQNAFDAALAGVDSTIVEVMGLCAQFTSGAQCGSEVQGVFD
                     DPESLGFAGSGVRIEGSCPSLFVRTDTVRAVRRGDTLTINGETFWVDRVSPDDGGSCY
                     LWLNRGQPPAVNRRR"
     gene            complement(603..986)
                     /gene="gpFI"
                     /locus_tag="ACHKW0_005269"
     CDS             complement(603..986)
                     /gene="gpFI"
                     /locus_tag="ACHKW0_005269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HFO7477887.1"
                     /translation="MATKEENQKRLRQLAGLLGREADMSGSAADIAQRVSEWEEELAA
                     SREGIMPGDESGPEQNHTDDGEQLHNTDATDDVKAVRVRQCLHVMGYCPETGRPVELT
                     YRGMRVLVPSPLATAMIQHGTAEHA"
BASE COUNT          216 a          327 c          226 g          222 t
ORIGIN      
        1 gggagatcac cgcggttaac gataattctg gcattcggat ttctgaccgt cgcccgtttc
       61 agtctggacc gttccttaac cagtttccgg cgaacctttg tctcccgggc aacctgtgat
      121 gaagactgat taatcgccgt tgtggccacg cggttaatgg ccattgctga agccgccgga
      181 atggcgtttt tacgaacccg gctcagattt tcaatcgcct gatcaagccc ttttatcgcc
      241 ataattcacc ctgcgtttat cgtcgccggt taactgccgg tggttgccca cggttgagcc
      301 agagataaca actgcccccg tcatccggag aaacacgatc cacccagaat gtctcaccat
      361 taatggtcag cgtgtcacca cgccgcacag cacgaaccgt atccgtccgt acaaataatg
      421 acgggcagct tccttcaata cggaccccgc taccggcaaa ccccagcgac tccggatcgt
      481 caaaaacccc ctgaacttcg ctgccacact gtgcccccga ggtgaactgc gcacagagcc
      541 ccatcacttc aacaatcgtg ctgtccaccc cggcaagggc tgcatcaaag gcattctgaa
      601 aatcacgcat gctcagccgt tccgtgctgt atcatggctg tcgccagtgg tgatggcacc
      661 agaacacgca tgccccggta cgtcagttca acgggacggc ctgtctccgg gcaatacccc
      721 atcacatgca ggcattgccg cacacggacc gctttaacat catccgtagc atcagtgttg
      781 tgcaactgct caccatcgtc tgtgtgattt tgctcaggcc cgctctcatc accaggcata
      841 atgccctccc gggaagcagc aagctcctct tcccactcag acacacgttg cgcaatatcc
      901 gcagcactcc ccgacatatc cgcctcgcgc cccagcaggc cagccagttg acgaagacgt
      961 ttctgatttt cttcttttgt tgccatatca g
//
LOCUS       DAZFXT010000356          968 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.359, whole genome shotgun sequence.
ACCESSION   DAZFXT010000356 DAZFXT010000000
VERSION     DAZFXT010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..968
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.359"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            85..870
                     /locus_tag="ACHKW0_005270"
     CDS             85..870
                     /locus_tag="ACHKW0_005270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477888.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          320 a          191 c          210 g          247 t
ORIGIN      
        1 ttgttctgat tcaagtggag tatataatta aaaatcgtta gctaaacatt accatgatta
       61 tgacaggcga gggagtgtcc aattatgaat tcaaaaaagc tttgttgcat atgtgtgtta
      121 ttctcgctgc ttgcaggatg tgcctctgaa tcttctattg atgaaaagaa gaaaaaagct
      181 caagtcacac aaagtaatat taataaaaac actccccagc aactgacaga caaagattta
      241 ttcggtaatg aaaccactct ggccgtatcc gaagaggata ttcaagctgc gcttgatgga
      301 gatgagttcc gcgttcccct taattctcct gtaatacttg ttcaatccgg caaccgcgca
      361 ccggaaacca ttatgcagga ggagatgcga aaatattata ctgtttccac attctctggt
      421 atcccggaca ggcaaaagcc tctgacttgt aacaaaaaca aagataaaaa tgagaacgag
      481 gatgttgcca gtgctgagaa tatgaactgg atgcaggcac tgcgttttgt ggctgcaaaa
      541 gggcatcaga aagcgattat tgttttccag gatacgttgc agacaggaaa atatgactct
      601 gcgctgaaat caacagtatg gtccgactat aaaaatgaaa aactcaccga cgctatctcc
      661 ctgcgctacc tggtacgttt cacgctggtg gatgtggcaa caggtgagtg ggctacctgg
      721 tcgccggtga attatgaata taaagtgctt ccaccattgc ccgacaagaa tgaagccagt
      781 acgactgata tgacagagca gcaaatcatg caacttaagc aaaagaccta taaagcgatg
      841 gtaaaagatt tggttaaccg ctatcaataa taaattatat ctgccgccag gaatttctgg
      901 cggcaataat acaaaattct ttgcataaga aatttttctt gacaaaaaca gtgcaagaaa
      961 ggacatgg
//
LOCUS       DAZFXT010000357          962 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.360, whole genome shotgun sequence.
ACCESSION   DAZFXT010000357 DAZFXT010000000
VERSION     DAZFXT010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.360"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..154)
                     /locus_tag="ACHKW0_005271"
     CDS             complement(<1..154)
                     /locus_tag="ACHKW0_005271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7477889.1"
                     /translation="MASISIRCPSCSATEGVVRNGKSTAGHQRYLCSPCRKTWQLQFT
                     YTASQPG"
     gene            complement(415..684)
                     /locus_tag="ACHKW0_005272"
     CDS             complement(415..684)
                     /locus_tag="ACHKW0_005272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7477890.1"
                     /translation="MNILKKLMQRLCGCGKHDDREHGELLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGRWENIGGMK"
     gene            complement(686..>962)
                     /locus_tag="ACHKW0_005273"
     CDS             complement(686..>962)
                     /locus_tag="ACHKW0_005273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7477891.1"
                     /translation="GATGPQGPKGDPGETQIRFRLGPGNIIETNSHGWFPDTDGALIT
                     GLTFLDPKDTTRVQGFFQHLQVRFGDGPWQDVKGLDEVGSDTGRTGE"
BASE COUNT          223 a          275 c          215 g          249 t
ORIGIN      
        1 taccgggctg agaggcggtg taagtgaact gtagttgcca tgttttacgg caaggagagc
       61 agagatagcg ctgatgtccg gcagtgcttt tgccgttacg caccacgcct tcagtagcgg
      121 agcaggaagg acatctgatg gaaatggaag ccacgcaagc accttaaaat caccatcata
      181 cactaaatca gtaagttggc agcattaccg agattgtttt gtcggaactg atacgctggc
      241 gctaataggt agtagggttc ttatgctaaa catacaacct ctttcttaat tctcgaacct
      301 gtttaggatt ctgtgtaaat tcaaaataaa cctgtctgaa cattcctaac aaacatccac
      361 cggacatgac aacaaaaaac cggagccgga ctccggtttt tgtgaagctg tcggctattt
      421 cattccgcca atattttccc acctcccgtc agcacgcagg atttgcagcg gtcttaccac
      481 gcactgtatc tgctttttat ccgcatccag tatcaccacc tgcgtgatta ccctgtcctg
      541 ctccgggata atgccattct catcggactc caggatgtct gccggcccca gtcgcagctg
      601 tgctgtaagt aactccccgt gttcacggtc atcatgcttt ccgcaaccac acagacgctg
      661 cataagtttt tttagtatat tcatgtcatt ctcctgttct gcctgtatca ctgcccactt
      721 catccagccc cttgacatcc tgccacggcc cgtcaccaaa cctgacctgc aaatgctgaa
      781 aaaaaccctg aacccgtgtg gtatctttgg ggtcaagaaa ggtcagtccg gtgatgagtg
      841 cgccatctgt atccgggaac cagccatggc tgtttgtctc aataatgttt cccggcccca
      901 gacggaaccg tatttgcgtc tcccccgggt cgcccttcgg cccctgaggt ccggtcgccc
      961 cc
//
LOCUS       DAZFXT010000358          960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.361, whole genome shotgun sequence.
ACCESSION   DAZFXT010000358 DAZFXT010000000
VERSION     DAZFXT010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.361"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>960
                     /locus_tag="ACHKW0_005274"
     CDS             <1..>960
                     /locus_tag="ACHKW0_005274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="terminase gpA endonuclease subunit"
                     /protein_id="HFO7477892.1"
                     /translation="QYLKFGDESTPFGLKWEKDSPESVFYLCEHHGCVIHQSELDQSN
                     GRWICENTGMWTRDGLTFFSARGDEIPPPRSITFHIWTAYSPFTTWVQIVYDWLDALK
                     DPNGLKTFVNTTLGETWEEAVGEKLDHQVLMDKVVHYTAAVPARVVYLTAGIDSQRNR
                     FEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYCHADGTEMTISRVCW
                     DTGGIDGEIVYQRSKKHGVFRVLPVKGASVYGKPVITMPKTRNQRGVYLCEVGTDTAK
                     EILYARMKADPTPADEATSYAIRFPDDPEIFSQTEAQQLVAEEL"
BASE COUNT          232 a          232 c          287 g          209 t
ORIGIN      
        1 gcagtatctg aaatttggcg atgagtccac gccttttggg cttaaatggg agaaggacag
       61 ccccgaaagt gttttctacc tctgtgaaca tcatggctgc gtgatccatc agtctgaact
      121 ggaccagagc aacgggcggt ggatctgtga aaacacgggc atgtggaccc gtgacggtct
      181 gacgtttttc agcgcccggg gtgatgaaat tccgccgccg cgctccatca cgttccatat
      241 ctggacggcg tacagtccgt ttaccacctg ggtacagata gtctatgact ggctggatgc
      301 actgaaagat cccaacggcc tgaaaacctt tgtgaacacc acgctgggcg agacctggga
      361 agaagccgtg ggcgaaaaac tcgatcacca ggtactgatg gataaggtcg tgcattacac
      421 ggcggcggta cctgcccggg tggtttatct gacggcgggc attgactcgc agcgaaaccg
      481 ttttgagatg tatgtctggg gatgggcacc gggagaggaa gcttttctgg tggataaaat
      541 catcattatg ggccgtcccg atgaggaaga gacgctgtta cgtgtggatg cggcgatcaa
      601 caaaaaatac tgccatgcag acggaaccga aatgaccatt tcccgtgtct gctgggacac
      661 cggggggatc gatggtgaaa ttgtctatca gaggtcaaaa aaacacggtg ttttccgggt
      721 gctgccggta aaaggcgcat ctgtctatgg caagccggtg atcaccatgc cgaaaacccg
      781 caatcagcgg ggcgtgtatc tgtgtgaagt ggggacggac accgcaaaag aaattctcta
      841 tgcccgtatg aaagccgatc ccactcctgc ggatgaagcc acgtcgtatg ccatccgttt
      901 tcctgatgat ccggagattt tttcgcagac agaggcgcag caactggtcg cggaagagct
//
LOCUS       DAZFXT010000359          960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.362, whole genome shotgun sequence.
ACCESSION   DAZFXT010000359 DAZFXT010000000
VERSION     DAZFXT010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.362"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..299
                     /locus_tag="ACHKW0_005275"
     CDS             <1..299
                     /locus_tag="ACHKW0_005275"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7477893.1"
                     /translation="SKAGERYRRAVALIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDL
                     DNILKAPLDALTHAGLLMDDEQFDEINIVRGKPVSGGRLGVKIYPIMLEGQVKK"
     gene            296..>960
                     /locus_tag="ACHKW0_005276"
     CDS             296..>960
                     /locus_tag="ACHKW0_005276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7477894.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCSCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGC
                     ERLP"
BASE COUNT          258 a          196 c          291 g          215 t
ORIGIN      
        1 tatcaaaagc cggggagcgt tatcgccggg cagtggcgct tattgttcgc cagcagcgac
       61 tgaaattaag cctgtccgga cggctggcaa taaaaattat tgcagagcca ccggataagc
      121 gccgccgtga cctggacaat attctgaaag caccgctgga tgcgctgacg cacgcggggt
      181 tgttaatgga cgatgagcag tttgatgaaa tcaatattgt acgtggaaag ccagtatctg
      241 gtggacgtct gggggtgaag atttacccca taatgcttga agggcaggtc aaaaaatgaa
      301 actggaagat ttaccgaaat actactcccc aaaatcccct ggcctgaccg atgcatcggc
      361 ctcaacgtca aaagatgcgc tgagtatcac tgatgtgatg gccgcgcagg gcatgacaca
      421 gaatcgggct gagatggggt tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
      481 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
      541 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
      601 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgctcct gctgtcacgg
      661 aaaaaaattt attgaaagcg aggtttttac aaacaagatc cagtatccgg atggtaagcc
      721 gccggtatgg gcaaagtgta cgaaaggtgt gtatccgtct tactgggaag aatggaaaaa
      781 agtcagggag gtggtaaaag ttgcctgtcc ggagtgtggc ggaaagggtg aggtttccac
      841 cgcctgtaag gattgccgtg ggcgtggtgt cgccattcat cgtgaagagt cggtaaaacg
      901 tggtatgcct gttatcagag actgccagcg ttgtggtggt cgtggctgtg aaagactacc
//
LOCUS       DAZFXT010000360          960 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.363, whole genome shotgun sequence.
ACCESSION   DAZFXT010000360 DAZFXT010000000
VERSION     DAZFXT010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.363"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..299
                     /locus_tag="ACHKW0_005277"
     CDS             <1..299
                     /locus_tag="ACHKW0_005277"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HFO7477895.1"
                     /translation="SEEGKRYRRAVALIVRQQRLKLSLSGRLAIKIIAEPPDKRRRDL
                     DNILKAPLDALTHAGLLMDDEQFDEINIVRAQPVSGGRLGVKIYPIMLEGQVKK"
     gene            296..>960
                     /locus_tag="ACHKW0_005278"
     CDS             296..>960
                     /locus_tag="ACHKW0_005278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HFO7477896.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDTLSITDVMAAQGMTQNW
                     AEMGFSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNKIQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLP"
BASE COUNT          251 a          194 c          296 g          219 t
ORIGIN      
        1 tctcggagga gggaaagcgt tatcgccggg ctgtggcgct tattgttcgc cagcagcggc
       61 tgaaattaag cctgtccgga aggctggcga taaagattat tgccgagcca ccggataagc
      121 gccgccgtga cttggacaat attctgaaag cgccgctgga tgcgctgacg catgcggggt
      181 tgctaatgga cgatgagcag tttgatgaaa tcaatatcgt tcgtgctcag ccagtatctg
      241 gtggacgtct gggggtgaag atttacccca taatgcttga agggcaggtc aaaaaatgaa
      301 actggaagat ttaccgaaat actactcccc aaaatccccc ggcctgactg atgcatcggc
      361 ctcaacgtcg aaagatacgc tgagtatcac tgatgtgatg gctgcgcagg gcatgacaca
      421 gaattgggct gagatggggt tttctgcgtt ccttgggaaa atgggcatta gtatgaatga
      481 cagagagcgg gcaacagaat tgctgacaga atatgcactc agtcggtgtg atcgcgtggc
      541 ggcgttaaga aaactcccgg cagaaataaa accggcagtg atgcgtatta tggcttcgta
      601 tgcgtttgaa gattatgccc gtagcgcggc gagcaaaaaa cagtgcccct gttgtcacgg
      661 aaaaaaattt attgaaagcg aggtttttac aaacaagatc cagtatccgg atggtaagcc
      721 gccagtgtgg gcaaagtgca caaaaggcgt gtatccgtct tactgggagg aatggaaaaa
      781 agtcagggag gtggtaaaag ttgcctgtcc ggagtgtgga gggaaggggg aggtttccac
      841 cgcctgtaaa gattgtcgtg ggcgcggtgt tgccattcat cgtgaagagt cggtaaaacg
      901 tggtatgcct gttatcagag actgccagcg ttgtggtggt cgtggctatg aaagattacc
//
LOCUS       DAZFXT010000361          955 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.364, whole genome shotgun sequence.
ACCESSION   DAZFXT010000361 DAZFXT010000000
VERSION     DAZFXT010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.364"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..254
                     /locus_tag="ACHKW0_005279"
     CDS             <1..254
                     /locus_tag="ACHKW0_005279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HFO7477897.1"
                     /translation="VPLTEPQKAGIASFCPYNIGPGKCFPSTFYKRINAGDRKGACEA
                     IRWWIKDGGRDCRIRSNNCYGQVSRRDQESALACWDIDR"
     gene            475..588
                     /locus_tag="ACHKW0_005280"
     CDS             475..588
                     /locus_tag="ACHKW0_005280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477898.1"
                     /translation="MKGIEVETPASLDLTRAAAFAIRIVAIAVLVWAIRWW"
     gene            590..895
                     /locus_tag="ACHKW0_005281"
                     /pseudo
     CDS             590..895
                     /locus_tag="ACHKW0_005281"
                     /inference="COORDINATES: protein motif:HMM:NF015218.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
     gene            843..>955
                     /locus_tag="ACHKW0_005282"
     CDS             843..>955
                     /locus_tag="ACHKW0_005282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309243.1"
                     /note="In lambda-like phage, genes encoding the i-spanin
                     subunit Rz, and the o-spanin subunit Rz1 (this protein)
                     overlap; Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA];
                     GO_process: GO:0044659 - viral release from host cell by
                     cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysis system o-spanin lipoprotein Rz1"
                     /protein_id="HFO7477899.1"
                     /translation="MLPLVVSACGSTPPAPVPCVKPPAPPAWIMQPAPDWQT"
BASE COUNT          232 a          215 c          284 g          224 t
ORIGIN      
        1 gggtgccgct gaccgaaccc cagaaagcgg ggatcgcgtc attctgtccg tacaacattg
       61 gccccggtaa gtgcttcccg tcgacgtttt ataaacgaat taatgcaggc gatcgaaaag
      121 gtgcctgtga ggcgattcgc tggtggatta aggacggtgg tagagactgc cgtatccgtt
      181 caaataattg ctacggtcag gtctcacggc gtgaccagga gagcgcgctg gcgtgctggg
      241 acatcgacag atagcagaat attttcctga aaaatgacgt tggccaacgc ggggggataa
      301 cacgaaatcc tgaaaactgg taaaacctaa gtgaataaaa gtaaaaaccc cgtttgttgg
      361 cagcaagcgg ggttttgtgt tttctgacct tgagtaaggc aagggagaaa ttatgggtag
      421 ggaggtactt tccctgtgag gaagtataaa agattctttc tgaggttgtc cattatgaaa
      481 ggcattgaag tggagacgcc agccagtctg gatttaacaa gagcggcagc ttttgccatt
      541 cgtattgtgg ccattgctgt tctggtctgg gcaatccgtt ggtggtgata tgaaccgtgt
      601 tctgtgcgtg gttatcattg tcctgctggt ggcctgtggt gcgcttagtc tggggctgaa
      661 tcattaccgt gatcacgcca tcatctacaa agagcagcgc gataaaaaag ccagtgagct
      721 ggagctggcg aacgcgacaa ttactgatat gcagatacgc cagcgtgatg tcgctgcact
      781 tgatgccaga tactcgaggg aattagccga tgcgagagct gaaaatgaaa ctctgcgtgc
      841 tgatgttgcc gctggtcgta agcgcctgcg gatcaacgcc acctgctccg gtaccgtgcg
      901 tgaagccacc ggcacctccg gcgtggataa tgcaaccggc ccccgactgg cagac
//
LOCUS       DAZFXT010000362          947 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.365, whole genome shotgun sequence.
ACCESSION   DAZFXT010000362 DAZFXT010000000
VERSION     DAZFXT010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.365"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>947
                     /locus_tag="ACHKW0_005283"
     CDS             <1..>947
                     /locus_tag="ACHKW0_005283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease"
                     /protein_id="HFO7477900.1"
                     /translation="NEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVER
                     EGPFYFLFTDKDGEKYGRANKLSGLDKALSAGATEITKEEYFARKNGTYSGSQQNTGA
                     SDTTAQPGSVKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWVKGI
                     QTRDSVNQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGG
                     NCPDCGAVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETG
                     EASDPLIKANGHHNLTSTSRAGIHLMIDLETMGKNPDAP"
BASE COUNT          312 a          227 c          243 g          165 t
ORIGIN      
        1 aatgaagaaa cagacctggg cactgttgat gttctccagt catcagaaac acaaactgat
       61 gcagtggaaa ctgaggtatc tgatatccca aaaaatgaaa ccgcgccgga agctgaacca
      121 tctgtagagc gtgaggggcc gttctacttc ctcttcaccg acaaggatgg cgaaaaatac
      181 ggtcgcgcaa acaaactttc tggtctggat aaggcgctgt ctgctggggc tactgaaatc
      241 acgaaagaag aatatttcgc ccgcaaaaac ggtacatact caggttcaca acaaaatact
      301 ggtgcatctg acacgaccgc acaaccaggg tcagtaaaag ttaccgctga cgaagtaaac
      361 aaaattatgc aggcagccaa tatcagccag cctgacgccg atgaactgct tgcagtatca
      421 cgtggtgaat ttgttgaagg gattagcgac ccgaatgatc cgaaatgggt taaggggatc
      481 cagacccgcg attctgtgaa ccagaaccag caagaaacgg aacagaacga ccagaaagcg
      541 gaacaaaaca gcccaaatac gcaacaaaac gagccagaaa cgaaacaacc tgaaccagta
      601 gtgcaacagg aaccggaaaa gatctgcacc gcctgcggtc agagcggtgg cggcaactgc
      661 cctgattgtg gtgcggtgat gggtgacgca acataccagg aaatattcga tggagagaat
      721 cagcctgaag ttcaggaaaa tgatccggag gaaatggaag gcactgcgca tcagcacaag
      781 gagaacactg gcggcaatca gcatcatgcc agcgatagtg aaactggcga ggcgtcagat
      841 cccttaatta aggcgaacgg tcatcataat ctcacatcca ccagcagagc ggggattcat
      901 ctgatgatcg accttgaaac catgggaaaa aatcccgatg ccccgat
//
LOCUS       DAZFXT010000363          927 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.366, whole genome shotgun sequence.
ACCESSION   DAZFXT010000363 DAZFXT010000000
VERSION     DAZFXT010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.366"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            15..>927
                     /locus_tag="ACHKW0_005284"
     CDS             15..>927
                     /locus_tag="ACHKW0_005284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477901.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLLETTSKGDRNPSSVRLLIQLQRNGNWVTEKDVTINGKTT
                     SQFLASVILDNLPPRPFNIRMVRETADSTSDQLQNKTLWSSYTEIIDVKQCYPNTAIV
                     GLQVDAEQFGGQQMTVNYHIRGRIIQVPSNYDPEKRTYSGIWDGSLKPAYSNNPAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYAI"
BASE COUNT          231 a          248 c          288 g          160 t
ORIGIN      
        1 taaagggtga gacaatgggc aaaggtggcg gcaaggcgca cacgccggta gaggcaaagg
       61 acaatcttaa gtccacgcag atgatgagcg tgattgatgc gattggtgag ggaccgatag
      121 aaggtccggt gaagggactg cagagtattc tggtgaacaa aaccccacta acggacacgg
      181 acggcaatcc cgtgatacac ggtgtgaccg cggtctggcg tgccggggag caggagcaga
      241 caccaccgga aggctttgag tcctccggag ctgaaaccgc actgggcgtg gaagtgacga
      301 aagcaaagcc ggtgacgcgc accattacgt ccgcgaacat tgaccgcctg cgggtcacct
      361 tcggggtgca gtcactgttg gagaccacct caaagggcga ccgtaatccc tcttctgtcc
      421 gcctgctgat tcagttacag cgtaacggta actgggtgac agaaaaggac gtcaccatta
      481 acggcaagac cacctcacag ttcctggcct cggtgattct ggataatctg cctccccggc
      541 cctttaacat ccggatggtc agggagacag cggacagcac ctcggaccag ctgcagaata
      601 agacgctctg gtcgtcatac accgaaatca tcgatgtgaa acagtgctac ccgaacacgg
      661 cgattgtggg gctgcaggtg gatgcggagc agtttggcgg tcagcagatg acggtgaact
      721 accatatccg aggtcgcatc atccaggtac cgtcaaacta tgacccggaa aaacgcactt
      781 acagcggcat ctgggacggc agcctgaaac cggcatacag caacaacccg gcctggtgcc
      841 tgtgggacat gctgactcac ccgcgctacg gcatgggaaa acgtctgggg gcggcggatg
      901 tggacaagtg ggcgctgtat gccatcg
//
LOCUS       DAZFXT010000364          923 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.367, whole genome shotgun sequence.
ACCESSION   DAZFXT010000364 DAZFXT010000000
VERSION     DAZFXT010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.367"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(310..582)
                     /locus_tag="ACHKW0_005285"
     CDS             complement(310..582)
                     /locus_tag="ACHKW0_005285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477902.1"
                     /translation="MTENVKSELLLLMADNNEATSSILADPYGKISHKTLDIITTTLT
                     PLMLQRLKHNINAWVNEELSPPCLWDSRYACQQKMRIFNLLSPKLR"
     gene            742..>923
                     /locus_tag="ACHKW0_005286"
     CDS             742..>923
                     /locus_tag="ACHKW0_005286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7477903.1"
                     /translation="MNEKIKYGLSAAVLALIGAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGAILVDG"
BASE COUNT          257 a          166 c          227 g          273 t
ORIGIN      
        1 aacctgtatt tcaaaatcag agaggaccgt cgtaaggtgg cgcggggaga gtagtcgatg
       61 aataaacaat acgaactggt tgtaaaatga atatttctaa ctgaaaaaac gttccatgag
      121 gtaagaaaag gtcacaggca atcaataaca ggacgtgatg aaagaccctt gcatttgtgc
      181 gctttctctt tagatagcag cagatactga aaatctgagt tgtcggggag tcagggatac
      241 agctgtgcaa gagttggtca ttgtgattcc attgaaatcc tgtatgccat gaagggcagg
      301 attttatggc tacctgagct ttggtgatag taagttgaaa attcgcattt tttgctgaca
      361 tgcgtaacga gaatcccata agcagggagg acttaattct tcattaaccc atgcgttgat
      421 attatgtttc agccgttgaa gcatcagcgg tgttaatgtt gtggtaataa tatccagcgt
      481 tttatgtgag atcttaccgt aagggtctgc aagaatgctg cttgttgctt cgttattatc
      541 tgccatcaga agaagtaact ctgatttaac gttttctgtc attagttgta aaaatcttct
      601 gcgcaaactt tctttactgt tcatttatat ggcttcattt gttgtaatct gctgcgtctc
      661 aagggatatg tttatgagag cgaccatgag tgttggatta tatacctaac atatcaaggg
      721 attagaaatc gataaatccc catgaacgaa aaaataaaat acggcctgtc ggctgccgtt
      781 ctggcgctga ttggtgcagg tgcttctgcg cctgaaatcc tcgaccagtt tctggatgaa
      841 aaggaaggta accacaccac ggcataccgt gatggtgcgg ggatctggac catctgccgt
      901 ggtgccattc tggtggatgg taa
//
LOCUS       DAZFXT010000365          915 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.368, whole genome shotgun sequence.
ACCESSION   DAZFXT010000365 DAZFXT010000000
VERSION     DAZFXT010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.368"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..607)
                     /gene="fimE"
                     /locus_tag="ACHKW0_005287"
     CDS             complement(11..607)
                     /gene="fimE"
                     /locus_tag="ACHKW0_005287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HFO7477904.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEV"
BASE COUNT          291 a          198 c          175 g          251 t
ORIGIN      
        1 atgagttaaa tcaaacctct tctcttttta atttttcgtt tatgagatta tttctttccc
       61 atagtccggc aaaacgagca gcattactgg cggtataacg tacagtatgg cgaatatttc
      121 gatgcccgag ataatcctga attaaacggg tatcggcacc acgctccgcc agttcataac
      181 cacaagcatg ccttaacata tgaggatgag tctgcgttac ggttccagct tcaataccgg
      241 catcgcgaat aatgcgatag gcctgctggc gagaaagccg actcccgcgg cgagaaataa
      301 atatagcgtc ggtccggtca gcgcctttcc agttagcacg ttcctgggtc cagcgttcca
      361 cggcttcacg ctcatcaaaa cgtaacgggt gaacggtaga aaatccgttc ttcagtcggc
      421 gaatatttat tcgaccttca ttaaggtcaa gatcctgata atgcagatca agcagttcac
      481 taatacgcat cccatgccga tatgccaaca gaataagaca ataatctctg gctcccgttg
      541 ccccgtaaca aaccgcctgc atcatggcct gaacttcttt accggtaaga taacgacgtt
      601 tactcacaat agtagtactc ctaactgaga tatatttgaa tgaataccta taggaaacct
      661 caatcggtca aaattagccc aatagataaa aagataacca cacacggagt gatgtggcta
      721 tcagtcaatt acattaaata tcatacaaaa ataaaatatc actgatgtga caacatctat
      781 tttcattgat ttgattcaat ataacaatag attgcagtgg attaacttcc agttgcttaa
      841 aaaataaaga aaaaagtgcg gaaacaggag aatttaagtg atgtgtaaat ttatgaatga
      901 ttttgtttat ttatt
//
LOCUS       DAZFXT010000366          914 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.369, whole genome shotgun sequence.
ACCESSION   DAZFXT010000366 DAZFXT010000000
VERSION     DAZFXT010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..914
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.369"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>914
                     /locus_tag="ACHKW0_005288"
     CDS             <1..>914
                     /locus_tag="ACHKW0_005288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO7477905.1"
                     /translation="TPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEICYR
                     YDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAGLKRVVKKEHADG
                     SVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLTTPDGRASAFYYNHHNQLTSATGP
                     DGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATEDATGSRKTMTWSRYGQ
                     LLTFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYHAYDSRGQLTAVKDTQGHETRYEY
                     NIAGDLTAVIAPDGSRNGTQYDAWGKAI"
BASE COUNT          228 a          256 c          284 g          146 t
ORIGIN      
        1 ggacgccgcg cggcgaactg gcggtggtgt atgaccgtag tggcaaacag gtgcgcagct
       61 ttacttacga tgataaatac cggggccgga tggtggcgca ccgtcacacg ggccggccgg
      121 aaatctgtta ccgttacgac agcgacgggc gggtgacaga acagctaaac ccggcaggct
      181 taagttacac gtatcagtat gaaaaagacc gcatcaccat caccgacagc ctgaaccgcc
      241 gtgaagtgct gcacacgcag ggcgaagccg ggctgaagcg ggtggtgaaa aaggaacacg
      301 cggacggcag cgtcacgcag agtcagtttg acgcggtggg caggcttaag gcacagacgg
      361 atgccgcagg ccggacaaca gaatacagcc cggatgtggt gacgggcctc accacgccgg
      421 atggcagggc atcggcgttt tactataacc accacaacca gttaacgtca gccaccgggc
      481 ctgacgggct ggaattgcgc cgggaatatg atgaatccgg ccgtctgatt caggaaactg
      541 cccctgacgg cgatatcacc cgctaccgtt atgataatcc acacagtgac ttaccctgcg
      601 caacggaaga tgccaccggc agccggaaaa ccatgacgtg gagccgttac ggtcagttgc
      661 tgaccttcac tgactgttcc ggttatgtaa cccgctatga ccatgaccgt tttggtcagg
      721 tgacggcggt gcaccgcgag gaaggcctga gccagtacca cgcatacgac agccgtggac
      781 agttaactgc ggtgaaagac acgcagggcc atgaaacgcg gtatgaatac aacatcgccg
      841 gtgacctgac cgccgtcatt gccccggacg gcagcagaaa cgggacacag tacgatgcgt
      901 ggggaaaggc catc
//
LOCUS       DAZFXT010000367          911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.370, whole genome shotgun sequence.
ACCESSION   DAZFXT010000367 DAZFXT010000000
VERSION     DAZFXT010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.370"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHKW0_005289"
     CDS             <1..75
                     /locus_tag="ACHKW0_005289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477906.1"
                     /translation="EGIRITDIDTSGIFDSDDMAIKAA"
     gene            148..288
                     /locus_tag="ACHKW0_005290"
     CDS             148..288
                     /locus_tag="ACHKW0_005290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO7477907.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NG"
     gene            281..424
                     /gene="kil"
                     /locus_tag="ACHKW0_005291"
     CDS             281..424
                     /gene="kil"
                     /locus_tag="ACHKW0_005291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO7477908.1"
                     /translation="MDKTLMAIQTKFTIATFIGDEKMFREAVDAYKKWILIQKLRSSK
                     SIH"
     gene            500..796
                     /locus_tag="ACHKW0_005292"
     CDS             500..796
                     /locus_tag="ACHKW0_005292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7477909.1"
                     /translation="MNAYYIQDRLEAQSWARHYQLIAREEKEAELADDMEKGLPQHLF
                     ESLCIDHLQRCGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            802..>911
                     /locus_tag="ACHKW0_005293"
     CDS             802..>911
                     /locus_tag="ACHKW0_005293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7477910.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKG"
BASE COUNT          263 a          213 c          210 g          225 t
ORIGIN      
        1 gaaggaataa gaattaccga tatcgataca tcaggaatat ttgattcaga tgatatggct
       61 atcaaggccg cctgagtgcg gctttaccgc ataccaataa cgcttcactc gaggcgtttt
      121 tcgttatgta taaataagga gcacaccatg caatatgcca ttgcagggtg gcctgttgct
      181 ggctgccctt ccgaatcttt acttgagcga atcacccgta aattacgtga cggatggaaa
      241 cgccttatcg acatacttaa tcagccagga gttccaaaaa atggataaaa cacttatggc
      301 tatccagact aaattcacta tcgccacttt tattggcgat gaaaagatgt ttcgtgaggc
      361 cgtcgacgct tataaaaaat ggatattaat acagaaactg agatcaagca aaagcattca
      421 ctaaccccct ttcctgtttt cctaatcagc ctggcatttc gcgggcgata ttttcacagc
      481 cattttcagg agttcagcca tgaacgctta ttacattcag gatcgtcttg aggctcagag
      541 ctgggcacgt cactaccagc tgatcgcccg tgaagagaaa gaggcagaac tggcagacga
      601 catggaaaaa ggtcttccac aacatctgtt tgaatcactc tgcatcgatc atttgcaacg
      661 ctgcggggcc agcaaaaaag ccattacccg tgcgtttgat gacgatgttg agtttcagga
      721 gcgcatggca gaacacatcc ggtacatggt tgaaaccatt gctcaccacc aggttgatat
      781 tgattcagag gtataaaacg gatgagtaca gcactcgcaa cgctggcagg gaagctggct
      841 gaacgtgtcg gcatggattc tgtcgaccca caggaactga tcaccactct tcgccagacg
      901 gcatttaaag g
//
LOCUS       DAZFXT010000368          911 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.371, whole genome shotgun sequence.
ACCESSION   DAZFXT010000368 DAZFXT010000000
VERSION     DAZFXT010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.371"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..284
                     /locus_tag="ACHKW0_005294"
     CDS             <1..284
                     /locus_tag="ACHKW0_005294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477911.1"
                     /translation="LTDSHKCKGTFVDFVNLNGFQIDPQFEPTPKAAESALTIEVADN
                     DNNFKLKIMRGAIGDEKSNKPVILTDNKCEIVIKLSQANYDELKRYRDS"
     gene            287..>911
                     /locus_tag="ACHKW0_005295"
     CDS             287..>911
                     /locus_tag="ACHKW0_005295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000150292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477912.1"
                     /translation="MTIADDLSRLAQIINGASSRVEGYYTVISLEESIVIVNSSEIIR
                     LLQSIGYKKATTCIENNEIWLDRQASSWDDAIIYENVESFWSRVNTQNALPKNYIIGT
                     PLILPTSKNESIEKIHIFFMWKDILSLIADHHNSDCSVLFFTNEDKSYTVELTHFLQY
                     SEINRLSNSSLKYEIIKELLDTIKINDLHKSERKLVIRSAINEVFKAN"
BASE COUNT          327 a          161 c          162 g          261 t
ORIGIN      
        1 gcctgactga ttcacacaaa tgtaagggaa cgtttgtcga ttttgttaat cttaatggtt
       61 tccagattga ccctcaattt gaaccaactc ctaaagcagc tgaaagcgcg cttacaattg
      121 aagtggcaga taacgacaat aattttaagt tgaaaataat gcgtggagct attggagacg
      181 aaaagtcaaa taagcctgta attctcactg acaacaaatg cgaaattgtg ataaaattga
      241 gtcaggcaaa ttatgatgaa ctcaaaagat atagagacag ctaataatga caattgctga
      301 tgacttatca agattagcgc agattattaa cggagcctca agcagagttg agggctacta
      361 cactgtcata agtcttgaag aaagcattgt tattgtaaat agttctgaaa taattagact
      421 gttacaatct ataggttata aaaaggcaac aacctgcatc gaaaataatg aaatttggct
      481 agatcgccaa gcttcatctt gggatgacgc tataatttat gagaatgttg agtctttttg
      541 gtctagagta aacacccaaa acgctcttcc gaaaaattat atcatcggaa cgccgttaat
      601 actccctact tctaagaatg aaagcatcga aaaaatccat attttcttta tgtggaaaga
      661 tatcctttca ttaattgctg atcatcataa cagtgactgc tctgtcttat ttttcaccaa
      721 tgaagacaaa agttatacag tcgaactcac gcatttttta caatatagcg agattaatcg
      781 cttatcgaac tcgtctctta aatatgaaat tataaaggag cttctcgaca caataaaaat
      841 caatgattta cacaaaagcg agcgcaagct cgttatacgc tcagccataa acgaagtatt
      901 taaagcaaat g
//
LOCUS       DAZFXT010000369          901 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.372, whole genome shotgun sequence.
ACCESSION   DAZFXT010000369 DAZFXT010000000
VERSION     DAZFXT010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.372"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>901
                     /locus_tag="ACHKW0_005296"
     rRNA            <1..>901
                     /locus_tag="ACHKW0_005296"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          246 a          197 c          277 g          181 t
ORIGIN      
        1 atcagaagtg cgaatgctga cataagtaac gataaagcgg gtgaaaagcc cgctcgccgg
       61 aagaccaagg gttcctgtcc aacgttaatc ggggcagggt gagtcgaccc ctaaggcgag
      121 gccgaaaggc gtagtcgatg ggaaacaggt taatattcct gtacttggtg ttactgcgaa
      181 ggggggacgg agaaggctat gttggccggg cgacggttgt cccggtttaa gcgtgtaggc
      241 tggttttcca ggcaaatccg gaaaatcaag gctgaggcgt gatgacgagg cactacggtg
      301 ctgaagcaac aaatgccctg cttccaggaa aagcctctaa gcatcaggta acatcaaatc
      361 gtaccccaaa ccgacacagg tggtcaggta gagaatacca aggcgcttga gagaactcgg
      421 gtgaaggaac taggcaaaat ggtgccgtaa cttcgggaga aggcacgctg atatgtaggt
      481 gaagcgactt gctcgtggag ctgaaatcag tcgaagatac cagctggctg caactgttta
      541 ttaaaaacac agcactgtgc aaacacgaaa gtggacgtat acggtgtgac gcctgcccgg
      601 tgccggaagg ttaattgatg gggtcagcgc aagcgaagct cttgatcgaa gccccggtaa
      661 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      721 cctgcacgaa tggcgtaatg atggccaggc tgtctccacc cgagactcag tgaaattgaa
      781 ctcgctgtga agatgcagtg tacccgcggc aagacggaaa gaccccgtga acctttacta
      841 tagcttgaca ctgaacattg agccttgatg tgtaggatag gtgggaggct ttgaagtgtg
      901 g
//
LOCUS       DAZFXT010000370          838 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.373, whole genome shotgun sequence.
ACCESSION   DAZFXT010000370 DAZFXT010000000
VERSION     DAZFXT010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.373"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            95..>838
                     /locus_tag="ACHKW0_005297"
     CDS             95..>838
                     /locus_tag="ACHKW0_005297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO7477913.1"
                     /translation="MIISSDNLSVIIPVYNEEKNVINILQSLECQSLTGFNVIVIDDG
                     SKDMTASLVKEYKPSSYSLSLIQQSNMGAARARENAINFTHSEYIAFIDSDDSLSSDA
                     LEKALAPMLDNKDIDISLFELVHIKNLNHDTNNIFVPYSTTKLICGEEAFANCISYWG
                     LHGFGIYKRKLIQKSYGIYYKYNKNKENYINNDEVISRISFGLSKNIYLSSGRYFFVQ
                     NMDSTTRRINESYYKVINNAVYLREYIDEE"
BASE COUNT          307 a          103 c          138 g          290 t
ORIGIN      
        1 cgttagtatt gcctacagca tcacttatgc attgtatttt atagtggtgt gtattattta
       61 ttgttggcat ctgacaaaaa aggccctata acaaatgata atatcttcag ataacctaag
      121 tgttattatc ccagtttata atgaagagaa gaatgttatt aatattcttc aatctcttga
      181 atgccagtct ttaacgggat ttaatgttat tgtcattgat gatggttcaa aagatatgac
      241 tgcttctctt gtaaaagaat ataaaccatc atcatatagt ttaagcttaa tacaacaaag
      301 taatatgggg gctgctcggg caagggagaa tgccatcaat tttactcata gtgagtatat
      361 tgcatttata gatagtgatg actctttaag ttcagatgct cttgaaaaag cattagctcc
      421 gatgctggat aataaagata tcgacatctc actatttgaa ctggtacata ttaaaaactt
      481 aaatcatgat actaataaca tatttgtacc ttattctaca accaaattaa tatgtggaga
      541 agaggctttt gctaattgta tttcatattg gggattgcat gggtttggta tctacaaaag
      601 aaaattgatt caaaaatcct atggtattta ttataaatat aataaaaaca aagaaaatta
      661 tataaataat gatgaggtta tttcaagaat cagttttgga ttatctaaaa atatctattt
      721 atcatctgga agatatttct ttgtacagaa catggattca acaacaagaa gaataaatga
      781 aagttattat aaagttataa ataatgctgt ttatttaaga gagtatatag atgaagaa
//
LOCUS       DAZFXT010000371          837 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.374, whole genome shotgun sequence.
ACCESSION   DAZFXT010000371 DAZFXT010000000
VERSION     DAZFXT010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..837
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.374"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>837
                     /locus_tag="ACHKW0_005298"
     CDS             <1..>837
                     /locus_tag="ACHKW0_005298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail length tape measure family protein"
                     /protein_id="HFO7477914.1"
                     /translation="QFDAINQSVARFASASGVEVDKVAEAFGKLTTDPTSGLMAMARQ
                     FRNVTAEQIAYVAQLQRSGDEAGALQAANDIATKGFDEQTRRLKENMGTLETWADKTG
                     KAFKSMWDAILDIGRPESSADMLASAQKAFDEADKKWQWYQSRSQRRGKTSSFRANLQ
                     GAWNDRENARLGLAAATLQSDMEKAGELAARDRAERDASQLKYTGEAQKGYERLLTPL
                     EKYTARQEELNKALKDGKILQADYNTLMAAAKKDYESTLKKPKSSGVKVSAGERQEDQ
                     AHA"
BASE COUNT          212 a          204 c          281 g          140 t
ORIGIN      
        1 acagtttgat gccatcaacc agagtgtcgc gcgttttgct tctgcatccg gtgtggaggt
       61 ggacaaggtt gcagaggctt tcggaaaact gaccaccgac ccgacgtcgg ggctgatggc
      121 gatggcgcgc cagttccgta acgtgacggc agagcagatt gcgtatgttg cgcagctgca
      181 gcgttccggt gatgaggccg gggccttaca ggcggcgaac gatatcgcca cgaaaggctt
      241 tgatgagcag acccgtcgcc tgaaagaaaa catggggaca ctggagacct gggcggataa
      301 aacagggaag gcattcaaat cgatgtggga tgccattctg gatatcggtc gtcctgagtc
      361 ctcagcggat atgctcgcca gtgcgcagaa ggcatttgat gaggcggata aaaaatggca
      421 gtggtaccag agccggagcc agcgccgcgg taaaacctct tctttccggg ccaaccttca
      481 gggcgcatgg aatgaccggg aaaatgcccg gctggggctg gcagcggcca cgctgcagtc
      541 ggatatggaa aaagccggtg aactggccgc cagggaccgg gccgaacggg acgcatcaca
      601 gctgaagtat accggagagg cgcagaaggg gtatgagcgt ctgctgacgc cgctggagaa
      661 atataccgcc cgtcaggaag aactgaataa ggccctgaaa gacgggaaaa tcctgcaggc
      721 ggattacaac acgctgatgg cggcggcgaa aaaggattat gaatcgacgc tgaaaaagcc
      781 gaagtcgtca ggagtcaaag tgtcagccgg tgagcgtcag gaagaccagg cgcatgc
//
LOCUS       DAZFXT010000372          835 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.375, whole genome shotgun sequence.
ACCESSION   DAZFXT010000372 DAZFXT010000000
VERSION     DAZFXT010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.375"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..296)
                     /locus_tag="ACHKW0_005299"
     CDS             complement(<1..296)
                     /locus_tag="ACHKW0_005299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477915.1"
                     /translation="MKTFRWKVKPDMEVNSQPSVREVRFGDGYSQRMAAGLNADLKTY
                     RVTLSVTREEARHLEAFLAEHGGWKAFLWTPPYAWRQIKVTCAAWSSRVRMLRV"
     gene            complement(293..>835)
                     /locus_tag="ACHKW0_005300"
     CDS             complement(293..>835)
                     /locus_tag="ACHKW0_005300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7477916.1"
                     /translation="EQSWRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAMGGAFGGGAS
                     ASTGTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLM
                     RGYAEGGYVGGAGSPAQMRRAEGISFNQNNHVVIQNDGTNGQAGPQLMKAVYDMARKG
                     AQDEIQAQMRDGGVFSGGRR"
BASE COUNT          174 a          305 c          195 g          161 t
ORIGIN      
        1 tcaacccgca gcatgcgaac ccgtgatgac caggcggcac aggtcacctt tatctgccgc
       61 caggcataag gcggtgtcca cagaaacgcc ttccagccac cgtgctctgc caggaatgcc
      121 tccagatgtc gggcctcctc ccgggtcacg gaaagcgtca cacggtatgt tttcaggtca
      181 gcattcagcc ccgccgccat acgctgcgaa tacccgtcac caaaacgcac ttcacgcacc
      241 gatggctgcg agttcacctc catatccggc ttcactttcc agcgaaatgt tttcatcgcc
      301 tgcctccgga aaagacgccg ccatcacgca tctgcgcctg aatctcatcc tgcgccccct
      361 tgcgggccat gtcatacacc gccttcatca gctgcggccc cgcctgtccg ttggtgccgt
      421 cgttctgaat caccacgtga ttgttctgat taaaactaat gccttccgcc cgccgcatct
      481 gcgccggact tccggcaccg cccacataac caccttccgc atacccgcgc atcagacgat
      541 acaggttgcc gacgccaatc cggctggttg cctccttcgt gaagacaaac tccccgcggt
      601 ggacaatacc ggcaggttcg tatttgccac cggttcccgt aaatcctccg gtcgcgaaat
      661 ggaagttcgc cgccgcagcc tgaatggccg tccccgtgga ggcagacgcc ccaccaccga
      721 aagcaccacc catggcgctg ccaatactcc cgacaatccc caccatggcc tgcttcagaa
      781 aaatctctgt cagcatggag agcacagaac gggtgaaacc acgccagctc tgttc
//
LOCUS       DAZFXT010000373          827 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.376, whole genome shotgun sequence.
ACCESSION   DAZFXT010000373 DAZFXT010000000
VERSION     DAZFXT010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..827
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.376"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            3..>827
                     /gene="nleB2"
                     /locus_tag="ACHKW0_005301"
     CDS             3..>827
                     /gene="nleB2"
                     /locus_tag="ACHKW0_005301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector arginine
                     glycosyltransferase NleB2"
                     /protein_id="HFO7477917.1"
                     /translation="MVQSSPCQTVSFAGKEYELKVIDEKTPILFQWFEPNPERYKKDE
                     VPIVNTKQHPYLDNVTNAARIESDRMIGIFVDGDFSVNQKTAFSKLERDFENVMIIYR
                     EDVDFSMYDRKLSDIYHDIICEQRLRTEDKRDEYLLNLLEKELREISKAQDSLISMYA
                     KKRNHAWFDFFRNLALLKAGEIFRCTYNTKNHGISFGEGCIYLDMDMILTGKLGTIYA
                     PDGISMHVDRRNDSVNIENSAIIVNRSNHPALLEGLSFMHSKVDAHPYYDGLGKGVK"
BASE COUNT          278 a          108 c          181 g          260 t
ORIGIN      
        1 atatagtgca gagttcaccc tgtcaaacgg tttcttttgc cggaaaggaa tatgagttaa
       61 aggtcattga tgaaaaaacg cctattcttt ttcagtggtt tgaacctaat cctgaacgat
      121 ataagaaaga tgaggttcca atagttaata ctaagcagca tccctattta gataatgtca
      181 caaatgcggc aaggatagag agtgatcgta tgataggtat ttttgttgat ggcgattttt
      241 cagtcaacca aaagactgct ttttcaaaat tggaacgaga ttttgaaaat gtaatgataa
      301 tctatcggga agatgttgac ttcagtatgt atgacagaaa actatcagat atttatcatg
      361 atattatatg tgaacaaagg ttacgaactg aagacaaaag agatgaatac ttgttgaatc
      421 tgttagagaa agagctgagg gaaatttcaa aggcgcagga ttctttgatt tctatgtatg
      481 caaagaaaag aaatcatgca tggtttgatt tcttcagaaa tttagcctta ttaaaagcag
      541 gagagatatt caggtgcaca tataatacaa agaatcacgg tatttcattc ggggaggggt
      601 gtatctatct tgatatggat atgatactta caggtaagct tggtacaata tatgctcctg
      661 atggaatttc aatgcatgtg gatcgtcgta atgatagtgt aaatattgaa aatagtgcaa
      721 taattgttaa ccgtagtaat catcctgctc tacttgaggg actttctttt atgcatagta
      781 aagtagatgc tcatccatat tatgatggtt tggggaaagg agttaag
//
LOCUS       DAZFXT010000374          826 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.377, whole genome shotgun sequence.
ACCESSION   DAZFXT010000374 DAZFXT010000000
VERSION     DAZFXT010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..826
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.377"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..298
                     /locus_tag="ACHKW0_005302"
     CDS             <1..298
                     /locus_tag="ACHKW0_005302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HFO7477918.1"
                     /translation="GELEITAVNEVYLRNNNLTVELLGRGFAWLDTGTHDSLIEAGSF
                     VETVQKRQGMMVACPEEIAWRNGWLNHDELQAKGEFLKKNHYGQYLLRLIKSHE"
     gene            291..698
                     /locus_tag="ACHKW0_005303"
     CDS             291..698
                     /locus_tag="ACHKW0_005303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FdtA/QdtA family cupin domain-containing
                     protein"
                     /protein_id="HFO7477919.1"
                     /translation="MNNNVKLIHFDKKGDNRGFLVVVEQNKDIPIEIKRVYYMFDTGS
                     DVRRGYHAHKKLTQIAIPVKGSCKFLLDDGKSVEHVTLDSPSVGLIIEPMIWHEMYDY
                     SSDCVLMVLADDFYDESDYIRNYADFIREITNA"
BASE COUNT          280 a          111 c          178 g          257 t
ORIGIN      
        1 tggtgaatta gaaattacgg cagttaatga agtttatctc agaaataata atttaactgt
       61 tgaattacta ggacgtggtt ttgcatggct ggatacagga acccatgaca gcttgattga
      121 agctgggagt tttgtagaga cagttcaaaa aaggcaaggg atgatggttg cttgtccgga
      181 ggagattgca tggcgaaatg gctggttaaa ccatgatgaa ttacaggcaa aaggtgaatt
      241 tttgaaaaaa aatcactatg gacagtattt actaaggtta attaaatcgc atgaataata
      301 acgtaaaact tatacacttt gataagaaag gtgacaaccg aggctttctt gttgttgttg
      361 agcaaaataa agatatccca attgaaatta aacgagttta ctacatgttt gatactggtt
      421 ctgatgtaag acggggatat cacgcacata aaaaacttac acaaatagca atccctgtta
      481 agggatcttg caagtttttg cttgatgacg gtaaatcggt ggaacatgtt acattagatt
      541 caccgtcagt gggattaatt atagaaccaa tgatttggca tgaaatgtac gattattcat
      601 cagattgtgt tttaatggtg ctggcagatg acttttatga tgaaagtgat tacattcgta
      661 attatgctga tttcatcagg gagataacta atgcgtaatt tgtcgctctt tacaaaaaat
      721 acaaaattaa ttccagttga aataaatgat gcggaattta tttattcact gcggatagat
      781 tctcgtctca ataagcatat ttctacaata aatgggacgg taattg
//
LOCUS       DAZFXT010000375          822 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.378, whole genome shotgun sequence.
ACCESSION   DAZFXT010000375 DAZFXT010000000
VERSION     DAZFXT010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.378"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..133)
                     /locus_tag="ACHKW0_005304"
     CDS             complement(<1..133)
                     /locus_tag="ACHKW0_005304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003979562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraJ"
                     /protein_id="HFO7477920.1"
                     /translation="MYPMDRIQQKHARQIDLLENLTAVIQDYPNPACIRDETGKFIFC
                     "
     gene            complement(320..703)
                     /gene="traM"
                     /locus_tag="ACHKW0_005305"
     CDS             complement(320..703)
                     /gene="traM"
                     /locus_tag="ACHKW0_005305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome DNA-binding protein
                     TraM"
                     /protein_id="HFO7477921.1"
                     /translation="MAKVNMYISNDAYEKINAIIEKRRQEGAREKDVSFSATASMLLE
                     LGLRVHEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFLKNDDE"
BASE COUNT          250 a          180 c          128 g          264 t
ORIGIN      
        1 tgcaaaaaat aaattttcca gtttcgtccc tgatacaggc tggatttgga taatcctgaa
       61 taactgccgt cagattttcc agcagatcta tttgacgagc atgtttttgt tgaatacgat
      121 ccatcggata cataggaacc tcctcacaaa ggattctatg gacagtcgat gcagggagtt
      181 cacgtctccc tgcatcggcg attttcaatt tcaaatttta acgtggcatt aaccacgtaa
      241 ttaatatact aattatcttt cgtactgtta cctttttaaa tcataaaaac tctgatattt
      301 gaacgaagtc aaatttcttt tattcatcat cattttttag aaaaaatcgt tccatctcag
      361 atgatacctt ctccctgata tcttcaacca tattggcata ttcaaacttt ggatttccgg
      421 agacatgagg actgagagac tcaataccca aaattttcgc tactgatgat tgtgttttta
      481 caacgcattc aagaagcaat ttattaaact cagtctgatt aaatgcagac tctttacgct
      541 ccatctgagc ctcatgtaca cgaagcccca gttcaagaag cattgaagct gttgctgaaa
      601 aactgacatc tttttccctt gccccttcct gtcgacgctt ctcaataatc gcatttattt
      661 tttcataggc atcattgctg atatacatgt tcaccttagc cattaaagat tacctttttt
      721 gaacataaaa taaagttaat ttagaaacac taaaattcat aatgcaaaca gggacgcacc
      781 gctagcagtg cccctggcgg tatcctataa agaaacacac cg
//
LOCUS       DAZFXT010000376          786 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.379, whole genome shotgun sequence.
ACCESSION   DAZFXT010000376 DAZFXT010000000
VERSION     DAZFXT010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.379"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..461
                     /locus_tag="ACHKW0_005306"
     CDS             <1..461
                     /locus_tag="ACHKW0_005306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HFO7477922.1"
                     /translation="ILHRGHQQVLFVVTQADKTEPCHEWDMAGIQPSPAQAQNIREKT
                     EAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALPDHAASPLMTRLQDELRTESVRS
                     QAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDSVVSVARAVWNWIFF"
BASE COUNT          171 a          219 c          208 g          188 t
ORIGIN      
        1 acatcctgca ccggggacat cagcaggtgc tgtttgtggt gacgcaggcc gacaaaacgg
       61 agccctgcca tgaatgggat atggccggta ttcagccttc tcccgcacag gcacagaata
      121 ttcgcgaaaa aacggaggcg gtattccgtc tgttccggcc cgtacatccg gttgtggccg
      181 tatcggcccg caccggctgg gaactggata cgctggtcag tgcgctcatg acagcgcttc
      241 ccgaccatgc cgccagtccc ctgatgaccc gcctgcagga cgagctgcgc acggaatctg
      301 tccggtctca ggcccgtgaa cagtttaccg gtgcggtgga ccggatattt gacacggcgg
      361 agagcgtctg tattgcctct gttgcacgca cggttctgcg tgccgtccgt gactcggtgg
      421 tctctgttgc ccgcgctgta tggaactgga ttttcttctg aacactctgc caggggaaat
      481 gcctgattac attgcgttaa ccaactgaaa acatgacccc ccccctcata cccacctctt
      541 tctcttgcgt gacttccctg ctgttaatag aataaaacga tcgataaaac aggtatcgat
      601 cgttactatc gatcgtttat agtggttaat ggtctgcgaa aaggcagctg aatctcttca
      661 tcatgcagaa cggaattgca cacaacggac tgatgcttct ctggcggatg agagagggga
      721 aacatttatg tccgggaaaa accactttat ctgaccatgc tgttttgtac ctgccggtac
      781 ccacat
//
LOCUS       DAZFXT010000377          783 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.380, whole genome shotgun sequence.
ACCESSION   DAZFXT010000377 DAZFXT010000000
VERSION     DAZFXT010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..783
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.380"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(36..>783)
                     /locus_tag="ACHKW0_005307"
     CDS             complement(36..>783)
                     /locus_tag="ACHKW0_005307"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000044123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7477923.1"
                     /translation="SKNEGGHIRMLVYRLTDFLCDATTNVSNEALDVFIKKKAFSKSK
                     SIPVYNGIDTDLFKYDPLQRDKIRHSLNIINDDSLILSVGRLTDAKDYPNLLKALLEL
                     PERYKLVIIGEGEARNEVERTILNYGLEKRVKLLGSIANVATYYSACDIYVSSSKWEG
                     FGLVVAEAMSCQRLVVATNAGGVAEVVGDMNYIVPVSDPQLLANKINEVMEFDHETKM
                     EVMYRNREFVLKMFSIDMIVNQWMKLYSSI"
BASE COUNT          262 a          162 c           98 g          261 t
ORIGIN      
        1 ttatcaaatt ctatattttt ccattaagct atggattaga ttgaagaata caatttcatc
       61 cattgattaa cgatcatatc tattgagaac atttttaaaa caaactctct atttctatac
      121 attacttcca ttttagtttc gtgatcaaac tccataactt cattaatttt atttgccaac
      181 aactgagggt ctgaaacagg tacaatatag ttcatatcac caacaacttc agccacccca
      241 ccggcattag ttgcaacgac caatcgctgg caagacatag cctcagctac aaccaaacca
      301 aagccttccc acttagagga cgatacataa atatcacatg cagaataata agtggcgaca
      361 ttagcaatac tacctagcaa tttcactctt ttttcaaggc cgtaatttaa aatcgtacgc
      421 tcgacttcat ttctggcttc accttcacct attatgacta atttatatcg ttcaggtaat
      481 tctaacaatg cttttaataa gtttggataa tcttttgcat cagtaagtcg gcctacagaa
      541 agtataaggc tatcatcatt aatgatattt aaactatgcc ttattttatc tctttgtaaa
      601 ggatcatatt taaatagatc agtgtcaatg ccattataaa cgggaattga cttcgatttg
      661 ctaaaggctt ttttcttaat aaaaacatca agagcttcat tacttacatt agtcgtagca
      721 tcacataaaa aatcagttaa tctataaacg agcatcctaa tatgcccacc ttcattttta
      781 cta
//
LOCUS       DAZFXT010000378          782 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.381, whole genome shotgun sequence.
ACCESSION   DAZFXT010000378 DAZFXT010000000
VERSION     DAZFXT010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.381"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            166..732
                     /gene="slp"
                     /locus_tag="ACHKW0_005308"
     CDS             166..732
                     /gene="slp"
                     /locus_tag="ACHKW0_005308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HFO7477924.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          239 a          167 c          169 g          207 t
ORIGIN      
        1 atggatactc ataccacgcc ccaaaataac ggaattgaac taaacagaag ggatataaac
       61 atcagacagg tttacgttac tatcaggcat atcacctcag aatcagatga aaactataaa
      121 gaaatatcta ttatggtttt aatatttgtt gataaggata gtaacatgaa catgacaaaa
      181 ggtgcactca tcctcagcct ttcatttttg cttgccgcat gtagttcaat tccgcaaaat
      241 atcaaaggca ataaccaacc tgatattcaa aaaagttttg ttgctgttca taaccagccg
      301 gggttatatg ttggtcaaca agcgcgcttt ggtgggaagg ttatcaacgt tatcaatggc
      361 aaaacggata cgttgttaga aatcgctgta ttaccgttgg atagctatgc gaagcctgat
      421 attgaagcca actatcaggg ccgactgctc gccagacaaa gcggcttcct tgatccagtg
      481 aactatcgta atcactttgt taccatcctc ggtaccattc agggtgaaca accaggcttt
      541 atcaataaag tcccgtataa cttcctggaa gtgaatatgc agggcatcca ggtgtggcat
      601 ttgagagaag tggttaatac cacctataac ctgtgggatt acggctatgg tgcattctgg
      661 ccggaaccgg gctggggtgc gccttactac accaatgcgg tgagtcaggt aacacctgag
      721 ctggtcaaat aacaccaccg aaagatgcag atgctttctc agcatctgca tcatgtatta
      781 ca
//
LOCUS       DAZFXT010000379          775 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.382, whole genome shotgun sequence.
ACCESSION   DAZFXT010000379 DAZFXT010000000
VERSION     DAZFXT010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.382"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>775
                     /locus_tag="ACHKW0_005309"
     rRNA            <1..>775
                     /locus_tag="ACHKW0_005309"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          218 a          171 c          237 g          149 t
ORIGIN      
        1 cgggacacgt ggtatcctgt ctgaatatgg ggggaccatc ctccaaggct aaatactcct
       61 gactgaccga tagtgaacca gtaccgtgag ggaaaggcga aaagaacccc ggcgagggga
      121 gtgaaaaaga acctgaaacc gtgtacgtac aagcagtggg agcacgctta ggcgtgtgac
      181 tgcgtacctt ttgtataatg ggtcagcgac ttatattctg tagcaaggtt aaccgaatag
      241 gggagccgaa gggaaaccga gtcttaactg ggcgttaagt tgcagggtat agacccgaaa
      301 cccggtgatc tagccatggg caggttgaag gttgggtaac actaactgga ggaccgaacc
      361 gactaatgtt gaaaaattag cggatgactt gtggctgggg gtgaaaggcc aatcaaaccg
      421 ggagatagct ggttctcccc gaaagctatt taggtagcgc ctcgtgaatt catctccggg
      481 ggtagagcac tgtttcggca agggggtcat cccgacttac caacccgatg caaactgcga
      541 ataccggaga atgttatcac gggagacaca cggcgggtgc taacgtccgt cgtgaagagg
      601 gaaacaaccc agaccgccag ctaaggtccc aaagtcatgg ttaagtggga aacgatgtgg
      661 gaaggcccag acagccagga tgttggctta gaagcagcca tcatttaaag aaagcgtaat
      721 agctcactgg tcgagtcggc ctgcgcggaa gatgtaacgg ggctaaacca tgcac
//
LOCUS       DAZFXT010000380          768 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.383, whole genome shotgun sequence.
ACCESSION   DAZFXT010000380 DAZFXT010000000
VERSION     DAZFXT010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 768)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 768)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..768
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.383"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..462
                     /locus_tag="ACHKW0_005310"
     CDS             <1..462
                     /locus_tag="ACHKW0_005310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HFO7477925.1"
                     /translation="AINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEI
                     AKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEA
                     TDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
BASE COUNT          195 a          187 c          169 g          217 t
ORIGIN      
        1 gccatcaact tctcccgccc ggcgggtcag gtaattgcac agtactatga attcctgcgc
       61 ctcggtcgtg aaggctatac caaagtacag aacgcctctt accaggttgc cgcttatctg
      121 gcggatgaaa tcgccaaact ggggccgtat gagttcatct gtacgggtcg cccggacgaa
      181 ggcatcccgg cggtttgctt caaactgaaa gatggtgaag atccgggata caccctgtac
      241 gacctctctg aacgtctgcg tctgcgcggc tggcaggttc cggccttcac tctcggcggt
      301 gaagccaccg acatcgtggt gatgcgcatt atgtgtcgtc gcggcttcga aatggacttt
      361 gctgaactgt tgctggaaga ctacaaagcc tccctgaaat atctcagcga tcacccgaaa
      421 ctgcagggta ttgcccagca gaacagcttt aaacacacct gataacataa cgttgtaaaa
      481 accgaatgcc cagcctttaa aaaaacagct gggcattcgg ttgcttatta atcgcaataa
      541 tatattggct gtctattcat cgtgttgata agatatatgt tatatgaatg tttattaaat
      601 gtagcgattg cctttaatgt cacgaaaagg gattatttgc ttgctattaa tttcactgcg
      661 ttcttaaaca cgtttttgta ttataaatcc agtcatcctg cccgtagaac atcacacatt
      721 atcatcctgt tctcccgctt cgtttaaatt tatttatcaa tcaatttg
//
LOCUS       DAZFXT010000381          765 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.384, whole genome shotgun sequence.
ACCESSION   DAZFXT010000381 DAZFXT010000000
VERSION     DAZFXT010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.384"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..200
                     /locus_tag="ACHKW0_005311"
     CDS             <1..200
                     /locus_tag="ACHKW0_005311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO7477926.1"
                     /translation="LVSTIWGVFVRYQKWKKKFSDETNGKWREAIKKGMQYIKKINNN
                     KNINLYIKSKMQLYIISKLVS"
     gene            203..>765
                     /locus_tag="ACHKW0_005312"
     CDS             203..>765
                     /locus_tag="ACHKW0_005312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001298834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HFO7477927.1"
                     /translation="MKNLLITIIVASYNSKDYILETLESCINQQYQNIEIIIVDDCSS
                     DNSVELIKQWCKEKKSTSPKIRCILVESERNKGIPANLNNALPFINGDWIKCIGSDDI
                     LLPEAISEFVNRLEKCSNLDNIGAVFTYFQTFGYSVNVSTRYPSSWTRTICQMPPSWL
                     KKAAAMIHFNNVAPCAFINKNHFSGFDT"
BASE COUNT          301 a          104 c          122 g          238 t
ORIGIN      
        1 tccttgtaag tactatatgg ggagtgtttg ttcgttatca aaaatggaag aaaaaattca
       61 gtgatgaaac aaacggtaaa tggcgcgaag caataaaaaa aggaatgcaa tatataaaaa
      121 aaatcaataa caataagaat attaacctgt atattaaatc taaaatgcaa ttatatataa
      181 tatctaaact tgttagctaa atatgaaaaa tctattaatt acaattattg ttgcatcata
      241 taactcaaaa gattacatac tagagacatt agagtcatgc attaaccaac aatatcaaaa
      301 tattgagatt attatagtag atgattgctc tagtgataat agtgtagaac ttataaaaca
      361 atggtgtaaa gaaaagaaaa gcacatctcc taaaattaga tgtatattag tcgaaagcga
      421 aagaaataaa ggaattcctg caaaccttaa taatgctctt ccctttatta atggagactg
      481 gattaaatgt attggttcag atgatattct tcttcccgaa gcaatctctg agttcgtaaa
      541 tagattagaa aaatgctcaa atttggataa tataggtgca gtgtttactt atttccaaac
      601 ctttggatat tctgtaaatg ttagtactcg atatccatcc tcatggacaa gaactatatg
      661 ccaaatgcca ccatcatggt taaagaaggc agcggctatg attcacttta ataatgtggc
      721 accttgtgct tttataaata aaaatcattt tagtggattt gatac
//
LOCUS       DAZFXT010000382          763 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.385, whole genome shotgun sequence.
ACCESSION   DAZFXT010000382 DAZFXT010000000
VERSION     DAZFXT010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.385"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..737)
                     /locus_tag="ACHKW0_005313"
     CDS             complement(<1..737)
                     /locus_tag="ACHKW0_005313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFO7477928.1"
                     /translation="MIDFLNLKRINAQYEVELKDACSRVIDSGWYIMGTELKTFEEKF
                     ANWCGVKHVIGVANGLDALILVLRAWKEMGKLNDGDEIIVPANTYIASVLAITENNLV
                     PVLTEPDPLTFNLSAGGIRKVITGKTKAILPVHLYGQISPMAEIMEIAAEFGLLVLED
                     CAQAHGSSIDGRKAGSWGHAAGFSFYPGKNLGALGDAGAITTSDDQLADILTAIRNYG
                     SHEKYKNKYQGLNSRLDEMQAAFLNVK"
BASE COUNT          234 a          183 c          101 g          245 t
ORIGIN      
        1 aacttgacat tcaaaaatgc cgcctgcatt tcatccaatc gactatttaa gccttgatat
       61 ttgtttttat atttttcatg agaaccataa tttctaatag ctgtcaaaat atctgcaagt
      121 tgatcatcac tcgttgttat cgcacctgcg tcacctaaag cgccaagatt ttttcctgga
      181 tagaaactga atccagctgc atgaccccaa ctgccagctt tgcgtccatc gatagaggaa
      241 ccatgagctt gagcgcaatc ctctaaaacc aataaaccaa actctgccgc aatctccatt
      301 atttctgcca ttggagatat ttgtccatac aaatgcactg gcaaaattgc ttttgttttc
      361 cctgtaatta ctttacgaat cccacccgca cttaagttga atgtcaaagg atctggttca
      421 gtcaatacag ggactagatt attttcagtt attgccaaaa cagatgcaat atatgtattg
      481 gcaggtacaa taatttcatc tccgtcattt aatttcccca tttctttcca agcccgaaga
      541 acaaggatta gtgcatctaa gccattggca acaccaataa catgcttaac tccacaccaa
      601 tttgcaaatt tttcctcaaa tgtctttaac tccgtcccca taatatacca accagaatca
      661 ataactctgg agcaagcatc ttttaattcc acttcatatt gagcatttat tctttttaaa
      721 ttcaaaaaat caatcatttt ctttcccctc aaaaatattt act
//
LOCUS       DAZFXT010000383          757 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.386, whole genome shotgun sequence.
ACCESSION   DAZFXT010000383 DAZFXT010000000
VERSION     DAZFXT010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.386"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..669
                     /locus_tag="ACHKW0_005314"
     CDS             <1..669
                     /locus_tag="ACHKW0_005314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HFO7477929.1"
                     /translation="DARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQE
                     LNRTETAREVQRPSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGE
                     KTSAVLLAYIGLKDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALY
                     MPAMVATSKTEWGRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRHN
                     PVAA"
BASE COUNT          184 a          189 c          246 g          138 t
ORIGIN      
        1 gatgcgcgca tgctggcaga gttctgtcgt cagaagcgcc ctgcagcctg ggaagcgcct
       61 cacccgcttg aacgcgcgtt gcgtgccctg gtagtccgcc accaggcgct gacagatatg
      121 cacacgcagg aactgaatcg cactgaaacg gcgcgggaag tccagagacc gagcattgat
      181 gctcaccttc tgtggcttga agcagagctg aagcgtcttg agaagcagat aaaagacctg
      241 acagacgatg atccggatat gaaacaccgc aggaaactgc tggaaagcat cccgggtatc
      301 ggagagaaaa catctgcggt attgctggct tatatcggtc tgaaggaccg cttcgcccat
      361 gccagacagt tcgccgcttt tgcgggtctg acaccacggc gttatgaatc aggtagcagt
      421 gtgagagggg cgagccggat gagtaaggcc ggacatgtgt cgcttcgcag ggcgttgtat
      481 atgcccgcaa tggtagccac cagtaagact gagtggggac gggcgttccg cgaccgtctg
      541 gcggctaatg gcaagaaagg aaaggtgatt ctcggcgcga tgatgcgcaa gctggcacag
      601 gtggcgtatg gagtgctgaa gtcaggcgtg ccgttcgatg cgtcacggca taatccggta
      661 gcggcgtaaa aatcgcggaa gggatgaaaa aaacagcgcc tgacggcgct gtgtctggca
      721 tgcctgcaat ccgggaaacc ggaccaggaa aaaactt
//
LOCUS       DAZFXT010000384          751 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.387, whole genome shotgun sequence.
ACCESSION   DAZFXT010000384 DAZFXT010000000
VERSION     DAZFXT010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.387"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            129..751
                     /locus_tag="ACHKW0_005315"
                     /pseudo
     CDS             129..751
                     /locus_tag="ACHKW0_005315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
BASE COUNT          260 a           97 c          152 g          242 t
ORIGIN      
        1 aaaaatttta tttttaattg tatttcatta ttatttcatc aatgtattct ttggattttg
       61 cttataataa taatggaagg gatgattata aatgaatgcg ttaaagcatt tacactcagg
      121 atgaatatat gggaaaaatt aaaattgtgg tttcagatca gcagccgttt atgattgatg
      181 ggataattgg atttctcgga cattatcctg atttatataa ggttgttggg ggctataaag
      241 atctgaagaa agctatagcg gagtgtaaca aatctacagc ctaaatattt attttaggag
      301 aatttgcagg tggaatgatg gggtccgaac tcgtaaaatg ggttaaatcg cataagatag
      361 atgctcatat tattacattt gtagcaaaga tgccgtatat tgattcaata aaattgcttg
      421 aagcaggtgc gaaaggatgc gtatggaaaa ccagtcaccc ggcgaaacta aatcgtgcta
      481 ttgattcgat tagtaatggt tacacttatt ttgatagtgt acatatggat tgtgaaaaaa
      541 tatcttccag gtattcctct gataatcaac ttacaaatcg tgagtcagaa atattacaac
      601 tcatagctga tggtaaaaca aacaaagaaa tcgctaattt cttgcagtta agcagaaaaa
      661 cagtcgaaac tcatagactc aatatcatga agaaattaga tgttcacagt ggtatcgagt
      721 tgatcaaaac agctttacgt atgggtgttt g
//
LOCUS       DAZFXT010000385          741 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.388, whole genome shotgun sequence.
ACCESSION   DAZFXT010000385 DAZFXT010000000
VERSION     DAZFXT010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.388"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..308)
                     /locus_tag="ACHKW0_005316"
     CDS             complement(<1..308)
                     /locus_tag="ACHKW0_005316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HFO7477930.1"
                     /translation="MLHDHVAECLEKKGLYRRAAERWAKVMVQLSDDQKRKVAAQKRA
                     ECLRKARRTPVSPVNLTEIKQAVNRLHSELGMGFEERRVFRRYKGTGEQNTSGNARSK
                     "
BASE COUNT          214 a          193 c          104 g          230 t
ORIGIN      
        1 ttttttgacc gcgcgtttcc ggacgtattc tgttctcctg tccctttata tcgtcggaat
       61 acccgccgct cttcaaatcc cattcccaac tcagaatgta gtctgttgac cgcttgtttt
      121 atttcggtca ggttcaccgg tgaaaccgga gtccggcgcg ccttacgcaa acactctgct
      181 cgtttctgtg ccgccacttt tcttttctgg tcatcactta gctgtaccat cacttttgcc
      241 catcgttcag ctgctctccg gtacagtcct tttttctcca gacattctgc cacgtgatca
      301 tgtagcataa gtgacctccg attatctaca gactgccatc ctgaatttac cttcccttaa
      361 tgaaataaca ataaaaaaca aaccacgcaa aaacaataaa acaacacaca aaaaaaacta
      421 aataataaac aaaaataatc accttatttt attatttttt gagggagcaa ttactgaaca
      481 aaaaacgctg actatatact caaaaccaaa caactattct gccaatcagg tatcatggca
      541 acacacggaa ttaccgtgtt tttgccttct ctgcccatac aatacgggca tatacttcat
      601 tctctattgt aatatttcta tccatgtgcc ccactccatt tacctgtaaa taatattcaa
      661 aatatttatc acagaaatcg tttttggcca tgaactgagc acactataaa gtccggaact
      721 gactctttgt taaattacct t
//
LOCUS       DAZFXT010000386          737 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.389, whole genome shotgun sequence.
ACCESSION   DAZFXT010000386 DAZFXT010000000
VERSION     DAZFXT010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 737)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..737
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.389"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>737)
                     /gene="wzx"
                     /locus_tag="ACHKW0_005317"
     CDS             complement(<1..>737)
                     /gene="wzx"
                     /locus_tag="ACHKW0_005317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_099459345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O103 family O-antigen flippase"
                     /protein_id="HFO7477931.1"
                     /translation="IFLGVLNGLNEYNRFFLANVLAISSSLLSMVGLVYFFGLKGALV
                     SASLNNAVAGVWLITIIIKRPWFKFKYWVGHTPRHNITQMKNYFYMGVIGALTGPISM
                     IVVRTILTNNFSLEDAGYWQAVNRISEAYLAVLTTALTVYYFPKTAAARRYSEYITLL
                     KTGACIVVPLALSMALTIYGLKDFIISILFTADFIRARELFLFQNIGDFLRICSWLFA
                     TILLAKGYFKINAILEVMFSIIFPVLV"
BASE COUNT          277 a          153 c          108 g          199 t
ORIGIN      
        1 tttaccaata ctggaaaaat tattgaaaac atcacctcca atatagcatt tattttaaaa
       61 tatccctttg ctaacaatat agtggcaaac agccaactac aaatccgcag aaaatccccg
      121 atattttgaa ataaaaaaag ctcccgagca cgtataaagt cagccgtgaa caagatacta
      181 attataaaat ctttcaagcc gtagatggtt aaagccatgc tcaacgcgag cggtacaaca
      241 atacaagccc cggtctttaa aagagttata tattcgctat atcttcttgc ggctgcagtt
      301 tttggaaaat aataaactgt caatgccgta gtaagaacag ccagataagc ctcagaaatt
      361 ctatttacag cttgccaata tccggcatcc tctaaagaaa aattattagt gagaatagtt
      421 cgaacaacta tcatcgaaat aggtccagtt aacgctccaa tcacccccat ataaaagtaa
      481 tttttcatct gagtaatgtt atgccgagga gtatgtccca cccagtattt aaatttaaac
      541 catggtcttt taattataat agtaataagc cagacacctg caaccgcatt atttaatgat
      601 gcagacacta atgcgccttt gaggccaaaa aaataaacta agcccaccat agataacaac
      661 gatgaggata tagctaaaac attcgcaagg aaaaatctat tatattcatt tagtccattt
      721 aaaaccccta aaaatat
//
LOCUS       DAZFXT010000387          735 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.390, whole genome shotgun sequence.
ACCESSION   DAZFXT010000387 DAZFXT010000000
VERSION     DAZFXT010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..735
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.390"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..486
                     /gene="excA"
                     /locus_tag="ACHKW0_005318"
     CDS             <1..486
                     /gene="excA"
                     /locus_tag="ACHKW0_005318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HFO7477932.1"
                     /translation="AYGSWTLLIIPSLWLYLKTRAKKKKILQVVKKIKESSPFAPTSD
                     YEQLSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTY
                     VSLPMIPLEISGISSRTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEV
                     F"
     gene            complement(551..>735)
                     /locus_tag="ACHKW0_005319"
     CDS             complement(551..>735)
                     /locus_tag="ACHKW0_005319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001302633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HFO7477933.1"
                     /translation="TFLFEMMKDKSKDVTLAVIYALGEGRCQADNIKRELRRLSESDD
                     MEIKVAAIKALGRLYR"
BASE COUNT          214 a          177 c          146 g          198 t
ORIGIN      
        1 gcatatggaa gttggacgtt actcatcatc ccatcactct ggctatatct gaaaaccaga
       61 gcgaagaaaa agaaaatcct gcaggttgtc aagaagatca aagaaagcag tccctttgcg
      121 ccgaccagcg attacgagca actgtcattc tcgaagagtt gttatttcgg gatcgacatt
      181 aaaaacggca ccatgctgta tgtgcgcatt tacccaaaca atgtcatgga tgtgattggc
      241 ctggatattc acaacttcac ccgaacagtt gctgaagacg gcaagctgga aatccacacc
      301 acctatgtga gtctgccgat gatccctctt gaaatctccg gcatctcatc aaggacactg
      361 gccaacacca tgcacaccat ggctgcacgt ggatatgagt acaaggaacg tttcccgcag
      421 atgattcggt atcgggttaa agaatgggaa aaagttgccg gtgtgcctgt tgcagaagtg
      481 ttttaatacg gctcatacat ccggttcatc tgaaccggat tttctttact acaacagata
      541 acttatatcg ttatcgataa agtcgcccaa gagccttgat agctgcaact ttaatctcca
      601 tatcatcact ttcacttaat ctacgtaatt ccctctttat gttatctgcc tggcatcgac
      661 cttcacctaa tgcatagatc acagcaagtg taacgtcttt cgatttatcc ttcatcattt
      721 caaacaagaa agtat
//
LOCUS       DAZFXT010000388          726 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.391, whole genome shotgun sequence.
ACCESSION   DAZFXT010000388 DAZFXT010000000
VERSION     DAZFXT010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..726
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.391"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>726
                     /locus_tag="ACHKW0_005320"
     CDS             <1..>726
                     /locus_tag="ACHKW0_005320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7477934.1"
                     /translation="GGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLNDPAYRRLR
                     IITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGDQFDTVEVDFGRSEGNNIEQADGK
                     KWSEQDRDTFDPTHDIDLYCDQASGLVNIAIMDGTVWRLLNGFKLFREKLDTRRGSNS
                     QLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPEGTLVLGNTAADGIR
                     CYGAIQDAQALSEGVVASSRYPKH"
BASE COUNT          175 a          167 c          218 g          166 t
ORIGIN      
        1 ggtggtgaaa cccgcgtgtt acgtccgggc tacgtcaagc cgaaacacga atttaattac
       61 cagcaggcgg ttgagcgtct tcccggtgaa gatccggctc aactgaacga cccggcctac
      121 cgtcgtctgc gtatcatcac cgataacctc aaacaggaag agcatgccat tgtccaggtg
      181 gaagaaatgc aggcggtgaa tgccgtgctg tatggcaaat acaccatgga aggggatcag
      241 tttgatactg tcgaggtgga ttttggacgc tctgaaggaa ataacattga gcaggccgac
      301 ggtaaaaaat ggtctgagca ggaccgtgat acgtttgatc cgacgcatga tattgacctc
      361 tactgcgatc aggccagcgg tcttgtgaat attgccatta tggacggtac tgtctggcgt
      421 ctgctgaatg gttttaagct gttccgcgaa aaactggata cccgtcgcgg ctcaaattca
      481 caactcgaaa cggcagtgaa agacctgggg gcggtggtgt ctttcaaagg gtattacggc
      541 gatctggcca ttgtggtggc gaaaacgtct tatgtggcag aggacggtac cgaaaaacgt
      601 tatctgccgg agggcacgct ggtcctggga aatacggctg cagatgggat ccgttgttac
      661 ggtgccattc aggatgcgca ggcgttgtcc gaaggtgtgg tggcttcttc ccgttacccg
      721 aaacac
//
LOCUS       DAZFXT010000389          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.392, whole genome shotgun sequence.
ACCESSION   DAZFXT010000389 DAZFXT010000000
VERSION     DAZFXT010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.392"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>722)
                     /gene="tssI"
                     /locus_tag="ACHKW0_005321"
     CDS             complement(<1..>722)
                     /gene="tssI"
                     /locus_tag="ACHKW0_005321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HFO7477935.1"
                     /translation="DQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSAQIR
                     PSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKSAHGQNFARWQMDG
                     WRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGRQGA
                     GTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKFNWD
                     RYNPADQDSSCWIRVAQAWAGTG"
BASE COUNT          109 a          235 c          213 g          165 t
ORIGIN      
        1 ccggtgcctg cccacgcctg tgccacacgg atccagcacg aactgtcttg gtctgccggg
       61 ttataacggt cccagttgaa cttcacccgc acgcgaccat gttcgtcgca gaagatttcc
      121 tcgcctgccg gtcctgtcac gacagcgctc tgcgggccgt cgaccagcgg tttcagcaac
      181 ggctgtggtc gccatgttct gtctgccggg atcaccgcaa aatggttctc cagcgccgtc
      241 cccgctccct gccgtcctgg caccgcctgt ggctgttcgc cgtgcagttc acttgccacc
      301 acctgccatt cccggttcag gttcgcctgc ggatgccccg tcagcacaat tcgccgtccc
      361 ggccatatct ccggcgagcg gctcattccc cgcgcggttt ctgcgttgtt tcgccagccg
      421 tccatctgcc agcgggcaaa gttctgcccg tgggcgctct tgaaacgtcc ggggtagtca
      481 tacacttcat actgcgtgcg ctggtagtcc tggtgctggc cttcctgttc aaaacgtccg
      541 gcccagccgg ggcgtttaaa ggtgtagtct ttggtcacca cggaagaagg gcggatttgt
      601 gcgctgtagc ggaactggct gatgcagagg gtgctcacct cggtacgggt gttcggattc
      661 caggggattt caaaagattc gggcagatgg cggactgtgt cgcacagcac caggctctgg
      721 tc
//
LOCUS       DAZFXT010000390          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.393, whole genome shotgun sequence.
ACCESSION   DAZFXT010000390 DAZFXT010000000
VERSION     DAZFXT010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.393"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>722
                     /locus_tag="ACHKW0_005322"
     CDS             <1..>722
                     /locus_tag="ACHKW0_005322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HFO7477936.1"
                     /translation="VKLDERETFNVSWGIDENYQVGAAISIASILENNKQNKFTFHII
                     ADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQSNIWPVSIYYRLLSFDYFSA
                     RLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDAMQSKSAERLCNEDFNGSYF
                     NSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQDILNLMFLHHAKILPRKYN
                     CIYTIKSEFEEKNSEYYTRFIN"
BASE COUNT          253 a           95 c          128 g          246 t
ORIGIN      
        1 ccgttaaact tgatgaacgg gaaacgttta atgtctcatg gggtattgat gagaactacc
       61 aggttggggc tgcgatttca attgcttcaa ttcttgaaaa taataaacaa aacaaattta
      121 cctttcacat aatcgctgat tacttagaca aagagtatat tgaattatta tcacaattag
      181 caacgaagta tcaaacagta attaaattat atcatattga ttctgagcca ttgaaggcgc
      241 tacctcaatc aaatatctgg ccagtatcta tttattatcg tttgctttca tttgattatt
      301 tttctgcgcg attggattca ttattatatc ttgatgctga tatcgtctgt aagggttcat
      361 tgaacgagtt aatagcatta gagtttaaag atgaatatgg ggcagtggta attgatgtag
      421 atgctatgca aagtaaaagc gctgagcgtt tgtgtaatga ggattttaac ggtagctatt
      481 ttaactctgg tgtaatgtat attaatttac gggaatggtt aaaacaaaga ctaacggaaa
      541 aattctttga tctattatca gatgagtcaa ttataaaaaa attaaagtac ccggatcaag
      601 atattttaaa cttaatgttt ctacatcatg ctaaaatatt accgagaaaa tataattgta
      661 tttatactat aaagtcagaa tttgaagaaa aaaatagtga atattacacc cggtttatta
      721 at
//
LOCUS       DAZFXT010000391          722 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.394, whole genome shotgun sequence.
ACCESSION   DAZFXT010000391 DAZFXT010000000
VERSION     DAZFXT010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..722
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.394"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..645
                     /locus_tag="ACHKW0_005323"
     CDS             <1..645
                     /locus_tag="ACHKW0_005323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7477937.1"
                     /translation="RLPELLSRIDGYKGQPVTRLAVMLNLLVFIRSSELRYARWSEID
                     IDNAMWTIPAEREPLPGVKFSHRGSKMRTPHLVPLSKQAVAILTELQTWAGENGLIFT
                     GAHDPRKPISENTVNKALRVMGYDTTQEVCGHGFRAMACSALIESGLWSRDAVERQMS
                     HQERNGVRAAYIHKAEHLEERRLMLQWWADFLDANREKFISPFEYAKINNPLKQ"
BASE COUNT          208 a          164 c          173 g          177 t
ORIGIN      
        1 cgcctgcctg aacttctgtc gcgtattgac ggttataaag gccagcctgt cacccgactg
       61 gcagttatgc tgaatttact ggtttttatt cgttccagtg aactcagata cgcccgctgg
      121 tctgaaattg atattgacaa tgccatgtgg actattccag ccgaacgcga acctctgcca
      181 ggcgtaaaat tctcacaccg gggctccaag atgcgaacac cacatcttgt gccactcagc
      241 aaacaggctg tagccatact gacagaactt cagacatggg caggtgaaaa tggtctgata
      301 tttacgggtg cacatgaccc gcgtaaacca atcagtgaaa atactgtaaa taaagccctg
      361 agggtgatgg ggtatgacac aacccaggaa gtctgtggcc atggattccg ggcgatggcg
      421 tgcagtgcat tgattgaatc aggtttgtgg tcccgcgatg ctgtggaacg tcagatgagc
      481 catcaggaac gtaacggtgt acgtgctgct tacattcata aagcagaaca tctggaagaa
      541 cgccgcttga tgctacaatg gtgggccgat tttctggatg caaacagaga aaaatttatc
      601 agtccatttg aatatgcaaa gattaataat ccattaaaac agtaatcatc ccgggcaaat
      661 gcccgggaac ctactccagg attattttct ttgttaaaaa aacagacaaa acggtattaa
      721 ct
//
LOCUS       DAZFXT010000392          720 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.395, whole genome shotgun sequence.
ACCESSION   DAZFXT010000392 DAZFXT010000000
VERSION     DAZFXT010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.395"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..606)
                     /locus_tag="ACHKW0_005324"
     CDS             complement(<1..606)
                     /locus_tag="ACHKW0_005324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000941575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477938.1"
                     /translation="MLQRDPHGFRKDVKEFITMIKSFQVDIRTENNLTSAYKHVLFLE
                     FIHNSHPEISHKTFIKSIIYDVLSSITAILHKRERYLHLNFRSMIEHLARISLQKVDN
                     GGDFDITIRTLDFENLKSTNTDENWAYMHSQYKQACGWLHSSSKVKLNITASFPDLLK
                     SDSKSNAAKLSTHLQKMTTEMLKIFFNYYVDEIQTSFYRTRG"
BASE COUNT          222 a          112 c          122 g          264 t
ORIGIN      
        1 tccccgtgtg cggtaaaaag aagtttgaat ctcatcaaca tagtagttaa aaaaaatctt
       61 taacatttcg gtagtcatct tttgcaaatg ggttgacagt ttagctgcat tactttttga
      121 atcagacttt aataaatctg gaaatgatgc tgtgatattt aatttaactt tagatgatga
      181 gtgtaaccag ccacatgctt gtttatactg tgaatgcatg tacgcccagt tttcatcagt
      241 gttggtagat ttaagattct cgaaatcaag agttctaatt gtaatatcga aatccccccc
      301 gttatcaact ttttgtaagg atatacgtgc aagatgttca atcattgagc gaaaattaag
      361 atgaagatat cgctctcgtt tatgaagtat cgctgtaatt gaacttaaaa catcataaat
      421 aatacttttt ataaaggttt tgtgacttat ttctggatgt gagttatgga taaactccag
      481 aaaaagtaca tgtttataag ccgatgtaag attgttttcg gtgcgaatat cgacctgaaa
      541 tgattttatc attgtgataa attctttcac gtcttttcta aatccatgtg ggtctctttg
      601 aagcattatt ttctatcctt cttcaaaaga gcattaatcc acgtacctac atctcgattt
      661 gcattacgtt tgacgttact ttttttaaaa gtaagtgagt tttcattata tccaataata
//
LOCUS       DAZFXT010000393          719 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.396, whole genome shotgun sequence.
ACCESSION   DAZFXT010000393 DAZFXT010000000
VERSION     DAZFXT010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.396"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            72..>719
                     /locus_tag="ACHKW0_005325"
     CDS             72..>719
                     /locus_tag="ACHKW0_005325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HFO7477939.1"
                     /translation="METVSDALKALKKASSHVVAARLGISREEAVNELWELKRNGVVD
                     KTGHTWFLAGEGESRVTEERPVKSEAQDMLTGEVEQKVTADMMIEFIGQDGAKTCEEL
                     ADKFGVSIRKVASTLAVVTATGRLARVNQNGKFRYCMPGGNLPADPKAAPVTENDGKA
                     FPQPAGAALPVREAATQEEIKTETVADIVQPLPSFTETQADELIFPSLRRANLALR"
BASE COUNT          196 a          157 c          213 g          153 t
ORIGIN      
        1 actgggatag ctggcgtcca aatgtcagca atatgagggt tgtgaagtaa ttttgtccgg
       61 aggaaatttt aatggaaacc gtatctgacg cactgaaagc actgaaaaaa gcctcttcac
      121 atgtggtggc agctcgcctt ggaatcagtc gtgaagaggc tgtcaacgag ctgtgggaac
      181 tcaaaagaaa tggcgtcgtt gataaaactg gtcacacctg gtttctggct ggcgaaggtg
      241 aatcccgggt aaccgaagag cggccagtaa aatctgaagc acaggatatg ctgaccgggg
      301 aggtcgaaca aaaagttacc gcagacatga tgattgagtt tatcggtcag gatggggcta
      361 aaacgtgtga ggaactggcg gataagttcg gtgttagcat tcgcaaggtt gcttccacgt
      421 tggcggtcgt aacagcaacg gggcgcctgg cacgcgtaaa tcagaacggt aaatttcgtt
      481 actgcatgcc ggggggtaat ttaccagcag atccgaaagc cgcgccggta acggaaaatg
      541 atggtaaggc ctttcctcag ccagcaggtg ctgcgttacc agtccgggaa gccgcaacac
      601 aggaagaaat taaaacagaa actgtggcgg acattgtgca gccgttgcca tcgtttaccg
      661 aaacgcaagc agatgagctg atttttccgt cccttcgcag ggcaaacctg gcgctgcgc
//
LOCUS       DAZFXT010000394          712 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.397, whole genome shotgun sequence.
ACCESSION   DAZFXT010000394 DAZFXT010000000
VERSION     DAZFXT010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.397"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..72
                     /locus_tag="ACHKW0_005326"
     CDS             <1..72
                     /locus_tag="ACHKW0_005326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO7477940.1"
                     /translation="LLTYLTNLYFKIREDRRKAARGE"
     gene            77..610
                     /locus_tag="ACHKW0_005327"
     CDS             77..610
                     /locus_tag="ACHKW0_005327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HFO7477941.1"
                     /translation="MSRKLRYGLSAAVLALIAAGASAPEILDQFLDEKEGNHTTAYRD
                     GAGIWTICRGATRVDGKPVVPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYRRINAGDRKGACEAIRWWIKDGGRDCRIRSNNCYGQ
                     ISRRDQESALACWGIDR"
BASE COUNT          192 a          154 c          209 g          157 t
ORIGIN      
        1 ctgctgacgt acctgacgaa cctgtatttc aaaatcagag aggacaggcg taaggcggca
       61 cggggagagt aagctgatga gcaggaaact ccgctatggt ttatcggctg ccgttctggc
      121 gctgattgcc gcaggtgctt ctgcgcctga aatcctcgac cagtttctgg atgaaaagga
      181 aggtaaccac accacggcat accgtgatgg tgcgggtatc tggaccatct gccgtggagc
      241 cacccgagtg gatggtaagc ctgtcgtccc gggtatgaag ttatcgaagg aaaagtgcga
      301 ccaggttaac gctatcgaac gggataaggc gctggcatgg gtggagcgta atataaaagt
      361 tccactgacc gagccacaaa aagcaggtat cgcgtcattc tgtccgtaca acattggtcc
      421 cggtaagtgt ttcccgtcga cgttttacag acgaattaat gcaggtgatc gaaaaggtgc
      481 ctgcgaagcg attcgctggt ggattaagga cggcggcaga gactgccgta ttcgttcaaa
      541 caactgttac ggtcagatat cccgtcgtga ccaggagagc gcgctggcgt gctggggaat
      601 cgacagataa gaagaatatt ttgctgaaaa atgaggtttg cttacatgga cggataacac
      661 gaaatcctgc gaactggcaa aatgtaagtg aataaagtca acaagattgt tt
//
LOCUS       DAZFXT010000395          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.398, whole genome shotgun sequence.
ACCESSION   DAZFXT010000395 DAZFXT010000000
VERSION     DAZFXT010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.398"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            7..432
                     /locus_tag="ACHKW0_005328"
     CDS             7..432
                     /locus_tag="ACHKW0_005328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7477942.1"
                     /translation="MELQDWRKEPRKKYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGHVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTMLIRELSGRGR"
     gene            429..>710
                     /gene="tnpB"
                     /locus_tag="ACHKW0_005329"
     CDS             429..>710
                     /gene="tnpB"
                     /locus_tag="ACHKW0_005329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311895.1"
                     /note="TnpB, as the term is used for proteins encoded by
                     IS66 family insertion elements, is considered an accessory
                     protein, since TnpC, encoded by a neighboring gene, is a
                     DDE family transposase; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HFO7477943.1"
                     /translation="MISLPSGTRIWLVAGVTDMCKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLA"
BASE COUNT          178 a          171 c          185 g          176 t
ORIGIN      
        1 ctaagtatgg aattacagga ctggcgaaaa gaacctcgta aaaagtattc gaatgaattc
       61 aaacttcgta tggttgaact ggcatcacaa cctggagctt gtgttgcaca gattgcacga
      121 gaaaatggcg tcaatgataa tgttattttc aaatggctca ggctctggca gaacgaaggg
      181 catgtttcgc ggcgtcttcc ggtaacaacc tcttctgaca ctggcgttga attattacct
      241 gtagaaataa cgccggatga gcagaaagaa cctgtggcgg ccattgcgcc gtctttatcc
      301 acttccactc agaccagagt cagtgccagt tcctgcaagg tggaattccg tcacggtaac
      361 atgacgctgg aaaatccatc gccagagctg ctcacgatgt taatccgcga actgagcggg
      421 aggggaagat gatttcactc ccatcaggta cccgtatctg gctcgttgcc ggcgttaccg
      481 atatgtgtaa atccttcaac ggactgggag aacaggtaca acatgtgctg aatgataatc
      541 ccttctccgg tcacctgttt atcttccgtg gccgacgggg ggacaccgtt aaaattcttt
      601 gggctgatgc tgatggtctg tgcctgttca ccaaacgcct ggaggaaggc cagtttatct
      661 ggcctgcggt gcgtgacggt aaggtatcca ttacccgctc gcaactggca
//
LOCUS       DAZFXT010000396          710 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.399, whole genome shotgun sequence.
ACCESSION   DAZFXT010000396 DAZFXT010000000
VERSION     DAZFXT010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.399"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            196..>710
                     /locus_tag="ACHKW0_005330"
     CDS             196..>710
                     /locus_tag="ACHKW0_005330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HFO7477944.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAV"
BASE COUNT          213 a          126 c          158 g          213 t
ORIGIN      
        1 gatagtcatt agatgttgaa tcaatgaatt actcgttcag ttaatattta aatattcatg
       61 aaatctataa attaaagatt tgtcacttat tggatttagt atgcttgatt taaatggcgg
      121 tcaattattg ttattattaa gtctaatgtc atttaaaggc attatcatat ttacgaagag
      181 atcaggatga tgacgatgaa aaaaagtgta ttgacggcgt ttataactgt ggtatgtgca
      241 acgtccagcg ttatggcagc tgatgataat gctatcacgg atggcaaagt aacatttaat
      301 ggtaaagtta ttgctccagc ttgtaccctg gtagctgcga cgaaagattc agtagtcaca
      361 ttgccaaatg tcagcgccac caagttgcaa actaatggag cagtctctgg cgttaaaact
      421 gatgtaccaa ttgccttaga aggctgtgat gttactgtaa ctaaaaatgc gacgtttacc
      481 tttagtggta cggctgacgg cgtgcaacct acagcatttg ccaaccaggc taccaccgat
      541 gcagcgacga atgttgctct acaaatgtat ctgccagatg gttctacatc ggtaactccc
      601 ggtaccgaaa cgtcgaatat tcagttagcg gattctgctg agcaaacagt gacctttaaa
      661 gttgattata tcgctacggg gaaagcgact tctggtaatg tgaatgcggt
//
LOCUS       DAZFXT010000397          707 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.400, whole genome shotgun sequence.
ACCESSION   DAZFXT010000397 DAZFXT010000000
VERSION     DAZFXT010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.400"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>707
                     /gene="espX1"
                     /locus_tag="ACHKW0_005331"
     CDS             <1..>707
                     /gene="espX1"
                     /locus_tag="ACHKW0_005331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO7477945.1"
                     /translation="CLYKSNIVDDVIINKTRQNELVKVFCEYKTEFLILFDDFFRSHN
                     LPKPSPVLHHFFQYTHLRDAYFYRCKLIEHTVQFYFFKHKGITLLRLDVFDDRTSECL
                     SEEIKIYQECHEKFVNFLKANFNQEIYPQLYTPEIFYEACRNLQSFYDHQETSQNAKY
                     SAIDKKKSHLNQEIKKLIKKNIFPELYNEQCNKIPSSSIDDNQGIMWQNFKTSNIAYS
                     QLCKKLSLFKSIPSRLI"
BASE COUNT          281 a          114 c           95 g          217 t
ORIGIN      
        1 tgtctttata aatcaaatat agtagatgat gtaataataa ataaaacaag acaaaatgag
       61 ttagtaaaag tattttgcga gtataaaaca gagttcttaa ttttgtttga tgactttttt
      121 agaagccata acttgcctaa accatctcca gtccttcatc atttttttca atatactcat
      181 ctgagagatg catattttta tagatgtaaa ctcatagaac atacagttca attttatttt
      241 tttaaacaca aaggtatcac attacttaga ttggatgttt ttgatgacag aacaagcgaa
      301 tgtctttctg aagagattaa gatataccaa gaatgccatg aaaaattcgt taatttttta
      361 aaggcaaact tcaaccaaga aatataccca cagctatata ctcctgaaat attttatgag
      421 gcctgcagaa atctccagtc attttatgat catcaggaaa cgtcacaaaa cgcaaaatac
      481 agtgcaatag acaagaaaaa aagtcatctt aaccaggaaa tcaaaaagct tattaaaaaa
      541 aatattttcc ctgagctata caatgagcaa tgcaataaaa tcccctcctc atcgatagat
      601 gataaccagg gaataatgtg gcagaacttt aaaactagca atattgcata ttcacaacta
      661 tgtaaaaaat tatctttatt taaaagcata cccagtcgat taataaa
//
LOCUS       DAZFXT010000398          702 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.401, whole genome shotgun sequence.
ACCESSION   DAZFXT010000398 DAZFXT010000000
VERSION     DAZFXT010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.401"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..467)
                     /locus_tag="ACHKW0_005332"
     CDS             complement(<1..467)
                     /locus_tag="ACHKW0_005332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HFO7477946.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYD"
BASE COUNT          192 a          172 c          177 g          161 t
ORIGIN      
        1 aaatcgtaat cagtctcacc gtactggaca caaaactcac gggaaggatg cggctcgctg
       61 aacagcggac tccattcggt caccccgttt tcctgcaaca tcgtgccgag gatgcttttg
      121 atgtcctcgt tctggaagat acggaagttc tggcgcagac cggcacgcca cagcggcggg
      181 tgcaccttca tgctgtacag catctggttt ttgtcgttct ccccgagttc aaaccaggtc
      241 accacgcctt tcacccggcg ctgtacgtca tcgccctgcc atatcgtcag gtaggccatt
      301 ttgtccagca cctgcgcaaa ttcaagggag agaaactgct ggctgaccag ggagagatcg
      361 agggaaaaga gcgaagagag tgactggttc agatgaaagg aaaccaccgc aaaagcgtcc
      421 ggcggcaggc cgtccacttc cagtgtgaaa cgtaatccgg ttgacatagc aacctccagt
      481 aaaatgaata atgatccgta ccgtacaact gcggataatg tgataagcgg gacgacttca
      541 gacgttatta gggaaggaac agaaaatgct atggttatgt gagaattttg tttaaatatt
      601 actttacaga aacgggtgtc tgttcagtca aaaaaataat cacaagctca acctcttgtt
      661 tcttggcgtt attcaggaca ggtacgcata tcacaacgag ta
//
LOCUS       DAZFXT010000399          691 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.402, whole genome shotgun sequence.
ACCESSION   DAZFXT010000399 DAZFXT010000000
VERSION     DAZFXT010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 691)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 691)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..691
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.402"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..366)
                     /locus_tag="ACHKW0_005333"
     CDS             complement(<1..366)
                     /locus_tag="ACHKW0_005333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HFO7477947.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANATTYDTWTYYDNPTTALD
                     WSNMDAPGTVDGNYVNYSGFVYYNNTNGDFDTTFNGDTVNGTISTYYLNHDFTDGTTN
                     QLNISNSLIHGSITSMLPMG"
BASE COUNT          225 a          128 c          132 g          206 t
ORIGIN      
        1 acccataggc agcattgaag tgattgaacc atgaatcagg gagttgctga tgttcaactg
       61 gtttgttgtt ccatcagtaa agtcatgatt caggtaatat gtagagatag tcccgttaac
      121 agtgtcacca ttaaaggtgg tatcgaaatc accgtttgtg ttgttatagt agacaaagcc
      181 actataattc acatagttac catctacagt acccggtgca tccatattgc tccagtcaag
      241 agcggtagtt ggattgtcgt aataagtcca ggtgtcataa gtagttgcat tagcctgaga
      301 ggtgatagcc agagtgcaag ccaatgctaa ttgtgatact acaagtttct ttttccagga
      361 gtgcatttgt catccctcct cagggacgta ataagttgat ccatcaatta tcaatgcata
      421 gaaaacatat tatgtttcgc gctaatgatt atgctgcgtt acagacgaat ggttcaatta
      481 ttctttcccc taagtccgaa tgtggacata tataacctat ggagaaaaac acgcatcaaa
      541 ttgataaaag ggcgatattt caacatgttg cagataatgt tatttttgat acatacgcgt
      601 tataaatatc aaaaacacaa accagataaa tatcttatca atatacaaca aagtttgaat
      661 tattcaattt atagcagcac aaccaaacgc c
//
LOCUS       DAZFXT010000400          686 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.403, whole genome shotgun sequence.
ACCESSION   DAZFXT010000400 DAZFXT010000000
VERSION     DAZFXT010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.403"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>686
                     /locus_tag="ACHKW0_005334"
     CDS             <1..>686
                     /locus_tag="ACHKW0_005334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7477948.1"
                     /translation="RQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAATKGFDDQTRR
                     LKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKKADDIWSLRKD
                     DYFVNDEARARYWDDREKARLALEAARKKAEQQSQQDKNAQQQSDTEASRLKYTEEAQ
                     KAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEATLKKPSAVKVS
                     AGERQEDRAHA"
BASE COUNT          192 a          157 c          225 g          112 t
ORIGIN      
        1 cgccagttcc ataacgtgac ggcggagcag attgcgtatg ttgctcagtt gcagcgttcc
       61 ggcgatgaag ccggggcatt gcaggcggcg aacgaggccg cgacgaaagg atttgatgac
      121 cagacccgcc gcctgaaaga gaacatgggt acgctagaga cctgggcaga caggacagca
      181 cgggcattca aatccatgtg ggatgcggtg ctggatattg gtcgtcctga taccgctcag
      241 gagatgctga ttaaggcaga ggctgcgttt aagaaagcgg acgacatctg gagtctgcgc
      301 aaggatgatt attttgttaa cgatgaagcg cgggcgcgtt actgggatga tcgtgaaaag
      361 gcccgtcttg cgcttgaagc cgcgagaaag aaggctgaac agcagagtca acaggacaaa
      421 aatgcgcagc agcagagcga tactgaagcg tcacggctga aatataccga agaggcgcag
      481 aaagcttacg aacggctgca gacgccgctg gagaaatata ccgcccgtca ggaagaactg
      541 aacaaggcac tgaaggacgg gaaaatcctg caggcagatt acaacacgct gatggcggcg
      601 gcgaaaaagg actatgaagc gacgctgaaa aagccatctg ctgtgaaggt gtctgccggt
      661 gagcgccagg aagaccgggc gcatgc
//
LOCUS       DAZFXT010000401          677 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.404, whole genome shotgun sequence.
ACCESSION   DAZFXT010000401 DAZFXT010000000
VERSION     DAZFXT010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.404"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..94)
                     /locus_tag="ACHKW0_005335"
     CDS             complement(<1..94)
                     /locus_tag="ACHKW0_005335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3950 domain-containing protein"
                     /protein_id="HFO7477949.1"
                     /translation="MSSKNRPRRTTTRNIRFPNQMIEQINIALDQ"
     gene            136..501
                     /locus_tag="ACHKW0_005336"
     CDS             136..501
                     /locus_tag="ACHKW0_005336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HFO7477950.1"
                     /translation="MAVLRTLPGRIKTLNTRRVNILKGEQRRVSGSARVSLKRHIWLR
                     DAGQCCLCGRVVDLCDSELDHRIALQFGGGNEETNLWTLCTECHRQKSAREAAGGMPD
                     PTLPEVSGGHGRADDIIGL"
BASE COUNT          140 a          155 c          201 g          181 t
ORIGIN      
        1 tctggtcaag agcgatgtta atttgttcaa tcatctggtt tggaaatcgg atgttgcggg
       61 ttgttgttct gcggggccgg tttttcgatg acattttctt tcctctggtg acaagctata
      121 tggcgaggat tttacatggc tgtgcttcgt acgttaccgg gcagaatcaa aactctgaac
      181 acccggcggg tgaatattct gaagggtgaa cagcgtcgtg tcagtggcag tgcacgtgtt
      241 tccctcaagc gtcatatctg gctcagggat gccgggcagt gctgtctctg tggtcgtgtg
      301 gttgacctct gtgacagtga actcgatcac cgaattgcac ttcagttcgg tggtggtaat
      361 gaggagacga atctctggac gctctgtacc gaatgccatc gacaaaagtc tgctcgtgaa
      421 gcggcgggtg gtatgccgga cccgacgctg ccggaggtgt ccggaggtca tggcagggca
      481 gacgatatca tcggactgtg acccgccccg ggggggggat catccggcga aaaaaacgat
      541 cgctccggac accgcgcccc ctctcacgca gagaaaaaat tcccgtttca gggcagttaa
      601 catgttaact ggctgcccgg gcatttttgc ggtttttatc tttattattc agtttgttgt
      661 gcggaaaaaa tgttaac
//
LOCUS       DAZFXT010000402          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.405, whole genome shotgun sequence.
ACCESSION   DAZFXT010000402 DAZFXT010000000
VERSION     DAZFXT010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.405"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..146)
                     /locus_tag="ACHKW0_005337"
     CDS             complement(<1..146)
                     /locus_tag="ACHKW0_005337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009645816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477951.1"
                     /translation="MYLHTYQHNDRLPPQKHDKGIIMEFSVIFFWVITGLITILFLKS
                     IITDL"
BASE COUNT          174 a          128 c          148 g          223 t
ORIGIN      
        1 agatcggtta ttattgattt taagaataag atggtaataa gaccagtgat aacccagaaa
       61 aaaataacgg aaaattccat aattatacct ttatcgtgtt tctggggtgg taatctatca
      121 ttgtgctgat atgtgtgtaa atacatgcat tatctgtttc ttatattctt tttatgaagt
      181 tactgattaa tttatattag aacatcacat tcataatgaa atcaacatgt tcatatggtt
      241 gggttgacgg cagatttccc tttttgttaa cctgtatttg cattaactgt cgcttttgcg
      301 accagccaat gttccagcag tctgagaggg acgccttcgg gtgactgcca gccaatgttt
      361 cagcaatctg agagaaacgc cttcgggtga ttgcaatatc ctcgtagcct gaaagaggat
      421 gcctttatgg tggtacatcc gtttagcctc tgggaacgga tatcactacg gtgattgagg
      481 tacgttgatt tttccttcgg attaccgtgg tgcttccgga caaccggact actgtgtatt
      541 cccggcagac tggcataaaa acgcctttaa aatctgcgtc ttaagatatt ttctgacttt
      601 ccgcatgcgc ttcttatgac tgtgcgcacg gcataagacc atttgaaatt tggtcgcttg
      661 cgtgtgcatg tgg
//
LOCUS       DAZFXT010000403          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.406, whole genome shotgun sequence.
ACCESSION   DAZFXT010000403 DAZFXT010000000
VERSION     DAZFXT010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.406"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            85..510
                     /locus_tag="ACHKW0_005338"
     CDS             85..510
                     /locus_tag="ACHKW0_005338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7477952.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGASVARIAREHDIND
                     NLLFKWLRLWQNEGRISRRLQVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TSVSAGSCKVEFRHGNMTLENPSPELLTLLIRELTGRGR"
     gene            507..581
                     /locus_tag="ACHKW0_005339"
                     /pseudo
     CDS             507..581
                     /locus_tag="ACHKW0_005339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309365.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          193 a          164 c          163 g          153 t
ORIGIN      
        1 taagcgtaaa ctgaccgccg tatgtagcca tcagacgaaa attggtaact tagacgccca
       61 tctgatatag acggacatct aagtatggaa ttacaggact ggcgaaaaga acctcgtaaa
      121 aactattcga atgaattcaa acttcgtatg gtggaactgg catcacaacc aggagcctct
      181 gttgcccgta ttgcccggga acacgatatc aatgataacc tcctgttcaa atggctcagg
      241 ctctggcaga acgaagggcg tatatcacgg cgtcttcagg taacgacctc ttctgacact
      301 ggcgttgaat tattacctgt agaaataacg ccggatgagc agaaagaacc ggtggcggcc
      361 attgcgccgt ctttatccac ttccactcag accagtgtca gtgccggctc ctgcaaggtg
      421 gagttccgtc acggtaacat gacgctggaa aatccttcac cagagctgct cacgctgttg
      481 atccgtgaac tgaccgggag gggacgatga tctcactccc gtcaggtacc cgcatctggc
      541 tcgttgccga cgttaccgat atgcgtaaat ccttcaacgg atacgacata tgctgaagga
      601 ggagcagtaa tggattaagt gataacaaat acccagaaat agaggttcaa atccagctta
      661 gttagtacat aaa
//
LOCUS       DAZFXT010000404          673 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.407, whole genome shotgun sequence.
ACCESSION   DAZFXT010000404 DAZFXT010000000
VERSION     DAZFXT010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..673
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.407"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>673
                     /locus_tag="ACHKW0_005340"
     CDS             <1..>673
                     /locus_tag="ACHKW0_005340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector protein NleH"
                     /protein_id="HFO7477953.1"
                     /translation="PDSRILSSVRDAVASSDNGAQVKVGNRTYRVVVTDNKFCVTRES
                     HSGCFTNLLHRLGWPKGEISRKIEVMLNSSPVNRTMERGAVHSNRPDLPPVDYAPPEL
                     PSVDYNRLPVPGNVIGKGGNAVVYEDAEDATKVLKMFTASQSNEEVTNEVRCFNQYYG
                     AGSAEKIYGDNGDIIGIRMDKINGESLLNISSLPAQAEHAIYDMFDRLEQKGILFIDT
                     TETNVL"
BASE COUNT          203 a          106 c          174 g          190 t
ORIGIN      
        1 tcctgacagt cgtattttat cctctgtaag ggatgcagtt gctagctcgg ataatggggc
       61 gcaagtaaag gtgggcaaca gaacatatcg tgttgttgtc actgataata agttttgcgt
      121 tacaagagaa agtcatagtg gttgctttac taatctgttg cacaggctgg gatggcctaa
      181 gggagagatt agcaggaaaa ttgaggtgat gctgaattca tcgccagtga ataggactat
      241 ggaaagaggt gctgttcatt cgaatagacc tgatttacct cctgttgatt atgcaccgcc
      301 agagttaccg agtgtggact ataacaggtt gcctgtgcct ggtaatgtta ttggcaaagg
      361 gggtaacgct gtagtatatg aagatgctga ggatgcaaca aaagtcctga agatgtttac
      421 tgcatctcaa agcaatgaag aggtaacaaa cgaagttcgt tgcttcaacc aatattatgg
      481 tgccgggagt gcagaaaaaa tatatggcga taatggcgat attattggta ttagaatgga
      541 taaaataaat ggagaatcgc ttttaaatat ttcgtccttg ccggcacagg ctgagcatgc
      601 catttatgat atgtttgaca ggcttgagca aaaaggaatt ctttttatcg atacaacaga
      661 gacaaatgtc tta
//
LOCUS       DAZFXT010000405          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.408, whole genome shotgun sequence.
ACCESSION   DAZFXT010000405 DAZFXT010000000
VERSION     DAZFXT010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.408"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..186
                     /locus_tag="ACHKW0_005341"
     CDS             <1..186
                     /locus_tag="ACHKW0_005341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO7477954.1"
                     /translation="QALRQARLDLLTGKRVVSVQKDGRRIEYTVASLDELNRAINDAE
                     SVLGTTRRRRRPLGVRL"
     gene            183..>667
                     /locus_tag="ACHKW0_005342"
     CDS             183..>667
                     /locus_tag="ACHKW0_005342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7477955.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSADA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEEKRTFTEFIREGVGVHAFNGEIFVQPVWDTESTQ
                     L"
BASE COUNT          138 a          148 c          234 g          147 t
ORIGIN      
        1 caggcgctac gtcaggcgcg ccttgattta ttaaccggta aacgggtggt gtctgtccag
       61 aaagatggtc gcagaattga atatacggtg gcttctctgg atgagcttaa ccgggcgatc
      121 aatgatgcgg agtcggtact ggggacaacc cgacgtcgcc gtcgtccgct gggagtgagg
      181 ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt tccgcttcgt gagagtctca
      241 gctacaacgg gggcggcgca ggatttggcg ggcaaatggc ggagtggttg ccaccggcgc
      301 agagtgccga tgcagccctg ctgcctgcgt tgcgtctggg gaatgcccgg gcagatgatc
      361 tggtgcgcaa taacgggata gcggccaatg cggtggccct gcataaggat catattgtcg
      421 ggcatatgtt tctgatcagc taccgtccga actggcgctg gctggggatg cgggagactg
      481 cggcaaaaag ttttgtcgat gaggtggagg cggcctggtc ggaatacgcc gaagggatgt
      541 ctggcgagat cgacgtggaa gagaaacgca cgtttacgga atttattcgt gaaggtgtgg
      601 gcgttcatgc gtttaacggc gaaatctttg tgcagccggt ctgggatacg gagagcacgc
      661 aactgtt
//
LOCUS       DAZFXT010000406          667 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.409, whole genome shotgun sequence.
ACCESSION   DAZFXT010000406 DAZFXT010000000
VERSION     DAZFXT010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..667
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.409"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..186
                     /locus_tag="ACHKW0_005343"
     CDS             <1..186
                     /locus_tag="ACHKW0_005343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family head-tail joining protein"
                     /protein_id="HFO7477956.1"
                     /translation="QALRQARLDLLTGKRVVSVQKDGRRIEYTAASLDELNRAINDAE
                     SVLGTTRRRRRPLGVRL"
     gene            183..>667
                     /locus_tag="ACHKW0_005344"
     CDS             183..>667
                     /locus_tag="ACHKW0_005344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7477957.1"
                     /translation="MKRTPVLIDVNGVPLRESLSYNGGGAGFGGQMAEWLPPAQSVDA
                     ALLPALRLGNARADDLVRNNGIAANAVALHKDHIVGHMFLISYRPNWRWLGMRETAAK
                     SFVDEVEAAWSEYAEGMSGEIDVEGKRTFTEFIREGVGVHAFNGEIFVQPVWDTETTQ
                     L"
BASE COUNT          140 a          152 c          232 g          143 t
ORIGIN      
        1 caggcgctgc gtcaggcgcg ccttgattta ttaaccggta aacgggtggt gtctgtccag
       61 aaagatggtc gcagaattga atatacggcg gcttctctgg atgagcttaa ccgggcgatc
      121 aatgatgcgg agtcggtact ggggacaacc cgacgtcgcc gtcgtccgct gggagtgagg
      181 ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt tccgcttcgt gagagtctca
      241 gctacaacgg gggcggcgca ggatttggcg ggcaaatggc ggagtggttg ccaccggcgc
      301 agagtgtcga tgcggccctg ctgcccgcgt tgcgtctggg gaatgcccgg gcagatgatc
      361 tggtgcgcaa taacggaata gcggctaatg cggtggccct gcataaggat cacattgtcg
      421 ggcatatgtt tctgatcagc taccgtccga actggcgctg gctggggatg cgggagaccg
      481 cagcaaaaag ctttgtcgat gaggtggagg cggcctggtc ggaatacgca gaagggatgt
      541 ctggcgagat cgacgtggaa ggaaaacgca cgttcacgga atttatccgt gaaggtgtgg
      601 gcgttcatgc gtttaacggc gaaatctttg tgcagccggt ctgggatacg gaaaccacgc
      661 agttatt
//
LOCUS       DAZFXT010000407          664 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.410, whole genome shotgun sequence.
ACCESSION   DAZFXT010000407 DAZFXT010000000
VERSION     DAZFXT010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.410"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..538
                     /locus_tag="ACHKW0_005345"
                     /pseudo
     CDS             1..538
                     /locus_tag="ACHKW0_005345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          256 a          118 c           96 g          194 t
ORIGIN      
        1 gttatatatt gagcacagtg aaaataaaaa caggatgaaa gtctttgccg gaaccaactt
       61 tattgatttc aatatgacag gacagaacct gtccgggttc gttctaacct tgtcaaggtt
      121 ttatttcgaa gatctactta atataaattt cactgacgca aatttaggtg acactatttt
      181 ttacataaag aacaccctac ccccaaatta tataaagatg gacaatatct tgacaaacaa
      241 atcgaaggtt tattttcaac attattaacc attaatgaca actttttacg agcaaaagcg
      301 gaaattgctt cgacaataat taaattttta gaggctagga taaccaatct ttcatacaat
      361 gacatattaa catatcagca agaattccaa aaacaatgtt ataagcaagt taaagccttt
      421 gcgacactct ctcgatacaa taaaattcaa acatgggcag aaatgtctga atatcagttc
      481 gaagtatttc aatacgagac gcttaacccc aaaaaaatgt cacacacgcc ttatttaaag
      541 aggccacttc ctaatgaaaa agatataaat tatggggtgg aaatagaaat accatctggc
      601 aaaagaattc gcttatcaaa tcattatcag aatataatac cataatattg attattagca
      661 cttt
//
LOCUS       DAZFXT010000408          663 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.411, whole genome shotgun sequence.
ACCESSION   DAZFXT010000408 DAZFXT010000000
VERSION     DAZFXT010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.411"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>663
                     /locus_tag="ACHKW0_005346"
     rRNA            <1..>663
                     /locus_tag="ACHKW0_005346"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          154 a          146 c          222 g          141 t
ORIGIN      
        1 tggtgggtag tttgactggg gcggtctcct cctaaagagt aacggaggag cacgaaggtt
       61 ggctaatcct ggtcggacat caggaggtta gtgcaatggc ataagccagc ttgactgcga
      121 gcgtgacggc gcgagcaggt gcgaaagcag gtcatagtga tccggtggtt ctgaatggaa
      181 gggccatcgc tcaacggata aaaggtactc cggggataac aggctgatac cgcccaagag
      241 ttcatatcga cggcggtgtt tggcacctcg atgtcggctc atcacatcct ggggctgaag
      301 taggtcccaa gggtatggct gttcgccatt taaagtggta cgcgagctgg gtttagaacg
      361 tcgtgagaca gttcggtccc tatctgccgt gggcgctgga gaactgaggg gggctgctcc
      421 tagtacgaga ggaccggagt ggacgcatca ctggtgttcg ggttgtcatg ccaatggcac
      481 tgcccggtag ctaaatgcgg aagagataag tgctgaaagc atctaagcac gaaacttgcc
      541 ccgagatgag ttctccctga ctccttgaga gtcctgaagg aacgttgaag acgacgacgt
      601 tgataggccg ggtgtgtaag cgcagcgatg cgttgagcta accggtacta atgaaccgtg
      661 agg
//
LOCUS       DAZFXT010000409          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.412, whole genome shotgun sequence.
ACCESSION   DAZFXT010000409 DAZFXT010000000
VERSION     DAZFXT010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.412"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..349)
                     /gene="wzx"
                     /locus_tag="ACHKW0_005347"
     CDS             complement(<1..349)
                     /gene="wzx"
                     /locus_tag="ACHKW0_005347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_099459345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O103 family O-antigen flippase"
                     /protein_id="HFO7477958.1"
                     /translation="MEKLNFAKVTILSGVVTCFRLICGLLISKVVAIYTGPIGIANLG
                     QLQNFVAFINGFISSQVSQGVNRYSAENQGDYENACFYWRASIKLSFIACCFIISTGV
                     LFSSKISEYLFYQS"
     gene            complement(337..>660)
                     /locus_tag="ACHKW0_005348"
     CDS             complement(337..>660)
                     /locus_tag="ACHKW0_005348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HFO7477959.1"
                     /translation="KYIEEIKNPLISLPVIAGNEMSHVWHLFVIRTSERDSLQKHLKT
                     EGITTLVHYPIAPHNQEAYKEYSFLSLPLTERIHREVLSLPMDPTMSIDDINMVIEAI
                     NRWKS"
BASE COUNT          221 a          117 c          101 g          221 t
ORIGIN      
        1 cactttgata aaataagtat tctgatattt tactggaaaa aagcaccccc gtacttataa
       61 taaaacaaca ggctatgaag gataatttaa ttgatgcacg ccaataaaaa catgcatttt
      121 cgtaatcacc ttgattttct gctgaatatc tattaactcc ttgtgaaact tgtgatgaaa
      181 tgaatccatt tataaaggcc acaaaatttt gcaattgtcc caaatttgct attccaattg
      241 gaccagtata aattgcaaca acttttgaaa taagaaggcc acaaattagt ctaaagcatg
      301 ttactacacc actgagtatc gtaacttttg cgaaatttag cttttccatc tattaatcgc
      361 ttcaattacc atattaatat cgtcgatgct cattgtcgga tccattggca gcgataagac
      421 ttctctatgt attctctcag ttaaagggag agataaaaaa ctatattctt tatatgcttc
      481 ttgattatga ggcgctattg ggtaatgcac taatgtagta ataccttcag ttttcaaatg
      541 tttttgcaaa gaatcacgct ctgaagttct gataacaaaa agatgccata catgagacat
      601 ttcattgcca gcaattacag gcagtgaaat tagtggattc ttgatttctt caatatattt
//
LOCUS       DAZFXT010000410          660 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.413, whole genome shotgun sequence.
ACCESSION   DAZFXT010000410 DAZFXT010000000
VERSION     DAZFXT010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 660)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 660)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..660
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.413"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..192
                     /locus_tag="ACHKW0_005349"
     CDS             <1..192
                     /locus_tag="ACHKW0_005349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7477960.1"
                     /translation="LSTYEKELALMGEDYQDIFRQQVRESAERQKAGLSRPVWIAQAY
                     QQQIAESRRPEEETTPRET"
     gene            182..>660
                     /locus_tag="ACHKW0_005350"
     CDS             182..>660
                     /locus_tag="ACHKW0_005350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7477961.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQFDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADSQVRGVLLDIDSPGGQAAGAFDCADMIYRLRQQKPVWALCNDTA
                     "
BASE COUNT          139 a          169 c          222 g          130 t
ORIGIN      
        1 ctgagcacgt atgagaaaga gctggcgctg atgggcgagg attatcagga cattttccgc
       61 cagcaggtca gggaatctgc agagcggcaa aaagccggac tctcacgtcc ggtgtggata
      121 gcgcaggcgt atcagcagca gatagcggag agtcgcaggc cggaagagga gacaacacca
      181 cgtgagacgt aatctttcac acattattgc cgcagcattc aatgaaccgc tgcttctgga
      241 gcccgcctat gcgcgggttt tcttttgcgc gctcgggcgc gagatggggg cagcaagtct
      301 ttcggtacca caacagcagg tacagtttga tgctcccgga atgctggctg aaacggacga
      361 gtacatggcc ggaggtaaac gaccggcccg tgtttacagg gtggtgaacg gtattgctgt
      421 actgccggtg accggcacgc tggtgcaccg gctggggggt atgcggccat tttccggaat
      481 gacaggctat gacggtattg tcgcctgtct tcagcaggca atggcggata gccaggtgcg
      541 gggcgtactg ctggacattg acagtccggg cgggcaggcc gccggcgcgt ttgactgcgc
      601 tgacatgatt taccgcctcc gtcagcagaa gccggtctgg gcactgtgca atgacacggc
//
LOCUS       DAZFXT010000411          658 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.414, whole genome shotgun sequence.
ACCESSION   DAZFXT010000411 DAZFXT010000000
VERSION     DAZFXT010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.414"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(89..271)
                     /locus_tag="ACHKW0_005351"
     CDS             complement(89..271)
                     /locus_tag="ACHKW0_005351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477962.1"
                     /translation="MYNCGESYSEYDISLSDDENYDDDYYRERPCPGEVFGYDNTEDN
                     DEAASDYALKINENSD"
BASE COUNT          194 a          148 c          107 g          209 t
ORIGIN      
        1 cgcccagatt aaaccaagta ttgagcgcct gattatgttt attcattaat atctgtcatt
       61 gattgtctta ttatcagcta cccccgtatt aatcagagtt ttcatttatt tttaatgcat
      121 aatcagatgc cgcctcatca ttatcctcag tattatcata gccgaaaact tctcccggac
      181 acgggcgttc acgataatag tcatcgtcat agttttcatc gtcagataat gatatgtcgt
      241 attctgaata tgattcacca caattataca tgaaatcatc tcctttgggg ggggcaataa
      301 ttagtgagtt tattcgaaaa aatgatgagc gccagaccaa tccacccaac ttttgattat
      361 aatcatgaag atacttgtct cagtacaaat tgaatcaaac tcaatgcagc atgattcacc
      421 tttatattct ctcagggaaa ccaaatacca ctactcaact attaagaaca aaattcggga
      481 tgtatgatta tcagaagata cctgcatcct aaataatttc agacagatat tatcgttgtg
      541 aatcccccgc cacctttccg gtgcgcgggg ttttgtcttt tttcaccggg aatacatgta
      601 tgaatccgtc tgatgccatt gaggcaattg aaaaaccgct ctcctccctg ccttactc
//
LOCUS       DAZFXT010000412          657 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.415, whole genome shotgun sequence.
ACCESSION   DAZFXT010000412 DAZFXT010000000
VERSION     DAZFXT010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..657
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.415"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..206)
                     /locus_tag="ACHKW0_005352"
     CDS             complement(<1..206)
                     /locus_tag="ACHKW0_005352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7477963.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFEL"
     gene            complement(206..535)
                     /locus_tag="ACHKW0_005353"
     CDS             complement(206..535)
                     /locus_tag="ACHKW0_005353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477964.1"
                     /translation="MKTFRWKVKPGMDVASVPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEATVLELFLEEHGGWKSFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
BASE COUNT          142 a          222 c          146 g          147 t
ORIGIN      
        1 ttcagttcaa aaccgctccc ctgaatggga tacggctgat actgtcgccc ctgccaggtg
       61 accggctcac ctttttcgtt ctgctcatta cagaaaaaat aacgttctcc accgacctct
      121 gtcaggtcga tttcccagag caccacgctg gccgactgct ccgcacgggt gcattcattc
      181 agtgtttcct gccggatatc ctgcatcagt tcaccacctg ttcaaactct gcgctgaact
      241 caacacgcag catactgacc cgcgacgacc attttgcgca ggtcaccttt atctgccgcc
      301 actcataagg cggcgtccac agaaaggatt tccagccccc gtgctcttcc agaaacaact
      361 ccagtaccgt ggcctcctca cgggggacag aaagcgtcac gctgtacgtt ttcaggttgg
      421 cattcagccc ggcaggcgct cgctgagaat agccatcacc aaagcgcacc tttcttacag
      481 aagggaccga agccacatcc ataccgggtt tcactttcca gcggaaggtc ttcatcgtcc
      541 acctccggag aacaggccac catcacgcat ctgtgtctga atttcatcac gggcaccctt
      601 gcgggccatg tcatacaccg ccttcagagc agccggacct atctgcccgt tcgtgcc
//
LOCUS       DAZFXT010000413          655 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.416, whole genome shotgun sequence.
ACCESSION   DAZFXT010000413 DAZFXT010000000
VERSION     DAZFXT010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.416"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..431
                     /locus_tag="ACHKW0_005354"
     CDS             <1..431
                     /locus_tag="ACHKW0_005354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HFO7477965.1"
                     /translation="VGCGALWLATNHYRDNALTYKAQRDKKARELEQANATITDMQVR
                     QRDVAALDAKYSRELADARAENETLRADVAAGRKRLRINAICSGTVREATGTSGVDNA
                     TGPRLADTAERDYFILRERLMTMQKQLEGAQDYIRTQCLN"
BASE COUNT          161 a          171 c          188 g          135 t
ORIGIN      
        1 cggttggctg tggtgcgctg tggctggcaa caaaccatta ccgtgacaac gcgctcacct
       61 acaaagcgca gcgtgataaa aaagccagag agctggaaca ggcgaatgcc accattactg
      121 acatgcaggt gcgccagcgt gatgttgctg cgctcgatgc aaaatactcg agggagttag
      181 ccgatgcgag agctgaaaat gaaactctgc gtgctgatgt tgccgctggt cgtaagcgcc
      241 tgcggatcaa cgccatctgc tccggtaccg tgcgtgaagc caccggcacc tccggcgtgg
      301 ataatgcaac cggcccccga ctggcagaca ccgctgaacg ggattatttc atcctcagag
      361 aacggttgat gacaatgcag aagcagctgg aaggggcaca ggactatatc cgcactcagt
      421 gcctgaacta agttttgctg atgcgccgta tcgtcaccgt attcctgcat taacagagac
      481 cgcagcccga cagggagact cctctgcgag agtgtgcggg gataatcaaa aacgatacac
      541 accggggttt accgcgttaa cggagcgcgg tgttgtcccc tcatagtcgc cagtccggtg
      601 cgatggtgga agaaactgga ttttgttgca aatgataacc attatcattt ttgcg
//
LOCUS       DAZFXT010000414          653 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.417, whole genome shotgun sequence.
ACCESSION   DAZFXT010000414 DAZFXT010000000
VERSION     DAZFXT010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.417"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>653)
                     /locus_tag="ACHKW0_005355"
     CDS             complement(<1..>653)
                     /locus_tag="ACHKW0_005355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HFO7477966.1"
                     /translation="PGLNRDNFIYISNIFLNIKQRNEKNHTINMFREVSISNDTISVK
                     FYRNEKIECACNFLMDKDAQGYIDLSDMNLTSCHFKGDVISKVSFLSSNLQHVTFECK
                     EIGYCNFTTATVDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSRSQLTHSNFR
                     ECQIRNSNFDHCYLYASHFTRAEFLSDKEISFIKSNLTAVMFDHVRISTGNFKDCIT"
BASE COUNT          225 a          113 c           90 g          225 t
ORIGIN      
        1 tgtaatgcaa tctttaaaat tccctgtcga tattcgcaca tgatcaaaca taacagctgt
       61 caaattcgat ttgataaatg atatttcttt gtcagaaaga aattctgctc tggtgaagtg
      121 cgaagcataa agataacaat gatcgaagtt tgaatttcta atctgacatt cgcgaaaatt
      181 actatgagta agctggctcc gcgagaagtc aactgtatca agaatatttt tgctaaaatc
      241 gacacattca ccactcgctt tgataaaaat cacattgtgt aaacgtcgac atctaaatat
      301 aacattatca actgttgcag tagtaaaatt gcaataccca atttctttac attcgaatgt
      361 tacatgttgt agatttgatg ataaaaaaga caccttcgat ataacgtcac ctttaaaatg
      421 acaacttgtt aaattcatat cagacaggtc gatatacccc tgcgcatctt tatccatcag
      481 aaaattacaa gcgcattcta ttttttcatt tctgtagaat tttacactta tagtatcatt
      541 actaattgag acttcacgaa acatatttat tgtatgattt ttttcgttcc gttgttttat
      601 atttaaaaaa atattgctga tataaataaa attatctcta tttaaacctg gca
//
LOCUS       DAZFXT010000415          642 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.418, whole genome shotgun sequence.
ACCESSION   DAZFXT010000415 DAZFXT010000000
VERSION     DAZFXT010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.418"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..462)
                     /locus_tag="ACHKW0_005356"
     CDS             complement(<1..462)
                     /locus_tag="ACHKW0_005356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase
                     domain-containing protein"
                     /protein_id="HFO7477967.1"
                     /translation="MIKKISINFLFLMLMIDVVFATLFNIPVWMHLFNIINNLDGVKL
                     GFIISLPVFLISALNFVFTPFSFRYIFKPFFCILFICSSIVTYATMKYGVQFDKTMMQ
                     NIFETNAGEMTSYFNMSVVLWFLFTGILPCGLLLLVNIRYPETWIKGIIYRL"
BASE COUNT          259 a          109 c           81 g          193 t
ORIGIN      
        1 taaacgatat ataataccct ttatccatgt ctcaggataa cggatattta ctaacaacaa
       61 tagtccacat ggaagtatac ccgtaaataa aaaccacagt acaacagaca tattgaaata
      121 tgatgtcatt tcacctgcat ttgtttcaaa aatattctgc atcattgttt tatcaaactg
      181 cacaccatat ttcatggtgg catatgtaac aatggaacta cagataaaca gaatacaaaa
      241 aaaaggttta aatatatacc tgaatgagaa cggtgtaaaa acaaaattta atgcagatat
      301 cagaaagact ggaagagata taataaatcc aagtttcaca ccatcaaggt tattaataat
      361 attaaacaga tgcatccaga cagggatatt gaacaatgtg gcaaaaacaa catctatcat
      421 taacatcaag aaaagaaaat taatactgat ttttttaatc atcttacccc cattaattag
      481 agtctataat tgtccgtatc taaaagtgat atatttttat aaaacctggt aaagtgaata
      541 ctgatttata ccaggtcaaa cttcaacttc atcatcaaaa ctttatgtca ataaaggcgt
      601 ttatagttac tgattctttt attttacaac accactaaca tc
//
LOCUS       DAZFXT010000416          641 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.419, whole genome shotgun sequence.
ACCESSION   DAZFXT010000416 DAZFXT010000000
VERSION     DAZFXT010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..641
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.419"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            38..>641
                     /locus_tag="ACHKW0_005357"
     CDS             38..>641
                     /locus_tag="ACHKW0_005357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HFO7477968.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDKDTDQ
                     EDQNTMVHGVKNATNQAGDVQTVNPGQPAGTTPEADSAYAL"
BASE COUNT          206 a          137 c          182 g          116 t
ORIGIN      
        1 tcgtgagtaa aaagattcgg tatcagaaga ggtgagtatg gctaacgcct ggctcagatt
       61 atggcatgac atgccaaatg accctaagtg gcgaacaatt gccagggtgt cagggcagcc
      121 aattgcaaca gtgatggcag tgtatatcca cctcctggtg agcgcgtcac gaaatgtcac
      181 gcgaggtcac attgatgtca cgacagaaga tttggcaagt gcgctcgacg tgacagaaga
      241 ggtaattgat tcaattttgc agacgatgca ggggcgggta cttgatggtg atttaatcac
      301 tggatgggaa aaacgccagg tgcttaaaga ggacaacggc aatatttcgc aaaccgcaaa
      361 atctcctgca gagcgcaaga gggcgcagcg agagagggaa agaaagcggg aacaaaatgg
      421 cgattgtcac ggcgcgtcac gaaatgtcac gcacatgtca cgacgagtca cgacagataa
      481 agatacagat aaagatacag ataaagatac agatcaagaa gatcaaaaca ctatggtcca
      541 tggcgtaaaa aacgccacga accaggcagg ggatgttcag accgtcaatc ctggtcagcc
      601 agcaggcacg acaccggaag ccgattcagc gtatgcgctg a
//
LOCUS       DAZFXT010000417          638 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.420, whole genome shotgun sequence.
ACCESSION   DAZFXT010000417 DAZFXT010000000
VERSION     DAZFXT010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.420"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>638
                     /locus_tag="ACHKW0_005358"
     CDS             <1..>638
                     /locus_tag="ACHKW0_005358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HFO7477969.1"
                     /translation="DFLGAMSEDDVRPEALRRFELMVEEAARHAEEAKKNAGEAETSA
                     RNAGISASQAEESAANADTSAGEASESARQAAESAASAKQSEEASSSSASAAAQKASE
                     SSQSAAEAELSRKTAESAAGNAARDATTAAEKARESAESAQSAEQSRIAAEEAVNRIP
                     TVVGPPGPKGEPGPAGPQGPKGDKGERGDTGPAGATGERGPAGDAGPAGPQG"
BASE COUNT          164 a          162 c          237 g           75 t
ORIGIN      
        1 atgattttct cggtgccatg tcggaggatg acgtccggcc ggaggcactg cgtcgttttg
       61 aactgatggt ggaagaagcg gcgcgtcacg ctgaggaggc gaagaagaat gccggagagg
      121 cggagacgtc cgcgaggaat gccggcatat cagccagtca ggcagaagag agcgcggcaa
      181 atgctgacac ttcagcaggg gaggcatcgg agtcagcccg gcaggcggca gaaagtgcag
      241 cctcagcaaa gcagtcagag gaggcgtcct cgtcctcggc ttctgcggcc gctcaaaaag
      301 ccagtgagtc atcacaaagt gcagcagaag ctgaattgtc aagaaagacg gcagaaagtg
      361 cagccggtaa tgcagccagg gatgcaacga ccgcagcaga aaaagcccgg gagtcagcag
      421 aaagcgcaca gtcagcggaa caaagcagga tagcggcgga agaagccgta aaccgaatcc
      481 cgaccgtggt ggggcctccc gggccaaagg gggaaccggg tcccgcgggt cctcaggggc
      541 cgaagggaga taaaggagag cgtggcgaca ccggcccggc aggggcaacc ggtgaaaggg
      601 ggccggcagg tgatgctggt ccggcaggcc cgcagggg
//
LOCUS       DAZFXT010000418          636 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.421, whole genome shotgun sequence.
ACCESSION   DAZFXT010000418 DAZFXT010000000
VERSION     DAZFXT010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.421"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>636
                     /locus_tag="ACHKW0_005359"
     CDS             <1..>636
                     /locus_tag="ACHKW0_005359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_305459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7477970.1"
                     /translation="RQFRNVTAEQIAYVAQLQRSGDEAGALQAANDAATKGFDEQTRR
                     LKENMGTLETWADKTGKAFKSMWDAILDIGRPESSADMLASAQKAFDEADKKWQWYQS
                     RSQRRGKTSSFRANLQGAWDDRENARLGLAAATLQSDMEKAGELATRDRAERDASQLK
                     YTGEAQKAYERLLTPLEKYTNRQEELNKALKDGKILQADYNTLMAAAKKDYE"
BASE COUNT          165 a          152 c          217 g          102 t
ORIGIN      
        1 cgccagttcc gtaacgtgac ggcagagcag attgcgtatg ttgcgcagct gcagcgttcc
       61 ggtgatgagg ccggggcctt acaggcggcg aacgatgctg ccacgaaagg ctttgatgag
      121 cagacccgtc gcctgaaaga aaacatgggg acgctggaga cctgggcgga taaaacaggg
      181 aaggcgttca aatcgatgtg ggatgccatt ctggatattg gtcgtccgga atcctcagcg
      241 gatatgcttg ccagtgcgca gaaggcattc gatgaggcgg ataaaaaatg gcagtggtac
      301 cagagccgga gccagcgccg gggaaagacc tcctctttcc gtgccaacct gcagggcgca
      361 tgggatgacc gggaaaatgc ccgtctgggg ctggcagcgg ccacgctgca gtcggatatg
      421 gaaaaagccg gtgaactggc taccagggac cgggccgaac gggacgcatc acagctgaag
      481 tataccggag aggcgcagaa ggcgtatgag cgtctgctga cgccgctgga gaaatatact
      541 aaccggcagg aagagctgaa taaggccctg aaagacggga aaatcctgca ggcggattac
      601 aacacgctga tggcggcggc gaaaaaggat tatgaa
//
LOCUS       DAZFXT010000419          632 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.422, whole genome shotgun sequence.
ACCESSION   DAZFXT010000419 DAZFXT010000000
VERSION     DAZFXT010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..632
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.422"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            361..>632
                     /locus_tag="ACHKW0_005360"
     CDS             361..>632
                     /locus_tag="ACHKW0_005360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2528 family protein"
                     /protein_id="HFO7477971.1"
                     /translation="MSNIKKYIIDYDWKASIEIEIDHDVMTEEKLHQINNFWSDSEYR
                     LNKHGSVLNAVLIMLAQHALLIAISSDLNAYGVVCEFDWNDGNGQEG"
BASE COUNT          214 a          120 c          131 g          167 t
ORIGIN      
        1 acaagaactt tctggtacaa tagatgtcat cttaggttct aggtttataa aagaactgaa
       61 agaaagcagg tctttctctc gtgtagagat aagcgagtca gagttatggg gtatatcaaa
      121 taatcctgta aaccacgctc accaacgaaa cccaaacaaa aagtggtcat aacccgctca
      181 ggcggatttc attttcacgc aaacaacaga ataaacacag cgctgtgtat tcattccaac
      241 gagtgaatac acggagcaat gtcgctcgta actaaacagg agccgacttg ttctgattat
      301 tggaaatctt ctttgccctc caagatgagg gcgatttttt atctgtgagg atatgaacag
      361 atgtcaaaca tcaaaaaata catcattgat tacgactgga aagcatcaat agaaattgaa
      421 atcgaccatg acgtaatgac agaggaaaaa cttcaccaga ttaataattt ctggtcagac
      481 tctgaatacc gactcaataa acacggctct gtattaaatg ctgtattaat catgctggcg
      541 caacatgctc tgcttatagc aatttcaagc gacttaaatg catatggtgt tgtgtgtgag
      601 ttcgactgga atgatggaaa tggtcaggaa gg
//
LOCUS       DAZFXT010000420          619 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.423, whole genome shotgun sequence.
ACCESSION   DAZFXT010000420 DAZFXT010000000
VERSION     DAZFXT010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..619
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.423"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>619
                     /locus_tag="ACHKW0_005361"
     CDS             <1..>619
                     /locus_tag="ACHKW0_005361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HFO7477972.1"
                     /translation="TPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEIRYR
                     YDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEHADG
                     SVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQLTS
                     ATGPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCAT"
BASE COUNT          155 a          178 c          193 g           93 t
ORIGIN      
        1 ggacgccacg cggcgaactg gcggtggtgt atgaccgtag tggcaaacag gtgcgcagct
       61 ttacttacga tgataaatac cggggccgga tggtggcgca ccgtcacacg ggccggccgg
      121 aaatccgtta ccgttacgac agcgacgggc gggtgacaga acagcttaac ccggcaggct
      181 taagctacac gtatcagtat gagaaagacc acatcaccat caccgacagc ctggaccgcc
      241 gtgaagtgct gcacacgcag ggcgaagccg ggctgaagcg ggtggtgaaa aaggaacacg
      301 cggacggcag cgtcacgcag agtcagtttg atgcggtggg caggcttaag gcacagacgg
      361 atgccgcagg ccggacaaca gagtacagcc cggatgtggt gacgggcctc atcacgcgca
      421 tcaccacgcc ggatggcagg gcatcggcgt tttactataa ccaccacagc cagttaacgt
      481 cagccaccgg gccagacggg ctggaaatgc gccgcaaata tgatgagtac ggacgtctga
      541 ttcaggaaac cgcccctgac ggcgatatca cccgctaccg ttatgataat ccacacagtg
      601 acctcccctg cgcaacgga
//
LOCUS       DAZFXT010000421          615 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.424, whole genome shotgun sequence.
ACCESSION   DAZFXT010000421 DAZFXT010000000
VERSION     DAZFXT010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..615
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.424"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>615
                     /locus_tag="ACHKW0_005362"
     CDS             <1..>615
                     /locus_tag="ACHKW0_005362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477973.1"
                     /translation="STLKGALDINSLIIDYDNEDVQSYWEDVVLPNLNDNGNKALTRD
                     ETIAYIKDSKPCRRIKRLIHDDAVLEDQKDKNFIAKMIAEYAVSRNAIISSNTSGNVI
                     RGDESGVKWIQCGNIFVSLFHKVQDDHENDGDRIWQTLNDSLIEWKPSYYQLIKSEIQ
                     NAIEAEALSFVNHLANDHYGQAAWLNEILKSDSPDIRCRNIDFV"
BASE COUNT          217 a           90 c          123 g          185 t
ORIGIN      
        1 catcgactct caaaggtgct ctggatatca acagcttgat cattgactac gataatgaag
       61 atgtccaaag ttattgggaa gacgttgtat taccgaactt aaatgataat ggtaataaag
      121 ctctcacaag agatgaaaca atagcctata ttaaagacag taagccttgt agaagaatta
      181 aaagattaat acatgatgat gctgtgttgg aggatcaaaa ggataaaaac ttcattgcaa
      241 aaatgattgc agaatatgct gtgtctagaa atgcaattat ttctagcaac acatctggca
      301 atgtcattcg gggtgatgaa agcggagtaa aatggattca atgtggtaat atctttgtct
      361 ccctatttca taaggttcaa gatgatcatg aaaacgatgg agataggatt tggcaaactc
      421 tcaatgattc tctcattgaa tggaaaccat cttattatca gttaataaaa tctgaaattc
      481 agaatgcaat cgaagctgag gctttatctt ttgtaaatca tttggctaac gatcattacg
      541 gtcaagctgc gtggttaaat gagatattaa aatcagactc gcctgatatt agatgtagaa
      601 atattgactt tgtat
//
LOCUS       DAZFXT010000422          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.425, whole genome shotgun sequence.
ACCESSION   DAZFXT010000422 DAZFXT010000000
VERSION     DAZFXT010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.425"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>608
                     /locus_tag="ACHKW0_005363"
     CDS             <1..>608
                     /locus_tag="ACHKW0_005363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7477974.1"
                     /translation="KNKTEKNIHFVPFMTDENGVNVPTNKPEEDPDIPGIGYYGSKWR
                     DSSATWTSQDRASHFSAWARRGIISDRLATAILRHAGRVALNAGASSTVSEVRPSSPS
                     GAEATGVTTLLSYLASESEGSLKVQGWSASGGRAEVVSDAEGTGGKAVKLTKEAGKSS
                     WVLEYAAGNGAALLQKGGQIRCRFKVSGALAANQYVMAFYWPV"
BASE COUNT          148 a          148 c          203 g          109 t
ORIGIN      
        1 aaaaacaaaa cggaaaagaa tatccatttc gtaccgttca tgaccgatga gaacggggtg
       61 aatgtgccga cgaacaaacc ggaagaagac ccggacattc cgggtatcgg atattacggt
      121 tcgaaatggc gtgacagctc agccacctgg acgtcacagg acagggcgag ccatttcagc
      181 gcctgggcac gccgtgggat tatttccgac cgtctggcaa cggcgatttt gcgccatgcg
      241 ggaagagtgg cgctaaacgc gggggcatca tcgacagtat cagaggtgcg cccttcatcg
      301 ccttccggtg cagaagccac aggcgtcaca acactgctct cttaccttgc cagcgagtca
      361 gagggaagcc tgaaagtaca gggatggtca gccagtggcg gcagggcaga agtggtcagc
      421 gatgcggagg gaaccggagg taaggcagtg aagctgacca aggaggccgg taaaagcagc
      481 tgggtgctgg agtacgccgc gggcaacggt gcggctctgt tacagaaagg ggggcagatt
      541 cgctgccgct ttaaggtttc gggagcgctg gctgcgaacc agtatgttat ggcgttttac
      601 tggccggt
//
LOCUS       DAZFXT010000423          608 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.426, whole genome shotgun sequence.
ACCESSION   DAZFXT010000423 DAZFXT010000000
VERSION     DAZFXT010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..608
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.426"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..608
                     /locus_tag="ACHKW0_005364"
                     /pseudo
     CDS             1..608
                     /locus_tag="ACHKW0_005364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308875.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="Non-LEE-encoded type III effector H"
BASE COUNT          188 a           98 c          155 g          167 t
ORIGIN      
        1 gcctgataat cgtgttttat ccctctgtaa gggatgctgc cgctcactct gataatgggg
       61 cgcaagtaaa ggttagcaac agaacatatc gcgttgtttt aagtgataat aggttttgcg
      121 tgacaagaga gagtcatagt ggatgtttta ctaatatgct gtatagactg ggatggccta
      181 agggagagat caccagaaaa attgaggcta tgctgaattc atcgacagtg agcacgacta
      241 tagaaagagg atctgttcgt tcgaacagac ctgatttacc tccagtggat tatgcgcagc
      301 cggagttacc gccagcggat tatactcagt cagcgttgct gaggcttagc aacaacaaat
      361 cacccgtgct aggtaacgtt attggtaaag gtggtaatgc tgtcgtgtat gaagatatgg
      421 aaaatacaac aaaagtgttg aagatgttta ctatatctca aagccatgaa gaggtgacaa
      481 gcgaagttcg ttgtttcaac cagtattatg gtgccgggag tgcagagaaa atatatggcg
      541 ataatagaga tattattggt attagaatgg ataaaataaa tggagaatcg cttttaaata
      601 tttcgtcc
//
LOCUS       DAZFXT010000424          597 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.427, whole genome shotgun sequence.
ACCESSION   DAZFXT010000424 DAZFXT010000000
VERSION     DAZFXT010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..597
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.427"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..585
                     /gene="espW"
                     /locus_tag="ACHKW0_005365"
     CDS             <1..585
                     /gene="espW"
                     /locus_tag="ACHKW0_005365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector EspW"
                     /protein_id="HFO7477975.1"
                     /translation="QIADEVKSLTYLIPSEKKEMWIYKNNGKDNAKPNLGERDVRMFE
                     NISPDDTDKITGRKFSELGEYLYSGNVIKLSQLSIRYLPNINSISLIETKQSLLLHRL
                     YSDEVLQRNGTLIPTPLHEEKSIPADNIKTMLNNIPTYKMLPPFTETQGNCSSGAATF
                     LRKSGAEEKDILACSPRNYGLHHNIKTWDPLVRN"
BASE COUNT          238 a          108 c          101 g          150 t
ORIGIN      
        1 caaattgcag atgaagtaaa atcacttaca tatttgattc cttcagaaaa aaaagagatg
       61 tggatatata aaaacaatgg aaaagataat gcaaaaccaa acttaggaga gcgagacgta
      121 agaatgttcg aaaatattag tcctgatgat acagataaga taacaggaag gaaattttca
      181 gagttaggtg agtatcttta ttcaggaaat gtaataaaac tcagtcagtt atcaattcgt
      241 tacttaccca atatcaactc aatctcatta atagagacaa aacagagttt gttgctacat
      301 cgattatatt cagatgaagt acttcagaga aatggaacac ttatcccgac accactacat
      361 gaagaaaaat caattccagc tgacaatata aaaacaatgc tcaacaacat accgacttac
      421 aaaatgttac cgccattcac agaaacacaa ggtaattgtt cttctggcgc agccacgttt
      481 ttacgcaaat caggcgccga agaaaaagat attcttgcat gtagcccccg aaattatggg
      541 ctgcatcata acataaaaac atgggacccc ttggttagaa attaaggatc caaatat
//
LOCUS       DAZFXT010000425          596 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.428, whole genome shotgun sequence.
ACCESSION   DAZFXT010000425 DAZFXT010000000
VERSION     DAZFXT010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.428"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>596
                     /locus_tag="ACHKW0_005366"
     CDS             <1..>596
                     /locus_tag="ACHKW0_005366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7477976.1"
                     /translation="RLGYAADDLGFVGDPYQEARQRYISNALEAWRNNEANKPKSRGG
                     KSETEKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTALGELKTLSEIQKQELLRNA
                     ALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMRELQQIRDSFR
                     QKDADLQSQYQTGDISEDFYRQALAQNAQYLSERLKEQ"
BASE COUNT          168 a          136 c          195 g           97 t
ORIGIN      
        1 ccggctgggg tatgcggcgg atgacctcgg ttttgtgggt gatccgtatc aggaggcgag
       61 acaacgttat atcagtaatg ccctggaagc ctggcgcaat aacgaggcga ataaacccaa
      121 atcccggggt ggaaaatcag agacggaaaa agcggaagac agtttttccc ggctgctgaa
      181 gcagcagaaa gagcaactgg cactggccgg tcagaacacg gagctggcga agctgaagta
      241 ccagacagcg ctgggtgaac tgaaaaccct gtcggagata cagaagcagg agctgctgcg
      301 taacgcggcc ctgattgacc agcaaaaaat ccgggaacag ttgcgatccc gggaagagac
      361 cctgaagaat gataatgtgg ctgcgcgtgc atcaaatgaa gccgaactgc tggggtacgg
      421 gcagggagaa cgagccaggg aacgcatgcg ggagttgcag cagatccgcg acagcttccg
      481 ccagaaggat gcggaccttc agtctcagta tcagaccggg gatatcagtg aggattttta
      541 cagacaggcg ctggcgcaga atgcacagta tctgagtgaa cgtctgaaag agcagg
//
LOCUS       DAZFXT010000426          594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.429, whole genome shotgun sequence.
ACCESSION   DAZFXT010000426 DAZFXT010000000
VERSION     DAZFXT010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.429"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>594
                     /locus_tag="ACHKW0_005367"
     CDS             <1..>594
                     /locus_tag="ACHKW0_005367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477977.1"
                     /translation="RAAGGAGALSDWLLRHVKSCQWLHGDYHHSETVIHRYGTGAMVL
                     CWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQV
                     ADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQRTKNLAPLPHAHQQQN
                     PPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWV"
BASE COUNT          145 a          166 c          162 g          121 t
ORIGIN      
        1 ccgtgctgcg ggtggcgctg gtgcactgtc tgactggtta ttacgtcacg tgaaatcctg
       61 ccagtggcta cacggtgatt atcatcacag cgaaaccgtc attcaccgtt acggtaccgg
      121 cgcaatggtg ttgtgctggc actgcgacaa ccagctgcgt gaccagacat ccgaatcact
      181 cgagcaactt gctcatcaaa acctgtcagc atggatgatt gacgtcatcg gtcacgcaat
      241 aagcggtacg caggagcgtg aattatctct ggctgaatta tcctggtggg cggtccgcaa
      301 tcaggtggcg gacgcgctac cggaagcggt attacgtcgt tcgctggggt tgcgtgcgga
      361 aaaaatccgc tccatgtacc gtgaaagcga catcgtaccg ggagagcaga ccgccaccag
      421 catactgaaa cagcgcacaa aaaatcttgc gccgctgcct cacgcccacc agcaacagaa
      481 cccaccacag gaaaagacgg tggtcagcat tgccgttgat cctgagtctc cggaatcttt
      541 catgaaacga cctaaacgtc gccgctgggt taacgagaaa tacactcgct gggt
//
LOCUS       DAZFXT010000427          594 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.430, whole genome shotgun sequence.
ACCESSION   DAZFXT010000427 DAZFXT010000000
VERSION     DAZFXT010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 594)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 594)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..594
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.430"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>594
                     /locus_tag="ACHKW0_005368"
     CDS             <1..>594
                     /locus_tag="ACHKW0_005368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7477978.1"
                     /translation="RAAGGAGALSDWLLRHVKSCQWLHGDYHHSETVIHRYGTGAMVL
                     CWHCDNQLREQTSDSLDQLAQQNLAAWMIDIIRHAMNGAQERELSLAELSWWAVRNQV
                     ADALPEAVLRRSLGLRAEKIRSVYRESDIIPGEQTATSILKQRTKNIALPSHTHQQQN
                     PPQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWV"
BASE COUNT          148 a          163 c          163 g          120 t
ORIGIN      
        1 ccgtgctgcg ggtggcgctg gtgcactgtc tgactggtta ttacgtcacg tgaaatcctg
       61 ccagtggcta cacggtgatt atcatcacag cgaaaccgtc attcaccgtt acggtaccgg
      121 cgcgatggtg ttgtgctggc actgcgacaa ccagctgcgg gagcagacat ctgattcact
      181 ggatcaactt gctcaacaga atctggccgc ctggatgatt gacatcatcc gtcacgcaat
      241 gaatggcgca caggagcgtg aattatctct ggctgaatta tcctggtggg cggtccgcaa
      301 tcaggtggcg gacgcgctac cggaagcggt attacgtcgt tcgctggggt tgcgtgcgga
      361 aaaaatccgc tccgtatacc gtgaaagcga catcataccg ggagaacaga ccgccaccag
      421 catactgaag cagcgcacaa aaaatattgc gctaccgtct cacacccacc agcaacagaa
      481 cccaccacag gaaaagacgg tggtcagcat tgccgttgat ccggagtctc cggaatcctt
      541 catgaaacga cctaaacgtc gccgctgggt aaatgagaaa tacacacgct gggt
//
LOCUS       DAZFXT010000428          578 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.431, whole genome shotgun sequence.
ACCESSION   DAZFXT010000428 DAZFXT010000000
VERSION     DAZFXT010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..578
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.431"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>578
                     /locus_tag="ACHKW0_005369"
     CDS             <1..>578
                     /locus_tag="ACHKW0_005369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="9-O-acetyl-N-acetylneuraminic acid deacetylase"
                     /protein_id="HFO7477979.1"
                     /translation="TNAPAEDPDIPASGYYGAASRTNGNQVSSNRPTHFSSWARRSII
                     PDRLATAILNAAGRTSAFISGKAPEIKPSPGGNTPSGPSADTSVRTISLLPAAGEAAA
                     QGWSIKDGGIQLSDGVFKITKQSNKTWSLTHPVDDAITLLTQGGRLTCKFRLSGALTN
                     NQFGLGIYLYTDAPVPDGVAMTGTGNPFLMSY"
BASE COUNT          133 a          162 c          163 g          120 t
ORIGIN      
        1 actaacgcgc cggcagaaga tccggatatt ccggcatcag gatattacgg tgcggcatcg
       61 agaacgaatg gaaaccaggt atcatcaaac cgcccgacac atttcagttc atgggcgcgc
      121 aggagcatta ttccggatcg tctggcaacc gctattctga acgcagccgg gcgcacctca
      181 gccttcatca gtggtaaggc accggaaatc aaaccctcgc ccggcggcaa cacgccatcg
      241 ggtccgtctg cagatacgtc cgttcgcaca atctccctgc tgccggcagc cggagaggct
      301 gctgcgcagg gctggagcat taaggatggc ggaattcagt tgtcagatgg tgtatttaag
      361 atcaccaagc agagcaataa aacctggtcc ctgacgcatc cggtggatga cgcaattacc
      421 ctgctgacac agggcggcag actgacctgt aagttccgcc tgtcaggcgc actgaccaac
      481 aatcagttcg ggctggggat ttatctgtat acggatgctc ccgttcctga tggtgtggcg
      541 atgacgggta ccggtaatcc gttcctgatg tcgtactt
//
LOCUS       DAZFXT010000429          571 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.432, whole genome shotgun sequence.
ACCESSION   DAZFXT010000429 DAZFXT010000000
VERSION     DAZFXT010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..571
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.432"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(479..>571)
                     /locus_tag="ACHKW0_005370"
     CDS             complement(479..>571)
                     /locus_tag="ACHKW0_005370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477980.1"
                     /translation="KVYGCEYITRRVKGLTQTNVTTTEDCDAFL"
BASE COUNT          114 a          133 c          115 g          209 t
ORIGIN      
        1 tcgggattct ccttcgcgta tccttcccgt gtgtggcggt ctataacttt gattgccacc
       61 tttagggaaa gctctgtcat gtctaacgct ttatattttt gctgtgttct gtctctgcgc
      121 attttggtca tttgtcgccc ctgattcatg ttttcggtcg gcatgtttgt taagtgattt
      181 tgatgtatgc acatttattt ttaccccctc gtttaaaaag ttttaggttg tggtgcacct
      241 cctctacctc tctacctaat catctttcag gtcagtaatg gcgcggcttt cagatgggta
      301 gagggctttc gtggccctct acccatcatc tacccgcccc tcacaaacaa ccttaatcat
      361 ggtggagggg gtagagggct tttatttgcc ttctaccagc ccctctaccc acttatcata
      421 ttgaataatg tatatttatt ttctttaggt agaagaggta gagggctttt gtctaaaatt
      481 ataaaaacgc gtcgcaatcc tctgttgttg tcacattggt ttgcgtaagt cctttaaccc
      541 tgcgcgttat atattcacat ccgtaaactt t
//
LOCUS       DAZFXT010000430          567 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.433, whole genome shotgun sequence.
ACCESSION   DAZFXT010000430 DAZFXT010000000
VERSION     DAZFXT010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.433"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..422)
                     /locus_tag="ACHKW0_005371"
     CDS             complement(<1..422)
                     /locus_tag="ACHKW0_005371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7477981.1"
                     /translation="MGKGGGKAHTPVEAKDNLKSTQMMSVIDAIGEGPIEGPVKGLQS
                     ILVNKTPLTDTDGNPVIHGVTAVWRAGEQEQTPPEGFESSGAETALGVEVTKAKPVTR
                     TITSANIDRLRVTFGVQSLVQTTSKGDRNPTSVRLLIQ"
BASE COUNT          110 a          175 c          138 g          144 t
ORIGIN      
        1 aactgaatca gcaggcggac ggatgtcggg tttcggtcac cctttgacgt ggtctgcacc
       61 agtgactgca ccccgaaggt gacccgcagg cggtcaatgt tcgcggatgt aatggtgcgc
      121 gtcaccggct ttgccttcgt cacttccacg cccagtgcgg tttccgcccc ggaggactca
      181 aagccttcag gtggtgtctg ctcctgctcc ccggcgcgcc agaccgcggt cacaccgtgt
      241 atcacaggat tgccgtccgt gtccgtcagc ggggttttgt tcaccaggat actctgcagc
      301 cccttcaccg ggccttcaat cggcccttca ccaatggcat caatcacgct catcatctgc
      361 gtggacttaa gattgtcctt tgcctctacc ggcgtgtgcg ccttgccgcc acctttgccc
      421 attgtctcac cctttactgt gataactgtt acgcacaaaa acaacaggca tcccggagga
      481 tgcctgtatc atgactgaat aaaaattctg aatatcttca cattttcaca aactgactgt
      541 ggcgctaata atttctctgc gttaatg
//
LOCUS       DAZFXT010000431          560 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.434, whole genome shotgun sequence.
ACCESSION   DAZFXT010000431 DAZFXT010000000
VERSION     DAZFXT010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..560
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.434"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            125..277
                     /locus_tag="ACHKW0_005372"
                     /pseudo
     CDS             125..277
                     /locus_tag="ACHKW0_005372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
     gene            complement(302..559)
                     /locus_tag="ACHKW0_005373"
     CDS             complement(302..559)
                     /locus_tag="ACHKW0_005373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001349388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477982.1"
                     /translation="MSFILVINNTYVNINIGAKRIRDTGCKAIYFVLISLGLTLFSII
                     SAAIEGVRCESLSVQLNVAWILFTTIMLSAIPSAKMSLSAH"
BASE COUNT          175 a          107 c          104 g          174 t
ORIGIN      
        1 gtaaaaaaat atttaaaaaa gaagtaaact tcacattttt tcattacatc tctgaagaga
       61 acctcgttaa tgattggtct tgctcattgt taataacaaa atgttgaatt taccgattag
      121 gaaacttttt cctacgcgga ttgcctccag ctatggcatg aactttgagt ttatgccaga
      181 actgaagtgg ggcttcggct accctggcgc gattatcttt atgatcctcg cgggcctgac
      241 atcgtatctg tactttaagc gtaagaactg gctgtaacta tacgatgtcg ctgtgagtaa
      301 gttagtgtgc tgacagcgac attttagccg agggaatcgc agataacata attgttgtga
      361 ataatatcca tgccacgttg agttggacgc ttaatgactc acatctcacc ccttcaattg
      421 ctgcagagat aatagaaaaa agtgtcaagc ccaatgaaat caatacaaag tagattgcct
      481 tacatccagt atctcttatt ctttttgctc caatattgat atttacatag gtattattaa
      541 taaccagaat aaaactcaaa
//
LOCUS       DAZFXT010000432          549 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.435, whole genome shotgun sequence.
ACCESSION   DAZFXT010000432 DAZFXT010000000
VERSION     DAZFXT010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.435"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            12..386
                     /locus_tag="ACHKW0_005374"
     CDS             12..386
                     /locus_tag="ACHKW0_005374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly chaperone"
                     /protein_id="HFO7477983.1"
                     /translation="MAKDLKTLALARLSGFRHKTVKVPEWRNVSVVLREPSAEAWYLW
                     QEVLNGDGEDDDTLSVVAKTRRNLEADVTLFCDVLCDTDLQRVFTPDDREQVLAVYGP
                     VHARLLRQALELIADAESARKK"
     gene            410..>549
                     /locus_tag="ACHKW0_005375"
     CDS             410..>549
                     /locus_tag="ACHKW0_005375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4035 domain-containing protein"
                     /protein_id="HFO7477984.1"
                     /translation="MRLALRLGRTLSELRHSLSASEAMMWMEFDRVSPLGDERGDIRN
                     AQ"
BASE COUNT          116 a          130 c          189 g          114 t
ORIGIN      
        1 ggataaggct tatggcaaaa gatctgaaaa cactggcgct ggccagactg tcggggttcc
       61 gtcataaaac ggtgaaggtg ccggaatgga gaaatgtcag cgtggtgctg cgggagcctt
      121 cggcagaggc ctggtatctg tggcaggaag tgctcaatgg tgatggagag gatgacgata
      181 ccctgtcggt ggtggcgaaa acccgccgta acctggaagc ggatgtgacg ctgttctgcg
      241 atgtcctgtg tgatactgac ctgcaacggg tgttcactcc ggacgaccgt gagcaggtgc
      301 tggccgtcta tggtccggta catgcccggt tgctgcgtca ggcactggaa ctgatcgctg
      361 atgcagagtc ggccagaaaa aagtagcccg cccggaaatt cgctttctga tgcgacttgc
      421 gctccgtctg gggcgcacct tatccgaact gcggcacagc ctgagtgcga gcgaggcgat
      481 gatgtggatg gagttcgaca gggtatcccc gctgggtgat gagcgcgggg atatccgtaa
      541 tgcacagat
//
LOCUS       DAZFXT010000433          538 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.436, whole genome shotgun sequence.
ACCESSION   DAZFXT010000433 DAZFXT010000000
VERSION     DAZFXT010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.436"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>538)
                     /locus_tag="ACHKW0_005376"
     CDS             complement(<1..>538)
                     /locus_tag="ACHKW0_005376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7477985.1"
                     /translation="QTSRIGSIGVMMSHVSYAGHLAQAGVDITLIYSGTHKVDGNQFE
                     ALPAEVRQDMQQRIDAARRMFAEKVAMFTGLSVDAVTGTEAAVFEGQSGIDAGLADEL
                     VNASDAISVMATALNSNVRGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLATML
                     AGQQGMSVEQARAILAAAA"
BASE COUNT          103 a          180 c          134 g          121 t
ORIGIN      
        1 tgccgccgcg gccagaatcg cccgggcctg ttcaacgctc atgccctgtt gtcctgccag
       61 catcgtggca agctgttcac gtcctttcgc ttcctggcat gtcaggatcc ccatcactcg
      121 ctggttctcc tgcgcggcgg cttccgttgc agttaattgc ggcatagtgc ctcctctgac
      181 attactgttc agcgccgtgg ccatcacact gatggcatcc gacgcattga ctaattcatc
      241 cgccagcccg gcatcaatgc cggactgacc ttcaaaaacg gcggcctctg ttcccgtgac
      301 ggcatcaaca gacagaccgg taaacatcgc cactttttcg gcaaacatcc ggcgcgccgc
      361 atcaatgcgc tgctgcatgt cctggcgaac ctctgccggt aaggcttcaa actgattgcc
      421 atccaccttg tgcgtccctg agtaaatcag cgtgatatcc acaccggcct gcgccagatg
      481 accggcatag ctgacatggc tcatcatcac gccaatggag ccgatacggg atgtctgg
//
LOCUS       DAZFXT010000434          535 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.437, whole genome shotgun sequence.
ACCESSION   DAZFXT010000434 DAZFXT010000000
VERSION     DAZFXT010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..535
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.437"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..236)
                     /locus_tag="ACHKW0_005377"
     CDS             complement(<1..236)
                     /locus_tag="ACHKW0_005377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HFO7477986.1"
                     /translation="MIETLLDFSGLEDISRDLQLLSGAENNRVLREATRAGANVLKEE
                     VVSRAPVRRGKLRRNVVVLSRRSRDGGMESGVHIR"
     gene            complement(233..>535)
                     /locus_tag="ACHKW0_005378"
     CDS             complement(233..>535)
                     /locus_tag="ACHKW0_005378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HFO7477987.1"
                     /translation="PVLTRNAAGEMTEEWVSCGKIHADIRGRSSRERMQSGAEMAQAE
                     IRIWVRGQSGREITAASRLHVLSGPWRDRILNVVGLPVPDATGGRLEILCRLGGEK"
BASE COUNT          105 a          195 c          127 g          108 t
ORIGIN      
        1 cggatatgca caccggattc catcccgcca tcgcgggagc gccgggaaag gaccaccaca
       61 ttgcggcgca gttttcccct gcgtaccggt gcccgtgaca ccacttcttc tttcagcaca
      121 ttcgcacccg cacgggttgc ctcacgcagc acccggttat tttctgcacc actcagaagc
      181 tgcaaatcgc ggctgatgtc ctccagcccc gaaaaatcca gcagggtttc gatcattttt
      241 cacctcccag ccgacagaga atttccagac gtccgccggt cgcatccggc acgggcagcc
      301 cgacaacgtt caggatccgg tcacgccagg gaccactcag cacatgaagt cgtgacgctg
      361 ccgtgatttc ccgaccggac tgaccgcgca cccagatgcg gatttccgcc tgcgccattt
      421 ccgcaccaga ctgcatccgc tcccggctgc tcctgccacg gatatccgca tgaattttcc
      481 cgcatgacac ccattcttcc gtcatttctc cggcagcatt acgggttaac accgg
//
LOCUS       DAZFXT010000435          527 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.438, whole genome shotgun sequence.
ACCESSION   DAZFXT010000435 DAZFXT010000000
VERSION     DAZFXT010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..527
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.438"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>527
                     /locus_tag="ACHKW0_005379"
     CDS             <1..>527
                     /locus_tag="ACHKW0_005379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HFO7477988.1"
                     /translation="IDKPFLLPIEDVFSISGRGTVVTGRVERGIIKVGEEVEIVGIKE
                     TQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPGTIKPHTKFESE
                     VYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVVTLIHP
                     IAMDDGLRFAIREGGR"
BASE COUNT          127 a          129 c          147 g          124 t
ORIGIN      
        1 attgacaagc cgttcctgct gccgatcgaa gacgtattct ccatctccgg tcgtggtacc
       61 gttgttaccg gtcgtgtaga acgcggtatc atcaaagttg gtgaagaagt tgaaatcgtt
      121 ggtatcaagg agactcagaa gtctacctgt actggcgttg aaatgttccg caaactgctg
      181 gacgaaggcc gtgctggtga gaacgtaggt gttctgctgc gtggtatcaa acgtgaagaa
      241 atcgaacgtg gtcaggtact ggctaagccg ggcaccatca agccgcacac caagttcgaa
      301 tctgaagtgt acattctgtc caaagatgaa ggcggtcgtc atactccgtt cttcaaaggc
      361 taccgtccgc agttctactt ccgtactact gacgtgactg gtaccatcga actgccggaa
      421 ggcgtagaga tggtaatgcc gggcgacaac atcaaaatgg ttgttaccct gatccacccg
      481 atcgcgatgg acgacggtct gcgtttcgca atccgtgaag gcggccg
//
LOCUS       DAZFXT010000436          526 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.439, whole genome shotgun sequence.
ACCESSION   DAZFXT010000436 DAZFXT010000000
VERSION     DAZFXT010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.439"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..193
                     /locus_tag="ACHKW0_005380"
     CDS             <1..193
                     /locus_tag="ACHKW0_005380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7477989.1"
                     /translation="ANTCMWDYRGDECGYNGPAVADEFDNPTTDIRKDRCSKCMRGCE
                     MRGMVANFGGFLSINKLSQ"
     gene            199..>526
                     /locus_tag="ACHKW0_005381"
     CDS             199..>526
                     /locus_tag="ACHKW0_005381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HFO7477990.1"
                     /translation="MTQTESAILAHARRCVPAESCGFVVRTPEGERYIPCVNISAEPE
                     AYFRIAPEDWLRAEMQGEIVALVHSHPGGLPWLSEADRRLQIKSALSWWLVCRGEIHK
                     FRCVPHL"
BASE COUNT          109 a          123 c          179 g          115 t
ORIGIN      
        1 ggcgaacacc tgtatgtggg attaccgggg agatgaatgc gggtataacg gtcctgcggt
       61 ggcggatgag ttcgacaacc ccaccacgga tatccgtaag gacagatgca gcaagtgcat
      121 gcgcgggtgt gagatgcgcg gcatggtggc taattttggc ggtttccttt ccattaacaa
      181 actttcgcag taaatccaat gacacagaca gaatcagcga ttctggcgca tgcccggcgg
      241 tgtgtgcctg cggagtcgtg cggcttcgtg gtgagaacgc cggaggggga gcggtatatc
      301 ccttgtgtga atatctctgc agagccggag gcgtattttc gtattgcacc ggaagactgg
      361 ctgcgggcag agatgcaggg ggagattgtg gcactggtcc acagtcatcc cggtggtctg
      421 ccctggctga gcgaggccga ccggcggctg cagataaaaa gtgcactgtc ctggtggctg
      481 gtctgccggg gggaaattca taaattccgc tgtgtgccac atctga
//
LOCUS       DAZFXT010000437          524 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.440, whole genome shotgun sequence.
ACCESSION   DAZFXT010000437 DAZFXT010000000
VERSION     DAZFXT010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.440"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>524
                     /locus_tag="ACHKW0_005382"
     CDS             <1..>524
                     /locus_tag="ACHKW0_005382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000211299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HFO7477991.1"
                     /translation="VGIAEYLANFMDKKVLIIDIDPQFNATQSILSKYDRVKEYIADH
                     LENTKTIRRIFETKSSLTGKVQQVTPDEIIIKMSEHLDMIFGDINTIFDIEQPAARLH
                     KLKRFIEEHKLRDIYDFILIDSPPTISLFTDAALVASDYYIVPVKIDHYSILGATSLL
                     SVIENIQDNYDHKN"
BASE COUNT          193 a           90 c           84 g          157 t
ORIGIN      
        1 gtcggcattg ctgaatatct tgcaaacttc atggataaaa aggtattaat tatcgatatt
       61 gacccacaat ttaacgctac acagtcaata cttagtaaat atgatcgagt gaaagagtat
      121 atagctgatc acttagaaaa tacaaaaacc atcagacgaa tattcgagac aaaatcaagc
      181 ttaacgggta aagttcagca ggtcactcct gatgaaatta tcatcaagat gtcagagcac
      241 ttagatatga tttttggtga tattaataca atatttgata ttgaacaacc tgcggcaaga
      301 ttacataagc taaaacgctt tatagaagaa cataagcttc gagatatata tgacttcatt
      361 ttgatcgata gcccaccaac catttcatta tttactgatg cagctctcgt tgcttcagat
      421 tattatattg tgccagttaa aattgaccac tactcaattt taggtgcgac aagtctttta
      481 agcgtaatag aaaacattca agataattat gaccataaaa acat
//
LOCUS       DAZFXT010000438          518 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.441, whole genome shotgun sequence.
ACCESSION   DAZFXT010000438 DAZFXT010000000
VERSION     DAZFXT010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.441"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            126..518
                     /gene="ydeI"
                     /locus_tag="ACHKW0_005383"
     CDS             126..518
                     /gene="ydeI"
                     /locus_tag="ACHKW0_005383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HFO7477992.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT          155 a          104 c          122 g          137 t
ORIGIN      
        1 gtatatttga cagctagttg gctgatttta aatgccttat aacaattggc aatattctta
       61 tttggcaatt ataaaaatag caaccagact taataagtca aaccaaacgc aattaaggag
      121 tcagtatgaa atttcaggcc attgtattag caagttttct tgtcatgcct tatgcgttgg
      181 cagatgacca gggcggttta aaacaggatg cagcgccacc gccgcctcat gcaatagaag
      241 atggctatcg cggaaccgat gatgcaaaaa aaatgaccgt tgatttcgca aaaaacatgc
      301 acgatggcgc ttcggtttca ctacgcggta atttgatttc tcacaaagga gaggaccgtt
      361 acgtttttcg cgataagagc ggtgaaatta atgtcgttat tcctgcggcc gtctttgatg
      421 gacgagaagt tcagccggac cagatgatca acattagcgg cagtctggat aagaaaagcg
      481 cgccggccgt cgttcgggtc actcatttgc agaaataa
//
LOCUS       DAZFXT010000439          508 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.442, whole genome shotgun sequence.
ACCESSION   DAZFXT010000439 DAZFXT010000000
VERSION     DAZFXT010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.442"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>508
                     /locus_tag="ACHKW0_005384"
     CDS             <1..>508
                     /locus_tag="ACHKW0_005384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HFO7477993.1"
                     /translation="VPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSALG"
BASE COUNT          129 a          121 c          162 g           96 t
ORIGIN      
        1 ctgtaccgcc atcgaaacgg gcacatacag gcagagtggc cgtgaaagaa agcaatcagc
       61 gatggtgctc tgacgggttc gagttctgct gtgataacgg agagagactg cgtgtcacgt
      121 tcgcgctgga ctgctgtgat cgtgaggcac tgcactgggc ggtgactacc ggcggcttca
      181 acagtgaaac agtacaggac gtcatgctgg gagcggtgga acgccgcttc ggcaacgatc
      241 ttccgtcgtc tccagtggag tggctgacgg ataatggttc atgctaccgg gctaatgaaa
      301 cacgccagtt cgcccggatg ttgggacttg aaccgaagaa cacggcggtg cggagtccgg
      361 agagtaacgg aatagcagag agcttcgtga aaacgataaa gcgtgactac atcagtatca
      421 tgcccaaacc agacgggtta acggcagcaa agaaccttgc agaggcgttc gagcattata
      481 acgaatggca tccgcatagt gcgctggg
//
LOCUS       DAZFXT010000440          506 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.443, whole genome shotgun sequence.
ACCESSION   DAZFXT010000440 DAZFXT010000000
VERSION     DAZFXT010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.443"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..176)
                     /locus_tag="ACHKW0_005385"
     CDS             complement(<1..176)
                     /locus_tag="ACHKW0_005385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HFO7477994.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVV"
     gene            complement(234..>506)
                     /locus_tag="ACHKW0_005386"
     CDS             complement(234..>506)
                     /locus_tag="ACHKW0_005386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HFO7477995.1"
                     /translation="TGSSGISSATPALTPLMLDEATGKLVVWDGQKAGSAVGILVLPL
                     EGTETVLTYYKSGTFATEAIRWPESVDEHKKANAFAGSALSHAALP"
BASE COUNT          111 a          150 c          124 g          121 t
ORIGIN      
        1 tcaacaaccg gggagacata ggccgccacc ggcgtttttc cggtaatttt gtccagcatc
       61 acctcttcgg tatggaaatt caccgtacgg cggaaaaaca gctccagaaa cagcgcacga
      121 aatttcactt tttgttcggt ataaccgagt aactggcggg tcgtaaacaa tcccataaat
      181 cagttccttt cattcagaaa tcagtcaggc caccgcggtg gcctgataac gtgttacggc
      241 agcgccgcgt gactcagggc actgccggca aaggcatttg cctttttgtg ttcatccaca
      301 ctttcaggcc agcggattgc ctccgtcgca aaggtccccg acttgtaata ggtcagtacc
      361 gtctctgtgc cttcaagcgg cagtaccagt atgccaactg cactaccggc tttctgtccg
      421 tcccagacca ccagtttccc ggtagcttca tccagcatca ggggcgtcag tgccggtgtt
      481 gccgaggaaa tcccgctgct gcctgt
//
LOCUS       DAZFXT010000441          503 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.444, whole genome shotgun sequence.
ACCESSION   DAZFXT010000441 DAZFXT010000000
VERSION     DAZFXT010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.444"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            1..137
                     /locus_tag="ACHKW0_005387"
                     /pseudo
     CDS             1..137
                     /locus_tag="ACHKW0_005387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_303550.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(332..445)
                     /locus_tag="ACHKW0_005388"
     CDS             complement(332..445)
                     /locus_tag="ACHKW0_005388"
                     /inference="COORDINATES: protein motif:HMM:NF024464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HFO7477996.1"
                     /translation="MVDFTSRIMSVLADGVLVCGIMVLLWPVIKRNSLHNA"
BASE COUNT          157 a          130 c          111 g          105 t
ORIGIN      
        1 taatgtttaa tcgacatcga atttctcccg tgtagaggaa cagagttaaa aagccggaag
       61 cggaatcaaa tcacaggatg accatctgcc agtggctggt cgtaaaaaaa aggccgcgcc
      121 atgcgcagcc gaaaataaag ggataacgat gatagtttga gaaaaacaga aataacactt
      181 ttgtggcaaa gcatggtgcc gggtgcctcc cggtgaattc agtatcagca cctgaatccg
      241 cgattacccc atattccttc ttgctgattg ccccaccgca cagggggatt caccatgcag
      301 aagtgttttt aataaacagc aaacaaaaaa atcaagcatt atgcaggctg tttcttttta
      361 tcaccggcca cagcaatacc ataatgccgc agaccagcac cccatccgcc agcaccgaca
      421 tgattctgct ggtgaaatcc accatcacca ccagaaacag caggagtgca gccacagtca
      481 ggcgcagttt taccgtcaca ggt
//
LOCUS       DAZFXT010000442          501 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.445, whole genome shotgun sequence.
ACCESSION   DAZFXT010000442 DAZFXT010000000
VERSION     DAZFXT010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.445"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..244)
                     /locus_tag="ACHKW0_005389"
     CDS             complement(<1..244)
                     /locus_tag="ACHKW0_005389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477997.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDV"
     gene            complement(317..>501)
                     /locus_tag="ACHKW0_005390"
     CDS             complement(317..>501)
                     /locus_tag="ACHKW0_005390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7477998.1"
                     /translation="SEQVYRVWRKAAEKTTDQTLIRKLPDGEIRRYDRHQNIICSECR
                     NSEVMQRVLAFCQGNF"
BASE COUNT          120 a          138 c           93 g          150 t
ORIGIN      
        1 ttacatcatc tgccagtctg gtttctcttt tcgccacaga gagcatccag tcaaatggct
       61 ccacaactgc accgcagatt ttacagcgga cctgacgctc tttttcgtca acccggacag
      121 aggcgtgatg gcaatatggt ctttccgatg gctcataaag aaaattaacc tgattacgtg
      181 ggtcatcctc ttttaccgga aataaaacaa tattacttaa ctcatcttct ggttttattt
      241 ccatgcacct ctcctttgat gcgaatgcca gcggcaattg aagcctgata gctaatttca
      301 ctcacagcac cacctcctaa aaattcccct gacagaacgc cagtacacgc tgcataactt
      361 cgctatttcg gcactcactg caaattatgt tctgatgcct gtcatagcgg cgtatttctc
      421 cgtctggtaa ctttcgaatc aatgtctggt cggttgtttt ctccgctgcc ttacgccata
      481 cgcgatacac ctgttctgat g
//
LOCUS       DAZFXT010000443          500 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.446, whole genome shotgun sequence.
ACCESSION   DAZFXT010000443 DAZFXT010000000
VERSION     DAZFXT010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.446"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            21..>500
                     /locus_tag="ACHKW0_005391"
     CDS             21..>500
                     /locus_tag="ACHKW0_005391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7477999.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSIFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQINNIRYEKNKSAECRIIVVANPKPDKAIITKISVEEGIPITFSV
                     "
BASE COUNT          199 a          102 c           77 g          122 t
ORIGIN      
        1 gatgatgaat aaggaatgtt atgtcaattg actttacccc aggtataata aatacatatc
       61 acggcgatat ttataactgc acaacaaata ccgataatgt taaaacgcca gacacaccga
      121 agtggccttg tgataattgg gaagaacaac aacccattaa ttccatattc tctggagaag
      181 gatacaaccc tgaacaattt gatttagcgc aacaacaact ccaacaaatt aatgcatgcc
      241 acacgaacac cacatataca aatgcagact actcaaaagt tgtggttcaa cttgtaagtc
      301 ttattaataa cattgaaacg ataagttcga caccacttac gcaacagaca caatcaatac
      361 ttaatcagat taataacatt agatatgaaa aaaacaaaag tgctgaatgt cgaatcattg
      421 ttgtcgctaa tcctaaaccc gacaaggcaa tcataactaa aattagcgta gaagagggaa
      481 tacccataac attcagtgtc
//
LOCUS       DAZFXT010000444          498 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.447, whole genome shotgun sequence.
ACCESSION   DAZFXT010000444 DAZFXT010000000
VERSION     DAZFXT010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.447"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          146 a           89 c          110 g          153 t
ORIGIN      
        1 gtactggagc tattagtggc tctgttacgg ttgattgatt tgttgaagta acgggcaaat
       61 tgaaacgcat ctaaggggga aaaccgcttc cccctttaat aaaatgatta ctgtttttat
      121 taattcatca tataactcat ttttatttat aaattcatcg attttatatt caacgcacca
      181 tttgaccggg atgtgaatat ttatggtggg ctattggcaa aaaatatctg gaaagtagat
      241 cgcagaaaat aaaaaaacct gctggtgagg caggttcttt tatttactac tttgatgtta
      301 agttactgat agtgcaacat ggcatctcaa cgaacgaatt ataaccacct ctggacagtg
      361 ttgcaattaa gataatgaga tttataatcc tgactgttgg gaagttgcgc cggatcgaac
      421 ccatttcaag tcctggctac cggacgggtg gtgccgcagg tagtgctctg atccggagtg
      481 caacatggcg actcaaca
//
LOCUS       DAZFXT010000445          486 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.448, whole genome shotgun sequence.
ACCESSION   DAZFXT010000445 DAZFXT010000000
VERSION     DAZFXT010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.448"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>486
                     /locus_tag="ACHKW0_005392"
     CDS             <1..>486
                     /locus_tag="ACHKW0_005392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HFO7478000.1"
                     /translation="GWRGGWSLYAYPLNPVNGIDPLGLSPADVALIRRKDQLNHQRAW
                     DILSDTYEDMKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRDYGLNL
                     FGMYGRKVKLSHSEMIEDNKKDLAVNDHGLTCPSTTDCSDRCSDYINPEHKKTIKALQ
                     DA"
BASE COUNT          164 a           68 c          123 g          131 t
ORIGIN      
        1 gggctggagg gggggatgga gtctgtatgc gtacccgctg aatccggtga atggtattga
       61 tccattaggg ttaagtcccg cagatgtagc gctaataaga agaaaagatc aactaaacca
      121 tcaaagagca tgggatatat tatctgatac ttatgaagat atgaagagat taaatttagg
      181 tgggactgat caatttttcc attgtatggc attttgtcga gtgtctaaat taaatgacgc
      241 tggtgttagc cgatcggcga aagggctggg ttatgaaaaa gagattagag attacgggtt
      301 aaatctgttc ggtatgtacg gcagaaaagt aaagctatcc cattctgaaa tgattgaaga
      361 taataaaaaa gacttggctg taaatgacca tgggttgaca tgtccatcaa caacagattg
      421 ctcagataga tgtagtgatt atattaatcc agagcataaa aaaacgataa aggctttaca
      481 agatgc
//
LOCUS       DAZFXT010000446          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.449, whole genome shotgun sequence.
ACCESSION   DAZFXT010000446 DAZFXT010000000
VERSION     DAZFXT010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.449"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            74..370
                     /locus_tag="ACHKW0_005393"
     CDS             74..370
                     /locus_tag="ACHKW0_005393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0060703 - deoxyribonuclease inhibitor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="host-nuclease inhibitor Gam family protein"
                     /protein_id="HFO7478001.1"
                     /translation="MNAYYIQDRLEAQSWARHYQQIAREEKEAELADDMEKGLPQHLF
                     ESLCIGHLQRHGASKKAITRAFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     gene            376..>485
                     /locus_tag="ACHKW0_005394"
     CDS             376..>485
                     /locus_tag="ACHKW0_005394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7478002.1"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKG"
BASE COUNT          125 a          124 c          127 g          109 t
ORIGIN      
        1 cccctttcct gttttcctaa tcagcccggc atttcgcggg cgatattttc acagctattt
       61 caggagttcg gccatgaacg cttattacat tcaggatcgt cttgaggctc agagctgggc
      121 gcgtcactac cagcagatag cccgtgaaga gaaagaggca gaactggcag acgacatgga
      181 aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc ggtcatttgc aacgccacgg
      241 ggccagcaaa aaagccatta cccgtgcgtt tgatgacgat gttgagtttc aggagcgcat
      301 ggcagaacac atccggtaca tggttgaaac cattgctcac catcaggttg atattgattc
      361 agaggtataa aacggatgag tacagcactc gcaacgctgg cagggaagct ggctgaacgt
      421 gtcggcatgg attctgtcga cccacaggaa ctgatcacca ctcttcgcca gacggcattt
      481 aaagg
//
LOCUS       DAZFXT010000447          485 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.450, whole genome shotgun sequence.
ACCESSION   DAZFXT010000447 DAZFXT010000000
VERSION     DAZFXT010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.450"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..485)
                     /locus_tag="ACHKW0_005395"
     CDS             complement(<1..485)
                     /locus_tag="ACHKW0_005395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001485704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HFO7478003.1"
                     /translation="MRVFHGGRVLVESEPKSMRNLPSGVVPAVRQPLAEDKSLLPFFS
                     NERVIRAAGGAGALSDWLLRHVKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAQQNLSAWMIDVIRHAMNGIQERELSLAELSWWAVCNQVVDALPEAVS
                     RR"
BASE COUNT          119 a          140 c          120 g          106 t
ORIGIN      
        1 cgacgcgata ctgcctcagg taatgcgtcc accacctgat tgcagactgc ccaccaggat
       61 aattcagcca gcgataattc ccgttcctgt atgccattca ttgcgtggcg gatgacgtca
      121 atcatccatg ctgacaggtt ttgttgagca agttgctcaa gtgattcgga tgtctggtcg
      181 cgcagctggt tgtcgcagtg ccagcacaac accatcgcgc cggtaccgta acgatgtatg
      241 acagtttcgc tgtgatgata atcaccatga ggccactggc aggatttaac gtgacgtaat
      301 aaccagtcag acagtgcacc agcgccacca gcagcacgaa tcacacgctc attgctgaaa
      361 aatggcagta atgatttatc ttccgccagc ggctggcgaa cggcaggaac gaccccggac
      421 ggcagattac gcatgctttt cggttcagac tccaccagca ccctgccgcc atgaaatacc
      481 cgcat
//
LOCUS       DAZFXT010000448          484 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.451, whole genome shotgun sequence.
ACCESSION   DAZFXT010000448 DAZFXT010000000
VERSION     DAZFXT010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.451"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>484
                     /locus_tag="ACHKW0_005396"
     CDS             <1..>484
                     /locus_tag="ACHKW0_005396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HFO7478004.1"
                     /translation="DKAYKLTDGAGMFLLVHPNGSRYWRLRYRILGKENTLALGVYPE
                     VSLSEARTKRDEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIARRWHASNKQWAQSH
                     SDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEIASRLQQRISAVMRY
                     A"
BASE COUNT          136 a          120 c          105 g          123 t
ORIGIN      
        1 ctgacaaggc ttataaactc actgacgggg ctggcatgtt cctgctggtt catcctaatg
       61 gttcccgtta ctggcgtctc cgttatcgta ttctgggtaa ggagaatact ctggcacttg
      121 gtgtgtatcc agaagtttct ctctccgaag ctcgtacaaa acgggatgag gcccgaaaac
      181 tgatttcaga ggggattgac ccttgcgaac agaaaagagc taaaaaagta gtccctgatt
      241 tacaactctc ttttgaacat attgcacgac gctggcatgc cagtaataaa caatgggcac
      301 aatcacacag cgataaggta ctcaaaagcc tcgaaacaca cgttttcccc tttatcggca
      361 accgggatat cacaacactc aataccccgg acctgcttat ccctgttcgt gctgcagaag
      421 ctaaacaaat ttatgaaatc gccagtcgtc tgcagcaaag aatatctgct gtaatgcgtt
      481 atgc
//
LOCUS       DAZFXT010000449          483 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.452, whole genome shotgun sequence.
ACCESSION   DAZFXT010000449 DAZFXT010000000
VERSION     DAZFXT010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.452"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..393
                     /locus_tag="ACHKW0_005397"
     CDS             <1..393
                     /locus_tag="ACHKW0_005397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310263.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HFO7478005.1"
                     /translation="AGKGGLQIVLGAAAIVGSFFTAGATMALWGSALAAGGFSATTML
                     FSLGASMILGGVAQMLAPKAKTPEYKSTDNGKQNTYFSSLDNMIAQGNPMPVPYGEML
                     VGSRRISQDISTRDEGGGGKVVVIGRQG"
BASE COUNT          120 a          111 c          156 g           96 t
ORIGIN      
        1 gcagggaaag gcggactgca gattgtgctg ggggcggcag ccatcgtggg ctctttcttc
       61 accgccggcg caacgatggc gttatggggt tcagccctgg cagccggcgg tttttctgcc
      121 accacgatgc tgttttcact gggggccagc atgatactgg gcggtgtggc ccagatgctg
      181 gccccgaagg caaagacgcc ggagtacaaa agtacggata acggtaaaca gaacacgtat
      241 ttttcgtcac tggacaacat gattgcccag gggaacccga tgccggtgcc ttacggtgaa
      301 atgctggttg gctcccggcg aatctcccag gacatcagca cccgtgatga aggtggtggc
      361 gggaaggtgg tggttatcgg gcggcagggg taaaaagaat aaaaaaatcc cgcagagttg
      421 cggagctgcg ggaagagata caaagattaa ctatagagaa ttattcttat gtcacgacaa
      481 aaa
//
LOCUS       DAZFXT010000450          480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.453, whole genome shotgun sequence.
ACCESSION   DAZFXT010000450 DAZFXT010000000
VERSION     DAZFXT010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.453"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>480
                     /locus_tag="ACHKW0_005398"
     CDS             <1..>480
                     /locus_tag="ACHKW0_005398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
                     /protein_id="HFO7478006.1"
                     /translation="AIEAGDERRARAKFHWQFLEHYPAAQDCAYKFLVCEDKPDIPRP
                     ALDSWDAEYMQENRWDEESASFVPVETESDLMNVTFDKLAPEVQNALMVKFDTCENIT
                     VDMVISAQELLQEDMATFNGHIVEALMKMPEVNAMCPELKLHAIGWVKHKCKPGAKWP
                     "
BASE COUNT          124 a          113 c          122 g          121 t
ORIGIN      
        1 gccattgaag ccggtgatga acgtcgcgcc cgcgcaaaat ttcactggca attcctggaa
       61 cattatccgg ctgctcagga ctgcgcttat aaatttcttg tttgcgagga taaacccgat
      121 attccccgcc ctgccctcga ttcctgggat gctgaatata tgcaggaaaa ccgctgggat
      181 gaggagtctg cttcttttgt cccggttgag actgaatccg atctgatgaa cgtcactttt
      241 gacaagctgg cccctgaagt acagaacgct ctcatggtta agttcgacac atgtgaaaac
      301 atcaccgttg atatggttat tagcgcacag gaattgttgc aggaagacat ggcaacattc
      361 aacggacata tcgttgaagc gttgatgaaa atgccagaag ttaacgccat gtgtccggag
      421 cttaagctgc atgccatcgg gtgggttaag cataaatgta agcctggtgc caaatggccc
//
LOCUS       DAZFXT010000451          480 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.454, whole genome shotgun sequence.
ACCESSION   DAZFXT010000451 DAZFXT010000000
VERSION     DAZFXT010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.454"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..366
                     /locus_tag="ACHKW0_005399"
     CDS             <1..366
                     /locus_tag="ACHKW0_005399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HFO7478007.1"
                     /translation="GPWQSHPKRMLRHKAMIQCARLAFGFAGIYDKDEAERIVENTAY
                     TAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLPLCSQIFRRDIRASSELTQAE
                     AVKALGFLKQKATEQKVAA"
     gene            363..>480
                     /locus_tag="ACHKW0_005400"
     CDS             363..>480
                     /locus_tag="ACHKW0_005400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO7478008.1"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHN"
BASE COUNT          130 a          122 c          129 g           99 t
ORIGIN      
        1 gggccgtggc agtcgcatcc taaacggatg ttacggcata aagctatgat tcagtgtgcc
       61 cgtctggcct tcggatttgc tggtatctat gacaaggatg aagccgagcg cattgtcgaa
      121 aatactgcat acactgcaga acgccagccg gaacgcgaca tcactccggt taacgatgaa
      181 accatgcagg agattaacac tctgctaatc gccctggata aaacatggga tgacgactta
      241 ttgccgctct gttcccagat atttcgccgc gacattcgcg catcgtcaga actgacacag
      301 gccgaagcag tgaaagctct tggattcctg aaacagaaag ccactgagca gaaggtggca
      361 gcatgacacc ggacattatc ctgcagcgta ccgggatcga cgtgagagct gtcgaacagg
      421 gggatgatgc gtggcacaaa ttacggctcg gcgtcatcac agcttcagaa gttcataacg
//
LOCUS       DAZFXT010000452          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.455, whole genome shotgun sequence.
ACCESSION   DAZFXT010000452 DAZFXT010000000
VERSION     DAZFXT010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.455"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          140 a          100 c          101 g          132 t
ORIGIN      
        1 accttgtgct gccgcagcca tagggaacgc aagttgagtt atcaggcaga tacccgcagt
       61 taagcggcgt agtttaattc ccatcccgca taagatttgt cggtcattta tttcttctcc
      121 acttctcttc ttcgtagcca tgcttacctt ccctgaacga gctaacataa atgcgccctt
      181 tctagcacag cgaaaaaatg taaatcctga tagcaatatg attgaaaatt aataactttt
      241 cgttaggcag ttttgggtgt gagttgcaag aggggagact actgaataac tcaagtttta
      301 taatcgaggg gaaaatggtg atggcgttca tagcaaaacg ccctcaacca taaaggtcga
      361 gggcgcttaa gatgttaaaa acccgctatc cgttaaaaaa caatgttcaa ctaaggtcag
      421 tgacattgcg ctaaaaaagc gaatttttag aatttggctc ctctgactgg act
//
LOCUS       DAZFXT010000453          473 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.456, whole genome shotgun sequence.
ACCESSION   DAZFXT010000453 DAZFXT010000000
VERSION     DAZFXT010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.456"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..278)
                     /locus_tag="ACHKW0_005401"
     CDS             complement(<1..278)
                     /locus_tag="ACHKW0_005401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HFO7478009.1"
                     /translation="MFGIFKKKTRRAAAEIKKFEKRDLAQAVINAAYLVAYADGECEA
                     SEKAKIEQVLRNQPALSAFTSEINAISATIIGQLDTNFKIGRRAALRE"
BASE COUNT          113 a          128 c          119 g          113 t
ORIGIN      
        1 atctcgcgta acgcggcacg acgaccaatt ttaaaattgg tatccagctg accgataata
       61 gttgcgctaa tcgcattaat ttccgaggta aacgcagaca acgcaggctg attacgtaag
      121 acctgttcga tcttcgcttt ctcggatgcc tcgcattcac catctgcata ggccaccagg
      181 tatgcagcgt taatcaccgc ctgtgccaga tcgcgtttct caaacttttt aatttccgct
      241 gccgctctgc gggttttctt tttgaagatt ccaaacatcg tgacgttcct ttgggtgggt
      301 gagccaacgc ccgggagcga tctgcccaca gagaaagtca cactgaccac tccgtaagct
      361 cacccccgaa aggctctgtg gttgatatgc gccgggcgtg gcgcagatac aaaaaaggcc
      421 cgccgaagcg agcctggaaa ataagtgtgg cgcgttgtag tggagtcgaa cca
//
LOCUS       DAZFXT010000454          467 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.457, whole genome shotgun sequence.
ACCESSION   DAZFXT010000454 DAZFXT010000000
VERSION     DAZFXT010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.457"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>467
                     /locus_tag="ACHKW0_005402"
     CDS             <1..>467
                     /locus_tag="ACHKW0_005402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478010.1"
                     /translation="LSVNRLVYFSLLKKLFQAMNLCQIPGRAGALLTTLLRHISTING
                     FDNLMAQPLLSDEPLTALMDHYLDTDALADGLPLYVSLYPTEGGMQDIIDCIRAELGV
                     GTTKNAVFQHIQSLPRGQQKEALLASAALPLLFRPREVQGTMFGDGGMGGWRN"
BASE COUNT          103 a          117 c          130 g          117 t
ORIGIN      
        1 ctgtcggtaa acagattggt ttacttttca ttgctgaaaa aattgttcca ggcaatgaac
       61 ctctgccaga tccccggacg tgcaggagca ctgcttacga cgcttcttcg ccatatatcg
      121 acaatcaacg ggtttgacaa tctgatggct cagccgttgt tgtcagatga gcccctgaca
      181 gcgctgatgg atcattatct tgatactgat gctctggcag acgggctacc gctgtatgtg
      241 tcgctgtacc ccacagaagg gggcatgcag gatattattg actgcattcg tgctgaactg
      301 ggtgtcggaa ccacgaaaaa cgccgttttt cagcatatcc agagcctgcc ccgcggacag
      361 cagaaagagg ctctgcttgc gtcagccgcg ctgcccctgc tgttccgtcc ccgtgaggtt
      421 caggggacaa tgttcggtga tggtggtatg ggaggatggc gaaatat
//
LOCUS       DAZFXT010000455          466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.458, whole genome shotgun sequence.
ACCESSION   DAZFXT010000455 DAZFXT010000000
VERSION     DAZFXT010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.458"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..155
                     /locus_tag="ACHKW0_005403"
     CDS             <1..155
                     /locus_tag="ACHKW0_005403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HFO7478011.1"
                     /translation="TDQTLIRKLPDGEIRRYDRQQNIICGECRKSEVMLRVLAFYQGN
                     FQEAIL"
     gene            213..>466
                     /locus_tag="ACHKW0_005404"
     CDS             213..>466
                     /locus_tag="ACHKW0_005404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478012.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEASERAYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLR"
BASE COUNT          141 a           89 c          123 g          113 t
ORIGIN      
        1 caaccgacca gacattgatt cgaaagttac cagacggaga aatacgccgc tacgacagac
       61 aacagaacat aatctgtggc gagtgccgga agagtgaagt tatgctgcgt gtactggctt
      121 tctatcaggg taattttcag gaggcgatac tgtgaatgaa attagctatc aggcttcaat
      181 taccgctggc attcacatca aaggagaaga gcatggaaat aaaaccagaa gatgagttaa
      241 gcaatatcgt tttatttccg gtaaaagagg atgacccacg taatcaggtt aattttcttt
      301 atgaggcatc ggaaagagca tattgtcatc acgcctctgt tcgggttgac gaaaaagagc
      361 gtcaggtccg ctgtaaaatc tgcggtgcag ttgtggagcc gtttgactgg atgctctctg
      421 tggcgaaaag agaaaccaga ctggcagatg atgtaaggct cttgcg
//
LOCUS       DAZFXT010000456          466 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.459, whole genome shotgun sequence.
ACCESSION   DAZFXT010000456 DAZFXT010000000
VERSION     DAZFXT010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..466
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.459"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>466)
                     /locus_tag="ACHKW0_005405"
     CDS             complement(<1..>466)
                     /locus_tag="ACHKW0_005405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HFO7478013.1"
                     /translation="DVEWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAI
                     EMLRDPDWVSKITHLDQDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTV
                     FIHYVGPTKPWHEWADYPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKH"
BASE COUNT          140 a           96 c           75 g          155 t
ORIGIN      
        1 ttatgttttg cacaataccg atactgattg ctatttacag gtttaagtaa atcttcttta
       61 ctccacggag aagctgcttt ggcgatcaaa aaactacgtg acaccggata gtcagcccac
      121 tcatgccagg gttttgtagg tcccacatag tgtataaaca cggtgtcatc attgactgga
      181 ttatcaactt tgtcttttaa ttcatagtta atactatatc gggtattata tttctccgaa
      241 ataaacttca ctttaccatt caataataca ttcagtacat cttgatcaag gtgggtgatt
      301 ttacttaccc aatctgggtc acgcaacatt tcaatagctt ttgacgaaat gttatttaga
      361 ttccactcat caatatttat cagtaaaaaa ccagcattga aatatccaga agctaactgt
      421 ggtgtagtta ataccgaggc tcgattttgc caccattcaa catctc
//
LOCUS       DAZFXT010000457          463 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.460, whole genome shotgun sequence.
ACCESSION   DAZFXT010000457 DAZFXT010000000
VERSION     DAZFXT010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..463
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.460"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>463
                     /locus_tag="ACHKW0_005406"
     CDS             <1..>463
                     /locus_tag="ACHKW0_005406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001239084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TcdA/TcdB catalytic glycosyltransferase
                     domain-containing protein"
                     /protein_id="HFO7478014.1"
                     /translation="IKLQDNFFNYCIVKGVTEINDELRINYLKNVIKLSDDDIGNYQK
                     TINDNKDRVKKLILDLQKQFGENRISIKDVNSLTSLSKSENNHNYQTEMLLRWNYPAA
                     SDLLRMYILKEHGGIYTDTDMMPAYSKQVIFKIMMQTNGDNRFLEDLKLRRA"
BASE COUNT          177 a           75 c           74 g          137 t
ORIGIN      
        1 gattaaactt caggataatt tcttcaacta ttgcattgtc aaaggtgtta cagagattaa
       61 tgatgagtta cgaataaact accttaaaaa tgtaataaaa ctgtcagacg atgacattgg
      121 taattatcag aaaacaatta acgacaataa agatagagta aaaaaactaa ttcttgattt
      181 acagaaacaa tttggtgaaa accgcatttc aattaaagat gttaactctt taacctctct
      241 ttcaaaatca gaaaataatc acaattatca aactgaaatg ttgcttcgat ggaactatcc
      301 tgccgcctca gacctgctca ggatgtatat ccttaaagag catggtggta tttatacaga
      361 cacagatatg atgcctgcat actctaaaca agtaattttt aaaattatga tgcagacaaa
      421 cggagataat cgtttcttgg aagatttgaa actacgtcgt gca
//
LOCUS       DAZFXT010000458          459 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.461, whole genome shotgun sequence.
ACCESSION   DAZFXT010000458 DAZFXT010000000
VERSION     DAZFXT010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..459
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.461"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..182
                     /locus_tag="ACHKW0_005407"
     CDS             <1..182
                     /locus_tag="ACHKW0_005407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7478015.1"
                     /translation="GWFPDTDGALITGLTFLDPKDATQVQGLFQHLQVRFGDGPWQDV
                     KGLNEVGSDTGRTGE"
     gene            184..453
                     /locus_tag="ACHKW0_005408"
     CDS             184..453
                     /locus_tag="ACHKW0_005408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail fiber C-terminal domain-containing
                     protein"
                     /protein_id="HFO7478016.1"
                     /translation="MNILKKLMQRLCGCGKHDGREHVQSLTAQLRLGPADILESDENG
                     IIPEQDRVITQVVILDADKKQIQCVVRPLQILRADGTWENIGGMK"
BASE COUNT          126 a           93 c          145 g           95 t
ORIGIN      
        1 atggctggtt cccggataca gatggcgcac tcatcaccgg actgaccttt cttgacccca
       61 aagatgccac acaggttcag gggctgtttc agcatttgca ggtcaggttt ggtgacgggc
      121 cgtggcagga tgttaagggg ctgaatgaag tgggcagtga tacaggcaga acaggagaat
      181 gacatgaata tactaaaaaa acttatgcag cgtctgtgtg gttgcggaaa gcatgatggc
      241 cgtgaacacg tgcagtcgct tacagcacaa ctgcgactgg gaccggcaga cattctggag
      301 tcagatgaga atggcattat tccggagcag gacagggtaa tcacacaggt ggtgatactg
      361 gatgcggata aaaagcagat acagtgcgtg gtaagaccgc tgcaaattct gcgtgctgac
      421 gggacgtggg aaaatattgg cgggatgaag taacccgac
//
LOCUS       DAZFXT010000459          455 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.462, whole genome shotgun sequence.
ACCESSION   DAZFXT010000459 DAZFXT010000000
VERSION     DAZFXT010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.462"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>455)
                     /locus_tag="ACHKW0_005409"
     CDS             complement(<1..>455)
                     /locus_tag="ACHKW0_005409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7478017.1"
                     /translation="SEHQSTLSAGYLHASTNVPGSDDLNGINVKYRYEFTDTLGMVTS
                     FSYAGDKNRQITRYSDTRWHEDSVRNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVST
                     FSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPIESVAID"
BASE COUNT          104 a          139 c          111 g          101 t
ORIGIN      
        1 atatcaatgg ccacggattc gatcgggtta aactgcacgc cagctcccca cgccagagac
       61 gtgttgctgt ggcgaccgtc atcacttccg gtcagcacat catgcgtttt ccccttgttg
      121 tcagttacgc ggagataatc cccggaaaaa gtcgacacac ggctgtaagc cacgcccgcc
      181 atcgcatacg cactgaacca ttcattcacg cgcacagagg gccccgccat cacgctgaac
      241 cagcggttac gaacggaatc ttcatgccag cgggtatcgc tgtaacgggt aatctggcga
      301 ttcttgtctc ctgcatagct gaatgacgtc accatcccca gtgtgtccgt aaactcataa
      361 cggtatttca cgttaatccc gttcagatca tcgctgccgg gaacgttcgt cgaggcatga
      421 agataccccg cgctcagcgt ggactgatgt tcaga
//
LOCUS       DAZFXT010000460          450 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.463, whole genome shotgun sequence.
ACCESSION   DAZFXT010000460 DAZFXT010000000
VERSION     DAZFXT010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.463"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..363
                     /locus_tag="ACHKW0_005410"
     CDS             <1..363
                     /locus_tag="ACHKW0_005410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6645 domain-containing protein"
                     /protein_id="HFO7478018.1"
                     /translation="ALTGDGGNNLLAAFYIQTDAKDLNVMYHNAKVATNNLKLGTFGA
                     FDNEWHTLAFRFAGNNSLQVTPVIDGQDGTPFTLTQSPVSAFAADKLHVTDITRNATY
                     PVLIDSIVVEVNSTDTAA"
BASE COUNT          130 a          104 c          127 g           89 t
ORIGIN      
        1 gccctgaccg gagacggggg gaataacctg ctggcagcgt tctacatcca gacagatgca
       61 aaagacctga atgtgatgta ccacaatgcg aaagtggcga caaacaacct gaaactggga
      121 acctttggcg catttgataa cgaatggcat acgctggctt tccgctttgc cgggaataac
      181 agccttcagg tgacgccggt tattgatggt caggatggca caccgttcac gctgacgcag
      241 tcaccggtca gtgcatttgc ggcggataaa ctgcatgtga cagacattac caggaatgcg
      301 acttacccgg tactgataga cagcattgtg gtggaagtga acagcacaga cactgcggca
      361 tgataaaaaa accgccagcg acaggaatgg acgctggcgg tggtgatacc tatggagaaa
      421 aaataaagga acgatacttt cgtactctgg
//
LOCUS       DAZFXT010000461          442 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.464, whole genome shotgun sequence.
ACCESSION   DAZFXT010000461 DAZFXT010000000
VERSION     DAZFXT010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.464"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..317)
                     /gene="nleC"
                     /locus_tag="ACHKW0_005411"
                     /pseudo
     CDS             complement(30..317)
                     /gene="nleC"
                     /locus_tag="ACHKW0_005411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector zinc
                     metalloprotease NleC"
     gene            complement(351..>442)
                     /locus_tag="ACHKW0_005412"
     CDS             complement(351..>442)
                     /locus_tag="ACHKW0_005412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478019.1"
                     /translation="HNYNHFCDFIEFKHPNMIMNTSQYTCSSW"
BASE COUNT          143 a           81 c           87 g          131 t
ORIGIN      
        1 ccatagctta cggcatcacg aaaagtttat gagcggctca aagcatcaag tatagtatta
       61 ttaatcatat cgaccgtatg ctgatcaatg agtctgccac gactgtcatg gacggcacga
      121 attacacttt cgtatacgtt gcttaaatct gctgcggaaa gtggtattcg cttgcctata
      181 tccaaaacgt aatccgcata ggcattttca gcacgattag gagcaatgag agcagagtat
      241 cctgccgggg cggaaaagtt gaagttggac tgtaatgagg gaattttcat gttgatacct
      301 aataccatgc aaatcattta ttacaacaga ataaacttta tcaaattcat ttaccatgaa
      361 ctgcatgtat actgacttgt gttcatgatc atattagggt gtttaaactc aataaagtca
      421 caaaaatgat tataattatg ta
//
LOCUS       DAZFXT010000462          441 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.465, whole genome shotgun sequence.
ACCESSION   DAZFXT010000462 DAZFXT010000000
VERSION     DAZFXT010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 441)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 441)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..441
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.465"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>441
                     /locus_tag="ACHKW0_005413"
     CDS             <1..>441
                     /locus_tag="ACHKW0_005413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478020.1"
                     /translation="EKAEDSFSRLLKQQKEQLALAGQNTELAKLKYQTAQGELKTLTE
                     MQKQELLRNAALIDQQKIREQLRSREETLKNDNVAARASNEAELLGYGQGERARERMR
                     ELQQIRDSFRQKDADLQSQYQTGDISEDFYRQARAQNAQYLSERL"
BASE COUNT          130 a          105 c          140 g           66 t
ORIGIN      
        1 gaaaaagcgg aagacagttt ttcccggcta ctgaagcagc agaaagagca actggcactg
       61 gcgggtcaga atacagagct ggcgaagctg aagtaccaga ctgcgcaggg cgaactgaaa
      121 accctgacgg agatgcagaa gcaggaactg ctgcgtaacg cggccctgat tgaccagcaa
      181 aaaatccggg aacagttgcg atcccgggaa gagaccctga agaatgataa tgtggctgcg
      241 cgtgcatcaa atgaagccga actgctggga tacgggcagg gagaacgagc cagggaacgc
      301 atgcgggagt tgcagcagat ccgcgacagc ttccgccaga aggatgcgga ccttcagtct
      361 cagtatcaga ccggggatat cagtgaggat ttttacagac aggcacgggc acagaacgcg
      421 cagtatctga gcgaacgcct t
//
LOCUS       DAZFXT010000463          440 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.466, whole genome shotgun sequence.
ACCESSION   DAZFXT010000463 DAZFXT010000000
VERSION     DAZFXT010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..440
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.466"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            55..440
                     /locus_tag="ACHKW0_005414"
                     /pseudo
     CDS             55..440
                     /locus_tag="ACHKW0_005414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612174.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          120 a          101 c          119 g          100 t
ORIGIN      
        1 cttatttaag tgatagtctt aatactagtt tttagactag tcattggaga gcagatgatt
       61 gatgtcttag gaccggagaa acgcagacgg cgtaccacac aggaaaagat cgcaattgtt
      121 cagcagagct ttgaaccagg gatgacggtc tccctcgttg cccggcaaca tggtgtagca
      181 gccagccagt tatttctctg gcgtaagcaa taccaggaag gaagtcttac tgctgtcgcc
      241 gccggagaac aggttgttcc tgcctctgaa cttgctgccg ccatgaagca gattaaagaa
      301 ctccagcgcc tgctcggcaa gaaaacgatg gaaaatgaac tcctcaaaga agccgttgaa
      361 tatggacggg caaaaaagtg gatagcgcac gcgcccttat tgcccgggga tggggagtaa
      421 gcttagtcag ccgttgtctc
//
LOCUS       DAZFXT010000464          438 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.467, whole genome shotgun sequence.
ACCESSION   DAZFXT010000464 DAZFXT010000000
VERSION     DAZFXT010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.467"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>438
                     /locus_tag="ACHKW0_005415"
     CDS             <1..>438
                     /locus_tag="ACHKW0_005415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HFO7478021.1"
                     /translation="LNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTVSDVMSVLHDSYDNGKSGEKFLQE
                     LCSVDLLVLDEIGVQRETKNEQVVLHQIIDRRTASLCSVGMLT"
BASE COUNT          118 a           98 c          129 g           93 t
ORIGIN      
        1 cctgaatcgt tcgggcatcc agcctctgca cagcaaatgc tcgtttgcga attatcaggt
       61 gcagaacgac gggcaaaaat acgcgctgag ccaggccaaa tccatagctg acgaactgat
      121 gaccgggtgc acgaattttg tgttcagcgg taaaaccggc accgggaaaa atcaccttgc
      181 agcggcgatg ggtaaccggc tgatggcgaa ggggcgcagc gtgattatcg tcaccgtgtc
      241 tgatgtcatg agcgtgttgc atgacagcta cgacaacggc aaatccgggg aaaaattttt
      301 acaggagctt tgcagtgttg atttgctggt cctggatgaa ataggcgttc agcgggagac
      361 gaaaaacgag caggtggtat tacaccagat aattgatcgc cggacagcat cactgtgcag
      421 tgtcgggatg ttaacaaa
//
LOCUS       DAZFXT010000465          437 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.468, whole genome shotgun sequence.
ACCESSION   DAZFXT010000465 DAZFXT010000000
VERSION     DAZFXT010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.468"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..93)
                     /gene="yrhD"
                     /locus_tag="ACHKW0_005416"
     CDS             complement(<1..93)
                     /gene="yrhD"
                     /locus_tag="ACHKW0_005416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrhD"
                     /protein_id="HFO7478022.1"
                     /translation="MEKLTTELHSLSEMDRRHVVSILAEIANGYD"
     gene            complement(188..>437)
                     /gene="yrhA"
                     /locus_tag="ACHKW0_005417"
     CDS             complement(188..>437)
                     /gene="yrhA"
                     /locus_tag="ACHKW0_005417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein YrhA"
                     /protein_id="HFO7478023.1"
                     /translation="LSIPEPSVKNLFAVNEFYRNNDDFINPDLQERLVIGDDSISIFT
                     YDIKSNFFEIRDNIGTENIFSSFSDFSSFLNEIMDSCS"
BASE COUNT          138 a           71 c           72 g          156 t
ORIGIN      
        1 atcatagcca ttagctattt cggctaaaat agagactaca tgtcttcggt ccatctcact
       61 taaggagtgt agttccgttg taagtttttc catagcttgc actgctaaat ttcgaacaag
      121 gaattttctg ctggtaatct ctaaaaagat ggcatggttt acaatgattt ttgtttcctt
      181 ttgattatta tgaacaactg tccatgattt cgtttaagaa tgaagagaaa tcactaaacg
      241 aactgaatat attttctgtg ccaatattat ctctaatttc aaaaaagtta cttttaatgt
      301 cataagtaaa tattgaaatg ctatcatccc cgatcactaa ccgttcttgt agatcagggt
      361 ttatgaaatc atcattattt ctataaaatt catttacggc aaaaaggttt ttaactgaag
      421 gttctggaat cgagagg
//
LOCUS       DAZFXT010000466          434 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.469, whole genome shotgun sequence.
ACCESSION   DAZFXT010000466 DAZFXT010000000
VERSION     DAZFXT010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.469"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            183..>434
                     /locus_tag="ACHKW0_005418"
     CDS             183..>434
                     /locus_tag="ACHKW0_005418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HFO7478024.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVIN"
BASE COUNT          107 a          106 c          107 g          114 t
ORIGIN      
        1 cctccagtgc agagaaaatc ggccagtttt ctctgcctgc agtccgcatg ccgtatcggg
       61 ccttgggttc taacctgttg cgtagattta tgcagcggac tgcctttctc ccaaagtgat
      121 aaaccggaca gtatcatgga ccggttttcc cggtaatccg tatttgcaag gttggtttca
      181 ctatggaaca tgaacttcat tatatcggta tcgacaccgc taaagagaaa ctggatgtcg
      241 atgtgttgcg tcctgatggt cgtcatcgca ccaaaaaatt cgctaacacc actaaagggc
      301 acgatgagct ggtgagctgg ctgaaaggtc acaagattga ccatgcgcat atctgcatcg
      361 aagcgaccgg cacctatatg gaacctgtcg ctgagtgcct ttacgatgct ggctacatag
      421 tgtcagtcat taat
//
LOCUS       DAZFXT010000467          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.470, whole genome shotgun sequence.
ACCESSION   DAZFXT010000467 DAZFXT010000000
VERSION     DAZFXT010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.470"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..75
                     /locus_tag="ACHKW0_005419"
     CDS             <1..75
                     /locus_tag="ACHKW0_005419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478025.1"
                     /translation="EGIRITDIDTSGIFDSDDMTIKAA"
     gene            148..312
                     /locus_tag="ACHKW0_005420"
     CDS             148..312
                     /locus_tag="ACHKW0_005420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease FtsH-inhibitory lysogeny factor CIII"
                     /protein_id="HFO7478026.1"
                     /translation="MQYAIAGWPVAGCPSESLLERITRKLRDGWKRLIDILNQPGVPK
                     NGSNNYGYPD"
     gene            281..424
                     /gene="kil"
                     /locus_tag="ACHKW0_005421"
     CDS             281..424
                     /gene="kil"
                     /locus_tag="ACHKW0_005421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HFO7478027.1"
                     /translation="MDQTIMAIQTKFTIATFIGDEKMFREAVDAYKKWILILKLRSSK
                     SIH"
BASE COUNT          136 a           92 c           87 g          110 t
ORIGIN      
        1 gaaggaataa gaattaccga tatcgataca tcaggaatat ttgattcaga tgatatgact
       61 atcaaggccg cctgagcgcg gcgttaccgc ataccaatta cgcttcactc gaggcgtttt
      121 tcgttatgta taaataagga gcacaccatg caatatgcca ttgcagggtg gcctgttgct
      181 ggctgccctt ccgaatcttt acttgaacga atcacccgta aattacgtga cggatggaaa
      241 cgccttatcg acatacttaa tcagccagga gtcccaaaaa atggatcaaa caattatggc
      301 tatccagact aaattcacta tcgccacttt tattggcgat gaaaagatgt ttcgtgaggc
      361 cgtcgacgct tataaaaaat ggatattaat actgaaactg agatcaagca aaagcattca
      421 ctaac
//
LOCUS       DAZFXT010000468          425 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.471, whole genome shotgun sequence.
ACCESSION   DAZFXT010000468 DAZFXT010000000
VERSION     DAZFXT010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.471"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..389
                     /gene="espX1"
                     /locus_tag="ACHKW0_005422"
     CDS             <1..389
                     /gene="espX1"
                     /locus_tag="ACHKW0_005422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HFO7478028.1"
                     /translation="SNGEINNLFIKKSNIFGASFTNTRIKNLLCVDIMPGRWTTQLVN
                     KHLGYRYTGVFKTLASIDDKPSRFEILIPLVQTLVRDNVKLNNDVYKELNKFMHDYDK
                     TSSEMRKYLKSINECMFLMKNIAHQN"
BASE COUNT          172 a           70 c           64 g          119 t
ORIGIN      
        1 tttcaaatgg agaaattaat aatctcttca ttaaaaaaag caacatattt ggagcatcat
       61 tcactaatac cagaataaaa aatttgctct gtgtagatat tatgcctggt cgatggacca
      121 ctcaacttgt caataagcac ttaggttaca gatacacagg agtattcaaa accctggcaa
      181 gtattgatga caaaccgtcc cgtttcgaaa ttttgatacc attggtacaa actttagttc
      241 gcgacaatgt aaagctaaat aatgatgtgt ataaagaact taataagttt atgcacgatt
      301 acgacaaaac atcatcagaa atgagaaaat accttaaaag catcaacgag tgtatgttcc
      361 ttatgaaaaa catagctcat caaaattaaa aaatctcaaa aaagaagtaa tttcagaatg
      421 aaatt
//
LOCUS       DAZFXT010000469          421 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.472, whole genome shotgun sequence.
ACCESSION   DAZFXT010000469 DAZFXT010000000
VERSION     DAZFXT010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..421
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.472"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>421
                     /gene="rfaY"
                     /locus_tag="ACHKW0_005423"
     CDS             <1..>421
                     /gene="rfaY"
                     /locus_tag="ACHKW0_005423"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HFO7478029.1"
                     /translation="VLKVYAPKHKMTERFLKSCIKKDYYEDLIYQTDRVRGEGIQSIN
                     DYFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSG
                     DPHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLER"
BASE COUNT          151 a           59 c           79 g          132 t
ORIGIN      
        1 ttgtacttaa agtttatgcg ccaaagcata aaatgaccga acgtttctta aaatcttgca
       61 taaaaaaaga ttattatgaa gatttaattt atcaaaccga tcgggtacgt ggtgaaggca
      121 tccaatcaat taatgactat tttttgctag ctgaacgtaa aacattaaat ttcgctcatt
      181 attatattat gctcattgag tatattgaag gtgtcgggtt aaatgagtat ttggaaattt
      241 ctgaagatct gaaggatcaa ttatcagaat cgataaaaga gttgcatcag catggaatgg
      301 tgtctggcga tcctcataaa ggaaatttta tagtatccga aaaaggttta agactaattg
      361 acctttcagg taaaaaaaca accgctgttt taaaagcaaa agatcgcatt gatcttgaac
      421 g
//
LOCUS       DAZFXT010000470          416 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.473, whole genome shotgun sequence.
ACCESSION   DAZFXT010000470 DAZFXT010000000
VERSION     DAZFXT010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..416
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.473"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..271
                     /locus_tag="ACHKW0_005424"
     CDS             <1..271
                     /locus_tag="ACHKW0_005424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HFO7478030.1"
                     /translation="TLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYT
                     QRIERHNLNLRQHLARPGRKSLSFSKSVELHDKDIGHYLNIKHYQ"
BASE COUNT          104 a           90 c          109 g          113 t
ORIGIN      
        1 cactctggcc acactggagc gtcttctgag cctgctgtcg gcctttgagg tcgtggtatg
       61 gatgacggat ggctggccgc tgtatgaatc acgcctgaag ggaaagctgc acgttatcag
      121 caagcgttac actcagcgca ttgagcgaca taatctgaat ctgagacaac acctggcaag
      181 gccgggacgg aagtcactgt cgttctcaaa atcggtggag ctgcatgaca aggacatcgg
      241 gcattatctg aacataaaac actatcagta agttggagtc attacctaaa ggcctctcta
      301 cactgtgctt gtatggggct tccctgatca tggcggttat ctatgtaata gcatatgtac
      361 ttacgtggtt tctgaggtgg tagcgcgtaa tattatttta tgtaaaaata tcttgt
//
LOCUS       DAZFXT010000471          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.474, whole genome shotgun sequence.
ACCESSION   DAZFXT010000471 DAZFXT010000000
VERSION     DAZFXT010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.474"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..370)
                     /locus_tag="ACHKW0_005425"
     CDS             complement(11..370)
                     /locus_tag="ACHKW0_005425"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000024298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - ATP-dependent diacylglycerol
                     kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HFO7478031.1"
                     /translation="MANTTTGVTRIIKAAGYSWKGLCAAWRYEAAFRQECILVLSALI
                     ISFWLDVDSVTRVLLIGSAMMVIIVEIINSAIEAVVDRIGSEHHELSGRAKDMGSAAV
                     LITILVAIIIWGVLLLS"
BASE COUNT          135 a           93 c           65 g          119 t
ORIGIN      
        1 atgttgggcg ttatgaaagt aataaaactc cccatataat gatagcaact aaaatcgtaa
       61 tcaacactgc ggctgaaccc atatcttttg ctctgcctga taattcgtga tgttctgaac
      121 caattctgtc aacaactgct tcaattgcac tgttaattat ttcaacaata attaccatca
      181 ttgccgaacc aatgagtaat actcgagtaa cagagtcaac atccagccaa aaggatatta
      241 tcagtgctga cagaaccaga atgcactcct gacgaaaggc tgcttcatag cgccatgccg
      301 cacataagcc tttccatgaa taacccgctg ctttaataat tctggtaaca ccagtggtcg
      361 tattagccat aaatacctct tatttaatta tatgtctgaa tatgcattat aa
//
LOCUS       DAZFXT010000472          412 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.475, whole genome shotgun sequence.
ACCESSION   DAZFXT010000472 DAZFXT010000000
VERSION     DAZFXT010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.475"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>412
                     /locus_tag="ACHKW0_005426"
     CDS             <1..>412
                     /locus_tag="ACHKW0_005426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7478032.1"
                     /translation="VANIPVPADGKSITPDDVRPMLEQMVKEAVSHIPAPRDGRDYDP
                     EVLKQAVLEAVNALPAPQDGRDATALEVLPAIDDQKSFPRGTYATHLGGLWRAYEKTH
                     GMRGWECLVDGVADIDVSMTDERLFSVVIRQSSGQ"
BASE COUNT           85 a          103 c          142 g           82 t
ORIGIN      
        1 ggttgcgaat attccggtac cggcggacgg caaaagtatc acccccgatg atgtgcgtcc
       61 gatgcttgag cagatggtga aagaggccgt aagccatatc cctgctccgc gtgatggtcg
      121 tgattacgat ccggaagtac tgaagcaggc ggttctggag gcggtgaatg cccttccggc
      181 tccgcaggac gggcgtgatg ccacggcact ggaagtgctc cccgctattg acgatcaaaa
      241 atcctttccc cggggcacgt atgccacaca cctgggtgga ctctggcggg cgtatgaaaa
      301 aacgcacggg atgcggggat gggaatgcct ggttgacggg gtggcggata tcgacgtcag
      361 catgacggat gagcggttgt tctctgtggt gatccggcag agcagtggcc ag
//
LOCUS       DAZFXT010000473          410 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.476, whole genome shotgun sequence.
ACCESSION   DAZFXT010000473 DAZFXT010000000
VERSION     DAZFXT010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.476"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            277..>410
                     /locus_tag="ACHKW0_005427"
     CDS             277..>410
                     /locus_tag="ACHKW0_005427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140911 - pore-forming activity [Evidence
                     IEA];
                     GO_process: GO:0001907 - symbiont-mediated killing of host
                     cell [Evidence IEA];
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HFO7478033.1"
                     /translation="MEKITTGVSYTTSAVGTGYWLLQLLDKVSPSQWVAIGVLGSLLF
                     G"
BASE COUNT           91 a           84 c          117 g          118 t
ORIGIN      
        1 ccactctgaa ggacgctggc taaaacgggt agatgtactt cacatgatac ttatatttgg
       61 cagtacattt tctgacagac agtgacggat gttgtcaaga tattgtgtca tttataacct
      121 gaatcagggg ttggtcggaa tgttatctgg catttttagc agagcctgaa tgccataatc
      181 acggctcccg gcgttggccg tcagtgggtg acactggcgg ctttttgttt tcctttactt
      241 tcattttctg tcggcggtga cggagacata catcggatgg aaaaaatcac aacaggtgtg
      301 tcatacacca cgtcagcggt ggggacggga tactggttac tgcagctgct ggacaaagtc
      361 tctccgtccc agtgggtggc aataggtgtg ctggggagtc tgctgtttgg
//
LOCUS       DAZFXT010000474          409 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.477, whole genome shotgun sequence.
ACCESSION   DAZFXT010000474 DAZFXT010000000
VERSION     DAZFXT010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..409
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.477"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>409)
                     /locus_tag="ACHKW0_005428"
     CDS             complement(<1..>409)
                     /locus_tag="ACHKW0_005428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HFO7478034.1"
                     /translation="TPFPFENNNSHTGVVTEPILGKLIGQGSTAEIFEDMNDSSALYK
                     KYDLVGNQHNEVLEMARQESALFNTFYGDDASVVIQYGGDVYLRMLRVPGIPLSDIDT
                     ADIPDNLESLYLQLICKLNELSIIHYDLNTGNM"
BASE COUNT          133 a           86 c           68 g          122 t
ORIGIN      
        1 aacatattac ctgtattaag atcgtaatgg attatactca actcattcaa tttacatatc
       61 aactgcagat aaaggctctc tagattatca ggaatatcag ctgtatcaat atcactaagg
      121 ggaatccctg gcacgcgtag cattcggaga tacacatcac cgccatactg tataacaact
      181 gatgcgtcat cgccataaaa agtattaaaa agggcagatt cttgcctagc catttccaga
      241 acttcattgt gctggttacc aacaagatca tactttttat ataaagcaga cgaatcattc
      301 atatcttcaa agatttctgc tgttgaacct tgccctatta acttaccgag aataggctcc
      361 gttactacgc cagtatgaga attattattc tcaaatggga aaggtgttt
//
LOCUS       DAZFXT010000475          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.478, whole genome shotgun sequence.
ACCESSION   DAZFXT010000475 DAZFXT010000000
VERSION     DAZFXT010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.478"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>401)
                     /locus_tag="ACHKW0_005429"
     CDS             complement(<1..>401)
                     /locus_tag="ACHKW0_005429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YPO3983 family protein"
                     /protein_id="HFO7478035.1"
                     /translation="NNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLGL
                     SDISAKVDPYRLIKYHFPGPGSINVAFSASSSGTKISQRECTDILFAEMKELAKMFSF
                     FGQNKTLIEDLIEHFRYGNGSNFHSQQLNLS"
BASE COUNT          137 a           59 c           73 g          132 t
ORIGIN      
        1 aaagataaat ttaattgttg gctatgaaaa ttgctgccat ttccatatct aaaatgttca
       61 atcagatctt cgataagtgt tttattttgt ccaaaaaagg aaaacatttt tgctagttct
      121 ttcatttctg caaataaaat atcagtgcat tcacgttgtg atatttttgt tccggaactt
      181 gaggcactaa aagcaacatt aatagaacct gggccaggga aatggtattt aataagtctg
      241 taaggatcaa cttttgctga gatatcgctt aggccgagtg agagaaagtc ttgttctacc
      301 atatcaccat attgcatatc atcagcagaa aaatcatcga ataaactaat agtttcgaaa
      361 agtgtacagg gaatattaat tggcggaagt aatatgttat t
//
LOCUS       DAZFXT010000476          401 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.479, whole genome shotgun sequence.
ACCESSION   DAZFXT010000476 DAZFXT010000000
VERSION     DAZFXT010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.479"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          139 a           83 c           73 g          106 t
ORIGIN      
        1 aataagtgat attggttgtc tggagattca gggggccagt ctagtctgtc atgaaacagt
       61 tttatgacaa gacatcaaaa aaatgtcaaa aacgtgatct ttcttcagct tttcagaata
      121 ttgagtacca ggaagtcaat atcaattctg ggacacatat gcatttacca ttaaaaccgg
      181 gcacaacaag ctgttcagtc tcaatccata ccccgtgttt tatcaatatc agaataagaa
      241 tatctgcgga taaataaaaa aggaatgtcg agggagctta ccgcagaggg ctctccacct
      301 cacaaaccat ttacaaaaat gataccggca tatctttggt ccctgtcaca accagacatt
      361 gacaatccaa tataacccca aatatatttc gggtaagaag t
//
LOCUS       DAZFXT010000477          398 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.480, whole genome shotgun sequence.
ACCESSION   DAZFXT010000477 DAZFXT010000000
VERSION     DAZFXT010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..398
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.480"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..360
                     /locus_tag="ACHKW0_005430"
     CDS             <1..360
                     /locus_tag="ACHKW0_005430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HFO7478036.1"
                     /translation="YHLAGIDMPDFEREDDWWRNGQNLYLDNMAVTGFYRVPLSSAQA
                     GDILLCCFGASVANHAAIYCGNGELLHHLPEQLSKRERYSEKWQRRTHSVWRHRHWHA
                     SAFTGICNDLAAASACM"
     gene            345..398
                     /locus_tag="ACHKW0_005431"
                     /pseudo
     CDS             345..398
                     /locus_tag="ACHKW0_005431"
                     /inference="COORDINATES: protein motif:HMM:NF018510.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           82 a          112 c          116 g           88 t
ORIGIN      
        1 taccatctgg cgggaattga tatgccggat tttgagcgtg aggatgactg gtggcgcaac
       61 ggtcagaacc tttacctgga caatatggcg gtcacaggct tttaccgggt gcccctgtcc
      121 tctgcacagg cgggcgatat cctgctgtgc tgctttggcg catcggtggc caatcatgcc
      181 gccatttact gcggcaacgg tgaactgctt caccatctgc ctgaacaact gagtaaacgg
      241 gagaggtatt ccgaaaaatg gcaacgacga acgcattccg tctggcgtca ccgccactgg
      301 cacgcatctg ccttcacggg gatttgcaac gatttggccg ccgcctcagc ctgtatgtga
      361 acacggcagc ggaagccatc cgggcgctgt cgttacag
//
LOCUS       DAZFXT010000478          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.481, whole genome shotgun sequence.
ACCESSION   DAZFXT010000478 DAZFXT010000000
VERSION     DAZFXT010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.481"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..302
                     /locus_tag="ACHKW0_005432"
     CDS             <1..302
                     /locus_tag="ACHKW0_005432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO7478037.1"
                     /translation="LLDILRHKALTQMAQESGGSATVRLNTLDWLGGQGREQADNEWH
                     DAINWLGDWCSEEQHPVIWSTTQAAEHLPVRMPRLCSAERLSESMVDEIFQKGAA"
     gene            299..>397
                     /locus_tag="ACHKW0_005433"
     CDS             299..>397
                     /locus_tag="ACHKW0_005433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478038.1"
                     /translation="MSTEMKTGLVLSGGGAVGAYQAGVVKALAECGT"
BASE COUNT           94 a           86 c          137 g           80 t
ORIGIN      
        1 agctactgga tattctcaga cataaagcat taacgcagat ggcacaggag agtggcggta
       61 gtgcaaccgt gcgtctgaac actctggact ggcttggtgg tcagggccgg gagcaggcag
      121 ataacgagtg gcatgacgcg attaactggc tgggagactg gtgcagtgaa gagcagcatc
      181 cggtgatctg gtcaaccacg caggctgcag aacacttacc ggttcgcatg cctcgcctct
      241 gctctgcaga acgcctctct gaaagtatgg tcgatgaaat atttcagaag ggggctgcat
      301 gagtacagaa atgaaaacgg ggctggtgct gtccggtggc ggggcggtgg gcgcttatca
      361 ggcgggagtg gttaaggcac tggcagagtg tggtaca
//
LOCUS       DAZFXT010000479          397 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.482, whole genome shotgun sequence.
ACCESSION   DAZFXT010000479 DAZFXT010000000
VERSION     DAZFXT010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.482"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..170)
                     /locus_tag="ACHKW0_005434"
     CDS             complement(<1..170)
                     /locus_tag="ACHKW0_005434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478039.1"
                     /translation="MAHDTKLYNSDDSAVFASRRGRCFHAFKSDWYQHPPCTEEQAEW
                     LIQCYRRRGCEV"
     gene            complement(338..>397)
                     /locus_tag="ACHKW0_005435"
     CDS             complement(338..>397)
                     /locus_tag="ACHKW0_005435"
                     /inference="COORDINATES: protein motif:HMM:NF013968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HFO7478040.1"
                     /translation="RVRTGQTEVAVFTAYEPEE"
BASE COUNT           86 a          102 c          117 g           92 t
ORIGIN      
        1 ttaacctcgc atccgcgcct gcggtaacac tgaatgagcc attcggcctg ttcttcagtg
       61 catgggggat gctggtacca gtcagatttg aatgcgtgaa aacaccgtcc gcgcctgctg
      121 gcaaagacgg cagaatcatc agaattgtat aatttggtat cgtgcgccat cggttgtctc
      181 tgctggcgca gcaggtgcca gttgttcagg ctggcgtgcg aattgtaaac cagaatgcca
      241 ggaaaaaaca aaacccgccg aagcgggtta agtgcgggtg cgttgaggat gcctgattca
      301 tcagaggtgg cgagggattt ctcccccgcc aggtctctta ctcctcaggt tcgtaagctg
      361 tgaagacagc gacctccgtc tggccggttc ggactcg
//
LOCUS       DAZFXT010000480          395 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.483, whole genome shotgun sequence.
ACCESSION   DAZFXT010000480 DAZFXT010000000
VERSION     DAZFXT010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.483"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..206)
                     /gene="ydfK"
                     /locus_tag="ACHKW0_005436"
     CDS             complement(<1..206)
                     /gene="ydfK"
                     /locus_tag="ACHKW0_005436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein YdfK"
                     /protein_id="HFO7478041.1"
                     /translation="MKSKDTLKWFPAQLPEVRIILGDAVVEVAKQGRPINTRTLLDYI
                     EGNIKKKSWLDNKELLQTAISVLK"
     gene            complement(275..388)
                     /locus_tag="ACHKW0_005437"
     CDS             complement(275..388)
                     /locus_tag="ACHKW0_005437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnaM/YnfT family protein"
                     /protein_id="HFO7478042.1"
                     /translation="MNSILIITSLLIIFSIFSHALIKLGIGISNNPDKTDV"
BASE COUNT          102 a           67 c           72 g          154 t
ORIGIN      
        1 tctttaagaa ctgatatcgc tgtttgtaat aattctttgt tatccagcca tgactttttc
       61 tttatgtttc cttcaatgta atcaagcaat gttctggtat tgataggtct tccctgtttt
      121 gctacttcca ctacagcatc ccctaggata attcttactt caggaagctg cgcagggaac
      181 cactttaggg tgtcttttga tttcatgaag atattcctta aaatattatt gattttcatt
      241 gcgatattgt atgtctgatt caggatatgt tgacttatac atcggttttg tctgggttat
      301 tggatatgcc aatccctaat tttattagag catgactaaa aatgctgaat atgataagga
      361 gcgaagtgat tatcagtatg ctgttcatat agcct
//
LOCUS       DAZFXT010000481          391 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.484, whole genome shotgun sequence.
ACCESSION   DAZFXT010000481 DAZFXT010000000
VERSION     DAZFXT010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.484"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          115 a           70 c           99 g          107 t
ORIGIN      
        1 tgctggagat aaatgcaccg gcagcataat aaaaaaagag ccagcgactg acctgaaaga
       61 agacgctggc taaaaggcct tatatgtttg tagagactta tttttcacag acagcaatga
      121 tgcctgtcaa tatattatca atatgcggat tgtttcagtt acagatgctt tattaaggaa
      181 aaaaacagcc agcactgact ttcggtggag aggtgctggc tcagaaggat agttggattt
      241 cacatgatac ttatgcctgg cggtatattt tctgacagac agtgacgggt gttgtcaaga
      301 tattgtgtca tttataacct gaatcagggg ggggccggaa tgttatctgg catttttagc
      361 agagcctgaa tgccataatc acggctcccg g
//
LOCUS       DAZFXT010000482          387 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.485, whole genome shotgun sequence.
ACCESSION   DAZFXT010000482 DAZFXT010000000
VERSION     DAZFXT010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..387
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.485"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..303
                     /locus_tag="ACHKW0_005438"
     CDS             <1..303
                     /locus_tag="ACHKW0_005438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HFO7478043.1"
                     /translation="SSNDNDSVDLRNIKCPEGSERVGDYHTHGFYSDDKGNKVTKEND
                     VYDSLNFSSKDLTNSYMNGMEKKEYSSYLGTPNNTYLKYNPKAKGNGVTIIRQGSN"
     gene            305..>387
                     /locus_tag="ACHKW0_005439"
     CDS             305..>387
                     /locus_tag="ACHKW0_005439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein dsORF-f3"
                     /protein_id="HFO7478044.1"
                     /translation="MKAIQCCYFLALVSSHLMAGVMGDDNI"
BASE COUNT          151 a           48 c           76 g          112 t
ORIGIN      
        1 agttcaaatg ataatgatag tgtagacttg cgaaatataa aatgccctga aggttcagag
       61 agagtaggcg attatcacac tcacggtttt tactctgacg ataaaggaaa taaagtaaca
      121 aaagaaaatg atgtttatga tagtctaaat ttttcaagca aagatttaac gaattcttat
      181 atgaatggaa tggaaaaaaa agaatacagt agttacttgg gaacaccaaa taacacctat
      241 ctaaaatata atcccaaagc taaagggaat ggagttacaa ttatcaggca agggagtaat
      301 taaaatgaag gcaatacaat gttgttattt ccttgcttta gtctccagtc atttaatggc
      361 tggagttatg ggtgatgata atatatt
//
LOCUS       DAZFXT010000483          386 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.486, whole genome shotgun sequence.
ACCESSION   DAZFXT010000483 DAZFXT010000000
VERSION     DAZFXT010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.486"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..337)
                     /locus_tag="ACHKW0_005440"
     CDS             complement(<1..337)
                     /locus_tag="ACHKW0_005440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7478045.1"
                     /translation="MAEIKTLHLVPREGMQVSEKPSVVRVRFGDGYEQRRPTGLNPQL
                     KTFQAVFRVTDESTRRWLEEFLSWHGGYRAFLWRPPKHNRTVRVVCREWSVTDNARYS
                     DFSCTIEQVV"
BASE COUNT           86 a          144 c           79 g           77 t
ORIGIN      
        1 tcaccacctg ctctatcgtg caactgaaat cactgtaccg ggcattatcc gtgacactcc
       61 actcacggca cacaaccctc accgtccggt tatgtttcgg cggtcgccac aaaaaggcac
      121 ggtaaccacc atgccacgat aaaaactctt ccagccagcg ccgggttgac tcatccgtca
      181 cccggaacac cgcctgaaac gtcttcagtt gaggattcag ccctgtgggg cggcgctgtt
      241 cataaccgtc accaaaccgc accctcacca ccgacggctt ctcactcacc tgcatccctt
      301 cacgcgggac cagatgcagc gtttttatct cagccactca gcattcctcc gtcacgtcgc
      361 atggacagca tcaccgcctg cacccg
//
LOCUS       DAZFXT010000484          377 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.487, whole genome shotgun sequence.
ACCESSION   DAZFXT010000484 DAZFXT010000000
VERSION     DAZFXT010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.487"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            44..>377
                     /locus_tag="ACHKW0_005441"
     rRNA            44..>377
                     /locus_tag="ACHKW0_005441"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          111 a           80 c          115 g           71 t
ORIGIN      
        1 cgcaacacga tgatgaatcg aaagaaacat cttcgggttg tgaggttaag cgactaagcg
       61 tacacggtgg atgccctggc agtcagaggc gatgaaggac gtgctaatct gcgataagcg
      121 tcggtaaggt gatatgaacc gttataaccg gcgatttccg aatggggaaa cccagtgtga
      181 ttcgtcacac tatcattaac tgaatccata ggttaatgag gcgaaccggg ggaactgaaa
      241 catctaagta ccccgaggaa aagaaatcaa ccgagattcc cccagtagcg gcgagcgaac
      301 ggggagcagc ccagagcctg aatcagtgtg tgtgttagtg gaagcgtctg gaaaggcgcg
      361 cgatacaggg tgacagc
//
LOCUS       DAZFXT010000485          374 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.488, whole genome shotgun sequence.
ACCESSION   DAZFXT010000485 DAZFXT010000000
VERSION     DAZFXT010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.488"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            158..>374
                     /locus_tag="ACHKW0_005442"
     CDS             158..>374
                     /locus_tag="ACHKW0_005442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HFO7478046.1"
                     /translation="MSSKNRTRRTTTRNIRFPNQIIEQINIALDLKGSGNFSAWVIEA
                     CRRRLINEKYSQFVPNKDKHDQSTCSDR"
BASE COUNT          143 a           83 c           60 g           88 t
ORIGIN      
        1 taattatcat ctgcatgtca caacgtgcat ctacgccatc aaaccacgag aaataatcaa
       61 ttattacgca ggtatcgtat taattgatct gcatcaaatt agcgtaaaag caacttcaga
      121 taatacaaat cagcaacact gaatacgggg caacattatg tcatcaaaga acagaacccg
      181 cagaacaaca acccgcaaca tccgatttcc aaaccagata attgaacaaa ttaacatcgc
      241 tcttgacctg aaaggttcag gtaatttttc agcgtgggtt attgaagcct gcagaagaag
      301 attaattaat gaaaaatatt ctcaatttgt acccaacaaa gacaaacacg accagagcac
      361 ctgttcagac aggt
//
LOCUS       DAZFXT010000486          372 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.489, whole genome shotgun sequence.
ACCESSION   DAZFXT010000486 DAZFXT010000000
VERSION     DAZFXT010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..372
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.489"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>372
                     /locus_tag="ACHKW0_005443"
     CDS             <1..>372
                     /locus_tag="ACHKW0_005443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7478047.1"
                     /translation="TPDDVRPMLEQMVKEAVSHIPAPRDGRDYDPDVLRKAVLEAVNA
                     LPAPQDGRDATALEVLPAIDDQKSFPRGTYATHLGGFWRAYEKTHGMRGWECLVDGVA
                     DIDVSMTGERLFSVVIRHSSGQ"
BASE COUNT           71 a           91 c          132 g           78 t
ORIGIN      
        1 acccccgatg atgtgcgtcc gatgcttgag cagatggtga aagaggctgt aagccatatc
       61 cctgctccgc gtgatggtcg tgattacgat ccggatgttc tgcggaaggc ggttctggag
      121 gcggtgaatg ccctgccggc tccgcaggac gggcgtgatg ccacggctct ggaagtgctc
      181 cccgctattg acgatcaaaa atcctttccc cggggcacgt atgccacaca cctgggagga
      241 ttctggcggg cgtatgaaaa aacgcacggg atgcggggat gggaatgcct ggttgacggg
      301 gtggcggata ttgacgtcag tatgacgggg gagcggttgt tctctgtggt gatccggcac
      361 agcagtggcc ag
//
LOCUS       DAZFXT010000487          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.490, whole genome shotgun sequence.
ACCESSION   DAZFXT010000487 DAZFXT010000000
VERSION     DAZFXT010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.490"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>371
                     /locus_tag="ACHKW0_005444"
     CDS             <1..>371
                     /locus_tag="ACHKW0_005444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO7478048.1"
                     /translation="LRGKKVRTTVSRKAVAACDRVNRQFVAERPDQLWVADFTWVSTW
                     QGFVYVAFIIDVFAGYIVGWQVSSSMETTFVLDALEQALWARRPSGTIHHSDKGSQYV
                     SLAYTQRLKEAGLLASTGSTG"
BASE COUNT           81 a           94 c          113 g           83 t
ORIGIN      
        1 ctccggggta aaaaggtccg cactaccgtc agccggaaag ccgttgccgc atgcgaccgc
       61 gtaaaccgtc agttcgtggc agaacgtcca gaccagttgt gggtggctga ttttacctgg
      121 gtaagcacat ggcagggctt cgtttatgtg gcgttcatca ttgatgtgtt cgccggatac
      181 atcgtgggat ggcaggtctc atcatccatg gaaacaacat tcgtgctgga tgcactggag
      241 caggcgttgt gggcccgtcg gccgtccggc acaatccatc acagtgataa aggttctcag
      301 tatgtatcgc tggcctacac gcagcggctt aaggaagccg gattactggc atcaacaggg
      361 agtactggcg a
//
LOCUS       DAZFXT010000488          371 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.491, whole genome shotgun sequence.
ACCESSION   DAZFXT010000488 DAZFXT010000000
VERSION     DAZFXT010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.491"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>371)
                     /locus_tag="ACHKW0_005445"
     CDS             complement(<1..>371)
                     /locus_tag="ACHKW0_005445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7478049.1"
                     /translation="WRGFTRSVLSMLTEIFLKQAMVGIVGSIGSAIGGAFGGGASAST
                     GTAIQAAAANFHFATGGFTGTGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGY
                     AEGGYVGGAGSPAQMRRTEGI"
BASE COUNT           82 a          140 c           86 g           63 t
ORIGIN      
        1 taatgccttc cgtccgccgc atctgcgccg gacttccggc accgcccaca taaccacctt
       61 ccgcatagcc ccgcatcagg cggtacaggt tgccgacgcc aatccggctg gttgcttcct
      121 tcgtgaagac aaactccccg cgatgaacaa ttcccgcagg ttcatattta ccccccgtcc
      181 ccgtaaatcc cccggtcgcg aaatggaagt tcgccgccgc agcctgaatg gccgtccccg
      241 tggaggcaga cgcaccacca ccgaaagcac cgccaatggc gctgccgata ctcccgacta
      301 tccccaccat cgcctgcttc agaaaaatct ctgtcagcat ggagagcaca gaacgggtga
      361 aaccacgcca g
//
LOCUS       DAZFXT010000489          369 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.492, whole genome shotgun sequence.
ACCESSION   DAZFXT010000489 DAZFXT010000000
VERSION     DAZFXT010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.492"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>369
                     /locus_tag="ACHKW0_005446"
     CDS             <1..>369
                     /locus_tag="ACHKW0_005446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7478050.1"
                     /translation="AAQNALNSTTAVGSRLMSGALGLVGGVPGLVMLGAAAWYTLYQN
                     QEQARESARQYALTIDEIAHKTPSMSLPEASDNEGRTREALTEQNRLIDEQASRVKSL
                     QEKIAGYQYVLANPGWTTGNG"
BASE COUNT           92 a           94 c          123 g           60 t
ORIGIN      
        1 cgccgcccag aatgcgctga acagtacaac ggcggtgggc tcacgtctga tgagcggtgc
       61 gctgggactg gttggaggcg tacccggact ggtgatgctg ggagctgccg catggtacac
      121 gctgtaccag aatcaggaac aggccaggga gtctgcgcgc cagtatgcac tgacgataga
      181 tgaaatcgcg cataaaacgc catcaatgtc tctgcctgaa gcctcagata acgaagggcg
      241 aacacgggag gcgctgacag agcaaaaccg gctgattgat gagcaggcca gccgggtgaa
      301 atccctgcag gaaaaaatcg ccgggtatca gtatgtgctg gctaatccgg gctggacgac
      361 cggtaacgg
//
LOCUS       DAZFXT010000490          360 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.493, whole genome shotgun sequence.
ACCESSION   DAZFXT010000490 DAZFXT010000000
VERSION     DAZFXT010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.493"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            122..197
                     /locus_tag="ACHKW0_005447"
     tRNA            122..197
                     /locus_tag="ACHKW0_005447"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:156..158,aa:Glu,seq:ttc)
BASE COUNT          102 a           82 c           83 g           93 t
ORIGIN      
        1 taaatagcaa ggcgtttacg cgttgggagt gaggctgaag agaataaggc cgttcgcttt
       61 ctattaatga aagctcaccc tacacgaaaa tatcacgcaa cgcgtgataa gcaattttcg
      121 tgtccccttc gtctagaggc ccaggacacc gccctttcac ggcggtaaca ggggttcgaa
      181 tcccctaggg gacgccactt gctggtttgt gagtgaaagt cgccgacccc aatatctcaa
      241 aacagactgt taagtcttgt ttgagatatt tgctctttaa aaatctggat caagctgaaa
      301 attgaaacac tgaacaacga aagttgttcg tgagtctctc aaattttcgc aactctgaag
//
LOCUS       DAZFXT010000491          353 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.494, whole genome shotgun sequence.
ACCESSION   DAZFXT010000491 DAZFXT010000000
VERSION     DAZFXT010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.494"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>353
                     /locus_tag="ACHKW0_005448"
     CDS             <1..>353
                     /locus_tag="ACHKW0_005448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7478051.1"
                     /translation="ELVEDPEAILRYGRNLLKMDAFGCTSRGQAHRAGLWVIKTGLLE
                     TQTVDFTLGSQGLRHTPGDIIEICDNDYAGTLTGGRVLSIDAASRTLTLDREVTLPET
                     GAATVNLINGSGKPV"
BASE COUNT           75 a          104 c          116 g           58 t
ORIGIN      
        1 gaactggtgg aagacccgga agccatactg cgctacggac gcaacctgct gaagatggac
       61 gcgttcggct gtaccagccg cggtcaggcc caccgtgccg gactgtgggt gataaagacc
      121 ggactgctgg aaacgcagac ggtggatttc acgctcgggt ctcaggggct gcggcacaca
      181 cccggtgaca tcattgaaat ctgtgataac gactatgccg ggaccctgac cggcggacgt
      241 gtcctgtcca ttgatgccgc cagccgcacc ctgacactgg accgtgaggt gaccctgccg
      301 gagacaggtg ccgccacggt gaacctgatt aacggcagcg gtaagccggt gag
//
LOCUS       DAZFXT010000492          351 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.495, whole genome shotgun sequence.
ACCESSION   DAZFXT010000492 DAZFXT010000000
VERSION     DAZFXT010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.495"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            273..351
                     /locus_tag="ACHKW0_005449"
                     /pseudo
     CDS             273..351
                     /locus_tag="ACHKW0_005449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT           81 a          102 c           66 g          102 t
ORIGIN      
        1 aaatgcgaat tatttagtac aaaaagtgaa cttttgatga tctttcccta atctcacata
       61 ggatcagatc tcgtctgatc gttccccctg cgctcactac catggctctt taccacagca
      121 gccggggttg tttgggaccg acttccgaca gcagatcccg gtgggcctgc caccatctct
      181 tcacaagctt atgcttatta catgcaaaac gtcccgtttt gcatctttgt gcctacggca
      241 caccgatgat ctttccctgt gatctttccc tgaatctgcc cctgagaacc tgaatcaaac
      301 gtctgactcg cagaaccatc tgtttctcgc gctctgtaga acttcatgaa a
//
LOCUS       DAZFXT010000493          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.496, whole genome shotgun sequence.
ACCESSION   DAZFXT010000493 DAZFXT010000000
VERSION     DAZFXT010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.496"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>349)
                     /locus_tag="ACHKW0_005450"
     CDS             complement(<1..>349)
                     /locus_tag="ACHKW0_005450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478052.1"
                     /translation="GNTTPLFVAQGNQLFMNDVFLKRLFAASITSSGNPPTFSLTPEG
                     KLTARNADISGAITANTGTLNNVTINENCVIRGKLSANQIEGDLVKTVGKAFPRNNSY
                     ASGTVTVTVYDDQG"
BASE COUNT           67 a          101 c           90 g           91 t
ORIGIN      
        1 ccctggtcat cgtaaactgt gacggttacc gtcccgctgg cataactgtt attccgggga
       61 aaggctttcc ccaccgtctt caccaggtcg ccttcaatct ggtttgcaga cagtttccct
      121 ctgatgacac agttctcgtt aatggtgaca ttattgagcg tgccggtatt cgcggtaatt
      181 gctccgctga tatccgcgtt cctggctgtc agcttccctt ccggcgtcag ggaaaacgtc
      241 ggggggttgc cggatgacgt gatactcgcc gcaaacagcc gcttcaggaa cacatcgttc
      301 atgaacaact gattcccctg cgccacaaat aacggcgtgg tgttgccgt
//
LOCUS       DAZFXT010000494          349 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.497, whole genome shotgun sequence.
ACCESSION   DAZFXT010000494 DAZFXT010000000
VERSION     DAZFXT010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.497"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>349
                     /locus_tag="ACHKW0_005451"
     CDS             <1..>349
                     /locus_tag="ACHKW0_005451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7478053.1"
                     /translation="VIDAIGEGPIEGPVKGLQSILVNKTPLTDTDGNPVIHGVTAVWR
                     AGEQEQTPPEGFESSGAETALGVEVTKAKPVTRTITSANIDRLRVTFGVQSLLETTSK
                     GDRNPSSVRLLIQ"
BASE COUNT           76 a           93 c          114 g           66 t
ORIGIN      
        1 gcgtgattga cgccattggt gaagggccga ttgaaggtcc ggtgaagggg ctgcagagta
       61 ttctggtgaa caaaaccccg ctgacggaca cggacggtaa tcctgtgata catggtgtga
      121 cagcggtctg gcgcgccggg gagcaggagc agacaccacc tgaaggcttt gagtcctccg
      181 gagctgaaac cgcactgggc gtggaagtga cgaaggcaaa gccggtgacg cgcaccatta
      241 cgtccgcgaa cattgaccgc ctgcgggtca ccttcggggt gcagtcactg ttggagacca
      301 cctcaaaggg cgaccgtaat ccctcttctg tccgactgct gattcagtt
//
LOCUS       DAZFXT010000495          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.498, whole genome shotgun sequence.
ACCESSION   DAZFXT010000495 DAZFXT010000000
VERSION     DAZFXT010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.498"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>344
                     /locus_tag="ACHKW0_005452"
     CDS             <1..>344
                     /locus_tag="ACHKW0_005452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HFO7478054.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAZFXT010000496          344 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.499, whole genome shotgun sequence.
ACCESSION   DAZFXT010000496 DAZFXT010000000
VERSION     DAZFXT010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.499"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>344)
                     /locus_tag="ACHKW0_005453"
     CDS             complement(<1..>344)
                     /locus_tag="ACHKW0_005453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HFO7478055.1"
                     /translation="TTISRKAVAAGDRVNRQFVAERPDQLWVADFTYVSTWQGFVYVA
                     FIIDVFAGYIVGWRVSSSMETTFVLDALEQALWARRPSGTVHHSDKGSQYVSLAYTQR
                     LKEAGLLASTGST"
BASE COUNT           78 a          105 c           88 g           73 t
ORIGIN      
        1 gtacttcctg ttgatgccag taatccggct tccttaagcc gctgtgtgta ggccagcgat
       61 acatactgag aacctttatc actgtgatgg accgtgccgg acggtcgacg ggcccataac
      121 gcctgctcca gtgcatccag cacgaatgtc gtctccatgg acgatgagac ccgccacccc
      181 acaatgtatc cggcaaacac atcaatgatg aacgccacat agacgaagcc ctgccatgtg
      241 ctgacgtaag taaaatcagc cacccacagc tggtcaggtc gttctgccac gaactgacgg
      301 tttacgcggt cgcctgcggc aacggctttc cggctgatgg tcgt
//
LOCUS       DAZFXT010000497          337 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.500, whole genome shotgun sequence.
ACCESSION   DAZFXT010000497 DAZFXT010000000
VERSION     DAZFXT010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..337
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.500"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            20..337
                     /locus_tag="ACHKW0_005454"
                     /pseudo
     CDS             20..337
                     /locus_tag="ACHKW0_005454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          129 a           54 c           52 g          102 t
ORIGIN      
        1 aaaatattac aggttattta tgaaagtatt tcacaatata tttaagcact tctcttcaaa
       61 ccatcaagat aagcattcag ataaagttaa cagtcatcaa caccatggca aggttgataa
      121 aaaatatcga tcaaacacag tttaagttaa tatgctgaat gacttttcgc aaatagataa
      181 cgatttaggc ctgaatatca tctatttcct gcaacatggt cactggaggg taaatgaaca
      241 tagtcttcag atggagaaaa tatggtttta caatagtgag ccatctatag atattcaaga
      301 atataatagt ttcgccaaga atactactaa cacgttt
//
LOCUS       DAZFXT010000498          334 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.501, whole genome shotgun sequence.
ACCESSION   DAZFXT010000498 DAZFXT010000000
VERSION     DAZFXT010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..334
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.501"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            20..292
                     /locus_tag="ACHKW0_005455"
     CDS             20..292
                     /locus_tag="ACHKW0_005455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7478056.1"
                     /translation="MSEITSLVTAEAVKEVLRSEEVLSALKQKLRQNLEARLDAEVDA
                     ILDELLGVQAEPPTEAGDTTAESGEVQPESPVADATEPQPESVMML"
BASE COUNT           87 a           86 c           94 g           67 t
ORIGIN      
        1 ctttcgacga gaaaatccca tgtcagaaat tacatccctg gtcactgcag aggcagtgaa
       61 ggaagtcctg cgctctgaag aagtcctgag cgcactgaaa caaaaactcc gccagaatct
      121 tgaggcgcgt cttgatgcag aggttgatgc cattctggat gagctgcttg gtgtacaggc
      181 agagccaccg actgaagcgg gagataccac cgcagagagc ggtgaagttc agcctgaatc
      241 accggtcgcc gatgcgactg aacctcaacc cgaatcggtc atgatgctgt aacggggagt
      301 cagggccatc agtaaacagc tgctggcctt tttc
//
LOCUS       DAZFXT010000499          312 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.502, whole genome shotgun sequence.
ACCESSION   DAZFXT010000499 DAZFXT010000000
VERSION     DAZFXT010000499.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.502"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(207..>312)
                     /locus_tag="ACHKW0_005456"
     CDS             complement(207..>312)
                     /locus_tag="ACHKW0_005456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HFO7478057.1"
                     /translation="IATDEETSLLTVWKRYRVLLNRVDTSVSPDFEGQ"
BASE COUNT          103 a           79 c           44 g           86 t
ORIGIN      
        1 aattccatga acaccccaaa tttacagaag atggtgatag catacatttc aaccttcaaa
       61 atgaatcaat ctttactttc ttaacaaaca tcaccatgac atgacaacaa aaaactggaa
      121 attaactatt gtttttgagt atctggcaac tcattcatcc ttccagcatc ccgtcagacg
      181 aacgtttacg aactataaat caggtattac tggccctcga aatctggaga tactgacgta
      241 tcaacacggt tcagtaacac ccgatacctc ttccagactg tcaacaacga tgtctcttca
      301 tccgttgcga tc
//
LOCUS       DAZFXT010000500          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.503, whole genome shotgun sequence.
ACCESSION   DAZFXT010000500 DAZFXT010000000
VERSION     DAZFXT010000500.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.503"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>298)
                     /locus_tag="ACHKW0_005457"
     CDS             complement(<1..>298)
                     /locus_tag="ACHKW0_005457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major tail subunit"
                     /protein_id="HFO7478058.1"
                     /translation="DCTIKEVQFTAGQKQDIDVTTLCSTEQENINGLGASSEISMSGN
                     FYLNQAQNALRDAYDNDTVYAFKVQFPSGKGFKFLAEVRQHTWSSGTNGVVAATF"
BASE COUNT           70 a           85 c           76 g           67 t
ORIGIN      
        1 aaaacgttgc agccaccacg ccgttggtac cggatgacca ggtgtgctga cgcacttccg
       61 ccaggaactt aaagccctta ccggacggaa actgcacctt aaacgcatac accgtgtcat
      121 tgtcataggc atcacgcagg gcgttctggg cctgattcag ataaaaatta cccgacatgg
      181 aaatctcgga cgacgccccc agaccgttga tgttctcctg ctctgtggag cagagcgtgg
      241 tcacatcaat atcctgtttc tgaccggcgg tgaactggac ttccttgatg gtgcagtc
//
LOCUS       DAZFXT010000501          298 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.504, whole genome shotgun sequence.
ACCESSION   DAZFXT010000501 DAZFXT010000000
VERSION     DAZFXT010000501.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.504"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>298
                     /locus_tag="ACHKW0_005458"
     CDS             <1..>298
                     /locus_tag="ACHKW0_005458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HFO7478059.1"
                     /translation="MACEEANGREQLAATLAAMPEMTVEKARPILAASPQADAGPSLR
                     DQIMALDEAKGAEAQAEQLAACPGMTVESARAVLAAGSGKAEPVSASTTALFER"
BASE COUNT           63 a           82 c          104 g           49 t
ORIGIN      
        1 ttatggcgtg tgaagaagcg aacggtcgtg aacaactggc ggcaacgctg gcggcgatgc
       61 cggagatgac ggtggaaaaa gcccgcccga tcctggctgc ttcaccgcag gcggatgccg
      121 gaccctcact ccgtgatcag atcatggcac tggatgaggc aaaaggggct gaggcgcagg
      181 ctgaacagct ggctgcctgc ccgggaatga ctgtggagag cgcccgggct gtgctggctg
      241 cgggatcagg taaggcagaa ccggtctctg catccacaac cgccctgttt gaacgcat
//
LOCUS       DAZFXT010000502          293 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.505, whole genome shotgun sequence.
ACCESSION   DAZFXT010000502 DAZFXT010000000
VERSION     DAZFXT010000502.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..293
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.505"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>293
                     /locus_tag="ACHKW0_005459"
     CDS             <1..>293
                     /locus_tag="ACHKW0_005459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HFO7478060.1"
                     /translation="SGANEKISQQRRDLWKAENQYAVLKEAATKRQLSEQEKSLLTHE
                     KETLEYKRQLAELGDKVEHQKRLNELAQQAARFEQQQGAKQAAISAQARGLTDR"
BASE COUNT           86 a           72 c           97 g           38 t
ORIGIN      
        1 agcggtgcga atgagaaaat cagccagcag cgtcgcgatt tatggaaagc ggaaaatcag
       61 tatgcggtcc tgaaagaggc agccacgaaa cggcagttat ctgagcagga aaaatccctg
      121 ctgacccatg agaaagagac gctggagtac aaacgccagc tggctgagct gggagacaaa
      181 gttgaacacc agaaacggct gaatgagctg gcacagcagg ctgcgcggtt tgagcagcag
      241 cagggcgcga agcaggcggc aatcagtgcc caggcgcggg gcctcaccga ccg
//
LOCUS       DAZFXT010000503          289 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.506, whole genome shotgun sequence.
ACCESSION   DAZFXT010000503 DAZFXT010000000
VERSION     DAZFXT010000503.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..289
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.506"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..144)
                     /locus_tag="ACHKW0_005460"
     CDS             complement(<1..144)
                     /locus_tag="ACHKW0_005460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HFO7478061.1"
                     /translation="MGRRRSHERRDLPPNLYIRNNGYYCYRDPRTGKEFGLGRDRRIA
                     ITEA"
     gene            complement(122..>289)
                     /locus_tag="ACHKW0_005461"
     CDS             complement(122..>289)
                     /locus_tag="ACHKW0_005461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HFO7478062.1"
                     /translation="LETVRRWVRECRIFPPPVKDGREYLFHESAVKVDLNRPVTGSLL
                     KRIRNGKKAKS"
BASE COUNT           63 a           81 c           55 g           90 t
ORIGIN      
        1 tgcttcagtg attgctatcc tcctgtctcg gcctaaacca aactctttac ccgtccttgg
       61 gtccctgtag cagtaatatc cattgtttct tatataaagg ttagggggta aatcccggcg
      121 ctcatgactt cgccttcttc ccatttctga tcctcttcaa aaggctacct gttactggtc
      181 gatttaagtc aacctttacc gctgattcgt ggaacagata ctctcttcca tccttaaccg
      241 gaggagggaa tatcctgcat tcgcgcaccc atcgacgaac tgtttcaag
//
LOCUS       DAZFXT010000504          288 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.507, whole genome shotgun sequence.
ACCESSION   DAZFXT010000504 DAZFXT010000000
VERSION     DAZFXT010000504.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.507"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           76 a           59 c           79 g           74 t
ORIGIN      
        1 tgcgtcagtc agtgccatag ttcagccttc tgaatgcgtt aaagagggta caaattctgt
       61 cgaactgggg tatgcccgca attataccca cacaatgagg ttgatgtggg gagaatttgg
      121 ttgagttcgg tagaattgat ttggaggaag aacacttaaa tcatggtgtt ctgaaacgaa
      181 aaacggacct ccgtggaggt ccgttgatat gaatttggtg cccggactcg gaatcgaacc
      241 aaggacacgg ggattttcaa tcccctgctc taccgactga gctatccg
//
LOCUS       DAZFXT010000505          282 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.508, whole genome shotgun sequence.
ACCESSION   DAZFXT010000505 DAZFXT010000000
VERSION     DAZFXT010000505.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..282
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.508"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>282)
                     /locus_tag="ACHKW0_005462"
     CDS             complement(<1..>282)
                     /locus_tag="ACHKW0_005462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7478063.1"
                     /translation="RYIAAGLGVSYEQLSRDYSKVSYSSARASANESWRYFMGRRKFI
                     AARLATQMFSCWLEEALLRGIIRPPRARFDFYQARSAWSRAEWIGAGRMA"
BASE COUNT           74 a           90 c           70 g           48 t
ORIGIN      
        1 ggccattctt ccggcaccaa tccactctgc ccgtgaccag gctgatcgcg cctgataaaa
       61 atcaaaacgc gcccgtggcg gacggataat cccccgaaga agtgcctctt ccagccagca
      121 ggaaaacatc tgcgtggcca gccgggccgc aataaatttt cgccgcccca taaaatagcg
      181 ccacgactca ttggcagagg ccctggcact tgaataactg accttcgagt aatcacggga
      241 caactgttcg taggaaacgc caagaccggc ggcgatatac cg
//
LOCUS       DAZFXT010000506          281 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.509, whole genome shotgun sequence.
ACCESSION   DAZFXT010000506 DAZFXT010000000
VERSION     DAZFXT010000506.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.509"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..80
                     /locus_tag="ACHKW0_005463"
     CDS             <1..80
                     /locus_tag="ACHKW0_005463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478064.1"
                     /translation="QKIFVDLEKKYQVLLQVKFFTHLLI"
BASE COUNT           96 a           36 c           52 g           97 t
ORIGIN      
        1 tgcaaaagat ttttgtagac ctggagaaga agtatcaagt tctattgcaa gtaaagtttt
       61 tcacgcattt attgatttag ttaacaaaat caggggtaag aaagacttta tgtatatttg
      121 cacgctttgc tgttttgccg aggaggttaa aggtgattat tctcattaca ggaccttttt
      181 atttgatatt ggtaatcaat ataaggttaa acttacacaa agcggaaaaa aagagctctc
      241 tttaacttta gaatttaacg atactataat tgaatctcag a
//
LOCUS       DAZFXT010000507          274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.510, whole genome shotgun sequence.
ACCESSION   DAZFXT010000507 DAZFXT010000000
VERSION     DAZFXT010000507.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.510"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>274
                     /locus_tag="ACHKW0_005464"
     CDS             <1..>274
                     /locus_tag="ACHKW0_005464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7478065.1"
                     /translation="RHIKEVFILIPKKNSKSTLAAGIMMTALLLNWRQAAGYTILAPT
                     VEVAANAFNPARDMVRRDDDLDDLCQVQTHIRTITHRVTDTTLKVVA"
BASE COUNT           63 a           77 c           83 g           51 t
ORIGIN      
        1 cgccatatca aggaagtgtt tatccttatc cccaagaaaa acagtaagtc cacgctggct
       61 gcggggatca tgatgacggc gctgttactg aactggcggc aggcggcggg ctacaccatt
      121 ctggccccga ccgtggaggt ggcggctaac gccttcaacc ctgccaggga tatggtacga
      181 cgggacgatg atctggatga cctctgtcag gtgcagacac atatccggac catcacccat
      241 cgggtgacgg acaccaccct gaaggtggtg gcag
//
LOCUS       DAZFXT010000508          274 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.511, whole genome shotgun sequence.
ACCESSION   DAZFXT010000508 DAZFXT010000000
VERSION     DAZFXT010000508.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..274
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.511"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..137)
                     /locus_tag="ACHKW0_005465"
     CDS             complement(<1..137)
                     /locus_tag="ACHKW0_005465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1482 family protein"
                     /protein_id="HFO7478066.1"
                     /translation="MNTAFALVLTVFLVSGEPVDIAVSVHRTMQECVTAATEQKIPGN
                     C"
     gene            complement(134..>274)
                     /gene="dicB"
                     /locus_tag="ACHKW0_005466"
     CDS             complement(134..>274)
                     /gene="dicB"
                     /locus_tag="ACHKW0_005466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HFO7478067.1"
                     /translation="IGVTISNPVFTEDAINKRKHERELLNKICILSMLARLRPIQKGC
                     WQ"
BASE COUNT           76 a           59 c           54 g           85 t
ORIGIN      
        1 taacagttac cgggaatttt ctgttcggtt gctgcagtta cacactcctg cattgtcctg
       61 tgaacactga ctgcaatatc aactggctct ccggaaacaa gaaaaactgt cagaacaagt
      121 gcaaatgctg tattcattgc cagcatcctt tttgtatcgg acgtaaacgg gccagcattg
      181 aaagaatgca tattttattt aatagctccc gttcgtgttt tctcttgtta atggcatctt
      241 cagtaaatac agggttactg atagtgacac caat
//
LOCUS       DAZFXT010000509          273 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.512, whole genome shotgun sequence.
ACCESSION   DAZFXT010000509 DAZFXT010000000
VERSION     DAZFXT010000509.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..273
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.512"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(37..>273)
                     /gene="dinI"
                     /locus_tag="ACHKW0_005467"
     CDS             complement(37..>273)
                     /gene="dinI"
                     /locus_tag="ACHKW0_005467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HFO7478068.1"
                     /translation="EVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRYAAA
                     NNLSVIGATKEDKQRISEILQETWESADDWFVSE"
BASE COUNT           70 a           71 c           63 g           69 t
ORIGIN      
        1 ccggggcgac ccggcaaaaa caaaaatcac tgcatattat tcgctgacaa accagtcatc
       61 ggcgctttcc cacgtttcct ggagaatttc gctaatgcgc tgtttatctt cttttgttgc
      121 gccaataacc gataaattat tcgctgcggc ataacgtacc gatacgtggc cttcattatc
      181 aggaaacgca tactgaatac ggcgggaaag ttcgccagcc agggcgtcaa tagccccagc
      241 tggcaatgga gaagttttcg ctatggtgac ttc
//
LOCUS       DAZFXT010000510          272 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.513, whole genome shotgun sequence.
ACCESSION   DAZFXT010000510 DAZFXT010000000
VERSION     DAZFXT010000510.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..272
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.513"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT          120 a           55 c           27 g           70 t
ORIGIN      
        1 tccttaataa gacaacaata aaaaactaat tatataaaaa caataaaaat aatatacaaa
       61 aaaactaaac aacaaagaaa agcaaatcat atctggcatt ttaattgaaa gagcaattac
      121 cgaacaaaag acgctgacta tatactcaaa accaaacaac aattctgcca atcaggtatc
      181 atggcaacac acggaattac catgtttttg ccttctctgc ccatacaata cgggcatata
      241 cttcatactc tattgtaata tttctatcca tg
//
LOCUS       DAZFXT010000511          268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.514, whole genome shotgun sequence.
ACCESSION   DAZFXT010000511 DAZFXT010000000
VERSION     DAZFXT010000511.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.514"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>268
                     /locus_tag="ACHKW0_005468"
     CDS             <1..>268
                     /locus_tag="ACHKW0_005468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HFO7478069.1"
                     /translation="MPGRRPDSILKAGQHRYQRAFIQRLKNGRWHVMQRVAGKNRYPI
                     DVVKIPMAAPLKQAFDENVDRIRRERLPGELAYALKQQLRIAIKR"
BASE COUNT           72 a           66 c           76 g           54 t
ORIGIN      
        1 atgccggggc gtcgtccgga cagcatactc aaagccggtc agcatcgtta tcagcgggca
       61 tttattcagc gattaaaaaa tggtcgctgg catgtcatgc agcgtgtggc cgggaaaaac
      121 cgttacccca ttgatgtggt gaaaatcccg atggcggccc cactgaaaca ggcgtttgat
      181 gaaaacgttg accgtatccg gcgtgaacgc ctgcccggag aactggcata tgcgctgaaa
      241 caacaactga ggattgcgat aaaacgat
//
LOCUS       DAZFXT010000512          268 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.515, whole genome shotgun sequence.
ACCESSION   DAZFXT010000512 DAZFXT010000000
VERSION     DAZFXT010000512.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..268
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.515"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>268)
                     /locus_tag="ACHKW0_005469"
     CDS             complement(<1..>268)
                     /locus_tag="ACHKW0_005469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HFO7478070.1"
                     /translation="LSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKHCGVIN
                     PQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKH"
BASE COUNT           86 a           64 c           39 g           79 t
ORIGIN      
        1 cgtgttttaa taattcacat aataaactcc aacccttttc cctgatgcct atatgccctg
       61 aaacgagcaa tgcataaagt acagcgccta ctcttccatc tgtatcgcca aaataagccg
      121 tgttggcatt ttgtgggtta atcacaccac agtgtttata aaagtctatt ttagaaaaca
      181 cagatttaac atagctatca aacttatcct taggaaacgt ctctttagag acatcccaac
      241 gccagtcagc tttaaaatta ccgctaag
//
LOCUS       DAZFXT010000513          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.516, whole genome shotgun sequence.
ACCESSION   DAZFXT010000513 DAZFXT010000000
VERSION     DAZFXT010000513.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.516"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
BASE COUNT           89 a           56 c           33 g           89 t
ORIGIN      
        1 ggaccgccat actgagtcat atctccctga cgcgccgctg gttttccgct catataaagt
       61 gcccttttag ttattgttca tatccctgtg ttagcaacaa aaaaaataaa atgacacaca
      121 agaccagcta atgtatttat atatttttca cataaactta tttgttctca atcaacaaca
      181 actgttatta caggtcactt tcaatatttt attaaactaa agtttttagc acagatgaat
      241 tcatcatcaa caaattcact atatgga
//
LOCUS       DAZFXT010000514          267 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.517, whole genome shotgun sequence.
ACCESSION   DAZFXT010000514 DAZFXT010000000
VERSION     DAZFXT010000514.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..267
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.517"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>267
                     /locus_tag="ACHKW0_005470"
     CDS             <1..>267
                     /locus_tag="ACHKW0_005470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478071.1"
                     /translation="VFDSYSNEYANSGVAALLQYCSSKMDLPSNNDTYHEMYHALNMN
                     LSSKNFEDGHISTGTIFFDTESNKWYLCVSAACDLVPTQGNDPHH"
BASE COUNT           78 a           48 c           48 g           93 t
ORIGIN      
        1 gtttttgata gctattcaaa tgaatacgct aacagcggag ttgctgcatt gctccaatat
       61 tgctcttcaa aaatggatct gccatcaaat aatgatactt atcacgaaat gtatcatgct
      121 ttaaatatga atttgtcttc aaagaatttt gaagatggtc atatttctac tggcactatt
      181 ttctttgata cagagtcgaa caaatggtat ttatgtgtat ctgcggcatg tgatttggtt
      241 cctactcagg gtaacgaccc tcaccat
//
LOCUS       DAZFXT010000515          266 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.518, whole genome shotgun sequence.
ACCESSION   DAZFXT010000515 DAZFXT010000000
VERSION     DAZFXT010000515.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..266
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.518"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>266
                     /gene="bet"
                     /locus_tag="ACHKW0_005471"
     CDS             <1..>266
                     /gene="bet"
                     /locus_tag="ACHKW0_005471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage recombination protein Bet"
                     /protein_id="HFO7478072.1"
                     /translation="IALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINE
                     NQQFDGMDFEQDNESCTCRIYRKDRNHPICVTEWMDECRREPFKT"
BASE COUNT           70 a           67 c           68 g           61 t
ORIGIN      
        1 atcgcattgt tgatcgtcgc caaccagtac ggccttaatc cgtggacgaa agaaatttac
       61 gccttccctg ataagcagaa cggcatcgtt ccggtggtgg gcgttgatgg ctggtcccgc
      121 atcatcaatg aaaaccagca gtttgatggc atggactttg agcaggacaa tgaatcctgt
      181 acatgccgga tttaccgcaa ggaccgtaat catccgatct gcgttaccga atggatggat
      241 gaatgccgcc gcgaaccatt caaaac
//
LOCUS       DAZFXT010000516          263 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.519, whole genome shotgun sequence.
ACCESSION   DAZFXT010000516 DAZFXT010000000
VERSION     DAZFXT010000516.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.519"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>263)
                     /locus_tag="ACHKW0_005472"
     CDS             complement(<1..>263)
                     /locus_tag="ACHKW0_005472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030430 - host cell cytoplasm [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046718 - symbiont entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HFO7478073.1"
                     /translation="ATVVRRRVYARFLDAVNFVAGNPEADPEQELTDRWVVEQMSSLT
                     AMTASFVLATPTETDGALFPGRIMLANTCMWDYRSDECGYNGPA"
BASE COUNT           54 a           96 c           71 g           42 t
ORIGIN      
        1 gccggaccgt tatacccgca ttcatcagag cggtaatccc acatacaggt gttcgccagc
       61 atgatgcgac cgggaaacag cgccccgtcc gtctcggtcg gtgtggccag cacaaacgag
      121 gccgtcatgg ccgtcagcga tgacatctgc tccaccaccc accggtcagt cagctcctgc
      181 tccgggtcgg cctccggatt gcctgccaca aagttcaccg catccagaaa acgcgcatac
      241 acccggcggc ggaccaccgt ggc
//
LOCUS       DAZFXT010000517          259 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.520, whole genome shotgun sequence.
ACCESSION   DAZFXT010000517 DAZFXT010000000
VERSION     DAZFXT010000517.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..259
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.520"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(44..>259)
                     /locus_tag="ACHKW0_005473"
     CDS             complement(44..>259)
                     /locus_tag="ACHKW0_005473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF826 domain-containing protein"
                     /protein_id="HFO7478074.1"
                     /translation="EAVKEVLRSEEVRSALKQKLRHNLEARLDAEVDAILDELLGAPA
                     APEPEGIAGEGSASDSGDPTPDSDMMM"
BASE COUNT           49 a           87 c           62 g           61 t
ORIGIN      
        1 taaaaaagac caccggcaca caccgatggt ccctgacgca tgcttacatc atcatgtcgc
       61 tgtccggtgt ggggtcaccg ctatctgaag cactcccctc acccgcgatg ccttccggct
      121 ccggagctgc cggtgcgccc agcagttcat ccagaatggc atccacttct gcatcaagac
      181 gcgcttccag gttatggcga agtttctgtt tcagtgcgct ccggacttct tcagagcgca
      241 ggacttcctt cactgcttc
//
LOCUS       DAZFXT010000518          258 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.521, whole genome shotgun sequence.
ACCESSION   DAZFXT010000518 DAZFXT010000000
VERSION     DAZFXT010000518.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..258
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.521"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>258
                     /locus_tag="ACHKW0_005474"
     CDS             <1..>258
                     /locus_tag="ACHKW0_005474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001383906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HFO7478075.1"
                     /translation="INGWQKYVRFLRAFVDAYKNSDMIYTYLPGGDNCTLSADNRIFI
                     PVLGLGLINAVNEKDVIIVKQWRKYEGYRYLPCFDILKIQN"
BASE COUNT           88 a           34 c           53 g           83 t
ORIGIN      
        1 tataaacgga tggcaaaaat atgtacgctt cttacgtgca ttcgttgatg cgtataaaaa
       61 ttccgacatg atttatacat atttgcctgg tggtgataat tgtacgcttt cggcagacaa
      121 tagaatattc atacctgtac ttggtcttgg gctgattaat gcagtaaatg aaaaagatgt
      181 tataattgtt aagcagtggc gaaaatatga gggatataga tatctgccat gctttgatat
      241 attaaaaata cagaataa
//
LOCUS       DAZFXT010000519          255 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.522, whole genome shotgun sequence.
ACCESSION   DAZFXT010000519 DAZFXT010000000
VERSION     DAZFXT010000519.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.522"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(30..255)
                     /locus_tag="ACHKW0_005475"
                     /pseudo
     CDS             complement(30..255)
                     /locus_tag="ACHKW0_005475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308874.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M85 family metallopeptidase"
BASE COUNT           72 a           57 c           60 g           66 t
ORIGIN      
        1 ccatagctta cggcatcacg aaaagtttat gagcggctca aagcatcaag tatagtatta
       61 ctaatcatat cgaccgtatg ctgatcaatg agtctgccac gactgtcatg gacggcgcgt
      121 attacacttt cgtatacgtt gcttaaatct gctgcggaaa gtggtattcg cttacctata
      181 tccaaaacgt aatccgcata ggcattttca gcacgattag gagcaatggg agcagagtat
      241 cctgccgggg cggaa
//
LOCUS       DAZFXT010000520          251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.523, whole genome shotgun sequence.
ACCESSION   DAZFXT010000520 DAZFXT010000000
VERSION     DAZFXT010000520.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.523"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>251
                     /locus_tag="ACHKW0_005476"
     CDS             <1..>251
                     /locus_tag="ACHKW0_005476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HFO7478076.1"
                     /translation="QETFDEEYQVEVQEDDPEKMEGAEHPHKENTGGNQHHDSDNETG
                     ETADHSIKVNGHQEITSTSRTCDHLMIDLETMGKNPDAP"
BASE COUNT           90 a           55 c           63 g           43 t
ORIGIN      
        1 caggaaacat tcgatgaaga gtatcaggtt gaagttcagg aagatgatcc ggagaaaatg
       61 gaaggcgctg aacatccaca caaggagaac actggcggca atcagcatca cgatagcgat
      121 aatgaaactg gcgagacggc agatcactca attaaggtga acggtcatca agaaatcaca
      181 tccaccagca ggacgtgtga ccatctaatg atcgaccttg aaaccatggg aaaaaatcct
      241 gatgccccga t
//
LOCUS       DAZFXT010000521          251 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.524, whole genome shotgun sequence.
ACCESSION   DAZFXT010000521 DAZFXT010000000
VERSION     DAZFXT010000521.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..251
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.524"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>251
                     /locus_tag="ACHKW0_005477"
     CDS             <1..>251
                     /locus_tag="ACHKW0_005477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044384 - host outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HFO7478077.1"
                     /translation="RNRWFSVMAGPSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTD
                     NKGKTHDVLTGSDDGRHSNTSLAWGAGVQFNPTESVAIDV"
BASE COUNT           49 a           62 c           86 g           54 t
ORIGIN      
        1 cgtaaccgct ggttcagcgt aatggcgggg ccgtctgtgc gcgtgaatga atggttcagc
       61 gcgtatgcga tggcgggagt ggcttacagc cgtgtgtcga ctttctccgg ggattatctc
      121 cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga ccggaagtga tgacggtcgc
      181 cacagcaaca cgtctctggc gtggggggct ggcgtgcagt ttaacccgac cgaatccgtg
      241 gccattgatg t
//
LOCUS       DAZFXT010000522          246 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.525, whole genome shotgun sequence.
ACCESSION   DAZFXT010000522 DAZFXT010000000
VERSION     DAZFXT010000522.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..246
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.525"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..161
                     /locus_tag="ACHKW0_005478"
     CDS             <1..161
                     /locus_tag="ACHKW0_005478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HFO7478078.1"
                     /translation="FRAGETYHPGDTVTWGGSLWHCNSMTGDKPGEAHSSGWTLAAKR
                     GRDAGGGK"
     gene            158..>246
                     /locus_tag="ACHKW0_005479"
     CDS             158..>246
                     /locus_tag="ACHKW0_005479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail connector protein"
                     /protein_id="HFO7478079.1"
                     /translation="MTALLTLEEIKAHLRVDHDADDDMLMDKVR"
BASE COUNT           63 a           58 c           81 g           44 t
ORIGIN      
        1 tgttcagagc cggtgaaacc taccaccccg gcgatacggt gacgtggggg ggctcgctgt
       61 ggcactgcaa cagtatgacc ggtgataaac ccggagaagc tcattcatca ggctggaccc
      121 tggctgcaaa acgtggacgg gatgcaggag gcggaaaatg acggcattac tgacactgga
      181 agagatcaag gcacatctgc gtgttgacca tgacgcggat gatgacatgc tgatggacaa
      241 ggttcg
//
LOCUS       DAZFXT010000523          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.526, whole genome shotgun sequence.
ACCESSION   DAZFXT010000523 DAZFXT010000000
VERSION     DAZFXT010000523.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.526"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>245
                     /locus_tag="ACHKW0_005480"
     CDS             <1..>245
                     /locus_tag="ACHKW0_005480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7478080.1"
                     /translation="AEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARD
                     VRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMV"
BASE COUNT           58 a           68 c           70 g           49 t
ORIGIN      
        1 gcggaagaca tgctacgtga agccatcggc gggcttgcct cccgtccgga aggatttgtg
       61 gtgtacacaa ccacccagtc gaatgaaccg cctgccgggg tgttcagaca gaaactgcag
      121 tacgcccggg atgtccgtga cggcaaaatt catgatccgc actttctgcc ggtgatattt
      181 gaacaccctc ctgaaatggt ggaaagcggg gctcacctgc tgatggaaaa cctcgccatg
      241 gtcaa
//
LOCUS       DAZFXT010000524          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.527, whole genome shotgun sequence.
ACCESSION   DAZFXT010000524 DAZFXT010000000
VERSION     DAZFXT010000524.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.527"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..>245
                     /locus_tag="ACHKW0_005481"
     CDS             <1..>245
                     /locus_tag="ACHKW0_005481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="diguanylate cyclase regulator RdcB family
                     protein"
                     /protein_id="HFO7478081.1"
                     /translation="ADNRVCQVLTCLPEKFVVDFANGIDVAQEHIRTAGERTFFRRLK
                     EGLTGEGAARQNAINASLAQGVEASLRWLTEMTTSLA"
BASE COUNT           54 a           63 c           75 g           53 t
ORIGIN      
        1 tgctgataac cgggtatgtc aggtactcac ctgtcttcct gaaaaatttg tggttgattt
       61 cgcaaacgga atcgatgttg cacaggagca tatccgcacg gccggagagc gcacgttctt
      121 ccggcgttta aaagaggggc tgaccggcga aggcgctgca cggcagaatg ccatcaatgc
      181 gtcgcttgca cagggcgttg aggcgtccct gcgctggctg acggagatga caacgtcact
      241 tgcca
//
LOCUS       DAZFXT010000525          245 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.528, whole genome shotgun sequence.
ACCESSION   DAZFXT010000525 DAZFXT010000000
VERSION     DAZFXT010000525.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.528"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(<1..>245)
                     /locus_tag="ACHKW0_005482"
     CDS             complement(<1..>245)
                     /locus_tag="ACHKW0_005482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HFO7478082.1"
                     /translation="AEDMLREAIGGLASRPEGFVVYTTTQSNEPPAGVFRQKLQYARD
                     VRDGKIHDPHFLPVIFEHPPEMVESGAHLLMENLAMV"
BASE COUNT           53 a           73 c           65 g           54 t
ORIGIN      
        1 ttaaccatgg cgaggttttc catcagcagg tgagccccgc tttccaccat ttcaggagga
       61 tgctcaaaaa tcaccggcag aaagtgcgga tcatgaattt tgccgtcacg gacatcccgg
      121 gcatactgca gtttctgtct gaacaccccg gcgggcggtt cattcgactg ggtggtcgta
      181 tacaccacaa acccttccgg acgggaggca agcccgccga tggcttcacg tagcatgtct
      241 tccgc
//
LOCUS       DAZFXT010000526          236 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.denovo.529, whole genome shotgun sequence.
ACCESSION   DAZFXT010000526 DAZFXT010000000
VERSION     DAZFXT010000526.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.denovo.529"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            complement(11..236)
                     /locus_tag="ACHKW0_005483"
                     /pseudo
     CDS             complement(11..236)
                     /locus_tag="ACHKW0_005483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT           75 a           55 c           35 g           71 t
ORIGIN      
        1 tctttcccta ggatacaact ctggcaatgg tatcgtgaac aggaatacca ttttcaaaat
       61 caccatattg cttcaaaaaa tcgagatgtg tttccccgaa atcctctata tcttcccaac
      121 cttctgcacc agaaataacg gcacaaatag tcaacagtag aatatctgac aatttatgca
      181 ccactttcca ggcttgtctg taatcgggtg taatagaaat atgttccatc aatttt
//
LOCUS       DAZFXT010000527         3780 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.guided.237, whole genome shotgun sequence.
ACCESSION   DAZFXT010000527 DAZFXT010000000
VERSION     DAZFXT010000527.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.guided.237"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..485
                     /gene="prfH"
                     /locus_tag="ACHKW0_005484"
     CDS             <1..485
                     /gene="prfH"
                     /locus_tag="ACHKW0_005484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HFO7478083.1"
                     /translation="GRYSDTLRSALISLDGDNAWALSESWCGAIQWICPSPYRPHHGR
                     KNWFLGIGRFTADEQEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQS
                     ERSQHANKRLARLLIAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG
                     "
     gene            complement(542..1999)
                     /gene="pepD"
                     /locus_tag="ACHKW0_005485"
     CDS             complement(542..1999)
                     /gene="pepD"
                     /locus_tag="ACHKW0_005485"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HFO7478084.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLMNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2260..2718
                     /gene="gpt"
                     /locus_tag="ACHKW0_005486"
     CDS             2260..2718
                     /gene="gpt"
                     /locus_tag="ACHKW0_005486"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HFO7478085.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            2810..>3780
                     /gene="frsA"
                     /locus_tag="ACHKW0_005487"
     CDS             2810..>3780
                     /gene="frsA"
                     /locus_tag="ACHKW0_005487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HFO7478086.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASR"
BASE COUNT          876 a          966 c         1002 g          936 t
ORIGIN      
        1 cgggccgcta ctctgacacg ctgcgttcgg cgctgatttc tctggatggc gataacgcat
       61 gggcattaag cgaaagctgg tgtggcgcta ttcagtggat ttgtccgagt ccgtatcggc
      121 ctcatcatgg gcgcaaaaac tggtttctgg gcattgggcg ttttaccgct gatgagcagg
      181 aacaatcgga tgcaatccgt tatgagacgt tgcgttcgtc ggggccgggc ggtcaacatg
      241 tcaataaaac cgactcggcg gtacgcgcca cgcatttggc atccggtatt agcgtgaagg
      301 ttcagtcaga gcgtagtcag catgctaaca agcggctggc acgattgctg attgcctgga
      361 agctggagca gcagcaacag gaaaatagcg cggtgctgaa atcgcagcgg cgaatgttcc
      421 atcaccagat tgaacgtggc aacccgagac ggacatttac agggatggct tttatcgaag
      481 gataatgaag gatgaaactg ccggaaggcg attaaacgcc atccggcagc aaatcaaata
      541 attacttcgc cggaatttct ttcagcagtt cagtcagcag cgtccagtaa tgacctacgc
      601 tttcgatgtg aacttgctca tccggagagt gtggaccggt gatagttggc ccgatagaaa
      661 ccatgtccat ttccggatac ggttttttga acagaccaca ttccaggccc gcgtggataa
      721 tctggatgtt cggtgttttg ttgaacagac gctgataggt ttcacgtacc agatgcatca
      781 ccggagaatt agcgtccggc tgccagccag gatatgcgcc tttcgcttcg gttttcgcgc
      841 cagccagttt acccagcgaa tccagcatgc tcacgacgta gtctttaccg ctgtcgatca
      901 gtgaacggat cagacagtga atttctacgt tattatcagt catggtcacc acaccgacgt
      961 tcagggaggt ttcgaccaca ccttttgcca cgtctgagtt gcggatcaca ccgttcgggg
     1021 tggcgttcag cagacgaata aaggtatcgc gagatttcgc aatcagggca gctttatcgt
     1081 tcgctacaga gtccagcaac aaggccagat ttttctcttt ctctgccagc tcgtttttca
     1141 ggatctcctg ataggtattc atcagagatt tcaggacgtc gactttatca gctgcgacag
     1201 caatggtcgc aaaggcttca cgcgggatgg cgttacgcag tgtgccgccg ttgaaatcga
     1261 taaggcgcag atccagttct tctgcatgac ccgccaggaa gcgcaccagc agtttgttgg
     1321 cattacccag gccaacgtgg atttccccgc cggagtgacc ccctttcaga ccttttaagg
     1381 ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg atctaaatgc aggttggagg
     1441 tgaagtcgat accccccgca caacccatgt agatttcacc ttcttcttcg gagtcggtgt
     1501 taatcagaat atcagcctgc aaccagttgc tctgtaagcc gaacgcaccg tccataccgg
     1561 cttcttcggt catggtcagc agcacttcca gcgggccgtg aaccacgttt tcgtcagcca
     1621 gaactgccag tgcagaggcc ataccaatgc cgttatccgc acccagcgtg gtgccgcgcg
     1681 ctttaaccca ttcgccatca atataaggct ggataggatc tttcgtgaag tcatgcacgg
     1741 tgtcgttatt tttctgcggc accatatcga ggtgggcctg taagacgacc ggtttacgat
     1801 tttccatacc tgcggtagca ggtttacgaa tcaggatatt acctacctga tcgcgttcga
     1861 catggaaacc tttctctttt gcccaaccaa caatgtattc agcgagttgc tcttcatgat
     1921 aggacgggtg aggaatagaa cagattttgg caaaaatatc ccacagcggc tgtggagata
     1981 attgagacag ttcagacacg ttaagtctcc ttgtcgatca cccgcaaaac agtattgcag
     2041 gtcacagggt tagcagaaaa tgttgtcaac acaagacagg cttgcgagat atgtttgaga
     2101 ataccacttt atcccgcgtc agggagaggc agtgcgtaaa aagacgcgga ctcatgtgaa
     2161 atactggttt ttagtgcgcc agatctctat aatctcgcgc aacctatttt cccctcgaac
     2221 actttttaag ccgtagataa acaggctggg acacttcaca tgagcgaaaa atacatcgtc
     2281 acctgggaca tgttgcagat ccatgcacgt aaactcgcaa gccgactgat gccttctgaa
     2341 caatggaaag gcattattgc cgtaagccgt ggcggtctgg taccgggtgc gttactggcg
     2401 cgtgaactgg gtattcgtca tgtcgatacc gtttgtattt ccagctacga tcacgacaac
     2461 cagcgcgagc ttaaagtgct gaaacgtgca gaaggcgatg gcgaaggctt catcgttatt
     2521 gatgacctgg tggataccgg tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg
     2581 cactttgtca ccatcttcgc aaaaccggcc ggtcgcccgc tggttgatga ttatgttgtt
     2641 gatatcccgc aagatacctg gatcgaacag ccgtgggata tgggcgtcgt attcgtcccg
     2701 ccaatctccg gtcgctaatc ttttcaacgc ctggcactgc cgggcgttgt tctttttaac
     2761 ttcaggcggg ttacaatagt ttccagtaag tattctggag gctgcatcca tgacacaggc
     2821 aaacctgagc gaaaccctgt tcaaaccccg ctttaaacat cctgaaacct cgacgctagt
     2881 ccgccgcttt aatcacggcg cacaaccgcc tgtgcagtcg gctcttgatg gtaaaaccat
     2941 ccctcactgg tatcgcatga ttaaccgtct gatgtggatc tggcgcggta ttgacccacg
     3001 tgaaatcctc gacgtccagg cacgtattgt gatgagcgat gctgaacgta ccgacgatga
     3061 tttatacgat actgtgattg gctaccgtgg tggcaactgg atttatgagt gggccaccca
     3121 ggcgatggtg tggcaacaaa aagcctgtgc ggaagaagat ccgcaactca gtggtcgaca
     3181 ctggctgcat gcggctacgt tgtacaacat tgccgcctat cctcatctga aaggagatga
     3241 cctggccgag caagcgcagg ctttatcaaa ccgcgcctat gaagaggccg cccagcgtct
     3301 gccgggcacg atgcggcaga tggagtttac cgtacccggc ggtgcgccca tcaccggctt
     3361 tttgcatatg ccgaaaggcg atggcccgtt cccgacagta ttaatgtgtg gtggtctgga
     3421 tgcgatgcag acggactatt acagcctgta tgaacgttat tttgcgccgc gcggcattgc
     3481 gatgctgact attgatatgc cgtcggtggg cttttcttca aaatggaagc tcacccagga
     3541 ctccagcctg ttgcatcagc acgtcttaaa ggcgctgcct aacgtaccgt gggtggatca
     3601 cactcgcgtc gcggcctttg gtttccgttt cggcgctaac gttgccgtgc gtctggcata
     3661 ccttgaatcg ccgcgtctga aagcggttgc ctgtcttggt ccggtagttc ataccctgtt
     3721 gagtgatttt aagtgccagc aacaggtgcc ggaaatgtat cttgacgttc tggcgagtcg
//
LOCUS       DAZFXT010000528         2985 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.guided.254, whole genome shotgun sequence.
ACCESSION   DAZFXT010000528 DAZFXT010000000
VERSION     DAZFXT010000528.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.guided.254"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            <1..183
                     /locus_tag="ACHKW0_005488"
     CDS             <1..183
                     /locus_tag="ACHKW0_005488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HFO7478087.1"
                     /translation="KSGNLVPYRVELINRIGQEAVDEIESNHNRHRWTVEECRAIKAK
                     YQQKLKDLRNSRSEAA"
     gene            180..374
                     /locus_tag="ACHKW0_005489"
     CDS             180..374
                     /locus_tag="ACHKW0_005489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HFO7478088.1"
                     /translation="MTFTVKTIPDMLLEAYGNQTEVARILNCNRATVRKYIGDKEGKK
                     HAVVNGVLMVHRGWGKDTDA"
     gene            367..801
                     /locus_tag="ACHKW0_005490"
     CDS             367..801
                     /locus_tag="ACHKW0_005490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0060567 - negative regulation of
                     termination of DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator Q family protein"
                     /protein_id="HFO7478089.1"
                     /translation="MRDIRQVLERWGAWAANNHEDVTWSPIAAGFKGLIPEKVKSRPQ
                     CCDDDAMIICGCMARLNRNNSDLHDLLVDYYVLGETFMALARKHGCSDTCIGKRLHKA
                     EGIVEGMLMMLGVRLEMDRYVERELPGGRTSVFYQRKNSLRS"
     gene            1308..2255
                     /gene="stxA1a"
                     /locus_tag="ACHKW0_005491"
     CDS             1308..2255
                     /gene="stxA1a"
                     /locus_tag="ACHKW0_005491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit A"
                     /protein_id="HFO7478090.1"
                     /translation="MKIIIFRVLTFFFVIFSVNVVAKEFTLDFSTAKTYVDSLNVIRS
                     AIGTPLQTISSGGTSLLMIDSGTGDNLFAVDVRGIDPEEGRFNNLRLIVERNNLYVTG
                     FVNRTNNVFYRFADFSHVTFPGTTAVTLSGDSSYTTLQRVAGISRTGMQINRHSLTTS
                     YLDLMSHSGTSLTQSVARAMLRFVTVTAEALRFRQIQRGFRTTLDDLSGRSYVMTAED
                     VDLTLNWGRLSSVLPDYHGQDSVRVGRISFGSINAILGSVALILNCHHHASRVARMAS
                     DEFPSMCPADGRVRGITHNKILWDSSTLGAILMRRTISS"
     gene            2265..2534
                     /gene="stxB1a"
                     /locus_tag="ACHKW0_005492"
     CDS             2265..2534
                     /gene="stxB1a"
                     /locus_tag="ACHKW0_005492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Shiga toxin Stx1a subunit B"
                     /protein_id="HFO7478091.1"
                     /translation="MKKTLLIAASLSFFSASALATPDCVTGKVEYTKYNDDDTFTVKV
                     GDKELFTNRWNLQSLLLSAQITGMTVTIKTNACHNGGGFSEVIFR"
BASE COUNT          811 a          565 c          761 g          848 t
ORIGIN      
        1 aaaagcggaa atctcgttcc gtatcgcgtc gaactgatta accgcatcgg gcaggaagca
       61 gtagacgaaa tcgaatcgaa ccataaccgc catcgctgga ctgtcgaaga atgcagggcg
      121 atcaaggcga agtatcagca gaaacttaaa gacctgcgaa acagcagaag tgaggccgca
      181 tgacgttcac agtaaaaacc attcctgaca tgctccttga ggcatatgga aatcagaccg
      241 aggtagcccg aatactgaac tgcaatcgtg ccacagtcag aaaatacatt ggcgataaag
      301 aagggaaaaa gcacgccgtc gtcaatggcg tccttatggt tcatcgcgga tggggtaaag
      361 atactgatgc gtgatatccg gcaggttctt gagcgctggg gggcatgggc ggcaaataac
      421 catgaggatg ttacatggtc gcccattgct gccggattta agggactgat ccccgaaaaa
      481 gtaaaatcac gtccacagtg ttgtgacgat gacgcgatga ttatatgcgg gtgtatggct
      541 cgccttaaca ggaacaacag cgatctgcat gacttgctgg ttgattatta cgtgttgggg
      601 gagacgttca tggcgctggc acggaaacat gggtgctctg acacctgtat aggtaaacgc
      661 cttcacaaag cggaggggat tgttgaaggc atgctgatga tgctgggagt gaggcttgag
      721 atggatcggt atgttgagcg tgaattgccg ggagggagaa cctctgtatt ttatcagcga
      781 aaaaatagtt tacgatcgta aaaatctgca tatcatgata agagtggtta cattgccacg
      841 ctgcttaacc cgccgatgcg cgggtttttt tgtacccaga atcctgtgag ctatacggaa
      901 agtacacaga aaggaaggtg cgaccacaat taataacaaa atcttaaaaa ttgcacatgg
      961 cactattagt tttctaaata ttgtgtattt tttgtattgc aggatgaccc tgtaacgaag
     1021 tttgcgtaac agcattttgc tctacgagtt tgccagcctc ccccagtggc tggctttttt
     1081 atgtccgtaa catcctgtgt atcaataaat gttgttatct acgtacgtca agtagtcgca
     1141 tgagatctga ccagatatgt taaggttgca gctctctttg aatatgatta tcattttcat
     1201 tacgttattg ttacgtttat ccggtgcgcc gtaaaacgcc gtccttcagg gcgtggagga
     1261 tgtcaagaat atagttatcg tatggtgctc aaggagtatt gtgtaatatg aaaataatta
     1321 tttttagagt gctaactttt ttctttgtta tcttttcagt taatgtggtg gcgaaggaat
     1381 ttaccttaga cttctcgact gcaaagacgt atgtagattc gctgaatgtc attcgctctg
     1441 caataggtac tccattacag actatttcat caggaggtac gtctttactg atgattgata
     1501 gtggcacagg ggataatttg tttgcagttg atgtcagagg gatagatcca gaggaagggc
     1561 ggtttaataa tctacggctt attgttgaac gaaataattt atatgtgaca ggatttgtta
     1621 acaggacaaa taatgttttt tatcgctttg ctgatttttc acatgttacc tttccaggta
     1681 caacagcggt tacattgtct ggtgacagta gctataccac gttacagcgt gttgcaggga
     1741 tcagtcgtac ggggatgcag ataaatcgcc attcgttgac tacttcttat ctggatttaa
     1801 tgtcgcatag tggaacctca ctgacgcagt ctgtggcaag agcgatgtta cggtttgtta
     1861 ctgtgacagc tgaagcttta cgttttcggc aaatacagag gggatttcgt acaacactgg
     1921 atgatctcag tgggcgttct tatgtaatga ctgctgaaga tgttgatctt acattgaact
     1981 ggggaaggtt gagtagcgtc ctgcctgact atcatggaca agactctgtt cgtgtaggaa
     2041 gaatttcttt tggaagcatt aatgcaattc tgggaagcgt ggcattaata ctgaattgtc
     2101 atcatcatgc atcgcgagtt gccagaatgg catctgatga gtttccttct atgtgtccgg
     2161 cagatggaag agtccgtggg attacgcaca ataaaatatt gtgggattca tccactctgg
     2221 gggcaattct gatgcgcaga actattagca gttgaggggg taaaatgaaa aaaacattat
     2281 taatagctgc atcgctttca tttttttcag caagtgcgct ggcgacgcct gattgtgtaa
     2341 ctggaaaggt ggagtataca aaatataatg atgacgatac ctttacagtt aaagtgggtg
     2401 ataaagaatt atttaccaac agatggaatc ttcagtctct tcttctcagt gcgcaaatta
     2461 cggggatgac tgtaaccatt aaaactaatg cctgtcataa tggaggggga ttcagcgaag
     2521 ttatttttcg ttgactcaga atagctcagt gaaaatagca ggcggagatt cataaatgtt
     2581 aaatacatct caattcagtc agttgttgcc ggtctgataa tagatgtgtt agaaaatttc
     2641 tgcatggtga atccccctgt gcggaggggc gactggtgaa cggtatgatc tctttgatga
     2701 tcgtaagcga gaatacgcgg gtttggtggc accaggccga actcaccggg aggcacccgg
     2761 catcatgctg tatacagaga ttaggcatat atccaggctc ctcatcgcag gagccttttt
     2821 acatgcaaaa aaaagcccga gtgggttcgg gcaacagcat gagatacttg cattgtcatt
     2881 tttatcgtgc ggattttaac caggattcat aaggctgcgc aactgcgcgg cctttttcgt
     2941 atttcgggct gtagtccccg tgtgtcattc aggcttccgg actac
//
LOCUS       DAZFXT010000529         2648 bp    DNA     linear   BCT 22-OCT-2024
DEFINITION  TPA_asm: Escherichia coli isolate 2303F4962
            SAMN43283660-rid23078723.guided.270, whole genome shotgun sequence.
ACCESSION   DAZFXT010000529 DAZFXT010000000
VERSION     DAZFXT010000529.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN43283660
            Sequence Read Archive: SRR30325250
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (02-SEP-2024) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 02-SEP-2024
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002351512.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002351512.1
            Genome Coverage       :: 89x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 10/22/2024 07:17:46
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Annotation Software revision      :: 2024-07-18.build7555
            Genes (total)                     :: 5,497
            CDSs (total)                      :: 5,418
            Genes (coding)                    :: 5,193
            CDSs (with protein)               :: 5,193
            Genes (RNA)                       :: 79
            rRNAs                             :: 2, 4 (16S, 23S)
            partial rRNAs                     :: 2, 4 (16S, 23S)
            tRNAs                             :: 66
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 225
            CDSs (without protein)            :: 225
            Pseudo Genes (ambiguous residues) :: 0 of 225
            Pseudo Genes (frameshifted)       :: 96 of 225
            Pseudo Genes (incomplete)         :: 124 of 225
            Pseudo Genes (internal stop)      :: 55 of 225
            Pseudo Genes (multiple problems)  :: 46 of 225
            CRISPR Arrays                     :: 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2648
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN43283660-rid23078723.guided.270"
                     /serotype="O103:H 2"
                     /isolate="2303F4962"
                     /host="Homo sapiens"
                     /db_xref="taxon:562"
                     /geo_loc_name="Denmark"
                     /lat_lon="55.66267 N 12.587343 E"
                     /collection_date="2023-03"
                     /collected_by="SSI"
                     /note="subtype: stx1a"
     gene            26..1159
                     /locus_tag="ACHKW0_005493"
     CDS             26..1159
                     /locus_tag="ACHKW0_005493"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HFO7478092.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            1248..1781
                     /gene="blc"
                     /locus_tag="ACHKW0_005494"
     CDS             1248..1781
                     /gene="blc"
                     /locus_tag="ACHKW0_005494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HFO7478093.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1778..2095)
                     /gene="sugE"
                     /locus_tag="ACHKW0_005495"
     CDS             complement(1778..2095)
                     /gene="sugE"
                     /locus_tag="ACHKW0_005495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HFO7478094.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAIKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2277..2423)
                     /gene="ecnB"
                     /locus_tag="ACHKW0_005496"
     CDS             complement(2277..2423)
                     /gene="ecnB"
                     /locus_tag="ACHKW0_005496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HFO7478095.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2534..>2648)
                     /gene="ecnA"
                     /locus_tag="ACHKW0_005497"
     CDS             complement(2534..>2648)
                     /gene="ecnA"
                     /locus_tag="ACHKW0_005497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HFO7478096.1"
                     /translation="LIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
BASE COUNT          704 a          672 c          657 g          615 t
ORIGIN      
        1 ccgttttgta tggaaaccag accttatgtt caaaacgacg ctctgcgcct tattaattac
       61 cgcctcttgc tccacatttg ctgcccctca acaaatcaac gatattgtgc atcgcacaat
      121 taccccgctt atagagcaac aaaagatccc cggtatggcg gtggcggtaa tttatcaggg
      181 taaaccttat tactttacct ggggctatgc ggacatcgcc aaaaagcagc ccgtcacaca
      241 gcaaacgttg tttgagttag gttcggtcag caaaacattt acgggcgtgc ttggtggcga
      301 cgctattgct cgaggggaaa tcaagttaag cgatcccaca acaaaatact ggcctgaact
      361 taccgctaaa cagtggaatg ggatcacact attacatctc gcaacctaca ctgctggcgg
      421 cctgccattg caggtgccgg atgaggtgaa atcctcaagc gacttgctgc gcttctatca
      481 aaactggcag cctgcatggg cgccaggaac acaacgtctg tatgccaact ccagtatcgg
      541 tttgttcggc gcactggctg tgaagccgtc tggtttgagt tttgagcagg cgatgcaaac
      601 acgtgtcttc cagccactca aactcaacca tacgtggatt aatgtaccgc ccgcagaaga
      661 aaagaattac gcctggggat atcgcgaagg caaggcagtt catgtttcgc caggggcgtt
      721 agatgctgaa acttatggtg tgaagtcgac cattgaagat atggcctgct gggtacgaag
      781 caatatgaat ccccgtgata tcaacgacaa aacacttcag caagggatac aactggcaca
      841 atctcgctac tggcaaaccg gcgatatgta tcagggcctg ggctgggaaa tgctggactg
      901 gccggtaaat cctgacagca tcattaacgg cagtggcaat aaaattgcac tggcagcaca
      961 ccccgtaaaa gcgattacgc ccccaactcc tgcagtacgc gcatcatggg tacataaaac
     1021 aggggcaacc ggcggatttg gtagctatgt cgcgtttatt ccagaaaaag agctgggtat
     1081 agtgatgctg gctaacaaaa actatcccaa tccagcgaga gtcgccgccg cctggcagat
     1141 tctcaacgct ctacagtaaa atttcatcgg gtccgaattt tcggaccttt tctgcgcttt
     1201 tccttgctgt catctacact tagaaaaaaa ccagtaagga aacatttatg cgcctgctcc
     1261 ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc ctgcagttct cctacgccgc
     1321 cgcgtggcgt aacagtagtg aataatttcg acgccaaacg ttatctcggt acctggtatg
     1381 agattgcccg ttttgatcac cgttttgaac gtggactgga aaaagtcacc gcaacataca
     1441 gcctgcgtga tgacggcggc ctgaatgtca ttaataaagg ctataaccct gacagaggaa
     1501 tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc accaactcgc gctgcgctga
     1561 aagtgtcatt ctttggtcct ttctatggcg gttataacgt tattgcactc gatcgggaat
     1621 accgccatgc gctggtttgc ggcccggacc gcgactacct gtggatactc tcccgcacgc
     1681 caaccatttc tgacgaagtg aaacaggaga tgctggcagt cgcgacccgg gaagggtttg
     1741 atgtcagtaa gtttatttgg gtacagcagc ccggtagtta gtgagtgctg agtttcagac
     1801 caataatccc caatacgatt aacgccagac tcgccaggcg catcgggtta gcggactcac
     1861 cgagcagcac aatgccggtg atagccgcgc cgactgcgcc aatacccgtc cacacggcat
     1921 aagccgtccc tactggtaac gatttaatcg cccaggcaag tagcgccata ctgacaatca
     1981 tcgccgtcac ggtaataaca ctcggcgtca aacgactaaa gccgtgggta tatttcaggc
     2041 caacggccca taccacttcc agcagaccag caataactaa gataatccag gacatatcag
     2101 gctccggaga attggggccg tccccggtga aaagaggcgt ttgcaggtcg tcctgcaaag
     2161 ctgatggttg aacggatatt ttgccaggtg gcaggggaat ttcaactatt accttagtga
     2221 ctatcgcaga aaaaacaaac ggcacgacat acctgtcgta ccgttattgc ttattgctat
     2281 tgctgcgctt tcgttgctgc gccagaaatc gcgttaccgc catcagaaat gtcttcacca
     2341 acgccacgcg tggtgttgca ggcagttaat actgttgaaa gcaccagaac agaaaagatc
     2401 gctgcaattg tcttcttcac cataacgtct tccttttagg taatgttatt tatgtttgcc
     2461 tatagcaaat taataataga caacatcgcc aaaaatgacg gaaatctgat gatttttcgg
     2521 aagagaagaa aaattagctg gcagcgcgag agatggagtt gccgagatgt ttgatgtctt
     2581 cgccgaaacc gcgagcggtg ttacagcccg tgagcagcgt gctggcaagc aaaacaagaa
     2641 cgataagg
//
